LOCUS       DAORUQ010000001        69433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_177_length_69433_cov_6.347632, whole genome shotgun sequence.
ACCESSION   DAORUQ010000001 DAORUQ010000000
VERSION     DAORUQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 69433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69433
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_69433_cov_6.347632"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLW13_00005"
     CDS             complement(<1..130)
                     /locus_tag="PLW13_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HQY68776.1"
                     /translation="MSTARKETTGRTLLVALVLCIVCSVIVAGAAVSLKPVQTSNKQ"
     gene            complement(117..1376)
                     /locus_tag="PLW13_00010"
     CDS             complement(117..1376)
                     /locus_tag="PLW13_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HQY68777.1"
                     /translation="MKALRRMLDRVEPHFEKGGRFHSLYPLYEALDTFFYAPASVTRA
                     NAHVRDGIDLKRVMITVWLAALPAMFWGMYNLGFQANSAMQEGGVLVKTDWHGWLIGL
                     AAGYDPNSIWDCFWHGACYFLPIYIVTFAVGIAWEILFAAVRGHEVNEGFFVTSILFA
                     LTLPPDIPLWQVALGISFGVVVGKEVFGGTGKNFLNPALVGRAFLFFAYPAEISGDSV
                     WTAFDWAVVDGYSGASSMGVDGMSGATALSLGANGGLEAITAQFSWMQAFLGQIPGSI
                     GEVSKLAILLGAVLLLWTGIASWRIMLGVVIGTVAMGLLFNAVGSDTNPMFALPFYWH
                     FVIGGWAFGTVFMATDPVSAAMTDTGKLAFGILIGVMVMLIRVVNPAYPEGMMLAILF
                     ANIFAPLIDNFVMRANINRRLARHVNG"
     gene            complement(1378..2721)
                     /locus_tag="PLW13_00015"
     CDS             complement(1378..2721)
                     /locus_tag="PLW13_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HQY68778.1"
                     /translation="MITIRRGLDLPISGEPEQRVHEGPRVRTVAVLGPDYHGMKPSMT
                     VQPGDRVAKGQVLFTDRKVEGVQFTAPVAGTIAAINRGPKRVLLSVVIDVEGTEEREF
                     ERYEAAALASLSRERVCRNLCASGLWTALRTRPYSKVPAPASVPHAIFVTAIDTHPLA
                     ADPAVVLAPRAEEFRHGVQLLSALTEGKVYVVSGAGASVPRIEAPRVVHEEFGGPHPA
                     GLAGTHIHFLDPVHEHRAVWTVGYQDVVAIGHLFTTGRLLTERVVALAGPGIAKPRLL
                     RTVLGASTEELCAGELAPGEQRIISGSVLGGRRAHGALAFLGRYHTQVSVLPEGRERR
                     FMGWMSPGVDRHSVMGIYLSRFLGGRRFAMDTSTNGSPRAIVPIGAYEKVMPLDVLAT
                     PLLKSLVVGDVETAVKLGALELDEEDLALCSYVCPGKYEFGPILRDNLTRIESEA"
     gene            complement(2794..4290)
                     /locus_tag="PLW13_00020"
     CDS             complement(2794..4290)
                     /locus_tag="PLW13_00020"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQY68779.1"
                     /translation="MPDRKRPKPADYFSDWKQREALAEAMIPVVGKLYRERNVKVYVY
                     GRSLVSQSVLDIMKTHRFVRQVEHNELSEFETWPLVEALGRLELGTGHVDIGRLSVAY
                     AAGPRTEGVSAEAFLRKELQGLIGSTAKPVAAPRDVVLYGFGRIGRLMARLLIEKTGG
                     GDVLRLRAVVVRKSSDDDLVKRAALLRRDSIHGSFNGTIRVDEENGSFIANGNEVKLI
                     YADAPESIDYTAYGINDAIVIDNTGKWRDEAGLSRHLQARGVSKVILTAPGKGALKNI
                     VYGVNHADISADDRIITAASCTTNAIAPALKAVDEQFGIVSGHVETVHAYTNDQNLID
                     NFHKANRRGRSAPLNMVLTETGAASAVAKVLPSMAGKLTGNAIRVPVPDVSMAILHLT
                     LKRPTSVEEVNEFMRWASLHSPLSRQIDYTSSPEVVSSDFVGSRAACIHDAQATIVQG
                     NSAILYLWYDNEFGYACQVHRVLEHVAGVHYAVYPDATTVGFSWPKSR"
     gene            4438..7959
                     /gene="mfd"
                     /locus_tag="PLW13_00025"
     CDS             4438..7959
                     /gene="mfd"
                     /locus_tag="PLW13_00025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQY68780.1"
                     /translation="MNTTLLSPPRARPGDHLRWGGLIPGADALALASVAREHPGTMVV
                     LTAGSATAERIEYDLRFFLAGAGTAKPVLRLPDWETLPYDRFSPHQDLVSDRLLALSR
                     LAQGDPVVLIVPVATLMQRTAPVAYVSASSLALDPGQRLGVAQLRNTLARAGYRAVDN
                     VYEHGEFAVRGSIVDLFPMGHDTPVRIELFDEEIETLRSFDPETQRSLAPLERLRLLP
                     GREYPFDDAAIAAFRRRWHERFDNDHRACPDYQDVARGIAPGGIEYFLPLFFDQCATL
                     FDYLPEQSLLCIEDAARAGIDQVWEAVNGRYRSLGSDITRPLLAPAEVFLAPDELRAA
                     LKRHTRIEFTALEQPERAGAHNFGSGELALSREAGKRDDPVARIAAIAALPQARRKLF
                     CAETPGRREMLVELLARAGVKAEAVAGWEEFLRGEAPCAITIAPIAESFRLDEPSCLV
                     VAESALFGKRVMQRRRRSRAAESAETSIRNLAELDIGSPVVHLEHGVGRYLGLQTLEV
                     DGERAEFLCIEYAEAAKLFVPVTALDMVSRYTGIDTDAVTLNRLGSDQWARARRKAQE
                     KIRDVAAELLEIYARRESRPGHAFAHPGAAYESFAASFPFEETPDQLGAIEAVMNDMC
                     APRAMDRLVCGDVGFGKTEVAMRAAFIAVHSGKQVAVLVPTTLLAQQHFETFSDRFAD
                     LPVTIDSISRFRSASEQKQVLERVAAGRVDILIGTHGLLGTELRYRDLGLLVIDEEHR
                     FGVRQKDQLKALRATVDILTLTATPIPRTLNMAVAGLRDLSIISTPPARRLAVKTFVQ
                     RHDEGVVKDALDRELRRGGQVFFLHNDVKTIDRRAAEIERLIPEARVTVAHGQMRERE
                     LERVMSDFYHKRFNVLCCTTIIETGIDIPSANTIVIERADKFGLAQLHQLRGRVGRSH
                     HQAYAFLLTPDEKSMTPDAVKRLDAIAGTDSLGAGFLLATHDLEIRGAGELLGEEQSG
                     QIQAIGYSLYMDLLARAVEAMRSGRLVEPELALRPAIEINLRVPALIPDDYLPDVHNR
                     LIMYKRIASAASDEELHRLEVEMIDRFGLIPPPTRTLMRIARLRVAAQPLGIGKIEAS
                     ANGGVVEFASQTSVDPLTIVDLVQHDPRRYRLDGGTRLRFIEATEGVEARIACVEQLL
                     ARLLTPRAGATPPASQSRKAAARRAGR"
     gene            7956..>8853
                     /locus_tag="PLW13_00030"
     CDS             7956..>8853
                     /locus_tag="PLW13_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HQY68781.1"
                     /translation="MRAHRAFALLAALLPTPALLAQDIPAPAAAMAPWIQVELIVFRN
                     LDTTSAAGEQWPAQPALAYPATLRFLLDPGGMPAPDAPAPAATMATATPEPVTPTAPV
                     PFALLASPESLLGDAAARLAGSPGHRVLAHLSWRQPAPGHGAEHILVTGGRPWGEHRE
                     LEGYVSVSRANFVHVATHLWLNDFADAAAGQAPPVGAGVALPPVPRPPQPEPVPQATF
                     AAIADPSAAEAAPMTASDTAAPAAGDAISVEPARALRSVLLEASRRVAPGEVHYIDHP
                     LFGVVLKVEPWDPLGAANPQAFP"
     assembly_gap    8854..8953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8987..9745)
                     /locus_tag="PLW13_00035"
     CDS             complement(8987..9745)
                     /locus_tag="PLW13_00035"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HQY68782.1"
                     /translation="MQNGTRLAIIGGSAVEQLRGFATAQTVRHATPWGEPSAPVHAGA
                     FCGVPALYLARHGEPHAIAPHRINYRANLRALADLGATHVVALNTVGGIAPAAAPGSL
                     WLPHDAIDYTWGREGSFHDGVLLALDHVEFAEPFDAGMRALLGDAAVAARVSLHPRAV
                     YGCTQGPRLETAAEIARLARDGCDLVGMTALPEAALARELGLSYAMLAMVVNRAAGLS
                     PEPITLAEIRREAAACMAQVDATLESLAALLVSG"
     gene            9883..10206
                     /locus_tag="PLW13_00040"
     CDS             9883..10206
                     /locus_tag="PLW13_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68783.1"
                     /translation="MATIRPVVGNWYQETEQGSIFEVVAYDEEEQTVEIQHLDGEVEE
                     YELDTWREMTITGIEPPEDWRSGFELAQEDAELADEPMHPEDWSGALNQIEPDGIGIE
                     DDWVE"
     gene            10289..10885
                     /gene="lexA"
                     /locus_tag="PLW13_00045"
     CDS             10289..10885
                     /gene="lexA"
                     /locus_tag="PLW13_00045"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQY68784.1"
                     /translation="MSNLTERQSQVLDLIRHYIEDTGYPPTRADIARELGFRSANAAE
                     EHLRALARKGAIEMTAGASRGIRLSGESGIPVVGRVAAGSPLLAAEHIEQHVQVPARL
                     FSPAADYFLRVSGMSMKDAGILDGDLLAVHRTVEAREGQIVVARIDDEVTVKRLHRTR
                     DRHVVELLPENPDFSPIRVDLREHEFTLEGISVGVLRL"
     gene            complement(10913..11167)
                     /locus_tag="PLW13_00050"
     CDS             complement(10913..11167)
                     /locus_tag="PLW13_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68785.1"
                     /translation="MAASLLEIVELASGEVVLRRPGGEGEPLVRIHFSADSREYLSDA
                     RLEIARAMIEAGIQTAAHLAGGEAELEFIGAREDGERILH"
     gene            complement(11272..13905)
                     /gene="topA"
                     /locus_tag="PLW13_00055"
     CDS             complement(11272..13905)
                     /gene="topA"
                     /locus_tag="PLW13_00055"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQY68786.1"
                     /translation="MGKALVIVESPAKAKTINKYLGRDFIVKSSVGHIRDLPTSGNAK
                     MTDASERARSAAATRKMSEKEKAKHREKKGREQLVKRMGVDPGHGWRASYEILPGKEK
                     VVADLRKAASTADEIYLATDLDREGEAIAWHLREAIGGDPAKYRRVVFNEITRSAIKE
                     AFERPSQVDMDRVNAQQARRFLDRVVGYMLSPLLWEKVARGLSAGRVQSVAVKLLVER
                     EREIRAFVPEEFWELHADTLTAAKEALRLEVRREGEQNFRPGNREEAERAVRALEKAR
                     FVVRAREDKPTRTRPSAPFITSTLQQAVSTRLGFSVKKTMMLAQRLYEAGFITYMRTD
                     STNLSAEAVAACRDYIAATHGERYVPEKPGVYSSKAGAQEAHEAIRPSDVTLLPDALE
                     GVERDAVRVYELIWRQFIACQMPAAEYLSTSLEVAAGDFELRARGRILKFDGFLRVLP
                     AGQRKDEDVELPDVAVGAELTLQQLVPSQHFTKAPPRFTEATLVRELEKRGIGRPSTY
                     AAIISTIQDRGYARVESRRFYAEKLGDIVTDRLAEKFTNLMDYGFTASMEESLDEVAS
                     GERNWIKVLDDFYADFSTRLDAAKAADGGMRGNTPVGTDIACPACGRPMQIRTGSTGV
                     FLGCSGYSLPPAERCKETINLVHGDEVVHDEADEDEEAESRVLRTRHRCGICGTAMDS
                     YLVDAQRKLHVCGNNPDCPGYEIETGRFKLKGYEGPLIPCDKCGADMQLKTGRFGKYF
                     GCTASDCGNTRKLLRNGQPAPPKMPPVPMPHLKCQKVEDFYVLRDGASGLFLAASRFP
                     KNRETRAPLVEELLSVREQLDPKYAFLLDAPVRDDAGNPVQIRYSRKMRRQYVMSEVD
                     GKPTGWRAYERDGKWVVSKDD"
     gene            complement(13985..14485)
                     /locus_tag="PLW13_00060"
     CDS             complement(13985..14485)
                     /locus_tag="PLW13_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68787.1"
                     /translation="MMTPFLIVIGVAALFVLGPVLAMRPSPRQAQLARLRARAVAAGL
                     RVRVEAGRSRESVVDYVLPWRLDDLPQVRGLRLLLRRATDGSWEDAEFLAAPAASLGE
                     VCAAVPAGVSGLRSVGEGLAAQWNEKGRDDDVERIRDALLGLREAVIARAAQEEAARG
                     ARDIRT"
     gene            14596..15039
                     /locus_tag="PLW13_00065"
     CDS             14596..15039
                     /locus_tag="PLW13_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY68788.1"
                     /translation="MSAYRHILLGLDLSVESHQVVDRAAALRNAFGSELSVIHVIEPL
                     SFAYGGDIPMDFSGIQEEIHKQARTHMTQLCDPLNIPEKNRHLVVGRPETEIHHVAEE
                     IGADLVVVGSHGRHGLSLLMGSTANGVLHGAKCDVLAVRVKSQEK"
     gene            15103..17046
                     /locus_tag="PLW13_00070"
     CDS             15103..17046
                     /locus_tag="PLW13_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY68789.1"
                     /translation="MRFLILSVALLLAPAGAFAGNGTDLLAAQRQHYRAAKAALERGD
                     PQPLLRARTELADYPLYPYLELAQLRRRLPAMPQAEVEHFLERHGNGLPAARLRAAWL
                     PLLASHERWPEFLRHYVEDPANTERRCDHAWALLQTGASVEADRRTAQIWATPRSLPR
                     ACDRLFQAWYARGNPAPALAWQRFDAAMAAGQTGLARYVMRFLDAPARADAELFLRVA
                     ETPALVQQPARFAGANPRHAGIVAFGLSRLAARDAHAAREAFEQYARAGQIDPAALGN
                     AAPRIASALAAVSPRTAMHWIMGLDARMRSDALGEDAVRHALRGEGWDDVLATLDLLP
                     APLAATERWQYWAARSRDGAGAQAAWTQLGAVRSYYGFLAADRSGRPYAMQHQPLPVS
                     DAQLRAVERDPGIRRAREFQSIGDTLESRREWLHTIGAMNPSERLAAAAVAHRWGWHQ
                     LAISTLIAAGEWNALEMRFPTVYAQDFERAARQQRVGQDWLYAIARQESALNATVRSP
                     AGALGLMQVMPATAELTARKAGIPYRGSADLLDPATNVVIGSRYMRMMLDEFQHNRIL
                     AAAAYNAGPGRVKQWLRRLPATVEHDRFVEAIPFRETRQYVQNVLSFAVIYAYLDGRQ
                     VPVVQPAEQLIRNPWPLQAANAR"
     gene            17117..18352
                     /locus_tag="PLW13_00075"
     CDS             17117..18352
                     /locus_tag="PLW13_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQY68790.1"
                     /translation="MPDQPILLARQPIYDASLNVVAFELLYRAISPDSAEMRNGDEAS
                     SRVLLSAFGGTGIMDITDGKPAYINFTKHLIINPPPVDPQHLVIEVLEDVLVTPELLD
                     SVRRLRAQGYRIALDDFQHHPSLEPLVELADIVKLDVLELGEVKLRAEVRQLRNYPVR
                     LLAEKIESWEMYNQCVDLGFSFFQGFFLARPQLLHGRRSAPARPAIAALMAQLADPDI
                     GIPELTTTVSADPVLSFNLIRLVNSPMYRRAQPIESVREAIFALGVERVRGWCYLVAL
                     AGIGGKPSELTRIAMRRARFCELLGDIATGAVPGRCFTAGILSTLDAFMDAELGELLA
                     SLALSGEMHAALLAREGCMGVVLDTALAFETGSWERVPWSILAAHGIDQLAAERAYLD
                     SLHWVSETLGAIDAGSVFS"
     gene            complement(18345..18611)
                     /locus_tag="PLW13_00080"
     CDS             complement(18345..18611)
                     /locus_tag="PLW13_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQY68791.1"
                     /translation="MTSERGGRELVLYHTAGCHLCELAEAIALPLVAAAGVRLRLVDI
                     ADSDALMERYGTRIPVLANAALLGEEIGWPFDEAAVRGLLAALS"
     gene            complement(18601..19836)
                     /locus_tag="PLW13_00085"
     CDS             complement(18601..19836)
                     /locus_tag="PLW13_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQY68792.1"
                     /translation="MAQDGGPDGANTAWILTSTALVLFMTLPGLALFYGGLVRSKNVL
                     TVLMQCFSIACLVSVLWYALGYSIAFGTANAWFGGFDRAFLGGVAESSQSGTLPEPVF
                     LMFQMTFAIITPALIVGGFAERTRFSAVMIFSALWLLLVYAPVAHWVWGGGWLQQMGL
                     LDFAGGTVVHITAGVAALVAALVIGNRNGFGVTAMPPHNMTMTVTGAGMLWVGWFGFN
                     AGSALAANGDAGMAMLVTHLSAAAGALTWLGIEWVRFGKPSALGAVTGLVAGLGTITP
                     ASGFVGPAAALVIGTTAGTVCFFATQWLKRVLKIDDSLDVFPVHGVGGILGTLAAGIF
                     ASSELGFFSGQGLAAGRGIGAQLLIQACGVAAVGLYTALMTWLLLRVAGALVGLRVSA
                     EEETEGLDVVLHNERGYDL"
     gene            complement(20115..20930)
                     /locus_tag="PLW13_00090"
     CDS             complement(20115..20930)
                     /locus_tag="PLW13_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQY68793.1"
                     /translation="MDLPRFDTLALELADHVALVELNRPDKANAMNAALWRELEECFR
                     WCDREPAVRAVVLAARGRHFCAGIDLQMFATLDDQSLEPGRRAEHRRHHIERLQANLT
                     AIEQCRKPVLAAVQYTCIGGGVDMISCCDMRYCTADAYFAIKEIELGMVADVGTLQRL
                     PRLIGDGLMRELAYTGRRMDAAEAQRHGLVNQVHATREGMLAAVMDIARTIARRSPLA
                     IRGTKEVLLYGRDHSVADGLRYIANWNAGMLSADDLTRSVMAGGKTDELSYGD"
     gene            complement(20965..21525)
                     /locus_tag="PLW13_00095"
     CDS             complement(20965..21525)
                     /locus_tag="PLW13_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HQY68794.1"
                     /translation="MDAIAALLSRNSAARLAEPAPAGAAREQIFRAALRAPDHARLQP
                     WHFLVVEGQGREALGAAMARAMQRHAPDSGAEAQDKLRANPLRAPLVLVLVARVRDNP
                     KVPAIEQELAVACAAQNMLVAAHALGFGGIWRTGPVTYTDELREELGLAASDRVIGFL
                     YLGTPEGTPKPLPALDPAAHFHRWPR"
     gene            complement(21601..23460)
                     /locus_tag="PLW13_00100"
     CDS             complement(21601..23460)
                     /locus_tag="PLW13_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HQY68795.1"
                     /translation="MSRLPVIAGFGGISPAGRSSAHHGFRRLVIDALPAPLADATWRS
                     LGALMNVPDSDSAAAREHMRANTLVRRIGAEHFDSDGIPWHRRMTWSPAEGGMRVSIG
                     IGQLPDPLPPGWRVLATQGRTAEVLIEGPTEVLVPAARRSEVNAAGQLPTGFDPRALY
                     PARSHPRGLQMTVYGASDALHSLGIDWDLIRARVRPDQVSVYAGSGMSQLDGHGNGGM
                     MASRLMGKRVTSKQCPFGFAEMPADFINAYILGSLGNTGTSMGACASFLYNLAHGVAD
                     IRSGRARVAMVGNAEAPITPEVIEGYAAMGALATDEGLRALDGLAPGVAPDHTRACRP
                     FADNCGFTIAESAQFVVLMDAELALELGATIHAAVAEVFVNADGYKKSISSPGVGNYL
                     TMARAAALARAIVGEESLRRRSFVQAHGTGTPQNRVTESHILDETARVFGIRDWPVCA
                     LKSYLGHSIGAAAADQLVMTLGTWAHGLLPGILTAERDADDVHRTHLDILRRHRERDA
                     RELDVAIINAKGFGGNNASCTVLAPHVAARMLEKRFGRERMCEHAALNERVRERSADY
                     DARASSGQAAPIYQFDHGVLHGEDLEWDASRLRVPGYAQAIDLEMQSPYADMH"
     gene            23618..24616
                     /locus_tag="PLW13_00105"
     CDS             23618..24616
                     /locus_tag="PLW13_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY68796.1"
                     /translation="MIRVIVLAIEGALASSLVLPVEMLRAAAQHARAHSRRAPGLEVR
                     VAAEQREVLTMSGVIGLQADLAADAVQEAELIIVPSLWRAPAATVERHPRIGRALRRA
                     ARSGSRLCSVGTGSYFPAAAGLLDGRQATTHWSFFEDFARRFPAVQLQRRHLITRSGP
                     FYCAASVNSAADLTLHFIGEFFGAAAARQVESQFSPEIRRPFGAQGLGEGDAGAHPDE
                     AIRMAQDWLLANPGARLRVPELAARCGLGTRTFNRRFRAAVGTTPLAFVRTARLELAR
                     DLLRQSNLGIGEIAHRCGYADPGHFSRAFSGATGSSPQEYRARARGKLFRGQPGSP"
     gene            24741..25922
                     /locus_tag="PLW13_00110"
     CDS             24741..25922
                     /locus_tag="PLW13_00110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY68797.1"
                     /translation="MDDSTVVIAGAARTAMGGMNGALAGMAATQLGAEAIRAALARSG
                     IAPEAVNEVIMGCVLPAGLKQAPARQAALGAGIPKSAGCTTINKVCGSGMQAVMYAHD
                     QLKAGTNAIMVAGGMESMSNAPHMLLGARQGIRLGHAELKDHMFLDGLEDAYTARAMG
                     SFAQETANERGITREQMDAYAIQSLTRALAAIEQGRFKDEIVPVTVKGRKGDTVVDTD
                     EQPLSARPDKIPQLKAAFAKDGTITAANSSSISDGGSALVLTTAAEAAKRGLQPMARV
                     VAHSRHSHEPNLFTTAPVFAIQKLLAKTGWSASDVDLFEINEAFAMVAMIAIHDLGLD
                     PAKVNVHGGACALGHPLGSSGSRIIVTLLHALKHYGKKRGIASLCIGGGEATAVAVEM
                     L"
     gene            26045..27760
                     /locus_tag="PLW13_00115"
     CDS             26045..27760
                     /locus_tag="PLW13_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY68798.1"
                     /translation="MTAGGNWDHAVDVLVVGSGNGGMTAALCAHEMGAGKVLLIEKHD
                     KFGGTSAISGGGVWVPGNRYARAAGASDSREDALRYLEATVPAESVPREMLETYVDNA
                     PRMLDFLHERTRVRYVTLPQYPDYYSMLPGARTGHRSMEPEPLMKSELGDEAANLVDT
                     HHMMWMFNRFAITQAEAHDFTAQLPGWWKRAAMLVIRSVLDLPWLLKWGRSRRICTGC
                     AGIARLRLSMMERNLPLWLRTQLVELLTDAEGRVTGAVIEREGKRLRVQARKGVVLAA
                     GGFEHNQQMREQYLPRPTEARWSGGIRTNTGDAIREGMRLGAATRLMGDAWWCTTITV
                     PGEPSPRLSIMEKSYPGSCVVNAAGKRFTNESQNYMAYQKALFATHSNENPCVPSYHV
                     FDARFRRSYIVGPLYNSRMRPDWALPQRWFEAGFVHKADTIAELARKAGIDPAGLEET
                     IRRMNEFARSGKDLDFQRGDADYDRYYGDPRVTPNPCLAPIDEAPFYAMPIDPGDFGT
                     QGGLVTNTSAQVLREDGSAIDGLYAVGNCSAPVLPTYPGPGSTLGPAMTFAYQAAKHI
                     TGYRA"
     gene            27827..28765
                     /locus_tag="PLW13_00120"
     CDS             27827..28765
                     /locus_tag="PLW13_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQY68799.1"
                     /translation="MANLLHQLTLSAPLFLLVLAGYGLIRVGRWDDAIGDALGRFVFA
                     AALPAMLFRLMSGLSTLPPVDARLLVAFFGSCLVVFALGRLVAWKLFALDGVSQSVFA
                     LGGVFSNNVLLGLPIARLLLGEAALPAVALVLVFNSLILWTLVTVSVEWARHGELSAR
                     GFGATLKSVLTNPVVVAILSGTVFGMSGVALPAVLDTTLGMVAGMAAPLALIALGMGI
                     SRYRVREGLPLASAIIVIKLLVQPLVVWAIARAIGLPPLETQVVVLLASIAVGVNVYL
                     MASQFQVLQGAVAASIVLSTLASSITTPLLLALLSP"
     gene            complement(28864..30462)
                     /locus_tag="PLW13_00125"
     CDS             complement(28864..30462)
                     /locus_tag="PLW13_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY68800.1"
                     /translation="MSKDRRNVCPGIRGWIRALSIIALPLLGACAAQRTPPAPVCTGT
                     ETPASRLFLQQVSATGAIVRWHGEADVICAGTEMERLDIRVSASEDAGHRQAVLIGLQ
                     PDTVYYYSLGGAASAPAGQWFRTAPRAGQVPADGNTHIWILGDSGTETEEFMGRASHR
                     GEARAVMEGYLKYSREQAGDEPLDLLLLLGDNAYLAGTDAQWQGAFFDVYGSLLHRVA
                     TWPTIGNHEMGVTNVDVCTLFPVPGCDKGPVPMPLGGSSESADPRSYDSDGDGPDAEG
                     FPYLNIFALPAAGETGGVPSGTEQYYSFDHGNVHVVSLDSQISNRDPAQRAAMRAWLV
                     DDLSANRLDWTVVVFHHPPYSKGMNHDSDVEQAEIDMRETFGPVFQAHGVDAVYSGHA
                     HSYERSWYLHGHFGMADTFDAAQHAALDAAGAPALGQGEAPYRQLSPASGADDRVVYT
                     VAGSAGKADTAKPCRPGQRMMCSDETWLAHPAHRTFDGEEPGHRPHGLALKGSVVLDA
                     GKSTLRSRFVDEHGQVLDEFTITR"
     gene            complement(30534..30983)
                     /locus_tag="PLW13_00130"
     CDS             complement(30534..30983)
                     /locus_tag="PLW13_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY68801.1"
                     /translation="MEEVSMLAVKELTRHYEALCEQVPLRPIRTRTHYRQAVRALNEL
                     LDAGAGDEYHPLADLVATLGELIGDYEDAHRLPEGSDPVGVLRFLMQQHGLTQSGLPE
                     IGSQGVVSEVLSGRRALNVRQIDALARRFAVSPAVFMPRAPAAQGRR"
     gene            complement(30971..31276)
                     /locus_tag="PLW13_00135"
     CDS             complement(30971..31276)
                     /locus_tag="PLW13_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HQY68802.1"
                     /translation="MPLIGDTGMRVISNKALTDFATLHPASADPLRTWRHIVEASHLR
                     NFADLRRSFNSVDRVGDFYVFDIAGNRYRLIAAIHFDRQLLFIRHVFTHAQYDKWRK"
     gene            complement(31409..32014)
                     /locus_tag="PLW13_00140"
     CDS             complement(31409..32014)
                     /locus_tag="PLW13_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY68803.1"
                     /translation="MDSASQSAGGVRDRLLAAAAEVFVAQGFRDARLARVAKLAGVSR
                     STLYEHFPGKEQLLLALNHQLIDALLAQMRATLGTAPTAREGIRDWLLTGIRPGERHR
                     TLLKIIYADAVQPSLLLDRAAAAQSIRDAQAWVREALARGIAAGEFRADMDVARTAHS
                     LQNIHSLLTRQAVADYPLFEVGADEGASILELLMRGLGARD"
     gene            32113..33009
                     /locus_tag="PLW13_00145"
     CDS             32113..33009
                     /locus_tag="PLW13_00145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY68804.1"
                     /translation="MKISITITSEYNRLFGHDFTRLMDVGRWAEAAGIDQLDIAEHLL
                     MGDGKSYPFGEYPAPLDDPWPEPIAALAAIAAVTQTVRLGTGVLIAPLRPPALLAKQL
                     ATLDRISNGRLDIGLAAGWQREEYQACAIPFAQRNQRMDDTVRACRALWTGERVTLKL
                     PTVELDNVLAVPAPVQQPLPIWYGGAPTPATAQRIAEFGQGWVPLFLPEDALATGIAL
                     IGEAFAARGRDPATLQVRHQLFAQFDANGRLDLDATFAPVERLARIGVTMVSLGLGWS
                     IRDPADVEATIAAIGAWGRKLR"
     gene            33059..33829
                     /locus_tag="PLW13_00150"
     CDS             33059..33829
                     /locus_tag="PLW13_00150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY68805.1"
                     /translation="MSNTLDGKSALVTGGAGGFGKACALALARDGAAVTLMGRNAASL
                     AQARAWLLAQAPDARIAVHAGDATADADVQAALASAVAHGGGLDIVVATVGGGGYRTV
                     LEQELATFMADMQLNVGSAFAAVHHGAPLMKDGGAFVFISSTAAVLSFQGLSSYCAGK
                     AALDHFVRVAADELGSRGIRLNCVRPGLTHTDGMDPFFANPAYVESFKPLIPLGRTGM
                     PVDVAAAVRFLAGPESSWITGQSFAADGGNELRGAPRG"
     gene            complement(33859..34500)
                     /locus_tag="PLW13_00155"
     CDS             complement(33859..34500)
                     /locus_tag="PLW13_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY68806.1"
                     /translation="MNVQQALAARISANKFDTTHELAPEEVEALVAAAMEAPSAYNIQ
                     HARFLVVRDPAARAALQQVAYGQQKVRDAAATFVVLGDTRGAERLPLIAERSREAGLL
                     DAPGAAGFAASGQGAYEGKPAFAREEAIRSASMAAMALMLAATDRGLASGPMIGFDPE
                     ALRREFHIDERYLPVMMIAVGRAAPGNWPRKPRLQPGEVLVDDARPGLAHAFS"
     gene            complement(34527..35165)
                     /locus_tag="PLW13_00160"
     CDS             complement(34527..35165)
                     /locus_tag="PLW13_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HQY68807.1"
                     /translation="MFDGGFEHRDHLGHREALRDGGAQWMSTGRGVIHSEMPLAQEGR
                     LHGFQLWVNLPAAQKMKAPEYAQADAAALPQRELPGGARAKVFGGRWQLEGEEVASPL
                     DRLAAGARVLELVLPAGSATTLPVPPAESVLVYVIDGALAGEQPVQAGQTAIFGAGDA
                     LALHAGQAGARLLVLAGRPLGEPIAHYGPFVMNTEDEIRQAIADYRAGTLAQ"
     gene            complement(35165..35368)
                     /locus_tag="PLW13_00165"
     CDS             complement(35165..35368)
                     /locus_tag="PLW13_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68808.1"
                     /translation="MNTRRIDKILDSRPTSDGDGVKIRRTLGLHRHDLDPFLMIDEIR
                     ADEAADYMGGFPPHPHRASRPSR"
     gene            35467..36378
                     /locus_tag="PLW13_00170"
     CDS             35467..36378
                     /locus_tag="PLW13_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY68809.1"
                     /translation="MTTATLEQWRLLAAVVDHGGFAQAAEQLHKSQSTVSHGVKQLQQ
                     ALGLPLLTLAGRKAVLTPGGETLLRRARYLLEEAAALQHLADTLAQGWAARVSLSVDA
                     IFPQEALFEALAAFGRESPDTRVEVQESVLSGGTEALLQRQAELVITGRVPPGFLGEP
                     LLRLGFIAVAHPAHPLHQIGRALRLEDLRAHQQVVIRDSGVRLRADSGWLGAERRLTV
                     SHPATSIQALCRGLGFAWVPREKIRAELDSGQLKALPLVEGATRWAELYLVLAEREAT
                     GPAARRLAELLREAAARCRAHADGGAN"
     gene            36454..37521
                     /locus_tag="PLW13_00175"
     CDS             36454..37521
                     /locus_tag="PLW13_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQY68810.1"
                     /translation="MDAVLLAGGFVVLVLGAELLIRGSSRLAAAAGMPSLVIGLTVVA
                     FGTSAPELAVSVGSTLGGSPDIALGNVVGSNIFNVLLILGLSALIAPLTVSAQLVRFD
                     VPVLIGVSLLVWLMALDHAIGRLEGAVLGLMLAIYLDVQIRMGRRSDGRDVPVVPAAR
                     GRAAFAANVLLIVAGLVLLVAGARWLLAGATGIARAAGVSELVIGLTIVAAGTSLPEL
                     ATSVLAALRGERDIAVGNVIGSCMFNLLAVLGLTAALAPTGIRVAGAALAFDIPVMAA
                     VTLACLPVFFRRQLIARWEGGVFTLYYVAYALYLGLAASGHQRLDGYREAMLGFVLPL
                     TVLTFALIAWRSWVKDGAPSR"
     gene            37608..38669
                     /locus_tag="PLW13_00180"
     CDS             37608..38669
                     /locus_tag="PLW13_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQY68811.1"
                     /translation="MSNVLLLIGGFVVLVLGAEILVRGASRLAAAAGMPPLIIGLTVV
                     AFGTSAPEMAVSVGSALGGSPDIALGNVVGSNIFNVLLILGLSALVAPLAVSAQLVRF
                     DVPVMIAASLLVWLMALDQSISRLEGLVLALMIAIYIDMQIRMGRRGDDGDVPAVPEA
                     RGAGAMALNAVLIVAGLVLLVAGSRWLLAGATGIARGFGVSELLIGLTIVAAGTSLPE
                     LATSVLAALRGERDIAVGNIVGSNIFNIFAVLGFSAALSPAGIHVAEAALVFDIPVMT
                     AVALACLPVFFRRHLIARWEGGVFALYYIAYTLYLVLAATAHQRLDDYREAMLGFVLP
                     LTLLTLVIILWRTWVGRKA"
     gene            complement(38769..39533)
                     /locus_tag="PLW13_00185"
     CDS             complement(38769..39533)
                     /locus_tag="PLW13_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY68812.1"
                     /translation="MSDTILKELDDGVLKLTLNRPERKNAFNFEQWAAFADAIAEAQG
                     DKRVAVVLVSGAGGNFSSGTDLYEFSDAGADHPFARCARLLCDFDKPLVAAANGIAVG
                     GGATLLFHCDVLYVGESLRMRLPFVSLGLVPEFASSYQLQANIGARRAAELLFTAEWI
                     TATRALETGIATAVLPDAELFAHALAKAREIAQWPTSALQETKRTLKAVHRAGLHAAL
                     AAEDAGMVRSVGTPENREAIEAFIAKRPPDFRQFRR"
     gene            complement(39566..40267)
                     /locus_tag="PLW13_00190"
     CDS             complement(39566..40267)
                     /locus_tag="PLW13_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQY68813.1"
                     /translation="MTTIAAAVTAKDDSLSFDAAVLARRSVRAFRPDPVPQPLIEHVF
                     ALANRAPSNCNTQPWLAAVASGAACERLRQRFMADFASGQVSMDFPYAGKYAGVYKER
                     QYDAAEKLYGAMGIAREDKAARGAAFMRNFAFFDAPHVAFLFIPDWAGVREAADAGMY
                     AQTLMLALAAHGLGSCPQTALSFLCDQVRAECGVDASHRLLFGISFGYEDTANAANDC
                     RLGRAALADNVRFVS"
     gene            complement(40300..41118)
                     /locus_tag="PLW13_00195"
     CDS             complement(40300..41118)
                     /locus_tag="PLW13_00195"
                     /inference="COORDINATES: protein motif:HMM:NF005864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQY68814.1"
                     /translation="MEQLSIGTGTCLVERDGHTLVVTLNRPEAKNAFNPPMLVGMYRA
                     WRLLDSDDSLRCAVLTARGDTFCAGMDLKTGPSGGGAEADEIQALMKDVPNLHWQALL
                     RDNRPLKPIVLAVEGYALAGGTEILQGTDIRVAAEDAVFGVTEVARGLYPMSGSTIRL
                     RRQIPYCLAAEILLTGRHVTAQEALEWGLINRIVPKGKTLDEALAIAAQIGRNGPVAV
                     KAVMKSLREHEECLAEADAMLRSDEVGWPVFATEDAREGMKAFKEKRPPEFKGK"
     gene            complement(41196..42827)
                     /locus_tag="PLW13_00200"
     CDS             complement(41196..42827)
                     /locus_tag="PLW13_00200"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HQY68815.1"
                     /translation="MPARHFNLADLFEIVADTVPEREALVCGDARLTYAQLEERANRL
                     AHYLAAQGIGAGDKVGLYLYNCNEYLEGMLACFKVRAVPVNVNFRYVDEELSYILNNA
                     EMAGCIHHREFVPHLAAVRAAAPKLKTLIAVDDGTALDPATIGAIGYDAALAQGSPAR
                     DFAERSDDDLFLLYTGGTTGMPKGVMWPHKAVFYAAMGGGGHFHPAGPIEQPEDIAVR
                     AKDGFVVTGMALAPLMHGACWWYACIQLLAGGKLVLNPSHHLDGEEVWDIVARERINA
                     VSIVGDAMAIPLLDVLAQNPGRWDLSCVFNVGSGGAVFSESKQAKFREHFPNVLITNS
                     FGSSEGGQMGMDNGQRRADAENGLGNVTRTPYMDVIVEEELRHAQPGEMGIFARSGHI
                     PVGYFNDPVKTAKTFVTVEDKRWLLTGDSARLEMDGSITVFGRGSNCINSGGEKIFPE
                     EVEQALKNHPAVFDALVVATPDERWGQKVTAVISPRGAAPTLEELQQEARKHIAGYKV
                     PRELHVVGEIPRQPNGKPNYARAKEIALAGTHRAP"
     gene            complement(42937..43332)
                     /locus_tag="PLW13_00205"
     CDS             complement(42937..43332)
                     /locus_tag="PLW13_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQY68816.1"
                     /translation="MSGRASASPLPDLDSPARIRAFIEAFYARVLADERLAPIFLDVA
                     AIDLAKHLPIICSYWEKLILGSDSYRRHTMNIHRALHAKQKLEPADFERWLALFCTTL
                     DEGYSGPHAERARRVAGHIAHNMQAALVP"
     gene            complement(43310..44716)
                     /gene="der"
                     /locus_tag="PLW13_00210"
     CDS             complement(43310..44716)
                     /gene="der"
                     /locus_tag="PLW13_00210"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQY68817.1"
                     /translation="MLPVIALVGRPNVGKSTLFNRLTRSRDALVANIPGLTRDRQYGE
                     FRAGEHRFILIDTGGLSGEEAGIDAEMARQSLAAVAECDLALVLVDARAGVTPADQRI
                     VALLRESGKPFVLVVNKIDGTDELLSGAEFHELGAATMLMVSASHGRGVQQLQQALID
                     AFPDAGEPQAQEGEAGEERVRVAVVGRPNVGKSTLVNRILGEERVVVFDQPGTTRDSV
                     YVDFERGEKRYTLIDTAGLRRKRSVHETVEKFSIVKTLQAIADANVALVLVDASEGLF
                     EQDVHLIGHVIESGRSLIIVCNKWDGLGTDRKAALKSELDRRLMFADFAEVHFISALH
                     GSNVGQLYAAVDRAYASARRKLPTNRLTAILEDAVASHPPPLVHGRRIKLRMAHPGGS
                     NPPVIVVHGNQTADVPEHYRRYLENVYRRELKLVGTPLRIEFVTGTNPYAGKRNTLTP
                     RQEYKRKRMMQHVRKSKR"
     gene            complement(44728..45936)
                     /gene="bamB"
                     /locus_tag="PLW13_00215"
     CDS             complement(44728..45936)
                     /gene="bamB"
                     /locus_tag="PLW13_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HQY68818.1"
                     /translation="MMIGEKKRLGPALLRALLAASLAGMLGACGMVDYVKSIGDDEED
                     AALAPAELVEFSPEVDIRKVWSTGVGNGQGKLYNRLQPALYGDAIYAAAQNGTVVALD
                     AASGKRRWKVDVDTELSGGVGVGGDLVLVGNTRGRVIAMESATGRELWRASVSSEVLA
                     PPAADWDVVVVQTLDGKVTGLDAATGARRWTHDSSNPLLTLRGTAAPLLSEGVAYAAL
                     ASGRIVAIKADTGTVLWDGRIANPQGQSDIERTIDIEGRPLLVGANVFAVTYQGKLGG
                     LNIANGRPVWARDASSYVGIAEGFGNIYVAEASGTVSAYEAAGGALRWQNDQLARRQP
                     GTPAAIGSHVAVADFDGYVHLLSQADGHFVGRVRADGDGVRADMVTGNDMLYVYGNDG
                     GLRAYRVESR"
     gene            complement(45933..46727)
                     /locus_tag="PLW13_00220"
     CDS             complement(45933..46727)
                     /locus_tag="PLW13_00220"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY68819.1"
                     /translation="MDPYGTEEDQIKALKEWWARNGSSTLIGVGLALALVFGWQWWQQ
                     RQQGASEQAAVTYQQLLQAVDAAAADPIQGTTAAHLAAQLKELSPGSRYADYASLMLA
                     RLHVGKNELIAAEAELRALLERQPDVARGALATRIDGLLGRYADPQLGALARVRLARV
                     LLALGRSDDALAVLDAAGTDDFAVEKLELRGDILSRRNDTAAAIAAYRAAVERAGSRE
                     GATASRLLQLKLQELELGEPAAGAAPAAADPAAADATTPDTGATKQ"
     gene            complement(46765..48021)
                     /gene="hisS"
                     /locus_tag="PLW13_00225"
     CDS             complement(46765..48021)
                     /gene="hisS"
                     /locus_tag="PLW13_00225"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQY68820.1"
                     /translation="MPDILPSQTPLWQHIEHEVTSLLHAYAYREIRFPLLEPTELFQR
                     SIGEVTDIVEKEMYTFTDRNGDSLTLRPEGTAGCVRAVLQNGLLHTLPQRLWYLGPMF
                     RHERPQMGRQRQFHQIGVESFGLPGPDIEAEHLLLLARLWRRLGIDRLVTLEINSLGS
                     SVARANYREALVGYLAARAEQLDEDSRRRLGSNPLRILDTKDPGTRELLAGAPQLTDH
                     LDEESREHMRGLRELLDAAGVAYRVNPALVRGLDYYGKTVYEWVTDSLGAQGTVCAGG
                     RYDGLVEQLGGTPVPGVGFAMGLERLVLLLEVAGSQPADRAQPHAYWVVGDAAAQRFA
                     FVLAERLRERLPWLRLQMHCGEGRMKAQMKKADRSGASLVLIVGEEELAAGEVSVRGL
                     REGGPEQCRVGADALADYLAQQIVTR"
     gene            complement(48133..49257)
                     /gene="ispG"
                     /locus_tag="PLW13_00230"
                     /gene_synonym="gcpE"
     CDS             complement(48133..49257)
                     /gene="ispG"
                     /locus_tag="PLW13_00230"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQY68821.1"
                     /translation="MLHPSPIVRRRSRRIMVGSVPVGGDAPISVQSMTNTETCDVAAT
                     VGQINQLAAAGADIVRVSVPSMDAAAAFAKIRPQVGVPLVADIHFDYKIALAVAKTGV
                     DCLRINPGNIGREDRVSAVIAAARDNGIPIRIGVNAGSLEKELQRKYPEPCADAMVES
                     ALRHIDILDKHDFHDFKVSLKASDIYMTVEAYRKLAPQIEQPLHLGITEAGGLRAGTV
                     KSSVGLGMLLMDGIGDTIRISLAADPVEEVKVGWDILKSMRLRAKGINFVACPSCSRQ
                     NFDVIATMNELERRLEDVREPLDVAVIGCYVNGPGESREAHVGVTGSAPRNLVFVGGK
                     PHHKVESGSLIDDLEQTIRAALAEQAAARDSAAGSLIAKA"
     gene            complement(49271..49768)
                     /locus_tag="PLW13_00235"
     CDS             complement(49271..49768)
                     /locus_tag="PLW13_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY68822.1"
                     /translation="MAQEDTSSTDQRSPGAMLRAARESAGMSVEQVADRLHLLQSVVN
                     SLEKDCYDRIRGETFVRGYLRNYARLLGIDADEVLSRLRVTSPAGKTEKRASVAKRDR
                     WRGASGAGRVGMVLALLAVSALFLFQNREPQAAVREPGTAAVTVDTASGPQVVPLLAP
                     PRAAP"
     gene            complement(49775..50557)
                     /gene="pilW"
                     /locus_tag="PLW13_00240"
     CDS             complement(49775..50557)
                     /gene="pilW"
                     /locus_tag="PLW13_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HQY68823.1"
                     /translation="MSARGPGPWIRLLAPLVLALLVQGCVTTTNSTFKADKQAEVASR
                     VKAATDYLNQGNTEKAIVHLKRALELDPNAAPVHDTLAQVFWKTGEYELAQEHFRKAI
                     ASDPKFSRARNNYAAFLYERGDTRGAIRELEQVVADTLYESRGAAFANLGRAYQKVGE
                     TAKAEEALARAVKMDRRQWPAMLELAAINYDRGDHEQAARYYEQFRRLAPRQTPRSLL
                     LGIRLARLAGDRDAEASYSLQLKSMYPDSAEYREFSSTSSER"
     gene            complement(50554..51714)
                     /gene="rlmN"
                     /locus_tag="PLW13_00245"
     CDS             complement(50554..51714)
                     /gene="rlmN"
                     /locus_tag="PLW13_00245"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQY68824.1"
                     /translation="MENSAALIASDTPPARVNLLGMPRARMEEFLVALGEKPYRAQQL
                     FKWIYHQGVDSFEQMTDISRKLRERLAQCAEVRAPEVAEEHISADGTRKWLVRVTGGS
                     CVETVFIPEDGRGTLCVSSQVGCALDCSFCSTGKQGFQRDLTAAEIIGQVWIAARSFG
                     LPKRGGQRSITNVVMMGMGEPLLNFDNVVAAMDLMMDDFAYGISKRRVTLSTAGVVPA
                     LERLAAVSEASLAISLHAPNDALRDQLVPVNRRYPIASLLDAARGYMDAQTDSKRVIT
                     VEYTLLKGVNDSVEQARELAALLRGYPCKINLIPFNPFPQSSYERPSRNAVSRFWSVL
                     TEAGHRVTVRTTRGDDIDAACGQLVGQVQDRTRRSARHRAALACDRAPGVRA"
     gene            complement(51734..52162)
                     /gene="ndk"
                     /locus_tag="PLW13_00250"
     CDS             complement(51734..52162)
                     /gene="ndk"
                     /locus_tag="PLW13_00250"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQY68825.1"
                     /translation="MTLERTLSIIKPDAVAKNVVGEILSRFEKNGLRVVAAKMLRLTD
                     ATAGGFYAEHKGRPFYPALVEFMTSGPVLVQVLEGENAVAKNRELMGATNPKDAAPGT
                     IRADFAQSIDANAVHGSDSTASAAREIAYFFAATELCDRA"
     gene            complement(52285..53463)
                     /locus_tag="PLW13_00255"
     CDS             complement(52285..53463)
                     /locus_tag="PLW13_00255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HQY68826.1"
                     /translation="MRLPVYLDYLATTPVDPRVAACMAQCLTIDGNFGNPSSRSHIYG
                     WRAEEAVEQARREVAELVGADPREIVWTSGATEADNLALKGVAHFNRRKGRHIVTSTI
                     EHKAVLDSCRQLETEGFELSYVAPDAGGIVQPAALAPLLREDTILVSVMHVNNELGTV
                     NDIAALGALCRERGIVFHVDAAQGAGKLPIDLAALPVDLASLSAHKMYGPKGVGALYV
                     RRLPRVWLEAQMHGGGHERGMRSGTLPTHQIVGMGEAARLARAEMQAEAGRIAALRDR
                     LWASIADLGGVGQNGDPARRVAGALNVRFAGVEGESMIMAMKDIAVSSGSACTSASLE
                     PSHVLRAIGLGDELAHASLRFSLGRFSTDEDVALAADTIRRAVTRLRGSAVPEPGMLA
                     "
     gene            complement(53487..53981)
                     /locus_tag="PLW13_00260"
     CDS             complement(53487..53981)
                     /locus_tag="PLW13_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HQY68827.1"
                     /translation="MRLTTKGRYAVTAMLDLALHEGGGPVSLSDISGRQEISLSYLEQ
                     LFARLRRSGLVSSVRGPGGGYRLQRPGAEISVASIIDAVNESYEATACEGKGGCQQGE
                     ICLTHTLWNDLSEQIHSFLNGITLGELVARSDVQRVSRRQHERGTHGSRERVAGDISV
                     TALN"
     gene            complement(54036..54788)
                     /locus_tag="PLW13_00265"
     CDS             complement(54036..54788)
                     /locus_tag="PLW13_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY68828.1"
                     /translation="MLARVRVVLVGTVHSGNIGAAARAMKNMGLRRLYLAAPVEFPHR
                     EATYRAVQAVDLLESAVVTASLAEAVADCSLVIGTSARERRIQLPQLGPRELGPLCLG
                     ERRGEEIALVFGREDSGLSNEELRLCHWHVHVPTSADYSSLNLAAAVQLLCYELRMTA
                     LEQQGALTAPDWDEPLATVEGMERFYEHLDATLADIGFLNPAAPRQLMTRLRRLFNRV
                     RMDQMELNILRGILTEIGKTRAEAQRASPPNS"
     gene            54948..55757
                     /locus_tag="PLW13_00270"
     CDS             54948..55757
                     /locus_tag="PLW13_00270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQY68829.1"
                     /translation="MHPMVNIALAAARKAGEIIARAADRLDMIEVGRKSGNDFVTEID
                     KAAEREIITQLRRAYPDHLILAEESGASGDAARAGGYQWIIDPLDGTTNFMRGIPHFA
                     VSIACVRDGRLEHAVVLDPVRREEFTATRGQGAQRNGRRIRVSTRAGLKEGVLGTGIP
                     FCEPSIRHLPAYLQTLEQFASHTAGIRRAGAAALDLAYVAAGRLDGFWEMGLKPWDIA
                     AGALLVQEAGGLISDFDGGNDWMESGRIVAANPKCLKAMLQVIRPALGSAA"
     gene            complement(55776..56696)
                     /gene="secF"
                     /locus_tag="PLW13_00275"
     CDS             complement(55776..56696)
                     /gene="secF"
                     /locus_tag="PLW13_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQY68830.1"
                     /translation="MQDEQPKFIDFLKWRAWTAGLSAALCLAGLISLAVQGLNLGLDF
                     TGGTVVELGFREEVQTATVSGQLAQAGFESASVVHFGSERDVLVRLPPGDSATVGQQV
                     LAAVGGARVATLKRTDVVGAQIGDELRDQGGLALLLALALVAGYVAIRYEWKMATGAI
                     VAIFHDVLVTVGLFSIFQWEFDLAALAAVLSLVGYSINDTIVIYDRVRENFRKMRRVD
                     RFTVCNVSLTQTLGRTITTSALTQLVVVAMLVAGGPQLQGFSLAMFVGIIAGSYSTIY
                     IASAFSLWLGLDRKDLLLPAPEKLEAQETP"
     gene            complement(56710..58572)
                     /gene="secD"
                     /locus_tag="PLW13_00280"
     CDS             complement(56710..58572)
                     /gene="secD"
                     /locus_tag="PLW13_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQY68831.1"
                     /translation="MLNRYPLWKNLLVAFALLFGAIYSLPNLYPPDPAVQISGASSAL
                     EIGQPVMDKALAALEDKQIATVGSEFDSDSALLRLTGIEDQLRAQSVIKRALGDDYVV
                     ALNLAPTTPAWLAAIGGAPLKLGLDLSGGVHFLLQVDTDAAVAKRISVYESDLKNKLR
                     EARVRGLVRVVGGNRIEAAFADAATRTAAVGVIQDAMPELQRVMEERGDKFLLQLTLG
                     AQQIKEMEDYAVNQNLTTLRNRVNELGVAEPMIQKQGRNRIVVELPGVQDTAEAKRIL
                     GKTANLEFRLEARPDAASSDKEQFQFRDQRSGERSAWLERELIIGGDRVTGATSSFDE
                     NGRPQVNINLDSQGGTLMNRATRDNIKRRMAVLFLERKTRTVGYETGPDGIETPVLER
                     YDEKKIISLATIQSALGTQFRITGLDSPAESAELALLLRSGALAAPLDFVEERAVGPS
                     LGADNIRMGVQSLMIGAALVFIATLVLYRMFGVFAVVALISNVLLLVAAMSLLSATLT
                     LPGIAGIVLTVGMAVDANVIINARIREELRNGMPPHSAIGAGYDRAWLTIMDSNLTTL
                     IVGVILYAIGTGPVRGFAVTLSIGIMTSMFTAVVVTRALANLVYGGRRLTKISI"
     gene            complement(58678..62559)
                     /gene="purL"
                     /locus_tag="PLW13_00285"
                     /gene_synonym="purI"
     CDS             complement(58678..62559)
                     /gene="purL"
                     /locus_tag="PLW13_00285"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQY68832.1"
                     /translation="MLTLAGASALSPFRLRRLLGELQSIAADVRDVQARFMHFIEIDG
                     EARDDESAMLRQLLDYGPHREAQQFAGELFLVVPRPGTLSPWSSKATDIARNCGLARV
                     RRIERGIAYYVNCEGTIAAELRARIAAPLHDRMVEAVFASLEDAAKLFVHEAPRALRT
                     VDILGGGRAALERANREFGFALADDEIDYLCAAFVELGRNPADVELMMFAQANSEHCR
                     HKIFNASWSIDGEPQSHSLFGMIRNTHEQAADPAVLSAYSDNAAVIRGHEAGRFFPDP
                     ETRVFAYHPEPVHILMKVETHNHPTAIAPYPGAATGSGGEIRDEGAVGIGARPKAGLV
                     GFSVSNLRVPQFAQPWETDHGKPGRIASALEIMLEGPIGGAAFNNEFGRPAICGYFRS
                     FEEVHEGEVRGYHKPIMVAGGLGNIREGHVLKGAMGEGTRLVVLGGPAMLIGLGGGAA
                     SSMASGASHEELDFASVQRDNAEIEHRCQEVIDRCWQLGERNPIRFIHDVGAGGLSNA
                     LPELVKDGGCGGRIALEKIPSADTGLSPLELWCNEAQERYVLAVDAADLDRFAAICER
                     ERCPYAVVGEASAAAHLALTDARDGSRPVDLPLSVLFGKPPRMHREFARTVPARVPLA
                     LDHVDIADAVERVLRLPAVAGKGFLVTIGDRSVTGQVSRDQMVGPWQVPVADAGITTT
                     SFDTCAGEAMAMGERTPLALIDAAASARMAVGEALTNIASAPIARLADVKLSANWMAA
                     AGHPGEDQALFEAVRAVGMELCPALGIVIPVGKDSMSMTTRWSEAGAARAVTAPLSLI
                     VSAFAPVTDVRLAVTPEIRRDESASQLLLVDLGRGRNRLGASALAQVYKQVGDSAPDL
                     DDPALIGGFFAALQRCIAEGLLLAYHDRSDGGLFVTLCEMAFAGRAALDVDIAALGAA
                     ALAALFSEELGAVMQVRDADLAAVRAYFAAAGLGAYAHVLGRPARGDTVRIRNGERVV
                     YENSRTALHRAWAELSFRMQALRDNPDCAREEYDALLDADDPGLGARLAYDPAERIEA
                     PFIATGARPRVAVLREQGVNGQYEMAAAFDRAGFEAIDVHMSDVLSGALDLASVSGIA
                     ACGGFSYGDVLGAGGGWARTVLFNERARTQFQGFFARGDTFTLGVCNGCQMMSGLRDI
                     IPGSAHWPRFVRNASEQYEARVALVRVESSPSILLRGMEGSMIPVSVAHGEGQAEFLP
                     GALAGLEHDGRVALRYVDSRGNPTQRFPANPNGSAGAVAAVTSSDGRVTIMMPHPERV
                     FRTVQNSWRAPGWGEDGGWMRLFRNARAWLG"
     gene            62910..64328
                     /gene="mltF"
                     /locus_tag="PLW13_00290"
     CDS             62910..64328
                     /gene="mltF"
                     /locus_tag="PLW13_00290"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HQY68833.1"
                     /translation="MGILCAVLAACPSETIQSHSGPLPRDSLDEVLARGKLRVVSRVN
                     PATFVVDKHGPSGIEFELARAFAEKIGVELEMIPAANIAEVYAALDSGTADMAASGLS
                     ARLSGTSSYWYSSPYLEVRQQIVYRSGAARPRSVADLVGKRVMVLAQSSSADALAEAQ
                     RAHPGLGWMEATRIETFELLRKIMDDEIDYAIVKSNEFEMHEGLFPGIEIAFELEERE
                     QLAWATAERGRNARLHSSMQNFLARYEESGELDLLRERFFGYLPEINRKALTAFAERV
                     ESHLPRFEGVLRRIGAEEGIDWRLLAAISYQESHWNPRAVSYTGVRGMMMLTKVTARE
                     VGVTDRTNLEQSLRGGARYFLKLVEQVSEDIAPSERELFALAAYNIGPAHVEDARRIA
                     EMRGADPDVWADVEKQLPLLTKRAWHSRTRHGYARGYETLSFVNRIRQYHRFLEHHDE
                     ESNMLQVAQNARTSFAQLPTRS"
     gene            complement(64353..64820)
                     /gene="tadA"
                     /locus_tag="PLW13_00295"
     CDS             complement(64353..64820)
                     /gene="tadA"
                     /locus_tag="PLW13_00295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQY68834.1"
                     /translation="MTSAEEDEHWMRRALELARAGEGAGEVPVGAVLVRDGEVLGEGW
                     NAPIGRCDPTAHAEIVALRAAANALSNYRLSGSTLYVSIEPCAMCAGALVHARVARVV
                     FAAREPRAGAVASNLALLDAAFLNHRVAWSEGVCADDAARMMRDFFRRRRMSP"
     gene            complement(64913..65662)
                     /locus_tag="PLW13_00300"
     CDS             complement(64913..65662)
                     /locus_tag="PLW13_00300"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQY68835.1"
                     /translation="MTIWEACHGAAQIRRVAGTLYRLVESQEQVATLGYVDTLEEQAL
                     LEAMLDEVKPPYRAGTEGLHYLLKTPFRYPPLSWGSRFGRVHEPGIFYGACGTDATLA
                     EAAYYRFVFWYSMDAAPPKAAIRSQHTLFSALYRTARGVKLHEAPFARFAATIAHPKD
                     YVQTQLLGTAMREAGVEAFEYPSARDPGHGLCVGLISPAAFSEPAPRETTRWLCEAGA
                     REVAFKQVGASAVVRFVLETFLSEGELPLPA"
     gene            complement(65659..66030)
                     /locus_tag="PLW13_00305"
     CDS             complement(65659..66030)
                     /locus_tag="PLW13_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013785949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQY68836.1"
                     /translation="MSTQPHPQAAPEVVLAKAVLSAAAQLGLKQAELAAVLGMHRTAI
                     SRLKQQPALDPRSKAGELALLLVRVARALYALTGGDQDWIRHFMRSANKATGGVPARQ
                     IESIQGLVTVLQFVDALRGKV"
     gene            complement(66109..66804)
                     /locus_tag="PLW13_00310"
     CDS             complement(66109..66804)
                     /locus_tag="PLW13_00310"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HQY68837.1"
                     /translation="MRLDRFIGSRLACSARSAQHMIAAREVLLNGVPATDGSQAVSTF
                     CRVEVAGRLLRAREPVYLMLHKPRGCVSATTDRKHCTVLDLIDLPAREELHLAGRLDF
                     NTTGLLLLSSDGNWTRRITLPERKIPKTYLVHTRDPITPEYAERFAAGIYLRYENLVT
                     QPARLVTLSATRAELTIHEGRYHQVRRMFGVFRNEVLALHRLAVGGIVLDAALAPGEW
                     RHLAPAEVAAIGG"
     gene            66995..68512
                     /locus_tag="PLW13_00315"
     CDS             66995..68512
                     /locus_tag="PLW13_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HQY68838.1"
                     /translation="MPSANTSAELFRHVSAEKAGIYRSIMASFAAAKRQFRLHMRPDE
                     VLTEARWTDGAPKLEEVQAALAQLAEWGNLEAQPDTARVASISDFYRARFLYRLSHGG
                     DAVETALAAFADALERRAELQTIALEDIASRLRTLLALADAPAIDAAKLHETLRDLVR
                     VFEGLADNAQAFMAGMARSIELQQADATAVVAYKQRLIDYLERFIGDLVGRSGAIAEH
                     IAALHPRIDPLLWSAAQREARDAAPADQQAQTEALAQRQQAWRERWGGLRSWFVSAHH
                     EPPQAEVLRSKARAAIPQLLAAVAALNERRSGRSDRSADFRVLAGWFASCAGDDDSHR
                     LGRAAFALNPARHFSLNPDAGNELPATTPWAEAPPLRIHPRLREYGEAAPRGPLPRVH
                     ERSEERALLARQLREEHLQVEAARARLATGRMTRLSDIGRLDPHAFALLLSLLGEALS
                     AQSSPEDVIERQTGDGLLRIRLEPMAAHTRAEILTDAGVFAGRDHRITITATHTP"
     gene            68512..>69433
                     /locus_tag="PLW13_00320"
     CDS             68512..>69433
                     /locus_tag="PLW13_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HQY68839.1"
                     /translation="MRSDSTAIGQQQARHQREEIADALRALLMTPLMTPAHAEFAAVR
                     RHADALREWFVRETGWALHIERDAARLYKRPADLNDATRGMPGYERRRYVLLCLACAV
                     LERADPQITLHVLGDRLLALAAEPALAALGFSFTLQAQHERRELVAVCRTLLELGVLH
                     RVAGDEEGFVRGGNAGPDSLHDALYDVQRRVLAGMLAASRGPSTWPADEAPATLEARL
                     AALVTEPVPDSDEGRRTALRHHLARRLLDDPVIYLDRLDADQRAYFLNQRGTMAIRLC
                     DATGLVAEQRAEGLALADEDGELTDVAMPAE"
BASE COUNT        10426 a        24734 c        23563 g        10610 t
ORIGIN      
        1 gctgcttgtt gctggtctgc accggcttga gcgagaccgc ggcgcccgcc acgatcaccg
       61 agcagacgat gcacaggacc agggcgacca gcagggtgcg cccggtggtt tccttcctag
      121 ccgttgacat ggcgcgccag cctccggttg atgttggccc gcatcacgaa attgtcgatc
      181 agcggcgcaa agatgttggc aaaaaggatc gcgagcatca tcccctccgg atacgcagga
      241 ttcaccacgc ggatcagcat gaccatcacg ccgatcagga tgccgaaggc gagcttgccg
      301 gtgtcggtca tggcggccga taccgggtcg gtggccatga ataccgtgcc gaaagcccag
      361 ccgccgatca cgaagtgcca gtagaacggc agcgcgaaca tcgggttcgt gtccgagccc
      421 acggcattga acagcagtcc catcgccacg gtgccgatca ccacgccgag catgatccgc
      481 cacgacgcga tgccggtcca cagcagcagc accgcaccga gcaatatcgc cagtttcgag
      541 acctcgccga tgctgccggg gatctggccg aggaatgcct gcatccagga aaactgcgcg
      601 gtgattgcct cgagtccgcc attggcgccg agcgacagcg ccgtggcgcc gctcattccg
      661 tccacgccca tcgagctggc gccgctgtag ccgtctacca ccgcccagtc gaacgccgtc
      721 cacacgctgt cgcccgagat ctcggcggga taggcaaaga acaggaacgc gcgtccgacc
      781 agcgcgggat tgaggaaatt cttgccggta ccgccgaaga cttccttgcc gaccaccacg
      841 ccgaagctga tgccgagcgc gacctgccac agcgggatat cgggcggcag cgtcaacgcg
      901 aacaggatcg aggtgacgaa gaagccctcg ttgacctcgt gcccgcgcac ggcggcgaac
      961 aggatttccc acgcgatgcc cacggcaaag gtgacgatgt agatcggcag gaaatagcag
     1021 gcgccgtgcc agaagcagtc ccagatgctg ttcgggtcat agccggcggc caggccgatc
     1081 aaccagccgt gccagtcggt cttgaccagc acgccccctt cctgcatcgc gctgttggcc
     1141 tggaatccca ggttgtacat gccccagaac atcgccggca acgccgcgag ccacaccgtg
     1201 atcatcacgc gcttgagatc gatgccgtcg cgtacgtggg cattggcgcg cgtgacgctg
     1261 gccggggcgt agaagaaggt atcgagcgcc tcgtacagcg gatacagcga gtggaatcgc
     1321 ccgcccttct cgaaatgcgg ctcgacgcgg tcgagcattc gccgcaatgc tttcatgtca
     1381 ggcctccgat tcgatgcggg tgaggttgtc gcgcagaatg gggccgaact cgtacttgcc
     1441 gggacagacg taactgcaca gtgcgagatc ctcctcgtcg agctccagcg cgcccagctt
     1501 gaccgcggtc tccacgtcgc cgaccaccag cgacttcagc aaaggtgtcg ccagcacgtc
     1561 gagcggcatg actttctcgt aggcgccgat cggcacgatc gcgcgcgggc tgccgttggt
     1621 cgaggtgtcc atcgcgaagc gcctgccgcc gaggaagcgc gacaggtaga tgcccatcac
     1681 cgagtgacgg tcgacgcccg gtgacatcca gcccatgaaa cgccgctcgc gcccctcggg
     1741 cagcaccgag acctgcgtgt ggtagcgccc gagaaaggcc agtgcgccgt gcgcgcggcg
     1801 cccgccgagc acactgccgc tgatgatgcg ctgctcgccc ggggcgagct cgccggcgca
     1861 gagctcctcg gtgctggcgc ccagcacggt gcgcagcagg cgcggtttgg cgataccggg
     1921 gccggcgagc gcgacgacgc gctccgtgag caggcgcccg gtagtgaaca gatgcccgat
     1981 cgcgacgaca tcctggtagc cgacggtcca gaccgcccgg tgttcgtgca ccgggtcgag
     2041 gaagtggatg tgggtgccgg cgagtccggc ggggtgcggg ccgccgaact cctcgtgcac
     2101 cacgcgcggg gcctcgatgc gcggcacgct ggccccggcg ccgctcacca cgtagacctt
     2161 gccctcggtg agcgcgctca gcagctgcac gccgtggcgg aactcctcgg cgcgcggtgc
     2221 aagaaccacc gcggggtcgg ccgcgagcgg atgggtgtcg atcgcggtga cgaagatcgc
     2281 gtgcggcacg ctggccgggg ccggcacctt gctgtacggg cgcgtgcgca gcgcggtcca
     2341 cagcccggaa gcgcacaggt tgcggcacac ccgctcgcgc gagagcgatg ccagtgccgc
     2401 tgcctcgtag cgctcgaact cccgctcctc ggtgccttcg acgtcgatca ccaccgacag
     2461 cagcacgcgc ttgggcccgc ggttgatagc ggcgatcgtg ccggccaccg gtgccgtgaa
     2521 ctgcacgccc tcgaccttgc ggtcggtgaa cagtacttgt cccttggcta cacgatcgcc
     2581 gggctgcaca gtcatgctcg gcttcatgcc atggtaatcg ggccccagca cggccaccgt
     2641 gcgaacccgc ggtccctcat gcacgcgctg ctcgggctcc ccgctgatgg gcaggtccag
     2701 tcctcgacga atcgtgatca ttgttgctcc cgtccgacct ttccgatcaa ccggcccgcg
     2761 ccgccgctgg cagcgcgggc ctcgcctcgc gtctcatcgc gacttcggcc agctgaagcc
     2821 caccgttgtc gcgtccggat agaccgcgta gtgcacgccg gcgacgtgtt cgagcacgcg
     2881 gtgcacctgg caggcgtagc cgaactcgtt gtcgtaccag aggtagagga tcgcgctgtt
     2941 gccctgcacg atcgtggcct gcgcgtcgtg gatgcaggcg gcgcgggaac cgacgaaatc
     3001 ggaggagacc acttccggcg acgaggtgta gtcgatctgg cggctgagcg gcgagtgcag
     3061 cgaggcccag cgcatgaact cgttgacttc ctcgaccgag gtcgggcgct tcagcgtgag
     3121 gtgcaggatc gccatcgaaa cgtcgggcac cggcacgcgg atcgcattgc cggtgagctt
     3181 gcccgccatc gagggcagca ccttggccac ggcgctggcc gcgccggtct cggtgagcac
     3241 catgttcagc ggcgcgctgc gcccgcggcg gttggccttg tggaaattgt cgatcaggtt
     3301 ctggtcgttg gtgtaggcgt gcacggtctc gacgtggccc gagacgatgc cgaactgctc
     3361 gtccacggcc ttcagcgccg gcgcgatcgc gttggtggtg cacgaggccg ccgtgatgat
     3421 gcggtcgtcc gcgctgatgt cggcgtggtt cacgccgtag acgatgttct tcagcgcgcc
     3481 cttgccgggc gcggtgagga tcaccttcga cacgcccctg gcctgcaggt ggcgcgacag
     3541 tccggcctcg tcgcgccatt tgccggtgtt gtcgatcacg atggcgtcgt tgatgccata
     3601 ggccgtgtag tcgatgctct cgggcgcatc ggcgtagatc agcttcactt cgttgccgtt
     3661 ggcgatgaag gagccgttct cctcgtcgac gcggatcgtg ccgttgaagg agccgtggat
     3721 cgagtcgcgt cgcagcagcg ccgcgcgctt gaccaggtcg tcgtcgctcg atttgcgcac
     3781 caccacggcg cgcaggcgca gcacgtcacc gccgccggtc ttctcgatca gcagccgcgc
     3841 catcagccgg ccgatccggc cgaagccata gagcaccacg tcgcgcggcg cggcgaccgg
     3901 cttggcggtg ctgccgatca gcccctgcag ttccttgcgc aggaaggcct cggccgagac
     3961 gccctccgtg cgcgggcccg ccgcataggc cacgctgagg cgcccgatgt cgacgtggcc
     4021 cgtgcccagc tcgaggcggc ccagcgcctc gaccagcggc caggtctcga actccgacag
     4081 ttcattgtgc tcgacctggc gcacgaagcg atgggtcttc atgatgtcga gcacagactg
     4141 cgacaccagc gagcggccgt agacgtagac cttgacgttg cgctcgcgat acagcttgcc
     4201 cacgacaggg atcatggcct cggccagcgc ttcgcgctgc ttccagtcag agaagtagtc
     4261 cgccggtttc ggtcgcttgc gatcgggcat cgtcagtggc ctcggaaatg ctgtgggacg
     4321 gttcgctgcc gggcatggaa ccttctaagt ccccccggcg gcggcgtatt atggtcagtc
     4381 gtcactgccg ccgcaagaaa gcggccggca tcccctggct ccctggctgg cacccgcatg
     4441 aacacaaccc tgttgtcgcc cccccgggcg cgtcccggcg accacctgcg ctggggcgga
     4501 ctgatacccg gcgccgacgc gctcgcgctg gcaagcgtcg cgcgcgagca ccccggcacc
     4561 atggtcgtcc tgaccgccgg cagcgcgacg gccgagcgta tcgagtacga cctgcgtttc
     4621 ttccttgccg gcgccggcac cgccaagccc gtgctgcgcc tgcccgactg ggaaaccctg
     4681 ccctacgacc gcttctcgcc gcaccaggac ctggtctcgg accgcctgct ggcgctgtcg
     4741 cgcctggctc agggcgaccc ggtggtgctg atcgttcccg tcgcgaccct gatgcagcgc
     4801 accgcgcccg tggcctacgt gagcgcctcc tcgctggcgc tggatcccgg ccagcgcctg
     4861 ggcgtcgcgc agctgcgcaa caccctggcg cgcgccggct accgcgccgt cgacaatgtc
     4921 tacgagcacg gcgagttcgc ggtgcgcggc tccatcgtgg acctgttccc gatggggcac
     4981 gacaccccgg tgcgcatcga gctgttcgac gaggagatcg agacgctgcg cagcttcgac
     5041 cccgagaccc agcgctcgct cgcgccgctc gagcgcctgc gcctgctgcc cgggcgcgag
     5101 tacccgttcg acgatgcggc gatcgcggcg ttccgccgcc gctggcacga gcgcttcgac
     5161 aacgaccacc gcgcctgccc cgactaccag gacgtcgcgc gcggcatcgc gccgggcggc
     5221 atcgagtact tcctgccgct gttcttcgac cagtgcgcca cgctgttcga ttatctgccc
     5281 gaacagagcc tgctctgcat cgaggatgcc gcgcgcgccg ggatcgacca ggtgtgggag
     5341 gcggtcaacg gccgctaccg cagtctaggc tccgatatca cccgcccgct gctcgcgccc
     5401 gccgaggtct tcctggcgcc cgatgaactg cgcgcggcgc tcaagcgcca cacccgcatc
     5461 gagttcaccg cgctggagca gcccgagcgc gccggcgcgc acaacttcgg ctcgggcgaa
     5521 ctggcgctct cgcgcgaggc cggcaagcgc gacgatccgg tcgcgcgcat cgccgcgatc
     5581 gcggccctgc cgcaggcgcg gcgcaagctg ttctgcgccg agacgccggg gcggcgcgag
     5641 atgctggtcg agttgctcgc gcgcgccggg gtgaaggcgg aggcggtggc cggctgggag
     5701 gagttcctgc gcggggaagc gccctgcgcg atcacgatcg cgccgatcgc cgagagcttc
     5761 cggctcgacg agccctcgtg cctggtggtc gcggaatcgg cgctgttcgg caagcgcgtg
     5821 atgcagcgcc gccggcgctc gcgcgccgcc gagtccgccg agacctcgat caggaacctc
     5881 gccgagctcg acatcggctc gcccgtggtg cacctcgagc acggtgtggg ccgctacctc
     5941 gggctgcaga cgctcgaggt cgacggcgag cgcgccgagt tcctgtgcat cgagtacgcc
     6001 gaggcggcaa agctgttcgt gccggtgacc gcgctcgaca tggtcagccg ctacaccggc
     6061 atcgataccg acgccgtgac gctgaaccgc ctcggcagcg atcaatgggc gcgcgcgcgg
     6121 cgcaaggcgc aggagaagat ccgcgacgtc gcggccgaac tgctcgagat ctacgcgcgg
     6181 cgcgagtcgc gccccggaca cgccttcgcg cacccgggcg cggcctacga gagcttcgcg
     6241 gcctctttcc ccttcgagga gacgcccgac cagctcggcg cgatcgaggc ggtgatgaac
     6301 gacatgtgcg cgccgcgcgc gatggaccgc ctggtctgcg gcgatgtcgg tttcggcaag
     6361 accgaggtcg cgatgcgcgc ggccttcatc gcggtgcatt cgggcaagca ggtcgcggtg
     6421 ctggtgccga ccacgctgct ggcgcagcag cacttcgaga ccttcagcga ccgcttcgcg
     6481 gacctgccgg tcaccatcga ctcgatctcg cgctttcgct cggcgagcga gcagaagcag
     6541 gtgctcgagc gcgtggccgc cggacgggtc gacatcctga tcggcaccca cggcctgctc
     6601 ggcaccgagc tgcgttaccg cgacctcggg ctgctggtga tcgacgagga gcaccgcttc
     6661 ggcgtgcgcc agaaggacca gctgaaggcg ctgcgcgcca cggtggacat cctcacgctg
     6721 acggccaccc cgatcccgcg cacgctgaac atggccgtcg cggggctgcg cgacctgtcg
     6781 atcatcagca cgccgccggc gcgccggctg gcggtgaaga ccttcgtgca acgccacgac
     6841 gaaggcgtgg taaaggatgc gctcgatcgc gagctgcgcc gcggcgggca ggtcttcttc
     6901 ctgcacaacg acgtgaagac catcgacagg cgcgccgcgg aaatcgagcg cctgataccc
     6961 gaggcgcgcg tgacggtggc gcacggccag atgcgcgagc gtgaactcga gcgcgtgatg
     7021 tcggacttct accacaagcg cttcaacgtg ctgtgctgca ccacgatcat cgagaccggc
     7081 atcgacatcc cgagcgccaa caccatcgtg atcgagcgcg ccgacaagtt cgggctggcg
     7141 caactgcacc agctgcgcgg gcgcgtggga cgctcgcacc accaggccta cgccttcctg
     7201 ctgacgccgg acgagaaatc gatgacaccg gatgcggtga agcgcctcga cgcgatcgcg
     7261 ggcaccgatt cgctgggcgc gggcttcctg ctcgcgacac acgacctcga gatccgcggc
     7321 gccggcgaac tgctcggcga ggagcagagc gggcagatcc aggccatcgg ttacagcctc
     7381 tacatggacc tgctcgcgcg cgccgtcgag gcgatgcgct ccgggcgcct cgtagagccc
     7441 gagctcgcgc tgcgcccggc gatcgagatc aacctgcgcg tgccggcgct gatccccgac
     7501 gactacctgc ccgacgtgca caaccgcctg atcatgtaca agcgcatcgc ctcggccgca
     7561 tccgacgagg aactgcaccg gctcgaggtc gagatgatcg accgcttcgg cctgattccg
     7621 ccgccgacgc gcacgctgat gcgcatcgcg cgcctgcgcg tcgcggccca gccgctgggc
     7681 atcggcaaga tcgaggcctc cgcgaacggg ggcgtggtcg agttcgcctc ccagaccagc
     7741 gtcgacccgt tgacgatcgt ggacctggtg cagcacgatc cgcgccggta ccggctcgat
     7801 ggcggcacgc ggctgcgttt catcgaggcc acggagggcg tggaggcgcg catcgcctgc
     7861 gtcgagcaat tgctggcgcg actgctgacg ccgcgcgccg gcgccacgcc cccggcatcg
     7921 caatcccgca aggcggccgc caggagagcc ggcagatgag agcacaccga gcattcgcgc
     7981 tgctggcagc gctgctgccc acaccggcgc tgctggccca ggacattccc gcgccggccg
     8041 ccgcgatggc gccgtggatc caggtcgaac tgatcgtgtt ccgcaacctc gacacaacga
     8101 gcgcggccgg cgaacagtgg ccggcacagc ccgccctcgc ctaccccgct accctgcgct
     8161 tcctgctcga tccgggcggc atgcccgcgc cggacgcgcc ggcacccgcg gcgacgatgg
     8221 cgacggcgac gccggagccg gtcacgccaa cggcgccggt tcccttcgcg ctgctcgcgt
     8281 ccccggaaag cctgctgggc gatgccgccg cgcgcctcgc cggctcgccg ggccatcgcg
     8341 tgctcgccca cctgtcctgg cgccagcccg cgccgggcca tggcgccgag cacatcctcg
     8401 tcaccggcgg caggccctgg ggcgaacaca gggaacttga gggctacgtc tcggtgtcgc
     8461 gcgcgaactt cgtgcacgtc gcgacgcacc tgtggctgaa cgattttgcc gacgcggcag
     8521 cgggccaggc gcccccggtc ggtgccggcg tggcactgcc gccagtgccc cggccgccgc
     8581 agcccgaacc ggtaccgcag gccacgttcg ccgcaatcgc cgacccctcg gccgcggagg
     8641 ccgcgccgat gacggcgagc gataccgccg cgccggctgc cggcgatgcg atcagtgtcg
     8701 aaccggcgcg cgcgctgcgc agcgtgctgc tcgaggcgag ccggcgcgtc gcgccgggcg
     8761 aggtgcacta catcgaccac ccgttgttcg gcgtggtgct gaaggtcgag ccctgggatc
     8821 cccttggcgc cgccaatccg caggcgtttc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnncccggcg cgcaaccggc cgcggcggcc ccgccctcac cctgatacga
     9001 gcagcgcggc aagggactcg agcgtcgcgt ccacctgcgc catgcaggcg gcggcctcgc
     9061 gccggatctc ggccagcgtg atcggctcgg gcgacaggcc ggccgcgcgg ttcaccacca
     9121 tcgccagcat ggcgtaagac agcccgagct cgcgcgcgag cgccgcctcg ggcagcgccg
     9181 tcatgccgac gaggtcgcag ccgtcgcgcg cgaggcgcgc gatctccgcc gccgtttcca
     9241 gccgcggccc ctgggtgcag ccgtagaccg cccgcggatg cagcgacacc cgcgccgcga
     9301 ccgcggcgtc gcccagcaac gcgcgcatgc ccgcgtcgaa gggctcggcg aattccacgt
     9361 gatccagcgc cagcagcacg ccgtcgtgaa agctgccctc gcggccccag gtgtaatcga
     9421 tggcgtcgtg cggcagccac aacgaaccgg gcgcggccgc gggcgcgatc ccgcccacgg
     9481 tgttcagcgc caccacgtgt gtcgcgccga gatccgcgag cgcgcgcaga ttggcgcggt
     9541 agttgatgcg gtgcggcgcg atcgcgtgcg gctcgccgtg acgcgcgagg tacagcgcgg
     9601 gcacgccgca aaacgcgccg gcatgcaccg gcgccgacgg ctcgccccac ggcgtggcgt
     9661 gccgcacggt ctgcgcggtg gcgaagcccc gcagttgctc caccgcgctg ccaccgatga
     9721 tcgcgagcct cgtgccgttc tgcattctgc gtgcaactcc cgtgtccgga tcccgtgcga
     9781 atcgcgcgcg gcaccgtttg atccagcgca gcattcgatg ctggacagaa taacccaaag
     9841 tggtatgttg accgcgaacg atgagttccc agggagccgg tcatggccac gatcagacca
     9901 gtcgtcggta attggtacca ggaaaccgag cagggcagca tcttcgaagt cgtggcgtac
     9961 gacgaggaag agcagacggt cgagatccag cacctcgacg gcgaggtgga ggaatacgaa
    10021 ctcgatacgt ggcgcgaaat gacgatcacc ggcatcgagc cgccggagga ctggcgcagc
    10081 ggtttcgaac tcgcgcagga agatgccgag ctcgccgacg aacccatgca cccggaggac
    10141 tggtccggcg cgctgaacca gatcgagccc gacggcatcg gcatcgagga cgactgggtc
    10201 gagtgatcga cgccgtgcgg cgccgggctt gtcctgacga gcaggctgta tatacttaca
    10261 gcacacacgc tcgaccgagg cgcaatccat gagcaatctg accgaacgcc agagccaggt
    10321 cctcgacctt atccggcact acatcgaaga caccggctat ccgcccaccc gcgccgacat
    10381 cgcgcgcgag ctcggcttcc gttccgccaa tgccgccgag gagcacctgc gcgcgctagc
    10441 gcgcaagggt gccatcgaga tgaccgcggg cgcttcgcgc ggcatacgcc tgagcggcga
    10501 gagcggcatc cccgtggtgg gccgcgtcgc ggcgggcagc ccgctgctgg cggcggagca
    10561 catcgagcag cacgtgcagg ttccggcgcg gctgttctcg ccggcggcgg attatttcct
    10621 gcgcgtcagc ggcatgagca tgaaagacgc gggcattctc gatggcgacc tgctcgcggt
    10681 gcaccgtacc gtggaggcac gcgagggcca gatcgtggtg gcgcgcatcg acgacgaggt
    10741 cacggtgaag cgcctgcacc gcacccgcga ccgtcacgtg gtggaactgc tgcccgagaa
    10801 ccccgacttc tcacccatcc gcgtcgacct gcgcgagcac gagttcacgc tcgagggcat
    10861 cagcgtcggc gtgctgcgcc tgtaaaccgg cattccgtgt gggtcggcat tcctagtgaa
    10921 ggatccgctc gccgtcctcg cgcgcgccga tgaattcgag ctcggcctcg ccgccggcga
    10981 gatgcgcggc cgtctggata ccggcctcga tcattgccct ggcaatctcc agccgcgcgt
    11041 ccgagaggta ttcgcgcgaa tcggcggaaa aatggatgcg caccagcggc tcgccctcgc
    11101 cgccgggtcg gcgcagcacg acttcgccgg aggcgagttc gacgatttcc agaagtgatg
    11161 ccgccaaacg ggcgtcctcc agcaggtgcc gggtggcgac aaccgccagc cccggatgaa
    11221 ttctagcccg tcacggcgcc gcgcgcccgc aagcgcgcag tgccgggcgc atcagtcgtc
    11281 cttgctcacc acccacttgc cgtcacgctc gtaggcacgc cagcccgtag gcttgccgtc
    11341 gacctcgctc atcacgtact ggcggcgcat cttgcggctg tagcggatct gcaccggatt
    11401 gcccgcgtcg tcgcgcaccg gcgcatcgag caggaaggcg tacttcgggt cgagctgctc
    11461 gcgcaccgac agcagttcct cgaccagcgg cgcgcgcgtc tcgcggttct tgggaaagcg
    11521 gctggcggcc aggaacaggc ccgaggcgcc gtcgcgcaac acgtagaagt cctcgacctt
    11581 ctggcacttc aggtgcggca tcggcaccgg cggcatcttg ggcggcgcgg gctgcccgtt
    11641 gcgcagcagc ttgcgcgtat tgccgcagtc gctcgcggtg cagccgaagt acttgccgaa
    11701 gcgcccggtc ttcagctgca tgtccgcgcc gcacttgtcg caggggatca gcggaccttc
    11761 atagcccttg agcttgaagc ggccggtctc gatctcgtag cccgggcagt cggggttgtt
    11821 gccgcagacg tgcagcttgc gctgcgcatc gacgaggtag ctgtccatcg cggtgccgca
    11881 gatgccgcag cgatgacgcg tgcgcagcac ccgcgattcg gcctcctcgt cctcgtcggc
    11941 ctcgtcgtgc acgacctcgt cgccgtgcac caggttgatg gtctccttgc agcgctccgc
    12001 cggtggcagc gagtagccgg agcaacccag gaacacgccg gtgctgccgg tgcggatctg
    12061 catcggccgc ccgcacgccg ggcaagcgat gtcggtgccg accggcgtat tgccgcgcat
    12121 gccgccgtcg gcggccttgg cggcgtcgag ccgcgtggag aagtcggcgt agaaatcgtc
    12181 cagcaccttg atccagttgc gctcgcccga ggcaacctcg tcgagagact cctccatgct
    12241 ggcggtgaag ccgtagtcca tcaggttggt gaacttctcg gcaaggcggt cggtcacgat
    12301 gtcgccgagt ttctcggcgt agaagcgccg gctctcgacg cgcgcatagc cgcggtcctg
    12361 gatggtggag atgatcgcgg cataggtcga gggccggccg atgccgcgct tctcgagctc
    12421 gcgcaccagc gtggcctcgg taaagcgcgg cggcgccttg gtgaagtgct ggctgggcac
    12481 gagctgttgc agtgtcagct cggcacccac cgccacgtcg ggcagttcga cgtcctcgtc
    12541 cttgcgttgt cccgcgggca gcacgcgcag gaagccgtcg aatttgagga tgcgcccgcg
    12601 cgcgcgcaac tcgaaatcgc ccgcggccac ctcgagcgag gtgctgaggt attcggccgc
    12661 cggcatctgg caggcaatga actggcgcca gatcagctcg tatacccgca ccgcgtcgcg
    12721 ctccacgccc tcgagcgcat cgggcagcag cgtcacgtcc gagggacgga tcgcctcatg
    12781 cgcttcctgc gcgccggcct tgctgctgta gacgccgggt ttttccggca cgtagcgctc
    12841 gccgtgcgtg gcggcgatgt agtcgcggca ggccgcgacg gcctcggcgc tgaggttggt
    12901 cgagtcggta cgcatgtacg tgatgaagcc ggcctcgtag aggcgctggg cgagcatcat
    12961 ggtcttcttc acgctgaacc ccaggcgcgt gctcacggcc tgctgcagcg tggaggtgat
    13021 gaacggcgcc gaggggcgcg tgcgcgtggg cttgtcctcg cgcgccctga ccacgaagcg
    13081 cgccttctcc agcgcgcgca ccgcgcgctc ggcctcctcg cggttgccgg ggcggaagtt
    13141 ctgctcgccc tcgcggcgca cctcgagacg caatgcctcc ttcgcggcgg tcagcgtatc
    13201 ggcgtgcagt tcccagaact cctcggggac gaaggcgcgg atctcgcgtt cgcgctcgac
    13261 cagcagcttc accgccaccg actgcacgcg tccggccgac aggccgcgcg cgaccttctc
    13321 ccacagcagc ggcgagagca tgtagccgac cacgcggtcg aggaagcgcc gcgcctgctg
    13381 cgcgttgacg cggtccatgt cgacctgcga cgggcgctcg aaggcttcct tgatggcgct
    13441 gcgcgtgatc tcgttgaaca cgacgcggcg gtacttggcc gggtcgccgc cgatggcctc
    13501 gcgcaggtgc cacgcgatgg cctccccctc tcggtcgagg tcggtcgcga ggtagatttc
    13561 atcggccgtg ctggcggcct tgcgcagatc ggcgaccacc ttctccttgc ccgggaggat
    13621 ctcgtaactg gcacgccagc cgtgcccggg gtccacgccc atgcgcttga ccagttgctc
    13681 gcgccccttc ttctcgcgat gcttcgcctt ctccttttcc gacatcttgc gcgtcgcggc
    13741 ggcgctgcgc gcccgctccg aggcatcggt catctttgca ttaccgctgg tcggcaggtc
    13801 acggatatgg cccacgctcg acttcacgat gaagtcgcgg ccgagatact tgttgatggt
    13861 cttcgcctta gccggcgact ccacgatgac cagtgccttg cccatgatgc tgtcggtttt
    13921 ccagggtaaa tagagaaggg aggaaaaaaa gaggcgccat atatacgggc tgccccctac
    13981 agggtcaagt gcggatgtcc cttgcgccgc gtgccgcttc ctcctgcgcg gcacgggcga
    14041 tcaccgcctc gcgcagcccg agcagggcat cgcggatgcg ctcgacatcg tcgtcgcgcc
    14101 ccttctcgtt ccactgcgcc gccagcccct ccccgacgct gcgcagcccc gagacgcccg
    14161 cgggcacggc cgcgcagacc tcgcccaggc tagcggcggg agcggccagg aactccgcat
    14221 cctcccagga tccgtcagtg gcacggcgaa gcagcaggcg caacccgcgc acctggggca
    14281 gatcgtccag gcgccacggc agcacgtagt ccacgacgga ttcgcggctg cgcccggcct
    14341 cgacgcgcac ccgcaggccg gcggccactg cgcgcgcgcg caggcgcgcg agctgggcct
    14401 ggcgcggcga aggacgcatc gcgagcacgg ggccgagcac gaacagcgcc gcgaccccga
    14461 tcacgatcag aaatggcgtc atcatcggca tgcgtctcct gcgcgccgcg ggcgcggcaa
    14521 ttgcggcgcc ggcaaatttt gagctatggt agcccaaggc attccggcag ggtcaacatc
    14581 aaggaaacac cgctgatgag cgcataccgt cacattctcc tcggccttga cctgagcgtc
    14641 gaatcgcacc aggtggtgga tcgcgccgcg gcattgcgca atgccttcgg ctccgagctc
    14701 agcgtgatcc acgtgatcga gccgctcagc ttcgcctacg gcggcgacat cccgatggac
    14761 ttctccggca tccaggagga aatccacaag caggcgcgca cgcacatgac gcagctctgc
    14821 gacccgctga acatcccgga gaagaaccgc catctcgtgg tgggccgtcc cgagaccgag
    14881 atccaccacg tggccgagga gatcggcgcc gacctcgtcg tcgtgggcag ccacggccgc
    14941 cacggcctct cgctgctgat gggctccacc gcgaacggcg tgctgcacgg cgcgaagtgc
    15001 gacgtgctcg ccgtgcgggt gaaatcgcag gaaaaatgag gcagcggcgc tgcctcgcag
    15061 cgcgatcgtc ccctcccctg gtccgcggca ccggcctgca gcatgcgttt cctgatcctg
    15121 tcggtcgcac tgttgctggc accggcgggc gcgttcgccg gcaacggcac ggacctgctc
    15181 gccgcgcagc gccagcacta ccgcgccgcc aaagccgcgc tcgagcgcgg tgacccgcag
    15241 cccctcctgc gcgcgcgcac cgagctcgcc gactacccgc tctaccccta cctcgagctc
    15301 gcgcaactgc gccgacgcct gcccgcgatg ccgcaagccg aggtggagca tttcctggag
    15361 cgccacggca acggcctgcc cgcggcgcgg ctgcgcgcgg catggttgcc gttgctcgcc
    15421 tcgcacgagc gctggccgga attcctgcgt cactacgtcg aggatccggc gaacaccgaa
    15481 cggcgctgcg atcacgcctg ggcgctgctg cagaccggcg cgagcgtcga ggccgaccgc
    15541 aggacagcgc agatctgggc aacaccgcgc tcgctgccaa gggcatgcga ccggctgttc
    15601 caggcctggt acgcgcgtgg caatcccgcg ccggcgctgg cgtggcagcg tttcgacgcc
    15661 gcgatggccg ccgggcagac cggactggcg cgctacgtca tgcgcttcct cgacgcgccg
    15721 gcgcgcgccg acgccgaact gttcctgcgc gtcgccgaga cgcccgcgct ggtgcaacag
    15781 ccggcacgct tcgccggcgc caacccgcgc cacgccggga tcgtcgcctt cgggctgtca
    15841 cgtctcgccg cgcgcgacgc gcacgccgcg cgcgaggcgt tcgagcagta cgcacgtgcc
    15901 ggacagatcg atccggccgc gctcggcaac gccgcgccgc gcatcgccag cgcgctcgcc
    15961 gcggtctcgc cacgcacggc gatgcactgg atcatggggc tcgatgcgcg catgcgcagc
    16021 gatgccctcg gcgaggacgc ggtgcgccat gcgctgcgcg gcgagggctg ggacgacgtg
    16081 ctggcaacgc tggacctgct gccggcgccg ctggccgcca ccgaacgctg gcagtactgg
    16141 gcggcgcgca gccgcgacgg cgccggcgcg caggccgcgt ggacgcaact cggcgccgtg
    16201 cgcagctact acggatttct cgccgccgat cgcagcggcc gcccctacgc gatgcagcac
    16261 cagccgctgc ccgtctccga cgcgcagctg cgcgccgtgg agcgcgatcc gggcatccgg
    16321 cgcgcgcgcg aattccagtc gatcggcgat accctcgagt cgcggcgcga atggctgcac
    16381 accatcggtg ccatgaaccc gtccgaacgc ctcgccgccg cggccgtcgc ccaccgctgg
    16441 ggctggcacc agctcgcgat cagcacgctg atcgcggccg gggaatggaa tgcgctggag
    16501 atgcgcttcc ccacggtcta cgcgcaggac ttcgagcgcg cggcgcgcca gcagcgcgtc
    16561 gggcaggact ggctctacgc gatcgcgcgc caggagagtg ccctcaacgc cacggtccgc
    16621 tccccggccg gggcgctcgg cctgatgcag gtgatgccgg caacggccga gctaaccgcg
    16681 cgcaaggccg gcatcccgta ccgcggcagc gcggacctgc tcgatcccgc caccaacgtg
    16741 gtgatcggca gccgctacat gcgcatgatg ctcgatgagt tccagcacaa ccgcatcctc
    16801 gcggccgccg cgtacaacgc cggccccggg cgcgtgaagc aatggctgcg gcgtctcccg
    16861 gcgacggtcg agcacgaccg tttcgtcgag gccatcccgt ttcgcgagac gcgccagtac
    16921 gtgcagaacg tgctgagctt cgcggtcatc tacgcctatc tcgacggccg ccaggtaccc
    16981 gtggtccagc ccgccgaaca actcatccgc aatccctggc cgctgcaggc cgcgaacgcg
    17041 cgctgacgcc cttgtcgggc gcaacccgcg gtgcaatcat gcattcgcgc catacccaac
    17101 cagagaagaa cgcgtcatgc cggatcagcc gatcctgctc gccaggcaac cgatctacga
    17161 tgcctcgctg aacgtcgtgg cattcgagct cctgtatcgc gcgatatcgc cggattcagc
    17221 ggagatgcgc aatggcgacg aggcctcctc gcgcgtgctg ctgagcgcct ttggcggcac
    17281 cggcatcatg gacatcaccg acggcaagcc ggcctacatc aatttcacca agcacctgat
    17341 catcaacccg ccgccggtgg atccgcagca cctggtgatc gaggtgctcg aggacgtcct
    17401 cgtcaccccg gagctgctgg acagcgtgcg ccggctgcgc gcgcagggtt accgcatcgc
    17461 gctggacgac ttccagcacc acccgtcgct cgagccgctg gtggaactgg ccgacatcgt
    17521 gaagctcgac gtgctcgagc tcggcgaggt gaagctgcgc gccgaggtgc gccagctgcg
    17581 caactacccc gtgcgcctgc tcgccgagaa gatcgagtcc tgggagatgt acaaccagtg
    17641 cgtcgatctg ggcttcagct ttttccaggg ctttttcctc gcgcgaccgc agctgctgca
    17701 cgggcgccgc agcgcgccgg cgcgcccggc gatcgccgca ctgatggcgc agctcgccga
    17761 tccggacatc ggcatccccg agctgacgac caccgtgtcg gccgatccgg tgctcagctt
    17821 caacctgatc cggctggtca actcgccgat gtaccggcgc gcgcagccga tcgaatcggt
    17881 gcgcgaggcg atctttgccc tgggcgtgga gcgcgtgcgc ggctggtgct acctggtggc
    17941 gctcgccggg atcggcggca agccctcgga gctcacccgc atcgcgatgc gccgggcccg
    18001 attctgcgag ctgctcggcg atatcgcgac cggcgccgtt cccggtcgct gcttcaccgc
    18061 agggatcctg tccacgctcg atgcgttcat ggacgcggaa ctcggcgaac tgctggcgag
    18121 cctggcgctc agcggcgaaa tgcacgctgc gctgctcgcg cgcgagggct gcatgggcgt
    18181 cgtgctggat acggcgctcg ccttcgagac cgggtcatgg gagcgggtgc cgtggagcat
    18241 cctcgccgcg cacggcatcg accagctcgc cgccgagcgc gcctacctgg acagcctgca
    18301 ctgggtcagt gaaacgctcg gcgccatcga cgcaggcagc gtattcagct gagcgccgcc
    18361 agcaggccgc gcaccgcggc ctcgtcgaag ggccagccga tttcctcgcc gagcagcgcc
    18421 gcattggcca gcaccgggat gcgcgtgccg tagcgttcca tcagcgcatc gctgtcggcg
    18481 atgtcgacca ggcgcaggcg cacgccggcc gccgccacca gcggcagcgc gatcgcctcg
    18541 gccagctcgc acaggtgaca gcccgcggtg tggtagagca cgagctcgcg cccgccgcgc
    18601 tcagaggtca tagccacgct cgttgtgcag cacgacgtcc agcccctcgg tctcctcctc
    18661 ggcgctgacg cgcagaccca ccagcgcgcc ggcgacgcgc agcagcagcc aggtcatcag
    18721 cgccgtgtac aggccgaccg cggcaacgcc gcaggcctgg atcagcagtt gcgcaccgat
    18781 cccgcgcccc gcggccagcc cctggccgct gaagaacccg agctcgctgc tcgcgaagat
    18841 ccccgcggcc agcgtgccga gaatgccccc gaccccgtgc accgggaaca cgtcgagcga
    18901 gtcgtcgatc ttcagcacgc gcttgagcca ctgcgtggcg aagaagcaca ccgtgcccgc
    18961 cgtggtacct atcaccagcg ccgccgcggg gcccacgaag cccgaggccg gcgtgatggt
    19021 gcccagaccc gcgaccagcc cggtcacggc gccgagggcg ctgggcttgc cgaacctgac
    19081 ccactcgatg ccgagccagg tcagcgcgcc ggccgccgcc gagaggtgcg tgaccagcat
    19141 cgccatgccc gcatcgccgt tggccgccag cgcgctgccc gcgttgaagc cgaaccagcc
    19201 gacccagagc atgccggcgc cggtgaccgt catcgtcatg ttgtgcggcg gcatggcggt
    19261 cacgccgaag ccgttgcggt tgccgatcac cagcgccgcg accagcgccg cgacgcccgc
    19321 ggtgatgtgc accacggtgc ccccggcgaa atcgagcagc cccatctgct gcagccagcc
    19381 gccgccccac acccagtgcg ccaccggggc gtacaccagc agcagccaca gcgccgagaa
    19441 gatcatcacg gccgaaaagc gcgtgcgctc ggcgaagccg ccgacgatca gcgccggcgt
    19501 gatgatcgcg aaggtcatct ggaacatcag gaacacgggc tcgggcaggg tgccgctctg
    19561 cgagctctcg gcgacgccgc cgaggaaggc ccggtcgaag ccgccgaacc aggcgttggc
    19621 cgtgccgaag gcgatgctgt aacccagcgc gtaccacagc accgagacca ggcaggcgat
    19681 cgagaagcac tgcatcagca ccgtcagcac gttcttgctg cgcaccaggc cgccgtagaa
    19741 cagcgccagc ccgggcagtg tcatgaacag caccagcgcc gtggaggtga gtatccacgc
    19801 cgtgttggcg ccgtccggcc cgccgtcctg cgccagcacc ggcgccgctc ccgcggcgag
    19861 cagcagtgcg gccgcgccgc gcatccctcg tgcaatcccg tcgactctca tcgctttctc
    19921 ccgtggtggc gaccgccttc gcattatgct ggtgcaatcg gggcgatgcg cgcacgcaaa
    19981 tggtgcgtcc gggcgccgac caaaggcatg ccccgggcca ggccccgata tcgtgcaacg
    20041 tccagcaaaa tgcacgccag aatgcgtgcc cgaaacgggg cccgcgaccg cactcggggg
    20101 cgcgcctggc gtgttcagtc gccgtaggag agctcgtcgg tcttgccgcc ggccatcacc
    20161 gaacgcgtca ggtcgtcggc gctcagcatg ccggcgttcc agttcgcgat gtagcgcagc
    20221 ccgtcggcga cactgtggtc gcggccgtag agcagcacct ccttggtgcc gcggatcgcc
    20281 agcggcgagc gccgggcgat ggtgcgcgcg atgtccatca ccgcggccag catgccctcg
    20341 cgcgtggcat ggacctggtt gaccagcccg tggcgctgcg cctccgccgc gtccatgcgc
    20401 cgcccggtat aggccagctc gcgcatcagg ccgtcgccga tcagccgcgg caggcgctgc
    20461 agcgtgccga cgtcggcgac catgccgagc tcgatctcct tgatcgcgaa atacgcgtcc
    20521 gcggtgcagt agcgcatgtc gcagcagctg atcatgtcga cgccgccgcc gatgcaggtg
    20581 tactgcaccg ccgcgagcac cggcttgcgg cactgctcga tcgccgtcag gttcgcctgc
    20641 aggcgctcga tgtggtggcg ccggtgctcg gcgcgccgcc ccggctccag cgactggtca
    20701 tcgagcgtgg cgaacatctg caggtcgatg ccggcgcaga aatgccggcc gcgcgcagcc
    20761 agcacaacgg cgcgcaccgc cggctcgcga tcgcaccagc ggaagcactc ctctagctcg
    20821 cgccacagcg cggcgttcat cgcgttggcc ttgtccgggc ggttcagctc gaccagcgcc
    20881 acgtgatcgg cgagctcgag cgccagcgtg tcgaaacggg gaagatccat gcgcgaactc
    20941 ctgttgcgtc gtcgtcgata gcgctcagcg cggccaacgg tggaaatgcg ccgccgggtc
    21001 gagcgccggc agcggcttgg gcgtgccttc cggcgtgccc aggtaaagga agccgatcac
    21061 gcgatcgctc gccgccagcc ccagctcctc gcgcagttcg tcggtgtacg tgacggggcc
    21121 ggtgcgccag atgccgccga agccgagcgc gtgcgccgcg accagcatgt tctgcgccgc
    21181 gcaggcgacc gcgagctcct gctcgatcgc gggcaccttg gggttgtcgc gcacgcgcgc
    21241 caccagcacc agcaccagcg gcgcgcgcaa cgggttcgcg cgcagcttgt cctgcgcctc
    21301 ggcgccactg tcgggcgcgt ggcgttgcat cgcgcgcgcc atcgccgcac cgagcgcctc
    21361 gcgcccctgc ccctcgacga ccaggaaatg ccaaggctgc agccgggcgt ggtccggcgc
    21421 gcgcagcgcg gcgcggaaga tctgctcgcg cgcggcgccc gcgggtgcgg gctcggccag
    21481 gcgcgcggcg gaattgcggc tcagcagtgc ggcgatagcg tccatgtcac gacctgtgcg
    21541 gcgatgtcgc gcgccacact gcaatcatcg gcgcgcgatt gcaagcgcgc cggcgatggt
    21601 tcagtgcatg tcggcgtagg gcgattgcat ctcgagatcg atcgcctgcg cgtagccggg
    21661 cacgcgcagc cggctggcgt cccactccag gtcctcgccg tgcagcacgc cgtggtcgaa
    21721 ctggtagatc ggcgcggcct ggccgctgct ggcgcgcgcg tcgtagtcgg cgctgcgctc
    21781 gcgcacgcgc tcgttcagcg ccgcgtgctc gcacatgcgc tcgcgcccga agcgtttctc
    21841 cagcatgcgc gcggcgacgt gcggcgccag caccgtgcag ctcgcgttgt tgccgccgaa
    21901 acccttggcg ttgatgatcg cgacatccag ctcgcgcgca tcgcgctcgc ggtgacggcg
    21961 caggatgtcg aggtgcgtgc ggtgcacatc atcggcgtcg cgctccgcgg tgaggatgcc
    22021 gggcagcagc ccatgggccc aggtgcccag cgtcatcacc agctggtcgg cggcggcggc
    22081 gccgatcgaa tgcccgagat agctcttcag cgcgcacacc ggccagtcgc ggatgccgaa
    22141 aacgcgcgcg gtctcgtcga ggatgtgcga ctcggtcacg cggttctgcg gcgtgccggt
    22201 gccgtgcgcc tgcacgaagg agcgccggcg cagggattcc tcgcccacga tcgcgcgcgc
    22261 cagcgcggcg gcgcgcgcca tggtcagata gttgcccaca ccgggcgagg agatcgactt
    22321 cttgtagccg tcggcgttga cgaacacctc cgccaccgcg gcgtggatgg tggcgccaag
    22381 ttccagcgcc agctccgcgt ccatcagcac cacgaactgc gccgattcgg cgatcgtgaa
    22441 gccgcagtta tcggcgaagg gccggcaggc gcgggtgtgg tccggcgcca cgccgggcgc
    22501 caggccatcg agggcgcgca gcccctcgtc ggtggcgagc gcgcccatcg cggcgtaacc
    22561 ctcgatcact tccggcgtga tcggcgcctc ggcattgccc accatggcca cgcgcgcgcg
    22621 cccggagcgg atgtcagcga cgccgtgcgc gaggttgtac aggaacgagg cgcaggcccc
    22681 catgctggtg ccggtgttgc cgaggctgcc gaggatgtag gcgttgatga agtccgccgg
    22741 catctcggcg aagccgaagg gacactgctt ggaggtcacg cgcttgccca tcaggcgcga
    22801 ggccatcatg ccgccgttgc cgtgtccgtc gagctggctc atgccgctgc cggcatagac
    22861 gctgacctgg tcgggacgca cgcgcgcgcg gatcaggtcc cagtcgatgc cgagcgagtg
    22921 cagcgcgtcg gaggcgccgt agaccgtcat ctgcaggccg cgcggatggc tgcgtgccgg
    22981 gtagagggcg cgcgggtcga atccggtggg caactgcccg gcggcattga cctcggagcg
    23041 ccgcgccgcc ggcaccagca cctcggtcgg cccctcgatc aggacctcgg ccgtgcggcc
    23101 ctgcgtggcg agcacccgcc accccggggg cagcggatcg ggcaactgcc cgatgccgat
    23161 gctcacgcgc atgccgccct cggccggcga ccaggtcatg cgccggtgcc agggaatgcc
    23221 gtcgctgtcg aaatgctccg cgccgatgcg ccgcaccagg gtgttggcgc gcatgtgctc
    23281 gcgcgccgcg gcgctgtcgc tgtcgggaac gttcatcaac gcgcccaggc tgcgccaggt
    23341 ggcatcggcc agcggcgccg gcagcgcatc gatcacgagg cggcggaagc cgtggtgggc
    23401 cgaactgcgg ccggcgggcg agatgccgcc gaatccggcg atgacgggaa gcctggacat
    23461 ggagcgtttt ccttactgcg gatggctgcg gcagccgggg tgggtggcgc gcaacggatc
    23521 gattccagtc tatggcgaag gccgcggggc ggcttgtaaa ttctggtcat cgcctttaac
    23581 tttccggcca gccaatccgg gaattttcca tccgcccatg atcagggtca tcgtgctcgc
    23641 catcgagggc gcactggcct ccagcctggt gctgccggta gagatgctgc gcgccgcggc
    23701 gcagcacgcg cgcgcgcatt cgcgccgggc gccgggcctc gaggtccgcg ttgccgccga
    23761 gcagcgcgag gtgctcacca tgtcgggggt gatcggcctg caggcggatc tcgccgccga
    23821 tgccgtgcag gaggccgaac tgatcatcgt gccctcgctg tggcgcgcgc cggcggcgac
    23881 cgtcgagcgc cacccgcgca tcgggcgcgc cctgcgccgc gccgcgcgca gcggctcgcg
    23941 gctgtgcagc gtgggcacgg gcagctattt tcccgcggcc gcgggactgc tggacggccg
    24001 ccaggccacc acgcactggt cattcttcga ggatttcgcg cggcgcttcc ccgccgtgca
    24061 actgcagcgg cgccacctga tcacgcgctc cgggccgttc tattgcgcgg ccagcgtcaa
    24121 ctcggccgcc gacctgacgc tgcacttcat cggggaattc ttcggcgcgg cggccgcgcg
    24181 ccaggtcgag agccagtttt cgccggaaat ccgccggccc ttcggcgcgc agggcctcgg
    24241 ggaaggcgat gccggcgcgc accccgacga agcgatccgc atggcgcagg actggctgct
    24301 cgccaatccc ggcgccaggc tgcgcgtacc cgagctcgcg gcgcgctgcg gtctgggcac
    24361 ccgtaccttc aaccggcgct ttcgtgccgc cgtgggcacc acgccgctcg ccttcgtgcg
    24421 cacggcccgc ctcgagctcg cgcgcgacct gctgcgccag agcaatctcg gcatcggcga
    24481 gatcgcccac cgctgcggtt atgccgatcc gggccacttc agccgcgcct tctccggcgc
    24541 caccggcagc agcccccagg agtaccgcgc ccgcgcccgc ggcaagctct ttcgcggcca
    24601 gccgggctcg ccatgacacg catttggccc gtcgaatggc catcattctt gtgtgcgctc
    24661 cggcgatatg gccaaatgcc ggccctgaat ttccccaacc cgatgcactc gcgcgaacgc
    24721 gcgctgacag gagaaaggcg atggacgact cgacggtggt gatcgcgggc gcggcgcgca
    24781 cggcaatggg cggcatgaac ggcgcgctgg ccggcatggc ggcgacgcag ctcggcgccg
    24841 aggcgatccg cgcggcgctg gcgcgcagcg gcatcgcgcc ggaagcggta aacgaggtca
    24901 tcatgggctg cgtgctgccg gccggcctga agcaggcccc ggcgcgccag gcggcgctcg
    24961 gcgccggcat acccaagagc gcgggctgca ccacgatcaa caaggtctgc ggctccggca
    25021 tgcaggcggt gatgtacgcg cacgaccagc tgaaggcagg caccaacgcc atcatggtcg
    25081 ccggcggcat ggagagcatg agcaacgcgc cgcacatgct gctcggcgcg cgccagggca
    25141 tccgtctggg ccacgccgag ctcaaggacc acatgttcct cgacgggctc gaggacgcct
    25201 acaccgcgcg cgcgatgggc tccttcgcgc aggagaccgc caacgagcgc ggcatcacgc
    25261 gcgagcagat ggacgcctac gcgatccagt cgctgacgcg cgcgctggcc gcgatcgagc
    25321 agggccgctt caaggacgag atcgtgccgg tcacggtcaa gggccgcaag ggcgacaccg
    25381 tggtcgatac cgacgagcag ccgctctcgg ccaggcccga caagatcccg caactcaagg
    25441 ccgccttcgc caaggacggc acgatcaccg cggcaaattc gagctcgatc tccgacggcg
    25501 gctccgcact ggtgctgacc accgcggccg aggcggcgaa gcgcggcctg caaccgatgg
    25561 cgcgcgtcgt ggcccacagc cgccattcgc acgagcccaa cctgttcacc accgcacccg
    25621 tgttcgcgat ccagaagctg ctcgcgaaga ccggctggag cgcaagcgac gtggacctgt
    25681 tcgagatcaa cgaggccttc gcgatggtgg ccatgatcgc gatccacgac ctcggcctgg
    25741 acccggcgaa agtgaacgtg cacggcggcg cctgcgcgct gggccacccg ctgggctcca
    25801 gcggcagccg catcatcgtc accctgctgc acgcgctgaa gcactacggc aagaagcgcg
    25861 gcatcgcgag cctgtgcatc ggcggcggcg aggccacggc ggtggcggtc gagatgctct
    25921 gacgcggcaa ccttgtgggt cgccattgat cgtgggtcgc cattcatgcc gacccacata
    25981 aatgccgatc cacattagcc gctacaatgc gccgcaacgg aaacaaccgc gggatagaac
    26041 gacgatgacg gcaggcggca actgggatca cgcggtggac gtgctggtgg tgggctcggg
    26101 caacggcggc atgaccgccg cgctctgcgc ccacgagatg ggcgcgggca aggtgctgct
    26161 gatcgagaag cacgacaagt tcggcggcac cagtgcgatc tccggcggtg gcgtctgggt
    26221 gccgggcaac cgctacgcgc gcgccgccgg cgccagcgat tcgcgcgagg acgcgctgcg
    26281 ctacctagag gccacggtgc cggcggaatc tgtgccgcgc gaaatgctgg agacctacgt
    26341 cgacaacgcc ccgcgcatgc tcgacttcct gcacgagcgc acgcgcgtgc gctacgtcac
    26401 gctgccgcag tacccggact actactcgat gctgccgggt gcgcgcaccg ggcaccgctc
    26461 gatggaaccc gagccgctga tgaagtccga gctcggcgac gaggccgcga acctcgtcga
    26521 cacgcaccac atgatgtgga tgttcaaccg cttcgcgatc acgcaggccg aggcgcacga
    26581 cttcaccgcg cagctgccgg gctggtggaa gcgcgccgcg atgctggtga tccgctcggt
    26641 gctcgacctg ccgtggctgc tgaagtgggg gcgctcgcgg cgtatctgca ccggctgcgc
    26701 gggtatcgcg cgcctgcgcc tctcgatgat ggaacgtaac ctcccgctgt ggctgcgcac
    26761 gcagctcgtc gagctgctca ccgatgccga gggccgcgtc acgggcgcgg tgatcgagcg
    26821 cgaaggcaag cgcctgcgcg tgcaggcacg caagggcgtg gtgctcgcgg ccggcggctt
    26881 cgagcacaac cagcagatgc gcgagcagta cctgcccagg cccaccgagg cgcgctggag
    26941 cggcggcatc cgcaccaaca cgggcgatgc gatccgcgag ggcatgcgcc tcggcgcggc
    27001 gacgcgcctg atgggcgatg cgtggtggtg caccacgatc acggtgcccg gcgagccatc
    27061 accccggctc agcatcatgg agaaatccta ccccggctcc tgcgtggtga atgccgccgg
    27121 caagcgcttc accaacgaat cgcaaaacta catggcctac cagaaggcgc tgttcgccac
    27181 gcacagcaac gaaaatccct gcgtgccgag ctaccacgtc ttcgacgcgc gctttcgccg
    27241 cagctacatc gtcggaccgc tgtacaacag ccgcatgcgc ccggactggg cgctgccgca
    27301 gcgctggttc gaggccggct tcgtgcacaa ggccgatacc attgccgaac tcgcgcgcaa
    27361 ggccggcatc gatccggccg gactggagga gacgatccgg cggatgaacg agttcgcccg
    27421 cagcggcaag gacctcgact tccagcgcgg cgacgccgac tacgaccgct actacggcga
    27481 tccgcgcgtc acgcccaacc cttgcctcgc gcccatcgac gaggcgccct tctacgcgat
    27541 gccgatcgac ccgggcgatt tcggcacgca gggcggcctg gtcacgaata ccagcgcaca
    27601 ggtgctgcgc gaggacggct ccgcgatcga cggcctctac gcggtaggca actgcagcgc
    27661 cccggtgctg ccgacctacc ccggccccgg ctcaaccctg ggccccgcga tgaccttcgc
    27721 ctaccaggcc gccaagcaca tcacgggcta ccgcgcgtag gtcggcattc atgccgactg
    27781 gtgcgtgccc cgatgccgat gtcgccatga atggcaaccc acgttgatgg caaacctgct
    27841 gcatcagctc acgctctccg cgccgctgtt cctgctggtg ctggcgggct acggcctgat
    27901 ccgcgtcggg cgctgggacg atgcgatcgg cgacgcgctg gggcgcttcg tgttcgcggc
    27961 cgccctgccg gccatgctgt tccgcctgat gagcggcctg tcgacgctgc cgccggtgga
    28021 cgcgcgcctg ctcgtcgcct tcttcggcag ttgcctcgtg gtgttcgcgc tcgggcgtct
    28081 cgtcgcgtgg aagctgttcg cgctcgacgg cgtatcgcag tcggtgttcg ccctcggcgg
    28141 ggtgttctcg aacaacgtgc tgctcggcct gccgatcgcg cggctgctgc tcggcgaagc
    28201 ggcgctgccc gcggtcgcgc tggtgctggt gttcaactcg ctgatcctgt ggacgctggt
    28261 gaccgtcagc gtggaatggg cgcgccacgg cgagctctcc gcgcgcggct tcggcgccac
    28321 gctgaagagc gtgctgacga acccggtggt ggtcgcgatc ctctccggca ccgtgttcgg
    28381 catgagcggc gtcgcgctgc ccgccgtcct cgacaccacg ctcggcatgg tcgccgggat
    28441 ggccgcgccg ctcgcgctga tcgcgctcgg catgggcatc tcgcgctacc gcgtgcgcga
    28501 aggtttgcca ctggcaagcg cgatcatcgt gatcaagctg ctggtgcagc cgctggtggt
    28561 gtgggcgatc gcgcgcgcga tcgggctgcc gccgctggaa acgcaggtcg tggtgctgct
    28621 cgcctcgatc gcggtcggcg tgaacgtcta cctgatggcc tcgcagttcc aggtgctgca
    28681 gggcgcggtg gcggcgagca tcgtgctgtc cacgctcgcc tcgtcgatca ccacgccgct
    28741 gctgctggcg ctgttgtcgc catgaatggc gacctacggg ggtgcggtag cgccggcccc
    28801 cgtgggtccg gattcatccg gacacatgcc gccgcaggtc cggattcatc ctgagctacg
    28861 gggctaacgc gtgatcgtga attcgtcgag cacctgtccg tgctcgtcga cgaagcgcga
    28921 acgcagcgtg ctcttgccgg catcgagcac caccgagccc ttcagcgcga gcccgtgcgg
    28981 gcggtgcccc ggctcctcgc cgtcgaacgt gcggtgcgcc gggtgcgcca gccaggtctc
    29041 gtcgctgcac atcatgcgct ggcccggccg gcagggcttg gcggtatccg ccttgcccgc
    29101 gctgccggcc accgtgtaga cgacgcggtc gtcggcgccg ctcgcggggc tcagctggcg
    29161 gtagggcgct tcgccctgcc ccagcgcggg cgcgccggcc gcgtcgagcg ccgcgtgttg
    29221 tgcggcatcg aaggtatcgg ccatgccgaa atgcccgtgc aggtaccacg aacgctcgta
    29281 ggagtgcgcg tggccgctgt agaccgcgtc cacgccgtgc gcctggaaca ccggcccgaa
    29341 ggtctcgcgc atgtcgatct cggcctgctc cacgtccgag tcgtggttca tgcccttcga
    29401 gtagggcggg tggtggaaca ccaccacggt ccagtccagc cggttcgcgg aaaggtcgtc
    29461 gaccagccat gcgcgcatcg cggcgcgctg cgccgggtcg cggttcgaga tctgcgaatc
    29521 caggctgacc acgtgcacgt tgccgtggtc gaaggagtag tactgctcgg tgccgctggg
    29581 cacgccgccc gtctcgccgg ccgcgggcag cgcgaagatg ttcaggtacg gaaagccctc
    29641 cgcatccggc ccgtcgccgt cgctgtcgta gctgcgcgga tcggccgact cgctgctgcc
    29701 gccgagcggc atcggcaccg gccccttgtc gcagccgggc acgggaaaca gcgtgcatac
    29761 atccacgttc gtgacgccca tctcgtggtt gccgatcgtg ggccaggtcg ccacgcggtg
    29821 cagcaggctg ccgtagacat cgaagaaggc gccctgccac tgcgcgtcgg tgccggcgag
    29881 ataggcgttg tccccgagca gcagcaacag gtcgagcggt tcgtcgccgg cctgttcgcg
    29941 gctgtacttc aggtatccct ccatcaccgc gcgcgcctcg ccgcgatggc tggcccgccc
    30001 catgaactcc tcggtctcgg ttcccgaatc gccgaggatc cagatatgcg tgttgccgtc
    30061 ggcgggcacc tggcccgcgc gcggcgcggt gcggaaccac tgtcccgcgg gcgcgctggc
    30121 ggcgccgccg agcgagtagt agtacacggt atcgggttgc agcccgatca gcaccgcctg
    30181 gcggtgaccg gcgtcctcgc tggcgctcac acggatatcg agccgctcca tttccgtgcc
    30241 ggcgcagatc acgtcggcct cgccgtgcca gcgcacgatc gcgccggtgg ccgagacctg
    30301 ctgcaggaac agtcgactgg cgggcgtctc ggtgccggtg cacaccggcg ccggcggcgt
    30361 gcgctgcgcg gcgcaggcgc caagcagcgg gagcgcgatg attgacaggg cgcggatcca
    30421 cccgcggatt cccgggcaca cattccggcg gtctttgctc acgagctttc tcctcatagg
    30481 ctcagcgcac gttctcgatg cagcaggctg cgatcatcgg gctcagggtg cggctaacgc
    30541 ctgccctgag ctgccggggc acgcggcatg aacaccgccg gcgacacggc gaagcgccgc
    30601 gccagcgcat cgatctgccg cacgttgagc gcgcgacgtc ccgacaagac ttcggacacc
    30661 acgccctggc tgccgatttc gggcaacccg gattgcgtca ggccgtgctg ctgcatcagg
    30721 aagcgaagca cccccacggg atcactgccc tccggaagcc tgtgtgcgtc ctcgtagtcg
    30781 ccgatcaatt cccccagcgt ggccaccaga tccgccagcg ggtggtactc atcgcccgcg
    30841 ccggcatcca gcagttcgtt cagtgcacgt accgcctgcc tgtagtgggt cctcgtgcga
    30901 atcgggcgca gcggcacctg ctcgcaaagt gcttcgtaat ggcgtgtgag ttccttgaca
    30961 gcaagcatgc tcacttcctc cacttgtcgt actgcgcatg cgtgaacaca tgccggatga
    31021 acagcagctg acggtcaaaa tgaattgcag cgatcagccg atacctgtta ccagcaatgt
    31081 cgaatacgta gaaatcaccc acccggtcga ccgaattgaa actacgccgc agatctgcaa
    31141 agttacgcaa atgcgacgct tcgacgatgt gtcgccacgt tcgcagcggg tccgcggacg
    31201 caggatgcag cgtcgcgaag tcggtgagcg ccttgttgct gataacgcgc atgccggtat
    31261 ctccgatcag cggcacggta tctcacgttg agattgtttg taaaggactt gcaatccctg
    31321 cgtccacgtc gcgactcagc gcccgggcct gtttcaccgc agccgccagg ccgcgaccgc
    31381 ggcaggccgg cgaaaaactc ccgggctctc agtcccgcgc cccgagcccg cgcatcagca
    31441 actcgagaat gctcgccccc tcgtccgcgc cgacctcgaa cagcgggtag tcggccaccg
    31501 cctggcgcgt cagcaggctg tggatgttct gcaggctgtg cgcggtgcgg gccacgtcca
    31561 tgtcggcgcg gaactcgccg gcggcaatgc ctcgcgcgag cgcctcgcgc acccacgcct
    31621 gcgcatcgcg tatcgattgc gccgcggccg cgcggtcgag cagcaggctc ggctggaccg
    31681 cgtcggcgta gatgatcttc agcagcgtgc ggtggcgctc gccgggacgg atgccggtca
    31741 gcaaccagtc gcggatgccc tcgcgcgccg tgggtgccgt gcccagggtg gcgcgcatct
    31801 gcgcgagcag tgcgtcgatc aactggtggt tgagcgcgag cagcaattgc tccttgccgg
    31861 gaaagtgctc gtacagcgtc gagcgcgaca cgcccgcgag cttcgccacc cgggcgagcc
    31921 gcgcgtcgcg aaagccctgc gccacgaaga cctcggccgc cgccgcgagc agccggtcgc
    31981 gcacaccgcc cgcggactga cttgcgctat ccatgacgcc ctccttgcct ttggctttac
    32041 ccggacatta ttgaccatta atgtccgatg cgttagcgtg ccgctcacct ggactacgcc
    32101 aacggcaggc ccatgaaaat atcgatcacg atcacctcgg agtacaaccg cctgttcggc
    32161 cacgacttca cgcggctgat ggacgtgggg cgctgggccg aggcggccgg catcgaccag
    32221 ctcgacatcg ccgagcacct gctgatgggc gacggcaagt cctacccctt cggcgagtac
    32281 ccggcgccgc tcgacgaccc gtggccggag ccgatcgcag ccctcgccgc gatcgcggcc
    32341 gtgacgcaaa ccgtgcgcct cggcaccggc gtgctgatcg cgccgctgcg cccgccggcc
    32401 ctgctcgcca agcagctcgc cacgctcgac cgcatttcca acggccggct cgacatcggg
    32461 ctggccgcgg gctggcagcg cgaggagtac caggcctgcg cgatcccctt cgcgcagcgc
    32521 aaccagcgca tggacgacac cgtgcgcgcc tgccgcgcgc tgtggacggg cgagcgcgtc
    32581 acgctgaagc tgcccaccgt cgagctcgac aacgtgctcg cggtacccgc gccggtgcag
    32641 cagccgctgc cgatctggta cggcggtgca ccgacgccgg ccacggcgca gcgcatcgcc
    32701 gagttcggcc agggctgggt accgctgttc ctgcccgagg acgcgctcgc cacgggcatc
    32761 gcgctgatcg gcgaggcctt cgccgcgcgc ggccgcgatc ccgccacgct gcaggtgcgc
    32821 caccagctgt tcgcgcagtt cgatgccaat ggtcggctgg acctggacgc caccttcgct
    32881 cccgtggaac ggctcgcccg catcggcgtg acgatggtga gcctggggct cggctggagc
    32941 atccgcgacc cggccgacgt cgaggccacg atcgcggcca tcggcgcctg gggcaggaaa
    33001 cttcgctgac ccggcgagtc gcatcgcgaa cagaacctgg acacgggaga cactacggat
    33061 gagcaacacc ctggacggaa aatcggcact ggtgaccggc ggcgccggcg gcttcggcaa
    33121 ggcctgcgcg ctcgcgctcg cgcgcgacgg agcggccgtc acgctgatgg gacgcaacgc
    33181 cgcctcgctc gcgcaggcgc gcgcctggct gctggcgcag gcgccggatg cccgcatcgc
    33241 cgtgcacgcg ggcgacgcga ccgcggatgc cgacgtgcag gccgcgctcg cgagcgcggt
    33301 cgcgcacggc ggcggactcg acatcgtggt cgccacggtg ggcggcggcg gctaccgcac
    33361 ggtgctggag caggaactcg cgaccttcat ggccgacatg cagctcaacg tcggctctgc
    33421 cttcgccgcg gtgcaccacg gcgcgccgct gatgaaggac ggcggcgcct tcgtgttcat
    33481 ttcctcgacc gccgccgtgc tgtcgttcca ggggctgtcg agctactgcg ccggcaaggc
    33541 ggcgctcgac cacttcgtgc gcgtcgcggc cgacgagctc ggctcgcgcg gcatccgcct
    33601 gaactgcgtg cgccccgggc tcacgcacac cgacggcatg gacccgttct tcgcgaaccc
    33661 ggcctacgtg gaatcgttca agccgctgat cccgctggga cgcaccggca tgcccgtgga
    33721 cgtcgcggcc gcggtccgct tcctggccgg ccccgagtcc tcgtggatca ccgggcagag
    33781 cttcgcggca gacggcggca acgagctgcg cggcgcgccg cgcggctgag cgccaccgta
    33841 tctcgaaggc ccgccgcttc agctgaacgc gtgcgcaagg cccggccgcg cgtcatcgac
    33901 cagcacctcg cccggctgca gccgcggctt gcgcggccag tttccgggcg ccgcgcggcc
    33961 caccgcgatc atcatcaccg gcaggtaacg ctcgtcgatg tggaactcgc ggcgcagcgc
    34021 ctccgggtcg aagccgatca tcggcccgct ggcgaggccg cgatccgtgg ccgcgagcat
    34081 cagcgccatc gcggccatgc tggccgagcg gatagcctcc tcgcgggcaa atgccggctt
    34141 gccttcgtag gcaccctgcc cgctcgccgc gaaaccggct gcgccgggcg catcgagcag
    34201 cccggcctcg cgcgagcgct cggcgatcag cggcaggcgc tcggcgccac gcgtatcgcc
    34261 cagcaccacg aaggtggccg cggcatcgcg cactttctgc tggccgtagg ccacctgctg
    34321 cagcgccgcg cgtgcagcag ggtcgcgcac caccaggaag cgcgcgtgct ggatgttgta
    34381 ggccgagggc gcctccatcg cggcggcgac cagtgcctcc acctcctcgg gtgccagctc
    34441 gtgggtggtg tcgaacttgt tggcggaaat gcgcgccgcc agcgcctgtt gtacgttcat
    34501 gcctgtctct ccttgttgaa tggtcatcat tgcgccagcg ttcccgcgcg gtaatccgcg
    34561 atggcctggc gaatctcgtc ctcggtgttc atcacgaacg gcccgtagtg cgcgatcggt
    34621 tcgccgagcg gccggccggc cagcaccagc aggcgcgcgc cggcctggcc tgcgtgcagc
    34681 gcgagcgcat caccggcgcc gaagatagcc gtctgtccgg cctgcaccgg ctgttcgccg
    34741 gcgagcgcgc cgtcgatcac gtagaccagc accgactccg cggggggcac cggcagcgtc
    34801 gtggcgctgc ccgcgggcag tacgagctcc agcacccgcg cgccggcggc aaggcggtcc
    34861 agcgggctcg cgacttcttc gccctcgagc tgccagcgcc cgccgaagac cttggcgcgc
    34921 gcgccgcccg gcaactcgcg ctgcggcagc gcggcggcat cggcctgcgc gtactcgggc
    34981 gctttcatct tctgggccgc cggcaggttc acccacagct ggaagccgtg caggcggcct
    35041 tcctgcgcca gcggcatctc ggaatggatc acgccacggc cggtggacat ccactgcgcg
    35101 ccgccgtcgc gcagcgcttc gcgatgcccg aggtggtcgc ggtgctcgaa gccgccgtcg
    35161 aacatcagcg tgaaggtctc gatgcgcggt gcgggtgggg cgggaagccg cccatgtagt
    35221 ccgccgcctc gtcggcgcgg atctcgtcga tcatcaggaa gggatcgagg tcgtggcggt
    35281 gcaggcccag cgtgcggcgg atcttcacgc cgtcgccgtc cgaggtgggg cggctgtcga
    35341 ggatcttgtc gatgcggcgg gtgttcatgg cgctcactcc gtgcggtttg aaggattgac
    35401 accagaatac atccgttgga taaattgaaa accgcgattc ttcgctataa acgatcgaga
    35461 atatcgatga ctactgcaac gctggaacag tggcgcctgc tggccgccgt ggtggaccat
    35521 ggcggctttg cccaggcggc cgaacagctg cacaagagcc agtccaccgt gagccacggc
    35581 gtgaagcagt tgcagcaggc gctgggcctg ccgctgctga cgctggccgg gcgcaaggcc
    35641 gtgctcacgc ccgggggcga gaccctgctg cgccgcgcgc gctacctgct ggaggaagcc
    35701 gcggcactgc agcatctcgc cgacacgctg gcgcagggct gggccgcgcg cgtgagcctg
    35761 tcggtggatg ccatttttcc gcaggaggcc ctgttcgagg cgctggccgc cttcggccgc
    35821 gagagtcccg acacgcgcgt ggaagtgcag gaatcggtgc tctcgggcgg caccgaggcg
    35881 ctcttgcaaa ggcaggccga actggtgatc accggtcggg tgccgccggg ctttctcggc
    35941 gagccgctgc tgcgtctcgg gttcatcgcg gtggcccacc cggcgcaccc gctgcatcaa
    36001 atcgggcgcg ctctgcgcct ggaggacctg cgggcgcacc agcaggtggt gatccgcgat
    36061 tccggggtgc ggctgcgcgc ggattccggt tggctgggtg ccgagcggcg cctcaccgtc
    36121 tcgcatccgg ccacctccat ccaggcgctg tgccgcgggc tggggtttgc gtgggtgccg
    36181 cgcgagaaga tccgcgcgga actcgacagc ggccagctga aagcgctgcc gctggtcgag
    36241 ggcgccacgc gctgggccga gctctacctg gtgctggccg agcgcgaggc caccgggccc
    36301 gccgcgcgac ggcttgcaga gctgctgcgc gaggccgccg cgcgctgccg cgcgcacgcc
    36361 gacggcggcg ccaactgaca tgccctgctg tgcggaggga acggctcgcg ctatcgtgcg
    36421 caacggcacg cagcagggtg gcatcggatc atgatggacg cggttctcct ggccggcggc
    36481 ttcgtcgtgc tggtgctggg cgcagagctg ctgatacgcg gatcctcgcg cctggcggcc
    36541 gcggcaggca tgccctcgct ggtcatcggt ctcaccgtgg tcgcctttgg caccagcgcg
    36601 ccggaactgg ccgtcagcgt cggctcgacg ctcggcggct cgcccgacat cgcgctcggc
    36661 aacgtggtcg gcagcaacat cttcaacgtg ctgctgatcc tcgggctctc ggcgctgatc
    36721 gcaccgctga cggtgtcggc gcagctggtg cgtttcgatg tgccggtgct gatcggagtc
    36781 tcgctgctgg tttggctgat ggcgctggac cacgcgatcg gtcgcctcga gggcgcggtg
    36841 ctgggactga tgctcgccat ttacctcgat gtccagatcc gcatgggccg gcgcagcgac
    36901 ggccgcgacg tgcccgtcgt accggcggcg cgcggccgcg ccgcgttcgc ggccaatgtg
    36961 ctgctgatcg tcgcgggact ggtactgctg gtggccggcg cgcgctggct gctcgccggc
    37021 gccaccggca tcgcgcgtgc cgccggcgtg tcggagctcg tgatcggcct gacgatagtg
    37081 gcggccggca cctcgttgcc cgagttggcc acctcggtgc tggcggcgct gcgcggtgag
    37141 cgcgacatcg cggtgggcaa cgtgatcggc agctgcatgt tcaacctgct cgcagtgctg
    37201 gggctgaccg ccgcgctggc gcccaccggc atccgcgtgg ccggggccgc gctggccttc
    37261 gacatcccgg tgatggcagc ggtcacgctg gcctgcctgc cggtattctt ccgccgccag
    37321 ctcatcgcgc gctgggaagg cggcgtgttc acgctttatt acgtcgccta tgcgctgtac
    37381 ctcgggctgg cggcgagcgg ccaccaacgc ctcgacggct accgcgaagc gatgctcggc
    37441 ttcgtgctgc cgctgacagt gctcaccttt gcgctcatcg cctggcgcag ctgggtcaag
    37501 gacggcgcgc catcgcgctg agcgggcatt cctgccgccg cgcgcccttc cctgccgcgg
    37561 tgcggcaggc tatggtgcgc ggcagtttgc agccgggata aaggtccatg tcgaacgtgc
    37621 ttctcctgat cggcggattc gtggtgctgg tgctgggcgc cgagatactg gtgcgcggcg
    37681 cctcgcgcct ggccgcggcc gcgggcatgc ccccgctgat catcggcctc accgtcgtcg
    37741 ccttcggcac cagcgccccg gagatggcgg tcagcgtcgg ctcggcgctc ggcggctcgc
    37801 ccgacatcgc gctgggcaac gtggtgggca gcaatatctt caacgtgctg ctcatcctcg
    37861 ggctctcggc gctggtcgcg ccactggcgg tgtctgcgca actggtgcgt ttcgatgtgc
    37921 cggtgatgat cgcagcctcg ctgctggtgt ggctgatggc gctcgaccag tcgatcagcc
    37981 gactcgaggg gctggtgctg gcactgatga tcgcgatcta catcgacatg cagatccgca
    38041 tggggcgccg gggcgacgat ggcgatgtgc ccgcggtgcc ggaggcgcgt ggcgccggtg
    38101 cgatggcgct caatgcggtg ctgatcgtcg cgggcctcgt gctgctggtg gccggctcgc
    38161 gctggctgct cgcgggtgcc accggcatcg cacgcggctt cggtgtctcg gagttgctga
    38221 tcggcctcac gatcgtggcg gccggcacct cgttgcccga gttggccacc tcggtgctgg
    38281 cggcgctgcg cggcgagcgc gacatcgccg tcggcaacat cgtgggcagc aatatcttca
    38341 atattttcgc ggtgctgggc ttcagcgccg cgctatcgcc cgccggcatc cacgtggccg
    38401 aggcggcact ggtgttcgac atcccggtga tgaccgccgt ggcgctcgcc tgcctgccgg
    38461 tgttcttccg ccgccacctg atcgcgcgct gggaaggcgg cgtgttcgcg ctttactaca
    38521 tcgcctacac gctatacctg gtgctcgcgg cgaccgcgca ccagcggctc gacgattacc
    38581 gcgaagccat gctcggcttc gtgctgccgc tcacgctgct caccctggtg atcatcctgt
    38641 ggcgcacctg ggtgggacgg aaggcctagg tcggcattcc gtggggcggc atttacgccg
    38701 acttgtgcct gcccggatgc tgctgtcgcc atgaatggcg acctacgggc atgaatggcg
    38761 acacaccgtc agcggcggaa ctggcggaaa tcgggggggc gcttcgcgat gaaggcctcg
    38821 atcgcctcgc ggttctcggg agtgccgacg ctgcgcacca tgcccgcgtc ctcggccgcc
    38881 agcgcggcgt gcaggccggc gcggtgcacc gccttcagcg tgcgcttggt ttcctgcaat
    38941 gcgctggtgg gccactgcgc gatctcgcgc gctttcgcca gcgcatgcgc gaacagctcc
    39001 gcgtcaggca gcaccgcggt ggcgatgccg gtctccagcg cgcgcgtcgc cgtgatccat
    39061 tccgcggtga acagcagctc cgccgcgcgg cgcgcgccga tgttcgcctg caactggtag
    39121 ctggaagcaa attccggcac cagccccagg ctcacgaagg gcaggcgcat gcgcaggctc
    39181 tcgcccacgt acagcacgtc gcagtggaac agcagcgtcg cgccgccgcc gaccgcgatg
    39241 ccgttggccg cggcgaccag cggcttgtcg aaatcgcaca gcagccgcgc acagcgcgcg
    39301 aagggatggt cggccccggc gtcgctgaac tcgtagaggt cggtgcccga ggagaagttt
    39361 ccgccggcgc cgctcaccag caccaccgcg acgcgcttgt cgccctgcgc ctcggcgatg
    39421 gcgtcggcga aagccgccca ctgctcgaaa ttgaacgcgt tcttgcgctc gggccggttc
    39481 agcgtgagct tcagcacgcc gtcgtcgagt tccttcagta tcgtgtcgct catgtgtgct
    39541 cccgcgcggg tggccgttcc agggctcagc tcacgaagcg cacgttgtcg gccagcgccg
    39601 cgcggccgag ccggcaatcg ttcgccgcat tcgcggtgtc ctcgtagccg aaggagatgc
    39661 cgaacagcag ccggtgcgag gcgtccacgc cgcactcggc gcgcacctgg tcgcagagga
    39721 agctgagcgc cgtctgcggg cagctcccga gcccgtgggc cgcgagcgcc agcatcagcg
    39781 tctgcgcgta catgcccgcg tcggccgcct cgcgcacacc ggcccagtcc gggatgaaca
    39841 ggaaggcaac gtgcggcgcg tcgaagaaag cgaagttgcg catgaacgcc gcgccccgcg
    39901 cggccttgtc ctcgcgcgcg atgcccatcg cgccgtagag cttttcggcg gcgtcgtact
    39961 ggcgctcctt gtagacaccc gcgtacttgc cggcataagg gaagtccatc gagacctgcc
    40021 ccgaggcgaa atccgccatg aagcgctggc gcaggcgctc acaggccgct ccgctcgcca
    40081 ccgcggcgag ccagggctgg gtgttgcagt tcgagggtgc gcggttggcc agcgcgaaca
    40141 catgctcgat cagcggctgc ggtacgggat cgggacggaa ggcgcgcacc gagcggcgcg
    40201 ccagcacggc ggcatcgaag ctcagggaat cgtctttcgc cgtgactgct gctgcaatgg
    40261 tcgtcatgga tgaagggtcc ttggctgacg ggatggcgtt cacttgccct tgaactcggg
    40321 cgggcgcttt tccttgaagg ccttcatgcc ctcgcgcgcg tcctcggtgg cgaataccgg
    40381 ccagcccacc tcgtcggagc gcagcatcgc gtcggcttcc gccaggcact cctcgtgctc
    40441 gcgcagcgac ttcatcaccg ccttcacggc cacgggtccg ttcctgccga tctgggcggc
    40501 gatcgccagt gcctcgtcca gcgtcttgcc cttgggcacg atgcggttga tgaggcccca
    40561 ctcgagcgct tcctgcgccg tcacgtggcg cccggtcagc aggatctcgg cggcgaggca
    40621 gtagggaatc tggcggcgca ggcggatcgt ggagccggac atcgggtaca gcccgcgcgc
    40681 gacctcggtc acgccgaaca ccgcgtcctc ggccgccacg cggatgtcgg tgccctgcag
    40741 gatctcggtg ccgccggcca gcgcgtagcc ttccaccgcg agcacgatcg gcttcagcgg
    40801 gcggttgtcg cgcagcagcg cctgccagtg caggttcggc acgtccttca tcagcgcctg
    40861 gatctcgtcg gcctccgcgc cgccgcccga ggggccggtc ttcaggtcca tgccggcgca
    40921 gaaggtgtcg ccacgcgcgg tgagcacggc gcagcgcagc gagtcgtcgc tgtcgagcag
    40981 gcgccaggcg cggtacatcc cgaccagcat cggcgggttg aaggcgttct tcgcctccgg
    41041 gcggttcagg gtcacgacca gcgtgtgtcc gtccctctcc acgaggcagg tgccggtgcc
    41101 gatgctcagt tgttccatgg ctgtgtttcc tgtcgtgata atcggggacg attggcgttg
    41161 cccgcggccg tgaccgtcgc ggccatgggc cgctcctacg gcgcgcggtg cgtgccggcc
    41221 agcgcgattt ccttcgcgcg cgcgtaattg ggcttgccgt tcggctggcg cgggatctcg
    41281 ccgaccacgt gcagctcgcg cggcaccttg tagccggcga tgtgcttgcg cgcctcctgc
    41341 tgcagctcct ccagcgtggg cgccgcgccg cgcggcgaga tcaccgcggt gaccttctgc
    41401 ccccagcgct cgtcgggcgt ggcgacgacc agcgcatcga acacggccgg gtggttcttc
    41461 agcgcctgct cgacttcctc ggggaagatc ttctcgccgc cgctgttgat gcagttcgag
    41521 ccgcggccga acaccgtgat cgagccgtcc atttccagcc gcgccgagtc gccggtcagc
    41581 agccagcgct tgtcctcgac cgtcacgaag gtcttggcgg tcttcaccgg atcgttgaaa
    41641 tagccgaccg ggatgtggcc ggagcgcgcg aatatgccca tctcgccagg ctgcgcgtgg
    41701 cgcagttcct cctcgacgat cacgtccatg tacggcgtgc gcgtcacgtt gccgaggccg
    41761 ttctccgcgt ccgcgcggcg ctggccgttg tccatgccca tctggccgcc ctcggaggaa
    41821 ccgaaggagt tcgtgatcaa cacgttcggg aaatgctcgc ggaacttcgc ctgcttggac
    41881 tcggagaaca ccgcgccgcc cgagcccacg ttgaacacgc aggagagatc ccagcgcccg
    41941 gggttctgcg ccagcacatc gagcagcggg atcgccatcg cgtcgccgac gatcgagacg
    42001 gcattgatgc gctcgcgcgc gacgatgtcc cacacctcct cgccgtcgag atggtggctc
    42061 gggttcagca ccagcttgcc gcccgccagc agctggatgc aggcgtacca ccagcaggcg
    42121 ccgtgcatca gcggcgccag cgccatgccg gtgaccacga agccatcctt cgcgcgcacc
    42181 gcgatgtcct cgggctgctc gatcggcccg gccgggtgga agtggccgcc gccgcccatc
    42241 gcggcgtaga acaccgcctt gtgcggccac atcacgccct tgggcatgcc ggtggtgccg
    42301 ccggtgtaga gcaggaacag gtcgtcgtcg gagcgctcgg cgaaatcgcg cgcgggcgaa
    42361 ccctgcgcca gcgccgcgtc gtagccgatg gcgccgatcg tggccggatc taaggccgtg
    42421 ccgtcgtcca ccgcgatcag cgtcttcagc ttcggcgcgg cggcgcgcac cgccgcgagg
    42481 tgcggcacga actcgcggtg gtggatgcag ccggccatct cggcgttgtt caggatgtag
    42541 gacagttcct cgtcgacgta gcggaaattc acgttgaccg gcaccgcgcg caccttgaag
    42601 caggcgagca tgccctcgag gtattcgttg cagttgtaca ggtacagccc gaccttgtcg
    42661 ccggcgccga tcccctgcgc ggccaggtaa tgcgcgaggc ggttggcacg ctcctcgagc
    42721 tgcgcgtagg tcaggcgcgc atcgccgcag accagcgcct cgcgctcggg caccgtgtcg
    42781 gcgacaattt cgaacaggtc ggcgagattg aaatgcctgg ctggcatgaa tgcgtgctcc
    42841 ggtggccgtg ggaggacggc agaggatacg cgagggcccg gaggcgcgac aaatgacaaa
    42901 ccggcaatca agcacgcggg cgcggcgcgc agcggctcag ggaaccagcg ccgcctgcat
    42961 gttgtgcgcg atgtgtccgg ccacccggcg cgcgcgctcg gcgtgcggcc cgctgtagcc
    43021 ctcgtcgagg gtggtgcaga acagcgccag ccagcgctcg aagtcggccg gctcgagctt
    43081 ctgcttcgcg tgcagcgcgc ggtggatgtt catggtgtgg cgccggtagc tgtcgctgcc
    43141 caggatgagt ttctcccagt acgagcagat gatcggcagg tgcttcgcga ggtctatcgc
    43201 agcgacgtcg aggaagatcg gggcgagccg ctcgtcggcg agcacgcgcg cgtagaaggc
    43261 ctcgatgaag gcgcggatgc gcgcggggga gtcgaggtcc ggcagggggc tagcgcttgc
    43321 tcttcctgac atgctgcatc atccgcttgc gcttgtattc ctggcgcggc gtcagcgtgt
    43381 tgcgcttgcc cgcatacggg ttcgttccgg tcacgaactc gatgcgcagc ggcgtgccca
    43441 ccagcttcag ttcgcgacgg tacacgttct ccaggtagcg gcggtagtgc tcgggaacgt
    43501 ccgcagtctg gttcccgtgg accacgatca ccggcgggtt gctgccgccc gggtgcgcca
    43561 tgcgcagctt gatgcgccgc ccgtgcacca gcggcggcgg gtgcgacgcc acggcatcct
    43621 cgaggatcgc cgtgaggcgg ttggtgggca gcttgcgccg cgccgatgcg taggcacgat
    43681 cgaccgcggc ataaagctgg cccacgttcg agccgtgcag cgcggagatg aaatgcacct
    43741 cggcgaaatc ggcgaacatc agccgccgat cgagctcgct cttcagggcg gccttcctgt
    43801 cggtgccgag accatcccac ttgttgcaga cgatgatcaa cgagcgcccg ctctcgatca
    43861 cgtgcccgat caggtgcacg tcctgctcga acagaccctc gctggcatcg accagcacga
    43921 gcgccacgtt ggcatcggcg atcgcctgca gcgtcttcac gatggagaac ttctccaccg
    43981 tctcgtgcac gctgcgcttg cgcctcaggc ccgcggtgtc gatcagcgtg tagcgcttct
    44041 cgccgcgctc gaaatcgacg tagacgctgt cgcgcgtggt gcccggctgg tcgaagacca
    44101 cgacgcgctc ctcgccgagg atgcggttca ccagcgtgga cttgcccacg ttggggcgcc
    44161 cgaccaccgc caccctgacg cgctcctcgc ccgcctcgcc ttcctgcgcc tgcggctcgc
    44221 cggcatccgg gaaggcgtcg atcagtgcct gctgcaactg ctgcacgcca cgcccgtgcg
    44281 aggcgctcac catcaacatg gtggccgcgc cgagctcgtg gaactccgcc ccggagagca
    44341 gctcatcggt gccgtcgatc ttgttgacca ccagcacgaa cggcttgccg ctctcgcgca
    44401 gcagcgcaac gatgcgctgg tcggccggcg tcacgcccgc gcgcgcatcg accagcacca
    44461 gcgcgaggtc gcactcggcg accgcggcca gcgactggcg cgccatctcg gcatcgatgc
    44521 cggcctcctc gccgctgagc cccccggtgt cgatcaggat gaagcggtgc tcaccggcgc
    44581 ggaactcgcc gtactggcga tcgcgcgtga gccccgggat gttggcaacc agcgcatcgc
    44641 ggctgcgcgt gagacggttg aacagcgtcg acttgccgac gttgggccgt cctaccagcg
    44701 cgatgacggg cagcatcgtg gcgcctctca gcggctctcg acacggtagg cccgcagccc
    44761 gccgtcgttg ccgtacacgt acagcatgtc attgcccgtg accatgtcgg cgcgcacgcc
    44821 gtcgccatcg gcgcgcacgc gtccgacgaa atggccgtcg gcctgcgaca gcaggtgcac
    44881 gtagccgtcg aaatcggcca ccgcgacatg gctgccgatc gccgccggcg tgccgggctg
    44941 gcgccgtgcc agctggtcgt tctgccagcg cagcgcgccg cccgcggcct cgtaggcgct
    45001 caccgtgccg ctcgcctccg ccacgtagat attgccgaag ccctcggcga tgccgacgta
    45061 gctcgaggca tcgcgcgccc acaccgggcg cccgttggcg atgttcaggc cgccgagctt
    45121 gccctggtag gtcacggcga acacattggc gccgaccagc agcggccggc cctcgatgtc
    45181 gatggtgcgc tcgatgtccg attgcccctg cggattggcg atgcggccat cccacagcac
    45241 cgtgccggtg tcggccttga tggccacgat gcgtccgctg gcgagcgccg catacgcgac
    45301 accctcgctc agcagcggtg cggcggtgcc gcgcagcgtc agcagcggat tgctgctgtc
    45361 gtgcgtccag cgccgcgcgc cggtcgccgc gtccaggccc gtcaccttgc cgtcgagcgt
    45421 ctgcaccacg accacgtccc agtccgccgc gggtggagcc agcacctcgc tgctcacgct
    45481 cgcgcgccac agctcgcgcc ccgtcgcgct ctccatcgcg atcacgcgcc cgcgggtgtt
    45541 gcccaccagc accaggtcgc cgcccacgcc cacgccgccg gagagctcgg tatcgacgtc
    45601 gaccttccag cgccgcttgc cgctcgccgc atcgagggcc accaccgtgc cgttctgcgc
    45661 cgcggcatag atcgcgtcac cgtagagcgc gggctgcagg cggttgtaca gtttcccctg
    45721 gccgttgccg acgccggtgc tccagacctt gcggatgtcc acctcggggc tgaactccac
    45781 cagctcggcc ggcgcgagcg cggcatcctc ttcgtcgtcg ccgatgctct tgacgtaatc
    45841 caccatgccg caggcgccca gcatgcccgc gagggatgcc gcgagcagtg cccgcagcag
    45901 cgccgggccg agccgtttct tctcgccgat catcattgct tggtcgctcc cgtgtcgggg
    45961 gtcgtggcat ccgccgctgc cggatccgca gccgccgggg cagcgcccgc ggcgggctcg
    46021 cccagctcca gttcctgcag cttgagctgc agcaggcgcg acgccgtggc gccctcgcgg
    46081 ctgccggcac gctccaccgc cgcgcgatag gccgcgatgg cagccgcggt atcgttgcgc
    46141 cggctcagga tatcgccgcg cagctccagc ttctcgaccg cgaaatcgtc cgtgcccgcg
    46201 gcatcgagca cggccagggc atcgtcggag cgccccagcg cgagcagcac gcgcgccagc
    46261 cgcacgcgcg ccagcgcgcc gagctgcgga tcggcgtacc gcccgagcag gccgtcgatg
    46321 cgtgtggcca gcgcgccgcg cgccacgtcg ggctggcgct ccagcagggc gcgcagctcg
    46381 gcctcggccg cgatgagttc gttcttcccg acgtgcaggc gcgccagcat cagcgaggcg
    46441 tagtcggcgt agcgcgaacc gggactcagc tccttgagtt gcgccgcgag atgcgcggcg
    46501 gtggtgccct ggatcgggtc ggcggccgcc gcatcgacgg cctgcagcag ctgctggtag
    46561 gtgacggccg cctgctcgct cgcgccctgc tggcgctgct gccaccactg ccagccgaac
    46621 accagcgcca gcgccagccc gacgccgatc agggtcgagg agccgttgcg cgcccaccac
    46681 tccttgagtg ccttgatctg gtcttcttca gtaccgtaag gatccacgat ctctccccgt
    46741 tgcgctctct ctgtgcgcca ccgctcagcg cgtgacgatc tgttgtgcca ggtaatccgc
    46801 cagcgcgtcg gcgcccacgc ggcactgctc cggcccgccc tcgcgcagcc cgcgcacgct
    46861 gacttccccg gcggccagct cctcctcgcc cacgatcagc accagcgagg cgccgctgcg
    46921 gtcggccttc ttcatctgcg ccttcatgcg accttcgcca caatgcatct gcagccgaag
    46981 ccacggcagg cgctcacgca ggcgctcggc gagcacgaag gcgaagcgct gcgccgccgc
    47041 gtcgcccacc acccagtagg catgcggctg cgcgcggtcc gcgggctggc tcccggccac
    47101 ctcgagcagc aacaccaggc gctccaagcc catcgcgaaa cccacgcccg gcacgggcgt
    47161 gccgccgagt tgctccacga gcccgtcgta gcgcccgccc gcgcataccg tgccctgggc
    47221 acccaggctg tcggtgaccc attcgtacac ggtcttgcca tagtaatcga ggccgcgcac
    47281 cagcgccggg ttcacgcgat aggcgacccc cgcggcgtcg agcagctcgc gcaggccgcg
    47341 catgtgttcg cgcgattcct cgtcgagatg atcggtgagt tgcggcgcgc ccgccagcag
    47401 ctcgcgcgtg cccggatcct tggtatcgag gatgcgcagc gggttgctgc ccaggcgccg
    47461 gcggctgtcc tcgtcgagct gctcggcgcg cgcggcgagg tagccgacca gcgcctcgcg
    47521 gtaattcgcg cgggcgacgc tgctgcccag cgaattgatc tccagcgtca ccaggcgatc
    47581 gatgccgagg cggcgccaca ggcgcgccag cagcagcagg tgctcggcct cgatatcggg
    47641 ccccggcagc ccgaagctct ccacgccgat ctggtggaac tggcgctggc gccccatctg
    47701 cgggcgctcg tggcggaaca tcggacccag gtaccacagc cgctgcggca aggtgtgcag
    47761 caggccgttc tgcagcaccg cgcgcacgca gcccgccgtg ccctccgggc gcagcgtcag
    47821 gctgtcgccg ttgcggtcgg taaaggtgta catctccttc tcgacgatgt cggtgacctc
    47881 gccgatggag cgctggaaga gctccgtggg ctccagcagc ggaaagcgga tctcgcggta
    47941 cgcataggcg tgcagcagcg acgtcacctc gtgctcgatg tgctgccaga gcggggtctg
    48001 ggaaggcaga atgtcgggca ttccgcggat ggccctgagt tgctgcaacg cgctggtctc
    48061 cggtttcaac aggcgaaccg ccggcgccgg gggcaggcgg agcaaaaaaa catgcgcacg
    48121 gtatagccgg aatcaggctt tggcaatgag gctcccggcg gcggaatcgc gcgccgcggc
    48181 ctgctcggcc agcgcggcgc ggattgtctg ctcgagatcg tcgatcagcg agccggactc
    48241 caccttgtga tgtggcttgc cgccgacgaa caccaggttg cgcggcgcgc tgccggtgac
    48301 gcccacgtgg gcctcgcgcg attccccggg tccgttgacg tagcagccga tgaccgccac
    48361 gtcgagcggc tcgcgcacgt cctcgaggcg acgctcgagc tcgttcatcg tcgcgatcac
    48421 gtcgaagttc tgccgcgagc agctcgggca ggccacgaag tttatgccct tggcgcgcag
    48481 gcgcatgctt ttcaggatgt cccagccgac cttcacttcc tcgaccgggt cggccgcgag
    48541 cgagatgcgg atggtgtcgc cgatgccgtc catcagcagc atcccgagcc ccacggatga
    48601 cttcacggtg cccgcgcgca ggccgccggc ctcggtgatg ccaagatgca gcggctgctc
    48661 gatctgcggc gcgagcttgc ggtaggcctc gaccgtcatg tagatgtcgg aggccttcag
    48721 gctgaccttg aagtcgtgga aatcgtgctt gtcgaggatg tcgatgtggc gcagcgccga
    48781 ctccaccatc gcgtcggcgc agggctccgg gtacttgcgc tgcagttcct tctccagcga
    48841 gccggcgttc acgccgatac ggatcgggat gccgttgtcg cgcgccgccg cgatcaccgc
    48901 gctcacgcga tcctcgcgcc cgatgttgcc cgggttgatg cgcaggcagt ccacgccggt
    48961 cttcgccacg gcgagcgcga tcttgtagtc gaagtggatg tccgccacca gcggcacgcc
    49021 gacctgcggg cggatcttcg cgaaggcggc cgcggcatcc atgctcggca ccgagacgcg
    49081 cacgatgtcg gcgcccgcgg cggccagctg attgatctgc ccgaccgtgg ccgcgacatc
    49141 gcaggtctcg gtgttggtca tgctctgcac cgagatcggc gcatcgcccc cgaccgggac
    49201 cgaaccgacc atgatgcggc gcgagcgccg gcgcacgatg ggtgatggat gcagcatggg
    49261 cggcgctgcc tcagggtgcg gcgcgcggcg gcgcgagcag cggcaccacc tgcggcccgc
    49321 tggcggtgtc gacggtcacc gcggcggtgc ccggctcgcg cacggcggcc tgcggctcgc
    49381 gattctggaa caggaacagc gcgctcacgg cgagcagcgc cagcaccatg cccacgcgcc
    49441 cggcacccga ggcgccgcgc cagcggtcgc gcttggccac gctcgcacgc ttctcggtct
    49501 tgcccgcggg gctcgtcacg cgcaggcgcg acagcacctc gtcggcgtcg atgccgagca
    49561 gccgcgcgta gttgcgcagg tagccgcgca cgaaggtctc gccgcggatg cggtcgtagc
    49621 agtccttctc cagcgagttc accacggact gcagcaggtg cagcctgtcg gcgacctgct
    49681 ccacgctcat ccccgcggac tcgcgcgccg cgcgcagcat ggcgccgggg ctgcgctgat
    49741 ccgtgctcga cgtgtcttcc tgggccatgt tgtgtcagcg ctcgctggaa gtactggaga
    49801 actcccggta ttccgcggaa tccgggtaca tgcttttcag ctgcagggag tagctggcct
    49861 cggcatcgcg atcgcccgcc aggcgcgcga ggcggatgcc gagcagcagg ctgcgcggcg
    49921 tctggcgcgg cgccaggcgc cggaactgct cgtaatagcg cgccgcctgc tcatggtcgc
    49981 cgcggtcgta gttgatcgcg gcgagctcga gcatcgccgg ccactggcgc cggtccatct
    50041 tgaccgcgcg cgcgagcgct tcctcggcct tcgcggtctc gcccaccttc tggtaggcgc
    50101 gcccgaggtt cgcgaacgcc gcgccgcgcg attcgtacag cgtgtccgcc accacctgct
    50161 ctagctcgcg gatcgcgccg cgcgtgtcgc cacgctcgta gaggaaggcg gcgtagttgt
    50221 tgcgcgcgcg cgagaacttc gggtcggagg cgatcgcctt gcgaaagtgc tcctgggcca
    50281 gctcgtattc gccggtcttc cagaacacct gtgccagcgt gtcgtgcacc ggcgcggcgt
    50341 tcgggtcgag ctccagcgcg cgcttgaggt gcacgatcgc cttttccgtg ttgccctggt
    50401 tcaggtaatc ggtcgcggcc ttgacgcgac tggccacctc ggcctgcttg tcggccttga
    50461 acgtggaatt ggtggtggtc acgcagccct gcacgagcag cgccagcacg agcggcgcca
    50521 gcagccggat ccacgggccc gggccacgcg cgctcatgcg cgaacccccg gcgcgcgatc
    50581 gcaggcaagc gccgcgcgat ggcgcgcgct gcgccgcgtg cggtcctgca cctggccgac
    50641 cagctgcccg caggcggcgt cgatgtcgtc accgcgcgtg gtgcgcaccg tgacgcggtg
    50701 cccggcctcc gtgaggacgc tccagaagcg gctcacggca ttgcggctcg gtcgctcgta
    50761 ggacgattgc gggaaggggt tgaagggaat caggttgatc ttgcacggat agccgcgcag
    50821 cagggccgcg agctcgcgcg cctgttcgac gctgtcgttg acacccttga gcagcgtgta
    50881 ctcgaccgtg atcacgcgct tgctgtcggt ctgcgcgtcc atgtagccgc gcgcggcatc
    50941 gagcaggctc gcgatcgggt agcggcggtt gaccggcacc agctggtcgc gcaaggcgtc
    51001 gttgggggcg tgcagggaga tcgcgagcga ggcttcgctg acggcggcca ggcgctcgag
    51061 cgccggcacc acgcccgcgg tgctcagcgt cacgcggcgc ttggagatcc cgtaagcgaa
    51121 gtcgtccatc atgagatcca tcgccgccac gacattgtcg aagtttagca gaggctctcc
    51181 catgcccatc atcaccacgt tggtgatgct gcgctggcca ccgcgcttgg gcaggccaaa
    51241 ggaacgcgcc gcgatccaga cctgcccgat gatctcggcg gccgtgaggt cgcgctggaa
    51301 gccctgcttg ccggtggagc agaaactgca gtcgagcgcg caacccacct gcgaggacac
    51361 gcacagcgtg ccgcgcccgt cctcggggat gaacaccgtc tcgacacagc tgccgccggt
    51421 gacgcgcacc agccacttgc gcgtgccgtc ggcggagatg tgctcctcgg ccacctccgg
    51481 cgcgcgcacc tcggcgcact gcgccagccg ctcgcgcagc ttgcggctga tgtcggtcat
    51541 ctgctcgaag gaatccacgc cctggtggta gatccacttg aacagctgct gcgcccggta
    51601 gggcttttcg cccagcgcga ccaggaactc ctccatgcgc gcgcgcggca tgcccagcag
    51661 gttgacccgt gccgggggcg tgtcgctcgc gatcagggcg gcggagtttt ccatggacga
    51721 cagcgccgac gcctcaggcg cgatcgcaca gctcggtggc ggcgaagaaa tacgcgatct
    51781 cgcgcgccgc ggaagcggtc gagtccgagc cgtgcaccgc attggcgtcg atgctctggg
    51841 cgaagtcggc gcggatcgtg cccggggccg cgtccttcgg gttggtcgcg cccatcagct
    51901 cgcggttctt cgccacggcg ttctcgccct ccagcacctg caccagtacc gggcccgagg
    51961 tcatgaactc gaccagcgcc gggtagaacg gacggccctt gtgctcggca tagaagccgc
    52021 cggcggtggc gtcggtcagg cgcagcatct tcgcggccac gacgcgcagg ccgttcttct
    52081 cgaaacgcga gaggatttcg ccgacgacgt tcttcgccac ggcgtcaggc ttgatgatcg
    52141 aaagggtgcg ctcgagggtc attcggtact ccggatcgtg aaaagggtgc ggcagccgcg
    52201 ccgcaccggc caaaaaggag cggcattata cgcaccgcgc ggcccccccc ggtaaggggc
    52261 atcaggccgc gataatgccg gctttcaggc cagcatcccc ggctccggca ccgcgctgcc
    52321 ccgcagccgc gtcacggccc ggcggatggt atcggccgcc agcgcgacat cttcatcggt
    52381 gctgaagcgc ccgaggctga agcgcaatga cgcatgcgcc agctcgtcgc ccagtcctat
    52441 ggcacgcaaa acgtgggaag gttccaggct ggccgaggtg caggccgaac ccgaggacac
    52501 cgcgatgtcc ttcatcgcca tgatcatcga ctcgccctcc acgccggcga accgcacgtt
    52561 cagcgccccg gccacgcgcc gtgccgggtc gccgttctgt cccacgcccc ccaggtccgc
    52621 gatggacgcc cacagccggt cgcgcagcgc cgcgatgcgc ccggcctcgg cctgcatttc
    52681 ggcgcgcgcc aggcgcgccg cctcgcccat gccgacgatc tggtgggtgg gcagcgtgcc
    52741 ggaacgcatg ccgcgctcat ggccgccgcc gtgcatctgc gcctccagcc agacccgcgg
    52801 caggcgccgc acgtacagcg cccccacacc cttggggccg tacatcttgt gcgccgacag
    52861 cgaggccagg tcgaccggaa gcgccgcgag gtcgatcggg agcttgcccg cgccctgggc
    52921 ggcatcgacg tggaacacga tgccgcgctc gcggcacagc gcgccgaggg cggcgatgtc
    52981 gttgacggtg ccgagctcgt tgttcacgtg catcacgctc acgaggatcg tgtcctcgcg
    53041 cagcagcggc gccagcgcgg cgggctgcac gatgccgccg gcgtccgggg ccacgtagct
    53101 cagctcgaaa ccctccgtct ccagctggcg gcagctgtcg agcaccgcct tgtgctcgat
    53161 cgtcgaggtg acgatgtgcc gccccttgcg ccggttgaag tgcgctacac cctttagcgc
    53221 caggttgtcg gcctcggtcg ccccgctggt ccagacgatc tcgcgcggat cggcgcccac
    53281 cagctcggcc acctcgcgcc gcgcctgctc cacggcctcc tcggcgcgcc agccgtagat
    53341 gtgcgagcgc gaggagggat tgccgaaatt gccgtcgatg gtcaggcatt gcgccatgca
    53401 cgcggccacg cgcggatcca ccggcgtggt cgcgaggtag tcgaggtaga cgggcaggcg
    53461 catcgcgggg agtggccggc aggctctcag ttcagcgcgg tgacgctgat atcgcccgcc
    53521 acgcgctcgc gcgagccgtg ggtcccgcgc tcgtgctggc gccgcgagac ccgctgcaca
    53581 tcgctgcgcg cgaccagttc gcccagcgtg atgccgttga ggaaggaatg gatctgttcg
    53641 ctgaggtcgt tccacagcgt gtgggtcagg cagatttcac cctgctggca gccgcccttg
    53701 ccctcgcagg ccgtggcttc gtagctctcg ttcacggcat cgatgatcga ggcgacgctg
    53761 atctcggctc ccggacgctg caggcgatag ccgccaccgg ggccgcgcac gctgctcacc
    53821 agcccgctgc ggcgcaggcg cgcgaacagc tgttccaggt aggagagcga gatttcctgg
    53881 cgcccggaga tatcggacag gctcaccgga cccccgccct cgtgcagcgc gagatcgagc
    53941 atcgcggtca cggcataacg tcccttggtg gtcaggcgca tcgtgggttc ctggcgtgga
    54001 ttcgggtccc agtattcgta ttccgagtgt ggcagtcaag aattgggcgg ggatgcgcgc
    54061 tgcgcctcgg cgcgggtctt gccgatctcg gtgaggatcc cgcgcaggat attgagctcc
    54121 atctggtcca tccgcacgcg gttgaacaac cggcgcaggc gcgtcatcag ctggcgcggc
    54181 gcggccggat tgaggaagcc gatatcggcc agcgtcgcgt cgaggtgctc gtagaaacgc
    54241 tccatgccct cgacggtcgc gagcggctcg tcccagtccg gcgccgtcag cgctccctgc
    54301 tgctccagcg cggtcatgcg cagctcgtag cacagcagct gcaccgcggc ggccaggttg
    54361 agcgagctgt agtcggcgct ggtgggcacg tgcacgtgcc agtggcacag ccgcaactcc
    54421 tcgttgctga gcccggagtc ctcgcgcccg aacaccagcg cgatctcctc gccgcgacgc
    54481 tccccgaggc acagcggtcc cagctcgcgc ggcccgagct gcggcagctg gatgcgccgc
    54541 tcgcgcgcgc tggtgccgat caccaggctg cagtcggcca cggcctccgc cagcgaggcc
    54601 gtgaccaccg cggattcgag caggtccacg gcctgcaccg cgcggtaggt ggcctcgcgg
    54661 tgagggaatt cgaccggggc ggcgaggtac aggcgccgca accccatgtt cttcatcgcg
    54721 cgcgccgccg cgccgatgtt gcccgaatgc accgtgccca ccagcacgac gcgcacccgc
    54781 gcgagcaatt gcccgattgc ggcctcgtcc gtcgtctcgc tcattaccgt cccttgcgtc
    54841 cgaaagcgtg cattttgcgc ggcccgttcc gtcctgcaaa gctccgcgcg gccgtgctgc
    54901 tataatcgcg cgcccttttc ggggtccgca caaggagctt cccgcccatg catcccatgg
    54961 tcaatatcgc actcgcggcc gcccgcaagg caggcgagat catcgcgcgc gcggccgacc
    55021 ggctcgacat gatcgaggtg ggacgcaagt ccggcaacga tttcgtgacc gagatcgaca
    55081 aggccgccga gcgcgagatc atcacccagc tgcgcagggc ctaccccgat cacctgatac
    55141 tcgccgagga gagcggcgcc tcgggcgacg ccgcgcgcgc cggcggttac cagtggatca
    55201 tcgatccgct ggacggcacc accaacttca tgcgcggcat cccgcacttc gcggtctcga
    55261 tcgcctgcgt gcgcgacggc cgcctcgagc acgccgtggt cctcgacccg gtgcgccgcg
    55321 aggagttcac cgccacgcgc ggccagggcg cccagcgcaa cggacgtcgc atccgcgtca
    55381 gcacgcgcgc cggactcaag gaaggggtgc tcggcaccgg catcccgttc tgcgaaccct
    55441 cgatccgcca cctcccggcc tacctccaga ccctggagca gttcgcctcg cacaccgccg
    55501 ggatccgccg cgccggcgcc gccgcgctcg atctcgccta cgtcgcggcg ggacggctgg
    55561 acggtttctg ggaaatgggg ctcaagccct gggacatcgc cgcgggcgcc ctgctggtgc
    55621 aggaagccgg cggcctgatc agcgacttcg acggcggcaa tgactggatg gagagcggcc
    55681 gcatcgtggc cgcgaacccc aagtgcctca aggcgatgct gcaggtgatc cgcccggcgc
    55741 tcggcagcgc cgcgtaaccg gtagaaacgg acggctcagg gcgtttcctg ggcctcgagc
    55801 ttttccgggg ccggcagcag caggtccttg cggtccaggc cgagccacag cgaaaacgcc
    55861 gaggcgatgt agatcgtcga ataggaaccg gcgatgatcc ccacgaacat ggccagcgag
    55921 aaaccctgca gttgcgggcc gccggccacc agcatcgcga ccaccaccaa ctgcgtcaac
    55981 gcagaggtcg tgatggtcct gcccagcgtc tgggtcagcg acacgttgca caccgtgaaa
    56041 cggtccaccc ggcgcatctt gcggaagttc tcgcggacgc ggtcgtagat cacgatcgtg
    56101 tcgttgatcg aatagccgac cagcgacagc accgcggcca gcgccgcgag gtcgaactcc
    56161 cactggaaga tcgagaacag gcccaccgtg accagcacat cgtggaaaat cgccacgatc
    56221 gcgccggtcg ccatcttcca ctcgtagcgt atcgccacgt agccggccac cagcgccagt
    56281 gcgagcagca gcgcgagccc accctggtcg cgcagctcgt cgccgatctg cgcgcccacg
    56341 acgtcggtgc gcttgagcgt cgcgaccctg gcgccgccca cggcggccag cacctgctgc
    56401 ccgaccgttg cactgtcgcc cggcggcagg cgcaccagca cgtcgcgctc ggagccgaaa
    56461 tggaccacgg aggcggactc gaacccggcc tgcgcgagct gcccgctcac cgtggccgtc
    56521 tgcacctcct cgcgaaagcc gagctccacc acggtgcctc cggtgaagtc gagcccgagg
    56581 ttgagcccct gcaccgccag cgagatcagc ccggccaggc acagcgcggc cgacagcccc
    56641 gccgtccacg cgcgccactt gaggaaatcg atgaacttcg gttgttcgtc ttgcatacgc
    56701 ggtactttcc tagatcgaga tcttcgtgag gcgacggccg ccgtagacca ggttcgccag
    56761 cgcccgcgtg accaccaccg cggtgaacat cgaggtcatg atgccgatcg acagcgtcac
    56821 cgcgaacccg cgcaccggcc cggtgccgat ggcgtagagg atcacgccca cgatcagggt
    56881 cgtgaggttg gagtccatga tggtcagcca ggcgcggtcg tagccggccc cgatcgccga
    56941 gtgcggcggc atgccgttgc gcagttcctc gcggatgcgc gcgttgatga tgacgttcgc
    57001 atcgaccgcc atgcccaccg tcagcacgat gccggcgatg cccggcagcg tcagcgtcgc
    57061 cgacagcagc gacatcgccg cgaccagcag cagcacgttg ctgatcagcg ccaccaccgc
    57121 gaacacgccg aacatgcggt agagcaccag cgtggcgatg aacaccagcg ccgccccgat
    57181 catcagcgac tgcacgccca tgcggatgtt gtcggcgccg agcgacggcc ccacggcgcg
    57241 ctcctcgacg aaatcgagcg gtgccgccag cgcgccggaa cgcagcagca gcgcgagctc
    57301 cgcggactcg gccgggctgt cgaggccggt gatgcggaac tgcgtgccca gcgccgactg
    57361 gatggtggcg aggctgatga tcttcttctc gtcgtagcgc tcgagcaccg gggtctcgat
    57421 gccatcggga cccgtctcgt aacccaccgt gcgggtcttg cgctcgagga acagcaccgc
    57481 catgcgccgc ttgatgttgt cgcgcgtggc gcggttcatc agcgtgccgc cctggctgtc
    57541 gaggttgatg ttgacctgcg ggcggccgtt ctcgtcgaag ctcgacgtgg caccggtcac
    57601 gcggtcgccg ccgatgatca gctcgcgctc gagccaggcg ctgcgctcgc ccgagcgctg
    57661 gtcgcggaac tggaactgct ccttgtcgga gctcgccgcg tccgggcgcg cctccaggcg
    57721 gaactcgagg ttggcggtct tgccgaggat gcgcttggcc tcggcggtgt cctgcacacc
    57781 gggcagctcg accacgatgc ggttgcggcc ctgcttctgg atcatcggct cggccacgcc
    57841 gagctcgttg acgcggttgc gcagcgtggt gaggttctgg ttgaccgcgt agtcctccat
    57901 ctccttgatc tgctgcgcgc cgagcgtcag ttgcaggagg aacttgtcgc cgcgctcctc
    57961 catcacgcgt tgcagctcgg gcatcgcgtc ctggatcacg ccgaccgcgg cggtgcgcgt
    58021 cgcggcatcg gcgaacgcgg cctcgatgcg attgcccccg accaccctga ccagcccgcg
    58081 cacgcgtgcc tcgcgcagct tgttcttgag atccgactcg tagaccgaga tgcgcttggc
    58141 gaccgcggca tcggtatcga cctgcagcag gaaatgcacg ccgccgctca ggtcgaggcc
    58201 gagcttgagc ggcgcgccgc cgatcgccgc cagccacgcc ggcgtggtcg gcgccaggtt
    58261 gagcgccacc acgtaatcat cacccagcgc gcgcttgatc accgactgcg cgcgcagctg
    58321 gtcctcgatg ccggtcaggc gcagcagggc cgaatcggag tcgaactcgc tgccgaccgt
    58381 cgcgatctgc ttgtcctcga gcgccgcgag cgccttgtcc atcaccggct ggccgatctc
    58441 cagcgcgctg ctcgcgccgg agatctggac cgccggatcc ggcgggtaga gattcggcag
    58501 cgagtaaatg gcgccgaaca gcagcgcgaa cgcgaccagc aggttcttcc agaggggata
    58561 tcggttcagc atgcgcggac agggatccat ggcgtggatg cgacgacccc ggcatgtgcc
    58621 ggggcccgaa ggcgcgggat tatagccacg agaagcaggc gcaacaaatg cgccgcctca
    58681 gcccagccag gcgcgcgcgt tgcggaacag gcgcatccag cccccgtcct cgccccagcc
    58741 cggcgcccgc caggagttct gcacggtgcg gaacacccgc tccgggtgcg gcatcatgat
    58801 cgtcacccgc ccgtcgctgc tggtcaccgc cgccacggcc cccgccgaac cgttggggtt
    58861 ggccgggaag cgctgcgtcg ggttgccgcg gctgtcgacg tagcgcaacg ccacgcggcc
    58921 gtcatgctcg aggcccgcga gcgcgccggg cagaaattcg gcctgtccct cgccgtgcgc
    58981 gaccgacacc gggatcatcg acccctccat gccgcgcagc aggatcgagg ggctcgattc
    59041 cacgcgcacc agcgccaccc tcgcctcgta ctgctccgag gcgttgcgca cgaagcgcgg
    59101 ccagtgcgcg ctgcccggga tgatgtcgcg cagcccggac atcatctggc agccgttgca
    59161 gacaccgagc gtgaaggtgt cgccgcgcgc gaagaagccc tggaactgcg tgcgcgcgcg
    59221 ctcgttgaac aacaccgtcc tggcccaacc gccgccggcg cccagcacgt cgccgtagga
    59281 gaagccgccg caggccgcga tgccgctcac cgatgccagg tcgagcgcgc ccgagagcac
    59341 gtcgctcatg tgtacgtcga tggcctcgaa gccggcgcgg tcgaaggccg cggccatttc
    59401 gtactggccg ttgacgccct gctcgcgcag caccgcgacg cgcggacgcg cgccagtggc
    59461 gatgaacggc gcctcgatgc gctcggcggg gtcgtaggcg agccgcgcgc cgaggcccgg
    59521 atcgtcggca tcgagcagcg cgtcgtattc ctcgcgcgcg cagtcgggat tgtcgcgcag
    59581 cgcctgcatg cggaagctga gctcagccca ggcgcgatgc agcgcggtgc gcgagttctc
    59641 gtagaccacg cgctcgccgt tgcggatgcg cacggtgtcg ccgcgcgcgg ggcgccccag
    59701 cacgtgcgcg tacgccccga gaccggccgc cgcgaaatac gcccgcaccg ccgcgaggtc
    59761 ggcatcgcgc acctgcatca ccgcgcccag ttcctcgctg aacagcgcgg cgagcgcggc
    59821 ggcgccgagc gcggcgatgt cgacatcgag cgccgcccgc ccggcgaaag ccatctcgca
    59881 cagcgtcacg aacagcccgc cgtcggaacg gtcgtggtag gcgagcagca ggccctcggc
    59941 gatgcagcgt tgcagcgccg cgaagaaacc gccgatcagc gcgggatcat cgagatccgg
    60001 cgcgctgtcg ccgacctgct tgtagacctg cgccagcgcc gaggcaccga ggcggttgcg
    60061 cccgcgcccg agatccacca gcagcagctg cgatgcgctc tcgtcgcgac ggatttccgg
    60121 cgtcaccgcc aggcgcacgt cggtcaccgg cgcgaaggcc gagacgatca gcgacagcgg
    60181 cgcggtcacg gccctcgccg cacccgcctc gctccagcgc gtcgtcatgg acatcgaatc
    60241 cttgcccacc gggatcacga tgccgagcgc cggacacagc tccatgccca ccgcgcgcac
    60301 cgcctcgaac aacgcctggt cctcgcccgg gtggccggcg gcggccatcc agttcgcgga
    60361 gagtttcacg tcggcaaggc gcgcgatcgg tgccgaggcg atgttggtca gcgcctcgcc
    60421 caccgccatg cgcgccgagg cggcggcatc gatcagcgcg agcggcgtgc gctcgcccat
    60481 cgccatcgcc tcgcccgcgc aggtgtcgaa gctcgtggtc gtgatgccgg catcggccac
    60541 cggcacctgc cagggaccga ccatctggtc gcggctcacc tgccccgtga cgctgcgatc
    60601 gccgatggtc accaggaagc ccttgcccgc cacggccggc aggcgcagca cgcgctcgac
    60661 ggcgtccgcg atatccacat gatccagcgc gagcggcacg cgcgcgggca cggtgcgcgc
    60721 gaactcgcgg tgcatgcgcg gcggcttgcc gaacagcacc gacagcggca agtccaccgg
    60781 ccgcgagccg tcacgggcgt cggtcagcgc gaggtgcgcc gccgcgctcg cctcgccgac
    60841 caccgcatag gggcagcgct cgcgctcgca gatcgcggcg aagcgatcga gatcggcggc
    60901 gtccaccgcc agcacgtagc gctcctgcgc ctcgttgcac cacagctcga gcggcgagag
    60961 cccggtatcg gcgctgggaa tcttctcgag cgcgatgcgc ccgccgcagc cgccgtcctt
    61021 caccagctcg ggcagcgcgt tggaaagccc tccggcgccc acgtcgtgga tgaagcggat
    61081 cgggttgcgc tcaccaagct gccagcagcg gtcgatcact tcctggcagc ggtgctcgat
    61141 ctcggcgttg tcgcgctgca ccgaggcgaa gtcgagctcc tcgtggctcg cgcccgaagc
    61201 catcgaggac gccgcgccac cgcccagccc gatcagcatc gcgggcccgc cgagcaccac
    61261 caggcgcgtg ccctcgccca tggcgccctt gagcacgtgc ccctcgcgga tgttgcccag
    61321 gccgccggcg accatgatcg gcttgtgata gccgcgcacc tcgccctcgt gcacctcctc
    61381 gaaactgcgg aagtagccgc agatcgcggg acggccgaat tcgttgttga acgccgcgcc
    61441 accgatcggg ccctcgagca tgatctcgag cgccgaggcg atgcgcccgg gcttgccgtg
    61501 atcggtctcc cagggttgcg cgaattgcgg cacgcgcagg ttcgacacgc tgaagcccac
    61561 gaggccggcc ttggggcgcg cgccgatacc caccgcgccc tcgtcgcgga tctcgccgcc
    61621 cgatccggtg gccgcacccg ggtacggcgc gatcgcggtc gggtggttgt gcgtctcgac
    61681 cttcatcagg atgtgcacag gctcggggtg atacgcgaac acgcgcgttt cgggatcggg
    61741 aaagaagcgt cccgcttcgt gcccgcggat cacggcggcg ttgtcggagt acgcgctcag
    61801 caccgccgga tccgcggcct gctcgtgcgt gttgcggatc atgccgaaca ggctgtgcga
    61861 ctgcggctcg ccgtcgatgc tccagctcgc attgaagatc ttgtggcggc agtgctcgct
    61921 gttggcctgc gcgaacatca tcagctccac atcggcggga ttgcgtccga gctcgacgaa
    61981 ggccgcgcac agataatcga tctcgtcatc ggcgagcgcg aaaccgaact cacgattcgc
    62041 gcgctccagc gccgcgcggc caccgccgag aatatccacc gtgcgcagcg cgcgcggcgc
    62101 ctcgtgcacg aacagcttcg ccgcgtcctc gaggctcgcg aacaccgcct cgaccatgcg
    62161 atcgtgcagc ggcgcggcga tgcgcgcgcg taactctgca gcaatcgtgc cttcgcaatt
    62221 cacgtaatac gcgatgccgc gctcgatgcg ccgcacgcgc gccaggccgc agttgcgcgc
    62281 gatgtcggtg gccttgctcg accacggcga caacgtgccc gggcgcggta ccacgagaaa
    62341 caattcgccc gcgaattgtt gcgcctcgcg atgcgggccg taatcgagca attgccgcag
    62401 catcgcgctc tcgtcgtcgc gcgcttctcc gtcgatctcg atgaaatgca tgaatcgcgc
    62461 ctgcacatcg cgcacatcgg cggcgatcga ttgcagttcg ccgagcagcc ggcgaagacg
    62521 gaatggcgaa agcgccgaag cgccggcgag agtcagcatg actgttcccg aaaattgaaa
    62581 ttgaatgaaa tgttgtgcct cgagcgcaga attctaccgt atgccccgaa gcgccagtta
    62641 gctgcaattg cgtacgattt tgacatcaac attcgcgcga tggcgtacaa cacaccgata
    62701 gcgattacta acattcgtgc gcacgcggaa aaacgtaccg cgaaatagcg gtaatttttt
    62761 ttgtccgatt gggttcccat cgcgcgattg gcgactagaa tcgacccccg ccaggtacag
    62821 caagagtgtg acctatgtgg atcacagcaa ctactggaac actggaaagc aggccacggc
    62881 ccatcacgaa cgtcctgaaa ggcgcattga tgggcattct gtgtgcagtt ctcgccgcgt
    62941 gtcccagcga gaccattcag tcacactccg gcccgcttcc acgagattcc ctcgacgaag
    63001 tcctggcgcg cggcaagctg cgcgtcgtca gccgggtcaa cccggcgacc ttcgtcgtag
    63061 acaagcacgg cccttccggg atcgagttcg agctcgcgcg cgcgttcgcc gagaaaatcg
    63121 gcgtcgagct cgagatgatc ccggcggcca acatcgccga ggtctatgcc gcgctcgaca
    63181 gcggcacggc ggacatggcc gcctcgggcc tgtcggcgcg cctttccggc acctcgagct
    63241 actggtacag ctcgccctac ctcgaggtgc gccagcagat cgtctacagg agcggcgcgg
    63301 cccgcccgcg ctccgtcgcc gacctggtgg gcaagcgcgt gatggtgctc gcgcagagca
    63361 gctccgccga cgcgcttgcc gaggcgcagc gcgcgcatcc cggcctcggc tggatggaag
    63421 ccacgcgcat cgagaccttc gagctgctgc gcaagatcat ggacgacgag atcgattacg
    63481 cgatcgtgaa gtccaatgaa ttcgagatgc acgagggcct gttccccggc atcgagatcg
    63541 ccttcgagct cgaggagcgc gagcagctcg cgtgggccac cgccgagcgc ggccgcaatg
    63601 cgcgactgca ctcctcgatg cagaatttcc tcgcgcgcta cgaggaatcg ggcgagctgg
    63661 acctgctgcg cgagcgcttc ttcggttacc tgcccgagat caaccgcaag gcgctgaccg
    63721 cgtttgccga gcgcgtcgaa tcgcacctgc cgcgattcga gggggtgctg cgcaggatcg
    63781 gcgccgagga aggcatcgac tggcgcctgc tggccgcgat cagctaccag gaatcgcact
    63841 ggaatccgcg cgcggtgtcg tacaccggcg tgcgcggcat gatgatgctg acgaaggtga
    63901 cggcgcgcga ggtcggcgtg accgatcgca ccaacctcga gcaaagcctg cgcggcggcg
    63961 cgcgctattt cctgaaactg gtggagcagg tgagcgagga catcgcgccc tcggaacgcg
    64021 agctcttcgc gctcgccgcc tacaacatcg gccccgcgca cgtcgaggac gcgcgccgca
    64081 tcgccgagat gcgcggcgcc gatccggacg tgtgggccga cgtggagaag cagctgccgc
    64141 tgctgacaaa gcgcgcatgg cattcgcgca cccgccacgg ctacgcgcgc ggctacgaaa
    64201 ccctgagctt cgtcaaccgc atccgccagt accaccgctt cctcgagcac cacgacgagg
    64261 aatcgaacat gctgcaggtc gcgcagaacg cgcgcacctc gttcgcgcag ctgcccaccc
    64321 gctcctgacc acacaaaaat gtgggtcgcc attcatggcg acatccggcg gcggcggaag
    64381 aaatcccgca tcatccgcgc cgcatcgtcc gcgcacacgc cctcgctcca cgccacgcga
    64441 tggttcagaa aagccgcgtc cagcagcgcg aggttgctcg ccaccgcgcc ggcgcgcggc
    64501 tcgcgcgcgg cgaacaccac gcgcgccacg cgcgcatgca ccagcgcgcc cgcgcacatc
    64561 gcgcagggct cgatgctgac gtacagcgtg gaaccgctca gccggtagtt gctcaacgca
    64621 ttcgccgcgg cgcgcagcgc cacgatctcg gcgtgcgcgg tgggatcgca gcgcccgatc
    64681 ggcgcgttcc agccctcgcc gagcacctcg ccgtcgcgca ccagcaccgc gcctacgggc
    64741 acttcgcccg cgccctcgcc cgcgcgcgct agctcgagcg cgcggcgcat ccagtgctcg
    64801 tcttcttctg cgctcgtcat gtgcgttctc ccgtcgtggc gccagcgcgc gcgcccgcga
    64861 tgatagccca gctcgacgcg gcaaccgcgc cgcgcagccc ctgcaaccgt cctcaggccg
    64921 gcagcggcaa ttcgccctcg ctcaggaagg tctcgaggac gaagcggacg acggcgctcg
    64981 cgcccacctg cttgaacgcg acttcgcgtg cgccggcctc gcacagccag cgcgtggtct
    65041 cccgcggcgc cggctcgctg aaagcggcgg gcgaaatcag cccgacgcac aagccgtgtc
    65101 ccgggtcgcg cgccgacggg tactcgaagg cctccacgcc cgcctcgcgc atcgcggtcc
    65161 ccagcagctg cgtctgcacg taatccttcg ggtgcgcgat cgtcgcggcg aaacgcgcga
    65221 acggtgcctc gtgcagcttc accccgcgcg ccgtccggta gagcgccgag aacagcgtgt
    65281 gctgcgaacg gatggccgcc ttcggcggcg ccgcgtccat cgagtaccag aacacgaagc
    65341 ggtaataggc ggcctcggcc agcgtggcat ccgtgccgca agcgccgtag aaaatgcccg
    65401 gctcgtgcac gcgcccgaag cgcgagcccc acgacagcgg cggatagcga aacggcgtct
    65461 tcagcaggta gtgcaggcct tcggtcccgg cacggtacgg gggctttacc tcgtccagca
    65521 tcgcttccag cagcgcctgc tcctcgaggg tgtcgacgta gccgagcgtc gcgacctgct
    65581 cctggctctc gacgaggcga tacagcgtgc ccgccacgcg gcgaatctgc gcggcgccgt
    65641 ggcacgcctc ccagatcgtc acaccttgcc ccgcagggca tcgacgaact gcagcacggt
    65701 gaccaggccc tggatgctct cgatctgccg cgccgggacg ccccccgtcg ccttgttcgc
    65761 gctgcgcatg aagtgccgga tccagtcctg gtcgccgccg gtgagcgcgt acagcgcccg
    65821 cgccacgcgc acgaggagca acgccagctc gcccgccttg gaccgcggat ccagcgcggg
    65881 ttgctgtttg agccggctga tcgccgtgcg gtgcatgccg agcacggcgg ccagctccgc
    65941 ctgtttcagc ccgagctgcg ccgcggcgct cagcacggcc ttggccagca cgacctcggg
    66001 cgcggcttgg gggtgcggct gggtggacat gcgcggctcc cggggaaaat gctatctgca
    66061 catatgctat gcacgatgtg cggatagcac aacccgtaat tgccccgctc agccgccgat
    66121 cgccgcgact tccgccggcg ccaggtgccg ccattcgcct ggcgcgagcg ccgcatcgag
    66181 cacgatcccg ccgaccgcga gccggtgcag cgcgaggacc tcgttgcgga agacgccgaa
    66241 catccgcctg acctggtggt agcggccctc gtggatcgtg agctccgcgc gggtggcgga
    66301 cagggtcacc agccgcgcgg gctgcgtcac caggttctcg tagcgcagat agatacctgc
    66361 cgcgaagcgc tcggcgtatt ccggcgtgat cgggtcgcgt gtatgcacga ggtaggtctt
    66421 gggaatcttg cgctcgggca gcgtgatgcg gcgcgtccag ttgccgtcgc tgctgagcag
    66481 caacagcccc gtggtattga agtcgagcct cccggcgagg tgcagttcct cacgcgccgg
    66541 caggtcgatc agatcgagca ccgtacagtg cttgcggtcg gtggtggcgc tgacgcagcc
    66601 gcgcggcttg tgcagcatca ggtacacggg ctcgcgcgcg cgcaacagcc gtcccgccac
    66661 ctcgacgcgg caaaaggtcg agaccgcctg gctgccgtcg gtggccggca cgccgttcag
    66721 cagcacctcg cgcgcggcga tcatgtgctg cgcgctgcgc gccgagcacg ccagccggga
    66781 gcctatgaag cggtcgagtc gcatcggggt tgatctacct cgcgtagttc gcggctgttg
    66841 ccgaagcatc cacggcgcct tggtgtggat gcgcccaaga attcatcgac tatcgtagcg
    66901 cggctgcgcc gttccatgga gaaccatgga tgagctcgac aaccgccccc gttgttgcca
    66961 acatcgaccg acgagagcat ccagaatcca gtcaatgccg tctgcaaaca catcggccga
    67021 gttattccgg cacgtcagcg ccgagaaagc cggcatctac cgcagcatca tggcgagttt
    67081 cgccgcggca aagcgccagt tccggctgca catgcgaccc gacgaagtgc tgaccgaggc
    67141 tcggtggacc gatggcgcac caaagctcga ggaagttcag gccgcgctcg ctcaactcgc
    67201 cgaatggggc aacctcgagg cgcagcccga cacagcacgc gtcgccagca tcagcgactt
    67261 ttaccgcgcc cgcttcctct accggctctc ccatggcggc gatgcggtcg agacagccct
    67321 cgcggccttc gccgacgcac tcgaacggcg cgccgaactg cagacgatcg cgctggagga
    67381 catcgccagc cggctcagga ccctgctcgc gctggccgac gcgccggcga tcgatgcggc
    67441 gaagctgcac gagacgctgc gcgatctggt gcgtgtgttc gagggcctgg ccgacaacgc
    67501 ccaggcattc atggccggca tggcccgcag catcgagctg caacaggccg atgccaccgc
    67561 ggtggtggct tacaagcaac ggctgatcga ctacctcgaa cgtttcatcg gcgatctggt
    67621 cgggcgctcc ggcgcgatcg cagagcacat tgccgcgctg catccgcgca tcgatccact
    67681 gctgtggtcg gccgcgcaac gcgaggcgcg cgacgccgct cccgctgacc aacaagcgca
    67741 gaccgaggcg ctcgcacagc gccagcaggc gtggcgtgaa cgctgggggg gcctgcgcag
    67801 ctggttcgtc agcgcccatc atgaaccgcc tcaggccgag gtgttgcgct cgaaggcgcg
    67861 cgcggcgatt ccgcagctgc tggcggcggt cgcggcgctg aacgagcggc gcagcgggcg
    67921 cagcgaccgc tcggccgact tcagggtgct cgccggctgg ttcgcgagct gcgcgggcga
    67981 tgacgactcg caccgcctcg gtcgcgccgc cttcgccctc aacccggcgc ggcatttctc
    68041 gttgaatccg gacgccggca acgaactgcc ggccacgacg ccgtgggccg aggcgccgcc
    68101 gctgcgtatc cacccgcgcc tgcgcgagta cggcgaggcc gctccacgag gtccgctgcc
    68161 cagggtgcat gaacgctcgg aggaacgcgc cctgctcgcg cgccaattgc gtgaagaaca
    68221 tctgcaggtc gaagccgcgc gtgcacgcct ggccacgggc cggatgacgc gcctgtccga
    68281 cattgggcgc ctcgatccgc acgccttcgc gctgttgctg tcgctgctgg gcgaggcgtt
    68341 gagcgcgcaa tcctctcccg aggacgtgat cgagcgccag accggcgatg gcctgctgcg
    68401 gattcgtctc gagccgatgg cagcacacac gcgggccgaa atcctcaccg atgccggcgt
    68461 ctttgccggc cgtgaccacc gcatcacgat caccgccacg catacgccct gatgagaagc
    68521 gacagcaccg cgatcggcca gcagcaggcc cgccatcaac gcgaggagat cgccgacgcg
    68581 ctgcgcgccc tgctgatgac gccgttgatg acgcctgcac atgccgagtt cgccgcggtg
    68641 cgccgccatg ccgatgcgct gcgcgaatgg ttcgtccgcg agaccggctg ggcgctgcac
    68701 atcgaacgcg acgcggcacg cctgtacaag cgcccggccg acctgaacga cgccacccgc
    68761 gggatgcccg gctacgagcg ccgccgctat gtgctgctgt gcctcgcctg cgcggtgctg
    68821 gagcgtgccg acccgcagat cacactgcac gtgctcggcg accggctgct cgcccttgcc
    68881 gcggaaccgg cgctggccgc gctcggcttc agcttcacac tccaggcgca gcacgagcgg
    68941 cgcgagctgg tcgcggtgtg ccgcacactg ctggagctcg gcgtgctgca tcgcgtggcc
    69001 ggtgacgaag agggcttcgt gcgcggcggc aacgcaggcc cggacagcct ccacgacgcg
    69061 ctctacgacg tacagcggcg ggttctagcc ggtatgctcg cggcatcgcg cggcccttcc
    69121 acatggcccg cggacgaagc cccggccacg ctcgaggcgc ggctcgccgc tctggttact
    69181 gagcccgttc cagacagcga cgaaggccga cgtacggcgc tgcgccacca cctggcgcgc
    69241 cgcctgctgg acgacccggt gatctacctc gaccgcctcg acgccgacca gcgcgcctac
    69301 ttcctgaacc agcgcggcac catggccatc cggctgtgtg acgccaccgg gctggtcgcc
    69361 gaacagcgcg ccgaggggct ggcgctggcc gacgaggacg gcgaactgac cgacgtggcg
    69421 atgccggccg agg
//
LOCUS       DAORUQ010000002        50710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_384_length_50710_cov_6.230797, whole genome shotgun sequence.
ACCESSION   DAORUQ010000002 DAORUQ010000000
VERSION     DAORUQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 50710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50710
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_50710_cov_6.230797"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLW13_00325"
     CDS             complement(<1..1293)
                     /locus_tag="PLW13_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY68840.1"
                     /translation="MNTKADIILEDLSEPLTFPVDAYVSREYAEAEGGKLWARVWQHA
                     GRVEDIPEVGDYITYEIANDSILIVRAATGTIRAYHNVCPHRGRRLVSTPKGGNGARG
                     NATKQSFFCGFHGWSFDLQGSNTHILNKDDWKGTLTNERTCLAAVKVDTWGGWIWVNM
                     DPNCISLREYLEPAAGILDPFRFEDMRYRFRQWTVFDCNWKVALEAFMEPYHVAATHP
                     QLTKYGDFYAWSKVQGLHGNDGYDAPDHQDEGSAATTTVHRTGKGADARVMMGEMQRE
                     FWETVGASTTQVLVDAALRLVDELPEGTPADEVHRHWLASCKADYAAMGVEWPQISDE
                     QMATAGLAWHVFPNMGILQGPTFALCYRTRPYGTDPDKCIYEAYAIERYPQGQEPKTE
                     WTYADATEEHWRKVIAQDFSNMEMVQLGLKSRGFRGNLP"
     gene            complement(1315..3171)
                     /locus_tag="PLW13_00330"
     CDS             complement(1315..3171)
                     /locus_tag="PLW13_00330"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY68841.1"
                     /translation="MINTKSPGLPSAEEIDIPALKEKYRQERDRRIRKEGQEQYVRPV
                     DDFAAAYEADPYTPVIPRKAVCEEIDIAVLGGGWSGIAAGVHIRKSGLTSFRHIDHAG
                     DFGGVWYWNRYPGLQCDNDAYCYLPLLEETGFMPSKKFTDGHEIREYAQSIAKKYELY
                     ENALFHTMVESLRWDEELKRWRIATRQGDDIRARFVIMANGLLNIPKLPGIPGIHGFK
                     GHMFHTARWDYDYTGGCEKRPVLDKLADKRVALVGTGATAIQLVPYLGKYAKQFYVLQ
                     RTPSCVDSRRNSETDPEWVKTLKPGWQAQRRDNFHRGANERFLRGEKDLICDIWTEVN
                     RNLSAEQEEQGWPELTAQEFAARFEEMDFRVMERLRRRVDAIVEDKETAELLKPWYRY
                     QCKRPTSNDEYYQTFNRHNVKLIDVSETQGVQRMTQNGFVANGVEYEVDCMLFASGYE
                     VTSDLDRRWGIPVFEGRGGLSMYDNWNGSYKTLHGMMTHGFPNLFTTGYYQGGLNSTL
                     TYNFEQQVKHMLYIIKEVLDRGALAAEVSKEAQDAWIRHMRETEVNKAEWIASCTPSY
                     FNNEGKPDVDENGNERYRFYLGELYGPGWDAFMRVLENWRSRGDLDGLVLSR"
     gene            complement(3316..4155)
                     /locus_tag="PLW13_00335"
     CDS             complement(3316..4155)
                     /locus_tag="PLW13_00335"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016981957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY68842.1"
                     /translation="MTTATATLEALHATDLSPLIGSKVHLDKAALLSGEYAADLRELL
                     ERRGVVVFPQINFNDEEQVAFTRTMGRFLPELEGQEVYNITLDKSLNQKSEYLKGSLY
                     WHADGTMNKVPIGVAILTSKVLPTWGGNTEFCNTYAAYELLPDEEKKQIEGLRAMHSA
                     WTSLFYYEPEPSYAKLEEMIRIGEVELPLVWKHRSGRKSLVLGCTAHHVIGMSPTQST
                     RLLVRLRDWATQPRFVYSHEWSVGDAVMWDNTGTMHRAMPYDPDCGRLLHRTKTEGEE
                     PIV"
     gene            4338..5861
                     /locus_tag="PLW13_00340"
     CDS             4338..5861
                     /locus_tag="PLW13_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY68843.1"
                     /translation="MIQINDRASFNCLGCRRVARKQASTHADHPVFLQQENAMSAASP
                     ATIATDPARSPAAGTDGPSHWQTLSQGVGVGRYTDPEFTRLEFEKLWSRVWQVAARID
                     EIPEKGDYTTYKIGDQSILLVRVDESTIKAYYNFCPHRGTTLGHGCGHFDGGKIICPY
                     HGWRWDLEGNIKLVLERHEFCDGKLKDSDVAMREIHSRVFAGFVFINLDPNPESFDDF
                     IAPLRQFLGDIVLGDMRHIWWKRTVIPCNWKVAQEAFYEAYHVSATHPQLEKVGREVV
                     YGDRPDGPMMYRDLTYDAFAHGHGQFYGGDKTPMAGHVQEPKSTDDLLEEMAARMNLT
                     AEGLGAMILKEDIELLLTLRGKHLPPGTNLGAEYVRLQYETAAKSNRPMPKLTPEIIG
                     RWGGVTVAFPNVLVLMQAGSAALYRVLPHATDPNQCTFEIRSVKTYPAGVKTPRAIVE
                     DICDPEQVGLIPRQDLGNLPRVQEGLRSRGMKQAWLASNQEKLILNMHRELDRYLGS"
     gene            5921..6397
                     /locus_tag="PLW13_00345"
     CDS             5921..6397
                     /locus_tag="PLW13_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY68844.1"
                     /translation="MNHAELMARESIRHTMASYTMAGDRLRAEDFIAVFTEDAILETD
                     GVPAQDAFRHVGRQAIHDWIERWRTPAADDQKPVHQASFVRHHLATSLIELVDAETAN
                     ARTYWTAYTDIGPDHCGCYLDLFRKSGERWLIAHRRIRLDWRSPDSLMFTAIARTS"
     gene            6494..7402
                     /locus_tag="PLW13_00350"
     CDS             6494..7402
                     /locus_tag="PLW13_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY68845.1"
                     /translation="MLEGEKILITGATGKIAFPIARALARNNEVWGVARLQNAGDRDK
                     LASTGVEPVALDLSAGDFASLPDDFSYVFHAAVDPGQGDWCRCVRTNAHYSGDLLYHC
                     RSAKGFVFCSTGSIYGYQGQRPLTESDPPGVPLRANYSFSKVAAESVCTWIATHFGIP
                     LTIIRICSTYGPLGGTPVDRLEAILQRRPIRLHPDKPNNYNPIYEDDYVELGIRAMEV
                     AATPPLVVNWAGSETVSIEDYCSYMGELVGIAPVFEYTPAAHTPLWPDVTRMHEVLGR
                     TRVHWRDGMRRMIKALHPELVLKGEQ"
     gene            7404..8168
                     /locus_tag="PLW13_00355"
     CDS             7404..8168
                     /locus_tag="PLW13_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68846.1"
                     /translation="MNDHRDSSSAGFIGTPSDAVPQILAEGRGGITTLFVSMAQRHPD
                     GADAHYLRWHTLDHRPEQHRLASVRASLRVVSTPACRAARAASDERFEAIDHLMTYFF
                     ADIGGLDEFNKLSRALSSAGRVPFVLPPVQRGVYAVETRIAAPRAKVGSDVLPWCPAK
                     GVYLLIEEGAASATRLTDVPGVAGLWSASSVPTDFSTAGGGQQITYCFLDDDPVATAG
                     RLRRVLEQRWAVAGIQPLLAAPFYPVVPYEWDRYLP"
     gene            complement(8241..8684)
                     /locus_tag="PLW13_00360"
     CDS             complement(8241..8684)
                     /locus_tag="PLW13_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY68847.1"
                     /translation="MSGLPTVSISHIGICTSDVEHSLRFYIEALGFVLERSIDEIGPP
                     YDSLMELPGVRCRVHYVKCGAVTIELIGYPGAGVTGSAQRRAMNQLGFTHMTLVVDDV
                     NAAVERVVRFGGHVHRETAIDSPFGPMVFCTDPDGARIELVQSAG"
     gene            complement(8694..9104)
                     /locus_tag="PLW13_00365"
     CDS             complement(8694..9104)
                     /locus_tag="PLW13_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY68848.1"
                     /translation="MSEQNKQVVLTFIDAMGKGDAVAAAPCLAPDAFTLAKGFGKFAG
                     VRKHDTILATIAAFRKLVPDGMHPDIHSVTAEGERVVVEFDGNATLCNGESYCNQYCM
                     VFLLREGKIKQVNEYFCTILADKLLFPLVAAEGL"
     gene            complement(9187..9936)
                     /locus_tag="PLW13_00370"
     CDS             complement(9187..9936)
                     /locus_tag="PLW13_00370"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY68849.1"
                     /translation="MSQLLAGQVAIITGGASGLGRATVELFVEQGARVVIADVNAEQG
                     EALAAMLGAPAAFKRTDVADARQVQELVDFAVERFGGLHVMFNNAGISGAVHNRFLED
                     DLADFERVIAVNLFGVMAGCQCAGRYMAAHGGGSIINTTSIAGLKPGLPLISYRAAKA
                     GVIHVTKSIATDLGEYGIRVNCIAPGHIPAAQTFYDLSEMTRRVQPLRRQGTPRDVAN
                     AALYLASDLSAQVTGMVLPVDGGSNLGWSRK"
     gene            complement(9998..10813)
                     /locus_tag="PLW13_00375"
     CDS             complement(9998..10813)
                     /locus_tag="PLW13_00375"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY68850.1"
                     /translation="MTLQVIDLTPRVGSEIRTDVETLMSGSEAAIIRNILEQRGVVFF
                     RGLDIDDAQQVAIAETLGKTVASEGEGGIYKISLDKAVNQRADYLKGSLFWHFDGSLQ
                     PYPNLAALLRAIKLSATGGQTEFCNTYAAYDDLPDDEKKSLEKLRVVHSAARSQFYVN
                     PEMSYAELEMWQRAPSKSCPLVWTHQSGRKSLLLGATADYVEGMSVEESRALLARLRD
                     WATQPRFVYRHEWQVGDLLIWDNTGTMHRALPYPLDSGRLMHRTILAGEEPLL"
     gene            complement(10833..11156)
                     /locus_tag="PLW13_00380"
     CDS             complement(10833..11156)
                     /locus_tag="PLW13_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68851.1"
                     /translation="MSTLLPAKFAQLEHLVAEWAIEDGHARYVKRVNSSMEQIRAFYD
                     EIFPHAEAAVAYLDQFDYQQPLPPDAANLRNLLYSLITVSLAVELWKQPRVKHSARTI
                     LTRLS"
     gene            complement(11153..12439)
                     /locus_tag="PLW13_00385"
     CDS             complement(11153..12439)
                     /locus_tag="PLW13_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY68852.1"
                     /translation="MERDPIVTKPPSGHWSDAYPELGRGPVSLDDCVSREFYEKEREH
                     VFRKNWLYVGRVERLPRKGSYFTRELGFASTSIIVVRGKDDVIRAFHNICPHRGNKLV
                     WRDDPFEEVEGQSPALYCRFHGWSFELDGSLIAPTRKDLLLDFDPDKCRVPPVQCDVW
                     EGFIFINLNPDNTESVRDFLGELAHGIEGYPFAGPHQVYRFKAEIQCNWKIFMDGFAE
                     SYHGPYLHAASFGNLTAAAKAALEAPNPFTDALAYRLEGLHRMFSFAGEPSRKTPYSK
                     PIECVFEASAAGPWNKKIDVGSLPRGLNPTRSENYGFDSFQFFPNFVLIFGASGFTTH
                     VHWPTGPHSHIIEVEMFYRPPTTHKERLGQELTVTFLNDIILEDSSPLEGMQAMLNSG
                     AIREFHFNDEELLVRHLHKVVVDQVNAGEEKKGGGK"
     gene            12596..13546
                     /locus_tag="PLW13_00390"
     CDS             12596..13546
                     /locus_tag="PLW13_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY68853.1"
                     /translation="MGHNLPFDIDALLVFAKVVECRSLSKAASLLGMPKSTASRKISK
                     LESDLGIRLLRKNTHQITVTDLGEKIYGHALTILAEANDVRALVEGSQQEPQGVLRAA
                     IPVFVGIDFASRVGATFLQRYPKSRLEIRLVDNMVHPVKDGFDVVFGAGPLQDSTLIA
                     RKLFTLECFLCAAPDFIRTLPEPLTTPTQLNTLPFIDSGFYEEPRKLHLARGKKHYQL
                     SPLVRARANNFQISKQYILQGLGIGAMPKQIICSGELQEGSLVPVLPDWSLETVDVYM
                     IHPFQLSFSNLISAFYETAVEIITQNTERSGTLDTPPAPH"
     gene            complement(13696..16158)
                     /locus_tag="PLW13_00395"
     CDS             complement(13696..16158)
                     /locus_tag="PLW13_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY68854.1"
                     /translation="MSKSTRKGSRHVFHTAAMLPTVMLGAGLAVPVPEAHAAIEQQEE
                     VIVTARRKEESLQDVPISMTVFSQAMLDERNVISATDLVNHTPSLSANTRFGSDQASF
                     AIRGFTQEFRTTASVGVYFADVVAPRGGAVITAGDGAGPGAFFDLQNVQVLKGPQGTL
                     FGRNTTGGAIQLVPQKPTGELGGYAELSAGNYDMERAQGVINVPVSDRARARFGVDTL
                     QRDGYMKNVSGVGPNRFTDVDYIAGRASLSVDMTDAVENYTIATYTDSENNGTIQGLF
                     VCKPGGLLDAFCQPTLESQKDDFYTVAQGDVENPVSKLEQWQVINTTTWEVSDALTVR
                     NILSYADLEQTIRHANFGTNFRYPAAYAPLLPSPDLVGAPVTFYPSGPAGMPSSSQTT
                     FVEELQFSGRALDDRLDWQGGVYYENSRPDGWSGLLSTTFLTCPQLPEGGDPSFWQCL
                     DVFGAGFVQSSVGKIEYENKAAYAQTTYDISDEFSVTAGLRYTVDKTEGTNSQVIYTG
                     FPRAAPGAPASGPDCVLSPATGVAAPGCRQDLDKETDAPTWLIGLDYVPTPDLLLYAK
                     YARGYRQGSVNLFAAEGIQDFDAEEVDAYELGGKWRFDAPLPGTLNVALFHNDLSDQQ
                     LQVGYSSSTGEAAFTTGIVNAGSSTIQGAEIEATLEFHDGLMFSLSYTYLDTELDETE
                     LPQPASDSPYDEVTGYVEAGSALSFSPEHTATAALNYLLPLPADVGDVSIGASYTYID
                     EQLAIVSTYGWLDSRKLLNLNAGWKGIMGSAFDVSIFATNVTDEEYPVNITGFYDNPS
                     IGADFRVTGEPRMYGARLRYNF"
     gene            complement(16210..16671)
                     /locus_tag="PLW13_00400"
     CDS             complement(16210..16671)
                     /locus_tag="PLW13_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68855.1"
                     /translation="MALQEVSDVIGNYTGLSRTVLEYSQVMKRLVDSAGRPGFSVASW
                     APLGELIAIDEFERVGNFKEVMNWQDYVGFLTNWAVSSDWECSFKRVTEAGNVVFLEL
                     EERSRMGDFSSVVNSVSVYEFNGAGKIRHIDIYLQMELPGSEVLKNYEGVL"
     gene            complement(16787..18157)
                     /locus_tag="PLW13_00405"
     CDS             complement(16787..18157)
                     /locus_tag="PLW13_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY68856.1"
                     /translation="MTTHAVKPGNWVEHTMAWEELDFDKINMRISTDRYTSREYAGRE
                     RELIWSRVWQVAGRVDELPANGDWKEYRIYDQSYVVVRGNDGKIRGFVNACRHRGNVL
                     CRDKKGHSNGRFTCPYHLWSYGLDGRLVGVARPDLVGPIDKSELGLLEVPVDTFAGFI
                     FLNPDPAAKPLAEFLGNEVIELLAPYRLDEMTPVMDVREPLECNWKVVMDAFSEAYHI
                     VGVHPQLLAVIVPDVVKNRNAWFGDHALACAPFEVKMEKGFGPEDEVKGIMALPATFP
                     GVAEVLPIFGKLVDAFRDANGALNFPAGVSGRTLLQQATRETLTLKGLDVSGLSDDQM
                     SDNQGWFLFPNFMMTIRAGEAHVIMSVPHPDGDPNRCIWHVASYMWLPPEVREQYRAQ
                     LIDVEVPGSYQYFEALQQDYEQMPRQQLGLRNHTLKYLHLAQEEVNVARFHMVVDRYM
                     EGAATS"
     gene            18269..19204
                     /locus_tag="PLW13_00410"
     CDS             18269..19204
                     /locus_tag="PLW13_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY68857.1"
                     /translation="MDRLQTMKTFVRVVDEDGFAAAARALEVDQALVTRQVADLERHL
                     GVKLLERTTRSMRLTEAGETFLARCRDILSDVAEAEAMVSRSHQDMVGRVRLGLPTVF
                     GMEVTAQQLTRLHEEFPDITVEMAMLDRAVDPVAEGFDVVTMNATLGVSATAVARPML
                     EVPLLLCAAPRYLQRHPPPTTPQQLAAHRCVAQWASGDGDHTRERWTLEQADGTRESV
                     DVPVALRTNTYALSLEAVRCGLGVGRLTPPLVSDDLATGRLVPVLPGWHAGQLSFNLV
                     YPGRRMMPRRVRHVIDAIMAQVDEAEAASGGPGRG"
     gene            complement(19259..20515)
                     /locus_tag="PLW13_00415"
     CDS             complement(19259..20515)
                     /locus_tag="PLW13_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY68858.1"
                     /translation="MAMGMSKNAATEWRNFWLLPFVAALGYSTSVIHVYSLGPFIEPL
                     QQAFGWSRAQISIGLTIASFISAIFCIPVGILVDRIGPRRVGLIGVLLMSGAFALLGT
                     TTGTTANWVALWSIVAVGTLWVQATVWTSAVASRFEASRGLAFAITLSGAALSAAVAP
                     VLAAWLIGAYGWRTAFVAMGGIWSALVFPFLLLCFRGAHDGEASKHATTTSPVKVLKG
                     VTLSQGLRSPTLYKLLLASGLFSFTAIGAVVHFVPILTDSGATPLAAAGVAALVGIFS
                     ILGRLGTGFLLDRFPGHIVGAVAFMLPIVSALLLLFDGANPLSQAVAAAALGLTVGSE
                     VDVIAFLAAKHFGLRSFGALYGALVMALSLGTAFGPLAAGTVFDRFDSYAPFLMLAIV
                     LMGVSATALLSLGAAPETLETAAPSR"
     gene            complement(20516..21283)
                     /locus_tag="PLW13_00420"
     CDS             complement(20516..21283)
                     /locus_tag="PLW13_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY68859.1"
                     /translation="MSDSVLFDARDDGIAIITLNRPDTRNCLSREVREGLRAAWNRFE
                     RDPALRVAILTGAGEKAFCAGGDLKEMVETGMQVPPRDMFPLPYDNIELSKPTIAAVN
                     GVAFAGGWMIAQGCDLCVASTNAKFAITEVKVGRGSPWAAPLIHMIPQRIMMEIILTG
                     KPITAQRAYEIGLVNRLAQPERLMDVALELAGEILDGAPLSVRAGRETVMLATEMGRS
                     AALQAARAAHEHAYMSDDAQEGPSAFAEKRRPQWKGR"
     gene            complement(21283..22554)
                     /locus_tag="PLW13_00425"
     CDS             complement(21283..22554)
                     /locus_tag="PLW13_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQY68860.1"
                     /translation="MTVITDAAGLDLARFLRPGDRIVMGQACGEPTTLVEALIAQGRG
                     IGGLSVFIATSFSGLFTPETAASFALSSMGAIGALRGMTKAGKLAVIPAHVSQIGPMI
                     TAGIIGCDVAFIQVSPADESGNHSCGLISDHVRAAVDRARVVIAEVNEQVPHTPGETI
                     PAASIDVAVHVSRAPVEVPTGRISATDQAIARHCAAYIGDGSVIQTGVGAVPDAILRL
                     LHDRKDLGVHSGMLGDGLVELVEAGVVTNARKEVDAGVSINGALIGTKRLYAWAQRNP
                     AIRMCSTAYTHDASVLARLTRLVTINSALEVDLTGQVNAEQSGSVYMGGTGGQVDFVR
                     AGARSSGGHSIIALPATAKGNELSRITIGLSGPVTTARSEVDVIVTEFGAAELRGQTL
                     AERTRRLIAIAHPDFREELERASHTIEQRGF"
     gene            complement(22554..23441)
                     /locus_tag="PLW13_00430"
     CDS             complement(22554..23441)
                     /locus_tag="PLW13_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HQY68861.1"
                     /translation="MAIQEDIFSARSWLFAPGDSEKKMEKATAGPADIVIFDLEDAVA
                     DAEKPRARSLIADFLMAHARGRERLWVRVNPLDGPHTLADLAAIMPAHPGGIMLPKAR
                     GRADVELLDHYLSALEVANGIEQGSTRVIVLVTETAEAMFTTGSYAGAPRVVALSWGA
                     EDLADSIGASSNRNADGSYSFTYELARSLCLLGAAAAGVAAIETIQGDFRDLDGLRQR
                     AEKVRRDGYRGMLAIHPAQVEVINAAFTPGEEELAAAQEIVDLFAANPGVGTIGHKGA
                     MLDRPHLARARLLLRQAGR"
     gene            complement(23494..24660)
                     /locus_tag="PLW13_00435"
     CDS             complement(23494..24660)
                     /locus_tag="PLW13_00435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY68862.1"
                     /translation="MDLSLTDDQQNIRETVLRHCSRFADEYWLERDRDAVFPHEFHQS
                     MAEAGWLGIAMPESVGGAGLGITEAAIMMQAVAEAGGGMAAASSIHGPVFSLEPIARF
                     GTQEQQQRMIPPVLRGEHRICFAVTEPNTGLDTTRLKTRAERRDGGYLVNGEKIWITN
                     AHVADKMMLLARTTPLDQVKRKTDGLSLFFTDLDRSKIEHRLIPKMGRHAVGSNMLFI
                     NDLFIPEADRIGAEGDGFRIILHGLNPERILIGAEAIGLGRAAIQRAARYARERIVFD
                     RPIGMNQGIQHPLAKCWAQVEAANLMVMKAATLFDKGQECGVEANAGKYLAAEAGFEA
                     CHTAMLTLGGMGYAQEYHVERYLREVLIPRTAPVSPHMILNFLAEKVLELPKSY"
     gene            complement(24724..25890)
                     /locus_tag="PLW13_00440"
     CDS             complement(24724..25890)
                     /locus_tag="PLW13_00440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY68863.1"
                     /translation="MRVIDLTAMVMGPYSTQIMADMGADVIKIEAPEGDNTRNISVGP
                     EPGMSGVFVNVNRGKRSVVLDLRSEAGKSALRELVRHADVFIHSMRARAIARLGFSYA
                     DVLAINPSIVYTNCYGYGRRGPDADLTAYDDTIQAECGLPVVQQMLTGEANYVGTIIA
                     DKVAGMTALYATMMALFHRERTGEGQEVEVSMFETMASFMLVEHANGAMFDPPLGPAV
                     YPRVVAPNRRPYRTRDGHISALVYNDKQWSAFIESVRPAWASDSFATLAQRARQIDTV
                     YALLGETFRERTTREWLDLLRSLDIPAAPLRTPDELFDNPHLDAVGFFETVETPNGPV
                     RFPGVPTWFSQTPGHVAGPAPRLGAHTREVLEEFGLSAPEDSAVQREPSSGGIV"
     gene            complement(25982..27349)
                     /locus_tag="PLW13_00445"
     CDS             complement(25982..27349)
                     /locus_tag="PLW13_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY68864.1"
                     /translation="MSEKPRIDETSEDLAYPVIYPVEAYISEEYARAERDKLWRKVWQ
                     MAGRLEDIPEVGNYLSYEILDDSVLIVRTAPDQIRAYHNVCPHRGRRLVDVPQGAKNA
                     RGRRSQFICGYHGWRFNLEGANTHAPYKEDWQGALTDACAGLSKVNVDTWGGWIWINM
                     DRECEPLRDFLEPAATMLDPFQLQNMRGRWRKWGIFDCNWKVALEAFNETYHVQTTHP
                     EFMKFGEFRGWARVQGKHSNIGYDAPKGMDENQSAKLRVGKGDPRISTAEMQLYTWQK
                     ANTNTTQTLVDAALRLKDELPEGTPADQVLKHWLESARRDDEARGVVWPTVDPAHVGR
                     SGTAWQIFPNFQIGHSVNNALCYTARPCGYNPDKCIFEAVVYQLYPKGEEPQPEWEYC
                     EATAEDWCYVLSQDFSNMAAVQQGMKSLGLSGLKPNPYMERSTANLHRNLAKYMGTGA
                     PRELE"
     gene            27661..28470
                     /locus_tag="PLW13_00450"
     CDS             27661..28470
                     /locus_tag="PLW13_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQY68865.1"
                     /translation="MRSRAESGAGRADPPGAAHGATGQARVDSGAPLYLRIARALKED
                     IVSGAYMVGSLLPTEEALCERFSVSRYTVREALRLLRDDGLVSSRKGAGTVVIPPRSS
                     GSDIHQVMSINDLLAFGSDTRFAIESIRTVTIDARLASRSGLPVGEEWLEVCGYRHAE
                     GVDAPVCRAEYYINRAYAAVGRLLQRHSGPIFPLIEDMFGQSIVEVQQQISATLVSPA
                     LAAALRVKEGSAALEVRRSYKTDDGKIAQVTINTHPASRFQHAMTMRRARA"
     gene            complement(28484..29149)
                     /locus_tag="PLW13_00455"
     CDS             complement(28484..29149)
                     /locus_tag="PLW13_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQY68866.1"
                     /translation="MEFVHWFIDFFLHLDRHLAEIIAQYGVHTYTLLFAIVFCETGLV
                     VTPILPGDSLLFAAGAFAALGSLDVWVLFWLLTVAAILGDAVNYSIGRYVGPKVFHYP
                     KSRFFNPEHLQKTHAFYEKYGGKTIIIARFIPIIRTFAPFVAGIGAMSYGKFAAYNVV
                     GAVLWVGICVFAGYLFGNVPVVKENFSVVVVAIVVLSLMPAVIGVIRHRREAAAQRSP
                     AGS"
     gene            complement(29427..31034)
                     /locus_tag="PLW13_00460"
     CDS             complement(29427..31034)
                     /locus_tag="PLW13_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY68867.1"
                     /translation="MADTRAPAAATGLRRLFMRKSVAQMHAEHAQGELKKSLGALNLV
                     SLGIGCIIGTGIFVLTGRAAAQFAGPGIMISFVITGVLCAFVALCYAELAAMLPVSGS
                     AYSYSYASMGELVAWFMGLLLVLEYGLAAATVAVGWSSYAVSLLHDIGIIIPPELTAA
                     PGVAVRQGGEIVAHGVANVPAILAIGAVTALLVVGIQESATANNIIVVIKLAVVIAFI
                     CVGAFFVDPANWHPMIPEQVPPPPEGAERGLWADIRRALLDVITAENTSRYGIGGIIT
                     AAATIFFAYLGFEAVSTAGAEARDPARDMPIGIIGSLLVCTALYILTSAVLVGIVPYA
                     ELDTPAPIAVAVNAMGMPWFAVLVKIGAIAGISSVMLVLLYGQTRIFYTMARDGLLPA
                     AFARVHPRYRTPWIDTLIVGVVTAGFAGFMSLDALANLTNVGSLAAFGLVCLTVLYLR
                     VTQPALDRPFRAPLFPLVPVIGALMCGLLLMSLMAHAETRDFFLVYLGGGILLYFVYG
                     MRHSKLGKGEIVTGAEPVPDLPKRLDV"
     gene            complement(31226..33004)
                     /locus_tag="PLW13_00465"
     CDS             complement(31226..33004)
                     /locus_tag="PLW13_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQY68868.1"
                     /translation="MTSSTTDADERSIAASSCADLIVQYLALLNVPYVFGVPGGNIDP
                     LLNALARHQQASGVRWVLTRSEAGAGFMADGFARASGLVGVCSATSGPGCTNLLTAVS
                     SAYVEGVPLLVITGQSAISTFGRGAMQEGGGSGADIILMFRGCTRYNALVSHPEQLEH
                     NLLAALSHATGPTPGPVHLSIPQDIFSAPIASGRNPMSRYAFFGQEITPDRLLLNRLN
                     ILLRAYRRGVFVIGEGCAGAMAEILRYAEHRDWPIVTTPAGRGLMSADHPLYRGVFGT
                     AGHESARRTLTADEADIVLVVGANLDENATCGWDGSTILSRRLLHISSNPDHLARSYM
                     AQLNLMGSPRIVFAYLNQASAGLPDLEAQADNAALPALRNAHGLPRHISLLHVEDCGT
                     DTVPLNPRRLFWMLSQKVPPRTRLYADAGNAFFWALHYWHPRGERVVTANRMPVSMGF
                     AAMGWAIGAAVGGKLADPRDPVLCITGDGSYLMNAQEISVAQQLGLNVVYLVLNNGVL
                     GTVKHGQRMRGLQNTANELPPTDFALMARAMGVESYRIRSLDELDTLGVEKLFRRHGP
                     VLLDVLVDAEIAPPIGQRVRNLQGPA"
     gene            33252..33899
                     /locus_tag="PLW13_00470"
     CDS             33252..33899
                     /locus_tag="PLW13_00470"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY68869.1"
                     /translation="MTSTRNSSTAKHWTTFRGARALKSSHPEVRQLKRQSEDPSLHGN
                     KVWHSSFALIDYLHRHPLPRGARVIDVGCGWGLTGIWLARTFGARVLAVDADPAVEPY
                     LQLQSRINGVQVEFMAGRFEQLNARLLDGADLLIGTDICFWDEMVDPLYKLLRRAGNA
                     GVTQAIVGDPGRPPFWSLAQRVEQKLGGQVLAHASGRRGSAPKQLLITDFAKLRR"
     gene            33907..34341
                     /locus_tag="PLW13_00475"
     CDS             33907..34341
                     /locus_tag="PLW13_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HQY68870.1"
                     /translation="MSAHAAGLPRWEHYPHEADIGVRGIGATREEAFEQAALALTAVV
                     TDPAGVSPGEALAIGCEAPDDELLLLDWLDALVFAMATRGVVFARFRVTITGHRLTAT
                     AWGERVDRTRHQPAVEIKGATCTTLRVALEDGRWIAQTVVDV"
     gene            34381..35811
                     /locus_tag="PLW13_00480"
     CDS             34381..35811
                     /locus_tag="PLW13_00480"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQY68871.1"
                     /translation="MDLNLLQRRGEFAWEIPRHGAMRVPGVLYASEPLVLDMDRKVYE
                     QVVNVATLPGIVQAAYAMPDAHWGYGFPIGGVAAFDADAGGVVSAGGVGFDISCGVRC
                     LHTGLDFDTLMTQQRALADVLACKVPAGLGSTGAIRLGAREMDDMLAGGARWAVEQGW
                     GEDADLERIEEQGQMRDARPGCVSAHARERQRDEMGTLGSGNHYLEVQRVAQLFDAEA
                     AAVFGLRENDVVLSIHCGSRGLGHQIGTEFLKRMVVAAPRHGIALPDRELACAPIDSP
                     LGQEYLGAMRAAINCALANRQIITHLAREAFAQVLPRASLRLLYDVSHNTCKLEQHTV
                     DGRPRRLWVHRKGATRAYGPGHPDIPPALRSIGQPVLIGGSMGTSSYVLAGTREGEAC
                     SFSSACHGAGRAMSRHQATRQWQGRAVIDQLAARGILIRSPSARGVAEEAPGAYKDVG
                     AVVDAAEAAGLARKVARLEPLVCIKG"
     gene            36455..38596
                     /locus_tag="PLW13_00485"
     CDS             36455..38596
                     /locus_tag="PLW13_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY68872.1"
                     /translation="MQMIFAAAALVATLIGCAGAVTGQLPLDYPDTPRGDVVEHYHGT
                     RIADPYRWMEDLDAPAVADWVGAQNAVTTSYLAALPLRAHFRERLTALWDYARTGIPQ
                     LEGGRLFYSRNSGLQRQAPVYMRAGLDAEPELVLDPNLISADGSVSLAQYTPSPDAKL
                     LAYALAEGGADWRTIRLRDIASGRDLADEVRWMRFSGIAWTRDGKGFFYSRYPEPPAN
                     KVLEAPLSGHALYYHRIGTQQADDVLVYQRPDLAGWVLGGSVTEDGRYLLITLAKGAG
                     NNNRLHYADLADPLAPRLDAPVRALVESDDAEYAPIGNVGAQIYLRTDRDAPNRKIVA
                     VDTRAPDPANWRTVVPEQAHALENVALVGGRLAAQYLVDVSSRLRLFALDGTPQQEID
                     LPGVGSVFGLSGRQDARDVWYAFSSPLMPTTVFRYDPATHRSTAFEPPEAPMDASRYE
                     TTAGFATSRDGTRVPFFMTARKGLRRNGANPVMLYGYGGFSVSTLPAYRADVPAWLEL
                     GGVWVTANLRGGAEYGEAWHRAGMLGNKQRVFDDFVAVAQHLVSENITSPARLGMMGG
                     SNGGLLVAAVANQRPDLFAAALPAVGVFDMLRYDRFTGGKLWVEEYGSAGDAAQFPFL
                     LAYSPLHNLKPGTCYPATLVTTADHDDRVVPSHSFKYTAALQAAQGCARPALVRVEVA
                     GSHNYRPTDRLIAERADQWAFASEALGLHRP"
     gene            complement(38631..39818)
                     /locus_tag="PLW13_00490"
     CDS             complement(38631..39818)
                     /locus_tag="PLW13_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HQY68873.1"
                     /translation="MRELSARATLIALVAFAAVSTDLYLSGIPGIVADLGVGHAEGQL
                     TLSLFMVGFAAGQLLFGPLSDYHGRKPVVAAGLWLYALATLLCALAPSITTLLGARLL
                     QGLAAASGPVIARAIVRDRYRAEDAARMMALLAAAMAVVPLFAPVLGSWLLYCFDWRA
                     QFAGMLLFGLATLAGLRALVESCPSIGIGPLDLWRVFGQFGTCLRSRAFVGYQLCGGA
                     AFASSFAYISSASFIMIEMLGVAPQHFGYTFMLCVAGYMGGALLSSVLVTRIGIRRTL
                     GRGVAVNLAGIGLLCALAFGPAQPLLAVILAIFLCFLGSGLCLSNSQMGAISQFPMAA
                     GGASAVFGFVQTAMAATSGYVVGQSYDGTLVPTALAMTCAVLLSALGYLVLRPVRGEA
                     ARA"
     gene            complement(39872..40294)
                     /locus_tag="PLW13_00495"
     CDS             complement(39872..40294)
                     /locus_tag="PLW13_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQY68874.1"
                     /translation="MIRKCAACGAANRVPAARLCDTGRCGACKAALPPPAQAFEADAA
                     MFDEIIGGAVVPVLVDFWAPWCGPCRMAAPEVAKAAANLAGRALVLKVNTEEQPALAQ
                     RYGVRSIPNFAVLRDGVLKRQQPGLMGHRQLEALVLES"
     gene            complement(40291..40851)
                     /locus_tag="PLW13_00500"
     CDS             complement(40291..40851)
                     /locus_tag="PLW13_00500"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HQY68875.1"
                     /translation="MVLFTILGVAVLGRAAAAEDARLAASRDIASGMQAALKGELAQA
                     MATAGPVGALAVCRTRAPAIAAQAGNAAGVRVSRTALRLRNPANAPDEQGRAVLEQFS
                     ERLGAGDAPGQLEYFAPTPDGGARFMAAIVTQPPCLVCHGATIAEPVRQALREQYPAD
                     AATGFAVGELRGAVVVDWPAGAQGAR"
     gene            40982..41356
                     /locus_tag="PLW13_00505"
     CDS             40982..41356
                     /locus_tag="PLW13_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY68876.1"
                     /translation="MTATAVRDQFQTLVADTREMGRHAESAAAMLKCLANPHRLMILC
                     TLCEGELSVGALNARIPLSQSALSQHLAVLRSERLVDTRREAQTIYYSVRPGPALDVI
                     RVLHEHFCAESTEPPDAGTPHS"
     gene            complement(41399..41815)
                     /locus_tag="PLW13_00510"
     CDS             complement(41399..41815)
                     /locus_tag="PLW13_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HQY68877.1"
                     /translation="MKAMKAGARASAELVASEADMASALALSVEDEFPEVFATSRMVA
                     LMELAAARCMKPLLAPGQLSVGVNVNVTHLAATPKGVRVRAEATFTGMQGKLYGFELQ
                     AWDAGGLIGQGSHTRAIIASERLLTGARTRNESSAS"
     gene            complement(41860..42396)
                     /locus_tag="PLW13_00515"
     CDS             complement(41860..42396)
                     /locus_tag="PLW13_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY68878.1"
                     /translation="MGKGNPRAGTPATVSGNVIRDSSMGLATQLIDPIVRLNDSVWRR
                     NSVRLALVEMLRLRNARAVNCVFCKAARYDVARQDGLTEDKVAQIDDGFADSGLSELE
                     KLVLGFADVYLRDPGHFDPQLIARLREQFSEEQICHMAVALATFNASSKCAVSLGGMP
                     EFVPVMEVTLPPPVEVSA"
     gene            complement(42407..42961)
                     /locus_tag="PLW13_00520"
     CDS             complement(42407..42961)
                     /locus_tag="PLW13_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68879.1"
                     /translation="MGALPLHDANGWRATVRQPPRRHTEEELQMQADGAVTSETLSVA
                     LAPGGAMARDFNALWEQLWRQDHVPARLLELCRLRLARLHGASAEAAVLRGLERDPGR
                     IEAVLSGRYHGSAYFDAAEQAVLEFAEIYAQDPGALTDELADGVKRHFGEAGLVCLIE
                     ALGFIEGRIRLALMFTALGAATAH"
     gene            43087..43998
                     /locus_tag="PLW13_00525"
     CDS             43087..43998
                     /locus_tag="PLW13_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQY68880.1"
                     /translation="MRWKDERRSENVEDRRGQSASPRRASGAPVLLQLLPMLLRSRGG
                     RYLLGILAVVVIGAQFLGVDILSLLTTGGPAVQAPSGQRAAPSAAEQERAEFVAHVLG
                     STEDTWRQQFAGLGRTYEDPRLVMFSASVQSACGYAQAAMGPFYCPADHKVYLDLSFF
                     DELHQRFGAPGDFAQAYVIAHEVGHHVQTLTGISAQVQAAGRGASKAQVNALSVRQEL
                     QADCFAGVWAHHAESSRQILDPGDLEEALGAATAIGDDRLQKQAQGYAVPDSFTHGSS
                     EQRVRWFRRGFESGDIGSCDTFAAQNP"
     gene            44143..45306
                     /locus_tag="PLW13_00530"
     CDS             44143..45306
                     /locus_tag="PLW13_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQY68881.1"
                     /translation="MDPMLYMAAAVGAAAALALAATAILTRGVKPQAGAANPGSAPPE
                     RRRAVATDTGAGIAPALAMVATEEPAPLPDALNGFWLADEHEITTQRSTQLATQLRHV
                     AMPPRELQHFMATDIMAGNAAQELSELVMSEPRLAAKVLGRANSPFYGLQSPIASVAH
                     AITYLGMNTVRNMALSFVLQESFAVEDAALGRYCDSVWEAGRIASELCSLLAPRLGFP
                     DTGTLGTQTILSFLGDLAVPSLLGDEAGAIQELDFVERLRFEQEQLGASSVLLGTIVI
                     RAWGLPAPIVDAVAAAGRVVVTPVEQADPAQAPRSALIYSCARIGEAIARQRIAGPDE
                     IELRNAAAPELHHLQGYLRLPQLARLPELLHAPDVRLALARTIAAVRNAAAPG"
     gene            complement(45348..45905)
                     /locus_tag="PLW13_00535"
     CDS             complement(45348..45905)
                     /locus_tag="PLW13_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY68882.1"
                     /translation="MRREQVLTAAAACFQRHGFHGASMAQISREAGMSVGHIYHYFAN
                     KEAIIAAIVAGDQQQILAIDDEIRSHEDWARAMVEHVPHGLERAVEGPRGPLLLEVLA
                     EAARNPAVAALVRQADEEVRRKVGETLRIASGHGDRLSDPEGRCEVLSALYEGLQIRG
                     VRHPGMDRAATARVMQRVMRALLEP"
     gene            46077..47243
                     /locus_tag="PLW13_00540"
     CDS             46077..47243
                     /locus_tag="PLW13_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY68883.1"
                     /translation="MIQSLQRATMLTALVLLAACGNDGAGTPAAPPPPEVGVLTIEPR
                     ELQLNVELPGRTSAYLVAEVRPQVSGIVRERRFTEGAEVVRGAPLYVIDPDTYQAEVA
                     TAEAALAKAEAAANTARLRAERFRALVARHAVSQQDADDANAAEQQAVAEVAQQRAQL
                     ARARIDLQRTRITAPISGRIGKSDVTPGALVTADQPQALATVQQLDPMYVDITRSSQD
                     LLRLRDDMQAGRLQGAQSDAAVVTLVLEDGSTYPHPGRLAFSDVTIDPTTASVTLRAV
                     FPNPEQRLLPGMYVRAMLAEGVRAEAILLPQQALIRDAQGNASALVLDENATATLRPL
                     QVGRSVDGNWLVESGLRRGEVVVLDGLQKVQPGTRVVPVARTVAPMAVAARTTH"
     gene            47243..50362
                     /locus_tag="PLW13_00545"
     CDS             47243..50362
                     /locus_tag="PLW13_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY68884.1"
                     /translation="MAAFFIERPIFAWVIAIVVMLAGALSIRTLPVSQYPEIAPPSVS
                     VSASYPGASARTLEDTVTQVIEQNMKALDGLLYMSSTSDSAGNASVMLSFQPGTDIDI
                     AQVQVQNKLQLATPMLPREVQQQGLRVNKAMSNLLLVVAFYSEDGSMAELEIADYVVS
                     NVLDSISRVKGVGDIRVLGGAQAAMRIWLDPARLHSFALTPADVRAAIEAQNAQVSAG
                     ELGGTPAIPGQQISATISAQSRLQTPEQFGAILLRVNTDGSTLRLRDVARIELGSETY
                     SVRSRFNGKPAAGLGIMQQSGANALETAAAVRARLAELEAHFPPGLHLAVPHDTTPVV
                     KESIRGVVITLLEAIGLVFLVMLLFLQNLRATLIPTLAVPVVLLGTFAVLAAFGFGIN
                     TLTMFAMVLAIGLLVDDAIVVVENVERVMREEGLPAREATRKAMGQISGALVGIALVL
                     SAVFVPMAFFGGSTGLIYRQFSITIVSAMVLSVLVALIFTPALCATLLRPHAGGESAH
                     GLFGWFNRVFAAATGRYTDTVAAWLRRGLRYLVLYAVLLAALALLFMRLPTAFLPEED
                     SGFIVTMAQLPPGATLERTQKTMEHISRHYLENESALVTKVFATSGFSFAGSGQNAGQ
                     AFVELSPWEERTDPSQGVQTLAERARQALRSLPDGIAFPMVPPPVRELGNATGFTLFL
                     QDRGGLGHAALMDARNRLLGMAEQHPQLRGVRPNGLEDTPQLQLDIDHDKASALGLSV
                     AGINDVLSTAWGGRYVNDFIDRGRVKKVYVQGNAEARMQPEDLEKWYARNDKGQMVPF
                     SAFASAHWTYGSPRLTRYNGIPAVEIQGQAAPGVSTGQAMQAIAGIIAELPPGIGFEW
                     TGVSLQEQQSGGQAPLLYALSLLVVFLCLAALYESWSIPFAIMLVVPLGVFGAVLAAT
                     LAGKANDVYFQVALLTTIGLSAKNAILIVEFAKTRQEHGAGLVEATLEACRLRLRPII
                     MTSLAFVCGVLPLATADGAGSGAQNAIGTGVVGGMISATVLAIFFVPLFYVRVRRRFA
                     AERSPTGTD"
     gene            50387..>50710
                     /locus_tag="PLW13_00550"
     CDS             50387..>50710
                     /locus_tag="PLW13_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQY68885.1"
                     /translation="MTTEIASREPGWAELLRGHLREHSLAALGLGCALVAVLALLAQS
                     IAQVLGGEAAPGLRLALLGGAAGFATTTLGALPALALRGLPQRIEDSLMGLAAGMMLA
                     ASAFSL"
BASE COUNT         8791 a        17089 c        16179 g         8651 t
ORIGIN      
        1 cggcagattg ccgcgaaaac cgcgcgattt caggcccagt tgcaccattt ccatgttcga
       61 gaagtcctgc gctatgacct tgcgccagtg ctcctcggtc gcgtccgcat aagtccactc
      121 ggtcttcggc tcctgcccct gcggatagcg ctcgatggca taggcttcgt aaatgcactt
      181 gtcggggtcg gtgccgtaag gacgcgtccg gtaacacagc gcgaaggtgg gcccctgcaa
      241 tatgcccatg ttggggaata catgccaggc caggcccgct gtcgccatct gctcgtcact
      301 gatctgcggc cactccacgc ccatcgccgc gtagtccgcc ttgcaggagg ccagccaatg
      361 acggtgcact tcgtcagcgg gggtaccctc gggcaattcg tcgaccaacc gtagcgctgc
      421 atcgaccagg acctgcgtgg tgctggcgcc gaccgtttcc cagaactccc gctgcatttc
      481 tcccatcatg acccgggcgt cggcaccctt gccggtacga tgcaccgttg tcgtggcggc
      541 ggaaccctca tcctggtgat cgggcgcgtc atagccgtcg ttgccgtgca atccctgcac
      601 tttgctccag gcgtagaagt cgccgtattt cgtgagctgc ggatgggtag ccgccacatg
      661 gtagggctcc atgaaggctt ccagcgccac cttccagttg caatcgaaga cggtccattg
      721 gcggaaccgg tagcgcatat cctcgaacct gaacggatcg agaataccgg ccgcgggctc
      781 gagatattct cgcagggaga tgcaattggg atccatgttg acccagatcc agccgcccca
      841 ggtgtcgacc ttcactgcgg caaggcatgt gcgctcattc gtcagcgtgc ccttccagtc
      901 gtccttgttc aggatatggg tattgctgcc ctgaaggtcg aaggaccagc catggaagcc
      961 gcagaagaat gactgcttcg tcgcattgcc gcgcgcacca ttgccgccct ttggcgtcga
     1021 gaccagccgg cgtccgcgat gcggacagac attgtgatag gccctgatcg tgccggttgc
     1081 ggcgcgaacg atcaggatcg aatcattggc gatctcgtag gtgatgtagt cgccgacctc
     1141 gggaatgtcc tcgacacgcc ccgcatgctg ccagaccctg gcccacagct tcccgccttc
     1201 agcttctgca tattccctgg aaacataggc gtccacggga aacgtcagcg gctccgacaa
     1261 atcctcgagg atgatatcgg ccttcgtgtt catgccagcc tcctgtttcc ggccttaccg
     1321 ggagagaacc agaccgtcga gatcgcccct ggaacgccag ttttccagaa cccgcatgaa
     1381 ggcgtcccaa cccgggccgt agagttcacc caggtagaag cggtatcgct cgttgccgtt
     1441 ctcatccaca tcgggcttgc cttcgttatt gaagtagctc ggggtacaac tggcaatcca
     1501 ctccgccttg ttgacctctg tttcacgcat gtgcctgatc catgcgtcct gggcttcctt
     1561 gctgacttcg gcagcaaggg cgccgcgatc gagcacttcc ttgatgatgt agagcatgtg
     1621 cttgacctgc tgctcgaagt tgtaggtgag cgtcgagttg aggccgccct ggtaatagcc
     1681 cgtcgtgaac aggttgggga aaccatgggt catcatgccg tgcagcgtct tgtagctgcc
     1741 gttccagttg tcgtacatgg acaatccgcc acgcccctcg aagaccggta ttccccagcg
     1801 acgatcgagg tcgctggtca cctcgtagcc gctggcaaag agcatgcagt ccacctcgta
     1861 ctccacgccg ttggcaacga agccgttttg cgtcatgcgc tggacaccct gcgtctccga
     1921 cacatcgatc agcttcacat tgtgacggtt gaaggtctgg tagtactcgt cattggacgt
     1981 gggccgcttg cactggtagc ggtaccacgg cttgaggagt tccgcggttt ccttgtcctc
     2041 gacgatcgcg tcgacccggc gacgcaggcg ctccatcaca cgaaagtcca tttcctcgaa
     2101 gcgcgcggcg aactcctgcg ccgtgagctc tggccagccc tgctcctcct gttcggccga
     2161 cagattgcgg ttaacttcag tccagatatc gcagatcagg tccttctcgc ccctcaggaa
     2221 ccgctcgttg gcgccgcggt gaaaattgtc gcggcgctgc gcctgccagc cgggcttcag
     2281 cgtcttgacc cattcgggat cggtttccga gtttctgcgc gagtccacac aggagggcgt
     2341 gcgctggagc acatagaact gcttcgcata cttgccgagg taaggcacca gctggatggc
     2401 cgttgcaccg gtaccgacaa gggcgacacg cttgtctgcc agcttgtcca gaactggtcg
     2461 tttttcgcag ccgcccgtgt aatcgtagtc ccagcgcgcg gtgtggaaca tatgtccctt
     2521 gaagccgtga atgccgggga tgccgggcag cttgggtatg ttcaacagcc cgttggccat
     2581 gatcacgaag cgggcgcgaa tgtcgtctcc ctggcgtgta gcgatacgcc agcgcttgag
     2641 ttcctcgtcc caacgcagcg actccaccat ggtgtggaac agcgcgtttt cgtagagctc
     2701 gtacttcttc gcgatgctct gggcgtattc gcggatttcg tggccgtcgg taaacttctt
     2761 cgacggcatg aaacccgttt cttcgagcag cggcagatag cagtaggcat cgttgtcgca
     2821 ctgcagaccg ggataacggt tccagtacca cacaccgccg aagtcacccg catgatcaat
     2881 gtggcggaaa ctggtgagac cggatttcct gatatgaacg cccgccgcga tgccggacca
     2941 cccgccgcct aggacggcga tatcgatttc ctcgcagacc gctttacggg gaatgaccgg
     3001 cgtgtaggga tccgcctcat aggccgccgc gaagtcgtcc accggacgga catactgctc
     3061 ctgcccttcc ttgcgaatgc gccggtcgcg ctcctggcgg tacttttcct tcaatgcagg
     3121 gatatcgatc tcttcagccg agggcaaacc cggcgacttg gtgtttatca tttccagact
     3181 cccggggccc cggcccttga cgctgacgac tcaggatcaa aatttataat caacagcatt
     3241 gttgactagt aaacatcgat tgcctgcacg aatcaagtac cgcgtcgaat atgcttgcgg
     3301 attccggcca attgctcaga cgatcggctc ctccccctcc gtcttggtgc gatgcagcag
     3361 ccgtccgcaa tcggggtcat acggcatggc gcggtgcatc gtgccggtgt tgtcccacat
     3421 gaccgcatcg ccaaccgacc attcgtggct gtaaacgaac cgcggctgcg tcgcccaatc
     3481 gcgaagacgg accagcaacc tcgtgctctg cgtcgggctc atgccgatca cgtgatgagc
     3541 ggtacagccc agcaccagcg atttgcgccc cgagcggtgc ttccacacca gcggcaactc
     3601 gacctcgccg atcctgatca tctcctcgag cttggcgtag ctcggctccg gctcgtagta
     3661 gaacagcgag gtccatgccg agtgcatcgc tctgagccct tcgatctgct ttttttcctc
     3721 gtccggcaac agctcatacg cggcataggt attgcaaaac tcggtgttgc cgccccatgt
     3781 gggcagcacc ttgctggtga ggatggcaac accgatcggc accttgttca tcgtgccgtc
     3841 agcatgccag tacagcgaac ccttgagata ttcggacttc tggttgagcg acttgtccag
     3901 cgtgatgttg tagacctcct gcccctccaa ctccggcaga aaccgcccca tcgtcctggt
     3961 gaacgcaacc tgctcttcat cgttgaagtt gatctgcgga aagacgacaa ccccccgacg
     4021 ctcgagcagc tcgcgcaggt cggccgcgta ttcaccgctc agcaaggccg ccttgtcgag
     4081 gtgcaccttg ctgccgatca ggggcgagag gtccgtggca tggagcgctt cgagagtggc
     4141 ggtggcagtg gtcatgattg gtttcctccg ggatatcgac ctacgaactg acagacgacg
     4201 ggtgatccat gcagttgcac gggcgataaa caccgcgtca ctgaacaggg atcaccgcgc
     4261 gcacgcacct ggaaaatcgg tgcttactta ttcaacatcg ttgattaata ccatgggaca
     4321 agccacgtcg cccggcattg atccagatca acgaccgcgc gtcgttcaac tgcctaggat
     4381 gccggcgagt tgcccggaaa caagcgtcca cacacgcgga tcacccagtg ttcctccaac
     4441 aggaaaatgc catgagcgcc gcttctcccg ccacgattgc cacggatcca gcacgttcac
     4501 cggcagcagg caccgacggg ccctcacact ggcagacgtt gtcgcaaggc gtcggcgtcg
     4561 gccgctacac cgacccggaa ttcacccggc tcgagttcga gaagctgtgg agccgcgtct
     4621 ggcaggtcgc agcgcgaatc gacgagatcc cggaaaaggg cgactacacg acctacaaga
     4681 tcggcgacca gtcgatcctg ctggtccggg tggacgagtc cacgatcaag gcgtactaca
     4741 acttctgccc gcaccgcggc accacgctcg gccacggctg cggacacttc gacggtggca
     4801 agatcatctg cccctaccac ggctggcgct gggatctcga gggcaacatc aagctcgtgc
     4861 tcgagcgcca tgagttctgc gatggcaagc tgaaggatag cgatgtcgcg atgcgcgaga
     4921 ttcactcccg ggtattcgcg ggcttcgtgt tcatcaacct cgacccgaac ccggaatcgt
     4981 tcgacgattt catcgcgccg ttgcgccagt tcctgggcga tatcgtgctc ggcgacatgc
     5041 gccacatctg gtggaagcgc accgtcattc cgtgcaactg gaaagtcgcc caggaagcct
     5101 tctacgaggc ctatcacgtt tccgccaccc atccgcaact ggaaaaggtc ggacgcgagg
     5161 tggtctacgg tgaccgtccc gacggcccca tgatgtaccg ggacctgacc tacgatgcgt
     5221 ttgcgcacgg acacggccag ttctacggcg gcgacaagac accgatggcg ggccatgtgc
     5281 aggagccgaa atcgaccgac gatctgctcg aggagatggc ggcgcgcatg aacctgacgg
     5341 ccgagggact cggcgcgatg atactcaagg aggacatcga gctgctgctg acgctacgcg
     5401 gcaagcactt gcccccggga acgaatctcg gcgcggaata cgtgcgcctg cagtacgaaa
     5461 cagcggcgaa atcgaacagg ccgatgccca agctgacacc cgagattatc gggcgctggg
     5521 gcggcgtgac cgttgccttc ccgaacgtgc tggtcctgat gcaagccggc agcgcggcgc
     5581 tctaccgcgt attgccgcac gcgaccgatc ccaaccagtg cacgttcgag attcgctcgg
     5641 tcaagaccta ccctgcgggt gtgaaaacac cgcgcgccat cgtcgaagac atctgcgatc
     5701 ccgagcaggt cgggctcatt ccgcggcagg atctcggcaa tctgccgcgg gtacaggaag
     5761 gtctgcgctc caggggcatg aagcaggcgt ggctggcctc gaaccaggaa aagctgatcc
     5821 tgaacatgca tcgggaactc gaccgctacc tgggatcctg aaatcctgcg gcggggtgga
     5881 cgcccggcta ctcgcggctg cctgcgacag ggttgagacc atgaaccatg ccgaactgat
     5941 ggcgcgggag agcatccgcc acaccatggc cagctatacc atggcggggg atcggttgcg
     6001 ggcggaggat ttcatcgccg ttttcaccga ggatgccatc ctggaaacgg acggtgtgcc
     6061 cgcgcaggac gcctttcgtc acgttggccg gcaggcgatt cacgactgga tcgagcgctg
     6121 gcgcacacca gcggcggacg atcaaaagcc ggtacaccag gcgagctttg tgcggcacca
     6181 tctcgcgact agcctgatcg agcttgtcga cgccgagaca gccaacgccc gcacctactg
     6241 gactgcctat acggatatcg gccctgatca ctgcgggtgc tacctggatc tgttcaggaa
     6301 atccggtgag cgctggctca tcgctcatcg aaggatacgg ctggactggc gctcacccga
     6361 cagcttgatg ttcacggcca tagcaagaac aagctgacgc caggaagccc ggacgagacg
     6421 ttcgaagcgc acgcgcgaca tggcgtcatc acgccacgat ccagtggcaa gtcattccgc
     6481 ggtggaggtg tttgtgctcg agggtgaaaa gatcctgatc acgggagcca ccgggaagat
     6541 cgcgttcccc atcgcccggg ccctggcgag gaacaacgaa gtgtgggggg tggctcgcct
     6601 gcagaacgcc ggagaccgcg acaagctcgc cagcacaggg gtcgaaccgg tagcgctcga
     6661 tctgtccgct ggcgactttg cgtcgctacc cgacgatttc agctatgtgt tccacgccgc
     6721 cgtcgatccc gggcagggcg actggtgccg gtgcgtcagg accaatgcgc actactccgg
     6781 cgacctgctt taccactgcc gtagcgcgaa gggtttcgtg ttctgctcaa ccggatcgat
     6841 ctacggctac caggggcagc ggccgctcac cgaatccgac ccgcccgggg ttccgctgcg
     6901 cgccaactac agtttctcga aggtcgcggc cgaatccgtg tgcacctgga ttgctacgca
     6961 cttcgggatt ccgctgacga tcatccgtat ctgctccacg tatggcccgc tgggtggcac
     7021 gccggtcgac cggctcgagg cgatcctgca gcgcaggccc atccgcctcc atcccgacaa
     7081 gccgaacaac tacaacccga tctacgagga cgactacgtc gaactgggca ttcgtgccat
     7141 ggaagtggcc gcgacaccgc cgctcgtcgt gaactgggcg ggcagcgaga ccgtcagcat
     7201 cgaggactac tgcagctata tgggcgagct ggtcggcatc gcaccggtgt tcgagtacac
     7261 gccagcggca cacacgccgc tgtggccgga cgtgacccgc atgcacgagg tgctgggccg
     7321 gaccagggtc cactggcgcg atggcatgcg ccgcatgatc aaggctctcc acccggagct
     7381 cgtgttgaag ggggagcagt gagatgaacg atcaccgcga cagttcttct gccgggttca
     7441 tcggcacgcc atcggacgcc gtgccgcaga tcctcgccga gggacgcggt ggcatcacga
     7501 ccctcttcgt gtcgatggcg caacgccatc ccgacggggc cgatgcgcac tacctgcgat
     7561 ggcacaccct ggatcaccgg ccggagcaac accgcctcgc cagcgtgcgc gcgtccctgc
     7621 gcgttgtgtc gacgccggcg tgccgcgcgg cgcgggcggc gagcgacgag cgcttcgagg
     7681 cgatcgacca tctcatgacc tatttcttcg ccgatatcgg cgggctcgac gaattcaaca
     7741 agctctccag ggcactctcg agcgccggcc gggttccgtt cgtgctccca ccggtgcagc
     7801 gcggcgtcta cgccgtggag acccggatcg cggcgccgcg agcgaaggta ggctcggacg
     7861 tgctgccgtg gtgcccggcg aagggggtat acctgctcat cgaagagggt gcggcatctg
     7921 cgactcgatt gacggatgtg ccaggcgtcg cgggcctgtg gtctgccagt tccgttccaa
     7981 ctgacttctc cactgccggg ggcgggcagc agattactta ctgcttcctc gacgacgacc
     8041 ccgtggcaac cgccgggagg ctgcgcaggg tgctggagca gcgatgggcg gtggcgggaa
     8101 ttcagcccct gctcgccgcg ccgttctatc ccgtagtccc ctacgagtgg gatcggtatc
     8161 tgccatgact gtttaaaaca ggcggcggca gtgcattcct gcgcgccttc atgacggatg
     8221 cgcgaccaat ccggtagcgc ctagcctgca gactgcacca gctcgatgcg cgctccgtcg
     8281 gggtcggtgc aaaagaccat tggcccgaac ggcgaatcga ttgccgtctc gcgatggaca
     8341 tgccctccga atcgcacgac gcgctcgacc gcggcgttga cgtcatcgac aaccagggtc
     8401 atgtgggtaa atcccaactg gttcatcgcg cgacgctggg cagagccggt cacgccggcg
     8461 ccggggtaac cgatcagttc tatggtgacc gcgccgcatt tcacataatg cacgcgacac
     8521 ctgacccccg gcagttccat caggctgtcg tagggcgggc ctatttcatc aatcgaacgc
     8581 tcgagcacaa agcccagcgc ctcgatgtag aagcgcaggg aatgctcgac atcggatgta
     8641 caaataccga tatgcgagat ggatacggtc ggcaaaccgc tcattccgct ctcctaaagc
     8701 ccctccgccg cgaccagtgg aaacagcaac ttgtcggcca gtatggtgca gaaatactcg
     8761 ttcacctgct tgatcttgcc ctcgcgcaac aagaacacca tgcaatattg attgcaatag
     8821 gactcaccgt tgcagagcgt ggcgttgcca tcgaactcca ccaccacccg ctcgccttcg
     8881 gctgtcacgc tgtggatatc cgggtgcata ccgtcgggca ccagcttgcg gaacgccgcg
     8941 atcgtggcga gtatggtgtc gtgtttgcgc acgcccgcga atttcccgaa acccttggcc
     9001 agcgtgaatg cgtccggcgc caggcatgga gcggccgcca ccgcatcgcc cttgcccatg
     9061 gcatcgatga atgtgagcac gacctgcttg ttctgctcgc tcatgatgat ctctcctcct
     9121 tgttgtgtga atgtacatcg cgtcagcccc gcacgcgcga agagcgctcc aacgcgctga
     9181 cagcgcctat ttgcgggacc agccaaggtt cgagccgcca tcgaccggca gaaccattcc
     9241 ggtcacctgc gccgacagat cgctcgccag gtacaacgcg gcgttcgcga catcccgcgg
     9301 cgtcccctgg cgccgtagcg gctgaacccg gcgggtcatt tccgacaggt cataaaatgt
     9361 ctgggctgcg ggaatatggc ccggcgcgat gcaattgacc cggatgccgt actctccgag
     9421 gtctgtcgcg atcgacttcg tgacgtgaat gacgcctgcc ttggcggcgc gatagctgat
     9481 caggggaagc cccggcttga gtccggcgat cgaggtggta ttgatgatcg agccgccgcc
     9541 gtgcgcggcc atataccggc cggcgcactg gcagcctgcc atcacgccga acaggttgac
     9601 ggcgatgacc cgctcgaaat ccgccagatc gtcttccagg aagcgattgt ggaccgcacc
     9661 gctgataccc gcattgttga acatcacatg caggccgcca aaacgctcga cggcaaaatc
     9721 caccagttcc tgtacctgcc tggcatcggc gacatccgtt cgcttgaatg ccgcgggcgc
     9781 gccgagcatg gccgcgagcg cttcgccttg ctcggcgttc acgtccgcga tcaccaccct
     9841 ggcgccctgt tccacgaaca gctccacggt ggcgcgacca aggccgctcg caccgcccgt
     9901 gatgatggca acctggccgg caagcaactg gctcatcgag tactcatgga attgatggat
     9961 tgatcctcgc cattgacccg cgacggcgcg agcccgatca aagcaggggt tcctcgcctg
    10021 ccaatatcgt gcggtgcatc aagcggccgc tgtccagagg atacggcagc gcgcgatgca
    10081 tggtgccggt attgtcccag atcagaagat ctcccacctg ccactcgtgg cgatagacaa
    10141 accggggctg cgtcgcccag tcacgcaggc gggccagcag cgccctgctc tcctcgacgg
    10201 acatgccttc cacataatcc gcggttgcgc ccagcagcag cgacttgcgc ccggattggt
    10261 gcgtccatac gagggggcag gacttggagg gcgcgcgttg ccacatctcc agttccgcgt
    10321 agctcatttc gggattcacg tagaactgcg atcttgcagc actgtgcacg acccgcagct
    10381 tctcgaggct tttcttttca tcgtccggca gatcatcgta cgccgcgtag gtgttgcaga
    10441 attccgtctg cccacccgtt gccgagagct ttatcgcacg cagcagcgcc gcgaggttcg
    10501 ggtacggctg cagcgagcca tcgaagtgcc agaacagcga ccctttcagg taatcggcgc
    10561 gctgattgac cgccttgtcc agggatatct tgtagattcc gccctcgccc tcgctcgcca
    10621 cggtttttcc gagcgtctcg gcgatggcca cctgctgcgc atcgtctata tcgagaccgc
    10681 ggaaaaatac gacaccgcgc tgctccagga tattgcgtat gatggcggcc tcggaaccgc
    10741 tcatcaaggt ctcgacatcc gtcctgatct cgctgcccac ccgcggcgtg agatcgataa
    10801 cctgcagcgt catgatcgtt ccccctgtcc catcaactca aacgcgtcag gatcgtgcgt
    10861 gccgaatgct ttacccgagg ctgcttccac aactcgacgg ccagcgaaac ggtgatcaat
    10921 gaatagagca ggttgcgcag gttggcggca tccggcggca gtggctgctg gtagtcgaac
    10981 tggtcgaggt aggcaaccgc tgcctcggca tggggaaaaa tctcgtcgta gaatgcccgg
    11041 atctgctcca tggagctatt gacgcgtttc acgtagcgcg catgcccgtc ttcaatggcc
    11101 cactcggcaa ccaggtgttc gagttgcgca aattttgccg gcagcagcgt gctcatttgc
    11161 cgcccccctt tttctcctca cctgcattga cctggtcgac gacgaccttg tgcagatggc
    11221 gcaccagcaa ttcctcgtcg ttgaaatgaa actccctgat cgcgccgctg ttcagcatgg
    11281 cctgcatgcc ttcaagaggg ctcgagtcct cgagaatgat gtcattgagg aacgtcacgg
    11341 tcagttcctg gcccaaacgc tccttgtgcg tcgtcggcgg ccgatagaac atttccacct
    11401 cgatgatgtg cgaatgcggc cccgtcggcc aatggacatg ggtggtgaaa cccgacgcac
    11461 cgaagatcag cacgaaattg gggaagaact ggaacgagtc gaaaccgtag ttctccgagc
    11521 gggtgggatt cagcccgcga ggcaatgaac cgacgtcgat cttcttgttc caagggccgg
    11581 ccgcgcttgc ctcgaacacg cattcgatcg gcttcgagta cggggtcttt cgcgagggct
    11641 ccccggcaaa ggaaaacatc cggtgcaggc cctcgagacg gtaagccaac gcatcggtga
    11701 acggattcgg ggcttccagc gcggccttgg ccgccgcagt caggttgcca aaggaagcag
    11761 cgtgcaggta gggcccgtga tagctctccg cgaacccgtc catgaatatc ttccagttgc
    11821 actggatctc ggccttgaac cggtagacct ggtgcggacc tgcaaaggga tagccttcga
    11881 tcccgtgggc cagctcgccg aggaagtcgc gtacagactc ggtattgtcc gggttcaggt
    11941 tgatgaagat gaagccttcc cagacgtcgc actgcacggg gggcacgcga cacttgtcgg
    12001 ggtcgaaatc cagcagcaaa tccttgcgtg taggtgcgat cagggaaccg tcgagctcga
    12061 agctccagcc gtgaaacctg cagtagagtg cgggggactg gccctcgacc tcctcgaacg
    12121 ggtcgtcgcg ccagaccagc ttgttgcccc ggtgcgggca gatattgtgg aaggcgcgga
    12181 tcacgtcgtc cttgccccgc accacgatga tcgaggtact ggcgaaaccc agctcgcggg
    12241 tgaaatagct gcctttcctg ggcagccgct cgacgcgccc gacgtacagc cagttcttcc
    12301 ggaatacgtg ctcgcgctcc ttctcgtaga actccctgga aacgcagtcg tccagcgaaa
    12361 ccgggccccg gcccagctca ggataggcgt cgctccagtg cccactcggc ggcttggtga
    12421 cgatggggtc cctttccata gcgctttcct cgttatattc gccgcggtac cgaccaccgg
    12481 gtctgcggtc acggttccgt cccgatcatg catgcgtcaa cgtatcactt gtgcactgtt
    12541 gcaaaataga ctcatatttt ggaaagtgtg ttgcaaatat ggaacatcat ccctcatggg
    12601 tcacaacctg ccgtttgata tcgatgcgct gctcgtgttc gcgaaggtgg tcgagtgccg
    12661 cagcctctcg aaggcagcat cgttgctggg gatgcccaaa tccacagcca gccgcaagat
    12721 cagcaagctc gaatccgacc tgggcatcag gctgctgcgc aagaacacgc accagatcac
    12781 tgttacggac ctcggtgaga agatctacgg ccatgccctg actatcctgg ctgaagccaa
    12841 cgatgtccgc gctctggtgg aaggcagcca gcaggaaccg caaggggtgc tgcgcgccgc
    12901 cataccggtg ttcgtcggca tcgatttcgc ctcgcgggtc ggggccacgt tcctgcagcg
    12961 ctatccgaag tcccggctcg agattcgcct ggtggacaac atggtccatc ccgtcaagga
    13021 cgggtttgac gtggttttcg gagcgggccc gctgcaagac tccacgctca ttgccaggaa
    13081 acttttcacc cttgaatgct ttctgtgcgc ggcacccgat ttcatcagga ccttgccgga
    13141 accactcacg acccccacgc aactgaacac gcttccgttc attgattcgg gcttttacga
    13201 ggagccgcgc aagctgcacc tggccagagg caaaaagcac taccagttgt cgccgctggt
    13261 ccgggcgcgc gcgaacaatt tccagatcag caaacaatat atcctgcagg gactggggat
    13321 tggcgcgatg ccgaagcaga tcatatgctc gggcgaattg caggaaggaa gcctggtacc
    13381 ggtattgccg gactggagcc tggaaacggt cgatgtctac atgatccacc cgttccagtt
    13441 gtctttctcg aatctgatca gcgcctttta tgaaaccgct gtcgagatca tcacgcaaaa
    13501 caccgagagg agcgggacgc tcgatacccc gccagccccg cattgagcgg gcggggcgcg
    13561 ggacgggaac acgcgggcac ccgtccttca caccgatcga gctttccggc gaaggaggaa
    13621 gcgttcttcg atcttcgccg tgagcttctc catcagattg atgtttgatg acccggcatc
    13681 gggtcccgca cgccgctaga agttatatct caagcgtgca ccgtacattc gtggctcccc
    13741 cgtcacccgg aaatccgctc cgatgctcgg attgtcgtag aagcccgtga tgttgaccgg
    13801 gtattcctcg tccgtgacat tcgtagcgaa aatcgacaca tcgaaagcac tgcccatgat
    13861 ccccttccaa ccggcattca ggttgaggag tttgcgcgag tcgagccagc cataggtact
    13921 gacgatggcg agctgctcat cgatgtacgt gtagctcgcg ccaatcgaca cgtcacccac
    13981 atctgcgggc aagggcagca ggtaattcag ggcggccgtc gccgtatgct cgggcgaaaa
    14041 gctaagcgcg ctgcccgcct ccacgtaccc ggtgacttcg tcgtagggac tgtctgacgc
    14101 gggctgcggc agctcggtct catcgagctc ggtatccagg tacgtgtacg aaagactgaa
    14161 catcaggccg tcatgaaact cgagggtcgc ctcgatctcg gcaccctgga tcgtggacga
    14221 accggcattc acgatgccgg tagtgaacgc agcctcgccc gtactgctcg agtaaccgac
    14281 ctggagctgc tggtcgctga gatcgttgtg gaacaacgcg acgttcagag taccgggaag
    14341 cggagcatcg aagcgccact tgccgcccaa ctcataggca tccacttctt cggcatcaaa
    14401 gtcctggatc ccctccgccg cgaacaggtt gacggaaccc tgccggtagc cgcgtgcata
    14461 tttcgcatac aacaacagat ccggagttgg cacgtaatcc agcccgatca gccaggtcgg
    14521 tgcgtcggtc tccttgtcca gatcctgcct gcaaccggga gccgcgacgc cggttgcggg
    14581 cgaaaggaca caatccggcc cgcttgccgg cgccccgggc gcagccctgg gaaatccagt
    14641 gtagattact tgcgagtttg ttccttcggt cttgtccacc gtgtaacgca aaccggccgt
    14701 gacgctgaac tcatcgctga tgtcgtaggt ggtctgcgcg taagcggcct tgttctcgta
    14761 ttcgatcttg ccgacactgg attgcacgaa gcccgcacca aaaacatcca gacattgcca
    14821 gaaggatgga tcgccaccct ccggaagctg cgggcaggtg agaaacgtcg tggacagcag
    14881 acccgaccaa ccatcgggcc tgctgttctc gtagtacacc cccccctgcc aatcgagtcg
    14941 gtcatccagc gctcgacccg agaactgcag ctcctcgaca aaggtcgtct ggctgctgct
    15001 cggcataccg gcgggacccg atgggtagaa cgtcaccggc gcaccgacca ggtccggact
    15061 cggaagcagg ggcgcgtatg cagccggata acggaaattg gtcccgaaat tcgcgtgtcg
    15121 tatcgtctgc tcgagatcgg cataggacag gatgttcctg acggtcaacg catcgctcac
    15181 ttcccaggtg gtcgtgttga tgacctgcca ctgctcgagc ttgctgaccg ggttctcgac
    15241 atcaccttgc gcgaccgtgt agaaatcatc cttctggctc tcgagcgtag gttggcagaa
    15301 ggcatccagc agcccgccgg gtttgcatac gaacagcccc tggatcgtgc cgttgttttc
    15361 tgaatcggta taggtggcaa tggtgtagtt ttcgacggca tccgtcatat ccacgctcag
    15421 actggcgcgg cctgcgatgt aatcgacatc cgtaaaccga ttggggccga caccggatac
    15481 attcttcatg tagccgtcgc gctgcagggt atcgacgcca aagcgcgccc gcgcgcgatc
    15541 gctcaccggc acgttgatca cgccctgcgc ccgctccatg tcgtagttgc cggcggacag
    15601 ttccgcgtaa cccccgagtt cgccggtggg cttctgcggt accagctgga tggcgccgcc
    15661 cgtcgtgttg cgcccgaaca gcgtaccctg cggccctttc agaacctgca cgttctgcag
    15721 gtcgaaaaag gcgccaggac ccgcgccgtc accggcggtg atcacggcgc caccgcgcgg
    15781 cgcgaccacg tccgcgaaat aaacaccaac cgatgccgtg gtgcggaatt cctgcgtgaa
    15841 gccgcgaatc gcgaacgacg cctggtctga gccaaaacgg gtgttggcgc tcagcgaggg
    15901 cgtgtgattg accaggtccg tggcgctgat gacgttgcgc tcatccagca tcgcctggct
    15961 gaaaaccgtc atggagatcg gaacatcctg cagggattcc tccttgcggc gcgccgtgac
    16021 gatgacctcc tcctgctgct cgatcgccgc atgggcctcg ggtaccggta ccgccaggcc
    16081 cgcgccgagc atcaccgttg gcaacatggc tgccgtgtgg aatacgtgac gcgaaccctt
    16141 tctggtgctt ttgctcattg cgctccctct ggttattaaa ttccgcatcc gccgcaatcg
    16201 aagacccgct cagagcacac cctcgtagtt cttcagcacc tcgctgccgg gcaactccat
    16261 ctgcaggtag atatcgatgt gccggatctt gccggcgccg ttgaattcat agaccgacac
    16321 cgaattcacg acgctgctga aatctcccat cctgctgcgc tcctccagtt cgaggaacac
    16381 gacattcccc gcctccgtga cgcgcttgaa cgagcactcc cagtcggatg acaccgccca
    16441 attggtgagg aaacccacat agtcctgcca gttcatgact tccttgaagt tgcccacacg
    16501 ctcgaactcg tcgatcgcga tgagctcacc caacggggcc cagcttgcga cggaaaagcc
    16561 gggtcgcccg gcgctatcca ccaggcgctt catgacctgg ctgtattcca gcaccgtacg
    16621 cgacaggccc gtgtaatttc caatgacgtc gctcacttcc tgcaaggcca tgaatctatc
    16681 ctctgctgat tgtgttttcg ttgtcgatgc tgaacgcaac gcggcatcgt cgaaggcgat
    16741 gcttcgacga tgccgggcgt caggcaggcg ttgtggtgca aggcattcag gatgtcgccg
    16801 ccccctccat atacctgtcc accaccatat ggaaacgggc gacgttgact tcctcctgcg
    16861 ccaggtgcag gtacttgagc gtgtgattgc gcaggcccag ctgctggcgc ggcatctgct
    16921 cgtaatcctg ctgcagcgcc tcgaagtact ggtagctgcc cggcacttcg acatcgatca
    16981 actgtgcgcg gtactgctcg cgcacttccg ggggcagcca catatagctg gccacgtgcc
    17041 agatgcagcg attggggtca ccgtcgggat gtggcacgga catgatcacg tgggcttccc
    17101 cggcgcggat cgtcatcatg aaattcggaa acaggaacca gccctggttg tcgctcatct
    17161 ggtcgtcact gaggccgctc acatcgagcc ccttgagggt gagggtctcg cgcgtggcct
    17221 gttgcagcag cgtgcgcccc gatacgcccg ccgggaagtt cagcgcaccg ttcgcgtcac
    17281 ggaaggcatc gaccagtttc ccgaaaatgg gcaggacttc cgccactccg ggaaaggttg
    17341 ccggcaacgc catgatgccc ttgacctcgt cttccggacc aaaccccttt tccatcttca
    17401 cttcgaacgg ggcgcaggcc agggcgtgat ctccgaacca cgcgttccgg ttcttgacca
    17461 catcgggaac gatcacggcg aggagttgcg gatgcacgcc gacgatatgg tatgcctcgg
    17521 aaaacgcgtc catgaccact ttccagttgc actccagtgg ctcgcgcaca tccatcaccg
    17581 gagtcatctc gtcgagcctg tagggcgcca gcagttctat cacctcattg cccaggaatt
    17641 ccgccagcgg cttcgccgcg ggatccgggt tgaggaagat gaaaccggcg aacgtgtcga
    17701 ccggaacttc gagaagtccg agctcgctct tgtcgatcgg accgaccagg tccggcctgg
    17761 caacgcccac caggcgtcca tcgagaccat aggaccagag gtggtagggg caggtgaaac
    17821 ggccgttgga gtgccccttc ttgtcgcggc acagcacgtt gccgcgatgc ctgcaggcgt
    17881 tgacgaagcc gcggatcttg ccgtcattgc cgcgcacgac cacgtaggac tggtcgtaga
    17941 tgcgatattc cttccagtcg ccgtttgcag gcagttcatc cacgcggccg gcgacctgcc
    18001 agaccctgga ccagatcaat tcacgctcgc gcccggcgta ttcgcgcgag gtatagcgat
    18061 ccgtcgagat gcgcatattg atcttgtcga agtcgagctc ctcccaggcc atcgtatgct
    18121 cgacccagtt tccgggcttc actgcgtgcg tagtcatgga gtcgatcctc attgtcgtga
    18181 tgcgattggg cccactctat taatgttgat ttgatcgata aatagtggtt ttatcattct
    18241 attgatgacc aaagtgaaat agtctgtcat ggatcgcctg caaaccatga agaccttcgt
    18301 gcgcgtggtg gacgaggacg gctttgccgc cgccgcacgc gcgctggagg tcgaccaggc
    18361 cctggtcaca cgacaggtcg ccgacctgga acgccacctc ggcgtcaaac tgctggagcg
    18421 cacgacccgc tccatgcgcc tcaccgaggc gggcgaaact ttcctggccc gctgccgcga
    18481 catcctgtcc gatgtggccg aggccgaggc catggtcagc cgctcgcacc aggacatggt
    18541 ggggcgggtg cggctgggcc tgccgacggt gttcggcatg gaggtgacgg cgcagcaatt
    18601 gacccggttg cacgaggagt tcccggacat caccgtggag atggcgatgc tcgatcgcgc
    18661 ggtcgatccg gtggccgagg gctttgacgt ggtcaccatg aatgcaacgc tcggcgtgtc
    18721 tgccacggca gtggcacgac cgatgctgga ggtacccttg ctgctgtgtg ccgcgccgcg
    18781 ctatctgcag cgccatccgc cgcccaccac cccgcagcag ctggcagcgc atcgttgcgt
    18841 ggcgcaatgg gcctcgggcg atggcgacca tacgcgcgag cgatggacgc tggagcaggc
    18901 cgatggcacc cgggagagcg tggatgtacc ggtggcgctg cgcaccaaca cctatgcgtt
    18961 gagcctggaa gccgtgcgtt gcggcctggg ggtgggacgg ctgacgccgc cactggtgtc
    19021 cgatgacctg gccacggggc ggctggtgcc ggtgttgccc ggctggcacg ccgggcagct
    19081 gtcgttcaac ctggtgtacc ccggacggcg catgatgccc cggcgtgtgc gccacgtcat
    19141 cgatgccatc atggcgcagg tagacgaagc cgaagcggcc tcgggcggcc ctggcagggg
    19201 ctgactgcac aaccgggcgg tcaacaggcg ggcaacggcc gcgctgcgtt cgacgcgatc
    19261 agcgcgatgg cgcagccgtc tccaaggttt cgggcgctgc gccaagactc aggagcgctg
    19321 ttgcgctcac ccccatcagg acgatggcca gcatgaggaa tggcgcgtag ctgtcgaagc
    19381 gatcgaacac ggtgcccgca gcgagagggc cgaatgccgt gccgagcgag agcgccataa
    19441 ccagcgcgcc gtacagcgca ccgaaactcc tgagcccgaa atgtttcgcg gcaagaaagg
    19501 cgatgacgtc gacctccgat cctaccgtca gaccgagcgc cgcggccgcg accgcctggc
    19561 tgagggggtt ggcgccgtcg aacaacagca gcagggccga gacgatcggc agcatgaagg
    19621 cgaccgcgcc gacaatgtgg ccgggaaacc ggtccagcag aaagccggtg ccgagtcggc
    19681 cgagaatgga aaaaatgccg acgagcgcgg cgactcctgc cgccgcgagg ggtgtcgcac
    19741 cgctgtccgt caggatcggc acgaagtgaa caaccgcgcc aatggcggtg aaagagaaca
    19801 ggccgctggc cagcagcagc ttgtagagcg tcggtgaacg cagcccttgc gacagtgtga
    19861 cacccttcaa caccttcacc gggcttgttg tagtcgcgtg tttactggcc tcgccgtcgt
    19921 gagcaccgcg gaagcacagc aacaggaacg ggaagaccag cgccgaccag attcccccca
    19981 tggccacgaa cgccgtgcgc cagccgtagg cgccgatcag ccacgctgcg agaaccgggg
    20041 caactgcggc actgagcgcg gcgccggaga gcgtgatcgc aaacgccagc ccccgcgacg
    20101 cttcgaagcg gctggcaacg gcgctggtcc agaccgtcgc ctgcacccag agcgtgccaa
    20161 cggcaacgat cgaccacagc gccacccagt tcgccgtcgt tcccgtcgtc gtgcccagca
    20221 gtgcaaacgc ccccgacatc aacagcacgc cgatcaggcc gacgcggcgc ggcccgatcc
    20281 ggtccacgag tatcccgacg gggatgcaga agatcgcact gatgaacgag gctatggtca
    20341 gtccgatcga gatctgcgcg cgagaccagc caaatgcctg ctgcagcggc tcgatgaagg
    20401 gcccgagcga atagacgtgg atgacggaag tcgagtagcc gagcgccgcg acgaacggca
    20461 gcagccagaa gtttcgccat tccgttgcgg cgtttttgga catgcccatt gccattcagc
    20521 ggcccttcca ctgcggacgg cgtttttcgg caaatgccga ggggccttcc tgcgcatcgt
    20581 cgctcatgta cgcatgctcg tgcgctgccc gcgccgcctg caatgccgcc gagcgtccca
    20641 tctcggtggc gagcatcacg gtctcgcgcc ctgccctcac cgataacggt gcgccgtcga
    20701 ggatctcgcc cgccagttcg agcgccacat ccatcaggcg ctccggttgg gcgagacggt
    20761 tgaccaggcc gatttcgtag gcgcgctggg ccgtgatcgg ctttccggtc aggatgatct
    20821 ccatcatgat ccgctgcggg atcatgtgga tcagcggcgc ggcccacggc gagccccggc
    20881 ccaccttgac ttcggtaatg gcgaacttcg cgttcgtgct cgcgacgcac aggtcgcagc
    20941 cctgggcgat catccagcca ccggcgaagg ccacgccgtt gacggccgct atcgtcggct
    21001 tggacagctc gatgttgtcg tagggcagcg ggaacatgtc gcgtggcggc acctgcatgc
    21061 ccgtttccac catctccttc aggtcgccgc cggcgcagaa ggccttctcg cccgcgccgg
    21121 tcaggatggc tacgcgcaac gccgggtcgc gctcgaaacg gttccacgcc gcacgcaggc
    21181 cctcgcgcac ctcgcgactc aggcagttgc gcgtatcggg ccggttcagc gtgatgatgg
    21241 cgatgccgtc atcgcgcgca tcgaacagta cggaatcgct catcagaacc cccgctgctc
    21301 aatggtgtgg gacgcccgct ccagttcttc gcggaaatcc ggatgggcga ttgcgatcag
    21361 gcggcgcgtg cgctcggcga gtgtctgtcc cctgagttca gcggcgccga attccgtgac
    21421 gatcacgtcg acctcgctgc gcgcggtggt gaccggtccc gagagcccga tcgtgatgcg
    21481 gctgagctcg ttccctttcg cggtcgcggg cagcgcgatg atcgaatgcc cgccggacga
    21541 tcgggcgccg gcgcgcacga agtccacctg cccgccggtg ccgcccatat aaaccgagcc
    21601 gctctgttcc gcattgacct ggccggtgag gtcaacctcg agcgccgagt tgatcgtcac
    21661 cagccgggtg aggcgcgcga gcacggacgc atcgtgcgtg taggccgtgc tgcacatgcg
    21721 gatcgcaggg ttgcgctgcg cccaggcgta gagacgcttc gtgccgatca gcgcgccgtt
    21781 gatcgacacg ccggcatcga cttccttgcg cgcattcgtg actacgccgg cctcgaccag
    21841 ttccaccagc ccgtcgccta gcatgccgga atgcacgccc agatccttgc ggtcatggag
    21901 caggcgcagg atggcatccg ggaccgcgcc gacgccggtc tggatcaccg agccgtcgcc
    21961 gatataggcg gcgcagtggc gggcaatcgc ctgatcggtc gcggagatcc tgccggtggg
    22021 cacttccacc ggcgcgcgcg aaacatgcac cgccacgtcg atcgatgcgg cggggatcgt
    22081 ctcgcccggc gtatgcggca cctgctcgtt cacctcggca atgacgacgc gggccctgtc
    22141 cacggcggcg cgaacatggt cgctgatcag tccgcagctg tgattgccgc tttcatcggc
    22201 cgggctgacc tggatgaacg caacgtcaca accgatgatg ccggcggtga tcatcgggcc
    22261 gatctggctg acatgggccg ggatcacggc cagcttgccc gctttggtca tgccgcgcag
    22321 cgcgccgatc gcccccatgc tggacaacgc gaagctcgcg gctgtctcgg gcgtgaacag
    22381 cccggaaaag ctggtagcaa tgaagaccga aagcccacca atgcccctgc cttgcgcgat
    22441 cagcgcctcg accagggtcg tcggctcacc gcaggcctgg cccatgacga tacggtcgcc
    22501 ggggcgcagg aagcgcgcga ggtccagccc cgcggcgtcg gtaatgaccg tcatcagcgt
    22561 cccgcctgcc ggagcagcag ccgggcacgc gcgaggtgtg gccgatcgag catcgcgccc
    22621 ttgtggccga tggtgccaac acccggattg gcggcgaaga gatcgacgat ctcctgcgcc
    22681 gccgccagtt cctcttcgcc cggggtgaac gcggcgttga tcacctcgac ctgcgccgga
    22741 tggattgcca gcatcccgcg ataaccgtca cggcggactt tctcggcccg ctggcgcaag
    22801 ccgtcgaggt cacggaaatc gccctggatc gtctcgatcg cggcaacgcc cgccgccgcc
    22861 gcaccgagca ggcacaggct gcgcgccagc tcgtaggtga aggagtagct gccatcggca
    22921 ttgcggttgg agctcgcgcc gatcgaatcg gcgagatcct ctgcacccca gctgagggcg
    22981 acgacgcgcg gcgcgccggc gtagctgccg gtggtgaaca tggcctcggc cgtttccgtc
    23041 accaacacga tcacccgggt cgagccctgc tcgatcccgt tggcgacttc cagcgccgag
    23101 aggtagtggt cgagcaactc gacatcagcg cggccgcgcg ccttgggcag catgatgccc
    23161 ccgggatggg ccggcatgat cgccgcaagg tccgccagcg tgtgcggacc gtccagcggg
    23221 ttgacccgga cccacagccg ttcgcggccg cgcgcatggg ccatcaggaa atcggcgatc
    23281 aggctgcgtg ccctgggctt ttccgcgtcg gccaccgcat cctcgaggtc gaagatgacg
    23341 atgtctgccg ggccggccgt cgccttttcc attttctttt cgctgtcgcc cggcgcgaac
    23401 agccatgagc gcgcgctgaa gatatcttcc tggatcgcca tttacatcac cctttccccg
    23461 acagaaaaat cacgcgcccg tatcaggccg gcctcagtaa gacttgggaa gctccagaac
    23521 cttctcggcg aggaagttca ggatcatgtg cggactcacc ggcgcggtcc tgggaatcag
    23581 cacctcgcgc aggtagcgct cgacatgata ttcctgcgca tagcccatgc cacccagagt
    23641 cagcatggcc gtatggcagg cctcgaagcc ggcctcggca gccaggtatt tcccggcgtt
    23701 ggcctcgacg ccacactcct gtcccttgtc gaacagggtc gcggccttca tcaccatcag
    23761 gttggccgcc tcgacctggg cccagcactt cgccaacggg tgctggatgc cctggttcat
    23821 gccgatcggg cgatcgaaca cgatgcgctc ccgtgcatag cgtgccgcac gctggatcgc
    23881 cgcgcgcccg agcccgattg cctcggcacc gatcaggatg cgctcgggat tgaggccgtg
    23941 aaggatgatc ctgaaaccat cgccttcggc gccgatccgg tccgcttcgg gtatgaaaag
    24001 atcattgatg aaaagcatgt tcgaaccgac cgcgtgccgc cccatcttgg ggatgagacg
    24061 atgctcgatt ttcgaccggt ccagatcggt gaagaacagc gagaggccgt cggtcttgcg
    24121 cttgacctga tcgagcggcg tggtgcgcgc aagcagcatc atcttgtccg ccacatgcgc
    24181 gttggtgatc cagattttct cgccattgac caggtagccg ccgtcacgcc tttccgcgcg
    24241 ggtcttgagc ctggtcgtat cgaggccggt attgggctcg gtcaccgcga agcagatccg
    24301 gtgctcgcca cgcagcaccg gcggaatcat ccgctgctgc tgctcctgcg tgccgaagcg
    24361 cgcgatcggc tccaggctga ataccgggcc atggatgctg gaggccgcag ccatgccgcc
    24421 gcccgcctcg gcgacggcct gcatcatgat cgccgcctcg gtgatgccga ggccggcacc
    24481 gccgaccgat tccggcatgg cgatgccgag ccagccagcc tctgccatcg actggtggaa
    24541 ctcatgcgga aagacggcat cccggtcacg ttcgagccag tactcgtccg caaaccggga
    24601 acaatgccgg agcaccgttt cccgaatgtt ctgctgatcg tctgtgagcg ataaatccac
    24661 ggtattgatc cttgctgcct gcaaattttc ccggcgtgct gctttcctgc gatctgccgg
    24721 tgctcagaca attccacccg agctgggctc gcgctgcacc gcgctgtcct ctggcgcgct
    24781 cagaccgaac tcctccagca cctcacgggt gtgggcgccg agtctcggcg cggggccagc
    24841 cacatgtccc ggggtttgcg agaaccatgt cggcacgccg gggaagcgca cggggccgtt
    24901 gggcgtttcc accgtctcga agaaaccgac cgcgtccaga tgcggattgt cgaacagctc
    24961 atcgggtgtg cgcagcggcg cggccggtat gtccagcgag cggagcaggt ccagccattc
    25021 ccgcgtcgtt cgttcccgga aggtttcccc cagcaacgcg taaacggtgt cgatctgccg
    25081 cgctcgctgc gcgagcgtgg cgaagctgtc gctggcccat gccgggcgca cggactcgat
    25141 gaacgcagac cattgcttgt cgttgtacac cagcgcggaa atatggccgt ccctggtgcg
    25201 gtacggccgg cggttgggcg ccacgacgcg cgggtagacc gccggtccga gcggcggatc
    25261 gaacatcgcg ccattggcat gttcgaccag catgaacgac gccatggtct cgaacatgct
    25321 gacctcgacc tcctggccct cgcccgtgcg ctcgcggtgg aacagcgcca tcatcgtggc
    25381 atagagcgcc gtcatgccgg cgaccttgtc ggcgatgatc gtgccgacat agttcgcctc
    25441 gccggtcagc atctgctgca cgacgggcaa accgcattcc gcctggatgg tgtcgtcgta
    25501 ggcggtcagg tccgcatcgg ggccgcggcg gccatatccg tagcagttgg tgtagacgat
    25561 cgacggattg atcgcgagga catccgcata gctgaacccg agccttgcga tcgccctggc
    25621 acgcatcgag tggatgaaca cgtcagcgtg cctgacgagt tcgcgcaacg cgctcttgcc
    25681 ggcctcggag cgcaggtcga gcacgacact gcgcttgccg cggttcacat tgacgaagac
    25741 gccgctcatc ccgggctccg gcccgacgga gatattccgg gtattgtcgc cctccggtgc
    25801 ctcgatcttg atcacgtcgg cacccatgtc ggccatgatc tgggtgctgt agggccccat
    25861 caccatcgcc gtcagatcga tcacccgcac gccggacagc ggtcctgcat tcttcacgcc
    25921 attactctcc aacgtgtttc aatccggcca ggcagtgctg cgcatggcgg cgcagcaggg
    25981 ctcattcgag ctcgcgcggc gcgccggtgc ccatgtattt cgccaggttg cggtgcaagt
    26041 tggcggtgct gcgttccatg tagggattgg gcttcaaacc gctcaggccc agggatttca
    26101 tcccttgctg cacagcggcc atgttcgaga aatcctggga caggacgtag caccagtctt
    26161 cggcggtcgc ttcgcaatac tcccactccg gctgcggttc ctcgcctttc gggtacagct
    26221 gatagacaac cgcctcgaag atgcacttgt cggggttgta gccgcagggc cgggcggtgt
    26281 agcacagggc gttattgaca gagtgcccga tctggaagtt cggaaagatc tgccaggcgg
    26341 tcccgctcct gccgacatgc gcgggatcga cggtcggcca gaccacgccg cgcgcctcgt
    26401 catcgcgccg cgccgactcg agccagtgct tgagcacctg gtcggccggc gttccttccg
    26461 gcagctcgtc cttcagccgc agcgccgcat cgacgagcgt ctgcgtcgtg ttggtgttcg
    26521 ctttctgcca ggtatagagc tgcatttcgg cggtcgagat gcgcgggtcg cccttgccga
    26581 cgcgcagctt ggccgactgg ttctcgtcca tgcccttcgg cgcgtcatag ccgatattgc
    26641 tgtgctttcc ctgcaccctg gcccagccgc gaaactcccc gaatttcatg aactccggat
    26701 gcgtcgtctg gacatggtag gtctcgttga atgcctccag ggcgaccttc cagttgcagt
    26761 cgaagatgcc ccacttgcgc cacctgccgc gcatgttctg gagctggaac gggtcgagca
    26821 tggtggcggc gggctcgaga aagtcgcgca agggctcgca ctcgcgatcc atgttgatcc
    26881 agatccagcc gccccaggtg tccacgttga ccttgctcaa cccggcacat gcgtcggtca
    26941 gcgcgccctg ccagtcttcc ttgtagggcg cgtgcgtgtt cgcgccctcg aggttgaagc
    27001 gccacccgtg gtagccgcaa atgaactggc ttctcctgcc cctggcgttc ttcgctccct
    27061 gcggaacatc gacgaggcgg cggccgcgat gcgggcagac attgtgatag gcccggatct
    27121 ggtccggcgc cgtgcgcacg atcagcaccg aatcgtcgag gatctcatag gacaggtaat
    27181 tgccgacctc gggaatgtcc tcgaggcgac ccgccatttg ccataccttg cgccacagct
    27241 tgtcgcgctc ggcacgggca tactcctcgg aaatataggc ttcgaccggg tagatcaccg
    27301 ggtacgccag atcctcggag gtctcgtcga tcctgggctt ttcactcatc gcattctccc
    27361 ctgctgtgac gggccaacac gccgacaccc cggcgcgaga gcgcatatcg cgcggaccag
    27421 cgggcaatgg tcccggaacg cccgccactc ctcccgcggt gcgcgaaccg gacaatatgt
    27481 ccggactgcc aagctcgcat tttttttcac ttggagcaat atatttgcct atttttcata
    27541 ttgattgaca tgtttgtccg gacacattct aatccgagat atgtcgggca aggcatcacg
    27601 atcatcgggc cctgcatgaa gacagcgtca cgtgtgccgc acgacggccg aggggggatc
    27661 atgagatcaa gggcggaatc cggcgcgggc cgcgcagacc ctcccggagc agcgcacggc
    27721 gcgaccggcc aggcccgcgt cgattccggg gctcccctgt acctgcgcat cgcgcgcgcg
    27781 ctcaaggaag atatcgtgag cggggcgtac atggtcggat cgctgctccc gaccgaagag
    27841 gcgctgtgcg aacgtttttc ggtgagccgt tacaccgtgc gcgaagcact gagactattg
    27901 cgtgatgacg gtctcgtgtc gtcgcgaaag ggagccggca ccgtagtcat cccgccgcgc
    27961 tcctcgggct ccgacatcca tcaggtcatg tcgatcaacg acctgctggc gttcgggtcc
    28021 gacacgcgct ttgcgatcga atcgatcagg acggtgacga tcgacgcaag gctcgcctcg
    28081 cgcagcggct tgccggtcgg cgaggagtgg ctggaagtct gtggctaccg gcatgcggaa
    28141 ggcgtcgatg cgccggtttg cagggcggag tattacatca accgtgccta tgccgcggtg
    28201 ggcaggctgc tgcagcgcca cagcggcccg atttttcccc tgatcgagga catgttcggc
    28261 cagagcatcg tcgaggtcca gcagcagatt tccgccacgc tggtttcccc ggcgctggcg
    28321 gctgccctca gggtcaagga gggatcggcg gcgctcgagg tacgtcgcag ctacaagacg
    28381 gacgacggca agattgccca ggtgaccatc aatacccacc cggcttcccg cttccagcac
    28441 gcgatgacga tgcgccgcgc cagggcatag ggcgggagcg gacctaagac cccgctggcg
    28501 agcgctgtgc cgccgcctcg cgccggtgcc tgatcacgcc gatcaccgcg ggcatcaggg
    28561 agagcaccac gatcgcgacc acgaccacgc tgaaattctc cttcaccacc ggcacattgc
    28621 cgaacaggta gcccgcgaac acgcagatcc ccacccagag cacggcgccg accacgttgt
    28681 aggcagcgaa cttgccgtag ctcatcgcgc cgatgcccgc cacgaagggg gcgaaagtgc
    28741 ggatgatcgg gatgaagcgc gcgatgatga tggtcttgcc gccgtacttc tcgtagaagg
    28801 cgtgggtctt ctgcaggtgc tcggggttga agaagcgcga cttgggatag tggaagacct
    28861 tcggcccgac gtagcggccg atcgaatagt tgaccgcgtc gccgaggatc gccgcgaccg
    28921 tgaggagcca gaacaacacc cagacgtcca ggctgccgag cgccgcgaag gcgccggccg
    28981 cgaacagcag cgaatcgccc gggagtatcg gcgtcaccac cagcccggtc tcgcagaaca
    29041 cgatggcgaa cagcaacgtg taggtgtgca cgccgtactg cgcgatgatc tcggcgaggt
    29101 ggcggtcgag atgcaggaag aagtcgatga accagtgaac gaattccatg aaggctccgg
    29161 gcggggccac gccgtgatgg cgcggcaacg ggaccaattc tagcctgaaa cgacacggcg
    29221 ccacggtggg cgcgtcccga ggcgcgcacc gccacgctgg cactggcgga ggaatggacg
    29281 ttgcgcgcca cctgccggtt aaacatgggt gcactggccg cactgatgcc gtgggtcagc
    29341 attcccgtgg atccgcattc atgcggacat cccgacctct gcattcatgc ggacaaatgt
    29401 ccgcttgaaa ccggacctac gcccgctcag acatccaggc gcttcggcag gtcgggcacg
    29461 ggttccgcgc cggtcacgat ctcgcccttg ccgagcttcg agtggcgcat gccgtagacg
    29521 aaatacagca ggatgccgcc gccgaggtag acgaggaaga agtcacgcgt ctcggcgtgc
    29581 gccatcaacg acatcagcag caggccgcac atcagcgcgc cgatcaccgg caccagcggg
    29641 aacagcggcg cgcggaacgg acggtccagc gccggctgcg tcacgcgcag gtacagcacg
    29701 gtcaggcaca ccaggccgaa ggccgcgagc gatcccacgt tggtcaggtt ggccagcgcg
    29761 tccagcgaca tgaacccggc gaagccggcg gtgacgacgc cgacgatcag cgtatcgatc
    29821 cacggcgtgc ggtacctcgg gtgcacgcgc gcgaaggccg cgggcaacag gccgtcgcgc
    29881 gccatcgtgt agaagatccg cgtctggcca tagagcagca ccagcatcac cgacgagatg
    29941 ccggcgatgg cgccgatctt caccagcacc gcgaaccacg gcatccccat cgcgttgacc
    30001 gccaccgcga tcggcgccgg cgtgtcgagc tccgcatagg gcacgatgcc caccagcacc
    30061 gccgaggtga ggatgtagag cgcggtgcag acgagcaacg agccgatgat cccgatcggc
    30121 atgtcgcgcg cgggatcgcg cgcctcggca ccggccgtcg acacggcctc gaaacccaga
    30181 tacgcgaaga agatcgtggc cgccgcggtg atgatgccgc cgatgccgta gcgcgaggtg
    30241 ttctccgccg tgatcacgtc gaggagcgcg cgcctgatgt cggcccagag gccgcgctcg
    30301 gccccctcgg gcggcggcgg cacctgctcg ggaatcatcg ggtgccagtt ggcgggatcg
    30361 acgaagaagg cccccacgca gatgaaggcg atcacgacgg cgagcttgat caccacgatg
    30421 atattgttgg ccgtggcgga ttcctggata ccgacgacca gcagcgccgt gaccgcgccg
    30481 atcgcgagta tcgccggcac gttcgcgacg ccgtgggcca cgatctcgcc gccctggcgc
    30541 accgccacac ccggcgccgc ggtcagctcg ggcgggataa tgatgccgat gtcgtgcagc
    30601 aggctcaccg cgtaactgga ccagcccacg gcaaccgtgg cggccgcgag cccgtattcc
    30661 agcaccagca gcagccccat gaaccacgcc accagttcgc ccatcgaggc ataggaatag
    30721 gagtaggccg aaccggagac cggcagcatc gcggcgagct cggcgtagca cagcgcgacg
    30781 aaggcgcaga gcacgccggt gatcacgaag gagatcatga tgcccggacc ggcgaattgg
    30841 gccgcggcgc gccccgtgag cacgaagatc ccggtgccga tgatacagcc gatgcccagc
    30901 gataccaggt tgagcgcacc caggctcttc ttcagttcgc cctgcgcgtg ctcggcatgc
    30961 atctgcgcca cggacttgcg catgaacagc cggcgcagcc cggtggcggc ggcgggggct
    31021 cgcgtatcgg ccatgagggg attctccggg gatggtggcg aaagtatagt cgcgccaccc
    31081 gcggctgcgg accctccccg caggtcgcca ttcatggcga caaatgcatt cggcaacgca
    31141 catttcgcca tgaatggcga cctacggtgg acctgcgcag gtgtcgtcat gaatggcgac
    31201 ccacggggcg acccacaagg tgaagctagg cagggccttg caggttcctg acgcgctgac
    31261 cgatcggcgg cgcgatttcg gcatcgacca gcacgtccag cagcaccggg ccatggcgcc
    31321 ggaacagctt ctcgacgccg agcgtatcca gctcgtcgag gctgcggatg cggtaggact
    31381 cgacgcccat cgcccgcgcc atcagcgcga aatcggtcgg cgggagttca ttggcggtgt
    31441 tctgcagccc gcgcatgcgc tgcccgtgct tcaccgtgcc gagcacgccg ttgttcagca
    31501 cgaggtacac cacgttcagc cccagttgtt gcgcgacgga gatctcctgg gcgttcatca
    31561 ggtagctgcc gtcgccggtg atgcagagca ccggatcgcg cggatcggcg agcttgcccc
    31621 cgaccgccgc gccgatggcc cagcccatcg cggcgaatcc catcgacacc ggcatccggt
    31681 tggccgtgac cacgcgctcg ccgcgcggat gccagtagtg aagcgcccag aagaaggcat
    31741 tgccggcatc ggcatagagc cgcgtgcgcg ggggcacctt ctggctgagc atccagaaca
    31801 ggcgccgcgg gttgagcggc acggtgtcgg tgccgcagtc ctcgacgtgc agcaggctga
    31861 tgtgccgtgg caagccgtgc gcgttgcgca gcgccggcag cgccgcgttg tcggcctgcg
    31921 cctcgagatc cgggagcccg gcactcgcct ggttcaggta ggcgaaaacg atgcgcgggc
    31981 tgcccatcag gttcagctgc gccatgtacg agcgtgccag gtgatcgggg ttgctgctga
    32041 tatgcagcag tcgccgcgac aggatggtgc tgccgtccca gccgcaggtt gcgttctcgt
    32101 cgagatttgc ccccaccacc agcacgatgt cggcttcgtc cgcggtgagc gtgcgccgcg
    32161 ccgactcgtg cccggcggtg ccgaacacgc cgcggtagag cgggtggtcc gcggacatca
    32221 agccacgtcc ggccggcgtg gtgactatcg gccagtcacg gtgctcggca tagcgcagga
    32281 tctcggccat cgcgccggcg caaccttcac cgatcacgaa cacgccgcgc cggtaggcgc
    32341 gcagcaggat gttcagccgg ttcagcagca ggcggtctgg cgtgatctcc tggccgaaaa
    32401 aggcatagcg cgacatcggg ttgcgacccg aggcgatcgg cgcgctgaag atgtcctgcg
    32461 ggatgctgag gtgcaccggc cccggcgtcg ggcccgtggc gtggctgagc gccgccagca
    32521 ggttgtgctc gagctgttcc gggtgcgaga ccagcgcgtt gtagcgcgtg cagccacgga
    32581 acatcaggat gatgtcggcg cccgatccgc cgccctcctg catcgcgccg cgaccgaagg
    32641 tgcttatcgc gctctgcccg gtgatcacca gcagcggcac gccctcgacg taggcgctgc
    32701 tgaccgccgt cagcaggttg gtgcagcccg gcccggaagt cgcgctgcac acgccgacca
    32761 ggccgctcgc gcgggcgaag ccgtcggcca tgaagccggc gccagcctcc gagcgcgtga
    32821 gcacccaccg caccccgctc gcctgctggt gccgcgccag cgcgttcagc agcggatcga
    32881 tattgccgcc cggcacgccg aacacgtacg gcacgttgag cagcgcgagg tactggacga
    32941 tgagatcggc gcagctgctc gcagcgatgc tgcgctcgtc ggcgtcggtc gtgctggatg
    33001 tcatctggcc ccgtcgtgcc tgcagttctt cgatcgccgg tgccgccgcc ccgcgtgccg
    33061 caacccctgg tcgagcgcat cgtagcgtgg cattgcagct gctacacgcc cgccctggcg
    33121 ccacggggcg acaatcgcgc caaactgtta acggcttggc acttgcgccg gcatcccccc
    33181 gtggactgcc attccgtcgc cgcagccgat atatttgcgc ccgcgcgaat caccacaggc
    33241 accaggctcc catgacttcc acccgcaaca gcagcaccgc gaaacactgg accactttcc
    33301 ggggcgcgcg cgccctcaag tcctcgcacc ccgaggtacg gcagctgaaa cgccagagcg
    33361 aggacccgag cctgcacggc aacaaggtct ggcactcgag cttcgcactt atcgactacc
    33421 tgcatcgtca tccgctgcca cgcggcgcac gcgtcatcga tgtcggctgc ggttggggac
    33481 tcaccggcat ctggctcgcc aggaccttcg gcgcgcgcgt gctcgcggtc gatgcagacc
    33541 ctgcggtcga gccctacctg cagctgcagt cacgcatcaa cggcgtgcag gtcgagttca
    33601 tggccggacg cttcgaacag ctcaatgccc ggctgctgga tggcgcggac ctgctgatcg
    33661 gcaccgacat ctgtttctgg gacgagatgg tcgacccgct gtacaagttg ctgcgccgcg
    33721 cgggcaacgc cggcgtgacg caggcgatcg tcggcgatcc gggccgcccg ccgttctgga
    33781 gcctcgcgca gcgcgtggaa cagaaactcg ggggccaggt gctcgcgcac gccagcggcc
    33841 ggcgcggcag tgcaccgaag cagctgctga tcaccgattt cgcgaagttg cgtcgctgaa
    33901 ccggccgtga gcgcgcacgc agcgggcctg ccgcgctggg agcattaccc gcacgaggcc
    33961 gacatcggcg tgcgcggcat cggcgcgacg cgcgaggaag cgttcgagca ggccgcgctc
    34021 gcgctgaccg ccgtggtcac ggacccggcc ggcgtgagcc cgggcgaagc gctcgcgatc
    34081 ggctgcgagg cgcccgacga cgagctgctg ctgctcgact ggctcgacgc actggttttc
    34141 gctatggcca cgcgcggcgt ggtgttcgcg cgctttcgcg tgacgatcac cgggcaccgg
    34201 ctcacggcca ccgcctgggg tgaacgggtg gaccgcacgc gccaccagcc ggcggtcgag
    34261 atcaagggcg cgacctgcac cacgctgcgc gtggcactgg aggacggccg gtggatcgca
    34321 cagaccgtgg tcgacgtatg aaaggcctgc cggcgtgaac gacccggcgg agatgtcccg
    34381 atggacctga atctgctgca acggcgaggc gaattcgcct gggaaatccc ccgccacggc
    34441 gcgatgcggg tgccgggcgt gctctacgcg agcgaaccac tcgtgctcga tatggatcgc
    34501 aaggtctacg agcaggtggt gaacgtcgcg acactgccgg gaatcgtgca ggccgcctat
    34561 gccatgccgg acgcgcactg gggctacggc tttccgatcg gcggtgtcgc ggccttcgat
    34621 gccgacgccg gcggcgtggt gtccgcaggc ggtgtgggct tcgacatctc ctgcggcgtg
    34681 cgctgcctgc acaccggcct cgacttcgac accctcatga cacagcagcg cgcactcgcc
    34741 gacgtgctcg cctgcaaggt gccggcaggt ctcggcagca cgggagcgat ccgcctcggc
    34801 gccagggaga tggacgacat gctcgccggc ggcgcacgct gggcagtgga acagggctgg
    34861 ggcgaggacg ccgacctcga acgcatcgag gagcagggcc agatgcgcga cgcgcggccc
    34921 ggctgcgtct cggcacatgc gcgcgagcgc cagcgcgacg agatgggcac actcggctcg
    34981 ggcaaccact acctcgaggt gcagcgcgtg gcgcaactct tcgatgccga ggccgccgcg
    35041 gtattcggcc tgcgcgagaa cgacgtggtg ctcagcatcc actgcggctc gcgcggcctc
    35101 ggccaccaga tcggcaccga gttcctgaag cggatggtcg tggcggcgcc tcgtcacggc
    35161 atcgcgctgc ccgaccgcga gctcgcgtgc gcgccgatcg actcgcccct tggccaggag
    35221 tatctcggcg caatgcgcgc ggcgatcaac tgcgcgctgg ccaatcgcca gatcatcacg
    35281 cacctcgcgc gcgaagcctt cgcgcaggtg ctgccccggg catcgcttcg cctgctctac
    35341 gacgtgtcgc acaacacctg caagctcgag caacacacgg tcgatggcag gccgcgccgg
    35401 ctctgggtgc accgcaaggg cgcgacgcgc gcctacggtc ccgggcaccc ggacattcca
    35461 ccggcgctgc gctcgatcgg gcaaccggtg ctgatcggcg gctcgatggg caccagctcc
    35521 tacgtgctgg ccggcacccg cgagggcgaa gcgtgctcct tcagctccgc ctgccacggc
    35581 gccgggcgcg cgatgagccg ccaccaggcc acgcggcagt ggcagggccg cgcggtgatc
    35641 gaccagctcg ccgcgcgcgg catcctgatc cgcagcccct cggcgcgcgg cgtggcggaa
    35701 gaagctcccg gtgcctacaa ggacgtcggc gcggtggtcg acgcggccga ggccgcgggg
    35761 ctggcgcgca aggtcgcgcg gctcgaaccg ctggtgtgca tcaagggctg aagagcgccc
    35821 ggaaagccga catcgcgaaa atgcccgggc agaccgccgg gaagcccgcg cacgacatca
    35881 cgctcgcggc gctggcgctc gatcggccaa gcgcagtaca ggccccgtca gggcttgtcc
    35941 tgtcgctccg gcgcggattg cggtgccgtt accggccaga agtggaatgt cgaaaacgtc
    36001 gccgcggaac aggcacccga cttgcgttgc tgccgctgac ccttgccgct gtcactcgga
    36061 gccttttccg gttttccctg cttactgttc gcgctgcctg catccatccc catacccctt
    36121 cttttactac ggtttcatga tcgcctgatc cgttgcagca atcgttgtgc tggatcgacc
    36181 atgggcggcc ttgagtatcg aagctgagcg gattcgcgcg aggccgattg ccgggactgt
    36241 gcgccggatc acgcaatcgc cggacaaatg ccgcccccgg agcaggcggc gcgcggcggc
    36301 gggtgcacgc aggggcgggc atggcccgct agcgtcggga tgcccccggc acccgccctc
    36361 gcggggcttg agccggcgcc ccgactgagg caccgtgagc acgcgccggc gtcacaacgc
    36421 actgtctcct catcaccgcg agaatgggcc ccgcatgcaa atgatcttcg ccgctgccgc
    36481 tttggtcgcc accctgatcg gatgcgcagg ggcagtaacg gggcaactgc ccctcgacta
    36541 ccccgacacc ccgcgcggcg acgtcgtcga gcactaccac ggcacgcgca tcgccgatcc
    36601 ctaccgctgg atggaagacc tcgatgcccc ggcggtggcc gactgggtcg gcgcgcagaa
    36661 cgccgtgacc acgtcctatc tcgccgcgct gccgctgcgg gcccatttcc gcgagcgcct
    36721 cacggcactg tgggactacg cgcgcacggg tatcccgcag ctcgaaggcg gccggctgtt
    36781 ctactcgcgc aacagcgggc tgcaacgcca ggcgcccgtg tacatgcgcg ccgggctcga
    36841 cgccgagccc gaactcgtgc tcgatcccaa cctgatctcc gccgacggct cggtctcgct
    36901 ggcgcagtac acgccctcgc ccgatgcgaa gctgctcgcc tacgcgctgg ccgagggcgg
    36961 cgccgactgg cgcacgatcc gcctgcgcga tatcgcctcg ggccgggatc ttgccgacga
    37021 ggtgcgctgg atgcgcttct cgggcatcgc gtggacccgc gacggcaagg gattcttcta
    37081 ttcgcgctat cccgaacctc ccgcgaacaa ggtgctggaa gcgccactgt cggggcacgc
    37141 gttgtactac caccgcatcg gcacgcagca ggccgatgac gtgctggtct accaacgccc
    37201 cgacctcgcg gggtgggtgc tcggtggcag cgtcaccgag gacggccgct acctgctgat
    37261 cacgctggcc aagggcgcgg gcaacaacaa ccggctgcat tacgcggacc tcgccgatcc
    37321 gctggcgccg cgcctcgacg cgccggtgcg cgcgctcgtc gagagcgacg acgccgagta
    37381 cgcgccgatc ggcaacgtgg gcgcgcaaat ctacctgcgc accgacaggg acgcgccgaa
    37441 tcgcaagatc gtggccgtgg acacgcgcgc acccgatccg gcgaactggc ggaccgtcgt
    37501 gcccgaacag gctcatgcac tggaaaacgt ggcactcgtc ggcgggcgcc tcgccgcgca
    37561 atacctggtg gacgtgagca gccgtctgcg cctgttcgcg ctcgacggta cgccgcagca
    37621 agagatcgat ctgcccgggg tgggaagcgt gttcgggctg agcggacggc aggatgcgcg
    37681 cgatgtctgg tacgccttca gctcgccgct gatgcctacc acggtctttc gctacgatcc
    37741 tgcaacccat cgcagcaccg ccttcgagcc gcccgaggcg ccgatggatg ccagccgcta
    37801 cgagaccacc gcgggcttcg ccacctcgcg cgacggcacc cgcgtgccct tcttcatgac
    37861 cgcgaggaag ggcttgcgcc gcaacggcgc caaccccgtg atgctctacg gctacggcgg
    37921 cttctcggtg agcaccctgc ctgcctaccg cgccgacgtc ccggcttggc tggaactggg
    37981 cggcgtctgg gtgacggcga acctgcgcgg cggcgccgaa tacggcgagg cgtggcaccg
    38041 cgccggcatg ctcggcaaca agcaacgcgt gttcgatgat ttcgtcgcgg tcgcgcaaca
    38101 cctggtgagc gagaacatca cgtccccggc gcgactgggg atgatgggcg gctccaacgg
    38161 cggcctgctg gttgcggccg tcgccaacca gcgacccgat ctcttcgccg ccgcgctgcc
    38221 cgcggtgggc gtgttcgaca tgctgcgcta cgaccgcttc accggcggca agctctgggt
    38281 ggaggagtac ggctcggccg gcgatgcggc gcagttcccg ttcctgctcg cctactcgcc
    38341 gctgcacaac ctgaaaccgg gcacctgcta cccggccacg ctggtcacca cggccgacca
    38401 cgacgaccgc gtcgtgccga gccattcgtt caagtacacg gccgcgctgc aggccgcgca
    38461 aggctgcgcc cgcccggcac tggtccgcgt ggaggtcgcc ggctcacaca actaccgtcc
    38521 caccgacagg ctgatcgccg agcgcgcgga ccaatgggca tttgcctccg aggcgctggg
    38581 gctgcaccgg ccctgagcgc cacgagcgat cgccgacttg tgccgcgcac tcatgcccgc
    38641 gccgcctcgc cgcgcacagg gcgcagcacc agataaccca gcgccgaaag caggaccgcg
    38701 cacgtcatcg cgagcgcggt gggcaccagc gtgccgtcgt aactctgccc gaccacgtag
    38761 cccgaggttg cggccatcgc ggtctgcacg aaaccgaata ccgccgaggc accgccggcc
    38821 gccatcggaa attgcgagat cgcgcccatc tgtgagttcg acaggcacag gccgctgccg
    38881 agaaagcaga ggaagatcgc gagaatcacg gcgagcagcg gctgcgcggg cccgaaggcc
    38941 agcgcgcaca gcaggccgat gccggccagg ttcacggcca cgcctcgccc gagcgtgcgg
    39001 cgaatgccga tgcgcgtgac cagcaccgag ctcagaagcg cgccgcccat gtagcccgcg
    39061 acgcacagca tgaaggtgta gccgaaatgc tgcggcgcca cgccgagcat ctcgatcatg
    39121 atgaacgagg cgctggagat gtaggcgaat gaggatgcga aggccgcgcc gccacacagc
    39181 tggtagccga cgaaggcgcg gctgcgcagg caggtgccga actgcccgaa cacgcgccac
    39241 aggtcgagcg gcccgatccc gatcgaggga cagctctcga cgagggcgcg caggcccgcg
    39301 agcgtggcca gcccgaacag cagcatgccg gcgaactgcg cgcgccagtc gaagcagtac
    39361 agcagccagc tgcccagcac gggcgcgaac agcggtacca cggccatggc cgcggcgagc
    39421 agcgccatca tccgcgcggc atcctccgcg cgatagcggt cgcgcacgat cgcccgcgcg
    39481 atcaccggcc cggaggcagc cgcgagtccc tgcagcaggc gtgcgccgag cagcgtggtg
    39541 atcgagggcg cgagcgcgca cagcagggtg gccagcgcgt acaaccacag tcccgccgcg
    39601 accacgggct tgcgtccgtg gtaatccgac agcggaccga acagaagctg gcccgcggcg
    39661 aagccgacca tgaacaggct cagcgtgagc tggccctcgg catggccgac gccgagatcc
    39721 gccacgatgc ccggtatccc ggagagatac aggtcggtgg ataccgcggc gaacgccacc
    39781 agcgcgatca gcgtggcgcg cgcggacaac tcacgcatcc gcgctcgttc cagtgctgcg
    39841 ccgactcatg tgcgcgcgga cgccgtcccc atcaggactc cagcaccagg gcttccagct
    39901 gccggtgccc catcaggccc ggctgctggc gcttcagcac gccgtcgcgc aagacggcga
    39961 agttgggaat gctgcgcacc ccgtagcgct gcgccagcgc gggctgctcc tcggtgttga
    40021 ccttcagcac cagcgcgcgg ccggccagat tcgccgccgc ctttgccacc tcgggagcgg
    40081 ccatgcggca gggaccgcac cacggggccc agaaatccac caggaccggc acaacggccc
    40141 cgccgatgat ctcgtcgaac atcgcggcat ccgcctcgaa cgcttgcgcc ggcggcggca
    40201 gcgcggcctt gcacgcgccg cagcggccgg tgtcgcacaa ccttgccgcg ggcacccggt
    40261 tcgccgcgcc acacgcggca catttcctga tcatcgggca ccctgcgcgc cggccggcca
    40321 gtcgaccacc accgcaccac gcaactcacc cacggcaaag cccgtggcgg cgtcggcggg
    40381 atactgctcg cgcaacgcct ggcgcaccgg ctcggcaatg gtggcgccat ggcacaccag
    40441 gcacggcggc tgcgtcacga tagccgccat gaaacgcgcg ccgccatcgg gcgtgggcgc
    40501 gaaatattcc agttgccccg gtgcgtcgcc cgcgccaagc cgttcgctga actgttccag
    40561 cacggcccgg ccctgctcat ccggcgcatt ggccgggttg cgcagcctga gcgcggtgcg
    40621 cgacacgcgc acgccggccg cattgcccgc ctgcgcggcg attgccgggg cgcgcgtgcg
    40681 gcacaccgcg agcgcgccca ccgggccagc cgtcgccatg gcctgcgcca gttcgccctt
    40741 cagcgccgcc tgcatgccgc tggcgatgtc gcgcgatgcc gccagccgcg catcctcggc
    40801 agcagcggca cggccgagca ccgcgacgcc gaggatagtg aacaagacca tcgttgcacg
    40861 cacgggtatc gtctccccta ttcgggccaa tagttgcgca gtttaacgcg gctacaggat
    40921 tgccgggcca tcgcgcccgc attagattgg caccaatgac tcttcacccg gataccctcc
    40981 gatgacagct acagcagttc gcgaccagtt tcagaccctt gtcgccgata cccgggagat
    41041 ggggcgccat gccgagagcg cggccgccat gctgaagtgc ctggccaacc cgcaccggct
    41101 gatgattctc tgcaccctgt gcgaaggcga gttgtcggtg ggcgcactga acgcgcgcat
    41161 ccccctgtcc cagtccgcgc tgtcgcagca cctggcggtg ctgcgctcgg agcgcctggt
    41221 cgatacgcgg cgcgaggccc agaccatcta ttacagcgtg cgccctggcc ccgcgctcga
    41281 cgtcatccgc gtgctgcacg agcatttctg cgccgaatcg actgaaccac ccgacgcggg
    41341 gacgccgcat tcgtgacggt gctggccgtc gcgcggctcc cgggcgcttg cgcgcaggct
    41401 agctggcgct agattcgttg cgcgtgcgcg cacccgtcag caaccgttcg ctggcgatga
    41461 tcgctcgcgt atgcgagccc tgcccgatca ggccgcccgc atcccacgcc tgcagttcga
    41521 agccgtagag ctttccctgc atgccggtga acgtcgcctc ggcacgcacc ctgaccccct
    41581 tcggcgtggc ggcgaggtgg gtgacattca cgttcacgcc gaccgagagt tgccccgggg
    41641 ccagcagtgg cttcatgcaa cgcgccgccg cgagttccat cagtgcgacc atgcgcgagg
    41701 tcgcgaacac ctcgggaaat tcgtcttcca ccgacagcgc gagcgcgctg gccatgtccg
    41761 cctcgctcgc caccagttcc gcgctcgccc gcgcccccgc tttcatcgcc ttcatcctcc
    41821 ctacgtttcc ttgcgcagac gcttgccggc accctggcgc tacgcgctca cctcgaccgg
    41881 cggcggcagc gtcacctcca tcaccggcac gaactccggc atgccaccga ggctgacggc
    41941 acatttggag ctcgcgttga atgtggccag cgccaccgcc atgtggcaga tctgctcctc
    42001 ggagaactgc tcgcgcagcc gtgcgatcaa ctgcggatcg aaatgcccgg gatcgcgcag
    42061 gtacacatcg gcgaaaccga gcaccagttt ctcgagctcc gagagcccgc tgtcggcgaa
    42121 accgtcgtcg atctgcgcga ccttgtcctc ggtcagcccg tcctggcgcg ccacgtcgta
    42181 gcgtgcggcc ttgcagaaca cgcagttcac ggcgcgcgca ttgcgcaggc gcagcatctc
    42241 gaccagggcg aggcgcacgc tgttgcgccg ccacacgctg tcgttgagcc gcacgatcgg
    42301 gtcgatcaac tgcgtcgcca gccccatgct gctgtcgcgg atcacgttgc cggagacggt
    42361 cgccggcgtt cccgcccgtg gattgccctt gcccatcgtt gtgccctcag tgtgccgtgg
    42421 cggcgccgag cgccgtgaac atcagggcga gacggatgcg cccctcgatg aaacccagcg
    42481 cctcgatcag gcacaccagt cccgcctcgc cgaaatgccg cttcacgccg tcggcgagct
    42541 catcggtgag cgcgcccgga tcctgtgcgt agatctcggc gaactccagc accgcctgct
    42601 ccgcggcatc gaagtacgcg ctgccatgat agcgacccga gaggaccgcc tcgatgcgcc
    42661 cgggatcgcg ctcgaggcca cgcagcacgg ccgcttcggc gctggcgccg tgcaagcgcg
    42721 ccagtcgcag ccggcagagt tcgagcagcc gcgccggcac gtggtcctgg cgccacaact
    42781 gctcccacag cgcgttgaaa tcgcgcgcca tcgcgccgcc gggggcgagc gcgacggaca
    42841 gggtctcgct ggtcactgcg ccatctgcct gcatctgtag ctcctcttcg gtgtggcggc
    42901 gcggcggctg gcgcacggtc gcgcgccagc cgttggcgtc atggagcggc aaagctccca
    42961 tgcgcatccg ccaacgtcaa gcggccgctc ccgtgccgtg gtcgccgcga cgccggctct
    43021 gctatgctcg cgccgcggct gctcgctggc gggtggggcc gcggcaccgc gagcggagga
    43081 ccgtatatgc gctggaagga tgaacgtcgt agcgagaacg tcgaggaccg ccgcggccag
    43141 agcgcctctc cgcgccgtgc cagcggtgcg ccggtgctgc tgcaactgct gcccatgctg
    43201 ctgcgctcgc gcggcggacg ctacctgctc ggcatcctcg cggtggtggt gatcggcgcg
    43261 cagttcctcg gcgtcgatat cctctcgctg ctgacgaccg gcggacctgc cgtgcaggcg
    43321 ccctcgggcc agcgggccgc gccctcggcc gcggagcagg agcgcgcgga gttcgtcgcg
    43381 catgtgcttg ggagcaccga ggatacctgg cgccagcagt tcgccgggct cgggcgcacc
    43441 tacgaagatc cgcgcctggt gatgttcagc gccagcgtgc agtcggcctg cggctacgcg
    43501 caggccgcga tggggccgtt ctattgcccc gccgaccaca aggtctatct cgatctctcg
    43561 ttcttcgacg aattgcacca gcgcttcggc gcgccgggcg atttcgccca ggcctacgtg
    43621 atcgcccacg aggtgggcca ccacgtgcag accctgaccg gcatctcggc gcaggtgcag
    43681 gccgcgggcc gcggcgcctc gaaagcgcag gttaacgcgc tgtcggtgcg ccaggaactg
    43741 caggccgact gcttcgccgg cgtctgggcc caccacgccg agagctcgcg gcagatcctc
    43801 gatccgggcg atctcgagga ggcgctgggc gcggccaccg cgatcggtga cgaccggcta
    43861 cagaagcagg cgcagggcta tgccgtgccg gacagcttca cgcacggcag ctccgagcag
    43921 cgcgtgcgct ggttcaggcg cggcttcgaa tccggggata tcggcagttg cgacaccttc
    43981 gcggcgcaga acccctgatg cggtccgcgg gcacaaggcc cagatgggct acgccgcagg
    44041 gtgaattttc aaccgcctga gtcgcggcct ttcgtttgaa ttccacccgt ccctcgctag
    44101 gcttgctgag tcgaacaccc gccgcaaatc acagggacgc gaatggatcc catgctctac
    44161 atggccgccg ccgtcggcgc cgctgccgcc ctcgcccttg ccgccaccgc gatcctgacg
    44221 cgcggcgtaa agccccaggc gggcgcggca aaccccggca gtgcgccgcc ggaacggcgc
    44281 cgcgcggtgg cgaccgatac tggcgccggc atcgcgccag ccctggcgat ggtggcaacg
    44341 gaagaacccg cgccgcttcc cgacgccctg aacggcttct ggctcgccga cgagcacgag
    44401 atcaccaccc agcgcagcac acagctcgcc acgcagttgc gccacgtcgc gatgcccccg
    44461 cgcgaactgc agcacttcat ggcgacggac atcatggcgg gcaacgcggc ccaggaactt
    44521 tccgagctgg tgatgagcga gccgcgactg gcggcgaagg tgctggggcg ggcgaactcg
    44581 cctttctacg ggctgcagtc accgatcgcg agcgttgccc acgcgatcac ctacctcggc
    44641 atgaacacgg tgcgcaacat ggcgctcagc ttcgtgctgc aggaatcctt cgcggtagag
    44701 gatgccgcgc tcggccgcta ttgcgacagc gtctgggagg cgggacggat tgcctcggaa
    44761 ctctgctccc tgctcgcgcc ccgcctcggt ttccccgata cgggcacgct cggcacgcag
    44821 acgatacttt cctttctcgg cgatctcgcg gtgccgtcgc tgctcggcga cgaagccggc
    44881 gcgatccagg aactcgattt cgtcgaacgg ctccggttcg agcaggagca gctcggcgcc
    44941 agctcggtgc tgctcggcac gatcgtgatc cgcgcgtggg gactgccggc gcccatcgtc
    45001 gatgcggttg cggccgcggg acgcgtcgtg gtgacacccg tggagcaggc cgatcctgcc
    45061 caggcgccgc gcagcgcgct gatctacagc tgcgcgcgca tcggcgaggc gatcgcgcgg
    45121 cagcgcatcg caggacccga tgagatcgag ctgaggaatg ccgccgcgcc cgagctgcac
    45181 cacctgcagg gctacctgcg cctgccgcaa ctggcgcggc tacccgaact gctgcacgcg
    45241 cccgacgtgc ggctggcact ggcgcgcacg atcgcggccg tgcgcaacgc agcggcgccg
    45301 ggctgaggcg acatcgcgtc gggcccgcga cgcgctgcct gcggcaatca gggctccagc
    45361 agtgcgcgca tgacccgctg catcacgcgg gcggtggcgg cgcgatccat cccgggatga
    45421 cgcaccccgc gaatctgcag cccttcgtaa agcgccgaga gaacttcgca gcggccctcg
    45481 ggatcgctga gcctgtcacc gtggccgctg gcaatgcgca gggtttcgcc gaccttgcgc
    45541 cggacctctt catctgcctg gcgcaccaac gccgccaccg ccgggttgcg cgccgcctcg
    45601 gccagcacct ccagcagcag cggaccgcgc gggccctcca ccgcgcgctc cagcccgtgc
    45661 ggcacgtgct cgaccatggc gcgagcccaa tcctcgtggc tgcggatctc gtcgtcaatg
    45721 gcgaggatct gctgctgatc gccggccacg atggccgcga tgatcgcttc cttgttggcg
    45781 aagtagtgat agatgtgccc gacgctcatc cccgcttcgc gcgagatctg cgccatgctg
    45841 gcaccgtgga agccatggcg ctggaagcac gccgcggcgg ccgtaagaac ctgctcgcgg
    45901 cgcatctgcc cccgatcctt gtcgcaacac tcggcaccca tggtattaat tctcccctcg
    45961 ccacgctgac agccagcgta atggtagctt gacgcgcagc atacacctaa atagaatgaa
    46021 cgctcaatct atttgttggg gcgtacccga taccccctgc cccgaggagc gttccgatga
    46081 tccagagcct gcagcgtgcc accatgctga cagcccttgt cctgctcgcg gcctgcggca
    46141 acgacggggc cgggaccccg gccgcgccgc cgccacccga agtgggcgtg ctcacgatcg
    46201 agccgcgtga attgcagttg aacgtggaac tgcccggccg cacctcggct tacctcgtgg
    46261 ccgaagtgcg cccgcaggtg agcgggatcg ttcgcgaacg ccggttcacc gagggcgcgg
    46321 aagttgtccg gggcgccccg ctctatgtca tagaccccga cacctaccag gccgaggtag
    46381 ccaccgccga agcggccctc gcgaaggccg aggctgccgc caacaccgcg cggctgcgtg
    46441 ccgagcgctt tcgcgcgctg gtggccaggc acgccgtgag ccagcaggat gccgatgacg
    46501 ccaacgccgc cgagcagcag gccgtggcgg aagtggcgca acagcgcgcc cagctggcac
    46561 gcgcgcgcat cgacctgcag cgcacccgaa tcaccgcgcc gatcagcggg cgcatcggca
    46621 agtcggatgt cacgcccggc gcgctggtga ccgccgacca gccccaggcg ctcgccaccg
    46681 tgcagcagct cgacccgatg tacgtggaca tcacgcgctc cagccaggac ctcctgcggc
    46741 ttcgtgacga catgcaggcc gggcgcctgc agggcgcgca gagcgacgcc gcggtggtta
    46801 cgctcgtgct cgaggatggc agcacctacc cgcacccggg tcgtcttgcg ttctccgacg
    46861 tgacgatcga tcccaccact gccagcgtga ccctgcgcgc cgtgttcccc aatcccgagc
    46921 agcggctgct gcccggcatg tacgtgcgcg cgatgcttgc cgagggcgtg cgcgccgagg
    46981 cgatactgct gccgcagcag gcgctgatcc gcgatgccca gggcaatgcc tccgccctgg
    47041 tgctcgacga gaacgccacc gcgaccctgc gtccgctgca ggtgggccgc agcgtggacg
    47101 gcaactggct ggtcgagtcc ggcctgcggc gcggtgaagt cgtggtgctc gacgggctgc
    47161 agaaagtgca gcccggcacc cgcgtggtgc cggtggcccg gacggtcgcg ccgatggccg
    47221 tcgccgcaag aacgacgcac tgatggccgc cttcttcatc gaacgcccca tcttcgcgtg
    47281 ggtcatcgcg atcgtcgtga tgctcgccgg cgcgctgtcg atacgcacgc tgccggtcag
    47341 ccagtatccc gagatcgccc caccgtcggt gagcgtgagc gcgagctacc ccggcgcctc
    47401 ggcgcgcacg ctcgaggaca ccgtcacgca ggtgatcgag cagaacatga aggcgctcga
    47461 cggcctgctg tacatgtcct cgaccagcga ctcggccgga aacgcctcgg tcatgctgag
    47521 cttccagccc ggcaccgaca tcgacatcgc ccaggtgcag gtgcagaaca agctgcagct
    47581 ggccacgccg atgctgccgc gcgaggtgca gcagcagggg ctgcgcgtga acaaggcgat
    47641 gtcgaacctc ctgctggtcg tggccttcta ctccgaggac ggcagcatgg ccgagctgga
    47701 gatcgccgac tacgtggtct cgaacgtcct cgacagcatc agccgcgtga agggcgtggg
    47761 tgacatccgc gtgctcggcg gcgcacaggc cgcgatgcgc atctggctcg atccggccag
    47821 gctccacagc ttcgcgctga caccggccga tgtgcgcgcg gcgatcgagg cgcagaacgc
    47881 ccaggtctcg gcgggcgagc tcggcggcac gccggccatc cccgggcaac agatatcggc
    47941 caccatcagc gcacagtcgc gactgcagac gcccgagcag ttcggcgcca tcctgctgcg
    48001 cgtcaatacc gacggctcga cgctgcgcct gcgcgacgtg gcgcgcatcg agctcggcag
    48061 cgagacctac agcgtgcgca gccgcttcaa cggcaagccg gcggcgggcc tcggcatcat
    48121 gcagcaatcc ggcgccaatg cgctggagac cgccgcggcc gtgcgtgcgc gcctcgccga
    48181 gctagaggcg cactttccgc cgggcctgca cctcgcggtc ccgcacgaca ccacgcccgt
    48241 ggtgaaggaa tccatccgcg gcgtggtgat cacgctgctg gaagccatcg ggctggtctt
    48301 cctggtgatg ctgctgttcc tgcagaactt gcgcgccacg ctgatcccca cgctcgcggt
    48361 gcccgtggtg ctgctcggca ccttcgcggt gctggcggcc ttcggcttcg gcatcaacac
    48421 gctcacgatg ttcgcgatgg tgctggcgat cggcctgctg gtcgatgatg cgatcgtggt
    48481 cgtcgagaac gtcgaacgcg tgatgcgcga ggaaggattg cccgcgcgcg aggcgacgcg
    48541 caaggccatg ggacagatca gcggcgcgct ggtcggcatc gcgctggtgc tgtcggcggt
    48601 gttcgtgccg atggccttct tcggcggctc caccggcctg atctaccgcc agttctccat
    48661 cacgatcgtc tcggcgatgg tgctgtcggt gctggtggcg ctgatcttca cgccggcgct
    48721 gtgcgccacg ctgctgcgcc cgcacgcggg cggcgaatcg gcgcatggcc tcttcggctg
    48781 gttcaaccgc gtttttgccg cggccaccgg gcgctacact gataccgtcg ccgcgtggct
    48841 gcgccgcggc ctgcgctacc tggtgctcta cgccgtgctg ctggctgcgc tggcactgct
    48901 gttcatgcgc ctgccgacgg cctttttacc cgaggaggac agcggcttca tcgtcacgat
    48961 ggcgcagctg ccgccgggcg cgacgctgga gcgcacgcag aagaccatgg aacacatcag
    49021 ccgccactac ctcgagaacg agagcgcgct ggtcaccaag gtgttcgcca ccagcggctt
    49081 cagcttcgcc ggcagcggcc agaacgccgg ccaggccttc gtcgagctga gtccctggga
    49141 agagcgcacc gatccgtcgc agggcgtgca gacgctcgcc gagcgcgcgc ggcaggcact
    49201 gcggtccctt cccgacggca tcgcctttcc catggttccg cccccggtgc gcgagctggg
    49261 caacgccacc ggcttcacgc tgttcctgca ggaccgcggc ggactgggcc atgccgcgct
    49321 gatggacgcg cgcaaccgcc tgctcgggat ggccgagcag cacccgcagc tgcgcggcgt
    49381 gcgcccgaac ggactggagg acacgccaca gctgcaactc gacatcgacc acgacaaggc
    49441 cagcgcgctg ggactctcgg tcgccggcat caacgacgtg ctctccacgg cctggggcgg
    49501 gcgctacgtc aacgacttca tcgaccgcgg tcgcgtgaaa aaggtctatg tgcagggaaa
    49561 tgccgaagcg cgcatgcagc ccgaggacct ggaaaaatgg tacgcgcgca acgacaaggg
    49621 ccagatggtg ccgttctcgg ccttcgcctc cgcgcactgg acctacggct cgccacgact
    49681 gacgcgctac aacggcatac ccgccgtgga gatccagggc caggcggcgc ccggagtaag
    49741 caccgggcag gccatgcagg cgattgccgg catcattgcc gagctgccgc ccggcatcgg
    49801 cttcgaatgg accggcgtct cgctgcagga gcaacaatcc ggcggccagg caccgctgct
    49861 ctacgcgctg tcgctgctgg tggtgttcct gtgcctcgcc gcgctctacg agagctggtc
    49921 gatccccttc gcgatcatgc tggtggtgcc gctcggtgtg ttcggcgccg tgctcgccgc
    49981 gacgctcgcc ggcaaggcca acgacgtgta tttccaggtc gcgctgctga ccacgatcgg
    50041 cctgtcggcg aaaaacgcga tcctgatcgt ggagttcgcc aagacccgcc aggaacacgg
    50101 cgcgggcctg gtcgaggcca cgctggaagc ctgccgcctg cggctgcgcc cgatcatcat
    50161 gacctcgctg gctttcgtct gcggcgtgct gccgctcgcc accgccgatg gtgccggctc
    50221 gggtgcgcag aacgcgatcg gcaccggcgt ggtcggcggc atgatctcgg ccaccgtgct
    50281 cgcgatcttc ttcgtgccgc tgttctacgt gcgggtgcgg cgccgcttcg ccgcggagcg
    50341 ctccccgacc ggcaccgatt gatgatcggg atgaggtgaa ccgcagatga cgacggaaat
    50401 tgcttcccgc gagcccggtt gggcggaact gctgcgcggc cacctgcgcg agcactcgct
    50461 cgcggcactc gggctgggtt gcgcgctcgt ggccgtgctg gcgctgctgg ctcagagcat
    50521 cgcgcaggtg ctcggcggcg aggccgcgcc ggggctgcgg ctggcgctgc tgggcggcgc
    50581 ggcgggcttc gcaacaacca cgctcggtgc gctgcccgca ctggcgctgc gcggcctgcc
    50641 ccagcgcatc gaggacagcc tgatggggct cgcggccggc atgatgctcg ccgcgagcgc
    50701 gttctcgctg
//
LOCUS       DAORUQ010000003        47871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_427_length_47871_cov_6.374770, whole genome shotgun sequence.
ACCESSION   DAORUQ010000003 DAORUQ010000000
VERSION     DAORUQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 47871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47871
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_427_length_47871_cov_6.374770"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1346
                     /locus_tag="PLW13_00555"
     CDS             213..1346
                     /locus_tag="PLW13_00555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY68886.1"
                     /translation="MDFGFSEEQRDIQNLARQILKDLVSPTTLAAYDGYASERFDRAL
                     WGKLAEAGLLGVAVPEEHGGMGFGFMEVALLMEEAGRSIAPAPLAVHMAGGIVPIARF
                     GSAEQKARLLPGAASGEIVLTAALEEQFALDACEPATTARAEGGGYRVSGEKFAVPFA
                     HCAQRVLVGAMSDAGLVVLLLDPRAAGVTLTAMKTSSYEPQSLLVLDNVLVPAADVLA
                     GPQHGAEVMRIVTEHLTVALCAHQLGVTDTLLRQTAAYTSERKQFGVPIATFQAVGNR
                     AADRFIDVECLRLTTYQAASLLASGAPASVEVDIAKIWAGDVGHRASYTAQHLHGGAG
                     VDRGNAPWRYCLWARHNELTLGSTASRLASLGQRCANGEAVVD"
     gene            complement(1386..2582)
                     /locus_tag="PLW13_00560"
     CDS             complement(1386..2582)
                     /locus_tag="PLW13_00560"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008889 - glycerophosphodiester
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQY68887.1"
                     /translation="MPALAAPEVLNIQVGPRPYFLVADMDDSPLKQRLGSQDCRNKPF
                     RKTDFSIGHRGAPLQFPEHTRESYEAAARMGAGIVECDVTFTRDKELVCRHSQNDLHT
                     TTNILLVPELAVKCTTPFTPATFDAEGNRLTAANAECRTSDITLAEFRTLKGKMDAFD
                     PDARTVQEYVAGTPNWRTDLYAGASSGTLMTHRESIALFRKLGVKMTPELKSPSVTMP
                     FDGFSQRDYAQKMIDEYRQSGVRPAAVWPQSFDLDDVLHWIRSEPDFGRQAVFLDDAN
                     VTDELPDRAELAQLANQGVRIWAPPMWALISSENGALVPSAAARDARSVGLDMITWTL
                     ERSGLLAGGNGGFYYQNISDVLAQRGNEGVAMEALDVLGRQVGVLGVFSDWPGTVTYY
                     ANCFGL"
     gene            complement(2702..3160)
                     /locus_tag="PLW13_00565"
     CDS             complement(2702..3160)
                     /locus_tag="PLW13_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY68888.1"
                     /translation="MYLSAREIEEMEGVRKVHYLNPAAERLNKSLGDAVGMQHLGAHL
                     ITVDPGHQSTEYHVHHFEEECIYVLSGRGLAVLGEQLQPIGPGDFIGCPANGIAHELI
                     NDGAEPLVCLVVGQRLAHDVVDYPRLEKRLFRNAGEWNLVDQGDIEHVKR"
     gene            3257..3952
                     /locus_tag="PLW13_00570"
     CDS             3257..3952
                     /locus_tag="PLW13_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQY68889.1"
                     /translation="MCGRYNIHDDPWLQTLLRELGVEMRIPTRVNIAPTEAVPAVIEE
                     QGRRCLREMRWWLVPSWAPEIDQRYSMFNAKAETLGTSRAFRGPLRHRRCILPASSFI
                     EWTDAGGHRQPWLIRPERGAIAFAGLWDVWEKEGNHLESCAIITTDAAPGIDRLHNRM
                     PVMLRERDFARWLDVDTPLGDPSDILHSRLPHPFVLAPVSPAVNNSRHKSEALLAPVG
                     EEERVEAVGQWAK"
     gene            3967..4983
                     /locus_tag="PLW13_00575"
     CDS             3967..4983
                     /locus_tag="PLW13_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016970162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HQY68890.1"
                     /translation="MSDDRFPAPGAAALAADGSLLLGARRIPLPASVSPEARAFLAMP
                     RVTLGERPPLADKEAWRRWVTTMNLGSEPRVAQMMALVGDRVRVETRTIAGVVVHVAT
                     PAESHPSRRDWLRITVHGGGLVYMGGSYARAEAGLVALQSGCEAWSVDYRMPPDHPYP
                     AAVDDVVAAYRAALATRAPGSVAISGASAGGNIAAAAALKARDLGLALPGALGLFTPE
                     CDLTESGDSFVTNLDVDNVLPGPLPEEIALYADGADLRHPYLSPLFGDFAPGYPPTQI
                     QTGTRDLFLSNSVRMHRVLRAAGVSAELHVWEAMPHGGFGFDAPENADARREFNDFLE
                     RHLG"
     gene            5002..5487
                     /locus_tag="PLW13_00580"
     CDS             5002..5487
                     /locus_tag="PLW13_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY68891.1"
                     /translation="MATQMGNSTEMHAELLGLPPCDQVGFVVRDLQAAMAMYAPLFGP
                     FSTMDGSVKQATYRGRLADAGLQIAFGRSGNLEIELIEWTGGESPHREFIEAGREGMH
                     HIRFRVEDTDHWVERLRPLGYEAVWYKRWSADTVFAYLERPGDPTLIEFLQMPPGGPG
                     A"
     gene            5514..5933
                     /gene="arfB"
                     /locus_tag="PLW13_00585"
     CDS             5514..5933
                     /gene="arfB"
                     /locus_tag="PLW13_00585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQY68892.1"
                     /translation="MIELAPGVAVADEEIEINAIRAQGAGGQNVNKVSNAVHLRFDLG
                     ASSLDVELREALLALHDQRISKDGVIVIKAQRFRSLEKNRDDALQRLRELVARGLLRR
                     APRIATRPSRSAQRKRVDEKSRRGQIKAARAPVRRDD"
     gene            5945..6433
                     /locus_tag="PLW13_00590"
     CDS             5945..6433
                     /locus_tag="PLW13_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HQY68893.1"
                     /translation="MDPVIFLWLIAIALVAAGVAGLVLPVLPGAALLLAGLVMAAWIE
                     EFQYVGVGTLAALAVLGCLIFAVDFVAGSFGARRFGASPRAALGAALGALLGLFFGLP
                     GLLLGPMAGAVLGEISARRTWSEAGRAGVGATLGLVLGAAAKLGLALTMIGIFLFVRF
                     LG"
     gene            6457..6960
                     /locus_tag="PLW13_00595"
     CDS             6457..6960
                     /locus_tag="PLW13_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQY68894.1"
                     /translation="MNLLLVSWLTGCGWMDGPEPGPAAPPPDQRHGTLPGQVSLADPE
                     AARAALLEQYLDWAGVPYRMGGSSREGLDCSGFIQLTYLQRFGVELPRDTSAQRGVGS
                     KVAPSRLLPGDLVFFDTGPAERHVGIYVGKRQFLHVSSSTGVMLSRLDDGYWGARFRD
                     AIRVAER"
     gene            7107..7463
                     /locus_tag="PLW13_00600"
     CDS             7107..7463
                     /locus_tag="PLW13_00600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY68895.1"
                     /translation="MSERRLSNRQPSLVFYDLSFADSGERFGEMLDLSSDGLMALCDA
                     PVLPGSRVALQLDIAARMLGTRSLSFAAECRWCAPGATAGGYACGLRVLAQDDASSRQ
                     LPHLAATMVFDSLRAF"
     gene            complement(7464..9068)
                     /locus_tag="PLW13_00605"
     CDS             complement(7464..9068)
                     /locus_tag="PLW13_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HQY68896.1"
                     /translation="MTSADDEVESYIARLPPNTGFATGFRAWGLKYRWFALLTIVAGN
                     IAALLAGTIVNVAIPDIMGAFGISQVDAQWLSTANLAAATVAMLASSWMVRVLGLRQT
                     VHITTYLLLAGSLLGGLATNTGIMIVARIMQGITAGVLTPVSTMLIFQVFLPGRQGVA
                     IGITSVGAILAPAMGPAIGGVLIDTYSWRYVFYAGIPFSLLTLAASSFFMPTRQPLPT
                     RVPFDWSGMIALSVALIALLVGLSNGEKEGWGSDYILGCFAVALVSGAGFVWWQLRTE
                     HPLLDLGLFANRNFTIMTTNSFVFGAGLFASTYLVPVFLQLVQHLSPTRAGALMIPPG
                     IAMALLFPFAGKLAEVLDHRLLISGGIAFFATSFWLMSGADANTGFWALAWWLVLSRL
                     GIGLVIPALQLGALSHIEISRLSQASAAFNFMRQLGGAFGVNLMSVVLERRHIYHADY
                     LLSTQTYGSSETLNMLGLLRGMSHTLGFAGTEEWHAARAFLQGMVQQQALAAAFRDSF
                     VILAIAFLLTLIPTLALKPRKAVQGR"
     gene            complement(9055..10176)
                     /locus_tag="PLW13_00610"
     CDS             complement(9055..10176)
                     /locus_tag="PLW13_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQY68897.1"
                     /translation="MSADNQAQSAEHRLVMNQRARRRALLVAATVALGIATAWWLYQR
                     YTHVFSEDARIATSMIEVSAKVAGQVVEFPVSQGDELLPGALIAQIDDRAARLTLAEL
                     EAQFQSMESACQRLEVQIDMVDRESGGQLSSAKSHLDAVQASLASARSDLQFKQGEWE
                     RAQSLRERQIISQQQWENARNAHQQSEQLHQRALAEVESARGNVVEAEAGQSRIQVLA
                     NELRGLQHERDRIRASIDKQKVMIGDLRITSAGKGTVDETFVHVGEYVVPGQRLLLMH
                     DPQRIWVDANIRETEIRHVEPGDQVTISVDAYPDREFRGEVVRVGQSATSQFSLLPNT
                     NPSGNFTKVTQRLPVKITIEPADELLRPGMMVEVAIDIR"
     gene            complement(10176..11204)
                     /locus_tag="PLW13_00615"
     CDS             complement(10176..11204)
                     /locus_tag="PLW13_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQY68898.1"
                     /translation="MHYYLRHPMSLPDFEQNCAFLIHDTARLIRRRFGLSIRDLQLTQ
                     AKWRVLTTLMERPGISQSELAERLDIEKAPLGLALDWLEQAGWIERKADPADRRVRRA
                     ALLPRAEPALARMSERFRAIEANYLRGFDAEEVAQMLESLLVIRDALRAAAPTSASAA
                     RTVAIAAPAADTVTGTTATTGTAGTTTTNATTETYVSVLFECARLLTRRFDARLAEMG
                     FTRNQWLVMNTVYRNEGMRQTAIADATEIGAAPLGKVIDALQADGWLERRADPRDRRA
                     KRLFLTRRAHHLLSGKRQRFEQLHAALLQPLGPRRTQLLVNSLGWIRQRLLEESPQAT
                     ESRFAGAH"
     gene            complement(11303..12226)
                     /locus_tag="PLW13_00620"
     CDS             complement(11303..12226)
                     /locus_tag="PLW13_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HQY68899.1"
                     /translation="MSHMPTLRQLRYFVALVRHRHFGRAAAECHVSQSAFSIAIRELE
                     TQLELSLVDRTSKQVTITAVGQEVAVLARHTLADAEELVEFAQRERSPLARRINLGVI
                     PTIAPFLLPRVMPAASARFAHMKLYLREETTERLLASLFDGQLDLLLLALPWDIRNAE
                     VLPLFRDPFRLACRAGSPLLEKGRLRVDQLAPESVLLLEDGHCLRDHALSACRLRRPD
                     KLNRFAASSLYTLVQMVAADLGVTYLPAMAEGSSLLEGTGIETHALDNKSYRDIGLVW
                     REGSARAAEFREFGQFINECRPPGVIDLSRR"
     gene            12354..12839
                     /locus_tag="PLW13_00625"
     CDS             12354..12839
                     /locus_tag="PLW13_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HQY68900.1"
                     /translation="MKQSGIRDIDIGIPREQRAAIAQGLSRLLADTYTLYLKTHNYHW
                     NVTGPMFNTLHQMFELQYNELALAVDQIAERIRALGFRAPGSYREYAALTVITEEDGS
                     PDAIGMIERLVDGQEAVIRTARSLIPEAAEAHDEPSADLLTQRLQVHEKNAWMLRSML
                     D"
     gene            12946..14064
                     /locus_tag="PLW13_00630"
     CDS             12946..14064
                     /locus_tag="PLW13_00630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY68901.1"
                     /translation="MTEPGFERVLEAFAHNFTAHGDIGAACCVYLHGRPVVDIWGGTA
                     DPVAGIPWRQDTVQLVFSAAKGPTAVCIHRLAEQGLLDIDAPVARYWPEFAAAGKERI
                     TTRMVLSHRAGLAAVAGDLALADVLAWDPVVEAIAAQAPEWEPGSAHGYHARSFGWIL
                     GEIIRRITGESAGQHLARTVCAPLGLDYRVGLPATERARCARLVPPDADHSAAAALGA
                     NSLTVRVMTGPSGLFGYNEMWNRDDVLAAEMPSSNGVGSARALARLYAATIGEVDGIR
                     LLRPGTVREATRVVSRGPDRVILVETCFGLGFSLPPMLGPDCGPNAFGHPGAGGSLGF
                     ADPEAGLAFAYVMNRMRFEAGGDPRAASLVRALYECIG"
     gene            14100..15044
                     /locus_tag="PLW13_00635"
     CDS             14100..15044
                     /locus_tag="PLW13_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY68902.1"
                     /translation="MELTILSDFEMPGLADAIARQGAGARLLGVAADEPLPAHLPGAA
                     LLTTALGNPRLEALLAAASGLRWLHVFGTGVDSFPLHLVPPQVTITCSRGASAVPIAE
                     WVLAMMLAFEKQLPQSWISAAPARMWQANLGTLAGRTLGLAGCGGIGAAVASRAAAFG
                     MQVVALARSPRSEPPPGVEMLRTKEELCERADHLVLCLPSTPATRHFIDAAALARMKP
                     GVHLVNVARGALVDQEALRVALDAGRVACASLDVAEPEPLPEGHWLYTHPRVRLSPHI
                     SWSAPQAFAALLAGFLDNVGRFARAEALSGIVDREAGY"
     gene            complement(15058..16188)
                     /locus_tag="PLW13_00640"
     CDS             complement(15058..16188)
                     /locus_tag="PLW13_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQY68903.1"
                     /translation="MEIKTDSARPITSTRDRAHLQRDLQAWLKQKLGADARLGELSAP
                     QSNGMSSETLLFDLQLREDGVERSLRCVARLPPDAGAMPVFPEYDLLKQFRVMQLARE
                     RSSAPVPRMLWYEADASHLGAPFFVMERADGDAPPDVPPYVFDSWLLRASPGERERLQ
                     RCAVDVLARLHAIELGPADLELLRPREAGATALRRHVASQRSYYDWVVAGDGIRSPLI
                     ERGFAWLEANWPKDEGPEVLSWGDSRIGNMLFVDFQPSAVLDWEMVATGPREIDLGWM
                     IYIHHFFQDFTPLVGLPGLPDMMRLDAMSAAYAAATGHQPRDMEFYTFYSALRHGIVM
                     FRIGRRQALFGESTIPENPDDMIPHRPAIEAMMEGNYWDTRR"
     gene            complement(16311..16928)
                     /locus_tag="PLW13_00645"
     CDS             complement(16311..16928)
                     /locus_tag="PLW13_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQY68904.1"
                     /translation="MEQYADTVQLIALAAGAALASGINLYAVMLVLGLLGRFAGVELP
                     PGMELLQHPLVLGAASAMFLAEFVADKIPGLDSLWDALHTFIRIPAGGLLAAAAAAPV
                     GPDFAVASGILGAGLAAGTHATKAGGRLLLNTSPEPFSNWGASLGEDVLAIGAMWLAV
                     FHPWLFLGVAALFLLLLAWLLPKLWRGVRLLLARIARLFRAGAPA"
     gene            complement(16984..18009)
                     /locus_tag="PLW13_00650"
     CDS             complement(16984..18009)
                     /locus_tag="PLW13_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY68905.1"
                     /translation="MLLETRTKPGNVRHLIYGLNPLISLLRQHGADVEAMLAAARIPP
                     GALADPDYKLSPAQELSFTERALAQLEQPDLGLRLGPRYHLSAYGMLGLAVMTSSNLV
                     DAFGVLFRNILMTWTYMHWSLRTEDGVAIIALGKQRDLGGCHQYMIDRGLAASYTIAA
                     EALGKRLPLIEVNVAQPEPGYAQAYADFFGCPINFGAPTNDFRFPGSFLSEPLVQSET
                     ASARVFAAQCERICAELERGGSFAELIRQHLVQLPNQLASLETIAERLCTTPRTIQRK
                     LAVEDTSFKELLEDVRKNLSLEYLQATELSVEDIALRLGYADAPSFSHAFKRWTGKSP
                     RSARVAP"
     gene            18199..19464
                     /locus_tag="PLW13_00655"
     CDS             18199..19464
                     /locus_tag="PLW13_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY68906.1"
                     /translation="MSAIEWPETPRGKLTISGAEPPLPPAIAEFQKTLREFAVNVMRP
                     TGIQLDRMSAEEVIAPGSPYWDLRRKYLDLGINMETLGSFSPEDFSVAFPLIFEELGY
                     GDAGLAISMGAAILPMYLAAKFGNQFLLERFPDTLIGCWGITEPDHGSDSLDPNKQIL
                     DARGQYGRPNCVATLRGDKVIINGQKSAWVSNGPIAEVCILYCAADTGNGPDPYNGCV
                     LVLPMDAPGVSRGKPLEKMGQRALPQGEIFFDNVELSSDYILAGPVAYKKAVYCIHTE
                     ANALMGGTFTGVAQAAFDLALDYAHERRQGGVPIIRHQSVQQRLFHMYRKVEISRALT
                     RRVVLFNNTAPVPALQAAMTAKVTGTQNCFDVCSDALQLFGGNGLTREYPMEKLLRDA
                     RASMIEDGCNEILSIKGGCYLIDEERLRG"
     gene            19461..20693
                     /locus_tag="PLW13_00660"
     CDS             19461..20693
                     /locus_tag="PLW13_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY68907.1"
                     /translation="MKQNAWIAGVGMTHFGKHMDRGLKSLAGEAITAAVADAGIGLRD
                     VQAAWMGNAAAAVITGQVCVPGQMALRELGVGRIPVVNVENACATAATAFQQACTMVS
                     AGLYDVVLACGAEKLYHPDKLKTFSVFSGAVDVEAMDRVVSGIARRIEALGAPIDTAG
                     AGTTRSLFMDIYAIWALSHMQRHGTTREQFAMVSAKNSVHGSLNPKAQYRDVITVQDV
                     LAAREVSWPLTLPMCSPIGDGAAAAIIVSERKARELGMRRAIRVEASQLSSGWDFAEG
                     EESVAEGSVRALYEATGIGAPDIDCIELHDASAPSELMYYEYLGLCAKGEGGRFVQEG
                     HSRLGGSKPVNTSGGLMRKGHPIGATGLAQIVELTEQLRGEAGARQVAGAKVALAENG
                     GGYIGTDAAAMVLTLLSR"
     gene            20773..22404
                     /locus_tag="PLW13_00665"
     CDS             20773..22404
                     /locus_tag="PLW13_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY68908.1"
                     /translation="MSDERILANLIARQAAEHPDLDVLTFVDVTADGGFAEEVRSFRQ
                     LRDNGQRVAQALGDAGMQAGDRFALLMQNHPEFVDAMVGSSIAATVFVPIDPRTRGEK
                     LAFMLGFSGCRGVICADYALGHLMAVIRELVEIRWVWVLRTGAVQHLPASGVSPRWLD
                     EVLPVAVPALPVAVDSPEQTMQMLFTSGTTGDPKAILANYLRFHAVGSIAPLIGWSAA
                     DVFYTGLSLTHANAQLITLGNVLTLGARGVISRRFTKSRLWDICRRYGCTTFNLLGGM
                     TTAVYSEPPRADDADNPVRLVLSAGMPAAIWQKFAQRFGVQIFEFYGAAEGGMSFNPP
                     GVGPVGSIGKPPPTLEARIVDEDDRECAPFEQGEIVFRNADGSCPPVEYFQNPEASAK
                     KTAGGWLRMGDVGHRDAGGWLYFDYRKGGGIRHNGDFVNVAFVEKAIAEHPGVDDVFV
                     YGIEADSGVPGEKDVVAAVVPAPGAAFDVAELYRHCRAALESNFVPGYIQLMSEIPKT
                     ASEKPQERFCLEHLRTHPEAVFTESSTSEPLRRKS"
     gene            22401..23510
                     /locus_tag="PLW13_00670"
     CDS             22401..23510
                     /locus_tag="PLW13_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HQY68909.1"
                     /translation="MNAEVVVYGASGYTGKLIAWHLAERGIPFIAAGRNARRLAEQMA
                     KVPELAGARYECVAVEHEEAALAKLFSGRKVVYNVVGPFMQLGDPVVKACLAAGCHYL
                     DTTGEQDWMLHLKRSYGPDFAQKGLLLVPACSWMWLAGQLAAEVALEHPGIDSLDICY
                     LADSNTSVASTMSFLRMLTKDQFFLEHNRMCTWPQATAYPVSIPGVHRVLNALPWSGG
                     GEPVWYEDDARVRNCSVLVAFRNQAMLQAIVDILVEFEHKHKHLPVAEQEKITNELGN
                     QLVSVEPERESPALNRSLLCCHGRGDTESVSVVLRGNSPYIQTGVFAAEACRKILLGT
                     HRGKGFMSPAGAFGAREMIAAIAASGQLAWDTRTV"
     gene            23577..25982
                     /locus_tag="PLW13_00675"
     CDS             23577..25982
                     /locus_tag="PLW13_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQY68910.1"
                     /translation="MKTIRQRISAFTIRHAGKVLLLLALVTAVFAAGLPRVELRTIFS
                     DLFPSNHPFVQTFRDHPNFGNPLTVTIMVKRKDGDIYNAETLQKIWNMTRDIDLTPAV
                     DHDQVLSISTEKARFQEATPFGIDSQPLMGDHAPATAEEIAEFRRRVEKSPNSRQFLI
                     SADESATLIRATFIERLLDYGESFEYVQALVERERDANHEVYVAGQPVLTGWVYRYEK
                     QMFGIFAVTITALLLSLIVYMRNVAGVIGPLVTSATAAIWGFGFVGWLGMPIEPLIMV
                     VPLLLIARSFSHCVQFTERFYEVYHHVRDKRKAAEISMGVMMAPGTLGIVTDAAGLFL
                     IAIAPIPAMERFALFCGFWALMLIPTNVIMSALLLTFLPEPRNVAAIIGEDGKHSPLH
                     AGIKRLLGVTGSLSHGRKGRVLAVVVLVVTVLAVVKLGDLKVGNPVEGSSLLFEDSEY
                     NVAVGNINAQFPGLNTLEIVFEAKEASSVRRVMHQADTIATMNALQRIVENDETPPMA
                     TLSFADYLPEANRLFSGGNPKWAPLDPSDESVAAAAGAVLFGTSPKAFLHVTDFIQEN
                     GTVSLWYKDNKQETVDRALAQARAAVEKVGAEHENFRIRLATGTIALQQSINDTVDRY
                     QYIIMALLNVVMLFSCAYAYRSFVAGILLLIPVNLSNVLLGAVMVSMGIGLDVNTLPI
                     TAIGIGVGIDYGIYLLSRICEEYQLHESYEKAIAEAVGTTGKAIFFTATIVLIAILPW
                     YFLSELKFLADMGLLLVMVMLINMVIALLVLPLLVWLFKPKFVGSKDLLVGEGVDLSD
                     YAT"
     gene            26025..27398
                     /locus_tag="PLW13_00680"
     CDS             26025..27398
                     /locus_tag="PLW13_00680"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY68911.1"
                     /translation="MRIRKYDFNYSRRAFLDKMATGAMAAGVLSPLWPLIARAGDITK
                     AYPEELLSIEAYTKGKIKTGDLITADNVEHVKDLLDPVAYVHVSQMGRQIRIVKTTTD
                     ATRLFPRKYLEATLRNQGKARLDADGNVVTAEGKPWIGGNPFPDPKDGLEAFSNLTLS
                     WGRHDNSFYAVRDWDIGPDGDLQYEYDFCWAEQNTVALVGENGPYMPGHEDKLRFQSV
                     WFTYPNDSKGTSFLNTWYYDQRKFPDLQGYLPAFKRVRRFPTNQRFEPLVPGITLFLS
                     DAWAAGDPMLTWGNYKVIGRQPMLGAVSENWMGPDTNYERPVHGGAKGKTFMETAMEL
                     VPETIVIEAEPTGYPRAPVGKKRVWIDTRNMMYVAYVTYDRRGELWKSFEPAYSVYEK
                     GDQRLMDGAEPAWSWTHVHCHDVQTNRMSRFVQAEEVRGGFRSGFNIEGMYDKYLTMQ
                     ALLRMGT"
     gene            27398..28393
                     /locus_tag="PLW13_00685"
     CDS             27398..28393
                     /locus_tag="PLW13_00685"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY68912.1"
                     /translation="MRRRREPARSCATLLLAALCCAAARAAEPVVLHQGIVHDALYDV
                     CFDARGAVAVGAAGVVLKSSDAGASWQPQPQEATALALLGVACTDERRLAVGQAGTVL
                     VDDGNGWRKVETGTEQRLLAVAMNDSGLAVAVGGFGAVMLSRDHGESWEPVVFDWPAL
                     VEDFAEPHLYDVAVAGDGAITLVGEFEIVMRSPDGGATWARVHKGDASLFGLHIGPSG
                     VGYAVGQEGRILRSADGGASWEALAAPVDAPLLGVWASADDRVLVSGMRTMLAGSAGG
                     ASWRRLEGADIATGWYAALAPATADAQRIVSVGNAGRVLGFAPTVTAEEDDKHQP"
     gene            28405..30228
                     /locus_tag="PLW13_00690"
     CDS             28405..30228
                     /locus_tag="PLW13_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68913.1"
                     /translation="MSKPRRLAAAIRGVTLVSAALAAPAALALDLNVSGFIRQEMAYK
                     ITDDENPFNQQGNLYNGKPVDNFVHGLYGLPPEITRPDYSESNDWNLQVTRGELDVAA
                     TFSEHLTGAMKVRAYYDHGVYDDFGDVNFFEVPYRGDCATRLEVCGDDYMIDLPSLYL
                     DYTNGGFWTRIGNQQIAWGESIFFRVLDVPNGLDLRRHSFLDWASEEYSDERVPGLGI
                     RSSYRFQNNWEIEGFAQEFNPTIYANENTPYNVIASQFVVQQEETFDEVDDEWNFGAR
                     LRGQIGELGLQFIAVSRRNPDGAFRWTTSKVNPFVAAGIGAADPDLGGVTLGQLLAQT
                     PFQPFTGQGVFTAREWFDYSGMARLNGANLQQLLDDFPAAKALTGANGLPTTGVDNLA
                     TSTLVLDAFFSPILGLGDLRGHIERKFHREEIFGIGMNYMFYAEPDSLLDQLIVRFEA
                     TVTPDKMFTDPSLGQEFLEETEWATSLVFEKYQRFSETFPATYMVLQWLHKSESDLFG
                     RHLDGFDGDASRSPPAGRDQFDAIAFAMQQPSPTLMWRFDLAVLYDVEGGILVQPGVR
                     WKPSKSYTVELYANLLEGDNNDSMGTFQWSDEVGLRLGYQF"
     gene            30228..30857
                     /locus_tag="PLW13_00695"
     CDS             30228..30857
                     /locus_tag="PLW13_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY68914.1"
                     /translation="MDVLEQGMSLARLGGPVVLVLLAMSVVALAVSAFKMLHIGTLTR
                     GSGVAERALDHWVRDDTDAALALLGRTRGEAPALLRDGMQWLGAGKPAPSVREELVRR
                     ATRLLADAGNLLGVIEQVAYLAPLLGLLGTVLGIIDVFQGLAAHADASAGTLAGGIWE
                     ALLTTAVGLCVAVPFAIVHAALENRIERFRARMEDALTRLFTVTLYRAG"
     gene            30876..31277
                     /locus_tag="PLW13_00700"
     CDS             30876..31277
                     /locus_tag="PLW13_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY68915.1"
                     /translation="MLQSVRHAHSAKRVAMTPLIDIVFILLLFFILQSNFTQFRELLL
                     PVPATAEGAREGPRQLRIEVGADGRLWIDGVTLAAGGLGEFLDGRDLPGATPVVLVAA
                     PAVPVQLLVDLCDTLRSRKLQGIDIRALEAP"
     gene            31282..31686
                     /locus_tag="PLW13_00705"
     CDS             31282..31686
                     /locus_tag="PLW13_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY68916.1"
                     /translation="MIRLGRPARRAKDHDDKLVPMINVIFLLLMFFLIAGNLKPLFSE
                     EQLIPLSRSEALRAHARIELSLARDGVLTWGDEAMGAAQLGARLRALPDGVPAALSLR
                     ADARAQASALLPVLDAMRGAGVQRIAIVTLRR"
     gene            31692..32792
                     /locus_tag="PLW13_00710"
     CDS             31692..32792
                     /locus_tag="PLW13_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY68917.1"
                     /translation="MRPRRLLLWIALLAASLVLHFALSWQAGRELGLPGAGEPEQASV
                     AGIRLAPQPPEPEREELVFEAPPSMVQPAALLASTAAVTPPPNVNLALRAAAGAGSEM
                     PMPAPVQGFGLQQARGMGAAGFGTGIGNGLSNSSNRFAAYVQGLRDTGLDVVFVIDAT
                     GSMDWVLVEVRKRVADIADATRSLVPLTRFGVVAYRDRDDPGFVVREQPLTFSLAKLA
                     RFVDALRAEGGGSWQEAVDAGLEAAVNRAGWRPGAKRVVLLIGDAPPHEKDMERTLAL
                     ARELARAGGEISVLDVSNDANPALIEASLGRKVNRALYRERPMLQYESIAEAGGGTAA
                     TMDGDIRITRQLVSLIMGGQFSAEMSLLLEGL"
     gene            32792..34975
                     /locus_tag="PLW13_00715"
     CDS             32792..34975
                     /locus_tag="PLW13_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68918.1"
                     /translation="MRGAHSTAAALLALLIACVALLARGEDDAVHAFAAQLPEAAQWD
                     AARFSERADAFGKLVSAARDAMAGAPAQQRLRALGDELHAACRARIRALEAATGEDEA
                     KLEDLYRSEQWYAINYALAAVRYWQAWIDLALAGTAGDDAARLPALSRAERGFQSTAV
                     RILYPGLVYGSWLGMAYVERLRGDDAAAQSRLELLAGALAADPGNPLAKLVADELALL
                     AVRRGEAVSALPAGKGRLEPALARRLQEEAFALLEKRRSDGIAALEAAERLRRLVDAG
                     YLDDALLARMLAWRNELVGRDVGTIGLLLDAEYAAAHEQYETAVLKYREFAAKGGFEL
                     PIDSAPLRYNYAVALHRLGYEREALEVAMQLREDRAVGPAARKLVFLAAERINRDQPG
                     AANRAALSRAARDFLAASPDDKDAAFAHLALAGGGDAAARTHLAAARRDPRTRAAVEG
                     VRLDAAVVAFTAAAARNDLAARASAAREALAAIDALPRAERHALPLRVLALQMRVASG
                     DTGERTLRELQELQLAAGTDAALLRVLAWSRLRALSGDALSGYIGALGRPPSTADATE
                     LVAFLQEREQARDFATLQELAERALPAFASQPDVARQLVLMRHRALTALEREQEAFVV
                     AQEMVQAYPDSGDAWLAYAESAGRSGDTFAADRAWARIATATPEGSDRWLDATLRRVA
                     LLSRTAGAQAAHCDLLARAGLYRERMNAALGKQLAELERENRCDG"
     gene            35078..36457
                     /locus_tag="PLW13_00720"
     CDS             35078..36457
                     /locus_tag="PLW13_00720"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY68919.1"
                     /translation="MVRIRKYHADYSRRLFLQRLGQGMLATGVLMPLWQAIAQDGDSA
                     RAYPDEALSLEAYTKGKVKAGDVISAGNVEYVRDLLDPIQYLQVAQMGRRLDVLDTTT
                     DIMQLSPWEYVEATLRNKGRARFAPDGNVVTDEGRPWIGGNPFPQPGSALEVFAGITL
                     SWGRHDVSLYATKEEDLDDDGDVAYRYESVWTELSPVARVSLDPKPYWPGHEDKLRYQ
                     SILFLSPNDVKGTSFLNIWHYDQNRYPDLFGYLPAFKRVRRYPTNQRFEPLIPGSTLY
                     LSDAWAAGDPFLTWGNYRIVHQGPALGAVSRSWNATHPNWEHTTHGGANGLSFWDTAV
                     EFIPEAIVVEAEPVKYPRAPVGRKRVWFDARTLLPLSMVSFDRQGKMFRSFDGAFSLY
                     SDGSNAVMDGAHPYWSWTRVHAHDVQTNRITRLQQVRELPGGYRTTVNDQGVYEKYLT
                     QSALQRLGS"
     gene            36524..37546
                     /locus_tag="PLW13_00725"
     CDS             36524..37546
                     /locus_tag="PLW13_00725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY68920.1"
                     /translation="MTRKHKLWALMPALPKDLLVASVRQYEDAGLEGVWSPQLYGAPF
                     VPLAAAAAVSDRLKLGTGVALAFVRSPLETASCALDLDMISGGRAVLGIGPSIKWWNE
                     DWYGVRYGKPLAHLREVIGAVRQIIAKGHTGELGRIDGEYVKLDLERFKLLAPPLRTE
                     IPIYIPAVFEKTCEMAGEIADGLPGHPIWNAKWVRDKVAPHLANGLARSGRARRDFDL
                     NLFLFVAVNEDKRQAIEDARETIAFYGQMAQYEAYYEYIGFGSEARALQAAGARQDRA
                     ALMAACSDEMVESIVLAGTPEEVRRRLDAMSDIADSFTLSIPFYGLAPDKAAYYTQRI
                     AEVFYG"
     gene            37650..38465
                     /locus_tag="PLW13_00730"
     CDS             37650..38465
                     /locus_tag="PLW13_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY68921.1"
                     /translation="MEPLQAFSLAGKVALVTGAASGIGLECARVLGMAGARVMMGDID
                     HSGCAAAAAALRAAGLEVQARAQDVTAEGDWQASIEATRAAFGGFDVLVNNAGIYQGG
                     TLESNTLDEVRRVHRVNVDSVFLGMKFAAQAMKPGGAAGRGGSIVNLSSVAGLIGVPG
                     HTAYGSTKGAVRLYTKHAAVEFGALGYGIRVNSVHPGLIQTAMGEKVFEDFMALGLAP
                     TLDEAKAVVLGMTAVGRLGTSRDVANMVLFLASDASSYVTGAEFVVDGGMSAR"
     gene            38479..39249
                     /locus_tag="PLW13_00735"
     CDS             38479..39249
                     /locus_tag="PLW13_00735"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY68922.1"
                     /translation="MRMANKVALITGAGSGLGEAMARRLAQEGALVVASDIDFTAVQG
                     VVASIVAAGARAEAEHQDVTDEARWAALVDAILARHGRLDVLVNNAGIALTGSAEDTT
                     LADWQRTQRVNLDAVFLGTREAIRAMKTRGGSIVNISSIEGIVGEPLTAAYNASKGGV
                     RIFTKSAALHCAAEGYGIRVNSVHPGFILTPMVENALAGMPAEQAAAFHARIIDSIPV
                     RAMGEALDIANGVLFLASDESRYMTGSELVIDGGYTAR"
     gene            39255..39716
                     /locus_tag="PLW13_00740"
     CDS             39255..39716
                     /locus_tag="PLW13_00740"
                     /inference="COORDINATES: protein motif:HMM:NF023010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HQY68923.1"
                     /translation="MFAYTMEHICSYGVTLQSPFEVIGETPLGLRINAYVTGGTVAGP
                     RIRGEFLPVGGDWLTVRSDGVGVLDVRATIRTHDEALIYVQYQGVLELGNDGYARMLA
                     GNPPPCAQIRSAPRFLSAHPGYLWVNRLQCVNIGEVDFASASVSYDVYALG"
     gene            39842..41482
                     /gene="pgm"
                     /locus_tag="PLW13_00745"
     CDS             39842..41482
                     /gene="pgm"
                     /locus_tag="PLW13_00745"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HQY68924.1"
                     /translation="MPHPLAGQPAPKEYLTDIPALLAAYAGRQPDTGDAAQRVAFGTS
                     GHRGSALAGSFNEAHVLAIVQAVCDYRAQAGIAGPLYLGKDTHALSAPAERNALEVLA
                     ANGVDTRIQAGDGFTPTPVISRAILAWNRAASAPRADGIVITPSHNPPPDGGIKYNPP
                     HGGPADTDITGWIEARANALMAAGNRDVKRVPCAQAIAAASTTQEDFVGPYVEDLENV
                     IDMAAIRAAGVRIGVHPLGGAAVAYWQPISGRYGLDIEVVDPTVDPAFAFMTLDNDGR
                     IRMDCSSPHAMAGLVALKDRFDIAWGNDADVDRHGIVTPSAGLMNPNHYLAVAIDYLL
                     THRPQWSARAAVGKTLVSSALIDRVVAGLGRTMLEVPVGFKWFSGGLLEGSFCFGGEE
                     SAGASFLRRDGSVWTTDKDGIILGLLAAEITAVTGRDPGWHYRRLVAKYGAPCYARID
                     AAATPGEKAAFKRLTAERVAATTLAGDPITARLTHAPGNGAAIGGLKVVTANGWFAAR
                     PSGTEDVYKIYAESFVDPAHLQLILQEARRIVGEALRG"
     gene            complement(41517..42743)
                     /locus_tag="PLW13_00750"
     CDS             complement(41517..42743)
                     /locus_tag="PLW13_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68925.1"
                     /translation="MNPDLPSRRDDPVAVAFPELQNFSLVLGGPLWQLLRKARMDDDA
                     GSLVARRIVLASAIIWLPLLVLCAIEGTLLGGVDIPFLPDIETHARFLVGVPLMILAE
                     LVLHQRLRGIVAQFVERRLVPAASMERFIEALRSAMAWRNSWRVELALIALVYTVGHY
                     IRDYIELDTSSWYATVGTGTGTGTGTLTAAGLWFTFVSNPVLQFILLRWGYRLLIWAH
                     FLWQVSRIELDLIPTHPDRNGGLGFLGGSAYAFAPLLSALGAGVAGMIANRIFHQGAS
                     LAAFKLEIVLLVTMGVVLLMAPLLVFAPKILAAQRQGLREYGAFTAEYMREFDRRWLR
                     SGDHDREALLGTGDIQSLADIGNAFTVIREMKPLIFSRTMLVQLTAAMLIPFVPLVFT
                     MIPLEQLLDRIIGAIF"
     gene            complement(42808..43806)
                     /locus_tag="PLW13_00755"
     CDS             complement(42808..43806)
                     /locus_tag="PLW13_00755"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HQY68926.1"
                     /translation="MRRAWRAAGPGRLRVLMLALALLAGCSLNPPLELDTIAPAATPV
                     RLSSVPFFAQTEFQCGPAALAGVLGAAGVATDPATLAPQVYLPARQGSLQLELVAATR
                     RAGRLPYVLAGEPRALLGQLQAGRPVLVLQNLGTPQVPVWHYAVLTGFDAARNRLYLN
                     SGSERELPVHAPQFLRTWDWAGRWAMVALKPGELPAGADPGRYLETATAFERVADRDA
                     ATRAWQAAADAWPEQPLPPLALGNLAYRDKAPARAAAHYRDGLARNPDDPALANNLAS
                     VLGALGCPRRAEALLQPVARRLADDSPWKATIEATLRELGALTGMDELSCDAMPSP"
     gene            complement(43810..44181)
                     /locus_tag="PLW13_00760"
     CDS             complement(43810..44181)
                     /locus_tag="PLW13_00760"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HQY68927.1"
                     /translation="MRKKLVAIALSGSLVCAGFSTGVAAAVIDTQQAVTMDAREQRIA
                     AVSATLAREDVRSAMIELGVEPQQAQLRVASLSDAELARLEGQLQQLPAGGSALGLVG
                     AVFLVLLILEITGVIDIFKKL"
     gene            complement(44343..44972)
                     /locus_tag="PLW13_00765"
     CDS             complement(44343..44972)
                     /locus_tag="PLW13_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQY68928.1"
                     /translation="MNRPPGERRANQRLRTRKDLLVAAARLLEDGRTPGMDEIAAAAM
                     VSRATAYRYFPTLEALLLEAPLQGETPDPAALFAGDDSREAEARIDRAEAALHRMSYR
                     NARQLRLMLAHSLAREPAEAVPRRQNRRTPLIEAALEPARAELDPAAFRRLCRALALV
                     FGTESMIVCSDILQIDEQAARELKSWTVRALVRAAMQEARPAVARRKQA"
     gene            complement(44979..45401)
                     /locus_tag="PLW13_00770"
     CDS             complement(44979..45401)
                     /locus_tag="PLW13_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQY68929.1"
                     /translation="MTLTTLQMDRAIDEHFGYEAADDIDGVVATLAPDVIHDIVGWPG
                     GPTFGPDNARPFYERLFADLADGQVQCVKRLYGENFLVDESLWRGTAAGRPFGVEGHG
                     RALEFRLLHLIEFTPAGRIGRENVWVDLAAIQQQLAPV"
     gene            45669..47822
                     /locus_tag="PLW13_00775"
     CDS             45669..47822
                     /locus_tag="PLW13_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY68930.1"
                     /translation="MNTRKAGLLSLIVLLLALLFALDLDRLLTLEGIKASRAGLALLL
                     ERSWPLVAAGYFLLYVLIAAVSFPGAAVMSLAGGALFGLAWGTLLVSFASSIGGTLAF
                     LVARYLLRDAVQRRFAGWLRAVDEGFARDGAFYLFTLRLLPLPFFLVNLVMGLTPIRV
                     RTFYWVSQLAMLGATLVYVNAGTQLARVEALSDIVSPSLLLSFALLGVFPLIARRVLA
                     ALQARRAYAKWQRPRRFERNLVVIGAGAAGLVSAYIGATVKASVTLIEARRMGGDCLN
                     TGCVPSKALIRSARLAHEMRHAGRFGLEALEPRVEFRRVIARVQEVVRAIAPHDSVER
                     YSALGVEVLEGHARIVDPWTVEVHSGDGRTRRLTTRAIVIAAGAEPYVPPLPGLDEVG
                     YLTSDTLWDALAAMERPPQRLVVLGGGPIGCELAQCLQRLGSQVTQVELMERLLARED
                     PEVAHGVQDALARDGVEVLCGWRAVHCERVGEHRFLVVEREGVQRRLGFDALLCAVGR
                     KARLGGYGLEELGVPVAGTVVVNEYLETLYPNIYAAGDVAGPFQFTHAAAHQAWYAAV
                     NALFGAAWKFRADYRVIPATTFTDPEVARVGLNEQDAQARGIAYEVTRYGIDDLDRAI
                     ADGAAEGFVKVLTVPGKDRILGATIVGAHAGELLAEFVLAMRHGLGLNKILGTIHSYP
                     TLAEANKYAAGEWKRAHAPGRLLRWVERYHAWRRG"
BASE COUNT         7456 a        15848 c        17004 g         7563 t
ORIGIN      
        1 ctagtggaag agctgcgtcg acccggcccc attgggcgag gcggaatccg gcgaagcgct
       61 gctgcgcccc gccgagcgcc cgaaatctgt gatttcgggc cgggcccgaa gggtattcac
      121 ggcgagtccg aacgggtgac tcgccgcccc ggtgcgctgg cactgcgcag ggcaacgaat
      181 ggtttccgga tttacgcagc aggagcagga caatggattt cggattctcg gaagaacaac
      241 gcgacataca gaacctggcg cggcagatcc tgaaggacct ggtgtcgccg acgacgctgg
      301 cggcgtatga cggctacgcg agcgagcgtt tcgaccgggc gctgtggggc aagctggccg
      361 aggccgggct gctcggcgtg gcggtgccgg aggagcatgg cggcatgggc ttcggtttca
      421 tggaggtcgc gctgctgatg gaagaggccg ggcgcagcat cgcgccggcg ccgctggccg
      481 tgcacatggc cggcggcatc gtgccgatcg cgcgcttcgg cagcgcggaa cagaaggccc
      541 ggttgctgcc gggcgcggcg agcggcgaga tcgtgctcac ggcggcgctg gaagaacagt
      601 tcgcgctgga tgcctgcgag cccgccacca cggcgcgcgc cgagggcggc ggctaccgcg
      661 tgagcggcga aaagttcgcg gtgcccttcg cgcactgcgc gcagcgcgtg ctggtgggcg
      721 ccatgagcga tgccggtctc gtggtgctgc tgctcgaccc gcgggccgcc ggcgtcacgc
      781 tcaccgccat gaagacctcg agctacgagc cgcagtcgct gctcgtgctg gacaacgtgc
      841 tggtcccggc cgccgacgtg ctggccggcc cgcagcacgg cgccgaggtg atgcgcatcg
      901 tgaccgagca cctgacggtg gcgctgtgcg cgcaccagtt gggagtcacc gacacgctgc
      961 tgcgccagac cgcggcctac acctccgagc gcaagcagtt cggggtgccg atcgcgacgt
     1021 tccaggccgt gggcaatcgc gcggcggatc gcttcatcga cgtcgagtgc ctgcgcctca
     1081 ccacgtacca ggccgcctcg ctgctggcct cgggtgcccc ggccagcgtg gaagtggaca
     1141 tcgccaagat ctgggccggc gacgtgggcc accgcgcgag ctacacggcg cagcacctgc
     1201 atggcggcgc gggcgtggat cgtggcaatg cgccgtggcg ctactgcctg tgggcgcgcc
     1261 acaacgaact gacgctgggt tcgaccgcgt cgcgcctggc cagcctgggg cagcgctgcg
     1321 cgaacggtga ggcggtggtc gactgagtac gacgatggac gtggaacgcg gccgggtggc
     1381 gcggatcaca gaccgaagca gttcgcgtaa taggtcacgg tccccggcca gtcgctgaac
     1441 acgcccagca cacccacctg gcggccgagg acgtccagcg cctccatcgc gacgccttcg
     1501 ttcccgcgct gtgccagcac atcactgatg ttctggtagt agaagccgcc gttgccgccg
     1561 gcgaggaggc cggagcgctc gagggtccag gtgatcatgt ccaggccgac cgacctggcg
     1621 tcgcgagcgg cagcggaagg caccagtgcg ccgttctcgc tggagatcag cgcccacatc
     1681 ggcggcgccc agatcctcac gccctggttc gccagctgcg cgagttccgc ccggtccggc
     1741 agctcatcgg tgacgttggc gtcgtcgagg aacacggcct gcctgccgaa gtccggttca
     1801 gaccggatcc agtgcagcac gtcgtcgagg tcgaaggatt gcggccacac cgcggcgggg
     1861 cgcacgccgg attgcctgta ctcgtcgatc atcttctgcg catagtcgcg ctggctgaat
     1921 ccgtcgaagg gcatcgtcac gctggggctc ttgagctcgg gcgtcatctt caccccgagt
     1981 ttcctgaaca acgcgatgct ctcgcggtgg gtcatcagcg tgccgctgga ggcgccggca
     2041 tagagatcgg tgcgccagtt tggcgtaccg gcgacgtatt cctgcacggt tcgtgcgtcg
     2101 ggatcgaacg catccatctt gcccttgagg gtcctgaact cggcgagcgt gatgtcgctc
     2161 gtccggcatt cggcattcgc cgccgtcaaa cgattgccct cggcatcgaa agtcgccggc
     2221 gtgaacggcg ttgtgcactt caccgccagc tccgggacca gcaggatgtt ggtcgtggtg
     2281 tgcaggtcgt tctgtgaatg acggcacacc agttccttgt ccctggtgaa ggtgacgtcg
     2341 cactcgacga tgcctgcacc cattcgtgcc gcggcctcgt aggactcgcg cgtgtgctcg
     2401 gggaactgca gcggggcgcc gcggtgtccg atcgagaaat ccgtcttcct gaacggcttg
     2461 ttcctgcagt cctgcgagcc gagccgctgc ttgagcggac tgtcgtccat gtcggcgacc
     2521 aggaaatagg ggcgtggacc gacctggata ttgagtactt ccggggccgc gagcgcgggc
     2581 atggcgagca gtgcgccgag aacaagtgtc gcgagcttgt gcatttgccg aactttctcg
     2641 cggagctcgc gggcagcggc gcgcatttcg tgtcgccgtg ccgagcgtcc gctcgatggc
     2701 atcagcgctt cacgtgctcg atatccccct ggtccaccag gttccattcg ccggcgttgc
     2761 ggaacaggcg cttctccagg cgcgggtagt cgaccacgtc gtgcgccagg cgctggccca
     2821 cgaccaggca gaccagcggc tcggcgccgt cgttgatcag ctcgtgcgcg atgccgttcg
     2881 ccgggcagcc gatgaagtcg cccgggccga tcggctgcag ctgctcgccc agcaccgcca
     2941 gcccgcgccc cgagaggacg tagatgcact cttcctcgaa gtggtgcacg tggtactccg
     3001 tcgactggtg tcccgggtcg accgtgatca ggtgtgcgcc gaggtgctgc atcccgaccg
     3061 cgtctcccag tgacttgttc aaccgctcgg cggcgggatt caggtagtgc accttgcgca
     3121 cgccttccat ttcctcgatc tcacgggcag acaggtacat cgttcgattc tccggtgctg
     3181 atgccggcca gtatactgtc cggcgccggg gcttcggccc ggcaggaatc acgatattgc
     3241 cacggacgtg ttaccgatgt gcgggcgcta caacatccat gacgatccct ggctgcagac
     3301 gctgctgcgc gagctcggcg tggagatgcg catccccacc cgcgtgaaca tcgcgcccac
     3361 cgaggcggtg cccgcggtga tcgaggagca gggcaggcgc tgcctgcgcg agatgcgctg
     3421 gtggctggtc ccctcgtggg cgccggagat cgaccagcgc tactcgatgt tcaacgcgaa
     3481 ggccgagacg ctcggcacca gccgtgcctt ccgcggtccg ctgcgccacc gtcgctgcat
     3541 cctgccggcg tcgtcgttca tcgagtggac cgacgccggc ggtcacaggc agccatggct
     3601 catccgtccc gagcgcggcg cgatcgcttt cgccgggctg tgggacgtgt gggagaagga
     3661 gggcaatcac ctcgagtcgt gtgccatcat caccaccgat gcagcccccg gcatcgaccg
     3721 cctgcacaac cgcatgccgg tgatgctgcg cgagcgggat ttcgcgcgct ggctggacgt
     3781 cgacacgccg ctcggagatc cctcggatat cctgcattcg cgcctgcctc atccgtttgt
     3841 gcttgcgccg gtctcgccgg ccgtaaacaa ttcgcgccac aagagcgagg cgctgctcgc
     3901 gccggttggc gaggaggagc gggtcgaggc ggtggggcag tgggcgaaat gagggaaatg
     3961 ggagaaatga gcgatgaccg attccctgct cccggagcag cggcactggc tgccgacggc
     4021 agcctgctgc tgggtgcgcg gcgcattccc ctgccggcct cggtgagccc ggaggcgcgt
     4081 gccttcctgg cgatgccgcg ggtgacgctc ggcgagcgtc cgccgctggc ggacaaggag
     4141 gcgtggcggc gctgggtcac tacgatgaac ctcggcagcg agccgcgtgt ggcgcagatg
     4201 atggcgctgg taggggatcg cgtgcgcgtc gagacgcgca ccatcgccgg cgtggtcgtg
     4261 catgtcgcca ctccagccga atcacacccg tcgcgccgag actggctgcg catcacggtc
     4321 catggtggcg ggctggtcta catgggcggc agctatgcgc gcgccgaggc gggcctggtt
     4381 gcgttgcagt cgggttgcga ggcctggtcg gtggattacc gcatgccgcc ggaccatccc
     4441 tatccggccg cggtggacga cgtggttgcc gcgtatcgcg ccgcgctcgc gacgcgtgcg
     4501 ccgggatcgg tcgcgatttc gggggcttcc gccggcggca atatcgcggc ggccgcggcg
     4561 ctgaaggctc gtgacctggg cctggcgttg cccggtgcgc tcggcctgtt cacgcccgag
     4621 tgcgatctca cggaatcggg cgacagcttt gtcacgaacc tcgacgtcga caacgtgctg
     4681 cccggaccgc tgcccgaaga gatcgcgctc tacgccgacg gcgcggacct gcggcaccct
     4741 tacctgtcgc cgctgttcgg cgatttcgcg cccggctatc cgccgacgca gatccagacc
     4801 ggcacgcgcg acctgttcct gtcgaactcg gtgcgcatgc atcgcgtgct gcgcgccgcc
     4861 ggcgtgagcg cggaactgca cgtgtgggag gcgatgccgc acggcggctt cggattcgat
     4921 gcgccggaaa acgccgatgc gcgacgcgag ttcaacgact ttctcgaacg acacctcgga
     4981 taacacaagg agcgcgcacg cgtggcaaca cagatgggaa actcgacgga aatgcacgcg
     5041 gagttgctgg gcctgccgcc ctgcgaccag gtcggcttcg tggtgcgcga cctgcaggcc
     5101 gcgatggcga tgtacgcacc gctgttcggg ccgttcagca ccatggacgg ctcggtgaag
     5161 caggccacct accgtggccg ccttgccgac gccgggctgc agatcgcctt cgggcgcagc
     5221 ggcaatctcg agatcgagct gatcgagtgg acgggcggcg agagcccgca ccgcgagttc
     5281 atcgaggccg ggcgcgaggg catgcatcac atccgtttcc gcgtcgagga caccgaccac
     5341 tgggtcgagc gcctgcgccc gctcggttat gaggccgtgt ggtacaagcg ctggtcggcc
     5401 gacaccgtgt tcgcgtacct ggagcgcccg ggcgatccca cgctgatcga gttcctgcag
     5461 atgccccccg gcggtccggg agcctgatcg tccgatcgcg gggagcgctg cacatgatcg
     5521 agctcgcgcc gggcgtcgcg gtggccgacg aggagatcga gatcaacgcg atccgcgccc
     5581 agggtgccgg cgggcagaac gtgaacaagg tctccaacgc cgtgcacctg cgcttcgacc
     5641 tcggcgcctc gtcgctcgac gttgaactgc gcgaggcgct gctcgcgctg cacgaccagc
     5701 gcatcagcaa ggatggcgtg atcgtgatca aggcccagcg tttccgcagt ctcgagaaga
     5761 atcgcgacga tgcgctgcag cgcctgcgcg agctggtggc gcgcgggctg ctgcgccgcg
     5821 cgccgcgcat cgccacgcgg cccagccgga gcgcgcagcg caagcgcgtc gacgaaaaga
     5881 gccggcgcgg gcagatcaag gccgcgcgcg ccccggtgcg ccgcgacgac tgaggagcct
     5941 ctcgatggac cccgtgatat tcctttggct gattgccatc gcgctggtgg cggccggcgt
     6001 ggccgggctg gtcctgccgg tgctgccggg cgctgcgctg ctgctggccg gactggtgat
     6061 ggcggcgtgg atcgaggagt tccagtacgt cggggtcggc acgctggcgg cgctggccgt
     6121 cctcggctgc ctgatcttcg cggtggactt cgtcgcgggc tcgttcgggg cgcgccgctt
     6181 cggagcctcg ccgcgtgcgg cgctcggcgc ggcgctcggc gcgcttcttg gtctattctt
     6241 cgggttgccg gggctgctgc tcggtccgat ggccggcgcg gtgctcggcg agatcagcgc
     6301 gcggcgcacc tggtccgagg cgggacgcgc cggtgtgggc gccacgctcg gcctggtgct
     6361 gggtgcggcg gccaagctgg ggctggcgct cacgatgatc ggtatctttc tgttcgtgcg
     6421 tttcctggga tgacaatgac gcggattgcc ttgctcgtga atctcctgct ggtgtcctgg
     6481 ctgacgggct gcggctggat ggacgggccg gagcccgggc cggcggcgcc gccgccggat
     6541 cagcgccacg gcacgctgcc cgggcaggtg tcgctcgcgg accccgaggc cgcgcgggcg
     6601 gcgctgctgg agcagtacct cgactgggcc ggcgtgccct accgcatggg cggcagctcg
     6661 cgcgagggcc tggactgctc cggcttcatc cagctcacct acctgcagcg cttcggcgtc
     6721 gagctgccgc gcgatacctc ggcgcagcgc ggggtgggca gcaaggtggc gccgtcgcgc
     6781 ctgctcccgg gcgacctggt gttcttcgat accgggcccg ccgagcgcca cgtcggcatc
     6841 tacgtgggca agcgccagtt cctgcacgtt tcgagcagca ccggcgtgat gctgtcgcgg
     6901 ctggacgacg gctactgggg ggcgcgtttc cgcgacgcga tccgcgtggc cgagcgttga
     6961 tgtgggttga tttatggcaa cccgcatgaa ccgccgggcc ttgtgccttg ccatcgatgg
     7021 gcgaatactc tcgagtacgc acgcggagag cggttgtacc gcgcgcgccg gtcgatcaca
     7081 cgatgccctg cgagcggagg tggatcatgt ccgagcgaag actttcgaat cgccagccga
     7141 gcctggtgtt ctacgacctg agcttcgccg acagcgggga gcgcttcggc gagatgctcg
     7201 atctctccag tgacgggctg atggccctgt gcgatgcgcc ggtgctgccg ggcagccgtg
     7261 tcgcgctgca gctggacatc gcggcgcgca tgctcggtac gcgttcgctc agtttcgcgg
     7321 ccgaatgccg ctggtgcgcg ccgggtgcga cagcgggtgg ctatgcctgc ggcctgcgcg
     7381 tgctggcgca ggacgatgcg tcctcgcgcc agctgcccca cctcgccgcg accatggtct
     7441 tcgattcgct gcgggcgttc tgatcagcgg ccctggacgg ccttgcgcgg cttgagcgcc
     7501 agcgtgggta tcagcgtcag caggaaggcg atcgcgagga tcacgaagct gtcgcggaat
     7561 gccgcggcga gcgcctgctg ctgcaccatg ccctgcagga aggcgcgcgc ggcatgccat
     7621 tcctcggtgc cggcgaatcc cagcgtatgc gacatgccgc gcagcaggcc cagcatgttc
     7681 aacgtctcgc tgctgccgta ggtctgcgtc gacagcaggt agtcggcgtg gtagatgtgg
     7741 cgccgctcga gcaccaccga catcaggttc acgccgaatg cgccgccgag ctggcgcatg
     7801 aagttgaacg cggccgaggc ctggctcagc cggctgatct cgatgtggct gagcgcgccg
     7861 agctgcaacg ccggtatcac cagcccgatc cccaggcggc tcagcacgag ccaccaggcg
     7921 agcgcccaga agccggtatt ggcgtcggcg ccggacatga gccagaacga cgtcgcgaag
     7981 aaggcgatgc cgccgctgat cagcaggcgg tggtcgagca cctcggcgag cttgccggca
     8041 aacggaaaga gcagtgccat tgcgatgccg ggcggaatca tcagcgcgcc cgcccgggtc
     8101 gggctcaggt gttgcaccag ttgcaggaac accggcacca ggtaggtcga ggcgaagagg
     8161 ccggcgccga agacgaagct gttggtcgtc atgatcgtga agttgcggtt cgcgaacagc
     8221 ccgaggtcga gcagcgggtg ctcggtgcgc agctgccacc acacgaagcc cgcgccggac
     8281 accagcgcga cggcgaagca gccgaggatg tagtccgatc cccaaccctc cttctcgccg
     8341 ttgctgagtc cgacgagcag cgcgatcagc gccacgctga gcgcgatcat gccgctccag
     8401 tcgaagggga cgcgggtggg caatggctgg cgggtgggca tgaagaacga ggaagcggcc
     8461 agcgtcagca gcgagaacgg gatgccggca tagaacacgt agcgccagct gtacgtatcg
     8521 atcagcacgc cgccgatcgc cgggcccatc gccggcgcga ggatggcgcc gaccgaggtg
     8581 atgccgatcg caacaccctg cctgccgggc aggaacacct ggaagatgag catcgtggac
     8641 accggggtga gcacgccggc ggtgatgccc tgcatgatgc gggcgacgat catgatcccg
     8701 gtgttggtgg ccagcccgcc gagcagcgag ccggcgagca gcaggtaggt cgtgatgtgc
     8761 acggtctggc gcagccccag cacgcgcacc atccacgagc tcgcgagcat cgctaccgtt
     8821 gccgcggcca ggttcgccgt cgacagccac tgggcgtcga cctggctgat gccgaaggct
     8881 cccatgatgt cgggaatcgc gacgttgacg atcgtaccgg cgagcagcgc ggcgatgttg
     8941 ccggcgacga tcgtgagcag cgcgaaccag cggtacttga ggccccaggc gcgaaagccc
     9001 gtagcgaagc ccgtgttcgg cggcagccgc gcgatgtagc tctctacctc gtcgtcagcg
     9061 gatgtcaatc gcgacctcga ccatcatgcc cgggcgcagc agctcgtccg ccggttcgat
     9121 cgtgatcttc accggcaggc gctgcgtgac cttggtgaaa ttaccgctcg ggttggtatt
     9181 cggcagcagc gagaactggc tggtggcgga ctgccccacg cgcaccactt cgccgcggaa
     9241 ctcgcggtcg gggtaggcat cgacgctgat cgtgacctgg tcgcccggct cgacatggcg
     9301 gatctcggtc tcccggatgt tcgcatcgac ccagatgcgc tggggatcgt gcatcagcag
     9361 cagccgctgc ccgggcacca cgtactcgcc cacgtgcacg aaggtctcgt cgacggtgcc
     9421 cttgccggcg ctggtgatgc gcaagtcgcc gatcatcacc ttctgcttgt cgatcgaggc
     9481 gcggatgcgg tcgcgttcgt gctgcagccc ccgcagttcg ttcgcgagca cctggatccg
     9541 gctctgcccg gcctcggctt ccacgacgtt gccgcgggcg ctctcgactt cggccagcgc
     9601 acgctggtgg agctgctcgg actgctggtg cgcgttgcgg gcgttctccc actgctgctg
     9661 cgagatgatc tggcgttccc gcagtgactg cgcgcgctcc cattcgccct gcttgaactg
     9721 caggtcggac ctggcggagg cgagcgaggc ctgcacggcg tcgaggtgcg acttggccga
     9781 gctcagctgg ccaccggact cgcgatcgac catgtcgatc tgcacctcga ggcgctggca
     9841 ggccgattcc atggactgga actgggcctc gagctcggcg agcgtgaggc gcgcggcgcg
     9901 atcgtcgatc tgtgcgatca gcgcgccggg cagcagttca tcgccctgcg agacggggaa
     9961 ttccacgacc tgcccggcga ccttggcgct cacctcgatc atgctggtgg cgatgcgcgc
    10021 gtcctcgctg aagacgtgcg tgtaccgctg gtacagccac caggcagtgg cgatacccag
    10081 tgccacggtc gcggcgacca gcagcgcgcg gcggcgtgcg cgctggttca tcacgagtcg
    10141 atgctcggcg gattgcgctt ggttgtctgc ggacatcagt gtgctccggc aaatcgggat
    10201 tctgtggcct ggggggattc ctcgagcagg cgctggcgga tccagccgag gctgttcacg
    10261 agcagttgcg tgcgccgagg cccgagcggc tgcagcagcg cggcatgcag ttgctcgaag
    10321 cgctggcgct tgccgctgag caggtgatgg gcgcggcggg tcaggaacag ccgtttggca
    10381 cgccggtcgc gcggatcggc gcgccgttcc agccagccgt ccgcctgcag ggcgtcgatc
    10441 accttgccga gcggcgccgc cccgatctcg gtggcatccg ctatcgcggt ctggcgcatc
    10501 ccttcgttgc ggtacaccgt gttcatgacc agccattgat tgcgcgtgaa gcccatttcc
    10561 gcgagccggg catcgaagcg ccgggtcagc aggcgcgcgc actcgaacag cacgctcacg
    10621 taggtttcgg tcgtggcgtt cgtggtggtg gtgccggcag tgccggtagt ggcagtggtt
    10681 ccggtgacag tgtcggcggc cggtgcggca atagctacgg tccgcgcggc ggaggcggac
    10741 gtcggcgctg cggcgcgcag cgcgtcacgg atgaccagga ggctttccag catctgcgcg
    10801 acctcctcgg catcaaagcc gcgcaggtag ttggcctcga tggcacggaa tcgttcggac
    10861 atccgcgcca gggccggttc ggccctgggc agcagcgcgg cgcggcgtac cctgcggtcg
    10921 gccgggtcgg ccttgcgctc gatccagccg gcctgttcca gccagtccag cgcgagcccc
    10981 agcggcgcct tttcgatgtc cagccgctcg gccagctccg actggctgat gccggggcgt
    11041 tccatcaggg tggtgagtac ccgccatttt gcctgggtca gttggagatc gcgtatcgac
    11101 agcccgaaac ggcgccggat caggcgcgcc gtgtcgtgga tgaggaaggc gcagttctgt
    11161 tcgaaatccg gaagcgacat gggatgacgt aaatagtaat gcacatataa tatattcctg
    11221 cgtgactata tgatccagtt acttgttggc aaggccgcga ccggaggccc agggtgcccc
    11281 gagatgcccg gtgagcagga cctcagcgtc tggacaggtc gatcactccc gggggccggc
    11341 attcgttgat gaactggccg aattcacgga actccgccgc tcgcgcgctg ccctcgcgcc
    11401 acaccagacc gatgtcacgg taggacttgt tgtcgagcgc atgggtctcg ataccggtgc
    11461 cctccagcag cgacgagcct tcggccatgg cgggcaggta ggtgacgccg aggtccgccg
    11521 ccaccatctg caccagggta tagaggctgc ttgccgcgaa gcggttcagc ttgtcggggc
    11581 ggcgcagccg gcaggccgag agcgcgtgat cgcgcaggca gtgcccgtct tccagcagca
    11641 gcacgctctc gggcgctagc tgatcgacgc gcagccgccc cttttcgagc aggggcgagc
    11701 cggcgcggca ggcaagccgg aagggatcgc ggaacagcgg caggacctcc gcgttgcgga
    11761 tgtcccaggg cagagccagc aacagcaggt cgagctggcc gtcgaacagc gaggccagca
    11821 gccgctcggt cgtttcctcg cgcaggtaca acttcatgtg cgcaaagcgc gcgctcgcgg
    11881 ccggcatcac gcgcggcagc aggaagggcg cgatggtcgg gatgacaccg aggttgatgc
    11941 gccgcgccag cggcgagcgc tcgcgttgcg cgaattccac cagttcctcg gcgtcggcga
    12001 gcgtgtgcct ggcgagcacg gccacttcct gccccaccgc cgtgatcgtg acctgtttcg
    12061 atgtgcggtc gaccaggctc agctcgagct gagtctcgag ctcgcggatc gcgatcgaga
    12121 acgccgactg cgacacgtgg cactccgcgg cggcgcggcc gaagtgacga tgacggacca
    12181 gcgcgacgaa atagcgcagc tggcgcagcg tgggcatgtg gctcatgcgt ctctcctcat
    12241 cattcagaaa acagattgaa tcactaaatt caatcaattt cacaatttat tttgccggcg
    12301 ccagaatggc gcccgtgtca aacagcctgc gatgcgggca tggaggaacg caaatgaaac
    12361 agtccggaat tcgcgacatc gacatcggca ttccgcgcga gcaacgcgct gcgatcgcgc
    12421 agggcctgtc gcgcctgctc gcagatacct acacgctcta cctcaagacg cacaactatc
    12481 actggaacgt cacgggaccg atgttcaaca cgctgcacca gatgttcgag ctgcaataca
    12541 acgagcttgc gctagcggtg gaccagatcg ccgagcgcat ccgcgcgctg gggttccgcg
    12601 cgcccgggtc ctaccgggaa tacgccgcac tcacggtgat cacggaggaa gacggcagtc
    12661 ccgatgccat cggcatgatc gagcgcctgg tcgatggcca ggaagcggtg atccgcacgg
    12721 cgcgcagcct gatcccggag gcggccgagg cgcacgacga gccctccgcg gacctgctga
    12781 cgcagcgcct gcaggtgcat gagaagaacg cctggatgct gcgcagcatg ctggactgat
    12841 cgacgccccc gttgcggcgg gggcacgatc cacttgccgc gcgcgcggtg gcacaatggc
    12901 gtcaccaacg tgtgtgcgtc aggggcggct atggacgtca gcggattgac agagcccggc
    12961 ttcgagcgcg tgctcgaggc cttcgcgcac aacttcacgg ctcacggcga catcggcgcg
    13021 gcatgctgcg tctacctgca tggacgtccg gtggtcgaca tctggggtgg aaccgccgat
    13081 ccggtggcgg gcataccgtg gcggcaggac acggtgcagc tggtgttctc ggctgccaag
    13141 ggcccgaccg cggtctgcat ccaccgcctg gccgaacagg gcctgctcga catcgatgcg
    13201 ccggtggcgc gctactggcc ggaattcgct gcggcgggca aggagcgcat caccacgcgc
    13261 atggtgctct cgcaccgggc gggtctggcc gcggtcgcgg gcgatctcgc gctggccgat
    13321 gtgctggcct gggatccggt ggtcgaggcg atcgcggcgc aggcgcccga gtgggagccc
    13381 ggcagcgcgc acggctacca cgcccgcagc ttcggctgga tcctcggcga gatcatccgg
    13441 cgcatcaccg gcgagagcgc gggacagcat ctggcccgca cggtctgcgc gccgctgggc
    13501 cttgactacc gggtcggcct gccggccacg gaacgcgcgc gctgcgcgcg cctggtgccg
    13561 cccgatgccg accattccgc cgcggcggcg ctcggcgcca acagcctcac ggtgcgcgtc
    13621 atgaccgggc cctcgggact gttcggctac aacgagatgt ggaaccgcga cgacgttctc
    13681 gccgccgaga tgccctcgtc caacggcgtg ggctcggcgc gcgcgctcgc gcggctctac
    13741 gcggcgacga tcggagaggt cgacggcatc cggctgctgc gacccgggac ggtgcgcgag
    13801 gcgacgcgcg tggtctcgcg gggtcccgac agggtcattc tcgtcgagac ctgtttcggc
    13861 ctgggattca gcctgccgcc gatgctcggg cccgactgcg ggccgaacgc gttcgggcac
    13921 ccgggcgccg gaggctcgct gggctttgcc gatccggagg cagggctggc gttcgcctac
    13981 gtgatgaacc gcatgcgctt cgaggcaggc ggtgatccgc gcgccgcctc gctggtgcgc
    14041 gcgctgtacg agtgcattgg ctgagacccg ccgcgcgggc gttgatacgg agaccccgga
    14101 tggaactgac gatactgagc gatttcgaga tgccgggact tgccgatgcc atcgcgcgcc
    14161 agggcgccgg agcccgcctg ctcggcgtgg cggcggacga gccgctgccg gcgcacctgc
    14221 cgggcgcggc gctgctgacg acggcgctgg gcaacccccg gctggaggcg ctgctcgccg
    14281 cggcaagcgg gttgcgctgg ctgcacgtgt tcggcaccgg ggtggacagc ttcccgctgc
    14341 atctggtgcc gccgcaggtc acgatcacct gctcgcgcgg tgccagcgcc gtgcccatcg
    14401 cggagtgggt gctggcgatg atgctggcgt tcgaaaagca gttgccgcag tcctggatca
    14461 gcgcggcccc ggcgcggatg tggcaggcga acctcggcac gctggccggc aggacgctgg
    14521 gccttgccgg ctgcggtggc atcggcgcgg cggtggcttc gcgcgcggcg gccttcggca
    14581 tgcaggtggt agcgctggcg cgctcgccgc gctccgagcc accgcccggc gtcgaaatgc
    14641 tgcgcacgaa ggaggaactg tgcgagcgcg ccgatcacct cgtgctgtgc ctgccgtcca
    14701 cgccggcgac gcggcatttc atcgatgccg cggcgctggc gcgcatgaag cccggcgtgc
    14761 acctcgtgaa cgtggcgcgc ggcgcgctgg tggaccagga ggcgctgcgc gtggcgctcg
    14821 acgcggggcg ggtcgcctgc gcctcgctcg atgtggccga acccgagccc ctgcccgagg
    14881 ggcactggct ctacacgcac ccgcgtgtgc ggctgagccc ccacatctcg tggagcgcac
    14941 cgcaggcctt cgcggcgctg ctggcgggct tcctcgacaa cgtgggacgc ttcgcgcgcg
    15001 ccgaggcgct cagcggtatc gtcgaccgcg aggcgggtta ctgagcccgc cgcttcctca
    15061 gcggcgcgta tcccagtagt tgccttccat catcgcctcg atcgcggggc ggtgcgggat
    15121 catgtcgtcg ggattctcgg ggatcgtgct ctcgccgaaa agcgcctggc gccggccgat
    15181 gcggaacatc acgatgccgt gacgcagcgc cgaatagaac gtgtagaact ccatgtcgcg
    15241 cggctggtgc ccggtggcgg cggcataggc ggccgacatc gcatcgaggc gcatcatgtc
    15301 ggggaggccg ggcagcccga ccagcggcgt gaagtcctgg aagaagtggt ggatgtagat
    15361 catccagccg agatcgatct cgcgcggccc ggtggcgacc atctcccagt cgagcacggc
    15421 gctgggctgg aaatccacga acagcatgtt gccgatgcgc gagtcgcccc agctcagcac
    15481 ttccgggcct tcgtccttcg gccagttggc ctccagccac gcgaaaccgc gctcgatcag
    15541 gggcgagcgg atgccgtcgc ccgccaccac ccagtcgtaa tagctgcgct gcgaggcgac
    15601 gtgacgacgt agcgccgttg ccccagcttc gcgcgggcgc agcagctcga gatccgcggg
    15661 gccgagctcg atggcatgca gcctggcgag cacgtccacc gcgcagcgct gcagccgctc
    15721 gcgttcgccc gggctggcgc gcagcagcca ggagtcgaac acgtagggcg gcacgtcggg
    15781 cggcgcgtcg ccgtcggcgc gctccatcac gaagaacggc gccccgaggt gcgaggcgtc
    15841 ggcctcgtac cacagcatgc gcggcaccgg cgcgctggaa cgctcgcgcg cgagttgcat
    15901 cacgcgaaac tgcttcaaca ggtcgtattc ggggaaaacc ggcatcgcgc ccgcatccgg
    15961 cggcaggcgc gcgacgcagc gaagcgaacg ctcgacaccg tcctcgcgca actgcaggtc
    16021 gaacagcagc gtttcgctgg acatgccgtt gctttgcggc gccgacagct cgccgaggcg
    16081 ggcgtcggca ccgagtttct gcttcagcca ggcttgcagg tcgcgctgca gatgcgcgcg
    16141 gtcgcgcgtg gaggtgatgg ggcgcgccga gtcggtcttg atctccacga agcggatctc
    16201 ctgagggtat gcgtgctggg acgcgccagc atagcgccga agccgcgcac cggcgcgatc
    16261 ctgagaaaac cgtaatgcga cagctgtccc gccatggtcg cgcctggtgc tcaagccggc
    16321 gcaccggcac ggaacaggcg tgcgatgcgc gccagcagca ggcgcacgcc gcgccagagc
    16381 ttcggcagca gccaggccag cagcagcagg aacagcgcgg ccacgccgag gaacagccac
    16441 gggtgaaaca ccgcgagcca catcgcgccg atcgccagca cgtcctcgcc gagcgaggcg
    16501 ccccagttgc tgaagggttc cggcgaggtg ttgagcagca ggcgaccacc ggccttggtc
    16561 gcgtgcgtcc cggcagcaag tcccgcgccg aggatgccgg atgcgacggc gaagtccggg
    16621 ccgaccggcg ccgcggcggc cgcggcgagc agcccgccgg cgggtatgcg gatgaaggtg
    16681 tgcagcgcat cccacagcga gtcgaggccg ggaatcttgt ctgccacgaa ctctgcgagg
    16741 aacatcgcgc tggcggcgcc gagcaccagc gggtgctgca gcagttccat cccgggtggc
    16801 agctcgacgc cggcgaagcg ccccagcagg cccagcacca gcatcaccgc gtagaggttg
    16861 atgccgctcg cgagcgccgc gccggcggcg agcgcgatga gctgcaccgt atccgcgtac
    16921 tgttccatgg ggcctccgga ctgtgctgcg cccgatgcga cgggtatggg gtggatgcgg
    16981 aggctacggc gcaacccgcg cgctgcgcgg ggatttgccg gtccagcgct tgaacgcatg
    17041 gctgaagctc ggcgcgtcgg cgtagccgag gcgcagggcg atgtcctcga ccgagagttc
    17101 cgtcgcctgc aggtactcga gcgagaggtt cttgcgcacg tcctccagca gttccttgaa
    17161 gctggtgtcc tcgacggcga gcttgcgctg gatcgtgcgc ggcgtggtgc acaggcgttc
    17221 ggcgatggtc tccaggctcg cgagctggtt cggcagctgc accaggtgct ggcggatcag
    17281 ctcggcgaag ctgccgccgc gctcgagctc ggcgcagatg cgttcgcact gcgccgcgaa
    17341 aacacgtgcc gaggcggttt cgctctgcac cagcggctcg ctgaggaaac tccccgggaa
    17401 gcggaagtca ttggtcgggg cgccgaagtt gatcgggcag ccgaagaaat cggcgtaggc
    17461 ctgtgcgtag ccgggttcgg gctgcgccac gttgacctcg atcagcggca ggcgcttgcc
    17521 aagagcctcg gcggcaatgg tgtaagaggc cgcgagcccg cggtcgatca tgtactggtg
    17581 gcagccgccc aggtcgcgct gcttgccgag cgcgatgatc gccaccccgt cctcggtgcg
    17641 cagcgaccag tgcatgtagg tccaggtcat caggatgttg cgaaacagca cgccgaaggc
    17701 atcgacgagg ttcgaactcg tcatcaccgc gagccccagc atgccgtagg ccgacaggtg
    17761 gtagcgcggc cccaggcgca ggccgaggtc gggctgctcg agttgcgcga gcgcgcgctc
    17821 ggtgaagctc agttcctggg ccggactcag cttgtaatcc gggtcggcca gcgcgcccgg
    17881 cgggatgcgt gccgccgcga gcatggcctc gacgtccgcg ccgtgctggc gcagcaggct
    17941 gatcagcggg ttcagtccgt agatcaggtg ccggacattg cccggtttgg tcctggtttc
    18001 cagcagcatg ggcggcggtg cctgatggtt cgttattgca atcgcgctgt cgcaaactaa
    18061 caagttaatt gtcgtgcgta aacatctgcg cggcgccccg gctcggctat aaatggcagc
    18121 cattgcgatg gcccaagccc atcgccacga ctttgcatac cgagcaacaa cttcaacagc
    18181 cggaagcagg aggtcgccat gagcgccatc gaatggcccg aaaccccgcg gggcaagctc
    18241 acgatatcgg gggcagagcc gccgttgccg cccgcgatcg cggaattcca gaagacgctg
    18301 cgcgagttcg cggtgaacgt gatgcgcccg acgggcatcc agctcgaccg gatgagcgcc
    18361 gaggaggtca tcgcgccggg gtcgccgtac tgggacttgc gccgcaagta cctcgatctc
    18421 gggatcaaca tggagaccct cggctcgttc tcgcccgagg acttcagcgt tgccttcccg
    18481 ctgatcttcg aggagctcgg ctacggtgat gcgggcctcg ccatctcgat gggagcggcg
    18541 atcctgccga tgtatctcgc ggccaagttc ggcaaccagt tcctgctcga gcgcttcccc
    18601 gacaccctga tcggctgctg gggcatcacc gagcccgatc acggcagcga ttccctcgat
    18661 ccgaacaagc agatcctcga tgcgcgcggc cagtacggac gccccaactg cgtggcgacg
    18721 ctgcgcggcg acaaggtgat catcaacggc cagaagtcgg cctgggtgtc gaatgggccc
    18781 atcgccgagg tctgcatcct ctattgcgcc gccgataccg gcaacggccc ggacccgtac
    18841 aacggctgcg tgctcgtgct gccgatggat gcgcccggcg tgagccgcgg caagccgctg
    18901 gagaagatgg gccagcgcgc gctgccccag ggcgagatct tcttcgacaa cgtcgagctg
    18961 tcgagcgact acatcctggc tggtccggtg gcctacaaga aggccgtcta ctgcatccac
    19021 accgaagcca acgcgctgat gggcggcacc ttcaccgggg tggcacaggc ggctttcgac
    19081 ctcgcgctgg attacgcgca cgaacgcagg cagggcggcg tgccgatcat ccgccaccag
    19141 tcggtgcagc agcgcctgtt ccacatgtat cgcaaggtcg agatctcgcg cgcgctgacg
    19201 cgccgcgtgg tgctgttcaa caacaccgcg ccggttccgg cgctgcaggc cgcgatgacc
    19261 gccaaggtca ccggcaccca gaactgcttc gatgtctgca gcgacgcgct gcagctcttc
    19321 ggtggcaacg ggctgacgcg cgaatacccg atggagaagc tgttgcgcga cgcgcgcgcc
    19381 tcgatgatcg aggacggctg caacgaaatc ctgtcgatca agggcggctg ctacctgatc
    19441 gacgaagagc gcctgcgcgg atgaaacaga acgcctggat cgccggcgtc ggcatgacgc
    19501 acttcggcaa gcacatggac cgcgggctga agtcgctcgc gggcgaggcc atcaccgccg
    19561 cagtcgcgga tgcggggatc gggctgaggg acgtgcaggc cgcgtggatg ggcaatgccg
    19621 ccgcggcggt gatcaccggt caggtgtgcg tgcccgggca gatggcgctg cgcgaactcg
    19681 gcgtggggcg catcccggtg gtgaacgtcg agaatgcctg cgccacggcg gcgacggcgt
    19741 tccagcaggc ctgcacgatg gtgagcgcgg gactgtacga cgtggtgctc gcctgcggcg
    19801 ctgaaaagct ctaccacccc gacaagctga agaccttctc ggtgttcagc ggcgcggtcg
    19861 acgtcgaagc catggaccgg gtcgtcagcg gcatcgcgcg gcgcatcgag gcgctgggcg
    19921 cgccgatcga caccgcgggc gccggcacca cgcgctcgct gttcatggac atctacgcga
    19981 tctgggcgct ctcgcacatg cagcgccacg gcaccacgcg cgagcagttc gcgatggtgt
    20041 cggccaagaa ctccgtgcac ggcagcctga atccgaaggc gcagtaccgc gacgtgatca
    20101 cggtgcagga tgttctggcc gcgcgcgagg tctcgtggcc gctgacgctg ccgatgtgct
    20161 cgccgatcgg cgacggcgcg gccgcggcaa tcatcgtgag cgaacgcaag gcacgcgaac
    20221 tgggcatgcg gcgcgccatc agggtggagg cctcgcagct gagcagcggc tgggatttcg
    20281 ccgagggcga ggaatcggtg gccgagggct cggtgcgcgc gttgtacgag gccaccggca
    20341 tcggcgcgcc cgacatcgac tgcatcgagt tgcacgacgc ctccgcgcca tccgagctga
    20401 tgtactacga gtacctcggg ctgtgtgcga agggcgaggg cgggcgtttc gtgcaggagg
    20461 gccactcgcg cctcggcggt tcgaagcccg tgaacacctc gggcggactg atgcgcaagg
    20521 ggcacccgat aggcgccacc gggctggcgc agatcgtgga gctcaccgag cagctgcgcg
    20581 gcgaggccgg cgcgcgccag gtcgccggag cgaaggtcgc gctggccgag aacggcggcg
    20641 gctacatcgg tacggatgcc gcggcgatgg tgctcacgct gctgtcgcgc tgagcggggc
    20701 ggcaccccgc ggggtgccgg gcggtcagcg ggccgcgcgg gaacatgaca acaacagggg
    20761 cagatcgcac acgtgagcga cgagcgcatt ctcgccaatc tgatcgcgag gcaggcggcc
    20821 gaacatccgg acctggatgt gctgaccttc gtggacgtga cggccgatgg cggattcgcc
    20881 gaggaagtgc gcagcttccg ccaattgcgc gacaacgggc agcgcgtggc gcaggcgctt
    20941 ggcgatgccg gcatgcaggc cggggatcgc ttcgcgctgc tgatgcagaa ccaccccgag
    21001 ttcgtcgacg ccatggtcgg ctcgagcatc gccgctaccg tgttcgtgcc gatagacccg
    21061 cgcacgcgcg gcgagaagct cgcgttcatg ctcggatttt ccggatgccg cggcgtgatc
    21121 tgcgccgatt atgcgctcgg ccacctgatg gccgtgatcc gcgagctggt ggagatacgc
    21181 tgggtctggg tgctgcgcac gggggccgtg cagcacctgc ctgcatcggg cgtgagcccg
    21241 cgctggctgg acgaggtgct gcctgtcgcg gtccccgcgc tgccggtggc ggtggacagc
    21301 cccgagcaga ccatgcagat gctgttcacc tccggcacca cgggcgatcc gaaggcgata
    21361 ctcgcgaact acctgcgctt ccatgcggtg ggctccattg ccccgctgat cggctggagc
    21421 gccgcggacg tcttctatac cgggctgtcg ctcacgcatg ccaacgcgca gctgatcacg
    21481 ctgggcaacg tgctgacact gggcgcgcgc ggcgtgatca gccgccgctt caccaagtcg
    21541 cgcctgtggg acatctgccg ccgctacgga tgcaccacct tcaacctgct cggcggcatg
    21601 accaccgcgg tctacagcga gccgccgcgc gccgacgacg ccgacaaccc cgtgcgcctg
    21661 gtgctgagcg ccggcatgcc cgccgcgatc tggcagaaat tcgcgcagcg cttcggcgtg
    21721 cagatcttcg agttctacgg cgcggccgag ggcggcatga gcttcaaccc gcccggcgtc
    21781 ggtccggtcg gcagcatcgg caagccgccg cccacgctgg aggcgaggat cgtcgacgaa
    21841 gacgaccgcg agtgcgcccc gttcgagcag ggcgagatcg tgttccgcaa cgccgatggc
    21901 agctgcccgc ccgtcgagta cttccagaat cccgaggctt ccgcgaagaa gaccgccggc
    21961 ggctggctgc gcatgggcga cgtgggccat cgcgatgccg ggggctggct ctacttcgac
    22021 taccgcaagg gcggcggcat ccgccacaac ggtgatttcg tcaacgtcgc cttcgtcgag
    22081 aaggccatcg cggagcaccc cggcgtcgat gacgtcttcg tctacgggat cgaggccgat
    22141 tccggcgttc ccggcgagaa ggacgtggtg gccgcggtgg ttcccgcgcc cggcgccgcg
    22201 ttcgacgtgg ccgagctcta ccgccactgc cgcgcggcac tcgaatccaa cttcgtgccg
    22261 ggctacatcc agctcatgtc cgagattccc aagacggcct cggaaaagcc gcaggagcgc
    22321 ttctgcctgg agcacttgcg cacccacccc gaagccgtgt tcaccgaaag cagcaccagc
    22381 gaacccttgc ggagaaaatc atgaacgccg aagtcgtcgt ctacggagcc agcggatata
    22441 ccggcaagct gattgcctgg catctggccg agcgcggaat tccctttatc gccgcgggcc
    22501 gcaatgcccg tcgcctggcc gagcagatgg cgaaggtgcc ggagctcgcc ggggcgcgct
    22561 acgagtgtgt cgcggtggag catgaagagg ccgcgctcgc gaagctcttc agcggcagga
    22621 aggtggtcta caacgtcgtc gggccgttca tgcagctcgg cgaccccgtg gtgaaggcct
    22681 gtcttgccgc gggctgtcat tacctcgaca ccaccggcga gcaggactgg atgctgcacc
    22741 tcaagcgcag ctacggcccg gacttcgcgc agaagggcct gctgctggtg ccggcctgct
    22801 cgtggatgtg gctggccggg cagctcgccg cggaagtggc gctggaacac ccgggcatcg
    22861 acagcctcga catctgctat ctcgccgact cgaacaccag cgtcgcctcg acgatgtcgt
    22921 tcctgcgcat gctgaccaag gaccagttct tcctcgagca caaccggatg tgcacctggc
    22981 cgcaggccac ggcttacccg gtgtcgatcc cgggcgtgca ccgcgtgctg aatgcactgc
    23041 cgtggagcgg cggcggcgag ccggtttggt acgaggacga tgcgcgcgtg cgcaactgca
    23101 gcgtgctggt ggcgtttcgc aaccaggcga tgctgcaggc catcgtcgac atcctggtgg
    23161 agttcgagca caagcacaaa cacctgccgg tcgcggagca ggagaagatc accaacgagc
    23221 tcggcaacca gctggtatcg gtggagcccg agcgcgagag ccccgccctg aaccgcagcc
    23281 tgctgtgctg ccacgggcgc ggcgacaccg aatcggtcag cgtggtgttg cgcggcaatt
    23341 cgccgtacat ccagaccgga gtattcgccg ccgaggcgtg ccgcaagatc ctgctcggca
    23401 cgcaccgcgg caagggattc atgtcgccgg ccggcgcctt cggtgcgcgc gagatgatcg
    23461 cggcgatcgc tgccagcgga cagctcgcgt gggatacgcg tacggtctga acggatcgcc
    23521 gcgatgatga agagcaatgc aggagtgtct ggcgcacggc cgccgggggg aagcgcttga
    23581 agacgatcag gcaacgtatc tcggcgttca cgatacgcca tgcgggcaag gtgctgctgc
    23641 tgctggcgct ggtcacggcg gtgtttgccg cggggctgcc gcgcgtggag ctgcgcacca
    23701 ttttctcgga tctcttcccg agcaatcatc ccttcgtgca gaccttccgc gaccacccga
    23761 acttcggcaa cccgctcacc gtcacgatca tggtcaagcg caaggacggc gacatctaca
    23821 acgccgagac gctgcagaag atctggaaca tgacgcggga catcgacctc acgcccgcgg
    23881 tcgaccatga ccaggtcctg tccatctcga cggagaaggc gcgtttccag gaagccacgc
    23941 cctttggcat cgacagccag ccgctgatgg gcgaccacgc gccggccacc gcggaggaga
    24001 tcgccgagtt tcgccgccgc gtggagaaat cgccgaattc gcggcagttc ctgatctccg
    24061 ccgacgagtc cgccacgctg atccgcgcca ccttcatcga gcgcttgctg gattacggcg
    24121 agagcttcga gtacgtgcag gcgctggtgg agcgcgagcg cgacgcgaac cacgaggtct
    24181 acgtcgccgg gcagcccgtg ctgaccggct gggtgtaccg ttacgagaag cagatgttcg
    24241 gcatcttcgc cgtgaccatc acggcactgc tgctctcgct gatcgtctac atgcgcaatg
    24301 tcgcgggcgt gatcgggccg ctggtgacca gcgccaccgc ggcgatctgg ggtttcggct
    24361 tcgtcggctg gctgggcatg ccgatcgagc cgctgatcat ggtggtgccg ctgctgctca
    24421 tcgcgcgctc gttcagtcat tgcgtgcagt tcaccgagcg cttctacgag gtctatcacc
    24481 acgtgcgcga caagcgcaag gcggccgaga tctcgatggg cgtgatgatg gcgccgggaa
    24541 cgctcggcat cgtcaccgac gcggcgggcc tgttcctgat cgcgatcgcc ccgattccgg
    24601 caatggagcg cttcgcgctg ttctgcggct tctgggcgct gatgctgatc ccgaccaacg
    24661 tcatcatgtc cgcgctgctg ctcaccttcc tgcccgagcc gcgcaacgtt gccgcgatca
    24721 tcggtgagga cgggaagcac tcgccgctgc acgccggcat caagcgcctg ctgggggtga
    24781 cgggctcgct gagccacggt cgcaaagggc gcgtgctcgc cgtggtcgtg ctggtggtga
    24841 cggtactggc ggtagtgaag ctcggggacc tgaaggtcgg caatccggtg gagggcagca
    24901 gcctgctgtt cgaggattcg gagtacaacg tcgcggtggg gaacatcaac gcgcagtttc
    24961 ccgggctgaa cacgctggag atcgtgttcg aggccaagga ggcgagcagc gtgcgccgcg
    25021 tgatgcacca ggccgacacc atcgcgacga tgaacgcgct gcagcgcatc gtcgagaacg
    25081 acgagacccc gcccatggcg accctgtcat tcgccgatta tctgcccgaa gccaaccgcc
    25141 tgttcagcgg tggcaatccg aaatgggcgc cgctcgatcc gagcgacgaa tcggtcgccg
    25201 cggccgcggg cgcggtgctg ttcggcacca gcccgaaggc cttcctgcac gtcaccgatt
    25261 ttatccagga gaacggcacg gtctcgctct ggtacaagga caacaagcag gagaccgtcg
    25321 atcgcgcact ggcgcaggcg cgcgccgcgg tcgagaaggt cggcgcggag cacgagaact
    25381 tccgcatccg cctcgccacg ggcaccatcg cgctgcagca gtcgatcaac gacacggtgg
    25441 accggtacca gtacatcatc atggcgctgc tgaacgtcgt gatgctgttc agctgcgcct
    25501 atgcctaccg gtccttcgtc gcgggcatcc tgctgctgat tccggtcaat ctatcgaacg
    25561 tgctgcttgg cgcggtgatg gtctcgatgg gcatcggact ggacgtgaac accttgccga
    25621 tcaccgcgat cggcatcggc gtgggcatcg attacggcat ctacctgctc agccgcatct
    25681 gcgaggaata ccagctgcac gagagctacg agaaagcgat cgcggaggcg gtcggcacca
    25741 ccggaaaggc gatcttcttc accgcgacga tcgtgctgat cgcgatcctg ccgtggtatt
    25801 tcctctcgga gctgaaattc ctcgccgaca tgggccttct gctggtcatg gtgatgctga
    25861 tcaacatggt gatcgcgctg ctggtgctgc cgctgctggt gtggctgttc aagccgaagt
    25921 tcgtcggttc gaaggacctg ctggtcggcg agggcgtgga cctgtcggat tacgcgacgt
    25981 gagaatggtt tcgataacga gtatttccac tggaggactg acccatgcgc atcagaaaat
    26041 atgacttcaa ctacagtcgc cgtgccttcc tggacaagat ggcgaccggc gcgatggccg
    26101 ccggcgtgct gagcccgctg tggccgctga tcgcgcgcgc cggcgacatc accaaggcct
    26161 accccgagga actgctgtcg atcgaggcgt acaccaaggg caagatcaag accggagacc
    26221 tgatcaccgc cgacaacgtc gagcacgtga aggacctgct cgacccggtg gcctatgtgc
    26281 acgtgagcca gatgggacgc cagatccgca tcgtgaagac caccaccgac gccacgcggc
    26341 tgttcccgag gaagtatctc gaggcgacgc tgaggaacca gggcaaggcg cggctagatg
    26401 ccgacggcaa tgtagtcacc gccgagggca agccgtggat cggcggcaat ccgttcccgg
    26461 atcccaagga cgggctggag gccttctcga acctgaccct gtcctggggc cgccacgaca
    26521 actccttcta cgcggtgcgc gactgggaca tcgggcccga cggggacctg cagtacgaat
    26581 acgacttctg ctgggccgag cagaacaccg tggcgctggt gggggagaac ggtccctaca
    26641 tgccgggcca cgaggacaag ctgcgcttcc agtcggtgtg gttcacgtat cccaacgact
    26701 ccaagggcac ctccttcctg aatacctggt actacgacca gcgcaagttc cccgacctgc
    26761 agggttacct gccggcgttc aagcgcgtgc gccgattccc caccaaccag cgcttcgaac
    26821 cgctggtgcc cggaatcacg ctgttcctgt ccgatgcctg ggcagccggt gacccgatgc
    26881 tgacctgggg caactacaag gtgatcggtc gccagccgat gctcggcgcg gtatccgaga
    26941 actggatggg acccgacacc aactacgagc gcccggttca cggcggtgcc aagggcaaga
    27001 ccttcatgga gaccgcgatg gaactcgtac cagagaccat cgtgatcgag gccgagccca
    27061 ccggctaccc gcgcgcgccg gtcggcaaga agcgcgtgtg gatagacacg cgcaacatga
    27121 tgtacgtggc ctacgtgacc tacgaccgcc gcggcgaact ctggaagagc ttcgagccgg
    27181 cttactcggt ctacgaaaag ggcgaccaga ggctgatgga tggcgccgag ccggcgtggt
    27241 cgtggacgca cgtgcactgc cacgacgtgc agaccaaccg catgagccgc ttcgtgcagg
    27301 ccgaggaagt gcgcggcggt ttccgctccg gcttcaacat cgagggcatg tacgacaagt
    27361 acctcacgat gcaggcgctc ctgaggatgg gtacctgatg cgccggcgac gggagccggc
    27421 ccgcagctgc gcgacgctgc tgctggcggc gctgtgctgc gccgcggccc gcgcagcgga
    27481 gcccgtcgtg ctgcaccagg gcatcgtgca cgatgcgctc tacgacgtgt gcttcgatgc
    27541 gcgcggcgcg gtggcggtgg gtgcggcggg cgtggtgctg aagagcagcg acgcgggcgc
    27601 gagctggcag ccccagccgc aggaggccac ggcactcgcg ctgctcgggg ttgcctgcac
    27661 cgacgagagg cggctcgcgg tcgggcaggc cgggaccgtg ctggtcgatg acggcaacgg
    27721 ctggcgcaag gtcgaaaccg gcaccgagca gcgcctgctg gcggttgcca tgaacgactc
    27781 ggggctggcg gtcgcggtgg gcggatttgg cgccgtgatg ctttcccgtg accacggcga
    27841 aagctgggaa ccggtcgtct tcgactggcc ggcgctggtg gaggatttcg ccgagccgca
    27901 tctctacgac gtcgcggtcg ccggggacgg cgcgatcacg ctggtcggcg agttcgagat
    27961 cgtgatgcgc tcgcccgacg gcggcgccac ctgggcccgc gtgcacaagg gcgatgcctc
    28021 gctgttcggg ctgcacatcg ggccctcggg agtgggttac gcggtcgggc aggaaggacg
    28081 catcctgcgc agcgccgacg gcggcgcgag ctgggaggcg ctggcagcgc cggtcgacgc
    28141 accgctgctg ggggtttggg cctccgccga cgatcgcgtg ctcgtgagcg gcatgcgcac
    28201 catgctcgcg ggcagtgcgg gcggcgcaag ctggcgcagg ctcgaaggtg ccgacatcgc
    28261 gaccgggtgg tatgccgccc tcgcgccggc aacggcggat gcgcagcgca tcgtcagcgt
    28321 cggcaacgct ggccgcgtgc tgggtttcgc gccgacggtc acggcagaag aagacgataa
    28381 acaccaacca tgatggaggc ttccatgagc aaaccacgca gactcgctgc agcgatacgc
    28441 ggtgtcacgc tggtctcggc ggcgctcgcc gcgcccgcgg cgctcgcgct ggacctgaac
    28501 gtctcgggct tcatccgcca ggagatggcc tacaagatca ccgacgacga gaacccgttc
    28561 aaccagcagg gcaacctgta caacggcaag ccggtggaca acttcgtgca cggcctctat
    28621 ggcctgccac cggagatcac gcgccccgat tactccgagt ccaacgactg gaacctgcag
    28681 gtgacgcgcg gcgaactgga tgtcgcggca accttcagtg aacacctgac cggtgccatg
    28741 aaggtgcgcg cctactacga ccacggtgtc tacgacgatt tcggcgacgt gaatttcttc
    28801 gaggtaccgt accgcgggga ttgcgcgacc cgcctcgagg tctgcggcga cgactacatg
    28861 atcgacctgc cgtcgctgta cctcgattac accaacggcg gcttctggac gcgcatcggc
    28921 aaccagcaga tcgcctgggg cgagtcgatc tttttccgcg tgctggacgt tccgaacggc
    28981 ctcgacctgc gccgacacag ctttctcgac tgggcctcgg aggaatattc cgacgagcga
    29041 gtgccggggc ttggcattcg ctcgagctac cgcttccaga acaactggga gatcgagggc
    29101 ttcgcgcagg aattcaaccc cacgatctac gccaacgaga acacgcccta caacgtgatc
    29161 gcctcgcagt tcgtggtgca gcaggaagag accttcgacg aggtcgacga tgaatggaac
    29221 ttcggcgcgc ggctgcgtgg ccagatcggc gagctggggc tgcagttcat cgcggtcagc
    29281 cggcgcaatc cggatggcgc gttccgctgg accacgtcca aggtgaaccc cttcgtcgcg
    29341 gccggtatcg gcgcggccga ccccgatctg ggcggcgtga cgcttgggca gttgctggcg
    29401 cagacgccgt tccagccctt caccggccag ggcgtgttca ccgcgcgcga gtggttcgac
    29461 tattcgggca tggcgcggct caacggcgcc aacctgcaac agttgcttga cgacttcccc
    29521 gcggccaagg ccctgacggg tgccaacggt ctgccgacca ccggggtcga caatctcgcg
    29581 acctccacgc tggtgctcga tgccttcttc tcgcccatcc tcggcctcgg cgacctgcgc
    29641 ggccacatcg agcgcaagtt ccatcgcgag gagatcttcg gcataggcat gaactacatg
    29701 ttctatgccg agccggattc cctcctcgac cagctgatcg tgcgcttcga ggcgactgtc
    29761 acgccggaca agatgttcac cgatccttcg ctcgggcagg aattcctcga ggaaaccgag
    29821 tgggcgacct cgctggtgtt cgagaagtac cagcgcttct ccgagacctt cccggcgacg
    29881 tacatggtgc tgcagtggtt gcacaagtcc gagagcgacc tgttcgggcg tcatctcgat
    29941 ggcttcgacg gggatgcgag tcgctcgccg cccgccggcc gtgaccagtt cgacgcgata
    30001 gccttcgcga tgcagcagcc ctcgccgacg ctgatgtggc gcttcgacct tgcggtgctc
    30061 tacgacgtgg agggcggcat cctggtgcag cccggcgtgc gttggaagcc ctcgaagtcc
    30121 tacacggtcg agctctacgc gaacctgctg gaaggggata acaacgactc catgggcacg
    30181 ttccagtggt ccgatgaggt cggcttgcgc ctgggctatc agttctgatg gacgtgctcg
    30241 agcagggcat gtcgctggcg cgcctcggcg gaccggtggt cctggtgcta ttggcgatgt
    30301 cggtggttgc cctggcggtg tccgctttca agatgctgca catcggcacg ctcacgcgcg
    30361 gctcgggcgt ggcggagcgg gcgctcgatc actgggtgcg tgacgatacc gatgcggcgc
    30421 tcgcgctgct gggacgcacg cgtggcgagg cgccggcgct gctgcgtgac ggcatgcaat
    30481 ggctcggcgc cggcaagccc gcgccgagcg tgcgcgagga gctggtgcgc cgcgccacgc
    30541 gcctgctcgc ggatgcgggc aacctgctcg gcgtgatcga acaggtggcg tacctcgcgc
    30601 cactgctcgg gctgctcggc accgtgctcg ggatcatcga cgtgttccag ggtctcgcgg
    30661 cgcacgcgga tgcgagcgcg ggaacgctcg cgggcggcat ctgggaagca ctgctcacca
    30721 ccgcggtggg gctgtgcgtc gcggtgccgt tcgcgatcgt gcacgcggcg ctcgagaacc
    30781 gcatcgagcg cttccgtgcg cgcatggagg acgcgctgac gcggctgttc accgtcacct
    30841 tgtaccgggc tggttgagac gcgggaggca cgccgatgct gcagtccgtc cggcatgcac
    30901 actccgccaa gcgcgttgcg atgacgccgc tgatcgatat cgtgttcatc ctgctgctgt
    30961 tcttcatatt gcagagcaat ttcacgcagt tccgcgagct gctgctgccc gtgcccgcga
    31021 cggcggaggg cgcgcgggag ggcccgcgcc agctgcgaat cgaggtgggc gcagacggtc
    31081 gcctctggat cgacggcgtg acgctcgccg ccggcgggct cggggagttc ctggatggcc
    31141 gggacctgcc tggcgcaacg cccgtggtgc tggtcgcggc acccgcggtg ccggtgcagc
    31201 tgctggtcga tctctgcgac acgctgcgct cgcgcaagct ccaggggatc gatatccgcg
    31261 cgctggaggc gccctagcgc catgatccgc ctgggacggc cggcgcggcg cgccaaggac
    31321 cacgacgaca agctcgtgcc gatgatcaac gtgatcttcc tgctgctgat gttcttcctg
    31381 atcgcgggca acctgaaacc gctgttcagc gaggagcagc tcataccgct ttcgcgcagc
    31441 gaggcgctgc gggcgcacgc gcggatcgaa ctttccctgg cgcgcgacgg cgtgctgacg
    31501 tggggcgacg aggcgatggg cgcggcgcag ctgggcgcgc gcctgcgcgc gctgcccgat
    31561 ggcgtgcccg ccgcgctcag cctgcgtgcc gacgcgcgcg cgcaggcctc ggcgctgctg
    31621 cccgtgctcg atgcaatgcg tggcgccggc gtgcagcgta ttgccatcgt cacgctgcgc
    31681 cgctgaggcc gatgcgcccg cgccgcctgc tgctctggat cgcgctgctc gccgcttcgc
    31741 tcgtgctgca tttcgcgttg tcgtggcagg ccgggcgcga gctcgggctg cccggcgccg
    31801 gggagcccga gcaggcgagc gtcgccggga tacggctggc gccgcaaccg cccgagccgg
    31861 agcgcgagga gctggtgttc gaggcgcctc cctccatggt ccagcccgcg gcgctgctcg
    31921 cctcgaccgc tgccgtgacg ccgccgccga acgtcaacct cgcgctgcgc gccgcggccg
    31981 gagcgggcag cgagatgccg atgccggcgc cggtgcaggg ctttggactg cagcaggcgc
    32041 gcggcatggg cgctgcgggc ttcggcacgg gtatcggcaa cgggctgagc aattcgagca
    32101 accgcttcgc ggcctacgtg cagggcctgc gcgacaccgg cctcgacgtg gtgttcgtga
    32161 tcgatgccac cggctccatg gactgggtgc tggtggaagt caggaagcgc gtcgctgaca
    32221 tcgccgatgc cacgcgctcg ctggtgccgc tcacgcgctt cggcgtggtg gcctaccgcg
    32281 accgcgacga ccccgggttc gtggtgcgcg agcaacccct gaccttcagc ctcgcgaagc
    32341 tcgcgcgctt cgtcgacgcc ctgcgcgccg agggcggcgg ctcgtggcag gaggcggtgg
    32401 atgccgggct cgaggccgcg gtgaaccgcg ccggctggcg tcccggcgcc aagcgcgtgg
    32461 tgttgctcat cggggacgca ccgccgcacg agaaggacat ggagcgcacg ctggcactgg
    32521 cgcgcgaact cgcgcgcgcc ggcggcgaga tctcggtgct cgatgtcagc aacgacgcca
    32581 atcccgcgct gatcgaggcg agcctggggc gcaaggtcaa tcgcgcgctc taccgcgagc
    32641 gcccgatgct gcagtacgag agcatcgcgg aggccggtgg cggcacggcg gcgaccatgg
    32701 atggcgacat ccgcatcacg cgccagctgg tctcgctgat catgggcggg cagttctccg
    32761 ccgagatgag cctgctgctg gaggggctgt gatgcgaggg gcgcactcca ccgccgccgc
    32821 gctgctggcg ctgctgatcg cttgcgtggc gctgctggcg cgtggcgagg acgacgccgt
    32881 ccacgccttc gccgcgcagt tgcccgaggc cgcgcaatgg gacgccgccc gcttcagtga
    32941 gcgtgcggat gccttcggca agctggtgag cgcggcgcgc gacgccatgg ctggtgcacc
    33001 ggcgcagcag cgcctgcgcg cactcgggga cgaactgcac gctgcctgcc gcgccaggat
    33061 ccgtgcgctg gaggccgcga ccggcgagga cgaggcgaaa ctcgaggatc tctaccgctc
    33121 ggagcagtgg tacgcgatca actacgcgct cgccgccgta cgctactggc aggcgtggat
    33181 cgatctcgcg cttgccggga ccgccggcga cgacgcagcg cgcctgccgg cgttgtcgcg
    33241 agccgaacgc ggtttccagt ccaccgcggt gcgcatcctg tacccggggc tggtctacgg
    33301 cagctggctc ggcatggcct atgtcgagcg cttgcgcggc gatgatgcgg cggcgcagag
    33361 ccgcctggag ctgctcgccg gcgcgctcgc cgccgatccc ggcaacccgc tcgcgaaact
    33421 ggtcgccgac gagctcgcgt tgctcgccgt gcggcgcggc gaggccgtga gcgcgttgcc
    33481 ggcgggcaag ggcaggctgg aaccggctct cgcgcgccgc ctgcaggagg aagccttcgc
    33541 cctgctggaa aagcgccgca gcgacggcat cgcggcactg gaggccgcgg agcgcctgcg
    33601 ccgcctggtc gatgcgggat acctcgacga tgccttgctc gcgcggatgc tcgcctggcg
    33661 caacgagctg gtcgggcgcg atgtgggcac catcggcctg ctgctggacg ccgagtacgc
    33721 cgccgcgcac gagcagtacg aaaccgccgt gttgaagtac cgcgagttcg cggccaaagg
    33781 cggtttcgaa ctgcccatcg acagcgcgcc gttgcgctac aactacgccg tcgcgctgca
    33841 tcgcctgggc tacgaacgcg aggccctcga ggtggcgatg caactgcgcg aggaccgcgc
    33901 cgttggtccg gcggcgcgca agctcgtctt cctggccgcg gagcgcatca accgcgatca
    33961 acccggcgcc gcgaaccgcg ccgccctgag tcgtgccgcg cgcgatttcc ttgcggcctc
    34021 gcccgatgac aaggacgccg ccttcgcgca cctggcgctc gccggcggcg gcgacgccgc
    34081 ggcgcggact cacctcgccg cggcgcgccg cgacccgcgc acgcgcgccg cggtggaggg
    34141 cgtgcgcctc gatgccgcgg ttgtcgcgtt caccgctgcg gcggcgcgca atgacctggc
    34201 cgcgcgcgcc agcgccgcgc gtgaggcgct ggcggccatc gatgccctgc cgcgcgccga
    34261 gcggcacgcg ctgccgttgc gcgtgctcgc gttgcagatg cgcgtcgcca gcggcgatac
    34321 cggcgagcgc accttgcgcg agctgcagga gctgcaactc gccgcaggca ccgatgccgc
    34381 gctgctgcgc gtgctggcgt ggagcaggct gcgcgcgctg tccggcgatg cgctgtcggg
    34441 gtacatcggc gcgctggggc gcccgccgtc caccgccgat gcgaccgaac tggtggcgtt
    34501 cctgcaggag cgcgagcagg cacgcgattt cgcgacactg caggagctgg cggagcgcgc
    34561 gctgccggcc ttcgcatcgc aacccgacgt ggcgcgccag ctggtgctga tgcgtcatcg
    34621 cgcgctcacc gcgctcgagc gggagcagga agcgttcgtc gttgcgcagg agatggtcca
    34681 ggcctacccg gattcgggcg atgcgtggct cgcctacgcc gaaagcgccg ggcgcagcgg
    34741 cgacaccttc gccgccgatc gcgcctgggc ccggatcgcc accgccacac ccgagggctc
    34801 cgaccgctgg ctcgatgcca cgttgcgccg cgtggccttg ctgtcgcgta ccgcgggcgc
    34861 gcaagctgcg cactgcgatc tgctggcccg cgcgggcctg taccgggagc gcatgaatgc
    34921 cgcgctcggc aagcaactcg ccgaactgga gcgggagaat cgctgcgatg gctagctgtg
    34981 gtggtgcgcg ggttcgagca tgcaggagcc tcgccgcggc gccgggcgat ggtctctcgt
    35041 atatggcgca caaaccagcg caggagggag cagcgccatg gtaaggatcc gcaagtacca
    35101 cgccgactac tcgcgccggc tgttcctgca gcggctgggg cagggaatgc tggccacggg
    35161 cgtgctgatg ccgctgtggc aggcgattgc gcaggacggg gacagcgcgc gcgcgtatcc
    35221 ggacgaagcg ctgtcgctcg aggcttacac gaagggcaag gtgaaggccg gcgacgtgat
    35281 cagcgccggc aacgtcgagt atgtgaggga cctgctcgac ccgatccagt acctccaggt
    35341 cgcgcagatg ggccgccggc tcgacgtgct ggacaccacc acggacatca tgcagctgag
    35401 cccgtgggag tatgtcgaag cgacgctgcg caacaagggg cgcgcacgct tcgccccgga
    35461 tggcaacgtg gtcaccgacg agggcaggcc ttggatcggt ggcaacccgt ttccccagcc
    35521 cggcagcgcg ctcgaggtat tcgcggggat cacgctgagt tggggccggc acgacgtgtc
    35581 gctgtacgcg acaaaggaag aggacctcga cgacgacggc gacgtggcgt accgctacga
    35641 atcggtgtgg accgagttgt cgccggtggc gcgcgtttcg ctggatccaa agccctactg
    35701 gcccggacac gaggacaagc tgcgctacca gtcgatcctg ttcctgtcgc cgaacgatgt
    35761 gaagggcacc tccttcctga acatctggca ctacgaccag aaccgctatc ccgacctgtt
    35821 cggctacctg cccgccttca agcgtgtcag gcgctacccc accaaccagc gcttcgaacc
    35881 gttgattccc ggctccacgc tgtatctctc ggatgcctgg gccgctggcg accccttcct
    35941 cacctggggc aactaccgca tcgtgcacca ggggccggca ctgggcgccg tctcgcgcag
    36001 ctggaacgcg acgcacccga actgggagca caccacgcat ggcggtgcga acgggctgtc
    36061 gttctgggat accgcggtcg aattcatccc cgaggccatc gtggtcgagg ccgagccggt
    36121 gaaatacccg cgcgcgccgg tcggcaggaa acgggtgtgg ttcgacgcgc gcacgctgct
    36181 gccgctcagc atggtgagct tcgaccgcca ggggaagatg ttccgctcct tcgatggcgc
    36241 gttctcgctg tacagcgacg gcagcaacgc ggtgatggat ggcgcgcatc cgtactggtc
    36301 ctggacgcgc gtgcacgcgc acgacgtgca gacgaaccgc atcacgcgcc tgcagcaggt
    36361 gcgcgaactg cccggcggct accgcaccac cgtgaacgac cagggcgtct acgagaaata
    36421 cctgacccag tccgcgttgc agcgcctggg cagctgagca gaaattttgc aggggcgcgc
    36481 catgcgcgcg gccatcgggt ttcacaagag aggagatctc cgcatgacac gcaagcacaa
    36541 gctctgggcc ctgatgcccg cactgccaaa ggacctgctg gtggccagcg tgcgccagta
    36601 cgaggacgcc ggtctcgaag gcgtgtggtc gccgcagctc tacggcgcgc cattcgtgcc
    36661 gctggcggcc gcggccgcgg tgagcgatcg tctgaagctc ggcaccggcg tggcgctcgc
    36721 cttcgtgcgc agcccgctgg agaccgcgag ttgtgcgctg gacctggaca tgatctcagg
    36781 cgggcgcgcg gtgctcggca tcgggccgag catcaagtgg tggaacgagg actggtacgg
    36841 cgtgcgctac ggcaagccgc tcgcgcacct gcgcgaggtg atcggcgccg tgcgccagat
    36901 catcgccaag gggcacaccg gggagctcgg gcgcatcgac ggcgagtacg tgaagctcga
    36961 cctcgagcgc ttcaagctgc tcgcgccgcc gctgcgcacc gagatcccga tctacatccc
    37021 ggccgtgttc gagaagacct gcgagatggc cggcgagatc gccgatggcc tgccggggca
    37081 cccgatctgg aacgcgaaat gggtgcgcga caaggtcgcg ccgcacctgg ccaacgggct
    37141 cgcacgctcg ggtcgagcgc gcagggactt cgacctcaac ctgttcctgt tcgtcgcggt
    37201 gaacgaggac aagcgccagg ccatcgagga tgcgcgcgag acgatcgcct tctacggcca
    37261 gatggcccaa tacgaggcct attacgagta catcggcttc ggcagcgagg cgcgcgcgct
    37321 gcaggctgcc ggtgcgcggc aggaccgcgc cgcgctgatg gccgcctgca gtgacgagat
    37381 ggtcgaatcc atcgtgctgg cgggcacgcc ggaggaggtg cgccggcgcc tcgacgcgat
    37441 gagcgacatc gcggattcct tcacgctgtc gattccgttc tacgggctcg cacccgacaa
    37501 ggcggcgtac tacacacagc ggattgccga ggtcttctac ggctgagccg atgtgggtcg
    37561 ccattcatgg cgacagcagc ttcgcgaaag gcacatgtcg gcatgaaggc cgacccacag
    37621 gaatgccgac ccacatcgga ggaggaaaga tggaaccact gcaggctttt tcgctcgccg
    37681 gcaaggtcgc gctggtcacc ggcgcggcca gcggtatcgg gctcgagtgc gcgcgcgtgc
    37741 tcggcatggc cggcgcgcgg gtgatgatgg gtgacatcga ccattcgggc tgcgccgcgg
    37801 cggccgcggc cctgcgcgcc gcaggcctcg aggtgcaggc gcgcgcgcag gacgtgaccg
    37861 ccgaaggcga ctggcaggcc agcatcgagg ctacccgggc agccttcggc ggattcgacg
    37921 tgctggtgaa caacgccggc atctaccagg gcggcacgct ggaatcgaat acgctggatg
    37981 aggtgcggcg cgtgcaccgc gtgaacgtcg attcggtctt cctcggcatg aaattcgccg
    38041 cacaggcgat gaagccgggt ggcgcggcag ggcgcggcgg gtccatcgtg aacctgtcct
    38101 cggtggcggg gctgatcggc gtgccggggc acacggccta tggctccacc aagggcgcgg
    38161 tgcggctcta caccaagcac gcggcggtgg aattcggcgc actgggttac ggcatccgcg
    38221 tgaactcggt gcacccgggc ttgatccaga ccgcgatggg cgagaaggtg ttcgaggatt
    38281 tcatggccct cggcctcgcg cccacgctgg atgaggcgaa ggccgtggtg ctcggcatga
    38341 ccgcggtggg gcgcctgggg acgtcgcgcg atgtcgccaa catggtgctg ttccttgcca
    38401 gcgacgcgtc gagctacgtg acgggagcgg aattcgtggt cgacggcggc atgtccgccc
    38461 gctgaaggag cacaagcgat gagaatggcg aacaaggttg cattgatcac gggcgcgggc
    38521 tcgggtctcg gcgaagcgat ggcgcggcgc ctggcgcagg aaggcgcgct ggtggtggcc
    38581 tcggatatcg atttcaccgc cgtgcagggc gtggtggcct cgatcgtggc cgccggcgct
    38641 cgggccgagg cggagcatca ggacgtcacc gacgaggcgc gctgggccgc gctcgtggac
    38701 gccatcctcg cgcgacacgg gcggctcgac gtgctggtga acaacgcggg catagcgctc
    38761 accggttcgg cggaagacac cacgctcgcg gactggcagc gcacgcagcg cgtgaacctc
    38821 gacgcggtgt tcctgggcac gcgcgaggcg atccgcgcca tgaagacgcg cggcggctcc
    38881 atcgtcaaca tctcctcgat cgagggcatc gtcggcgagc cgctgactgc ggcctacaat
    38941 gccagcaagg ggggcgtgcg catcttcacg aaatcggcgg cgctgcactg cgcggccgag
    39001 ggctacggca ttcgcgtgaa ctcggtgcat cccggcttca tcctgacgcc gatggtcgag
    39061 aacgcgctgg ccggcatgcc ggcggagcag gccgcggcat tccatgcccg gatcatcgac
    39121 agcatcccgg tgcgcgcgat gggcgaagcg ctcgatatcg ccaacggcgt gctgttcctt
    39181 gccagcgacg agtcgcgcta catgacggga tcggaactcg tgatcgacgg cggttacaca
    39241 gcgaggtgat gaagatgttc gcgtatacga tggagcacat ctgcagctac ggtgtgacgc
    39301 tgcagtcgcc gttcgaggtg atcggcgaaa caccgctcgg cttgcgcatc aacgcctacg
    39361 tgaccggtgg caccgtcgcg ggaccacgca tccgcgggga gttcctgccg gtgggcggcg
    39421 actggctgac ggtgcgcagc gacggcgtcg gcgtgctgga cgtgcgcgcc acgatccgca
    39481 cgcatgacga ggcgctgatc tacgtgcagt accagggcgt gctggaactc ggcaacgacg
    39541 gctacgcgcg catgctggcg ggcaatccgc cgccgtgcgc gcagatccgc tcggcgccgc
    39601 ggttcctcag cgcgcacccg gggtacctct gggtgaaccg gctgcagtgc gtcaacatcg
    39661 gcgaggtcga ttttgcctcg gccagtgtga gctacgacgt ctacgccctg ggctgaagcc
    39721 gggcgccggc gcgcggcgcg aaatgcgctg ggcggctacg agcttcgatc agatcaagac
    39781 gtggagccgt ggattacggc agaattcgcc gcgttgcgac aatgatccga ggagcacacg
    39841 catgccgcat cccctggccg gtcagccggc gccgaaggag tacctgaccg acatccccgc
    39901 cttgctggcg gcctatgccg ggcgccagcc cgataccggt gatgcggcgc agcgcgtcgc
    39961 cttcggcacc agcggccatc gcggcagcgc gctggcgggc agtttcaacg aggcccacgt
    40021 gctcgccatc gtgcaggcgg tgtgcgatta ccgcgcgcag gcaggcatcg ctggcccgct
    40081 ctacctcggc aaggacacgc acgcgctgtc ggcaccggcc gagcgcaatg cgctcgaggt
    40141 gctggcggcc aacggcgtcg atacgcgcat acaggcgggc gacggcttca cgccgacgcc
    40201 ggtgatctcg cgcgcgatcc tggcgtggaa ccgcgcggcg tccgcgccgc gcgccgacgg
    40261 catcgtgatc accccctcgc acaacccgcc gcccgacggc ggtatcaagt acaacccgcc
    40321 gcacggggga ccggccgaca ccgacatcac cggctggatc gaagcgcgcg cgaatgcgct
    40381 gatggctgcg ggcaaccgcg acgtgaagcg cgtgccttgc gcgcaggcca tcgccgcggc
    40441 cagcacgacg caggaagact tcgtcgggcc ctacgtcgag gacctggaaa acgtgatcga
    40501 catggcggcg atacgcgcgg cgggcgtgcg catcggcgtg catccgctgg gcggcgccgc
    40561 ggtggcctac tggcagccga tctcgggcag gtacggcctg gacatcgagg tggtcgaccc
    40621 gacggtggat cctgcgttcg cgttcatgac gctcgacaac gacggcagga tccgcatgga
    40681 ttgctccagt ccgcacgcga tggcgggcct cgtcgcgttg aaggatcgtt tcgatatcgc
    40741 ctggggcaac gacgccgacg tggaccgcca cggcatcgtg acgccctcgg cagggctgat
    40801 gaacccgaac cactatctcg cggtggccat cgattacctg ctgacgcacc ggccgcagtg
    40861 gtccgcgcgc gcggcggtgg gcaagacgct ggtgtcctcg gcgctgatcg atcgcgtggt
    40921 cgccgggctg gggcgcacca tgctcgaggt gccggtgggc ttcaagtggt tctcgggcgg
    40981 tctgctcgag ggcagcttct gcttcggtgg cgaggagagc gcgggcgcct ccttcctgcg
    41041 ccgtgacggc agcgtgtgga ccaccgacaa ggacggcatc atcctcggcc tgctggcggc
    41101 cgagatcacg gcggtgaccg gcagggatcc cggctggcac taccggcggc tcgtcgcgaa
    41161 atacggcgcg ccgtgttacg cgcgcatcga cgccgcggca acgccgggcg agaaagccgc
    41221 cttcaagcgg ctcacggcgg agcgcgtcgc ggccaccacg ctcgcgggtg acccgatcac
    41281 ggcacggctg acccacgcac ccggcaacgg tgccgcgatc ggcgggctca aggtggtcac
    41341 ggccaacggc tggttcgccg cgcgcccctc gggcacggag gacgtctaca agatctacgc
    41401 cgagagcttc gtcgacccgg ctcacctgca gctgatcctg caggaagcgc ggcggatcgt
    41461 gggcgaggcg ctacgcggct gaagcggata tcgcgcgcag ggcgcgctcc cgtttttcag
    41521 aagattgcgc caatgatccg gtcgagcagc tgctccagcg ggatcatcgt gaacaccagc
    41581 ggcacgaagg gtatcagcat cgctgcggtg agctggacca gcatggtgcg cgagaagatg
    41641 aggggcttca tctcgcggat cacggtgaac gcattgccga tgtccgctag cgactggatg
    41701 tcgccggttc cgagcagcgc ctcccggtcg tggtcccccg agcgcagcca gcgccggtcg
    41761 aactcgcgca tgtactcggc ggtgaaagcg ccgtactccc tgagcccctg gcgctgagcg
    41821 gcgaggatct tcggcgcgaa caccagcagg ggagccatca gcagcaccac gcccatggtc
    41881 acgagcagga cgatttccag cttgaaggcc gcgagcgagg cgccctggtg gaagatgcgg
    41941 ttcgcgatca ttccggccac cccggcaccg agcgcgctca gcaacggcgc gaaggcgtag
    42001 gcactgccgc cgagaaagcc cagtcccccg ttgcggtcgg gatgggtcgg gatcaggtcc
    42061 agctcgatcc gcgacacctg ccacaggaag tgcgcccaga tcagcagacg gtagccccag
    42121 cgcagcagga tgaactgcag caccggattg ctcacgaagg tgaaccagag ccccgccgcg
    42181 gtcagcgtgc cggtgccggt gccggtgccg gttcctaccg tcgcgtacca gctcgaggtg
    42241 tcgagctcga tgtagtcgcg gatgtaatgg cccaccgtgt acacgagcgc gatcagcgcg
    42301 agctcgacgc gccacgaatt gcgccaggcc atggcgctgc gcaaggcctc gatgaaccgt
    42361 tccatcgatg ccgcgggaac gagcctgcgt tccacgaatt gcgccacgat gccgcgcagg
    42421 cgctggtgga gcacgagctc ggccaggatc atcagcggca cgccgaccag aaagcgcgcg
    42481 tgggtttcga tgtcggggag gaacggtatg tcgacgccgc ccagcagtgt tccctcgatg
    42541 gcgcacagca cgagcagcgg cagccagatg atcgcgctgg ccagcacgat gcggcgcgcg
    42601 accaggctgc ccgcgtcatc gtccatccgt gccttgcgca gcagctgcca cagcggtccg
    42661 ccgaggacga gcgagaagtt ctgcagttcg ggaaacgcaa cggcgacggg gtcgtcccgg
    42721 cgtgaaggga ggtctggatt catgcggcgc tttcccccgt tcaggctgtc atgtggcgga
    42781 tatcgcgttc ggcctgcccg cgccgtgtca gggcgagggc atcgcgtcac aggataattc
    42841 atccataccg gtcagcgcgc cgagttcccg cagcgtggcc tcgatcgtgg ccttccaggg
    42901 cgaatcatcg gcaagccgtc gcgcaacggg ctgcagcagc gcctcggcac ggcgcggaca
    42961 accgagcgcg ccgagcaccg acgccaggtt gttcgcgagc gcgggatcgt ccgggttgcg
    43021 cgccagtccg tcgcggtaat gcgcggcggc acgcgccggc gccttgtcgc gataggccag
    43081 attgccgagc gcgagaggtg gcagcggctg ctccggccag gcgtcggcgg cggcctgcca
    43141 tgcacgcgtg gcggcatcgc ggtccgcaac gcgctcgaaa gccgtggccg tctcaaggta
    43201 gcgccccgga tcggcgccgg cgggcagttc gcccggcttc agggcgacca tcgcccagcg
    43261 tcccgcccag tcccaggtac gcaggaattg cggcgcgtgc acgggcagct cgcgttcgct
    43321 gccgctgttg agatacaggc ggttgcgtgc ggcatcgaag ccggtgagca ccgcgtaatg
    43381 ccacaccggc acctgcggcg tgccgagatt ctgcagtacc agcaccggac ggcccgcctg
    43441 cagttgcccg agcagcgcgc gcggctcgcc cgccagcacg tagggcagcc gtccggcgcg
    43501 ccgcgtcgcc gcgacgagct cgagctgcag gctgccctgg cgcgccggca ggtatacctg
    43561 cggcgcgagg gtcgcgggat cggtcgcgac ccccgcggca ccgagcacgc cggcgagcgc
    43621 ggcagggccg cattgaaact cggtctgggc gaagaacggc acggaggaca gccgcaccgg
    43681 tgtcgcggcg ggtgcgatgg tgtcgagttc gagcggcggg ttgagcgagc agccggccag
    43741 cagcgccagc gccagcatca ggacgcgcag ccggccgggg ccggccgcgc gccaggcgcg
    43801 gcgcatcgat cagagtttct tgaagatatc gatcacgccg gtgatctcga ggatcagcag
    43861 caccaggaat accgcaccga ccaggccgag cgcgctgccg ccggcgggca gctgctgcag
    43921 ctggccttcg agccgcgcca gctccgcgtc gctcagcgag gccacgcgca gctgcgcctg
    43981 ctgtggctcc acgccgagct cgatcatcgc gctgcgcacg tcctcgcgcg ccagcgttgc
    44041 gctcaccgcg gcgatgcgtt gctcgcgcgc gtccatcgtc accgcctgtt gcgtatcgat
    44101 gaccgccgcg gcgaccccgg tgctgaatcc cgcgcagaca aggctgcccg acagggcgat
    44161 tgcgaccagt ttcttgcgca tgttccattc ctcccggacc gttgcttttc gtgcggcgcc
    44221 cgaagcgcga ggcgcgagca taagagcacg gtttggccat gtccaccgtg aatccgcgcc
    44281 gcgggccgct cgcgtggcgc aggtcacgga ttggcgtggc gcgtggtggc gcttgccgtc
    44341 gttcaggcct gtttcctgcg cgcgacggcg ggcctggcct cctgcatggc ggcgcgcacc
    44401 agcgcgcgca cggtccagct cttgagctcg cgcgccgcct gctcgtcgat ctgcaggata
    44461 tcgctgcaga cgatcatcga ctcggtgccg aagaccagcg ccagtgcgcg gcagagtcgc
    44521 cggaaggcgg ccggatcgag ttcggcgcgc gccggttcca gggcggcctc gatcagcggt
    44581 gtgcggcggt tctggcgccg gggcaccgcc tcggccggct cccgggccag cgagtgcgcc
    44641 agcatcaggc gcagttgccg cgcgttgcga tagctcatgc ggtgcagggc ggcctcggcg
    44701 cggtcgatgc gcgcctcggc ctcgcgcgaa tcgtcgccgg cgaacaatgc cgcgggatcc
    44761 ggcgtctcgc cctgcagcgg tgcctcgagc agcagcgcct ccagcgtcgg gaagtaacgg
    44821 taggctgtcg cgcgcgagac catcgcggcc gccgcgatct cgtccatgcc gggcgtgcgc
    44881 ccgtcctcca gcagcctggc ggcggcgacg agcaggtcct tgcgtgtgcg caggcgctgg
    44941 ttggcgcggc gctcgcccgg aggtctgttc atggtcgatc agaccggtgc cagctgctgc
    45001 tggatcgcgg cgagatctac ccagacgttc tcgcgcccga tgcgcccggc gggggtgaac
    45061 tcgatcaggt gcagcagccg gaactcgagc gcccgaccgt gtccctcgac gccgaagggg
    45121 cgtccggccg cggtgccgcg ccagagcgac tcatcgacca ggaagttctc gccatacagg
    45181 cgcttgacgc attgcacctg gccgtcggcg aggtcggcga agagccgttc gtagaagggg
    45241 cgcgcattgt cgggcccgaa ggtggggccg ccgggccagc cgacgatatc gtggatcacg
    45301 tccggggcca gcgtggcgac cacgccgtcg atatcgtccg cggcctcgta gccgaaatgt
    45361 tcgtcgatgg cccggtccat ctgcagtgtg gtgagtgtca tggcggtatc ctcgatagga
    45421 gattttgggc tcacagtgag acgatagtct catgaatatc gaaagtccag cccccacgat
    45481 ggaccgcccg acgccgcgtc aaccggcgtc ccggcttgcc cgcaggcctg gcgcgcaacg
    45541 ccgcgcgcac gccgcgcagg atgcatccac cggggcgata tcgctgtgta aagccgcggg
    45601 acggcgccgc ccgcgacgcg cgcgatcggg cacaataccg gttgtcgtgg aaccagcgga
    45661 gcgcgcacgt gaataccagg aaggccgggt tgctgtcgct gatcgtgctg cttctcgcgc
    45721 tgctgttcgc cctcgacctc gatcgcctgc tgacgctcga aggcatcaag gccagccggg
    45781 ccgggcttgc gctgctgctg gagcgctcgt ggccgttggt cgcggcgggc tatttcctgc
    45841 tctacgtgct gatcgccgcg gtgtcatttc ccggcgcggc ggtgatgagc ctcgcggggg
    45901 gagcgctgtt cggcctggcg tggggcactc tgctggtgtc ctttgcgtct tccatcggtg
    45961 ggacgctcgc gttcctggtc gcgcgctacc tgttgcgcga tgcggtgcag cggcgcttcg
    46021 ccgggtggtt gcgcgccgtc gacgagggct tcgcgcgcga tggcgcgttc tacctgttca
    46081 cgctgcggct gctgccgctg ccgttcttcc tggtcaacct ggtgatgggg ctgacgccta
    46141 tccgcgtgcg caccttctac tgggtcagcc agctggcgat gctcggcgcc acgctggtgt
    46201 acgtgaacgc aggcacgcaa ctcgcgcgcg tcgaggcgct gtccgatatc gtctcgccct
    46261 cgctgctgct gtccttcgcg ctgctgggcg tgttccccct gattgcccgg cgcgtgctcg
    46321 cggcgctgca ggcgcgccgg gcgtacgcga aatggcagcg cccgcgtcgc ttcgagcgca
    46381 acctggtggt gatcggcgcc ggcgcggcgg ggctggtcag cgcctacatc ggggccacgg
    46441 tgaaggccag cgtcacgctg atcgaggcgc ggcgcatggg cggggactgc ctcaacaccg
    46501 gttgcgtgcc gagcaaggcg ctgatccgca gcgcgcggct cgcgcacgag atgcgccatg
    46561 ccgggcggtt cgggctggag gcgctcgagc cgcgcgtcga gttcaggcgc gtgatcgccc
    46621 gggtgcagga ggtggtgcgc gcgatcgcgc cgcacgacag cgtggagcgc tacagcgcgc
    46681 tgggtgtcga ggtgctcgag gggcacgcgc gcatcgtcga tccgtggacc gtggaggtgc
    46741 attccggcga cggccggaca cgccgcctca cgacacgcgc gatcgttatc gccgcgggcg
    46801 ccgaaccgta cgtgccgccg ctgccgggac tggacgaagt gggctatctg accagcgaca
    46861 cgctgtggga tgcgctggcc gcgatggagc ggccaccgca gcgcctcgtg gtgctcggcg
    46921 gcggcccgat cggctgcgag ctggcgcagt gcctgcagcg cctcggttcg caggtgacgc
    46981 aggtcgagct gatggagcgc ttgctggcac gcgaggatcc cgaggtcgcg cacggcgtgc
    47041 aggacgcgct cgcgcgcgac ggcgtcgagg tgctgtgcgg ctggcgcgcg gtgcattgcg
    47101 agcgcgtcgg cgagcacagg ttcctcgtcg tcgagcgcga gggcgtgcag cgtcgcctcg
    47161 gcttcgacgc gctgctctgc gcggtggggc gcaaggcgcg cctcggcggc tatggcctcg
    47221 aggaactggg cgtgcctgtc gccggcaccg tggtggtgaa cgaatacctc gagacgctgt
    47281 acccgaacat ctatgccgcc ggcgacgtcg cgggaccgtt ccagttcacg cacgccgcgg
    47341 cgcaccaggc gtggtacgcc gcggtcaacg cgctgttcgg cgcggcgtgg aagttccgcg
    47401 ccgactaccg cgtgatcccg gctaccacct tcaccgatcc cgaggtcgcg cgcgtgggcc
    47461 tgaacgagca agacgcgcag gcgcgcggta tcgcgtacga ggtgacgcgc tatggcatcg
    47521 acgacctcga ccgtgccatc gccgacggcg cggccgaggg tttcgtgaag gtgctgacgg
    47581 tgccgggcaa ggaccgcatc ctcggtgcca cgatagtcgg cgcgcacgcg ggcgaactgc
    47641 tcgcggagtt cgtgctcgcg atgcgccacg ggctcgggct gaacaagatc ctcggcacca
    47701 tccacagcta cccgacgctg gccgaggcca acaagtacgc ggccggcgaa tggaagcgcg
    47761 cgcacgcgcc cgggcgcctg ctgcgctggg tcgaacgcta ccatgcctgg cgccgcgggt
    47821 aggcccccgg gtcgccgttc atggcggcaa aagcgtttgg tcacgcactc g
//
LOCUS       DAORUQ010000004        42236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_542_length_42236_cov_6.111994, whole genome shotgun sequence.
ACCESSION   DAORUQ010000004 DAORUQ010000000
VERSION     DAORUQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 42236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42236
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_42236_cov_6.111994"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /gene="arsJ"
                     /locus_tag="PLW13_00780"
     CDS             <1..1149
                     /gene="arsJ"
                     /locus_tag="PLW13_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017337869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HQY68931.1"
                     /translation="LTDGALRMLVVLHFHALGYQPLEIALLFLFYEFFGIVTNLVGGW
                     LGAHLGLNRTMHIGLGLQIVALAMLLVPAAALTVPWVMAAQALSGIAKDLNKMSAKSA
                     VKLLVPAGAEGALYRWVAALTGSKNALKGAGFFLGGALLELAGFRGAVAAMAAALALV
                     WIGSVLTLRADLGKAKNKPKFRDIFSRSRAVNVLAAARVFLFGARDVWFVVALPVYLA
                     SAFGWDHARVGGFLALWIIGYGVVQALAPRITRTRADGRDAFGWAAALALAPALIALA
                     LGADLAPQASLLGGLLLFGALFAVNSALHSYLIVSYACEDGVSLDVGFYYMANALGRL
                     VGTVLSGWVFQVSGLVACLWISAAFVAAAALISVALPRHRPEALRGVG"
     gene            1204..2223
                     /gene="arsB"
                     /locus_tag="PLW13_00785"
     CDS             1204..2223
                     /gene="arsB"
                     /locus_tag="PLW13_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQY68932.1"
                     /translation="MGLFERYLTVWVALGIGAGVGLGLLAPAAFQSIATLEIAHVNLV
                     VAVFIWVMIYPMMIQIDWSAVKDVGRKPRGLLLTLTVNWLIKPFTMAALGVLFFKYLF
                     APWVDPQSASEYIAGMILLGVAPCTAMVFVWSQLVKGDANYTLVQVSVNDLIMVFAFA
                     PIAAFLLGVTQITVPWETLVLSTVLYVVLPLLAGMATRHLLSRRSTHAVGDFVARLKP
                     WSVIGLIATVVLLFGFQARTIVAQPLVIALIAVPLIVQSYGIFGIGLIGARLLKLPHD
                     IAGPACLIGTSNFFELAVAVAISLFGLNSGAALATVVGVLVEVPVMLSLVALVNASGG
                     WYERS"
     gene            2210..5059
                     /locus_tag="PLW13_00790"
     CDS             2210..5059
                     /locus_tag="PLW13_00790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQY68933.1"
                     /translation="MSAPEPRDALADPSLLAPDAAAAALATDPERGLSAAEAARRLLR
                     DGRNELRTVPPDPLWRRILAHFRDPLIYLLLAAVAITLVAWRLEGMPGLPVDAIVILA
                     IVVLNAALGFVQQARSESAAAALAKMTAVTSSVLRDGKLLRVPSAELVRGDLLVLGEG
                     DSIGADARLLQANALRVLEASLTGESVAVLKDTATLAAPAALGDRSNMVFKGTAVAQG
                     TGLAIVTATGMATQMGTIASLLDATVEEPTPLQEEVRRIGRMLGFAVIAIAVVVVGTV
                     LALAEIRGPADVVAVLLLGVSLAVAAVPEGLPAILSVVLAIGVQRMTKHRAIVKKLSS
                     VETLGSASVICTDKTGTLTRSEMTIERAVTGSGSTRITGIGYTPHGRVEHEGRELTAG
                     ALHSEHVVLLSGGSLAGNADLRQDSEGAWEIHGDPTEAAFLVAERKLNLQEWRGRRFE
                     RIAEIPFTSERKMMSTLAIDHEDGDRLLLISKGAPDVLLGRCTHARLGMDIVPLDESL
                     RAAALAAVDRLTDEALRTLAVAYRPLGPGEDARAGEALEHGLVFVGTVGIIDPPREEA
                     APAIREAQRAGIRVIMITGDHPRTAARIAADLGIVAPGTRALSGAELDALDDTAFAQV
                     VRSTSVYARVAPAHKLRIVDALQADGNVVAMTGDGVNDAPALKSADIGVAMGITGTEV
                     TREAARMVLADDNFATIVAAVREGRGIFANIRKFLRYLLSSNMGEVLTVFLGVIGASL
                     IGLQGSGGELALPLLATQILWINLITDSGPALAMGVDPQSDDVMARPPRRLKDRVIDA
                     QMWSGVLQVGLVMALATLFCIDLHLPGGLVEGSHDLDTARTAGFTTLVFAQLFNCFNA
                     RSESASAFHGLFHNPWLWGSVALAVLLQVAVVHLPAGNIAFGTSPLAPDEWLACAALA
                     SAVLWYGEARKLVIRGAERGRRNRETGLTEATHHG"
     gene            5052..6500
                     /locus_tag="PLW13_00795"
     CDS             5052..6500
                     /locus_tag="PLW13_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQY68934.1"
                     /translation="MADLLHLLNGGLGNLAAYLAAHVLLCLLPAFFIAGAMTALIPRQ
                     SITRWLGRGTPGHISYPAAAAAGSLLAVCSCTIVPLFAGVYRKGAGIGPATTFLFFAP
                     AGNIMAIAYTGSVLGMEFAAARFLLCLLFGIGIGLLMALLFWRDDLRHDRQTDALFAE
                     QARIDPVAMRLLLALLLLLVAGTLKLWPLSATLASVQLPLSWVEPWQDTLLRWVPFDA
                     AKGEEGVSFQGSVLIALLVLIAVSAKQGLDDIVEGANRWTWVALGLTAATLLVATLGL
                     RPRDGGLALEITGRFCAVAAALLAISHYGRRLDAQLWRSWLWESWRFVKQIFPLLIIG
                     VFVVGVARQLIRPEWIESLAGQNDLFANAVAVLFGVFMYFPSLVEVPVARMFLDLGMH
                     PGPLLAYLMADPELSLQSMLMVAAVIGRPKTAAYVGWVALFSIAAGLIYGAWIDGANR
                     WFLVLGTLGFVAALALTVNRLHERVTPGSHAT"
     gene            6539..6784
                     /locus_tag="PLW13_00800"
     CDS             6539..6784
                     /locus_tag="PLW13_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY68935.1"
                     /translation="MLTLKVLGPGCANCRKLEAVAREAASGSGIEVQVLKVSAMKDIM
                     AYDLIATPGLVINEKLVSSGRIPTQAEIREWLEAAGA"
     gene            complement(7273..7716)
                     /locus_tag="PLW13_00805"
     CDS             complement(7273..7716)
                     /locus_tag="PLW13_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68936.1"
                     /translation="MSTPTFAQLKNWADNYVKLWNAGDRAGWAANWRAVAPGEFSMLD
                     PVGTPMKHGFQHCALDSYDLFQPTVRFRVHPGSLFICGNEVAWLLENHFKEGGEERFA
                     YSIETYRFEPDGSVVIRTYYKVPAHAAGDLGKLFETYLPDSPAGA"
     gene            complement(7753..8529)
                     /locus_tag="PLW13_00810"
     CDS             complement(7753..8529)
                     /locus_tag="PLW13_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY68937.1"
                     /translation="MSEFETVRYAVDEGVALITLNRPAVMNAFNGQMRRELLAAVRCA
                     SDDAAVRVVVLTGEGRGFSAGADLADGVPPLVQQQIDEEYKPVLMAISEAPKPFISAV
                     NGAAAGIGSSFAMACDLTVMADNAYIYQAFMAIALVPDGGATWHLVRQLGRKRAFEVI
                     VEGEKMGAARCLELGLANRVVPAERLLEETLAWARTIAKKAPLALRYSKEALNKAMQL
                     DLADTISYEAKLQNITFISEDTREGVQAFFEKRPPVFKGH"
     gene            complement(8593..9480)
                     /locus_tag="PLW13_00815"
     CDS             complement(8593..9480)
                     /locus_tag="PLW13_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY68938.1"
                     /translation="MPLSAHILYFTTVLIWGSTWLAIKFQLGDIDPLVSVIYRFGLAS
                     MLLFAWCVLARARLRLAPREHFFIALQGACLFGLNYWLVYWSEVYLTSGVVAVIFATM
                     VFMNFFNAALFLKRPLVPVVLFGGLIGIGGVALLFWPEFGRLSIDDTALRGLLLALAS
                     TYVASLGSILATRNAVFGLKVITVNAWGMFYGTALLTLAALVTGAEFRFPAGLPYTAS
                     LLYLSVFGSIVAFGAFLRLIALIGPERAGYNAMMTPVVALLLSTLFEGYQWTLPAVAG
                     LVLIVLGNALVLRRPAGSS"
     gene            9771..10154
                     /locus_tag="PLW13_00820"
     CDS             9771..10154
                     /locus_tag="PLW13_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HQY68939.1"
                     /translation="MGKQRSDQPGPAAPATPNPFAALASLRGTLPEGTGGEPAAASAG
                     DSPFAAKLVIARERKGRGGKTATLVRGVALTGAALESFAREMRQALGTGGNVEGDAIV
                     LAGDQSARAAQWLRERGATRIVIGN"
     gene            10158..10550
                     /locus_tag="PLW13_00825"
     CDS             10158..10550
                     /locus_tag="PLW13_00825"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQY68940.1"
                     /translation="MAHATGYALFDTAIGRCAIAWGARDDLRDLVLDLDDITEFHRRV
                     FATARAIAPGNTITYGELARRLGTPGAARAVGQALGANPFPIVVPCHRIVAADGSLGG
                     FSAPGGARTKRRMLQIEGAIAQADIGFE"
     gene            complement(10573..11247)
                     /locus_tag="PLW13_00830"
     CDS             complement(10573..11247)
                     /locus_tag="PLW13_00830"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQY68941.1"
                     /translation="MRRGLGAARGHGLAVALMICVAGLIAASDALFASSAQLIAFLEA
                     EIVARPLAGQLLFVLLAMLSAMLAFFSSALLVPVGLSAWGPLASSALLWVGWLLGGIA
                     SFAVGRFLGDTLAPGLLGSRRIASFREQLNARTRFLHVLLLQAALPSEIPGYVLGMAR
                     YPFHRYLAALALTELPYALGTIYLGQSFLERRSVMLLALGVLAIALSTTFYRLFREGI
                     GARRGN"
     gene            complement(11244..13019)
                     /locus_tag="PLW13_00835"
     CDS             complement(11244..13019)
                     /locus_tag="PLW13_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQY68942.1"
                     /translation="MDWQGWFTVALTVAVLATLITIPRLTTDLVLMGALLLLSITGIL
                     QPDEALSGFANTGLMTVAAMFIVAAAIRASGGVDLVVERVLGRPAGPRGALVRLMLPV
                     VGLSAFLNNTPVVATMIPAVSQWARRIKVPSSALMIPLSYASILGGTITLIGTSTNLV
                     VNGQYQVLTGKPGFALFDIAPIGLIAALVGTLFVVFAAPKLLKVRRSPDQTFADTRSF
                     TFEVAVARDGPLLGKTIQQAGLRHLERIYLAEIERDGSVITAVAPEEMLRGGDRLVFV
                     GETEAIVDVLRINGLVAAVGSQPALERNVPERRLVEAVVAEHCEVVGHTIRDGRFRDR
                     YGAVVLAVARNGEHIRGRLSSIILEPGDLLLLEARPAVVGRLKQSRDFLLMNVLEHEL
                     PDHSHALISWAILGAIIALATTGLMSMLNAALLGVAAMLATRCIGVNEARRSLDLPVL
                     VTIAASFALGNALEKTGAAEYLGNGIVAAAGNDAWLLLALTYLSVMVLTELITNNAAA
                     MLMLPVVLAITEAAGLHDVPYVMAVMMAASASFATPIGYQCNLMVYGPGGYRFSDFLR
                     IGVPMNLLMALVTIAAIPFIWPLQR"
     gene            complement(13192..13944)
                     /locus_tag="PLW13_00840"
     CDS             complement(13192..13944)
                     /locus_tag="PLW13_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68943.1"
                     /translation="MMKQNFLAAAVAALALQAAMPAAAEDADKCCFRLTPYYWAVGID
                     GNLEINDEQGSTDIDFNQDIGDVTDNLEFNGSLMLEHNKGRWSNFAAVDYIKTENDDT
                     DINVAGSEIEVEADTLLATAATGYRFGAGGDSWVDLLVGVRYGKLDLEFNEKNGPGSF
                     NADDDLVDGIVMLRPRVALGRNWAFSPTASIGAGDSDLVWELAPELVYTNNCCNLEFR
                     FGYRTVNYEIEEGDAEADFSFQGPMVGLGFAF"
     gene            complement(14104..14847)
                     /locus_tag="PLW13_00845"
     CDS             complement(14104..14847)
                     /locus_tag="PLW13_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HQY68944.1"
                     /translation="MSETVTACPRTLEISVLRHNPSDPVSTPRLERFEIEEADGMTLF
                     IALNEIRENQDPTLQFDFVCRAGICGACGMLIDGRPGLACRTLTRDLGSRVTLAPLPV
                     FRLIGDLSVDTGEWMQGMAERLETWVHPREQEPDLGALEERMEPALAEQIYELERCIE
                     CGCCVAACATARMREDFVGAAGLNKIARFRLDPRDARSDAQFYELIGDESGVFGCMTL
                     LGCEDNCPKNLTLSTQIAFLRRKMLIAAS"
     gene            complement(14844..16826)
                     /locus_tag="PLW13_00850"
     CDS             complement(14844..16826)
                     /locus_tag="PLW13_00850"
                     /inference="COORDINATES: protein motif:HMM:NF006383.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HQY68945.1"
                     /translation="MRTIQTDVLVVGGGLAGLRTAIGVRRRGFEAIVLSLVPAKRSHS
                     AAAQGGMQASLGNAAMGEGDNEDVHFADTVRGSDWGADQDVVRMFVNTAPKAVRELAA
                     WGVPWNRVQRGEHEVVMNGKRVTLTERDEAHGLITARDFGGTRKWRTCYVADGTGHSM
                     LFTMSDQAVANSIPVHERMEALSLIHDGARCHGVIARNLATGELVAYAARATCIATGG
                     FGRIYRVSTNAIINEGMGAAIALETGVAALGNMEAVQFHPTAIFPAGILVTEGCRGDG
                     GLLLDGREHRFMPDYEPDKAELASRDVVSRRMEEHIAAGHGARSRFGEHLWLDIRLLG
                     RRHIETRLREVKEICNYFLGIDPAEQLIPVRPAQHYSMGGIRTDHRGESRTLKGLFAA
                     GEAACWDMHGFNRLGGNSVAETVVAGMIVGEFIADHCESPDGAADVPMALVREFHARE
                     SRQLAEHAARGGPESPFALLREMQVAMTDNVGIFRSGERLAEAVTQLQQLLVRSRDLG
                     LRSQARGANPELVAAYRLPRMLKLALCVAQGALARTESRGAHFRSDFPVRNDRDWLAR
                     TLATWPDPDATLPVLAYEPLDVRRMELPPGWRGYGGKDYIDHPDTPARQAEVDRVRAA
                     TAGAGRLAQQQALMPWEHQLPPPLRGRNARLGDHDT"
     gene            complement(16826..17599)
                     /locus_tag="PLW13_00855"
     CDS             complement(16826..17599)
                     /locus_tag="PLW13_00855"
                     /inference="COORDINATES: protein motif:HMM:NF010072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HQY68946.1"
                     /translation="MDNPTSRAIARSRIDRAPAWLDLVQGASGLALALFMWGHMLLVS
                     SILLGKDAMYHVARFFEGVYFFGRPYPQLVTAIAGAIFLLMIVHAVAAMRRMPSSYRE
                     YRTLFRHSRDLRHADTWLWLMQVATGLVLMFLAGVHLYTMMTHPGDIGPYESADRFVS
                     GRMWPLYLVLLFAVELHGGIGLYRLVLKWGLLPRSADPRAQRRRLVRLKWAITGFFLV
                     LGLLTFAAYMKIGIEHRDRAGERYVPVALREAVPQAGGG"
     gene            complement(17763..19616)
                     /locus_tag="PLW13_00860"
     CDS             complement(17763..19616)
                     /locus_tag="PLW13_00860"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQY68947.1"
                     /translation="MNTATPAACANYRPTSTMRQHIHLAPRRMSWDFSGVPACPVEDD
                     PNLAAFILAISYFVVAFEDFGIPVVRNHLAKLAGYPELAREAQAFITQEALHSQGHVL
                     LNTVMNERHGIDTTVIEREFRRFIHDMSAGAQQDQLAAVAALEHVIFSIAEWYETTDE
                     LRRRVHPEVDRLVLWHCMEETEHTAVVYDVYEYIYGAQPDAYSRRMAALTRGTRCGVR
                     ALVRMWRALVPQMAQRMGCSAASVMPWVHLATEGSRYLVDFLRFLSPGFTPWRDAATA
                     GLLPSLRERMSPRRLRPDGLHAVRVASVREVSADARTYELVAPDAAQLPPWTPGAHID
                     VEIETGIVRHYSLCGDPADRGCYRIAVKREDRGRGGSLRMHQEIRSGDTLRISLPRNL
                     FALQQQPADTNTLLLAGGIGITPLLAMAYSLHAAGADFELHVCSRDRQFLPFADDIVR
                     WPFSASVRTHLDTQAAGGRVDLGRLIPRWDAGKPRALYVCGPAPFIDAALGVAQQAGW
                     PAEALHCERFAHDAAGSKPDDAPFTLHLRRSDITLNVPADRSILEVMEARGMKPASSC
                     REGICGSCICTVVEGEVDHRDAVLTALEHRQTQRIAICVSRARGERLAIDL"
     gene            complement(19698..20312)
                     /locus_tag="PLW13_00865"
     CDS             complement(19698..20312)
                     /locus_tag="PLW13_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HQY68948.1"
                     /translation="MDLLKRAKQMKFPCIALVAVLAASAVAPGHAAEQAGAQPVKVDM
                     PGVVNFSRIDHSSGFAGSTAGFGGATQPSAMSSLRGAGFATVIGLRFASEAGAEIEAG
                     RVAAEAAGLKYIHLPFDPKNPGPKTPDTEVIGEFLDALGHEANQPVYIHCNSATRVAA
                     LWMIGRVLVDGWEIDAAGKEAALIAAKPEEAIAFATRYIESRRQ"
     gene            complement(20327..21790)
                     /locus_tag="PLW13_00870"
     CDS             complement(20327..21790)
                     /locus_tag="PLW13_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68949.1"
                     /translation="MINTCRSPVAGLAGPGAGADGRAFCQFKPIDRRFPRAGATAGHV
                     RVAHTDSIEGERGVDEIKKGSSGLSRRNFVASSGVLAAGGLALGAGLAPRATAETLSG
                     GTSTKGRTVPLNWKEVYFTNCPMVSANNIDQELGWCKTDFKAIGIDYSYFRSRRENDW
                     YPHYIHNLDNLIRFGGLYPPIHVHADVRRTCLLGATWVYEGGCMAVRADDNLWRMEDL
                     RGKKIGLSKSLNTIKNDWWRIQEHMGIQNMLMMNDMSMKDVQIVEFPYADDWYDDPKM
                     LAPMINPTDLWATRDHKRDLAFRPLESALLSGKVDAIYTQSKVFQHLQEDTGEIRMIE
                     DLSRYPDWKLQVANTPAVITCTDVMAREHPELVVTYMKSMIKVGRWANEFKQAAAVIL
                     DRQTFYRDAEDTYRGIKDVDMVPNLSHQNLSCIETGKDFMLKNGYIKNDFDVHAWAAP
                     EFLEMAAKELLEEAWKKKTTEMLPEPSELQLESRRMG"
     gene            21789..22829
                     /locus_tag="PLW13_00875"
     CDS             21789..22829
                     /locus_tag="PLW13_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY68950.1"
                     /translation="MRTSRPSAGETVHPVYIRFVCTLLRQNGKDPEAVLAAVGLDSHR
                     VTASSDWLEFDTFERFLEAAGTALGCPSFGLDLGSRFQLVAYSAVGHAAVTSATLRDS
                     LQVLSRFGALRLRNLSVRYLEETDGATVVMYLRCHSESARRILYEVLLALLVRLTESV
                     TGSDADYLDVQMPFASPPWVGRYAMFRFARLGFGGEQLVVRYPRAALDTACLSADAKL
                     CERATRECEEQLHLAETTPLSTRVRELVSRSLEDTRVAACPEITDIADHLHVSARTLM
                     RRLKREGSSYQAIVDEIRAEQAIWLLKYSSMPIAELAARLGFEDTSSFNRAFRRWLGV
                     APTSLRRGRRSG"
     gene            complement(22872..23894)
                     /locus_tag="PLW13_00880"
     CDS             complement(22872..23894)
                     /locus_tag="PLW13_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY68951.1"
                     /translation="MPKPKQSLIEPTTVAAYVEAITRALQARGADPDEVLREIDVLRI
                     HGNDPLVRITDSTVNAIYARAVALTGDEYFGLRVAEHIVPGTLHALGYALLASETLED
                     FCHRFVRYYGLVSQSAELRVFVEGQTFRLEADPRNPDICVETQDVWAALVLRLMRMAY
                     RADFCSRALDLRRPVPAGGEEPFRAFFGCPVRFGQPHLCFHFELAAMREPLLGANREL
                     AQHNDQIAMTYLEKLDRDDIANRVRTQLVNGLNAGIFSRAEIATRMHMSPSTLQAKLA
                     RQGVSFLQVLDDTRQQLALGYVRQKRLSITEIAFMLGFSDVSNFNRAFRRWTGKAPSE
                     YRVSAT"
     gene            complement(24003..24842)
                     /locus_tag="PLW13_00885"
     CDS             complement(24003..24842)
                     /locus_tag="PLW13_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY68952.1"
                     /translation="MITGAEYRRLPINGCEMNVLLAGEGEPVLLVHGFPDDHTVWREQ
                     VQALVAAGYRVIAPDTRGCGESGIPGRVADYRVEILVEDLRALLDALGIDAVRLVAHD
                     WGAVIGWRFVLAHPQRVDRFVALSVGHPTAYARGGLVQKLKGYYVLLMQLRGIMEWLA
                     MCGDWFLFRQLTRYAAEFPRWRERLSRPGRLTAGMNYYRANLGALFAGELPSARVPVF
                     GVWSSGDLFLVEGQMIASERYVRGPWRYARIDGANHWLQVDAPARLNPLLLEYLGLPR
                     ASL"
     gene            complement(24886..26313)
                     /locus_tag="PLW13_00890"
     CDS             complement(24886..26313)
                     /locus_tag="PLW13_00890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY68953.1"
                     /translation="MSQAVLPLNQIAFAVLDLRRTEAWWREGLGFLPAGGTRALFRGP
                     LSGRIQKLPGSACTCWCLLGRNDWCQLELFQYEKPASQLMPADYRTSDLGYSRCGVWV
                     ADFDAALARLARLGSVPLAAPVGVPGRRRACVRNPDGVYVELMEDDPLPAQNARGRQD
                     CPVAIRSATLSTPDMDASVDFLTRGLGMREVPRQLHEDAHEALWGLAGARCERRVFTD
                     ASGDPAMLLELVRYRDPPGRPFPPGYRLCDQRILNVCFGDPRGSAGVMALYRRALGAG
                     ARHNCAPLNLGITGCVYVSDPLGFSWEIMWARPGLAQRAFGFVPLPRAQRPQLDNQRV
                     EASLRIEAPAAEVFAVLGDHRGMSDWAGFGEYRLVRRGDGELGGRVAERLLESPLGTI
                     REQITDWWPGRGYRYRIIAGSPFIGYWGEVGLVPEGDGTRVEWILRFRSRIPGLGGLF
                     RVLLRRKLRAALQGLRQQVSRARNP"
     gene            complement(26310..27509)
                     /locus_tag="PLW13_00895"
     CDS             complement(26310..27509)
                     /locus_tag="PLW13_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQY68954.1"
                     /translation="MNLKAVVCQNTGLRVADLPEPEPGRGQVLVQVLHCGICGSDLHV
                     REHCGHMKQLMARVAFDAQFPGVGDAVVFGHEFCCEVLDHGPGCERRIRPGTRVVAQP
                     LLRVGNGIDMLGLSVRSPGAYAERMLLQESALVPVPSGLSSEMAALTEPMAVAWHAVR
                     RSEIARKDVAIVIGCGPVGLAVICLLKARGVKTVVASDFSPGRRALAQRCGADVVIDP
                     ATTSPWSNWQEYGFMGGLGDLLELALGTREKLAKLPLLWWHVWRLAEALGQNPARPVI
                     FECVGVPGLLQQLIDGAPLLSRVVVAGVCMQVDRIEPAVAIHKEIDLRFVFGHSPLEF
                     RDTLHLIADGKVDCAPLITGVVGLDGVANAFDALKDPERHAKILIDPASAATRPLASA
                     PARAHAP"
     gene            complement(27635..29458)
                     /locus_tag="PLW13_00900"
     CDS             complement(27635..29458)
                     /locus_tag="PLW13_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQY68955.1"
                     /translation="MKFLHWFGLMALVLGTGAAAGTLDGSVRTSDGKAVAGAMVTVWN
                     AARDRKETVYSDAAGRYSLPTGFAGTLQVRARTPYFRDVVQTVELGAGQQLAVDFSVE
                     KIASVEELSDTLTASAHAAMLPFQDGEAKETFISQCSFCHQQGNALTRRPRSESEWTD
                     TVRRMEGYLAMLTWREQRNIAAMLARGFTGKPVAAIQEHDFSPEQAKARIEEWHAATP
                     MSFLHDTIVLADDRLMGIDEGRDEVYILDRHSGQIEKYEMPVTDEVVGGNFRGMQLPI
                     GIFTGRHGPHSGAQISDGRIFFTGALSSNLLMFNPATKEWKLYPIPQGFLWRKGLYSH
                     TIRADKDDNLWFTVLVSNTVMKFDPKTERFTEVATPHNGVMRWISDTFIGVVLKICSW
                     FPEKNYHLYLSHHKWMNGGRDVFNWPYGIDIDPRDGGIWYSKLLSNKIGHIDPKTLAV
                     TEYDVPHGGPRRLRFGPDGSLWIPSFDEGYLMRFDTRSKNFDSIALPLLAPNEFELPY
                     ALNVDRNGDVWIATNNSDRVMRYIPAERKFVAYPMPNRVIWFRDFEFTQDGRVCASNS
                     NLPAHAHEDKLPAFVCIQPDATDTTSLLPAHQRHHLRATPP"
     gene            complement(29530..30225)
                     /locus_tag="PLW13_00905"
     CDS             complement(29530..30225)
                     /locus_tag="PLW13_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68956.1"
                     /translation="MKHFRFEIVLAVALLALFLAGSAWFAPGSRLSQAEVDRHLTMLE
                     QHAPWPAEEKRAILEHLRAWGEADDGRPVYMLNLMRYYPELRPMPGVEGFKGTPEQAN
                     ALYEQKVMPLLFKLGAYPLFGSAMQGVPAGAQSSTNLIGLDPAIDNWSRVLVVRYPSR
                     RAFLELIGDPAYLEYLPYKAAALMVALTPMKGDLVLPDALPALALLLLTVFFAAAWIR
                     ALARARAAARQCD"
     gene            complement(30281..32632)
                     /locus_tag="PLW13_00910"
     CDS             complement(30281..32632)
                     /locus_tag="PLW13_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY68957.1"
                     /translation="MRTTARILSARKLLALVTVTLAAAPLGALAQAQLEEIVVTAQKR
                     EQSLQDVPMAVSAVSGQAMQKAGISDIKDLARQVPSLQVQSNTSPLAMSYRVRRVGNL
                     GNIPTFEPAVGVFQDGAFRNRPVFSAGDMFDVDRIEVLRGPQSTLYGKNTTAGVVAVY
                     SRKPSEELTGNAEFTAGNLEGASDAGMYRFVGGVSGPLADTLSASLGLSYSTNDHVSE
                     SALSVSDEDANDLERGSVRGQLQWQPSDTLSVRLIGGALQEDDDQFTSDLYIEPGSGA
                     AQAGAFLRGAGLAEACASNDATDLEHCSRKAVTSDVDAREATLLVDYALANDWTVTSI
                     SSWDWFEFKGTNDDVAQLASPLLRFHDTQEGESWQQELRLASAGGETVDWLTGVFWYQ
                     NEFERGDDGDRPIFLEDTYSAHPSSARLLQLLLRLPIPVPFATPGQNGLLDASQDTDY
                     VGLFGQATWNITDRFSVTGGARWQTEEKDAEVLQSLTVPGLSLLSANLISTAPANNGE
                     MDRDTDEVTWSLTPQFTLSDDVNLFATVSHGFKSGGFNVGWGRTPIDQREFKDEDIMH
                     YEAGVKSTLLEGAMQLAVSAFYTEYDDYQDAAFVGQTFNVTNAEKAELKGAEAEGKLL
                     LGEKVSADFGISYADFTYEEYTSGLCYAGRTPDGSLPGTCDLSGEHPVNAPEWTTHVG
                     LLYEADVSWGEVYARADWSWSDEYNTSSQIDPRLVQDAYNWVNLRLGTRWDAYEVVAW
                     VDNAADEEVVNLDASMNLFANDKSYQSFRQAPRSYGLTVRAAF"
     gene            32892..33362
                     /locus_tag="PLW13_00915"
     CDS             32892..33362
                     /locus_tag="PLW13_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68958.1"
                     /translation="MNHLTRSASLLLAIAAATPLYASTLIPAKGQTPEQIQADTAACQ
                     SQATQASQSAAPAPAPQGGRLKGAAVGAAAGAAAAQVRGNQYEAYDRLDSDVQQEYRQ
                     NQAQGAAAAGMVVGGAKQRQQRRQTQQQAAAQSQQQGSQAFDSCMSARGYTVQP"
     gene            complement(33550..33765)
                     /locus_tag="PLW13_00920"
     CDS             complement(33550..33765)
                     /locus_tag="PLW13_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY68959.1"
                     /translation="MTDTSAEHLREAISVIGAQRIMFGSDLRAISSNHSIADNLITAI
                     ETRPTAEEREQIAWKTANQIYKLGLKG"
     gene            complement(33734..34066)
                     /locus_tag="PLW13_00925"
     CDS             complement(33734..34066)
                     /locus_tag="PLW13_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68960.1"
                     /translation="MDYGDEVSKVVRQIAEELQSPAMSATRGVGEIVPITTEIHPAKI
                     TRDMAPIMEVLKARGDLPIMFPTGYTGWKGVHWAEHPRVLRCLPVRGDAQRQRLLRHD
                     RHLGRAPA"
     gene            complement(34053..34493)
                     /locus_tag="PLW13_00930"
     CDS             complement(34053..34493)
                     /locus_tag="PLW13_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HQY68961.1"
                     /translation="MSTSHDEQQAGPAVQKPEGSRSEASRRQFLQLSAAASAAAAGAL
                     VSSAAYADDDPVMAGRSTRPSWAPTKYLIDCHCHVGSGPTVAELAPTIHTARDWGALR
                     TRQPEKFAKAFSEDALDDSAILPDLPARSDFECGRQGDAGSGLW"
     gene            complement(34646..35374)
                     /locus_tag="PLW13_00935"
     CDS             complement(34646..35374)
                     /locus_tag="PLW13_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68962.1"
                     /translation="MRKILGAAVAAIALQGAAAAAAALAADSGFTVTPYLWAVGIDGD
                     IQAHGEDTTVSMDFQDVVENLDMAASALFEANVGDWVSYAMVDYLSLDSGDVDTRYPG
                     IDADLEMESTLATVATGYRVHMGERSTIDVQVGIRYARMDTELWIEGVGQRDADNNVY
                     DGILALRPRLALSRNWTFSPTMSIGAGDSDLTWELSPQFVYDCDCGFDVRVGYRNLNY
                     DFEKGSDSMDLTIKGPVIGFGFSF"
     gene            complement(35448..37274)
                     /locus_tag="PLW13_00940"
     CDS             complement(35448..37274)
                     /locus_tag="PLW13_00940"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY68963.1"
                     /translation="MASSQQTLKRLGAIPRLLLLIAAVAGWCALSLASAAVLAAPPAK
                     RPNIVVILGDDLGYADTGAFGSEIRTPGIDSLANSGVRFTNFYTHASCSPTRSMLLSG
                     VDTHRNGLGNMDEWTAPNQRGVAGYEGHLSHGVATLPELLRDAGYHTYMVGKWHLGKA
                     PDLIPAARGFERDFSLLDGAGSYWDMTNFTAASPQSVFTEDGRYLTRLPDDYYATKTY
                     TDKLIGFIDANRGDGKPFFAYVAHQAPHDPYHLPRDWRNRHAGAYDKGWDAVRQARLK
                     RQVELGIVPAGTQLAEPMWTSPDPELLDPEGRALLARKMELYAGLVENFDHHIGRLVA
                     HLKEIGEYENTVFIVFGDNGAEGSDLFRMITGTPGTPDFKFAQEHWPRRDPADWGEPG
                     SYVAYGPMWAQVSMTPFSQFKGWLGEGGIRNALIVSGPVVKRAPGSINNGRMHVADIM
                     PTLLGIAGAPYPATREGKALPPLIGKSWSAVLAGSADSPRSAQDYMAWELFGNRAVLQ
                     GDWKLRWQYRPFGKEAWELFNLAEDPAERNDLAAAQPEKVRELTALWDHYVKANNVVL
                     PSRSPFEPTDPEHPPPEDPGFPPLKLKRAFVPPADMLAEPKP"
     gene            37481..38533
                     /locus_tag="PLW13_00945"
     CDS             37481..38533
                     /locus_tag="PLW13_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY68964.1"
                     /translation="MSSASPENKAPAAVTIDEICDPTTASAGIELLDQDAVQLQSMPL
                     RVRRVIVRLDEAAVVLHSSNVRVRTHTSIREGFLAYVTFGPQASGTVNGLPIRPGLML
                     AAEPATEVGFVAAPGWESITFLLPPHIMSTQLSARHREAEFRLPRGVEVLQANPEMVR
                     ALHDWGRRLIDTATTTPELFDQRRREMGAVQVELIELLLATIGAADDIASSRSDRTRQ
                     AQSRIVRSAEKYALSHIADHVYVSDLCRAAAVSERTLEGAFKEIMGLGPVAYLIRLRL
                     HRVRQALLGATPGSTTVSATALDWGFWHFGEFSSAYKDCFGELPSDTLRRVPAAGRED
                     TKPSSTRKRRPSRPRG"
     gene            38621..41041
                     /locus_tag="PLW13_00950"
     CDS             38621..41041
                     /locus_tag="PLW13_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY68965.1"
                     /translation="MIISNPYQTRSRHDDARGKRRIAMHPPGRTILAIALAIAAGSAG
                     AQNPGGQLEEIVVTAQKREQNLQDVPMAVSAFSGSALQNAGVADIEDLARQVPALQVQ
                     TNTSAATTNYRMRRVGNLGSIPTFEPAVGVFIDGAFRNRPLFSAGDMFDIERIEILRG
                     PQSTLYGKNTTAGVVAMYTRAPTEEFSGSGEFTAGQIEAAADAASYRFVGGLSGPLTD
                     TLGASLGVSYTSNDHLDESALSVSNDDANDLERMALRGQLQWQPSDALSVRLLAGATD
                     EDDETMTPDTYIAPGGPAATAGGVLRQFGLAETCSSNDPLDRELCARVPNTTDLQSRE
                     ATLLVDYTLGNDWTVTSVSSWDWFKFKGTSDDIVQLASPLLKFHDTQEGESWQQELRL
                     ASAGGETVDWLTGVFWYQNEFERGDGGDRPIYLEDTYSAHPVPSLLLQRLFGTPFPVP
                     FAAPGQNGLLDAQQDTDYLGVFGQATWNLTEQFAITGGLRWQTEEKDASLEQSATAPG
                     ASLITLSLLPAAIGGDMDRDTDELTWSITPQYALTDDVNLFATAAHGFKSGGFNIGWG
                     RTPLVDASGNNPREFDDESVMHYEAGVKSTLLDGAMQLAFSAFYTEYEDYQDAAFIGQ
                     QFSVGNAETAELKGAEAEGKVLLGEHLTADFAISYADFTYDEYSSGLCYPGRVPDNAA
                     TGACDLSGEHPVNAPEWTTHLGLIYETDVSWGDVYARADWAWSDEYNTSFSADPRLVQ
                     DSYDWLNFRLGTHVGENYEIVAWVDNALDEDVVNFDAQLALFGADKSYQTFRQASRSY
                     GLTLRAEF"
     gene            41051..>42236
                     /locus_tag="PLW13_00955"
     CDS             41051..>42236
                     /locus_tag="PLW13_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY68966.1"
                     /translation="MAIIDFFDRGWGLNPDGAAYIQDERRYSFREVGELSCRIANRLL
                     ALGLPKGTKGAVWSVNDVTAWTCTLGLWRANMSWIPVNERNAAQENHYILEAFDCEVL
                     FFQAHFADVIAGLRPRLPGIRHWICIDADAADAPSLERWCAGQPATRPEVAVDLDDVI
                     ALSPTGGTTGMPKGVMNTHRSVQTFFAHFMLACTYRDGATPVNLAAAPMTHTAGLLSV
                     PCTAQGGTVVIVSKPDPALLLQAVVRHRVTEFFLPPTVIYRLLEAPGIETVDFSSVRY
                     LLYGAAPMSVEKLKKAIRLWGPIMAGGYGQTEAMASICNLRPDEHMVDGNLADDTRLS
                     SVGRPNALIRVEILDDANRILPRGETGEICVRGDLVMKGYYKAPEKTAETVVDGWLHT
                     GDI"
BASE COUNT         6821 a        14374 c        14281 g         6760 t
ORIGIN      
        1 ctcaccgacg gcgcgctgcg catgctggtg gttctgcact tccacgcgct cggctaccag
       61 ccgctcgaga tcgcgctgct gttcctgttc tacgagttct tcggcatcgt gaccaacctg
      121 gtcggcggct ggctgggtgc ccatctcggc ctcaaccgta cgatgcacat cgggctcggg
      181 ctgcagatcg tcgcgctcgc gatgctgctg gttcccgcag ccgcactgac ggtgccgtgg
      241 gtgatggcgg cgcaggcgct ctcgggcatc gccaaggatc tcaacaagat gagcgccaag
      301 agcgcggtga agctgctggt gcccgccggc gccgagggcg cgctgtaccg ctgggtggcg
      361 gcgctcaccg gctcgaagaa cgcgctgaag ggcgcgggtt tcttcctcgg cggcgcgctg
      421 ctcgagctcg ccggattccg cggcgccgtg gccgcgatgg cggcggcact ggcgctggtg
      481 tggatcggca gcgtgctgac gctgcgcgcc gatctgggca aggcaaagaa caagccgaag
      541 ttccgcgaca tcttctcgcg cagccgcgcg gtcaacgtgc tggcggcagc gcgggtgttc
      601 ctgttcgggg cgcgcgacgt gtggttcgtg gtggcgctgc cggtgtatct ggcgagcgcc
      661 ttcggctggg accatgcgcg cgtcggcggc ttcctcgcgc tgtggatcat cggctacggc
      721 gtggtgcagg cgctggcgcc gcgcatcacg cgcacccgcg ccgacgggcg cgatgccttc
      781 ggctgggccg cggcgctggc gctcgctccg gcgctgatcg cgctggcgtt gggcgcggat
      841 ctcgcgccgc aggccagcct gctcggcggc ctgctgctct tcggcgcgct gttcgcggtg
      901 aattcggcgc tgcacagcta cctgatcgtg agctacgcgt gcgaggacgg cgtctcgctc
      961 gacgtgggct tctactacat ggcgaatgcg ctcgggcgcc tggtcggcac cgtgctctcg
     1021 ggctgggtgt tccaggtgag cgggctggtg gcctgcctgt ggatctcggc ggcgttcgtg
     1081 gcggcggcgg cactgatctc ggtcgcgctg ccgcggcacc gtcccgaggc gctacgcggt
     1141 gtcggctagc gacagcaatg cgggcgccgg agttgagcgg cacactcacc aggggattgc
     1201 ggtatgggtc tgttcgaacg ctatctgacg gtatgggtcg cgctcggcat cggcgcaggc
     1261 gtcggcctcg gcctgctcgc acccgccgca ttccagtcga tcgcgacgct ggaaatcgcg
     1321 catgtgaacc tggtggtcgc ggtgttcatc tgggtcatga tctatccgat gatgatccag
     1381 atcgactggt cggcagtgaa ggatgtgggt cgcaagccgc gcgggctgct gctcacgctc
     1441 acggtcaact ggctgatcaa gcccttcacg atggccgcac tcggcgtgct gttcttcaag
     1501 tacctcttcg ctccctgggt ggatccgcag tcggccagcg aatacatcgc cggcatgatc
     1561 ctgctcggtg tcgcaccgtg caccgcgatg gtgttcgtct ggagccagtt ggtgaaggga
     1621 gacgcgaact acaccctggt gcaggtgtcg gtgaacgacc tcatcatggt gttcgccttc
     1681 gcgccgatcg cggccttcct gctcggcgtc acgcagatca ccgtgccctg ggaaaccctg
     1741 gtgctgtcga cggtgctcta cgtggtgctg ccgctccttg ccggcatggc gacgcgccac
     1801 ctgctgtcac ggcgatcaac gcatgcggtg ggggatttcg tggcaaggct caagccgtgg
     1861 tcggtgatcg ggctcatcgc caccgtggtg ctgctgttcg ggttccaggc ccggaccatc
     1921 gtggcgcagc cgctggtgat cgcgctgatc gcggtgccgc tcatcgtgca gagctacggc
     1981 atcttcggca tcggcctgat cggtgcacgg ctgctgaagc tgccgcacga catcgcggga
     2041 cctgcctgcc tgatcggcac ctcgaacttc ttcgagctcg cggtcgcggt cgcgatctcg
     2101 ctgttcgggc tgaactccgg cgccgcgctc gcgaccgtgg tcggcgtgct ggtggaagta
     2161 ccggtgatgc tgtcgctggt cgcgctcgtc aacgcgtcgg ggggctggta tgagcgctcc
     2221 tgaaccgcgc gacgcgctcg ccgatccctc gctgctggcg cccgatgccg ctgccgcggc
     2281 gctggcaacg gaccccgagc gcgggctcag cgccgccgag gccgcgcgcc ggctcctgcg
     2341 cgacgggcgc aacgagttgc gcaccgtgcc gcccgacccg ctgtggcggc gcattcttgc
     2401 gcacttccgg gatccgctga tctacctgct gctggccgcg gtcgcgatca ccctggtcgc
     2461 ctggcgcctc gagggcatgc cgggcctgcc cgtggacgcg atcgtgatcc tcgcgatcgt
     2521 ggtgctgaat gcggcgctcg gcttcgtgca gcaggcgcgc tcggaaagcg ccgccgccgc
     2581 tctcgcgaag atgacggctg tcacctcctc ggtgctgcgc gacgggaagt tgctgcgcgt
     2641 gccgagcgcg gaacttgtgc gcggcgacct gctggtgctc ggcgagggcg acagcatcgg
     2701 cgcggacgcg cgcctgctgc aggcaaacgc gctgcgcgtg ctggaagcct cgctcaccgg
     2761 cgagagcgtc gcggtactca aggacaccgc gacgctcgcg gcgcctgccg cgctcggcga
     2821 tcgcagcaac atggtgttca agggcacggc ggtcgcgcag ggcaccgggc tcgccatcgt
     2881 gaccgcaacc ggcatggcga cgcagatggg cacgatcgcg agcctgctgg acgcgacggt
     2941 cgaggaaccg accccgctgc aggaggaggt gcgccggatc gggcgcatgc tcgggttcgc
     3001 ggtgatcgcg atcgccgtgg tcgtggtggg caccgtgctc gcactggcgg aaatacgcgg
     3061 ccccgccgac gtggtcgcgg tgctgttgct cggcgtgagc ctcgccgtgg cggcggtgcc
     3121 cgaaggcctg ccggcaatcc tctcggtggt gctcgcgatc ggcgtgcagc gcatgacgaa
     3181 gcaccgggcc atcgtgaaga agctctcctc ggtggagacg ctgggctcgg cctcggtgat
     3241 ctgcaccgac aagaccggca cgctgacgcg ctcggagatg accatcgagc gtgcggtgac
     3301 cggatcgggc agtacgcgca ttaccggtat cggctacacc ccgcacggac gcgtcgagca
     3361 cgagggccgc gaactcaccg cgggcgcgct gcacagcgag cacgtggtgc tgctgagcgg
     3421 cggcagcctc gcgggcaatg cggacctgcg ccaggatagc gagggcgcct gggagatcca
     3481 cggcgacccc accgaggccg cgttcctcgt ggccgaacgc aagctgaacc tgcaggaatg
     3541 gcgcggccgg cgcttcgagc gcatcgcgga gattcccttc acctccgagc gcaagatgat
     3601 gtcgacgctc gcgatcgacc acgaggacgg cgaccgcctg ctgctgatca gcaagggcgc
     3661 gcccgacgtg ctgctcgggc gctgcaccca tgcgcggctc ggcatggata tcgtgccgct
     3721 cgacgagagc ctgcgcgcgg ccgcgctggc cgcggtggac cggctcacgg acgaggcgct
     3781 gcgcacgctc gcggtggcct accgcccgct cggccccggc gaggacgccc gggccggcga
     3841 ggcgctcgag cacggcctgg tcttcgtggg caccgtgggc atcatcgacc cgccgcgcga
     3901 ggaggccgcg cccgcgatcc gcgaggccca gcgtgccggc atccgcgtga tcatgatcac
     3961 cggcgatcac ccgcgcaccg cggcgcgcat cgcggccgat ctcggtatcg tggcaccggg
     4021 cacgcgcgcg ctcagcgggg cggaactcga cgcgctcgac gataccgcgt tcgcgcaggt
     4081 ggtgcgcagc acctcggtgt acgcgcgcgt cgcgcccgcc cacaagctgc gcatcgtcga
     4141 tgccttgcag gccgacggca acgtggtcgc gatgaccggc gacggcgtca acgacgcacc
     4201 ggcactgaag tccgccgaca tcggtgtcgc gatgggcatc accggcaccg aggtcacccg
     4261 ggaggcggcg cggatggtcc tggccgacga caacttcgcc accatcgtcg ccgcggtgcg
     4321 cgagggccgt ggcatcttcg ccaatatccg caagttcctg cgttacctgc tctcctcgaa
     4381 catgggcgag gtgctgacgg tgttcctcgg cgtgatcgga gcttccctga tcggcctgca
     4441 gggcagcggc ggcgaactgg cgctgccgct gctcgccacc cagatcctgt ggatcaacct
     4501 gatcaccgac tccggccccg cgctcgccat gggcgtggat ccgcagagcg acgacgtgat
     4561 ggcgcgcccg ccgcgccgcc tgaaggatcg cgtcatcgac gcgcagatgt ggtccggcgt
     4621 gctgcaggtc gggctggtga tggcgctcgc gaccctgttc tgcatcgacc tccacctgcc
     4681 cggtggcctc gtcgagggca gccacgacct ggacaccgcg cgcacggcgg gcttcacgac
     4741 gctggtgttc gcgcaactgt tcaactgctt caatgcgcgc tcggagagcg ccagtgcatt
     4801 ccacggcctg ttccacaacc cctggctgtg gggcagcgtg gcgctggcgg tgctgctgca
     4861 ggtggccgtg gtgcacctgc cggcgggcaa catcgctttc ggcacgtcgc cgctggcgcc
     4921 cgatgaatgg ctggcgtgcg ctgcgctggc cagcgcggtg ctctggtacg gcgaggcgcg
     4981 caaactggtg atccgcggcg cggaacgcgg acgccggaac cgggaaaccg gactgacgga
     5041 ggctacgcat catggctgat ctgctgcacc tgctgaacgg cggcctcggc aatctcgccg
     5101 cctacctcgc ggcgcacgtg ctgctgtgcc tgctgccggc gttcttcatc gccggcgcga
     5161 tgacggcgct gattcccagg cagagcatca cgcgctggct cggacgcggc acgccaggac
     5221 acatctccta tccggcggcg gccgcggcgg gttcattgct ggccgtgtgt tcctgcacca
     5281 tcgtgccgct gttcgcgggc gtgtatcgca agggcgccgg catcggtccg gcgacgactt
     5341 tcctgttctt cgcgccggcg ggcaacatca tggcgatcgc ctataccggc agcgtgctgg
     5401 ggatggagtt cgcggccgcg cgcttcctgc tgtgcctgct gttcggtatc ggtatcggcc
     5461 tgctgatggc gctgctgttc tggcgcgacg acctgcgcca cgaccgccag accgatgcgc
     5521 tgttcgccga acaggcccgc atcgatccgg tggccatgcg tctgctgctg gcactgctgc
     5581 tcctgctggt ggccggcacg ctgaagctct ggccgctcag cgccacgctc gcctcggtgc
     5641 agctgcccct gtcgtgggtc gaaccgtggc aggacacact gttgcgctgg gtgcccttcg
     5701 atgcggcgaa aggcgaggaa ggcgtgagct tccagggctc ggtgctgatt gcgttgctgg
     5761 tgctgatcgc ggtgagcgcg aagcaaggcc tggacgacat cgtcgaaggc gcgaaccgct
     5821 ggacgtgggt cgcgctggga ctcaccgccg cgacgctgct ggtggcaacg ctgggactaa
     5881 ggccccgcga cggcgggctg gcgctggaaa tcaccgggcg cttctgcgcg gtggcggcag
     5941 ccctgctcgc gatatctcac tacggcaggc ggctggatgc gcaattgtgg cgctcctggc
     6001 tctgggaatc ctggcgcttc gtgaagcaga tcttcccgct gttgatcatc ggcgtgttcg
     6061 tggtcggcgt ggcccgccag ctcatccgcc ccgagtggat cgagtcgctc gcgggacaga
     6121 acgacctctt cgccaacgcg gtcgccgtgc tgttcggcgt gttcatgtac ttcccgtcac
     6181 tggtggaagt gccggtggcg cgcatgttcc tcgacctcgg catgcacccg ggaccgctgc
     6241 tggcctacct gatggccgac cccgagctca gcctgcagag catgctgatg gtcgctgccg
     6301 tgatcggacg gcccaagacc gcggcctacg tgggctgggt tgcgctgttc agcatcgcgg
     6361 cgggcctgat ctacggggcg tggatcgacg gcgcgaaccg ctggtttctc gtgctgggga
     6421 cgctcggctt cgttgccgcg ctggcgttga cggtcaaccg gctgcatgag cgcgtcactc
     6481 ccgggagcca cgcgacctga cgattgcttc tttcctcacg acgatacgga gaaatcacat
     6541 gctgacgcta aaggtactgg gaccgggctg cgccaactgc aggaagctcg aggccgtggc
     6601 gcgcgaagcc gcgagcggca gcggcatcga ggtgcaggtg ctcaaggtct ccgcgatgaa
     6661 ggacatcatg gcctacgacc tgatcgcgac gccgggactg gtgatcaacg agaaactcgt
     6721 cagcagcggg cgcatcccga cgcaggcgga aatccgcgag tggctggagg cggccggagc
     6781 ctagctctga cgcccgatcg agcgctggtc acggcatgcg gttagccccc gcccgtgcca
     6841 tgctggctct catgcgcgga cacgccgtga atacgtccat gtaggctcga ctgcggcatc
     6901 catgccgcag acggtcctcg caagagaacc agcatggcac gtgctgcctt ggtggtacgt
     6961 gctcttcatg gtttgagcgc catttcgacg aaactgaaca ccgacgatga accaccaacg
     7021 gcaggcgtga cgaggggcgg gcctggtccg cgagcggacc tgtggatgcc gaaatcgccg
     7081 ggagcgattt cggcaacacg cgtagccgcc cgcacaggga tgtgcgggcg gcggccgcga
     7141 gcggacctgg cccgcccctc ggcgcgccgg atcgaaggtg tgccgcctgt gcaggcctac
     7201 ggaacgaaca ccctgcccgc gagagtcgcg cgcagggcgc gctcccacaa cccggcgcgc
     7261 ggcgacggga cttcaggcgc ccgcggggct gtccggcaga taggtctcga acagcttgcc
     7321 gaggtcgccg gccgcgtgcg cgggcacctt gtagtaggtg cggatcacca cggagccgtc
     7381 gggctcgaag cggtaggtct cgatgctgta ggcgaagcgc tcctcgccac cttccttgaa
     7441 gtggttctcc agcagccacg cgacctcgtt gccgcagatg aacaggctgc ccgggtggac
     7501 gcgaaagcgc acggtcggct ggaacaggtc gtaggaatcc agcgcgcagt gctggaagcc
     7561 gtgcttcatc ggcgtgccca ccggatcgag catgctgaat tcgccgggcg ccaccgcgcg
     7621 ccagttggcg gcccagcccg cgcggtcgcc cgcgttccac agcttcacgt agttgtcggc
     7681 ccagttcttc agctgggcaa aagtcggcgt gctcattgct ggttcttctc ctggtgaatg
     7741 ggtggcggaa tttcagtgcc ccttgaacac cggcgggcgc ttctcgaaga aggcctgcac
     7801 accctcgcgt gtgtcctcgc tgatgaacgt gatgttctgc agcttggcct cgtaggaaat
     7861 cgtgtccgcg agatcgagct gcatggcctt gttcagcgcc tccttcgagt agcgcagcgc
     7921 cagcggcgcc ttcttcgcga tcgtgcgcgc ccacgccagc gtctcctcga gcaggcgctc
     7981 ggcgggcacc acgcggttcg ccagtccgag ctccaggcag cgcgcggcgc ccatcttctc
     8041 gccttccacg atcacctcga aggcgcgctt gcgcccgagc tggcgtacca ggtgccaggt
     8101 ggcgcctccg tcgggcacca gcgcgatcgc catgaacgcc tggtagatgt aggcgttgtc
     8161 ggccatcacc gtgaggtcgc aggccatcgc gaaggaactg ccgatgccgg ccgccgcgcc
     8221 gtttaccgcg ctgatgaaag gcttgggcgc ctcgctgatc gccatcagca ccggcttgta
     8281 ctcctcgtcg atctgctgct gcaccagcgg cgggacgccg tcggcgagat cggcgccggc
     8341 gctgaagccg cgcccctcgc ccgtgagcac gaccacgcgc accgcggcat cgtcgctggc
     8401 gcagcgcacc gcggccagca gctcgcggcg catctggccg ttgaaggcat tcatcaccgc
     8461 agggcggttc agcgtgatca gcgccacgcc ctcgtccacg gcatagcgca cggtctcgaa
     8521 ttcactcatc gcgggtctcc ggctggtcag ggaagtgtgc agtgcggaaa gtcgccgcgc
     8581 cggcggcgct tgtcaagacg aaccggccgg gcgacgcagc accagcgcgt tgcccagtac
     8641 gatcagcacc agcccggcca cggcgggcag cgtccactgg tagccctcga acagcgtcga
     8701 gagcagcagc gcgaccaccg gggtcatcat cgcgttgtag cccgcgcgct ccggaccgat
     8761 cagcgcgatc aggcgcagga aggcaccgaa agccacgatc gagccgaaca ccgacaggta
     8821 cagcaacgag gcggtatatg gcaggccggc ggggaaacgg aactcggccc cggtgacgag
     8881 cgccgcgagc gtgagcagcg ccgtgccgta gaacatgccc caggcgttca ccgtgatcac
     8941 cttcagcccg aagaccgcat tgcgcgtggc caggatgctg ccgagcgagg ccacgtaggt
     9001 cgaagccagc gccagcaaca gaccacgcag cgcggtgtca tcgatgctca gcctgccgaa
     9061 ctcgggccag aacagcagcg cgacaccgcc gatgccgatc agcccgccga aaagcaccac
     9121 cggcaccagg gggcgtttca ggaacagcgc cgcgttgaag aaattcatga acaccatggt
     9181 ggcgaatatc accgccacca cgccgctggt gaggtagacc tcggaccagt acaccagcca
     9241 gtagttgagc ccgaacaggc aggcgccctg caacgcgatg aagaagtgct cgcgcggcgc
     9301 gagcctgagc cgcgcgcgcg ccagcacgca ccaggcgaac agcagcatcg acgcgagacc
     9361 gaagcggtag atcaccgaga ccagcggatc gatgtccccg agctggaact tgatcgcgag
     9421 ccaggtcgaa ccccagatca gcacggtggt gaaatacagg atgtgcgccg acaggggcat
     9481 gttgcaggtc cggaacaggg gaacggcgga gcgccagcgc cgctatcctg caggtagaat
     9541 gggcgcctgt gcaaacccgc gtgcaaaggc gacgcatccg cgcgacggat gaccatcaac
     9601 ccgaggcgcg gcgaggggcg ggcccgtgcc gcgagcggac atgtggatgc cgaaatcgcc
     9661 gggagcgatt tcggcaacac gcgtagccgc ccgcacatgg atgtgcgggt ggcggccgcg
     9721 agcggcacgg gcccgcccct cgccgcgccg tcatacgaaa ggaccacgcc atgggaaaac
     9781 aacgctcgga ccaacccggg cccgcggccc ccgccacccc gaaccccttc gccgccctcg
     9841 cctcgttgcg cggcacgctg cccgagggaa cgggcggcga acccgccgcg gcaagcgccg
     9901 gcgacagccc gttcgccgcg aagctcgtca tcgcgcgcga acgcaagggt cgcggcggca
     9961 agaccgcgac gctggtacgc ggcgtcgcgc tgacgggcgc ggcgctggag agcttcgcac
    10021 gcgagatgcg ccaggccctc ggcaccggcg gcaacgtcga gggtgatgcg atcgtgctcg
    10081 cgggcgacca gtcggcgcgc gccgcgcaat ggctgcgcga acgcggggcg acgcgcatcg
    10141 tgatcggcaa ctgaatcgtg gcgcacgcca ccggctacgc gctcttcgac accgcgatcg
    10201 ggcgctgcgc gatcgcctgg ggcgcgcgcg acgatctgcg cgacctcgtg ctcgacctcg
    10261 acgacatcac ggaattccac cgccgcgtat tcgccaccgc ccgcgcgatc gcgccgggca
    10321 acaccatcac ctacggcgag ctcgcgcgcc ggctcggcac gccgggcgcc gcccgggcag
    10381 tgggacaggc actgggcgcc aacccgtttc ccatcgtcgt gccctgtcat cgcatcgtcg
    10441 ccgccgacgg cagcctcggc ggcttttcgg cgccgggcgg cgcgcgcacc aagcggcgca
    10501 tgctgcagat cgagggcgcg atcgcgcagg cagatattgg cttcgaatga gtaaccgcgg
    10561 gggcgcggcc tctcagttgc cgcgcctggc gccgatcccc tcacggaaca gccggtagaa
    10621 cgtggtgctg agcgcgatcg cgagcacgcc cagcgccagc agcatcacgc tgcgccgctc
    10681 gaggaaactc tgcccgagat agatcgtgcc cagcgcgtag ggcagttcgg tgagcgcgag
    10741 cgcggcgaga taacggtgga acgggtaacg cgccataccg aggacatagc cgggaatctc
    10801 cgacggcagc gcggcctgca gcagcagcac gtgcaggaag cgcgtgcgcg cgttgagctg
    10861 ctcgcgaaag ctcgcgatcc gccggctgcc cagcaggccc ggcgccagcg tgtcacccag
    10921 gaaccggccg accgcgaagg acgcgatgcc gccgagcaac cagccaaccc acagcagcgc
    10981 ggaagacgcc aacggccccc aggcgctgag gccgacgggc accagcagcg cgctggaaaa
    11041 gaaggccagc atcgccgaga gcatcgccag cagcacgaac agcagctggc cggcgagcgg
    11101 acgcgcgacg atctcggcct cgaggaaggc gatgagttgg gcactcgacg caaagagcgc
    11161 atcggaggca gcgatcagac ccgcgacgca gatcatcagc gcgaccgcga ggccgtgccc
    11221 acgcgcggcg ccgaggccgc gtctcaccgc tgcagcggcc agatgaacgg gatcgcggcg
    11281 atcgtgacca gcgccatcag caggttcatc ggcacgccga tgcgcaggaa gtcggagaaa
    11341 cggtagccgc cgggtccgta caccatcagg ttgcactggt agccgatcgg ggtggcgaag
    11401 ctcgccgacg ccgccatcat caccgccatc acgtagggca cgtcgtgcag gcccgcggcc
    11461 tcggtgatcg cgagcactac cggcagcatc agcatcgcgg cggcgttgtt ggtgatcagc
    11521 tcggtcagca ccatcacgct gaggtaggtg agcgcgagca gcagccaggc gtcgttgccg
    11581 gcggcggcca cgatgccgtt gccgagatat tccgccgcgc cggtcttctc gagcgcgttg
    11641 ccgagcgcga agctcgcggc gatggtcacc agcaccggca ggtcgaggga gcgccgcgcc
    11701 tcgttcacgc cgatgcagcg cgtcgccagc atcgcggcca cgccgagcag cgcggcattc
    11761 agcatgctca tgaggccggt ggtcgccagc gcgatgattg cgccgaggat cgcccacgag
    11821 atcaacgcgt gcgaatgatc gggcagttcg tgctcgagca cgttcatcag caggaagtcg
    11881 cgcgactgct tgaggcggcc caccaccgcg ggacgcgcct cgagcagcag caggtcaccg
    11941 ggttcgagga tgatcgagct cagccgcccg cggatgtgct cgccgttgcg cgccaccgcc
    12001 agcaccaccg cgccgtagcg gtcgcggaaa cgcccgtcgc ggatcgtgtg ccccacgacc
    12061 tcgcagtgct cggccaccac ggcctccacg aggcggcgct ccggcacgtt gcgttccagt
    12121 gcgggctggc tgcccaccgc ggccaccagg ccgttgatgc gcagcacatc gacgatggcc
    12181 tcggtctcgc ccacgaacac caggcggtcg ccgccgcgca gcatctcctc gggcgccacg
    12241 gcggtgatca cgctgccgtc acgctcgatc tcggcgaggt agatgcgttc cagatgacgc
    12301 agtcccgcct gctggatggt cttgcccagc agcgggccgt cgcgcgcgac cgcgacctcg
    12361 aaggtgaaac tgcgcgtgtc ggcgaaggtc tggtcggggc tgcggcgcac cttcagcaac
    12421 ttcggcgccg cgaataccac gaacagcgtg cccaccagcg ccgcgatcag gccgatcggg
    12481 gcgatgtcga acagcgcgaa gccgggcttg ccggtcagca cctggtactg gccgttgacc
    12541 accaggttgg tgctggtgcc gatcagcgtg atcgtgccgc cgagaatcga ggcgtaactc
    12601 agcggaatca tcagcgcgga gctcggcacc ttgatccgcc gcgcccactg gctcaccgcc
    12661 gggatcatgg ttgccaccac cggggtgttg ttcaggaagg ccgacagccc gaccaccggc
    12721 agcatcaggc gcaccagcgc gccgcgcggg ccggccgggc gtcccagcac gcgctccacc
    12781 accaggtcga caccgcccga ggcgcggatg gccgccgcga cgatgaacat cgcggccacg
    12841 gtcatcaggc cggtgttggc gaatcccgac agggcttcgt cgggctgcag gatcccggtg
    12901 atgctgagca gcaacaaggc ccccatcagc accaggtccg tggtgaggcg cggaatggtg
    12961 atcagcgtgg cgagtaccgc gacggtcagc gcgaccgtga accatccctg ccaatccatc
    13021 agcgtctctc ccggcgaata ctctctgcga ggcgcattct aaaaggaata agcaagccat
    13081 gaataatgat ttgcggatat taggatattc gtcgcgccca ctcatgcccc ggaacgaaaa
    13141 aaggccgcct gcaccgggtg caggcggcct ttggaagctt tcaggccagc gtcagaacgc
    13201 gaagcccagg cccaccatcg ggccctggaa cgagaagtcg gcctctgcgt cgccttcttc
    13261 gatctcgtag ttcacggtac ggtagccgaa gcggaattcc aggttgcagc agttattcgt
    13321 gtacaccagt tcgggagcca gttcccagac caggtcggaa tcaccggcgc caatcgatgc
    13381 cgtcgggctg aacgcccagt tcctgcccag cgcgacacgc ggacgcagca tcacgatgcc
    13441 atcgaccagg tcgtcgtcgg cattgaagct gccggggccg ttcttttcgt tgaattcgag
    13501 gtcgagcttg ccgtagcgca cgcccaccag caggtccacc caggaatcac cgccggcgcc
    13561 gaagcggtag ccggtcgctg ccgtggccag cagggtgtcg gcttccacct cgatctcgct
    13621 gcccgcaacg ttgatgtcgg tgtcgtcgtt ctcggtcttg atgtaatcga ccgcggcaaa
    13681 gttgctccag cggcccttgt tgtgctcgag catcagggag ccgttgaact ccaggttgtc
    13741 ggtgacgtca ccgatgtcct ggttgaagtc gatgtcggtg ctgccttgtt cgtcattgat
    13801 ctcgaggttg ccgtcgatgc ccacggccca gtagtagggg gtcaggcgga agcagcactt
    13861 gtcggcgtct tcggcagcgg cgggcatggc agcctgcagg gcaagagcgg cgacagcggc
    13921 tgcaaggaag ttttgtttca tcatggaaaa ttctcctgga caggtttttt caccgcaccc
    13981 gttggtgccg gctgagcaaa gtatggttct cacttcgcct ggtcgcacgg ttggtgagcg
    14041 tgaaaattac cccgcggcgc gcagccggac cgtgcggtcg gccccgcggc cggacgacct
    14101 gcctcaggag gcagcaatca gcatcttgcg acgaagaaac gcgatctgtg tcgaaagagt
    14161 caggttcttc gggcaattat cctcgcaccc gagcagcgtc atgcaaccga aaacgccgct
    14221 ctcgtcgccg atcagttcgt aaaactgcgc gtcgctgcgc gcatcgcgcg gatcgagccg
    14281 aaagcgtgcg atcttgttca atccggccgc accgacgaag tcctcgcgca tgcgcgccgt
    14341 ggcgcaggcc gccacgcagc agccgcattc gatgcagcgt tccagctcgt agatctgctc
    14401 ggccagcgcg ggctccatgc gctcctccag cgcgcccagg tccggctcct gctcgcgcgg
    14461 gtgcacccag gtctcgagcc gctcggccat gccctgcatc cactcaccgg tatcgaccga
    14521 aaggtcgccg atcagccgga acaccggcaa cggcgccagc gtgacgcggc tgccgagatc
    14581 gcgcgtcagg gtgcggcagg cgagcccggg ccgaccgtcg atcagcatgc cgcaggcgcc
    14641 gcagatgccg gcgcggcaga cgaagtcgaa ctgcagcgtc gggtcctggt tctcgcggat
    14701 ctcgttcagc gcgatgaaca gcgtcatgcc gtcggcctcc tcgatctcga agcgctccag
    14761 gcgcggcgtg ctcaccggat ccgaggggtt gtggcgcaac acgctgatct ccagcgtgcg
    14821 cggacaggcg gtgacggtct cgctcatgtg tcgtggtctc ccagcctcgc attgcgtcca
    14881 cgcagcggcg gcggcaactg gtgctcccag ggcatcagcg cctgctgctg cgcaaggcgg
    14941 ccggcgccgg ccgttgccgc gcgcacccgg tcgacctcgg cctggcgcgc cggcgtgtcg
    15001 gggtggtcga tgtagtcttt cccgccataa ccgcgccagc cgggcggcag ctccatgcgc
    15061 cgcacgtcga gcggctcgta ggcgagcacc ggcagcgtgg catccgggtc gggccaggtc
    15121 gccagcgtgc gcgcgagcca gtcgcggtcg ttgcgcacgg ggaaatcgct gcgaaagtgc
    15181 gcgccgcggc tctcggtgcg cgccagcgcg ccctgcgcca cgcacagcgc gagcttgagc
    15241 atgcgcggca gccggtaggc ggccaccagc tcggggttcg cgccgcgcgc ctggctgcgc
    15301 agcccgaggt cgcggctgcg cacgagcagc tgctgcaact gcgtgaccgc ctcggcgagc
    15361 cgctcgccgc tgcgaaagat gccgacgttg tccgtcatgg ccacctgcat ctcgcgcagc
    15421 agcgcgaagg ggctctcggg accgccgcgc gcggcatgct ccgcgagctg gcggctttcg
    15481 cgcgcgtgga attcgcgcac cagcgccatc ggcacatcgg ccgcgccgtc gggcgattcg
    15541 cagtggtccg cgatgaactc gcccacgatc atgcctgcca ccacggtctc cgccacggag
    15601 ttgccgccca ggcggttgaa gccgtgcatg tcccagcacg ccgcctcgcc ggccgcgaac
    15661 agccctttca gcgtgcggct ctcgccgcgg tgatcggtgc ggatgccgcc catcgagtag
    15721 tgctgcgccg ggcgcaccgg gatcagctgc tcggccggat cgataccgag gaaatagttg
    15781 cagatctcct tcacctcgcg cagccgcgtc tcgatgtggc ggcgcccgag caggcggatg
    15841 tccagccaca ggtgctcgcc aaagcgcgag cgcgcgccat gcccggccgc aatgtgctct
    15901 tccatgcgcc gcgacaccac gtcgcgcgag gccagctccg ccttgtcggg ctcgtagtcg
    15961 ggcatgaagc ggtgctcgcg gccatcgagc agcaagccgc cgtcgccgcg gcagccttcc
    16021 gtcaccagga tgccggcagg aaaaatcgcc gtggggtgga actgcaccgc ttccatgttg
    16081 ccgagcgcgg caacgccggt ttccagcgcg atcgccgccc ccatgccctc gttgatgatc
    16141 gcgttggtcg agacgcggta gatgcgcccg aagccgccgg tggcgatgca ggtcgcgcgc
    16201 gccgcgtagg ccacgagctc gcccgtggcg aggttgcgcg cgatcacccc atggcagcgc
    16261 gcgccgtcgt ggatcagcga gagcgcctcc atgcgttcgt gcaccggaat gctgttcgcc
    16321 accgcctggt cgctcatcgt gaacagcatc gagtgcccgg tgccgtcggc gacatagcag
    16381 gtgcgccact tgcgcgtgcc gccgaaatcg cgcgcggtga tgagtccgtg cgcctcgtcg
    16441 cgctcggtga gcgtgacgcg cttgccgttc atcaccacct cgtgctcgcc gcgctgcacg
    16501 cggttccacg gcacgcccca ggccgcgagc tcgcgcaccg ccttcggcgc ggtgttcacg
    16561 aacatgcgca ccacgtcctg gtcggcaccc cagtcgctgc cgcgcacggt gtcggcaaag
    16621 tgcacgtcct cgttgtcgcc ctcgcccatc gcggcgttgc cgaggctcgc ctgcatgccg
    16681 ccctgcgccg cggccgagtg cgagcgcttg gccggcacca ggctcagcac aatcgcctcg
    16741 aagccgcgcc ggcgcacgcc gatcgcggtg cgcagcccgg cgaggccgcc gcccacgacc
    16801 agcacgtcgg tctggatcgt gcgcatcagc cgccacctgc ttgcggcacc gcctcgcgca
    16861 gcgccacggg cacgtagcgc tcgccggcac ggtcgcggtg ctcgatgccg atcttcatgt
    16921 aggcggcgaa tgtcagcagg cccagcacga ggaaaaaacc ggtgatcgcc cacttgaggc
    16981 gcaccaggcg gcgacgctgc gcgcgcggat cggcgctgcg tggcagcagg ccccacttca
    17041 gcaccagccg gtacagcccg atgcccccgt gcagctcgac ggcgaacagc agcaccaggt
    17101 acagcggcca catgcgcccg ctgacgaagc ggtcggccga ttcgtaggga ccgatatcgc
    17161 ccgggtgcgt catcatcgtg tagaggtgca cgccggcgag gaacatcagc accaggccgg
    17221 tcgcgacctg catcagccac agccaggtgt cggcgtggcg caagtcgcgg gagtggcgga
    17281 acagggtgcg gtattcgcgg tagctggagg gcatgcgccg catggccgcg accgcgtgca
    17341 cgatcatgag caggaagatc gcccccgcga tcgccgtgac cagctgcggg tagggccggc
    17401 cgaagaagta gacgccctcg aagaagcgcg cgacgtggta catcgcgtcc ttgccgagca
    17461 ggatgctgga aaccagcagc atgtggcccc acatgaacag cgccagcgcg aggccgctgg
    17521 cgccctggac caggtccagc caggccggag cccggtcgat acgggaccgc gcaattgcgc
    17581 gagacgtcgg attgtccacc gccctgtcag gctccggtgc tgctgttcag cgcccgatta
    17641 tgccccaaag cgccgccgga tacgtcctgg cacgcgagcc tcgcctgcag gttacggcgc
    17701 ctcatgatgc gcatgcgcgt cgccggcccc gggttctggc gcagtctcgc gttacggtac
    17761 ggtcacaggt cgattgccag tcgctcgccg cgcgcgcgcg agacgcaaat cgcgattcgc
    17821 tgcgtctgcc ggtgctccag ggcggtgagc acggcgtcgc ggtgatcgac ctcgccctcg
    17881 accaccgtgc agatgcacga tccgcagatg ccctcgcggc aggacgaggc tggcttcatg
    17941 ccccgcgcct ccatcacctc gaggatgctg cggtcggccg gaacgttcag cgtgatgtcg
    18001 ctgcgccgaa ggtgcagcgt gaacggcgcg tcgtcgggct tcgaccctgc ggcatcgtgc
    18061 gcgaatcgct cgcaatgcag cgcctcggcc ggccagcccg cctgctgcgc cacgccgagc
    18121 gcggcgtcga tgaacggcgc ggggccgcag acatacagcg cacgcggctt cccggcatcc
    18181 cagcgcggga tcagccgacc caggtccacg cgcccgccgg cggcctgcgt gtcgaggtgc
    18241 gtgcgcacgc tcgcgctgaa cggccagcgc acgatgtcgt cggcaaaggg aaggaactgc
    18301 cggtcacgcg agcagacatg cagctcgaaa tccgcacccg cggcgtgcag cgaatacgcc
    18361 atcgccagca gtggcgtgat gccgatgccc ccggccagca gcagcgtgtt cgtgtcggcg
    18421 ggctgctgct gcagggcaaa caggttgcgc ggcagcgaga tgcgcagggt gtcgccgctg
    18481 cggatctcct ggtgcatgcg cagcgacccg ccacgtcccc tgtcctcgcg cttcacggcg
    18541 atacggtagc agccgcgatc tgccggatcg ccgcacagcg aataatggcg cacgatcccc
    18601 gtctcgatct cgacatcgat gtgggcgccg ggcgtccacg gcggcagttg tgctgcgtcc
    18661 ggcgcgacca gctcgtaggt acgcgcgtcc gccgatacct cgcgcacgct ggccacccgc
    18721 accgcatgca gcccgtccgg tcgcaggcgg cgcggcgaca tccgctcgcg cagcgagggc
    18781 agcagacctg ccgtcgcagc atcgcgccag ggcgtgaacc ccggcgaaag gaaacgcagg
    18841 aaatcaacca ggtagcgact gccctccgtg gcaaggtgca cccacggcat gaccgaagcc
    18901 gcggagcaac ccatgcgctg cgccatctgc ggcaccagcg cgcgccacat gcgcaccagc
    18961 gcgcgcacgc cgcagcgggt gccgcgggtg agtgccgcca ttcgccggct gtaggcgtcg
    19021 ggctgcgcgc cgtagatgta ctcgtacacg tcgtagacca cggccgtgtg ctcggtctcc
    19081 tccatgcagt gccacaacac caggcggtcc acctcggggt gcacacgccg gcgcaactcg
    19141 tctgtggtct cgtaccactc tgcaatgctg aagatcacgt gctcgagcgc ggccaccgcg
    19201 gcgagctggt cctgctgcgc gccggcggac atgtcgtgga tgaagcggcg gaactcgcgc
    19261 tcgatcacgg tcgtgtcgat gccgtggcgc tcgttcatca cggtgttgag cagcacgtgt
    19321 ccctgcgagt gcagcgcttc ctgggtgatg aaggcctgcg cctcgcgggc aagttccgga
    19381 tagcccgcga gtttcgccag gtggttgcgc accaccggga tgccgaagtc ctcgaaagcg
    19441 accacgaagt aggagatcgc caggatgaag gccgccagat tgggatcgtc ctccaccggg
    19501 cacgccggga ccccggagaa atcccagctc atccgccgcg gcgccaggtg aatgtgctgg
    19561 cgcattgtcg aggtgggacg gtaattggcg cacgcggcgg gggtcgcggt gttcatgatc
    19621 ggctctcctg tgacgacgtg ctccgtcccg agcttgcacg ttgcgacgtg gccagggtgg
    19681 atcggcatcc atcccggtca ctggcgccgg gattcgatgt accgggttgc gaacgcgatg
    19741 gcttcctccg gcttcgcggc gatcagcgcg gcctccttgc cggcggcgtc gatctcccag
    19801 ccgtccacga gcacgcggcc gatcatccag agtgccgcga cgcgggttgc cgaattgcaa
    19861 tggatgtaga caggctgatt cgcctcgtga ccgagcgcat ccaggaactc cccgatgacc
    19921 tcggtgtcgg gcgttttcgg acctgggttc ttggggtcga acggcaaatg gatgtatttc
    19981 aggccggccg cttcggcggc cacgcgaccc gcctcgatct cggcgcctgc ctcgctggca
    20041 aagcgcaagc cgatcacggt ggcgaagcct gcgccgcgta acgatgacat cgcggagggt
    20101 tgcgtcgcac caccgaaacc tgccgtggac ccggcgaaac cggaggagtg gtcgattcgc
    20161 gaaaagttca cgacacccgg catatcgact ttcacgggct gcgcgccggc ctgctctgcg
    20221 gcatgaccag gggcgaccgc gctggccgcc agaaccgcca cgagggcgat gcagggaaat
    20281 ttcatctgtt tcgctcgttt cagtaaatcc atgacaccgg actgcgctag cccatccgtc
    20341 gcgactccag ctggagctcc gaaggctcgg gcagcatctc ggtggttttc ttcttccacg
    20401 cctcctcgag cagttccttc gcagccattt cgaggaactc cggcgccgcc caggcatgca
    20461 cgtcgaaatc gttcttgatg tagccgttct tcagcatgaa gtccttgccg gtttcgatgc
    20521 agctgaggtt ctggtgcgac aggttcggca ccatgtcgac gtccttgatg ccccggtacg
    20581 tgtcctcggc atcacggtag aacgtctgcc ggtcgaggat cacggccgct gcctgcttga
    20641 actcgttggc ccagcgcccc accttgatca tgctcttcat gtaagtcacg acgagttccg
    20701 ggtgctccct ggccatgaca tcggtgcagg tgatgacggc cggcgtattc gcgacctgca
    20761 acttccagtc cggataccgc gacagatcct cgatcatccg gatctcgccc gtgtcttcct
    20821 gcaggtgctg gaataccttg ctctgcgtgt agatcgcatc gaccttgcca ctgagcagtg
    20881 cagactccag cgggcggaac gcgaggtcac gcttgtgatc gcgggtggcc cagaggtcgg
    20941 tcgggttgat catcggcgcc agcatcttgg ggtcgtcgta ccagtcgtcc gcgtagggga
    21001 attccacgat ctgcacgtct ttcatgctca tgtcgttcat catgagcatg ttctggatgc
    21061 ccatgtgttc ctggatgcgc caccagtcgt tcttgatcgt gttcaggctc ttcgaaaggc
    21121 cgattttctt tccccgcaga tcctccatcc gccagaggtt gtcgtcggcg cgaaccgcca
    21181 tgcagccgcc ttcgtacacc caggtcgcgc cgagcaggca tgtgcggcgg acatccgcgt
    21241 gcacgtgaat tggcggatag aggccaccga aacggatcag gttgtcgagg ttgtggatgt
    21301 agtgcgggta ccagtcgttc tcgcggcggc tgcgaaagta ggagtaatcg atcccgatgg
    21361 ccttgaaatc ggtcttgcac cagcccagtt cctgatcgat gttgttggcc gacaccatcg
    21421 gacagttggt gaaatacacc tccttccagt tcaacggcac cgttcgacct ttagtcgacg
    21481 tgccgccgga tagcgtttca gccgtcgcgc gcggggcgag tcctgcgccc agcgcaagcc
    21541 cgcctgccgc cagcacgccg ctcgaggcca cgaaattcct gcgcgatagc cccgaagaac
    21601 cctttttgat ctcgtccacg ccacgctccc cctcgattga atccgtatgc gctaccctaa
    21661 cgtgccccgc cgtcgcgccg gcacgcggga agcgacgatc aattggttta aactgacaaa
    21721 aagctctgcc gtcagccccc gcgccagggc ccgcgagccc cgcgaccgga gatcgacagg
    21781 tattgatcat gcgcacatcg cgaccttcag cgggcgagac tgttcacccg gtctacatcc
    21841 gtttcgtgtg cacgctgctg aggcagaacg gcaaggatcc cgaggcggtg ctcgccgcgg
    21901 tcggcctgga cagccatcgc gtgacggcaa gcagcgactg gctggagttc gacaccttcg
    21961 agcgatttct cgaagctgcc ggtaccgccc tcgggtgccc ctcgttcgga ctggacctgg
    22021 gatcgcgctt ccagctcgtc gcctacagcg cggtcggcca cgcggcggtc accagcgcca
    22081 cgctgcgcga ctcgctgcag gtcctcagcc gctttggcgc gttgcgattg cgcaacctgt
    22141 cggtgcgcta cctggaggag accgatggcg caacggtagt catgtacctg cgctgccact
    22201 cggaatcggc acgccggatc ctctacgagg tgctgctcgc actgctggtg cgcctcaccg
    22261 agtcggtcac cggcagcgac gccgactacc tcgacgtaca gatgccgttc gcctcgccac
    22321 cctgggtcgg gcgttacgcc atgttccgct tcgcgcggct cggcttcggc ggcgagcagc
    22381 tggtggtgcg ctatccgcgc gcagccctcg acaccgcttg cctcagcgcc gacgcgaaac
    22441 tgtgcgagcg tgcaacgcgc gaatgcgagg agcaactgca cctcgccgag acaacgccgc
    22501 tatccacgcg cgttcgcgag ctcgtgagcc ggagcctgga ggacacgcgc gtcgccgcct
    22561 gcccggaaat caccgacatc gcggaccatc tgcacgtctc ggcacgaacc ctgatgcgca
    22621 ggctcaagcg agaaggcagc agctaccagg ccatcgtgga cgagatccgc gccgagcagg
    22681 cgatctggct gctgaaatac tcgagcatgc cgattgcgga actcgccgca agactgggat
    22741 tcgaggacac ctcgagcttc aaccgcgcct tcaggcgctg gctcggcgtg gcgccgacat
    22801 cgctgcgcag gggcaggcga agcgggtgat ccggctcggg tgtgttgcgg tgccggttcg
    22861 ccggcggaac ttcaggtcgc agagacgcgg tattcgctgg gcgccttgcc cgtccagcgc
    22921 cggaacgcac ggttgaagtt gctcacgtcg gaaaagccga gcatgaatgc gatctcggtg
    22981 atggagagcc ttttctgccg cacatatccg agggccagct gctggcgcgt atcgtccagc
    23041 acctgcagga acgagacgcc ctggcgcgcg agcttcgcct gcaaggtgct ggggctcatg
    23101 tgcatgcgcg tcgcgatctc cgcgcggctg aatatgccgg cgttcagtcc gttcacgagc
    23161 tgcgtgcgca cacgattcgc gatgtcgtcg cggtcgagct tctcgagata ggtcatcgcg
    23221 atctggtcgt tgtgctgcgc cagttcgcgg ttggcgccga gcaggggttc gcgcatcgcg
    23281 gcgagctcga aatgaaaaca cagatgcggc tggccgaagc ggaccgggca gccgaaaaag
    23341 gcgcgaaatg gctcctcgcc gccggccggg accggacggc gcagatcgag cgcgcgggaa
    23401 cagaaatcgg cccgataggc catccgcatc aggcgcagga ccagggcggc ccagacgtcc
    23461 tgggtttcga cgcagatatc ggggttgcgc ggatccgcct cgagcctgaa cgtctgccct
    23521 tcgacgaaga cgcgcagctc ggcgctctgc gaaaccagcc cgtaatagcg cacgaagcgg
    23581 tggcagaaat cctcgagagt ctcgctcgcg agcagcgcgt agcccagcgc gtgcagcgtg
    23641 cccggcacga tgtgttcagc cacgcgcagg ccgaaatact cgtcgccggt cagcgcgacc
    23701 gcacgggcat agatcgcatt gaccgtcgag tcggtgatgc gcaccagcgg gtcgttgccg
    23761 tggatgcgca acacgtcgat ctcgcgcagc acctcgtcgg gatccgcgcc gcgcgcctgc
    23821 agcgcccggg tgatcgcctc gacgtaggcc gccacggtcg tcggttcgat caggctttgt
    23881 tttggcttgg gcatgagcgc gaagactagc cgctgccacg gggggccgtc aaagcccggg
    23941 gtgcgattgc gccaggacgg catccggtgg ccgcctgcgc agccgccagg atccgacccc
    24001 ggtcagagcg aggcgcgcgg caaccccagg tactcgagca gcagggggtt gaggcgtgcg
    24061 ggcgcatcca cctgcagcca gtggttggcg ccgtcgatcc gggcgtagcg ccagggtccg
    24121 cgcacgtagc gttcgctggc gatcatctgc ccctcgacga ggaagaggtc gccgctgctc
    24181 cagacgccga acaccggcac gcgcgccgag gggagctcgc cggcaaacaa ggcaccaagg
    24241 ttcgcgcggt agtagttcat gccggccgtg agccgccccg gacgcgacag ccgttcgcgc
    24301 cagcgcggga actcggccgc gtagcgcgtc aactggcgga acaggaacca gtcgccgcac
    24361 attgccagcc actccatgat gccgcgcagc tgcatcagca ggacgtagta gcccttgagc
    24421 ttctgcacca ggccgccgcg cgcgtaggcc gtcggatgcc cgaccgagag ggccacgaaa
    24481 cggtcgacgc gctgcggatg cgccagcacg aaccgccagc cgatcaccgc gccccagtcg
    24541 tgcgcgacga ggcgcaccgc gtcgatgccc agcgcatcga gcagcgcgcg caggtcctcg
    24601 accaggattt cgacgcggta atcagccacc cgcccgggaa tgcccgattc gccgcaaccg
    24661 cgcgtatcgg gcgcgatcac gcgatagccg gccgcgacga gcgcctggac ctgctcgcgc
    24721 cagaccgtgt ggtcgtcggg gaaaccgtgc accagcaaca ccggctcgcc ctcgccggcg
    24781 agcagcacgt tcatctcgca gccgttgatc ggcagacggc ggtattcagc gcccgtgatc
    24841 atctgtttcg gaacctccac tgactcgatc gacagccggc ccgggctacg ggttgcgcgc
    24901 gcgcgacacc tgctgtcgca ggccttgcag cgccgcgcgc agcttgcgcc gcagcagcac
    24961 ccggaacaag ccgcccagcc cggggatgcg tgagcggaaa cgcaggatcc actcgacccg
    25021 cgtaccatca ccctccggca ccagccctac ctcgccccag tagccgatga acggcgagcc
    25081 cgcgatgatg cggtaacgat agccgcgacc cggccaccag tcggtgatct gctcgcggat
    25141 cgtgccgagc gggctttcga gcaaacgctc ggcgacccgt ccgccgagct ccccgtcgcc
    25201 gcggcgtacc aggcggtact cgccgaaacc ggcccagtcg ctcatgccgc gatggtcacc
    25261 cagcaccgcg aagacttccg cggccggggc ctcgatacgc aacgacgcct cgacgcgctg
    25321 gttgtcgagc tgcgggcgct gcgcgcgcgg caacggcacg aaaccgaagg cgcgctgcgc
    25381 caggccgggg cgggcccaca tgatttccca ggagaagccg agcgggtcgc tcacgtacac
    25441 gcagcccgtg atgccgaggt tcagcggcgc acagttgtgc cgcgcgccgg ccccgagcgc
    25501 ccggcggtac agggccatga cgcccgcgct tccccgcggg tcgccgaagc agacgttgag
    25561 gatgcgctgg tcgcacaggc ggtagccggg cgggaatggt cggcccggcg gatcgcggta
    25621 ccgcaccagt tcgagcagca tcgccggatc gccgctggcg tcggtgaata cgcgccgttc
    25681 gcagcgcgca cccgcgagac cccacagcgc ctcgtgagcg tcctcgtgca gttgccgcgg
    25741 cacttcgcgc atgccgaggc cgcgggtgag gaaatccacc gaagcgtcca tgtccggcgt
    25801 cgacagggtg gcactgcgga tcgccaccgg gcaatcctgg cgaccacggg cgttttgtgc
    25861 cggcagcgga tcgtcttcca tgagctcgac gtacacgccg tccggattgc gcacgcaggc
    25921 gcgccgtctc cccggcacac cgaccggcgc ggccagcggc acgctgccga gccgcgcgag
    25981 gcgcgccagg gctgcatcga aatccgccac ccacacaccg cagcggctgt agcccaggtc
    26041 gctggtgcgg taatcggccg gcatcagctg ggaggccggc ttctcgtact ggaagagttc
    26101 cagctggcac cagtcgttgc gcccgagcag gcaccagcag gtgcacgcgg aaccgggcag
    26161 cttctggatg cggcccgata gcgggccgcg gaacaacgcc cgcgtgccgc ccgcaggaag
    26221 gaaacccagc ccctcgcgcc accaggcctc ggtgcggcgc aggtcgagca ccgcgaaggc
    26281 gatctggttc aggggcagga cggcctggct catggggcgt gcgctctggc gggcgcagac
    26341 gcgagcgggc gcgtcgcggc gctcgcggga tcgatgagga tcttggcatg ccgttccggg
    26401 tccttcagcg cgtcgaatgc attggccacg ccgtcgagcc cgaccacgcc cgtgatcagc
    26461 ggggcgcagt ccaccttgcc atcggcgatc aggtgcaggg tgtcgcggaa ctccagcggc
    26521 gagtggccga acacgaaacg caggtcgatc tccttgtgga tcgccacggc cggctcgatg
    26581 cggtcgacct gcatgcagac accggccacc acgacgcgcg acagcagtgg cgcgccgtcg
    26641 atgagctgct gcaggagccc cggcacgccg acgcactcga agatcaccgg tcgcgccggg
    26701 ttctgcccga gtgcctccgc gaggcgccac acgtgccacc agagcagcgg cagcttcgcg
    26761 agcttctcgc gcgtgccgag cgcgagttcc agcaggtcgc cgagcccgcc catgaaaccg
    26821 tattcctgcc agttcgacca gggcgatgtc gtggcgggat cgatcacgac atcggcgccg
    26881 caccgttgcg ccagcgcgcg acggcccggc gaaaaatcgc tggcaacgac ggtcttcacg
    26941 ccgcgcgcct tcagcaggca gatcaccgcg aggccgaccg gtccgcaacc gatcacgatg
    27001 gccacatcct tgcgcgcgat ctcgctgcgt cgcaccgcgt gccaggcgac ggccatcggc
    27061 tcggtgagcg ccgccatctc gctggacagg ccgctaggca ccggtaccag ggccgattcc
    27121 tgcagcaaca tgcgctcggc ataggcgccg ggggagcgca ccgagagtcc cagcatgtcg
    27181 atgccgttgc ccacgcgcag caagggctgc gccaccacgc gcgtgcccgg cctgatcctg
    27241 cgctcgcagc ccgggccatg gtcgagcacc tcgcagcaga actcgtggcc gaataccacc
    27301 gcgtcgccca cgccgggaaa ctgcgcgtcg aatgcgacgc gcgccatcag ctgcttcatg
    27361 tgtccgcagt gctcgcgcac gtgcaggtcc gagccgcaga tgccgcaatg cagcacctgc
    27421 accagcacct gcccgcggcc cggttcgggc tcgggcagat cggcgacccg caatccggtg
    27481 ttctggcaaa cgacggcttt cagattcatc tcctcgttat gggcatcacg cagcctgcgc
    27541 ttgcgctcgc ggcatgtccg gctgaagccg gacctacggc cgccaacgcc cgtgggtcgg
    27601 cattcatgcc gacattgtcc ggctgaagcc ggacctacgg cggcgtggcg cgcaggtgat
    27661 ggcgctggtg cgccggcagc aggctcgtcg tatccgtcgc gtcgggctgt atgcagacga
    27721 aggccggcag cttgtcctcg tgcgcgtggg ccggcaggtt cgagttcgag gcgcacacgc
    27781 gaccgtcctg cgtgaactcg aagtcgcgga accagatcac gcggttcggc atcgggtagg
    27841 cgacgaactt gcgctcggcc gggatgtaac gcatcacgcg gtcgctgttg ttggtggcga
    27901 tccacacatc accgttgcgg tccacgttga gcgcgtaggg cagctcgaac tcgttcggcg
    27961 ccagcagcgg cagcgcgatg ctgtcgaagt tcttgctgcg ggtgtcgaag cgcatcaggt
    28021 agccctcgtc gaaggaggga atccacaggc tgccatcggg gccgaagcgc agccgccgcg
    28081 gcccgccgtg cggcacgtcg tattcggtga ctgcgagcgt cttcggatcg atgtgaccga
    28141 tcttgttcga cagcagcttg ctgtaccaga tgccgccgtc gcgcggatcg atgtcgatgc
    28201 cgtacggcca gttgaacacg tcgcggccgc cgttcatcca cttgtggtgc gaaaggtaca
    28261 ggtggtagtt cttctcggga aaccacgaac agatcttcag caccacgccg atgaaggtat
    28321 cgctgatcca gcgcatcacg ccgttgtgcg gtgtggcgac ctcggtgaag cgctccgtct
    28381 tcgggtcgaa cttcatcacg gtgttggaca cgagcaccgt gaaccagaga ttgtcgtcct
    28441 tgtcggcgcg gatcgtgtgc gagtacagtc ccttgcgcca caggaagccc tgcgggatcg
    28501 ggtagagctt ccactccttc gtcgccgggt tgaacatcag caggttgctc gatagcgcgc
    28561 cggtgaagaa gatgcgcccg tcggagatct gcgcgccgct gtgcgggccg tggcggccgg
    28621 tgaagatgcc gatcggcagc tgcatgccgc ggaaatttcc acccaccacc tcgtcggtca
    28681 ccggcatctc gtatttttcg atctgtccgg agtggcgatc gaggatgtag acctcgtcgc
    28741 gtccttcgtc gatgcccatc aggcgatcgt cggcgagcac tatggtgtcg tgcaggaagg
    28801 acatcggcgt ggcggcatgc cactcctcga tgcgcgcttt cgcctgctcg gggctgaagt
    28861 cgtgctcctg tatcgcggcg acgggcttgc cggtgaagcc cctggccagc atcgcggcga
    28921 tgttcctctg ctcgcgccag gtcagcatcg cgagatagcc ttccatgcgg cgcacggtgt
    28981 ccgtccactc gctctcgctg cgtggccggc gagtcagcgc gttgccctgc tggtgacaga
    29041 agctgcactg ggaaatgaag gtctctttcg cttccccgtc ctggaatggc agcatggcgg
    29101 cgtgagccga tgccgtgagc gtgtcggaca gttcctccac cgaggcgatc ttctcgacgc
    29161 tgaaatccac cgcgagctgc tgcccggcac cgagctccac cgtctgcacc acgtcgcgga
    29221 aatacggcgt gcgcgcccgc acctgcagcg tgccggcgaa tccggtcggc aggctgtagc
    29281 ggcccgcggc atcgctgtag acggtttcct tgcggtcgcg cgccgcgttc cagacggtca
    29341 ccatggcccc ggcgaccgcc ttgccgtcgg aggtgcgtac gctgccgtcg agcgtaccgg
    29401 cggcggcgcc cgtgccgaga acgagcgcca tcaggccgaa ccaatggagg aatttcatga
    29461 gagaacctcg ctcagccgaa gtggtagaaa tagagatgca gcacggcgac ggcaagcgcg
    29521 cgggcggaat cagtcgcatt ggcgcgcggc ggcgcgtgcg cgcgccagcg cacggatcca
    29581 cgccgccgcg aaaaacacgg tcagcagcaa cagagccagc gccggcagcg catcgggcag
    29641 taccaggtcg cccttcatcg gcgtcagcgc gaccatcagc gcggcggcct tgtacggcag
    29701 gtactcgagg taggccggat cgccgatcag ctccaggaat gcgcggcggc tggggtaacg
    29761 caccaccagc acgcggctcc agttgtcgat cgccggatcg aggccgatca ggttggtgct
    29821 ggactgtgct ccagccggca ctccctgcat cgcgctgcca aacaaggggt atgcgccgag
    29881 cttgaacagc agcggcatca ccttctgttc gtacagcgca ttggcctgct ctggcgtgcc
    29941 cttgaagccc tcgactccgg gcatcgggcg caactccggg tagtagcgca tcaggttcag
    30001 catgtacacg ggcctgccgt cgtcggcttc gccccaggca cgcaggtgct cgaggatggc
    30061 gcgcttctct tcggccggcc acggcgcatg ctgctccagc atggtgagat ggcgatccac
    30121 ctcggcctgg ctcagcctgc tgccgggcgc gaaccaggcg gagcccgcca ggaacaaggc
    30181 aagcaacgcg acagccagca cgatctcgaa acgaaagtgc ttcatggtga ctccccgatc
    30241 ggctcagcag gtggcacggc tcgagacggc gtgagcccga tcagaacgcg gcgcgcacgg
    30301 tcagcccgta cgaacgcggc gcctggcgga agctctggta gctcttgtcg ttggcgaaca
    30361 ggttcatgct cgcatcgagg ttgaccacct cctcgtcggc cgcgttgtcg acccacgcca
    30421 ccacttcgta ggcgtcccag cgcgtgccga ggcgcaagtt gacccagttg taggcgtcct
    30481 gcaccaggcg cgggtcgatc tgggagctcg tgttgtactc gtccgaccac gaccagtcgg
    30541 cgcgcgcgta cacctccccc cagctcacgt cggcctcgta gagcagcccc acgtgcgtgg
    30601 tccactccgg cgcgttgacg ggatgctcgc cgctgaggtc gcaagtgccg ggaagcgagc
    30661 cgtccggcgt gcggcccgcg tagcacaacc cgctggtgta ctcctcgtag gtgaaatcgg
    30721 cgtaggagat gccgaagtcc gcactgactt tctcgccgag cagcagcttg ccctcggcct
    30781 cggcgccctt caattcggcc ttctcggcgt tggtgacatt gaacgtctgg ccgacgaagg
    30841 ccgcgtcctg gtagtcatcg tactcggtgt agaacgcgct gaccgccagc tgcatcgcgc
    30901 cctcgagcag cgtgctcttc acgccggcct cgtagtgcat gatgtcctcg tccttgaact
    30961 cgcgctggtc gatgggcgta cggccccagc cgacgttgaa gcctccagac ttgaagccgt
    31021 gcgagacggt cgcgaacagg ttcacgtcgt cgctgagcgt gaactgcggg gtgagcgacc
    31081 aggtcacctc gtcggtgtcg cggtccatct cgccgttgtt cgcgggcgcg gtactgatca
    31141 ggttggccga cagcaggctc agccccggca ccgtgagcga ttgcagcacc tcggcatcct
    31201 tctcctcggt ctgccagcgc gcgccgccgg tcaccgagaa gcggtccgta atgttccagg
    31261 tcgcctgacc gaagaggccg acgtaatcgg tgtcctggct ggcgtccagc agaccgttct
    31321 ggcccggcgt cgcgaagggt accgggatcg gcaagcgcaa cagcagctgc agcagccgcg
    31381 cggacgaagg gtgcgcgctg taggtgtctt cgaggaagat cggacggtcg ccgtcgtcgc
    31441 cgcgctcgaa ctcgttctgg taccagaaca cgccggtcag ccagtccacg gtctcaccgc
    31501 ccgcagaggc gaggcgcagt tcctgctgcc agctctcccc ctcctgggtg tcgtggaacc
    31561 gcagcagcgg cgaggcgagt tgcgccacgt cgtcgttcgt gcccttgaac tcgaaccagt
    31621 cccaggagct gatggaggtc acggtccagt cgttggccag cgcgtaatcg acgagcaggg
    31681 tcgcctcgcg cgcgtccacg tcgctggtca cggccttgcg cgagcagtgc tcgagatcgg
    31741 tcgcgtcgtt cgaggcgcag gcctcggcca ggccggcacc gcgcaaaaag gctcccgcct
    31801 gtgcggcgcc gctgcccggc tctatgtaga ggtcggaggt gaactggtcg tcgtcctcct
    31861 gcagcgcgcc gccgatcagg cgcacgctga gggtgtcgct cggctgccac tgcagctggc
    31921 cgcgcaccga gccgcgctcg aggtcgttgg catcctcgtc gctgaccgat agcgcgcttt
    31981 cgctgacgtg atcgttggtg gaataactca ggccaaggct cgcgctgagc gtatcggcga
    32041 gcgggccgct cacgccgccc acgaagcggt acatcccggc gtccgaggcg ccctcgaggt
    32101 tgccggccgt gaactcggca ttgcccgtga gctcctcgga aggcttgcgc gagtacacgg
    32161 ccacgacgcc ggccgtggtg ttcttgccgt agagggtgct ctgcgggccg cgcagcacct
    32221 cgatgcggtc gacgtcgaac atgtcgccgg ccgagaacac cgggcggttg cggaaggcgc
    32281 catcctggaa cacgcccacc gccggctcga aggtcgggat attgccgagg ttgccgacgc
    32341 gacgcacacg gtagctcatc gccagcggcg aggtgttcga ctgtacctgc aacgacggca
    32401 cctgccgcgc gaggtccttg atatcgctga tgccggcctt ctgcatcgcc tgtccgctca
    32461 ccgcggacac ggccatcggc acgtcctgca ggctctgctc gcgcttttgc gcggtgacga
    32521 cgatttcctc gagctgggcc tgtgccagcg cgccgagcgg cgcggcggcc agcgtgacgg
    32581 tgacgagcgc aagaagcttg cgggcagata aaatgcgggc ggtggtgcgc atgaagtgtg
    32641 tctcccgggg caatcacgtt gtgatgattg aaggctgttt gccatgagca tggtcgcgca
    32701 tcaccgcgcg gccggcctgt ccgcttcgcc tgactgcctg tcatcgcaac accgggttgt
    32761 ccgccggagc atgtcgcttg tccagcgagc cgagtcctgc acgccttgat ctgttcgata
    32821 tgctgtgaag cggggccggc ttaaactcgc cacgcaaatc ccgtatcgac ccgtaacgga
    32881 gggacacgac gatgaaccac ctgacgcgct ccgcgagctt gctgctggcc atcgcggccg
    32941 ccaccccgct gtatgcctcg acgctgatcc cggcgaaggg gcagacgccc gagcagatcc
    33001 aggccgacac cgccgcctgc cagtcgcagg ccacgcaggc ctcacagtcc gctgccccgg
    33061 caccggcgcc gcagggcggt cgattgaagg gggcagccgt cggtgccgcc gccggggccg
    33121 ctgcggcaca ggtacgaggc aaccagtacg aggcctatga ccggctcgac agcgacgtac
    33181 agcaggaata ccgccagaac caggcgcagg gggcagccgc ggccggcatg gtcgtggggg
    33241 gcgcaaaaca gcggcagcaa cggcggcaga cccagcagca ggccgctgcg cagagccagc
    33301 agcagggcag ccaggcattc gactcgtgca tgagcgcgcg cggctatacc gtccagccct
    33361 gagcgagagg caccggatcg ggcggcttcg ccaacgcgcg ctcattcgat cgctcgctga
    33421 tcgcgcctct cccgcatcac ccggcatgcg ggcccgctcc gtagccgcct cccgcgcggc
    33481 atccagcccc gccacgttca cgccgcccgg ggcggttcag cctgcttctg caaacgtacc
    33541 cggcttgaat cagcccttca aaccgagctt gtaaatctgg ttcgctgtct tccaggcgat
    33601 ctgctcacgc tcctcggcgg tcggccgggt ctcgatggcg gtgatgaggt tgtctgctat
    33661 cgaatgattc gacgagatgg cgcggaggtc gctgccgaac atgatccgtt gcgcgccgat
    33721 aacggagatc gcttcacgca ggtgctcggc cgaggtgtcg gtcatgtcga agtagacgtt
    33781 ggcgttgcgc atcgccacgg acaggcaggc atcgaaggac gcgcggatgc tccgcccagt
    33841 gcacgccttt ccagcccgtg tatccggtcg ggaacatgat gggcaggtcg ccccgcgcct
    33901 tgagcacttc catgatcggc gccatgtcgc gggtgatttt agccggatga atttcagtcg
    33961 tgatcggcac gatctcaccc acgccacgcg tggcgctcat ggcagggctc tgaagttcct
    34021 cggcgatctg acgcacaact ttgctgacct cgtcaccata gtccactccc agcgtccccc
    34081 tggcggccgc attcgaaatc gcttcgggcc ggtagatcgg gcagaatcgc ggaatcgtcc
    34141 aaggcatctt cgctaaaggc cttggcaaac ttttccggct gcctggtgcg aagcgcgccc
    34201 cagtcccggg cggtgtggat ggtcggcgcc agttctgcga cagtcggtcc ggagccgacg
    34261 tggcagtggc aatcgatcag gtacttcgtg ggcgcccagg atggcctggt gctgcgtccc
    34321 gccatgacag ggtcgtcatc agcgtacgct gcagaagaga ccagggcgcc cgccgcagcg
    34381 gccgaggcgg cggccgagag ctggagaaac tgccggcgcg aagcctcgct ccttgatccc
    34441 tcgggttttt gtacagcggg gccagcttgt tgctcgtcat ggcttgtgct catcgaactc
    34501 tcttctcgag tgtcggcgcg cgtttgaaac tacccagtgc gcgatcgcgg gcatggcccg
    34561 ctcctacagg gggcggtgcg cgatcgcggg catggcccgc tcctaccgta ggagcgggcc
    34621 atgcccgcga cctgttacgc ttcgatcaga acgagaagcc gaaaccgatg accggcccct
    34681 tgatcgtcag gtccatcgag tcgctgccct tctcgaaatc gtagttcagg ttgcggtagc
    34741 cgacgcggac atcgaaaccg cagtcgcagt cgtagacaaa ctgcggcgac agctcccagg
    34801 tgaggtcgga gtcgccggcg ccgatcgaca tcgtcgggct gaaggtccag ttgcgcgaca
    34861 gcgcgaggcg cgggcgcagc gcgaggatgc cgtcgtagac gttgttgtcc gcatcgcgct
    34921 gccccacccc ctcgatccag agttcggtgt ccatccgggc gtagcgtata ccgacctgca
    34981 cgtcgatcgt ggagcgttcg cccatgtgca cgcggtagcc cgtggccacc gtggccaggg
    35041 tgctctccat ctcgaggtca gcatcgatgc ccgggtagcg ggtgtcgaca tcgccgctgt
    35101 ccaggctcag gtaatccacc atcgcgtagc tgacccagtc gcccacgttg gcctcgaaca
    35161 gcgccgaggc ggccatgtcg aggttctcga ccacgtcctg gaagtccatc gagacagtgg
    35221 tgtcctcgcc gtgcgcctgg atgtcgccgt cgatgccgac ggcccacagg tagggcgtga
    35281 ccgtgaagcc gctgtccgct gccagggcag cggcagcggc agcggcgcct tgcagggcaa
    35341 ttgcggcgac ggccgcgccg agaatcttgc gcatgtgtct tgtgtccatt gtttgctgct
    35401 aaaaggagta acaggataac ccggcgccgc tacagcgcac gccaggctca gggcttgggc
    35461 tcggccagca tgtccgccgg tggcacgaac gcccgcttga gcttcagcgg cggaaagccc
    35521 gggtcctcgg gcggcgggtg ctcgggatcc gtcggctcga acggcgagcg gctcggcagc
    35581 accacgttgt tcgccttgac atagtgatcc cacagcgcag tgagctcccg caccttctcg
    35641 ggctgcgcgg cggcaagatc gttgcgctcg gcgggatcct cggcgaggtt gaacagctcc
    35701 caggcctcct tgccgaaggg ccggtactgc cagcgcagtt tccagtcacc ctgcagcacg
    35761 gcgcggttgc cgaacagttc ccacgccatg tagtcctgcg cgctcctcgg cgaatccgcg
    35821 ctgccggcca gcaccgcgct ccaggacttg ccgatcagcg gcggcagggc cttgccctcg
    35881 cgcgttgcgg gatacggcgc gccggcgatc ccgagcagcg tgggcatgat gtcggcaacg
    35941 tgcatgcggc cgttgttgat gctgcccggc gcgcgcttca ccacgggccc ggagacgatc
    36001 agcgcgttgc ggatgccgcc ttcgcccagc cagcccttga actggctgaa cggggtcatc
    36061 gacacctggg cccacatcgg gccgtaagcc acgtacgatc cgggctcgcc ccagtcggcg
    36121 ggatcgcgcc tcggccagtg ctcctgcgcg aacttgaagt ccggcgtgcc cggcgtgccc
    36181 gtgatcatcc ggaacaggtc gcttccctcg gcaccgttgt cgccgaacac gatgaatacc
    36241 gtgttctcgt actcgccgat ttccttcagg tgtgcgacga gccggccgat gtgatgatcg
    36301 aagttctcga ccaggccggc gtagagttcc atcttcctcg ccagcagcgc gcggccttcc
    36361 gggtcgagca gctcgggatc cggcgaggtc cacatgggtt cggcgagctg cgtgcccgca
    36421 ggcacgatgc cgagctcgac ctgccgtttc aggcgcgcct ggcgcacggc gtcccagccc
    36481 ttgtcgtaag cgcccgcgtg gcgattgcgc cagtcacgcg gcaggtgata gggatcgtgc
    36541 ggcgcctggt gcgcaacgta ggcgaagaaa ggcttgccgt cgccgcggtt ggcgtcgatg
    36601 aagccgatca gcttgtcggt gtaggtcttc gtggcgtagt agtcgtccgg cagccgcgtg
    36661 aggtagcgtc cgtcctcggt gaacacggac tgcggcgatg ccgcggtgaa gttggtcatg
    36721 tcccagtagc tgcccgcgcc gtcgagcagc gagaaatcgc gctcgaagcc gcgcgcggcc
    36781 ggaatcaggt cgggcgcctt gcccagatgc cacttgccga ccatgtaggt gtggtagccg
    36841 gcatcgcgca gcaattccgg cagggtggcc acgccgtggc tcaggtgacc ttcgtagccg
    36901 gcaacgccgc gctggttcgg cgccgtccac tcgtccatgt tgccgaggcc gttgcggtgc
    36961 gtgtcgacgc ccgacagcag catcgagcgc gtgggggaac aactcgcgtg cgtgtagaag
    37021 ttcgtgaagc gcacgcccga gttggcgagg gagtcgatgc ccggcgtgcg gatctcgctg
    37081 ccgaaggcgc cggtgtccgc gtaacccagg tcgtcgccca ggatcacaac gatgttcggc
    37141 cgcttggccg gcggcgccgc gagaaccgcg gcggatgcaa gggacaatgc gcaccacccc
    37201 gccactgcgg cgatcaggag caggaggcgc gggatcgccc cgaggcgttt cagggtctgc
    37261 tgcgagctgg ccatagggtc tccgtacgag cttgcgtcgt tcaggcgact gcggttacga
    37321 gccagcccgc gccatccatt gcatcgataa ccgcccgggt cgtcacgccg aaccacgagg
    37381 atgttagcag ccatcttcgg gcacgggatg atttgcaggc ataatcagct gataatcaac
    37441 cagcattttg gcccgagcta tcggaattcc gcagaattca atgagcagcg cttcgccaga
    37501 gaacaaggcc cctgccgcgg tcacgatcga cgagatctgc gacccgacga ctgccagcgc
    37561 cgggatcgag ctgctcgatc aggacgcggt gcaactgcag tcgatgccgt tgcgtgtacg
    37621 gcgggtgatc gtgcgcctcg atgaggcggc ggtggtgctc cactcgagca atgtgcgcgt
    37681 gcgcacgcat accagcatcc gcgagggctt tctggcctac gtgaccttcg gcccgcaggc
    37741 cagcgggaca gtgaacggcc tgcccatccg tcccggcctg atgctggccg ccgagccggc
    37801 gaccgaggtc ggcttcgttg ccgcccccgg ctgggagagc atcaccttcc tgctcccccc
    37861 ccacatcatg agcacgcagc tgagcgcccg ccatcgcgag gccgagtttc gtctgccgcg
    37921 cggcgtcgag gtgctgcagg cgaacccgga gatggtgcgc gcgctgcacg actggggcag
    37981 gcgactgatc gacacggcca ccaccacgcc cgagctgttc gatcaacgcc ggcgggaaat
    38041 gggcgcggtg caggtcgaat tgatcgagct gctgctggcg acgatcgggg ccgccgacga
    38101 catcgcatcg agccgcagcg atcgcacgcg ccaggcccag agccggatcg tgcgcagcgc
    38161 cgagaagtac gccctgtcgc atatcgccga ccacgtctac gtcagcgacc tgtgccgcgc
    38221 cgccgcggtc agcgagcgca ccctcgaagg cgcgttcaag gagatcatgg gcctggggcc
    38281 ggttgcttac ctgatccgcc tgcgcctgca ccgggtgcgg caggcgctgc tcggcgcgac
    38341 gcccggatcg accacggtgt cggccaccgc gctcgactgg ggcttctggc acttcggcga
    38401 attttcgagc gcctacaagg actgcttcgg cgagctgcct tcggatacct tgcgacgcgt
    38461 gccggcggca ggccgggaag acacgaagcc atcttccacg cgcaagcgca ggccttcccg
    38521 accgcgcgga taaatctgcg gttttccgat aggcctcgtt cctcccctgc gtcaatctgt
    38581 gccccatcga tccgcaatag cgatcttcaa ggggagcacc atgataattt cgaacccgta
    38641 ccagacccgg tcccgccacg acgacgcacg cggcaagcgg cgcatcgcga tgcacccgcc
    38701 cgggcgcacg attcttgcca tcgccctcgc catcgcggcc ggatccgccg gcgcgcaaaa
    38761 tccgggcggg cagctcgagg agatcgtggt cacggcgcag aagcgcgagc agaacctcca
    38821 ggacgtgccg atggccgttt ccgcgttcag cggcagcgcg ctgcagaacg ccggggttgc
    38881 cgacatcgag gatctcgccc gccaggtgcc cgcgctgcag gtacagacga atacctcggc
    38941 ggcgaccacg aactaccgca tgcgccgggt cggcaacctc ggcagcatcc cgaccttcga
    39001 gccggcggtc ggcgtgttca tcgacggcgc attccgcaac cgcccgctct tctccgcggg
    39061 cgacatgttc gacatcgagc gcatcgagat cctgcgcggc ccgcagagca cgctgtacgg
    39121 caagaacacc acggccggcg tggtggccat gtacaccagg gcgccgacgg aggaattcag
    39181 cggcagcggc gaattcacgg ccggccagat cgaggccgcc gcggatgcgg cgagttaccg
    39241 cttcgtgggc ggcctcagcg gtccgctgac ggacacgctc ggcgccagcc tcggggtgag
    39301 ctacaccagc aacgaccatc tcgacgagag cgcgctctcc gtcagcaacg acgacgccaa
    39361 tgacctcgaa cgcatggcgc tgcgcggcca gttgcagtgg cagccgagcg acgcgctgag
    39421 cgtgcgcctg ctggcgggag ccacggacga agacgacgag accatgacgc ccgacaccta
    39481 tatcgcgccg ggcggccccg cggcgaccgc gggcggcgtg ctgcgccagt tcggcctggc
    39541 ggagacgtgc agctcgaacg atccgctcga tcgcgagctc tgcgcgcgcg tacccaatac
    39601 caccgacctc cagtcgcgcg aagccacgct gctggtcgac tacacgctcg gcaacgactg
    39661 gaccgtgacc tcggtcagct cctgggactg gttcaaattc aagggcacat cggacgatat
    39721 cgtgcagctg gcctcgccgt tgctgaagtt ccacgacacc caggagggcg agagctggca
    39781 gcaggagctg cgcctggcct ccgcgggcgg cgaaacggtg gactggctga cgggcgtgtt
    39841 ctggtaccag aacgagttcg agcgtggcga cggcggcgat cgcccgatct acctcgagga
    39901 cacctacagc gcgcaccccg tgccctcgtt gttgctgcag cgcctgttcg gtacgccgtt
    39961 tcccgtgccc tttgccgcgc cgggccagaa cggcctgctg gatgcgcagc aggacacgga
    40021 ctacctcggc gtgttcggcc aggccacctg gaacctcacg gagcagtttg cgatcaccgg
    40081 cggcctgcgc tggcagaccg aagagaagga cgcctcgctc gagcagtcgg ccacggctcc
    40141 gggcgccagc ctgatcacgc tcagcctgct gcccgcggca atcggcggtg acatggaccg
    40201 tgacaccgac gagctgacct ggtccatcac gccgcaatac gcgctgaccg acgacgtgaa
    40261 cctgttcgcc accgcggcgc acggcttcaa gtccggcggc ttcaacatcg gctggggcag
    40321 gacgccgctg gtggatgcca gcggaaacaa cccgcgcgag ttcgacgacg agagcgtcat
    40381 gcactacgag gccggcgtga agagcacgct gctcgacggc gcgatgcaac tcgcgttcag
    40441 cgccttctac accgagtacg aggactacca ggacgcggcg ttcatcggcc agcagttcag
    40501 cgtgggaaat gccgagacgg cggagctcaa gggagccgag gccgagggca aggtactgct
    40561 cggtgaacac ctcaccgccg atttcgcgat ttcgtacgcg gacttcacct acgacgagta
    40621 cagcagcggg ctgtgctacc cggggcgcgt cccggacaac gccgccacgg gcgcctgcga
    40681 tctcagcggc gagcatccgg tcaacgcccc ggagtggacc acgcacctcg gcctgatcta
    40741 cgaaaccgac gtcagctggg gcgatgtcta cgcccgcgcc gactgggcct ggtccgacga
    40801 gtacaacacc agcttctcgg ccgatccgcg cctggtgcag gacagctacg actggctgaa
    40861 cttccggctc ggcacgcacg tcggagagaa ctacgagatc gtcgcgtggg tcgacaacgc
    40921 cctcgacgag gacgtggtca acttcgacgc ccagctggcc ctgttcggcg ccgacaagag
    40981 ctaccagacc ttccgccagg cctcgcgctc ctacggcctg acgctgcgcg cggagttctg
    41041 agagaccggc atggccatca tcgatttctt cgaccgtggc tggggcctga accccgacgg
    41101 cgccgcctac atccaggacg agcgccgcta cagcttccgc gaagtcggcg aactgtcctg
    41161 ccgcatcgcc aaccggctgc tcgccctggg cctgcccaag ggcaccaagg gcgccgtctg
    41221 gtcggtcaac gacgtcacgg cctggacctg cacgctgggc ctgtggcgcg cgaacatgag
    41281 ctggatcccg gtcaacgagc gcaacgcggc gcaggagaac cactacatcc tcgaggcctt
    41341 cgattgcgag gtgctgttct tccaggcgca tttcgccgac gtcatcgccg ggctgcgtcc
    41401 gcggctgccc gggatcaggc actggatctg catcgacgcc gacgcagcgg atgcgccctc
    41461 gctggagcgc tggtgcgccg gccagcccgc gacccgcccc gaagtcgccg tcgacctgga
    41521 cgacgtgatc gcgctctcgc ccaccggtgg caccaccggg atgcccaagg gcgtgatgaa
    41581 cacccaccgc agcgtgcaga ccttctttgc ccatttcatg ctggcctgta cctatcgcga
    41641 cggagcgacg ccggtgaacc tggctgccgc gccgatgacg cacaccgccg gcctgctgtc
    41701 cgtgccctgc acggcacagg gcggcaccgt ggtgatcgtg agcaagcccg atccggccct
    41761 gctgctgcaa gccgtggtcc ggcaccgggt gacggaattc ttcctgccgc cgacggtgat
    41821 ctaccggctg ctggaggctc ccggcatcga aaccgtggac ttctcctcgg tcagatacct
    41881 gctgtacggc gccgcgccga tgtcggtgga aaagctgaag aaggccatca ggctgtgggg
    41941 gccgatcatg gccggtggtt acggccagac cgaggccatg gccagcatct gcaacctgcg
    42001 cccggacgag cacatggtcg acggcaatct cgccgacgac acgcgcctgt cctcggtcgg
    42061 gcggcccaac gcgctgatcc gcgtagagat cctggacgac gccaaccgga tcctgccgcg
    42121 cggggagacc ggggaaatct gcgtgcgcgg cgacctggtg atgaagggct actacaaggc
    42181 cccggagaag accgccgaga ccgtcgtcga cggctggctg cacaccggcg acatcg
//
LOCUS       DAORUQ010000005        41108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_574_length_41108_cov_6.334957, whole genome shotgun sequence.
ACCESSION   DAORUQ010000005 DAORUQ010000000
VERSION     DAORUQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 41108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41108
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_574_length_41108_cov_6.334957"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLW13_00960"
     CDS             complement(<1..451)
                     /locus_tag="PLW13_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQY68967.1"
                     /translation="MDIQLARHGFDDRIRWDPALIRKYDVPGPRYTSYPTALQFTDGF
                     GAREYEQYLLERPPVIGPLSLYVHIPYCRNICYYCACNKIVTRDAAVAERYIVAVEKE
                     IALLSRHFDRRRRVTQLHWGGGTPTFLSPAELTRLMHGLAKHFSLIVA"
     gene            687..1367
                     /locus_tag="PLW13_00965"
     CDS             687..1367
                     /locus_tag="PLW13_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HQY68968.1"
                     /translation="MKKVTKISLLAAAMLVSGAASAYEAGDWIVRGGLVNVDPNDDSS
                     NLSVEGSRLGGTQVDVDADTQLLLNVTYMASSHVGVELLAATPFEHTVSTGGLSRYGL
                     NDVDLGEIKHLPPTLSALWYPMPASSKFQPFIGLGVNYTVFFDEDVSREAQNALGADN
                     LSLDDSWGLAGRVGMDYMLNDCWSLHAGAYYIDISTDAEVDTALGAAKVDVDVDPWVY
                     TLGLGYRF"
     gene            complement(1377..2078)
                     /locus_tag="PLW13_00970"
     CDS             complement(1377..2078)
                     /locus_tag="PLW13_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY68969.1"
                     /translation="MTPDPGILSALLLGLLGSTHCIGMCGGISGALAFALGPDLRPAQ
                     RYMLLTGYSLGRIMSYAVMGALFAGAAGGLAGDGGMRVLRIVAGVLLVLMGCYLAGWL
                     NALALLERAGHGAWRHVQGMAARLMPVDRLHKALLAGMAWGWLPCGLVYSTLTWAGTS
                     GSAARGALLMLAFGLGTLPAVVASGALANELRALLQRRGLRVIAGLLVIAFGVWTLAI
                     ALPHGQDPHAQHHHH"
     gene            complement(2075..2281)
                     /gene="ccoS"
                     /locus_tag="PLW13_00975"
     CDS             complement(2075..2281)
                     /gene="ccoS"
                     /locus_tag="PLW13_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQY68970.1"
                     /translation="MDSIYVLIPVTTILLGLAVAAYLWSVNSGQFDDLDKASQSILFD
                     DTDTVAPNDEAPATPPPAADGERP"
     gene            complement(2274..4703)
                     /locus_tag="PLW13_00980"
     CDS             complement(2274..4703)
                     /locus_tag="PLW13_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQY68971.1"
                     /translation="MTVACFHCGLPVPPGSRFLADIDGESRAMCCAGCAAVASLIAGS
                     GLAGYYRHRDTAAAAPADAGAGAAEYAVFDAEDAQGDFVLHEDDGYCRAQLAIDGINC
                     AACTWLIEHHLQRQPGVAAISVNLAEQRASVRWRPGELPLSQLMGRIRELGYRPYPFL
                     ASGRAETQRAENRSALKRMGLAGIVQMQIGMFAIALYAGDFAGMEADYRAYLRWASLV
                     LCVPVVAYSSAPFFSGALRGLRHGAPGMDLPIALAIALAFGASVWFTVAGGGAVYYDS
                     VAMFTFFVLLGRYLEMRARHRAGLAGGTMLSLVPAAATRVLEGGERHELVPVGRLRPG
                     DLVLIRPGEAIPADGAVLGGESSVDESSISGESLPVAKAAGDRVSAGTLNTDGSLTVQ
                     VQATGMNSRLGAILALVDRAQQEKPAIVQLADRSSRWFVLAVILVSVLTGLWWLQHEP
                     ARVVEIVLSVLVVSCPCALSLATPAAMTAATAALRARGFLVTRGHALDGLAAADTVVF
                     DKTGTLTTGEVKLAEVRTTGERDAASCLRIAASLEALSEHPIAAAFAEQARDPVEAFR
                     AHPGGGLEGVIDGRCYRLGRPGFALAGDAPAPPEVGKWILLACDGQALAWFRLTDTLR
                     ADATGAIAALRRAGLGVALLSGDAAGEVQRVATQLGVDEWRAGCVPEQKLAAVRELQA
                     SGRRVVMVGDGINDVAVLAGAAVSIAMANAADVTRANADCMLLAGDLRRIPDAILMAR
                     RARHVIRQNIAWAIVYNFAAVPFAAAGLVPPWLAAIGMSASSLVVIANALRLRNDAPA
                     STSLRPAGANG"
     gene            complement(4708..5208)
                     /locus_tag="PLW13_00985"
     CDS             complement(4708..5208)
                     /locus_tag="PLW13_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQY68972.1"
                     /translation="MNESAPWYRQGWPWFLILLPLTVVIACFVTLYLALSTDEALVRD
                     DYYKEGLAINQRLEAEANARARGIEAQLRYDAATLAIELQIAAAPAPELTLTIVHPTD
                     DERDQAVVLQAAGNGLYRGKVGAALDGKRYILLEHEDGPQGAWRLRGTFVPGSESVTS
                     LRLDAD"
     gene            complement(5205..6626)
                     /gene="ccoG"
                     /locus_tag="PLW13_00990"
     CDS             complement(5205..6626)
                     /gene="ccoG"
                     /locus_tag="PLW13_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQY68973.1"
                     /translation="MTDQRIPVIEVTPPSEVGEIDLYQKRERIYTRKVEGFFQRLRLY
                     TGWPLLILYFGAPWFNWDGRQAILFDLPARKFHIFAVTFWPQDFPLLAWLLMIAAFGL
                     FTVTVFAGRVWCGYTCPQTVWTSIFMWAEQVSEGTRNQRIKLDQGPWTVNKVWRKSLK
                     HGMWFGFAAFTGITFVGYFTPIRALVIDLPLGQAGMWANLWTLFFTLATYINAGWLRE
                     QVCMYMCPYARFQSVMFDHETLVVSYDRKRGEPRGSRKREADPKALALGDCIDCELCV
                     QVCPTGIDIRNGLQYECITCALCIDACDSVMDKMNYPRGLIRYTTENALDGKPSRIMR
                     PRLAGYSLAVLVLTSLFLSVLFNRIPVGIDAIRERGQLFREVDGGLVENVYTLKIRNM
                     GEKEMSYRIAASGIEGASIVGPTRVTIQSGDVLALPIALQVPRESLQGNSNAEVRFTV
                     KAVGQGGIQDTAESRFLGPGPAR"
     gene            complement(6721..7599)
                     /gene="ccoP"
                     /locus_tag="PLW13_00995"
     CDS             complement(6721..7599)
                     /gene="ccoP"
                     /locus_tag="PLW13_00995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HQY68974.1"
                     /translation="MTSFWSAYIIVLTAITIVGTYWLLFGNRSRPSDSEAKTGHVYDG
                     IEEYDNPLPAWWLYMFVITGVFGVGYLVAFPGLGNFQGLLGWSQEGQWKREVQRAEAR
                     FEPVFAAYRDKSIEQIAADPQAVRMGQRLFANNCSVCHGSDARGSVGFPNLTDADWLY
                     GGSPEAIHATLVNGRQAAMPPWEAALGDQGVTNVAHYVRTLSGAEPASAESAAGEAQF
                     KMFCVACHGADGKGNQMLGAPNLADNIWLYGGDLETIKHALRKGRNGQMPAFGEQLSE
                     DKIRLLTAYVYSLSSQ"
     gene            complement(7592..7765)
                     /locus_tag="PLW13_01000"
     CDS             complement(7592..7765)
                     /locus_tag="PLW13_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HQY68975.1"
                     /translation="MDRGDLLGVATILAMIAFLGVCVWAWSGKRKQRFDEAANLPFAD
                     DDLDGTKKGRSDD"
     gene            complement(7775..8383)
                     /gene="ccoO"
                     /locus_tag="PLW13_01005"
     CDS             complement(7775..8383)
                     /gene="ccoO"
                     /locus_tag="PLW13_01005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQY68976.1"
                     /translation="MQHEKIEKNIGLMIVLIIVAISFGGLVEIVPLFFQKQTTEPVAG
                     LKPLTGLQLEGRDIYIREGCNVCHSQMIRPLRAETERYGHFSVAGEFVYDHPFLWGSK
                     RTGPDLARVGGRYSDDWHRAHLYDPRSVVPESVMPSYPWLFENTLDGKDTPAKMRALR
                     LVGVPYTDEDIAGAEKAVAGNKEIDAVVAYLQQLGTLLKSTR"
     gene            complement(8396..9829)
                     /gene="ccoN"
                     /locus_tag="PLW13_01010"
     CDS             complement(8396..9829)
                     /gene="ccoN"
                     /locus_tag="PLW13_01010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HQY68977.1"
                     /translation="MTHPDATEHPVYNYKVVRQFAIMTVVWGIVGMLVGVLIAAQLAY
                     PALNFDLPWLSFGRLRPLHTNAVIFAFGGSALFASSLYVVQRTCQARLISDGLASFVF
                     WGWQLVIVLAAVTLPLGYTSSKEYAELEWPIDILIAVVWVAYAILYFGTIMKRKMSHI
                     YVANWFFGAYIITIAVLHIVNSAAVPVSLTKSYSAYSGTIDAMVQWWYGHNAVGFFLT
                     AGFLGIMYYFVPKQAERPVYSYRLSIVHFWALIAIYMWAGPHHLHYSALPDWAQSLGM
                     VMSLILLAPSWGGMINGMMTLSGAWHKLRSDPTLRFLVVSLSFYGMSTFEGPMMSIKT
                     INALSHYTDWTIGHVHSGALGWVAMVSIGAMYHLIPVLFGRKDMHSIKAINAHFWLAT
                     IGTVLYIASMWVNGIMQGLMWRAYNNDGTLTYSFAESVLASHPGYIVRLIGGAMFFLG
                     MLIMAWNTWKTVSPGTAPVTNAQAQPA"
     gene            10221..11183
                     /locus_tag="PLW13_01015"
     CDS             10221..11183
                     /locus_tag="PLW13_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQY68978.1"
                     /translation="MRSVILDAASLGEDVDLGPVLSLPGPWVCHEATPAAEVAPRIRD
                     AEVVLSNKVPLAADSLAGARRLRLVVVMATGTNNVDLAAATRHGIAVCNARGYATASV
                     AQHTMLLLLALARSLLPAERSARDGTWSRSPFFCVPGHPTLELGGRRIGIVGYGELGR
                     EVARLARAFGMQVALAALPGRRYADAQDHARMPLAQMLPWADVISLHCPLTPMTRGLI
                     GAPELARMRATALLINTARGGIVDEHALLDALRAGRLGGAALDTLECEPPRADSPLLN
                     AGLPNLIITPHVAWASREARQRLVGQVGEILREYRAGRFMNRVN"
     gene            complement(11458..14220)
                     /locus_tag="PLW13_01020"
     CDS             complement(11458..14220)
                     /locus_tag="PLW13_01020"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQY68979.1"
                     /translation="MDKTYSPDEIEGRWYAQWEQRGYFRPGGGATPYCIVIPPPNVTG
                     SLHMGHAFQQTIMDALIRYHRMRGHNTLWQGGTDHAGIATQMVVERLLAAEGTDRLTL
                     GREEFTRRVWDWKEHSGGTITRQQRRLGNSVDWSRERFTMDPELSEAVREVFVRLHGE
                     GLIYRGQRLVNWDPKLHTAISDLEVVQEEEDGSLWHLRYPLEDGSGQLVVATTRPETM
                     LGDTAVAVHPEDERYRNLIGCKVRLPLTDRLIPIIGDDYVDPAFGTGCVKITPAHDFN
                     DYEVGKRHELPRINLFTIDAELNENAPAPYRGLERFAARARIVADLEALGLLEKIEKH
                     RLKVPRGDRSGVVIEPYLTDQWFVATKALAEPAIRAVEDGRIEFVPKQWENTYFAWMR
                     DIQDWCISRQLWWGHQIPAWYDATGNVYVGRSEEEVRAVHDLGDMPLRQDQDVLDTWF
                     SSALWTFSTLGWPRDTPELRTFHPTDVLVTGFDIIFFWVARMVMMTLKFMDEVPFRKV
                     YVTGLIRDEHGQKMSKSKGNVIDPLDLIDGIGIEELVAKRTAGMMQPQLAEKIARATR
                     REYPEGIRAHGTDALRFTLCALASTGRDIKFDMGRLEGYRNFCNKLWNAARFVMMNTE
                     GKDCATAPDADFTLALPDRWILSRLQQTIAEIERAIAGFRFDLASQALYEFTWNDYCD
                     WYLELSKPLLAASAEPRQARGTRRTLLGVLETVLRLAHPFIPFITEEIWQRVAPLAGR
                     AGATIMLAPYPQAEVSLLDEAASADIEWLKQVIVAVRNIRGELNIAPGRQLPLLARNG
                     SDEDRRRMTEHRDLLHALAKLGGSTWLDGEEAPPSAAQVVGQLELLVPLAGLIDVAAE
                     RGRLAREGDKLGKEVERLAAKLDNPGFTAKAPAEVVAKERARLAEISAQRDTLLAQLE
                     SLQGVS"
     gene            complement(14342..14698)
                     /locus_tag="PLW13_01025"
     CDS             complement(14342..14698)
                     /locus_tag="PLW13_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68980.1"
                     /translation="MTETTPEHSDPRRGLLDELQSLSATLDPSAHDAPTYGDIPVLQD
                     VVELPRAAQATPGLVSAAFAPGIDLGAALEVEARSIVDDLMDEFLPLLEAQLRERLEA
                     RMRQLLADKAGPATPG"
     gene            complement(14679..15140)
                     /locus_tag="PLW13_01030"
     CDS             complement(14679..15140)
                     /locus_tag="PLW13_01030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HQY68981.1"
                     /translation="MTRIDFYLLPVSEPHGKLSFACRLAEKAWQSGHQVYVHASDTAE
                     AGAIDALLWSFRDTSFVPHRLLGAAEGEPAPVEVGSGEAAADHHDVMINLSAAVPPLF
                     SRFERVAEIVLNDPHALAASRQRWSFYKDRGYQLQHHDMQHLRSAGDRDNA"
     gene            complement(15154..16659)
                     /locus_tag="PLW13_01035"
     CDS             complement(15154..16659)
                     /locus_tag="PLW13_01035"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQY68982.1"
                     /translation="MDISIKHIADPAAERCGCLVLPVADGGKFGTMAARLDQATGGQL
                     ARLVKTEHFSGAAGKSVLLHHPAGIAAQRLLLLGAGKPEAADAGAFRKMVMHALGAVQ
                     ATGATDLVMLIDDVVTNERDRDWQAQQLGLLAGAAGYRYTHTLSQPEPGSRLKKVVLG
                     SATRPSAASVRALAEGRAMAAGANLARELGNLPANICTPSYLASQARALAAQHAQLHC
                     KVLDEKKMAELKMGALLSVTAGSKEAAKLIVLEYRGGARGARPHVLVGKGITFDSGGI
                     SIKPGAEMDEMKFDMCGAASVLGTMRAIAELKPRLNVVGIVAACENLPSGTATKPGDV
                     VKSMAGITIEVLNTDAEGRLILCDALTYAERFEPQSLIDIATLTGACVVALGKHASGL
                     FSNNDELAAELLAAGSDTHDRAWQMPLWDDYQEQLKSNFADVANIGGPGAGSVTAACF
                     LSRFTKKQRWAHLDIAGSAWDQGAAKGATGRPVALLTRYLLDRARHHKAAD"
     gene            complement(16752..17231)
                     /locus_tag="PLW13_01040"
     CDS             complement(16752..17231)
                     /locus_tag="PLW13_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQY68983.1"
                     /translation="MTRSPAGSPDALPAPGLLRRFAAMLYDSLLLFAVGWTVTAVAVG
                     LRAASVGEAAIRASGKAAVSGLLLQAAIAATTALFFGWFWTRSGQTLGMQAWRLRLQQ
                     PDGSPISWPQAMIRMGGAALSALCLGAGYWWALFDREKRTWHDRLSGTRVVLLPKRG"
     gene            17431..18123
                     /locus_tag="PLW13_01045"
     CDS             17431..18123
                     /locus_tag="PLW13_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY68984.1"
                     /translation="MRILVIEDDASVLDYISRGLRESGFTVDQAADGKDGLFRASAEK
                     YDALVVDRMLPGVDGLAIIRTLRGADNNTPALILSALGEVDDRVKGLKAGGDDYLVKP
                     FAFAELLARIEALLRRKDSSSAVTRLRVADLEMDLLSHKVTRAGKSLNLQPREFRLLE
                     YLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRQKLDKEFDKPLLKTVRGAG
                     YMLDEPEQDFPG"
     gene            18095..19486
                     /locus_tag="PLW13_01050"
     CDS             18095..19486
                     /locus_tag="PLW13_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQY68985.1"
                     /translation="MNPNRIFRASLFRYTLNYLVGVSVAVFVVLAVGYASLTLSYFRD
                     MNALIGAETRELEARFATEGARGVSVVIADRIQASGFPRLFYLLVDAEGQKVAGNLSR
                     WPERLGQGEEWLGFDRTLAEISGGEQEFDFVGASTRFADGSRLLVARHYKDISEFLRI
                     TLTVLVQGMLATIVLGAVWAAILALILERRIGAINDSIAGILSGDLSQRIPVARGGDD
                     DDFKRLIVNLNHMLDRIAQLMEDLKQLSDNIAHDLRTPLTRLRNRLASIEFDGQGGRN
                     EAVAALLQESDELLATFNALLRIAQIESGNRRSGFAATDLVTILRDVCEFYEPLAADK
                     EQRVSVVLPPSCPGVFDRDLLFQAFANLLDNAIKYAPAGGHIEVRLDAGTDVATISVA
                     DDGPGVPAAERDKVFQRFYRVEASRSGHPGNGLGLSLVQAVINLHGGGVQLGDNAPGL
                     RVSVWLPRAAAPA"
     gene            complement(19492..20178)
                     /locus_tag="PLW13_01055"
     CDS             complement(19492..20178)
                     /locus_tag="PLW13_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68986.1"
                     /translation="MSDERFDIVFGGELLTGFAPATVADNLARLFKTSPETVARLLDG
                     GMHTLKRNVDAATAAKYRSAMERAGARATLRPLAPDSAAATRAAGLDLAPPGGELLAA
                     HERRPATAIEVDTSHIRLASAFATTPEEERPLPVVPDTSHISIAAVGADLLAQREAAA
                     PAPPPDTSAITLAEPGAVLGPQGAALAVAVPDISHISLAPPGAPLEELRPERRTVTPD
                     TSALELAPPG"
     gene            20295..21383
                     /locus_tag="PLW13_01060"
     CDS             20295..21383
                     /locus_tag="PLW13_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY68987.1"
                     /translation="MPDTVTQDVLPNELVPTQFAASLARMLRERGLDPAQSTLAAGIG
                     FDVLDPQAAAYRSEIPALAYSRLYRSVMTALQDETFGLRRDVGMRPGAFRMMCYCLLG
                     CANLGRAIARICEFHQVFYDGQLEMRLKREGESARFEIVVPPQANALAAAGDTAFLMS
                     PAAAACGLSMWHRFFGWLVGHPLPLDRVELSGSPPANLARYRQLFNCELQFGVVIPAF
                     AFPADYLAYPLVHNEDSLRDFLRTAPYQLLTMPADPGKQSLVDQVRALIGHDFSRGFP
                     SFERITELLHMSAPTLRRRLKREGTTFQELKDSCRREASIDYLNRPELSITDVAILMG
                     FTDPSAFYRSFKKWTGMTPGEHRALEAR"
     gene            21541..22743
                     /locus_tag="PLW13_01065"
     CDS             21541..22743
                     /locus_tag="PLW13_01065"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQY68988.1"
                     /translation="MAHEALIFDAIRTPRGRGKPGGSLYEVKPIDLLVNLMEAMKQRN
                     DLDTSRVEDLVIACGEPVGEQGQDIARTAVTYSSWGEATAGVQIHRFCAGGLDAVNMA
                     AAKVAAGFEDLVAAGGVESMSRLGIGASGGAQGDPWVAMRTLFVSQGIGADLVATLNG
                     FSREDVDRYALMSQQRAAAARAEGRFRSIVPVRDMNGVTILDHDEFIRPGTTLADLAR
                     LKPSFAQFGEWGQDELLKLRYPQLESVEHVHTPGNSSGIVDGAGLVLIGNERAGRELG
                     LKPRARIVATALVGTEPTIMLEGPAPAARKALAKAGLKVEDIDLFELNEAFAAVVLRF
                     QALMGVPDERINVNGGAIAMGHPIGATGAMILGTLLDELERRNLERGLCTLCAGGGIG
                     IATIIERV"
     gene            22788..24941
                     /locus_tag="PLW13_01070"
     CDS             22788..24941
                     /locus_tag="PLW13_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY68989.1"
                     /translation="MGYITLNKGAEGIVELVFDQPGSSVNTMGLEYDAAMKAAVAELQ
                     GMVAAGGVRGVYVRSGKPGQFFAGGDIKEMLEMDLDVPAERKQQMFEGTLATKAPLRT
                     LETLGVPVAVGINGAALGGGFEIALACHHRIALAGVDLGLPEAQIGLMPGAGGVVRLT
                     RLVGMQEAITLISQGTRLKAEKALEKGLLHEIAASEDEMRARAHAWLLANPGAKQPWD
                     SEGYKIPGGGPDHKANQGLMMLGPVNVMNQTRGLMPAQKAIFAAVADCARVDFDTAQK
                     IEARYFLSLLLDQTARNMMTAFFVQMNAIGKGASRPAGIARRKVKTLGILGAGQMGAG
                     IAYAAARKGIAVFLKDMDLAAAERGKDYARQACAKNKRMSDAEREALLAMITPTADYT
                     DLAPCDAVIEAVFERRDVKAAVVRELEAVLGHTALIASNTSALPITELAEASVRPANF
                     IGMHFFSPADRMPLVEIICGEQTGDEALAAAFDLAQQLGKTPIVVNDAPGFFTTRVIG
                     KTISQGAQMVLEGIDPVLIEAAARANGSPVGPLAAIDEISQETAYRNGLQMKADAEAR
                     GETWQEQPAGELVRRMVEEFGRKGRRSGGGYYEYPEGGKKHIWPGLKQHFAPNGCKEV
                     PFEDIKDRLVFAQCVEAVRAMQEGVITSVADGNIGSIMGIGFPPHTGGVFQCINAWGL
                     KRFVARARELAQAYGADFEPPRLLLERAETGELFG"
     gene            25038..25325
                     /locus_tag="PLW13_01075"
     CDS             25038..25325
                     /locus_tag="PLW13_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68990.1"
                     /translation="MVRKIPDGKAGGADSMRPSRTDTESIFGDKRSGWDRRKQQVSVT
                     ESRREVSDRRKTGRGAQGAWWLERDYVESHHFVQKSSSSRTRKRDDTPSGE"
     gene            25376..25882
                     /locus_tag="PLW13_01080"
     CDS             25376..25882
                     /locus_tag="PLW13_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY68991.1"
                     /translation="MQPDTRSPSARSCVALLVLLLCCALFARARAGDEDGFRPYDREA
                     DPRAQIAALLEDRSSGKRILLTFGANWCSDSRSLARRYRTPELAAVLEREFRTLHVDV
                     GRSQRNRDIVEQYGNPTDMGIPSVVLLAPDGTLLHTDHGSLSAAGRMSDPELIAFFER
                     LAARYPVP"
     gene            25930..26784
                     /gene="trxA"
                     /locus_tag="PLW13_01085"
     CDS             25930..26784
                     /gene="trxA"
                     /locus_tag="PLW13_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQY68992.1"
                     /translation="MAELMVEITRDNAQRMLIEESHARPVLIDFWADWCAPCKALMPI
                     LDKLVREYAGQVMLAKVNCDELQPIAQQFGVRSLPTVILMKDGQPVDGFVGAQPESAV
                     RQMLEKHLPKPWDILLHQALELLEAGDAAGALAPARSAYEESRQRADIAKVYADILIE
                     LNRVDEAQVVLDSIPMADQDGDFERLLSELQLKKQAADTPEIQALLQARARDPDNMDT
                     GYQLAVQFSQAGRSREALELLIGILGRDREFRGGEARKTLLDIIRALGKGDPLAAEYQ
                     RKLFALMY"
     gene            complement(27371..28711)
                     /gene="yegQ"
                     /locus_tag="PLW13_01090"
     CDS             complement(27371..28711)
                     /gene="yegQ"
                     /locus_tag="PLW13_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HQY68993.1"
                     /translation="MRKPELLSPAGTYKSMQYAFAYGADAVYAGQPRYSLRVRNNDFS
                     RIERLAEGVDYAHRLGKQFFIASNVSPHNDKLKSYLRDMEPVIAMKPDALIMADPGLI
                     MMVRERWPEIPVHLSVQANAVNWATVKFWKRMGLTRIILSRELSLEEVAEIRQECPDI
                     ELEVFVHGALCIAYSGRCLLSGYITHRDSNQGACTNTCRWKYQAHEAVQTESGDVVAI
                     HEPVPLQAPEPEPAPVQDRIYLLQEEGRPGEYMPAYEDEHGTYIMNSKDLRAIQHVRQ
                     LIDIGVDCLKIEGRTKSHYYVARTAQAYRRAIDDALAGREFDMGLMEELEKLASRGYT
                     EGFYRRHPPGEFQNYERGVSKGDYQQFVGEVLDLDAASAMLTVSVNNRFALGDRLELM
                     TPQGNCEFTLTDMTSEAGERREVAPGSGHVVKFPLPEAARELRDAGYAMVARYI"
     gene            28818..29138
                     /locus_tag="PLW13_01095"
     CDS             28818..29138
                     /locus_tag="PLW13_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HQY68994.1"
                     /translation="MRMQQTIERKLAEAFAPGELEVLNESHQHSVPPGSETHFKVVLV
                     SAAFEGKRSVARHQQVYKVLAEELARGVHALALHTYTPAEWAQRAAHAPDSPACMGGG
                     KHDH"
     gene            complement(29132..31255)
                     /gene="ppk1"
                     /locus_tag="PLW13_01100"
     CDS             complement(29132..31255)
                     /gene="ppk1"
                     /locus_tag="PLW13_01100"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQY68995.1"
                     /translation="MPTRHKTLPLYPKELSWLSFNARVLQEAEDPEVPLIERVRFMGI
                     FSNNLDEFYRVRYAEVRRLAAFAKGREKARWEKLLDDIRDAVGDLQMRFDRVYRTCLS
                     QLRENNIYLVDERQLDAQQREFARQYFFARVMPELAPIIISDATATPQLEDGSIYFAV
                     RIQLRNQSIRYAIVNIPSDRLPRFIVVPSPAAQSSRQVIVVLDNIIRACLPQVFQGVF
                     DIERAEAFTFKITRDAELELGEGITQSLVDALSSSLKKRKLGDPVRLVYDRRMPNDML
                     DIMVKRFRLGSYDNVLPGARYHNSKDFMEFPNLGARSLEHRPIGPLMVPRIDRHANIF
                     AAIAERDVLLNYPYHSFRYTEQLISAAAIDPAVRAIQVTLYRVARNSHIGNSLICAAL
                     NGKEVFAIIELRARFDEAANIDWAKRLTEAGVHVVFGIPGLKVHSKLILISRQEGTQL
                     RRYVHIGTGNFNEKTARFYTDLALFTANQEIAEDVHHVFDFIRHTYRRHKFRHLAVSP
                     LTNRSTFLRLIHAEVLNARAGKPATIFLKCNNLVDEELIDRLYEASQAGVRIRVIVRG
                     MCSLLAGVAGVSDNIEIISIVDRFLEHSRVYVFGNGGEPRYFISSADLMTRNLDHRVE
                     VTAPVYDKLAQRRLQRLLETQWADNVKARVLSADQDNGYRDRGHKRRLRSQEVLARYY
                     ADDQRRETLALEQPEAGEDGPAKAQ"
     gene            31408..32901
                     /locus_tag="PLW13_01105"
     CDS             31408..32901
                     /locus_tag="PLW13_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HQY68996.1"
                     /translation="MNESSGPVCAVLDLGSNSFHLLIGRLAGGRLVTLDRRKDMVRLA
                     QGLEGDGQLSEPVMSRALDSLAVFSQQLRNIPRANVRVVGTNTLRAAHNADVFLERAE
                     KIIGVPIDVISGQEEGRLIFLGVAKGHASNAIRRLVIDIGGGSTEFIVGRRKAKRVES
                     LFIGCVSMSERFFPERRFTREGYQRALTLARAEIQHLADGYGTARWDEAVGTSGTIRY
                     VEAVIDRLTPCDHLITREGIERVVDHLKRSKKPDALQSLGLSPERVPVFPGGLAILHA
                     AFIELGIERMHVSDYALKEGVLYELADRGEHEDTRRQTMDYLTKQFNIDRRQAARVER
                     LARSLLPQVAPQLTVERAFAQQVLVAAARLHELGLAVSHSGYHKHGAYVLEHADMPGF
                     SRREQMMLSFLVLNHRRKLRAPEVSSYSFRPDWALVLVLRLATLFNRMRIDQRMPSIR
                     LAPMRKGWSIELPSGWLRKHPLVEEDLRAENEFLAAIGSGLQVKTRR"
     gene            32912..33997
                     /gene="truD"
                     /locus_tag="PLW13_01110"
     CDS             32912..33997
                     /gene="truD"
                     /locus_tag="PLW13_01110"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF002153.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HQY68997.1"
                     /translation="MSFTAALPDIEAARPRAGRPAGQGLIRQQTTDFTVEELIEMDLD
                     EGGEHLWLRVRKTGENTEHVARLLAQAAGIRPQQVGYAGRKDRHAVTEQWFSLHLPGR
                     PDPADFALPPSVEILGMRRRRRKLAVGGLRGNRFRLLVREFAGDAALLDERLRETAVR
                     GAPNYFGAQRFGRGGGNVAQALAWMRGERRVDDRKLRGLLLSAARSQLFNALLARRVQ
                     DGSWCEARAGDALMLDGRGSFFVAGEIDATLRERVARGELHPSGALWGRGELATRSGI
                     AGLERAVATEHAELATGLERAGLEQERRALRVMPRELTWGWIDDTTLAIAFRLPAGCF
                     ATTVLDDVLDCRDAAQAAVARPDGAGD"
     gene            complement(34009..34935)
                     /locus_tag="PLW13_01115"
     CDS             complement(34009..34935)
                     /locus_tag="PLW13_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY68998.1"
                     /translation="MNTAAIRLDDYAFLQVEGAKALPFLQGQLTCNVQDAAPAGVLPG
                     AYCTPKGRVIASFLLWARSPDSVLLRLRADIADDTATVLGRYAALSRVRVARCDMACI
                     GLLGTGLETELHTLFPDWPAQPRALGAAGAWRLLRRDGEPAQFEIWAPRSETGALWES
                     LASRLPVANTERWRLALVRAGAAEIQHATVDQFLPQMLGYDTSGAVSFRKGCYTGQEI
                     IARTHYKGSVKRRLHRLGGAGPACPAVGDELRHRDADRVVGSVVESAMDGASRFELLA
                     VLSDEALDGANCLAVTARGEALEVLPLAERAS"
     gene            35061..35339
                     /locus_tag="PLW13_01120"
     CDS             35061..35339
                     /locus_tag="PLW13_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQY68999.1"
                     /translation="MDEAAENRRVYWHSRRGMLELDLVLMPFAEQVYPGLGEDMQRRY
                     RALLECEDADLFAWIVHRGEPDDPELKVIIARVLGNGMPRPELPPGAV"
     gene            35299..35718
                     /locus_tag="PLW13_01125"
     CDS             35299..35718
                     /locus_tag="PLW13_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69000.1"
                     /translation="MECPGQSFHLAPSRRLLYALCVLHLAALAGLVATALPSALMLAL
                     ATLLLASLARHWRAHGALERRGGTRLEFRGEAWWLHGDGAPQPVRLRADSAVLPFLVV
                     LRVQQARRVRSLIVLGDSLPRGELRRLRVLLRLRGAR"
     gene            complement(35734..37311)
                     /gene="nadB"
                     /locus_tag="PLW13_01130"
     CDS             complement(35734..37311)
                     /gene="nadB"
                     /locus_tag="PLW13_01130"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQY69001.1"
                     /translation="MPQRYTHDVLVIGSGAAGLSVAIQVADRARVAVICKGELGDGST
                     SWAQGGIAAVLDDEDTIDSHVADTLRAGAGLCHEDMVRFTVEHSRESIQWLVDLGVGF
                     TKLLTPGGENEFHLTMEGGHSHRRIIHAADATGRAVSDTLIEQARRHASIELFERHIA
                     VNLITTAGRCRGAYVLNKDTASVDLFSARFVVLATGGASKAYLYTTNLDTATGDGIAM
                     AWRAGCRVANMEFNQFHPTCLYHPSAKSFLISEAVRGEGGVLLLPNGERFMPRFHPQA
                     ELAPRDIVARAIDHEMKRLGIDCVYLDISHKGEDFVRSHFPTIYSRCLELGLDMARAP
                     LPVVPAAHYTCGGVMIDRAGQTDLPGLYAVGETSFTGLHGANRLASNSLLECFVFARA
                     AAQHILAQGESIAISAGLPDWDASQVTDSDEDVVISHNWDELRRFMWDYVGIVRTTKR
                     LQRALSRAHLLKQEIAEYYSNYRITSDLVELRNLALVAELIIRSALGRHESRGLHYTL
                     DHPGLAAEAGDSVLTPG"
     gene            37520..38104
                     /gene="rpoE"
                     /locus_tag="PLW13_01135"
     CDS             37520..38104
                     /gene="rpoE"
                     /locus_tag="PLW13_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HQY69002.1"
                     /translation="MFSGEEGDQQLVERVQKGDNRAFDVLVLKYQHRIYSLVTRFIRD
                     PDEVQDVVQEAFIKAYRALPGFRGESAFFTWLYRIAINTAKNYLVSRARRPPGADVDV
                     EDAEHLESGAALRDLAGPENQLMTEQLRAVIDRAIRALPEDLRTALTLREFEGLSYEE
                     IAEVMRCPVGTVRSRIFRAREAVDHEILPLIEGN"
     gene            38156..38797
                     /locus_tag="PLW13_01140"
     CDS             38156..38797
                     /locus_tag="PLW13_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HQY69003.1"
                     /translation="MSQLNAPLREMISAVVDGEASEFEYRRVLESVDDAGVTALLGRH
                     YAVRAVARGEARALCPPALTAGILAALAEQQPAAVTAPSARRWRMPVGGVAVAASVCM
                     AAVFGLRALAPEPAGMPQVAVVASSSSAGASLGELGRPGGLPVPVTGPAVPVGFGAAV
                     PAPGGALQHAGANPDQLAEQRLRLFMADHVQNAALNTNQGMLPYARVVSYEAP"
     gene            38817..39779
                     /locus_tag="PLW13_01145"
     CDS             38817..39779
                     /locus_tag="PLW13_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HQY69004.1"
                     /translation="MRPLAVLACLLPGFLSVPLHAVEAGDPASRLDAMSRSFRSLDYH
                     GVFTYEQGQSLSSVRIAHAVVDGVEQERLVHLDGEQREFVRRGHRVDCEHAGDRLMRL
                     DPAARFAQRAQTGVAGAQLGDHYAIEFDGSERVANHRGQRIRIVPRDPYRYGMNVVLD
                     EASSLLLKSETTDGAGRVLERFQFVDLQIGAPVAAAELAPETPGAHHAAGHAGAEEPA
                     PFAWTVAWLPEGFVATGREVRSARAGGPAVEVQSYTDGLAVFAVFVERTAERPPHAGQ
                     ASRGATVSYVVPRDQEHLVTVVGEIPVETAQLIANGVNFPDATP"
     gene            39776..40237
                     /locus_tag="PLW13_01150"
     CDS             39776..40237
                     /locus_tag="PLW13_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQY69005.1"
                     /translation="MMIERARVVAVEADACWVETIARPGCGACASGQGCGGGVIGRLL
                     GERRHHLRLVLPAGLSPAVDDEVEIGVREHSVLRASVFAYLLPLAGIVAGAVTGERLA
                     PPGSAEAWSMAAAAAGFLLAVAAARALSRRPRAGVLEPELLRVFPAARSLA"
     gene            40313..>41108
                     /locus_tag="PLW13_01155"
     CDS             40313..>41108
                     /locus_tag="PLW13_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY69006.1"
                     /translation="MLALAGKSWSAALPDFTTIVEKNSGAVVKIMSTQKSPEVEGLPP
                     DIDPRALEQMPEIFRHLFQYRGQPQRERQSMGSGFLVSADGYILTNHHVVDGADAVTV
                     RLADRREFEARIVGSDKRSDIALLKVEARDLPVVSFGNTDELAVGEWVVAIGSPFGLD
                     YSVTAGIVSARGRSLPSDGETGNYVPFIQTDVAINPGNSGGPLFNLDGEVVGINSQIY
                     TRSGGSIGLSFAIPISVAIDVMEQLKASGHVVRGWLGVGIQDVDRAL"
BASE COUNT         6347 a        13578 c        14505 g         6678 t
ORIGIN      
        1 gcgcgacgat cagcgagaaa tgcttcgcca gcccgtgcat cagccgtgtg agctcggccg
       61 ggctgaggaa ggtcggcgtg ccgccgcccc agtgcagctg ggtcacgcgg cggcgccggt
      121 cgaaatggcg cgagagcagc gcaatctcct tctccaccgc gacgatgtag cgctcggcaa
      181 cggctgcatc gcgggtgacg atcttgttgc aggcgcagta gtagcagatg ttgcggcagt
      241 acgggatatg cacgtacagc gacagcgggc cgatgaccgg gggacgctcg agcaggtact
      301 gctcgtattc gcgcgcgccg aaaccgtcgg tgaactgcaa cgcggtcggg taggacgtgt
      361 agcgcggacc gggcacgtcg tacttgcgga tcagggcggg atcccagcgt atgcggtcgt
      421 cgaatccgtg cctggcgagt tggatgtcca tgttccgtct tccgtaatcc gtgcgtgtcg
      481 cgcctcgggc ggggcctgcc attgccgagc agtttactcg gcgattcccg gggcgcgttg
      541 atgcacgtca acgcattgcg cgggcgggaa tattgccgtg atctgacggc gtttcgtgaa
      601 tcttgacgaa ggtcaaatcc gtattgtcgg aggcgactac aattgcgccg tggtttttga
      661 gtgttggagt aaacaggagt gcaaccatga agaaagtaac gaaaatcagc ctgctcgcag
      721 cggcgatgct ggtatcggga gccgcgagcg cgtacgaggc gggcgactgg atcgtgcgcg
      781 gcggtctcgt gaacgttgat ccgaatgacg acagcagcaa cctcagcgtc gagggcagcc
      841 gcctcggcgg cacgcaggtg gacgtggacg cagacacgca gttgctgctg aacgtgacct
      901 acatggcttc gagccatgtc ggggtcgaac tgctcgcggc aaccccgttc gagcacaccg
      961 tcagcaccgg tggcctgagc aggtacggcc tgaatgatgt cgacctgggc gagatcaagc
     1021 acctgccccc gacgctgagc gcgctgtggt acccgatgcc ggccagttcg aagtttcagc
     1081 ccttcatcgg cctcggtgtc aactacaccg tattcttcga cgaggatgtc agccgtgaag
     1141 cgcagaatgc gctcggtgcc gacaatctga gtctcgatga ctcgtggggt ctcgctggtc
     1201 gcgtcggcat ggactacatg ctcaacgact gctggtcctt gcatgccggt gcctactaca
     1261 tcgacatcag caccgatgcc gaggtggaca ccgcgctcgg cgcggcaaag gtggacgtcg
     1321 acgtggatcc ctgggtctac acgctgggcc ttggttaccg cttctgatcc atccgctcag
     1381 tggtgatggt gctgggcgtg aggatcctgg ccgtgcggca acgcgatggc cagggtccac
     1441 accccgaaag cgatcaccag cagtcccgcg attacccgta atccgcggcg ttgcagcagc
     1501 gccctgagtt cattcgccag ggcgccgctc gcgacgactg ccggcaaggt tcccaggccg
     1561 aaggccagca tcagcagcgc accccgcgcc gcgctcccag aggttcccgc ccaggtcagc
     1621 gtgctgtaca ccaggccgca gggcagccag ccccaggcca tgcctgccaa cagcgccttg
     1681 tgcagccggt cgaccggcat cagccgtgcg gccatgccct gcacgtgccg ccacgcaccg
     1741 tgacccgcgc gttccagcag cgccagcgcg ttcagccagc ccgccagata gcagcccatc
     1801 agcaccagca gcacgccggc gacgatgcgc agcacgcgca tccccccgtc gcccgcgagc
     1861 ccgccggccg cacccgcgaa gagcgccccc atgacggcgt agctcatgat gcgcccgagg
     1921 ctgtagccgg tgagcagcat gtagcgctgc gcggggcgca ggtccgggcc gagcgcgaag
     1981 gccagcgcgc cgctgatgcc gccgcacatg ccgatgcaat gcgtgctgcc cagcaggccc
     2041 agcagcagcg cggaaagtat cccgggatcc ggtgtcaagg acgttctccg tccgcggcgg
     2101 gcggcggcgt cgcgggcgct tcgtcgtttg gcgccacggt gtcggtatcg tcgaacagga
     2161 tgctctgcga ggccttgtcg agatcgtcga actgtccgct gttcaccgac cacaggtagg
     2221 cggcgacggc cagcccgagc aggatggtcg tgaccggtat cagtacgtag atgctatcca
     2281 ttggcgcccg cgggcctgag cgaagtgctg gcaggcgcat cgttgcgcag gcgcagcgcg
     2341 ttggcaatca ccaccagcga gctcgccgac atgccgatgg ccgccagcca cggcggcacc
     2401 aggcccgcgg cggcgaacgg caccgcggcg aagttgtaca cgatggccca ggcaatgttc
     2461 tggcggatga cgtgacgggc gcgtcgcgcc atcaggatcg cgtccgggat gcgccgcaga
     2521 tcgcccgcga gcagcatgca gtcggcgttg gcacgggtga cgtcggcggc gttggccatc
     2581 gcgatcgaga ctgcggcacc ggcgagcacc gcgacgtcgt tgatgccgtc gccgaccatc
     2641 accacgcgcc gcccgctcgc ctgcagctca cgcacggcag cgagcttctg ctcgggaacg
     2701 cagccggcgc gccattcatc gacgccgagc tgcgtggcga cgcgttgcac ctcgcccgcc
     2761 gcgtcgccgc tgagcagtgc cacgccgagg ccggcccggc gcagcgccgc gatcgccccg
     2821 gtggcgtcgg cgcgcagggt gtcggtgagc cggaaccagg ccagggcctg cccgtcgcag
     2881 gccagcagga tccatttgcc gacctctggc ggggcgggcg catcgcctgc cagcgcgaag
     2941 cccggacgtc ccagccggta gcatcggccg tcgatcacgc cttccagccc gccgccggga
     3001 tgggcgcgaa aggcctccac cgggtcgcgc gcctgctccg cgaaggcggc ggcgatcggg
     3061 tgctcggaca gtgcctcgag gctcgcggcg atgcgcaggc agctcgccgc gtcgcgctcg
     3121 ccggttgtgc gcacctcggc cagtttcacc tcgccggtcg tgagggtccc ggtcttgtcg
     3181 aataccacgg tatcggctgc ggccagcccg tccagcgcat ggccgcgggt gaccaggaag
     3241 ccgcgcgcgc gcaacgccgc tgtggctgcc gtcatcgcgg ccggtgtcgc gagcgacagg
     3301 gcgcaggggc agctcaccac cagcaccgag agcacgatct ccacgacgcg cgccggctcg
     3361 tgctgcagcc accacagccc tgtcagcacg gacacaagta tcaccgcgag cacgaaccag
     3421 cgcgaagagc ggtccgccag ctgcacgatc gcgggctttt cctgctgcgc gcgatcgacc
     3481 agcgcgagaa tcgcgccgag gcgcgagttc attccggtcg cctgcacctg cacggtgagc
     3541 gagccgtcgg tgttcagcgt gccggcgctc accctgtcgc cggcggcctt cgccaccggc
     3601 agcgactcgc cgctgatgct cgactcgtcg acgctgctct cgccgccgag cacggcgccg
     3661 tcggccggga tcgcctcgcc gggccggatc aggaccaggt cgccgggacg caacctgccg
     3721 acgggcacca gttcgtggcg ttcgccgccc tccagcacgc gcgtggcggc ggccggcacc
     3781 agcgaaagca tggtgccgcc ggcgagtccc gcccggtgcc gcgcacgcat ttccaggtag
     3841 cgccccagca gcacgaagaa ggtgaacatc gccaccgagt cgtagtagac cgcgccgccg
     3901 cccgcgacgg tgaaccacac gctggcaccg aaggcgagcg cgatcgcgag cgcgatcggc
     3961 aggtccatgc ccggggcccc gtggcgcagg ccgcgcagcg ccccgctgaa gaagggcgcg
     4021 ctggagtagg cgaccaccgg cacgcagagc accaggctcg cccagcgcag gtaggcgcgg
     4081 taatccgctt ccatgccggc gaaatcgccg gcgtagagcg cgatcgcgaa catgccgatc
     4141 tgcatctgca cgatgcccgc gaggcccatg cgctttagtg cgctgcggtt ctccgcgcgc
     4201 tgggtctccg cgcgcccgga ggcgaggaaa gggtagggac ggtaacccag ctcgcggatg
     4261 cggcccatca gctggctgag cggcagctcc cccgggcgcc agcgcacgct ggcgcgctgc
     4321 tctgccaggt tcacgctgat ggccgccacg ccgggctgac gctgcaggtg atgctcgatg
     4381 agccaggtgc aggcggcgca gttgatgccg tcgatcgcga gctgggcgcg gcaatagccg
     4441 tcatcctcgt gcagcacgaa gtcgccctgc gcatcctcgg catcgaaaac ggcgtactcc
     4501 gcggcaccgg cacccgcatc ggcaggagcc gcggcggcag tgtcacgatg acggtagtag
     4561 ccagcgagac cgctgccggc aatcaggctc gcgaccgccg cacagccggc gcagcacatc
     4621 gcgcgggatt cgccgtcgat gtcggctagg aagcggctgc cgggaggcac cggcaggccg
     4681 cagtggaaac aggcgacggt catgtcctca gtcggcgtcc aggcgcaggc ttgtgacgga
     4741 ttcgctgcct ggaacgaacg taccgcgcag gcgccacgcg ccctgcggcc catcctcgtg
     4801 ttccagcagg atgtagcgtt tgccgtcgag cgccgcgccg accttgccgc ggtagagtcc
     4861 gtttcccgcc gcctgcagca ccaccgcctg gtcgcgctcg tcgtcggtcg gatgcacgat
     4921 ggtcagcgtg agttccggtg ccggggcggc ggcgatttgc agttcgatcg cgagcgtggc
     4981 ggcgtcgtag cgcagttggg cctcgatgcc gcgcgcacgg gcgttggcct cagcctccag
     5041 gcgctggttg atcgccagcc cctccttgta atagtcgtcg cgcaccaggg cctcgtcggt
     5101 gctcagcgcc aggtagagcg tcacgaagca ggcgatcacc accgtcagcg gcagcaggat
     5161 caggaaccac ggccaaccct ggcggtacca cggggcggat tcgttcatcg cgcaggaccc
     5221 gggccgagga agcggctctc ggcggtgtct tggatcccgc cctggccgac ggccttgacg
     5281 gtgaaccgca cttccgcgtt gctgttgccc tgcaggctct cgcgtggcac ctgcagcgcg
     5341 atgggcaggg ccagcacgtc accggactgt atggtcacgc gggtcggccc cacgatgctg
     5401 gcgccctcga taccgctggc cgcgatgcgg tagctcattt ccttctcgcc catgttgcgg
     5461 atcttcagcg tgtagacgtt ctcaaccaga ccgccgtcga cttcgcgaaa cagctggccg
     5521 cgctcgcgga tcgcatcgat gcccaccggg atgcgattga acagcacgct gaggaacagg
     5581 ctggtcagta ccagcacggc gagcgaatag ccggcgaggc gcggacgcat gatgcggctg
     5641 ggcttgccat cgagcgcgtt ttcggtggtg tagcggatca gcccgcgcgg gtagttcatc
     5701 ttgtccatca ccgagtcgca ggcgtcgatg cacagcgcgc aggtgatgca ctcgtactgc
     5761 aggccgttgc ggatgtcgat gccggtcggg cagacctgca cacagagctc gcagtcgatg
     5821 cagtcgccga gggcgagtgc tttcgggtcc gcctcccgct tgcgcgatcc gcgcggttcg
     5881 ccgcgcttgc ggtcgtacga gacgaccagc gtctcgtggt cgaacatcac cgactggaag
     5941 cgcgcatagg ggcacatgta catgcacacc tgctcgcgca gccagcccgc gttgatgtag
     6001 gtggccaacg taaagaacag cgtccacagg ttggcccaca tgcccgcctg cccgagcggc
     6061 agatcgatga ccagcgcgcg gatgggggtg aaatagccga cgaaggtgat gccggtaaac
     6121 gccgcgaagc cgaaccacat cccgtgtttc agcgacttgc gccagacctt gttgaccgtc
     6181 cacggcccct ggtcgagctt gatgcgctgg ttgcgcgtgc cttcgctgac ctgctctgcc
     6241 cacatgaaga tgctggtcca cacggtctgc gggcaggtgt agccgcacca cacgcgcccg
     6301 gcgaagaccg tcacggtgaa caggccgaag gcggcgatca tcagcagcca cgcgagcagc
     6361 gggaagtcct gcggccagaa ggtgacggca aagatatgga acttgcgcgc gggcaggtcg
     6421 aacaggatcg cctgccgacc gtcccagttg aaccacggcg cgccgaaata gaggatcagc
     6481 agcggccagc ccgtgtagag ccgcagccgc tggaaaaagc cctcgacctt gcgcgtgtag
     6541 atacgctcgc gcttctggta gaggtcgatc tccccgacct cggaaggcgg ggttacctct
     6601 atcaccggta tccgttggtc cgtcatcgcc gtaccgctcg cgtgagtgaa aaaaatcgcg
     6661 ccggcagagc cggcgcggtt cgggctgggc aggcggcgtc gtgcgggcgc cgccgcgccg
     6721 tcattggctc gacaggctgt agacgtaagc cgtcagcagg cggatcttgt cctcgctgag
     6781 ctgctcgccg aaggcgggca tctggccgtt gcggcccttg cgcaacgcgt gcttgatcgt
     6841 ttccaggtcg ccgccgtaca gccagatgtt gtctgccagg ttgggcgcgc ccagcatctg
     6901 gttgcccttg ccgtcggcac catggcaggc cacacaaaac atcttgaact gtgcctcgcc
     6961 ggccgcgctc tcggcactgg cgggctcggc acccgacagc gtgcgcacgt aatgcgcgac
     7021 gttggtcacg ccctggtccc cgagtgccgc ttcccacggc ggcatcgccg cctggcggcc
     7081 gttcaccagc gtggcatgga tggcctcggg cgagccgccg tagagccagt ccgcatcggt
     7141 caggttcggg aaaccgacgc tgccgcgcgc gtccgaaccg tggcagacgg agcagttgtt
     7201 ggcgaacagg cgctggccca tgcgcacggc ctgcgggtcg gcggcgatct gctcgatgct
     7261 cttgtcgcgg taggccgcga acaccggctc gaaacgcgct tcggcgcgct gcacctcgcg
     7321 cttccactgg ccctcctggc tccagccgag caggccctgg aagttcccca gacccgggaa
     7381 agccaccagg tagccgacgc cgaaaacacc ggtgatcacg aacatgtaga gccaccatgc
     7441 cggcagcggg ttgtcgtatt cctcgatgcc gtcgtagacg tggcccgtct tggcctccga
     7501 gtccgacggt cgcgagcggt tgccgaacag cagccagtaa gtcccgacga tcgtgatcgc
     7561 cgtgaggacg ataatgtaag cgctccagaa actagtcatc ggatctaccc ttcttggttc
     7621 cgtccaggtc atcgtcggcg aagggcaggt ttgccgcctc gtcgaaccgc tgtttgcgtt
     7681 tgccgctcca ggcccatacg cacacgccaa ggaacgcgat catcgccagt atcgtggcta
     7741 ccccgagcag gtcacccctg tccatcgggg cgactcagcg cgtgctcttg agcagcgtgc
     7801 cgagctgctg caggtaggcc acgacggcgt cgatctcctt gttgccggcc acggctttct
     7861 cggcgccggc gatgtcttcg tcggtgtacg ggacgcccac gagacgcagc gcgcgcatct
     7921 tggccggcgt gtccttgccg tccagcgtgt tctcgaacag ccacgggtag gacggcatga
     7981 ccgattccgg caccacgcta cgcgggtcgt agaggtgcgc ccggtgccag tcgtcgctgt
     8041 agcggccgcc gacacgcgcc aggtccggcc cggtgcgctt ggatccccac aggaacgggt
     8101 gatcgtagac gaactcgccg gcgaccgaaa aatgaccgta gcgttccgtt tcggcgcgca
     8161 acgggcggat catctgcgag tggcagacat tgcagccctc gcggatgtag atgtcgcggc
     8221 cttcgagctg cagcccggtc agcggcttga ggcccgccac cggttccgtg gtctgcttct
     8281 ggaagaacaa cggcacgatc tccaccagac cgccgaagct gatggccacg atgatcagca
     8341 cgatcatcag gccaatgttc ttttcaatct tctcatgctg catgggtcgc gctccttagg
     8401 cgggctgagc ttgcgcgttg gtgacggggg cggtgccggg tgacaccgtc ttccaggtgt
     8461 tccaggccat gatcagcatg cccaggaaga acatcgcacc gccgatcagg cgcacgatgt
     8521 agcccggatg gctcgcgagc acggattcgg cgaagctgta ggtaagggtg ccgtcgttgt
     8581 tgtaggcgcg ccacatcagg ccctgcatga tgccgttcac ccacatcgag gcgatgtaca
     8641 gcacggtgcc gatcgtggcc agccagaagt gcgcgttgat ggccttgatg ctgtgcatgt
     8701 ccttgcggcc gaacagcacc gggatcaggt ggtacatcgc gcctatcgat accatcgcca
     8761 cccagccgag cgcgccggaa tgcacgtgcc cgatggtcca gtccgtgtag tgcgacagcg
     8821 cgttgatggt ctttatcgac atcatcgggc cttcgaaagt cgacatgccg tagaacgaca
     8881 gtgacacgac caggaagcgc agcgtgggat ccgagcgcag tttgtgccac gcgccggaga
     8941 gcgtcatcat cccgttgatc atgccacccc agctcggcgc cagcaggatc agcgacatca
     9001 ccatgccgag gctctgcgcc cagtccggca acgcgctgta gtgcaggtgg tgcgggccgg
     9061 cccacatgta gatcgcgatc agggcccaga agtgcacgat cgagaggcgg taggagtaga
     9121 ccgggcgctc ggcctgcttg ggcacgaagt agtacatgat gccgaggaag cccgccgtga
     9181 ggaagaaacc caccgcgttg tggccgtacc accactgcac catggcgtcg atggtgccgg
     9241 aataggcgga gtaggacttg gtcaggctga ccggcaccgc cgcgctgttg acgatgtgca
     9301 gcaccgcgat cgtgatgatg taggcgccga agaaccagtt cgccacgtag atgtgggaca
     9361 tcttgcgctt catgatggtg ccgaagtaga ggatcgcgta ggccacccat accaccgcga
     9421 tcaggatgtc gatcggccac tccagctcgg cgtattcctt ggaggaggtg taccccagcg
     9481 gcagcgtcac ggcggccagc acgatcacga gctgccagcc ccagaacacg aagctcgcga
     9541 gtccgtcgga gatcagccgt gcctggcagg tacgctgcac gacgtacagc gagctggcga
     9601 agagcgcgga accgccgaag gcgaagatca cggcgttggt gtgcagcgga cgcaggcgcc
     9661 cgaagctgag ccagggcagg tcgaagttca gcgccggata ggcgagctgg gcggcgatga
     9721 gcacgccgac cagcatcccg acgatgcccc agaccacggt catgatggcg aattgcctga
     9781 ccaccttgta gttgtacacc ggatgttcag ttgcgtcggg atgagtcatt gcgctttcca
     9841 gatagtgctg tggttcgagg agtccggccc ccggatggga gaacccgcga tagcgagagc
     9901 gaaaccgtgc caattctcct tgcccgcgcg gcctgcaggc catgacttag gtcaagcttc
     9961 gcgggaaggc gtgacattcc ggggaacttc tgcagctttt gtcactgcga gtggcgtccg
    10021 gttgcccgac ggtggcgacc tcggagcgga tcttagcaag attcagcggc aattcatcgc
    10081 accgggagcg gcgtcattgc acgaacaatt cccctcgtac actgcggttg ctgcgcgcga
    10141 cgcaacgggg gagccgcagc gcgcggcgct gcgctaccat cgcgcggaca acgggtgccg
    10201 ggacggcggg ggcggcggtg atgcggagcg tgatcctgga tgcggcgagc ctgggcgagg
    10261 acgtggacct cggccccgtc ctgtcgcttc ccggtccctg ggtgtgccac gaggcgacgc
    10321 ccgccgcgga ggtcgcgccg cggatccgcg atgccgaggt ggtgctcagc aacaaggtgc
    10381 cgctggccgc agattcgctg gctggcgcgc ggcgcctgcg cctggtcgtc gtgatggcca
    10441 cgggcaccaa caacgtcgat ctcgcggccg ccacgcgcca tggcatcgcg gtatgcaacg
    10501 cgcgcggcta cgccaccgcc tcggtggcac agcacacgat gctgctgctg ctcgcgctgg
    10561 cccgaagcct gctgcccgcc gaacgttcgg cccgcgacgg tacctggagc cgcagtccgt
    10621 tcttctgcgt gcccgggcac ccgacgctgg aactcggggg ccggcgcatc ggcatcgtgg
    10681 gctatggtga actggggcgc gaggtggcgc gactggcgcg cgccttcggc atgcaggtgg
    10741 cgctcgcggc gttgccggga cgccgctacg cggatgcgca ggaccatgcg cgcatgccgc
    10801 tcgcgcagat gctgccctgg gcagacgtga tcagcctgca ctgccccctg accccgatga
    10861 cgcgaggcct gatcggcgca ccggagctcg cgcgcatgcg tgcgacggcg ctgctgatca
    10921 acaccgcgcg cggcggtatt gtcgacgaac acgccctgct cgatgcgctg cgcgccgggc
    10981 gtctcggcgg tgccgcgctc gatacgctgg aatgcgagcc cccgcgtgcc gattcgccgc
    11041 tgctcaacgc cggcttgccc aacctgatca tcacgccaca cgtcgcctgg gcgagccggg
    11101 aggcgcgcca gcgcctggtc gggcaggtcg gcgagatcct gcgcgagtat cgcgccgggc
    11161 gattcatgaa ccgtgtcaac tgatggtgcc ggccgactgc gctcgggccc ctcgcggtgg
    11221 tggaacccct ccaaaaagac gcgcattccc ggatctgccc gatgcggcat gcgctcgacc
    11281 gagcggctgt gctgcccgct gttggcttgc ggggccttgc gccgcaggac gcggcgcgga
    11341 gctacaggga cgtactcgtg cgtgccccgc aggacaatgg cgggcagcgc agccagcgac
    11401 gcaccacgcc gttcgcccgc atcctgatgg cctttgcgac accctctgaa gccggaccta
    11461 cgagacgccc tgcaggctct ccagctgcgc cagcagcgtg tcgcgctggg cgctgatctc
    11521 ggcgagccgc gcgcgctcct tggcaaccac ctcggccggg gccttggcgg tgaagccggg
    11581 gttgtcgagc ttggcggcca ggcgctcgac ttccttgccg agcttgtcgc cctcgcgagc
    11641 caggcgcccg cgctcggctg cgacatcgat caggcctgcc agcggtacca gcagttcgag
    11701 ttgtccgacg acctgcgccg ccgacggcgg agcttcctcg ccgtcgagcc aggtgctgcc
    11761 accgagtttc gccagggcgt gcaggaggtc gcggtgctcg gtcatgcggc ggcggtcctc
    11821 gtcgctgccg ttgcgcgcga gcagcggcag ctggcgcccc ggcgcgatgt tgagctcgcc
    11881 gcgtatgttg cgcaccgcca cgatcacctg cttcagccac tcgatgtcgg cgctcgcggc
    11941 ctcgtcgagc agcgacacct cggcctgcgg atacggcgcc agcatgatgg tcgcgcccgc
    12001 acgtcccgcg agcggcgcca cgcgctgcca gatctcctcg gtgatgaacg ggatgaacgg
    12061 gtgcgcgagc cgcagcaccg tctcgagcac gccgagcagc gtgcgccgcg tgccgcgtgc
    12121 ctggcgcggc tcggcgctgg cggcgagcag cggcttgctg agctccaggt accagtcgca
    12181 gtagtcgttc caggtgaact cgtagagcgc ctgcgaggcg aggtcgaatc ggaagcccgc
    12241 gatcgcgcgc tcgatctccg cgatggtctg ctgcaggcgc gagaggatcc atcgatcggg
    12301 cagcgccagc gtgaaatcgg cgtccggcgc cgtggcgcag tccttgccct cggtgttcat
    12361 catcacgaag cgcgcggcat tccacagctt gttgcagaag ttgcggtagc cctcgaggcg
    12421 gcccatgtcg aacttgatgt cgcgtcccgt ggaggccagc gcgcacagcg tgaagcgcag
    12481 cgcgtcggtg ccgtgggcgc ggatgccttc ggggtattcg cgccgcgtgg ccctggcgat
    12541 cttctcggcg agctgcggct gcatcatgcc cgcggtgcgc ttggcgacca gttcctcgat
    12601 gccgatgccg tcgatcaggt cgagcgggtc gatgacgttg cccttggatt tggacatctt
    12661 ctggccgtgc tcgtcgcgga tcagcccggt gacgtagacc ttgcggaacg gcacctcgtc
    12721 catgaacttc agggtcatca tcaccatgcg cgcgacccag aagaagatga tgtcgaagcc
    12781 ggtgaccagc acgtcggtgg ggtggaaggt gcgcagctcc ggcgtgtcgc gcggccagcc
    12841 cagcgtcgag aaggtccaca gcgccgagga gaaccaggtg tccagcacgt cctggtcctg
    12901 gcgcagcggc atatcgccga gatcgtgcac cgcgcgcact tcctcctcgc tgcgtccgac
    12961 atagacgttg ccggtggcgt cgtaccaggc ggggatctgg tggccccacc acagctggcg
    13021 cgagatgcac cagtcctgga tatcgcgcat ccacgcgaaa taggtgttct cccactgctt
    13081 cggcacgaac tcgatgcgcc cgtcctccac ggcgcggatc gccggttccg cgagcgcttt
    13141 ggtggccacg aaccactggt ccgtcaggta gggttcgatc accacgccgc tgcggtctcc
    13201 gcgcggcacc ttgagccggt gcttttcgat cttctccagc aggccgagcg cctcgagatc
    13261 cgccacgatg cgcgcgcgcg cggcgaagcg ctccaggccg cggtagggcg caggcgcgtt
    13321 ctcgttcagc tcggcgtcga tggtgaacag gttgatgcgc ggcagctcgt ggcgcttgcc
    13381 cacctcgtag tcgttgaagt cgtgggccgg cgtgatcttg acgcagccgg tgccgaaggc
    13441 cgggtcgacg tagtcgtcgc cgatgatcgg gatcagccga tccgtgagcg gcaaccgcac
    13501 cttgcagccg atcaggttgc ggtagcgctc gtcctcggga tgcacggcaa cggcggtatc
    13561 gccgagcatc gtttccgggc gcgtggtggc aaccaccagc tggccgctgc cgtcctcgag
    13621 cgggtagcgc aggtgccaca ggctgccgtc ttcttcctcc tgcaccacct cgaggtcgga
    13681 gatcgcggtg tgcagcttcg ggtcccagtt gaccaggcgc tggccgcggt agatcaggcc
    13741 ttccccgtgc aggcgcacga agacctcgcg cacggcctcg gagagctcgg ggtccatcgt
    13801 gaagcgctcg cgtgaccagt ccaccgagtt gccgaggcgg cgctgctggc gcgtgatggt
    13861 gccgccggaa tgctccttcc agtcccagac gcggcgcgtg aattcctcgc gccccagcgt
    13921 gaggcggtcg gtgccctcgg ccgcgagcag gcgctcgacc accatctgcg tggcgatgcc
    13981 ggcgtggtcg gtgccgccct gccacagcgt gttgtggccg cgcatgcggt gatagcggat
    14041 cagcgcgtcc atgatggtct gctggaacgc gtggcccatg tgcaggctgc ccgtgacgtt
    14101 gggcgggggg atcacaatgc aatagggggt ggcgccgccg cccgggcgga agtagccgcg
    14161 ctgctcccat tgcgcgtacc agcggccttc gatctcgtcc gggctgtagg tcttgtccat
    14221 gctctcgctt ccgtcgccgc ggccacctgc tcggcaggtg tgcatgcctg cggcgccggg
    14281 gccggcgcaa aaaatgcaaa attctatagt gccgcgatgg cgcgcagcaa ggaaaccgcg
    14341 ttcaccccgg cgttgcgggg ccggctttgt cggccagcag ttgccgcatc cgcgcttcca
    14401 gccgttcacg cagttgtgcc tcgagcagcg gcaggaactc gtccatcagg tcgtccacga
    14461 tggatcgggc ctccacttcc agtgccgcgc cgaggtcgat gccgggcgca aacgccgcgc
    14521 tcaccaggcc gggtgttgcc tgcgccgccc ggggcagctc gaccacgtcc tgcaggaccg
    14581 ggatgtcgcc gtaggtcggc gcgtcgtgcg cggacgggtc cagcgtcgcg ctcaacgact
    14641 gcagctcgtc gagcaggccc cggcgcgggt cgctgtgctc aggcgttgtc tcggtcaccg
    14701 gcgctccgca gatgttgcat gtcgtggtgc tggagctgat agccgcggtc cttgtagaag
    14761 ctccagcgct ggcggctggc ggcgagtgcg tgcggatcgt tgagcacgat ctcggcgacg
    14821 cgctcgaagc ggctgaacag cggcggcacc gcggcagaga gatttatcat cacgtcgtgg
    14881 tgatccgcgg cggcctcgcc cgagcccacc tccaccggtg cgggctcgcc ttcggcggca
    14941 ccgagcagcc ggtgcggcac gaagctggtg tcgcgaaacg accacagcag cgcgtcgatg
    15001 gccccggcct cggccgtgtc actggcatgc acgtagacct ggtgtcccga ctgccaggcc
    15061 ttttccgcca gccggcaggc gaagctcagc ttgccgtgcg gttcgctcac cggcagcagg
    15121 tagaagtcga tgcgggtcat gccgcggcgc gtctcaatcg gccgccttgt ggtgccgtgc
    15181 gcgatcgagc aggtagcgcg tgagcagcgc caccgggcgg ccggtcgcgc ccttcgccgc
    15241 gccctggtcc caggccgaac ccgcgatgtc caggtgcgcc cagcgctgct ttttcgtgaa
    15301 gcgcgacagg aagcacgccg cggtcacgct gccggcaccg gggccgccga tgttggccac
    15361 gtcggcgaaa ttgctcttca gctgctcctg gtagtcgtcc cacaggggca tctgccaggc
    15421 gcggtcatgg gtgtcgctac ctgccgcgag cagttcggcc gcaagctcgt cgttgttgct
    15481 gaacagcccg ctggcgtgct tgccgagggc gaccacgcag gcgccggtca gggtcgctat
    15541 gtcgatcagg gactgcggct cgaagcgctc ggcgtaggtg agcgcgtcgc acaggatcag
    15601 gcgaccctcg gcgtcggtgt tcagcacctc gatggtgatg ccggccatgc tcttcaccac
    15661 gtcgccgggc ttggtcgcgg tgccgctcgg caggttctcg caggccgcga cgatgccgac
    15721 cacgttgagc cgtggcttca gctcggcgat ggccctcatg gtgccgagca cgctggccgc
    15781 gccgcacatg tcgaacttca tctcgtccat ctcggcgccg ggcttgatgc tgatgccgcc
    15841 ggaatcgaag gtgatgccct tgccaaccag cacgtgcgga cgcgcacccc tggcgccgcc
    15901 gcggtattcc agcacgatca gcttcgccgc ttccttgctg ccggcggtga cggagagcag
    15961 ggcgcccatc ttcagctccg ccatcttctt ctcgtccagc accttgcagt gcaactgcgc
    16021 gtgctgcgcg gccagcgcgc gcgcctgtga cgccaggtag gacggcgtgc agatgttcgc
    16081 tggcaggttg cccagctcgc gcgccaggtt ggcgcccgcg gccatggcgc gcccctcggc
    16141 gagcgcgcgc acgctggcgg cggaaggccg ggtcgcgctg ccaagcacga ctttcttcag
    16201 tcgtgagccg ggttcgggct gcgacagggt gtgcgtgtag cggtagccgg ccgcgcccgc
    16261 gagcaggccg agctgttgtg cctgccagtc gcggtcgcgc tcattcgtca cgacgtcatc
    16321 gatcagcatg acgaggtcgg tggcgccggt ggcctgtacc gcgcccagcg cgtgcatgac
    16381 catcttgcgg aaggcgcccg cgtcggcggc ctcgggcttg ccggcgccga gcaacagcag
    16441 gcgttgcgcc gcgataccgg caggatggtg cagcagcacg ctcttgcccg cggcgccgct
    16501 gaagtgctcg gtcttcacga gccgcgcgag ctgcccgccc gtggcctggt cgaggcgcgc
    16561 ggccatggtg ccgaatttgc cgccgtcggc gaccggcagg accaggcagc cgcagcgttc
    16621 ggcggccgga tcggcgatgt gcttgatgct gatgtccatc tctgctcctg taggcatggg
    16681 gcgtcggcgg cggatgccgt gcgcagccct cgaaaaggtg caaattatat acgcggctcg
    16741 cggcgaggca ctcagccgcg cttcggcagc aataccacgc gcgtgccgga gagccggtcg
    16801 tgccaggtgc gcttctcgcg gtcgaacagt gcccaccagt agccggcgcc gaggcacagt
    16861 gccgacagcg ccgcgccgcc catgcggatc atggcctggg gccagctgat cggactgccg
    16921 tcgggctgct gcaggcgcaa ccgccaggcc tgcatgccca gtgtttgccc ggatcgggtc
    16981 cagaaccagc cgaagaacaa tgccgtcgtc gcggcgatcg cggcctgcag cagcagtccg
    17041 gatacggccg ccttgccgct ggcgcgtatg gcggcctcgc ccacgctggc cgcgcgcagc
    17101 cccaccgcga ccgccgtcac ggtccagccc accgcaaaca gcagcaggct gtcgtagagc
    17161 atcgcggcga agcggcgcag cagtccgggg gccggcaggg cgtccggcga gccggcggga
    17221 gatcgtgtca tgctagtatc gtgcaaggtc gggatcgtgt gcctgatgcg cctcgcgcag
    17281 gtgcgcagcg gccggacaca cgttaccaaa aggtaagggc cgcgcaaacc gccgttcacc
    17341 gttcgggctg cacaatgccc ggcaaatcca gccggggcgg gtgcagtgca gacagcggaa
    17401 gggatgcgtc gcgtccatgg agcggaattg atgcgcattc tggtgatcga agacgatgcc
    17461 agcgtcctgg actacatcag ccgcgggctg cgcgagagcg ggttcaccgt ggaccaggcc
    17521 gccgatggca aggacgggct gttccgcgcg agcgcggaga aatacgacgc gctggtcgtc
    17581 gatcgtatgc tgccgggcgt ggacggtctt gccatcatcc gcacgctgcg cggggccgac
    17641 aacaacacgc cggcgctgat cctgagcgcg ctcggcgagg tcgacgaccg cgtgaagggc
    17701 ctgaaggccg gcggtgacga ctacctcgtg aagccgttcg ccttcgccga gctgctggct
    17761 cgcatcgaag cgctgctgcg gcgcaaggac tcctcctcgg ccgtgacccg gctgcgcgtc
    17821 gcggacctgg agatggacct gctgtcgcac aaggtcacgc gcgccggcaa gtcgctgaac
    17881 ctgcagccgc gcgagttccg cctgctcgag tacctgatga agcacgccgg ccaggtggtc
    17941 acgcgcacca tgctgctcga gaacgtctgg gactaccatt tcgacccgca aaccaacgtc
    18001 atcgacgtgc acatcagccg gctgcgccag aagctggaca aggaattcga caagccgctg
    18061 ctgaagaccg tgcgcggtgc gggatacatg ctggatgaac ccgaacagga ttttccgggc
    18121 tagcctgttc cgctacacgc tgaattacct ggtcggtgtc agcgtggcgg tcttcgtggt
    18181 gctggccgtc ggctacgcct cgctcaccct cagctatttc cgcgacatga acgcgctgat
    18241 cggtgcggag acgcgcgagc tcgaggcgcg gttcgcgacc gagggcgcgc gcggtgtatc
    18301 cgtcgtcatt gccgatcgca tccaggcctc gggctttccg cgcctgttct acctgctggt
    18361 cgacgccgag gggcagaagg tcgcgggcaa cctgtcgcgc tggccggagc gcttggggca
    18421 gggcgaggaa tggctgggtt tcgaccgtac gcttgcggaa atcagcgggg gggagcagga
    18481 gttcgatttc gtcggtgcca gcacgcgctt tgccgacggc tcgcggctgc tggtcgcgcg
    18541 gcactacaag gacatcagcg agttcctgcg catcacgctg accgtgctgg tgcagggcat
    18601 gctcgccacc atcgtgctgg gcgccgtctg ggcggccatc ctcgcgctga tcctcgagcg
    18661 gcgcatcggc gcgatcaacg acagcatcgc gggcatcctc tccggtgatc tcagccagcg
    18721 catcccggtg gccaggggtg gcgatgacga cgacttcaag cgcctgatcg tcaacctcaa
    18781 tcacatgctc gatcgcatcg cgcaactgat ggaggacctg aagcagctat cggacaacat
    18841 cgcgcacgat ctgcgcacgc cgctcacgcg cctgcgcaac cggctcgcca gcatcgagtt
    18901 cgacggacag ggcgggcgca acgaggcggt cgcggcactg ttgcaggagt ccgacgagct
    18961 gctcgcaacc ttcaacgcgc tgctgcgcat cgcgcagatc gagtcgggca accggcgctc
    19021 tggcttcgcc gccaccgacc tggtgaccat attgcgcgac gtctgcgagt tctatgagcc
    19081 gctggccgcg gacaaggagc agcgggtgag cgtggtgctg ccgccgagtt gccccggcgt
    19141 gttcgaccgc gacctgctgt tccaggcctt cgccaacctg ctcgacaacg cgatcaagta
    19201 cgcgcccgcg ggcggtcata tcgaggtgcg gctggatgcc ggcacggacg tcgcgacgat
    19261 cagcgtcgcc gacgacggcc ccggcgtccc cgccgcggag cgcgacaagg tgttccagcg
    19321 cttctaccgg gtcgaggcga gccgcagcgg tcatccgggc aacgggctgg ggctcagcct
    19381 ggtgcaggcc gtgatcaacc tgcacggcgg tggcgtgcag ctcggcgaca acgcaccggg
    19441 cctgcgtgtc agcgtgtggc tgccgcgcgc cgctgcgcct gcctgaggcg ctcagcccgg
    19501 tggtgccagt tcgagcgcgc tggtgtcggg cgtcacggtg cgccgttcgg ggcgcaactc
    19561 ctcgagcggc gcgcccggcg gtgcgagcga gatgtgactg atgtccggta ccgcaaccgc
    19621 gagcgccgcg ccttgcgggc cgagcaccgc gcccggttcc gcgagcgtga tggcgctggt
    19681 gtccggtggc ggcgccggcg ctgcggcctc gcgctgcgcc agcaggtcgg cgccgacggc
    19741 ggcaatgctg atgtgggagg tgtcgggcac gaccggcagg gggcgttcct cctccggggt
    19801 cgttgcgaag gcgcttgcga gccggatgtg gctggtgtcc acctcgatgg cggtagcggg
    19861 gcgccgttcg tgcgcggcca gcaactcgcc gccgggtggc gcgaggtcga gtcccgcggc
    19921 ccgggtggct gcggcggaat ccggtgccag cgggcgcagc gtcgcgcgcg caccggcgcg
    19981 ttccatcgcg ctgcggtact tcgcggcagt cgcggcatcg acgttgcgct tgagcgtatg
    20041 catgccgccg tcgagcagcc gcgccacggt ttcgggcgag gtcttgaaca gtcgcgccag
    20101 gttgtcggcc accgtcgccg gcgcgaagcc ggtgagcaac tcgccaccga agactatgtc
    20161 gaaacgctcg tcactcatgc cgctccgcct cggggtcctt gctccatcgc tgcgccgaag
    20221 tatatgccac gaggcgacac cgcgggccgt gctttggcat aatgcgcggc ctccatgcgc
    20281 cagcccgtga tccagtgccc gataccgtga cccaggacgt gctccccaac gagttggtgc
    20341 cgacccagtt cgccgcctcg ctggcgcgca tgctgcgcga gcgcggactg gatccggcgc
    20401 agtccacgct ggccgccggc atcggcttcg acgtgctgga cccgcaggcg gcggcctacc
    20461 gcagcgagat tcccgcgctt gcctacagcc ggctctaccg tagcgtgatg accgcattgc
    20521 aggacgagac cttcggcctg cggcgcgatg tcgggatgcg gcccggcgcc ttccgcatga
    20581 tgtgctactg cctgctcggc tgcgcgaacc tgggtcgcgc cattgcgcgc atctgcgagt
    20641 tccaccaggt gttctacgac ggccaactcg agatgcgcct gaagcgcgag ggtgaatcgg
    20701 cgcgcttcga gatcgtcgtg ccgccgcagg cgaatgcgct cgccgctgca ggcgataccg
    20761 ccttcctgat gtcgccggcg gccgcggcct gcggcctgtc gatgtggcac cgcttcttcg
    20821 gctggctcgt cggtcacccg ctgccgctcg atcgcgtcga gctgagcggt tcgccgccgg
    20881 cgaatctcgc gcgctaccgc cagctgttca actgcgagct gcagttcggc gtcgtgatcc
    20941 ccgcgttcgc ctttccggcc gattacctgg cgtatccgct ggtgcacaac gaggactcgc
    21001 tgcgcgattt cctgcgcacc gcgccctacc agctgctgac catgccggcc gatcccggca
    21061 aacagagcct ggtggaccag gtgcgcgcgc tgatcgggca cgatttcagt cgcggtttcc
    21121 cgagtttcga gcgcatcacc gagctgctgc acatgtccgc gcccacgctg cgccggcgtc
    21181 tcaagcgcga gggcaccacg ttccaggagt tgaaggacag ctgccgtcgc gaggcctcga
    21241 tcgattacct gaaccgcccg gagctgtcga tcaccgacgt ggcgatcctg atgggcttca
    21301 cggatccgag cgcgttctac cgctcgttca agaaatggac cggcatgaca ccgggcgagc
    21361 accgcgcgct cgaggcacgc tgagcgcggc ctgactgcaa ttgctatttc ctgttgaccc
    21421 gaattgccat tgaggccgcg cggcgcgcgg gtatccttgt cgccctgaaa tctgcgcgcc
    21481 gcggcagtcc gcaagggccg gccccgggag cggcgcgcgc aaccaccggc ggagttccac
    21541 atggctcacg aggcattgat tttcgacgcg atccgcaccc ctcgcggcag gggcaagccc
    21601 ggaggcagcc tctacgaggt caagccgatc gacctgctcg tcaacctcat ggaggcgatg
    21661 aagcagcgca acgatctaga cacctcgcgg gtagaggacc tggtgatcgc ctgtggcgaa
    21721 cccgtgggcg agcagggcca ggatatcgcg cgcaccgcgg tgacctactc cagctggggc
    21781 gaggccacgg cgggcgtgca gatccaccgc ttctgcgccg gcgggctgga tgccgtgaac
    21841 atggccgcgg ccaaggtggc ggccggcttc gaggacctcg tggccgccgg cggcgtggag
    21901 tcgatgtcgc gcctcggcat cggcgccagc ggcggggcgc agggcgatcc ctgggtcgcg
    21961 atgcgcacgc tgttcgtctc gcagggcatc ggtgccgacc tggtcgcgac cctgaacggc
    22021 ttcagtcgcg aggacgtcga ccgctacgcg ctgatgtcgc agcagcgcgc ggccgcggcg
    22081 cgtgccgagg ggcgctttcg ctcgatcgtg ccggtgcgtg acatgaacgg ggtgacgatc
    22141 ctggatcatg acgagttcat ccgcccaggt acgaccctgg cggatctcgc gcggctcaag
    22201 ccctcgtttg cgcagttcgg cgagtggggc caggacgagc tgttgaagct gcgttacccg
    22261 caactcgaat ccgtcgagca cgtgcatacc cccggcaatt cctcgggcat cgtcgacggc
    22321 gcgggcctcg tgctgatcgg caacgagcgc gccgggcgcg agctcggact gaagcctcgc
    22381 gcgcgcatcg tcgcgactgc gctggtcggc accgagccga cgatcatgct cgagggcccg
    22441 gctcccgcgg cacgcaaggc gctggccaag gcagggctca aggtggagga catcgacctg
    22501 ttcgagctga acgaggcctt cgcggccgtg gtgctgcgct tccaggcgct gatgggtgtg
    22561 ccggacgagc gcatcaacgt caacggcggg gcaatcgcga tggggcaccc gatcggcgcc
    22621 accggcgcca tgatcctggg cacgctgctc gacgagctcg aacggcgcaa cctcgagcgc
    22681 ggcctctgca cgctgtgcgc gggcggcggc atcggcatcg caaccatcat cgagcgcgtg
    22741 tgatccgcgc gccgagcgcg gatccggtac agcggggagc agggaccatg ggatacatca
    22801 ctctgaacaa gggcgcggaa ggcatcgtcg agctggtgtt cgaccagccg ggctccagcg
    22861 tcaacacgat gggcctcgag tacgacgccg cgatgaaggc ggcggtcgcc gagctgcaag
    22921 gcatggttgc agcgggtggc gtcaggggcg tctatgtgcg ctccggcaag ccggggcagt
    22981 tcttcgccgg cggcgacatc aaggaaatgc tcgagatgga tctcgacgtg ccggccgagc
    23041 gcaagcagca gatgttcgag ggcacgctgg cgaccaaggc gccgttgcgc acgctggaga
    23101 cgctgggcgt gccggtcgcg gtcggcatca atggtgcagc gctaggcggc ggcttcgaga
    23161 ttgcgctggc ctgccaccac cgcatcgcgc tggcgggcgt ggacctcggc ctgcccgagg
    23221 cgcagatcgg gctgatgccc ggtgccggcg gcgtggtgcg cctgacgcgc ctggtgggta
    23281 tgcaggaagc gatcacgctg atcagccagg gcacgcgcct gaaggccgag aaggcgctcg
    23341 aaaagggcct gctgcacgag atcgcggcga gcgaggacga gatgcgcgcg cgcgcccacg
    23401 cctggctgct ggccaacccc ggggcgaagc agccgtggga tagcgaaggc tacaagattc
    23461 ccggcggtgg gccggaccac aaggccaacc agggcctgat gatgctgggc ccggtcaacg
    23521 tcatgaacca gaccagggga ttgatgccgg cgcagaaggc gattttcgcg gcggttgccg
    23581 attgcgcgcg cgtggatttc gacaccgcgc agaagatcga ggcgcgctat ttcctcagcc
    23641 tgctgctcga ccagaccgcg cgcaacatga tgacggcgtt cttcgtgcag atgaacgcga
    23701 tcggcaaggg cgcgagccgc cccgcgggca tcgcgcggcg caaggtgaag accctgggca
    23761 tcctcggcgc gggacagatg ggtgcgggca tcgcctacgc tgcggcgcgc aagggtatcg
    23821 cggtgttcct gaaggacatg gatctcgcgg cagccgagcg cggcaaggac tatgcgcgcc
    23881 aggcgtgcgc gaagaacaag cgcatgagcg acgccgagcg cgaggcgctg ctcgcgatga
    23941 tcacgccgac cgcggactac acggatctcg ccccctgcga cgcggtgatc gaggcggtgt
    24001 tcgagcgtcg cgacgtaaag gcagcggtgg tgcgcgaact cgaggccgtg ctcgggcaca
    24061 ctgcgctgat cgcctcgaac acctcggcgc tgccgatcac cgagcttgcg gaggcctcgg
    24121 tgcgcccggc gaatttcatc ggcatgcact tcttctcgcc ggccgacagg atgccgctcg
    24181 tggagatcat ctgtggcgag cagaccggcg acgaggcgct cgcggcggcc ttcgacctcg
    24241 cgcagcagct cggcaagacg ccgatcgtgg tcaacgacgc gcccggcttc ttcaccacgc
    24301 gcgtgatcgg caagaccatc tcgcagggcg cgcagatggt gctggagggc atcgacccgg
    24361 tgctcatcga ggcggcggcg cgcgccaacg gctcgccggt gggcccgctc gcggcgatcg
    24421 acgagatcag ccaggaaacg gcctaccgca acggtctgca gatgaaggcc gacgccgagg
    24481 cccgcggcga gacctggcag gagcagccgg ccggtgagct ggtgcggcgc atggtcgagg
    24541 agttcggccg caaggggcgg cgcagcggcg gcggctacta cgagtacccg gaaggcggca
    24601 agaagcacat ctggccaggc ctcaagcagc actttgcccc aaatgggtgc aaagaagtgc
    24661 cgttcgagga catcaaggac cggctcgtgt tcgcgcagtg cgtcgaagca gtgcgtgcaa
    24721 tgcaggaagg cgtgatcacg agcgttgccg atggcaacat cggctcgatc atgggtatcg
    24781 gcttcccgcc gcataccggc ggcgtgttcc agtgcatcaa cgcctggggg ctcaagcgct
    24841 tcgtggcgcg ggcgcgcgag ctggcgcagg cttacggcgc ggatttcgaa ccgccgcggc
    24901 tgctgctgga gcgcgcggag acgggcgaat tgttcggctg atccagatca tgccacgcaa
    24961 gggtggcgca cgccgcggct gttcatttgg gatcgccggc ctataatgcg gatgagtcaa
    25021 ttttccggca gcggtgcatg gtcaggaaga ttccggacgg caaggcgggc ggcgctgaca
    25081 gcatgcgccc gtcgcgcacg gacaccgaat ccatattcgg tgacaagcgc agcgggtggg
    25141 atcggcgcaa gcagcaggtc tccgtgaccg aaagccggcg tgaggtgagc gatcgccgca
    25201 agaccgggcg cggtgcccag ggggcatggt ggctcgagcg cgattacgtc gagagccacc
    25261 atttcgtgca gaaatcctcc agctcccgga cccgcaagcg cgacgacacc ccatccgggg
    25321 agtgagagcc ctccgatcct gatatgatgc gcgccgcttg tgcggaggta gtttcatgca
    25381 gcccgatacc aggagcccgt ctgcgcgcag ctgtgtcgcg ctgctcgtct tgctgttgtg
    25441 ctgtgccctg ttcgcgcgtg cacgagccgg cgatgaagat ggctttcgcc cctacgatcg
    25501 cgaggccgat cccagggcgc agatcgccgc cctgctcgag gaccgctcca gcggcaagcg
    25561 catcctgctg accttcgggg ccaactggtg ttcggattcg cgcagcctcg cgcgacgcta
    25621 ccgcacgccg gagctggccg cggtgctcga gcgcgagttc cgcacgctgc acgtggacgt
    25681 cggcaggagc cagcgcaatc gcgatatcgt ggagcaatac ggtaatccga ccgacatggg
    25741 cattccctcc gtggttctgc tcgcgcctga cgggacgttg ctgcacaccg accacggcag
    25801 cctgtccgcg gcaggtcgca tgagtgatcc ggaattgatc gcattcttcg agcgtctcgc
    25861 ggcccggtac ccggtgccgt gagccatgat ttccccactg caacccacac gaggatgacg
    25921 gagcagcaca tggccgaatt gatggtcgag atcacgcgcg acaacgcgca gcgcatgctg
    25981 atcgaggagt cgcacgcgcg ccccgtgctg atcgatttct gggccgactg gtgcgcgccg
    26041 tgcaaggcgc tgatgccgat cctcgacaag ctggtgcgcg aatatgccgg ccaggtgatg
    26101 ctcgcgaagg tcaactgcga cgagctgcag cctatcgcgc agcagttcgg ggtgcgcagc
    26161 ctgccgaccg tgatcctgat gaaggacggc cagccggtcg acggcttcgt gggcgcgcag
    26221 cccgaatccg cggtgcgcca gatgctcgag aagcacctgc ccaagccgtg ggacatcctg
    26281 ctgcaccagg cgctggaact gctcgaggcg ggcgatgcgg ccggggcgct cgcgccggcg
    26341 cgcagtgcct acgaggagtc gcgccagcgt gccgacatcg ccaaggtcta cgccgacatc
    26401 ctgatcgagt tgaaccgcgt ggacgaggcg caggtggtgc tcgattcgat cccgatggcc
    26461 gaccaggacg gtgacttcga acgcctgctg tccgagctgc agctgaagaa gcaggcggcc
    26521 gacacgcccg agatccaggc gctgctgcag gcgcgcgcgc gcgatcccga caacatggac
    26581 accggctacc agctcgcggt gcagttcagc caggcgggcc gttcgcgcga ggcgctggag
    26641 ctgctgatcg gcatcctggg ccgtgaccgc gagttccgtg gcggcgaggc gcgcaagacg
    26701 ctcctcgaca tcatccgcgc gctcggcaag ggcgacccgc tcgcggcgga gtaccagcgc
    26761 aagctctttg cgctgatgta ctgaggagta ggtctggccg ttgcctggaa attgctcggc
    26821 tgttttcacg cccgcgcggc tgccgtaatg ctcgagagcc gcacgcgcgg cgcgtcgctg
    26881 gctgcgctgc ccgcccttgc cgtgctgggg acgccgtgaa tacgtccctg taggctcgag
    26941 cgccgcatcc atgcggcgca cgccccctca cggcaagggc ggccagctca gcccgctcgg
    27001 tcgaagcggt gcctcctttg gctattcctt cgatccggct cattccgatg ttccacagcg
    27061 ccgagtggac tcggccacgc atttgccccg aaggcattct gttgcgttgt ttctgcaggt
    27121 actcaaacat gtagcgatac ttggcgtatc aagctgcggc agtgcgtcag ccgagcggcc
    27181 tcgttgtccg gtcgcggcat aaggggccgt gcgccgcagg gatgcggcgc cagagcctac
    27241 agggacgtac ttgccgcggg ccccgttgcg gcggccgtac agcgaggcca gcgacggagc
    27301 aagtcaaagg ttcgaaatgg tccgaaattt cgcggttctc tccgcgcccg cgcccgcgga
    27361 gatcccttcg tcagatgtac cgcgccacca tcgcgtagcc ggcatcgcgc agctcgcgtg
    27421 cggcttcggg gagcgggaac ttcaccacgt gaccggagcc cggtgccacc tcgcggcgct
    27481 cgccggcttc gctggtcatg tcggtgaggg tgaattcgca gttgccttgc ggggtcatca
    27541 gttccaggcg gtcgcccagc gcgaagcggt tgttgaccga gacggtgagc atggcggagg
    27601 cggcgtcgag gtcgagcacc tcgccgacga actgctggta atcgcccttc gagacgccgc
    27661 gctcgtagtt ctggaactcg cccggcggat ggcggcggta gaagccctcg gtgtagccgc
    27721 ggctggcgag cttctcgagt tcctccatca gccccatgtc gaactcgcgg cccgcgagcg
    27781 cgtcgtcgat cgcgcgacgg taggcctgcg cggtgcgcgc cacgtagtag tgcgacttgg
    27841 tgcggccctc gatcttcagg cagtcgaccc cgatgtcgat cagctggcgc acgtgctgga
    27901 tcgcgcgcag atccttcgag ttcatgatgt aggtgccgtg ctcgtcctcg taggcgggca
    27961 tgtattcgcc ggggcggcct tcctcctgca gcaggtagat gcggtcctgc acgggcgcag
    28021 gctcgggttc gggcgcctgt agcggcaccg gttcgtggat cgcgaccacg tcgcccgatt
    28081 cggtctgcac cgcctcgtgc gcctggtact tccagcggca ggtgttggtg caggcgccct
    28141 ggttcgagtc acggtgcgtg atgtagccag acagcaggca gcgaccggaa taggcaatgc
    28201 agagcgcgcc gtgcacgaag acctcgagct cgatgtcggg gcattcctgg cggatctcgg
    28261 cgacttcctc cagcgaaagc tcgcgcgaga ggatgatgcg cgtcagcccc atgcgcttcc
    28321 agaacttcac cgtcgcccag ttcaccgcgt tggcctgtac cgagaggtgc accgggatct
    28381 cgggccagcg ttcgcgcacc atcatgatga gccccgggtc ggccatgatc agcgcgtcgg
    28441 gtttcatggc gatcaccggt tccatgtcgc gcaggtagct cttgagcttg tcgttgtgcg
    28501 gcgagacgtt gctggcgatg aagaactgct tgcccagccg gtgcgcgtag tccacgccct
    28561 cggcgaggcg ctcgatgcgg ctgaagtcgt tgttgcgcac gcgcaggctg tagcgcggct
    28621 gcccggcgta gaccgcgtcc gcgccatagg cgaaggcata ctgcatggac ttgtaggtgc
    28681 cggccggaga cagcaattcg ggcttgcgca tggactggag cctgcatcgc aataaggggg
    28741 gagggcgcgt attatccggg atcggcccgg ggcgggccag tgaaatacgg acagtccgct
    28801 agggaggcaa ggtacggatg cgcatgcagc agaccatcga acgcaagctc gccgaggctt
    28861 tcgcgccggg ggagctggag gtgctcaacg agagccacca gcacagcgtg ccgccgggct
    28921 cggaaacgca tttcaaggtc gtgctggtca gcgcggcctt cgagggcaag cgcagcgtcg
    28981 cgcgccacca gcaggtctac aaggtgctgg ccgaagaact cgcccgtggc gtgcacgcgc
    29041 tcgccctgca cacctacacg cccgcggagt gggcgcaacg cgccgcgcac gcgcctgatt
    29101 cacccgcctg catggggggc ggcaaacacg atcactgagc cttcgccgga ccgtcctctc
    29161 ccgcttcggg ctgctccagt gccagcgtct cgcggcgctg gtcgtcggcg tagtagcgcg
    29221 ccagaacttc ctgcgagcga agccggcgct tgtgcccgcg atcacggtaa ccgttgtcct
    29281 ggtccgcgct gagcacgcgc gccttcacgt tgtcggccca ttgcgtctcg agcaggcgct
    29341 gcagccggcg ctgcgcgagc ttgtcgtaga ccggcgcggt gacttcgacg cggtggtcga
    29401 ggttgcgcgt catcaggtcg gccgaggaga tgaagtagcg cggttcaccg ccgttgccga
    29461 acacgtagac gcggctgtgc tcgaggaagc gatcgacgat gctgatgatc tcgatgttgt
    29521 cgctcacgcc ggcgacaccg gccagcagcg agcacatgcc gcgcacgatc acgcggatcc
    29581 tcacgccggc ctggctggcc tcgtagagcc ggtcgatcag ttcctcgtcg accaggttgt
    29641 tgcacttgag gaagatcgtg gccggcttgc ccgcgcgcgc gttgagcacc tcggcgtgga
    29701 taaggcgcag gaaggtggag cggttggtca gcggcgagac cgcgaggtgg cggaacttgt
    29761 ggcggcgata ggtgtggcgg atgaagtcga agacgtggtg cacgtcctcg gcgatctcct
    29821 ggttcgcggt gaacagcgcg agatcggtgt agaagcgcgc ggtcttctcg ttgaagttgc
    29881 cggtcccgat gtgcacgtag cggcgcagtt gcgtcccctc ctggcggctg atcaggatca
    29941 gcttggaatg cactttcagg cccggtatcc cgaagaccac gtgcacgccg gcctcggtga
    30001 ggcgcttggc ccagtcgatg ttggcggcct cgtcgaagcg cgcgcgcaac tcgatgatcg
    30061 cgaacacctc cttgccgttg agcgccgcgc agatcagcga attgccgata tgcgagttgc
    30121 gcgccacgcg gtagagcgtg acctggatcg cgcgtaccgc cgggtctatc gccgccgcgc
    30181 tgatcagctg ctcggtatag cggaacgagt ggtaggggta gttcagcagt acgtcgcgct
    30241 cggcgatcgc ggcgaagatg ttcgcgtgcc ggtcgatgcg aggcaccatc agcgggccga
    30301 tcggccggtg ctctagcgag cgcgcgccga gattggggaa ttccatgaaa tccttggaat
    30361 tgtgataacg cgcgccgggc agcacgttgt cgtagctgcc gaggcgaaag cgtttcacca
    30421 tgatgtcgag catgtcgttg ggcatgcgcc ggtcatagac caggcgtacc gggtcgccga
    30481 gcttgcgctt cttcaggctg ctcgagagcg cgtcgaccag gctctgcgtg atcccctcgc
    30541 cgagctcgag ctcggcatcg cgcgtgatct tgaacgtgaa tgcctcggcg cgctcgatgt
    30601 cgaaaacgcc ctggaacacc tgtggcaggc aggcgcggat gatattgtcg agaaccacga
    30661 tcacctggcg gctcgactgc gccgccggcg agggcaccac gatgaagcgc ggcagtcgat
    30721 ccgaggggat gttcacgatg gcgtagcgta tcgactggtt cctcagctgg atgcgcaccg
    30781 cgaagtagat ggagccatcc tcgagctgcg gagtggcggt ggcatccgag atgatgatgg
    30841 gcgcgagctc gggcatcacg cgggcgaaga agtactggcg cgcgaactcc cgctgctgcg
    30901 catcgagctg gcgctcgtcg acgaggtaga tgttgttctc gcgcagctgc gacaggcagg
    30961 tgcggtagac gcggtcgaag cgcatctgca ggtcgccgac cgcgtcgcgg atgtcgtcga
    31021 gcagtttttc ccagcgcgcc ttctcgcggc ccttcgcaaa ggccgccagc cggcgcacct
    31081 ccgcgtagcg cacgcggtag aattcatcga ggttgttcga gaagatgccc atgaagcgca
    31141 cgcgctcgat cagcggcact tcgggatcct cggcttcctg cagcacgcgc gcgttgaacg
    31201 agagccagct cagctccttg gggtaaagcg gcagggtctt gtggcgggtc ggcatgcagg
    31261 gatccgtgac cggtgggtcg gtgattgaaa atgcgcgggc agcgattaaa ctgccggccc
    31321 atcgcggcgg gcacagcgtg acttcttaat gcgcccggat gacattttta ttacagcgcg
    31381 cgccgcgatg ccacggaaat accgcacatg aatgaatctt ccggtcccgt ctgcgccgtc
    31441 ctcgacctgg gctccaacag cttccatctg ctgatcgggc ggctggcggg cgggcgcctc
    31501 gtgacgctcg accggcgcaa ggacatggtg cgcctcgccc aggggctgga gggcgacggc
    31561 cagctgagcg agccggtcat gtcccgcgcg ctcgacagcc tggcggtgtt ctcgcagcag
    31621 ctgcgcaata tcccgcgcgc caacgtgcgc gtggtcggga ccaacacgct gcgcgcggct
    31681 cacaacgccg acgtgttcct ggagcgcgcc gagaagatca tcggggtgcc gatcgacgtg
    31741 atcagcggcc aggaggaggg acggctgatc ttcctcgggg tggccaaggg gcacgcgagc
    31801 aacgcgatcc gccgcctggt catcgacatc ggcggcggct cgaccgagtt catcgtcggc
    31861 cgacgcaagg ccaagcgcgt cgagagcctg ttcatcggct gcgtgtcgat gagcgagcga
    31921 ttctttcccg agcgccgctt cacgcgcgag ggctaccagc gcgcgctgac gctggcgcgc
    31981 gcggagatcc agcatctggc cgacggttac ggcaccgcgc gctgggacga ggcggtgggc
    32041 acctcgggca cgatccgcta cgtcgaggcc gtgatcgacc gtctgacgcc ctgcgatcac
    32101 ctgatcacgc gcgagggtat cgagcgcgtc gtcgatcacc tgaaacgcag caagaagccc
    32161 gacgcgctgc aatcgctggg gctcagcccg gagcgcgtgc cggtgttccc gggcgggctg
    32221 gcgatactgc acgcggcctt catcgagctc ggcatcgagc gcatgcacgt ctcggactac
    32281 gcgctgaagg agggcgtgct ctacgagctg gccgaccggg gcgagcacga ggacacgcgc
    32341 cgccagacca tggattacct gaccaagcag ttcaacatcg accgccgcca ggccgcgcgc
    32401 gtcgagcggc tcgccaggtc gctgctgccg caggtcgcgc cgcagctgac cgtggagcgc
    32461 gccttcgcgc agcaggtgct ggttgcggcg gcgcgtttgc acgagctcgg cctcgcggtc
    32521 tcgcacagcg gctaccacaa gcacggcgcc tacgtgctgg agcatgccga catgcccggg
    32581 ttctcgcggc gcgagcagat gatgctgagc ttcctggtgc tcaaccaccg ccgcaaactg
    32641 cgtgcgcccg aggtgagcag ctacagtttc aggcccgact gggcgctggt gctggtgctg
    32701 cggctcgcga cgctgttcaa ccgcatgcgt atcgaccagc gcatgccctc gatacgcctc
    32761 gcgccgatgc gcaagggctg gagcatcgag ctcccgtcgg gctggctgcg caagcacccg
    32821 ctggtggagg aggatctgcg cgcggagaac gagttcctcg cggcgatcgg ctcggggctg
    32881 caagtgaaga cgcggcgttg aaggtcctgc cgtgagcttc accgccgcgc tgccggacat
    32941 cgaggccgcg cgccctcgcg cgggccgccc ggccgggcag ggcctgatcc gccagcagac
    33001 gaccgacttc acggtcgagg agctgatcga gatggacctc gacgagggcg gcgagcacct
    33061 gtggctgcgc gtgcgcaaga ccggcgagaa caccgagcac gtcgcgcgcc tgctggcgca
    33121 ggccgccggc atacgcccgc agcaggtggg ttacgcgggg cgcaaggacc gccacgccgt
    33181 gaccgagcag tggttcagcc tgcacctgcc ggggcggccg gatccggcgg acttcgcgct
    33241 gccgccctcg gtggagatcc tcgggatgcg ccggcgccgg cgcaagctcg ccgtcggcgg
    33301 cctgcgcggc aaccgcttcc gcctgctggt gcgcgagttt gccggcgatg ccgcgctgct
    33361 cgacgagcgc ctgcgcgaga cggccgtgcg cggggcgccg aactacttcg gcgcgcagcg
    33421 cttcggccgc ggcggcggca acgtggcgca ggcgctggcg tggatgcgcg gtgagcggcg
    33481 cgtcgatgat cgcaagctgc gcgggctgct gctctcggcg gcgcgctcgc agctgttcaa
    33541 cgcgctgctg gcgcgccggg ttcaggacgg ttcctggtgc gaggcacgcg cgggcgacgc
    33601 gctgatgctc gacgggcgcg gcagcttctt cgtcgccggg gagatcgacg caacgctgcg
    33661 cgagcgggtg gcgcgcggcg agctgcaccc gagcggcgcg ttgtggggca ggggagaact
    33721 cgccacgcgc tctggcatcg ccgggctgga gcgcgcggtg gcgaccgaac acgcggagct
    33781 cgccaccggg ctggagcgcg cgggcctgga gcaggagcgg cgtgcgctgc gcgtgatgcc
    33841 gcgcgagctg acgtggggct ggatcgatga caccacgctg gcgatcgcct tccggcttcc
    33901 cgcgggctgc ttcgcgacca cggtgctcga cgatgtgctg gactgtcgcg acgcggcgca
    33961 agcggccgtg gcgcgcccgg acggcgcggg agactgaacc gtcctgcctc agcttgcgcg
    34021 ctcggcgagc ggcagcactt cgagcgcttc gccacgggca gtgacggcca ggcagttcgc
    34081 gccgtcgagc gcttcgtcgc tcagcacggc gagcagttcg aaacgcgacg cgccgtccat
    34141 cgcgctctcc accacgctgc ccacgacgcg gtcggcgtcg cggtgccgca gttcgtcgcc
    34201 gaccgcgggg cacgccgggc cggcgccgcc gaggcgatgc aggcggcgtt tcacgctgcc
    34261 cttgtaatgg gtgcgcgcga tgatctcctg gccggtgtag cagcccttgc ggaaactcac
    34321 ggcgccgctg gtgtcgtagc cgagcatctg cggcaggaac tggtccacgg tggcgtgctg
    34381 gatctcggcc gcaccggcgc gcaccagtgc caggcgccaa cgctccgtgt tggcgaccgg
    34441 gaggcgcgag gccagacttt cccacagcgc tccggtctcg ctgcgcggcg cccagatctc
    34501 gaactgcgca ggctcgccgt cgcggcgcag caggcgccac gcgccggcgg cgccgagcgc
    34561 gcgcggctgc gcgggccaat ccgggaacag cgtgtgcaac tcggtctcca gtccggtccc
    34621 cagcagtccg atgcaagcca tgtcgcagcg tgccacgcgc acgcgcgaca gcgcggcgta
    34681 cctgccgagc acggtcgcgg tgtcgtcggc tatgtcggca cgcaggcgca gcagcacgga
    34741 gtcggggctg cgagcccaca gcaggaaact ggcgatgacg cgccccttcg gcgtgcagta
    34801 ggcgcccgga agcacgcccg cgggtgctgc atcctgcacg ttgcaggtca gttgtccctg
    34861 caggaacggc agggcctttg cgccctcgac ctgcaggaag gcatagtcgt cgaggcggat
    34921 ggcggcggtg ttcatggctt cgtggttccg gcatgcgctc ctgggcgctg gcggcgcagc
    34981 gcgggctgcg cgcgccggtg gctgacaggc ggcgaataat aacgcacact gcccgcgcta
    35041 cgagcggttg cggatacacg atggacgagg cggcggaaaa caggcgggtc tactggcaca
    35101 gccggcgggg catgctcgaa ctcgacctcg tgctgatgcc cttcgccgag caggtttatc
    35161 ccgggctcgg cgaggacatg cagcgtcgct atcgcgcact gctcgagtgc gaggatgccg
    35221 acctgttcgc gtggatcgtg catcgcggcg aaccggacga tcctgaactg aaagtcatca
    35281 ttgcgagggt gctgggcaat ggaatgcccc ggccagagct tccacctggc gccgtctagg
    35341 cgcctcctgt acgcgctgtg cgtgctgcac ctggcggccc tcgccgggct cgtcgctacc
    35401 gccctgccgt ccgctctcat gctggcgctg gccaccctgt tgctggcaag cctcgcgcgg
    35461 cactggcgcg cgcacggtgc gctggagcgc cgcggcggca cccggctcga gtttcgcggt
    35521 gaagcgtggt ggttgcacgg cgacggcgcg ccgcagccgg tgcggctgcg cgccgacagc
    35581 gcggtgctgc ctttcctggt cgtgctgcgc gtgcagcagg cgcggcgggt gcgctcgctg
    35641 atagtgctcg gggattcgct gccgcgcggg gagctgcggc gcctgcgggt gctgctgcgc
    35701 ctgcgcggcg cgcgctgaag cccgcgcccc gcttcagccg ggtgttagga cggagtcccc
    35761 ggcctcggcc gcgaggccgg ggtggtccag ggtgtagtgc aggccgcggc tctcgtggcg
    35821 cccgagcgcg ctgcggatga tcagttccgc caccagcgcg aggttgcgca gctccaccag
    35881 gtccgaggtg atgcggtaat tgctgtagta ctcggcgatc tcctgcttca gcaggtgggc
    35941 gcggctgagc gcgcgctgca ggcgcttggt ggtgcgcacg atgccgacgt agtcccacat
    36001 gaagcggcgc agctcgtccc agttgtggga gatcaccaca tcctcgtcgg agtcggtcac
    36061 ctgggaggcg tcccagtcgg gcaggccggc gctgatcgcg atgctctcgc cctgcgcgag
    36121 gatgtgctgc gcggcggcgc gggcgaacac gaagcactcg agcagcgaat tgctggccag
    36181 gcggttggcg ccgtgcagcc cggtgaacga tgtctcgccc acggcgtaga gccccgggag
    36241 gtcggtctgg ccggcgcggt cgatcatcac gccgccacag gtgtagtgcg cggccgggac
    36301 cacgggcagc ggtgcgcgcg ccatgtccag tccgagctcg aggcagcggc tgtagatcgt
    36361 ggggaagtgc gagcgcacga agtcctcgcc cttgtggctg atgtcgagat agacgcagtc
    36421 gataccgagg cgcttcatct cgtggtcgat cgcgcgcgcc acgatgtcgc gcggcgccag
    36481 ctcggcctgc ggatggaagc gcggcatgaa gcgctcgccg ttgggcagca gcagcacgcc
    36541 tccctcgccg cgcacggcct ccgagatcag gaaggatttc gcgctcgggt ggtacaggca
    36601 ggtcggatgg aactggttga attccatgtt cgcgacccgg cagcccgccc gccaggccat
    36661 cgcgatgccg tcgccggtcg cggtgtccag attggtggta tagaggtagg ccttgctggc
    36721 gccgccggtg gcgagcacca cgaagcgcgc actgaagagg tcgaccgagg ccgtgtcctt
    36781 gttcagcacg taggcgccgc ggcaacgccc ggcggtcgtg atcaggttga ccgcgatgtg
    36841 gcgctcgaac agctcgatgc tcgcatggcg ccgggcctgc tcgatcagcg tgtccgacac
    36901 cgcgcgcccg gtcgcgtcgg cggcgtggat gatgcgccgg tgactgtggc cgccctccat
    36961 cgtgaggtgg aactcgtttt cgccgcccgg cgtgagaagc ttcgtgaagc cgacgccgag
    37021 gtcgaccagc cactggatgc tctcgcggct gtgctccacg gtgaagcgca ccatgtcctc
    37081 gtggcacagg cctgctcccg cgcgcagggt gtcggcgacg tgcgagtcga tcgtgtcctc
    37141 gtcatcgagc acggcggcaa tgccgccctg ggcccaggat gtggagccgt cgccgagctc
    37201 gcccttgcag atcaccgcga cccgcgcccg gtcggcgacc tggatcgcga cgctcaggcc
    37261 ggcggccccg ctgccgatca cgagcacatc atgggtgtag cgttgcggca tccaggcggg
    37321 tccggggcgg ggtgaggccg aaatatagca caggcccctc cagtggccgg atcgcggcac
    37381 gattgccgct ttgtcacggg acccgccagc acgtatattg agcgccgcga acgtcacggc
    37441 ccctcgcgat gcccccgatc gcggggtcgc cgtggcgcgt ggctaacgga ctgaaaccag
    37501 ggtcgaacgg agtccttcga tgttttccgg ggaggagggt gatcagcaac tggtcgagcg
    37561 cgtgcagaag ggcgacaacc gggctttcga tgtgctggtg ctcaaatacc agcatcggat
    37621 ctattcgctg gtgacacgct tcatccggga tcccgacgag gtgcaggacg tcgtgcagga
    37681 ggcgttcatc aaggcttacc gcgccctgcc gggtttccgc ggcgagagcg ccttcttcac
    37741 ctggctctac cgcatcgcga tcaacaccgc gaagaactac ctggtgtcgc gcgcgcgccg
    37801 cccgcccggc gccgacgtcg atgtcgagga cgccgagcac ctcgagagcg gcgcggcatt
    37861 gcgcgacctg gcgggtccgg agaaccagct gatgaccgag cagttgcgcg cggtgatcga
    37921 cagggcgata cgcgcgctgc ccgaggatct gcgcacggcg ttgacgctgc gcgagttcga
    37981 ggggctcagc tacgaggaga tcgccgaggt gatgcgctgt ccggtgggca cggtgcgctc
    38041 gcggatcttc cgggcgcgcg aagccgtgga tcatgaaatc ctgcccctga tcgaggggaa
    38101 ctaatttgtc tgccgggtat ccgaagggtt gcacgtatcc gcttgggggt ggattttgag
    38161 ccagctgaat gcgcctttgc gcgagatgat ctccgccgtg gtcgacggcg aggccagcga
    38221 attcgaatac cggcgcgtgc tggagagcgt ggacgatgcc ggggtgacgg ctctgctggg
    38281 gcggcactac gcggtgcgcg cggtggcgcg gggcgaggcc cgcgccctgt gcccgccggc
    38341 tttgacggcg ggcatccttg cggccctggc ggagcaacag cccgcggccg tgacggcccc
    38401 gagcgcccgg cgctggcgga tgccggttgg cggcgtggcc gtggcagcat cggtgtgcat
    38461 ggctgcggta ttcggcctgc gcgcgctcgc gcccgagccc gcaggcatgc cgcaggtcgc
    38521 cgtggtcgcg tcgtcatcgt cagcgggcgc ctcgctcggc gaactcggac gcccgggcgg
    38581 cttgccggtg cccgtcacgg ggcccgcggt gccggtgggc ttcggagctg ccgtgccggc
    38641 tccgggcggc gctctgcagc acgcaggcgc caaccccgat cagctcgccg agcagcgctt
    38701 gcgcctgttc atggccgatc atgtccagaa cgcggcgctg aacaccaacc agggcatgct
    38761 gccctacgcg cgcgtggtct cgtacgaggc gccctgaggc gaaacggttt atttccatgc
    38821 gcccgctcgc cgtgctcgcc tgcctgctgc cgggttttct ctcggtcccc ttgcatgccg
    38881 tggaggccgg cgatccggct tcgcgcctcg acgcgatgtc gcgcagcttc cggtccctcg
    38941 actaccatgg cgtgttcacc tacgagcagg gacagtcgct cagctcggtg cgcatcgcgc
    39001 acgcggtggt cgacggcgtg gagcaggagc gcctggtgca tctcgacggc gagcagcgcg
    39061 agttcgtgcg ccgcggccac cgcgttgatt gcgagcacgc cggggatcgc ctgatgcggc
    39121 tggacccggc ggcacgcttc gcgcagcgcg cgcaaacggg cgtcgcgggc gcgcagctgg
    39181 gcgaccacta tgccatcgag ttcgacggta gcgagcgcgt ggccaaccac cgggggcagc
    39241 gtatccgcat cgttccgcgc gatccctacc gttacggcat gaacgtggtg ctggacgagg
    39301 cgagttcgct gctgctgaag tcggagacca cggacggtgc cggtcgcgtg ctcgagcgtt
    39361 tccagttcgt ggacctgcag atcggtgctc ccgtcgccgc cgccgagctg gcgcccgaga
    39421 cgcccggcgc gcatcacgcg gcagggcacg ccggggccga ggaacccgca cctttcgcat
    39481 ggacggttgc ctggctgccg gagggattcg tcgcgacggg acgcgaggtg cgcagcgccc
    39541 gcgccggggg gccagcggtc gaggtccaga gctataccga cgggctggcg gtgttcgcgg
    39601 tcttcgtcga gcgtaccgcc gagcgtccgc cgcatgcagg gcaggcctcg cgcggcgcca
    39661 cggtgtccta tgtcgtgccg cgcgaccagg agcacctggt tacggtggtg ggcgaaattc
    39721 ccgtcgagac tgcgcagctg attgccaacg gggtcaattt tccggacgca acgccatgat
    39781 gatcgagcgt gcacgcgtcg ttgcggtcga ggccgacgcc tgctgggtgg agaccatcgc
    39841 caggccgggt tgcggcgcct gcgcttcggg ccagggctgc ggcggcggcg tgatcggcag
    39901 gctgctcggt gagcgacgcc atcatctgcg cctcgtgctg ccggcgggac tgagtccggc
    39961 ggtcgacgac gaggtggaga tcggcgtgcg cgagcacagc gtgttgcgcg cctccgtgtt
    40021 tgcgtacctg ctgccgctgg ccggtatcgt ggccggggcc gtgacgggcg agcgccttgc
    40081 gccgccggga tccgccgagg cgtggtcgat ggcagccgcc gcggcagggt tcctgctcgc
    40141 cgtggccgcc gcgcgcgcgt tgtcgcgccg accgcgtgcg ggtgtcctcg agcccgagtt
    40201 gctcagggtg tttcccgcgg cgcgctccct cgcttgatca cttgttgtca tttccctgtc
    40261 tggagagcct atgaagcgca actggatgtc ggtttggtgt ggactcgcgt tgatgctggc
    40321 gctggccggc aagtcgtggt cggcggcatt gccggacttc accacgatcg tcgaaaagaa
    40381 ctcgggagcg gtcgtcaaga taatgagcac gcagaagtct ccggaggtgg aaggcttgcc
    40441 gcccgacatc gatccgcgcg cgctcgagca gatgccggag atcttccgtc acctgttcca
    40501 gtaccgcggc cagccgcagc gcgagcgcca gtccatgggc tcgggcttcc tggtctcggc
    40561 tgacggctac atcctgacca atcaccacgt ggtcgacggg gcggacgccg tgacggtgcg
    40621 cctggcggac cgccgcgagt tcgaggccag gatcgtcggc agcgacaagc gctccgatat
    40681 cgccctgctc aaggtcgagg cgagggatct gccggtagtg agcttcggca ataccgacga
    40741 actcgcggtg ggcgagtggg tggtggcgat cggctcgccc tttggcctcg actactcggt
    40801 caccgcgggc atcgtcagcg cgcgcggtcg cagcctgccc agcgacgggg aaaccggcaa
    40861 ctacgtgccg ttcatccaga ccgacgtggc gatcaatccc ggcaactccg gaggaccgct
    40921 gttcaatctc gatggcgagg tggtcggcat caattcgcag atctacacgc gcagcggcgg
    40981 ctcgatcggg ctttccttcg ctatccccat ttccgtggcc atcgacgtca tggagcagtt
    41041 gaaggcctcg gggcacgtgg tgcgcggctg gctgggcgtg ggcatccagg acgtggaccg
    41101 tgccctgg
//
LOCUS       DAORUQ010000006        40290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_595_length_40290_cov_6.190357, whole genome shotgun sequence.
ACCESSION   DAORUQ010000006 DAORUQ010000000
VERSION     DAORUQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 40290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40290
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_40290_cov_6.190357"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLW13_01160"
     CDS             complement(<1..636)
                     /locus_tag="PLW13_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69007.1"
                     /translation="MANLAAVVGVGQTKHKSKHKDVSMAGLVRQAAERALADAGCEWK
                     DIDALVLGKAPDMFEGVMMPELYLTDALGGNGKPVIRVHTAGSVGGSTAILAANYVQS
                     GVYNRVLTVTFEKQSESNATWALSPRMPFVPHVNAGAGGFFAPYMRQYKEKYGAPDYI
                     GCMVAVKDRLNACRNPYAHLQIPNIDLAMVQQSPMLWEPVRFLESCPSSDGA"
     gene            complement(640..1692)
                     /locus_tag="PLW13_01165"
     CDS             complement(640..1692)
                     /locus_tag="PLW13_01165"
                     /inference="COORDINATES: protein motif:HMM:NF005924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HQY69008.1"
                     /translation="MRDAAIIGYAQGPQYRYAGAANESELIMPVVRRVMEQVGVGQQD
                     IDFTCSGSCDYLQGAAFAFVEGLSALATVPPIKESHVEMDAAWAVYEALIKIWTGEAD
                     LCLVYGFGKSSPGELRTVMALQLDPYYHVPLWPDAISLAALQARAMLDSGAISERDMA
                     EVVARSRANARANPNAQLKGNPGIEELLAEPGFVSPLRRHDCPPITDGASAMIVASAD
                     VATRYCKRPAWVRGIDHRMETGILGARDLTRSPSTRIAGEKAGVARGKVDLAELHAPF
                     SHQEIILRRELGLADAVPVNLSGGALAGNTMMAAGLDRIGFVAERIINGEADRGVAHA
                     TSGPCLQQNLVAVLEA"
     gene            complement(1696..2160)
                     /locus_tag="PLW13_01170"
     CDS             complement(1696..2160)
                     /locus_tag="PLW13_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HQY69009.1"
                     /translation="MTDAREPVTGIEVPIYLNYEFTAGAALARFLASIREGRIMGQRC
                     PGCASVYVPPRGSCSRCGIPTEEEVEVKDTGVIEAFTIVHIPIPGNPIKPPFIAASIL
                     LDGTDMACLHLVSEVPTEQVHVGMRVQAVWRPREEWDYTFENIAWFKPAEGA"
     gene            complement(2172..2615)
                     /locus_tag="PLW13_01175"
     CDS             complement(2172..2615)
                     /locus_tag="PLW13_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY69010.1"
                     /translation="MASQPEILTKPFELGYTYTRSTGPVIGHFLTELREGRITGVRGS
                     DGGVIVPPAEYDPVSCAALTDIVAVADTGIVTTWCWVRQPTSHHVLQRPFAFALVKLD
                     GTAVPMLHVVDAGEESAMRTGMRVKARWAAERRGSITDIACFEPA"
     gene            2797..3897
                     /locus_tag="PLW13_01180"
     CDS             2797..3897
                     /locus_tag="PLW13_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY69011.1"
                     /translation="MKALVIGGTGPTGHHIVNGLIARGYTVEILHRGTHEVEEIPREV
                     VHHHADPYDPAVLREFFDARRYALCVATYGRLRAIAQESAGKVERFISAGGVPAYRGY
                     MHPQAFEPAGLPVPTGEDAAKVRGPEDDDKGWRILRTEETVFECHPGATHIRYPYVYG
                     RHQPIPCEWPIVRRILDGRAFIILPDGGLTLHHMGYAQNVAHGVLLAVDRPENARGRI
                     YNCADDEALTLRQRTEIIAAALGRAIEIVSMPWELAVPARPLVMQPLTTHRVLDTSRI
                     RVELGYADRVPAREALAITARELAATPPARGGVEEMVLQDPFDYSAEDRLRAAWESAL
                     ASMPAIAFDTEPGFTMSYSGPGGRARSNNSFT"
     gene            complement(3978..4592)
                     /locus_tag="PLW13_01185"
     CDS             complement(3978..4592)
                     /locus_tag="PLW13_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQY69012.1"
                     /translation="MSLTVVLSSFALLFLAEMGDKTQLMTMTLAHRYRALPVAIGVCA
                     AFLVLNLLAVAVGEVLFRYVPRSAVLLVAGAMFLFFAWKSWRDAGEPEEEAAERRGGG
                     GVVLSSFALIFVAELGDKTQLALIALVASTGDAWSVFTGATLALWSVSLLGVAVGSTL
                     LSRVPRAYMHRVAALMFAIFGVLALAQPLMSWLEPWVGMIVRPM"
     gene            4935..7586
                     /locus_tag="PLW13_01190"
     CDS             4935..7586
                     /locus_tag="PLW13_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY69013.1"
                     /translation="MNNVNHRRKALFVGIMGASALLLAEGTMAQDDVTNLGIEEMVVT
                     AQKREENVQAVPISISTLSANDIERRGVQSAADLMSTVPNMAGFQSPGARGNASIAMR
                     GVTSGSPANLSIDSSIGIYIDGFFLGKPLSAGMDVAELERIEVLRGPQGTLYGRNSTG
                     GAVNFITRKPSGEFGGKVSGTVGSDSLWGIKAAVDTPTLGTDGEGAGTLKGQFSAQTR
                     QRDGLYDSRSGAGVDQEFDDLDRDAYRVALRWEPNDAVTFDYAWDKSKLDEVNTVYSP
                     VGLTPYAIPTATNPGDRITRLNGYIAQGQAGLAGSGILAGPASSDPTFSRWLNSTIAM
                     RDAMLGINSDGERPSRGAVDAASEATNESEGHSLTVGWNFEELGFLGDVEFKSMTGWR
                     DAEARNLGDLDGFDNTVAPGGSGALNEVSAAAMYNYYAFGSILGGNASTAKLWSLIDT
                     FGGGFFVQDAQFDYEQFSQELQMVGSTERLQYAVGLYYFEDNGSFDNYRKAAIPFGGI
                     LTSAYDNETEASAFYSQFTYTPPVLDDKLALTVGYRYTEEDKGIRYRYLTDVNPATGL
                     GLRLPSGAINTAYTGELYQASTYGDQFDATFSNDSGNVTVAYQLTEDTNMFLRWATGY
                     RSGGFNGELYNNPFEEETIEQVELGVKSDVVPGLLRINASVFQFTYEDLQTSEIRLIN
                     NSPTSYYGNAGEADRWGSEIEAQLSPTDNLMIALSWTHMSGNFEKYPPVCGTGTFSGE
                     CLPTSDFAERGYSPDNSASLVADWVFVRTDWADFMAHVGAFWQDEMGTAALWAGTYAP
                     NPTATANARPYLYDHVTMDDRVVVNARVGIENVELSRGTLRAALWSNNLLDEDYPATG
                     INFGSLGMVTEAYGEPRTFGLDVTFEF"
     gene            complement(7691..8818)
                     /locus_tag="PLW13_01195"
     CDS             complement(7691..8818)
                     /locus_tag="PLW13_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY69014.1"
                     /translation="MSKFRRLLAYLDEQGLDGAALAAAVGIDARTLQCAAPSDVLPSL
                     YYGLLYTESVVRLQSHDLALPWAAGMGGKAFRLMACCVITCRTLGEALARASEFESLI
                     SPQLKGDRMTLERHGDLARLVYQHRLLDSQNRFIPRALHGTIWPLVLGRGSGLTVWHA
                     FCGWLIGRALEPEQVGVGVSALPEGYASKLAGIFRCPVRAVEGNSFLEFPAHFLDCKL
                     VHDAASLEDMLRTGPYQLMQMDHAPASTSAAIRSLLGNKFADGLPSFEEIAERLGMSS
                     SSLRRRLMSEQTSYQRLKDDCRRDAAIRLLQASELSVADIGERLGFTETSSFIRSFRA
                     WTGTTPRGFRDRTCLPRQAGGGALRVGQAALARGGERGQPP"
     gene            9023..10510
                     /locus_tag="PLW13_01200"
     CDS             9023..10510
                     /locus_tag="PLW13_01200"
                     /inference="COORDINATES: protein motif:HMM:NF005510.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69015.1"
                     /translation="MNSKTLPTRARRLTPDMDFFDGADVVVVGLGSAGASAAIEAAEQ
                     GATVLVLEAASAGGGTTAMAGGLIYMGGGTPTQKACGIEDTTEDMYEYLLMASGPNAD
                     PARVRLYADGSLAHYDWLLAQGMEYKPEYYARKHTNTPGDEGLIYTGNETCNEFRARA
                     APAPRGHKGKAQGEDGGRMLMEKLIASATAKGVRIRCDARALTAIVDEAGAVAGVVAR
                     IDGEERNIRARRGVVLCAGGFIMNNEMVQRHAPHLLHETVLNGNPNDNGMGIRIGMGA
                     GGAVMNMNEGFVCLPFYPPADLVEAIMINSKGQRFINEDCYHGRMGEAILRNYPEKHY
                     LVIDSRLFHTMERPPLGGFKVVATGDTVEELEAELALPAGTLAFTVDFFNRHARNGED
                     PLFRKHADYLVPLDNPPYAACDVTPGSGAFYPVFTLGGLHARPTGEVLTEDNVVVPGL
                     FTAGRNCCGLPRSGATYSSGMSIGDATFFGRLAGRSAAAAPARPH"
     gene            10521..11282
                     /locus_tag="PLW13_01205"
     CDS             10521..11282
                     /locus_tag="PLW13_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY69016.1"
                     /translation="MVELSLVSVSLLVVTGFIAGVINTLAGGGSNLTIPALMVMGLPA
                     DVANATNRVGVLLQSVAGVHGFHRHGKLDSHDTVPVLIPNLLGGLLGAVFAAAIPVSF
                     LKPLLLGTMIGMSLLILLRPAVIAPPPGTPPLSVRERPAAWAALFLAGVYGGFVQAGV
                     GFVLIAALAGTLRYDLVRTNALKMLCTGAFTLIALVVFIVDDLVLWLPGLVLGVSTMF
                     GAALGVRMALKVRQETLKWFLFLMTVCASAAALWH"
     gene            complement(11539..12216)
                     /locus_tag="PLW13_01210"
     CDS             complement(11539..12216)
                     /locus_tag="PLW13_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69017.1"
                     /translation="MTLKGKRIVITGAAGALGRGVSECARALGADTVLLDLAFPADAP
                     ADGSLRLCVDLGDAGATAACFGQIGPVDGLFNLAGGFAMGPRTWEVTDGEWAQMYQIN
                     VATMQNAVRAAVPGMVARGRGAIVNVGALGALKGQGHMSAYCAAKSVVMRLTESLSAE
                     LKHHGVNVNAVLPSMIDTPRNRADMPDADPSLWVSPQDLGNVICFLGSDAARAIHGAL
                     VPVAGLS"
     gene            complement(12281..13858)
                     /locus_tag="PLW13_01215"
     CDS             complement(12281..13858)
                     /locus_tag="PLW13_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69018.1"
                     /translation="MNHDRIHEPPLMVHMLIDGLNRYDERPCLFIGDAVLSYAEVRAT
                     ASRMHQALKAMGLGVGSRVAILSGNRPEVLTNLAAMQVTGCCGTPLHPLGSLDDHAYV
                     LEDAGIETLVYDPSAFDQRAAELKARLPGLKNLLAFGPSTVGVDYLALAAQFSAAPLL
                     APDIRPDDVSSINYTGGTTGKPKGVMSTARGGAYLTMLQMAEWEFPEELRMLMCTPLS
                     HAAAAFFIPVLQRGGAFYVPRGFTPDLFFDMVEQHRITATMLVPVMIYTLLDSPRAAT
                     ADLSSLETIFYGASAMSPARLREGIEKWGHVFYQYFGQSEAPMALANMKKAEHDLSKP
                     ERLASCGRPAPWVHLALLDDDNNPVAPGQPGELCVRAPLVMKGYHNLPEQTAEAFAGG
                     WLHTGDIARFDSEGFLYIIDRKKDMVVTGGFNVFPREVEDVISAHPAVAQVAVIGVPD
                     ERWGEAVKALVVLKPGVEQGDALREELVARVKDAKGSVQAPKSLDFVSAIPLTPIGKP
                     DKKALRTTYWAGAARKV"
     gene            complement(14040..15188)
                     /locus_tag="PLW13_01220"
     CDS             complement(14040..15188)
                     /locus_tag="PLW13_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69019.1"
                     /translation="MSASEQDTELALFRDSVRRFVKERVAPFYEEWESAGIIPRETWR
                     ELGAAGMLGTDIPLEYGGVGADFRFSVVVQQEIARAGFLALACNITVHSDICAHYVLN
                     AGTEEQKQRYLPKMVCGDCVGAIAMTEPGAGSDLQSIRASAQPDGEGWKINGAKTFIT
                     NGQHADLVITAAKTDLSAPGSKGISLLLVDADAPGFARGRNLEKLGLHAADTSELFYS
                     DVRVGPQALLGGLNRGFAVLMNELPRERLGLSIGAVAHARGALDITVAYVKERHAFGQ
                     PLAAQQNTRYRLAEMAAAITVHERFLDSCIASLMRHELDSATASVAKLSCTELEDQVV
                     DGCLQLFGGYGYMREYPISRFYADSRVQRIYGGTSEIMKELISRDVLK"
     gene            15452..16951
                     /locus_tag="PLW13_01225"
     CDS             15452..16951
                     /locus_tag="PLW13_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69020.1"
                     /translation="MNVPVDKSSPSRPRRLADVGRWDMETDVAIIGFGGSGAAAAIEA
                     SDQGAAVTIFDLASASGGSTALSSAEIYMGGNGGTRVQRACGLEDSTEDMYQYLMAAN
                     GPLADPDKVRAYCEGSLDHFNWLVGLGVPYKDTLYNQRAIMALTDDCLLYTGSEKAWP
                     YNRVAKPCPRGHNLQVEGDNGGPLFMKIMTENVEKRGVKVVFEARALTLIVDDERRVH
                     GVVVRIDQQERCVRARRGVILCAGGFAMNQEMLRKYSPLLLAASEPIGNPGDTGSGIQ
                     MGMAVGAAAINMHEGFVSLPYYPPSSLTWGILVNAQGQRFVNEDCYHGRVGYYCLQQP
                     DRRVYLIANVEDFGDYEKTSYLGAKFVATAEESVAELESELQLPEGTLQNTIEVFNRN
                     AAEGVDPLFHKTAEWLKPLELPLAALDCTPGHGAFYPFFTLGGLDTLPSGEVLTAERT
                     VIQGLYAAGRTACGVPRTASGYASGISVGDATFSGRMAGRAAAMAGAHD"
     gene            complement(16997..17773)
                     /locus_tag="PLW13_01230"
     CDS             complement(16997..17773)
                     /locus_tag="PLW13_01230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69021.1"
                     /translation="MAFHGKVALVTGGASGMGKVSALRLAAAGAQVAIFDMNEAALAE
                     TAAQSPNIRAWRCNVADWEEVQARVADVERELGPIDRLTHAAGIMPSHAAIDTTLEVA
                     RRLMEVNYFGTLHMIRAVVPGMVARNRGDVILFGSVSATALTPKLGAYAASKAAVNVF
                     GETLANELGHTALRIAVVMPPAVNTPLIQQSLDTDPAKALREAKKSGRLGDPAKIVEQ
                     IEQGLEKGRKAIYPGEAKLLDLWHALAPRLWWKTVLKFEQ"
     gene            17943..>18438
                     /locus_tag="PLW13_01235"
     CDS             17943..>18438
                     /locus_tag="PLW13_01235"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HQY69022.1"
                     /translation="MQRIGVLFVCLGNICRSPSAQAVLEDRLERRGLAAHFRVDSCGT
                     AAFNVGKAPDPRAIAAAERRGYRIAHQRARQVEDADFARFQHIVAMDRANLRTLQGWA
                     GPDFAGEIHLLLHFAPGATTEVADPYYGSPEAFDAALDAIERGVDGLLLALCARHGTE
                     LAAAA"
     assembly_gap    18439..18538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18646..19731
                     /locus_tag="PLW13_01240"
     CDS             18646..19731
                     /locus_tag="PLW13_01240"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQY69023.1"
                     /translation="MTDRTLEDLNVETIEELITPDQLKAEMPLTPAVSETVRSARGIV
                     RDILDRQDHRLFLVVGPCSIHDTGAAMEYAHRLRRLAEEVQDTLYLVMRVYFEKPRTT
                     TGWKGLVNDPHLNDSFRIEEGLHIARKLLIDITGIGLPTATEALDPISPQYLQDLIAW
                     SAIGARTTESQTHRELASGLSCAVGFKNGTDGGLTVAVNALKSVNSPHRFLGINIEGK
                     VAVIHTRGNRHAHVVLRGGTNGPNYDSVHVAVCERELEKAGIAPNIVVDCSHANSNKD
                     HALQALVLDNVANQILEGNRSIVGAMLESNLEAGNQPIPSDLAKLRYGVSVTDACMDW
                     NSTEQAIRSMHEKLRRILPQRSGAIGS"
     gene            complement(19807..21489)
                     /locus_tag="PLW13_01245"
     CDS             complement(19807..21489)
                     /locus_tag="PLW13_01245"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQY69024.1"
                     /translation="MVQPLPPDIAIARAATMLPIDAIAARAGIPADALIPYGRSKAKI
                     DRAFLEALDGRPDGKLVLVTAVSPTPAGEGKTTVAVGLADGMNRIGCNTLLALREPSL
                     GPCFGMKGGAAGGGFAQVVPMEDINLHFTGDFHAITAAHNLLAAMVDNHLYWSNDLRI
                     DPRRVSWRRTVDMNDRSLRDICVGLGGAANGLVREAGYDITPASEVMAIFCLARDLAD
                     LERRLGNIIIGYRRDLSPVYAREVNAAGAMTVLLRDAMQPNLVQTLENNPVLIHGGPF
                     GNIAHGCNSLIATRSALKLADYVVTEAGFGADLGAEKFFDVAARTGGLHPDAAVLVAT
                     VRALKMHGGQRKSELGRENLEALRAGCANLARHVRNIGLFGVPVVIAINRFDADTDAE
                     IALLKEQAARLGCEAVESTHFRDGGGAGAEALARAVQRLADSGLSQFRTLYDDDMPLW
                     DKVCCVARTIYGAGKVVADQKVLDRFRQYDAIAGRFPVCIAKTQYSFSTDPGLLGAPT
                     DHAVSVRDVVLANGAEFAVVLCGDIMTMPGLPRRPAAEGIRLNGSGEIEGLF"
     gene            complement(21549..23828)
                     /locus_tag="PLW13_01250"
     CDS             complement(21549..23828)
                     /locus_tag="PLW13_01250"
                     /inference="COORDINATES: protein motif:HMM:NF023282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HQY69025.1"
                     /translation="MNTRRKTLWIAIVGAGLAMAGAAQAQDEYGFEDTSAAKPRVVYE
                     NFIELGGGYTDDGNGKFGEYTSGLTDAFQDEGGFPVGALRLSGGDDATARAWEVSAGV
                     GNGRALEASYGVQNDFGISLYGDRIEKTEYGEAGTVYGGASDTMLLPPTYKFTTSFPI
                     PAAPPGSYDAANFSTQDIRHERQIFGLEGFKNIGDAWTVSLGFESQEKSGEDVFGGNQ
                     GFSGTALVPEELDTEHQQMRARIDYADRCLQHGFELYLSEFENGNDAIAFQNAIVPGS
                     FVPNNASGTFRPLGIEQVASDPDNRYLRLGMDGGYSMGLMTRLSWFADWSRGEQDEDF
                     LPVVLDTNYYPSVNPVFPFSNLDGEVERANVKLSLAGRPFSRFDYRVQYAYKDRDARH
                     DPLPVNELSYTYNSSAPGFTSKVYDKQTQTLGAEAGYRFGGRVKLRGGYEYEEADRDT
                     DELEYDSVARLYSPASFTDSTTEDKVWAQLSIATIEALTIKLRGEYNVIEADLSDETG
                     EVISPGGIPRRGTPVFLLDRDQEVYEIEANYALGAAASVYARYDVVRDDYDNDTFGLE
                     KRDSEIWNVGFSWSPSDAVALSAYASRESYEFEQIGRQMNQGGPTPYIDWGLDTEDDV
                     DAFGLGMDWAVVEDRFSLSADVAYLEADSSYASEFLGSTYPAGTALPLFGDTPDSQDE
                     LLRFSLSGVYRWNERIDVSGRYIYETRDAEDWGWSDQVVGPATPADPAQASARYLAFA
                     WARPDYSSQLVVMSVRYKF"
     gene            complement(23863..24789)
                     /locus_tag="PLW13_01255"
     CDS             complement(23863..24789)
                     /locus_tag="PLW13_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HQY69026.1"
                     /translation="MRNLRLTRLFGVIVVSLACQAGGAAAAEDRAALPPSTTYSEGVK
                     VCTECHDDEHATAIMSGPHGMAADPRTPAATKGCESCHGPGGEHARQEKKFRVAQTLE
                     GHSEEDAAERAEVCMNCHGKTAQKHFLTSEHSRADVACSDCHDVHSRNDKVLNRVTQT
                     EVCVDCHQDQKANMNKFSRHPIREGKVACTDCHNPHGAKGEHMLVEATTNETCYKCHA
                     EKRGPFLFEHEPVQDDCSECHNPHGSVNDQLLTARPPFLCQQCHNDTRHPGTVYANAN
                     VASSNRAIGRGCTNCHTNVHGSNHPSGVTFRR"
     gene            complement(24878..25789)
                     /locus_tag="PLW13_01260"
     CDS             complement(24878..25789)
                     /locus_tag="PLW13_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69027.1"
                     /translation="MPHQSPRPIVPRSRRLIACAFVCAMVLPAASWAVDWGKTAAAEV
                     SLFYPGQMSWEKVLTASSHKGATKFRAGETCHDCHRDEEAEMGASQAQLTGFAGRNAV
                     TVQLRAAVQGDSLHLQMSGPAVDGKAPSVAIMLGNEALKSTAQAGCWAACHDDAPGMA
                     SDSGQRLGKYLPRSRSRNTATGGGDSVRPQAELDVALAAGEFLDVLEIEASGTGERGH
                     VLDKLHMGELVGAASMRIENGRWVAEIQRPLQAAGSGQIALEAGKVYHLGIALHDPGS
                     EGREHLVSLARSLSIGSGSADIVAASQ"
     gene            complement(25881..26468)
                     /locus_tag="PLW13_01265"
     CDS             complement(25881..26468)
                     /locus_tag="PLW13_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HQY69028.1"
                     /translation="MSIFREPGSRRGRGRGRTLVLLAAGALLGIIGVVAFNTTLEATS
                     TDEFCLGCHNHGIPYKQHQLTVHYTNPHGVVAGCSDCHVQHEFVPKMTRKIEAAREVW
                     GHLRGVIDTDEKYLAHQPAMKARELARFRASDSATCRSCHDVARMDLEAQSPKARREH
                     ARLGSGAKTCVDCHEEAGHAALAPATAEQEQSFDF"
     gene            complement(26653..27234)
                     /gene="nfuA"
                     /locus_tag="PLW13_01270"
     CDS             complement(26653..27234)
                     /gene="nfuA"
                     /locus_tag="PLW13_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HQY69029.1"
                     /translation="MITITESAQEYLVGLLAKQDPEVRGIRVFINDPGTPRAETCIAY
                     CRDADIKSDDERREFNGFAAWFEARSLPFLADALVDYAADRMGGQLTIKAPNAKVAKV
                     DADSSLEDRINYILYNEVNPQLAAHGGEVRLVELLEDGVAVLQFGGGCQGCGMVDATL
                     KHGVEKTLLEQLSDLKGVRDVTDHSVRTNAYFT"
     gene            27417..31094
                     /gene="metH"
                     /locus_tag="PLW13_01275"
     CDS             27417..31094
                     /gene="metH"
                     /locus_tag="PLW13_01275"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQY69030.1"
                     /translation="MQTDQTLQQAIAERILVLDGAMGTMIQGYGLQEADFRGTRFAGH
                     PVPLRGNNDLLALTRPDVIREIHAAYLAAGADIIETNTFNSTAVSQADYELQHIVREL
                     NEAGARVARLAADEFTAREPNKPRFVAGVLGPTSRTASLSPDVNDPGFRNITFDGLAA
                     DYRVAIEGLIAGGADLLLIETVFDTLNAKAAIFACEQFFEEHGRRWPVMISGTITDAS
                     GRTLSGQTAEAFWYSVAHARPLAIGLNCALGAEQLRPHVLALAKIADTAVSAHPNAGL
                     PDEFGGYNQDAATMAAIVGEFADSGLLNIVGGCCGTTPAHIRAIAERVAGVAPRRIGR
                     APAALRLAGLEPLVVTADSLFVNIGERTNVTGSARFARLIRDQDYDAALDVARQQVEA
                     GAQLIDVNMDEGMLDSRAAMERFLNLVASEPDISRVPVMIDSSKWDVIESGLRVVQGK
                     SVVNSISLKEGEQEFLAHARLARRYGAAVVVMAFDEQGQADTLERKIAICTRCYELLV
                     HEVGFAPEDIIFDPNVFAIATGIEEHANYAVDFIECCRHIKQHLPGALVSGGISNVSF
                     SFRGNDRVREAIHAVFLYHAIRAGLDMGIVNAGQLAIYDEIPAELRERVEDVVLNRRA
                     DGTERLLEIAAKYAGGAAIAAVEDLEWRGWPVERRLEHALVKGINSWIEEDTEAARVA
                     AARPLDVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVACLLPYMNQDRAEG
                     ERTTNGTVLLATVKGDVHDIGKNIVGVVMQCNNFEVIDLGVMVSCERILAAAREHDVD
                     VIGLSGLITPSLDEMVHVASEMQRLGFDIPLLIGGATTSKAHTAVKVEPAYAAGPTVY
                     VPDASRGVTVVSQLLSDTQRAEFITERRHEYARIRERNANRPARAALLSYSDAAANAP
                     ALDWAAYQPPRPAFTGVRVFDDYPLTELIDVIDWTPFFISWDLAGKYPAILEDATVGA
                     AARNLFDDAQKLLRRIIDEKLLVARAVVGFWPAARLGSDDIAVYTDETRSARLEVLHC
                     LRQQQAQADGRPNLSLADYVAPAGGPPDWLGAFAVSTGFGADELAARFQAEHDDYNSI
                     MTKALADRLAEALAEHMHRLVRRELWGYAPTESLSNEDLIRERYRGIRPAPGYPACPD
                     HTEKRPLFRLLDAERSTGITLTENFAMHPAAAVSGWYFSHPDSRYFAVGKIGRDQLED
                     VAARKGMPVDEMERWLRPNLA"
     gene            31141..31392
                     /locus_tag="PLW13_01280"
     CDS             31141..31392
                     /locus_tag="PLW13_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HQY69031.1"
                     /translation="MSQQSPGRENGGGDEGRKSVPLLGVVKSIAAAAFGVQSSRNREA
                     DFTHGNYRHFIVGGIIFTVIFVLTVAFVVSLVLKNAGAS"
     gene            complement(31416..31550)
                     /locus_tag="PLW13_01285"
     CDS             complement(31416..31550)
                     /locus_tag="PLW13_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69032.1"
                     /translation="MANPDNSIEISAEEREADSKVDAISAILLVAIAVFTALFWISGQ
                     "
     gene            31818..33482
                     /locus_tag="PLW13_01290"
     CDS             31818..33482
                     /locus_tag="PLW13_01290"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HQY69033.1"
                     /translation="MYRYDDLDQRMLDDRVRQFRGQTERYLRGELAENEFRPLRLQNG
                     LYVQRLAPMLRVAIPYGMLASRQLRKLAHIARHFDKGYAHFSTRQNVQFNWPRLEEVP
                     DILAELASVQMHAIQTSGNCIRNTTADQFSGVAPDEVEDARPYCELIRQWSTFHPEFS
                     FLPRKFKIAVSGSRADRAAARVHDIGLIIVHDAAGNTGFEVLVGGGLGRTPIIGTTIR
                     EFLPVADLLSYLEAILRVYNQYGRRDNLYKARIKILVKALGAEEFRARVEREWEITRQ
                     RMPALGAAQIEHARSYFTAPPYRPVDDAAAESLLAGQRAANRAFDRWMSRNVSGHRVP
                     GYAIVTLSLKPTGIAPGDATDRQMELIADLADSYSFGEIRVAHEQNLVLADVEKEKLL
                     ALWEAARDAGLATPNIGMLTDIICCPGGDYCSLANAKSIPVAEAIQRRFDDLDYLHDL
                     GDIHLNISGCMNACGHHHVGQIGILGVDKKGEEFYQVSLGGDAGTEAALGTILGPSFA
                     QEAIPAVVAQLLDVYVARRIDGETFLETYRRIGIEAFKEAVYGAAH"
     gene            33466..33996
                     /locus_tag="PLW13_01295"
     CDS             33466..33996
                     /locus_tag="PLW13_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HQY69034.1"
                     /translation="MARLIKDGAVVQDEWTRLGADFDTDATLPAGKLIVPLHLWEARG
                     EELRARSEPLGIWIDSSQHPEAVGDALAYLQLIAINFPVFSDGRGYSYARLLREKYGF
                     SGELRAMGDVLRDQLFLLRRCGFDSFEIRADRDAADALAGLGDFAHVYQGATVDPGTP
                     LLLRGAQEARPVRANA"
     gene            34133..35302
                     /locus_tag="PLW13_01300"
     CDS             34133..35302
                     /locus_tag="PLW13_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY69035.1"
                     /translation="MEQSPVTFSFFLIFTGAAAVASLALYTRQPLLIAYIVLGALIGP
                     YGLALITNLTLLQDIAHVGIIFLLFLLGLDMQPRALLTTMRNSTLVALASALCFALIG
                     AGVAYAFGFSRTEALITGLAMMFSSTIIGIKLLPTTVLHHRHTGELMVGLLLLQDMIA
                     ILVLVALLGSRSGDFEGGALLLSLLALPLLIALAVVFVRLVLMRLIQRFDRFHEYIFL
                     LAIGWCLGLAELAALMKLSPEIGAFVAGVSIASSPISQYIAISLKPLRDFFLILFFFS
                     LGGSFDLGVLGSIYVAAIVLSLLMLAAKPLVFRFLLGRLSETPALAWDVGLRLGQNSE
                     FSLLIAYMAAGFGMIGKEASHLIQATAIITFVASSYIVVLNCPNPIAISDRLRRD"
     gene            35430..37346
                     /gene="speA"
                     /locus_tag="PLW13_01305"
     CDS             35430..37346
                     /gene="speA"
                     /locus_tag="PLW13_01305"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HQY69036.1"
                     /translation="MKANGKPNWTPADSAELYGIRSWGAGYFDLDDDGSVTVSVTLEG
                     KRVSVSIPDIIAGMQQRGLQMPVLLRIENLLDSQITLLNETFANAIASLNYRGSYRGV
                     FPIKVNQQCQVIEEIARFGAAYGHGLECGSKAELLIALASLDPDASHIVCNGYKDEEF
                     ITLALQSLKLGYRCIFVIETPAELPVILECSRRIGVKPILGLRVKLASKVGGYWNESS
                     GDRSLFGLTTSQIVELVDSLREHDMLDCLQLLHYHLGSQIPNIRDIRSGVLEACRYYV
                     DLVKEGAALGYLDLGGGLAVDYDGTQTNYEYSKNYSLDEYCSDIVESIMTTLDPEGIA
                     HPVIVTESGRATVAYSSVLLFNILDVTRFESGTLPQQIDPTEHELIRNLAEVLTSVSG
                     KNLQECHNDAVYYRDEIRDLFKRGQIRLRSLSLSEKLFLEIVRSIVRESQSAKRLPPG
                     LEGLEDSLSDIYYGNFSVFQSLPDVWAIEQVFPVMPVHRHIEKPTRQAIIADITCDSD
                     GKIDQFISPRGLQKTLAVHPLREGEPYYFGVFLVGAYQETLGDLHNLMGDTNVVSVRI
                     NADGSFDFVREMSGDSIADVLSYVEYQPQQLLEQFRRTAERAVRNGRIEPAQRQHLLE
                     SFNASLRGYTYFED"
     gene            37362..38555
                     /locus_tag="PLW13_01310"
     CDS             37362..38555
                     /locus_tag="PLW13_01310"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HQY69037.1"
                     /translation="MAKVIIVGAGGVGQVVTHKCAQVPEVFSEIVLASRNEAKCRHIA
                     SQIARPIATAQVDANNVAEMAALIRREKPDLVINVALPYQDLPIMDACLETGVDYLDT
                     ANYEPPDEARFEYKWQWAYQERFASRGIMGLLGSGFDPGVTNVFTAWLHKHEFDVIDE
                     LDIIDCNAGEHGQAFATNFNPEINIREVTARGKYWDQGAWQETDPLSVSRMFDFPEGI
                     GPKKIYLMYHEELESLVKHFPKVRRARFWMTFSDNYLNHLKVLQNVGMTRIDPVIYNG
                     QEIVPLQFLKAVLPDPSSLGPLTKGRTCIGCLARGTKDGKPKTVFVYNICDHEACYRE
                     VQSQAISYTTGVPAMIGAKMMLTGQWRGAGVFNMEQFDPDPFMEALNQHGLPWDVMRL
                     DGGMD"
     gene            38566..39726
                     /gene="nspC"
                     /locus_tag="PLW13_01315"
     CDS             38566..39726
                     /gene="nspC"
                     /locus_tag="PLW13_01315"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HQY69038.1"
                     /translation="MRHFHRFDARRVPSPCFVVDEVALEENLQLLAHVQRESGAKILL
                     ALKAFSMFSMAPLVRRYLAGTCASGLHEARLGREEFGGEVHTFCAAFTEPDLREVLAL
                     SDHVVFNSFGQLGRFRPLLEAALAARPGLRIGMRVNPEHSEGAVPLYDPCAPCSRLGV
                     PLSQMRADQLELLSGLHFHTLCEQDLPPLARTVQAFEQKFGEFIPRMKWINFGGGHHI
                     TRPGYQVDELIRLLVDFRERHGVQVYLEPGEAIAYQCGVMVSEVLDLTWNRMPQAILD
                     TSATCHMPDVIEMPYRAMITGAGEPGEHPHTYRLGGQTCLAGDVIGDYAFPEPLSLGQ
                     RLVFEDMAYYTMVKTTTFNGINLPSIAIWNSHSDEVRVVRRFGYEDFRSRLS"
     gene            complement(40070..>40290)
                     /locus_tag="PLW13_01320"
     CDS             complement(40070..>40290)
                     /locus_tag="PLW13_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY69039.1"
                     /translation="NDYQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIVAINKD
                     EDAPIFQVADYGLVADLFQAVPELAAAL"
BASE COUNT         6756 a        13788 c        13044 g         6602 t
ORIGIN      
        1 ggcgccgtcc gaggagggac agctttcgag gaatcgcacg ggctcccaca gcatcggcga
       61 ctgctgcacc atcgccagat cgatgttcgg tatctgcagg tgcgcgtagg ggttcctgca
      121 ggcgttcagc cggtccttga ccgccaccat gcagccgatg taatcgggcg cgccgtattt
      181 ctccttgtac tggcgcatgt agggcgcgaa gaagccgccg gcgccggcat tcacgtgcgg
      241 aacgaagggc atgcgcggcg acagcgccca ggtcgcgttc gattccgact gcttctcgaa
      301 ggtcacggtg agcacgcggt tgtacacgcc gctctgcacg tagttcgcgg caaggatcgc
      361 ggtggatcca ccgacgctgc ccgcggtatg cacgcggatg accggcttgc cgttgccgcc
      421 cagcgcatcc gtgaggtaca gctcgggcat catcacgccc tcgaacatat ccggcgcctt
      481 gccgagcacc agcgcgtcga tgtccttcca ctcgcagccg gcatcggcca gcgcgcgctc
      541 cgcggcctgg cgcaccagcc ccgccatcga aacgtccttg tgcttggatt tgtgcttggt
      601 ctggccgacg ccgaccactg ctgcgagatt cgccatgatt caggcctcca gtaccgcgac
      661 caggttctgc tgcaggcaag gccccgaggt ggcatgcgcc acgccgcgat cggcctcgcc
      721 gttgatgatg cgctcggcca cgaagccgat gcggtccagc cccgccgcca tcatcgtgtt
      781 gccggccagc gcgccgcccg agaggttcac gggcaccgcg tcggccaggc cgagctcgcg
      841 gcgcaggatg atctcctggt ggctaaacgg cgcgtgcagc tccgccaggt ccaccttgcc
      901 gcgcgccaca cccgccttct cgcccgcgat ccgcgtcgag ggcgagcgcg tgaggtcgcg
      961 cgcaccgagg atgcccgtct ccatgcgatg gtcgatgccg cgaacccacg ccgggcgctt
     1021 gcagtagcgc gtcgccacgt ccgcggaggc gacgatcatc gccgaggcgc cgtcggtgat
     1081 cggcgggcag tcgtgacgcc tgagcggcga gacgaagccc ggctccgcca gcagttcctc
     1141 gatgccggga ttgcccttga gttgcgcgtt cggattggcc cgcgcgttgg cgcggctgcg
     1201 cgccacgacc tcggccatgt cgcgctcgct gatggcgccg ctgtcgagca tcgcccgcgc
     1261 ctgcagcgcc gccaggctga tcgcatcggg ccaaagcggc acgtggtagt acgggtccag
     1321 ctgcagcgcc atcaccgtgc gcagttcgcc cggcgaggac ttgccgaagc cgtagaccag
     1381 gcacagatcc gcttcgccgg tccagatctt gatcaatgcc tcgtagacgg cccatgcggc
     1441 gtccatctcg acgtgcgact ccttgatcgg cggcaccgtc gcgagcgcgg acaggccctc
     1501 gacgaatgcg aaggcggcgc cctgcaggta gtcgcagctg cccgagcagg tgaagtcgat
     1561 gtcctgctgg cccacaccaa cctgttccat cacccggcgc accaccggca tgatcagctc
     1621 gctctcgttg gccgctcccg cgtagcggta ctgcggaccc tgtgcataac ctatgatcgc
     1681 tgcgtcacgc atgcttcatg ctccctcggc cggcttgaac caggcgatgt tctcgaaggt
     1741 gtagtcccat tcctcgcgcg gcctccagac cgcctgcacg cgcatgccga cgtgcacctg
     1801 ctcggtcggg acctcactga ccaggtgcag gcaggccatg tcggtgccgt cgagcagtat
     1861 gctcgccgcg atgaacggcg gcttgatcgg gttgcccggg atcgggatgt gcacgatggt
     1921 gaaggcctcg atcacgccgg tgtccttcac ctccacctct tcctcggtcg gaataccgca
     1981 gcgcgagcac gagccgcgcg gcggcacgta cacgctggcg cagcccgggc agcgctgccc
     2041 catgatgcgc ccctcgcgga tactggcgag gaagcgcgcc agcgcggcgc cggcggtgaa
     2101 ttcgtagttc aggtagatcg gcacctcgat gccggtcacg ggctcacgtg cgtctgtcat
     2161 cgtggttctc ctcaggccgg ctcgaagcag gcgatgtccg tgatcgagcc gcgccgctcg
     2221 gcggcccagc gcgccttgac gcgcatgccg gtgcgcatcg ccgattcctc gccggcatcg
     2281 accacgtgca gcatcgggac cgcggtgccg tcgagcttca ccagcgcgaa ggcgaagggg
     2341 cgctgcagca cgtggtgcga ggtcggctgc ctgacccagc accaggtggt cacgatgccc
     2401 gtgtcggcaa ccgcaacgat gtccgtcagc gctgcgcagc tcaccggatc gtattcggcc
     2461 ggcggcacga tcacgccgcc gtcggagccg cgcacgccgg tgatgcgccc ttcccgcaac
     2521 tcggtgagga agtgcccgat cacgggcccc gtcgagcgcg tgtaggtgta cccaagctcg
     2581 aagggcttgg tgaggatctc cggctggctc gccatgtatc gctctcctgg aactacgttg
     2641 aaatggcatg gtgcacgctt gggccagggc tggctatggc atcaccgcgc gcattgcgct
     2701 gacaaaatta atcagtcgca acctttgtca cgcgcgacgc cgccccggtt tatagtccag
     2761 cgcatcgttt cgcaacatta caaaggatcc acctgcgtga aggcactcgt catcggcggc
     2821 accgggccca ccgggcatca catcgtcaac ggcctgatcg cgcgcggata cacggtcgag
     2881 atcctgcatc gcggcaccca cgaggtcgag gagattccgc gcgaagtcgt gcatcaccac
     2941 gccgacccct acgacccggc cgtgctgcgc gagttcttcg acgcgcgccg ctacgcgctg
     3001 tgcgtggcga cctatggccg gctgcgcgcc atcgcgcagg aaagcgcggg caaggtggag
     3061 cgcttcatct cggccggcgg cgtgcccgcc tatcgcggct acatgcaccc gcaagccttc
     3121 gaaccggccg gcctgcccgt ccccaccggc gaggatgccg ccaaggtgcg cggaccggaa
     3181 gacgacgaca agggctggcg catcctgcgc accgaggaga cggtgttcga atgccatccc
     3241 ggggccacgc acatccgcta tccctacgtg tatggccgtc accagcccat tccgtgcgaa
     3301 tggccgatcg tgcgccgcat cctcgacggg cgcgcgttca tcatcctgcc cgacggcggc
     3361 ctcacgctgc atcacatggg ctacgcgcaa aacgtggcgc acggcgtgct gctcgccgtg
     3421 gaccggccgg agaacgcgcg cgggcgcatc tacaactgcg ccgacgacga ggcgctcacg
     3481 ctgcgccagc gcaccgaaat catcgccgcg gcgctggggc gcgccataga gatcgtgtcg
     3541 atgccgtggg agctcgccgt gccggcacgc ccgctggtga tgcaaccact gaccactcac
     3601 cgcgtgctgg acacctcgcg catccgcgtg gaactgggct acgccgaccg ggtgccggcg
     3661 cgcgaggcgc tggcgatcac ggcgcgcgaa ctggcggcga caccgccggc gcgcggcggc
     3721 gtcgaggaaa tggtgctgca ggatcccttc gactacagcg ccgaggaccg cctgcgcgcg
     3781 gcctgggaaa gcgcactggc gtcaatgccg gcgattgcat tcgacacgga acccggtttc
     3841 acgatgtcct acagcggccc cggcgggcgg gcacgctcga acaactcctt cacgtaggtc
     3901 cggattcatc cggacaggcc ccgtgtctgc ggattcatcc ggacacgggt tcggcacagg
     3961 ccggacccgc caatcattca catcggccgc acgatcatcc ccacccacgg ctcgagccac
     4021 gacatcagcg gctgcgcgag cgcaagcacg ccgaagatcg cgaacatcag cgccgccaca
     4081 cggtgcatgt acgcccgcgg cacccgcgag agcagcgtgc tgcccaccgc caccccgagc
     4141 agcgacacgc tccacagcgc cagcgtggcg ccggtgaaca ccgaccaggc gtcaccggtg
     4201 ctggcgacca gcgcgatcaa cgccagttgt gtcttgtcac cgagttcggc cacgaagatc
     4261 agcgcaaagc tgctgagcac caccccgccg ccgccgcggc gctccgcggc ctcctcctcg
     4321 ggctcgccgg catcgcgcca cgacttccag gcgaagaaca ggaacatcgc gccggcgacc
     4381 agcagcaccg ccgagcgtgg cacgtagcga aacagcactt cgccgaccgc aaccgcgagc
     4441 aggttcagca ccaggaatgc cgcgcagacg ccgatcgcga ccggcagcgc gcgatagcga
     4501 tgcgccagcg tcatcgtcat cagctgggtc ttgtcgccca tctcggcaag gaacagcagg
     4561 gcgaaggagg agaggacgac ggtgaggctc atcggatcgg gatccatctg cggttgacgc
     4621 ggcggcatgg tagcgcagcg ccgccggtgc cgcactcgaa gctgcatgat ggcgagggtt
     4681 tcgggcgtcg cgcgcctctt gcaccaggag ggcgatccgc ccggaccagc cggcaccgat
     4741 aggcagcaca gttcgcgcgg catgcaccgc agcgttcgaa ccggggcatt tcaggtgatt
     4801 tttcacacac ttggagctga actttgcatc gaaaggacgc aagctgcggc accacaatga
     4861 cggtcagcgt catgtgcgct acgctcggct gagcaatgca gtcggcctca tctttccaaa
     4921 ggagaacaac ggggatgaac aacgtgaacc atcgcaggaa agcgcttttc gtgggcatca
     4981 tgggcgccag cgccctgctg ctcgcagaag gcaccatggc ccaggacgac gtcaccaacc
     5041 tcggtatcga ggaaatggtc gtcaccgcgc agaagcgcga ggagaacgtg caggcggtgc
     5101 cgatctcgat ctccacgctc agtgcgaacg atatcgagcg ccggggcgtg cagagcgccg
     5161 ccgacctgat gagcaccgta ccgaacatgg caggcttcca gtcgcccggc gcacgcggca
     5221 acgcctcgat cgcgatgcgc ggtgtcacca gcggcagtcc ggccaacctg tcgatcgact
     5281 cctcgatcgg aatttacatc gacggctttt tcctcggcaa gccgctcagc gcgggcatgg
     5341 acgtggctga actcgagcgc atcgaggtgt tgcgcggccc gcaaggcacg ctgtatggcc
     5401 gcaattccac tggcggtgcg gtcaatttca tcacgcgcaa gccttcgggc gaattcggcg
     5461 gtaaggtcag cggtaccgtg ggcagcgaca gcctttgggg catcaaggct gcggtagaca
     5521 ccccgacgct cggcaccgac ggcgagggcg ccggcacact caaggggcag ttctcggcgc
     5581 agacccgcca gcgtgacggc ctgtacgatt cccgctccgg ggcaggcgtc gatcaggagt
     5641 tcgacgacct cgaccgcgac gcctatcgcg tggcgctgcg ctgggaaccg aacgacgcgg
     5701 tgaccttcga ctacgcttgg gacaagagca agcttgacga ggtgaacacg gtttattccc
     5761 cggtcggcct cacgccttac gccatcccga cggccaccaa tccgggtgac cgcatcacgc
     5821 ggctcaatgg ttatatcgcc cagggtcagg ccggtctcgc gggcagtggc attctggctg
     5881 gcccggcatc cagcgacccc accttctcgc gctggctgaa ctcgacaatc gccatgcgcg
     5941 acgcgatgct cggcatcaac agcgacgggg agcgtccgag tcgcggcgca gtggacgcgg
     6001 cctcggaggc gaccaacgag agcgaaggac acagcctgac cgtggggtgg aactttgagg
     6061 aactcggatt cctcggtgac gtcgagttca agtcgatgac cggctggcgc gatgcggaag
     6121 cgcgcaatct cggtgacctc gacggcttcg acaacacggt tgcgcccggc ggctccggcg
     6181 ccctgaacga agtcagcgcc gcggcgatgt acaactacta cgcctttggc agcattctgg
     6241 gcggcaacgc ctccacggcc aagctgtgga gcctgatcga caccttcggc ggtggcttct
     6301 tcgtgcagga cgcgcagttc gactacgaac agttctcgca ggaactgcag atggtgggca
     6361 gcaccgagcg tctgcagtac gcggtggggc tctactactt cgaggacaac ggcagcttcg
     6421 acaactatcg caaggcggcc atcccgttcg gcggtatcct gacctcggca tacgacaacg
     6481 agaccgaggc ctcggccttc tattcccaat tcacctacac cccgccggta ctcgacgaca
     6541 agctcgcgct gaccgtcggc taccgttaca cggaggaaga caagggtatc cgctaccgct
     6601 acctgacaga cgtgaatccc gccaccggct tgggcctgcg cctgcctagc ggcgcgatca
     6661 acaccgcata caccggcgag ttgtaccagg cctcgaccta cggcgaccag ttcgacgcta
     6721 cattcagcaa cgactcgggc aacgtcacag tggcctacca gctgactgaa gacaccaaca
     6781 tgttcctgcg ctgggcgacg ggctatcgca gcggcggctt caacggcgag ctctacaaca
     6841 acccgttcga ggaggagact atcgagcagg tcgagttggg cgtgaaatcg gacgtcgtgc
     6901 ccggcctgtt gcgaatcaac gcctcggtgt tccagttcac ctatgaagat ctgcagacca
     6961 gcgagatccg gctgatcaac aactcgccga cttcctacta cggcaatgcc ggcgaagccg
     7021 atcgctgggg ttcggaaatc gaagcgcaac tctcgccgac cgacaacctg atgatcgccc
     7081 tgagttggac ccacatgagc ggcaacttcg agaagtaccc gccggtgtgc ggcaccggta
     7141 ccttctcggg tgaatgcctg ccgaccagcg atttcgccga gcgcggctac tcgcccgaca
     7201 actcggcctc gctggttgcc gactgggtgt ttgtgcgcac cgactgggct gatttcatgg
     7261 cccacgtcgg ggcgttctgg caggacgaga tgggtaccgc agctctgtgg gccggcacct
     7321 atgcaccgaa cccgacggca accgcgaacg cgcgtccgta cctgtacgac catgtcacga
     7381 tggacgatcg cgtggtggtc aacgcccgcg tcggtatcga gaacgtggag ttgtcgcgcg
     7441 gtacgctgcg cgccgccttg tggtccaaca acctgctgga cgaggattac ccggccaccg
     7501 gtatcaactt cggctccctc ggcatggtca ccgaagcgta tggtgagccg cgcaccttcg
     7561 gcctggacgt gaccttcgag ttctgattcc ggtcggctac gaaacgactt tcaagggccc
     7621 ctcggggccc tttttcttgt ccggctgttt ctgcagacca tccgtcgcag gcatcgagtg
     7681 cggcggacgc tcagggcggc tggccccgct cgccaccgcg cgccagggcc gcctgcccga
     7741 cgcggagcgc accgccgcct gcctgccgtg gcaggcaggt acggtcgcga aaaccacgtg
     7801 gcgtggtgcc cgtccaggcg cggaacgagc ggatgaagga actcgtctcg gtgaatccca
     7861 accgctcgcc gatgtccgcg acgctcagct cgctcgcctg cagcaggcgg atggccgcat
     7921 cgcggcggca gtcgtccttg agtcgctggt agctggtctg ctcgctcatc aggcgacggc
     7981 gcaggctgga ggacgacatg ccgaggcgct cggcgatctc ctcgaagctg ggcaggccgt
     8041 cggcgaactt gttgccgagc aaggagcgga tcgccgcact ggtgctcgcg ggcgcgtggt
     8101 ccatctgcat gagctgatag gggccggtgc gcagcatgtc ctcgagcgag gcagcgtcgt
     8161 gcaccagctt gcagtcgaga aaatgcgcgg ggaactcgag gaaggaattg ccctcgacgg
     8221 cgcgcaccgg gcagcggaat atgccggcaa gtttgctggc gtatccctcg ggcagcgcgc
     8281 tcacgcccac gccgacctgt tccggctcca gcgcgcgacc gatcaaccag ccgcagaagg
     8341 catgccacac cgtgaggccg gagccgcgcc ccagcaccag cggccagatc gtgccgtgga
     8401 gagcgcgcgg aatgaagcga ttctggctgt cgagcagacg atgctggtac accagccgag
     8461 ccagatcacc gtggcgctcc agcgtcatgc ggtcgccctt gagctgcggc gatatcaggc
     8521 tctcgaactc gctcgcccgc gcgagcgcct cgccgagcgt acggcaggtg atcacgcaac
     8581 aggccatgag ccggaaggcc ttgccgccca tccccgcggc ccagggcagc gccagatcat
     8641 gggactgcag gcgaaccacg gattccgtat acagcaggcc gtagtagagg ctcggcagca
     8701 catcgctggg cgcggcgcac tgcagcgtgc gggcgtcgat gccgacggcc gcagccagcg
     8761 cggcgccgtc gagcccctgc tcgtcgagat aggcgagcag ccgccggaac ttgctcaccg
     8821 gcacgcccgt cgcgtccaac tgctgtgctc tcttcgccgc cgccaattct ctcccccgtc
     8881 cgtgaaccaa tatgcgagag caattgaacg ccaaggggct tgttttcaca agcaagtcca
     8941 ctaggctagg ccgtttggac cgaccggcgc cccgcgcccc tgctcggttt cctcgcccac
     9001 acgaccacag gagtccctcc ggatgaacag caagaccctg ccgacccgcg cgcgccgcct
     9061 gacgcccgac atggacttct tcgatggcgc tgacgtcgtc gtggtgggac tgggctcggc
     9121 cggcgccagc gccgcgatcg aggccgcgga acagggcgcc acggtgctcg tgctcgaagc
     9181 ggccagcgcc ggcggcggca ccaccgcgat ggccggcgga ctgatctaca tgggcggcgg
     9241 cacgcccacg cagaaggcct gcggcatcga ggacaccacc gaggacatgt acgagtacct
     9301 gctgatggcc agcggtccca atgccgatcc cgcgcgcgtg cggctctatg ccgacggcag
     9361 cctcgcgcac tacgactggc tgctcgcgca gggcatggag tacaagcccg agtattacgc
     9421 cagaaaacac accaacacgc cgggagacga gggcctgatc tacaccggca acgagacctg
     9481 caacgagttc cgtgcccgcg ccgcgcccgc cccgcgcggc cacaagggca aggcccaggg
     9541 cgaggacggc ggccggatgc tgatggagaa gctgatcgcc tcggccaccg cgaagggcgt
     9601 gcgtatccgc tgcgatgcgc gcgcgctgac cgcgatcgtc gacgaggccg gcgccgtcgc
     9661 gggcgtggtc gcgcgcatcg acggcgaaga gcgcaacatc cgcgcgaggc gcggtgtggt
     9721 gctctgcgcc ggcggcttca tcatgaacaa cgagatggtg cagcgccacg cgccgcacct
     9781 gctgcacgag accgtgctca acggcaatcc caatgacaac ggcatgggta tccgcatcgg
     9841 catgggggcc ggcggcgcgg tgatgaacat gaacgaggga ttcgtctgcc tgccgttcta
     9901 tccgcccgca gacctcgtcg aggccatcat gatcaacagc aagggccagc gcttcatcaa
     9961 cgaggactgc taccacgggc gcatgggcga ggcgatcctg aggaactatc ccgagaagca
    10021 ttacctcgtg atcgacagcc ggctgttcca caccatggaa cgcccgccgc tcggcggctt
    10081 caaggtggtc gccaccggtg acaccgtcga ggagctcgaa gccgagctcg cgctacccgc
    10141 cggcacgctt gccttcacgg tcgacttctt caaccgccac gcccgcaacg gcgaggatcc
    10201 cttgttccgc aagcacgccg actacctggt gccgctggac aacccgccct acgccgcctg
    10261 cgacgtgacg cccgggtcag gcgcgttcta cccggtattc acgctgggcg gactgcacgc
    10321 gcggccgacg ggcgaggtac tgaccgagga caacgtcgtg gtgccagggc tgttcaccgc
    10381 ggggcgcaac tgctgcggcc tgccgcgctc gggcgccacc tacagcagcg ggatgtcgat
    10441 cggggacgcg accttcttcg ggcgcctggc gggacgcagc gcggccgcgg cgcccgctcg
    10501 cccgcactga cccatagcgc atggtcgagc tcagcctcgt cagcgtctcg ctgctggtcg
    10561 tcaccggttt catcgccggc gtgatcaaca cgctcgccgg cggcggctcg aacctgacga
    10621 tacccgcgct gatggtgatg ggcttgcccg ccgacgtcgc caacgcgacc aaccgcgtcg
    10681 gggtcttgct gcagtcggtg gcgggcgtgc acggttttca ccgccacggc aagctcgatt
    10741 cgcacgacac ggtgccggtg ctgatcccga acctgctcgg cggcctgctc ggggcggtgt
    10801 tcgccgccgc gatcccggtg agcttcctga agccgctgct gctgggcacc atgatcggga
    10861 tgtcgctgct gatactgctg cgtcccgccg tgatcgcccc gccgcccggc acgccgccgc
    10921 tgtcggtgcg cgagcgaccg gccgcgtggg ccgcgctgtt cctcgccggg gtctacggcg
    10981 gattcgtgca ggccggtgtg ggcttcgtgc tgatcgccgc gctcgccggc acgctgcgct
    11041 acgacctggt gcgcaccaat gcgttgaaga tgctgtgcac cggtgcgttc acgctgatcg
    11101 cgctcgtggt cttcatcgtc gacgacctgg tgctgtggct gccgggtctt gtcctcggcg
    11161 tcagcacgat gttcggtgcc gcgctcggcg tgcgcatggc gctgaaggtg cggcaggaaa
    11221 cgctgaaatg gttccttttc ctgatgacgg tctgtgccag cgcggcggcg ctgtggcatt
    11281 gagacctgat ccgtgctggt tcgcggtttc gatcgaaact gctcccggac gatgaaccac
    11341 caacggtagg cgcgccgagc gccgggcctg tgccgcgagc ggacatgtgg atgacgaaat
    11401 cgccgggagc gatttcgtca acacgcgtag ccgccgcaca gggatgtgcc aggcggcggc
    11461 cgcgcgcggc acaggcccgg cgctcggcgc gccggcccgg atccctaccc ccgtgagttc
    11521 attggcaact tgccccgatc acgacaaccc cgccaccggc accagcgcgc cgtggatcgc
    11581 gcgcgcggca tcgctgccga ggaagcagat cacgttgccc aaatcctgcg ggctgaccca
    11641 cagcgaggga tcggcgtcgg gcatgtcggc gcggttgcgc ggcgtgtcga tcatgctcgg
    11701 cagcaccgcg ttcacgttga cgccgtgatg cttgagctcg gccgagaggc tctcggtcag
    11761 gcgcatcacc acgctcttgg cggcacagta ggcgctcatg tgaccctggc ccttcagcgc
    11821 gccgagcgcc cccacgttca cgatcgcccc gcgtccgcgg gcgaccatgc cgggcaccgc
    11881 tgcgcgcacg gcgttctgca tcgtggccac gttgatctgg tacatctgcg cccactcgcc
    11941 gtcggtgact tcccaggtgc gcggccccat cgcgaaaccg ccggcgaggt tgaacaaccc
    12001 gtccaccgga ccgatctgtc cgaagcacgc ggcagtcgcg ccggcatcgc cgagatcgac
    12061 gcacaggcgc agcgagccgt cggccggcgc gtcggcggga aatgcgaggt ccagcagcac
    12121 cgtgtcggcg ccgagcgcgc gggcgcattc gctgactccg cgccccagcg cgccggcggc
    12181 gccggtgatc acgatgcgtt tgcccttcag agtcatgaga ttctctccgg tggaaccgag
    12241 aaacgtgttt gttccgcgcg aatcgcgagc acgcgaatcg tcacaccttc cgcgcggcgc
    12301 ccgcccagta cgtcgtgcgc agcgccttct tgtcgggctt gccgatgggc gtgagcggga
    12361 tcgcgctcac gaagtccagc gacttcggcg cctgcaccga gcccttggcg tccttgaccc
    12421 gcgccaccag ctcctcgcgc agcgcatcgc cctgctccac gcccggcttc agcaccacca
    12481 gcgccttcac ggcctcgccc cagcgctcgt ccggcacgcc gatcaccgcg acctgcgcca
    12541 ccgccgggtg cgccgagatc acgtcctcga cctcgcgcgg gaacacgttg aaaccgccgg
    12601 tgaccaccat gtccttcttg cggtcgatga tgtagaggaa accctcgctg tcgaagcggg
    12661 caatatcacc cgtgtgcagc cagccgccgg cgaaggcctc ggcggtctgc tcgggcaggt
    12721 tgtggtagcc cttcatgacc agcggggcgc gcacgcagag ctccccgggc tgcccggggg
    12781 caacgggatt gttgtcgtcg tcgagcagcg ccaggtgcac ccacggcgcc gggcgcccgc
    12841 aggaggcgag acgctccggt ttcgacaggt cgtgctcggc cttcttcatg ttcgccagcg
    12901 ccatgggcgc ctcggactgg ccgaaatact ggtagaagac gtggccccac ttctcgatgc
    12961 cttcgcgcag ccgcgccggg ctcatcgccg aggcgccgta gaagatcgtc tccaggctcg
    13021 acagatccgc ggtcgccgcg cgcggcgaat cgagcagggt gtagatcatc accggcacca
    13081 gcatggtcgc ggtgatgcgg tgctgctcga ccatgtcgaa gaagagatcc ggcgtgaagc
    13141 cgcgcggcac gtagaacgcg ccgccgcgct gcagcaccgg gatgaagaag gccgccgccg
    13201 cgtgcgagag cggcgtgcac atcagcatgc gcagttcctc ggggaactcc cactcggcca
    13261 tctgcagcat cgtcaggtag gcgccgccac gggcggtcga catcacgccc ttgggcttgc
    13321 cggtggtgcc gccggtgtag ttgatcgagc tcacgtcgtc ggggcggatg tcgggcgcca
    13381 ggagcggcgc ggcgctgaat tgcgcggcga gcgcaaggta gtccacgccg accgtgctgg
    13441 gaccgaaagc cagcaggttc ttcaacccgg gcaggcgtgc cttgagctcg gcggcgcgct
    13501 gatcgaaggc ggacggatcg tagaccagcg tctcgatccc cgcgtcctcc agcacgtagg
    13561 cgtgatcgtc gagcgatccc agcggatgca gcggcgtgcc gcagcagccc gtgacctgca
    13621 tcgcggccag gttggtcagc acctcggggc ggttgccgga gaggatcgcc acccgcgatc
    13681 caacgccgag gcccatcgcc ttcagcgcct ggtgcatgcg gctggcggtc gcgcgcacct
    13741 cggcataact cagcacggca tcgccgatga acaggcaagg ccgctcgtcg tagcggttca
    13801 gcccgtcgat cagcatgtgc accatcagcg ggggttcgtg gatgcggtcg tggttcatca
    13861 tgcaattcct tggtggcaca gccgcgaatc atagcgtccc gcgtcgccgc gtacatgacg
    13921 atcgtgcaag ggaaccgggg ccggggaagg tttcagcggt cgtcgcgata gcgacgctct
    13981 gcgagcaagg cggcggacac gctcgcgggc atggcactag ccgcgggcat ggcccgctcc
    14041 tacttcaata cgtcgcggct gatcagctcc ttcatgatct cggaggtgcc gccgtagatg
    14101 cgctgcacgc gcgaatcggc atagaagcgc gagatcgggt attcgcgcat gtaaccgtag
    14161 ccgccgaaga gctgcaggca gccatcgacg acctggtcct ccagttccgt gcagctcagc
    14221 ttggcgacgc tggcggtcgc cgagtcgagc tcgtggcgca tcaggctggc gatgcagctg
    14281 tcgaggaaac gctcgtgcac cgtgatcgcg gcggccatct cggccagacg ataacgcgtg
    14341 ttctgctgcg cggcgagcgg ctggccgaag gcgtggcgct ccttgacgta ggcgaccgtg
    14401 atgtcgagcg caccgcgcgc gtgtgcgacc gcgccgatcg agagccccag ccgctcgcgc
    14461 ggcagttcgt tcatcagcac cgcgaagccg cggttcagtc cgcccagcag cgcctgcgga
    14521 ccgacgcgca catcgctgta gaagagttcc gaggtgtccg cggcgtgcag cccgagcttt
    14581 tccaggtttc gcccgcgcgc gaaacccggc gcgtcggcat cgaccagcag cagcgatatc
    14641 cccttcgagc ccggcgccga gagatcggtc ttcgccgcgg tgatcacgag atccgcgtgc
    14701 tggccgttgg tgatgaaagt cttggcgccg ttgatcttcc agccctcgcc atcgggctgc
    14761 gcgctggcgc ggatcgattg caggtccgat cccgccccgg gttcggtcat cgcgatggcg
    14821 cccacgcaat cgccgcagac catcttcggg aggtagcgct gcttctgctc ctcggtgccg
    14881 gcattgagca cgtagtgcgc gcagatgtcg ctgtgcaccg tgatgttgca ggcgagcgcg
    14941 aggaagcccg cgcgcgcgat ctcctgctgc accaccaccg agaagcggaa atcggcaccg
    15001 acgccaccgt actccagcgg gatgtcggtg ccgagcatgc cggcagcacc gagttcgcgc
    15061 caggtttcgc gcgggatgat gccggcgctc tcccattcct cgtaaaacgg cgccacgcgc
    15121 tccttcacga agcggcgcac gctgtcgcga aacagggcca gctccgtatc ctgttcgctc
    15181 gcgctcatcg tgaatcctcc gccgggaaag ggcgcgagct tagcatcagc gccccggtgc
    15241 gggccagccg ggcattcatc gcaacacctg tcgtcactgc ctccgccgcg gcgctggcgg
    15301 caaatgccgc cgacgcacta aacgcgacga ttccccgccg cagcacgcag taaagtcctg
    15361 gtaattccca gcagccgcac gcgtggcgta gactccgcgc gcgcccgccg ggcgggtact
    15421 gtccacgatc acgaattgcc agggtgaatg catgaatgta ccggtagaca agagcagccc
    15481 ctcgcgcccg cgccgcctcg ccgacgtcgg gcgctgggac atggaaaccg atgtcgcgat
    15541 catcggcttc ggcggctccg gagccgcggc ggccatcgag gccagcgacc agggcgcggc
    15601 ggtgacgatc ttcgacctcg ccagcgccag cggcggctcg accgctctgt ccagcgccga
    15661 gatctacatg ggcggcaacg gcggcacgcg cgtgcagcgc gcctgcgggc tggaggactc
    15721 gaccgaggac atgtaccagt acctgatggc cgccaacggt ccgctggccg atccggacaa
    15781 ggtgcgcgcc tactgcgagg ggagcctcga tcatttcaac tggctggtcg gcctcggcgt
    15841 gccgtacaag gacacgcttt acaaccagcg cgccatcatg gcgctcaccg atgactgcct
    15901 gctctatacc ggcagcgaga aggcatggcc ttacaaccgg gtcgccaagc cctgcccgcg
    15961 cggccacaac ctgcaggtcg agggcgacaa cggcggcccg ctgttcatga agatcatgac
    16021 ggagaacgtc gagaaacgcg gcgtgaaggt cgtctttgaa gcgcgcgcgc tgacgttgat
    16081 cgttgacgac gagcgccgcg tgcacggcgt ggtggtgcgc atcgaccagc aggaacgctg
    16141 cgtgcgcgcc cggcgcggcg tgatcctctg cgccggcggc ttcgcgatga accaggagat
    16201 gctgcgcaag tactcgccgc tgctgctcgc ggccagcgag ccgataggca acccgggcga
    16261 cacgggatcg ggcatccaga tgggcatggc ggtcggtgcg gccgcgatca acatgcacga
    16321 gggcttcgtg agcctgccgt attacccgcc gtcgtcgctg acctggggca tcctggtcaa
    16381 cgcccagggc cagcgcttcg tgaacgagga ctgctaccac gggcgcgtgg gctactactg
    16441 cctgcagcag ccggaccggc gcgtgtacct gatcgcgaac gtcgaggatt tcggcgacta
    16501 cgagaagacc agctatctcg gcgccaagtt cgtggcgacc gcggaggaat ccgtggcgga
    16561 gctcgagagc gagctgcaac tgcccgaggg cacgctgcag aacacgatcg aggtgttcaa
    16621 ccgcaatgcg gccgagggcg tggatcccct gttccacaag accgcggagt ggctgaagcc
    16681 cctggaactg ccgctggccg cgctcgattg cacgccgggc cacggtgcct tctacccctt
    16741 cttcacgctg ggtggcctgg ataccctgcc gagcggcgag gtactcaccg ccgagcgcac
    16801 cgtgatccag ggcctgtacg cggccgggcg caccgcatgc ggcgtgccgc gcaccgcaag
    16861 cggctacgcc agtggcatct cggtgggcga tgcgaccttc tccggccgca tggccggacg
    16921 cgccgcggcg atggcgggcg cgcacgactg aggcagcgct cggggcggcg cgacaggccg
    16981 ccccgagcgc ccggcgtcat tgctcgaact tcagcacggt cttccaccac agccgcggcg
    17041 cgagcgcgtg ccacaggtcg agcagcttgg cctcgccggg atagatggcc ttcctgccct
    17101 tctcgagccc ctgctcgatc tgctccacga tcttggccgg gtcccccagg cgcccggact
    17161 tcttcgcctc gcgcagcgcc ttggccggat cggtgtccag cgactgctgg atcaatggcg
    17221 tgttaaccgc cggcggcatc accaccgcga tacgcagcgc cgtgtgcccc agttcattgg
    17281 ccagcgtctc gccgaacacg ttcacggcgg ccttggaggc cgcataggcg ccgagctttg
    17341 gcgtcagcgc agtcgcggac acggatccga acaggatcac gtcgccgcga ttgcgcgcaa
    17401 ccatgcccgg caccacagcg cggatcatgt gcagcgtgcc gaagtaattc acctccatca
    17461 gccgccgcgc cacctccagg gtcgtgtcga tagccgcgtg actcggcatg atgccggcgg
    17521 catgggtcag gcggtcgatc ggcccgagtt cgcgctcgac gtccgcgacg cgcgcctgca
    17581 cctcctccca gtcggccacg ttgcagcgcc acgcgcggat gttcggcgat tgtgccgcgg
    17641 tttccgcgag cgcggcctcg ttcatgtcga aaatcgcgac ctgtgcgccg gccgccgcga
    17701 gtcgcagcgc cgagaccttg cccatgccgc tggcgccgcc ggtgaccagt gctaccttgc
    17761 cgtggaaagc catcgggatt ctcctgtgag tggccaagca aagcgtcgag tgtagcgagt
    17821 cacacgacaa ggccgcgccg cggccagatg acgatttgtt taggccacgt ccgatcgggg
    17881 ccacggcgaa cgcccacggg cactagaatg tcggcctgtc gtccccgggt gctttcccct
    17941 gtatgcagcg catcggcgtc ctcttcgtgt gcctgggcaa tatctgccga tcgccctcgg
    18001 cccaggccgt gctggaggat cggctcgagc ggcgcgggct cgcggcgcac tttcgcgtcg
    18061 attcctgcgg cacggccgca ttcaacgtcg gcaaggcgcc ggatccgcgc gcgatcgccg
    18121 cggccgagcg ccgcggctac cgcatcgcgc accagcgcgc gcgtcaggtc gaggacgcgg
    18181 atttcgcgcg ctttcagcac atcgtggcga tggaccgcgc caacctgcgc acgctgcagg
    18241 gctgggccgg gccggacttc gccggcgaga tccacctgct gctgcacttc gcgcccggcg
    18301 ccaccaccga ggtggcggac ccgtactacg gctcgcccga agccttcgac gcggccctgg
    18361 acgccatcga gcgaggcgtc gatggcctgc tgctggcgct ctgcgcgcgc cacggcaccg
    18421 aactcgccgc cgcggcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
    18541 gccgcggcgc cgcgaacggc gcgagtgatc ggagctgtcg cattcgctat aatcctgccc
    18601 ccgctgcgcc gcgcagcaca tgaaacaggc aggttggtgt cgccgatgac ggacaggact
    18661 ctcgaagatc tcaatgtgga aaccatcgag gagctgatca ctcccgatca gctcaaggcg
    18721 gagatgccgc tcacgcccgc ggtcagcgag acggtgcgct cggcgcgcgg gatcgtgcgc
    18781 gacatcctcg accgccagga ccaccggctg ttcctggtcg tgggcccgtg ctccatccac
    18841 gacaccggcg ccgccatgga gtacgcgcac cggttgcgcc gcctggccga ggaggtacag
    18901 gacacgctgt acctggtcat gcgcgtctat ttcgagaaac cgcgcaccac gaccggctgg
    18961 aaggggctgg tcaatgatcc gcacctgaac gactctttcc gcatcgagga aggattgcac
    19021 atcgcgcgca agctgctgat cgacatcacc ggcatcggcc tgccgacggc gaccgaggcg
    19081 ctcgatccga tctcgccgca gtacctgcag gacctgatcg cgtggtccgc catcggtgcg
    19141 cgcaccacgg aatcgcagac acaccgcgag ctggcgagcg gcctgtcgtg cgcggtcggc
    19201 ttcaagaacg gcaccgacgg cgggctcacg gtcgcggtca acgcgctgaa atcggtgaac
    19261 agtccgcacc gtttcctggg catcaacatc gagggcaagg tcgcggtgat ccacacccgc
    19321 ggcaaccgtc acgcccacgt ggtgctgcgc ggtgggacga atggcccgaa ctacgactcg
    19381 gttcatgtgg cggtgtgcga gcgcgagctc gaaaaagccg gcatcgcgcc gaacattgtc
    19441 gtggactgca gccacgcgaa ttcgaacaag gaccacgcgc tgcaggcact ggtgctcgac
    19501 aatgtcgcca accagatcct cgagggcaac cgctcgatcg tgggcgcgat gctcgagagc
    19561 aacctggaag cgggcaacca gccgattccc tcggacctgg caaagctgcg ctacggcgtg
    19621 tcggtcacgg acgcgtgcat ggactggaac agcaccgagc aggccatccg ctcgatgcac
    19681 gaaaagctgc gccgcatcct gccgcagcgc tctggcgcca tcggcagctg atgccggcgc
    19741 cgccgcgcgc gacgggctgc ccgacgggca gcgcccgtcg cgtcggtgcc ggaaaattgc
    19801 cctccctcag aacagtccct cgatctcccc gctcccgttc aggcggatgc cctcggccgc
    19861 cggacgccgc ggcagtcccg gcatcgtcat gatgtcgccg cagagcacca ccgcgaactc
    19921 ggcgccgttg gcgagcacca cgtcgcgcac cgacaccgcg tggtccgtcg gcgcgccgag
    19981 cagccccgga tcggtggaaa agctgtactg ggtcttcgcg atgcagaccg ggaagcggcc
    20041 ggcgatggcg tcgtactgcc ggaacctgtc cagcaccttc tggtccgcga ccactttccc
    20101 cgcgccgtag atcgtgcgcg cgacacagca caccttgtcc cacagcggca tgtcgtcgtc
    20161 gtagagcgtg cggaactgcg agagccccga atcggcgagc cgctgcaccg cacgcgccag
    20221 cgcctcggcg ccggcaccgc cgccgtcgcg gaaatgcgtg gactcgacgg cctcgcagcc
    20281 caggcgcgcg gcctgctcct tcagcagcgc gatctcggca tcggtgtcgg catcgaagcg
    20341 gttgatggcg atcacgaccg gcacaccgaa cagcccgatg ttgcgcacat ggcgcgcgag
    20401 gttggcgcaa cccgcgcgca gcgcctcgag attctcgcgc cccagttcgc tcttgcgctg
    20461 gccaccgtgc atcttgagcg cccggaccgt ggcgaccagc accgcggcat ccggatgcag
    20521 gccacccgtg cgcgccgcga cgtcgaagaa tttttccgcg cccaggtcgg cgccgaaacc
    20581 cgcttcggtg accacgtagt cggcgagctt cagtgcgctg cgcgtggcga tcagcgaatt
    20641 gcagccgtgc gcgatgttgc cgaaaggacc gccatggatc agcaccgggt tgttctcgag
    20701 ggtctgcacc aggttcggct gcatcgcgtc gcgcagcagc accgtcatcg cgcccgcggc
    20761 gttcacctcg cgcgcgtaca ccggcgagag gtctcggcga tagccgatga tgatgttgcc
    20821 gaggcggcgc tcgagatccg ccaggtcgcg cgcgaggcag aagatcgcca tcacctcgga
    20881 ggccggcgtg atgtcgtagc ccgcctcgcg caccagcccg ttcgccgcgc cgcccagccc
    20941 gacgcagata tcgcgcaagc tgcggtcgtt catgtccacg gtgcgccgcc agctcacgcg
    21001 gcgcgggtcg atcctcagat cgttggacca gtacaggtga ttgtcgacca tcgcggcgag
    21061 caggttgtgc gccgctgtga tggcgtggaa atcgccggtg aaatgcaggt tgatgtcctc
    21121 catcggaacg acctgcgcaa aaccgccgcc ggccgccccg cccttcatgc cgaagcaggg
    21181 accgaggctg ggttcgcgca gcgcgagcag cgtgttgcag ccgatgcggt tcatgccgtc
    21241 ggcgagaccg accgccaccg tggtcttgcc ctcgccggcc ggcgtcggcg agaccgcggt
    21301 gacgaggacc agcttgccat cggggcgccc gtcgagcgcc tcgaggaatg cgcgatcgat
    21361 cttggccttg ctgcggccat aggggatgag ggcatccgcg gggatgccgg cacgagcagc
    21421 gattgcgtcg atcggcagca tcgtcgcggc acgcgcgatc gcgatgtccg gcggcaaggg
    21481 ttgcaccatc tcctaccccc aaaaaaagga gcggcgccct ggggcgccgc gcattgcagg
    21541 accgacgatc agaacttgta gcggaccgac atcacgacca gctgcgagct gtagtccggc
    21601 cgggcccagg caaaggcaag atacctcgcg ctcgcctgcg ccggatccgc cggtgtcgca
    21661 ggacccacga cctgatcgct ccagccccag tcctccgcat cccgggtctc gtagatgtag
    21721 cgcccggaca cgtcgatcct ctcgttccag cggtacacgc ccgaaaggct gaagcgcaac
    21781 agctcgtcct gactgtccgg cgtgtcgccg aacaagggca gcgccgtacc cgccgggtag
    21841 gtgctgccga ggaactcgct cgcgtagctc gaatccgcct ccaggtacgc cacgtcggcc
    21901 gacaggctga accggtcctc cacaaccgcc caatccatgc cgagaccgaa tgcatccaca
    21961 tcgtcctcgg tatcgagccc ccagtcgatg tagggggtcg ggccgccctg gttcatctgt
    22021 cgcccgatct gctcgaactc gtagctctcg cggctggcgt acgcggagag cgccacggca
    22081 tcgctcgggc tccagctgaa gccgacgttc cagatctcgc tgtcgcgctt ttcgagtccg
    22141 aaggtgtcgt tgtcgtagtc gtcgcgcacc acatcgtacc gggcatagac ggacgccgcg
    22201 gcgcccagcg cgtagttggc ctcgatctcg tagacctcct ggtcgcggtc gagcaggaag
    22261 accggcgtac cccggcgcgg gatcccgccg ggggagatga cttccccggt ttcgtcgctc
    22321 agatccgcct cgatgacgtt gtactcgccg cgcagcttga tcgtcagcgc ctcgatggtc
    22381 gcgatgctga gttgtgccca gaccttgtcc tcggtggtcg aatccgtgaa cgaggccggc
    22441 gagtagagcc tggcgacgga gtcgtactcc agttcgtcgg tatcgcgatc cgcttcctcg
    22501 tattcgtagc cgccgcgcag cttcacgcgc ccgccgaagc gatagcccgc ctccgcgccg
    22561 agggtctggg tctgcttgtc gtagaccttg gacgtgaagc ccggcgcgct gctgttgtag
    22621 gtgtagctca gctcgttgac cggcagcgga tcgtgcctgg cgtcgcggtc cttgtaggcg
    22681 tactgcacgc gatagtcgaa acgcgaaaac ggccgccccg ccaggctcag cttgacgttg
    22741 gcccgctcca cctccccgtc gaggttggag aacgggaaca ccgggttcac cgacgggtaa
    22801 tagttggtgt ccagcaccac cgggaggaag tcctcgtcct gctcgccgcg gctccagtcc
    22861 gcgaaccagc tgagtcgcgt catcaggccc atgctgtagc caccgtccat gccgaggcgc
    22921 aggtagcggt tgtccggatc cgaggcgacc tgctcgatgc ccagcggcct gaacgtgcca
    22981 ctggcgttgt tgggcacgaa ggatccgggg actatcgcgt tctggaaggc gatcgcatcg
    23041 ttgccattct cgaactccga caggtacagc tcgaagccgt gctgcaggca gcggtcggca
    23101 tagtcgatgc gcgcccgcat ctgctggtgc tcggtgtcga gctcctccgg caccagtgcc
    23161 gttccgctga agccctggtt gccgccgaag acgtcttcgc cggacttttc ctggctctcg
    23221 aagcccaggc tcacggtcca ggcgtcaccg atgttcttga agccctcgag tccgaagatc
    23281 tggcgctcgt ggcggatgtc ctgcgtcgaa aaattcgcgg catcgtagct gccggggggc
    23341 gcggcgggta tcggaaacga cgttgtgaac ttgtaggtcg gcggcagcaa catggtgtcc
    23401 gacgcgcccc cgtagaccgt gccggcctcg ccatactcgg tcttctcgat gcgatcgccg
    23461 tacagcgaga tcccgaagtc gttctggacc ccgtagctgg cctccagggc gcgcccgttg
    23521 ccgactcccg cggagacctc ccatgcgcgc gcggtggcgt cgtcgccacc gctcaggcgc
    23581 agtgcgccca ccggaaagcc gccctcgtcc tggaaggcat ccgtgagccc cgaggtgtac
    23641 tcgccgaact tgccgttgcc gtcatcggtg tagccgccgc cgagctcgat gaagttctcg
    23701 tagaccaccc tgggcttcgc ggcgctcgta tcctcgaagc catactcgtc ctgcgcctgt
    23761 gcggcgcccg ccatggcgag gccggcaccg acaatcgcga tccacagtgt cttgcgcctg
    23821 gtgttcatgt cttgactccc ctgttggata cgcttgcccg gatcagcgac ggaaggtcac
    23881 gccggagggg tggttgcttc catgcacgtt cgtatggcag ttcgtgcagc cccggccgat
    23941 cgcccggttg ctcgaggcga cattcgcgtt cgcgtagacc gtgccggggt gacgcgtgtc
    24001 gttgtggcac tgctggcaca ggaacggcgg ccgcgccgtg agcagctggt cattcacgga
    24061 accgtgcggg ttatggcact cggagcagtc gtcctgcacg ggttcgtgtt cgaacaggaa
    24121 ggggccgcgc ttctcggcgt gacacttgta gcaggtctcg ttggtagtcg cctcgaccag
    24181 catgtgctca cccttggcgc catgcggatt gtggcaatcg gtgcaggcca ccttgccctc
    24241 gcggatcggg tgacgcgaga acttgttcat gttcgccttc tgatcctggt gacagtccac
    24301 gcagacctcg gtctgcgtca cacgattcag gaccttgtcg ttacgcgagt gcacgtcgtg
    24361 gcagtcgctg caggcaacat ccgcgcggct gtgttcgctg gtcaggaagt gcttctgcgc
    24421 ggtcttgccg tggcaattca tgcagacctc ggcgcgctcg gcggcgtctt cctcgctgtg
    24481 cccctcgagg gtctgcgcga cgcggaactt cttttcctgc ctggcgtgtt cgcctccggg
    24541 accgtgacag gactcgcagc ccttcgtcgc cgcgggcgtg cgcggatccg cggccatccc
    24601 gtggggaccg ctcatgatgg ccgtcgcatg ctcgtcgtcg tggcactcgg tgcagacctt
    24661 gacgccctcg ctgtaggtgg tcgatggcgg cagcgccgcg cgatcctcag ccgctgcagc
    24721 gccgcccgcc tggcacgcca gagaaacaac gatcacgccg aacagccttg tcagcctcag
    24781 attgcgcata tcccggactc ccctttcttg ttcgctgcaa ttgcatccgg accgtgcgtg
    24841 cggccggcga cccgggcccg cgcacgagcc gttcgcgcta ctgactcgcc gccacgatat
    24901 cggcacttcc cgaaccgatc gacagtgacc ttgccaggct gaccaggtgt tcacgccctt
    24961 cgctgccggg atcgtgcagt gcgataccca ggtgatagac cttgccggcc tcgagcgcga
    25021 tctggccgct gcctgcggcc tggagcgggc gctggatttc ggcgacccag cgcccgttct
    25081 cgatgcgcat cgacgcggcc ccgacgagct cacccatatg cagcttgtcg agcacatggc
    25141 cacgctcacc cgtgcccgag gcttcgattt ccagcacgtc gaggaactcg cccgccgcca
    25201 gggccacatc gagctccgcc tgcgggcgga cgctgtcgcc cccgccggtg gccgtgttcc
    25261 tgctgcgcga gcggggaagg tatttgccga gtctctggcc gctgtcgctg gccatcccgg
    25321 gagcgtcgtc atggcatgcg gcccagcaac cggcctgtgc ggtggatttc agcgcttcat
    25381 tgcccagcat gatcgcgacc gatggcgcct tgccatccac ggccggaccc gacatctgca
    25441 ggtgcaggct gtcgccctgc actgccgccc tgagctgcac ggtgactgca ttgcggcccg
    25501 caaaaccagt gagctgcgcc tgcgaggcgc ccatctcggc ttcctcgtcg cggtggcagt
    25561 cgtggcaagt ctcaccggcg cggaacttcg tagcgccctt gtgcgaggac gccgtgagca
    25621 ctttttccca cgacatctgt cccgggtaga acagggaaac ttcggcggca gccgtcttgc
    25681 cccagtcgac ggcccatgag gccgccggca ggaccatggc gcatacgaac gcgcaggcga
    25741 tcaggcggcg ggaacgcgga actatggggc ggggcgactg atgcggcacg acctcttctc
    25801 cttttttgtg ttcgctatgc aacagcattg aaactgtagg acattacgca ctcatgtcat
    25861 tgaccaggat catcccgcgc tcagaagtcg aagctctgct cctgctcggc cgtggctggc
    25921 gccagcgctg cgtggccggc ctcttcatgg cagtccacgc aggtcttggc acccgagccg
    25981 agcctcgcgt gctcgcgcct cgccttggga ctctgcgcct ctagatccat gcgcgccacg
    26041 tcatgacagg agcggcaggt ggccgaatcg ctggcgcgaa aacgcgccag ctccctagcc
    26101 ttcatggccg gctggtgcgc caggtatttc tcgtcggtgt cgatcacgcc acgcaaatgc
    26161 ccccagacct cgcgcgccgc ctcgatcttg cgggtcatct tgggtacgaa ttcgtgctgc
    26221 acgtggcaat cggagcagcc ggcgaccacg ccgtgcggat tcgtgtaatg gaccgtgagt
    26281 tggtgctgtt tatagggaat gccatggttg tggcaaccga gacagaactc gtcggtgctc
    26341 gtcgcctcca gcgtggtatt gaaagcaacg accccgatta tccccagcag cgcgcccgcg
    26401 gcaagcaata ccagcgtccg gccgcggccg cgaccgcgcc tggatccggg ctccctgaat
    26461 atgctcactg ccgccccctg tcgctgaaga aaatcccggc aggcaccgat ccctgtcacc
    26521 gcagcgaaca acagccgtca gggcagggtc aaggccattc ctcaggcaag gatagccgat
    26581 gcggcgacaa ataggcgcga aacgccctcc gggcacgcgc cggcccggca agcgcgggac
    26641 gaggaaaggc gatcaggtga aataggcgtt ggtgcggacc gagtgatccg tgacatcacg
    26701 cacacccttc aggtcgctca gttgctcgag cagggttttt tcgacaccgt gcttcagggt
    26761 ggcatcgacc atgccgcagc cctggcaacc gccaccgaac tgcagcaccg cgacaccgtc
    26821 ctcgagcaat tccaccagcc gcacttcgcc gccgtgggcg gccaactgcg ggttcacctc
    26881 gttataaagg atgtagttga tacgatcttc cagcgagctg tcggcgtcga ccttggccac
    26941 cttggcgttg ggagccttga tcgtgagttg cccgcccatc cggtccgcgg cgtaatcgac
    27001 cagcgcatcc gccaggaacg gcaggctgcg cgcctcgaac catgccgcaa agccgttgaa
    27061 ctcgcgccgc tcgtcatcgc tcttgatgtc ggcgtcgcgg caatacgcga tgcacgtctc
    27121 cgcccgcggg gtgccggggt cgttgatgaa tacgcgtatc cccctcacct ccggatcctg
    27181 cttggcgagc aatccgacga ggtattcctg ggccgactcg gtgatcgtga tcatgccatc
    27241 gccatccgtg aaatgctgac agaaatatat tactgattta ctcgggtatt ggcgatactc
    27301 acgtgcagga ttactgattc tcagcctgcc gctgcccccc ctttgttacc atttcctttc
    27361 tacgctccgc gggcagccta ggcacgcggc catttccgga ccgaaccgag accccaatgc
    27421 aaaccgacca gactctccag caggccatcg ccgaacgcat cctcgtgctc gatggcgcca
    27481 tgggcacgat gatccagggc tatggtctgc aggaagccga cttccgcggc acgcgctttg
    27541 ccggccaccc ggtaccgctg cgtggcaaca acgacctcct ggcgctgacg cgcccggacg
    27601 tgatccggga aatccacgcg gcctacctcg cggccggtgc cgatatcatc gagaccaaca
    27661 ccttcaattc gaccgccgtg tcgcaggccg actacgaact gcagcacatc gtgcgcgaac
    27721 tgaacgaggc cggtgcgcgc gtcgcgcgcc tcgccgccga cgagttcacc gcccgcgagc
    27781 cgaacaagcc gcgcttcgtc gccggcgtgc tcgggccgac cagccgcacc gcctcgctgt
    27841 cgcccgatgt gaacgatccg ggctttcgca acatcacgtt cgatggtctc gccgcggatt
    27901 accgggttgc catcgagggg ctgatcgccg gcggcgcgga cctgctgctg atcgagacgg
    27961 tgttcgacac cctgaacgcc aaggccgcga tcttcgcctg cgagcagttc ttcgaggagc
    28021 acggccgccg ctggccggtc atgatctcgg gcacgatcac ggatgccagc gggcgcacgc
    28081 tttccgggca gaccgccgag gcgttctggt actcggtagc gcatgcccga ccgctggcca
    28141 tcggcctcaa ctgcgccctc ggcgcggaac aactgcgccc ccacgtgctg gcactggcga
    28201 aaatcgccga caccgcggtc agcgcgcacc cgaacgccgg gcttcccgac gagttcggcg
    28261 gctacaacca ggacgcggcc acgatggctg caatcgtcgg ggagttcgcc gacagcggac
    28321 tgctgaacat cgtgggcggc tgctgcggca ctacccctgc acacatccgc gcgatcgccg
    28381 agcgcgtcgc gggcgtggcg ccacgacgca tcgggcgcgc accggcggcg ttgcgcctgg
    28441 cgggcctcga accgctggtc gtcaccgccg attcgctgtt cgtgaacatc ggcgagcgca
    28501 ccaatgtcac cggctcggcc cgctttgcac gcctgatcag ggaccaggac tacgacgccg
    28561 cgctggatgt cgcgcgccag caggtcgagg ccggcgcgca actcatcgac gtgaacatgg
    28621 acgagggcat gctggattcg cgcgccgcga tggagcgctt cctgaacctc gtggcctccg
    28681 aacccgacat cagccgcgtg ccggtgatga tcgactcctc caagtgggac gtgatcgagt
    28741 ccgggctgcg cgtggtgcag ggcaaatccg tcgtcaactc gatcagcctg aaggaaggcg
    28801 agcaggaatt cctcgcccac gcgcgcctcg cgcgccgcta cggcgccgca gttgtggtga
    28861 tggccttcga cgaacaggga caggccgaca cgctggagcg caagatcgcg atctgtacgc
    28921 gttgctacga actgctggtg cacgaggtcg gcttcgcccc cgaggacatc atcttcgacc
    28981 ccaacgtgtt cgccatcgcc accggcatcg aggagcacgc gaattacgcc gtggacttca
    29041 tcgaatgctg ccgccacatc aagcagcacc tgccgggagc gctggtttcg ggcggcatca
    29101 gcaacgtctc gttctcgttc cgcggcaacg accgcgtgcg cgaagccatc cacgcggtgt
    29161 tcctgtacca cgcgatccgc gccggcctcg acatgggtat cgtcaacgcg ggccagctgg
    29221 ccatctacga cgagatcccc gcggagctgc gcgaacgcgt cgaggacgtg gtgctgaacc
    29281 ggcgcgccga tggcacggaa aggctgttgg agatcgccgc gaaatacgcc ggcggcgccg
    29341 cgatcgcggc cgtcgaggac ctggaatggc gcggctggcc ggtcgagcgc cgcctcgagc
    29401 acgcactggt caagggcatc aacagctgga tcgaggaaga caccgaggca gcacgcgtgg
    29461 cggccgcacg cccgctcgac gtgatcgaag gccccctgat ggccggcatg aacgtggtcg
    29521 gcgacctgtt cggcgccggc aagatgttcc tgccccaggt ggtcaagagc gctcgcgtga
    29581 tgaagcaggc ggtggcctgc ctgctgccct acatgaacca ggatcgcgcc gagggcgagc
    29641 gcaccaccaa cggcacggtg ctgctggcga cggtcaaggg cgacgtgcac gacatcggca
    29701 agaacatcgt cggcgtggtg atgcaatgca acaacttcga ggtcatcgac ctcggcgtga
    29761 tggtgtcctg cgagcgcatc ctcgccgccg cgcgcgagca cgatgtcgat gtgatcggcc
    29821 tgagcggact gatcacgccc tcgctggacg aaatggtcca cgtggcgagc gaaatgcagc
    29881 gcctgggctt cgatatcccg ttgctgatcg gcggcgccac gacttcgaag gcgcacacgg
    29941 cggtgaaggt cgagcccgcc tacgccgcgg gccccacggt gtacgtgccc gacgcctcgc
    30001 gcggggtcac cgtggtgtcg cagctgctga gcgacaccca gcgcgcggaa ttcatcaccg
    30061 agcggcgcca cgaatacgcc cggattcgcg agcgcaatgc gaatcgccct gcgcgcgcgg
    30121 cactgctctc ctacagcgac gccgccgcca atgcgcccgc cctggactgg gccgcgtacc
    30181 agccaccgcg gcccgccttc accggcgtgc gcgtgttcga cgattatccg ctcacggaac
    30241 tgatcgacgt catcgactgg acgccgttct tcatctcgtg ggatctcgcc ggcaagtatc
    30301 cggcaatcct cgaggatgcg acggtgggag cggcggcgcg caacctgttc gatgatgcgc
    30361 agaagctgct gcgccggatc atcgacgaga agctgctggt cgcgcgcgca gtggtaggct
    30421 tctggcccgc ggcacggctc gggagcgacg atatcgcggt atacaccgac gagacgcgca
    30481 gcgcccgcct ggaggtactg cactgcctgc gtcaacagca ggcgcaggcc gacggtcgcc
    30541 cgaacctcag cctggccgat tacgtggccc cggcaggtgg cccgccggac tggttgggcg
    30601 cgttcgccgt cagcacgggc ttcggggccg acgagctcgc agcacgcttc caggccgagc
    30661 acgacgatta caacagcatc atgaccaagg cgctggccga ccgcctggcc gaggcactgg
    30721 cggagcacat gcaccggctg gtgcggcgcg agttgtgggg gtatgcgccc acggagagcc
    30781 tgtcgaacga ggacctgatc cgcgaacgct atcgtggcat ccggcccgcg cccggctatc
    30841 cagcctgccc cgaccatacc gaaaagcgcc ccctgttccg cctgctggac gccgagcgca
    30901 gcaccggcat cacgctgacc gagaacttcg cgatgcaccc ggcggcggcc gtcagcggct
    30961 ggtacttcag ccaccccgac tcacgctatt tcgcggtcgg gaagataggg cgcgaccagc
    31021 tcgaggatgt ggcggcccgc aaaggcatgc cggtggacga aatggagcgc tggctgcggc
    31081 cgaacctggc ctagaccggc gcgcgcaggc agcatttaca actggccgat gaggaaacag
    31141 atgagtcaac agtcaccggg aagggaaaac ggcggcggcg acgagggcag gaagtcagtg
    31201 cctctgctcg gcgtggtcaa gagcattgcc gcagccgcat tcggcgtgca gagcagccgc
    31261 aacagggaag ccgacttcac ccacggcaac taccgccact tcatcgtcgg gggcatcatc
    31321 ttcaccgtca tcttcgtgct gacggtcgcc ttcgtggtgt cgctggtact gaagaatgcc
    31381 ggcgcgtcct gagcgcggcg cgccgggcgc cggccttact gcccggagat ccagaacagc
    31441 gccgtaaaca ccgcgatcgc gaccagcagt atggcgctga tggcatcgac cttgctgtct
    31501 gcctcgcgtt cctcggcgga gatctcgatg gaattgtcgg ggttggccat tggatcaggg
    31561 tttccttgag gggatgtgga ttccgagcgc gcgattgtgg acgattggcc cgcccctctc
    31621 aagcctgcgc ggcatcctgg ccgacctccg cagggaatat cactgatgat tatttggtta
    31681 taaaaattga tttcttttat agccataatc gttctgctac tttgagcgcc gttgccccga
    31741 cgtgattcgt ggcgctccct aggcgccagt gctggcgggc aaccccactg gatcaccaag
    31801 ccctcgacca ggacatcatg taccgatacg acgatctgga ccaacgcatg ctggatgacc
    31861 gcgtgcggca attccgcggg cagaccgagc gctacctgcg cggcgaactg gctgagaacg
    31921 agttccgccc gctgcgcctg cagaatggcc tctacgtgca gcgcctcgcg ccgatgctcc
    31981 gggtcgccat cccctacggc atgctcgcca gtcgccagct gcgcaagctg gcccacattg
    32041 cccgccactt cgacaagggc tacgcgcatt tcagcacgcg ccagaacgtg cagttcaact
    32101 ggccacggct ggaggaggtt cccgacatcc tcgccgaact cgccagcgtg cagatgcacg
    32161 cgatccagac cagcggcaac tgcatccgca acaccaccgc ggaccagttc tcgggcgtgg
    32221 cgccggacga ggtcgaggac gcgcggccat actgcgagct gatccggcag tggtccacct
    32281 tccaccccga attcagcttc ctgccgcgca agttcaagat cgcggtcagc ggctcgcgcg
    32341 ccgaccgcgc cgcggcgcgc gtacacgaca tcggcctgat catcgtgcac gatgccgccg
    32401 gcaacaccgg tttcgaggtc ctggttggcg gaggtctcgg gcggaccccg atcatcggca
    32461 cgacgatccg cgagttcctg ccggtcgcgg acctgctgag ctacctggag gcgatactgc
    32521 gcgtctacaa ccagtatggc cggcgcgaca acctctacaa ggcgcgcatc aagatcctgg
    32581 tgaaggcgct gggcgcggag gaattccgcg cgcgcgtcga acgcgaatgg gaaatcaccc
    32641 gccagcgcat gccggcactc ggcgccgcgc aaatcgagca cgcacgctcg tatttcaccg
    32701 ccccgcccta ccgcccggtc gacgacgccg cggccgagtc gctactcgcc ggacagcgcg
    32761 cggcgaaccg cgctttcgac cgctggatga gccgcaacgt gtcggggcac cgcgtgcccg
    32821 gctacgccat cgtgacactg tcgctcaagc ccaccggcat cgccccgggc gatgcgacgg
    32881 accggcagat ggagctcatc gccgatcttg cggacagcta ttccttcggc gagatccgcg
    32941 tcgcccacga acaaaacctg gtgctcgccg acgtcgagaa ggagaagctg ctcgcgctgt
    33001 gggaggccgc gcgcgacgcc ggcctcgcca cgcccaatat cggcatgctg acggacatca
    33061 tctgctgccc gggcggtgat tactgctcgc tggccaacgc caagtccatc ccggtcgcgg
    33121 aggccatcca gcgtcgcttc gacgacctcg actacctgca cgacctcggg gacatccacc
    33181 tcaacatatc gggctgcatg aacgcctgcg gtcaccacca cgtggggcag atcggcattc
    33241 tcggcgtcga caagaagggc gaggagttct accaggtctc gctcggcggc gacgccggca
    33301 ccgaggccgc gctcggcacg attctcgggc cgtccttcgc ccaggaagcg attcccgcgg
    33361 tggtggcgca gctgctcgac gtatacgtgg cgcggcgcat cgacggggag actttcctcg
    33421 aaacctaccg gcgcatcggc atcgaggcat tcaaggaggc ggtttatggc gcggctcatt
    33481 aaggacggcg cggtggtcca ggatgagtgg acccgcctcg gcgcggactt cgacaccgac
    33541 gcgacgctgc ccgcgggaaa gctgatcgtg ccgctgcatc tctgggaggc gcgcggcgag
    33601 gaactgcgcg cgcggagcga gccgctcggc atctggatcg acagcagcca acacccggaa
    33661 gcagtgggtg atgccctggc gtatctgcag ctgattgcga tcaatttccc ggtattcagc
    33721 gacggccgcg ggtattccta cgcccgcctg ctgcgggaga agtacggctt cagcggtgaa
    33781 ctgcgcgcca tgggcgacgt gctgcgcgac cagctgttcc tgttgcgacg ctgcggcttc
    33841 gacagcttcg agatccgggc cgaccgcgat gccgccgacg cactcgcggg cctcggcgac
    33901 ttcgcccacg tctaccaggg cgccaccgtc gatccgggca ccccgttgct gctgcgcgga
    33961 gcgcaggaag cgaggccggt ccgcgccaac gcctgagcta tactgcccgc gagttcacac
    34021 gcgggagaat cggatgcgcg ggcccacggc ggcagtacag gggagccggg ggccgagggg
    34081 cggcgcgggg acgcattgcg gccgcgcgcg tcactgagcg gggatcgcgt ccgtggagca
    34141 gtcgcccgtc accttttcct tcttcctgat cttcaccggt gccgccgccg tggcctcgct
    34201 ggccctctac acgcgccagc cgttgctgat cgcctacatc gtgctcggtg ccctgatcgg
    34261 cccctacggc ctcgcgctga tcaccaacct cacgctgctg caggacatcg cacacgtcgg
    34321 gatcatcttc ctgctgttcc tgctgggcct ggacatgcag ccgcgcgccc tgctcaccac
    34381 gatgcgcaac tcgacgctgg tggcactcgc gagcgcgctg tgcttcgcat tgatcggtgc
    34441 gggcgtcgcg tatgcattcg gcttctcgcg caccgaggcg ctgataaccg ggctcgcgat
    34501 gatgttctcc agcaccatca tcggcatcaa gctgctgccg accacggtgc tgcatcatcg
    34561 ccacaccggc gaactgatgg tcggcctgct gctgttgcag gacatgatcg cgattctcgt
    34621 gctggtcgcg ctgctgggca gtcgcagcgg cgacttcgag ggcggcgccc tgctgctctc
    34681 tctgctcgca ctgccgctgc tgatcgcgct ggccgtcgtc ttcgtccggc tcgtgctgat
    34741 gcggctcatc cagcgtttcg accgcttcca cgagtatatc ttcctgctcg cgatcggctg
    34801 gtgcctggga ctcgccgaac tcgcggcatt gatgaagctc tcgcccgaga tcggcgcctt
    34861 cgtcgcaggc gtctcgatcg cctcgagccc gatcagccag tacatcgcca tcagcctgaa
    34921 gccgctgcgc gacttcttcc tgatcctgtt cttcttctcg ctcggcggca gcttcgacct
    34981 cggcgtgctc ggcagcatat acgtcgccgc catcgtgctc tcgctgctga tgctggcggc
    35041 caagccgctg gtgttccgct tcctgctcgg aaggctcagc gaaaccccgg cgctagcctg
    35101 ggacgtgggg ctgcggctcg ggcagaacag cgagttctcg ctgctgatcg cctatatggc
    35161 ggcgggtttc ggcatgatcg gcaaggaagc ctcgcacctg atccaggcga ccgcgatcat
    35221 caccttcgtc gcttcctcgt acatcgtcgt gctgaactgt cccaacccga tcgccatttc
    35281 ggaccgcctg cggcgcgact gaccccgtct ggcgagtgcg tggattcatg aaatattaat
    35341 atcgttcagg cttgatattt gacctatgcg gccgcaatat ccgccgccca ccccgatacc
    35401 cgcccccctt gcggaggacc aggaagaaat tgaaagccaa cggcaaaccg aactggaccc
    35461 cggcagattc tgccgaactt tatggaatca ggagctgggg cgcggggtac ttcgacctcg
    35521 acgacgacgg ttcggtaacg gtcagtgtga cgctggaagg caagcgcgtc agcgtcagca
    35581 ttcccgacat catcgccggc atgcagcagc gcgggctgca gatgccggtg ctgctgcgta
    35641 tcgagaacct gctggactcc cagatcaccc tgctcaacga gacctttgca aacgccatag
    35701 ccagcctgaa ctaccgcggc agctaccgcg gtgtgttccc gatcaaggtg aaccagcagt
    35761 gccaggtgat cgaggagatc gcccgcttcg gcgcggccta cgggcacggc ctcgaatgcg
    35821 gcagcaaggc cgaactgttg atcgcgctgg cgagtctcga ccccgacgcc agccatatcg
    35881 tctgcaacgg ctacaaggac gaggagttca tcacgctcgc tctgcagtcg ctgaagctcg
    35941 gctaccgctg catcttcgtg atcgagacgc ctgccgagct cccggtgatc ctcgagtgct
    36001 cgcgccgcat cggcgtgaag ccgatcctcg gcctgcgcgt gaagctcgcc tccaaggtcg
    36061 gcggctactg gaacgagagc agcggtgacc gcagcctgtt cggcctcacg acctcgcaga
    36121 tcgtggaact cgtggactcg ctgcgcgagc acgacatgct cgactgcctg cagctgctgc
    36181 actaccacct cggctcgcag atccccaata tccgcgacat ccgcagcggc gtgctcgagg
    36241 cctgccgcta ctatgtcgac ctggtcaagg aaggcgccgc actcggctac ctggatctcg
    36301 gcggcggcct ggccgtcgat tacgacggca cccagaccaa ctacgaatac agcaagaact
    36361 actcgctgga cgagtactgc tcggacatcg tcgagtcgat catgaccacg ctcgaccccg
    36421 agggcatcgc gcacccggtc atcgtcaccg aatccggacg cgcaacggtg gcctattcct
    36481 cggtgctgct gttcaacatc ctcgacgtta cccggttcga gtccggcacg ctgccacagc
    36541 agatcgaccc gaccgagcac gagctcatcc gcaacctcgc cgaggtgctg acgagcgtca
    36601 gcggcaagaa cctgcaggag tgccacaacg acgcggtgta ctaccgcgac gagatccgcg
    36661 acctgttcaa gcgcggccag atccgcctgc gctcgttgtc gctgtcggag aagctgttcc
    36721 tggagatcgt gcgcagcatc gttcgcgaat cgcagagcgc caagcgcctg ccgcccggac
    36781 tcgaggggct cgaggactcg ctgtcggaca tctactacgg caatttctcg gtgttccagt
    36841 cgctgcccga cgtgtgggcg atcgagcagg tgttcccggt catgccggta catcgccaca
    36901 tcgagaagcc gacacgccag gccatcatcg ccgacatcac ctgcgactcc gatggcaaga
    36961 tcgaccagtt catcagcccg cgcgggctgc agaagacgct ggcggtacat ccgctgcgcg
    37021 agggcgagcc ctactatttc ggcgtgttcc tggtcggggc gtaccaggaa acgctgggcg
    37081 acctgcacaa cctgatgggc gacaccaacg tcgtgagcgt gcgcatcaac gccgacggca
    37141 gcttcgactt cgtgcgcgag atgagtggcg acagcatcgc cgacgtgctg agctacgtcg
    37201 agtaccagcc gcagcaattg ctggagcagt tccgccgcac cgcggagcgc gcggtgcgca
    37261 acgggcgtat agagccggcg caacgccagc acctgctgga gtcgttcaac gcgagcctgc
    37321 gcggttacac ctacttcgaa gactgagagg agaagcatcc catggccaag gtaatcatcg
    37381 tgggtgcagg cggcgtgggc caggtggtca cgcacaagtg cgcgcaggtg cccgaagtct
    37441 tctccgagat cgtgctggcg agccgcaacg aggccaagtg tcgccacatc gcgagccaga
    37501 tcgcgcgccc gatcgcgacc gcgcaggtcg atgcgaacaa cgtcgccgag atggccgcgc
    37561 tgatccgccg cgaaaagccg gatctggtga tcaacgtggc actgccctac caggacctgc
    37621 cgatcatgga cgcctgcctc gagacgggcg tcgactacct cgataccgcc aactacgagc
    37681 cgccggacga ggccaggttc gagtacaagt ggcagtgggc ctaccaggag cgctttgcct
    37741 cgcgcgggat catgggcctg ctcggcagcg gattcgatcc gggcgtgacc aacgtgttca
    37801 cggcgtggct gcacaagcac gaattcgacg tgatcgacga gctcgacatc atcgactgca
    37861 acgccggcga gcacgggcag gccttcgcga ccaatttcaa cccggagatc aacatccgcg
    37921 aggtcacggc tcgcggcaaa tactgggacc agggtgcctg gcaggagacg gatccgcttt
    37981 ccgtcagccg catgttcgac ttcccggaag gcatcggacc gaagaagatc tacctgatgt
    38041 accacgagga gctcgaatcc ctcgtgaagc acttcccgaa ggtgcggcgc gcgcgcttct
    38101 ggatgacctt ctccgacaac tacctgaacc acctgaaggt gctgcagaac gtcggcatga
    38161 cgcgcatcga cccggtgatc tacaacgggc aggaaatcgt cccgctgcag ttcctgaaag
    38221 cggtgctccc cgatccgtcg agcctgggcc cgctgaccaa gggtcgcacc tgcatcggct
    38281 gcctcgcgag gggcacgaaa gacggcaagc cgaagacggt gttcgtctac aacatctgcg
    38341 accacgaggc ctgctaccgc gaggtgcagt cccaggcgat ctcctacacg accggcgtcc
    38401 cggcaatgat cggcgccaag atgatgctga cgggtcaatg gcgcggcgcg ggcgtgttca
    38461 acatggagca gttcgatccg gatcccttca tggaggcgct gaaccagcac ggcctgccgt
    38521 gggacgtgat gcgcctcgac ggcggcatgg actgagccgc cccgcatgcg tcatttccac
    38581 cgcttcgacg cgaggcgcgt cccctcgccc tgcttcgtcg tcgacgaggt cgcgctggag
    38641 gagaacctgc aactgctggc gcacgtgcag cgcgagagcg gcgcgaagat cctgctcgcg
    38701 ctgaaggcct tctcgatgtt cagcatggcg ccgctggtgc ggcgctacct cgccggcacc
    38761 tgcgccagcg gcctgcacga agcgcggctc ggacgcgagg aattcggcgg cgaagtccac
    38821 accttctgcg cggcgttcac cgagcccgac ctgcgggagg tgctcgcgct gtcggatcac
    38881 gtggtgttca actccttcgg gcagctcggg cgcttccgcc cgctgctgga ggcggcgctc
    38941 gccgcgcgcc ccggcctgcg gatcggcatg cgcgtgaacc ccgagcattc cgaaggcgca
    39001 gtgccgctgt acgatccctg cgcgccctgc tcgcgcctcg gcgtgccgtt gtcgcaaatg
    39061 cgcgccgatc agctcgagct gctgagcggc ctgcatttcc atacgctgtg cgaacaggat
    39121 cttccgccgc tcgcgcgtac ggtgcaggcc ttcgagcaga agttcggcga gttcatcccg
    39181 cgcatgaaat ggatcaactt cggcggtggc caccacatca cgcgtcccgg ctaccaggtc
    39241 gacgaactga tccggctgct ggtcgatttc cgcgaacgcc acggggtgca ggtctacctg
    39301 gagccgggcg aggccatcgc ctaccagtgc ggcgtgatgg tgagcgaggt gctcgacctg
    39361 acctggaacc ggatgccgca ggcgatcctc gatacctccg cgacctgcca catgccggac
    39421 gtcatcgaga tgccctaccg cgcgatgatc acgggtgccg gcgagcccgg cgagcatccc
    39481 cacacctacc gtctcggtgg ccagacctgc ctcgcgggcg acgtgatcgg cgactacgcc
    39541 tttcccgagc cactgtcgct cggtcagcgc ctggtgttcg aggacatggc ctattacacg
    39601 atggtgaaga ccaccacgtt caacggcatc aacctgccct cgatcgcgat ctggaactcg
    39661 cacagtgacg aagtgcgcgt ggtgcgccgc ttcggctacg aggacttccg ctcgcgcctc
    39721 tcttaagaca gcgcgcgccg atcacggtac acaccgagcc tgcggggggc ggtttgccct
    39781 gcgaggaccg tctgtcgcct ggatggcgac agtcgagcct acatggacgt atgcgctcgc
    39841 cacatccctg tggctcgccc tccgggccgc tttcggcgca aatcggcagt cctgccgatt
    39901 tgtcacggcg agtccgaaca gggcaaaccg cgcaccgcag gcgtgctacg tagtgctcgg
    39961 tccgcgagtc acgggaaggc tttcccgctc acgaaggatc gcgtttcgcc agcagacagg
    40021 cgaaaaaaac ccggcgcaag gccgggtttt tcgttcaccg cgcaggcgat cagagcgccg
    40081 cagccaactc cggcaccgcc tggaacaggt ccgcaaccag cccgtagtcg gccacctgga
    40141 agatcggcgc gtcctcgtcc ttgttgatcg ccacgatgac cttcgagtcc ttcatgccgg
    40201 cgagatgctg gatcgcgccg gagatgccga cggcgatgta gagttccggg gcaacgacct
    40261 tgcccgtctg accgacctgg tagtcgttcg
//
LOCUS       DAORUQ010000007        39133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_627_length_39133_cov_6.583807, whole genome shotgun sequence.
ACCESSION   DAORUQ010000007 DAORUQ010000000
VERSION     DAORUQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 39133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39133
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_39133_cov_6.583807"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..338)
                     /locus_tag="PLW13_01325"
     tRNA            complement(263..338)
                     /locus_tag="PLW13_01325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Thr,seq:tgt)
     gene            complement(380..1162)
                     /locus_tag="PLW13_01330"
     CDS             complement(380..1162)
                     /locus_tag="PLW13_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69040.1"
                     /translation="MAIAAPGEAGDATAALAPAGDAIATPGSVPAGSAAADPSAAPGV
                     AGQPDARELPDASPPAGEHRDARADDPAAAGAAQETDAVAAPEQHCVRIGPLRDGATG
                     ERMRQRAAAWLPAARVEEGEAPDRSTFWVHVPPRPSDDEARAVVSALTARGVESFVIR
                     DEPVLLHGVSLGVFRDAQSAQQQMERMQGIGYPVAIHEKPRTRRAFFLLGALGPDAGA
                     AVPPELVEAVAATTSGAGVAPVGCDALADGLTDGIAPATGSD"
     gene            complement(1339..2061)
                     /locus_tag="PLW13_01335"
     CDS             complement(1339..2061)
                     /locus_tag="PLW13_01335"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQY69041.1"
                     /translation="MILELDIGNTRCKWRLAEAPARAGACAVDELAATLGSLPERAAI
                     ARVRAGCVRGPQVEAATREAVQEVLGLETAFARSQAVAAGVRNGYAEPQRLGVDRWLA
                     MLAGFAEIGGAVCVLDCGSAITADLVDAGGEHRGGYIAPGLTMMRASLLAATDRVRFE
                     ATAAATGVAPGRDTAEAVGGGTLLAAAGFLRAARERFAQACPGAVVLLTGGDAPAIAA
                     CLDFPANIRPQLVLDGLSLALP"
     gene            complement(2058..3050)
                     /gene="birA"
                     /locus_tag="PLW13_01340"
     CDS             complement(2058..3050)
                     /gene="birA"
                     /locus_tag="PLW13_01340"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF008847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HQY69042.1"
                     /translation="MADLNEETALLALLADGEFRSGEWLAGRLGISRAGIWKRVGKLA
                     ERGIALERVSGRGYRVPGGIELLDRECIAAALPGRVRQQLSRLELRRVVDSTNALVLE
                     GLRAGTLGHGAVVFAEQQTSGRGRRGRSWVSPFGRNIYCSFAWRFDGGVAALEGLSLV
                     IGMGAVRALTRLGARDVMLKWPNDLVAGAAKLGGILIEIDGELAGPVTAVIGVGINVS
                     MPPGAATAIGQSWTDLETVCGARVGRNALAAALLESCLDILGDFARHGFGHFATEWER
                     VDALRGLEVSVSGGSGPSVQGMANGIDATGALRLMTSEGEQLLSGGEISVRRTG"
     gene            complement(3094..3981)
                     /locus_tag="PLW13_01345"
     CDS             complement(3094..3981)
                     /locus_tag="PLW13_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HQY69043.1"
                     /translation="MSSRLLVACIQHACVDWRDANLATTAALVREAAAGGARLVLLQE
                     LHTGPYFCQVEDVACFDRAEAIPGPSCEFLGSLARELGIVLVASLFERRAPGLYHNTA
                     VVFERDGSIAGRYRKMHIPDDPAYYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWYP
                     EAARLMALAGAELLLYPTAIGWDPRDTPGEQARQREAWITVQRAHAVANGVPVLVANR
                     VGHEPDPSAQGPGIRFWGHSFVAGPQGEFIARAGDSEPQVLLAELDSRRSEDVRRIWP
                     FLRDRRIDAYQDLVRRYRD"
     gene            complement(4062..5120)
                     /locus_tag="PLW13_01350"
     CDS             complement(4062..5120)
                     /locus_tag="PLW13_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQY69044.1"
                     /translation="MQTAAPPARRLPAEWEPQDGIMLTWPHEHSDWLPWLAQVEPVYV
                     AIAHAVSARERVLVCVRDAAHRDHVAALLAAGGVPRGRVALRVVPSNDSWARDHGPIT
                     IIDGGRPRLLDFVFNGWGGKYEAGLDDRITAELARQGAFGDTPREPVEFVLEGGGIES
                     DGAGTLLTTSACLLSPGRNPAFSRAAIEQRLGELFGLQRVLWLEHGALEGDDTDSHID
                     TLARFCDERTIAYVRCSERADPHFAELDAMERELRALRDARGEPYRLVPLPWPGAKHG
                     RDGRRLPATYANFLVINGAVLAPTYRDPADAGALAQLRFCFPDREIIAIDCLPLIQQY
                     GSLHCITMQLPAGTLAPV"
     gene            5287..7044
                     /locus_tag="PLW13_01355"
     CDS             5287..7044
                     /locus_tag="PLW13_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HQY69045.1"
                     /translation="MPITRNQRNHRRRGALALLCAAALLCALALRPAAGLAEPLKLTV
                     RGLDNAAQQNLRAHLGDVDVALAGQEARLQRVLTRAVRDALQPLGYYEAQFSVARDGA
                     RLVIDVEPGPRVRFAAPEIAVDEPAASLPAIRALLRSGALAAGRPLAHADYDRFRDEL
                     LRGCLRLGFFDAKYRLSELRIDLAARTATARLDLACGARYAFGTLEISGSRIDHELLA
                     SLARFRAGEPFDNELVSRFEQRLRDTGFFREVIVRVTPGADARADVTALTEDIVTSRY
                     EVGLGFSTDSALRLRFNRDSPLLNDNGHSLRIESEITESRQAVEALYRIPHRDPLDDI
                     LELTAGMQDQSVQDTDSLTATFGVRHAIKVFGDWSFGYGASVEMERYTIGRASEKRTA
                     YLMPAASISRTRIDPGVDPRSGSAWFSAIDTSDRALGSPADFLRWRGSGKWLAGLPDD
                     NTTVLARLELGAILTDGFENLPPLLRFSAGGDNSIRGYDFESIGPRDDNGKLTGSRYL
                     AVGSIEVSRRVLPQWRVAAFVDGGSAFNDSNAAFYQSAGLGLRWLSPVGQIRVDLAVP
                     VRDGENSGFKVHISMGPPL"
     gene            7041..10478
                     /locus_tag="PLW13_01360"
     CDS             7041..10478
                     /locus_tag="PLW13_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQY69046.1"
                     /translation="MSARGLSLALLRVLLWGLTAALVLALGLGATARGTALLARTLSL
                     VEPRLVIAHRDGNLLEAGFERIAWHDAALALELRDVAWTLTPRCLLTDVLCFEQLAIA
                     TLDIDIGPASGRSSPLALAPVMLPLQLSVAHGTLATLNVRRGAAPLLTLQDIALGGQM
                     HGSRIELQRLEARMGELRASLAGSVELHAQLPLEATGRLRWGAAHHADIRAGGDLARL
                     VFSARSGGPYALDVEGRIDALARPLALTLTARSRETLLLRPEQPGLAALHEATLTLEG
                     TLAALRATLAARLESEALGANAVTAAATWTPGRIDVEAFALQGEAGQLDASGHLDTGA
                     GRHWVVQARAKEFCPTGWTPALRCALSGTATLEGAFGTPLALAVESALEGTVNDHPAR
                     IAGRVAALPDGRWQVDGLRLASGANTLALEGSVGEQIALEATLEVANLADTIATATGS
                     GGGSLRVRGRRTDPQLQGTLRLRDATWNGIEAGSLVLEADWRGLAQARNSVRLALDGA
                     RWRDFAGVGLEASLTGSGAAHRIALEARAADAALRLDCAGALAVAQGWRGECRRLDVA
                     PAVAQAPWRGDRPLRLAWQRKDGEVRIEPFCLRRDAAALCSRRTAVIGAGRLAGIAID
                     GSDIPFAWLRPWLPAGLELGGNFDLRVEADKGVERNPRIEARASTGATTLTVLAAGEA
                     LDIPLGGASASLRTAGAGIALDWQLLLGGDARFTGEALLQQDSGALSGTMALRGLDLA
                     PFAKQAPGVVAAAGTLGGDVRLGGSLREPRASGVLSLRDGMLSHEKLPHPIEDIVLDV
                     EFSGAEARLAGRFGSVAGAGTLEGTARFAGAGAGWSADLGLESAGLRLEPMRGSTLTV
                     VPDLRVQLGPRHAGISGQLFIPQADIRLDRLPASAISVSPDTVIVGEEPAGAPFDYSA
                     DLEVRLGDNVRLRGRGVDARLEGRIEVLRDIGDRELRGQGEIEIADGRFTAYGQRLEV
                     TEGVVRFRGPLDRPQLRLTAVRRIDDENIQVGVRVRGDLRDPKLSTFSRPAMEESRAM
                     HYLLTGRPPSAGGNSDLVVASMLMQLGVAGASGVTSNVLRGFGIQDFQLATREVEGGT
                     EVHLSGYLRPDLYLRYGVSTFDKVNTFRLRYRLRRSVYVEAVSGIENAVDFLYSFER"
     gene            10524..11609
                     /gene="pdhA"
                     /locus_tag="PLW13_01365"
     CDS             10524..11609
                     /gene="pdhA"
                     /locus_tag="PLW13_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQY69047.1"
                     /translation="MSISARFEVAFSRFLAPEGNVEQELPPFARERDAVLRLYRAMVL
                     TRSFDTRAVALQRTGRLGTYASSLGQEAVAVGAGDAMQAGDVLVPSFREHGAQWLRGV
                     TLPEILQFWGGDERGSCFTAQPRDFPVSITVGAHATHAVGVAHAMRYRREANAVVCVF
                     GDGATSKGDVYEAMNIAGAWRSPVVFVVNNNQWAISMPRAAQSAAATLAQKAIAAGIG
                     GEQVDGNDVIAVRHAVAAALQRARAGGGAHLIECLSYRLCDHTTADDASRYRDDAEVS
                     AHWKEDPLARLRTHLGAAWNWSREEEEALQHECARAVEEAAAAWLATAPQPPRAMFDH
                     LHARLPLALEPQLATLQRHDGGTKAHG"
     gene            11602..12582
                     /locus_tag="PLW13_01370"
     CDS             11602..12582
                     /locus_tag="PLW13_01370"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQY69048.1"
                     /translation="MAELTLVEAVNRALARAMQDDAAVIVLGEDVGRDGGVFRATEGL
                     VERFGAERVLDTPVAEAAIAGIAIGMAAHGLRPVAEIQFDGFMYSTLDQMVNHASRLR
                     NRTRGRLCCPMVLRAPFGGGIHAPEHHSESPEAMFAHIPGLRVVIPSSPARAYGLLLA
                     AIRDPDPVVFLEPKRSYRALREEVADDGVALALDTCFTLREGSDLTLISWGAMLGETL
                     ATADALAAQGLAAEVIDVATLKPLDADTIAASVARTGRAVIVHEAALSGGFGAEIAAR
                     LAERCLFELRAPVLRVAGYDTVMPLAKLEQHYMPSRERILEAAHEVMRYP"
     gene            12579..13697
                     /locus_tag="PLW13_01375"
     CDS             12579..13697
                     /locus_tag="PLW13_01375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQY69049.1"
                     /translation="MSDTEFRLPDLGEGLHDAEIVQWHVAPGDHVVADQPLVAVETDK
                     AVVEIPAPHRGHIATLGAAQGERIAVGAVLVTFIATAHADTGAVVGELPLARAPVAAT
                     PARPAGSIQHLRASPAARRLAHELRCELESITGSGPDGVISVADVQRAAQVASAPAAA
                     GWESLHGARRAMAERMSAAHRAVVPASVTLHARIRAWHGRDRPLLRLIRALLHACAVE
                     PALNCWFDAGREAVLRHDTVNLGLAVNTAEGLFVPVIAAAERLDDAALLATVQALREA
                     VASRSIAPAQLREATISLSNFGTIGGETAQMVVAPPQVAILGAGRIAPGVVARDGLPA
                     VEPVLPLSLTFDHRAVTGGEAARFLEAVRAHLEQETCA"
     gene            13694..14599
                     /locus_tag="PLW13_01380"
     CDS             13694..14599
                     /locus_tag="PLW13_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQY69050.1"
                     /translation="MTPDSPVIIELAINGVTQPARNPHVPLTVEAIAADALDCVRAGA
                     AIVHSHAPLSDFNLPAEQAAEAYLASYRAVLAQRPDALLYPTIGGGATMTERLRHIEI
                     LAAAGAIRIGLLDPGCTLLGWAEADGSPSPHSFAYVNTVTDMEAALEQGARLGLGLSI
                     AIYEPGFLRNALAYWRLGRIPRGSMIKFYFGGEAGYFGGGSGVSFGLPPTAKALDAYL
                     EMMELAACPLPWSVAVMGGDLFATPLARLALERGGHLHTGLEDHLGAGQPNNLELTQR
                     AVALCRAVGRPVADPAEAARILGLP"
     gene            complement(14615..16303)
                     /locus_tag="PLW13_01385"
     CDS             complement(14615..16303)
                     /locus_tag="PLW13_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69051.1"
                     /translation="MNLRPSVLAVAASLAVLAASVPASASPASPAAVQNSPAIPPDDA
                     VRGRVAAHWFGRQWPMNFLAGFRREQVAADFRQLVDDGFDTVVLLVSWGDFQPVYAPC
                     CTYDERAFERLRFLLDRADEAGLKVMLRLGYGWSFHPQAGSVLERQQRLLNDAQAREA
                     FFGFLGRVSQEVEGREGVVMAFMSWEDLWLRRVDQAAAGTWSEYLSTLSPGPAHPPAL
                     PDAARDPVLFNAYWDWLVIAKLLEPAMALFPLLSFEARVDDEPRFEPGPDGQPVVAGW
                     IGHDGMMRIAPGYPSTIYWAPFWGSDNRGEQLPATRSLALLDALLVESAATSGRRLFI
                     DQFNFVDNTVGHGNNAVIRPEEVAAFLHAAVCVLKRREVLGYGLWTVRDYAESPLHNP
                     AFGYGLEGWRLETATGVPEQALEELASGDFQLRLLAGDRLSQQVTLRQRGRLPGAGDD
                     LPDRVCVEAEVRAPGALAVRAGSEPVVLEFDGSGLQRRCAAIAAQPDAAGLTFALELR
                     HGELALRELMIFDHVQYGGIYDLEGQPGPLLEPVRRMNRDFRARTRRCDLPDVS"
     gene            complement(16376..17236)
                     /locus_tag="PLW13_01390"
     CDS             complement(16376..17236)
                     /locus_tag="PLW13_01390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HQY69052.1"
                     /translation="MSRRRELSRRMEALADIAGIMSAMKGLALMETRILQEFLGSQRR
                     MVAGIEAAAGDFLAWHAGAGGAPAAHGGGLCVLVGSEQGFCGDFNEALLSASQRLLGS
                     RGAPPRRVVVGQRLAGRCGEREDVDLVLPGAIVADEVPAVLLRLTRELATLLARAAPG
                     ASSLSVVYHCDATREVRLRRLLPLGEPPALARGHSHAAHLTLAPADFLSGLTRHYLHA
                     VLNEVLYSSLMAENRQRQAHMDRALQRLEEETGKLRLDCNRRRQEEITEEIEVILLSA
                     QMMGLAGQPQ"
     gene            complement(17220..18713)
                     /locus_tag="PLW13_01395"
     CDS             complement(17220..18713)
                     /locus_tag="PLW13_01395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQY69053.1"
                     /translation="MAAERAPPAAGAERRATPAAYRPQLRVSEMGRVVAIGDGIVWVE
                     GLPSAAMDEILRFGDGSEALVFHLGSRRIGAILLSQKDGLVAGASAHLTGRRLDIGVG
                     DDFLGRVVDPLGRPLDGRPAPRPHRRRALEAASPPIIARDFVARPLLSGNRMVDTMIP
                     IGRGQRQLIIGDAGTGKSSLALDAVIAQKGGDVLCVYVLISQKRSAVVATVETLRRAG
                     VLDISCVVVAEATTTPGLKFLAPFAGCTLAEEWMWGGRDVLVVYDDLTTHARAYRELS
                     LLLRRPPGREAYPGDVFYLHSRLLERSTCLAAHCGGGSMTALPIVETEQGEIAAYIPT
                     NLISITDGQVYLDPALYSRGVLPAIDITRSVSRIGGKAQLPAIKAEAGRMKLDFLQFL
                     ELEVFTRFGSRLEAGVEAKIRRGRLLRELLKQERLAPLGPEQQLAWLVAYNEGFFDAC
                     AGPQVQRRLAWLLARAASAGPGLSAGREAWCACIEAWGAEAGDEPPA"
     gene            complement(18703..19449)
                     /locus_tag="PLW13_01400"
     CDS             complement(18703..19449)
                     /locus_tag="PLW13_01400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HQY69054.1"
                     /translation="MELDWTTFVLEVINFLVLVWILQRYLYRPVLNILAARKTGIERV
                     LAEAKEAEERASALKGQFESRLADWEQEKAAARASLETALALERERQMEALAEALAAE
                     RERTAAQEAHRQEALRRELAAEAGAQARRFARALLGRVAGRETEARLVELFVEELGAL
                     PEERLGALRTGQNGHARGVFATAFPLPQALRTRLAAAVEARLGMRGDFDFVEDSTLLA
                     GVRLSLGAWQLDFSVAGELGAFAESGGLGG"
     gene            complement(19462..19728)
                     /locus_tag="PLW13_01405"
     CDS             complement(19462..19728)
                     /locus_tag="PLW13_01405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937273.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; subunit C is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit C"
                     /protein_id="HQY69055.1"
                     /translation="MSDIHWFALVSTAVAAIAIALGTMFPAMAMGRAIAQALEALARQ
                     PEAEKSITRTLFIGLAMIESLAIYVLVIVLVIIFRNPLLEYLLQ"
     gene            complement(19725..20435)
                     /locus_tag="PLW13_01410"
     CDS             complement(19725..20435)
                     /locus_tag="PLW13_01410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQY69056.1"
                     /translation="MESVAVVFSLGGFGITTTVVTTWGLLLLFWIVCRLATRRLCVER
                     PGLLQTALEGVVQAIESAIDSVLPGRAATLLPFVGTIWLFLVVANLTGLVPELDSPTG
                     DLSATAALAFLVFLSTHWYGIRSVGLKDYARHYLSPSPILLPFHLLGELSRTLALAVR
                     LFGNIMSLEMAALMVLLLAGFLVPVPVLMLHIVEALVQAYIFGTLALIYIAGGMQSRE
                     VAQGGAAADSNDAGGGTT"
     gene            complement(20435..20707)
                     /locus_tag="PLW13_01415"
     CDS             complement(20435..20707)
                     /locus_tag="PLW13_01415"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQY69057.1"
                     /translation="MSDEQWRRGVERDSERMRRAERGSRSLLAQSVFLGSLSVLFVAP
                     LLAGAYLGRWLDSLGEDYTVRWTLNCILLGLVLGATNVYVFIRKYW"
     gene            complement(20704..21108)
                     /locus_tag="PLW13_01420"
     CDS             complement(20704..21108)
                     /locus_tag="PLW13_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQY69058.1"
                     /translation="MSATITLCLQSAVHSEDIAGVTSFVGTDASGSFGILPGCERFMT
                     ILGYGLSRYRDADGRWCHLACPGAVLYFAADRLTVNTRRYLRDHDYDRISGLLSGQLA
                     REEEELRSLRQNVQHLEQQLLRRLRQLDRWQA"
     gene            complement(21105..22517)
                     /gene="atpD"
                     /locus_tag="PLW13_01425"
     CDS             complement(21105..22517)
                     /gene="atpD"
                     /locus_tag="PLW13_01425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQY69059.1"
                     /translation="MDTPFVSAAPPADELRAPEPLGVIEEVHGPVIDILCTRLPPLHQ
                     ALYVHADGERYPLEVHRHLDESRARAVALHGTSGLRRHLPVFDSGGPLAVPVTPDCLG
                     RLLDLFGNPLDGAAPLAASGTRPIVAAPGPLDEAIGVGEILPTGIKVIDLLCPFVRGG
                     KTGLFGGAGVGKTVLVMEFMHAIVHLHRGVSVFAGVGERIREGHELWHDMQDAGVMPR
                     TLMVFGQMDESPGVRFRIGLSALTYAEYLRDHVARDVLFLMDNAFRFIQAGSELSGLL
                     GRMPATVGYQPTLLSEVAELQERISSTSRGSITAVEAVYVPADDMTDPAVGAILAHLD
                     TTVILARNQAAKGIYPAVDPLASGSRLMDRVTLGERHYRVAQGVREHLARYRELEDII
                     AMLGLEALSETDRRIVLRARLLERYLTQPFFVVSEHTGIAGASVPLAVTLGDCEDFMR
                     GRYDAMPEERCYMRGAMEAP"
     gene            complement(22521..23780)
                     /locus_tag="PLW13_01430"
     CDS             complement(22521..23780)
                     /locus_tag="PLW13_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HQY69060.1"
                     /translation="MTGNELMSGVTLDSNSSLIAQKLAQLNRQVAAKVTIGDDFALLC
                     AELERLAGLADVLIVNGGLGPTSDDLTAEALARVAGVPLQEQPAALAHLEAWCGRRGL
                     ALNAANRKQALLPAGATIVANAVGSAVGFRLRHRGCEIVCTPGVPSELRQMLDDEIVP
                     WLAAEFPSSERVVITRMQLFGIGESSLQQRITEEFPDWPAHIELGFRAGAPTLELKVS
                     SFRSADEADRVACEQRLQALFAEHIVGTGDTSLQRRVVELAAAAGKRLATAESCTGGL
                     IAALLTEIPGASQVFDAGFVTYSNRMKESVLGVAGASLASHGAVSEAVVRAMAEGALA
                     RSGADYAVAVSGIAGPDGGTPDKPVGTVWIAWGSERRVQARELWFPVARKLFQTMVAA
                     TALDLLRRELLGIDTEPRYFRERVRRR"
     gene            complement(23886..25076)
                     /locus_tag="PLW13_01435"
     CDS             complement(23886..25076)
                     /locus_tag="PLW13_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY69061.1"
                     /translation="MQQGVPRTVVALGFVSLCMDTSSELIHSLLPVFLVAGLGASPLV
                     LGLIEGAAEGVASVMRVFSGALSDRSGRRKPLILCGYALAALSKPLFPLAGSAATVFA
                     ARAIDRLGKGIRGAPRDALVADVTPQASRGAAYGLRQALDTVGAVLGPALAIALMYLL
                     ANDVRAVFWVATVPALLAVAVLVVFVREPAVPRPRAATQRFTFAWLRPGAVPPGCRAV
                     IALAVLLALARLAEAFVLLRASELGLATALVPLVLIVMNVAYALSAWPAGACSDRLGR
                     KPVLVCGLGLLLLADLLLARAADPWTALLGAMVWGLHLGATQGLLAALLADRAPADAR
                     GAAFGWLHLGTGLATVLAGVLGGWLWTRFGAGAAYAAGAACAALALVLLGALPGREAA
                     LAPR"
     gene            25241..26083
                     /gene="rlmJ"
                     /locus_tag="PLW13_01440"
     CDS             25241..26083
                     /gene="rlmJ"
                     /locus_tag="PLW13_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HQY69062.1"
                     /translation="MLGYRHAFHAGNHADVLKHVVLLQLLDYLAAKDKPFWYIDTHAG
                     AGRYTLTQGYAAEQREYVDGIEKLWNAGVLPEALARYVELVRTLNPHHRLKYYPGSPW
                     IARARMRPPDRLWLHELHPADYALLEQEFAAGPVPTRVFSEDGYAGLKALLPPEPRRA
                     LVLIDPSYEVREDYARLVEALKQALRRFATGVYMVWYPMIPRRESRELPARLAALAPE
                     RWLRAELVVRAADAAHGLYGSGVFVVNPPFTLRAALAESLPRLTMLLGLDRHATFTLE
                     GSAD"
     gene            26174..27139
                     /locus_tag="PLW13_01445"
     CDS             26174..27139
                     /locus_tag="PLW13_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69063.1"
                     /translation="MKIQGSQVELRAAHFEKREHRIERQLQVSITRAPPPPAADADAA
                     APAASATSALDAEVRYFQLLIEMLSGRAVRIATIASDASATGEPADGAPAASPAPSLQ
                     IAVSQRTSEHYSEAEASAFAAEATVQTADGREMRLELSLLMQRRFESTTSVTVSSGER
                     KDPLLLNLAGNAPELGAVKHAFDLDADGTQEMVAMATGRSAFVALDRDGDGRINDGRE
                     LFGALTGDGFAELARHDDDGNGFIDAGDAVYARLLAFGKDARGADVLTPLSALGVGAL
                     YLGSVATPFSVNTAANETLAVVRSTGLWIGEDLSAGTLQQLDLVV"
     gene            complement(27152..27790)
                     /gene="pdxH"
                     /locus_tag="PLW13_01450"
     CDS             complement(27152..27790)
                     /gene="pdxH"
                     /locus_tag="PLW13_01450"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQY69064.1"
                     /translation="MDLEDFRREYLAPGLHRKDLDASPFRQFESWLKHAIDAGLRDPT
                     AMAVATVDARGRPSQRLVLLKHCDDAGFVFYTNLESRKAREIAGNPRVALLFPWADME
                     RQVRIEGTVERMGAAQALKYFLLRPRASQLAAWASAQSRPLSSRALLEDEFARLKQKF
                     GEGRIPLPSFWGGFRVVPDAFEFWQGRPNRLHDRFSYTLADDGQWRIERLAP"
     gene            complement(27852..28529)
                     /locus_tag="PLW13_01455"
     CDS             complement(27852..28529)
                     /locus_tag="PLW13_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HQY69065.1"
                     /translation="MPAGVLALIVALAMLLLLPFLLADAMVSALLGLGIAPRVATLLV
                     IGIFVGSLFNVPVWRRAGAAVLEYRPSDFFGLGRLAPRLVQPGAPQVIALNVGGCLIP
                     SLIVVYELARLLDAGGGAPLHAAVAIALNVAACYLMARPVAGLGILLPALVPGCLAAL
                     VAMLLAGEHAAPVAFCAGVLGPLIGADLLHLRELGRRGGGLMSIGGAGTFDGIVISGF
                     LATMLST"
     gene            28672..28851
                     /locus_tag="PLW13_01460"
     CDS             28672..28851
                     /locus_tag="PLW13_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69066.1"
                     /translation="MQTDTGIDVKNLSDRRLWEILRLAETDGQRAATALIAHARDELS
                     VRRQHTETRRFRAPH"
     gene            28925..29473
                     /locus_tag="PLW13_01465"
     CDS             28925..29473
                     /locus_tag="PLW13_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQY69067.1"
                     /translation="MERAGDTALLQNIHRLDLLAFGWCHQRNAEAMIFVAARRISRSA
                     DGWLYLLLPLLFLASDTIDAVGFIALGCQAFALERSAYLLMKNTCRRRRPPDILPGFR
                     SLVIASDRFSFPSGHTSAAFLFAGLCTQEFGLWAAVLLYPWACCVGASRVFLGVHFPS
                     DTVAGALLGSALLLGARTLFTA"
     gene            29470..30570
                     /locus_tag="PLW13_01470"
     CDS             29470..30570
                     /locus_tag="PLW13_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQY69068.1"
                     /translation="MKIFYGVQGTGNGHLTRARAMARALARYPQVQVDWLFTGRERGD
                     FFDMQCFGAWQWRRGLSFATRAGRVRPLATLRQLSPGEFLRDVRELDLEGYDRVVSDF
                     EPVTAWAARRQRRDCIGLGHQYALVPGVPRSRRHLVGSVVLRACAPVGTRIGLHWHHF
                     GHPLLPPIVDDDARPADVPARASAREALVYLPFEDPERVIALLRAIPGWRFSMFGPGL
                     PREQRGNVTTHPVGRATFVAALERASHIVCNCGFELISEALCHGKRILAKPLAGQMEQ
                     VSNALALQALGYARIAQRLDPQLIGDFLALGTTAPRSDWPDVAAAIATWLVEGGEPLE
                     ALSERLWRECAIAEPRAGSDASDRPAALRVPA"
     gene            complement(30576..30986)
                     /locus_tag="PLW13_01475"
     CDS             complement(30576..30986)
                     /locus_tag="PLW13_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY69069.1"
                     /translation="MSNDTLDLACRYAALIDDRRLDELHTIMLPSIRITGPGYVMDTL
                     PEVERGMELLRQYDRTFHMVGNQLGAWGDDGVWSGETYCIASHVYQRDGAEWKLDMAI
                     RYQDRIVRHDGGLRFASRELRLAWVQDLPLTMAR"
     gene            complement(31106..31182)
                     /locus_tag="PLW13_01480"
     tRNA            complement(31106..31182)
                     /locus_tag="PLW13_01480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31146..31148),aa:Pro,seq:cgg)
     gene            complement(31243..31425)
                     /locus_tag="PLW13_01485"
     CDS             complement(31243..31425)
                     /locus_tag="PLW13_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3094 family protein"
                     /protein_id="HQY69070.1"
                     /translation="MSEAGKLHPEDQQRVDAFTSTGIHSVPRKPFRPLVLLAVILVAT
                     TAMTVLSIIIERMYIP"
     gene            complement(31528..33324)
                     /locus_tag="PLW13_01490"
     CDS             complement(31528..33324)
                     /locus_tag="PLW13_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQY69071.1"
                     /translation="MLSYKAPLRDMSFVRDELLGFPALFAALPGCEEFTPDLIDAILG
                     EAARFCENVLAPLYRSGDEEGCTWTPEGVRTPAGFREAYAQYVEGGWAALSTPPEYGG
                     QGLPPSMGLFVEDMMGTANWAWTMYPGLSHGATRTLKVHGTDEQKQTYLRHLVSGEWT
                     GTMCLTEAHAGSDLGLLRTRAEPQADGSYRITGTKIFISAGDHDMAANIVHIVLARLP
                     DAPPGTKGISLFLVPKKLPQADGTLVPNSVSCGSIEHKMGINASATCVMNFDAATGFL
                     IGAPNRGLNHMFTFMNAARLGTAMQGLAAAELSFQGALAYARERLQGRSLSGPKNPSG
                     PADPLIVHPNIRRMLLTQKAYAEGSRAFLYWLCQLVDIADKSADAAERKRAEDLMALL
                     TPVAKAFVTEAGYEAANLGVQVFGGHGYVREHGMEQIVRDTRIALIYEGTSAIQALDL
                     LGRKIMGSGGELLHGFAAIVEQFCSENAGNADMAPLVAALTATGKRLEGLTMHIAGAA
                     MKNPDEADAAAFEYLMFMGYFVLGFMWARMARTALDALAGGTGEADFYTAKIETARFY
                     FQRILPRTESLALCVEAGGTTLMALPEAHFAL"
     gene            complement(33414..33644)
                     /locus_tag="PLW13_01495"
     CDS             complement(33414..33644)
                     /locus_tag="PLW13_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69072.1"
                     /translation="MSSTPIGQTGFQGLRDSLERAQAAATRVVSSAAGGDAGQLTEAI
                     VELQAAKLQAGAAAQVVRAADDTLGTLLDVLA"
     gene            complement(33717..34412)
                     /gene="bioD"
                     /locus_tag="PLW13_01500"
     CDS             complement(33717..34412)
                     /gene="bioD"
                     /locus_tag="PLW13_01500"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQY69073.1"
                     /translation="MAEAWFVTGTDTGVGKTLVAAALLRAAGASGARTIGLKPIASGA
                     VATAEGLRNEDACALLAAMNQELPYALVNPVVLAPAVAPHIALAEAGLALDVAALHAR
                     CLPALARPHDVAVVEGAGGWRVPLNHAETLADLARRLGFPVVLVVGLRLGCINHALLT
                     AEAVRADGLRLAGWVANQVDPGMANLADNLATLDAMLGAPRIGFIPWRAGIDVRTAAG
                     LLDIGRLRRGAQD"
     gene            complement(34405..35223)
                     /gene="bioC"
                     /locus_tag="PLW13_01505"
     CDS             complement(34405..35223)
                     /gene="bioC"
                     /locus_tag="PLW13_01505"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HQY69074.1"
                     /translation="MTCFATPLAAAARSKQDVARSFGRAAAGYERAAALQRTIGEELL
                     ALAPPLDPARVLDIGSGTGHFSARLAQRFPRAGVIGLDLAEGMLRQARAGAPGGATSW
                     LCADAEQLPLAGASIDLVYSSLALQWCESPVRALAEIARVLAPGGQALIATLGDDTLW
                     ELRAAWRAVDDGVHVNRFESVAALERAARAAGLAVRVIGQKYHLPAYPDVQGLARELR
                     AIGAHNVNAGRPGGLAGRGAWRRLQSAYALFANPDGTLPATYRALCLRLEKPRG"
     gene            complement(35254..35994)
                     /locus_tag="PLW13_01510"
                     /pseudo
     CDS             complement(35254..35994)
                     /locus_tag="PLW13_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(35991..37157)
                     /gene="bioF"
                     /locus_tag="PLW13_01515"
     CDS             complement(35991..37157)
                     /gene="bioF"
                     /locus_tag="PLW13_01515"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQY69075.1"
                     /translation="MAGAFASLGADLAARAAAGLYRGRRVLEGPQATTVRVDGRELLA
                     FCSNDYLGLANDPRVIGALQQGAARYGAGSGASHLVSGHSRAHHELEEALAQFTNRPR
                     ALLFSGGYAANLGVISALLGRGDAVFQDRLNHASLLDGGLLSGARLQRFLHNDAASLE
                     ARLARSPARRKLVAVDGVFSMDGDEAPLAALAAVCARHDALLYVDDAHGFGVLGERGA
                     GTLEKQGMGSAGVPLLMATLGKALGVAGAFVAGSEELIETLIQFARTYVYTTAMPPAL
                     AVAAAESLRIVREEPWRRAHLQELIGRWRAGANALGLQLLPSQTAIQPLLLGDAATAL
                     RWSEQLLARGLLVPAIRPPTVPRGQSRLRVTLSAAHTVAEVERLLQALADIAGA"
     gene            complement(37244..38209)
                     /gene="bioB"
                     /locus_tag="PLW13_01520"
                     /pseudo
     CDS             complement(37244..38209)
                     /gene="bioB"
                     /locus_tag="PLW13_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
     gene            38289..38984
                     /locus_tag="PLW13_01525"
     CDS             38289..38984
                     /locus_tag="PLW13_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQY69076.1"
                     /translation="MVYKSLLRGLLPATCLLCGGAARAALDLCADCAAQLPLLDNACP
                     RCAMPLADAHGAPCGPCLQRAPRFGACIAALRYEHPVRELVSRFKFQGDLACGRMLAG
                     LLARRLAALPPARRTGLVLVPVPLHPARLRERGFNQSERIARVLAAELGLALSNTLVR
                     RVQRTADQKQLGAAARSRNLQGAFHATGCAGRRIALVDDVITTGATAEAVTAALFAAG
                     AAQVEVWCLARAL"
BASE COUNT         5797 a        14374 c        13722 g         5240 t
ORIGIN      
        1 gcgccaactc gtctttcagt tcgtcgagat gcgctttccc catcagggta tcggcctctt
       61 tggcgaacga tggcgtctca acgatggttc gcactcaggc tgcctgcgcg ctgtagtcca
      121 ttggagtata gccgcctcgg cgagttccgg tcacgcgccg aaacgaggcc cccagcgcgc
      181 gagaaacggt cccgaaacgg tcctgttttt tggcggtctg tgcgcttgtt agcgcccgct
      241 taccgtcagg gcctagggag aatggagctg gcgagaggaa ttgaaccccc aaccggctga
      301 ttacaaatca gctgctctac caattgagct acgccagcgg ctcgattctc tcgtcccgcc
      361 ggacggcggg gcgcgaagtt tagtcggagc ctgtcgcggg tgcaatgccg tctgtaaggc
      421 cgtctgcaag ggcgtcacag cccaccggcg cgacgccggc cccggacgtg gtcgccgcga
      481 cggcctcgac gagctccggc ggcacggctg cccccgcatc gggcccaagc gcgccgagca
      541 ggaagaatgc ccggcgagtg cgcggcttct cgtgaatcgc caccgggtaa ccgatcccct
      601 gcatgcgttc catctgttgc tgcgcgctct gcgcatcgcg gaaaacgccc agcgacacac
      661 catgcagcag caccggttca tcgcggatca cgaagctctc gacgccgcgc gccgtcagcg
      721 cgctcaccac ggccctcgct tcgtcgtcgg acggacgcgg gggcacgtgc acccagaagg
      781 tactgcgatc gggcgcctcc ccctcctcga cccgggccgc gggcaaccag gcagctgcgc
      841 gctggcgcat gcgctcgccc gtggcgccgt cgcgcagcgg cccgatccgc acgcaatgtt
      901 gctccggcgc cgcgaccgcg tcggtctcct gcgccgctcc ggccgcggca gggtcatccg
      961 cccgcgcgtc gcgatgctcc ccggcgggcg gggaggcatc gggcaattcc cgcgcgtccg
     1021 gctggccagc cacgccgggc gctgccgatg gatccgctgc agcggatcca gcaggaaccg
     1081 atccgggcgt tgcgatggcg tcgcccgcgg gcgccaatgc cgcggtggcg tcgccggcct
     1141 ccccgggcgc cgcgatggcc atgctctccg ccgcaacctc tggcgcaggt gagtattcac
     1201 tcgcaagttc cagcctcaca gcataagcag gcgctcccgg gcggcgcccg gcgtcctcga
     1261 tgctctcgcg cggcgacaac aggagccagc ccgcgcagag cgcgttggcg gccaggagca
     1321 ccaggaacag gcggcgcatc agggcagggc caggctgagc ccgtcgagca ccagctgcgg
     1381 ccggatgttc gcggggaagt cgaggcaggc agcgatggcc ggcgcatcgc cgccggtcag
     1441 cagtaccacc gctccggggc acgcctgcgc gaagcgctcg cgggcagcgc gcaggaagcc
     1501 ggccgcggcc agcagggtgc cgccgccgac ggcctcggcc gtgtcgcgcc ccggcgccac
     1561 ccccgttgcg gccgccgtgg cttcgaagcg cacgcggtcc gtggccgcaa gcaggctggc
     1621 gcgcatcatc gtgagaccgg gcgcaatgta gccaccgcgg tgctcgccgc cggcatcgac
     1681 caggtcggcc gtgatcgcgc tgccgcagtc gagcacgcag accgcgccgc cgatctcggc
     1741 gaaaccggcc agcatcgcga gccagcgatc gaccccgagc cgttgcggtt ccgcgtagcc
     1801 attgcgcacc ccggcggcga cggcctgcga gcgggcgaac gccgtttcca gccccagcac
     1861 ctcctgcacc gcctcgcgcg tcgccgcctc cacctgcggc ccgcgcacgc agccggcgcg
     1921 cacgcgcgcg atcgccgcgc gctccggcag cgaacccagg gtggcggcaa gttcatcgac
     1981 ggcgcacgcg ccggcacgcg cgggcgcctc tgccaggcgc cacttgcagc gcgtattgcc
     2041 gatgtcgagc tccaggatca cccggtgcgc ctcacgctta tctcgccacc gctcagcagc
     2101 tgctcccctt cggaggtcat gagccgcaac gcgccggtgg catcgatgcc attcgccata
     2161 ccctgcacgc tcgggccgct tccgcccgac accgacactt cgagcccgcg cagcgcatcg
     2221 acccgctccc actccgtggc gaaatgaccg aaaccgtggc gcgcgaaatc gcccaggata
     2281 tcgaggcagg actccagcag cgccgccgcc agcgcgttgc gcccgacgcg ggcgccgcac
     2341 accgtctcga gatcggtcca ggactgcccg attgccgtgg cggcgccggg cggcatgctc
     2401 acgttgatgc cgacgccgat caccgcggtg acagggcctg ccagctcgcc gtcgatctcg
     2461 atcagtatgc cgcccagctt cgccgcgccc gccaccaggt cgttcggcca cttgagcatc
     2521 acgtcgcgcg cgcccaagcg cgtgagcgcg cgcaccgcgc ccatgccgat caccaggctc
     2581 aggccctcca gcgccgccac gccgccatcg aagcgccagg cgaaagagca gtagatgttg
     2641 cgcccgaagg gactcaccca gctgcgtccg cggcggccgc ggccgctggt ctgctgttcg
     2701 gcgaatacca cggccccatg gccgagcgtg ccggcccgca agccttccag caccagcgca
     2761 ttggtggaat ccaccacccg gcgcaactcg agccgcgaca gctgttgccg cacccgcccg
     2821 ggcagtgccg ccgcgatgca ctcgcgatcg agcagctcga tgccgccggg cacgcggtag
     2881 ccgcgcccgc tcacgcgctc gagcgcgatg ccgcgctcgg cgagcttgcc cacgcgcttc
     2941 cagatcccgg cgcgggaaat gcccagccgg cctgccagcc actcgccgga gcggaactcg
     3001 ccgtcggcga gcagcgccag caacgcggtt tcctcgttga ggtctgccac gggcaacggc
     3061 cggtgcggcg ccggatcggg cggatgccgg ccactagtca cggtagcgcc gcaccaggtc
     3121 ctggtaggcg tcgatgcgcc ggtcgcgcag gaacggccag atgcgccgca cgtcctcgct
     3181 gcgcctggag tcgagctccg ccagcaatac ctgcggctcg ctgtcgccgg cgcgcgcgat
     3241 gaactcgccc tgcggaccgg caacgaagct gtgcccccag aagcggatgc ccggcccctg
     3301 cgccgagggg tccggctcgt ggccgacgcg gttggcgacc agtaccggca cgccgttggc
     3361 gaccgcgtgc gcgcgctgca ccgtgatcca ggcctcgcgc tggcgagcct gctccccggg
     3421 cgtgtcgcgc gggtcccagc cgatcgcggt cgggtagagc agcagctcgg cgccggccag
     3481 cgccatcagg cgcgccgctt ccgggtacca ctggtcccag cacaccagca cgccgaggcg
     3541 gcccaccgag gtgtcgatcg gcgtgaagcc gagatcgccg ggcgtgaagt agaacttctc
     3601 gtagtacgcc ggatcgtcgg ggatgtgcat cttgcggtag cgcccggcga tactgccgtc
     3661 gcgctcgaac accaccgcgg tgttgtggta caggcccggc gcgcggcgct cgaacagcga
     3721 ggcaacgagc acgatgccga gctcgcgcgc cagcgacccg aggaattcgc agctcgggcc
     3781 cgggatcgct tcggcgcggt cgaaacacgc cacgtcctcg acctggcaga aatacggccc
     3841 ggtgtgcagt tcctgcagca gcaccaggcg cgctccgccg gccgcggcct cgcgcaccag
     3901 cgcggccgtg gtcgcgagat tggcgtcgcg ccagtccacg caggcgtgct ggatgcaggc
     3961 aacgagcagc ctggaactca tgtgcttctc ctcacgatgc gccctcatga tgcgaccccg
     4021 tggtgccgcc tcgcgtggcg gcctcgaatg gcgcgtgaaa attataccgg cgccagcgtg
     4081 ccggccggca actgcatcgt gatgcagtgc agcgagccgt actgctggat caacggcagg
     4141 cagtcgatcg cgatgatctc gcgatcggga aagcagaacc gcaactgcgc cagcgccccg
     4201 gcatcggccg ggtcgcgata ggtcggcgcc agcaccgcgc cgttgatgac caggaaattg
     4261 gcgtaggtcg ccggcaggcg ccgcccgtcg cgtccgtgct tggcgcccgg ccacggcagc
     4321 ggcaccaggc ggtagggctc gccgcgcgcg tcgcgcagcg cgcgcagttc gcgctccatc
     4381 gcgtcgagct ccgcgaagtg cgggtccgcg cgctcgctgc agcgcacgta ggcaatggtg
     4441 cgttcgtcgc aaaagcgcgc cagcgtgtcg atgtggctgt cggtgtcgtc gccctcgagc
     4501 gcgccgtgct ccagccacag cacccgttgc aatccgaaca gctctcccag gcgctgctcg
     4561 atcgccgcgc gcgaaaacgc cgggttacgc cccggcgaca gcaggcaggc cgaggtggtc
     4621 agcagggtgc cggcgccatc ggactcgatg ccgccgccct ccagcacgaa ctcgaccggc
     4681 tcgcgcggcg tgtcaccgaa ggcgccctgg cgcgccagct ccgccgtgat gcggtcgtcg
     4741 agcccggcct cgtacttgcc gccccagccg ttgaacacga agtccagcag gcgcggccgc
     4801 ccgccgtcga tgatcgtgat cggcccgtgg tcacgcgccc aggaatcgtt ggatggcacc
     4861 acgcgcagcg ccacgcgccc gcgcggcacg ccgcctgccg ccagcagggc cgccacgtga
     4921 tcgcggtgcg ccgcgtcgcg cacgcagacc agcacgcgct cgcgcgcgct caccgcatgc
     4981 gctatcgcca cgtagaccgg ctcgacctgc gccagccacg gcagccagtc gctgtgctcg
     5041 tgcggccagg tgagcatgat gccgtcctgc ggctcccatt ccgccggaag ccgccgcgcg
     5101 gggggcgctg ctgtctgcat cggtcgttcc tcgaagaatc tgcgacatcg ggcggcgctc
     5161 ggacccgccc cggacgggcg cgcagtctaa tccatcgccg cggacggtgt cgccggctca
     5221 attcggcgcg cgtcactggc ataatcgggc gccgcgccac cgcggcaacc caggacagcc
     5281 gagaccttgc ccatcacccg gaatcagcgc aaccaccgcc gccgcggcgc cctggcgctg
     5341 ctgtgcgccg cggcgttgct gtgcgccctg gcgctgcgcc cggcggcagg tctcgcggaa
     5401 ccgctgaagc tcacggtgcg cgggctggac aacgcggcgc agcagaacct gcgcgcgcat
     5461 ctcggcgatg tcgatgtcgc actcgccggg caggaggcgc gactgcagag agtcctgacg
     5521 cgcgcggtgc gcgacgcgct gcagccgctg ggctactacg aggcgcagtt cagcgttgcg
     5581 cgcgacggcg cgcggctggt gatcgacgtc gagcccggcc cgcgggtgcg gtttgccgcg
     5641 cccgagatag ccgtcgacga gcccgccgcc tcgttgccag ccatccgcgc gctgctgcgc
     5701 tcgggcgcgc tggccgcggg caggccgctc gcccacgccg actacgaccg ctttcgcgac
     5761 gagctgctgc gcggctgcct gcgtctgggt ttcttcgacg cgaagtaccg gctctcggag
     5821 ctgcgcatcg acctggcggc gcgcaccgcc accgccaggc tggacctcgc ctgcggcgcg
     5881 cgctacgcct tcggcacgct ggagatttcg ggcagccgga tcgatcacga gctgctcgca
     5941 tcgctggcgc ggttccgtgc cggcgagccc ttcgacaacg agctcgtgtc gcgcttcgag
     6001 cagcgcctgc gcgacaccgg cttctttcgc gaggtgatcg tgcgcgtgac gcccggggcg
     6061 gatgcccgcg cggacgtgac cgcgctcacc gaggacatcg tgaccagccg ttacgaggtg
     6121 ggcctgggct tcagcaccga ctccgcgctg cgcctgcgct tcaaccgcga ctcgccgctg
     6181 ctcaacgaca acggccattc gctgcgcatc gagtccgaga tcaccgagtc gcgccaggcg
     6241 gtcgaggccc tctaccgcat tccgcaccgc gatccgctcg acgacatcct cgagctcacc
     6301 gcggggatgc aggaccagag cgtgcaggac accgactccc tcaccgcgac gttcggcgtg
     6361 cgccacgcga tcaaggtctt cggcgactgg tccttcggct acggcgccag cgtggagatg
     6421 gaacgctaca ccatcggcag ggcgagcgag aagcgcaccg cctacctgat gcccgcggcc
     6481 agcatctcgc gcacgcggat cgatcccggc gtggatccgc gcagcggcag cgcgtggttc
     6541 agcgcgatcg acacgagcga ccgcgcgctg ggctcgccgg cggatttcct gcgctggcgc
     6601 ggcagcggca agtggctcgc cggcctgccc gatgacaaca ccacggtgct cgcgcgcctc
     6661 gagctgggcg cgatcctcac cgacggtttc gagaacctgc cgccgttgct gcgcttcagc
     6721 gccgggggcg acaacagcat ccgcggctac gatttcgagt cgatcgggcc acgcgacgac
     6781 aacggcaaac tcaccggcag ccgctatctc gccgtaggca gcatcgaggt atcgcggcgc
     6841 gtgctgccgc aatggcgcgt cgccgccttc gtggacgggg gcagcgcgtt caacgactcg
     6901 aacgccgcgt tctaccagag cgccgggctg gggctgcgct ggctgtcgcc ggtgggccag
     6961 atccgggtcg atctcgcggt accggtgcgc gacggcgaga actcgggttt caaggtgcac
     7021 atcagcatgg ggccgccgct gtgagcgcgc gcggcctctc gctggcgctg ctgcgcgtgc
     7081 tgctgtgggg cctcaccgcg gcgctggtgc tggcgctggg gctgggcgcc accgcgcgcg
     7141 gcacggcgct gctggcgcgc acgctgtctc tcgtggagcc ccggctggtg atcgcgcacc
     7201 gcgacggcaa cctgctcgag gcaggcttcg agcgcatcgc ctggcacgac gccgcactcg
     7261 cgctcgagct gcgcgatgtc gcgtggacgc ttacgccgcg ctgcctgctc accgacgtgc
     7321 tgtgcttcga acagctcgcg atcgccacgc tggacatcga catcggcccc gcctcgggcc
     7381 gatcgagccc gctcgcgctg gcgccggtga tgctgcccct gcagctcagc gtcgcgcacg
     7441 gcacgctcgc caccctcaac gtgcgccgcg gcgccgcgcc gctgctgacg ctgcaagaca
     7501 tcgcgctcgg cggccagatg cacggcagtc gcatcgagct gcaacgcctc gaggcgcgca
     7561 tgggcgagtt gcgagcctcg ctggcgggca gcgtcgagct gcacgcgcaa ctgccgctcg
     7621 aagccacggg gcgcctgcgc tggggcgccg cgcatcacgc cgacatccgc gccggcgggg
     7681 acctggcgcg cctcgtgttc agcgcgcgca gcggcggccc ttacgcgctc gatgtcgagg
     7741 gccgcatcga tgcgctggcg cgcccgctcg cgctcacgct gacggcgcgc agccgcgaga
     7801 cgctgctgct gcgcccggaa cagcccggtc ttgccgcgct gcacgaggcc acgctgacgc
     7861 tagagggcac gctggccgcg ctgcgcgcca cgctcgcggc gcggctggag tccgaggcgc
     7921 tgggagccaa cgcagtcacc gccgccgcga cgtggacgcc cgggcgcatc gacgtcgagg
     7981 ccttcgcact gcaaggcgag gcggggcagc tggacgccag cggccatctc gacaccggcg
     8041 ccgggcgcca ttgggtggtg caggcgcggg ccaaggagtt ctgtcccacc ggctggaccc
     8101 cggcgctgcg ctgcgccctg agcggcaccg ccacgctgga gggcgcattc ggcacaccgc
     8161 tcgcgctcgc cgtcgagagc gcactcgagg gcaccgtcaa cgaccacccc gcgcgcattg
     8221 ccgggcgcgt ggcggcgttg ccggacgggc gctggcaagt cgacgggctg cggctcgcga
     8281 gcggcgcgaa cacgctcgcg ctcgagggca gcgtcggcga gcagatcgcg ctcgaggcca
     8341 cgctcgaggt cgccaatctc gccgacacca tcgccactgc caccggctcg ggcggcggca
     8401 gcttgcgcgt gcgcggccgc cgcacagacc cgcaactcca gggcacgcta cggcttcggg
     8461 acgcgacatg gaacggtatc gaggccggtt cgctggtgct ggaggcggac tggcgcggac
     8521 tcgcgcaggc gcgcaacagc gtgcggctcg cgctcgacgg tgcacgctgg cgcgacttcg
     8581 cgggcgtcgg cctcgaagcc tcgctcacgg gctcgggagc ggcgcaccgc atcgcgctcg
     8641 aggcgcgcgc ggcggacgcg gcgctgcggc tcgattgcgc gggcgccctc gccgtggcgc
     8701 agggctggcg cggcgaatgc cggcgactgg atgtcgcgcc ggccgtcgcg caagcaccgt
     8761 ggcgcggcga tcgcccgctg cggctggcgt ggcagcgcaa ggacggcgaa gtgcgcatcg
     8821 agccgttctg cctccggcgt gacgccgcag cgctgtgcag ccgccgcacc gcggtcatcg
     8881 gcgcgggacg cctggccggc atcgcgatcg atggcagcga catcccgttt gcgtggctcc
     8941 gcccctggct tcccgcggga ctggagctcg gcgggaactt cgacctgcgc gtggaagccg
     9001 acaagggcgt cgagcgcaac ccgcgcatcg aagcgcgcgc cagcaccggc gcgacgacgc
     9061 tcacggtgct cgcggcgggc gaagcgctcg acatcccgct cggcggcgcg agcgcgtcgc
     9121 tgcgcaccgc cggcgccggc atcgcgctcg actggcaact gttgctcggc ggcgacgcga
     9181 gattcaccgg tgaggccctg ttgcagcagg acagcggcgc gctgtcgggc acgatggcgc
     9241 tgcgcgggct cgacctcgcg ccgttcgcga agcaggcgcc gggcgtggtc gcggcggcgg
     9301 gcacgctcgg cggcgacgtt cgcctcgggg gcagcctgcg cgaaccgcgc gcctccggcg
     9361 tgctgagcct gcgcgacgga atgctgagcc acgagaagct gccgcacccg atcgaggaca
     9421 tcgtgctcga cgtggagttc agcggtgccg aggcgcggct cgcgggccgc ttcggctcgg
     9481 tcgccggcgc cggcaccctc gagggcaccg cgcgcttcgc cggcgccggc gccggctgga
     9541 gcgccgatct cggcctggaa tccgccggcc tgcggctcga gccgatgcgc ggcagcacgc
     9601 tcacggtggt gccggacctg cgcgtgcagc tcggcccgcg gcacgccggc atcagcgggc
     9661 aactgttcat accgcaagcc gacatccgcc tcgaccggct cccggccagc gccatcagcg
     9721 tatcgcccga cacagtcatc gtgggcgagg aaccggcggg cgcgccgttc gactacagcg
     9781 cagacctgga ggtgcgcctc ggcgacaatg tgcggttgcg cggccggggc gtggacgcac
     9841 ggctcgaggg ccgcatcgag gtgctccgcg acatcggcga ccgagagctg cgcggacaag
     9901 gtgagatcga aatcgcggac ggtcgcttca ccgcctacgg ccagcgcctc gaggtgaccg
     9961 agggcgtggt gcgctttcgc ggcccgctcg accgtccgca actgcgcctg accgcggtgc
    10021 gccgcatcga cgacgagaac atacaggtcg gcgtgcgcgt acgcggcgac ctgcgcgatc
    10081 ccaagctcag caccttctct cgtcccgcga tggaggaaag ccgcgccatg cactacctgc
    10141 tcaccggacg cccgccctcg gccggcggca actccgacct ggtcgtggcg agcatgctga
    10201 tgcaactcgg cgtggccggc gcgagcggcg tcaccagcaa tgtgctgcgg ggtttcggca
    10261 tccaggattt ccagctcgcc acgcgcgagg tcgagggcgg caccgaggtg cacctgagcg
    10321 ggtacctgcg gcccgacctc tacctgcgct acggcgtgtc gaccttcgac aaggtcaaca
    10381 ccttcaggct gcgctaccgc ctgcgccgtt cggtctatgt cgaggccgtt tccggcatcg
    10441 agaacgcggt ggacttcctg tattctttcg agcgctgaga ggcgcgccac gctgcgcgcg
    10501 ggcgacaagg agcacgcagg accatgagca tcagcgcccg tttcgaggtg gccttttcgc
    10561 gcttcctcgc gcccgagggc aacgtcgaac aggaactgcc ccccttcgcg cgcgagcgcg
    10621 atgccgtgct gcggctgtat cgcgccatgg tgctgacgcg aagcttcgac acgcgcgcgg
    10681 tcgcgctgca gcgtaccggg cgcctcggca cctacgcctc ctcgctcggg caggaggcgg
    10741 tggccgtcgg tgcgggcgac gccatgcagg ccggggacgt gctggtgccc tcgtttcgcg
    10801 agcacggcgc ccagtggctg cgcggcgtca cgctgcccga gatactgcaa ttctggggcg
    10861 gcgacgagcg cggctcctgc ttcacggcgc agccacgcga tttcccggtc tcgatcacgg
    10921 tgggcgcgca cgcgacgcac gccgtcggcg tggcgcacgc gatgcgctac cgccgcgagg
    10981 cgaacgccgt ggtctgcgtg ttcggcgacg gcgccacctc aaagggcgat gtctacgagg
    11041 caatgaacat cgccggcgcg tggcgctcgc cggtggtatt cgtggtcaac aacaaccaat
    11101 gggccatctc gatgccgcgc gccgcgcaga gcgcagccgc aacgctggcg cagaaggcca
    11161 ttgccgccgg catcggcggc gagcaggtcg atggcaacga cgtgatcgcg gtgcgccacg
    11221 ccgtcgcggc ggcgctgcaa cgcgcccgcg ccggcggcgg cgcgcacctc atcgagtgcc
    11281 tgtcgtaccg cctctgcgat cacaccacgg ccgacgacgc cagccgctac cgcgacgacg
    11341 ccgaggtgag cgcgcactgg aaggaggacc cgctcgcgcg cctgcgcacc cacctgggcg
    11401 cggcgtggaa ctggtcgcgc gaggaggagg aagccttgca gcacgagtgc gcccgcgcgg
    11461 tcgaggaggc cgccgcggcc tggctggcca cggcgccgca gccgccacgc gcgatgttcg
    11521 atcatctcca cgcgcgcctg ccgctggccc tggagccgca gctcgccacg ctgcagcgcc
    11581 acgacggcgg caccaaagcg catggctgag ctcacgctgg tcgaggcggt gaaccgcgcg
    11641 ctggcgcgcg cgatgcagga cgacgcggcg gtgatcgtgc tcggcgagga cgttggccgc
    11701 gacggcggcg tgttccgcgc caccgagggc ctggtcgaac gcttcggcgc cgagcgcgtg
    11761 ctggatacgc ctgtcgccga ggccgcgatc gcggggatcg ccatcggcat ggcggcccac
    11821 ggcctccggc cggtcgccga aatccagttc gacggtttca tgtacagcac gctcgaccag
    11881 atggtgaacc acgccagccg cctgcgtaac cgcacgcgcg ggcgcctctg ctgcccgatg
    11941 gtgctgcgcg cgcccttcgg cggcggcatc cacgccccgg agcaccactc cgagagcccc
    12001 gaggcgatgt tcgcgcacat tcccgggctg cgcgtcgtga ttccgtcctc gcccgcgcgc
    12061 gcctacggcc tgctgctcgc cgccatccgc gatcccgacc cggtggtgtt cctcgaaccc
    12121 aagcgcagct accgcgcgct gcgcgaggag gtcgccgacg acggcgtcgc gctggcgctc
    12181 gacacctgct tcacgttgcg cgaaggcagc gacctcacgc tgatcagctg gggcgcgatg
    12241 ctcggggaaa ccctggccac cgccgacgcg ctggcggcgc agggcctggc cgccgaggtc
    12301 atcgacgtcg ccacgctcaa gccgctggac gcggacacca tagcggcctc ggtggcgcgc
    12361 accggtcgcg cggtgatcgt gcacgaggcg gcgctcagcg gcggtttcgg cgccgagatc
    12421 gccgcgcggc tggccgagcg ctgcctgttc gaactgcgcg cgccggtgct gcgcgtcgcc
    12481 ggctacgaca ccgtcatgcc gctggccaaa ctggaacagc actacatgcc ctcacgggag
    12541 cgcatcctcg aggccgcgca cgaggtcatg cgatacccat gagcgacacc gaattccggc
    12601 tccccgatct cggcgagggc ctgcacgacg ccgagatcgt gcagtggcac gtggcgcccg
    12661 gcgatcacgt ggtcgccgac cagccgctgg tcgcggtgga gaccgacaag gccgtggtcg
    12721 agatcccggc gccgcaccgc ggacacatcg ccacgctggg cgcggcgcaa ggcgagcgta
    12781 tcgcggtcgg cgcggtgctg gtcacattca tcgcgacggc gcatgccgac accggcgcgg
    12841 tggtcggcga gctgccgctc gcgcgggccc cggtggccgc gacaccggcg cgccccgccg
    12901 ggtccatcca gcacctgcgc gcctcgcccg ccgcgcggcg cctggcgcac gaactgcgct
    12961 gcgagctcga gagcatcacc ggcagcggac ccgatggcgt gatcagcgtg gccgatgtgc
    13021 agcgcgcggc ccaggtcgcg agcgctcccg ccgctgccgg ctgggaaagc ctgcacggcg
    13081 cgcggcgcgc gatggccgag cgcatgagcg cggcgcatcg cgccgtggta ccggcctcgg
    13141 tgacgctgca tgcccgcatc cgtgcctggc acggccgcga tcgcccgctg ctgcgcctga
    13201 tccgcgcgct gctgcacgcc tgcgccgtgg agcccgcgct gaactgctgg ttcgatgccg
    13261 ggcgcgaggc cgtgctgcgc cacgacaccg tgaacctcgg cctcgcggta aacaccgccg
    13321 agggcctgtt cgtgccggtg atcgccgcgg ccgagcgcct ggacgacgcc gcgctgctcg
    13381 cgacggtgca ggcgctgcgc gaagccgtgg cctcgcgcag catcgcgccg gcgcagctgc
    13441 gcgaggccac gatctcgctg tcgaacttcg gcacgatcgg cggcgagacg gcgcagatgg
    13501 tggtcgcccc gccgcaggtc gcgatactgg gcgccgggcg catcgcgccg ggggtcgtcg
    13561 cgcgcgacgg cctgcccgcg gtggagccgg tgctgccgct ctcgctcacc ttcgaccacc
    13621 gcgccgtcac cggcggcgag gcggcgcgct tcctcgaggc cgtgcgcgcc cacctggaac
    13681 aggagacctg cgcatgacgc ccgacagccc cgtcatcatc gagctcgcga tcaacggcgt
    13741 gacccagccc gcgcgcaatc cgcacgtgcc gctcaccgtg gaggccatcg ccgcggatgc
    13801 actcgactgc gtgcgcgccg gcgcggcgat cgtgcacagc cacgcgccgc tcagcgattt
    13861 caacctgccc gcggagcaag ccgccgaggc ttacctggcg agctaccgcg cggtgctggc
    13921 gcagcggccc gacgcgctgc tctacccgac catcggcggc ggcgccacga tgaccgaacg
    13981 cctgcgccac atcgagatcc tcgccgcggc cggcgcaatc cgcatcgggc tgctcgatcc
    14041 cggctgcacg ttgctcggct gggccgaggc cgacggttcg ccgagcccgc acagctttgc
    14101 gtacgtgaac accgtcaccg acatggaggc agcgctggag cagggtgcgc gcctcgggct
    14161 ggggctcagc atcgcgatct acgagccggg tttcctgcgc aacgcgctcg cctactggcg
    14221 cctcgggcgc atcccgcgcg gcagcatgat caagttctac ttcggcggcg aggcgggtta
    14281 cttcggcggc ggcagcggcg tgagcttcgg cctgccaccg accgcgaaag cgctggatgc
    14341 ctacctggag atgatggaac tggccgcctg cccgctgcca tggtcggtgg cggtgatggg
    14401 cggagacctg ttcgcgacgc cgctggcgcg cctcgcgctg gagcgcggcg gtcacctgca
    14461 caccgggctg gaggaccacc tgggcgccgg gcagcccaac aacctggagc tcacgcaacg
    14521 cgccgtggcg ctgtgccgcg cggtgggacg cccggtggca gaccctgccg aggcggcgcg
    14581 catcctcggc ctgccctgac tcgcgcgcgg cggatcagct gacgtcaggc agatcgcagc
    14641 gcctcgtcct ggcgcggaaa tcccggttca tgcgccgcac cggctccagc agcggaccgg
    14701 gctgtccctc gagatcgtag atcccgccat attgaacgtg gtcgaagatc atgagctcgc
    14761 gcagcgcgag ttcgccgtgg cgcagctcca gcgcgaacgt aaggccggcc gcgtcgggct
    14821 gcgccgcgat agcggcgcaa cggcgctgca ggccggagcc gtcgaattcg agcaccaccg
    14881 gctcgctgcc ggcacgcacg gccaaggccc cgggcgcgcg cacctcggcc tcgacgcaga
    14941 cgcggtcggg caggtcgtcg ccggcgcccg ggaggcgccc gcgctggcgc agcgtcacct
    15001 gctgcgacag acggtcaccg gcgagcaggc gcagctggaa gtcaccgctc gccagctctt
    15061 cgagcgcctg ttccggcacc ccggtcgccg tttcgagccg ccaaccttcc agcccgtaac
    15121 cgaaggccgg gttgtgcagg ggactctcgg cataatcgcg caccgtccac agcccgtaac
    15181 ccagcacctc gcggcgcttc aggacgcaca ccgcggcgtg caggaaggcg gcaacttcct
    15241 cggggcggat cacggcgttg ttgccatggc ccacggtgtt gtccacgaag ttgaactggt
    15301 cgatgaacag ccggcgtccc gacgtggcgg ccgactccac cagcagtgcg tccagcaagg
    15361 cgaggctgcg cgtggccggc agttgctcac cccggttgtc cgatccccag aacggggccc
    15421 agtagatcgt cgacgggtac cctggcgcaa tccgcatcat gccgtcgtga ccgatccacc
    15481 cggccaccac gggctggccg tcgggaccgg gttcgaaacg cggctcgtca tcgacgcgcg
    15541 cctcgaacga gagcagcggg aacagcgcca tcgcgggctc gagcaacttc gcgatcacga
    15601 gccagtccca gtaggcattg aacagcacgg ggtcgcgcgc ggcgtccggc agtgccggcg
    15661 gatgcgccgg gccgggcgac agcgtggaca ggtattccga ccaggtgccg gcggcggcct
    15721 ggtccacgcg gcgcagccac aggtcctccc agctcatgaa cgccatcacc accccctcgc
    15781 ggccctcgac ctcctgcgac acgcgcccca ggaaaccgaa gaacgcctcg cgcgcctgcg
    15841 cgtcgttgag caggcgctgc tggcgctcca ggacgctgcc ggcctgcgga tggaagctcc
    15901 agccatagcc gaggcgcagc atcaccttca acccggcctc gtcggcgcga tcgagcagga
    15961 agcgcaggcg ctcgaacgcg cgttcgtcgt aggtgcagca cggcgcgtac acgggctgga
    16021 agtcgcccca tgacaccagc agcaccaccg tgtcgaagcc gtcgtcgacg agttgccgga
    16081 aatcggcggc cacctgctcg cgccggaatc ccgccaggaa gttcatcggc cactgccggc
    16141 cgaaccagtg ggcggcaacg cgaccgcgca cggcatcgtc gggcggtatc gcggggctgt
    16201 tctgcaccgc tgcgggcgat gcgggcgatg cggacgccgg gacgctcgcc gcgagcacgg
    16261 caaggctggc agcgacggca aggacgctcg ggcgaaggtt catgcacggg gatccggaag
    16321 gcgggaaccg gattataggg cgagggatcc ggcaaagcct gtgcgcgcgt gcccgctatt
    16381 gcggctgccc ggccaggccc atcatttgcg ccgacagcag gatcacctcg atctcctcgg
    16441 tgatctcctc ctggcgccgc cggttgcagt cgagccgcag cttcccggtt tcctcctcca
    16501 ggcgctgcag cgcgcggtcc atgtgcgcct ggcgctggcg gttctcggcc atgagcgagc
    16561 tgtagagcac ctcgttgagc accgcgtgca ggtagtggcg cgtcagcccg ctcaggaaat
    16621 ccgccggcgc gagcgtcagg tgcgcggcgt ggctgtggcc acgcgcgaga gccggcggct
    16681 cgcccagcgg cagcagccgg cgcaggcgga cctcgcgcgt ggcatcgcag tggtacacga
    16741 ccgacaggct gctggcgccc ggcgcggctc gcgcgagcag ggtggcgagc tcgcgggtca
    16801 ggcgcagcag caccgcgggc acttcgtccg ccacgatcgc gccgggcagc accaggtcga
    16861 cgtcctcgcg ctcgccacaa cgcccggcca gccgctgccc caccacgacc cggcgtggcg
    16921 gcgccccccg ggagcccagc aagcgctgcg aggcgctcag cagcgcctcg ttgaagtcgc
    16981 cgcaaaagcc ctgctccgag ccgaccagca cgcacaaccc gccaccgtgc gcggccggcg
    17041 cgccgcccgc cccggcgtgc caggccagga agtcgccggc cgccgcctcg atgcccgcga
    17101 ccatgcggcg ctggctgccg aggaactcct gcaggatgcg cgtctccatc agcgccaggc
    17161 ccttcatcgc ggacatgatg cccgcgatgt ccgccagcgc ctccatgcgc ctggagagct
    17221 cacgccggcg gctcatcgcc cgcctccgcg ccccaggcct cgatacaggc gcaccacgcc
    17281 tcgcgcccgg cgctgagccc cggccccgcg ctggccgcgc gcgcgagcag ccatgccagg
    17341 cggcgctgca cctgcgggcc cgcgcacgcg tcgaagaagc cctcgttgta ggccaccagc
    17401 cacgccagtt gctgctcggg accgagcggc gccaggcgtt cctgcttgag cagctcgcgc
    17461 agcagccggc cgcgacggat cttggcttcg acgcccgcct ccaggcgcga gccgaagcgc
    17521 gtgaacacct cgagctcgag gaactgcagg aaatccagct tcatgcggcc cgcctcggcc
    17581 ttgatcgccg gcagctgcgc cttgccgccg atgcgcgaca ccgagcgcgt gatgtcgatc
    17641 gccggcagca cgccgcgcga gtagagcgcc ggatcgaggt agacctggcc gtcggtgatc
    17701 gagatcaggt tggtggggat gtaggcggcg atctcgccct gctcggtttc cacgatcggc
    17761 agcgcggtca tgctgccgcc accgcagtgc gccgcgaggc aggtcgagcg ctccagcagc
    17821 cgcgaatgca ggtagaagac atcgccggga taggcctcgc gcccgggcgg gcggcgcagc
    17881 agcagcgaca gctcgcggta ggcgcgggcg tgcgtggtga ggtcgtcgta gaccaccagc
    17941 acgtcgcgtc cgccccacat ccattcctcg gcgagcgtgc agcccgcgaa gggcgccagg
    18001 aacttcaggc ccggcgtggt ggtggcctcg gcgaccacca cgcagctgat gtccagcacg
    18061 ccggcgcggc gcagggtctc cacggtcgcg accaccgccg agcgcttctg gctgatcagc
    18121 acatagacgc agagcacgtc gccgcccttc tgcgcgatga cggcatcgag cgcgagcgag
    18181 ctcttgccgg taccggcgtc gccgatgatc agctggcgct gtccgcgccc gatcgggatc
    18241 atcgtatcga ccatccggtt gccgctcagc agcgggcggg cgacgaaatc gcgcgcgatg
    18301 atcggcggtg atgccgcctc cagggcgcgc cggcggtgcg gccggggtgc gggcctgccg
    18361 tcgagcgggc gacccagcgg gtccaccacc cggccgagga agtcatcccc gacgccgatg
    18421 tcgaggcgcc ggcccgtcag gtgggccgag gcgccggcaa ccagcccgtc cttctggctg
    18481 agcaggatgg cgccgatgcg ccggctgccg aggtggaaca ccagcgcctc gctgccgtcg
    18541 ccaaagcgca ggatctcgtc catggccgcc gagggcagcc cctcgaccca gacgatgcca
    18601 tcgccgatcg ccacgacccg gcccatctcg ctgacgcgca gctgcgggcg gtaagccgcg
    18661 ggcgtcgcgc ggcgctccgc gccggcggcg ggtggcgcgc gctcagccgc caaggccgcc
    18721 gctctcggcg aaagcgccca gctcgccggc cacgctgaag tccagctgcc aggcaccgag
    18781 cgagaggcgc accccggcca gcagggtgct gtcctcgacg aagtcgaaat ccccgcgcat
    18841 gccgagccgc gcctcgacgg cagcggcgag ccgcgtgcgc agcgcctgcg gcagcgggaa
    18901 ggcggtggcg aagacgcccc gggcatgacc gttctgcccg gtgcgcaggg cgccgaggcg
    18961 ctcctcgggc agcgcgccca gctcctcgac gaagagctcg accaggcgag cctctgtctc
    19021 gcgcccggcg accctgccga gcagcgcgcg cgcgaagcgc cgcgcctggg cgccggcctc
    19081 ggccgccagt tcgcgccgca gcgcttcctg gcggtgcgcc tcctgggcgg cggtgcgctc
    19141 acgctcggcg gcaagggcct ccgcgagcgc ctccatctgc cgctcgcgct ccagcgccag
    19201 cgcggtttcc aggctcgcgc gcgcggcggc cttttcctgc tcccagtcgg ccaggcggct
    19261 ctcgaactgg cccttcagcg cgctggcgcg ctcctcggcc tccttcgcct cggcgagcac
    19321 gcgctcgatg ccggtcttgc gcgccgccag gatgttcagc accggccggt agagatagcg
    19381 ctgcagtatc cagaccagga ccaggaaatt gatgacctcc aggacgaagg tggtccaatc
    19441 cagctccatg agcggctcct gctactgcag caagtattcg agcagcgggt tgcggaagat
    19501 gatcaccagc acgattacca gcacatagat cgccagcgat tcgatcatcg ccaggccgat
    19561 gaacagcgtg cgcgtgatgg atttttccgc ctccggctgg cgcgccagcg cctccagcgc
    19621 ctgggcgatg gcgcggccca tcgccatcgc cgggaacatg gtgcccagcg cgatcgcgat
    19681 ggcggccacc gcggtcgaga ccagggcaaa ccagtgtatg tcgctcatgt cgtgcctcct
    19741 ccggcatcgt tggaatcggc tgcggcgccg ccctgcgcca cttcccgtga ctgcatgccg
    19801 ccggcgatgt agatcagcgc cagcgtgccg aaaatgtagg cctggaccag tgcctcgacg
    19861 atgtgcagca tcagcaccgg caccggcacc aggaagcccg ccagcaacaa taccatcagg
    19921 gccgccattt ccaggctcat gatgttgccg aacagccgca cggccagcgc gagggtgcgc
    19981 gagagctcgc cgagcaggtg gaacggcagc aatatcgggc tcggactcag gtagtggcgc
    20041 gcgtagtcct tcagccccac gctgcggatg ccgtaccagt gcgtcgagag gaataccagg
    20101 aaggccagcg cggcggtggc ggaaagatcg ccggtcggcg aatcgagctc gggcaccagg
    20161 ccggtgaggt tggccaccac gaggaacagc cagatcgtgc cgacgaaggg cagcaacgtt
    20221 gccgcgcgcc cgggcagcac gctgtcgatc gcggactcga tcgcctgcac cacgccctcg
    20281 agcgcggtct gcagcagtcc ggggcgctcc acgcagagcc ggcgcgtggc cagccggcac
    20341 acgatccaga acagcagcag cagtccccag gtggtcacca ccgtggtggt gatgccgaaa
    20401 cctccgaggc tgaagaccac ggccacgctt tccatcacca gtacttccgg atgaagacat
    20461 agacgttggt ggctccgagc accagcccca gcaggatgca gttcagcgtc cagcgcacgg
    20521 tgtagtcctc ccccaggctg tccagccagc gcccgaggta ggctcccgcg agcaggggtg
    20581 ccacgaacag caccgacagg ctgcccagga acaccgactg cgccagcagc gagcggctac
    20641 cgcgctcggc gcgccgcatc cgctcgctgt cgcgctcgac accgcggcgc cactgctcgt
    20701 cgctcatgcc tgccaccggt cgagctggcg caggcgccgc agcagctgct gctccaggtg
    20761 ctgcacgttc tgccgcaggc tgcgcaactc ctcttcctcc cgggccagct ggcccgacag
    20821 cagccccgag atacggtcgt agtcgtggtc acggaggtat cggcgcgtgt tcaccgtgag
    20881 ccggtccgcg gcaaagtaca gcaccgcgcc gggacaggcg agatgacacc agcgcccgtc
    20941 ggcgtcgcgg tagcgcgaca gcccgtagcc gaggatggtc atgaagcgct cgcacccggg
    21001 caggatgccg aaactgccgc tcgcgtcggt gcccacgaag ctggtgacgc cggcaatgtc
    21061 ctcgctgtgc acggcgctct gcaggcacag cgtgatggtt gcgctcacgg ggcctccatc
    21121 gcgccgcgca tgtagcagcg ctcttcgggc atcgcgtcat agcgcccgcg catgaagtcc
    21181 tcgcagtcgc cgagcgtgac cgcgagcggc accgaggcgc ccgcgatgcc ggtgtgctcc
    21241 gacaccacga agaatggctg cgtcaggtag cgctcgagca ggcgcgcgcg cagcacgatg
    21301 cgccggtccg tctccgacag cgcctccagc cccagcatcg cgatgatgtc ctcgagctcg
    21361 cggtagcgcg cgaggtgctc gcgcacgccc tgcgccacgc ggtagtgccg ctcgccgagc
    21421 gtcacccggt ccatcaggcg gctgccggat gccagcggat ccaccgcggg atagatgccc
    21481 ttggcggcct ggttgcgcgc caggatcacg gtggtgtcga ggtgcgcgag gatcgcgccg
    21541 acggccggat cggtcatgtc gtcggccggc acgtagaccg cctcgaccgc ggtgatgctg
    21601 ccgcggctcg tcgaggaaat gcgctcctgc agttccgcca cctccgacag cagtgtgggc
    21661 tggtagccca cggtggcggg catgcgcccg agcagtcccg agagctcgct gcccgcctgg
    21721 atgaagcgaa aggcgttgtc catcaggaac aacacgtcgc gcgccacgtg atcgcgcagg
    21781 tactcggcat aggtcagcgc cgacaggccg atgcgaaagc gcacgcccgg cgattcgtcc
    21841 atctggccga agaccatcag cgtgcgcggc atcacgccgg cgtcctgcat gtcgtgccag
    21901 agctcgtggc cctcgcggat gcgctcgccc accccggcga agaccgacac cccgcggtgc
    21961 aggtgcacga tggcgtgcat gaactccatg accagcacgg tcttgcccac gcccgcgccg
    22021 ccgaagaggc ccgtcttgcc gccgcgcacg aacgggcaca gcaggtctat caccttgatg
    22081 ccggtcggca ggatctcgcc gacgccgatc gcctcgtcga gcggccccgg ggccgcgacg
    22141 atcgggcgcg tgccgctggc ggcgagcggc gcagcgccgt cgagcgggtt gccgaacaga
    22201 tcgagcaggc gtccgagaca gtcgggcgtg accggcaccg cgagcggccc gccgctgtcg
    22261 aagaccggca ggtggcgacg cagccccgag gtgccgtgca gcgcgacggc gcgggcgcgc
    22321 gattcgtcga ggtggcggtg cacttccagc gggtaacgtt cgccgtcggc gtgcacgtag
    22381 agcgcctggt gcagcggcgg cagccgcgtg cacaggatgt cgatcaccgg cccgtggact
    22441 tcctcgatga cgccgagcgg ctccggtgcc cggagttcgt ccgcgggtgg cgcggcggag
    22501 acgaagggcg tgtccatggc tcagcgtcgc cgcacgcgct cgcggaaata gcgcggctcg
    22561 gtatcgatgc cgagcagttc gcgacgcagc aggtccaggg cagtcgcggc gaccatggtc
    22621 tggaacagct tgcgcgcgac cgggaaccag agctcgcgcg cctgcacgcg ccgctcgctg
    22681 ccccaggcga tccacaccgt gccaaccggc ttgtcgggcg tgccgccgtc ggggccggcg
    22741 atgccggaaa ccgcgaccgc gtagtccgcg ccgctgcgcg ccagcgcgcc ctcggccatc
    22801 gcgcgcacca cggcctcgct gaccgcgccg tggctggcga ggctagcgcc ggcaacgccg
    22861 agcacgctct ccttcatgcg gttcgagtag gtcacgaaac cggcgtcgaa gacctgcgag
    22921 gcgccgggga tctcggtgag cagcgcggcg atcaggccgc cggtgcagga ctccgcggtc
    22981 gcgagccgct tgccggcggc agcggccagc tccacgacgc gccgctgcag gctggtgtcc
    23041 ccggttccga cgatgtgctc ggcgaacagc gcctgcaggc gctgctcgca ggccacgcgg
    23101 tcggcctcgt cggcgctgcg aaaactggag accttcagct ccagcgtcgg cgccccggcg
    23161 cgaaacccca gctcgatgtg cgcgggccag tcggggaatt cctccgtgat gcgctgctgc
    23221 agcgacgact cgccgatgcc gaacagctgc atgcgcgtga tcaccacgcg ttcgctcgag
    23281 ggaaactcgg cggccagcca gggcacgatc tcgtcgtcga gcatctggcg cagttccgag
    23341 ggcacgccgg gcgtgcacac gatctcgcag ccgcggtggc gcaggcgaaa gcccaccgcg
    23401 ctgccgaccg cgttggcgac gatcgtcgcg cccgccggca gcagcgcctg tttgcggttg
    23461 gcggcgttca gcgccagccc gcgccggccg caccacgcct ccaggtgcgc gagcgccgcc
    23521 ggctgctcct gcaacgggac gcccgccacc ctggccagcg cctcggcggt gaggtcgtcg
    23581 ctggtgggcc ccagaccgcc gttgacgatc agcacgtcgg cgagccccgc gagccgctcg
    23641 agctcggcgc acagcagcgc gaaatcatcg ccgatcgtga ccttggcggc gacctggcga
    23701 ttgagctgcg cgagcttctg cgcgatcagc gaggagttgg aatccagcgt gaccccgctc
    23761 atcagctcgt tgcccgtcat cagcagtagc agtctcacgc gctggcctcc ccgcgcccgt
    23821 gccggcgatg tacgcgatcg atggtcgatc cgtgcgcagt gtaatccatc gctgccgcag
    23881 cgcgctcagc gcggcgcaag cgccgcttcg cgcccgggca gcgcgccgag cagcaccagc
    23941 gccagcgcgg cgcacgcagc accggcggca taggccgcgc ccgcgccgaa gcgcgtccac
    24001 agccagccgc ccagcacgcc ggcgagcacg gtcgccagcc ccgttcccag gtgcagccag
    24061 ccgaaggccg cgccgcgcgc atccgccggc gcgcgatcgg ccagcaacgc cgccagcagg
    24121 ccctgcgtgg cgcccaggtg cagcccccac accatcgccc cgagcagcgc cgtccacgga
    24181 tcggcggcgc gcgcgagcag caggtcggcg agcagcagca ggcccagccc gcagaccagc
    24241 acgggcttgc gccccaagcg gtccgagcac gcgccggcgg gccaggcgct gagcgcatag
    24301 gccacgttca tcacgatcag caccagcggc accagcgccg tggccagccc gagctcgctc
    24361 gcgcgcagca gcacaaaggc ctccgccagg cgcgcgagag ccagcagcac ggccagcgcg
    24421 atcaccgcgc gacaaccggg tggcacggca ccgggtcgca gccaggcgaa cgtgaagcgc
    24481 tgcgtcgcgg cgcgcgggcg cggcacggcc ggctcgcgca cgaacaccac gagcaccgcg
    24541 acggcgagca gcgccggcac ggtggcgacc cagaacaccg cgcgcacatc attcgccagc
    24601 aggtacatca gtgcgatcgc gagcgccggc cccagcaccg cgcccaccgt gtccagcgcc
    24661 tggcgcagcc cgtaggcggc accgcgcgag gcctgcggcg tgacgtcggc caccagggca
    24721 tcgcgcggcg cgccgcgtat acccttgccg aggcggtcga tcgcgcgcgc ggcgaacacc
    24781 gtcgcggccg aaccggccag cggaaacagc ggcttgctga gcgccgccag cgcataaccg
    24841 cacaggatca gcggcttgcg ccgcccgctg cggtcgctga gcgcgcccga gaacacgcgc
    24901 atcacgctgg ccacgccttc ggccgcgcct tcgatcaggc cgagcacgag cgggctggcg
    24961 ccgagccccg ccaccaggaa caccggcagc aggctgtgga tcagttcgga ggatgtgtcc
    25021 atgcacaggc tgacgaagcc cagcgcgacc accgtacgcg gcacaccctg ctgcaattgt
    25081 tgtccctccc gtcaggcgca cccgcacccg atcccggcta gactgcccgc tcgacgcccg
    25141 agatcgcaag ccatgagcgc actccgcccc gcaaaccatc gcgaccgccc cgctgcaagg
    25201 cagcgggcgg cgcgcgccga ttgcccggca aggtactgag atgctcggtt atcgccacgc
    25261 cttccatgcc ggcaaccacg ccgacgtgct caagcacgtg gtgctgctgc agctgctcga
    25321 ctacctggcc gccaaggaca agccgttctg gtacatcgac acgcacgccg gcgccgggcg
    25381 ctacacgctg acgcagggct acgccgccga gcagcgcgag tacgtcgacg gcatcgaaaa
    25441 gctctggaac gcgggcgtgc tcccggaagc gctggcacgc tacgtcgagc tcgtgcgcac
    25501 gctgaacccg caccaccggc tcaagtacta ccccggctcg ccgtggatcg cgcgcgcgcg
    25561 catgcgccct ccggaccgcc tgtggctgca cgagctgcac ccggcggatt acgcattgct
    25621 ggagcaggaa ttcgccgccg gccccgtgcc cacgcgcgtg ttcagcgagg acggctacgc
    25681 cgggctcaag gcgctgctgc cgccggagcc gcgacgcgcg ctggtgctga tcgacccgtc
    25741 ctacgaggtg cgcgaggact acgcgcgcct ggtcgaggcg ctgaagcagg cgctgcgccg
    25801 cttcgctacc ggcgtctaca tggtgtggta cccgatgatc ccgcgccgcg agtcgcgcga
    25861 gctgcccgcg cggctcgcgg cgctggctcc cgaacgctgg ctgcgcgccg agctggtggt
    25921 gcgcgcggcg gacgccgcgc acggcctgta cggcagcggg gtgttcgtgg tcaatccgcc
    25981 cttcacactg cgcgccgccc tcgcggagag cctgccgcgg ctgacgatgc tgctggggct
    26041 cgaccgccac gcgacgttca cgctcgaggg cagcgccgac taggcgcccg cgcggcacgc
    26101 acgccggagc ggtagcctca agttcgcgcc gccgcggccg ataagccctg ctgcagtacc
    26161 agccggggtg acgatgaaga tccagggttc gcaggtcgag ttgcgcgcgg ctcacttcga
    26221 gaagcgcgag caccgcatcg agcggcagtt gcaggtgtcc atcacgcgcg cgcccccgcc
    26281 acccgccgcc gacgccgacg ccgccgctcc cgcggcatcc gccacgtccg cgctcgacgc
    26341 ggaggtccgt tacttccagt tgctgataga aatgctctcg ggccgcgcgg tccgcatcgc
    26401 gaccatcgcc agcgacgcct cggcgacagg ggagcctgcc gacggggcgc ccgccgcctc
    26461 gccggccccg tcgctccaga tcgcggtctc gcagcgcacc agcgaacatt acagcgaggc
    26521 cgaggccagc gccttcgcgg ccgaggccac cgtgcagacc gccgacgggc gcgagatgcg
    26581 cctggagctg tcactgctga tgcagcggcg cttcgagagc acgacgtcgg tcacggtctc
    26641 gtccggcgag cgcaaggacc cgctgctgct caatctcgcg ggcaatgcgc ccgagctcgg
    26701 tgccgtgaaa catgccttcg acctcgacgc cgacggcacg caggaaatgg ttgccatggc
    26761 cacgggccgc agcgccttcg tcgcgctcga tcgcgacggc gacgggcgaa tcaatgacgg
    26821 ccgcgagctc ttcggcgcgt tgaccggcga cggtttcgcg gagctagcgc gccatgacga
    26881 cgacggcaac ggcttcatcg acgcgggtga cgcggtgtac gcgcgcctgc tggccttcgg
    26941 caaggacgcc cggggcgctg atgtgctcac gccgctttcc gcgctcggcg tcggcgcgct
    27001 gtacctcggc agcgtggcaa cgcccttcag cgtgaacacc gccgcgaacg agacactcgc
    27061 cgtggtacgc agcaccggcc tgtggatcgg cgaggatctc tccgcgggaa ccctgcagca
    27121 gctcgacctg gtcgtctgag cggcgcgcca ctcagggcgc gagccgctcg atgcgccact
    27181 gcccgtcgtc ggcgagcgtg tagctgaagc ggtcgtgcag gcggttgggg cggccctgcc
    27241 agaactcgaa ggcatccggc accacgcgaa agccgcccca gaacgagggc agcgggatgc
    27301 ggccctcgcc gaacttctgc ttcaggcgcg cgaactcgtc ctcgagcagc gcgcgcgagg
    27361 acagcggacg gctctgcgcc gaggcccacg cggcgagctg gctggcgcgc gggcgcagca
    27421 ggaaatactt cagcgcctgc gcggcgccca tgcgctcgac cgtgccctcg atgcgcacct
    27481 gccgctccat gtccgcccac gggaacagca gcgccacccg cgggttgccg gcaatctcgc
    27541 gcgccttgcg gctctcgagg ttggtgtaga acacgaagcc cgcgtcgtcg cagtgcttca
    27601 gcagcaccag ccgttgcgag gggcgtccgc gcgcatcgac ggtcgccacc gccatcgcgg
    27661 tgggatcgcg caggcccgcg tcgatcgcgt gtttcagcca gctctcgaac tggcggaacg
    27721 gcgaggcgtc gaggtccttg cggtgcaggc cgggagcgag gtactcgcga cggaaatctt
    27781 ccaggtccat gggcggcgtt ccggcttttc ggtgatgaac ggagcgcgag attagcagat
    27841 gccctgcgct ctcaggtgct gagcatcgtg gcgagaaatc cggagatcac gatgccgtcg
    27901 aaagtgccgg cgccgccgat gctcatcagc ccgcccccgc gccggccgag ctcgcgcagg
    27961 tgcagcaggt cggcgccgat cagcggcccc agcacgccgg cgcagaaagc gaccggcgcc
    28021 gcgtgctcgc ccgccagcag catcgcgacc agcgcggcga gacaacccgg caccagcgcg
    28081 ggcagcagga tgccgagtcc cgcgaccggt cgcgccatca ggtagcaggc cgcgacattc
    28141 agcgcgatcg ccaccgcggc gtgcagcggg gcgccaccac cggcgtcgag caggcgcgcc
    28201 agctcgtaga ccacgatgag agaaggtatc aggcagccgc cgacgttcag cgctatgacc
    28261 tgtggggcac cgggctgaac cagccgcggc gcgagccggc caaggccgaa gaaatcggac
    28321 gggcggtatt ccagcaccgc cgcgccggca cggcgccata ccggcacatt gaacaggctg
    28381 ccgacgaaga tgccgatcac cagcagcgtg gccacgcgcg gcgcgatgcc tagcccgagc
    28441 agcgccgaca ccatcgcatc ggcgagcagg aacggcagca gcagcaacat cgccagcgcc
    28501 acgatcagcg caagcactcc ggcgggcatc gagggtcctc ctgtcgccgg gcgtcgcgac
    28561 atcgatgaac cgccccgggc gtggcgcgac tctagcgcag cgcgcccgct taatgaagct
    28621 gtcacagatc gcccggagca tgtgccgcga acaagggagg agacacctcg catgcaaacc
    28681 gataccggca tcgacgtgaa aaacctgtcg gaccgcaggc tgtgggagat cctgcgcctc
    28741 gccgagaccg acgggcagcg cgccgccacg gcactgatcg cccacgcgcg cgacgaactg
    28801 agcgtgcgcc gccagcacac cgagacgcgg cgctttcgcg cgcctcactg agcggctcgc
    28861 cggcgtcatc aaaaattcac cgaaacacca cgagacctca acaatcgccg cgcatcctcc
    28921 gtgcatggaa cgcgcagggg atacggcatt gctgcagaac atccaccgcc tcgacctcct
    28981 tgccttcggc tggtgccacc agcgcaacgc cgaagccatg atcttcgtcg cggcgcgccg
    29041 gatctcgcgc agcgccgacg gctggctcta cctgctgctg ccgctgctgt tcctggccag
    29101 cgacacgatc gacgccgtgg gcttcatcgc gctgggctgc caggccttcg ccctcgagcg
    29161 cagcgcctac ctgctgatga agaacacctg ccggcgcagg cgcccgcccg atatcctgcc
    29221 cggcttccgc agcctggtga tcgcctccga ccgtttcagc tttccctccg gccacacctc
    29281 ggcggcgttc ctgttcgccg gactgtgcac ccaggagttc ggcctctggg cggctgtgct
    29341 gctgtatccg tgggcctgct gcgtgggtgc ctcgcgcgtg ttcctcggcg tgcactttcc
    29401 cagcgacacc gtcgccggcg cgctgctcgg cagcgcgctg ctgctgggag cgcgcacgct
    29461 gttcaccgca tgaagatctt ctatggcgtg cagggcacgg gcaacggtca cctgacgcgg
    29521 gcgcgggcaa tggcgcgggc gctggcacgc tacccgcagg tgcaggtcga ctggctgttc
    29581 accgggcgcg agcgcggcga tttcttcgac atgcaatgct tcggcgcctg gcaatggcgg
    29641 cgcggactga gcttcgcgac ccgcgcggga cgcgtgcgcc cgctcgcgac gctgcgccag
    29701 ctctcgcccg gggagttcct gcgcgacgtg cgcgagctcg acctcgaggg ctacgaccgc
    29761 gtggtttccg acttcgagcc cgtcacggcc tgggccgcgc gccggcaacg gcgcgactgc
    29821 atcggcctgg ggcaccagta cgcgctggtg cccggtgtgc cgcgctcgcg gcgccacctc
    29881 gtgggcagcg tggtgctgcg ggcctgcgcg cccgtgggca cgcgcatcgg gctgcactgg
    29941 caccacttcg ggcacccgct gctgccgccg atcgtcgacg acgacgcgcg cccggcggac
    30001 gtgccagcgc gcgcctcagc gcgcgaagcg ctggtgtacc tgccgttcga ggatccggag
    30061 cgcgtgatcg cacttctgcg cgcgatcccg ggctggcgct tctcgatgtt cgggcccgga
    30121 ctgccccggg agcagcgcgg caacgtgacg acgcacccgg tggggcgcgc cacgttcgtt
    30181 gcggcgctgg agcgtgcgag ccatatcgtg tgcaactgcg gcttcgagct gatcagcgag
    30241 gcgctgtgcc atggcaagcg catcctcgcc aagccgctgg ccggccagat ggaacaggtg
    30301 tccaatgcgc tggcgctgca ggcgctgggc tacgcgcgga tcgcgcaacg actcgacccg
    30361 cagctgatcg gcgatttcct cgcgctcggg acaaccgcgc cacggtcgga ctggccggat
    30421 gtcgcggcag cgatcgccac gtggctggtc gaaggcgggg agccgctgga agcgctgagc
    30481 gagcgcctgt ggcgcgagtg cgcaatcgcc gagccgcgcg ccggcagcga cgcgagcgac
    30541 cgccccgcgg cgctgcgcgt cccggcctga gcggatcagc gggccatcgt gagcggcagg
    30601 tcctgcaccc aggccaggcg caactcgcgc gaggcgaagc gcagtccgcc gtcgtgacgc
    30661 acgatgcggt cctggtagcg gatcgccatg tcgagcttcc attcggcgcc gtcacgctgg
    30721 tagacatgcg aggcgatgca ataggtctcg ccgctccaca cgccatcatc gccccacgcg
    30781 ccgagctggt tgccgaccat gtgaaaggtg cggtcgtact ggcgcagcaa ttccatgcca
    30841 cgctccacct cgggcagggt atccatcacg taaccggggc ccgtgatgcg gatcgagggc
    30901 agcatgatcg tgtgcaactc gtccaggcga cggtcgtcga tcagtgcggc atagcggcag
    30961 gcgagatcca gggtgtcgtt gctcattggt gcggctcggc ggaagttcgc ggcaagttat
    31021 cgcggcatgc cgctgaaagc aaaccgggcc tcgcgcgcgc aaacaaaaaa gggccgtcgc
    31081 tcgccacgac ggcccttctg tgaattggtc ggagtgaaag gattcgaacc ttcgacccct
    31141 gcctcccgaa ggcagtgctc taccaggctg agctacactc cgaatacgtt cagttgccgc
    31201 gcgggcctgt ccacgcggcg cgcattgtaa tggaaagttc cctcagggga tatacattcg
    31261 ttcgatgatg atgctcagca ccgtcatcgc ggtcgtggcg acgaggatca cggccagcag
    31321 caccagcggg cggaagggct tgcgcgggac ggagtgaatc cccgtcgacg tgaaggcatc
    31381 gacccgctgc tggtcttcgg gatggagttt tcctgcttcg ctcacgatgt ttcatcctgc
    31441 gccgcggcgg cggcgccagt cattggggag agcagtataa gcgcgcgggg ccgggcgcga
    31501 gaagccgcgc cctcgcccgc gccgcgttca cagcgcgaaa tgcgcctcgg gcaaggccat
    31561 cagcgtggta ccgcccgcct cgacgcacag cgccaggctc tcggtgcgcg gcaggatgcg
    31621 ctggaagtag aagcgcgccg tctcgatctt ggcggtatag aaatccgcct cgccggtgcc
    31681 gcccgccagc gcgtcgagcg cggtgcgtgc catgcgcgcc cacatgaagc cgagcacgaa
    31741 ataccccatg aacatcaggt actcgaaggc cgcggcatcg gcctcgtcgg gatttttcat
    31801 cgccgcgccc gcgatgtgca tcgtcaggcc ctcgagccgc ttgccggttg ccgtcagcgc
    31861 ggcgaccagc ggcgccatgt ccgcgttccc cgcgttttcg ctgcagaact gctcgacgat
    31921 ggcggcaaag ccgtgcagca gttcgccgcc gctgcccatg atcttgcgcc cgagcaggtc
    31981 cagtgcctgg atcgcgctgg tgccttcgta gatcagcgcg atgcgcgtgt cacgcacgat
    32041 ctgctccatg ccgtgctcgc gcacgtagcc atgcccgccg aacacctgca cgccgagatt
    32101 cgccgcttcg tagccggcct cggtgacgaa ggccttggcc accggtgtca gcagcgccat
    32161 caggtcctcg gcccgcttgc gctccgccgc atcggcagac ttgtccgcga tgtcgaccag
    32221 ctggcacagc cagtacagga aggcgcggct gccctcggca taggccttct gcgtgagcag
    32281 catgcgacgg atgttcgggt gcacgatcag cggatcggcg ggcccggagg ggttcttcgg
    32341 tcccgagagc gagcggccct gcaggcgctc gcgcgcatag gccagcgcgc cctggaacga
    32401 gagctcggcc gccgccaggc cctgcatcgc ggtaccgagg cgcgccgcgt tcatgaacgt
    32461 gaacatgtgg ttgaggccgc ggttcggcgc gccgatcagg aaacccgtgg cggcatcgaa
    32521 gttcatcacg caggtcgcgg aagcgttgat gcccatcttg tgctcgatgg agccgcagga
    32581 aacgctgttg ggtaccagcg tgccgtcggc ctgcggcagc ttcttgggca ccaggaacag
    32641 cgagatgccc ttggtgccgg gcggcgcgtc cggcaggcgc gcgagcacga tatgcacgat
    32701 gttcgcggcc atgtcgtggt cgcccgcgga aatgaagatc ttggtgccgg tgatgcggta
    32761 ggagccgtcg gcctgcggct cggcgcgcgt gcgcagcaag cccagatcgc tgcccgcgtg
    32821 cgcctcggtc aggcacatgg tgccggtcca ctcgccgctc accaggtgac gcaggtaggt
    32881 ctgcttctgc tcgtcggtgc cgtgcacctt caacgtccgg gtggccccgt gggagagccc
    32941 cggatacatc gtccaggccc agttcgccgt gcccatcatg tcctcgacga acaggcccat
    33001 cgagggcggc aggccctggc cgccgtattc cggcggagtg ctcagcgcag cccagccgcc
    33061 ttccacgtat tgcgcgtagg cctcgcggaa accggcaggg gtgcgcacgc cctcgggagt
    33121 ccaggtgcag ccttcctcgt cgccgctgcg gtacagcggc gccagcacgt tctcgcagaa
    33181 cctggccgcc tcgccgagga tcgcatcgat caggtcgggc gtgaactcct cgcagccggg
    33241 tagcgcggcg aacagtgccg gaaaaccgag caattcgtcc ctgacgaaac tcatatcgcg
    33301 cagcggggcc ttgtaggaca gcatgaagaa cctctggagc gggaattcga ccgcccggat
    33361 gctagtcgcg ggggcgcgca ggaaccatta catggcaggt aaacgcgcag gcctcaggcc
    33421 aggacgtcga gcagcgtgcc cagcgtgtcg tcggccgcgc gcaccacctg cgctgccgca
    33481 ccggcctgca gcttcgcggc ctgcagttcg acgatagcct cggtgagttg tccggcatcg
    33541 ccgcccgcgg cgctcgagac gacgcgcgtc gcggcggcct gggcgcgctc gagactgtcg
    33601 cgcagcccct ggaatccggt ctggccaatg ggagtgctgc tcacggggag tgctccgcga
    33661 aagtgaaggc gctgcaagtg tacatccgga cccgcaagcc tgccgtgcgc gggcactcaa
    33721 tcctgcgcgc cacggcgcag ccggccgatg tcgagcaaac ccgccgcagt acgcacgtcg
    33781 ataccggcgc gccacgggat gaaaccgatg cgcggggcac cgagcatcgc atcgagggtg
    33841 gcgagattgt cggcaagatt ggccatgccg ggatcgacct ggttggccac ccacccggcc
    33901 agccgcagcc cgtcggcgcg caccgcctcg gccgtcagca gcgcgtggtt gatgcagccc
    33961 agccgcaggc cgaccaccag caccaccggg aaacccagcc ggcgcgcgag gtcggccagg
    34021 gtctcggcat gattgagcgg cacccgccag ccgccggcgc cctcgacgac ggcgacgtcg
    34081 tgcggccgcg ccagcgccgg caggcagcgc gcgtgcagcg cggcaacgtc gagcgccagg
    34141 cccgcctcgg cgagggcgat atgcggcgcc accgcgggcg ccagcaccac cgggttcacc
    34201 agcgcgtagg gcagctcctg gttcatggcg gcgagcagcg cgcacgcgtc ctcgttgcgc
    34261 agcccctcgg cggtggcgac ggcaccggag gcgatgggct tgaggccgat cgtgcgcgcg
    34321 ccactcgctc cggccgcccg cagcaacgcg gccgcgacca gggtcttgcc gaccccggtg
    34381 tcggtgccgg tcacgaacca ggcctcagcc acggggtttc tccagccgca ggcacagcgc
    34441 ccggtaggtg gctggcaggg tgccgtcggg gttggcgaac aatgcgtagg ccgactgcag
    34501 ccggcgccac gcaccgcgcc cggcgagccc gccggggcga ccggcgttca cgttgtgcgc
    34561 cccgatcgcg cgcaactcgc gcgcgaggcc ctgcacgtcc gggtacgcgg gcaggtggta
    34621 cttctgcccg atcacgcgca cggcgagccc cgccgcgcgc gccgcccgtt ccagcgccgc
    34681 gacgctctcg aaacggttca cgtgcacgcc gtcgtcgacg gcgcgccaag cggcgcgcag
    34741 ttcccacagc gtgtcatctc ccagcgtcgc gatcagcgcc tggccgcccg gcgccagcac
    34801 gcgcgcgatt tccgccagcg cccgtaccgg gctctcgcac cactgcagcg cgaggctgga
    34861 atacacgaga tcgatactcg cgccggcgag cggcagctgc tcggcgtcgg cgcacagcca
    34921 actcgttgcg ccgcccggcg cgcctgcgcg agcctggcgc aacatgccct cggcaaggtc
    34981 gagcccgatc accccggcgc gcggaaaacg ctgcgccagc cgggcgctga aatggccggt
    35041 gccgctgccg atgtcgagca cgcgcgccgg gtcgagcggc ggcgccagcg ccagcagctc
    35101 ctcgccgatg gtgcgctgca gtgccgcggc gcgctcgtag cccgccgccg cgcggccgaa
    35161 ggagcgcgcg acatcctgct tgctgcgcgc ggcagcggcg agcggcgtgg cgaaacatgt
    35221 cagaagaaaa tccgtcaccc gcgcggcaac cgccgcgggc cgcgacagga acggtgcatg
    35281 tgccgcgccg ggcaccagcc acacgctggc gcgcggttgc agccgcgcat agtgcgcggc
    35341 cacctgcggc gccacgatgg cgtcgcgctc gcccagcacg tgcagcaccg gcagctccag
    35401 cgccgccagc ccggcgcgca ggtcgaggcg cgcgagcgta tccagtgctg ccagcaactc
    35461 gccggggtcg gcgggcgccg cggcgagcgc gcgcagctcg cgcgccatct cgcgctggcg
    35521 caggtcccgg tgcgcctgca gtgccgcgaa acgtgccagt tgcgccggcg cgtccgcgag
    35581 caggccggcg cgaaatgccg cgaagctcgc cgcatccatg cccgcggacc agccctcgcg
    35641 ctgcaggaaa caggggctcg tcgcgatggt gaccagcgcg gcgagatgac ccgggaaggc
    35701 ctgcgcgaaa cgcagcccga gcgcgccgcc gagcgaccat cccgccaaca cggccccggc
    35761 gggaatgcgc tcgcgcatcc agccgagcag tgccgcctcc tcgcgcggaa aggcctcgtg
    35821 cgcggccgcg ccgtgcgcgg gcagatccag cgcgagcaca tcgaaatgcg cggcgagcgc
    35881 ccgggccagg gcggcgaaaa cctcgccccg gctgccccag ccgtgcagca gcacgacgcg
    35941 acggcgcacc acttgccgct gcggcgcggg caccgtgggg gcagcggcgc tcatgcgccc
    36001 gcgatgtccg ccagcgcctg caacaggcgc tcgacctcgg cgacggtgtg cgcggcgctc
    36061 agcgtcaccc gcaggcgcga ctggccccgc ggcacggtgg gcggacggat cgccggcacc
    36121 agcaggccgc gcgccagcag ctgttcggac cagcgcagcg cggtggcggc atcgcccagc
    36181 agcagtggct ggatcgcggt ttgcgacggc agcaactgca ggcccagcgc attcgctccg
    36241 gcacgccagc gcccgatcaa ttcctgcagg tgcgcgcgcc gccacggctc ctcgcgcacg
    36301 atgcgcaggc tttcggcggc cgccacggcc agcgccggcg gcatcgcggt ggtgtagacg
    36361 taggtgcgcg cgaactggat cagcgtctcg atcagctcct cgctgccggc gacgaaggcc
    36421 ccggcgacgc cgagcgcctt gccgagcgtc gccatcagca gcggtacccc ggcgctgccc
    36481 atgccctgct tttccagcgt cccggcgccg cgctcaccga gcacgccgaa tccgtgcgca
    36541 tcgtcgacgt acaacaacgc atcgtggcgc gcacacaccg cggccagcgc cgccagcggg
    36601 gcctcgtcac cgtccatgct gaacacgccg tccaccgcga ccagcttgcg ccgcgcgggg
    36661 ctgcgcgcaa ggcgcgcctc gaggctggcg gcatcgttgt gcaggaagcg ctgcaggcgc
    36721 gcgccgctca gcagtccgcc gtccagcagc gaggcgtggt tcaggcggtc ctggaacacc
    36781 gcgtcgccgc ggccgagcag cgcgctgatc acgccgaggt tggcggcata accgccggag
    36841 aacagcagcg cgcgcgggcg attcgtgaac tgcgccagcg cttcttccag ctcgtggtgc
    36901 gcgcggctgt gacccgagac caggtgcgag gcgccgctgc cggcgccgta gcgggcagcg
    36961 ccctgctgca gcgcaccgat cacgcgcgga tcgttcgcca gaccgaggta atcgttgctg
    37021 cagaaggcga gcagctcgcg gccatcgacg cggaccgtgg tggcctgcgg cccctcgagc
    37081 acgcggcgcc cgcgatacag gccggcggcc gccctggccg cgagatccgc gccgaggctc
    37141 gcgaaagcgc ccgccacgcc gcgcgcgctc aggccgtggc ggcgtcgtag aacagcgcgt
    37201 tcgcggccac cgggcgcgcc gcggcccgat caggggcggg cgcgcgcttt tgcgggcgca
    37261 ggccgaggcg gcggaacagc tgcatatcgc gctcgctttc gggattcgcg gtggtcagca
    37321 gcttctcgcc gtagaaaatc gagttggcgc cggcgaagaa ggccagcgcc tgcagctcgt
    37381 cgctcatgct ctcgcgcccg gctgacaggc gtacatgcga ggccggcata aggatgcgcg
    37441 ccaccgcgat cgcgcgcacg aactcgaagg gatccaggtc cggctgctcc tccagcggcg
    37501 tgccggggac gcgcaccagc aggttgatcg gcacgctctc gggatgctcg ggcatcgtgg
    37561 ccagcgcgag cagcaggccc gcgcggtcct cgcgcccctc gcccatgccg acgataccgc
    37621 cgcagcagac tttcatgccc gcctcgcgca cgttggcgag cgtgtcgagc cgctgctgga
    37681 agcagcgcgt cgagacgatc tccgggtaga actccggaga ggtgtcgatg ttgtggttgt
    37741 agtaatcgag cccggcctcg gccagcgccc cggcctgccc ggcgctgagc atgccgagcg
    37801 tcatgcaggt ctccatgccg agatccttga cgcccctgat catggccgtc agcaacggca
    37861 ggtcgcggtc cttcggcgag cgccacgcgg cacccatgca aaagcgcgag gaacccgcgg
    37921 cacgcgcggc gcgcgccgag tccagcacgc gctcgacctc gagcagcttc tcgggctcga
    37981 gcccggtgtc gtagcgcgtg ctctgcgggc agtacttgca gtcctctggg caggcgccgg
    38041 tcttgatcga gagcagcgtg ctcacctgca cctcggtcgg gtcgaaatgc gcgcggtgca
    38101 cgccgtgggc gcggaacagc agctcgttga acggcagcgc gaacagggcc tcgacctcgt
    38161 cgcgcgtcca gtcgctgcgc acggcgccgg cgttgcttgc attgggcatc acaaaccccg
    38221 aaagtggctc gaaaaggggc tggaagtgtc cggcccggcg aatagcttgt caaccggaac
    38281 ggacgccaat ggtttacaag tcactgctgc gcgggctgct gcccgccaca tgcctgctct
    38341 gtggtggcgc ggcccgcgca gcgctggacc tgtgcgccga ctgcgccgcg caactcccgc
    38401 tgctcgacaa tgcctgcccg cgctgcgcga tgccgctggc ggatgcgcac ggcgcgccct
    38461 gcggcccctg cctgcagcgc gcgccgcgct tcggcgcctg catcgccgcg ctgcgctacg
    38521 agcacccggt gcgcgaactg gtctcgcgct tcaagttcca gggcgatctt gcctgcggac
    38581 gcatgctcgc ggggctgctc gcacgacgcc tcgccgcact gccgccagcg cggcgaaccg
    38641 gtctggtgct ggtgccggtg ccgctgcacc cggcgcgcct gcgcgagcgc ggtttcaacc
    38701 agtccgaacg catcgcgcgc gtgctcgccg ccgaactcgg actcgcgctc tcgaacaccc
    38761 tggtgcggcg ggtgcagcgc accgccgacc agaagcagct gggcgcggcg gcacgcagcc
    38821 gcaacctgca gggtgccttt catgccacgg gctgcgccgg gcgacgcatc gcgctggtcg
    38881 atgacgtgat caccaccggg gccaccgccg aggcggtcac ggcggcgctg ttcgccgccg
    38941 gcgcggcgca ggtcgaggtg tggtgcctgg cgcgggcgct gtgagggagc gctgtcccga
    39001 tcgcaggggg gattccccgg ggaggcgggc agagccgctc gccccggctc gcgggcacgg
    39061 gcatcagaat tcccgggaag cgccgatgcc gtccacgggt tcgccggcag ccaggcgccg
    39121 ctggatgtcc tcg
//
LOCUS       DAORUQ010000008        38666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_644_length_38666_cov_6.321333, whole genome shotgun sequence.
ACCESSION   DAORUQ010000008 DAORUQ010000000
VERSION     DAORUQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 38666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38666
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_38666_cov_6.321333"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLW13_01530"
     CDS             <1..185
                     /locus_tag="PLW13_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69077.1"
                     /translation="LELDAIAFNIDAAFWPDPRRERARLAYRLDVNEYRGNRSVQLLV
                     EELQEPTVAQEMPCSA"
     gene            173..1111
                     /locus_tag="PLW13_01535"
     CDS             173..1111
                     /locus_tag="PLW13_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY69078.1"
                     /translation="MQRLSGRWQLGFWLALTTAVLWGAVPLAMTPLIAHLDAVTISWF
                     RFASSGLCLLAFFAARGRLQWPHSAGRTAAWLFALAVLMLIGNYVLYVQSLHFIAAPV
                     AQTVVQLAPVLLMLLSLWLFGERFTTWQWGGFVLLLGGITAFCAERLRVSGAGIDIFS
                     TGVGLMVLAAALWAVYGVAQKRLLRYLPSQFVLMGIYLASALLMLPAASPGALAALGA
                     TELGLLAFLAANTLIAYGAFAEALNHWESSKVSAVLAVQPLTTLFGAAALGALFPAHF
                     PALPLTAGAVVASFAVVAGSAICALGGREAQPGAAD"
     gene            1205..2300
                     /gene="prfB"
                     /locus_tag="PLW13_01540"
     CDS             join(1205..1276,1278..2300)
                     /gene="prfB"
                     /locus_tag="PLW13_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276406.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQY69079.1"
                     /translation="MEINALQNQLKDLGARALSLRGYLDYDVKKDRLKEVELELSEPS
                     VWDNAERAQELGRERAALAAIVDTLESLEGALGDSTELLEMAAAENDEQTFVSLEADV
                     QQLARQVETLEFRRMFSGEHDVANAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEAKGF
                     RTELIECSAGEVAGIKSATIHFQGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFAS
                     VFVSPEIDDAIEIEINPADLRVDTYRASGAGGQHVNRTDSAVRITHEPSGIVVQCQSQ
                     RSQHQNRDFAMKQLRAKLYEMEIQKRNAVAQALEDSKSDIGWGSQIRSYVLDQSRIKD
                     LRTGVETSNTQGVLDGDLDRFIEASLKSGL"
     gene            2320..3810
                     /gene="lysS"
                     /locus_tag="PLW13_01545"
     CDS             2320..3810
                     /gene="lysS"
                     /locus_tag="PLW13_01545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQY69080.1"
                     /translation="MTEHDSAQQDENRLIAERRAKLDAIRRERNAFPNDFRREHYAAD
                     LQRDYAGHSREQLEALGKRVSVAGRIMRMRGPFLVIADVGGQIQLYVNRETLPAATLA
                     EIKTWDIGDIVGASGVLHRSGKGDLYVNMDSARLLTKALRPLPDKYHGLADLETRYRQ
                     RYVDLIMNEDTRKLFRTRSVVLDALRDYLTDRDFLEVETPAMQVIPGGATARPFVTYH
                     NALDLEMYLRIAPELYLKRLVVGGFERVFEVARNFRNEGLSTRHNPEFTMIEFYMAYA
                     DYRDAMDITEGMLRQAALRGTGGTLVDYQGEQYDLGAPFARMSVFDSILHFNPTLTAE
                     AIASRDRARQVAEHLGIEVKPGYGLGKIQIEIFEKTVETRLRQPTFITGYPTEVSPLA
                     RCNDDDPFVTDRFELFIGGRELANGFSELNDPEDQAARFRDQVAMKEGGDNEAMHFDS
                     DYITALEYGMPPTAGVGIGIDRLVMLLTDSPSIRDVLLFPHMRPQA"
     gene            3816..4385
                     /locus_tag="PLW13_01550"
     CDS             3816..4385
                     /locus_tag="PLW13_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69081.1"
                     /translation="MRRGSVRTLLAPALLVALGGCQGMPTEAPLREPAPEAQSVSAPA
                     PAALPETTPGQVREDYDQVLRQASAAWERGDHRSALLLYERVLEGAAQPREQVRALIA
                     MATLRLMPSSKVTDSAAAAVVMRELDLRLAALDLRHEYYAQLELLRLLQAREGELQTL
                     RESNRKLAAQLAAKDELVRQLRALSVDGG"
     gene            complement(4352..6706)
                     /locus_tag="PLW13_01555"
     CDS             complement(4352..6706)
                     /locus_tag="PLW13_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY69082.1"
                     /translation="MSLLQRLTIAIFSVLLLFAVNVVSYSIGNRTIRDSLDAVSDAVQ
                     GQINASTVRQDMDDLHKQLLVLVTLQESTGQGISGPEAERTREAIAGLRQLIARMERN
                     TNEDSAAAHAALQAHAQTLFGDWENVLSQLRRQGTHGLSAAELKVAYAGFERALGDFE
                     AAVIAVSRAQSEHVESTGRTIGRVTVIIFLSSIFVTSLLGFLLIRHTNESLMRLREGT
                     VRIGGGDLDYRITVPSHDEFGQLAAAFNDMSEKLRAAVLEVQEAKEQADRANAAKSGF
                     LANMSHELRTPLNAIIGYSEMMVEMAAEEQDLPAAELSGDMQRILTAGRHLLSLITNV
                     LDLAKIETGKMTLYREPFDAIEVLRELGTTMHTLAAHNGNTIRIAATPHEAALVSDQT
                     RFRQIFANLISNACKFTTDGEITVDTEPFTREGADWLRFRVRDTGIGMTPAQLDSVFE
                     AFVQADSSTTKRYGGTGLGLSLCREFCQLLGGTIGVASEPDQGSVFTVELPVDATTVP
                     ELFFATAAPGAETAALPTAAPSGAQTVLVIDDDADARTISARVLRGEGCRVLEAESGD
                     EGLRLAREQRPDLIVLDLVMPGMDGWAVLSVLKDNEETRDIPVVLQSMLDARDEAMSK
                     GAADFLDKPVNRRRLAETLERLAPRDRSGHVLLIESESQAREELIAGLQGQGWYVSST
                     EQAGEALAIARQSLPDLILLSLGLPSEDVFALSEELGRSPLLSRVPVFVLAGADTHAD
                     AHARLASHLDRLVLHDAGSVESLLEQTGRIAAASQPPSTLKARS"
     gene            complement(6703..7092)
                     /locus_tag="PLW13_01560"
     CDS             complement(6703..7092)
                     /locus_tag="PLW13_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY69083.1"
                     /translation="MTRILLVEDNEMNRDMLSRRLARKGFEVLLAIDGEQAVTMTVAE
                     LPDLVLLDMNLPLKDGWSAAREIRADARCRGIPIIALTAHAMSDDRDRALAAGCDDYE
                     TKPLDFARLLDKIAVLLEARGAADAPA"
     gene            complement(7089..8582)
                     /locus_tag="PLW13_01565"
     CDS             complement(7089..8582)
                     /locus_tag="PLW13_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HQY69084.1"
                     /translation="MILRESLASTERLISETISRAEDLRALALLPDLEKCSLAAARAS
                     DCLAADWSGDRQRLLHDLRNHVGAISGYLELLLEEHADIDRRLATNLDALHAQVGELL
                     AATAPEANAALTPPPPAPEGGSGTLLVIDDNELSRELLGRYLRRQGHAVLAAASGAEA
                     IAILQRSAVDLIFLDLVMPEMSGLELLERIKSDERLRAVPVIIVSGIAESDGVIRCIE
                     AGAEDYLSKPFNPTLLQARLNAGLQRKRWHDREVAYRRELERNQRFIRNTFGRYLSDE
                     IVDALLESPQGLDLGGITCRVTILMADIRNFSHVCESRPPQQVVKLLNNYLGAMSAVI
                     MAHNGTVDEFIGDGILAIFGAPVGRPDDARRATRCALAMQAAVHGINARNREQGLPEI
                     AIGIGLNTGVVVAGNIGSEQRSKYGVVGHTVNLTARIESYTGAGEILASQSTVDEAGT
                     GLETGRSFSARPKGMAAEVLIHAITGLRGDDGALPPATAAGTEQSAP"
     gene            complement(8688..9887)
                     /gene="dapC"
                     /locus_tag="PLW13_01570"
     CDS             complement(8688..9887)
                     /gene="dapC"
                     /locus_tag="PLW13_01570"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HQY69085.1"
                     /translation="MNPDLGRLHPYPFERLATLLDGVQPGALAPISLSIGEPKHAAPA
                     FVLEELRRHSAGYSQYPKTQGSAELREAIAAWLTARFGLSAGSLTAAAHVLPVNGSRE
                     ALFAFTQAAIDRSRHAAPLVAMPNPFYQIYEGAALLAGASPLFLPERAERPGIPDFAA
                     VAEADWARCQMLVVCSPGNPTGAVLDLEDWGTIFALADRHDLVVVSDECYSELYPDED
                     APPVGVLEACRHHGRQGFERCIAFHSLSKRSSLPGMRSGFVAGDAALIRSFLLYRTYH
                     GSAMPPPHQAASALAWRDEQHVRENRALYRAKFAAVLAELDGCLEVSAPAGGFYLWPR
                     TPCDEREFARELYRRCNVLVLPGSFLSRPTPHGDPGARRVRMALVAGLEECVEAARRI
                     RGFVATL"
     gene            complement(9884..12577)
                     /gene="glnD"
                     /locus_tag="PLW13_01575"
     CDS             complement(9884..12577)
                     /gene="glnD"
                     /locus_tag="PLW13_01575"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HQY69086.1"
                     /translation="MQEPPHSAEELAERLAGGAPLLPTVREQLARVRADLDARFVAGE
                     NIRTLVRERAQRLDALLACIWSHFGLAGEPGIALLAVGGYGRGELHPHSDIDLLALIT
                     DESVKDRHGAALESFITFLWDTRLAIGHSVRTLDECREVAAGDITIATALMEARTISG
                     DAALLGRLAALTGPEQIWPSAEFFRAKWDEQKARHRKFNNTEYNLEPNIKGCPGGLRD
                     VQTVGWVAKRHFHAASTLELVARGFLTAHEYEALANGQDFLWRIRYGLHMLADRAEDR
                     LLFDHQLSLARLFGYTDDERGLGVEHFMKDYFRCVLKLGVLNEMLTQLFDEAILRACE
                     PVNLYEINNRFRVRNGYIEAAHDNVFRNAPWALIEMFTLMAQHDFIVGVRASTIRLVH
                     GSRRLIGAELRRDPRANRLFLQLLGSPYRVATQLDRMKRYGVLGRFIPEFGRIVGQTQ
                     HDLFHVYSVDSHTIRVVRNMRMFLLPRAREDFPTAAHAIKRLPRPELLYIAGLYHDIA
                     KGRGGDHSNLGAADAIEFCRRLELSTREANLVAWLVQNHLVMSATAQRKDISDPEVII
                     AFARLVSDQVHLDYLYTLTVADINATNPTLWNSWRASLLHSLYVQTKRALRRGLENPI
                     DRGERIAETRAAALGLLGGHGIAPARAEALWAHMGADYFLRETAADIAWHTEAILARA
                     TPEAPVVLTKAAGSRFDPVTQIFIYTRDRDYLFAVITATMEQLGLNVYGARVYSSADR
                     HTLDTFFVLEQDGRPVPPEGPRAGAIRQALLEHIQGEEHYLDVVQRHTPRALKHFSAP
                     TETRLNADPVNGYSILEVMTPDRAGLLARIGRVFLAFGIKVQNARITTLGERVEDVFF
                     ITDANQQPIGDAEMGESLQRAIREALDQGGAAPDTARRALA"
     gene            complement(12581..13363)
                     /gene="map"
                     /locus_tag="PLW13_01580"
     CDS             complement(12581..13363)
                     /gene="map"
                     /locus_tag="PLW13_01580"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQY69087.1"
                     /translation="MPGFIKSPAEIERMRVACRLAAEVLEMIAPFVQPGVSTAELDRI
                     CHEYIVGVQQAIPSPLNYHGFPKSICTSVNQVVCHGIPSDKRILKHGDIINVDITVFK
                     DGYHGDTSAMFLVGKVEAHAERLVRITQECLYRAIEVVRPGARLGDIGHVIQQHAEGN
                     YYSVVREYCGHGIGSQFHEEPQVLHYGRPGTGMELQQGMTFTIEPMINAGKRHTKLSQ
                     RDGWTVETKDGRLSAQWEHTLLVTAGGCEVLTARRGEPWRAG"
     gene            13758..14501
                     /gene="rpsB"
                     /locus_tag="PLW13_01585"
     CDS             13758..14501
                     /gene="rpsB"
                     /locus_tag="PLW13_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQY69088.1"
                     /translation="MTTSVSMKEMLQAGVHFGHQTRYWNPKMKQYIFGARNKVHIINL
                     EHTVPAFNNALEFVQQLSSNKNRLLFVGTKRSASKIVQEEATRAGMPFVSHRWLGGML
                     TNYKTIRASIKRLRELEQQSQDGTFARLTKKEALMRTRLMAKLEQSIGGIKDMGGLPD
                     AIFVVDVDHERIAVTEANKLGIPVIGVCDSNSDPEGIDYLIPGNDDSIRAIRLYVSAV
                     ADACVAGRQEGGVVSRDEFVELGAEAQNG"
     gene            14607..15470
                     /gene="tsf"
                     /locus_tag="PLW13_01590"
     CDS             14607..15470
                     /gene="tsf"
                     /locus_tag="PLW13_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQY69089.1"
                     /translation="MADITAGMVKDLRERTGLGMMECKKALVEANGDIELAIEELRKS
                     SGMKAAKKAGRTAADGVVCVRVAEPGFGLVLEVNSETDFVARDANFLGFVNKVADQAL
                     ANRSDDVAALMGGALEAEREALVQKIGENITVRRARTLEVAAGVVGSYVHGNGKIAVL
                     VGLKGGSAELARDVAMHVAALNPQYATPAEVPAAVVEKEREILIAQAADSGKPADIVA
                     KMIEGRVRKFLSEISLSEQAFVKDPDTTVGKLLKQAGAELAGFVRFEVGEGIEKEVTD
                     FAAEVAAQLKG"
     gene            15628..16347
                     /gene="pyrH"
                     /locus_tag="PLW13_01595"
     CDS             15628..16347
                     /gene="pyrH"
                     /locus_tag="PLW13_01595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQY69090.1"
                     /translation="MHKQKDKKYKRILLKLSGEALGSEGLGIDPRILDRMALEIGQLV
                     GIGVQVGLVIGGGNLFRGAALHEAGMDRVTGDHMGMLATVMNALAMRDALERSNISSA
                     IMSAIPMSGVVDHYDRRKAIRQLSQGDVVIFAAGTGNPFFTTDSAACLRGIEVGAELV
                     LKATKVDGVYTDDPFVNPDAVRFERLSYDEVLARKLGVMDLTAICLCQDHHMPVRVFA
                     MDKPGALLSIVMGGDEGTLIE"
     gene            16366..16923
                     /gene="frr"
                     /locus_tag="PLW13_01600"
     CDS             16366..16923
                     /gene="frr"
                     /locus_tag="PLW13_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQY69091.1"
                     /translation="MINELKKDAESRMRKSVEALGTALNKIRTGRAHPSLLDGVRVSY
                     YGSEMPLSQVANITVEDGRMLVVTPWEKNMVPDVEKAIMKSDLGLNPSTAGTVIRLPM
                     PALTEETRKGYIKQARHEAEAARVAVRNIRKDVNGDLKDLLKDKAISEDEERRALDDV
                     QKLTDRFVAEIDQQLAGKEKDLMEI"
     gene            16986..17714
                     /gene="uppS"
                     /locus_tag="PLW13_01605"
     CDS             16986..17714
                     /gene="uppS"
                     /locus_tag="PLW13_01605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQY69092.1"
                     /translation="MPGHVAVIMDGNGRWARARGLPAVAGHRAGVEVIRKLLRSCREN
                     GVGVLTLFAFSSENWQRPREEVRGLMALFMRYLRGEVRELHSNGIRLRIIGDRTRFAP
                     RLRRLMEESEALTAVNEGFTLVIAADYGGQWDIVQAARALAAEVAAGRLQPGEIDAGA
                     LEAHLSLADLPRPDLLIRTGGDHRISNFLLWQIAYTELYFTECFWPDFGAAQFAAALR
                     DYAARERRFGGRPATVADEGAARC"
     gene            17708..18544
                     /locus_tag="PLW13_01610"
     CDS             17708..18544
                     /locus_tag="PLW13_01610"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQY69093.1"
                     /translation="MLKQRVLTALAFAALVIGVVLYAPVALTAVLFGLLVLGASWEWA
                     ALAGFTTRGARLGYVALVALVLILGARLVGAPAGIEAAAITRVLYPMLGFWLLAALAV
                     LAYPRGAAAWGGRGALAGIGLLVLVPPWLAVLYIRSLPHGEYLVIYAIAVIAFADIGA
                     YFAGRAIGGAKLMPRVSPGKTWAGFAGGMLASLALAACVGAGAGLGGARLGTWVALAG
                     ASALVSVFGDLLESMVKRHGGLKDSGSLLPGHGGLFDRLDSLTAGLPVLAFGLLQAGS
                     FW"
     gene            18544..19728
                     /gene="ispC"
                     /locus_tag="PLW13_01615"
     CDS             18544..19728
                     /gene="ispC"
                     /locus_tag="PLW13_01615"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQY69094.1"
                     /translation="MDERQRVTVLGATGSIGENTLDVIARHAGRYRVFALSCGSRWRE
                     LLAQCRVHAPDYAVVADHAAATELRAAVRAEGLRVEVLAGEAGLVEIAAHPDTDIVMA
                     AIVGAAGLMPTISAARAGKRVLLANKEALVMAGRFFMDAVREGNAALLPIDSEHNAIF
                     QCLPAGFRPGQQSPAVRRLLLTASGGPFRSCPLADLEHVTPAQAVAHPNWVMGRKISV
                     DSATMMNKGLELIEACWLFGMPSSRIEVLIHPQSVVHSLVEFVDGSVLAQLGNPDMRV
                     PIACGLSWPERIESGAGTLDLVAGASLVFEAPDLARFPCLAIAMDAARAGGRAAVVLN
                     AANEIAVQAFLEERIGFTRIAPTIARVLDALGCAEPESLEHVQAIDLEARAVAQRLLK
                     NV"
     gene            19742..21112
                     /gene="rseP"
                     /locus_tag="PLW13_01620"
     CDS             19742..21112
                     /gene="rseP"
                     /locus_tag="PLW13_01620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HQY69095.1"
                     /translation="MDLLYTVLITLVTLGILVAVHEFGHFWVARRCGVKVLRFSIGFG
                     KALWRRTARDGTEYVIAAVPLGGYVKMLDEREGPVAQEELAFSFNRKPVSARMAIVAA
                     GPAANFVLAIVAFYVLYMIGVPGLAPVIGDIAPGSVAEAAGLERGQEIIAVDGEPTPT
                     RQAVAMQLLKRLGESGEIRFSVRYPQTQAGLTYESSARIEDWLMGEDEPDLLGGLGMS
                     YLIPEVPARVAEVVAGSPAERAGMRAGDLIVAVDGEAVASWEAWVGVVRANPGTALAV
                     EVEREQARVMLRVTPAGVQQKDGSRAGQVGVSVAEPQWPPGMLRVDRYGPLAAWRPAL
                     AQTWSLAVFTVDSVKKMFEGLISPKNLSGPITIAKVATDSARSGLESYISFLALLSIS
                     LGVLNLFPIPVLDGGHLVYYLIEMIKGSPVSERAQMLGYQVGLVILVGVMLLAFYNDI
                     NRLAAN"
     gene            21161..23782
                     /gene="bamA"
                     /locus_tag="PLW13_01625"
     CDS             21161..23782
                     /gene="bamA"
                     /locus_tag="PLW13_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQY69096.1"
                     /translation="MKRFLILLPLLLGLARAVFAESFVVTDIRIEGLQRISPGSVFND
                     IPIKVNDRIDQAAIAQTSRALFKSGNFDDVQIGRDGDVLVITLVERPSISEINIDGNK
                     AIKTEDLLEGLKGSGLAVGQVFQRATLEQMRVELQRQYVSQGRYDATIETEVAEQARN
                     RVAVNIDINEGSVARIKHINIVGNTVYGDRDLVDLFELKTTGWFSWLSGNDKYSREKL
                     NGGLESLSSYYLDRGYIRFNIDSTQVAITPDRSAVYITVNVTEGEKYKVTKVDLSGDI
                     VIPESEMRGFLLLRDGQDFSQVQVTNTEEMITQRLGVEGYTFAKVNGIPEIDDENKTV
                     ALKFFVDPGKRTYVRRIEFRGNTKSVDEVLRREMRQMESAPASSAQIEQSRVRVERLG
                     FFKEVKTENVPVPGTDDMIDVQYTVEEQSSGSIGASVGYAQDAGLILGANLQQNNFLG
                     TGKQVGIGLNSSKYQDLYSFNYVNPYYTPDGVSRGFSVFYRSTDLEEINVASYTTDTI
                     GGTMSFGYPISETQRLGLSLGVNRTDISAGIGAVQEISGSPRPIDDAIGYTRQDELND
                     FGYPDLENIRAPVPDEFFKSDIPTGFLDIYGDSFDNFLVTGSWSQFALNRGQLATRGY
                     SQNLSLQVATPGSDLEYYKLTYNGQVFIPLWRQFTLRLRSDLGYGDGWGDTDELPFFE
                     NFFAGGFRSVRGFKANTLGPQSTPAVRYQILNLPVYDAQGNIVDLEDKYVYITCLGPT
                     YDAKGNFLGCTNSGEEKIIHNPVYSGNSENRPFGGDVLVEGSAEVLFPLPFIKDQRSV
                     RTGVFLDFGNVFDTNCSATQVVCSDLDFGELRYSVGFGVTWITGFGPITFSLAKPLND
                     ERTDKTEIFQFSLGQGF"
     gene            23825..24343
                     /locus_tag="PLW13_01630"
     CDS             23825..24343
                     /locus_tag="PLW13_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQY69097.1"
                     /translation="MKRFGRAAVIACLMTLAPGLAFAQGKVAVLNLEAAVFNTEEAKK
                     QIEALRQTPEYLANKKEAEGLKKQYEDLVAQFNKNREVMSAEQEEEQGRKIKQIGTDL
                     EFVAKKLQQSEREVAQRIMREMMPRANTVVMDLLKAEGVGLLLNAQAALFAEPGFSLD
                     AKVTEKLNQTKK"
     gene            24484..>24911
                     /gene="fabZ"
                     /locus_tag="PLW13_01635"
     CDS             24484..>24911
                     /gene="fabZ"
                     /locus_tag="PLW13_01635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQY69098.1"
                     /translation="MLMDVNEVREYLPHRYPFLLVDRVLELDPGKSIVAYKNVSVNEG
                     FFQGHFPDLPVMPGVLIVEAMAQAAGILGFRTMNKKPADGSIYYFVGADDLRFKRPVV
                     PGDRLQLEASILTEKRGIWKFSCRASVDGQTACEATILCA"
     assembly_gap    24912..25011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25108..25722
                     /gene="rnhB"
                     /locus_tag="PLW13_01640"
     CDS             25108..25722
                     /gene="rnhB"
                     /locus_tag="PLW13_01640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQY69099.1"
                     /translation="MTDWRGVLASGVLLAGVDEAGRGPLAGDVIAGAVILDPARPVPG
                     LRDSKKVSRARRPQLAAAVREAAQAWAIGRASVAEIDRLNILQASLLAMRRAVEALAL
                     APQFVLVDGNRCPAWAYPALAVVGGDDLVPAISAASVLAKVERDAEMVALDALYPGYG
                     FAAHKGYPTAEHLAALRRLGASPVHRRSFAPVRAAVAQCALDQE"
     gene            25730..29176
                     /gene="dnaE"
                     /locus_tag="PLW13_01645"
     CDS             25730..29176
                     /gene="dnaE"
                     /locus_tag="PLW13_01645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQY69100.1"
                     /translation="MSAGFVHLRLHSEYSLIDGLVRVDELVERAVALGMPAVALTDHT
                     NLFALIKFYKEAQSAGIKPIVGCDVRVEHEGQTHALTLLVGAQEGYRNLTRLISRGWL
                     EGQQLGRPVLARAWVEEFSGGLIALSGGREGDIGQALLGGRAEQASQLLDYWMALFPE
                     RFYLEVSRTGRAQEEDYLHLAVALAGRHGCPVVATNDVRFLDRSDYEAHEARVCIHEG
                     RTLNDPRRQRQYSPYQYLRSAAEMAELFADLPEALENSVEIARRCNIGIELGKAYLPD
                     YPVPAGMDIATFLGRAAHDGLEERLARVADREAREPEYRARLDFELQTINSMGFAGYF
                     LIVMDFIRWAKNNGVPVGPGRGSGAGSLVAYALGITDLDPLQYQLLFERFLNPERVSM
                     PDFDVDFCMEGRDRVIAYVADTYGREAVSQIITFGTMAAKAVVRDVARVQGKPYGMAD
                     KLSKLIPFEVGMTLEKAMAQEQALRDFVEQNEDVAEIMEMAFKLEGLARNVGKHAGGV
                     VIAPTTLTDFAPLYADDSGGGLVTQFDKDDVEKVGLVKFDFLGLRTLTIIDWALAIIN
                     RRLAVEGRAPIDITTIPLDDASVYALLTRAETTAVFQLESRGMKDLIKRLKPSCFEDI
                     VALVALFRPGPLQSGMVDDFIDRKHGKQAVRYPHPWLEPILENTYGVILYQEQVMQIA
                     QVLAGYTLGGADMLRRAMGKKKPEEMAEQREIFLAGARTNDIDPAVAGSIFDLMEKFA
                     GYGFNKSHSAAYALVSYQTAWLKRHFPAEFMAAVLSSEMHNTDKVVVLIEECRHMKLP
                     LVLPDVNASEFRFTVNARGEVVYGLGAIKGIGEGPVESLVAAREAGGPFRDLFDFCRR
                     TDPRKVNKRALEALIRSGALDGLGASRPVLMAAMGEALKAAEQQARNQDVGLLDMFAD
                     TAAPALAADVYADFAQAPAWSARERLRGEKETLGLYVTGHPIDDHEAEISQFIRTRIV
                     QLRADQEPQRIVGLVVGMRTMKSKRGDDIAFVQLDDRTARIEVTLFADTYRSCREHVV
                     KDNFLVVEGVVSLDDFSGALRMRANEAYTLGEARRRFARELVLQLGAEDFGPDFPEAL
                     GRLLGGGRSREGCAVRVLYSAPDARAEISLGAEWRLSPSDEALGRLRERFGPERVRLR
                     YA"
     gene            29262..30212
                     /locus_tag="PLW13_01650"
     CDS             29262..30212
                     /locus_tag="PLW13_01650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQY69101.1"
                     /translation="MNPNFLDFEQPIAELEAKIEELRLVGNDNEINITEEIEKLRLKS
                     DRLTEKIYSGLTPWQIVKVARHPRRPYTLDYIERIFTDFDELHGDRHFADDNAIVGGM
                     ARLDGIPVMVIGQEKGRSVPEKVLRNFGMPKPEGYRKAMRLMQLAERFALPVITLIDT
                     PGAYPGIDSEERGISEAIAQNLAVMSRLRTPIICVVIGEGSSGGAIGIGVGDHLAMLQ
                     YATYFVISPEGCANIIWKTSEKAPEAAEAMGVTSGVLQELGIVDATIPEPLGGAHRDV
                     DAMATRIREHLATELARLKAVPLEELRETRFQRLMSYGTR"
     gene            30223..31548
                     /gene="tilS"
                     /locus_tag="PLW13_01655"
     CDS             30223..31548
                     /gene="tilS"
                     /locus_tag="PLW13_01655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQY69102.1"
                     /translation="MITPALCLPLLEQFPAARRWVIAYSGGLDSHVLLHLCARLRELR
                     PGVPPVVALHVNHGVQRQADAWTAHCAGVCGALGIPFFARRAELLATDAAQPSEDALR
                     AARYRVFEQFCAGDDLLLLAHQREDQAETVLLRLLRGAGVAGLAGMPRERACGRARLC
                     RPLLETGRAQLRAYAEAEGLRWVEDPSNHCPDYDRNYLRLGVMPLLAARWPGAVMSVA
                     RAAHSLGQAAAICAERADEDLRECAGSDRYGQAFLALATWRALGAARATSALRAWLAR
                     RGAGWPDAQTLATLEREVIGARRDAQPRLVLGAVVVRRYRECLYALPAQSEAGVPVGA
                     AIVPDTPLCLPGAGRIVLGSGRGGGLRPGQTYRIGFGAPGWHCRPAGRPRKTLAQLAQ
                     EHGVPPWLRARLPLLYVDGELAAVADLCVCEGFVAPPGAASLRLDWRPG"
     gene            complement(31631..32215)
                     /locus_tag="PLW13_01660"
     CDS             complement(31631..32215)
                     /locus_tag="PLW13_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69103.1"
                     /translation="MPQLPLVESDKRVLKEDYCNYDLIARFWNDEYRGRAWKNKERIA
                     DYEGTDLEEIIDALRAIVDEIQQEKRRQRGKKKPAAREIADAIIGIEPKLSRTQKMML
                     AIHGKAPGQRLSVKAISRVGDYASAEGAFAEYAEVARRVCDELAYNPGSRKKDHYPGA
                     ALFFEEDITIGSVNADTVLTLRPEIAKALDILKW"
     gene            32465..32561
                     /gene="ffs"
                     /locus_tag="PLW13_01665"
     ncRNA           32465..32561
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLW13_01665"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            32632..34458
                     /gene="dnaX"
                     /locus_tag="PLW13_01670"
     CDS             32632..34458
                     /gene="dnaX"
                     /locus_tag="PLW13_01670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQY69104.1"
                     /translation="MSYQVLARRWRPQTFAQMVGQEHVLRALVNALERNRLHHAYLLT
                     GTRGVGKTTLARLIAKCLNCEQGISASPCGECSACREIAEGRFVDLLEIDAASRTKVE
                     DTRDLLENVQYAPARGRFKVYLIDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPATVLSRCLQLHLKNLSPEQISAHLAHVLREEGIGFEDPALWELAVAAEGSMRDAL
                     SLTDQAIAHGGGSVASATVTEMLGYIERELLRALVEALLQASAENVLAVVADMAAHGA
                     DFERALAELLAVLHRVALAQTVPATLAAMHAERERFAHWAALASPADVQLYYQIGLMC
                     RRDLPYAPDARSGFEMALLRMLAFRPAQPADARSAAAPPSPRATVPRNEPAAQGAVAP
                     PKKPEPAACGPAGVHAAPRPSAPTEPGPAVAPPAALGEAPASAGAPAAEPATPAAGVV
                     SGAAPTQAIRQGDTAPAARLADVNPGNWHRLLAECELGGMAGTVASHCLPEAVEGATL
                     QLLLQQEHEILYQPQCDERIAAALASRFGHAVTVRVRPARVEGETPAERAARLAAERQ
                     QQAVAAIEADANVRMLMENFSGKLHRDSITVPEPADTGREGA"
     gene            34455..34775
                     /locus_tag="PLW13_01675"
     CDS             34455..34775
                     /locus_tag="PLW13_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQY69105.1"
                     /translation="MKGLNDLLKQAQQLQEQVKNAQEDIARQEVQGEAGGGLVRVTMT
                     GRHDVRRVHIEPSLLGEDREVIEDLVAAAVNDAVRRVEALQREKLAGAAGGLSLPEGF
                     KLPF"
     gene            34794..35390
                     /gene="recR"
                     /locus_tag="PLW13_01680"
     CDS             34794..35390
                     /gene="recR"
                     /locus_tag="PLW13_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQY69106.1"
                     /translation="MRFGPLIDQLMEAFQCLPGVGPKSAQRMTLHLLERDRAGGELLA
                     QALERALARVGRCARCRNLSEAQLCPICASDRRDRGVLCVVETPADVLAVEQAGGYAG
                     LYFVLLGRLSPIDGIGPAELGLDLLAQRVRDEGVKEVILATNPTVEGEATAYFITEQL
                     RGAGVAVSRIAHGVPLGGELEYVDGGTLQHAFQGRKPV"
     gene            35387..36538
                     /gene="rnd"
                     /locus_tag="PLW13_01685"
     CDS             35387..36538
                     /gene="rnd"
                     /locus_tag="PLW13_01685"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQY69107.1"
                     /translation="MSSGIVETFNGMPVHFIDAQDALETAAARWLRREVVALDTEFMR
                     VRTFHAQAALFQVCDGEACYLVDPLAVADLAPLARLLDARDVLKVMHSCSEDLEVCER
                     HLGALPEPLVDTQIVAAFCGYPLSVGYQKMLATEFDVHLGKSETRTDWLQRPLSPAQI
                     HYAAEDVAFLPALWRVLAQCLAGKPAGDWAREECAATVARFRARTGADWRALGGAWRL
                     DRRGLAVLSALHAWREETVRTRDLPRSWLVPDAALLRLAELRPQREEQLVAVEDLPEG
                     VRRRYGRAIIEQVATALALEAGELPPAVPPPLDTAQGRRLKALRAHAITRGEALGVAP
                     EMLARRRDFEELLRWCDAGEGAQPPAVLGGWRREVIGEELLALARKGAA"
     gene            36535..36807
                     /locus_tag="PLW13_01690"
     CDS             36535..36807
                     /locus_tag="PLW13_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HQY69108.1"
                     /translation="MKSICAVYRSRRNPDTYLYVDHHEGLSRVPEALQRELGGTERAM
                     TLVLTPQRRLARTSAAEVLRAIREQGYYLQMPPLPEGLRVQGRDAT"
     gene            36804..37247
                     /locus_tag="PLW13_01695"
     CDS             36804..37247
                     /locus_tag="PLW13_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69109.1"
                     /translation="MMNASDYALAWSLYLLACAVVLFTVWGLSARFWSWIKDPLRAVA
                     AVLLLTPGSVDAEGNFLAPAIFIVAFELLTVEDGGIGPLLGVRLLLFVIGAVLAAWVL
                     RALWYWLVASRRTGAVPQAPANPASRTAQLRTVDIRDRSSGRDRS"
     gene            complement(37254..38228)
                     /gene="cysK"
                     /locus_tag="PLW13_01700"
     CDS             complement(37254..38228)
                     /gene="cysK"
                     /locus_tag="PLW13_01700"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQY69110.1"
                     /translation="MNIFPDNSYSIGNTPLVRLNRVAEGKVYAKLESRNPAFSVKCRI
                     GASMIWAAEEDGSLKPGIELVEPTSGNTGIALAFVAAARGYRLTLTMPHTMSLERRQL
                     MTALGANLVLTDGALGMKGAIAKAEEIVARDPARHLMLQQFRNPANPAIHERTTGPEI
                     WRDTEGNIDVFVAGVGTGGTITGVARYIKTTMGKAITAVAVEPASTTMIRDTLAGRTP
                     THAPHKIQGIGAGFVPQNLDLSLIDQVEPVSDDEAMEYAHRLMREEGILAGISCGAAV
                     AAAARVARQPQHLGKTIVVILPDSGERYLRSPLFEGRFSERELVQAMS"
     gene            38447..>38666
                     /locus_tag="PLW13_01705"
     CDS             38447..>38666
                     /locus_tag="PLW13_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY69111.1"
                     /translation="MSFKGTERYVATADLQMAVNAAVTLQRPLLIKGEPGTGKTLLAE
                     EVAIALGKPLIQWHIKSTTKAQQGLYEYD"
BASE COUNT         5872 a        12813 c        13690 g         6191 t
ORIGIN      
        1 cgctcgagct cgatgcgatc gcgttcaaca tcgacgcggc cttctggccc gatccgcggc
       61 gcgaacgcgc acgactcgcc taccggctgg acgtgaacga gtaccgcggc aaccgcagcg
      121 tgcagctgct ggtcgaggaa ctgcaggagc cgactgtcgc gcaggagatg ccatgcagcg
      181 cctgagcgga cgctggcagc tcggcttctg gctggcactg accacggcgg tgctgtgggg
      241 cgcggtgccg ctggcgatga cgccgttgat cgcacatctc gacgcggtca cgatcagctg
      301 gttccgcttc gcttcctcgg gcctgtgcct gctggcgttc ttcgccgcgc gcggacgcct
      361 gcagtggccg cacagcgcag ggcgcaccgc cgcctggctg ttcgcgctcg cggtgctcat
      421 gctcatcggc aactacgtgc tctacgtgca gagcctgcat ttcatcgccg cccccgtggc
      481 gcagaccgtg gtgcaactgg cgccggtgct gctgatgctg ttgagtctct ggctgttcgg
      541 cgagcgcttc acgacatggc agtggggcgg cttcgtgctg ctgctgggcg gcatcacggc
      601 cttctgcgcc gagcggctgc gcgtctcggg cgcgggcatc gacatcttca gcaccggtgt
      661 cgggctgatg gtgctcgcgg ccgcgctgtg ggccgtatac ggcgtggcgc agaagcgcct
      721 gctgcgctac ctgccctcgc agttcgtgct gatgggcatc tatctcgcca gcgcgctgct
      781 gatgctgccg gcggcgagtc ccggcgcgct tgcggcgctc ggcgccaccg agctcgggct
      841 gctcgccttc ctggccgcga acacgctgat cgcctacggc gccttcgccg aggcgctgaa
      901 ccactgggaa tcctcgaagg tgagcgcggt gctggcggtg cagccgctga ccacgctgtt
      961 cggtgccgcg gcgctgggcg cactgtttcc cgcgcacttc cccgcactgc ctctcaccgc
     1021 cggcgccgtg gttgcgtcat tcgcggtggt ggcgggttcg gcgatctgcg cgctgggcgg
     1081 tcgggaggcg cagccaggcg ctgcagactg aggccttcgg ctcggctgga gttgggcgct
     1141 gccgctcggt taaactacgc cgcttttttg aacccccccc aagacatttc ccggagcggc
     1201 aagcatggaa atcaacgcgc tgcagaatca gctgaaggac ctcggcgcgc gcgcgctgag
     1261 cctcaggggg tatctttgac tacgacgtca agaaggaccg cctgaaggaa gtcgagctcg
     1321 agctgagcga acccagtgtc tgggacaacg ccgagcgcgc gcaggagctc gggcgcgagc
     1381 gcgccgcgct ggcggcaatc gtcgacacgc tggagagcct cgagggagcg ctcggcgaca
     1441 gcaccgagct gctggaaatg gccgcggccg agaacgacga gcagacattc gtttccctcg
     1501 aggccgacgt gcagcagctg gcgcgccagg tcgagaccct cgagttccgc cgcatgttca
     1561 gcggcgagca cgacgtcgcc aacgcctacc tcgacatcca ggccggctcc ggcggtaccg
     1621 aggcccagga ctgggccgag atgttgctgc gcatgtacct gcgctggggc gaggccaagg
     1681 gcttcaggac cgagttgatc gagtgcagcg ccggcgaggt cgcgggcatc aagagcgcca
     1741 cgatccactt ccagggcgag tacgccttcg gctggctgcg caccgagacc ggggtgcacc
     1801 gcctggtgcg caaatcgccc ttcgattcgg gcaaccgccg ccacacctcg ttcgcctcgg
     1861 tgttcgtctc ccccgagatc gacgacgcga tcgagatcga gatcaacccc gcggacctgc
     1921 gcgtggatac ctaccgcgcc agcggcgccg gcggccagca cgtgaaccgc accgattcgg
     1981 cggtgcgcat cacgcacgag cccagcggca tcgtggtgca gtgccagtcg cagcgctcgc
     2041 agcaccagaa ccgcgacttc gcgatgaagc agttgcgcgc caagctctac gagatggaga
     2101 tccagaagcg caacgccgtg gcgcaggcgc tggaggacag caagtccgac atcggctggg
     2161 gcagccagat ccgctcctac gtgctcgacc agtcgcggat caaggacctg cgcaccggcg
     2221 tggagaccag caacacccag ggcgtgctcg acggcgacct ggatcgtttc atcgaagcct
     2281 cgctcaagtc cggcctctga cccgaacgga acccacacca tgaccgaaca cgacagcgcg
     2341 caacaggacg agaaccggct gatcgccgag cgccgcgcga agctcgacgc gatccgccgc
     2401 gaacgcaacg ccttccccaa cgatttccgc cgcgagcact acgccgcgga cctgcagcgc
     2461 gactatgccg ggcacagccg cgagcagctc gaggcgctgg gcaagcgcgt cagcgtcgcc
     2521 gggcgcatca tgcgcatgcg cgggccgttc ctcgtgatcg cggacgtggg cgggcagatc
     2581 cagctctacg tgaaccgcga gacgcttccc gccgcgacgc tggccgagat caagacctgg
     2641 gacatcggcg acatcgtcgg cgccagcggc gtgttgcacc gctccggcaa gggcgatctc
     2701 tacgtgaaca tggacagtgc gcggctgctc accaaggcat tgcggccgct gccggacaag
     2761 taccacggcc tcgccgatct cgagacgcgc taccgccagc gctacgtcga cctgatcatg
     2821 aacgaggaca cgcgcaagct ctttcgcacc cgctcggtgg tgctggacgc gctgcgtgac
     2881 tacctcaccg accgcgattt cctcgaggtc gagacgccgg cgatgcaggt gatcccgggc
     2941 ggggcgacgg cgcggccctt cgtgacctac cacaacgcgc tcgatctgga gatgtacctg
     3001 cgcatcgcgc cggaactcta cctgaagcgc ctcgtggtcg gcggcttcga gcgcgtgttc
     3061 gaggtggcgc gcaacttccg caacgagggg ctctcgacgc gccacaatcc cgagttcacg
     3121 atgatcgagt tctacatggc ctatgcggac taccgcgatg ccatggacat caccgagggg
     3181 atgctgcgcc aggccgcgct gcgcggcacc ggtggcacgc tggtcgatta ccagggcgag
     3241 cagtacgacc tgggcgcgcc gttcgcgcgc atgagcgtgt tcgactcgat actgcacttc
     3301 aatccgacgc tcacggccga ggccatcgcc tcgcgcgatc gcgcgcgcca ggtcgccgag
     3361 cacctgggca tcgaggtgaa gcccggctac ggcctgggca agatccagat cgagatcttc
     3421 gagaagaccg tcgagacgcg cctgcgccag ccgaccttca tcaccggcta cccgaccgag
     3481 gtctcgccgc tggcgcggtg caacgacgac gatcccttcg tcaccgaccg cttcgagctg
     3541 ttcatcggcg gtcgcgagct cgccaacggt ttttcggagc tgaacgaccc cgaggaccag
     3601 gcggcgcgtt tccgcgacca ggtcgcgatg aaggagggcg gcgacaacga ggcgatgcac
     3661 ttcgacagcg actacatcac ggcgctcgaa tacggcatgc cgcccaccgc gggcgtgggc
     3721 atcggtatcg atcggctggt gatgctgctc accgattcgc cctcgatccg cgacgtgctg
     3781 ctgttcccgc atatgcggcc gcaggcctga ccctcatgcg ccgggggagc gtacggacgc
     3841 tgcttgcgcc agcgctgctc gtggcgctcg gcggctgcca gggcatgcct accgaggcgc
     3901 cgctgcgcga gccggcaccc gaggcgcagt ccgtgagcgc gccggcgccc gcggcgctgc
     3961 ccgaaaccac gcccgggcag gtgcgcgagg actacgacca ggtgctgcgc caggcatcgg
     4021 cggcctggga gcgcggcgac cacaggagcg cgctgctgtt gtacgaacgc gtgctggaag
     4081 gcgccgcgca gccgcgcgag caggtgcgcg cgctgatcgc catggcgacg ctgcgcctga
     4141 tgccctcgtc gaaggtgacc gatagcgcag ccgccgcggt cgtgatgcgc gagctggacc
     4201 tgcgcctcgc cgcgctcgac ctgcgccacg aatactacgc ccagctcgag ctgctgcgcc
     4261 tgctgcaggc gcgcgagggc gagctgcaga cgctgcgcga atcgaaccgc aagctcgccg
     4321 cgcagctcgc cgcgaaagac gagctggtgc gtcagctgcg cgccttgagc gtcgacggcg
     4381 gctgagaagc cgccgcgatc cggccggtct gctcgagcag cgattccacc gagccggcgt
     4441 cgtgcaacac cagccggtcg aggtgcgagg ccagccgcgc gtgcgcatcg gcgtgggtgt
     4501 cggcgccggc gagcacgaat accggcaccc ggctcagcaa cgggctgcgg ccgagttcct
     4561 cgctgagcgc gaacacatcc tcgctgggca ggcccagcga cagcaggatc aggtccggca
     4621 ggctctggcg cgcgatcgcc agcgcctcgc ccgcctgttc cgtgctgctc acgtaccaac
     4681 cctgtccctg cagtcccgcg atcagttcct cgcgcgcttg cgattcgctc tcgatcagca
     4741 gcacgtggcc gctgcggtcg cgcggggcga gacgctcgag cgtctcggcc agacggcgcc
     4801 ggttcacggg cttgtcgagg aaatccgcgg cgcccttgct catcgcctcg tcgcgcgcat
     4861 cgagcatcga ttgcagcacc accgggatgt cacgcgtctc ctcgttgtct ttcagcaccg
     4921 agagcacggc ccagccgtcc atgcccggca tcaccaggtc gagcacgatc aggtccggcc
     4981 gctgctcgcg cgccagcctg aggccctcgt cgccgctctc ggcctcgagc acgcggcagc
     5041 cctcgccgcg cagcacgcgc gcgctgatgg tgcgcgcgtc ggcgtcgtcg tcgatcacca
     5101 gcaccgtctg cgcgcccgat ggcgccgccg tcggcaaggc cgcggtttcc gcgccaggcg
     5161 ccgccgtggc aaagaacagc tccggcacgg tcgtggcatc caccggcagc tccacggtga
     5221 acacgctgcc ttggtccggt tcgctggcca caccgatcgt gccgccgagc agctggcaga
     5281 actcgcggca cagcgacagc cccagcccgg tgccgccgta gcgcttggtg gtcgaggaat
     5341 cggcctgcac gaaggcctcg aacacgctgt cgagctgtgc gggcgtcatg ccgatgccgg
     5401 tgtcgcgcac gcgaaagcgc agccagtccg cgccttcgcg cgtgaagggc tcggtgtcca
     5461 ccgtgatctc gccgtccgtg gtgaacttgc aggcgttgct gatgaggttg gcgaagatct
     5521 ggcgaaagcg cgtctggtcg ctgaccagcg ccgcctcgtg cggtgtcgcg gcgatgcgga
     5581 tcgtgttgcc gttgtgcgcg gccagcgtgt gcatggtggt gccgagctcg cgcagcacct
     5641 cgatcgcgtc gaagggctcg cgatagagcg tcatcttgcc ggtctcgatc ttggcgagat
     5701 cgagcacgtt ggtgatcagg gagagcaggt gcctgcccgc ggtgaggatg cgctgcatgt
     5761 cgccgctgag ttcggcggcg ggcaaatcct gctcttcggc ggccatctcg accatcatct
     5821 cgctgtagcc gatgatcgcg ttgagcggcg tgcgcagctc gtggctcatg ttggcgagga
     5881 agccgctctt ggcggcgttg gcgcgatcgg cctgctcctt ggcttcctgc acctccagga
     5941 ccgcggcgcg cagcttctcc gacatgtcgt tgaaggccgc ggcgagctgt ccgaactcgt
     6001 cgtgcgaggg caccgtgatg cggtagtcga ggtcgccgcc gccgatcctg accgtgccct
     6061 cgcgcaggcg catcagcgac tcgttggtgt ggcggatcag caggaagccg agcaggctgg
     6121 tcacgaagat cgagctgagg aagatgatca cggtcacgcg cccgatggtg cgcccggtcg
     6181 actcgacgtg ctcgctctgc gcgcgcgaga ccgcgatcac ggctgcctcg aaatccccca
     6241 gcgcacgctc gaagccggcg taggcgactt tcagctccgc cgcggacagg ccgtgcgtgc
     6301 cttggcggcg cagttgcgac agcacgttct cccagtcgcc gaacagcgtt tgcgcgtgcg
     6361 cctgcagtgc ggcgtgcgcg gcggcggaat cctcgttggt gttgcgttcc atgcgggcga
     6421 tcagctggcg cagcccggcg attgcctcgc gcgtgcgctc ggcctcgggg ccggagatgc
     6481 cctggccggt cgattcctgc agtgtcacca gcaccagcag ctgcttgtgc aggtcgtcca
     6541 tgtcctggcg cacggtgctg gcgttgatct ggccctgcac cgcgtcgctg accgcatcga
     6601 gcgaatcgcg gatggtgcgg ttgccgatcg aatagctgac cacgttgacc gcgaacagca
     6661 gcagcacgct gaagatcgcg atcgtcagcc gttgcagcag gctcatgccg gggcgtccgc
     6721 ggcgccgcgt gcctcgagca gcaccgcgat cttgtcgagc agccgcgcga agtcgagcgg
     6781 cttggtctcg tagtcgtcgc agcccgccgc cagcgcacgg tcgcggtcgt cggacatcgc
     6841 gtgcgcggtg agcgcgatga tcgggatgcc ccggcagcgc gcgtcggcgc ggatctcgcg
     6901 cgccgcgctc cagccgtcct tcagcggcag gttcatgtcc agcagcacga ggtcgggcaa
     6961 ctcggccacg gtcatcgtga cggcctgctc gccgtcgatc gccagcagca cctcgaagcc
     7021 cttgcgcgcc aggcgccgcg agagcatgtc gcggttcatc tcgttgtcct cgaccaggag
     7081 gatgcgtgtc atggcgcgct ttgctccgtg ccggcggcgg tggcgggggg gagcgcgccg
     7141 tcgtcgccgc gcagcccggt gatggcatgg atcaggacct cggcggccat gcccttgggt
     7201 cgcgcgctga agctgcgccc ggtctcgagg ccggtaccgg cctcgtcgac cgtgctctgc
     7261 gaggcaagga tctcgccggc gccggtgtag gactcgatgc gcgcggtgag gttgaccgtg
     7321 tgcccgacca cgccgtactt gctgcgctgc tcggagccga tgttgccggc caccaccacg
     7381 ccggtgttca ggccgatgcc gatggcgatc tcgggcagcc cctgttcgcg gttgcgcgcg
     7441 ttgatgccgt gcaccgcggc ctgcatcgcg agcgcgcagc gcgtggcgcg ccgggcgtcg
     7501 tcggggcggc ccaccggcgc gccgaatatc gcgagaatgc cgtcgccgat gaattcgtcc
     7561 acggtgccgt tgtgcgccat gatcacggcg gacatcgcgc cgaggtaatt gttcagcagt
     7621 ttgaccacct gctggggcgg gcggctctca cagacatggc tgaagttgcg gatgtcggcc
     7681 atcaggatcg tgacccggca ggtgatgccg cccaggtcca gtccctgcgg cgactcgagc
     7741 agcgcatcga cgatctcgtc cgagaggtag cgcccgaagg tgttgcggat gaagcgctgg
     7801 ttgcgctcga gctcgcgccg gtacgccacc tcgcggtcgt gccagcgctt gcgctgcagg
     7861 ccggcgttga ggcgcgcctg cagcagcgtg ggattgaagg gtttggacag gtagtcctcg
     7921 gcgccggcct cgatgcagcg gatcacgccg tcgctctccg cgatgcccga gacgatgatc
     7981 acggggaccg cgcgcaggcg ctcgtcggac ttgatgcgct cgagcagttc cagcccgctc
     8041 atctccggca tcacgaggtc gaggaagatc aggtccacgg cgctgcgctg caggatcgcg
     8101 attgcctcgg cgccggaggc ggcggcgagc acggcgtgcc cctggcggcg caggtagcgc
     8161 cccagcagtt cgcggctgag ctcgttgtcg tcgatcacca gcagggtgcc gctgccgccc
     8221 tcgggcgcgg gcggcggcgg ggtgagcgcg gcattggcct cgggcgcggt cgcggcgagc
     8281 agttcgccga cctgcgcgtg cagggcgtcg aggttcgtcg cgagccggcg gtcgatgtcc
     8341 gcgtgctcct cgagcagcag ttcgaggtag ccgctgatcg cgccgacgtg gttgcgcaga
     8401 tcgtgcagca ggcgctggcg gtcgcccgac cagtctgcgg ccaggcagtc cgaggcgcgc
     8461 gccgcggcga gcgagcactt ttccaggtcg ggcagcagcg cgagcgcgcg caggtcctcg
     8521 gcgcgcgaga tcgtctccga tatcagccgc tcggtgctgg cgagggattc tcgcaggatc
     8581 acggaatctg gtgcgtgcgc ggcggacacc cgttgggcct gtgcatgtgc ttcggggtat
     8641 ttatacccgc attgttcccg ggctggaaac cccgggagcc gccgcgttca gagcgtcgcc
     8701 acgaagccgc ggatgcgccg cgcggcctcg acgcattcct cgagtccggc gaccagcgcc
     8761 atgcgcacgc gtcgcgcgcc gggatcgccg tgcggcgtgg ggcggctgag gaagctgccg
     8821 ggcagcacca gcacgttgca gcgccggtag agctcgcgcg cgaactcgcg ttcgtcgcag
     8881 ggcgtgcgcg gccacaggta gaagccgccg gcaggcgcgc tcacctcgag acagccgtcg
     8941 agctcggcca gtaccgcggc aaacttcgcg cggtacaggg cgcggttttc gcgcacgtgc
     9001 tgctcgtcgc gccaggccag cgcgctcgcc gcctggtgcg gcggcggcat cgccgagccg
     9061 tggtaggtgc gatagagcag gaaggagcgg atcagcgcgg cgtcgccggc cacgaagccc
     9121 gagcgcatgc cgggcaggct ggagcgcttc gacaggctgt ggaacgcgat gcagcgctcg
     9181 aagccctgcc gcccgtggtg gcggcaggcc tcgagcacgc ccaccggcgg cgcgtcctcg
     9241 tcggggtaga gctccgaata gcattcgtcg gacaccacca cgaggtcgtg gcgatccgcc
     9301 agcgcgaaga tcgtgcccca gtcctcgaga tccagcaccg cgccggtcgg gttgccgggc
     9361 gagcagacca ccagcatctg gcagcgcgcc cagtcggcct cggcgacggc cgcgaagtcg
     9421 gggatgccgg gccgctcggc acgctcgggc aggaacagcg gcgaggcgcc ggcgagcaac
     9481 gccgcgccct cgtagatctg gtagaacggg ttcggcatgg cgaccagcgg cgcggcgtgg
     9541 cggctgcggt cgatggcggc ctgcgtgaag gcgaacagcg cctcgcggct gccgttgacg
     9601 ggcaacacgt gcgccgccgc tgtcaggctg cccgcggaca gaccaaagcg cgccgtgagc
     9661 caggccgcga tcgcctcgcg cagttccgcg ctgccctggg tcttggggta ctgcgagtag
     9721 ccggcgctgt ggcggcgcaa ctcctccagc acgaaggcgg gcgcggcgtg cttgggctcg
     9781 ccgatcgaca gcgagatcgg cgccagcgcc cccggctgca cgccgtccag cagcgtggcg
     9841 aggcgctcga aggggtaggg atgcaggcgc ccgaggtccg ggttcatgcc agtgcgcgcc
     9901 gcgcggtgtc gggcgccgcg ccgccctggt cgagcgcctc gcggatcgcg cgctgcagtg
     9961 attcgcccat ctccgcgtcc cctatcggtt gctggttggc atcggtgatg aagaaaacgt
    10021 cctcgacgcg ctcgcccagc gtggtgatgc gcgcgttctg caccttgatg ccgaaggcga
    10081 ggaacacgcg cccgatgcgc gcgagcagtc cggcgcgatc cggtgtcatc acctcgagta
    10141 tcgagtagcc gttgaccgga tcggcgttga ggcgcgtctc cgtgggcgcg ctgaagtgct
    10201 tgagcgcccg cggcgtgtgg cgctggacga cgtcgaggta atgctcctcg ccctggatgt
    10261 gctcgagcag cgcctgccgg atcgccccgg cgcgcggccc ctcgggcggc accgggcgcc
    10321 cgtcctgctc gagcacgaag aaggtgtcca gcgtgtggcg gtcggccgag gaatagaccc
    10381 gcgcgccgta cacgttgagt cccagctgtt ccatcgtcgc cgtgatcacc gcgaacaggt
    10441 agtcgcggtc gcgcgtgtag atgaagatct gggtcaccgg gtcgaagcgg ctgccggctg
    10501 ccttggtcag caccaccggc gcctccggcg tggcgcgcgc caggatcgcc tcggtgtgcc
    10561 aggcgatgtc ggccgcggtc tcgcgcagga agtagtccgc ccccatgtgc gcccacagcg
    10621 cctcggctcg cgcgggcgcg atcccgtggc ccccgagcag cccgagcgcc gcggcccggg
    10681 tctcggctat gcgctcgccg cggtcgatgg ggttctcgag gccgcgccgc agtgcgcgct
    10741 tggtctgcac gtagagcgag tgcagcaggc tcgcccgcca gctgttccac agcgtggggt
    10801 tggtcgcgtt gatgtcggcc accgtgagcg tgtacaggta gtcgaggtga acctggtcgg
    10861 acaccaggcg cgcgaaggcg atgatcacct ccgggtccga gatgtccttg cgctgcgccg
    10921 tggccgacat cacgaggtgg ttctgcacca gccacgccac gaggttggcc tcgcgcgtgc
    10981 tcaactcgag gcggcgacag aattcgattg cgtccgcggc gcccaggttc gagtggtcgc
    11041 cgccgcggcc cttggcgatg tcgtggtaca gccccgcgat atagagcagt tccgggcgcg
    11101 gcaggcgctt gatcgcgtgt gcggcggtcg ggaaatcctc gcgcgcgcgc ggcagcagga
    11161 acatgcgcat gttgcgcacg acgcggatgg tgtgcgaatc gaccgagtag acgtggaaca
    11221 ggtcgtgctg ggtctggccg acgatgcgcc cgaattcggg gatgaaacgc ccgagcacgc
    11281 cgtagcgctt catgcggtcg agctgggtcg cgacgcggta gggcgagccg agcagttgca
    11341 ggaacaggcg gttggcgcgc gggtcgcgcc gcagctcggc gccgatcagc cggcgcgagc
    11401 cgtgcacgag acggatcgtc gaggcgcgca cgccgacgat gaagtcgtgc tgcgccatca
    11461 gcgtgaacat ctcgatcagc gcccacggcg cgttgcggaa cacgttgtcg tgggccgcct
    11521 cgatgtagcc gttgcgcacg cggaagcgat tgttgatctc gtagagattg accggctcgc
    11581 aggcgcgcag gatcgcctcg tcgaagagtt gcgtgagcat ctcgttcagc acgccgagct
    11641 tcagcacgca gcggaagtag tctttcatga agtgctcgac gccgaggccg cgctcgtcgt
    11701 cggtgtagcc gaacagcctg gccaggctca gctggtgatc gaacagcagc cggtcctcgg
    11761 cgcggtcggc cagcatgtgc agaccgtagc ggatgcgcca caggaagtcc tgtccgttgg
    11821 cgagcgcctc gtactcgtgc gcggtcagga agccgcgcgc gaccagctcc agcgtgctgg
    11881 cggcatggaa gtggcgtttc gccacccagc ccacggtctg cacatcgcgc aacccgcccg
    11941 ggcagccctt gatgttcggt tcgaggttgt actcggtgtt gttgaacttg cggtggcgcg
    12001 ccttctgctc gtcccacttg gcgcggaaga attccgcgct cggccagatc tgctccgggc
    12061 cggtcagcgc ggcgagccgc ccgagcagcg cggcatcgcc gctgatcgtg cgcgcctcca
    12121 tcagcgcggt tgcgatcgtg atgtcgccgg cggcgacctc gcggcattca tccagcgtgc
    12181 gcacgctgtg gccgatcgcc agacgggtgt cccacaggaa cgtgatgaac gattccagtg
    12241 ccgcgccatg gcggtccttc acggattcgt cggtgatcag cgccagcagg tcgatgtcgg
    12301 agtgggggtg caactcgccg cgaccgtagc cgccgacggc gagcagcgcg atgcccggtt
    12361 cgccggccag cccgaagtgg ctccagatgc aggccagcag agcatccagg cgttgcgcgc
    12421 gctcgcgcac cagggtgcgg atgttttcgc cggccacgaa gcgcgcgtcg aggtcggcgc
    12481 gcacgcgcgc cagctgttcg cgcaccgtcg gcagcagcgg cgccccgccg gcaagacgct
    12541 cggccagttc ctcggcgctg tgcggaggct cctgcatggt tcagccggcg cgccagggct
    12601 cgccgcggcg tgccgtgagc acctcgcagc ccccggcggt caccagcagc gtgtgctccc
    12661 actgcgccga cagccgcccg tccttggtct ccacggtcca gccgtcgcgc tgcgacagct
    12721 tggtgtgtcg cttgccggcg ttgatcatcg gctcgatcgt gaaggtcatg ccctgctgga
    12781 gttccatgcc ggtgccgggg cggccgtaat gcagcacctg gggctcctcg tggaactggc
    12841 tgccgatgcc gtggccgcag tactcgcgca ccacggagta gtaattgccc tcggcgtgct
    12901 gctggatcac gtgcccgatg tcgcccaggc gtgcgccggg gcgcaccacc tcgatggcgc
    12961 ggtagaggca ttcctgcgtg atgcgcacca ggcgctcggc atgggcctcg accttgccga
    13021 ccaggaacat cgcgctggtg tcgccgtggt agccgtcctt gaagacggtg atgtcgacgt
    13081 tgatgatgtc gccatgcttg aggatgcgct tgtcgctggg gatcccgtgg cagaccacct
    13141 ggttcaccga ggtgcagatc gacttcggga agccgtgata gttcagcggc gacggtatgg
    13201 cctgctgcac cccgacgatg tactcgtgac agatgcggtc gagctcggcc gtgctgacgc
    13261 ccggctgcac gaacggcgcg atcatctcga gcacctcggc ggcgaggcgg caggccacgc
    13321 gcatgcgctc gatctcggcg gggctcttga tgaatcccgg catcgggttc ggctggcttc
    13381 tcatgctgct gtcgggttcc ggggtagagt gcattggtgt cgggccgcgg gggacgggca
    13441 gattgtaaac cattcgcaat ccccggcgcg ccccgcgacg gcgggcaaaa aactgtcatt
    13501 agtcgccggc ccgggaggcc cggcttcagt tccgggggtg gctgtggtat aaagccgccc
    13561 gcttcgcaag ggactcgttc cccgcgggcg ggacgctgcc gcggcagccg ttgccaaccg
    13621 gcacggcgcc ggacgagcgg gtccgaacgt cacacatgca ccggcacatg gctggggtgc
    13681 ccgtcgcaac gccaggcgta gtgcgggtca gaccatgggg tgcatggagg ctcaaccctg
    13741 atatcaaagg tgcaatcatg actacgtccg tttcgatgaa ggaaatgctg caggccggcg
    13801 tccacttcgg ccaccagacc cgttactgga acccgaagat gaagcaatac atcttcggcg
    13861 cccgcaacaa ggtccacatc atcaacctgg agcacacggt tcccgcgttc aacaacgcgc
    13921 tggaattcgt gcagcagctc tcgtccaaca agaaccgcct gctgttcgtg ggcaccaagc
    13981 gctcggcgag caagatcgtc caggaagagg cgacgcgcgc cggcatgccc ttcgtgagcc
    14041 atcgctggct tggcggcatg ctgaccaact acaagacgat ccgtgcctcg atcaagcgcc
    14101 tgcgcgagct cgagcagcag agccaggacg gcaccttcgc gcgcctcacc aagaaggaag
    14161 cgctgatgcg cacgcgcctg atggcgaagc tcgagcagag catcggcggc atcaaggaca
    14221 tgggcggcct gcccgatgcg atcttcgttg tcgacgtcga tcacgagcgc atcgcggtga
    14281 ccgaagccaa caagctcggc atccccgtca tcggcgtctg cgacagcaac agcgacccgg
    14341 aaggcatcga ctacctgatc cccggcaacg acgactccat ccgtgcgatc aggctctacg
    14401 tgtccgcggt ggcggacgcg tgcgtggcgg gtcgccagga aggcggcgtg gtgtccaggg
    14461 acgagttcgt cgagctgggt gccgaagcgc agaacggctg accggaacct gccggctggc
    14521 gtctcgcggc cggcttcaca tggattgcgg cggcccggtg gccgccgctt tcgcttgcat
    14581 aacctgtttt ttcggaggac atcgggatgg ctgatatcac ggctggtatg gtgaaggatc
    14641 tgcgcgagcg caccggtctg ggcatgatgg agtgcaagaa ggcgctggtc gaggcgaatg
    14701 gcgacatcga gctcgccatc gaggagttgc gcaagtcgag cggcatgaaa gcggccaaga
    14761 aagccggtcg caccgcggcc gacggcgtgg tctgcgtgcg tgtggccgag ccgggcttcg
    14821 ggctggtgct cgaagtgaac agcgaaacgg atttcgtggc ccgtgacgcc aactttctcg
    14881 gcttcgtcaa caaggtggcc gatcaggcgc tcgcgaaccg cagcgatgac gtggcggccc
    14941 tgatgggcgg cgcgctggaa gccgagcgcg aggcgctggt gcagaagatc ggcgagaaca
    15001 taacggtgcg ccgtgcgcgc acgctcgaag tcgccgccgg cgtggtcggc agctacgtgc
    15061 acggcaacgg caagatcgcc gtgctggtgg gcctgaaggg cggttccgcc gaactggccc
    15121 gcgatgtggc catgcacgtc gccgctctca acccgcagta cgccacgccg gccgaggtgc
    15181 ccgcggcggt cgtcgagaag gagcgcgaga tcctgatcgc gcaggccgcc gacagcggca
    15241 agccggccga catcgtcgcc aagatgatcg agggccgcgt gcgcaagttc ctgtccgaga
    15301 tcagcctgtc ggaacaggcc ttcgtgaagg atcccgacac caccgtcggc aagctgctga
    15361 agcaggcggg cgccgagctc gcgggcttcg tgcgcttcga ggtgggcgag ggcatcgaga
    15421 aggaagtgac ggacttcgcg gccgaggttg cggcgcagct caagggctga acacgctcgc
    15481 aggccacggc agccaccagg gcgcccccgc aggggggcgc ctttttttta tccgtgccgc
    15541 gccggcgcgc tgccggaaaa tgttacgcag acgccgcgcg ccgttagctg tagacttgcg
    15601 cgcggtcaat ggctgtcggg cgtgtcgatg cacaagcaga aagacaagaa atacaagcgt
    15661 atcctgctca agctgagcgg cgaggcactg ggcagcgaag gtctcggcat cgacccgcgc
    15721 atactcgacc gcatggcgct cgagatcggc cagctggtcg gcatcggcgt gcaggtcggg
    15781 ctggtgatag gcgggggcaa cctgttccgt ggtgccgcgt tgcacgaggc gggcatggac
    15841 cgcgtcaccg gcgaccacat gggcatgctg gccaccgtga tgaacgcgct ggcgatgcgc
    15901 gatgcgctcg agcgctccaa tatctcctcg gccatcatgt cggcgatccc gatgagcggc
    15961 gtcgtcgacc actacgaccg gcgcaaggcg atccgccagc tctcgcaggg cgacgtggtc
    16021 attttcgccg ccggcaccgg caacccgttt ttcaccaccg attcggcagc ctgcctgcgc
    16081 ggtatcgagg tgggagcgga actggtgctg aaggccacca aggtcgacgg tgtctacacc
    16141 gacgatccgt tcgtcaatcc ggatgcggtg cgcttcgagc gcctgagcta cgacgaagtg
    16201 ctcgcacgca agctcggcgt gatggacctc acggcgatct gcctgtgcca ggaccaccac
    16261 atgccggtgc gcgtgttcgc gatggacaag cccggcgcgt tgctcagcat cgtcatgggc
    16321 ggtgacgaag gcacactgat cgaataagca gcagaggaat ccagcgtgat caacgaactc
    16381 aagaaggatg cggagtccag gatgcgcaag agcgtcgagg cgctcggcac cgcgttgaac
    16441 aagattcgca cgggccgcgc gcaccccagc ctgctcgacg gcgtgcgcgt gtcctactac
    16501 ggcagcgaga tgccgctcag ccaggtggcc aacatcacgg tggaggacgg gcgcatgctg
    16561 gtcgtcacgc cgtgggaaaa gaacatggtg cccgatgtgg aaaaggccat catgaagtcc
    16621 gacctgggcc tgaacccctc gaccgcgggc accgtgatcc gcctgccgat gcccgcgctc
    16681 accgaggaga cgcgcaaggg ctacatcaag caggcgcggc acgaagccga ggccgcgcgc
    16741 gtcgcggtgc gcaacatccg caaggacgtc aatggcgacc tcaaggacct gctgaaggac
    16801 aaggcgatct ccgaggacga ggagcgccgc gcgctcgacg acgtgcagaa gctcacggac
    16861 cgtttcgtcg ccgagatcga ccagcagctc gccggcaagg aaaaggacct gatggaaatc
    16921 tgagcgccgc gcgacagccg gacgctaccg tgctgcctcc cgccacgtct gccagtgacg
    16981 cccggatacc gggccacgtg gccgtcatca tggacggcaa cgggcgctgg gccagggcgc
    17041 gtggcctgcc ggcggtggcc ggtcaccgcg ccggcgtcga ggtcatccgc aagctgctgc
    17101 gctcctgccg ggaaaacggc gtcggggttc tgacgctgtt cgccttcagc agcgagaact
    17161 ggcagcgccc gcgcgaggag gtgcgcggcc tgatggcgct gttcatgcgc tacctgcgcg
    17221 gcgaggtgcg cgagctgcac agcaacggca tccgcctgcg catcatcggc gaccgcacgc
    17281 gcttcgcgcc gcggctgcgc cggttgatgg aggaatccga agccctgacc gcggtcaacg
    17341 agggcttcac cctggtgatc gcagccgatt acggcggcca gtgggacatc gtgcaggctg
    17401 cccgcgcgct ggccgccgag gtcgccgcgg ggcgcctgca gcccggcgag atcgacgccg
    17461 gcgcgctgga ggcgcatctg agcctcgccg acctgccgcg cccggacctg ctgatccgca
    17521 ccggcggcga ccatcgcatc agcaatttcc tgctgtggca gatcgcctac acggagctgt
    17581 atttcaccga gtgcttctgg ccggatttcg gcgctgcgca attcgccgcc gcgctgcgcg
    17641 actacgcggc gcgcgagcgc cgcttcgggg ggcgcccggc gaccgtggcg gacgagggag
    17701 cggcgcgatg ctgaagcagc gcgtcctcac cgcactggcg tttgccgccc tggtcatcgg
    17761 cgtggtgctg tacgccccgg ttgcgctcac cgcggtgctg ttcggcctgc tggtgctcgg
    17821 tgcctcgtgg gaatgggccg cgctcgccgg gttcacgacg cgcggcgcgc gcctgggcta
    17881 cgtcgccctg gtcgccctgg tcctgatcct gggtgcgcgc ctggtggggg cgcccgccgg
    17941 gatcgaggcc gcggcgatca cgcgcgtgct ctaccccatg ctgggcttct ggctgctagc
    18001 agcgctcgcg gtgctagcct atccgcgtgg cgccgccgcc tggggaggcc gcggcgcgct
    18061 tgcgggtatc gggctgctgg tgctggtgcc gccgtggctc gcggtgctgt acatccgctc
    18121 gctgccccac ggcgaatacc tggtcatcta cgcgatcgcg gtgatcgctt tcgcggacat
    18181 cggcgcctac ttcgccgggc gcgcgatcgg cggcgcgaaa ctgatgccgc gggtcagccc
    18241 cggcaagacc tgggccggat tcgccggcgg catgctggcg agcctggcgc tcgccgcctg
    18301 cgtcggggct ggcgcggggc tgggcggggc gcggctgggc acctgggtgg cgctggcggg
    18361 cgcgagcgcc ctggtttcgg tgttcggcga tctgctcgaa agcatggtga agcgccatgg
    18421 cggattgaag gacagcggct cgctgctgcc cggacacggc ggcttgttcg accggctgga
    18481 cagcctcacc gcgggactgc cggtgctggc cttcggactg ctgcaggcgg ggagtttctg
    18541 gtgatggacg agcggcagcg ggtcacggtc ctgggggcaa cgggctccat cggcgagaac
    18601 acgctcgacg tgatcgcgcg ccacgccggg cgctaccggg tgttcgcgct cagctgcggc
    18661 tcgcgctggc gcgagctgct cgcgcagtgc cgcgtgcacg cgcccgatta cgcggtggtg
    18721 gcggaccacg ccgcggccac cgagttgcgc gcggcggtgc gtgccgaagg gctgcgggtc
    18781 gaggtgctgg cgggcgaggc gggcctggtc gagatcgccg cgcaccccga taccgacatc
    18841 gtcatggcgg ccattgtcgg tgcggccggg ctgatgccca cgatcagcgc cgcgcgcgcc
    18901 ggcaagcgcg tgctgctggc gaacaaggaa gcgctggtga tggcggggcg cttcttcatg
    18961 gatgccgtgc gcgagggcaa tgccgcgctg ctgcccatcg acagcgaaca caatgcgatc
    19021 ttccagtgcc tgcccgctgg ctttcgtccc gggcagcagt cgccggcggt gcgccggctg
    19081 ctgctcaccg cctccggcgg gccgtttcgc agctgcccgc tggccgatct cgagcacgtg
    19141 acgccggcgc aggcggtggc gcacccgaac tgggtgatgg ggcgcaagat ctcggtcgac
    19201 tcggccacca tgatgaacaa ggggctggag ctgatcgagg cctgctggct gttcggcatg
    19261 ccgagttcgc gtatcgaggt gctgatccac ccgcagagcg tggtgcattc gctggtcgag
    19321 ttcgtcgacg gctcggtgct ggcgcagctg ggcaatcccg acatgcgggt gccgatcgcc
    19381 tgcggcctgt catggccgga gcgcatcgag tcgggcgcgg gcacgctcga cctggttgcc
    19441 ggcgcgtcac tggtcttcga ggcgccggat ctcgcgcgtt ttccctgcct ggcgatcgcg
    19501 atggacgccg cgcgcgcggg ggggcgcgcg gcggtggtgc tcaatgccgc caacgagatc
    19561 gcggtgcagg cctttctcga ggagcgcatc ggctttaccc gcatcgcgcc gacaattgcc
    19621 cgggtgctcg atgcgctcgg ctgcgcagaa ccggaatcgc tggaacatgt ccaagcaata
    19681 gacctcgagg cgcgtgccgt cgcgcagcgc ctgctgaaga acgtctgagg agggaggaga
    19741 catggatctg ctgtatacgg tgctgatcac cctggtgacc ttgggaatcc tggtggcggt
    19801 gcacgagttc ggccatttct gggtcgcgcg gcgttgcggt gtgaaggtgc ttcgcttctc
    19861 gatcggcttc ggcaaggcgc tgtggcggcg caccgcccgc gacggcaccg agtacgtgat
    19921 cgccgcggtg ccgctgggcg gctacgtgaa gatgctggac gaacgcgagg gtccggtcgc
    19981 gcaggaggag ctggcgttca gcttcaatcg caagccggtg tcggcgcgca tggcgatcgt
    20041 cgcggccggt ccggcggcga atttcgtgct cgcgatcgtc gcgttctacg tgctctacat
    20101 gatcggcgtt cccggcctgg ccccggtgat cggcgatatc gcgccgggtt cggtcgccga
    20161 agccgcgggg ctcgagcgcg ggcaggagat catcgcggtc gatggcgagc ccaccccgac
    20221 gcgccaggcg gtcgcgatgc agctgctgaa gcggctcggc gagagcggcg agatccgctt
    20281 ctcggtgcgc tatccgcaga cgcaagccgg tctcacctac gagtcctcgg cccggatcga
    20341 ggactggctc atgggcgagg acgagcccga cctgctcggc ggcctgggga tgtcctacct
    20401 gatacccgag gtgccggcgc gcgtcgccga ggtcgttgcc ggcagtcccg cggaacgcgc
    20461 cggcatgcgt gcgggcgacc tgatcgtcgc ggtggacggc gaggccgtgg cgtcctggga
    20521 ggcgtgggtc ggcgtggtgc gcgcgaatcc cggcaccgcg ctcgcggtag aggtcgagcg
    20581 cgagcaggca cgcgtgatgc tgcgcgtcac gcccgccggc gtgcagcaga aggacggcag
    20641 ccgtgcgggg caggtcggcg tcagcgtcgc cgagccgcaa tggccgcccg ggatgctgcg
    20701 cgtggaccgt tacgggccgc tcgcggcgtg gcgcccggcg ctggcgcaga cgtggtcgct
    20761 ggccgtcttc accgtcgatt ccgtgaagaa gatgttcgag ggcctgattt cgccgaaaaa
    20821 cttgagcggc cccatcacga ttgctaaagt ggcgaccgat tcggcgcgct cgggactgga
    20881 gagctacatc agcttcctgg ccctgctgag catcagcctc ggcgtgctga acctgttccc
    20941 cattcccgtg ctcgacggcg gtcatctggt gtattacctc atcgagatga tcaagggcag
    21001 cccggtgtcg gagcgggcgc agatgctggg ctaccaggtc ggtctcgtga tcctggtagg
    21061 cgtgatgctc ctggcgttct acaacgacat caaccggctg gccgccaact gatcgcggcc
    21121 ccggtccaaa tcccagcaat aggaacttcc agaactcccc atgaagcgct tcctgatcct
    21181 gcttcccctg ttgctggggc tcgcccgggc cgtgttcgcc gagagcttcg tcgtcaccga
    21241 catccgcatc gagggcctgc agaggatctc gcccggcagc gtgttcaacg acatcccgat
    21301 caaggtgaac gaccgcatcg accaggccgc gatcgcgcag acctcgcgcg cgctgttcaa
    21361 gtccggcaac ttcgacgacg tgcagatcgg ccgcgacggc gacgtgctgg tgatcacgct
    21421 ggtggagcgc ccgtcgatca gcgagatcaa catcgacggc aacaaggcga tcaagaccga
    21481 ggacctgctc gaggggctga agggctccgg gctcgcggtg ggacaggtgt tccagcgcgc
    21541 cacgctggag cagatgcgcg tcgagctgca gcgccagtac gtgagccagg gccgctatga
    21601 cgccacgatc gagaccgagg tggccgagca ggcgcgcaac cgcgtcgcgg tcaacatcga
    21661 catcaacgaa ggcagcgtcg ccaggatcaa gcacatcaat atcgtcggta acacggtgta
    21721 cggcgatcgc gacctggtgg atcttttcga gctgaagacc accggctggt tctcgtggct
    21781 cagcggcaac gacaagtact cgcgcgagaa gctcaacggc ggcctggaga gcctcagctc
    21841 ctactacctc gaccgcggct acatccgttt caacatcgat tctacccagg tcgcgatcac
    21901 gccggaccgc agcgcggtct acatcaccgt caacgtgacc gagggcgaga agtacaaggt
    21961 caccaaggtc gatctctcgg gcgacatcgt gattccggag tcggagatgc gcggtttcct
    22021 gctgctgcgc gacgggcagg acttctcgca ggtacaggtg accaacaccg aggagatgat
    22081 cacgcagcgc ctcggcgtcg agggttacac cttcgccaag gtcaacggca tccccgagat
    22141 cgacgacgag aacaagacgg tcgcgctgaa gttcttcgtc gacccgggca agcggaccta
    22201 cgtgcgccgc atcgagttcc gcggcaacac caagtcggtc gacgaggtgc tgcgccgcga
    22261 gatgcgccag atggagtcgg cgccggcgtc ttcggcgcag atcgagcagt cgcgcgtgcg
    22321 cgtcgagcgc ctcggcttct tcaaggaagt gaagaccgag aacgtcccgg tgcccggcac
    22381 cgacgacatg atcgacgtgc agtacacggt cgaggagcag tcctcgggct cgatcggggc
    22441 gagcgtcggc tacgcgcagg acgccggcct gatcctcggc gcgaacctgc agcagaacaa
    22501 cttcctcggg accggcaagc aggtcggcat cgggctgaat tccagcaagt accaggatct
    22561 ctacagcttc aactacgtga acccgtacta cactccggac ggcgtgagcc gcggtttctc
    22621 ggtgttctac cgttccaccg acctcgagga gatcaacgtc gcgagctaca ccaccgacac
    22681 catcggcggc acgatgagct tcggctaccc gatcagcgag acgcagcgtc tcggcctcag
    22741 cctcggcgtc aaccgcaccg acatcagtgc cggtatcggc gcggtgcagg agatctcggg
    22801 cagcccgcgc ccgatagacg atgccatcgg ctacaccagg caggacgagc tgaacgattt
    22861 cggctatccc gacctggaga acatcagggc gccggttccg gacgaattct tcaagagcga
    22921 tattccgacc ggattcctcg acatctacgg cgacagcttc gataatttcc tggtgaccgg
    22981 cagctggtcg cagttcgcgc tgaaccgcgg ccagctggcg acccgaggct actcgcagaa
    23041 cctctcgctg caggtggcaa ctcccggcag cgatctcgag tactacaagc tcacctacaa
    23101 cggccaggtc ttcatcccgc tgtggcgcca gttcaccttg cgcctgcgca gcgatctcgg
    23161 ctacggcgac ggctggggcg acaccgatga gctgccgttc ttcgagaact tcttcgcggg
    23221 cggcttccgc tcggtgcgcg gcttcaaggc caacacgctg gggccgcaga gcacgccggc
    23281 ggtgcgttac cagatcctta atttgccggt gtacgacgcg cagggcaaca tcgtcgacct
    23341 cgaggacaag tacgtctaca tcacctgcct cgggccgacg tacgacgcca agggcaattt
    23401 cctcggctgt accaactcag gcgaggagaa gatcattcac aacccggtct acagcggcaa
    23461 cagcgagaac cgcccgttcg gtggcgacgt cctcgtcgag ggcagcgccg aggtcctgtt
    23521 cccgctgccg ttcatcaagg accagcgctc ggtgcgcacc ggcgtgttcc tcgatttcgg
    23581 taatgttttt gataccaatt gcagtgccac gcaagtagta tgctccgacc ttgatttcgg
    23641 cgaattgcgg tattcggtag gctttggagt cacgtggatc accggcttcg gacccatcac
    23701 gttcagtctt gccaagccgc tcaacgacga gcgaacggac aagaccgaaa tcttccagtt
    23761 ctcgctgggt cagggttttt gacgaatttt ccccgaatca catccgcatc tttggagtcc
    23821 tgacgtgaag cgctttggtc gtgctgccgt aattgcctgc ctgatgaccc ttgctcccgg
    23881 tcttgcgttc gcgcagggca aggttgccgt gctcaacctc gaggcggcgg tgttcaacac
    23941 cgaggaggcg aagaagcaga tcgaggcgtt gcgccagacg ccggagtacc tcgcgaacaa
    24001 gaaggaggcc gaaggcctca agaagcagta cgaggacctg gttgcgcagt tcaacaagaa
    24061 ccgcgaggtc atgagcgccg agcaggaaga agagcagggg cgcaagatca agcagatcgg
    24121 caccgacctc gaattcgtgg ccaagaaact gcagcagtcc gagcgcgaag tggcgcagcg
    24181 catcatgcgc gagatgatgc cgcgcgcgaa caccgtcgtg atggacctgc tcaaggccga
    24241 gggcgtcggc ctgctgctga acgcgcaggc ggcgctgttc gccgagcccg gcttcagcct
    24301 cgatgccaag gtcaccgaaa agctgaacca gaccaagaag tgacgcgacc ccgggacggc
    24361 tgagtcccgg gctcacatcc ggccgcggcg ccacgccgct gcggcctgcc ggctcctgcg
    24421 cgcccccgcc gccattgcgc tcgcgcgggt gggggccgat catctcgata aggaggccgc
    24481 cggatgctga tggacgtcaa cgaggttcgc gaatacctgc cgcaccgtta cccgttcctg
    24541 ctggtggatc gcgtgctgga actggacccc ggcaagtcga tcgtcgcgta caagaacgtg
    24601 agcgtgaacg aaggcttctt ccagggccat ttccccgatc tcccggtgat gcccggcgtg
    24661 ctgatcgtcg aggccatggc gcaggcggcg ggcatcctcg gcttccggac catgaacaag
    24721 aagccggccg acggctcgat ctactacttc gtcggcgccg acgacctgcg cttcaagcgt
    24781 cccgtggtgc cgggcgaccg gctgcagctc gaagcctcga tactcaccga gaagcgcggc
    24841 atctggaagt tctcctgccg cgccagcgtc gatggtcaga ccgcctgcga ggccacgata
    24901 ctctgcgcgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgcgcca
    25021 gcgtcgatgg tcagaccgcc tgcgaggcca cgatactctg cgcggatcgc aagcgctgat
    25081 cgcgcgcttg ctgccggaac cgcgcccgtg acggactggc gcggggtgct ggcctcgggc
    25141 gtgctgctcg ccggcgtcga cgaggccggg cgcggtccgc tcgccggcga cgtgatcgcc
    25201 ggcgcggtga tcctcgatcc ggcacgcccg gtgcccggtc tgcgcgattc caagaaagtc
    25261 tcccgtgcgc gccgcccgca actggccgcg gccgtgcgcg aggcagcgca ggcgtgggcg
    25321 atcgggcggg ccagcgtcgc ggagatcgat cgcctgaaca tcctgcaggc cagcctgctc
    25381 gcgatgcggc gcgccgtgga ggcgctcgcg ctcgcgccgc agttcgtgct ggtggatggc
    25441 aaccgctgcc cggcgtgggc atatcccgcg ctggccgtcg tgggcggcga tgacctggtg
    25501 ccggcgatca gcgctgcctc ggtgctggcc aaggtggagc gcgacgcgga gatggtcgcg
    25561 ctggatgcgc tgtatccggg ctacggcttt gccgcgcaca agggctaccc gaccgcggag
    25621 cacctggcgg cgctgcgccg cctcggtgcg agtccggtgc accggcgctc ctttgcgccg
    25681 gtgcgggccg cggttgcaca atgcgcgctg gaccaggaat gaggctgcca tgagcgcggg
    25741 tttcgtacat ctgaggctgc attcggaata ttcgctgatc gacgggctgg tgcgtgtcga
    25801 tgaactggtc gagcgcgcag tcgcgctcgg catgcccgcg gtcgcgctca ccgaccacac
    25861 caacctgttc gcgctgatca agttctacaa ggaggcgcag tcggccggca tcaagccgat
    25921 cgtcggctgc gacgtgcgcg tggagcacga ggggcagacc catgcgctca cgctgctggt
    25981 tggcgcgcag gagggttacc gcaacctgac ccggctgatc tccaggggct ggctcgaagg
    26041 ccagcaactc gggcggccgg tgctcgcgcg cgcgtgggtg gaggagtttt ccggcgggct
    26101 gatcgcgctc tcgggcggtc gcgagggcga catcggccag gcgctgctcg ggggcagggc
    26161 ggagcaggcc tcgcagctgc tcgattactg gatggcgctg ttccccgagc gtttctatct
    26221 cgaggtgagc cgcaccgggc gtgcgcagga agaggattac ctgcacctgg cagtggcgct
    26281 cgccgggcgc cacggttgcc cggtggtggc gaccaacgac gtgcgcttcc tcgatcgcag
    26341 cgactacgag gcccacgagg cgcgggtgtg catccacgag ggccgcacgc tgaacgaccc
    26401 gcgccgccag cgccagtaca gcccgtacca gtacctgcgc agcgccgcgg agatggccga
    26461 gctgttcgcg gacctgcccg aggcgctgga gaacagcgtc gagattgccc ggcgctgcaa
    26521 catcggcatc gagctcggca aggcctacct gcccgactac ccggtgccgg cgggcatgga
    26581 catcgccacc ttcctcgggc gcgcggccca cgacgggctc gaggagcggc tcgcgcgcgt
    26641 cgcggatcgc gaggcgcgcg agccggagta ccgcgcgcgg ctcgatttcg agctgcagac
    26701 catcaactcc atgggcttcg ccggctactt cctgatcgtg atggacttca tccgctgggc
    26761 caagaacaac ggcgtgccgg tggggccggg gcgcggctcg ggagcgggtt cgctggtggc
    26821 ctacgcgctc ggcatcaccg acctcgatcc gctgcagtac cagttgctgt tcgagcgctt
    26881 cctgaacccc gagcgggtgt cgatgccgga cttcgacgtc gatttctgca tggaggggcg
    26941 cgaccgcgtg atcgcctatg tggccgacac ctacgggcgc gaggcggtgt cgcagatcat
    27001 caccttcggc accatggcgg cgaaggccgt ggtgcgcgac gtggcgcgcg tgcagggcaa
    27061 gccctacggc atggccgaca agctctcgaa gctgatcccc ttcgaggtcg gcatgacgct
    27121 cgagaaggcg atggcgcagg agcaggcgtt gcgcgacttc gtcgagcaga acgaggacgt
    27181 tgccgagatc atggaaatgg ccttcaagct ggagggtctc gcgcgcaacg tcggcaagca
    27241 cgccggtggc gtggtgatcg cgccgaccac gctgaccgat ttcgcgccgc tgtacgcgga
    27301 cgattccggc ggcggcctgg tgacgcagtt cgacaaggac gacgtcgaga aggtcgggct
    27361 ggtgaagttc gacttcctcg ggctgcgcac gctcacgatc atcgactggg cgctggcgat
    27421 catcaaccgg cgtcttgccg tcgagggccg tgcgcccatc gacatcacga cgatcccgct
    27481 cgacgacgcc agcgtctatg cgctgctgac gcgcgccgag acgaccgcgg tgttccagct
    27541 cgaatcgcgc ggcatgaagg acctgatcaa gcgcctcaag cccagctgct tcgaggacat
    27601 cgtggcgctg gtggcgctgt tccgcccggg gccgctgcag tcgggcatgg tcgatgactt
    27661 catcgaccgc aagcacggca agcaggcggt gcgctacccg cacccgtggc tggaaccgat
    27721 cctcgagaac acctacggcg tcatcctgta ccaggagcag gtgatgcaga tcgcgcaggt
    27781 gctcgccggc tacacgctgg gcggtgccga catgctgcgc cgcgcgatgg gcaagaagaa
    27841 gcccgaggaa atggccgagc agcgcgagat attcctcgcc ggcgcgcgca ccaacgatat
    27901 cgaccccgcc gtcgccggct cgatattcga cctgatggag aagttcgcgg gctacggctt
    27961 caacaagtcg cactcggccg cctacgcact ggtgtcgtac cagacggcct ggctcaagcg
    28021 gcatttcccg gccgagttca tggccgcggt gctgtcctcg gaaatgcaca acaccgacaa
    28081 ggtcgtggtg ctgatcgagg agtgccggca catgaagctg ccgctggtgc tgccggacgt
    28141 gaacgccagc gagttccgct tcaccgtcaa cgcgcgcggc gaggtggtct atggcctcgg
    28201 cgcgatcaag ggcatcggcg aggggccggt cgagagcctc gtcgcggcgc gcgaggctgg
    28261 cggtccgttc cgcgacctgt tcgatttctg ccgccgcacc gatccgcgca aggtcaacaa
    28321 gcgcgcgctc gaggcgctga tccgttccgg cgcgctggat ggactcggcg cgtccaggcc
    28381 ggtactgatg gccgcgatgg gcgaggcgct gaaggcggcc gagcagcagg cgcgcaacca
    28441 ggacgtcggc ctgctcgaca tgttcgccga cacggcggcg cccgcgctcg ccgcggacgt
    28501 ttacgccgat ttcgcgcagg cgccggcgtg gagcgcgcgc gagcggctgc gcggcgagaa
    28561 ggagacgctc gggctctacg tgaccgggca tcccatcgac gaccacgagg ccgagatctc
    28621 gcagttcatc cgcacgcgaa tcgtgcagtt gcgcgccgac caggagccgc agcgcatcgt
    28681 ggggctggtg gtcggcatgc gcacgatgaa gagcaagcgc ggtgacgaca tcgccttcgt
    28741 gcagctcgac gaccgcaccg cgcgcatcga ggtgacgctg ttcgccgata cctaccgttc
    28801 ctgccgcgag cacgtggtga aggacaactt cctcgtcgtc gagggcgtgg tcagcctcga
    28861 cgatttctcg ggggcgctgc gcatgcgcgc caacgaggcc tacacgctgg gcgaggcgcg
    28921 ccggcgcttc gcccgcgagc tggtgctgca actcggagcg gaggatttcg gacccgattt
    28981 cccggaggcg ctggggcgtc tgctcggcgg tgggcgctcg cgcgagggct gcgcggtgcg
    29041 ggtgctctac agcgcccccg atgcgagggc cgagatcagt ctcggggccg agtggcgact
    29101 gtcgcccagc gacgaggcgc tggggcgcct gcgcgagcgc ttcggccccg agcgcgtgcg
    29161 gctgcgctac gcctgagcgg gtttgctgcg catggaagtt ttgcggtcgc cttgctaagc
    29221 ttgctctccc gattttgcgt gctccaggat cccggccctc gatgaatccc aactttctcg
    29281 atttcgaaca gcccatcgcc gagctcgagg caaaaatcga ggaactgcgg ctggtaggca
    29341 acgacaacga gatcaacatc accgaggaaa tcgagaagct ccggctcaag agcgaccgcc
    29401 tcacggagaa gatctactcg ggcctgacgc cgtggcagat cgtgaaggtg gcccgtcacc
    29461 cgcgccgccc ctacacgctc gactatatcg agcgcatttt caccgatttc gacgaactgc
    29521 acggcgaccg ccatttcgcc gacgacaacg cgatcgtggg cggcatggcg cgcctggatg
    29581 gtatcccggt catggtcatc ggccaggaga agggacgcag cgtgccggag aaggtgctgc
    29641 gcaatttcgg catgcccaag cccgagggct accgcaaggc gatgcggttg atgcagcttg
    29701 ccgagcgctt tgcgctgccc gtgatcacgc tgatcgacac tcccggcgcc tatcccggca
    29761 tcgactccga ggaacggggc atcagcgagg cgatcgcgca gaacctggcc gtgatgtcgc
    29821 gcctgcgcac cccgatcatc tgcgtcgtga tcggcgaggg cagctcgggc ggggctatcg
    29881 gcatcggtgt gggcgaccac ctggcgatgc tgcaatacgc cacctacttc gtgatctccc
    29941 cggaaggctg cgcgaacatc atctggaaga cctccgagaa ggcgcccgag gccgcggagg
    30001 ccatgggggt tacctccggc gtgctgcagg agctcggcat cgtcgatgcg accatccccg
    30061 agccgctcgg cggcgcgcac cgcgacgtcg acgccatggc cacgcgcatc cgcgagcacc
    30121 tggctaccga gctcgcgcgc ctcaaggccg tgccgctcga ggaattgcgt gaaacgcgtt
    30181 tccagcgcct gatgtcctac ggcacccgct gaccgcgatg ccgtgatcac ccccgccctc
    30241 tgcctgccgc tgctggagca gttcccggcg gcgaggcgct gggtgatcgc ctacagcggc
    30301 ggtctggact cccatgtact gctgcacctg tgcgcgcggt tgcgcgagct gcgccccggc
    30361 gtgccgccgg tggtggccct tcacgtcaac cacggcgtgc agcggcaagc cgatgcgtgg
    30421 acggcacact gcgccggtgt gtgcggcgcg ctggggattc ccttcttcgc gcggcgtgcg
    30481 gagctgcttg cgaccgacgc cgcgcagccc tccgaggatg cgttgcgggc ggcgcgctac
    30541 cgggtcttcg agcagttctg cgccggggac gacctgctgc tgctcgcgca ccagcgcgag
    30601 gaccaggcgg agaccgtgct gctgcgcctg ttgcgcggcg cgggcgtggc cggtctcgcc
    30661 ggcatgccgc gcgagcgtgc ctgcggccgc gcgcggctgt gccggccgct gctggagacc
    30721 gggcgcgcgc agctgcgcgc ctatgccgag gccgagggct tgcgctgggt cgaggatccc
    30781 agcaaccact gtcccgacta cgaccgcaat tacctgcgcc tcggcgtgat gccgttgctc
    30841 gccgcgcgct ggcccggcgc cgtcatgagc gtggcgcgcg ccgcccacag cctggggcag
    30901 gccgccgcga tctgtgccga acgcgccgac gaggacctgc gcgagtgcgc cggcagcgat
    30961 cgctacggcc aggccttcct ggcgctcgcg acgtggcgcg cgctcggcgc ggcgcgcgcc
    31021 acgtcggcac tgcgcgcctg gctcgcgcgt cgcggtgccg gctggccgga cgcacagacg
    31081 ctcgcgacgc tggaacgcga ggtgatcggt gcgcgccgcg atgcccagcc ccgcctggtg
    31141 ctcggcgcgg tggtggtgcg tcgctaccgc gagtgcctct acgcgctccc ggcgcagagc
    31201 gaagccgggg tgcccgtcgg ggccgccatc gtgccggata cgccactgtg cctgcccggt
    31261 gccgggcgca tcgtgctggg ctcgggccgc gggggaggcc tgcgtcccgg tcagacctac
    31321 cggatcggtt tcggggcgcc gggttggcat tgtcgtcccg ccgggcgccc gcgcaagacg
    31381 ctggcgcagc tggcacagga gcacggcgtg ccgccctggc tgcgcgctcg cctgccgctg
    31441 ctgtatgtcg atggggaact cgccgcggtg gcggatctgt gcgtgtgcga gggttttgtg
    31501 gcgccgccgg gtgccgcgtc gctacgcctg gactggcgcc cgggctgaga ctttcaccgc
    31561 gctggtgcgg gcgcggcctt gcggctgcgg cgggcgtagt acgattgcga ggctccgggc
    31621 gcgcggcggc tcaccacttg aggatgtcga gcgccttggc gatctccggg cgcagggtca
    31681 gcacggtatc ggcgttcacc gagccgatgg tgatgtcttc ctcgaagaac agcgcggcgc
    31741 cggggtagtg gtccttcttg cggctgcccg ggttgtaagc cagttcgtcg cagacccgcc
    31801 gcgcgacctc ggcgtactcc gcgaacgcgc cctcggcgct ggcgtagtcg ccgacgcggc
    31861 tgatggcctt gacgctcagg cgctgccccg gtgccttgcc gtggatcgcg agcatcattt
    31921 tctgcgtgcg tgacagcttg ggttcgatgc cgatgatcgc atcggcgatc tcgcgcgcgg
    31981 caggtttctt cttgccgcgt tgcctgcgtt tttcctgctg tatttcgtcg acgatggcgc
    32041 gcagcgcgtc gatgatctcc tcgaggtcgg tgccctcgta atcggcgatg cgctccttgt
    32101 tcttccaggc acgcccgcgg tattcatcgt tccagaagcg ggcgatcagg tcgtaattgc
    32161 agtaatcctc tttcagaacg cgtttgtcgg attcgacgag gggcaattgc ggcatccggg
    32221 acctctgttg tcgggcatct ccgccgcagc cgctgattgg gtggacaggg cgcggctgtg
    32281 ggctcctggc gcgaggcgcc gcattctttc catttggaaa ttcaaatgtc aagaatattc
    32341 ttgcgcactc ctgaacggct cgacggccgc ctgtcggggc gtctgccagc cttgccgcag
    32401 tggggggggt tcgagcataa tgccgcgctc gtcaggacgg cggccccctg tcaatggtgg
    32461 ccccggtcgg tcccctcgca acgctggacg atgaatcccg ccaggcccgg aagggagcaa
    32521 cggtagtcgt tgacgcgtgt gccggggtgt ggctggccgg ggctgccacc cttagcggac
    32581 ggggcgggta taatcccgcc cctccacgca atccaggacc ctcaggtcgc aatgtcatat
    32641 caggtactcg cgcgacgctg gcgcccgcag accttcgcgc agatggtcgg tcaggaacac
    32701 gtgctgcggg cgctggtcaa tgcgctcgaa cgcaaccgcc tgcaccacgc ctatctcctc
    32761 accggaaccc gtggtgtcgg caagaccacg ctggcgcggc tgatcgccaa gtgcctgaac
    32821 tgcgagcagg gtatctcggc ctcgccctgc ggcgagtgtt cggcctgccg ggagattgcc
    32881 gagggccgct tcgtcgacct gctcgagatc gacgcggcct cgcgcaccaa ggtcgaggac
    32941 acccgcgacc tgctcgagaa cgtgcagtac gcgccggcgc gcgggcgctt caaggtctac
    33001 ctgatcgacg aagtgcacat gctctcgacg cactccttca atgcgttgct caagacgctg
    33061 gaagagccgc cgccgcacgt gaagttcctg ctggcgacca ccgacccgca gaagctgccg
    33121 gccaccgtgc tctcgcgctg cctgcagttg cacctgaaga acctgagccc cgagcagatt
    33181 tccgcgcacc tcgcgcatgt gctgcgcgag gaaggcatcg gcttcgagga tcctgcgctc
    33241 tgggagcttg cggtggcggc cgaaggcagc atgcgcgacg cgctgagcct caccgaccag
    33301 gcgatcgcgc atggcggcgg cagcgtggcc agcgcgacgg tgaccgagat gctcggttac
    33361 atcgagcgtg aactgctgcg cgcgctggtc gaggcgctgc tgcaggcctc ggccgagaac
    33421 gtgcttgccg tggtcgccga tatggcggcg cacggcgccg acttcgagcg cgcactggcc
    33481 gaactgctgg cggtgctgca ccgcgtggcg ctggcgcaga cggtgccggc gacgctcgca
    33541 gccatgcacg ccgagcgcga gcgcttcgcg cactgggccg cgctcgcctc gcccgccgat
    33601 gtgcagctgt attaccagat cggattgatg tgccgtcgtg acctgcccta tgcgccggat
    33661 gcgcgcagcg ggttcgaaat ggccctgctg cggatgctcg ctttccgccc ggcgcagccg
    33721 gcggatgcgc gcagcgccgc cgcgccgccc tcgccgcgcg ccacggtgcc ccgcaacgaa
    33781 cccgccgcgc agggggcggt ggccccgcca aaaaagcctg aaccggcggc ttgcgggccg
    33841 gccggtgttc acgctgcccc ccggccttcg gctccgaccg agccgggccc cgcggtggcg
    33901 ccgccggccg cgcttggcga ggctcctgca agcgcgggag ctccggcggc ggagcccgcg
    33961 acgccggcag ccggcgtggt gtctggcgcg gcgccgacgc aggcaatccg gcagggcgat
    34021 accgcgcccg ccgctcggtt ggccgatgtg aacccgggca actggcaccg gctgctggcg
    34081 gagtgcgagc tgggcggcat ggcaggcacg gtggcctcgc actgcctgcc ggaggcggtg
    34141 gagggcgcga cgctgcaact gctgttgcag caggagcacg agatcctgta ccagccgcag
    34201 tgcgacgagc gcatcgccgc ggcgctggcg agccgcttcg ggcacgcggt gaccgtgcgc
    34261 gtgcgcccgg cgcgggtcga gggcgagacg ccggcggagc gcgccgcgcg cctcgccgcc
    34321 gagcgccagc agcaggcggt cgccgcgatc gaagccgacg ccaacgtgcg catgttgatg
    34381 gagaattttt ccgggaaact gcaccgcgac tcgatcacgg tgccggagcc cgcagacacc
    34441 gggagggaag gggcgtgaaa ggcctgaacg atttgctcaa gcaggcgcag caactgcagg
    34501 aacaggtgaa gaacgcgcag gaggacatcg cgcgccagga agtgcagggc gaggccggcg
    34561 gcgggctggt gcgcgtcacg atgacggggc gtcacgacgt gcgccgggtg catatcgagc
    34621 cttcgctgct cggcgaggat cgcgaagtga tcgaggacct cgtcgcggcg gcggtcaacg
    34681 acgcggtgcg ccgcgtcgag gcgctgcagc gcgagaagct ggcgggcgct gcgggtgggc
    34741 tgtcgctgcc cgaaggcttc aagctgccgt tctgagcacg gggattcgag gcgatgcgat
    34801 tcggtccgtt gatcgaccag ctgatggagg ccttccagtg cctgcccggc gtgggtccga
    34861 agtccgcgca gcgcatgacg ctgcacctgc tcgagcgtga ccgcgccggt ggcgagctcc
    34921 tggcccaggc gctggaacgg gcgctggcgc gcgtgggccg ctgtgcgcgc tgccgcaatc
    34981 tcagcgaggc gcaattgtgc ccgatctgcg ccagcgatcg gcgcgaccgc ggcgtgctgt
    35041 gcgtcgtcga gacgccggcc gatgtgctgg cggtggagca ggccggcggt tacgccgggc
    35101 tctacttcgt gctgctgggg aggttgtcgc cgatcgatgg catcgggccg gccgagctcg
    35161 ggctcgacct gctggcgcag cgcgtcaggg acgagggcgt gaaggaagtg atcctcgcca
    35221 ccaaccccac ggtggagggc gaggcaaccg cctatttcat caccgagcag ctgcgcggag
    35281 ccggggtcgc ggtgtcgcgc atagcgcacg gcgtgccgct cggtggcgaa ctcgaatatg
    35341 tcgatggcgg cacgctgcag cacgccttcc agggacgcaa gccggtatga gttcaggcat
    35401 cgtggaaacc ttcaacggca tgcccgtgca cttcatcgac gcgcaggacg cactcgagac
    35461 tgcggccgcg cgttggctgc gccgggaggt ggtggcgctg gacaccgagt tcatgcgcgt
    35521 gcgcaccttc cacgcgcagg cggcgctgtt ccaggtctgc gacggtgaag cctgctacct
    35581 ggtcgatccg ctcgccgtcg ctgacctggc gccgctggcg cgcctgctcg atgcgcgcga
    35641 cgtgctcaag gtcatgcatt cctgctccga ggacctcgag gtctgcgagc gccacctcgg
    35701 cgcgctgccc gaaccgctgg tcgacacgca gatcgtcgcg gcattctgcg gttacccgct
    35761 gtcggtcggt taccagaaga tgctcgccac ggaattcgac gtgcacctcg gcaagtccga
    35821 gacgcgcaca gactggctgc agcggccgct gtcgccagcc cagatccact acgccgccga
    35881 ggacgttgcc ttcctgcccg cgctgtggcg cgtgctcgcg cagtgcctgg cgggcaagcc
    35941 ggccggcgac tgggcgcgcg aggaatgcgc cgcgaccgtg gcccgcttcc gcgcgcgcac
    36001 cggcgcggac tggcgcgcgc tcggtggtgc ctggcggctg gaccggcgcg ggctcgccgt
    36061 gctgagcgcg ctgcatgcgt ggcgcgagga gaccgtgcgc acgcgcgacc tgccgcgcag
    36121 ctggctggtg ccggacgcgg cgctgctgcg gctggcggaa ctgcggccgc agcgcgagga
    36181 acagctcgtc gcggtcgagg acctgcccga gggcgtgcgg cgtcgctacg ggcgcgcaat
    36241 catcgagcag gtcgccacgg cgctcgcgct cgaggccggg gagttgccgc cggcagtgcc
    36301 gccgccgctg gataccgcgc aggggcgccg gcttaaggcg ctgcgcgcgc acgcgatcac
    36361 gcgtggcgag gcgctgggtg tcgcgcccga gatgctggcg cggcgacgag atttcgagga
    36421 gctgctgcgg tggtgcgacg ctggcgaggg cgcgcagccc cccgcggtgc tcggcggctg
    36481 gcggcgcgag gtgatcggcg aggaactgct cgcgctggcg cgcaaggggg cggcatgaaa
    36541 tccatctgcg cggtgtaccg ttcgcgccgc aatccggaca cctatctcta cgtcgatcac
    36601 cacgagggcc tgagccgtgt gcccgaggcc ctgcagcgcg agctcggcgg caccgagcgc
    36661 gcgatgacgc tggtgctgac gccgcagcgg cgcctggcgc gcaccagcgc ggccgaggtc
    36721 ctgcgcgcca tccgtgaaca gggctattat ctgcagatgc cgccattgcc ggaggggctg
    36781 cgggtgcagg gcagggacgc gacatgatga acgcatccga ctacgcgctc gcgtggtcgc
    36841 tctacctgct cgcgtgcgcg gtggtcctgt tcacggtgtg gggcctgagc gcgcgcttct
    36901 ggtcctggat caaggatccg ctgcgcgcgg tggcggcggt gttgctgctg acgccgggca
    36961 gcgtggatgc cgagggtaac ttcctcgcgc cggcgatctt catcgtggca ttcgagttgc
    37021 tcaccgtcga ggacggcggt atcgggccgc tgctcggcgt gcgcctgctg ctgttcgtga
    37081 tcggcgcggt gctcgccgcc tgggtgctgc gtgcactgtg gtactggctg gtggccagcc
    37141 ggcgtaccgg ggcggtgccg caggcgccgg ccaatcccgc gtctcgcaca gcgcagctgc
    37201 gcacggtcga catccgcgac cgcagcagcg gccgcgatcg ctcctgagcc gattcagctc
    37261 atcgcctgca ccagctcgcg ttcgctgaag cggccctcga acaacgggct gcgcaggtag
    37321 cgctcgcccg aatcgggcag tatcaccacg atcgtcttgc ccaggtgctg cggctggcgc
    37381 gccacccgcg ccgcggcggc gacggcggcg ccgcaggaaa tgccggcgag gatgccttcc
    37441 tcgcgcatca gccggtgcgc gtactccatg gcctcgtcgt cgctcaccgg ttccacctgg
    37501 tcgatcaggg agagatcgag gttctgcggc acgaaaccgg cgccgatgcc ctggatcttg
    37561 tgcggggcgt gggtcggcgt cctgcccgcg agcgtgtccc tgatcatcgt cgtggaggcc
    37621 ggttccacgg ccaccgccgt gatcgccttg cccatggtcg tcttgatgta gcgcgcgaca
    37681 ccggtgatcg tgccgccggt gcccacgccg gccacgaaca cgtcgatatt gccctcggtg
    37741 tcgcgccaga tctcggggcc ggtggtgcgc tcgtgaatcg cgggattggc gggattgcgg
    37801 aactgctgca gcatcaggtg gcgcgccggg tcgcgggcca cgatctcctc ggcctttgcg
    37861 atcgcgcctt tcatgcccag cgcgccgtcc gtgagcacca ggttggcgcc cagcgccgtc
    37921 atcagctggc gccgttcgag gctcatcgtg tgcggcatcg tcagggtgag ccggtagccg
    37981 cgcgcggccg ccacgaacgc gagcgcgatg ccggtgttgc cgctggtggg ttccacgagc
    38041 tcgatacccg gcttgaggct gccgtcctcc tcggccgccc agatcatgct cgcaccgatg
    38101 cggcacttca cggaaaaggc cgggttgcgg ctttcgagct tggcgtagac cttgccttcg
    38161 gcaacgcggt tcagacgtac cagcggggta ttgccgatcg agtagctgtt gtcggggaag
    38221 atgttcattg cggtgctcct ctgcccggaa tgtactgttt cggtccagcg accggggagc
    38281 tcaaggctac taccggcgcc ggggcaagtc cacgcgcgcc tgagcgcaat cgcggtgcgc
    38341 gatgagcgct ttggacagtc cgtcgccggc cggttttcgt ttagactgcg cgccgggaag
    38401 cgcgttgcgg cgcgcacgcg cagccactga tgcggagtcg gatcgaatga gtttcaaggg
    38461 taccgaacgt tacgtcgcca cggcggatct gcagatggcg gtcaatgccg cggtgacgct
    38521 gcagcgtccg ctgctgatca agggcgagcc gggaacgggc aagacgctgc ttgccgagga
    38581 ggtcgccatc gcgctgggca agccgctgat ccagtggcac atcaagtcga ccaccaaggc
    38641 gcagcagggg ctctacgaat acgacg
//
LOCUS       DAORUQ010000009        37398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_677_length_37398_cov_6.893394, whole genome shotgun sequence.
ACCESSION   DAORUQ010000009 DAORUQ010000000
VERSION     DAORUQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 37398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37398
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_37398_cov_6.893394"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="hemN"
                     /locus_tag="PLW13_01710"
     CDS             <1..911
                     /gene="hemN"
                     /locus_tag="PLW13_01710"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQY69112.1"
                     /translation="GTIALLKGLGFNRVSMGIQDFDPAVQRAVNRIQPFDMVENLVDA
                     VREHRFKSLSFDLIYGLPLQSPASMEQTLAKVLALDPDRVSCYSYAHLPEIFSSQRSI
                     DRMALPSADEKLEVLHTIIDTLTDAGYLYVGMDHFVKPQDELAQALHNGTLQRNFQGY
                     STQLAPELLGIGVSSISSTADLYCQNVKTVDEYYALLEQDKLPVQRGLRLTPEDQLRR
                     DVIMQLICRMGIDTGETGERHGIDFGSHFAREMRILDDMEREGLLASEGGQIRVTAAG
                     RPLVRNICMVFDAYLDPSRQRFSRTI"
     gene            1038..1793
                     /gene="fnr"
                     /locus_tag="PLW13_01715"
     CDS             1038..1793
                     /gene="fnr"
                     /locus_tag="PLW13_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HQY69113.1"
                     /translation="MLKSHDVNATGHRCPHDYQINCGNCRLNSICLPIALDIHEVDQL
                     EEIIQRSRPLQKGEHLYRQDQPFGSVFAVRSGSVKAYAITDNGQEQVTGFYFPGEVLG
                     MDGIAKNKYASSARAMETAAVCEIPFDKLGELSQRLPSLQRHFFQLMSQEIAEDQQLL
                     TLLSKNTADQRVATLMLSISARNKRRKLSAASFRLPMSRTDIGNYLGLTVETVSRVLS
                     RFQKLGVLSVDNKEINIIDAELLRSVADGSEAT"
     gene            1919..2509
                     /locus_tag="PLW13_01720"
     CDS             1919..2509
                     /locus_tag="PLW13_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HQY69114.1"
                     /translation="MITNAIGILFSPHSTWQRLAAVADRASNLLPILILAVLPAVGWY
                     YGTTHMGWTVGSGEVTKFARSGGIALGAALYVGLVVWVCIIGWSIHWMATSYGAQSTL
                     MRGITVAGFTAVPMLIAGVVGFNPVPAIVMVVGLGSVAWSLYLLYIGIPAVMKVPQDR
                     GFLFASAVVAVCLVILIAMMGAGVILWDMFQPEFSN"
     gene            complement(2544..3959)
                     /locus_tag="PLW13_01725"
     CDS             complement(2544..3959)
                     /locus_tag="PLW13_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY69115.1"
                     /translation="MPQVALVREHGVRGTRALIVEDDAELREALADTLELAGCRVMAA
                     PGAPEALALLAQNAVDVVVSDVNMSGMDGHELLRRIRALHPQVPVVLITAFGSIERSV
                     QAMRDGASDYLVKPFQPAQLLEVIARLGAGAAAGDEDPVAVARESVELLQLARRVAAT
                     DSTVLLCGESGTGKEVLARYIHRHSGRSEHAFVAINCAAIPENMLEAMLFGHEKGAFT
                     GAYTAMPGKFEQADGGTLLLDEISEMDASLQAKLLRVLQEREVERLGGRRPLAVDVRV
                     IATTNRELRAAVATGRFREDLYYRLSVFPLRCLPLRERGEDILPLAQRLVSRHAVKMH
                     HVPVQFDAGAVRAMLAHPWPGNVRELDNAMQRALILQDGVVVSAAHLRLEPVASSASL
                     GEPAPAAQYAPPEDADDQASGLNGDLRLREFELIVNMLKDTRGSKATAAERLGISPRT
                     LRYKLARMRESGLDVEAALGA"
     gene            complement(3934..5151)
                     /locus_tag="PLW13_01730"
     CDS             complement(3934..5151)
                     /locus_tag="PLW13_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY69116.1"
                     /translation="MSASLAQARDIVPGSVRDSAEIQAAFALFNQVSAELSDSYRLLE
                     RRVEELSSELEQVDRARLRELEGKERVSERLRSLLDLLPGGVVVLDRHGIVCDCNPAA
                     REMLQGGVLGERWVEVIRRCFAPRHDDGHEISLASGRRVSVATRSLERDKGQVVLLTD
                     QTETRELQDRLSRHQRLNAMGRMMAALAHQLRTPLAAAMLYASNLRDAQLTPEQTRKF
                     SAKILSRLTHLERQVRDMLIFVRGDVALENVSSLGELFEDIAAAMEAPLASAQARCEQ
                     RIDCDRTLQLLCNREVLSGALLNLVTNALQAAGQGALIAVQAALREGRVCISVCDNGP
                     GMSPEVQAQLGDGFFTTKPQGTGLGLAVVRAVARAHGGELEIRSVPGQGTCASVLLPL
                     HERGVDAAGRARA"
     gene            complement(5323..6762)
                     /locus_tag="PLW13_01735"
     CDS             complement(5323..6762)
                     /locus_tag="PLW13_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY69117.1"
                     /translation="MSNRTHILLLDHDAARRATASAILGFLDESHTALAALPAALAEP
                     PQVVLLAADGEGFDLEATLEALAALDPGVPVVITAAPADGVAPGRHEAMLIGTLGWPF
                     AYPRLVDALHRARVYREQYALGRGRGNGELKLFRELVGASAGLRAVRRMMSQVADTDA
                     TVLVLGESGTGKEVVARSLHAHSRRRSGPFVPLNCGAIPGELLESELFGHEKGSFTGA
                     VSSRAGRFELAQGGTLFLDEIGDMPLAMQVKLLRVLQERRFERVGGSQTLEADVRIVC
                     ATHKNLEEMIAKGSFREDLYYRINVFPIEMPPLRERSEDLPLLVNELTARLEREQRGS
                     VRFSAAALASLQRHPWAGNVRELANLVERMAIMHPDAVVGARDLPPKFRHLEAHEEDD
                     VILPAAPEPLETAAPASANASGLLPVNGLDLKEYIQDLEKSLIEQALDDCSGVVARAA
                     DRLRLRRTTLVEKMRKYDLLNPAAAAAEA"
     gene            complement(6895..7290)
                     /gene="fliS"
                     /locus_tag="PLW13_01740"
     CDS             complement(6895..7290)
                     /gene="fliS"
                     /locus_tag="PLW13_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HQY69118.1"
                     /translation="MYSKSALAQYQKINAQTGVVDADPHRLIQLLLDGAIDRIAQAKG
                     ALACGDAEAVGVALGKALGIISGLQGCLDKEAGGEIAANLDRLYDYMTLRLMDVHRGQ
                     SVEPLDEVGALLGTIRSGWEGIRGQAATS"
     gene            7571..9040
                     /locus_tag="PLW13_01745"
     CDS             7571..9040
                     /locus_tag="PLW13_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQY69119.1"
                     /translation="MPQIINTNIPSLNAQRNLDKSGQSLATSLQRLSSGLRINSARDD
                     AAGLAISNRFTSQIRGLNQAVRNANDGISLSQTAEGALAETGELLQRMRELSIQSANG
                     SQSGTERAALQAEVSQLQQEINRIAETTTFGGRKLLDGTFGTEAFQVGSNANETISIS
                     IANSRATSIGANTIASSGDINAAVAANGTVQPNNIDAQSLTLAGNLGSSTISIAAGAT
                     GDQIASQVEAVSANTGVSLTARTTATFAVGATGTLSFNIGSVSGSTSYSASVSVLVTD
                     VSDLSSLADAINASSAASGVTATANGATLSLVNEEGRDIFVGDFANSTAGNQTATLTG
                     ASGAAVTLTEGSTDSSTVGATLSFTSYQAFSATTTSATGGIFTALTNSSALSSVASID
                     IGTQQGAQDALAVVDAALQFIDATRGDLGAVQNRLLSTISNLQNVTENVTAARSRIQD
                     ADFAAETANLTRASILQQAGISVLAQANALPQQTLALLQ"
     gene            9132..9554
                     /locus_tag="PLW13_01750"
     CDS             9132..9554
                     /locus_tag="PLW13_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HQY69120.1"
                     /translation="MDTSRIAYGQAQPIAGRGKAVSADAQPNPAGAGSAGTATPAPAA
                     TPALSAADAAQAPLEDLQQLLEQASRHIHPEQRSLSFEIRDELGRAVVSVYDAETEEL
                     IRQIPSETAVRIATAMREMTQQGSGALPPSGLLLDEQA"
     gene            9592..11061
                     /locus_tag="PLW13_01755"
     CDS             9592..11061
                     /locus_tag="PLW13_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQY69121.1"
                     /translation="MPQIINTNIPSLNAQRNLDKSGQSLATSLQRLSSGLRINSARDD
                     AAGLAISNRFTSQIRGLNQAVRNANDGISLSQTAEGALAETGELLQRMRELSIQSANG
                     SQSGTERAALQAEVSQLQQEINRIAETTTFGGRKLLDGTFGTEAFQVGSNANETISIS
                     IANSRATSIGANTIASSGDINAAVAANGTVQPNNIDAQSLTLAGNLGSSTISIAAGAT
                     GDQIASQVEAVSANTGVSLTARTTATFAVGATGTLSFNIGSVSGSTSYSASVSVLVTD
                     VSDLSSLADAINASSAASGVTATANGATLSLVNEEGRDIFVGDFANSTAGNQTATLTG
                     ASGAAVTLTEGSTDSSTVGATLSFTSYQAFSATTTSATGGIFTALTNSSALSSVASID
                     IGTQQGAQDALAVVDAALQFIDATRGDLGAVQNRLLSTISNLQNVTENVTAARSRIQD
                     ADFAAETANLTRASILQQAGISVLAQANALPQQTLALLQ"
     gene            11177..12634
                     /gene="fliD"
                     /locus_tag="PLW13_01760"
     CDS             11177..12634
                     /gene="fliD"
                     /locus_tag="PLW13_01760"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HQY69122.1"
                     /translation="MASIGSLGVGSGLDLETLVTKLISAEGTPRSNSLASKEASIQAD
                     ISAFGSLKSALDKFRSSVATLGDANALQARTVTTGNSRYFSATAGERAVSGQYAVQVL
                     NTARAQKLVSTADFASASDTVGAGSLTISVGSASFQVTTTASTTIAELKTLINDAADN
                     AGLSASLLVVARDPQNAAAGTVARLALAADETGSANTIGISVSDADANNTDALGLSRF
                     YFSSADIANSQLGEQQAALDARIAVDGQTAFSNSNVFSDVIDGVTITALKDPADPLNP
                     DVETLGIALDNNGVAARISAFVTAYNEVVSAIRDVSAYNAEAGTAGALNGDATVRGIS
                     SRLRTIIGAAGIGDGTPRSLAELGIQTQRNGTLKLDTAKLDAALKDDFAGVGTLFTAP
                     DGVATRLESMLDGLLERGGTLATRTDGLDRQLKAIGTERDKLEIRLEKLEAQYRARFS
                     ALDSLVAGLQSSGDYLLQQLQNTASIIGRNPGGGN"
     gene            12634..12993
                     /locus_tag="PLW13_01765"
     CDS             12634..12993
                     /locus_tag="PLW13_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69123.1"
                     /translation="MQPDATGVRAALPARLRDAWDRIATIDAALAAALAGETPADVAQ
                     LGAQRTRCIEEFFDAFPLEAQTAGLRTEALRHLLAMNNALAAAAQRELATASEVATAA
                     RHQRKAISVYHEVQPKA"
     gene            complement(13061..14281)
                     /gene="flgL"
                     /locus_tag="PLW13_01770"
     CDS             complement(13061..14281)
                     /gene="flgL"
                     /locus_tag="PLW13_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HQY69124.1"
                     /translation="MHIFNTGVRHITNAQAEVARAQEQISSGKRVLTPADDPAAAALA
                     LGIRQEQARSAQYQRNADLAESDLQQQDAQLEAVENVMLQLREFALQAGNATLSANER
                     ATLVAGIEQAGQQLLSIFNTRSAYGDYLFAGFSGDTQPFVQSAAGRIDYAGDDGERSV
                     AIGAGLQVQSRNSGRELFVDIPAAASTFVTRNAALNSGTGDISIGRIVDQAAYDAVYP
                     DDLVVRFGVAPGTYQVDRIDRATGAASVVLPPQPYVDGAPLTITVAGAEVRITGSPAP
                     GDEFVLEASATQSLLLTVSRLADGLRNIPDTEAGAIERQRVVAEALNGIDRSEQSVTA
                     SRASLGPRLNLLDDARAVQSALDLINDEALSDLVDLDFDEAASRLSFQSFVLEAAQQS
                     LAKIANLSLFNFLR"
     gene            complement(14307..16313)
                     /gene="flgK"
                     /locus_tag="PLW13_01775"
     CDS             complement(14307..16313)
                     /gene="flgK"
                     /locus_tag="PLW13_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HQY69125.1"
                     /translation="MADLLRIATTGLLAYQGSLNTTGHNIANAGVEGYSRQRAELVSL
                     PGQNLSGAVFGNGVDIATVSRIVDQFVTSQLRTDTSLYFRAEALNGWNEQVDALFADS
                     SIGISDRIDAFFGVLQDAASDPQSTAVRTVVLDSATTLTQRFQSLYAQLEDMNDGLNT
                     QLTALAKEITALASGVAELNRQIAMRVGSSGAGGHQPNDLLDQRDRLIERLAGLVDVQ
                     AVADGSAINLYLGKGHALVTGTQASAAVAVPDRFDPGKLQLALELNGATSVVSYAATE
                     GQVGGLLAFRDELLYPAFNALGRVALALTDDINAQNRLGVDLDGDLGGLVFSDVNEQI
                     AAVLRARPASDNDPASTGGVRVFIEDTAKLTTSDYRLDIGSDGASWRLVRLQDATLVA
                     SGASLGDTLVTVDGFTLDLNLSEVPPGNFVAGDSFLIEPTRRGAQFVRTVMSRAEDLA
                     LAAPVRAAALTGNLGTGSIAPGETFDTGTAIFATPGQLSPPLLIRFSSATQFDVLDAN
                     TSAVLVAGLGFIPGTANTLFSRDSSDPDYFSFQVALAGEPAAGDAFRIDYNGGGTSDN
                     RNALLMAALQSADTMGNGSASYQGAYRDLVAATGSLTQRSRIDMQSSLAVLDQTRERR
                     ESLSGVNLDEEAANLIRFEQAYNASAQVISAARAVLDALFDALG"
     gene            complement(16317..17321)
                     /gene="flgJ"
                     /locus_tag="PLW13_01780"
     CDS             complement(16317..17321)
                     /gene="flgJ"
                     /locus_tag="PLW13_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HQY69126.1"
                     /translation="MSVAGAPAQTAAPAAWTDLAGLHSITTLGRADRGAALQAAARQF
                     ESFFVSQLLKGMRSANEIFAADNPMNTNEMQFRQDMLDQQLGVSLAQGRGLGLAEMLG
                     RQLRQQFGTQESAVAGAGTSVEAQLEARRIDAAPREPAPALLPRVARAALPPVVSSAL
                     QILEPVAQQAREKIAAGLFSGKEAFVRAVLPEARRAAQALGIDHRVLIAQSALETGWG
                     QSILSDHAGRSSHNLFNIKAGSYWEGRSVGVTTLEFSNGIPQQQRARFRAYGSFAESF
                     ADYIALLKGQPRYAAALANADDPARFMRSLQQAGYATDPAYAHKVMQLLADDAIAGAG
                     "
     gene            complement(17355..18509)
                     /locus_tag="PLW13_01785"
     CDS             complement(17355..18509)
                     /locus_tag="PLW13_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HQY69127.1"
                     /translation="MSMAIPLLRLRALLARRASRLGAAALVLLSCAGAAHAERLKDIA
                     ALAGVRNNYLIGYGLVVGLDETGDQTTQAPFTSQSLMRMLRQFGIALPEGMRLQLKNI
                     AAVAVHAQLPPFAKPGQTIDVTVSSLANAKSLRGGTLLLTPLKGVDGEVYALAQGDLV
                     VGGFGAEGQDGSRVTVNVVNAGRIANGAIVERGVETSFTQGSELQFNLLRPDFTTAKH
                     VAERINGMLGSDVANALDAGTVHVRGPQDADQRVSFVSLLENLEVEPGEAPARVVINS
                     RTGTIVVGNHVTVEPVAVTHGSMTVVVTENLQVSQPGPFGGLGRTVVTPQSDVQIDQE
                     GNRMWKLAGVTTLDQLVEAVNSVGAAPGDLMAILEALKQSGALRADLIVI"
     gene            complement(18506..19219)
                     /gene="flgH"
                     /locus_tag="PLW13_01790"
     CDS             complement(18506..19219)
                     /gene="flgH"
                     /locus_tag="PLW13_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HQY69128.1"
                     /translation="MTRFIDSTPAPRRAGIMLVALLASVLAGCAAPRLQEMPDDPAYA
                     PLTANTPAPDFHAQGAIQYARFGSSLFSDRRAAQVGDIITVRLIERTQASKDADTAVS
                     KQSDLQFNAGTMLGNDFAGGDHSLETDVQQNRDFQGNAESSQSNSLSGSIAVTVTEVT
                     PNGLLRVRGEKWLQLNRGQEYIRLSGLVRQEDLAPDNSVPSTRLADARIGYSGTGELA
                     QSNNMGWLGKFFNSGVWPL"
     gene            complement(19232..20017)
                     /gene="flgG"
                     /locus_tag="PLW13_01795"
     CDS             complement(19232..20017)
                     /gene="flgG"
                     /locus_tag="PLW13_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HQY69129.1"
                     /translation="MMNALWVSKTGLSAQDTQLGTISNNLANVSTVGFKRDRVTFEDL
                     LYQIRRQPGAQSSQDNQLPSGLQLGTGVRIVSTQKEFTEGSLQVTGNSLDVAINGRGF
                     LEVLQPDGTTAYTRNGQLHLSRDGEIVNADGLVIQPAITVPEGAQSLTIGKDGTVSVV
                     LPGEAEAQQIGSLTLTDFINSAGLQAIGGNLFLETTASGSPTNGTPGLDGLGTIEQGN
                     LENSNVEVVEELVNMITTQRAYEMNAKVISTSDQMLGYISQNL"
     gene            complement(20050..20793)
                     /gene="flgF"
                     /locus_tag="PLW13_01800"
     CDS             complement(20050..20793)
                     /gene="flgF"
                     /locus_tag="PLW13_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HQY69130.1"
                     /translation="MDRALYTSMTGAKHNTLAQTVHANNLANASTDGFRRDYVAARSM
                     GIWYGDGFPTRVHALTERPATDFASGTLRETGRELDVAVRGAGWIAVQAPDGTEAYTR
                     DGNLQITPFGQLLTGSGLPVLGSNGPIAIPDASKVEIGQDGTITVRAMGQGPEALASV
                     ERIRLVNPPEQDLLKREDGLVHLRDGARAPVDARVRVVSGFLEGSNVNAVEALTEMLS
                     LARQYEMQVKLMKSVDENTEAAARLLQVS"
     gene            complement(20861..22243)
                     /gene="flgE"
                     /locus_tag="PLW13_01805"
     CDS             complement(20861..22243)
                     /gene="flgE"
                     /locus_tag="PLW13_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HQY69131.1"
                     /translation="MSFNTGLSGLRAATSDLNVTGNNIANASTTGFKQSRAEFGDVYA
                     ASLTGTSTIGSGVMLQRTAQQFTQGNIALTQRDLDVAINGRGFFILNNGGERTYTRAG
                     VFGVDKDGYVVSNTGGRLVGYAANSAGQVDLGNEVDLRIEAFKLQPSKTSEVRLGFNL
                     DAAAAAVDGALYPEFNPADQNTFNFASSVTLYDSEGIPHVGTMYFRKDQPDPAIETGV
                     NNDWFMWLAIDGELVNDGNPANPAGAPLDPNGDNSAFLVRFNEDGTLDPATNQFVIDD
                     WVPALQIQSVFDSNAEPLGPLGGAATPTFNGVVDADTSDFLLDISGSTQFGDDFNIDS
                     VSQDGFGPGQLVGTDISENGILFARYTNGQSQILGQIILADFRNPQGLTALGNTQWAE
                     SFDSGQPTRGVATAGVFGSLQAGALEDSNVDISEELVNLIVAQRNFQANAKTIETNNT
                     ITQTVINIRS"
     gene            complement(22301..22981)
                     /locus_tag="PLW13_01810"
     CDS             complement(22301..22981)
                     /locus_tag="PLW13_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HQY69132.1"
                     /translation="MSEINGFKVSGSAYEALSFKKEPAKAENSSLGQADFLTLLITQL
                     ENQSPLTPQDNTAFVAQLAQFSTLQGIEDLNSNFVSMNGSLKSSQAIQASALVGHTVL
                     VEASTARLASGGEIMGTITIDEPVDDTLLNIYNPAGVLVRQELLGRQDAGDITFGWDG
                     RDAAGTSLAPGIYRFEALGLREDETVGLATALGANVNSVTIGANGEMTLNVDGIGPLG
                     ISAVKEIL"
     gene            complement(23018..23449)
                     /gene="flgC"
                     /locus_tag="PLW13_01815"
     CDS             complement(23018..23449)
                     /gene="flgC"
                     /locus_tag="PLW13_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HQY69133.1"
                     /translation="MSINAIFDVAGSAMSAQSIRLNTVASNLANAQTASSSIGQTFRA
                     RHPVFAPLYQDQLGSGGTAQGDAQAGVQVLGIVESDAPLQPRYEPHHPLADEQGYVYY
                     PNVNVVEEMADMISASRSFQINVQVVEAAKTLAQRVLALGQ"
     gene            complement(23449..23847)
                     /gene="flgB"
                     /locus_tag="PLW13_01820"
     CDS             complement(23449..23847)
                     /gene="flgB"
                     /locus_tag="PLW13_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HQY69134.1"
                     /translation="MAINFNAALGVHEQALLVRERRAGVLAANLANADTPHFQARDLD
                     FQALIRQAAGEQDGSRLRATQALHIGAGAPAIAGDLLYRTPMQPTLDGNTVDADIEMA
                     QFARNSLDFQASLTFLQGKFRGLRSALKGE"
     gene            24123..24857
                     /gene="flgA"
                     /locus_tag="PLW13_01825"
     CDS             24123..24857
                     /gene="flgA"
                     /locus_tag="PLW13_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HQY69135.1"
                     /translation="MTMSARLHRLLYALACVLAGLYTLAARAEPATVDARAIAALVEH
                     SVLAQLRDAPLARGAARVEVAAGALDTRLTMAACGQPIRVAADLSRQQARVNARVSCS
                     APSPWNLYVPVEIRVYRPVLVATRELRRGEPIGAADVALEERNVLAAAGAPPLTQAAD
                     VVGLNARRTIAPGTLLSAGLVELPVLVRRGDRLGVSARSGGITVQINGEALGTARLGE
                     RVRVRNLQSGRVIDAVVTGSGSAEAI"
     gene            24972..25280
                     /gene="flgM"
                     /locus_tag="PLW13_01830"
     CDS             24972..25280
                     /gene="flgM"
                     /locus_tag="PLW13_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HQY69136.1"
                     /translation="MVSDIKGFDSRQASGARTGETGAVRSDSRAGAGRQAGAAARDDT
                     VELSGVADLIATAARKLAAEPAVDQARVQEIRKAITNGSYTVDPERIARKLIDADSQY
                     "
     gene            25312..25779
                     /locus_tag="PLW13_01835"
     CDS             25312..25779
                     /locus_tag="PLW13_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HQY69137.1"
                     /translation="MQNLTDDAGVAHAIVAADLAAVRTLRELLLEERAALTRREPEAL
                     NAVVQRKLACLGELQQSEDARKRLLARHRGSDWAALLGTLDPALAAPWEALRANLQQV
                     AELNRVNERIVARTRHSTERLLSMLRGQLDPVGVYDRSGQTRAYGDVRPIASA"
     gene            complement(25746..26405)
                     /locus_tag="PLW13_01840"
     CDS             complement(25746..26405)
                     /locus_tag="PLW13_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HQY69138.1"
                     /translation="MANAEDRRRCGCFLQFAKAPRAGAVKTRLHGQLSPAGAAEVARE
                     LTRRVAAALRAVPAGWEATLCVDDTTDAFLRELAAREGRTLRAQGGGDLGARMWRAVD
                     AALAEYRAVIVVGSDCLGYDAAYLGDAAVALEHGFDAVLGPALDGGYVLAGFTRLPSA
                     VFAGVPWGTDVVLARQRERFAALGLRWHELPGRADIDRPQDLWRLGDAAQALAIGRTS
                     P"
     gene            complement(26392..27072)
                     /locus_tag="PLW13_01845"
     CDS             complement(26392..27072)
                     /locus_tag="PLW13_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HQY69139.1"
                     /translation="MRLSIVIPALEEESALPLLLGDLQPLRARGAEIIVADGGSNDGT
                     VAAAGALADIVLAAPRGRALQLNAGAARARGELLLFLHADSRVPPPCVAAIAALAGAA
                     PHWGFFRVRLRGVSRWLGVVARCMAWRSRLSGIATGDQGLFVPRALFTAVGGYPAQPL
                     MEDIEICRRLRRHGAPLAHPAFIESSGRRWDRDGALSTILLMWRLRLSYFLGADPADL
                     HETYHGQR"
     gene            complement(27075..28034)
                     /gene="arsS"
                     /locus_tag="PLW13_01850"
     CDS             complement(27075..28034)
                     /gene="arsS"
                     /locus_tag="PLW13_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339360.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HQY69140.1"
                     /translation="MQDTRPLLLESDFPAMRRLGVDTLQANLGYRCNQSCAHCHVNAG
                     PSRTEMMDRDTADLLLEVAARHGVTTLDLTGGAPELNPHFRHLVTRARALGLRVIDRC
                     NLSVLEEPGQEDLAQFLADHGVMITASLPCYLESNVDAQRGRGVHARSIAALRRLNAL
                     GYGAGGALELNLVYNPLGPTLPPPQQALELDYKRELRVRYGIGFDRLLTLANLPVARF
                     GAVLLAQGQFDDYLALLKRNFVHANLGQVMCRRLLSVDWRGFVYDCDFNQMLDLPLLA
                     TDRRLHLRDLLERLPLDAPIVTGAHCYGCTAGQGSSCSGALAA"
     gene            28200..28589
                     /locus_tag="PLW13_01855"
     CDS             28200..28589
                     /locus_tag="PLW13_01855"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY69141.1"
                     /translation="MSPTEQLPPGAERRTETRIEVELPARIIARDGRVYAAAIRNISC
                     GGVLVHLAAAHAAALLPNTAREQPRLPVGVRLEFQLPAAPHDAPVAIDCGIAHLRRLA
                     ADQGAMGLNFRHFHAGSEHALARFCAM"
     gene            28671..28745
                     /locus_tag="PLW13_01860"
     tRNA            28671..28745
                     /locus_tag="PLW13_01860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28704..28706,aa:Arg,seq:cct)
     gene            28918..29823
                     /locus_tag="PLW13_01865"
     CDS             28918..29823
                     /locus_tag="PLW13_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69142.1"
                     /translation="MNEAVKDGTQNVLDGKPVIYYDGYWIRYYAPPENTLPQKKALID
                     QMTKRAFHHTEEGINTPGRKLDRARAAWESETDPARKRVNAAMLAGALFNRATDLFTT
                     IVELGEMGVEISMDNELMKQCGECFKEALDLGKYVKHYSGEEGIDELWGEPFKAFTLP
                     IATVYESRYMKVARAMRDIDGLAEKMEQVFGPLAGFAGIAERIRELAAAAKLEAETMK
                     SDPVIFRVWPRFVAACEAVLDFQPALPAPVSDRERREADEALRILCEGKRVIEYLAGA
                     RVPMPKTTAAYHRRCDAFRESIART"
     gene            29820..31235
                     /locus_tag="PLW13_01870"
     CDS             29820..31235
                     /locus_tag="PLW13_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69143.1"
                     /translation="MKQLGVLDSAFINLELPGTPQHIGGLGIYDPSTAPGGFVRFKSV
                     IAGFERRLRRTPQFRARLQQVPGGIDRPYWIIDEDFDLEFHVRHIALPAPGDWRQLCI
                     QVARLHARALDFSRPLWEAWIIEGLDNLPGLPRGSFAIYGKLHHALFDGAGGQVFVDA
                     LHDLTPVPAEAAPACAQDRLYADEALGEVQMLGRAAMNQLVNGIDMAQGALRAGGEIA
                     RMLLGHGEDGGKSLLASAPRTRFNEPVGPRRSFEAASFALADFRALRNATGARLNALA
                     LTVIGGALRRYLGARAELPERALNAAIPLDLRRRRADSGDTNQVGTVYLSLHTDLADP
                     LQRLEAVRREADRARERGARNPLVDTMKLAGLLPPAVVRAASEFYAAHGLTRRMPLVT
                     STVVANAAGPEIPLYCAGARLVRYHGLGPLSPGMGIYHTVFSCNDTLTISVLADREHM
                     PDPAFYRECLEESFAELRDAL"
     gene            complement(31299..32147)
                     /locus_tag="PLW13_01875"
     CDS             complement(31299..32147)
                     /locus_tag="PLW13_01875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQY69144.1"
                     /translation="MSALTGPLIDPLRLHALLAGAHPPLVADCRFALADPAAGEAAYR
                     AAHIPGAVYLHLERDLSAPVAATGGRHPLPSPGAFGRTMRRIGLDAGRLLVAYDDSRG
                     AFAARLWWLARYFGHERVCVLDGGVTAWTRAGLALDTAIPAPAPGDFEPRPRAAMRID
                     YEELAPRVAAGTLALVDSREPRRYAGLEEPIDPVAGHVPGALNRPWAGATREADGCFL
                     DAAAQAERWHGVDARELVVYCGSGVTACANLLSLELAGIRGARLYPGSWSDWCSRPGA
                     PVARGT"
     gene            complement(32203..32988)
                     /locus_tag="PLW13_01880"
     CDS             complement(32203..32988)
                     /locus_tag="PLW13_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQY69145.1"
                     /translation="MPRSNRFREVLQPAPGALLLALAWFCLAAGVAVADPLAEAMARQ
                     QALVAAWPVGERVAGFKSAFNSPQMQQRFGLDRAALAVLPASAERCAGQAAGCTVSRA
                     GYERMVLEIEVALRLGAAITQPLADEAALLARIDGVMPAVELPELALEAKVRPPNGID
                     YIASNIGVRGYLLGAVRDPALLQGAAPALMLTRGATVLAQGHADGALTAALELVNAAV
                     AAGYELRPGQVLLSGAVGAMVPAEPGDYVADCGALGTLRFTVR"
     gene            33038..34363
                     /gene="rimO"
                     /locus_tag="PLW13_01885"
     CDS             33038..34363
                     /gene="rimO"
                     /locus_tag="PLW13_01885"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQY69146.1"
                     /translation="MNQPKVGFVSLGCPKALVDSERILTQLRLDGYSIVPSYQDADVV
                     VVNTCGFIDSAKQESLDAIGEALSENGRVIVTGCMGAQAEQIMKVHPQVLSVSGPHAY
                     EAVVGAVHQHVPAPDRQHDPFVDLVPPQGVKLTPRHYAYLKISEGCNHRCSFCIIPQL
                     RGDLVSRPIGEVMDEAERLVKAGVRELLVISQDTSAYGVDLRYRTGFWQGRPLKTQMQ
                     ALCSALGELGVWVRLHYVYPYPHVDNIIPLMAEGRILPYLDIPFQHASPRILKLMKRP
                     GNAEGVLERIHAWRETCPDLTLRSTFIVGFPGETEAEFEELLDFLDEAELDRVGCFKY
                     SPVDGAPANALPDPVPEEVKEERWQRLMELQAEISAERLQQRIGREIDVIIDRVEGDS
                     AIARSSADAPEIDGVVHIENVEGLVSGEIIRVRVEGADDYDLWAVPVEA"
     gene            34360..35133
                     /locus_tag="PLW13_01890"
     CDS             34360..35133
                     /locus_tag="PLW13_01890"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008700.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQY69147.1"
                     /translation="MNLLLLAAAEIAAPGLAEVALSRYPARAGWWPPQPGRRLLVGLR
                     DGLLGEGVVEHLGQRHARIRFTLDRAPPPALPLTLLLALPRPKMLRRVLRSAAEIGIK
                     HIWLINSSRVEKSYWQSPLLAPPMLDAYLAAGLEQACDTVLPRVELRQRLRPFVEDEL
                     PAIAPGSLRLVAHPAAGAPLGPAGAGHVTLAMGPEGGFTDFEIALLGAAGFAPCTLGP
                     RVLRVETALPVLAANLFPAGFSNAAADDATCRPPATAHP"
     gene            complement(35075..35929)
                     /locus_tag="PLW13_01895"
     CDS             complement(35075..35929)
                     /locus_tag="PLW13_01895"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY69148.1"
                     /translation="MSEYPSNSLHQPGPFGRLLRFWRATFGLSQEELALETGVSARHV
                     SFLETGRSQPGRSMVLALAGVFRLAPQDLNTLLVAAGFAPDAPGVDMQAPEMRWLHKS
                     LELNMRALDPWAATVQDKYGNLHLVNRGWLAMLRALLAPEFLAPPINSYHMFFAENGL
                     RARLVDWEDVACRLLMTLQQEVLLSGDADAARLLGQLLAYPAIPEQWARRGAEIAFQN
                     SFRLRLAMPDGDVRSYLYTVNTVGATPYVGEPRLLLSLLTPEDLRQEFTGAQLLADDT
                     LRHPLLHY"
     gene            36088..36867
                     /locus_tag="PLW13_01900"
     CDS             36088..36867
                     /locus_tag="PLW13_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69149.1"
                     /translation="MTTTASPRPTPPRLAYALTEPGRFVIEINRLLLAHPFLRRARGG
                     DGHPVMVLPGFLGADGSTAALRYYIANWGYDTHGWAMGRNMGLRAEDPEFENRLAERL
                     GTLVRKSGRKVSLVGWSLGGVLARELARACPDHVRQVITLGSPIGGDPKATTIWRVYE
                     LATETELHSEELRQRVAQIVQPVPGVPCTAIYSRSDGIVSHRIARERETPLTDNIRVV
                     ASHVGLGFSATVLHAIADRLAQDENDWRPFRRTCWRRIFYA"
     gene            36908..>37398
                     /gene="xseA"
                     /locus_tag="PLW13_01905"
     CDS             36908..>37398
                     /gene="xseA"
                     /locus_tag="PLW13_01905"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQY69150.1"
                     /translation="MKREGNARQVLSVSDLNRHSRELLEAAFPSVWVEGEISNFSAPG
                     SGHWYFTLKDSDAQVRCAMFRNRNLRARLRPAHGDRVLVRGSVSLYAARGDYQLIVEE
                     LEAAGAGALQRALELLRQRLAAEGLFAPERKRALPTFPAHLGVITSATGAALHDILTV
                     LRRR"
BASE COUNT         5950 a        13356 c        12513 g         5579 t
ORIGIN      
        1 cgggcacgat cgcattgctc aagggcctcg gcttcaaccg cgtcagcatg ggcatccagg
       61 acttcgaccc ggccgtgcag cgcgccgtca accgcatcca gcccttcgac atggtcgaga
      121 acctggtcga cgcggtgcgc gagcaccgtt tcaagtcgct gagcttcgac ctgatctacg
      181 gcctgccgct gcagagcccg gcctcgatgg agcagacact ggcgaaagtg ctggcgctcg
      241 atccggaccg agtgtcctgc tacagctatg cgcacctgcc cgaaatcttc agcagccagc
      301 gcagcatcga tcgcatggcg ttgccgagcg ccgacgagaa gctcgaggtg ctgcacacca
      361 tcatcgacac gctgaccgac gccggctatc tctacgtggg gatggatcac ttcgtcaagc
      421 cgcaggacga gctcgcgcag gccctgcaca acggtacgct gcaacgaaac ttccagggct
      481 actcgacgca gctggcgccc gaactgctcg gtatcggcgt gtcgtcgatc agcagcaccg
      541 ccgacctgta ctgccagaac gtgaagaccg ttgacgaata ctatgccctg ctcgagcagg
      601 acaagctgcc ggtgcagcgc gggctgcggc tcacccccga ggaccagctg cgccgcgacg
      661 tgatcatgca gctgatctgc cgcatgggta tcgacaccgg ggagaccgga gaacggcacg
      721 gcatcgactt cggcagccac ttcgcgcgcg agatgcgtat cctcgacgac atggagcgcg
      781 aaggcctgct ggcgagcgag ggcggacaga tccgcgtgac cgcggcgggc cgtccgctgg
      841 tgcgcaacat ctgcatggtc ttcgacgcct acctggaccc ctcgcgccag cgcttttcgc
      901 gcacgatctg agcctgcctt ccgcggcgga tgggtcgccc gggcagcgga cacggctgcc
      961 tgttcatacg catgccctct actttatact cggccgatac tgcggaatcg gcaccagggg
     1021 gctgcgccgt gacggaactg ctgaaatccc atgacgtcaa tgcaaccggg catcggtgcc
     1081 cccacgacta ccagataaac tgcggcaact gccggctcaa cagcatctgc ctgccgatcg
     1141 cgctcgacat ccacgaggtc gatcagctcg aggaaatcat ccagcgcagc cggccgctgc
     1201 agaagggcga gcacctgtac cgccaggacc agccattcgg ctccgtgttc gcggtgcgct
     1261 cgggcagcgt gaaggcctac gccatcaccg acaacggcca ggagcaggtc accgggttct
     1321 acttccccgg cgaagtcctc ggcatggacg gcatcgcgaa gaacaagtac gccagctcgg
     1381 cgcgcgcgat ggagaccgcc gcggtctgcg agataccctt cgacaagctc ggcgagctca
     1441 gccagcgcct gcccagcctg cagcgtcact tcttccagct gatgagccag gagatcgccg
     1501 aggaccagca gttgctcacc ctgctcagca agaataccgc cgaccagcgc gtcgccacgc
     1561 tgatgctcag catctccgcg cgcaacaagc gacgcaagct ctccgcggcc agcttccgcc
     1621 tgccgatgtc gcgcaccgac atcggcaact acctcggcct caccgtggag acggtcagcc
     1681 gcgtcctcag ccggttccag aagctgggcg tgctctcggt cgacaacaag gagatcaaca
     1741 tcatcgacgc cgagctgttg cgctccgtgg ccgacggtag cgaggcaacc tgattttcgc
     1801 gcttgacgca ggtcaagtga aacctgtccc tcgcctgtta tattccgcgc cgatcgcagc
     1861 ggtacctgcg gtctccgttt tctcgattcc ataacacacc aggagcacgg gcatcacgat
     1921 gatcaccaac gcaatcggca ttctgttcag cccccacagc acgtggcagc ggctcgccgc
     1981 ggtcgccgac cgggcgagca acctgttacc catcctgata ctcgccgtcc tgcccgccgt
     2041 tggctggtac tacggcacca cgcacatggg atggacggtg ggctccggcg aagtgacgaa
     2101 gttcgcgcgc tcgggcggca ttgccctggg cgcagcgctc tacgtgggcc tggtggtatg
     2161 ggtctgcatc atcgggtggt cgatccactg gatggcgacc agctacggcg cgcaatcgac
     2221 gctgatgcgc ggcatcaccg tggccggttt caccgccgtg ccgatgctga tcgccggagt
     2281 ggtcggcttc aaccccgtgc cggccatcgt gatggtggtc ggcctcggct cggtggcgtg
     2341 gagcctgtac ctgctctaca tcggcattcc cgcggtgatg aaggtgccgc aggaccgcgg
     2401 cttcctgttc gcgagcgccg tggtggcggt ctgcctggtg atcctgatcg cgatgatggg
     2461 tgccggcgtg atcctgtggg acatgttcca gccggaattc tccaactgat ggctcgcgcc
     2521 gggtccgggt gcccgcgaga cgctcaggcc ccgagcgcag cctcgacgtc cagcccgctc
     2581 tcgcgcatcc gcgccagctt gtagcgcagg gtgcgcgggc tgatccccag acgctcggcc
     2641 gccgtcgcct tgctgccgcg cgtgtccttc agcatgttca cgatgagctc gaactcgcgc
     2701 aggcgcaggt cgccgttcag cccgctggcc tggtcatcgg catcctccgg cggtgcgtac
     2761 tgcgccgccg gagcgggctc gccgaggctc gccgatgacg ccaccggctc gaggcgcagg
     2821 tgtgccgccg acaccacgac gccgtcctgc aggatcagcg cccgctgcat cgcattgtcc
     2881 agttcgcgca cgttgccggg ccacggatgc gcgagcatgg cgcgcaccgc gcccgcgtcg
     2941 aactgcaccg gcacgtggtg catcttcacc gcgtgccggc tcaccagccg ttgggcgagc
     3001 ggcaggatgt cctcgccgcg ctcgcgcagc ggcaggcagc gcagcgggaa tacgctgagc
     3061 cggtagtaga gatcctcgcg aaagcgcccc gtggcaaccg cggcgcgcag ttcgcggttc
     3121 gtggtggcga tgaccctgac gtccaccgcc agcggccggc gtccacccag gcgctccacc
     3181 tcgcgctcct gcagcacgcg cagcagcttg gcctgcagcg aggcatccat ctcggaaatc
     3241 tcgtcgagca gcagcgtgcc accgtcggcc tgctcgaact tgcccggcat ggcggtgtag
     3301 gcgccggtga aagcgccctt ctcgtggccg aacagcatcg cctccagcat gttttcggga
     3361 atcgcggcgc agttgatcgc cacgaaggcg tgttcgctac ggcccgaatg gcgatggatg
     3421 tagcgcgcca gcacttcctt gccggtgccg ctctcgccgc acagcagcac cgtggagtcg
     3481 gtcgcggcga cccggcgcgc gagttgcagc aactcgaccg attcgcgcgc caccgccacc
     3541 ggatcctcgt cgcctgcggc cgccccggcg cccaggcgcg cgatcacctc gagcagctgc
     3601 gccggctgga acggtttcac caggtagtcg gaggccccgt cgcgcatcgc ctgcaccgag
     3661 cgctcgatgc tgccgaaggc cgtgatcagc accaccggca cctgcggatg cagcgcgcgg
     3721 atgcgtcgca gcagttcgtg gccgtccatg ccgctcatgt tgacatcgga gaccacgaca
     3781 tcgaccgcat tctgcgcgag cagcgcgagc gcctcgggcg cgccgggtgc cgccatgacg
     3841 cgacagcccg ccagctccag ggtgtcggcg agcgcctcgc gcagctccgc atcgtcctcg
     3901 acgatcagcg cgcgcgtgcc gcgcacgccg tgctcacgca cgagcgcgac ctgcggcatc
     3961 gacgccgcgc tcgtgcagcg gcagcaacac gctggcacag gtaccctgcc ccggcaccga
     4021 gcggatctcg agctccccgc cgtgcgcgcg cgccaccgcc cgcaccaccg cgagcccgag
     4081 gcccgtgccc tggggtttgg tggtgaagaa cccgtccccg agctgcgcct gcacttcggg
     4141 gctcatcccg ggtccgttat cgcacacgct gatgcacacg cggccctcgc gcagtgccgc
     4201 ctgcaccgcg atcagtgcgc cctgccccgc agcctgcaac gcattggtca ccaggttcag
     4261 caaggccccc gagagcactt cccggttgca cagcagctgc aacgtgcgat cacaatcgat
     4321 gcgctgctcg cagcgcgcct gcgcactggc cagcggcgct tccatggcag cggcaatatc
     4381 ctcgaacagc tccccgaggc tgctcacgtt ctccagcgcc acatcgccgc gcacgaagat
     4441 gagcatgtcc cgcacctggc gctcgaggtg ggtcaggcgc gagaggatct tcgccgagaa
     4501 cttgcgggtc tgctccggcg tcagttgcgc gtcgcgcaga ttcgaggcgt agagcatcgc
     4561 ggcggccagc ggggtgcgca gctggtgcgc cagcgccgcc atcatgcgcc ccatcgcgtt
     4621 gaggcgctgg tggcggctca ggcggtcctg caactcgcgc gtctcggtct ggtcggtgag
     4681 cagcacgacc tgccccttgt cgcgctcgag cgagcgcgtg gccacgctga cgcggcgtcc
     4741 gctggcgagc gatatctcgt gcccgtcgtc gtgacgcggc gcgaagcagc gccggatcac
     4801 ctcgacccag cgctccccga gcacgccgcc ctgcagcatc tcgcgcgcgg cggggttgca
     4861 gtcgcagacg atgccgtggc ggtcgagcac gaccacgccg ccgggcagca ggtcgagcag
     4921 gctgcgcagg cgctccgaga cgcgttcctt gccttccagt tcacgcagcc gcgcgcgatc
     4981 gacctgctcg agctccgagg agagttcctc gacgcggcgc tcgagcaggc ggtaggagtc
     5041 gctgagctcc gcggaaacct ggttgaacag ggcgaaggcc gcctggattt cggcgctgtc
     5101 gcggaccgac ccggggacga tatcgcgcgc ctgcgcgagc gatgcgctca tcgagccgca
     5161 cccgcgcgga ccctgcccgg aacccgacgc aactgctcag gcatctgacc accacccgga
     5221 aaattgacat tccggcaagg tctagcaatt cacatgcccg atttattttg tcgctttgtt
     5281 tcagatagtt ggaacaggtt cccggagatc aacgcaaatg cgtcaagctt ccgccgctgc
     5341 ggccgcgggg ttcagcaggt cgtacttgcg catcttctcg accagggtgg tgcgccgcag
     5401 gcgcagccgg tcggcggccc gcgcgaccac gccgctgcaa tcgtccagcg cctgctcgat
     5461 caggctcttc tcgagatcct ggatgtattc cttgaggtcc agcccgttga cgggcagcaa
     5521 tcccgacgcg ttcgcggacg cgggcgccgc ggtctcgagc ggctcggggg ctgcgggcag
     5581 tatcacgtcg tcttcctcgt gcgcctcgag atggcggaat ttcggcggca ggtcgcgcgc
     5641 cccgaccacc gcgtcgggat gcatgatcgc catgcgctcg accaggttgg cgagttcgcg
     5701 cacattgccc gcccacgggt ggcgctgcag ggaggccagc gccgcggcac tgaaacgcac
     5761 gctgccgcgc tgttcgcgct ccagccgcgc ggtgagctcg ttgaccagca ggggcaggtc
     5821 ctcggagcgc tcgcgcaacg gcggcatctc gatcggaaac acgttgattc ggtagtagag
     5881 atcctcgcgg aaactgccct tggcgatcat ttcctccagg ttcttgtggg tcgcgcaaac
     5941 gatgcgcacg tccgcttcca gcgtctggct gccgccgacg cgctcgaagc ggcgctcctg
     6001 cagcacgcgc agcagcttca cctgcatcgc gagcggcatg tccccgatct cgtcgaggaa
     6061 cagcgtgccg ccctgcgcca gttcgaaacg gcccgcgcgg ctgctgacgg caccggtaaa
     6121 tgatcccttc tcatgcccga agagctcgct ttcgagcagt tctcccggga tcgcaccgca
     6181 gttgagcggc acgaagggac cgctgcggcg gcggctgtgg gcatgcaggc tgcgcgccac
     6241 cacctccttg ccggtaccgg attcgccgag caccagcacc gtggcatcgg tatcggcgac
     6301 ctggctcatc atgcggcgca cggcgcgcaa gcctgcgctg gcacccacca gctcgcggaa
     6361 cagcttcagc tcgccattgc cgcgaccgcg ccccagcgcg tactgctcgc ggtagacgcg
     6421 cgcgcggtgc agcgcatcga ccagacgggg gtaggcgaag ggccaaccca gcgtgccgat
     6481 cagcatcgct tcgtgacgcc ccggcgccac gccatctgcg ggcgccgcgg tgatcaccac
     6541 cggcacgccg ggatccagtg cggccagcgc ctccagcgtc gcctcgagat cgaagccctc
     6601 gccgtcggcg gccagcaaca ccacctgcgg cggctcggcg agcgccgctg gcagcgccgc
     6661 cagtgcggtg tgcgattcgt cgaggaagcc gagaatggcg gaggcggtcg cacggcgcgc
     6721 ggcgtcgtga tccagcagca ggatgtgggt acggttcgac atgcgctcgt ctgatccctg
     6781 gcgagcgccg gatggcgctg ctcacggatc gattatagat ggcgaatacc gcaaaccgac
     6841 agatttacgg gcacgaaccc cggacattct gtcaaaaaga tgacaccgcg cccgtcaaga
     6901 cgtcgcagcc tgcccgcgaa tgccctccca gccactgcgg atggtgccca gcaatgcccc
     6961 cacctcgtcg agcggctcga cgctctgccc gcggtgcacg tccatgaggc gcagcgtcat
     7021 gtagtcgtag agccgatcca ggttggcggc gatctcgccg ccggcctcct tgtcgaggca
     7081 gccctgcaac ccgctgatga tcccgagcgc cttgcccagg gcaacgccga cggcctcggc
     7141 atcaccgcag gccagcgcac ccttggcctg cgcgatgcgg tcgatggccc catcgagcag
     7201 cagctggatc aatcgatgcg ggtccgcatc caccacgccg gtctgtgcat tgatcttctg
     7261 atactgcgcc agggcgctct tgctgtacac gtactgatac ctcgtcgcct gtccgggcgg
     7321 acatccggct gtctgaactg cgcagaagtg cccgcagcca acccggccga tgcctgtcga
     7381 ccgggcgccg cacgcctcga gcggcaatga tgacacgcgc acgacccgct ccggccggag
     7441 aaggcgaaaa attttcttaa agttcctgca acccgcgccg ataccggaag cgaggccgtt
     7501 gtcgcggcca tcgagtgaaa cggaactcaa ccagcacagc ccctgccggg gcagccttcg
     7561 gagacgcacc atgccgcaga tcatcaacac gaacatcccg tcgctgaacg cgcagcgcaa
     7621 cctcgacaag agcgggcagt cgctggcgac ctcgctgcaa cggctgtcct cgggcctgcg
     7681 catcaacagc gccagggacg atgccgcggg gctggccatc agcaaccgct tcacctccca
     7741 gatccgcggc ctgaaccagg ccgtgcgcaa cgccaacgac ggcatctcgc tgtcgcagac
     7801 cgccgagggc gcgctggccg agaccggcga gttgctccag cgcatgcgcg aactgtcgat
     7861 ccagtcggcc aacggcagcc agtccggcac cgagcgcgcc gcgctgcagg ccgaggtctc
     7921 gcagctgcag caggagatca accgcatcgc cgagaccacc accttcggcg gccgcaagct
     7981 gctcgacggc accttcggca ccgaggcctt ccaggtcggc tccaacgcca acgagaccat
     8041 ctcgatcagc atcgccaact cgcgcgccac cagcatcggc gccaacacca tcgccagcag
     8101 cggcgacatc aatgcggccg ttgcagccaa cgggaccgtg caaccgaaca acatcgacgc
     8161 acagagcctg acgctcgcgg gcaacctcgg cagcagcacg atctcgatcg ccgccggcgc
     8221 caccggcgac cagatcgcca gccaggtcga ggcggtcagc gccaacaccg gcgtgagcct
     8281 gacggcgcgc accaccgcga cctttgccgt cggcgcgacc ggcacgctgt ctttcaacat
     8341 cggctcggtc tcgggcagca cgagctacag cgccagcgtc agcgtgctgg tcacggatgt
     8401 ctcggacctg tcatcgctgg ccgacgcgat caacgccagc tccgccgcca gcggcgtcac
     8461 ggcgaccgcc aacggcgcca ccctgagcct cgtcaacgag gaaggccgcg acatcttcgt
     8521 cggcgacttc gccaacagca ccgccggcaa ccagactgcc acgctgacgg gtgcatcggg
     8581 cgcggcggtc accctgaccg agggctcgac cgactccagc acggtaggcg ccaccctgag
     8641 cttcaccagc taccaggcct tctcggcgac caccaccagc gccaccggcg gcatcttcac
     8701 cgccctcacc aactccagcg cgctcagcag cgtggccagc atcgacatcg gcacccagca
     8761 gggcgcgcag gatgcgctgg cggtcgtgga cgcggcgctg cagttcatcg acgccacgcg
     8821 cggcgatctc ggcgccgtgc agaaccgcct gctcagcacg atctcgaacc tgcagaacgt
     8881 caccgagaac gtgaccgccg cgcgcagccg catccaggac gcggatttcg ccgccgaaac
     8941 ggcgaacctg acccgcgcct cgatactgca gcaggccggc atctcggtac tggcacaggc
     9001 caacgcgttg ccgcagcaga cgctggccct gctccagtaa caggggcatg acttcggggc
     9061 atgaagtcga cggggcagag cgccgctctg ccccgctgcc ctcgccctga agcgagcgcc
     9121 aggactcgat catggacacg tcacgcatcg catacggcca ggcccagccg atcgcaggca
     9181 ggggcaaggc cgtgtccgcc gatgcacaac cgaatccggc gggtgcgggc agcgcgggca
     9241 ccgcaactcc cgcgcccgcc gccactcccg cgctgtccgc cgccgatgcc gcgcaggcgc
     9301 cgctggagga cctgcagcaa ctgctcgagc aggcaagccg gcacatccat cccgagcaga
     9361 ggtctctttc cttcgagatc agggacgaac tgggccgcgc ggtggtgtcg gtctatgacg
     9421 ccgagaccga ggaactgatc cgccagatcc cttccgaaac cgcggtgcgc atcgccaccg
     9481 cgatgcgcga gatgacgcag cagggcagcg gcgcgctgcc gccatcaggc ctgctcctgg
     9541 acgaacaggc ctgatgccag gcaacaaaaa ctcaaggaca cgggaagcac catgccgcag
     9601 atcatcaaca cgaacatccc gtcgctgaac gcgcagcgca acctcgacaa gagcgggcag
     9661 tcgctggcga cctcgctgca acggctgtcc tcgggcctgc gcatcaacag cgccagggac
     9721 gatgccgcgg ggctggccat cagcaaccgc ttcacctccc agatccgcgg cctgaaccag
     9781 gccgtgcgca acgccaacga cggcatctcg ctgtcgcaga ccgccgaggg cgcgctggcc
     9841 gagaccggcg agttgctcca gcgcatgcgc gaactgtcga tccagtcggc caacggcagc
     9901 cagtccggca ccgagcgcgc cgcgctgcag gccgaggtct cgcagctgca gcaggagatc
     9961 aaccgcatcg ccgagaccac caccttcggc ggccgcaagc tgctcgacgg caccttcggc
    10021 accgaggcct tccaggtcgg ctccaacgcc aacgagacca tctcgatcag catcgccaac
    10081 tcgcgcgcca ccagcatcgg cgccaacacc atcgccagca gcggcgacat caatgcggcc
    10141 gttgcagcca acgggaccgt gcaaccgaac aacatcgacg cacagagcct gacgctcgcg
    10201 ggcaacctcg gcagcagcac gatctcgatc gccgccggcg ccaccggcga ccagatcgcc
    10261 agccaggtcg aggcggtcag cgccaacacc ggcgtgagcc tgacggcgcg caccaccgcg
    10321 acctttgccg tcggcgcgac cggcacgctg tctttcaaca tcggctcggt ctcgggcagc
    10381 acgagctaca gcgccagcgt cagcgtgctg gtcacggatg tctcggacct gtcatcgctg
    10441 gccgacgcga tcaacgccag ctccgccgcc agcggcgtca cggcgaccgc caacggcgcc
    10501 accctgagcc tcgtcaacga ggaaggccgc gacatcttcg tcggcgactt cgccaacagc
    10561 accgccggca accagactgc cacgctgacg ggtgcatcgg gcgcggcggt caccctgacc
    10621 gagggctcga ccgactccag cacggtaggc gccaccctga gcttcaccag ctaccaggcc
    10681 ttctcggcga ccaccaccag cgccaccggc ggcatcttca ccgccctcac caactccagc
    10741 gcgctcagca gcgtggccag catcgacatc ggcacccagc agggcgcgca ggatgcgctg
    10801 gcggtcgtgg acgcggcgct gcagttcatc gacgccacgc gcggcgatct cggcgccgtg
    10861 cagaaccgcc tgctcagcac gatctcgaac ctgcagaacg tcaccgagaa cgtgaccgcc
    10921 gcgcgcagcc gcatccagga cgcggatttc gccgccgaaa cggcgaacct gacccgcgcc
    10981 tcgatactgc agcaggccgg catctcggta ctggcacagg ccaacgcgtt gccgcagcag
    11041 acgctggcat tgctgcagta gcgcactgat cactccggcg tgttgccccg cgaaccggcc
    11101 tggggtgtaa tgaccggcag ttcgccgccg ggtccggacg cggcgcggcg ggcgatccac
    11161 aggagaaacg gcaggcatgg ccagtatcgg atcattgggc gtgggttcgg gactcgatct
    11221 cgagaccctg gtcacgaaat tgatcagcgc cgaaggcacg ccacgcagca acagtctcgc
    11281 cagcaaggaa gccagcatcc aggccgatat ctcggccttc ggctcgctga agagcgcgct
    11341 cgacaagttc cgctccagcg tggccacgct cggtgacgca aatgcccttc aggcccgcac
    11401 ggtaacgacg ggcaacagcc ggtatttcag cgccaccgcg ggcgagcgcg cggtttccgg
    11461 gcaatacgcg gtgcaggtgc tgaacacggc gcgcgcccag aagctggtga gcactgccga
    11521 cttcgcgagc gcgagcgata ccgtgggcgc aggatcgctg acgatctccg tcggcagcgc
    11581 gtccttccag gtcacgacca cggccagcac caccatcgcc gagctgaaga ccctgatcaa
    11641 cgacgctgcc gacaatgccg gcctcagcgc ctcgctgcta gtggtggcgc gcgatccgca
    11701 aaacgccgct gccggcacgg tggcgcgcct ggcgctggcc gccgatgaaa ccggcagcgc
    11761 caacaccatc gggatcagcg tcagcgacgc cgacgcgaac aacaccgacg cgctggggct
    11821 ctcgcgcttc tatttttcct ccgccgacat cgccaacagc cagctcggcg agcagcaggc
    11881 ggcgctcgac gcgcgcatcg ccgttgacgg gcagacggcg ttcagcaaca gcaacgtgtt
    11941 cagcgacgtg atcgacggcg tgacgatcac ggcgctgaag gatcctgcgg atccgctgaa
    12001 tccggatgtc gaaaccctcg gcatcgccct cgacaacaac ggtgttgctg cccgcatcag
    12061 cgccttcgtc acggcgtata acgaggtggt cagcgcgatc agggacgttt ccgcctacaa
    12121 cgccgaggcg ggaaccgcgg gcgcgctcaa cggggatgcc acggtacgcg gcatctcctc
    12181 acggctgcgc acgatcatcg gcgccgcggg cattggtgac gggacgccgc gatcgctggc
    12241 cgagctgggc atccagaccc agcgcaatgg aacgctgaag ctcgacaccg cgaagctcga
    12301 tgccgcgctg aaggatgatt ttgccggcgt cggcacgctg ttcaccgcgc cagacggcgt
    12361 tgcgacgcga ctcgagtcca tgctcgatgg tttgctcgag cgtggcggca cactggccac
    12421 gcgcaccgat gggctggacc ggcaactcaa ggcgataggc accgagcgcg acaagctgga
    12481 gatccgcctc gagaagctcg aagcccagta tcgcgcgcgt ttttccgccc tcgattcact
    12541 ggtcgcgggg ttgcagagca gcggcgacta cctgctccag cagttgcaga acaccgcgag
    12601 catcatcggc cgcaacccgg gcggcggtaa ctgatgcaac ccgatgcgac cggcgtgcgc
    12661 gcggcgctgc cggcgcgcct gcgcgatgcc tgggaccgca tcgccacgat cgatgccgcg
    12721 ctggccgcgg cgctggcggg cgaaacgccg gccgatgtgg cgcaactcgg cgcgcagcgc
    12781 acccgttgca tcgaagagtt cttcgatgcc ttcccgctcg aggcgcaaac cgccgggctg
    12841 cgcaccgagg cgctgcgaca cctgctcgcg atgaacaatg cgctcgccgc ggcggcgcaa
    12901 cgggaactcg ccaccgcgag cgaggtggcc accgcggcgc gtcatcagcg caaggcgata
    12961 tccgtctacc acgaggtcca gccgaaagcg taggtccgca ttcatgcgga cgattgtccg
    13021 gctgaagccg gacctgcgcc ggcgtgcgga tggcccggac tcagcgcagg aaattgaaca
    13081 gcgacaggtt cgctatcttc gccaggctct gctgcgcggc ctccagcacg aagctctgga
    13141 acgagagccg gctcgcggct tcatcgaaat cgaggtccac caggtccgac agcgcctcgt
    13201 cgttgatcag gtcgagcgcg ctctgcacgg cgcgcgcatc gtccagcagg ttcagccgcg
    13261 gcccgagcga ggcgcgactc gccgtgaccg actgctccga gcggtcgatc ccgttcaggg
    13321 cctcggcgac cactcgctgg cgctcgatcg cgcccgcctc ggtgtcggga atgttgcgca
    13381 gaccgtcggc caggcgactc accgtgagca gcagcgactg cgtcgcgctt gcctccagca
    13441 cgaactcgtc gcccggcgcc ggcgagcccg tgatgcgcac ctcggcacca gcgacagtga
    13501 tggtcaacgg cgcgccatcg acatagggct gcggtggcag cacgacgctg gccgcccccg
    13561 tcgcgcgatc gatacgatcg acctggtagg tgcccggcgc aacgccgaag cgcaccacca
    13621 ggtcgtcggg atagacggca tcgtaggcgg cctggtccac gatgcgcccg atggagatgt
    13681 cgccggtgcc gctgttgagc gccgcgttgc gcgtgacaaa ggtactcgcc gcggccggga
    13741 tatcgacgaa cagctcgcgc ccgctgttgc gcgactgcac ctggagcccg gcgccgatcg
    13801 ccacgctgcg ctcgccatcg tcgccggcat agtcgatgcg tcccgcggcg ctctgcacga
    13861 agggctgcgt gtcgccgctg aaacctgcga agagataatc gccgtaggcg ctgcgcgtat
    13921 tgaaaatgga cagcaactgc tgcccggcct gctcgatgcc ggccacgagc gtggcgcgtt
    13981 cgttcgccga cagcgtggca ttccccgcct gaagcgcgaa ctcgcgcaac tgcagcatca
    14041 cgttttccac ggcctcgagc tgcgcatcct gctgctgcag gtcgctttcg gcgagatcgg
    14101 cattgcgctg gtactgcgcg ctgcgggcct gctcctggcg aatccccagc gccagcgccg
    14161 cggccgccgg gtcgtcggcc ggcgtcagca cgcgcttgcc cgaggagatc tgctcctggg
    14221 cgcgcgcgac ctcggcctgc gcgttcgtga tgtgacgcac gcccgtgttg aatatatgca
    14281 tcgacgagat acgcattggt cctctctcaa ccgagcgcgt cgaacagagc gtccagcacc
    14341 gcgcgcgccg cgctgattac ctgtgccgag gcattgtacg cctgctcgaa gcggatcagg
    14401 ttggcggcct cctcgtccag gttcacgccc gacaggctct cgcgccgctc gcgcgtctgg
    14461 tccagcaccg caaggctcga ctgcatgtcg atgcgcgagc gctgcgtgag gctcccggtc
    14521 gcggccacca ggtcgcggta ggcgccctgg tagctggcgc tgccgttgcc catcgtgtcg
    14581 gccgactgga gcgccgccat cagcagcgcg ttgcggttgt ccgatgtccc gccgccgttg
    14641 tagtcgatgc gaaaggcatc gcccgccgcc ggctcgcccg cgagcgcgac ctggaagctg
    14701 aagtaatcgg gatccgagct gtcgcggctg aacagggtgt tcgccgtccc gggaatgaag
    14761 ccgaggccgg cgaccagcac cgccgaggtg ttcgcatcga gcacgtcaaa ctgcgtcgcg
    14821 ctgctgaagc ggatcagcaa cggcggggag agctgtcccg gcgtggcgaa gatcgcggtg
    14881 ccggtgtcga atgtctcgcc cggcgcaatg gagccggtgc ccaggttgcc ggtcagcgcg
    14941 gctgcacgca cgggagcggc gagcgccagg tcctcggcgc gcgacatcac cgtgcggacg
    15001 aactgcgcgc cacgccgggt cggctcgatg agaaagctgt cgccggcgac gaagttgccc
    15061 gggggcacct cgctcaggtt caggtccagc gtgaagccgt cgaccgtcac cagcgtgtcg
    15121 ccgaggctgg cgccgctcgc caccagcgtg gcgtcctgca accgcaccag ccgccacgat
    15181 gccccgtcgc tgccgatgtc gagccggtaa tcgctggtgg tgagcttcgc ggtatcctcg
    15241 atgaacacgc gcacgccgcc ggtggaggcc ggatcgttgt cgctcgccgg tcgcgcgcgc
    15301 agcaccgcgg cgatctgctc gttgacgtcg ctgaacacca gcccgcccag gtcgccgtcg
    15361 aggtcgacgc ccaaccggtt ctgcgcgttg atgtcgtccg tgagcgccag cgcgacgcgg
    15421 cccagcgcgt tgaacgccgg gtacagcagc tcgtcgcgaa atgccagcag cccgccgacc
    15481 tggccttccg tcgccgcgta gctgaccacc gaggttgcgc cgttgagctc gagggcgagt
    15541 tgcagcttgc cgggatcgaa gcggtccggc accgcgacgg ccgcgctcgc ctgcgtgccc
    15601 gtcaccagcg catgaccctt gccgagatag aggttgatcg cgctgccgtc tgccaccgcc
    15661 tgcacatcga cgaggccggc gagccgctcg atcagccggt cgcgctggtc caggaggtcg
    15721 ttgggctggt gcccgccggc gccgctgctg ccgacgcgca tggcaatctg ccggttcaac
    15781 tcggccacgc cgctcgcgag cgcggtgatt tccttcgcca gcgccgtgag ctgcgtgttg
    15841 agtccgtcgt tcatgtcctc cagctgcgca tagagcgact ggaagcgctg cgtcagcgtc
    15901 gttgccgagt ccagcaccac ggtgcgcacg gccgtcgact gcggatcgct ggcggcatcc
    15961 tgcagcacgc cgaagaaggc atcgatgcgg tccgatatgc cgatcgacga atccgcgaac
    16021 agcgcgtcga cctgctcgtt ccagccgttc agcgcctcgg cgcggaaata gagcgaggtg
    16081 tccgtgcgta gctggctggt cacgaactgg tcgacgatgc gcgagaccgt ggcgatatcg
    16141 acaccgttgc cgaacacggc gcccgagagg ttctgcccgg gcagcgacac cagctccgcg
    16201 cgctggcggc tgtagccctc cacgccggcg ttggcgatgt tgtggccggt ggtgttgagc
    16261 gagccctggt aggcgagcag gccggtcgtg gcgatgcgca gcaggtcggc catggctcag
    16321 cccgcccctg ctatcgcatc gtcggcgagc agctgcatca ccttgtgcgc gtatgcggga
    16381 tcggtggcat agccggcctg ctgcagcgag cgcatgaagc gcgccggatc gtcggcgttg
    16441 gcgagcgccg cggcatagcg cggctggccc ttcagcagcg cgatgtagtc ggcgaagctc
    16501 tcggcgaacg aaccataggc gcgaaaacgc gcgcgctgct gctgcgggat gccgttgctg
    16561 aactccagcg tcgtgacgcc gaccgagcgc ccttcccagt agctgccggc cttgatattg
    16621 aacaggttgt ggctgctgcg ccccgcgtgg tcgctcagga tcgactggcc ccacccggtt
    16681 tccagcgccg actgcgcgat cagcacgcga tgatcgatgc cgagcgcctg ggcggcccgg
    16741 cgcgcctcgg gcagcaccgc gcgcacgaag gcttccttgc cggaaaacag gccggcggcg
    16801 attttttcgc gcgcctgctg cgcgaccggc tccaggatct gcagcgcgct gctcaccacc
    16861 ggcggcagcg cggcgcgcgc cacgcgcggc agcagcgcag gcgccggctc gcgcggcgcg
    16921 gcgtcgatgc gccgcgcctc gagctgtgcc tccaccgagg tgccggcgcc tgccacggcg
    16981 ctctcctgcg tgccgaactg ctggcgcaac tgccgcccga gcatctcggc cagcccgagc
    17041 ccgcggccct gcgccagcga gacgccgagc tgctggtcga gcatgtcctg gcggaactgc
    17101 atctcgttgg tgttcatcgg gttgtcggcg gcgaagatct cgttggccga gcgcatgccc
    17161 ttgagcaact gcgacacgaa gaacgattcg aactgccgcg ccgcggcctg cagcgcggcg
    17221 ccgcggtccg cgcgcccgag cgtggtgatc gagtgcaggc ctgccaggtc ggtccatgcg
    17281 gcgggagccg cggtctgcgc cggggcgcct gccacgctca tgatcccgcg tcccggccca
    17341 tcgcgatgcg cccctcagat cacgatcagg tcggcgcgca gcgcgcccga ttgcttcagc
    17401 gcctcgagga tcgccatcag gtctcccggc gccgcaccca cgctgttgac ggcctcgacc
    17461 agctggtcga gcgtggtgac gcccgccagc ttccacatgc ggttgccttc ctggtcgatc
    17521 tggacgtccg actgcggcgt gacgaccgtc cgtcccaggc cgccgaaggg gcccggctgg
    17581 ctgacctgca ggttctcggt caccaccacc gtcatgctgc cgtgcgtgac cgccaccggc
    17641 tcgaccgtga cgtgattgcc cacgacgatg gtgccggtgc gcgaattgat gaccacgcgc
    17701 gccggcgcct cgcccggctc cacctcgagg ttctccagca gcgagacgaa ggacacgcgc
    17761 tggtcggcat cctgcggccc gcgcacgtgc accgtgccgg catccagcgc gttcgccaca
    17821 tccgagccca gcatcccgtt gatccgctcg gcgacatgct tggcggtggt gaaatcggga
    17881 cgaagcaggt tgaactgcaa ctcactgccc tgggtgaacg aagtctccac gccgcgctcg
    17941 acgattgccc cgttggcgat gcgcccggcg ttcacgacgt tgaccgtaac gcgcgagccg
    18001 tcctggccct cggcgccgaa gccgccgacg accagatcgc cctgggcgag cgcatacacc
    18061 tcgccatcga cgcccttcag gggtgtcagc agcagcgtgc cgccgcgcag gctcttcgca
    18121 ttggccagcg aggagaccgt cacgtcgatc gtctggcccg gcttggcgaa cggcggcagc
    18181 tgcgcgtgca ccgccacggc ggcgatgttc ttcaattgca ggcgcatgcc ctcgggcagc
    18241 gcgatgccga actggcgcag catccgcatc aacgactgcg aggtgaacgg tgcctgcgtg
    18301 gtctggtcgc cggtctcgtc gagtccgacc acgagcccgt aaccgatcag gtaattgttg
    18361 cgcacgcccg cgagcgcggc gatgtccttc agccgctcgg cgtgggcggc accggcgcag
    18421 gacagcagga ccagcgccgc ggcgccgagc cgggaggcac gacgcgccag cagggcacgc
    18481 aaccggagca gcgggattgc catgctcata gcggccacac gccggagttg aagaacttgc
    18541 ccagccagcc catgttgttc gactgcgcca gctcaccggt gccgctgtag ccgatgcggg
    18601 catcggcgag cctggtcgag ggcaccgagt tgtcgggcgc gagatcttcc tggcgcacca
    18661 ggccgctcag gcggatgtat tcctggccac gattgagctg cagccacttt tcaccgcgca
    18721 cgcgcagcag cccgttcggc gtcacctcgg tgaccgtgac cgcgatgctg ccgctcaggc
    18781 tgttgctctg cgagctctcg gcgttgccct ggaagtcgcg gttctgctgc acatcggttt
    18841 cgaggctgtg atcgccgccg gcaaagtcgt tgccgagcat ggtgccggcg ttgaactgca
    18901 ggtcgctctg cttgctgacc gcggtgtcgg catccttgct ggcctgggtg cgctcgatca
    18961 ggcgcacggt gatgatgtcg ccgacctgcg cggcgcgccg gtcggaaaac agcgaggaac
    19021 cgaaacgggc gtactggatc gcgccctgcg cgtggaagtc cggcgccggc gtgttggcgg
    19081 tgagcggtgc gtacgccgga tcgtcgggca tttcctgcag ccgtggcgcg gcgcagcccg
    19141 cgagcacgga tgccagcagc gcaacgagca tgatgccggc gcggcgcggt gccggcgtgg
    19201 agtcaatgaa gcgtgtcatc gcggcgtcct gttacaggtt ctgcgagatg tagccaagca
    19261 tctggtcgct ggtcgagatg accttggcat tcatctcgta ggcacgctgg gtcgtgatca
    19321 tgttcaccag ttcctccacg acttccacgt tcgagttctc caggttgccc tgctcgatgg
    19381 tgcccagccc gtcgaggccg ggcgtgccgt tggtggggct gcccgaggcc gtggtctcga
    19441 ggaacaggtt gccgccgatg gcctggagcc cggctgaatt gatgaagtcc gtgagggtca
    19501 gggagccgat ctgctgcgcc tccgcctcgc ccgggagcac caccgacacg gtgccgtcct
    19561 tgccgatggt gagggactgc gcaccctcgg gaaccgtgat cgcgggctgg atcaccaggc
    19621 cgtcggcatt cacgatctcg ccgtcgcggc tgaggtggag ctggccgttg cgcgtgtagg
    19681 cggtggtgcc gtcgggctgc agcacctcga ggaagccgcg gccgttgatc gccacatcga
    19741 gcgaattgcc ggtgacctgc aggctgccct cggtgaactc cttctgcgtc gagacgatcc
    19801 gcacgccggt gccgagctgc aggcccgagg ggagctggtt gtcctgcgac gactgcgcgc
    19861 cgggctggcg gcggatctgg tacagcaggt cctcgaaggt cacgcgatcg cgcttgaagc
    19921 cgaccgtcga gacgttcgcg aggttgttcg aaatcgtccc gagctgggta tcctgagcgc
    19981 tcaggcccgt cttgctcacc cacagtgcat tcatcatcgt caattactcc ttcgggtcct
    20041 cgcgcggcat caggaaactt gcaacaagcg tgccgcggcc tcggtattct catcgacgga
    20101 cttcatcaac ttcacctgca tttcgtactg gcgcgcgagg ctcagcattt ccgtcagggc
    20161 ctcgacggca ttgacgttgc tcccctcgag aaaacccgag accacgcgca cccgcgcatc
    20221 gaccggcgcg cgcgcgccat cgcgcaggtg gaccagcccg tcctcgcgct tgagcaggtc
    20281 ctgctcgggc gggttcacca gccggatgcg ctcgacactg gccagcgcct cgggaccctg
    20341 ccccatggca cggacggtga tcgtgccgtc ctggccgatc tccaccttgc tcgcatcggg
    20401 aatcgcgatc ggcccgttgc tgccgagcac cggcaatccg ctgccggtca gcagctggcc
    20461 gaacggcgtt atctgcaggt tgccgtcgcg ggtgtaggcc tcggttccat cgggtgcctg
    20521 caccgcgatc caccccgcgc cgcgcaccgc gacatcgagc tcgcggccgg tctcgcgcag
    20581 cgtgccgctg gcgaagtccg tggccgggcg ctcggtcagc gcatgcacgc gcgtcgggaa
    20641 gccatcgccg taccagatgc ccatgctgcg cgccgcgacg tagtcgcggc gaaagccgtc
    20701 ggtgctcgcg ttggcgaggt tgttcgcgtg caccgtctgc gccagcgtgt tgtgcttggc
    20761 gcccgtcatg ctggtgtaaa gcgctcggtc catcggtgct cctgtctcgc tgcgcgcgga
    20821 ccctgcgcgc cgcgcagggc cagcgcgtca gcgctatcgc tcagctgcgg atattgatca
    20881 cggtctgcgt gatcgtgttg ttggtctcga tggtcttggc attggcctgg aagttgcgct
    20941 gcgcgacgat caggttgacc agctcttccg atatgtcgac gttcgagtcc tcgagtgcac
    21001 cggcctggag agagccgaac acgccggccg tcgccacacc gcgcgtgggc tgcccggaat
    21061 cgaaggactc ggcccattgc gtgttgccga gtgccgtcag gccctgcgga ttacggaaat
    21121 cggcgaggat gatctgaccg aggatctgcg actggccatt ggtgtagcgg gcgaacagga
    21181 tgccgttttc cgagatgtcg gtgcctacca gctgacccgg cccgaagccg tcctggctca
    21241 ccgaatcgat gttgaagtcg tcgccaaact gcgtggagcc gctgatgtcg agcaggaagt
    21301 ccgacgtgtc cgcatcgacc acgccgttga aggtcggggt cgcggcaccg cccagcggtc
    21361 ccagcggctc ggcgttggaa tcgaacacgc tctggatctg caaggcgggc acccagtcgt
    21421 cgatcacgaa ctggttggtg gcgggatcga gcgtgccgtc ctcgttgaag cgcaccagga
    21481 aggcactgtt gtcgccgttg ggatccagcg gcgcgcccgc cgggttggcc gggttaccgt
    21541 cgttgaccag ctcgccgtcg atcgcgagcc acatgaacca gtcgttgttg accccggtct
    21601 cgatcgccgg gtccggctgg tccttgcgga aatacatcgt gcccacgtgc gggatgccct
    21661 cggagtcgta gagcgtcacc gacgaggcga agttgaaggt gttctggtcc gcgggattga
    21721 actccgggta gagcgccccg tcgaccgcgg cggccgcggc gtcgaggttg aagccgagtc
    21781 tgacctcgga agtcttgctc ggctgcagct tgaaggcctc gatgcgcagg tccacctcgt
    21841 tgcccaggtc cacctgtccg gcggagttcg ccgcgtagcc cacgagacgg ccaccggtgt
    21901 tgctcaccac gtagccgtcc ttgtccaccc cgaacacgcc cgcgcgcgtg taggtgcgct
    21961 cgccgccgtt gttcaggatg aagaaaccgc ggccgttgat cgcgacatcg aggtcgcgct
    22021 gggtgagcgc gatgttgccc tgcgtgaact gctgcgcggt gcgctgcagc atgacgccgc
    22081 tgccgatggt gctggtgccg gtgaggctcg cggcgtacac gtcgccgaac tccgcgcgcg
    22141 actgcttgaa gcccgtcgtg ctcgcgttgg cgatgttgtt gccggtcacg ttgaggtcgg
    22201 aagtggccgc gcgcaggccg ctgagtccgg tgttgaatga catgtcgctg ctcctcgctg
    22261 tatgtaaacc cgcagccggc gcgcgccggc tggccccctg tcagagaatt tccttcaccg
    22321 cgctgatgcc gagcggtccg atgccgtcga cgttcagcgt catctcgccg ttggccccga
    22381 tggtgacgct gttgacgttg gcgccgagcg cggtcgccag ccccacggtc tcgtcctcgc
    22441 gcaggcccag tgcctcgaag cggtagatcc ccggcgccag cgaggttccg gccgcatcgc
    22501 ggccatccca gccgaaggtg atgtcgccgg catcctgcct gccgagcagt tcctggcgca
    22561 cgagcacgcc ggccgggttg tagatgttga gcagcgtgtc atcgaccggt tcgtcgatgg
    22621 tgatcgtgcc catgatctcg ccgccgctgg cgagccgcgc ggtggaggcc tcgacgagca
    22681 ccgtgtgccc gaccagcgcc gaggcctgga tcgcctggct cgatttcagg ctgccgttca
    22741 tcgagacgaa attgctgttg aggtcctcga tgccctgcag cgtgctgaac tgcgcgagct
    22801 gcgccacgaa ggcggtgttg tcctgcggcg tgagcgggct ctggttctcg agctgcgtga
    22861 tcagcagcgt gaggaaatcg gcctgcccga gcgagctgtt ctccgccttg gcgggctcct
    22921 tcttgaagga cagcgcctcg taggcactgc cgctcacctt gaatccgttg atttcgctca
    22981 tcgttcaatc accccctgcc ctggtgtgcg gtgccgctca ctggcccagt gccagcacgc
    23041 gctgcgccag cgtcttggcc gcttccacca cctgcacgtt gatctggaac gagcgcgagg
    23101 cggagatcat gtcggccatc tcctcgacca cgttcacgtt cgggtagtac acgtagccct
    23161 gctcgtcggc gagcggatga tgcggctcgt acctgggttg cagcggcgcg tcgctctcga
    23221 cgatgcccag cacctgcacg ccggcctgcg catcgccctg cgccgttcca ccggacccga
    23281 gctggtcctg gtacagcggc gcgaacaccg gatggcgcgc gcgaaaggtc tgcccgatgc
    23341 tgctgctggc ggtctgggcg ttggccaggt tgctcgccac cgtgttcagc cggatgctct
    23401 gggcgctcat cgccgagccg gcgacatcga agatcgcgtt gatggacatc attccccctt
    23461 cagcgcggaa cgcaggccgc ggaacttgcc ctgcaggaac gtgaggctgg cctggaaatc
    23521 gagcgagttg cgcgcgaact gcgccatctc gatatccgca tcgacggtgt tgccgtccag
    23581 cgtcggctgc atcggggtgc ggtacagcag gtcgccggcg attgcgggcg cgccggcgcc
    23641 aatgtgcagc gcctgcgtgg cgcgcagcct cgacccgtcc tgctcccccg ccgcctggcg
    23701 gatcaaggcc tggaaatcga ggtcgcgcgc ctggaagtgc ggggtgtcgg cgttggccag
    23761 gttggcggcc agcacgccgg cacggcgctc gcgcaccagc agggcctgtt cgtgcacgcc
    23821 tagcgccgcg ttgaagttga ttgccatgcc gggtatccag tgcgttgtca gcctcgacgc
    23881 agcaatcggt gtgccattga atgcaagcca ttgtttttaa ttttctttat cgccgtccct
    23941 gggggagcgg caagcgtccg gcggcaaact ccttccgcta tgggtccggc cgcgccgacg
    24001 ccggcggcca aggcctgccg cgcgccgcgg cgccatcgcc cggcatcggc ggctgcggca
    24061 ccgcggcacg ggatttgctg gaaagccctg gacccgccat ctccgccgga attccgtcac
    24121 gcatgaccat gtccgcccgg ctccaccgac tgctgtacgc cctggcttgc gtgctggccg
    24181 ggctgtatac gctcgcggca cgcgcagagc cggccaccgt ggacgcgcgc gcgatcgcgg
    24241 cgctggtcga acactcggta ctcgcgcagc tgcgcgacgc gccgctggcg cgcggcgccg
    24301 cgcgcgtcga ggtggcggca ggcgcgctcg atacgcgcct cacgatggcc gcctgcggac
    24361 aaccgatccg tgtcgcggcg gacctctcgc ggcagcaggc gcgggtgaac gcgcgggtca
    24421 gctgcagcgc cccctcgccg tggaacctgt acgtgcccgt cgagatccgc gtgtaccggc
    24481 cggtgctggt cgccacgcgc gagttgcgcc gcggcgagcc gatcggcgcc gcggacgtcg
    24541 cgctcgagga gcgcaacgtc cttgccgccg cgggcgcacc gccgctcacg caggccgccg
    24601 atgtcgtcgg gctcaacgcc cggcgcacca tcgcccccgg cacgctgctt agcgccggcc
    24661 tggtcgagct gccggtgctg gtgcgccgcg gggaccggct cggcgtgagc gcccgttcgg
    24721 gaggaatcac tgtccagatc aacggcgaag cgctgggcac ggcccgcctc ggggagcggg
    24781 tacgggtgcg caacctgcaa tcgggccgcg tcatcgacgc ggtggtgacc ggcagcggca
    24841 gcgccgaggc catctgaggc gggcgccccg gaacccgact tgaaaaagga ctaaaggttt
    24901 actatgggcg gccgatacct tcatcgagcg cccacgctgg agtcagcagg gcattgcagg
    24961 tgacagtttt tatggtttcc gacatcaagg gattcgactc gcgccaggcc tccggcgccc
    25021 gcacgggcga aaccggggcc gtccggtccg actcgcgcgc aggcgcggga cgccaggcgg
    25081 gtgcggctgc ccgcgatgac accgtcgagt tgagcggggt ggcggatctc atcgccaccg
    25141 ccgcgcgcaa gctcgcggcc gagcccgccg tggaccaggc gcgggtgcag gaaatcagga
    25201 aagccatcac caacgggagc tacacggtag atcccgagcg catcgcccgg aagctgatcg
    25261 acgctgattc gcagtactga tccggtactg ccaccaccag gggcgatctt catgcagaac
    25321 ctcaccgacg atgccggcgt cgcacacgcc atcgtggccg cggatctcgc ggccgtgcgc
    25381 accctgcgcg aactgctact cgaggagcgg gcggccctga cccggcgcga gcccgaggcg
    25441 ctgaacgccg tggtgcagcg caagctcgcg tgcctcggcg agctgcagca gagcgaggac
    25501 gcgcgcaagc gcctgcttgc gcgccaccgc ggcagtgact gggcggcgct gctcggcacg
    25561 ctggacccgg cactggccgc accgtgggag gcattgcgcg ccaacctgca acaggtcgcg
    25621 gaactcaacc gcgtcaacga gcgcatcgtg gcgcgcacgc gccacagcac cgagcggctg
    25681 ctgtcgatgc tgcgcggcca gctcgacccg gtcggcgtct acgaccgcag cggccagacg
    25741 cgcgcctacg gcgacgtgcg cccgatcgcc agcgcctgag cggcgtcgcc gagacgccac
    25801 aggtcctgcg ggcgatcgat gtccgcgcgc ccgggcagtt cgtgccagcg cagtccgagc
    25861 gccgcgaagc gctcgcgctg gcgcgccagc accacgtccg ttccccacgg cactcccgcg
    25921 aacaccgcgc tcggcagccg cgtgaagccg gccagcacgt agccgccgtc gagcgcgggg
    25981 ccgagcacgg catcgaagcc gtgctccagc gccaccgccg catcgccgag gtaggccgcg
    26041 tcgtagccga ggcaatcgct gccgaccacg atcactgccc ggtattcggc gagcgcggca
    26101 tcgacggcgc gccacatgcg cgcgcccagg tccccgccgc cctgcgcgcg cagcgtccgc
    26161 ccctcgcgcg cggccagttc gcgcaggaaa gcatccgtgg tgtcgtccac gcacagcgtc
    26221 gcttcccagc cggcggggac cgcgcgcagc gccgcggcaa cccggcgtgt cagctcgcgc
    26281 gccacctccg cggcccccgc cggcgagagc tgcccgtgca gcctggtctt caccgcgccg
    26341 gcgcgcggcg ccttggcgaa ctgcaggaaa cacccgcacc ggcgccggtc ctcagcgttg
    26401 gccatggtag gtctcgtgca ggtccgcggg atcggcgccg aggaagtagc tcaggcgcag
    26461 gcgccacatc agcaggatcg tggacagcgc gccgtcgcgg tcccagcgcc ggccggagct
    26521 ctcgataaac gccgggtgcg cgagcggcgc gccgtggcgc cgcaggcggc ggcagatctc
    26581 gatgtcctcc atcagcggtt gcgcgggata gccgcccacg gcggtgaaca gcgcgcgcgg
    26641 cacgaacagc ccctggtcgc cggtggcaat gccgctgagg cgcgagcgcc aggccatgca
    26701 gcgcgcgacc acgccgagcc agcgcgagac accgcgcagc cgcacgcgaa aaaagcccca
    26761 gtgcggcgcg gcaccggcca gcgccgcgat cgccgcgacg cagggcggcg gcacgcggct
    26821 gtcggcatgc aggaacagca gcaactcgcc gcgcgcgcgc gcggcgcccg cgttcagctg
    26881 cagcgcgcga ccgcgcggcg ccgcgagcac gatgtccgcc agcgcccccg ccgccgcgac
    26941 ggtgccgtca ttgctgccgc cgtcggccac gatgatctcg gcgccgcgtg cgcgcagcgg
    27001 ctgcaggtcg cccagcagca gcggcaacgc ggactcctcc tcgagcgccg ggatcacgat
    27061 gctcaggcgc atgctcaggc ggccagcgct cccgagcagc tgctgccctg ccccgccgtg
    27121 cagccgtagc agtgcgcgcc agtgacgatc ggcgcatcga gcggcaaccg ctcgagcaga
    27181 tcacgcaggt gcaggcggcg gtcagtggcg agcagtggca gatcgagcat ctggttgaaa
    27241 tcgcagtcgt agacgaagcc acgccagtcc acgctgagca ggcgacggca catcacctgc
    27301 ccgaggttcg cgtgcacgaa attgcgcttg agcagcgcga ggtagtcatc gaactgcccc
    27361 tgcgccagca gcaccgcgcc gaagcgcgcg accggcaggt tggcgagcgt gagcaggcga
    27421 tcgaagccga tgccgtagcg cacccgcagc tcgcgcttgt aatcgagctc gagcgcctgt
    27481 tgcggcggcg gcagcgtggg cccgagcggg ttgtagacga ggttcaactc cagcgcgccg
    27541 ccagcgccat agccgagcgc gttcagccgc cgcagcgccg cgatcgagcg cgcgtgcacg
    27601 ccgcgcccgc gctgcgcgtc cacgttggat tcgaggtagc acggcaacga agcggtgatc
    27661 attacgccgt gatcggcgag gaactgcgca aggtcctcct gcccgggctc ctccagcacg
    27721 ctcaggttgc agcggtcgat cacgcgcagt ccgagcgccc gggcccgcgt cacgaggtga
    27781 cggaagtgcg ggttcagttc cggcgcgccg ccggtgagat cgagtgtcgt gacgccgtgg
    27841 cgcgcggcca cttccagcag cagatcggcc gtatcccggt ccatcatctc ggtgcgtgac
    27901 ggcccggcgt tcacgtgaca atgcgcgcaa ctctggttgc agcggtagcc gagattcgcc
    27961 tgcagcgtat cgacgcccag cctgcgcatg gccgggaagt ccgattcgag caacagcggt
    28021 cgcgtgtcct gcatggcgtg gatccggatt acgatggtcg ggaactgcga aggacagcac
    28081 gattccggca ggcacgcaac cacgcgcatt gccgtggcac gccgatctgc cagactccgg
    28141 gaagcgccac acaaggaggt acagccgatg ccattcccgc cgccgcgtgg agccgcaccg
    28201 tgagcccgac ggaacagttg ccgcccggcg cggagcggcg caccgagacg cgcatagaag
    28261 tcgagttgcc ggcacggatc atcgcccgcg acgggcgcgt gtacgcggcg gccatacgca
    28321 acatctcctg cggcggggtg ctggtgcatc tggcggccgc gcatgccgcc gcactgctgc
    28381 cgaacacggc gcgtgaacaa ccccgcctgc cggtcggcgt gcgcctcgaa ttccagcttc
    28441 ccgccgcacc gcacgacgcc cccgtggcca tcgactgcgg catcgctcac ctgcgccgcc
    28501 tggcggcgga ccagggcgcg atggggctga actttcgcca cttccatgcc ggcagcgaac
    28561 acgcactggc ccgcttctgc gcgatgtagc cgcgcgatct cgcgcattcg cgccaccgag
    28621 gtggccggct cgggccgaag tcattagaat gcgccgtccc gcgcggccgc gtccccatag
    28681 ttcaatggat agaacggggt cctcctaaga ctcagatgca ggttcgattc ctgctgggga
    28741 caccaaatcc cgcgcgcgcc agcctcgccc gctttccgcc gccctcggag cgtcgccggc
    28801 gcgacaccgg cttatggcgg cattaatcac agtcggccgc tttgggccat gcaccccacc
    28861 ccggtttttt tataatccgc cgaacgcaat ccccgggcga tcggtcgccc ctccggtatg
    28921 aacgaagcag tcaaagacgg cacccagaac gtcctcgatg gcaaacccgt gatctattac
    28981 gacgggtact ggatccgcta ctatgccccg cccgaaaaca cgctcccgca aaagaaggcg
    29041 ctcatcgacc agatgaccaa gcgcgccttc caccacaccg aggaaggcat caacacgccc
    29101 gggcgcaagc tcgaccgcgc gcgcgcggcc tgggagagcg agaccgaccc cgcgcgcaag
    29161 cgtgtcaacg ccgcgatgct cgccggcgca ctgttcaatc gcgccaccga cctgttcacc
    29221 acgatcgtgg agctcggcga gatgggcgtc gagatcagca tggacaacga gttgatgaag
    29281 cagtgcggcg agtgcttcaa ggaggcgctc gatctgggca agtacgtcaa gcactacagc
    29341 ggcgaggaag gcatcgacga actgtggggc gagccgttca aggccttcac gctgccgatc
    29401 gcgacggtct acgagagccg ctacatgaag gtcgcgcgcg cgatgcgcga catcgacggc
    29461 ctcgccgaga agatggagca ggtattcggc ccgctggcgg gcttcgcggg aatcgccgag
    29521 cgcatccgcg aactcgccgc cgcggccaag ctcgaggcgg agaccatgaa gtccgacccg
    29581 gtgatctttc gcgtgtggcc gcgcttcgtc gccgcctgcg aagcggtgct cgacttccag
    29641 ccggcgctgc ccgcgccggt gagcgaccgc gagcggcgcg aggccgacga ggcgctgcgc
    29701 atcctgtgcg agggcaagcg cgtgatcgag tacctcgccg gggcgcgcgt cccgatgccc
    29761 aagaccaccg cggcctacca tcgccgctgc gacgccttcc gcgagtccat cgcccggaca
    29821 tgaagcagct cggcgtcctc gactccgcct tcatcaacct cgaactgccc ggcacgccac
    29881 agcacattgg cgggctgggg atctacgacc cgtcgaccgc gcccggcggc ttcgtgcgct
    29941 tcaagtccgt gatcgccggt ttcgagcgcc gcctgcggcg cacgccgcag tttcgcgcgc
    30001 gattgcagca ggtgccgggc ggcatcgacc gcccgtactg gatcatcgac gaggacttcg
    30061 acctggagtt ccacgtgcgc cacatcgcgc tgcccgcacc cggcgactgg cgccagctgt
    30121 gcatccaggt cgcgcggctg cacgccaggg cgctggattt cagccgcccg ctgtgggagg
    30181 cgtggatcat cgagggcctg gacaacctgc ccgggctgcc gcgcggcagt ttcgcgatct
    30241 acggcaagct gcaccacgca ctgttcgacg gcgccggcgg ccaggtcttc gtcgatgcgc
    30301 tgcacgacct cacgccggta cccgccgagg cggcgcccgc gtgcgcgcag gaccgcctct
    30361 acgccgacga agcgctgggc gaggtgcaga tgctgggacg cgcggcgatg aaccagctgg
    30421 tcaacggcat cgacatggcg cagggggcgc tgcgcgccgg cggcgagatc gcgcgcatgc
    30481 tgctcgggca cggcgaggac ggcggcaagt cgctgctggc cagcgccccg cgcacacgct
    30541 tcaacgagcc ggtcggaccg cgccgcagct tcgaggcggc cagcttcgcg ctcgcggatt
    30601 tccgcgcgct gaggaatgcc accggcgcca ggctgaacgc gctggcgctc accgtgatcg
    30661 gcggcgcgct gcgccgctac ctcggggccc gcgcggagct gcccgagcgc gccctgaacg
    30721 ccgcgattcc cctggacctg cgccggcgcc gcgccgattc cggcgacacc aaccaggtcg
    30781 gcaccgtgta cctgtcgctg cataccgacc tcgcggatcc gctgcaacgg ctggaggccg
    30841 tgcgccgtga agccgaccgg gcgcgcgagc gcggcgcccg caacccgctc gtggacacca
    30901 tgaaactcgc cggcctgctg ccacccgcgg tggtcagggc ggccagcgaa ttctatgccg
    30961 cgcacggcct gacgcgacgg atgcccctgg tcaccagcac cgtggtcgcg aacgccgccg
    31021 ggcccgagat accgctctat tgcgcgggcg cgcgcctggt gcgctaccac gggttggggc
    31081 cgctgtcgcc ggggatgggg atctaccaca cggtgttcag ctgcaacgat acgctgacga
    31141 tctcggtgct cgccgatcgc gagcacatgc ccgaccccgc gttctaccgc gaatgcctgg
    31201 aggaatcctt cgccgagctg cgcgacgccc tgtaggttga tggcattcat gccgacgatg
    31261 tgcattgccc cgatgccatg gtcgccatga atggcgacct acgtgccgcg ggccaccggg
    31321 gcgccggggc gcgaacacca atcgctccag ctgccgggat agagccgcgc gccacggatg
    31381 ccggcgagct ccagcgacag caggttcgcg caggccgtga cgcccgagcc gcaatacacc
    31441 accagttccc gcgcatccac gccgtgccag cgctcggcct gcgccgccgc atcgaggaag
    31501 cagccgtccg cttcgcgcgt ggcgcccgcc caggggcggt tcagcgcgcc cggcacgtgg
    31561 ccggccaccg gatcgatcgg ttcctcgagg cccgcatagc ggcgcggctc gcgcgaatcg
    31621 accagcgcca gcgtgccggc ggcaacgcgc ggcgccagtt cctcgtaatc gatgcgcatc
    31681 gcggcgcgcg ggcgcggctc gaaatcgccc ggcgccggcg ccgggatggc ggtatccagc
    31741 gccagcccgg cgcgggtcca cgccgtcacg ccaccgtcga gcacgcacac gcgctcgtgt
    31801 ccgaaataac gcgcgagcca ccacagccgt gccgcgaagg cgccgcgcga atcgtcgtac
    31861 gcgacgagga ggcgcccggc atccagcccg atgcggcgca tggtccggcc gaaggccccg
    31921 ggcgagggca acgggtggcg gcccccggtc gctgcgacgg gcgccgagag atcacgctcc
    31981 aggtggaggt agaccgcgcc ggggatgtgc gccgcgcggt acgcagcctc ccccgccgcg
    32041 ggatcggcga gcgcgaagcg gcagtccgcg accagcggcg gatgcgcgcc ggcaagcagc
    32101 gcgtgcaggc gcagcgggtc gatcagcggt ccggtgagcg cgctcatggg ttctccttgc
    32161 ctcgcggcgc cgggttcagg gcctgcgaca gtttcaccgg cctcagcgca ccgtgaaacg
    32221 cagcgtgccg agcgcgccgc aatccgcaac gtagtcgccg ggctcggccg gcaccatggc
    32281 gcccaccgcg ccgctcagca gcacctggcc cgggcgcagt tcataaccgg ccgccaccgc
    32341 ggcattcacc agctccagcg ccgcggtcag cgcgccgtcg gcatggccct gcgccaacac
    32401 cgtggcgccg cgtgtcagca tcagcgccgg tgccgcgccc tgcagcaggg ccggatcccg
    32461 caccgcgccg aggaggtagc cgcgcacgcc gatattgctc gcgatgtagt cgatcccgtt
    32521 cggcggcctc accttggcct cgagcgcgag ctccggcagt tcgaccgcgg gcatcacgcc
    32581 gtcgatgcgt gccagcagcg ccgcctcgtc cgccagcggc tgcgtaatcg cggctccgag
    32641 ccgcaacgcc acctcgatct ccagcaccat gcgttcataa cctgcgcgcg agaccgtgca
    32701 gccggcggcc tgccccgcgc aacgctcggc cgaggcgggc aataccgcca aggccgcacg
    32761 atcgagaccg aagcgctgct gcatctgcgg cgaattgaag gcgctcttga agccggccac
    32821 gcgctcgccc acgggccacg cggcgaccag tgcctgctgg cgcgccatcg cctcggccag
    32881 cggatcagcc accgccacgc ccgcggcaag gcaaaaccac gccaaagcga gcaacagcgc
    32941 gccgggcgcg ggttgcagga cttcacgaaa gcggttgctg cgcggcattt gtacgcttcg
    33001 gtagaatacg cggcccccgc ctgcaccgag acccgccatg aaccagccca aagtcggatt
    33061 cgtcagcctc ggctgcccca aggcgctggt cgactccgag cgcattctca cccagctgcg
    33121 cttggacggc tacagcatcg tgccgagcta ccaggacgcc gacgtggtgg tggtgaacac
    33181 ctgcggcttc atcgacagcg ccaagcagga atcgctggac gccatcggcg aggccctcag
    33241 cgagaacggc cgcgtgatcg tgaccggctg catgggcgcg caggccgagc agatcatgaa
    33301 ggtgcacccg caggtgctca gcgtgagcgg accgcacgcc tacgaggcgg tggtcggcgc
    33361 ggtgcaccag cacgtacccg cgccggatcg ccagcacgat cccttcgtcg acctggtgcc
    33421 gccacagggc gtgaagctga cgccgcgcca ctacgcgtac ctgaagatct ccgagggctg
    33481 caaccaccgc tgcagcttct gcatcatccc gcagctgcgc ggcgacctcg tgagccgccc
    33541 gatcggcgag gtgatggacg aggccgagcg cctggtgaag gccggcgtgc gcgagctgct
    33601 ggtgatctcg caggacacca gcgcctacgg cgtcgatctg cgctatcgca ccggcttctg
    33661 gcagggccgc ccgctcaaga cgcagatgca ggcgctgtgc agcgcgctcg gcgagctcgg
    33721 cgtctgggtg cggctgcact acgtctaccc ctacccgcac gtcgacaaca tcatcccgct
    33781 gatggccgag ggcaggatcc tgccctacct cgacatcccg ttccagcacg cgagcccgcg
    33841 catcctcaag ctgatgaagc gccccggcaa cgccgagggc gtgctggagc gcatccacgc
    33901 ctggcgcgag acctgccccg acctcacgct gcgcagcacc ttcatcgtcg gcttccccgg
    33961 cgagaccgag gccgaattcg aggaactgct ggacttcctc gacgaggccg aactcgaccg
    34021 cgtgggctgc ttcaagtact ccccggtcga tggcgcgccc gccaacgcgc tgcccgatcc
    34081 ggtgcccgag gaggtcaagg aagagcgctg gcagcggctg atggagctgc aggccgagat
    34141 cagcgccgag cgcctgcagc agcgcatcgg ccgcgagatc gacgtgatca tcgatcgcgt
    34201 cgagggcgac agcgccatcg cgcgctccag cgccgatgcg cccgagatcg acggcgtggt
    34261 gcacatcgag aatgtcgagg gactggtctc gggcgagatc atccgcgtgc gcgtcgaggg
    34321 cgccgacgac tacgacctct gggccgtgcc cgtcgaggca tgaacctgct gctgctggcg
    34381 gcggcggaga tcgccgcccc gggcctggcc gaggtcgcgc tgtcgcgcta ccccgctcgc
    34441 gccggctggt ggccaccgca gcccgggcgc cggctgctgg tgggactgcg cgacggactg
    34501 ctcggcgagg gcgtggtcga gcacctcggc caacgccacg cgcgcatccg cttcacgctc
    34561 gatcgcgcgc ccccgcccgc actgccgctc acgctgctgc tcgcgctgcc gcgcccgaag
    34621 atgctgcgcc gggtgctgcg cagcgcggcc gagatcggca tcaagcacat ctggctgatc
    34681 aacagcagcc gcgtcgagaa gagctactgg cagagcccgc tgctcgcacc gccgatgctc
    34741 gacgcctatc tcgccgccgg cctggagcag gcctgcgaca ccgtgctgcc gcgggtggaa
    34801 ctgcgccagc gcctgcggcc tttcgtggag gacgagttgc cggcgatcgc gccgggcagc
    34861 ctgcgcctgg tcgcgcaccc ggcggccggc gcgccgctcg gcccggccgg cgccggccac
    34921 gtgacgctgg cgatgggccc cgagggcggc ttcacggatt tcgagatcgc gctgctcggc
    34981 gccgccggtt tcgccccctg cacgctgggg ccgcgcgtgc tgcgcgtcga gacggcgctg
    35041 ccggtgctcg ccgccaacct cttccccgcc gggttcagta atgcagcagc ggatgacgca
    35101 acgtgtcgtc cgccagcaac tgcgcacccg tgaattcctg gcgcaggtcc tcgggcgtga
    35161 gcaggctcag cagcagccgc ggctcgccca cgtagggcgt ggcgcccacg gtgttcaccg
    35221 tgtacaggta actgcgcacg tcgccgtcgg gcatcgccag gcgcaacctg aagctgttct
    35281 ggaacgcgat ttccgcgccg cggcgcgccc actgctccgg gatcgcggga taggccagca
    35341 attgtccgag cagccgcgcg gcatccgcgt cgccgctcag caacacctcc tgctgcagcg
    35401 tcatcagcag ccggcaggcg acgtcctccc agtcgaccag gcgcgcgcgc aggccgttct
    35461 cggcgaagaa catgtggtag ctgttgatcg gcggcgcgag gaactcgggc gcgagcagcg
    35521 cgcgcagcat cgccagccag ccgcggttga cgaggtgcag gttgccgtac ttgtcctgca
    35581 ccgtcgcggc ccacggatcg agggcgcgca tgttcagctc cagcgatttg tgcagccagc
    35641 gcatctcggg cgcctgcatg tcgacgcccg gcgcatcggg cgcgaaaccg gcggcgacca
    35701 gcagcgtgtt gagatcctgc ggcgcgaggc ggaacacgcc cgccagcgcg agcaccatgc
    35761 tgcgccccgg ctgcgagcgg ccggtctcga ggaaactgac gtggcgcgcg gacacgccgg
    35821 tctccagcgc cagctcttcc tggctgagac cgaaggtggc gcgccagaag cgcagcagcc
    35881 gcccgaaggg gccgggctgg tgaagggagt tgctcgggta ttcgctcaca gtgccggtcc
    35941 gtgtttcgcc cgatactacc cgcggggtaa tgagctgtac acaacgccac ggggctcgat
    36001 ccgccttggt gcaatagtgc aaccgtcagt ggcgggcgcg cggcacgcag gtctacactt
    36061 tcccgcaggc aaaagtgtga cactccaatg acaacaaccg cttcgccccg ccccactccg
    36121 ccccggctgg cctatgcgct gaccgagccc ggccgcttcg tcatcgagat caaccggctg
    36181 ctgctggcgc acccgttcct gcgccgcgcg cgcggcggcg acgggcaccc ggtgatggtg
    36241 ctgcccggct tcctcggcgc cgacggctcc accgcggcgc tgcgctacta catcgccaac
    36301 tggggctacg acacccacgg ctgggccatg ggacgcaaca tgggcctgcg cgcggaagac
    36361 cccgaattcg agaaccgcct ggccgaacgg ctcggcacgc tggtgcgcaa gagcggacgc
    36421 aaggtgagcc tggtcggctg gagcctggga ggcgtgctcg cgcgcgagct cgcacgcgcc
    36481 tgccccgacc acgtgcgcca ggtgatcacg ctcggcagcc cgatcggcgg cgatcccaag
    36541 gccaccacga tctggcgcgt ctacgagctc gccaccgaga ccgagctgca cagcgaggaa
    36601 ctgcgccagc gcgtcgcgca gatcgtgcaa ccggtgccgg gcgtgccctg caccgcgatc
    36661 tacagccgca gcgacggcat cgtctcgcac cggatcgcgc gcgagcgcga gacgccgctg
    36721 accgacaaca tccgcgtcgt cgcgagccac gtcggcctcg gtttcagcgc cacggtgctg
    36781 cacgccatcg ccgaccgcct ggcgcaggac gagaacgact ggcgaccctt tcgcaggacc
    36841 tgctggcggc gtatcttcta cgcctgaacg cgccggcctt ccgccgcgcc cgcgacggaa
    36901 gcacaggatg aagcgcgagg gcaacgcccg ccaggtactc agcgtcagcg acctcaaccg
    36961 ccacagccgc gagctgctcg aagccgcgtt cccctcggtg tgggtcgagg gcgagatctc
    37021 caacttctcg gcaccgggct cgggccactg gtacttcacg ctgaaagaca gcgatgcaca
    37081 ggtgcgctgc gcgatgttcc gcaaccgcaa cctgcgcgca cgcctgcgcc ccgcgcacgg
    37141 cgaccgcgtg ctggtgcgcg gcagcgtcag cctgtacgcg gcgcgcggcg actaccagct
    37201 gatcgtcgag gagctcgagg ccgccggtgc cggcgcgctg cagcgcgcgc tggagctgct
    37261 gcgccagcgc ctcgccgccg agggcctgtt cgcccccgag cgcaagcgcg cgctgccgac
    37321 cttcccggcg cacctcggag tgatcacctc cgccaccggt gcggcgctgc acgacatcct
    37381 gaccgtgttg cgacggcg
//
LOCUS       DAORUQ010000010        34723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_792_length_34723_cov_6.578084, whole genome shotgun sequence.
ACCESSION   DAORUQ010000010 DAORUQ010000000
VERSION     DAORUQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 34723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34723
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_792_length_34723_cov_6.578084"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLW13_01910"
     CDS             complement(<1..1100)
                     /locus_tag="PLW13_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY69151.1"
                     /translation="MLGISLATAVLADTEARVQFAVPASTLPAALIELARQAQLSLVF
                     ATANVPSNPTVAITGTLSPRQALERLLEGSGLVPAYLGDNVVSIGPPCNSAADCAPAA
                     AQPGTEAERYVDARVIEQIIVREQLRTGSHIKQANYLGSGPVHVLGAPEIEASGAQSL
                     GDLLRFMPAVIGNQTSTAVSNGGDGTATVTLRGLPAGNTLVLVNGRRSSAGGLAGDAF
                     DLNTLAPAAVERIEILKEGASAVYGSDAIAGVVNIIMKSGYDGVQLGTQYGSTQRGDR
                     ATRSTSLLAGNTWSRGSLLFAATVFEQDPLWSRDRAVSRHADGRPRGGADLRSSATPA
                     ARFDVDGNTLTLDGTADGTRPGVQKCRQGLQI"
     gene            complement(1270..2223)
                     /locus_tag="PLW13_01915"
     CDS             complement(1270..2223)
                     /locus_tag="PLW13_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQY69152.1"
                     /translation="MTRTTPAQHQALDQAAAWIARLRADDVSAGDRARFACWLSEDAG
                     NAAAFDRMLELWSDLGVLRALPIEIPRTARARRWQLPLGLAAAAAVAALLVLPRGAEP
                     PALELRTPVGGFQQLRLADGSEITLNTDTALSVALRDETRTATMDHGEVFFSVAPDAA
                     RPFSAVCGEASVTVLGTAFGATCDSGGMSVVVTHGRVRFATRVGAGASRELGAGDAVR
                     YDRQDNLAEISRVDADKALAWQQRRFVFEDVPLAEVIGELQRYMEPALRLADPAAGTI
                     RVSGVFSTAEPRLTLAALEQSLGLAVTGPEQGPLLISRRTD"
     gene            complement(2396..2965)
                     /locus_tag="PLW13_01920"
     CDS             complement(2396..2965)
                     /locus_tag="PLW13_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY69153.1"
                     /translation="MPPPDSPEESRTIDTRDIMAKRPGELLETLISSQRSALVRFLSR
                     KLGSIDDAQEIAQDAFMRLHRLEHADDLENARAYLFQVASNMAIDQLRRRKLHARYLE
                     EEGARLQDDTAQQDPGTPEELVAARQQLGLIYRAIEDLPLRCRQALMLHRVRGLSYAE
                     IARDMGVSVSSVEKYILEALKHCREHLIP"
     gene            complement(3177..4133)
                     /locus_tag="PLW13_01925"
     CDS             complement(3177..4133)
                     /locus_tag="PLW13_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69154.1"
                     /translation="MTPLLDIRALHTHFRTARGQTVRAVDGVSLTIARDTVTGLVGES
                     GSGKSTLGKTLVGLLAPSAGEILYAGRPLPRRHRPADFRRQARAIQMIFQDPYASLNP
                     RMTVGEILCEPLRLQDGGARREHRELAAHWLERVGLTAGHMGRYPHEFSGGQRQRIGI
                     ARALIPGPEFVVCDEPISALDVSVQAQIVNLLEELRASLGLTLLFIAHDLAMVRHLSQ
                     RIAVMYLGRLVEEGPADALWSAPLHPYTQLLVAANPEPDPRAARARAHVPASGEPPSP
                     LDAVPGCGFATRCPLATGRCHGEAPQLRPVAEGRLAACHLLS"
     gene            complement(4130..5137)
                     /locus_tag="PLW13_01930"
     CDS             complement(4130..5137)
                     /locus_tag="PLW13_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69155.1"
                     /translation="MSAEAVPVLAVDGLEVEFATRAGTVRAVRGASYAVAAGRTLAVV
                     GESGCGKSVTLQAVMGLIDTPPGRITGGSARLLGRELLGLPARELDRLRGATVGMIFQ
                     DPMSALNPLMRVGEQIAEPLVAHRGMSRRAALARAVELLERTRIPAARERARQYPFEF
                     SGGMLQRVMIAIALACRPALLIADEPTTALDVTVQDQVLGLMQEIQREDGMAIVLVTH
                     DLGVVARMADDVAVMYAGQVVEHGPVDEIFYRHAHPYTAGLLQALPGAARARGQPLQP
                     IAGSPPDLRAPPPGCAYAARCPRAMRICDRLEPPGFALAAGHGARCWLHHRAAADGSA
                     R"
     gene            complement(5134..6495)
                     /locus_tag="PLW13_01935"
     CDS             complement(5134..6495)
                     /locus_tag="PLW13_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69156.1"
                     /translation="MSSHVPLPGDFLPLAAGVAQPLAATPSPSYREHVWARLRADRRA
                     MLSLAVIATLIIGALAGPWLWRVDPALQRLDEASRPPGPGRAALLVADPGPVAVPCAP
                     ETPAGLTVCGTPHTAAVRLAWHPVAGADAYAVYRHELAPHDREDLGVPLGEVVTPAFE
                     DRLGLEARNYHYSVVPLRAGLEAGAAITLRVEPRQAIAWSAALRRGLLAANAGHERIG
                     GELRLPAHPLGTDYLGRDILARIIHGARTSLFIGIAAPLLFVLAGLLYGGLAGYLGGR
                     IDNAMMRLADFVVALPFLLFMILFKVAFGIRPGESGVMPMLLAMVLLGWPASARLVRG
                     QVMAIRDEPYVQAARLMGAGTPYLVLRHMVPNVLGVLLVALSFAIPSAIFTEAFLSFI
                     GLGVAPPTPSWGSLCNDGIRSMLAHPHELAVPAAFISITVLAFNLLGDGLRDALDTRA
                     EPA"
     gene            complement(6521..7462)
                     /locus_tag="PLW13_01940"
     CDS             complement(6521..7462)
                     /locus_tag="PLW13_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69157.1"
                     /translation="MLRFILGRLCYGLLTVWFIATATFAGMHAVPGDPLLDARAVSPE
                     IRRNLEARYGLDQPLALQYLRYLGNMARGDFGVSFTQQNREVNDIIREHFPVSALLGV
                     LAIGFAALGGVFWGVITARTAGRWPDALVMFLVVLSISVPGFVLAALAQLGVLRLNAW
                     AGVSLLPVAGWGGPAHMLVPALVLGLGTMAFLTRLMRTSMLEVRGAAYVRTARAKGLS
                     PARILLRHQLPNAILPVITVLGPAIATIATGGFVVELVFAIPGLGRYFVQAVQQLDYT
                     VIMGTTVFYGAFLVFMVILVDIAYGLVDPRIRMAQAR"
     gene            7559..8053
                     /locus_tag="PLW13_01945"
     CDS             7559..8053
                     /locus_tag="PLW13_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY69158.1"
                     /translation="MKHLIIVRHAKSSWADPGQRDFDRPLNGRGERNAPEMAGRLKAR
                     GLRPQLIVASGARRAHATAKLMAEVFGYAKADIEIVDALYEASPRVWLEQIGALPAGL
                     DTVMMVGHNPEITALVAQLCPQARIDNVPTCGVLCLDYEAGDWAGIARAKPVSWEFDY
                     PKKP"
     gene            8095..8805
                     /locus_tag="PLW13_01950"
                     /pseudo
     CDS             8095..8805
                     /locus_tag="PLW13_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
     gene            9022..9696
                     /locus_tag="PLW13_01955"
     CDS             9022..9696
                     /locus_tag="PLW13_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQY69159.1"
                     /translation="MIGSGGYCHARVSGERIGLPVGKVVCVGRNYAAHAAELGNAVPE
                     VPLLFIKPPSAVVDMAPQIRIPGGCGAVHHELEIALLIGRELRAATPEEAWAGIAGVG
                     LAIDLTLRERQDQLKAKSHPWEIAKGFDGACPLSPFVPLDPALDLAALETRLVVNGRR
                     RQHGNSAQMLTPIIELLCYASRQFSLWPGDVVLTGTPAGVGPLAPGDRITAELKGLVV
                     VHAEIV"
     gene            complement(9701..10375)
                     /gene="rpiA"
                     /locus_tag="PLW13_01960"
     CDS             complement(9701..10375)
                     /gene="rpiA"
                     /locus_tag="PLW13_01960"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQY69160.1"
                     /translation="MKSQDELKQAAAAAAFAYVRERLDERSVIGIGTGSTANRFIDLL
                     AGVRAQVTATVASSEASAARLRGHGLPVFDLNVVDGIDIYVDGADEANAALQLIKGGG
                     AALTREKIVAAVAREFVCIADESKFVPVLGGFPLPVEVIPMARSHVARELVKLGGNPV
                     YRQGVTTDNGNIILDVYDFALPEPLKTEAAINNIVGVVTNGIFAHRPADLLLLAGANG
                     VRELRA"
     gene            10536..12071
                     /gene="ilvA"
                     /locus_tag="PLW13_01965"
     CDS             10536..12071
                     /gene="ilvA"
                     /locus_tag="PLW13_01965"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HQY69161.1"
                     /translation="MPKSYIKRILEARVYDVAIESPIDEAPQLSARFGNRILLKREDL
                     QPVFSFKLRGAYNKIVGLSAPERERGVITASAGNHAQGVALSAQRLQIKALIVMPRTT
                     PDIKVMAVRRRGARVVLHGDTFDEASKHAYELAEQKGMSFVHPYDDPEVIAGQGTIGM
                     EILRQLSGPIDAVFVPVGGGGLLAGVAAYIKYVRPSVRVIGVEPEDAACLDAALKAKR
                     RVVLSQVGIFADGVAVAQIGKETFRIIRQTVDEVITASTDEICAAIKDIFDDTRSITE
                     PAGALALAGLKKYVQRTGVQGQTLVAIDSGANTNFDRLRHISERTEIGEQREAVLNVT
                     IPEKPGSFRAFCNAIGRGRSITEFNYRYSDAGAAQIFVGVQVSPERNDREALLAELRG
                     KGYEVMDLTDNEMAKLHVRYMVGGRAPSIGDAEIVYRFEFPERPGALLNFLNRMEHDW
                     NISLFHYRNHGAAYARVLVGFQVPEGDRGALAEFFAEMTRRHRVYSYWDETDNPVYRA
                     FLR"
     gene            complement(12074..12661)
                     /locus_tag="PLW13_01970"
     CDS             complement(12074..12661)
                     /locus_tag="PLW13_01970"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQY69162.1"
                     /translation="MSARSTLRRQCRDARAALGATGRARASQAICRHLGESPLLHAAS
                     RVALYWPVGEEVDLRPLLADDRHAHKQFCLPVMQPDKRLRFARYRQGDALHTNWYGIP
                     EPVHETAQEIAADAIGLVCVPLLGFDRAGNRLGQGGGFYDRSFDFLLRRAAAAPLLVG
                     IGFACQELPALAREAWDVPLAAVMTEDGLIDCRAR"
     gene            complement(12706..12884)
                     /gene="ssrS"
                     /locus_tag="PLW13_01975"
     ncRNA           complement(12706..12884)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLW13_01975"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(12931..13245)
                     /locus_tag="PLW13_01980"
     CDS             complement(12931..13245)
                     /locus_tag="PLW13_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQY69163.1"
                     /translation="MSAELQTVRVHILDKDYQVACPAEQRDALVESARYLDQQMRAIR
                     QSGKVIGLERIAVMAALNITHELIQRGEQSDKGSQDVHDRLRRLSARIDDTLNNYRQI
                     EM"
     gene            complement(13242..13451)
                     /locus_tag="PLW13_01985"
     CDS             complement(13242..13451)
                     /locus_tag="PLW13_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HQY69164.1"
                     /translation="MEGQQLKALEQRVDDLIRLCARMDQEIKTLKAAERMLRDERAQL
                     LRKNEDARSKVEAMIVRLRSLEQES"
     gene            13566..14111
                     /locus_tag="PLW13_01990"
     CDS             13566..14111
                     /locus_tag="PLW13_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HQY69165.1"
                     /translation="MDFDSESIAALAADMGDGLSGARLHGAMVGGLCGGGAEEGVWEP
                     VLEAALGDAQIGACEPELRQMLSLTEAALADPEYTFEPMLPDEGEPLADRVQALADWC
                     DAFVLAYAAAARDAERQQMSDEAGELLEDLTAIAGGLDPAAMGEDDEDEEDYMQILEF
                     VRIAALNLYAERRPGADVVLH"
     gene            14123..15469
                     /gene="pepP"
                     /locus_tag="PLW13_01995"
     CDS             14123..15469
                     /gene="pepP"
                     /locus_tag="PLW13_01995"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HQY69166.1"
                     /translation="MASAAAQPHPRIGPRDYARRRRELMALMGEGSIAIVPSARQLIR
                     NRDSSFPFRQDSDFHYLCGFDEPESVLVLAPGRRHGEFLLFCRERDRERENWDGLRAG
                     PEGACELYGADDAFPIGDLDDILPGLIEGRERLYYAMGKQPEFDHQLMSWINKIRAKA
                     RSGAHPPGEFLDLDHFLHELRLYKSAEELRVMQRAGAISAAAHVRAMRACKPGMFEYQ
                     LEAELLHEFMFHGARSPAYTSIVGGGANGCIMHYVENSAALRAGDLVLIDAGCEYEHY
                     AADVTRTFPVKGRFSAAQQALYEVVLEAQLAAIREVKPGQHWNQPHDVTVRVITAGLR
                     DLGLLDGEPEELIELAAYRTFYMHRAGHWLGLDVHDVGDYKIGGEWRVLEPGMVLTVE
                     PGIYIAPDNRAVAKKWRGIGIRIEDDVVVTKSACKVLTGEAPKSVQDIERLMAGGA"
     gene            15466..16701
                     /gene="ubiH"
                     /locus_tag="PLW13_02000"
                     /gene_synonym="visB"
     CDS             15466..16701
                     /gene="ubiH"
                     /locus_tag="PLW13_02000"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HQY69167.1"
                     /translation="MSDNSHDIVIVGGGMVGASLACALAAGGRFARVALVEGFPLLDR
                     AGPAVYQPSFDARSTALSLGSARFLARLGLWQDVRRHAQPIRRIHVSDRGRFGSTAMD
                     AAEEGLEALGYVVENQWLGNVLHDGLRACANLTLLAPARVDAVRRRVDRVIVRAKLAH
                     GGEERELDAGLAVIADGARSGLAAALGIVAQQQDYGQQALIANVAHRQPHEGRAFERF
                     TDAGPMAMLPLVDAPDASARSALVWVQEPDLAEQARTLPEPEFLRRLQQRFGYRLGRL
                     LRVGERHLYPLSLTRATEQARAGIVLLGNAAHALHPVAGQGFNLSLRDVAVLAQTLEE
                     ACVRGEAPGALAVLERFVARQRADQERTIGFSDALPRIFGSGLLPLAAARDLGLIGLD
                     LLPAARSLLVRHATGLAGE"
     gene            16728..17945
                     /locus_tag="PLW13_02005"
     CDS             16728..17945
                     /locus_tag="PLW13_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQY69168.1"
                     /translation="MGGRYDVVIVGAGIAGSALAAALAPAGLEILLLEAGALPEALPP
                     VARTIAAVDPRVSALGIASVRLLQEVGAWQRLPDGVASPYECMRVWEEDGSGRIGFDA
                     AGIAQPVLGHIVENRWVVATLLAALAEAPNVTVRGGERLLALEPVAGVDARQRLSLDS
                     GASVEAALVVGADGARSAVRELCAIEARQRDSGQQAIVATIETAAAHRRTAWQRFLQS
                     GPLALLPLATLAGGHACSIVWSADEPVAQALMQLDDAAFAARLTRASESCLGEVRAVS
                     RRFAFPLRPLHAAAYVAPGVALVGDAAHVIHPLAGQGINLGLSDVRVLAAEIVRARAR
                     GRAIGDAGVLARYQRRRRGENAVMLRAMEGFRILYGDDRPGARLARNIGMSAVHALGP
                     LKREFMRRAMGID"
     gene            18014..18403
                     /gene="gcvH"
                     /locus_tag="PLW13_02010"
     CDS             18014..18403
                     /gene="gcvH"
                     /locus_tag="PLW13_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQY69169.1"
                     /translation="MSNIPADRKYAVTHEWAQADASGIVTVGISDHAQIALGDVVFVE
                     LPELGAHIDAGDEAGVVESVKAASDIYAPVSGEVVEVNEALESSPELANSDPYGEGWF
                     FRLRADNTEELDALLDAEGYEHSLDEE"
     gene            18432..19634
                     /locus_tag="PLW13_02015"
     CDS             18432..19634
                     /locus_tag="PLW13_02015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HQY69170.1"
                     /translation="MSEAGGLPRLQLRRNEDRRIRQGHHWVYSNEIDTTATPLKQFAA
                     GEQVLLCTASGKPLGVAFVNPHSLICARLLERHAGEALGAQFLRERLRTALGLRERLF
                     SEPCYRLVYGDSDGLSGLIVDRYGEVLVVQVGTAGMERLETELIAALVELLSPAGILL
                     RNDASVREMEQLPLYTRVAFGEVPEYLELRENGASFRVSATGGQKTGWFYDHRMNRRR
                     VAELARGQRVLDVFSYVGGWGVQAAVAGARAVTCIDSSQPALELAAANAALNGVAERM
                     DFRAGNAFDELHALAQAGERYDIVVLDPPAFIKRRRDQKSGENAYHRLNREAMNLLAD
                     DAILVSASCSMHLGEAALIDIVRMAAFHAGRQLQILEVAGQGPDHPIHPAIPETRYLK
                     SVIARVHR"
     gene            19716..20207
                     /locus_tag="PLW13_02020"
     CDS             19716..20207
                     /locus_tag="PLW13_02020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HQY69171.1"
                     /translation="MIDEQGYRPNVGIILANSRGDLLWARRRGQNAWQFPQGGIHEGE
                     TPEQAMFRELGEEVGLEESDVEMLACTAGWLKYRLPPQFMRNRKNPNFVGQKQKWFLL
                     RLVSEDQRVRVDAHATPEFDQWRWVSYWYPLGQIVAFKREVYRRALWELAPRHQRLVR
                     NGA"
     gene            20227..22485
                     /gene="ptsP"
                     /locus_tag="PLW13_02025"
     CDS             20227..22485
                     /gene="ptsP"
                     /locus_tag="PLW13_02025"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQY69172.1"
                     /translation="MLKSLRRIVQEVVSAPDLPETLAIIVRRVRDVMRTEVCSVYIHD
                     PVSDRYVFMATEGLNKALEGRFSLALGEGLIGYVAKREEPLNLDNAEAHPKFKYIEGS
                     GEEVFKSFLGVPIINHRQVLGVLVVQQREPRRFNEDEEAFLVTMSAQLAGVIAHAGAV
                     GSYRGKPRDPSRRATEFPGVPGSPGIAVGRVVVVSPPSDLRSVPHRPCADPEAEVGLF
                     QDAVQAVREEVLAIREELSGRVRPEELTLFDAYVMLLDDHALSGEVIECIRGGVWAQG
                     ALSQVVLRHVRTFEMMEDAYIRERATDLRDLGRRILAQLQAGSRGRLEYPERTVLVGE
                     EIPASILAEVPEARLAGIVSVSGSGNSHAAILARAMGVPTVMGVVDLPFAEIDGAEIV
                     VDGHDGRVLVDPAPELRQQYLEAMEAERADARQMESCIDLPCVMTDGHAVRLLVNTGL
                     NSELMRTLGRGAEGVGLYRTEIPFLMRERFPTEEEQRLIYREQLEAFAPRPVTMRTLD
                     VGGDKSLPYFPIKEENPFLGWRGIRVTLDHPEIFLAQVRAMLKASAGLDNLRIMLPMI
                     SNTLELDDALALLSRARAELCEEGWTIATPQVGVMVEVPAAVYQAGDLARRVDFLSVG
                     SNDLTQYLLAVDRNNARVAALYHAYHPAVLQALQDVVRAAHDVGRPVSICGELAGDPG
                     AALLLVAMGYDMLSMSATNLARVKATLRRFARADAAQLLAEVMRMDTSRRIRAAVEEC
                     LRRGGIAVRPRG"
     gene            22619..23164
                     /locus_tag="PLW13_02030"
     CDS             22619..23164
                     /locus_tag="PLW13_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69173.1"
                     /translation="MGPKKVRLGPCALMMVALGGSMGAAGTSLLPPFSHIPVASETFE
                     ITVPEPFGLTQIRLEFEPGSSKRISVIEISYAGETYRIDGGILGEYPVVDETDFYYND
                     SDFQNDELDSFYFHVNHGAAIKVECGVDRIEHVRKMKIVLVRWHAEPIVADDESYFET
                     CARYTEAQKQFEEINGPIELD"
     gene            23201..23545
                     /locus_tag="PLW13_02035"
     CDS             23201..23545
                     /locus_tag="PLW13_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69174.1"
                     /translation="MYEAKHEPLLPRSAFLRRIALHVGATAAILAFSLAIGMTGYVVC
                     ERMTWIDAFLNTTMLLGGMGPVNPPLSAGGKLFAGLFALYAGLVFVVSTALVLTPVFH
                     RVLHCFHVSEED"
     gene            23550..24575
                     /locus_tag="PLW13_02040"
     CDS             23550..24575
                     /locus_tag="PLW13_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQY69175.1"
                     /translation="MRAAVFHETGRPLSIETVPDPRPAPDQVIIEVARSGICGSDLHV
                     TEYGAAPDGTILGHEFAGTIAEVGAAVRGDWKVGERVTALPITSCQQCEACTGGLPGL
                     CPQNIFTGTTLLVQGAYAQYVAARAGMLQRLPAGVPFAQGAMVEPLAVSHHAVEMAAL
                     RPGASVLVIGAGPIGAGVVLFARLAGARHIVVSERFSARRERALALGATAVIDPSAED
                     VAGRFAAHAGCRPQVVFECVGNPGLLRHAVEVAGIRARIVVAGVCLLDDTFNPLTGLL
                     KEVTIQFSQCYTEHNFAAVIDAMARGEAKPEPMHTGTVGFAELPAVFESLRAASPHCK
                     VLIDPDR"
     gene            complement(24600..25382)
                     /locus_tag="PLW13_02045"
     CDS             complement(24600..25382)
                     /locus_tag="PLW13_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HQY69176.1"
                     /translation="MCLIALAWQRHPEFPLLIAANRDEFHVRPTRAADFWPDHPGLCA
                     GRDLAAGGTWLGITRGGRFAAITNRRGHPAPPAAPSRGLLTLDFLVRSEPPREYMNRL
                     RASCEDYAGFNLLAGELDGDLYYLGNHGPRMERLEPGVYGLSNDMLDTPWPKLLGVRE
                     ELSALLCTHPEPEAILALLADRSVPPDHALPDTGVGLEQERRLGPRFICSPDYGTRTS
                     ACVRVHRDGWVEFTERSFDASGTAQQTLEYRFDIQESTPRPS"
     gene            25496..26305
                     /gene="lgt"
                     /locus_tag="PLW13_02050"
     CDS             25496..26305
                     /gene="lgt"
                     /locus_tag="PLW13_02050"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQY69177.1"
                     /translation="MLHHPNFDPVAVQLGPLKVHWYGLTYLAAFVAAWWLARLRARRP
                     GAPLTPEQVEDLIFWGAIGVVVGGRLGYVLFYGFDHLLADPTWLLRVWEGGMAFHGGL
                     LGVIAVMWWFARKHGQHIGDIFDFVAPIVPLGLGFGRIGNFIGQELWGRPTDLPWGMV
                     FPRDPLALARHPSQLYQAALEGLVLFLILWWYSSRPRPRFAVSGLFLLCYGLFRFAVE
                     FAREPDAGIGFVAFGWMTRGQLLCLPMIVAGLVMFVWAQRRAGGATPAATA"
     gene            26319..27113
                     /locus_tag="PLW13_02055"
     CDS             26319..27113
                     /locus_tag="PLW13_02055"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQY69178.1"
                     /translation="MHQYQQLLRHILESGRDKGDRTGTGTRSVFGYQMRFDLGAGFPL
                     LTTKKLHLRSIIHELLWFLRGDTNIRYLKEHGVSIWDEWADAEGELGPVYGYQWRSWP
                     AADGRHIDQIAQLLEQIRTNPDSRRLIVSAWNVADVDRMALAPCHALFQFYVQDGRLS
                     CQLYQRSADVFIGVPFNIASYALLTLMVAQVTGLAPGEFVHTFGDAHLYSNHLGQARL
                     QLEREPLALPVMALNPAVKDLFAFRFEDFELRGYQSHPHIPAPIAV"
     gene            27110..27622
                     /locus_tag="PLW13_02060"
     CDS             27110..27622
                     /locus_tag="PLW13_02060"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQY69179.1"
                     /translation="MKITLVVAMAQNRVIGRDNALPWHLPEDLKHFRALTMGKPIVMG
                     RRTFASIGKALPGRTSIVVSRQPDLDLPEGVLLAASVPDALVLAHEIAARDGVEECMV
                     IGGADIYRQCLPLAERIQLTLIEQEVEGDTVFPEFAHTDWRETARERHASAADPALRF
                     SFLTLDRVHP"
     gene            27619..28407
                     /locus_tag="PLW13_02065"
     CDS             27619..28407
                     /locus_tag="PLW13_02065"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HQY69180.1"
                     /translation="MKRYTAKWGALGAAVSLAAWLGATAARAEQPLEDIYRLSQASTQ
                     AAQASQATVERIADETRELLQEYKTVMKQVDGLEVYNARLEKQIAGQEERIAATGASI
                     DEVTVLQRQMMPLLVRMIDGLESFVALDKPFLLEERRERIAFLRHNLDRPDIALAEKY
                     RQVMEAYRIEIEYGRKIDTYRDTIAIDGEQREVDILRVGRIALLYQTPDRQAAGFWNP
                     AQGGWEPLEGSEYRGAIQEGMRMANKQASIELLKLPIPAPEAAR"
     gene            28404..29795
                     /locus_tag="PLW13_02070"
     CDS             28404..29795
                     /locus_tag="PLW13_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY69181.1"
                     /translation="MKALGRIITAWLGAGAIALGAAAEERQPQSLQQLLEFVQQGRAV
                     DAREAREREARFVADKAGRQAELERARALRAEQQKRSAALEKRFEANERELEKAQARL
                     RERLGSLGDLLGNVTAAAGDARATFQASLTGAELGGARVAKADELVRLAGSGVDLPTI
                     EHLEALWYELQQEMVASGTVTAFAAPVIRPDGNRAETPVVRVGVFNIVSAEGKYLDYQ
                     GDKGLFTELPRQPGGSFVDAAAGLAAATGGVHPFGIDPTGPSGGSYLAALIETPTFGE
                     RIRQGGLVGYITMALGLFGLILAAYRLVVLARMDARVAAQLASPRANPDNPLGRVLAV
                     HESNPHMDIETLELKLSEAVIRELPRIESGLTLLKIIATVAPLLGLLGTVTGMIVTFQ
                     AITIFGAGDPKAMANGISQALVTTVQGLCVAIPIVLAHTLVGNRARKITQILEEETTG
                     VIAEHTGYAQGKD"
     gene            29795..30319
                     /locus_tag="PLW13_02075"
     CDS             29795..30319
                     /locus_tag="PLW13_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY69182.1"
                     /translation="MSGLQEAMRGILDFVDAGGPVLYAVAGLTFVMWTLVFERAWYLR
                     VGLPADVRAMLAAWESRPERRSWHARQVRRALISRLGRSIGRNLALIRACVSLCPLLG
                     LLGTVTGMVAVFHVLAVSGGDTRSMAEGVSRATIPTMAGMVAAISGVFANLYVTRVAA
                     RELLLAEDRLTTDH"
     gene            30329..30742
                     /locus_tag="PLW13_02080"
     CDS             30329..30742
                     /locus_tag="PLW13_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY69183.1"
                     /translation="MRRNRVFSREERSAPGQIDLTPMLDVVFIMLIFFIVTASFIEEA
                     GIEVERPEAVTAQQKKSTRILVAVSADDQVWIAGHRADPRAVRREIERQLAASPHGGL
                     VVQADARSSAKAVAAVLDAARAAGIADVSLSTRRH"
     gene            30747..31352
                     /locus_tag="PLW13_02085"
     CDS             30747..31352
                     /locus_tag="PLW13_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY69184.1"
                     /translation="MYRWRYLFAAALGGAVTLALLALMHALIATGERALEESTPIRIA
                     DVTMPDARIESGFVARLPERIEEPERPPPAAALPEDFAAAGALVPDMKAALAPQLQIG
                     TGPGAVATDGEYLPIVKIAAVYPRRAQERGITGHCIVEYTVTTSGATRDPVPVDCSPP
                     GVFEESSVKAALRFKYRPRVVDGQPVEVSGVRNEFLYELDE"
     gene            31349..32704
                     /locus_tag="PLW13_02090"
     CDS             31349..32704
                     /locus_tag="PLW13_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HQY69185.1"
                     /translation="MNGALQGRQRGAIRRGLSRLGVAAALLALPALLAPAGWPGASSA
                     RAEDARAGQAQGTRRTPTLRNEVHAKLADVQKAAEAGDFATAERLLAALQAGFSGKRA
                     LNSYELANVHNFYAYIYYSQQKHPEAIRAYEKVLAQPGLPGAMETGTRYSLAQLYVAT
                     ADWKRAAAMLESWFRIAEEPAPEAWMLLAQTNYQIGEYDRALGNIDKGMAEARRRGMA
                     PKENWYLLARMVSYDKGDLRRTASVLEALARQWPRKDYFVQLSAIHGELKDEPRQLAA
                     MEVAYLAGWLGEERELVNMAYLYLGADMPWKAARVLEQGMEAGKVEAGAKNLELIGIA
                     LRQARENRRAIVYLERAAALAGDAEMWARLANLQLDEDASEQAVEAARKALAMGGGRR
                     PDHTQVVLGMALYNLGRYDEARAAFTEAGHDPRSEQVATQWLHFLQTEIARAAQLAQE
                     L"
     gene            complement(32717..34561)
                     /gene="ilvD"
                     /locus_tag="PLW13_02095"
     CDS             complement(32717..34561)
                     /gene="ilvD"
                     /locus_tag="PLW13_02095"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQY69186.1"
                     /translation="MPKYRSKTTTEGRNMAGARSLWRATGMKDEDFSKPIIAVANSFT
                     QFVPGHVHLKDMGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKTKLSEHKL
                     DLVDAMVIAVDDAAADETVAEYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGS
                     TLATHADRERLFLEAGRLVVALAKRWYGQDDASALPRSIASFRAFENAMTLDIAMGGS
                     TNTILHLLAAAREAEVDFTMADIDRLSRRVPQLCKVAPSTPKYHMEDVHRAGGIMSIL
                     GELERAGLLHADVPTVHSKTLHEALERWDVVRTQDATVHEFFRAGPAGIPTQTAFSQS
                     TRWDTLDLDRAEGCIRNAEHAYAQEGGLAVLHGNIARDGCVVKTAGVDESIWVFEGPA
                     HICESQDEAVEHILHGLVKEGDVVIVRYEGPRGGPGMQEMLYPTSYLKSKGLGKACAL
                     LTDGRFSGGTSGLSIGHVSPEAAAGGAIALIENGDRIRIDIPGRTIEALVSDEELARR
                     RAAMEAKGAAAWKPARARPRKVSAALRAYAMLCTSADKGAVRDLDKIL"
BASE COUNT         5095 a        11990 c        12543 g         5095 t
ORIGIN      
        1 tgaatctgga gaccctgcct gcatttctga acgccggggc gtgtgccgtc ggccgtgccg
       61 tccagcgtca gcgtgttgcc gtcgacatcg aagcgcgccg ccggtgtggc gctggagcgc
      121 aggtccgcgc ccccgcgggg gcggccgtcg gcatggcgcg acaccgcacg gtcgcggctc
      181 cacaacgggt cctgctcgaa gaccgtggcc gcgaacagca gcgatccgcg cgaccaggtg
      241 ttgcccgcga gcaggctggt gctgcgcgtg gcgcggtcgc cgcgctgcgt gctgccgtac
      301 tgcgtgccga gctgcacgcc gtcatagccg ctcttcatga tgatgttcac cacgcccgcg
      361 atggcatccg agccgtacac cgccgaggcg ccttccttca atatctcgat gcgctccacc
      421 gcggcggggg ccagcgtgtt gaggtcgaag gcgtccccgg ccaggccgcc ggcgctggag
      481 cgccggccgt tgaccagcac cagcgtgttg cccgcgggca gcccgcgcag cgtcacggtc
      541 gcggtgccgt cgccgccgtt gctgaccgcg gtgctggtct ggttgccgat caccgcgggc
      601 atgaagcgca gcaggtcgcc gaggctctgc gcgccgctcg cctcgatctc cggggcgccc
      661 agcacgtgca cgggccccga gccgaggtag ttcgcctgct tgatgtgcga gccggtgcgc
      721 agctgttcgc gcacgatgat ctgttcgatc acgcgggcgt ccacgtaacg ctccgcctcc
      781 gtgccgggtt gcgcagccgc gggcgcgcaa tccgccgcgc tgttgcaggg cggtccgatc
      841 gagaccacgt tgtcaccgag gtaggccggc accaggccgc tgccctcgag cagccgctcc
      901 agcgcctggc gcgggctgag cgtgccggtg atcgcgacgg tggggttgct cggcacgttg
      961 gcggtcgcga acaccagcga cagctgcgcc tggcgggcca gttcgatcag cgccgcgggc
     1021 agggtgctcg ccgggacggc gaactgcacg cgggcctcgg tgtcggccag gacggccgtg
     1081 gccagggaaa tgcccagcaa caggcccagc gcgcgccgtc gcgggccacg tcgtgcaggc
     1141 tggtgatgag aaccgctccc cggcatctgt ttccgtcgcc aacgcaatat ccgcccggaa
     1201 tgggggatgc cgccagtggc cgaacgataa caaaacaggc cggggatggc gcgtcggatt
     1261 gccgggctct cagtccgtgc ggcgggagat cagcagcgga ccctgttcgg ggccggtcac
     1321 ggcgaggccc aggctctgtt ccagcgccgc gagggtgagc cggggctcgg cggtactgaa
     1381 cacgccggag acgcggatcg tgccggccgc gggatcggcg aggcgcaggg cgggctccat
     1441 gtagcgctgc agttcgccga tcacctcggc gagcgggacg tcctcgaaca cgaaccggcg
     1501 ctgctgccag gcgagcgcct tgtccgcgtc gacccggctg atctcggcga ggttgtcctg
     1561 gcggtcgtag cgcacggcat cgccggcgcc gagctcgcgc gaggcgccgg cgccgacgcg
     1621 cgtcgcgaag cgcacgcgcc cgtgggtcac caccacggac atgcccccgc tgtcgcaggt
     1681 ggcgccgaag gcggtgccca gcaccgtgac gctggcctcg ccgcataccg cgctgaaggg
     1741 tcgcgcggca tcgggtgcca cgctgaagaa gacctcgccg tgatccatgg tcgcggtacg
     1801 cgtctcgtcg cgcagcgcca cgctgagcgc ggtgtcggtg ttcagcgtga tctcgctgcc
     1861 gtcggcgagc cgcagctgct ggaatccgcc caccggggtg cgcagttcca gcgccggcgg
     1921 ctcggcaccg cgcggcagga ccagcagcgc cgccaccgcg gccgcggcgg caaggccgag
     1981 cggcagctgc cagcgccgcg cgcgcgcggt gcgggggatc tcgatcggca gcgcgcgcag
     2041 cacgccgaga tccgaccaca gctccagcat gcggtcgaag gcggccgcgt tgccggcatc
     2101 ctcgctcagc cagcaggcga accgcgcgcg gtccccggcg ctgacgtcgt ccgcgcgcag
     2161 gcgcgcgatc caggcggcgg cctggtcgag cgcttggtgc tgtgctggcg tcgtgcgggt
     2221 cactgcggtt ttccactccg gatcctgccg gatcatcgcg tgcgtacgtg tcttgtgctt
     2281 cttctcacag cctaagacga acgaaagccg aaatacctcc gccattcggg ggaagaaatt
     2341 cgcacattcg tcgggggtgc catgcgggcc agtgccggca ccggtaccgg caggctcagg
     2401 ggatcaggtg ttcgcggcag tgcttgagcg cttcgaggat gtatttctcc acgctgctga
     2461 ccgacacccc catgtcccgg gcgatctcgg cgtaggacag tccgcgcacg cggtgcagca
     2521 tcagcgcctg gcggcagcgc agcggcaggt cctcgatggc gcggtagatc agcccgagct
     2581 gctggcgtgc agccaccagc tcttccggcg tgcccggatc ctgctgcgcg gtgtcgtcct
     2641 gcagccgagc gccctcttcc tccaggtagc gcgcgtgcag cttgcgccgg cgcagctggt
     2701 cgatggccat gttcgaggcg acctggaaca gataggcgcg ggcgttctcg aggtcgtcgg
     2761 catgctcgag ccggtgcagg cgcatgaagg cgtcctgggc gatttcctgg gcgtcgtcga
     2821 tgctgcccag cttgcggctc aggaagcgca ccaatgcgct gcgttggctg ctgatcagcg
     2881 tctccagcag ctcgccgggg cgtttggcca tgatgtccct ggtgtcgatg gtcctgctct
     2941 cctccgggct gtcgggtggg ggcatcgcgc aagcggagcg aggccgcgaa ctcctgactg
     3001 ccggcacggt ggcgcttctg tcgggcgttt acatggtccg tgggtagcgg gtgaccggtt
     3061 ctgcgccggt ctgacctgag gttagcacag cactccccgc tctccgggag gatttgtacc
     3121 gcataaacgc gccgcatccc ccgaacgggg tataccgccg cgccgtcccg ggcggatcag
     3181 gagagcaggt ggcaggcggc cagtcgtccc tcggcaacgg ggcgcaactg cggcgcctcg
     3241 ccgtggcagc gcccggtggc gagcgggcag cgcgtcgcga agccgcagcc cggtacggcg
     3301 tcgagcggcg agggcggctc gccgctggcc ggcacgtgcg cgcgggcccg tgcggcgcgc
     3361 ggatcgggtt ccgggttcgc ggccaccagc agttgcgtgt aggggtgcag cggcgcggac
     3421 cagagcgcat ccgcggggcc ttcctcgacc agccggccga ggtacatcac cgcgatgcgc
     3481 tgcgagaggt ggcgcaccat cgcgaggtcg tgcgcgatga acagcagcgt gagccccagg
     3541 ctcgcgcgca gctcctccag caggttcacg atctgtgcct gcaccgagac gtccagcgcc
     3601 gagatcggct cgtcgcagac cacgaactcc ggcccgggga tcagcgcgcg cgcgatgccg
     3661 atgcgctggc gctggccacc ggagaattcg tggggatagc ggcccatgtg gccggcggtg
     3721 agcccgacgc gctccagcca gtgcgccgcg agctcgcggt gctcgcgccg cgcgccgccg
     3781 tcctgcaggc gcaacggttc gcacaggatc tctcccaccg tcatgcgcgg gttcagcgag
     3841 gcgtagggat cctggaagat catctggatc gcgcgtgcct ggcggcggaa atcggcgggg
     3901 cggtggcgcc gcggcagcgg ccggccggcg tagagtatct cgccggcgct gggcgcgagc
     3961 agtcccacca gcgtcttgcc cagcgtggac ttgcccgagc cgctctcccc gaccagcccg
     4021 gtaacggtgt cgcgcgcgat cgtgagcgag acgccgtcga ccgcgcgcac ggtctggccg
     4081 cgcgcggtgc gaaaatgcgt gtgcagcgcg cggatatcga gcagcggcgt catcgtgcgc
     4141 tcccgtccgc ggccgcgcgg tggtgcagcc agcagcgcgc gccgtgaccg gcagcgagcg
     4201 cgaacccggg cggctcgagc cggtcgcaga tccgcatcgc gcgcgggcag cgcgcggcgt
     4261 aggcgcagcc cggcgggggc gcgcgcaggt cgggcgggct gcccgcgatc ggttgcagcg
     4321 gctgcccgcg cgcgcgcgcc gcgccgggca gcgcctgcag cagcccggcg gtgtaggggt
     4381 gggcatggcg gtaaaagatc tcgtccaccg gcccgtgctc caccacctgc ccggcgtaca
     4441 tcacggccac gtcgtcggcc atgcgcgcga ccacgccgag atcgtgcgtg accagcacga
     4501 tggccatgcc gtcctcgcgc tggatctcct gcatcaggcc gagcacctgg tcctgcaccg
     4561 tgacgtccag cgccgtggtc ggctcgtcgg cgatcagcag cgccggcctg caggcgagcg
     4621 cgatcgcgat catcacgcgc tgcagcatgc cgcccgaaaa ctcgaagggg tactggcgcg
     4681 cgcgctcgcg cgccgccggg atgcgcgtgc gctcgagcag ctccacggca cgcgcgagcg
     4741 ccgcgcggcg gctcatgccg cggtgcgcca ccagcggctc ggcgatctgc tcgcccacgc
     4801 gcatcagcgg gttcagcgcg ctcatcggat cctggaagat catgccgacc gtcgcaccgc
     4861 gcaggcgatc gagctcgcgc gccggcagcc cgagcagctc gcgcccgagc agccgcgccg
     4921 agccgcccgt gatgcgcccg ggcggtgtgt cgatcagccc catcacggcc tgcagcgtca
     4981 ccgacttgcc gcagccggac tcgcccacca cggcgagcgt gcgccccgca gcgaccgcgt
     5041 agctcgcgcc gcgcaccgcg cgcaccgtgc ccgcgcgcgt ggcgaactcc acctccagtc
     5101 cgtcgaccgc cagcaccggc accgcctccg cgctcatgcc ggctccgcgc gcgtgtcgag
     5161 cgcgtcgcgc agcccgtcgc cgagcaggtt gaaggccagc accgtgatgc tgatgaacgc
     5221 ggccggtacc gccagctcgt gcggatgcgc cagcatgctg cggatgccgt cgttgcacag
     5281 cgagccccag gacggtgtcg gcggcgcgac gccgagcccg atgaacgaca ggaatgcctc
     5341 ggtgaagatc gcgctcggga tcgcgaacga cagcgccacc agcagcacgc ccagcacgtt
     5401 cggcaccatg tggcgcagga ccaggtacgg agtgccggcg cccatcaggc gcgccgcctg
     5461 cacgtagggc tcgtcgcgga tcgccatcac ctgcccgcgc accagccgcg ccgaggcggg
     5521 ccagccgagc agcaccatcg ccagcagcat cggcatcacg ccgctctcgc cggggcggat
     5581 gccgaaggcc accttgaaca ggatcatgaa cagcaggaag ggcagcgcaa ccacgaagtc
     5641 cgccagccgc atcatcgcgt tgtcgatgcg cccgccgagg tagcccgcca gcccgccgta
     5701 gagcaggccg gccagcacga acagcagcgg cgccgcgatg ccgatgaaca gcgaggtgcg
     5761 ggcgccgtgg atgatgcgcg cgaggatgtc gcgcccgagg taatcggtgc cgagcgggtg
     5821 cgcgggcaac cgcagctcgc cgccgatgcg ttcgtggccg gcgttggccg ccagcagccc
     5881 gcgccgcagc gccgccgacc acgcgatggc ttgccgcggt tcgacgcgca gcgtgatcgc
     5941 cgcgccggcc tcgagccccg cgcgcagcgg taccaccgag taatggtagt tgcgcgcttc
     6001 cagcccgagc cggtcctcga aggccggagt cacgacctcg ccgagcggca cgccgaggtc
     6061 ctcgcggtcg tgcggcgcga gctcgtggcg atacaccgcg taggcatcgg cccccgcgac
     6121 aggatgccag gccaggcgca ccgccgcggt gtgcggcgtg ccgcagaccg tgaggccggc
     6181 cggcgtctcg ggtgcgcacg gcaccgccac cggtcccggg tccgcgacca gcagcgcggc
     6241 gcgtccaggg cccggcggcc gcgaggcctc gtcgagacgc tgtagcgcgg ggtcaacgcg
     6301 ccacagccac ggcccggcca gagcgccgat gatcagcgtc gcgatcacgg cgagcgagag
     6361 catcgcacgg cggtcggcgc gcagccgcgc ccagacgtgc tcgcggtacg acggcgacgg
     6421 cgtcgcggcg aggggttgcg ccacgccggc ggcgagcggc aggaaatccc ccggcagcgg
     6481 gacgtggctg ctcatcgcgc gcggctccgg gtcgggtgca tcagcgcgcc tgcgccatgc
     6541 ggatgcgcgg gtccaccagc ccgtaggcaa tgtccacgag gatcaccatg aacaccagga
     6601 aggcgccata gaacaccgtg gtgcccatga tcacggtgta gtcgagctgc tgcacggcct
     6661 gcacgaagta gcgcccgagg ccggggatcg cgaacaccag ttccaccacg aaaccgccgg
     6721 tggcgatggt cgctatcgcg gggcccagca ccgtgatcac cggcaggatg gcgttgggca
     6781 gctggtggcg cagcaggatg cgcgccggcg agaggccctt ggcacgcgcg gtgcgcacgt
     6841 aggcggcgcc gcgcacctcg agcatcgagg tgcgcatcag ccgcgtcagg aaggccatgg
     6901 tgccgaggcc cagcaccagc gcgggcacca gcatgtgcgc gggtccgccc catccggcca
     6961 ccggcagcag cgacacgccg gcccaggcgt tcaggcgcag caccccgagc tgcgccagcg
     7021 cggcgagcac gaaacccggc accgagatgc tcagcaccac caggaacatc acgagcgcgt
     7081 cgggccagcg ccccgcggtg cgtgccgtga tcacgcccca gaacaccccg cccagcgcgg
     7141 cgaagccgat ggccagcacg cccagcaacg ccgagaccgg gaagtgctcg cggatgatgt
     7201 cgttgacctc gcggttctgc tgcgtgaacg agacgccgaa atcgccgcgc gccatgttgc
     7261 cgaggtagcg caggtactgc agcgccagcg gctggtcgag gccgtagcgc gcctcgaggt
     7321 tgcggcggat ctcgggcgac acggcgcgcg catcgagcag cggatcgccg ggcacggcgt
     7381 gcatgccggc gaaggtcgcg gtcgcgatga accagacggt gagcagcccg tagcagaggc
     7441 gtccgaggat gaagcgcagc atcggccatt gatagcggca ttgcccgcgg ctgtaaagca
     7501 aggcacggcg cggtgataat ggccgcgcca gtccggccgc cggttgcggg gtgttcccat
     7561 gaagcatctg atcatcgtcc gtcacgccaa gtcgagctgg gccgatccgg gccagcgcga
     7621 tttcgaccgc ccgctgaacg ggcgcggcga gcgcaacgcc cccgagatgg ccgggcgcct
     7681 gaaggcgcgc gggctgcgcc cgcagctgat cgtcgcgagc ggggcgcgcc gcgcgcacgc
     7741 caccgcgaag ctgatggccg aggtcttcgg ttatgcgaag gcggacatcg aaatcgtcga
     7801 cgcgctgtac gaggccagcc cgcgcgtgtg gctggagcag atcggcgcgc tgcccgccgg
     7861 cctcgacacg gtgatgatgg tcgggcacaa ccccgagatc accgcgctgg tcgcgcaact
     7921 gtgtccacag gcgcgtatcg acaacgtgcc cacctgcggc gtgctgtgcc tggactacga
     7981 ggccggcgac tgggccggca tcgcccgtgc aaagcccgtg agctgggaat tcgattaccc
     8041 gaagaagccc tgagctctgc taccatcaat gccccagcgc aaccaggcca gcccatgttg
     8101 cacccagtcc tcgaacgcga acgtcgcacc gtcgccgcgt atctcagcgc cggcgcccac
     8161 cggttgcagt ccctgttgcc gcgggtcgag cacgacgccg acatcgaggc gctgcaccag
     8221 tttcgcgtcg agctgcgacg catccgcacg gggctcatgg cccagcgcgg agcgctgccg
     8281 ctggccgatg ccgcggagct ggtcgcggag tgtcgctggc tcgcggggcg cggcagcgga
     8341 ctgcgcgatc tcgacgtctt ccagcaccgg ctgacggagt atctggagcc ggacatgggg
     8401 gcggatacac agccgttgcg tcgcctgcgt gccgacctgg ggcgactgcg cagcagcgcg
     8461 cggcgccagc tgctgggctc gctgcgcagt cgtcgcgccc atgcgctggt cacgcgactg
     8521 caggcgctcg gcgagctgcc ggcggccgca cccggatggc ccggcctgcc cacggccggg
     8581 gcggcgctgt ggcgcagcta ccgtcgcgtg cgcaggctcg gcaaggccat cgacgcgacg
     8641 tcgccccccg agcacctgca tgagctgcgc aagcgctgca agcgcctgcg ctaccagctg
     8701 gaaatgtatg cgggtgcctt cgacgaggcc gagctgcccg acatggtgcg gcgcctgcgc
     8761 aagctgcaga acgtgctcgg cgactaccag gacttccaca cgcacgccgc gctgctgtgc
     8821 gagctgcgcg cccgcgcggt ggattccggc gcaccggatg ccgcgtatct cgcgctgatc
     8881 gagcgcctcc tcgtggcgct ggacgaacgc tccgcggcgg cgcgggcgcg tttcgccgcc
     8941 cgcttcgcgc agttcaacga ccgcaagcac caccagcgcc ggcgccggct gttcgcgccg
     9001 gacccgcggc tggcgcgacc gatgatcggc agcggcggct actgccacgc ccgggtgtcc
     9061 ggcgagcgca tcggcctgcc cgtgggcaag gtggtgtgcg tcgggcgcaa ctacgccgcg
     9121 cacgccgccg agctaggcaa tgcggtgccc gaggtgccgc tgctgttcat caagccgccc
     9181 tcggcggtgg tcgacatggc gccgcagatc cgcatcccgg gcgggtgtgg cgcggtgcac
     9241 cacgagctgg agatcgcgct gctgatcggg cgcgagttgc gcgcggcaac gcccgaggag
     9301 gcctgggccg ggattgccgg cgtgggtctc gcgatcgatc tcacgctgcg cgagcgccag
     9361 gatcaattga aggcgaagag ccatccctgg gaaatcgcca agggtttcga cggtgcctgc
     9421 ccgctgtcgc ccttcgtgcc gctcgatccg gcgctggacc tggcggcgct ggaaacgcgg
     9481 ctggtggtca acgggcgccg gcgccagcac ggcaacagcg cgcagatgct gacgccgatc
     9541 atcgagctgc tgtgctacgc gagccgccag ttcagcctgt ggcccggcga cgtggtgctg
     9601 accggcaccc ccgcgggcgt gggcccgctg gcgcccggcg accgcatcac ggccgagctg
     9661 aaggggctcg tggtggtgca cgcggaaatc gtgtaaacaa tcacgcgcgc agctccctta
     9721 cgccgtttgc gcccgccagc agcaacaggt cagcggggcg gtgcgcgaat atgccgttgg
     9781 tcacgacgcc cacgatgttg ttgatcgccg cctccgtctt cagtggctcg ggcagcgcga
     9841 aatcatagac gtcgaggatg atgttgccgt tgtcggtggt gacaccctgg cggtagaccg
     9901 ggttgccgcc cagcttcacc agctcgcgcg ccacgtggct gcgcgccatc gggatcacct
     9961 cgaccgggag cgggaagccg cccagcaccg gcacgaactt gctctcgtcg gcgatgcaga
    10021 cgaattcgcg cgccaccgcc gcgacgatct tctcgcgcgt cagcgccgcg ccgccaccct
    10081 tgatcaactg cagcgcggca ttggcctcgt cggcgccatc gacgtagatg tcgatgccat
    10141 cgaccacgtt caggtcgaac accggcaggc cgtggccgcg cagccgggcg gccgaggcct
    10201 ccgagctcgc gacagtggcc gtgacctgcg cgcgcacgcc cgcgagcagg tcgatgaagc
    10261 ggttcgccgt cgacccggtg ccgatgccga tcacgctgcg ctcgtcgagg cgctcgcgca
    10321 cgtaggcgaa cgccgcggcg gcggccgcct gtttcagttc atcctggctt ttcatcgcgg
    10381 gcgcaaccca agcttgccgt ggggcggcaa gcttaagtga tcgcgccggc cgaggtacag
    10441 cgcgagccgt catagtggca gggctttggc tggcgcggca ccggcgcgct cattaccatg
    10501 ggttctcgcc aattttccgg gacactgtcc gcgccatgcc gaagtcctac atcaagcgca
    10561 tcctcgaggc gcgcgtctac gatgtcgcca tcgagagccc catcgacgag gcgccgcagc
    10621 tctcggcgcg cttcggcaac cgtatcctgc tcaagcgcga agacctgcag ccggtgttct
    10681 cgttcaagct gcgcggtgcc tacaacaaga tcgtgggcct cagcgcgccc gagcgcgagc
    10741 gcggcgtgat cacggcgtcg gcgggcaacc acgcccaggg tgtcgcgctc tcggcgcagc
    10801 gcctgcagat caaggcgctg atcgtgatgc cgcgcaccac gcccgacatc aaggtcatgg
    10861 cggtgcgtcg gcgcggtgcg cgcgtggtgc tgcacggcga caccttcgac gaggcatcga
    10921 agcacgcata cgagctcgcc gaacagaagg ggatgagctt cgtgcacccc tacgacgacc
    10981 ccgaggtcat cgcgggacag ggcacgatcg gcatggagat cctgcgccag ctctcgggtc
    11041 ccatcgacgc ggtgtttgtg ccggtgggcg gcggcgggct gctcgcgggt gtcgcggcct
    11101 acatcaagta cgtgcgcccc tcggtgcgcg tgatcggcgt cgagcccgag gacgcggcct
    11161 gcctcgacgc ggcgctgaag gccaaacggc gggtggtgct ctcgcaggtg ggaatcttcg
    11221 ccgacggggt cgcggtggcg cagatcggca aggaaacctt ccgcatcatc cgccagaccg
    11281 tcgacgaggt gatcaccgcg agcaccgacg agatctgcgc ggcgatcaag gacatcttcg
    11341 acgacacgcg ctcgatcacc gagcccgccg gcgcgctcgc gctcgcgggg ctcaagaagt
    11401 acgtgcagcg caccggggtg cagggccaga cgctggtggc gatcgacagc ggcgcgaaca
    11461 ccaacttcga tcgcctgcgc cacatctccg agcgcaccga gatcggcgag cagcgcgagg
    11521 cggtgctcaa cgtcactatc ccggaaaagc ccggcagctt tcgcgcgttc tgcaacgcca
    11581 tcgggcgcgg gcgttcgatc accgagttca actaccgcta ttccgacgcc ggcgccgcgc
    11641 agatcttcgt cggcgtccag gtgtccccgg agcgcaacga ccgcgaggcg ctgctcgcgg
    11701 agctgcgcgg caagggctac gaggtgatgg acctcaccga caacgagatg gcgaagctgc
    11761 acgtgcgcta catggtcggc ggtcgcgcgc cctctatcgg cgacgccgag atcgtctacc
    11821 gcttcgagtt tcccgagcgt ccgggcgcgt tgctcaactt cctgaaccgc atggagcacg
    11881 actggaacat ctcgctgttc cactaccgca accacggtgc cgcctacgcg cgcgtgctcg
    11941 tgggcttcca ggtgccggag ggcgatcgtg gcgcgctggc ggagttcttc gccgagatga
    12001 cacgccgcca ccgcgtctac agctactggg acgagaccga caacccggtc tatcgcgcgt
    12061 tcctgcgctg aattcagcgc gcgcggcagt cgatcagtcc atcctcggtc atgacggccg
    12121 ccagcggcac gtcccaggct tcgcgcgcca gcgccggcag ttcctggcac gcgaagccga
    12181 tgcccaccag cagcggtgcc gccgccgcgc gccggagcag gaaatcgaag ctgcggtcat
    12241 agaagccgcc gccctgcccg aggcggttcc cggcgcggtc gaagcccagc agcggcacgc
    12301 acaccagccc gatggcgtcg gcggcgattt cctgcgccgt ctcgtgcacg ggttccggga
    12361 tgccatacca gttcgtgtgc agcgcgtcgc cctggcggta gcgcgcgaag cgcaaccgct
    12421 tgtcgggctg catcacgggc aggcagaact gcttgtgcgc gtggcgatcg tcggcgagca
    12481 gcgggcgcag atcgacctcc tcgcccaccg gccagtacag cgcgacgcgc gacgcggcgt
    12541 gcaatagcgg ggattcgccg aggtgccggc agatcgcctg tgaggcgcgc gcgcgcccgg
    12601 tggcgccgag cgcggcgcgc gcatcgcggc actggcgccg cagcgtgctg cgtgcggaca
    12661 tgcgaggcct ccggcggatg cgagggcgat cgaagcaagg atgagggatt ccccggcgct
    12721 accgctgccc gtgtcgccct tgaacccagc ggttcaaggc ggcggcctca gtgacgcatc
    12781 aggctttccg gcaggcggac atgcacacag cgtcggcgga gaggccccca ggcaagtgct
    12841 tatcggctcg aggactaaca ggctggcgaa tagtccaggg aatcggcggt cagtatatca
    12901 gcgcgtgccg ggacccggag gcgggaaaat ctacatttcg atctggcggt aattgttgag
    12961 cgtgtcgtcg atccgggcgc tcaggcggcg caggcggtcg tgtacatcct ggctgccctt
    13021 gtcggattgc tcgccgcgct ggatcagctc gtgcgtgatg ttgagcgcgg ccatgacggc
    13081 gatgcgctcc aggccgatca ccttgccgct ctggcggatc gcgcgcatct gctgatcgag
    13141 atagcgcgcg gactcgacca gcgcatcgcg ctgctccgcc gggcaggcca cctggtagtc
    13201 cttgtcgaga atgtgcacgc gcacggtctg cagttctgcg ctcatgattc ctgttccagc
    13261 gagcgcagcc gcacgatcat ggcctcgacc ttggagcgcg cgtcctcgtt cttgcgcagc
    13321 agttgcgcgc gttcgtcgcg caacatgcgc tcggcggcct tcaatgtctt gatctcctgg
    13381 tccatcctcg cgcagaggcg tatcaggtcg tcgacgcgtt gttcgagggc tttcaattgc
    13441 tggccttcca tgggatgcct tcatcgcatc gcgaggttcc ggtactatag tgaggccttc
    13501 gacgacggtc aattcgagcc gccgcgccac ggtcccgatc agcttcgtcc ctccggaaag
    13561 cgagtatgga tttcgattca gagagcatcg ccgcgctggc tgccgatatg ggagacgggc
    13621 tctcgggcgc gcgcctgcat ggcgcgatgg tcggggggct gtgcggcggc ggcgccgaag
    13681 agggcgtctg ggagcccgtg ctcgaggccg cgctcggcga tgcgcagatc ggtgcctgcg
    13741 agcccgagct gcgccagatg ctgtcgctca ccgaggccgc gctggcggat cccgaataca
    13801 ccttcgagcc gatgctgccc gacgagggcg agccgctcgc cgatcgcgtg caggcgctgg
    13861 cggactggtg cgacgccttc gtgctcgcct acgccgcggc cgcgcgcgat gcagagcgcc
    13921 agcagatgtc cgacgaggcg ggcgagctgc tcgaggacct caccgcgatc gccggcgggc
    13981 tcgatcccgc cgcgatgggc gaggacgacg aagacgagga agactacatg cagatactcg
    14041 aattcgtgcg catcgcggca ctcaacctgt atgcggagcg gcgccccggc gccgacgtgg
    14101 tgctgcactg agccgcgcgc gcatggcatc ggccgcggcg cagccgcatc cgcgcatcgg
    14161 gccgcgcgac tacgcgcgcc ggcgccgcga gttgatggcc ctgatgggcg agggcagcat
    14221 cgcgatcgtg ccctcggcgc gccagctgat ccgcaaccgc gactccagct ttccctttcg
    14281 tcaggacagc gatttccact atctgtgcgg cttcgacgag ccggagtcgg tgctggtgct
    14341 cgcgcccgga cgccgtcacg gcgagtttct gctgttctgc cgcgagcgtg accgcgagcg
    14401 cgagaactgg gacggcctgc gtgccggccc cgagggtgcc tgcgagctct acggcgccga
    14461 cgacgcgttc ccgatcggcg atctcgacga catcctgccg gggctgatcg agggccgcga
    14521 gcggctctac tacgcgatgg gcaagcagcc ggagttcgac caccagctga tgagctggat
    14581 caacaagatc cgcgccaagg cgcgcagcgg cgcgcatccg ccgggcgagt tcctcgacct
    14641 cgaccatttc ctgcacgagc tgcgcctgta caagagcgcc gaggagctgc gcgtgatgca
    14701 gcgcgccggc gcgatttcgg cggcggcgca cgtgcgcgcg atgcgcgcct gcaagcctgg
    14761 catgttcgag taccagctcg aggccgagct gctgcacgag ttcatgtttc acggcgcgcg
    14821 ctcgccggcc tacaccagca tcgtcggtgg cggcgccaac ggctgcatca tgcattacgt
    14881 cgagaacagc gcggcgctgc gcgccggcga cctggtgctg atcgatgccg gctgcgagta
    14941 cgagcactat gccgcggacg ttacccgcac cttcccggtg aaggggcgct tcagcgcggc
    15001 gcagcaggcg ctgtacgagg tggtgctgga ggcgcagctc gccgcgatcc gcgaagtgaa
    15061 gcccggccag cactggaacc agccgcatga cgtgacggtg cgcgtgatca ccgcggggct
    15121 gcgcgatctc ggcctgctcg acggcgagcc cgaggaactg atcgagctcg cggcctaccg
    15181 caccttctac atgcaccgcg ccggccattg gctcgggctc gatgtgcacg acgtgggcga
    15241 ctacaagatc ggcggcgagt ggcgcgtgct ggaaccgggc atggtgctga ccgtggagcc
    15301 gggcatctac atcgcgcccg acaatcgcgc ggtggcgaag aagtggcgcg gcatcggcat
    15361 ccgcatcgag gacgacgtgg tggtcacgaa gagcgcatgc aaggtgctca cgggcgaggc
    15421 gccgaaatcg gtgcaggaca tcgagcgcct gatggcgggc ggcgcatgag cgacaacagc
    15481 cacgatatcg tgatcgtcgg cggcggcatg gtcggcgcga gcctggcctg cgcgctcgcc
    15541 gcgggcgggc gcttcgcgcg cgtggcgctg gtcgagggtt tcccgctgct ggatcgcgcc
    15601 gggcccgcgg tgtaccagcc cagcttcgat gcgcgctcga cggcgctgtc gctgggcagc
    15661 gcgcgtttcc tggcgcggct cgggctgtgg caggacgtgc gccggcatgc gcaaccgata
    15721 cgccgcatcc acgtctccga ccgcgggcgc ttcggctcga cggcgatgga tgccgccgag
    15781 gaggggctcg aggcgctggg ctacgtggtc gagaaccagt ggctgggcaa cgtgctgcac
    15841 gacggcctgc gcgcctgcgc caacctgacg ctgctggcac cggcgcgcgt ggacgcggtg
    15901 cgccggcggg tcgaccgcgt gatcgtgcgc gcgaagctcg cgcacggtgg cgaggagcgc
    15961 gagctcgatg ccggcctcgc ggtgatcgcc gacggcgcgc gctccgggct ggccgcggcg
    16021 ctcggcatcg tggcgcagca acaggattac gggcagcagg cgctgatcgc caacgtcgcg
    16081 caccgccagc cgcacgaggg ccgggccttc gagcgcttca ccgacgccgg ccccatggcg
    16141 atgctgccgc tggtcgacgc gcccgacgcc agcgcgcgca gcgcgctggt gtgggtgcag
    16201 gagccggacc ttgccgagca ggcgcgcacc ctgcccgagc ccgagttcct gcgccgcctg
    16261 cagcagcgct tcggctaccg gctcggtcgc ctgctgcgcg tgggggaacg tcatctctac
    16321 ccgttgtcgc tcacgcgcgc cacggaacag gcgcgcgcgg gcatcgtgct gctcggcaac
    16381 gccgcgcacg cgctgcaccc cgtcgcgggg cagggattca atctctcgct gcgcgacgtg
    16441 gcggtgctcg cgcaaacgct ggaggaggcc tgcgtgcgcg gcgaggcgcc gggcgcgctc
    16501 gcggtactgg agcgcttcgt ggcgcgccag cgcgccgacc aggaacgcac catcggcttc
    16561 agcgacgcgc tgccgcgcat cttcggcagc ggcctgctgc cgctcgcggc ggcgcgcgat
    16621 ctcgggctga tcgggctgga tctgctgccg gcggcccgca gcctgctggt gcgccacgcc
    16681 acggggctcg cgggcgaatg aagacggcag gcggcaaggg gcgcggcatg ggcggacgat
    16741 acgacgtggt gatcgtgggc gcgggcatcg cgggctcggc gctcgcggcg gcgctcgcgc
    16801 cggcggggct cgagatcctg ctgctggagg caggcgcgct gcccgaggcg ctgccgccgg
    16861 ttgcccgcac catcgcggcg gtggatccgc gcgtgagcgc gctcggtatc gcctcggtgc
    16921 ggctgctgca ggaggtgggt gcctggcagc ggctgcccga cggcgtggcc agcccctatg
    16981 aatgcatgcg ggtctgggag gaagacggtt cggggcgtat aggcttcgat gccgccggga
    17041 tcgcccagcc cgtgctcggg cacatcgtcg agaaccgctg ggttgtcgcc acgctgctcg
    17101 cggcgctggc cgaggcgccc aacgtgacgg tgcgcggcgg cgagcggctg ctcgcgctgg
    17161 agccggtcgc aggtgtggac gcacggcagc ggctcagcct ggattccggc gcctcggtcg
    17221 aggcggcgct ggtggtcggc gccgatggcg cgcgttccgc ggtgcgcgaa ctgtgcgcga
    17281 tcgaggcgcg ccagcgcgac agcgggcagc aggcgatcgt cgcgaccatc gagaccgcgg
    17341 cagcgcaccg gcgcacggcg tggcagcgct tcctgcagag cggacccctg gcgctgctgc
    17401 cgctcgccac gctcgcgggc gggcacgcct gctcgatcgt gtggagcgcc gacgagccgg
    17461 tcgcgcaagc gctgatgcaa ctcgatgacg ccgcgttcgc cgcgcgtctg acgcgcgcca
    17521 gcgaatcctg cctcggcgag gtgcgcgcgg tgtccaggcg cttcgccttc ccgctgcggc
    17581 ccctgcacgc cgccgcctac gtcgcgcccg gcgtggcgct ggtcggcgat gcggcgcacg
    17641 tgatccaccc gctcgcgggc cagggcatca acctcggcct gtccgatgtg cgcgtgctcg
    17701 ccgcggagat cgtccgtgca cgcgcacgcg ggcgcgccat cggcgatgcc ggggtgctgg
    17761 cgcgctacca gcgccggcgc cgtggcgaga acgcggtgat gctgcgcgcg atggaaggtt
    17821 ttcgcattct ttacggtgac gaccgccccg gcgcgcgcct ggcgcgcaac atcggcatga
    17881 gcgcggtgca cgcgctgggg ccgctgaaac gcgagttcat gcgccgcgcg atgggcatcg
    17941 actgaggacg cgcccgaacg gtagaatcgc cgccgagcgc aactccacct ctttcccgat
    18001 actgggggaa ccgatgagca acatccctgc cgaccgcaag tatgccgtga cccacgaatg
    18061 ggcacaggcc gacgcctccg gcatcgtgac ggtcggcatc agcgatcacg cgcagatcgc
    18121 gctgggcgac gtggtgttcg tggaattgcc cgagctcggc gcgcacatcg acgccggcga
    18181 cgaggcgggc gtggtcgagt cggtgaaggc cgcctccgac atctacgccc cggtatcggg
    18241 cgaggtcgtc gaggtcaacg aggcgctgga gagctcgccc gagctcgcca acagcgaccc
    18301 ttacggcgag ggctggttct tccgcctgcg tgccgacaac accgaggagc tcgacgcgct
    18361 gctcgacgcc gaaggctacg aacacagcct ggacgaggaa tgaggccccc accggcgcgc
    18421 ggagcgcgcg cgtgagcgaa gccggggggt tgccgcgcct gcaattgcgg cgcaacgagg
    18481 accggcgcat ccgccagggg caccactggg tctacagcaa cgagatcgac accaccgcca
    18541 ccccgctgaa gcagttcgcg gcgggcgagc aggtgctgct gtgcaccgcg agcggcaagc
    18601 cgctcggtgt ggcgttcgtg aatccgcatt ccctgatctg cgcgcggctg ctcgagcggc
    18661 acgccggcga ggcgctcggc gcgcagttcc tgcgcgagcg gctgcgcacg gcgctcggcc
    18721 tgcgcgagcg gctgttcagc gagccctgct accgcctcgt ctacggcgac agcgacggcc
    18781 tgtccgggtt gatcgtggat cgctacggcg aggtgctggt ggtgcaggtc gggaccgccg
    18841 gcatggaacg gctggagacc gagctgatcg cggcgctggt cgaactgctc tctcccgcgg
    18901 gcatcctgct gcgcaacgac gcctcggtgc gcgagatgga acagctgccg ctgtacacgc
    18961 gcgtggcttt cggcgaggtg ccggaatacc tcgaactgcg cgagaacggc gccagcttcc
    19021 gggtctcggc cacgggaggg cagaagaccg gctggttcta cgaccaccgc atgaaccgtc
    19081 gccgcgtggc ggagctggcg cgcggacagc gcgtgctcga cgtgttcagc tacgtcggcg
    19141 gctggggcgt gcaggcggcc gtggccggcg cgcgcgccgt gacctgcatc gacagctcgc
    19201 agccggcgct ggagctcgcc gcggccaacg cggcgctgaa cggggtcgcg gagcgcatgg
    19261 attttcgcgc cggcaacgcc ttcgacgagt tgcacgcgct ggcgcaggcc ggcgagcgct
    19321 acgacatcgt ggtgctcgac ccgccggcct tcatcaagcg ccggcgcgac cagaagagcg
    19381 gcgagaacgc ctaccaccgc ctgaaccgcg aggccatgaa cctgctcgcc gacgatgcga
    19441 tcctggtctc ggcctcgtgc tcgatgcatc tcggggaggc cgcgttgatc gacatcgtgc
    19501 gcatggcggc gttccacgcc gggcgccagt tgcagatcct cgaagtcgcg gggcagggcc
    19561 ccgaccatcc gatccatccc gcgatcccgg aaacgcgcta cctgaagtcc gtcatcgccc
    19621 gcgtgcaccg ctgatttgtc cgcgtcggac cgaatgtgga agaataccgc cgtatccggg
    19681 cgtctccggg tgcggcagac ggcggaccat ccgaagtgat cgacgagcag ggataccggc
    19741 ccaacgtggg aatcatcctg gccaacagcc ggggcgatct gctgtgggcg cgccggcgcg
    19801 gccagaacgc ctggcagttc ccgcagggcg gcatccacga gggcgagacg cccgagcagg
    19861 ccatgttccg cgagctcggc gaggaagtgg ggctcgagga gtccgacgtc gagatgctgg
    19921 cctgtaccgc gggctggctc aagtaccgcc tgccgccgca gttcatgcgc aaccgcaaga
    19981 atcccaattt cgtgggccag aagcagaagt ggttcctgct gcgcctggtc tcggaggacc
    20041 agcgcgtgcg cgtggatgcc cacgccacgc ccgaattcga ccagtggcgc tgggtcagct
    20101 actggtatcc gctgggacag atcgtcgcgt tcaagcgcga ggtctaccgt cgtgcactgt
    20161 gggagctggc gccgcgccac cagaggctcg tgcgcaatgg cgcctgagcc cggcgcccct
    20221 gcctcgatgc tgaaatccct gcgccgcatc gtgcaggagg tggtcagcgc gcccgacctg
    20281 cccgagaccc tcgcgatcat cgtgcgccgc gtgcgcgatg tgatgcgcac cgaggtgtgc
    20341 tcggtctaca tccacgaccc ggtgagcgac cgctacgtgt tcatggcgac cgagggcctg
    20401 aacaaggcgc tcgaggggcg cttcagcctg gcgctgggcg agggcctgat cggctacgtg
    20461 gccaagcgcg aggagccact gaacctcgac aacgccgagg cccacccgaa gttcaagtac
    20521 atcgagggct cgggcgagga ggtcttcaag tccttcctcg gtgtgccgat catcaaccac
    20581 cgccaggtgc tgggcgtgct ggtggtgcag cagcgcgaac cgcgccgctt caacgaggac
    20641 gaggaagcct tcctggtcac gatgtcggcg cagctggccg gcgtcatcgc ccacgccggc
    20701 gccgtcggca gttaccgcgg caagccgcgc gacccgtccc ggcgcgccac cgagttcccc
    20761 ggcgtgcccg gctcgccggg catcgcggtc gggcgcgtgg tggtggtgtc gccgccctcg
    20821 gacctcagga gcgtgccgca ccgcccctgc gccgatcccg aggccgaggt gggcctgttc
    20881 caggacgcgg tgcaggcggt gcgcgaagag gtgctggcga tccgcgagga gctgagtggt
    20941 cgcgtgcgcc ccgaggaact gacgctgttc gacgcctacg tgatgctgct ggacgaccac
    21001 gcgctctcgg gcgaggtgat cgagtgcatc cgcggcggcg tctgggcgca gggcgcgctg
    21061 agccaggtgg tgctgcgcca cgtgcgcacc ttcgagatga tggaagacgc ctacatccgc
    21121 gagcgcgcca cggacctgcg cgacctcggg cggcgcatcc tggcgcagct gcaggcgggc
    21181 tcgcgcgggc gcctggagta cccggagcgc acggtgctgg tgggcgagga gattcccgcc
    21241 tcgatactcg ccgaagtgcc cgaggcgcgc ctcgccggca tcgtctcggt gtcgggctcg
    21301 ggcaattcgc acgccgcgat cctcgcgcgc gcgatgggcg tgccgaccgt gatgggcgtg
    21361 gtcgacctgc cgttcgccga gatcgacggc gccgagatcg tggtcgacgg ccacgacggg
    21421 cgcgtgctgg tggatccggc gccggagttg cgccagcagt acctcgaggc catggaagcc
    21481 gagcgcgccg acgcgcgcca gatggagagc tgcatcgatc tgccctgcgt gatgaccgac
    21541 gggcacgcgg tcagactgct ggtcaacacc gggctcaact cggaactgat gcgcacgctc
    21601 gggcgcggcg ccgagggcgt gggtctctac cgcaccgaga tccccttcct gatgcgcgag
    21661 cgcttcccca ccgaggaaga gcagcgcctg atctaccgcg agcagctcga ggccttcgcg
    21721 ccgcgcccgg tcacgatgcg cacgctcgat gtcggcggcg acaagtcgct gccgtatttc
    21781 ccgatcaagg aggagaatcc cttcctcggc tggcgcggca tccgcgtgac gctggaccac
    21841 ccggagatct tcctcgcgca ggtgcgcgcg atgctcaagg cgagcgcggg cctggacaac
    21901 ctgcgcatca tgctgccgat gatcagcaac acgctggagc tcgacgacgc gctcgcgctg
    21961 ctgtcgcgcg cgcgcgccga gctctgcgag gaaggctgga ccatcgcgac gccgcaggtg
    22021 ggcgtgatgg tcgaggtgcc ggcggcggtg taccaggccg gcgatctcgc caggcgcgtg
    22081 gatttcctct cggtcggcag caacgacctc acgcagtacc tgctggccgt ggaccgcaac
    22141 aacgcgcgcg tcgccgcgct ctaccacgcc tatcacccgg ccgtgctgca ggcgctgcag
    22201 gacgtggtgc gcgccgcgca cgacgtgggg cgcccggtca gcatctgcgg cgaactggcc
    22261 ggcgatcccg gcgcggcgct gctgctggtg gcgatgggct acgacatgct ctcgatgagc
    22321 gccaccaacc tcgcgcgcgt gaaggcgacg ctgcgccgct tcgcgcgcgc cgacgccgcg
    22381 cagctgctcg ccgaggttat gcgcatggac acctcgcggc gcattcgcgc ggcggtcgag
    22441 gagtgcctgc ggcgcggcgg cattgcggtg cggccgcggg gttgagccat ccggatggcc
    22501 agctgatgaa cgcccgggca ttcgccagag tcatgacctt gctcgccgcg agggttggca
    22561 aagaacacag cgatccgcta cgcacactga atgtgaacca tgctgggggc tgggtgtcgt
    22621 gggaccaaaa aaagtacggc ttggcccctg cgccttgatg atggttgcgc tgggtggctc
    22681 catgggtgcc gcaggcacta gtttgctgcc tccgttctca catattccgg ttgcgagcga
    22741 gacgttcgaa atcacggtgc cagaaccgtt cgggctgacg caaatccgcc tcgaattcga
    22801 accgggctcg agcaagcgca tttcggtgat cgagataagc tatgccggcg aaacgtaccg
    22861 gatcgatggc ggaatactcg gcgaataccc tgtcgtcgat gaaaccgatt tctactacaa
    22921 cgacagcgac tttcagaacg acgaactgga ttccttctat tttcacgtca accatggtgc
    22981 cgcgatcaag gttgagtgtg gtgtcgaccg gatcgaacac gttcggaaaa tgaagattgt
    23041 gctggtccgc tggcatgccg agcccatcgt tgcggatgac gagtcatatt tcgagacgtg
    23101 cgcgcgctat accgaagcgc agaagcagtt tgaagaaatc aacggaccga tcgagctcga
    23161 ttgagggagc gccccacggg cacaggaggc gcaagtaacc atgtatgaag cgaagcatga
    23221 accgcttctt ccgcgctccg ccttcctgcg gcgcatcgcg ttgcacgtgg gcgccaccgc
    23281 ggccatcctg gcgttctcgc tggccatcgg catgaccggc tacgtggtct gtgagcgcat
    23341 gacgtggatc gacgcgttcc tgaacaccac gatgcttctc ggcgggatgg gtccggtcaa
    23401 tccgccgctg agcgccggcg gcaagctctt cgcgggcctg ttcgcgctct acgcggggct
    23461 ggtcttcgtg gtgagcaccg cgctggtgct gacgccggtg ttccaccggg tgttgcactg
    23521 ctttcatgtc agcgaggagg attgagttca tgcgcgcagc cgtatttcac gaaaccggtc
    23581 ggcccctcag catcgagacc gtccccgacc cgcgtcccgc gcccgaccag gtgatcatcg
    23641 aggtcgcgcg ctcgggcatc tgcggctcgg acctgcacgt gaccgagtac ggcgcggcgc
    23701 cggacggcac aatcctcgga cacgagttcg ccggcaccat cgcggaggtc ggcgccgcgg
    23761 tgcgcggcga ctggaaggtg ggcgagcgcg tcacggcgct gccgatcacg tcctgccagc
    23821 agtgcgaggc ctgcaccggg gggctgccgg gactgtgccc gcagaacatc ttcaccggca
    23881 ccacgctgct ggtgcagggc gcctacgcgc agtacgtcgc cgcgcgcgcc ggcatgctgc
    23941 agcgcctgcc cgccggcgtg cccttcgcgc agggcgcaat ggtcgagccg ctggcggtct
    24001 cgcaccacgc ggtggagatg gcggcgctgc gtccgggggc ctcggtgctg gtgatcggtg
    24061 ccggccccat tggcgccggc gtggtgctgt tcgcacgcct ggccggcgcg cgccacatcg
    24121 tggtgagcga gcgtttctcg gcgcgccgcg agcgggcgct ggcgctgggt gccacggcgg
    24181 tgatcgaccc gtccgcggag gacgtggccg ggcgcttcgc cgcgcacgcg ggctgccgcc
    24241 cgcaggtggt gttcgagtgc gtgggcaatc cgggcctgct gcgtcacgcg gtggaggtgg
    24301 ccggcatccg cgcgcgcatc gtggtggcgg gcgtgtgcct gctcgacgac accttcaacc
    24361 cgctcaccgg gctgctcaag gaagtgacga tccagttcag ccagtgctac accgagcaca
    24421 atttcgcggc cgtgatcgac gcgatggcgc gcggcgaggc gaagccggag ccgatgcaca
    24481 ccggcacggt ggggttcgcg gagctgccgg cggtcttcga gtcgctgcgc gcggcctcgc
    24541 cgcactgcaa ggtgctgatc gatccggacc gctgaggcgc gggctcgata gcgcgcggct
    24601 cagctcgggc gcggcgtcga ttcctgaata tcgaaccggt actccagcgt ctgttgcgcc
    24661 gtcccgctcg catcgaagct gcgctccgtg aattccaccc atccgtcgcg gtgcacgcgc
    24721 acgcaggccg aggtgcgcgt gccgtaatcc ggtgagcaga tgaagcgcgg cccgaggcgg
    24781 cgctcctgct ccagtcccac cccggtgtcg ggcagcgcgt ggtcgggcgg cacgctgcgg
    24841 tcggcgagca gtgcgaggat cgcctcgggt tcggggtgcg tgcagagcag cgccgacagt
    24901 tcctcgcgca ccccgagcag tttgggccac ggcgtgtcga gcatgtcgtt cgacaggccg
    24961 tacacgccgg gctccaggcg ttccatgcgc gggccgtggt tgcccaggta atacaggtcc
    25021 ccgtcgagtt cgccggccag caggttgaag ccggcgtagt cctcgcagct cgcgcgcagc
    25081 cggttcatgt actcgcgcgg cggctcgctc cgcaccagga agtcgagcgt cagcagcccg
    25141 cgcgaaggcg cggccggtgg cgccgggtgg ccgcggcgat tggtgatggc ggcgaagcgc
    25201 ccgccgcgcg tgatgccgag ccaggtgccg ccggccgcca gatcgcgccc ggcgcagagc
    25261 ccggggtgat cgggccagaa atccgccgcc cgtgtcgggc ggacatggaa ctcgtcgcga
    25321 ttggccgcga tcagcagcgg gaattcgggg tggcgctgcc aggccagggc gatcaggcac
    25381 atagatgcgc accaaggggg ggcagggcat aatagcccgc cgccacgccg gccgcctacg
    25441 ccgcggtgcc ctgctgcacc ggcgccgctc gccgcaccgc ccacggagcc tgtagatgct
    25501 gcaccacccg aatttcgatc ccgtcgccgt ccagctgggc ccgctgaagg tccactggta
    25561 cgggctgacc tacctggcgg cgttcgtcgc ggcctggtgg ctggcgcggc tgcgcgcgcg
    25621 ccgcccgggc gcgccgctca cccccgagca ggtcgaggac ctgatcttct ggggcgcgat
    25681 cggcgtggtg gtgggcgggc gcctcggcta cgtgctgttc tacggtttcg atcacctgct
    25741 cgccgacccg acgtggctgt tgcgggtctg ggaaggcggc atggccttcc acggcggcct
    25801 gctcggcgtg atcgcggtga tgtggtggtt cgcgcgcaag cacgggcagc acatcggcga
    25861 catcttcgac ttcgtcgcgc cgatcgtgcc gctggggctg ggcttcgggc gcatcggcaa
    25921 tttcatcggc caggagctgt ggggccgccc caccgacctg ccctggggca tggtgttccc
    25981 gcgcgatccg ctggcgctgg cgcgccatcc ctcgcagctc taccaggccg cgctggaagg
    26041 gctcgtgctg ttcctgatcc tgtggtggta ctcctcccgg ccgcgtcccc gtttcgcggt
    26101 gtccgggctg ttcctgctct gctacgggct gttccgcttc gcggtcgagt tcgcgcgcga
    26161 gcccgatgcc ggcatcggct tcgtcgcctt cggctggatg acgcgcgggc agctgctgtg
    26221 cctgccgatg atcgtcgccg gcctggtgat gttcgtgtgg gcgcagcgcc gcgcgggcgg
    26281 cgccacgccg gccgcgacgg cctgagcgga gcaaggcgat gcaccagtac cagcagctgc
    26341 tgcggcacat actggagagc ggccgcgaca agggcgaccg caccggcacc ggcacgcgca
    26401 gcgtgttcgg ctaccagatg cgcttcgatc tcggcgcggg ttttccgttg ctcaccacca
    26461 agaagctgca cctgcgctcg atcatccacg agctgctgtg gttcctgcgc ggggacacca
    26521 acatccgcta cctgaaggag cacggcgtct cgatctggga cgagtgggcg gacgccgagg
    26581 gcgagctcgg cccggtctac ggctaccagt ggcgcagctg gccggcggcc gacgggcgtc
    26641 acatcgacca gatcgcgcag ctgctggagc agatccgcac caaccccgat tcgcggcgcc
    26701 tgatcgtgag cgcctggaac gtggccgacg tggatcgcat ggcgctggcg ccctgccacg
    26761 cgctgttcca gttctacgtg caggacgggc ggctctcctg ccagctctac cagcgcagcg
    26821 ccgacgtgtt catcggggtg ccgttcaaca tcgcctccta cgcgctgctg acgctgatgg
    26881 tggcgcaggt cacggggctc gcgcccggtg agttcgtgca caccttcggc gacgcgcacc
    26941 tctacagcaa ccacctcggg caggcgcggc tgcaactgga gcgcgagccc ctggcgctgc
    27001 cggtgatggc gctgaacccc gcggtgaagg acctgttcgc gttccgcttc gaggacttcg
    27061 agctgcgcgg ctaccagtcc cacccccaca tcccggcgcc gatcgcggta tgaagatcac
    27121 gctggtggtg gcgatggcgc agaaccgcgt catcggccga gacaacgccc tgccctggca
    27181 cctgcccgag gacctgaagc actttcgcgc gctcacgatg ggcaagccca tcgtgatggg
    27241 ccggcgcacc ttcgcctcga tcggcaaggc gctgcccggg cgcaccagca tcgtggtcag
    27301 ccgccagccg gacctggacc tgcccgaggg cgtgctgctc gccgcgagcg tgccggatgc
    27361 cctggtgctc gcgcacgaaa tcgccgcgcg cgacggggtc gaggagtgca tggtgatcgg
    27421 cggcgccgac atctaccgcc agtgcctgcc cctggcggag cgcatccagc tcacgctgat
    27481 cgagcaggaa gtcgagggcg acaccgtgtt cccggagttc gcgcacaccg actggcgcga
    27541 gaccgcgcgc gagcggcacg ccagcgccgc cgatcccgcg ctgcgcttca gtttcctcac
    27601 gctcgatcga gtgcatccat gaagagatat acggcgaaat ggggcgcgct gggcgcagcg
    27661 gtgtcgctgg ccgcgtggct cggcgcgacg gccgcgcgcg ccgagcagcc gctggaggac
    27721 atctaccgcc tgagccaggc ctccacgcag gccgcgcagg cctcgcaggc gacggtcgag
    27781 cgcatcgccg acgagacccg cgagctgctg caggaataca agaccgtgat gaagcaggtc
    27841 gacggcctgg aggtctacaa cgcgcggctc gaaaagcaga ttgccgggca ggaggagcgc
    27901 atagccgcga ccggggcgtc gatcgacgag gtcacggtgc tccagcgcca gatgatgccg
    27961 ctgctggtgc gcatgatcga tgggctggaa tccttcgtgg cgctcgacaa gcccttcctc
    28021 ctggaggagc ggcgcgagcg catcgccttc ctgcgccaca acctcgaccg ccccgacatc
    28081 gcgctggccg agaaataccg ccaggtgatg gaggcctacc gcatcgagat cgagtacgga
    28141 cgcaagatcg acacctacag ggacaccatc gcgatcgacg gcgagcagcg cgaggtcgac
    28201 atcctgcgcg tcgggcgcat cgcgctgctg taccagacgc ccgaccggca ggccgccgga
    28261 ttctggaatc ccgcgcaggg tggctgggag ccgctggagg gcagcgaata ccgcggcgcg
    28321 atccaggaag gcatgcgcat ggcgaacaag caggcctcca tcgagctgct gaagctgccg
    28381 atccccgcgc cggaggcggc gcgatgaagg cgctcgggag gatcatcacc gcatggctgg
    28441 gagccggcgc catcgcgctc ggtgccgccg cagaggagcg gcagccgcag tcgctgcagc
    28501 agctgctcga gttcgtgcag cagggccggg ccgtggatgc gcgggaggcg cgcgagcgcg
    28561 aggcgcgctt cgtggccgac aaggccgggc ggcaggccga gctcgagcgc gccagggcgc
    28621 tgcgcgcgga gcagcagaag cgctcggccg cgctggaaaa gcgcttcgag gccaacgagc
    28681 gcgagctgga gaaggcgcag gcacggctgc gcgagcggct cggctcgctc ggcgacctgc
    28741 tgggcaacgt caccgcggcc gccggcgatg cccgcgccac cttccaggcc tcgctcaccg
    28801 gcgccgagct cggcggcgcg cgcgtcgcga aggccgacga gctggtgagg ctggcgggca
    28861 gcggcgtcga cctgccgacc atcgagcacc tcgaggcatt gtggtacgag ctgcagcagg
    28921 agatggtcgc ctcgggcacc gtcacggcct tcgcggcgcc ggtgatccgc cccgacggca
    28981 accgggccga gacgcccgtg gtgcgcgtgg gcgtgttcaa catcgtctcc gccgagggca
    29041 agtacctcga ctaccagggt gacaaggggc tgttcaccga gctgccgcgc cagcccggcg
    29101 gcagtttcgt cgacgcggcg gccgggctcg cggcggccac cgggggcgtg caccccttcg
    29161 gtatcgaccc caccggcccc tcgggtggct cgtacctggc ggcgctgatc gagacgccga
    29221 ccttcggcga gcgcatccgc cagggcggcc tggtcggcta catcacgatg gcgctcggcc
    29281 tgttcggcct catcctcgcc gcttaccggc tggtggtgct ggcgcgcatg gacgcgcgcg
    29341 tcgccgcgca gctcgccagc ccgcgcgcca accccgacaa cccgctgggt cgcgtgctcg
    29401 cggtgcacga gagcaatccg cacatggaca tcgagacgct cgagctcaag ctgagcgaag
    29461 cggtgatccg cgaactgccg cgcatcgaga gcggcctcac gctgctgaag atcatcgcca
    29521 cggtggcgcc gctgctcggg ctgctcggca cggtgaccgg catgatcgtc accttccagg
    29581 cgatcacgat cttcggcgcc ggcgatccca aggccatggc caacggcatc tcccaggcgc
    29641 tggtgaccac ggtgcagggg ctgtgcgtgg cgatcccgat cgtgctggcg cacacgctgg
    29701 tcggcaaccg cgcgcgcaag atcacgcaga tcctcgagga ggagaccacc ggcgtcatcg
    29761 ccgagcacac cgggtacgcg caggggaaag actgatgtcc gggctccagg aggcgatgcg
    29821 cggcatcctc gacttcgtcg atgccggtgg cccggtgctc tacgcggttg ccgggctgac
    29881 cttcgtgatg tggacgctgg tgttcgagcg cgcatggtac ctgcgcgtcg gcctgcccgc
    29941 cgacgtgagg gcgatgctcg cggcctggga gtcgcgcccc gagcgccgct cgtggcacgc
    30001 ccgccaggtg cgccgcgcgc tgatctcgcg cctggggcgg agcatcgggc gcaacctcgc
    30061 gctgatccgc gcctgtgtct ctctgtgccc gctgctcggg ctgctgggca ccgtgacggg
    30121 catggtggcg gtgttccacg tgctggcggt cagcggcggc gatacccgat cgatggccga
    30181 gggcgtctcg cgggcgacca tcccgaccat ggcgggcatg gtggccgcga tttccggcgt
    30241 cttcgccaac ctctacgtca cgcgcgtcgc ggcgcgcgag ctgctgctcg ccgaagaccg
    30301 cctgaccacg gaccactgag ccgccgccat gcgccgcaac cgggtgttca gccgcgagga
    30361 gcgttccgcg ccggggcaga tcgatctcac gccgatgctc gacgtggtgt tcatcatgct
    30421 gatcttcttc atcgtgaccg cctcgttcat cgaggaggcg ggtatcgagg tcgagcggcc
    30481 cgaggcggtg accgcgcagc agaagaagag cacgcggatc ctggtggcgg tgtcggccga
    30541 cgaccaggtc tggatcgcgg gccaccgcgc cgacccgcgc gcggtgcgcc gcgagatcga
    30601 gcgccagctg gccgcgagtc cgcacggcgg gctggtggtg caggcggatg cgcgctccag
    30661 cgcgaaagcg gtggcggcgg tgctggacgc ggcgcgcgcg gccggcatcg cggacgtgtc
    30721 gctgtcgacg cgccggcact agcgtgatgt accggtggcg ctacctgttc gcggcggcgc
    30781 tcggcggcgc ggtcacgctc gcgctgctcg cgctgatgca cgcgctgatc gccaccggcg
    30841 aacgcgccct cgaggagagc acgccgatcc ggatcgcgga tgtcacgatg cccgacgcgc
    30901 gcatcgagtc ggggttcgtg gcgcgcctgc ccgagcggat cgaggagccc gagcgcccgc
    30961 cgcccgccgc agcgctgccc gaggatttcg ccgctgctgg cgctctcgtg cccgacatga
    31021 aggcagcgct cgcaccgcag ctgcagatcg gcaccgggcc cggcgccgtg gcgacggacg
    31081 gcgagtacct gccgatcgtg aagatcgccg cggtctaccc gcgccgggcc caggagcgcg
    31141 gcatcaccgg tcattgcatc gtcgaataca cggtcaccac cagcggcgcc acccgtgatc
    31201 cggtgcccgt ggactgctcg ccgccgggcg tgttcgagga gagctcggtg aaggcggcgc
    31261 tgaggttcaa gtaccggccg cgcgtcgtcg acggccagcc ggtggaggtc tccggcgtgc
    31321 gcaacgagtt cctctacgag ctcgacgaat gaacggggcg ctgcagggcc ggcaacgcgg
    31381 ggcgatacgc cgcggcctga gccggctggg cgttgccgcc gcgctcctcg cattgccggc
    31441 cctgctcgcg ccggcgggat ggccgggcgc gtccagcgcc cgcgccgagg atgcgcgggc
    31501 ggggcaagcg caggggacgc ggcgcacgcc gacgctgcgc aacgaggtcc acgcgaagct
    31561 cgcggacgtg cagaaggccg ccgaggccgg ggatttcgcc accgccgagc gcctgctcgc
    31621 ggcgctgcag gccgggtttt ccggcaagcg cgcgctgaac agctacgaac tcgccaacgt
    31681 ccacaacttc tacgcctaca tctactactc gcagcagaaa caccccgagg cgatccgcgc
    31741 ctacgagaaa gtgctggcgc agcccggcct gcccggggcg atggagaccg gcacgcgcta
    31801 cagcctggcg cagctctacg tggccaccgc ggactggaag cgtgccgccg cgatgctgga
    31861 gtcgtggttc aggatcgccg aggagccggc gcccgaggcc tggatgctgc tggcgcagac
    31921 caactaccag atcggggagt acgaccgcgc gctcgggaac atcgacaagg gcatggcgga
    31981 ggcgcgccgg cgcgggatgg cgccgaagga gaactggtat ctgctcgcgc gcatggtcag
    32041 ctacgacaaa ggtgacctgc ggcgcacggc ctcggtgctg gaggcgctgg cgcgacagtg
    32101 gccgaggaag gactacttcg tgcagctctc ggcaatccat ggcgagttga aggacgagcc
    32161 gcgccaactc gccgcgatgg aggtggccta cctcgccggc tggctgggcg aggaacgcga
    32221 gctggtgaac atggcctatc tgtacctcgg cgccgatatg ccgtggaagg ccgcgcgcgt
    32281 tctggaacag ggcatggagg ccgggaaggt cgaggccggc gcgaagaacc tggagttgat
    32341 cggcatcgcg ctgcgccagg cgcgcgagaa ccgccgggcc atcgtgtacc tcgagcgcgc
    32401 ggcggcgctg gcgggcgatg cggaaatgtg ggcgcggctg gcgaacctgc agctcgacga
    32461 ggacgccagc gaacaggccg tcgaggccgc gcgcaaggcc ctggcgatgg gcggcgggcg
    32521 ccgccccgat cacacgcagg tggtgctcgg catggcgctg tacaacctcg ggcgctacga
    32581 cgaagcgcgc gcggccttca ccgaggccgg gcacgacccg cgctcggagc aagtggccac
    32641 gcagtggctg cacttcctcc agaccgagat tgcccgcgcc gcgcagctgg cgcaggaact
    32701 gtaggtcggg cccgacctac aggatcttgt cgagatcgcg caccgcgccc ttgtccgcgc
    32761 tggtgcagag catcgcgtag gcgcgcagcg ccgccgagac cttgcgcggc cgtgccctgg
    32821 cgggcttcca cgcggcagcg cccttggcct ccatcgccgc gcgccggcgc gcgagttcct
    32881 cgtcgctcac cagcgcctcg atggtgcgcc ccgggatgtc gatgcggatg cggtcgccgt
    32941 tctcgatcag cgcgatcgcg ccgcccgcgg ccgcctccgg cgagacgtgg ccgatcgaca
    33001 gccccgaggt gccgcccgag aagcgcccgt cggtcagcag cgcgcaggcc ttgccgagac
    33061 ctttggactt caggtagctg gtggggtaca gcatttcctg catgcccggg ccgccgcgcg
    33121 ggccctcgta gcgcacgatc accacgtcgc cttccttcac caggccgtgc aggatgtgct
    33181 cgaccgcctc gtcctggctc tcgcagatgt gcgccggccc ctcgaagacc cagatcgatt
    33241 catcgacgcc ggcggttttc accacgcagc cgtcgcgcgc gatgttgccg tggagcaccg
    33301 cgagcccgcc ttcctgcgcg taggcgtgct ccgcgttgcg gatgcagcct tcggcgcggt
    33361 cgaggtccag cgtgtcccag cgcgtggact ggctgaaggc ggtctgcgtc gggatcccgg
    33421 ccgggccggc gcggaagaat tcgtgcacgg tcgcatcctg cgtgcgcacc acgtcccagc
    33481 gctccagcgc ctcgtgcagc gtcttgctgt gcacggtggg cacatcggcg tgcagcagcc
    33541 cggcgcgctc cagttcgccc aggatcgaca tgatgccgcc ggcgcggtgc acatcttcca
    33601 tgtggtactt cggcgtgctc ggcgccacct tgcacagctg cggcacgcgg cgcgagaggc
    33661 ggtcgatgtc ggccatcgtg aagtccacct cggcctcgcg cgcggcggcc agcaggtgca
    33721 ggatggtgtt ggtcgagccg cccatcgcga tgtccagcgt catcgcgttc tcgaaggccc
    33781 ggaagctcgc gatcgagcgc ggcagcgccg aggcgtcgtc ctgcccgtac cagcgcttcg
    33841 ccagcgccac gaccagtcgg ccggcttcca ggaacagccg ctcgcgatcg gcgtgcgtgg
    33901 cgagcgtgct gccgttgccg ggcagcgaga gcccgagcgc ctcggtcaga cagttcatcg
    33961 agttcgcggt gaacatgccc gagcaggagc cgcaggtcgg gcaggccgag cgctcgtact
    34021 cggcgacggt ctcgtcggcg gccgcgtcgt cgaccgcgat caccatcgcg tctaccaggt
    34081 cgagcttgtg ctcggagagc ttggtcttgc ccgcttccat cgggccgccc gagacgaaca
    34141 ccaccgggat gttgagccgc agcgccgcca tcagcatgcc gggcgtgatc ttgtcgcagt
    34201 tggagatgca caccagcgcg tcggcgcagt gggcgttcac catgtactcg accgagtcgg
    34261 cgatgatgtc gcgcgagggc aggctgtaga gcatgccgtc gtggcccatc gcgatgccgt
    34321 catcgaccgc gatggtgttg aattccttgg ccacgccgcc ggccttctcg atctcgcgcg
    34381 ccaccagctg gcccatgtcc ttcaggtgca cgtggcccgg cacgaactgc gtgaacgagt
    34441 tcgccaccgc gatgatcggc ttggagaagt cctcgtcttt catgcccgtg gcgcgccaca
    34501 gcgagcgcgc gccagccatg ttgcggcctt cggtggtggt cttcgagcgg tacttcggca
    34561 tggtggtgtc ctgcacgcgg gtgattcggc ggccgcaggc tgcggcgcgg cgcatcattt
    34621 tatgcccttg gcacgctttc tcccacccgg atgaataccg cggagcgtag gtccggtttc
    34681 agccggacaa tgtccgcgtg tatgcggacc tacgccaggt cct
//
LOCUS       DAORUQ010000011        31731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_963_length_31731_cov_6.532169, whole genome shotgun sequence.
ACCESSION   DAORUQ010000011 DAORUQ010000000
VERSION     DAORUQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 31731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31731
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_31731_cov_6.532169"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..973
                     /locus_tag="PLW13_02100"
     CDS             428..973
                     /locus_tag="PLW13_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQY69187.1"
                     /translation="MAPVAPQRFAQIALHWLVVVMIVAIYVSMECRGWFPKDVRPTMV
                     RVMHYSFGITVLLLVPWRLALRVGRPMPPITPAPPRWQEQGARIVHLSLYLFMIAMPL
                     LAWLSYSLRGKPVWFYGFDLPGLLPPADAVLRKQLRLMTWRKRIAEAGYWLLGFHAAA
                     ALLHHFVTGDDTLRRMWFRRT"
     gene            1068..2864
                     /locus_tag="PLW13_02105"
     CDS             1068..2864
                     /locus_tag="PLW13_02105"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQY69188.1"
                     /translation="MKSADDLRIFARTIRWLPAMLRARPGKRYTSADLVESSARRFGD
                     RVFVEFEGRTTSYAEFNRYANRVAHWAIDRGLRRGDVVALIMENRPQYLGVWAGLAKV
                     GVTTALINTHLGGHALEHVIESAACRVVVVGDECLPAYRDIASAHVRALPVYLSRRDG
                     AAVDPAEAGFASLDRELGACPTDNPPASVRAGLQSGDPLFYIYTSGTTGLPKAARFSH
                     ARFVAGGSYHLWSGFGARDALYCSLPLYHTVGGVICVNAVLLTGARLVLRRGFSASRF
                     WQDLAESGATTFQYIGEMCRYLLAQPPGEYDRRHRIRYAVGNGLRPDIWAEFTRRFNV
                     PRMVEFYGATESNVTLVNLQGRPGSVGRLMPGITAALVRFDVEKEAHVRDADGLCVAC
                     AVGEPGELLGRIASGNKPELVRFEGYTSAEATRAKIVHDVLQHGDAWFRSGDLLKRDA
                     DGFYYFVDRIGDSFRWKGENVSTQEVEESLAAFPGVDVAVVYGVEVPGAEGRAGMAAL
                     ALSRAIDLSGPEFYHHVERLPAYARPVFLRVIGEIEMTGTFKVRKVELQRQGFAPGDD
                     ASPVYFRDDAAGCYRVLDDATYQRLRAGELRL"
     gene            complement(2854..4383)
                     /locus_tag="PLW13_02110"
     CDS             complement(2854..4383)
                     /locus_tag="PLW13_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69189.1"
                     /translation="MLQLSESANLFVSTETDTGYQHTAGLVILDAGASRDFCFEKLKA
                     FVVERIDPIPQFRWKLREVPFGLDLPYWVEDDQYSIDRHIHRIAVPAPGDMRALTELA
                     GYLYSRRLDRSKPLWELWFIEGLAGRRYALLQKLHHCMMDGQGAQKIGEALCDFEADP
                     PPRPIPPEFLGAATGGAPSELQLYARTVGNLLQAPARAGRHALSMLRPAIAALGQPEK
                     PRSGARPAAAPALPCNGVIGKERGFACLSLPLADIKRVKNRFEVSINDVVLALTASSI
                     RNYLLHLGVLPRESARANIAVSLRKEGDDAASNAITSVNLALHTELADPVERLMAIHS
                     ETGAAKQAARSGRSSIYELINSLPPFALAVLNKALTPAMMLDTMRCNFIVSNVKGSAR
                     PMYVAGARLEAMYPMSLLANGMGLNFTCVSYVDNIDFGIIYDVDLVPDAWRIAEGLRE
                     ALAEYLRFDTRAKPRRAEDVGRVKRGKGVAKRAAGPARKVTPPRAAKRRQTVTPRRPR
                     A"
     gene            4583..5482
                     /locus_tag="PLW13_02115"
     CDS             4583..5482
                     /locus_tag="PLW13_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69190.1"
                     /translation="MNREAVSSDIPAASILDLPPLATVRVLDAEICYREASGPANAPV
                     VLLVHGILATSGLNWMHAFAPLSRHFRVIAPDLCGHGGDRFHRRRFTLEDCADDIAAL
                     IAVLGLKRVIVVGYSMGGMVAQYVARRHGGLISGLVLSGVDWGSPQYGRATQLVGPAV
                     MEAAVRLVHLQVCLLRAPVALLQRLGGAGSGRREPGPLRVSMGEFTGHNPRAIGGAVR
                     STMRFRSLEWLHEIRVPTTVLVTLRDRLFPQAEQRRMAEAIDGARVSEFDGGHVAVRE
                     PAYATALLEACRELSARMARSAR"
     gene            5514..6920
                     /locus_tag="PLW13_02120"
     CDS             5514..6920
                     /locus_tag="PLW13_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69191.1"
                     /translation="MFRLSGHDAYFLYQETQTTPMHTLKILIGRHTGGTAPGLREIKQ
                     SIGASLDQIPGFRRRIVPVPLGLHHPVVIEDPDFDIDLHVHRLAAPEPGGAAELDEVI
                     AQVSSHPLDHSRPLWELWMVEGLEGGRVAFVVKLHHAIADGQASVNQFERMLGAGGQE
                     PAPAMPVPWQPEAVPSPGRLLRDALRDQLMDIWRLPALIGQTVRTLHRIYLRHRARAL
                     DIPLPSKVPPTPFNRSLCGQRDFATVRFALEDIKAIKSGLGGTVNDVVMAMVAGAVRE
                     YLLERKRLPAQPLVASVPAAGVEQAGAKRVFGNTASGVTVYLRTDIGEARARYAATRE
                     AMAASKELLELLGRNTFHSWADYIPPLLYTALQRLKVHFRLAERRAGAMNLIVSNVQG
                     PRQGAPMPGFDVEELISGGPLLEGVALNITAWSFNGRLNFGLVACRRAVPDLPALARL
                     LQRQLAALQELAGAGSAP"
     gene            6917..7756
                     /locus_tag="PLW13_02125"
     CDS             6917..7756
                     /locus_tag="PLW13_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY69192.1"
                     /translation="MSATRRWTTIEPASGRKLAVSAGSGRGVPFVWGHALQCSMRADA
                     ASGVFDWSGLAPAVRVIRYDARAHGESCSEADPRGCAWESLARDMWRVAEDCGADFPV
                     LGGASMGCATAVWAAMQHPRRVAGLVLVIPPTAWEWRPRQVRSYLMMARLVRFAGRLP
                     FRAASALLGDRPPDGPLKAIAREVVRDLATRDPRDAEVAFKGAALSDLPSPERCARLR
                     MRTLILAWRNDPAHPVEVAQRLAEVMPNARLEIAESERDVRRWPDAVGNFLGKAGERR
                     RKA"
     gene            7847..8785
                     /locus_tag="PLW13_02130"
     CDS             7847..8785
                     /locus_tag="PLW13_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69193.1"
                     /translation="MLQQEGGNEMNRIIPPRFEGTFRLPDGRKLGYAEYGPVDGKPIL
                     WFHGTPGARRQIAPEARRLASRRKVRLIAVERPGIGDSTSHSYGSLADWAEDIRVFCD
                     AKGIKEFAVCGLSGGGPYALACAFYLQGRVVAATILGGVAPARGDDAVEGGVSTLARR
                     LSPVVRHLRVPVNRLMLRLIRRLEPHADKAMSLFARFMPPGDKVMFEDPELQAVLTDD
                     LLLGSRTQMQAMIHDVVLFARDWGFPLGKIETPVYLLYGNADNIVPVEHGRHMAVRIP
                     NAVFREREGEGHMGGLGASEEIFNDLLSHWPRRRRE"
     gene            complement(8846..9970)
                     /locus_tag="PLW13_02135"
     CDS             complement(8846..9970)
                     /locus_tag="PLW13_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY69194.1"
                     /translation="MDATNMEQYTDRLMAGQLELMRRYPSFRMDIYPTRRGFWLPERI
                     QQKSLANARNPECRTSANGVGVYGCWGGTPFPVPQNGYQAMWNHLLFNIPTGKLTADS
                     YLVNANGAISLLVINSAIGDNPYFNPEQKPYEGAGRYYLRGFATNIEPVREAGSQTLV
                     WYPLQFDVDDQRAWTYQAGQRRVRMAPEFSYDTPIAHIGGALFYDEVQMFAGRMDRFD
                     FRLTGRKLMYLPCNNYRMHLLRGDPAQLLGPHHVKPEQWRFELRRVWVVEATPLPGAR
                     HMAAKKRFYLDEDSWVALAYEAWDHSGKLYRVVFGNGLADYAHGGYFMAESVQAYDLV
                     RGQYAVMQAHLCSHCYDRRGVSFLPDAEMTPARMGGRGIR"
     gene            complement(10456..11943)
                     /locus_tag="PLW13_02140"
     CDS             complement(10456..11943)
                     /locus_tag="PLW13_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69195.1"
                     /translation="MRKPRQLKPQDVMFVGGENAHVYQHTMGLILLDSSGRPGFGFGF
                     DALRRHIETRLSSVPQFRWKLHEVPLGLDLPYWVADENFSFDHHIRRIAVPSPGDQRA
                     LGETVAYLYSRHIDRSRPLWEIWFIEGLADGRFAVAQKMHHCMMDGTGAMKQMDHMWD
                     LEPDAAPRPVDRATAEALPGDVPEQWQLSLNAARHLYGMPMQIGRGLYDVAWRSLAKR
                     FSATDDASKKRPTAPTAIFNADITGDRGFVFGSVPLRDIKTVKRHFDVTVNDVVLAVV
                     GGSLRDYLLARAALPDEALRSSIAVSLRTDADDEFSNRVTTASVTLATDLADPVQRLR
                     AIAAEAVAAKEEAHHGGKGVLEFVQALPPVLVGTIMRMAPADQVSRLMGVNVIVSNMR
                     GSAAPMYVGGARVSAIYPMSILAPGGGLNITCLSYADEIHFGVAIEPNLVPDPWRIID
                     GLHTALAEYVALTGTGARRGKVGTGKPGMRKRKRATVRKTAAATP"
     gene            12320..12712
                     /locus_tag="PLW13_02145"
     CDS             12320..12712
                     /locus_tag="PLW13_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69196.1"
                     /translation="MSVTNHQSGTNVHEVADGIYSINTPVQLEGGVGFSFNQHLVLDD
                     EPLLFHTGPRRMFALVREAVAHVMPVERLRYLAFSHVEADECGALNERHAAVRRPVHA
                     GRHRPAAAHRVGHVDFREDRERSSDLGR"
     gene            complement(13045..13500)
                     /locus_tag="PLW13_02150"
     CDS             complement(13045..13500)
                     /locus_tag="PLW13_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69197.1"
                     /translation="MKHILRIALLTAVAAQPALAATEKVNVCHKGTETLSISQSAVRA
                     HTNHGDALGACPVIPPTLASVAVLRCDSVAGSVVVVAASASEQDAGLVQLPVTLGDNC
                     ATGVADLLNSGWELKFVSAGSAVAPGEGASGFVTEYTFVSTQPQTVVAP"
     gene            13590..15479
                     /locus_tag="PLW13_02155"
     CDS             13590..15479
                     /locus_tag="PLW13_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69198.1"
                     /translation="MNAGCIWTSRDSVVAEGLTAQMISSAMPTRLSRHFAVAVLLAAA
                     CLVAGCAATRQPVDTDATHRIDPGARWQINADRWNATFEMSPHAAYGWSGGDAAISLA
                     LPYCPECPAGSHHRRLWLFGDSAFSTVDTEGYRVKADGERGADYTFGNVAAITAHAAP
                     SAPGADTVIFDYGARGFRSNSWMPLLLDIRQLPDMDDLRIAAAGYADLGFARAPQPVA
                     GVYSLVELAPEDLRSDLVAVNEYYDPASRASDYITPDVAVHGLPGTYEYRRVSFYIHR
                     SPQQGTVPLYRYRFAAHGDVVLSTSARAPVGAGSGAGQLLGHVYAAGSAPRNVHALVE
                     YSRVATAHHAAQHAYTTRPNDAGKDILMWPNRGLVIGNDLVQVFSPVTPLDLAGMVPW
                     DDIDRNVVSIVRGVDRPYAQWGKTPQGDWLVSPRQVALRHSDARTSWGLFLLKDEHFE
                     RNRHVYVYGRREGKLVLARVACRDANDFVRFGNWRFYSDGRWVESQEDATPIADYVAT
                     DFSIHESPAGHFVLIQSPPGLVPAIAVAVASSPAGPFRAGPDVDLSGYRNEADRHRRY
                     SYYAVSAHGGLSVGGDRGGLLISYVRSCAFSADAACTGKDADENRADVYVPRFIDLPW
                     RVIDP"
     gene            complement(15445..17844)
                     /locus_tag="PLW13_02160"
     CDS             complement(15445..17844)
                     /locus_tag="PLW13_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQY69199.1"
                     /translation="MYDDTLTTRAAAIVAGLTAAQCIALVGGRDMYSTRELPGLVASA
                     TMTDGPHGLRKQVGDSDHLGLANSVPATCFPVATVLASSWDVELLHEIGRALGRECRA
                     EDVAMLLGPGLNMKRHPRGGRNFEYFSEDPVLAGYLAAAIVNGIQSEGVGACAKHFAA
                     NNQESSRMIVDALIDERTLREYYLRGFEIVVERSRPAAIMTAYNRINGTWCSDNAWLL
                     RDVLRGEWGFGGLVVSDWGGTNDRVAALRAGMDLEMPGNGGAFTPEIVAALASGALAE
                     ADLRASATRVAALALRADALRERAVEPVDFEAHHDLARRAAAAGTVLLSNDGFLPLGD
                     TAGVALIGAFAVEPRYQGAGSSRVNPARLDTLRDALGIELPYAPAYDPATGETSAQLL
                     AQAMAVATAARRVVLVVGTPPRLETETRDRPTTRLPRAMDELVASVCRANPRTVVVLM
                     NGGSLELDWAGLPAALVESYLGGQAAGSALAEVLLGKAEPGGRLAESLPVATSGLPAD
                     ANFPGLPRQVQYRECQWIGYRFHHTFGVPARFPFGHGLGYTTWSLGALRVRGEGTART
                     ATLTITNTGARAGTTVVQLYLRAVAPVLPRPDRELAGFARLRLEAGATAQLDVVLEPR
                     VFALWDSAAGRWVVAGGQYEVLAGTSSVEIHDRVMIDVESTDTITPGAPLAAPIASDA
                     GFAALLGRPIPAAEGPRPFSRTSTVTELSASRLGRVLAALMRRQMDRMIPGDDDGSLR
                     ELMESVVASMPLRGLAGMGPGLTPAMLDRIIAALNGQWIRAIRGKPTVVRDRSRAMAD
                     R"
     gene            17938..18669
                     /locus_tag="PLW13_02165"
     CDS             17938..18669
                     /locus_tag="PLW13_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69200.1"
                     /translation="MREAVCLRVCHAIAVCACYRWRAASKQAQTAILHENFRMSLLNP
                     VPPLAALFLTLVLAAQAPCVHATTIVQRDIREVAEGATLVVEGTVTAVESAPVMQGRA
                     IASTVTIAIEEVLKGSWSAESIDLQYLGGSHDGRSMSVGEMEIPAVGEHGIYFIRDPR
                     RQFVHPLKGWEQGHFIVVTDGPQGEETVRAGNGKAVVGLPADAAPPAQGQVIGKRVAA
                     GLRTADGPDEHALSAAAFRDWIRSR"
     gene            18689..19813
                     /locus_tag="PLW13_02170"
     CDS             18689..19813
                     /locus_tag="PLW13_02170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HQY69201.1"
                     /translation="MRQHALVIALGAALCAPPGADAYEIGFPYHNPVIDMQVDIAGSS
                     PSGTRWNAAFAEAARRWNSDAGVRIDVVNRYSDPCAGVVPLTVNDPLNGVALRDDLCG
                     FAFDEDTLAVTLRMFQLYSEVTMVEADIVFNDSVAWDVYDGPLRSGRPDFRRVATHEL
                     GHVLGLEHEDDSPALMNSAISGIYAPNDDDRNGVSALYGSLAAGDGNIELMIEEPAQG
                     STVAGVGNIRGWALGDSNILSVELYIDGRFIADIPHGGTRNDVRDAFPVRPGSAEAGY
                     SMAFAWSLLTPGVHQLRIRAIDFEGRAAEATGSFTVNAFGTEFIGEPEKVSVNGGVEK
                     LDARTIRLNGVQADGLLYDVKLQWQPASQKFEIVEIEPAD"
     gene            complement(19839..20696)
                     /locus_tag="PLW13_02175"
     CDS             complement(19839..20696)
                     /locus_tag="PLW13_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HQY69202.1"
                     /translation="MKIPRALEPLIEDGLVDDVIRQLKSGKEATVYLVRCGREIRCAK
                     IYKESDKRSFRQAVEYQEGRRVKSSRRARAMEKGSRFGRKEKESAWQSAEVDALYKLA
                     AAGVRVPKPYGFFDGVLLMELVADGEGNVAPRLAEVELAPEQAAAFHHTLIGEVVRML
                     CAGIVHGDLSEFNILVDDDGPVIIDLPQAVDAARNNHAKRMLERDVANLADYLGQFAP
                     QLRGTDYGNEIWAHYEAGTLEPDTPLTGEFAQPTELADVEAVLKEIEDVRAEAMRRQE
                     WLAAEQAEN"
     gene            20938..21942
                     /locus_tag="PLW13_02180"
     CDS             20938..21942
                     /locus_tag="PLW13_02180"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQY69203.1"
                     /translation="MTHKRTIVDLIGNTPLLYLRQVSELTGCDIYGKAEFLNPGGSVK
                     DRTALGIVRDAEARGVLRPGGVIVEGTAGNTGIGLTLVANALGYESVIVMPQSASQQK
                     IDQLEMLGAELILVPACPYSSPGHYIHVAERVAEGRSHQEAHGAVWARQFDNPANARV
                     HFDTTGTEIWAQTEGKVDAFICAVGTGGTLAGVAAALRERNPAVKIGIADPHGSALYS
                     YFTKGELAAHGESIAEGVGVSFVPGNLQGTAVDLAFAVDDDTALPFVYGLLKHEGVSV
                     GPSSGINIAGAVAMARELGPGHTIVTILCDSGSRYLDQLYNAEFLWSRKLPVPDWIVA
                     "
     gene            complement(21950..22897)
                     /locus_tag="PLW13_02185"
     CDS             complement(21950..22897)
                     /locus_tag="PLW13_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69204.1"
                     /translation="MSYRFDPELAPAVPFLPQLDFSDVAVARATITAMSAQMPAPDTS
                     AVHIENRTIEGPDGPVPVRIYRPLAQQGAVAGLLYIHGGGFVVGSLETEHGNAIALAS
                     SLGIVLVSVGYRLAPEHPYPAGLHDCEAAFLWSVANAAELGIDVARLGVFGQSAGGGL
                     AAALCLLRRDRGGPQPCFQFLGIPELDDRLQTPSMRAFTDTPLWNLPAAELSWRYYLG
                     ELAPGSAAVPSHAAPARASDLAGLPPACVTVMEFDPLRDEGLHYAQRLLQAGVSVELH
                     AYPGTFHGSSLITDAAISRRQWADSCAALARGLGLPPAG"
     gene            complement(22927..24417)
                     /locus_tag="PLW13_02190"
     CDS             complement(22927..24417)
                     /locus_tag="PLW13_02190"
                     /inference="COORDINATES: protein motif:HMM:NF005508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY69205.1"
                     /translation="MSEIRASLRGNAPELLRAADVRQWTRSTDVLVIGYGAAGASAAI
                     AARESGAAVLVLERTSGAGGTSGIAGGHLYLGGGTPVQQACGFADSPEEMYKYLEALT
                     PDPDPLKLRLYCEHSVAHFHWLEAHGVPFDRSYYPGKATMQPGRECLIWSGNEKVWPY
                     RDLAQPAPRGHKVAFDGPDGGGGLLMQQLAQAALQAGVATQFDTRVTQLVADDDGRIV
                     GARALCDGSELYIAARGGVVLAAGGYIMNEDMFAHELAWMPRDLIRQGSPGDDGAGIL
                     LGLSAGAAACGLDRAFLTSPFYPPESLIKGILVNAQGERFVAEDSYHGRSAGFIVEQP
                     QGIVYLVLDSATFGYPPYDFFEQRLVDGFETVAEMEARLELPAGSLVRTLADYNAHAA
                     RGEDPAFHKYKDWLQPLDQGPWAAFDLSVGRAKYGGFSLGGLSVSADGEVRDDSGGAI
                     PGLYAAGATAWNIAQDARGYSSGTCLGEATFFGRRAGGACARRINP"
     gene            24777..26000
                     /locus_tag="PLW13_02195"
     CDS             24777..26000
                     /locus_tag="PLW13_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69206.1"
                     /translation="MTDKAAQRLLSGRAWDDFCEQVRLAGHMVDRFGDSADALDRAEW
                     YRFVTRLMRNGFERFMENCEPERPRLRDAPWRQGINFQSPDQDHLLAEFVDGSHDYRI
                     TGNRGTLPYFVMAAWSAGQPADIGARDWAPRGVEGLAEFDPAMLRTTGFLQSDAIHFD
                     AEGNFEVIASREKPADGSDWLPIAPDCVGILVRAVYHRREQTRAPVMRIERMDGALPR
                     PLRPDEVSAGLAKSAQMVLGYAELVRSWWQDNLGARPNRIRFSRAVYLSNGGVPDRHH
                     GFGTWERAGDEALVLSFVPTPCDYWIFQLCNIWQENLDNYEDGDGYVQKYTAAYEPDG
                     SVRIVVAEEDPGTGGNWIRPFGHVHGGMSLRLIKTAGEPPAVTLHRLPLARLREQGLA
                     ALDGLAPIVSGEVDD"
     gene            26084..>26532
                     /locus_tag="PLW13_02200"
     CDS             26084..>26532
                     /locus_tag="PLW13_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69207.1"
                     /translation="MSDTIHNTLRFDYRGARVLVTGGTSGIGAAIAAAYREAGAEVTI
                     TGTRAAAADYDEDLRGYRYLQLDVEDAAQVASVAAAQQQLDILVNNAGLALPSLGLDE
                     YEPDVFARAVNMHLVSAYRMAKGCQPALSASSMPGGASIIGIASMSS"
     assembly_gap    26533..26632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26839..28221)
                     /locus_tag="PLW13_02205"
     CDS             complement(26839..28221)
                     /locus_tag="PLW13_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY69208.1"
                     /translation="MTAEQAAFPSAAVAWIEQQLGGRVAALERMVVRREAWRADIERD
                     DGSVQPVFLRIDRELAAGRPYPRNLARESALIRHLAGHDDIPTQQLHAWSPEHCVAIQ
                     SFEPGRADLNNASRAEQHDVMLHFMEIMAAMHRLDVRTMDLPQFEMPATPEDHSLLEL
                     RAVDDPRFAKLPVDSSAVLGAFGRRWLLNHVPRTVERTVLLQGDTGPGNFMFENHRVT
                     AVVDWEWAHYGDPMEDIGNLWLRDFFTPSCAGDLTPYVRHYAARAGISVDRQKAIYYL
                     AHQVVRSVITIPALVQRPDWKSTVALNLGYQAVCDLVCCEAIGMYHGMPEPDTEILEI
                     PGRNPAGELYEVLAKQLERGIAPHVEDAFAKATAEGAARIARYLERRHLHGAQADALE
                     LAGIDALLGTRHRDLASARLEMKHALERIPPGREPEVLRHLWGVARRNMQLMGPLVER
                     WSECRLARLD"
     gene            28531..29025
                     /locus_tag="PLW13_02210"
     CDS             28531..29025
                     /locus_tag="PLW13_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQY69209.1"
                     /translation="MSASSGSEPSVSRLVTEISRLFSRDFSARVRDYDLTHTQWQALA
                     VLSRQEGINQAELANLLQVQPISLGRLIDRMEAAGWVERRPAPNDRRAIQLYLTSRVE
                     PILDKMTEAASLTREAALADFATDEREQLCRMLQRVKANLAGDDPLATAVAPPQAGRR
                     TSNR"
     gene            29022..30281
                     /locus_tag="PLW13_02215"
     CDS             29022..30281
                     /locus_tag="PLW13_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQY69210.1"
                     /translation="MNAEAARKPESAGQRAGTLQSVGTPVSAAPVADGGAGSAATTAG
                     VLPTDATALEPYSPRPRLRRRLLVAGPLIVALAGLLLWFSTGRYASTEDAYVKADKVT
                     ISAQVAGPIAGVAVRENQRVRDGDELFRIDDRPYRIALAGAEAELAAVATELDGIKAS
                     LRQKNEELRLAHTNRDFAAREFERQSELARKKLNSGAAVDEVRYKLDVALQQISVTEQ
                     ARAQHLSRLNGNPELPLEQYPAYQAARASRDDAALDLERTVVRAPFAGIASKTPQRGQ
                     YVAAGSAVMSVVGADEVWIEANYMETDLTHVRVGQPVSVQIDSYPGRHWRGTVQSISQ
                     ATGAEFSVLPPQNASGNWVKITQRIPLRIAIEPRADDPPLRAGMTATVAIDTGDRHTL
                     KALLGGIGGSVDDHRAAIPDGSTHSAP"
     gene            30278..>31731
                     /locus_tag="PLW13_02220"
     CDS             30278..>31731
                     /locus_tag="PLW13_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HQY69211.1"
                     /translation="MSVAGDAEHVPTKHPWLLLCTMMLTTVMVALDSTIANVVLPHMQ
                     GSLSAAQDQIAWVLTSYIVATAIFIPPTGYLAACLGRQRLLGICVGGFLAASMLCGTA
                     TSLPEMVAYRFLQGAFGAGMAPLGQALVMDSFPREKHGQVLSLWGVGAMLGPILGPTL
                     GGYLTEVLNWRWVFYINVPICAVALLGIWKTVPQVKARRDVPFDMFGFALLSLALGAV
                     QLMLDRGHSQNWFSSMEIVIEATVAGLCFYLFLVHMFTARAPFIEPRLFKDRNLVVGL
                     VLIAVVSMVMMAQMALLPSFLQQLMHIPVDTVGMMLMPRGLASMIGMVLTSRLIGRVD
                     QRAMMICGMVGFGLSTYEMSTINLYVDNFTIVRIGLMQGFSMAFVVSPLSIAAFSTLA
                     PDLRTESASMFALMRNIGGSVGVSVLFTRVAQVTQAEHARLGESITPFSDALPPAWQW
                     DTIAGAMSLDGEIVRQAASIAYVHTFLMMAVLALL"
BASE COUNT         4792 a        10616 c        10965 g         5258 t
ORIGIN      
        1 gcgtataggc taatccccta tcgagggttc gaatccctct ctctccgcca attccttgtt
       61 tttcgataac aacatcaagc ggttggaata gccgctgtgc gttgctgatg cgcctgcacg
      121 gatcctccag aatccccgat ctcctgcagg cgcgcctcac gatgtctcgg tagcgtttgc
      181 ctgacacaca gaggatgcgg ccttgagcgg ggaggcgcgc cgcgcgggct gccactcact
      241 gtcatcaacg cgtccgccag cggaaggcga gaaagcaggt tcaaacggga ttgctcctgc
      301 tccgccggca gattgacgtg ccattgacct gcgtcacatt ccggtcgtgg ttcacggtgc
      361 actctcgtgc ggcgcaggag gcatcacgac gtccgccagt gcgcgtaccg tgcccaggag
      421 atcgcccatg gcccctgttg ctccgcagcg attcgcgcag atcgcgctgc actggctcgt
      481 cgtcgtgatg atcgtcgcga tctacgtcag catggagtgc cgtggctggt tccccaagga
      541 cgttcgcccg acgatggtgc gggtcatgca ttattccttc ggtatcaccg tgctgctgct
      601 cgtgccctgg cgcctggctc tgcgggtggg ccgcccgatg ccgccgatca cgcctgcccc
      661 gcctcgctgg caggagcagg gtgcgcggat cgttcacctc tcgctctacc tgttcatgat
      721 cgcgatgccg ctgctggcgt ggctctcgta cagcctgcgc ggcaaaccgg tgtggttcta
      781 cggcttcgat ctgcccggcc tgctgccgcc ggcggacgcc gtgctccgca agcaactgcg
      841 cctgatgacc tggcgcaagc gcatcgccga ggcgggctac tggctgctcg gtttccatgc
      901 cgcggctgcg ctgctgcacc acttcgtgac cggtgacgac acgttgcggc ggatgtggtt
      961 caggaggacg tgacgacgac ccgccgggaa gagtccctga gccgcatcac gcagaggccg
     1021 ctttcccgaa gccgtcgaac gacgccagcc gttgcggaga ccccgacatg aagagtgccg
     1081 atgacctgag gatcttcgcc cgcacgatcc gctggctgcc cgcgatgctg agggcacggc
     1141 cgggcaagcg ctataccagc gccgacctgg tcgagagcag tgcgcggcgc ttcggcgatc
     1201 gcgtgttcgt cgagttcgag ggccgcacga cgagctacgc ggagttcaac cgctacgcga
     1261 accgcgttgc ccactgggcg atcgaccgcg gtctgcgcag gggcgacgtc gttgcgctga
     1321 tcatggagaa ccggccgcag tacctgggcg tctgggccgg gctggcgaag gtgggcgtca
     1381 cgacggcgct gatcaacacc cacctgggcg ggcacgccct ggaacatgtc atcgagtcgg
     1441 cggcctgccg cgtggtcgtg gtgggagacg agtgccttcc ggcgtatcgc gatatcgcct
     1501 cggcgcacgt gcgggctctg cccgtgtacc tcagcaggcg tgatggcgcg gccgtcgacc
     1561 ctgcggaggc gggttttgcc agcctcgacc gggagctggg cgcttgtccg accgacaatc
     1621 cgccggcctc ggtgcgggcg gggctgcagt cgggggaccc gctgttctac atctacacct
     1681 cgggcaccac cggcctgccc aaggccgcgc gcttcagcca cgcgcgcttc gtggccggcg
     1741 gcagctatca cctgtggtcc ggcttcggtg cgcgcgacgc gctctactgc tcgctgccgc
     1801 tctaccacac cgtgggcggc gtgatctgcg tgaacgcggt gctgctgacg ggggcgcggc
     1861 tcgtgctgcg gcgcggcttc agcgcgagca gattctggca ggacctcgcg gaatccggcg
     1921 cgacgacctt ccagtacatc ggcgagatgt gccgctacct gctcgcgcag ccgcccgggg
     1981 aatacgaccg gcgccaccgc atccgctacg ccgtcggcaa cggcctgcgg ccggacatct
     2041 gggcggagtt cacgcggcgc ttcaacgtgc cgcgcatggt cgagttctac ggcgccaccg
     2101 agtcgaacgt taccctggtg aacctccagg gccggcccgg ttccgtcggg cgcctgatgc
     2161 ccggcatcac ggcggcgctg gtgcgcttcg acgtcgagaa ggaggcgcac gtgcgcgatg
     2221 ccgacggtct ctgcgtggcg tgcgcggtcg gcgagcccgg cgagctgctc ggccggatcg
     2281 cgagcggcaa caagcccgag ctggtccgct tcgagggcta cacctcggcg gaggccacgc
     2341 gcgcgaagat cgtgcacgac gtgctccagc acggcgacgc gtggtttcgc agcggcgacc
     2401 tgctgaagcg cgacgctgac ggcttctatt acttcgtgga ccgcatcggc gattctttcc
     2461 gctggaaggg tgagaacgtc tcgacgcagg aagtcgagga atcgcttgcg gctttccccg
     2521 gcgtcgatgt cgccgtggtc tacggggtcg aggtgccggg cgcggaaggg cgtgcgggga
     2581 tggcggcgct cgcgctgtcg cgcgcgatcg atctgtcggg accggagttc taccaccacg
     2641 tcgagcgcct gcccgcctat gcgcgcccgg tgttcctgcg agtgatcggg gagatcgaga
     2701 tgaccggcac cttcaaggtg cgcaaggtcg aactgcagcg gcagggcttc gcgccgggcg
     2761 acgacgcgtc gcccgtctat ttccgcgacg atgccgccgg ctgttaccgc gtgctcgacg
     2821 acgcgacgta ccagcgcctg cgcgccggcg agctcaggct ctaggccgcc gcggcgtgac
     2881 cgtttgcctg cgtttggcgg cgcgcggtgg cgtgaccttg cgggccgggc cggcggcgcg
     2941 cttcgccacg cccttgccac gcttgacccg ccccacgtcc tctgcccgcc gcggctttgc
     3001 cctggtgtcg aaccgcaggt actccgcgag cgcctcgcgc aggccctcgg cgatccgcca
     3061 cgcatcgggc accaggtcga cgtcgtagat gatgccgaag tcgatgttgt cgacgtagct
     3121 gacgcaggtg aaattgaggc ccatgccgtt cgcgagcagc gacatcgggt acatcgcctc
     3181 gaggcgcgcg ccggccacgt acatcgggcg cgcgctgccc ttgacgttgg acacgatgaa
     3241 gttgcaccgc atcgtgtcga gcatcatcgc gggcgtcagt gccttgttca gcaccgcgag
     3301 ggcgaacggc ggcaacgagt tgatcagctc gtagatcgac gacctcccgc tgcgggccgc
     3361 ctgcttggcc gcaccggtct cggagtggat cgccatcagg cgttccaccg ggtccgcaag
     3421 ctcggtgtgc agggcaaggt tcaccgaagt gatcgcgttg ctggcggcgt cgtcgccctc
     3481 cttgcgcagg gagacagcga tgttcgcgcg cgccgattcc cgcggcagca cgccgaggtg
     3541 cagcaggtaa ttgcggatcg agctcgcggt cagcgcgagc acgacgtcgt tgatcgacac
     3601 ctcgaaacgg ttcttcacgc gcttgatgtc cgccagcggc agcgagaggc aggcgaagcc
     3661 gcgctccttg ccgatgacgc cgttgcaggg cagcgcgggt gcggctgcgg gccgcgcgcc
     3721 cgagcgcggc ttctccggct ggccgagcgc cgcgatggca gggcggagca tcgacagcgc
     3781 gtggcggccg gcgcgcgcag gagcttgcag cagattgccg accgtgcgcg cgtagagctg
     3841 cagttccgac ggcgcgccgc cggtcgctgc gccgaggaac tcgggcggga tgggccgcgg
     3901 gggcggatcg gcttcgaagt cgcagagagc ctcgccgatc ttctgcgcgc cctggccgtc
     3961 catcatgcag tggtgcagct tctgcagcag ggcgtagcgc ctgcccgcga ggccctcgat
     4021 gaaccagagt tcccacagcg gtttgctgcg gtcgaggcgg cggctgtaga ggtagccggc
     4081 gagctcagtc agcgcccgca tgtcgccggg cgcgggcaca gcgatgcggt ggatgtgccg
     4141 gtcgatgctg tactggtcgt cctcgaccca gtacggcagg tcgagaccga agggaacttc
     4201 ccgcagtttc cagcggaact gggggatcgg gtcgatgcgc tcgacgacga acgccttgag
     4261 cttttcgaag cagaaatcgc gcgacgcgcc ggcatcgagt atcaccagcc cggcggtgtg
     4321 ctggtagccg gtgtcggtct cggtgctgac gaagaggttg gcggactcgg agagctggag
     4381 catcgcgcgt tcctgcgggt gaacgggacg tggctgcgga ttctcgcaca caaggccgcg
     4441 tcatcgttga cgacgcagat caaggttttg cggattcccg ccgcgtgggc ggtgcgcggg
     4501 ttggactcgg cctgccaatc gcagtagatt ccagcgtgtc ccggtcaatc ccggccaccg
     4561 ggccgcggca aacggtgcgc tcatgaatcg tgaggcggtg agttccgata ttccggccgc
     4621 gtccatcttg gacctgccgc cgctggcgac cgtcagggtg ctcgacgcgg agatctgcta
     4681 ccgcgaagcg agcggtccgg cgaatgcgcc ggtcgtgctg ctggtgcacg gcatcctggc
     4741 gacatcgggg ctgaactgga tgcacgcctt cgcgccgctc agccggcact tccgcgtgat
     4801 tgcgcccgat ctctgcggcc atggcggtga ccgcttccac cgccgccggt tcacgctgga
     4861 ggactgcgcc gacgacatcg ccgcgctgat cgccgtgctg ggcctgaagc gcgtgatcgt
     4921 ggtcggctac tcgatgggcg gcatggtggc gcagtacgtg gcgcgccgtc acggcggcct
     4981 gatcagcggg ctggtgctct ccggtgtcga ctggggctcg ccgcaatacg gccgtgccac
     5041 gcagctcgtc ggtcccgcag tcatggaagc cgccgtgcgg ctggtgcacc tgcaggtctg
     5101 tctgctgcgt gcgccggtgg cgttgctgca acgcctgggc ggggcagggt ccgggcgacg
     5161 cgagccgggt ccgctgcgcg tgtcgatggg ggaattcacc ggtcataacc ctagggccat
     5221 cggtggcgcg gtgcgctcga ccatgcgctt tcgcagcctc gagtggctcc acgagatccg
     5281 cgtgccgacc accgtgctgg tcacgctgcg cgacaggctg ttcccgcagg cggagcagcg
     5341 gcggatggcc gaggccatcg acggggcacg ggtgagcgag ttcgacggcg ggcacgtcgc
     5401 ggtgagggag ccggcgtatg ccacggcgct gctcgaggcc tgccgcgagc tcagcgcgcg
     5461 catggcgcgg tccgcgcgct gaaggcgatg gcagcgcgcg cggaggtggc ggcatgtttc
     5521 ggctgagcgg acacgatgcg tatttcctgt accaggaaac gcagaccacg ccgatgcaca
     5581 cgctgaagat cctcatcggg cggcacaccg gcgggaccgc gccaggcctg cgcgagatca
     5641 agcagtcgat aggcgcatca ctggaccaga ttcccggatt caggcgccgc atcgtcccgg
     5701 tgccgctcgg cctgcaccat cccgtggtca tcgaggaccc cgatttcgac atcgacctgc
     5761 acgtgcaccg gctcgcggcg ccggaaccgg gcggtgccgc ggagctcgac gaggtgatcg
     5821 cgcaagtgtc ctcccacccg ctcgatcatt cgcgaccgct gtgggaattg tggatggtcg
     5881 agggcctcga gggcggtcgc gtcgccttcg tcgtgaaact gcaccacgcg atcgccgacg
     5941 gccaggcgtc ggtcaaccag ttcgagcgca tgctgggagc cggcgggcag gaaccggccc
     6001 ctgccatgcc ggtgccttgg cagcccgaag ccgtgccgtc cccgggcagg ctgttacgcg
     6061 atgcgctgcg cgaccagctc atggacatct ggcgcctccc ggcgctgatc gggcagacgg
     6121 tccgtaccct gcaccgcatc tacctgcgcc accgtgcgcg ggcgctcgac atcccgctgc
     6181 cgagcaaggt gccgccgacg cccttcaacc gctcgctgtg cgggcagcgc gatttcgcga
     6241 ccgtgcggtt cgcgctcgag gacatcaagg cgatcaagtc gggtctcggc ggcaccgtga
     6301 acgacgtggt gatggccatg gtggccggcg ctgtgcgcga atacctgctg gagcgcaagc
     6361 ggctgccggc gcagccgctg gtggcctcgg tgccggccgc cggcgtcgag caggccggcg
     6421 caaagcgcgt gttcggcaac acggcttccg gggtcaccgt ctacctgcgg accgacatcg
     6481 gcgaggcgcg cgcgcgctat gccgccacgc gcgaggcgat ggcggcgagc aaggagctgc
     6541 tggagctgct cgggcggaac accttccaca gctgggcgga ctacatacca ccgttgctgt
     6601 acaccgcgct ccagcgcctc aaggtgcatt tccgcctggc ggagcgccgc gccggggcga
     6661 tgaacctgat cgtgtccaac gtgcagggtc cgcgccaggg cgcgccgatg ccgggcttcg
     6721 acgtggagga actgatctcc ggcggcccgc tgctcgaggg cgtcgcgctc aacatcacgg
     6781 cctggagctt caacggcagg ctcaatttcg ggctggtggc ctgccgccgg gcggtgccgg
     6841 acctgcccgc cctggcccgg ctgctgcagc ggcagctggc ggcgctgcag gaactggcag
     6901 gcgcggggtc tgcaccatga gcgcgacacg gcgctggacg acgatcgagc cggcgtcggg
     6961 caggaagctc gcggtctcgg ccggcagcgg ccgcggcgtg cccttcgtct ggggccatgc
     7021 actgcagtgc agcatgcgcg ccgacgcggc gagcggcgtg ttcgactggt ccgggctcgc
     7081 gcccgcggtg cgcgtgatcc gctacgacgc gcgcgcgcat ggcgagtcgt gcagcgaggc
     7141 ggatccgcgc gggtgcgcgt gggagagcct ggcccgtgac atgtggcggg tcgccgagga
     7201 ctgcggcgcg gacttcccgg tgctcggcgg cgcgtcgatg ggatgcgcga ccgccgtgtg
     7261 ggcggcgatg cagcacccgc ggcgggtcgc cggcctggtg ctcgtcattc cgcccacggc
     7321 ctgggagtgg cgcccgcgac aggtgcgctc gtacctgatg atggcacggc tggtgcggtt
     7381 cgccggcagg ctgccgttcc gcgcggccag cgcgctcctc ggcgaccgcc cgcccgacgg
     7441 tccgctgaag gcgatcgcgc gggaggtcgt gcgcgatctc gcgacgcgcg atccgcgcga
     7501 cgccgaggtc gccttcaaag gtgccgcgct ctcggacctg ccgtcgccgg agcgctgcgc
     7561 gcgactgcgc atgcggacgc tcatcctcgc ctggcgcaac gatcccgcgc acccggtgga
     7621 ggtcgcgcag cggctcgcgg aggtgatgcc gaacgcgcgg ctcgagatcg cggagagcga
     7681 gcgcgacgtg cggcgctggc ccgatgcagt ggggaatttc ctggggaaag caggcgagcg
     7741 gcggcgcaag gcctgaggct gtgcgtgcgg ccgttgtgac ggcgggcgcg tccccggggg
     7801 agttccgcga agggcgccgg tggtcgtggg tgggcatgag cccgggttgc ttcagcaaga
     7861 aggtggcaat gagatgaatc ggattatccc gccgcgtttc gagggcacgt tccgcctgcc
     7921 ggacggccgc aagctgggtt acgcagagta cggccccgtc gacggcaagc ccatcctgtg
     7981 gtttcacggc acgccgggag cgcggcgcca gatcgcgccg gaggcgaggc gtctggccag
     8041 ccgccgcaaa gtgcggctga tagcggtgga gcggccgggc atcggcgatt ccacgtccca
     8101 cagctacggc tccctggccg attgggccga ggacatccgg gtcttttgcg acgccaaggg
     8161 aataaaggag ttcgcggtct gcggcctttc cggtggcggc ccctatgcgc tggcctgtgc
     8221 gttctatctg caggggcggg tagtggcggc gaccattctc ggcggcgttg cgcccgcgcg
     8281 cggcgacgat gcggtcgagg gtggcgtgtc cacgctggca cggcgcctgt cgccggtggt
     8341 caggcacctg cgggtgccgg tcaaccgcct gatgctgcgc ctgattcggc gcctggagcc
     8401 gcacgccgac aaggcgatgt ccctgttcgc gcggttcatg ccgcccggag acaaggtgat
     8461 gttcgaggat cctgaattgc aggccgtgtt gacggacgac ctgctgctgg gaagccggac
     8521 acaaatgcag gcgatgatcc acgacgtggt gctgttcgcc agggactggg gttttccgct
     8581 cggcaagata gaaacgcccg tctacctgct ctatggcaac gccgacaaca tcgtgccggt
     8641 ggaacacggc aggcacatgg ccgtgcgcat tcccaatgcc gtgttccgcg agcgtgaagg
     8701 tgaaggccac atgggcggac tgggcgcctc ggaagagatc ttcaacgacc tgctgtccca
     8761 ctggccgcgg cgtcggcgcg agtagcagga acggggagac gacaggtccg ctggctgcct
     8821 ggcggtagcc ttcgttccgc gctgctcagc gtatgccgcg accgcccatg cgcgccggcg
     8881 tcatctccgc gtccggcagg aagctcacgc cgcgacggtc gtagcagtga ctgcacagat
     8941 gggcctgcat caccgcgtac tggccgcgga ccaggtcgta ggcctgcacg gattccgcca
     9001 tgaagtagcc gccgtgggcg tagtcggcca gaccgttgcc gaacaccacc cggtagagct
     9061 tgccggagtg gtcccaggcc tcgtaggcga gcgcgaccca ggagtcctca tcgaggtaga
     9121 agcgcttttt cgcggccatg tgccgcgcgc cgggcaaggg cgtcgcctcg accacccata
     9181 cgcgccgcag ctcgaagcgc cactgctccg gtttgacgtg atgcgggcct aacaactgcg
     9241 ccgggtcacc gcgcagcagg tgcatgcggt agttgttgca gggcaggtac atcagcttcc
     9301 tgcccgtcag ccggaagtcg aagcggtcca tgcgccccgc aaacatctgc acctcgtcgt
     9361 agaacagcgc accgccgatg tgtgctatcg gcgtgtcgta ggagaactcc ggcgccatgc
     9421 gcacgcgccg ctgcccggcc tgataggtcc acgcgcgctg gtcgtccacg tcgaattgca
     9481 gcggatacca caccagcgtc tgagaaccgg cttcgcgcac gggctcgatg ttggtggcga
     9541 atccacggag gtagtagcgt ccggcgccct cgtacggttt ctgctccggg ttgaaatagg
     9601 ggttgtcgcc gatcgcgctg ttgatgacga gcagcgagat cgcgccgttg gcgttcacca
     9661 ggtaggaatc cgcggtcagt ttcccggtcg ggatattgaa cagcaggtgg ttccacatgg
     9721 cctggtagcc gttttgcgga accggaaagg gcgtgccgcc ccagcagccg tagacaccga
     9781 cgccgttcgc gctggtcctg cactcgggat tgcgcgcgtt ggccagcgat ttctgctgga
     9841 tccgttccgg cagccagaag ccgcggcgcg tggggtagat gtccatgcgg aagctcggat
     9901 agcggcgcat cagctccaac tgccctgcca tcagccggtc ggtgtactgc tccatgttcg
     9961 tcgcgtcgat cgagagcagc ggcttgtcgt cggggtaggg gtccggccag cgaccgctct
    10021 tctcgtcgaa tcccggcggc gcctcgatgc cgcccgcgta ggccggatag ctgacgtccg
    10081 gcgagccggc aatctctgca cctcatggcg tgagggtggt gccgagttgc gcgagctcgt
    10141 cttccgacac cgctgcgcac gctgccgcgc agaccagtaa cggtgccacg aggcccacga
    10201 cagcgtgggt caacttgcga atgtgcatgg ccttttttcc cggttctcgt gaacactgtt
    10261 atcggttatt gctgttccgc cttgcggtgg ctgcgcccga cgaaggtcgg cggatccgcg
    10321 atgaacgctt gcgccgaagc cttgctggtg gcaattgcat cgagcaggtc gtctatcgtg
    10381 ttcatcgctc cttccgttcc tgcgattccc gcaaccgcgg gactcggggt cgaggcaacc
    10441 gcggtgtccc gtagctcagg gcgtggcggc ggccgtcttc cgcaccgtgg cgcgtttgcg
    10501 cttgcgcatg ccgggcttgc cggtgccgac cttgccgcgg cgggcgcccg tgccggtcag
    10561 cgccacgtac tctgccagcg cggtgtgcag gccatcgatg attcgccacg ggtcgggaac
    10621 gaggttcggt tcgatcgcga cgccgaaatg gatctcgtcg gcgtagctca ggcaggtgat
    10681 gttcagccct ccgcccggtg ccaggatgga catcgggtag atggcgctca cgcgcgcgcc
    10741 gccgacatac atcggtgccg cgctgccgcg catgttcgaa acgatcacgt tgacgcccat
    10801 cagtcgcgac acctggtcgg ccggcgccat gcgcatgatg gtgccgacca gcacgggcgg
    10861 cagcgcttgc acgaactcca gcactccctt gccgccgtgg tgggcttcct ccttagcggc
    10921 aactgcctcc gcggcaatcg cccgcaggcg ttgcaccggg tctgcgaggt ccgtggccaa
    10981 ggtcaccgag gcggtcgtga cccggttgct gaattcgtcg tccgcatcag tgcgcagcga
    11041 tacggcaatc gagctgcgca gcgcctcgtc cggcagcgcc gcccgcgcga gcaggtaatc
    11101 gcgcaggctg ccgccgacga cggccagcac cacgtcgttc acggtgacgt cgaagtgcct
    11161 cttgacggtc ttgatgtcgc gcagcggcac tgaaccgaag acaaaacccc tgtcgccggt
    11221 gatgtcggca ttgaatatcg cggtgggggc ggtcggtctc ttcttcgacg catcgtccgt
    11281 cgcgctgaag cgcttcgcca ggctgcgcca ggccacatcg taaagcccgc ggccgatctg
    11341 catcggcatg ccgtagaggt gtctcgccgc attgagcgac agctgccatt gttccggcac
    11401 gtcccccggc agtgcctccg cggtggccct gtcgacgggg cgcggcgccg cgtccggctc
    11461 caggtcccac atgtgatcca tctgcttcat ggcgccggta ccgtccatca tgcaatggtg
    11521 catcttctgc gccaccgcga agcggccatc cgcgagcccc tcgatgaacc agatctccca
    11581 caggggcctg ctgcggtcga tgtgcctgct gtagaggtag gccacggtct cgccgagcgc
    11641 cctctggtct cccggcgagg gcaccgcgat gcgccggatg tggtggtcga agctgaagtt
    11701 ctcgtccgct acccagtagg gcaggtccag ccccagcggc acttcgtgca gcttccagcg
    11761 gaattgcggc acgctgctga gccgcgtctc gatgtggcgc cgcagcgcgt cgaagccgaa
    11821 gccgaagccc ggcctgccgc tgctgtccag caggatgagc cccatcgtgt gctggtagac
    11881 gtgtgcgttt tcgccgccga cgaacatcac gtcctggggc ttcagttgtc tcggctttcg
    11941 catgctcgcc tcccggcgcg ggagtgaatc ccgacgcgtg cagccatgcc tggcctgcgc
    12001 gccgcttcgg ttcgacgaca ccgtcgtctg ctgcttcttt tgccgacgca gcttgcccgg
    12061 aatcgccgcg taccggtagc cggtcggcag gattcgttga tcagggtcaa gcgggcactt
    12121 cgaaatcccg gggctcacac gcagcggtcg gtcgtcgggc ggtcgcggac acgggcagtg
    12181 ccggtctgcc ttccctgcca cgtcgcgcgg cgatagcatg cgcaccaccg ccctacgggt
    12241 tgcaccgtga tccgaaatcc ggccgcgcca gttgccctca cgccgggcac ggcggcatag
    12301 atcgccaggg agtcgagcca tgagcgtcac gaaccaccag tcgggaacga acgtccacga
    12361 ggttgccgac gggatctaca gcatcaacac gccggtgcag ctcgagggcg gcgtggggtt
    12421 ctcgttcaac cagcatctgg tcctcgatga cgagcccctg ctgttccaca ccggtccccg
    12481 caggatgttc gcgctggtgc gcgaggccgt ggcgcacgtg atgccggtcg aaagactccg
    12541 ctaccttgcc ttttcgcacg tggaggccga cgaatgcggc gcgctcaacg agcgccacgc
    12601 tgctgtgcgg cgacctgttc acgcagggcg gcaccgacct gccgccgctc accgggtcgg
    12661 acatgtggat ttccgagaag atcgcgaaag atcctcggat ctcggacggt aaaggcgcca
    12721 cttacatcta tcgccatggc aggccgcgaa aataattggg caccggcatc tgcgcgccaa
    12781 tgcgcagcta aacacttgat tttttgctgc aacgggcagt tcggcaaaca tgcgttaggc
    12841 aaactcaatg tttgcggggt gcgtgttacg tcgatgatta atatagctat ttcccttcgt
    12901 tcgcatcgcg caatacttgc ctaaccatga cgcctagagt cctgctcgac gggttgcgtc
    12961 gaagcccgcg ttactcaggc gcgctgccag cccgcgccac ccgcagggcg gccgggctgg
    13021 agcttggtcg tcgccgtcgt cgtttcaggg cgcgaccacc gtttgcggct gcgtgctgac
    13081 gaaggtgtat tcggtcacga agcccgatgc gccctcgccg ggtgccaccg ccgagcccgc
    13141 gctgacgaat ttcagttccc agccgctgtt cagcagatcc gctactcccg tggcgcagtt
    13201 gtcaccgagg gtcacgggca gctgcaccag ccctgcgtcc tgctccgagg cgctggcggc
    13261 aaccaccacc acgctgccgg cgacggaatc acagcgcagc accgccacgg aggccagtgt
    13321 cggcgggatc acggggcacg cgccgagggc gtcgccgtgg ttggtgtgcg ccctgaccgc
    13381 gctctgcgag atgctcagcg tttcagtgcc cttgtggcaa acgttgacct tctcggtcgc
    13441 ggccagcgcg ggctgggccg caactgcggt gagcagggcg atccgcagga tgtgtttcaa
    13501 gttctttgct ccgttggttt ccagttgaag gagtcaaaca cgtaccatgg gccatggaac
    13561 tcatatcatg aaccggttca tgaaacagga tgaacgcggg ctgtatctgg acttcgcgcg
    13621 attccgtcgt ggctgaagga ctgacggcac aaatgatcag cagcgctatg ccaacccgcc
    13681 tgtcgcgtca cttcgccgtt gcggtgctgc ttgcagcggc ttgcctcgtt gccggctgtg
    13741 cggcaacgcg acagccggtc gatacggacg cgacgcatcg catcgaccct ggtgcgcgct
    13801 ggcagatcaa cgccgatcga tggaacgcga ccttcgagat gtcaccgcac gccgcgtacg
    13861 gctggtcggg cggtgatgcc gcgatttcgc tggcgctgcc ttattgcccc gaatgccccg
    13921 ccggcagcca ccatcgccgg ctgtggctgt tcggtgattc tgccttcagc acggtggata
    13981 ccgaaggcta ccgcgtgaag gccgatgggg agcgtggcgc cgattacacg ttcggcaacg
    14041 ttgccgcgat caccgcgcat gccgcgccct ctgccccggg ggccgatacc gtcatttttg
    14101 attacggcgc caggggcttc aggtcgaact cgtggatgcc gctcctgctc gatattcggc
    14161 aactgcccga tatggatgac ctgcgtattg ctgccgcggg ttatgcggac ctcgggttcg
    14221 cccgcgcgcc gcagccggtg gcaggcgtgt actcgttggt ggaactcgcg cccgaggatc
    14281 tgcgttcgga cctggtggcg gtaaacgaat attacgatcc tgccagtcgc gccagtgact
    14341 acatcacgcc ggatgttgcg gtgcacggat tgcctggcac ctacgagtat cggcgcgtgt
    14401 cgttctacat ccatcgctcg ccgcagcaag gcaccgtgcc gctctatcgt tatcgtttcg
    14461 ccgcgcatgg cgacgtcgtg ctgagcacgt ccgcccgtgc gccggttggc gcgggatctg
    14521 gcgccggcca gttgctcggc cacgtctacg cggcaggatc cgcgccacgg aacgtgcatg
    14581 cactggtgga atattcgcgc gtcgcgaccg cgcatcacgc tgcgcagcat gcctacacca
    14641 cccgccccaa cgatgctggc aaggacatcc tgatgtggcc caatcgcggg ctggtgatcg
    14701 gcaatgacct ggtccaggtc ttttcgccgg tcacgccgct ggatctggcg ggcatggtgc
    14761 cgtgggacga catcgacagg aatgtggtca gcatcgttcg cggtgtcgat cggccgtatg
    14821 cgcagtgggg caagacccct cagggcgact ggctggtgtc gccgcggcag gttgcgctgc
    14881 gccactccga tgcgcgcacc agttggggcc tcttcctgct gaaggacgag cacttcgagc
    14941 gcaatcgcca tgtctatgtc tacggcaggc gggaaggaaa gctggtgctc gcgcgggtcg
    15001 cgtgccgcga cgccaatgat ttcgtgcgct tcgggaactg gcgtttctac agtgacggac
    15061 gctgggtcga gtcgcaggaa gatgccacgc cgatcgccga ctacgtggcg acggatttct
    15121 ccatccacga aagccctgcc ggtcatttcg tgctgatcca gtccccgccc ggcctggtgc
    15181 ccgcgattgc cgttgccgtc gcgtcatcgc cggcaggccc gtttcgcgct ggccccgacg
    15241 tcgacctgtc gggctaccgc aacgaggccg atcgccatcg gcgctacagc tattacgccg
    15301 tcagcgccca tggcgggctt tcggttggcg gggacagggg tggcctcctg atctcgtacg
    15361 tgcgctcctg tgccttcagc gcggatgccg cctgcacggg caaggatgcc gatgagaacc
    15421 gggcggatgt ctatgtgccg cgcttcatcg atctgccatg gcgcgtgatc gatccctgac
    15481 gacggtcggt ttgccgcgga tcgcccggat ccactggcca ttgagggccg caatgatccg
    15541 gtcgagcatc gccggagtca gtccgggtcc catgccggcg agtccgcgca gcggcatgct
    15601 ggccacgacg ctttccatca gctcgcgcag ggaaccgtcg tcgtcgcccg ggatcatcct
    15661 gtccatctgg cggcgcatca gtgcggccag cacccggccg aggcggctcg ccgacagctc
    15721 ggtcacggtg gacgtgcggc tgaagggtcg cggaccctcg gctgccggga tcggtcgtcc
    15781 cagcaacgcc gcgaacccgg cgtcggaggc gatcggggcg gccagcggcg cacccggcgt
    15841 gatggtgtcg gtcgactcga catcgatcat cacccggtcg tggatctcga cgctcgatgt
    15901 ccccgcgagc acctcgtact ggccgcctgc caccacccag cggcccgcgg cgctgtccca
    15961 cagcgcgaac acgcgcggct ccagcaccac atcgagctgc gcggtcgccc cggcctcgag
    16021 gcgcagccgg gcgaaaccgg ccagttccct gtcggggcgc ggcagcaccg gtgcgacggc
    16081 ccgcaggtag agctggacca cggtagtccc cgctcgcgcg cccgtgttgg tgatggtgag
    16141 cgtcgcggtg cgggcagtgc cctcgccgcg aacccggaga gcgccgaggg accaggtcgt
    16201 gtatcccaga ccgtgcccga acgggaaacg ggccggcacc ccgaaagtgt ggtggaagcg
    16261 gtagccgatc cactggcatt cgcggtactg cacctggcgc ggaagtcccg gaaagtttgc
    16321 gtcggcgggc aacccggatg tcgcgacagg caggctttcg gccagccgcc cgccgggttc
    16381 ggccttgccg agcagcacct cggccagtgc cgagccggcg gcctggccgc cgaggtacga
    16441 ttcgaccagg gccgccggca gcccggccca gtccagctcg aggctgcccc cgttcatcag
    16501 gaccacgacc gtgcgcgggt tggcgcggca gacgctggcg acgagctcgt ccatggcgcg
    16561 cgggagccgc gtggtgggcc ggtcccgggt ctcggtctcc aggcgcggcg gggtgcccac
    16621 gacgagcacg acgcgccggg ccgccgtggc aacggccatg gcctgcgcca gcagttgcgc
    16681 gctggtctcg cccgttgccg ggtcatacgc cggggcgtag ggcagctcga tgcccagggc
    16741 atcgcgcagc gtgtccagcc gcgcggggtt cacccgcgac gagccggcgc cctggtatcg
    16801 gggctcgacg gcgaatgccc cgatcagcgc gacgcccgcg gtgtcgccca atggcaggaa
    16861 cccgtcgttg ctgagcagca cggttccggc ggcagcggca cggcgcgcca ggtcgtgatg
    16921 ggcctcgaaa tccaccggct ccaccgcgcg ctcgcgcagg gcatctgcgc gcagcgccag
    16981 cgcggccacc cgggtggccg aggcgcgcag gtcggcttcg gcgagcgccc ccgacgcgag
    17041 cgccgcgacg atctccggag tgaatgcgcc gccgttgccc ggcatctcca ggtccatccc
    17101 ggcccgcagc gcggcgacgc ggtcattggt gccgccccag tcggagacga ccaggccgcc
    17161 gaagccccat tcaccgcgca gcacgtcgcg cagcagccac gcgttgtcgc tgcaccaggt
    17221 gccgttgatg cggttgtaag cggtcatgat ggccgccggc cggctgcgct cgaccacgat
    17281 ctcgaagccg cgcaggtagt actcgcgcag cgtgcgctcg tcgatcagcg catcgacgat
    17341 catgcgcgag ctctcctggt tgttggccgc gaagtgcttc gcgcaggccc cgaccccctc
    17401 gctctggata ccattcacga tggcggcagc gaggtatccc gccagtacgg gatcctccga
    17461 gaagtactcg aagttgcgtc cgccgcgcgg gtggcgcttc atgttcagcc ccgggcccag
    17521 cagcatcgcc acgtcttcgg cacggcattc gcgcccgagc gcgcgcccga tctcgtgcaa
    17581 cagttcgacg tcccagctcg aggccagcac ggtggcaacg gggaaacacg tggcgggtac
    17641 cgagttggca agcccgagat gatcggagtc cccgacctgc ttgcgcagcc cgtgggggcc
    17701 gtcggtcatc gtcgccgacg ccaccaggcc gggcagctca cgggtgctgt acatgtcgcg
    17761 gccgccgacc agcgcgatgc actgcgcggc agtgagcccg gcgacgatcg cggcggcgcg
    17821 cgtggtcagg gtgtcgtcgt acattcggcc gatctctccg gatcaacgtc gtcattgcga
    17881 aaataacacg cgcctgtccg gccctggcga tgcgaacggg gatttcttcg ccagtggatg
    17941 cgtgaggccg tgtgcttgcg ggtgtgtcat gcaattgccg tttgtgcgtg ctaccgttgg
    18001 cgcgccgcct cgaagcaggc gcagacagca atattgcacg agaacttccg gatgtccctg
    18061 ctgaatcccg tgccgccact ggccgccctg ttcctcaccc tggtgctcgc cgctcaggcc
    18121 ccgtgcgttc acgccacgac gatcgtccag cgcgacatcc gcgaagtcgc cgagggagcc
    18181 acgctggtcg tcgagggcac ggtcaccgcg gtcgagtcgg cgccggtgat gcagggcagg
    18241 gcgattgcca gcacggtgac gatagcgatc gaagaggtgc tcaagggcag ctggagcgcg
    18301 gagagcatcg atctgcaata tctcggtggc agccacgacg gccgcagcat gagcgtcggg
    18361 gagatggaaa tacccgcggt gggtgagcac ggcatctatt tcatccgcga tccgcgcagg
    18421 cagttcgtgc atcccttgaa gggttgggag caggggcact tcatcgtcgt cacggacggc
    18481 ccccaaggcg aggaaacggt ccgggcgggc aacggcaagg cggtggtcgg cttgcctgca
    18541 gacgctgcac cgccggcgca gggccaggtg atcggcaagc gtgtcgccgc ggggctgcgc
    18601 accgcggacg gcccggatga acacgccctg tcggcggcgg cgttccgcga ctggatcagg
    18661 agccgatgag gccggggaga acagcacggt gaggcagcat gcactggtca tcgcgttggg
    18721 cgcggcgttg tgcgccccgc ccggcgcgga tgcttacgag atcgggtttc cctaccacaa
    18781 ccccgtcatc gacatgcagg tcgatatcgc cggcagcagc ccgtccggga cgcgctggaa
    18841 tgccgccttt gccgaggcgg cgcgcagatg gaacagcgac gccggtgttc gaatcgacgt
    18901 ggtgaatcgc tattccgatc cgtgtgccgg cgttgtgccg ctgactgtca acgatcccct
    18961 gaacggcgtg gcattgcgcg acgatctgtg cggtttcgcc ttcgacgagg acaccctcgc
    19021 cgtcacgctg cgcatgttcc agctgtattc cgaggtcacg atggtcgagg cggacatcgt
    19081 gttcaacgac agcgtcgcct gggacgtgta cgacgggccg ctgcgcagcg ggcggccgga
    19141 tttccggcgc gtcgccacgc acgagctcgg ccatgtgctc ggcctggaac acgaggacga
    19201 ctcgcccgcg ctgatgaatt ccgccatctc gggcatctac gcgcccaacg acgacgaccg
    19261 caacggcgtg tcggcgttgt acggatcgct cgccgccggc gacggcaaca tcgagctcat
    19321 gatcgaggag ccggcgcagg gatcgacggt cgccggcgtc ggcaacatcc gcggctgggc
    19381 gctcggggat tccaacatcc tgtccgtcga gctctacatc gatggcaggt tcatcgccga
    19441 cattcctcac ggcggcacgc gcaacgacgt cagggacgcc ttcccggtgc gtcccggctc
    19501 ggccgaggcc ggctactcga tggccttcgc ctggagcctg ctgacgccgg gagtacacca
    19561 gctacgcatt cgcgcgatcg atttcgaggg ccgcgcggcc gaggccaccg gcagcttcac
    19621 ggtcaatgca ttcggcaccg agttcatcgg cgaaccggaa aaggtatcgg tcaacggtgg
    19681 tgtggagaaa ctcgacgcgc gcacgatccg cctgaatggc gtgcaggccg acggcttgct
    19741 ctacgacgtc aagctgcagt ggcagccggc gagccagaaa ttcgagatcg tcgagatcga
    19801 gccggcagac tgaagccgcg gcgacgattt cggcgggttc aattttcggc ctgctccgcc
    19861 gccagccact cctggcggcg catcgcctcg gcgcgcacat cctcgatttc cttcagcacc
    19921 gcctcgacgt cggcaagttc ggtgggttgc gcgaactcgc cggtgagcgg cgtgtcgggc
    19981 tcgagcgtgc cggcctcgta gtgggcccag atctcgttgc cgtaatcggt gccgcgcaat
    20041 tgcggggcga actggccgag gtaatcggcc aggttcgcga cgtcgcgctc cagcatgcgc
    20101 ttggcgtggt tgttgcgcgc ggcatccacg gcctgcggca ggtcgatgat cacggggcca
    20161 tcgtcgtcga ccaggatgtt gaactccgac aggtcgccgt gcacgatgcc ggcgcacagc
    20221 atgcgcacga cctccccgat cagcgtgtgg tggaatgccg cggcctgctc cggcgcgagc
    20281 tccacctcgg ccagtcgcgg cgccacattg ccctcgccgt cggccaccag ctccatcagc
    20341 agcacgccgt cgaagaagcc gtagggcttc ggcacgcgca cgccggcggc agcgagcttg
    20401 tagagcgcgt ccacctcggc gctctgccac gccgattcct tttccttgcg cccgaagcgc
    20461 gagcccttct ccatggcgcg ggcgcgccgg ctgctcttaa cgcgacggcc ttcctggtac
    20521 tccaccgcct ggcggaagct gcgcttgtcg gattccttgt agatcttggc gcagcggatt
    20581 tcccggccgc agcgcacgag gtagaccgtg gcctccttgc cgctcttgag ctggcggatg
    20641 acgtcgtcga cgagcccgtc ctcgatgagg ggttcgaggg cgcgcggaat cttgatggtg
    20701 gcgatgggcg tggcttccgg agttgcgtgc ggcaggcagt atacggaccc gcccgcatgg
    20761 cgtacccgtg aatatggcca cccggcgcgc ggcgaaaatg ccgtttcgcc acgcgctccg
    20821 gggccgcgcg ccgggcgcac gggtgcgcag gcttcagtat tcccccggca gatgacatgc
    20881 taggccacgc gctcgccacg gcggcgccgg cagacaaacc atcgcgaggc aagcctcatg
    20941 acgcacaagc gcaccatcgt ggacctgatc ggcaacacgc cgctgctgta tctgcggcag
    21001 gtgtcggaac tgaccggctg cgatatctac ggcaaggcgg agttcctcaa tccgggcggc
    21061 tcggtcaagg accgcaccgc gctcggcatc gtgcgcgacg ccgaggcgcg cggcgtgctg
    21121 cgccccggcg gggtcatcgt cgagggtacg gcgggcaaca ccggcatcgg gctcacgctc
    21181 gtggccaacg cgctcggtta cgagagcgtg atcgtgatgc cgcagagcgc cagccagcaa
    21241 aagatcgacc agctggaaat gctcggcgct gagctcatcc tggtgcccgc ctgcccctac
    21301 agcagcccgg ggcattacat ccatgtcgcc gagcgcgtgg ccgaggggcg cagccaccag
    21361 gaagcccacg gtgcggtctg ggcgcggcag ttcgacaacc cggccaatgc acgcgtgcac
    21421 tttgacacca ccggcacaga aatctgggcg cagaccgagg gcaaggtcga tgccttcatc
    21481 tgcgccgtgg gaaccggcgg cacgctcgcg ggcgtggcgg cggcactgcg cgaacgcaat
    21541 cccgccgtga agatcggcat cgcggatcct cacggttccg ctctctacag ctatttcacg
    21601 aagggcgaac tcgccgcgca cggcgaatcc atcgccgagg gcgtgggtgt gagcttcgtg
    21661 cccggcaacc tgcagggcac cgcggtggat cttgccttcg cggtggacga cgataccgcg
    21721 ctgccgttcg tctacggact gctgaagcac gagggcgtca gcgtcgggcc gtcgtccggg
    21781 atcaacatcg cgggcgcggt ggcgatggcg cgcgaactgg gtccgggcca caccatcgtc
    21841 accatcctct gcgactccgg cagccgttat ctcgatcagc tatacaacgc ggagttcctg
    21901 tggagccgca aactgccggt gcccgactgg atcgtggcct gagagccgct catccggctg
    21961 gcggcagccc gagcccgcgc gcgagtgcgg cacagctgtc cgcccactgg cgccggctga
    22021 tcgccgcgtc ggtgatcagc gaggagccgt ggaaggtgcc ggggtaggcg tgcaactcca
    22081 ccgacacgcc ggcctgcaac aggcgctggg cgtagtgcag cccctcgtcg cgcagcggat
    22141 cgaattccat cacggtgacg caggccggcg gcaggccggc gagatcgctt gcgcgcgccg
    22201 gtgccgcgtg cgacggcacg gcggcgctgc cgggcgccag ttcgcccagg tagtagcgcc
    22261 agctgagttc cgctgccggc aggttccaca gtggcgtatc ggtgaaggcg cgcatgctcg
    22321 gcgtctgcag gcggtcatcg agctccggta tgccgagaaa ctggaagcag ggctgcgggc
    22381 cgccgcggtc gcggcgcagc aggcacagtg ccgccgcgag cccgccgccc gcgctctggc
    22441 cgaacacgcc gaggcgcgcg acatcgatgc cgagttcagc cgcgttcgcg acgctccaca
    22501 ggaaggccgc ctcgcaatcg tgcaggccgg ccgggtaggg gtgttccggc gcgaggcgat
    22561 agccgacgga gacgagcacg atgccgagcg agctcgcgag cgcgatggca ttgccgtgct
    22621 cggtctcgag gctgcccacc acgaaaccgc cgccgtggat gtacagcagc cccgccacgg
    22681 cgccctgctg cgcgagtggc cggtagatgc gcaccggcac ggggccgtcc ggtccctcga
    22741 tcgtgcggtt ctcgatgtgc acggcggaag tatcgggcgc cggcatctgc gccgacatgg
    22801 ccgtgatggt ggcgcgcgca accgcgacgt cggagaaatc gagctgtggc aggaagggaa
    22861 cggcgggtgc gagctcggga tcgaagcggt aactcatgtg gcgggtcctt ggttggtgtc
    22921 ggtcgatcag ggattgatgc gccgggcgca ggccccaccg gcgcgccgcc cgaagaaggt
    22981 cgcctcgccg aggcatgtgc cgctcgagta gccgcgcgca tcctgcgcga tattccaggc
    23041 cgtggcgccg gcggcataaa ggcccggaat cgcgccgccg gaatcgtcgc gcacttcgcc
    23101 gtcggccgac acgctgagcc cgcccagcga gaagccaccg tatttcgcgc gtcccaccga
    23161 cagatcgaag gcggcccagg ggccctggtc gagcggctgc agccagtcct tgtacttgtg
    23221 gaacgcgggg tcctcgccgc gcgcggcatg cgcgttgtag tcggccaggg tgcgcaccag
    23281 cgagccggcg ggcagttcga gtcgcgcttc catttccgcg accgtctcga agccgtcgac
    23341 caggcgctgc tcgaagaaat cgtagggcgg gtagccgaag gtggccgagt cgaggaccag
    23401 gtacacgatg ccctgcggct gctcgacgat gaagccggcg ctgcgtccgt ggtaggagtc
    23461 ctccgccacg aagcgctcgc cctgcgcgtt cacgaggatg cccttgatca ggctctcggg
    23521 cggatagaac ggcgaggtca ggaaggcacg gtccagcccg caggcagcgg cgccggccga
    23581 caggccgagc aggatgccgg cgccgtcgtc gccgggcgaa ccctggcgga tcaggtcacg
    23641 cggcatccac gccagctcgt gggcgaacat gtcctcgttc atgatgtagc cgccggcggc
    23701 cagcaccacg ccgccgcgcg ccgcgatgta cagctccgag ccatcgcaga gggcccgcgc
    23761 gccgacgatg cgtccgtcgt catcggcgac cagctgcgtc acccgcgtat cgaactgcgt
    23821 cgcgacgcca gcctgcagcg ccgcctgcgc gagctgctgc atcagcaatc cgccgccgcc
    23881 gtccggtccg tcgaaggcga ccttgtgccc gcgcggcgcg ggctgcgcca ggtcgcgata
    23941 gggccagacc ttctcgttgc cgctccagat caggcattcc ctgccgggct gcatcgtggc
    24001 cttgcccggg tagtagctgc ggtcgaaggg cacgccgtgc gcctcgagcc agtggaagtg
    24061 cgccacgctg tgctcgcagt agagtcgcag cttcagcgga tccggatcgg gcgtgagcgc
    24121 ctccaggtac ttgtacattt cttccgggga atcggcgaag ccgcaggcct gctgcaccgg
    24181 cgttccaccg cccagataca ggtgtccgcc cgcgatgccc gaggtgccgc ccgcgccgct
    24241 cgtgcgttcg agcaccagca ccgcggcgcc gctctcgcgc gccgcgatcg ccgcgctggc
    24301 gccggccgcg ccatagccga tcaccagcac atcggtgctg cgcgtccatt gccgcacgtc
    24361 ggcggcacgc agcagttctg gcgcgttgcc gcgcaggctc gctcggattt cgctcatcgg
    24421 ttctcccgtt atcgtgatgt tccgttgccg gccgccgggc gggcaggcgg ccaagggtaa
    24481 ccagcttggc gcgggcattt caatgcgcga agcccgctgc atcggcgaat tgcacgagcg
    24541 cgcggtgttg cggcttcggc gatgactgcc ggcgcattgc ggcgctgtac cgcaacgagc
    24601 agacgcgcgc cctggcgggc gccgtgggaa gtcgtggagg cgttcatccg cgccatggcc
    24661 ccgcgctgag gcgcctcatc cgatcgaacc atgacctgcg gctgaaatcc ggttagcatc
    24721 cgcggctctg cgaatgacgc gctcgcgcga gcgccagtga aatgccgggg aaacgcatga
    24781 cggacaaggc tgcgcaacgc ctgctctcgg gcagggcatg ggacgatttc tgcgaacagg
    24841 tacgcctggc gggccacatg gtcgatcgct tcggcgactc cgccgacgcg ctcgaccgcg
    24901 ctgagtggta tcgcttcgtg acccggctga tgcgcaacgg cttcgagcgt ttcatggaga
    24961 actgcgagcc cgagcgcccg cgcctgcgcg atgcgccgtg gcgccagggc atcaacttcc
    25021 agtcgccgga ccaggaccac ctgctcgcgg agttcgtcga tggcagccac gactaccgca
    25081 tcaccggcaa ccgcggcacg ctgccgtatt tcgtgatggc ggcgtggagc gcggggcagc
    25141 ccgctgatat cggcgcgcgc gactgggcgc cgcgcggcgt ggagggactc gcggagttcg
    25201 accctgcgat gctgcgcacc accggcttcc tgcagagcga cgcgatccat ttcgatgccg
    25261 agggcaattt cgaggtgatc gcgagccgcg agaagccggc cgacggcagc gactggctgc
    25321 ctatcgcgcc agactgcgtc ggtatcctgg tgcgtgcggt ctatcaccgg cgcgagcaga
    25381 cgcgagcgcc ggtgatgcgc atcgagcgca tggacggggc gctgccgcgc ccgctacgcc
    25441 cggacgaagt gagcgcggga ctcgcgaagt ccgcgcagat ggtgctgggc tacgccgagc
    25501 tggtgcgcag ctggtggcag gacaacctcg gcgcgcgccc caaccgcatc cgcttcagcc
    25561 gcgcggtgta tttgtccaac ggcggcgtgc ccgatcgcca ccacggcttc ggtacctggg
    25621 agcgcgcggg cgacgaggcg ctggtgctga gcttcgtgcc cacgccctgc gactactgga
    25681 tcttccagct gtgcaacatc tggcaggaga acctcgacaa ctacgaggac ggcgacggat
    25741 acgtgcagaa gtacaccgct gcgtacgaac ccgacggctc ggtgcgcatc gtcgtcgccg
    25801 aggaggatcc cggtaccggc ggcaactgga tccggccctt cggccacgtg cacggcggca
    25861 tgagcctgcg cctgatcaag accgcgggcg agccgccggc ggtcacgctg caccgcctgc
    25921 cgctcgcgcg cttgcgcgag caggggctgg cggcgctcga tggcctcgcc cccatcgtga
    25981 gcggcgaggt ggacgactga cgtgggtccg cattcatgcg gacaccacta aatgtccgga
    26041 taaatccgga cccacggaat ccggacccaa gacaggagag agcatgagcg acaccatcca
    26101 caacacgctg cgcttcgatt accgcggcgc gcgcgtactg gtcaccggcg gcaccagcgg
    26161 catcggcgcg gcgattgccg ccgcctaccg cgaggcgggc gccgaggtga ccatcaccgg
    26221 cacgcgcgcg gcggccgcgg actacgacga ggatcttcgt ggctaccgct accttcagct
    26281 cgacgtcgag gatgccgcgc aggtcgccag tgtggccgcc gcgcagcagc agctcgatat
    26341 cctggtcaat aacgccggcc tcgcactgcc gagcctcggg ctcgacgagt acgagcccga
    26401 cgtgttcgcg cgcgcggtga acatgcacct ggtctcggcc taccgcatgg ccaagggctg
    26461 ccagcccgcg ctgtcggcga gcagcatgcc gggcggcgcc tcgatcatcg gcatcgcctc
    26521 gatgagctcg tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagctggat
    26641 caccggccag acgctgccga tcgacggggg attcacggtt tcgggttgag gtcgtggtcg
    26701 cgggcatggc ccgctcctac agggaacgcc ggcccaagct gtaggagccg gccatgcccg
    26761 tgaccaataa ccgctcgacc gtaggcgcgg gccatgcccg cgattcatgg ggcgcgcaga
    26821 tcgcgccccc gcccatcgct aatcgagtcg cgccagccgg cactcgctcc agcgctcgac
    26881 cagcggcccc atcagctgca tgttgcgccg cgccacgccc cacaggtggc gcagcacctc
    26941 gggctcgcgt ccgggcggta tccgctccag cgcgtgcttc atctcgaggc gtgcgctggc
    27001 caggtcgcgg tgccgcgtgc cgagcagcgc atcgatgcct gcgagctcga gcgcatcggc
    27061 ctgcgcgccg tgcaggtggc ggcgctcgag ataacgtgca atgcgcgccg cgccttccgc
    27121 ggtggccttg gcgaatgcat cctccacgtg cggcgcgatc ccgcgctcga gttgttttgc
    27181 cagcacctcg tagagctctc ccgcgggatt gcgccccggg atttccagaa tctcggtgtc
    27241 cggctccggc atcccgtgat acatgccgat ggcctcgcag cacaccaggt cgcacacggc
    27301 ctggtagccc aggttcagtg ccacggtgct cttccagtcc gggcgctgca ccagcgccgg
    27361 gatcgtgatc accgaacgca ccacctggtg cgcgaggtaa tagattgcct tctgccgatc
    27421 gacgctgatg ccggcacgcg ccgcgtagtg cctcacgtag ggtgtcaggt cgccggcgca
    27481 gctcggcgtg aagaaatccc gcagccacag gttgccgatg tcctccatcg gatcgccgta
    27541 gtgcgcccac tcccagtcga ccaccgcggt cacgcgatgg ttctcgaaca tgaagttgcc
    27601 gggccccgtg tcaccctgca gcagcaccgt gcgttccacc gtgcgcggca cgtgattcag
    27661 cagccagcgc cggccgaagg cgccgagcac cgcgctcgaa tccaccggca gtttcgcgaa
    27721 gcgcggatca tcgacggcgc gcagttccag cagtgaatgg tcctcgggcg tcgccggcat
    27781 ctcgaactgc ggcaggtcca tcgtccgcac atcgaggcga tgcatcgcgg ccatgatttc
    27841 catgaagtgc agcatcacgt cgtgctgctc cgcgcgcgag gcgttgttca ggtcggcgcg
    27901 cccgggttcg aaggactgta tcgccacgca gtgctcgggg ctccacgcgt gcagctgctg
    27961 cgtcgggatg tcgtcgtgcc cggccaggtg acggatcagc gccgattcgc gcgcgaggtt
    28021 gcgcgggtag ggccggcccg cggcaagctc gcggtcgatg cgcaggaaca ccggctgcac
    28081 cgatccgtcg tcgcgctcga tgtcggcacg ccacgcctcg cggcgcacca ccatgcgctc
    28141 gagtgctgcg actcgtccgc ccagttgctg ctctatccac gccacggcgg ccgaggggaa
    28201 tgctgcctgc tctgctgtca tgggtgttct tctccgatgt tgttgtgtcg cgggttcggc
    28261 ttcagcccgg ccctgtccgc atgaatgcgg acccacgcgg gcaacgtccg catgaatgcg
    28321 gaccacgcac ggcctggcgt gtggcgagca aagcgcatcg tccccgaagc cggcgtcctt
    28381 gccaagcccg caccgggcgc ccggggtcgt tggggcagtc atccggcctg acagcatggg
    28441 tgatgagcgc gtttttgttg tgctgcaggg gtttacgaat ttactaagca cgcttatagt
    28501 aatgctaaat taacgtaggc atgggttacg atgtctgcaa gctccggttc tgagcccagc
    28561 gtcagcaggc tggtcaccga gatttcgcgg ctgttctcgc gtgatttcag tgcacgcgtg
    28621 cgcgactacg acctgaccca tacccagtgg caggccctgg cggtgctgtc ccggcaggaa
    28681 ggcatcaacc aggccgagct ggccaatctg ctgcaggtcc agccgatttc gctgggacga
    28741 ttgatcgatc gcatggaggc agcgggctgg gtggagcgcc gccctgcgcc caacgaccgc
    28801 cgcgcgatcc agctctacct cacgtccagg gtcgagccga tcctggacaa gatgacagag
    28861 gccgcatcgc tcacccgaga agcggccctc gccgatttcg ctaccgacga acgcgagcag
    28921 ctctgccgga tgctgcagcg cgtgaaagcc aatcttgccg gggacgatcc gcttgccaca
    28981 gcggttgcgc cgccgcaggc cggaagacgg acgagcaaca gatgaacgct gaagccgcgc
    29041 gcaaacctga atccgccggg cagcgtgccg gcacgctgca atcggtcggg actcccgtga
    29101 gcgcagcgcc ggtagcggat ggcggggcgg gctctgccgc gacgactgcc ggcgtactcc
    29161 cgacggatgc gaccgcactc gaaccctaca gccctcgccc gcgactgcgt cgccgcctgc
    29221 tcgttgccgg accgctcatc gtggccctgg cggggctgct gctgtggttc agcaccgggc
    29281 gctacgccag taccgaggac gcctacgtga aggccgacaa ggtcacgatc agcgcgcagg
    29341 tggccggccc tatcgccggg gttgccgtgc gcgagaacca gcgcgtacgc gacggcgacg
    29401 agctgttccg tatcgacgac cgcccctacc gcattgccct tgccggcgcc gaggccgagc
    29461 tcgcggcggt cgccaccgag ctcgacggca tcaaggcgag cctgcggcag aagaacgagg
    29521 agctgcgcct ggcgcacacc aaccgcgatt tcgccgcgcg cgaattcgag cgccagtcgg
    29581 agctggcgcg caagaagctg aactctggcg cggccgtcga cgaggtcagg tacaagctcg
    29641 atgtcgcgct gcagcagatc agtgtcacgg agcaggcgcg cgcgcagcac ctgtcgcgcc
    29701 tgaacggcaa tcccgagctg ccgctggaac agtacccggc ctaccaggcg gccagggcga
    29761 gccgcgatga cgccgcgctg gatctggagc gcacggtggt gcgcgcgccc ttcgcgggca
    29821 tcgccagcaa gacgccgcag cgcggacagt acgtcgctgc aggcagcgcc gtgatgagcg
    29881 tcgtgggggc ggacgaggtg tggatcgagg cgaactacat ggagaccgat ctcacccacg
    29941 tgcgcgtcgg gcagccggtc tcggtgcaga tcgattccta ccccggacgc cattggcgcg
    30001 gcaccgtgca gagcatcagc caggcgacgg gggccgagtt ctcggtgctg ccgccgcaga
    30061 acgcctcggg caactgggtc aagatcacgc aacggatccc gttgcgcatc gccatcgaac
    30121 cccgcgccga tgatccgccg ctgcgcgcgg gcatgaccgc gaccgtggca atcgacaccg
    30181 gcgaccgtca cacgctgaag gcgttgctgg gcggcatcgg cggctcggtg gatgaccatc
    30241 gcgcggcgat cccggacggt agtacgcatt ccgctccatg agcgtggccg gcgacgcgga
    30301 gcacgtgccc acgaagcacc cgtggctgct gctgtgcacc atgatgctga cgacggtgat
    30361 ggtggcgctc gattcgacca ttgccaacgt ggtgctgccg cacatgcagg gttcgctgtc
    30421 ggcggcgcag gaccagatag cctgggtgct gacctcgtat atcgtcgcca ccgccatctt
    30481 catcccgccg accggttacc ttgccgcgtg cctcggtcgc cagcgcctgc tgggcatctg
    30541 cgtcggcggc tttctcgccg cctccatgct gtgcggcacg gcgacctcat tgcccgaaat
    30601 ggttgcctac cgcttcctgc agggagcgtt cggcgcgggc atggcaccgc tggggcaggc
    30661 cctcgtgatg gacagctttc cccgggaaaa acacgggcag gtactgtcgc tgtggggtgt
    30721 tggcgccatg ttgggcccga tcctcggtcc gaccctcggg ggctatctca ccgaagtcct
    30781 caactggcgc tgggtcttct acatcaacgt gccgatctgc gcggtggcgc tgctcggcat
    30841 ctggaagacg gtgccgcaag tcaaggcccg gcgcgacgtg cccttcgaca tgttcggctt
    30901 cgccctgctc agtctcgcgc tgggtgcggt tcagctgatg ctcgatcgcg gacactcgca
    30961 gaactggttc tcgtccatgg aaatcgtcat cgaggcgacc gtggcaggcc tttgcttcta
    31021 cctgttcctg gtgcacatgt tcaccgcccg cgcgccgttc atcgagccgc ggctgttcaa
    31081 ggaccgcaac ctcgtcgtgg ggctggtgct gattgccgtc gtcagcatgg tgatgatggc
    31141 gcagatggcg ctgctgccga gcttcctgca gcagctgatg cacatcccgg tcgacaccgt
    31201 cggcatgatg ctgatgccgc gcggactggc gtcgatgatc gggatggtgc tgacgtcgcg
    31261 cctgatcggt cgcgtcgacc agcgcgcgat gatgatttgc ggcatggtgg ggttcgggtt
    31321 gtcgacctat gagatgtcga ccatcaacct gtacgtggac aacttcacga tcgtgcgcat
    31381 cggtctcatg cagggcttca gcatggcctt cgtggtgtcg cccctgtcga tcgccgcgtt
    31441 ctccaccctg gcaccggacc tgcgcaccga gagcgcctcg atgttcgccc tgatgcgcaa
    31501 catcggcggc agcgtcggcg tttcggtgct gttcacccgg gtggcccagg tgacgcaggc
    31561 cgagcatgca cgtctcggcg agagcatcac cccgttctcc gatgcgctgc ccccggcgtg
    31621 gcagtgggac accatcgcag gggccatgag cctcgacggg gagatcgtcc ggcaggcggc
    31681 cagcatcgcc tacgtgcaca ccttcctgat gatggcggtg ctggccctgc t
//
LOCUS       DAORUQ010000012        31246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_994_length_31246_cov_6.488699, whole genome shotgun sequence.
ACCESSION   DAORUQ010000012 DAORUQ010000000
VERSION     DAORUQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 31246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31246
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_31246_cov_6.488699"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLW13_02225"
     CDS             complement(<1..538)
                     /locus_tag="PLW13_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69212.1"
                     /translation="MQLPEAVSNVLDAGGIPWSPAELAAGPAQAVVRPVMLQDAAGRV
                     LVLVAADSLLDLDALNRCTARELIATPAEALAPLCAARGLHSLPAVPGVLELPVIADQ
                     RLLASSELGLDAGAGGAQLQMSGAAFRALLESRGGATADFCVALDDIPPAAAASADVA
                     EIGNAVARFTARRIERRLE"
     gene            complement(650..2746)
                     /gene="recG"
                     /locus_tag="PLW13_02230"
     CDS             complement(650..2746)
                     /gene="recG"
                     /locus_tag="PLW13_02230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQY69213.1"
                     /translation="MTPDSASPRSSLEQLPVTALRGVGPRLAEKLAALEIRSVADLLF
                     HLPLRYQDRTRVSAVRELRPGAEAVVEGRVLAARVELGRRRSLACTLADASGTMTLRF
                     FHFSAAQQHNLERAARLRCYGEARLGRSGIEMYHPECQVLDADEPAPDAAALTPIYPS
                     TEGLHQASWRSLTEQALRHLGSNTLRELLPAGLLRGAGPLAEALRYLHRPPPDAPVAE
                     LLAGTHPYQQRLAAEELLAHHLGLLLMRKRARTHRAPVMHDDGRLGERLLRSLAFTPT
                     GAQLRVSAEIRRDLASGAPMLRLVQGDVGSGKTVVAALASLAVIENGWQVAIMAPTEL
                     LAEQHYRTFSAWLDPLGLRTVLLSGRMPAAQRRATLEALALGETPLAVGTHALFQEQV
                     GFARLGLVIIDEQHRFGVHQRLALREKGLADGFAPHQLVMTATPIPRTLAMTAYADLD
                     YSLIDELPPGRTPVATAVVSNARREEVVERVRAACAEGRQAYWVCTLIEESETLEAQA
                     AEAIAAELAERLPALRVGLVHGRVPGRQRLEIMNAFKAGEIELLVATTVIEVGVDVPR
                     ASLMIIENAERLGLAQLHQLRGRVGRGRDASHCVLLYQAPLSLAGRARLDAMRDTCDG
                     FVLAERDLELRGPGELLGTRQTGLMGLRFADLGRDGALLPAVRAAAETMLREHPGRID
                     ALLDRWIPGRTRYAEA"
     gene            2809..3669
                     /locus_tag="PLW13_02235"
     CDS             2809..3669
                     /locus_tag="PLW13_02235"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69214.1"
                     /translation="MNGADILIAGCGDVGGALAERLRDAGHRVAGLRRRAGLLPAGIE
                     PLAADLGEPATLALLGERRFDIVVVSSAAGRFDEDHYRRVYVEGLANLLDVLRGTPHV
                     LLASSTAVYHQCDGEWVDEDSPTEPHGFSGRILLQAEALLRERAAHRATVLRFGGIYG
                     PGRERLLREVAAGIGCAREPVRYTNRIHREDCAGILQFLVGRLLRGETLAPLYLGVDS
                     EPAPMWEVRHWIAAELGVALDDAAGGAQGGRAPGSKRCSNRRLLEAGYALRYPDFRAG
                     YAPLIAPLRT"
     gene            complement(3659..4048)
                     /locus_tag="PLW13_02240"
     CDS             complement(3659..4048)
                     /locus_tag="PLW13_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY69215.1"
                     /translation="MSNRAVIHASDAPQAIGPYSQAVKINNVVYMSGQIPLDPATMQL
                     VAGDITEQTVRVFENLAAVARAAGGDLNHCVKVNIYLTDLADFAAVNAVMARYFAEPY
                     PARACVQVAALPRGAAVEIEATMVVKS"
     gene            complement(4080..6197)
                     /gene="spoT"
                     /locus_tag="PLW13_02245"
     CDS             complement(4080..6197)
                     /gene="spoT"
                     /locus_tag="PLW13_02245"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016712710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HQY69216.1"
                     /translation="MQHLGALTEKLRGYLAPEQVNAVRRAYYYSEQAHDGQVRRTGQP
                     YVTHPLAVAGILADMRMDHQSLMAALLHDVIEDTGIAKEALASQFGTSVAELVDGVSK
                     LSQFETTSRAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLDVLKPEKRQRIAQ
                     ETLEIYAPIAQRLGMNNMRIEFEDLGFTALHPMRARRIQAAVRKARGNRKELVTQIQR
                     KIETRLREEHIPARVLGREKHLYSIYQKMRAKGRSFSEIMDVFAFRIVVDSVDSCYRV
                     LGAMHSLYKPVPGHFKDYIAIPKANGYQSLHTVLFGMHGVPIEIQIRTADMEEMANNG
                     IAAHWLYKSSGSEKPGSGHSRARQWVQGLLEMQQRAGNSLEFIESMKIDLFPDEVYVF
                     TPRGDIFALPNGATAVDFAYTVHTDIGNSCVACRIDRNLAPLSTRLQSGQTVEIVTAP
                     RANPNPTWLNFVVTAKARTNIRHYLKGQQQSESIELGRRLLDKALVSLQSSMKTIDPR
                     TVEQLLAETRRASFDTILEEIGLGNRVALLTARRLLERGEGIPGASAAEALPLAIRGT
                     EGMVINFGKCCYPIPGDTIVGHISAGRGVVVHRDSCNNIAEFLDDPERCTPLRWAEDT
                     RGEFSVELLVELENQKGVIAALANRISSLDVNIEKIGMEERDARLSQVRLVIAVANRV
                     NLARIIRRIRTVRSVMKVTRVKR"
     gene            complement(6334..6510)
                     /gene="rpoZ"
                     /locus_tag="PLW13_02250"
                     /pseudo
     CDS             complement(6334..6510)
                     /gene="rpoZ"
                     /locus_tag="PLW13_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            complement(6592..7221)
                     /gene="gmk"
                     /locus_tag="PLW13_02255"
     CDS             complement(6592..7221)
                     /gene="gmk"
                     /locus_tag="PLW13_02255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQY69217.1"
                     /translation="MNTTAPGTLYTVSAPSGAGKTSLVAALVGQDPDLLASVSHTTRA
                     RRPGEVEGVNYHFTSREQFEQMLGRGDFLEHATVFGNLYGTSRSWVMETLAGGKDVIL
                     EIDWQGARQVRERIRAAQSIYILPPSRDTLESRLQGRKQDGAEVIGERMRQAVSEMSH
                     WDESDYVVVNDRFEVALADLQAILRANRLRRPAQGMRLSGLIRELLSAT"
     gene            complement(7218..8072)
                     /locus_tag="PLW13_02260"
     CDS             complement(7218..8072)
                     /locus_tag="PLW13_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQY69218.1"
                     /translation="MTAFARREASFAWGTLSWEIRSVNHRYLEAQLRLPETLRQVEMP
                     ARELLRERLARGKVDCILRLQAGPSATGPIAINDDLVRSLHEACARVDALAGGLGRPG
                     VLDLLRWPGVIAESHPDEDEIARSALAVFRQALDELIAMRAREGASLASAIEQRLAGI
                     ETIVASVRAVLPGILAAQKQKLVDRLAELRVEVEPGRLEQEIALLAARSDVAEELDRL
                     QSHVAEARHSLAAGAACGRRLDFLMQEFNREANTLSSKSASAETTRSAVELKVLVEQM
                     REQVQNIE"
     gene            8209..8943
                     /gene="rph"
                     /locus_tag="PLW13_02265"
     CDS             8209..8943
                     /gene="rph"
                     /locus_tag="PLW13_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQY69219.1"
                     /translation="MERPSGRRPDQLRPVRFTRAYTRHAEGSVLVEYGDTKVICTASV
                     DKGVPRFMRGQGRGWLTAEYGMLPRSTNTRMDREAARGKQGGRTVEIQRLIGRSLRSA
                     LDLRKLGENTIVIDCDVIQADGGTRTASITGACVALCDALAAAKRRKLITEDPLLHLI
                     ASVSVGMYNGHAVLDLDYAEDSSAETDMNVIMTEQGDYVEVQGTAEAKPFKAEELQQM
                     LDLARRGTENLVRVQKMALSAPLPAK"
     gene            complement(8960..9736)
                     /locus_tag="PLW13_02270"
     CDS             complement(8960..9736)
                     /locus_tag="PLW13_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY69220.1"
                     /translation="MRVISFCADGIREAAAKGFYDWAVDQDADIICVQDLRAPESGLG
                     AAKYNPDGYFSYFFDAIDGTTNGVAIYCRKLPKAIMTGLGLGEADGEGRFMQADFENI
                     SVACLLGPVASMEDTGSLARKSRFYEQLRTQLDKVRKKRRQYVLCGNWNMAHAARDVQ
                     QAAVHADTPGFLAAERQYLDGLYGELGYVDAFRVVNQDEDEFSWWPGGRAAGDGWRVD
                     LQIVSAGLRGTIEYGALYKVQEFSSHAPLIMDYDIELERL"
     gene            9879..10535
                     /gene="pyrE"
                     /locus_tag="PLW13_02275"
     CDS             9879..10535
                     /gene="pyrE"
                     /locus_tag="PLW13_02275"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQY69221.1"
                     /translation="MSATGFRNEFIALALRQRALRFGSFTLKSGRTSPYFFNAGEFSS
                     GGALAALGRCYAAAIAAAGVEYDMLFGPAYKGIPLAVATAVAISNDSGRDVPCAFNRK
                     EAKTHGEGGTLIGAPLAGRVLIIDDVITAGTAVRGVVDLIAAAGATIAGVVLGLDRAE
                     RGSGADSAVTELARELGVPVFSIARLDDIVRFLEESEGFGEHLPQLLAYRRQWGEAGA
                     "
     gene            10599..11255
                     /locus_tag="PLW13_02280"
     CDS             10599..11255
                     /locus_tag="PLW13_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69222.1"
                     /translation="MTSRPFHRDVPALPRWLGVVALLYLLGAPASALAEDRFYKYDNA
                     EGIPVIDDRVPPELAHKGYTVLNRAGRVVEVVPRTLTAAERKANPGAVQAMLRAEEAE
                     RQRRYDEALLARYSSVADIEDLQRRKVNEVQVRINLQKGSIAALKQQLESEHAKAADL
                     ELAEAPVPADLQKKIEELRASIAEEEARIARLEQDRSTVQARYQFDIERFRAIRPGSR
                     "
     gene            complement(11263..11859)
                     /gene="slmA"
                     /locus_tag="PLW13_02285"
     CDS             complement(11263..11859)
                     /gene="slmA"
                     /locus_tag="PLW13_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HQY69223.1"
                     /translation="MNNKTSRRDAILQSLAHMLEATPGGRITTAALAADVGVSEAALY
                     RHFPSKAKMFEGLIEFIETTLFSRISRIREEEQDAAAQCEQIVTLLLTFCERNPGITR
                     LLTGDALTGETDRLRGRVQQLFDRLETELKQILREAEVRQGLRTALPVNLAANLLLAT
                     AEGRIIQYVRSEFRRSPTDYWAEQWRVLTGSLFRRAGD"
     gene            complement(11911..12831)
                     /gene="argB"
                     /locus_tag="PLW13_02290"
     CDS             complement(11911..12831)
                     /gene="argB"
                     /locus_tag="PLW13_02290"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQY69224.1"
                     /translation="MSLNREAAVNIARVLTESLPYIQRFIGKTIVIKYGGNAMVDPQL
                     KESFARDVVLMKLVGMNPVVVHGGGPQIGSLLAKLNIESRFVEGMRVTDTQTMDVVEM
                     VLGATVNKEIVSNINRHGGNAIGVTGKDGHLIEASRMSVTVRTAETSAPEIVDIGHVG
                     EVRRINRAVLDMFLHSDFIPVVAPIGVDKAGLSYNINADLVAGKIAEVLQAEKLMLLT
                     NVPGLMDAEGKVLTGLDRDEVNRLIDEGVIRGGMLPKIRCALDAVASGVTSAHIIDGT
                     VPHAVLLEVFTDEGVGTLICNRKPPVVAGA"
     gene            complement(12914..15340)
                     /locus_tag="PLW13_02295"
     CDS             complement(12914..15340)
                     /locus_tag="PLW13_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQY69225.1"
                     /translation="MTSAVPSTGLAPARRLVVRAIGIVSVLLLAAAVAYSWTTELLAH
                     RERAQATARQAALSISTYGHLLAAHAQALAHDATGAPERAPRSAFPGVQSVQFIPLGP
                     LGIADPAFRKEVLRNNIEIRLAGRAFNGENPATEAYRDNGGWILVAAAAVPGSADHAG
                     GVVLARADLATVVRDHLPLAHESGEFSVWSQGTDGNYLPMVRTGRADGYTEPSYRAAT
                     GIRPLYAAFEPASRVPLRLSLGITALALGAGAAALLLIALVRRTFRALAAGLHDDAAK
                     LTDLALRQRNTTATLPALSWHELAGLGEALVQLRRDQQPGTAEPAPLAARPARPAPDP
                     LEIVEQLAETGEEEAPPDIPAEIFRDYDIRGHSAQITSQLAHAIGQAIGSEALDRQCT
                     SIMVGADGRDSSPRLREHLVNGLLSTGIDVIDTGTVATPMLYFACHHLRTMSGVMITG
                     SHNPAEHNGFKIMIGGETQRGAGILRLRERIEHALFHEGQGSYRVTEIDADYIKAVAD
                     DVLVDGRHKVVIDCGNGAASVIAEDLFRELGCDVVPLFCELDSRFPNHHPDPAVPENL
                     QALIAKVAETGAALGIAFDGDADRIGVVSASGTIVTADRLMMLLVRDVLASHPGADVV
                     FDVKCSRDLAAVVRENGGRPIMCRSGHSWIKEKMKESGALLGGEFTGHICFRDRWFGF
                     DDALYCAARLLELLGAGSSTLDELLAQLPHSVATPEIRIEMPEEQKFAAMEAISRGFS
                     LELENARLSRIDGVRAEFADGWGLVRASNTSAALICRFEGRDEATLARIQAAFLAEIR
                     RLLPGTNVSF"
     gene            complement(15337..16560)
                     /gene="coaBC"
                     /locus_tag="PLW13_02300"
     CDS             complement(15337..16560)
                     /gene="coaBC"
                     /locus_tag="PLW13_02300"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQY69226.1"
                     /translation="MATLNNRQILLGVTGGIAAYKSAELVRRLRDAGAVVRVVMTRAA
                     CEFITPLTMQALSGNPVHRELLDETAEAAMGHIELARWADLVVVAPASADFIARLAHG
                     LADDLLATLCLATDAPVLVAPAMNQAMWRNPATRHNCGVLTQRGVDLLGPGSGSQACG
                     DTGPGRMLEPAELLAALASRFAPRDLDGVRVMITAGPTREALDPVRYISNHSSGKMGF
                     AIAAAAAEAGAGVTLVSGPVHLDTPARVARIDVSSAMEMHEAVMSRAAQCDIFIACAA
                     VADYRPEHCAGQKIKKSTERMEVRLVRNPDIVADVAALESRPFTVGFAAETTDVVAYA
                     RGKLAAKRLDLIVANDVARGDIGFNSEFNEVCLISATDTTELARGTKAQIARALVAAI
                     GARFREGNPAAGEAS"
     gene            16676..17350
                     /gene="radC"
                     /locus_tag="PLW13_02305"
     CDS             16676..17350
                     /gene="radC"
                     /locus_tag="PLW13_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQY69227.1"
                     /translation="MTIKSWPAAERPRERLLGRGASTLSDAELLAILLRTGLPGGTAV
                     DRARELLGAFGDLRQLLDAEPAAFCATHGLGEATYAHLQAALELSRRYLLSRISRGQA
                     LGSPQEVRQFLQLKMRCLPHEVFACLFLDNQHTVISYEELFRGTIDGASVYPREIVKR
                     SLALNAAAVIFAHNHPSGVAEPSQADQRLTQRLKSALGTVDIRVLDHFVVGESEALSF
                     AERGLL"
     gene            17480..17716
                     /gene="rpmB"
                     /locus_tag="PLW13_02310"
     CDS             17480..17716
                     /gene="rpmB"
                     /locus_tag="PLW13_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQY69228.1"
                     /translation="MSKVCQVTGKRPVVGNNVSHANNKTKRRFEPNLHKHRFWVEAEK
                     RFVTLRVSAKAMRIIDKLGIDSVIADMRARGERV"
     gene            17740..17895
                     /gene="rpmG"
                     /locus_tag="PLW13_02315"
     CDS             17740..17895
                     /gene="rpmG"
                     /locus_tag="PLW13_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQY69229.1"
                     /translation="MRDKIKLVSSAGTGHFYTTDKNKRTTPDKLEFKKYDPVVRKHVA
                     YKEAKIK"
     gene            17991..18809
                     /gene="mutM"
                     /locus_tag="PLW13_02320"
     CDS             17991..18809
                     /gene="mutM"
                     /locus_tag="PLW13_02320"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HQY69230.1"
                     /translation="MPELPEVETTRRGILPCLSGRRIRALEVREPRLRWPVPSDLDAI
                     LRGEEIDTVERRGKYLLVRLGKGTLLIHLGMSGSLRVLTAPAAAPRKHDHIDLLLEDG
                     QLLRYHDPRRFGAFLWLEGDPAAHPLLAGLGPEPLGAGFDGDYLFRLSRGRRTSVKQF
                     LMDGHVVVGVGNIYANEALYLAGIHPRRQAGRIAAARYARLADAVRRVLEGAIAQGGT
                     TLRDYVNGNGVPGYFALSLSVYGRTGRPCPGCGEAIRQARNAQRSTFYCPRCQR"
     gene            18954..19220
                     /locus_tag="PLW13_02325"
     CDS             18954..19220
                     /locus_tag="PLW13_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69231.1"
                     /translation="MTPWFANAQPVDENPSALAMIGDLVIARPLLVVATAAGAAAFLV
                     SLPFTATGGNMDQAADTLMAGPAQAAFVRCLGCRSSGRRQAVSD"
     gene            complement(19228..19479)
                     /locus_tag="PLW13_02330"
     CDS             complement(19228..19479)
                     /locus_tag="PLW13_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HQY69232.1"
                     /translation="MALLITTQCINCDVCEPVCPNEAIYPGEEIYEIDPARCTECVGH
                     FDEPQCRKVCPVDCIPLDPAHPESRQELLLKFERLCGSH"
     gene            complement(19492..19977)
                     /gene="coaD"
                     /locus_tag="PLW13_02335"
     CDS             complement(19492..19977)
                     /gene="coaD"
                     /locus_tag="PLW13_02335"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQY69233.1"
                     /translation="MRTIVYPGTFDPITIGHVDLVERACRLFDHVVVAIASSERKRPL
                     FSLDERIGLCQAALGHLQNVEIVGFDILVVKFLRERKGCAVLRGLRAVSDFEYEFQLA
                     NMNRAMDPDFETVFLTPADHLSFISSTLVREIASLQGDVTKFVHPRVRAALLEKFADP
                     A"
     gene            complement(20081..20677)
                     /gene="rsmD"
                     /locus_tag="PLW13_02340"
     CDS             complement(20081..20677)
                     /gene="rsmD"
                     /locus_tag="PLW13_02340"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQY69234.1"
                     /translation="MAARKPAPAAGHASRSQVRIIGGSWRGRRIEFQAEPGLRPTPDR
                     VRETLFNWLQPQLPGSRCLDLFCGSGALAIEALSRGAAGATLVDLSARVLREVRHNLE
                     RLGTSAAELVAADAVAWIETVRDRRYDIVFIDPPFGSGTAQRCLRLLAGSALLAPGAR
                     VYLECGVAEAAPELPPGWLLHREKCAGQVAYRLYLAPG"
     gene            20761..21876
                     /gene="ftsY"
                     /locus_tag="PLW13_02345"
     CDS             20761..21876
                     /gene="ftsY"
                     /locus_tag="PLW13_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQY69235.1"
                     /translation="MLRFFSRKKPDAAAPDAAAAPVVVPVPVPEPEPETETEPPAAAP
                     MLQASHDAPPAQTGDAARPGWFSRLRAGLGKTRNAFSEGLGTLFLGRKTLDEDLLEEL
                     ETRLLLADVGMPTVRLIIDGMVQRIKRKELDDPQALYRALQEQLVAVLAPASVPLAID
                     TARKPFVILMVGVNGAGKTTTIGKLARRYQAEGRSVMLAAGDTFRAAAVEQLQIWGER
                     HGVPVVAQPTGSDSASVIFDALQSAQARGCDVLIADTAGRLHVKGNLMEELRKIVRVM
                     QKVDPGAPHEVMLVLDAGTGQNGLAQARHFREAVGVSGIALTKLDGTAKGGIVFAVAR
                     ELGLPIRYIGVGESAEDLREFEAQEFVAALFDDSARP"
     gene            21963..>22750
                     /gene="rpoH"
                     /locus_tag="PLW13_02350"
     CDS             21963..>22750
                     /gene="rpoH"
                     /locus_tag="PLW13_02350"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HQY69236.1"
                     /translation="MSKELQPLMALTPGGNINAYIQAVNAFPVLTPEREQALGEALYF
                     RDDLAAARELVLAHLRFVVHIARSYSGYGLPLADLVQEGNVGLMKAVRRFDPQKGVRL
                     VSFAVHWIKAEMHEYILRNWRIVKIATTKAQRKLFFNLRSVKKSLGWLTNDEAAAMAR
                     DLGVGIDEVRRMEGRLASADVAFDAGTDDDDDAAQFAPVHYLEDRRFDPARELEDSDW
                     EASSQERLEHALEGLDERSRHIVQSRWLNEDKATLHELAARYGVS"
     assembly_gap    22751..22850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22851..23129
                     /locus_tag="PLW13_02355"
     CDS             <22851..23129
                     /locus_tag="PLW13_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HQY69237.1"
                     /translation="AALAVWATAADYQMYHSLALLALGLARPAALAPRWRRVAVACFA
                     VGIVLFSGSLYLLALSGIRALGMITPLGGVLFLLGWVAVAAGAWRSEH"
     gene            23119..23850
                     /gene="trmB"
                     /locus_tag="PLW13_02360"
     CDS             23119..23850
                     /gene="trmB"
                     /locus_tag="PLW13_02360"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQY69238.1"
                     /translation="MSTDEQELRERPIRSFVRRQGRFTPAQRDAFDSLWAGRGLEPGD
                     TLLDLPLLFPGCTAFVVEIGFGMGHTLVELASRSPQTGFVGIEVHRPGVGKLLAMAEE
                     RGLQNIRVFCHDAVEVLNRAIPDASLDGLLLFFPDPWHKKRHHKRRLVQPPFVALVQR
                     KLKPGGLFHLATDWEDYARQMLEVVESVPGWLNLAGAGCFSPRPESRPLTRFEQRGMR
                     LGHGVWDLLLRKGEDQANSASTAFR"
     gene            complement(23817..24956)
                     /gene="hemW"
                     /locus_tag="PLW13_02365"
     CDS             complement(23817..24956)
                     /gene="hemW"
                     /locus_tag="PLW13_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQY69239.1"
                     /translation="MLTLPPLALYIHLPWCERKCPYCDFNSYQREAQPPFAAYAARLL
                     EDLGQDRGFAAGREAGSIFIGGGTPSLCPAEVVDLLLAGVRERLALAPDAEVTLEANP
                     GSAEAARFRGYRAAGVNRLSIGVQSFADGCLRALGRVHDARQAHAAIAAARDAGFASF
                     NIDLMHGLPHQSPAMALADLEQAIAHEAPHLSWYQLTVEPNTAFHSAPPPLPAEDELA
                     EIQDRGEELLARHGYEQYEVSAWARPGHQCRHNLNYWHFGDYIGIGAGAHGKLTLPAQ
                     GRVLRTRKTRVPKDYLAGAATSIRTDTAVAPDELALEFLMNALRLREGASVAQFEART
                     GLAAATIGAQLGALRAHGLMETDPARLCTTARGFRYLNAVLAEFA"
     gene            complement(24950..25552)
                     /gene="rdgB"
                     /locus_tag="PLW13_02370"
     CDS             complement(24950..25552)
                     /gene="rdgB"
                     /locus_tag="PLW13_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQY69240.1"
                     /translation="MRLSELVIATGNRGKLAELHALLADSGIEVRSQEDFAVPGIAET
                     GLSFVENAIIKARHASAHTGLPALADDSGLEVDALAGQPGVHSARYAGEPCDDAANNR
                     KLLEALADVPAARRTARFHCVLALLRHPADPVPLICAGRWEGRILQAPAGENGFGYDP
                     LFLVETEGCSSAQLPRELKNRLSHRAQALGQLLLALGKPC"
     gene            complement(25563..26021)
                     /locus_tag="PLW13_02375"
     CDS             complement(25563..26021)
                     /locus_tag="PLW13_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HQY69241.1"
                     /translation="MKHSSRRSPVATLAVSLLLALLALLATPTRAEMRQYGSFEVHYS
                     VFASSFLQPDIAAAYGIVRAKDRALLNIAVRRGTRDAATVSAVLSGTRGDLIRKTPLE
                     FREIREDQAVYYIAEFGFVSGETQYFDIAIQPAGEARPLVLQFNHVLYAD"
     gene            complement(26018..26608)
                     /gene="metW"
                     /locus_tag="PLW13_02380"
     CDS             complement(26018..26608)
                     /gene="metW"
                     /locus_tag="PLW13_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HQY69242.1"
                     /translation="MRVDFDIIQQWVKPGARVLDLGCGDGKLLADLTQDRQVRGYGLE
                     IDPIKISLCLERGVNVIEQDLDRGLGNFRSDSFDTVVMSQTLQAVRYPHIILDEMLRI
                     GRECIITFPNFGNWRCRLQIAGKGRMPVSRFMPYTWYDTPNIHFCTVLDFEALCREKN
                     IHVINRVVVDANQRATPAARLLPNLCGVTAIYHVTK"
     gene            complement(26598..27773)
                     /locus_tag="PLW13_02385"
     CDS             complement(26598..27773)
                     /locus_tag="PLW13_02385"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQY69243.1"
                     /translation="MEPRAAAGSVGIVTPRTQRFEQPLELACGRTLPGYDIVYETYGT
                     LNADRSNAVLICHALSGNAHAAGYHSPADRKPGWWDNCIGPGKPIDTSRFFVVSLNNI
                     GGCAGSTGPHSVNPATGRIWGPDFPVVRVRDWVATQRRLADLLHIERWAAVVGGSLGG
                     MQAMRWTIDHAERVRHCVVIAAALKLSAQNLAFNEIARKAIMADPQFHEGWFLEHGTL
                     PRQGLAIARMIGHVTYLSDELMGDKFGRELRSGSFAPGSEADAEFQVQSYLRYQGDQF
                     ADNFDANTYLLMTRALDYFDLARDFGDDPVEAFRRARARFLVVSFSSDWRFSPRRSRE
                     IVDALLAAHRDVSYVEIEANEGHDAFLLPIPRYHDALQRYMEQVHAGLAPRSVADAR"
     gene            complement(27826..28134)
                     /locus_tag="PLW13_02390"
     CDS             complement(27826..28134)
                     /locus_tag="PLW13_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HQY69244.1"
                     /translation="MAETGPFWRWQGEVLSLRCQVQPRASADRIIGEHGGRLRIRISA
                     VPSEGAANERLCRFLASEFGVAPGAVEIGTGHGSRFKTALVRAPARIPPDLGIAPARA
                     "
     gene            complement(28134..28721)
                     /locus_tag="PLW13_02395"
     CDS             complement(28134..28721)
                     /locus_tag="PLW13_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQY69245.1"
                     /translation="MGTFAEISRYLVQTAFGVLLFAVLLRLLLQLARADFYNPISQFI
                     VKVTSPLLRPLRRIIPPLGRLDSASLVLALAIQCIATLVVLALLGFSIGNPLLLLAWS
                     LLGILSLLLNIYFFGILLLIVFSWIAPQSHHPGIALLYQLTEPVMAPVRRLLPPLGGL
                     DLSPILVFVIINVLEILLRGLAQGVGLPRGVVLGF"
     gene            complement(28741..29565)
                     /gene="proC"
                     /locus_tag="PLW13_02400"
     CDS             complement(28741..29565)
                     /gene="proC"
                     /locus_tag="PLW13_02400"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQY69246.1"
                     /translation="MTRSNIAFIGGGNMSGSMVGGIIARGWDPRAIRVSDPDAGCRER
                     LGALGVLTSADNHEAAASADIVVLAVKPQQLRSACRDIAAAVAERGSLVISIAAGIGT
                     ASLQSWLGADVAIVRCMPNTPALLQASATALYATPAVTAEQRAAAHEILSSVGFVAWV
                     EDEDAMHAVTAVSGSGPAYFFLVMEAMQLAAENLGLDPALARQLTAQTALGAARMALE
                     TDVDVVELRRRVTSPGGTTERALRVLEERGLRRVFESALEAASNRSRELADELGQP"
     gene            complement(29600..30286)
                     /locus_tag="PLW13_02405"
     CDS             complement(29600..30286)
                     /locus_tag="PLW13_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQY69247.1"
                     /translation="MDTIAERTRALLGQIARLEARHGRAPGSVALVGISKTHGPERVR
                     EAHRAGLAQFGESYVQEALPKIEALADLPLTWHFVGRIQGNKTAAIAASFAWVHGIDR
                     LRIAQRLDEQRPAHLPPLDCCIEVNLCGEASKGGAAPHELAALARAIAALPRLRLRGL
                     MTLPAPSDGIDAQRLPFARLARCLAELQRDIPGLDTLSMGMSDDMEAAIAEGATLVRI
                     GTALFGPRGT"
     gene            30430..>31246
                     /locus_tag="PLW13_02410"
     CDS             30430..>31246
                     /locus_tag="PLW13_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQY69248.1"
                     /translation="MDITELLAFAAKQRASDLHLSAGLPPMIRVDGDVRRINLPPMDH
                     RQVHDLIYEIMNDKQRRDYEEFLETDFSFEVPGVARFRVNAFNQNRGSGAVFRTIPSK
                     VLTMEDLGMGQVFREISMEPRGLVVVTGPTGSGKSTTLAAMIDYINDNKYHHILTIED
                     PIEFVHESKKCLVNQREVHRDTHGFAEALRSALRQDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRIVDVFPAQEKSMVRSMLSESLMGVISQTLLKKV"
BASE COUNT         4727 a        10511 c        10868 g         5040 t
ORIGIN      
        1 cctcgaggcg gcgctcgatg cgccgcgcgg tgaagcgcgc gaccgcgttg ccgatttccg
       61 cgacgtcggc gctcgcggcg gccgcgggcg ggatgtcatc gagcgcgaca cagaaatccg
      121 ctgtcgcgcc gccacgcgac tccagcaggg cgcggaacgc cgccccggac atctgcagct
      181 gtgccccgcc ggcaccggcg tcgagaccga gttcgctgct cgcgagcagg cgctggtcgg
      241 caatcacggg caactcgagc acgccgggca ccgccggcag ggagtgcagg ccccgcgcgg
      301 cacacagcgg cgccagggcc tccgcgggcg tggcgatcag ctcgcgcgcg gtgcagcggt
      361 tcagtgcgtc caggtccagc aacgagtccg ccgcgaccag cacgagcacg cggccggcgg
      421 catcctgcag catcacgggc cgtaccactg cctgcgccgg gcccgcggca agctccgccg
      481 gtgaccacgg aataccgccg gcatccagca cgttgctcac ggcttcgggt aattgcatcg
      541 tcatggtcct ccgcgccggg gcggatgccc tgctgtcacg ctgagggaat tgtagtagag
      601 cgtgccgggg cccacacgcg cgccagccga ttcggtgacg cagtcggcat caggcctcgg
      661 cgtagcgggt gcgccccggg atccagcggt cgagcagcgc gtcgatacgc ccgggatgtt
      721 cgcgcagcat cgtttcggcc gccgcgcgca cggccggcag cagcgcgccg tcgcggccga
      781 gatcggcgaa gcgcaggccc atcaggccgg tctggcgcgt gcccagcagt tcgccggggc
      841 cgcgcagctc gaggtcgcgc tcggccagca cgaagccgtc gcaggtgtcg cgcatcgcat
      901 cgagccgcgc gcgcccggcc agcgacagcg gtgcctggta caacagcacg cagtggctgg
      961 catcgcggcc gcgaccgacc cgtccacgca gctggtgcag ctgcgccagc ccgagccgct
     1021 cggcgttctc gatgatcatc agcgaggcgc gcggcacgtc cacgccgacc tcgatcacgg
     1081 tggtggccac cagcagttcg atctcgccgg ccttgaaggc gttcatgatc tcgaggcgct
     1141 gtctgccggg gacgcggccg tggacgagtc ccacccgcag cgccggtagc cgctcggcga
     1201 gctccgcggc gatcgcctcg gcggcctgtg cctccagcgt ctcggattcc tcgatcaggg
     1261 tgcagaccca gtaagcctgc cgcccttcgg cgcaggcggc gcgcacgcgc tcgaccacct
     1321 cctcgcggcg cgcattgctg accaccgcgg tggcgaccgg cgtgcgcccc ggcggcagtt
     1381 cgtcgatcag cgaatagtcg aggtccgcgt aggcggtcat tgccagcgtg cgcggtatcg
     1441 gggtcgccgt catcaccagc tggtgcggcg caaaaccgtc cgccagccct ttctcgcgca
     1501 gcgccaggcg ctggtgcacg ccgaagcggt gctgctcgtc gatgatcacc agccccagcc
     1561 gcgcgaaacc gacctgttcc tggaacagcg cgtgcgtgcc gacggccagc ggtgtctcgc
     1621 ccagcgccag cgcctccagc gtggcgcggc gttgcgccgc cggcatgcgc ccgctcagca
     1681 gcaccgtgcg caaacccagc ggatccagcc acgccgagaa cgtgcggtag tgctgttcgg
     1741 ccagcagctc cgtgggtgcc atgatcgcga cctgccagcc gttctcgatc accgcgaggc
     1801 tcgcgagcgc ggccaccacc gtcttgccgc tgccgacgtc gccctgcacc aggcgcagca
     1861 tcggcgctcc gctcgcgagg tcgcggcgga tctcggcgct gacgcgcagc tgcgcgccgg
     1921 tgggggtgaa ggcgagcgag cgcagcaggc gctcgccgag tcggccgtcg tcatgcatca
     1981 cgggggcgcg gtgcgtgcgg gcgcgcttgc gcatcagcag cagcccgagg tggtgcgcca
     2041 gcagttcctc ggcggcgagg cgttgctggt agggatgggt gccggccagc agctcggcga
     2101 ctggcgcatc gggcggcggc cggtgcaggt agcgcagcgc ctcggcgagc ggtccggcgc
     2161 cacgcagcag cccggccggc agcagctcgc gtagcgtgtt gctgccgaga tgccgcaaag
     2221 cttgctcggt aagtgagcgc caactggctt gatgcaggcc ctcggtgctg gggtagattg
     2281 gggtcagtgc cgccgcgtcc ggcgccggct cgtcggcatc cagcacctgg cattccgggt
     2341 ggtacatctc gatgccgctg cgcccgagac gcgcctcgcc gtaacagcgc aggcgcgccg
     2401 cgcgctcgag gttgtgctgc tgggccgcgc tgaagtggaa gaagcgcagc gtcatcgtcc
     2461 cgctggcgtc ggcaagcgtg cacgccaggc tgcgccggcg gccgagctcc acgcgcgccg
     2521 cgagcacgcg cccttcgacc acggcctccg cgccggggcg caattcgcgc accgcgctca
     2581 cgcgggtgcg gtcctggtag cgcagcggca ggtggaacag caggtcggcg acgctgcgga
     2641 tctcgagcgc ggcgagcttt tcggccagcc gcggcccgac gccgcgcagc gccgtgacgg
     2701 gcagttgttc gagcgaagat cgcgggctgg cggaatcggg ggtcaagcgg ctggccgatg
     2761 acgtgggcgg actgcgatga tagcccaatg cagttgcggg aggcgtcgat gaacggggcg
     2821 gacatattga tagccggttg tggcgacgtg ggtggcgcgc tggccgagcg gttgcgtgat
     2881 gcgggccatc gcgtggccgg cctgcggcgt cgcgccgggt tgttgcccgc cggtatcgag
     2941 ccgctggcgg cggacctggg cgagccggcc acgctcgccc tcctcggcga gcgtcgcttc
     3001 gacatcgtcg tggtcagcag tgccgcgggc cgcttcgacg aggaccacta tcgtcgcgtc
     3061 tacgtcgagg ggctcgcgaa cctgctcgac gtgctgcgtg gcacgccgca cgtgctgctg
     3121 gcatcgagca ccgcggtcta ccaccagtgc gacggcgaat gggtcgatga ggattcgccc
     3181 accgagccgc acggcttttc cggccggatc ctgttgcagg ccgaggcgct gctgcgcgag
     3241 cgcgccgcac atcgcgccac ggtgctgcgc ttcggcggca tctacggccc gggccgcgag
     3301 cggctgctgc gcgaggtcgc cgccggcatc ggctgtgcgc gcgagccggt gcgctacacc
     3361 aaccggattc atcgcgagga ctgcgcgggc atcctgcagt tcctggtcgg gcgcctgctg
     3421 cgcggcgaga cgctcgcacc gctctacctc ggcgtggaca gcgagccggc gccgatgtgg
     3481 gaggtgcgac actggatcgc cgccgaactc ggtgtggcgc tggacgacgc ggcgggtggc
     3541 gcgcagggcg ggcgcgcacc cggcagcaag cgctgcagca accggcgcct gctcgaggcc
     3601 ggttacgcgt tgcgctatcc cgattttcgc gccggctatg cgccgttgat cgcgccgctc
     3661 aggacttgac caccatcgtg gcctcgatct ccaccgcggc gccacgcggc agcgcggcga
     3721 cctgcacgca ggcacgcgcc gggtaaggct cggcgaaata gcgggccatc accgcgttga
     3781 ccgcggcgaa gtccgcgagg tcggtgaggt agatgttcac cttcacgcag tggttgaggt
     3841 cgccgccggc ggcgcgcgcc acggccgcca ggttctcgaa cacgcgcacc gtctgctccg
     3901 tgatgtcgcc cgcgaccagc tgcatggtcg ccgggtcgag cgggatctgg cccgacatgt
     3961 agaccacgtt gttgatcttc acggcctgcg aataagggcc gattgcctgc ggcgcgtcgc
     4021 tcgcgtggat gactgcgcga ttgctcatgg gtgctcctcg atgcgaatgc ggggggacat
     4081 cagcgcttta cccgcgtgac tttcatgacg gacctgaccg tgcggatgcg gcggatgatg
     4141 cgcgccaggt tcacgcggtt ggcgaccgcg ataaccagcc gcacctggct gagccgcgcg
     4201 tcgcgctcct ccatgccgat cttctcgatg tttacgtcca ggctgcttat gcggttcgcc
     4261 agcgcggcga tcacgccttt ctggttctcc agttccacca gcagctcgac gctgaattcg
     4321 ccgcgcgtgt cctcggccca gcgcagcggc gtgcagcgct cggggtcgtc gaggaactcg
     4381 gcgatgttgt tgcagctgtc gcggtgtacc accacgccgc gcccggcgct gatgtggccg
     4441 acgatcgtgt cgccgggtat cgggtagcaa cacttgccga agttgatcac catgccctcg
     4501 gtgccgcgga tcgccagcgg cagcgcctcc gcggcgctcg ccccgggaat gccctcgccc
     4561 ctctcgagca ggcggcgcgc ggtgagcagc gcgacgcggt tgccgaggcc gatctcctcg
     4621 aggatggtgt cgaaggaggc gcggcgcgtc tcggcgagca actgctccac ggtccgggga
     4681 tcgatcgtct tcatgctgct ctgcaggctc accagcgcct tgtcgagcag gcgccggccg
     4741 agctcgatcg attccgactg ctgctggccc ttgaggtaat ggcggatgtt ggtgcgggcc
     4801 ttcgccgtca ccacgaaatt gagccaggtc ggattcgggt tcgcgcgcgg ggccgtcacg
     4861 atctccacgg tctgcccgct ctgcaggcgc gtgctgagcg gagcgaggtt gcggtcgatg
     4921 cggcaggcga cgcagctgtt gccgatgtcg gtatgcaccg tgtaggcgaa atccaccgcg
     4981 gtcgcgccgt tgggcagcgc gaagatgtcg ccgcgcgggg tgaagacgta gacctcgtcc
     5041 gggaacaggt cgatcttcat gctctcgatg aattcgagcg agttgccggc gcgctgctgc
     5101 atctcgagca ggccctgcac ccattggcgc gcgcgcgagt ggccgctgcc gggcttctcc
     5161 gatccgctgc tcttgtacag ccagtgcgcg gcgatgccgt tgttggccat ctcctccatg
     5221 tccgcggtgc ggatctggat ctcgatcggc acgccgtgca tgccgaacag caccgtgtgc
     5281 agggactggt agccgttggc cttgggtatc gcgatgtagt ccttgaagtg gccgggaacc
     5341 ggcttgtaca ggctgtgcat ggcgcccagc acccgatagc agctgtcgac gctgtcgacc
     5401 acgatgcgaa acgcgaacac atccatgatt tccgagaacg agcggccctt ggcgcgcatc
     5461 ttctggtaaa tgctgtagag gtgcttctcg cgcccgagca cgcgcgcggg gatatgttcc
     5521 tcgcgcaggc gggtctcaat cttgcgctgg atctgcgtca ccagttcctt gcggttgccg
     5581 cgggccttgc gcacggcggc ctggatgcgc cgcgcgcgca tcgggtgcag tgccgtgaag
     5641 ccgaggtcct cgaactcgat gcgcatgttg ttcatgccga ggcgctgggc gatcggcgcg
     5701 tagatctcca gggtctcctg ggcgatgcgc tggcgcttct cgggcttcag cacgtcgagc
     5761 gtgcgcatgt tgtgcaggcg gtcggcgagt ttgaccagga tcacgcggat gtcgcgcgcc
     5821 atggccattg ccatcttctg gaaattctcg gcctgcgcct cggcacgcga ggtggtctcg
     5881 aactgcgaca gcttgctgac gccatcgacg agctcggcga cgctggtgcc gaactgtgag
     5941 gccagcgcct ccttggcgat gccggtatcc tcgatcacgt cgtgcagcag cgccgccatc
     6001 aggctctggt ggtccatgcg catgtcggcc aggatgccgg ccacggcgag cggatgggtc
     6061 acgtagggct ggccggtgcg ccgcacctgg ccgtcgtggg cctgctccga atagtagtag
     6121 gcgcgacgca ccgcattgac ctgctccggc gccaggtaac cccggagctt ttccgtcagt
     6181 gcgccgaggt gctgcatgcg tggcggttgc gcaatcgcgc ggtgcgggag gtgtgctccc
     6241 gcaccgtcag tctcacaggt tggctgcgtc ggcctgcgct tcgagcagtt cctcgaagga
     6301 cggttcggcg ttgtcgtcga agaaaccggg gccgatcagg ccttcctcga tctcgcgcag
     6361 cgcgatcacc gtcggcttgt cggactcctc gtgcaccagg ggctccttcc cgccggtggc
     6421 gatctggcgc gcgcgcttgg ccgcgaccat gaccagctcg aagcggttct ccaccttgtc
     6481 gagacattcc tctaccgtaa ttcgtgccat ggatatcacc tgttgcgtgg ctttgagctg
     6541 cgacccgccg gcctccgggg tcgcgcgggg cacgcattct agcagagttg ctcaggtcgc
     6601 cgacagcagc tcgcggatca gccccgacag ccgcatgccc tgggccggcc ggcgcaggcg
     6661 attggcgcgc agtatggcct gcaggtcggc cagcgcgacc tcgaagcggt cgttcaccac
     6721 gacgtagtcc gactcgtccc agtgcgacat ctcgctgacc gcctggcgca tgcgctcccc
     6781 gatcacctcg gcgccgtcct gcttgcgtcc ctgcagccgc gactccagcg tgtcgcgcga
     6841 cggcggcagg atgtagatcg actgcgcggc gcgaatgcgc tcgcgcacct ggcgcgcacc
     6901 ctgccagtcg atctcgagaa tcacgtcctt gccgcccgcc agtgtctcca tgacccagct
     6961 gcgcgaggtg ccgtagaggt tgccgaacac ggttgcgtgc tcgaggaagt cgccgcgccc
     7021 gagcatctgc tcgaattgtt cgcggctcgt gaagtggtaa ttgaccccct ccacctcgcc
     7081 cgggcgccgc gcgcgcgtgg tgtgcgacac ggatgccagc aggtcgggat cctgccccac
     7141 cagagcggcg accaggctgg tcttgcccgc gcccgaaggc gccgacaccg tgtacagggt
     7201 tcctggcgcg gtcgtgttca ttcgatgttc tgcacctgtt cgcgcatctg ctctaccagc
     7261 accttcaact ccacggccga gcgggtcgtt tccgcggaag cggacttgga ggacagcgta
     7321 ttggcctcgc ggttgaactc ctgcatcagg aaatccagcc gccgtccgca ggccgcgccc
     7381 gcggccaggc tgtggcgtgc ctcggcgaca tgggattgca gacggtcgag ttcctcggcg
     7441 acgtcggatc gcgcggccag cagcgcgatc tcctgctcca gccggcccgg ctcgacctcg
     7501 acgcgcagtt ccgccaggcg gtcgacgagc ttctgcttct gtgccgcgag gattccgggc
     7561 agcacggcgc gcaccgaggc cacgatggtc tcgatgcccg cgaggcgttg ctcgatggcg
     7621 ctcgccaggc tcgcgccctc gcgtgcgcgc atcgcgatca attcgtcgag cgcctggcga
     7681 aacaccgcga gcgcactgcg cgctatctcg tcctcgtccg gatgcgattc cgcgatcacc
     7741 cccggccagc gcagcaggtc cagcacgccg ggacgcccga ggccgccggc cagcgcatcg
     7801 acccgggcgc atgcctcgtg caggcttcgc accaggtcgt cgttgatggc gatgggcccc
     7861 gtggcggacg gtccggcctg caggcgcagg atgcagtcga ccttgccgcg tgccagccgc
     7921 tcgcgcagca gttcgcgtgc tggcatttcc acctggcgca gggtctcggg caggcgcaac
     7981 tgcgcctcga gatagcgatg gttgacggag cggatctccc acgagagggt gccccacgcg
     8041 aacgacgctt cgcggcgggc gaacgcggtc atgctctgga ctgacggcaa tgctcgactc
     8101 ctgtgactgg cggccattgt agccctgcct cggggataaa tcatggccat gcacgcgtgt
     8161 gtataatcgg cgccccgcgc acccgcaaac acccatcgag gatcatttat ggagcgaccc
     8221 agcggccgcc gtccggacca gcttcgcccc gtgcgtttca cgcgcgccta cacgcgccat
     8281 gccgaagggt cggtgctcgt ggagtacggc gacaccaagg tcatctgcac cgccagtgtc
     8341 gacaagggcg tgccgcgctt catgcgtggg cagggtcgcg gctggctcac cgcggagtac
     8401 ggcatgctgc cgcgctcgac caacacgcgc atggaccgcg aggcggcgcg cggcaagcag
     8461 ggcggacgca cggtcgagat ccagcgcctg atcggccgct cgctgcgctc ggcgctggac
     8521 ctgcgcaagc tcggcgagaa cacaatcgtc atcgactgcg atgtgatcca ggccgacgga
     8581 ggcacgcgca ccgcgtccat caccggcgcc tgtgtggcgc tgtgcgacgc gctcgccgcg
     8641 gcgaagcgcc gcaagttgat caccgaggat ccgctgctgc acctgatcgc atcggtctcg
     8701 gtcggcatgt acaacgggca tgccgtgctc gacctcgatt acgcggaaga ctcgagcgcc
     8761 gagacggaca tgaacgtcat catgaccgag cagggcgatt acgtggaggt gcagggcact
     8821 gccgaggcca agcccttcaa ggccgaggaa ctgcagcaga tgctggatct cgcgcgccgc
     8881 ggcaccgaga acctggtgcg cgtgcagaaa atggcgctga gcgcgccact gcccgcgaaa
     8941 tagcgcggac agcgggctgc tagagtctct cgagctcgat gtcgtagtcc atgataagcg
     9001 gcgcgtgcga ggagaattcc tgcaccttgt acagcgcgcc gtactcgatg gttccgcgca
     9061 ggcccgccga gacgatctgc aggtcgacgc gccagccatc tcccgcggcc cgcccgcccg
     9121 gccaccagga gaactcgtct tcgtcctggt tcacgacgcg gaacgcatcg acgtaaccca
     9181 gctcgccgta caggccgtcg aggtactggc gctcggccgc gagaaagccg ggcgtgtcgg
     9241 cgtgcaccgc agcctgctgc acgtcgcgcg cggcgtgcgc catgttccag ttgccgcaca
     9301 gcacgtactg gcggcgcttc ttgcgcacct tgtcgagttg cgtgcgcagt tgctcgtaga
     9361 agcgcgactt gcgcgccagc gagcccgtgt cttccatgct ggccaccggg ccgagcagac
     9421 aggccacgct gatgttctcg aagtccgcct gcatgaagcg cccctcgcca tcggcctcgc
     9481 cgagacccag gccggtcatg atcgccttgg gcagtttgcg gcaatagatg gccacgccgt
     9541 tcgtggtgcc gtcgatcgcg tcgaaaaaat agctgaagta gccgtcggga ttgtatttcg
     9601 ccgcgcccag gcccgattcg ggtgcgcgca gatcctgcac gcagatgatg tccgcgtcct
     9661 ggtcgacggc ccagtcgtag aagcctttcg cggctgcctc gcggatgcca tccgcgcaga
     9721 aactgatgac gcgcatgttt ttccctgcct ttatacgccg cggcatgata catgagcgtc
     9781 ccggttcggg taaaaagcat gtttccgact ttgtgcgcgc tttgcgcgat gcggcgctga
     9841 tatacttccg tgtgccccga aacagtgcgt tttccgacat gtccgcgacc ggtttccgca
     9901 acgagttcat cgccctcgcg ctgcgccagc gcgcgctgcg cttcggctcg ttcacgttga
     9961 aatcgggccg caccagtccc tatttcttca atgccggcga attcagcagc ggtggcgcgc
    10021 tcgccgcgct gggccgctgc tacgccgcgg ccattgcggc ggccggcgtc gagtacgaca
    10081 tgctcttcgg tcccgcctac aagggcatcc cgctggcggt ggccaccgcg gtggcgatca
    10141 gcaacgacag cgggcgtgac gtgccctgcg ccttcaatcg caaggaagcc aagacccatg
    10201 gcgaaggtgg cacgctgatc ggcgccccgc tcgcgggcag ggtgctgatc atcgacgacg
    10261 tgatcacggc gggtaccgcc gtgcgtggcg tggtcgacct gatcgccgct gcgggcgcca
    10321 ccatcgctgg cgtcgtgctc ggactcgatc gcgccgagcg cggatcgggc gcggattccg
    10381 cggtcaccga gctggcgcgc gagctcggcg ttccggtctt cagcatcgcg cggctggacg
    10441 acatcgtgcg attcctcgag gagtccgaag gctttggcga gcacctgccg cagttgctcg
    10501 cctatcgccg gcaatggggt gaagcgggcg cctgagcccc gatcatcgtg gcagctgcta
    10561 gactcgttgc agcaatcacc ctgcgcaacg aggatcgcat gacttccagg ccgttccacc
    10621 gcgacgttcc ggctctaccc cgatggctgg gcgtggttgc attgctgtac ctgctcggcg
    10681 cccccgcctc cgcgctcgcc gaggatcggt tctacaagta cgacaatgcc gagggcatcc
    10741 cggtcatcga cgaccgcgta ccgccggagc tcgcccacaa ggggtacacg gtgctcaacc
    10801 gcgcggggcg cgtggtcgag gtcgtgccgc gcacgctgac cgcggcggag cgcaaggcca
    10861 atcccggcgc ggtgcaggcc atgctgcgtg cggaagaggc cgagcgccag cgccgctacg
    10921 acgaggcgct gctcgcgcgc tacagctcgg tggccgacat cgaggatctg cagcggcgca
    10981 aggtcaacga ggtgcaggtg cggatcaacc tgcagaaagg ctccatcgcc gcactcaagc
    11041 agcagctcga gagcgagcac gcgaaggccg cggacctcga gctcgccgag gcgccggtgc
    11101 cggcggacct gcagaagaaa atagaagagc tgcgagcctc cattgcggag gaggaagcgc
    11161 gaatcgccag gctggagcag gaccggagca cggtccaggc gcgctaccag ttcgatatcg
    11221 agcgcttccg cgccattcgt cccggtagcc gctgagcggg tctcagtccc cggcgcggcg
    11281 gaacaacgag ccggtcagca cgcgccactg ctcggcccag taatcggtgg gcgagcggcg
    11341 gaactccgag cgcacgtact ggatgatgcg tccttccgcg gtggcaagca gcaggttcgc
    11401 cgccaggttg accggcagcg ccgtgcgcag cccctgccgc acttcagcct cgcgcaggat
    11461 ctgtttcagt tcggtctcca gccggtcgaa cagctgctgc acccggccgc gcaggcggtc
    11521 cgtctcgccc gtgagcgcat cgccggtcag cagtcgggtg attcccgggt tgcgctcgca
    11581 gaaggtcagc agcagggtca ctatctgttc gcactgcgcg gcggcatcct gctcctcttc
    11641 gcggatacgc gaaatgcggc tgaaaagggt ggtctcgatg aactcgatga gcccctcgaa
    11701 catcttcgcc ttgctgggga agtgccggta gagcgcggcc tcggatacgc cgacgtcggc
    11761 cgcgagcgcg gccgtggtga tgcgcccccc gggtgtggct tcgagcatgt gcgccagcga
    11821 ctgcaggatc gcgtcgcggc gcgaggtctt gttattcatg tcgacgcggt agtccccggg
    11881 atgcgcaggg ccgtcatgct agcgattcgc tcaggcaccc gcaacgacag ggggcttgcg
    11941 gttgcagatc aacgtgccca cgccctcgtc ggtgaacacc tccagcagta ccgcgtgcgg
    12001 caccgtgccg tcgatgatgt gcgcgctggt gacgcctgat gctaccgcat cgagcgcgca
    12061 gcggatcttc ggcagcattc cgccccggat caccccttcg tcgatgaggc ggttgacctc
    12121 gtcgcggtcg agcccggtga gcaccttgcc ctcggcgtcc atcagccccg gcacgttggt
    12181 cagcagcatg agcttctcgg cctgcagaac ctcggcgatc ttcccggcga cgagatcggc
    12241 gttgatgttg tacgagagcc ccgccttgtc gacgccgata ggtgccacga ccgggatgaa
    12301 atcgctgtgc aggaacatgt cgagcaccgc gcggttgatg cggcgcactt cacccacgtg
    12361 accgatgtcc acgatttccg gggcgctggt ttccgccgtg cgcacggtga ccgacatgcg
    12421 cgatgcctcg atcaggtggc cgtccttgcc ggtgacgccg atcgcgttgc caccgtggcg
    12481 gttgatgttc gagacgatct ccttgttgac cgtcgcgccg agcaccatct cgaccacgtc
    12541 catcgtctgg gtgtcggtga cgcgcatgcc ctcgacgaag cgcgattcga tgttgagctt
    12601 ggccagcagc gagccgatct gcgggccgcc gccatggacg accaccgggt tcatgccgac
    12661 cagtttcatc agcacgacat cgcgcgcgaa gctttccttc agttgcgggt ccaccatcgc
    12721 gttgccgccg tacttgatga cgatggtctt gccgatgaag cgctggatat agggcaggct
    12781 ctcggtgagc acgcgggcta tgttcactgc ggcttcgcgg ttcagggaca tcggcgggat
    12841 ccttgcgcta cggatgtggt gcggcatctt aaacgcagcc acgggcgcgg gcgcgcaccg
    12901 cgggaccgtc gtgctaaaaa ctcacattcg ttccgggcaa aagacgccgg atctccgcga
    12961 gaaaggcggc ctggatgcgc gccagcgtgg cctcgtcgcg cccctcgaag cggcagatca
    13021 gcgccgccga ggtgttcgag gcgcgcacca gcccccagcc gtcggcgaat tcggcgcgca
    13081 cgccgtcgat gcggctgagg cgggcatttt cgagctcgag cgagaagccg cgggagatcg
    13141 cttccatggc cgcgaacttc tgctcctccg gcatctcgat gcggatctcg ggcgtcgcca
    13201 cgctgtgcgg cagttgcgcc agcaactcgt cgagcgtgct gctcccggcg ccgagcagtt
    13261 cgagcaggcg cgccgcgcag taaagggcgt cgtcgaaacc gaaccagcgg tcgcgaaagc
    13321 agatgtggcc ggtgaactcg ccgccgagca gcgctccgct ctccttcatc ttttccttga
    13381 tccaggagtg gccgctgcgg cacatgatcg gcctgccgcc gttctcgcgc accaccgccg
    13441 cgaggtcacg gctgcacttg acatcgaaca ccacgtccgc gcccggatgg ctcgcgagca
    13501 cgtcgcgcac gagcagcatc atgaggcggt cggcggtgac gatggtgccg ctcgcactga
    13561 ccacgccgat gcggtccgca tcgccgtcga aggcgatacc cagcgccgca ccggtttcgg
    13621 ccaccttcgc gatcagggcc tgcaggtttt ccggcaccgc gggatccgga tggtggttgg
    13681 ggaaccggct gtcgagttcg cagaacagcg gcacgacatc gcagccgagc tcgcggaaca
    13741 ggtcctcggc aatcaccgag gcagcaccgt tgccgcagtc gatcaccacc ttgtgcctgc
    13801 catcgacgag cacgtcgtcg gcgaccgcct tgatgtagtc ggcgtcgatc tcggtgacgc
    13861 ggtagctgcc ctgcccctcg tggaacaagg cgtgctcgat tcgttcgcgc aggcgcagga
    13921 tgcccgcccc gcgctgggtt tcgccgccga tcatgatctt gaagccgttg tgctcggccg
    13981 gattgtggct gccggtgatc atcacgccgc tcatcgtgcg cagatgatgg caggcaaaat
    14041 agagcatcgg cgtggcgacc gtgccggtgt cgatcacatc gatgccggtg gacagcaggc
    14101 cgttgacgag atgttcgcgc agccgcgggc tgctgtcgcg cccgtcggca ccgaccatga
    14161 tcgacgtgca ttgccggtcc agcgcttccg agccgatggc ctggccgatc gcatgcgcca
    14221 gttgcgacgt gatctgcgct gagtgcccgc ggatgtcgta gtcgcggaat atctccgcgg
    14281 gaatgtccgg cggcgcctcc tcctccccgg tctcggccag ctgctcgacg atctccagcg
    14341 gatccggcgc cgggcgcgcg gggcgtgcag caaggggggc gggttccgcg gtgccgggct
    14401 gctggtcgcg gcgcaactgc accagcgctt cgccgagtcc cgcgagttcg tgccaggaca
    14461 gtgccggcag cgtcgcggtg gtattgcgct ggcgcagggc gagatccgtc agtttcgccg
    14521 cgtcatcgtg cagtccggcc gcgagggcgc ggaaggtcct gcgtaccagc gcgatcagca
    14581 gcaaggccgc ggcgccggcg cccagggcca gcgccgtgat gccgagcgac agtcgcagcg
    14641 gcacccgcga cgccggttcg aaggccgcgt acagcgggcg aatgccggtt gccgcgcggt
    14701 aggacggctc ggtgtagccg tctgccctgc ccgttcgcac catgggcagg tagttcccgt
    14761 ccgtgccctg cgaccacacc gagaactcgc ccgattcgtg cgccagcggc aggtgatcgc
    14821 gcaccaccgt ggcgaggtcg gcgcgcgcca gcaccacgcc gccggcgtgg tctgcggacc
    14881 ccggcacagc cgctgccgcg accaggatcc agccgccatt gtcgcgatag gcttccgtgg
    14941 caggattctc gccattgaag gcgcgaccgg cgaggcggat ctcgatgttg ttgcgcaaga
    15001 cctccttgcg aaaggcgggg tcggcgatcc ccagcgggcc gagcgggatg aactgcacgg
    15061 attgcacgcc cgggaacgcg cttcgcggcg cgcgctccgg tgcgccggtg gcgtcgtgcg
    15121 ccagtgcctg cgcgtgcgcc gccagcaggt gtccgtaggt tgaaatcgac agcgccgcct
    15181 ggcgcgccgt ggcctgggcc cgttcgcggt gggcgagcag ttccgtggtc cagctgtaag
    15241 cgacggcagc cgccagcagc agtaccgaga cgatcccgat cgcgcgtacg acaagccgcc
    15301 gggcgggcgc gagtccggtg ctcgggacgg cggaagtcac gaagcctcgc ccgcggccgg
    15361 attgccttcg cggaagcggg cgcctattgc ggcgaccagc gcgcgcgcga tctgggcctt
    15421 ggttccacgc gccagctcgg tggtgtcggt ggccgagatc aggcagacct cgttgaactc
    15481 gctgttgaag ccgatgtcgc cgcgcgcgac gtcgttggcg acgatcaggt ccaggcgctt
    15541 cgcggcgagc ttgccgcgcg cgtaggcgac gacgtcggtg gtctcggcgg cgaatccgac
    15601 ggtgaacggg cgtgactcca gcgccgcgac atcggcgacg atgtccgggt tgcgcaccag
    15661 ccgcacctcc atgcgctcgg tggacttctt gatcttctgc ccggcgcagt gctcggggcg
    15721 gtagtcggcg acggccgcgc aggcgatgaa gatgtcgcat tgcgccgcgc ggctcatcac
    15781 ggcctcgtgc atctccatgg cgctggaaac gtcgatgcgc gccacgcgcg ccggggtgtc
    15841 gaggtgcacg gggccgctga ccagggtcac gccggcgccg gcctcggcgg cggccgcggc
    15901 gatcgcgaaa cccatcttgc cggaactgtg gttgctgatg tagcgcaccg ggtcgagggc
    15961 ctcgcgcgtg gggcccgccg tgatcatcac gcgcacaccg tcgagatcgc gtggcgcgaa
    16021 gcggcttgcc agtgcggcga gaagctccgc gggttcgagc atgcgccccg ggccggtgtc
    16081 gccgcaggcc tgcgagccgc tgcccgggcc gagcaggtcc acgccgcgtt gcgtgagcac
    16141 gccgcagttg tgccgggtgg ccgggttgcg ccacatggcc tggttcatgg ccggcgccac
    16201 caggaccggg gcgtcggtcg ccaggcacag cgtggcgagc aggtcgtcgg ccagcccgtg
    16261 cgcgagtcgt gcgatgaaat ccgcgctggc cggggccacg accaccaggt cggcccagcg
    16321 cgccagctcg atatgcccca tcgcggcctc ggctgtctcg tcgagcagtt cgcgatgcac
    16381 cgggttgccc gacagggcct gcatcgtcag cggggtgatg aattcgcagg cggcgcgcgt
    16441 catcaccacg cgcaccacgg cgccggcgtc gcgcaggcgc cggaccagct ccgcggactt
    16501 gtaggcggct atgccgccgg tcacgccgag gagtatctgc ctgttgttca gtgtcgccat
    16561 gggccggatt tatcaggatg agggcgtgcc gcgttgccgc gggcggttca gctttccgcg
    16621 gcattctatt atagtcagat gagactcatg cgacgcgtgg acagggaggt tcgcgatgac
    16681 catcaagagt tggcccgccg ccgagcgccc gcgcgagcgt ctgctcgggc gcggagcaag
    16741 caccctcagc gatgccgaac tgctcgcgat actgctgcgc accgggctgc cgggcggcac
    16801 cgcggtcgat cgggcgcgtg agttgctggg cgccttcggc gatctgcgcc agctgctcga
    16861 cgccgagcct gccgcgttct gcgccacgca cgggctcggg gaggcgactt acgcccacct
    16921 gcaggcagcg ctggagctct cccgccgtta cctgctgagc cggatcagtc gcgggcaggc
    16981 attgggctcg ccgcaggaag tgaggcaatt cctgcagctg aagatgcgct gcctgccgca
    17041 cgaggtgttc gcctgcctgt ttctcgacaa ccagcacacc gtcatcagct acgaggagct
    17101 ctttcggggc accatcgacg gcgccagcgt gtatccgcgc gagatcgtga agcgctcgct
    17161 ggcgctgaat gcggccgcgg tgattttcgc gcacaaccac ccttccggcg tcgcggagcc
    17221 ttcgcaggcc gaccagcggc tcacccagcg cctgaagagc gcgctcggca ccgtcgacat
    17281 ccgggtgctg gaccacttcg tggtgggcga gtctgaggcg ctgtcctttg ccgagcgcgg
    17341 tctgctgtga gtaaaaaata cccgatcgcg gctgtcgcct tgtacgcccc gaggccttct
    17401 gatataaagg cgcgctcttt tcggcaggcg tccgtctcgg ccgcgtgcat cgagccatag
    17461 ttttcgagag gcccattcca tgtccaaggt gtgtcaggta accggcaagc gcccggtagt
    17521 agggaacaac gtatcccatg ccaacaacaa gacgaagcgc cgcttcgagc ccaacctgca
    17581 caagcaccgg ttctgggtcg aggccgagaa gcgtttcgtg acgctgcgcg tatccgccaa
    17641 ggccatgcgc atcatcgaca agctgggcat cgacagcgtg atcgcggaca tgcgcgctcg
    17701 cggcgagcgg gtctgaagga aaccaaggag taagcagcca tgcgtgacaa gatcaaactg
    17761 gtttcgagcg ccggtaccgg tcatttctac acgaccgaca agaacaagcg cacgaccccc
    17821 gacaagctcg agttcaagaa gtacgacccc gtcgtgcgca agcacgtggc gtacaaggaa
    17881 gccaagatca agtgagtgcc gcccggcact gacacaaacc cggctgcggc cgggtttttt
    17941 gtttgggtcc ggccggcggc cgcaccccgc ccgcgaggct gcgtggatcc atgcccgaac
    18001 tgcccgaagt cgaaaccacc cggcgcggca tcctgccctg cctgagcgga cggcgaatcc
    18061 gcgcgctcga ggtccgcgag ccgcgactgc gttggcccgt gccctccgat ctcgatgcga
    18121 tcctgcgcgg tgaggagatc gacacggtcg agcgccgcgg caagtaccta ctggtccggc
    18181 tcggcaaggg gaccctgctg atccatcttg gcatgtcggg cagtttgcga gtccttactg
    18241 cgcccgcagc cgctccccgt aagcatgatc atatcgacct cctgctggag gacggccagt
    18301 tgctgcgcta tcacgatccg cgccgtttcg gtgccttcct gtggctggag ggcgacccgg
    18361 ccgcgcatcc gctgctggcc gggctggggc ccgagccgct cggcgccgga ttcgacggcg
    18421 actacctgtt ccgcctgtcg cgcgggcgcc gcaccagcgt gaagcagttc ctgatggatg
    18481 gccacgtggt ggtgggggtc ggcaacatct acgccaacga agccctgtac ctggccggca
    18541 tccatccgcg gcgacaggcg ggccgcatcg cggccgcgcg ctacgcgcgg ctggccgatg
    18601 cggtgcgccg ggtgctcgag ggtgcgattg cgcagggagg gacgacgctg cgcgactatg
    18661 tcaacggcaa tggcgtgccc ggctatttcg cgctcagcct gagcgtttac gggcgtacgg
    18721 gccggccctg cccgggctgt ggcgaggcga tacgccaggc gcgcaatgcg cagcgcagca
    18781 cattctattg cccccgctgc cagcgctgag cacggcgtat gcaaaaacgt ctattcttag
    18841 cggcaccgtg cgccatggcg cgcgtccatg tggcacaagt tcctgcaacg gagacggaga
    18901 tgaaacattc caaggcttcc cggctggcgg tcgcaaccct gggactggtg ttgatgacgc
    18961 cgtggttcgc caacgcgcag cccgttgacg agaatccgag cgcgctcgcg atgattggtg
    19021 acctggtcat tgcccgcccg ctgctcgtcg ttgccacggc ggccggcgcg gcggcattcc
    19081 tcgtgtcgct gccgttcacc gcgaccggcg gcaacatgga ccaggcagcc gacacgctga
    19141 tggcaggtcc cgcgcaagca gcctttgtac gctgcctggg ttgcaggagc agcggccggc
    19201 gacaggcggt ctccgactga aaccgggtca gtgcgagccg cacaggcgct cgaacttcag
    19261 cagcaattcc tggcggctct ccggatgggc cggatccagc gggatgcagt ccacgggaca
    19321 caccttgcga cactgcggct cgtcgaaatg ccccacgcac tcggtgcagc gcgcggggtc
    19381 gatctcgtag atttcctcgc ccgggtagat cgcctcgttg gggcaaacgg gttcgcatac
    19441 gtcgcagttg atgcactgcg tcgtgatcaa cagggccatg gcgcccgccc ttcaggccgg
    19501 atcggcaaat ttctcgagca gggcggcgcg aacgcgcgga tgcacgaact tggtgacgtc
    19561 gccctgtagc gaggcgatct cgcgcaccag cgtcgaggag ataaaagaaa ggtggtctgc
    19621 cggcgtgagg aacacggtct cgaagtccgg gtccatcgcg cggttcatgt tggccagctg
    19681 gaattcgtat tcgaagtcag acaccgcgcg cagcccgcgc agtaccgcgc agcccttgcg
    19741 ctcgcgcagg aacttcacca ccaggatgtc gaagccgacg atctcgacat tctgcaggtg
    19801 tccgagcgcg gcctggcaca ggccgatcct ctcgtcgagc gagaacagcg ggcgcttgcg
    19861 ctcgctgctc gcgatcgcca ccaccacgtg gtcgaacagg cggcaggccc gctccaccag
    19921 gtcgacatga ccgatggtga tcgggtcgaa ggtgccaggg tagacgatcg ttcgcatgtg
    19981 cagaccgtgc gcaatcaggg gcgggggccg catggtagcc aaatacccgc ggcgcgacaa
    20041 atattgcccg gcaaagttcc cccctgccgg cgagcggcgc tcagcccggg gccaggtaaa
    20101 gacggtaggc cacctgcccg gcgcatttct cgcggtgcaa cagccagccc gggggcagtt
    20161 cgggcgcggc ttcagcgacg ccgcattcga ggtacacccg cgcgcctggc gccagcagcg
    20221 ccgagcccgc cagcaaccgc aggcagcgct gtgccgtgcc gctgccgaac ggcggatcga
    20281 tgaaaacgat gtcgtagcgg cgatcgcgca ccgtttcgat ccacgccacg gcatcggctg
    20341 ccaccagctc ggcagcgctc gtgccaagcc gttccaggtt gtggcggact tcgcgcagta
    20401 ccctcgcgga aaggtcgacc agcgtcgcgc ccgctgcgcc acgcgacagc gcttcgatcg
    20461 ccagcgcccc gctgccgcag aacaggtcca ggcagcggct gccaggcagc tgcggttgca
    20521 gccagttgaa cagggtttcg cgcacccggt ccggggtcgg tcgcagtccc ggctcggcct
    20581 ggaactcgat gcgccggccg cgccagctgc cgccgatgat gcgcacctgg ctgcgcgagg
    20641 cgtgtccagc ggcgggggcc ggtttgcgtg ctgccatacg gtgagcgggg ggaagtgaaa
    20701 agacgggccg aaggataccg tataatcgcg cccctttccg gatggtggcc ccgaaccgtg
    20761 gtgctcagat tcttttcacg caagaaaccc gacgccgcgg cgccggacgc ggcagccgcg
    20821 cccgtcgttg ttccggtacc ggttcccgag cccgagcccg agaccgagac cgagccccct
    20881 gcagcggcgc cgatgctcca ggcctcgcac gatgcacccc cggcgcagac cggtgacgcg
    20941 gcgcgcccgg gttggttctc gcgcctgcgg gccgggctgg gcaagacccg gaatgcattt
    21001 tccgaggggc tcggcacctt gttcctgggc cgcaagaccc tcgatgagga cctgctggag
    21061 gagctcgaga cgcggctgct gctcgccgac gtgggcatgc ccacggtgcg cctgatcatc
    21121 gatggcatgg tgcagcgcat caagcgcaag gagctcgacg acccccaggc gctgtaccgg
    21181 gcgctgcagg agcaactggt cgcggtgctg gcgccggcca gtgtgccgct cgcgatcgac
    21241 accgcgcgca agcctttcgt catcctgatg gttggcgtca acggagccgg caagaccacc
    21301 acgatcggca agctggcgcg gcgctaccag gccgagggca ggagcgtgat gctggccgca
    21361 ggcgacacct tccgtgccgc ggcggtggag caactgcaga tctggggcga gcgccacggc
    21421 gtacccgtgg tggcgcagcc gaccggttcc gacagcgcct cggtgatctt cgacgcgttg
    21481 cagtccgcgc aagcgcgcgg ttgcgatgtg ttgattgccg ataccgcggg tcgcctgcac
    21541 gtgaagggca acctcatgga agaactgcgc aagatcgtgc gcgtgatgca gaaagtcgat
    21601 ccgggcgcac cgcacgaggt gatgctggtg ctggacgccg gtaccgggca gaacggcctg
    21661 gcgcaggcgc gccatttccg cgaggccgtt ggcgttagcg ggatcgcgct gacgaagctc
    21721 gacggtaccg ccaagggcgg catcgtgttc gccgtggcgc gcgaactcgg cttgcccatc
    21781 cgctacatag gcgtcggcga gagcgccgag gacctgcgcg agtttgaggc gcaggagttc
    21841 gtggcggccc tgttcgacga ttccgcgcgc ccctgagggc gggcgggcag tggaactttt
    21901 gcccgcttag cactctaacc gctcgagtgc taaaatgcgt ggccatgaca ggaggtagat
    21961 atatgagcaa ggaattgcag ccgctgatgg cactgacgcc cggtggcaac atcaatgcgt
    22021 acatccaggc tgtcaacgcg tttccggtgc tgacgccgga acgggagcag gcgctggggg
    22081 aggcgctgta ttttcgcgat gatctggccg cagcgcgtga gttggtgctc gctcacttgc
    22141 gtttcgtggt gcatatcgcc cgctcgtatt cgggctatgg cctgccgctg gccgatctgg
    22201 tgcaggaagg caacgtcggg ttgatgaagg ccgtgcgtcg cttcgatccg cagaagggcg
    22261 tgcgcttggt gtcgttcgcc gtgcactgga tcaaggccga gatgcacgag tacatcctgc
    22321 gcaactggcg catcgtgaag atcgccacca ccaaagccca gcgcaagctg ttcttcaacc
    22381 tgcgtagcgt gaagaagagc ctgggttggc tgaccaacga cgaagccgcg gcgatggcgc
    22441 gcgatctcgg cgtgggcatc gacgaggtcc ggcgcatgga aggccggctg gcctcggccg
    22501 atgtcgcctt cgatgccggc accgacgatg acgacgatgc ggcgcagttc gcgccggtgc
    22561 actatctcga ggaccgccgc ttcgatcccg cgcgcgagct cgaggacagc gactgggaag
    22621 cgtccagcca ggagcggctg gagcatgcgc tggagggcct cgacgagcgc agccggcata
    22681 tcgtgcagag ccgctggctg aacgaggaca aggccacgct gcacgaactc gcggcccgct
    22741 acggtgtgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcggcgcttg
    22861 ccgtgtgggc taccgccgcc gactaccaga tgtaccactc gctcgccctg ctggcgctgg
    22921 gcctcgcgcg ccctgccgcg ctggcaccgc gctggcgccg cgtcgcagtc gcctgcttcg
    22981 cggttggcat cgtgctgttc tcgggcagcc tgtacctgct cgcgctgagc ggcatccgcg
    23041 ccctcggcat gatcacgccg ctcggcggcg tgctgttcct gctgggctgg gttgccgttg
    23101 ctgcaggagc gtggcgcagt gagcactgac gaacaagagc tgcgcgaacg ccccatccgc
    23161 agtttcgtcc gccgccaggg ccgcttcacg ccggcacagc gcgatgcctt cgactcgctg
    23221 tgggcagggc gcgggctgga gcccggcgat accctcctcg acctgccgtt gctgttcccc
    23281 ggttgcacgg cattcgtcgt cgagatcggc ttcggcatgg gccacacgct ggtggagctc
    23341 gccagccgct cgccgcagac cggcttcgtg ggcatcgagg tgcaccgacc tggcgtcggc
    23401 aagttgctcg cgatggccga ggagcgtggc ctgcagaaca tccgcgtttt ctgccacgac
    23461 gcggtggagg tgctgaaccg tgcgatcccc gacgcctcgc tcgacgggct gctgctgttc
    23521 ttcccggacc cctggcacaa gaaacgccac cacaaacggc ggctggtgca gccgccgttc
    23581 gttgcgctgg tgcagcgcaa actgaaaccg ggcggcctgt tccaccttgc gaccgactgg
    23641 gaggactatg cgcggcagat gctcgaggtc gtcgagtccg tgcccggctg gctcaacctc
    23701 gccggcgcgg gatgcttctc gccgcggccc gaatcgcgtc cgctgacgcg cttcgagcag
    23761 cgtggcatgc gcctggggca cggggtgtgg gacctgctgt tgcgcaaggg cgaagatcag
    23821 gcgaactccg cgagcaccgc gttcaggtag cgaaagcccc gggccgtggt acacaggcgc
    23881 gccgggtcag tttccatcag gccgtgcgcg cgcagcgcgc cgagttgggc gccgatcgtc
    23941 gccgcggcga gcccggtgcg cgcttcgaac tgcgcgacgc tcgcgccttc gcgcaggcgc
    24001 agcgcattca tcaggaactc gagcgcgagc tcgtcaggtg ccacggcggt gtcggtgcgt
    24061 atcgacgtcg ccgctcccgc gaggtagtcc ttgggcacgc gcgtcttgcg cgtgcgcagc
    24121 acgcggccct gcgccggcag cgtgagcttg ccgtgtgcgc cggcgccgat gccgatgtag
    24181 tccccgaagt gccagtagtt caggttgtgc cggcattggt ggccgggccg cgcccaggcg
    24241 gagacctcgt actgctcgta gccgtggcgt gccagcagtt cctcgccccg gtcctggatc
    24301 tcggccagct cgtcctcggc aggcagcggc ggcggcgcgc tgtggaaggc cgtgttcggt
    24361 tccaccgtga gctggtacca ggacaggtgg ggggcctcgt gcgcgattgc ctgctcgagg
    24421 tcggccagag ccatcgcggg cgactggtgc ggtagtccgt gcatcaggtc gatgttgaag
    24481 ctcgcaaagc cggcgtcgcg cgcggctgcg atcgccgcgt gcgcctggcg tgcgtcgtgc
    24541 acccgcccca gggcgcgcag gcagccatcc gcgaagcttt gcaccccgat cgagaggcgg
    24601 ttcacgccgg cggcgcgata gccgcgaaag cgcgcggcct cggcgctgcc gggattcgcc
    24661 tccagcgtga cctccgcatc cggcgccagc gcgagccgtt cgcgcacccc ggcgagcagc
    24721 aaatccacca cctcggcggg gcacaggctg ggcgtgccgc cgccgatgaa gatcgagccg
    24781 gcctcgcgcc cggccgcgaa gccgcgatcc tgcccgaggt cttccagcag gcgcgccgcg
    24841 taggccgcga acggcggttg cgcctcgcgc tggtaggaat tgaagtcgca ataggggcat
    24901 ttgcgctcgc accacggcag gtggatgtag agcgcgagcg gcggcagcgt cagcacggtt
    24961 tgccgagggc gagcagcagt tgcccgagcg cctgcgcgcg atggctcaag cggttcttga
    25021 gctcgcgcgg cagttgcgcc gacgagcagc cctcggtctc gaccaggaac agcgggtcgt
    25081 agccgaagcc gttttcgccc gccggcgcct gcagtatccg cccctcccag cgtccggcgc
    25141 agatcagcgg caccgggtcg gcggggtggc gcagcagcgc gagcacgcag tgaaagcgcg
    25201 cggtgcgccg cgccgcgggc acgtcggcga gcgcctcgag cagcttgcgg ttgttggccg
    25261 catcatcgca gggctccccc gcgtagcgcg ccgagtgcac gccgggctgg ccggccagcg
    25321 catcgacctc gaggccggaa tcgtccgcca gtgccggcag gccggtgtgg gcgctcgcgt
    25381 ggcgtgcctt gatgatcgcg ttctcgacga agctgagccc ggtctcggcg atcccgggca
    25441 ccgcgaaatc ctcctgcgag cgcacctcga tgccgctgtc ggcgagcagc gcgtgcagtt
    25501 ccgcgagctt gccgcggttg ccggtggcga tcacgagttc gctcagccgc atcgttgctc
    25561 cctcagtcgg catagagcac gtggttgaac tgcagcacga gcggacgcgc ctcgcccgcg
    25621 ggctggatcg cgatgtcgaa gtactgcgtt tcgccggaga cgaagccgaa ctcggcgatg
    25681 tagtagacgg cctgatcctc gcggatctcg cgaaactcga gcggcgtctt gcggatcagg
    25741 tccccgcgcg tgccgctcag caccgcgctc acggtggccg cgtcccgcgt gccgcggcgt
    25801 accgcgatgt tgagcagcgc gcggtccttc gcccgcacga tgccgtaggc cgcggcgatg
    25861 tcgggctgca ggaagctgct ggcgaacacg ctgtaatgca cctcgaagct gccgtactgg
    25921 cgcatctcgg cgcgagttgg cgtcgcgagc agcgcgagca gcgcgagcag cagggagaca
    25981 gccagggtgg caacgggtga tcggcgcgat gagtgtttca tttggtcaca tggtagatgg
    26041 cggtgacgcc gcagagattg ggtagcaggc gcgcggccgg cgtggcgcgc tggttcgcgt
    26101 caacgacgac ccggttgatg acgtggatgt tcttttcccg gcacagcgcc tcgaaatcca
    26161 gcacggtgca gaagtgaatg ttcggcgtgt cgtaccaggt gtagggcatg aagcgcgaga
    26221 ccggcatgcg gcccttgccc gcgatctgca ggcggcagcg ccagttgccg aagttcggaa
    26281 aggtgatgat gcattcgcgc ccgatgcgca gcatctcgtc gaggatgatg tgcggatagc
    26341 gcaccgcctg cagggtctgc gacatcacca cggtatcgaa gctgtcgctg cggaaattgc
    26401 cgagcccgcg gtcgagatcc tgctcgatca cgttgacgcc acgctccagg cacagcgaga
    26461 tcttgatcgg gtcgatctcc agcccgtagc cgcgcacctg ccggtcctgc gtcaggtccg
    26521 cgagcagctt gccgtcgccg cagccgaggt cgagcacgcg cgcgccgggc ttcacccact
    26581 gctggatgat gtcgaaatca acgcgcatcg gcgacgctcc gtggggcaag gcccgcgtgc
    26641 acctgctcca tgtagcgctg cagtgcgtcg tggtagcgcg ggatcggcag caggaaggcg
    26701 tcgtggccct cgttggcctc tatctccacg tagctcacgt cgcggtgcgc cgcgagcagc
    26761 gcgtcgacga tctcgcgcga gcgccgcggc gagaagcgcc agtcgctgga gaacgagacc
    26821 accaggaagc gcgcgcgcgc gcggcgaaaa gcctcgaccg gatcgtcgcc gaagtcgcgc
    26881 gcgagatcga agtagtccag tgcgcgcgtc atcaacaggt aggtgttggc gtcgaaattg
    26941 tcggcgaact ggtcgccctg gtagcgcagg tagctctgca cctggaactc ggcgtcggcc
    27001 tcggatcccg gcgcgaagga gccgctgcgc agctcgcgcc cgaacttgtc gcccatcagc
    27061 tcgtcggaca gataggtcac gtggccgatc atgcgcgcga tcgccaggcc ctggcgcggc
    27121 agcgtgccgt gctcgaggaa ccagccttcg tggaattgcg gatcggccat gatggccttg
    27181 cgcgcgatct cgttgaacgc gaggttctgc gccgagagct tcagcgcggc ggcgatcacc
    27241 acgcagtggc gcacccgctc ggcatggtcg atggtccagc gcatggcctg catgccgccg
    27301 agactgccgc cgaccacggc ggcccagcgc tcgatgtgga gcaggtcggc cagccggcgc
    27361 tgtgtggcga cccagtcgcg cacgcgcacc accgggaagt ccggtcccca gatccggccg
    27421 gtcgcgggat tgacgctgtg cggcccggtg ctgccggcgc agccgccgat gttgttcagg
    27481 ctgaccacga agaaacgcga ggtgtcgatc ggcttgccgg gtccgatgca gttgtcccac
    27541 cagccgggct tgcggtccgc gggcgagtgg taacccgcgg cgtgggcgtt gccgctgagc
    27601 gcgtggcaga tcagcacggc gttggagcga tccgcgttca gcgtgccgta ggtctcgtag
    27661 acgatgtcgt agccaggcag cgtgcgcccg caggcgagct cgagcggctg ctcgaagcgc
    27721 tgcgtgcggg gcgtcacgat gccgacactg ccggccgctg ctcgcggttc catggtccgg
    27781 ggtccgttgt ggggcgggca atcttgcaac tgtgtgcttt cctgttcaag cgcgggccgg
    27841 cgcgatgccc agatccggcg ggatgcgcgc aggagcgcgg accagggcgg tcttgaagcg
    27901 gctgccgtgc ccggtgccga tttccaccgc gccgggcgcg acgccgaact cgctggcgag
    27961 gaagcggcac agccgctcgt ttgccgcacc ctcgctgggc acggcgctga tacggatgcg
    28021 caaccttccg ccgtgctcgc cgatgatccg gtcggccgag gcgcgcggct gcacctggca
    28081 gcgcaacgac agcacctcgc cctgccagcg ccagaacggc ccggtttccg ccatcagaag
    28141 cccagcacca cgccgcgcgg aagcccgacg ccctgtgcca ggccgcgcag caggatctcg
    28201 agcacgttga tgatgacgaa caccaggatc ggtgacaggt ccaggccgcc gagcggcggc
    28261 agcagccggc gcaccggcgc catcaccggc tcggtgagct ggtacagcag cgcgatcccc
    28321 ggatgatggc tctgcggcgc gatccagctg aaaacgatca acagcaggat cccgaagaaa
    28381 tagatgttca gcagcagcga caggatgccg agcagcgacc acgcgagcag cagcagcggg
    28441 ttgccgatcg aaaagccgag cagcgcgagc accaccagtg tcgcgatgca ctggatcgcg
    28501 agcgccagca ccaggctcgc gctgtccagg cgcccgagtg gcgggataat gcgccgcagc
    28561 ggccgcagca gcgggctcgt gaccttcacg atgaactgcg agatcgggtt gtagaagtcc
    28621 gcgcgcgcca gttgcagcag caggcgcagc agcaccgcga acagcagcac gccaaaggcg
    28681 gtctggacca ggtagcgaga gatctccgcg aaggttccca tgtgcgtcgt ggttcccggc
    28741 tcagggctgc ccaagctcgt cggcgagctc gcgcgagcgg ttgctggccg cctcgagcgc
    28801 ggattcgaag acgcggcgca ggccgcgctc ctccagcacc cgcagcgcgc gctcggtggt
    28861 gccgccgggc gaggtcacgc ggcggcgcaa ctccaccacg tcgacgtcgg tttccagcgc
    28921 catgcgcgcc gcgcccagcg cggtttgcgc ggtcagctgg cgcgcgagcg ccgggtcgag
    28981 cccgaggttc tcggcggcga gctgcatcgc ctccatgacc aggaagaagt aggccggccc
    29041 gctgcccgag accgcggtta ccgcgtgcat ggcgtcctcg tcctccaccc aggccacgaa
    29101 gccgacgctc gacaggatct cgtgcgcggc ggcgcgctgc tccgcggtta cggcgggcgt
    29161 ggcgtagagg gcggtggcgc tcgcctgcag cagcgccggc gtgttgggca tgcagcgcac
    29221 gatggcgacg tcggctccca gccaggactg cagcgaggca gtgccgatgc ccgcggcgat
    29281 cgagatgacc agcgagccgc gttcggccac ggccgcggcg atgtcgcggc aggcgctgcg
    29341 cagttgctgg ggcttcacgg ccagcaccac gatgtcggcg ctggcggcgg cttcgtggtt
    29401 gtcggcgctg gtgagcaccc cgagcgcgcc gaggcgctcg cggcagcccg cgtcggggtc
    29461 gctgacgcga atcgcgcgcg gatcccagcc gcgcgcgatg atgcccccga ccatgctgcc
    29521 ggacatgttg ccgccgccga tgaaggcgat gttgctgcgg gtcatccgcg tctccctgtc
    29581 atgcgttgaa tctccgggct catgtcccgc gcggcccgaa cagcgcggta ccgatacgca
    29641 ccagcgtcgc gccctcggcg atcgccgcct ccatgtcgtc gctcatcccc atggacagcg
    29701 tgtccagccc ggggatgtcg cgctgcagct ctgccaggca gcgcgcgagc cgcgcgaagg
    29761 gcaggcgctg cgcgtcgatc ccatccgacg gggccggcag cgtcatcagg ccacgcaacc
    29821 tgaggcgtgg cagcgcggca atcgcccgcg ccagcgccgc tagctcgtgc ggggcggcgc
    29881 cgcccttgct cgcttcgccg cacaggttca cctcgatgca gcaatcgagc ggcggcaggt
    29941 gcgccgggcg ctgctcgtcc aggcgctggg cgatgcgcag ccggtcgatg ccgtgcaccc
    30001 aggcgaagct ggcggcgatg gccgcggtct tgttgccctg gatgcggccc acgaagtgcc
    30061 atgttaacgg aagatccgcc agcgcctcga tcttgggcag cgcttcctga acgtaacttt
    30121 caccgaattg cgccagcccg gcgcgatggg cctcgcgcac gcgctccggg ccgtgcgtct
    30181 tgctgatgcc caccagcgcg accgagccgg gcgcgcgccc gtggcgcgcc tcgaggcggg
    30241 cgatctgccc gagcagggcc cgggttcgtt cggcgatcgt gtccatgcag tccctcgcgt
    30301 gcggcgcggc tacgctggcg gagccggccg gcccgtttcc tttaccgccc ggttgcgtgg
    30361 tctattatac cgaaggggcg tgacatgacc tgcgggatgt cgcgcatcgc ttcaccgtcg
    30421 ggaaagccca tggatatcac cgaacttctc gccttcgccg ccaagcagcg ggcctccgac
    30481 ctgcacttgt cggccggcct gccgccgatg atccgggtcg acggcgacgt caggcgcatc
    30541 aacctgcctc cgatggatca ccggcaggtg catgacctca tctacgagat catgaacgac
    30601 aagcagcgtc gcgactacga ggagttcctg gaaacggact tctccttcga ggtgcccggg
    30661 gtggcgcgct ttcgcgtcaa cgcgttcaac cagaaccgcg gctcgggcgc cgtgttccgc
    30721 accatcccct ccaaggtgct gaccatggag gatctcggga tggggcaggt gtttcgcgag
    30781 atctcgatgg agccgcgcgg gctcgtggtg gtgaccgggc cgaccggttc gggcaagagc
    30841 accacgctgg cggcgatgat cgactacatc aacgacaaca agtaccacca catcctgacc
    30901 atcgaggacc cgatcgagtt cgtccacgag tccaagaaat gcctggtgaa ccagcgcgag
    30961 gtgcaccgcg acacgcacgg cttcgctgag gcgctgcgct cggcgctgcg ccaggacccc
    31021 gacatcatcc tggtcggcga gatgcgcgac ctcgagacca tacgcctggc gctcacggcc
    31081 gccgagaccg ggcacctcgt tttcggcacc ctgcacacga cctcggcggc gaagaccatc
    31141 gaccgtatcg tcgacgtgtt tccggcgcag gagaaatcga tggtccgctc gatgctctcg
    31201 gagtcgctga tgggcgtcat ttcgcagacg ctgctcaaga aggtgg
//
LOCUS       DAORUQ010000013        30948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1017_length_30948_cov_5.887385, whole genome shotgun sequence.
ACCESSION   DAORUQ010000013 DAORUQ010000000
VERSION     DAORUQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 30948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30948
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_30948_cov_5.887385"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..792
                     /locus_tag="PLW13_02415"
     CDS             1..792
                     /locus_tag="PLW13_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HQY69249.1"
                     /translation="MAVARLYGEPLFAMPQDLYIPPDALEVFLEAFEGPLDLLLYLIR
                     RQNLDILEINVAQITEQYMQYVELMDAMQFELAAEYLVMSAMLAEIKSRMLLPRQQSA
                     AEEETDPRADLIRRLQEYEQFKQAAEDLDGLPRLERDVFQAAAEPPELRRSKPQPQVE
                     LRELLLAFGEVARRASMNVSHHVQMERLSTRERMVEILARLHTPHFVPFVSLFRVEEG
                     RMGVVVTFLAIMELIKESLIEMVQSEPFGPIHVKSRGQDAVEVDH"
     gene            770..1393
                     /gene="scpB"
                     /locus_tag="PLW13_02420"
     CDS             770..1393
                     /gene="scpB"
                     /locus_tag="PLW13_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQY69250.1"
                     /translation="MQLKSIIEGALLAAGRPLTLDELADLFDLTDRPGAEELAAALQE
                     LCADCEGRGFEVRQVASGYRLQVRQELGPWISRLWEEKPARYSRALLETLAIIAYRQP
                     ITRGDIEEVRGVAVNPNIIRTLQERDWVRVVGQRDVPGRPELLATTRQFLDYFSLKGL
                     DDLPTLAEIKDFEQVNPEMTLAIEPPAEAADEAVATGDADDDEEPRQ"
     gene            1390..2481
                     /locus_tag="PLW13_02425"
     CDS             1390..2481
                     /locus_tag="PLW13_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY69251.1"
                     /translation="MNEGARLHKALADLGLGSRREIEGWIEAGRVTVNGRRAVLGQRI
                     ARGDRVAVDGKPVRLPDAEPAPIRVIVYNKPEGEICSRRDPEGRPTVFAALPRLRDGR
                     WIIVGRLDLNTTGLLLATDSGELANRLMHPGSSIEREYLVRVLGEVDAALLARLRAGV
                     PLDDGMAAFDGIRELEGEGSNRWFTVVLREGRNREVRRLWESQGCKVSRLKRVRFGTV
                     ALGPRERRGTCRELDETEVRALCASVGLIPPARAGAGERPGARRREAGAAAKRSPEPR
                     APAGRDQRGRGQGAAVRGEGRGRDAPAEAERPVRRNVDGSPANRAARRHDERAATPRP
                     ARRPEGAATAKRPPRRPEETAAKRPPRRR"
     gene            complement(2488..3384)
                     /locus_tag="PLW13_02430"
     CDS             complement(2488..3384)
                     /locus_tag="PLW13_02430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY69252.1"
                     /translation="MAGADILQFPHPIAQPAQPLAGAPAQRALGLAELELSNRLHRQL
                     DLRKLLELFLEELRLTLPVEGLCYSPPDSAECFVAGRGTTRTLRSRLHLAGEYLGQVE
                     AQVRGELHSPLAALLAPLAAPLSNALQHHRLKLLARKDPLTGLGNRMALDAALATEVA
                     RAQRFGQPFAMLIADIDHFKRINDSLGHSAGDQVIRAVATQARDCLRPYDQAFRFGGE
                     EFVVILSQTSLAKGMQIAERIRKRIAQRCSLDAATTGKITVSIGAAEIRDAETVDGLF
                     DRADRALYRAKSEGRNRSIAAD"
     gene            complement(3591..4346)
                     /locus_tag="PLW13_02435"
     CDS             complement(3591..4346)
                     /locus_tag="PLW13_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HQY69253.1"
                     /translation="MQPAHLYQAPPDLLAGRNILVTGAGDGIGRAATLAFAAHGATVV
                     LLGRTQAKLDAVYDEIVANGGAEPVIQLLDLKSAGDESYRALNEAIVDTLGCLHGVLH
                     NAALLGPRTPLEQYPTAKWLEVMAVNLSAPFALTKALMPALRLAPEASIVFSSSSVGR
                     RGRAYWGAYAVSKFGIEGLSQVLADELVNTSAIRVNCINPGATNTAMRREAYPAEAPA
                     SNPQPAQIMAAYLYLMGPASSGVTGCSLDAQGG"
     gene            complement(4361..5050)
                     /locus_tag="PLW13_02440"
     CDS             complement(4361..5050)
                     /locus_tag="PLW13_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY69254.1"
                     /translation="MERSARPRLRAVLFDLDGTLLDTAPDFVTAVNLLRAEHGREPLP
                     GEPIRRTVSHGARALVRLAFGIGEEDPAFEPLRQRLLALYEAHLLVDTVPFPGIAELL
                     QALAARGIRWGVVTNKPNYLAEPLLARARLEPPADVLVCPDHVSRTKPDPEPLLLACA
                     RLGCAVTEAVYVGDHARDIQSGRNAGMRTIAATYGYLEPDEAVTQWQADYHADSVPAI
                     AHILFTHFLFT"
     gene            complement(5050..5763)
                     /gene="ubiG"
                     /locus_tag="PLW13_02445"
     CDS             complement(5050..5763)
                     /gene="ubiG"
                     /locus_tag="PLW13_02445"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQY69255.1"
                     /translation="MKTDTTPNVDAAEIAKFEALASRWWDRAGDFRPLHQMNPLRANY
                     IDARSPVAGRRVLDVGCGGGILSEALAQRGATVTGIDMGEAPLAVARLHLLESGLEID
                     YRHANAEALAEECPGGFDIVTCLEVLEHVPDPGSLVAACARLVRPGGDVYFATINRNP
                     KSYAFAILGAEHILKLLPKGTHDYARFIRPSELAAWTRQAGLQTEDISGMLYNPLTQA
                     FSIGRDVDVNYLVHARKPG"
     gene            complement(5802..7118)
                     /locus_tag="PLW13_02450"
     CDS             complement(5802..7118)
                     /locus_tag="PLW13_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HQY69256.1"
                     /translation="MENVDQIIRARWILPVNPRDTVLEHHAVALRDGRIVAIVPGDCL
                     AGAFHAPLETALDDHLLMPGLVNTHGHAAMTLLRGLADDHTLMDWLQDHIWPAEARWM
                     GEDFVRTGTRLAIAEMLASGTTCASDMYFFPDVAAAAVVEAGFRAQIAFPILEHASAW
                     GANADEYLARGLQVRDDYRSCPRLSFAFGPHAPYTTSDQTLARVAMLAAELDSPVQMH
                     VHETAGEVHEAIAHHGCRPLARLEGLGLLSPRFQAVHMTQVAPEDLELLVRNQVQVLH
                     CPSSNLKLAAGFCPVRTLGAAGINVALGTDGAASNNALDLFAEMRLAALLAKGSSGDP
                     TALSAHAAVRMATLGGAEALGLAERIGSLEPGKDADMIAVDMRRIACAPVYDAHSALV
                     YTGSGAAVTHAWVEGRAVLSGRRLCTLAPDELHAEALAWQHRIAGR"
     gene            7333..9927
                     /gene="gyrA"
                     /locus_tag="PLW13_02455"
     CDS             7333..9927
                     /gene="gyrA"
                     /locus_tag="PLW13_02455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017337295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQY69257.1"
                     /translation="MGELAKEIVPVSIEGELRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQPFSMRYVL
                     VDGQGNFGSVDGDSAAAMRYTEIRMRKIAHELIADLDKETVDFVPNYDGTEMIPAVFP
                     TRIPNLLVNGSSGIAVGMATNIPPHNLGEVVRACLALIDNPELEIDDLIEIIPGPDFP
                     TGGIINGRQGIVEAYRTGRGRIYVRARAEVITDDSSGRDTIIITEIPYQLNKARLIEK
                     IAELVKEKRIEGISELRDESDKDGLRVVIELRRGESGEVVLNNLYAQTQLESVFGINV
                     VALVDGQPRTLNLKELLEAFLRHRREVVTRRTVYLLRKARERGHVLEGLAIALANIDA
                     VIELIRNSSNPGEARERLVARAWEPGSVLVMLDKAGPDACRPEDLPREFGLREGGYHL
                     SPEQAQAILDLRLHRLTGLEHEKLLEEYQQKLAEIADYLEILGDPGRLMRVIREELEK
                     VVADYGDKRLTEIVSSRLDLTREDLIPQEERVVTVSFSGYAKSQPLDAYQSQRRGGVG
                     RSGAPVREEDSIEHLLVANSHHTILCFSDRGRVFWLKVYEIPVASRGAKGRPLVNLLK
                     LEGDERITSLLPVGSFDAERFVFMATSDGTVKKTPLDAFSRPRGNGLIAIDLGEDDHL
                     VGTAITDGNSDVLLFSSGGKAVRFRESEVRAMGRNAHGVRGMRLPAGQRVVSLIVPDD
                     SASHVLTLSANGYGKRTVTAEFPRHSRGGQGVIAMQASERNGALVGAIRVLPSDEMML
                     ISDQGTLLRTVVDQISLLSRNTQGVKVMNVRDGETLVGMARIAEAEGPVAAHEDEDGA
                     SAADPADG"
     gene            9969..11051
                     /gene="serC"
                     /locus_tag="PLW13_02460"
     CDS             9969..11051
                     /gene="serC"
                     /locus_tag="PLW13_02460"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQY69258.1"
                     /translation="MTRVFNFCAGPAALPEAVLARARDELLDWHGRGLSVMEMSHRGK
                     DMVAIAEKAEADLRELLGISDDYAVLFLQGGATAQFAAVPLNLLGAAGKADYVNTGQW
                     SVKAIAEAQKYCEVNVAASSEATRFTGVPAQSVWQRSPAAAYLHYTPNETIGGVEYGW
                     VPEVGATPLVADMSSTILSRPIDVNRFGVIYAGAQKNIGPAGLVLVIVRRDLLGRPDP
                     RTPAVLDWTLQAKNASMYNTPPTYSIYLAGLVFQWLKDQGGLAAMGEVNRRKAEALYG
                     YIDGSGFYANPVEKASRSWMNVPFTVADAALEKPFVAEAEKAGLLYLEGHRSVGGMRA
                     SIYNAVGMDAVTALIDFMKDFAARKG"
     gene            11068..12186
                     /gene="pheA"
                     /locus_tag="PLW13_02465"
     CDS             11068..12186
                     /gene="pheA"
                     /locus_tag="PLW13_02465"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQY69259.1"
                     /translation="MTDSASALDRIRAEIDGIDLQLQALLNRRAACAQQVAEVKKAEL
                     LAAHGHVEPGQVNFYRPEREAQVLRRVMARNDGPLPATEVAHIFREIMSACLALEKPM
                     TIAFLGPEGTFTQAAAVKHFGYAVLSRPLATIGAVFEEVESGESDYGVVPVENSTEGM
                     VTHTLDNFVRSTLKICGEVELPIRHHLLGAPGCRLENVTRICAHQQALAQCRAWLEKN
                     WPRIEQQATSSNAEAARLAAADATVAAIAGDMARETYGLVTLAAGIQDYADNTTRFLV
                     VGREMVKPSGSDKTSIIVSTHNKPGALFKLLEPFQREQLMLTRIDTRPSRSQPWTYLF
                     FIEFEGHQDDPKVAAVLAELEEQTVMLKILGSYPQAVI"
     gene            12191..13081
                     /locus_tag="PLW13_02470"
     CDS             12191..13081
                     /locus_tag="PLW13_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQY69260.1"
                     /translation="MSARVGTLLFVGLGLIGGSLAAALRGTGFAARMTAFNRNRATLD
                     FALGAGLIDAAPASLEEAIAEADLIVVGVPTLSVGRIFAAIRAHARPDVLITDVASVK
                     GSVVAAAREAFGAVPPRLIPGHPIAGAEKSGVEAARADLFVRHRVILTPLPESDPEAL
                     ATIDAMWRLTGAEVVRMDVAAHDRVLAMTSHLPHLLAFALVDSLARQPASEEIFRCAA
                     GGFRDFTRIASSDVVMWRDIALANRDALLGALDGFTHTLDAVRAAVASGDGEELEQVF
                     GRARAARERYLRWLERGESR"
     gene            13110..14438
                     /gene="aroA"
                     /locus_tag="PLW13_02475"
     CDS             13110..14438
                     /gene="aroA"
                     /locus_tag="PLW13_02475"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQY69261.1"
                     /translation="MTHDGRIDYIASGGAVLRGELRVPGDKSISHRSIMLGSLAEGTT
                     EVSGFLEGEDSLATLAAFRAMGVRIEGPEQGRVRIEGVGMHGLRAPAAALYLGNSGTS
                     MRLLAGLLSAQRFDVCLSGDESLTRRPMARVADPLRQMGAQIETAANGRPPLWLRGGH
                     ALHGIEYHMPVASAQVKSAVLLAGLYAAGTTRVIEPAPTRDHTERMLSGFGYPVLRDG
                     ARVELVGGQSLRATRVDVPADISSAAFFLVAAAITPGSELLLKNVGVNPTRTGVINIL
                     RAMGASIELLDPRESGGEPVADLRVRAAPLRGIEIPLEQVPLAIDEFPALFIAAACAE
                     GETVLRGAEELRVKESDRIDAMARGFEALGVRHETLPDGIRIRGGAIGGGTIDSRGDH
                     RIAMAFAVASLRASAPIEILDCANVATSFPGFRELAAAAGLTVTHKATGA"
     gene            14469..15161
                     /gene="cmk"
                     /locus_tag="PLW13_02480"
     CDS             14469..15161
                     /gene="cmk"
                     /locus_tag="PLW13_02480"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQY69262.1"
                     /translation="MITEEDIPVIAVDGPSGSGKGTVCRLLARELGWNLLDSGAMYRL
                     VGLAARNHGVRLDNHAALDVLAGHLDVQFRPGEDTAEARVILEGEDVSQLLRTEEVGA
                     LASEVAAVPAVRASLLRRQRAFKIRPGLVADGRDMGTVVFPEAQFKVFLTASADERAR
                     RRYKQLKDKGEAVSLPRLVADIEARDRRDTERSVAPLRAAPDAVTVDTTGMGIQEVFD
                     LLMAEVKERGML"
     gene            15325..16998
                     /gene="rpsA"
                     /locus_tag="PLW13_02485"
     CDS             15325..16998
                     /gene="rpsA"
                     /locus_tag="PLW13_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQY69263.1"
                     /translation="MTENFAQLFEESLKTVEMDVGSIITGVVIDIDNEWVTVHAGLKS
                     EGVIPRVQFLDDNGEFHLEIGDEVYVALEAIEDGNGETRLSREKARRAETWKALEGSF
                     ENSEVVRGAINGKVKGGFTVDIQGIRAFLPGSLVDVRPVRDTAHLEGQTLDFKVIKLD
                     QKRNNIVVSRRAVLEQANSAERESLLANLQEGATLKGVVKNLTDYGAFVDLGGVDGLL
                     HITDMAWRRIKHPSEVVKVGDEIDVKVLKFDRETNRVSLGLKQLGSDPWVNIKARYPE
                     NARVKARVTNLTDYGCFAELEQGVEGLVHVSEMDWTNKNIHPSKVVQVGDEVDVMILD
                     IDEERRRISLGMKQCSENPWDAFASTHTKGERISGKIKSITDFGLFIGLDGNIDGLVH
                     LSDISWHETGEEAVRAYKKGDDVNTVILSIDPERERISLGIKQLEDDPFNNFVAINDK
                     GSIVTGKVVDVDAKGAVIELAEDVQGTLKASEISRDKIEDARNVLKIGDSVEAKIINV
                     DRKNRVIGLSVKSKDVQDEKEAIKALKEESEAAPATTIGDLIKAQMERK"
     gene            17144..17446
                     /gene="ihfB"
                     /locus_tag="PLW13_02490"
     CDS             17144..17446
                     /gene="ihfB"
                     /locus_tag="PLW13_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017337302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HQY69264.1"
                     /translation="MTKSELIERIATQQKQLSPKDVELAVKMIIEYMAQSLSTGDRIE
                     IRGFGSFSLHYREPRRGRNPKTGDAVDLLGKYVPYFKPGKEMRERVNLNADQEISG"
     gene            17457..17756
                     /locus_tag="PLW13_02495"
     CDS             17457..17756
                     /locus_tag="PLW13_02495"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQY69265.1"
                     /translation="MKRLKRLLLLALVLGVGLWAFLFSLANSEPVTLDLVFARLEPAA
                     LSVWLIAAFVGGGLCGLFAGSVAIWRAQRALPRSRRVPAAPAVVAPPAGTTAATR"
     gene            17822..18565
                     /gene="pyrF"
                     /locus_tag="PLW13_02500"
     CDS             17822..18565
                     /gene="pyrF"
                     /locus_tag="PLW13_02500"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQY69266.1"
                     /translation="MQSPSGSPDRCADAGPRIVVALDYATAAAALAMAGRLDPRRCRL
                     KVGKELFTAAGPELVRSLVARGFEVFLDLKFHDIPNTVAGAVRAAAELGVWMVNVHAG
                     GGRRMMQGARAALASLPGPRPLLIAVTVLTSMEADELAETGVSGGLEQQVLRLAGLAL
                     ECGLDGVVCSAREAPLLRSHFGAAPVLVTPGIRPASAAPDDQRRTLTPAEALAAGSDY
                     LVVGRPITAAADPGAALEAILSDISYRFS"
     gene            18639..18953
                     /locus_tag="PLW13_02505"
     CDS             18639..18953
                     /locus_tag="PLW13_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQY69267.1"
                     /translation="MKLADVLKASVLGLSLLVLPAAHAAAEQASAGGAPVQQASQQVN
                     INTADVATLAGSLRGVGIKKAEAIVAYRSEHGPFKSVDELVNVKGIGDKMLEQLRSQV
                     SL"
     gene            19050..19991
                     /locus_tag="PLW13_02510"
     CDS             19050..19991
                     /locus_tag="PLW13_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY69268.1"
                     /translation="MSRIALVTGGGGYIGSALCRALQGRGWRVLATARDPAIVTATPC
                     IALELEDAAAGHVDPQVCRDAEVVFHLAGIAHRGADALALERINCDATLALARAAEAA
                     GVAHFVYLSSLYAGACDPGAPGARGDAYARSKRLAELGLLGAEPNWRMRVTIVRPALV
                     YGGAMKGRLATLRALALKGWLPDLPPVGNTPMIALPDLVHALALLGAHPSISGNMFTV
                     SDGERYPLDRIAHAMHRLAGRGSGAWRIPAGPFALALAAAARLGRLPVLGSRLALPRG
                     LADWTVDPDQRLRALGWAPALTLETALAPAMPDGERA"
     gene            20005..21039
                     /locus_tag="PLW13_02515"
     CDS             20005..21039
                     /locus_tag="PLW13_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQY69269.1"
                     /translation="MRALAVVATAFTVGWCVTWCYCRWALRAGHLDVPGARSSHALAT
                     PTGGGAGIVAGCAAALVATMAGGAVHADWLLVLCLALLMAALGYADDRRALPVLPRLL
                     GQFGAAAAVMACALAVAGIGPGGAGLLLLPPGLLALVWLNNAFNFMDGIDGLAASQAV
                     FMAGAAAWLVAFGAGADDSVLLLGGIAGAASGFLALNWPPARIFMGDTGSQPLGLLLG
                     GAAAVTVARGELSLAVWAILWAAFLADATLTLLHRAWRRQPLLQAHRDHAYQRLARRR
                     GAHRPVTLGCIALNLCWLLPLAWLAHRMPRLGLVCVLIAYVPVCLGVLAVLLRDGRES
                     PAASSGRPVK"
     gene            21150..23159
                     /locus_tag="PLW13_02520"
     CDS             21150..23159
                     /locus_tag="PLW13_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQY69270.1"
                     /translation="MNLLFRMLREAARASSAFITLLYERFVVLPHNVKQALLLLVDLV
                     SIPVALFIATTLRYGTLQVEIGSSELLVCAATVLVSAVVFLRLGLYRAIVRFMGHEAI
                     VAIVKGVTISALLLALFTLLTRAEVPRSLPMIYWCLMLICVGGSRLVLRSWYRHRRER
                     SARRIAIYGAGSAGRQLLTALLHGEQFDPVLFIDDNPALRGRVINDVPVIGLDQLPAK
                     IRHSGIAEIFLAMASIGADRRREITQALTELAVHVRTIPRFEDLVRGRADISEVQEIT
                     LEDLLGREPVPPRPDLLAVCIAGKRVMVTGAGGSIGSELCRQILAESPAELLLVDASE
                     FALYNIERELLDVAARGGLATRIVALLGSVQDHARMRLLLEGFQVQTVYHAAAYKHVP
                     LVEHNVIEGVRNNVMGTLTVARAAAEAKVRTFVLVSTDKAVRPTNVMGASKRLAEMVM
                     QSLAAANAGGTRYVIVRFGNVIGSSGSVVPLFQEQIAAGGPVTVTHAEAERYFMTIPE
                     AAQLVLQASAMGSGGDVFVLDMGARVRIHDLARRMIHLAGKEVRDEANPGGDIEIRIT
                     GLRPGEKLREELLSEGSVGATGHPMILRAVEECLPWAELERVLEELSEACVDYDCKRV
                     RDIFARVVRGFDDRYPCADALGERLNERAAGQRPRLKMLAPVKSG"
     gene            23230..24204
                     /locus_tag="PLW13_02525"
     CDS             23230..24204
                     /locus_tag="PLW13_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQY69271.1"
                     /translation="MRYVDVFNGDADGICALLQLQLSSPRDSELVTGVKRDIDLLRQV
                     RAGAGDRITVLDVAMEKNRADLDRLLAAGASVFYVDHHIPGEIPEHAGLQAIINTAPE
                     VCTSLLVNGHLKGAHARWAVVGAFGDNLGESARRLATQIGLDGEALAAAENLGNYINY
                     NGYGPSIEDLHFAPAELFRRLRPYADPLAFIAGDRETFGRLEEGFHGDMGMAAALKPM
                     HESASAAVFLLPDEPWARRVSGVYSNDLVNLHPARAHAVLTEIGGGDLLVSVRAPLAN
                     RTGADEVCRQFATGGGRKAAAGINRLAREDLARFIDVFCSFYAAHAGS"
     gene            24267..25274
                     /locus_tag="PLW13_02530"
     CDS             24267..25274
                     /locus_tag="PLW13_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69272.1"
                     /translation="MLRLLMLLWCVAPEATRAEGEIQVGAPRCQPSQGCETAEAQAPA
                     QHWVDTSYAYVTSRTDSLAVWLDSFFGTVTSDMESADSVLRLRTEYEWNEEDGEDYKV
                     RLRGKVDLPRLDERLSLVFSEEGEEREEVIPNSAGNDDDVGLQFRVRERSRSRLWFTV
                     GTNSSLDFKSSVRYRYIQPIGDDWRVQYTQRFHFKEGDGFGTTTRGDLDYLISRDRIV
                     RWTNQVEYGEETNGAEWGTRLSYQLRLSEKDALSYFGAVAGETDPENLTQSYALGVRY
                     RRNFLRPWIFFEIEPSYAWRRESVEESRSSLWLLTLRLEFLEERYNRRGHDEANRQTV
                     D"
     gene            25359..26666
                     /locus_tag="PLW13_02535"
     CDS             25359..26666
                     /locus_tag="PLW13_02535"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQY69273.1"
                     /translation="MSQLHAVPTAVTDLEIGMFVTALDRPWIETPFLVEGFYVTSQAD
                     IDEVEKHCKFVYVDVYRCRLGAGRRNSRVVGAAGQKGATIPLPQEPALETIKFRQRSA
                     TAIAEMFPHRKLKPFEDSSTFGGELAVARPVYAEVLRAYRQMMEAYRAGGRLDVSGVR
                     EVVNPMVESVIRNPDACVWLACSKDEQSYTWGHSAGASVWAVALGRQLGLPRVDLQRL
                     AMGALLLDVGKLKVPEALLLKRERLTPGEFQLIKSHVEFGLQMLEGTGILSRSVTDML
                     EFHHERHGGHGYPRGIAGNDIPVFGRIAAIVDCYDAITSRRPYAPPMSPSHAVKQLYA
                     WRDIDFDAELVEEFIQAIGIYPAGTLVELSNGEVGVATAGYRTRRLRPQLLLVLDRDK
                     KPYASPRPLDLLAVTHDQDGKPLEIATSLEPGSFGVDPVTLVV"
     gene            26958..28247
                     /locus_tag="PLW13_02540"
     CDS             26958..28247
                     /locus_tag="PLW13_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HQY69274.1"
                     /translation="MRLSKAVVCGGLILAGATQANAGSVDAKLLDMLKANGSITAAQH
                     AELSADLVRETREEKRAAKEVVKEKDFVAFRQAAGWAESTKLTGDMRVRQETIDVDGE
                     PDFTGSRNKDRQRIRARLGAFTKINPEVETGIQVASGNGADRRSTNENMDNYFDKKAV
                     WLDLAYINYNPVAVPGLKTFAGKMKQPWMSMGDVIWDGDINPEGVAAGYTKKNGTTTF
                     FGSGGYYVLKDAVDSEGFEIENDLALYQLQGGVAFDVSDSARWTLGASVYDFNNDKYG
                     STSSFRANGNTTDKFGLGEIFTQLDVIGLPLPLSLYGQYVQNYEARDMAATTIDDSDE
                     DTAYLFGLRTNIAGVAFDYNYRDVDANGVVGGFTDSDFAVGYTNSSGHKLKLKYDFMK
                     NFSIGATYFMAESDAVSSRKLKGVDADTLHVDLEAKF"
     gene            28326..29330
                     /locus_tag="PLW13_02545"
     CDS             28326..29330
                     /locus_tag="PLW13_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY69275.1"
                     /translation="MKKISGSLKAAIGVAALCGGFLSQQAGAASVIEIDGSSTVYPIT
                     EAVAEEYQAAGGARVTVGISGTGGGFKRFCRGETDISDASRPILKEEMVLCAQAGIKY
                     FELPVAYDAITVVVSKDNDFVDHLTVEELKKMWEPAAAGKITSWKQVRDSFPDVPLKL
                     AGAGADSGTFDYFTEAVVGVAKSSRGDYQASEDDNVTVQFVSRDKGALGYFGLAYYEE
                     NKDTLRAVPIVAPGGSTGVVPSIATVNDGTYTPLSRPIFIYVKEKSVQRPEVRKFVEF
                     YLKEGGALTREVGFVDLPAAAYELALKNFNEGKLGSGFGGKSEVGLKVEELLAREGKL
                     "
     gene            29434..30378
                     /gene="pstC"
                     /locus_tag="PLW13_02550"
     CDS             29434..30378
                     /gene="pstC"
                     /locus_tag="PLW13_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQY69276.1"
                     /translation="MTPNSAKPAAAETYPGNRFAHRPLRHVSERLIEGVLFLAAFSAV
                     AVTVAIVFILVAESAAFFEHVPLIDFLTDTQWTIMFSEKHYGVAPLLAGTLVTTTVGL
                     LLAVPVGTITAIYLSEFAGPRTREIVKPVLELLAGIPTVVYGYFALVMVTPALQYFMP
                     DLPGFNMLGPGLVIGIMIIPYISSVAEDAMRAVPLHMREGSYAMGATRLQTALRVVVP
                     AATSGIVAAYILGISRAIGETMVVAVAAGQQPNFTWDPREPGATITAFIVQVALGDLP
                     HGSIEYQSIFAVGLVLVLMTLFFNVLGHLARKRFREAY"
     gene            30378..>30948
                     /gene="pstA"
                     /locus_tag="PLW13_02555"
     CDS             30378..>30948
                     /gene="pstA"
                     /locus_tag="PLW13_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQY69277.1"
                     /translation="MTQDMQQIRRLVARGKQWDKVFVIIGLICLMIGVLTLAVLLAGL
                     VQDGAGRLNWDFFTSFPSRRAENAGILSAIVGSSLVMLLTAITAIPLGVAAGVYLEEY
                     ARRNWVTEIIEINVNNLAGVPSIIYGLLALGLFVYALDFGQSILSAGLTLALLILPII
                     IVATREAIRSIPSSIREGAYALGATRWQMV"
BASE COUNT         4812 a        10278 c        11043 g         4815 t
ORIGIN      
        1 gtggccgtgg cccgcctgta cggcgagccg ctgttcgcca tgccacagga cctgtacatc
       61 ccgccggatg cgctggaagt gttcctcgag gccttcgagg gcccgctgga cctgctgctc
      121 tacctgatcc ggcgccagaa cctcgacatc ctcgagatca acgtcgcgca gatcaccgag
      181 cagtacatgc agtacgtcga gctgatggac gcgatgcagt tcgagctcgc cgccgaatac
      241 ctggtgatgt cggcgatgct ggccgagatc aagtcgcgca tgctgctgcc gcgccagcag
      301 agcgccgccg aggaggagac cgacccgcgc gccgacctga tccggcgcct gcaggagtac
      361 gagcagttca agcaggcggc cgaggatttg gacgggctgc cgcgcctgga gcgcgacgtg
      421 ttccaggccg ctgccgagcc gccggaactg cgccgcagca agccgcagcc ccaggtggag
      481 ctgcgcgagt tgctgctcgc cttcggcgag gtggcgcggc gcgcctcgat gaacgtcagc
      541 caccacgtgc agatggagcg gctgtcgacg cgcgaacgca tggtcgagat cctcgcgcgg
      601 ctgcatacgc cgcatttcgt gcccttcgtc agcctgttcc gcgtcgagga agggcgcatg
      661 ggcgtggtgg tgaccttcct cgccatcatg gagctgatca aggaatcgct gatcgagatg
      721 gtgcagtcgg agccgttcgg acccattcac gtcaaatccc gggggcagga tgcagttgaa
      781 gtcgatcatt gagggcgcgc tgctcgcggc cggccgtccg ctcacgctgg acgagctcgc
      841 cgacctgttc gacctcaccg accgccccgg cgccgaggag ctggccgcgg cgctgcagga
      901 gctgtgcgcc gactgcgagg gccgcggatt cgaggtgcgc caggtcgcca gcggctaccg
      961 cctgcaggtg cgccaggagc tggggccgtg gatctcgcgg ctgtgggaag agaagccggc
     1021 acgctactcg cgcgcgctgc tcgagaccct ggcgatcatc gcctaccgcc agccgatcac
     1081 gcgcggcgac atcgaggagg tgcgcggcgt ggcggtcaat cccaacatca tccgcaccct
     1141 gcaggagcgc gactgggtgc gcgtcgtggg acagcgcgac gtgccggggc ggcccgagct
     1201 tctcgccact acgcgccagt tcctcgacta tttcagcctg aaggggctgg acgacctgcc
     1261 gacgctggca gagatcaagg atttcgagca ggtgaacccg gaaatgacgc tcgcgatcga
     1321 gccgccggcc gaggctgctg acgaggccgt cgctaccgga gatgccgatg acgacgagga
     1381 gccgcgccaa tgaatgaggg cgcacgactg cacaaggcgc tggcggatct cggcctgggt
     1441 tcgcggcgcg agatcgaggg ctggatcgag gccggccgcg tcacggtgaa cggacggcgc
     1501 gcggtgctgg gccagcggat cgcgcgcggc gaccgggtcg ccgtcgacgg caagccggtg
     1561 cgcctgcccg acgcggagcc cgcgccgata cgcgtcatcg tctacaacaa gcccgagggc
     1621 gagatctgca gccgccgcga cccggaaggc cgcccgaccg tctttgccgc gttgccgcgc
     1681 ctgcgcgacg ggcgctggat catcgtgggc cgcctcgacc tcaacaccac gggcctgctg
     1741 ctcgccactg attcgggcga gctggcgaac cgcctcatgc atccgggctc gtccatcgag
     1801 cgcgagtacc tggtgcgggt gctgggtgag gtggatgccg cgctgctggc gcgcctgcgt
     1861 gccggcgtgc cgctcgacga cggcatggcg gccttcgacg gcatccgcga gctcgagggc
     1921 gagggcagca accgctggtt cacggtggtg ctgcgcgagg ggcgcaatcg cgaggtgcgc
     1981 cggctgtggg agtcgcaggg ctgcaaggtc agccgcctta aacgcgtgcg tttcggcacg
     2041 gtggcgctgg gtccacgcga gcgccgcggc acctgccgcg aactggacga aaccgaggtg
     2101 cgcgcgctgt gcgcctcggt ggggctgatc ccgccggcgc gcgccggtgc cggcgagcgc
     2161 ccgggcgcgc gtcggcgcga ggcgggcgct gcggcgaagc gttcgcccga gccacgcgcg
     2221 ccggccggac gcgatcagcg cggacgcggg cagggcgcgg cagtgagagg cgaggggcgg
     2281 ggtcgtgacg cgcctgccga ggcggagcgt cccgtgcgtc gcaacgtcga cggctcgccg
     2341 gccaatcgcg ctgcgcgccg ccatgacgag cgcgccgcga cgccacgtcc ggcgcgccgg
     2401 ccggaagggg ccgccacggc caaacgtccc ccgcgccggc ccgaagagac cgccgccaag
     2461 cgtcccccgc gccggcgtta ggaatcctca gtcggcggcg atgctgcggt tgcgcccttc
     2521 gctcttggcg cggtagagcg cgcgatcggc gcgatcgaac aggccgtcga cggtttcggc
     2581 gtcgcggatc tcggcggcgc cgatgctgac cgtgatcttg cccgtggtcg cggcgtccag
     2641 gctgcagcgc tgcgcgatgc gcttgcggat gcgctcggcg atctgcatgc ccttggcgag
     2701 cgaggtctgg ctcaggatca ccacgaactc ctcgccgccg aaacggaagg cctggtcgta
     2761 ggggcgcagg caatcgcgcg cctgtgtcgc cacggcgcgg atcacctggt cgccggcgct
     2821 gtggccgaga gagtcgttga tgcgcttgaa atggtcgatg tcggcaatca gcatcgcgaa
     2881 cggctgcccg aagcgctgcg cacgcgccac ctcggtggcc agcgcggcgt cgagcgccat
     2941 gcggttgccg aggccggtca gcggatcctt gcgcgccagc agcttcaggc gatggtgctg
     3001 cagcgcgttg ctgagcggtg cggccagcgg cgcgagcaac gcggccagcg gcgagtgcag
     3061 ctcgccgcgc acctgggcct cgacctggcc gaggtattcg ccggccaggt gcagccgcga
     3121 acgcaatgtc ctggtggtcc cgcgcccggc gacgaagcac tcggccgagt cgggcggcga
     3181 atagcacagg ccctcgaccg gcagcgtgag gcgcagctcc tcgaggaaga gctccagcag
     3241 cttgcgcagg tccagctgcc ggtgcaggcg gttgctcagc tcgagttccg ccaggcccag
     3301 cgcacgctgg gccggcgcgc ccgccagcgg ttgtgccggc tgcgcgatcg ggtgcgggaa
     3361 ctgcaggatg tctgctccgg ccattgtcgt cgtccgcctg ttgctggggt cgcaggagcg
     3421 acgaagcgat attcatgcca ctggctgaag tgtcgcgcgc aacagacgga aaacattgat
     3481 aaaagttcgg ctgccgcggc atttccccgc gccggacagc ggagaggccc gacaattaat
     3541 tgacgcagat cccggtagct ggctgccgcg gtcgccgcgg tggcaggctc tcagccgccc
     3601 tgcgcgtcca gggaacagcc ggtgacgccg ctgctggccg gccccatcag gtagagatag
     3661 gcggccatga tctgcgccgg ctgcgggttg ctcgccgggg cctcggccgg ataagcctcg
     3721 cgccgcatcg cggtgttggt cgcgcccggg ttgatgcagt tgacgcggat cgccgaggtg
     3781 ttgaccagtt cgtcggcgag tacctggctc aggccctcga tgccgaactt cgacaccgcg
     3841 taggcgcccc agtacgcgcg cccgcgccgc ccgacgctgg agctggagaa cacgatcgag
     3901 gcctcggggg ccaggcgcag cgccggcatc aatgccttgg tgagcgcaaa cggcgcgctc
     3961 aggttgaccg ccatcacctc gagccatttc gcggtcgggt actgctccag cggcgtgcgc
     4021 ggtccgagca gcgcggcgtt gtgcagtacc ccgtgcaggc agcccagggt atccacgata
     4081 gcctcgttca gggcgcggta actctcgtcg ccggcgctct tcaggtcgag cagctggatc
     4141 acgggctccg cgccgccgtt ggcaacgatt tcgtcgtaca ccgcgtcgag cttggcctgc
     4201 gtgcgcccga gcagcaccac ggtggcgccg tgggcggcga aggcgagcgt ggcggcacgg
     4261 ccgatgccgt cgccggcccc ggtcacgagg atgttgcgtc cggccagcag atcgggcggt
     4321 gcctggtaga gatgggcggg ctgcatgcgg gtttgcgctc tcaggtgaac aggaaatggg
     4381 tgaacaggat gtgcgcgatc gccggcaccg agtccgcgtg gtagtccgcc tgccactgcg
     4441 tgacggcttc gtcgggctcc aggtaaccgt aggtggcggc gatggtgcgc atgcccgcat
     4501 tgcgcccgga ctggatgtcg cgggcgtggt cgccgacgta gaccgcctcc gtcaccgcgc
     4561 agcccaggcg cgcgcaggcg agcagcagcg gctcggggtc cggcttggtg cggctcacgt
     4621 gatccgggca gaccagcacg tcggccggcg gctcgaggcg ggcgcgcgcg agcagcggtt
     4681 cggcgaggta gttcggcttg ttggtgacca cgccccagcg aatcccgcgc gcggcgagcg
     4741 cctgcagcag ttcggcgatg ccgggaaacg gcacggtatc gaccagcagg tgcgcctcgt
     4801 agagcgcgag caggcgctgg cgcagcggct cgaaggccgg gtcctcctcg ccgatgccga
     4861 aagcgaggcg caccagggcg cgcgcgccgt gcgagacggt gcggcggatc ggctcgccgg
     4921 gcagcggctc gcggccgtgc tcggcgcgca gcaggttcac ggcggtcacg aaatccgggg
     4981 cggtgtcgag cagggtgccg tcgaggtcga acagcaccgc gcgcaggcgg gggcgggcgg
     5041 agcgctccat cagccgggct tgcgcgcgtg cacgaggtag ttcacgtcga cgtcgcgccc
     5101 gatgctgaac gcctgcgtga gcgggttgta gagcatgccc gagatgtcct cggtctgcag
     5161 cccggcctgg cgcgtccagg ccgcgagctc cgaggggcgg atgaagcgcg cgtagtcgtg
     5221 cgtgcccttg ggcagcagct tcaatatgtg ctcggcgccg aggatcgcga aggcgtagct
     5281 cttcgggttg cgattgatgg tcgcgaaata gacgtcgccg ccggggcgca ccagccgcgc
     5341 gcaggcggcg accagcgagc cggggtcggg cacgtgctcg agcacctcga ggcaggtcac
     5401 gatgtcgaag cctcccgggc attcctcggc cagcgcctcg gcgttggcgt ggcggtagtc
     5461 gatctccagg cccgattcca gcaggtgcag ccgcgccaca gcgagcggcg cctcgcccat
     5521 gtcgatgccg gtgaccgtgg cgccgcgctg cgccagcgcc tcgctgagga tgccgccgcc
     5581 gcagcccacg tcgagcacgc gccggcccgc gaccggggag cgcgcatcga tgtagttcgc
     5641 gcgcagcggg ttcatctggt gcagcggacg gaagtcgccg gcgcgatccc accagcggct
     5701 cgccagcgcc tcgaacttgg cgatttccgc cgcatcgacg ttgggtgtcg tatctgtctt
     5761 cattcaactg gacctgtgtt gcgattgagc gggccgcgag ttcagcggcc agcgatgcgg
     5821 tgctgccagg cgagcgcctc ggcgtgcagt tcgtcgggtg ccagcgtgca cagccggcgt
     5881 ccggacagca cggcgcggcc ctcgacccag gcgtgggtga cggcggcgcc gctgccggtg
     5941 tagacgagcg ccgaatgcgc gtcatatacg ggcgcgcagg cgatccggcg catgtcgacg
     6001 gcgatcatgt cggcgtcctt gccgggctcg aggctgccga tgcgctcggc caggccgagt
     6061 gcctcggcgc cacccagcgt ggccatgcgc acggccgcgt gcgccgacag cgccgtcggg
     6121 tcgccgctgc tgcccttggc cagcagtgcc gccaggcgca tctcggcgaa caggtcgagc
     6181 gcgttgttgc tggcggcccc gtccgtgccg agcgcgacat tgatgccggc ggcgcccagc
     6241 gtgcgcaccg ggcagaagcc cgcggcgagc ttcaggttcg agctggggca gtgcagcacc
     6301 tggacctggt tgcgcaccag cagttcgagg tcctcggggg cgacctgggt catgtgcacg
     6361 gcctggaaac gcggcgacag caggccgagt ccctcgaggc gcgccagcgg gcggcagccg
     6421 tggtgcgcga tcgcctcgtg cacttccccg gccgtctcgt gaacgtgcat ctgcaccggg
     6481 ctgtcgagct ccgcggcgag catcgccacg cgcgccaggg tctggtcgct ggtggtgtag
     6541 ggcgcatggg ggccgaaagc gaaggagagc cgcgggcagc tgcgatagtc gtcgcgcacc
     6601 tgcagccccc tggccaggta ctcgtcggcg ttcgcgcccc aggcgctggc gtgctcgagg
     6661 atcgggaagg cgatctgggc acggaaaccc gcttccacca cggccgcggc ggccacgtcg
     6721 gggaagaaat acatgtcgct ggcgcaggtg gtgccgctgg cgagcatctc ggcgatcgcg
     6781 agccgggtgc cggtgcgcac gaagtcctcg cccatccagc gcgcctcggc cggccagata
     6841 tggtcctgca gccagtccat cagcgtgtgg tcgtcggcga gcccgcgcag cagcgtcatc
     6901 gccgcgtggc cgtgggtgtt caccaggccg ggcatcagca ggtggtcgtc cagggcggtc
     6961 tcgagcgggg catggaatgc cccggcgagg caatcgccgg gcacgatggc cacgatgcgc
     7021 ccgtcgcgca gcgcgacggc atggtgctcc agcacggtgt cgcgcgggtt caccggcagg
     7081 atccagcgcg cgcggatgat ctggtcgacg ttctccaata cccctcccgg gtggttcggg
     7141 tgcccaaggc ggcggcaagt ataaccgcaa gccctgcgcc ccggaggcgt gcgcggcggg
     7201 ggtgacgccg gttagccccg cggcgggggg ctgtgctatg atgccgcccc tttgaagccg
     7261 ccgaaatccg cgcgcgacag cctgctgccg cagctccggt attggccgaa tccatagact
     7321 tttcaggttg ttatgggcga attagcaaaa gaaatcgtac cggtcagcat tgaaggtgag
     7381 ttgcgccagt cctacctcga ttacgccatg agcgtgatcg tggggcgggc gctgccggac
     7441 gtgcgcgacg gcctgaagcc ggtgcaccgg cgcgtgctgt tcgcgatgag cgaactgggc
     7501 aacgactgga acaaggccta caagaagtcg gcccgcatcg tgggcgacgt gatcggcaag
     7561 taccacccgc acggtgactc ggcggtctac gacaccatcg tgcgcatggc gcagcccttc
     7621 tcgatgcgct acgtgctggt cgacggacag ggcaacttcg gctcggtcga cggcgactcg
     7681 gcggcggcga tgcgctacac cgagatccgc atgcgcaaga tcgcgcacga gctgatcgcc
     7741 gacctcgaca aggaaaccgt ggatttcgtg ccgaactacg acggcaccga gatgatcccc
     7801 gccgtgttcc ccacgcgcat cccgaacctg ctggtcaacg gctcctcggg catcgcggtc
     7861 ggcatggcga ccaatatccc gccgcacaac ctcggtgaag tggtacgcgc ctgcctggcg
     7921 ttgatcgaca acccggagct cgagatcgat gacctgatcg agatcatccc ggggccggac
     7981 ttcccgaccg gcggcatcat caacggccgc cagggcatcg tcgaggccta ccgcaccggg
     8041 cgcgggcgca tctacgtgcg cgcgcgcgcc gaggtgatca ccgacgacag cagcggtcgc
     8101 gacacgatca tcatcaccga gattccctac cagctgaaca aggcgcggct gatcgagaag
     8161 atcgccgagc tggtcaagga aaagcgtatc gagggcatct cggagctgcg cgacgagtcc
     8221 gacaaggacg gcctgcgcgt ggtgatcgag ttgcgccgcg gcgagtcggg cgaggtggtg
     8281 ctgaacaacc tctatgccca gacccagctc gagagcgtgt tcggcatcaa cgtggtggcg
     8341 ctggtcgacg gccagccgcg cacgctgaac ctcaaggagc tgctggaggc cttcctgcgc
     8401 caccgccgcg aggtggtgac gcggcgcacc gtgtacctgc tgcgcaaggc gcgcgagcgc
     8461 ggccacgtgc tggaaggtct cgcgatcgcg ctggccaaca tcgacgcggt gatcgagctg
     8521 atccgcaatt cgtccaaccc cggcgaggcg cgcgaacgcc tggtggcgcg cgcctgggag
     8581 ccgggctcgg tgctggtgat gctcgacaag gccggccccg atgcctgccg ccccgaggac
     8641 ctgccgcgcg aattcgggct gcgcgagggc ggctaccacc tctcgcccga gcaggcacag
     8701 gcgatccttg acctgcgcct gcaccgcctg accggcctgg agcacgagaa gctgctcgag
     8761 gaataccagc agaagctcgc cgagatcgcc gactatctcg agatcctcgg cgacccgggg
     8821 cgcctgatgc gcgtgatccg cgaggagctc gagaaggtcg ttgccgacta cggcgacaag
     8881 cgcctcaccg agatcgtctc cagccggctc gatctcacgc gcgaggacct gatcccccag
     8941 gaagagcgcg tggtgacggt gtcgttcagc ggttacgcca agtcgcagcc gctcgatgcc
     9001 taccagtcgc agcgccgcgg cggtgtcggc cgctcggggg ctccggtgcg cgaggaggac
     9061 tcgatcgagc acctgctggt ggccaattcg caccacacga tcctgtgctt ctccgatcgc
     9121 gggcgcgtgt tctggctcaa ggtctacgag atcccggtcg cctcgcgcgg tgccaagggg
     9181 cggccgctgg tgaacctgct gaagctggaa ggcgacgagc gcatcacctc gctgttgccg
     9241 gtcggctcct tcgacgccga gcgcttcgtg ttcatggcga cctcggacgg caccgtgaag
     9301 aagacgccgc tcgacgcctt ctcgcgtccg cgcggcaacg ggctgatcgc catcgacctc
     9361 ggcgaggacg accacctggt cggcaccgcg atcaccgacg gcaacagcga cgtgctgctg
     9421 ttcagcagcg gcggcaaggc ggtgcgtttc agggaatccg aggtgcgcgc catggggcgc
     9481 aacgcgcacg gcgtgcgcgg catgcgcctg ccggccgggc agcgcgtggt gtccctgatc
     9541 gtgcccgacg acagcgcctc gcacgtgctg acgctgagcg ccaacggtta cggcaagcgc
     9601 accgtcaccg ccgagttccc gcgccattcc cgcggcggcc agggcgtgat cgcgatgcag
     9661 gcgagcgagc gcaatggcgc gctggtgggc gcgatccgcg tgctgccgag cgacgagatg
     9721 atgctgatca gcgaccaggg cacgctgctg cgcacggtgg tggaccagat ctcgctgctg
     9781 agccgcaaca cccagggcgt gaaggtcatg aacgtccgtg acggcgagac gctggtcggc
     9841 atggcccgca ttgccgaggc cgagggcccg gtggcggcgc acgaggacga ggacggcgcc
     9901 agcgccgcgg atcccgcgga cggctgaggc gcattttttc tttcgcacgc aacgaggaag
     9961 gcggagccat gacacgagta ttcaatttct gcgcgggacc ggccgcgctg cccgaggcgg
    10021 tgctggcgcg cgcgcgcgac gagctgctcg actggcacgg acgcggcctc tcggtgatgg
    10081 aaatgagcca ccgcggcaag gacatggtcg ccatcgcgga gaaggccgag gcggacctgc
    10141 gcgagctgct aggcatcagc gacgactacg cggtgctgtt cctgcagggc ggcgcgacgg
    10201 cgcagttcgc cgcggtgccg ctgaacctgc tcggcgcggc cggcaaggcc gattacgtga
    10261 acaccggaca gtggtcggtc aaggccatcg ccgaggcgca gaagtactgc gaggtcaacg
    10321 tggcggcgag ctcggaagcc acgcgcttca ccggcgtgcc ggcgcagtcg gtatggcagc
    10381 gctcgccggc ggccgcctac ctgcactaca cgcccaacga gaccatcggc ggcgtcgagt
    10441 acggctgggt gccggaggtg ggcgccacgc cgctggtcgc cgatatgtcc tcgaccatcc
    10501 tgtcgcgccc gatcgacgtg aaccgcttcg gcgtgatcta tgccggcgcg cagaagaaca
    10561 tcggtccggc ggggctggtg ctcgtgatcg tgcgccgtga cctgctcggg cggcccgacc
    10621 cgcgcacgcc cgccgtgctc gactggacgc tgcaggcgaa gaacgcctcg atgtacaaca
    10681 cgccgcccac ctattccatc tacctcgccg ggctggtgtt ccagtggctg aaggaccagg
    10741 gcgggctcgc cgcgatgggc gaggtcaacc ggcgcaaggc cgaggcgctg tacggctata
    10801 tcgacggcag cggcttctat gccaacccgg tcgagaaagc ctcgcgctcg tggatgaacg
    10861 tgccgttcac ggtggccgac gccgcgctcg agaagccctt cgtcgccgag gcggaaaaag
    10921 cgggcctgct gtacctcgag ggccaccgct cggtcggcgg catgcgcgcg agcatctaca
    10981 acgcggtcgg catggacgcg gtgaccgcgc tgatcgactt catgaaggat ttcgcggccc
    11041 gcaagggctg agcacggcat gcccgagatg acggattccg ccagcgcgct cgatcgcatc
    11101 cgcgccgaga tcgacggcat cgacctgcag ctgcaggcgc tgctgaaccg ccgtgccgcg
    11161 tgcgcgcagc aggtcgccga ggtgaagaag gccgagctgc tggcggcaca tggccacgtg
    11221 gagccggggc aggtgaactt ctaccgcccc gagcgcgagg cgcaagtgct gcgccgcgtg
    11281 atggcgcgca acgacgggcc gctgccggcg accgaggtcg cgcacatctt ccgcgagatc
    11341 atgtcggcct gcctcgcgct cgagaagccg atgaccatcg cgttcctggg ccccgagggc
    11401 acgttcaccc aggccgcggc ggtgaagcat ttcggttacg cggtgctgag ccgtccgctg
    11461 gcgacgatcg gcgcggtgtt cgaggaagtc gagtcgggcg agtcggacta cggcgtggtg
    11521 ccggtggaga attccaccga gggcatggtc acgcacacgc tggacaactt cgtgcgctcg
    11581 acgctgaaga tctgcggcga agtcgagctg ccaatccggc atcacctgct gggcgcgccc
    11641 ggctgccggc tggaaaacgt cacgcgcatc tgcgcccacc agcaggcgct tgcccagtgc
    11701 cgcgcctggc tggagaagaa ctggccgcgc atcgagcagc aggcgacgtc gagcaacgcc
    11761 gaggcggcgc gcctggcggc ggccgatgcc acggtcgccg cgattgccgg cgacatggcg
    11821 cgcgagacct acggcctggt gacgctggcg gcaggcatcc aggactacgc cgacaacacc
    11881 acgcgcttcc tcgtggtcgg gcgcgagatg gtcaagccga gcggcagcga caagacctcg
    11941 atcatcgtct ccacgcacaa caagcccggc gcgctgttca agctgctcga accgttccag
    12001 cgcgagcagc tgatgctgac gcgcatcgat acgcggccct ctcgttccca gccctggacc
    12061 tacctcttct tcatcgaatt cgaggggcac caggatgatc cgaaggtggc ggccgtgctg
    12121 gccgagctgg aggagcagac ggtgatgctg aagatcctcg gctcctatcc ccaggcagtg
    12181 atctgaggcc atgagcgcgc gcgtgggcac gctgctcttc gtcggcctgg gcctgatcgg
    12241 cggctcgctg gccgctgcgc tgcgcggcac cggcttcgcc gcccgcatga cggcgttcaa
    12301 ccgcaatcgc gccacgctcg atttcgcgct cggcgccggt ctcatcgatg ccgcgccggc
    12361 gagcctcgag gaggccattg ccgaagccga cctcatcgtg gtcggcgtgc ccacgctgag
    12421 cgtgggccgg atcttcgcgg cgatccgcgc gcacgcgcgc ccggatgtgc tgatcaccga
    12481 cgtggccagc gtcaagggca gcgtggtggc ggccgcgcgc gaggccttcg gcgcggtgcc
    12541 gccgcggctc atcccggggc acccgatcgc cggcgcggag aaatccggcg tcgaggcggc
    12601 gcgcgcggat ctcttcgtgc gccatcgcgt gatcctgacg ccgctcccgg agtcggatcc
    12661 cgaggcgctc gcgacgatcg acgccatgtg gcggctgacc ggcgccgagg tggtgcgcat
    12721 ggacgtggcc gcgcacgacc gcgtgctcgc catgaccagc cacctgccgc acctgctcgc
    12781 cttcgccctg gtcgacagcc tggccaggca gccggcgagc gaggagatat tccgctgcgc
    12841 ggccgggggt ttccgcgact tcacgcgcat cgcctccagc gacgtggtga tgtggcgcga
    12901 catcgcgctg gccaaccgcg atgcgttgct cggggcgctg gatggcttca cgcacacgct
    12961 ggacgccgtg cgcgcggcgg tggcgagcgg cgacggcgag gagctggaac aggtgttcgg
    13021 gcgtgcgcgc gcggcgcgcg agcgctacct gcgctggctc gaacggggcg aatcgcgctg
    13081 aattcttgct ggacaatgac gggggcaaag tgacacacga cgggcgaatc gattacatcg
    13141 cgagcggcgg cgccgtgctg cgcggcgagc tgcgcgtgcc cggcgacaag tcgatctcgc
    13201 accgctcgat catgctgggc tcgctggccg agggcaccac cgaggtgagc ggattcctcg
    13261 aaggcgagga cagcctggcg acgctggcgg ccttccgcgc catgggcgtg cgcatcgagg
    13321 ggcccgagca ggggcgcgtg cgcatcgagg gcgtgggcat gcacggcctg cgcgcgcccg
    13381 cggctgcgct ctacctcggc aattccggca cctcgatgcg gctgctggcc ggcctgttgt
    13441 cggcgcagcg cttcgacgtc tgcctgagcg gcgacgaatc gctgacgcgc cgcccgatgg
    13501 cgcgcgtggc ggatccgctg cgccaaatgg gtgcgcagat cgagaccgcc gccaacggac
    13561 gcccgccgct gtggctgcgt ggcggccacg cgctgcacgg catcgagtac cacatgccgg
    13621 tggccagcgc gcaggtcaag tccgcggtgc tgctggcggg gctctatgcc gcgggcacca
    13681 cgcgcgtgat cgagccggcg ccgacccgcg accataccga gcgcatgctg agcggcttcg
    13741 gctacccggt gctgcgcgac ggcgcgcgcg tggaactggt cggcgggcag agcctgcgcg
    13801 cgacgcgcgt cgatgtgccc gcggacatct cctcggcggc ttttttcctg gtcgcggcgg
    13861 ccatcacgcc gggttcggaa ctgctgctga agaacgtggg cgtcaacccg acgcgcaccg
    13921 gcgtgatcaa catcctgcgg gcgatgggcg cgagcatcga gctcctcgat ccgcgcgagt
    13981 ccggcggcga gccggtcgcg gatctgcgcg tgcgcgccgc gccgctgcgc ggcatcgaga
    14041 tcccgctgga gcaggtgccg ctggcgatcg acgaattccc ggcgctgttc atcgccgcgg
    14101 cctgcgccga gggcgagacg gtgctgcgcg gcgccgagga actgcgggtc aaggaaagcg
    14161 accgcatcga tgcgatggcg cgcgggttcg aggcgctggg tgtgcggcac gaaacgctgc
    14221 ccgacggcat ccgcatccgt ggcggggcga tcggcggcgg caccatcgac agccgcggcg
    14281 accaccgcat cgcgatggcc ttcgcggttg cctcgctgcg cgccagcgcc ccgatcgaga
    14341 tcctcgactg cgccaacgtc gccacctcgt ttccgggctt tcgcgaactc gcggcggcgg
    14401 ccggactgac cgtcactcat aaagcgacgg gcgcctgagc gtccgcgcgc cgcagaccaa
    14461 ggatagaaat gattaccgaa gaagacattc cggtcatcgc cgtcgacggg ccgagcggct
    14521 cgggcaaggg caccgtctgc cggttgctgg cccgcgagct gggctggaac ctgctcgaca
    14581 gcggtgccat gtaccgcctg gtcgggctgg ccgcgcgcaa ccacggcgtg cggctggaca
    14641 accacgccgc gctcgacgtg ctggccgggc acctcgatgt gcagttccgc cccggcgagg
    14701 acaccgccga ggcgcgcgtg atcctggagg gcgaggacgt gagccagctg ctgcgcaccg
    14761 aggaggtggg cgcgctcgcc tccgaggttg ccgcggtgcc cgcggtgcgc gcttcgctgc
    14821 tgcggcgcca gcgcgcgttc aagatccgtc ccgggctggt ggcggacggg cgcgacatgg
    14881 gcaccgtggt gttcccggag gcgcagttca aggtgttcct gaccgcgagc gccgacgagc
    14941 gcgcccggcg ccggtataag cagttgaaag ataagggaga agctgttagc ctgccgcgcc
    15001 tcgtggcaga catcgaagcg cgcgaccggc gcgataccga gcgttccgtg gcgccgctca
    15061 gggcggcgcc cgatgcggtg acggtcgata ccacggggat gggcatccag gaagtcttcg
    15121 atctcctgat ggccgaagtg aaggaacggg gcatgctctg aagccccgga tgcggcggcg
    15181 tcataccctg tggcgccacg cacggctcca gttgccgggg atttctgtcc agcatcccgg
    15241 ccgctggttt ttttattttg accctgagcc ggctggggcg agggcaagac catacgagcg
    15301 gcaagtccgc tgcaggatat tccaatgacc gagaattttg cgcagctttt cgaagagagc
    15361 ctgaagaccg ttgagatgga cgtcggctcc atcatcaccg gcgtggtcat cgacatcgac
    15421 aacgagtggg tcaccgtgca cgccggactg aagtccgagg gcgtgatccc gcgcgtgcag
    15481 ttcctcgacg acaacggcga gttccacctc gagatcggcg acgaagtgta cgtcgcgctc
    15541 gaggccatcg aggacggcaa cggcgaaacc cgcctgtcgc gcgaaaaggc tcgccgtgcc
    15601 gagacctgga aggccctcga gggctccttc gagaacagcg aagtggtgcg cggcgcgatc
    15661 aacggcaagg tcaagggcgg cttcacggtc gacatccagg gcatccgcgc gttcctgccg
    15721 ggttcgctgg tcgacgtgcg cccggtgcgc gacaccgcgc atctcgaagg ccagacgctg
    15781 gatttcaagg tcatcaagct cgaccagaag cgcaacaaca tcgtggtttc gcgccgcgcc
    15841 gtgctcgagc aggcgaacag cgccgagcgc gaatcgctgc tggcaaacct gcaggaaggc
    15901 gcaacgttga agggcgtggt caagaacctc accgactacg gcgccttcgt ggatctcggc
    15961 ggcgtggacg gcctgctgca catcaccgac atggcatggc gtcgcatcaa gcacccgagc
    16021 gaagtggtga aggtgggcga cgagatcgac gtgaaggtcc tgaagttcga ccgcgagacg
    16081 aaccgcgtct cgctcggcct gaagcagctg ggctccgatc cgtgggtcaa catcaaggcc
    16141 cgctaccccg agaacgcccg cgtcaaggcg cgcgtcacca acctcaccga ttacggctgc
    16201 ttcgcggagc tcgagcaggg cgtggaaggc ctggtgcacg tctcggagat ggactggacc
    16261 aacaagaaca tccatccctc gaaagtggtg caggtcggcg acgaggtgga cgtgatgatc
    16321 ctcgacatcg acgaggaacg tcgccgcatc tcgctcggca tgaagcagtg ctccgagaac
    16381 ccgtgggacg cgtttgcctc gacccacacc aagggcgagc gcatcagcgg caagatcaag
    16441 tcgatcaccg acttcggcct gttcatcggc ctcgacggca acatcgacgg cctggtgcac
    16501 ctgtcggaca tctcctggca cgaaaccggc gaggaagcgg tacgcgcgta caagaagggc
    16561 gatgacgtca acaccgtcat cctgtcgatc gaccccgagc gcgagcgtat ttcgctcggc
    16621 atcaagcagc tcgaggacga tcccttcaac aatttcgtcg ccatcaacga caagggctcg
    16681 atcgtgaccg gaaaggtcgt cgacgtcgat gccaagggcg ccgtgatcga gctggccgag
    16741 gacgtgcagg gcacgctgaa ggcctccgag atcagccgcg acaagatcga ggatgcgcgc
    16801 aacgtgctga agatcggcga ctcggtggaa gcgaagatca tcaacgtgga tcgcaagaac
    16861 cgcgtgatcg ggctttcggt caagtcgaag gacgtgcagg acgagaagga ggccatcaag
    16921 gcactcaagg aagagtccga ggcggcgcct gccaccacca tcggggacct gatcaaggcc
    16981 cagatggaac gcaagtaaca cgccgcacga agcggggccc cagggccccg ctttcgtttc
    17041 cggcctgccg cgggtgcggc cagccccgtg cgttagacgc gagtcccgat ctggcctatt
    17101 attctggctg tctgcttttt gcaataaaaa cagcgggttg aacatgacga agtcggagct
    17161 catcgaacgc atcgcgacgc agcagaagca gctgtcgccg aaggacgtcg aactcgccgt
    17221 caagatgatc atcgagtaca tggcgcagag cctgtcgacg ggggatcgta tcgagatccg
    17281 cggcttcggc agtttctcgc tccactatcg cgagccccgg cgcgggcgca accccaagac
    17341 cggcgatgcg gtcgacctgc tgggcaagta cgttccctac ttcaagccgg gcaaggagat
    17401 gcgcgagcgc gtgaatctca atgccgacca ggaaatcagc ggctgagacg ccggtgatga
    17461 aacgtctcaa gcgtttgctg ctgctggcac tggtgctggg cgtcgggctg tgggccttcc
    17521 tgttttccct cgccaacagc gagccggtga cgctggacct ggtgttcgcc cgcctggagc
    17581 cggcagcgct ctccgtgtgg ctgatcgcgg ctttcgtcgg gggcggcttg tgcgggctgt
    17641 tcgcgggcag cgtggcgatc tggcgtgcgc agcgtgccct cccgcgctcg cggcgcgtac
    17701 ccgcggcgcc ggcggtcgtg gcgccacccg cgggcacaac tgcggcaacg cgctgagcac
    17761 ggcgtctcca tccatcaccg gcgctggcgc ggcgacgcgg gcggcgtttc cgaggtctcg
    17821 catgcaatct ccatccggct cccctgatcg ctgcgccgac gccggcccgc gcatcgtcgt
    17881 ggcactggac tacgccaccg cggcggcggc gctggcgatg gccgggcggc tcgacccgcg
    17941 ccgttgccgg ctcaaggtcg gcaaggagct gttcacggcc gcaggcccgg agctggtgcg
    18001 cagcctggtc gcgcgcggct tcgaggtgtt cctggatctc aagttccatg acatacccaa
    18061 cacggttgcc ggcgcggtgc gcgcggccgc ggagctgggc gtgtggatgg tgaacgtcca
    18121 cgccggcggc ggccggcgca tgatgcaggg cgcgcgcgcc gcgctcgctt cgctgcccgg
    18181 gccgcggccg ctgctgatcg ccgtcaccgt gctcaccagc atggaagccg acgagctcgc
    18241 ggaaaccggt gtgagcggcg ggctggagca gcaggtgctg cgtctggcgg ggctcgcgct
    18301 cgagtgcggg ctcgatggcg tggtctgctc ggcgcgcgag gcgccgctgc tgcgcagcca
    18361 tttcggtgcc gcgccggtgc tggtcacgcc cggcatccgg ccggccagcg ccgcccccga
    18421 cgaccagcgc cgcacgctga cgccggccga ggctctggcc gcgggcagcg attacctggt
    18481 ggtggggcgc ccgatcaccg ctgccgccga tccgggcgcg gcgctggagg cgattctgtc
    18541 cgacatttcc tacagattct cctgaatggg catgctagcg tttgttcacg gtgcaacgga
    18601 tgcaccgtgt catgtcccga caatcaagga ggattccaat gaaactcgca gacgttctga
    18661 aggcatcggt actggggctc tcgctgctcg tgctgccggc ggctcacgcc gccgcggagc
    18721 aggccagtgc gggcggcgcg ccggtgcagc aggccagcca gcaggtcaac atcaacaccg
    18781 cggatgtcgc cacgctcgcc gggtcgctgc gcggcgtggg tatcaagaag gccgaggcga
    18841 tcgtggcgta tcgcagcgag cacggtccgt tcaagtccgt ggatgagctg gtcaatgtca
    18901 agggtatcgg tgacaagatg ctggagcagt tgcgctcgca agtgtcgctc tgagcgctcg
    18961 ccagggcatc gccgggggcg cccgcgggcg ccccttcctt tttgttcacg cgcatcgtgc
    19021 ccggtgcgcc tgccggcagg ccgccgccaa tgagccgtat cgcgctcgtc accgggggcg
    19081 gcggctacat cggttcggcg ttgtgtcgcg cactgcaggg gcggggctgg cgcgtgcttg
    19141 ccacggcgcg cgatccggcc atcgtgacgg cgacaccgtg catcgcgctg gaactcgagg
    19201 atgccgcggc cgggcacgtg gatccgcagg tgtgtcggga tgccgaggtg gtattccacc
    19261 tggccggcat cgcgcaccgc ggcgccgatg cgctcgcgct cgagcgcatc aactgcgatg
    19321 ccacgctggc gctcgcgcgc gcggcagaag cggccggagt cgcgcatttc gtctatctca
    19381 gcagcctgta cgcgggcgcc tgcgatccgg gggcgcctgg cgcgcgcggc gacgcctacg
    19441 cgcgctcgaa gcggctggcc gagctgggcc tgctcggcgc cgagcccaac tggcgcatgc
    19501 gcgtgacgat cgtgcgcccg gcgctggtct acggtggcgc gatgaagggg cgcctcgcca
    19561 cgctgcgcgc gctcgcgctg aagggctggc tgcccgatct gccgcccgtg ggcaacacgc
    19621 cgatgatcgc gctgcccgac ctcgtgcacg cgctcgcgct gttgggcgcg catccgagca
    19681 tcagcggcaa catgttcacg gtgagcgacg gcgagcgcta cccgctggat cgcatcgcgc
    19741 acgcgatgca ccggctcgcg ggtcggggca gcggcgcgtg gcgcatcccg gcgggcccgt
    19801 tcgcgctggc gctggccgcg gcggcgcggc tcgggcgcct gcccgtgctc ggatctcgac
    19861 tggccctgcc acgcggcctg gccgactgga cggtggatcc cgatcagcgc ctgcgcgcgc
    19921 tcggctgggc tcccgcgctc accctggaaa ccgcgcttgc accggccatg ccggatgggg
    19981 agcgcgcatg aggggcgagc actgatgcgc gctcttgccg tggttgccac cgccttcacg
    20041 gtggggtggt gcgtgacctg gtgctattgc cgctgggcgc tgcgtgccgg acatctcgac
    20101 gtgccgggcg cgcgcagttc gcatgccctt gccacgccga ccggcggcgg cgcgggcatc
    20161 gtggctggct gcgccgcggc gctcgtcgcg accatggccg gtggcgcggt gcacgcggac
    20221 tggttgctgg tgctttgcct tgcgctgctc atggcggcgc tgggatacgc cgatgaccgg
    20281 cgggccctgc cggtgctccc gcggctgctg gggcagttcg gcgcggccgc ggccgtgatg
    20341 gcctgcgcgc tggcggtggc cggcatcggg cccgggggcg cggggttgct gctcctgccg
    20401 ccggggctgc tcgcgctggt ctggctgaac aacgccttca atttcatgga cgggatcgac
    20461 ggtctcgccg cgtcccaggc cgtgttcatg gccggggcgg cggcgtggct ggtcgcgttc
    20521 ggcgccggtg cggacgactc ggtgctgctg ctcggcggca tcgccggggc ggcatcgggc
    20581 ttcctggcgt tgaactggcc gccggcgcgc atcttcatgg gcgacaccgg cagccagccg
    20641 ctgggcctgc tgctcggtgg cgccgcggcg gtgacggtgg cgcgcggcga gctctcgctg
    20701 gccgtgtggg caatcctgtg ggccgccttc ctcgccgacg ccacgctgac cctgctgcac
    20761 cgggcatggc ggcgccaacc gctgctgcag gcgcaccgcg atcacgccta ccagcggctg
    20821 gcgcggcgcc gcggcgcgca ccgcccggtg acgctgggct gcatcgcgct gaacctgtgc
    20881 tggctgctgc cgctggcatg gcttgcgcac cggatgcccc gtttgggcct ggtttgcgtg
    20941 ctgatcgcct acgttccggt ctgcctgggc gtgcttgcgg tgctgctgcg cgatgggcgc
    21001 gagtcccccg cggcatcgtc cggacgtcca gtgaaatagt gggcacgatt gagggcgtcg
    21061 cttcgcgcca tgccggtgcc catttgcgtt agcattcccg gtgccgtcga gaacaagagg
    21121 cacgggtggg gcgtggcgag agaagcgcga tgaatctgtt gttccgcatg ttgcgcgaag
    21181 ccgctcgggc cagcagcgcg ttcatcacgc tgctgtatga acgcttcgtc gtgctcccgc
    21241 acaacgtgaa gcaggcactg ctgctgctgg tcgacctggt gtccatcccg gtggccctgt
    21301 tcatcgcgac cacgctgcgc tacggcacgc tgcaagtgga gatcggctcc agtgaactcc
    21361 tggtctgtgc cgccacggtg ctggtctccg cggtggtgtt cctgcgcctg ggactctacc
    21421 gcgcgatcgt gcggttcatg ggccacgagg ccatcgtcgc catcgtcaag ggcgtcacca
    21481 tctcggcact gttgctggcg ctgttcacgc tgctgacgcg cgccgaggtg ccgcgctcgc
    21541 tgccgatgat ctactggtgc ctcatgctta tctgcgtggg cggctcgcgc ctggtgctgc
    21601 gttcctggta ccggcatcgc cgcgagcgca gcgcgcgtcg cattgccatc tatggtgccg
    21661 gttcggcggg ccgtcagctg ctgaccgcgc tgctgcacgg cgagcagttc gacccggtgc
    21721 tgttcatcga cgacaaccct gcgctgcgtg gccgggtgat caacgacgtg ccggtgatcg
    21781 ggctcgacca gctgcccgcg aagatccggc actccgggat cgccgagata ttcctcgcca
    21841 tggcatcgat cggcgccgat cgcaggcgcg agatcaccca ggcactcacc gagctcgccg
    21901 tgcacgtgcg cacgatcccg cgtttcgagg atctcgtgcg cgggcgcgcg gacatcagcg
    21961 aggtgcagga gatcacgcta gaggacctgc tcggtcgcga gccggtgccg ccgcgccccg
    22021 acctgctggc cgtctgcatc gccggcaaac gggtgatggt caccggcgcc ggcggctcga
    22081 tcggttccga gctgtgccgc cagatcctcg ccgaatcgcc cgctgagctg ctgctcgtcg
    22141 acgccagcga gttcgcgctc tacaacatcg agcgcgagct gctcgatgtg gccgcgcgcg
    22201 gggggcttgc cacgcgcatc gtcgcgctgc tcggttcggt gcaggaccac gcgcgcatgc
    22261 gcctgctgct cgagggtttc caggtccaga ccgtctatca cgcggcggcg tacaagcacg
    22321 tgccgctggt cgagcacaac gtcatcgagg gcgtgcgcaa caacgtgatg ggcacgctca
    22381 cggtggcgcg cgcggcggcc gaggccaagg tgcgcacctt cgtgctggtc tccaccgaca
    22441 aggccgtgcg gccgaccaac gtcatgggcg ccagcaagcg ccttgccgag atggtgatgc
    22501 agtcgctggc cgccgcgaac gccggcggca cgcgctacgt catcgtgcgt ttcggcaacg
    22561 tgatcggcag ttccggctcg gtggtgccgc tgttccagga gcagatcgcc gcgggcggac
    22621 cggtcacggt cacgcacgcc gaggccgagc gctatttcat gaccattccg gaagccgcgc
    22681 agctggtgct gcaggcgagc gcgatgggca gcgggggcga tgtgttcgtg ctggacatgg
    22741 gcgcgcgggt gcgcatccac gacctcgccc ggcgcatgat ccacctggcc ggcaaggaag
    22801 tgcgcgacga ggccaatccg ggcggggaca tcgagatccg catcaccggg ctgcgtcccg
    22861 gcgagaaact gcgcgaggaa ctgctgtccg agggcagcgt cggcgccacc gggcatccga
    22921 tgatcctgcg cgcggtcgag gaatgcctgc cgtgggccga gctcgagcgc gtgctcgagg
    22981 agctgagcga ggcctgcgtc gactatgatt gcaagcgggt gcgcgacatc ttcgcgcgcg
    23041 tggtgcgcgg tttcgacgac cgctacccgt gcgccgatgc gctgggcgag cggctgaacg
    23101 agcgcgcggc cggacagcgt ccgcgcctga agatgctggc gccggtgaag tccggctgag
    23161 ggagcttgcc ctcgcgcgcg ctggcgcgcc ggccaaagaa attgctgctg cctggaggaa
    23221 ggattcccga tgcgatacgt ggatgtgttc aatggcgatg ccgacggcat ctgcgcgctg
    23281 ctgcagctgc agctgagcag cccgcgcgac agcgagctgg tcaccggggt caagcgcgac
    23341 atcgacctgc tgcgccaggt gcgggccggc gcgggcgacc ggatcacggt gctcgacgtc
    23401 gccatggaga agaaccgcgc cgatctcgac cgcctgctcg cggcgggcgc cagcgtgttc
    23461 tacgtggacc atcacatccc gggcgagatc cccgaacacg cgggattgca ggccatcatc
    23521 aacaccgcgc cggaggtctg caccagcctg ctggtgaatg gccacctgaa gggcgcacac
    23581 gcgcgctggg cggtggtcgg cgctttcggc gacaacctgg gcgagagcgc gcggcgcctg
    23641 gcgacccaga tcgggctgga cggcgaggcg ctcgcggcgg ccgagaacct cggcaactac
    23701 atcaactaca acggttacgg cccgagcatc gaggatctgc acttcgcgcc ggccgagctg
    23761 ttccggcgcc tgcgccccta cgcggatccg ctcgccttca tcgccgggga ccgcgagacc
    23821 ttcggcaggc tcgaggaggg ctttcacggc gatatgggca tggcggcggc cctgaaaccg
    23881 atgcacgagt ccgcgagcgc ggcggtgttc ctgctgcccg acgagccctg ggcgcggcgc
    23941 gtgagcggcg tctacagcaa tgacctggtg aacctgcatc cggcgcgcgc gcacgcggtg
    24001 ctcaccgaga tcggcggcgg cgatctgctg gtgagcgtgc gcgcgccgct cgccaaccgc
    24061 accggcgccg acgaggtgtg ccggcagttc gccaccggcg gcgggcgcaa ggcggccgcg
    24121 gggatcaacc ggctggcgcg cgaggatctc gcacgcttca tcgacgtgtt ctgctccttc
    24181 tacgcggcgc acgccgggag ctgaggggcg tgccgacggc atggcccgcc cacatgccag
    24241 cgcccggcgc catgcggcgg cagacggtgt tgcgcctgct catgctgctg tggtgcgtag
    24301 cgcccgaagc gacgcgagcc gaaggggaaa tccaggtcgg cgctccgcgc tgccagcctt
    24361 cgcagggttg cgaaactgcc gaggcgcaag cgccggcgca gcactgggtc gacacctcgt
    24421 acgcctacgt gacctcgcgc accgattcgc tggcggtgtg gctggattcc ttcttcggca
    24481 cggtgacctc ggacatggag tccgccgact cggtgttgcg cttgcgcacc gagtacgagt
    24541 ggaacgagga ggacggcgag gactacaagg tgcgtctgcg cggcaaggtc gatctgccgc
    24601 gccttgacga gcgcctcagc ctggtcttct ccgaagaggg cgaggaacgc gaggaagtga
    24661 ttccgaattc ggccggcaac gacgacgacg tcggcctgca gttccgggtg cgcgagcgga
    24721 gcagatcgcg tctgtggttc acggtcggca ccaattcctc gctcgatttc aagtcgagcg
    24781 tgcgctaccg ctacatccag cccatcggag acgactggcg cgtgcagtac acgcagcggt
    24841 tccacttcaa ggagggtgac ggttttggca ccaccacgcg tggcgatctc gactacctga
    24901 tatcccgcga tcgcatcgtg cgctggacca accaggtgga gtacggcgag gagaccaacg
    24961 gcgcggagtg gggcacgcgg ctcagctacc agttgcgtct cagcgagaag gacgccttga
    25021 gctacttcgg cgcggtcgcc ggggaaaccg atcccgagaa cctgacccag agctacgccc
    25081 tcggcgtgcg gtaccggcgc aacttcctgc ggccctggat cttcttcgag atcgagccct
    25141 cctacgcctg gcggcgcgag tcggtggagg agtcgcgcag ctccctctgg ctgctgacgc
    25201 tgcgcctgga attcctcgag gagaggtaca accgccgcgg gcacgacgag gcgaaccgcc
    25261 aaaccgtcga ctagactgac gggggtcaaa gccgccgccg ggccggctcg catcgcctgt
    25321 ggtgcgggag gggtcggcaa cgaccgtcgg accgccagat gtcacagttg cacgccgttc
    25381 ccaccgctgt caccgatctc gagatcggca tgttcgtcac cgcgctcgat cgtccgtgga
    25441 tcgagacccc gttcctggtc gaaggcttct acgtcaccag ccaggcggac atcgacgaag
    25501 tcgagaagca ttgcaagttc gtctatgtcg atgtctatcg ctgccggctc ggggcgggcc
    25561 ggcgcaacag ccgcgtggtg ggtgcggccg gtcaaaaggg ggcgacgata ccgctgccgc
    25621 aggagccggc gctcgagacg atcaagttcc gccagcgcag cgccaccgcc atcgccgaga
    25681 tgttcccgca ccgcaagctg aagcccttcg aggacagcag cacctttggc ggggagctgg
    25741 cggtggccag gccggtgtat gccgaggtgc tgcgcgcgta ccggcagatg atggaggcgt
    25801 accgcgccgg gggcaggctc gacgtctcgg gcgtgcgcga ggtcgtcaac ccgatggtcg
    25861 agagcgtgat ccgcaatccc gacgcctgtg tctggctggc gtgcagcaag gacgagcaga
    25921 gctacacctg gggccattcc gccggcgcct cggtgtgggc cgtggcgctg gggcgccagc
    25981 tcgggctgcc gcgcgtggac ctgcagcgcc tcgccatggg cgcgctgctg ctggacgtgg
    26041 gcaagctgaa ggttcccgag gcgctgctgc tcaagcgcga gcgcctgacg ccgggtgagt
    26101 tccagctgat caagtcgcac gtggagttcg ggctgcagat gctggaggga accgggatcc
    26161 tcagccgcag tgtcaccgac atgctggagt tccaccacga acggcacggg ggacacggtt
    26221 atccgcgcgg catcgccggc aacgacatcc cggtcttcgg gcgcatcgcc gcgatcgtcg
    26281 actgctacga cgccatcacc agccgccgtc cctacgcacc accgatgtcg ccgagccacg
    26341 cggtgaaaca gctctacgcc tggcgcgaca tcgatttcga cgccgagctg gtcgaggagt
    26401 tcatccaggc catcggcatc tacccggcag gcacgctggt cgagctcagc aacggcgagg
    26461 tgggcgtggc gacggcgggt taccgcacgc gccgcctgcg cccgcagctg ctgctggtgc
    26521 tggatcgcga caagaaaccc tacgcctcgc cgcgcccgct cgacctgctc gcggtcacgc
    26581 acgaccagga cggcaagccg ctcgagatcg ccaccagcct ggagccgggc tccttcggcg
    26641 tggatcccgt caccctggtc gtctgaaccc gctttcgcgc cgttggcgcg tcggcgcgcc
    26701 cgagccactc cccgcagcac tgtccattgc gcccctggcg attcccgcga gggctgcgcc
    26761 gtcgtgattg tgtcagttgc gttacacgca ccggggcagt tgcggttgcc tcgccgtgcc
    26821 gatgtcacga ttctgtcaca tgccaacccg agaatgcccc cgtcgtggca gccatcgccg
    26881 gcagcctcgc cctgggcatc aaagctcact ccctcgcaca caggggagcc cagtccatca
    26941 cagcaaggag acagagaatg cgtttatcga aagcagttgt atgcggaggt ttgattctcg
    27001 cgggggccac ccaggccaat gccggctcgg tcgatgcgaa gctgctcgac atgctgaagg
    27061 caaatggttc catcacggcg gcacagcacg ccgagttgag cgctgacctg gtgcgcgaga
    27121 cacgcgagga gaagcgcgcg gccaaggaag tggtcaagga gaaggatttc gtcgcattcc
    27181 gtcaggcagc cggctgggcc gagagcacga agctcacggg tgatatgcgc gtgcgccagg
    27241 aaacgatcga tgtcgacggc gagcccgact tcaccggcag ccgcaacaag gaccgccagc
    27301 gcatccgtgc ccgtctcggt gcgttcacca agatcaatcc cgaagtggaa accggcatcc
    27361 aggtcgcgag cggcaacggc gccgaccgtc gctcgaccaa cgagaacatg gacaattact
    27421 tcgacaagaa ggcggtgtgg ctggacctgg cctacatcaa ctacaacccc gtggcagtgc
    27481 ccggcctgaa gaccttcgcg ggcaagatga agcagccgtg gatgtcgatg ggcgacgtga
    27541 tctgggacgg tgacatcaac cccgagggcg tggcggcggg ctacacgaag aagaacggca
    27601 ccactacgtt cttcggcagc ggcggctact acgtgctgaa ggatgccgtg gacagcgaag
    27661 gcttcgagat cgagaacgac ctcgcgctgt accagttgca gggcggcgtt gcgttcgacg
    27721 tgagcgattc ggcgcgctgg accctcggcg ccagcgtcta cgacttcaac aacgacaagt
    27781 acggctcgac ctcctcgttc cgtgccaacg gcaacaccac cgacaagttc ggcctgggcg
    27841 aaatcttcac ccagctcgac gtgatcggac tgccgctgcc gctgtcactg tacggccagt
    27901 acgtgcagaa ctacgaggcg cgcgacatgg ccgcgaccac gatcgacgac agcgacgagg
    27961 acacggcgta cctgttcggc ctgcgcacca acatcgcggg cgtggcgttc gactacaact
    28021 accgtgacgt ggatgccaat ggcgtcgtgg gtggcttcac ggattccgat ttcgccgtgg
    28081 gttacacgaa ctccagcggt cacaagctga agttgaagta cgacttcatg aaaaacttct
    28141 cgatcggcgc gacctatttc atggccgaat ccgacgccgt gagcagccgg aagctgaagg
    28201 gcgtggacgc ggacaccctg cacgtcgatc tcgaagccaa gttctgatgc gcccggcgcc
    28261 gtccgcgcga gcgggcggcg ccggattcgc gactgcacgt tccaaatacc agtggagtac
    28321 atgtgatgaa gaagatttcc ggatccttga aggcggccat cggcgtcgcg gcgctgtgcg
    28381 gcggtttcct gtcgcagcag gccggcgcgg ccagcgtgat cgagatcgac ggctccagca
    28441 ccgtctaccc gatcaccgag gcggtggccg aggaatacca ggctgcgggc ggtgcgcgcg
    28501 tgacggtcgg catctccggc acgggcggcg gcttcaagcg cttctgccgc ggcgagaccg
    28561 acatctcgga cgcctcgcgc ccgatcctga aggaagagat ggtgctctgc gcgcaggccg
    28621 gcatcaagta tttcgagctg ccggtggcct atgacgccat cacggtcgtg gtcagcaagg
    28681 acaacgattt cgtcgaccac ctgacggtcg aggaattgaa gaagatgtgg gagccggccg
    28741 cggcgggcaa gatcaccagc tggaagcagg tccgtgacag tttccccgac gtgcccctga
    28801 agctcgccgg cgccggtgcc gactcgggca ccttcgacta cttcaccgag gcggtcgtgg
    28861 gtgttgccaa gtcttcgcgc ggcgattacc aggccagcga ggacgacaac gtcaccgtgc
    28921 agttcgtctc gcgcgacaag ggcgcgctcg gctatttcgg cctggcgtac tacgaggaga
    28981 acaaggacac gctgcgcgcg gtgccgatcg tggctcccgg cggttcgacc ggcgtggtgc
    29041 cctcgatcgc caccgtgaac gacggcacct acactccgct gtcgcgcccg atcttcattt
    29101 acgtgaagga gaagtcggtg cagcgtccgg aagtgcgcaa gttcgtggag ttctacttga
    29161 aggaaggcgg tgcgctgacg cgtgaagtgg gcttcgtcga cctgcccgcg gcggcctatg
    29221 agttggccct caagaacttc aacgaaggca agctcggcag cggcttcggc ggcaagtcgg
    29281 aagtcggcct gaaggtcgag gagctgctgg cccgcgaagg caagctctga tcggttcccc
    29341 tgaggaagtt aggggtggtg cgtggcgctc agccgccacc accccgtttt tttccgggca
    29401 ttctccgcta tccatttccg acggatccat gccatgactc ctaacagcgc caagccagca
    29461 gcggcagaga cataccctgg caaccgcttc gcgcaccgcc cgctccggca tgtcagcgag
    29521 cggctgatcg agggcgtgtt gtttctcgcc gccttcagcg ctgtggcggt cacggtagcc
    29581 atcgtattca tcctcgtggc cgagtccgcg gccttcttcg agcacgttcc gctcatcgat
    29641 ttcctcaccg acacgcagtg gaccatcatg ttcagcgaga agcactacgg cgtggcgccg
    29701 ttgctcgcgg gcacgctggt gaccaccacg gtcgggctgc tgctggccgt gccggtgggc
    29761 acgatcaccg cgatctacct cagcgagttc gccgggccgc gcacgcgcga gatcgtgaaa
    29821 cccgtgctgg agctgctggc cggcatcccg accgtggtct acggctactt cgcgctggtc
    29881 atggtcacgc ccgcgctgca gtacttcatg ccggacctgc ccggcttcaa catgctcggg
    29941 ccgggcctgg tgatcggcat catgatcatt ccctacatca gctccgtggc cgaggacgcg
    30001 atgcgcgcgg tgccgctgca catgcgcgag ggttcctacg cgatgggcgc aacgcggctg
    30061 cagacggcgc tgcgcgtggt ggtgcccgcg gcgacctcgg gcatcgtcgc ggcctacatc
    30121 ctcggcatat cgcgcgccat cggcgaaacg atggtggtcg cggtggcggc cgggcagcag
    30181 cccaacttca cctgggatcc gcgcgaaccc ggcgccacga tcaccgcgtt catcgtgcag
    30241 gtggcgctgg gtgacctgcc gcacggcagc atcgagtacc agagcatctt cgccgtcggc
    30301 ctggtgctgg tgctgatgac gctgttcttc aacgtgctcg gccacctggc acgcaagcgc
    30361 tttcgcgagg cctactgatg acacaggaca tgcagcagat ccgccgcctg gttgcgcgcg
    30421 gcaagcagtg ggacaaggtg ttcgtgatca tcgggctgat atgcctgatg ataggcgtgc
    30481 tgacgctggc ggtgctgctc gccgggctcg tccaggacgg ggcgggccga ctgaactggg
    30541 acttcttcac cagcttcccc tcgcggcgcg ccgagaatgc cggcatcctc agtgccatcg
    30601 tggggtccag cctggtgatg ctgctgaccg cgattaccgc gatcccgctg ggcgtcgcgg
    30661 cgggcgtcta tctcgaggag tacgcgcggc gcaactgggt gacggagatc atcgagatca
    30721 acgtgaacaa cctcgcgggc gtgccctcga tcatctacgg cctgctcgcg ctggggctgt
    30781 tcgtctacgc gctcgatttc ggccagagca tcctctcggc cggccttacg ctggcgctgc
    30841 tgatcctgcc gatcatcatc gtggcgacgc gcgaggcgat acgctcgatt ccatcgagca
    30901 tccgcgaggg cgcctacgcg ctgggggcca cgcgctggca gatggtgc
//
LOCUS       DAORUQ010000014        30219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1067_length_30219_cov_6.194371, whole genome shotgun sequence.
ACCESSION   DAORUQ010000014 DAORUQ010000000
VERSION     DAORUQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 30219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30219
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_30219_cov_6.194371"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLW13_02560"
     CDS             complement(<1..488)
                     /locus_tag="PLW13_02560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HQY69278.1"
                     /translation="MQRIVLGSGSPYRKRLLQRLGLEFECLSPDIDETPASGEPPETL
                     VLRLSEAKARAVALRAPDALVIASDQVGATDGRLLGKPGTVERAQQQLRGISGREVRF
                     LTGLCVLDAPSGRLLSGVETCTVRLRELSDVAIRDYVRREQPLDCAGSFKVEGLGIAL
                     FRS"
     gene            478..1125
                     /locus_tag="PLW13_02565"
     CDS             478..1125
                     /locus_tag="PLW13_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HQY69279.1"
                     /translation="MRCMMARGKCWCGPNFAEKYGIFKHLFFDSAGCRPYDCAPMPTP
                     RVPRQADFRKLAVFGGQIEGTIALDDLPRIRAESMESGSGAEATLQLQFRIDDERYKV
                     IEGRVQASLSLQCQRCLGPMTLPVDAQVHLAMVWSEEEIPSLPQRFEGIVVGEGLSDL
                     YELVEEELLLAIPLAPRHPEGECALRQTVESDEVADDDERENPFAVLAKIKGSSS"
     gene            1206..1385
                     /gene="rpmF"
                     /locus_tag="PLW13_02570"
     CDS             1206..1385
                     /gene="rpmF"
                     /locus_tag="PLW13_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQY69280.1"
                     /translation="MAVGQARTTRSRRGMRRAHDALRGAALSTDATSGEVHRRHHVTP
                     DGFYRGKKVVESKDD"
     gene            1473..2420
                     /gene="fabD"
                     /locus_tag="PLW13_02575"
     CDS             1473..2420
                     /gene="fabD"
                     /locus_tag="PLW13_02575"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQY69281.1"
                     /translation="MAGLFACVFPGQGSQRVGMLSAIAEAEPLVRETFAEASEVLGYD
                     LWNLAQSGPQEALNLTERTQPLILCASVALWRVWRGRGGAMPAYMAGHSLGEFSALVC
                     AGALGFADAVRLVRNRGAYMQEAVPVGQGAMAAVLGLDDALIEQACLEAAGTEVVAAV
                     NFNSPGQVVIAGHAGAVERAIAACKSLGAKRAMPLPVSAPFHTTLMKPAGDRLAQDMA
                     TISLAIPAVPVIHNVHARPESDAGKIRALLVQQIASPVQWVASVRFMADAGVQHVVEV
                     GPGKVLGGLCKRIDAALECFYSEEPQDLDKALAAAGAPN"
     gene            2429..3172
                     /gene="fabG"
                     /locus_tag="PLW13_02580"
     CDS             2429..3172
                     /gene="fabG"
                     /locus_tag="PLW13_02580"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQY69282.1"
                     /translation="MNDMQRVCLVTGASRGIGAAIADALGAAGATVVGTATTEAGAAA
                     ISARFAANGIVGTGMALDVGSATAVDELIARVTEQFGAPLVLVNNAGITQDNIVLRMK
                     DEEWARVIDTNLNSIFRLSKAVLRGMTKARWGRIVNISSVVGSMGNAGQANYAASKAG
                     MEGFARALAREIGSRAITVNCVAPGFIDTDMTRELPEAQRQKLLEQIPAGRLGEASEI
                     AAVVAFLVSDAAAYITGETIHVNGGMYMR"
     gene            3311..3544
                     /gene="acpP"
                     /locus_tag="PLW13_02585"
     CDS             3311..3544
                     /gene="acpP"
                     /locus_tag="PLW13_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY69283.1"
                     /translation="MSSIEERVKKIVAEQLGVKEEDIQNSASFVEDLGADSLDTVELV
                     MALEEEFETEIPDEDAENITTVQHAIDYVESHL"
     gene            3752..4987
                     /gene="fabF"
                     /locus_tag="PLW13_02590"
     CDS             3752..4987
                     /gene="fabF"
                     /locus_tag="PLW13_02590"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQY69284.1"
                     /translation="MKRRVVVTGMGVVTPLGNTVSETWDGIVNGRSGIRPIEQFDVSA
                     YGTRFGGSIRGFDANAFLPPKEVRKFDDFILYGLGAAVQAMEDAGLVVEESFAPRVGA
                     AIGSGIGGIASIEKSHQIVLDSGPRRVSPFFVPGSITNMIAGYLSIRYGFSGPNLAIT
                     TACTTGTHNIGLGARLIAWGEVDAMIVGGSEMATTPVGLGGFSASRALSTRNDAPEAA
                     SRPWDRDRDGFVLSDGAGVLVLEELEFARRRGARIYAELIGFGMSGDAYHMTLPPEDG
                     RGAAAAMHNAIRDAGVTPQEIDYINAHGTSTPAGDLAESRAVQRVLGDAADRVAISST
                     KSMIGHLLGAAGAVEAVFSILAIRDGVAPPTINLDNPEEGCTLNYVPHVAQQRKIDIA
                     LSNSFGFGGTNGSLVFRRI"
     gene            4998..5843
                     /gene="pabC"
                     /locus_tag="PLW13_02595"
     CDS             4998..5843
                     /gene="pabC"
                     /locus_tag="PLW13_02595"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HQY69285.1"
                     /translation="MNQLVPLPRVWVNATETNLVPAEDRGLAFGDGLFETMRLHGARV
                     PLLERHLQRLQHGALRLRIALDIDELRAEISRFLSSSGTDEGILKLIVTRGDGGRGYR
                     VDAGNESRRILMQRPLARHPAQWWSEGVSIRHCDTRLGSNPALAGMKHLNRLEQVLAR
                     LEWDDAQIAEGLMCDQKGRIVEGVSTNLFLVSGGRLLTPVIDNCGVAGVMRGFILDVV
                     APELALHTEQVHCERALLGAAQEVFLCNAVIGVWPVRQLGTKRWPLGPVTRRVQAHVA
                     QFLAS"
     gene            5818..6879
                     /gene="mltG"
                     /locus_tag="PLW13_02600"
     CDS             5818..6879
                     /gene="mltG"
                     /locus_tag="PLW13_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQY69286.1"
                     /translation="MWRSSLRPERVLRAVELGLAACLLIVLVAWGTFTRWLDQPLALD
                     APVVVELPQGGNLTHFLDDLHESGLLDHPRWLRAQARIDGVADKVRAGEYRLEPGITP
                     RLLLEMLVAGRVVVYAVTLVEGMTVAQVLARLQEQPKLETALRGATADSLFGKLGVDA
                     GWSSAEGAFFPDTYQYSRGMSDADILLASHRALHEVLAEEWAGRDAGLPYRSPYEALI
                     MASLVEKETGVAEERQEIAGVFVRRLQKGMLLQTDPAVIYGLGESFDGNLTREHLRAP
                     GPYNTYLNAGLPPSPIALPGRAAIHAALHPAPGSALFFVARGDGSHEFSASLEEHNRA
                     VRKFQLQRRGTPYRSRPQD"
     gene            6917..7555
                     /gene="tmk"
                     /locus_tag="PLW13_02605"
     CDS             6917..7555
                     /gene="tmk"
                     /locus_tag="PLW13_02605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQY69287.1"
                     /translation="MARGRFISVEGGEGAGKSTNIEGVRARLHAAGLRVVVTREPGGT
                     PLAEEIRQLLLAPREEGVCEDTELLLVFAARMQHVERVIKPALARGDWVISDRFTDAT
                     IAYQGAGRRMGVERIQALRRLLLGDFAPDLTLLLDVPVDSGMQRLAGRGAPDRFEMEG
                     REFFDRVRAAYLQLAAAEPARFRVIDAAGPLPDVQAAVAQALEGFLAQDATP"
     gene            7552..8544
                     /gene="holB"
                     /locus_tag="PLW13_02610"
     CDS             7552..8544
                     /gene="holB"
                     /locus_tag="PLW13_02610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQY69288.1"
                     /translation="MTGSAPAPLPWQRNDWERLRARIVDQRLHHAVLLSGPAGIGKRL
                     LGELLARSILCRNPDRGQACGACRDCLLVTAGSHPDLLRIAPEEPGKQIRIEQIRGEF
                     TDFVMRTPSVAHAKAIVIDPADAMNTHTANCLLKSLEEPAPRTHMLLLSDAPARLLPT
                     IRSRCQQLRLMPPAPEQALQWLQSAGGGADAADLLGIAAGCPLGALRIREADAIGGFD
                     RAADMMRRAASPGAWIPELVSGCADLELRELLGWVFLFLADLSRALAHGSASGARIQR
                     AAAAIDELMPRVGAVHAARMLQRTLAARRDAASTANPNRQLLLEALLIDWQRGC"
     gene            8611..8961
                     /locus_tag="PLW13_02615"
     CDS             8611..8961
                     /locus_tag="PLW13_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY69289.1"
                     /translation="MTAPQGTRNGILSLTIKDKAVLYAAYMPFLQNGGLFIPTNKPYR
                     IGDEVFMLLNLMDEQEKIPVAGKVVWVTPKRAQGNRASGIGVQFNGQDDTASRKIETY
                     LAGALESERPTHTM"
     gene            8987..9769
                     /locus_tag="PLW13_02620"
     CDS             8987..9769
                     /locus_tag="PLW13_02620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQY69290.1"
                     /translation="MLIDSHCHLDYLTSPDVGMDIHAILQTAAAAGVGRMLTVAVDRE
                     NQLRVLAHAQAHEQVFASVGIHPSSCAAEPVSVAELLALAAQPKVVAIGETGLDYYHG
                     AEHAERQQQSFVTHLQAASRAGLPVIVHTRDAREDTLRLLAAHADPVHAGVMHCFTES
                     LEMARAAMEMNFMISFSGIITFRSAAALREVVRAVPLDRMLVETDSPYLAPVPHRGRT
                     NEPRHVLHVAEAVAAIKEIPLEEVAAATTANFYRLFPRAVVA"
     gene            9779..10852
                     /locus_tag="PLW13_02625"
     CDS             9779..10852
                     /locus_tag="PLW13_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HQY69291.1"
                     /translation="MRQLPFGLGLVEGFYGRQWRDEDRIACLRFIAGTGYRYYLYAPK
                     GDAVLRRRWPERWDAAEERRMRAIADCCAESGLDWGFGLSPLGLVEDPSPVNLRRLRD
                     KVRYLESFGARIQCILFDDMPRHVHALAASQLDIVAEVMSVSEAGHALVCPSYYSTDP
                     VLERVFGAMPADYWEELGAGLPREMGIFWTGDRVCAESHDAAGLGEIAARFARKPVLW
                     DNYPVNDGARGSNFLRIDAFRGRGSELAGLTQAHFVNPMNQCWLSRIALQSLALLYQQ
                     GPAYDADAAFAYCLRAQCEEALAAQLAADLDALQREGLSALSGARLAQMRARYAEFHS
                     PLAEEVRDWLHGGYAFDPACLTD"
     gene            10948..13098
                     /gene="fadB"
                     /locus_tag="PLW13_02630"
     CDS             10948..13098
                     /gene="fadB"
                     /locus_tag="PLW13_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HQY69292.1"
                     /translation="MHHQGKAITLRALESGIVELCFDRDADAVNKFDQLTLGELGEAL
                     GQIAADSGVRGVLITSAKPEYFIVGADIGEFGALFRQGEAALLENLARANAIFNALED
                     LAVPSVCAINGTALGGGLELCLAADARVMSRGARVGLPEVKLGINPGFGGTVRLPRVI
                     GVDNAVEWICGGSEYKAEVALKVGAVDAVVEPARLREAALDLLRQCMDGTLDYAARRR
                     EKTQPVQLNDIERMMAFMTGKSVVAAQAGPNMPAPVTAAKSMEKSVGLSRDEALEVEA
                     KFFARLALTNEADSLIGLFLNEQAVSKKAREWESAGRKVTHAAVLGAGTMGGGVAYQS
                     ASCGVPIVMKDIAEKGLEIGMREAGKLLSGQVSRGRMTPEAMAGVLRTIRPALDYSGF
                     GAVDFVVEAVVENENVKKRVLAECEAVLGSDAVLASNTSTISITRLAQALARPANFCG
                     MHFFNPVHRMPLVEVIRGEHSSDAAIATTVAYARQLKKTPIVVRDCPGFLVNRVLFPY
                     FGAFNALLRDGADFRQVDRVMQKFGWPMGPAHLLDVVGIDVAHHAAGVMAEGFPERMQ
                     IGFRTAVELMFEAGRFGQKTGAGFYRYEQDKRGKPVKTPDPEAQAMIAGISAAPREFE
                     EQQIVERMMVPMCNELVRCLDEDIVDSAGEADMALVMGIGFPVFRGGALRYMDHMGLA
                     EFCAAADRHAGIAAVYGVPESLRARARDGRRFYS"
     gene            13134..14309
                     /gene="fadA"
                     /locus_tag="PLW13_02635"
     CDS             13134..14309
                     /gene="fadA"
                     /locus_tag="PLW13_02635"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HQY69293.1"
                     /translation="MSFKDDDVVIIDCARTPMGRSKNGVFRNVRAEDLSADLVNALFA
                     RNPNVDPAAVEDVIWGCVNQTEEQGWNVARMFSLLTVVPHSAGAQTISRLCGSSMSAL
                     HIAAQSVMTGYGDVFLVGGVEHMGHLPMDKGIDPNPRMSKHVAKAAGMMGITAEGLAM
                     LHGITREQQDAFGVRSHRLAARAQDEGRWAREIIPVRGHDEDGFSKLITEDTTVRRDT
                     TLEGLGALAPAFDPRNGTVTAGTSSQIADGAAAMIVMSGRKARACGVQPRAKIRAMAV
                     AGVDPSIMGYGPVPATHKALARCGLAMKDIEAVELNEAFAAQALPVLKDLGLLDDMDT
                     RVNLHGGAIALGHPFGCSGARITGTLINVMEQRDLTLGLATMCIGMGQGIATVLERV"
     gene            14365..15315
                     /locus_tag="PLW13_02640"
     CDS             14365..15315
                     /locus_tag="PLW13_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQY69294.1"
                     /translation="MTGRRSPENWLLLASYGSVAVALILIVVKAAAWLYTGSASLLGS
                     LIDSFMDSMASLLSMAAVRYSLKPADEEHRFGHGKAESLAALAQSAFIMGSAVLLFLY
                     CLERILQAEEHVLEHTGIGIAVSVCAIVCTLGLLALQTHAIRLTGSTAISADRLHYKS
                     DLLMNGAVIISLLFAQRGVYLVDVVMGLFIAMIISRGAVQIGWEAFGLLMDKALSPEV
                     DQHIRELALSSPGVLGVHDLRTRRSGMRYIIQLHVELEDETSLLVAHGIADAVEFRLR
                     EAYPGADVIIHQDPHSAVAEEYAAAMAQGPLQLPAGLRPS"
     gene            15442..15526
                     /locus_tag="PLW13_02645"
     tRNA            15442..15526
                     /locus_tag="PLW13_02645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15476..15478,aa:Leu,seq:tag)
     gene            15866..17200
                     /gene="tig"
                     /locus_tag="PLW13_02650"
     CDS             15866..17200
                     /gene="tig"
                     /locus_tag="PLW13_02650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQY69295.1"
                     /translation="MQVSVEITSGLGRRLTIGVPAARVEAEVAARLEKAAKTVRLKGF
                     RQGKVPMRVVQQRFGAGVRQEVLGEVMSQSFYEAVRQEDLKPAGQPTIEPRTLASGRD
                     LEFIATFEVYPEIELIDFSRLSVERPVADVTEADIDRMIDVLRNQQASWSTVERAAAP
                     GDKVNIDYTGRRDGVAFDGGSATGSDLVLGSGRMIPGFESGIEGMVAGETRELKLRFP
                     EEYHAEDLRGAEVVFDVTLNSVAGKELPELGEEFFARFGVSEGGLDGFRAEVRANMER
                     ELKQALRNKVKARVMNALHERTDVEIPQALVRQEIETLRRQALRQFGGDAARFDASIL
                     PDEMFRAQAERRVALGLLLGEVIRRESLKADPARVRGVVEEMASTYEEPEEVINWYYG
                     NREQLASVEAVVLEDQVIDSILERATVTEVPCSYEEALRADPPKPATDESQS"
     gene            17267..17899
                     /gene="clpP"
                     /locus_tag="PLW13_02655"
     CDS             17267..17899
                     /gene="clpP"
                     /locus_tag="PLW13_02655"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQY69296.1"
                     /translation="MDQRGERSGSTINGLGLVPMVVEQTARGERSYDIYSRLLKERVI
                     FIVGPVEDYMANLVVAQMLFLESENPDKDIHLYINSPGGSVTAGLSIYDTMQFIKPDV
                     STMCIGQAASMGAFLLAGGTSGKRYALPNSRIMIHQPSGGAHGQASDIDIHAREILII
                     RERLNALLAKHTGQSVETVARDTERDNFMNPEKAREYGIIDEVLSHRPGK"
     gene            18019..19293
                     /gene="clpX"
                     /locus_tag="PLW13_02660"
     CDS             18019..19293
                     /gene="clpX"
                     /locus_tag="PLW13_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016978017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQY69297.1"
                     /translation="MTKESGGDKSKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLC
                     NDIIREEVQETSGDASRNKLPKPREICQILDEYVIGQRHAKKVLSVAVYNHYKRLREG
                     DRQRDEVELGKSNILLVGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDVE
                     NIIQKLLQKAEYDVEKAQMGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLEKIIRDRSEKSGIGFSAEVKG
                     KDEAKNVSQLLRELEPEDLVRYGLIPEFVGRLPVIATLEELDEPALIQILTEPKNSLT
                     RQFKKLFDMEGVELDFREDGLRAIAGRALARKTGARGLRSILESVLLETMYEIPSERD
                     VTKVVIDESVIKGDSAPLKVYESVQPKAAPGE"
     gene            19408..21828
                     /gene="lon"
                     /locus_tag="PLW13_02665"
     CDS             19408..21828
                     /gene="lon"
                     /locus_tag="PLW13_02665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQY69298.1"
                     /translation="MKSETAAWPVLPLLPLRDVVVFPHMVIPLFVGREKSIAALESAM
                     DDEKRILLVAQRSATEDDPGMKDVYRVGTIANVLQLLRLPDGTVKVLVEGSERAAIEG
                     VNAREGYLEARVRPLGEKVIDPSESDALVRTCMRMFEQYVNLGKKVPAEVLSALSGID
                     EPGRLADTIAAHMSLELAQKQQILEVDSTVARIEHLMGLMEGEIELFQVEKRIRGRVK
                     KQMEKSQREYYLNEQMKAIQKELGELEDSPSEVESLERRIRGAGMSKEAEAKALAELN
                     KLKMMSPMSAEASVVRSYLDWMVNVPWKKRSKVRNDLARAEQILEQDHYGLEEVKERI
                     LEYLAVQKRVGKAKGPVLCFVGPPGVGKTSLGESIARATNRTFVRMALGGVRDEAEIR
                     GHRRTYIGSLPGKILQKLTKAGVRNPLFLLDEVDKMGMDHRGDPASGLLEVLDPEQNH
                     SFNDHYLEVDYDLSDVMFICTSNSMNIPVPLLDRMEVIRISGYTEDEKVNIARRYLVP
                     KQMKNAGLGETEISFDDSALRDLVRYYTRESGVRSLERQIAKICRKRVKQLSLDKNPG
                     VAAVTAETLGEYSGVRKYRYGEALESNQVGQVTGLAWTSVGGELLTIEAVAVPGKGRQ
                     VKTGSLGDVMQESIQAAITVVRSRAKALGIRPDFHEKYDLHIHVPEGATPKDGPSAGV
                     GMCTALVSVLTGIAVRADVAMTGEITLRGQVLAIGGLKEKLLAAHRGGIKTVIIPEEN
                     ERDLKEIPDNIKSALEIRPVRWIDQVLDIALERTPEPVSDEEWKTLSEKAADEAARSA
                     DQGRINTH"
     gene            21948..22220
                     /locus_tag="PLW13_02670"
     CDS             21948..22220
                     /locus_tag="PLW13_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY69299.1"
                     /translation="MNKAELIDVIADKADISKASAARALDAALEGIVSALRGGDQVAL
                     VGFGTFSVKERAERQGRNPQTGATMLISASRNATFKPGKALKDAVS"
     gene            22260..24125
                     /locus_tag="PLW13_02675"
     CDS             22260..24125
                     /locus_tag="PLW13_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQY69300.1"
                     /translation="MLQNIRDKTQGTLAKIILALIVIPFAAFGIDAFFTGGVPEVARV
                     NGEAVTEPELAQNIELERRRLLSQMQDSVDAALLDEARLKGPVLEAMIDRTLLRQVAE
                     RSGFRVGEAMLNQLIIDNESFHENGVFSQARFQGLLASNGMSPAQFKDLMREEIMISQ
                     VTNGLEVSEFATDVELGNVARLTQQTLNVQYFSVPVEGAGTGVEVPADRIAAYFEENK
                     SEFLTEETLQVEYLELKLADLSQPVAEAEVRAEYERRAASFEGADERHAAHIMLSSLP
                     DEEAQARLAALRERALAGEDFATLARDNSEDAGSTASGGDLGFSSGDAFPAEFETALK
                     SLSPGEVSAPVKSESGWHLVKLLELRQQTAPSFEEMRAGIETELQRRAAEPLFVERSD
                     RLADLTFNSDDLAEASSALGLERKLSPEFGRRGGEGVFADARVIAAAFGEDVLANRQN
                     SERIELDDEHVVVLRLKEHKPARQQELAEVEVRIRDLLRESMVREKVRIEAEQLLAGL
                     DAGRSIEQIAKAAGYELKVVERYGRDAPGIPSELGEALFRTPRGADGSGRGTVVTAGG
                     DALVYRFDNFREGALEKLPAEQREMLGELLQRSRAREVQAHYQQRLRESSSVELL"
     gene            24132..24980
                     /locus_tag="PLW13_02680"
     CDS             24132..24980
                     /locus_tag="PLW13_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQY69301.1"
                     /translation="MSTPGPGPGAWGEAAPVLAAGATHLWLCDLDALRDSALLADYLS
                     LLDTDEMQRLQRLVFERDRHRFLVSHALLRYVLSLYAGRPAASWRFLKGTHGKPELAP
                     GQGARAPAFNLSHSGSLALLAVSDGAGLLGVDIEFHRPMRNFQSLATRNFAPREAASL
                     PGLDGAELAGEFYDLWTLKEAFVKACGAGLSQSLADFWFSFDRGAARLCFEAVPALEP
                     EPWRWAFWAYRLEGPYSAALARRAPSDTDATNGQPRCFATVPGRTWAAARAGCILQGS
                     SRITAN"
     gene            25083..26267
                     /locus_tag="PLW13_02685"
     CDS             25083..26267
                     /locus_tag="PLW13_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQY69302.1"
                     /translation="MFRIRTFNQISVKGLERFSRECYEVASEIGTPDAILLRSHKLAE
                     SEIGASVKAVARAGAGVNNIPIDACTARGIPVFNTPGANANAVKELVAAALLLASRGI
                     VEGISFVNSLSPDMPVAEMGALLEKEKKRFAGNELRGKTLGVVGLGAIGSKVARLGLG
                     LGMEVIGFDPALSVEAAWRLPSEVRRMENLGALFARADFISLHLPVLESTRHLVNAES
                     SAGFRKGACLLNFARDEIVDVEAVLQALDAGRLARYITDFPHPRLIGRQDVILMPHIG
                     ASTEEAEENCAMMAADQLRAFLEHGNIRNSVNFPGLELERTGGCRLAVTNRNIPGMLG
                     NVLSLLAGAGINVVDMLNRSRDNIAYNLIDVEGAPSEQVIAQLSAVEGVVNVRLIAPP
                     RC"
     gene            26299..26760
                     /locus_tag="PLW13_02690"
     CDS             26299..26760
                     /locus_tag="PLW13_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HQY69303.1"
                     /translation="MSRPPDFHIDRHGTWRYRGGVIEREAMVRLFAGMLCKRGEHYVL
                     RTPEQEVRVHVDDAPLLITDMEISPDSAGVRRIWMITDLGERFLLCREHPLFLREDRQ
                     RGEVRAYQRVRDGMTALVHRHVFYRMAEMAGVDSESGKTGVYSDGMFFALE"
     gene            complement(26815..28458)
                     /locus_tag="PLW13_02695"
     CDS             complement(26815..28458)
                     /locus_tag="PLW13_02695"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HQY69304.1"
                     /translation="MTRESMEYDVVIVGAGPAGLAAACRLRQLSSDISVCVVEKGSEV
                     GAHILSGAVFEPTALNELFPDWKALGAPLDTPVTEDEIYVLRNASAGIKVPGMLVPKT
                     MHNEGNYIISLGNLCRWLAGQAENLGVEIYPGFAAAEILFHEDGRVKGIATGDAGISA
                     SGEHKDSYTPGMELHAKYTLFAEGCRGHLGKQVIKRFGLDTGRGTQHYGIGIKELWEI
                     DPGKHRQGLVVHSAGWPLQESGALGGGFLYHLENNQVALGLITDLCYANPFVSPFEEF
                     QRYKQHPVIRQFLEGGKRLSYGARAITKGGPQALPKMSFPGGLLIGCDAGTLNFAKIK
                     GSHTAMKSGMVAAEAVHAALATRGQEGEEITGFTDAFMDSWAGRELQLQRNFGPAQHK
                     FGNVLGSAYAFVDINLFNGKLPWTLGDDLADHEQLKPAAQSRRIDYPKPDGVISFDRL
                     SSVFLSNTNHEEDQPCHLTLRDPDVPIRHNLALYDEPAQRYCPAGVYEIVESSEGGKR
                     LQINAQNCVHCKTCDIKDPTQNIVWVTPEGAGGPNYPNM"
     gene            28741..29490
                     /locus_tag="PLW13_02700"
     CDS             28741..29490
                     /locus_tag="PLW13_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQY69305.1"
                     /translation="MKVLVAVKRVVDYNVKVRAKGDGSGVDLNNVKMSINPFCEIAVE
                     EAVRLREKGVATEVIAVSLGDKACQEQIRTALALGADRGILVETAEELQPLAVAKLLQ
                     AIVAREGVDLVIVGKQSIDGDNNQTGQMLAALAGMSQGSFASELVIENGKARVTREID
                     GGLQTVELALPAVVTTDLRLNEPRYASLPNIMKAKKKQLDTLTPADLGVDVAPRMRVL
                     KVEPPPERKAGIKVGSVAELVDKLRNEAKVI"
     gene            29490..>30219
                     /locus_tag="PLW13_02705"
     CDS             29490..>30219
                     /locus_tag="PLW13_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQY69306.1"
                     /translation="MSTLVIAEHSNNELKSATHHTVTAAQALGGEVHVLVAGGGCQAA
                     AEAAARIPGVTKVLVADNAAYAHQLAENMSALIAELGTGYDYLLAPATSNGKNVMPRV
                     AALLDVAQISEITAVISGDTFQRPIYAGNVIATVQSTDARKVITVRTTGFDAAPAEGG
                     SAAIEAVSIAKDAGLSSFVGESLAKSDRPELTAARVVVSGGRGMQNGENFKLLEAVAD
                     KLNAAVGASRAAVDAGFVPNDYQVG"
BASE COUNT         5138 a         9459 c        10538 g         5084 t
ORIGIN      
        1 gaacggaaca gcgcgatgcc gagcccttcg accttgaaac tcccggcgca gtccaggggt
       61 tgttcgcgcc gcacgtaatc gcggattgcc acatcgctca gctcgcgcag gcgcacggtg
      121 caggtctcga cgccgctcag caggcgtccc gatggcgcgt cgagcacgca cagcccggtc
      181 agaaaacgca cttcccgccc cgagatgccg cgcagctgct gctgcgcacg ctccaccgtg
      241 ccgggcttgc cgagcagcct gccgtcggtc gcgccgacct ggtccgaggc aatgacgagt
      301 gcatccggag cgcgcagcgc cacggcgcgc gccttcgcct cgctcaggcg cagcaccagg
      361 gtctcgggtg gctcgccgct ggccggggtc tcgtcgatgt cgggggagag gcactcgaac
      421 tcgagcccga gccgctgcag caggcgcttg cgataggggg aaccggagcc gaggacgatg
      481 cgttgcatga tggcgcgggg caagtgttgg tgtggcccga actttgcaga aaaatacggt
      541 attttcaagc atttattttt tgacagtgcc ggatgccggc cctatgattg cgcgcctatg
      601 ccgacacccc gggttcccag acaggctgat ttccgcaagc tggcggtatt tggcggccag
      661 atcgagggta cgatcgcgct cgacgatttg ccgcgcatca gggccgaatc gatggagtcg
      721 ggctcgggcg cggaggcaac actgcaattg cagtttcgca tcgacgacga gcgttacaag
      781 gtcatcgaag ggcgggtgca ggcgagcctg agcctgcagt gtcagcgttg cctgggtccg
      841 atgacgctgc cggtggacgc ccaggtgcac ctggcgatgg tctggagtga agaagagatt
      901 ccgtcgttgc cgcagcgctt cgagggaatc gtggtagggg aaggcctgag cgacctctac
      961 gaactggtgg aagaagagtt gctgctggcg attccgctgg cgccgcgcca ccccgaaggg
     1021 gaatgcgcgt tgcgtcaaac ggtcgagagc gacgaagtcg cggacgacga cgagcgggaa
     1081 aacccgttcg ccgtgctcgc gaagatcaag gggagttcat cctgaggccc gacgggcgcg
     1141 gggcgggcat tcgatttcag tgaagcaccc ggtgggtgcg tgttcaaagt agtgaggagt
     1201 cagtcatggc agttggtcag gcaagaacca cccgttcccg tcgcggcatg cgtcgcgccc
     1261 acgatgcgct gcgcggcgcg gcattgtcga ccgatgccac ttccggtgaa gtgcatcgtc
     1321 gccaccacgt gaccccggac ggtttctacc ggggcaagaa agtggtggag agcaaggacg
     1381 actgaggcgc agcctgcacc tcgatcgacg gcgagcgacc ggccacggtg ctcgccgttt
     1441 tcttttgtgc aaccgcatga atggaggctg cgatggcggg gttgttcgct tgtgtctttc
     1501 cggggcaggg gtcccagcgc gtcggcatgc tgtcggcgat tgccgaagcg gagccgcttg
     1561 tccgtgaaac ctttgccgag gcctccgagg tcctcggcta tgacctgtgg aacctggcgc
     1621 agtccggacc gcaggaagcc ctcaatctca ccgagcgcac ccagccgctg atcctttgcg
     1681 ccagcgtggc gctgtggcgc gtgtggcgcg gccgcggtgg cgcgatgccc gcgtacatgg
     1741 ccgggcacag cctgggcgag ttcagcgcgc tggtatgcgc cggcgcactc ggcttcgccg
     1801 acgcggtgcg gctggtgcgc aaccgcggtg cctacatgca ggaagccgtg cccgtggggc
     1861 agggcgcgat ggcggcggtg ctgggtctcg atgacgcgct catcgagcaa gcctgcctcg
     1921 aggcggcggg gaccgaagtc gtcgcggcgg tcaacttcaa ttccccgggc caggtcgtga
     1981 tcgccggtca cgccggtgcc gtcgaacgcg cgatcgcggc ctgcaagagc ctgggcgcca
     2041 agcgcgcgat gccgctgccg gtgagcgcgc ctttccatac gaccctgatg aagcccgcag
     2101 gcgatcgcct ggcgcaggac atggcgacga tatccctcgc cattccggcc gtgccggtga
     2161 tccacaacgt gcacgcgcgt ccggagagcg atgccgggaa gattcgcgcc ctgctggtgc
     2221 agcagatcgc cagtcccgtg cagtgggtgg cttcggtgcg cttcatggcc gatgccggcg
     2281 tgcagcatgt tgtggaggtc ggccccggca aggtgctcgg cggcctgtgc aagcggatcg
     2341 acgccgcgct ggaatgcttc tacagcgagg agccgcagga tctcgacaag gcgctggcgg
     2401 cggccggcgc ccccaactga tatcgggagt gaatgacatg caacgagtct gtctggttac
     2461 cggcgcgagc cgcggcatcg gggcggcgat cgccgatgcg ctgggagcgg ccggcgccac
     2521 cgtggtgggc acggcgacca cggaagcggg tgctgccgcg atctccgcgc gctttgcggc
     2581 caatggcatc gtcggcaccg gcatggcgct cgacgtcggc tccgcaaccg cggtcgatga
     2641 actcatcgct cgcgtgacgg agcagttcgg cgccccgctg gtgctggtca acaacgcggg
     2701 catcacgcag gacaacatcg tgctgcggat gaaggacgag gaatgggccc gggtcatcga
     2761 caccaacctc aactcgatct tccgcctgag caaggcggtg ctgcgcggga tgaccaaggc
     2821 gcgctggggg cgcatcgtga acatcagttc ggtggtcggc tcgatgggta acgccggaca
     2881 ggcgaactac gcggccagca aggccgggat ggaaggcttc gcgcgcgcac tggcgcgcga
     2941 gatcggttcg cgtgcgatca ccgtgaactg cgtcgccccc ggcttcatcg acaccgacat
     3001 gacgcgcgag ctgcccgagg cgcagcgaca gaagctgctc gagcagatcc cggccgggcg
     3061 cttgggcgag gccagcgaga tcgccgccgt ggtggccttc ctggtctcgg atgcggcggc
     3121 ctatatcacc ggcgagacga ttcacgtgaa tggcggcatg tacatgcgct gaaacatttc
     3181 cccttttgtc aatattgcat gtaacggcgc gattcggggt agaatcgcgc cgcgtcaacg
     3241 agccattgcg ggaatgggga tttccgtggc caacggcaca ctcaaccact cacaggagca
     3301 acagatcaaa atgagcagca tcgaagaacg cgtcaagaaa atcgtcgcgg agcagctcgg
     3361 agtcaaggaa gaggacatcc agaattctgc ttccttcgtg gaagatctcg gcgccgattc
     3421 tctggacacc gtcgaactgg tgatggcgct cgaagaggaa ttcgaaaccg aaattcccga
     3481 cgaggatgcg gagaacatca ccacggtgca gcacgccatc gattacgtcg aatcacacct
     3541 ctgattcggt cccgccggga ggtcgcgatg gctgcgttgc ggcccgcgtc ctcccacagt
     3601 gcttgccagg caagctgacc catcggcgag cggcagccgc gcaggcccgc cgtagagggg
     3661 tcgtgataga ctttggacgc cgcggcgctg actgcgtttc gtcgcgggca tcccgatcct
     3721 acaaggcatc tcgcagacgg agtcctcggc gatgaaacga cgggtggtcg ttactggcat
     3781 gggggtcgtt accccgctcg gcaacacggt atccgaaacc tgggacggca tcgtcaacgg
     3841 gcgcagcggg atccggccta tcgagcaatt cgacgtatcc gcttacggca cgcgcttcgg
     3901 cggctcgatc cggggcttcg acgccaacgc cttcctgccg cctaaggaag tccgcaagtt
     3961 cgacgatttc atcctttatg gactcggcgc ggccgtgcag gccatggagg atgccggtct
     4021 cgtggtcgag gaatccttcg cgccccgcgt cggggccgcc atcggttcgg gcatcggcgg
     4081 catcgcctcg atcgagaaga gccaccagat cgtgctcgat tcgggtccgc ggcgcgtatc
     4141 gccattcttc gtcccgggct cgatcaccaa catgatcgcc ggctacctgt cgatccgcta
     4201 cgggttctcg gggccgaacc tcgcgatcac cacggcctgc accaccggca cccacaacat
     4261 cgggctgggg gcgcggctga tcgcgtgggg cgaggtcgat gcgatgatcg tcggcggctc
     4321 cgagatggcc accacgccgg tgggactcgg cggcttctcg gcctcgcgcg ccctgtcgac
     4381 tcgcaacgat gcgcccgaag cggcgagccg gccgtgggat cgtgatcgcg acggtttcgt
     4441 gctgagcgac ggcgccggcg tgctggtgct ggaggagctc gagttcgcgc gccgtcgcgg
     4501 tgcgcgcatc tacgccgagc tcatcggctt cggcatgagc ggcgacgcct accacatgac
     4561 cttgcctccg gaagacgggc gcggcgctgc ggcggccatg cacaacgcca tccgcgatgc
     4621 cggggtgacg ccgcaggaaa tcgattacat caatgcccac ggcacctcga cgcccgcggg
     4681 cgatctcgcc gagagtcgtg cggtgcagcg cgtgctgggc gacgcggcgg accgcgtcgc
     4741 gatcagttcg accaagtcca tgatcggtca cctgctcggc gctgctggcg cggtggaggc
     4801 ggtgttcagc atcctggcga tccgcgacgg ggtggcgccg ccgacgatca acctcgacaa
     4861 tcccgaggag ggctgcacgc tcaactacgt gccccacgtc gcccagcagc ggaaaataga
     4921 catcgcgctg tcgaactcct tcggtttcgg cgggaccaac ggctccctgg tctttcgcag
     4981 gatatgatcg ctgcatcatg aatcagctcg tgccgctgcc gcgggtctgg gtcaacgcga
     5041 cggaaacgaa cctggtcccg gccgaggacc gcggcctggc ctttggcgac ggcctgttcg
     5101 agacgatgcg cctgcatggc gcgcgcgtgc cgctgctgga gcgacacctg cagcggctgc
     5161 agcacggagc gctgcgcctg cgcatcgcgc tggacatcga cgaattgcgc gcggagatat
     5221 cccgtttcct gtcctcgtcc ggcaccgatg agggaatcct caagctgatc gtgacgcgcg
     5281 gcgacggggg gcgaggctat cgcgtcgatg ctggcaacga gtcacgtcgc atcctgatgc
     5341 agcgcccgtt ggcccggcat cccgcgcaat ggtggtcgga gggcgtcagc atccgtcatt
     5401 gcgacacgcg gctcgggagc aacccggcgc tggccggcat gaagcatctg aatcgcctgg
     5461 agcaggtgct cgcccgcctg gagtgggacg atgcgcagat cgccgaggga ctgatgtgcg
     5521 accagaaggg gcgcatcgtc gagggcgtct ccaccaacct gttcctcgtc agcggcgggc
     5581 ggctgctgac gccggtgatc gataactgcg gtgttgccgg ggtgatgcgg ggtttcatcc
     5641 tcgacgtcgt ggcacccgaa ctcgcgctgc ataccgagca ggtccattgc gagcgcgcac
     5701 tgctgggcgc ggcgcaggaa gtgttcctgt gcaacgcggt aatcggcgtc tggccggtgc
     5761 gccagctcgg gaccaagcgc tggccgctgg ggccggtgac acgcagggtg caggcgcatg
     5821 tggcgcagtt ccttgcgtcc tgagcgcgtg ctgcgcgcgg tcgaactggg cctcgcggct
     5881 tgcctgctga tcgtcctggt ggcctggggc acgttcactc gctggctgga ccaaccgctc
     5941 gcgctggacg cgcccgtggt ggtggagctc ccgcagggcg gcaacctgac gcatttcctc
     6001 gacgatctgc acgaaagcgg tctgctggat cacccgcgct ggctgcgggc acaggcgcgc
     6061 atcgacggtg tcgccgacaa ggtccgcgcc ggggagtacc gcctggagcc cgggatcacg
     6121 ccgcgcctgc tgctggagat gctggtggcg gggcgcgtgg tcgtctacgc cgtcacgctg
     6181 gtcgagggca tgaccgtggc gcaggtgctc gcccggctac aggagcagcc gaagctcgag
     6241 accgcgctgc gtggcgcgac ggcagactcc ctgttcggca agctcggtgt cgatgccggc
     6301 tggtctagcg cggagggcgc gttctttccc gatacctacc agtacagccg cggcatgagc
     6361 gatgcggata tcctgctcgc ctcgcaccgg gcgctgcatg aggtgctcgc cgaggaatgg
     6421 gctggtcgcg acgcgggcct gccgtaccgg agcccctacg aggcattgat catggcctcg
     6481 ctggtagaga aggaaaccgg tgtggccgag gagcggcagg agatagcggg cgtgttcgtt
     6541 cgccgtctgc agaaaggcat gctgctgcag acggatccgg ccgtgatcta cggccttggc
     6601 gagagtttcg acggcaacct gacgcgcgag cacctgcgcg cgccgggccc ctacaatacg
     6661 tacctgaatg ccggcttgcc accgtcgccg atcgcgctgc ccggacgcgc ggccatccac
     6721 gcggcactgc atccggcgcc cggatcggcg ctgtttttcg tcgcgcgcgg cgacggttcg
     6781 cacgagttct cggcaagcct cgaggaacac aaccgggccg tgcgcaagtt ccagttgcag
     6841 cgccgtggca cgccataccg ctcgcgaccc caggactgag aacggccgcg agcctcaatg
     6901 gagaaagatc ctcgacatgg ctagaggccg gttcatcagc gtcgaaggcg gcgaaggcgc
     6961 gggcaagagc accaacatcg agggggtccg cgcgcggctg cacgcggcgg ggctgcgcgt
     7021 ggtagtgacg cgcgagcccg gcggcacgcc gctcgctgaa gagatccgcc agctattgct
     7081 ggcgccgcgc gaggaagggg tgtgcgagga caccgagttg ctgctggtgt tcgccgcgcg
     7141 gatgcagcac gtggagcgcg tgatcaagcc ggcgctggcg cgcggcgact gggtgatcag
     7201 cgatcgcttc acggatgcga ccatcgccta ccagggggcg gggcggcgca tgggagtgga
     7261 gcggatccag gccctgcgcc ggctgttgct gggtgatttc gcgccggacc tcacgttgct
     7321 gctggacgtg ccggtggaca gcggcatgca acgccttgcg ggtcgcggtg cgccggaccg
     7381 cttcgagatg gaaggccggg aattcttcga ccgcgtgcgc gccgcctacc tgcaactggc
     7441 cgcggcggag cccgcgcggt ttcgggtgat cgacgccgcc gggccgctgc cggacgtcca
     7501 ggccgccgtc gcgcaggccc tcgagggctt cctcgcgcag gacgcgacgc cgtgaccggt
     7561 tccgcgccag cccctctgcc ctggcagcgc aacgactggg agcgactgcg cgcgcgcatc
     7621 gtcgatcaac gcctgcatca cgccgtgctg ctcagcgggc cggcgggtat cggcaagcgc
     7681 ctgctgggag agctgctggc gcgctcgatc ctgtgccgca acccggaccg cgggcaggct
     7741 tgcggagcgt gtcgcgactg cctgctggtc acggcgggct cgcacccgga tctgctgcgg
     7801 atcgcgcccg aggagccggg caagcagatc cgcatcgaac agatacgggg cgagttcacc
     7861 gacttcgtga tgcgcacgcc gagcgtggcc cacgccaagg cgatcgtgat cgatccggcg
     7921 gatgccatga acacgcacac cgccaactgc ctgctgaaga gcctggagga gccggcgccg
     7981 cgcacgcaca tgctgctgct gagcgatgcc ccggcgcgcc tgctgcccac gatccgctca
     8041 cgctgccagc agctcaggct catgccgccg gcgcccgagc aggcgctgca gtggttgcag
     8101 tcggccggtg gcggcgccga tgccgcggac ctgctcggta tcgcagcggg ttgtccgctg
     8161 ggcgcgctgc gcattcgcga ggcggatgcc atcgggggct tcgaccgcgc ggccgacatg
     8221 atgcgccgcg cggcatcacc cggagcctgg attcctgagc tggtgagcgg atgcgccgac
     8281 ctggagttgc gcgagctgct cggctgggtg ttcctgttcc tggccgatct gagtcgcgcg
     8341 ctggcgcacg gctcagcctc gggggctcgc atccagcggg ctgccgcggc catcgacgag
     8401 ctgatgcccc gtgtcggcgc cgtgcacgcc gcgcggatgc tgcagcgcac cctggcggcg
     8461 cgtcgcgatg cggcgtcgac ggccaatccg aaccgccagc tgctgctcga ggccttgctc
     8521 atcgattggc agcgaggctg ttgaggctgg caaaatccgg gttgagtcgc tactcttctg
     8581 caagtaccac cggagtgatt cccccagccc atgaccgcgc cgcaaggaac ccgcaacggc
     8641 atcctgtccc tgaccatcaa ggacaaggcg gtgctgtatg cggcgtacat gcccttcctg
     8701 cagaacggtg gcctgttcat cccgaccaac aagccctacc gcatcggcga cgaagtattc
     8761 atgctgctga acctgatgga cgagcaggag aagattccgg tggccggcaa ggtggtttgg
     8821 gtcacgccca agcgcgcaca gggcaaccgc gcctcgggca ttggcgtgca gttcaacggt
     8881 caggacgaca cggcaagccg caagatcgaa acctaccttg cgggcgccct ggaatccgaa
     8941 cgtcccacgc acacgatgtg agaagcggcc tgcgggccgt cggcgcatgc tcatagacag
     9001 ccattgtcat ctggactacc tcacgtcgcc cgacgtgggc atggacatcc acgccatcct
     9061 gcaaactgcc gcggccgccg gggtagggcg catgctcacg gtggcggtcg accgcgagaa
     9121 ccagttgcgc gtgctcgccc acgcgcaggc gcacgagcag gtctttgcat cggtcggcat
     9181 tcatccctcg tcctgtgccg cggagccggt cagcgtcgcc gagctgctcg cgctcgccgc
     9241 gcagcccaag gtggtcgcga tcggcgaaac cgggctcgat tactaccatg gcgcggagca
     9301 cgccgagcgc cagcagcaga gcttcgtcac gcatctgcag gcggcgtcgc gggcggggct
     9361 cccggtcatc gtgcatacgc gcgatgcgcg cgaggacacg ctgcggttgc tcgccgcaca
     9421 tgccgatccg gtgcacgccg gcgtgatgca ttgcttcacg gaaagcctgg agatggcccg
     9481 cgcggcgatg gagatgaact tcatgatctc gttctccggg atcatcacct tccgcagcgc
     9541 cgcggcgttg cgcgaggtcg ttcgcgccgt accgctcgat cgcatgctgg tggaaaccga
     9601 ctcgccctac ctcgcgccgg tgccgcatcg cgggcgcacc aacgagccgc gccacgtgct
     9661 gcacgtcgcc gaagcggtgg cggcgatcaa ggagattccg ctcgaggaag tggctgcggc
     9721 gaccacggcg aatttctacc ggttgttccc gcgcgcggtc gtggcctgag ggcgcgccgt
     9781 gaggcagttg ccgttcgggt tggggctggt ggaaggcttc tacggccggc aatggcgcga
     9841 cgaggaccgc atcgcctgcc tgcgcttcat cgcgggcacc ggctatcgct actaccttta
     9901 tgcacccaag ggcgatgccg tactgcgacg ccgctggccc gagcgctggg acgccgcgga
     9961 ggagcgacgc atgcgcgcga tcgccgactg ttgcgccgaa tccggactcg actgggggtt
    10021 cgggctgagt ccgctcgggc tggtcgagga tccctcgccc gtgaacctgc ggcgcctgcg
    10081 cgacaaggtg cgttacctgg agagcttcgg cgcacgcatc cagtgcatcc tcttcgatga
    10141 catgccgcgc cacgtccatg cgctggccgc ctcgcaactg gatatcgtcg ccgaggtgat
    10201 gtccgtcagc gaggccgggc atgcgctcgt ctgccccagc tattactcga ccgacccggt
    10261 gctggaacgc gtgttcggtg cgatgcccgc cgattactgg gaagagctcg gcgccgggct
    10321 gcctcgcgag atgggtatat tctggaccgg cgacagggtt tgcgccgaat cccacgatgc
    10381 ggcaggtctc ggcgagatcg cggcgcgttt cgcgcgcaag cccgtgctgt gggacaacta
    10441 ccccgtcaac gacggcgcca ggggatcgaa cttcctgcgc attgacgctt tccgcggccg
    10501 cgggagcgag ctcgccgggc tcacgcaggc gcatttcgtg aacccgatga accagtgctg
    10561 gctgtcgcgg atcgccttgc agtcgctggc gctgctctac cagcaggggc ccgcctacga
    10621 tgcagacgcc gctttcgcgt attgcctgcg cgcgcagtgc gaggaggcgc tggccgcgca
    10681 actcgccgcg gatctcgatg cgctgcagcg cgaggggctg agtgcgctgt ccggggcgcg
    10741 tctcgcgcag atgcgcgcgc gctatgccga atttcactcg ccgctggcgg aggaagtccg
    10801 cgactggctc cacggcggct acgccttcga tccggcctgc ctcaccgatt gagcgccgac
    10861 gctgtcttac gcagcaggta gtggccggcg aagccggcgc gctcctataa tcgccgccat
    10921 ccaattactg accgacgggg acattccatg catcaccagg gcaaggcgat cacgctgcga
    10981 gcgctggaat ccggtatcgt cgagctgtgc ttcgaccgcg acgcggatgc cgtcaacaag
    11041 ttcgaccagc tgactctcgg cgagctcggc gaggcgctgg ggcagatcgc cgcggacagt
    11101 ggcgtgcggg gcgtgctgat caccagcgcc aagcccgagt acttcatcgt cggcgccgat
    11161 atcggcgagt tcggcgcgct gttccgccag ggcgaggccg ccctgctcga gaacctggcc
    11221 cgggcgaacg ccatattcaa cgcactggaa gatcttgcgg taccgagcgt ctgcgccatc
    11281 aacggtaccg cgctgggcgg cgggctggag ctctgcctgg ccgcggatgc gcgcgtgatg
    11341 tcgcgcggcg cccgtgtcgg tctgcccgag gtcaagctcg ggatcaatcc cggcttcggc
    11401 ggcacggtgc gcctgccgcg cgtgatcggc gtcgacaacg cggtcgagtg gatctgcggc
    11461 ggcagcgagt acaaggccga ggtcgcgctg aaggtgggtg cggtcgatgc ggtggtcgag
    11521 ccggcgcgcc tgcgagaggc cgcgctggac ctgctgcgcc agtgcatgga tggcacgctg
    11581 gattacgcgg cacggcgtcg cgagaagacg caaccggtgc aactcaatga catcgaacgc
    11641 atgatggcct tcatgacggg caagagcgtg gtggccgcgc aggcggggcc gaacatgccg
    11701 gcgccggtga cggccgccaa gtcgatggag aaatccgtgg gcctgtcgcg cgacgaggcg
    11761 ctcgaggtgg aggcgaagtt cttcgcgcgg ctcgcgctca ccaacgaggc ggacagcctg
    11821 atcgggctgt tcctgaacga gcaggcggtt tcgaagaagg cgcgggagtg ggagtcggcc
    11881 ggacgcaagg tgacgcacgc ggcggttctc ggagcaggca ccatgggtgg cggtgtggcc
    11941 taccagtcgg cgagctgcgg cgtgccgatc gtgatgaagg acatcgccga gaagggactg
    12001 gagatcggca tgcgtgaggc cggcaaattg ctctcgggcc aggtcagccg cgggcgcatg
    12061 acgccagaag ccatggccgg cgtgctgcgc accatccgtc ccgcgctcga ttacagcggt
    12121 ttcggtgcag tggatttcgt ggtcgaggcg gtcgtcgaga acgagaacgt caagaagcgc
    12181 gtgctcgccg agtgtgaagc ggtgctgggc agcgatgccg tgctcgcgag caacacctcg
    12241 acgatctcga tcacgcgcct tgcacaggcc ctggcgcgcc cggcgaattt ctgcggcatg
    12301 cacttcttca acccggtgca ccgcatgccc ctggtcgagg tgatccgcgg cgagcacagt
    12361 tccgacgccg ccatcgccac cacggtggct tacgcgcgac agctcaagaa gacgccgatc
    12421 gtggtgcgcg attgcccggg cttcctggtc aaccgtgtgc tgttcccgta tttcggcgcg
    12481 ttcaacgcct tgttgcgcga tggcgcggac tttcgccagg tcgatcgcgt gatgcagaaa
    12541 ttcggctggc ccatggggcc ggcgcacctg ctggacgtgg tcggcatcga cgtggcgcat
    12601 cacgccgccg gcgtgatggc cgagggcttc cccgaacgca tgcagatcgg gttccgcacc
    12661 gcggtggaac tgatgttcga ggcggggcgc ttcgggcaga agaccggggc gggtttctat
    12721 cgttacgaac aggacaagcg tggcaagccg gtcaagacgc ccgaccccga agcgcaggcg
    12781 atgatcgccg gcatcagcgc cgcgccgcgc gagttcgagg agcagcagat cgtcgaacgc
    12841 atgatggtgc ccatgtgcaa cgaactcgtg cgctgcctcg atgaggacat cgtggattcg
    12901 gcgggcgagg ccgacatggc gctggtcatg ggcatcggct tccccgtgtt ccgcggcggt
    12961 gcgctgcgct acatggatca catgggcctg gcggagttct gtgccgccgc ggaccgccat
    13021 gcggggatcg cggcagtcta cggcgtgccg gagtcgttgc gcgcccgtgc gcgtgacggc
    13081 aggcgtttct attcctgata caccggtgcc tgcgtctgca ggggaggaac ggcgtgagtt
    13141 tcaaagacga cgatgtggtg attatcgact gcgcacggac cccgatgggg cgttcgaaga
    13201 acggcgtatt ccgcaatgtg cgcgcggaag acttgtccgc ggatctcgtg aacgcgttgt
    13261 tcgcgcgcaa tccgaacgtg gatcctgccg cggtggaaga cgtgatctgg ggctgcgtga
    13321 accagaccga ggagcagggc tggaacgtgg cacgcatgtt ctcgctgctc accgtggtac
    13381 cgcattcggc cggtgcgcag accatatcgc ggctctgcgg ctcctcgatg tcggcgctgc
    13441 acatcgccgc gcagtcggtc atgaccggct atggcgatgt gttcctggtc ggcggcgtgg
    13501 agcacatggg acacctgccg atggacaagg gcatcgatcc caacccccgc atgagcaagc
    13561 acgtcgccaa ggccgccggg atgatgggca tcaccgccga gggactggcc atgctgcacg
    13621 gcatcacgcg cgagcagcag gatgccttcg gcgtgcgctc gcaccgcctg gcggcccgag
    13681 cgcaggacga agggcgctgg gcgcgcgaga tcatcccggt gcgcggtcat gacgaggatg
    13741 gcttcagcaa gctgatcacc gaggacacca cggtgcgccg tgacaccacg ctcgaggggc
    13801 tgggtgcctt ggcgccggct ttcgacccgc gcaacggcac ggtcaccgcc ggaacctcgt
    13861 cgcagatcgc cgacggtgcg gcggcgatga tcgtgatgtc cggacgcaag gcgcgggcct
    13921 gtggcgtgca gccgcgcgcg aagatccggg cgatggcagt tgcgggggtg gacccttcga
    13981 tcatgggtta cggtccggtt ccggcgacac acaaggcgct ggcgcgctgc ggcctggcga
    14041 tgaaggacat cgaggcggtg gaactcaacg aggccttcgc cgcgcaggcc ctgcctgtgc
    14101 tcaaggacct cgggctgctc gacgacatgg acaccagggt caatctgcac ggaggcgcga
    14161 tcgcgctggg gcatcccttc ggttgctcgg gcgcgcggat taccgggacc ctcatcaacg
    14221 tgatggagca gcgcgacctc acgctgggac tggcgaccat gtgcatcggc atgggccagg
    14281 ggatcgcgac ggtgctggag cgggtttgat gccggggcag aggcaggggg gaatcgcgat
    14341 cccggagtcc ggggccgcgg ctgcgtgacc ggtcgtcgtt cccccgagaa ttggctgctg
    14401 ctggcgagtt acggttcggt cgcggtcgcc ctgatcctga tcgtcgtcaa ggcggcggcg
    14461 tggttgtata cggggtccgc cagcctgctc ggttcattga tcgattcctt catggattcc
    14521 atggcctcgc tgctgtcgat ggccgcggtg cgctattcgc tgaagccggc ggacgaggaa
    14581 catcgtttcg ggcacggcaa ggccgaatcg ctggcggcac tggcgcagtc ggcgttcatc
    14641 atgggttccg cggtgctgct gttcctgtat tgcctggagc gtattctgca agccgaggag
    14701 cacgtgctcg agcataccgg gatcggtatc gcggtcagtg tctgtgcgat cgtgtgcacg
    14761 ctcgggctgc tcgcgctgca gacacacgct atacgcctca ccggctcgac ggcgatcagc
    14821 gcagatcggc tgcactacaa gtcagacctg ctgatgaacg gggctgtcat catttcactg
    14881 ctgtttgccc agcggggtgt ctacctggtc gatgtggtca tggggctgtt catcgccatg
    14941 atcatctccc gcggcgccgt gcagatcggg tgggaggctt ttggtctgct gatggacaag
    15001 gcactgtcgc cggaggtcga tcagcacatc cgcgaactgg cgctgtccag tccgggcgtg
    15061 ctcggcgtgc acgatttgcg tacacggcgc tccgggatgc gctacatcat ccagttgcac
    15121 gtggaactgg aggacgagac gtcgctgctc gtcgcccacg gcatcgccga cgcggtggaa
    15181 tttcgcctgc gcgaggcgta tcccggtgcg gacgtgatca tccaccagga tccgcacagc
    15241 gcggtggcag aggagtatgc ggcggcaatg gcgcagggcc cgttgcagct gcctgcaggg
    15301 ttgcggccga gttgaattgc tgcgcagccg tgtggcaaac tgcggtcgcc tggaactccc
    15361 ctgactttgt caagaaatca tcggcataac aacaagttgc gcagatctcc tgattcagcg
    15421 gtcaaagccc ggcccggctg tgcgaaagtg gcggaactgg tagacgcgct ggatttaggt
    15481 tccagtgccg caaggcgtgc gggttcgagt cccgcctttc gcaccacaac gggaagggtt
    15541 gccgacggga aggcaccggg cagatgcgct ggccggtttc ccgattttgc cgactctcgg
    15601 atttcatgaa cccatgctag aatgcgcgcc cttgtggttg gcgtgccgct gccgaatggg
    15661 cgtgcgggtg cgtcgagcgc attagcggat caggctgttt ccgagcgata tccggttgtc
    15721 cattccctcc ggcgatgcgt ccggccttgg tggtcggtgc acgcgagtgg cgggccggaa
    15781 tggcgagttc cgtcacggat atgtggggcg ctgcgttgtg cgcgccttcg atgatcaatg
    15841 tcacagttct tgagcgaggt tgaaaatgca ggtttccgtt gaaattacgt cgggattggg
    15901 ccggcgcctg accattggcg tgccggcggc gcgcgttgag gcggaagtgg cggcacgcct
    15961 cgagaaggct gcgaagacgg tgcgcctgaa gggtttccgt cagggcaagg tgccgatgcg
    16021 ggttgtgcag cagcgcttcg gcgccggtgt tcgccaggag gtgctcggcg aggtgatgag
    16081 ccagtcgttc tacgaggccg tgcgccagga ggacctcaag cccgcgggcc agcccaccat
    16141 cgaaccccgt acgctcgcca gtggacgcga cctcgagttc atcgcgacat tcgaggttta
    16201 cccggaaatc gagctgatcg atttcagcag gctcagcgtc gagcgcccgg tggctgatgt
    16261 caccgaggcc gacatcgacc gcatgatcga cgtattgcgc aaccagcagg cgtcgtggag
    16321 cacggtggag cgcgcggccg cgccggggga caaggtcaac atcgactaca ccggacgccg
    16381 tgatggcgtc gcattcgacg gcggctcggc gacgggcagc gatctggttc tcgggtccgg
    16441 ccgtatgatc ccgggattcg agtccggtat cgaggggatg gttgccggtg aaacccgcga
    16501 gctgaagctg cgcttcccgg aggaatatca cgccgaggat ctgcgcggtg cggaagtggt
    16561 cttcgacgtc acgctgaaca gcgttgccgg gaaggagttg ccggagctgg gcgaggaatt
    16621 tttcgcgcgc ttcggtgtct ccgaaggtgg gctcgacgga ttccgtgccg aagtgcgcgc
    16681 caatatggag cgcgagctca agcaggcatt gcgcaacaag gtgaaggcgc gcgtgatgaa
    16741 tgcgctgcac gagcgcaccg acgtcgagat tccgcaggcg ctggtgcgcc aggaaatcga
    16801 gacgctgcgc cgccaggccc tgcgccagtt cggcggcgac gcggcacgat tcgatgccag
    16861 tatcctgccc gacgagatgt tccgcgcgca ggcagagcgt cgcgtggcgc ttggtttgct
    16921 tctgggcgaa gtgatccgtc gcgagagcct gaaggcagac ccggcgcgcg tacgcggtgt
    16981 ggtggaggag atggcttcca cctacgagga gccggaggaa gtgatcaact ggtactatgg
    17041 caatcgcgag cagctggcct ccgtcgaggc ggtggttctg gaggatcagg tgatcgactc
    17101 catcctggag cgcgcgacgg tcacggaggt tccgtgcagc tacgaggagg cgctgcgcgc
    17161 cgatccgccg aagccggcga ccgacgagtc gcagtcctga caaaccccgc ggaggggatg
    17221 cgtaagcgtt cctccgctta ttggccttga gcgaggagta catcgcatgg atcagcgtgg
    17281 cgagcgaagc ggtagcacca tcaacggtct cgggctcgtg cccatggtcg tggagcagac
    17341 tgcgcggggt gagcgctcct acgatattta ctcgcgtctg ctgaaggagc gcgtgatctt
    17401 catcgtcggt ccggtcgagg actacatggc caatctggtc gtggcccaga tgctgttcct
    17461 cgagtcggag aatcccgaca aggatattca cctttacatc aactctccgg gcggctcggt
    17521 caccgcaggg ctgtcgatct acgacacgat gcagttcatc aagccggacg tcagcacgat
    17581 gtgcatcggc caggccgcga gcatgggtgc cttcctcctg gcgggcggaa cgtcgggcaa
    17641 gcgctacgcg ctgccgaact cgcgcatcat gatccaccag cccagcggcg gcgcccacgg
    17701 ccaggcctcc gacatcgaca tccatgcgcg cgagatcctg atcattcgcg agcggctcaa
    17761 tgccctgttg gccaagcata cggggcagtc ggtggaaacc gtggcgcgcg acacggagcg
    17821 ggacaatttc atgaatcccg agaaggccag ggagtacggg atcatcgacg aggtgctgag
    17881 ccatcgtccg ggcaagtagg taccaatttg gtgccgtcct gctagactct cgaaagtgtt
    17941 ggggggggcg gacatgcggc ccgggcgcgc atggcacggc accccgggtg aactagggct
    18001 gattctggcg gagtggtgat gacgaaggaa agcggcggcg acaagagcaa actgctgtat
    18061 tgctcttttt gtggcaagag ccagcacgag gttcgcaagc tgatagcggg tccgtcggtg
    18121 ttcatctgcg acgagtgcgt cgatctgtgc aacgacatca tccgcgagga ggtccaggaa
    18181 acgtcgggcg acgcgtccag gaacaagttg cccaagcccc gcgagatctg ccagattctc
    18241 gatgagtacg tgatcggcca gcgccacgcg aagaaggtgc tgtcggtggc tgtttacaac
    18301 cactacaagc gtctgcgcga gggcgaccgg cagcgcgacg aggtcgagct gggcaagagc
    18361 aacatcctgc tggtcggccc gaccggcagc ggcaagacgc ttctcgccga gacgctcgcg
    18421 cgcctgctcg atgtgccctt cacgatcgcg gacgcgacca cgctcaccga ggcgggttac
    18481 gtcggcgagg acgtggagaa catcatccag aaactgctgc agaaagccga atatgatgtg
    18541 gaaaaggccc agatgggcat tgtctacatc gacgagatcg acaagatctc gcgcaagtcg
    18601 gaaaacccga gcatcacgcg tgacgtgtcg ggcgagggcg tgcagcaggc cctgttgaag
    18661 ctcatagaag gcacggtggc atcggtgccg ccgcagggcg gacgcaagca cccgcagcag
    18721 gagttcctgc aggttgacac ctcgaacatc ctcttcatct gcggcggcgc attcgcgggt
    18781 ctcgagaaga tcatccgcga ccgttcggag aagagcggca tcggtttctc cgccgaggtc
    18841 aagggcaagg acgaggccaa gaacgtgagc cagctgctgc gcgagctgga gcccgaggat
    18901 ctcgtgcgct acggcctgat ccccgagttc gtgggccgcc tgccggtcat cgcgacgctc
    18961 gaggaactcg acgaaccggc gctgatccag atccttaccg aaccgaagaa ctccctcacg
    19021 cgccagttca agaagctgtt cgacatggag ggagtggagc tggactttcg cgaggacggt
    19081 ttgcgcgcga tcgccgggcg agcgctggcg cgcaagaccg gtgcccgcgg gctgcgctcg
    19141 atcctcgaat ccgtgcttct cgaaacgatg tacgagattc cctcggaacg cgacgtgacc
    19201 aaggtcgtga tcgacgagtc cgtcatcaag ggcgactcgg cgccgctcaa agtctacgaa
    19261 tccgtccagc ccaaggctgc ccccggggag tgagttccct gcgtcgcgcg agtccggctc
    19321 gcgcacttcg agtagtatct tccggcggct tcccgtttcg tgagcggagt tgccggccca
    19381 ggcccgtgag gggcgaggag taacccaatg aaatccgaga ctgctgcgtg gccggtactg
    19441 ccgttgctgc ccctgcgcga tgtcgtggtg ttcccgcaca tggtcatccc gctgttcgtc
    19501 ggccgcgaaa agtcgatcgc ggcgctggaa tcggcgatgg acgacgagaa gcgcatcctg
    19561 ctggtcgcgc agcgcagcgc cacggaagac gacccgggca tgaaggatgt ctaccgtgtg
    19621 ggcacgatag ccaacgtgct gcagctcttg cggctgcccg acggcaccgt gaaggtgctg
    19681 gtcgaaggca gcgagcgtgc cgcgatcgag ggcgtcaacg cgcgcgaggg ctacctcgag
    19741 gccagggtgc gcccgctggg cgagaaggtg atcgatccat ccgaatcgga cgccctggtg
    19801 cgcacctgca tgcgcatgtt cgagcagtac gtgaatctcg gcaagaaagt gccggccgag
    19861 gtgctaagcg cgctcagcgg gatagacgag ccggggcggc tcgccgacac cattgccgcg
    19921 cacatgtcgc tggagctcgc gcagaagcag cagatcctcg aggtggattc gaccgtcgcg
    19981 cgcatagaac acctgatggg gctgatggaa ggcgagatcg aactgttcca ggtcgagaag
    20041 cgcatccgcg gtcgcgtcaa gaagcagatg gagaagagcc agcgcgagta ctacctgaat
    20101 gagcagatga aggccatcca gaaggagctg ggcgagctcg aggattcgcc gagcgaggtt
    20161 gagagcctcg agcggcgcat ccgcggcgca ggcatgtcca aggaggcgga ggccaaggcg
    20221 ctcgcggagc tcaacaagct gaagatgatg tcgccgatgt cggcggaagc ttccgtggtg
    20281 cgcagctacc tcgactggat ggtgaacgtg ccgtggaaga agcgcagcaa ggtgcgcaac
    20341 gatctcgcgc gcgccgagca gatcctggag caggatcatt acggactcga ggaggtcaag
    20401 gagcgaattc tcgagtacct cgcggtgcag aagcgcgtcg gcaaggccaa gggcccggtg
    20461 ctgtgcttcg tcggtccgcc gggcgtcggg aagacctcgc tgggcgagag catcgcgcgc
    20521 gcgaccaacc gtaccttcgt gcgcatggcg ctcggcggcg tgcgcgacga ggcggagatc
    20581 cgcggccacc gccgcaccta catcggctcg ctgccgggaa agatcctgca gaagctgaca
    20641 aaggcgggcg tgcgaaatcc gctcttcctg ctcgacgaag tcgacaagat gggcatggac
    20701 catcgtggcg atccggcctc ggggctgctc gaagtgctgg atccggagca gaatcacagc
    20761 ttcaacgacc attacctcga ggtggactac gacctgtcgg acgtgatgtt catctgcacc
    20821 tcgaactcga tgaacatccc tgtgcccctg ctcgaccgca tggaagtgat ccgtatctcg
    20881 ggctataccg aggacgagaa ggtcaatatc gcgcgccgtt acctggtgcc gaagcagatg
    20941 aagaacgctg gcctcggcga gacggaaatt tccttcgacg acagcgcgtt gcgcgacctg
    21001 gtgcgttact acacgcgcga gtcgggtgtg cgcagcctcg agcgccagat tgccaagatc
    21061 tgccgcaagc gggtcaagca gctctccctc gacaagaatc cgggcgtggc ggcggtgaca
    21121 gccgagacgc tcggtgaata ctccggcgtg cgcaaatacc gctatggcga agccctcgag
    21181 agcaatcagg tcggacaggt gaccggcctt gcgtggactt ccgtcggagg ggaactgctg
    21241 acaatcgagg ccgtcgcggt gccgggcaag ggccggcagg tgaagacggg atcgctcggc
    21301 gatgtgatgc aggagtcgat ccaggcggcg ataaccgtcg tgcgcagccg cgcgaaggca
    21361 ctcggtatcc ggcccgattt ccacgaaaaa tacgacctgc acatccacgt cccggaaggc
    21421 gctacgccca aggacgggcc cagcgctggc gtcggtatgt gcaccgcact cgtttcggtg
    21481 ctgaccggca tcgccgttcg cgccgacgtc gcgatgaccg gcgagatcac gctgcgcggc
    21541 caggtactgg caatcggtgg cctgaaggaa aagctgctcg cggcgcatcg cggcggcatc
    21601 aagaccgtga tcattcccga ggagaacgag cgcgacctga aggagattcc cgacaatatc
    21661 aagtcggcgc tcgagatccg cccggtgcgc tggatcgacc aggtgctgga catcgcgctc
    21721 gaacgcacgc ccgagccggt ctcggacgag gaatggaaga cgctcagcga gaaggccgcc
    21781 gacgaggcgg ctcgcagcgc cgaccagggc cggatcaata cgcactgaat cgagcagtgg
    21841 cttgaccgtg aagggcgagg gtggtgtaaa ctccccggcc cttcgcgcgc actcgggcgc
    21901 ggcaccacaa ccagtgttcc gaacacgaac ccatcaagga tatttctgtg aacaaagctg
    21961 aattgatcga cgtgattgcg gacaaggcgg acatttccaa ggcttcggcg gcgcgcgccc
    22021 tggacgcggc gctggaaggt atcgtgtccg cgctgcgcgg cggtgatcag gttgctctcg
    22081 tgggcttcgg cacgttttcg gtgaaggagc gcgcggagcg tcaggggcgc aatccgcaga
    22141 ccggcgcgac gatgctcatc tccgccagcc gcaacgccac gttcaagccc ggcaaggcac
    22201 tcaaggacgc ggtcagctaa ggtagtcagg ccgctcgcgc agaggattcc cacgtagcca
    22261 tgctgcagaa tatccgggac aagacccagg gcacgctcgc caagatcatc ctcgcgctga
    22321 tcgtgattcc gttcgcggcc ttcggcatcg atgccttttt cacgggtggt gtgcccgagg
    22381 tcgcgcgcgt caatggcgag gcggtgaccg agcccgagct cgcgcagaac atcgagctcg
    22441 aacgccgccg cttgctgtcg cagatgcagg acagcgtgga cgccgcgctg ctcgacgagg
    22501 cgcgcctgaa ggggccggtg ctcgaggcga tgatcgatcg cacgctgctg cgccaggtcg
    22561 ccgagcgctc gggctttcgc gtcggtgagg cgatgctcaa ccagctcatc atcgacaacg
    22621 agagcttcca cgagaacggg gtgttctcgc aggccaggtt ccagggactg ctcgccagca
    22681 acggcatgag tcccgcgcaa ttcaaggacc tgatgcgcga ggagatcatg atctcgcagg
    22741 tgacgaacgg tctcgaagtc agcgagtttg ccaccgatgt cgaactgggc aatgtcgccc
    22801 ggctcaccca gcagacactg aacgtgcagt acttcagcgt cccggtcgag ggtgccggga
    22861 ctggtgtcga ggttccggcg gatcggattg ccgcttactt cgaggaaaac aagtccgaat
    22921 tcctcaccga ggagaccctg caggtcgagt acctcgagct gaaactcgcc gacctgtccc
    22981 agccggttgc cgaggcggag gtgcgcgcgg aatacgaacg ccgcgcggcg agtttcgagg
    23041 gagcggacga gcgccacgcc gcgcacatca tgctctcctc gctgcccgat gaggaggcgc
    23101 aggccaggct ggccgcgctg cgcgaacgcg ccctcgccgg cgaggacttc gcaaccctgg
    23161 cacgtgacaa ttccgaagat gccggttcca ccgcgagcgg cggggacctt gggttctcca
    23221 gcggcgatgc attccccgcc gagttcgaaa cggcgctgaa gtcgctctcg cccggcgaag
    23281 tgtcggcccc ggtcaagagc gaatcggggt ggcatctggt caaactgctc gaactgcgcc
    23341 agcagaccgc gccctccttc gaggagatgc gcgcgggcat agagaccgag ttgcagcgtc
    23401 gcgcggcgga gcccttgttc gtcgagcgct ccgaccggct tgccgacctg acattcaatt
    23461 ccgatgacct ggcggaagcc tccagcgcgc tcgggctgga gcgcaagctg agtcctgagt
    23521 tcggtcgtcg cggcggcgag ggggtgttcg cggatgctcg cgtgatcgcg gccgcctttg
    23581 gcgaggacgt gctcgcgaac cggcagaaca gcgagcgcat cgagctcgat gacgaacacg
    23641 tggtggtgct gcggttgaag gagcacaaac ccgcacggca gcaggaactg gccgaggtcg
    23701 aggtacgtat ccgcgatctg ctgagggagt ccatggtgcg cgaaaaggtg cgtatcgagg
    23761 ccgagcagct gctggcgggg ctcgatgcgg ggcgcagcat cgagcagatc gcgaaggccg
    23821 ccggctacga actcaaagtc gtcgagcgtt acggtcgcga tgcgcccggg atcccctccg
    23881 agctcggcga agcgctgttc aggacgccgc gcggcgccga cggttccggt cgcggcacgg
    23941 tggtgaccgc cggcggtgac gcgctggtct accggttcga caatttccgc gaaggtgcgc
    24001 tcgagaagct gccggcggaa cagcgcgaga tgctgggcga gctgctgcag cgctcgcgtg
    24061 cgcgcgaggt ccaggcgcat taccagcagc gcctgcgcga gtcgtcgtcc gtcgagttgc
    24121 tgtagcaacc ggtgtcgact cccgggcctg gaccgggcgc ctggggcgag gcggcgccgg
    24181 ttctcgcggc cggcgccacg cacctctggt tgtgcgatct cgacgcgctg cgtgattccg
    24241 cgctgctcgc agactacctc tcgctgctcg ataccgacga gatgcagcga cttcagcgcc
    24301 ttgtgttcga gcgcgaccgt caccggttcc tcgtaagcca cgcgctgctg cgctacgtgc
    24361 tttctctcta cgccgggcgg ccggccgcca gctggcgatt cctgaagggt acgcacggca
    24421 agcccgaact cgcgcctggc cagggcgcgc gtgcccccgc cttcaatctt tcgcacagcg
    24481 ggtcactggc gctgctcgcg gtcagcgacg gtgcggggct gctcggcgtc gatatcgagt
    24541 tccatcgacc gatgcgcaac tttcaatccc tcgcgacgcg caacttcgcc ccgcgcgagg
    24601 cggcgagtct gcccggtctc gacggcgcgg agctcgccgg cgagttctac gatctgtgga
    24661 cgctcaagga ggccttcgtg aaggcctgcg gcgccggact cagccagtcg ctggccgatt
    24721 tctggttcag tttcgatcgc ggggccgcac gcctgtgctt cgaggcggtg ccggcgctgg
    24781 agccggagcc ctggcgctgg gcattctggg cttaccgcct ggagggcccg tatagcgcag
    24841 cgctggcgcg gcgcgccccc agcgacaccg acgccacgaa cggccagcca cgctgcttcg
    24901 cgaccgtgcc gggccgtacc tgggcggccg cgcgcgccgg ctgcatcctg caaggcagca
    24961 gtcggattac cgccaattga gacacggaat cgcgcacccg ctgcgctaga atacgcgccc
    25021 ttcgccccgc tggacgggac gcccggccgc cggatgcagc catcaacagg gagagatcgg
    25081 caatgttcag gattcgcacc ttcaaccaga tttcggtcaa gggcctcgag cgtttcagtc
    25141 gcgagtgcta cgaggtggcc agcgaaatcg ggacgccaga cgccattctg ctgcgcagcc
    25201 acaagctcgc cgaatccgag ataggagcgt cggtcaaggc agtcgcccgt gccggtgccg
    25261 gggttaacaa tattccgatc gatgcctgca ccgcgcgcgg tatcccggtg ttcaatacgc
    25321 ctggcgccaa cgccaatgcg gtcaaggaac ttgtggcggc cgcgctgttg ctcgcctcgc
    25381 gcggcatcgt cgaggggatt tccttcgtca actcgctgtc gccggacatg ccggtggccg
    25441 agatgggtgc gctgctagag aaggagaaga agcgcttcgc gggtaacgaa ctgcgcggca
    25501 agacgctcgg cgtcgtgggt ctcggcgcca tcggcagcaa ggtggcgcga ctcggcctcg
    25561 gcctcggcat ggaagtgatc ggtttcgatc cggcgctgtc ggtggaggcc gcgtggcgac
    25621 tgccgagcga agtgcgccgg atggaaaatc tcggtgcgct tttcgcgcgc gccgatttca
    25681 tcagcctgca ccttccggtg ctcgagtcga cgcgccacct ggtcaacgcc gaaagcagcg
    25741 ccggttttcg caagggcgcc tgcctgctga atttcgcgcg cgacgagatc gtcgacgtcg
    25801 aggcggtgct gcaggcactc gatgccgggc gcctggcacg ctacatcacc gacttcccgc
    25861 atccgcgtct gatcggccgc caggatgtga tcctgatgcc gcacatcggc gccagcaccg
    25921 aggaggccga ggagaactgc gcgatgatgg ccgcagacca gctgcgcgcg tttctcgagc
    25981 acggcaacat ccgcaattcc gtcaacttcc ccgggctcga actcgaacgc accggcggtt
    26041 gccgccttgc ggtgacgaac cgcaacattc ccggcatgct gggcaatgtc ctctcgctcc
    26101 tggccggtgc gggcatcaac gtcgtcgaca tgctcaatcg cagccgcgac aacattgcct
    26161 acaatctcat cgacgtcgag ggtgcgccct cggagcaggt gatcgcgcag ctctcggccg
    26221 tcgaaggggt cgtgaacgtg cggctgatcg caccgccgcg ctgctgatag tgccggtgcg
    26281 ggccgcagcg ccggcgcgat gagtcggcca cccgatttcc acatcgatcg ccacggcacc
    26341 tggcgctatc gcggcggcgt gatcgagcgc gaggcgatgg tgcgcctgtt tgcgggaatg
    26401 ctgtgcaagc gtggtgaaca ctacgtgctg cgcacgccgg aacaggaggt gcgcgtgcac
    26461 gtcgacgacg cgcccttgct gatcaccgac atggagatct cgccggatag cgccggggtg
    26521 cggcgcatct ggatgatcac tgacctgggc gaacggttcc tgctgtgccg cgagcacccg
    26581 ctgttcctgc gcgaggatcg gcaacgaggc gaagtgcgcg cgtatcagcg ggtacgcgac
    26641 ggcatgacgg cgctggtgca ccgccacgtg ttctaccgta tggccgagat ggcaggcgtc
    26701 gacagcgaga gcgggaagac gggggtgtac agcgacggga tgttcttcgc gctggagtga
    26761 agcgctgatt tgccgcagac ccggcgtact gcgcgccggg cccgcggcgg gttctcacat
    26821 attcgggtag ttgggtccgc cggcgccttc cggtgtgacc cagacgatgt tctgcgtcgg
    26881 gtccttgata tcgcaggtct tgcagtgcac gcagttctgc gcgttgatct gcaggcgctt
    26941 gccgccctcg ctgctttcca cgatctcgta cacgccggca ggacagtagc gctgcgcagg
    27001 ctcgtcgtag agcgccaggt tgtggcggat cggcacgtcg ggatcgcgca gcgtcaggtg
    27061 gcagggctgg tcttcctcgt ggttcgtgtt cgagaggaac accgagctga gccggtcgaa
    27121 gctgatcacc ccgtcgggct tcgggtaatc gatgcgacgg ctctgcgcgg ccggcttcag
    27181 ctgctcgtgg tcggcgaggt cgtcgccgag cgtccagggc agcttgccgt tgaagaggtt
    27241 gatgtccacg aaggcatatg ccgaaccgag cacgttgccg aacttgtgct gcgcaggacc
    27301 gaagttgcgc tgcaactgca gttcacggcc ggcccacgag tccatgaagg catcggtgaa
    27361 gccggtgatt tcctcaccct cctggccgcg ggtggcgagc gcggcatgca ctgcctcggc
    27421 agcgaccatg ccgctcttca tcgcggtgtg cgagcccttg atcttggcga agttcagcgt
    27481 gccggcatcg cagccgatca gcagtccgcc ggggaaactc atcttcggca gcgcctgtgg
    27541 gccgcccttg gtgatggcgc gcgcgccgta gctcagacgc ttgccgccct cgaggaactg
    27601 gcggatcacc ggatgctgct tgtagcgctg gaattcctcg aacgggctga cgaagggatt
    27661 ggcgtagcac agatcggtga tcaacccgag cgcgacctgg ttgttctcga ggtgatagag
    27721 gaatccgccg ccgagcgcgc cgctctcctg gagcggccag ccggcgctgt ggaccaccag
    27781 cccctgacgg tgcttcccgg gatcgatttc ccacagttcc ttgatgccga tgccgtaatg
    27841 ctgcgtgccg cgaccggtgt cgaggccaaa gcgcttgatc acctgcttgc cgaggtgacc
    27901 gcggcagcct tccgcgaaca gggtgtattt tgcatgcagt tccatgccgg gcgtgtagct
    27961 gtccttgtgc tcgcccgagg ccgaaatgcc ggcgtcgccg gtggcgatgc ccttcacgcg
    28021 accgtcttcg tggaacagga tttccgcggc ggcgaagccg gggtagatct ccacaccgag
    28081 gttttcggcc tgcccggcga gccagcggca gaggttgccc aggctgatga tgtagttgcc
    28141 ctcgttgtgc atcgtcttgg gcaccagcat gcccggcacc ttgatgcccg cgctcgcgtt
    28201 gcgcagcacg tagatctcgt cctcggttac cggcgtgtcg agcggtgcac cgagcgcctt
    28261 ccagtccggg aacagttcgt tcaatgctgt cggttcgaac actgcgccgg aaaggatgtg
    28321 cgctccgacc tcggatcctt tctcgaccac gcagacgctg atgtcgctgg agagctggcg
    28381 cagccggcag gccgcagcca ggccggcggg gcctgcaccc acgatgacga cgtcgtactc
    28441 catggattct ctagtcacgg cgctgctcca aaaaacgtat ggactacaaa gacttgcaga
    28501 tgcgcgggga cgatacacga ggagcgcggg gattgcaaaa ggaaagcatg ccgcgccgcc
    28561 gtgatgccgg tgtctggttg ccgcgaggcg tgagcggatg tgccgtgccc cccggtatcg
    28621 ttcctgctat tgacctgccc gctgcatgtc ccgaaaatac gcggcctttg cagctggccg
    28681 gccgcgctcc cgggcgtgcc aagtccccca ccaacctttc ccgagccaac cggagagtcc
    28741 atgaaagttc tcgtagcggt aaaacgcgta gtggattaca acgtcaaggt ccgcgccaag
    28801 ggcgacggat ccggcgtgga tctcaacaac gtgaagatgt cgataaaccc cttctgcgag
    28861 atcgcggtcg aggaagccgt gcgtctcagg gagaagggtg ttgccaccga ggtgatcgcc
    28921 gtttcgctgg gcgacaaggc ctgccaggaa cagatccgca ccgcgctcgc gctcggcgcc
    28981 gatcgcggca tcctggtcga gaccgccgag gagttgcagc cgttggctgt cgcaaagctg
    29041 ttgcaggcca tcgttgccag ggagggcgtg gacctggtca tcgtcggcaa gcagtcgatc
    29101 gatggcgaca acaaccagac cgggcagatg ctggcggcgc tggcgggcat gtcgcagggc
    29161 tccttcgcct cggaactggt gatcgagaac ggcaaggcgc gcgtaacccg cgaaatcgac
    29221 ggcggcctgc agacggtcga actggcgctc cccgccgtcg tgactacgga cctgcgcctg
    29281 aacgagccgc gctatgcgtc gctgccgaac atcatgaagg cgaagaagaa gcagctggac
    29341 acgctgacgc cggccgatct gggtgtggac gtcgcgccgc gcatgcgcgt attgaaggtc
    29401 gagccgccgc ccgagcgcaa ggccggcatc aaggtcggtt cggttgccga actggtggat
    29461 aaactgcgta acgaggcgaa ggtgatctga tgagcactct ggtaattgct gagcacagca
    29521 acaacgaact gaagtccgca acgcatcaca ccgtcacggc cgcgcaggca ctgggtggtg
    29581 aagtccatgt gctggtcgcc ggtggcggct gccaggcagc cgccgaggcg gccgcgcgca
    29641 ttcccggcgt gaccaaggtg ctggttgccg acaacgcggc ctatgcgcac cagcttgccg
    29701 agaacatgag cgcgctgatc gccgaactcg gcacgggtta cgactacctg ctcgcgcccg
    29761 cgaccagcaa cggcaagaac gtgatgccgc gcgtggccgc gctgctcgac gtggcgcaga
    29821 tctccgagat caccgccgtg atcagcggcg acacattcca gcgtccgatc tacgccggca
    29881 acgtcatcgc gacggtgcag tcgaccgatg cgcgcaaggt catcaccgtg cgtaccaccg
    29941 gtttcgatgc ggctcccgcc gagggcggca gcgctgcgat cgaggcggtg tcgatcgcga
    30001 aggacgcggg gctgtcctcg tttgtcggcg agtcgctggc caagtcggat cgtccggaac
    30061 tcaccgccgc gcgcgtggtg gtctcgggtg gccgcggcat gcagaacggc gagaacttca
    30121 agctgctcga ggcggtggcc gacaagctga atgcggcagt gggcgcctcg cgcgccgcgg
    30181 tcgatgccgg tttcgtgccc aacgattacc aggtcggtc
//
LOCUS       DAORUQ010000015        29864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1091_length_29864_cov_6.536952, whole genome shotgun sequence.
ACCESSION   DAORUQ010000015 DAORUQ010000000
VERSION     DAORUQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29864
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1091_length_29864_cov_6.536952"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLW13_02710"
     CDS             <1..450
                     /locus_tag="PLW13_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69307.1"
                     /translation="GARQLAIIDCDYHYGDGTHDILLRLGLYDHVRHWSLAARSGSER
                     QALARLEAALAENLPGSELLIYQAGADSHEDDPLGGVFTTAGYRERDRMVFTAAARAG
                     VPVAWNLAGGYQEPLGKVLELHAITMGEALVAEGVGANTALHAGQEQ"
     gene            921..3689
                     /locus_tag="PLW13_02715"
     CDS             921..3689
                     /locus_tag="PLW13_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HQY69308.1"
                     /translation="MHTDSVLNLPDESRMLDVVRRGLESADDVRIVVSFTRCSGLGLL
                     IDSLRQVAERGGKIRILTSTYQSVTQPEALDVLLQLPRVETRVQMGSVGLHAKFWWFY
                     GDRGAECWAGSSNLSKGGLATNIEWNVRRIDSAVIQETRHQFDRLWGRADVRELDDQL
                     IRLYRKSYLENAAANMVRPARVEYHLDPVNPNEAQVEALTRLAELRGRGERRAAVIAA
                     TGVGKTYLAALDVLQSGSRTALYISHRLEHLHQARRAFSRVFGDSLKLGVVGGGLDEA
                     DADIVFATISSIATRPNLAGRKFDYLVVDEFHHVEAPSYDVLRALRDQAFLLGITATP
                     ERQDGHDVLAWCDWNVAYEVRLPEAIDRGWLLPFHYFGIADETVDFLKIPWRRLNEVE
                     DALSVDARAEHVLHHALERGFDGPKRATVGFCAGRKHAKFMAAAFVRRGHAAVAVLGD
                     QTVERREEVYRQLADIDDPLQWVFVSDVLNEGVDIPAVNSVLFLRPTESATLFLQQLG
                     RGLRLSPGTEVLTVLDFVGHHRSAWLTLNALNAPSGGGRRTEIADGVTIKPPAACEVV
                     LQPRTREILSKVARFTTRREACDEAYIRLRAELGRVPMPVDLWNRADVPELRDFRAAY
                     RGWLDCQIANGDTPEWVIGLAQDHSVRDFLRIVESDWQAQRVSPYALLWGLCASPDFP
                     EVGYEQFFDRWMQWKSERSALDSSRTWSTLRRKLGSALEGDRIAGSIREALSEHLLAE
                     VEGRLLYTVNNDHRQRHGGVLRSPSDLNLFAMYDRPEIVRHFGVHYDPAKHNTGMLWF
                     GGDGVIITKLDTSTAIARHQYRNRLIDDRTFSWTSQNRMGTGNEAGLKVVEHEMRGLR
                     LYLFVQPRSHAPACYLGLVRVAKRADAEGSGPMQVRFSLSRSVPADVLKELLESDSEA
                     LVVGGADD"
     gene            3762..4163
                     /locus_tag="PLW13_02720"
     CDS             3762..4163
                     /locus_tag="PLW13_02720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQY69309.1"
                     /translation="MNRCPFCALSGDRIVASDGLSVVFRDGFPISLGHTLIIPRRHIG
                     SFFEATPDERASLLHLLDQAQQALASEFHPDGWNIGINDGPAAGQTVPHLHIHLIPRY
                     TGDSADPRGGVRWVIPGKANYWDSGSGNGNP"
     gene            4150..5274
                     /locus_tag="PLW13_02725"
     CDS             4150..5274
                     /locus_tag="PLW13_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HQY69310.1"
                     /translation="MEIRDSGGGAAYSVAGVIRPPPPEERLAFLRTLQRVLKEGDFTA
                     TYKFALLQALLDIAVESRIRNDAELPVSYRQLAERFIEYYWGHTEPYRPGDESGGVLA
                     QSTGRQAAVLTLLAAARGQSGSRTLAQLRREPRYPGLLSRVARVIREQPVRYIQNLNG
                     GHIEFLFAPAADGKGIVLKRGVAWCLQEFYDLLQSQVRAGWVQQVRSMSRNASLIGGH
                     KDLEEFLFGQQRMALVRIRERLVDLEGPTCFYCEKLIAHGIEVDHFVPWSRYPHDRGH
                     NFVLAHAACNHSKRDTLAGRDHLERWLARIDDKAGPISDALSDVLLCDAASSAKVAQW
                     AYLHEIHAAGMFWAGFAGRSPVYQPVDDALRSLVVSGFPM"
     gene            complement(5382..5882)
                     /locus_tag="PLW13_02730"
     CDS             complement(5382..5882)
                     /locus_tag="PLW13_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69311.1"
                     /translation="MSNETLHQRRDVIAGLAGLGAALAGAAAFAQPDASVAHAGHDMK
                     DAAPAPVLSAAHKRLIDATAACLKAGNFCMARCTDHLATGAPLMADCQRSVMNMLPVV
                     QATFAVAGHANAKPADLKALAKTCAQFCRSCAEACKPHSAHHEECKACEDACLDCADA
                     CDAVAA"
     gene            complement(6005..6520)
                     /locus_tag="PLW13_02735"
     CDS             complement(6005..6520)
                     /locus_tag="PLW13_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69312.1"
                     /translation="MRPLPHDPRTTEWIATSHKPALAEACVRYALAFNTLERNWLAGL
                     LADDVQYGSQQVFDIVHGRTPVCEHLGRKIEALRALPGREPRFELARSPTFGECLAGF
                     ERLEAAERNWLARPRLTAVLTVNGAGQVASVMIVTVAPSPAHVQRSGIYPGIGSGTVH
                     EIAQRDTGTGR"
     gene            6725..7117
                     /locus_tag="PLW13_02740"
     CDS             6725..7117
                     /locus_tag="PLW13_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQY69313.1"
                     /translation="MTEPRYSIGELAELGRVSRRTVHFYVQQGLIDPPEGRGRGSYYT
                     DRHLEQLQRVLALQREGLPLRRIQGMSESGQLRAAEAVLARQLVLRLAVAPGVTLEIE
                     AGSGGEIPSAEQLRELARRCAEVLGNDS"
     gene            7182..9671
                     /locus_tag="PLW13_02745"
     CDS             7182..9671
                     /locus_tag="PLW13_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HQY69314.1"
                     /translation="MGTMEQAAGGMFVRSSQAPVPLTGVSIEVTGSGSAAEVCVRQRF
                     VNVERVPIEAVYSFPLEEGSAVCELVMETGGKRLLGRIEEREKAFEQYDEALAAGHGA
                     FLADQDRPNIFTISAGNLLPGQEATVCLRYATELEQHGGELRIMLPTTISPRYVPADM
                     LGSADPAELERINPPTVLGRVPYGLSLTVDYTAPQGVKSVSCASHPAQVSIDGNRVRV
                     ELMGTDVQLDRDFVVNIALAQPFAPHAVVAQDGPEECAVMINLFPDPGRFARQPGEYI
                     FLLDRSGSMAGGSIAQALDALCLALRSMEAGDAFNIIGFGSRHEMMFPHSQPYTQASL
                     DQATRALESWDANMGGTELLAPLQAALAAKAGELPRQFMLLTDGQVSNEAACIAAVAA
                     HAGSVRMFTFGIGHGASEFLVRGLARACGGKAEFIHPNERIEPVVMRQFARAAAPFLR
                     NVRVDWGGLAPNLVAPAHIGSLFAGDRLTAYARVPAGDPAEPVEVAVLADGPQGTMRF
                     PVRVDLRERVDDAMSGDAGSGSSGTFHRGSGFSRTIVRMNAGPRAVPALMARAAIREL
                     EEGRGAEREAGSNQRARRGKGVDTKVLELALRYQLLSSQTSFVAVEERSAGEQAERAQ
                     LRRVPVALTHGWGGSDRLAMSASHSLQPMADMGIRAMFSRSASPRPAASDAPLLPGMS
                     ASVMEYAAIDMHCVPELIASFDAPASSRTQRTTASKTRAAPDAFMLLTLSQRADGSFR
                     LEPEALRAFALDANALARAAALLGGGDRAEAMVHTAVALALLERRFGDRQDEWRMLAD
                     KAARWMAGQAVALPAGETSWAGWAGVACAVG"
     gene            complement(9735..10058)
                     /locus_tag="PLW13_02750"
     CDS             complement(9735..10058)
                     /locus_tag="PLW13_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY69315.1"
                     /translation="MSLTRSFKHTVAARIERDPHFARSLLDEAATLFLSGEPDAARLI
                     LRDLVNATIGFEQLAELTHKPSKSLHRMLSPKGNPSMDNLAAIFDAVRNRLNVTIEVR
                     IVEAA"
     gene            complement(10055..10408)
                     /locus_tag="PLW13_02755"
     CDS             complement(10055..10408)
                     /locus_tag="PLW13_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQY69316.1"
                     /translation="MLIAAEEYVRPDGSCPYRSWFDSLDSQAAAKITVAKVRLELGNT
                     SKIKWFRGIGEYVIDWGPGYRMYLAKDGDELIILFGGGTKRSQQRDIDQALALHDEYK
                     ARKRLLAGTRKGRKP"
     gene            10594..10905
                     /locus_tag="PLW13_02760"
     CDS             10594..10905
                     /locus_tag="PLW13_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY69317.1"
                     /translation="MPAQYAPVAFDPDEYVAKRARVDSAFRKACRELEDEFAALDVLL
                     EARRAAGLTQAEVARRIGVKASALARIESSLVSRKHSPSLQTLRRYAAACGRRLSISI
                     N"
     gene            10955..11185
                     /locus_tag="PLW13_02765"
     CDS             10955..11185
                     /locus_tag="PLW13_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69318.1"
                     /translation="MQESKRAIVYLEPEIHDALRIKAAALRCPVSEIVNDVLRQLLGD
                     DVDDLAAFEERAEESEISFDALLRDLKKHGKL"
     gene            11293..11556
                     /locus_tag="PLW13_02770"
     CDS             11293..11556
                     /locus_tag="PLW13_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQY69319.1"
                     /translation="MLTRVFKSGNSLAIRIPKEMAIADVAEEVEIERIGNSLVVRPAH
                     RRSLSGIAEIFSLFSPDFMSGGREFHEELEREWDEPTQRSGKG"
     gene            11560..12060
                     /locus_tag="PLW13_02775"
     CDS             11560..12060
                     /locus_tag="PLW13_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQY69320.1"
                     /translation="MLYMLDTNICIYLVQRHPPEVFERLEAHDEGDVVMSVVTYAELR
                     AGLSIHAAGRSRDEQALLLLARRIPVLNFDAAAAERYGILRAAVRDRRRNALDRLIAA
                     HAISVGATLVTNNETDFVDYPGIEVENWVAPPGTISEPPPRSTRGPASSARKSVPRRV
                     ASRPKR"
     regulatory      12113..12190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     regulatory      12230..12337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12388..12633
                     /locus_tag="PLW13_02780"
     CDS             12388..12633
                     /locus_tag="PLW13_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69321.1"
                     /translation="MATHDDSIRNTQSFSTGELTVLGLFYAGVRARLLRARYQLDPWA
                     VVQRHRHLPREEFLRVSEQAERAAAHARVTEAAGRFV"
     gene            12840..14180
                     /locus_tag="PLW13_02785"
     CDS             12840..14180
                     /locus_tag="PLW13_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQY69322.1"
                     /translation="MQVALLAALVTEDPLLLIGRAGTGKTYLLNSISEALGLEHRHYN
                     ASLIAFDDLVGFPYPDDARESIRFLQTPATVWGAESVLVDEISRCKPEHQNRLFSLVH
                     ERRVQGIALEKLRYRWAAMNPAGAEQDGGEYLGSEPLDPALADRFAIVVEVGDWSELS
                     EAEQRLVASPAGEGSLARDGGRLRADIARWRGEFVARLPQCPLQIVEYARIVTTELGG
                     AGLRLSPRRARLIARSLLAASVIGGGLHEAAFRTVLECSLPQRASGECPPQAKVAAAH
                     RLAWNAALATGKTRWLQDFHLARRFAAKVRLLAESCPDADSGTLAVEQLLANEPPERA
                     AAFALAVYPAALKGALPVGAEGLGDLARRAAPLLHVDGKLEWREPLSQSGTSHPELPR
                     VAAAIDALECPARQARARQLLFWALLNKVAVRDPEALEHEFHEAVEYLAGRAAA"
     gene            14177..14647
                     /locus_tag="PLW13_02790"
     CDS             14177..14647
                     /locus_tag="PLW13_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69323.1"
                     /translation="MIPFPSCRRPPPLPPPLLRCNTGGPMSRTRAQALHASVFDGSGG
                     TLHFSRYLSVGLDFEGSDPLAAIEELFARAGIDDAEQLRARCEPPAEACLCRENRELP
                     VPRFAAATDAELLRQVLLLGLCTPHFYDPRRPVLLTRASLGRRLEIYVERGFYA"
     gene            14644..15999
                     /locus_tag="PLW13_02795"
     CDS             14644..15999
                     /locus_tag="PLW13_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69324.1"
                     /translation="MKITPVRIHALIAELIDDNPFACRALLQIVSTSFTTDVPTAAVT
                     CGGEPRLLLNPEFLERHCQTEAHVKAVICHEFLHVLLRHTEGRGPRSRAEHLALDAVI
                     NAIIHRALGKEYSSFMTAFYRRETGVARLLRAPRAEETGYYSEGYRHGSAAERKLLSA
                     WGGLYGGFLLVDDIHALALDLERHGDGAPRRGWIGDHCARDGGGDATPEPLSEPVRAA
                     LDRSLKSMNGSGIWRDPNGRGVGQEAYSARVTAAEAAVERWRREAWRVLVRHLVPDRR
                     APRLEEAPSEFRLPVLSPSDRRSFSRALWSPLLPEALWNSPRRTAGASAQVYLDVSGS
                     MSKEMPLIIALLARLGSHIRRPFWAFSDVVAPAVIRDGQLHADTTGGTSMACVLRHLA
                     QTRPRAAVVVTDGYIESLTQAQVCAAAGVRLHAIVTREGSPAALQRAGIGYTQLGRLP
                     S"
     gene            complement(16035..16535)
                     /locus_tag="PLW13_02800"
     CDS             complement(16035..16535)
                     /locus_tag="PLW13_02800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY69325.1"
                     /translation="MKARAGLSIELLGATHDRRSFSCGVSALDDYLRRYARQHAAANI
                     SRSYVAADGKRVAGFYTLAMAAVRRDNLPAREADRFPRFPLPVARLARLAVDVRCQRQ
                     GIGELLLADALQRCLRLSEQIGMIGVIVDAKDEIARGWYERFEFERLPDAPLTLWLPT
                     GAIARL"
     gene            complement(16532..16810)
                     /locus_tag="PLW13_02805"
     CDS             complement(16532..16810)
                     /locus_tag="PLW13_02805"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HQY69326.1"
                     /translation="MSEASERIDVRTSPAIKELIVRAASTAGVSVSTFLLAAAQERAQ
                     HLLAEQESFTLTAADWKAFVAALDSLEKPRPRLSAAMARHRERETAGS"
     gene            17037..18119
                     /locus_tag="PLW13_02810"
     CDS             17037..18119
                     /locus_tag="PLW13_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HQY69327.1"
                     /translation="MIRVSEMVLPGHPDKFCDQAADAIVAACYAADPAGYCQVEMSCW
                     SDQVYLTGGIATARPLAEPLDEIVRRTGRELGYVAGNAIEADRYVIHSSVCDLRTDAT
                     QWTRHVNDQCISVGWAGYDEKVAFLPPEHYLAHVFREALTQSFATGRLARQGPDGKLI
                     VRLRENGADWVLEHVLVTVQQLDASCITGVAASLCAELRDAYAALQARDARWVAGWRD
                     VDVLVNPNGPLVNGGSDGDNGQTGRKLAADYYGPRVPIGGGALSGKDLSHIDRAAAYA
                     LRQAAIHAVRGGAKECQLTGVWAPNVGVPLDVIWQLDGRGPRLANEWFAHGAIMERTD
                     VRAVDRHLGRGRHFFDLALPWNGAVP"
     gene            complement(18230..19174)
                     /locus_tag="PLW13_02815"
     CDS             complement(18230..19174)
                     /locus_tag="PLW13_02815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY69328.1"
                     /translation="MNHLHLGCTPWQFPLAGGADALARQAVLAEELGYESFWLPEHHF
                     GADAIPEPLMLLAAVAASTRRIRLATTSYLLPLRQPLQAAAQVAVLDQLSGGRVILGV
                     GRGFVPELFEAFQVKRSEKRELFAECLAGMRAAWAGEPVGSSGARLMPAPVQQPHPPV
                     WVAALGPKALAQAGSLGLPYLASPMEPLATLEDNYAQHRAACTGAGHPLPDAVPVMRT
                     IFVSDDTARIARVRELLERRMTAMQQQSSALVRSLPRGLDDWAIVGSPPQVRDTIARY
                     RERLGMTHLVATRLLLADIEGAECEDSLRALTELASAF"
     gene            complement(19176..19736)
                     /locus_tag="PLW13_02820"
     CDS             complement(19176..19736)
                     /locus_tag="PLW13_02820"
                     /inference="COORDINATES: protein motif:HMM:NF017819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HQY69329.1"
                     /translation="MKTITDIASASQVLAFWFEELTPAQWWRKDEALDARIAQRFGAT
                     LAAARAAELAGWRDSAGGRLAEVIVLDQFSRNVFRDRAESFAADGMALALAQEAVRGG
                     FDAQLAPNERAFVYMPYMHSESGQIHQDALRLFDQPGLENSLDFERRHHAIIARFGRY
                     PHRNAILGRESTAEELAFLQQPGSSF"
     gene            19902..20552
                     /locus_tag="PLW13_02825"
     CDS             19902..20552
                     /locus_tag="PLW13_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69330.1"
                     /translation="MKGKRKAVIDVVRDAIARGASTVEEVVKSVSDMPLKKLGKLDPT
                     KRLRKVQNESIGTVGEAIRDINEQVRAFAADKLAQARKAGLVPEAKPAGKKRAAKAAP
                     AKRAARAAPKKRAVKAAPAKAASTKAAPAKQRVAKAAPAKQRVAKASVTASAPAKRKA
                     SVAAPAASSVSKRPAVARKRVARPAPGVTTSAPGVATPPVDVIAPSQPGEPMTQSE"
     gene            20709..21242
                     /locus_tag="PLW13_02830"
     CDS             20709..21242
                     /locus_tag="PLW13_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69331.1"
                     /translation="MAKKKSLIHLIQDAIDKGATSAEEIHKAIADKPLKLLLKLDALS
                     GPAKKIKRIQDESIGAIYDLIREINEQVAKYATEMLAQAREASKGKTATSVRKRVAAA
                     VKRVEKAGASAKRQAKAAAKRAPKAAAKKRAAAVGAVKRTVKAATAKQGPAKKAPARR
                     GPKPGARARAKASATAP"
     gene            21325..22299
                     /locus_tag="PLW13_02835"
     CDS             21325..22299
                     /locus_tag="PLW13_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY69332.1"
                     /translation="MGGDVTKRPVAVVLGITADMAFAAANVLLGLERFPPADGYDVVI
                     FHNGVPAGDLALLARLRDCHFVAYRCAESIRRNIPAHALRAFSEIVYAKYECFELLNR
                     YSRVIWLDTDILIRGDTAELLERGASGAAFAREDKPLRFNFSRDIEGFDMDRPFFNAG
                     VFALSDALPGRESARDWLLDATVRHADRVIMGDQGILNLWLQHQRVEPAELLPEYNVF
                     RHRPGAARARVVHAVGHHKPWMDFADPQWNADYVRWLALGGSPCPVWKTVRFMAARHP
                     HPLRHPREIPSRTVQILCFLLRRRRQARMARVASRNASAGGVLDAASH"
     gene            complement(22264..23400)
                     /locus_tag="PLW13_02840"
     CDS             complement(22264..23400)
                     /locus_tag="PLW13_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69333.1"
                     /translation="MPDGSFADSTLGPRAGNTDCVVLDAATPPVDPRAFAAAIRLVLA
                     GQSGELARDRNGICYRVDLVRCDGSSRSAILKIPRPGPQRTNPETTFAWEARILASLP
                     AAGIPGAPALLGRVAHGDTHFLFLEDVPGNHPDPRVHPLDTRHVGALLEHFHAMDTRG
                     LMHYDLKPANILIDGAQVGFIDYEFARFRSHLNAYAPDSDAFCADFNVSINGFFPARS
                     NVANFEFRALDCYLRDAAAEPATADALLNDWLRGKSGYHRRMAAFLAQLRDSSAELMA
                     ITSGIPAAAARARLGAAAQFEVLLANLFAQPGAAVSRLERQLMAYRCAVFERHAPEAA
                     RLRATIQAGIGAARARGATLPDAYCRAIARVLELVGRSVEDAPG"
     gene            complement(23393..24112)
                     /locus_tag="PLW13_02845"
     CDS             complement(23393..24112)
                     /locus_tag="PLW13_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQY69334.1"
                     /translation="MRYLILAAGVGKRMGSALAGLPKCMIDIDGEPLIARLLRQIRQF
                     DAAPEIHVLLGYRSETIAPLLEGCRIIINPFFDITGINASLWFARESFNRALLFMHGD
                     LVLSDKLAQALFTAPEASLVTYDSNVMDPREINIRAEGGRVTRFGVNFTGYSGAYAGA
                     IKLSAQAARLFADTLDQRIRRGFNEPRTYYFFVMRRLIAHPDVMLAPFDFAPFRWQEI
                     DYARDIAAARLCVSAGGSRDA"
     gene            complement(24146..25474)
                     /locus_tag="PLW13_02850"
     CDS             complement(24146..25474)
                     /locus_tag="PLW13_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQY69335.1"
                     /translation="MKNLLILHLESITRHNLAIFGSAFANIRRLMQDAVVFENFFSSA
                     TSTLMAITYLLHGNDLEFDTSAEFEGIQPARNTRNLFSILQERGYDTRFICLNGFHAN
                     DETRLASWPADLPPVWATNDFPTLFRRFDELTDAAPFAIYAWELVSHIEHSLALAPFS
                     ASLTDQVRRACGVADHAVGEMMGILARKGLLDNTTVVMFGDHGDDFWTHGFKGGMIHG
                     TEPYTQITWTPCAIRDPSLTPSSRANLASTIDIAPTCLALLGIGEPGGFGYGGLNLLT
                     QQQDIVFSQNFTANQPDNRKTGVTKAFAATDDTYVLLASSRGLEMYAWRLDPGNHCNL
                     LQFFDLGHDGELALRERPDAAAHFKAAMTNNPRAVASIAERFRALRAALSARIAAKRA
                     HIEASGVEAQFALDPQCLGIVAGESDGTAPIRPAATTAPQTTFSYSIQLP"
     gene            complement(25670..26566)
                     /locus_tag="PLW13_02855"
     CDS             complement(25670..26566)
                     /locus_tag="PLW13_02855"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQY69336.1"
                     /translation="MKYFKRALLAAAIAAPAALHAAPQVTIDAPAANSIQSGITQIYG
                     WASDVEPVVKVELYIDGQESHTMRIGMGGERADVAAAFPGDAEPLHSGFATAFNTKLL
                     SNGPHTITIEATNQLGEKTELTRTIIVSNAPGAESQSEVVDLSGATARISGDHIFLDG
                     VLINGTVYNNFELRFDDVINGFRVIGFRDDEDGDGFSDDDSDKDGFRDDDSDKDGFSD
                     DDDDKDGLDDDLDDDDSVDDCDDDVDPLCDDDSDDDSDDDSNDDSHDHSDDDSDDDSD
                     DDSDDDSDDDSDDDSDDDSDDD"
     gene            complement(26819..28921)
                     /locus_tag="PLW13_02860"
     CDS             complement(26819..28921)
                     /locus_tag="PLW13_02860"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HQY69337.1"
                     /translation="MSLLSRLPLAIAMLALGAHGASASTAYGDLNNFDAVNDTGQECH
                     GFEIEIHDIRSTDITYTYDWNHYGAPKIREDLSDPAHPKVFVRYESTKNPDGTWAAYT
                     ATPAGPLAPTDGHSCTNPSVNDGCEHFGVGYYGTPTALRYNWLIDDGAGNLVLGPPVA
                     VATPTWTYNPPVVVDGNIEQPAEVVAAIPAPEIEVPPNKEFGEPVWMKVIKTTTHNPD
                     PVALEDLISDDLDGDGQAEWQNREPDEVESEWTLVQNRADGGDVVEALAPDEMGDGSE
                     VVTRRYEFYRYVDDNPIGVGSIDGESGEAMCDKVAADGIHGVGTRNVTNAGGGSYEFD
                     CSQLAVVGDYIGAQMAAFAAEAPLGLIDHLQDAEPAAPYTPRTVVIGGNSPYAISLGN
                     GALPPGMVLGAYQDPETGAEIPGVLSGTPTAEGRYAFTVAVTDADGMSVSQAYTLQVG
                     AGAPAPLQYSLGVGRSGTGSGSVSGSGIDCGATCSITLEAGSSATLNATPAADSLFAG
                     WSGAGCAGTGACSVTLTNADAYVTALFDKKQFTLSAAKSGSGTGRVSGNGIDCGSACT
                     ATLDIGTAVNLTATPDAGMVFSGWSGACTGTAACSFSLAANATATATFAPAPAPSKYT
                     LSVVKSGSGSVSSKPSGISCGTLCSASFSTGSTVTLTAKPSSRKVVFAGWSGACSGTS
                     LSCKVTMSKTQSVTATFK"
     gene            29140..29487
                     /locus_tag="PLW13_02865"
     CDS             29140..29487
                     /locus_tag="PLW13_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQY69338.1"
                     /translation="MRPLVVCAVVTFLGACSGTSGPIIDTKGVDLAQFERDKAECEAF
                     ATQVSTGQAVGRGAAGGAAVGAAIGAITGSAGRGAGIGAVTGGAESARISERERVNVV
                     SNCLRGRGYRVLN"
     gene            complement(29575..>29864)
                     /locus_tag="PLW13_02870"
     CDS             complement(29575..>29864)
                     /locus_tag="PLW13_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HQY69339.1"
                     /translation="PPGLGGLPPGLGGLPGQPGGPFIPPIELPIELPIELLIPPTLPG
                     KSFPPQGQPLETFDAPEQLANGTVPEPGTLPLLAIGIGAALYIARRGKNRK"
BASE COUNT         4859 a         9344 c        10430 g         5231 t
ORIGIN      
        1 ggcgcgcgac agctcgcgat catcgactgc gactaccact acggcgacgg cacccacgac
       61 atcctgttgc gcctgggcct gtacgaccac gtgcggcact ggagcctggc cgctcgcagc
      121 ggcagcgagc gccaggcgct ggcgcggctg gaagccgcac tcgccgagaa cctgccaggc
      181 agcgaattgc tgatctacca ggccggcgcg gactcgcatg aggacgatcc gctgggtgga
      241 gtgttcacga ccgcgggata ccgtgagcgc gaccggatgg tgttcacggc ggcggcgaga
      301 gccggggtgc cggtggcgtg gaacctggcg ggcgggtacc aggagccgct ggggaaggtg
      361 ctggagttgc atgcgattac gatgggggag gcgttggtgg cggaaggggt cggtgccaat
      421 accgcattgc atgcggggca ggaacagtga aagagaagaa atgaaattac ttcgtgatcg
      481 attgagcgca acccatatgg gtcgtacatc tacgcttgta attatgagcg cgcggcgcaa
      541 tccccatggg gaaaatctct tgcgccgaag gcggatttgc cttgcgagtt agtgctttga
      601 atgctattcc ctcaaccatg aaagctactt ggattaaatg ggctgctagg cacctgatgg
      661 ctcgatattg cctaacgttt gaggatattg gtcagtcacc atgcgaattt gaaaggcgtt
      721 ggcgtgatca ggcggcgacg gcagaagacg ctgtcgacgc ttttgcagaa aaatatgatc
      781 tcgatccagt cgaagcacga cattaacgcc gcgaatggaa ttggcgcagg atatgaattg
      841 aagcagctaa tcgaattcgc atctggcaag ccaagctgca atcgattgaa cgaaaaatct
      901 gatatgaacc gctagaaatt gtgcataccg attccgtact caatcttccc gatgaatcca
      961 gaatgctcga tgtcgttcgg cgtggcctgg aatccgctga cgacgttcga attgtggtgt
     1021 cctttacgag atgtagcggc ttggggctat tgatcgattc gcttcgacag gtggctgagc
     1081 gcggtgggaa aatacgcata cttacctcga cgtatcagtc cgttacgcag cccgaagcgc
     1141 tggatgtgtt gcttcaactt ccgcgcgtcg agactcgagt gcagatgggt tccgtagggc
     1201 tacatgcgaa gttttggtgg ttctatgggg accgtggggc ggaatgttgg gcgggctcct
     1261 ctaatctatc caagggcggt ctcgcgacca atatcgaatg gaatgtccgc cgaattgact
     1321 cggccgtaat tcaggaaaca cgtcatcagt tcgatcgtct atggggcaga gctgacgtgc
     1381 gagagctgga cgatcaatta atccgcttgt atcgaaaatc ctacttggaa aacgcagcag
     1441 ccaatatggt gcgtccggcg cgggtcgaat accaccttga tccggttaat ccgaacgaag
     1501 cgcaagtcga agcattaacg cgtctcgcgg agttgcgagg gaggggggag cgtcgcgcag
     1561 cggttatcgc tgcgacgggt gttggtaaga cctacctggc tgcacttgat gttcttcaga
     1621 gcgggtcgcg tacggcgctt tatatatcgc atcggcttga gcatctgcat caggcgcgcc
     1681 gggcattttc tcgggttttt ggagacagcc ttaagctcgg tgttgtaggt ggagggcttg
     1741 atgaggcgga tgctgacatt gtttttgcga cgatttcgag catcgcaacg cgtcccaacc
     1801 tcgctgggag aaaatttgac tatctcgtgg tcgatgaatt tcatcatgtg gaggcgccaa
     1861 gctatgacgt gcttcgagct ttgcgagatc aagcatttct tcttgggata accgcgaccc
     1921 cggagcggca ggatgggcat gatgtcctgg cgtggtgtga ttggaatgtt gcatatgagg
     1981 ttcgacttcc ggaagcgatt gataggggct ggttgcttcc gtttcactat ttcggcatcg
     2041 ctgacgagac ggtcgacttt ctaaaaatcc cttggcgcag gctgaatgaa gttgaggatg
     2101 ctctttctgt agacgcacgc gcagaacacg ttttgcatca tgcgcttgag cgcggtttcg
     2161 acggaccaaa gcgtgcgacg gtcggcttct gcgccggacg taagcatgcg aaattcatgg
     2221 ctgcagcatt tgtccgtcgc gggcatgccg cggtcgctgt tctaggtgac caaactgttg
     2281 aacggcgaga agaggtttac cgtcagcttg ccgatattga tgacccgctt caatgggttt
     2341 tcgtttcgga cgttttgaac gaaggcgtgg atattccggc cgtcaattcg gttctgttct
     2401 tgcggcccac agagtcagca acgttgttct tgcaacaact cggtcgcggt ctcaggcttt
     2461 ctccgggcac tgaggtgcta acagttctcg atttcgtcgg ccaccatcgc tcggcgtggc
     2521 taacgttgaa tgcgctaaat gctccatccg gaggcgggcg gcgaaccgag attgcagacg
     2581 gtgtcacgat caagccgccc gccgcttgcg aagtagtgct tcagccgcgg acgcgtgaga
     2641 ttctctcaaa ggtggctaga tttacaacaa ggcgcgaagc ttgcgatgaa gcctacatca
     2701 gattgcgcgc cgagttgggg cgtgttccta tgcctgtgga tctctggaat cgggctgatg
     2761 tgccggagtt gcgagatttc cgtgctgctt accgagggtg gcttgattgc cagatcgcca
     2821 acggtgacac ccctgaatgg gtgatcggcc tagcgcaaga tcactccgtt cgcgattttc
     2881 tccgaattgt tgaatcggat tggcaggctc agcgggtatc gccgtatgcg ttgttgtggg
     2941 ggctgtgtgc ttcgcctgat tttcccgaag tgggatacga acaatttttc gaccgttgga
     3001 tgcaatggaa atccgagcgt tcggcgcttg attcgtccag aacatggagc accctgcgca
     3061 ggaagcttgg gtcagcgctc gaaggcgacc gaattgcagg ttctatccga gaggcgctca
     3121 gtgagcatct gcttgctgag gttgaaggga ggcttctata tacggtgaac aatgaccacc
     3181 ggcagcgaca tgggggcgtg ctgcgctcgc cctcagatct gaatttgttc gcaatgtatg
     3241 atcggccaga gatcgttcgc cacttcggcg ttcactacga tccagcgaag cacaatacgg
     3301 ggatgctgtg gttcggtggc gatggcgtaa ttatcacgaa gctcgatacg tcgacagcga
     3361 ttgcgcgcca tcagtatcgg aatcgtctca ttgacgatcg gacattctcg tggacaagcc
     3421 aaaatcgaat gggcacggga aacgaggcgg ggctaaaggt cgtagagcat gaaatgcgtg
     3481 gattgcgtct ctaccttttc gttcaaccgc gctctcacgc gcctgcttgc tatcttgggc
     3541 tagtgagggt ggcaaaacga gctgatgcgg aggggtcagg ccccatgcag gttagatttt
     3601 cgctcagtcg atcggtgccg gctgatgtac ttaaagagct tctggagtct gattcagagg
     3661 cgctcgtggt gggcggggcg gatgactagt ttgttgtcgt tacgcaagtt ggttgttggg
     3721 gcttgatggt tgcggatgga acgtcgcact ggcacctaca catgaatcgc tgcccatttt
     3781 gcgctttgag tggagacagg attgttgcgt ccgacggatt aagtgtagtt ttccgcgacg
     3841 gcttcccaat ctccctggga cacactctga taatcccccg ccgccatatc ggctcgttct
     3901 tcgaggccac ccccgacgag cgcgcatcac tgctacatct gctcgaccaa gcccagcagg
     3961 cgctggcttc tgagttccac cccgacggct ggaacatcgg catcaacgac ggccccgccg
     4021 caggccagac agtgccccat ctgcacatcc acctgatccc gcgctacacg ggtgacagcg
     4081 ccgacccgcg cggcggcgtg cgttgggtta tcccgggcaa ggcgaattac tgggacagtg
     4141 gctcaggcaa tggaaatccg tgattcaggg ggcggggcag cctattcggt tgcaggggta
     4201 atccggccgc cgccgccgga ggagcggttg gcgtttttgc gcacgctgca gcgcgtcctg
     4261 aaggaaggcg acttcaccgc gacgtacaag ttcgctctgc tccaggcgct gctcgatatc
     4321 gcggtggaat cgcggatccg gaacgacgcc gagttgccgg tgagctaccg ccaacttgcc
     4381 gagcgtttca tcgagtatta ctggggacac accgagccct accggcctgg agacgagagc
     4441 ggcggcgtcc tcgcgcagtc taccgggcgt caggcagccg tgctcaccct gctagccgcg
     4501 gcgcgcgggc aaagcggctc gcgcacgctg gcgcaactgc gcagggagcc gcgttacccg
     4561 ggcttgcttt ctcgggtcgc cagagtcatt cgcgagcagc cggtcaggta catccagaac
     4621 ctgaatggtg ggcatatcga gtttctgttc gctcccgcag ccgacggcaa gggcatcgtc
     4681 ctcaagcgcg gcgttgcgtg gtgcctgcag gagttctatg acctgctgca gagccaggtg
     4741 cgtgcgggtt gggtgcagca ggtgcggtcg atgtcgcgca atgcctcgtt gatcggtggg
     4801 cacaaggatc tggaggaatt cctgttcggc cagcagcgca tggcgctcgt caggattcgc
     4861 gaacggctgg tcgacctgga agggccgacc tgcttctact gtgagaagtt gattgcccat
     4921 gggattgagg tcgaccactt cgtgccctgg tcgcgctacc cgcacgatcg cgggcacaac
     4981 ttcgttctgg cccatgcggc ctgcaaccac agcaagcgcg acacccttgc cggcagggac
     5041 cacttggagc gatggctggc gcgcatcgac gacaaggctg ggccaatttc cgatgcgctc
     5101 tcagacgtac tcctctgcga tgcggcgagt tccgcaaagg tggcgcagtg ggcctacctg
     5161 cacgagatcc acgcggcggg catgttctgg gccggtttcg cggggcgctc gccggtgtat
     5221 cagccggtgg atgatgcgct gcgcagtctg gtcgtgagcg gctttcccat gtgagcgtgg
     5281 ctcgccgctc tgtctcattc ccgttttggg cgggcttgaa atgtgccagc gtctctagaa
     5341 gttcgagtgg gtgctgccac gactatcagg gcgtgctacg cctatgccgc caccgcgtcg
     5401 caagcatccg cgcagtccag gcacgcgtcc tcgcaggcct tgcactcctc gtggtgcgcg
     5461 gagtgtggct tgcaggcctc ggcgcagctg cggcagaact gcgcgcaggt cttcgccagg
     5521 gccttcaggt cggccggctt ggcattggcg tggccggcca ccgcgaaggt cgcctgcacg
     5581 accggcagca tgttcatcac cgagcgctgg cagtcggcca tcaacggggc gccggtggcg
     5641 aggtggtcgg tgcagcgcgc catgcagaaa ttgcccgcct tgaggcacgc ggcggtggcg
     5701 tcgatcaggc gcttgtgcgc agccgacagc acgggggccg gcgccgcgtc cttcatgtca
     5761 tggcccgcat gggcgaccga cgcgtcgggc tgggcgaatg ctgccgcgcc ggcgagcgcg
     5821 gcgccgaggc ccgccagccc ggcgatgacg tcgcggcgct ggtgcagggt ttcgttcgac
     5881 atggagacct cctgttccgg tgtacgtcgg gttgcttgtg cgtgcgagtc ggatggagtc
     5941 gcgacgcggc gttgctcggc ggggattctg tgcccgtcat ggtggtgatg caaccggtcg
     6001 gccatcatcg gccggtgccc gtgtcgcgct gcgcgatctc gtgcaccgtg ccgctgccga
     6061 tacccggata gatcccgctg cgctgcacgt gtgcgggcga gggcgcgacg gtgacgatca
     6121 tcaccgaggc cacctgcccc gcgccgttca cggtgagcac ggccgttagc ctggggcgcg
     6181 ccagccagtt gcgctccgcc gcctccaggc gctcgaagcc ggccaggcac tcgccgaagg
     6241 tcggggagcg cgcgagctcg aagcgcggct cgcggcccgg cagtgcgcgc agggcctcga
     6301 tcttgcggcc caggtgctcg cagaccggcg tgcgcccgtg cacgatgtcg aacacctgct
     6361 gcgagccgta ctgcacgtcg tcggccagca gccccgccag ccagttgcgc tccagcgtgt
     6421 tgaaggcgag cgcgtagcgc acgcaggcct cggcaagcgc cggcttgtgg ctcgtggcga
     6481 tccattcggt cgtgcgggga tcgtggggta gtggacgcat gcgggtctcc gtactgcctg
     6541 gattttttca gtatggtccc gcccggcctc agggcatgag gctgcgcgga gatgcattga
     6601 tgcagggcaa ccgggtactg aattcggtgg tcttgcgggt tcgcaaatcc cctcttgcca
     6661 tcactctccg gtaacgttat aaatgtgaca gttgaatgtg acagtaactg ttatgaaaga
     6721 gccagtgacc gaacctcgct attcaatcgg cgaactcgcc gagttgggcc gcgtcagccg
     6781 tcgcaccgtg cacttctacg tgcagcaggg gctgatcgac ccgcccgagg ggcgcggccg
     6841 cggcagttac tacaccgacc ggcatctcga gcagctgcag cgcgtgctgg cgctgcagcg
     6901 cgaggggctg ccgctgcgcc gcatccaggg catgtccgaa tccgggcagc tgcgtgccgc
     6961 cgaggcggtc ctcgcgcggc aactggtgtt gcggctcgcg gtggcgccgg gcgtcacgct
     7021 ggaaatcgag gcagggtccg gcggcgagat cccgtcggcc gagcagctca gggaattggc
     7081 gcggcgctgt gccgaggtgc tcgggaacga ttcgtagtca cgcttaccgc aacgggtccg
     7141 ggcggatccg gcggccgtgc aaacaacagg agcaagcagt catgggaacg atggaacagg
     7201 cagcgggcgg gatgttcgtc aggagctcgc aggcgccggt gccgctcacg ggcgtgagca
     7261 tcgaggtcac gggcagcggc agcgccgccg aggtgtgcgt gcggcagcgc ttcgtcaacg
     7321 tggagcgcgt gcccatcgag gcggtctaca gctttccgct cgaggaaggc agcgcggtct
     7381 gcgaactggt catggagacc ggcggcaagc ggctgctcgg gcgcatcgag gaacgggaaa
     7441 aggccttcga acagtacgac gaggcgctgg ccgccgggca cggcgccttc ctggcggacc
     7501 aggaccgccc gaacatcttc accatcagcg ccggcaacct gctgcccggg caggaggcca
     7561 cggtctgcct gcgctatgcc accgagctgg agcagcatgg aggcgagctg cgcatcatgc
     7621 tgccgaccac gatttccccg cgctacgtgc cggccgacat gctcggctcc gccgatcccg
     7681 ctgagctgga gcggataaac ccgcccaccg tgctcgggcg ggtgccgtat ggcctgtcat
     7741 tgaccgtcga ttacaccgcg ccgcagggcg tgaaatccgt gagctgtgct tcgcatcccg
     7801 cacaggtgtc gatcgacggc aatcgcgtgc gcgtcgaact gatgggcacc gacgtgcagc
     7861 tcgaccggga cttcgtggtg aacatcgcgc tggcgcagcc gttcgcgccc catgccgtgg
     7921 tagcgcagga cggcccggag gagtgcgcgg tgatgatcaa cctgtttccc gatcccggcc
     7981 gctttgcccg ccagccgggc gaatacatct tcctgctgga ccgctccggc tcgatggccg
     8041 gcggcagcat cgcgcaggcg ctggatgcgc tgtgcctggc gctgcgttcg atggaggcag
     8101 gcgacgcctt caacatcatc gggttcggca gccgccacga aatgatgttt ccgcacagcc
     8161 agccctatac gcaggcatcg ctggaccagg cgacccgggc cctggagagc tgggatgcga
     8221 acatgggcgg caccgagctg ctggcgccgc tgcaggccgc gctggcggcg aaggcggggg
     8281 agcttccgcg ccagttcatg ctgctgaccg acggacaggt cagcaacgag gccgcgtgca
     8341 tcgcggcggt ggccgcgcac gccggctcgg tgcggatgtt caccttcggc atcggccatg
     8401 gcgcctcgga gttcctggtg cgggggctgg cgcgtgcctg tggcggcaag gcggaattca
     8461 tccaccccaa cgagcgcatc gagcccgtcg tgatgcgcca gttcgcgcgc gccgcggcgc
     8521 cgttcctgcg caacgtgcgc gtcgattggg gcggacttgc gccgaacctg gtcgcgccgg
     8581 cgcacatcgg ctcgctgttc gcgggcgatc gcctcacggc ttatgcgcgg gtcccggctg
     8641 gcgaccccgc ggaacccgtc gaagtcgcgg tgctggcgga tgggccgcaa gggacgatgc
     8701 gttttccggt gcgcgtggac ttgcgcgagc gcgtggacga tgcaatgtcc ggtgacgcag
     8761 gttcgggctc cagcgggaca ttccatcgtg ggtccggctt cagccggaca attgtccgca
     8821 tgaatgcggg accacgggcg gtgcctgcgc tgatggcgcg cgcggcaatc cgcgagctgg
     8881 aggaaggccg cggcgcggag cgcgaagccg ggtcgaacca gcgtgcgcgc cggggcaagg
     8941 gcgttgacac gaaggtgctc gagctcgcgc tgcgctacca gctgttgagt tcgcagacga
     9001 gcttcgtggc ggtcgaggag cgctccgcgg gcgagcaggc ggagcgcgcg cagctgcgac
     9061 gcgtgccggt ggcgttgacg catgggtggg gtggatccga ccggcttgcg atgtccgcaa
     9121 gccacagcct gcagccgatg gccgacatgg gcatccgggc gatgttcagt cgcagcgcgt
     9181 ccccgcggcc cgcggcatcc gatgcgccgc ttttgcccgg catgagtgcc agtgtcatgg
     9241 agtacgccgc gatcgatatg cactgcgtgc cggaactgat tgcgtcgttc gacgcgcccg
     9301 cgtcttcgcg cacgcagcgg acgactgcat cgaagacccg cgcggcgccg gacgcgttca
     9361 tgctgctcac gctgtcgcag cgcgccgacg gcagcttcag gctcgagccg gaagccctgc
     9421 gcgcgttcgc gctggacgca aacgcactcg cgcgtgccgc cgcgttgctg ggtggcggcg
     9481 accgtgcgga ggccatggtg cacacggccg tcgcgctggc gctgctcgag cgccgcttcg
     9541 gcgaccgcca ggacgaatgg cggatgctcg cggacaaggc cgcgcggtgg atggcggggc
     9601 aggcggttgc gttgccggcg ggggagactt cgtgggcggg gtgggcgggg gtggcgtgtg
     9661 cggttggttg atgggtgtgc ggccgccacg atgtggccgc gcccgtaatt cacgccacgc
     9721 gtgtcatcgc acggctacgc cgcctcgacg attcgcacct cgatggtaac gttgagccgg
     9781 ttacgcacgg catcgaatat cgcggcaagg ttgtccatgc tcgggttgcc tttcggcgac
     9841 agcatgcggt ggagactttt gctcggcttg tgagtgagct ccgcgagttg ttcgaagccg
     9901 atcgtcgcgt tgacgaggtc gcgcagtatc agccgtgccg catccggctc accactcagg
     9961 aacagcgtcg ccgcttcatc cagcagcgat ctggcgaagt gcgggtcgcg ctcgatgcgc
    10021 gcggcaaccg tgtgcttgaa actccgggta aggctcatgg cttcctccct ttccgagtgc
    10081 cggcaagaag ccgttttctt gccttgtatt catcgtgtaa tgccagcgct tggtctatat
    10141 ccctctgctg gctgcgcttg gtgcctcccc caaagaggat gatcaattcg tcaccgtcct
    10201 tcgcgaggta catccggtac cccggacccc agtcgatcac atattctccg atccctcgga
    10261 accatttgat tttcgacgta ttgccgagtt cgagccggac ctttgcgacc gtgattttcg
    10321 cggccgcctg agaatccagg ctgtcgaacc agctgcggta ggggcagctg ccatccggtc
    10381 ggacgtattc ttcggctgcg attagcatac gccgaaagta acctaaacgt tactgtatgg
    10441 caagctgcga aggacgctga catccatcgg cgcagagcca gacttggttg ttgttaacat
    10501 ggatattatc ttcagccgtg agtttggtcg ttgcgttgga gatcgcccgg aagagattag
    10561 aggaggttcg cagtggctag acgggttcgc gagatgccgg cgcagtacgc tccggttgcc
    10621 ttcgatcccg atgaatatgt tgcgaagcgc gcgcgagtcg attcagcttt caggaaagca
    10681 tgccgggagc tcgaggacga attcgcggcg ctggatgtgc tgctggaggc gcgtcgggct
    10741 gccgggctca cccaggcaga ggtggcgcgg cgcattggcg tgaaagcttc ggcgctcgca
    10801 cgcattgagt cctctctggt gagtcgcaag cattcgccgt cgctgcagac cttgcgacgc
    10861 tacgccgcag cctgcggcag gcgtctgtcc atttccatca actgaaatcc cgttgggaat
    10921 gtaggcgtcc tgaaagagca accgaggacc ggttatgcaa gaatccaagc gagcgatcgt
    10981 ctatttagag ccggagattc acgatgcact gcgcatcaag gccgcagccc tgcgttgccc
    11041 ggtttcggag atcgtgaatg acgtcttgcg acagctgctg ggcgacgacg tggacgacct
    11101 ggcggcattc gaggagcgcg ccgaggagtc cgagatctcc ttcgacgccc tgctcaggga
    11161 cctgaaaaag catggaaagc tataggctcc taacgtcggt gcgcatcgca gggacggtta
    11221 ccgacactga gtggtgtcgc cgatcgagat cagtgtatat actgtatata catggctgct
    11281 gaggggcgtc caatgctcac gcgcgtattc aaaagcggca actccctggc catccggatt
    11341 cccaaggaaa tggcgatcgc ggatgtcgct gaagaggtcg agatcgagcg gatcggaaat
    11401 tcgttggtgg tgcggccggc tcatcggcgc tcgctttcgg ggattgccga gatcttttcg
    11461 ctgttcagcc ccgacttcat gtcgggaggc agggagttcc atgaagaact cgaacgtgaa
    11521 tgggatgagc cgacacagcg ctcgggcaag ggctgacgca tgctttatat gctcgacacc
    11581 aacatctgca tttacctcgt gcagcgtcat ccgccggaag tattcgagcg cctggaggcc
    11641 cacgacgagg gcgacgtggt catgtcggtg gtgacgtatg ccgaattgcg tgccgggctt
    11701 tcgattcacg ccgcaggcag aagccgcgat gagcaggcgc tcttgctgct tgcccggcgt
    11761 atcccggtgc tgaacttcga tgcggcagcc gctgaacgct acggcatttt gcgggcggcg
    11821 gtgcgcgatc ggcgccgtaa tgcgctcgac cgcctgatag ctgcccatgc aatcagcgtc
    11881 ggagccaccc tggtcacgaa caatgaaacg gactttgttg attaccctgg cattgaggta
    11941 gagaactggg ttgcaccgcc aggaacaatc tccgagcccc cgccgagatc gacgcgaggt
    12001 cctgcttcct ccgccaggaa atccgttccg cgacgagtcg cttcacgccc gaagcgttga
    12061 tgcactctgc aaccgccgcg gcgcttgcat ccggaccacg accacggcta cattacctcc
    12121 aagcgccgat ttgaacctca gcttgcgggc gctctaaaaa tgcagctaaa gcggtcttct
    12181 ccggaaaccc gctttggcac gcaagccgag cgggtttttt gttttctgga ctacgcatcg
    12241 cgagtgggct gaacagctcc gccaacctgc cgacctgggc aaggtggtat tccccgcagg
    12301 gggagatgtg gccgtaaggg caaccaagca ggttcacctg tgcctccggc tcctcgcgtc
    12361 gcgtgcaaac aagcgaagga gcacacgatg gctacgcatg acgattcgat ccgcaacacc
    12421 caatcgttct cgaccgggga actgacagtc ctggggctgt tctacgccgg tgtccgcgcg
    12481 cgcctgttgc gcgctcggta ccagctcgat ccgtgggccg tggtgcagcg gcaccgccat
    12541 ctgccgcgcg aggagttcct gcgcgtatcc gaacaggccg agcgagcggc ggcccatgcg
    12601 cgtgtgacgg aagcggctgg gaggttcgta tgagcgcgcc cgttacggcg aagttgcggc
    12661 gggggaaggg ggtaagcaaa agtggtgtga gcaaacaggg aggcccttgt acgcatgaat
    12721 gcggacccac ggagacaagt ccgcctgaag gtggagccac aggaaggcgg aagagtccga
    12781 ataaatccgg acccacgggc gaccgctttg cggggatcgg ggcctacggg ttcaaaaagg
    12841 tgcaggtggc gctgctggcc gcgctggtca ccgaggatcc actgctgttg atcgggcgcg
    12901 cgggcaccgg caagacctac ctgctgaaca gcatcagcga ggcgctggga ctggagcatc
    12961 gccactacaa cgcgagcctg atcgccttcg acgatctcgt gggctttcct taccccgacg
    13021 acgcgcgcga gagcatccgt ttcctgcaga cgccggcgac ggtgtggggc gctgagtcgg
    13081 tgctggtcga cgagatcagc cgctgcaagc ccgagcacca gaaccggctg ttctcgctgg
    13141 tgcacgagcg ccgcgttcag ggcatcgcgc tggagaagct gcgctaccgc tgggcggcga
    13201 tgaacccggc cggtgccgag caggacggcg gcgagtacct cggcagcgag ccgctggatc
    13261 cggcgctcgc ggaccgcttc gcgatcgtgg tcgaggtggg cgactggagc gagctcagcg
    13321 aggccgaaca acgcctggtc gcgagcccgg ccggcgaggg ctcgctggcc cgcgatggcg
    13381 ggcggctgcg tgccgacatc gcgcgctggc gcggcgagtt cgtggcgcgg ctgccgcagt
    13441 gtccgctgca gatcgtggaa tacgcgcgca tcgtcaccac cgagctcggc ggcgcgggcc
    13501 tgcggctgtc gccgcggcgc gcgcggctga tcgcgcgcag cctgcttgcc gcgagcgtga
    13561 tcgggggcgg gctgcacgag gcggcctttc gcaccgtgct cgagtgcagc ctgccgcagc
    13621 gcgccagcgg cgagtgtccg ccgcaggcaa aagtcgcggc ggcgcaccgg ctggcgtgga
    13681 atgccgcgct cgccaccggc aagacgcgct ggctgcagga cttccacctg gcgcggcgct
    13741 tcgcggccaa ggtgcgcctg ctggccgaga gctgccccga cgccgacagc ggcacgctgg
    13801 ccgtggagca gctgctggcg aacgagccgc cggagcgcgc cgcggccttc gcgctggccg
    13861 tgtacccggc cgcgctgaag ggggcgttgc cggtgggcgc cgaggggctg ggcgatctcg
    13921 cgcggcgcgc cgcgccgctg ctgcacgtcg atggcaagct cgagtggcgc gagccgctgt
    13981 cgcagagcgg cacctcgcac ccggagttgc cgcgcgtggc cgcggccatc gatgcgctcg
    14041 agtgcccggc acgccaggcc cgcgcgcgcc agctgctgtt ctgggcgctg ctgaacaagg
    14101 tggcggtgcg cgaccccgaa gcgctggagc acgagttcca cgaggccgtg gagtacctcg
    14161 cggggagggc ggcggcatga tccccttccc gtcctgccgg cgtccgccgc cgctgccgcc
    14221 gccgctgctg cgctgcaata ccggcggccc gatgagccgc acgcgcgcgc aggcgctgca
    14281 cgcctcggtc ttcgacggca gcggcggcac gctgcacttc tcgcgctacc tgagcgtggg
    14341 gctcgacttc gagggcagcg acccgctcgc ggcaatcgag gagctgttcg cgcgcgccgg
    14401 catcgacgac gccgagcagt tgcgcgcgcg ctgcgagccg ccggccgaag cctgcctctg
    14461 ccgcgagaac cgcgagctcc cggtgccgcg cttcgccgcc gccaccgacg ccgagctgct
    14521 gcgccaggtg ctgttgctgg gcctgtgcac gccgcacttc tacgacccgc gccgccccgt
    14581 gctgctcacg cgcgcgagcc tggggcggcg cctggagatc tacgtcgagc gggggttcta
    14641 cgcatgaaga tcacgcccgt caggattcac gcgctgatcg ccgagctgat cgacgacaac
    14701 cccttcgcct gccgcgcgct gctgcagatc gtatcgacca gtttcaccac cgatgtgccc
    14761 acggccgcgg tcacctgcgg cggcgagccg cgcctgctgc tgaacccgga gttcctcgag
    14821 cgccactgcc agaccgaggc ccacgtgaag gccgtgatct gccacgagtt cctgcacgtg
    14881 ctgctgcgcc acaccgaggg ccgcggccct cggtcccggg ccgagcacct ggcgctggat
    14941 gcggtgatca acgccatcat ccaccgcgcg ctgggcaagg aatacagcag tttcatgacc
    15001 gcgttctatc ggcgcgagac gggcgtggcg cgactgctgc gcgcgccgcg cgccgaagaa
    15061 acgggttact actccgaggg ctatcgccac ggcagcgccg cggagcgcaa attgctgagc
    15121 gcctggggcg ggctctacgg cggcttcctg ctcgtcgatg acatccacgc gctcgcgctc
    15181 gatctcgagc gccacggcga cggcgcgccc cgccgcggct ggatcggcga ccactgtgcg
    15241 cgagacggcg gcggcgacgc gacgccggag ccgctgtcgg agccggtgcg cgcggcgctc
    15301 gaccgctcgc tgaagagcat gaacggctcc ggcatctggc gcgatcccaa cgggcgcggc
    15361 gtcgggcagg aggcgtactc ggcgcgcgtg acggctgccg aagctgcggt ggagcgctgg
    15421 cgccgcgagg cgtggcgcgt gctggtccgt cacctggtgc ccgaccggcg cgcgccgcgc
    15481 ctggaggagg cgccatcgga atttcgcctg ccggtgctga gcccctcgga ccggcgcagt
    15541 ttctcgcgcg cgctgtggag cccgctgctg cccgaggcgc tctggaacag cccgcggcgc
    15601 accgccggtg ccagcgcgca ggtctacctc gacgtgagcg gctcgatgag caaggagatg
    15661 ccgctgatca tcgcgctgct cgcgcgcctc ggctcccaca tccgccgacc gttctgggcc
    15721 ttcagcgacg tggtcgcgcc cgccgtgatc cgcgacgggc agctgcacgc cgacaccacc
    15781 ggcggcacca gcatggcctg cgtgctgcgc cacctggcgc agacgcgccc gcgcgcggcc
    15841 gtggtggtaa cggacggcta tatcgagtcg ctcacacagg cgcaggtgtg cgccgccgcg
    15901 ggcgtgcgcc tgcacgccat cgtgacgcgc gagggctcgc ccgccgcact gcagcgcgcc
    15961 ggcattgggt acacgcagct ggggaggttg ccgtcatgat cggccaatgc tgctggagga
    16021 agcgagcgtg gctgctagag gcgcgcgata gcaccggtcg ggagccacag ggtgagcggc
    16081 gcatccggca agcgctcgaa ttcgaagcgc tcgtaccagc cgcgtgcgat ctcgtccttg
    16141 gcgtcgacga tcactccgat catgccgatc tgctccgaga gccgcaggca gcgctgcaag
    16201 gcgtcggcga gcagcagttc tccgatcccc tggcgctggc accgcacgtc gaccgcaagg
    16261 cgggcaagcc gcgcgacagg caggggaaaa cgcgggaatc gatccgcctc gcgcgccggc
    16321 aggttgtcgc ggcggaccgc ggccatcgcg agggtgtaga agccggcaac gcgcttgccg
    16381 tctgcggcga catagctgcg gctgatattc gccgcagcat gttggcgcgc gtagcggcgc
    16441 aaataatcgt cgagtgccga cacgccgcag ctgaaactgc gccggtcgtg cgttgcaccc
    16501 aagagctcga tcgagagacc ggcccgcgcc ttcacgaacc ggcggtctcc cgctcgcgat
    16561 ggcgtgccat ggcagccgac aaccgtggac gtggcttctc gagcgagtcg agcgccgcca
    16621 cgaaagcctt ccagtccgca gcggtcaacg tgaacgattc ctgctctgcg agcaggtgct
    16681 gcgcgcgctc ctgcgccgcc gcgagcagga acgtgctgac ggacacgccc gcggtcgacg
    16741 cggcgcgcac gatcaactcc ttgatcgcag gggacgtgcg tacatcgatg cgttcactgg
    16801 cttcgctcat gggaggctcc tgtcaccttg gggaggctcg tgaagcctac gccgtcgttt
    16861 tcgcgcatgc aatcgatccg tacaaatgcc ggatcgggga tttccaaggt caacgcctgg
    16921 acccgggagg ttgccgcgat gacgacggtg aattggcgcc gtaggagcgg cccttggccg
    16981 cgacaatcgc gcgcatggcg cgctgctgca gctttcacgc aggaggaact acaaccatga
    17041 tcagggtttc ggaaatggtg ctgcccgggc acccagacaa gttctgcgac caggcggccg
    17101 acgcgatcgt cgcggcctgc tatgccgccg atcccgcggg ctactgccag gtcgagatga
    17161 gctgctggag cgaccaggtg tacctgaccg gcggcatcgc gacggcgcga ccgctcgccg
    17221 agccgctgga cgagatcgtg cgtcgcaccg ggcgcgagct cggctacgtg gcgggcaacg
    17281 cgatcgaggc cgatcgttac gtgatccaca gcagcgtgtg cgacctgcgc accgacgcca
    17341 cgcagtggac gcgccacgtg aacgaccagt gcatctcggt gggctgggcc ggctacgacg
    17401 agaaggtggc gttccttccg ccggagcact acctggcaca cgtgttccgc gaggcactga
    17461 cgcagagctt cgcgaccggg cgcctcgcgc gccagggtcc cgacggcaag ctgatcgtgc
    17521 gcctgcgcga gaacggcgcc gactgggtgc tggagcacgt gctggtcacg gtgcagcagc
    17581 tggatgcctc gtgcatcacc ggggtggcgg catcgctctg cgcggagctg cgcgatgcgt
    17641 acgcggcgct gcaggcgcgc gatgcgcgct gggtggccgg atggcgcgac gtggacgtgc
    17701 tggtgaaccc gaacggcccg ctggtgaacg gcggcagcga cggcgacaac ggccagaccg
    17761 ggcgcaagct cgcggcggac tactacggcc cgcgggtgcc gatcggcggc ggtgcgctct
    17821 cgggcaagga tctctcgcac atcgaccgcg ccgcggccta tgcgttgcgc caggccgcga
    17881 tacacgcggt gcgcggcggc gcgaaggaat gccagctcac cggcgtgtgg gcgccgaatg
    17941 tgggcgtgcc gctcgacgtg atctggcagc tcgacggtcg cggcccgcgc cttgcaaacg
    18001 agtggttcgc gcacggcgcg atcatggagc gcaccgacgt gcgcgcggtg gatcgccacc
    18061 tcgggcgcgg gcggcatttc ttcgacctgg cgctgccgtg gaacggggcc gtgccttgac
    18121 gccgcgtatc ggtcgcgggc atggcccgct cctacggggg gaggtgccgt aacggcagcg
    18181 cggtggttcg tggcgtagga gcgggccatg cccgcgaccg catgtaccgc tagaacgcgc
    18241 tcgcgagctc cgtcagcgct ctcagcgaat cctcgcactc cgcgccctcg atatccgcca
    18301 gcagcagccg tgtcgcgacc aggtgcgtca tgccgaggcg ctcgcggtag cgcgcgatgg
    18361 tatcgcgcac ctggggcggc gaaccgacga tggcccagtc gtccagcccg cgcggcagcg
    18421 agcgcaccag cgcgctggac tgctgctgca tcgccgtcat ccggcgctcc agcagctcgc
    18481 gcaccctggc gatgcgcgcg gtgtcatcgc tgacgaatat cgtgcgcatc accggcacgg
    18541 catcgggcag cggatggccg gcgccggtac aggcggcgcg gtgttgcgcg taattgtcct
    18601 cgagcgtcgc gagcggctcc atcggcgagg cgagatacgg caaccccagc gagccggcct
    18661 gcgccagcgc cttcgggccc agcgccgcga cccacaccgg cgggtgcggc tgctgtaccg
    18721 gcgcgggcat gaggcgcgcg cccgagctgc cgacgggctc gcccgcccac gccgcgcgca
    18781 tgccggccag gcattccgcg aacagctcgc gcttttcgct gcgcttcacc tggaaggcct
    18841 cgaacagctc gggcacgaag ccgcggccca cgccgaggat cacgcgcccg cccgagagct
    18901 ggtccagcac cgccacctgc gcggcggcct gcagtggttg gcgcagcggc agcaggtagc
    18961 tggtggtggc aaggcgaatg cgccgcgtgg acgcggcgac ggcggccagc agcatgagcg
    19021 gttcgggaat ggcgtcggcg ccgaagtggt gttcgggcag ccagaacgat tcgtagccga
    19081 gctcctcggc gagcaccgcc tgacgcgcca gcgcgtcggc gccgcctgcg agcgggaact
    19141 gccacggggt gcagccgagg tgaaggtggt tcatttcaga acgacgatcc gggctgctgc
    19201 aggaacgcga gctcttcggc ggtcgattcg cggcccagga tcgcgttgcg atgcgggtag
    19261 cgcccgaagc gcgcgatgat cgcgtggtgg cggcgctcga aatcgaggct gttctcgagc
    19321 cccggctgat cgaacagccg caacgcgtcc tggtggatct gccccgactc gctgtgcatg
    19381 tacggcatgt agacgaaggc gcgctcgttt ggcgccagct gcgcgtcgaa gccgccgcgc
    19441 accgcttcct gcgccagcgc gagcgccatg ccgtcggcgg cgaaactctc ggcgcggtcg
    19501 cggaacacgt tgcgcgagaa ctggtcgagc acgatcacct cggcgagccg cccgcctgcc
    19561 gagtcgcgcc agccggcgag ctccgcggcc ctggctgccg cgagcgtggc gccgaagcgc
    19621 tgcgcgatgc gtgcgtcgag cgcttcatcc ttgcgccacc actgcgcggg cgtgagctcc
    19681 tcgaaccaga acgccagtac ctgcgaggcg ctggcgatgt cggtgatggt cttcatgctc
    19741 gggttcctcg tctggcgttc gcgcgcgaca aggctaacag gcggacgcgg ggtaacgcat
    19801 ccgtgccgaa atgttgatct gcgccggttg cgcatcgcga acgatcggga atagtctttt
    19861 cgcatgtttc gcacagcgct gatcgaaccg gagggtgaat catgaaaggc aagaggaagg
    19921 cggtgatcga cgtggttcgc gatgcgatcg ccaggggcgc gagcaccgtc gaggaggtgg
    19981 tgaagtcggt ctccgacatg ccgctgaaga agctcgggaa gctcgatccg acgaagcgat
    20041 tgcgcaaggt gcagaacgaa tcgatcggca ccgtcggcga ggccatccgc gacatcaacg
    20101 agcaggtgcg cgcgttcgcg gctgacaagc tggcgcaggc gcgcaaggcc ggcttggtgc
    20161 ccgaggcgaa gccggccggg aaaaagcgcg cggccaaggc ggcgcccgcg aagcgtgccg
    20221 ccagggccgc gccgaaaaag cgcgcggtga aggctgcacc ggcgaaggct gcatcaacca
    20281 aggcggcgcc cgcgaaacag cgtgttgcca aggctgcgcc cgcgaagcag cgagtcgcga
    20341 aggcgtccgt cacggcgagc gctccggcaa agcgcaaggc gtcagtcgcc gcgcccgcgg
    20401 cgtcgtccgt ctcgaagcgt cccgcggttg cacgcaagcg cgtcgccagg cccgcgccgg
    20461 gcgtcaccac gtccgcgccg ggcgtcgcca cgccgccggt tgatgtcatt gcgccgtcgc
    20521 agccgggcga gccgatgacg cagtccgaat gatgcgcccc gctcccgcgg cccgttcacg
    20581 tcgcggcaac accgatgctg gcattgtgca ttcgcccggc gcgcagtggt gaattattga
    20641 tctggcacgg ttgcgcgccg gagcgttggc ggattagggt gaagaacttt caacgaggag
    20701 actgtcccat ggccaagaag aaatcgctga tccacctgat ccaggatgcg atcgacaaag
    20761 gagcgacctc cgccgaagag atccacaagg ctatcgccga caagccgctc aagctgttgc
    20821 tgaaactcga cgcgctgagc ggcccggcga agaagatcaa gcggatccag gacgagtcga
    20881 taggcgccat ttacgacctc atccgcgaga tcaacgagca ggtcgccaag tacgccaccg
    20941 agatgcttgc gcaagcccgc gaggcgagca agggcaagac cgctacctcg gtcaggaagc
    21001 gtgtggcggc cgcggtcaag cgtgtcgaga aggccggcgc cagcgcgaag cggcaggcaa
    21061 aggctgcagc caagcgcgcc cccaaggcgg cggcgaagaa gcgcgcggca gcggttggcg
    21121 ccgtcaagcg aacggtgaag gcggcgacgg cgaaacaagg tcccgcgaag aaggccccgg
    21181 ccaggcgtgg tccgaagccc ggagcccgcg ccagggcgaa agcgtccgcg acggcaccat
    21241 aagccgggcc gcgcgtgaca tcccgccgcc cctcggggtt gcgggacagc tttggctgcc
    21301 cgcggcaatg atgcattcga atccatgggc ggtgacgtga cgaagcggcc ggtggccgtg
    21361 gtgctcggca tcacggccga catggcgttt gccgcggcga acgtgctgct gggcctcgag
    21421 cgcttcccgc cggccgatgg ctacgacgtc gtcatcttcc acaacggagt tcccgcgggg
    21481 gatctcgcgc tgctggcacg gctgcgcgac tgtcatttcg tcgcgtaccg ttgcgcggag
    21541 agcattcgca ggaacattcc cgcccatgcg ctgcgggcgt tctccgagat cgtctacgcc
    21601 aagtacgagt gcttcgagct tctgaaccgc tattcgcgcg tgatctggct cgacaccgac
    21661 atcctgatcc gcggcgatac cgcggagctg ctcgagcgcg gcgcctccgg cgcggccttt
    21721 gcgcgcgagg acaagccgct gcgcttcaac ttcagccggg acatcgaagg cttcgacatg
    21781 gaccgcccgt tcttcaacgc gggtgtgttc gcgctgtccg acgctttgcc gggccgggag
    21841 agcgccaggg actggctgct cgacgccacc gtgcgccatg cggaccgggt gatcatgggc
    21901 gatcagggca tcctgaacct gtggttgcag caccagcgtg tggagccggc ggaactgttg
    21961 cccgaataca acgtgttccg gcatcgcccc ggcgcagccc gggcgcgcgt cgtgcacgcg
    22021 gttggtcatc acaagccgtg gatggatttt gccgatccgc agtggaacgc ggactacgtg
    22081 cgttggctgg cgctgggcgg ctcgccgtgc ccggtatgga agaccgtacg gttcatggcg
    22141 gctcgtcacc cgcacccgct gcgacacccc cgggagatcc cttcgcgtac ggtgcagatc
    22201 ctgtgcttcc tgctgcggcg ccgtcgccag gcgcgcatgg cccgggttgc ctcgcgcaac
    22261 gcctcagccg ggggcgtcct cgacgctgcg tcccactagc tcgagcaccc tggcgatcgc
    22321 ccggcagtaa gcgtcgggaa gcgttgcgcc ccgcgccctg gcggcgccga tcccggcctg
    22381 aatggttgcg cgcaaccggg cagcttcggg cgcatgccgc tcgaagaccg cgcaacggta
    22441 tgccatcagt tgccgctcca accgcgacac cgccgcgccg ggttgcgcga acaggttcgc
    22501 cagcagtacc tcgaactgcg cggccgcgcc cagtcgtgcg cgagccgctg cggccgggat
    22561 gccgctggtg atcgccatga gctcggcgga cgagtcgcgc agctgggcca ggaacgccgc
    22621 catcctccgg tgatagcccg acttgccgcg caaccagtcg ttgagcaggg cgtcggccgt
    22681 cgcgggttcc gcggcggcat cacgcaggta gcaatcgagc gcgcggaact cgaaattcgc
    22741 gacgttcgag cgcgcgggga agaagccgtt gatcgatacg ttgaagtccg cgcagaaggc
    22801 gtcgctgtcc ggcgcgtagg cgttgaggtg gctgcgaaag cgcgcgaact cgtagtcgat
    22861 gaagccgacc tgtgcgccat cgatcaggat gttggcgggc ttgaggtcgt agtgcatcag
    22921 gccgcgggta tccatcgcgt ggaagtgctc gagcagcgcg cccacatggc gggtgtcgag
    22981 cggatggacg cggggatccg gatggttgcc cggaacgtct tccaggaaca ggaaatgcgt
    23041 gtcgccatgg gccacgcggc ccagcagcgc gggcgccccg ggaatgccgg cggcgggcag
    23101 cgatgcaagg atccgggctt cccaggcgaa cgtggtttca ggattggtgc gctgcggccc
    23161 ggggcgggga atcttcagga tcgccgagcg cgagctgcca tcgcaccgga caaggtcgac
    23221 cctgtagcag atgccgttgc ggtcgcgcgc cagttcgccc gactgtcccg ccagcaccag
    23281 ccggatcgcg gctgcgaatg cgcgcggatc gacaggcggg gttgcggcgt cgagcaccac
    23341 gcagtccgtg ttgccggcgc gcgggccgag cgtgctgtcc gcgaacgaac cgtcaggcat
    23401 cgcgggagcc cccggcgctg acgcacagcc tggcggcggc gatgtccctg gcgtaatcga
    23461 tttcctgcca gcggaacggg gcgaaatcga agggcgcgag catcacgtcg ggatgcgcga
    23521 tcaaccgtcg catcacgaag aaatagtagg tgcgcggttc gttgaagccg cgccggatcc
    23581 gctggtccag ggtgtcggcg aacagccggg ccgcttgcgc cgagagcttt atggctcccg
    23641 cgtaggcgcc gctgtagccg gtgaaattga caccaaagcg cgtcacccgg ccgccttcgg
    23701 ccctgatgtt gatctcgcgc gggtccatga cgttgctgtc gtaggtgacc agcgaggctt
    23761 cgggcgcggt gaagagggct tgcgccagtt tgtcggacag cacgaggtcg ccgtgcatga
    23821 acagcagcgc ccggttgaag gactcgcgcg cgaaccacag cgaggcgttg atcccggtga
    23881 tgtcgaaaaa cgggttgatg atgatccggc agccctcgag cagcggcgcg atggtctcgc
    23941 tgcgatagcc cagcagcacg tggatctcgg gcgcggcatc gaactgccgg atctggcgca
    24001 gcagccgcgc gatcaacggc tcgccgtcga tgtcgatcat gcatttgggc aggcccgcca
    24061 gtgcgctgcc catgcgcttg cccacgccgg ccgcgaggat caggtaacgc atgcgctatt
    24121 cgcggcggtc gtgcaacggg tcatgtcagg gcaactggat cgagtagctg aacgtggtct
    24181 gcggtgcagt cgttgcggcg ggccggattg gcgccgtgcc gtcggattcg ccggcgacga
    24241 ttccgaggca ttgcgggtcg agcgcgaatt gcgcttcgac gccgctcgcc tcgatatggg
    24301 cgcgcttggc cgcgatgcgg gctgaaaggg ctgcgcgcag cgcgcgaaag cgctccgcga
    24361 tgctggcaac ggcgcgggga ttgttcgtca tcgccgcctt gaaatgagcg gcggcgtcgg
    24421 gccgttcgcg cagcgcgagt tccccgtcgt ggccgaggtc gaagaactgc agcaggttgc
    24481 aatggttccc cggatccagg cgccaggcgt acatctcgag gccgcgcgag cttgcgagca
    24541 ggacgtaggt gtcgtccgtg gccgcaaaag ccttggtcac gccggtcttg cggttgtcgg
    24601 gctgattggc ggtgaagttc tgcgagaaca cgatgtcctg ctgctgcgtc agcaggttca
    24661 gcccgccata gccgaacccg ccaggctccc cgatgcccag cagcgcaaga caggtcgggg
    24721 cgatgtcgat ggtgctggcg aggttcgcgc ggctggacgg tgtgagcgag ggatcgcgga
    24781 tcgcgcacgg cgtccacgtg atctgggtat acggttccgt cccgtggatc atgccgccct
    24841 tgaagccgtg ggtccagaag tcgtcgccgt ggtcgccgaa catgacgacc gtggtgttgt
    24901 cgagaagccc cttgcgcgcg agtatcccca tcatctcgcc cacggcatgg tcggccacgc
    24961 cgcaggcccg gcggacctgg tccgtgaggc tggcggagaa cggcgccagt gccaggctgt
    25021 gctcgatgtg cgataccagc tcccaggcat agatcgcgaa tggtgcggcg tcggtcagtt
    25081 cgtcgaaacg ccggaacagg gtcggaaaat cgttcgtggc ccataccggt ggcaaatcgg
    25141 cgggccagga ggcgaggcgc gtctcgtcgt tggcgtggaa accgttgagg cagatgaagc
    25201 gcgtgtcgta gccgcgctcc tgcaggatgg aaaacaggtt ccgggtattc ctggccggct
    25261 ggatgccctc gaattcggcg ctggtgtcga actcgagatc gttgccgtgc aggagatacg
    25321 tgatcgccat cagcgtcgat gtcgcggagg aaaagaaatt ctcgaacacc acggcgtcct
    25381 gcatcagccg ccggatgttc gcaaacgcgg agccgaatat cgcgagattg tgccgggtga
    25441 tgctttccag gtgcagtatc agcaggttct tcatggtcgc cagtgtgcca gcgtgccggc
    25501 ttccgggcca gtgccctgga gagccctggt tccatgatgt cctgtggttc agtccggggc
    25561 gcgccgtatt cctgcgccgc cgtaagggtt ggatatgcgt cgggccgatg ggcgcagcaa
    25621 ccggccgctg cctcggggcg aggcgacggc cggttgctgc gagggtcgat cagtcgtcgt
    25681 ccgagtcgtc gtccgaatca tcgtcggagt cgtcgtcgga atcgtcgtcc gagtcatcgt
    25741 cggagtcgtc gtcggaatcg tcgtccgagt ggtcgtggga gtcgtcgttc gaatcgtcat
    25801 ccgaatcgtc atccgaatcg tcgtcgcaca gggggtccac gtcgtcgtcg caatcgtcca
    25861 cggagtcgtc atcgtccagg tcgtcgtcga ggccgtcctt gtcgtcgtcg tcgtcgctga
    25921 atccgtcctt gtcgctgtcg tcgtcgcgga aaccgtcctt gtcgctgtcg tcgtcggaga
    25981 aaccgtcgcc gtcctcgtcg tcacggaagc cgatgacgcg gaagccgttg atcacgtcgt
    26041 cgaagcgcag ctcgaagttg ttgtagaccg tgccgttgat cagcacgcca tcgaggaaga
    26101 tgtggtcgcc ggaaatccgc gcggtggcgc cggacaggtc gacgacttcg ctctggctct
    26161 cggctccggg cgcgttggac acgatgatcg tgcgcgtgag ctcggtcttt tcaccgagct
    26221 ggttcgtggc ctcgatggtg atcgtgtgcg gaccgttgct cagcagcttg gtgttgaagg
    26281 ccgtcgcgaa ccccgagtgc agcggttcgg cgtcgcccgg gaacgccgcc gcaacgtcgg
    26341 cacgctcgcc gcccatgccg atccgcatgg tatggctctc ctggccgtcg atatacagct
    26401 cgaccttcac gaccggctcg acgtccgagg cccagccgta gatctgcgtt atcccgctct
    26461 ggatggaatt cgccgcgggg gcgtcgatgg tgacctgcgg cgcggcatgg agtgccgccg
    26521 gcgccgcgat ggcggcggcg agaagtgcac gcttgaagta tttcatgctc ggtttcctct
    26581 tgtttccgga ttgaccgggg tggcgattcc cgggggcagc gttgcaaaac cggcccgacc
    26641 tggtttgctc gggagcggct ttcatggaca tgacggctgt ggcgcgtggt ttattccaag
    26701 ggtaggggca cgattgcgtg ccatccgtga agttgatcac acgtcgcatc acggcgcagg
    26761 gagtgccggt aaggccccgc gcgcccggcc tgcagctccg ggtgcgcggg gaggtgcgtc
    26821 atttgaaggt ggcggtgacg ctctgggtct tgctcatcgt caccttgcag gagagcgagg
    26881 tgccgctgca cgcgccggac cagccggcga aaaccacctt cctgctggac ggcttggcgg
    26941 tcagggtcac cgtgctgccc gtgctgaagc tcgcggagca cagggtgccg cagctgatgc
    27001 cgctcggctt gctgctgacg ctgcccgagc cgctcttcac cacgctcagc gtgtacttcg
    27061 acggtgccgg tgccggcgcg aaggttgccg tcgcggtcgc gtttgccgcc agcgagaagc
    27121 tgcaggcagc ggtgccggtg caggcgccgc tccagccgct gaacaccatg cccgcatcgg
    27181 gcgtggccgt gaggttgacc gccgtgccga tatcgagcgt cgcggtgcag gcgctgccgc
    27241 agtcgatgcc gttgccgctc accctgccgg tgccgctgcc ggacttggcc gcgctgagcg
    27301 tgaactgctt cttgtcgaag agcgcggtga cgtaggcgtc ggcatttgtc agcgtgaccg
    27361 agcaggcgcc ggtgccggcg caaccggcgc cgctccagcc ggcgaacagg ctgtccgcgg
    27421 cgggagtggc gttcagcgtc gcgctgctgc cggcctcgag cgtgatcgag caggtggcgc
    27481 cgcaatcgat gccgctgccg ctgacgctgc ccgaacccgt gccgctgcga cccacgccga
    27541 ggctgtattg cagcggtgcc ggcgcgcctg cgccaacctg cagcgtgtag gcctggctca
    27601 cgctcatgcc gtcggcatcg gtcaccgcca ccgtgaaggc atagcggccc tcggcggtcg
    27661 gagtgcccga cagcacgccc gggatctcgg cgccggtttc gggatcctgg taagcgccga
    27721 gcaccatgcc gggcggcagc gcgccgttgc cgaggctgat cgcgtagggc gagttgccgc
    27781 cgatcacgac ggtacgcggc gtgtacggcg cggccggctc ggcgtcctgc aggtggtcga
    27841 tgagcccgag cggggcctcg gccgcgaagg cggccatctg ggcgcctatg tagtcgccga
    27901 ccaccgcgag ctggctgcaa tcgaactcgt aggagccgcc gccggcgttg gtgacgttgc
    27961 gcgtgcccac gccgtggatg ccgtccgcgg ccaccttgtc gcacatcgcc tcaccacttt
    28021 cgccgtcgat cgagccgacc ccgatggggt tgtcatcgac gtagcgatag aactcatagc
    28081 ggcgcgtgac gacctcgctg ccgtcgccca tctcgtcggg ggcgagcgcc tcgacgacgt
    28141 cgccgccatc ggcgcggttc tgcaccaggg tccattcgct ctcgacctcg tcgggctccc
    28201 ggttctgcca ttcggcctgg ccgtcgccgt ccaggtcgtc cgagatcaga tcctcgagcg
    28261 cgaccggatc ggggttgtgc gtggtggtct tgatgacctt catccagacg ggctcgccga
    28321 actccttgtt cggcggcacc tcgatctcgg gcgccgggat cgcggccacg acctcggccg
    28381 gttgctcgat gttgccatcg accacgaccg gcgggttgta ggtccacgtg ggcgttgcca
    28441 cggcgaccgg cggcccgagc accaggttgc ccgcgccgtc gtcgatgagc cagttgtaac
    28501 gcagcgccgt gggcgtgccg taatagccca cgccgaagtg ctcgcagccg tcgttgacgc
    28561 tcggattggt gcagctgtgg ccgtcggtgg gtgcgagcgg gccagccggc gtcgccgtgt
    28621 aggcggccca cgtgccgtcg ggattcttcg tgctctcgta gcgcacgaag accttcgggt
    28681 gcgcggggtc gctcaggtcc tcgcggatct tcggtgcgcc gtaatggttc cagtcgtagg
    28741 tgtaggtgat atcggtgctg cggatgtcgt ggatctcgat ctcgaagccg tggcattcct
    28801 gcccggtgtc gttgaccgcg tcgaaattgt tcaggtcgcc gtaggccgtg ctggccgagg
    28861 ctccgtgggc gcccagggcc agcatcgcga tggcgagcgg aaggcgggac agtaaactca
    28921 tgcaaggatc ctctgcgggt aggaaatcgg caggacggcg aacggatttc gcgtcttgcc
    28981 cctgacagaa cggatccacg ctactgttta ttccttaaac cacgctgaaa taccgcggcc
    29041 tgtacaaatg gtcacgcaat cgacggcgcc ctgttgcccc ttcccgcctc gcatggcttg
    29101 ccggataatc gccccgcccc gccacctgga atgaatgcca tgcgtccgtt ggttgtttgc
    29161 gccgtcgtca cctttctcgg agcctgctcc ggcacctcgg gacccatcat cgataccaag
    29221 ggcgtggatc tcgcgcagtt cgagcgcgac aaggccgagt gcgaggcctt tgcaacccag
    29281 gtttccaccg gacaggcggt cggcaggggt gccgccggcg gtgcggcggt gggtgcggcc
    29341 attggcgcca tcaccggtag cgcgggccgt ggcgcgggca tcggggcggt gaccggcggc
    29401 gcggaatccg cgcggatctc cgaacgcgag agggtcaatg tagtgagcaa ctgcctgcgc
    29461 gggcgcggtt accgcgtgct caattgaacc gggcgtgatg cgggtcgcca tccagggcga
    29521 caacaaaaac ccgccacgcg ggcgggtctt tgcatcatga gcggcgccga atctctattt
    29581 cctgttcttg ccgcgccgcg caatgtacag ggcggcgcca atcccgatcg ccagcagggg
    29641 cagggtgcca ggctccggca cggtgccatt ggcgagctgt tccggcgcgt cgaacgtttc
    29701 gagcggctgg ccctgcggag ggaacgattt gcccggcagt gtgggcggga taagcaactc
    29761 aatcggtagc tcgatcggca gttcgatcgg cgggatgaat ggtccgcccg gctgtcccgg
    29821 cagccccccc aggcccggcg gcagcccccc caggcccggc ggca
//
LOCUS       DAORUQ010000016        29736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1108_length_29736_cov_6.530912, whole genome shotgun sequence.
ACCESSION   DAORUQ010000016 DAORUQ010000000
VERSION     DAORUQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29736
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_29736_cov_6.530912"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..842
                     /locus_tag="PLW13_02875"
     CDS             63..842
                     /locus_tag="PLW13_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69340.1"
                     /translation="MRETEHAGRTVVVTGAGGGIGRAAAMLFAQQGAAVVCGDIDLAA
                     AGETARRIAAAGGRALAIAVDVASEASNAALVARAEAEFGTLHIAFLNAGILRRGTVT
                     QTPAAVFDEVMAVNLRGVFLGLQAVAPAIRRAGGGAIVVTASSAGLRSDPELAAYSVA
                     KRGAIALVQSAATEMAPWNIRVNAICPGAVATPMTSAMDSGPGSALALLHPLGRVGTP
                     EDIAELVSFLAGERAGFITGAAYPVDGGLTSVAVPRYRGNA"
     gene            1356..1493
                     /locus_tag="PLW13_02880"
     CDS             1356..1493
                     /locus_tag="PLW13_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69341.1"
                     /translation="MHIDREKDRYTETVTEYESGTVIHHCDEKLSGHTGHGSAKKPKE
                     K"
     gene            1596..2093
                     /locus_tag="PLW13_02885"
     CDS             1596..2093
                     /locus_tag="PLW13_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69342.1"
                     /translation="MANVVIETKTANITMNGTAFMVYGKRYREAAEVWRRTAKSDRRF
                     DPVSYQLLCQSLELHLKSFIWLKDGVGNDVMKKRYGHDIAKLWKDAKAKGISKFAKVT
                     PRRDSAIELLSPYYRKRQFNYLDIDMIFRGFREIDREPHALQTMMQLTKQLGASLREP
                     ILRAS"
     gene            2191..2622
                     /locus_tag="PLW13_02890"
     CDS             2191..2622
                     /locus_tag="PLW13_02890"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HQY69343.1"
                     /translation="MRRLFQFLLVLGVLYAASEQFNLLGRVGGSHDPQAQAIGGAYQQ
                     HASNVQVSGQGEVIRVLADDNNGSRHQRFILRLPTGQTLLVAHNIDLASRIGALREGD
                     RVEFNGEYEWNSQGGVVHWTHHDPQGLHEAGWLKHNGTTYQ"
     gene            2734..3390
                     /locus_tag="PLW13_02895"
     CDS             2734..3390
                     /locus_tag="PLW13_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69344.1"
                     /translation="MGLKDKKVRVIGWTLIPIAALVLCAIFYAAIDRVDTFYVNDSDL
                     HGYAFYLEGREASESYEDSRTKNLPVHAHRIYAGSGTVIGYRKFSNGSLLAVDDEGYE
                     KLTLWLEEPIPEGESIFDLASAETAIVAYAQGGSAWPMHGCFGLLKSGRLQIKKIGTR
                     AEIEVAGEFVPSVRVEERDTSCVSTQLKYSFFAARIEFGELTPWLGMPSDSAYDETYR
                     "
     gene            3556..3795
                     /locus_tag="PLW13_02900"
     CDS             3556..3795
                     /locus_tag="PLW13_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HQY69345.1"
                     /translation="MTPTIFREGGFRFYFFSREEPRMHVHVHCADGEAKFWLEPTIEL
                     AQNLDLNNRQIRTVRSLIEAHEHEIRAAWKRHFGG"
     gene            complement(4081..5184)
                     /locus_tag="PLW13_02905"
     CDS             complement(4081..5184)
                     /locus_tag="PLW13_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQY69346.1"
                     /translation="MTTLSVLDDDALPGRSVRRQEGGNRMSPSSHLQHRRTMRGTLSA
                     TAWLPAFLILLAATLHGNGSAAGERAVTGAQRQVLKVGPAQQYALPSQAARDARDGAL
                     VEIHSGTYEGDVAIWRQNDLTLRGVGERPHLRAAGQAAERKGIWVIKGNRVTVDNLEM
                     SGARVPSHNGAAIRAEGEGLTIRNCHFHHNEMGLLTNPSPAGVVTIEDSEFDRNTTDT
                     ERHGRLGHNIYISQARSFILRNSYVHGARTGHQVKTRARHNEIRDNRIEDGDGWGSLL
                     LDISEGGQAEVINNRFQQSARAGNRTAIGFAGESRDRSSPEHALRVEGNSFINEGGAA
                     TFVRNHTSAPALLRGNRLPARGVTALRGPGSVE"
     gene            complement(5181..6608)
                     /locus_tag="PLW13_02910"
     CDS             complement(5181..6608)
                     /locus_tag="PLW13_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69347.1"
                     /translation="MHRLAGQDAYFLYQETPSALMHTLKISVCKPAASQPTEADFREL
                     VRRSMGCLPVFQYRVVPVPLGLHHPVVVNDGGFDYDMHVHRIAVPAPGARAQLDQVIG
                     EIAAGLLERSRPLWEIWIVEGLEGGRIAYVNKMHHVLADGVAAVNYLGKTLYRDPTDI
                     GKVELPVFEREEQPSAQRLVRDALRDLLRDFLRLPRLLQDSARRRKLIDARNRAARLV
                     PAEPYSPDIPKLRFNRALSNLRNYATAQYSLTDFRTIKNRLGGTVNDVMLGMLAGGLR
                     RYLQTNAELPGRPLVCAIPVSAEQEAAATRTSGNNVAYFHLRLRTDIADIRERYAATR
                     AEAEAGKEVLELLGKHTAHDWMQYIPPLIFSSRRRQDYRMKAADRPGFPLSANVIVSN
                     VPGPRETRYTACGEEVEALYSAGPLTEGTGLNITAWSYAGQMNLGAIACKKALPDLRR
                     LVAEIDAEFAALLALAQQQDTEQGP"
     gene            complement(6642..8177)
                     /locus_tag="PLW13_02915"
     CDS             complement(6642..8177)
                     /locus_tag="PLW13_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY69348.1"
                     /translation="MVREKVLLTGASGSMGFAAFEELWRRRDRFDIVLLLRPSAKNVA
                     MFAPCLEGRRVDGPGTAHYHDGSLKIVWGDAVNHEDIRAACAGVDAVLNPMAFIAPAA
                     DHDPATAEAVNAVAVQSLVKAIRAEPDGAERIRLLHVGSVAQYGDRLPPRHMIRTGDP
                     MQPSVFDFYATTKIRGERAVIESGIRHWASLRQTFITIPSFLTDLLDPITFHLPINTA
                     IEMNTKYDAGRGLVNALDVPRDSDFWGRVYNMAGGPACRVSALGYMKTLFELTGAGDY
                     RRLFERRWFATRNFHCGWFADSHVLNGYLQFWRDDNAAHYEQVRQYMSKLAADMQAAG
                     TPMPGGEDFIRATLEQMVDARTGTRFWRNNGYEARLRAFFGSAASYDAIPDWDVDMPP
                     MPQDVAPRLLDHGYDESKPRLETYDLRQAAEFRGGRLLSPDWDGNGSTVLEWQDAEGR
                     QFSASPNLVLKAGHWSPHEAAPPWDFDRVARRNPFFAQVWHNTHAPDEDNFYPPDCYL
                     DVV"
     gene            complement(8317..8805)
                     /locus_tag="PLW13_02920"
     CDS             complement(8317..8805)
                     /locus_tag="PLW13_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HQY69349.1"
                     /translation="MGQYTPDDDSIIHHELSLDYPDPVIRAMHRAIRVSIRVMALMMA
                     LVIVLGVVDVGYHIYDSLKSPPFLQLSVGEIFQIFGAFMVVLIAIEIFINIRLYLGSN
                     TLPIKMVVATALMAIARKVIVLDLEKTSSGEILAIGVVTLALGITYWLVGNSDSTTTE
                     DQ"
     gene            complement(8864..10330)
                     /locus_tag="PLW13_02925"
     CDS             complement(8864..10330)
                     /locus_tag="PLW13_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69350.1"
                     /translation="MRGSNAAARKDNAKRRQTAPEPQQLPGYRAGSGAGLLLSFADIG
                     EKGVSCVKAPIKPALAVAFATAVLLAGSHPARALNNNVETTRARTDGAYTQVIPYGFY
                     NANTGLAAAAVVLGKGLIQPQVITVANAFVSTSGSYNLFLSNLDMQLPFSRRLFVDQV
                     TYYSDLNEVDNYQDGNPAFPGERAGSNDSDADNYIEATGDDLFAFLNFKYLLPIGHGR
                     ELPPHDFNLTNGMLDPDSAAGGERWNPLKSGRTILEFRPFFRDQDFEDSDTSATIENQ
                     TGGVKVTLEYDNTDWYNNPSTGTRQQLTWARDWGVTDDFPSWTAIQGRHSQYWNLGES
                     GAAQQRVLAFDLWASHSPTWNDRETIDDAEVYSRPPLFEGSTLGGLWRQRGYPTNRFW
                     DRSAINYTLEYRYMPRWNPFPDIPYVNKLFIPWWQWVAFAEVGRVHDEWDLGELHEDM
                     KWTLGGSARLLVYELVIRVDVGVSEEGGEVQMFFNQPF"
     gene            10332..10949
                     /locus_tag="PLW13_02930"
     CDS             10332..10949
                     /locus_tag="PLW13_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007945367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HQY69351.1"
                     /translation="MPIYSYAGYTPVVDPTAFVHPTATLIGDAIVGPRCYVGPGASMR
                     GDFGRVELRAGANLQDNCVMHTFPGRIALVEENGHIGHGAVLHGCRVGSGALIGIGAV
                     IMDNVVIGEHALVAAMSFVKEGMQVPPRVLVAGVPARVVRELTGTEVAWKSNGTLQYQ
                     ELARRSLAEMHECVPLAAEEAGRPVREAGSRPLSETRAETRAEAP"
     gene            10987..12831
                     /locus_tag="PLW13_02935"
     CDS             10987..12831
                     /locus_tag="PLW13_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQY69352.1"
                     /translation="MNRRVAALAMVLALTGCGAKEEQAADVAHDRAAASAEPVVADAE
                     AFIAAAEQQLADSSLYAAKVAWVNATYLNEDTDWLNARALAERTALAVRLASEAARFA
                     ALEGISADTRRKLGILEQMIVLPAPSTPGAAEELATLSTRLKSAYGKGRGTLGGEPKP
                     GVELEALMGTERDPERLKEMWTSWHAVGAPMRQDYVRLVGIANEGARELGFSDLGAMW
                     RSNYDMPPAQFALLTDQLWTEVKPLYEQLHCFTRRQLNERYGDGVQPAMGPIRADLLG
                     NMWAQQWDSIYDVVAPAGAGDIGYDLTELLVKNGYDATRIVKTGEGFFTSLGFAPLPE
                     TFWSRSMITKPADREVICHASAWDIDSKDDLRIKMCTLVNAKDFVTVHHELGHNFYQR
                     AYKQQPYLFLGGANDGFHEAIGDMIALSITPEYLVQIGLLDAAQVPDASRDIGLLLRQ
                     ALEKLPGLPWTVLVDKWRWGVFDGSIPQAEWNKAWNELRLQYMGVVPPVARSEDDFDP
                     GAKYHIPGNVPYARYFLARLLQFQFYQSACEIAGWNGPLHRCSFYGNRDVGAKLDAML
                     ASGRSRPWPEALESFTGSREMHGAALMAYFAPLMGWLEEQNAGHQCGW"
     gene            complement(12880..14676)
                     /gene="recQ"
                     /locus_tag="PLW13_02940"
     CDS             complement(12880..14676)
                     /gene="recQ"
                     /locus_tag="PLW13_02940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HQY69353.1"
                     /translation="MKNALQVLQDVFGYSAFRPPQQEIIDTVVNGGDALVLMPTGGGK
                     SLCYQIPALVRRGCAVVVSPLIALMQDQVDALRQLGVRASFLNSTLSLPAQRAVENAL
                     LGGELDLLYVAPERLFQPQTNALLHQAGIALFAIDEAHCVSQWGHDFRADYLQLERLH
                     REFPGIPRIALTATADQRTRAEIVSRLDLGEARHFIAGFDRPNIRYRIGLKQNAQSQL
                     LRFLREEHPRDAGIVYCLSRKKTEDVARWLCEHGYNALAYHAGMDAAQRAANQSRFLR
                     EDGVIVVATIAFGMGIDKPDVRFVAHLDLPKTIESYYQETGRAGRDGLPADAWLIYGL
                     QDVIKLRQMLESSQGDEEHKRAEQQRLNAMLGLCEITSCRRHALLRYFGEDSPERCGN
                     CDACLEPVDTWDGTEAARMALSAALRTGQRFGVNYLIDVLRGTANERIAQAGHDTLPT
                     FGVGAALDANAWRSVFRQLVARGYLRVDMSEFGTVKLEEKSRALLRGEEHLDLRREEK
                     QKPAARKKTAAAATVAGADQALWDALRECRRELAAEQGVPPYVIFHDATLREMCAARP
                     LDLDAFGQISGVGARKRDKYAEAFLRIIRDHS"
     gene            complement(14713..17136)
                     /locus_tag="PLW13_02945"
     CDS             complement(14713..17136)
                     /locus_tag="PLW13_02945"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQY69354.1"
                     /translation="MKRRKGTGSRILRGSLVTLLLLAAAGAFIRWNGVSRTPGQWWYP
                     FIPALFSDHYTAGPFAMTSNTREDNPVWSRIAGFNRTQARLSYVATRGEPRAQIAWLL
                     ADAEWKDQPVFPSHALSPNKLESSTSKALTAAAVEYDRISRRDLVQATAEGSTLVVGH
                     ARYEALLIADLHVATPELLQNLVTLATQGVPIVWLGDFPARAAGWADHARCDAAVRSQ
                     VERLRGMVVHCASDAQLPGGCGNLRQRAALAPADGSAMPLRMHRRLLGNEQFLLLFNE
                     SDRPVANRFVADHRVRFADLLNPETGDIERLEVVPMATATELRLTVPARRTRVLHLKT
                     GAGRADADAPAPASRWDVEAWHSPPRSMHPFVRWWWPGNAVEQDELRRELNSLHAAGF
                     GGVELQTLTLGFTFDELRKDHDRIYQVGAPAYFDNLLAVFREAERLGMTVDLTLGSGW
                     SSGGPFIERSPSQQLLMASVDARGPASIDIAVPVAQEPWYAARTNAVIPDTIGTFDRD
                     TRLQRLVAARVDGATEPATLSQLRDISGHLHDGRIRWDVPAGHHRIFAFYQNASRHNA
                     AGSAYPGALERAPILDHLDRGGVEEYIEKLGEPWLDALSPFKPDAFFVDSFELIAELP
                     WSAGFARRFEQMHGYAISPWLPLVFRRSGESRYLAALAPQGPAYRSADDRGERVREDY
                     LATREQLFREMFLQPLKDWTTARGVRLRLQAHGGYGDYLDGYQIADIPEAEGLFGGGS
                     FDFLKLASSAAHVAGRPVVASESFIALALDFDALDIEDYHLLAGNAFAAGINRTICHG
                     YAYHYPLQP"
     gene            complement(17220..>17846)
                     /locus_tag="PLW13_02950"
     CDS             complement(17220..>17846)
                     /locus_tag="PLW13_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69355.1"
                     /translation="ALKLMADRGMDGVAINEITEAADVGFGSFYNHFESKEAIHRALT
                     DLVFEEFANALDRALAGVTDPAEIIAASVRHTLYRARRDRTWALFLLRESLSSGMLTR
                     GLGSRLLRDIEKGIGAGRFKAEDPLMAFMGLAGSVLAALSIEVQYGEAADTPLPPALR
                     RAVGLPSPELPERAATTMLRFLGVPPAEARRVARRPLAELETPQTRGD"
     assembly_gap    17847..17946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17947..19591)
                     /locus_tag="PLW13_02955"
     CDS             complement(<17947..19591)
                     /locus_tag="PLW13_02955"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY69356.1"
                     /translation="MSRREMCREIRRRDRMKRSCRPGTLRAVSLALCAVATFAANAAT
                     VGGTPDKAGYSPHAGPAASTRLLWGDTHVHTSYSADAGMTGCRLGPDEAYRFARGEQV
                     LSSTGQPARLRRPYDFLVVADHAENLGLAPMIAASDPLVLQNPTGKRWHDMVSAGKGF
                     EAFADWIQQGSTTGKDPIDSPEMMRAAWEQIIDSAERFNEPGRFTALIGFEWTSTPSG
                     NNLHRNVLLRDDGERARQVVPFSGYDSDDPEKLWQWMQAYEDKTGGRVLAIPHNGNLS
                     NGMMFATTTRDGRPFDRAYAETRARREPLYEVTQPKGTGEAHPLLSPEDEFAGFEVWD
                     KSNLGGNAATTREMLPGNYARTALLNGLALQLRLGTNPFRFGLIGSTDTHTGLSTPGE
                     DNFFGKTVGTEPQAARWEHAAIPAMKPELVTRGWELGASGMVAVWAKDNTRAAIFEAM
                     ERREAYGTTGTRISVRLFAGWELEPGLLERSDFAEQGYARGVPMGGELRRAPTRKSPA
                     FVVQALRDPDSANLDRIQIVKGWVDATGAVHERIFDIAVS"
     gene            19941..20555
                     /locus_tag="PLW13_02960"
     CDS             19941..20555
                     /locus_tag="PLW13_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69357.1"
                     /translation="MALRPASPEGDTRQQILQRAITLFAGAGYAGVSVRDVAAQVGIS
                     AAALYHHFPDKQALYLAAMEHAFARNESGILAALESERSPLERLSGFVTGFVALAAGD
                     QEFSKLVQRELLDGDEARLELLAEHVFRHPHAAIVALARELAPDLDSYMLADSVIGLV
                     LFSFQTAPLRRYLAGRAGPPERPEDIARHVMTLLTRVFARQDAP"
     gene            20555..21637
                     /locus_tag="PLW13_02965"
     CDS             20555..21637
                     /locus_tag="PLW13_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY69358.1"
                     /translation="MPRRIALIPTLCLVLALPAGCSGKKDETPPAPTGTLISVTEATV
                     QDVPVVLKSIGRLESRAAPLVAAEIDGRVLELAVDEGAEIAAGATLAELDATSVTLEV
                     RAAKADLGRIGALLANEERRVERFRTLAARELISREQLEDAEAQLAVLRAQRDAVEAQ
                     LRIVEDQLAKTVIRAPLAGRIQKRMVSVGDFVRRGSPLFEIATSASLRALLPVPEPLA
                     ARLAPGLAVKLGSPLTPDTHATGVITELRPAVGAANRAVWAIVDVENPGAWRPDATVR
                     GEIVLATHADAVVVPGQSVIRRPAGEVVYVIRDGRAEQRVVGTGERVEGLVEIIAGVA
                     AGETVAVEGAAYLSDDAPVRLAGNAP"
     gene            21634..24684
                     /locus_tag="PLW13_02970"
     CDS             21634..24684
                     /locus_tag="PLW13_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY69359.1"
                     /translation="MNLPEISVRRHVLAYMLSGVLVLFGVISFDRIGVDRNPEADFPM
                     ITVVTRLPGANPEIIDSSITNTIEKIVNGVAGIDHVESISAPDRSIVRVKFEMDKDID
                     VAFNEVQVKINQILPQLPEEADPPVVAKVEIGGTPVMWLTLSGDRTLQQLNVYAENEV
                     KKRLETIAGVGEVRIGGERERTIRVELELERMAAFGVTVQDIQAAFRREHVRLPGGFL
                     VGGTGEDLLKADLEFHDLESLAGMIVAHREGAPIRLGDVARIEDGVADFRVLARFNGE
                     PTVGIGVVKVTGANTVEIVNEVKRRLESEIVPQLPAGVEIGIAHDEGELIGQIVAALE
                     EHLVLGTLLTALVVLGFLRNLRATAIVSAAIPVSLLGAVAAIYFLGYTFNTMTLLGLL
                     LLIGVVVDDAIVVLESIFRHGERTGADRLSATIEGAREVTMAVVAATLTLVSIFAPVI
                     FMEGIVARMFEAFAVVVSVGVLASLLVSLTLTPMLCSRHLRLHSGEGAIARRLGGALD
                     ALERHYRRMVALTLRARWSVVLATVLAVASSLWLFGQIGKGFFPEMDEGYFSVTVKVP
                     LGSSIEHAVDRLARVEDVLKRQPGIEAYFSTIGQGQAGQVSEASIVVHLRHWSEREAS
                     QQEIIERVDAELANLAGVEAFPTPASMLGGMRGEPLHFVLVGPELDEVARQAYALRER
                     LLQEGDLGKLDLDLQLALPQLELAVDRDQLAAAGLAGGDVAMALGVLAGGWEVADYND
                     VPGDGERYPIRLKAAEGELARAEDLSRIWLRTASGEMLRLDSVASLRRAVGPAVIARY
                     DLQYAANFYTAPAIPEGVAGPRVLEIARAMMPTGYEVQLAGRAEEFQKTASYMMLTFV
                     TALMLVYMVLASQFNSFVQPLIVMVAQPLAIVGGVFALWATGHTINVFSMIGLVLLVG
                     LVAKNSILLIDLTNQLREQGRSIDAALTEACPIRMRPVLMTSMTIILAMLPAAIGVGA
                     GSDTNGPLAVAVIGGMVSSTLLTLVVVPTVYSLVEHRLERRIRP"
     gene            complement(24794..27235)
                     /locus_tag="PLW13_02975"
     CDS             complement(24794..27235)
                     /locus_tag="PLW13_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY69360.1"
                     /translation="MTFRRAAIAFAIAASQPLHAQVIEEIIVTAQKRAENVMDVPIAI
                     TAYSGEALEQLGTRNLTDIGRFTAGVDMNNDKSLQPTYSIRGVQTNDWTIGSDPAVAV
                     YVDGVYAARGAGAEAAFIDVERVEVLKGPQGTLFGRNATGGAIHIITNRPAFATEGRI
                     KVTGGNYDRRDIEGIYNTALGEDVAMRLTAAVQRRDGYIKDLTGGDDVNEEDKKNVRL
                     ALLWNATANTEVLARIGYEDMDQLSGVLHTTNGAAWEAANPGRRHDDFGDGAWDAPEQ
                     REKRELFSASLEINHQFDDVTFTSITAWRDFETELLEDLDGSNNPEFYFASSNPEQSD
                     FFSQEFRFTGESERLKWTAGATYTSEELEHTTDAQFLVSTFESFALPPVFEQLGMTIT
                     PEEIPAFRAGARTGTNPTLNALNPLLNALYGVNCAGVNCDGIAASFFISSLNGVTLGV
                     SDILDALRPRIAAGYDEPWNETVESQGDYSSWAIYGDTTWSLTDTTNLTVGLRYTYDD
                     KTFDLYTAYQNYLIEPGPGQPGIPFGLAFFNGGQPLLDSTESDNWGAVSGRIVLDHHF
                     TDEVMAYASIANGFKSGGFNSLNFGPDIETSYDSEEVMNYELGVKGTALAGTLQYSSA
                     VFFYDYENLQTLELMGVPIPSYNLRNADAEGKGFEIEAAWMPGEHWLLAGNYSWLDTE
                     FTAYDIIPAAGETEADDLTGEPRADSPEHKYTLSAQYSLPLAQNGELVGRIDYTWADD
                     RIAPSRGEVEDYGLANARLSWFSQDEDWEVALWGTNLADEEVLTVFGNGEAVNSTPAW
                     RIPPRMWGVDLVYNL"
     assembly_gap    27346..27445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27601..28950
                     /gene="gdhA"
                     /locus_tag="PLW13_02980"
     CDS             27601..28950
                     /gene="gdhA"
                     /locus_tag="PLW13_02980"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQY69361.1"
                     /translation="MTTPLDEKLEPIYQEVLRRNPGELEFHQAVREVLECLGPVMVKH
                     PEFVSKKIIERICEPERQIIFRVPWQDDRGEVQINRGFRVEFNSALGPYKGGLRFHPS
                     VYLGIIKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDGEIMRFCQSFMTELYRHL
                     GEYTDVPAGDIGVGGRELGFMFGQYKRITNRYESGVLTGKGLNWGGSLVRTEATGYGA
                     TFFVAEMLRARKDSLEGKTCVVSGSGNVAIYTIEKIHELGGKVVACSDSNGYIYAEDG
                     IKLELVQQIKEVERSRISVYAEREKKARYVAGGNLWDIPCQVAMPSATQNELNGRDAR
                     KLVQNGCIAVGEGANMPTTPDGVRVFLDAKIAYGPGKAANAGGVATSALEMQQNASRD
                     SWSFEHTEKRLHQIMRNIHDLCYETAEEYGTPGNYVNGANIAGFIRVASAMVAMGLI"
     gene            28976..>29736
                     /locus_tag="PLW13_02985"
     CDS             28976..>29736
                     /locus_tag="PLW13_02985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008004555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY69362.1"
                     /translation="MGRLTGKVALITGGARGMGAAAVRIFVREGARVLIADVLEEPGR
                     ALAAQLGDAARFQRLDVSQEGEWEAAMAAAEAMGPLNVLVNNAAILHAAAITQTSLAD
                     YQRVINVNQVGTFLGIRAAIEPMKRAGGGSIVNISSIDGLQSKNGLIAYSASKWAVRG
                     MTKCAAIELGRYGIRVNSVHPGGIDTVMGNPGAMSFEKVSEFYEHHALPRVGLPEEIA
                     NMALFLASDEASYCTGSEYPVDGGWNAGLRNERLPG"
BASE COUNT         4762 a         9491 c        10164 g         5119 t
ORIGIN      
        1 gttgtcatct cgtgggttga gccatcgcgg cgccacaacg gttgtcgacg ggagaattga
       61 agatgcgcga aacggagcat gccggcagga cggtcgtggt gacgggcgcc ggcggcggca
      121 tcggccgtgc cgccgcgatg ctgtttgcgc agcagggcgc ggcggtggtc tgcggcgaca
      181 tcgacctggc agcggcgggc gaaaccgcgc ggcggatcgc ggccgccggg ggccgcgcgc
      241 tggcgatcgc ggtggacgtg gcgagcgaag cgtcgaacgc cgcgctggtc gcgcgcgccg
      301 aggccgagtt cggcacactg cacatcgcct tcctgaacgc cggcatcctg cgccgcggca
      361 cggtgacaca aacgccggcc gcggtgttcg acgaggtgat ggcggtgaac ctgcgtggcg
      421 tgttcctcgg gttgcaggcc gtcgcccccg cgatccggcg cgccggtggc ggggccatcg
      481 tggtcaccgc ctcgagcgcg ggcctgcgca gcgacccgga gctcgcggcc tactcggtgg
      541 ccaagcgcgg cgcgatcgcg ctggtgcaat cggccgcgac cgagatggcg ccctggaata
      601 tccgcgtcaa cgcgatctgc cccggcgccg tggccacgcc gatgacatcg gccatggaca
      661 gcggtcccgg cagcgcgctg gcgctgctgc atccgctcgg gcgcgtgggc accccggaag
      721 atatcgccga gctggtctcg ttcctcgccg gcgagcgcgc tggcttcatc accggcgcgg
      781 cctatccggt cgacggcggg ctgacctcgg tggcggtgcc acggtatcgc ggaaacgcgt
      841 gattctgcgc cccatatggg gcagcttcgg ttgacgggtt tcgcctcgcc tgtcgggtgt
      901 gcttctattg gtctcatggc tcgtaggcgt catgcgcggg ggcatggcgt atttgcgcgg
      961 gacctggtgg tgcataacgt tcgggcaatt gggaaatgct ttgaaatgac aagaggttgc
     1021 cgattgaggg attcgtgttc ggcgacactg atggtttttt atcggacatt tgtgtcgtcg
     1081 aattgtagtt agccttcatg acgaaacaca gcccgacaac ttgcggctct tgtggtgtac
     1141 cgagtgacga gtctctagat acgccagaag cgcgccacgc gtgcgttgct tgcgggagca
     1201 gagcgcgggt atttgctgtc tcaatcgaag aagctattac gctgcgctct ggtctcggct
     1261 tcaagcacaa acgtcctggt cgcaaaaagc ccattgcaga gggattctct cgccccgaaa
     1321 ccgctcgaag cactggggcg gttgtggaac gcaagatgca catcgaccgc gagaaggatc
     1381 gttacaccga gacggttacg gaatacgaga gcggcaccgt gattcatcat tgcgacgaga
     1441 agctttcagg gcatactggt cacggttctg ccaagaagcc taaagagaaa tgacggctaa
     1501 caacacgctg gttccgactc gcaaaggcga agcgcctttg ctcgcggcac agcgtgggcg
     1561 ttaggcatca tcgacccacc gtcgagggcg ttcgagtggc aaatgttgtc atagaaacaa
     1621 agactgcaaa catcacgatg aatggaaccg cattcatggt ttacggaaag agatatcggg
     1681 aagctgcgga ggtctggcgc aggactgcaa aatctgacag gcgctttgat ccagtaagct
     1741 atcagcttct gtgtcagtcg cttgaactcc acctgaagtc gttcatctgg ctcaaggacg
     1801 gtgtaggcaa tgatgtaatg aagaagaggt acgggcatga cattgcaaag ctttggaagg
     1861 acgctaaggc gaaggggata tccaaattcg ccaaggttac accgcgcaga gacagtgcga
     1921 tcgaactcct tagcccgtac tacaggaagc ggcagttcaa ttatcttgac attgacatga
     1981 tatttcgtgg attcagagaa attgatcggg agcctcatgc gttgcagaca atgatgcagc
     2041 tcacgaaaca gcttggagcg tccctgcgcg agccaattct gcgtgctagc tgatgatgcc
     2101 taaccagcgg atccagccga cgtccgtctc gtcgcttcgc tcctcgccgg ccgcggctga
     2161 tccttgtcgt tatgcggcgt gaacgtaggt atgcgtagac tttttcaatt tctgttggtc
     2221 ctgggcgtcc tgtacgccgc ctcggagcag tttaatctac tcggaagggt tggtggctca
     2281 cacgatcccc aagctcaggc aatagggggg gcgtatcaac agcatgcctc caatgtccag
     2341 gtgtctggtc agggagaggt aatacgggtt cttgcggacg acaataatgg aagcagacat
     2401 caaagattca ttctgcggct gcctacgggg cagacacttc ttgtcgctca caatatcgat
     2461 cttgcctcga gaattggtgc gctacgtgaa ggcgatcgcg ttgagttcaa tggcgagtac
     2521 gaatggaact cgcagggtgg tgtggtccat tggactcacc atgatccaca ggggctgcac
     2581 gaggccgggt ggctgaagca caatggaacc acctatcagt gacgagtggc acaagccgcc
     2641 ggataacaaa tcgctccagc cgacttcggt ctcgtcgcga cgctcctcac ccgccccggc
     2701 tgagctcaat cgttatgccc aaaggaaatc gttgtgggcc taaaggacaa gaaggtaaga
     2761 gtcatagggt ggactttgat tccaattgca gcgcttgtcc tttgcgcaat tttttacgcg
     2821 gccatagatc gcgtggacac gttctacgtc aacgattcgg atctccacgg ctatgccttc
     2881 tacctagagg gcagggaggc gagcgagtcc tacgaagaca gcagaaccaa gaatctgcca
     2941 gtgcacgccc atcgcatata tgcggggagt ggaacggtta taggctatcg aaagttctcg
     3001 aatggcagtc tgctggccgt agatgacgag ggatacgaga agttgacgct ttggcttgaa
     3061 gagcctatcc cggagggcga gagcattttt gaccttgcat cggcagaaac ggcgatcgtt
     3121 gcatacgcgc agggcggatc agcgtggcca atgcatggat gcttcgggct actcaaatcg
     3181 ggccgactgc agatcaagaa gattggcaca agagccgaaa tcgaagtggc aggggagttc
     3241 gtgccaagcg ttagggttga ggaacgcgac acatcctgcg taagtacgca gttgaaatac
     3301 tccttctttg cagcaaggat tgaattcggt gagctgacgc catggctggg tatgccatca
     3361 gacagcgcgt acgacgaaac atataggtaa tgagtgcgtg ggggtgtttc ccttcggagt
     3421 cggacgcccc ggattcccgg gtcgtcttcc ccacgtgcgt gggggtgttt ctacccaaac
     3481 gctgcacagt gcgtgcttgg cccgcaaagc gttcctcgat cgttatacct cgaagacgca
     3541 gagatcaacc ttgtcatgac tcctacgatt tttcgtgaag gtggattccg gttctatttt
     3601 ttctctcgcg aggaaccgcg gatgcatgtc cacgttcact gcgcagacgg ggaggcgaag
     3661 ttttggcttg agccgaccat cgaactggcg cagaatttgg acttgaacaa ccgccagatc
     3721 cgaactgtgc ggtcactgat tgaggcacat gagcatgaaa tccgcgccgc gtggaaaagg
     3781 cacttcggcg gctgaggtaa caaacgtatc ggcgcatggc ttctgggtcc tgctcgctga
     3841 cgaagagcta tttgttccgt tcgcagagtt cccctggttt cggaacgcta ctatcggcgc
     3901 catcactcac attgagtggc cttccctcaa ccacctctac tggccggact tggacattga
     3961 tctgtctgta gagtctatcc gtcatccgag ccagttcccg ctggtgtctc gggtagacgc
     4021 ataaccagtc gtttcagccg actttctcgt cgcttcgcgc caccggcctg cccgcgcccc
     4081 tcactcgacg ctacccggtc cccgcagcgc agtgacgccg cgcgcgggaa ggcggttgcc
     4141 cctgagcagg gccggcgcgc tggtgtggtt gcgcacgaag gtcgcggccc cgccttcatt
     4201 gatgaagctg ttgccctcga cccgcagcgc gtgctccggc gacgagcggt cgcgcgactc
     4261 gcctgcaaac ccgatggcgg tccggttccc ggcccgtgcg ctctgctgga accggttgtt
     4321 gatgacctct gcctgcccgc cctcggaaat gtcgagcagg agactccccc agccgtcacc
     4381 gtcctcgatc ctgttgtcgc ggatctcgtt gtgccgcgcg cgcgtcttca cctggtgccc
     4441 cgtgcgcgcg ccgtgcacgt agctgttcct gagaatgaag ctgcgcgcct ggctgatata
     4501 gatgttgtgc ccgagcctgc cgtggcgttc ggtatcggtc gtgttgcggt cgaactcgga
     4561 gtcttcgatc gtcacgacgc cagcggggct cgggttcgtg agcaggccca tttcattgtg
     4621 gtgaaagtgg cagttacgga tggtcaggcc ttctccctcg gcgcgtatgg cggcgccgtt
     4681 gtgcgaggga acccgtgccc cggacatctc caggttgtcc accgtgacgc gattcccctt
     4741 gatgacccag atccccttgc gctcggcggc ctggcccgcg gcgcgcaggt gcgggcgctc
     4801 accaacgccg cgcagggtca ggtcgttctg gcgccagatc gccacgtctc cctcgtacgt
     4861 cccggagtga atctccacca gggcgccgtc gcgcgcgtct cgcgcagcct ggctcgggag
     4921 cgcgtactgc tgcgccggcc cgaccttcag gacctgtcgt tgcgcacccg tgaccgctcg
     4981 ctccccagca gcggagccgt tgccgtgcag tgtcgcggcc agcaggatca agaaagccgg
     5041 cagccacgcc gtggcggaga gggtgccgcg catcgttctg cggtgttgca gatgactgga
     5101 cggtgacatg cggttgcctc cctcctgccg tcgaacgcta cgcccgggca gcgcgtcgtc
     5161 gtcgagtacg gaaagggtgg tcacgggccc tgctccgtgt cctgctgctg cgcgagggcg
     5221 agcagggcgg cgaactcggc gtcgatctcc gcgaccagcc ggcgcaggtc cggcagggcc
     5281 ttcttgcagg cgatggcgcc gaggttcatc tggccggcat agctccaggc cgtgatgttg
     5341 agcccggtgc cctcggtgag cggtccggcc gagtacagcg cctcgacctc ttcgccacag
     5401 gccgtgtagc gggtttcgcg cggcccgggg acgttggaga cgatcacgtt ggccgagagc
     5461 gggaatccgg gtcggtccgc cgccttcatc cggtaatcct ggcgtcggcg cgacgagaag
     5521 atcagcggcg ggatgtactg catccagtcg tgcgcggtgt gcttgcccag cagttccagt
     5581 acttccttgc cggcctcggc ctccgcgcgg gtggcggcat agcgctcgcg gatgtccgcg
     5641 atgtcggtgc gcaggcgcag gtggaaatac gccacgttgt tgcccgaggt gcgcgtggcg
     5701 gcggcttcct gctcggcgct gaccggtatc gcgcacacca gcgggcgccc gggcagttcg
     5761 gcgttcgtct gcaggtagcg tcgcagcccg ccggcgagca tcccgagcat cacgtcgttg
     5821 acggtgccgc cgaggcggtt cttgatggtg cggaaatcgg tcagggagta ctgggcggtg
     5881 gcgtagttgc gcaggttcga cagcgcgcgg ttgaagcgca gcttggggat gtccggcgag
     5941 tagggctctg ccgggacgag gcgtgcggcg cggttgcgcg catcgatcag cttgcgccgc
     6001 cgcgcgctgt cctgcagcag ccgcgggaga cgcaggaaat cgcgcagcag gtcgcgcagc
     6061 gcgtcccgca cgaggcgttg cgccgagggc tgttcctcgc gctcgaatac gggaagctcg
     6121 accttgccga tgtccgtcgg gtcgcgatac agcgtcttgc ccaggtaatt gacggccgcc
     6181 acgccgtcgg cgagcacgtg gtgcatcttg ttcacgtagg cgatgcgacc gccttcaagt
     6241 ccttccacga tccagatctc ccacagcggc cgcgagcgct cgagcaagcc ggccgctatc
     6301 tcgccgatga cctggtcgag ttgtgcgcgc gcgccgggcg ccggcaccgc gatgcggtgc
     6361 acgtgcatgt cgtagtcgaa gccgccgtcg ttcaccacga ccgggtggtg cagccccagc
     6421 ggcacgggta cgacgcggta ctggaacacg ggcaggcagc ccatggagcg gcgcaccagt
     6481 tcgcggaaat ccgcctcggt cggctggctg gcggcgggct tgcacaccga gatcttcagc
     6541 gtgtgcatca acgccgaagg tgtttcctgg tacaggaagt acgcgtcttg cccggccagc
     6601 ctgtgcatcg cccgtctccc tcgcaggtgc cgccgcacgc ctcagacaac gtcgaggtag
     6661 cagtcgggcg ggtagaaatt gtcctcgtcg ggcgcgtggg tgttgtgcca cacctgcgcg
     6721 aagaaggggt tgcggcgcgc gacgcggtcg aagtcccagg gcggtgcggc ctcgtgcggc
     6781 gaccagtgcc ccgccttcag caccaggttg ggtgaagcgc tgaactgcct gccctcggcg
     6841 tcctgccact cgagcaccgt gctcccgttg ccgtcccagt ccggcgacag caaccgcccg
     6901 ccgcggaatt ccgcggcctg gcgcaggtcg tacgtctcga gccggggttt gctctcgtcg
     6961 taaccgtgat cgagcagccg cggcgccacg tcctgcggca tcggcggcat gtcgacgtcc
     7021 cagtcgggta tcgcgtcgta gctcgcggcc gagccgaaga atgcgcgcag gcgcgcctcg
     7081 tagccgttgt tgcgccagaa acgcgtgccg gtgcgcgcgt cgaccatctg ctcgagcgtc
     7141 gcgcgtatga agtcctcccc gccgggcatc ggggtgccgg cggcctgcat gtcggccgcg
     7201 agcttgctca tgtactggcg cacctgctcg tagtgcgcgg cgttgtcgtc gcgccagaat
     7261 tgcaggtagc cgttcagcac gtggctgtcg gcgaaccatc cgcagtggaa gttgcgcgtc
     7321 gcgaaccagc gccgctcgaa gagcctgcgg tagtcgcccg ctcccgttag ctcgaacagg
     7381 gtcttcatgt acccgagcgc gctgacgcgg cacgccggtc cgccggccat gttgtatacc
     7441 cggccccaga agtccgagtc acgcggcaca tcgagcgcgt tgaccagccc gcggcccgcg
     7501 tcgtacttcg tgttcatctc gatcgcggtg ttgatcggca ggtggaacgt gatcggatcc
     7561 agcaggtccg tcaggaaaga gggaatcgtg atgaaggtct ggcgcagcga cgcccagtgc
     7621 cggatgccgc tctcgatcac ggcgcgctcg ccgcggatct tggtggtggc atagaagtcg
     7681 aacacggagg gctgcatcgg atcgccggtg cggatcatgt gtcgcggcgg caggcggtcg
     7741 ccgtactgtg cgaccgagcc cacgtgcagc agccgtatgc gctctgcgcc gtcgggctcg
     7801 gcccggatcg ccttcaccag cgactgcacc gcgaccgcgt tcaccgcctc ggcggtggcc
     7861 ggatcgtggt ctgccgccgg cgcgatgaac gccatcgggt tgagcaccgc atcgacgccc
     7921 gcgcaggcgg cgcggatgtc ctcgtggttg acggcgtcgc cccagacgat tttcaggctg
     7981 ccgtcgtggt aatgcgcggt gccaggcccg tcgactcgcc gcccctcgag gcagggcgca
     8041 aacatcgcga cgttcttcgc cgagggtctg agcaacagga cgatgtcgaa gcgatcccgc
     8101 cgacgccaca gctcctcgaa tgccgcgaag cccatggacc ccgacgcccc cgtcaacaag
     8161 accttttcgc gcaccatcgc cgcctccccg ctgcctcgct tctccggtcg ctgcgccgag
     8221 aatcgctcac ggcgcgcctg ctgtcatcac ccggaccgac caggcgcaac gcaccgggcg
     8281 cgcctcgccg tcacgatgcg ggggcgatcc gggacgctat tgatcctcgg tcgtcgtgct
     8341 gtcgctgttg ccgacgagcc agtaggtgat gcccagtgca agcgtgacca cgccgatcgc
     8401 caggatctcg cccgaagacg tcttctcgag atcgagcacg atgaccttgc gcgcaatggc
     8461 cattagcgcc gtagccacca ccatcttgat cggcagcgta ttggacccga gatacagcct
     8521 gatattgatg aatatctcga tcgcgatcag cacgaccatg aatgcgccga aaatctggaa
     8581 gatctccccc acgctcaact gcaggaaggg gggggatttc aggctgtcgt atatgtgata
     8641 gccaacgtcc acgacgccaa ggacgatgac gagcgccatc atcagtgcca ttacccttat
     8701 ggacacccgg atggcgcggt gcatggcgcg gatgaccgga tcggggtaat cgagcgacaa
     8761 ttcgtggtga atgatcgaat cgtcgtccgg cgtgtattgc cccatgtgag ctactccttg
     8821 gccggggatg ctcccccggg tagtaaaaag cgcgaatgct gattcagaac ggttgattga
     8881 agaacatctg cacctcgccc ccttcctcgc tgacgcccac gtcgacgcga atgacgagtt
     8941 cgtagaccag cagtcgcgcc gatccgccca gcgtccactt catgtcctcg tggagctcac
     9001 cgagatccca ctcgtcgtgc acccgaccca cctccgcgaa cgccacccac tgccaccagg
     9061 gaatgaacag cttgttgaca taggggatgt cgggaaacgg gttccaccgc ggcatgtagc
     9121 ggtactcgag cgtgtagttg atcgccgagc ggtcccagaa acggttggtg ggatagccgc
     9181 gttgacgcca gaggccgccc agggtggagc cctcgaacag cggcgggcgg ctatacacct
     9241 cggcgtcgtc gatcgtctcg cggtcgttcc aggtcgggga gtgggaagcc cacaggtcga
     9301 aagccagcac ccgctgctgc gccgcgccgc tctcgcccag gttccagtac tggctgtgcc
     9361 tgccctgtat ggcggtccag ctggggaaat cgtcggtgac gccccagtcc ctggcccacg
     9421 tcagttgttg cctggtgccc gtggaggggt tgttgtacca atccgtgttg tcgtattcga
     9481 gggtgacctt gaccccgccg gtctggttct cgatggtcgc gctggtatcg gagtcctcga
     9541 agtcctggtc gcggaaaaac ggtcggaatt cgaggatggt gcggcctgac ttcagtggat
     9601 tccagcgctc gccccccgcc gccgaatccg ggtcgagcat gccgttggtg aggttgaaat
     9661 cgtgcggcgg caattccctg ccgtggccga tcggcagcag gtacttgaag ttgaggaacg
     9721 cgaacaggtc atcgcccgtc gcctcgatgt aattgtcggc gtccgaatcg ttcgagcccg
     9781 cccgctcgcc gggaaacgcc ggattgccgt cctggtagtt gtcgacctcg ttcaggtccg
     9841 agtagtacgt gacctgatcg acgaacagac gccgcgagaa cggcagctgc atgtcgaggt
     9901 tggacaggaa gaggttgtag gaaccggacg tgctgacgaa ggcgttggcg acggtgatca
     9961 cctgcggctg gatcaagccc ttgcccagta ccacggcggc agcggccaat cccgtattcg
    10021 cgttgtagaa accgtacggg atcacctggg tgtaggcgcc gtcggttcgc gctcgcgtcg
    10081 tctcgacatt gttgttgagc gcccgggcgg gatgcgaccc ggccagcaag actgctgtcg
    10141 cgaaagcgac ggcgagagcc ggctttattg gcgccttcac gcaggaaact cccttctccc
    10201 cgatatcggc aaaacttaac agcaaaccgg ccccggagcc agcacggtat cccggcagtt
    10261 gctgcggttc aggcgcggtt tgacgccgtt tggcgttatc cttgcgcgct gcagcattgg
    10321 agccacgcaa gatgccgata tattcctacg ccggttacac accggtcgtc gatcccaccg
    10381 ccttcgtgca ccccaccgcc acgctgatcg gcgatgcgat cgtgggtccg cgatgctacg
    10441 tagggcccgg agcgagcatg cgcggcgact tcggccgcgt cgaactgcgc gccggcgcca
    10501 acctgcagga caactgcgtg atgcacacct ttccggggcg catcgcgctg gtcgaggaga
    10561 acgggcacat cgggcacggc gcggtgctgc acggctgccg cgtcggcagc ggtgcgctga
    10621 tcggcattgg cgcggtgatc atggacaacg tggtgatcgg cgagcacgcg ctggtggcgg
    10681 cgatgagctt cgtgaaggag ggcatgcagg tgccgccgcg cgtgctggtc gccggcgtgc
    10741 cggcgcgcgt ggtgcgcgag ctcaccggga ccgaggtcgc ctggaagtcc aacggcacgt
    10801 tgcagtacca ggaactggcg cgccgttcgc tcgccgaaat gcacgaatgc gtgccgctgg
    10861 cggccgagga agcgggccgg ccggtgcgcg aggccggctc gaggccgctg tccgaaacgc
    10921 gtgccgaaac gcgcgccgaa gcgccgtgaa gtgcagtttc caggcaatgg ttcgcgggag
    10981 tggagcatga acagacgggt cgcggcattg gcgatggtgc ttgcgctgac gggctgcggc
    11041 gcgaaggaag agcaggccgc ggacgtggcc catgaccggg cggcggcgag cgccgagcct
    11101 gtggtagccg acgcggaggc cttcattgcc gcggcggagc agcaactcgc ggactcttcc
    11161 ctctacgcgg cgaaggtcgc atgggtcaat gcgacctatc tgaacgagga caccgactgg
    11221 ctgaacgcgc gggcgctggc cgagcgcacg gcgctcgcgg tgcgcctggc gagcgaggcc
    11281 gcgcgcttcg ccgcgctcga ggggatttcc gccgacacgc gccgcaagct cgggatcctc
    11341 gagcagatga tcgtgctgcc ggcgccttcc acgccgggcg ctgccgagga actcgcgacc
    11401 ctttctacgc gcctgaaatc cgcctacggc aagggcaggg gcacgctggg cggtgaaccg
    11461 aagcccggcg tcgagctcga ggcgctgatg ggcaccgagc gcgatcccga gcggctgaag
    11521 gagatgtgga cctcgtggca cgcggtgggc gcaccgatgc gccaggatta cgtgcgcctg
    11581 gtcgggatcg ccaacgaggg cgcccgtgag ctgggcttca gcgatctcgg cgcgatgtgg
    11641 cgctcgaact acgacatgcc gccggcgcag ttcgcactgc tcaccgacca gctctggacc
    11701 gaggtcaagc cgctctacga gcagcttcac tgcttcacgc ggcgccagct taacgaaagg
    11761 tacggcgacg gggtgcagcc cgccatgggc ccgatccgcg ccgacctgct cggcaacatg
    11821 tgggcgcagc agtgggactc gatctacgac gtcgtcgcgc cagccggcgc cggcgacatc
    11881 ggttacgacc tcaccgagct gctggtgaag aacggctacg acgcgacgag gatcgtgaag
    11941 accggcgagg gcttcttcac ctcgctcggg ttcgcaccat tgccggaaac cttctggtcg
    12001 cgctcgatga tcacgaagcc ggcggaccgc gaggtgatat gccacgcctc ggcctgggat
    12061 atcgacagca aggacgacct gcgcatcaag atgtgcacgc tggtcaacgc gaaggacttc
    12121 gtgaccgtgc accacgagct cggccacaac ttctaccagc gcgcctacaa gcagcagccc
    12181 tacctgtttc tcgggggtgc caacgacggc ttccacgagg cgatcggcga catgatcgcg
    12241 ctgtcgataa cgcccgagta cctggtgcag atagggttgc tcgacgccgc tcaggtgccc
    12301 gacgcctcgc gcgacatcgg cctgctgctg cgccaggcgc tggagaagct gccgggcttg
    12361 ccgtggacgg tgctggtcga caagtggcgc tggggcgtgt tcgacggcag catcccgcag
    12421 gccgagtgga acaaggcgtg gaacgaactg cgcctgcagt acatgggcgt ggtgccgccg
    12481 gtagcgcgca gcgaggacga tttcgatccg ggggcgaaat atcacatccc gggcaacgtg
    12541 ccctacgcgc gctacttcct ggcgcgcctg ctgcagttcc agttctacca gagcgcctgc
    12601 gagatcgcgg gctggaacgg gccgctgcac cgctgctcgt tctacggcaa ccgggacgtg
    12661 ggcgcaaagc tcgacgcgat gctcgcctcg ggacgttcgc gtccctggcc cgaggcgctg
    12721 gagagcttca ccggcagccg cgagatgcac ggcgcggcgc tcatggccta cttcgcgccg
    12781 ctgatgggct ggctcgagga gcagaacgct ggccaccagt gcggctggtg acggggggcg
    12841 caccgtcggc atgaatgccg acccacaatc gccccatgct catgaatgat cgcggatgat
    12901 gcgcaggaac gcctcggcgt acttgtcgcg cttgcgcgct cccacgccgc tgatctggcc
    12961 gaaggcgtcg aggtcgagcg gccgcgccgc acacatctcg cgcaaggtgg cgtcgtggaa
    13021 aatcacgtac ggcggcacgc cctgttcggc ggccagctcg cgccggcatt cgcgcagcgc
    13081 gtcccacagc gcctggtccg ccccggcgac cgtggccgcg gccgcggtct tcttgcgcgc
    13141 tgcgggcttc tgcttttcct cgcgcctgag atccagatgt tcctcgccgc gcagcagcgc
    13201 gcgtgatttc tcctcgagct tcacggtgcc gaactcgctc atatcgacac gcaggtagcc
    13261 gcgcgccacc agctggcgga acaccgagcg ccaggcgttg gcatcgagtg cggcgccgac
    13321 gccgaaggtc ggcagtgtgt cgtggccggc ctgtgcgatg cgctcgttcg ccgtgccgcg
    13381 cagcacgtcg atcaggtagt tcaccccgaa gcgctgcccg gtgcgcagcg ccgcgctgag
    13441 cgccatgcgc gccgcttcgg tgccgtccca ggtatccacg ggctccaggc aggcatcgca
    13501 gttgccgcaa cgctcgggcg aatcttcgcc gaagtagcgc agcagcgcgt ggcgccggca
    13561 actcgtgatc tcgcacagcc ccagcatcgc gttgagccgt tgctgctccg cgcgcttgtg
    13621 ctcctcgtcg ccctgcgagc tctcgagcat ctggcgcagc ttgatcacgt cctgcaggcc
    13681 gtagatcagc cacgcgtcgg cgggcagccc gtcacggccg gcgcggccgg tttcctggta
    13741 ataggactcg atggtcttcg gcaggtcgag gtgcgcgacg aagcgcacgt cgggcttgtc
    13801 gatgcccatg ccgaaggcga tcgtcgccac cacgatcacg ccgtcctcgc gcaggaagcg
    13861 cgactggttc gccgcgcgct gcgcggcgtc catgccggcg tggtaggcga gcgcgttgta
    13921 gccgtgctcg cagagccagc gcgccacgtc ctcggtcttc ttgcgcgaca ggcagtacac
    13981 gatgccggcg tcgcgcggat gctcctcgcg caggaaacgc agcaactgtg actgcgcgtt
    14041 ctgcttcagg ccgatgcgat agcggatgtt cgggcggtcg aagccggcga tgaagtgccg
    14101 cgcctcgccg aggtcaagcc tcgatacgat ctcggcgcgc gtgcgctggt cggcggtcgc
    14161 ggtcagcgcg atgcgcggga tgccggggaa ttcccggtgc agccgttcga gctgcaggta
    14221 gtcggcgcga aaatcgtggc cccactgcga gacgcaatgc gcttcgtcga tcgcgaacag
    14281 cgcgatgcct gcctggtgca acagcgcgtt ggtctgcggc tggaacagcc gctcgggcgc
    14341 gacgtagagc aggtcgagct cgcccccgag cagcgcgttc tcgacggcgc gctgcgccgg
    14401 gaggctcagc gtggaattga ggaaactcgc gcgcacgccg agctggcgca gcgcatcgac
    14461 ctggtcctgc atcagcgcga tcagcggcga gaccacgacc gcgcaccccc ggcgcaccag
    14521 cgcgggaatc tggtagcaca gcgatttgcc gccgccggtg ggcatcagca cgagcgcatc
    14581 gccgccgttc accacggtgt cgatgatctc ctgttgcggg gggcggaaag cggagtaacc
    14641 gaagacgtcc tgcaggacct ggagcgcgtt tttcatcggc ggcattatag gctcgcgcga
    14701 cgcgtgcgag catcacggct gcaacggata gtgataggcg tagccgtggc agatcgtgcg
    14761 attgattccg gcggcaaagg cgttgcccgc cagcaggtgg tagtcctcga tgtcgagcgc
    14821 gtcgaagtcg agcgccagcg cgatgaatga ttccgaagcg accacgggac gcccggcaac
    14881 gtgggccgcc gatgaggcca gcttcaggaa atcgaagctg ccgccgccaa acagaccctc
    14941 ggcctcgggt atgtcggcga tctggtagcc gtcgagatag tcgccatagc cgccgtgcgc
    15001 ctgcaggcgc agcctgacgc ccctggctgt ggtccagtcc ttcagcggtt gcaggaacat
    15061 ctcgcggaac aactgttcgc gggtcgccag gtagtcctcg cgcacgcgct cgccccgatc
    15121 gtcggccgat cgatacgccg gtccctgtgg cgccagcgcc gcgagatacc tggattcgcc
    15181 ggatcgccgg aacaccagcg gcagccaggg cgagatagcg tagccgtgca tttgctcgaa
    15241 ccggcgcgca aaacccgccg accagggcaa ctcggcgatc aactcgaagc tgtccacgaa
    15301 gaaggcgtcg ggcttgaacg gggacagggc atccagccat ggctcgccca gcttttcgat
    15361 gtactcctcg acgccgccgc ggtccaggtg atcgagtatc ggcgcgcgct cgagtgcgcc
    15421 cggatacgcg gagccggcgg cattgtgcct gctcgcgttc tggtagaacg cgaatatccg
    15481 gtgatgcccc gcgggaacgt cccagcggat ccggccgtcg tggaggtggc cactgatgtc
    15541 gcgcagttgc gagagcgtgg ccggttccgt ggcgccgtcc acccgcgcgg ccaccagccg
    15601 ctgcagccgt gtgtcgcggt cgaatgtccc gatcgtgtcg ggtatgacag cgttggtcct
    15661 cgcagcgtac cagggctcct gtgcaaccgg cacggcaata tcgatcgatg cggggccccg
    15721 tgcatccacc gacgccatga gcagttgttg cgaaggtgag cgctcgatga acggaccacc
    15781 gctcgaccag ccgctgccca gggtcaggtc caccgtcatg ccgaggcgtt cggcctcccg
    15841 gaacacggcc agcagattgt cgaaatacgc gggcgctccg acctggtaga tgcggtcatg
    15901 gtccttgcgc aactcatcga aggtgaagcc gagcgtcagg gtctgcagtt ccacgccgcc
    15961 gaatccggcc gcgtgcaggc tgttcagttc gcggcgcaac tcgtcctgct cgaccgcgtt
    16021 gccaggccac caccagcgca cgaaggggtg catcgatcgc gggggagagt gccaggcctc
    16081 gacgtcccac ctgctggcgg gcgccggtgc atcggcgtcg gcccgcccgg cgcccgtctt
    16141 caggtgcagt acccgcgtgc gcctggcggg caccgtgagc cgcagttcgg tcgccgtcgc
    16201 catcggcacc acctcgaggc gctcgatgtc gcccgtttcc gggttcagca agtcggcgaa
    16261 ccgcacgcgg tggtccgcga caaagcggtt tgcgacgggc cggtcggatt cgttgaacag
    16321 caacaggaat tgctcgttgc cgagcaggcg ccggtgcatg cgcagcggca tcgcgctgcc
    16381 gtcagccggc gcgagggctg cgcgctggcg caggttgccg cagccgccgg gcagctgtgc
    16441 atccgacgcg cagtgcacaa ccatcccccg cagccgctcg acctgggagc gcaccgccgc
    16501 gtcgcaccgt gcgtgatccg cccagccggc agcgcgcgcc gggaagtcgc ccagccatac
    16561 gatcggcaca ccctgcgtcg ccagcgtgac caggttttgc agcagttcgg gtgtcgcgac
    16621 gtgaaggtcc gctatcagca gcgcctcgta gcgggcgtgg ccgaccacga gcgtcgatcc
    16681 ctcggcggtg gcctggacga ggtcgcgccg gctgatccgg tcgtactcca cggcggcggc
    16741 ggtgagcgcc ttgctggtgg acgattcgag cttgttggga ctgagcgcgt gcgacgggaa
    16801 taccggctgg tctttccatt cggcgtccgc cagcagccac gcgatctgcg cgcgcggttc
    16861 gccgcgcgtc gccacgtagc tcagccgcgc ctgcgtgcgg ttgaatccgg cgatccggct
    16921 ccagaccggg ttgtcctcgc gcgtattgct ggtcatcgcg aacggaccgg cggtgtagtg
    16981 gtcgctgaac agcgcgggaa tgaacgggta ccaccactgc ccgggcgtcc gcgacacgcc
    17041 attccagcgg atgaatgccc cggcagccgc caacagcagc agcgtgacga gcgagccgcg
    17101 gagaatcctc gatccagtgc cttttctgcg tttcacgttt tcagctcact gcggcgtacc
    17161 cgtggatcgc gatccgcgag gtggcatcga tagcacgctg gacctgcgcg gcggcggtct
    17221 cagtcgcccc ttgtctgcgg cgtttccagt tcggccagtg gcctgcgggc gacgcgccgc
    17281 gcctccgccg gcggcacccc gaggaatcgc agcatggtcg ttgcggcgcg ttccgggagc
    17341 tcggggctcg gcaggccgac cgcgcggcgc agcgccgggg gcaggggggt gtcggcagct
    17401 tcgccgtact gcacctcgat cgacagcgcg gcgagtacgc tgcctgcgag tcccatgaag
    17461 gccatcagcg gatcctccgc cttgaaacgc ccggcgccga ttcccttttc gatgtcgcgc
    17521 agcagccgcg atcccagtcc gcgggtgagc atgcccgagg acagggactc gcgcagcagg
    17581 aacagggccc aggtccggtc gcgtcgcgcc cggtagagcg tgtgccgcac cgatgcggca
    17641 atgatctcgg cgggatccgt gacccccgcg agcgcgcggt ccagcgcatt cgcgaactcc
    17701 tcgaacacca ggtcggtcag ggccctgtgg atggcctcct tcgattcgaa gtggttgtag
    17761 aaagagccga aacccacgtc ggcggcctcg gtgatctcgt tgatcgcgac cccgtccatg
    17821 ccgcgatcgg ccatcagctt cagggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnccga caccgcgatg tcgaatatac gctcgtgcac ggcgcccgtg gcgtcgaccc
    18001 agcccttcac gatctggatg cggtcgaggt tcgcgctgtc ggggtcgcgc agtgcctgca
    18061 ccacgaaggc gggggatttg cgtgtcgggg cgcggcgcag ctcgccgccc atcggcacgc
    18121 cgcgcgcgta gccctgctcc gcgaagtcgg aacgctccag tagccccggc tcgagctccc
    18181 agccggcgaa caggcgcacc gagatccggg tgccggtggt gccgtaggct tcacgccgct
    18241 ccatcgcctc gaagatcgca gcgcgggtgt tgtccttcgc ccacacggcc accatgcccg
    18301 aggcgccgag ttcccagccg cgcgtgacca gctccggttt catggccggg attgccgcgt
    18361 gctcccagcg tgccgcctga ggttcggtgc cgacggtctt gccgaagaaa ttgtcctcgc
    18421 ccggggtgga gagaccggtg tgcgtgtcgg tgctgccgat cagcccgaag cggaacgggt
    18481 tggtgcccag gcgcagctgg agggccagcc cgttcagcag cgcggttcgg gcgtagttgc
    18541 cgggcagcat ctcgcgggtc gtcgccgcgt tgccgccgag attcgacttg tcccacacct
    18601 cgaagccggc gaactcgtcc tccggcgaca gcagggggtg cgcctcaccg gtgcccttgg
    18661 gctgcgtgac ctcgtacagc ggttcgcggc gcgcgcgggt ctcggcgtag gcacgatcga
    18721 acggacggcc gtcgcgtgtg gtcgtggcga acatcatgcc gttgctcagg ttgccgttgt
    18781 gcgggatggc cagcacgcga ccgccggtct tgtcctcgta ggcctgcatc cactgccaga
    18841 gcttctcggg gtcgtcgctg tcgtagccgg aaaagggcac gacctggcgc gcacgttcgc
    18901 cgtcgtcgcg caacagcacg ttgcggtgca ggttgttgcc cgagggcgtc gacgtccact
    18961 cgaagccgat cagggcggtg aaacgccccg gctcgttgaa gcgttccgcc gagtcgatga
    19021 tctgctccca tgcggcgcgc atcatctcgg ggctgtcgat gggatccttg ccggtggtgc
    19081 tgccctgctg gatccagtcc gcgaacgcct cgaagccctt gcccgcggag accatgtcgt
    19141 gccagcgctt gccggtggga ttctgcagta ccagcgggtc ggaggcggcg atcatcggcg
    19201 ccaggccgag gttctcggcg tggtccgcaa ccaccaggaa atcgtagggg cggcgcagcc
    19261 tcgccggctg gccggtgctg ctcagcacct gctcgccgcg cgcgaagcgg taggcctcgt
    19321 cgggtccgag gcggcagccg gtcatgccgg cgtcggcgga ataggaggtg tgcacgtgcg
    19381 tgtcgcccca cagcagtcgc gtgctcgcag cggggccggc gtggggcgaa tagcccgcct
    19441 tgtcgggtgt cccgcctaca gttgccgcgt tggcagcaaa tgtcgcgacc gcgcacaggg
    19501 ccagcgacac ggcacgcagc gtgcccggtc ggcaggaacg cttcatcctg tcgcggcgac
    19561 gaatctcgcg gcacatttca cggcgactca tccgcacctc ccgattttcc gctgtgaacc
    19621 ttgcttgcgc gacttgtccg ccgaagccga caggtgtccg ccgtcgcgcc gcgcatgcgc
    19681 cgcgacgata ccggattggg ggaagggtga caataatatc agttacgctc aatcaatcat
    19741 ttgaggcgta ggtcgccatt catggtgact cgcgcctgcg gggatgcggc gggttcgagg
    19801 gtaccgaatc cgtgtcgggc tgaagcccga cctacagccg acccgcatct ggaccggaat
    19861 gatcgcgcag ttttgcgcgt tgacaggtgg tgcggggtgg gcctaggctt tccttaacaa
    19921 acgttaggta aggagtcggc atggccctgc gtcccgcctc acccgagggc gacacgcggc
    19981 agcagatact gcaacgcgcg atcacgctgt tcgcgggcgc tggctacgcg ggcgtttcgg
    20041 tgcgcgacgt cgccgcccag gtcggcatca gcgcggctgc cctgtaccat cactttcccg
    20101 acaagcaggc gctctatctc gccgcgatgg agcacgcctt tgcgcgcaac gagtcgggca
    20161 tcctcgcggc gctggagagc gagcgctcac cgctcgagcg cttgagcggt ttcgtcacgg
    20221 ggtttgtggc cctggcggcc ggtgaccagg agttcagcaa gctggtgcag cgcgaactgc
    20281 tcgacggcga cgaagcgcgt ctcgaattgc tggcggagca cgtcttccgc catccgcacg
    20341 cggccatcgt tgcgctggcg cgcgaactgg ccccggacct ggattcctac atgctggccg
    20401 attccgtgat cgggctggtg ctgttcagtt tccagaccgc tccgctgcgg cgctacctcg
    20461 cgggacgcgc cgggccgccc gagcgccccg aagatatcgc ccgccatgtc atgaccctgc
    20521 tcacgcgggt ctttgctcgc caggatgccc cgtgatgccg cgtcgaattg ccctgatccc
    20581 cacgctgtgc ctcgtcctcg cgctgcctgc cggctgctcc ggcaagaagg atgaaacgcc
    20641 gcctgcgccc accggcacgc tgatcagcgt gaccgaggcg accgtgcagg acgtgccggt
    20701 ggtgctgaaa tcgatcgggc gcctggagag ccgcgccgcg ccgctggtgg cggccgagat
    20761 cgacggtcgc gtgctggagc tggcggtgga cgagggcgcc gaaatcgccg ccggcgccac
    20821 gctggccgaa ctggatgcga cctccgtcac gctggaagta cgggccgcga aggcggatct
    20881 cgggcgcatc ggcgcgctgc tcgccaacga ggagcgccgc gtcgagcgct ttcgcacgct
    20941 cgcggccaga gagctgatat cgcgcgagca gctcgaggac gccgaggcgc agctcgcggt
    21001 gctgcgcgcg cagcgcgacg ccgtcgaggc gcagctgcgc atcgtcgagg accagctggc
    21061 gaagaccgtg atccgcgcgc cgctcgccgg gcgcatccag aagcgcatgg tctcggtggg
    21121 cgatttcgtg aggcgcggca gcccgctgtt cgagatcgcg accagcgcca gcctgcgcgc
    21181 gctgctgccg gtgccggagc cgctcgcggc caggctggcg cccgggctcg cggtgaagct
    21241 cggctcgccg ctcacgccgg acacgcacgc gaccggcgtg atcaccgagc tgcgccccgc
    21301 ggtgggcgcg gccaaccgcg cggtgtgggc catcgtggac gtggagaacc ccggcgcgtg
    21361 gcgtcccgat gcgacagtgc gcggcgagat cgtgctggcg acgcacgccg acgcggtagt
    21421 ggtgcccggg cagagcgtga tacgtcgccc ggcgggcgag gtggtctacg tgatccgcga
    21481 cggcagggcc gagcagcgcg tggtcggcac gggggagcgc gtcgaagggc tcgtggaaat
    21541 catcgcaggt gtcgccgcgg gcgagacggt ggccgtggag ggtgccgcct acctcagtga
    21601 cgacgcgccg gtacgcctgg cggggaacgc gccgtgaacc tgcccgaaat ctcggtacgc
    21661 cggcacgtgc tggcgtacat gctgtccggc gtgctggtgc tgttcggggt gatcagcttc
    21721 gatcgcatcg gcgtcgaccg caaccccgag gcggacttcc cgatgatcac cgtggtcacg
    21781 cgcctgcccg gcgccaaccc cgagatcatc gactcgagca tcaccaacac catcgagaag
    21841 atcgtcaacg gcgtggccgg catcgaccac gtcgaatcga tatcggcgcc cgaccgctcg
    21901 atcgtgcgcg tgaagttcga gatggacaag gacatcgacg tcgccttcaa cgaggtgcag
    21961 gtcaagatca accagatcct gccgcagttg cccgaggagg ccgacccgcc ggtggtcgcg
    22021 aaggtggaga tcggcggcac cccggtgatg tggctcacgc tcagcggcga ccgcacgctg
    22081 cagcagctga acgtctacgc cgagaacgag gtgaagaaga ggctggagac gatcgcgggc
    22141 gtgggcgagg tgcgcatcgg cggcgagcgc gagcgcacga tccgcgtcga gctcgagctc
    22201 gagcgcatgg ctgccttcgg cgtgacggtg caggacatcc aggccgcgtt ccggcgcgag
    22261 cacgtgcggc tgccgggcgg cttcctggtc ggcggcaccg gggaggacct gctcaaggca
    22321 gacctcgagt ttcacgatct cgagtcgctc gccgggatga tcgtggcgca ccgcgagggt
    22381 gctccgatcc gcctgggcga cgtggcgcgc atcgaggacg gtgtggcgga cttccgcgtg
    22441 ctggcccgct tcaacggcga accgacggta ggcatcggcg tcgtcaaggt caccggggcg
    22501 aacacggtcg agatcgtgaa cgaggtgaag cggcggctcg agtccgagat cgtgccgcag
    22561 ttgcccgcgg gcgtggagat cggcatcgcg cacgacgaag gcgagctgat cgggcagatc
    22621 gtcgcggcgc tggaagagca cctggtgctc ggcacgctgc tgacggcgct cgtggtgctc
    22681 ggcttcctgc gcaacctgcg cgccaccgcg atcgtttcgg cggccatccc ggtctcgctg
    22741 ctgggcgcag tggctgcgat ctacttcctc ggctacacct tcaacaccat gacgctgctc
    22801 ggcctgctgt tgctgatcgg cgtggtggtc gacgacgcga tcgtggtgct cgagagcata
    22861 ttccgccacg gcgagcgcac cggggcggac cggctgagcg cgaccatcga gggcgcgcgc
    22921 gaagtgacga tggcggtggt ggccgcgacg ctcaccctgg tgagcatctt cgcgccggtg
    22981 atcttcatgg aaggcatcgt ggcgcgcatg ttcgaggcct tcgccgtcgt ggtcagcgtc
    23041 ggcgtgctgg cctcgctgct cgtctccctc acgctgacgc ccatgctgtg ctcgcgccac
    23101 ctgcgcctgc attcgggcga gggtgcgatc gcgcggcggc tgggcggcgc cctcgacgcg
    23161 ctggagcgcc actaccggcg catggtcgcg ctcacgctgc gcgcgcgctg gagcgtcgtg
    23221 ctggccacgg tgctcgcggt ggcgtcgagc ctgtggctgt tcggacagat cggcaagggc
    23281 ttcttccccg agatggacga gggctacttc tcggtcaccg tgaaggtgcc gctgggatcc
    23341 tcgatcgagc acgccgtgga ccggctggcg cgcgtcgagg acgtgctgaa gcgccagccc
    23401 gggatcgagg cctatttctc cacgatcggg caggggcagg cggggcaggt cagcgaagcc
    23461 agtatcgtgg tgcacctgcg tcactggtcc gaacgggaag cgagccagca ggaaatcatc
    23521 gaacgcgtcg acgcggagct cgcgaacctg gccggcgtcg aggcattccc gacgccggcc
    23581 tcgatgctcg gcggcatgcg tggcgagccg ctgcacttcg tgctggtcgg tcccgagctc
    23641 gacgaggtcg cgcgtcaggc ctacgcgctg cgcgagcggc tgctgcagga gggcgatctc
    23701 ggcaagctcg acctcgacct gcagctcgcg ctgccgcagc tcgagctcgc ggtggaccgc
    23761 gatcagctgg ccgccgccgg gctcgcgggc ggcgatgtcg cgatggcgct aggcgtgctc
    23821 gcgggcgggt gggaggtggc ggactacaac gacgtgccgg gcgacggcga gcgctacccc
    23881 atccgcctca aggctgccga gggcgaactg gcgcgcgccg aggatctctc gcgcatctgg
    23941 ctgcgcaccg cttcgggcga gatgctgcgc ctggacagcg tcgcgagcct gcgccgcgcc
    24001 gtcggtcccg cggtgatcgc gcgctacgac ctgcaatacg ccgcgaactt ctacaccgcg
    24061 ccggcgatcc ccgagggtgt ggccggtccg cgcgtgctcg agatcgcgcg cgcgatgatg
    24121 ccgaccggct acgaggtgca gctcgcgggc cgcgccgagg aattccagaa gaccgcgagc
    24181 tacatgatgc tgaccttcgt gaccgcgctg atgctggtct acatggtgct ggccagccag
    24241 ttcaactcct ttgtccagcc gctgatcgtg atggtggcgc agccgctggc gatcgtcggc
    24301 ggcgttttcg cgctgtgggc cacggggcac acgatcaacg tgttctcgat gatcggcctg
    24361 gtgctgctgg tggggctggt ggcgaagaac tcgatcctgc tgatcgacct cacgaaccag
    24421 ctgcgcgaac agggccgttc gatcgacgcg gcgctgaccg aggcctgccc gatccgcatg
    24481 cgcccggtcc tcatgacctc gatgacgatc atcctcgcga tgctgccggc ggcgatcggc
    24541 gtgggtgccg gctcggacac caacggaccg ctcgcggtcg cggtcatcgg cggcatggtc
    24601 agctccacgc tgctgacgct ggtggtggtg ccgacggtgt attcgctggt ggagcaccgg
    24661 ctggagcgcc gcatccgccc gtaggtcggc attcatgtgg gtcggcattc atgccgactt
    24721 gtgcctgcgg gaatgcggcg ggttcggcgt tgccgaaccc ttgtcgggtt gaaacccgac
    24781 ctacaagccc gacctacagg ttgtagacca ggtccacgcc ccacatccgc ggcgggatgc
    24841 gccaagccgg tgtcgagttg accgcctcgc cgttgccgaa caccgtcagc acttcttcgt
    24901 cggcgaggtt ggtgccccaa agcgccacct cccagtcctc gtcctggctg aaccagctga
    24961 ggcgcgcgtt ggccaggccg taatcctcga cctcgccgcg cgagggcgcg atgcggtcgt
    25021 cggcccaggt gtagtcgatg cgtcccacca gttcgccgtt ctgcgccagc ggcaagctgt
    25081 actgtgcgct cagcgtgtac ttgtgctcgg gcgagtcggc gcgcggctcg ccggtgaggt
    25141 cgtcggcctc ggtctcgcct gcggccggga tgatgtcgta ggcggtgaac tcggtatcca
    25201 gccaggaata gttgccggcc agcagccagt gctcgccggg catccaggcc gcctcgatct
    25261 cgaagccctt gccctcggca tcggcgttgc gcaggttgta gctcgggatc ggcacgccca
    25321 tcagttccag cgtctgcagg ttctcgtagt cgtagaagaa caccgcgctg ctgtactgca
    25381 gcgtgcccgc gagcgcggtc cccttcacgc cgagctcgta gttcatgacc tcctcggaat
    25441 cgtaggaggt ctcgatgtcg ggaccgaagt tgaggctgtt gaagccgccc gacttgaagc
    25501 cgttggcgat gctcgcgtag gccatcacct catcggtgaa atggtggtcg agcacgatgc
    25561 gtccggacac cgcaccccag ttgtcgctct cggtgctgtc cagcaacggc tgcccgccgt
    25621 tgaagaaggc gagaccgaag ggaatgccgg gctgacccgg gccgggctcg atcaggtagt
    25681 tctggtaggc cgtgtacagg tcgaaggtct tgtcgtcgta ggtgtagcgc aggcccaccg
    25741 tgaggttggt ggtgtcggtg aggctccagg tggtgtcgcc gtagatcgcc cagctcgagt
    25801 agtcgccctg gctctcgacg gtttcgttcc acggctcgtc gtagccggcc gcgatgcgcg
    25861 ggcgcagtgc gtccagaatg tccgagacgc cgagcgtcac gccgttcagg ctggaaatga
    25921 agaacgaggc ggcgatgccg tcgcagttca cgcctgcgca gttcacgccg tacagggcgt
    25981 tgagcagggg gttcaacgca ttcagcgtgg ggttggtgcc ggtgcgcgcg ccggcgcgaa
    26041 aggccggtat ctcctcgggc gtgatcgtca tcccgagctg ttcgaacacc ggtggcagcg
    26101 cgaagctctc gaaggtgctc accaggaact gcgcgtccgt ggtgtgctcg agctcctcgc
    26161 tggtgtaggt cgcgccggcc gtccacttga gccgctcgct ctcgccggtg aagcggaact
    26221 cctgcgagaa gaagtcgctc tgctccggat tcgacgaggc gaagtagaac tcggggttgt
    26281 tggaaccgtc gaggtcctcg agcagttcgg tctcgaagtc gcgccacgcc gttatcgagg
    26341 tgaacgtgac gtcgtcgaac tggtgattga tctcgaggct cgcggaaaac agctcgcgct
    26401 tctcgcgctg ctccggcgcg tcccaggcgc cgtcgccgaa gtcgtcgtgt cgccgtcccg
    26461 ggttcgccgc ctcccacgcg gcgccattcg tggtgtgcag cacgcccgag agctggtcca
    26521 tgtcctcgta gccgatgcgc gccagcacct cggtattcgc ggtcgcgttc cacagcagcg
    26581 cgaggcgcac gttcttcttg tcctcctcgt tgacgtcgtc gccgccggtg aggtccttga
    26641 tgtagccgtc gcggcgctgc acggccgcgg tgaggcgcat cgccacgtct tcgccgagcg
    26701 cggtgttgta gatgccctcg atgtcgcggc ggtcgtaatt gccgccggtc accttgatgc
    26761 ggccctcggt cgcgaacgcg ggcctgttgg tgatgatgtg gatggcgccg cccgtggcgt
    26821 tgcgcccgaa cagcgtgccc tgcgggccct tgagcacctc gacgcgctcg acgtcgatga
    26881 aggcggcctc ggcgccggcg ccgcgcgcgg cgtacacgcc atcgacgtag accgccacgg
    26941 ccgggtcgga gccgatggtc cagtcgttgg tctgcacgcc gcggatgctg taggtgggtt
    27001 gcagcgactt gtcgttgttc atgtccacgc cggccgtgaa gcgaccgatg tcggtgaggt
    27061 tgcgcgtgcc gagctgttcc agcgcctcgc ccgaataggc ggtgatcgcg atcggcacgt
    27121 ccatcacgtt ctcggcgcgc ttctgcgccg tgacgatgat ctcctcgatc acctgcgcgt
    27181 gcagtggctg gctggcggct atggcgaagg cgatggcggc gcggcgaaag gtcatggcgt
    27241 gctccgtcgg tagcgttgtc gttgtccgga ccgggcgcac catcccgggg cgccaatcgg
    27301 ccggttaagc ttaggcgttg tgcgcccggg atgcgaatgg gcgaannnnn nnnnnnnnnn
    27361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnngaatg ggcgaaaaag acgcgcgaac gcgcgaaaac
    27481 ccgcgatctt gacccggatc gtgcctcgcg ctggcgccag gcgcaccaga acgggtagca
    27541 ttcgccctct tttttgcact ggaacggagc gccgcggcgc ctgcaggaga aaccggcacc
    27601 atgaccaccc cactcgacga gaagctcgaa cccatctacc aggaagtgct gcgccgcaac
    27661 cccggcgagc tcgaattcca ccaggccgtg cgcgaggtac tggagtgcct cgggccggtg
    27721 atggtcaagc acccggagtt cgtcagcaag aagatcatcg agcgcatctg cgagcccgag
    27781 cgccagatca tcttccgcgt cccctggcag gacgacaggg gcgaggtgca gatcaaccgc
    27841 ggctttcgcg tggagttcaa cagtgcgctc gggccctaca agggcgggct gcgcttccac
    27901 ccctcggtgt acctcgggat catcaagttc ctcggcttcg agcagatctt caagaacagc
    27961 ctcaccgggc tgccgatcgg cggcggcaag ggcggctccg acttcgaccc caagggcaag
    28021 tccgacggcg agatcatgcg tttttgccag agcttcatga ccgagctgta ccgccacctc
    28081 ggcgagtaca ccgacgtgcc tgcgggcgat atcggcgtgg gcgggcgcga gctcggcttc
    28141 atgttcggcc agtacaagcg catcaccaac cgctacgagt cgggcgtgct cacgggcaag
    28201 ggcctgaact ggggcggttc gctggtgcgc accgaggcca ccggctacgg cgcgaccttc
    28261 ttcgtggccg agatgctgcg cgcgcgcaag gattcgctgg agggcaagac ctgcgtggtc
    28321 tcgggctcgg gcaacgtggc gatctatacg atcgagaaga tccacgagct cggcggcaag
    28381 gtcgtggcct gctcggactc caacggctac atctacgccg aggacggcat caagctcgag
    28441 ctggtgcagc agatcaagga agtcgagcgc agccgcatca gcgtctatgc cgagcgcgag
    28501 aagaaggcgc gctacgtggc cggcggcaac ctctgggaca ttccctgcca ggtcgcgatg
    28561 ccctcggcga cgcagaacga gctcaacggg cgcgacgcgc gcaagctggt gcagaacggc
    28621 tgcatcgcgg tgggcgaggg cgccaacatg ccgaccacgc ccgacggtgt gcgcgtgttc
    28681 ctggacgcga agatcgccta cggcccgggc aaggccgcca acgccggcgg cgtggccacc
    28741 agcgcgctgg agatgcagca gaacgcgagc cgcgattcct ggagcttcga gcacaccgag
    28801 aagcgcctgc accagatcat gcgcaacatc cacgacctgt gctacgagac cgccgaggaa
    28861 tatggcacgc cgggcaacta cgtgaacggc gccaatatcg ccggcttcat ccgggtggcc
    28921 agcgccatgg tggcgatggg cctgatctga acggcggcga cgggaggttc acgacatggg
    28981 caggctgact ggcaaggttg cactgatcac cggcggtgcg cgcggcatgg gtgccgccgc
    29041 tgtgcgcatc ttcgtgcgcg agggcgcgcg cgtgctgatc gcggacgtgc tcgaggagcc
    29101 ggggcgggcg ctcgcggcgc agctgggcga cgccgcgcgc ttccagcgcc tggacgtgag
    29161 ccaggaaggc gagtgggagg ccgcgatggc cgcggccgag gccatgggtc cgctgaacgt
    29221 gctggtgaac aacgcggcga tcctgcatgc cgcggcgatc acgcagacca gcctcgccga
    29281 ctaccagcgc gtgatcaacg tgaaccaggt cggtaccttc ctcggcatcc gcgcggcgat
    29341 cgagcccatg aagcgcgccg gcggtggttc gatcgtgaac atctcctcca tcgacgggtt
    29401 gcagtcgaag aacgggctca tcgcctacag tgcctcgaag tgggcggtac gcgggatgac
    29461 gaagtgtgcc gccatcgaac tggggcgtta cggtatccgc gtcaactcgg tgcatcccgg
    29521 cggcatcgat accgtcatgg gcaacccggg cgcgatgtcc ttcgagaagg tcagcgagtt
    29581 ctacgagcac cacgcgctgc cgcgcgtcgg cctgcccgag gaaatcgcca acatggcgct
    29641 gttcctggcc tccgacgagg cgagctactg caccggctcg gagtatcccg tagacggcgg
    29701 ctggaacgcg gggttgcgca acgagcggtt gccggg
//
LOCUS       DAORUQ010000017        29571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1120_length_29571_cov_6.061052, whole genome shotgun sequence.
ACCESSION   DAORUQ010000017 DAORUQ010000000
VERSION     DAORUQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29571
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_29571_cov_6.061052"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLW13_02990"
     CDS             <1..813
                     /locus_tag="PLW13_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HQY69363.1"
                     /translation="YLRLEVRRDNEPAIRLYRKLGYRLFGTIPHYYEDDQEALRFEKR
                     IRFYSGARSDLFVPYYGQTTPFTCGPACLLMAMRAIDPQAPVDQREELRLWREATTIF
                     MTSGHGGCGPHGLALAAQRRGFNAELFLNDDRVLFIDGLRDERKRQVLGVVHEDYHQQ
                     VLAAGIPISYQPLTVEQMATCLARRIVPVVLISSWRLTREKAPHWVVVAAIDSDFVYI
                     HDPDIKTERGESDTDKAHVPIDRAAFTRMARFGQSSTRATVLLSPGRKRRGA"
     gene            836..2533
                     /locus_tag="PLW13_02995"
     CDS             836..2533
                     /locus_tag="PLW13_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY69364.1"
                     /translation="MHPDFLLQTLVLLGAIVIVVPLLTRLGLGSVPGYLVAGLIVGPP
                     LLGWVAEYELIGKVAEFGVVFLLFSIGIELRPARLWVMRRLVFGLGSGQLLLCAVAIA
                     VLLSWGGMALPAATVTALALALSSTAIVVQLLSERGELSSQRGRVTFAVLLLQDVAVA
                     PLLMLVYLLGRGGTEAAFSPAGVLEGLAMLAAVLLLGRLVWRRVLPFLAAQRNADTLV
                     AVALLLVLSAAWLMEHAGMSLAMGAFLAGVLLADSPFRHQVVADIQPFRGILLGLFFM
                     TVGMSIERQAVLEHGLMLLAIALALMAVKTLVTFGVMRTFRIPAGEALHGALLLSQTG
                     EFALILFALAAGENLISAAERQMLYQVVVFSMLLTPLADLLGRRLAGAMPRTAQASHA
                     PNAADHEAAHGQVIICGFGRVGHQVARLLDAAGQRWIATDLDSEAIEQARQRGQQVFY
                     GDCSRAEILRALGVDRAALVLITLNDAGATERALIAIRQLVPQARVIARAHDPAQAHT
                     LRAHGVLRAVPETIEYSLQFGLAALSELGVDAAYRQELEDALRADEYTRLLSDPPSQ"
     gene            complement(2641..3123)
                     /locus_tag="PLW13_03000"
     CDS             complement(2641..3123)
                     /locus_tag="PLW13_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY69365.1"
                     /translation="MQIASGTVASFHYTLRAEDGAEIENSRNGEPVVYLHGHGNILPA
                     LEQQLAGHAAGDTLSVTLEPEQAYGVRRADSIQRVPLKHLHAQGRLRPGMVVAVETEH
                     GARQVTVLKVGKFNADVDTNHPLAGRTLGFEIEVLEVRAASEEEIAHGHAHGAGGHHH
                     "
     gene            complement(3204..4250)
                     /gene="rlmM"
                     /locus_tag="PLW13_03005"
     CDS             complement(3204..4250)
                     /gene="rlmM"
                     /locus_tag="PLW13_03005"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: protein motif:HMM:NF008734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HQY69366.1"
                     /translation="MPDTLVLLCRPGFEKDCSAEIIDSLARTGVAAWCRAEAGTGLVE
                     VRSGGDWDARSALAGIRFDELVFARDWLAGRVLEDLPVGDRIAPLLAAARALGPFADC
                     WLQHVDTEEGRSVGKLCTRLQARLEGALGSEQLLARRATMRLHVLFLSGSKAFVGVSP
                     VANGARWSMGIPRLRLPGAPSRSAAKLEEALLYFLGDEAERLLRSGMSAVDLGAAPGG
                     WTWILARRGLAVTAVDRGALSPQAQHAGAVRHLAQDAFPFRPAHPVDWLVCDIVDKPA
                     RVAELIERWFLNGWCRQAIFNLKLPMKQRYRDAALMLARIVAELEDGGGRVACTARQL
                     YHDREEITVWLRRI"
     gene            complement(4354..5031)
                     /locus_tag="PLW13_03010"
     CDS             complement(4354..5031)
                     /locus_tag="PLW13_03010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQY69367.1"
                     /translation="MRSIRLVVVLALGVACFAATPREGCADAFSPDAYYRLRMDLFAA
                     TPLGAGDIVFLGDSITEGAEWSELFPDLPVRDRGISGDTTAGVLARLDTVTASRPAAV
                     FLLIGTNELAPSLDPAPSLARQREILERIRRESPDTRVFVQSLLPRAGTYRERVESYN
                     DALRGICREAGVGFIDLYPAFLAPDGSLRDELTFDELHLSGAGYRHWRELLRPYLQAV
                     GPASAGH"
     gene            complement(5255..5461)
                     /locus_tag="PLW13_03015"
     CDS             complement(5255..5461)
                     /locus_tag="PLW13_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69368.1"
                     /translation="MAKPSRSSTQANAAVPTAEHQRIIAEKTAAFLAGGGEIQQIPKG
                     VSGYGKLGGPQVPVAPAARAARTT"
     gene            5794..6210
                     /locus_tag="PLW13_03020"
     CDS             5794..6210
                     /locus_tag="PLW13_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY69369.1"
                     /translation="MTRPGRIKPPMGHDNAWWWEQAAKGVLAIQRCSRCQALRHPPRP
                     MCGECRSMEWDVLAASGKGTVASYTVLYHPQFPGFEYPLIIVLVDLEEGTRITAELKD
                     CDPEAVDFGMAVTAYIHEDEDGFRIPMFRPATRGGN"
     gene            6212..7381
                     /locus_tag="PLW13_03025"
     CDS             6212..7381
                     /locus_tag="PLW13_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HQY69370.1"
                     /translation="MPISMKNEAAIAGIGATKFSRNSGVSELALACEAVSNCLDDAGL
                     APGEVDGLVTYTLESNDEVEVARAVGLGDLTMYAKINYGGGAAVGLIQQAAMAVATGA
                     AKNVVVWRAMNGRSGQRMGQGVSGNIISSDLIHWSWYMAYGMLTPASWVAMIANTYMH
                     RFGVAAEHLAEVAIAQRNFALNNPAAAGYGKPLTLQEYMGSKRITERLRIYDCCQETD
                     GGVALLITSTERARDLRHKPAVIRGVTQASTRGQEQMTSFYREELDRLPEMELAARRV
                     YAMSGLGPEDIDAACLYDAFTPEVIMQLESFGFCKRGEAKDFVAAGNLRLGGRLPNNT
                     HGGLLSESYIHGINNIAEGARLIRGTSCNQPERVEHVLVSSGVGVPTGALILGRD"
     gene            complement(7423..8328)
                     /locus_tag="PLW13_03030"
     CDS             complement(7423..8328)
                     /locus_tag="PLW13_03030"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQY69371.1"
                     /translation="MLNKVVVVTGANAGIGFQAALNLSRMGAQVVLACRSMDRARKAH
                     GEILAEVPDARAVIIPLDVSEPASIQEFARQFSGQMGQLDLLINNAGIVATPLARNSV
                     GQEMQLATNYLGAFALTGTLLPFFTRTTQARIVNVGSLMHRFGKLDLDDLNWERTPYN
                     EWQGYGRSKVAMLSFTMELDRRLRKSDSNIIALGAHPGFANTDVAKNSAGLKAQSGLR
                     AWRQKKLEPLIPTAAEAVRPIIHAATAQGVSGGDYYGPSGFLEIGGKTTKKARVNPVA
                     KKADVGKRLWTLSETMTGIEYLSDL"
     gene            complement(8375..8740)
                     /locus_tag="PLW13_03035"
     CDS             complement(8375..8740)
                     /locus_tag="PLW13_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQY69372.1"
                     /translation="MKVKAARVDDIAEGRSKCVKLDGLRILLVREKGEIFALENRCPH
                     FGLSMEKGAISNGNITCPWHGSSFDVKTGANVDWVNSVAGIALPRWSQKLVAMGKEPQ
                     AVRTFPVTVENGTVFVDTA"
     gene            complement(8811..9293)
                     /locus_tag="PLW13_03040"
     CDS             complement(8811..9293)
                     /locus_tag="PLW13_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69373.1"
                     /translation="MTKQKGLMGRIGEAVDAARRAVNKGARAVQKTAHDAEIAVERKA
                     KSVKADVKSATGKAERQVKRKVKTAAARVKKAAVKVEKAVSPKKPAARKAATPGMKLD
                     KGIASVKRATKKAEAAVVEAVKAASATAGKTPARKAPAKKAPAKKAPAKKAPAKRTAK
                     "
     gene            complement(9468..10916)
                     /locus_tag="PLW13_03045"
     CDS             complement(9468..10916)
                     /locus_tag="PLW13_03045"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HQY69374.1"
                     /translation="MSLPPEVPGEAFVPGTRLLGNLPGVPYVEEEFFVEGHATLFNYA
                     HNPPLGPTDLAAIDADVPYRTRLIVRRPVDPKAFNGTVVIEWWNSTAGFDGAPVWDQS
                     AEYFARRGIAYVGVSNSTTAIAFLAGGCRVFGVLPPSCGSRYATLSLPENGLAYEMMS
                     QIANLLKSDDPHNPLPQAYRVRRLFHAGESQQAGSVITYASAFHLDDVNDGYFIQSNV
                     HARPINFGPRCDDAGAPAFPECTPRLAHPDSLVRTDLPVPVYQVITQTDFETLNFNVL
                     GRQPDTPSFRYFEVAGAAHNVVHKDIELVPAGLLGPDALGLADLCASAMNSTADGPVF
                     ASYVLNALWARMETHADFRGYYRIVDRRVPAGQVMEAIDGVLARDALGNVIGGVRLPA
                     LEAPTATYLSTNTASPDLPPALLAIGNLACRLSGSVLPFDQATLDALYPNPASYLVRV
                     RQSVDALQRQGLLLPEDAAKVMSDAVRSLTAR"
     gene            complement(11064..12272)
                     /locus_tag="PLW13_03050"
     CDS             complement(11064..12272)
                     /locus_tag="PLW13_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY69375.1"
                     /translation="MSAAELLPWGDPAFWQNPHPVLAGFRAKHRTAFTDTGQLAVLRW
                     DDAEWAIRGHDFINEGIERLEARGFRPGDPLHTWRSHALGIMEGPDHTRIRKLVTGAM
                     SRRNMEPLRPLIRSIAGQLIDRFPRTGSVDIQRGFTAQLPRLVMMEFLGIGADELMGS
                     MGPLADARIADCFGQNVTQAMRDKANGAIRMVMDHVASLYEKRRREPRDDLLTSLLEA
                     HDDRGALSMPELITLFSTIFGSGSTTAGTLDAGLLELALHPEQQALFRSDPGKWKRGV
                     CEETLRMHPGIAEMPQKAAHDFEAFGHSFRTNDTIMIPLHSPNRDPQRWQDPEEFRIS
                     RDPDVWHLSFGIGAHFCLGQAMARYTIEEALAVFVERCRSFELAQRPEWEPCVMENRL
                     RSLVLNVDMD"
     gene            complement(12335..13903)
                     /locus_tag="PLW13_03055"
     CDS             complement(12335..13903)
                     /locus_tag="PLW13_03055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY69376.1"
                     /translation="MRSLKLCLCIFSVGVLSACGVNPVTGDKEIQFVSEAQEVKMGEQ
                     YYAPTRQSEGGDFTVMPELTDYVDEVGQKLAAVADRKLPYEFVVLNSSVPNAWALPGG
                     KIAVNRGLLTELQNEAELAAVLGHEIVHAAARHGAKAQERGTLLQVGMAAAQIGAAIG
                     GVDQNTANLAIQGAGVGAQMIQQKYSREQELDGDQYGMKYMKAAGYDPWGAVTLQETF
                     VRLSESGAKKQGWLEGLFASHPPSAERVAQNRLAAEQLGRGGELGAERYAARMKPLRA
                     IKPAYDKYDKAIVAARRKDFAAARSLTGEAIQLLPREGRFHELMGELALAEKRPEAAI
                     PHYQKATALNPGYFGSWLGGGVAQLQAGNKPKAEEWLNRSVELLPTAPAAYYLGNIAR
                     DRGDGARAMSYYRAAAKSSGPIGQAAAAEFMRMDLPQNPGNYVTAKGQFDAEGRLYAV
                     VRNRAPVALRDVQLTPVLVNNAGRVVQQGSPLRLRGTLAPGAQTAVPAGVGGIAREQL
                     PYVRFHVDGARVAE"
     gene            14090..14959
                     /locus_tag="PLW13_03060"
     CDS             14090..14959
                     /locus_tag="PLW13_03060"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HQY69377.1"
                     /translation="MATPTGMKLARLPRIALVVLFALALSACGTRLLYERIDTLLYFQ
                     LASQVTLDDGQARALRASLRELHAWHRGAALPAYAEFLETLADDMAQPVGAGGIDAAR
                     IALEGLWRDTVAGVAPTAARWLAGLDPAQREEFFAGLAERDVELRREYCDIGDEERRR
                     NRERAVVSALGDWVGRLGREQRTLVREQLRGIDANGCDWIASRAGFRERLRQLVDTHA
                     AQPHYEARVARLLMHPEDGWDAAYRARYAANSAVVVTMLAELDRTLSERQRARLGAKL
                     RRYALDFRELAAI"
     gene            complement(14990..15925)
                     /locus_tag="PLW13_03065"
     CDS             complement(14990..15925)
                     /locus_tag="PLW13_03065"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69378.1"
                     /translation="MSRLPAIVCLLLVCFAARGGTFPGAPAEVEAYLAARERLEPGIT
                     PGAERAVRWAGGEPARTPLALVYLHGYSATRQEVFPLAERVAAALGANLFHTRLTGHG
                     RDGAAMLEGSVAAWQQDTLEALAIGRAIGERVVLLSTSTGGTLSTWAASRVHDDSLAA
                     LVMISPNFAPRDRTLYLLDWPLLGPALLGYLGEGYRSWQPHNARQARYWTWSYPYRAL
                     PELVRLVKEVAALDKSAIGVPTLMIYSPADRVIDPAAVAEAFAEWGGERKRLVVFERS
                     ADPSQHVLAGDILSPGSTEELARMITDYLAGLAGS"
     gene            16200..16862
                     /locus_tag="PLW13_03070"
     CDS             16200..16862
                     /locus_tag="PLW13_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY69379.1"
                     /translation="MKILLAEDDKRLSERLRTDLKRRGYAVDVADNGIDAEHLGTEWP
                     YDAAILDLGLPQRPGLEVLRNWRRQGIALPVLILTARDAWHEKVEGFNAGADDYVAKP
                     FHVEELLARLGALIRRAHAQPAGVVSAEGLTLDETRQVALRGEREIALSGIEFRLLRY
                     FMLHPGQVLSKSHLAEHLYASDADPESNVIEVHVNRLRGKLGADCITTRRGQGYVFGA
                     PA"
     gene            16862..18244
                     /locus_tag="PLW13_03075"
     CDS             16862..18244
                     /locus_tag="PLW13_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQY69380.1"
                     /translation="MRSLLAPLVAGLVLSLAAVFGVQWSMFRIEFDAMIVEYIAHELT
                     HDAEEIFGSLTVLPSGETRLALTHFDPPFLRPGSGRYFQILVDDGTVLRSPSLDGESL
                     AVPTVNRGRRRVTHVAGPHEQDLLISAVGYEFAGRQLTIGVAADLNPVRSQFERLMTH
                     YSRVSVLMFALLVALQVAIVRLAFGTLRRVKADVGRLERGEITQLGERVPAEVLPLVQ
                     EINRLLALLTRRLQRSREALGNLAHALKTPLTVLQHMADDDQFQRDPELARQMAEQVS
                     ILRSRIDRELRRARVAGGRSTGAPVAVAKEIDALAQTMGKLHRERNLDIACRIDPEAA
                     FSGDREDFMEMCGNLLDNACKWARSRVRVTVHDGGALALSVEDDGPGCSPGDLERIAR
                     RGVRLDEATEGHGLGLAIVDSIASSYGAELHFGRSPELGGFAVTVSFPGTQRPVRGVN
                     RTATPDNANS"
     gene            18241..19485
                     /locus_tag="PLW13_03080"
     CDS             18241..19485
                     /locus_tag="PLW13_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69381.1"
                     /translation="MTELPTLSAMLAAITRLEPLLRAQMPAGRRAARLPAPVVEQLLR
                     LGLFRLWIPRRYGGLEMSLPAALAIHEAVAQLDGSTGWAVMIGSGGGLFAAWLAPPVA
                     GRLFGAADALVAGSGAPNGSAARVPGGFRVTGRWRYASGADHASVFTANCRVTEPGSN
                     PAPLIRAMAFAPADVKIHRTWDSSGLRATGSHDIEVRDVFVPEEMSFSVFTDPVLENG
                     PLYRLAFATLTELPVSAVALGIARRACEEFAALAGSKCAPGSTVALLGLDLVQAALHR
                     ARDVVANAAREQHAQAAEVWEEALAGIRPAPHVGAARTVACVRRVRGLVTAVGDLVPL
                     AGMNALQREDPFALAWQDLNAAAAHYSVSPLQLAEQDGRDIAARGRSYDLPVASSPLA
                     SCDAPSPTGNGAAAARPPDSGE"
     gene            complement(19383..20624)
                     /locus_tag="PLW13_03085"
     CDS             complement(19383..20624)
                     /locus_tag="PLW13_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY69382.1"
                     /translation="MIDEAAPPPAPAPAPAGATPSARAPAGAEQGRIVRWFFFGAFAF
                     LIYQLLLILSLFSDAIIWAASLALVCFPIHKFLQRRLPSREGVAAGLSTLAVLLLVLV
                     PTLFVLGVVVRQSAELYPTVRGWIGAFQSPEGAFSASLLPGFLQGYWHRLSEFAGQIS
                     LFAQFDLQEFMLGNINVASAKIANVGAVMARNILLGVVNLMLILVLLFFCFRDGERFL
                     HWLFDTVPMPVAQAQSIALRIYQTVTAVIRGALLTAVIQGLLAMIGYQIAGVPLAVFF
                     GVMTGFAAMIPVVGAGLVWAPIGVFIFMKSPAWGVFVFAWGFFLVSLADNVLKPILIG
                     SRARMPILLIFCGIIGGASVYGVTGLIIGPIVIASLLAFVTIYREHYSPESGGLAAAA
                     PFPVGEGASQDASGDDATGRS"
     gene            complement(20656..23814)
                     /locus_tag="PLW13_03090"
     CDS             complement(20656..23814)
                     /locus_tag="PLW13_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY69383.1"
                     /translation="MQRLIAWWVDNPVAANLLMLLFIAGGLISLSQLRKEEFPNLELD
                     YIQITVPYLGAAPEEVEAGVCLRIEEAIEGTEGIRKIMTSAAEGLCSVLVQLHHDADK
                     SKALDDVNARVYAINTFPVEAERPVINEVTVMTTVMELAISGHTDERALKELAEEVRE
                     ELTEDPRISQVRLQYARPYEIAVEVSEHTLRQYGLTFDRIAQAIRDASLDVPGGLVKS
                     AGGEILLRTKGQRYTGGEFRDIVVTKRADGTALTLGEIATLVDGFEDTDLRVRYDGKP
                     ALLLQVSRVGEEDTLEIAGAVKDYLGRKRAQLPEGMAIDISRDESQDLVDRLDALLGN
                     AWSGLVLVVVVLALFLRLRIALWVSVGMPVAMLGALAMFPAAGLTISSLSLMGFLLVL
                     GIIVDDATVVGERVHAFELEGMAPREAAIRGASEVSVPVIFGVLTTQAAFLPIITLEG
                     GMASFFAAVGLTVVLCLCFSLLESQLVLPAHLARQTVRRAGARDENWLERLQDRISAR
                     LERFIADSYQPLMRRAIAHRYVVCAIGVAVMIVMVGLLASGRIVFQYFPSVEGDRLFA
                     SLTMPEGTPAEVTQQAARRLEASAEALRERLDGGRRDDAEPSAVRHVMASVGKRVGRG
                     SLGSDAGEGSHVAEVLVELRPYAERGGMGSAEAAALWRQLTGPIPDALELTFTAEAFS
                     AGKPIDIELRGRDVHQLGEAASELRETLARYDGVFDVSDTYRAGKQEILLAIRPEAEL
                     LGLSQRDLGRQVRQAFYGEEAQRVQRGRDDVRVMVRLPQAERVSLGDLENMRIRTAQG
                     VEVPSMSVAETTVGRGHSTIRRVDGQRVVSVQGDVDREVVVPEKVLASVADTEFPRLE
                     AKFPGISFALAGEAEERAEAMGSLVGMTLLALLIIYALLAVPLRSYLQPFVIMSAIPF
                     GVIGALLGHLIMGQALAFFSLLGIVALSGVVVNASLVLVDYVNQQRLAGVAVEESVLR
                     AGAVRFRPILLTSATTFIGLAPLMATADISTMMFVPLAISLAFGVLFATVITLLLVPA
                     LYLILEDFLGWARAMPGRGRLQ"
     gene            complement(23819..25027)
                     /locus_tag="PLW13_03095"
     CDS             complement(23819..25027)
                     /locus_tag="PLW13_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY69384.1"
                     /translation="MTAMEPQTPIFAAGAGRRRQLLLAAAVIAAGCLVAIVLYATRPV
                     LQPQQLERPLLAVRTVQAVPVDVPLIVRSQGTVEPHTEAELIPEVSGRVVWMSPSLRS
                     GGRFAAAELLLRIDDSDYRSGVGAAEAVLMRAQAEAEFARFESERMATLLARELASRS
                     QAENASRARRVAESVLKEAEVALERARLDLARTAISAPFGGRVRSARVDIGQTLSRGD
                     RIATLYATDYMEVRLPIADRQLAFLDPALIRSGIAPGGDAAPVSLHAEFGGQRWNWEG
                     RIVRTEGEIDAGSRMVHVVARVENPDAPEQAPLPVGLFVQAEIRGQTARGVIELPRAA
                     LRDGDRVLVVDAEDRLRFRPVTLLRADRDTVLVSSGIEAGERVCVSPLQLVVDGMRVA
                     PVASDTDAES"
     gene            complement(25024..28377)
                     /locus_tag="PLW13_03100"
     CDS             complement(25024..28377)
                     /locus_tag="PLW13_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY69385.1"
                     /translation="MPGASAASVASADAAVATPTLEAMERQLEVYRAGSAEDPQNARL
                     IEIYLENVQNLHAVAENRENQARYERLFQESPAKVARFTAGLNDLQRQARSAKGLGQG
                     VAVERLERLLRETRSRLAELRARETSLNNSANSLQGRVESSRQELKATLSATEPPAKA
                     PARVAGESEAMSQARMDQWLVDRELKASRIARLETEARTIPERLHAVQTELKLVTAQR
                     EQTEQFLAELLGMDNLKRIGEAEQLREDVRAQLAGPGSAHPAIVALREEIAALSDEYV
                     KVVGRSESAGADVDRATAQLAEVSSAYDSIRQQLEIAALSDALGPVLMEQYAKLGSYG
                     QPALDLGTTAELLSGTRLREFQVARLLSAEVQLRETVYRALEADTGLSAAELAAAVAE
                     ADRLLSARNRLLEALNRTYTVFTGQIVDLEQAYREQSEVAAKFRELVDRNLIWMKSHP
                     KLGLADFIAWPRATAALLAAQDWSGLGSQLRTHALAHPASALLALLVLVLVSWYRKPL
                     ADRIAAISLRRVGWRDYRYRMGLMAFGIHLLVALPVPLLLLWASWLLARMEGDSALAR
                     ALSDASYRTAGLSYLYLVILQAMGTDGFARGHLRWKTARVLSVRRLLRAGLWVMLPLA
                     FVSTTVALLAGATAASQAYRVTGLMVTVAFFVFLVLILRAGRDMFDSAFYSRGHPMLS
                     RIGWILLVAIVVAQPLVVLLDVQGYHFTARELQLRVFLSSVVLVAAKMLLDAGLLGLT
                     IAARRSLAAAQDTVAQEEPANVAGTAPPPRAAGDAFEEIDLEKMNASAVALLQVAVAG
                     AAALLMVLIWQQFFTALSIFDSVALWSYADTVDGKETVSTVSAFDAGGALLVMALGMV
                     LASGIPSLIGIVFYSVITEKGVLYAIQTVIRYVIAVLAAFFALQMLGFGWSKLQWMAA
                     GLSVGLGFGLQAIFANFFSGLIMLFERPVRIGDVITLGEFSGTINRIRMRATTITDFD
                     NREIIVPNQMFVTERLINWTLSSSVVRFSFDVGVSYDADPRVVRETLLEILHADPRVM
                     KEPAPNVIFREFGPSSLSMRCFAHVEDLALRFQVQNDLHMRIAEVFRDKGIEIAYPQM
                     DLHVRSVDGGWKPSPAAPGAGAAANGETE"
     gene            28609..29178
                     /locus_tag="PLW13_03105"
     CDS             28609..29178
                     /locus_tag="PLW13_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004144723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQY69386.1"
                     /translation="MKRRIIVGISGASGVVYGVRLLQQLRALGDVETHLVVSEGGART
                     LAVETDWRPDDLRGLCDVWHRHDNLAASIASGSFLTHGMIVAPCSIRTLSGIANCYAE
                     NLMLRAADVTLKERRRLVLMVRETPLHKGHLELMLRATDYGAQILPPSPAFYHGRQTL
                     DEVIDQTVGRALDCLGLQHELVPRWGPPA"
     gene            29344..>29571
                     /locus_tag="PLW13_03110"
     CDS             29344..>29571
                     /locus_tag="PLW13_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQY69387.1"
                     /translation="MQLKGSKTAKNLADAFAGESQANRRYLYFAAKADVEGAPDVAAV
                     FRSTAEGETGHAHGHLEYLEQVGDPATGLPIG"
BASE COUNT         4644 a        10455 c         9935 g         4537 t
ORIGIN      
        1 tacctgcgcc tcgaggtgcg ccgcgacaac gagcccgcga tccggctcta ccgcaagctg
       61 ggctaccgcc tgttcggcac catcccgcac tactacgagg atgaccagga ggcgctgcgc
      121 ttcgagaaac gcatccgctt ctacagcggc gcgcgctcgg acctgttcgt cccctactac
      181 ggccagacca cgccgttcac ctgcggcccg gcctgcctgc tgatggcgat gcgcgcgatc
      241 gacccgcagg cgcctgtcga ccagcgcgag gaattgcggc tgtggcgcga ggcgaccacg
      301 atcttcatga cctccgggca cggcggctgc gggccgcacg ggctggcgct ggcggcgcag
      361 cggcgcggct tcaatgccga gctgttcctg aacgacgacc gcgtgctgtt catcgacggc
      421 ctgcgcgacg agcgcaagcg ccaggtgctc ggagtggtcc atgaggacta ccaccagcag
      481 gtgctcgcag ccggcatccc gatcagctac cagccgctca cggtcgagca gatggcgacc
      541 tgcctcgcca ggcgcatcgt gccggtcgta ttgatcagct cctggcgcct cacgcgcgag
      601 aaggcgcccc actgggtggt ggtcgcggcg atcgacagcg acttcgtcta catccacgac
      661 cccgacatca agaccgagcg cggcgagagc gacaccgaca aggcccacgt cccgatcgac
      721 cgtgccgcgt tcacgcgcat ggcacgcttc ggccagtcct cgacccgcgc cacggtgctg
      781 ctgagccccg ggcgcaagcg gcgcggcgca tgaggccgcg gcgaagaacc cgctgatgca
      841 ccccgacttc ctgctgcaga cgctggtcct gctcggcgcc atcgtgatcg tggtgccgct
      901 gctcacccgg ctcgggctcg gctccgtgcc cggttacctg gtcgcgggcc tgatcgtggg
      961 gccgccgctg ctcggctggg tcgccgagta cgagctgatc ggcaaggtgg cggaattcgg
     1021 cgtggtgttc ctgctgttct cgatcggcat cgagctgcgc ccggcccgcc tctgggtgat
     1081 gcgtcgcctg gtcttcgggc tcggcagcgg ccagctgctg ctgtgcgccg tggccatcgc
     1141 cgtcctgctg tcgtggggcg gcatggcgct gccggccgcg accgtgaccg cgctcgcgct
     1201 ggcgctctcc tcgaccgcga tagtggtgca gctgctctcc gagcgcggcg agctctccag
     1261 ccagcgcggg cgcgtcacct tcgcggtgtt gttgctgcag gacgtggccg tggccccgct
     1321 gctcatgctg gtctacctgc tgggcagggg tggcaccgag gcggcgtttt cacccgccgg
     1381 cgtgctcgag gggctcgcaa tgcttgccgc cgtgctgctg ctcggtcgcc tggtgtggcg
     1441 gcgcgtgctg ccgttcctcg ccgcgcagcg caacgccgac accctggtcg cagtggcgct
     1501 gctgctggtg ctgagcgcgg cgtggctgat ggagcacgcc ggcatgtcgc tcgcgatggg
     1561 ggcctttctc gccggggtgc tgctcgcgga ctcgccgttc cgtcaccagg tggtcgccga
     1621 catccagccc tttcgcggca tcctgctcgg gctgttcttc atgaccgtgg gcatgtccat
     1681 cgagcgccag gccgtgctcg agcacgggct gatgctgctc gcgatcgcgc tggcgctgat
     1741 ggccgtcaag accctggtca ccttcggcgt catgaggacg ttcaggatcc ctgccggcga
     1801 ggcgctgcat ggcgcgctgc tgctgtcgca gaccggggaa ttcgccctga tcctgttcgc
     1861 gttggccgcg ggcgagaacc tcatcagcgc cgccgagcgc cagatgctct accaggtggt
     1921 ggttttcagc atgctgctca caccgctggc ggacctgctc gggcggcgcc tcgcgggagc
     1981 aatgccgcgc acggcccagg ccagccacgc cccgaacgcc gccgatcacg aggccgcgca
     2041 cggccaggtg atcatctgcg gcttcgggcg cgtgggccac caggtcgcgc ggctgctgga
     2101 tgcggcgggc cagcgctgga ttgctaccga tctcgacagc gaggcgatcg agcaggcgcg
     2161 ccagcgcgga cagcaggtgt tctacggcga ttgcagccgc gcggaaatcc tgcgcgcgct
     2221 cggcgtggac cgtgccgcgc tggtgctgat cacgctgaac gacgccggcg cgaccgagcg
     2281 cgccctgatc gcaatccgcc agctggtgcc gcaggcgcgc gtgatcgcgc gcgcccacga
     2341 ccccgcgcag gcgcacacgc tgcgcgcgca cggcgtgctg cgcgcggtgc cggagaccat
     2401 cgagtacagc ctgcaattcg gcctcgcggc cttgagcgaa ctgggcgtgg atgccgcgta
     2461 ccggcaggaa ctggaggacg cgctgcgtgc cgacgaatac acccgcctgc tgagcgaccc
     2521 gccctcgcag tgaggagcac ctcgccgtgc agagcctcga tctgcgcggc cgcgaagccg
     2581 gcatgccggc gccccgggag gccgcgcggc aaaccgcggg cctgcgttac gagcggacaa
     2641 tcaatggtga tgcccgcccg cgccgtgcgc gtggccgtgg gcaatctcct cctcgctcgc
     2701 cgcgcgcacc tccagcacct cgatctcgaa gccgagcgtg cgtccggcga gcggatggtt
     2761 ggtgtctacg tcggcattga acttgccgac cttcagcacc gtgacctggc gtgcgccgtg
     2821 ctcggtctcc accgcgacca ccatgcccgg gcgcagccgg ccctgcgcgt gcaggtgctt
     2881 cagcggtacc cgctggatcg agtcggcgcg gcgcacgccg taggcctgct ccggttccag
     2941 cgtgaccgag agcgtatccc cggccgcgtg gcccgcgagt tgctgctcga gcgccggcag
     3001 gatgttgccg tgcccgtgca ggtagacgac cggttcgccg ttacgcgagt tctcgatctc
     3061 ggcgccgtcc tcggcgcgca gggtgtagtg gaagctggcg acggttccgc tggcgatctg
     3121 catggtcctg gctccgcgaa aaaagggcgc aagtatcggc cacgcgcccg cctggcgcaa
     3181 cgcacgcaac gttgcacgcg cgctcagatc cggcgcagcc agaccgtgat ctcctcgcgg
     3241 tcgtggtaga gctggcgcgc cgtgcaggca acgcggccac cgccgtcctc cagttccgcg
     3301 acgatgcgcg cgagcatcag cgcggcgtcg cggtagcgct gcttcatcgg cagcttgagg
     3361 ttgaatatcg cctggcggca ccagccgttc aggaaccagc gctcgatcag ctccgccacc
     3421 cgcgccggct tgtcgacgat atcgcagacc agccagtcga ccggatgcgc ggggcgaaac
     3481 gggaaggcgt cctgcgcgag gtgacgcacc gcgcccgcgt gctgcgcctg cgggctgagc
     3541 gcgccgcgat cgaccgcggt caccgcgagc ccgcgccgtg ccagtatcca ggtccagccg
     3601 ccgggcgccg caccgaggtc caccgcgctc atgccgctgc gcagcagacg ctcggcctcg
     3661 tcacccagaa aatacagcaa tgcctcttcc agcttggccg ccgagcggct gggcgccccc
     3721 ggcaggcgca ggcgcgggat acccatcgac cagcgcgccc cgttggccac gggcgagacc
     3781 cctacgaagg ccttgctgcc cgagaggaac agcacgtgca ggcgcatggt cgcgcgcctt
     3841 gccagcagtt gctctgagcc gagcgctccc tcgaggcgcg cctgcagccg ggtgcacagc
     3901 ttgccgaccg agcgcccctc ctccgtatcg acatgctgca gccagcagtc cgcgaacggc
     3961 ccgagcgcgc gcgctgccgc cagcagcggc gcgatgcgat cgccgaccgg caggtcctcg
     4021 agcacccggc ccgcgagcca gtcgcgcgcg aagaccagtt catcgaagcg gatcccggcc
     4081 agcgcgctgc gcgcgtccca gtccccgccc gagcgcacct ccaccagccc ggtgcccgcc
     4141 tcggcgcgac accacgccgc gacgcccgtg cgggcaagac tgtcgatgat ctccgcgctg
     4201 cagtccttct cgaagccggg acggcacagc agcaccaacg tatcgggcac ggattcgctc
     4261 atgggggagg atgataaccg cagcgccacg cacgtccgct tacaggcgga caaaatagac
     4321 gcgggtccgc attcatgcgg actcttgccg gtctcaatgt ccggccgaag ccggacccac
     4381 ggcttgcagg tacgggcgca gcaattcgcg ccagtgacgg tagcccgcgc cgctgaggtg
     4441 cagctcgtcg aaggtgagct cgtcgcgcag cgagccgtcg ggcgcgagga aggccggata
     4501 gagatcgatg aagccgactc ccgcctcgcg gcagatgccg cgcagcgcat cgttgtagct
     4561 ttcgacgcgt tcgcgatagg tccccgcgcg cggcagcagg ctctgcacga agacccgcgt
     4621 atccggcgac tcgcggcgga tgcgctcgag aatctcgcgc tggcgcgcga ggctcggcgc
     4681 cggatcgagc gacggggcca gctcgttggt gccgatcagc aggaacaccg ccgcgggccg
     4741 cgatgcggtg accgtatcga gccgtgcgag cacgccggcc gtggtgtcgc cgctgatacc
     4801 gcggtcacgc accggcaggt ccgggaacag ctcgctccac tccgcgccct cggtgatgct
     4861 gtcgccgagg aacacgatgt caccggcacc cagcggcgtc gccgcgaaca ggtccatgcg
     4921 caggcggtag taagcgtcgg gcgagaaggc gtccgcgcag ccctcgcgcg gcgtggcggc
     4981 gaagcaggca acgccgaggg ccaatacgac aaccagccgg atcgagcgca tgcgccacct
     5041 cctcggtgtg tctgtctacg tgctgcgcga atggtggcgc gcagggcgcg cccgcaccag
     5101 gcatcacccg cggtgagcgc gccgcgaagg ggcgcacaac cgctgcgcgc aaacggatcg
     5161 agcgaaagcc tggcgttgcc ggaagcggtg gtggaggggc ttgcccgggg cgcggcgtta
     5221 catcacgtcc cgccgcccgg gggagacgag cctgtcaggt cgtgcgcgcc gcgcgcgctg
     5281 caggggcgac cggcacttgc ggcccaccca gcttgccgta gccgctgact cctttgggaa
     5341 tttgctggat ctcgccgcca ccggccagaa aggcagccgt cttctcggcg atgatgcgtt
     5401 ggtgctcggc cgtcggcacg gccgcgttgg cttgggtgct cgacctgctt ggttttgcca
     5461 tcaactttct cctctatcgg acctgcagtg tacgcccgat ggcggcaaaa gtcgtccgag
     5521 gcccccgggc ggccgggttg cgccccgcta ccgggccatt tgaagaggcg gatcattcac
     5581 aataattatt ttgaattcaa ttagttgaga cacctcccgg cgcgccgcgg caagcgagcg
     5641 cgccacccgg cgtcgatgga tgcgtggggt ccggacccgg gcgatggcgg gtctcgtccg
     5701 gcgtggcacg gcaaaaccgc tgccgggacg ccggccggct gctacgatac cggccgccgt
     5761 cgcattaaca gaaaaccgta ccaggagttg ccgatgacca gacccggtcg catcaagccc
     5821 ccgatggggc acgacaatgc atggtggtgg gagcaggccg ccaagggcgt gctcgcgatc
     5881 cagcgctgct ccaggtgcca ggcactgcgc cacccgccgc gcccgatgtg cggcgagtgc
     5941 cgttcgatgg agtgggacgt gctcgcggcc agcggcaagg gcacggtggc cagctacacg
     6001 gtgctgtacc acccgcagtt cccgggcttc gaatacccgc tgatcatcgt gctggtcgac
     6061 ctcgaggaag gcacccgcat cacggccgaa ctcaaggact gcgatcccga ggccgtcgat
     6121 ttcggcatgg cggtcaccgc ttacatccac gaggacgaag acggcttccg gatcccgatg
     6181 ttccggccgg ccacccgggg aggaaactga gatgccgatc agcatgaaga acgaggcggc
     6241 catcgccggc atcggtgcca cgaaattcag ccgcaactcc ggcgtgtcgg agctcgcgct
     6301 ggcctgcgag gccgtcagca actgcctgga tgacgcggga ctcgcgcccg gggaagtcga
     6361 cggcctggtg acctacacgc tcgagtccaa cgacgaggtc gaggtggcgc gcgcggtggg
     6421 cctgggcgac ctgaccatgt acgccaagat caattacggc ggcggcgcgg cggtcggcct
     6481 gatccagcag gcggcaatgg ccgtggcgac gggcgccgcg aagaatgtcg tggtgtggcg
     6541 cgcgatgaac ggccgctccg gccagcgcat gggccagggc gtttcgggca acatcatcag
     6601 cagcgacctg atccactggt cgtggtacat ggcctacggc atgctgacgc ctgcgtcctg
     6661 ggtggcgatg atcgccaata cctacatgca ccggttcggc gtcgccgccg aacacctggc
     6721 ggaagtggcc atcgcgcagc gcaacttcgc gctgaacaac ccggccgcgg cgggctacgg
     6781 caagccattg acgctgcagg agtacatggg ctccaagcgc atcaccgagc ggctgcgcat
     6841 ctacgactgc tgccaggaga ccgatggcgg cgtcgcactg ctgatcacca gcaccgagcg
     6901 cgcgcgtgac ctgcggcaca agcccgcggt cattcgcggc gtcacccagg cctcgacgcg
     6961 cggccaggag cagatgacca gcttctaccg cgaggaactc gaccgtctgc cggaaatgga
     7021 gctggcggcg cggcgcgtct acgcgatgtc cggcctcggc cccgaggaca tcgatgccgc
     7081 ctgcctctat gacgccttca cgccggaagt gatcatgcag ctggagtcgt tcggcttctg
     7141 caagcgcggc gaagccaagg atttcgtcgc cgccggcaac ctgcgcctgg gcggccggct
     7201 accgaacaac acccacggcg gcttgctgtc ggagtcttac atccacggca tcaacaacat
     7261 tgccgagggc gcgcgactga ttcgcggcac ctcctgcaac cagcccgaac gcgtcgagca
     7321 cgtgctggtg tccagtggcg tgggcgtgcc gaccggtgca ctgatcctcg ggcgcgactg
     7381 agtccgtacc gcagcctgcg gccagtgggc tgcggtcact gctcacaggt cggacaggta
     7441 ttcgatgcct gtcatggttt cggacagggt ccacagccgc ttgccgacgt cggccttttt
     7501 cgccacggga ttgacgcgag cctttttcgt ggtcttgccg ccgatctcga ggaagccgct
     7561 cgggccgtaa tagtcgccgc cactcacgcc ctgggccgtg gcggcatgaa tgatcggcct
     7621 gacggcctcc gccgcggtgg gaatcagcgg ctcgagtttt ttctggcgcc aggcgcgcaa
     7681 tccgctctgg gctttcagcc cggcgctgtt ctttgccacg tcggtattgg caaacccggg
     7741 atgagcaccc agggcgatga tgttgctgtc actcttgcgc aggcggcggt ccagctccat
     7801 cgtgaagctg agcatcgcga ccttgctcct gccgtagccc tgccattcgt tgtacggcgt
     7861 tctttcccag ttcaggtcgt ccaggtccag cttgccgaaa cgatgcatca ggctgcccac
     7921 attgacgatg cgcgcctgag tcgtcctggt gaagaacggc agtagcgtgc ccgtcagggc
     7981 aaatgctccc aggtagttgg tggccagttg catttcctgc ccgacgctgt tgcgcgccaa
     8041 tggcgtggcg acaatgccgg cgttgttgat caacaggtcc agttgcccca tctgcccgga
     8101 aaactggcgg gcgaactcct gtatcgacgc cggttccgac acgtcgagag gaatgatcac
     8161 cgccctggcg tccggcactt cggcgagtat ttcgccatgc gccttcctgg ccctgtccat
     8221 gctcctgcac gccagcacca cctgcgcacc catgcgcgag agattcaacg ccgcctgaaa
     8281 cccgatacct gcattcgcac cagtaaccac aaccactttg ttcagcacga caatctctct
     8341 caccgttgac cagaccgcaa gtaatctgcc tgcttcaggc agtgtccacg aacaccgtgc
     8401 cattctcgac cgtgaccgga aacgtacgca ccgcctgcgg ctccttgccc atcgcaacca
     8461 gcttctggct ccaccgcggc agcgcgatcc cggccaccga attcacccag tcgacattgg
     8521 cgccggtttt gacgtcgaag ctcgagccgt gccacgggca ggtgatattg ccattgctga
     8581 tggcgccctt ctccatcgac aggccaaagt gcggacacct gttctcgagc gcgaagatct
     8641 ctcccttctc cctgaccagg agaatgcgca acccgtcgag cttgacgcac ttgctcctgc
     8701 cttcggcgat gtcatccacg cgcgcggctt tcactttcat ggcgaccccc tgtggctgtc
     8761 atgtagcgag ggaaaatcgc ggacggccgg cccggccgcc cgcgccggcg ctacttcgcg
     8821 gtgcgcttgg caggcgcctt cttcgcggga gcctttttcg cgggagcctt tttcgcggga
     8881 gccttcctgg caggcgtctt cccggccgtg gcgcttgccg ccttgaccgc ctcgaccacg
     8941 gccgcttcgg ccttcttcgt ggcgcgcttg acggaagcga tgcccttgtc gagcttcatg
     9001 ccgggcgtcg ccgccttgcg cgcggcgggc tttttcgggc tcaccgcctt ctcgaccttg
     9061 acggcggcct tcttgacgcg cgccgccgcc gtcttcacct tgcgcttgac ctggcgctcg
     9121 gccttgccgg tcgccgactt cacgtcggcc ttgacggact tcgccttgcg ctcgaccgcg
     9181 atttccgcat cgtgcgccgt cttctgcacg gcgcgggccc ccttgttcac cgcgcgcctg
     9241 gcagcatcga ctgcctcgcc tatgcgaccc atcagacctt tctgcttcgt cattgcggcc
     9301 actccccttg tatcagcaaa ttgctggcga ttgatggtgc atgccggtgc tccggcacac
     9361 ccgtgcaaac aatgggcaaa gcacttcgcc catgtcaagc atcggcaccg atgccggtcc
     9421 ctgcgccagc gcgctcttcg tgcgcgcgca cgatcgcgcc gagggcgcta gcgggctgtc
     9481 aatgagcgta ccgcgtcgct catcaccttc gcggcatcct cggggagcag caaaccctgg
     9541 cgctgcagcg catccacgct ctgccggacc ctgacgagat acgacgcagg gttcgggtac
     9601 agcgcgtcga gggtcgcctg gtcgaacggc agcacggagc cgctcagccg gcacgccagg
     9661 ttgccgatgg cgagcagcgc gggcggcagg tccgggctgg ccgtgttcgt cgagaggtag
     9721 gtggcggtgg gcgcctccag ggcgggcagg cgtacaccgc cgatgacgtt gccgagcgcg
     9781 tcgcgcgcca gcacgccatc gatcgcctcc atcacctgcc ccgccggaac ccggcgatcg
     9841 acgatccggt aatagccgcg gaaatccgcg tgtgtttcca tgcgggccca gagcgcgttg
     9901 agaacatacg aggcgaacac gggtccatcc gccgtcgaat tcatcgcgct ggcgcagaga
     9961 tccgccagcc ccagcgcgtc gggcccgagc aggcccgccg gaacgagctc gatatccttg
    10021 tgcaccacgt tgtgcgccgc gcccgccacc tcgaagtagc gaaaggatgg cgtgtcgggc
    10081 tgcctgccca gcacgttgaa gttcagtgtc tcgaaatccg tctgcgtgat cacctggtag
    10141 acgggcaccg gcaggtctgt ccggaccagg gagtcggggt gagccagccg cggcgtgcac
    10201 tcaggaaacg cgggtgcacc ggcatcatcg cagcgcggac cgaagttgat cgggcgcgca
    10261 tggacgttcg actggatgaa atagccgtcg ttgacgtcat cgaggtggaa ggcgctcgcg
    10321 taggtgatca ccgaacccgc ctgctgtgac tcgcccgcat gaaacaggcg tcgtacccgg
    10381 tatgcctgcg gcagggggtt gtgcggatcg tcgctcttca gcaggtttgc gatctggctc
    10441 atcatctcgt aggccagccc gttctcgggc agcgacagtg tcgcgtagcg cgatccgcag
    10501 gacggcggca acacgccgaa cacgcggcaa ccgccggcaa gaaacgcgat ggcggtcgtg
    10561 ctgttgctga caccgacgta ggcaatgccg cggcgcgcga aatactccgc cgactggtcc
    10621 cacaccgggg cgccatcgaa tccggcggtg gagttccacc actcgatgac gacggtaccg
    10681 ttgaaggcct tcggatccac gggacggcgt acgatcagcc gcgtccggta cggcacgtcg
    10741 gcatcgatcg ccgccaggtc cgtcgggccg agcggcgggt tgtgcgcgta attgaacagc
    10801 gtcgcgtgcc cctcgacgaa gaactcctcc tcgacgtagg gcacgccggg caggtttccg
    10861 agcaggcgcg tgccgggcac gaaggcctcg cccggcactt ccggcggcag ggacacggtc
    10921 accggatccg tcggtcccgc gtgggcaagg cgcgcgccag ccggcagcag aaagagaacc
    10981 aggacgagga actgcaaaac acgcgtgctg ttcatcatgg cgttgcctcg acgagtaaaa
    11041 aaaatgcgcc gtttccagcc gcttcaatcc atgtccacgt tcaacacgag cgagcgcagg
    11101 cggttctcca tcacgcaggg ctcccactcg ggccgttgcg ccagttcgaa cgagcggcag
    11161 cgctcgacga acaccgccag cgcctcctcg atggtgtatc tcgccatcgc ctgccccagg
    11221 cagaagtgcg caccgatgcc gaaggacagg tgccacacat ccgggtcgcg gctgatcctg
    11281 aattcctcgg gatcctgcca gcgctgcggg tcgcggttgg gcgagtgcag cgggatcatg
    11341 atcgtgtcgt tggtgcggaa ggaatggccg aaggcctcga aatcgtgcgc ggccttttgc
    11401 ggcatctccg cgataccggg atgcatgcgc agcgtttcct cgcacacgcc gcgtttccat
    11461 tttcccgggt cgctgcgaaa cagcgcctgt tgctccggat gcagtgccag ttccagcaag
    11521 ccggcgtcca gggtgccggc ggtcgtgctt cccgatccga agatggtgga aaacagcgtg
    11581 atcagttccg gcatcgacag cgcgccgcga tcgtcatgcg cctcgagcag gctggtgagc
    11641 aggtcgtcgc gcggctcgcg gcgccgcttc tcgtagagcg atgccacgtg gtccatgacc
    11701 atccggatgg cgccgttggc cttgtcgcgc atcgcctgcg tgacgttctg gccgaagcag
    11761 tccgctatgc gcgcatcggc cagcggcccc atcgagccca tgagctcgtc ggcgccgatg
    11821 ccgaggaact ccatcatcac caggcgcggc aactgcgcgg tgaagccgcg ctggatatcg
    11881 acgctacccg tcctggggaa cctgtcgatc aattgaccgg cgatgctgcg gatcaacggg
    11941 cgcagcggct ccatgttgcg cctggacatc gcgccggtga ccagcttgcg gatgcgcgtg
    12001 tgatcggggc cctccatgat gccgagcgcg tggctgcgcc aggtgtgcag cggatcaccg
    12061 gggcggaaac cccgggcttc gagccgctcg atcccctcgt tgatgaaatc gtgcccgcgg
    12121 atcgcccact cggcgtcatc ccagcgcagc acggcgagct gtcccgtgtc cgtgaaggcg
    12181 gtgcgatgct tcgcgcggaa cccggcgagc accggatggg ggttttgcca gaaggccgga
    12241 tcgccccagg gaagaagctc tgccgcgctc atgtggtgct ccgttgttca gctgcttgtt
    12301 gtcttgtcgt cagcgatcga acccgatcag ggcttcattc tgccacgcgc gcgccgtcga
    12361 cgtgaaagcg cacgtagggg agctgttccc gcgcaatgcc gcccacgccc gcgggcacgg
    12421 ccgtttgtgc gccgggcgcg agcgtgccgc gcaggcgcag cggcgagccc tgctgcacga
    12481 cccgcccggc gttgttcacc agcaccggcg tgagctgcac atcgcgcagg gccaccggcg
    12541 cgcggttgcg cacgacggcg tacaaccgac cctcggcatc gaactgcccc ttcgccgtca
    12601 cgtagttgcc cggattctgc ggcaggtcca tgcgcatgaa ctccgccgcc gccgcctggc
    12661 cgatgggacc gctggatttg gcggccgcac gatagtagga catggccctg gctccgtcgc
    12721 cgcgatcgcg cgcgatgttg ccgaggtagt aggccgccgg tgccgtgggc aggagctcga
    12781 cgctgcggtt gagccactct tccgccttgg gcttgttgcc ggcctgcaac tgcgccacgc
    12841 cgccacccag ccaggaaccg aagtagcccg gattcagcgc cgtcgctttc tggtaatgcg
    12901 gtatcgcggc ctcgggccgc ttctccgcga gcgccagctc gcccatcaat tcatggaagc
    12961 ggccttcgcg cggcagcaac tggatggcct ccccggtcaa cgacctcgcc gctgcgaaat
    13021 ccttcctgcg cgccgccacg atcgccttgt cgtacttgtc gtacgccggc ttgatcgcgc
    13081 gcagcggctt catgcgcgcc gcatagcgct cggcgcccag ttccccgccg cggccgagct
    13141 gttcggcagc aaggcggttc tgggcaacgc gctcggccga gggcgggtgc gaggcgaaca
    13201 ggccctccag ccagccctgc ttcttcgcgc cgctctcgga caggcgcacg aaggtctcct
    13261 gcagggtgac cgcgccccag ggatcgtaac cggcggcctt catgtacttc atgccgtact
    13321 ggtcgccatc gagctcctgt tcgcggctgt acttctgctg gatcatctgg gccccgacgc
    13381 ccgcgccctg gatggccagg ttggccgtgt tctggtcgac gccgccgatg gccgcgccga
    13441 tctgcgccgc cgccatgcca acctgcagca gggtgccgcg ctcctgcgcc ttggcgccat
    13501 ggcgcgccgc cgcgtgcacg atctcgtggc cgagcaccgc cgcgagctcg gcctcgttct
    13561 gcaattcggt gagcaggccc ctgttgaccg cgatcttgcc gccgggcagg gcccaggcgt
    13621 tcggcaccga gctgttgagc acgacgaact cgtacggcag cttgcgatcg gccaccgccg
    13681 cgagtttctg tcccacttcg tccacatagt ccgtcagttc gggcatcacg gtgaaatcgc
    13741 cgccttcgct ctggcgggtc ggcgcgtagt actgctcgcc catcttcact tcctgggctt
    13801 cggatacgaa ctggatctcc ttgtcgcccg tcacggggtt gacgccgcag gcgctcagga
    13861 caccgaccga gaagatgcac aggcataatt tcaggcttcg catggccaac tccttcgcgg
    13921 ggatgcgggc aacgttcggc atcgtatatc cgacgacgac gcagccggtc aaatgtgccc
    13981 gcgggaaagc gacgcccgtg gactcgggat cgcgacctac gtgcaggcgc cgccgggcaa
    14041 gcgccggcac gcgctaatcc gcgccggggc atcgacacgt acaatcgtga tggcgacccc
    14101 caccggcatg aagctcgcaa ggctgccgcg aatcgcactg gtggtgctgt tcgcgctggc
    14161 gctgtcggcg tgcggcacgc gcctgctcta cgagcgcatc gataccctgc tctatttcca
    14221 gctcgccagc caggtcaccc tggacgacgg gcaggcccgc gcgctgcgcg cctcgctgcg
    14281 cgagctccac gcctggcacc gcggcgcggc actgccggcg tatgccgaat tcctcgagac
    14341 cctggcggat gacatggcgc agccggtggg cgcgggcggc atcgacgcgg cgcgcatcgc
    14401 tctcgagggc ttgtggcgcg atacggtcgc gggcgtcgca cccacggccg cacgctggct
    14461 cgcgggtctc gatcccgcgc agcgcgagga attcttcgcc ggcctggccg aacgtgacgt
    14521 ggagctgcgc cgggagtact gcgacatcgg cgacgaggag cgcaggcgca accgcgagcg
    14581 cgcggtggtt tccgcgctgg gcgactgggt gggtcgcctc ggtcgggagc agaggacact
    14641 ggtgcgcgag cagttgcggg gcatcgacgc caacggctgc gactggatcg cgagccgcgc
    14701 gggctttcgc gagcgcctgc gacagctcgt cgacacgcat gccgcgcagc cgcactacga
    14761 ggcgcgtgtg gcgcgcctgc tgatgcaccc cgaagacggc tgggacgccg cctaccgcgc
    14821 gcgctacgcg gccaacagcg cggtcgtcgt gacgatgctg gcggagctcg accgcacgct
    14881 cagcgaacgc cagcgcgcgc ggctcggcgc gaagttgcgc cgctacgccc tggattttcg
    14941 cgagctcgcc gccatctgag cggcagcact tgccgcgcca gcgccgcgct cacgaacccg
    15001 caagaccggc caggtaatca gtgatcatcc gtgccagctc ctcggtgctc cccggcgaca
    15061 ggatgtcccc cgcgagcacg tgttgcgacg ggtcggcgga gcgctcgaac acgaccagcc
    15121 gtttgcgctc cccgccccac tcggcaaacg cctcggccac cgccgccggg tcgatcaccc
    15181 ggtccgccgg cgaatagatc atcagcgtcg ggacgccgat cgcggacttg tcgagagccg
    15241 ccacctcctt cacgagccgc accagttccg gcagcgcccg gtagggataa ctccacgtcc
    15301 agtagcgggc ctggcgcgcg ttgtgcggct gccagctgcg gtagccctcg ccgagatagc
    15361 cgagcagcgc cggtccgagc aacggccagt cgagcaggta cagcgtgcgg tcacgcggcg
    15421 cgaaattggg cgagatcatc accagcgcgg ccagcgagtc gtcgtgcacg cggctcgccg
    15481 cccaggtcga cagcgtgccg cccgtggacg tcgacagcag cacgacgcgc tcgccgatgg
    15541 cgcgcccgat ggccagcgct tccagcgtgt cctgctgcca cgccgccacg gaaccctcca
    15601 gcatcgccgc gccatcgcgc ccgtggccgg tgaggcgcgt gtggaacagg ttcgcgccta
    15661 gcgcggcggc gacccgctct gcgagcggga acacttcctg gcgcgtcgcc gagtagccgt
    15721 gcaggtagac cagcgccagc ggcgtccggg ccggttcgcc gccggcccac cgcaccgcgc
    15781 gttcggctcc cggcgtgatg cccggttcga gccgctcgcg cgccgcgagg tacgcctcca
    15841 cctcggcggg cgcgccggga aacgtgccgc cacgcgccgc gaagcacacc aacagcaggc
    15901 agacgatcgc cggcaagcgc gacatggcgt ggcggcctgt cacttcatcg cggttgcatg
    15961 gcgtcgttcg cccgctgcgc aggctcgcgc gagccccgga caatgccgcg acaacacagc
    16021 cggtgcacac aacatcgcag cattcctcgc gctcccaacc gagccggcat tgttgtggcc
    16081 cgatgcgcca cgcgcaacca cgccgcatgg ggcgccatgg tcgacatcgc agtgcctggc
    16141 ctgcgtcggc cggtcgccgc agttgggcta tgctggcggg gatcgcacga gggcacgacg
    16201 tgaagattct gctggcggag gacgacaagc ggctgagcga gcggctcagg accgacctga
    16261 agcgccgcgg ctatgccgtg gacgtggcgg acaacggcat cgacgccgag cacctcggca
    16321 ccgagtggcc ctacgacgcc gcgatcctcg acctcggcct gccgcagcgc ccgggcctgg
    16381 aggtgctgcg caactggcgc cggcagggca tcgcgctgcc cgtgctgatc ctcaccgcgc
    16441 gcgacgcctg gcacgagaag gtcgagggct tcaacgccgg tgccgacgac tacgtggcca
    16501 aacccttcca tgtcgaggaa ctcctcgcgc gcctcggggc gctgatccgg cgcgcgcacg
    16561 cccagcccgc gggcgtggtg agcgccgaag gcctcacgct cgacgagacg cgccaggtgg
    16621 cgctccgcgg cgagcgggag attgcgctgt ccgggatcga attccggctg ctgcgctatt
    16681 tcatgctgca tccgggccag gtgctgtcga agtcgcacct ggccgaacat ctctacgcca
    16741 gcgacgccga ccccgagagc aacgtcatcg aggtgcacgt caaccgcctg cgcggcaagc
    16801 tcggcgccga ctgcataacg acccggcgcg ggcagggcta cgtgttcgga gccccggcct
    16861 gatgcgttcg ctgctcgcgc cattggtagc gggactcgtg ctgagcctgg ccgcggtatt
    16921 cggcgtgcag tggtcgatgt tccgcatcga gttcgatgcg atgatcgtcg agtacatcgc
    16981 ccacgagctc acccacgacg ccgaggagat tttcggttcg ctgaccgtcc tgcccagcgg
    17041 tgaaaccaga ctggcgctga cccacttcga ccccccgttc ctgcgcccgg gttccggccg
    17101 gtatttccag atcctcgtcg atgacggcac cgtgctgcgt tcgccgtcgc tggacggcga
    17161 gagcctggcc gtccccacgg tgaatcgcgg gcgccggcgc gtcacgcacg tggccggccc
    17221 gcacgagcag gacctgctga tcagcgcggt cggctacgag ttcgccgggc gccagctcac
    17281 catcggcgtc gccgccgacc tgaatcccgt ccggtcgcag ttcgagcgcc tgatgacgca
    17341 ttattcgcgg gtctcggtgc tgatgttcgc gctgctggtc gcgctgcagg tcgcaatcgt
    17401 gcgcctggcg ttcggcaccc tgcggcgggt gaaagccgac gtgggccgcc tcgagcgcgg
    17461 cgaaatcacc cagctgggcg aacgcgtgcc cgccgaggtg ctgccgctgg tgcaggagat
    17521 caatcgcctg ctggccctgc tgacgcggcg cctgcagcgc tcgcgcgagg cgctcggcaa
    17581 cctggcacac gcgctgaaga cgcccctcac cgtgctgcag cacatggccg acgacgatca
    17641 gttccagcgc gacccggagc tcgcccggca gatggcggag caggtgagca tcctgcgcag
    17701 ccgcatcgac cgggaactgc ggcgtgcccg cgtcgcgggc gggcgaagca cgggcgcacc
    17761 ggtggccgtc gcgaaggaaa tcgacgcgct ggcgcagacg atgggcaagc tccatcgcga
    17821 gcgcaacctc gacattgcct gccgcatcga cccggaggcg gcattcagcg gcgaccggga
    17881 ggatttcatg gaaatgtgcg gcaacctgct cgacaacgcc tgcaagtggg cgcgctcgcg
    17941 cgtgcgcgtc acggtgcacg acggcggcgc gctggcgctg agcgtggagg acgacggccc
    18001 cggctgctca cccggggacc tggagcggat cgcacgacgc ggcgtgcggc tggacgaggc
    18061 gaccgaaggc catggtctgg ggctcgcgat cgtcgacagc attgcctctt cctatggtgc
    18121 ggagctccat ttcgggcgtt caccggaact gggcggcttc gccgtgaccg tcagcttccc
    18181 ggggacgcaa cggcccgtcc ggggcgtgaa tcgcacagcg acaccggaca acgccaactc
    18241 atgaccgagc tccccaccct gagcgcgatg ctggcggcga tcacgcggct cgaaccgctg
    18301 ctgcgcgcgc aaatgcccgc gggccggcgt gcggcgcgcc tgcccgcgcc ggtggtggag
    18361 caactgctcc ggctcggtct gttccggttg tggatcccgc gccgttacgg tggcctcgag
    18421 atgtcgctgc cggcggcact ggccatccac gaagccgtgg cgcagctcga cggctcgacc
    18481 ggctgggccg tgatgatcgg ctccggtggc ggcctgttcg ccgcctggct cgcgccaccg
    18541 gttgccgggc gcctgttcgg cgcagccgac gcgttggtgg ccggctcggg ggcgccgaac
    18601 ggcagcgccg cgcgcgtgcc ggggggcttc cgcgtcacgg gacgctggcg ctacgcgagc
    18661 ggcgccgatc acgccagcgt gttcacggcc aactgccggg tgacggagcc cggcagcaat
    18721 ccggcgccgt tgattcgcgc gatggctttc gcgccggcgg atgtaaagat ccaccgcacc
    18781 tgggacagca gcggcctgcg cgccaccggc agtcacgata tcgaggtgcg cgacgtcttt
    18841 gtgccggagg agatgagctt ttccgtgttc acggacccgg tgctggaaaa cggaccgttg
    18901 taccgccttg cgttcgcgac actgaccgag ttgccggtga gcgcggtcgc gctcggcatc
    18961 gcgcggcgcg cctgcgagga attcgcggcg ctcgcgggat cgaaatgcgc acccggcagc
    19021 acggttgcgt tgctcggact cgacctcgtc caggcggccc tgcaccgcgc gcgcgacgtg
    19081 gtcgcgaacg cggcccgcga acagcacgcg caggctgccg aggtctggga agaggcgctg
    19141 gcaggaatcc gcccggcccc gcatgtcggg gcggcgcgca cggtggcctg cgtgcggcgc
    19201 gtgcgcggcc tggtcacggc cgtcggcgat ctggtgccgc tggccggcat gaacgcgctg
    19261 cagcgcgagg atccctttgc cctcgcgtgg caggacctga acgcggcggc ggcgcattac
    19321 tccgtatcgc cgctgcagct ggcggagcag gatgggcgcg atatcgcggc caggggccgc
    19381 tcctacgacc tgcccgtcgc gtcgtcgccg ctagcgtctt gcgacgcgcc ttcgccgacg
    19441 gggaatggcg ctgccgccgc caggccaccc gattcgggcg agtagtgctc gcggtagatc
    19501 gtgacgaagg cgagcaggct cgcgatcacg atcggcccga tgatcaggcc ggtgaccccg
    19561 tagacgctcg caccgccgat gatgccgcag aagatcagca ggatcggcat gcgcgcgcgg
    19621 ctgccgatca ggatgggctt cagcacgttg tcggccaggc tcaccaggaa gaaaccccac
    19681 gcgaacacga aaacgcccca ggcgggcgac ttcatgaaga tgaagacgcc gatcggcgcc
    19741 cacaccagtc ctgcgcccac caccgggatc atggcggcga aacccgtcat cacgccgaaa
    19801 aacaccgcca gcggcacccc ggcgatctgg tagccgatca tcgccagcag gccctgtatc
    19861 accgcggtca gcagcgcacc gcgaatcacc gccgtgactg tctggtagat gcgcagcgcg
    19921 atcgactgcg cctgcgccac cggcatcggc acggtgtcga acagccagtg caggaagcgt
    19981 tcgccgtcgc gaaagcagaa gaacagcagc accaggatca gcatcaggtt cacgacaccg
    20041 agcaggatgt tgcgggccat gacggcgccg acattggcga tcttcgcgct ggccacattt
    20101 atgttgccga gcatgaattc ctgcaggtcg aactgcgcga acaggctgat ctgtccggcg
    20161 aactcgctca gccggtgcca gtagccctgc aggaatccgg gaagcaggct ggccgaaaag
    20221 gcaccctcgg gcgactgaaa cgcgccgatc cagccacgca ccgtcgggta gagttccgcg
    20281 gactggcgca ccaccacccc gagcacgaac agtgtgggca ccagcaccag caacagcacc
    20341 gccagcgtgc tcagccccgc ggcaacaccc tcgcgcgagg gcagccggcg ctgcaggaac
    20401 ttgtggatcg ggaagcagac cagcgcgagc gaggcggccc agatgatggc gtccgagaac
    20461 agcgacagga tcagcagcag ctggtagatc aggaaggcga acgcgccgaa gaagaaccag
    20521 cgcacgatac gcccctgttc cgcacccgct ggtgcgcgcg ccgacggagt cgcgccagcc
    20581 ggtgctggcg ctggcgctgg cggcggcgca gcttcgtcga tcatggcgat accgttccgt
    20641 cgatcccgag gaacgtcatt gtagcctgcc gcgcccgggc atcgcgcgcg cccagccgag
    20701 gaaatcctcc aggatcaggt acagcgccgg caccagcagc agcgtgatca ccgtcgcgaa
    20761 cagcacgccg aaggccagcg atatcgccag cggcacgaac atcatggtcg agatgtccgc
    20821 cgtggccatc agcggcgcca gcccgatgaa ggtggtcgcg gaggtgagca ggatcgggcg
    20881 aaagcgcacc gcgccggcgc gcagcaccga ctcctccacc gccacccccg cgaggcgctg
    20941 ctggttcacg tagtcgacca gcaccaggct cgcgttcacc accacgcccg acagcgccac
    21001 gatgccgagc agcgagaaga aggcgagcgc ctggcccatg atcaggtgac ccagcagcgc
    21061 gccgatgacg ccgaacggga tcgcgctcat gatcacgaag ggctgcaggt aggaacgcag
    21121 cggcaccgcg agcagcgcgt agatgatcag cagcgccagc agcgtcatcc ccacgaggct
    21181 gcccatggcc tcggcgcgct cctccgcctc gccggcaagc gcgaaggaga tgccggggaa
    21241 tttcgcctcc aggcgcggga actcggtgtc ggccaccgac gccagcacct tttccggcac
    21301 caccacctcg cgatccacat caccctgcac gctcaccacg cgctgtccgt ccacgcggcg
    21361 tatggtggaa tgaccgcgtc cgaccgtggt ttcggccacg ctcatcgagg gaacctcgac
    21421 accctgcgct gtccggatgc gcatgttctc caggtcgccc agcgacaccc gctccgcttg
    21481 cggcagcctc accatcacgc gcacgtcgtc gcgtccgcgc tgcacgcgtt gcgcttcctc
    21541 gccgtagaac gcctggcgca cctggcggcc gaggtcgcgc tgcgacagcc cgagcagttc
    21601 ggcctcgggg cggatcgcga gcaggatttc ctgcttgccg gcgcggtagg tgtcgctcac
    21661 gtcgaacacc ccgtcgtagc gcgcgagcgt ctcgcgcagt tcggacgccg cctcgccgag
    21721 ctggtgcacg tcgcgaccgc gcagctcgat gtcgatgggc ttgccggcgc tgaaggcctc
    21781 ggccgtgaac gtcagctcca gcgcatcggg aatcggaccg gtgagctggc gccacagcgc
    21841 ggccgcctcc gccgagccca tgccgccgcg ctcggcatag gggcgcagct ccaccagcac
    21901 ttccgccacg tggctgccct cgccggcgtc cgaaccgagc gaaccgcggc cgacacgctt
    21961 gcccaccgag gccatgacgt gccgcacggc cgaaggctcc gcatcgtcac ggcgcccccc
    22021 atcgaggcgc tcgcgcagcg cctcggccga agcttccagc cggcgtgcgg cctgctgcgt
    22081 cacctcggcg ggggtcccct cgggcatggt cagcgaggca aacaggcggt cgccctcgac
    22141 cgacggaaag tactggaaca cgatgcgacc gctcgcgagc aggcccacca tgacgatcat
    22201 gacggccacg ccgatcgcgc acaccacgta gcggtgtgct atcgcgcggc gcatcagcgg
    22261 ctggtagctg tcggcgatga aacgctccag gcgcgccgat atgcggtcct gcaggcgctc
    22321 cagccagttc tcgtcgcgcg cgccggccct gcgcaccgtc tggcgggcga ggtgcgccgg
    22381 caggacgagc tgcgattcga gcaggctgaa acacaggcac agcaccaccg tcaggccgac
    22441 cgcggcaaag aacgacgcca tgccgccttc cagcgtgatt atcggcagga acgccgcctg
    22501 cgtcgtgagc acgccgaaga tgaccggcac cgagacctcg ctggcgccgc gtatggccgc
    22561 ctcgcgcggt gccatgccct cgagctcgaa ggcgtgcacg cgctcgccca cgacggtggc
    22621 gtcgtcgacg atgatgccga gcaccagcag gaagcccatc agcgacagcg agctgatcgt
    22681 gaggcctgcc gccggaaaca tcgcgagcgc accgagcatc gcgaccggca ttcccacgct
    22741 cacccacagc gcgatgcgca ggcgcaggaa cagcgcgagc accacgacca ccagcacgag
    22801 gccgctccag gcgttgccga gcagcgcatc gaggcgatcg acgagatcct gcgactcgtc
    22861 gcgcgagatg tcgatcgcca tgccctcggg cagctgcgcg cgcttgcgcc cgaggtagtc
    22921 cttgacggcg ccggcgatct ccagcgtgtc ttcctcgccg acgcgcgaga cctgcagcag
    22981 cagcgcgggc ttgccgtcgt agcgcacgcg caggtcggta tcctcgaagc cgtcgaccag
    23041 ggtcgcgatc tcgcctaacg tgagcgcggt gccgtcggcg cgcttcgtca ccacgatgtc
    23101 gcggaattcc ccgcccgtgt agcgctgccc cttggtgcgc agcaggatct cgccgcccgc
    23161 gctcttcacc agtccgcccg gcacgtcgag gctggcgtcg cggatcgcct gcgcgatgcg
    23221 gtcgaacgtg agcccgtatt gccgcagcgt gtgttccgag acctcgaccg cgatctcgta
    23281 gggccgtgcg tactggaggc gcacctggct gatgcgcgga tcctccgtga gttcctcgcg
    23341 cacctcctcg gccagctcct tcagcgcgcg ctcgtcggtg tggcccgaga tcgcgagctc
    23401 catcacggtc gtcatcaccg tgacctcgtt gatcaccggc cgctcggcct ccaccggaaa
    23461 ggtgttgatc gcatagaccc gcgcgttgac gtcatcgagc gccttgctct tgtcggcgtc
    23521 gtggtgcagc tgcaccagca ccgagcacag tccttccgcc gcggaggtca tgatcttcct
    23581 gatgccctcg gtgccctcga tggcctcctc gatgcgcagg cagaccccgg cctcgacttc
    23641 ctccggcgcc gcgccgaggt agggcacggt gatctggatg taatccagct cgaggttggg
    23701 gaactcctcc ttgcgcagct gcgacagcga gatcaggccc ccggcgatga agagcagcat
    23761 cagcagattg gccgctaccg ggttgtcgac ccaccaggca atgaggcgtt gcatcggatc
    23821 aggactccgc gtccgtgtcg ctcgcgaccg gcgccacccg catgccgtcc accaccagtt
    23881 gcagcggcga gacgcacaca cgctcgccgg cctcgatgcc ggagctgacc agcacggtat
    23941 cgcggtcggc gcgcagcagc gtcacgggac gaaagcgcag gcggtcttcg gcatccacca
    24001 ccagcacgcg gtcgccgtcg cgcagcgcgg cgcgcgggag ctcgatcacc ccgcgcgcgg
    24061 tctggccgcg gatctcggcc tgcacgaaga gcccgaccgg cagcggcgcc tgctcgggcg
    24121 cgtccgggtt ctccacgcgc gccacgacgt gcaccatgcg gctgcccgca tcgatctcgc
    24181 cctcggtgcg cacgatgcgc ccctcccagt tccagcgctg gccgccgaac tcggcatgca
    24241 ggctgaccgg cgccgcatcg ccgccggggg cgatgccgct gcgaataagc gccggatcga
    24301 ggaatgcgag ctgacggtcg gcgatcggca ggcgcacttc catgtaatcg gtagcgtaga
    24361 gcgttgcgat ccggtcgcca cggctgagcg tctgcccgat gtcgacgcgc gcgctgcgca
    24421 cccggccgcc gaagggcgcg ctgatggcgg tgcgcgcgag gtccagccgc gcgcgctcga
    24481 gggcgacctc ggcctccttc aataccgact ccgcgacgcg gcgggcgcgc gatgcgttct
    24541 cggcctgcga gcggctcgcc agctcgcgcg ccagcagcgt cgccatgcgc tcggactcga
    24601 aacgcgcgaa ttccgcctcg gcctgcgcgc gcatcagcac cgcctcggcg gcgccgacgc
    24661 cgctgcggta atcgctgtcg tcgatgcgca gcagcagctc ggcggcggcg aaacgcccgc
    24721 ccgagcgcaa cgacggcgac atccagacca cccgccccga gacttccggg atcagctccg
    24781 cctcggtgtg cggttccacc gtgccctgcg agcggacgat cagcggcacg tcgaccggga
    24841 ccgcctgcac cgtgcgcacg gcgagcagcg gacgctcgag ctgctggggc tgcagcaccg
    24901 gacgcgtggc gtagagcacg atcgcgacca ggcagcctgc cgcgatcacc gcggcggcga
    24961 gcagcagctg gcgccgacgt ccggcgcccg cggcgaaaat cggtgtctgc ggctccatcg
    25021 cggtcattcg gtttctccgt ttgccgcggc gcccgcgccg ggggcagccg gggacggctt
    25081 ccagcccccg tccaccgagc gcacgtgcag gtccatctgc gggtaggcga tctcgatgcc
    25141 cttgtcgcgg aacacctcgg caatgcgcat gtgcagatcg ttctgcacct ggaatcgcaa
    25201 cgcgagatcc tcgacgtggg cgaagcagcg catgctgagc gagctcggac cgaattcgcg
    25261 gaagatcacg ttcggcgccg gctccttcat cacgcgcgga tcggcatgca ggatctcgag
    25321 cagcgtctcg cgcaccacgc gcggatcggc gtcgtaggac acgcccacgt cgaagctgaa
    25381 ccgcaccacg gaggacgaca gcgtccagtt gatcagtcgc tcggtgacga acatctggtt
    25441 cggcacgatg atctcgcggt tgtcgaaatc ggtgatcgtg gtggcacgca tgcggatccg
    25501 gttgatcgtg ccgctgaact cgcccagcgt gatcacgtcg ccgatgcgca ccgggcgctc
    25561 gaacagcatg atcaggccgg agaagaagtt ggcgaagatc gcctgcaacc cgaagcccag
    25621 gccgaccgac aggcccgcgg ccatccactg cagcttcgac cagccgaagc cgagcatctg
    25681 cagcgcgaaa aacgccgcga gcacggcgat cacgtaacgg atcacggtct ggatcgcgta
    25741 cagcaccccc ttctcggtga tcacgctgta gaacacgatg ccgatcagcg agggtatgcc
    25801 ggatgccagc accatgccga gcgccatcac cagcagcgca ccgcccgcgt cgaacgcgga
    25861 caccgtggac acggtttcct tgccgtcgac ggtgtcggca tagctccaca gcgccacgct
    25921 gtcgaagatc gacagcgccg tgaagaactg ctgccagatc agcaccatca gcagcgccgc
    25981 cgcgcccgcg accgccacct gcagcagcgc cacggcgctc gcgttcatct tctcgaggtc
    26041 gatctcctcg aaggcatcgc cggcggcgcg cggcggcggc gcggtgccgg ccacgttcgc
    26101 cggctcctcc tgcgccaccg tatcctgtgc tgccgccagc gaacgccgcg cggcaatggt
    26161 cagcccgagc aggccggcgt cgagcagcat cttcgccgcc accagcacca cgctgctgag
    26221 gaacacgcgc agctgcaact cgcgcgccgt gaagtgatag ccctgcacgt cgagcagcac
    26281 caccagcggt tgcgcgacga cgatcgcgac cagcaggatc cagccgatgc gcgacagcat
    26341 cgggtgcccg cgggagtaga acgcggagtc gaacatatcg cgtccggcgc gcagtatgag
    26401 caccaggaag acgaagaacg cgacggtgac catcaacccg gtcacgcgat acgcctggct
    26461 cgccgccgtg gcacccgcga gcagcgccac ggtcgtcgag acgaacgcaa gcggcagcat
    26521 cacccacaac ccggcgcgca gcaggcggcg cacgctcagc acccgcgcgg tcttccagcg
    26581 caggtgtccc cgcgcgaagc cgtccgtccc catcgcctgc aggatcacca ggtacaggta
    26641 actgaggcca gcggtccggt acgaggcgtc cgacagcgcg cgcgctagcg cactgtcacc
    26701 ctccatccgt gccagcagcc agctggccca gagcagcagc agcggtaccg gcaaggcgac
    26761 cagcaggtgg atgccgaacg ccatcaggcc catgcgatag cggtagtcgc gccagcccac
    26821 gcggcgcagg ctgatcgccg cgatccgatc agccaggggc ttgcggtacc acgagaccag
    26881 caccagcacc agcagcgcga gcaacgcgct tgccgggtgc gccagcgcgt gcgtacgcag
    26941 ctggctgccg agacccgacc agtcctgcgc cgcgagcagc gccgcggtgg cgcgcggcca
    27001 ggcgatgaaa tccgcgaggc ctagcttcgg atgcgatttc atccagatca ggttgcggtc
    27061 cacgagctcg cggaacttcg cggccacctc cgactgctcg cgataggcct gctccaggtc
    27121 cacgatctgc ccggtgaaaa ccgtgtaggt gcggttgagc gcctccagca gccggttcct
    27181 ggcgctcaac aggcggtcag cctcggccac cgcggccgcg agttcggccg ccgacaatcc
    27241 cgtgtcggct tccagcgcgc gatagacggt ctcgcgcaat tgcacctccg ccgagagcag
    27301 ccgcgccacc tggaattcgc gcaggcgcgt acccgatagc aactcggcgg tggttcccag
    27361 gtcgagcgcg ggctgcccgt agctgccgag cttcgcatac tgctccatca gcaccggccc
    27421 gagcgcgtcg ctcagcgccg cgatctcgag ctgctggcgg atgctgtcgt aggcgctgct
    27481 gacctcggcg agctgcgccg tcgcgcgatc cacgtccgcg cccgccgatt ccgagcgtcc
    27541 gaccaccttg acgtactcgt cgctgagcgc ggcgatctcc tcgcgcaacg ccacgatcgc
    27601 cggatgtgcc gacccgggcc cggcgagttg cgcgcgtaca tcctcgcgca gctgttcggc
    27661 ctcgcctatg cgcttgaggt tgtccatgcc cagcaactcg gccaggaact gctcggtctg
    27721 ttcgcgctgc gcggtgacga gtttcaactc cgtctgcacc gcatgcagcc gctcgggaat
    27781 cgtgcgcgcc tcggtttcga ggcgcgcgat gcggctggcc ttcagctcgc ggtcgaccag
    27841 ccactggtcc atgcgcgcct ggctcatcgc ctcgctctcg ccggccaccc tggcaggcgc
    27901 cttggcgggg ggttcggttg cgctgagcgt ggccttcagc tcctggcgcg acgattccac
    27961 ccgtccctgc aggctgttcg cgctgttgtt cagggaggtt tcccgggcgc gcagctccgc
    28021 gagccgggaa cgggtctcgc gcagcagccg ctccaggcgc tcgaccgcca caccctggcc
    28081 gagccccttg gcggagcgcg cctggcgctg caggtcgttc agcccggccg tgaagcgcgc
    28141 gaccttggcc ggggactcct ggaacagccg ctcgtagcgg gcctggttct cgcggttttc
    28201 cgcgaccgcg tgcaggttct gcacgttctc gagatagatc tcgatcagtc gcgcgttctg
    28261 cgggtcctcg gccgagccgg cgcgatagac ctcgagctga cgctccatgg cctcgagcgt
    28321 cggcgtcgcg accgcggcgt cggcgctggc tacgctggcg gcgctcgcgc caggcacgct
    28381 cgtgcaagcc agcagcagga gcaatagggg gcgcagcgcg cgggcgcggc aaaacgactg
    28441 gaacatgggg cgggctcgtc gctcgaatgg ggtcacaagg gccggaattc tagcgcaacg
    28501 gcccgccccc atgcgcgccc cgccggtagc ggcgcacatc cgcccggcgc ggacggcatc
    28561 cgccgctgcg cgcgacgcgt atactgcgcg ctgatcacgg aggttggcat gaagcgcagg
    28621 atcatcgtcg gcatcagcgg cgccagcggc gtcgtctacg gcgtgcgcct gctgcagcaa
    28681 ctgcgtgcgc tgggcgatgt cgagacgcac ctggtcgtga gcgaaggcgg cgcacgcacg
    28741 ctggccgtcg aaaccgactg gcgcccggac gacctgcgcg ggctgtgcga cgtctggcac
    28801 cgccacgaca acctcgccgc ctcgatcgcc agcggctcgt tcctcaccca cggcatgatc
    28861 gtggcgccgt gcagcatccg cacgctctcg ggcatcgcga actgctacgc cgagaacctg
    28921 atgctgcgcg cggccgacgt caccctgaag gagcgccgcc ggctggtgct gatggtgcgg
    28981 gagactccgc tgcacaaggg ccacctcgaa ctgatgctgc gcgccaccga ttacggcgcg
    29041 cagatcctgc cgccctcgcc ggccttctac cacgggcgcc agaccctgga cgaggtgatc
    29101 gaccagaccg tgggccgcgc cctcgattgt ctcggcctgc agcacgaact ggtgccgcgc
    29161 tggggcccgc ccgcctgatc cctcgtggtg tcgggcccgc ccacttttga tggacgatcg
    29221 ctgtcaatga aatgagggag tacctgttgt aatttatcgg tcgggggtta tgctgatttc
    29281 gcttgaacac ccggaacggg tcgtctggca cggacccaac caacgagaat ttggaggtaa
    29341 gcaatgcaac tcaaaggttc caagaccgca aagaacctgg ccgacgcctt cgccggcgaa
    29401 agccaggcca accgtcgcta cctgtacttc gcggcaaagg ctgacgtcga aggcgcaccg
    29461 gacgtggcag cggtattccg ctcgaccgcc gagggcgaga ccggccacgc gcacggccac
    29521 ctcgaatacc tcgagcaggt cggcgatccg gccaccggcc tgccgatcgg c
//
LOCUS       DAORUQ010000018        29258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1145_length_29258_cov_6.179331, whole genome shotgun sequence.
ACCESSION   DAORUQ010000018 DAORUQ010000000
VERSION     DAORUQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29258
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_29258_cov_6.179331"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLW13_03115"
     CDS             complement(<1..301)
                     /locus_tag="PLW13_03115"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQY69388.1"
                     /translation="MPRALKLFLHRLQLLILQLTISVMSGRKPILLAGPGSAQRLCAT
                     VVELGARRVLIVTDAVLVKIGLIAPLAQALEAAGAACAVYDGVEPDPTHAQIEAGV"
     gene            complement(423..2336)
                     /gene="htpG"
                     /locus_tag="PLW13_03120"
     CDS             complement(423..2336)
                     /gene="htpG"
                     /locus_tag="PLW13_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQY69389.1"
                     /translation="MTVAHKETMGFQTEARQLLHLMIHSLYSKKEIFLRELISNASDA
                     ADKLRFEALSNPALLADDPNLEIRIAIDKDARTLAITDNGIGLSREEAISHLGTIARS
                     GTAQFVQQLSGDQRKDSQLIGQFGVGFYSAFMVADRVEVFSRRAGLEAAAGVRWESAG
                     ESEFSVEDIERAERGTTVVLHLKEEAGEFLDAWQVRSIVKRYSDHISLPVLMLKERFA
                     EEGKDEDKDAAPEWETVNQATALWTRAKAEVTEEEYREFYKHISHDFEDPLTWSHNRV
                     EGKYEYNSLLYIPSHAPFDLWNREAPRGLKLYIQRTFIMDEAEQFLPMYLRFVKGVVD
                     SNDLPLNVSREILQKSAAVDAMRGALSKRVLDMLDSLAAEKPEDYQKFWDVFGQVMKE
                     GPAEDFANRERVAKLFRFATTHTDKPAQDQSLAQYVERMREGQKDIYYVVADNFLTAR
                     NSPHLEVFRKKGIEVILLHDRIDEWMIGYLGQFEGKQLKDVARGDLDLGELEDSASKQ
                     AREKQQEAAKDLVERVQKALETRVHEVRVTGRLTESPACLVVGDHDMGVQMRRIMQAA
                     GQQVPEAKPIMELNPDHPLVKRLDAEPDEDRFATLATILFEQAQLASGDELEDPAAYV
                     QRLNRLLLELSGG"
     gene            2608..3186
                     /locus_tag="PLW13_03125"
     CDS             2608..3186
                     /locus_tag="PLW13_03125"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69390.1"
                     /translation="MSAPPRRRPGGRNAAVRLAVAHAVLSYLEQGQPDFSVADVAARA
                     GVHRATVYRRWPSRAALLGEALSLQYSRVTVPMTGDFAADLRTLSHRLAHYLAEPVLR
                     TVNGMAIADSEPGFGALVNTHWEPVKQRMTEMIEAGIRSGQLRRGTDPEAIIRLLVGP
                     LLMTPLFTQAGATQAQINQTADAILRAFLARP"
     gene            complement(3227..4027)
                     /locus_tag="PLW13_03130"
     CDS             complement(3227..4027)
                     /locus_tag="PLW13_03130"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HQY69391.1"
                     /translation="MSNTAGGYTAALDPRYGSGCYRRTIVLRQSGPSRVEAAVEDDPH
                     AFAITLEHDGERVTAVAAEAHRYPLTTCNGATAALQSVVGAPLSASIIELKRHADARR
                     NCTHLFDLAALAIAHVFRAARERVYRIEVPDEIGGLTEARLDRDQGRVLTWSLRHGVI
                     TEPARYAGQRVLGGFTSWAVANLAGEELEFALILQRGYFVALSRIYDMASVSMGPASE
                     DPMPSGICFSYSPGQAEHAWRVPGSRRDFSDAPEQMLRWYSPSGAGYS"
     gene            complement(4041..4913)
                     /gene="sucD"
                     /locus_tag="PLW13_03135"
     CDS             complement(4041..4913)
                     /gene="sucD"
                     /locus_tag="PLW13_03135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQY69392.1"
                     /translation="MSILVNKDTKVICQGFTGSQGTSHSKQAIEYGTKMVGGVTPGKG
                     GQTHLGLPVFDTVADAVAATGADASVIYVPAPFCKDSILEAANGGIKLIVCITEGVPT
                     LDMLEVKVKCDELGVRLIGPNCPGVITPGECKIGIMPGHIHKPGRVGIVSRSGTLTYE
                     AVKQTTDAGFGQSTCVGIGGDPIPGSNFIDILGMFQKDPATEAIVMIGEIGGSAEEEA
                     AAFIKANVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFAALEAAGVKTVRS
                     LAEIGSALKAITGW"
     gene            complement(4918..6081)
                     /gene="sucC"
                     /locus_tag="PLW13_03140"
     CDS             complement(4918..6081)
                     /gene="sucC"
                     /locus_tag="PLW13_03140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQY69393.1"
                     /translation="MNLHEYQGKQLFAEYGLPVSKGYAAETPRQAVEAAEQIGGNMWV
                     VKAQVHAGGRGKAGGVKLVKTKDEIRDFAQHWLGRNLVTYQTDEKGQPVSKILVEACT
                     DIAEELYLGAVVDRSSRRIVFMASSEGGVEIEKVAHETPEKIFKATIDPLTGAQPYQG
                     RELAFKLGLKGDQIKQFVNIFMGLAKLFQDKDLALIEVNPLVITTAGNLHCLDAKLGV
                     DSNAMYRQKGLQEMHDPSQEDPREVHAAQWEINYVALDGDIGCMVNGAGLAMGTMDIV
                     NLHGGRPANFLDVGGGATKERVSEAFKIILSDASVRAVLVNIFGGIVRCDLIAEGIIG
                     AVSEVGVKVPVVVRLEGNNAELGSKMLAESGLNIIAAKSLTEAAQSVVAAAKG"
     gene            complement(6225..7670)
                     /gene="lpdA"
                     /locus_tag="PLW13_03145"
     CDS             complement(6225..7670)
                     /gene="lpdA"
                     /locus_tag="PLW13_03145"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQY69394.1"
                     /translation="MAQQFDVVVIGAGPGGYVAAIRCAQLGLKTAVIEKWRSGDGKPV
                     FGGTCLNVGCIPSKALLDSSHKFHEAGHGLAVHGIHTGELTLDLEAMQKRKRQIVGQL
                     TGGIAGLFKANGVTALSGSGRLLAGRKVEFTPAGGAPEIIDASHVILATGSVPIEIPA
                     APFGELIVDSAGALEFTEVPLRLGIVGAGVIGLELGSVWARLGAEVVVIEALPEFLMA
                     ADQQVAKDALKLFGKQGLDIRLNARVTATQVTGDGKPMVSVSYVEGDTEKQEVFDRLI
                     VAVGRRPVTEGLFAPDSGVNLEERGFVHVNEFCQTDMPGVYAIGDIVRGPMLAHKAME
                     EGVMVAERIAGRKTQLNYDCIPSVIYTHPEIAWVGANEQQLKAAGTAYNVGVFPFAAN
                     GRALAANDSAGMVKLLIEAETDRVLGCHVVGASAADLVQQAVIAMEFGASAEDLALTV
                     FSHPTLSEGVHEAALAALGHAIHIPNRKPRK"
     gene            complement(7958..9205)
                     /gene="odhB"
                     /locus_tag="PLW13_03150"
     CDS             complement(7958..9205)
                     /gene="odhB"
                     /locus_tag="PLW13_03150"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQY69395.1"
                     /translation="MSIDIKAPQFPESVAEGSVASWHKRPGEACRRDEVVADIETDKV
                     VLEVVAPADGAIAEILKDTGATVLSQEVIARFVAGATAAAPDSAAAPAAEAAAPAAEA
                     GVAGPAARLAAAERGVDPAQVKGSGKGGRVTREDVLAHAPQAPVPAAASTPSAAAPAA
                     ARAPADLPSTPAAALVARSEQGRVERRVPMTRLRARVAERLLDASRNTAMLTTFNEVD
                     MQPVMDLRAKYKDQFEKRHNGTRLGFMGFFVRASVEALKRFPVVNASIDGNDVVYHGY
                     QDIGVAVSSDRGLVVPVLRDADAMSFADIEAKIREYGLKARDGKLAIEDITGGTFTIT
                     NGGVFGSLLSTPILNPPQTAILGMHKIQDRAVVVNGQVVVRPMMYLALSYDHRLIDGK
                     DAVQFLVAIKDMIEDPARILLDL"
     gene            complement(9239..12094)
                     /locus_tag="PLW13_03155"
     CDS             complement(9239..12094)
                     /locus_tag="PLW13_03155"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQY69396.1"
                     /translation="MQQGTMELLWRSSHISGGNAVYVEAMYEAYLRDPNSIAAQWRDY
                     FDRLPQVDGTRVDVPHSTVQQQFALLARQKARPDAPIVHPGISTEYERKQVRVVQLIS
                     AYRQRGHQKASLDPLGLAAREHVPDLDLHFHQLSEADFDTVFQTGSLYIGKDEATLRE
                     IVDALERTYCHTVGAEFMHIVSTEERHWIQQRMDGVRSAPGFEAAVKLHLLERLTAAE
                     GLEQFLASRYPGAKRFGLEGGESLIPMLDELVQRSGGYGAKELVVGMAHRGRLAVLVN
                     IFGKNPADLFDEFEGKATYQGSGDVKYHQGFSSNVMTAGGEMHIALAFNPSHLEIVAP
                     VVEGSVRARQDRRGNVERDRVVPVIIHGDAAFAGQGVVMETFQMSQTRGFCTGGTIHI
                     VLNNQVGFTTSRREDARSTEYCTDVAKMVQAPIFHVNGDDPEAVLFVTQLAADYRQQF
                     RKDVVIDLVCYRRRGHNEADEPAATQPMMYQKIRNHPTTRTLYAQRLIAEGVISAAQE
                     QELIDGYRHALESGQHVAKSLVTEPNTKLYVDWRPYIGHATTASADTRLDLATLKDLG
                     ARMCALPEGFVLQRQVQKIYEDRRLMSAAATPVNWGFAELLAYASLLEEGCLVRLTGQ
                     DVGRGTFSHRHAVLHNQNDGSVHVPLQHLGAAQAPFDIHDSLLSELAVLGFEYGYATT
                     TPNALILWEAQFGDFANGAQIVIDQFITSGEHKWARLCGLTLLLPHGYEGQGPEHSSA
                     RLERFMQLCAEHNIQICVPTTPAQIYHLLRRQAVRKLRKPLVVFSPKSLLRHKDAVSE
                     LTELAEGSFRTVIGETDAIDPESVTRVVFCSGKVYYELRDRRNAEDLQHVAIVRIEQL
                     YPFPQQELLATIAPYRQLLEVSWCQEEPQNQGAWFSSQHHMRQVVREHNRDLYLEYAG
                     REASAAPAAGYSALHLAQQDKLISDALGMQVPRWPNP"
     gene            complement(12284..12988)
                     /locus_tag="PLW13_03160"
     CDS             complement(12284..12988)
                     /locus_tag="PLW13_03160"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HQY69397.1"
                     /translation="MLQVSVYRYNPETDREPSMQELQVDTGGKDLMVLDVLELLKAQD
                     PSLTYRRSCREGVCGSDGLNMNGKNGLACITPLSETVKNERLVIRPLPGLPVVRDLVV
                     DMTMFYQQYEKVKPYLINDQPAPAIERLQSPEDREKLDGLYECILCACCSTACPSFWW
                     NPDRFIGPSGLLQAYRFLIDSRDTATNERLAELDDPYSVFRCHGIQNCVSVCPKSLNP
                     TKAIGHIRNMLLERGT"
     gene            complement(13004..14776)
                     /gene="sdhA"
                     /locus_tag="PLW13_03165"
     CDS             complement(13004..14776)
                     /gene="sdhA"
                     /locus_tag="PLW13_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HQY69398.1"
                     /translation="MARIRTLSFDGVIVGGGGAGMRAALQLAQSGYRTAVISKVFPTR
                     SHTVSAQGGITCAIASADPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSVGPQAVFEME
                     HLGLPFSRTEEGRIYQRPFGGQSKDYGRGGQAARTCAAADRTGHALLHTLFQANLKSK
                     TAFLNEWFAVDLVKNQDGAVVGVIAICMETGETVYVKSRATVLATGGAGRIYASTTNA
                     HINTGDGVGMALRAGFPVQDIEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKNGERFM
                     ERYAPNAKDLAGRDVVARSMVLEIIEGRGCGPEGDHVFLKLDHLGEDVLNSRLPGICE
                     LSKAFAHVDPVKEPIPVVPTCHYMMGGIPTNVHGQALTQDAGGNDQVIPGLFACGEVA
                     CVSVHGANRLGGNSLLDLIVFGRASGLFIEKALREGIDYRDAGDTDVDAAMSRLNRLN
                     SSTAGESVASLRKELQTVMQNFFGVFRTGEYMRHGVKALAELRPRIENVVLTDKTNAF
                     NTARFEALELQNLLEVAEATAIAAETRTESRGAHAREDFPERDDENWLCHSLYLPGDK
                     RVGKRGVNFKPKTVETFEPMKRTY"
     gene            complement(14780..15148)
                     /gene="sdhD"
                     /locus_tag="PLW13_03170"
     CDS             complement(14780..15148)
                     /gene="sdhD"
                     /locus_tag="PLW13_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HQY69399.1"
                     /translation="MVRAVTSFGRSGLSDWLLQRFTAVVLLAYVLCVGGILLFTPGID
                     YATWREHFDGTAMKVFSLMALLSIVVHAWIGLWSVSTDYLTVRMLGAKANGIRIVFQM
                     AYAVVLFAYLVWGVQILWGN"
     gene            complement(15142..15516)
                     /gene="sdhC"
                     /locus_tag="PLW13_03175"
     CDS             complement(15142..15516)
                     /gene="sdhC"
                     /locus_tag="PLW13_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HQY69400.1"
                     /translation="MKPNRPVNLDLGTIALPLPAKASILHRVSGVALVVAVAVLLYLF
                     DLSLADEAGFARAGELLATTPAKLVIWGVLAALIYHCVAGVKHLLMDLGIGETLQGGI
                     TAARITFAVAAVLIVLAGVWVW"
     gene            15843..17135
                     /gene="gltA"
                     /locus_tag="PLW13_03180"
     CDS             15843..17135
                     /gene="gltA"
                     /locus_tag="PLW13_03180"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQY69401.1"
                     /translation="MTGKTARLTIQGAAADLELPVYSGSMGPDVIDVQGLTPKGYFTY
                     DPGFVSTAACESKITFIDGDKGVLLHRGYPIEQLADKSDYLETCYLLLYGELPSAAQK
                     QRFVNVVMRHTMVHEQLQFFYRGFRRDAHPMAIMCGVVGALSAFYHDALNITDEEHRE
                     IAAFRLIAKMPTIAAMSYKYSIGMPFMYPDNTLDYSANFLHMMFGNPCEKSVINPVLA
                     RAMDRIFLLHADHEQNASTSAVRLTGSTGANPFACIAAGIAALWGPAHGGANEAVLTM
                     LEQIGDESRIPEFIAKAKDKNDPFRLMGFGHRVYKNFDPRARVMKQTCDEVLAELGLE
                     KDPLFRIAQKLEQIATEDEYFVEKKLYPNVDFYSGIILRALGIPTNMFTVIFATGRTV
                     GWIAHWHEMISASYKIGRPRQLYTGYTPRDYVPMDRRG"
     gene            17320..18054
                     /locus_tag="PLW13_03185"
     CDS             17320..18054
                     /locus_tag="PLW13_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HQY69402.1"
                     /translation="MRALAEIGSYFGIRPLLDRVVPRGDGHPVLVLPGFMASDTLTQG
                     LRDFLQRLGYEVHAWELGRNPGLRLDLCEKLEARIEAIRGASHRRVSLLGWSLGGMYA
                     RVIAQRRSDDIRQVITLGTPFRVSAEDGGKGAIARMYMKLNPDASADPLMASGLARLP
                     TPVPSTSIYSTRDGIVPWASCVEQCDARHENIRVHGSHCGMTYNPLILAIVAERLGQR
                     EHAWRPFRPSGLKAILSRPVCDPVTA"
     gene            complement(18094..18804)
                     /gene="phoU"
                     /locus_tag="PLW13_03190"
     CDS             complement(18094..18804)
                     /gene="phoU"
                     /locus_tag="PLW13_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQY69403.1"
                     /translation="MASEHHISHQFDAELEAIKSRVLAMGGIVERQIEDAVIAIIDAD
                     SQRADEVVAREKQVNGLEVAVDDEATKILVKRQPAASDLRLVLIITKAVRDLERIGDE
                     AKKIAKLALKLTEEGPAPRGYFELRHIGAAVSEMLHDALDAFTRLDVDAALEVVRRDK
                     TVDQEYRSATRELVTYMMEDPRSISRVMNIMWALRSLERIGDHSRNIAEYVFYLVKGQ
                     DLRHVGLENIESTVKSWK"
     gene            complement(18821..19645)
                     /gene="pstB"
                     /locus_tag="PLW13_03195"
     CDS             complement(18821..19645)
                     /gene="pstB"
                     /locus_tag="PLW13_03195"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQY69404.1"
                     /translation="MVQNTMTSAMPAAGETVGEAFVEAPKMRLRDVEVFYGENQAIHR
                     VSLDIAPNQIIAMIGPSGCGKSTFLRCLNRMNDTIEGCRVSGTLQLDGEDIYDPALDV
                     VQLRARVGMVFQKPNPFPKSIYENVAYGPKIHGLASRRSELDEIVEKSLRRAGLWEEV
                     KERLDKPGTGLSGGQQQRLCIARTIAIDPEVILMDEPCSALDPIATARIEELMAELRE
                     NFTIAIVTHSMQQAARVSQRTAYFHLGRLVEIGSTDQMFTTPQHKLTEDYITGRFG"
     gene            complement(19682..20986)
                     /gene="pstA"
                     /locus_tag="PLW13_03200"
     CDS             complement(19682..20986)
                     /gene="pstA"
                     /locus_tag="PLW13_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQY69405.1"
                     /translation="MSSPGGSPAPRRNTIDIVRSGLKRRYRRERRFRLYGIASVLVAL
                     ASLALLFTDIVLKGWQAFEQTQLQLEVHYDPEVLGIAPGASDEALAAADYEGVLKQTL
                     LARFPEVTARAHKRELFRLLSAGAGYELRDRLMAQPALLGRTETLWLVAHGKVDAYVK
                     ETGRIGDPPATGISAQQAAWLGQLDGAGQLDLRLNRNLLGNGDSRDPELAGIRGAVMG
                     SLYTLAVTLLLSFPLGVAAAIYLEEFAARNRWTDLIEVNINNLAAVPSIIFGLLGLAV
                     FISLFGMPRSAPLVGGLVLTLVTFPTVIIASRAAIRAVPPSLREAALGLGASRVQMVL
                     HHVLPNALPGMLTGAIIGMARALGETAPLLMIGMVAFIVDVPRSLTDSATVLPVQIYL
                     WADSPERGFTALTSAAIMVLLGFLVIMNALAVFLRNRFEKRW"
     gene            complement(20979..22355)
                     /gene="pstC"
                     /locus_tag="PLW13_03205"
     CDS             complement(20979..22355)
                     /gene="pstC"
                     /locus_tag="PLW13_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQY69406.1"
                     /translation="MNVSMLLLLIAALAVAGYYQGSSRAVSVAVPLGGVRRLPSLPAH
                     YGAYVALMVLLPALAAVLVWSLASAPLIERLVLESLPASHAGTDAGLLLNSIANLAAG
                     GATGATDPVLVSAAERMVSLGQRSSMGMWTQAVACALLGGFRGWHRVRADFVARNVVE
                     RIFTRLLFACACVAVLTTIGILLSVVVESLRFFQMVSPVEFFFGLEWSPQIALRADQQ
                     GSSGRFGAVPLFTGTLLISAIALLVAVPVGLMSAIFLSEYASAKLRSRLKPLLEILAG
                     IPTVVYGFFAALTVAPLVRDFGAAIGLDASSESALAAGLVMGVMIIPFVSSLSDDILH
                     AVPLSLRDGSLALGATRSETIKRVVIPAALPGIVGGVLLAASRAIGETMIVVMAAGLS
                     ARLSANPFESVTTVTVQIVTLLVGDQEFDSPKTLAAFALGLVLFVVTLALNIFALHIV
                     RKYREQYE"
     gene            complement(22439..23479)
                     /locus_tag="PLW13_03210"
     CDS             complement(22439..23479)
                     /locus_tag="PLW13_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY69407.1"
                     /translation="MKKLLSTAIAVAALTQVPVALAQRDNISIVGSSTVYPFSTVVAE
                     RFGRAGTFKTPKVEATGTGGGIKLFCSGVGVQHPDIANASRRMKDSEFADCAKNGVTG
                     ITEVVIGFDGIVIANARSAHHYELTLKDMFLALAKDVPNPDGSETLVPNPYKTWKDVN
                     PALPANRIEVLGPPPTSGTRDAFVELAMEGGCKSFPWVKAIKDQDENRFKAVCHAVRE
                     DGAYIEAGENDNLIVQKLGANPNSLGIFGYSFMEENADKVQGSKVAGVEPTYESIADG
                     SYAVSRPLYFYVKTAHADLIPGIREFVAEFVSERAMGEDGYLADRGLIALPQDQYEAT
                     AAAARSLAPFAL"
     gene            complement(23579..24889)
                     /gene="phoR"
                     /locus_tag="PLW13_03215"
     CDS             complement(23579..24889)
                     /gene="phoR"
                     /locus_tag="PLW13_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HQY69408.1"
                     /translation="MPTSPGRDLLLLAALMLAGLLLGDVSGNAGAGLACGVLVYLLIS
                     YRRMAKLQRWLADGPLERDPPVQGGMLGDVADRMQHTHARLQKQLEEADAAVLRLRES
                     YAALKDGIVMLGPDNTIEWSNASALTLLGLRYPQDAGQPLGNLVRDPDFTAYLAASDF
                     EHSFELEIGGAAARTLEVQATSFGANRKVIFIRDVTALNRLETMRRDFVANISHELRT
                     PLTVIAGYVGTLEDLLPQDSPVIAKVLGQMQQQSARMEHLLRDLMLLSTLEGTESADS
                     SAEESIAVCAMLDSIRENALAACGGERQITLECAPGLRLRGPRLQIESIFANLVFNAV
                     KYTGAGGKIRVVFEAGGGHALFRVSDDGIGIDPVHIPRLTERFYRVDKSRSAESGGTG
                     LGLAIVKHALKRVEGELQIESRPGAGSTFTCRFPLARLSGTAQG"
     gene            complement(24892..25611)
                     /gene="phoB"
                     /locus_tag="PLW13_03220"
     CDS             complement(24892..25611)
                     /gene="phoB"
                     /locus_tag="PLW13_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HQY69409.1"
                     /translation="MNGKQTILVVDDESSIRDMVQLSLELAGFNCLQAANAREAMGQI
                     LDARPDLVLLDWMMPDTSGYELLRRLRKDENCARLPVIMLTAKVEEGNRVQGLDGGAD
                     DYVSKPFSPRELIARIRALLRRSAPVEEQQPMEFDGLRLDPLAHRVFADARPVEMGPT
                     EFRLLQFLMSHPDRAYSRAQLLDQVWGANVYIDERTVDVHVRRLRKALEPPVTGAKDF
                     GEFIQTVRGTGYRFSNQMSRA"
     gene            complement(25666..27915)
                     /gene="rlmKL"
                     /locus_tag="PLW13_03225"
     CDS             complement(25666..27915)
                     /gene="rlmKL"
                     /locus_tag="PLW13_03225"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HQY69410.1"
                     /translation="MESAKPRIGSRPAQRHGHFFASCSRGLEELLAAELKAAGVDDTR
                     VGAGGVYITGPLAHAYRACLWSRVASRVLVAVGEVGARDADELYANAVELPWEQHIGA
                     GATIAVDFIGVSEQLRNSLFNARRVKDAVVDRLRAVTGLRPDVDVRAPDIRIVARLNR
                     ERLTLAIDLGGAQHRRGYRGATGAAPLKENLGAALLLRCGWPELAAAGGALYDPMCGS
                     GTLLIEGALIAAGAAPGEWRDLGRQKWPGHDPLLWQRLVQEARAAREQGLPGLPPIAG
                     ADADRRVLAHALDNAAAAGLAGRIEFSAVALAAQRRPEAMAGRTGLLICNPPYGERLG
                     EVRELQGLYEDLGRLVREEFPGWRAAILTTEGPLEAALGLRFARRYRFRNGAIDCRLL
                     VHDPALSTRATRRAEAAGDAEAGEPAQQTEAVPAGPLSAGAEMFANRVRKNLRRIKGW
                     VATQQPQCYRIYDADIPEYAVAVDRYGAWAHVAEYAPPAWVDEALARERLEEVRAGLA
                     SVLQLPPGRIVLKTRSRQRGTQQYQRLARENHFMEVAEGPARLLVNLRDFLDTGLFLD
                     HRPLRRMVAAEARGKRFLNLFCYTAAVSVFAGLGGARATTSVDMSATYLDWSRRNLAL
                     NGLPEAQHELVRADCLRWLGAERRRWDLVFLDPPSFSNSKRMEETLDVQRDHVALIRA
                     ALRVLEREGTLLFSTNRRGFRLDAGALADLEISDLTEQSIDPDFNRKPVPHRLYRIRH
                     RLARVTETS"
     gene            complement(27929..28957)
                     /locus_tag="PLW13_03230"
     CDS             complement(27929..28957)
                     /locus_tag="PLW13_03230"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQY69411.1"
                     /translation="MPYSLLRRLLFLLPGERSHTVSLRALDALHGLHLDALIASRPAP
                     DPVRCMGIEFPNPVGLAAGLDKNGDHIDALAALGFGFLEIGTVTPRPQPGNPQPRLFR
                     IPQARAIINRMGFNNLGVDYLCARVREAEYQGVLGINIGKNKDTPLERAAGDYLLCLE
                     RVYELASYITINLSSPNTPGLRDLQFGEPLEQLLAQLVARREALAEQHGRRVPLVLKI
                     APDLAPEDIDRVADSARRARMDALIATNTTVSRQGVEGLPNATESGGLSGAPLTRQAN
                     AVMTRLADRLAGELPLIGAGGIMTGEDAAGRVRAGASLVQLYTGFIYGGPALIGTAAA
                     AIARGRAG"
BASE COUNT         4515 a        10128 c         9872 g         4743 t
ORIGIN      
        1 cgacgccggc ctcgatctgc gcgtgcgtgg gatccggttc gacgccgtcg tagaccgcgc
       61 aggccgcgcc cgccgcttcc agcgcctgcg ccagcggcgc gatgagcccg atcttcacca
      121 gcaccgcgtc ggtgacgatc agcacgcggc gcgcgcccag ttcgaccacg gtggcgcaca
      181 ggcgctgcgc cgagccgggc ccggcgagca ggatgggttt gcgccccgac atcacgctga
      241 tcgtgagctg caggatcagc agctgcagcc ggtgcaggaa cagtttgagc gcgcgaggca
      301 tggcgaatct ccgctgcgtg aaggcgcccg gtcgcgtgcc gaccccgatc cggtcaatgc
      361 ccgcgtgcgc cgggagcctt ataaaccgtc cgtccggaag gcggcaagcc ccgcgcggtg
      421 cgtcagcccc cgctcagttc cagcagcaga cggttgagcc gctgcacgta ggccgcgggg
      481 tcctccagct cgtcgcccga ggcgagctgg gcctgctcga acaggatcgt ggccagcgtg
      541 gcgaaacggt cctcgtcggg ctcggcgtcg aggcgcttca ccagcgggtg atcggggttc
      601 agctccatga tcggcttggc ctcgggcacc tgctgcccgg cggcctgcat gatgcggcgc
      661 atctgcacgc ccatgtcgtg atcgccgacg accaggcagg ccggtgactc ggtgagccgc
      721 ccggtgacgc gcacctcgtg cacgcgcgtc tccagcgcct tctgcacgcg ctcgaccagg
      781 tccttcgcgg cttcctgctg cttctcgcgc gcctgcttgc tggcactgtc ctcgagctcg
      841 ccgaggtcga gatccccgcg cgccacgtcc ttcagctgct tgccctcgaa ctggccgagg
      901 tagccgatca tccactcgtc gatgcgatcg tgcagcagga tcacctcgat gcccttcttg
      961 cggaacacct cgaggtgcgg gctgttgcgc gcggtcagga aattgtcggc caccacgtag
     1021 tagatgtcct tctgcccctc gcgcatgcgc tcgacgtact gcgcgaggct ctggtcctgc
     1081 gccggcttgt cggtgtgcgt ggtggcgaag cggaacagct tcgcgacgcg ctcgcggttg
     1141 gcgaagtcct cggcggggcc ctccttcatg acctggccga acacgtccca gaacttctgg
     1201 tagtcctcgg gcttctccgc ggcaaggctg tcgagcatgt cgagcacgcg tttggacaac
     1261 gcgccacgca tcgcgtccac cgccgcgctc ttttgcagga tctcgcgcga gacgttgagc
     1321 ggcaggtcgt tggaatccac cacgcccttc acgaagcgca ggtacatcgg caggaactgt
     1381 tccgcctcgt ccatgatgaa cgtgcgctgg atgtagagct tgaggccgcg cggcgcctcg
     1441 cggttccaca ggtcgaaggg cgcgtgcgag gggatgtaca gcagcgagtt gtactcgtac
     1501 ttgccttcca cccggttgtg gctccaggtg agcggatcct cgaagtcgtg cgagatgtgc
     1561 ttgtagaact cgcggtattc ctcctcggtg acttccgcct tcgcgcgcgt ccacagcgcc
     1621 gtggcctggt tgaccgtttc ccattccggc gcggcgtcct tgtcctcgtc cttcccttcc
     1681 tcggcgaaac gctccttcag catcagcacc ggcagcgaga tgtggtcgga gtagcgcttc
     1741 acgattgagc gcacctgcca ggcgtcgagg aactcgcccg cctcctcctt caggtgcagc
     1801 accaccgtgg tgccgcgctc ggcgcgctcg atgtcctcga cgctgaactc gctctccccg
     1861 gccgattccc agcgcacgcc cgcggcggcc tcgagcccgg cgcggcggct gaacacctcg
     1921 acgcgatccg cgaccatgaa ggcgctgtag aagcccacgc cgaactgacc gatcagctgc
     1981 gagtccttgc gctggtcgcc cgagagctgc tgcacgaact gcgccgtgcc cgagcgcgcg
     2041 atggtgccga ggtggctgat cgcctcctcg cgcgacaggc cgatgccgtt gtcggtgatc
     2101 gccagcgtgc gcgcgtcctt gtcgatcgcg atgcggatct cgaggttcgg gtcgtcggcg
     2161 agcagcgccg ggttcgagag cgcctcgaag cgcagcttgt cggcggcgtc ggaggcgttg
     2221 gagatcagct cgcgcaggaa gatctccttc ttgctgtaga gcgagtggat catcaggtgc
     2281 agcagctggc gggcttcggt ctggaagccc atggtttcct tgtgcgcgac ggtcatgcgg
     2341 tgatctccct gccagtgaac gggtatgggc gcgatatggg gccgcgcgcc ggcttttcaa
     2401 gccgcccgga tcctgaagtg acacctgctg cggcatcggt tagagtcccg ccgcatccat
     2461 gccgcccgcg cgccccggcc gaccgagccg ggccgcaggc gttgtcccgc cgatcaggta
     2521 ttcgcatgca gggaagtaag gcatgacgac acagcagaat ccttgtcgct tggcgtccac
     2581 ccgccgcgcc gccgcgcagg caggctcgtg agcgccccgc cgcgccggcg ccccggcggg
     2641 cgcaatgccg cggtacggct cgccgtcgcg cacgccgtgc tgtcctacct ggaacagggg
     2701 cagcccgatt tcagcgtggc cgacgtggcc gcgcgcgccg gcgtgcaccg cgcgacggtc
     2761 tatcgccgct ggccgtcacg cgccgcgctg ctcggcgagg cgctgtcgct gcagtacagc
     2821 cgcgtcacgg tgccgatgac gggggatttc gcggcggacc tgcgcacgct gtcgcaccgg
     2881 ctcgcgcact acctcgccga accggtgctg cgcaccgtca acggcatggc gatcgccgac
     2941 agcgagcccg gcttcggcgc gctggtgaac acgcactggg aaccggtgaa acagcgcatg
     3001 accgagatga tcgaggccgg cattcgcagc gggcagttgc gccgcggcac cgatcccgag
     3061 gcgatcatcc gcctgctggt cggcccgctg ctgatgacgc cgctgttcac gcaggcaggc
     3121 gccacgcagg cgcagatcaa ccagaccgcg gacgccatcc tgcgggcgtt tctcgcgcgc
     3181 ccgtaggtcc gcattcatgc ggaagttgtc cggttgaaaa ccggacctac gagtaaccag
     3241 cgccggacgg ggagtaccag cggagcatct gctcgggcgc gtcgctgaaa tcgcggcggc
     3301 tgcccggcac gcgccaggcg tgctcggcct gccccgggct ataggagaag cagatgcccg
     3361 agggcatcgg gtcctcggat gcgggaccca tcgagacgct cgccatgtcg tagatgcgcg
     3421 agagcgcgac gaagtagccg cgctgcagga tgagcgcgaa ctcgagctcc tcgccggcca
     3481 gattcgcgac cgcccaactg gtgaagccgc ccagcacccg ctgcccggcg tagcgggcgg
     3541 gctcggtgat cacgccatgg cgcaacgacc acgtcagcac gcggccctgg tcgcgatcga
     3601 gccgggcctc ggtgaggccg ccgatctcgt ccgggacctc gatgcggtag acgcgttcgc
     3661 gcgcggcgcg gaacacatgg gcgatcgcca gcgcggccag gtcgaacagg tgggtgcaat
     3721 tgcgccgcgc atcggcgtgg cgcttcaact cgatgatcga tgcgctgagc ggcgcaccca
     3781 ccactgactg cagcgccgcg gtggcgccat tgcaggtggt cagcggatag cggtgcgcct
     3841 cggcggcaac ggcggtcacg cgctcgccgt catgctcgag cgtgatcgcg aaggcgtgcg
     3901 gatcgtcctc cacggccgcc tcgacgcgcg acgggccgct ctggcgcagg acgatggtgc
     3961 gacggtagca accgctgcca tagcgcggat cgagggcggc cgtgtagccg cccgcggtgt
     4021 tgctcatggc gcggcgctgc tcaccagccg gtgatcgcct tgagcgcgga accgatctcc
     4081 gccaggctgc gcacggtctt cacgccggcc gcctcgagtg cggcgaactt ctcgtccgcc
     4141 gtgcccttgc cgccggagat gatcgccccc gcatggccca tgcgcttgcc cttgggcgcg
     4201 gtgacacccg cgatgtagga cacgaccggc ttggtcacgt tggccttgat gaaggccgct
     4261 gcctcttcct cggccgagcc gccgatctcg ccgatcatca cgatcgcctc ggtcgccgga
     4321 tccttctgga acatgccgag gatgtcgatg aagttcgagc ccgggatcgg gtcgccgccg
     4381 atgcccacac aggtcgactg gccgaaaccg gcgtcggtgg tctgcttcac ggcttcgtag
     4441 gtcagcgtgc cggagcgcga cacgatgccg acacggcccg gcttgtggat gtggccgggc
     4501 atgatgccga tcttgcactc gcccggcgtg atcacgccgg ggcagttcgg cccgatcagg
     4561 cgcacgccca gctcgtcgca cttcaccttg acctcgagca tgtccagcgt gggcacgccc
     4621 tcggttatgc acacgatcag cttgatgccg ccgttggccg cctcgaggat cgagtccttg
     4681 cagaacggcg ccggcacgta gatcaccgat gcgtcggcgc cggtggcggc aaccgcgtcg
     4741 gccacggtgt cgaagaccgg gaggccgagg tgggtctggc cacccttgcc gggcgtcacg
     4801 ccgccgacca ttttcgtgcc gtactcgatc gcctgcttcg agtgcgatgt tccctgcgag
     4861 ccggtgaagc cctggcagat gaccttggtg tccttgttga cgagaatgct catcgaatta
     4921 ccccttcgct gcggcgacga cggactgagc agcctcggtc aggctctttg ccgcgatgat
     4981 gttgagtccg ctctcggcca gcattttcga accgagctcg gcattgttgc cctcgaggcg
     5041 caccaccacg ggcaccttga cgcctacttc cgacaccgcg ccgatgatgc cctcggcgat
     5101 caggtcgcaa cgcacgatgc cgccgaagat gttgaccagc accgcgcgca cgctggcgtc
     5161 ggacaggatg atcttgaagg cttccgacac acgctccttg gtcgcgccgc cgcccacgtc
     5221 gaggaagttc gccggacgac cgccgtgcag gttcacgatg tccatggtgc ccatcgcgag
     5281 accggcaccg ttgaccatgc agccgatgtc gccgtcgagc gcgacgtagt tgatttccca
     5341 ctgcgcggca tgcacctcgc gcggatcttc ctgcgaggga tcgtgcattt cctgcagccc
     5401 cttctggcgg tacatcgcgt tgctgtcgac gccgagcttg gcatcgaggc agtgcaggtt
     5461 gccggcggtg gtgatcacca gcggattgac ctcgatcagc gcgaggtcct tgtcctggaa
     5521 cagctttgcg agccccatga agatgttcac gaactgcttg atctggtcac ccttcaggcc
     5581 cagcttgaac gccagctcgc ggccctggta gggctgcgcg ccggtcagcg ggtcgatggt
     5641 ggccttgaag atcttctccg gcgtctcgtg ggcgactttc tcgatctcca cgccgccttc
     5701 ggaggaggcc atgaacacga tgcggcgcga actgcggtcg accacggcgc ccaggtacag
     5761 ttcctcggcg atgtcggtgc aggcctcgac caggatcttg ctgaccggct ggcccttctc
     5821 gtcggtctgg taggtcacca ggttgcgccc gagccagtgc tgcgcgaagt cgcggatctc
     5881 gtccttggtc ttgaccagct tcacgccgcc ggccttgccg cggccaccgg cgtgcacctg
     5941 tgccttgacg acccacatgt tacccccgat ctgttcggcg gcctcgacgg cctgcctggg
     6001 ggtttccgcg gcgtagccct tggacacggg cagaccgtac tcggcaaaca gctgtttgcc
     6061 ctgatattcg tgcagattca tggcgtgtta ccgtcctgac gtgaaaatga atggacccgg
     6121 accggtcggc ttgcgcaagt ccagcccccc gggaacgggg tcccgcggag ggggccggcg
     6181 aaagcgggcg caattatccc ggatattccg ccgcgagtca taccctattt gcggggtttg
     6241 cggttgggaa tatggatggc atgccccagc gccgccagcg ccgcctcgtg cacgccctcc
     6301 gagagggtcg gatggctgaa gacggtgagc gccaggtcct cggcgctggc gccgaattcc
     6361 atcgcgatca cggcctgctg caccaggtcg gcggcgctgg cgccgacgac gtggcagccg
     6421 aggacgcggt cggtctcggc ctcgatcagc agcttcacca tgcccgcgct gtcgttggcg
     6481 gccagcgcgc gtccgttggc ggcaaaggga aacaccccga cgttgtaggc cgtgcccgcc
     6541 gccttcagct gctgctcgtt ggccccgacc caggcgatct cggggtgcgt gtatatgacc
     6601 gaggggatgc agtcgtagtt gagctgcgtc ttgcgcccgg cgatgcgctc ggccaccatc
     6661 acgccctctt ccatcgcctt gtgcgccagc atcgggccgc gcacgatgtc gccgatcgcg
     6721 tagacgccgg gcatgtcggt ctggcagaac tcgttgacgt gcacgaagcc gcgctcctcg
     6781 agattcacgc cgctgtcggg cgcgaagagc ccctcggtga ccggtcggcg cccgaccgcc
     6841 acgatcaggc ggtcgaacac ttcctgtttc tccgtgtcgc cctcgacata gctcacgctg
     6901 accatgggct tgccgtcgcc ggtgacctgg gtggcggtca cgcgcgcgtt caggcggatg
     6961 tcgagcccct gcttgccgaa gagcttcagc gcgtccttcg cgacctgctg gtccgccgcc
     7021 atcaggaact cgggcagtgc ctcgatcacc acgacctcgg caccgaggcg cgcccacacg
     7081 ctgccgagct cgaggccgat cacgccggca ccgacgatcc cgaggcgcag cggcacctcg
     7141 gtgaactcca gcgcgcccgc cgaatcgacg atcagctcgc cgaagggcgc cgcggggatc
     7201 tcgatcggca ccgagccggt ggccaggatc acgtggctcg catcgatgat ctcgggcgcg
     7261 cccccggcgg gcgtgaactc gaccttgcgc ccggccagca gccggccgct gccgctcaac
     7321 gcggtgacgc cgttggcctt gaacagcccg gcgatgccac cggtgagctg gcccacgatc
     7381 tggcgcttgc gcttctgcat cgcctcgagg tccagcgtca gctcgccggt atggatgccg
     7441 tgcaccgcga ggccgtgccc cgcctcgtgg aacttgtgcg aactgtcgag cagtgccttc
     7501 gagggaatgc agcccacgtt gaggcaggtg ccgccgaaca ccggcttgcc gtcgccgctg
     7561 cgccacttct cgatcaccgc ggtcttcagc ccgagctgcg cgcagcggat cgccgccaca
     7621 taaccgccgg ggccggcgcc gatcaccacc acgtcgaatt gctgtgccat atcagcctgt
     7681 ctccactgct actgtttact tttaccccgc cgccgcgcag gccattgtga cgccgggaca
     7741 cgccgtgaat acatccctgt aggctcgact gcggcatcca tgccgcagac ggtccccgcg
     7801 ccacaacgcc ctgcccggcg gctgcgcaga tgcgagattc cacgcagatg ggccaatatc
     7861 attcccgagc gctgtgcggc gtcgactgag cggcctgctt gcccggcgac ctcacaaggg
     7921 gccgtggagc aggccagcga cgcgcaatcg tcccacgtta gagatccagc aggatgcgcg
     7981 ccggatcctc gatcatgtcc ttgatcgcca ccaggaactg caccgcgtcc ttgccgtcga
     8041 tcaggcggtg atcgtaggag agcgcgaggt acatcatcgg gcggaccacg acctggccgt
     8101 tcaccaccac cgcgcggtcc tggatcttgt gcatcccgag aatggcggtc tgcggcggat
     8161 tcaggatcgg ggtggacagc agcgaaccga acacgccgcc gttggtgatc gtgaaggtgc
     8221 ctccggtgat gtcctcgatc gcgagcttgc cgtcgcgcgc cttcagcccg tactcgcgga
     8281 tcttcgcctc gatgtcggcg aagctcatcg catcggcatc gcgcagcacc ggcaccacca
     8341 ggccgcgatc gctggagacc gcgacgccga tgtcctggta gccgtggtag acgacgtcgt
     8401 tgccgtcgat cgaggcgttc accaccggga agcgcttgag cgcctcgacg ctggcgcgca
     8461 cgaagaagcc catgaaaccc aggcgcgtgc cgttgtggcg cttctcgaac tgatccttgt
     8521 acttcgcgcg cagatccatc accggctgca tgtcgacctc gttgaaggtc gtgagcatcg
     8581 cggtgttgcg cgaggcatcg agcaggcgct cggcgacacg cgcgcgcagg cgcgtcatcg
     8641 gcacgcgccg ctcgactcgc ccctgctcgc tgcgcgccac cagcgccgcg gcgggcgtcg
     8701 agggcaggtc cgcgggggcg cgcgccgctg ccggcgccgc agcggatgga gtcgaagctg
     8761 ccgcgggaac cggcgcttgc ggcgcgtgcg cgagcacgtc ctcgcgggtg acgcgcccgc
     8821 ccttgccact gcccttgacc tgcgccggat ccacgccacg ctcggccgcg gccagccgcg
     8881 ccgccggacc ggcgaccccg gcctcggcgg cgggcgccgc ggcttcggct gccggcgccg
     8941 ccgcgctatc cggcgcagcg gcggtcgcgc cggcgacgaa gcgcgcgatg acttcctggc
     9001 tcagcaccgt ggcaccggta tccttgagaa tctcggcgat cgcgccgtcg gccggcgcca
     9061 ccacctcgag cacgaccttg tcggtctcga tgtcggcaac cacctcgtcg cgccggcagg
     9121 cctcgccggg gcgcttgtgc cagctcgcca ccgatccttc cgccaccgat tccggaaact
     9181 ggggtgcctt gatgtcgata ctcatggtcg gatgttcctg tcgttgctgc tgcgtgattc
     9241 aggggttggg ccagcgcggc acctgcatgc cgagcgcgtc gctgatcagc ttgtcctgct
     9301 gggcgaggtg cagcgcgctg tagccggctg ccggcgctgc cgaggcctcg cggccggcgt
     9361 attccaggta gaggtcgcgg ttgtgttcgc gcacgacctg gcgcatgtgg tgctggctgc
     9421 tgaaccaggc gccctggttc tgcggctcct cctggcacca gctcacctcg agcagctggc
     9481 ggtacggcgc gatggtcgcc agaagctcct gctgcgggaa cgggtagagc tgctcgatgc
     9541 gcacgatcgc cacgtgctgc aggtcctcgg cgttgcgccg gtcgcgcaac tcgtaataca
     9601 ccttgccgct gcagaacacc acgcgcgtga cggactcggg atcgatggca tcggtttcgc
     9661 cgatcacggt cctgaacgag ccttcggcga gctcggtgag ctcggacacc gcgtccttgt
     9721 ggcgcagcag gctcttcggg ctgaacacca cgaggggctt gcgcagcttg cgcaccgcct
     9781 gtcggcgcag caggtggtag atctgcgccg gcgtcgtcgg cacgcagatc tggatgttgt
     9841 gctcggcgca cagctgcatg aaacgctcca ggcgcgccga ggagtgctcc ggcccctgcc
     9901 cctcgtagcc gtgcggcagc agcagggtca gcccgcacag tcgcgcccac ttgtgctcgc
     9961 cgctggtgat gaactggtcg atcacgatct gcgcgccgtt ggcgaaatca ccgaactgcg
    10021 cctcccacag gatcagcgcg ttcggagtgg tggtcgcgta gccatactcg aagccgagca
    10081 ccgcgagctc gctgagcagc gaatcgtgga tgtcgaacgg cgcctgcgcc gcgccgaggt
    10141 gctgcagcgg cacgtgcacg ctgccgtcgt tctggttgtg cagcacggcg tggcggtgcg
    10201 agaacgtgcc gcggcccacg tcctggccgg tcaggcgtac caggcagcct tcctcgagca
    10261 gcgaggcgta ggccagcagc tcggcgaagc cccagttcac cggcgtggcc gccgcgctca
    10321 tcagccggcg gtcctcgtag atcttctgca cctggcgctg cagcacgaag ccctcgggca
    10381 gggcgcacat gcgcgcgccc agatccttga gcgtcgccag atccagccgc gtgtcggcgc
    10441 tggcggtggt ggcatggccg atgtagggcc gccagtcgac atagagcttg gtattgggct
    10501 cggtcaccag gctcttggcg acgtgctgcc cgctctcgag cgcatggcga taaccatcga
    10561 tcagttcctg ctcctgcgcg gcgctgatca cgccctcggc gatcaggcgc tgggcgtaca
    10621 gcgtgcgcgt ggtcgggtgg ttgcggatct tctggtacat catcggctgc gtcgcggccg
    10681 gctcgtcggc ctcgttgtgg ccacggcggc ggtagcagac caggtcgatc accacgtcct
    10741 tgcggaactg ctggcggtaa tccgccgcca gctgcgtgac gaacagcacg gcctcgggat
    10801 cgtcgccgtt cacgtggaag atcggtgcct gcaccatttt cgcgacgtcg gtgcagtact
    10861 cggtcgagcg cgcatcctcg cgccggctgg tggtgaagcc gacctggttg ttcagcacga
    10921 tgtggatcgt gccgccggtg cagaagccgc gcgtctgcga catctggaag gtttccatca
    10981 ccacgccctg cccggcgaac gcggcatcgc cgtggatgat gaccggcacg acccggtcgc
    11041 gctcgacgtt gccgcgccgg tcctggcgcg cgcgcacgct gccctcgacc accggagcga
    11101 cgatctcgag gtgcgagggg ttgaaggcga gcgcgatgtg catctccccg cccgcggtca
    11161 tcacgttcga cgagaagccc tggtggtact tcacgtcgcc cgagccctgg taggtcgcct
    11221 tgccctcgaa ctcgtcgaag agatcggccg ggttcttgcc gaagatgttc accagcaccg
    11281 cgagacggcc gcggtgcgcc atgccgacca cgagttcctt ggcgccgtag ccgcccgagc
    11341 gctgcaccag ctcgtcgagc atcgggatca ggctctcgcc gccctcgagc ccgaagcgct
    11401 tggcgccggg atagcgcgag gcgaggaact gctcgaggcc ctcggccgcg gtgaggcgct
    11461 ccagcaggtg cagcttcacg gcggcctcga atccgggtgc cgagcgcacg ccgtccatgc
    11521 gctgctggat ccagtgccgc tcctcggtgg agacgatgtg catgaactcc gcaccgaccg
    11581 tgtggcagta ggtgcgctcc agcgcgtcga cgatctcgcg cagcgtcgcc tcgtccttgc
    11641 cgatgtagag cgagccggtc tggaacacgg tgtcgaagtc ggcctccgac agctggtgga
    11701 agtgcagatc gaggtcggga acgtgctcgc gcgcggccag cccgagcggg tccaggctgg
    11761 ccttctggtg accgcgctgg cggtaggcgc tgatcagctg caccacgcgc acctgcttgc
    11821 gctcgtactc ggtactgatg cccgggtgca cgatgggagc gtccgggcgc gccttctggc
    11881 gcgcgagcag cgcaaactgc tgctgcaccg tggagtgcgg cacgtcgacc cgcgtgccat
    11941 cgacctgtgg caagcggtcg aaatagtcgc gccactgcgc cgcgatcgag ttcggatcgc
    12001 gcaggtaggc ctcgtacatc gcttccacgt aaacggcgtt gccgccggaa atgtgggagc
    12061 tgcgccacaa cagctccatc gtgccctgct gcattccgtc ctgcctccac cctggcgcga
    12121 cgcgcagtgt tgcgcgcagc cgccgcgatt ctagcccaaa gtcccgcgca tctcagccgg
    12181 gatgccaaaa agaaacggcg ccccgggggg cgccgttcgc ttgctcgcgc tgagcacttg
    12241 gcctgtatgt cgggccgaag cccgacctac ggggcgattc tgctcaggtg ccgcgttcga
    12301 gcagcatgtt gcggatatgc ccgatggcct tggtggggtt gaggctcttc ggacacaccg
    12361 acacgcagtt ctggatgccg tggcagcgga acacgctgta gggatcgtcg agctcggcga
    12421 gacgctcgtt ggtcgcggtg tcgcggctgt cgatcaggaa acggtaggcc tgcagcagcc
    12481 ccgaggggcc gatgaagcgg tccggattcc accagaacga ggggcaggcg gtcgagcagc
    12541 aggcgcacag gatgcactcg tagagcccgt cgagcttctc gcggtcctcg ggcgactgca
    12601 ggcgctcgat ggcgggcgcc ggctggtcgt tgatcaggta gggcttcacc ttctcgtact
    12661 gctggtagaa catcgtcatg tcgacgacga ggtcacgcac caccggcagc cccgggagcg
    12721 gacggatcac gaggcgctcg ttcttgaccg tctccgacag cggcgtgatg caggccagcc
    12781 cgttcttgcc gttcatgttc aggccgtcgg aaccgcacac cccttcgcgg caggagcggc
    12841 gataggtgag cgagggatcc tgcgccttca gcagctcgag cacgtcgagc accatcaggt
    12901 ccttgccccc ggtatcgacc tgcagctcct gcatcgaggg ttcgcgatcg gtttccggat
    12961 tgtagcgata gacacttact tgcagcatgt ggcaggtccc cgatcagtag gtgcgcttca
    13021 tgggctcgaa ggtttcgacg gtcttcggct tgaaattcac gccgcgcttg cccacccgct
    13081 tgtcacccgg caggtacagc gaatggcaca gccagttctc gtcgtcgcgc tccgggaagt
    13141 cctcgcgcgc gtgcgcgccg cggctctcgg tgcgggtctc tgccgcgatg gcggtcgctt
    13201 cggccacttc gagcaggttc tgcagctcga gcgcctcgaa gcgcgcggtg ttgaacgcgt
    13261 tggtcttgtc cgtgaggacc acgttctcga tgcgcggacg cagttcggcg agcgccttca
    13321 cgccgtggcg catgtattcg ccggtgcgga acacgccgaa gaaattctgc atcacggtct
    13381 gcagttcctt gcgcagcgaa gcgacgctct cgccagccgt cgagctgttc aggcggttca
    13441 ggcgcgacat cgcggcgtcg acgtcggtgt cgcccgcatc acggtagtcg atgccctcgc
    13501 gcagcgcctt ctcgatgaac aggcccgagg cgcggccgaa cacgatcagg tcgagcagcg
    13561 agttgccgcc caggcggttg gcgccatgca ccgagacgca cgccacttcg ccgcaggcga
    13621 agagtcccgg gatcacctgg tcgttgccgc cggcgtcctg cgtgagcgcc tggccgtgca
    13681 cattggtcgg gatgccaccc atcatgtagt ggcaggtcgg gacgaccggg atcggctcct
    13741 tgaccggatc gacatgcgca aaggccttcg acagttcgca gatgcccggc aggcgcgagt
    13801 tcagcacgtc ctcgccgagg tggtcgagct tcaggaagac gtggtcgccc tcggggccgc
    13861 agccacgccc ctcgatgatc tcgagcacca tcgagcgcgc cacgacatcg cggcccgcga
    13921 ggtccttggc gttcggcgcg tagcgctcca tgaagcgctc gccgttcttg ttcagcaggt
    13981 agccgccctc gccgcggcag ccctcggtga ccagcacgcc cgcgccggcg atgccggtgg
    14041 ggtggaactg ccacatctcg atgtcctgca ccgggaagcc cgcgcgcagc gccatgccca
    14101 caccgtcgcc ggtgttgatg tgcgcgttcg tggtcgaggc gtagatgcgt cccgccccgc
    14161 cggtggccag caccgtcgcg cgcgatttca cgtagacggt ctcgccggtc tccatgcaga
    14221 tcgcgatcac gccgaccacc gctccgtcct ggttcttcac caggtccacg gcgaaccact
    14281 cgttcaggaa ggcggtcttg ctcttgaggt tggcctggaa cagcgtgtgc agcagcgcgt
    14341 gaccggtgcg gtcggccgcc gcgcaggtac gcgccgcctg gccgccgcgg ccgtagtcct
    14401 tgctctgacc gccgaagggg cgctggtaga tgcggccttc ctcggtgcgc gagaacggca
    14461 gcccgaggtg ctccatctcg aacaccgcct gcgggccgac cgagcacatg tactcgatcg
    14521 catcctggtc gccgatgtag tcggagccct tgacggtgtc gtacatgtgc cagcgccagt
    14581 cgtcgttcgg gtcggcgctg gcgatcgcgc aggtgatgcc gccctgcgcc gacaccgtgt
    14641 gcgagcgcgt cgggaacacc ttggaaatca ccgcggtgcg gtagcccgac tgggccagct
    14701 gcagcgccgc gcgcatgccg gcgccgccgc cgcccacgat cacgccatcg aaactcagag
    14761 tccggattct cgccattgct caattacccc acaaaatctg aacgccccag acgaggtagg
    14821 cgaacagcac caccgcgtag gccatctgga acacgatcct gatgccgttc gccttcgcgc
    14881 ccagcatgcg caccgtcagg taatcggtgc tcaccgacca caggccgatc caggcgtgca
    14941 ccacgatcga cagcagcgcc atcaggctga acaccttcat cgcggtgcca tcgaagtgct
    15001 cgcgccacgt ggcgtagtcg atacccggcg tgaacagcag gatgccgccc acgcacagca
    15061 cgtaggcgag caggaccacc gcggtgaaac gctgcagcag ccagtccgac agaccgctgc
    15121 gaccgaaact cgtgactgcc cttaccatac ccacaccccc gcgagaacga tgagtaccgc
    15181 cgccaccgcg aaggtgatgc gggccgccgt gatgcccccc tgcagggttt cgccgatgcc
    15241 gagatccatc agcaggtgct ttacgccggc gacgcagtgg tagatcagcg ccgcgagcac
    15301 gccccagatc accagtttgg ccggcgtcgt cgcgagcagc tcgcccgcgc gcgcgaagcc
    15361 agcctcgtcg gcgagcgaga ggtcgaacag gtagagcagc acggccacgg cgacaacaag
    15421 cgccacaccg gagacgcggt gcagtatcga ggccttcgcg ggcaacggca gggcaatcgt
    15481 gcccaggtcc aggttgacgg gtctgtttgg cttcacggct ctgatacctt ccttgagatt
    15541 tcccgattga ggtaacgcat ggcaggcgcc cgggcatcgc acaccggcgg agcggtagcg
    15601 cacgaggcgc ggaccggagt cggggcgcgc cggcgggcgc gttcgaaacg cggcggatta
    15661 tagggacatg cccccctgat tacaaaccgg caacgggacc gcgtgaagca ccgcgcgcac
    15721 gcgccccgca gcgggaccag ggcggagtgt cgaattgaca atgcaacgga cccgtctata
    15781 gagttagccg ccgcgccgca tcgcggcctg ccccccgaaa cgttgcaagg agtcgtagtt
    15841 acatgacggg aaaaaccgcc agattgacga tacagggcgc cgcggcggac ctcgaattgc
    15901 ccgtttattc gggctccatg ggacccgacg tcatcgacgt gcagggactc accccgaagg
    15961 gttacttcac ctacgatccg ggattcgtat ccaccgcggc ctgcgagagc aagatcacgt
    16021 tcatcgatgg cgacaagggc gtcctgctgc accgcggtta cccgatcgag cagctcgcgg
    16081 acaagtcgga ttacctcgag acctgctacc tgctgcttta cggagaactg ccgagcgcgg
    16141 cgcagaagca gcgcttcgtc aacgtggtga tgcgccacac gatggtgcac gagcaactgc
    16201 agttcttcta ccgcggcttt cgccgcgacg cgcacccgat ggcgatcatg tgcggcgtgg
    16261 tcggcgcgct gtcggccttc taccacgacg cgctgaacat caccgacgag gagcaccgcg
    16321 agatcgccgc gttccgcctg atcgcgaaga tgccgacgat agccgcgatg agctacaagt
    16381 actcgatcgg catgccgttc atgtacccgg acaacacgct cgactactcg gcgaacttcc
    16441 tgcacatgat gttcggcaat ccctgcgaga aatccgtgat caatccggtg ctcgcgcgcg
    16501 cgatggaccg catcttcctg ctgcacgccg accacgagca gaacgcctcg acctcggcgg
    16561 tgcgcctgac cggctcgacc ggcgccaatc ccttcgcctg catcgcggcc ggtatcgccg
    16621 cgctgtgggg cccggcacac ggcggcgcca acgaggcggt gctgacgatg ctcgagcaga
    16681 tcggcgacga gtcgcgcatc ccggagttca tcgcgaaggc caaggacaag aacgacccgt
    16741 tccgcctgat gggtttcggc caccgcgtct acaagaattt cgacccgcgc gccagggtca
    16801 tgaaacagac ctgcgacgag gtgctggccg agctcgggct cgagaaggac ccgctgttcc
    16861 gcatcgcgca gaaactcgag cagatcgcca ccgaggacga gtacttcgtc gagaagaagc
    16921 tctaccccaa cgtggacttc tactcgggca tcatcctgcg cgcgctcggc atcccgacca
    16981 acatgttcac cgtgatcttc gccaccgggc gcaccgtggg ctggatcgcg cactggcacg
    17041 agatgatcag cgcgagctac aagatcggcc gcccgcgcca gctctacacg ggctacacgc
    17101 cgcgcgacta cgtcccgatg gaccggcgcg gctgagcacg atacccggcg gcgcaacgcg
    17161 ccgccggcca ttcacgcaca cgccgcctgc accgggcgcc gagcgtcccg ggggcgcggc
    17221 aggagttccg gcatgtccgc tctgcatgtt cctccccgcc gcgccgcgcc ccggtcccgt
    17281 gctgcgcact ccacctcgct gctgaatgct ctgtccgaga tgcgcgccct cgccgagata
    17341 ggcagctatt tcggcattcg cccgctgctg gatcgcgtgg ttccgcgcgg cgacggacac
    17401 ccggtgttgg tgctgcccgg gttcatggcc tccgacacgc tgacgcaggg actgcgggac
    17461 ttcctgcaac ggctgggcta cgaggtgcac gcctgggagc tcggtcgcaa cccgggcctg
    17521 cgcctcgatc tgtgcgagaa gctcgaggct cgcatcgagg cgatccgcgg tgcctcgcac
    17581 cggcgcgtga gcctgctcgg ctggagcctc gggggcatgt acgcgcgcgt gatcgcgcag
    17641 cggcgcagcg atgacatccg ccaggtgatc accctgggca cgccgttccg ggtgagcgcg
    17701 gaggacggcg gcaagggcgc catcgcccgg atgtacatga agctcaatcc cgatgcgtcc
    17761 gcggaccccc tgatggccag cgggctcgcg cgcctgccga cgccggtgcc ctcgacctcg
    17821 atctacagca cgcgcgacgg catcgtgccg tgggcgagct gcgtcgagca atgcgacgcg
    17881 cgccacgaga acatccgcgt gcacggcagt cactgcggca tgacgtacaa cccgctgatc
    17941 ctcgcgatcg tcgccgagcg cctagggcag cgcgaacacg cctggcgccc gtttcgtccc
    18001 tcgggcctga aagccatcct gtcgcggccg gtctgcgacc ccgtcacggc ctgagagccg
    18061 cgtcgcggcg cgcgagcgcg gccggcaggc gccctacttc cagctcttca cggtcgactc
    18121 gatgttctcc aggcccacgt ggcgcaggtc ctggcccttg acgagataga acacgtattc
    18181 ggcgatgttg cgcgagtggt caccgatgcg ctccagcgag cgcagcgccc acatgatgtt
    18241 catcacgcgc gagatcgagc gcggatcctc catcatgtag gtgaccagct cgcgggtggc
    18301 cgagcggtac tcctggtcaa ccgtcttgtc gcgacgcacc acctccagcg ccgcatccac
    18361 atcgagtcgg gtgaaggcat ccagcgcgtc gtgcagcatc tcggacacgg cggcgccgat
    18421 gtggcgcaac tcgaagtagc cgcgcggcgc cggcccctcc tccgtgagct tgagcgcgag
    18481 cttggcgatc ttcttcgcct cgtcgccgat gcgctccagg tcgcgcaccg ccttggtgat
    18541 gatcagcacc aggcgaagat ccgaggccgc cggctggcgt ttcaccagga tcttcgtggc
    18601 ttcgtcatcg accgcgacct cgagaccgtt gacctgcttc tcgcgcgcaa cgacctcgtc
    18661 ggcgcgctgg ctgtcggcgt cgatgatcgc gatcaccgcg tcctcgatct gtcgctcgac
    18721 gatgccgccc atcgcgagca cgcggctctt gatggcttcc agctcggcgt cgaactggtg
    18781 cgagatgtga tgctcggatg ccattgtctt tttctcccga tcagccgaaa cggccggtga
    18841 tgtagtcttc ggtcagcttg tgctgcggcg tggtgaacat ctggtcggtg ctgccgatct
    18901 cgaccagccg cccgaggtgg aaatacgcgg tgcgctgcga cacccgcgcc gcctgctgca
    18961 tcgagtgcgt cacgatggcg atcgtgaagt tctcgcgcaa ctcggccatg agttcctcga
    19021 tgcgcgcggt ggcgatcggg tcgagcgccg agcacggctc gtccatcagg atcacctcgg
    19081 ggtcgatcgc gatggtgcgc gcgatgcaca atcgctgctg ctgcccgccc gagagcccgg
    19141 tgccgggctt gtcgaggcgc tccttgactt cctcccagag tccggcgcga cgcaggctct
    19201 tctcgacgat ctcgtcgagt tcgctgcgcc gcgaagcaag gccgtggatc ttcgggccgt
    19261 aggcgacgtt ctcgtagatc gacttcggga acgggttggg cttctggaac accattccga
    19321 cgcgcgcgcg caactgcacc acgtcgagcg cgggatcgta gatgtcctcg ccgtcgagct
    19381 gcagcgtgcc gctgacgcgg cagccctcga tggtgtcgtt catgcggttc aggcagcgca
    19441 ggaacgtcga tttgccgcag cccgagggac cgatcatcgc gatgatctgg ttgggcgcga
    19501 tatcgaggct gacgcggtgt atcgcctggt tctcgccata gaagacctcg acgtcgcgca
    19561 ggcgcatctt gggcgcctcg acgaaggcct cgcccacggt ctcgccggct gcgggcatcg
    19621 cggatgtcat cgtgttctgg accatgctct cgttcccggt ggattcttgc cggcgccgcg
    19681 gctaccagcg cttctcgaag cggttgcgca ggaataccgc cagcgcgttc atgatcacca
    19741 ggaaacccag cagcaccatg atcgcggcgg aggtcagcgc ggtgaaaccg cgctcggggc
    19801 tgtcggccca caggtagatc tgcaccggca gcaccgtggc cgagtcggtg aggctgcgcg
    19861 gcacgtcgac gatgaacgcg accatgccga tcatcaacag cggcgcggtc tcgcccagcg
    19921 cgcgcgccat gccgatgatc gccccggtca gcatgcccgg cagcgcgttg ggcagcacgt
    19981 ggtgcagcac catctgcacg cgcgaggcgc cgagcccgag cgcggcctcg cgcagcgagg
    20041 gcggcacggc gcggatcgcc gcgcggctgg cgatgatgac cgtggggaag gtcaccagcg
    20101 tcagcaccag cccgccgacc agcggcgccg agcgcggcat gccgaacagg ctgatgaaca
    20161 ccgcgaggcc cagcaggccg aagatgatcg agggcacggc cgcgaggttg ttgatgttga
    20221 cctcgatcag gtcggtccag cggttgcgcg cggcgaactc ctcgaggtag atcgccgccg
    20281 ccacgccgag cgggaatgac agcagcagcg tcaccgccag cgtgtacagc gagcccatca
    20341 cggcgccccg gatgccggcg agctcgggat cgcggctgtc gccgttgccg agcaggttgc
    20401 gattgagcct cagatcgagc tgtcccgcgc cgtcgagctg gccgagccac gcagcctgct
    20461 gcgcgctgat gccggtggcc ggcggatcgc cgatgcgccc ggtctccttc acataggcat
    20521 cgaccttgcc atgggccacc agccacagcg tttccgtgcg cccgagcagc gccggctgcg
    20581 ccatcaggcg atcgcgcaac tcgtagcccg ccccggcgct cagcaggcgg aacagctcgc
    20641 gcttgtgcgc gcgggccgtc acctcgggga agcgcgcgag cagggtctgc ttcagcacac
    20701 cctcgtagtc ggccgcggcg agcgcttcgt cgctggcgcc gggcgcgatg cccagcacct
    20761 cggggtcgta atgcacctcg agctgcagct gcgtctgctc gaaagcctgc cagcccttga
    20821 gcacgatatc cgtgaacagc agcgccaggc tcgcgagcgc gaccagcacc gaggcgatgc
    20881 cgtagaggcg aaagcgacgc tcgcgccggt agcggcgctt caggccgctg cgcacgatgt
    20941 cgatggtgtt gcgccggggc gcgggactgc cgcctgggct actcatactg ctccctgtac
    21001 ttgcgcacga tgtgcagcgc gaagatgttc agcgccagcg tgaccacgaa cagcaccagg
    21061 cccagggcaa acgccgcgag cgtcttcggg ctgtcgaatt cctggtcgcc gaccagcagc
    21121 gtcacgatct gcaccgtcac cgtggtgacc gactcgaagg gattagccga caggcgcgcc
    21181 gagagcccgg ccgccatcac cacgatcatg gtctcgccga tggcgcgcga agcggccaac
    21241 agcaccccgc cgacgatgcc gggcagcgcc gcggggatca ccacgcgctt gatggtctcg
    21301 gagcgcgtgg caccgagcgc cagcgagccg tcgcgcaggc tcagcggcac cgcgtgcagg
    21361 atgtcgtccg acaacgagga cacgaaggga atgatcatca cgcccatcac caggccggcg
    21421 gccagggcgc tctcggaaga ggcgtcgagc ccgatcgcgg cgccgaaatc gcgcaccagt
    21481 ggcgccacgg tgagcgcggc gaagaaaccg tagaccacgg tcgggatgcc ggcgaggatc
    21541 tccagcagcg gcttgagccg gctgcgcagc ttggcgctgg cgtactcgct gaggaagatc
    21601 gccgacatca acccgaccgg caccgccacc agcagggcga tcgccgagat cagcagcgtg
    21661 ccggtgaaca gcggcaccgc gccgaagcgc cccgaggagc cctgctggtc ggcacgcagg
    21721 gcgatctgcg ggctccactc cagcccgaag aagaactcca ccggcgagac catctggaag
    21781 aaacgcagcg actcgaccac caccgagagc aggatgccga tcgtggtgag cacggccacg
    21841 caggcgcagg cgaacagcag gcgcgtgaag atgcgctcga ccacgttgcg cgccacgaaa
    21901 tcggcccgca cccggtgcca gccgcggaaa ccgccaagca gcgcgcaggc cacggcctgc
    21961 gtccacatgc ccatcgacga gcgttgcccg aggctgacca tgcgttcggc ggcgctcacg
    22021 agcacgggat cggtggcgcc ggtggcgccg cccgcggcca ggttggcgat ggaattcagc
    22081 agcagtccgg cgtcggttcc ggcatgcgag gccggcaacg actccagcac cagcctctcg
    22141 atgagtggcg cggaggccag cgaccacacg agcaccgcgg cgagcgcggg caacagcacc
    22201 atcaatgcga cgtaggcgcc gtaatgcgca ggcagggacg gcaggcgacg cacgcccccg
    22261 agcggtaccg cgaccgacac ggcgcgcgaa ctgccctggt aatagcccgc gacggcaagg
    22321 gcggcgatca gcagcaacag catggaaaca ttcatggcaa agcgctcacg tcatcctggt
    22381 ccggacccgg ccaccgcggg gatgcccgcg gcggccgggt ccgaatgggc tccccggctc
    22441 agagcgcgaa cggcgccagg ctgcgcgcgg ccgcggccgt cgcctcgtac tggtcctgcg
    22501 gcagcgcgat cagtccacgg tcggcgaggt agccatcttc gcccatggcg cgttcgctga
    22561 caaactccgc gacgaactcc cggatgccgg gaatcaggtc cgcgtgcgcc gtcttcacgt
    22621 agaagtagag cgggcgcgac acggcatagg agccgtcggc gatgctctcg tacgtgggct
    22681 caacgccggc gaccttgctg ccctgcacct tgtcggcatt ctcctccatg aagctgtagc
    22741 cgaagatgcc gagcgaattg gggttcgcgc cgagcttctg cacgatcagg ttgtcgttct
    22801 cgccggcctc gatgtaggcg ccgtcctctc gcaccgcgtg gcagaccgcc ttgaagcggt
    22861 tctcgtcctg gtccttgatc gccttgaccc aggggaagga cttgcagccg ccttccatcg
    22921 ccagctcgac gaaggcatcg cgggtgccgg aggtcggcgg cgggcccagc acctcgatcc
    22981 ggttggccgg cagagcgggg ttcacgtcct tccaggtctt gtaggggttg ggcaccagcg
    23041 tctcgctgcc gtcggggttc ggcacatcct tggcgagcgc gaggaacatg tccttcagcg
    23101 tcagctcgta atgatgcgcg ctgcgtgcat tggcgatcac gatgccgtcg aaaccgatca
    23161 cgacctcggt gatcccggtc acgccgttct tggcgcaatc cgcgaattcg gaatccttca
    23221 tgcgccgcga ggcattggcg atatcggggt gctgcacgcc cacaccggag cagaacagct
    23281 tgatgccgcc gccggtaccg gtggcctcga ctttcggcgt cttgaaggtg ccggcgcgac
    23341 cgaagcgctc ggccaccacg gtcgagaacg gatacaccgt ggacgaaccc acgatgctga
    23401 tgttgtcgcg ctgggcgagc gcgaccggga cctgggtcag cgcggcaacg gcgatggcgg
    23461 tgctgagcag tttcttcacg ggagcctcct gggctgttgc tggactcgat tcagcgagac
    23521 tggatcagat gcgcgtcgat attatgaatg ttttatgact gtttcgtgac agcgcgaatc
    23581 agccctgcgc cgtgccgctc agccgtgcca gcgggaagcg gcaggtgaag gtgctgccgg
    23641 cgcccgggcg actctcgatc tgcagttccc cttcgacgcg cttgagcgcg tgcttgacga
    23701 tcgcgagccc cagccccgtg ccgccgctct ccgccgaacg gctcttgtcg acgcggtaaa
    23761 agcgctcggt gaggcggggg atgtgcacgg ggtctatgcc gatgccgtcg tcgctgacgc
    23821 gaaacagcgc gtgcccgccg ccggcctcga acacgacgcg gatctttccc cccgcgccgg
    23881 tgtacttcac cgcgttgaac accagattcg cgaagatgct ctcgatctgc agccgcggtc
    23941 cgcgcaacct caggcccggc gcgcactcga gcgtgatctg gcgctcgccg ccgcaggcgg
    24001 ccagcgcgtt ctcgcggatc gaatcgagca tcgcgcagac cgcgatcgac tcctcggcgg
    24061 aggaatcggc actttccgtg ccctcgagcg tcgacagcaa catcaggtcg cgcagcaggt
    24121 gctccatgcg cgccgactgc tgctgcatct gccccagcac cttggcgatc accggcgagt
    24181 cctgcggcag gaggtcctcc agcgttccga cgtagccggc gatcaccgtg agcggggtgc
    24241 gcagctcgtg cgatatgttc gcgacgaaat cgcggcgcat cgtctcgagg cggttcagcg
    24301 ccgtgacatc gcggatgaag atcaccttgc gattggcgcc aaaggaggtc gcctgcacct
    24361 ccagggtgcg cgccgccgcg ccaccgatct cgagctcgaa ggaatgctcg aaatcgctgg
    24421 ccgcgagata ggccgtgaaa tcgggatcgc gaaccaggtt ccccagcggc tgccccgcgt
    24481 cctgcgggta gcgcagcccc agcagcgtga gcgcgctggc gttggaccac tcgatggtgt
    24541 tgtccggacc cagcatcacg atgccgtcct tcagggcggc gtaggattcg cgcaggcgca
    24601 gcactgccgc atcggcctcc tcgagttgtt tttgcaggcg ggcatgggtg tgctgcatgc
    24661 gatcggccac gtcaccgagc atgccgccct gcaccggcgg atcgcgctcg agcgggccgt
    24721 ccgcgagcca gcgctgcagt ttcgccatgc ggcggtagct gatcagcagg tagaccagca
    24781 ccccgcaggc gagaccggcg ccggcgttgc ccgacacatc gcccagcagc agccccgcga
    24841 gcatcagcgc ggcgagcagc agcaggtcgc gccccgggct ggtcggcatg ctcaggcacg
    24901 cgacatctgg ttggagaagc ggtaaccggt gccgcgcacg gtctggatga actccccgaa
    24961 atccttcgcc ccggtcactg ggggctccag cgccttgcgc aggcgccgca cgtgcacatc
    25021 caccgtgcgc tcgtcgatgt agacgttcgc gccccagacc tggtcgagca gttgcgcgcg
    25081 cgagtaggcg cggtccggat ggctcatcag gaactgcagc aggcggaact cggtggggcc
    25141 catctcgacc gggcgcgcgt cggcgaacac gcggtgcgcc agcgggtcga ggcgcaagcc
    25201 gtcgaactcc atcggctgct gctcctcgac cggcgcgctg cgccgcagca gcgcgcggat
    25261 gcgcgcgatc agctcgcgcg gcgagaaggg cttgctgacg tagtcgtcgg cgccgccgtc
    25321 gagcccctgc acgcgattgc cctcctcgac cttcgccgtc agcatgatga ccggcaggcg
    25381 cgcgcagttc tcgtccttgc gcaggcgccg cagcagttca tagccgctgg tgtcgggcat
    25441 catccagtcg agcagcacga ggtcggggcg ggcgtcgagg atctgcccca tcgcctcgcg
    25501 ggcgttggcc gcctgcaggc aattgaagcc ggccagctcc agcgagagct gcaccatgtc
    25561 ccggatcgag gactcgtcgt ccaccaccag gatcgtctgc ttgccgttca tcctgttccg
    25621 tcgcctcgcg tcgtcatgat cgttgcgtga cagtaaaaga cagaattatg acgtttcggt
    25681 gactcgcgcc agccggtggc ggatacgata cagccggtgc ggcacgggct tgcggttgaa
    25741 gtccggatcg atgctctgct ccgtgaggtc actgatctcg agatcggcca gcgccccggc
    25801 gtcgaggcga aagccgcgcc ggttggtgga gaacagcaag gtgccctcgc gctccagcac
    25861 gcgcagcgcg gcgcggatca gcgcgacgtg gtcgcgctgc acgtccagcg tctcctccat
    25921 gcgcttggaa ttggagaagg agggcgggtc gaggaagacg agatcccagc gccggcgctc
    25981 ggcacccagc cagcgcaggc agtcggcgcg caccagttcg tgctgcgcct cgggcagccc
    26041 gttcagcgcc aggttgcgcc gggaccagtc gaggtaggtc gccgacatgt cgacgctggt
    26101 ggtggcgcgc gcgccgccga ggcccgcgaa cacgctcacc gcggcggtgt agcagaagag
    26161 gttcaggaaa cgcttgccgc gcgcctcggc cgcgaccatg cgccgcagcg ggcggtggtc
    26221 gaggaacagg ccggtatcga ggaaatcgcg caggttcacc agcaggcgcg ccggcccctc
    26281 cgcgacttcc atgaagtggt tctcgcgcgc caggcgctgg tactgttgcg tgccgcgctg
    26341 gcggctgcgc gtcttcagca cgatgcgtcc cgggggcagc tgcagcacgc tggcgagccc
    26401 ggcgcgcact tcctcgaggc gctcgcgcgc gagcgcctcg tcgacccatg ccggcggcgc
    26461 gtactccgcc acgtgcgccc aggcgccgta gcgatccacc gcgaccgcgt attccgggat
    26521 gtcggcgtcg tagatgcgat agcactgcgg ctgctgggtg gcgacccagc ccttgatgcg
    26581 gcgcaggttc ttgcgcacgc ggttggcgaa catttctgcg ccggcgctca acgggccggc
    26641 gggcaccgcc tcggtctgct gcgccggctc cccggcttcg gcgtcgcccg ccgcctcggc
    26701 ccggcgcgtg gcgcgcgtgc tcagcgcggg gtcgtgcacc agcaggcggc agtcgatggc
    26761 gccgttgcga aagcggtagc gccgcgcgaa gcgcagcccc agcgcggcct ccagcgggcc
    26821 ctcggtggtg aggatcgcgg cgcgccagcc gggaaactcc tcgcgcacca gccggccgag
    26881 atcctcgtac agcccctgca gctcgcgcac ctcgcccagg cgctcgccgt aaggcgggtt
    26941 gcagatcagc agccccgtgc gcccggccat cgcctcgggc cggcgttgcg cggcgagcgc
    27001 gaccgccgaa aactcgatgc ggcccgcgag acccgcggcc gcagcgttgt cgagcgcgtg
    27061 cgccagcacg cggcggtcgg catccgcgcc ggcgatcggc ggcaatccgg gcagcccctg
    27121 ctcgcgcgcc gcgcgcgcct cctgcaccag gcgctgccac aacagcgggt cgtggcccgg
    27181 ccacttctgc cgcccgagat cgcgccactc cccgggcgcg gcaccggcgg cgatcagcgc
    27241 gccctcgatc agcagcgtgc cggaaccgca catcgggtcg tacagcgcgc cgcccgcggc
    27301 ggccagctcg ggccagccgc agcgcagcag cagcgccgcg ccgaggttct ccttcagcgg
    27361 tgcggcgccg gtggcgccgc gatagccgcg acggtgctgc gcgccgccga gatcgatcgc
    27421 gagcgtgagg cgctcgcggt tcagccgcgc cacgatacgt atgtccggcg cgcgcacgtc
    27481 gacgtcgggc cgcaggccgg taaccgcgcg cagccggtcc accaccgcgt ccttcacgcg
    27541 ccgcgcgttg aacaggctgt tgcgcaactg ctccgagacg ccgatgaaat ccaccgcgat
    27601 cgtcgcgccc gcgccgatgt gctgctccca cggcagttcc accgcgttgg catacaactc
    27661 atcggcatcg cgcgcgccga cctcgccgac cgcgaccagc acgcggctgg cgacgcgcga
    27721 ccacaggcag gcgcggtagg cgtgcgccag cggcccggtg atgtacacgc cgcccgcacc
    27781 gacccgcgtg tcgtcgacac cggccgcctt cagctcggcg gcgagcagct cctcgaggcc
    27841 gcgcgagcag ctcgcgaaaa aatggccgtg gcgctgcgcg gggcgcgatc cgatgcgcgg
    27901 cttcgccgat tccatgttcg ccctgccctc agcctgcgcg cccgcgcgcg atcgccgcgg
    27961 ccgccgtgcc gatcagcgcg ggaccaccgt agatgaagcc ggtatagagc tgcaccagcg
    28021 aggcgcccgc gcgcacgcgc ccggcggcgt cctcccccgt catgatgccg ccggcgccga
    28081 tcagcggcag ctcgccggcc agtcgatcgg caagccgtgt catgaccgcg ttggcctggc
    28141 gcgtcagcgg cgcgccgctc agcccgccgc tctccgtggc gttcggcagg ccctcgacgc
    28201 cctggcgcga caccgtggtg ttggtggcga tcagcgcgtc catgcgcgcg cgccgcgcgc
    28261 tgtcggcgac gcggtcgatg tcctcggggg ccagatccgg cgcgattttc agcaccagcg
    28321 gcacgcgccg cccgtgctgc tcggcgagcg cctcgcgccg cgccaccagt tgcgccagca
    28381 gctgctccag cggctcgccg aactgcaggt cgcgcagccc cggcgtgttc ggcgaggaca
    28441 ggttgatcgt gatataggac gccagctcgt acacgcgctc caggcacaac aggtagtccc
    28501 cggccgcgcg ctccagcggc gtgtccttgt tcttgccgat gttgatgccg agcacgccct
    28561 ggtactcggc ctcgcgcacg cgcgcgcaga ggtagtcgac gccgaggttg ttgaagccca
    28621 tgcggttgat gatcgcccgt gcctgcggga tgcggaacag ccgcggctgc gggttgcccg
    28681 gttgcgggcg cggcgtgacg gtgccgatct cgaggaaacc gaagccgagc gcggccagcg
    28741 cgtcgatatg gtcgccgttc ttgtcgaggc ccgcggccag ccccaccggg ttcgggaact
    28801 cgatccccat gcagcgcacc gggtccggcg ccgggcggct cgcgatcagt gcatccaggt
    28861 gcagcccgtg cagcgcgtcg agcgcgcgca acgacaccgt gtgcgagcgt tcgccaggca
    28921 gcaggaacag cagtcggcgc agcagggagt acggcagttt cacgcggctg atggaccggg
    28981 ttgttcgggg gcgcacagga taaccgatcg gccgctgatc cggatcgcag ctgcggcttg
    29041 cgcccgaatt tgtgccgctc gcagatcagg cgagagtccg gcgcaggtcc ggcttcagcc
    29101 ggacattgcc ccggtaggtt cggcttcaga gtgtgtcaca aaagccatca ggttgcggcc
    29161 gagcggcgtg gtgcgtcgct ggctgtgctc cccgccattg tcatgcgggg cacgcacgag
    29221 tacgtccctg tagctccgcg ccgcgtcctg cggcgcaa
//
LOCUS       DAORUQ010000019        29214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1151_length_29214_cov_6.381186, whole genome shotgun sequence.
ACCESSION   DAORUQ010000019 DAORUQ010000000
VERSION     DAORUQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29214
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_29214_cov_6.381186"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLW13_03235"
     CDS             complement(<1..273)
                     /locus_tag="PLW13_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HQY69412.1"
                     /translation="MQSASVLRDFLLVNPVFTLFLALGCGYLVGRLRIASISLGPVAG
                     ALLVSLLLGQFGFKISEGAQSVGFALFIFAVGYQAGPRFFEVIRTQG"
     gene            complement(325..1725)
                     /locus_tag="PLW13_03240"
     CDS             complement(325..1725)
                     /locus_tag="PLW13_03240"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HQY69413.1"
                     /translation="MNTPYIRRAARPIFPRLLLAVAVSCASLARADDISELRQELEAS
                     RVAIERLEQRIRQLESAQAAPAGLSSETVPPAGSATAVVAAPAPASSPAARSAADAKL
                     DIYGFIQLDTIYDVNQSDPDWTDTLRVSKIPVNCPGDAGCGKDGETIFSVRQTRIGIR
                     GTTPTALGELNTRLEFELFGVGVDAGQTTPRLRHAWAELGDFGAGQTWSLFMDPDVFP
                     NTIDYWGPTGMMFLRNPQLRWTAYRGKGSQFAIAIESPSSALDTGKVQDIDPEDNPLS
                     IRSENNLPDLTAQWRASGDWGHAQLAGILRSVGYETESSRNGDPSGDELGWGLNASTS
                     IVFGARDKLHLQLAYGEGIASYINDGGNDLAPNGDLDEAEVLPLLGALVYYDHYWSEN
                     WSSSIGWSVAEQDNSDGQFGSAYQGARYGNLNLLYYPVPGVMVGGELVYGELELRDGS
                     EADDTRLQMSFKYNFD"
     gene            complement(1847..2869)
                     /gene="glsA"
                     /locus_tag="PLW13_03245"
     CDS             complement(1847..2869)
                     /gene="glsA"
                     /locus_tag="PLW13_03245"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HQY69414.1"
                     /translation="MTARFSVTIIAALAVFVLGVTGASAAELSPRAINAALDAAYEKY
                     RGLQEGKNADYIPALAKVDPELYGIALVTADGKVYTKGDVSSEVSIQSISKVFTMAKV
                     IEEKGPDAISGGMGVDATGQVFNSIVAIEQHKGAEMNAMVNPGAIAATSMVAGASREE
                     IWNKILAYYSDFAGRKLSVNQEVFESEAATNQRNQAIGRLMYAYGHIKENPDQATDIY
                     TEQCSVNVNAKDLATMAATLANGGRNPVTGKQVLNSAYVPNVLAVMATAGLYDDSGQW
                     LYRTGLPAKSGVGGGIIAVSPGRFGIAVISPPLDAAGNSVRAQKAIADISKALGGNPY
                     TVTVRK"
     gene            complement(3072..4457)
                     /locus_tag="PLW13_03250"
     CDS             complement(3072..4457)
                     /locus_tag="PLW13_03250"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY69415.1"
                     /translation="MATFMQAIGAIAARVSLGLMCAASHALVTQEEYERLGTTLTPWG
                     AEITGEADGSYPAWTGGTRAPPGFDAASGRWPDPYPEDKPLISIDASNMAQYQDRLME
                     GQMELMRRYPGYRLDIYPTRRGFWLPERIRQNSLANARNPECKTSANGVGVYGCWGGT
                     PFPIPKTGYEAMWNHLLFYPAPGELTADSYLVNANGGISLLVINHAIGDQPYYNPEQE
                     PYQGAGLYYLRGLATNILPVREAGSQTLVWFALHFDVDDQRAWSYQAGQRRVRMAPEF
                     SYDTPVSHIGGALFYDEVQLFAGRMDRFDFQLKGRKLMYMPYNSYRMHMLRGDPAQLL
                     GPQHVKPEQARVELRRVWVVEATPLPGARHMASRKCFYIDEDSWVALAYEGWDQSGKL
                     YRVVLGNGLPDYAHGGYFMAESVQAYDLVRGQYAVMQFHLCDRCYDRRGMPYLPDAQM
                     TPARLGGRGIR"
     gene            complement(4547..5929)
                     /locus_tag="PLW13_03255"
     CDS             complement(4547..5929)
                     /locus_tag="PLW13_03255"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY69416.1"
                     /translation="MNTKKIGACLTGIALGLICAATQAALTDEEFELLGTTLTPWGAE
                     KAGSPDGSYPAWTGDLKAPPGFDPQSGLWPDPWPDETPLFSIDATNMEQHKDRLMEGQ
                     MELMRRYPSFRMDIYPTHRGVWMPPRFVEGTLKNARNPECKTSPDGVGMYGCWGGTPF
                     PIPRNGREAMWNHALRSSTTSDYVTTGYLVNANGAITLLVQTRTYNEFPYNNPDVTPY
                     EGAGKYYQRVHNIATLPARDAGTQTMLWWPLEFDKDDQRAWSYTPGQRRVRLAPEFAY
                     DTPSAQMGGTMFFDEISLFQGRMDRFEFELKGRQLMYLPYHNYRMQLLRGDPAQLLGP
                     HHFKPEMVRNELRRVWVVEATPLPGVRHMASRKRFYLDEDTWAVIAYEGWDHSNKLFR
                     VMFSNGAINYAHGGFLSYASSMQSYDVSRGQYTALQTQLADGSYVRNEVPYWPEVELS
                     PASMTQRGIR"
     gene            6261..7928
                     /locus_tag="PLW13_03260"
     CDS             6261..7928
                     /locus_tag="PLW13_03260"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HQY69417.1"
                     /translation="MHFSRLSVRALRRGALAIGIAAAGISGGASAFSVDTANPDLEIT
                     WDNTIRLNAGWRVEDIDDALGDNFTYDESDYRFEQGDMIAQRADLYSELDIVWRGNYG
                     ARVSAAGWYDHAYRDDEVEQNPLLADLPSSYRNKEYTGLTEDFYRGPYGEVLDAFVFA
                     NQTIGEVPVSLKAGQLTTYWGMAVFYAGGIAQSQHPIDGRKGVANPGSEVKELFLPLS
                     QINLQAQLTPTIAAEVQYYLDWANTRAAEGGTFLAAADLALQGPQQLGGAGAANLQRR
                     APLQPDDQHGNWGVNFKFNPDFLRGQTLGVYYREFDEKVPWLFLTLPPAEFGYRAVYA
                     ENTKLAGISFDGSLGQWAVGAEVGYHMDTGLKSPGFAFADEGARGDTWHALLNGIYLL
                     DRGALWDTGNLVLELSYDRLDDVTRNEELFVRVDHGNLCTVGRGGPAGSWKDGCATKD
                     AWGLSIRFAPQWLQVLPSLDVSLPMSYQVGLDGNSAISGLGVNQEAASLSLGVQLDYK
                     VIHRLTLEYADYFAKRHTVDDVAVSGNGSSFSLTDRGRVGITYKVAF"
     gene            7999..9066
                     /locus_tag="PLW13_03265"
     CDS             7999..9066
                     /locus_tag="PLW13_03265"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY69418.1"
                     /translation="MKSIMCSTFAVCLVSLFMAAGVAAEDSFVDPLEAPAEASPLAQH
                     SQLLAIARAGSRLVVAGMRGHILYSDDEGRTWSQASVPLSVTLTALSFPTPQQGWAVG
                     HSGVVLHTADGGQNWERQLDGRRTPADMIGFYEGLAAGGDEVAARLVQELPVNWQDGP
                     EQPWLGVWFADGQHGFVTGAFNLMLETRDGGNSWTPWTHRLDNPEALHLNAIERIGTA
                     FYIPSERGVVFRMRDGEERFSALQTGYAGSFFGICGDERVLLAYGLRGTLYASQDQGE
                     TWSALPPAIPVALTACTRAADGRFLLAAASGHVVAVAPGANGPVATPRDGAPWPLAGI
                     ALAADGKPLGVGMRGIWRAEQ"
     gene            9121..11523
                     /locus_tag="PLW13_03270"
     CDS             9121..11523
                     /locus_tag="PLW13_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQY69419.1"
                     /translation="MSAKIEKIDLPVIRDPADFDDKSGDALERLIFNHRPLVLLLVAL
                     VTLVLGYQATRLEINANFEDMIPQSHPFIKNFFAYRDSISGLGNSLRIVVENRDGDIF
                     DKDYLEVVRQVNDRIYLQPGIDRAWMKSVWTPATRWTEITEEGYRGGPVMPFDYNGSP
                     ESVTQFRVNVLRAGLIGRLVGSDYKSSMIVVPLLERIPETGEPIDYAALSDFLEHEVR
                     SLQTDKYRIHVIGFAKLVGDMIDGIRSVLIFMAFSALIATLIIYVYTRDARSTLLLVG
                     AAVLGVLWLLGMLQLTGYVLTPYSVLVPFLVFAIGLSHGAQKMNGIMQDVGRGTHRYV
                     AARYTFRRLFVAGLTALLTNAFAFAVMLLIDIPAIRDLAITTTMGVLILIFTKLILIP
                     VALSYIGVGEKAARRSLETGSATGENGLLGRVWAMIGRCSERRYAIPLVVASIALGIG
                     SFAISHAYLQIGDLDAGAPELRPDSRYNQDVAYINAHYRISGDQFAVMTVTGPEGIRD
                     YETLVEQDRLAWTLQQLPEVIAVESLGSSVPLITMGTNEGNPKWMDIPRSRQVASLTV
                     NTATTNAPELLSGDATVAPVVAYLRDHKAETLARVARVAQDFADEHGSEQRQFLLAAG
                     SSGIDAATNEVVAVANYRMLALVYASVIVLCYITFRNWRAVIVALVPLAITSFLCEAL
                     MVVLGIGIKVSSLPVTALGVGCGVDYALYLVTVQLAAQRSGRSLGDAYRDALNFTGRV
                     VCLIGITMGASVITWAWSPIKFQADMGILLAFMFVWNMVGALLLIPALSHFLLRTQRD
                     AR"
     gene            complement(11683..12975)
                     /locus_tag="PLW13_03275"
     CDS             complement(11683..12975)
                     /locus_tag="PLW13_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HQY69420.1"
                     /translation="MRDIYWFRDDLRLQDNPGLLEHAQAEELLCVFFWPRPRPWCNVT
                     GLGAQRERFLLESVQALREALRARGQELLVLHGAPELLLPELAGQHRIARIGVAHTGG
                     VDEQRQLERVRERVGIPLELHPPNTLFRQEQLPFVPGEMPRQFTPFRERVEALEVNAV
                     HPVPDALPPPPRGARFAALSRAVASPHPGFTARGGTAGGEQRLHDWMWRERAVARYKD
                     TRNALDGLHASGRLSPWLANGSLSVRQVALELFRFEREETRNESTRWFWQELLWREFF
                     HWRARVDGVRLFAPGGIGGRRALCCFEPRAFARWCAGDTDHPLVNALMRQLVQTGWMS
                     NRGRQIAASCLVNELGLDWRYGAAFFEKHLIDYDVASNYGNWQYIAGVGCDPRGGRHF
                     NLAKQAATFDPDGEFTRAWGGLRPAQPRHVVDAADWPI"
     gene            complement(13048..13362)
                     /locus_tag="PLW13_03280"
     CDS             complement(13048..13362)
                     /locus_tag="PLW13_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69421.1"
                     /translation="MKKLIIRSWDAVMDNRHNPLRHLDPASQHFIMQVLGWMWSMVFS
                     LSFLSIFYFSMVWMAHLLLLGGVALTVATFRQAENQSLERNSRLRAGTAPACVWMLDK
                     EA"
     gene            13597..14127
                     /locus_tag="PLW13_03285"
     CDS             13597..14127
                     /locus_tag="PLW13_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY69422.1"
                     /translation="MVRTAFALALAGAATFARADCPALLDHEVRVLRKSDIVNLCDRY
                     AGQPLLVVNTASHCGYAPQLQGLETLYQEYRARGLRVAGFPSNDFGQEARKEEDTARI
                     CYVNYGVTFDMYSETHVKGARAHPLFAELARQSRAPRWNFNKYLVDGDGKVVAAFGSG
                     TSPDSPELREAVEKLL"
     gene            14263..16005
                     /gene="argS"
                     /locus_tag="PLW13_03290"
     CDS             14263..16005
                     /gene="argS"
                     /locus_tag="PLW13_03290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQY69423.1"
                     /translation="MNLRDHLNAAVEAAMRAVGIPADCQALLTQSTRPEFGDYQANGA
                     MGAAKRLGTKPRELAERIVAAFDGAGMVERLEIAGPGFINIHLAPAFLAARVEAAATD
                     ARLGIPASEQPQTVVVDYSAPNLAKEMHVGHLRSTIIGDAVVRALEFRGERVIRQNHV
                     GDWGTQFGMLIAHLEDVMATGQSIDDAALADLEGFYRSAKQCFDADPAFADRARDYVV
                     RLQSGDAHCRRLWQRFIDISVAHSEEIYRKLNVTLTHADIKAESAYNDDLPVVIAELD
                     QRGLLAESEGARVVFLEEMRDRDGNAMAVIVQKGDGGFLYATTDLAAARYRAQVLHAD
                     RILYFIDDRQKLHMQQVFAISRRAGFVGESTTLEHHPFGKMLGEDGKPFKTRSGGTVK
                     LAELLDEAVERAARLLAEREADLSAQERAVVARQVGIGAVKYADLSKNRTSDYVFSWE
                     HMLSFEGNTAPYLQYACTRVRSIFRKAGIDTAALTGPIGIDAPEERALALRLAQFSEA
                     LSSVTHDAAPHLLCAYLYDLAGLFMRFYENCPILRDDVPPAVRASRLRLADVVARTLA
                     TGLELLGIETPTRM"
     gene            16129..19920
                     /gene="putA"
                     /locus_tag="PLW13_03295"
     CDS             16129..19920
                     /gene="putA"
                     /locus_tag="PLW13_03295"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HQY69424.1"
                     /translation="MMNLPDLAEDELRRRIRALARADEGERVAALLAEADAALGDGDA
                     ITATATGLIEAIRGKQRYRAGINALLAEYSLSSREGVVLMCLAEALLRVPDRLTADRL
                     IRDKLGGGDWRAHVGNSQSLFVNASAWGLLLTGKVVQLREQDAGAAWRMLGHTVGRAG
                     EPAIRAAMRLAMRIMGTQFVLDTDIAAALDKAREWEDRGYLYSYDMLGEGARTSADAH
                     EHFLAYRGAIDAIGRRAGAAAPAAAPGVSVKLSALHPRLELAQTERVRAELVPRVLEL
                     AVAARAHNIGLTIDAEEAERLDITLEVFEAVYRDPQLTGWEGLGLVVQSYLKAAPAVI
                     DWLAALAASHGRRIMVRLVKGAYWDAEIKHAQTIGLACYPVYTRKSSTDLAYLVCAAR
                     LLARRERLYPQFATHNAHSAAAVLALAGSPGGFEFQRLHGMGEELYEELVERRGVPVP
                     VRIYAPVGVHRELLAYLVRRLLENGANSSFVHNLADDALASTTLAADPRARLRGLSHY
                     ANRGIALPPALFGATRANARGVDLRDYEALHGFAAGMDAWRASAARLGCAHGPGCIPV
                     VNPADHREVLGHYLPSSEHDIEQAAARAHAVAAGWAATPFAERCGYLRRAAGLIEDAL
                     PELAAFCMKEAGKTLGDALAEVREAIDFCRYYAAQAQTLLGAHGHALAARGPILCISP
                     WNFPVAIFTGQLAAALVTGNPVIAKPAEQTTLVALRVAQLFHEAGVPREVLQLLCGPG
                     EPIGARLLPDARIAGVMFTGSTAVARHIALQLAARGGARAMLIAETGGQNAMVVDSTA
                     LPEQVVDDVIASGFNSAGQRCSALRVLYVQHEIADRLINMLIGAMSELRIGDPAHAAT
                     DIGPIIDHRARARLQAHVERLAGRARVLHAGKLSADCDEGSFFAPHLFEIDSIEQLPR
                     EVFGPVVHLIRYRAADLEHLPRRINAAGYGLTFGIHSRIQGSIDLLAREIAAGNVYVN
                     RNMIGAVVGVQPFGGRGLSGTGPKAGGPLYLGRLLRAVAAQPPAYRPLPEETAGDLPQ
                     AFDCRAAVHALAELQQEWRHSDLHARLAPARALHERIAHDPSREHDARGELAAQLQQL
                     LQQAGAIGQPRALPGPTGERNTLRLDARGVLLCVCREGAGERDALLPALAALLAGNAV
                     LFLTDAATGAPVLAVRNLLAEAGFADGLTAHHALPDVAAMQAVITACAIDGVLCHGDP
                     ALAQRLSHDLAARAGALLPLIDDDFGPLYLERFVQEKTISVNITAAGGNAALMSQAES
                     D"
     gene            19920..20666
                     /locus_tag="PLW13_03300"
     CDS             19920..20666
                     /locus_tag="PLW13_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY69425.1"
                     /translation="MMFDTEGVVLAALIFVAAMLYASVGHAGASGYLAAMALFGVAPE
                     VMKPTALSLNILVALIATFKFYRAGAFSWRIFLPLAAGSVPLAWLGGTLVLPGHFYKP
                     LVGMVLVYAAWASLKRASRPQDAQLRTPPTVLLIACGAVLGLLSGLTGVGGGIFLSPL
                     LLFLRWAEVRVISGIAAAFILLNSIAGLLGVIRVAPELPAALPLWAVAAIAGGFLGAE
                     LGSRRLGNAGIQRALALVLAVAGVKMIATA"
     gene            complement(20677..22809)
                     /locus_tag="PLW13_03305"
     CDS             complement(20677..22809)
                     /locus_tag="PLW13_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY69426.1"
                     /translation="MERRQFLKLGATATGGMILGLHLPQSRAAGVAEEPVQMNAWIRI
                     DPDNRITFICHRNEMGQDVHTSLAMLVAEELQVPVASLGIEQAPVAAVYTNAMIGAQI
                     TGGSTSIRDAWLPLRRAGATVRTVLLEAAARTWQVDVGVLEARDGYVHNRNGNKLSYG
                     FLSGIAADLPVPAPEYLALKAPGAFTQIGSATQKRLDTRAKVHGERLFGLDATQPGMV
                     HAALTQCPVIGGTVASVDAAAARAMPGVRDVIDIGEGVAVVADHFWIAKQARDALKIS
                     WNLGAGTAVSDASVAAALRAGLKETGAVARANGDIDAGLARATKRLEADYELPLLAHV
                     AMEPMNCLARVDDEGCDIWTSTQYPAGARAVAAARTGLAPERVRIHSQFIGGGFGRRL
                     DVDFIAQAAAIAKAVPGVPVKLVWTREDDTTHDFYRPASLHRLRGGLDAKGKLIALDH
                     LMVSQSVTERAFPGVVKDGLDPFMTEGASNLVYAVPNLRSRVVIRDTGVRVGYWRSVS
                     HALNAFAFESFIDELARSGKRDPLEFRLEMLAEQPRQRAVLEKVAAMAQWERSRGADA
                     ALGIASMECYGSYIAMTAEVQRRGKDVHCAHIAVAVDPGIAVRPDQVVAQIESAVITG
                     LTGALRNRISIVDGVVQEQNFHQFQPLRMSETPQIEVAIVASGDAPGGMGEVGTPLVA
                     PALANAVARLTGTRVRKLPFSEAGLRFV"
     gene            complement(22813..23271)
                     /locus_tag="PLW13_03310"
     CDS             complement(22813..23271)
                     /locus_tag="PLW13_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY69427.1"
                     /translation="MLQLTINGREHRVDAAPDTPLLWILRDHLDMPGTKFGCGVALCG
                     ACTVHIDGQPARACATRAADAAGKAITTIEGLDGKVAQAVQQAWEALDVPQCGYCQSG
                     QIMSATALLAHNPRPDDAAITAAMDGNICRCGTYSRIRQAIRNAATALEG"
     gene            complement(23319..24716)
                     /locus_tag="PLW13_03315"
     CDS             complement(23319..24716)
                     /locus_tag="PLW13_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69428.1"
                     /translation="MSETSNDAKRQLRAAWDEMIESLQQARDGIDVPQLMPAPPTERN
                     LAEGYRYLMGFVHHGIERAFHEDPVRPHFHNALSIFNRSTIDNPDAIYFYAPIDGSKS
                     YLLRGEMGDARHWKGEAAAPTGRKAPHYLIFEASTGGLSGDTGDLSELRPGMKLQTGR
                     LDCSRIEVNPDGSFEILFAPERPAGFAGNYICTLKVEHRPHPADPTMPPERYASYISG
                     RQLFYDWAREDAIHFEMTQLGAEGTHPEPYTPVRAAADIRRFGQLVRGQMHFWNAFWT
                     IPMGVYGERKGSMPGIAMPRNAFNTINAASGATGGGMSTNLYAGGVFDLGPDEALVIE
                     SRIKVAPRYIGFQLGNLWGESLEYASQITSLNGWQSHVDPDGVIRLVVAHRDPGVPNW
                     LDTAGHAQGFLSPRWAYSEQPPQDQWPEITATKVRFDEIRAHLHPSTPTVGPDQRRRE
                     IEMRQRHVRKRYRVF"
     gene            complement(24923..26644)
                     /locus_tag="PLW13_03320"
     CDS             complement(24923..26644)
                     /locus_tag="PLW13_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY69429.1"
                     /translation="MRPVSSELAGWKRWLPGVQMLARYEPAWLAHDLMAGLVLTTMLV
                     PVGIAYAVASGVPGIYGLYATIVPLLAYALFGPSRILVLGPDSSLAALILAVVLPLSG
                     GDPMRAVALAGMMAVVSGLVCILAGLLRLGFITELLSKPIRYGYMNGIAFTVLISQLP
                     KLFGISIDSDGPLRDLWHIGEALLGGAANWTAFAIGAATLALIFLLKPWQRIPGILIA
                     VAGATLAVGLFDLGQTAGVKVLGALPQGLPAFALPWVGLADLGAVVIGGCAVALVSFA
                     DTSVLSRTYAARTRSYVDPNQEMIGLGAANLAAGFFQGFPISSSSSRTPVAEAAGAKT
                     QLTGVVGALAVALLLVFAPDLLRNLPTSALAAVVIASAIGLFEFADLRRIFRIQRWEF
                     WLSIGCFVGVAVLGAIPGIGLAVVAAIIEFLWDGWRPHFAVMGRVDGIRGYHDIKRYP
                     RARRVPGLVLFRWDAPLFFANAELFRERVLQAVADSPTPVRRIIVSAEPVTSVDVTSA
                     DVLAEMEQTLRESGIELHFAEMKDPVKDKLRRFELMEHFGPDVFHPTLGAAVDDYLAD
                     HAVDWQP"
     gene            26861..29092
                     /locus_tag="PLW13_03325"
     CDS             26861..29092
                     /locus_tag="PLW13_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY69430.1"
                     /translation="MRHAFKAFLLLALATTMAPQFASAGPEAAPRVGLVLAGGGARGL
                     AHIGVIKYLEENNIKVHAIAGTSMGSVVGGLYASGMNAAQVEEVARTLDWRFAFNDST
                     AREQQSFRRKQEDFDFLIGARLRFKDGRLSIPMGAIEGQHLNLLLHDLVRHVSGIDDF
                     DKLPIPYRAVAADVVTGDAVVLDRGDLAVAMRASMSIPGVFAPIDLDGHLLVDGGIAK
                     NIPVDVVQGMGVDRLIVIDIGTPLAQREELGNVLSLIGQLTTIMTRKNSEQQLALMRE
                     GDVLILPALDEAGVQTMSFDRVDDAIRLGYEAASALAPQLAAFAAPGPAQQVAGITEP
                     GPPRIDATRVETDANVSKQLLRSRVTQPLGEPLDRELLERDIAEIYGFETFSRVDYQV
                     QPTAAGNELVVRATANPGGVNYLKLGMSWDQDSHGTSEFGLRASWRQRGINDLGAEWF
                     TYGQVGGNSNFGTEFYQPLDQLQHYFVEGRYRFDQRRLNLSRNARVLARALVDGHQVD
                     LAPGINLGKVAQWRAGVYAGTANTDVEIGAPELSSRSDNDGGYFTELGYDSLDRPYFP
                     GSGLRLLSRYSWSDGSLGASEDYEAWNTSAFGSISLGRNSFTAIGRWAELDLDGSGDS
                     LTGSNVFLSRAYTLGGFLALSGYTSDSLAGNYLGFAGLTYYRRLTEQSLLPVDMPVYA
                     GASLEAGNTWLDESDASFDDLIYAGSLFLGVDSPVGPIYLGVGMAENSQYAMFLKIGQ
                     IFD"
BASE COUNT         4694 a        10013 c         9818 g         4689 t
ORIGIN      
        1 gccctgcgtc ctgatgacct cgaagaatcg tggccctgcc tggtagccga ccgcgaatat
       61 gaacagggca aagccgaccg actgcgcccc ttcgctgatc ttgaacccga actgcccgag
      121 cagcagcgac accagcaacg cacccgccac gggacccagc gaaatcgagg caatgcgcaa
      181 ccggccgacg agataaccgc agccgagggc gaggaacaac gtgaataccg ggttgaccag
      241 cagaaaatcg cgcaaaacag atgcggattg catgggcact ggctccaccc ggtcaacggt
      301 cgatcaggca accgtcctcg atcgtcaatc gaaattgtat ttgaacgaca tctgcaagcg
      361 ggtgtcgtcc gcctcgctgc catccctcag ttcgagttcg ccgtagacca gctcgccgcc
      421 gaccatgacg cccggcaccg ggtagtagag caggttcagg ttgccgtacc gggcgccttg
      481 ataggcgctg ccgaactggc catcgctgtt gtcctgctcc gcgacgctcc agccgatgct
      541 gctgctccag ttctcgctcc agtagtggtc gtaatagacc agcgcgccga gcagcggcag
      601 cacttcggcc tcgtcgagat cgccgttcgg cgcgaggtcg ttgccgccgt cgttgatata
      661 gctggcgatg ccctcgccat aggcgagctg caggtgcagc ttgtcgcgcg cgccgaacac
      721 gatgctcgtg ctggcattca ggccccagcc gagttcgtca cccgagggat cgccgttgcg
      781 gctgctctcg gtctcgtagc ccaccgagcg caggatgccc gcgagttgcg catgccccca
      841 gtcaccgctg gcgcgccact gtgccgtgag atccggcagg ttgttctcgc tccggatgga
      901 cagcgggttg tcctcgggat cgatgtcctg gaccttgccg gtatcgagcg ccgaggaggg
      961 cgactcgatg gcgatcgcga actgcgatcc cttgccgcgg taggccgtcc agcgcagctg
     1021 cgggttgcgc aggaacatca tgccggtcgg cccccagtaa tcgatggtgt tcgggaacac
     1081 gtcgggatcc atgaacagcg accaggtctg gcccgcgccg aaatcgccga gctcggccca
     1141 ggcatggcgc agccgcggcg tggtctggcc cgcatcgacg ccgacaccga agagctcgaa
     1201 ttcgagccgg gtgttcagtt cgccaagcgc ggtcggcgtc gtgccgcgga tgccgatgcg
     1261 cgtctggcgc acgctgaaga tggtttcgcc gtccttgccg cagcccgcgt cgccgggaca
     1321 attgaccggg atcttcgaca cgcgcagcgt gtccgtccag tccggatccg actggttgac
     1381 gtcatagatc gtatcgagct ggatgaagcc gtagatgtcc agcttcgcat cggccgcgct
     1441 gcgtgcggcc ggactgcttg ctggcgcggg agcggccacg accgcggtgg cgctgcccgc
     1501 cggcggcacc gtctcggaac tgaggccagc gggagcggcc tgcgcggatt cgagctggcg
     1561 gatgcgttgc tcgagccgct cgatcgcgac gcgcgaagcc tcgagctcct ggcgcagctc
     1621 gctgatgtcg tccgcgcggg cgagcgaggc gcaactcacc gcaaccgcga gcagcagacg
     1681 cgggaaaatg ggccgggctg cgcggcggat gtatggcgtg ttcacgtggg agctcctgct
     1741 tggaacaaat gaatcatcga aggcggacgc ggttcgtgct gccgtgcaca cctgttttcc
     1801 gggccgccga ccgggatgct cgccggcggc ggccacgatg ccgtgcttat ttgcgcaccg
     1861 ttaccgtgta gggattgccg cccagcgctt tcgatatgtc agcgatggcc ttctgcgcgc
     1921 gcacgctgtt gcctgccgca tcgaggggcg gcgagatcac ggcgatgccg aagcggcccg
     1981 gcgataccgc gatgatgccg ccgccgacgc cactcttggc gggcaggccg gtccggtaca
     2041 gccactggcc ggaatcgtcg tagagtcccg ccgtggccat cacggcgagc acgttcggca
     2101 cataggcgct gttcagcacc tgcttgccgg tgaccggatt gcgaccgcca ttggccaggg
     2161 tcgccgccat cgtggcgaga tccttcgcat tgacgttgac cgaacactgc tcggtgtaga
     2221 tgtcggtggc ctggtcgggg ttttccttga tatggccgta ggcatacatc aggcggccga
     2281 tggcctggtt gcgctggttg gtcgccgcct cggattcgaa gacttcctgg ttgaccgaaa
     2341 gcttgcggcc ggcgaaatcg ctgtagtagg cgaggatctt gttccagatc tcctcgcgcg
     2401 aagcgcccgc gaccatgctg gtggcggcga tggcaccggg attcaccatc gcgttcatct
     2461 cggcgccctt gtgctgctcg atcgcgacga tcgagttgaa gacctggccg gtggcatcga
     2521 cgcccatgcc cccggagatg gcatccggac ccttctcctc gatcaccttg gccatggtga
     2581 agaccttgga gatcgactgg atcgagacct cggagctcac gtctcccttg gtgtatacct
     2641 tgccgtcggc agtcaccagc gcgatgccgt acagctcggg atcaaccttg gccagggccg
     2701 gaatgtaatc ggcgttcttg ccttcctgca ggccccggta cttctcgtag gccgcatcga
     2761 gtgccgcgtt gatcgccctg ggcgacaact ccgccgccga cgcccccgtc acgccgagga
     2821 cgaataccgc cagcgcggca atgatcgtga cgctgaatct tgctgtcatc gaaggctcct
     2881 gtaccagagg ttttttccac tatatacacg cgcaatagcg actttcaacg cctgcaggca
     2941 gcggacggtt gccggcagtc gggccgttcc ggagctgccc gcgccggcaa tgcacaatgc
     3001 cgtccgctcg atcgcaccac ggtgttccac cggtacctcg gggctgcgct tgctgcaatc
     3061 gttgttcatt ctcagcggat cccccgcccg cccaggcgcg ccggcgtcat ctgggcgtcc
     3121 ggcaggtatg gcatgccgcg gcggtcgtag cagcgatcgc acagatggaa ctgcatgacg
     3181 gcgtactggc cgcggaccag atcgtaggcc tgcacggatt ccgccatgaa atagccgccg
     3241 tgggcatagt cgggcaagcc gttgcccagg accacccggt agagcttgcc ggactgatcc
     3301 cagccctcgt aggccagtgc gacccacgag tcctcgtcga tgtagaaaca cttgcgcgag
     3361 gccatgtgcc gcgcgccggg caggggcgtc gcctcgacca cccacacgcg gcgcaactcg
     3421 acgcgtgcct gttcgggctt tacatgttgc gggcccagca gctgcgccgg gtccccgcgc
     3481 agcatgtgca tgcggtagct gttgtagggc atgtacatga gcttgcggcc cttcaactgg
     3541 aagtcgaagc ggtccatgcg ccccgcgaac agctgcacct cgtcgtagaa cagcgcaccg
     3601 cctatgtgcg acaccggggt gtcgtaggaa aactccggcg ccatgcgcac ccggcgttga
     3661 cccgcctggt agctccacgc gcgctggtcg tccacatcaa agtgcaacgc gaaccacacc
     3721 agcgtctgcg agccggcctc gcgcacgggc aggatattcg tggccagccc acgcaggtag
     3781 tagaggcccg ctccctggta gggctcctgc tccgggttgt aatagggctg atcgccgatt
     3841 gcgtggttga tgacgagcag ggagatcccg ccgttggcgt tcaccaggta ggaatccgcg
     3901 gtcaattccc cgggggccgg gtagaacagc aggtggttcc acatggcctc gtagccggtc
     3961 ttggggatcg ggaacggcgt cccgccccag cacccgtaaa cgcccacgcc gttcgcgctg
     4021 gtcttgcact cgggattgcg cgcgttggcg agcgagttct gccggatccg ctcgggtagc
     4081 cagaagccgc ggcgcgtcgg gtagatgtcc aggcggtaac ccgggtagcg gcgcatcagc
     4141 tccatctgcc cttccatgag ccggtcctgg tactgcgcca tgttgctcgc gtcgatcgag
     4201 atcagcggct tgtcctcggg atagggatcc ggccagcggc cgctggccgc atcgaatccc
     4261 ggcggtgccc tggtgccgcc ggtccaggcg gggtagctgc cgtcggcctc gccggtgatc
     4321 tccgcgcccc agggcgtcag cgtcgtgccg agccgctcgt actcttcctg cgtcaccagc
     4381 gcatgcgatg ccgcgcacat caggccgagt gacacgcgcg cggcgattgc gccgatcgct
     4441 tgcatgaagg ttgccatggc tccctccgct gttattgttc tgctttcttt cctgccgtgc
     4501 gttcctccaa ggcgcgaacc cgagagcgcg gcgcgcgatt gctccctcag cggatgcctc
     4561 gctgcgtcat gctcgccggt gacagttcga cctccggcca gtacggcact tcgttacgca
     4621 cgtaggagcc gtcggccagc tgcgtctgca gggccgtgta ctgtccacgg ctcacgtcat
     4681 aggactgcat cgacgaggca tagctcagga agccgccgtg tgcgtagttg atcgcgccgt
     4741 tgctgaacat gacgcgaaac agcttgttgg agtgatccca gccctcgtag gcgatgaccg
     4801 cccacgtgtc ctcgtcgaga tagaagcgct tgcgggaggc catgtggcgc acgccgggca
     4861 gtggcgtggc ttccaccacc cacacccggc gcagctcgtt gcgcaccatt tccggcttga
     4921 agtgatgcgg gcccagcaac tgggccggat cgccgcgcaa cagctgcatg cgatagttat
     4981 gataaggcag gtacatcagt tgccggccct tgagctcgaa ctcgaagcga tccatgcgcc
     5041 cctggaacag gctgatctcg tcgaagaaca tcgtgccgcc catctgcgcc gagggcgtgt
     5101 cgtaggcaaa ttccggcgcg agccgcacgc gccgctgccc cggcgtgtag ctccacgcgc
     5161 gctggtcgtc cttgtcgaat tcgagcggcc accacagcat ggtctgcgtg ccggcgtcgc
     5221 gcgcgggcag cgtggcgatg ttatggacgc gctggtaata cttcccggcc ccctcgtagg
     5281 gcgtgacatc gggattgttg tagggaaact cgttgtaggt ccgggtctgg accagcaggg
     5341 tgatcgcgcc gttggcattg acgaggtaac cggtggtcac gtagtcagag gtcgtggaac
     5401 tgcgcagcgc atggttccac atcgcttcgc ggccattcct cggaatcggg aacggcgtgc
     5461 caccccagca accgtacatg cccaccccgt cggggctggt cttgcactcg ggattgcgcg
     5521 cgttcttcag cgtcccttcg acgaagcgcg gcggcatcca cacgccgcga tgcgtggggt
     5581 agatgtccat gcgaaagctc gggtagcggc gcatcagctc catctgccct tccatgagcc
     5641 tgtccttgtg ctgctccatg ttcgtcgcgt cgatggagaa cagcggtgtc tcgtcgggcc
     5701 agggatccgg ccacagaccg ctctgcggat cgaatcccgg tggcgccttc aggtcgccgg
     5761 tccaggcagg atagctgccg tcggggctgc cggccttctc cgcgccccag ggcgtcagtg
     5821 tcgtgcccag cagctcgaac tcctcgtccg tgagcgccgc ttgcgttgcc gcgcatatca
     5881 atcccagcgc gatacccgtg agacaggccc cgattttctt ggtgttcatg ccatcagctt
     5941 cctcaatgcc ctgcgttttc gcttatatgc aaacactgtt cctccggtgc gccgcgcaat
     6001 ccggaaaacg cgcgtgccgc aactcgtcgc cacccggctc ggatgcatcg cgcagtgatg
     6061 acagtaagcg aatttccgta acaacctttg cgatggatca aggtagttgc gcgaggctag
     6121 agaatagatt caccaacgtg atgtccgacc ttgtttttgc gcgctgacag cggcaggttg
     6181 cagacagcag gtagcgcccg tgtggagttg cgcctgttca cttcaaacca taaatccggg
     6241 ggtggccagt ggattcgagc atgcatttca gtcgcctgtc cgtgcgggcg ctgcgccgcg
     6301 gcgcattggc catcggtatc gcggccgcgg ggatcagcgg gggcgcatcc gcgttcagcg
     6361 tcgataccgc aaaccccgat ctcgagatca cgtgggacaa caccatccgg ctcaacgcgg
     6421 gctggcgggt cgaggatatc gacgacgcgc tgggcgacaa cttcacctac gacgagagcg
     6481 actaccggtt cgaacaaggc gacatgatcg cgcagcgggc cgacctgtac tccgaactcg
     6541 acatcgtctg gcgcggcaac tacggcgcgc gggtatcggc cgcgggctgg tacgaccacg
     6601 cgtaccgcga cgacgaggtc gagcagaacc ccctgctcgc cgacctgccg agcagctatc
     6661 gcaacaagga gtacacgggc ctgaccgagg atttctaccg cggcccctac ggtgaagtgc
     6721 tggatgcctt cgtgttcgcg aaccagacca tcggcgaagt ccccgtgtcg ctgaaggccg
     6781 gacagctcac cacgtactgg ggcatggcgg tgttctatgc cggcgggatc gcgcagagcc
     6841 agcacccgat cgacggacgc aagggtgtcg cgaaccccgg cagcgaggtg aaggaattgt
     6901 tcctgccact gtcgcagatc aacctgcagg cgcaactcac cccgacgatc gcggccgaag
     6961 tgcagtacta cctggactgg gccaacacgc gcgcggccga ggggggcacc ttcctcgcgg
     7021 cagccgatct tgccttgcag gggccgcagc agctgggcgg cgcgggagca gccaacctgc
     7081 agcgccgcgc gccgctgcag ccggacgatc agcacggcaa ctggggcgtg aacttcaagt
     7141 tcaacccgga cttcctgcgc ggccagacgc tcggggtcta ttaccgcgaa ttcgacgaaa
     7201 aggtcccgtg gctattcctc acgctgcccc ccgccgagtt tggctatcgc gcggtgtacg
     7261 cggaaaacac caagctggcc ggcatcagct tcgacggctc gctcgggcag tgggccgtgg
     7321 gcgcggaagt cggctatcac atggatacgg ggctcaagtc gcccggtttc gccttcgccg
     7381 acgaaggcgc gcgcggcgac acctggcatg cgctgctgaa cggcatctac ctgctcgacc
     7441 gcggcgcgct ctgggatacc ggcaacttgg tgctggagct gagctacgac cgcctcgacg
     7501 acgtgacgcg caacgaggag ctgttcgtgc gcgtcgacca cggcaacctt tgcacggtgg
     7561 gacgtggcgg cccggcgggt tcgtggaagg acggctgcgc gaccaaggat gcgtggggcc
     7621 tcagcatccg cttcgctccc cagtggctgc aggtgctacc cagcctcgac gtgtcgctgc
     7681 ccatgagtta ccaggtcggg ctggacggca actccgccat ctcgggcctc ggcgtcaacc
     7741 aggaggccgc atcgctgtcg ctcggcgtgc aactggacta caaggtcatc caccgcctga
     7801 cgctcgagta cgccgactat ttcgccaagc ggcacaccgt ggacgacgtc gccgtcagcg
     7861 gcaacggcag ctcgttctcg ctgaccgatc gcggccgcgt gggcatcacc tacaaggtgg
     7921 cgttctgatc gatactggag cagagtggag cggacgacgg ggaaccagga ccggaaatca
     7981 gggcgcaaga acataagaat gaagtcgatc atgtgcagca ccttcgcggt gtgcctcgtc
     8041 tcgctgttca tggcggcagg ggttgcggcg gaggattcgt tcgtggaccc gctcgaggcg
     8101 ccggccgagg cgagtccgct ggcgcaacac agccagttgc tcgcgatcgc gcgcgcgggc
     8161 agccggctgg tggtggcggg catgcgcggg cacatcctct actccgacga cgagggccgc
     8221 acgtggtccc aggccagcgt gcccctcagc gtgacgctga ccgcgctgtc cttccccacg
     8281 ccgcagcagg gctgggccgt ggggcacagc ggcgtggtgc tgcacaccgc cgatggcggg
     8341 cagaactggg agcggcagct cgacgggcgc cgcacacccg cggacatgat cggcttctac
     8401 gagggtctcg cggccggcgg cgacgaagtg gcggcgcgac tcgtgcagga gctgcctgtc
     8461 aactggcagg acggcccgga gcaaccgtgg ctcggcgtct ggttcgccga cggccagcac
     8521 ggcttcgtca ccggcgcgtt caacctgatg ctcgaaaccc gcgacggcgg caacagctgg
     8581 acgccgtgga cgcatcgcct cgacaacccc gaggcgctgc acctcaacgc gatcgagcgg
     8641 atcgggactg cgttctacat accctcggag cggggcgtgg tattccggat gcgcgatggc
     8701 gaggagcgat tcagcgcgct gcagacaggg tatgccggga gcttcttcgg catctgcggc
     8761 gacgagcgcg tgctgctcgc ctacgggctg cgcgggacgc tctacgcgag ccaggaccag
     8821 ggggagacgt ggtcggcgct gccgcccgcc atcccggtcg ccctcaccgc ctgcacgcgc
     8881 gcagcagacg gccgcttcct gctcgccgcc gcgtcggggc acgtcgtcgc ggtagcgccg
     8941 ggtgcgaacg gcccggtcgc cacgccccgg gacggcgcgc cctggccgct cgcggggatc
     9001 gcgctcgctg cggacggcaa gccgctcggc gtcgggatgc gcgggatctg gcgcgcagag
     9061 cagtgaaccc ggccgctccc ggctgacccg gcccatgaca acaacaggat tcgcgacgcg
     9121 atgagcgcca agatagaaaa aatcgatctg ccggtcatca gggatcccgc cgacttcgac
     9181 gacaagtcgg gcgacgcgct ggagcggctg atcttcaacc accgcccgct cgtgttgctg
     9241 ctggtcgccc tggtcacgct ggtgctgggc taccaggcca ccaggctcga gatcaacgcg
     9301 aacttcgagg acatgatccc gcagtcgcac ccgttcatca aaaacttctt cgcctatcgt
     9361 gacagcatca gcggcctggg caattcgctg cgcatcgtgg ttgagaacag ggacggcgac
     9421 atcttcgaca aggactacct cgaggtggtg cgccaggtca acgacaggat ctacctgcaa
     9481 ccgggcatcg atcgcgcgtg gatgaagtcg gtctggacgc cggccacacg ctggaccgag
     9541 atcaccgagg agggctaccg cggcggcccg gtcatgccct tcgactacaa cggctcgccg
     9601 gagtccgtga cccagttccg cgtcaacgtc ctgcgcgcag gattgatcgg caggctggtg
     9661 ggctcggact acaagtcgag catgatcgtg gtcccgctgc tggagcgcat ccccgagacc
     9721 ggcgaaccga tcgactatgc cgcgctgtcg gactttctcg agcatgaggt gcgcagcctc
     9781 cagaccgaca agtaccgcat ccacgtgatc ggattcgcga agctcgtcgg cgacatgatc
     9841 gacggcatcc gctcggtgct gatcttcatg gcgttctccg ccctgatcgc gacgctgatc
     9901 atctacgtct acacgcgcga tgcgcgcagc acgttgctgc tcgtgggcgc agccgtgctg
     9961 ggcgtgctct ggctgctcgg aatgctgcag ctgaccggtt acgtgctcac tccctattcg
    10021 gtgctcgtgc ccttcctggt gttcgcgatc gggctgtcgc atggcgccca gaagatgaac
    10081 ggcatcatgc aggatgtcgg gcgcggcacg caccgctatg tggctgcccg ctacaccttc
    10141 cggcggctgt tcgtggcggg tctcaccgcg ctgctaacga atgccttcgc cttcgccgtg
    10201 atgctgctga tcgatatccc ggccattcgt gacctggcga tcacgacgac catgggcgtg
    10261 ctgatcctga tcttcaccaa gctgatcctg atcccggtcg cactgtccta catcggcgtc
    10321 ggcgagaagg ccgccaggcg cagcctcgag acggggtcgg ccaccggtga aaacgggctg
    10381 ctcggcaggg tctgggcgat gatcggccga tgctccgagc gtcgctacgc cataccgctc
    10441 gtcgttgcga gcattgcgct cgggatcggc tccttcgcga taagccatgc gtatctgcag
    10501 atcggcgacc tcgatgccgg cgccccggaa cttcgccccg actcgcgcta caaccaggac
    10561 gttgcctaca tcaatgcgca ctaccgcatc tcgggtgacc agttcgcggt gatgaccgtg
    10621 accgggcccg aggggatccg cgactatgaa acgctggtcg agcaggaccg cctcgcctgg
    10681 accctgcagc agctgccgga agtgatcgcg gtggagtctc tgggcagcag cgtgccgctg
    10741 atcaccatgg gcaccaacga aggcaatccc aagtggatgg acatcccgcg cagcaggcag
    10801 gtcgcgagcc tgacggtgaa cacggccacc acgaacgcgc ccgagctgct gagcggcgac
    10861 gccaccgtgg cccccgtcgt cgcatacctc agagaccaca aggcggagac gctcgcacgg
    10921 gtcgcaaggg tggcgcagga tttcgccgac gagcacggct ccgagcagcg ccagttcctc
    10981 ctcgcggccg gttcgtcagg aatagacgcg gccaccaacg aggtggtcgc cgtggccaac
    11041 taccgcatgc tcgcgctggt ctacgcgtcg gtgatcgtgc tgtgctacat cacgttccgc
    11101 aactggcgcg ccgtgatcgt cgcgctggtc ccgctcgcca tcacctcgtt cctctgcgag
    11161 gccctgatgg tggtgctggg catcggtatc aaggtgtctt cgttgccggt gacggcgctc
    11221 ggcgtgggct gcggcgtcga ctacgcgctc tacctcgtca cggtgcagct cgccgcgcag
    11281 cgcagcggcc gttcgttggg cgatgcctac cgcgatgcgc tcaatttcac cgggcgggtg
    11341 gtctgcctga taggcatcac gatgggcgcg agcgtgatca cctgggcgtg gtccccgatc
    11401 aagttccagg ccgacatggg catcctgctc gcgttcatgt tcgtctggaa catggtcggc
    11461 gcgctgctgc tgatccccgc gctgtcgcat ttcctgttgc gcacgcagcg cgacgcgcgc
    11521 tagatcgcgt ggtgcgccgg gtgtgacgcc acaccgccaa cccggaccgc gcggccctct
    11581 tcgccacagg acgcccagca ggctggcgac gccgccggcg gcgagtgccg tgcgcagcgc
    11641 agcgggatcg gcaccggatc gccggtggct gaaccgcggc aatcagatgg gccaatcggc
    11701 cgcatcgaca acatggcgcg gctgcgccgg gcgcaagccc ccccaggcgc gggtgaactc
    11761 gccgtccggg tcgaaggtcg ccgcctgctt cgccaggttg aaatgccggc cgccgcgcgg
    11821 gtcgcaaccg acgccagcga tgtattgcca attgccgtaa ttgctcgcga cgtcgtaatc
    11881 gatgagatgc ttctcgaaga aggccgcgcc gtagcgccag tcgagcccga gttcgttcac
    11941 gagacagctc gccgcgatct gccggccgcg gttgctcatc cagccggttt gtaccagctg
    12001 gcgcatcaac gcgttcacca gcggatgatc ggtgtcgccc gcgcaccagc gcgcgaaggc
    12061 gcgcggctcg aagcagcaca gcgcgcgtcg cccgccgatg ccgcccggcg cgaacaggcg
    12121 cacgccatcg acgcgggcgc gccagtgaaa gaactcgcgc cacagcagtt cctgccagaa
    12181 ccagcgcgtg gactcgttgc gcgtctcctc gcgctcgaag cggaacagtt ccagcgcgac
    12241 ctggcgtacc gacaggctgc cgttggcgag ccatggcgac agcctgcccg aggcatggag
    12301 gccgtccagc gcattgcgcg tgtccttgta ccgtgcgacg gcgcgctccc gccacatcca
    12361 gtcgtgcagg cgttgctcgc cgcccgcggt gccgccgcgc gcggtgaaac cggggtgcgg
    12421 cgaagcgacc gcacgcgaca gggcagcgaa cctcgccccg cgcggcgggg gcggcagcgc
    12481 atccggcacg ggatgcacgg cgttcacttc cagcgcctcc acgcgctcgc gaaacggcgt
    12541 gaactggcgc ggcatctcgc cgggtacgaa gggcagctgc tcctggcgaa acagcgtgtt
    12601 gggcggatgc aactcgaggg gaatcccgac acgctcacgc acccgttcca gctggcgctg
    12661 ctcatcgacg ccgccggtgt gggcgacacc gatgcgcgcg attcggtgct gcccggccag
    12721 ctctggcaac agcagttccg gtgctccgtg caacaccagc agttcctgac cgcgcgctcg
    12781 cagtgcctcg cgcagcgcct gcacgctctc gaggaggaat cgttcacgct gggcgccaag
    12841 accggtcacg ttgcaccacg ggcgcggacg cggccagaag aacacgcaca gcagctcttc
    12901 ggcctgcgcg tgctccagca gcccggggtt gtcctgcaga cgcaggtcat cgcggaacca
    12961 gtatatgtct ctcatgtcac ccaggaagcc ggtcgccggc atgaactccc agccgccact
    13021 gccaccatcg agctatcggc cgcgcggtca ggcttccttg tccagcatcc acacgcacgc
    13081 aggcgccgtg ccggcgcgca ggcgtgaatt cctttccagc gactgattct ccgcctgcct
    13141 gaaagttgca accgtcagcg ccacgccacc aaggagcagc aggtgcgcca tccacaccat
    13201 gctgaaatag aagatcgaga gaaatgacag cgaaaaaacc atgctccaca tccagccaag
    13261 cacctgcatg atgaaatgct gggatgccgg atccaggtgt cgcagggggt tgtggcgatt
    13321 gtccatcacg gcatcccagc tgcggataat gagcttcttc atgaccgtat ccccgatgtg
    13381 attgtcactc gagctacggg gtcggttgcc ggatggatca ccgtggcgtg gctggccacg
    13441 gtgggcaggc gacatcgcgc ggacgctccg cggtcgccga agcaatcagc ctgacgatgt
    13501 ggggttcggc gttgcggcga ttgtccggtg cgtactgatc cgcgagttgg agcgcggtcg
    13561 tacttcgtcc ggtcaacaac atgctggagt ccgcaaatgg tccgtaccgc attcgccctc
    13621 gctctcgccg gcgcagccac gttcgctcgg gctgactgcc ccgcgctcct cgatcacgag
    13681 gtgcgcgtgc tacgcaagtc cgacatcgta aatctctgcg atcggtatgc cggccagcct
    13741 ctgctcgtcg tgaacaccgc gagtcattgc ggctatgcgc cgcagctcca gggactggaa
    13801 acactctacc aggagtaccg ggcgcgtggt ctgcgcgtgg ccggcttccc gagcaacgat
    13861 tttggacagg aggcccgcaa ggaagaggat acggccagga tctgttacgt gaactatggc
    13921 gtcacgttcg acatgtactc cgaaacgcac gtgaaggggg ctcgggcaca tccgctgttt
    13981 gcagagctgg cgcgccagag cagagcgccg cgctggaact tcaacaaata cctcgtggac
    14041 ggcgacggca aggttgtagc agccttcggc tcgggcacca gccccgacag ccccgaattg
    14101 cgcgaggccg tcgagaagtt gctctagccg gccgttgcag tcgttgatgc ccacgatcgc
    14161 cgcccccgct gcgatgcgcg cgccgtctgg agcacccttt gctcgcttcc ccccagcggc
    14221 tacaatcgcg cgccttgccc gcatacccgg aagtcggcaa ccatgaacct tcgcgaccac
    14281 ctgaacgctg ccgtcgaagc ggccatgcgc gccgtcggca ttcccgcgga ctgccaggcg
    14341 ctgctgaccc agagcacgcg ccccgagttc ggcgactacc aggccaacgg cgcgatgggc
    14401 gcggcgaagc gcttgggaac gaagccgcgc gagctcgccg agcgcatcgt cgcggccttt
    14461 gacggcgccg gcatggtcga gcggctcgag atcgccggcc ccggcttcat caacatccac
    14521 ctcgcgccag cattcctcgc cgcgcgcgtg gaggctgccg cgaccgatgc gcggctgggc
    14581 atcccggcca gcgaacagcc gcagaccgtg gtggtggatt actccgcgcc caacctcgcc
    14641 aaggaaatgc acgtcgggca cctgcgcagc acgatcatcg gggatgccgt ggtgcgcgcg
    14701 ctcgaattcc gcggcgagcg cgtgatccgc cagaaccacg tcggcgactg gggcacgcag
    14761 ttcggcatgc tgatcgccca cctcgaggac gtgatggcca cgggccagtc gatcgacgat
    14821 gccgcgctcg ccgacctcga gggcttctac cgcagcgcca agcagtgctt cgatgccgac
    14881 cccgccttcg ccgaccgcgc gcgcgactac gtggtgcgac tgcaatcggg cgatgcgcac
    14941 tgccggcgcc tgtggcagcg cttcatcgac atctcggtgg cgcacagcga ggagatctac
    15001 cgcaagctga acgtcacgct cacgcacgcc gacatcaagg ccgagagcgc ctacaacgac
    15061 gacctgccgg tggtgatcgc ggagctcgac cagcgcggcc tgctcgccga gagcgagggc
    15121 gcgcgcgtgg tattcctcga ggagatgcgc gaccgcgacg gcaacgcgat ggcggtgatc
    15181 gtgcagaagg gcgacggcgg tttcctctac gccaccaccg acctggcggc cgcgcgctac
    15241 cgcgcgcagg tgctgcacgc cgaccgcatc ctgtatttca tcgacgaccg ccagaagctg
    15301 cacatgcagc aggtgttcgc gatcagccgc cgcgccggct tcgtcggcga gagcaccacg
    15361 ctcgagcacc atcccttcgg caagatgctg ggcgaggacg gcaagccgtt caagacgcgc
    15421 tcgggcggca ccgtgaagct ggccgagctg ctcgacgagg cggtggagcg cgccgcgcgc
    15481 ctgctggccg agcgcgaggc ggacctctcg gcgcaggagc gcgcggtcgt ggcgcgccag
    15541 gtgggcatcg gcgcggtcaa gtacgcggac ctgtccaaga accgcaccag cgactacgtg
    15601 ttcagctggg agcacatgct gagtttcgag ggcaataccg cgccctacct gcagtacgcc
    15661 tgcacgcgcg tgcgcagcat cttccgcaag gcgggcatcg atacggcggc ccttaccggg
    15721 ccgatcggaa tcgacgcacc cgaggagcga gcgctggcgc tgcgcctggc gcagttcagc
    15781 gaggcgctga gctcggtcac ccatgacgcc gcgccgcacc tgctgtgcgc ctacctctac
    15841 gacctcgccg ggctgttcat gcgcttctac gagaactgcc cgatcctgcg cgacgacgtg
    15901 ccgcccgcgg tgcgcgcgag ccggctgcgc ctggccgacg tggtcgcgcg cacgctggcc
    15961 accggcctcg aactgctcgg catcgagacc cccacgcgca tgtaggtcgc cattcatggc
    16021 gacacgcccg caggtcgcca ttcatggcga caaccgcaga tccgggtcgg cattcatgcc
    16081 gacctgctgg catactggag caggacacgc agcaaaggac tcaggggcat gatgaacttg
    16141 ccggacctgg ccgaggacga actgcgccgc cgcatccgcg cgctcgcccg ggccgacgag
    16201 ggtgaacgtg tcgccgcgct gctcgcagaa gccgacgcgg ctctcggcga cggtgacgcg
    16261 atcacggcaa cggcgaccgg gctgatcgag gccatccgcg gaaagcagcg ctaccgtgcc
    16321 ggcatcaatg ccttgctcgc cgaatactcc ctgtcgagcc gcgagggcgt ggtgctgatg
    16381 tgcctggccg aggcgctgct gcgcgtcccc gaccggctca ccgccgaccg cctgatccgc
    16441 gacaagctcg gcggcggcga ctggcgcgcg cacgtgggca acagccagtc gctgttcgtg
    16501 aacgcctcgg cctggggcct gctgctgacc gggaaggtcg tgcagctgcg cgagcaggat
    16561 gccggggccg cctggcgcat gctgggccat accgtgggcc gcgcgggcga accggcgata
    16621 cgcgccgcga tgcggctcgc gatgaggatc atgggcacgc agttcgtgct ggacaccgac
    16681 attgccgcgg cgctggacaa ggcgcgcgag tgggaagacc gggggtacct ctattcctac
    16741 gacatgctcg gcgagggcgc gcgtaccagc gccgatgcac acgagcattt cctggcctac
    16801 cgcggggcga tcgacgccat cggccggcgc gccggtgccg ccgcgccggc cgcggccccc
    16861 ggcgtgtcgg tgaagctttc cgcgctgcac ccgcgcctgg aactcgcgca gaccgaacgc
    16921 gtgcgcgccg aactggtgcc gcgtgtgctg gaactggccg tggccgcgcg cgcgcacaac
    16981 atcggcctga ccatcgacgc cgaggaagcc gaacggctcg acatcacgct cgaggtcttc
    17041 gaggccgtct atcgcgaccc gcagctcacg ggttgggagg gcctcggcct ggtggtgcag
    17101 tcctacctga aggccgcgcc cgcggtgatc gactggctcg cggcgctcgc cgcgagccac
    17161 ggacggcgca tcatggtgcg cctggtgaag ggcgcctact gggacgccga gatcaagcac
    17221 gcgcagacga tcgggctcgc ttgctacccg gtgtacacgc gcaagagctc cacggacctc
    17281 gcttacctgg tctgtgccgc gcgcctgctc gcacggcgcg agcggctcta tccgcagttc
    17341 gccacccaca atgcgcattc cgcggctgcg gtgctcgcgc tggccgggtc ccccgggggc
    17401 ttcgagttcc agcgcttgca cggcatgggc gaggaactgt acgaggaact ggtggagcgg
    17461 cgcggtgtgc cggtgccggt gcgcatctac gcaccggtcg gggtgcatcg cgaactgctc
    17521 gcctacctgg tgcgccggct gctcgagaac ggcgccaaca gctccttcgt gcacaacctg
    17581 gccgacgatg cactggccag caccacgctg gccgcggatc cgcgcgcaag gctgcgcggc
    17641 ctctcccact atgccaaccg gggcatcgcg ctgcccccgg cgctgttcgg cgcgacgcgt
    17701 gccaacgcac gcggcgtgga cctgcgcgac tacgaggcgc tgcacggctt cgcggccggc
    17761 atggatgcct ggcgcgcgag cgcggcgcga ctgggctgcg cgcacggccc gggctgcatc
    17821 cccgtggtca atcccgccga ccaccgcgag gtgctgggcc actacctccc gagcagcgag
    17881 cacgacatcg agcaggcagc ggcgcgcgcg cacgcggtgg ccgcgggctg ggccgcgaca
    17941 ccgttcgccg agcgctgcgg ttacctgcgc cgagccgccg gcctgatcga ggatgcgctg
    18001 cccgagctgg cggccttctg catgaaggaa gcgggcaaga cgctcggcga cgcgctggcc
    18061 gaggtgcgcg aggccatcga tttctgccgg tattacgccg cgcaggcgca gacgctgctc
    18121 ggcgcgcacg gccacgcact cgcagcgcgc ggcccgatcc tctgcatcag cccgtggaat
    18181 ttcccggtcg cgatcttcac cggccagctg gccgcggcac tggttaccgg caatcccgtg
    18241 atcgccaagc ccgccgagca gaccacgctg gtggcgctgc gcgtggcgca gttgttccac
    18301 gaggccggcg tgccacgcga ggtgctgcaa ttgctgtgcg gccccggtga gcccatcggc
    18361 gcgcgactgc tgccggatgc gcgcatcgcc ggcgtgatgt tcaccggctc caccgcggtg
    18421 gcgcgccata tcgcgctaca gctcgcggcg cgtggcggcg cgcgcgcgat gctgatcgcc
    18481 gagaccggcg gccagaacgc catggtcgtc gactccaccg cgctgcccga acaggtggtg
    18541 gacgacgtga tcgcctcggg cttcaacagc gccggacagc gctgctcggc gctgcgcgtg
    18601 ctctacgtgc agcacgagat cgccgaccgg ctgatcaaca tgctgatcgg tgcgatgagc
    18661 gaactgcgca tcggcgaccc cgcgcacgcc gcgaccgata tcggcccgat catcgaccac
    18721 cgcgcgcgcg cgcgcctgca ggcgcacgtc gagcggctcg ccgggcgcgc gcgcgtgctg
    18781 cacgccggca agctgtcggc cgattgcgac gagggcagct tcttcgcgcc gcacctgttc
    18841 gagatcgatt cgatcgagca gctgccgcgc gaggtcttcg gcccggtggt gcatctgatc
    18901 cgctaccgcg ccgccgacct cgagcacctg ccgcggcgca tcaacgccgc gggctacggg
    18961 ctcaccttcg gcatccacag ccgcatccag ggcagcatcg acctgctggc gcgcgagatc
    19021 gccgccggca acgtctacgt caaccgcaac atgatcggcg ccgtggtcgg ggtgcagccc
    19081 ttcggcggcc ggggcctgtc ggggaccgga cccaaggccg gcggaccctt gtacctggga
    19141 cgcctgctgc gtgccgttgc cgcgcagccg ccggcctatc gccccctgcc cgaggagacc
    19201 gcgggcgacc tgccgcaggc cttcgattgc cgcgccgcgg tgcacgcgct cgccgaactg
    19261 cagcaggagt ggcgccacag cgatctgcac gcgcgcctgg cgccggcgcg cgcgctgcac
    19321 gagcgcatcg cgcacgatcc ctcgcgcgaa cacgatgcgc gcggcgaact tgccgcgcaa
    19381 ctgcagcagc tgctgcagca ggccggggcg atcgggcagc cacgcgccct gcccggcccg
    19441 accggcgagc gcaacacgct gcgcctggac gcccgcggcg tgctgctctg cgtctgccgc
    19501 gaaggcgccg gcgagcgcga cgcactgctc cctgcgctgg cggcactgct ggccggcaat
    19561 gccgtgctgt tcctgaccga cgccgccacc ggcgcgccgg tgctggcggt gcgcaacctg
    19621 ctcgcggaag ccggtttcgc cgacgggctc accgcccacc acgcactgcc cgacgttgcc
    19681 gccatgcagg cggtgatcac cgcctgcgcg atcgacggcg tgctttgtca tggcgacccg
    19741 gcgctcgcgc aacggctgag ccacgacctg gccgctcgcg ccggggcgtt gctgccgctg
    19801 atcgacgacg acttcggacc gctctacctg gagcgcttcg tgcaggagaa gacgatctcg
    19861 gtgaatatca cggccgcggg cggcaacgcc gcgctgatgt cgcaggcgga gagcgattga
    19921 tgatgttcga taccgaaggc gtggtgctcg ccgcgctgat cttcgttgcc gcgatgctgt
    19981 acgcctcggt gggacacgcc ggcgcctcgg gctacctcgc ggcgatggcg ctgttcggcg
    20041 tggcgccgga ggtgatgaag cccaccgcgc tctcgctgaa catcctggtc gcgctgatcg
    20101 cgaccttcaa gttctaccgc gccggggcct tctcgtggcg gatcttcctg ccgctcgcgg
    20161 cgggctccgt gccgctggcg tggctgggcg gcacgctggt gctgccgggg catttctaca
    20221 agccgctggt cggcatggtg ctggtctacg cggcctgggc ctcgctcaag cgcgcgagcc
    20281 ggccgcagga cgcgcaactg cgcacgccgc cgaccgtgct gctgattgcc tgcggcgccg
    20341 tgctcggcct gttgtcaggg ctcaccggcg tcggcggcgg catcttcctc agcccgctgc
    20401 tgttgttcct gcgctgggcc gaggtgcgcg tgatctcggg aatcgcagcc gccttcatcc
    20461 tgctcaactc gatcgcggga ttgctgggcg tgatccgcgt ggcgcccgaa ttgcccgccg
    20521 cgctgccgct gtgggccgtg gcggcgatcg ccggcggctt cctcggggcc gagctcggca
    20581 gccggcgcct gggcaacgcg ggcatccagc gcgcactggc actggtgctc gcggtggccg
    20641 gcgtgaagat gatcgcgacg gcctgagcgc ccggggctag acgaaacgca ggcccgcctc
    20701 gctgaacggc agcttgcgca cgcgcgtgcc ggtcagcctg gccacggcgt tggcgagcgc
    20761 cggcgccacc agcggcgtgc cgacctcgcc catgccgccg ggcgcatcgc cgctggcgac
    20821 gatcgcaacc tcgatctgcg gcgtctccga catgcgcagc ggctggaact ggtggaagtt
    20881 ctgctcctgc accaccccgt cgacgatgct gatgcggttg cgcaacgcgc cggtgagccc
    20941 cgtgatcacc gccgactcga tctgcgcgac cacctggtcg ggccgcaccg cgatgcccgg
    21001 atcgaccgcc accgcgatat gcgcgcagtg cacgtccttg ccgcgacgct ggacctcggc
    21061 ggtcatcgcg atgtagctgc cgtagcactc catcgacgca atgccgagcg ccgcatcggc
    21121 gccgcgtgaa cgctcccatt gcgccatcgc ggcgaccttc tccagcaccg cgcgctggcg
    21181 tggctgctcc gccagcatct cgaggcggaa ctcgagcgga tcacgcttgc cggacctggc
    21241 cagctcgtcg atgaagctct cgaaggcaaa ggcattcagc gcatggctga ccgagcgcca
    21301 gtagcccacg cgcacgccgg tgtccctgat caccacgcgc gagcgcaggt tcggcaccgc
    21361 gtagaccagg ttgctcgcgc cctcggtcat gaacggatcg agaccgtcct tcaccacgcc
    21421 gggaaaggcg cgctcggtca ctgactgcga gaccatcagg tgatcgagcg cgatcagctt
    21481 gcccttggca tcgagcccgc cgcgcagccg gtgcaggctg gccgggcggt agaagtcgtg
    21541 cgtggtgtcg tcctcgcgcg tccagaccag cttgaccggc acgcccggca cggccttcgc
    21601 gatcgcggcg gcctgcgcga tgaaatccac gtcgaggcgg cgcccgaaac cgccgccgat
    21661 gaactgcgaa tggatcctca cccgttccgg cgcaagcccg gtgcgcgcgg cggccacggc
    21721 ccgggctccg gccgggtact gcgtcgaggt ccagatgtcg caaccctcat cgtccacgcg
    21781 ggcgaggcag ttcatcggct ccatggcaac gtgcgccagc agcggcagct cgtaatcggc
    21841 ctcgaggcgt ttcgtcgcgc gcgccagccc ggcatcgata tcgccattgg ccctggccac
    21901 cgcgccggtc tccttgaggc ccgcgcgcag cgccgcggcg acggaggcat cggacaccgc
    21961 cgtgcccgcc cccaggttcc agctgatctt cagcgcatcg cgcgcctgtt tcgcgatcca
    22021 gaagtgatcg gccaccacgg ccactccctc gccgatgtcg atcacgtcgc gcacgcccgg
    22081 catcgcccgc gccgccgccg catccacgct cgcgacggtg ccgccgatca ccgggcactg
    22141 ggtgagcgcg gcatgcacca tgcccggctg cgtcgcatcc agcccgaaca ggcgctcgcc
    22201 gtgcaccttg gcgcgcgtat ccagccgctt ctgcgtcgcc gatccgatct gcgtgaacgc
    22261 gcccggcgcc ttcaacgcca ggtattcggg ggccggcacc ggcaggtctg ccgcgatgcc
    22321 cgacaggaag ccgtagctga gcttgttgcc gttgcggttg tgcacgtagc cgtcgcgggc
    22381 ctcgagcacg cccacatcca cctgccacgt cctggccgcg gcttcgagca gcaccgtgcg
    22441 caccgtggcg ccggcgcggc gcagcggcag ccaggcatcg cggatggagg tcgagccgcc
    22501 ggtgatctgc gcgccgatca tcgcgttcgt gtacaccgcg gccaccggcg cctgctcgat
    22561 gcccaggctg gcaaccggca cctgcagctc ttcggccacc agcatcgcca gcgaggtgtg
    22621 cacgtcctgg cccatctcgt tgcggtgaca gatgaaggtg atgcggttgt cggggtcgat
    22681 gcggatccag gcgttcatct gtaccggctc ctcggccacg cccgcggcgc ggctttgcgg
    22741 caggtgcagg ccgaggatca tgccgccggt cgcggtggcg cccagcttga ggaattggcg
    22801 acgttccatg gctcagccct ccagcgccgt ggcggcgttc ctgatggcct ggcggatgcg
    22861 cgaataggtg ccgcagcggc agatgttgcc gtccatcgcg gcggtgatcg cggcgtcatc
    22921 cggcctggga ttgtgcgcga gcagcgcggt tgccgacatg atctgccccg actggcaata
    22981 accgcactgc ggcacgtcga gggcttccca ggcctgctgc actgcctggg cgaccttgcc
    23041 gtcgagcccc tcgatggtgg tgatcgcctt gccggcggca tccgccgcgc gggtcgcgca
    23101 ggcgcgcgcc ggctgaccgt cgatgtgcac ggtgcaggcg ccgcacagcg ccacgccgca
    23161 gccgaacttc gtgccgggca tgtccagatg atcgcgcagg atccacagca ggggcgtatc
    23221 gggcgcggca tcgacacggt gctcgcggcc gttgatcgtg agctggagca tgggcgttct
    23281 cccgtcctgt tattggcccg ccgattatgc acccggcgct agaacacgcg gtaacgcttg
    23341 cgcacgtggc gctggcgcat ctcgatctcg cgccggcgct gatccgggcc caccgtcggc
    23401 gtggacgggt gcaggtgcgc gcgaatttcg tcgaaacgca ccttcgtcgc cgtgatctcg
    23461 ggccactgat cctggggcgg ctgctcgctg taggcccagc gcggcgacag gaagccctgc
    23521 gcgtggcctg cggtgtcgag ccagttcggc acgccggggt cgcggtgcgc gaccaccagg
    23581 cggatcacgc catcgggatc cacgtggctc tgccagccgt tcagcgaggt gatctggctg
    23641 gcatactcca atgattcgcc ccacaggttg ccgagctgga agccgatgta gcgcggcgcc
    23701 accttgatgc ggctctcgat caccagtgcc tcgtcgggac cgaggtcgaa cacgccgccg
    23761 gcatagaggt tggtgctcat gccgccgccg gtcgcgcccg aggccgcgtt gatggtattg
    23821 aacgcgttgc gcggcatcgc gatacccggc atcgagccct tgcgctcgcc gtagacgccc
    23881 atcgggatgg tccagaaggc gttccagaaa tgcatctggc ccctcaccag ctggccgaag
    23941 cgacggatgt cggcggcggc ccggaccggg gtgtagggct ccggatgcgt gccctcggca
    24001 ccgagctgcg tcatctcgaa atggatcgca tcctcgcgcg cccagtcgta gaacagctgg
    24061 cgaccggaaa tgtagctggc atagcgctcg ggcggcatag tgggatccgc cgggtgcggc
    24121 cggtgctcca ccttcagcgt gcagatgtaa ttgccggcaa agcccgcggg acgctcgggc
    24181 gcgaacagga tctcgaagct gccgtcgggg ttcacctcga tgcgcgagca gtcgagccgg
    24241 ccggtctgca gcttcatgcc gggacgcagt tccgagagat cgccggtgtc gccggaaaga
    24301 ccgccggtgc tggcctcgaa gatcaggtaa tgcggcgcct tgcgcccggt cggcgccgcc
    24361 gcctcgccct tccagtgccg cgcatcgccc atctccccgc gcagcaggta gctcttgctg
    24421 ccgtcgatgg gcgcgtagaa gtagatcgcg tccgggttgt cgatggtact gcggttgaag
    24481 atgctgagcg cgttgtggaa atgcgggcgc accgggtcct cgtggaaagc gcgctcgata
    24541 ccgtgatgca cgaagcccat caggtagcga taaccctcgg cgagattgcg ctctgtgggc
    24601 ggcgcgggca tcagttgcgg gacgtcgatg ccgtcgcggg cctgctgcag cgactcgatc
    24661 atctcgtccc atgccgcgcg cagctggcgc ttcgcatcat tgctcgtttc gctcatcacc
    24721 actccccgtg catgtcgtta ttgccaggcg ccgagtatcg atccccccgc gcacccatgg
    24781 caacccggta tcgcgtccgc ggaccgcgaa ggacccggcg ccgtcggcag cgctgcctgc
    24841 gccgggaggc agtccccgta gtcctgcccg cgccgcgata cgatcgcacc cggtgcgatg
    24901 aaagcctcgt gcaggcgcgc gctcacggct gccagtccac cgcgtgatcc gcgagatagt
    24961 cgtcgacggc cgcaccgagg gtcgggtgga agacgtcagg gccgaagtgc tccatcagct
    25021 cgaagcgcct cagcttgtcc ttgaccggat ccttcatctc ggcaaagtgc agctcgatgc
    25081 ccgattcgcg cagcgtctgc tccatttcgg ccagtacatc cgccgaagtc acgtccacgc
    25141 tggtgacggg ctccgcggaa acgatgatgc gtcgcaccgg cgtgggcgaa tcggccacgg
    25201 cctgcaggac tcgctcccgg aacagttcgg cgttggcaaa gaacagcggt gcgtcccagc
    25261 ggaacaggac cagtcccggt acccgtcgtg cccgtggata gcgcttgatg tcgtggtagc
    25321 cacggatgcc gtccacgcgc cccatcaccg cgaagtgcgg acgccagccg tcccacagga
    25381 attcgatgat cgcggcaacc accgccaggc cgatgccggg aatcgcgccg agtaccgcca
    25441 cgccgacgaa gcagccgatg gacagccaga attcccagcg ctggatgcgg aagatgcgcc
    25501 gcaggtcggc gaactcgaac agcccgatgg ccgaggcgat cacaacggcg gccagcgcgc
    25561 tggtgggcag attgcgcagc aggtcgggcg caaacaccag cagcagcgcg accgcgagcg
    25621 caccgaccac gccggtgagc tgggtcttcg caccggcggc ttccgccacc ggcgtgcgcg
    25681 aggagctgct gctgatcgga aagccctgga aaaacccggc agcgaggttg gccgcgccga
    25741 ggccgatcat ctcctggttc ggatcgacgt agctgcgcgt cctggcagca taggtgcgcg
    25801 acagcacgct ggtgtcggcg aaggacacca gcgccacggc gcagccgccg atcacgacgg
    25861 cgccgagatc ggccagcccg acccacggca gcgcaaacgc tggcaggccc tgcggcagcg
    25921 caccgagcac cttcacgccc gccgtctgcc cgagatcgaa caggccgacc gcgagcgtcg
    25981 cgcccgccac cgcgatcagg atgcccggga tgcgctgcca gggcttgagc aggaatatca
    26041 gcgccagcgt ggcggcgccg atggcgaacg ccgtccagtt ggccgcgcca cccagcagcg
    26101 cctcgccgat atgccacagg tcgcgcagcg gaccgtcgct gtcgatcgag atcccgaaca
    26161 gcttcggcag ctggctgatc agcaccgtga acgcgatgcc gttcatgtag ccgtagcgga
    26221 tcggcttgga cagcagctcg gtgatgaaac ccaggcgcaa caggcccgcg agaatgcata
    26281 ccagtcccga caccacggcc atcatgccgg ccagcgcgac ggcccgcatc gggtcgccgc
    26341 ccgagagcgg cagcacgacg gcgaggatca gcgccgccag cgaggaatcc ggtcccagca
    26401 ccaggatgcg gctggggccg aacagcgcgt aggcgagcag cggcacgatg gtggcgtaga
    26461 gcccgtagat gcctggcacg cccgaggcca cggcgtaggc gatgcccacc ggcaccagca
    26521 tcgtggtcag cacgaggccg gccatcaggt cgtgcgccag ccacgcaggc tcgtagcggg
    26581 cgagcatctg cacgcccggc agccagcgct tccagccggc gagctcggag ctcaccgggc
    26641 gcatcagacg acccggctcg ggcggcccaa gggaggaaac atcgctgctc accggcatgt
    26701 gcacgctctg tttgcggtga tgaatgatgg tccacaagtt acaccaggat gcgacgcccc
    26761 gcttaccgat gggacccggc ctggcggtgc gaggcactga agccggaagt agcgggagtg
    26821 ggttatgatc cggtcatgaa tcgcgcaacg gagatcctcc atgaggcacg ccttcaaagc
    26881 cttcctgctg ttggcattgg ccacgacgat ggcaccgcag ttcgccagcg ccgggcccga
    26941 ggccgcaccg cgcgtcggcc tggttctggc gggcggcggg gcgcgtggcc tcgcgcacat
    27001 cggcgtcatc aagtacctgg aagagaacaa catcaaggtc catgccatcg ccggaaccag
    27061 catgggttcg gtggtcggcg gcctgtacgc ctcgggcatg aacgccgcgc aggtcgagga
    27121 ggtcgcgcgt accctcgact ggcgcttcgc cttcaacgac agcacggccc gcgagcagca
    27181 gagcttccgg cgcaagcagg aggatttcga cttcctgatc ggcgccaggc tgcgcttcaa
    27241 ggacggcagg ctcagcattc cgatgggcgc aatcgagggt cagcacctga acctgctgct
    27301 ccacgacctg gtgcggcacg tctcgggaat cgacgacttc gacaagctgc cgatccccta
    27361 ccgggccgtg gcggccgacg tggtcaccgg cgacgcggtg gtgctggatc gcggggatct
    27421 cgcggtcgcg atgcgcgcca gcatgtccat accgggcgtg ttcgcaccga tcgatctcga
    27481 cgggcatctg ctggtcgacg gcggcatcgc caagaacatc ccggtcgacg tggtgcaggg
    27541 catgggggtg gatcgcctga tcgtgatcga catcggcacg ccgctggcac agcgcgagga
    27601 actcgggaac gtgctgtcgc tgatcggcca gctcaccacg atcatgacgc gcaagaactc
    27661 cgagcagcag ctcgccctga tgcgcgaggg cgacgtactg atcctgccgg cgctggatga
    27721 agcgggcgtc cagacaatga gtttcgacag ggtcgacgac gcgatccggc tcggctacga
    27781 agcggccagc gcgctggccc cgcagctcgc cgccttcgcc gcgccgggcc cggcgcaaca
    27841 ggtggcgggc atcacggagc ctgggccgcc gcgcatcgac gcgacgcgcg tggagaccga
    27901 cgcgaacgtg tcgaagcagc tgttgcgcag ccgcgtcacg caaccgctcg gcgagccgct
    27961 cgatcgcgag ctgctcgagc gcgacatcgc cgagatctac ggcttcgaga ccttcagccg
    28021 cgtggattac caggtgcagc caaccgcggc cggcaacgag ctcgtcgtgc gcgcgacggc
    28081 caacccgggc ggtgtgaact acctgaagct gggcatgagc tgggaccagg acagccatgg
    28141 gaccagcgag ttcggcctgc gcgcgagctg gcgccagcgc ggcatcaatg acctcggcgc
    28201 cgaatggttc acctatggcc aggtcggcgg caactccaac ttcggcaccg agttctacca
    28261 gccgctggat cagctgcagc actacttcgt cgagggccgt taccgcttcg accagcgcag
    28321 gctcaacctg agccgcaatg cccgtgtgct cgcgcgcgcg ctcgtcgacg ggcaccaagt
    28381 ggacctggcg ccgggaatca acctcggcaa ggtcgcgcag tggcgtgccg gcgtttacgc
    28441 cggcacggcc aacacggacg tcgagatcgg agcgccggaa ctgtcatcgc gcagcgacaa
    28501 cgacggaggc tacttcacgg aactgggcta cgactcgctg gaccggccct acttcccggg
    28561 cagcggactg cggctgctca gccgctactc ctggagcgac gggagcctgg gggccagcga
    28621 ggactacgag gcctggaaca cgtccgcgtt cggcagcatc agcctggggc gcaactcctt
    28681 caccgcaatc gggcgttggg cggaactcga tctcgatggc agcggagact ccctgaccgg
    28741 ctcgaacgtg ttcctctcgc gcgcatacac gctgggcggc ttcctcgcgc tgtcgggcta
    28801 caccagcgat tcgctggcgg ggaactacct cggcttcgcg ggcctcacct attaccgccg
    28861 cctcaccgag cagtcgctgc tgccggtaga catgccggta tacgcgggtg cgagcctcga
    28921 ggcaggcaac acctggctcg atgaatcgga cgcgagcttc gacgacctga tctacgcggg
    28981 cagcctgttc ctcggtgtcg actcgccggt gggtccgatc tatctcggcg tcgggatggc
    29041 cgagaacagc cagtacgcga tgttcctgaa aatcgggcag atattcgact gacacggcgc
    29101 ccatgggtcc gcattcatgc ggagatcaca ccgcgggtcc ggcttaagcc ggacccgcgg
    29161 gccgatgcgc gcaggggccc cggcatcagc ccgggttgaa tcccatcacc gcct
//
LOCUS       DAORUQ010000020        29177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1156_length_29177_cov_6.784287, whole genome shotgun sequence.
ACCESSION   DAORUQ010000020 DAORUQ010000000
VERSION     DAORUQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29177
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_29177_cov_6.784287"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLW13_03330"
     CDS             complement(<1..1010)
                     /locus_tag="PLW13_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69431.1"
                     /translation="MITENADKFRPVAPPRIVEDAYTEDQHRRLLDVVRNNGPWQLIL
                     AENFKTPEEVIATTSGMLPEGVELTWDMILNPVFRGYLARGGACFYPELEDCYYNSRF
                     LALVREYWGAKYAEPETFLFNIQGPSPIGGPPHLDGTVFRGMTMENTPLWLLLTMAKS
                     CLFRHWQVKKGQVIAWYYKGRIGGGFNCWPDGPRGEPLQINAPLWGRAVVVENEMMFH
                     HGQACGPLAMRKPAGLDISSRFGADPEVEGGWRITTGDVVNQKIPAEEMRFLVHWGAR
                     LYKDFDEMKVYMDHSDDISAEQALNILIDDMRKRGVAFEMPTDPMNDRSFIGLVSQVY
                     DIG"
     gene            complement(1051..3021)
                     /locus_tag="PLW13_03335"
     CDS             complement(1051..3021)
                     /locus_tag="PLW13_03335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY69432.1"
                     /translation="MIAEPTNTGSMQDAAIDALDPVIALLALVQSTGDVSLLRKYGPA
                     LEGTQHQVKEAFVAFDTPREPSAASLEVAREVRDLLRAAVRSGRPAVLATLDKPLFRE
                     MARLALGLEMPEMSIDVAYQHGGFTTDTRVRQPKRLPPADFKVLVVGAGMMGINAAVK
                     LQQAGFDFQVIEKLEAVGGNWLENTYPGAAVDTPSRVYSFSFEPNASWSKYYPVGPEF
                     LAYLERVTDRYKLRERITFNTTVEGAQWDEERKLWKVNALQDGNKVVFEGQALIMAVG
                     PNNAPNYPDVAHLDTFAGPVIHSAAWDHSVDLQGKKVVLVGTGCSGVQVANAIADTVG
                     ELVIIQRQPEHIIPNPAAHDPVDELERWAMEHIPFVVQWKRLQSLSSAMQDMHGMVMK
                     DEEYAAKTGGFGPINDGIRMMCEAYLKSHFPDDPGMVALLTPSFPVFAKRPILDCGFY
                     DTLKKRNVSIVRGALEACDEKAVILADGTRIECDVLLLSTGYKLFFGRQFDIRGSGGR
                     TLKQTFEPYPFSYEGMLIPGFPNFVFMGAPYSYLVANHAVVSEQQVHYAIELLQWMVD
                     DGLASVDVTESAARAFVEDVDANLARTAWVQCGNAHGYYRDHSRKVILAIPRHNSRIW
                     HDTRSPRTGDFTVTRRPEMGPAVEPEMAMLTI"
     gene            3284..4090
                     /locus_tag="PLW13_03340"
     CDS             3284..4090
                     /locus_tag="PLW13_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69433.1"
                     /translation="MSEGNIIEVSDLHFAYGDRAILKGMDLHIPRGKLVAILGTSGCG
                     KSTLLRLIGGQLRPARGKVLIDGEDVHALDNDGLYRLRRRMGMMFQVSGLFSDLSVFE
                     NVAFPMREHTDMPESMIRDLVLMKLHAVGLRGAHAMMPGELSGGMQRRVALARAIAID
                     PLLIMYDEPFAGLDPISLNVIAGILRHLNDALGTSSVVVTYNVSESLKVVDHAFFIFD
                     GKIVAEGSTADILASDNPFVRQFVDARPDGPVSFHYPAKPFASELRLGGS"
     gene            complement(4241..5062)
                     /locus_tag="PLW13_03345"
     CDS             complement(4241..5062)
                     /locus_tag="PLW13_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY69434.1"
                     /translation="MDNVRIEYPRPEVAVLRLNRPECLNALSWALVGELHAALDAIHA
                     NNRCRIVVLTGAGKGFCAGLDLRDQGNPGTIVDGLPPGPQAGLIAQNHMASLIPHLRR
                     IQQPLIAAINGVAYGGGLALALGCDIRIAAQSARMCVQFIRLGVSGCDIGVSYMLPRL
                     IGASRAHDLILSARVIDAAEAERYGIVTRVVPDGEIESAALALADELCEFSPFGVFAT
                     KQVMWANLEVPNLEAAIQLENRNQIMAGLSGETEEAARAFFEKRKPRWSQARGEG"
     gene            complement(5110..5880)
                     /locus_tag="PLW13_03350"
     CDS             complement(5110..5880)
                     /locus_tag="PLW13_03350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69435.1"
                     /translation="MDRAGLGKLFDMTGRVVIVTGGTRGIGRALAEGYAAAGAKVVVA
                     SRKPEACAETERHLRAMGAEALGVPTHLGDLDALRVLVQRTVDAFGAIDVVVNNAATA
                     LALPVGEFTLEAWNKSMDVNLRGPVFLVQEALPWLKQSPHAAVLNVLTGAAFLFSSTI
                     SLYAIGKAGMMAATRAMAAEFEPFGIRVNALSPGTVDTDMVRKNSPEAQDVMTSIQLM
                     KRKASADEMVGPALMLTADAGSFITGHVVFADGGMVPR"
     gene            6207..7586
                     /locus_tag="PLW13_03355"
     CDS             6207..7586
                     /locus_tag="PLW13_03355"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY69436.1"
                     /translation="MKKPTASIVAAITLALACAATQAAVDAQAAARLGTTLTPWGAEI
                     AGTPDGSYPAYEGGIKEPPPGYDPKSGMWPDPFPEDKPLFSIDAKNMAQYQDRLMEAQ
                     MELMRRYPDYRLDVYPTRRSAMVPENAARNALANARNPECRTSSDGVGLYGCWGGTPF
                     PIPENGYEAMWNHLLTYTSPGETNSVGYLVNANRATTMLLLSRSIADYPYFNPQQTPY
                     EGAGIYYLRSLSTTLEPAREAGTQTLLWYPLHYDSDEQRAWSYQAGQRRVRLAPEFAY
                     DTPVAQFGGAMFFDEINLFAGRMDRFRFELKGRKLMYMPYNNYRMHLLRGDPAQLLGP
                     HHFHPDKVRYELRRVWVVEATPLPNVRHVAKRKLFYLDEDSWIALAYEGWDQSDRLFR
                     LLLSNTMANYYTGGVIQGASVQAYDLARGQYAGLQLLIAPRCYIRYGLPQQPDAEMAP
                     ARMGARGIR"
     gene            complement(7599..8369)
                     /locus_tag="PLW13_03360"
     CDS             complement(7599..8369)
                     /locus_tag="PLW13_03360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69437.1"
                     /translation="MTDRLKGKAIIVIGSGSGIGAATVRRLAAEGARVCAADINEAGA
                     KAVAEEVVAAGGEAFAVHIDLADEASVNAAVAAAVARLGGLDGAHVNAADLQTIMRDS
                     DALSVDLAVFDRTLAINLRGHLLCTRAVLPQLLARGGGAIVYTSSGSADSGEPVRPSY
                     GTSKSGLNAFMRHVASRWGREGITANCVAPGFTLTPEMQRSGQLSQELLDGMRARIPG
                     TRLGRPEDIAGVVAMLLSEDGRWINGQVYNVNGGALMR"
     gene            complement(8460..9470)
                     /locus_tag="PLW13_03365"
     CDS             complement(8460..9470)
                     /locus_tag="PLW13_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY69438.1"
                     /translation="MELVMIFDMRAPAFGAPRRELYAAALDCAAWADAIGFDVIGFGE
                     HHLAEDGYNPSPLVLASAMAGRTRRIRLRTAVLLASCYDPVRLAEDLAVAQLVSDGRI
                     ELGLGFGYRPVEFAMYGRKLEDRFDFTCRIAQFLGQAFSGEAFEWEGRPGRISPLPDK
                     PVPVLLGGMAPKVARAAARIADGFLVPLFPADVWQPYRDECLKLGNADPGLYPQQGPT
                     FLWVSEEPERDWEWIAPHVLHVLDSYSRWTSAAYGKPIGPYAGGMTAGTVRQSAAYQV
                     LTPAQTIELAQGLGDHSSLYLTPLFGGIEPARAWKMLRLFEREVYPQLPRGPVPRWGL
                     RA"
     gene            complement(9532..10701)
                     /locus_tag="PLW13_03370"
     CDS             complement(9532..10701)
                     /locus_tag="PLW13_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69439.1"
                     /translation="MRIIDVDSHFNEPYGWFEQANPALAAKLPRMTAAEQFIDIVVGD
                     LFASVPPQLRPDPLALVPDYVRESYERFLSGEGEPPPSAVESGSFLPAAHEPEARIAW
                     MDARGIEKQVLLPSSGYHPYRHAMRKDPSLALPALETYNRWAAERLHGYTERLIPATV
                     VDLMDVDWSVAEITRMRAGGSRVAFVKADPHGGKALNHPDFERFWSAAEDLGVTIMFH
                     VGGGRAAMHPGWANNGGDLAAFYRLAALARQLVPQMALGAMIFGGVLERHPKLGILVS
                     ELGIDWLPHFLDTIDLEADNTRPRLLTMAPYELPLKPSEYMQRQVRVSVVHQQDVLRP
                     TLDRIPEGLVVFSSDFPHVEGHQEAVALYDAQLAGLAPGAREAFFGRSAAQLMGI"
     gene            10793..11494
                     /locus_tag="PLW13_03375"
     CDS             10793..11494
                     /locus_tag="PLW13_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQY69440.1"
                     /translation="MSDRISQGSSGARSSSRRGRPTAAEAKHKNERILAAATAAFVAH
                     GMQASIEAIAQVAGVSKPTLYARYPDRLALFEAVLGQLHRNRPSMDTVITAEMPLATA
                     LERYARALLPALTSPEVGAFHRFTLQGSPLERQARRVMIRDFNAAFVSPLVDYLNACA
                     SSGNMGAGIDATTAARMFVHQVIGLIDYHVATRDSRAAAIRGARRELASVIAIFIGGL
                     QPRADLSPAASARPR"
     gene            complement(11463..11870)
                     /locus_tag="PLW13_03380"
     CDS             complement(11463..11870)
                     /locus_tag="PLW13_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY69441.1"
                     /translation="MSTLSNNYSFTKIVVDDLDRTADFYKRVFDYKEFQRVQSDVAGE
                     PMSEIIMVKGTSMEGDLPLVIWKWPRRPAPAASDVILGFVTSSVDELVARVPAAGGRI
                     IQAPKDDPEHGVRVGFVADVDGRLIEVVQMLQG"
     gene            complement(11938..12921)
                     /locus_tag="PLW13_03385"
     CDS             complement(11938..12921)
                     /locus_tag="PLW13_03385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HQY69442.1"
                     /translation="MFAMIINETGGPEVLQYGEIATPEPGPGEVRIRVAYAGVNPADW
                     KNRQGMLAAFRPYVFPYIIGFDAAGVVDKLGAGVSGFNVGDRVFTPTNHGQGGQGSYA
                     QFAIATADRVAHMPQGLSFAQAAALPVAALTAWQGLFDRGELKAGQRALIHGGSGGLG
                     GFAVQFARWAGAHVAATCSTPNVEYLRELGVERVIDYRRENIAEAVATWAPGGLDYLM
                     DAVGVSTLPNGLDLVRAGGTFVSIPTLNDDGDIPAAAAAGAARGVKRVFSTMTDIGCG
                     PTLTKIGELLVAGHVKLPPLREFALREAAAAHRDIQGGHTRGKIVLKVADL"
     gene            complement(12942..13811)
                     /locus_tag="PLW13_03390"
     CDS             complement(12942..13811)
                     /locus_tag="PLW13_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69443.1"
                     /translation="MAKLLEGKIALVTGASRGIGRALAQRLGAEGATVVVSARSLDTA
                     GQYAGTLRETVALIEAAGGRAMPLVADIENPADVEGLVGRAVALAGGLDILIHSGGKA
                     EYAPTAEMPLDMFDRTIQHYLRAPFALVQKAVPEMRKRGAGWIVVLGSVTAQPPGRPY
                     DEWATYGGATVYAAIKAAVHRMTQGLAAELLPDNIAVNTLAPSTAIRTPGAVDLIPEE
                     YPSERIEYIVETGLALCHLPARERTGLTAYSLHFPLAHKLPVHTLDGRERIEDPVIPP
                     YAHPAIVASGEGS"
     gene            complement(13912..14946)
                     /locus_tag="PLW13_03395"
     CDS             complement(13912..14946)
                     /locus_tag="PLW13_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HQY69444.1"
                     /translation="MIVVQDRIDVAPADLPRLRTMLRERYLPGAAARGLTLIDELVSP
                     PLQLQERPNTLWLRWSLADAGAFWAARYASGDPAVVAFWAELDTFVLERERHYLVPAD
                     AALPGPEDTTAYRCQPLAWRETAQLYLKNEITAAERAALVALLERAATELPGLGTVSL
                     AANFVPDYGAGHYTWDLVYPDRATAETARASSFWREQLLPALDRHCRARAALGLETLG
                     AGARAPALASGVKRTALFRLLPDVSPELQARFARDTLEMAAQIPAILNWRLSRAVALA
                     WDAPDCAPWSFVWEQEYATLDGLNVDYMVHPHHWAHIDRWFDPESGAQIIDTALCHAF
                     APLAQSVIVL"
     gene            complement(15130..15984)
                     /locus_tag="PLW13_03400"
     CDS             complement(15130..15984)
                     /locus_tag="PLW13_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69445.1"
                     /translation="MDKERAVFGGGVAVITGAGSGIGEGLARHAAALGMKVVLADIAV
                     ERMEAVAAEIRAAGGEAMAVPTDVTDAAAIDRLAAITHETWGDVRLLMNNAGVETLGF
                     VWEMSAATWDKTLAININAMVHGVRAFAPRMIAAGKPAFITNTSSIGGLGMMPVQTSY
                     VMSKHAVLSFSECLSLEMQVKKAPIQVSAILPGPVATRIFEDSKPGTDPMVAHHREVM
                     RAMLEQSGISGLEAAQRILRQIAAGEFWVSTHPEMTAEFARQRAAHLSGLTKPQMAAE
                     TLALLGAH"
     assembly_gap    16411..16420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16421..17949)
                     /locus_tag="PLW13_03405"
     CDS             complement(<16421..17949)
                     /locus_tag="PLW13_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69446.1"
                     /translation="MADILIRGGTVIDGTGAAAYPADVRVTAGLISEIGRGLAPRAAE
                     EVHDASGCYVTPGFIEAHTHYDATMWWQPDLDPLPGNGATTIILGNCGFAAAPMSRDK
                     AAQLEMVKIFSFFEDIPMGPFLENVPWDWETWSEYKASLTSKVKVPANYAIFVGHIAI
                     RIAVMGLDAWERVATPAETARMAQLLEDALAAGALGLSDNMHDHDGDNRPIPTLVADD
                     AEFTALFDVLERYPAASYQVIVDTAMRKTGPQSLERLERLLKGRRTRVQIAGAIPTLE
                     FQKDILPAMEASVKKMQDAGVDLWPGYAHVSPTSTLSLVKSLIFAQSNDYVWHEVVLA
                     ETHEEKARLLADPGWRARARESWDTKAWKHSPMNNPQDLYLLDSANGTGPVDITLKQF
                     ADSRGLHRSDAMAQWILENGTHSTVHMAPFPKDEALTTRLLLEPKTVGNISDAGAHLQ
                     MLCGGGENILLFSKYVKENRITLEQAIHVMTGKLANFFNLHDRGEIRVGKRADIAVFD
                     L"
     gene            18183..18923
                     /locus_tag="PLW13_03410"
     CDS             18183..18923
                     /locus_tag="PLW13_03410"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY69447.1"
                     /translation="MAGTGRLQGRIAIITGAAQGMGAEHARAFIAEGARVVLTDLQEP
                     AGQALAAELGPNALFVRHDVSKAQDWDAVIAATRTAFGPANVLINNAAMYFICPVDEA
                     DPERVRKLLDVNIYGSWLGISKLAPVMREAGGGSIINLSSLAGMRGIPWHGIYGTSKW
                     AIRGLTKTAAYDLGPSGIRVNAILPGAIEETGMAGGPLDETQLAAIPLRRTGRKSEVS
                     QLAVFLASDESAYITGGDHVIDGGRALW"
     gene            complement(18954..21098)
                     /locus_tag="PLW13_03415"
     CDS             complement(18954..21098)
                     /locus_tag="PLW13_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69448.1"
                     /translation="MSQPAYSHLLSPGRIGAMQLRNRIVVMAMGVNFSEDDGSSGDRV
                     VAYHEEQARGGAGLVISGACGVMYPIGQVQPWQIGISDDRFIPGLARVVEAVHRHGAK
                     YAVQLHQGGLVAGDDTKAGRPQWCPSVPEPMKGDFTEGFLLEELQAFASAGMPTYKVL
                     THEDIQVVVDAYAAGARRAKQAGCDAVEIHGGHGYLLSSFLSPKTNKRTDEYGGSTEN
                     RSRFLVEVVRAVRAAVGPDFAVIVKMDSREVGKEGGITLEHARVTAKLVEQAGADAIT
                     VTAYHDTGIGKLHSASNIPHEPNTNLPAGAEIKKIVDIPVICSGRVEPEHGDAEIAAG
                     RLDFLGMGRKLLADPGLPNKLAAGKAEQVRPCIYCYTCVSAIYTLESTRCAVNAECGI
                     EYLRVDAPKPGARKRVVVVGGGPGGMESARRLARDGHEVILIEKGDRLGGTLRFASLA
                     YAANERLLDWLRLQVEQATIEVRLNTVATPELLKSLKPDEVIVATGALRNMPPIPGAD
                     LAHVFSGDDMRKMMLGESSEALAQKTSLLTRIATKAGAATGLTANLDFVRKATHAWMP
                     LGRNVAIIGGELVGLELAEFLIERGRKVSVIDEVPRLGKGLTLVRRMRLLAELKEHGL
                     GLFASAKDIRIETGRVSFTDAGGAARTVEADHVIVAKGATGDSSQAEAFRAAGLRVHE
                     VGDGTGVGYIEGAMRGAMLAVDAINAGGAGAA"
     gene            complement(21248..21988)
                     /locus_tag="PLW13_03420"
     CDS             complement(21248..21988)
                     /locus_tag="PLW13_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69449.1"
                     /translation="MARAVTRKAKITAVSAVPEKQTDAKPARRASSPGRPARLSREMI
                     LGKSVELLAEHSIEGFTLARVADALDTVSMALYNYFPSREALLNAVADHICKQFRMPK
                     AKAGQAWQDTLRAWLWAVKKHAEKNPVIFKVMGVDGRTSAGWLSITHTVGKTLYEQGL
                     RDRDLAAAIWLFCSQAIGLLLSEYGQGSFRSSLSLSQLEQLGPEQQAFFLQLRNYHVP
                     LTTEELLEEGFNDLIANLERKIALLGKK"
     gene            22149..23714
                     /locus_tag="PLW13_03425"
     CDS             22149..23714
                     /locus_tag="PLW13_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69450.1"
                     /translation="MNDYHFEPLGLIDYSFLAQETPERHMSVAGVAIFEAGPLRRPGG
                     GIDFAAIRNATAVAIEQIPRYRQKLMWRAHRELGSYLGFGDGAHENESLPPVWVDDPH
                     FDIDYHMRHTALPRPGSDAQLKTLAGRIASQPLDRSRPLWETWVVEGLDGERFALISK
                     LHHCMIDGMSGMDAATILSSTSPDFVPGKPSPWRPRRAPTRQELERELQREQLSAPLR
                     MLRDLLQRDRAELAGEIVHRIKGIGETFAETAGATRTATPLNGRNGPHRAIDWMTVPL
                     QRIKAASKAMECTVNDVVLTILTGALREYLLQTDFAPGSAPFRATVPVSIWGDRRKGE
                     IGNQIAVWMIELPLGKSAPRAQLRAIREVTARLKHSDRPLGVKTLESVLSLAPGLVSL
                     AVRNAAGPSNTLITNIPGPQVALYQMGAKQLAAYPIVPLLEDMGIGIGVMSYNGLTCW
                     GITADRNIVPDVGAFMDALAKSLAAVDKAAGMVAKASPRKLVANKKSAPAKQKTPVKK
                     TAAPNTAPSRKPR"
     gene            complement(24114..24869)
                     /locus_tag="PLW13_03430"
     CDS             complement(24114..24869)
                     /locus_tag="PLW13_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69451.1"
                     /translation="MYAMTGGATGIGAAIRTRLRAAGHEVIVVDIKDADIVADLGTAQ
                     GRRAALDGIRARAGDGLDGFVPCAGLSGNVADHALMASLNFFGTVELVEGLKDLLVAR
                     KGAVVLIASNSAPHPTSEDYVNALLAGDEATARTLAGAMQGHPVYSGSKLALARWMRR
                     NMREYAAAGVRLNAIAPGYTRTPMTSAVEADPAYADAIREFLAATPLGRAGQPEDQAD
                     AVLFLLGPAASFICGSVLFVDGGYDAMTRPDAF"
     gene            25251..27239
                     /locus_tag="PLW13_03435"
     CDS             25251..27239
                     /locus_tag="PLW13_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69452.1"
                     /translation="MNYFLTGGTGFIGRFFIENLLQRQGTIHVLVRESSQHKLEELRS
                     RFPLAAGRIAAVTGDITRPGLGLCAADLERLKGSIDHFFHFAAIYDMAASEASQNAAN
                     ISGTLHAVNAAAQLGAGCFHHVSSIAVSGLYRGTFREDMFEEAENLDHPYFRTKHESE
                     KVVRQQCRVPYRIYRPAAVVGHSRTGEIDKIDGPYYSFKTIQRIRNVMPKWMPLVGVE
                     SGVNNIVPVDYVVNAIDYLAHLEGQDGGCFHIVDTEHHSTGQMMNIFAEAAHAPKFSV
                     RFDPKIFNFMPATLVDLLAKLPPVKRLKAAVFERIGMPESASILTKYATKYDSRHTRR
                     LLEGSGIAPPRLASYAPRIWDYWERNLDPDLFVDRTLEGQVKDRLVLITGGSAGIGKA
                     AAIRLAGAGARVVITARTQEKLDETCAEIATQGGQAFSYVCDGADLESVDHVMRQIEA
                     DHGGVDILINNAGRSIRRSVALAYDRFHDFERTMQLNYFGALRMILRVLPGMDKRRRG
                     HIVNISSIGVLGHPPRFSAYIASKSALEAFSRCAAAEYSDRNIHFTNINMPLVRTEMI
                     APTKIYEYAPVMEVQEAVDLIVEAIIHRKARVATGMGVFMSAMGALFPKVAEIVNNAT
                     FRMFPDSEAAKGLPAGSAPSEVTSEQVAMAQLMKGVHF"
     gene            27314..29017
                     /locus_tag="PLW13_03440"
     CDS             27314..29017
                     /locus_tag="PLW13_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQY69453.1"
                     /translation="MSSIKTEYDAAVARITAPGAPFELVNATIDGVEYRLYRNAPRSL
                     AEVFAQATAHGEREFLVYEGERWSYTQLLAQGARIAHALRRHCGIRPGDRVAIAMRNY
                     PEWMSAFIGIATLGAVAVPLNSWGQARELAFALDDAGARAVFCDQQRCDQLATHLAAT
                     GLPAIVARPTGAGLSGGTRSLEDFVAEIDETAFPAVDIGPEEPALMMYTSGTTGTPKG
                     ALSTQRAVCQSLMNFDCQAMASAMINPEAIGAMMKKGFPPTQLLAVPLFHVSGCHAVF
                     LCALRGARRVVMMYKWDVRKALELIQRERVTALSAAPGMLMELMESPDFERFDTSSLS
                     GIGGGGAATPPRAARLFMDKVADSYPGAGWGLTETNSNGSACTGRPFREKPGTAGFLH
                     PTVDLSIRDEEGNELPQGVPGRLWVRTPTLVSGYWNRPEANETEFRDGWFDSGDIGYL
                     DDDGYLFLSDRAKDLVIRGGENIYPAEIEGAILECPEVDEVAAFGVPDETWGEQLAVV
                     VHATPGTTISVEDVKRFAAAKLAHFKVPHYVEVRSEHLPRNATGKILKKDIRAEFLRK
                     L"
BASE COUNT         4737 a        10037 c         9637 g         4756 t
ORIGIN      
        1 ccgatgtcgt acacctgcga caccaggcca atgaagctgc ggtcgttcat cggatcggtc
       61 ggcatctcga acgccacgcc ccgcttgcgc atgtcatcga tgaggatgtt cagcgcctgc
      121 tccgccgaga tgtcgtcgct gtggtccatg tagaccttca tctcgtcgaa gtccttgtag
      181 agccgcgcgc cccagtggac gaggaagcgc atctcctcgg cgggaatctt ctggttgacg
      241 acatcgcccg tggtgatgcg ccagccgccc tcgacctccg gatccgcacc gaatctcgac
      301 gagatgtcga gcccggcggg tttgcgcatg gcgagcggcc cgcaggcctg gccgtggtgg
      361 aacatcatct cgttctcgac caccaccgca cgcccccaca acggcgcgtt gatctgcaac
      421 ggttcgcccc tgggaccgtc gggccagcaa ttgaagccgc caccgatcct gcccttgtag
      481 taccacgcga tgacctgccc tttcttcacc tgccagtgac ggaacaggca tgacttcgcc
      541 atcgtgagca acaaccacag cggcgtgttc tccatcgtca tgccgcggaa gacggtgccg
      601 tccaggtgcg gcgggccgcc gatgggcgaa ggaccctgga tgttgaagag aaaggtctcg
      661 ggctcggcgt acttcgcacc ccagtactct cgcaccagcg ccaggaacct ggagttgtag
      721 tagcagtcct cgagctccgg gtagaagcac gcgcccccgc gcgccaggta gccgcgaaag
      781 accgggttga ggatcatgtc ccaggtgagc tcgacgcctt ccgggagcat ccccgacgtc
      841 gtggcgatca cctcctcggg cgtcttgaag ttctccgcca ggatgagctg ccaggggccg
      901 ttgttgcgaa cgacgtcgag cagacggcga tgctggtcct cggtataggc atcctcgacg
      961 atacggggcg gcgcgacggg ccggaacttg tcggcgtttt cggtaatcac ggcgtatctc
     1021 ctcgaatggg gttgtcggcg tggcgccacg tcagatggtc agcattgcca tttcaggctc
     1081 gaccgcaggg cccatttccg ggcgccgggt aaccgtgaaa tccccggtgc gcggcgaacg
     1141 cgtgtcgtgc cagatgcgcg agttgtggcg tggaatggcc aggatgacct tcctgctgtg
     1201 gtcacgatag taaccgtgcg cgttgccgca ctgcacccag gccgtccttg ccaggttcgc
     1261 atcgacatcc tcgacgaagg ccctggccgc ggactcggtg acgtcgacgg acgccaggcc
     1321 gtcatcgacc atccactgca ggagctcgat cgcgtagtgc acctgctgct cgctgacaac
     1381 ggcgtgattc gccaccaggt agctgtacgg cgcgcccatg aacacgaagt tggggaagcc
     1441 cgggatcagc atgccttcgt acgagaacgg gtagggttcg aaggtttgct tcagcgtcct
     1501 gccgccgctg ccacgaatgt cgaactggcg cccgaagaac agcttgtagc ccgtcgagag
     1561 caacagcacg tcgcattcga tgcgggtccc gtcggcaaga atgaccgcct tctcgtcgca
     1621 ggcctccagc gcgccgcgca cgatgctcac gttgcgcttc ttgagcgtgt catagaagcc
     1681 gcagtccagg atcggccgct tcgcgaacac ggggaaagac ggcgtgagca gcgcgaccat
     1741 cccgggatcg tccgggaaat ggctcttcag gtaggcttcg cacatcatgc gaatgccgtc
     1801 gttgatcggt ccgaagccgc cggtcttcgc ggcatactcc tcgtccttca tcaccatgcc
     1861 gtgcatgtcc tgcatggcgc tcgacaggct ctgcaggcgc ttccattgca cgacgaaggg
     1921 gatgtgctcc atcgcccagc gttccagttc gtcgacggga tcgtgcgcgg cggggttggg
     1981 gatgatgtgt tccggctgtc gctggatgat caccaactca cccaccgtat ccgcgatcgc
     2041 gttcgccacc tggacgccgg aacaaccggt gccgaccagc acgaccttct tgccctgcag
     2101 gtcgaccgag tggtcccaag ccgcggaatg gataaccgga cccgcgaacg tgtccagatg
     2161 cgcgacgtcg ggataattgg gcgcgttatt gggacccacg gccatgatca gcgcctggcc
     2221 ttcgaagacg accttgttgc cgtcctggag ggcgttcacc ttccagagct ttcgctcctc
     2281 gtcccactgc gcgccctcga ccgtggtgtt gaaggtgatg cgctcgcgca gcttgtaccg
     2341 gtcagtcacc cgctccaggt acgcgaggaa ttccggaccg accggatagt acttgctcca
     2401 ggacgcgttg ggctcgaagg agaaggagta gacgcggctc ggcgtatcga ccgccgcgcc
     2461 cggataggtg ttctcgagcc agttgccgcc gaccgcttcg agtttctcga tgacctggaa
     2521 gtcgaacccg gcctgctgca gcttcacggc ggcgttgatg cccatcatgc cggcacccac
     2581 gacgagcacc ttgaagtccg cgggcggaag cctcttcggc tggcgcacgc gggtatccgt
     2641 cgtgaaaccg ccgtgttgat aggcgacgtc gatggacatc tcgggcattt ccagcccgag
     2701 cgcgagacgc gccatctcgc gaaacagcgg cttgtcgagc gttgccagga cggctgggcg
     2761 gccggatctc accgcggcac gcagcaggtc gcggacttcg cgggcgactt ccagcgacgc
     2821 ggcgctgggc tcgcgcggcg tgtcgaaagc cacgaaagcc tccttcacct ggtgctgcgt
     2881 gccttcgagc gcgggcccgt acttgcgtag cagcgatacg tcaccggtgc tctgcaccag
     2941 cgcgagcagc gcgatgaccg gatccagcgc atcgatggcc gcatcctgca tggagcccgt
     3001 gttcgtcggt tcagcgatca tttgtctttt tcctggagta ccgggtggag cgcggcgaga
     3061 cgccaatgca tttattacaa cataagatgt acttaaagac cacctcgatc ggttcgccgt
     3121 tttcaaccag gggcaggcgg gcggaacatc gcaccggact ccagtgattt cgtgacgctc
     3181 gacctgcagg gatccgatcc gtagactggt gcgcggtccc ggctgcaacg agccggtggc
     3241 gtcgtgcggg attggttcaa ctgcaatatc cagaggcgcc aacgtgtccg aaggcaacat
     3301 catcgaggtg agcgatcttc attttgcgta cggtgatcgc gcgatcctga agggtatgga
     3361 tctgcacatt ccgcgcggca agctggtcgc gatactcggc accagtggct gtggcaagag
     3421 cacgctgctg cgactgatcg gcgggcagct gcgccctgca cgcggcaagg tgctgatcga
     3481 tggcgaggat gtgcacgcgc tcgacaacga cgggctttat cgcctgcggc gcaggatggg
     3541 catgatgttt caggtcagtg gcctgttcag cgatctgtcg gtgttcgaga acgtggcgtt
     3601 cccgatgcgc gaacatacgg acatgccgga aagcatgatt cgtgatctgg tgctgatgaa
     3661 gctgcacgca gttggcttgc gtggcgcgca tgcaatgatg ccgggcgaac tctccggtgg
     3721 tatgcagcgg cgcgtcgcgc tggcgcgcgc gatcgccatc gaccccctgc tgatcatgta
     3781 cgacgaaccc tttgccgggc tcgacccgat ctcgctcaac gtgattgccg gtatcctgcg
     3841 acatctcaac gatgcactgg gcacgtcgtc ggtggtggtc acctacaatg tttccgaatc
     3901 attgaaggtc gtcgatcacg cctttttcat tttcgacggc aagatcgtcg cagagggcag
     3961 caccgccgat attctggcgt ccgacaatcc cttcgtgcgc cagttcgtcg atgcgcggcc
     4021 cgatggtccg gtgagtttcc actatccggc caaaccgttt gccagcgagt tgcgcctggg
     4081 cggctcctga atccccctgt ttgcgcagtt gccgcccagc gttcaagttc gcgaacggta
     4141 gctcgcagcc gaaaacccgg cgcgccggct tccagagccg atgtcccgca tctgtgctca
     4201 caggcccccg gtcgcgcggg ccaggcgccc gtgagtgagc tcaaccttcc ccgcgcgcct
     4261 gcgaccagcg cggcttgcgc ttctcgaaga aggcgcgggc cgcctcctcg gtctcgcccg
     4321 agagtcctgc catgatctgg ttgcggttct cgagctggat cgccgcctcg aggttcggca
     4381 cctcgagatt ggcccacatc acctgcttgg tcgcgaacac gccgaagggc gagaactcgc
     4441 agagctcgtc cgcgagcgcg agcgccgcgc tttcgatctc gccgtccggc accacgcgcg
     4501 tcacgatgcc gtagcgctcg gcttcggccg catcgatcac gcgcgcgctc aggatcaggt
     4561 cgtgcgcgcg cgaagcgccg atcaggcggg gcagcatgta gctcacgccg atgtcgcagc
     4621 ccgagacgcc gaggcggatg aactgcacgc acatgcgcgc cgactgcgcc gcgatgcgga
     4681 tatcgcagcc gagcgcgagc gcgagcccgc cgccgtaggc cacgccgttg atcgccgcga
     4741 tcagcggctg ctggatgcgc cgcaggtgcg ggatcaggct cgccatgtgg ttctgcgcga
     4801 tcagtcccgc ctgcggtccc ggcggcaggc cgtcgacgat cgtgcccggg ttgccctggt
     4861 cgcgcaggtc aaggcccgcg cagaagccct tgccggcgcc ggtcagcacc acgatgcggc
     4921 agcgattgtt ggcgtggatc gcatcgagcg ccgcgtgcaa ctcgcccacc agcgcccaag
     4981 acaacgcatt caggcactcg gggcgattga gccgcagcac cgcgacctcg ggccgcgggt
     5041 attcgatgcg cacgttgtcc attttctgtc ctcctgaaac cggatttggc cggactgctg
     5101 cgtgcgcgct cagcgcggca ccatgccacc gtcggcgaag accacgtggc cggtgatgaa
     5161 gctgccggcg tccgcggtca gcatcagcgc cgggcccacc atctcgtccg cgctggcctt
     5221 gcgcttcatc aactggatcg aggtcatcac gtcctgcgcc tcgggcgagt tcttgcgcac
     5281 catgtcggtg tccacggtcc cgggagacag cgcgttcacg cggatgccga agggttcgaa
     5341 ctcggccgcc atcgcgcgcg tggcggccat catgcccgcc ttgccgatgg cgtagagcga
     5401 tatggtcgag gagaacagga aggccgcacc ggtcagcacg ttcagcaccg cggcgtgcgg
     5461 gctctgcttc agccagggca gcgcctcctg caccaggaac accggcccgc gcaggttgac
     5521 gtccatcgac ttgttccacg cctcgagcgt gaattcgccc accggcagcg cgagcgcggt
     5581 cgcggcgttg ttgaccacga catcgatggc gccgaaggca tccaccgtgc gctgcaccag
     5641 cacgcgcagc gcgtcgagat cgccgaggtg cgtcggcacc ccgagcgcct cggcgcccat
     5701 cgcgcgcagg tgccgttcgg tctcggcgca ggcctcgggc ttgcggctgg caaccacgac
     5761 cttcgcgccg gccgccgcgt agccctccgc gagcgcgcgg ccgatgccgc gcgtgccgcc
     5821 ggtgacgatc accacgcggc ccgtcatgtc gaagagcttc ccgagtccgg cgcgatccat
     5881 gtcgagtacc ccgcgtagcc agcgtgatga agggccggcg ccggaggcgg gcgatcgctc
     5941 gcgccccggg ccggttggca caattgttta tcttacttgt aagcttcctg caacggcgaa
     6001 tgtccccgat atcgggccga aattaactta tgccacagtg aaatgagcag caattgatcc
     6061 agcgcaagtc gccgccgcca gattcgccta attttgcccg tgcgtcagca aacacggcgc
     6121 cggcatcggc cggtgatccc cgcctgagcc caggccggcg ggccatgcgc catccgaacc
     6181 tgcagacgcc tcggggaggc aagaacatga agaagccaac cgcgtcgatc gtcgccgcta
     6241 tcacgctcgc gctggcctgc gccgcaacgc aggcagcggt agacgcgcag gcggccgcac
     6301 ggctcggcac cacgctgacg ccatggggtg cggaaatcgc cggcacaccg gacggcagct
     6361 atcccgccta cgaaggcggc atcaaggagc cgccgccggg atacgatccg aagagcggca
     6421 tgtggccgga tcccttcccg gaggacaagc cgctgttctc gatcgacgcg aagaacatgg
     6481 cgcagtacca ggaccggctc atggaggcgc agatggagtt gatgcgccgc tacccggact
     6541 accgcctcga cgtttacccc acgcgccgca gcgcgatggt gccggagaat gccgccagga
     6601 acgcactggc gaacgcgcgc aatcccgagt gcaggaccag cagcgacggc gtgggactct
     6661 acggctgctg gggcggcaca ccgttcccga tcccggagaa cggctacgag gcgatgtgga
     6721 accacctgct gacatacacg tcgccgggcg agaccaacag cgtcggctat ctggtcaacg
     6781 ccaaccgcgc aaccacgatg ctgctgctgt cgcggtcgat agccgattac ccgtatttca
     6841 atccgcagca gacgccgtac gagggcgccg ggatctacta cctgcgctcc ctgtccacga
     6901 ccctggagcc cgcgcgcgag gctggcacgc agacgctgct gtggtatccg ctgcactacg
     6961 attcggacga gcagcgtgcc tggagctacc aggcgggaca gcgtcgggtg cgcctggcgc
     7021 cggagttcgc ctacgacacg ccggtcgcgc agttcggcgg cgcgatgttc ttcgacgaga
     7081 tcaacctctt cgcggggcgc atggaccggt tcaggttcga gctgaaaggc cgcaagctca
     7141 tgtacatgcc gtacaacaac taccgcatgc acctgctgcg cggcgacccc gcgcagctgc
     7201 tgggtccgca tcacttccat ccggacaagg tccgctacga actgcggcgc gtctgggtgg
     7261 tggaggccac cccccttccc aacgtgcgcc acgtggccaa gcgcaagctg ttctacctcg
     7321 acgaggattc gtggatcgcg ctcgcctacg agggctggga ccagtcggac aggctgttcc
     7381 gcctgctgct cagcaacacc atggcgaact actacaccgg cggcgttatc cagggcgctt
     7441 cggtacaggc ctacgacctt gcccgcggcc agtacgcggg gctgcagttg ctgatcgcgc
     7501 cgcgctgcta tatccgctac ggcctgccgc agcaaccgga cgcggagatg gcgccggccc
     7561 ggatgggtgc gcgcggcata cgctgagcgg gagggcgctc agcgcatcag tgcaccgccg
     7621 ttcacgttgt agacctgccc gttgatccag cggccgtcct cggacaggag catcgccacg
     7681 acgcccgcga tatcctcggg gcggccgagt cgcgtgcccg gtatgcgcgc ccgcatgcca
     7741 tccagcagct cctgcgagag ctgcccgctt cgctgcattt ccggcgtaag ggtgaagccg
     7801 ggcgccacgc agttcgccgt gatgccctcc cgcccccagc gcgaggcaac atggcgcatg
     7861 aaggcgttca gcccgctctt cgaggtgccg taggacggcc gcaccggttc acccgagtcc
     7921 gccgagcccg aactcgtgta gacgatcgca ccaccgccgc gtgcgagcaa ctgcggcaat
     7981 accgctcgcg tgcacaacag gtggccgcgc aggttgatcg cgagcgtgcg gtcgaacacc
     8041 gcgagatcca ccgagagggc gtcgctgtcg cgcatgatcg tctgcagatc ggcggcattg
     8101 acgtgcgcac cgtcgagccc gccgagccgc gcgaccgccg ccgccaccgc ggcgttgacc
     8161 gaggcctcgt cagcaagatc gatgtgcacg gcgaaggctt cgcctcccgc tgccacgacc
     8221 tcctccgcca ccgctttcgc accggcttcg ttgatgtcgg ccgcgcacac gcgcgcgccc
     8281 tccgcggcaa gccggcgcac ggtagcggca ccgatgccgc tgcccgaacc gatcacgatg
     8341 attgccttgc ccttcagcct gtccgtcatg agtactgtcc tcccgtcaga ttccggtgtc
     8401 gctcgataac gcttgccgat cctgccgatg caaccggcac gctcaacttc cgcgcgcgct
     8461 catgcgcgca ggccccagcg cggcacgggc ccgcgaggca gctgcggata aacctcgcgc
     8521 tcgaacagcc gcagcatctt ccacgcgcgc gccggctcga tgccgccgaa cagcggcgtc
     8581 aggtagagcg agctgtgatc acccagccct tgcgccagct cgatcgtctg cgccggtgtc
     8641 agcacctggt aggcagcgct ctggcgcacg gtcccggccg tcatgcctcc tgcgtaggga
     8701 ccgatcggct tgccataggc cgcgctggtc cagcgcgaat aggaatcgag cacgtgcagc
     8761 acgtgcggcg cgatccactc ccagtctcgc tccggctctt ccgacaccca caggaaggtc
     8821 ggcccctgct gcgggtacag cccgggatcg gcgttgccga gcttcaggca ctcgtcgcgg
     8881 tagggttgcc agacgtcggc ggggaacagc ggcacgagaa agccgtcggc gatgcgcgcc
     8941 gcggcgcgcg cgaccttggg cgccatgccg ccgagcagca ccggaaccgg cttgtccggc
     9001 agcggcgaaa tgcggccggg acgcccctcc cactcgaagg cttcgcccga gaaggcctgc
     9061 ccgaggaatt gcgcgatgcg gcaggtgaag tcgaagcggt cctcgagctt gcgaccgtac
     9121 atcgcgaact ccaccggccg gtagccgaag ccgagcccga gctcgatgcg accgtcgctc
     9181 acgagctgcg cgaccgcgag atcctccgcg agacgcaccg ggtcgtagca ggaagccagc
     9241 agcacggccg tgcgcaggcg gatgcgccgc gtgcgtcccg ccatcgcgct ggcgagcacc
     9301 aacggcgagg ggttgtaacc gtcctcggcg aggtggtgct cgccgaagcc gatcacgtcg
     9361 aagccgatcg cgtcggccca cgccgcgcag tccagcgccg cggcgtacag ctctcggcgc
     9421 ggggcgccga aggccggcgc acgcatgtcg aagatcatca ccagttccac gattcacctc
     9481 cgcacggcat cgattgcaga gcccgcgaca cggacaccgg cgcgtggacg ctcaaatccc
     9541 catcagctgc gccgcggagc gcccgaagaa ggcctcgcgc gcgcccgggg cgaggccggc
     9601 gagctgcgcg tcgtagagcg cgaccgcttc ctggtggccc tcgacgtgcg ggaagtccga
     9661 ggagaacacg accaggcctt cgggaatgcg gtcgagcgtc gggcgcagca cgtcctgctg
     9721 gtgcaccacc gagacgcgca cctgccgctg catgtactcg ctgggcttca gcggcagctc
     9781 gtagggcgcc atcgtcagca ggcgcgggcg cgtgttgtcg gcttcgaggt cgatggtgtc
     9841 gaggaagtgc ggcagccagt cgatgccgag ctccgagacc aggatgccga gcttagggtg
     9901 gcgctcgagc acgccgccga agatcatcgc gccgagcgcc atctgcggca ccagctggcg
     9961 cgccagcgca gcgaggcggt agaacgccgc gaggtcgccg ccgttgttgg cccagcccgg
    10021 gtgcatcgcc gcacgcccgc cgccgacgtg gaacatgatc gtgacgccga ggtcttccgc
    10081 cgccgaccag aagcgttcga agtcggggtg gttcagcgcc ttgccgccgt gcgggtcggc
    10141 cttcacgaaa gccacgcgcg agccgccggc gcgcatgcgc gtgatctccg cgacgctcca
    10201 gtcgacgtcc atcagatcga ccacggtcgc gggaatgagg cgctccgtgt agccgtgcag
    10261 ccgctcggcg gcccaacggt tgtacgtttc gagcgccggc agcgcgagcg agggatcctt
    10321 gcgcatcgca tggcggtacg ggtggtaacc agaactcggc agcaacacct gcttctcgat
    10381 gccgcgcgcg tccatccagg caatgcgcgc ctcgggctcg tgggccgcgg gcaggaacga
    10441 gcccgactcc accgcgctcg gcggcggttc gccctcgccg ctgaggaagc gctcgtagga
    10501 ctcgcgcaca tagtcgggca ccagcgccag cggatcgggg cgcagctgcg gcggcaccga
    10561 ggcgaacaga tcgccgacca cgatgtcgat gaactgctcg gccgccgtca tcctgggcag
    10621 cttcgccgcg agcgccgggt tggcctgctc gaaccagccg tagggttcgt tgaagtggct
    10681 gtcgacgtcg atgatgcgca tggttctccc gccgcggctt gcgatggact taattaaacg
    10741 tttccgtatt attatcgcgg accggaaaca gcgtcaacca ggtgagcagg agatgtcgga
    10801 caggatttcg cagggctcct cgggggcgcg cagctcatcg cgccgcggtc gcccgaccgc
    10861 cgcggaggcg aagcacaaga acgagcgcat cctcgcggcg gcgaccgcgg ccttcgtcgc
    10921 tcacggcatg caggcaagca tcgaggcgat cgcgcaggtt gcgggagtgt ccaagcccac
    10981 gctgtacgcg cgttacccgg accgcctcgc gctgttcgag gccgtgctcg gtcagctgca
    11041 ccgcaaccgc ccgagcatgg atacggtgat cactgcggag atgccgctgg ccacggcgct
    11101 cgagcgctac gcccgcgcgc tgcttcccgc gctgaccagc ccggaagtgg gcgccttcca
    11161 ccgcttcacg ctgcagggat cgccgctgga gcggcaggcg cggcgcgtga tgatccgcga
    11221 cttcaatgcc gccttcgtct caccgctggt cgattacctg aacgcatgcg cctccagcgg
    11281 caacatgggc gcgggcatcg atgcgacgac cgccgcgcgg atgttcgtgc accaggtcat
    11341 cgggctcatc gactaccacg tcgcaacgcg ggacagccgg gctgcggcca tccgcggcgc
    11401 gcggcgcgaa ctcgcctccg tcatcgccat cttcatcggc gggctgcagc cgcgcgccga
    11461 cctcagccct gcagcatctg cacgacctcg atgagccgcc cgtccacgtc ggccacgaag
    11521 ccgacgcgga caccgtgctc cggatcgtcc ttgggtgcct ggatgatcct gccgcctgcg
    11581 gccggtacgc gtgccaccaa ttcatcgacg ctggatgtca cgaaaccgag gatcacgtcg
    11641 cttgcggcag gcgccggacg ccggggccac ttccagatca ccagcggcag atcgccctcc
    11701 atgctcgtgc ccttgaccat gatgatttcc gacatcggct cgccggcgac gtccgactgt
    11761 acgcgctgga attccttgta atcgaatacc cgcttgtaga aatcggcggt ccgatccaga
    11821 tcatcgacga cgatcttggt gaaactgtag ttgttgctca gggtgctcat gaaccttctc
    11881 cgtttggtta ttgcagggga gacggcgtgc tgcgcaggca gcccgccgtg atggccatca
    11941 gagatccgcg actttcagca cgatcttgcc gcgcgtgtgg ccgccctgga tgtcacgatg
    12001 cgcagctgca gcctcgcgca gcgcgaactc gcgcagcggc ggcagcttca cgtggccggc
    12061 gaccagcaat tcaccgatct tcgtgagcgt gggcccgcaa ccgatgtcgg tcatcgtcga
    12121 gaacacgcgc ttcacgcccc tggccgcacc ggcggccgcg gccgccggga tgtcgccgtc
    12181 gtcgttcagc gtcgggatgc tgacgaaggt gccgcccgca cgcaccaggt ccagtccgtt
    12241 cggcagcgtg ctcacgccca ccgcgtccat caggtaatcg agcccgcccg gcgcccacgt
    12301 ggcaacggcc tcggcgatgt tctcccggcg gtagtcgatc acgcgctcca cgccgagctc
    12361 gcgcaggtat tcgacgttcg gcgtgctgca cgtggccgcc acgtgcgcgc cggcccagcg
    12421 cgcgaactgc accgcgaagc cgcccagtcc gcccgatccg ccgtggatca gcgcgcgctg
    12481 tccggccttg agctcgccgc gatcgaacag gccctgccac gcggtgagcg cggcaacggg
    12541 caacgcggcg gcctgcgcga agctcagccc ctgcggcatg tgcgccacgc ggtccgcggt
    12601 cgcgatcgcg aattgcgcgt agctgccctg cccgccctgg ccgtgattgg tgggcgtgaa
    12661 cacgcggtcg ccgacattga aaccgctgac gccggcgccg agcttgtcga ccacccccgc
    12721 ggcatcgaaa ccgatgatgt acgggaacac gtaggggcga aacgccgcga gcatgccctg
    12781 gcggttcttc cagtcggcgg ggttcacgcc ggcgtaggcc acgcggatac gcacttcgcc
    12841 ggggccaggc tcgggcgtgg cgatctcgcc gtattgcagg acctcggggc caccggtctc
    12901 gttgatgatc atcgcaaaca tggggttttc tcctgtgtcg ctcagctgcc ttcgcccgac
    12961 gccacgatcg ccggatgggc gtaaggcggg atcaccggat cctcgatgcg ctcgcgcccg
    13021 tccagcgtgt ggaccggcag tttgtgcgcc agcgggaagt gcaggctgta ggcagtgagc
    13081 ccggtgcgct cgcgcgccgg caggtggcac agcgcgagcc cggtctcgac gatgtactcg
    13141 atgcgctccg acgggtattc ctcgggaatc aggtcgacgg cgcccggcgt gcggatcgcg
    13201 gtgctcggcg ccagcgtgtt cacggcgatg ttgtcgggca gcagctcggc ggccaacccc
    13261 tgcgtcatgc gatgcacggc ggccttgatg gccgcgtaga ccgtggcgcc accataggtg
    13321 gcccattcgt cgtagggccg tccgggcggc tgcgccgtga ccgaaccgag caccacgatc
    13381 cagcccgcgc cgcgcttgcg catctcgggc acggccttct gcaccagcgc gaagggcgcg
    13441 cgcaggtagt gctggatggt gcgatcgaac atatccagcg gcatctcggc cgtgggcgcg
    13501 tattccgcct tgccgccgct gtggatcagg atgtcgaggc caccggcgag cgccaccgcg
    13561 cgtccgacca ggccctcgac atcggccgga ttctcgatgt ccgcgaccag cggcatcgcg
    13621 cgcccgccgg ccgcctcgat caacgccacc gtctcgcgca gcgtgccggc gtactggccg
    13681 gccgtatcca ggctgcgcgc actcaccacc acggtggcac cctcggcccc caggcgctgc
    13741 gccaatgcgc ggccgatgcc gcggcttgcg ccggtgacca gcgcgatctt tccttcgagt
    13801 aacttggcca tttttctacc tcttcccggt tttgaatgtt gatcggcatt catgccgaca
    13861 ggtgcctttc ccaatgctga tgtcgccatg aatggcgacc cacggggcga cctacagcac
    13921 aatgacgctt tgcgccagcg gtgcgaacgc gtggcacaac gcggtgtcga tgatctgtgc
    13981 gcccgattcc ggatcgaacc agcggtcgat gtgcgcccag tggtgcggat gcaccatgta
    14041 gtccacgttc agtccatcga gcgtcgcgta ctcctgctcc cacacgaaac tccagggggc
    14101 gcagtccggc gcgtcccaag cgagcgccac ggcgcggctc aggcgccagt tgaggatcgc
    14161 cgggatctgg gcggccatct cgagggtgtc gcgcgcgaaa cgcgcctgca gctccgggga
    14221 aacatccggc agcaggcgga acagtgccgt gcgcttcacg ccgctggcga gcgcgggtgc
    14281 gcgtgcaccg gcgcccaggg tctcgaggcc gagcgcggcg cgcgcgcggc agtggcgatc
    14341 gagcgcaggc agcagttgct cgcgccagaa cgacgaggcg cgcgccgtct cggccgtggc
    14401 gcggtcgggg tacacgaggt cccaggtgta atggccggcg ccgtaatcgg gcacgaagtt
    14461 cgcggcaagg ctcaccgtac ccaggccggg cagttccgtc gcggcgcgct ccagcagcgc
    14521 gacaagggcg gcgcgctccg cggccgtgat ctcgttcttc aggtagagct gcgcggtctc
    14581 gcgccaggcg agcggctggc accggtacgc cgtggtgtcc tcgggccccg gcaacgccgc
    14641 atcggccggc accaggtaat gccgctcgcg ctctagtacg aacgtgtcga gctcggccca
    14701 gaaagccacg acggcgggat cccccgaggc atagcgcgcg gcccagaaag cgcccgcgtc
    14761 ggccagcgac cagcgcagcc agagggtgtt gggccgctcc tgcagctgca gcggcgggga
    14821 gaccagttcg tcgatcagcg tgaggcctcg cgccgcggcg ccgggcaggt agcgttcccg
    14881 cagcatcgtc cgcaggcgcg gcaggtccgc gggggcgacg tcgatacgat cctggacaac
    14941 gatcatgtgc agcctctcgt ctgattttcc atgcgggtcg gcattcgtgc cgatttcgac
    15001 ccggcgggtt gggtggtgcc gcgtctctgt cgggctgaag cccgacctac gggggcaggc
    15061 cgacctgcgg gttcttgtcg ggctgaagcc cgacctacgg ggccggcccg acctgcgggt
    15121 tgtggcgcgc tagtgcgcgc cgagcagggc cagcgtttcc gctgccatct gcggtttggt
    15181 cagcccggag aggtgcgcgg cgcgctggcg cgcgaactcc gcggtcatct cgggatgcgt
    15241 cgagacccag aactcgccgg cggcaatctg gcgcaggatg cgctgcgccg cctcgagacc
    15301 gctgatgccc gactgctcga gcatcgcgcg catcacctcg cggtggtgcg ccaccatcgg
    15361 gtcggtgccg ggcttcgagt cctcgaaaat gcgtgtggcc acgggtccgg gcaggatcgc
    15421 cgagacctgg atgggcgcct tcttaacctg catctcgagg ctgaggcatt cgctgaacga
    15481 gagcaccgcg tgcttgctca tgacgtagga ggtctgcacc ggcatcatgc cgagaccgcc
    15541 gatcgaggag gtgttggtga tgaacgccgg cttgccggcc gcgatcatgc gcggcgcgaa
    15601 ggcgcgcacg ccgtgcacca tcgcgttgat gttgatcgcg agcgtcttgt cccaggtcgc
    15661 ggccgacatt tcccagacga agccgagggt ctcgacgccg gcgttgttca tcagcagtcg
    15721 cacgtcgccc caggtttcgt gcgtgatcgc ggcgagccga tctatcgcgg cagcgtcggt
    15781 gacatcggtg ggaacggcca tcgcctcgcc acccgcggcg cggatctcgg ccgcgaccgc
    15841 ttccatgcgc tccaccgcga tgtccgcgag caccaccttc atgcccagcg ccgccgcgtg
    15901 ccgcgccagg ccctcgccga tccccgagcc cgcgccggtg atcaccgcca cgccgccgcc
    15961 gaataccgcg cgttccttgt ccatgttcat ggctctcctc gactgaagat tattgttcga
    16021 gcggcacagc ctaggcgacc gcaggccggg cgacatcacc cgttccgact agacggggac
    16081 gccgccgggc cattccgccc gactgtttaa cttatatcta agagaagtgc aatcgccgag
    16141 aagcgcaatc gccgcggctt gtggcaaccg gccggcgcgc tgcgcggggg accggccggg
    16201 agcgcgaacc cccgcgaccg gcccaccatc gggctcaggc gagcgcgggg ccgaggaact
    16261 cgcccggcat cgccccggtg aactgccggc cgtcgaaggt ggacacacca ttgaccagcg
    16321 tgaggcgcat cggcgccggc gggcgcgtga agcgccacgt ggtgccgccc ttgccgtccg
    16381 gcacgtcgaa gcgcttctcc atcttgcgat nnnnnnnnnn tcgaggtcga acaccgcgat
    16441 gtccgcgcgc ttgccgaccc tgatctcgcc gcggtcgtgc aggttgaaga aattcgcgag
    16501 cttgccggtc atcacgtgga tcgcctgctc cagcgtgatg cggttctcct tcacgtactt
    16561 gctgaacagc aggatgttct cgccgccgcc gcagagcatc tgcaggtgcg cgccggcatc
    16621 cgagatgttg cccaccgtct tcggttcgag cagcaaccgc gtggtgagcg cctcgtcctt
    16681 cgggaaaggc gccatgtgca cggtggaatg cgtgccgttc tccaggatcc actgcgccat
    16741 cgcgtcggag cggtgcaggc cgcggctgtc ggcgaactgc ttcagcgtga tgtcgaccgg
    16801 gccggtgccg ttcgcggaat cgagcaggta gagatcctgg gggttgttca tcggcgagtg
    16861 tttccacgcc ttcgtgtccc aggactcgcg cgcccgcgcc cgccagccgg gatcggccag
    16921 caaccgcgcc ttttcctcgt gcgtctcggc cagcaccacc tcgtgccaga cgtagtcgtt
    16981 cgactgcgcg aagatcaacg acttcaccag gctgagcgtg ctggtcggcg agacgtgcgc
    17041 gtagcccggc cacaggtcca cgccggcgtc ctgcatcttc ttcaccgagg cttccatcgc
    17101 cggaaggatg tctttctgga actccagcgt gggaatcgcg ccggcgatct gcacgcgcgt
    17161 cctgcgtccc ttcagcagtc gctccaggcg ctccagcgac tgcggcccgg tcttgcgcat
    17221 cgcggtatcg acgatgacct ggtagctcgc cgccgggtag cgctcgagga cgtcgaacaa
    17281 cgcggtgaac tccgcgtcgt cggccaccag ggtcgggatg ggccggttgt cgccgtcgtg
    17341 gtcgtgcata ttgtccgaga gccccagcgc gccggcggcg agcgcgtcct cgagcagttg
    17401 cgccatgcgc gcggtctccg ccggcgtggc gacgcgctcc caggcgtcca ggcccatcac
    17461 cgcgatgcgg atcgcgatgt ggccgacgaa tatcgcgtag ttcgccggca ccttgacctt
    17521 gctggtcagc gaggccttgt attcggacca tgtctcccaa tcccacggca cgttctcgag
    17581 gaacggcccc atcgggatgt cctcgaagaa cgagaagatc ttcaccatct ccagctgcgc
    17641 ggccttgtcg cgcgacatcg gcgcggcggc aaacccgcag ttgccgagga tgatcgtggt
    17701 cgcgccgttg ccgggcagcg ggtcgaggtc gggctgccac cacatcgtgg catcgtaatg
    17761 ggtgtgagcc tcgatgaagc cgggcgtcac gtagcagccg ctggcgtcgt gcacctcttc
    17821 tgcggcgcgc ggggcgagcc cgcggccgat ctccgagatc aggcccgcgg tcacgcgcac
    17881 gtcggccggg taggccgcgg cgccggtgcc gtcgatgacc gtgccgccgc gaatcaggat
    17941 atctgccatg gtcttccccc cgtttcatgg tgatcgaatg tgcgaggaca atattccgaa
    18001 taccattcgg aatataggca gaaaacatta cagtttgtta accaactgcg ccggcgtcga
    18061 cgctccgggg ccccggggcg gagcaagctc agcgctggaa catcagccat ttggattaga
    18121 tggcctgccc gcggcacctc tatactcccg cttcctcata accacaccac gggagtacag
    18181 gcatggcggg caccggcagg ctgcagggca ggatcgcgat catcacgggc gcggcccagg
    18241 gcatgggcgc ggaacacgcg cgcgcattca tcgccgaagg cgcacgcgtg gtgctgaccg
    18301 acctgcagga gcccgcgggg caggcgctcg ccgccgagct cggaccgaac gcgctgttcg
    18361 tgcgccacga cgtgagcaag gcgcaagact gggatgccgt gatcgcggcc acgcgcacgg
    18421 cattcggccc ggccaacgtg ttgatcaaca acgccgcgat gtatttcatc tgccccgtgg
    18481 acgaggccga tcccgagcgc gtgcgcaaac tgctcgacgt gaacatctac ggcagctggc
    18541 tcggcatcag caagctcgcc ccggtgatgc gcgaggccgg cggcggctcg atcatcaacc
    18601 tgtcgtcgct ggcggggatg cgcgggatcc cgtggcacgg catctacggc acctcgaaat
    18661 gggccatccg cgggctgacc aagacggcag cctacgacct cggcccttcg ggcatccgcg
    18721 tcaacgcgat attgcccggt gcgatcgagg aaacgggcat ggcgggcggg ccgctcgacg
    18781 agacgcagct ggcggcaatc ccgctgcgcc gcacgggacg caagagcgag gtatcgcagc
    18841 tggcggtgtt cctggcctcg gacgagagcg cctatatcac cggcggcgat cacgtgatcg
    18901 acggcggccg cgcgctctgg tgagccgcgc ggcacgccgc gccggcgcgc gcctcaggcg
    18961 gcgccggctc ctcccgcatt gatcgcgtcc acggcgagca tcgcgccgcg catcgccccc
    19021 tcgatatagc ccacgccggt accgtcaccg acctcatgca cgcgcaggcc cgcggcacgg
    19081 aaggcctcgg cctgcgagga atcgccggtc gcgcccttgg ccacgatcac gtggtcggcc
    19141 tccaccgtgc gcgccgcgcc gccggcatcg gtgaagctca cgcggccggt ctcgatgcgg
    19201 atgtccttcg cgctcgcgaa caggccgagc ccgtgctcct tgagctcggc gagcaggcgc
    19261 atgcgccgca ccagcgtcag ccccttgccc aggcgcggca cctcgtcgat cacgctgacc
    19321 ttgcgcccgc gctcgatcag gaactccgcc agctcgagcc cgaccagctc gccgccgatg
    19381 atcgcgacgt tcctgccgag cggcatccac gcatgcgtgg ccttgcgcac gaaatcgagg
    19441 ttcgccgtga gcccggtcgc cgcgcccgcc ttggtggcga tgcgcgtcag cagcgaggtc
    19501 ttctgcgcca gcgcctcgga gctctcgcca agcatcatct tgcgcatgtc gtcgccgctg
    19561 aaaacatgcg ccagatccgc gcccgggatc ggcggcatgt tgcgcagcgc gcccgtggcc
    19621 acgatgacct cgtcgggttt cagcgacttc agcaactcgg gggtggcaac ggtgttcagg
    19681 cgcacctcga tcgtggcctg ctccacctgc aggcgcagcc agtcgagcag gcgctcgttc
    19741 gcggcatagg ccagcgaggc gaagcgcagc gtgccgccga gacggtcgcc cttctcgatc
    19801 aggatcacct cgtggccatc gcgcgcgaga cgccgcgccg actccatgcc gccggggccg
    19861 ccgccgacca ccaccacgcg cttgcgcgcg ccgggcttcg gcgcgtccac gcgcaggtac
    19921 tcgatgccgc actcggcgtt cacggcacag cgcgtggact ccagcgtgta gatcgcgctg
    19981 acgcaggtgt agcagtagat gcacgggcgc acctgctcgg ccttgccggc cgccagcttg
    20041 ttcggcagtc cggggtcggc gagcagcttg cggcccatgc cgaggaagtc gagccggccg
    20101 gcggcaatct cggcgtcgcc gtgctcgggc tcgacccgac ccgagcagat caccgggatg
    20161 tccacgatct tcttgatctc ggcgcccgcg ggcaggttgg tgttgggctc gtgggggatg
    20221 ttcgaggccg agtgcagctt gccgatgccg gtgtcgtggt aggcggtcac ggtgatcgcg
    20281 tcggcgccgg cttgctcgac cagcttcgcc gtcaccctgg cgtgctccag cgtgataccg
    20341 ccttccttgc ccacctcgcg cgagtccatc ttcacgatca cggcgaaatc cgggcccacg
    20401 gccgcgcgta ccgcgcgcac gacctcgacc aggaaacgcg agcggttctc cgtgctgccg
    20461 ccgtactcgt cggtgcgctt gttggtcttt ggcgacagga acgaagacag caggtagccg
    20521 tgcccgccgt ggatctcgac cgcatcgcag cccgcctgct tcgcgcggcg cgcgccggcc
    20581 gcgtaagcat ccacgacgac ctggatgtcc tcgtgggtca gcaccttgta ggtgggcatg
    20641 ccggcgctgg caaaggcctg cagctcctcg agcaggaagc cctcggtgaa gtcgcccttc
    20701 atcggctcgg gcaccgaggg gcaccactgc gggcgcccgg ccttggtgtc gtcgccggct
    20761 accagcccgc cctggtgcag ttgcacggcg tacttcgcgc cgtggcggtg cacggcctcg
    20821 accacgcgcg ccaggcccgg gatgaacctg tcgtcggaga tgccgatctg ccagggctgc
    20881 acctggccga tcgggtacat cacgccgcag gcgccggaga tgaccagccc ggcgccgccg
    20941 cgcgcctgct cctcgtggta ggccaccacg cgatcgccgc tggacccgtc gtcctcgctg
    21001 aaattgacac ccatcgccat gaccacgatg cggttgcgca attgcatggc gccgatgcga
    21061 ccgggcgaga gcagatggga ataggcgggc tgggacatgg gcgctccttg gagtggttat
    21121 ctggcggggt ggcagtatag cgtccgcccc cggctgccgt gggtccggct tcagccggac
    21181 atttacggtt tccgcgcaca cgtcctgctg aagccggacc cacgcgggtt tctcgtacgc
    21241 ggaaaggcta cttctttccg agcagagcga tcttgcgctc gagattggcg atcaggtcat
    21301 tgaagccttc ctccagcaac tcttccgtcg tgagcggaac gtggtagttc cgcaattgca
    21361 ggaagaatgc ctgctgctcg ggcccgagct gttccagctg ggacagcgag agcgagctgc
    21421 ggaaactgcc ctgaccgtac tccgacaaca gcagaccgat cgcctgcgag cagaagagcc
    21481 agatggcggc ggcaaggtcc ctgtcgcgca ggccctgctc gtatagcgtc ttgcccaccg
    21541 tgtgggtgat gctgagccag ccggcggagg tgcggccgtc gacgcccatg accttgaaga
    21601 tgaccgggtt cttctccgcg tgcttcttca cggcccatag ccaggcgcgc agcgtgtcct
    21661 gccaggcctg gcccgctttc gccttcggca tgcggaactg cttgcagatg tgatcggcga
    21721 ccgcgttgag cagtgcctcg cggctgggaa agtagttgta gagcgccatc gagaccgtat
    21781 cgagcgcatc ggccacgcgc gccagcgtga acccctcgat cgaatgctcc gccagtaact
    21841 ccaccgactt cccgaggatc atctcgcgcg acagccgcgc cgggcgcccg gggctggacg
    21901 cccggcgagc cggcttcgcg tcggtctgct tctcgggtac ggcgctgaca gccgtgattt
    21961 tcgccttgcg ggtgacggcg cgtgccatgc gggaatttcc tgggaccgga gcggatgaat
    22021 gccccatgtt agcaattccc gcgaattctc ttcaaagtaa gtttgttacg cgccggcgcc
    22081 agtgcattga cttcgcgccc ggggtgcggg agggtcgtgc acacgacaac agcctgcgga
    22141 gtcgcgccgt gaacgactac cacttcgaac ccctcggact catcgactat tccttcctgg
    22201 cgcaggaaac gcccgagcgg cacatgagcg tggccggcgt ggcgattttc gaggctggcc
    22261 cgttgcgccg tccgggcggc ggcatcgact tcgcggccat caggaacgcg accgccgtcg
    22321 cgatcgagca gataccgcgc taccgccaga agctcatgtg gcgcgcgcac cgcgaactgg
    22381 gcagctacct gggtttcggc gacggcgcgc acgagaacga atcgctgccg ccggtatggg
    22441 tggacgaccc gcacttcgac atcgactacc acatgcggca cacggcgctt ccccgcccgg
    22501 gcagcgacgc gcaactcaag acgctcgccg ggcgcatcgc ctcgcagccg ctcgatcgtt
    22561 cgcgaccgtt gtgggagacg tgggtcgtcg agggcctgga cggcgagcgc ttcgcgctga
    22621 tctcgaaact gcaccactgc atgatcgacg gcatgtcggg catggacgcc gcaaccattc
    22681 tctcctcgac ctcgcccgac ttcgttcccg gcaagccctc gccctggcgc ccgcggcgcg
    22741 ccccgacgcg ccaggaactg gaacgcgaac tgcagcgcga gcaactgagc gcaccgctgc
    22801 gcatgctccg ggacctgctc cagcgagacc gcgcggagct ggccggggag atcgtgcacc
    22861 gcatcaaggg cattggtgaa accttcgccg agactgccgg cgcgacgcgc acggcaacgc
    22921 cgctcaacgg ccgcaacggc ccccatcgcg cgatcgactg gatgacggtt ccgctgcagc
    22981 ggatcaaggc ggcgagcaag gccatggaat gcaccgtgaa cgacgtggtg ctcaccatcc
    23041 tcacgggtgc cttgcgcgag tacctgctgc agacggattt cgcgcccggc tcggcgccgt
    23101 ttcgcgccac cgtgcccgtg agcatctggg gagatcgtcg caagggcgag atcggcaacc
    23161 agatcgcggt ctggatgatc gagctgccgc tggggaagtc cgcgccgcgt gcgcaactgc
    23221 gggccattcg cgaggtgacg gcaaggctca agcactccga ccgccccctc ggcgtgaaga
    23281 ccctcgaatc cgtgctgagt ctcgcgcccg gcctggtgtc gctggcggtg cgcaacgccg
    23341 ccggcccttc caacacgttg atcaccaaca ttcccggccc gcaggtcgct ctttaccaga
    23401 tgggcgcgaa gcagctcgcg gcctacccga tcgtgccctt gctggaagac atgggcatcg
    23461 gcatcggtgt catgagctac aacggcctga cgtgctgggg cattacggcc gacaggaata
    23521 tcgtgcccga cgtgggcgcg ttcatggacg cactggcgaa gtcgctcgcc gcggtggaca
    23581 aggcggcagg catggttgcg aaggcctcgc cacgcaagct cgtcgcgaac aagaagtcag
    23641 cgccggcgaa gcagaaaacg ccggtgaaga agacggcggc accgaatact gcaccctcgc
    23701 gcaaaccgcg ctgattgcca atgcccggct cgagagggtg tcgcaaagcc atcggtgctg
    23761 tggatagcgg cccgaccatc ccgcgtatcg cgagccaccg gcgcccgagc accctgtcgc
    23821 cgtcctggcg acagagtgtc cgccacaccg atgggtgctc gaacccctcg ccgtggtgga
    23881 aaccctcgga aagacgcccg ttcccggatt tgctcgatgc gacacgcggt cgaccgagcg
    23941 gctgtgctgc ccgctgttgg cttgcggggc cttgcgccgc aggacgcggc gcggagctac
    24001 agggacgtac tcgtgcgtgc cccgcagggc aatagcgggc agcacagcca gcgacgcacc
    24061 aagccgctcg ctcgcaacgt aatggctttt tgctacgccc tctcgagccg gacctaaaac
    24121 gcgtcaggcc gcgtcatcgc gtcatagccg ccgtccacga acagcaccga accgcagatg
    24181 aagctcgccg ccgggcccag caggaacagc acggcatcgg cctggtcctc gggctggccg
    24241 gcgcgcccga gcggtgtcgc cgccaggaac tcgcggatgg catcggcgta agccggatcg
    24301 gcctcgaccg cgctggtcat cggtgtgcgg gtgtagcctg gcgcgatcgc gttcagtcgc
    24361 acgccggccg cggcgtattc gcgcatgttg cgacgcatcc agcgcgccag cgccagcttc
    24421 gagccgctgt agaccgggtg accctgcatc gccccggcca gcgtgcgcgc cgtcgcttcg
    24481 tcccccgcca gcagggcgtt cacgtagtcc tcgctggtcg ggtgcggcgc ggagttcgag
    24541 gcaatcagca ccaccgcgcc cttgcgcgca accagcaggt ccttcagccc ctcgaccagt
    24601 tccacggtgc cgaagaagtt caggctcgcc atcagtgcat ggtcggccac gttgccgctc
    24661 agtccggcgc agggaacgaa gccgtccagc ccgtcgccgg cgcgcgcgcg aataccgtcc
    24721 agtgcggcgc ggcggccttg cgcggtgccg aggtccgcga cgatgtcggc gtccttgata
    24781 tcgaccacga tcacctcgtg gcccgccgcg cgcaggcgcg tcctgatcgc cgcgccgatg
    24841 ccggtggcgc ctcccgtcat tgcatacatg cccacttcgc ttctctcctg tctccgaggc
    24901 aggtccggcc tcgaccggac ggcttgccca ctgaataaac gtaccgacgc ggctcggcgt
    24961 tcatgccgac tcgtgcttgc gcatggcgga ttcggcgttg cctggccctt ctcggactga
    25021 agccggaacc acgcaagagc tgccgaccaa ctccgggccc agcatgccgg atcttgcggg
    25081 ccctgcagag tacggcaacg tccatcccga ttgccattgc ggttcgcccc cgtcagggcc
    25141 tgacgattcc tgtctatcga aactgacgca ttgtgctgat gcgcccgtgg ccagcgaggt
    25201 gtaaattgcc ctcatccgcg ctttcatcga acaataatca ggagaatcgc atgaactatt
    25261 tccttaccgg cgggaccggt ttcatcggcc gcttcttcat cgagaacctg ctgcagcgac
    25321 aaggcaccat ccacgtgctc gtgcgcgaat cctcgcagca caagctcgag gagctgcggt
    25381 cgcgcttccc gctggcggcc gggcgcatcg ccgccgttac cggcgacatc acccggcccg
    25441 ggctcgggct ctgcgcggcg gacctcgagc ggctgaaggg ctcgatcgac cacttcttcc
    25501 acttcgccgc gatctacgac atggccgcca gcgaggcgag ccagaacgcc gccaacatca
    25561 gcggcaccct gcacgcggtg aacgccgccg cgcagctcgg cgcgggctgc ttccatcacg
    25621 tgagctcgat cgcggtgagc ggcctgtacc gcggcacctt ccgcgaggac atgttcgagg
    25681 aggccgagaa cctcgaccac ccctacttcc gcaccaagca cgagtcggaa aaggtggtac
    25741 gccagcagtg ccgcgtcccg taccggatct accgtccggc cgcggtggtc ggtcattcac
    25801 gcaccggaga gatcgacaag atcgacggcc cgtattactc gttcaagacc atccagcgca
    25861 tccgcaacgt gatgccgaag tggatgcccc tggtgggtgt cgagtccggc gtcaacaaca
    25921 tcgtaccggt cgactacgtc gtgaacgcga tcgactacct cgcgcacctc gagggccagg
    25981 acggcggctg cttccacatc gtcgacaccg agcaccactc caccggccag atgatgaaca
    26041 tcttcgccga ggccgcgcac gcgccgaagt tcagcgtgcg cttcgacccg aagatcttca
    26101 acttcatgcc cgcgacgctc gtcgacctgc tggccaagct gccgccggtc aagcgcttga
    26161 aggccgcggt cttcgagcgc atcggcatgc cggagtcggc ctccatcctg accaagtacg
    26221 ccacgaagta cgacagccgc cacacccggc gcctgctgga gggcagcggc atcgcgccgc
    26281 cgcgcctggc gagctatgcc ccgcgcatct gggactactg ggagcgcaac ctggaccccg
    26341 acctcttcgt cgatcgcacg ctggaaggcc aggtgaagga tcgcctggta ctgatcaccg
    26401 ggggctccgc gggcatcggg aaggccgccg cgatccgcct ggcgggcgcc ggcgccaggg
    26461 tggtgatcac cgcgcgcacg caggagaagc tcgacgagac ctgcgccgag atcgcgacac
    26521 agggcggcca ggccttcagc tacgtgtgcg acggcgcgga cctcgaatcg gtcgaccacg
    26581 tgatgcggca gatcgaagcc gatcacggcg gcgtcgacat cctgatcaac aacgccggcc
    26641 gctcgatccg ccgctcggtg gcgctggcct acgaccgctt ccacgatttc gagcgcacca
    26701 tgcaactcaa ttacttcggc gcgctgcgca tgatcctgcg cgtgctgccg ggcatggaca
    26761 agcggcgccg cggccacatc gtcaacatct cctcgatcgg cgtgctggga cacccaccgc
    26821 gcttctcggc ctatatcgcc tccaagtcgg cgctggaggc attctcgcgc tgcgccgcgg
    26881 cagaatattc cgaccgcaat atccacttca ccaatatcaa catgcctctg gtgcgcacgg
    26941 aaatgatcgc gcccaccaag atttacgagt acgcgccggt catggaggtg caggaggccg
    27001 tggacctgat cgtggaggcc atcatccaca ggaaggcacg cgtggcgacc gggatggggg
    27061 tgttcatgtc ggccatgggt gcgcttttcc cgaaggtcgc cgagatcgtg aacaatgcga
    27121 ccttccgcat gttcccggac tccgaggccg ccaagggcct gcctgccggc tcggccccgt
    27181 cggaggtgac cagcgagcag gtcgccatgg cacaattgat gaaaggcgta catttctgat
    27241 caccgtgcag gtcggcattc atgccgacgg gtgcaccgga aatggcgagc ccgattttca
    27301 ggagtcaacc cggatgagtt ccatcaagac cgaatacgac gccgccgttg cacggatcac
    27361 cgccccgggc gcccccttcg agctggtcaa tgccacgatc gacggtgtcg aataccgtct
    27421 ctatcgcaat gcgccacgca gccttgcgga ggtcttcgcg caggcgaccg cgcacggcga
    27481 gcgcgagttc ctggtctacg agggcgagcg ctggagctac acgcagttgc tggcgcaggg
    27541 ggcgcgcatc gcgcacgccc tgcgccggca ttgcggcatc cgcccgggtg accgcgtcgc
    27601 gatcgcgatg cgcaactacc ccgagtggat gagcgccttc atcggcatcg ccacgctggg
    27661 cgccgtcgcg gtgccgctga acagctgggg ccaggcgcgc gaactcgcgt tcgcgctcga
    27721 cgacgccggc gcgcgcgcgg ttttctgcga ccagcagcgc tgcgaccagc tcgctacgca
    27781 cctggccgcg accgggttgc cggccatcgt ggcgcgcccc acgggcgccg ggctgtctgg
    27841 cgggacgcgc agcctggagg acttcgttgc cgagatcgat gaaaccgcct ttccggccgt
    27901 ggatatcggg cccgaggagc ccgcgctgat gatgtacaca tcgggcacca cgggcacgcc
    27961 caagggcgcg ttgtcgacac agcgcgcggt gtgccagtcg ctgatgaact tcgactgcca
    28021 ggccatggcc tcggcgatga tcaatcccga agccataggt gcgatgatga aaaagggctt
    28081 cccgcccacg cagctgctcg cggtgccgct gttccatgtg agcggctgcc acgcggtgtt
    28141 cctgtgcgcg cttcgcggcg cgcggcgcgt ggtgatgatg tacaaatggg acgtgcgcaa
    28201 ggcgctggaa ctgatccagc gcgagcgcgt cacggcgctg agcgccgcgc cgggcatgct
    28261 gatggaactg atggagtcgc ccgacttcga gcgcttcgac acctcgagcc tgagcggcat
    28321 cggcggcggg ggcgcggcca cgcccccgcg cgccgcgcgc ctgttcatgg acaaggttgc
    28381 ggattcctac ccgggcgcgg gctgggggct gaccgagacc aattccaacg gcagcgcctg
    28441 caccggccgt ccgttccgcg agaagcccgg cacggccggc ttcctgcatc cgacggtgga
    28501 tctcagcatc cgcgacgaag agggcaacga actgccacag ggcgtgccgg gaaggctgtg
    28561 ggtcaggaca cccacgctgg tcagcggcta ctggaaccgc cccgaagcca acgagaccga
    28621 attccgcgac ggctggttcg acagcggtga catcgggtac ctcgatgacg acggctacct
    28681 gttcctgtcg gaccgtgcca aggacctggt catccgcggc ggcgagaaca tctatcccgc
    28741 cgagatcgag ggcgcgatcc tcgaatgccc cgaggtcgac gaggtcgccg cgttcggcgt
    28801 gcccgacgag acctggggcg agcagctcgc ggtggtggtg catgccacgc ccggcacgac
    28861 gatcagcgtc gaggacgtga agcgattcgc cgccgcgaag ctcgcgcact tcaaggtgcc
    28921 gcactacgtc gaggtgcgca gcgagcacct gccgcgcaac gccaccggca agatcctgaa
    28981 gaaggacatc cgcgccgagt tcctgcggaa gctctagacc gatcgggtga tgcgccggga
    29041 tttcccggcg cctgtcatcc gagccttttc gcatcaccaa ccccgggaga aacatcatgg
    29101 gtctgctcga caacaggatc gcactcgtca ccggcgccgg ccagggcgtg ggccagggca
    29161 tcgccttcgc gctcgcc
//
LOCUS       DAORUQ010000021        28962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1174_length_28962_cov_6.638600, whole genome shotgun sequence.
ACCESSION   DAORUQ010000021 DAORUQ010000000
VERSION     DAORUQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 28962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28962
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_28962_cov_6.638600"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..459)
                     /gene="gatC"
                     /locus_tag="PLW13_03445"
     CDS             complement(172..459)
                     /gene="gatC"
                     /locus_tag="PLW13_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQY69454.1"
                     /translation="MSISEQDMARVAELARIRLDDATVADVTRRIRDILAMIDQMQAV
                     NTDHVLPMANPLDATQRLRDDEVTEPDQREHFLAIAPATEDGLYLVPRVIE"
     gene            754..1791
                     /locus_tag="PLW13_03450"
     CDS             754..1791
                     /locus_tag="PLW13_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQY69455.1"
                     /translation="MFKRIRGLFSNDLSIDLGTANTLIYVRDQGIVLDEPSVVAIRVH
                     NGQKTIEAVGIEAKRMLGRTPGNITAIRPLKDGVIADFQVTEKMLQHFIAKAHQHSFI
                     RPSPRVLVCVPCKSTQVERRAIRESAYGAGARDVRLIEEPMAAAIGAGLSVEEASGSM
                     VVDIGGGTTEIAILSLNGVVYSDSVRIGGDRFDEAIVTYVRRTFGSLIGDATAERIKE
                     EIGCAMAGGEIREIDVRGRNLAEGVPRSFTLTSDEILEALQEPLNIIVQAVKGALEQS
                     PPELASDIAESGIVLTGGGALLRDIDRLIARETGLPVVVADDPLTCVARGGGKALEMM
                     DRHRLELLSSE"
     gene            1860..2834
                     /gene="mreC"
                     /locus_tag="PLW13_03455"
     CDS             1860..2834
                     /gene="mreC"
                     /locus_tag="PLW13_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQY69456.1"
                     /translation="MTGRNAIKPIFRSGPSISARVAALVIASVLLMLADWRFGALGPA
                     RVAFADAAVPFYWVTTLPGRLFGWAREVVVSRDTLIEENRVLRAEALVLRASTQRLAA
                     LAAENVRLRELLNSTALLDERVLVAEIIGVSPDMSSQTVVIDKGTAHGLAEGQAVIDA
                     YGLFGQIIEVSRFSSRVLLVTDSMHALPAQVVRNGVRVMVEGEGRIDVLDVNHVASTM
                     DVRPGDLLVSSGLGQRFPGGYPVGVVESVTNDPGKPFAVVVARPSAQLDRSRHVLVVT
                     EGEASVDAASSEAAPSGTAQEPVDAGVPGAAPRADAGAPAAPGQDGPP"
     gene            2831..3334
                     /gene="mreD"
                     /locus_tag="PLW13_03460"
     CDS             2831..3334
                     /gene="mreD"
                     /locus_tag="PLW13_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQY69457.1"
                     /translation="MRADEQGAPLPFAVAATGLAALLASLYPLPVWLQPARPNWLALV
                     VIFWILRLPQGFGIGSAWMLGLLMDGLEGGVLGRHALALSVVAYAAVLLRQRMLHYSL
                     LQQMLLVFALCATDQVLCHWVQNLAGHATSSLIFLMGSLTSAFCWPLFAFGAPRERSL
                     ESWRSSP"
     gene            3365..3979
                     /locus_tag="PLW13_03465"
     CDS             3365..3979
                     /locus_tag="PLW13_03465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQY69458.1"
                     /translation="MPTPAPDFYLASASPRRRELLAQLRYRFAALQVDIDETPQPGET
                     AAGLVLRLAGQKAAAGARLVALNDPRPVLGADTAVSVDGRILGKPATRDEAVAMLSLL
                     SGREHHVLTGVALLHAGRLDTAQSATRVVFRPVSVAEMQRYWDTGEPRDKAGGYAIQG
                     LAAAFVERIEGSYSGVVGLPLFETARLLQDSGVSGWQQDAPDHE"
     gene            3972..5450
                     /gene="rng"
                     /locus_tag="PLW13_03470"
     CDS             3972..5450
                     /gene="rng"
                     /locus_tag="PLW13_03470"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HQY69459.1"
                     /translation="MSEEILINSTPVQTRVALVESGVLQEIYVERSSHKGLVGNIYLG
                     RVMRVLPGMQAAFVDIGLERASFLHASDLVTLDANGVEVRSDETRDIRDLVCEGQSVV
                     VQVVKDPLGSKGARLTTHLSLSSRYVVFMPYTRHVGISQRIEQESERERLRTLVESIA
                     VAEGSAAPGGFIVRTAAEGVEREGIEGDMRFLVRLWGSLRERIALAKAPALVHEDLPL
                     AMRTLRDLVRPDLEKIRIDSTENFERLREFAGRFVPEAVERLELYQGDRPIFDLYSVE
                     DEIEKALGRRVQLKSGGYLIVDQTEAMTTIDVNTGAFVGSRNLEETIFKTNLEAATAL
                     ARQLRVRNLGGIIIIDFIDMQDPEHKRQVLRNLERAMERDHARTAISTVSELGLVQMT
                     RKRTRESLERTLCETCSTCQGRGTVKSAETVCYEIFREILREARSYENDTLLVLAAQS
                     VVDRLLDEESGNVADLEEFIGKTIRFQVETLYTQEQYDVVLL"
     gene            5499..9497
                     /locus_tag="PLW13_03475"
     CDS             5499..9497
                     /locus_tag="PLW13_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HQY69460.1"
                     /translation="MRRVLGRLLRWGWYLAAIVLVLCAALVTLGQYYFPYLGEHKDAL
                     LARVAADLPFGVEVAGLGTEWSGLAPTLHVRGLRLFARDEPEVTILSSSSSELRIDML
                     RSLFSLAPRLRRIVAEDVQLGFTEEADGRWHIAGVGGGRAPSNPEAIVDFFLAIEEID
                     LRRTRLVLRAHEGGRVETEGAELSMQNYRRFRRLQVTARDESPGGEFSLVLESHGDPR
                     QRDEFSAAAYVRLDSARLSRLQPLLPPALRMPEAALSGELWARLSEEGIVSLSGALRA
                     PELDPRAFWPERAQLQPLSDLDLRFSGQWAGNRGSLWLDELRCQWFGQPLELERVRID
                     AERAGGVNRIGVGAEYLDIGALAGALQSSTLLGGVWGEALAELSPYGGLVNAHLDLSL
                     PAASAPDFRFRAAMVDVSVSPRRQAPGVRNARGYLEITPRGGFVDIDSGAISLEFPRL
                     YRDDLEFDHVAGRVHWERDGGGLRVGSELLRAVTGATGISANFGLDLRKDPAADDTLS
                     LAFGLRDADALAYRTYLPYTVSPALREWLDASIEAGHVDSAAFTYHAVFSRPDRPFSY
                     AMQLGLDLRDAAVSYHPDWPEVSGAAGRVLVDERRVRVWAGSGRMLQSELRNATVEVL
                     PRRDERRLRLSAELDGDLADGLRVINTSPLAKTTGGALRSWQSSGAMALRLDLDMPFG
                     GRLAPETSRLDLAADIAGGRLFVADLRLPFDGVQGRLGFDLQRGLYSERLDGRLWGQP
                     LRATIGATEGPEHRIAIRVDGAAQLPRVAQWLGWDLDEYLAGVAPFSVELAQQPEHGF
                     GFVLRSSLAGAASTLPAPLAKAPADSLPLELRWSPHAAGTDISLQLGMDIWGLMQRDR
                     QGRFSGEIALGAEPVAGGEDLRIRGRVPQADAGAWIVAVQHVVAANAAREDGPRTVLR
                     LDGLELATATLLGAELHELRVDSRWAERDFVLDFVSDALSGEFRLPADPGARFALTLE
                     QLQLRAFLGPIAATPVAGEADAAGGASMARDAQAWRSLQDARLPETDVQVRRATLDDK
                     DLGEWTFRIGSEVDALAMTDVRATLPGLRLGGTGKDSGGSFRLRWIGGEPRSELVAGA
                     RSDDIERFFATWGYANVLQGRKGRADIEFSWPGNPAQFEMANVNGTLAFSWRDGRLLF
                     GGGDNPFMRTLGLMNFDQVLRRIRLDFKDLYQSGLAFDRFKGEIELGQGFAHTLEPIT
                     LEGPSARMKFSGRSDLRAQTIDADLIVTLPIGSNLPWVAALAGGLPAAAGVYVASRIF
                     EDQLGKFSSAIYKVSGKLDDPAVEFVKVFDTEDGQDDTSAAPARGESAPSAAPPARSP
                     DSATPAPASESP"
     gene            9503..10324
                     /locus_tag="PLW13_03480"
     CDS             9503..10324
                     /locus_tag="PLW13_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQY69461.1"
                     /translation="MRVAAVQMVSGEDIGHNLERARALIATAASGGARLVVLPEAFAC
                     YGSADAGALAGAERDSAGPLRSFLAATAREHGILLVGGTIPVAGEAPGARPHAACFLY
                     AEDGSELARYDKIHLFDVDLPDAQRRYRESDSYAPGERLVCAPTACGMLGLGVCYDLR
                     FPEMFRALGQRGMDVLALPSAFTRLTGEAHWHVLLRARAIENQVYVIAPDQGGRHSAT
                     RETWGGSVIIDPWGRVLASAASGEAVIAAEVDAAVLRDARERLPVRAHQRFYVPG"
     gene            complement(10309..10818)
                     /gene="yjgA"
                     /locus_tag="PLW13_03485"
     CDS             complement(10309..10818)
                     /gene="yjgA"
                     /locus_tag="PLW13_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HQY69462.1"
                     /translation="MSRKPQADDELPPSKSQRKREHRALQTLAEALIALPQSRFRRLA
                     LAERTREALEMARAMSASGARNRQVRLIAHLLEDEDIEALARPVTELSAQARAETALH
                     HRAERLRDVVLEQGPLALGFPLGAADSARLTELRTEALAPRDAARARHARREIYRVIL
                     ALLPDQPGT"
     gene            complement(10833..12191)
                     /gene="mgtE"
                     /locus_tag="PLW13_03490"
     CDS             complement(10833..12191)
                     /gene="mgtE"
                     /locus_tag="PLW13_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQY69463.1"
                     /translation="MTLASGRGSIRLRLEELLAAIDAGTLAPAKRLLNHLSAVDVAHL
                     LESSPPRARHVLWQLIDAEQEGEVLQELGDDVKGALLEQMDAIDVLALMEHLEPDDVA
                     DILQQLPGRIMQDVLRGMDAQDRRRVERVLSFEEDSAGGLMNTDTITVRPRHTVDVVL
                     RYLRRLGEIPHATDALFVVSRNDHFLGLLPVAKLLVCDPELTVREIMQTDVDPITVDM
                     PAREVAKLFRRHDWVSAAVVDSEGKLAGRITIDDVVDVIVEDADHSFMGMAGLGEDED
                     TFAPVLKATPRRAIWLGINLLTAFIASSVINLFEQTIDKVVALAVLMPIVASMGGVAG
                     SQTLTVVVRGMALGHIERSNAGWLLNRELMLAGLNGMLWALVCAAAAVLWFGDYLIGL
                     CIAAAMLINLLAAAAAGVLLPMLMRSLRIDPALAGSVVLTTITDAVGFLSFLGLATLF
                     YA"
     gene            complement(12188..12457)
                     /locus_tag="PLW13_03495"
     CDS             complement(12188..12457)
                     /locus_tag="PLW13_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQY69464.1"
                     /translation="MVSRELVIINRLGLHARAAAKLVSTAGRFSSTVRIERDGKGADA
                     KSIMAVMMLAAGKGTAVTVHTEGDDAVAALEAVAELINDRFGEDE"
     gene            complement(12460..13350)
                     /gene="rapZ"
                     /locus_tag="PLW13_03500"
     CDS             complement(12460..13350)
                     /gene="rapZ"
                     /locus_tag="PLW13_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQY69465.1"
                     /translation="MDGTPTPAKLVIVSGRSGSGKSTALHVLEDAGFYCIDNLPALLL
                     PELIEQARESDGITNKRMAVSIDARNMAGHLSRFGDILRMLPQDLPTEVIYLDADDAT
                     LIRRFSETRRKHPLSNRNTSLNEAIAAERMLLAAIAERAALTIDTSALSPHDLRELVG
                     RTVAVETDGSMVILFESFGFKRGIPIYADMVFDLRCLPNPHWNPALRRLSGLDEDVAK
                     FLQERPEVGEMLGDITAYLARWLPRFARSSRSYVTVALGCTGGQHRSVYMCEQLERHF
                     RDLFPGVQVRHRDLAGNRQN"
     gene            complement(13361..13834)
                     /locus_tag="PLW13_03505"
     CDS             complement(13361..13834)
                     /locus_tag="PLW13_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQY69466.1"
                     /translation="MTALDALLTPQRVLCGADCGSKKRVLETIAGLIAGGRGKEIEDQ
                     LCESLNARERLGSTGIGEGIAIPHCREADCSAPMAALVTLARPVDFAAIDDNPVDIVF
                     ALVVPDGATDEHLRVLARLAGLFSRPGVRERLRACRDDESLRATMLAAWAQGDEA"
     gene            complement(13858..14166)
                     /gene="raiA"
                     /locus_tag="PLW13_03510"
     CDS             complement(13858..14166)
                     /gene="raiA"
                     /locus_tag="PLW13_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQY69467.1"
                     /translation="MQINITGHHVDLTDALRDYVASKLGRLERHESRITHADVVLNVE
                     KLRQKVEATIRMAGAELFAAAESEDMYAAIDALTDKLDRQLNKHKGKSQDHHQGLAPR
                     "
     gene            complement(14241..15725)
                     /locus_tag="PLW13_03515"
     CDS             complement(14241..15725)
                     /locus_tag="PLW13_03515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQY69468.1"
                     /translation="MKQSLQLKLGQHLTMTPQLQQAIRLLQLSTLDLQQEIQEALDSN
                     PLLELVESTPADDVVPGMASSQQELPEEYAGSAEREVAEPDTVDDVTAESMPSDLAVD
                     TSWDDIYETGSAGPAGSGDEGQDFEQYTAEASTLSDHLLWQLNLTPMSETDRMIAMSI
                     IDAIDADGMLSVSLEDLAEGFGPESGIEIDEIVAVLHRIQHFDPVGVGARDLRECLLI
                     QLRQLDPEEPAVRHARVIIDKHIQLLGAKDYATLMRRLHISEPELKAAIHCIQQLNPR
                     PGSVISASAAEYVVPDVIVSKRNGRWLVELNPEIAPRLRVNSYYAAMIRRADNSADNV
                     FLRDNLQEAKWFIKSLQSRNETLLKVARCIVERQQGFLEHGPEAMRPMILHDVATAVS
                     MHESTISRVTTQKYMHTPGGIFELKYFFSSHVSTAAGGECSSVAIRAIIKKLVSAEHP
                     RKPLSDSKIASILEQQGINVARRTIAKYREALGIAPSNERKQLV"
     gene            complement(15838..16563)
                     /gene="lptB"
                     /locus_tag="PLW13_03520"
     CDS             complement(15838..16563)
                     /gene="lptB"
                     /locus_tag="PLW13_03520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQY69469.1"
                     /translation="MARLEALNLAKSYRSRKVIRDVSISMDSGEIVGLLGPNGAGKTT
                     CFYMILGIVPADSGRILLGGEDITRLPMYGRARKGIGYLPQEASVFRRLTVAQNILAI
                     LQTRKTLSRQRMQEKLEELLQEFNLTHLSDARGIALSGGERRRVEIARALASDPSFIL
                     LDEPFAGVDPISVKDIKDIVRHLQARGIGVLITDHNVRETLDICERAYIVGEGHIITE
                     GDAATILANDKAREIYLGQQFRM"
     gene            complement(16550..17164)
                     /gene="lptA"
                     /locus_tag="PLW13_03525"
     CDS             complement(16550..17164)
                     /gene="lptA"
                     /locus_tag="PLW13_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HQY69470.1"
                     /translation="MGAETGVLRIGRLRLRTIAARAAALVLWGVLAPRPGLALPDDRN
                     QPIYIQSDRAERDERKGITVYTGDVEIDQGSLHISADRVTIAMDGDQVTRIDALGAPA
                     KMHQKPSPEREPVYARATTIQYDVAREILTLIEKAAVTQEGSTVKGERIEYFVQEQRV
                     KASSGSAESGASRVQMVIQPRRATLAPPASDGQPPAEASPDGAP"
     gene            complement(17192..17752)
                     /locus_tag="PLW13_03530"
     CDS             complement(17192..17752)
                     /locus_tag="PLW13_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQY69471.1"
                     /translation="MTEELKAIARRIRLLALDVDGVLTDGRIHYSGDGEEMKSFSILD
                     GLGIKLVRRCGIQVAIITARRSGAVERRARELGIDHCVQGREDKLEALRELLASTGLT
                     LAETAYMGDDLPDLRAILAVGLGMTVANASSEIARRAAWQSRARGGEGAVREACEMLL
                     RAREQWTEALAPYLPEAGGTAGHRGL"
     gene            complement(17778..18767)
                     /locus_tag="PLW13_03535"
     CDS             complement(17778..18767)
                     /locus_tag="PLW13_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003188589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQY69472.1"
                     /translation="MAADNASPSSPWLQSAQRTVALEIEAVTRLRERLDESFVRACEI
                     LLGCAGRVVVTGMGKSGHIGNKIAATLASTGTPAFYVHPGEASHGDLGMITGKDVVLA
                     LSNSGTTPEILTILPLIKRMGAPLIALTGKPESTLARLADVNLDVGVETEACPLDLAP
                     TSSTTVALVMGDALAIALLEARGFSAEDFAFSHPGGALGRKLLLRVNDIMHQGREVPR
                     VPPETVIREALLEMTAKGFGMTTVTDPGGHLLGIFTDGDLRRALDRGIDFQHERIGDF
                     MTTRCKTVNGEALAAEALHLIEESKITSLVVLDDERRPTGVIHLHDLLRAGIA"
     gene            19029..19268
                     /locus_tag="PLW13_03540"
     CDS             19029..19268
                     /locus_tag="PLW13_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HQY69473.1"
                     /translation="MTEDEIRALVMAGMPGADVAVGGDGYHIDLTVVSDAFEGLNRVK
                     RQQLVYGVLADVIRSGALHAVNIRALTAAESQATI"
     gene            19286..20548
                     /gene="murA"
                     /locus_tag="PLW13_03545"
     CDS             19286..20548
                     /gene="murA"
                     /locus_tag="PLW13_03545"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQY69474.1"
                     /translation="MEKFSIHGGRRLDGEIRISGAKNAALPILAATLLTDDTLRIRNV
                     PDLQDITTMIDLLASMGVVLRRDERHGLEADGANIGSFTAPYELVKTMRASILVLGPM
                     LARFGQAQVSFPGGCAIGSRPIDLHLHGLQAMGAEIEIDGGYINARVQGRLRGAHVLF
                     DKVTVGGTENLLMAACLADGRTVLENAAREPEIVDLARCLQRMGAQIAGAGTDTLVIE
                     GVERLHGCDYTVMPDRIETGTFLVAAAATRGRVKLRDTRADILEAVLVKLTEAGAHIE
                     EGEDWIALDMQGRRPRAVSIRTAPYPAFPTDMQAQFTAMNAVAEGSSTMTETIFENRL
                     IQVHEMNRMGAHIVVEGHTAIVEGVSALRGAPVMATDLRASASLVIAGLVAEGETVVE
                     RIYHIDRGYECIEEKLQMLGADIRRVRA"
     gene            20545..21231
                     /gene="hisG"
                     /locus_tag="PLW13_03550"
     CDS             20545..21231
                     /gene="hisG"
                     /locus_tag="PLW13_03550"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQY69475.1"
                     /translation="MSASAGTPLTLALTRGRILKDCLPLLAAAGIVPEEDPGSSRKLV
                     IGTNRADVRLLVLRGSDVSVYVRHGAADAGVVGRDMLLEAGSEGLYEPLDLGIARCRL
                     MTAGLVERAPLRSRVRVATKFVNVARRFYAAQGLQADIIKLYGAMELAPVLGLAERIV
                     DIVDTGNTLRANGLEPIETIAEISSRLVVNKAAMKLRPGPLGELIEGLAAAVGQRDAA
                     APRGGAGGAP"
     gene            21228..22538
                     /gene="hisD"
                     /locus_tag="PLW13_03555"
     CDS             21228..22538
                     /gene="hisD"
                     /locus_tag="PLW13_03555"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQY69476.1"
                     /translation="MMRRLDASAPGFAGELAALLDFSAQRDAGVVEQVAAIIADVRAR
                     GDAALLEYTNRFDARALVEATGLEVAPAALAQASARIPAQLGEALQAAASRIESYHLR
                     QRQESWQYRDADGTLLGQRITALDRAGLYVPGGKASYPSSVLMNAIPARVAGVGELVM
                     VVPAPRGELSDAVLAAAHIARVDRVFTIGGAQAVAALAFGTATVPRVDKIVGPGNVYV
                     AEAKRQVFGEVGIDMIAGPSEIAVICDGATDPEWIALDLFSQAEHDEHAQSILISPDA
                     AFLDAVANRIARLLPTMARADIIGRSLAARGAFILVRDLAEAAEISNRIAPEHLELSV
                     AEPEQLLAAIRHAGAVFLGRHTPEAIGDYCAGPSHVLPTAGTARFASPLGVHDFQKRS
                     SLIGCSPGGADRLGQLARVLAESESLQAHALSAAARVRKPGEGA"
     gene            22535..23635
                     /gene="hisC"
                     /locus_tag="PLW13_03560"
     CDS             22535..23635
                     /gene="hisC"
                     /locus_tag="PLW13_03560"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQY69477.1"
                     /translation="MTRRDAADWVNALVRADVRATAAYHVADARGMVKLDAMENPFAL
                     PAQLQSLLGERVATAALNRYPDPQARALSASIRSTFGIDPRWGILLGNGSDEIIQMLI
                     AGVSGPGCCVLAPAPSFVMFRLISQWLNVRFEEVTLRSDFQLDVPAMREAIRLHRPRL
                     IFLACPNNPTGNLFDEASLRAVLEAADALVVIDEAYLAFASRDHTALLDEYPNLLIMR
                     TLSKLGLAALRLGYLVGDPRWIAEFDKIRLPYNIGTLNQVAAEVALEHFDVLDAQTRL
                     IVSERARLLVRLQADARLHCWQSEANFLLVRPHAASARTVHAGMARRGVLVKCLDGGH
                     PLLANCLRLTIGAPPENDAMLAALDAELAVQS"
     gene            complement(23626..24756)
                     /locus_tag="PLW13_03565"
     CDS             complement(23626..24756)
                     /locus_tag="PLW13_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY69478.1"
                     /translation="MAETPGLNGWRAMGWPVATGLMAALLILQTLHYRELAGRPTTST
                     EAASVDAPTSYAAAVRRATPAVVNIYTRKVVRRPYSPLFDEPFFQRLLNRGTAPQRER
                     IQRSLGSGVIMSGDGYLLTNNHVIEGANEILVLLADGRDAIAKVIGTDPDTDLAVLDI
                     DLPQLQPIPVGNPDAAEVGDVVLAIGNPYGFGQSVSQGIISATGRFGLQLNTYENYIQ
                     TDAAINPGNSGGALLDARGNLLGINTAIYSRSGGSQGIGLAIPADYAIKVLRDIVAHG
                     YVVRGWLGVEVQPLPARIVTADGAHTGLVVSEVEPGSPAQTAGVQAGDILLALNGTAT
                     SQGRNAMYRIALMAPGDAFELEIYRGEKLTRLAGTLGQRPQD"
     gene            complement(24784..25236)
                     /locus_tag="PLW13_03570"
     CDS             complement(24784..25236)
                     /locus_tag="PLW13_03570"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HQY69479.1"
                     /translation="MYSSAVFWITVVACLLAGVAAGWLIRRQFDPAEQRQRDLERRLL
                     ENELALRDYKSQVTAHFKGTAERVNRLTEDYRELHQHLAEGALGLCEPRQPGDEPPLL
                     TSLGGPGYRSAASVPPSVNPPLDYAPRGSTQQPGMLNEEYDLERVHAG"
     gene            25390..26481
                     /gene="zapE"
                     /locus_tag="PLW13_03575"
     CDS             25390..26481
                     /gene="zapE"
                     /locus_tag="PLW13_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HQY69480.1"
                     /translation="MGPAERYRAELGRPGFVRDPAQERAVGELQRVYEELTARPARGR
                     MRRLVSRVAGDSREPVMGLYLWGGVGRGKTHLMDLFYASIPFDEKLRTHFHRFMQRVH
                     RELRDCAGEKNPLRLIGKRIAREARLLCFDEFFVADITDAMLLAGLFEELFARGVTLV
                     ATSNVEPDDLYRDGLQRARFLPAIAMIKRHTRVINVDGGIDHRLRRLEQAQLYHCPLG
                     DRADESLMRSFTSLAPEEPVRERVLEVEGRPIRTRFCADDVVWFDFAELCDGPRSQND
                     YIELAREFHAVLVGGVPVFTADKDDQARRFINLVDEFYDRNVKLVLSAAAPLAELYAG
                     GRLGFEFQRTVSRLMEMQSHEYLARAHRA"
     gene            26585..27013
                     /gene="rplM"
                     /locus_tag="PLW13_03580"
     CDS             26585..27013
                     /gene="rplM"
                     /locus_tag="PLW13_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQY69481.1"
                     /translation="MKTISAKPGSVEQSWYVIDANGQTLGRMASAIASRLRGKHKPEF
                     TPHTDTGDFIVVVNAEKVHVTGNKAKAKIYHHHTGYPGGLKSISFEKLIEKAPEQAIE
                     MAVKGMLPKNPLGRAMARKLKVYAGSEHPHAAQQPKALEL"
     gene            27031..27423
                     /gene="rpsI"
                     /locus_tag="PLW13_03585"
     CDS             27031..27423
                     /gene="rpsI"
                     /locus_tag="PLW13_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQY69482.1"
                     /translation="MAATQYYGTGRRKTATARVFLRQGRGAISINDRPLDQYFGRQVA
                     RMIVRQPLELVGLGEKFDIHVSVAGGGSFGQAGAIRHGITRALIEYDETLKSALRQQG
                     YVTRDAREVERKKVGLRKARKRPQYSKR"
     gene            27661..28263
                     /gene="petA"
                     /locus_tag="PLW13_03590"
     CDS             27661..28263
                     /gene="petA"
                     /locus_tag="PLW13_03590"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQY69483.1"
                     /translation="MSKKGVNKGRRQFLTAATSVVGAAGVVGVAVPFLSSWNPSAKAK
                     AAGAPVKADISKLEPGQMIVVEWRGKPVYVVRRTQESLDALTTGSLVEVLRDADSAES
                     AQPAYAGGVQRSIKPEFLVLVGLCTHLGCAPKYRPDVGATDLGGDQWLGGFFCPCHGS
                     KFDLAGRVFKGVPAPLNLEVPPHMYEGDKVLVIGVDQETA"
     gene            28263..>28962
                     /locus_tag="PLW13_03595"
     CDS             28263..>28962
                     /locus_tag="PLW13_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HQY69484.1"
                     /translation="MNWLVGLRDWVDARLPIVRAWDTHMGKYYAPKNFNFWYFFGVLS
                     LLVLVNQLLTGVWLTMSFEPSAERAFAAVEYIMRDVEYGWLIRYMHSTGASAFFVVVY
                     LHMFRGLLYGSYRKPRELVWIFGMFIYFMLMAEGFLGYVLPWGQMSYWGAQVIISLFG
                     AIPYFGEDLVQWIRGDYLISGITLNRFFALHVVAVPIILLALVVLHLLALHEVGSNNP
                     DGVEIKKKKDANGVP"
BASE COUNT         4222 a         9539 c        10320 g         4881 t
ORIGIN      
        1 gatgtaggca ttcagggtcg ggttcatccg ggcgatgcgc gcgaggaagt gcgccgtgat
       61 ctccgccgag gagaactccc ggcgcgcgag tgcatcgcgg atggcgtgga gggaaaggtc
      121 gtgcatgcgg ggcgtttcct gggcgttctg ggggcgcgcg gtgccggttt cctactcgat
      181 gacccgcggc acgaggtaga ggccgtcctc ggtcgccggt gcgatcgcga ggaagtgctc
      241 gcgctggtcg ggttcggtga cctcgtcgtc acgcaggcgc tgggtggcgt ccagcgggtt
      301 ggccatcggc agcacgtggt cggtattcac ggcctgcatc tggtcgatca tcgcgaggat
      361 gtcccggatg cggcgggtga cgtcggccac ggtggcgtcg tcgaggcgga tgcgtgccag
      421 ttcggcgacg cgggccatgt cctgttcact gatcgacatc tgcaatggtc caaatacggc
      481 aggagccagg gcggcgggcc ctggccgctg gcgcgtgttg ccgggtggat taccctgaaa
      541 tttcattcgc cgtcggggcg tcagggggcg taaacttagc acattccagc cttgcccaga
      601 atacgcacgg ttgttagatt gaccggtttg aaaccgtgcc gtctgcgccg gcacttccag
      661 cctgccgggt tcgcctgttg cgccgcggcc cggcaccacc gccggcatcc ggtgcgcacg
      721 ggcgttgctc catccgaggt ttagacacgt agtatgttca agagaatccg cggacttttc
      781 tccaatgacc tgtcgataga cctgggaacc gccaacacgc tgatctacgt gcgcgaccag
      841 ggcatcgtgc tggacgagcc ctcggtggtc gcgatccggg tccacaacgg ccagaagacc
      901 atcgaggcgg tgggaatcga ggccaagcgc atgctgggcc gcacgccggg caacatcacc
      961 gcgatccgcc cgctgaagga cggggtgatc gccgacttcc aggtcaccga gaagatgctg
     1021 cagcacttca ttgccaaggc gcaccagcac agcttcatcc gccccagtcc gcgcgtgctg
     1081 gtgtgcgtgc cctgcaaatc cacccaggtc gagcgtcgcg ccatccgcga gtcggcttac
     1141 ggtgccgggg cacgcgacgt gcgcctgatc gaggagccga tggcggcggc gatcggcgcc
     1201 gggctctcgg tcgaggaggc cagcggttcg atggtcgtcg acatcggtgg cggcaccacg
     1261 gagatcgcga tcctgtcgct caacggcgtg gtgtactcgg actcggtgcg catcggcggc
     1321 gaccgcttcg acgaggccat cgtgacctat gtgcggcgca ccttcggcag cctgatcggc
     1381 gacgcgacgg ccgagcgcat caaggaggag atcggctgcg ccatggccgg cggcgaaatc
     1441 cgcgagatcg acgtgcgcgg gcgcaacctc gccgagggcg tgccacgttc gttcacgctg
     1501 accagcgacg agatcctcga ggcgctgcag gagccgctga acatcatcgt gcaggccgtc
     1561 aagggcgcgc tcgagcagtc accgcccgaa ctggcatcgg acattgccga gagcggcatc
     1621 gtgctgaccg gcggcggtgc gctgctgcgc gacatcgacc gcctgatcgc gcgcgagacc
     1681 ggcctgccgg tcgtggtcgc agacgatccg ctgacctgcg tggcccgcgg cggcggcaag
     1741 gcgctcgaga tgatggatcg ccatcgcctg gaactgctgt cgtcggagtg atccgcgtgg
     1801 cgtgagcagg cccggcgacc gacccggccg ggcgggaccg gatccgagca ttgccggtgg
     1861 tgacggggag aaacgcgatc aagcccatat tccgcagcgg accgtcgata agcgcgcgtg
     1921 ttgcggcgct ggtgattgcg tccgtgctgc tgatgctcgc ggactggcgc tttggcgcgc
     1981 tcgggccggc gcgcgtcgcg tttgccgatg ctgccgtgcc gttctactgg gtgaccacgc
     2041 tgcccggccg actgttcggc tgggcgcgcg aagtggtcgt ttcccgcgac accctgatcg
     2101 aggagaaccg cgtgctgcgg gccgaggcgc tcgtgctgcg cgccagtacc cagcgtctcg
     2161 ccgcgctcgc ggcggagaac gtgcgcctgc gcgagttgct gaattccacg gcgctgctcg
     2221 acgagcgcgt gctcgtcgcc gagatcatcg gcgtctcgcc cgacatgtcc agccagaccg
     2281 tggtgatcga caagggcact gcccacggcc tcgccgaggg acaggcggtg atcgatgcct
     2341 acggcctgtt cgggcagatc atcgaggtca gccgtttcag cagccgcgtg ctgctcgtca
     2401 ccgattccat gcatgcgttg cccgcgcagg tggtgcgcaa cggcgtccgg gtgatggtcg
     2461 agggagaggg tcgtatcgat gtcctcgacg tcaaccatgt cgcctcgacg atggacgtgc
     2521 gccccgggga tctcctggtc agctccgggc tggggcagcg gtttccggga ggctatccgg
     2581 tcggcgtggt cgagtcggtg accaacgatc ccggcaaacc cttcgcggtg gtggtggcgc
     2641 gtcccagcgc acaactcgat cgcagccgtc acgtgctggt cgtcaccgag ggcgaggcga
     2701 gcgttgacgc cgcgtcttcc gaagccgcgc cgtccgggac cgcgcaggag ccggtggatg
     2761 ccggagtgcc gggggcggcg ccgcgagccg atgctggcgc gccggccgcg cccggccagg
     2821 acggcccccc gtgagggccg acgagcaggg cgccccgcta ccgttcgcgg tggcggcgac
     2881 cgggctggcc gcgctgctcg cctcgctgta tccgctgccg gtgtggctgc agccggcgcg
     2941 gccgaattgg ctcgcgctgg tggtgatctt ctggatcctg cgcctgcccc agggtttcgg
     3001 catcggatcg gcctggatgc tgggactgct gatggacggt ctcgagggcg gtgtgctcgg
     3061 gcgccacgcg ctcgcgctgt ccgttgtcgc ttacgcagcc gtcctgctgc ggcaaaggat
     3121 gctgcactac agcctgctgc agcagatgct gctggtgttt gcgctgtgcg caacggacca
     3181 ggttctgtgt cactgggtgc agaacctggc cggccacgcg acctcgagcc tgatctttct
     3241 gatgggcagc ctgaccagtg ccttctgctg gccgctgttc gcgttcggcg cgccccgcga
     3301 gcgctccctc gaaagctgga ggtcgtcgcc ctgagcccgc ggcgcgggcg caacgaacta
     3361 ccatatgccg acaccagccc ccgatttcta tctcgcctcc gcttcgccgc gacgccgcga
     3421 gttgctggcg cagttgcgct accgtttcgc cgcgctgcag gtggacatcg acgagacgcc
     3481 gcagccgggc gagaccgcgg ccgggctggt gctgcgcctg gcggggcaga aggccgccgc
     3541 gggcgcgcgc ctggtggcgc tcaatgaccc gcggccggtg ctcggcgctg acacggccgt
     3601 aagcgtcgat gggcggattc tcggcaagcc cgccacccgc gacgaggcgg tagcgatgct
     3661 gtcgctgctg tcggggcgtg aacaccacgt gctgaccggc gtggcactgc tgcatgcggg
     3721 gcgtctcgat accgcgcaga gcgcgacccg cgtggtgttc cggccggtct cggtcgcgga
     3781 gatgcagcgc tactgggata ccggcgagcc gcgcgacaag gccggtggct acgcgatcca
     3841 ggggctggcg gcggccttcg tggagcgtat cgagggcagt tattccggcg tcgtcggcct
     3901 gccccttttc gagaccgcgc gcttgctgca ggattcgggt gtcagcggct ggcaacagga
     3961 tgcgccggac catgagtgaa gagatactga tcaactccac gccggtgcag acccgcgtcg
     4021 cgctcgtcga gagcggcgtg ctgcaggaaa tctacgtcga gcgcagcagc cacaaggggc
     4081 tggtcggcaa catctacctc gggcgcgtga tgcgcgtgct gccgggcatg caggccgcct
     4141 tcgtcgacat cggcctggag cgtgcgagtt tcctgcatgc gagcgacctg gtcacgctgg
     4201 acgcgaacgg cgtcgaggtg cgcagcgacg agacgcgcga catccgcgac ctggtgtgcg
     4261 agggccagtc ggtggtggtg caggtggtga aggatccgct gggcagcaag ggcgcgcggc
     4321 tcaccacgca tctgtcgctg tcttcgcgct acgtcgtgtt catgccgtac acgcgccatg
     4381 tcggcatttc gcagcgcatc gagcaggagt ccgagcgcga gcgcctgcgc acgctggtcg
     4441 agagcatcgc cgtcgccgag ggcagcgccg cgcccggcgg tttcatcgtg cgcaccgcgg
     4501 ccgagggcgt ggagcgcgag ggcatcgagg gcgacatgcg cttcctggtg cggttgtggg
     4561 gttcgctgcg cgagcgcatc gcgctggcca aggcgccggc cctggtgcac gaggatctgc
     4621 cgctggccat gcgcacgctg cgtgacctcg tgcgccccga tctcgagaaa atccgcatcg
     4681 actcgacgga aaatttcgag cgcctgcgtg aattcgcggg ccggttcgta ccggaagcgg
     4741 tcgagcggct cgagctgtac cagggagacc ggcctatctt cgacctctac agcgtggagg
     4801 acgagatcga gaaggcgctg ggccggcggg tgcagttgaa gtccggcggc tacctgatcg
     4861 tggaccagac cgaggcgatg accacgatcg acgtcaacac gggcgccttc gtcggcagcc
     4921 gcaacctcga ggagacgatc ttcaagacca acctcgaggc ggcgaccgcg ctcgcgcgcc
     4981 aactgcgcgt gcgcaacctc ggcggcatca tcatcatcga cttcatcgac atgcaggatc
     5041 ccgagcacaa gcgccaggtg ctgcgcaacc tcgagcgcgc gatggagcgt gaccacgcgc
     5101 gcacggcgat cagcacggtc tcggagctcg gcctggtgca gatgacgcgc aagcgcacgc
     5161 gcgagagcct ggaacgcaca ctgtgcgaga cctgttcgac ctgccagggc cgtggcacgg
     5221 tgaagtccgc cgagacggtg tgctacgaaa tcttccgcga gatcctgcgc gaggcgcgca
     5281 gctacgagaa cgacacgctg ctggtgctcg ccgcgcagtc tgtcgtcgac cgcctgctgg
     5341 acgaggagtc cggcaacgtc gcggacctcg aggagttcat cggcaagacc atccgcttcc
     5401 aggtcgagac gctgtacacc caggagcaat atgacgtcgt gctgctctga gagtccggca
     5461 gcattgcgcc ggcctgccgc ggccggggtg gcgcggcgat gaggcgcgtg ctgggccggc
     5521 tcctgcgctg gggctggtat ctcgcggcca tcgtgctggt gctgtgcgcc gcactggtga
     5581 cgctcgggca gtactatttt ccctacctcg gcgagcacaa ggacgcgctg ctggcgcgtg
     5641 tcgccgcgga cctgccgttt ggcgtcgaag tggcggggct cggcacggag tggagcggtc
     5701 ttgcacccac gctgcacgtg cggggcctgc gcctgttcgc gcgcgacgag cccgaggtga
     5761 cgatcctctc ctccagcagc tcggagctgc gcatcgacat gctgcgctcg ctgttcagcc
     5821 tggcgccgcg gttgcgccgc atcgtcgccg aggacgtgca gctcggcttc accgaggagg
     5881 ccgacggcag gtggcatatc gccggcgtgg gcggaggccg cgcgccgtcc aatcccgagg
     5941 cgatcgtcga tttcttcctc gccatcgagg agatcgatct gcggcgaacg cgcctggtat
     6001 tgcgcgccca cgagggcggg cgcgtcgaga ccgagggtgc cgagctgtcg atgcagaact
     6061 accgccgctt ccgccgcctg caggtgacgg cgcgtgacga gtcgcccgga ggagagttca
     6121 gcctggtgct ggaatcgcac ggcgacccgc ggcagcgcga cgaattctcc gctgccgcct
     6181 acgtgcgtct ggactctgcg cgactgtcgc ggttgcagcc gctgctgccg cccgcgctgc
     6241 gcatgcccga ggcggcgctg agcggagagc tctgggcgcg cctgtcggag gaggggatcg
     6301 tgagcctgag cggcgcgctg cgtgctccgg agctcgaccc gcgcgcgttc tggcccgagc
     6361 gcgcgcagtt gcagccgctg agcgatctcg acctgcgctt cagcgggcag tgggccggga
     6421 accgcggctc gctgtggctc gacgagctgc gctgccagtg gttcgggcag ccgctcgagc
     6481 tcgagcgcgt gcgcatcgac gccgagcgtg ccgggggcgt gaaccggata ggggtgggcg
     6541 cggagtatct cgacatcggg gccctggccg gcgcgctgca gagctcgaca ctgctcgggg
     6601 gggtgtgggg cgaggcgctc gccgagctct cgccgtacgg cgggctggtc aatgcgcacc
     6661 tcgacctgtc gctgcccgcc gcgtcggcgc ccgatttccg tttccgtgcc gccatggtcg
     6721 acgtctcggt ctcgccgagg cgacaggcgc cgggcgtgcg caatgcgcgc ggttacctgg
     6781 agatcactcc gcgtgggggc ttcgtggata tcgacagcgg tgcgatctcg ctggaattcc
     6841 cgcggctcta ccgcgatgac ctcgagttcg atcacgtcgc cgggcgcgtg cactgggagc
     6901 gcgacggcgg cggactgcgg gtcggcagcg agctgctgcg ggcggtgacg ggcgccaccg
     6961 ggatcagcgc gaatttcggc ctcgatttgc gcaaggatcc ggcggccgac gacaccctct
     7021 cgctcgcgtt cggcctgcgt gacgccgatg cccttgccta ccgcacctat ctgccataca
     7081 cggtgtcacc ggcgttgcgc gagtggctcg atgccagcat cgaggccggt catgtcgaca
     7141 gcgcggcctt tacctatcac gcggtattca gccgtccgga tcgtccgttc tcgtatgcga
     7201 tgcagctcgg gctcgacctg cgcgacgccg cggtgtccta tcaccccgac tggccggagg
     7261 tgagcggggc ggcggggcgc gtcctggtcg acgaacggcg tgtgcgcgtg tgggccggtt
     7321 ccgggcgcat gctgcagagc gagctgcgca acgcgacggt cgaggtgctg ccgcgacgcg
     7381 acgagcgtcg cttgcgcctc agtgccgagc tcgacggcga tctcgccgac ggcctgcggg
     7441 tcatcaatac ctcgccgctg gcgaagacga cgggtggcgc gctgcgcagc tggcagtcca
     7501 gcggcgccat ggcgctgcgg ctcgatctcg acatgccctt cggcgggcgg ctggcgccgg
     7561 agacctcgcg cctcgatctg gccgccgaca tcgcgggcgg gcgcctcttt gtcgccgacc
     7621 tgcggctgcc cttcgacggc gtgcaggggc ggctcggttt cgacctgcag cgcggccttt
     7681 acagcgagcg cctggacggc aggctgtggg ggcagccgct gcgcgcgacc atcggtgcca
     7741 ccgagggtcc ggagcaccgc atcgcgatcc gggtagacgg cgccgcgcag ttgccgcgcg
     7801 tggcccaatg gctgggctgg gacctcgacg agtaccttgc cggcgtggcg cccttcagcg
     7861 tggagcttgc gcaacaaccg gagcacggct tcggcttcgt gctgcgatcg tcgctcgcgg
     7921 gcgcggccag cacgctgccg gcgccgctcg cgaaggcgcc cgccgattcg ctgccgctcg
     7981 agctgcgctg gtcgccgcat gccgcgggca cggatatcag cctgcagctc ggcatggata
     8041 tctgggggct catgcaacgt gaccggcagg gacgcttcag cggcgagatc gcgctgggcg
     8101 ccgagccggt ggcgggcggc gaggacctgc gcatcagggg gagggtgccg caggccgatg
     8161 ccggggcttg gatcgtggcc gtgcagcacg tcgtggccgc gaatgccgcg cgcgaggacg
     8221 ggccgcgcac cgtgctgcgt ctggacgggc tggaactggc aacggccacg ctgctcggcg
     8281 cagagttgca cgagctgcgg gtcgattcgc gctgggcgga gcgcgatttc gtgctggatt
     8341 tcgtttccga tgcgctgagc ggcgagttcc gcctgcccgc cgatcccggc gcgcgctttg
     8401 cgctgacgct cgaacaactt cagctacgcg ctttcctggg gcccatcgcg gcgacgccgg
     8461 tcgccgggga ggcggacgct gccggcggcg cgtcgatggc ccgggacgcg caggcctggc
     8521 gcagcctgca ggatgcccgc ctgcccgaga ccgatgtgca ggtgcgccgc gccactctcg
     8581 acgacaagga cctcggcgag tggacgttcc gcatcgggtc cgaggtcgat gcgctggcga
     8641 tgacggacgt gcgtgccacg cttcccgggc tgcgtctcgg cggcaccggc aaggacagcg
     8701 gtggcagctt ccgcctgcgc tggatcggcg gcgagccgcg cagcgaactc gtcgcggggg
     8761 cgcgcagtga cgacatcgag cgttttttcg cgacctgggg ttatgccaac gtgctgcaag
     8821 ggcgcaaggg cagggccgat atcgagttca gctggcccgg caacccggcg cagttcgaga
     8881 tggcgaacgt caacggcacg ctggccttct cctggcggga tggccgtctg ctgttcgggg
     8941 gcggcgacaa tccgttcatg cgcacgctcg ggctgatgaa tttcgaccag gtgctgcgac
     9001 gcatccggct cgatttcaag gacctctacc agagtggcct ggccttcgac cgcttcaagg
     9061 gtgagatcga gctcgggcag ggtttcgcgc acaccctcga gccgatcacg ctggaagggc
     9121 cttcggcgcg catgaagttc tcgggccgtt ccgacctgcg ggcgcagacc atcgacgccg
     9181 atctcatcgt gacgctgccg atcggcagca acctgccgtg ggttgcggcg ctcgccgggg
     9241 gcttgcccgc ggccgcgggc gtctacgtcg caagccgcat cttcgaggat cagctcggca
     9301 agttttccag cgcgatctac aaggtgagcg gcaagctcga cgatccggcg gtcgagttcg
     9361 tgaaagtgtt cgataccgag gacgggcagg acgacacgag tgccgcgccg gcgcgcggcg
     9421 aatccgcgcc ctccgcggcg ccgcccgcgc gatcccccga tagcgcaacg ccggcgccgg
     9481 cgtcggagtc gccgtgagcg gcgtgcgcgt cgccgccgtg cagatggtga gcggcgagga
     9541 catcgggcac aacctggagc gtgcgcgcgc gctgatcgcc accgcggcct ccggcggcgc
     9601 gcgcctggtg gtcctgcccg aggcttttgc gtgctacggc agcgccgacg cgggcgcact
     9661 cgccggcgcc gagcgcgatt ccgcaggtcc gctgcgcagc tttctcgcgg cgacggcgcg
     9721 cgagcacggc atcctgctgg tcggcggcac catcccggtg gctggggagg cgcccggggc
     9781 acgcccgcac gcggcgtgct ttctctacgc cgaggacggc agcgaactcg cccgctacga
     9841 caagatccat ctcttcgacg tcgatctgcc cgatgcacag cgccgctacc gtgaatcgga
     9901 cagttacgca ccgggcgagc ggctcgtgtg cgcgccgacc gcgtgcggca tgctcgggct
     9961 gggtgtgtgt tacgacctgc gctttcccga gatgttccgc gcgctgggcc agcgcgggat
    10021 ggatgtgctc gcgctgccct cggcgttcac gcgactcacc ggcgaagcgc actggcacgt
    10081 gctgctgcgc gcacgcgcga tcgagaacca ggtctacgtg atcgcgcccg accagggcgg
    10141 acggcattcg gccacgcgcg agacctgggg cggctcggtc atcatcgatc cgtgggggcg
    10201 ggtgctggcg agcgcggcca gcggtgaggc ggtgatcgcg gccgaggtcg acgccgcggt
    10261 gctgcgcgac gcgcgcgagc ggctcccggt gcgcgcgcac cagcgttttt acgttcccgg
    10321 ctgatccggc agcagcgcga ggatcacgcg gtagatctcg cgccgtgcgt ggcgggcgcg
    10381 ggcggcatcc cggggggcga gcgcctccgt gcgcaattcg gtgaggcgcg cgctgtcggc
    10441 ggcgccgagc gggaagccga gtgccagcgg accctgttcc agcaccacgt cgcgcagcct
    10501 ctcggcgcga tgatgcagcg cggtctcggc gcgagcctgg gccgacagct ccgtgaccgg
    10561 ccgcgccagc gcctcgatgt cctcgtcctc gagcaggtga gcgatcagcc gcacctggcg
    10621 attgcgcgcg ccgctggcgc tcatggcgcg tgccatctcg agcgcctcgc gcgtgcgttc
    10681 ggcgagcgcc agcctgcgaa agcggctctg cggcagcgcg atcagcgctt cggccagcgt
    10741 ctgcagcgcg cggtgctcgc gcttgcgctg cgatttgctc ggcgggagtt cgtcgtcggc
    10801 ttgcggcttt ctggacatgc gctcggacct gttcaggcgt agaagagggt ggcgaggccg
    10861 aggaaggaca ggaagcccac ggcgtcggtt atcgtggtca gtaccacgct gccggccagc
    10921 gccggatcga tccgcaacga gcgcatcagc atcggcagca gcacgccggc ggcggctgcc
    10981 gccagcaggt tgatcagcat cgccgcggcg atgcacagcc cgatcaggta gtcgccgaac
    11041 cacagcaccg cggcggcggc acacaccagc gcccacagca tgccgttgag gccggccagc
    11101 atgagttccc ggttcagcag ccagccggca ttgctgcgct cgatgtggcc cagcgccatg
    11161 ccgcgcacca ccacggtcag cgtctggctg cccgcgacac cgcccatgct ggcgacgatg
    11221 ggcatcagca ccgccagcgc caccaccttg tcgatggtct gctcgaacag gttgatcacg
    11281 gaggaggcga tgaacgccgt cagcaggttg atccccagcc agatggcgcg ccgcggcgtg
    11341 gccttgagca ccggcgcgaa ggtgtcttcg tcctcgccga ggccggccat gcccatgaac
    11401 gaatgatcgg cgtcctcgac gatcacgtcg accacgtcgt cgatcgtgat gcgcccggcc
    11461 agcttgccct cggaatccac caccgccgcc gacacccagt cgtggcgccg gaacagtttc
    11521 gccacctcgc gcgccggcat gtcgaccgtt atcgggtcca cgtcggtctg catgatctcg
    11581 cgcaccgtga gttccgggtc gcagaccagc agcttggcca ccggcagcag gccgaggaag
    11641 tggtcgttgc ggctgaccac gaacagggcg tcggtcgcat gcgggatctc gccgaggcgg
    11701 cgcaggtagc gcagtaccac atcgacggtg tgccgcgggc gcaccgtgat ggtgtcggtg
    11761 ttcatcaggc cgccggcgct gtcctcctcg aaggagagca cgcgctcgac gcggcggcga
    11821 tcctgcgcgt ccatgccgcg cagcacgtcc tgcatgatgc gccccggcag ctgttgcagg
    11881 atgtcggcca cgtcgtcggg ctcgaggtgc tccatcaggg ccagcacgtc gatggcatcc
    11941 atctgctcga gcagcgcgcc cttgacgtcg tcaccgagct cctgcaggac ttcgccttcc
    12001 tgctcggcat cgatcagctg ccagaggacg tggcgtgcgc ggggcgggga ggattcgagc
    12061 aggtgcgcga cgtctaccgc gctcaggtgg ttcagcagcc gcttggccgg tgccagcgtg
    12121 ccggcgtcga tcgccgccag cagttcctcg agtcgcagcc ggatcgagcc gcgcccggac
    12181 gcaagcgtca ttcgtcttcg ccgaaacggt cgttgatcag ctcggcgacc gcctccaggg
    12241 ctgccaccgc gtcatcaccc tcggtgtgca cggtgaccgc agtacccttc ccggcggcca
    12301 gcatcatcac ggccatgatg ctcttggcgt cggcgccctt gccgtcgcgc tcgatgcgca
    12361 cggtgctgga aaaacgcccg gcggtcgaaa ccagcttcgc ggcggcgcgc gcatgcagcc
    12421 cgaggcggtt gatgatgacg agttccctgc tgaccatgtt cagttctgcc tgttgccggc
    12481 gagatcgcgg tggcgcacct gcacgcccgg gaacaggtcc cggaagtggc gctcgagctg
    12541 ctcgcacatg tagacggagc ggtgctggcc gccggtgcag cccagcgcga ccgtgacgta
    12601 gctgcggctg ctgcgcgcga agcgcggcaa ccagcgagcg aggtatgcgg tgatgtcgcc
    12661 cagcatctcc ccgacctcgg gccgctcctg caggaatttc gccacgtcct cgtccagtcc
    12721 gctcagccgg cgcagtgccg ggttccagtg cgggttgggc aggcagcgca ggtcgaatac
    12781 catgtcggcg tagatgggga tgccgcgctt gaagccgaag gactcgaaca ggatcaccat
    12841 gctgccatcg gtctccacgg ccaccgtccg gccgaccagc tcgcgcaggt cgtgcggaga
    12901 cagcgcgctg gtgtcgatgg tgagcgccgc gcgctcggcg atggctgcga gcagcatgcg
    12961 ttcggccgcg atcgcctcgt tcagcgaggt attcctgttg ctcagcgggt gcttgcgccg
    13021 cgtctcgctg aagcgcctga tcagcgtcgc gtcatcggcg tcgaggtaga tcacctcggt
    13081 ggggaggtcc tgcggcagca tgcgcaggat gtcgccgaag cgcgacaggt ggcctgccat
    13141 gttgcgcgcg tcgatgctca cggccatgcg cttgttcgtg atgccgtcgc tttcccgggc
    13201 ctgctcgatc agttccggga gcagcagcgc cggcaggttg tcgatgcagt agaagcccgc
    13261 gtcctccagc acgtgcaggg cggtgctctt gcccgagccg gagcggccgc tgacgatcac
    13321 gagctttgcg ggggtgggag tgccgtccat cgcggcgacc tcaggcctcg tcgccctgtg
    13381 cccaggccgc cagcatcgtg gcgcgcagcg actcgtcgtc gcggcaggca cgaagccgct
    13441 cgcgcacgcc gggccggctg aacaggcccg cgaggcgggc cagcacgcgc aggtgctcgt
    13501 cggttgcgcc atcgggcacg acgagcgcga acacgatgtc gaccgggttg tcgtcgatgg
    13561 ccgcgaagtc gacgggccgg gcgagggtga cgagggccgc catcggcgcg ctgcagtcgg
    13621 cctcgcggca atgcggaatg gcgatgcctt cgccgatgcc ggtgctgccg aggcgctcgc
    13681 gcgcattgag gctctcgcag agctggtcct cgatttcctt cccgcgtccg cccgcgatca
    13741 ggcctgcgat cgtctcgagc acccgcttct tgctgccaca atccgcgcca cagagcactc
    13801 gctgcggtgt cagcagtgcg tccagcgccg tgatggtcat ggtctggtgc cgggatctca
    13861 gcgcggcgcg aggccctggt ggtgatcctg gctcttgccc ttgtgcttgt tcagctggcg
    13921 gtccagcttg tcggtgagcg catcgatggc cgcgtacatg tcctcggact ccgcggcggc
    13981 gaatagttcg gcgcccgcca tgcggatcgt ggcctccact ttctggcgca gcttctccac
    14041 gttcagcacc acgtcggcgt gcgtgatccg ggattcgtgg cgctccagcc ggcccagctt
    14101 gctggcgacg tagtcgcgca gcgcatcggt gaggtcgacg tggtggccgg tgatgttgat
    14161 ctgcatgggg tttctcctgc tgtgggcccg gtgtggtgtg cgctgtcgtt gcggtgcgcg
    14221 ccgtgctggc ggcgacatcg tcagaccagc tgcttccttt cgttggacgg cgcaatgccc
    14281 aacgcttcgc ggtacttggc gatggtgcgc cgcgccacgt tgatgccttg ctgttcgagt
    14341 atggacgcaa tcttgctgtc gctcagcggc ttgcgcgggt gctcggcgct gaccagcttt
    14401 ttgatgatag cccgtatcgc caccgatgag cattccccgc cggcggcagt gctcacgtgg
    14461 ctggagaaga agtacttcag ctcgaatatc ccgccgggcg tatgcatgta cttctgcgtc
    14521 gttacccggg agatggtgga ttcgtgcatg ctcaccgcgg tcgccacgtc gtgcaggatc
    14581 atcggccgca tggcctcggg gccgtgctcg aggaagccct gctggcgctc gacgatgcac
    14641 cgcgccacct tgagcagcgt ttcgttgcgg ctctgcaggc tcttgatgaa ccacttggct
    14701 tcctgcaggt tgtcgcgcag gaagacgttg tcggcggaat tgtcggcgcg ccggatcatc
    14761 gccgcgtagt aggaattgac gcgcaggcgc ggcgctattt ccgggttgag ctccaccagc
    14821 cagcggccgt tgcgcttgct cacgatcacg tccgggacca cgtattccgc ggcgctcgcg
    14881 ctgatcacgc tgcccggacg cgggttgagc tgctggatgc agtggatcgc cgccttgagc
    14941 tccggttcgc tgatgtgcag gcggcgcatc agcgtggcgt agtccttggc gcccagcagc
    15001 tggatgtgct tgtcgatgat cacgcgcgcg tgccgcacgg cgggttcttc ggggtcgagc
    15061 tggcgcagct ggatcagcag gcactcgcgc aggtcgcggg cgcccacgcc gaccgggtcg
    15121 aaatgctgga tgcgatgcag gaccgcgacg atttcgtcga tctcgatccc gctctcgggg
    15181 ccgaagccct cggcaaggtc ctcgaggctc acgctcagca tgccgtcggc atcgatggcg
    15241 tcgatgatcg acatcgcgat catgcgatcg gtttccgaca tcggcgtcag gttcagctgc
    15301 cacagcaggt ggtcggaaag cgtggaggcc tcggcggtgt actgctcgaa gtcctgcccc
    15361 tcgtcgccgc tgcccgccgg gccggcgctg ccggtctcgt agatatcgtc ccagctggtg
    15421 tccacggcga gatcggaggg catcgattcg gcggtcacgt catcgaccgt gtcgggctct
    15481 gcgacttcgc gctccgccga tccggcgtac tcctccggga gctcctgctg cgagctcgcc
    15541 atccccggca cgacatcgtc ggcaggcgtg ctctcgacca gttcgagcag cgggttgctg
    15601 tcgagtgcct cctggatttc ctgctgcagg tccagcgtcg acagctggag cagccgtatc
    15661 gcctgctgca actggggcgt catggtcagg tgctgaccca gtttgagctg gagcgactgc
    15721 ttcatgagcg gtgtacggaa accctgtcgg tgatggtggc caaccgggaa gagcgggtgg
    15781 caaattcagt gtagtcgggg cggcaaatgc atgcaacagt cgcgccagct tatgcattta
    15841 catgcggaac tgctgcccca gatagatttc tctggccttg tcgttggcca ggatcgtggc
    15901 ggcgtcgccc tcggtgatga tgtggccctc gcccacgatg taggcgcgct cgcagatgtc
    15961 cagggtctcg cgcacattgt ggtctgtgat cagcaccccg atgccgcgcg cctgcaggtg
    16021 gcgcacgatg tccttgatgt ccttcaccga tatcgggtcg acgccggcga agggttcgtc
    16081 gagcaggatg aaggatggat cgctggccag cgcgcgcgcg atctccacgc gccggcgttc
    16141 gccgcccgag agcgcgatgc cgcgtgcgtc gctgaggtgc gtgaggttga attcctgcag
    16201 cagctcttcg agcttctcct gcatgcgctg gcggctgagc gtcttgcgtg tctgcaggat
    16261 cgccaggatg ttctgcgcca ccgtgagacg ccggaacacg gaggcctcct gcggcaggta
    16321 gccgatgccc ttgcgcgcgc gcccgtacat gggcagccgg gtgatgtcct cgccgccgag
    16381 caggatgcgc ccggagtcgg ccggcacgat gccgaggatc atgtagaagc acgtggtctt
    16441 gccggcgccg ttgggtccga gcaggcccac gatctcgccg ctgtccatgc tgatggagac
    16501 gtcgcggatg accttgcggc tccggtagga cttcgcaagg ttcagcgctt caaggcgcgc
    16561 catcggggga tgcctcggct ggcggctgtc cgtcgctcgc cggcggcgcc agcgtggcgc
    16621 gccgcggctg gatcaccatc tgcacccgcg aggcgcccga ttccgcgctg cccgagctgg
    16681 ccttgacgcg ctgctcctgc acgaaatact cgatgcgctc gcccttgacc gtcgagcctt
    16741 cctgggtgac cgcggccttt tcgatcagcg tgaggatctc gcgcgccacg tcgtactgga
    16801 tcgtggtcgc gcgcgcgtac accggctcgc gctcgggcga gggcttctgg tgcatcttcg
    16861 cgggcgcacc gagggcgtcg atgcgggtga cctggtcccc gtccatcgcg atcgtcacgc
    16921 ggtcggccga tatgtgcagg ctgccctggt cgatctcgac gtcgccggta tagacggtga
    16981 tgcccttgcg ttcgtcgcgc tcggcgcgat cggactggat atagatgggc tgattgcgat
    17041 cgtctggcaa tgcgaggccc ggccgcggtg ccagcacgcc ccatagcacg agcgccgcgg
    17101 ccctggccgc gatcgtgcgc aacctgagcc ggccaatacg caatacccct gtctcagcgc
    17161 ccacctgtcc tcctcattgc tcgtgattcg atcatagtcc acggtgtccc gcggtgccgc
    17221 cggcctcggg cagatagggc gcgagcgcct ccgtccactg ctcgcgggcg cgcagcagca
    17281 tctcgcaggc ctcgcgcacg gcgccctcac cgccgcgggc gcggctctgc cacgcggccc
    17341 ggcgcgcgat ctcgctgctg gcattggcca cggtcatgcc gagccccacc gccaggatgg
    17401 cgcgcaggtc aggcaggtcg tcgcccatgt aggcggtctc ggccagtgtc agcccggtgc
    17461 tggccaggag ttcccgtagc gcctcgagct tgtcctcgcg cccctgcacg cagtggtcga
    17521 tgccgagctc gcgcgcccgg cgttccaccg cgcccgaacg gcgcgcggtg atgatcgcca
    17581 cctggatccc gcagcggcgc acgagcttga tgcccagccc gtcgaggatc gagaagctct
    17641 tcatttcctc gccgtcgccg ctgtagtgga tgcggccatc ggtcagcacg ccgtcgacgt
    17701 cgagcgccag caggcggatg cgccgggcga tggctttcag ttcctcggtc atgcgtggct
    17761 ccgggccggg cgcgcgctca ggcaatgccc gcgcgcagca ggtcgtgcag gtgaatgacg
    17821 ccggtggggc gccgctcgtc gtcgaggacg accagcgagg tgatcttgct ctcctcgatc
    17881 aggtgcaatg cctccgcggc cagcgcctcg ccgttgacgg tcttgcagcg cgtcgtcatg
    17941 aagtcgccga tgcgctcgtg ctggaagtcg atgccgcggt cgagcgcacg gcgcaggtcg
    18001 ccgtcggtga agatgccgag caggtgtccc ccggggtcgg ttaccgtggt catgccgaaa
    18061 cccttggcag tcatctccag cagcgcctcg cggatcaccg tttccggggg cacgcgcggg
    18121 acctcgcggc cctggtgcat gatgtcgttg acccgcagca gcagcttgcg tcccagggcg
    18181 ccgcccgggt gcgagaacgc gaaatcctcg gccgagaagc cgcgcgcctc gagcagcgcg
    18241 atcgcgagcg cgtcccccat caccagtgcc acggtggtgc tggaggtcgg cgcgaggtcg
    18301 agcgggcagg cctccgtttc cacgccgacg tcgaggttca cgtcggccag gcgcgccagg
    18361 gtcgattcgg gcttgccggt cagggcgatc agcggcgcgc ccatgcgctt gatcagcggc
    18421 aggatcgtga ggatctcggg cgtggtgccc gaattggaca gcgccagcac cacgtccttg
    18481 ccggtgatca tgccgaggtc gccatggctc gcctcgccgg gatgaacgta gaacgccggc
    18541 gttccggtgc tcgccagcgt tgccgcgatc ttgttgccga tgtggcccga cttgcccatc
    18601 ccggtgacaa cgacccgccc ggcgcagccg agcaggatct cgcaggcacg cacgaaggat
    18661 tcgtcgagcc gctcgcgcag acgcgtcacg gcctcgatct ccagtgccac ggtgcgttgg
    18721 gccgactgca gccagggtga gctcggggag gcgttgtccg cagccatggt cggactcacg
    18781 gggtgttcgt tgtcgcggcg cgacgccggt gccgcaggcc gcccaggggc tggcacggtg
    18841 gtgtcggacg gcgcgtgcaa atggcggtga aagtcacgga cgcgggagtt cactgtggtt
    18901 ctgtcgggcg cgtatgatag ccggtttccg gattcaatgc cgtgctgcgg cggggcggac
    18961 ccagcgggtt tgcggtaccc gtgaccgcac agcacggctc tcgccgcatc gatacggggt
    19021 caagcgcaat gacggaagac gaaatcaggg ctctggtgat ggccggcatg ccgggcgccg
    19081 acgtcgcggt gggcggtgac ggctaccaca tcgatctgac ggtggtcagc gacgcattcg
    19141 agggcctcaa tcgcgtcaag cgccagcagc tcgtctacgg cgtgctggcc gacgtgatcc
    19201 gttccggcgc gctgcacgcg gtgaacatcc gcgcactgac agcggccgag agccaggcga
    19261 cgatctgacg cggggcgctg cattgatgga aaagttttcg atccacggcg gcaggcgcct
    19321 cgacggggag atccgcatct ccggtgccaa gaacgcggcc ctgccgatcc tcgccgcgac
    19381 gctgctgacc gacgatacgc tgcgcatccg caacgtgccc gatctgcagg acatcaccac
    19441 gatgatcgac ctgctggcca gcatgggcgt cgtgctgcgc cgcgacgaac gccacggtct
    19501 cgaggcggac ggcgcgaaca tcggcagttt cacggcgccc tacgagctcg tgaagacgat
    19561 gcgcgcctcg atcctggtgc tgggcccgat gctggcgcgc ttcggccagg cgcaggtctc
    19621 gttccccggc ggctgcgcca tcggcagccg gcccatcgac ctgcacctgc acggcctgca
    19681 ggccatgggt gccgagatcg agatcgacgg cggctacatc aatgcccggg tgcagggcag
    19741 gctgcgcggc gcccacgtgc tgttcgacaa ggtcaccgtg gggggcacgg agaacctgtt
    19801 gatggcggcc tgcctggccg atgggcgcac ggtgctcgag aacgccgcgc gcgagcccga
    19861 gatcgtcgat ctggcccgct gcctgcagcg catgggcgcg cagattgccg gcgccggcac
    19921 ggacacgctg gtgatcgagg gcgtcgagcg cctgcacggc tgcgactata ccgtgatgcc
    19981 ggatcgcatc gagaccggca ccttcctggt tgcggcggcc gcgacgcgcg ggcgcgtgaa
    20041 gctgcgcgac acgcgcgccg acatcctcga ggccgtgctg gtaaagctga ccgaggccgg
    20101 cgcgcacatc gaggagggcg aggactggat cgcgctcgac atgcaggggc ggcgaccgcg
    20161 ggcggtcagt atccgcaccg cgccctatcc ggcgttcccg accgacatgc aggcgcagtt
    20221 cactgcgatg aacgcggtgg cggaaggctc gtccacgatg accgagacca ttttcgagaa
    20281 ccgcctgatc caggtgcacg agatgaaccg catgggggcg cacatcgtcg tcgaggggca
    20341 taccgccatc gtcgagggcg tgtccgcgct gcgcggtgcg ccggtgatgg cgacggacct
    20401 gcgggcctcg gcgagcctgg tgatcgccgg gctcgtggcc gagggtgaga ccgtggtcga
    20461 gcggatctac cacatcgatc gcggctacga atgcatcgag gagaagttgc agatgctggg
    20521 tgccgatatt cgccgggtac gcgcatgagc gcctcagccg gcacgccgct gacgctggcg
    20581 ctgacgcgcg ggcgcatcct caaggactgc ctgccgctgc tggcggccgc cggcatcgtg
    20641 cccgaggaag acccgggttc gagccgcaag ctggtcatag gcaccaaccg cgcggacgtc
    20701 aggttgctgg tactgcgcgg ctcggatgtc tcggtgtacg tgcgccacgg cgcggctgat
    20761 gccggtgtcg tggggcgcga catgctgctc gaggccggca gcgagggtct ctacgaaccg
    20821 ctggatctgg gaatcgcgcg ctgccggctg atgacggccg gcctggtcga gcgagcgccg
    20881 ctgcgctcgc gggtgcgcgt ggccacgaag ttcgtcaacg tggcgcgccg tttctatgcc
    20941 gcccagggcc tgcaggccga catcatcaag ctctacggcg cgatggagct cgccccggtg
    21001 ctggggctcg ccgagcgcat cgtcgatatc gtcgacaccg gcaacacgct gcgcgccaac
    21061 ggcctggagc ccatcgagac catcgccgag atcagttcgc gcctggtggt gaacaaggcc
    21121 gcgatgaaac tgcggcccgg gccgctggga gagctcatcg aggggctcgc agcggcggtc
    21181 gggcagcgcg atgcggctgc gccccggggt ggggcgggcg gcgcgccatg atgcgtcgcc
    21241 tcgacgcgtc ggcacccggt ttcgcgggcg aactggcggc actgctggat ttcagcgcgc
    21301 agcgcgacgc gggtgtcgtg gagcaggtcg ccgcgatcat tgccgacgtg cgcgcgcgcg
    21361 gcgacgcggc gctgctcgaa tacaccaacc gtttcgatgc gcgcgcgctc gttgaggcca
    21421 ccggactgga ggttgcgccc gccgcgctgg cgcaggcgtc ggcgcgcatc cccgcgcagc
    21481 tgggcgaggc gctgcaggca gccgcatcgc gcatcgagtc ctatcacctg cgccagcgcc
    21541 aggaatcgtg gcaataccgc gacgccgacg gcacgttgct cgggcagcgc atcaccgcgc
    21601 tcgatcgcgc cgggctctac gtgccgggcg gcaaggccag ctatccctcg tcggtgctga
    21661 tgaacgccat tccggcgcgg gtcgccggcg tgggcgagct ggtgatggtc gtgccggccc
    21721 cgcgcggaga actgagcgac gcggtgctgg cggccgctca catcgcccgc gtggatcgcg
    21781 tgttcacgat cggcggcgcg caggcggtgg cggcgctggc attcggcacg gccacggtgc
    21841 cgcgcgtcga caagatcgtc ggtcccggca acgtgtatgt cgccgaggcc aagcgccagg
    21901 tgttcggcga ggttggcatc gacatgatcg ccggcccgtc cgaaatcgcg gtgatctgcg
    21961 acggcgccac cgatcccgag tggatcgcgc tcgacctgtt ctcgcaggcc gagcacgacg
    22021 agcacgccca gtccatcctg atcagccccg atgccgcctt tctcgatgcg gtggcgaacc
    22081 gtatcgcgcg gctgctgccg actatggcgc gcgcggacat catcgggcgc tcgctggcgg
    22141 cgcgcggcgc gttcatcctc gtgcgcgatc tcgccgaggc cgcagagatc agcaaccgca
    22201 tcgcgccgga acacctcgag ctctcggtcg ccgagcccga gcagctgctc gcggcgatac
    22261 gccacgccgg cgcggtgttt ctcgggcgcc acacgccgga ggccattggc gactactgcg
    22321 ccggacccag ccacgtgctg ccaacggcgg gcacggcgcg tttcgcctcg ccgctgggcg
    22381 tgcacgattt ccagaagcgc agttcgctga tcggctgttc gccgggcggc gcggaccggc
    22441 tgggccagct cgcgcgcgtg ctcgcggaat ccgagtcgct gcaggcgcac gcgctctcgg
    22501 ccgcggcgcg ggtgcgcaaa ccgggagagg gcgcatgacg cgacgcgatg ccgcggactg
    22561 ggtgaatgcg ctggtgcgcg ccgacgtgcg cgcgaccgcc gcctatcacg tcgccgatgc
    22621 gcgcggcatg gtcaagctcg atgccatgga aaaccccttt gcgctgcctg cgcagctgca
    22681 gtcgctgctc ggcgagcgcg tcgccaccgc ggcgctcaac cgctacccgg atccgcaggc
    22741 gagggcgctg agcgcaagca tccgcagcac cttcgggatc gatccgcgct gggggatcct
    22801 gctcggcaac ggcagcgacg agatcatcca gatgctgatc gccggcgtgt cggggcccgg
    22861 gtgctgcgtg ctcgccccgg caccgtcctt cgtgatgttc cggctgattt cgcagtggct
    22921 gaacgtgcgc ttcgaggaag tgacgctgcg cagcgatttc cagctcgacg tgcccgcgat
    22981 gcgcgaagcg atacggctgc accggccgcg gctgatcttc cttgcctgtc cgaacaaccc
    23041 caccggcaac ctgttcgacg aggcgagcct gcgcgcggtg ctggaggcgg ccgatgcgct
    23101 ggtcgtgatc gatgaggcct atctcgcgtt cgcttcgcgc gaccacaccg ccctgctcga
    23161 cgaatacccg aacctgctga tcatgcgcac gttgtcgaaa ctcgggctcg cggcgctgcg
    23221 cctgggttac ctggtcggcg atccgcgctg gatcgcggag ttcgacaaga tccgcctgcc
    23281 ctacaacatc ggcacgctga accaggtcgc cgccgaggtg gcgctggagc atttcgacgt
    23341 gctggacgcg cagacgcggc tgatcgtctc cgagcgcgcc cggctgctcg tgcggctgca
    23401 ggccgatgcg aggctgcatt gctggcagag cgaggcgaat ttcctgctgg tgcgcccgca
    23461 cgcggcgtcg gcccggaccg tgcatgccgg catggcccgg cgcggcgtgc tggtgaagtg
    23521 tctcgacggg gggcacccgc tgctcgcgaa ctgcctgcgc ctgacgatcg gggcgccgcc
    23581 cgagaacgat gccatgctcg cggcgctgga tgccgagctg gccgttcagt cctgagggcg
    23641 ctgccccagg gttccggcga gccgggtgag cttctcgccg cgatagattt ccagttcgaa
    23701 cgcgtccccg ggagccatca gtgctatgcg gtacatcgca ttgcgtccct ggctggtcgc
    23761 ggtgccgttc agcgcgagca ggatgtcgcc ggcctgtacg ccggcggtct gcgccgggct
    23821 gcccggctcg acttcgctca cgaccagccc cgtgtgcgct ccgtcggcgg taacgatgcg
    23881 cgctggcaac ggttgcactt ccacgcccag ccagccgcgc accacgtagc cgtgcgcgac
    23941 gatgtcgcgc agcaccttga tcgcgtagtc cgccgggatc gccagcccga tgccctgcga
    24001 tccgcccgag cgtgaataga tcgcggtgtt gatcccgagc aggttgccgc gcgcatcgag
    24061 cagcgcgccg ccggagttgc cgggattgat cgcggcatcg gtctggatgt agttttcgta
    24121 ggtgttcagc tgcaggccga agcggccggt cgcgctgatg attccctgcg acacgctctg
    24181 gccgaagccg taggggttgc cgatcgcgag caccacgtcg ccgacctccg cggcgtcggg
    24241 gttgccgacc gggatcggct gcaactgcgg caggtctatg tcgagcaccg cgagatcggt
    24301 gtcgggatcg gtgccgatca ctttcgcgat cgcatcgcgt ccgtccgcga gcagcaccag
    24361 gatctcgttc gcaccttcga tgacgtggtt gttggtgagc aggtaaccgt cgccggacat
    24421 gatcaccccg gagcccaggc tgcgctggat gcgctcgcgc tgcggagccg tgccgcgatt
    24481 gagcagccgc tggaagaagg gctcgtcgaa cagcgggctg taaggacggc gcacgacctt
    24541 gcgcgtgtag atgttgacca cggcgggcgt ggcacggcgc acggcagcgg cgtaggacgt
    24601 cggtgcatcg acgctggcag cctcggtgct cgtcgtgggc cgcccggcga gttcacggta
    24661 atgcagggtc tgcaggatca gcagcgccgc catcagtccg gtggcgaccg gccagcccat
    24721 ggcgcgccag ccgttcaacc ccggcgtttc ggccaagagc cgtccctgcg tgcgggtgtg
    24781 cgctcagccg gcatggaccc tttccaggtc atattcctcg ttgagcatgc cgggctgctg
    24841 cgtcgaaccg cgcggcgcgt agtcgagtgg cgggttcacg gaaggcggca ctgaagcggc
    24901 cgagcgatat cccgggccgc cgaggctggt cagcaacggc ggctcgtcgc cgggctggcg
    24961 cggttcgcac agccccagcg cgccttcggc aaggtgctgg tgcaactcgc ggtaatcttc
    25021 cgtgagccgg ttcactcgtt cggcggtgcc cttgaagtgc gcggtgacct gcgacttgta
    25081 gtcgcgcaac gccagttcgt tctcgagcag ccgccgctcg aggtcgcgct gacgctgttc
    25141 cgcggggtcg aattggcgcc ggatcagcca cccggctgcg acgccggcga gcaggcatgc
    25201 gacgacggtg atccagaaaa cggcagaact gtacaacgga acggctctcc tgagtcggtt
    25261 tggcgtactc cgccagagta taaccactat gccggggtcg aggtaggcgc tcaacggttg
    25321 cgaccgcggg ggcgtctcta tacattgcgc aaccgcgcag ggagttgcat cgtggatgag
    25381 ctgaggggaa tgggaccggc agagcgctat cgcgcggaat tggggcgacc cggtttcgtg
    25441 cgcgacccgg cgcaggagcg ggcggtcggg gagttgcaac gcgtctacga ggaactcacg
    25501 gcgcgacccg cgcgcggtcg catgcgccga ctggtgtcgc gggtcgcggg cgacagccgt
    25561 gagccggtca tggggctcta tctctggggc ggcgtggggc gcggcaagac gcacctgatg
    25621 gatctctttt acgcgagcat cccgttcgac gagaagctgc gcacgcactt ccatcgtttc
    25681 atgcagcgcg tgcaccgcga actgcgcgac tgcgccgggg agaagaaccc gctcaggttg
    25741 atcggaaaga ggatcgcacg cgaggcccgg ttgctctgct tcgacgagtt cttcgtcgcg
    25801 gacatcaccg atgccatgct gctcgcaggt ttgttcgagg aactgttcgc gcgcggcgtg
    25861 acgctggtgg cgacatccaa cgtggagccc gacgatctct accgcgacgg cttgcagcgc
    25921 gcgcgcttcc tgccggccat cgcgatgatc aagcgccata cgcgggtcat caacgtcgac
    25981 ggcggcatcg accaccggct gcgccggctc gagcaggcgc agctctacca ctgccccctg
    26041 ggcgacaggg ccgacgaaag cctgatgcga agctttacca gcctggcgcc ggaggagccc
    26101 gtgcgcgagc gtgtgctcga ggtggagggg cggcccatac gcacgcgctt ctgcgccgat
    26161 gacgtggtat ggttcgactt cgccgagctg tgtgacggcc cgcgcagcca gaacgactac
    26221 atcgagctgg cgcgcgagtt ccatgcggtg ctggtgggtg gcgtgccggt gttcaccgcc
    26281 gacaaggacg accaggcgcg gcgattcatc aacctggtcg atgaattcta cgaccgcaac
    26341 gtcaagctgg tgctgtcggc ggccgcaccg ctggccgaac tctatgccgg tggacggctc
    26401 ggtttcgagt tccagcgcac cgtcagccgc ctgatggaga tgcagtcgca tgagtacctg
    26461 gcgcgcgcgc atcgcgcgtg agcgcgggcg gcgtgaaaat tcccggcatc gtgcttgtcc
    26521 gggtcaatat ccgtggtgta gtattcgcac ccttttccgg gccctttccc gttgaggcga
    26581 gcagatgaag acaatttccg ccaaacccgg cagcgttgag cagtcctggt acgtaatcga
    26641 tgccaacggc cagacacttg gccgcatggc cagtgccatt gcgtcgcgcc tgcgcggcaa
    26701 gcacaagccc gagttcaccc cgcacacgga caccggcgac ttcatcgtcg tggtgaatgc
    26761 cgagaaggtg cacgtgaccg gcaacaaggc gaaagccaag atctatcacc accacacggg
    26821 ctatccgggt ggtctgaagt cgatcagctt cgagaagctg atcgagaaag cgcccgagca
    26881 ggcaatcgaa atggcggtca agggcatgct cccgaagaac ccgctgggcc gggccatggc
    26941 gcgcaagctg aaggtgtacg ctggcagcga acacccgcac gcggcgcagc aaccgaaagc
    27001 actcgagctc tgactggaac atacaaacac atggccgcta cgcagtatta cggaaccgga
    27061 cgacgcaaga ccgccactgc ccgagtcttt ctgagacagg gtcggggcgc catctcgatc
    27121 aatgatcgcc cgctggatca gtatttcggg cgccaggtgg cgcgcatgat cgtgcgccag
    27181 ccactggaac tggtcgggct gggcgagaaa ttcgacattc acgtctcggt tgccggcggt
    27241 ggcagcttcg gccaggcggg cgcgatccgt catggcatca cccgcgcctt gatcgagtac
    27301 gacgagacgc tcaaatccgc gctgcgccag cagggttatg tgacccgcga tgcgcgcgag
    27361 gtcgagcgca agaaggtcgg cctgcgcaag gcgcgcaagc gtccgcagta ctcgaagcgc
    27421 tgatcgtggc gcccgggttg ccgcccgggc cccgatcaat gctcatcctc cggaacgccc
    27481 ggtccagcaa tggcccgggc gttttgccgt gcggggcggc agtttgcgcg caacgatgcc
    27541 gatgacagcg caatatcccc aaattccttg tcaccggcag ggaatttctt tagcattccg
    27601 gccgcaaaat tcgccccgcg gcgcggtgtt gcgtccatcc aggattggag aatctccctc
    27661 gtgagtaaaa aaggcgttaa caaggggagg cggcagttcc tgaccgccgc cacctcggtc
    27721 gtaggtgcgg ctggagtcgt cggcgtagcc gtcccgttcc tgtcttcctg gaatcccagc
    27781 gccaaggcca aggcggcagg ggcgccggtg aaggcggaca tcagcaagct cgagcccggc
    27841 cagatgatcg tggtcgagtg gcgcgggaag ccggtctacg tggtgcgccg cacccaggag
    27901 agcctggatg cgttgacgac cggatcgctg gtcgaagtgc tgcgtgacgc ggattctgcc
    27961 gaatccgcgc agccggctta cgctggcggt gtccagcgct cgatcaagcc ggaattcctg
    28021 gtgctggtcg gactgtgcac ccacctgggg tgcgcgccga agtaccggcc cgacgtcggc
    28081 gcgaccgatc tcgggggcga tcagtggctc ggcggcttct tctgtccctg ccacggttcg
    28141 aagttcgacc tcgcgggacg cgtgttcaag ggcgtgccgg cgccgctgaa tctcgaggtg
    28201 ccgccccata tgtacgaggg cgacaaggtt ctggtaatcg gcgttgatca ggagaccgcg
    28261 taatgaactg gcttgttggt ctgcgggact gggtcgatgc gcgtctgccg atcgtgcgcg
    28321 cgtgggacac ccacatgggc aagtactacg cgccgaagaa cttcaacttc tggtacttct
    28381 tcggcgtgct ctcgctgctg gtgctggtca accagctgct gacaggcgtc tggctgacga
    28441 tgagcttcga gccctcggcc gagcgcgcgt tcgccgccgt cgaatacatc atgcgcgatg
    28501 tcgagtacgg ctggctgatc cgctacatgc actcgaccgg ggcctcggcg ttctttgtcg
    28561 tggtgtacct gcacatgttc cgcgggctgc tctacggctc ctatcgcaag ccgcgcgagc
    28621 tggtctggat cttcggcatg ttcatctact tcatgctgat ggcggagggt ttcctcggct
    28681 acgtgctgcc ctggggtcag atgtcgtact ggggtgcaca ggtgatcatc tcgctgttcg
    28741 gcgcgatccc gtacttcggc gaagacctgg tgcaatggat ccgcggtgac tacctgatct
    28801 cgggcatcac gctgaaccgt ttcttcgcgc tgcacgtggt cgcggtgccg atcatcctgc
    28861 tcgcgctggt ggtgctgcac ctgctggcgc tgcacgaggt cggctcgaac aaccccgacg
    28921 gcgtcgagat caagaagaag aaggacgcca acggcgtgcc gc
//
LOCUS       DAORUQ010000022        28198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1230_length_28198_cov_6.756991, whole genome shotgun sequence.
ACCESSION   DAORUQ010000022 DAORUQ010000000
VERSION     DAORUQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 28198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28198
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_28198_cov_6.756991"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..986)
                     /gene="dmeF"
                     /locus_tag="PLW13_03600"
     CDS             complement(54..986)
                     /gene="dmeF"
                     /locus_tag="PLW13_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HQY69485.1"
                     /translation="MHDHDMNGWRHSHDFGGHTDRAETSTRRVIALTAVMMVIEIVAG
                     TASGSMALLADGWHMATHVAAFGITLFAYRYARIHATNPRYSYGTGKVGVLGGFASAV
                     ALAMVALMMALESGMRLIEPQAIHYSQAIGVAVAGLVVNLVCGAMLHDHHHDHDRAED
                     HDHDHGHHDHNLRAAYFHVLADALTSLFAIVALLAGKYVGWTWLDPVVGIIGALVIAR
                     WAWGLLRDSGHILLDAAADEGTQAAITRVIESDADNRIADLHVWPLGPRHYAASLSVV
                     THDPRPPEHYKALLREIPLLAHVVVEVNRCCEEL"
     gene            complement(1106..1348)
                     /locus_tag="PLW13_03605"
     CDS             complement(1106..1348)
                     /locus_tag="PLW13_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HQY69486.1"
                     /translation="MDSESLRKLVTLPMPFGKYQGRLIADLPGNYLAWFARQGFPPGE
                     IGRLLALMHEIDHNGLKELLAPLRNSPRQGQRATPR"
     gene            complement(1351..2454)
                     /locus_tag="PLW13_03610"
     CDS             complement(1351..2454)
                     /locus_tag="PLW13_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQY69487.1"
                     /translation="MSLKRSNWPEGQLVTLEHVSTVLRDNPLGDPHVRKLHAWLPPQY
                     HASGPRRRFPVLFDLAGFMGSGPAHLAWRGFEENVPERLARLVHQKRMKPAIVVFPDC
                     FTALGGNQYINSSAIGRYADYLTRELIPAIDQEFRTRASRDSRGCFGKSSGGYGAIVH
                     AMKYAGHWGAVANHSGDAYFDFLYRADWPNTLDELAKYRRPARTAGRYDTEKASAALK
                     PGLDDGRVRRFLEHIWKTDRPGHAETMCLMNLAMAASYDPDPRAPNGFRLPFHLETGE
                     LIEARWQRWLAHDPIHMVAAHRANLASMRGIFIDCGWRDQFHIHYGCRQLSRELARHG
                     IVHTYEEFDGTHSGIDYRLERSLPFLTRALAPG"
     gene            complement(2488..3093)
                     /locus_tag="PLW13_03615"
     CDS             complement(2488..3093)
                     /locus_tag="PLW13_03615"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQY69488.1"
                     /translation="MRSSIAARLDTFTPHILSPEELRRAAVVIAIVAGAHGEAACLLT
                     RRPETIKRHAGQFALPGGRVDPGESEADAALRELHEEIGISAARSDILGTLDDYATQS
                     GFRMRPFVLWIEDEAGLAPDPREVARIYRIPLAELASPALPTLHPVPHSEQPAISLRL
                     PTIGDELHAPTGAILYQFREVAFAGRHTRVGHFAQPRFSWQ"
     gene            complement(3120..3317)
                     /locus_tag="PLW13_03620"
     CDS             complement(3120..3317)
                     /locus_tag="PLW13_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY69489.1"
                     /translation="MYVCICNAVTDSEIADAVYNGAATMAHLEDTLLVGTCCGKCRDK
                     ASECLERYSPARPRLSLAMSA"
     gene            complement(3432..3896)
                     /gene="bfr"
                     /locus_tag="PLW13_03625"
     CDS             complement(3432..3896)
                     /gene="bfr"
                     /locus_tag="PLW13_03625"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQY69490.1"
                     /translation="MKGDKQVIQFLNKVLRQELTAINQYFLHARMYKNWGLKQLDEHE
                     YHESIDEMKHADRLIERILFLEGLPNLQDLGTLRIGENTREILEADLALELEAVPVLR
                     EAIAHSESVRDYVSRELFEDILESEEEHIDWIETQLELIGKVGIENYLQSKM"
     gene            complement(3972..4391)
                     /locus_tag="PLW13_03630"
     CDS             complement(3972..4391)
                     /locus_tag="PLW13_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HQY69491.1"
                     /translation="MDVRKLSDAVSVAPQLSVREIADAARLGFRTLINNRPDGEDFLQ
                     PSARELEAAAREQGLEYHHLPVVSGALTERDVHAFRALLAHAEQPVLAFCRSGTRSTT
                     LWALAQAGGGNADAVIAAAAQAGYDVSGLRGILERGL"
     gene            4637..5893
                     /locus_tag="PLW13_03635"
     CDS             4637..5893
                     /locus_tag="PLW13_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQY69492.1"
                     /translation="MSSVRVLLRASLTLAIAGGSAAHATNGYLSHGYGIKAKGAAGVG
                     AALPQDTLTIATNPAGLIEAGHRSDLGVELFVPDRGGEIKGNAFGPDQSFDGNGTEYF
                     LIPELGHSHLVCDDLAVGFAMFGNGGMNTDYDENPYARFGSKGKSYMNFEQLFIAPAL
                     AWEFSPGQTIGLALNLAYQRFEAKGLSAFAPLSADPANLEHNGTDDGFGAGVRIGWLG
                     QITDRLTLGASWQSTTWMQEFDKYAGLFAEQGGFDVPENYVLGAAFKLSDATTVALDW
                     QTIKYNDVASVGNALGQLFLGNPLGSDNGPGFGWQDMSTLKIAVLHQYSDRVTLRAGY
                     SQGDQPVPRDQTFFNIIAPGVVEEHVSLGATFAFNERNELSIAYTRGLEHEVHGKGSI
                     PAMLGGGEANISLAEDSFGLAWSLIY"
     gene            5924..6334
                     /locus_tag="PLW13_03640"
     CDS             5924..6334
                     /locus_tag="PLW13_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HQY69493.1"
                     /translation="MTDLVHLVAGGALIGAGSAVLALANGRIAGISGAFGNLLDGDAG
                     PQRWRLGFLLGLLLPALYLLLNGVDVAMAGGVAWLLVAGLLVGIGTRVGSGCTSGHGV
                     CGTANLSKRSIVATLLFMSAAMATVYVVRHVVQG"
     gene            6412..6780
                     /locus_tag="PLW13_03645"
     CDS             6412..6780
                     /locus_tag="PLW13_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HQY69494.1"
                     /translation="MTDPRNVLAFLDIAGNWNPGLAVVMATAIAITAPALWLLKKRGK
                     TLAGESCDIANRNPIDTSLVVGAVVFGIGWGMSGICPGPGLIIAVGGVPGALLFVAAM
                     AAGMRLSGIVLRRLGSSAAP"
     gene            complement(6823..7665)
                     /locus_tag="PLW13_03650"
     CDS             complement(6823..7665)
                     /locus_tag="PLW13_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69495.1"
                     /translation="MARKRSDTKTVFITGAGGGLGGATARYLAERDWTVFAADFDARA
                     LKALAAETDVIPVQLDVTDATSCEAARRRVARTCDGLDGIVNFAGILAVGSVIEIDPD
                     TIRRVLDVNVMGTVRVNHALFPLLLARKGRIVNISSETGWQSAMPFNGAYALSKHAIE
                     AYSDALRRELMFLDVPVIKIQPGPFRTGMVASIEQNFERVAQASRYFKELLQTLKKAT
                     VREQGTAHDPALLAEVVHTALTTRYPRIAYSVKPDPQRVALSALPDRVADPLLKLALG
                     ALAR"
     gene            7823..8305
                     /gene="nrdR"
                     /locus_tag="PLW13_03655"
     CDS             7823..8305
                     /gene="nrdR"
                     /locus_tag="PLW13_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQY69496.1"
                     /translation="MRCPFCSAADTKVIDSRLVAEGDQVRRRRECLSCAERFTTYETA
                     ELLLPRIIKQSGVREPFNEDKLRQGILRAIEKRPVSMERVELMLSQIKHALQATGERE
                     VASRVVGEQVMRQLKAVDHVAFVRFASVYRSFQDLNEFRAEIERLAEEQDSPPPGAAG
                     "
     gene            8315..9424
                     /gene="ribD"
                     /locus_tag="PLW13_03660"
     CDS             8315..9424
                     /gene="ribD"
                     /locus_tag="PLW13_03660"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQY69497.1"
                     /translation="MSQQITDVRHMAQAIGLARRGLCTTAPNPRVGCVIAQGERVIAS
                     GFHRRAGQAHAEVEALRAAREPVRGATAYVTLEPCSHHGRTPPCADALIDVGVARVVS
                     ASLDPNPLVAGSGLDRLRAAGIAVESGILEREAMALNPGFFKRMRTGMPWVRVKLAMS
                     LDGRTAMADGSSKWITADAARADVQRLRARSCAIVTGVGTVLHDDPRLDVRIATDDGV
                     TPGRQPLRVVVDSQLRMPPSARVIGAAGDTLVATAGRDAAREQALADAGVTLLHLPAP
                     DGRVDLRALLAHLAVAQCNEVLIEAGATLAGAALRAGLVDELIIYMAPTLMGSAARPL
                     LDLPFASMDEKLGLQISDITAVGSDWRITASVGGA"
     gene            9597..10265
                     /locus_tag="PLW13_03665"
     CDS             9597..10265
                     /locus_tag="PLW13_03665"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQY69498.1"
                     /translation="MFTGIIQATGAVRHLERRGGDVRLHVGTGKLELGDVRAGDSIAT
                     SGVCLTVVEFGTDWFAADVSLETLGLTTLGGLAAGARVNLEKAMAVGERLGGHIVSGH
                     VDGVGEVLSMTPDGRSTRYSLRAPAALARYIAHKGSICVDGVSLTVNSVDGAGFELNI
                     IPVTAHETIFGDYRPGTRVNLEVDVIARYLERLLQGDAAAHSGSAHRGVTRELLAQNG
                     FPGT"
     gene            10262..11383
                     /gene="ribB"
                     /locus_tag="PLW13_03670"
     CDS             10262..11383
                     /gene="ribB"
                     /locus_tag="PLW13_03670"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQY69499.1"
                     /translation="MKFDTTQQLIDSFRHGDMVVLLDDDDENLGGVLLAPAQDITAET
                     VNFMARQARGLICLSLTGERCAQLRLPLMVESASHSVYASRFTVSIEAAEGISTGISA
                     ADRAHTIRTAVARGAQPRDLVQPGHVFPLRAEPGGVLKRAGHTEGGCDLARLAGFEPA
                     AVLADVLNDDGTLATGQRLLAFARQHGLRIGTIAGLIQFRLINETTVHRFREGEVQTA
                     FGAFRLHAFRDADDGRLHVALVRGAIDPDAPTLVRVQQAATLRDVLGTEVPGRKPNWN
                     VSRCLECIDREGRGVIVLLSQPETEQQLLASVDAALGGEAATEPTAPEGGQSVLNLVG
                     VGSQILRQLGVGQMRVMGPPIRYNAISGFGLEVAEYVMY"
     gene            11467..11937
                     /gene="ribE"
                     /locus_tag="PLW13_03675"
     CDS             11467..11937
                     /gene="ribE"
                     /locus_tag="PLW13_03675"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQY69500.1"
                     /translation="MAEIRTIEGVFAAGEARFALVASRFNSFVVESLIAGAVDTLRRH
                     GVADAQITLVRSPGAFELPLVVQKVAASGNFDAIVALGAVIRGGTPHFEYVAGECTKG
                     IAQVSLASGLPIAFGVLTVDTIEQAIERAGTKAGNKGAEAALSALEMVSLLGRL"
     gene            11952..12416
                     /gene="nusB"
                     /locus_tag="PLW13_03680"
     CDS             11952..12416
                     /gene="nusB"
                     /locus_tag="PLW13_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQY69501.1"
                     /translation="MSDNALENERRQLAAARRKARHYAVQALYQWAMTGNYLADIEQH
                     FTEEFDFRGADTEYFRELLHGVPADLSAIEAAMEPYLDIALEKLGPVERAVLRLAVWE
                     LKSRPDVPYRVVLSEAVALAKKFGAAESHRFVNGVLDKVARDLRPAEAGRAR"
     gene            12433..13386
                     /gene="thiL"
                     /locus_tag="PLW13_03685"
     CDS             12433..13386
                     /gene="thiL"
                     /locus_tag="PLW13_03685"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQY69502.1"
                     /translation="MALSEFELIARYFDSQDFALPEAEGVLLGIGDDAAILRLPPGED
                     LAVSIDTLVAGVHFPAASSPEQIGHRALAVNLSDLAAMGARPLWFTLALTLPEADETW
                     LAGFSRGLAALARRHHVALVGGDTTRGPLSITIQVHGCVPPGLALRRDGARAGDDVWV
                     SGHPGTAALGLRQLLAGGARDEAARVFAAPAPRVALGLALRGLASAAIDVSDGLLADA
                     GHIAARSGVAIALERERLPFSPQLESLADRDDACALALGGGDDYELCFTAPPRHGAAI
                     AGAAADLGLACTRIGRVLAGQGATCAGFVPRTGGYQHFRGA"
     gene            13424..14062
                     /locus_tag="PLW13_03690"
     CDS             13424..14062
                     /locus_tag="PLW13_03690"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HQY69503.1"
                     /translation="MLRRWMLAATLLPLAVAAESLHVTAAALFEGRAMLGIDGSRRLL
                     RVGETSPEGVKLVSATAREAVIEIDGRRQTLAPGRDTGGGFAAPERKRVAIQRGARHE
                     YLVAGTVNGLQVQFLVDTGANVVAMNGADARRLGVDYRLHGRPGAVQTASGVVQAWSV
                     TLDRVEVAGIAVRNVAASVIEGNYPAQLLLGMSWLGRVTMREEHGVLYLEEK"
     gene            14162..14764
                     /gene="ribA"
                     /locus_tag="PLW13_03695"
     CDS             14162..14764
                     /gene="ribA"
                     /locus_tag="PLW13_03695"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HQY69504.1"
                     /translation="MSVRYIASSRLPTPWGVFDMHGFEDIESEKEHVVLTMGDVSNGE
                     PVLARIHSECLTGDALFSMRCDCGAQLRTALERIAREGRGALLYLRQEGRGIGLLNKI
                     KAYRLQDAGADTVEANEQLGFGADMRDYSICRTMLKHLHITSLRLMTNNPRKVAAIED
                     MGIKVVERISIRTGQNPHNVRYLETKAGKLGHWMDEDPGG"
     gene            complement(14825..16750)
                     /gene="dxs"
                     /locus_tag="PLW13_03700"
     CDS             complement(14825..16750)
                     /gene="dxs"
                     /locus_tag="PLW13_03700"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQY69505.1"
                     /translation="MKLFQEIPVTRPATPLLDRVTFPADLRQLDEAQLLQLANELREF
                     LLYTVGQTGGHFGAGLGVVELSIALHYLFDTPRDRLVWDVGHQSYPHKILTGRREAML
                     TIRKAGGLAAFNNRAESEYDCFGVGHSSTSISAALGMALAARAHGEDRRAVAIIGDGA
                     MTAGMAFEALNHASHTGADLLVILNDNQMSISRNEGGLASYFARIWASRSYNHMREAG
                     KRVLSRLPGPALEIARRTEEHMKGMVAPGTLFEELGFNYIGPVDGHDLPTLLATLRNV
                     RELKGAQLLHVMTVKGKGFAPAERDPVGYHAINKIDPAPRVAGTKPVPVRPKYQEVFG
                     DWLCDMAWRDERLVGITPAMCEGSGMVEFARRFPARFHDVAIAEQHAVTLAAGLACEN
                     MKPVVAIYSTFLQRGYDQLVHDVALQNLDVLFAIDRAGLVGEDGPTHAGSFDLGYLRC
                     VPNMVVMAPSDEDETRKLLSTGFLHHGPAAVRYPRGTGPGATIDPTLEAVAIGKGVLR
                     RAGREVAILGFGSMVAPALAAASALEASVADMRFVKPLDEELILELCASHSLLVTVEE
                     NAVAGGAGGAVAELLAARGIVMPLLVLGLPDRFLEHGKPQQMLHDVGLDAEGIEAAIR
                     ERLAAMRPSRWSAVSQL"
     gene            complement(16841..17749)
                     /locus_tag="PLW13_03705"
     CDS             complement(16841..17749)
                     /locus_tag="PLW13_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017736629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQY69506.1"
                     /translation="MSEVPVNGAHLETYRARAEAALATLFDGELVGRADARLREAMRY
                     SLLAGGKRLRPVLLYAAATAVGGSRASAPDTDRAACAIECIHTYSLIHDDLPAMDNDD
                     LRRGQPTCHRAFDEATAILAGDALQTLGFELLCAADSVDAATRLALVAELASASGSRG
                     MVGGQAVDVAHVGRQITAATLESMHALKTGALIRAALRMGALLGAADVRARACLDEYA
                     RCIGLAFQVQDDILDACGDVAALGKNPGSDAAQTKPTYVTELGEAGARALALELRDAA
                     LATLAPLGGPAAALAEMARFIVSRDR"
     gene            complement(17742..18002)
                     /locus_tag="PLW13_03710"
     CDS             complement(17742..18002)
                     /locus_tag="PLW13_03710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQY69507.1"
                     /translation="MSAPRKPLNFEKSLADLEALVERMEDGELSLEESLKAFEAGIRL
                     TQQCQQALTQAQQRVQLLSVQDGEVHATPFAEADDADEDAGV"
     gene            18238..18993
                     /gene="pomA"
                     /locus_tag="PLW13_03715"
     CDS             18238..18993
                     /gene="pomA"
                     /locus_tag="PLW13_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein PomA"
                     /protein_id="HQY69508.1"
                     /translation="MDLATIIGLLGALLIIMAAIFLGGDPVMFLDAPSFLIVIGGTVF
                     VVMSKFGMNQFAGAFRVAGKAFLFRPEESGELIARIVELANLARRGGLLALEEQPVSN
                     RFLADGLRLLIDGHDAEHVQAALSKDRRQAMERHEWGQRIFLAMADVGPAMGMIGTLV
                     GLVQMLANMDDPKSIGPAMAVALLTTLYGSLLANVVCLPIADKLKLRGAEEATQHALV
                     VDGLLAMQAGQNPRVIQGLLERYLPAGQRPAPA"
     gene            19006..19971
                     /locus_tag="PLW13_03720"
     CDS             19006..19971
                     /locus_tag="PLW13_03720"
                     /inference="COORDINATES: protein motif:HMM:NF006508.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotB family protein"
                     /protein_id="HQY69509.1"
                     /translation="MDEDEQRGSTEIAIPKWLPTFADMMTLLMCFFVLLLTFAEMDIK
                     RYEQIAGSMSEAFGVQRRIVAESVPMGTSVIALEFSPGVPRPTPISEPYRQDDALPEL
                     APDAAADEATPDLELTEQMKRNVEAQIRATQADAAALAEKLTGEIARGEVEIETSGRR
                     IVVRIRERGSFASGSAELKPEYQALMRQFRDVLAAQEGAVEVQGHTDDVPIATARFRS
                     NWELSSARAVSVAEPLLEGGVLDPRRLSVTGFADARPLVANDSADARARNRRVEIVID
                     QGIDRQLREQLDALKTVDPAYYRSLRLDEAGEGRGGAVHGSGGGR"
     gene            19968..20564
                     /locus_tag="PLW13_03725"
     CDS             19968..20564
                     /locus_tag="PLW13_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY69510.1"
                     /translation="MIEKRSGADRRRFFRIDDRLRLAVRRVPGVDAVAGKGHDLRGDE
                     FLAGIDRGIAAIIAAARVQAPAVGELAQLLNRKLDYVIERLGQIETPRAGLPLVEHEV
                     SISACGIGLQSAERYEAGETLQVELMLPPEDTRLCVPARVVRCRDATPGNHLLQLDFA
                     GMDHDDQELLIRFILRRQGQFLQGLREQRDARRARVSA"
     gene            20629..21513
                     /locus_tag="PLW13_03730"
     CDS             20629..21513
                     /locus_tag="PLW13_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY69511.1"
                     /translation="MRYGICLPYMKRDYDRDTILAWCRAIDAGPWDSLSCGERITGYT
                     CDMRVLLSAAAAVTERVRIVPSLYVLPMHSAVWAAKEIATLDVLSGGRVTVTVGVGGR
                     EKDYRAVGASFAHRHERMDQQVAEMRRIWAGEPPFEGADPVGPAPVQAGGPPILIGAM
                     GPKGMRRGAAWAQGVYLFSMNGDKGEMAKMIDMADSAWEEAGRADRPYRMAGFWHSLA
                     DDAEPRLRQYVYDYLKIAGDDVAKLVAGTMTRSNEDAVRRAIDDYRELEVDEIMFSAV
                     TADLAELDRLANLIARIG"
     regulatory      21615..21721
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21781..23934
                     /locus_tag="PLW13_03735"
     CDS             21781..23934
                     /locus_tag="PLW13_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY69512.1"
                     /translation="MRHVSFSSAGSARRRALSLCLAVLGAPHAGAAAAPERLEEIVVT
                     ARLLPKPVDTVPASISVLPAALIRERQANHFEELLNAAPNVNYSSGASRGRFVQIRGI
                     GERSQFVDPVDPSVGLYIDGIDFSGLGNAGTLFDVEQVEVLRGPQGTAFGASAMAGLV
                     NIRSAAPADRTMARVEGGLAEYGGSTLGAVASGPLTADLGARLAVHRNRSDGYIENDF
                     LGRDDTNAIDETTARARLDWQAGEALALGLTLMRVEADNGYDAWSLDNTRHTFSDEPG
                     RDRQTSDAAALRADWTTGAGFDVQAVATWMGSDLDYGYDEDWSYAGICDGTDCEGWEY
                     SSTDRYLRDVEAGSVDLRLVSHPGELTWVAGLYAVNRDTQLERRFHDWDVDAPGLFRS
                     AYDSGHVAAYGELDWAASERVAVSGGLRLQRFDADYDDSLGLREQPGDDLWGGQLSAQ
                     YELAPRSMAYALVARGYKAGGVNGEAIGKAQKSGLDSSIIAFLGTRSEYAAETLWNYE
                     LGWKFASNDEALRVRAALFYMDRDDVQLKAWYNEGPQFVGYTDNAASGSNYGLELEAD
                     WQVFERLRLDASLGLLDTEIGNFVANDPELGFVDKDGREQAQAPNWQFRAGAELALFA
                     RGFARVEYEGKDGYYLSDSDDERSEAYRLLHLAAGYRGDRVEATAWLRNALDEDYVVH
                     GFYFGNDPRTFYVNEAWYQYGAPRVAGVTVSWSFE"
     gene            23945..24199
                     /locus_tag="PLW13_03740"
     CDS             23945..24199
                     /locus_tag="PLW13_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HQY69513.1"
                     /translation="MQISVDLSLYPLRDDYIPVIIDFIHRVQAHEGVEVVRNQMTTQL
                     FGDYGCVMAVLTEELRHSFETHGKAVIVARIVNGDVRANP"
     gene            24209..24832
                     /gene="pnuC"
                     /locus_tag="PLW13_03745"
     CDS             24209..24832
                     /gene="pnuC"
                     /locus_tag="PLW13_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HQY69514.1"
                     /translation="MTAMLAGWLQLHGWEALAVLLGITYLLLAMRESLWCWPAAFLST
                     LIYLFLFWHAELPMESGLQFYYLAMAVYGWWHWRGGTTEAAEPLPVRTWPWRYHVVAI
                     VLVLAATLASGAALTHAGLGRLPYLDSFTTWGAILTTWMVAQKVLENWLYWLVVDGVS
                     LYLYLDRGLHLTALLFVVYLVIVVFGYFQWRRHYREQHAASDAPRAG"
     gene            24813..25685
                     /locus_tag="PLW13_03750"
     CDS             24813..25685
                     /locus_tag="PLW13_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HQY69515.1"
                     /translation="MPRALAELFRDWRPDSLDTTPELEAVFSSGHSNRSFLVRLGRRR
                     IVVRLPEGNGERLGIDRHVEQRVLALADRIGLGAPVLYCDPARGILVTAYLESKPLRV
                     EGIAADHTIDRLAAALHLLHRQELDVPVLNIADRVRAYARELQADDPLAWPRARRWLA
                     SARQVLEQYRFGRWHTALCHNDLLAQNVLEVDGAVRFIDWEYAARGDAFFDLATIAED
                     CGFGALDRSRLLLAYGEIGDTAIERLYRARVLYRLLGVLWHLVRYRGASARTVPALVR
                     HEQALDALLRQGPA"
     gene            25686..26690
                     /locus_tag="PLW13_03755"
     CDS             25686..26690
                     /locus_tag="PLW13_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQY69516.1"
                     /translation="MGARDTAARLLLPALALLLGLAFLLALNTGSSGVLLGPALRDWY
                     AGEDSAALIILREIRLPRALAALAVGATLGVAGAALQGMLRNPLAEPGLVGASNCASL
                     GAVLVLYFGLAPMHSLLLPAAGILGAVAALALLRLLVGDYARVATLILAGVALNAFAG
                     AAIALALNLAPSPYAMHEIVYWLMGSVANRSFEELAFSLPFMGLALALLLSTGRYLEA
                     LSLGEDTAHSLGFGARSLPWRVMGGVAIGVGAAVAISGTISFVGLVIPHLLRPFVGHR
                     PGRLLVPSALGGALLVLLADVLVQSFHSASELKLGVVTSLLGAPFFLAIVLGARSRIT
                     "
     gene            26687..27460
                     /locus_tag="PLW13_03760"
     CDS             26687..27460
                     /locus_tag="PLW13_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69517.1"
                     /translation="MSTLALEGVSVRLGGRAVLQELSCALAAGEMLGIIGPNGAGKST
                     LLRVMAGLLRPDAGQVRLQGRELQSWPAAERARRIGYLPQAAPVHWPLDVRTVVELGR
                     LPWRHGWFSVDEGARDAVQAALRDAEVEELQGRLVNELSGGERTRVMIARLLAGAPGI
                     ILADEPVAALDAYHQLHAMEVLAERAARGTAVAVVLHDLSLAARFCQRILLLDHGGVA
                     AHGAARDILCPGVLEPVYGVRIRVLDEDGLVAIIPWQRR"
     gene            27511..28098
                     /locus_tag="PLW13_03765"
     CDS             27511..28098
                     /locus_tag="PLW13_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQY69518.1"
                     /translation="MNQPSSAIATAVAVTLPEWVPGVVDAFPACTNDTGRMRLAITLA
                     RENVERASGGPFGAAIFARGAPRPLAVGVNCVERLRNAVLHAEIVALMLAEARLGSYT
                     LRAPDAPEYELFTSCEPCAMCLGATLWSGVRRIVCAAGREDAMALGFEEGPVFPETWR
                     YLQAQGIALEHGLLAAEAAEVMRRYREGNGLVYNG"
BASE COUNT         4184 a         9435 c        10030 g         4549 t
ORIGIN      
        1 taatggcttt tgcgacaccc tctgaagccc gacctactcc gacctacacc aacctacagc
       61 tcctcgcagc agcggttgac ctccacgacc acgtgcgcca gcagcgggat ctcccgtagc
      121 agtgccttgt agtgttccgg cggccgcgga tcgtgtgtca ccacggaaag gctggcggcg
      181 taatggcgcg gcccgagcgg ccacacgtgc aggtcggcga tgcggttgtc ggcgtcggat
      241 tcgatgacgc gcgtgatcgc ggcctgggtc ccctcgtcgg cggccgcgtc cagcaggatg
      301 tggccgctgt cgcgcaacag gccccaggcc cagcgcgcga tcacgagcgc gccgatgatc
      361 ccgaccacgg ggtccagcca ggtccagccc acgtacttgc ctgcgagcag cgccacgatc
      421 gcgaacaacg aggtgagcgc atcggcgagc acatggaagt aggccgcgcg caggttgtgg
      481 tcgtggtgac catggtcatg gtcatggtcc tccgcgcgat catggtcgtg gtgatggtcg
      541 tgcagcatcg cgccgcacac caggttgacc acgaggccgg ccacggcgac gccgatggcc
      601 tggctgtagt gaatcgcctg cggctcgatc agtcgcatcc ccgattccag tgccatcatg
      661 agcgcgacca tggccagcgc caccgcgctg gcgaagccgc cgagcacgcc gaccttgccg
      721 gtaccgtagc tgtagcgcgg gttcgtcgcg tggatgcgcg cgtagcgata ggcgaacagc
      781 gtgatgccga aggcggcgac atgcgtggcc atgtgccagc cgtcggccag cagcgccatc
      841 gagccgctgg ccgtgccggc cacgatctcg atcaccatca tcaccgcggt gagcgcaatc
      901 acgcgccgcg tgctggtctc ggcgcggtcg gtgtggccgc cgaaatcgtg gctgtggcgc
      961 cagccgttca tgtcgtggtc gtgcattggc aggggtggtc cgggtccgtt cggggcgaaa
     1021 gtatacgcaa ggatgcgcgg gccgatgcga cgagtcgccg ccggcgcacg acgcgcgtgt
     1081 tgcgggaaat ccgcgggggc gcgcttcagc gcggcgtggc gcgttgccct tgccgcggac
     1141 tgttgcgcaa cggcgcaagc agttccttca gcccgttgtg gtcgatctcg tgcatgagcg
     1201 ccagcagccg gccgatctcg cccggcggga agccctggcg cgcgaaccag gcgaggtagt
     1261 tgccgggcag gtcggcgatc agccggccct ggtacttgcc gaagggcatc ggcagcgtca
     1321 ccagcttgcg cagggattcg ctgtccatgt tcatcccggg gccagcgctc gcgtgaggaa
     1381 gggcaggctg cgctccaggc ggtaatcgat gccggagtgg gtgccgtcga attcctcgta
     1441 ggtgtgcacg atgccgtggc gcgccagctc gcgcgacagc tggcggcagc cgtagtggat
     1501 gtggaactgg tcgcgccagc cgcagtcgat gaagatgccg cgcatcgacg ccaggttcgc
     1561 gcggtgcgcg gcgaccatgt ggatcgggtc gtgcgccagc cagcgctgcc agcgcgcctc
     1621 gatcaactcg ccggtctcga ggtgaaaggg caggcgaaag ccgttgggcg cgcgcgggtc
     1681 cgggtcgtag ctcgcggcca tcgcgagatt catcaggcac atggtctccg cgtgcccggg
     1741 ccggtcggtt ttccagatgt gctccagaaa gcggcgcacg cggccatcat ccaggccggg
     1801 cttcagtgca gcgctggcct tttcagtgtc gtaccggcct gccgtgcgcg ccgggcggcg
     1861 gtatttcgcc agttcgtcca gcgtgttcgg ccagtcggcg cggtagagga agtcgaagta
     1921 ggcatcgccc gagtggttcg ccaccgcgcc ccagtgcccg gcgtatttca tcgcgtgcac
     1981 gatcgcgccg tagccgcccg aggacttgcc gaaacagccg cgcgaatcgc ggctggcgcg
     2041 ggtgcggaat tcctggtcga tggcggggat cagttcgcgc gtgaggtagt cggcgtagcg
     2101 cccgatcgcg ctcgagttga tgtactggtt gccgccgagc gcggtgaaac agtcgggaaa
     2161 caccacgatc gccggcttca tgcgcttctg gtgcaccagg cgcgccagcc gctcgggcac
     2221 gttctcctcg aaaccgcgcc aggcgaggtg tgccggcccc gagcccatga accccgcgag
     2281 atcgaacagc acggggaagc gccggcgcgg gccggaggcg tggtattgcg ggggcagcca
     2341 ggcgtgcagc ttgcgcacgt gggggtcgcc gagcgggttg tcccgcagca ccgtggagac
     2401 gtgctcgagc gtgacgagct ggccctcggg ccagttgctg cgtttcaacg acatggctcg
     2461 attcctcctg ccgggctgcg cccgatgtta ttgccagctg aagcgcggct gggcgaagtg
     2521 gccgacgcgg gtgtggcgcc cggcgaaggc gacctcgcgg aactggtaga ggatggcgcc
     2581 ggtcggggcg tgcagttcgt cgccgatcgt cggcaggcgc aggctgatgg cgggctgctc
     2641 gctgtgcggc accgggtgca gggtcgggag cgcgggcgac gcgagctcgg ccagcgggat
     2701 gcggtagatc cgggcaacct cgcgcggatc cggcgccagg ccggcctcgt cctcgatcca
     2761 cagcacgaag ggccgcatgc gaaagcccga ctgcgtggcg tagtcatcga gcgtgcccag
     2821 gatgtcggag cgcgcggcgc tgatgccgat ttcctcgtgc agttcgcgca acgccgcgtc
     2881 ggcctcgctc tcgccgggat cgacgcgccc gcccggcaat gcgaactggc ccgcgtggcg
     2941 cttgatcgtc tcggggcgcc gggtcaacag gcaggcggcc tctccgtgcg cgccggcgac
     3001 gatggcgatc accacggcgg cgcgccgcag ttcttcgggg ctgaggatgt gcggggtgaa
     3061 agtatcgagg cgggcggcga tggacgagcg caacgcgtcg ccgaacggca ggccgccgtt
     3121 caggcgctca tcgcgagact caggcggggc cgcgcggggc tgtaacgttc caggcattca
     3181 ctggccttgt cgcggcattt gccgcagcag gtaccgacca gcagcgtgtc ttcgagatgc
     3241 gccatggtcg cggcgccgtt gtacacggca tccgcgatct cgctgtcggt cacggcattg
     3301 cagatacaga cgtacatcga ggcctcccgg gggagaagat gcgaacgatg gcgtcatgct
     3361 aggtgaaatg agagtgattc gcaatacaaa aacattacag atccgtagcg cattccgcgc
     3421 cgccggagtg cttacatctt cgactgcagg tagttctcga ttccgacctt gccgatcagc
     3481 tcgagttggg tctcgatcca gtcgatgtgc tcctcctcgg actcgaggat gtcctcgaac
     3541 agttcgcgcg agacatagtc gcgcaccgat tcgctgtgcg cgatggcctc gcgcagcacc
     3601 ggcaccgcct ccagttcgag cgccaggtcg gcctcgagga tctcgcgggt gttctcgccg
     3661 atgcgcagcg tgccgaggtc ctgcaggttc ggcaggccct cgaggaacag gatgcgctcg
     3721 atcagccggt cggcatgctt catctcgtcg atcgattcgt ggtactcgtg ctcgtcgagt
     3781 tgcttgaggc cccagttctt gtacatgcgc gcatgcagga agtactggtt gattgcggtg
     3841 agctcctggc ggaggacctt gttgaggaac tggatgacct gcttgtcgcc tttcattgcg
     3901 ggaaactccg tgagctggaa attgaggggc atgctaccag ttttgtcgcg acgctaccgc
     3961 cgccggttgg ctcagagccc gcgttcgagg atgccgcgca ggccgctcac gtcatagccg
     4021 gcctgtgcgg cggcggcgat caccgcgtcg gcgttgccgc cgccggcctg cgccagcgcc
     4081 cacagcgtgg tcgagcgcgt gccgctgcgg cagaaggcca gcacgggttg ttccgcgtgc
     4141 gccagcagcg cgcggaaggc gtgcacgtcg cgctcggtca gtgcgccgga caccacggga
     4201 aggtggtggt attcgagtcc ctgctcgcgc gccgcagcct ccagctcccg cgccgaaggt
     4261 tgcaggaagt cctccccgtc gggccggttg ttgatcagcg tcctgaagcc gagccgcgcg
     4321 gcgtcggcga tttcccgcac cgagagctgc ggagcgacgc tgacagcgtc gctgagcttg
     4381 cgcacatcca tgcgtgaatc ctgtggcgag gcgatgaatc ggaggcgcac agcatacggt
     4441 gcgcggcggc gcgacggaac agcgtcatgg tccgctgcga cggcttgccg gcaggcgggc
     4501 gcttgacgcg aactaccatt catttcggaa taaggtttca tctttttatt atttactaaa
     4561 ttagccgagc gaccttaagc tgcgcgcttt tttggtgcgg cggaatccgc accgaccggc
     4621 tgaaaggaag taccgcatgt cctctgttcg agttctgctc cgcgccagcc tgacccttgc
     4681 catcgccggc gggtccgcgg cgcacgccac caacggctac ctgtcgcacg gttatggcat
     4741 caaggccaag ggtgcggccg gcgtgggcgc cgcgctgccc caggacacgc tcacgatcgc
     4801 gaccaatccc gccggcttga tcgaggcggg tcaccgttcg gacttgggag tggagctctt
     4861 cgtgccggat cgcggcggcg agatcaaggg caatgccttc ggtccggacc agtccttcga
     4921 cggcaacggc accgagtact tcctcattcc cgagctgggc cacagccacc tggtctgcga
     4981 cgacctcgcg gtgggttttg cgatgttcgg caacggcggc atgaacaccg attacgacga
     5041 aaacccttac gcgcgctttg gcagcaaggg caagagttac atgaatttcg agcagctgtt
     5101 catcgcgccg gcattggcgt gggaattcag ccccggccag acgatcgggc tcgcgctcaa
     5161 cctggcctac cagcgcttcg aggcgaaggg cttgtctgcc ttcgcgccgc tctcggccga
     5221 tccggcgaac ctcgagcaca acggcaccga cgacgggttc ggtgccggtg tgcgcatcgg
     5281 ctggctcggg caaatcaccg accgcctgac gctcggtgcc agctggcagt cgacgacctg
     5341 gatgcaggaa ttcgacaagt acgccgggct gttcgccgag cagggtggct tcgacgtgcc
     5401 cgaaaactac gtactcggcg cggcattcaa actcagcgac gccaccaccg tcgcgctcga
     5461 ctggcagacc atcaagtaca acgatgtcgc ttcggtgggc aacgctctgg gccagctgtt
     5521 tctgggaaac ccgctcggca gcgacaacgg cccgggtttc ggctggcagg acatgagtac
     5581 gctgaaaatt gccgtgctgc accagtactc ggatcgcgtc acgctgcgcg caggctacag
     5641 tcagggcgac cagccggttc cgcgcgacca gaccttcttc aatatcatcg cgcccggcgt
     5701 ggtcgaagag cacgtgtcgc tgggtgccac ctttgccttc aacgagcgca acgagctcag
     5761 catcgcctac acgcgggggc tggagcacga ggtgcatggc aagggttcca tcccggcgat
     5821 gctcggcggc ggcgaggcca acatctcgct ggcggaggat tccttcggcc tggcgtggtc
     5881 gctaatatat tgacactcga gtatttccaa aggtttctga cgcatgactg acttggttca
     5941 cctggtggcc ggcggcgccc tcatcggcgc cggctcggcc gtgctcgcct tggccaacgg
     6001 ccgtatcgcc ggcatcagtg gcgccttcgg caacctgctc gacggcgacg ccggtccgca
     6061 gcgctggcgg ctcggcttcc tgctggggct gctgctgccc gcgctctacc tgctgctgaa
     6121 cggggtggat gtggcgatgg ccggcggcgt ggcctggctg ctcgtggccg ggttgctggt
     6181 gggcatcggc acgcgcgtcg gctcgggctg cactagcggt cacggggtgt gcggcaccgc
     6241 gaatctctcg aagcgatcga tcgtggccac gctgctcttc atgagtgcgg cgatggctac
     6301 cgtctacgtc gtgcgtcacg tggtgcaggg gtgaagccga tgcgtattcc cgccttcctc
     6361 tccgccctcg tcgccggcct gctgttcggt acgggattga ttgtttcggg catgacggac
     6421 ccgcgaaacg tgctggcttt cctcgatatc gccggtaact ggaaccccgg gctggcggtg
     6481 gtgatggcaa cggccatcgc gatcaccgcg cccgcgttgt ggctcctgaa gaagcgaggc
     6541 aaaacgctcg ccggcgagag ctgcgacatc gccaaccgca acccgatcga cacgtcgctg
     6601 gtcgtgggcg cggtggtgtt cggcatcggc tggggcatgt cgggcatctg cccgggaccg
     6661 gggctgatca tcgccgtcgg cggcgtgcct ggcgcgctgc tgttcgtcgc cgcgatggct
     6721 gccggcatgc gcctcagcgg catcgtgctg cgccgcctgg gctccagcgc cgccccctga
     6781 cacccgagcg ggtcccgaac agggtccccg cgcaatgagg agttacctgg cgagggcgcc
     6841 gagggcgagc ttcagcagcg ggtcggcgac gcggtcgggc agggcactca gtgcaacgcg
     6901 ttgcgggtcg ggcttcaccg agtaggcgat gcgcgggtag cgcgtcgtca gggcggtgtg
     6961 cacgacttcc gccagcagcg cgggatcgtg cgccgtgccc tgttccctga cggtcgcttt
     7021 cttgagcgtc tgcagcagct ccttgaagta gcgcgaggct tgcgcgacgc gctcgaagtt
     7081 ctgctcgatg ctggcaacca tcccggtgcg gaacggcccc ggctggatct tgatcaccgg
     7141 cacgtcgagg aacatcagtt ctcgccgcag tgcgtcggag taggcctcga tcgcgtgctt
     7201 gctgagcgca taggcgccgt tgaagggcat cgcgctctgc cagccggtct ctgagctgat
     7261 gttgacgatg cggcccttgc gcgccagcag cagcggaaac agcgcgtggt tcacgcgcac
     7321 cgtgcccatc acgttgacgt cgagcacgcg gcgtatcgta tcggggtcga tctcgatcac
     7381 cgagcccacc gcgaggatgc ccgcgaagtt cacgatgccg tccagcccgt cacaggtacg
     7441 cgccacgcgc cggcgcgccg cctcgcagct cgtggcgtcg gtcacgtcga gttgcaccgg
     7501 gatgacgtcg gtttccgcgg ccagcgcctt cagagccctg gcatcgaaat cggccgcgaa
     7561 cacggtccag tcccgctctg ccaggtagcg cgcggtagcg ccgccaaggc cgccgcccgc
     7621 gccggtgatg aatacggttt tcgtgtcgct gcgtttgcgg gccatgccgc acctccctga
     7681 tttcaacaaa ctcgtaaaaa tcttacgcct cccggcgctg ccggaacttg gcctggatca
     7741 tcaaagcggg cggagggatc aaatttcgat caatcggcgc tgtgttacca tgccgcgtca
     7801 tttttcgacg cgttccaaga ccatgcgctg ccccttctgt tccgccgccg acaccaaggt
     7861 catcgattca cgcctggtcg cggaaggcga ccaggtgcgg cgccggcgcg aatgcctgag
     7921 ctgcgcggag cgcttcacca cctacgagac ggccgaactg ctgctgccgc gcatcatcaa
     7981 gcagagtggc gtgcgcgagc cgttcaacga ggacaagctg cgccagggca tcctgcgcgc
     8041 gatcgagaag cgtcccgtca gcatggagcg cgtcgagctg atgctgagcc agatcaagca
     8101 tgcgctgcag gcgacgggcg agcgcgaagt cgcctcgcgg gtcgtgggag agcaggtgat
     8161 gcgtcagctg aaggcggtcg atcacgtcgc tttcgtgcga tttgcttccg tctatcgcag
     8221 cttccaggac ctgaacgaat tccgcgccga gatcgagcgc ctggccgagg agcaggacag
     8281 tccgccgccg ggcgcagcgg gctagcctgc ggccatgagt cagcagatca ccgacgtgcg
     8341 ccacatggcg caggccatcg ggctcgcgcg ccgcggcctg tgcaccaccg cgcccaaccc
     8401 gcgcgtgggc tgcgtgatcg cgcagggcga gcgcgtgata gcgagcggct tccaccgtcg
     8461 tgccggtcag gcacatgccg aggtcgaggc gctgcgcgcg gcgcgcgagc cggtgcgcgg
     8521 ggcgaccgcc tacgtgaccc tggagccctg cagccaccac ggccgcacgc cgccctgcgc
     8581 cgatgcgctg atcgatgtcg gtgtcgcgcg cgtggtttcg gcctcgctcg atccgaaccc
     8641 gctggtcgcc ggaagcggcc tggataggct gcgcgcggcc ggcatcgcgg tcgaatccgg
     8701 catcctcgag cgcgaggcga tggcgctgaa cccgggcttc ttcaagcgca tgagaaccgg
     8761 gatgccttgg gtgcgcgtaa aactggcaat gagtctggac ggccgcaccg cgatggccga
     8821 tggcagcagc aagtggatca ccgcggatgc ggcgcgcgcc gatgtgcagc gcctgcgcgc
     8881 gcgcagctgc gcgatcgtga ccggcgtcgg taccgtgctg cacgacgacc cgcgcctgga
     8941 tgtgcgtatc gcgacggatg acggcgtgac gcccgggcgc cagccgctgc gcgtggtggt
     9001 ggattcgcag ctgcgcatgc cgccctcggc gcgcgtgatc ggcgcggccg gtgacacgct
     9061 cgtcgccacc gccgggcgcg atgccgcccg cgagcaggcg ctcgccgatg ccggcgtgac
     9121 actgctgcat ttgccggccc cggacgggcg cgtcgacctg cgcgcgctgc tcgcgcacct
     9181 tgccgtggcg cagtgcaacg aagtgctgat cgaggccggc gcaacgctcg cgggcgccgc
     9241 gctgcgcgcc gggctggtcg atgagctgat catttacatg gcgcccacgc tgatgggcag
     9301 cgcggcgcgc ccgctgctgg acctgccgtt cgcgtcgatg gacgagaaac tcgggctgca
     9361 gatcagcgat atcacggccg tgggcagcga ctggcggatc acggcgagcg tgggcggggc
     9421 gtaggtcggg cgtacgtcgt gcgtaggtcg ggcttcagcc cgacatccaa ccgacggttt
     9481 cgactggaac aggattgaaa cgaatgttcg gctcaagccg gacccacaag cgcagatgga
     9541 ggcgcgagcc gcgcgcccac ctgcaatgta cgattgacgc ggaaggtcag gtaccaatgt
     9601 tcacaggaat catccaggcc accggcgcgg tgcgccacct cgagcgccgc ggcggcgacg
     9661 tgcggctgca cgtcggcacc ggcaagctcg aactcggcga cgtgcgcgcg ggagacagca
     9721 tcgcgaccag cggcgtgtgc ctcacggtgg tggagttcgg caccgactgg ttcgcggccg
     9781 acgtttcgct ggaaacgctg gggctcacca cgctcggcgg cctcgcggcg ggcgcgcgcg
     9841 tcaacctgga aaaggcgatg gcggtgggtg agcgcctcgg cgggcatatc gtcagcggcc
     9901 atgtcgatgg cgtcggcgaa gtgctttcga tgacgcccga cggacgttcc acgcgctaca
     9961 gcctgcgcgc gcccgcggcg ctggcgcgct acatcgcgca caagggctcg atctgcgtcg
    10021 acggcgtgag cctcacggtg aacagcgtcg acggcgccgg gttcgagctg aacatcatcc
    10081 cggtgacggc gcacgagacc atcttcggcg actaccgtcc cggcacgcgc gtgaacctcg
    10141 aggtcgacgt gatcgcgcgc tacctcgaac gcctgctgca gggcgatgcg gccgcccact
    10201 ccgggtccgc gcaccgcggc gtcacgcgtg aactcctggc ccagaacgga ttccccggaa
    10261 catgaaattc gacaccaccc agcaactgat cgacagcttc cgccacggcg acatggtggt
    10321 gctgctcgac gacgacgacg aaaacctcgg cggcgtgctg ctcgctcccg cgcaggacat
    10381 cacggccgag acggtcaact tcatggcgcg ccaggcccgc gggctgatct gcctgagcct
    10441 cacgggcgaa cgctgcgcgc agttgcgcct gccgctgatg gtcgagagcg cttcgcacag
    10501 cgtctacgcc agccggttca cggtctccat agaggctgcc gaaggcatca gtaccggcat
    10561 ttccgccgcc gaccgcgcgc atacgatacg taccgccgtg gcgcgcggcg cgcagccgcg
    10621 cgacctggta cagcccgggc atgtgtttcc attgcgcgcc gagccaggcg gggtgctcaa
    10681 gcgcgccggc cacaccgagg gcggctgtga cctcgcgcgc ctcgcgggct tcgagcccgc
    10741 cgcggtgctg gccgacgtgc tgaacgacga cggcacgctg gctaccggac aacgcctgct
    10801 ggccttcgcg cgccagcacg gcctgcgcat tggcaccatc gcggggctga tccagttccg
    10861 cctgatcaac gagaccacgg tgcaccgctt ccgcgagggc gaggtgcaga cagccttcgg
    10921 tgcttttcgc ctgcacgcct ttcgcgacgc cgacgacggg cgcctgcacg tcgcgctggt
    10981 gcgcggcgcc atcgatccgg acgcgccgac gctggtgcgc gtgcaacagg cggccacgct
    11041 gcgcgacgtg ctcggcacgg aggtgccggg acgcaagccg aactggaacg tctcgcgctg
    11101 cctggaatgc atcgaccggg agggccgcgg cgtgatcgtg ctgctgagcc agcccgaaac
    11161 cgagcagcag ctgctggcca gcgtggacgc ggctctcggc ggcgaggcgg caaccgagcc
    11221 gacggcaccc gagggcggac agagcgtgct gaacctggtc ggcgtgggct cgcagatcct
    11281 gcgccagctc ggcgtggggc agatgcgggt catgggcccg ccgatacgct acaacgcgat
    11341 ctcgggcttc ggtctcgagg tggcggagta cgtcatgtac tgagcggccc gcgcgcggcg
    11401 atggccgcag caggcgtttg atggcagaat tggcgccctt tcgcgctaca acccacaggc
    11461 aggacgatgg ctgagatcag gacaatcgag ggagtcttcg cggcgggaga ggcgcgtttc
    11521 gcgctggtcg ccagccgctt caacagtttc gtggtggaga gcctgatcgc cggtgccgtc
    11581 gatacgctgc gtcgtcatgg cgtagccgac gcgcagatca cgctggtgcg ctcgcccggc
    11641 gccttcgagc tgccgctggt ggtgcagaag gtcgcggcga gcggtaactt cgacgcgatc
    11701 gtcgcgctcg gcgcggtgat ccgcggcggc acgccgcact tcgagtacgt cgcgggcgag
    11761 tgcaccaagg gcatcgcgca ggtgtcgctc gcgagcggtc tgccgatcgc cttcggcgtg
    11821 ctgaccgtgg ataccatcga gcaggccatc gagcgcgccg gcaccaaggc cggcaacaag
    11881 ggcgcggaag cggccctgtc ggcgctcgag atggtcagcc tgctcggccg gttgtgagcg
    11941 cgggtgtcgg cttgagcgac aacgcgctcg aaaacgaacg tcgccagctc gcggcagcgc
    12001 ggcgcaaggc gcgccattat gcggtgcagg cgctgtacca gtgggcgatg accggcaatt
    12061 acctggccga catcgagcag catttcaccg aggaattcga tttccgcggc gcggacacgg
    12121 agtacttccg tgaactgctg catggtgtgc cggccgatct ctccgcgatc gaggccgcga
    12181 tggagcccta cctcgacatt gccctcgaaa agctcggccc ggtcgagcgc gcggtgctgc
    12241 gcctcgcggt gtgggagctc aagagccgtc ccgacgtgcc ctatcgcgtg gtgctcagcg
    12301 aagccgtcgc gctggcgaag aaattcggcg ccgccgagag ccaccgcttc gtcaacggtg
    12361 tgctcgacaa ggtcgcgcgc gacctgcgtc ccgcggaagc cggccgcgcg cgctgagccc
    12421 gggtccatag ccgtggcgct gtccgaattc gaactgatcg cgcgctactt cgattctcag
    12481 gacttcgcgc tgcccgaggc cgagggcgtg ctgctcggca tcggtgacga cgccgcgatc
    12541 ctccggttgc cgccgggcga ggatctcgcg gtctccatcg acacgctggt cgccggcgtg
    12601 cattttcccg ccgccagcag cccggagcag atcggccacc gcgcgctggc ggtcaacctc
    12661 agcgacctgg ccgcgatggg tgcacgcccg ctgtggttca cgctcgcgct gaccctgccc
    12721 gaggccgatg agacctggct cgccgggttc agccgcggcc ttgccgcgct ggcgcggcgc
    12781 caccatgtcg cgctggtcgg cggcgacacc acgcgcggac cgctcagcat caccatccag
    12841 gtccacggct gcgtgccgcc cgggctcgcg ctgcgccgcg atggcgcgcg cgcgggcgac
    12901 gacgtctggg tcagcggcca tccgggcacc gcggcgctgg gcctgcgaca actcctggcg
    12961 ggcggcgcgc gcgacgaggc ggcgcgggtg ttcgcggcgc cggcgccgcg ggtcgcgctg
    13021 gggctcgcgt tgcgcgggct cgcctcggcc gcgatcgatg tctcggacgg cctgctcgcc
    13081 gacgccggcc atatcgccgc gcgcagcggg gtggcgatcg cgctggagcg cgagcgcctg
    13141 ccgttctccc cgcaactgga gtcactcgcc gatcgcgacg acgcgtgcgc gctcgcactg
    13201 ggcggcggcg acgattacga gctctgcttc acggcgccgc cacgccatgg cgcggccatt
    13261 gccggcgccg ccgcggacct cgggctcgcg tgcacgcgca tcggccgtgt gctggcgggg
    13321 cagggcgcga cgtgcgcggg tttcgtgccg cgtacgggcg gttaccagca tttccgtggt
    13381 gcctgaagcc atgcgctccc gcgtcggccg caggcggacc ccggtgctgc gacgctggat
    13441 gctggccgcg acgctgctgc cgctcgccgt agcggcagag tccctgcacg tcacggcagc
    13501 ggcgctgttc gagggccgcg cgatgctcgg catcgacggc agccggcgcc tgctgcgcgt
    13561 gggcgagacc tcgccggaag gcgtgaagct ggtcagtgcc actgcgcgcg aggcggtgat
    13621 cgagatcgac ggccggcgcc agacgctcgc gccggggcgc gacaccggcg gcggctttgc
    13681 cgcgcccgag cgcaagcgcg tcgcgatcca gcgcggcgcc cggcatgaat acctggtggc
    13741 aggtaccgtg aacggactgc aggtgcagtt ccttgtcgat accggcgcga acgtcgttgc
    13801 gatgaacggc gccgacgcgc gccgactcgg tgtcgattac cgcctgcacg gcaggccggg
    13861 tgcggtgcag accgccagcg gggtcgtgca ggcctggtcg gtcacgctgg atcgcgtcga
    13921 ggtcgcgggg atcgcggtgc gcaacgtggc ggcgtcggtg atcgagggca actaccccgc
    13981 gcagctgctg ctcggcatgt cgtggctcgg acgcgtcacg atgcgtgaag agcacggtgt
    14041 gctctatctc gaggaaaaat agccgcgcct cgcgctccat tgtcacgcgc gtgacacgct
    14101 ggtgtgagaa atgcgggcca gctgctagaa tcggcgcccc ttgagccggc cggagccgcc
    14161 cgtgtctgtc cgttatatcg cctcgtcacg cttgccgaca ccctgggggg tgttcgacat
    14221 gcatggcttc gaggatatcg agagcgagaa ggagcacgtg gtcctcacca tgggtgatgt
    14281 cagcaacggc gaacccgtgc tggcgcggat ccattcggaa tgcctcaccg gagacgcgct
    14341 gttcagcatg cgctgcgatt gcggcgcgca gttgcgcacg gcgctggagc gcatcgcgcg
    14401 cgaagggcgc ggcgcgctgc tctacctgcg ccaggaggga cgcggcatcg ggttgttgaa
    14461 caagatcaag gcctatcgcc tgcaggacgc gggcgcggat accgtcgagg ccaacgagca
    14521 actcggtttt ggcgccgaca tgcgcgacta cagcatctgc cgcacgatgc tcaagcatct
    14581 gcacatcaca tcgctgcggt tgatgaccaa caacccgcgc aaggtcgcgg cgatcgagga
    14641 catggggatc aaggtcgtgg agcggatctc gatccgcacc ggacagaacc cccacaacgt
    14701 gcgctacctc gaaaccaagg ccggcaagct cggacactgg atggacgagg atcctggcgg
    14761 ctgagattgc gggtcaggct gtaggtccgg atttatccgg acgattgttc ggctgaagcc
    14821 gaacctacag ctggctcacg gcgctccagc gcgagggccg catcgccgcc aggcgctcgc
    14881 ggatggcggc ctcgatgccc tcggcatcca gtcccacgtc gtgcagcatt tgctgcggct
    14941 tgccatgctc caggaaacgg tcggggagcc cgagcacgag cagcggcatc acgatgccgc
    15001 gcgcggccag caactcggcc accgcgccgc ccgcgccgcc ggccacggca ttctcctcca
    15061 ccgtgaccag cagcgaatgg ctggcgcaca gttccaggat caactcctcg tccagcggct
    15121 tgacgaagcg catgtcggcg acgcttgcct ccagtgcgct cgctgctgcc aacgccggcg
    15181 cgaccatgct gccgaaaccg aggatcgcta cctcgcgtcc ggcgcggcgc agcacaccct
    15241 tgccgattgc cactgcctcc agcgtcgggt cgatcgtggc gccagggccg gtgccgcgcg
    15301 ggtagcgcac cgcggcgggt ccgtggtgca ggaagcccgt cgagaggagc ttgcgcgtct
    15361 cgtcctcgtc ggagggcgcc atcaccacca tgttgggtac gcagcgcagg tagccgaggt
    15421 cgaaactgcc ggcgtgcgtg gggccgtcct cgccgaccag cccggcacga tcgatcgcga
    15481 acagcacgtc gaggttctgc aacgccacgt cgtggaccag ctggtcgtag ccgcgctgca
    15541 ggaacgtcga gtagatcgcc accaccggct tcatgttctc gcaggcgagg ccggcggcca
    15601 gcgtcaccgc gtgctgctcg gcgatcgcga cgtcgtgaaa gcgcgccggg aagcggcgcg
    15661 cgaactccac catgcccgag ccttcgcaca tcgccggcgt gatgccgacc aggcgctcgt
    15721 cgcgccaggc catgtcgcac agccagtcgc cgaagacttc ctggtacttc gggcgtaccg
    15781 ggacgggctt ggtgccggcg acgcgcggcg ccgggtcgat cttgttgatc gcgtggtagc
    15841 ccaccgggtc gcgctcggcc ggcgcgaagc ccttgccctt taccgtcatc acgtgcagca
    15901 gctgcgcgcc tttcagttcg cgcacgttgc gcagcgtggc gagcagcgtc gggaggtcat
    15961 ggccgtcgac ggggccgata tagttgaagc cgagctcctc gaacagggtg cccggcgcga
    16021 ccatgccctt catgtgttcc tcggtgcgcc gcgcgatctc gagcgccggc cccggcaggc
    16081 gcgacagcac gcgcttgccg gcctcgcgca tgtggttgta cgagcggctg gcccagatgc
    16141 gcgcgaagta ggaggccagc ccgccttcgt tgcgcgagat cgacatctgg ttgtcgttca
    16201 ggatcaccag aaggtcagca cccgtgtgcg aggcgtggtt cagcgcctcg aaggccatgc
    16261 ctgccgtcat cgcgccgtcg ccgatgatcg cgaccgccct gcggtcctcg ccgtgcgcgc
    16321 gcgccgccag cgccatgccg agcgcggcac tgatgctggt gctggaatga ccgacgccga
    16381 agcagtcgta ctcgctctcg gcgcggttgt tgaatgccgc caggccgccg gccttgcgga
    16441 tcgtcagcat cgcctcgcgc cgccccgtca ggatcttgtg cgggtagctc tggtggccca
    16501 cgtcccagac cagtcggtca cgcggggtgt caaacaggta atgcagcgcg atgctgagct
    16561 cgaccacgcc gaggccggcg ccgaaatgcc cgccggtctg gcccacggtg tagagcagga
    16621 attcgcgcag ctcgttggcc agctgcagca gctgggcctc gtcgagctgg cgcaggtcgg
    16681 cgggaaatgt cacgcgatcg agcagcggcg tcgccgggcg cgtcacggga atttcctgga
    16741 acaacttcat gcgttgggct ttcctgcggg agtgccggtg tgctccggcc gttcgaggcc
    16801 gcgattctaa cgcatccggc gggttgcccg taatcggcgc tcagcgatcc cggctcacga
    16861 tgaaacgcgc catttccgcc agtgccgccg cgggcccgcc gaggggcgcc agcgtcgcca
    16921 gtgccgcatc gcgcagctcg agggcaagcg cgcgggcgcc ggcctcgccg agctcggtga
    16981 cataggtcgg cttggtctgc gcggcgtcgg aacccgggtt cttgccgagc gccgccacgt
    17041 cgccgcaggc gtcgaggatg tcgtcctgca cctggaaggc gagtccgatg cagcgcgcgt
    17101 actcgtccag gcaggcgcga gcgcgcacgt ccgccgcccc gagcagcgcg cccatgcgca
    17161 gtgccgcgcg gatcagcgcg ccggtcttca gcgcgtgcat ggactccagc gtggccgccg
    17221 tgatctggcg cccgacatgg gcgacgtcga ccgcctggcc gccgaccatg ccacgactgc
    17281 cgctcgcgct ggccagttcc gcgaccagcg cgagccgcgt ggcggcatcc accgagtcgg
    17341 cggcgcacaa caactcgaag cccagtgtct gcagggcatc gccggcgagg atggcggtgg
    17401 cctcatcgaa agccctgtga caggtgggct gcccgcggcg caggtcgtcg ttgtccatgg
    17461 ccggcaggtc gtcgtggatc agcgagtagg tgtggatgca ttcgatagcg cacgcggcgc
    17521 gatcggtgtc cggggcactg gcacggctgc cgccgaccgc cgttgccgcc gcgtaaagca
    17581 gcaccggacg caggcgcttg ccgccggcca gcaggctgta gcgcatcgcc tcgcgcagcc
    17641 gagcatcggc gcggccgacc agttcgccgt cgaacagcgt cgccagcgcc gcctcggcgc
    17701 gcgcgcggta ggtctcgagg tgtgcgccgt tgaccggcac ctcagacacc ggcgtcctcg
    17761 tcggcgtcgt ctgcctcggc gaacggcgtg gcgtgcacct cgccgtcctg caccgagagc
    17821 agttgcacgc gctgctgtgc ctgggtgagg gcctgctggc actgctgggt caggcggatt
    17881 cccgcctcga aagccttcag cgattcctcc aggctcagtt ccccgtcctc catgcgctcg
    17941 accagcgcct ccaggtcggc cagggatttc tcgaagttca ggggcttgcg cggtgctgac
    18001 acggtgctcc ggcctgtgga ggagttgacg cgcgcgacag tagcccgagc ccccgcacaa
    18061 atcaacgtgt agttcgggct tgtaattcgg gcttcagccc gacaaggatt cggcgcagcc
    18121 gaacccgccg ggtcccggtg cgcacccctc ggcatgaatg ccgaccccgc cggggctgaa
    18181 gtcgaacccg caaaggcggc tagaatgcat gcagtgcgta gcgtgcgagg ggaagacgtg
    18241 gatctggcga cgataatcgg cctgctcggg gccctgctca tcatcatggc cgcgatattc
    18301 ctcggcggcg atccggtgat gttcctcgat gcgccctcgt tcctgatcgt gatcggcggc
    18361 accgtgttcg tcgtgatgag caagttcggc atgaaccagt tcgccggcgc cttcagggtg
    18421 gcgggcaagg ccttcctgtt ccgtcccgag gagtcggggg agctgatcgc gcgcatcgtc
    18481 gagctggcca acctggcgcg ccgcggcggc ctgctcgcgc tggaggagca gccggtctcg
    18541 aaccgcttcc tcgccgacgg cctgcgcctg ctgatcgacg gccacgacgc cgagcacgtg
    18601 caggccgcgc tgtcgaagga ccgcaggcag gcgatggaac gccacgaatg gggccagcgc
    18661 atcttcctcg cgatggccga cgtcggtccg gcaatgggca tgatcggcac gctggtcggc
    18721 ctggtgcaga tgctcgcgaa catggacgac cccaagtcca tcggcccggc gatggcggtg
    18781 gcgctgctca ccacgctcta cggctcgctg ctggccaacg tcgtctgcct gcccatcgcc
    18841 gacaagctga agctgcgcgg cgccgaggag gcgacgcagc acgcgctggt ggtcgacggg
    18901 ctgctggcga tgcaggcggg gcagaatccg cgcgtgatcc agggcttgct ggagcgctac
    18961 ctgcccgcag gccagcgtcc ggcccccgcc tgaggcatcc ggcgcatgga tgaggacgag
    19021 cagcgcggca gcacggagat cgccatcccg aaatggctgc cgaccttcgc cgacatgatg
    19081 acgctgctga tgtgcttctt cgtgctgctg ctcaccttcg ccgagatgga catcaagcgc
    19141 tacgaacaga tcgccggctc catgtcggaa gccttcggcg tgcagcgcag gatagtggcc
    19201 gagagcgtgc ccatgggcac cagcgtgatc gcactggaat tctcgcccgg cgtgccgcgg
    19261 cccacgccga tcagcgagcc ctaccggcag gacgatgcgc tgcccgagct ggcgcccgac
    19321 gccgcggcgg acgaagcgac gccggatctt gagttgacgg agcagatgaa gcggaacgtc
    19381 gaggcgcaga tccgtgccac gcaggccgat gcggccgcgc tggccgagaa gctcacgggc
    19441 gagatcgccc gcggcgaggt cgagatcgag accagcggac gtcgtatcgt ggtgcggatc
    19501 cgcgagcgcg gttccttcgc ctcgggttcc gcggagctca aaccggagta ccaggcgctg
    19561 atgcggcagt tccgcgacgt gctggccgcg caggagggag cggtcgaggt gcagggccac
    19621 accgatgatg tgccgatcgc gacggcgcgc tttcgctcca actgggagtt gtcgtcggcg
    19681 cgggcggtgt cggtcgccga gccgctgctc gagggcggcg tgctggaccc gcgccggctc
    19741 tcggtcaccg gctttgccga cgcgcgcccg ctggtggcca acgattctgc cgacgcacgc
    19801 gcgcgtaacc gtcgcgtcga gatcgtgatc gaccagggca tcgaccggca gctgcgcgag
    19861 cagctcgatg cgctgaagac cgtggatccc gcatactacc gctcgctgcg gctcgacgag
    19921 gccggggagg ggcggggcgg cgcggtgcac ggatcaggag gcggcagatg atcgagaagc
    19981 ggagcggcgc ggaccgcagg cgattctttc gcatcgacga ccggctgcgg ctcgccgtgc
    20041 gccgcgtgcc gggcgtcgat gcagtcgccg gtaaaggcca cgacctccgt ggcgacgagt
    20101 tcctggcggg tatcgaccgg ggcatagccg cgatcattgc cgccgcgcgg gtgcaggcgc
    20161 cggcggtggg cgagctcgcg caactgctga accgcaagct cgattacgtg atcgaacggc
    20221 tggggcagat cgagacgccg cgcgccggcc tgccgcttgt cgagcacgag gtcagcatca
    20281 gcgcctgcgg gattggcctg cagagcgccg agcgctacga ggcgggcgag acgctgcagg
    20341 tcgagctgat gctgccgccc gaggacaccc gcctgtgcgt gccggcgcgc gtggtgcgct
    20401 gccgcgacgc gacgcctggc aaccatctgc tgcagctcga ttttgccggc atggaccacg
    20461 acgaccagga actgctgatc cgcttcatcc tgcgccgcca ggggcagttt ctgcagggtc
    20521 tgcgcgagca gcgcgacgcg cggcgtgcgc gggtgagcgc ctgaggcaga cagcgcccgc
    20581 ggcgctgacg taacatcaaa tacttcatcc acgacccgga gaatcacggt gcgctacgga
    20641 atctgcctgc cctacatgaa gcgcgattac gatcgcgaca ccatcctcgc gtggtgccgc
    20701 gcgatcgatg ccggcccctg ggacagcctg tcgtgcggcg agcgcatcac cggatacacc
    20761 tgcgacatgc gcgtgctgct gtcggccgcg gcggcggtga ccgagcgggt gcggatagtg
    20821 ccctcgctgt acgtgctgcc gatgcattcc gcggtgtggg ccgccaagga aatcgccacg
    20881 ctggacgtgc tctcgggcgg tcgcgtcacc gtgaccgtcg gcgtcggcgg gcgcgagaag
    20941 gactaccgtg cagtgggcgc ctcgttcgcg caccgccacg agcgcatgga ccagcaggtc
    21001 gccgagatgc ggcgcatctg ggcgggcgag ccgccgttcg agggcgccga tccggtcggg
    21061 cccgcgccgg tgcaggccgg aggcccgccg atactgatcg gcgcgatggg cccgaagggt
    21121 atgcgccgtg gcgccgcgtg ggcgcagggc gtatacctgt tctcgatgaa cggcgacaag
    21181 ggcgagatgg cgaagatgat cgacatggcc gacagcgcgt gggaggaggc cggccgcgcc
    21241 gaccgcccgt accgcatggc cggcttctgg cactcgctcg ccgatgatgc cgagccgcgg
    21301 ctgcgccagt acgtctacga ctacctgaag atcgccggtg acgacgtcgc gaagctggtc
    21361 gccggcacca tgacgcgctc gaacgaggac gcggtgcgcc gcgcgatcga tgattaccgc
    21421 gagctcgagg tcgacgagat catgttttct gcggtgaccg cggatctcgc cgagctcgat
    21481 cgcctggcca atctcattgc caggatcggc tgatccctcg ccggtgtcgc ccgtagtccg
    21541 gcgtcagccg gacctgcggg acacatcaac tgcactggca ctgtccggct gaagccggac
    21601 ctacgctata ctgcgcgctc tcctgggggt gggtaacacc tgagacaccg gcatcgggct
    21661 ggtgaacccc tcgaacctga tccggataat gccggcgaag ggataggaaa acgcatgccg
    21721 cccggtgtgc gcgccaatat ccgccagcgc tggtccggat ctcctttacg gaggtcgcgg
    21781 atgcgtcacg tttcattttc gtctgcaggc agcgcgcgcc ggcgcgcgtt gtccctgtgc
    21841 cttgccgtcc tcggcgcgcc gcacgccggg gccgccgcgg cgcccgaacg gctcgaggag
    21901 atcgtggtca ccgcgcggct gctgcccaaa cccgtggata cggtcccggc gagcatcagc
    21961 gtgctgcccg cggcgctgat ccgcgagcgc caggccaacc atttcgagga gttgctgaac
    22021 gcggcgccga acgtgaacta cagttcgggc gcctcgcgcg ggcgcttcgt gcagatccgc
    22081 ggcatcggcg agcgcagcca gttcgtcgat ccggtcgatc cctcggtcgg gctgtacata
    22141 gacggcatcg atttcagcgg gctgggcaat gcgggcacgc tgttcgacgt cgagcaggtc
    22201 gaggtgttgc gcggcccgca gggcaccgcc ttcggcgcca gcgcgatggc cggcctggtc
    22261 aacatccgct cggcggcacc ggcagaccgg acgatggcgc gcgtggaagg cggcttggcc
    22321 gaatacggcg gcagcacgct gggcgcggtg gccagcggcc cgctgacggc cgacctcggc
    22381 gcgcggctcg cagtccaccg caaccgcagc gacggctaca tcgagaacga cttcctcggg
    22441 cgcgacgaca ccaacgccat cgacgagacc accgcgcgcg cacggctcga ctggcaggcc
    22501 ggcgaggcgc tggcgctcgg gctgacgctg atgcgcgtcg aagcggacaa cggctacgac
    22561 gcgtggtcgc tggacaacac ccgccacacg ttctcggacg aacccgggcg cgatcgccag
    22621 accagcgatg ctgctgcatt gcgtgccgac tggacgaccg gggcgggctt cgacgtgcag
    22681 gccgtcgcga cctggatggg ttcggacctc gactacggct acgacgagga ctggtcctac
    22741 gcgggcatct gcgacggcac cgactgcgag ggctgggagt actcctccac cgaccgttac
    22801 ctgcgcgacg tcgaggcggg cagcgtggac ctgcggctgg tgtcgcatcc gggcgagctc
    22861 acgtgggtcg ccgggctcta cgcggtcaat cgcgacacgc agctcgagcg ccgcttccat
    22921 gactgggacg tggatgcgcc gggactcttc cgcagcgctt acgacagcgg ccacgtcgcg
    22981 gcctatggcg agctcgactg ggcggcgagc gagcgcgtcg ccgtgagcgg cggactgcgg
    23041 ctgcagcgct tcgatgcgga ttacgacgat tcgctggggt tgcgcgagca gcccggcgac
    23101 gacctctggg gcgggcagct gagcgcgcaa tacgagctgg cgccgcgttc gatggcctat
    23161 gcgctggtcg cgcgcggcta caaggccggc ggcgtgaacg gcgaggcgat cggcaaggcg
    23221 cagaagtcgg gcctcgattc ctcgatcatc gccttcctcg gtacgcgcag cgagtacgcg
    23281 gccgagacgc tctggaacta cgagctcggc tggaagttcg cgagcaacga cgaggcattg
    23341 cgcgtgcgag cggcattgtt ctacatggac cgcgacgacg tgcagctgaa ggcctggtac
    23401 aacgaagggc cgcagttcgt gggctacacc gacaacgccg ccagcggcag caattacggg
    23461 ctcgaactcg aggccgactg gcaggtcttc gagcgcctgc gcctcgatgc ctcgctcggc
    23521 ctgctcgata ccgagatcgg gaatttcgtg gccaacgacc cggagctcgg cttcgtcgac
    23581 aaggacggcc gcgagcaggc gcaggcgccg aactggcagt tccgcgcggg tgccgagctc
    23641 gcgctgttcg cgcgcggctt cgcgcgggtc gaatacgagg gcaaggacgg ctactatctc
    23701 tcggactccg atgacgagcg cagcgaggcc taccggctgc tgcacctcgc cgccggttac
    23761 cgcggcgatc gcgtcgaggc cacggcttgg ctgcgcaacg cgctcgacga ggactacgtg
    23821 gtgcatggct tctatttcgg caacgacccg cgcacgttct acgtcaacga ggcctggtac
    23881 cagtacggcg ctccgcgtgt ggcgggcgta accgtgtcct ggagtttcga ataaaggagc
    23941 gctcatgcag atctcggtgg acctcagcct gtacccgctg cgcgacgact acatcccggt
    24001 gatcatcgac ttcatccatc gcgtgcaggc gcacgagggc gttgaagtcg tgcgcaacca
    24061 gatgacgacc cagctgttcg gcgattacgg atgcgtgatg gcggtgctca ccgaggagct
    24121 gcgccacagc ttcgagaccc acggcaaggc cgtgatcgtc gccaggatcg tcaacggcga
    24181 tgtgcgtgcc aatccctgag cgccggcgat gacagcgatg ctcgccggct ggctgcaact
    24241 gcacggctgg gaagccctgg ccgtgctgct cggtatcacc tacctgctgc tggcgatgcg
    24301 cgagagcctg tggtgctggc cggcggcctt cctcagcacg ctgatctacc tgttcctgtt
    24361 ctggcacgcg gagttgccga tggaatcggg gctgcagttc tactacctcg cgatggccgt
    24421 ctacggctgg tggcactggc gcggcggtac caccgaggcg gctgaacccc tgccggtacg
    24481 cacatggccg tggcgctacc acgtcgtggc catcgtgctg gtgctcgccg ccacgctggc
    24541 ctcgggagcc gcgctcacgc atgccggcct ggggcggctg ccctacctcg attcgttcac
    24601 gacctggggc gcgatcctca cgacctggat ggtggcgcag aaggtgctgg agaactggct
    24661 ctactggctg gtggtcgacg gcgtgtcact gtacctgtac ctcgaccgcg ggctgcacct
    24721 caccgcgctg ctgtttgtgg tctatcttgt gatcgtggtt ttcgggtact tccagtggcg
    24781 tcgtcattat cgtgaacaac atgcagcgtc cgatgccccg cgcgctggct gaactgttcc
    24841 gcgactggcg ccccgacagc ctcgacacca cgcccgaact ggaagccgtg ttcagctcgg
    24901 gccacagcaa ccgcagcttc ctggtgcgcc tgggccgccg gcgcatcgtc gtgcggctgc
    24961 ccgagggcaa cggggagcgc ctcggcatcg accgccacgt ggagcagcgc gtactggcgc
    25021 ttgccgaccg catcgggctc ggcgccccgg tgctctactg cgaccccgcg cgcggcatcc
    25081 tcgtcaccgc ttacctcgaa tcgaagccgc tgcgcgtcga aggcatcgcc gccgatcaca
    25141 cgatcgaccg tctcgccgcc gcgctgcacc tgctgcatcg ccaggagctc gacgtgccgg
    25201 tgctgaacat cgccgatcgc gtgcgcgcct acgcgcgcga actgcaggcg gacgatccgc
    25261 tggcatggcc gcgcgcgcgc cgctggctgg cttccgcgcg ccaggtgctg gagcagtacc
    25321 gcttcggccg ctggcacacc gcgctgtgcc acaacgacct gctggcgcag aacgtgctgg
    25381 aggtcgacgg cgcggtgcgc ttcatcgact gggaatatgc tgcgcgcggc gacgccttct
    25441 tcgatcttgc gaccatcgcg gaggactgcg gtttcggcgc gctcgaccgc agccgtctgc
    25501 tgctcgccta cggcgagatc ggcgacaccg cgatcgagcg gctgtaccgc gcgcgcgtgc
    25561 tgtaccgcct gctcggcgtg ttgtggcacc tggtgcgcta ccgcggggcg agcgcgcgca
    25621 cggtgccggc gctggtgcgc cacgagcagg cgctggatgc gctgctgcgg cagggaccag
    25681 cctaggtggg cgcgcgcgac accgcggcgc ggctgctgct gccggcactg gcgctgctgc
    25741 tgggactcgc cttcctgctg gcactgaaca cgggttcatc gggcgtgttg ttgggaccgg
    25801 cgctgcgtga ctggtacgcg ggcgaggact cggcggcatt gatcatcctg cgcgagatcc
    25861 gcctgccgcg cgcgctggcc gcgctcgcgg tcggcgccac gctgggcgtg gccggcgcgg
    25921 cgctgcaggg catgctgcgc aatccgctcg cggagccggg cctcgtgggg gcctcgaact
    25981 gcgcctcgct cggcgcggtg ctggtgctct atttcggcct tgcaccgatg cattcgctgc
    26041 tgctgccggc cgccggcatc ctgggcgccg tggcggcact cgcgctgctg cgcctgctgg
    26101 tcggggacta cgcgcgcgtc gcgacgctga tcctcgccgg tgtggcgttg aacgccttcg
    26161 ccggcgcggc gatcgcgctc gcgctgaatc tcgcgcccag cccctacgcg atgcacgaga
    26221 tcgtctactg gctgatgggc tcggtagcga accgcagttt cgaggagctc gccttcagcc
    26281 tgccgttcat ggggctcgcg ctcgcgctgc tgctgtcgac gggccgctac ctcgaggcgc
    26341 tcagcctcgg cgaggacacg gcgcattcgc tgggtttcgg cgcacgctcg ctgccctggc
    26401 gcgtgatggg cggggtggcg atcggcgtcg gcgcggcggt ggcgatcagc ggcacgatct
    26461 ccttcgtggg cctggtgatc ccgcacctgc tgcgtccctt cgtcggccac cgccccgggc
    26521 ggctgctggt gccgagcgcg ctcggtggcg cgctgctggt gctgctcgcc gacgtgctgg
    26581 tgcagtcctt ccattccgcc agcgaactga agctcggcgt ggtcacctcg ctgctcggcg
    26641 cgccgttctt cctcgcgatc gtgctcggcg cgcggagccg catcacgtga gcacgctggc
    26701 gctcgagggc gtgagcgtgc gcctcggcgg gcgcgcggtg ctgcaggaac tctcgtgcgc
    26761 gctcgccgcc ggcgagatgc tcggcatcat cgggcccaac ggcgccggca agagcacgtt
    26821 gctgcgcgtg atggcgggcc tgctgcgccc cgacgcgggg caggtgcggc tgcaggggcg
    26881 cgaactgcag tcctggccgg ccgccgagcg cgcgcggcgc atcggctacc tgccgcaggc
    26941 cgcaccggtg cactggccgc tggacgtgcg caccgtggtg gagctcgggc gcctgccgtg
    27001 gcgtcacggc tggttcagcg tcgacgaggg tgcgcgcgac gcggtgcagg ccgcgctgcg
    27061 cgatgccgag gtcgaagagc tgcagggccg tctggtgaac gagctctcgg gcggcgagcg
    27121 cacgcgcgtc atgattgcgc gcctgctggc cggcgcgccc gggatcatcc tggccgacga
    27181 gccggtggcc gcgctggatg cctaccacca gctgcacgcg atggaagtgc tggccgagcg
    27241 cgcggcgcgc ggtaccgcgg tggccgtggt gctgcacgac ctcagtctcg cggcgcgctt
    27301 ctgccagcgc atcctgctgc tcgatcacgg cggcgtcgcc gcccacggcg cggcgcgcga
    27361 catcctgtgc cccggggtcc tcgagcccgt ctacggagtg cgtatccgcg tgctcgacga
    27421 ggacgggctg gtcgcgatca tcccgtggca gcgccgctga gcatgccgga ggggggtgtg
    27481 gatgaatctg cgcatgaaag ccgccggcgc atgaatcagc cctcgtcggc aattgccacc
    27541 gctgtcgccg tgacgctgcc cgagtgggtg cccggcgtgg tcgatgcatt tcccgcatgc
    27601 acgaacgata ccgggcgcat gcgactcgca atcacgctcg cgcgcgagaa cgtcgagcgc
    27661 gctagcgggg ggcccttcgg cgcggcgatc ttcgcgcgtg gcgcaccgcg gccgctcgcg
    27721 gtcggcgtga actgtgtcga gcgcctgcgc aacgcggtgc tgcacgcgga gatcgtggcg
    27781 ctgatgctgg ccgaggcgcg cctgggctcg tacaccttgc gcgcgccgga cgcgccggaa
    27841 tacgagctgt tcacctcctg cgaaccctgt gcgatgtgcc tcggcgcgac gctgtggagc
    27901 ggcgtgcggc gcatcgtctg cgcggccggg cgcgaggacg cgatggcgct cggcttcgag
    27961 gaggggccgg tgtttcccga gacctggcgt tacctgcaag cgcaggggat cgcgctcgag
    28021 cacggcctgc tcgccgcgga ggcggctgag gtcatgcgcc ggtatcgcga gggcaacggg
    28081 ctggtctaca acggctgatg tgggtccggc ttcagtgggt gtcgcaaaac catccgtgct
    28141 ctggatagcg gcccgacaat cgggcgtatc gcgagccatc ggcgcccgag cacccatc
//
LOCUS       DAORUQ010000023        25909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1453_length_25909_cov_5.321536, whole genome shotgun sequence.
ACCESSION   DAORUQ010000023 DAORUQ010000000
VERSION     DAORUQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 25909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25909
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1453_length_25909_cov_5.321536"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLW13_03770"
     CDS             complement(<1..1285)
                     /locus_tag="PLW13_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQY69519.1"
                     /translation="MQTAPYLFGHRKYWARRFGVAPFLPMSRAEMDALGWDSCDVIVV
                     TGDAYIDHPSFGMALIGRLLEAQGFRVGIISQPDWHSAADFRALGKPNLFFGITAGNM
                     DSMVNRYTADRRIRSDDAYTPDGAPNRRPDRAVTVYAQRAREAFPEANIVIGSIEASL
                     RRIAHYDYWSDKVRRSVLPDSKADLLLFGNAERAIVTLAHRLAAGEPIAQIRDLRGSA
                     FMTPRNWSPEADWVEVDSTEVDAPGPLIRHPDPYAMETGPTAPAEPIADPAEQVLRFM
                     PQGRRKLDRTRSVVRLPAYEAVRDDPVLYAHASRTFHVESNPGNARALVQGHGERDVW
                     INPPPLPLTTPEMDGVYDLPYQRKPHPAYGAARIPAFEMIRFSVNIMRGCFGGCTFCS
                     ITEHEGRIIQSRSEDSILREIEDVRDKVQGFTGTIS"
     gene            complement(1363..2328)
                     /gene="motB"
                     /locus_tag="PLW13_03775"
     CDS             complement(1363..2328)
                     /gene="motB"
                     /locus_tag="PLW13_03775"
                     /inference="COORDINATES: protein motif:HMM:NF006548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HQY69520.1"
                     /translation="MDPSTQPIIIKKKKVVAHGHHGGSWKVAFADFVTAMMAFFLLMW
                     LLAQTSPEERTAIQGYFNDPRGSIVGQGGGLDAPGVGQVGPGGANTGAIMLDNPLTQA
                     GRSEAEPALERASDAELHAEQAEREKQALDELQRALEEELGREDSAFIKLRDQIIIDQ
                     TALGLRIQLVDNDNRPMFDSGESVLQDYAREVLLALAPRLRAVANKLSINGHTDAVAY
                     RDGANYSNWELSADRANSARRALIDGGYPEDKVLTVQGMSASVPRLADQPAAPSNRRI
                     VILVLKKDIEDALKGTTLVSRTPEEILRETDPAPAAGSAPDAAGP"
     gene            complement(2331..3182)
                     /gene="motA"
                     /locus_tag="PLW13_03780"
     CDS             complement(2331..3182)
                     /gene="motA"
                     /locus_tag="PLW13_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HQY69521.1"
                     /translation="MNVVIGLLVVLGSCVAGFMLSGGELLALFQPYELLIIGGAALGA
                     MVMSNPLGVTINVMKSFATLVKPSPYKKALYLDLLALLYDIFNKMRREGLLGIEKDID
                     DPAASPIFSKHTGIAKIHGATDFIIDYLRVMTLGGVAAHDLDALFDAELETHHTESHL
                     VSGAMSKVADALPGFGIVAAVMGIVITMGHIGGPPAELGHHVGAALVGTFLGILLSYG
                     IVGPMSVYLEHISREEAKFYECIKVSMMASFSGAPPQLAVEFGRKILFHSVRPSWTEV
                     EERVKQK"
     gene            complement(3329..3757)
                     /locus_tag="PLW13_03785"
     CDS             complement(3329..3757)
                     /locus_tag="PLW13_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HQY69522.1"
                     /translation="MLVRCLYASRPSTPLTGAVLNSILDQSRKNNPGLGVTGLLCVSD
                     DVFMQVLEGGRDEVCELYNTIVRDKRHQQVRLLSYEEITERQFGNWTMGQVQIAKINP
                     ALLLKYFKRAELNPFDCSGKATMALLGELVATASILSRGD"
     gene            complement(3765..4466)
                     /locus_tag="PLW13_03790"
     CDS             complement(3765..4466)
                     /locus_tag="PLW13_03790"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HQY69523.1"
                     /translation="MKPPSEQSPAPVSSIIRARLTRANKRFHANDNIAAFLEPGELEA
                     LLGEVEGKVKGLLESLVIDTENDHNTQDTARRVAKMYLTEVFRGRYVGPPPVTEFPNA
                     ECLNELMIVGPIKVRSACSHHFCPIMGRLWIGLMPNEHSNLIGLSKYSRLAEWVMSRP
                     QIQEEAITQMAELLMDKVRPDGLAVVMEADHFCMHWRGVKDDETMMVNSVMRGSFLKD
                     HTLRSEFLSLLNLRT"
     gene            complement(4512..5837)
                     /locus_tag="PLW13_03795"
     CDS             complement(4512..5837)
                     /locus_tag="PLW13_03795"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY69524.1"
                     /translation="MKPPPPLEVSGLSHDGRGVARRDGKTVFIAGALPGEQVRCRVTH
                     ARARFDEAQLIEVLRASPERRAPRCPHFGHCGGCTLQHLSGEAQLAAHQQQLAGALER
                     IARLQPGHWLAPVASEPFAYRARARLAVAWDAGARRAALGFRRGASRVVEPLDSCPVL
                     APAAGALIAPLAALIATLAAPGHVREVWLATGEPGLALGVACAPALDATDRARLEAFA
                     AAQGAVLHLGLIAALGEEPQWTEAGPPLRYATAPDGPVLEFQPWHFTQANRGVNRALI
                     AAVLAQLAPGPDDHVLELFCGLGNFTLPLATRAARVTGVEGDAALLDWARRNARANGL
                     ANTEFVAADLALAPAGAAWARARYDLVLLDPPRSGAAALLPALARLGARRIAYVSCDA
                     ATLARDAAVLCARGAWSLSHAGVFAMFPQTAHFESLAVFDRLAARARER"
     gene            6037..7215
                     /locus_tag="PLW13_03800"
     CDS             6037..7215
                     /locus_tag="PLW13_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69525.1"
                     /translation="MNAAVNEDSRAADIARMHECIAAMVPRLEERAPRTRAERRVPDE
                     TFAELREAGFFRLFQPVRYGGLEADPREFFRAQATLAEGCMSSAWAGGIVSVHAFQLA
                     LFDDRAQQEVWSSANDLISSSYAPMGKVTVVEGGFRLSGRWGWSSGSGHCDWVFLGAI
                     VPDDGYRTFLLPASDYRIEDTWQAMGLQGTGSNDVVVEDMFVPEYRTHRQMDGFRLDN
                     PGNRVNTAPLYRIPWGQLFARTVSNPAIGASRAALRLFIAGCQNRSSNDPTKLAGDTH
                     TQEIIARAELGIAEIDALLFRALGDMVECVEAGREIPMASRVRYRYEASRIVDKAVAI
                     VGELFANAGGRSVFLGSPIQQRFLDVNTARAHVANNPVPFSRNLGAVQLGMDSTDVFV
                     "
     gene            complement(7217..7687)
                     /locus_tag="PLW13_03805"
     CDS             complement(7217..7687)
                     /locus_tag="PLW13_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69526.1"
                     /translation="MTSRTTPRIRSTSAWDQRQIDAFLGASRIPLRLACNAGQGFPLL
                     NSLWFEPREGSLWCATHASSAIVPFLQRDPRCAFEIALNDPPYCGVRGQARALLTRDG
                     AGALLQRLIGRYLGDSNPALAAWLMSRADDEYAIRLDPTWLTAWDYRPRMQPAE"
     gene            7800..8783
                     /locus_tag="PLW13_03810"
     CDS             7800..8783
                     /locus_tag="PLW13_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQY69527.1"
                     /translation="MTGFLEALRTVPRVDAAQWEQLSLPTRWAVAARASVLLMTFTSA
                     ALGGLLALREPGFDAVLWVLALVGLLAAHATNNLINDYVDSARGVDRDNYYRARYGTH
                     VLENGLLSRRAMLGYIVATAAVALAAGAAVVWLRGELSVPLMLAGAFFVLFYTWPLKY
                     LGLGEPAVLAVWGPLMTGGAYYVACGAWSWPVALLGTVYALGPTTVIFGKHIDKLPAD
                     AAKGIRTLPVILGDRQARLWVMGMIILEFLLVGALVLANEFAWTLLLVLLNVPAARKT
                     IEVLGAPKPAERPADYPDEIWPLWFAHHAFVLNRRFSLLFLAGLLSGLLLG"
     gene            complement(8802..10286)
                     /locus_tag="PLW13_03815"
     CDS             complement(8802..10286)
                     /locus_tag="PLW13_03815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69528.1"
                     /translation="MQQLTGIDTLFLTQETERTPLHISPLLIYDPSTAPGGSVRFKDI
                     LRLFESRLHLAPIFRRKLVQVPFDLDYPYWVEAEDFDLEFHVRHIALPAPGDWRQLCI
                     QVARLHARILDRSRPLWEAYVIEGLDHIEGLPKGSFALFLKMHHAAIDGVAGAEIIGA
                     IHDTEPRAVARDATRTAPWHPDAPPTQLGLLARAWLNNLRQPARLAQTVGNLIPAARR
                     VREGLRANRFHNIRERVNTRFNGRIGGHRVVNARFFPLAQVKAIKDLVPGATVNDVIV
                     TIVAGALRKYLQAKGELPPVSLVAGAPINVRTQAEEGRGGNLVSMMTLSIYTDIADPL
                     ERLHQVHDECMSSKEYSSAIGARTLTDLTQTIPPRVAALGFRAASQTMLMTGATSPIN
                     TIISNVPGPQVPLYMAGARLLCSAGCGPLLDHMGLFHAVLSYHGGISVTVTACRDMLP
                     DPDFYTECIQASFDELKQAAEALGRSRARALQGRRPRARKAAAP"
     gene            10446..10826
                     /locus_tag="PLW13_03820"
     CDS             10446..10826
                     /locus_tag="PLW13_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HQY69529.1"
                     /translation="MIDIRLNSRRAIMRVSLIDRIQAQDFSEAVAPAAERLRARHGRI
                     EFLILDVRRFHGWGAGGTFAAQIRFLRCFGRSVERVAVFGSRAWNGATPAIAALFVAA
                     EVRSFAPGQGWLMRRWMRVRKRPA"
     gene            complement(10833..11264)
                     /locus_tag="PLW13_03825"
     CDS             complement(10833..11264)
                     /locus_tag="PLW13_03825"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY69530.1"
                     /translation="MSNVNMHVERDFDHPGDRVWAILGNFADIGWCQGMERVEVRGSG
                     PGMERHIHMPGLGAPIEEVLESIDTAARTFSYSIPRGNPMPVTDYLATVRVEELGAGR
                     CRVHWSATGEAQGIGGEQAAEILSGVYGQMLGWVADALAKG"
     gene            complement(11261..12592)
                     /gene="bioA"
                     /locus_tag="PLW13_03830"
     CDS             complement(11261..12592)
                     /gene="bioA"
                     /locus_tag="PLW13_03830"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQY69531.1"
                     /translation="MTDQRDPHAIAALLAADRRHAWHPYASALNPPPVYPVASARGVR
                     LQLADGRELLDGMSSWWAAIHGYNHPVLNRAVSAQLGRMSHMMFGGLTNEPAVELCAL
                     LAAITPGELERVFLCDSGSVAVEVAIKMALQYWQARGQPGRNRLLTLAGGYHGDTFMA
                     MSVCDPVTGMHHLFRDVLPRQLFAPRPGCGFGKPARAADIAAFEALLDEHREQIAAVI
                     LEPIVQGAGGMHFYSADYLRRVRALCDAHGVLLIADEIATGFGRTGKLFACEHAGIAP
                     DILCLGKALTGGYLSLAATLTSGHVAGIVCSGEAGALMHGPTFMGNPLACAVALASTR
                     LLLSQPWQREIARLEAALRAGLAPAALLPGVREVRVLGAIGVIEMEQEVAMGPITERF
                     LAEGVWIRPFGRLVYTMPPFIMRNDEVARLTHGMIAALEKYLSHPAGEEAR"
     gene            complement(12597..13214)
                     /locus_tag="PLW13_03835"
     CDS             complement(12597..13214)
                     /locus_tag="PLW13_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HQY69532.1"
                     /translation="MISRRLLALLFLCASALFATLPARGNAACVLVLGDSISAGLGVP
                     AGAGWVDLLRPELDGCEVVNASISGETTEGGKARLPALLQQHRPRLVVLELGGNDGLR
                     GFSLALTRANLATMIDAAHIAGARVLLLGMRIPPNYGPRYAEGFHALFGELATQTGAA
                     LVPFLLEGIATDPALMQTDGVHPTGAAQPLILANVLPVLAPLVRE"
     gene            13210..13902
                     /locus_tag="PLW13_03840"
     CDS             13210..13902
                     /locus_tag="PLW13_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY69533.1"
                     /translation="MMKPLIVASNVGKEVVTAEGALGILEGISLEINPGEAVAIVGAS
                     GSGKSTLLGLLAGLDLPSSGSIRIDGEDITRLGEDARARLRARMVGFVFQSFQLLPGL
                     TAIENVMLPLEIRGDRDAAARARDYLGRVGLAQRLGHYPRQLSGGEQQRVAIARAFAC
                     TPRLLFADEPTGNLDAATGARVIDLIFALQAEQGATLVLVTHDERLAARCARRLTLDG
                     GRLRDAVAALTC"
     gene            13896..16361
                     /locus_tag="PLW13_03845"
     CDS             13896..16361
                     /locus_tag="PLW13_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQY69534.1"
                     /translation="MLRLALRLFWRDWRGGELGVLLAALVLAVGIVSTLGLFIDRLQR
                     AVELRSATFIAGDLVLQSPRPVPADWIEAARAAGLREAEVLRFSTMVFRGDAMQLVSV
                     KAVGEGYPLLGRVGIGASPEGALEERDRGPPAGEVWVDARLLPALGIALGERIGLGAT
                     ELRVGAILRKEPDGGGSLVAMGPRLMMNLADIGAAGVLQPGSRVQYAYLFAGEAARVA
                     AWRAGIEAGLLPGQRFVTVEEGQPRVARALGRAESFLMLAGSLGVVLAGIALAMAASR
                     YTERHADYVAILKTLGLTAGAIRALYAGNLLLLALAGIALGWLCAWVLQAAVFAVLGD
                     LLGMELPAAGTRALWLGAGTGALSLLGLATPPLLAMAGASPLGVLRRERTPGAPRAAV
                     YASGLAALALLLYWYSGDWRLGLLVLGCLLASAALVGSFSYLLLRRARLPGMQAQGML
                     RLAFAALQRHAALNALQTLVFALSLMLALVMVLVRGALIDDWQRELPPRTPNHFLVNI
                     APSQLERLGEFLAGRGITHAGLYPMVPGRIAAVNGAPPRVEDGEELNLDREFNLTWSD
                     TLPAENRISAGRWWGAQGGDEVSVEAGVARALGLALGDRLTVQVGAERFEVTLSSIRE
                     LDWDTMRPNFFLVFPRRVLEKQAGMWITSFYLEPDRKLVLNELVREFPTVSVLEMDAI
                     LAQLRGITAQLALAVELVLALLVIAGALVMVASVQAGLDARFRESAILRALGAGRRLV
                     LGSLIAEFALLGMFAGTLAAAGAETVAWLVQTRLMDLAFRVHPLLWLLGPLAGAVIAA
                     LLGVFTCRRVVDTPPVVVLRELA"
     gene            complement(16362..17153)
                     /locus_tag="PLW13_03850"
     CDS             complement(16362..17153)
                     /locus_tag="PLW13_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HQY69535.1"
                     /translation="MTVDGLRKLFSGLLGRRKPAEAEPQDPESRAGFEQTVIRLQQMV
                     EERSFVDVRFPGRADSVYQSLILKVDPHERYVLIDELFPAHGQYFISPGDEVEISSVR
                     RGIPVRFHSWVKSISIDEIDGLPAYRLALPDSVQAKQRRTHFRLALDADSGVRLRIRG
                     PDGERLLCTVLNLSQNGLGFSCQGNLSDSLRGSPLLRNCLLSIPGQPDIGCDLEARSF
                     EFRKQPHRHTVVGARLDNLAPASARQLEQYLVLTQRQQRRETVRR"
     gene            complement(17227..18207)
                     /locus_tag="PLW13_03855"
     CDS             complement(17227..18207)
                     /locus_tag="PLW13_03855"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQY69536.1"
                     /translation="MKKPVRVTVTGAAGQISYSLLFRIASGAMLGPDQPVILHMLEIT
                     PALDALRGVAMELEDCAFPLLAGTVCTDKPEEAFADAQYALLVGARPRGPGMERKDLL
                     EANAAIFSVQGKAINAAAARDIRILVVGNPANTNALIAQRNAPDINPRQFTAMTRLDH
                     NRAITQLANKTGNPVTAVTHMTIWGNHSSTQYPDLYNAKVNGKAAIELVDRGWYENDF
                     IPSVQQRGAAVIKARGASSAASAANAAIDHMRTWALGTAPGDWVSMGVYSDGSYGIAQ
                     GLIYSFPCVCKDGDWQIVQGAEINDFSRAKMQATEQELTEERDAVAHLLP"
     assembly_gap    18302..18401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18423..19262
                     /gene="ttcA"
                     /locus_tag="PLW13_03860"
     CDS             18423..19262
                     /gene="ttcA"
                     /locus_tag="PLW13_03860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HQY69537.1"
                     /translation="MAPADPRKSRTEFNKLQKRLRRNVGRAIEDYNMIEAGDRVMVCL
                     SGGKDSYGMLDILLNLQRTAPVPFELVAVNLDQKQPGFPEDVLPRYLESVGIEFHILE
                     RDTYSIVKSVIPEGKTTCGLCSRLRRGNLYSFAREIGATRIALGHHRDDIIETLFLNM
                     FFNGKLKAMPPKLLSDDGDNVVIRPLAYCTEEDLAEYAACRGFPIIPCNLCGSQENLQ
                     RQAVKEMLRDWERKYPGRIESIFASIANVAPSQLADTTLFDFASLRARPRVDALIRAV
                     NVG"
     gene            19442..19798
                     /gene="fliE"
                     /locus_tag="PLW13_03865"
     CDS             19442..19798
                     /gene="fliE"
                     /locus_tag="PLW13_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HQY69538.1"
                     /translation="MVERADITQVLAQMRAIQAQMQRPLHEVAPGRIEPGQLARPQSP
                     APAFGELFSQAIDSVNAVQQQANTLREGYELGQPGVSLTQVMVAAEKSSVAFEAMTQV
                     RNKLVDAYKEIMNMPV"
     gene            19831..21555
                     /gene="fliF"
                     /locus_tag="PLW13_03870"
     CDS             19831..21555
                     /gene="fliF"
                     /locus_tag="PLW13_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HQY69539.1"
                     /translation="MADTEQDAGSQLPAVAGGGAGGSAAYVAPLNPYVAGIAGLSIPR
                     QVGVIVGLAASVALAVWLVLWTQGSDMRPLYGSLENLDGAAVVGVLESNGIRYRIDEN
                     SGALLVESARFHDARLLLAEAGMPNQRAVGFEMLDKEQGIGTSQFMETARYRHSLEGE
                     LARTIGSIASVRAARVHLAIPERSAFVRDQRRPSASVLLELQPGRTLQEGQVRAIGNL
                     VASSVPDLDLADVTIVDQKGKLVSNFGDDPEAVAATRQLAYTAKLEDQLVQRVHRILD
                     PIVGGGRYKAEVTADVDFTAVEQTDEIYNPDLPALRSERRLDEQRVPGDAGAGIPGAL
                     SNQPPVAGAAPEVVTAAPAGEPAAPAAPRNVRTESTRNYEVDRSLSHTRHQVGNVRRL
                     TVAVVLDDRVRVNADSGERENLPWDDAALERVTTLVRDAVGYDPARGDSVNVVNAAFF
                     DLPATESELEAVPFWQQDWFLPVVRQVLAALLVLAVLLAVVRPVLRSLSSNVKQLRAL
                     EDRHREERVRLEATTQQRVSEGQGEALLLAAPGREYRARLSAVQSMVESDAERVAQVV
                     RKWVRDSE"
     gene            21542..22564
                     /gene="fliG"
                     /locus_tag="PLW13_03875"
     CDS             21542..22564
                     /gene="fliG"
                     /locus_tag="PLW13_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HQY69540.1"
                     /translation="MTVSEEKAGIEHLKPIEQAAILLLSVGESNAASIMRHLTPKELQ
                     KVGAQMANMSDVKSADVALVMEQVLVEAGAATGLGVGTETYIRNVLIEALGEERAATV
                     IDHILMSDKTKGLEGLRWMNPKLVANTIRGEHPQIQAVILSYLYPDQAAEVLAQFPDK
                     ARTDLLMRIATMETVNASALQELNKIVEGELTGSGLPQGHFLGGVKFAAEIMNNFESA
                     AEQQLMDHIKEADESLGNKIQDLMFVFDDLKNVDDQGIQALLREVSSDVLVLALKAAD
                     DELKKKIFGNMSKRAGELLRDDLEAKGPVRVSDVENAQREILAVARRMADAGEITLGA
                     SAEAMI"
     gene            22590..23369
                     /locus_tag="PLW13_03880"
     CDS             22590..23369
                     /locus_tag="PLW13_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HQY69541.1"
                     /translation="MSQQRRIPARNGASYAAWTLPAVTEGVVLRAESAAGRRIDAGSA
                     PAEMVRGESFQRELEENIRAGRYARGVSAAELEAIVRQAAEEGRREGYDEGYARGQEQ
                     GYEAGHTEGLAAGRKYLEEAAGRFAGLLESLHRPLLGQEQELREAVLEVSAQIARAVI
                     RAELAIQPQSVAHVIEEALAALPLGARNVRVYVSAADRALLEDFGAGEAVPALLVDAT
                     LAPGDCRVESAESLVDFRQSARLGAILEQFLGRTGAAAGGA"
     gene            23366..24775
                     /gene="fliI"
                     /locus_tag="PLW13_03885"
     CDS             23366..24775
                     /gene="fliI"
                     /locus_tag="PLW13_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HQY69542.1"
                     /translation="MSESALAQFVRAAGRRIRHDCAAVAEGTLTRVVGMTLEATGLNC
                     ALGQRCLIRAPGVPPVQAEVVGFNGERVFLMPAARVTGLRPGLRVRPVAEAAESPGGD
                     GLLGRVLDGSGEPIDGHGPLREVHYTALDGRPINPLHRAPIRRPLDVGVRSINALLTV
                     GRGQRLGIFAGSGVGKSMLLGMMTRFTEADVTVVGLVGERGREAREFIEDILGEEGMR
                     RAVVVVSPADDAPLMRMRCARLATRIAEDFRDRGRDVLLLMDSLTRYAQAQREIGLAV
                     GEPPATRGYPPSVFARIPDLVERAGNSEEGRGSITAFYTVLAEGDDLQDPVADAARAI
                     LDGHVVLSRALAEEGQYPAVDVEASISRAMPAITTPAHRQVAQEFKKLHARFQQNRDL
                     ISVGAYAAGSDEDTDRAVARMPSMRRYLQQALDERVSFDASVAQLDALFNSGTEARAA
                     PPATRRILRPAAEAQGSAP"
     gene            24772..25224
                     /locus_tag="PLW13_03890"
     CDS             24772..25224
                     /locus_tag="PLW13_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HQY69543.1"
                     /translation="MKRSQRLQTLVQLVQLAERGARERLAQANRELLRREQQQQQLES
                     YEQEYATRWLESGRQGIAGAQLAQLGAFRASLAGSLAIQERAVGTAREQSVLSAAQWR
                     AVRNRQRMFEDLAARARADEEREAARRLQKLMDDLPRRIDPLDAEPGT"
     gene            25286..>25909
                     /locus_tag="PLW13_03895"
     CDS             25286..>25909
                     /locus_tag="PLW13_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69544.1"
                     /translation="MPPAVLIPAVLSLPVAGDVRAPSPAGPARMAANGADPDAAQEFG
                     RALDEAMQNPGKASVPRSSAPAEREHDAPDGVPVAVAPVIAVPTLAPAPAAAAEATVG
                     SLPESGPGAASGAAGAHMAPPPQAPVGKTLPIVDQALPRTAPAAPVPAAPMLTPPLPA
                     QPTAAVASTTPQASAATPSALMPSAAGAALPATDGRATRELAPGLLEA"
BASE COUNT         3697 a         8631 c         9512 g         3969 t
ORIGIN      
        1 cggagatggt gccggtaaag ccctgcacct tgtcgcgcac gtcctcgatc tcgcgcagga
       61 tggagtcctc ggagcggctc tggatgatgc gcccctcgtg ctcggtgatc gagcagaagg
      121 tgcagccgcc gaagcagccg cgcatgatgt tgaccgagaa gcggatcatc tcgaaggcgg
      181 ggatgcgcgc cgcgccgtag gccgggtgcg gcttgcgctg gtagggcagg tcgtagacgc
      241 cgtccatctc gggagtcgtg agcgggagcg gcggcgggtt gatccacacg tcgcgctcgc
      301 cgtggccctg caccagcgcg cgcgcgttgc cggggttcga ttccacgtgg aaggtgcgcg
      361 aggcgtgggc gtagagcacc gggtcgtcgc gcacggcctc gtaggcaggc aggcgcacca
      421 ccgagcgcgt gcggtcgagc ttgcgccgtc cctgtggcat gaagcgcagc acctgttcgg
      481 ccgggtctgc gatcggctcc gcgggtgcgg tggggccggt ttccatcgcg taggggtcgg
      541 ggtgacggat cagcgggccg ggcgcatcca cttcggtcga atcgacctcg acccagtcgg
      601 cctccgggct ccagttgcgc ggcgtcatga acgcgctgcc gcgcaggtcg cggatctgtg
      661 cgatcggctc gccggcggcg aggcggtgcg ccagcgtcac gatcgcgcgc tcggcgttgc
      721 cgaacagcag caggtcggcc ttggaatcgg gcagcaccga gcggcgcacc ttgtcggacc
      781 agtagtcgta atgcgcgatg cgcctcaggc tggcctcgat cgagccgatc acgatgttgg
      841 cctcggggaa ggcctcgcgg gcgcgttgcg cgtagaccgt cacggcgcgg tcagggcggc
      901 ggttcggcgc cccgtccggc gtgtaggcgt catccgagcg gatgcggcgg tcggcggtgt
      961 agcggttgac catcgaatcc atgttgccgg cggtgatgcc gaagaacagg ttcggcttgc
     1021 cgagggcgcg gaaatccgcc gccgagtgcc agtccggctg gctgatgatg ccgacgcgaa
     1081 agccctgggc ctcgagcagg cgcccgatca gcgccatgcc gaagctggga tgatcgatat
     1141 aggcatcgcc ggtgaccacg atgacgtcgc aggaatccca gccgagcgcg tccatctcgg
     1201 cgcgcgacat cggcaggaag ggcgccacgc caaagcgcct ggcccagtac ttgcggtgac
     1261 cgaagagata gggtgcggtt tgcatgccgg cattgtaacg ccgccacgga cccgcccggg
     1321 taccggaatc gggcaataag ccccctggtc gcccgcgcgc gatcagggcc cggctgcgtc
     1381 cggcgcgctg cccgcggccg gcgccgggtc ggtttcgcgc aggatttcct caggcgtgcg
     1441 cgagaccagc gtggtcccct tcaacgcgtc ctcgatgtcc ttcttcagga ccaggatcac
     1501 gatgcgccgg ttgctcggcg cggcgggctg gtcggccagg cgcggcaccg aggcgctcat
     1561 gccctgcacg gtgagcacct tgtcctcggg gtagccgccg tcgatcaggg cgcggcgcgc
     1621 actgttggcg cggtccgcgg agagctccca gttgctgtag ttcgccccgt cgcggtaggc
     1681 gacggcgtcg gtgtggccgt tgatactgag cttgttggcc accgcccgca ggcgcggggc
     1741 cagcgccagc agcacctcgc gcgcgtagtc ctgcagcacg ctctcgccgc tgtcgaacat
     1801 cgggcggtta tcgttgtcga cgagctggat gcgcagtccc agcgcggtct ggtcgatgat
     1861 gatctggtcg cgcagcttga tgaacgcgga gtcttccctg ccgagctcct cctccagcgc
     1921 gcgctgcagc tcgtccagcg cctgcttctc gcgctcggcc tgctcggcgt gcagctcggc
     1981 atccgaggcg cgctcgagcg ctggctcggc ctcgctgcgc ccggcctgtg tcagcgggtt
     2041 gtcgagcatg atggcgccgg tgttggcccc gcccggcccg acctgcccga cgccgggcgc
     2101 gtcgagtccg ccgccctggc cgacgatcga gccgcgcggg tcgttgaagt agccctggat
     2161 ggcggtgcgc tcctccggcg aggtctgcgc cagcagccac atcagcagga agaaggccat
     2221 catcgcggtc acgaagtccg cgaaggcgac cttccacgag ccaccgtgat ggccgtgcgc
     2281 gacgaccttc ttctttttga tgatgatcgg ctgggtcgag ggatccatgc tcatttctgc
     2341 ttgacgcgtt cctcgacctc ggtccagctc gggcgtaccg aatggaacag gatcttgcgc
     2401 ccgaactcga ccgccagctg cggcggggcg ccggagaagc tggccatcat cgacaccttg
     2461 atgcactcgt agaacttcgc ttcctcgcgc gagatgtgct cgaggtagac cgacatcggg
     2521 ccgacgatgc cgtaggagag caggatgccg aggaaggtgc ccaccagtgc cgcgccgacg
     2581 tgatgcccga gctccgcggg cggtccgccg atatgcccca tcgtgatcac gatgcccatc
     2641 accgcggcca cgatcccgaa gccggggagg gcgtcggcaa ccttgctcat cgcgcccgag
     2701 accaggtgcg attcggtgtg gtgggtctcg agctcggcgt cgaacagcgc atcgaggtca
     2761 tgcgccgcca cgccgcccag cgtcatcacg cgcaggtaat cgatgatgaa atccgtggcg
     2821 ccgtggatct tcgcgatgcc ggtgtgcttg ctgaagatcg ggcttgccgc agggtcgtcg
     2881 atgtccttct cgataccgag caggccttcg cggcgcatct tgttgaagat gtcgtagagc
     2941 agggcgagca ggtcgaggta gagggccttc ttgtacggcg agggcttcac cagagtggcg
     3001 aaggacttca tcacgttgat ggtcacgccg agcggattcg acatcaccat ggcgccgaga
     3061 gccgctccgc cgatgatcag caactcgtag ggctggaaca gcgcgaggag ctcgccgccg
     3121 gagagcatga atcccgcgac gcaggacccc agcacgacca gcaggccgat gacgacattc
     3181 atgtggcgat ggctccttcg agggcttggc aagcggtggt cggcaggcga ccgcgcgctc
     3241 gggcgcggcg cttcccaagg gtagaccgcg accggaatat ttccagcctc gagggcaggt
     3301 cctcaggagc gacccgaaac aggaaccctc agtcgccgcg gctgaggatc gatgcggtcg
     3361 cgaccagctc gccgagcagg gccatggtcg cctttcccga gcaatcgaag ggattgagct
     3421 cggcgcgctt gaagtatttc aggagcagcg ccgggttgat cttggcgatc tgcacctggc
     3481 ccatggtcca gttgccgaac tggcgctcgg tgatttcctc gtaactcagc agccgcacct
     3541 gctggtggcg cttgtcgcgc acgatcgtgt tgtagagctc gcagacctcg tcgcgcccgc
     3601 cctcgagcac ctgcatgaag acgtcgtcgg acacgcacag caggccggtg acgccgagac
     3661 cggggttgtt cttgcgcgac tgatcgagga tggagttcag gacggctccg gtgagtggcg
     3721 tggaaggtcg gctggcgtaa aggcaacgta cgagcatgtt ggtctcacgt gcggaggttg
     3781 aggagcgaca ggaattcgct gcgcagcgtg tgatccttga ggaaggatcc gcgcatgacg
     3841 ctgttcacca tcattgtctc gtcatccttg acaccacgcc agtgcatgca gaagtggtcc
     3901 gcttccatga ccaccgccag cccgtccggg cggaccttgt ccatgagcag ctccgccatc
     3961 tgcgtgatcg cctcctcctg gatctgcggc cgcgacatga cccattccgc gaggcgcgaa
     4021 tacttcgaca agccgatcag gttcgagtgc tcgttgggca tcaggccgat ccacagccgt
     4081 cccatgatgg ggcagaaatg atggctgcag gcgctgcgga ccttgatggg accgacgatc
     4141 atcagttcgt tgaggcattc ggcgttcgga aactcggtga ccggcggcgg tcccacgtag
     4201 cgcccgcgga acacttcggt gagatacatc ttcgccacgc gccgcgccgt gtcctgggtg
     4261 ttgtgatcgt tctcggtgtc gatcaccagg ctctcgagga ggcccttgac cttgccctcg
     4321 acctctccca gcagggcctc cagctcgccc ggctccagga atgccgcgat gttgtcgttg
     4381 gcatggaacc gcttgttcgc gcgcgtgaga cgcgcacgga tgatcgacga gaccggtgcc
     4441 ggggactgtt cactcggggg cttcacgtgc tcttccatac gttcggagtc tggctttgag
     4501 tttgcaccag tctagcgctc ccgggcccgc gcggccagac ggtcgaagac cgccagcgat
     4561 tcgaagtgcg cggtctgcgg aaacatggcg aaaacgccgg cgtgcgagag cgaccaggcc
     4621 ccgcgggcgc agagcacggc ggcatcacgg gccagcgttg ccgcgtcgca ggagacgtag
     4681 gcgatgcgcc gcgcccccag cctcgcgagc gccggcagca gcgccgcggc gccgctgcgc
     4741 ggcgggtcca gcagcaccag gtcgtagcgc gcgcgggccc acgcggcccc ggccggtgcg
     4801 agcgcgagat ccgccgccac gaactcggtg ttggcgaggc cgttggcgcg cgcattgcgc
     4861 cgcgcccagt ctagcagcgc ggcatcgccc tcgacgccgg tcacccgcgc cgcgcgcgtg
     4921 gcgagcggca gcgtgaaatt gccgagcccg cagaacagct cgagcacgtg gtcgtccggg
     4981 ccgggcgcca gctgcgccag gaccgcggcg atcagcgcgc ggttcacgcc acggttggcc
     5041 tgcgtgaaat gccagggctg gaactccagc accggcccgt ccggggccgt ggcgtaacgc
     5101 agcggcgggc cggcctcggt ccattgcggc tcctcgccga gcgcggcgat gagcccgagg
     5161 tgcagcaccg cgccctgcgc ggcagcgaag gcctccagcc gggcgcggtc ggtagcatcg
     5221 agcgcgggcg cgcaggccac gcccagcgcg agcccgggct cgccggtggc gagccacact
     5281 tcgcgcacgt gtccgggtgc ggccagggtc gcgatcagcg cagccagcgg cgcaatcagc
     5341 gcgccggccg cgggcgcgag gacggggcag ctgtcgagcg gttccaccac gcgcgaggcg
     5401 ccgcggcgaa agccgagcgc ggcgcggcgc gccccggcgt cccaggcaac ggcgagccgg
     5461 gcccgtgccc ggtaggcaaa cggctcgctc gcgaccggcg ccagccagtg gccgggctgc
     5521 agcctggcga tgcgttcgag cgcgccggcg agttgctgct ggtgcgcggc gagttgcgcc
     5581 tcgcccgaga ggtgctgcag cgtgcagccg ccacagtgtc cgaagtgcgg gcagcgaggc
     5641 gcgcgccgct cgggcgaggc gcgcagcacc tcgatcagct gcgcctcgtc gaagcgcgcc
     5701 ctggcgtggg tcacgcggca gcgcacctgt tcgcccggca gtgcgccggc gatgaacacg
     5761 gtcttgccgt cgcgccgcgc cacgccgcgg ccgtcgtggc tgaggcctga tacctcgagc
     5821 ggtggtggtg gtttcatgcg tgttggcccc tgacccggcg cgcatgttag cccctggggc
     5881 ggcgcgcgcc cacgcgattg cgcaataatg cgccgttgcg cgtccacttg ttcgctacac
     5941 tgggcaccgc tgctgcatgt cgtctcgcgg tgaaccgccg gcacgggcgc ggcgtacagg
     6001 gaaatcgaaa aaccacgacc ggatcgagga gaaagcatga acgccgccgt caacgaggat
     6061 tcgcgtgccg ccgatatcgc gcgcatgcac gagtgcatcg ccgcgatggt gccgcggctg
     6121 gaggagcgcg cgccacgcac gcgggccgag cggcgcgttc ccgacgagac cttcgcggaa
     6181 ctgcgcgagg cgggtttctt ccgcctgttc cagcccgtgc gctacggcgg cctggaggcg
     6241 gacccgcgcg agttcttccg cgcccaggcg acgctcgccg aaggctgcat gtcctcggcc
     6301 tgggccgggg gcatcgtgtc ggtgcacgcg ttccagctgg cgctgttcga cgatcgcgcg
     6361 cagcaggaag tgtggtcgag cgcgaacgac ctgatctcct cgtcctacgc gccgatgggc
     6421 aaggtcacgg tggtggaggg cggctttcgc ctctccgggc gctggggctg gtcgagcggc
     6481 agcggccact gcgactgggt gttcctcggc gcgatcgtgc ccgacgacgg ctaccgcacc
     6541 ttcctgctgc cggcgagcga ttaccgcatc gaggacacct ggcaggcgat gggcctgcag
     6601 ggcacgggca gcaacgacgt cgtcgtcgag gacatgttcg tgcccgaata ccgcacgcac
     6661 cgccagatgg acggctttcg gctggacaat ccgggcaacc gcgtgaacac cgcgccgctg
     6721 tatcgcattc cgtggggcca gctgttcgcg cgcacggtat cgaaccccgc gatcggcgcc
     6781 tcgcgcgcgg cgctgcggct gttcatcgcc ggctgccaga accgttccag caacgatccg
     6841 accaagctcg ccggcgacac ccacacgcag gagatcatcg cgcgcgccga gctcggcatc
     6901 gccgagatcg acgcgctgct gttccgggcg ctgggcgaca tggtcgagtg cgtggaggcg
     6961 gggcgcgaga tcccgatggc ctcgcgggtg cgttaccgct acgaggcctc gcgcatcgtc
     7021 gacaaggcgg tggcgatcgt cggcgagctg ttcgccaacg ccggcgggcg ctcggtgttc
     7081 ctcggcagcc cgatccagca gcgcttcctc gacgtgaaca cggcgcgcgc ccacgtcgcc
     7141 aacaacccgg tgccgttctc gcgcaacctc ggcgcggtgc aactcggcat ggacagcacg
     7201 gacgtgttcg tgtgaatcac tccgcgggct gcatgcgcgg acggtagtcc caggccgtga
     7261 gccaggtcgg gtcgaggcgg atcgcgtact cgtcatcggc gcggctcatc agccaggcgg
     7321 ccagcgcggg gttgctgtcc ccgagatagc gcccgatcag gcgctgcagc aatgcgcccg
     7381 cgccgtcgcg ggtgagcagg gcgcgtgcct gcccgcgcac gccgcagtag ggcgggtcgt
     7441 tcagcgcgat ttcgaaggcg caacgcggat cgcgctgcag gaacggcacg atggccgagc
     7501 tggcatgggt ggcgcaccac aggctgccct cgcgcggctc gaaccacagc gagttcagca
     7561 gcggaaagcc ctggcccgcg ttgcatgcca ggcgcagcgg tatgcggctg gcgcccagga
     7621 acgcgtcgat ctggcgctgg tcccaggcgc tggtggaacg gatacgcggt gtggtgcggg
     7681 atgtcacgat tcgactcctg tcatgatgcg atgctgattc taggccaagc gagaatgcgc
     7741 ggcgaggcgc gggagcgtcc cgttatactg cggctcgcaa tgtttgcagg gagtttgccg
     7801 tgaccggatt tctcgaggcg ctgcgcaccg ttccacgcgt ggatgcggcg caatgggagc
     7861 aattgtcgct accgacgcgc tgggccgtgg ccgcgcgcgc ctcggtgctg ttgatgacgt
     7921 tcacgtcggc ggcactgggc ggcctgctgg cgctgcgcga accgggattc gacgccgtgc
     7981 tgtgggtgct tgcgctcgtc ggcctgctgg cggcccacgc caccaacaac ctgatcaacg
     8041 actacgtcga ctcggcgcgt ggcgtcgatc gcgacaatta ctatcgcgcg cgctacggca
     8101 cccacgtgct ggagaacggc ctgctcagcc ggcgcgcgat gctcggttac atcgtcgcga
     8161 cggcggccgt ggcgctggca gcgggtgcgg ccgtggtgtg gctgcgcggg gagctgagcg
     8221 tgccgctgat gctggccggc gcgttcttcg tgctctttta cacctggcca ctgaaatatc
     8281 tcgggctcgg cgagcccgcg gtgctggcgg tgtgggggcc gttgatgacg ggcggggcgt
     8341 attacgtcgc ctgcggcgcg tggagctggc cggtggcgct gctcggcacc gtgtatgcgc
     8401 tcggtcccac caccgtgatc ttcggcaagc acatcgacaa gcttcccgcc gacgcggcca
     8461 agggcatacg cacgctgccg gtgatcctcg gcgatcggca ggcgcggctg tgggtgatgg
     8521 ggatgatcat cctggaattc ctgctggtcg gcgcgctggt gctcgccaat gaattcgcgt
     8581 ggacactgct gctggtgctg ctgaacgtgc cggcggccag gaaaaccatc gaagtgcttg
     8641 gcgcgcccaa gcctgccgag cgtcccgccg actaccccga tgagatctgg ccgctgtggt
     8701 tcgcccacca cgcgttcgtg ctcaaccgcc gcttttccct gctctttctc gcgggcctgc
     8761 tgtcggggct gctgctgggc tgacccggca cgcgccggcg ctcatggtgc ggccgccttg
     8821 cgcgcgcgcg ggcgccgccc ctgcagcgcc ctggcgcggc tgcgcccgag cgcctcggcc
     8881 gcctgcttga gttcgtcgaa gctggcctgg atgcactcgg tgtagaaatc cgggtccggc
     8941 agcatgtcgc gacaggcggt cacggtcacc gagatgccgc cgtggtagct cagcaccgca
     9001 tggaacagcc ccatgtgatc cagcagcggt ccgcagccgg cgctgcacag caggcgcgcg
     9061 ccggccatgt acagcggcac ctgcggtccc ggcacgttcg agatgatcgt gttgatgggc
     9121 gaggtggcac cggtcatcag catggtctgg ctcgcggcgc gaaacccgag cgccgccacg
     9181 cgcggcggga tcgtctgcgt gaggtcggtc agggtgcgcg cgccgatggc actggagtac
     9241 tccttggacg acatgcattc gtcgtgcacc tggtgcaggc gctcgagcgg atcggcgatg
     9301 tcggtgtaga tggagagcgt catcatgctc accaggttgc cgccgcggcc ctcctcggcc
     9361 tgggtgcgca cgttgatggg tgcgcccgcg accagcgaga cgggcggcag ctcgcccttg
     9421 gcctgcaggt acttgcgcag cgcgccggcc acgatcgtca cgatgacgtc gttcacggtc
     9481 gccccgggca ccaggtcctt gatcgccttg acctgcgcca gcgggaagaa gcgcgcgttg
     9541 accacgcggt gtccgccgat acgcccgttg aagcgcgtgt tgacgcgctc gcggatgttg
     9601 tggaagcggt ttgcgcgcag gccctcgcgc acgcgcctgg cggccgggat caggttgccc
     9661 acggtctgcg ccaggcgtgc cggctggcgc aggttgttca gccaggcgcg cgccagcagg
     9721 ccgagctgcg tgggtggcgc atcggggtgc cacggcgccg tgcgcgtggc atcgcgcgcc
     9781 acggcgcgcg gctcggtgtc atggatggcg ccgatgatct cggcgccggc aacgccgtcg
     9841 atcgccgcgt ggtgcatctt caggaacagc gcgaaggatc ccttcggcag gccctcgata
     9901 tggtccagcc cctcgatcac gtaggcttcc cacaacggac gcgagcggtc gaggatgcgt
     9961 gcatgcaggc gcgcgacctg gatgcagagc tggcgccagt caccgggcgc gggcagcgcg
    10021 atgtggcgca cgtggaactc gaggtcgaag tcctcggcct cgacccagta cgggtagtcg
    10081 aggtcgaagg gcacctgcac cagcttgcgg cgaaagatcg gcgcgaggtg cagccggctc
    10141 tcgaacaggc gcaggatgtc cttgaagcgc acgctgccgc cgggcgccgt ggaggggtcg
    10201 tagatcagca gcggcgagat gtgcagcggc gtgcgttcgg tctcctgggt gaggaacagg
    10261 gtgtcgatgc cggtcagctg ctgcatggaa gggctccgcg gggaaatgac gcgccccgag
    10321 tatagcggtc ggcagtggct gttgtgagcg ttacggctgg cgtggataat cgaccgggag
    10381 cggctcggga ggagccgcgc cggggttgcc ggcatcacgc ggtccagaca cggaggtcta
    10441 cggccatgat cgacatcagg ctcaattcgc ggcgcgcgat catgcgcgtc agtctcatcg
    10501 accggatcca ggcgcaggat ttcagcgagg ccgtggcgcc cgcggcggaa cggctgcggg
    10561 cgcgccacgg gcgcatcgag ttcctgatcc tcgacgtgcg gcgcttccac ggctggggcg
    10621 ccgggggcac cttcgccgca cagatccgtt tcctgcgctg tttcggacgc agcgtcgagc
    10681 gcgttgcggt gttcggctcg cgggcgtgga acggcgcgac gccggcgatc gccgcgctgt
    10741 tcgtggcggc ggaggtgcgc agtttcgcgc cgggccaggg ctggctgatg cgccgctgga
    10801 tgcgcgtgcg caagcggccc gcttgagcgg actcagccct tggccagcgc gtctgcgacc
    10861 cagccgagca tctggccgta gacgccgctc aggatctccg cggcctgttc gccgccgatg
    10921 ccctgcgcct cgcccgtcgc gctccagtgc acgcggcagc gtccggcgcc gagttcctcg
    10981 acccgcaccg tggcgaggta gtcggtgacc ggcatcgggt tgccgcgcgg gatcgagtag
    11041 ctgaaggtgc gggcggcggt gtcgatggat tccagcacct cttcgatcgg tgcgcccagg
    11101 cccggcatgt ggatgtggcg ttccatgccc gggccgctgc cgcgcacctc gacgcgctcc
    11161 atgccctggc accagccgat gtcggcgaaa ttgccgagga tggcccagac gcgatcgccc
    11221 ggatgatcga aatcgcgttc cacatgcatg ttcacgttgc tcatcgcgcc tcttctcctg
    11281 cggggtgtga cagatatttt tccagcgcgg cgatcatacc gtgcgtgagg cgagccacct
    11341 catcgtttcg catgatgaac ggcggcatgg tgtagaccag tcgcccgaag ggccggatcc
    11401 acacgccctc ggcgaggaag cgctcggtga tcgggcccat cgcgacctcc tgctccatct
    11461 cgatcacgcc gatggcgccg agcacgcgca cctcgcgcac gccgggcaga agggccgccg
    11521 gcgccagccc cgcgcgcagg gccgcttcca ggcgcgcgat ctcgcgctgc cagggctgcg
    11581 agagcagcag ccgggtgctc gccagcgcca ccgcgcaggc gagcgggttg cccatgaagg
    11641 tcggaccgtg catcagcgcg ccggcctcgc cgctgcagac gatcccggcc acgtgcccgc
    11701 tcgtgagcgt cgcggccagc gagaggtagc cgccggtcag cgccttgccg aggcagagga
    11761 tgtcgggcgc gatgccggcg tgctcgcagg cgaagagctt gccggtgcgc ccgaagccgg
    11821 tggcgatctc gtcggcgatc agcagcaccc cgtgcgcgtc gcacagtgcg cgcacccggc
    11881 gcaggtagtc cgcggagtag aagtgcatgc cgcccgcgcc ctgcacgatg ggctcgagga
    11941 tcacggccgc gatctgctcg cggtgctcgt cgagcagcgc ctcgaaggcg gcgatgtccg
    12001 cggcgcgcgc gggcttgccg aaaccgcagc cggggcgcgg cgcgaacagc tgccggggca
    12061 gcacgtcgcg gaacaggtgg tgcatgccgg tgaccggatc gcataccgac atcgccatga
    12121 aggtgtcgcc gtggtagccc ccggccagtg tcagcaggcg attgcgcccg ggttgccccc
    12181 gcgcctgcca gtactgcagc gccatcttga tcgccacttc caccgcgacc gagcccgagt
    12241 cgcagaggaa aacgcgctcg agctcgcccg gcgtgatcgc cgcgagcagc gcgcacagct
    12301 cgacggccgg ctcgttggtg agcccgccga acatcatgtg cgacatgcgc ccgagctgcg
    12361 cgctgacggc gcggttcagc accgggtggt tgtagccgtg gatcgcggcc caccacgacg
    12421 acatgccgtc gagcagttcg cgcccgtcgg cgagttgcag gcgcacgccg cgcgcgctgg
    12481 cgacggggta gaccggcggc ggattcagcg ccgaggcgta gggatgccag gcgtggcggc
    12541 ggtcggcggc cagcagtgcg gcgatcgcgt gcggatcgcg ctgatccgtc acgcgctcat
    12601 tcccgtacca gaggcgcgag caccggcagc acgttggcga ggatcagcgg ctgcgccgcg
    12661 ccggtggggt ggacgccgtc ggtctgcatc agcgccgggt cggtcgcgat gccctcgagc
    12721 aggaacggga ccagcgccgc gccggtctgc gtggcgagct cgccgaacag cgcgtggaag
    12781 ccctcggcgt agcgcgggcc gtagttcggc ggaatgcgca tgccgagcag cagcacgcgg
    12841 gcgccggcga tgtgggcggc gtcgatcatc gtcgcgagat tggcgcgcgt gagcgcgagc
    12901 gaaaagccgc gcaggccgtc gttgccgccg agctcgagca ccacgagccg cgggcggtgc
    12961 tgttgcagca gcgcgggcag gcgcgccttg ccgccctcgg tcgtttcacc gctgatgctg
    13021 gcgttcacga cctcgcagcc gtcgagctcg gggcgcagca ggtcgaccca gccggcaccg
    13081 gcgggcacgc cgaggccggc gctgatcgag tcgccgagca ccagcacgca ggcggcattg
    13141 ccgcgcgcgg gaagcgtggc gaataatgcc gaggcacaca ggaacaggag cgcaagcagt
    13201 cgtctgctga tcatgaaacc actgatagtt gccagcaatg tcggaaagga agtcgtcacc
    13261 gcggagggcg cgctcggcat cttggaaggg atcagtctcg aaatcaaccc gggcgaggcg
    13321 gtcgcgatcg tcggcgcctc gggatccggc aagtcgacgc tgctcgggct gctggccgga
    13381 ctggacctgc ccagtagcgg cagcatccgc atcgacggcg aggacatcac gcgcctcggc
    13441 gaggacgcgc gcgcgcgcct gcgcgcgcgc atggtcggct tcgtgttcca gtctttccag
    13501 ctgctgccgg gcctcaccgc gatcgagaac gtgatgctgc cgctggaaat ccgcggcgat
    13561 cgcgacgcgg cggcgcgcgc gcgcgattac ctcgggcgcg tgggcctcgc gcagcgactg
    13621 gggcattacc cgcgccagct ctccggcggc gagcagcagc gcgtggcgat cgcgcgcgcc
    13681 ttcgcctgca cgccgcgcct gctgttcgcc gacgagccga ccggcaacct ggatgccgcc
    13741 accggcgcgc gcgtgatcga cctgatcttc gcgctgcagg ccgagcaggg cgcgacactg
    13801 gtgctggtca cgcacgacga gcggctcgcc gcgcgctgcg cgcggcgtct cacgctcgat
    13861 ggcgggcgcc tgcgcgacgc cgtggcggca ctcacatgct gaggctggcg ctgaggctgt
    13921 tctggcgcga ctggcgcggc ggcgagctcg gcgtgctgct cgccgcgctg gtgctcgcgg
    13981 tgggcatcgt gagcacgctc gggctgttca tcgaccgcct gcagcgcgcc gtggagttgc
    14041 gcagcgccac cttcatcgcc ggtgacctgg tgctgcagtc gccgcgcccg gttcccgccg
    14101 actggatcga ggcggcgcgc gccgccggcc tgcgcgaggc cgaggtgctg cgcttctcga
    14161 cgatggtgtt ccgcggggat gccatgcagc tggtgtcggt gaaggccgtg ggcgagggct
    14221 acccgctgct cgggcgcgtc ggcatcggcg catcgcccga gggagcgctc gaggagcgcg
    14281 atcgcgggcc tcccgccggc gaggtctggg ttgatgcgcg actgctgccc gcgctcggga
    14341 ttgcgctagg tgagcgcatc gggctcggcg cgacggagct tcgagtcggc gcgatcctga
    14401 ggaaggagcc cgatggcggc ggcagcctgg tcgcgatggg cccgcggctg atgatgaacc
    14461 ttgcggacat cggcgcggcc ggggtgctgc agccgggcag ccgcgtgcag tacgcctacc
    14521 tgtttgccgg cgaggccgcg cgcgtcgccg cctggcgtgc cggcatcgaa gccgggttgt
    14581 tgccagggca gcgcttcgtg acggtcgagg agggccagcc gcgcgtcgcg cgcgcgctcg
    14641 gtcgggccga gtccttcctg atgctggccg ggtcgctcgg cgtggtgctg gcgggcatcg
    14701 cgctggcgat ggcggcgagc cgctacaccg agcgccacgc cgattacgtc gccatcctga
    14761 agacgctggg cctgaccgct ggcgcgatcc gcgcgctcta cgccggcaac ctgctgctgc
    14821 tcgcgctcgc cgggatcgcg ctcggctggc tctgcgcctg ggtgctgcag gccgcggtgt
    14881 tcgcggtgct cggcgacctg ctcgggatgg agctgccggc ggcggggacg cgggcattgt
    14941 ggctgggcgc gggcaccggc gcgctcagcc tgctcgggct cgcgacaccg ccgctgctcg
    15001 cgatggcggg cgcttcgcca ctcggcgtgc tgcgccgcga gcgcacgccg ggggccccgc
    15061 gcgccgcggt gtacgcgagc ggcctcgcgg ccctggcact gctgctgtat tggtacagcg
    15121 gggactggcg gctcggcctg ctggtgctcg gctgcctgct ggcgagcgcc gcgctcgtgg
    15181 gctccttcag ctacctgctg ctgcgccggg cgcgcctgcc cggcatgcag gcgcagggaa
    15241 tgctgcgcct cgccttcgcc gcgctgcaac ggcatgcggc gttgaacgcg ctgcagacgc
    15301 tggtgttcgc gctcagcctg atgctcgcgc tggtcatggt gctggtgcgc ggggcattga
    15361 tcgatgactg gcagcgcgag ctgccgccgc gcacgccgaa ccatttcctg gtgaatatcg
    15421 cgccgtcgca gctcgagcgg ctcggggaat tcctggccgg gcggggcatc acgcacgccg
    15481 ggctctaccc gatggtgccc gggcgcatcg cggcggtgaa cggcgcgccg ccgcgggtgg
    15541 aggacggcga ggagctgaac ctcgatcgcg agttcaacct cacgtggtcc gacacgctgc
    15601 ccgcggagaa ccgcatcagc gccgggcgct ggtggggagc gcagggcggc gacgaggtct
    15661 cggtggaggc gggggtggcg cgcgcgctgg gactggcgct cggcgaccgg ctcacggtgc
    15721 aggtgggcgc ggagcgcttc gaggtcacgc tgagcagcat ccgcgagctc gactgggaca
    15781 ccatgcgccc gaatttcttc ctggtattcc cgcgccgggt gctggagaag caggccggca
    15841 tgtggatcac cagcttttac ctggagcccg atcgcaagct ggtgctgaac gagctggtgc
    15901 gcgagttccc cacggtgtcc gtgctggaga tggacgcgat cctggctcag ctgcgcggta
    15961 tcaccgcgca gctcgcgctg gcggtggaac tggtgctggc gctgctggtg atcgccggcg
    16021 cgctggtgat ggtcgccagc gtgcaggcgg ggctcgatgc gcgctttcgc gagagcgcga
    16081 tcctgcgcgc gctcggcgcc ggacggcggc tggtgctcgg cagcctgatc gcggaattcg
    16141 cgctgctcgg gatgttcgcc ggcaccctgg cagcggcggg cgcggaaacg gtggcgtggc
    16201 tggtgcagac gcggctgatg gacctggcgt ttcgcgtgca cccgctgctc tggctgctgg
    16261 ggccgctcgc gggtgcggtg attgccgcgt tgctcggcgt gttcacctgc cgtcgcgtgg
    16321 tggacacgcc accggtggtg gtgctgcgcg agctggcctg atcagcgtcg cacggtttcg
    16381 cggcgctgct ggcgctgcgt gagcacgagg tactgctcga gctgcctggc gctggccgga
    16441 gcaaggttgt cgaggcgcgc gccgaccacg gtgtggcggt gcggctgctt gcggaactcg
    16501 aagctgcgcg cctcgagatc gcagccgata tcgggctgcc ccgggatgct cagcaggcag
    16561 ttgcgcagca gcgggctccc gcgcaggctg tcgctcaggt tgccctggca gctgaagccg
    16621 aggccgttct gcgacaggtt cagcacggtg cagagcaggc gctcgccgtc ggggccgcgg
    16681 atgcgcaggc gcacgccgct gtcggcgtcg agcgccaggc ggaaatgggt gcggcgctgc
    16741 ttggcctgca cgctgtcggg cagcgccagg cggtaggcgg gcaggccgtc gatctcgtcg
    16801 atgctgatgg acttgaccca gctgtggaag cgcaccggta tgccgcggcg cacgctgctg
    16861 atctcgacct cgtcgccggg ggagatgaag tattgtccgt gcgcggggaa cagttcgtcg
    16921 atcagcacgt agcgctcgtg cgggtccacc ttgaggatca ggctctggta gacgctgtcg
    16981 gcgcgcccgg ggaagcgcac gtcgacgaag ctgcgctcct ccaccatctg ctgcaggcgg
    17041 atcacggtct gctcgaagcc cgcgcgcgac tcgggatcct ggggctctgc ttccgcgggc
    17101 ttgcgccttc ccagcaatcc gctgaacagc tttctcagtc cgtctacggt catgcgtttc
    17161 gccatcggtt cgagcgcggc ggggggacgg gcacggggac gcgagcggct cccgtgcccg
    17221 ggccggtcag ggcagcaggt gagcgactgc gtcgcgctcc tcggtgagct cctgttcggt
    17281 ggcctgcatt ttcgcgcgcg agaaatcgtt gatctccgcg ccctgcacga tctgccagtc
    17341 gccgtccttg cacacgcagg ggaaggagta gatcaggccc tgcgcgatgc cgtagctgcc
    17401 gtcgctgtag acgcccatgc tgacccagtc gccgggcgcg gtgcccagcg cccacgtgcg
    17461 catgtggtcg atggcggcat tggccgccga tgccgcgctg gaggcgccgc gcgccttgat
    17521 cacggcggcg ccgcgctgct gcaccgacgg gatgaagtcg ttctcgtacc agccgcggtc
    17581 gaccagctcg atggccgcct tgccgttgac cttcgcgttg tacaggtcgg ggtactgcgt
    17641 cgaggagtgg ttgccccaga tcgtcatgtg cgtgaccgcg gtgaccgggt tgccggtctt
    17701 gttcgccagt tgcgtgatgg cgcggttgtg gtcgaggcgc gtcatcgcgg tgaactggcg
    17761 cgggttgatg tccggcgcgt tgcgctgggc gatcagcgca ttggtgttgg ccgggttgcc
    17821 gaccacgagg atgcggatgt cgcgcgcggc ggcggcgttg attgccttgc cctgcaccga
    17881 gaagatcgcg gcgttggctt cgagcaggtc cttgcgctcc atgcccgggc cgcgcggacg
    17941 cgcgcccacc agcagcgcgt actgcgcgtc ggcgaaggct tcctcgggct tgtcggtgca
    18001 caccgtgccc gccagcagcg ggaaggcgca gtcctcgagc tccatcgcca cgccgcgcag
    18061 tgcgtcgagc gcgggcgtga tttccagcat gtgcaggatc accggctggt cggggccgag
    18121 catcgcgccc gaggcgatac ggaacagcag gctgtagctg atctggccgg cggcgccggt
    18181 gacggttacg cgaacgggct ttttcatctc gggggttcct gtcgactggg agggaaaacg
    18241 gcggccgggc cgcacgcgga gggcgccgat tgtagcgcct ggccccacct gagccaacac
    18301 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccagcaccc aggagatgcc
    18421 ccgtggcccc cgcggacccg cgcaagagcc gcaccgagtt caacaagctg cagaagcgcc
    18481 tgcggcgcaa tgtcggccgc gcgatcgagg actacaacat gatcgaggcg ggcgatcgcg
    18541 tgatggtctg cctctcgggg ggcaaggact cctacgggat gctcgacatc ctgctgaacc
    18601 tgcagcgcac ggcgccggtc cccttcgagc tggtcgcggt caatctcgac cagaagcagc
    18661 ccggattccc cgaggacgtg ctgccgcgct acctggaatc cgtcggcatc gagttccaca
    18721 tcctcgagcg cgacacctac agcatcgtga agtcggtgat ccccgagggc aagaccacct
    18781 gcgggctttg ctcgcgcctg cgccgcggca acctctacag cttcgcgcgc gagatcggcg
    18841 ccacccggat cgcgctcggg caccatcgcg acgacatcat cgagacgctc ttcctcaaca
    18901 tgttcttcaa cggcaagctg aaggccatgc ccccgaagct gctcagcgac gacggcgaca
    18961 acgtggtgat ccgtccgctc gcctactgca cggaggaaga tctcgccgag tacgcggcgt
    19021 gccgcggctt cccgatcatc ccctgcaacc tctgcggctc gcaggagaac ctgcagcgcc
    19081 aggcggtcaa ggagatgctg cgcgactggg aacgcaagta ccccggacgc atcgagtcga
    19141 tcttcgcctc gatcgccaat gtcgcgccct cgcagctcgc cgacaccacg ctgttcgatt
    19201 tcgcctccct gcgcgcacgc ccgcgcgtcg atgccctgat ccgcgccgtc aacgtcggct
    19261 gagcgggcgc actgcgtgtc aacgcgggtg tcgcggcgcc gcgtcggctg gcccggaaaa
    19321 tgcttgtgct gattcgaacc ttgatcccgc gctgattccg tcaatttccc gtcgcccggt
    19381 ccgatgaccg tggcatggcg ggtgcggatc gggccgggtc ggcagccggc aggagggcgc
    19441 gatggtcgag cgagcggata tcacccaggt cctggcgcag atgcgcgcca tccaggcgca
    19501 gatgcaacgc cccctgcacg aggtggcgcc cgggcgcata gagcccggtc agctcgcgcg
    19561 cccgcagtcc ccggcgccgg ccttcggcga gctgttcagc caggccatcg acagcgtcaa
    19621 cgccgtgcag cagcaggcca acacgctgcg cgagggttac gagctcggcc agccaggcgt
    19681 gagtctcacg caggtcatgg tcgcggcgga aaaatccagc gtcgccttcg aggccatgac
    19741 gcaggtgcgc aacaagctgg tcgatgcgta caaggaaatc atgaacatgc cggtctgatg
    19801 ccggtctacg cgcagggggc ctgagggggc atggcagata ccgaacagga cgccggttcg
    19861 cagttgccgg cggtggcggg gggtggcgcc ggcggcagcg ccgcgtacgt ggcgccgctc
    19921 aatccctatg tcgccggcat tgccggcctg tcgattccgc ggcaggtggg cgtgatcgtc
    19981 ggcctggcgg ccagcgtggc gctggcggtg tggctggtgc tgtggacaca gggctccgac
    20041 atgcgcccgc tctacggcag cctggagaac ctcgatggtg ccgcggtggt cggggtgctc
    20101 gagtccaacg ggatccgtta tcgcatcgac gagaacagtg gcgcgctgct ggtcgagtcc
    20161 gcgcgcttcc acgatgcgcg cctgctgctc gccgaggccg ggatgccgaa ccagcgcgcg
    20221 gtcggcttcg agatgctcga caaggagcag ggcatcggca ccagccagtt catggaaacc
    20281 gcgcgctatc gccacagcct cgaaggcgag ctcgcgcgca ccatcggcag catcgccagc
    20341 gtgcgcgccg cgcgcgtgca cctcgcgatt cccgagcggt ccgcgttcgt gcgcgaccag
    20401 cgccggccca gcgcctcggt gctgctcgag ctgcagcccg ggcgaacgct gcaggaaggg
    20461 caggtgcgcg cgatcggcaa cctcgtcgcc tccagcgtgc cggatctcga tctcgcggat
    20521 gtcaccatcg tggaccagaa gggcaagctg gtgtcgaact tcggcgacga ccccgaggcg
    20581 gtggccgcca ccaggcagct cgcctacacc gcgaagctcg aggaccagct ggtgcagcgc
    20641 gtgcaccgca tcctcgatcc catcgtcggc ggcgggcgct acaaggccga agtgacggcc
    20701 gacgtggatt tcaccgcggt cgagcagacc gacgagatct acaatcccga tcttcccgcg
    20761 ctgcgcagcg agcggcgact cgacgagcag cgcgtccccg gcgatgccgg cgcgggcatt
    20821 ccgggcgcgc tcagcaacca gccccccgtt gcgggcgccg cacccgaggt ggtgactgcc
    20881 gcgccggccg gggagcccgc ggccccggcg gcgccgcgca acgtgcgcac cgagtcgacg
    20941 cgcaattacg aggtcgaccg ctcgctcagc cacacccgcc accaggtggg caacgtgcgc
    21001 cggctcacgg tggccgtggt gctggacgat cgcgtgcgcg tgaacgccga cagcggcgag
    21061 cgcgagaacc tgccgtggga cgatgccgcg ctcgagcgcg tgacgacgct ggtgcgcgat
    21121 gccgtcggct acgatccggc gcgcggcgac agcgtcaacg tggtgaacgc ggccttcttc
    21181 gacctgccgg cgacggagtc ggagctggag gcggtaccgt tctggcagca ggactggttc
    21241 ctcccggtgg tgcgccaggt gctggcggcg ctgctggtgc tggcggtgct gctcgcggtg
    21301 gtgcggcccg tgctacgctc gctcagcagc aacgtgaagc agttgcgcgc gctcgaggac
    21361 cgccatcgcg aggagcgggt gcggcttgag gcgacgacgc agcagcgggt gagcgagggg
    21421 cagggcgagg cgctgctgct cgcggccccg ggacgcgaat accgggcgcg gttgtcggcg
    21481 gtgcagtcga tggtcgagag cgacgccgag cgcgtggcac aggtggtgcg caagtgggtg
    21541 cgtgacagtg agtgaggaaa aggccggtat cgagcacctc aagcccatcg agcaggcggc
    21601 gatcttgctg ctgtcggtcg gcgagtcgaa cgccgcgagc atcatgcgcc acctcacgcc
    21661 gaaggaattg cagaaggtcg gcgcgcagat ggcgaacatg agcgacgtga aatccgcgga
    21721 cgtggcgctg gtgatggagc aggtgctggt cgaggcgggg gcggccacgg ggctcggcgt
    21781 gggcaccgaa acctatatcc gcaacgtgct gatcgaggcg ctcggcgagg agcgtgccgc
    21841 cacggtgatc gaccacatcc tgatgagcga caagaccaag ggtctcgagg gactgcgctg
    21901 gatgaatccc aagctggtcg cgaacacgat ccgtggcgag cacccccaga tccaggccgt
    21961 gatcctgtcg tatctgtatc ccgaccaggc cgccgaggtc ctggcgcaat tcccggacaa
    22021 ggcgcgcacc gacctgctga tgcgcatcgc cacgatggag accgtgaacg cctcggcgtt
    22081 gcaggagctg aacaagatcg tcgagggcga gctcaccggt tcgggcctgc cgcagggaca
    22141 tttcctcggc ggcgtgaagt tcgccgccga gatcatgaac aacttcgaga gcgcggccga
    22201 acagcagctg atggaccaca tcaaggaggc ggacgagtcg ctgggcaaca agatccagga
    22261 cctgatgttc gttttcgacg acctcaagaa cgtcgacgac cagggcatcc aggcgctgct
    22321 gcgcgaggtg tcgtccgacg tgctggtgct ggcgctgaag gccgccgacg acgagctcaa
    22381 gaagaagatc ttcggcaaca tgtccaagcg cgccggcgag ttgctgcgcg acgatctcga
    22441 ggccaagggg ccggtgcgcg tcagcgatgt cgagaacgcg cagcgcgaga tcctcgcggt
    22501 ggcgcggcgc atggccgatg ccggtgagat cacgctcggc gccagcgccg aggcgatgat
    22561 atgaccgtgg tgcgcccgga ggcgtgccgg tgagccagca acgacgcatt cctgcccgca
    22621 acggcgcttc ctacgcggcg tggaccctgc ccgcggtcac cgagggcgtg gtgctcaggg
    22681 ccgagagcgc cgctgggcgg cgtatcgacg ccggctcggc accggccgag atggtgcgcg
    22741 gggagtcctt ccagcgcgag ctcgaggaga acatcagggc tgggcgctat gcgcgcgggg
    22801 tgagcgcggc cgagctcgag gccatcgtgc gccaggccgc ggaggaaggg cgccgcgaag
    22861 gttacgacga gggctatgcg cgcggccagg agcagggcta cgaggccggc cacaccgaag
    22921 gcctggcagc gggacgcaag tacctggagg aggcggcggg gcgcttcgcc gggctgctcg
    22981 agagcctgca ccggccgctg ctcggacagg aacaggagct gcgcgaggcc gtgctggaag
    23041 tgtcggcgca gatcgcgcgg gcggtgatcc gcgccgagct ggcgatccag ccgcagagcg
    23101 tcgcgcacgt gatcgaggag gcgctggcgg cgctgccgct cggcgcgcgc aacgtgcggg
    23161 tgtatgtcag tgccgccgac cgcgccctgc tggaggactt cggcgccggc gaggcggtgc
    23221 cggcactgct cgtcgatgca acgctggcgc ccggcgattg ccgcgtcgag tctgccgagt
    23281 cgctcgtgga ttttcgccag tcggcgcgcc tcggcgcgat tctcgaacag ttcctcggcc
    23341 gcaccggcgc ggctgccggg ggcgcatgag cgagagtgcg ctcgcgcagt tcgtgcgtgc
    23401 cgccgggcgg cggatccgcc atgactgcgc cgcggtcgcc gagggcacgc tgacccgcgt
    23461 ggtcggcatg acgctggagg caacgggatt gaactgcgcg ctcggccagc gttgcctgat
    23521 ccgtgcgccc ggcgtgcccc ccgtgcaggc cgaggtggtc ggtttcaacg gcgagcgcgt
    23581 gttcctgatg cccgcggcgc gggtcaccgg gctgcgcccg ggcctgcgcg tgcgacccgt
    23641 cgcggaagcc gccgagtcgc cgggcggcga cgggctgctc gggcgcgtgc tcgacggttc
    23701 gggcgaaccg atcgacgggc acggcccgct cagggaggtg cattacaccg cgctcgacgg
    23761 gcgtcccatc aatcccctgc accgcgcgcc cattcgccgc ccgctcgacg tgggcgtgcg
    23821 ttcgatcaac gccctgctga ccgtcgggcg tggccagcgg ctcggtatct tcgccggcag
    23881 cggcgtgggc aagagcatgc tgctcggcat gatgacgcgt ttcacggagg ccgacgtcac
    23941 ggtggtcggg ctggtcggcg agcgcggtcg cgaggcgcgc gagttcatcg aggacatcct
    24001 cggcgaggag ggcatgcgcc gcgccgtggt cgtggtctcg cccgccgacg acgcgccgct
    24061 gatgcgcatg cgctgcgcgc ggctggcgac gcgcatcgcc gaggattttc gcgatcgcgg
    24121 ccgcgacgtg ctgctgctga tggattcgct gacacgttac gcgcaggcgc agcgcgagat
    24181 cgggctcgcg gtcggcgagc cgccggcgac gcgcggctac ccgccctcgg tgttcgcgcg
    24241 cattcccgac ctggtcgagc gcgccggcaa cagcgaggag gggcgcggct cgatcacggc
    24301 cttctacacg gtgctcgccg agggcgacga cctgcaggat ccggttgccg acgccgcgcg
    24361 tgcgattctc gatggccacg tggtgctgtc tcgggcgctg gccgaggaag gacaatatcc
    24421 ggcggtcgat gtcgaggcct cgatctcgcg cgccatgccg gcgatcacga caccggcgca
    24481 ccggcaggtg gcgcaggagt tcaagaagct gcacgcgcgc ttccagcaga accgcgacct
    24541 gatcagcgtg ggcgcctacg cggccggcag cgacgaggac acggaccgcg cggtggcgcg
    24601 catgccctcg atgcgccgct acctgcagca ggcgctggac gaacgggtca gcttcgacgc
    24661 cagcgtcgcg cagctcgacg cgctgttcaa ctccggcacg gaagcgcgcg ccgcgccgcc
    24721 cgcaacgcgt cgcatcctgc ggcccgctgc cgaggcgcag ggcagcgccc cgtgaagcgt
    24781 tcccagcgcc tgcagacgct ggtgcagctg gtgcagctcg ccgagcgcgg cgcgcgcgag
    24841 cgcctggcgc aggccaaccg cgagctgctg cggcgcgagc agcagcagca acagctggaa
    24901 tcctacgaac aggaatacgc cactcgctgg ctcgaaagcg gtcgccaggg cattgccggg
    24961 gcgcaactgg cgcagctcgg cgcgttccgc gccagcctcg ccggctcgct ggcgatccag
    25021 gagcgcgcgg ttggcaccgc gcgcgagcag tccgtgctga gtgccgcgca gtggcgcgcg
    25081 gtgcgcaacc gccagcgcat gttcgaggat ctcgccgcgc gggcgcgcgc cgacgaggag
    25141 cgcgaggccg cccggcgcct gcagaagctg atggacgacc tgccgcggcg catcgatccc
    25201 ctggacgcgg agcccgggac gtaagggacg ctggcgcggc ggttgcatag gtccgtccaa
    25261 cgcgtggcgc gcccggagac cgacgatgcc cccagctgtc ctgattcctg ccgtattgtc
    25321 gttgcctgtc gcgggcgatg tgcgcgcgcc atccccggcg ggtcctgccc gcatggcggc
    25381 caacggcgcg gatcccgacg cagcgcagga gttcgggcgc gcgctcgatg aggccatgca
    25441 gaatcccggc aaggcttccg tgccgcgcag cagcgcgcct gcagagcgcg aacacgacgc
    25501 gcccgacggc gtgccggtcg ccgtggcgcc cgtcattgcc gtaccgacgc tggcgccggc
    25561 gcccgcggcc gcggccgagg cgacggtggg ttccttgccg gagtccggtc ctggcgctgc
    25621 cagcggcgcc gccggcgcac acatggcgcc gccgccgcag gcgccggtgg gcaagacctt
    25681 gccgatcgtc gaccaggcat tgccgcgtac cgcgccggcg gcgcccgtgc ccgcggctcc
    25741 catgctcaca ccgccgttgc cagcgcagcc cacggcggcg gttgcgagca cgaccccgca
    25801 agccagtgcc gccacgccat ctgcgctgat gcccagcgct gccggggcgg cgctcccggc
    25861 gacggatggc agggcgaccc gggagctggc gccggggctg ctcgaggca
//
LOCUS       DAORUQ010000024        25615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1482_length_25615_cov_6.584116, whole genome shotgun sequence.
ACCESSION   DAORUQ010000024 DAORUQ010000000
VERSION     DAORUQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 25615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25615
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_25615_cov_6.584116"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLW13_03900"
     CDS             <1..872
                     /locus_tag="PLW13_03900"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQY69545.1"
                     /translation="HGATGKGNDQVRFELGAYALMPGVRVIAPWREWDLTSRETLLSY
                     CETHNIPVEMKRGKKSPYSMDANLLHISYEGGGLEDPYWEPEEDMWRWSVAPEAAPDK
                     PTYIELGFRAGDVVSVDGQAMSPASVMIHLNQVAGANGVGRLDLVENRYVGMKSRGCY
                     ETPAGTVILKAHRAIESLTLDREVAHLKDELMPRYASLVYTGYWWSPERHMLQTAIDQ
                     SQQYVNGTVRLKLYKGSVQVVGRKSDDSLFDPHIATFEDDRGAYNQKDAEGFIRLNAL
                     RMRIAANAGRKLG"
     gene            872..2458
                     /locus_tag="PLW13_03905"
     CDS             872..2458
                     /locus_tag="PLW13_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQY69546.1"
                     /translation="MDPAALEQEVARRRTFAIISHPDAGKTTITEKLLLFGNAIQMAG
                     TIKARKSGRHATSDWMTMEQERGISVTSSVMQFPYRERVVNLLDTPGHEDFSEDTYRT
                     LTAVDSVLMVIDAAKGVEDRTIKLMEVCRLRDTPILGFINKMDRETRDPIEVLDEIES
                     VLGIKAAPVNWPIGSGKRFKGLYDLTTDTIHVYQQGQGHRIPDDVRIQGIDSPEAAAL
                     LGDLLDELKMQIELVRGASHEFDHAEYLAGRMTPVFFGTALGNFGVREMLDAFVEWAP
                     GPAAHASETRRVKAAEQPFSGFVFKIQANMDPRHRDRIAFLRVCSGAYEQGMKMRHVR
                     IGKDVRVADAVTFLAGERSATEFAVAGDIIGLHNHGTIQIGDTFSEGEALKFTGIPHF
                     APELFQRIRLKDPLKLKHLQKGLQQLSEEGSTQVFMPLRNNDLIVGAVGTLQFEVVAF
                     RLRDEYKVDCVYEPINVYTARWVECEDARKLEDFRAKAHDNLALDGGGHLAYLAPTRV
                     NLSLTEERWPDIRFRATREH"
     gene            complement(2535..3017)
                     /locus_tag="PLW13_03910"
     CDS             complement(2535..3017)
                     /locus_tag="PLW13_03910"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY69547.1"
                     /translation="MTSIYDFSCKTSKGEPKKLKDYAGKVLLIVNTASKCGLTPQFEG
                     LEALHDKYAARGLAILGFPCNQFGHQDPGSDAEILEFCQLKYDVKFPMFAKIEVNGAG
                     ADPLYKYLKKEAPGLLGSEAIKWNFTKFLVDREGNVVERFAPTDTPESIEKKIVALLA
                     "
     gene            3163..4146
                     /locus_tag="PLW13_03915"
     CDS             3163..4146
                     /locus_tag="PLW13_03915"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY69548.1"
                     /translation="MPSQAQPPRTFSTAHPEVYRFPFPAFPNGWFPLCFADEVIPGNV
                     LPLHRFGRDLVAWRDGDGAVRVADAYCPHLGAHLGHGGTVIDGELRCPFHHWRYDGSG
                     ACVFAAGARRLPPNAKLQLLPALERNRLVFAWHDAAGRAPGYEIDRVPEIESGGYREV
                     RRHVWTVRSHPQEMMENAVDITHFEAMHRWKAHAIDWIPEGPRYQMRIKVDNSGEGYQ
                     SATAANADDVVSYNVGPGFSYTRFTGRLRAISLNLMTPTEAGVVYNPQSFWVAPDIDE
                     ALAQGWAEGFLRDYADDIPVWEHKVFRATPALAEVDGPFARYRRWYAQFYA"
     gene            4213..5625
                     /locus_tag="PLW13_03920"
     CDS             4213..5625
                     /locus_tag="PLW13_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQY69549.1"
                     /translation="MLAGAYAFVDLETTGCNPVYDRITEIAIVSVRDGAVTERWQQLL
                     DPGMSIPEAIQSLTGITNAMVAGQPAFEDVAGEVQRRLEGVTFVAHNARFDHGFLRNA
                     LKRCNLTLRGPVLCTVKLSRRLFPQHARHNLDAIIQRHGLSCAARHRALGDAEATWQF
                     VAQVAASGIADLAQHIDTLTQRPSLPARLSWEALDEIPDTPGVYLFYDERDTPLYVGK
                     SVRLRSRVLAHFAADHSSHREMQMAQQTARVEWRESAGELGALLREAQLVKDLAPIYN
                     RQLRRHAALVSIRWNNEADGIPEIVGGGDIAGDGLAQLYGLFRSKAQAKKALLGLADE
                     YRLCLRLVGLEKPAPGTCFRHQVGRCGGACGGHEPLLAHQTRLATALAKLKLVTWPWR
                     GAIGIREQQSGGGLEELHVVDRWCYLGTFRSEEELREREPLARRPAFDMDTYKLLVKH
                     LMRSRHPQVVPLPSVGQASA"
     gene            complement(5776..6771)
                     /locus_tag="PLW13_03925"
     CDS             complement(5776..6771)
                     /locus_tag="PLW13_03925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY69550.1"
                     /translation="MQLSILDQSIVRPGSDARAAFAETLALAGHAERLGYERFWVSEH
                     HDSPVIAGSSPEVLLAAIGAATTTIRIGSGGVMLPHYSAYKVAENFAVLSNLYPGRVD
                     LGVGRAPGADMNAAIALAADGRPKFERFPQLLRTLTEALWNPAFRPRVGPVPAGQIPV
                     WVLGTSPDSALLAAELGLPYAVALFINPQFDPRIVEAYRSRFRPSPPQPEPRVMIAMN
                     AFCADDEAHAATMTRAADITYIRFLTQRGEIAMCSPEEAARHEFSPQEHAFVQGVSRV
                     RAVGTAQTIRARVTQLATQFHADEVMAVCNSFHFADRLRSFELLAQAFAAPATAV"
     gene            complement(6790..6866)
                     /locus_tag="PLW13_03930"
     tRNA            complement(6790..6866)
                     /locus_tag="PLW13_03930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6830..6832),aa:Met,seq:cat)
     gene            complement(6953..8044)
                     /gene="ychF"
                     /locus_tag="PLW13_03935"
     CDS             complement(6953..8044)
                     /gene="ychF"
                     /locus_tag="PLW13_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQY69551.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTRAGIAASNFPFCTIEPNTGIV
                     PVPDPRQDRIAEIVKPQRIVPTTMEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFADDNVIHVASRIDPVNDIGVINTELALADLDSAERQLQRVTRFAKGGDKDAIR
                     QKDLLEKLVPHLNEGFPARALALSDDEKATLREFNLLTAKPTMYIANVAEDGFENNPH
                     LEAVEKFAAAEGAMVVPICNKLESEIAELEEDERAEFLAELGFEEPGLNRVIRAGYGL
                     LNLQTYFTAGVQEVRAWTVRVGARAPEAAGVIHTDFQKGFIRAEVIAYADFVQYKGEQ
                     GAKDAGKWRLEGKEYVVQDGDVIHFRFNV"
     gene            complement(8047..8637)
                     /gene="pth"
                     /locus_tag="PLW13_03940"
     CDS             complement(8047..8637)
                     /gene="pth"
                     /locus_tag="PLW13_03940"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQY69552.1"
                     /translation="MSTPLALIVGLGNPGPEYHRTRHNAGADFVSALAAQCGVTLKPE
                     TRFHGLTARVQLAGQDVRLLVPTTWMNRSGQAVGPALNFFKLAPEQLLVAYDELDLPP
                     GEVRFKFDGGHGGHNGLRDIIAALGNNRAFHRLRIGIGHPGAREQVSPHVLSRPSAAD
                     RRLIDLCIEEALHVLPEAVRGNWPIALNRLNSFRAG"
     gene            complement(8690..9298)
                     /locus_tag="PLW13_03945"
     CDS             complement(8690..9298)
                     /locus_tag="PLW13_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HQY69553.1"
                     /translation="MSGEFVLNAVARGDVGKGASRRLRNDDLVPAIVYGGTKNPSNIS
                     LSHRELVKALENEAFFSHLITLKLDGGAEEVILKDLQRHPARPRILHADFQRVSRDRK
                     LHVHIPLHFINQEKCRGVKDQGGMIQHNAVEVYVSCLPQDLPEFIEVDMLSCEVGDSV
                     HLSDLKLPAGVESVELSHGPEHDLPVATVLAPKGGAAEETAS"
     gene            complement(9396..10340)
                     /locus_tag="PLW13_03950"
     CDS             complement(9396..10340)
                     /locus_tag="PLW13_03950"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQY69554.1"
                     /translation="MSEMMLFTGSANPELAQKIVQRLGLRLGCATVSRFSDGEISIEI
                     IENVRGKDVFVIQSTCAPTNDNLMELILMIDALRRSSASRITAVVPYFGYSRQDRRVR
                     SSRVPISAKVVADMMAKAGVNRVLTVDLHAEQIQGFFDVPVDNVYGSAILIDDIERQR
                     YENLMVVSPDIGGVVRARAIAKQMNDLDLAIIDKRRPRANEAQVMHIIGDVAGRTCMV
                     VDDMVDTAGTLCKAAQALKEHGAAKVIAYCTHAVLSGNAIANISASVLDELVVTDTIP
                     LSEEAKRCARIRQVSSAPLLAEAVRRVSNEESISAMFA"
     gene            complement(10472..10547)
                     /locus_tag="PLW13_03955"
     tRNA            complement(10472..10547)
                     /locus_tag="PLW13_03955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10512..10514),aa:Gln,seq:ttg)
     gene            complement(10554..11396)
                     /gene="ispE"
                     /locus_tag="PLW13_03960"
     CDS             complement(10554..11396)
                     /gene="ispE"
                     /locus_tag="PLW13_03960"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQY69555.1"
                     /translation="MSGTLTLRSPAKLNLFLHVTGRRADGYHLLQTVFQLLDLCDEIR
                     FTASAAPALVLEGAVPGVAVEDNLVMKAARALAARCAPRQGAIIRLDKRIPHGGGLGG
                     GSSDAATTLLALNRLWGCGLTSAELAGIGLALGADVPVFVHGHSAWAEGIGELLQPLD
                     LPQRWYLVIRPGCAVNTAAIFNDRELTRDTPITTMAAFLTDGHRNDCEPVVRKRYPEV
                     DRALTWLAACGEARMSGTGSCVFAPFATESAAREAASRVPADWERFVARGINRSPVHE
                     ALYD"
     gene            complement(11393..12016)
                     /gene="lolB"
                     /locus_tag="PLW13_03965"
     CDS             complement(11393..12016)
                     /gene="lolB"
                     /locus_tag="PLW13_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HQY69556.1"
                     /translation="MSCVRVARRRQRAALLALCALLALAGCAPQPRRAELPADWQAHA
                     AAVGALDEWQLQGKIGVRAASGSGSAFLTWKQQAESYRLVLSGALGMGKLVLTGDAHG
                     VAWNDSRGRTGQHADPEALVAEIWGWQLPVAALKYWIRGIPRPGHPVADAEFAGAEVT
                     RFRQSGWLVEPGAYRDVDGLALPTRLRLEAEGAILTLLINRWGPPAP"
     gene            complement(12013..13779)
                     /locus_tag="PLW13_03970"
     CDS             complement(12013..13779)
                     /locus_tag="PLW13_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY69557.1"
                     /translation="MSILPPSLRTPLTQRLARTATAALCGVLIAACAATPATAPQDAA
                     APATDTALGTPAAEPVYRPFPEDALYSLLVAEFAAHRGAFDVALQNYLQQAARTRDPG
                     VAARATRMARYMGAERATLDAALLWVELEPQNPEARFTAATELARAGRPAEAFEHMRA
                     LDAGSGQTNFAAVTASALDLPASDRQQMLEALQDIEGDSPDLLIARAVLLQSLERNDE
                     ALEAVATVLEAEPDNYQALLLEAQVYQNIGDLPKAFARVERALADDPGNKRLRLQYAR
                     LLAKTDLAKAEEQFRILVEQNPADAELRLALALVYRESGQFDRMSEELNTLLAEGNQS
                     SAAHFYLAQDAERRGDNEDAIRHYLEIRPGQMFMASITRAAELIIASHGIEALGEPLA
                     QLRARWVAQAVPLTLLESELRLEKGDLSGAQRLLDAALADTPAEPGLLYARSMVSERR
                     GDIAAMERDLRAMLRLDPDSALAMNALGYSLANLTDRHAEALALIERALALKPDDPAI
                     IDSMGWVQYRLGHLEKALTYLRQAFEKYPDHEVAAHLGEVLWVSGNREEAGEIWRKGL
                     ESKPDSRLIHDTMRRLGAIPAP"
     gene            13952..15238
                     /gene="hemA"
                     /locus_tag="PLW13_03975"
     CDS             13952..15238
                     /gene="hemA"
                     /locus_tag="PLW13_03975"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQY69558.1"
                     /translation="MSLLVVGLNHTTAPVEVRERVAFAPEQVPEALRRACGTGGVAEA
                     VILSTCNRTEFIAVTGEGAEAEERVLDWFAGSHHLARGELEGCLYRMRGPEAMRHLIQ
                     VASGLDSMVMGEPQIFGQIKSAFASARHAGTVGPGLSRAMNHVFAVAKQVRTETAIGE
                     SPVSVAFAAVKLARHIFTELGSTTALLIGAGETIELVARHLSEAGVSRLVVANRTLAR
                     AEQVAARFAGEAILLSEIPACLLRADIVISSTASQLPILGKGAVEQAMRQRRHKPIFM
                     VDIAVPRDIEPQVAELPDVYLYSVDDLEQMIDDNRRAREDEARRAGVLVDAGVAAWLR
                     DRRSLDAVGTLRDYRARAERLRDQELERVLRQLRGGADAEQLLGQLARNLTNKLLHDP
                     SVQLRRAAAEGRSELLDWSRRLLGLDETPGEGERDP"
     gene            15238..16320
                     /gene="prfA"
                     /locus_tag="PLW13_03980"
     CDS             15238..16320
                     /gene="prfA"
                     /locus_tag="PLW13_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQY69559.1"
                     /translation="MKPSLLEKLAALSDRHEELAALLGEADVIADQARFRAYSREYAE
                     IEPVVQGYARYRKVLADMASAEAMVRDTDPEMRAMAQEELAHGAALREELELELQKLL
                     LPRDPRDGSNVFLEIRAGTGGDEAAIFAGDLFRMYSRYSDRLGWQVELISERPGEHGG
                     YKEVIAVVSGKDVFSRLKFESGAHRVQRVPATESQGRIHTSACTVAILPEVADIEEIE
                     INKADLRVDTYRASGAGGQHVNRTDSAIRITHLPSGIVVECQDERSQHKNRARAMALL
                     QAKLLGQAQQEQADAQASERKLQVGSGDRSERIRTYNFPQGRVTDHRINLTLYRLPEI
                     MEGDLDCVVQPLLGEYQAEQLAALAD"
     gene            16362..17204
                     /gene="prmC"
                     /locus_tag="PLW13_03985"
     CDS             16362..17204
                     /gene="prmC"
                     /locus_tag="PLW13_03985"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQY69560.1"
                     /translation="MGELLRAARAALDAQDARIDSELLLAHVLERARSYLFAHPEVVV
                     TAPQCAAYRALIARRRDGVPLAYLVGHQEFWSLELTLDGSTLVPRPETELLVELALER
                     LAAAPARVLDLGTGSGAIALALASERPQWQVLGIDRDPRALALAVANAARLRLPNARF
                     APGDWFAGLAGERFDLVVSNPPYIRDDDPCLQDAGLRHEPRHALASGPDGLDALRRIA
                     AAAPGHLGAGAWLICEHGAEQGEAVRALMRAAGLREVATHRDLAGRERATLGRNAGAA
                     ADER"
     gene            17194..17979
                     /gene="moeB"
                     /locus_tag="PLW13_03990"
     CDS             17194..17979
                     /gene="moeB"
                     /locus_tag="PLW13_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HQY69561.1"
                     /translation="MNDEELLRYSRHILLPELDVAGQQKLLDAGALVVGLGGLGSAVA
                     LYLGASGVGRLVLADADRVELSNLQRQVIHATARLGQGKAESAAASLQALNPGVRTEV
                     VAERVDAAALDRLVPGVSVVLDCSDNSATRRAVNAACRRHRVALVSAAALGWEGQLAV
                     FDARVPGSACYECLYPQGAGEDATSCAHNGIAAPVVGTMGVLQALQALKLISGAGEVT
                     AGELLVFDGLALDFHRLRVRPRPDCPVCTRPSVGPASAGHQPG"
     gene            complement(18026..20842)
                     /locus_tag="PLW13_03995"
     CDS             complement(18026..20842)
                     /locus_tag="PLW13_03995"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQY69562.1"
                     /translation="MTLVPARHVPRVLLACDDPLESQLLRSLLRALPGSRPEVMVAEP
                     DTPGWTAAVAAADCCFISFSGTLPLAQLLARLRAAAPRLRVIGVLSAQSRFGDRAELV
                     QALRAGLSDAVVLEELSATALAALVHGASARPAADTQAPATVTPPAMPVPAVEPPRPA
                     AGATLAGSWRIALAEQRASFDEGTLQRLGYAPAEVGSGLGDWKSLIHADDIDRLVGEV
                     HGVLNGSAPPHPVTYRLRARDGSWLPVACDDIAVELDELGSPRAISGRFYQSGAADGR
                     TVAADAAPAGAALQATPVAAVAADAGEDTPGGLLAQLDTAVLRLQRDSAGAFRVAWCN
                     AAAAALEHRAPGELIGLRPSEFSPPFDGFDLEEALGRVHQTGIAEVREAMALDRGEQP
                     LWRSYRIGRLAAGDVLVEAADVSEAVGARVSRRVQDEMAQYIVRAFPLTALLVDEQGR
                     VVQGIAAAGGVLGEDAASLENRTLAELLGAQAGAECRLQIQKTLNTGRIASAVYAVDG
                     ATGKQWLECRSAALRGRPGMPQRALLIVKDDTEQVRELTEARLARDQVRAAIHRVPVP
                     LFLKDIEGRYLAINPAFGELFGVEEGMLLGKTDLEVFPDELAMELHEADRRLIAGGSS
                     SLELRTVGQGAARGEHYCFGFPVGTPGRPATGTGGILIATRDLPRPRQHDAAAAAPPE
                     APASDAEDDIAGVAASVVGRVEQVLAEAGDYAGVLRRLEQLAETTLHAQALIHEVAGQ
                     AEASSPQPPIALAPLAQNIIELERVLLPASVRLDNDITDALPPAHCDPVVFHQLLLRG
                     IRHARRGLGHDGRLGIRLRPAEAGRRACVSCREGFEGTYIELVIEDSGSRLSEQQIRG
                     LTAAPARDAAAGSALDDLAQIHALVHGQGGHLQVRQGEPAGTSLHVYLRAATPEQIED
                     RAARARSTVTRFPFIRLREPRDQN"
     gene            21036..22253
                     /gene="rhlB"
                     /locus_tag="PLW13_04000"
     CDS             21036..22253
                     /gene="rhlB"
                     /locus_tag="PLW13_04000"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HQY69563.1"
                     /translation="MQDQTDAAGEAAPRPTVTFRELGLPEPLLRAVADLGFERCTPIQ
                     QEVLPYTLLGHDCIGKAQTGTGKTAAFLLTIMADLLRNPPDEPRYIGEPRALVIAPTR
                     ELVLQIGKDAEELAAHAGLHIVTLIGGVDYDRQRQQLERKLVDILVATPGRLMDFHSQ
                     RQIHLDRIEILVIDEADRMLDMGFIPQVRRIVRQTPPKSHRQTMFFSATFTEEVVRLS
                     AEWTHNPVRVEIDAQSVASKDIDQIVYLVEADRKLDLLLNLLQQPKAASVMIFANRRD
                     QVRRLHERLERAGISCGILSGEVPQHKRVKTLEAFREGHYRVLVATDVAGRGIHVEGV
                     THVVNFTLPEDPDDYVHRIGRTGRAGASGTSISFACEDDAFLLPEIEKLLGQKLPCVH
                     PDPEMLVAPKRRP"
     gene            complement(22348..22635)
                     /locus_tag="PLW13_04005"
     CDS             complement(22348..22635)
                     /locus_tag="PLW13_04005"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HQY69564.1"
                     /translation="MQRKDKQKVVDEVWTAERIRSFLDLQPPAGMDADFHRLQRAYQS
                     MRAEDFAAFVTMFVAAGGRLDARGPQQQTLLDELERHRHGAAFAEILRAAG"
     gene            complement(22640..24112)
                     /gene="gatB"
                     /locus_tag="PLW13_04010"
     CDS             complement(22640..24112)
                     /gene="gatB"
                     /locus_tag="PLW13_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQY69565.1"
                     /translation="MNTTRGTHMEWETVIGLEVHVQLATRTKIFSGASTRFGAPPNTQ
                     ACAIDLAMPGTLPVLNAEALRMAVKFGLAIGAEIAEISVFDRKNYFYPDLPKGYQISQ
                     LDHPIVSRGAVRIDLPDGSSKTIAVTRAHLEEDAGKSLHEDYHGLTGIDLNRAGTPLL
                     EIVSEPDIRSAQEAVAYLKQIHSIIRYLGISDGNMAEGSMRCDVNISLRRPGATEMGT
                     RTEIKNVNSFRFVEKAIQVERERQAELLESGASVVQETRLYDANRNETRAMRSKEVAH
                     DYRYFPEPDLLPVRVDADFIARVRAGLPELPAAKAERFRSSHGLTAYDASVLCASREL
                     ADYFEQVVAACGDAKAASNWVTVELLGLLNRENLEIGQSKVSAAQLGALIMRILDQTI
                     SGKIAKTVFEAMWAGEGEPDAIIAARGLLQVADTGAIEQLVDAALAANMEQVERYRAT
                     EATKRVKMFGFFVGQVMQASKGKANPQQVNELLRRKLDGE"
     gene            complement(24109..25563)
                     /gene="gatA"
                     /locus_tag="PLW13_04015"
     CDS             complement(24109..25563)
                     /gene="gatA"
                     /locus_tag="PLW13_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQY69566.1"
                     /translation="MHDLSLHAIRDALARREFSSAEITAHFLARIARMNPTLNAYITV
                     DPEAALAAARRADELRARGEGGPLCGVPIAHKDIFCTSGMRTSCGSRMLDNFIPPYDA
                     TVVERLAAAGCVTLGKTNMDEFAMGSSNETSYYGPVRNPWDTERVPGGSSGGSAAAVA
                     ARLAPGATATDTGGSIRQPAALCGLTGLKPTYGRVSRHGMIAFASSLDQGGPMARSAA
                     DAALLLNHMAGFDARDSTSIDTPVPDYGAGLDADLRGLRIGLPREYFGEGLNPGTERV
                     VREGLAELERQGATLHEISLPHAPLSVPAYYVIAPAECSTNLSRYDGVRYGYRCHDPL
                     DLQDMYARSRAEAFGDEVKRRILVGTYALSAGYYDAYYRKAQKIRRLIAEDFRAAFRE
                     VDVIASPASPSPAFRFGEKSADPITMYLEDIYTIAANLAGVPALSMPGGLVDGLPAGI
                     QLIGNHLQEARLLNVAHRFQQATDWHLRQPAGIA"
BASE COUNT         3810 a         8756 c         9015 g         4034 t
ORIGIN      
        1 cccacggcgc caccggcaag ggcaacgacc aggtgcgctt cgagctcggc gcctacgcgc
       61 tgatgccggg cgtgcgcgtg atcgcgccgt ggcgcgaatg ggacctcacc tcgcgcgaga
      121 cgctgctgag ctattgcgag acgcacaaca tcccggtcga gatgaagcgc ggcaagaaat
      181 cgccctactc gatggatgcc aacctgctgc acatctccta cgagggcggc ggtctcgagg
      241 acccgtactg ggagccggag gaagacatgt ggcgctggtc ggtggcgccc gaggccgcac
      301 ccgacaagcc cacctacatc gagctcggtt tccgcgccgg tgacgtggtc agcgtcgacg
      361 gccaggccat gagcccggcc agcgtgatga tccacctgaa ccaggtagcc ggcgccaacg
      421 gtgtcggccg cctcgacctg gtcgagaacc gttacgtggg catgaagtcg cgcggctgct
      481 acgagacgcc ggccggcacc gtgatcctga aggcgcaccg tgcgatcgag tcgctgacgc
      541 tggatcgcga ggtcgcgcac ctgaaggacg agctgatgcc gcgctacgcg agcctggtct
      601 acaccggcta ctggtggtcg cccgagcgcc acatgctgca gaccgccatc gaccagtcgc
      661 agcagtacgt gaacggcacc gtgcgcctga agctctacaa gggctcggtg caggtcgtgg
      721 gccgcaagtc cgacgacagc ctgttcgacc cgcacatcgc gaccttcgag gacgatcgcg
      781 gcgcttacaa ccagaaggac gccgagggct tcatccgact caatgccctg cgcatgcgca
      841 tcgcggccaa cgccggacgc aagctcggtt gatggatccc gcggcgctcg aacaggaggt
      901 cgcgcgccgg cgcaccttcg cgatcatctc gcacccggac gcgggcaaga cgaccattac
      961 cgaaaagctg ttgctgttcg gcaacgcgat ccagatggcc ggcaccatca aggcgcgcaa
     1021 gagcggtcgt catgccacct cggactggat gacgatggag caggagcgcg gcatctccgt
     1081 gacctcctcg gtgatgcagt tcccgtaccg cgagcgcgtc gtgaacctgc tcgacacgcc
     1141 cggtcacgag gacttctccg aggacaccta ccgcacgctc accgcggtgg attcggtgct
     1201 tatggtgatc gacgccgcca agggtgtgga ggaccgcacc atcaagctga tggaagtgtg
     1261 ccgcctgcgc gacacgccga tcctcgggtt catcaacaag atggaccgcg agacgcgcga
     1321 ccccatcgag gtgctcgacg agatcgagtc ggtgctcggc atcaaggccg cgccggtcaa
     1381 ctggccgatc ggctcgggca agcgcttcaa gggcctctac gacctgacga ccgacaccat
     1441 ccacgtctac cagcagggcc agggccaccg catacccgac gatgtgcgca tccagggcat
     1501 cgacagcccc gaggcggcgg cgctgctcgg cgacctgctc gacgagctga agatgcagat
     1561 cgagctggtg cgcggggcga gccacgaatt cgatcatgcc gaatacctcg cgggcaggat
     1621 gacgccggtg ttcttcggca cggcactggg caatttcggc gtgcgcgaga tgctggatgc
     1681 cttcgtcgag tgggcgccgg gcccggcggc gcacgcgagc gagacgcgac gcgtcaaggc
     1741 cgccgagcag cccttcagcg gcttcgtgtt caagatccag gcgaacatgg acccgcgcca
     1801 ccgcgaccgc atcgccttcc tgcgcgtgtg ctccggcgcc tacgagcagg gcatgaagat
     1861 gcgccacgtg cgcatcggca aggacgtgcg cgtcgccgac gccgtgacct tcctcgccgg
     1921 cgagcgctcg gcgacggagt tcgccgtggc cggggacatc atcggcctgc acaaccacgg
     1981 cacgatccag atcggcgaca ccttcagcga gggtgaggcg ctgaagttca ccggcatccc
     2041 gcacttcgcg ccggaactct tccagcgtat ccgcctgaag gacccgctga aactcaagca
     2101 cctgcagaag gggctgcagc aactctccga ggaaggctcc acgcaggtct tcatgccgtt
     2161 gcgcaacaac gacctgatcg tgggcgccgt gggcacactg cagttcgagg tggtggcctt
     2221 ccggctgcgc gacgagtaca aggtcgactg tgtctacgag ccgatcaacg tctacacggc
     2281 gcgctgggtg gagtgcgagg acgcgcgcaa gctcgaggac ttccgtgcca aggcccacga
     2341 caacctcgcg ctggacggcg gtggccacct ggcctatctc gcgccgacgc gcgtgaacct
     2401 cagcctcacc gaggaacgct ggcccgacat ccgtttccgc gccacgcgcg agcactgaag
     2461 ccgggggtcc ggcatcagcc ggacaattgt ccggatgaat acggaactgc gccggagtcc
     2521 ggatgaatcc ggacctacgc cagcagcgcc acgatcttct tctcgatcga ctccggcgtg
     2581 tcggtgggcg cgaagcgctc gaccacgttg ccctcgcgat cgaccaggaa cttggtgaag
     2641 ttccacttga tggcttccga gccgagcaac ccgggcgcct ctttcttcag atacttgtag
     2701 agggggtcag cgccggcgcc gttgacctcg atcttggcga acatcgggaa cttcacgtcg
     2761 tacttcagct ggcagaattc gaggatctcg gcgtcgctgc ccggatcctg gtggccgaac
     2821 tggttgcagg ggaagccgag tatcgccaga cccctggcgg cgtacttgtc gtgcagcgcc
     2881 tccagcccct cgaactgcgg cgtgagcccg cacttgctgg cggtgttcac gatcagcagg
     2941 accttgccgg cgtagtcctt caatttcttc ggttcgccct tcgaggtctt gcagctgaag
     3001 tcgtatatcg atgtcatggt gttcgcgctc ccgcgtgggt ggtcggcact tcctgccccc
     3061 aaaatggacc aggcgcatgc cgggatcagc ccgcataata cgccggcaag ccgcagccgg
     3121 atcaagccgg ccgcaaggta cacgacactg gagtcgagtg acatgccatc gcaagcccag
     3181 ccgccccgca ccttcagcac ggcacacccc gaggtctacc gcttcccgtt cccggctttc
     3241 ccgaatggct ggttcccgct gtgcttcgcc gacgaggtga tccccggcaa tgtgctcccg
     3301 ctgcaccgct tcggccgcga cctggtggcc tggcgcgacg gcgacggcgc ggtgcgcgtg
     3361 gccgatgcgt actgcccgca tctcggtgcg cacctggggc atggcggcac ggtgatcgac
     3421 ggcgaactgc gttgtccgtt tcatcactgg cgctacgacg gcagcggcgc ctgcgtgttc
     3481 gcggcgggcg ccaggcggct gccgcccaat gcgaagctgc agctgttgcc cgcgctcgag
     3541 cgcaaccgcc ttgtcttcgc ctggcacgat gccgcggggc gcgcgccggg ctacgagatc
     3601 gaccgcgttc ccgagatcga gagcggcggc taccgcgagg tgcgccgcca cgtgtggacc
     3661 gtgcgctcgc acccgcagga aatgatggaa aacgcggtgg acatcacgca cttcgaggcc
     3721 atgcaccgct ggaaggccca cgcgatcgac tggatccccg agggcccgcg ctaccagatg
     3781 cgtatcaagg tcgacaacag cggcgagggc taccagtccg cgaccgccgc gaatgccgat
     3841 gacgtggtct cgtacaacgt cgggccgggt ttcagctaca cgcgcttcac gggtcggctg
     3901 cgcgcaatct cgctgaacct catgaccccg accgaggccg gcgtggtcta caacccgcaa
     3961 tcgttctggg tggcgccgga catcgacgag gcgctggcac agggctgggc cgaaggcttc
     4021 ctgcgcgact acgccgatga catcccggtg tgggagcaca aggtgtttcg cgccacgccg
     4081 gcgctggccg aggtcgacgg accgttcgcg cgctaccggc gctggtacgc gcagttctac
     4141 gcctgaggtt gccgccgctc gcggcggcgt gccagaatgc tgtacatcgg tacagtttcc
     4201 ggcggagatc gcatgctggc gggcgcatac gcattcgttg acctcgagac cacgggctgc
     4261 aacccggtct acgaccgcat caccgagatc gcgatcgtca gcgtgcgcga cggcgcggtc
     4321 accgagcgtt ggcagcagct gctcgatccg ggcatgtcga tccccgaggc gatccagtcg
     4381 ctcaccggca tcacgaacgc gatggtcgcg gggcagccgg ccttcgagga cgtggccggg
     4441 gaagtgcagc ggcgcctgga gggcgtgacc ttcgtcgcgc acaacgcgcg cttcgatcac
     4501 ggctttctgc gcaacgcgct gaagcgctgc aacctgacgc tgcgcgggcc ggtgctgtgc
     4561 accgtgaagc tctcgcgccg gctgttcccg cagcacgcgc gccacaatct cgatgccatc
     4621 atccagcgcc atggtctgag ctgtgcggcg cgccaccgcg cgctgggcga tgccgaggcg
     4681 acctggcagt tcgtggcgca ggttgcggcc tcgggcattg cggaccttgc gcagcatatc
     4741 gacacactga cgcagcgccc ctcgctgccg gcgcggctgt cgtgggaggc gctcgatgag
     4801 atccccgaca cgccgggcgt ctacctgttc tacgacgagc gcgatacgcc gctgtacgtg
     4861 ggcaagagcg tcaggctgcg ctcgcgcgtg ctggcgcatt tcgccgccga tcacagcagc
     4921 catcgcgaga tgcagatggc gcagcagacc gcgcgcgtgg agtggcgcga gtcggcgggc
     4981 gagctcggcg cgctgctgcg cgaggcgcag ctggtgaagg atctggcgcc gatctacaac
     5041 cgccagctgc gccgccatgc cgcgctggtc tcgatccgct ggaacaatga ggccgacggc
     5101 attccggaga tcgtcggcgg cggcgacatc gccggggacg gactcgcgca actctacggt
     5161 ttgttccgct cgaaggccca ggcgaagaag gcactgctcg ggctcgccga tgaataccgg
     5221 ctgtgcctgc gccttgtcgg gctggaaaag ccggcgccgg gcacgtgttt ccgccaccag
     5281 gtgggcaggt gcggcggtgc ctgcggtggg cacgagcccc tgctcgcgca ccagacgcgg
     5341 ctggcgacgg cgctggcgaa gctgaagctg gtgacctggc catggcgcgg tgccataggc
     5401 atccgcgagc agcagtccgg cggcggactc gaggaactgc acgtggtcga ccgctggtgc
     5461 tacctcggca ccttccgcag cgaggaggag ctgcgcgagc gcgagcccct cgcgcgccgc
     5521 cccgccttcg acatggacac ctacaagctg ctggtgaagc acctgatgcg cagccgccac
     5581 ccgcaggtgg tgccgctgcc gtccgtgggt caggcttcag cctgacattc ctgccggtcg
     5641 ggcctgtagg tcgggtttca acccggcaac ggttcggcaa cgccgaaacc gccgcatcgc
     5701 agcacgcatc gacccttggc cgtcggcatg aaggccgacc cacagggcga tgcacccgtc
     5761 ggcatgaatg ccgacctaca ccgcggtcgc aggtgccgcg aaggcctgtg cgagcagctc
     5821 gaaggagcgc agccggtcgg cgaagtggaa gctgttgcac accgccatca cctcgtcggc
     5881 gtggaattgt gtggcgagtt gcgtcacgcg cgcgcggatc gtctgcgccg tgcccaccgc
     5941 gcgcacgcgc gagacgccct gcacgaaggc gtgctcctgg gggctgaatt cgtggcgagc
     6001 ggcctcctcg ggcgagcaca tcgcgatctc cccgcgctgc gtgaggaagc ggatgtaggt
     6061 gatgtcggcc gcgcgcgtca tcgtcgcggc gtgcgcctcg tcgtcggcgc agaacgcgtt
     6121 catcgcgatc atcacgcgcg gttcgggttg cggcggcgag gggcggaaac gactgcggta
     6181 cgcctcgacg atgcgcgggt cgaactgcgg gttgatgaac agcgccaccg cgtagggcag
     6241 ccccagttcg gccgccagca gcgcgctgtc ggggctggtg cccagcaccc acaccgggat
     6301 ctgtccggcc ggcaccggtc ccacgcgcgg gcggaaggcc gggttccaca gcgcctcggt
     6361 cagtgtgcgc agcagctgcg ggaagcgctc gaacttcggc cgcccgtcgg cggcgagcgc
     6421 gatcgccgcg ttcatgtccg cgcccggcgc gcggcccacg ccgaggtcca cgcggcccgg
     6481 gtacaggttc gagagcaccg cgaagttttc cgccaccttg taggcgctgt agtgcggcag
     6541 catcacgccg ccggagccga tgcggatcgt cgtcgtggca gcgccgatcg ccgccagcag
     6601 cacctcgggc gaactgcccg cgatcaccgg cgagtcgtgg tgctccgaca cccagaagcg
     6661 ctcgtagccg agtcgctcgg cgtggcccgc cagcgcgagg gtttcggcaa aggccgcgcg
     6721 cgcatcgctg ccggggcgta cgatggactg gtcgaggatg gagagctgca tcggctgtcg
     6781 aagaagagat ggtagcgacg ggtgggctcg aaccaccgac cccagcatta tgaatgctgt
     6841 gctctaaccg actgagctac gtcgccacgg gccgcgaatt ttccgcactg gcccctatcg
     6901 tgtcaatggc cggcgcggtt ccgcgagcgc gggaaattac cgtttcgcgc gctcagacgt
     6961 tgaagcggaa gtggatcacg tcgccatcct gcaccacgta ttccttgcct tcgaggcgcc
     7021 acttccctgc gtccttcgcg ccctgttcgc ccttgtactg cacgaaatcg gcgtaggcga
     7081 tcacctcggc gcggatgaag cccttctgga agtcggtgtg gatcacgccg gccgcctcgg
     7141 gggccctggc gccaacgcgc acggtccagg cgcgcacttc ctgcacgccg gcggtgaagt
     7201 aggtctgcag gttcagcagg ccgtagccgg cgcggatcac gcggttcagg ccgggttcct
     7261 cgaagccgag ctcggcgagg aactcggcgc gttcgtcttc ctccagctct gcaatctcgg
     7321 actcgagctt gttgcagatc ggcaccacca tcgcgccttc cgcggcggcg aacttctcga
     7381 ccgcctcgag gtgcgggttg ttctcgaagc cgtcctcggc cacgttggcg atgtacatcg
     7441 tcggcttggc ggtgagcagg ttgaactcgc gcagcgtcgc cttctcgtca tcggagagcg
     7501 ccagcgcgcg cgccgggaac ccttcgttca ggtgcggcac cagtttctcc agcaggtcct
     7561 tctggcggat cgcgtccttg tcgccgccct tggcgaagcg ggtgacgcgc tgcagctggc
     7621 gctcggcgct gtcgagatcc gcgagcgcaa gctcggtgtt gatcaccccg atgtcgttga
     7681 ccgggtcgat gcggctcgcg acgtggatca cgttgtcgtc ggcgaagcag cgcaccacgt
     7741 gcgcgatggc gtcggtctcg cggatgttgg cgaggaactg gttgccgagg ccctcgccct
     7801 tcgaggcgcc ggccaccagg cccgcaatgt ccacgaactc catcgtggtc ggcacgatgc
     7861 gctgcggctt cacgatctcc gcgatgcggt cctgccgggg atcgggtacc ggcacgatgc
     7921 cggtgttcgg ctcgatggtg cagaacggga agttcgaggc cgcgatgccg gcgcgcgtca
     7981 gcgcgttgaa gagcgtggac ttgccgacgt tgggcagtcc gacgatgccg cagttgaatc
     8041 ccatggttat cctgccctga aactgttgag tcggttcaat gcaatgggcc agttgccgcg
     8101 cacggcttcc ggcagtacgt gcagcgcttc ctcgatgcac agatcgatca ggcgccggtc
     8161 cgccgccgag ggccgcgaga gcacgtgcgg cgagacctgc tcgcgcgcgc ccgggtggcc
     8221 gatgccgatc cgcaggcggt ggaaggcgcg gttgttgccc agcgcggcga tgatgtcgcg
     8281 caggccgttg tggccgccgt ggccgccgtc gaacttgaag cgcacttcgc ccggcggcag
     8341 gtcgagctcg tcgtaggcaa cgagcagttg ctccggcgcg agcttgaaga aattcagcgc
     8401 aggccccacg gcctgtccgc tgcgattcat ccaggtcgtc ggcaccagca gacgcacgtc
     8461 ctggccggcg agttgcacgc gagccgtgag accgtggaat cgcgtctccg gcttcagcgt
     8521 cacgccgcac tgcgcggcca gcgcgctcac gaaatcggct cccgcgttgt ggcgggtgcg
     8581 atggtattcg ggtccgggat tgcccaggcc gacgatgagg gcgagaggcg tgctcacctg
     8641 ggtcaggggg ggaacgaggc tgcccgggag ggcagcctcg cgcaccggat caggatgcgg
     8701 tttcctcggc agcgccgccc ttcggcgcca gtaccgtggc caccggcagg tcgtgctcgg
     8761 ggccatggga cagctcgacg ctctcgacgc ccgcgggcag cttgaggtcg gagaggtgca
     8821 ccgagtcgcc gacctcgcag gaaagcatgt cgacttcgat gaattcgggc agatcctgcg
     8881 gcaggcagct cacgtacact tccacggcgt tgtgctggat catgccgccc tggtccttca
     8941 cgccacggca cttctcctgg ttgatgaagt gcagggggat gtggacgtgg agcttgcggt
     9001 cgcggctcac gcgctggaag tccgcgtgca ggatgcgcgg gcgcgccggg tggcgctgca
     9061 ggtccttcag gatcacttcc tcggcgccgc catcgagctt cagcgtgatc aggtgcgaga
     9121 agaacgcctc gttctccagc gccttcacca gctcgcggtg cgagagcgag atgttgctcg
     9181 ggttcttggt gccgccgtaa acgatcgcgg gcaccaggtc gtcgttacgc aggcggcggc
     9241 tcgcaccttt ccccacgtcg ccgcgtgcta cggcattcag tacaaattcg ccagacatgg
     9301 tctgtactcc tttgatgagc ggacagcctc cctgcgaccg ggatgggtgc cgcggttctt
     9361 gaaaaggaaa ggcggttgcc tttccgctcg atgcatcagg cgaacatcgc gctgatggat
     9421 tcctcgttgc tgacccggcg cacggcctcg gccagcagcg gtgcgctgct cacctggcgg
     9481 atgcgggcgc agcgtttcgc ctcctcggac agcgggatcg tgtcggtcac gaccagttcg
     9541 tccagcacgg aggcactgat gttggctatc gcgttgcccg agagcaccgc gtgcgtgcag
     9601 taggcgatca ctttcgccgc gccgtgttcc ttgagtgcct gcgcggcctt gcacagcgtg
     9661 ccggcggtgt cgaccatgtc atcgaccacc atgcaggtac ggcccgcgac atcgccgatg
     9721 atgtgcatca cctgcgcttc gttggcccgc ggacggcgct tgtcgatgat cgcgagatca
     9781 agatcgttca tctgcttggc gatggcgcgt gcgcgcacca cgccgccgat gtcgggcgag
     9841 accaccatca ggttctcgta gcgctggcgc tcgatgtcgt cgatcaggat cgccgagccg
     9901 tagacgttgt cgaccggcac gtcgaagaag ccctggatct gctcggcgtg caggtccacc
     9961 gtgagcacgc ggttaacgcc ggccttggcc atcatgtcgg ccacgacctt ggcgctgatc
    10021 ggcacgcggc tcgagcgcac gcgccgatcc tggcgcgagt agccgaagta gggcacgacc
    10081 gccgtgatgc gcgaggccga ggaacgacgc agcgcgtcga tcatcaggat cagctccatc
    10141 aggttgtcgt tggtcggcgc gcaggtcgac tggatcacga acacgtcctt gccgcgcaca
    10201 ttctcgatga tctcgatgct gatctcgccg tcgctgaagc ggctgacggt tgcgcagccc
    10261 agcctcaggc ccagccgctg cacgatcttc tgcgccagct cggggttggc gctgccggtg
    10321 aagagcatca tctctgacac gtcgggatcc ttcgtgttgg cacagcggtt aagcaaggga
    10381 cggggttcgc ggcaggtcgc gacacgcggg gggaagctgg cggttcattg cgctcgcagg
    10441 ccccgttgcg tgatgtcccg aatgaatgaa ttggctgggg cggtaggact cgaacctacg
    10501 aatggcggga tcaaaacccg ctgccttagc cacttggcga cgccccaatt gattcagtcg
    10561 tacagtgcct cgtgcaccgg ggagcggttg atgccgcggg caacgaaccg ttcccaatca
    10621 gccggtaccc gcgaggcagc ctcgcgcgcc gcgctctcag tggcgaaggg tgcgaacacg
    10681 caactgcctg tgccgctcat ccttgcctcc ccgcacgccg cgagccatgt cagcgcgcga
    10741 tcgacttcag ggtaccgttt cctgaccacg ggctcgcagt cgttgcgatg tccgtccgtc
    10801 aaaaaggcgg ccattgtcgt gatcggcgtg tcacgtgtca attcccggtc attgaaaatc
    10861 gcggcagtat tcaccgcgca accgggacgg atcaccaggt accagcgttg gggcagatcg
    10921 agcggctgca acagctcgcc gatgccctcg gcccaggcgc tgtgcccgtg cacgaacacg
    10981 ggcacgtccg cgccgagcgc gaggccgatg ccggcgagct ccgcgctcgt gagcccgcag
    11041 ccccacagcc ggttcagcgc cagcagcgtg gtggcggcat ccgagctccc gccgcccagc
    11101 ccgccgccgt gcggtatgcg cttgtcgagc cggatgattg cgccctggcg cggcgcgcag
    11161 cgcgcggcga gcgcgcgcgc ggccttcatc accaggttgt cttccaccgc cacgcccggc
    11221 actgcgccct cgagcaccag cgcgggcgcg gcgctcgcgg tgaagcggat ctcgtcgcag
    11281 agatcgagca gctggaacac cgtctgcagc aggtggtaac cgtcggcgcg ccggccggtc
    11341 acgtgcagga acaggttgag tttcgccggc gatcgcagcg tgagcgtgcc gctcatggcg
    11401 ccggggggcc ccagcggttg atcagcagcg tgagtatcgc gccctcggct tcgaggcgca
    11461 ggcgcgtcgg cagcgcgagg ccgtccacgt cgcggtacgc gccgggttcg accagccagc
    11521 ccgactggcg aaaacgcgtg acttcggcgc cggcgaattc ggcgtccgcg acggggtggc
    11581 cgggccgggg aatgccgcgg atccagtact tcagggcagc cacgggcagc tgccagcccc
    11641 agatctcggc gaccagcgcc tcggggtcgg cgtgctggcc ggtgcgcccg cgcgagtcgt
    11701 tccaggcgac gccgtgcgcg tcgcccgtga gcaccagctt gcccatgccc agcgcgccgc
    11761 tcagcaccag gcggtagctc tcggcctgct gcttccacgt gaggaaggcg ctgccggagc
    11821 cgctcgccgc gcgcacgccg atcttgccct gcaactgcca ctcgtcgagc gcgccgaccg
    11881 ccgcggcgtg cgcctgccag tctgccggca attccgcgcg ccgcggttgt ggcgcgcaac
    11941 cggccagcgc gagcagcgcg cacagcgcga gcagcgccgc ccgctgccgg cgccgggcga
    12001 cgcggacgca gctcatggcg cgggaatggc gccgaggcgg cgcatggtgt cgtggatcag
    12061 gcggctgtcg ggcttgctct ccagcccctt gcgccagatc tcgccggcct cttcgcggtt
    12121 gccgctcacc cacagcacct cgcccaggtg cgcggcgacc tcgtggtccg ggtacttttc
    12181 gaaggcctgg cgcaggtaag tgagtgcttt ttccaggtgg ccgaggcggt attgcaccca
    12241 gcccatgctg tcgatgatgg cgggatcgtc aggcttcagc gccagcgccc tctcgatcag
    12301 cgccagcgcc tcggcgtggc ggtcggtcag gttggcgagc gagtagccga gcgcgttcat
    12361 ggccagcgcg ctgtcggggt cgaggcgcaa catcgcgcgc aggtcgcgct ccatcgccgc
    12421 gatgtcgccg cgtcgctcgc tgaccatgga gcgcgcatac agcaggccgg gctccgcggg
    12481 ggtgtcggcc agtgccgcgt cgagcaatcg ctgcgcgccg ctcaggtcgc ccttttcgag
    12541 gcgcagctcc gactccagca gcgtcagcgg cactgcctgc gcgacccagc gtgcgcgcag
    12601 ttgcgccagc ggttcgccga gcgcctcgat gccgtggctg gcgatgatca gctcggcggc
    12661 gcgcgtgatc gaggccatga acatctggcc cgggcggatt tcgaggtaat gccggatcgc
    12721 gtcctcgttg tcgccacggc gttcggcgtc ctgcgcgaga tagaaatgcg ccgcgctcga
    12781 ctggttgccc tcggcgagca gcgtgttcag ttcctcgctc atgcggtcga actggccgga
    12841 ctcgcggtag accagcgcca gcgcgaggcg cagctcggcg tcggcggggt tctgctcgac
    12901 caggatgcga aactgttcct cggcctttgc gaggtcggtc ttcgccagca gtcgcgcgta
    12961 ctgcaggcgc agccgcttgt tgccgggatc gtccgccagc gcgcgctcga cgcgggcaaa
    13021 ggctttgggc aggtcgccga tgttctggta gacctgtgcc tcgagcagca gcgcctggta
    13081 gttgtcgggc tccgcctcca gcaccgtggc cacggcctcg agtgcctcgt cgttgcgctc
    13141 caggctctgc agcagcaccg ctctggcgat cagcaggtcg gggctgtcac cctcgatgtc
    13201 ttgcagtgcc tccagcatct gctggcgatc gctcgcgggc aggtccagag cactggcggt
    13261 gaccgcggca aagttggtct ggccgctgcc ggcgtcgagc gcgcgcatgt gctcgaatgc
    13321 ctcggcgggc cggccggcgc gcgccagttc ggtggctgcc gtgaagcggg cctccggatt
    13381 ctgcggttcc agttcgaccc acagcagcgc ggcatcgagc gtggcgcgct cggcgcccat
    13441 gtagcgcgcc atgcgcgtgg cgcgtgccgc cacgccggga tcgcgcgtgc gcgccgcctg
    13501 ctgcaggtaa ttctgcagcg ccacgtcgaa cgcgccgcgg tgcgccgcga actcggccac
    13561 cagcagcgaa tacagcgcgt cttcggggaa gggccggtac accggctccg cggcgggcgt
    13621 gccgagcgcg gtatccgtcg ctggcgcagc ggcatcctgt ggcgccgtgg cgggcgtggc
    13681 cgcacaggca gcgatcaaca cgccgcagag tgcggccgtt gcggtgcggg cgaggcgctg
    13741 cgtcagcggg gtgcgtaacg acgggggcaa tatgctcata ggttgcgggt cggaccgcgc
    13801 gcggggcggt ggcggcagcg ccgggagcgc gggattctgc acaatttgcc tgtaaattga
    13861 tacacttccg ccgcttttcc aatccccatc ccgccccgtt ggagtgctgt tcccggcctg
    13921 cggcgccccg cgggggagta gtttttcatc aatgagcctg ctggtcgtcg gtctcaacca
    13981 caccaccgcg cccgtcgagg tgcgcgagcg cgtggcattc gctcccgagc aggtgccgga
    14041 ggcgttgcgt cgtgcctgcg gcacgggcgg cgttgccgag gcggtgattc tctcgacctg
    14101 taaccgtacc gagttcatcg cggtcacggg cgagggcgcc gaggccgagg agcgggtgct
    14161 cgactggttc gccggttcgc accacctggc gcggggcgaa ctcgagggct gcctttaccg
    14221 catgcgcggc cccgaggcca tgcgccacct catccaggtc gccagcggac tcgattcgat
    14281 ggtgatgggc gagccgcaga tattcggcca gatcaagtcc gccttcgcct cggcccgaca
    14341 cgccggcacg gtcggcccgg gcctttcccg cgcgatgaac cacgtcttcg cggtggcgaa
    14401 gcaggtgcgc accgagaccg cgatcggcga gagcccggtg tccgtcgcct tcgcggcggt
    14461 gaagctcgcg cggcacatat tcaccgagct cggctcgacc acggcgctgc tgatcggcgc
    14521 cggcgaaacc atcgagctgg tcgcgcgcca cctctccgag gccggcgtgt cgcggctcgt
    14581 cgtcgccaac cgcacgctgg cgcgtgccga gcaggtggcc gcacgcttcg ccggcgaggc
    14641 gatcctgctg tcggagatac cggcctgcct gctgcgtgcc gacatcgtga tcagctcgac
    14701 ggcgagccag ttgccgatac tcggcaaggg tgcggtggag caggccatgc gccagcgccg
    14761 gcacaagccg atcttcatgg tcgacatcgc ggtgccgcgc gacatcgaac cgcaggtcgc
    14821 ggagctcccc gacgtctatc tctacagcgt cgatgacctg gaacagatga tcgacgacaa
    14881 ccgtcgtgcg cgcgaggacg aggccaggcg cgccggggtg ctcgtggatg ccggcgtcgc
    14941 tgcctggctg cgcgatcgtc gcagcctgga cgccgtgggg acgctgcgtg attaccgcgc
    15001 gcgcgccgaa cggctgcgcg accaggagct cgagcgcgtg ctcaggcagc tgcgtggcgg
    15061 cgccgacgcc gagcagttgc tcggccagct cgcgcgcaac ctcaccaaca agctgctgca
    15121 cgatccgagc gtgcagttgc gccgggctgc ggccgaaggg cgctccgagc tgctcgactg
    15181 gagccgccgg ctgctcggcc tcgatgaaac gccgggagaa ggcgagcgcg acccatgatc
    15241 aagccgtccc tgctggaaaa actcgccgcg ctgagcgatc gccacgagga gctcgcggcg
    15301 ctgctgggcg aggccgacgt gatcgcggac caggcgcgct ttcgcgccta cagccgcgag
    15361 tacgccgaga tcgagcccgt ggtgcagggc tacgcgcgtt atcgcaaggt gctggccgac
    15421 atggcctcgg ccgaggcgat ggtgcgcgac acggatcccg agatgcgcgc gatggcgcag
    15481 gaggagctcg cgcacggcgc cgcgttgcgc gaggaactgg agctcgagct gcagaagctg
    15541 ctgctgccgc gcgatccgcg cgatggcagc aacgtgttcc tcgagatccg cgcgggtacc
    15601 ggcggtgacg aggcggcgat cttcgccggc gacctgttcc gcatgtattc gcgctattcc
    15661 gatcgcctcg gctggcaggt cgagctgatc agcgagcgtc ccggcgagca cggcggctac
    15721 aaggaagtga tagcggtagt ctcgggcaag gacgtgttct cgcgcctcaa gttcgagtcc
    15781 ggcgcgcacc gcgtgcagcg cgtgccagcc accgaatcgc agggccgcat ccacacctcg
    15841 gcctgcaccg tcgcgatcct gcccgaggtt gcggacatcg aggagatcga gatcaacaag
    15901 gcggacctgc gcgtcgacac ctatcgcgcc tcgggcgcgg gcgggcagca cgtgaaccgc
    15961 accgactccg ccatccggat cacgcacctg ccgagcggca tcgtggtcga gtgccaggac
    16021 gagcgctccc agcacaagaa ccgcgcccgc gccatggcgc tgctgcaggc gaagctgctc
    16081 ggccaggcgc agcaggagca ggccgacgcg caggccagcg agcgcaagct gcaggtcggc
    16141 agcggcgatc gctccgagcg catccgcacc tacaacttcc cgcagggacg cgtgaccgat
    16201 caccgcatca acctcacgct gtaccggctc cccgagatca tggaaggcga cctcgactgc
    16261 gtggtgcagc cgctgctcgg cgagtaccag gccgagcagc tcgcggcgct ggcggactga
    16321 gcgtgagcca cggcggcgcg gggctggagc gtgcgcggac cgtgggagag ttgctgcgtg
    16381 ccgctcgcgc ggcgctcgat gcgcaggacg cgcgcatcga cagcgagctg ctgctggcgc
    16441 acgtgctgga gcgcgcgcgc agttacctct tcgcccatcc ggaggtcgtc gtgacggcgc
    16501 cgcagtgcgc ggcctatcgc gcgctgatcg cgcggcggcg cgacggcgtg ccgctggcct
    16561 atctcgtcgg gcaccaggaa ttctggtcgc tcgagctgac cctggacggc agcacgctgg
    16621 tgccgcggcc cgaaaccgaa ctgctggtcg agctcgcgct ggagcggctc gccgcggcgc
    16681 ccgcgcgcgt gctcgatctg ggcaccggca gcggcgcgat cgcgctggcg ctggcgagcg
    16741 aacgcccgca atggcaggtg ctgggcatcg accgcgaccc gcgcgcgctc gccctcgccg
    16801 tcgccaacgc cgcgcggctt cgcctgccga atgcgcgctt cgcgcccggg gactggtttg
    16861 ccgggctggc cggcgagcgc ttcgacctcg tggtgtccaa cccgccgtac atccgcgacg
    16921 acgatccctg cctgcaggat gccgggttgc gccatgagcc gcgccatgcg ctggcctccg
    16981 gccccgacgg cctggatgcg ctgcggcgca tcgcggccgc cgcgcccggg cacctcggcg
    17041 ccggtgcctg gctgatctgc gagcacggcg cggagcaggg cgaggcggtg cgcgcgctga
    17101 tgcgcgccgc cgggctgcgc gaggttgcca cgcaccgcga cctcgcgggc cgcgagcgcg
    17161 cgacgctggg gcgcaacgcc ggagccgctg ccgatgaacg atgaggaact gctgcgctac
    17221 agccggcaca tcctgctgcc cgagctcgac gtggccgggc agcagaagct gctggatgcg
    17281 ggcgcgctcg tggtcggcct cggcgggctg ggctcggccg tggcgctcta tctcggcgcc
    17341 agcggcgtgg ggcggctggt gcttgccgac gccgaccgcg tcgagctgtc gaacctgcag
    17401 cgccaggtga tccacgcgac ggctcgcctg gggcagggca aggcggagtc ggcggccgcc
    17461 agcctgcagg cgctcaatcc cggcgtgcgc accgaagtgg tggcggagcg tgtcgatgcc
    17521 gcggcgctcg atcgtctcgt gcccggcgtc tcggtggtgc tcgactgcag cgacaacagc
    17581 gccacgcgac gcgcggtcaa tgccgcctgc cggcgccacc gggtagcgct ggtcagcgcg
    17641 gccgcgctcg gctgggaagg gcagctcgcg gtattcgacg cgcgcgtccc gggctcggcc
    17701 tgctacgagt gcctgtatcc gcaaggcgcc ggcgaggacg cgacttcgtg cgcgcacaac
    17761 ggcatagcgg cgccggtggt cggcaccatg ggcgtgctgc aggcactgca ggcgctgaag
    17821 ctgatcagcg gcgcgggcga ggtcacggcc ggggagctgc tggtcttcga cgggctggcg
    17881 ctggattttc accgcctgcg cgtccggccg cggccggact gccccgtgtg cacgcgtccg
    17941 tccgtgggtc ccgcttcagc cggacatcag cccggataaa tccggaccca cggagatacc
    18001 cggctgaagc cggacccacg ggagatcagt tctggtcgcg cggctcgcgc aggcggatga
    18061 acgggaagcg cgtcacggtg gagcgcgccc tggccgcgcg gtcctcgatc tgctccggcg
    18121 tcgccgcgcg cagatagacg tgcaggctgg tgccggcggg ctcgccctgg cgtacctgca
    18181 ggtgaccgcc ctggccgtgc accagcgcgt ggatttgcgc gaggtcgtcg agcgcgctgc
    18241 cggcagccgc gtcacgcgcc ggggccgcgg tgagtccgcg gatctgctgt tcgctcagcc
    18301 gcgaaccgct gtcctcgatc accagctcga tgtaggtgcc ctcgaaacct tcgcggcacg
    18361 agacgcaggc gcgccggccg gcctcggccg ggcgcaggcg gatgccgagt cgcccgtcgt
    18421 gcccgagccc gcgacgggcg tgacggatgc cgcgcagcag cagctggtgg aacacgaccg
    18481 ggtcgcagtg cgccgggggc agggcgtcgg tgatgtcgtt gtccaggcgc accgaggccg
    18541 gcagcaggac gcgttcgagc tcgatgatgt tctgcgccag cggggccagc gcgatcggcg
    18601 gctgcggcga gctcgcctcg gcctgccccg caacctcgtg gatcagcgcc tgcgcgtgca
    18661 gcgtggtctc ggccagctgc tccagccggc gcaggacccc ggcgtagtcg cccgcctctg
    18721 caagcacctg ctcgacgcgc ccgaccacgg aggccgccac acccgcgatg tcatcctcgg
    18781 cgtcgctggc gggggcctcg ggcggcgccg ccgcggcggc atcgtgctgg cgcggccgcg
    18841 gcagatcgcg tgtggcgatc aggatgccgc cggtgccggt cgccggccgc cccggcgtac
    18901 cgaccgggaa tccgaagcag tagtgttcgc cgcgcgccgc gccctggccg acggtgcgca
    18961 gctccagcga ggaactgccg ccggcgatca accggcgatc ggcctcgtgc agttccatcg
    19021 cgagctcgtc ggggaagacc tcgagatcgg tcttgccgag cagcatgcct tcctcgacgc
    19081 cgaacagttc gccgaaggcg ggattgatcg ctaggtaacg gccctcgatg tccttcagga
    19141 acagcggcac cggcacgcgg tggatcgcgg cgcgcacctg gtcgcgcgcc aggcgcgcct
    19201 ccgtgagctc gcgcacctgc tcggtgtcgt ccttcacgat cagcagcgcg cgttgcggca
    19261 tcccgggtcg gccgcgcagc gccgccgagc ggcattcgag ccattgcttg cccgtggcgc
    19321 cgtccacggc atacaccgcg ctggcaatgc gaccggtatt gagggttttc tggatctgca
    19381 ggcggcattc ggcgccggct tgcgcgccga gcagttcggc gagcgtgcgg ttctcgaggc
    19441 tcgcggcgtc ctcaccgagc acgccgcccg cggccgcgat gccctgcacc acgcgtcctt
    19501 gctcgtccac gagcagcgcg gtgagcggga acgcgcgcac gatgtactgc gccatctcgt
    19561 cctgcacgcg gcgcgacacg cgcgcgccga ccgcctcgct gacatccgcc gcctcgacca
    19621 gcacatcccc cgcggccagc cgcccgatgc gatagctgcg ccagagcggc tgctcgccgc
    19681 ggtccagtgc catcgcctcg cgtacctcgg cgatgccggt ctggtgcacg cgccccagcg
    19741 cctcctcgag gtcgaaaccg tcgaacggcg ggctgaactc ggatgggcgc agtccgatga
    19801 gttccccggg cgcgcggtgc tcgagggcgg cggcggccgc gttgcaccat gccacccgga
    19861 atgcgccggc gctgtcgcgc tgcaggcgca gcacggcagt gtcgagctgc gcgagcaggc
    19921 cgccaggggt atcctccccg gcatcggctg cgacggccgc gaccggtgtg gcttggagcg
    19981 cggcgccggc gggagcggca tccgccgcga ccgtccggcc gtccgcggcg ccgctctggt
    20041 agaagcgacc gctgatagcg cgcggcgagc cgagctcgtc gagttccacg gcgatgtcgt
    20101 cgcaagccac gggcagccag ctgccgtcgc gcgcgcgtag ccggtaggtc acgggatgcg
    20161 gcggtgccga gccgttcagc acgccgtgca cctcgcccac gaggcggtcg atgtcgtcgg
    20221 cgtggatcag ggatttccag tccccgaggc cgctgccgac ttccgccggt gcatagccca
    20281 gccgttgcag cgtgccctca tcgaagctcg cccgctgctc ggccagcgcg atgcgccagc
    20341 tgccggcgag ggtggcgccg gcggccggcc gcggcggctc gaccgccggc accggcatgg
    20401 cggggggcgt tacggtcgcg ggcgcttgcg tatcggccgc cgggcgtgcg ctcgcgccgt
    20461 gcaccagcgc ggccagtgcc gttgccgaca gctcttccag cacgaccgcg tcggacaacc
    20521 cggcacgcag cgcctgcacc agctccgcgc ggtccccgaa gcgcgactgt gcgctcagca
    20581 ccccgatcac ccgcaggcgc ggcgcggcgg cgcgcaggcg cgccagcagt tgcgccagcg
    20641 gcagggtacc ggagaaggaa atgaagcagc agtcggcggc ggccaccgcg gccgtccacc
    20701 cgggggtgtc gggctcggcg accatgacct cgggacggga tccggggagc gcgcgcagca
    20761 agctgcgcag caactgcgat tccagcggat catcacaggc gagcagtacg cgcggcacgt
    20821 ggcgtgcggg aacgagcgtc atgtgtcgtg cagccttgca gtctcccgca ggtgatcgtc
    20881 gctgcccggg tgcgtgccgg gctgcacggg attctattgc catgcggcgc aggccgccag
    20941 cggtaataag gaggatcgcg ccggacctgc aattgcgggt gccgcggcca gccgttaagc
    21001 tcccgggctc gctcgattgc caatggacac gccgaatgca ggaccagacc gatgccgccg
    21061 gggaggcagc gcctcgcccc accgtgacgt tccgcgagct cggactcccc gagccgctgc
    21121 tgcgtgccgt ggccgatctg ggtttcgagc gctgcacgcc gatccagcag gaggtactgc
    21181 cctacacgct gctcggtcac gactgcatcg gcaaggcgca gaccggcacc ggcaagacgg
    21241 cggccttcct gttgaccatc atggccgacc tgctgcgcaa tccgccggac gagccgcgct
    21301 acataggcga gccgcgtgcg ctggtgatcg cgcccacgcg cgagctggta ctgcagatcg
    21361 gcaaggatgc cgaggaactg gcggcccatg ccggcctgca tatcgtgacg ctgatcggcg
    21421 gcgtcgatta cgaccgccag cgccagcagc tcgagcgcaa gctggtcgac atcctggttg
    21481 ccacgcccgg gcgcctgatg gattttcaca gccagcgcca gatccacctc gaccgcatcg
    21541 agattctggt gatcgacgaa gccgaccgca tgctcgacat gggcttcatc ccccaggtgc
    21601 ggcgcatcgt gcgccagacg ccgccgaagt cgcaccgcca gacgatgttc ttcagcgcca
    21661 cgttcaccga ggaggtcgtg cgcctgtcgg ccgaatggac ccacaacccg gtgcgcgtcg
    21721 agatcgatgc ccagagcgtg gcctcgaaag acatcgacca gatcgtttac ctggtcgagg
    21781 ccgatcgcaa gctcgacctg ctgctgaacc tgctgcagca gcccaaggcc gccagcgtga
    21841 tgatcttcgc caaccgtcgc gaccaggtgc gacgcctgca cgagcgattg gaacgcgcgg
    21901 gtatcagctg cggcatcctc tcgggcgaag tgccgcagca caagcgcgtc aagacgctgg
    21961 aagccttccg cgaggggcac taccgcgtgc tggtggccac cgacgtggcc ggccgcggca
    22021 tccacgtcga gggcgtcacg cacgtggtga acttcacgct gcccgaggat ccggacgact
    22081 acgtgcaccg catcggccgc accggtcgtg cgggcgccag cggcacctct atcagtttcg
    22141 cctgcgagga cgatgccttc ctgctgccgg agatcgagaa actgctcggg cagaaactgc
    22201 cctgcgtgca tcccgatccc gagatgctgg tcgcgcccaa gcgccgcccg taggtcgggc
    22261 ttcagcccga caaggattcg gcgcagccga atccgccgca ttcccgcagg gcacccgtcg
    22321 tcggcataaa tgccacccac acggggttca ccctgcggcg cgcaggatct ccgcgaaggc
    22381 cgcgccgtgg cggtgccgct ccagttcgtc cagcagcgtc tgctgttgcg ggccgcgcgc
    22441 gtcgaggcgc ccgcccgcgg ccacgaacat cgtgacgaaa gccgcgaaat cctcggcgcg
    22501 catgctctgg taggcgcgct gcaggcggtg gaagtcggcg tccatgcccg ccggcggctg
    22561 caggtcgaga aagctgcgga tgcgttccgc ggtccagacc tcgtcgacga ctttctgctt
    22621 gtccttgcgc tgcatcagct cattccccgt ccagtttgcg gcgcagcagc tcgttgacct
    22681 gctgcgggtt ggccttgccc ttgctcgcct gcatcacctg gccgacgaag aaaccgaaca
    22741 ttttcacccg cttggtggcc tcggtggcgc ggtagcgctc gacctgttcc atgtttgccg
    22801 ccagcgccgc gtccacgagc tgctcgatcg cgccggtgtc ggcgacctgg agcagcccgc
    22861 gtgccgcgat gatcgcgtcc ggctctccct cgccggccca catcgcctcg aacaccgtct
    22921 tggcgatctt gcccgagatg gtctggtcca ggatgcgcat gatcagtgcg ccgagctgcg
    22981 cggccgagac cttgctctgg ccgatctcga ggttctcgcg gttcagcagc ccgagcagct
    23041 ccaccgtgac ccagttcgag gcggccttgg cgtcgccgca ggccgccacc acctgctcga
    23101 agtagtcggc gagttcgcgg ctggcgcaga gcacgctggc gtcgtaggcg gtcagtccgt
    23161 ggctcgagcg gaagcgctcg gccttcgcgg ccggcagctc gggaagcccg gcgcgcacgc
    23221 gcgcgatgaa gtcggcgtcg acgcgcaccg gcagcaggtc gggctcgggg aagtagcggt
    23281 agtcgtgcgc gacttccttg gagcgcatcg cgcgcgtctc gttgcggttg gcgtcgtaga
    23341 ggcgggtttc ctgcaccacg ctggcgccgg actcgagcag ctcggcctgg cgctcgcgct
    23401 cgacctggat cgccttctcc acgaagcgga acgagttgac gttcttgatc tcggtgcgcg
    23461 tgcccatttc agtcgcaccg gggcggcgca gcgagatgtt cacgtcgcag cgcatcgagc
    23521 cctcggccat gttgccgtcg gaaatgccga ggtagcggat gatcgagtgg atctgcttca
    23581 ggtaggccac cgcctcctgg gcgctgcgga tatcgggctc cgagacgatc tcgagcaacg
    23641 gcgtgccggc gcggttcagg tcgatgccgg tcaggccgtg gtagtcctcg tgcagcgact
    23701 tgccggcgtc ctcctcgagg tgcgcgcgcg tgaccgcgat ggtcttcgac gagccgtccg
    23761 gcaggtcgat gcgcaccgcg ccgcggctca cgatcgggtg gtccagctgg ctgatctggt
    23821 agcctttcgg caggtcggga tagaagtagt tcttgcggtc gaacaccgat atttccgcga
    23881 tctcggcgcc gatcgccagc ccgaatttca ccgccatgcg cagcgcctcg gcattgagca
    23941 ccggcagcgt gccgggcatc gcgaggtcga tggcgcaggc ctgcgtgttc ggcggcgcgc
    24001 caaagcgcgt cgatgccccg gagaagatct tcgtgcgcgt ggcgagctgg acgtgcacct
    24061 ccagcccgat caccgtttcc cattccatgt gcgttccccg ggtcgtgttc aggcgatgcc
    24121 cgccggctgg cgcaggtgcc agtcggtggc ttgctgaaag cggtgcgcga cgttgagcag
    24181 gcgcgcttcc tgcaggtggt tgccgatcag ctggatgccc gccggcaggc cgtcgaccag
    24241 tccgcccggc atcgacagcg ccggcacgcc ggccaggttg gcggcgatcg tgtagatgtc
    24301 ctcgaggtac atcgtgatcg ggtccgcgct cttctcgccg aagcggaatg cgggcgaggg
    24361 cgaggcggga ctcgcgatca catcgacctc gcgaaaggcg gcgcggaagt cctcggcgat
    24421 caggcgacgg atcttctgtg ccttgcggta gtaggcatcg tagtagcccg ccgacagggc
    24481 gtaggtgccg accaggatgc gccgcttgac ctcgtcgccg aaggcctcgg cgcggctgcg
    24541 cgcgtacatg tcctgcaggt cgagcgggtc atggcagcgg tagccatagc gcacgccgtc
    24601 gtagcgcgag aggttggtcg agcattcggc cggtgcgatc acgtaatacg ccggcaccga
    24661 cagcggcgcg tgcggcaggc tgatctcgtg caacgtcgcg ccctggcgtt cgagctcggc
    24721 cagcccctcg cgcaccacgc gctcggtccc cgggttcagc ccctcgccga agtattcccg
    24781 cggcaggccg atgcgcaacc cgcgcaggtc cgcgtccagc cccgcgccat agtcgggcac
    24841 cggggtatca atgctggtgg agtcacgcgc gtcgaaaccg gccatgtggt tcagcagcag
    24901 cgcggcgtcg gcggcgctac gggccatcgg tccgccctgg tccaggctcg aggcgaaggc
    24961 gatcatcccg tggcgcgaga cgcggccgta ggtgggcttc agcccggtca ggccgcacag
    25021 cgctgcgggc tggcggatcg agccgccggt gtcggtcgcg gtggcgcccg gcgcgagccg
    25081 cgcggcgacc gcggctgcgg atccgcccga ggagccgccc ggtacgcgct cggtgtccca
    25141 ggggttgcgc accggaccgt agtaactggt ctcgttcgac gagcccatcg cgaactcgtc
    25201 catgttggtc ttgcccagcg tgacgcagcc ggcggcggcg aggcgctcga cgaccgtggc
    25261 gtcgtagggc gggatgaagt tgtccagcat gcgcgagccg caggaagtgc gcatgccgct
    25321 ggtgcagaag atgtccttgt gcgcgatcgg gacgccgcag agcgggccac cctcgccgcg
    25381 cgcgcgcagt tcgtcggcac gccgcgcggc ggcgagcgcg gcctcggggt cgaccgtgat
    25441 gtaggcattc agggtcgggt tcatccgggc gatgcgcgcg aggaagtgcg cggtgatctc
    25501 cgccgaggag aattcccggc gcgcgagtgc atcgcggatg gcgtggaggg agaggtcgtg
    25561 catgcggggc gtttcctgga cgttctgggg gcgcgcggtg ccggtttcct actcg
//
LOCUS       DAORUQ010000025        25278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1516_length_25278_cov_6.300083, whole genome shotgun sequence.
ACCESSION   DAORUQ010000025 DAORUQ010000000
VERSION     DAORUQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 25278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25278
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_25278_cov_6.300083"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLW13_04020"
     CDS             <1..771
                     /locus_tag="PLW13_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69567.1"
                     /translation="SVQLARAGCHVAVVDVNAEAGERTAAEIRALGRRALFLRVDVRE
                     RSAVQDMVAQVVAELGGLDVMVSGVGHPVHIGSVLDLGEEQWDEVFALDIKTTLFCAQ
                     AAAVSMVERGVRGRIVNVSSSSGIAGAPTIAAYGAAKAGVIHLTKSLALELAPYGIRV
                     NCLVPGTHRTEAMQASLDAGGQMADFIRAAGKATPLGRLGEPLETGGVAVFLASNLSA
                     YMTGHAVISDGGITHTTSRPPVGAPAVPRALAHIKGAQ"
     gene            773..1789
                     /locus_tag="PLW13_04025"
     CDS             773..1789
                     /locus_tag="PLW13_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69568.1"
                     /translation="MDILDFTRSVEVIDAHIHMQRSVAHTQELYHYFLYRGAARSGPA
                     NPPVIGTLDQVQAMMERTGVRHANVLMFTWSGMYYRDGQFTLPDDPADRARADAELRQ
                     RIARRICDYNEWTLQTALQNRNLSCFVGIDPVVMDERAMLAEIEDKIRRGAKGVKSQF
                     TDCGILANDRRLWPLYDYLQAHEIPLQMVCAEWAPGLNRPAHCAEALADFPNLRLIFS
                     HIGHGHHFGQGTDAEFLELAQKYPYVCGDLSLRLPEVIDGQIAPDALVAHLRRIGTDR
                     VFYGSNFSLNEMLRPGPAPSEFDFQMSETLKGLAALATLPLTEDERADIAGRNFRRWV
                     GLGR"
     gene            1876..3672
                     /locus_tag="PLW13_04030"
     CDS             1876..3672
                     /locus_tag="PLW13_04030"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQY69569.1"
                     /translation="MHIPREQTQAIIDAHRAAAGAMMMRTDFLVPDCFEQQVGRLAAR
                     TALAYEGERITWTELEQRSAHYGRVALASGLRPGDVGAVMIENRPEFFYAWLGLARIG
                     VIAGLINTSAQGVALAHAVRATQAKVLFIGDECMEHLCATEGLPREALTIVRVNDAAT
                     PQARRHPRAVDLEAVDIAGIDVEDFRALRAGMDNRRPCFYIFTSGTTGLPKAALISHG
                     RFLSVGTGWMSVMPLGENDVFYCALPLFHGAALMSLYSTALQSGGSVLIRRRFSVSRF
                     WADVQAWRVTVFQYVGEVVRYLTNVPSVPEEKDHSLRMIMGSGIGIDVWQRFKQRFGE
                     HVLVYEGWGATESNANMTNFDQKPGSCGRLPFPERSHIRMLRYDQGSDELMRDAGGRY
                     ILCKPGEVGELYGQIRRGNGEIVAPFEGYTTATDTAAKILQDVFEPGDAWWRSGDLFR
                     FDEDGYFYFVDRIGDTFRWKGENVSTTEVAQQLSIYADAESTNIYGVKVPGHEGRACM
                     AALALKPGHAFDPQAFYHCVTRTLPVYAQPMFVRVLSQADVTANFKLRKVRLREEGFD
                     TGLISDPLYGLDRERETYAPLDAALLARLCAA"
     gene            3690..4805
                     /locus_tag="PLW13_04035"
     CDS             3690..4805
                     /locus_tag="PLW13_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69570.1"
                     /translation="MQQQQDLEQGAAREALAEALLSGKAWEDFCDRLKAAGRFVQAVD
                     SIDRERDIAEGYRYLTRLARMGLGMILENGTPAAPSLLYQDPTLKSGGDNPDNMYYWG
                     RIRGRHSYRLDIGLKPNVDLSVIVYAGGLNKDGGRRTVGELTLAELEVDAGGRATVWL
                     GGDPREGNWLALAEDAGTLLIRETVADRRRANPSKFALSCLDGHPPPPLTPLEMAKSL
                     RSVSGFVDYSIRYFSEMAGKWQQTPNRFFATDTSTVGSSFADSRYQYPSCYFSLRDNE
                     ALLLEFTPPAGCFWNVVLSNHWFESLEYLYYPVHVNPLMHAFVPGEPVRIVIAARDPG
                     LAGHHWLDTTGHRQGILNLRFVNAPPQPLPALRVVEI"
     gene            4837..>5246
                     /locus_tag="PLW13_04040"
     CDS             4837..>5246
                     /locus_tag="PLW13_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69571.1"
                     /translation="MGGVLEGKVAIVTGASRGIGVGIAKRFAAEGAKVVITARTGDAG
                     RDAPLPGTLAEVAAAIRAGGGECLPVVADLAVPEDRARIVDETLAAWGGVDILVNNAA
                     RGFYGPTMEMSRKRLLLSFEINLYAPIDLMQRVVP"
     assembly_gap    5247..5346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5347..5895
                     /locus_tag="PLW13_04045"
     CDS             <5347..5895
                     /locus_tag="PLW13_04045"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HQY69572.1"
                     /translation="AVRAAFPEGSIGAIKAVTVAVAHNGSDIAFRLQWQDASADTGAE
                     GQDDTGFPDAAALLFPTGAKAPLMSMGAPGLPVNAWYWRADEPDRGRQVSAEGLGSSE
                     TVDRDLVKVQGGHRNGEWHVVIARAMQATASRPVAQLGAGGRIGVSFAIWEGSRGERA
                     GIKAFALGPHEGWHELVLDAIA"
     gene            5910..6947
                     /locus_tag="PLW13_04050"
     CDS             5910..6947
                     /locus_tag="PLW13_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit beta"
                     /protein_id="HQY69573.1"
                     /translation="MNASKRQLAMVMDLNKCLGCHTCTVACKTLWTQGEGMDYMWFNT
                     VNTVPGKGTPKNWEAMGGGFDASGNARPGQLPGRKDFGEAWNFNHDELYHGGKGGSAR
                     LEIQGEAPQWGPNWDEDEGGGAFPNSFHFYLPRICNHCTHPACKEACPRQAIEKREED
                     GIVTVDEDRCRGYRFCQAACPYKKIYFNHQKKVAQKCIFCFPRIDAGVAPACARQCPG
                     RVRFVGYRDDPEAPIHKLVDKWKVALPLHPEYGTEPNVFYVPPLGPASFGTDGDIDDS
                     TPRIPDEYLVGLFGPKVLDALATIKAEREKMRLTGSSELMQLLIAYEWKDMFGGFDQD
                     PAAASVVRWVE"
     gene            6981..9848
                     /locus_tag="PLW13_04055"
     CDS             6981..9848
                     /locus_tag="PLW13_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY69574.1"
                     /translation="MRSSSGISRRSFLQGAAAGAATLLLDLHNLKPAFADGRLPPAAR
                     EYRNYRDIYRKKWSWDRVGVGTHSCSNCASGGCSWNLYVRDGIVWREEQAAPYTQTNA
                     SVPDYSPRGCQKGASAAALYRSPARVRYPLKRVGARGENRWKRITWDEALDEISTALV
                     DTLSTRGGVGAYCENGNNSDFGPSWIAMVRFFNQLGIPVTENFAGVGDLMTGATTTLG
                     MPVPGASSDDWFRTDYFVNWFGNPVSTRIPDAHFMTEARYRGARIVSITPDFNPTAIH
                     ADLWISPRPGTDAALALAACKVILDEDLHDVPYIIEQTDLPLLVRSDTRQFLREADVV
                     AGGRQECFGWWDGKKQQVVWSNSSMAVAAEQRTLKLAPDDHPVLEPEGATATLLSGAE
                     VELRSGFSIMLDKLSAYDLASAAKITGVHANVIQRFAREFATAKSAMIYAGAAAGKIL
                     HGDLVQRSQILLAALTGNNGRAGGGWQEMTFFELDGKMLAAFVDHPSDLTPDDPAAIW
                     GALAEVGKQQAASPAYISGSMMMLMNGGTRDAQLNPAYNDPTLPRTPAQFLEEAIASG
                     QIHNVPGPELGKPDIIFNMFGNPFRHRRMGDQLENTLFRTAKLVVDITLRMDDTARHS
                     DILLPTAGPYEKLGFKYSIAFPPYLHLGDKVVEPLGEAKADWEILSLLMERVSAEATR
                     RKVTEVRTYRGVPYDLSRAYWRFSDKNRIGPREDEKLMRMILALSPATRGITLEGLRA
                     NKGAMRYTGVSQLGKMWCGTSDYRIDEPLLPYQDMVVKKLAWPTATGRQQFYVDHPFF
                     IEVGEEFPVHKEPPQSGGAYPLTMTCGHTRWSIHTMWRDLDLLLRLQRGEPVVFVNPD
                     DAARRGIADHDFAAFHNDVGEFVARVKLTPGMRPGQVHIYHAWLANQFLTGKSNDSVS
                     ASPVKVTQLAGKHGHLQNEVGWYEFTGNDRDTRVEMKKHVA"
     gene            9845..10504
                     /locus_tag="PLW13_04060"
     CDS             9845..10504
                     /locus_tag="PLW13_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HQY69575.1"
                     /translation="MNAVGTPAYPAAVASRSLVYAAFAAAFAYPDREGMEAIRSGVLA
                     GALRQLLGAVDPKLPEGVDWAALQDCGADDEFLPVEFTRLFAAGENGPCCPLEGRCYG
                     SAGMEATEDLVRFYDFFGLSLSEGQREEPDHLLTELEFLHYLAYQEAALATAGESTEG
                     LIRAQRDFITRHPGKWVPLLCSRLSLAQPPRFFSELANLLLRLLQIEVARLDREVSAL
                     P"
     gene            10650..11555
                     /locus_tag="PLW13_04065"
     CDS             10650..11555
                     /locus_tag="PLW13_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69576.1"
                     /translation="MSNRPESPDDASRRHFIAGTGAGLTGLALSATALAQSPGTADAG
                     TACRRYQGKVVLITGATSGIGEATAHAFAREGARIFFCGRREERGRQVEKAIRDSGGD
                     ATYMRADVREEAQVAAFVARCVEVYGRLDIAFNNAGITGYMGAMDELAVEGIGYYHDI
                     MRTNVDGVLFAMRHELPVMRAAGGGIIVNTGSSLSHKGSAHMAAYAASKHAVIGLTRS
                     AAAREAGNGIRVVSVSPGGTRTDIHRPYLPAGATQSGGGGGAGTPMGRIAEPAEIAAV
                     VLSVTDPACVFLNGDDVRADGAASA"
     gene            complement(11596..13524)
                     /locus_tag="PLW13_04070"
     CDS             complement(11596..13524)
                     /locus_tag="PLW13_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69577.1"
                     /translation="MNMPQTLKLSATAQTAALPITDHASLRAALRDADVPTLLMVLTH
                     FERDEALLDSFAPYIGSIFDVPKEIPEPMLTQLRERLFRVLTTARPGTDTSVDNALMR
                     RMLSVDVGEPVADEFIPMLMEQMGFDAPEARSARAGRHAPAPDFRVLVIGAGLTGLLA
                     AIKLREAGYSFTVIEKNPEVGGTWWENTYPGVAVDTPSHFYSYSFELNPDWSHYTPAG
                     PEFQQYLLRVADKYDLRRHVVFDTRVLGCTWDEAAQCWNVEIQDASGTRIERANAVMN
                     AHGPVNRWSWPQIPGLESFKGARMHTARWDHSVSLKGKRVALIGTGASAVQVGPAIAP
                     EVAHLTVFQRSRHWVMPNHYVEVPETVRWAQRHIPHYAEWFRFRAYWFAADGLYANIQ
                     IDPEWPHQDTSVSAYNEGVRQYVLQNYHAKLAKRPDLQEKLIPAYPVFAKRICMDINW
                     LNTLTRDNVTLEDNTLDHIVEDGIVLKDGRKIPVDVIICATGFDVSDMTGGLKIIGRG
                     GRNLREEWGEDDPRAYLGVTVPGYPNFFLSVGPNSAPNHAGGQNIVSETQVHYALECL
                     ELLNDKHAKALEPTQEATDAFNAAVDEKLAGLIWSRPDAKSYYKNKAGRIIMSSPYKL
                     VEYWLMTRGPKPEDYRIE"
     gene            complement(13560..14498)
                     /locus_tag="PLW13_04075"
     CDS             complement(13560..14498)
                     /locus_tag="PLW13_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69578.1"
                     /translation="MPIDPQIAALFANAPDWPPTRSQPIPQLREAVRASSTAFPKLEV
                     PLASIVDRTVPGPAGEIPVRVYTPTGNGPFPLISYFHGGGYVTGNLDTQDMIARALAF
                     GAGAVVVSVDYRLAPENPFPAGPDDCWAATQWVAAHAAELNGDAGRLAVAGDSAGANL
                     AAGVALRARDAGGPHIAAQIMFYGSCDHPSTETPASREFADGPILSADDVAYFWELYL
                     ANPATEQNHPLASPVRAADHRNLAPAFMGTAEIDPSRDHGEAYIRKLAAAGVSTKLVR
                     YPGMIHGFVSWLGVIPAAQQAIDDACAFLAAHWPAR"
     gene            14695..16629
                     /locus_tag="PLW13_04080"
     CDS             14695..16629
                     /locus_tag="PLW13_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HQY69579.1"
                     /translation="MSRIGFASRFMAPAALGVLLVACADSVPPPSGDGPVADWPHWGG
                     SEGGSRYSPLTQVTPQNVGQLKKAWTYHIGAIDAPEQSSPTFEGTPIVAGGRMFVCSG
                     MGKVAALDPETGKELWEYRYTADTTGSYLLNCRGLTHYSDGAEYAGECPGRVFAGTLD
                     GRLLALDAATGKLCAGFGDNGSLDLLKGLGKTQPGDFSISSPPVIVGGRIILGGRIPD
                     NMRVDVPAGVIRAFDVHTGALVWAWNSAPPGMEPEPGEPYVRGTPNAWAPLAVDSERK
                     LVFVPTGNASTDHTNIIRGDKDHFAASIVALDAITGQLKWHFQTVHKDHWDYDIPSQP
                     VLFPLQRDGVEIPALAQSTKQGHIFILNRETGEPLFPVEERPVSLTGAIEGERYSPTQ
                     PFPANPAFIVRHELTRDDIWGFTPWDKGKCREMFDNANWEGVFTPPSLRGTVYYPSFM
                     GATNWGGVSVDPVNNILVTNTTQVPAILRMYPRAEADRLMAAGKRMTPSIGAPYAHTM
                     EPMLSPLGAPCIRPPWGTLLAIDLKKGERLWEVPLGSTRDVAPFPLWFDWGVPNMGGS
                     LLTASGLAFIGATTDNFLRAFDVKTGQELWKARLPGGGQATPMTYRLSKDGKQYVVIA
                     AGGHKYLGTDISDALVAYAL"
     gene            16798..17613
                     /locus_tag="PLW13_04085"
     CDS             16798..17613
                     /locus_tag="PLW13_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQY69580.1"
                     /translation="MSTRRGIGLCYGSLCDVSAAELVRIAGANGFGGLMLPPFPAAQP
                     AEIAGFRALLRDNGIRRVVLDGVMAPLPRCAFVVEHGWTADQHFAVAEQFGVDCFNVP
                     HYRGDPATTVAEFADALGPFCERAAAARITVALEFLPGTGIPDLERALRIVEAVAAPN
                     LGFTLDTWHWARTRATLADIRALAPGVIKDFQISDRAANQDALPDSEQWGRLLPGEGT
                     LPLGEIIRAVRANAPDLTVNAEIFSEELRRRDPHDAARAIAESLGLVVDQALL"
     gene            complement(17702..18907)
                     /locus_tag="PLW13_04090"
     CDS             complement(17702..18907)
                     /locus_tag="PLW13_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69581.1"
                     /translation="MQQRLLGADAAAATVIDVDAHFEPGDEWLLPYPDLAKRLPKLDP
                     GALAVHAIVGDLLHDVPPAARPPFEQLVPPGVAVLYGQEKIAEKERRAEFEGKHQFQV
                     ANAQARLKWMDQQGIHIQNVICLAGIAYGVQVEDLGLRQEVIRAANTWLADTCAASNG
                     RLLPVSALEYSSLEWAVEELARMRRRGSRIFLIPAYPVNGVPPIHPSWDVLWEAAVKL
                     GMVPMLHTGFERMRFDPGWANLGGDVTLMRMLGSAQRHVAPNTLLNGMVYSGVFERFP
                     QLTVLLAEVGTGWLPYLYREIDDRISPTAELFLGKYRLPLKPSEYLARNVKATPLSGG
                     NDQPLARIVAELPPEMLVFSTDFPHFEGFADPADYFAGALAQSSAAERAAFAGGTMAN
                     VFARMGQPL"
     gene            18983..20185
                     /locus_tag="PLW13_04095"
     CDS             18983..20185
                     /locus_tag="PLW13_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQY69582.1"
                     /translation="MKRGCACRQRRYRGTQVDTDPALNQYFTRKKNIVASAAPSGIPR
                     PSPPGPAEVYLKSYHFSARASLDMPGSSMGIELRESETPQPGPGQVLLRIRASALNFA
                     DLTFLAGGLPNAEGLVPLLDGAGEIAALGPGVSRWLVGDRVIANPHQNWLAGEARPPS
                     YGQVLGGTVDGMLRDYAVLPETGLAAMPSHLSFEEAAALPCAGLTAWNSILGGPPNKR
                     PCVPGETVLVQGTGGVALFLLQFAKLAGCRAIALTSSQSKAELLRSMGADAVINYVET
                     PDWSRPVLELAGGNGVDLVVELGGPSTLPQSIAVTRPGGRIALIGVVGGLGSIDYVNL
                     LPIITKALTVFANSMGSLADLENMSRAIEIHGLRPTLDRTFGFEEAPEAFRHFAARSH
                     VGKVVISH"
     gene            20244..21326
                     /locus_tag="PLW13_04100"
     CDS             20244..21326
                     /locus_tag="PLW13_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69583.1"
                     /translation="MLTEARRQELSDLLRPVSPPRVLENVYTDDQRERLLDVVRQHGP
                     WKLIIAQHFASAEELIATGSGGVPEGVTPTLDMFLTPTFRGFYANYSALLYPEIHDCF
                     YNATFLELAKSYWGAKYAKPQMMLFNVNGPCGNTDPGHLDSPSFRGIRYENSPTWLCS
                     VMGKSGLFRDYLIKSAQVITWFSHDPSSGFTYWPDGPLKSPQRLMPPVYNRGVVVQNE
                     MLMHRGEANGAPEQQRPAGLDFTTTFSGDPADRDHWLLKTGDRVIARHHTKELRFLVH
                     WTAEVFEDYDELKKNMEGTHNLTHEQAIDMLIADVRSRGIRIETPSDPLHDPQFIRAL
                     NSAYDIGAPASYPEGAPVTPIYTHAA"
     gene            21377..21835
                     /locus_tag="PLW13_04105"
     CDS             21377..21835
                     /locus_tag="PLW13_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HQY69584.1"
                     /translation="MLLKIVVSQLMTPKGLAFDRFLVKWTGHSLMNRVFARNEGYAPH
                     PALLLVTRGSKTGQLREVAIPYFQLDGKLMVVGSRGGMPEDPFWVKNLRSDSEARIHI
                     RRKEKRVRARIAQGDERKALWPRLVTLVPTYARYQANTTREIPVVILDPA"
     gene            21922..22737
                     /locus_tag="PLW13_04110"
     CDS             21922..22737
                     /locus_tag="PLW13_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69585.1"
                     /translation="MDRLKGKVAIVTGAGRIGNIGLAICEAFLREGAKGVIGTDIRSD
                     EAMAIRERVERQFGKERFRLLEHDVTSPADWQRVVDAVVLEFGGLDVLVSNAGIAIHG
                     GIENTSLEDLRKVMAVNHDAMFLGMKSCLPALTESVKRFPGGGAIVNNISMSSYMPNA
                     NNIGYHVSKAAARMLTLCGATEFGPKKIRVNSVHPGLTITPLVRESFVDYVAAGLWET
                     EAAAEAAIAGMGPLNMASQPEDTAHAFVYLASEEARFVTGASIYHDGGIGQRY"
     gene            22749..23165
                     /locus_tag="PLW13_04115"
     CDS             22749..23165
                     /locus_tag="PLW13_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY69586.1"
                     /translation="MSEARKARFSFTKLIVNDEETMARYYHEVYGLNVIQRVQGESSA
                     AGEPFREIIMGPGDSMSPDESLVMFSFIGRPAPRDQECIIGFVTDDLDALVARIAANG
                     GKLVGPIKSMPEHGIRVVFATDPEGHLSENVQMLPA"
     gene            23369..>25278
                     /locus_tag="PLW13_04120"
     CDS             23369..>25278
                     /locus_tag="PLW13_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY69587.1"
                     /translation="MKHRINNMKMKRSTWLLRAAIAGSVMGVGSAGAWAQDGAQASGL
                     EEVVVTAQRREENIQSVPVAITAFDNNSLREQNITSPLDLNGKVPGVTVGNGGQQRNG
                     EVVTIRGQGQTYLSPVGVVNYFAEVPLIQSGVLANQGGPGTFWDLSSLQVLRGPQGTL
                     FGRNTTGGAVLLGPKKPAEEFSGYVQAQGGDYDNKEYEGAVNVPLIDDKLMVRLSYKD
                     VERDGFTEDVGPQPYGFNDVCQPMVARLGTIDIPCGHFGTHPRSPGFKGADYDNKDYW
                     HARVGVLWRPTDRIENYFVGYQSKSEDNGTGFIFSGAGPGMNVANLSGNLAYNITNPD
                     IFDPTITQDVLAVQNRLGARKTAMNQDQYTEIETEGYFNTTSIELSDNLTFRNILAYQ
                     TMQVNYSWDLDGSILPMLGQLDPVGAENTPVANDPFSRPGQRGSITDQSLLTEEAQLT
                     GTLLDERLDFVTGIYYSKQEPDGFNALGSFNAASYSPSTSAEIETTSTAIYAQGTLQL
                     GIISESLEKFSLTLGVRRTEDEEEGARFATNYYSDRVYTQLPDTYTIQPRTATLNSDE
                     PTWTAGLDYEMNEDVLLYGKITRGYKAGSFNQSGVSRMTSEPEFVTNYEIGAKTDFML
                     GEMPARLNANLF"
BASE COUNT         4293 a         8195 c         8569 g         4121 t
ORIGIN      
        1 tcggtgcaac tcgcgcgcgc cggttgtcac gtggcggtag tcgacgtgaa cgcggaggcg
       61 ggcgagcgca ccgcggccga gatccgcgcg ctcggccggc gcgcattgtt cctgcgcgtc
      121 gacgtgcgcg agcgcagcgc cgtgcaggac atggtcgcgc aggttgtggc cgagctcggc
      181 gggctcgacg tcatggtgag cggcgtgggg cacccggtgc acatcggttc ggtgctggac
      241 ctgggcgagg agcaatggga cgaggtgttc gcgctggaca tcaagaccac gctgttctgc
      301 gcccaggccg cggcggtctc gatggtcgaa cgcggcgtgc gcgggcgcat cgtcaacgtc
      361 agctcctcca gcggcattgc cggggcgcct accatcgccg cctacggtgc ggccaaggcc
      421 ggcgtgatcc acctcaccaa atcactggcg ctggagctcg cgccctacgg cattcgcgtg
      481 aactgcctgg tgccgggtac acaccggacc gaggcgatgc aggcgagcct ggacgcggga
      541 ggacagatgg cggacttcat ccgtgccgcg ggcaaggcga caccgcttgg acgacttggc
      601 gagcccctgg agacgggcgg cgtcgccgtt ttcctggcct cgaacctgtc ggcctacatg
      661 accgggcacg ccgtgatttc cgacggcggc atcacccaca cgaccagccg tccgccggta
      721 ggcgcgccgg ccgtgccgcg cgcgctcgcg cacatcaagg gagcgcagtg aaatggacat
      781 cctggatttc acccgctcgg tcgaggtgat cgacgcgcac atccacatgc agcgctcagt
      841 ggcgcacacg caggagcttt atcattactt cctgtatcgc ggcgcagcgc gcagcggacc
      901 ggccaacccg ccggtcatcg ggacgctcga ccaggtgcag gcgatgatgg agcgcaccgg
      961 cgtgcgccac gcaaatgtgc tgatgttcac gtggtccggc atgtattacc gcgacgggca
     1021 gttcacgctg cccgacgatc ctgccgatcg cgcgcgtgcc gatgcggagc tcagacagcg
     1081 catcgcgcgg cgtatctgcg actacaacga gtggacgttg cagacggcct tgcagaaccg
     1141 caacctcagc tgcttcgtcg gcatcgaccc ggtggtgatg gacgagcgag cgatgctggc
     1201 agagatcgag gacaagatcc ggcgcggcgc gaagggtgtg aagtcgcagt tcaccgactg
     1261 tggcatcctg gccaacgacc gccgcctgtg gccgctatac gattacctgc aggcgcacga
     1321 gatcccgctg caaatggtct gcgccgagtg ggccccgggg ctgaaccgcc cggcgcactg
     1381 cgccgaggcg ctcgccgact tcccgaacct gcggctgatc ttctcgcaca tcggccacgg
     1441 ccatcatttc gggcagggca cggacgcgga gttcctcgag ctcgcgcaga agtatccgta
     1501 tgtctgcggc gatctgtcgc tgcgcctgcc cgaggtgatc gacggccaga tcgcgcccga
     1561 cgcgctggtg gcgcacctgc gtcgtatcgg cacggaccgc gtgttctacg gcagcaactt
     1621 cagcctgaac gagatgctgc gtccgggccc ggcgccgagc gaattcgact tccagatgag
     1681 cgagacgctg aagggcctcg cggcgctggc gacgctgccc ctcaccgagg atgagcgcgc
     1741 cgacattgcc gggcgcaatt tccgccgctg ggtgggactg ggccgctgag gcgcatgcgc
     1801 gatgcgataa tggcgccctg aagcgccgcg cattgtgcgc tgcaacgacc gacctgagga
     1861 aaacggagaa cacctgtgca cattccgcga gagcagaccc aggccatcat cgacgcccac
     1921 cgcgctgccg ccggcgccat gatgatgcgc acggatttcc tggtgccgga ctgcttcgag
     1981 cagcaggtcg gtcgcctcgc cgcgcgcacg gcgctggcct acgaggggga acgcatcacg
     2041 tggaccgagc tcgaacagcg ctcggcacat tacgggcgcg tggcgctggc gagcggactg
     2101 cggccgggcg acgtcggcgc ggtgatgatc gagaaccgcc ccgagttctt ctatgcctgg
     2161 ctcggtcttg cgcgcatcgg cgtgatcgcg gggctgatca acaccagcgc gcagggcgtg
     2221 gcgctcgccc acgccgtgcg cgccacgcag gcgaaagtgc tgttcatcgg cgacgaatgc
     2281 atggagcatc tctgtgccac cgagggcctg ccgcgggaag ccctcacgat cgtgcgcgtc
     2341 aacgatgcgg cgacgccgca ggcgcggcgc catccgcgcg ccgtcgacct ggaggcggtc
     2401 gatatcgccg gcatcgatgt ggaggatttc agggcgctgc gcgcaggcat ggataaccgc
     2461 aggccgtgct tctacatctt cacctcgggt accacgggcc tgccgaaggc ggcactgatc
     2521 agccacggcc gctttctctc ggttggcacc ggctggatga gcgtgatgcc gctcggcgag
     2581 aacgacgtgt tctattgcgc actgccgttg ttccacggcg cggcgctgat gtcgctctac
     2641 agcaccgcgc tgcagtccgg tggcagcgtg ctgatccggc gccgcttcag cgtttcgcga
     2701 ttctgggcgg atgtgcaggc ctggcgcgtt acggtgttcc agtacgtcgg tgaggtggtg
     2761 cgctacctga ccaacgtgcc gtccgtgccg gaggagaagg accacagcct gcgcatgatc
     2821 atggggtccg gaatcggtat cgacgtgtgg cagcgcttca agcagcgttt cggcgagcat
     2881 gtgctcgtct acgaaggctg gggtgcgacc gaatcgaatg ccaacatgac gaacttcgac
     2941 cagaagccgg gcagctgcgg gcgcctcccg ttcccggagc gcagccacat ccgcatgctg
     3001 cgctatgacc agggcagcga cgagctgatg cgcgacgccg ggggtcgcta catcctgtgc
     3061 aagcccggcg aggtcggcga gctctacggg cagatccggc gtggcaacgg cgagatcgtc
     3121 gcacccttcg agggctatac caccgcgacc gataccgccg cgaagattct ccaggacgtg
     3181 ttcgagccgg gcgacgcctg gtggcgctcg ggcgacctgt tccgcttcga cgaggacggc
     3241 tacttctact tcgtggaccg gatcggcgac acgttccgct ggaagggcga gaacgtttcc
     3301 accaccgagg tcgcgcagca gctctcgatc tacgccgacg cggaaagcac caacatctac
     3361 ggcgtgaagg tgccgggcca cgaggggcgc gcgtgcatgg cggcgctcgc gctgaagccg
     3421 gggcacgcct tcgacccgca agctttctac cactgcgtca cgcggacgct cccggtctat
     3481 gcgcaaccga tgttcgttcg cgtgctgtca caggccgacg tgacggcgaa cttcaagctg
     3541 cgcaaggtgc ggctgcgcga ggagggcttc gatacggggc tgatcagcga tccgctctat
     3601 ggtcttgacc gtgagcgcga aacgtatgcg ccgctcgacg ccgcgctgct ggcgcgtctc
     3661 tgcgcggcat gatgccggga ggctcgcgga tgcagcagca acaggatctg gagcagggcg
     3721 cggcgcgtga ggcactggcc gaggcgctgc tgagcggcaa ggcgtgggag gacttctgcg
     3781 accggctgaa ggccgccgga cgtttcgtac aggccgtcga ttcgatcgat cgcgagcgcg
     3841 acatcgccga gggctaccgc tacctcacgc ggctggcgcg catgggcctc gggatgattc
     3901 tcgagaacgg cacgccggcc gcgccctcgc tgctgtacca ggatcccacg ctcaagtccg
     3961 gcggcgacaa cccggacaac atgtactact ggggccgcat ccgcgggcgc cacagctacc
     4021 ggctcgatat cgggctgaag ccgaacgtcg atctcagcgt gatcgtctac gccggcggtc
     4081 tgaacaagga cggcgggcgg cgcaccgtcg gcgagctcac gcttgctgaa ctcgaggtgg
     4141 atgccggggg gcgcgccacg gtctggctgg gcggcgatcc gcgcgagggc aactggctcg
     4201 cgctcgcgga ggacgcgggc acgctgctga tccgcgaaac cgttgcggac cggcgtcgtg
     4261 ccaacccgag caagttcgcg ctgagctgcc tcgacggcca tccgccgccg ccgctgacac
     4321 ccctcgagat ggcgaaatcc ctgcgcagcg tctctggctt cgtcgactac tcgatccgct
     4381 atttctcgga gatggccggg aagtggcagc agacgccgaa ccgcttcttc gccaccgaca
     4441 cttcgacggt cggcagcagc tttgccgatt cgcgctacca gtacccgagc tgctacttca
     4501 gcctgcgcga caacgaggcg ctgctgctgg agttcacgcc gcccgcgggt tgcttctgga
     4561 acgtggtgct gtcgaaccac tggttcgaat cgctggaata cctgtactac ccggtgcacg
     4621 tgaacccgct gatgcatgcc tttgtgccgg gcgagccggt acgcatcgtg atcgccgcgc
     4681 gtgatccggg actggcagga caccactggc tggataccac gggacaccgc cagggcatcc
     4741 tgaatctgcg tttcgtcaac gcgccaccgc agccgctgcc ggcgctgcgg gtggtggaaa
     4801 tctgaaccgg ccggcacagg cacgcaggag caatcgatgg gcggggtact cgaagggaag
     4861 gtggcgatcg tgaccggtgc gagccgcggc attggcgtcg gcatcgcgaa gcgtttcgcc
     4921 gcggaaggtg caaaggtggt catcaccgcg cgcaccgggg acgcaggccg cgacgccccg
     4981 ctgccgggca cgctggccga ggtcgccgcc gccatccgcg ccggcggcgg cgaatgcctg
     5041 ccggtggtcg ctgatctcgc ggtgccggag gaccgcgcgc gcatcgtgga cgagactctg
     5101 gcggcctggg gtggcgtgga catcctggtc aacaacgccg cgcgtggttt ctacggcccg
     5161 accatggaga tgtcgcgcaa gcggttgctg ctcagcttcg agatcaacct gtacgcgccc
     5221 atcgacctga tgcagcgcgt ggtgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnngcgg tgcgtgcggc ctttcccgag ggctccatcg gcgcgatcaa ggccgtgacc
     5401 gtggccgtgg cgcacaatgg cagcgacatc gcgtttcgcc tgcaatggca ggatgcctcg
     5461 gccgatactg gcgccgaggg gcaggacgac accggttttc ccgatgccgc cgcgctcctg
     5521 tttccgaccg gtgccaaggc ccccctgatg agcatgggcg cgcccggcct gcccgtgaat
     5581 gcctggtact ggcgtgccga cgaacccgac cggggcaggc aggtcagcgc cgaggggctc
     5641 ggtagcagcg agaccgtgga tcgcgacctc gtcaaggtgc agggtgggca tcgcaatggc
     5701 gaatggcacg tggtgatcgc gcgcgcaatg caggccacgg cgtcgcgacc ggtagcgcaa
     5761 ctgggcgccg gcggccgcat cggcgtgagc ttcgcaatct gggagggcag ccgtggcgag
     5821 cgcgcgggca tcaaggcctt cgcgctgggg ccgcacgagg gctggcacga actcgtactg
     5881 gacgccatcg cctgatcggg gagaaagaca tgaacgccag caaacgccag ctcgcgatgg
     5941 tgatggacct gaacaagtgt ctcggatgcc acacgtgtac cgtggcgtgc aagacgctgt
     6001 ggacccaggg cgaaggcatg gactacatgt ggttcaacac cgtcaatacc gtccctggca
     6061 agggcacgcc gaagaactgg gaggcgatgg gcggtggctt cgatgccagc ggcaacgcca
     6121 ggcccggcca gctgccgggt cgcaaggatt tcggcgaggc gtggaacttc aaccacgacg
     6181 agctctacca cgggggcaag ggcggtagcg cgcgcctgga gatccagggc gaggcaccgc
     6241 aatggggccc gaactgggac gaggacgagg gtggcggcgc cttccccaat tccttccact
     6301 tctacctgcc gcgcatctgc aaccactgca cccatccggc atgcaaggaa gcctgtccac
     6361 gccaggccat cgagaaacgc gaggaagacg gcatcgtcac ggtcgacgag gaccgttgcc
     6421 gcggctaccg cttctgccag gcggcctgcc cgtacaagaa gatctacttc aaccaccaga
     6481 agaaagtcgc gcagaaatgc atcttctgtt ttccgcgcat cgacgccggt gtcgcgccgg
     6541 cctgcgcgcg tcagtgcccg ggccgggtgc gctttgtggg ctatcgcgac gatcccgagg
     6601 cacccatcca caagctggtg gacaagtgga aagtggcgct gcccctgcac ccggaatacg
     6661 gcaccgagcc gaacgtcttc tacgtgccgc cgctgggtcc cgccagtttc ggtaccgatg
     6721 gcgatatcga cgacagcacg ccgcgcattc ccgatgaata cctcgtgggc ctcttcggcc
     6781 cgaaggttct ggatgcgctg gcgacgatca aggccgagcg cgagaagatg cgtctcaccg
     6841 gcagttcgga gctgatgcag ctgctgatcg cctacgagtg gaaggacatg ttcggcggct
     6901 tcgaccagga tcccgcggcg gcgtccgtcg tgcgctgggt cgaatagcgc gcagcggcca
     6961 agcgcgcgca ggaggctacg atgcgatctt catccggtat cagccggcgc agctttctcc
     7021 agggcgcggc ggcgggcgcg gccacgctgc tgctggatct gcacaacctg aaaccggcct
     7081 tcgccgacgg gcgcctgccg ccggccgcgc gggagtaccg caactaccgg gacatctacc
     7141 gcaagaaatg gagctgggac cgcgtcggag tgggaacgca ctcctgcagc aactgcgcga
     7201 gcggtggctg ctcgtggaac ctgtacgtgc gcgacggcat cgtgtggcgc gaggagcagg
     7261 cggcgcccta cacgcaaacc aatgcgagcg tgccggatta cagtccgcgc ggatgccaga
     7321 agggcgcctc ggccgctgcg ctctatcgca gcccggcccg ggtgcgctat ccgctcaagc
     7381 gcgtcggggc gaggggcgag aaccgctgga agcggatcac ctgggacgag gcgctggacg
     7441 agatctccac ggcgctggtc gatacgctgt ccacccgcgg cggcgtcggt gcctattgcg
     7501 agaacggcaa caactccgac ttcggtccga gctggatcgc gatggtgcgt ttcttcaacc
     7561 agctgggcat ccccgtcacc gagaacttcg ccggcgtcgg cgacctgatg accggcgcga
     7621 ccaccacgct gggcatgccg gtgccggggg cgtcgtcgga cgactggttc cgtaccgatt
     7681 atttcgtcaa ctggttcggc aacccggtat ccacgcgcat ccccgatgcc cacttcatga
     7741 ccgaggcgcg ctatcgcggc gcgagaatcg tatcgatcac gccggacttc aatccgaccg
     7801 ccatccacgc cgacctgtgg atctccccgc ggccgggcac cgacgcagcg ctggcgctcg
     7861 ctgcctgcaa ggtgatcctc gacgaagatc tccacgatgt gccgtacatc atcgagcaga
     7921 ccgacctgcc gctcctggtg cgcagcgaca cgcgccagtt cctgcgcgag gccgacgtgg
     7981 tggccggcgg acggcaggag tgtttcggct ggtgggacgg gaagaagcag caggtggtgt
     8041 ggtcgaacag ttccatggcg gtggccgcgg aacagcggac gctgaagctg gcgccggacg
     8101 atcacccggt gctggaaccg gagggagcta ccgcgaccct gctctcgggt gccgaggtgg
     8161 aattgcgcag cggcttctcg atcatgctcg acaagctctc ggcctacgac ctcgcgagtg
     8221 cggcgaagat caccggcgtc catgcgaacg tcatccagcg cttcgcgcgc gaattcgcca
     8281 ccgcgaaatc ggcgatgatc tatgccggtg ccgctgcggg caagatattg cacggcgacc
     8341 tggtgcagcg ctcgcagatc ctgctggcgg cgctcaccgg caacaacggc cgcgcgggtg
     8401 gcggctggca ggaaatgacg ttcttcgagc tcgacggcaa gatgctcgcc gcgttcgtgg
     8461 atcatccgag cgatctgaca cctgacgatc ccgcggccat ctggggcgcg ctggcggagg
     8521 tcggcaagca gcaggcggcg agcccggcgt atatctccgg gtccatgatg atgctgatga
     8581 atggcggcac gcgcgacgcg cagctgaacc ccgcctacaa cgaccccaca ttgccgcgca
     8641 cgcccgcgca gttcctcgag gaagcgatcg cctcgggcca gatccacaat gtgccgggcc
     8701 cggagctcgg caagcccgac atcatcttca acatgttcgg caacccgttc cgccatcggc
     8761 gcatgggcga ccagctggag aacacgctgt tcaggaccgc gaagctggtc gtggacatca
     8821 cgctgcgcat ggacgacacc gcgcggcatt ccgacattct gctgcccacg gccggtccct
     8881 acgaaaagct gggcttcaag tattcgatcg catttccgcc gtatctgcat ctcggcgaca
     8941 aggtggtcga gccgctgggc gaggcgaaag cggactggga gatcctctcg ctgctcatgg
     9001 agcgcgtgag cgccgaggcc acgcggcgca aggtcaccga ggtgcggacc tatcgcggcg
     9061 tgccctacga tctgtccagg gcgtactggc gcttcagcga caagaaccgc atcgggccgc
     9121 gcgaggacga gaagctcatg cgcatgatcc tcgcgctgtc accggcgacg cgcggcatca
     9181 cgctggaggg cttgcgcgcg aacaagggcg ccatgcgcta caccggcgtc agccagctgg
     9241 gcaagatgtg gtgcggcacg agcgactacc gcatcgacga acccctgttg ccttaccagg
     9301 acatggtggt gaagaagttg gcgtggccca cggccacggg gcgacagcaa ttctacgtgg
     9361 accacccgtt cttcatcgag gtcggcgagg aattcccggt gcacaaggaa cctccgcagt
     9421 ccggtggcgc gtatccgctc accatgacct gcggccacac gcgctggagc atccacacca
     9481 tgtggcgtga tctcgacctg ctcttgcgcc tgcagcgcgg cgagccggtg gtctttgtga
     9541 acccggacga tgccgcccgg cgcggcatcg ccgatcatga tttcgccgcg ttccacaacg
     9601 acgtcggcga gttcgtggcg cgcgtgaagc tgacgccggg catgcgaccc ggacaggtgc
     9661 acatctacca cgcctggctg gcgaaccagt tcctgaccgg gaagagcaac gattcggttt
     9721 ccgcgagccc ggtgaaagtg acgcagttgg cgggcaagca cggtcacctg cagaacgagg
     9781 tcggctggta cgagttcacc gggaacgacc gggatacccg cgtcgagatg aagaagcacg
     9841 tggcatgaac gcggtaggga caccggcgta ccccgcagcc gttgcctcgc gcagccttgt
     9901 ctacgcggcc ttcgccgccg cgtttgcgta cccggatcgt gaagggatgg aggcgattcg
     9961 atcgggcgtg ctggcgggcg ccctgcgcca gttgctgggc gcagtcgacc ctaaactgcc
    10021 cgagggagtg gactgggccg cgttgcagga ttgcggcgcg gacgatgagt tcctgccggt
    10081 cgagttcacg cgtctctttg ctgccggcga aaacgggccg tgctgccccc tggagggccg
    10141 ttgctacggc agcgccggca tggaggcaac cgaggatctg gtgcgtttct atgacttctt
    10201 cggcctgtcg ttgtccgagg ggcagcgcga ggagcccgat cacctcctga ccgagctgga
    10261 gttcctgcac tacctcgcgt accaggaagc cgcactcgct accgcgggcg agagcaccga
    10321 gggcctgata cgcgcgcagc gcgatttcat aacgcgtcac ccgggtaaat gggttccctt
    10381 gctgtgcagt cgcctctcgc tcgcacagcc tccgcgcttt ttcagcgaac tggcgaacct
    10441 gttgctgcgc ttgctgcaaa tcgaagtcgc gcgcctcgat cgtgaggtgt cggcattgcc
    10501 gtgagacgct gacggacctg ctcagctttg cggctcgatc agcaccttgg cttgtggcga
    10561 tgccgcgtcc gggggttcat gaattgaacc tgccccttcc ccgacggtcg taagacttgc
    10621 catccacgac aacaggagga agcatcacca tgtccaatcg tcccgaatcc cccgatgacg
    10681 ccagccgccg ccatttcatc gccggcaccg gcgccggtct caccggcctt gccctgagcg
    10741 ccaccgccct tgcgcaatcg ccgggaaccg cggatgcggg cacggcgtgc cgccgctacc
    10801 agggcaaggt ggtgttgatc accggcgcca catccggtat cggtgaggcc accgcccacg
    10861 cctttgcccg cgagggtgca cgaatctttt tctgtggccg gcgcgaggag cgtggcagac
    10921 aggtggagaa ggccatccgc gacagcggcg gtgacgcgac ctacatgcgt gccgatgtgc
    10981 gcgaggaggc gcaggtggcg gccttcgtgg ctcgctgcgt ggaggtctac ggccggctgg
    11041 acatcgcttt caacaatgcc ggcatcaccg gctacatggg tgccatggac gaattggcgg
    11101 tcgagggcat cggctactac cacgacatca tgcgcaccaa tgtcgatggc gtgttgtttg
    11161 ccatgcgcca cgaactgccg gtgatgcgtg ccgccggcgg cgggatcatc gtgaataccg
    11221 gctcgtcgct gtcacacaag ggctccgcgc acatggccgc ttacgccgcc tcgaaacacg
    11281 cggtgatcgg cctgacccgc tccgccgccg cccgcgaggc cgggaatggt atccgggtcg
    11341 tcagcgtttc ccccggcggc acgcgcacgg atatccaccg tccctatttg cccgcgggcg
    11401 ccacccagtc gggcggcgga ggcggcgccg gcacgcccat ggggcgaatc gccgagccgg
    11461 cggaaatcgc cgcggtggtg ctgagcgtca ccgatcccgc atgcgtgttt ctcaacggtg
    11521 acgatgtgcg ggccgacggg gcggcgtcgg cgtgatcgcg tatcgcactc cggtgcagcg
    11581 gcggcgtacc agccctcact cgatgcggta gtcctccggc ttcggcccgc gcgtcatcag
    11641 ccagtactcg accagcttgt aggggctcga catgatgatg cgcccggcct tgttcttgta
    11701 gtagctcttc gcgtccgggc gcgaccagat caatcctgca agtttttcgt cgaccgcggc
    11761 gttgaatgcg tcggtggcct cctgcgtggg ctccagcgcc ttcgcatgct tgtcgttcag
    11821 cagttcgagg cactccagcg cgtagtgcac ctgcgtctcg gagacgatgt tctgcccccc
    11881 ggcgtggttc ggcgcgctgt tgggcccgac gctgaggaag aagttcgggt agcccggcac
    11941 cgtgacgccg agataggcgc gcggatcgtc ctcgccccat tcctcgcgca ggttgcgccc
    12001 gccgcggccg atgatcttca gtccgccggt catgtccgag acatcgaagc ccgtggcgca
    12061 gatgatcacg tcgaccggta tcttgcggcc gtccttcagc acgatgccgt cctcgacgat
    12121 gtggtcgagc gtattgtcct cgagcgtcac gttgtcgcgc gtcagcgtgt tcagccagtt
    12181 gatgtccatg cagatgcgtt tggcgaacac cgggtatgcc ggaatgagct tttcctgcag
    12241 atccggccgc ttggcgagct tggcgtggta gttctgcagc acgtactggc gcacgccctc
    12301 gttgtaggcc gagaccgagg tgtcctggtg cggccactcg gggtcgatct ggatgttcgc
    12361 gtagagtccg tccgcggcga accagtaggc gcggaagcgg aaccactcgg cgtagtgcgg
    12421 gatgtggcgc tgcgcccagc gcacggtctc gggcacctcg acgtagtggt tcggcatcac
    12481 ccagtggcgc gagcgctgga acaccgtcag gtgtgccacc tcgggcgcga tcgccggtcc
    12541 cacctgcacg gcgctggcac cggtgcctat cagtgcgacg cgcttgccct tcagcgacac
    12601 cgagtggtcc cagcgagcgg tgtgcatccg cgcgcccttg aaggattcga ggccggggat
    12661 ctgcggccac gaccagcggt tcaccggccc gtgcgcattc atcacggcat tggcgcgctc
    12721 gatgcgcgtg ccgctggcgt cctggatctc gacgttccag cactgcgcgg cctcgtccca
    12781 ggtgcagccc agcacgcgcg tgtcgaacac cacgtgccgg cgcagatcgt acttgtcggc
    12841 cacgcgcagc aggtattgct ggaactcggg gccggccggc gtgtaatggc tccagtcggg
    12901 gttcagctcg aaggagtagg aatagaagtg gctcggcgtg tcgaccgcca cgccggggta
    12961 ggtgttctcc caccaggtgc cgcccacctc ggggttcttc tcgatcacgg tgaatgagta
    13021 gccggcctcg cgcagcttga tggccgcgag caggccggtg agcccggcgc cgatcaccag
    13081 cacgcggaag tccggggccg gcgcgtggcg gcccgcacgc gcgctgcgcg cctcgggcgc
    13141 atcgaagccc atctgttcca tcagcatcgg gatgaactcg tcggccaccg gctcgccgac
    13201 gtcgacgctc agcatccggc gcatcagcgc gttgtcgacc gaggtgtcgg tgccggggcg
    13261 cgccgtcgtc agcacccgga acaggcgctc gcgcagttgc gtcagcatcg gttcgggaat
    13321 ttccttcggc acgtcgaata tcgagccgat gtacggtgca aagctgtcga gcaaggcctc
    13381 gtcgcgctcg aagtgcgtga gcaccatcag cagcgtgggc acgtctgcat cgcgcagtgc
    13441 ggcgcgcagg cttgcgtggt cggtgatcgg cagcgcggcg gtttgcgccg tggcggagag
    13501 tttaagcgtt tgcggcatgt tcatgctcgg cctcgttatc gtggtgaatc cggatgcgct
    13561 cagcgcgcgg gccagtgggc ggccaggaag gcacaggcgt cgtctatggc ctgttgcgcg
    13621 gccgggatca cgccgagcca cgacacgaag ccgtggatca tgcccgggta gcgcaccagc
    13681 ttcgtgctca cgccagcggc cgccagcttg cgaatatagg cctcgccgtg atcgcgcgag
    13741 ggatcgatct ccgcggtacc catgaaggcc ggcgcaagat tgcgatgatc cgcggcgcgg
    13801 accggcgagg cgagcgggtg gttctgctcg gtggcgggat tggcgagata cagctcccag
    13861 aaatacgcca cgtcgtcggc cgagaggatc gggccgtcgg cgaactcgcg cgaggccggc
    13921 gtctcggtcg acggatggtc gcaggagccg tagaacatga tttgcgccgc aatgtgcggt
    13981 ccaccggcgt cgcgtgcacg caaggcgacg cctgccgcga gattcgcgcc cgcgctgtcg
    14041 cccgccaccg cgagccgtcc ggcatcgccg ttcagttccg cggcatgcgc ggctacccac
    14101 tgcgtggccg cccagcagtc gtccgggccg gcggggaagg ggttctcggg ggcgaggcgg
    14161 tagtcgaccg agaccaccac ggcgccggcg ccgaaggcca gtgcgcgcgc gatcatgtcc
    14221 tgggtgtcga ggttgccggt cacgtagccg ccgccgtgga agtagctgat cagcgggaag
    14281 gggccgttgc ccgtgggcgt gtacacgcgc accgggatct cgcccgcggg gccgggaacc
    14341 gtgcggtcga cgatcgaggc gagcggcacc tcgagcttcg ggaaggccgt ggaactggcg
    14401 cgcacggctt cgcgcagttg cgggatcggt tgcgagcgcg tcggcggcca atccggcgcg
    14461 ttggcgaaca gggcggcaat ctgggggtct atcggcatgg catgcattcc tcgcgggccg
    14521 gacgtgtccg gcctcatctt tttgtgtgtg gactggaaag cggttgacgg gcactctatc
    14581 acttgaacag gatatttatc ttaccgatac attaaacatt ccgtaaggcc cctgtccgtg
    14641 gcgcccgtca ggggcggtcg cgggttggag cggctgcgcg tcaggggagg cgatttgtcg
    14701 aggatcggat tcgcatcgag attcatggcg ccggcggcgc tgggtgtgct gctggttgcc
    14761 tgcgctgact ccgtgccgcc gccctcgggt gacggcccgg tcgcggactg gccccactgg
    14821 ggcggcagcg agggcggctc gcgctattcg cccctgacgc aggtcacgcc gcagaacgtg
    14881 ggccagttga agaaggcctg gacttaccac atcggcgcga tcgatgcgcc cgagcagtcc
    14941 tctccgacct tcgagggcac gccgatcgtg gccggcggac gcatgttcgt ctgctcgggc
    15001 atgggcaagg tggccgcact cgaccccgag accggcaagg agctgtggga gtaccgctac
    15061 accgcggaca ccacgggcag ctacctgctg aactgccgcg ggctcacgca ctattccgat
    15121 ggtgcggagt acgcgggcga gtgtccgggc cgcgtgttcg cgggcacgct cgacggccgc
    15181 ctgctggcac tggatgccgc caccggaaag ctgtgcgcgg gcttcggcga caacggctcg
    15241 ctcgacctgt tgaagggcct tggaaagacg cagccggggg acttctcgat ctcctcgccg
    15301 ccggtgatcg tcggcgggcg catcatcctc ggcgggcgca ttcccgacaa catgcgcgtg
    15361 gacgtaccgg ccggcgtgat ccgcgccttc gatgtgcaca ccggcgcgct ggtgtgggcg
    15421 tggaattccg cgccgcccgg catggaaccg gagcccggag agccctacgt gcgcggcacg
    15481 ccgaacgcgt gggcgccgct cgcggtcgac agcgagcgca agctggtgtt cgtgcccacg
    15541 ggcaacgcct ccaccgatca caccaacatc atccgcggcg acaaggacca cttcgccgcc
    15601 tcgatcgtgg cgctggacgc gatcaccggg caactgaagt ggcatttcca gaccgtgcac
    15661 aaggaccact gggattacga tattccctcg cagccggtgc tgttcccgct gcagcgcgac
    15721 ggcgtcgaga ttcccgcgct ggcgcaatcc accaaacagg ggcacatctt catcctgaac
    15781 cgcgagaccg gggagccgct gttcccggtc gaggagcgcc cggtgtcgct caccggcgcg
    15841 atcgaaggcg agcgatacag cccgacgcaa ccgttcccgg ccaatccggc gttcatcgtg
    15901 cgccacgagc tgacgcgcga cgacatctgg ggcttcacgc cgtgggacaa gggcaagtgc
    15961 cgcgaaatgt tcgacaacgc gaactgggaa ggcgtgttca cgccgccctc gctgcgcggc
    16021 accgtgtact acccgagctt catgggcgcg accaactggg gcggcgtgtc ggtcgacccg
    16081 gtcaacaaca tcctggtcac caacacgacg caggtgccgg cgatcctgcg catgtacccg
    16141 cgcgcggagg ccgaccggct gatggccgcg ggcaagcgca tgacgccgtc catcggcgct
    16201 ccctatgcgc atacgatgga gccgatgctg tcgccgctcg gcgcgccctg catccggccg
    16261 ccgtggggca cgctgctcgc gatcgacctg aagaagggcg agcggctgtg ggaagtgccg
    16321 ctgggctcca cgcgcgacgt ggcgcccttc ccgctgtggt tcgactgggg cgtgccgaac
    16381 atgggcggct cgctgctcac ggcatcgggg ctggcgttca tcggcgcgac caccgacaac
    16441 ttcctgcgcg ccttcgacgt gaagacgggg caggaactgt ggaaggcgcg gctgccgggc
    16501 ggcggccagg ccacgccgat gacttaccgg ctgagcaagg acggcaagca gtacgtggtg
    16561 atcgcggcgg gtggccacaa gtacctgggc accgatatca gcgatgcgct ggtcgcgtac
    16621 gcgttgtagg agcgggccat gcccgcgacc tgtcggagcc atgagcccct cctgccggcg
    16681 gcgggcggtt gttgtaggag cgcgccatgc gcgcgaccat cgcgggcatg gcccgctcct
    16741 acgtgaagga gaagctggcg atggacgcat tccggcgcga ccgacaagga gacgaatatg
    16801 tccacgcgac gaggcatcgg actctgctac ggcagcctgt gcgacgtcag cgcggctgag
    16861 ttggtgcgta tcgccggcgc caacggcttc ggcggcctga tgctcccgcc ttttccagcg
    16921 gcgcagcccg cggagatcgc gggtttccgc gcgctgctgc gtgataacgg tatccgccgc
    16981 gtggtgctcg acggcgtcat ggccccgctg ccgcgctgcg ctttcgttgt cgagcacggc
    17041 tggaccgcgg accagcactt cgcggtggcg gagcagtttg gtgtcgactg cttcaacgtg
    17101 ccgcattacc gcggcgatcc ggcgaccacc gtggcggaat tcgccgacgc gctcggtccc
    17161 ttctgcgagc gcgccgcggc cgcgcgcatc acggtggcgc tggagttcct gccgggcacc
    17221 ggcataccgg atctcgagcg tgcgctgcgc atcgtggaag cggtggctgc gccgaacctc
    17281 ggcttcacgc tggacacctg gcactgggcg cgcacgcgcg cgacgctggc cgatatccgc
    17341 gcgctcgcgc cgggcgtgat caaggatttc cagatcagcg accgcgcggc gaaccaggac
    17401 gcattgccgg attccgagca gtggggacga ctgctgccgg gcgaggggac gttgccgcta
    17461 ggggagatca tccgcgctgt gcgcgccaat gcgccggatc tcacggtcaa cgcggagatc
    17521 ttttccgagg agctacggcg gcgagatccg catgacgcgg cgcgcgcgat cgccgagtcc
    17581 cttggactgg ttgtggatca ggcgttgctg taggtcgcca ttcattgcga cggcggcatt
    17641 cgggcaggca cctgtcggca cgaatgccga accacagcag gatgtcgggc tgaagcccga
    17701 cctacagcgg ctgtcccatt cgcgcgaaca cgttcgccat cgtgccgccc gcgaaggccg
    17761 cgcgctcggc cgcggagctt tgggccagcg cgccggcgaa ataatcggcc ggatccgcga
    17821 agccttcgaa gtgcgggaag tccgtggaga acaccagcat ctccggcggt agctcggcaa
    17881 cgatgcgcgc cagcggctgg tcgttgccgc cactcagcgg ggtcgccttc acgttgcgcg
    17941 cgaggtactc gctcggtttc agcggcaggc ggtacttgcc gaggaacagt tccgcggtcg
    18001 gcgagatgcg gtcgtcgatt tcccggtaca ggtacggcag ccagccggtg cccacttcgg
    18061 ccagcagcac ggtgagctgc gggaagcgtt cgaacacgcc gctgtagacc atgccgttca
    18121 gcagggtgtt cggcgccacg tggcgctggg cactgcccag catgcgcatc agcgtgacgt
    18181 cgccgccgag gttcgcccag ccgggatcga agcgcatgcg ctcgaagccc gtgtgcagca
    18241 tcggcaccat gccgagcttc accgcggcct cccacagcac gtcccagctc ggatggatcg
    18301 gcggcacgcc gttcaccggg taggccggga tcaggaagat gcgcgagccg cgccggcgca
    18361 tgcgcgcgag ttcctcgacg gcccattcga gcgagctgta ttcgagcgcg ctcaccggca
    18421 gcagtcgccc gttcgacgcg gcacaggtgt cggccagcca ggtgttcgcc gcgcggatca
    18481 cttcctggcg caggcccagg tcctcgacct gcaccccgta ggcgatgccg gccaggcaga
    18541 tcacgttctg gatatggatg ccctgctggt ccatccactt caggcgcgcc tgggcattgg
    18601 cgacctggaa ctggtgcttg ccctcgaact ccgcgcgacg ctccttctcg gcgattttct
    18661 cctgcccgta cagcacggcg acgccgggcg gcaccagctg ctcgaacggc ggccgcgcgg
    18721 ccggcggcac gtcgtggagc aggtcgccca cgatcgcgtg caccgccaga gcgcccggat
    18781 cgagcttcgg cagacgtttc gcgaggtcgg ggtagggcag cagccattca tcgcccggtt
    18841 cgaaatgggc gtcgacgtcg atcacggtcg ccgccgcggc atcggcgccg agtaagcgtt
    18901 gttgcatgga tccgctgatg gcttgcatgt tcggggtgtt ccctctggga gacgccgcgg
    18961 cagtcggcgt ggttcgctgt cattgaagcg cggttgcgca tgccggcagc gccgctaccg
    19021 cggcactcag gttgacacgg atcccgccct caaccaatac tttacgcgta agaaaaatat
    19081 cgtggcaagt gcggcgccgt ccggaattcc gcgtccgtct ccaccagggc ccgccgaggt
    19141 ttatttgaag agttaccatt tcagcgcacg cgcaagcctc gatatgcccg gctcgagcat
    19201 gggcatcgag ttgcgcgaat cggaaacgcc gcagcccggc cctggtcagg tgctgctgcg
    19261 gatccgcgcc agtgctctga attttgccga tctcaccttc ctcgctggcg ggctgcccaa
    19321 tgccgaaggg ctggtcccgc tgctcgacgg cgccggcgaa atcgctgcgc tcggcccggg
    19381 cgtgagccgc tggctggtcg gggaccgcgt gatcgccaac ccgcaccaga actggctggc
    19441 cggcgaggcg cgcccgccga gctacggcca ggtgctgggc ggcacggtag acgggatgct
    19501 ccgcgactat gccgttctgc cggagaccgg cctggccgcc atgccttcgc acctgtcctt
    19561 cgaagaggcc gcggcgctgc cctgcgccgg cctgaccgcg tggaacagca tcctgggcgg
    19621 tccgccgaac aagcgcccct gcgtgcccgg cgaaaccgtg ctggtccagg gcaccggcgg
    19681 tgtggccctg ttcctgcttc agttcgcgaa gctcgcgggc tgccgcgcca tcgcgctgac
    19741 ctcgagtcag tcaaaggccg aattgctgcg cagcatgggc gccgatgcgg tgatcaacta
    19801 tgtcgagacg ccggactgga gccgaccggt gctggaactc gcgggcggca acggtgtcga
    19861 tctggtcgtg gaactcggcg ggccgagcac gttgccgcag tcgatcgccg tcacgcgtcc
    19921 cggcggacgg atcgcgctga tcggcgtcgt gggcggcctg gggagtatcg attacgtgaa
    19981 cctgctgccc atcatcacca aggcgctgac ggtgttcgcg aactcgatgg gcagcctggc
    20041 cgacctggag aacatgtcga gggccatcga gatccacggg ttgaggccga ccctggatcg
    20101 cacgttcggt ttcgaggagg cgcccgaggc cttccgtcac ttcgccgcca ggagccacgt
    20161 cggcaaggtc gtgatatcgc attgatcgcg ccggatcccc ggcgcgagac acctccaccc
    20221 acgcatcatc gaaggagaac cccatgttga ccgaagcccg ccggcaggaa ctctcggacc
    20281 tgttgcgccc ggtgtcgcca ccgcgcgtac tggagaatgt gtacacggat gaccagcgcg
    20341 agcgcctgct cgacgtggtg cggcagcacg ggccgtggaa gctgatcatc gcgcagcatt
    20401 tcgcctccgc cgaggagttg atcgccacgg ggtcgggagg ggttcccgaa ggcgtgacgc
    20461 ccacgctgga catgttcctg acgccgacct tccgcggttt ctacgcgaac tactcggcac
    20521 tgctctatcc cgagatccac gactgcttct acaacgcgac attcctggaa ctcgcgaagt
    20581 cctactgggg tgcgaaatac gccaaaccgc agatgatgct gttcaacgtc aacggcccct
    20641 gcggcaacac ggaccccggt cacctggact caccgagctt tcgcggcatc cgctacgaga
    20701 actcgccgac ctggctgtgc agcgtgatgg gcaaatcggg cctgttcagg gactacctga
    20761 tcaagtccgc gcaggtcatc acctggttct cgcacgaccc gagcagcgga ttcacctact
    20821 ggcccgacgg accgttgaag tcgccgcagc gcctgatgcc gccggtctac aaccgcggcg
    20881 tggtggtgca gaacgagatg ctgatgcatc gtggcgaagc caacggcgcg ccggagcagc
    20941 agcgcccggc cgggctcgac ttcacgacca ccttctccgg cgatcccgcg gaccgcgatc
    21001 actggctgct gaagacaggt gaccgggtga tcgcgcgcca tcacacgaag gagctgcgct
    21061 tcctggttca ctggactgcc gaagtgttcg aggactacga cgagctgaag aaaaacatgg
    21121 aaggcacgca caacctgacg catgagcagg ccatcgacat gctgatagcg gatgtgcgct
    21181 ccaggggtat ccggatcgag acgcccagcg atccgctgca cgatccgcag ttcatccggg
    21241 ccctgaactc ggcctatgac atcggcgcgc cggccagcta ccccgagggg gcgccggtca
    21301 cgcccattta cacgcacgct gcctgagcct gcgctagtaa tccatcaact gaatcacgaa
    21361 caaagacagg tttcccatgc tactcaagat agtggtatcg caattgatga cgcccaaggg
    21421 actggctttt gaccgctttc tggtgaagtg gacgggccac tccctgatga accgtgtttt
    21481 cgccaggaat gagggttacg caccacatcc ggccctgttg ctggtaaccc ggggtagcaa
    21541 gacgggccaa ctccgggaag tggctatccc ctactttcaa ctggacggaa agttgatggt
    21601 cgtgggatcc aggggtggca tgccggaaga tccattctgg gtcaagaacc tgcgtagcga
    21661 ctccgaggcg aggattcata tccggaggaa ggagaagcgg gtgcgggcgc gtatcgccca
    21721 gggcgatgag cgaaaagccc tctggccccg gttagtcacc ttggtgccca cctacgcccg
    21781 gtaccaggcg aatacgacgc gggagatccc tgtggtcatc ctggatccgg cgtgaccggc
    21841 agtttcatcg ccggctgcgt ggtcttcgcc agcggcggca tggtgccgca ctgatcgtca
    21901 ctacaacacg aggacttggg gatggatcga ctgaagggta aggtcgcgat cgtgacaggc
    21961 gctggccgca tcggcaacat cggtcttgcg atctgcgagg ccttcctgcg cgagggggcc
    22021 aagggcgtca tcggcacgga cattcgcagc gacgaggcga tggcgatccg cgagcgcgtc
    22081 gagcggcagt tcggcaagga gcgcttcagg ctgctcgagc acgacgtgac cagccccgcc
    22141 gactggcagc gagtggtcga tgcggtggtc ctggagttcg gcggcctcga tgtgctggtg
    22201 agcaacgccg gcatcgcgat acatggcggc atcgagaaca cctcgctcga ggatctgcgc
    22261 aaggtgatgg cggtcaacca cgacgcgatg ttcctcggca tgaagagctg ccttcccgcg
    22321 ctcacggaga gcgtgaaacg cttccccggc ggcggcgcca tcgtcaacaa catctcgatg
    22381 tcgtcctaca tgccgaatgc gaacaacatc ggttaccacg tttcgaaggc ggcggcgcgc
    22441 atgctgacgc tgtgcggggc gacggagttc gggccaaaga agatccgcgt caattcggtg
    22501 catcccgggc tcacgatcac cccgctggtg cgcgagtcct ttgtcgacta cgtggctgcc
    22561 ggactgtggg aaacggaggc ggcggcagag gctgcgatcg ccggcatggg cccgctgaac
    22621 atggcgagcc agccggaaga cacggcgcat gccttcgtct atttggcgtc cgaggaggcc
    22681 cgcttcgtga ccggcgcctc gatctaccac gacggcggca tcgggcagcg atactgagga
    22741 gagaccggat gagtgaagcc cgcaaggcaa ggttctcgtt caccaagctg atcgtgaacg
    22801 acgaagagac gatggcccgg tactaccacg aggtgtacgg gctgaatgtg atacagcgcg
    22861 tgcagggtga atccagcgcc gctggcgagc cgttccgcga gatcatcatg gggccgggcg
    22921 actcgatgtc gcccgatgaa tccctggtga tgttcagttt catcgggcgc cctgcgccgc
    22981 gcgaccagga gtgcattatc ggcttcgtca cggacgacct cgacgcgctg gtcgcgcgca
    23041 tcgccgccaa cggcggcaag ctggtcggcc cgatcaagtc gatgcccgag cacggcatcc
    23101 gcgtggtgtt cgccacggac cccgaggggc acctgagcga gaacgtgcag atgctgccgg
    23161 cctgagcgcg cgggcggaca gccgggaaat gccgctttac aaacagatag ccaacaatta
    23221 acttatcgat acgtttgtgg cggggaagct ggcgatgcgc cagtgtcgat caaggccaca
    23281 tgcgtcagaa caacaagaca gggcaacgaa tcacacacgg ccgttgccgc ccgcaaccag
    23341 accgacaacc acctccggga gagaccagat gaaacacagg atcaacaaca tgaagatgaa
    23401 acgttccacc tggttgctgc gggcagcgat tgccggcagc gtgatgggcg tcggcagcgc
    23461 cggcgcctgg gcgcaggacg gcgcgcaggc gtccggtctg gaggaggtgg tagtcaccgc
    23521 gcaacggcgc gaggagaaca tccagtccgt tccggtcgcg atcacggcct tcgacaacaa
    23581 cagcctgcgc gagcagaaca tcaccagccc gctggacctg aacggcaagg tgcccggcgt
    23641 cactgtcggc aacggtggcc agcaacgcaa cggcgaagtc gtgacgattc gcggccaggg
    23701 ccagacctat ctgtcgcccg tcggcgtggt gaactacttt gcggaagtgc cgctgatcca
    23761 gagtggcgtc ctggccaacc agggcggccc cggcacgttc tgggacctga gctcgctgca
    23821 ggtgctgcgc ggaccgcaag gcacgctgtt cggccgcaac accaccggcg gcgcggtact
    23881 gctggggccg aagaagcccg cggaggaatt ctccggctat gtgcaggccc agggcggcga
    23941 ctacgacaac aaggaatacg agggcgccgt caacgtaccc ttgatcgatg acaagctgat
    24001 ggtgcgcctg tcatacaagg acgtggagcg cgacgggttt accgaggacg tgggtccgca
    24061 gccttacggt ttcaacgatg tctgccagcc gatggttgcg cggctgggaa ccatcgacat
    24121 accgtgcggc catttcggca cccacccgcg ctcgcccggc ttcaagggcg cggactatga
    24181 caacaaggat tactggcatg cgcgcgtcgg tgtgctgtgg cgtccgaccg accgcatcga
    24241 aaactatttc gtcggctatc aatccaagtc cgaggacaac ggcaccggct tcatcttcag
    24301 cggtgccggc cccggcatga acgtcgcgaa cctgtccggc aacctggcgt acaacattac
    24361 gaatcccgat atcttcgacc cgacgatcac ccaggatgtg ctcgcggtcc agaaccggct
    24421 cggggcgcgc aagaccgcca tgaaccagga ccagtacacc gagatcgaga ccgaaggcta
    24481 tttcaacacg acgtcgatcg aactgagcga caacctgacg ttccgcaata tcctcgccta
    24541 ccagacgatg caggtgaact acagctggga cctcgatggt tcgatcctgc cgatgctggg
    24601 ccagctggac cccgtcggtg ccgagaacac cccggtcgcg aacgacccct tctcccgtcc
    24661 gggccagagg ggcagcatca cagaccagtc gctgctgacc gaggaagcgc aactgacggg
    24721 aaccctgctc gacgagcgac tggatttcgt caccggcatc tactactcga agcaggagcc
    24781 ggacggcttc aacgccctcg gctcgttcaa tgcggccagc tatagcccca gcaccagcgc
    24841 cgagatcgag accaccagca cggccatata cgcccagggc acgctgcagc tcggcatcat
    24901 ttccgagtcg ctcgagaagt tcagcctgac gctgggcgtg cgacgcaccg aggacgagga
    24961 ggaaggcgcg cgtttcgcca ccaactacta cagcgatcgc gtgtacaccc aattgccgga
    25021 tacctacacg atacaaccgc gcaccgcgac cctgaactcc gacgagccga cctggacggc
    25081 gggcctcgac tacgagatga acgaagacgt gctgctctac ggcaagatca cgcgtggtta
    25141 caaggcgggc agcttcaacc agtccggcgt gagccggatg acctcggaac cggaattcgt
    25201 gaccaactac gaaatcggcg cgaagaccga tttcatgctg ggcgagatgc cggcgcgcct
    25261 caatgccaac ctgttcag
//
LOCUS       DAORUQ010000026        22948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1821_length_22948_cov_6.125191, whole genome shotgun sequence.
ACCESSION   DAORUQ010000026 DAORUQ010000000
VERSION     DAORUQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 22948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22948
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1821_length_22948_cov_6.125191"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1212)
                     /locus_tag="PLW13_04125"
     CDS             complement(148..1212)
                     /locus_tag="PLW13_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQY69588.1"
                     /translation="MKAAVYREAGKPLVLETLPDPVPGPEDVIIKVHRCGICGTDLHM
                     TEGHQWQFSPGTVPGHEYSGEIVEVGANVTGFRTGDLITALPSTGCGNCEACHHGNLA
                     LCHNAPGVMGGYAEMMRIPASVAIKLPATLSAADGALIEPLAVGLYGVRMSCVQPGDR
                     VLVMGAGAVALCTIYWARRLGAGRIVATSRSARRQALALEMGADAFVHCGENESDGII
                     AALGGQPDIVFECVGTPGFLMKGIQHARTLGKVVSMGFCTSPDQVVPAVAGYKGVSLQ
                     FPVGYALKDFHYVADVMDAGHVDPKMLISSVVTLDELPTVFEQLRGPNNETKVQVAPL
                     ALQERLGSRPQAWAATIPAG"
     gene            complement(1209..2420)
                     /locus_tag="PLW13_04130"
     CDS             complement(1209..2420)
                     /locus_tag="PLW13_04130"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY69589.1"
                     /translation="MDDRYAAPGVFATALDQLHELVAKEVGSTDFGSDDYLPGLRVLL
                     QSMDYDPRFSERGRHYAWGTVVGVLKGRAQAIRSMAEHPGFDRHAIPSPVVITGMPRT
                     GTTALHKLMAVDQRFQGLQTWLLDSPMPRPPRETWTDYPAFHRTAATLEARFRAAPKK
                     RAAHNMAAEEVDECCLVLRQGFVSNLWTVGWSAATYDAWWQCQSEESSYRHYYRCLQL
                     IGSTEPDKRWLLKNPGHIENLDLLFAVFPDAKVIQTHRDPAKAVPSLCSLLMPLHPIV
                     EEGRYEQRAHNMLAREVAKWANALRKAQRVREAHPGQVLDLVHADLHRDPMAVVERIY
                     GFIGEDLRPEARAAMLQRIADDPERQHGTHDYDIADFGMTEAEIRERFGDYTSRFDLA
                     PKGPATAGGPK"
     gene            complement(2427..>3182)
                     /locus_tag="PLW13_04135"
     CDS             complement(2427..>3182)
                     /locus_tag="PLW13_04135"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69590.1"
                     /translation="GRVAIVTGGGTGIGLATARQLARRGAAVVVASRTADELERAAAR
                     VREESGARCLAVPTDVKDEEQVTRLVQRTLDEFGRIDILVNNAGGSRLGPLAGLGSRA
                     WDASFDLNVRAAYLCTHAVGPHMIAQRSGAIVNISSNAGISGVKGGAHYSAAKAALQM
                     FTTVTAAEWGRHGIRANCVAAGMIASERAAAAWEVAKLDMSNLTTIPLRRPGTPDEVA
                     NMIVFFASDAASYITGQTVAVNGGPSMSGIPDE"
     assembly_gap    3183..3282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3370..4548)
                     /locus_tag="PLW13_04140"
     CDS             complement(3370..4548)
                     /locus_tag="PLW13_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69591.1"
                     /translation="MDTRIGPSILEYLGALEDAVAELANTWHPEHADYRADVYRQTMM
                     SLSYSYFAYFHATPEHPDWAPLWNPVFTLQPNPDDIYLYSPIRGDLKYRVSGNRGTVK
                     ILSFTTQRFMSGMVDDIAQIGGHNEIDDQDIVVEPGGEFEVVFSVERPAGHAGNWAPI
                     HPQATGMMVRLRSYDWENEIDPTLSIECLDPVPAKPRLAPGEIIARIREMAKFPARKT
                     RSYYRLQNGVKERVGFNVFEPVRMKGALAKQVYWPACFQLEEDEALIIETDLPERRPY
                     WNIQLNDPYFNALEYVYRLSSLNGHTARASSDGRFRAVIALEDPGVPNWLDPAGYREG
                     GIYGRWFDCDTNPTPVIRRVKLAELRHHLPADTPVVTPQQRAEQLRRRVRACQRRRRW
                     "
     gene            complement(4586..5086)
                     /locus_tag="PLW13_04145"
     CDS             complement(4586..5086)
                     /locus_tag="PLW13_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69592.1"
                     /translation="MNRPDATFDPAAGLLRAEQFQVAYATNDIERARDIFGERYGISA
                     FRRLEGQLPAGGHIHVELAWVGGTMYELLTANGPGSQLYTGRLPADEFAIRHHHLGFL
                     IRNGDEWNALLTEIARKGWNMPSRSSNPGFMQSCFVEAPELGHYCEYLFPEPAGLAFF
                     ESVPGN"
     gene            5367..6032
                     /locus_tag="PLW13_04150"
     CDS             5367..6032
                     /locus_tag="PLW13_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69593.1"
                     /translation="MRRKKRRSPGEIRERLIDAAREEFKRCGFVGATTAAIARNADVT
                     EAQLFRCFESKAALFREAVFERLNQHFSEFNARYPTDVSSRANVRDRERRYITELQQF
                     LDEHSKLLLSLVAAQSFTSGSLQGVGEIESLRHYFERGAATMSGRIGEDPPLDPKLLV
                     RVSFAAVLGCVIFKDWIFPAGLASDDDINAAVIDFVIDGISINSDPGTKEPVRRSGKG
                     RAT"
     gene            6029..6193
                     /locus_tag="PLW13_04155"
     CDS             6029..6193
                     /locus_tag="PLW13_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69594.1"
                     /translation="MTPANTQLVRDFFTALASGNVPDDLLTPDMSLWTVNGGTSDEAR
                     FQGAMKKAAG"
     gene            6224..8527
                     /locus_tag="PLW13_04160"
     CDS             6224..8527
                     /locus_tag="PLW13_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY69595.1"
                     /translation="MQTRAKSFCRNCGALCSMELTIEDGKLAGVTADGSASPYGAYMC
                     PKGRASLDFHNGAEGRLLNSLRREASGAFSELPAAQALDEIAARLAALIAEHGPRSVA
                     FYHGTGAYRSVLGALLERAFASSLGSPNFFSTMTIDQSAKWVTAGRMGVMASGKPSTR
                     DIDLAVIVGNNPLVSHQTYPFGPGESGAPGKSFAAARARGARIVVVDPRRTETARYAD
                     LLIQPLPGHDAALFAAVAHILLRDGTCNTAFCERFVTQLDRLAEAVAPFTPELVARRA
                     DVPVEQIEQLARWLGEAERPFVGSGSGPSMSAHSNLNDHMIETVNALVGGYRRAGDLV
                     RNPGTLKPRVCIETVVPPQRSWERGVKCRTADIGALYGEFPTALLPQEILAPGPDRIR
                     ALIVFGGNPLTALGDPARAEPAFADLELLVSLDARMNETARMAHYVIATSQHFERHDL
                     SIPGDSLFPEAFAQYAPPVVPGPAGTIHDWEFFWGVASRLKLPLTLKYWNYGLSYDAI
                     PGGLPLDMERAPHPEDMMRYLCEHGDVSFEELRANPGGVRPVRPPQYVKAAPDNGARL
                     ELCPPDVAAELHAVLEEVVDDRFKYRLTCRRILDAMNSAYRDSPQTIRKYPLNWAYMN
                     PDDMRDDGIDDNELIEIQSESGRILGVARAESQLRRGVVSMTHLYGSLAPSTDPYAQR
                     GSHTGRLTSLQKHLEPINFMPRFSGIPVNIRRAGGDVPATAPLATILISPVRRRLPPP
                     GDSRALRANTCRARRSRRSRCNPRAPP"
     gene            complement(8717..10183)
                     /locus_tag="PLW13_04165"
     CDS             complement(8717..10183)
                     /locus_tag="PLW13_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY69596.1"
                     /translation="MEMLPEARLYIDGKLRAAAGNRTFDNICPWTGEVIGKSADASAD
                     DVNDAIAAARRAFDQTDWSQRHEERFALMKKYRDLLFANRDRLVQIVRLEAGSSIGAA
                     YRAQVDRALSGMDDVLACFPSVTWEEDRGRRNEMGFDSDRIVVHEPLGVVGAISPWNV
                     PLYVNVGKVVPALLAGCTVVLKPAPDTPATAAFMGELAVEAGFPPGVFNVITSADPVM
                     AGEALVKDPRVDVISFTGSTGVGKRIMETAAATLKRVFLELGGKSANIILDDAPDYAM
                     SVMQSMVIFHAGQGCAIPTRLLVPRSRYDEAVKALEMAYAGFATKWGDFDDPTQVMGP
                     VISARQRDRVMEYIALGQKEGARLLAGGKARTDKGGGFFIEPTCFVDVRNDMRIAQEE
                     IFGPVLVVIPYEDDEDAIRIANDSDYALGGCVISASKERATRIARRMRAGAISVNGGL
                     SITGDLPFGGFKCSGVGREWGREGIEEFLDSKVIATRA"
     gene            complement(10270..11919)
                     /locus_tag="PLW13_04170"
     CDS             complement(10270..11919)
                     /locus_tag="PLW13_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69597.1"
                     /translation="MNETFDFVVVGSGGGSMCAALALRAAGKSVLILEKTDLVGGTTA
                     ISGGVMWIPDNRFMKSEGIDDSPAQAIAYLDAVVGDHYDTPGASRERRRAYVEQAPQM
                     IDFLVSQGIRLRRIPSWPDYYAAPGESVPGRTVVSELFDINRLGEWKSRLRPGFLPLA
                     ANLDEAMELANFKRSWGSKKVIARVIGRTIASRLAGKHLATAGQALQGQMLHAALKAR
                     VEIRVNAGARQLVVEDGRVTGVVLETNGAEWRIGARSGVLINAGGFARNQRMLDRYIP
                     GTSTEWSNTARGDTGEMIEEGLRIGAAVAQMDERIGSPMALPPGNPPVKPGMQGDISK
                     PHTIVVDQSGERYMRESSSYMEIARAMRARDTKVPAVPSWMVLDSQYLGKYMLAGTMP
                     GARKPKQWFEEQFLRRGDTLEALASACNMDPARLRDSVERFNSFVRKGRDEDFHRGDH
                     AYDQWLGDSLHRPSRTLGTIEQGPFFALALYPGDVSTYGGLVTDVHARVLREDGSVIP
                     GLYATGTSTASVMGRASPGAGGSIGPSFTWGYVAARHAIQA"
     gene            complement(11939..12676)
                     /locus_tag="PLW13_04175"
     CDS             complement(11939..12676)
                     /locus_tag="PLW13_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69598.1"
                     /translation="MSKIIVITGAGDGLGRALARRFAADGETVVLLGRTLAKVEAVAA
                     AIGERAMAVCCDVSSPDSVRAAFAQIAARFPRIDVLINNAAVYEPIPIAEASDQQILG
                     AIGTNLTGPILCARAAIPMMGRGSHIINVSSESVEMRFPFFVLYQGSKAGLERFSEGL
                     NHELDPSGIKVTLVRAGQMIEEGKMPAGDPGMRMRFAQAALAAGLDMRALPKSQYSSA
                     TNVFRSIIDLPPDLQTPVVTIHARAAD"
     gene            complement(12749..13513)
                     /locus_tag="PLW13_04180"
     CDS             complement(12749..13513)
                     /locus_tag="PLW13_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69599.1"
                     /translation="MALYTTIHLHNVAAGRDADYAAWFDGTHRAALAQLRGFTSAERY
                     EVTPQQMMPDIPQPWRFLSVYDFDLPDPAIDIPALGPLVADARDAGMIADDETERLYT
                     YRLFADWKASPNWQQSEPFSGVSIILANYVPGRREEYQTYYENVHSVEVINVPGHVAM
                     KRGEFSPIQIEPRRFCPGDQLVMCAQQTDDLPFTLRDFSARAGGRSPSGIAMQPRSSS
                     ASIARTVHYFSKVSGSRFWSGGIAYAGDLSVYPDKG"
     gene            13700..14509
                     /locus_tag="PLW13_04185"
     CDS             13700..14509
                     /locus_tag="PLW13_04185"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: protein motif:HMM:NF009092.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HQY69600.1"
                     /translation="MDFLGYKNKRVIVSGCFSGMGEATARLLVELGAQVHGIDYKETT
                     LDLASFNQVDLRDPAAIDAAIARIGGKVDALFNCAGLPQTAPALDVMKVNYAGTRRVT
                     ERVLPLMSAGGAIASISSTGGLGWSRRMPVLMQLLSIDNYDDAIKWCEEHPEDVKEGY
                     TLSKEAIIVWTMLSSQRFIKQGVRINCTLPSPTQTPMMSHFESATPLSVIDAFTLPTA
                     RRSTPEEQAGALVLLNSAGAGFINGVALPVDGGFMGGLTTGQIDLGKIMSR"
     gene            14579..14983
                     /locus_tag="PLW13_04190"
     CDS             14579..14983
                     /locus_tag="PLW13_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY69601.1"
                     /translation="MAAPRPLPRLDAENRPFWTAGAAGELRIMRCRDCQAFIHPPRPV
                     CRSCLSENVAPEVVAGTGVVDTFTVNHQKWHPAAEVPYVIARVALDGVPGVYLTTNIV
                     GCPVDEVDIGDRVRVRFEQQDDVYLPLFEKVV"
     gene            14986..16149
                     /locus_tag="PLW13_04195"
     CDS             14986..16149
                     /locus_tag="PLW13_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY69602.1"
                     /translation="MAAANEAYITGVGQSQIGIRLTRHPLLLTLDAVREALDEAGLTI
                     GQIDGVSTYPGRTPGYLGFSPVGADELVDALGIRATWYAGGGEITSQLGAVVAAAAAV
                     RAGQARHVICFRTVYEAAAMSRPEEYPVPRRDRVDGTSQWVAPFNATSAVNWTAQYAM
                     RHVKKYGLTREQLAQIALTDRAHALLNPRALVREPLTMDEYMSARMISSPLCLYDCDR
                     FSDASTVLIVSAGDAVDEVSCKPVRIAAMAGTVERLSWDQAEWMACYPTGADLWKRTD
                     YKPSDVDTAQLYDGFSFQAVAWLESLGFCDVGEGGRFIEGGKRIALDGELPLNTFGGQ
                     LGAGRLHGFGFAHEAVVQLRGLGGARQIPGDPRVAVCASGGGPLATALLLARD"
     gene            16250..16888
                     /locus_tag="PLW13_04200"
     CDS             16250..16888
                     /locus_tag="PLW13_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HQY69603.1"
                     /translation="MEDDYHCMSVIVHHDGAIATNVEAVMRRAPWTTCPGAPQQLAQT
                     FCGVALDAFAARGEKQVNCTHLHDLAVLAAGHAFDAEPLVYDIFVSDPVDGERRAELR
                     CAGATILGWTEAGMRIVEPAELAGLKLDELRPWIASLDPRRQEAARLLRWANTLANGR
                     MIPLEQQSDASRMPPGCYTFQPHRAVQARRVGVIRDFSDGMAQPLGEYEPAR"
     gene            16960..18285
                     /locus_tag="PLW13_04205"
     CDS             16960..18285
                     /locus_tag="PLW13_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69604.1"
                     /translation="MDMNDMVIISVDDHISEPPEMFDRHLSGEDLATAPKLRTNADGT
                     NYWEYQGMIMPSVGLNAVVGRPLEEYGMEPSSFEQLRKGVYDVHARIDDMNVNGIAAS
                     LNFGTCVGFDGGRFHKAADKGKARIHMRAYNDWHIDEWCGAYPGRFIPCALLPTWDMK
                     ATVEEIGRIAKKGCTTVSLNENPTTQGLPSIHNEYWEPMWKAITDNNMTICLHIGAGN
                     PVPHASQETPIEAWITTMPLSSCVGAADWLSLSALQRYPDLKIALSECGLGWIPYFME
                     RADFSHARHKAWTHSDMNFKGQKPSDIFRKHFMVCFIDDAFGLQNLGAVNEDMVSYEC
                     DYPHSDTLWPEVPERLWETIRHLSPVQIDKITHRNAMRHFSFDMFRHHKREELTVGAL
                     RARAAAAGVDTTPKSAGGAKPLAEGEALRRVTSGDLFKMFAHHANTAAD"
     gene            18375..18665
                     /locus_tag="PLW13_04210"
     CDS             18375..18665
                     /locus_tag="PLW13_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQY69605.1"
                     /translation="MPAYVIFTREGAIRDESAMETYARMNRESPPDPKLTPLVVYGAL
                     ESLEGDAPDGVVILRFPTVEDARAWYNSPAYQAAIVHRKKGADYRAFIVEGL"
     gene            18707..19381
                     /locus_tag="PLW13_04215"
     CDS             18707..19381
                     /locus_tag="PLW13_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69606.1"
                     /translation="MEPASPERAKARRRRDPAATREAILAAAMTVLASDGRDALSLTK
                     VAQLAGVNRGTAYQHFNTREDLVKATVAWVSERLADTVFGAPDDSGLVPDATQRPVYE
                     AITGLVDFAVENPGLGRIWLFEVLASENPGEDVFFKRFERSTRELADSEFGAEGIDAE
                     ALSVMMLGGYFLWPLWVRAGARTKKEREAMAQRMRREVLRLSLHGILRAERFPHLQKL
                     LDELGP"
     gene            complement(19396..20172)
                     /locus_tag="PLW13_04220"
     CDS             complement(19396..20172)
                     /locus_tag="PLW13_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQY69607.1"
                     /translation="MKDSKAPAISRAAAVLRLLGKSDTPLGLQTIARELGLVPSTCLY
                     VLRALVAEEFVSFDPDTKRYSLEAGVLTLARQWLRRNQFADLAQPVLERIGRDFDVTM
                     LGVQIVGLDHIIVVAVSQSGSNFQLSAQIGSRFPALISATGRCIAAFEDHPEAELHAR
                     FSTLRWDEPPTFDEWKAQVSQTRAQGFGVDEGNYISGVTVVAAPVWKTRAKLSHALVA
                     TSIGSALKRAGLPALQRALLSAAQTLSNQLCGDDAAAWRP"
     gene            20434..21999
                     /locus_tag="PLW13_04225"
     CDS             20434..21999
                     /locus_tag="PLW13_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69608.1"
                     /translation="MARLTELTRYADAQAHANSQALWELFDGDREHLNIAHECIGRHA
                     DGSGRAAVRIAHADGADEFLSFDDIAAGSARFAHWLDANGIQPGDRIAFMLEPSLPFY
                     VCLFGAMQTGAISVPLFTLFGLDGLRLRVDDCKPKLLITNAEKAEVAGKIEGVRVIVA
                     DAGLLEEIARFPGSYETKTRADDLAVFQYTSGTTRELPEAVKHTHRALVTLMFAALYG
                     TGVRPGDEFFCPSSPAWGHGLWHGTLAPLALGVTTGTFAGRFDATRLMKALQDYRITN
                     MSAAATHYRMMNNSGRAGDFTFSIRKLSYTGEPIDPVTLEFIDNTFHVPACSMYGTTE
                     IGVVLVNYPGASDFAVKPGSLGKPVPGLKLQVQGPDGTPTAPGVVGELMLWRRDRWET
                     TKDLARIDEDGYFYHAGRADDVIISAGWTMSAVEIENTLLKHPDVREAAVIGVPDATR
                     GQVVKSFIVTDRPQSAEFVDELKNFTRERLSQHEFPRHIEFVSELPKTPAGKIHRKVL
                     RDREAAAAAASAN"
     gene            22018..>22948
                     /locus_tag="PLW13_04230"
     CDS             22018..>22948
                     /locus_tag="PLW13_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HQY69609.1"
                     /translation="MSTSTNKFPKITEQGLDDLRKRIGVKITDTLEPWNYEATRDAIR
                     HYAHGIGDDNPLWCDPEYAAKTRYGTIVALPSFMFTTSRIISGYCGGLSGVHAMWAGA
                     DWTWHKPVLRNDVISTDAWLKDLVEHQTKFAGRSFQQIYHVDFFNQHGDKVAEGDSWV
                     FRTDRDEARERGTKYTEARGRVERYTEEQLAEFTRLYQTEEIRGATPRYWEDVKEGEA
                     LPRMMKGPMTVTGFICYAQGWGGLYIRANKLANKMQQAHPGLGIRNRFNVPDCPERVH
                     WDEEFALEVGAPGAYDYGPERCSWLTHHVTNWMG"
BASE COUNT         3862 a         7291 c         7633 g         4062 t
ORIGIN      
        1 ggcgtcgcgg gcgccgcgat tcacatcgag ggcaggactc gcgcgcagtg gtgcgacaac
       61 gcgcagcgct acggggaaga cgtgctgtcg aagctcgatg gctgaaagcg ctttggccgc
      121 tttggcttcg atctgcccgc gcatcgatca gccagccgga attgttgcag cccatgcctg
      181 cggccgagac ccgagccgct cctgcagggc aagtggcgcg acctgcacct tggtttcgtt
      241 gttgggaccg cgcagctgct cgaacacggt cggcaactca tccagcgtga ccaccgagct
      301 gatcagcatc ttcgggtcga catggccggc gtccatcacg tcggccacgt agtgaaagtc
      361 cttcagcgca tagccgaccg gaaactgcag cgacacgccc ttgtagcctg cgacggccgg
      421 gaccacctgg tcgggtgagg tgcagaatcc catcgacacc accttgccga gcgtgcgcgc
      481 gtgctggatg cccttcatca ggaatcccgg tgtgccgaca cattcgaaca cgatgtcggg
      541 ctgcccgccg agggctgcga tgatgccgtc gctctcgttc tcgccgcagt gcacgaaggc
      601 atccgcaccc atttccagcg ccagcgcctg tcgccgtgcc gatcgtgacg tcgcgacgat
      661 gcgcccggca ccgagtcgcc gcgcccagta gatggtgcac agcgcgaccg caccggcgcc
      721 catgaccagc acgcgatcgc cgggctggac acaggacatg cgcacgccgt acaagccgac
      781 cgcgagcggc tcgatcagcg cgccatcggc cgccgagagc gtggcgggca gcttgatggc
      841 gacgctggca gggatgcgca tcatttcggc atagccgccc atgacgcccg gcgcgttgtg
      901 gcacagcgcc aggttgccgt gatggcaggc ctcgcagttc ccgcagccgg tcgagggcag
      961 ggcggtgatc agatcgcctg tcctgaagcc ggtgacattc gcgccgactt cgacgatttc
     1021 gcccgaatac tcgtgaccgg gcacggtgcc gggcgaaaac tgccactgat gcccctcggt
     1081 catgtgcagg tcggtgccgc agatgccgca gcgatgcacc ttgatgatga catcctcggg
     1141 gcctggaacc ggatccggaa gggtttcgag gacgagtggt ttgcccgctt cgcgatagac
     1201 agcggctttc atttcgggcc tccagcggtg gcaggtccct tcggcgccag gtcgaagcga
     1261 ctggtgtagt cgccgaagcg ctcgcggatt tctgcctcgg tcatgccgaa atcggcgatg
     1321 tcgtagtcat gcgtgccatg ctggcgctcg ggatcgtcgg caatgcgctg gagcatggcc
     1381 gcgcgcgcct cgggcctcag gtcttcgccg atgaagccgt agatgcgttc gaccacggcc
     1441 atcgggtcgc gatgcaaatc ggcgtggacg agatccagca cctgcccggg atgcgcctcg
     1501 cgcacgcgtt gcgccttgcg cagggcgttc gcccatttcg ccacttcgcg cgccagcatg
     1561 ttgtgcgcgc gctgctcgta gcggccttcc tccacgatcg gatgcagcgg catcagcagc
     1621 gagcagagcg atggcaccgc cttggccgga tcgcgatgcg tctggatcac tttcgcgtcg
     1681 gggaatacgg cgaagagcag gtccaggttt tcgatgtgac cggggttctt cagcagccag
     1741 cgcttgtcgg gttcggtgct gccgatcaat tgcaggcagc gatagtagtg acgatacgag
     1801 ctctcctcgc tctggcattg ccaccaggcg tcgtaggttg cggcggacca gccgacggtc
     1861 cacaggttcg aaacgaagcc ctggcgcagc acgaggcagc attcatcgac ttcctcggcg
     1921 gccatgttgt gtgcggctcg cttcttcggg gcagcccgga agcgcgcctc gagcgtggcg
     1981 gcggtccggt ggaaggccgg atagtcggtc caggtctccc ggggcgggcg cggcatgggc
     2041 gaatcgagca gccaggtttg cagcccctgg aagcgctggt cgacggccat gagcttgtgc
     2101 agcgcggtgg tgccggttct cggcatgccg gtgatcacga ccggactcgg gatcgcgtgg
     2161 cgatcgaagc cgggatgttc ggccattgac cggatcgcct gcgcgcggcc cttgagcacg
     2221 ccgacgaccg tgccccacgc gtagtgccgt ccgcgttcgc tgaagcgcgg atcgtagtcc
     2281 atcgactgca gcagcactct caggcccggc aggtagtcgt cgctgccgaa gtcggtgctt
     2341 ccgacttcct ttgcgaccag ttcgtgcagt tggtccagtg ccgtggcgaa gaccccgggt
     2401 gcagcgtatc tgtcatccat cgcggttcac tcgtcgggaa tgccgctcat cgacgggccg
     2461 ccgttgaccg caacagtctg cccggtgatg taggaggcgg catcgctggc gaagaagacg
     2521 atcatgttgg ccacttcgtc cggcgtgccc gggcggcgca gcgggatcgt cgtgagattg
     2581 ctcatgtcga gcttcgccac ttcccacgcc gcggccgcgc gctccgatgc gatcatcccc
     2641 gccgcgacgc agttggcgcg aatgccatgg cggccccatt cggccgccgt gacggtggtg
     2701 aacatctgca gcgccgcctt ggccgcggaa taatgcgccc cgcctttcac gccgctgatg
     2761 ccggcgttgg aggagatatt gacgatcgcg ccggatcgct gcgcgatcat gtgcgggccc
     2821 accgcgtgcg tgcagagata ggcggcgcgc acattgaggt cgaagctggc gtcccaggcc
     2881 ctgctgccca gcccggcgag cggccccagg cgcgagccgc cggcgttgtt gacgaggatg
     2941 tcgatgcgcc cgaattcatc cagcgttcgc tggaccaggc gggtgacctg ttcctcgtcc
     3001 ttgacgtcgg tcggcactgc caggcagcgc gcgcccgatt cctcgcgcac gcgcgcggcc
     3061 gcgcgctcca gttcatcggc ggtgcggctg gcgacgacga cagcggcgcc cctgcgcgcc
     3121 aattgccgcg cggtcgcgag gccgatgccg gtgccgccgc cggtgacgat ggcgacgcgg
     3181 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgcggtc gcgaggccga
     3301 tgccggtgcc gccgccggtg acgatggcga cgcggccggt gaaatcggaa tggtcggggt
     3361 tcatggggtt taccatctcc ttcgtcgttg acacgcgcgc acccgccggc gcagttgctc
     3421 ggctcgttgc tgtggcgtca ccaccggtgt gtcggcaggc aggtggtggc gcaactcggc
     3481 cagcttcacg cgcctgatca ccggtgtcgg gttggtgtcg caatcgaacc agcgcccgta
     3541 gatgccgcct tccctgtatc cggcgggatc gagccagttg ggcacgccgg ggtcttccag
     3601 cgcgatgacg gcgcggaacc tgccgtccga actggctcgt gccgtatggc cattgaggct
     3661 ggaaaggcga tagacgtatt cgagtgcgtt gaaatacggg tcgttgagct ggatgttcca
     3721 ataggggcgg cgctcgggca gatcggtctc gatgatcagc gcctcgtcct cctccagctg
     3781 gaagcaggcg ggccagtaga cctgcttggc gagcgcgccc ttcatgcgca ccggctcgaa
     3841 cacgttgaag ccgacgcgct ctttcacgcc gttctgcagg cggtagtagc tgcgggtctt
     3901 gcgcgcgggg aacttcgcca tttcccggat gcgcgcgatg atctccccgg gcgcgaggcg
     3961 cggcttggcg gggacgggat ccaggcactc gatcgagagc gtggggtcga tctcgttctc
     4021 ccagtcgtag ctgcgcaggc gcaccatcat gccggtggcc tggggatgga tcggtgccca
     4081 gttgccggca tggccagccg ggcgttcgac gctgaaaacc acttcgaatt caccgccggg
     4141 ttcgacaacg atgtcctggt catcgatctc gttgtgcccg ccgatctgcg cgatgtcatc
     4201 gaccatgccc gacatgaagc gctgcgtggt gaagctcagg atcttcaccg tgccgcgatt
     4261 gccgctgacc ctgtatttca ggtcaccgcg tatcgggctg tagagataga tgtcgtcggg
     4321 attgggctgg agcgtgaaca ccgggttcca cagcggagcc cagtcggggt gttccggcgt
     4381 ggcgtggaaa taggcgaagt aggaatagga caggctcatc atcgtctgcc gatagacgtc
     4441 ggcgcgataa tccgcgtgct ccgggtgcca ggtgttcgcg agttccgcga ccgcatcctc
     4501 cagcgcgccg agatattcga ggatgctcgg gccgatacgc gtgtccatgt gcaattcctt
     4561 cgtgctcggc ctgtgaatgt tacgctcagt tgcccggcac gctctcgaaa aaggccagcc
     4621 cggcaggttc ggggaacagg tattcgcagt aatgccccag ctccggggcc tcgacgaagc
     4681 agctctgcat gaagcccgga ttgctgctcc tggagggcat gttccagccc ttgcgtgcga
     4741 tctccgtcag cagggcgttc cactcgtccc cgtttcggat caggaagccg aggtggtgat
     4801 gacgaatggc gaattcgtcg gctggcaatc gcccggtgta cagctgcgaa cccggaccgt
     4861 tcgcggtgag caattcgtac atcgtgccgc cgacccacgc cagttcgacg tggatgtgac
     4921 cgcctgccgg cagctgtcct tccaggcgcc ggaaggcact gatgccgtag cgctccccga
     4981 aaatatcgcg cgcccgctcg atgtcgttgg tcgcgtacgc cacctggaac tgctcggcgc
     5041 gcagcagccc tgcggcaggg tcgaacgtgg catccggtct gttcatacgc tcggcctcat
     5101 cgtgcaggaa ttgcccgccg cccgggcggg agcgggtcgg cgcgccttgc tcgcgccccg
     5161 atcgggagat gtcccgagtg ccccggttgc aatagatagt attcactatc tacaaggtct
     5221 ggcaagatgc tgcctggatt ttcaggcgtg gattgcagca ccaccgcggc cggttggcgt
     5281 ggcgggcagt tcgaggcagt gcaggagtgg agcggggatt ggctacgcgg cgagagacaa
     5341 cggcagctgc agtgactcct ggcgcggtgc gcaggaagaa gcggcgctcg cccggggaga
     5401 tcagggaacg gctgatcgat gcggcccggg aggagttcaa gcgctgcggc ttcgtcgggg
     5461 caacgacggc ggcaatcgct cgcaacgccg acgtcaccga agcacagctg ttccgttgtt
     5521 tcgagtcgaa ggccgcactt tttcgcgagg cggttttcga gcggctgaac cagcattttt
     5581 ccgagttcaa tgcgcgatat ccgaccgatg tttccagtcg ggcgaacgtt cgcgatcgcg
     5641 aacgccggta cataaccgag ttgcagcagt ttctcgacga gcactcgaaa ctgctgctgt
     5701 cgctggttgc agcccagagc ttcacctcgg gcagtctgca gggagtcggt gagatcgaga
     5761 gcctgcgcca ctacttcgag cgcggcgccg cgacgatgag cggcaggatc ggggaggatc
     5821 ccccgctgga tccgaaattg ctggtgaggg tgtcttttgc ggcggtgctc ggttgcgtga
     5881 tcttcaagga ctggatcttt cccgccggtc tcgcgagcga cgacgacatc aatgccgccg
     5941 ttatcgattt cgttatcgat ggaatcagca tcaactcgga tcccgggacc aaagaacctg
     6001 tgcggagatc cggcaagggg agagcaacat gacgccggcc aacactcaac tcgttcgcga
     6061 tttcttcacg gctctcgcca gcggcaatgt gcctgatgat ctgctgacgc cggatatgtc
     6121 cctctggacg gtgaacggag gcacgtcgga cgaggcgcgt tttcagggcg ccatgaaaaa
     6181 ggccgcgggt taggcggggc tggaacacga gcggagccgc gtgatgcaaa ccagggcaaa
     6241 gtccttctgc cgcaattgcg gcgcgctgtg ctcgatggag ctgaccatcg aggacggcaa
     6301 gctggctggc gtgactgctg acggtagcgc ctcgccctac ggggcctaca tgtgccccaa
     6361 gggccgcgcc agcctggatt ttcataacgg ggcggaaggc cggctgctga acagcctgcg
     6421 ccgcgaggcg tccggcgcgt tcagcgagct gccggcggcc caggccctgg acgagattgc
     6481 cgccaggctg gcggccctga tcgcggaaca cggaccgcgc tcggtcgcct tctatcacgg
     6541 caccggcgcg taccgaagcg tgctgggcgc tctgctggag cgagcattcg cctcgagcct
     6601 cgggtcgccg aacttcttct cgacgatgac gatcgaccag tcggcgaagt gggtcacggc
     6661 cggtcgaatg ggcgtgatgg cgagcggcaa gcccagcacc cgggacatcg accttgcggt
     6721 catcgtcggc aacaatccgc tggtcagcca ccagacctac ccgttcgggc ccggggagag
     6781 cggggcaccg ggcaagtcgt tcgccgcggc tcgggcgcgc ggcgcgcgca tcgtcgtggt
     6841 cgatccgcga cgcacggaaa cggcgcgcta cgccgatctg ctgatccagc cacttccggg
     6901 gcacgacgcg gcgctctttg cagcagtcgc ccacatcctg ctgcgcgacg gcacctgcaa
     6961 cacggcgttt tgcgaacgct tcgtcaccca gctggaccgg ctggccgagg ccgtcgcgcc
     7021 gttcacgccg gaactcgtgg ccaggcgcgc cgatgtgccg gtggaacaga tcgagcaact
     7081 cgcgcggtgg ctgggcgagg cagagcgccc gttcgtgggc agcggcagcg ggccctcgat
     7141 gtccgcccat tccaatctca acgaccacat gatcgagacc gtgaacgcgc tggtcggcgg
     7201 ctacaggcgc gccggagacc tggtgcgcaa tcccggcacg ctcaagccgc gcgtgtgcat
     7261 cgagacggtg gtcccgccgc agcgcagctg ggaaaggggc gtgaagtgca ggacggccga
     7321 catcggcgcg ctctacgggg agtttccgac ggcgctgctg ccgcaggaaa tcctggcgcc
     7381 ggggccggac aggattcgcg cgctgatcgt gttcggcggc aatccgctga cggcgctcgg
     7441 cgatcccgca cgggccgagc ccgctttcgc ggatctcgag ctgctggtca gcctcgatgc
     7501 gcgcatgaac gaaacggccc ggatggccca ttacgtgatc gcgacgtcgc agcatttcga
     7561 gcgtcacgat ctcagcattc cgggagacag tctctttccg gaggcctttg cgcaatacgc
     7621 gccaccggtg gttcccgggc ccgccggaac gatccatgac tgggagttct tctggggcgt
     7681 cgcgtccagg ctgaaactgc cgttgacgct caagtactgg aactatggat tgtcctatga
     7741 cgcgatcccg ggcggactgc cgctggacat ggagcgcgcg ccccatcccg aggacatgat
     7801 gcgttatctc tgcgagcacg gtgacgtgtc gttcgaggag ttgcgcgcga atccgggcgg
     7861 cgtgcggccc gtccgcccgc cgcagtacgt gaaggcggcg ccggacaacg gtgcgcggct
     7921 cgagctctgc ccgccggacg tcgcggccga attgcatgcc gtgctcgagg aagtggtgga
     7981 cgatcggttc aagtacagac tgacctgtcg ccggatcctg gatgcgatga acagcgcgta
     8041 ccgggactcg ccgcaaacga tcaggaaata tccgctgaac tgggcttaca tgaaccccga
     8101 cgatatgcgc gatgacggta tcgacgacaa cgaactgatc gagatccagt cggaatcagg
     8161 tcgtatcctg ggtgtcgcca gggccgagag ccagctgcgt cggggcgtgg tgtcgatgac
     8221 gcacctctac ggatctctcg ccccttcgac cgatccctac gctcaacgcg gaagccacac
     8281 ggggcggctg acttcgctgc agaagcacct ggagcccatc aatttcatgc cgcgcttttc
     8341 cggcattccc gtcaatattc gccgggcggg cggcgatgtc ccggcaactg ccccgttggc
     8401 gacgatatta atcagcccag tgcgtcggcg tctgccacca ccgggcgact ctcgcgcgtt
     8461 gcgcgcaaac acctgtcggg ctcgccgcag tcggcgatct cgatgtaatc ctcgtgcacc
     8521 gccctgatca ccgccgcgaa cgggaaatca cccagcgagg gcacgcgata gctcacggtg
     8581 tcgcccacct tgaaggtcga gggatcgaag ttgaattcaa acggacattt gccgtccatt
     8641 ccaatcacga tgcgtactcc ccgcgcaata gtctgcgcat gcaatggtcg aacaccgcca
     8701 cggtggctgt cgctcgctag gccctggtgg cgatgacctt gctgtctaga aactcctcga
     8761 taccctcgcg gccccactcg cgaccgacgc cgctgcactt gaatccgccg aatggcaggt
     8821 cgccggtgat gctgaggccg ccgttgacgc tgatggcgcc ggcgcgcatg cgccgggcga
     8881 tgcgcgtggc ccgctccttg ctggcggaga tgacgcaacc gcccagggcg taatcgctgt
     8941 cgttggcgat gcggatcgcg tcctcgtcgt cctcgtaggg gatcaccacc agtacgggac
     9001 cgaagatctc ttcctgcgcg atgcgcatat cgttgcgcac gtcgacgaag caggtcggct
     9061 cgatgaagaa tccgccgccc ttgtcggtcc gcgccttgcc gccggcgagc aggcgcgcgc
     9121 cttccttctg ccccagcgcg atgtattcca tcacccggtc gcgctggcgc gccgagatga
     9181 ccgggcccat gacctgggtg ggatcgtcga aatcgcccca cttggtggcg aagcccgcat
     9241 aggccatctc cagcgccttc accgcctcgt cgtaacggct gcgcgggacc agcaggcggg
     9301 tagggatcgc gcagccctgg ccggcatgga aaatcaccat cgactgcatc acggacatcg
     9361 catagtccgg ggcgtcgtcg aggatgatgt tcgccgactt gccgccgagt tccaggaaca
     9421 cgcgcttgag cgtcgccgcg gcggtttcca tgatccgctt gccgacgccg gtggagcccg
     9481 tgaacgagat cacgtcgacc ctggggtcct tcaccagcgc ttcgcccgcc atgacgggat
     9541 cggcggacgt gatgacgttg aacacgccgg gggggaagcc ggcttccacg gcgagctcac
     9601 ccatgaacgc ggcggtcgcc ggggtatccg gggcaggctt cagcaccacg gtgcagccgg
     9661 caagaagcgc gggcacgacc ttgccgacat tcacgtacag cggcacgttc cagggcgaga
     9721 tggcgccgac cacgccgagc ggctcgtgaa ccacgatccg gtcgctgtcg aagcccattt
     9781 cattgcgccg cccgcgatcc tcttcccacg tgaccgacgg gaagcaggcc agcacgtcgt
     9841 ccatgccgct gagcgcccgg tcgacctgcg cgcggtaggc agctccgatg gaggagcccg
     9901 cctcgaggcg gacaatctgg accagccggt cgcggttggc gaacagcagg tcgcggtatt
     9961 tcttcatcag cgcgaagcgc tcttcgtgcc gctgcgacca gtcggtctgg tcgaaggcgc
    10021 gccgtgccgc ggcgatcgca tcgttcacgt cgtccgcgga ggcgtccgcc gacttgccga
    10081 tgacctcgcc ggtccacggg cagatattgt caaaggtcct gttgccggcc gccgcgcgca
    10141 gcttgccgtc gatatagagc ctggcttccg ggagcatttc catgtgtctg tcctttctgg
    10201 gtttcctggg gcagcgccgt caggcgctga acgggttgaa ctgtcgcggc attcccgtgg
    10261 ggacagccgt catgcctgga tcgcatgcct ggcggccaca tagccccagg tgaacgacgg
    10321 gccgatactt ccgccggctc cggggctggc ccgtcccatc accgacgccg tggaggtgcc
    10381 cgtggcgtaa agcccgggta tcaccgagcc gtcctcgcgc agcacccgcg cgtgcacatc
    10441 ggtcaccagg ccgccgtagg tgctgacatc gcccggatac agggccagcg cgaaaaaggg
    10501 gccctgctcg atggtgccga gcgtgcgtga gggccggtgc agcgagtcgc ccagccattg
    10561 gtcatacgca tggtcgccac gatggaagtc ttcatcgcgg cccttgcgga cgaagctgtt
    10621 gaaacgctcg accgaatcac gcagcctcgc gggatccatg ttgcaggcgc tcgcgagcgc
    10681 ttcgagcgta tcaccgcggc gcagaaactg ctcctcgaac cactgtttcg gcttcctggc
    10741 gccgggcatg gtcccggcca gcatgtattt gcccaggtac tggctgtcga gcaccatcca
    10801 gctcggcacg gccggcacct tcgtgtcgcg cgcgcgcatc gccctggcga tttccatgta
    10861 ggagctggac tcgcgcatgt agcgctcgcc gctctggtcc acgacgatgg tgtgaggctt
    10921 ggagatgtcg ccctgcatcc cgggtttgac gggcgggttt cccggaggca gagccatcgg
    10981 cgagccgatg cgctcgtcca tctgtgcgac ggcggcaccg atgcgcagcc cttcctcgat
    11041 catctccccg gtatccccgc gcgcggtgtt ggaccactcg gtcgatgtgc cggggatgta
    11101 gcggtccagc atgcgctggt tacgggcgaa gccgccggcg ttgatgagca cgccggagcg
    11161 agcgccgatg cgccactcgg caccgttggt ctccagcacc accccggtca cgcgtccgtc
    11221 ctcgaccacg agttgcctgg cgcccgcgtt gacgcgaatc tccacgcggg ccttcagcgc
    11281 ggcatgcagc atctgcccct gcagtgcctg ccctgccgtg gccaggtgct tgcctgccag
    11341 ccggctcgcg atggtacgcc cgatgacccg ggcgatgacc ttcttgctgc cccaggagcg
    11401 cttgaaattc gccagctcca tcgcctcgtc gaggttggcc gccagcggaa gaaagcccgg
    11461 gcgcagtctg gatttccatt cgcccagccg gttgatgtcg aacagttcgg aaaccacggt
    11521 gcggcccggc accgattcgc cgggagcggc atagtagtcg ggccaggagg gaatgcgccg
    11581 cagacgaatc ccctggctga ccaggaagtc gatcatctgc ggcgcctgct cgacataggc
    11641 ccgtcgccgc tcgcggctgg caccgggcgt gtcgtagtga tcgccgacca ctgcatcgag
    11701 ataggcgatg gcttgcgcgg ggctgtcgtc gatcccctcg ctcttcatga agcggttgtc
    11761 ggggatccac atcacgcccc cggaaatggc cgtggttccc ccgaccaggt cggtcttctc
    11821 caggatgagc acgctctttc cggccgcgcg cagggccagc gccgcgcaca tcgagccgcc
    11881 gccgctgccg accaccacga aatcgaatgt ctcgttcatg tcactgaact cctggtcgtc
    11941 agtcggccgc gcgggcgtgg atggtcacga ccggggtctg aagatccggc ggcaggtcga
    12001 tgatcgagcg gaatacattg gtcgccgaac tgtactggga cttcggcaac gcgcgcatgt
    12061 cgagtcccgc ggccagggcg gcctgggcaa aacgcatcct catcccggga tccccagcgg
    12121 gcattttccc ttcctcgatc atctggcccg cgcgcacgag ggtgaccttg atgccgcttg
    12181 gatccagttc gtgattgagg ccttcggaga agcgctccag ccccgccttg ctgccctggt
    12241 agagcacgaa gaaggggaag cgcatttcca ccgattcgct gctcacgttg atgatgtggc
    12301 tgccccggcc catcatcgga atcgcggcgc gcgcgcacag gatcggtccc gtcaggttgg
    12361 tgccgatggc gccgagaatc tgctgatccg atgcttcggc gatcgggatc ggttcataca
    12421 cggcggcgtt gttgatcagc acgtcgatgc gcggaaagcg cgcggcgatc tgcgcgaatg
    12481 ccgcccgcac cgagtccggc gacgacacat cgcagcacac cgccatggcg cgttcgccga
    12541 tcgccgcagc cacggcctcg accttggcca gggtgcgccc gagcagcacg acggtttccc
    12601 cgtcggcagc gaaacgccgt gcgagcgcgc ggccaagccc gtccccggcg ccggtgatga
    12661 cgatgatctt cgacacgggt ggtctccttg tggttgaagg gcggtcggcc gactgatttg
    12721 ccagggtcgg tggcggacgg ggagcgcctc agcccttgtc cgggtatacc gaaagatcgc
    12781 cggcataggc aatgccgccg gaccagaagc ggctgccgct gaccttgctg aagtaatgaa
    12841 cggtccgcgc gatggaggcg gacgacgagc gcggttgcat cgcgatgccg ctggggctcc
    12901 tgccgccggc gcgtgcgctg aaatccctga gggtgaacgg caggtcgtcg gtttgctgtg
    12961 cgcacatcac gagctggtcg ccggggcaga aacgccgcgg ttcgatctgg atcggggaga
    13021 actcgccgcg tttcatggcg acatggcccg ggacgttgat cacttccacg ctgtgcacgt
    13081 tttcgtagta ggtctggtat tcctcgcgtc ggccgggaac atagttcgcc aggatgatgc
    13141 tgacgcccga aaagggctcg gattgctgcc agttcgggct ggccttccag tcggcgaaga
    13201 ggcgataggt gtagagccgc tcggtttcgt cgtcggcgat catgcccgcg tcgcgcgcat
    13261 cggcgaccag cggtccgagt gccgggatgt cgatggccgg atcgggcaga tcgaagtcgt
    13321 agacgctcag gaagcgccag ggttgcggaa tgtccggcat catctgctgc ggcgtcactt
    13381 cgtagcgctc cgcgctggtg aagccgcgca attgcgccag ggccgcgcgg tgcgttccgt
    13441 cgaaccacgc cgcgtaatcg gcgtcgcgtc ccgcggcaac gttgtgcaga tggattgtgg
    13501 tgtaaagcgc catatcttat tgtccagcct ggatttctgg tccacggttt cgacgggcca
    13561 ggccgcgcga tctgcggcgg ccgacgagcc ggcccgcgct gcggcaagtt ataatcacca
    13621 ctgttgacta acacgtcaag tggcgtatgc tttcggccca ttccgcagcc ggttgcatca
    13681 attcatcgag aggttggcaa tggacttcct gggttacaag aacaagcgtg tcatcgtcag
    13741 cggctgcttc tcgggaatgg gtgaggcgac tgcccgcctg ctggtcgagc tcggcgcgca
    13801 agtgcacggt atcgattaca aggaaacgac gctcgacctc gcctcgttca accaggtcga
    13861 tttgcgcgac cctgcggcca tcgatgccgc gatcgcgagg atcggcggca aggtggatgc
    13921 gctgttcaat tgcgccggtc tgccgcaaac cgcccctgcg ctcgacgtca tgaaggtcaa
    13981 ctatgcgggg acccggcgcg ttaccgagcg cgtcctgccg ctgatgtccg ccggtggcgc
    14041 gattgccagt atttcctcca ccggtggtct cgggtggagt cgccgcatgc ccgtgctgat
    14101 gcagttgctc tcgatcgaca actatgacga tgccatcaag tggtgcgagg agcaccccga
    14161 ggacgtcaag gagggctaca ccctgtcgaa ggaggcgatc atcgtgtgga cgatgctgtc
    14221 gtcgcagcgt ttcatcaagc agggtgtgcg catcaactgc acgctgccca gtcccacgca
    14281 gacgccgatg atgagtcact tcgaatccgc cactccgctg tcggtgatcg atgccttcac
    14341 gctgcccacc gcccgtcgct cgacccccga agagcaggcc ggtgcactgg ttctgctcaa
    14401 cagcgccggg gcgggcttca tcaacggcgt ggcattgccg gtcgatggcg gattcatggg
    14461 cggcctgacg accgggcaga tcgatctggg caagatcatg tcgagatgag cgccgcatcc
    14521 gcggcgctcg tgtgctcgga ccttgaacac ttcagcttgc aggggataag taacaccgat
    14581 ggctgcacct cgtcccttgc cgcggcttga tgccgagaac cgccctttct ggaccgcagg
    14641 ggcagcgggt gaactgcgca tcatgcgttg ccgggactgc caggcgttca tccatccacc
    14701 gcgccccgta tgccgcagct gcctgtcgga gaacgtggcg ccggaggtgg tcgccggcac
    14761 cggtgtcgtg gatacgttca ccgtcaacca ccagaagtgg catccggccg cggaagtccc
    14821 gtacgtgata gcccgggtgg cgctggacgg ggtgccgggt gtctacctga ccaccaatat
    14881 cgtggggtgc ccggtggatg aagtcgacat cggcgaccgc gtgcgcgtga ggttcgagca
    14941 gcaggacgac gtctacctgc cgcttttcga gaaggtggtc tgaacatggc tgccgcaaac
    15001 gaagcctata tcaccggggt cgggcaatcg cagatcggca tcaggctgac gcgccatccg
    15061 ttgttgttga cgctcgatgc ggttcgcgag gcgctcgacg aggcgggtct gacgatcggg
    15121 cagatcgacg gcgtgtccac ctacccggga cgcacgccgg ggtacctcgg tttctcgccg
    15181 gtcggtgccg acgagctggt cgatgcgctg gggattcgcg cgacctggta cgccggcggt
    15241 ggtgaaatca cctcccagct gggcgccgtg gtggccgctg ccgccgctgt gcgcgccggc
    15301 caggcgcggc acgtcatctg ctttcgtacc gtctacgaag ccgcagcaat gtccaggccc
    15361 gaggagtacc cggttccccg gcgcgaccgg gtcgatggta cttcgcagtg ggtcgccccg
    15421 ttcaacgcca cgtcggcggt caactggaca gcgcagtacg cgatgcgcca tgtgaagaaa
    15481 tatggtctca cacgcgagca gctggcgcag atcgcgctga ccgatcgagc ccatgccctg
    15541 ctcaatccgc gtgcgctcgt gcgcgagccg ctgacgatgg acgagtacat gtcggcgcgc
    15601 atgatctcct ccccgctgtg tctctatgat tgcgatcgtt tttccgacgc ctcgactgtg
    15661 ctgatcgtgt cggcgggtga tgccgtcgac gaggtttcgt gcaagcccgt gcgcatcgcc
    15721 gccatggccg gcacggtaga gcgcctcagc tgggaccagg cggagtggat ggcctgctac
    15781 cccacgggcg ccgatctctg gaagcgcacg gattacaagc cctcggacgt cgatacggcg
    15841 cagctctatg acggcttcag tttccaggct gttgcctggc tggagtccct tggcttctgt
    15901 gatgtcggcg agggcggacg cttcatcgaa ggtggcaagc ggattgccct tgacggcgag
    15961 ctgccgctca acaccttcgg ggggcaactc ggcgccggtc gtctgcacgg ctttggcttt
    16021 gcccacgaag cggtggtgca attgcggggc ctgggtggcg cgcgccagat tcccggtgat
    16081 ccgcgtgttg ctgtctgtgc ctcgggcggc gggcctctcg ccacggcctt gctgctggcg
    16141 cgggactgag gaggtgcgcc cggctccccc ggttcacgtc cccgggcagt tgccgggttt
    16201 caggcggcgt ttccgtgtaa cgccggcggc gggtcgcgta caggcagagg tggaagacga
    16261 ttatcactgc atgagcgtga tcgttcacca cgatggcgca atcgccacca acgtcgaggc
    16321 cgtgatgaga cgcgccccct ggaccacctg tcccggcgcg ccgcagcagc tggcgcagac
    16381 attctgcggc gtcgcgctgg atgcgttcgc cgcgcgcggt gaaaagcagg tcaattgcac
    16441 ccatctgcac gatctcgccg tgctggcggc ggggcacgcc ttcgacgcgg agccgctggt
    16501 ctatgacatt ttcgtatccg accccgtcga tggcgaacgc cgcgccgagc tgcgttgcgc
    16561 cggcgcgacg attctcggct ggaccgaggc cggcatgcgc atcgtcgagc ccgcggagtt
    16621 ggccggtctg aaactcgacg agctgcggcc atggatcgcg tcgctggatc cgcggcgcca
    16681 ggaggcggcg cggctgctgc gctgggccaa tacgctcgca aacgggcgta tgattccgct
    16741 ggagcagcag tccgacgcga gtcgcatgcc acccggttgt tacaccttcc agccgcaccg
    16801 cgccgtgcag gcacggcgtg tcggcgtgat acgggacttc agtgacggga tggcgcagcc
    16861 gctgggtgaa tacgagcccg cccggtgaac ggccgggccc ttgacagcgt ttcgagcccg
    16921 ataatgatca gcatcgcata gcagcgtgag gaggatttca tggacatgaa cgacatggtg
    16981 atcatcagcg tggatgatca catcagcgaa cctccggaga tgttcgacag gcatctctcg
    17041 ggcgaggatc tcgccacggc gcccaagctg cgcaccaacg ccgacggcac gaactactgg
    17101 gagtaccagg gcatgatcat gccctcggtg ggtctcaatg cggtcgttgg ccgaccgctc
    17161 gaggaatacg gcatggagcc gagctcgttc gagcagctgc gcaagggcgt ctacgatgtg
    17221 cacgcgcgca tcgacgacat gaacgtcaac ggcattgccg catcgctcaa tttcggcacc
    17281 tgcgtcggat tcgacggcgg ccggttccac aaggccgccg acaagggcaa ggcacgcatc
    17341 cacatgcgcg cctacaacga ctggcacatc gatgaatggt gcggcgcata tcccgggcgc
    17401 ttcattccct gcgccttgct gccgacctgg gacatgaagg ccacggtcga ggagatcggc
    17461 cgcattgcga agaagggctg taccaccgtt tcgctcaacg agaaccccac gacacagggt
    17521 ctgcccagca tccacaacga gtactgggag ccgatgtgga aggccatcac cgacaacaac
    17581 atgacgatct gcctgcacat cggcgccggc aacccggtgc cgcatgcctc ccaggaaacg
    17641 ccgatcgagg cctggatcac cacgatgccg ctgtcgagct gcgtcggcgc ggcggactgg
    17701 ctcagcctct cggcgctgca gcgctatccg gacctgaaga tcgcgttgtc cgaatgcggt
    17761 ctcggctgga ttccgtattt catggagcgc gcggatttca gtcatgcgcg gcacaaggcc
    17821 tggacccact cggacatgaa tttcaaaggc cagaagccga gcgacatctt ccgcaagcat
    17881 ttcatggtct gcttcatcga cgatgcgttc gggctccaga acctcggcgc ggtcaatgaa
    17941 gacatggtct cctatgaatg cgactacccg cattccgaca ccctttggcc ggaagtcccg
    18001 gaaaggctgt gggagacgat caggcacctg agccccgtgc agatcgacaa gatcacgcac
    18061 cgcaacgcca tgcgccactt cagcttcgac atgttcaggc accacaagcg cgaggaactg
    18121 accgttggcg cattgagggc cagggccgcc gcggccggtg tggataccac gccgaaatcc
    18181 gccggcggcg ccaagccgct ggccgagggt gaagcgctgc gccgtgtcac ctcgggcgac
    18241 ctgttcaaga tgtttgctca ccacgccaat acggcggctg actgagccga ggactcacgc
    18301 gggagtgcaa gctcccgcgg ccggcttcgt gttgcgaatt cgcgcagatc atccatgagc
    18361 aagagacaga agacatgccc gcctacgtca tcttcacgcg tgaaggcgcg atccgcgacg
    18421 agtccgcgat ggaaacctac gcgcgcatga acagggaaag tccgcccgat ccgaagctga
    18481 cgccactcgt ggtttacggg gcattggagt ccctggaagg tgatgcgccg gacggggtcg
    18541 tgatactgcg gttcccgacc gtggaggatg ccagggcctg gtacaacagc cccgcctatc
    18601 aggcggccat cgtgcaccgg aagaaaggcg cggattaccg ggccttcatc gtcgagggtc
    18661 tctgagcggt ggttaggacc agggttcagt ggcagcggtg aacaatatgg agcctgcgtc
    18721 cccggagcgc gcaaaggcgc ggcggcggcg cgatcccgcg gccactcgcg aagccatact
    18781 tgccgcggcc atgaccgtgc tggcatccga tggtcgcgat gccctgagcc tgaccaaggt
    18841 ggcgcagctg gcgggcgtca accgtggtac ggcttaccag catttcaata cccgggagga
    18901 tctcgtcaag gccacggtgg cctgggtgag cgagcgcctg gcggataccg tgtttggcgc
    18961 gccggatgat agcggcctcg tgccggatgc gacgcagcgc ccggtctacg aggcgattac
    19021 cggcctggtg gacttcgccg tcgagaaccc gggcctgggg cgcatctggc tgttcgaggt
    19081 gctcgcctcg gaaaatcccg gcgaggatgt tttcttcaag cgattcgagc gctcgaccag
    19141 ggagcttgcc gacagcgagt tcggggccga gggtatcgac gccgaagcac tgtcggtgat
    19201 gatgctgggc gggtatttcc tgtggccgct ctgggtgcgt gccggtgccc gcacgaagaa
    19261 ggagcgcgag gccatggcgc agcgcatgcg ccgcgaggtg ctgcggctct ccctgcacgg
    19321 aatactgcgc gcggagagat ttccgcacct gcagaagctg ctggacgaac tggggccctg
    19381 aggcagaatt catccctacg ggcgccacgc ggctgcatcg tcgccgcaga gctggttcga
    19441 cagggtttgc gcggcggaca gcagggcgcg ttgcagggcg ggaagaccgg ctcgtttcaa
    19501 tgcgcttccg atgctcgtgg cgacgagtgc gtggctcagt ttcgcgcgtg tcttccagac
    19561 cggggctgcg accactgtca ccccggaaat gtagtttccc tcgtcgacgc cgaacccctg
    19621 cgcgcgcgtc tggctgacct gcgccttcca ttcatcgaat gtcggcggct catcccagcg
    19681 cagcgtgctg aacctggcgt gcagttcggc ttccgggtga tcttcgaacg ccgcgatgca
    19741 ccggcccgtc gcgctgatca gcgcaggaaa gcgactgccg atctgcgcgc tgagctggaa
    19801 gttgctcccc gactgtgaga ctgcgacgac gatgatgtgg tcgaggccga ctatctggac
    19861 tccgagcatc gtgacatcga aatcccggcc gatgcgctcc agcacgggct gcgcgagatc
    19921 ggcaaactgg ttgcgcctca gccattgccg cgcgagggtg agaacgcccg cctccaggga
    19981 ataccgcttg gtatcgggat caaaggagac aaattcctcg gcgaccaggg cccggagcac
    20041 gtagaggcag gtgctcggca ccaggcccag ttcccgcgcg atggtctgca gtccgagcgg
    20101 cgtgtcgctc ttcccgagca agcgcagaac cgcggccgcg cgcgagatgg cgggtgcctt
    20161 gctgtccttc aaggtcctgg cgagttcccg ctccggcgac agaggacgtt tcggttcggc
    20221 atacattgag cgtacccgtt gctgttcgat gatgactgac cgccccatgc ggcgacgtgc
    20281 aagccttggc gccagggaca aggtcctcgg ctgttgttca atatatagaa tcacattcta
    20341 tatttacaac attatttttt tgtggcatct tgcgtccaga tcgaacggcc acgcgaatca
    20401 gcggtggttt cgaggacaag gagtggtgga tccatggcca ggctcacaga actcactcgc
    20461 tacgcggatg cccaggcgca tgccaattca caggcgctgt gggaactctt cgacggtgat
    20521 cgcgaacacc tgaacattgc ccacgaatgc atcggccgtc acgccgatgg ctccggccgc
    20581 gccgcggtgc gcattgccca tgccgacggg gccgacgagt tcctgagctt cgacgacatc
    20641 gccgccggct ccgcgcgctt cgcgcattgg ctggacgcga acggcatcca gccgggtgat
    20701 cgcattgcct tcatgctgga gccgtcgctg cctttctatg tctgcctgtt cggcgcgatg
    20761 cagacggggg cgatcagcgt gccgctgttc acgctcttcg ggctcgacgg tttgcggctg
    20821 cgtgtcgacg actgcaagcc gaagctgctg atcacgaacg cggaaaaggc ggaagtcgcc
    20881 ggcaagatcg agggtgtgcg cgtcatcgtg gccgatgccg gactgctcga agagatcgcg
    20941 cgctttccgg gcagctatga aacgaagacc cgtgccgatg acctggcggt gttccagtac
    21001 acgtccggca cgacccgtga attgccggaa gcggtaaagc acacgcatcg cgcgctggtg
    21061 acgctgatgt tcgccgcgct gtacggtacc ggcgtccggc cgggcgacga attcttctgc
    21121 ccgtcctcgc ccgcctgggg acacggcctg tggcacggga cgctggcgcc actggcattg
    21181 ggtgtcacca ccggcacctt tgccgggcgc ttcgatgcga cgcggctgat gaaggcgctg
    21241 caggattaca ggatcacgaa catgtcggcg gccgctaccc attaccggat gatgaacaat
    21301 tcggggcggg ccggggactt cactttctcg atcaggaagt tgtcctacac gggcgagccc
    21361 atcgatccgg tgacgctcga gttcatcgac aacaccttcc atgtgccggc gtgcagcatg
    21421 tatggcacca ccgagatcgg cgtggtgctg gtcaactatc ccggcgcgag tgacttcgcg
    21481 gtcaagccgg gctcgttggg caagcccgta ccgggtctga agctccaggt gcagggcccg
    21541 gacgggaccc cgacggcacc gggcgtcgtc ggcgagctga tgttgtggcg gcgggaccgc
    21601 tgggagacca ccaaggatct cgcgaggatc gacgaggacg gttatttcta tcatgccggc
    21661 cgcgcggacg acgtgatcat atccgccggc tggacgatga gcgcggtgga gatcgagaac
    21721 accctgctca agcatccgga tgtcagggag gcggccgtca tcggcgtgcc ggatgccacg
    21781 cgcggacagg tcgtcaaatc cttcattgtc accgaccgcc cgcagagcgc cgagtttgtc
    21841 gacgaactga agaacttcac gcgggagcgg ctcagccagc acgaattccc gcgtcacatc
    21901 gaatttgtga gtgagcttcc caagacgcca gccgggaaga ttcaccgcaa ggtcctccgc
    21961 gatcgcgaag ccgccgcggc ggccgcgagc gccaactgaa accccaggag aagacgcatg
    22021 tccaccagca ccaacaagtt cccgaaaatc accgaacagg gtctcgacga tctgcgcaag
    22081 cgcatcggcg tgaagatcac cgatacgctc gagccgtgga actacgaggc aacgcgtgac
    22141 gcgatccgcc actacgccca tggcatcggc gacgacaacc cgctgtggtg cgatccggaa
    22201 tacgccgcga agaccaggta cggcaccatc gttgccctgc cgagcttcat gttcacgacc
    22261 agccgcatca tctcgggcta ttgcggtggt ctttcgggtg tgcatgccat gtgggccggc
    22321 gccgactgga catggcacaa gccggtgctg cgcaacgacg tgatcagcac cgacgcgtgg
    22381 ctcaaggatc tggtcgagca tcagaccaag ttcgccggcc gcagcttcca gcagatctat
    22441 cacgtcgact tcttcaacca gcacggtgac aaggtcgctg aaggcgacag ctgggtgttc
    22501 cgtaccgacc gcgacgaagc gcgcgagcgt ggcacgaagt acaccgaagc ccgtggccgc
    22561 gtcgagcgct acaccgagga gcagctcgcg gagttcacgc gcctctacca gaccgaggaa
    22621 atccgtggcg cgaccccgcg ttactgggaa gacgtgaagg aaggcgaagc gctgccgcgc
    22681 atgatgaagg gcccgatgac ggtcaccggc ttcatctgct atgcgcaggg ctggggcggt
    22741 ctctacatcc gtgccaacaa gctcgccaac aagatgcagc aggcgcaccc ggggctgggc
    22801 atccgcaacc gcttcaatgt gccggattgc cccgagcgcg tgcactggga cgaggagttc
    22861 gcgctcgagg tgggcgcgcc gggcgcctac gactacggtc cggagcgttg ctcgtggctg
    22921 acgcatcacg tcaccaactg gatgggcg
//
LOCUS       DAORUQ010000027        22101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1938_length_22101_cov_6.411775, whole genome shotgun sequence.
ACCESSION   DAORUQ010000027 DAORUQ010000000
VERSION     DAORUQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 22101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22101
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_22101_cov_6.411775"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /gene="ftsA"
                     /locus_tag="PLW13_04235"
     CDS             complement(<1..748)
                     /gene="ftsA"
                     /locus_tag="PLW13_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQY69610.1"
                     /translation="MGKAMSGKMIVGLDIGTSKVVAIVGEVAPDGTLKIVGLGSHASR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCQIHSVYAGIAGSHIRSLNSHGIVAIRDR
                     EVYAHDVERVIDAAQAVAIPADQKILHILPQEYVIDSQEGIKEPLGMSGVRLEAKVHV
                     VTCAVNASQNIEKCIRRCGLEVEEIILEQLASSYAVLTEDERELGVCLVDIGGGTTDI
                     AIFTEGAIRHTGVIPIAGDQVTNDIAMALRT"
     gene            complement(749..1594)
                     /locus_tag="PLW13_04240"
     CDS             complement(749..1594)
                     /locus_tag="PLW13_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQY69611.1"
                     /translation="MSALLLRMSGHAGSRRGATRRGAQPRAARAAWLVPALIVALLLA
                     AGEMGFSALREVANVPIKQVTVNGDFRFLDKAQVEEIMLPHLGTGYFMVDLAGIRDDL
                     KRLPMVHEVTVRRAWPDRLMVFITEQVPVLRLGEDAYLNPYAEVFRPAAALPGLELPL
                     VEGPAGSEKLLLDRFDAFAELLEPTGLRIARLALDGKHAWRITLDNGSELLLGRREVE
                     RKARIVSRLLAGEWAADRDRIERLDMRYSNGVAVAWKGEAPRAHRPGGTAAAGPVPAA
                     RPPNQ"
     gene            complement(1591..2553)
                     /locus_tag="PLW13_04245"
     CDS             complement(1591..2553)
                     /locus_tag="PLW13_04245"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQY69612.1"
                     /translation="MRRFDIDRAALRGPVAVLMGGTSAEREVSLESGRAVHAALTHRL
                     DGVLAIDAGADVLERLIAGGVAHVFIALHGRGGEDGTMQGALQTLGISYTGSGVLACA
                     LAMDKLRCKRFWQAEGIATPDFAVVDATANYDELAARLGPRLIVKPASEGSSIGITPV
                     DNAAELRAAVALASGYDSCVLVERRVMGPEYTVAIVDGEILPSIRLETPRTFYDYEAK
                     YQLDTTRYLCPSGLDARDERALAALAARAFESLGCSGWGRVDVMRDAASGEFAVLEVN
                     TAPGMTSHSLVPMAARAVGIEFDDLVVRIFSASLARAARGGVAG"
     gene            complement(2550..3986)
                     /gene="murC"
                     /locus_tag="PLW13_04250"
     CDS             complement(2550..3986)
                     /gene="murC"
                     /locus_tag="PLW13_04250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQY69613.1"
                     /translation="MMELKYAPVPEMRRIGHIHFVGIGGAGMCGIAEVLRNQGYDVSG
                     SDAAASPALERLRGLGVAVHLGHRAENIAGADVVVASSAVTGENVEVAAARERRIPVV
                     PRAEMLAEIMRFRHGIAVAGTHGKTTTTSIIAAIFAEAGLDPTFVIGGRVNAVQANAR
                     LGDSRYLIVEADESDASFLHLQPMVAVVTNIDADHMGTYGGDFERLKRTYVEFLHNLP
                     FYGLSVLCIDDPAVASIIADVTRHVITYGASEQADYRLCDFSQHEGRSGFTVKRPGGR
                     EPLRLQLGLPGRHYALNATAAVAVATDEGIADAAIVRGIAGFQGVGRRFENHGEQPCR
                     DGSFLLVDDYGHHPTEVAANLAAVRAGWPARRLVMVYQPHRYSRTRELYEDFVRVLSQ
                     VDVLVLLDVYGAGEDPIPGADGRSLSRSIRQRGKLDPIFVEDVHDVMDVLDTVLQPGD
                     FLLLQGAGSVGRLVAEVLARTAGGDART"
     gene            complement(3983..5068)
                     /gene="murG"
                     /locus_tag="PLW13_04255"
     CDS             complement(3983..5068)
                     /gene="murG"
                     /locus_tag="PLW13_04255"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQY69614.1"
                     /translation="MPERSPLVLITGGGTGGHVFPGLAAAAALTARGARVHWLGTHAG
                     MESRLVPAAGIPISFLRVSGVRGKSRSQQLLAPLLLLVAIVHALLLVLKLRPACMLGT
                     GGFVAGPGGIAAWLLRVPVVVHEQNAVAGTTNRLLARIAARILLGLPGPFAEHPRARL
                     AGNPVRAAIAAIPVPAERLAGRAGPLRLLVIGGSQGARVLNETLPAALALLAAGERPE
                     VWHQGGAREFEACRERYRERGVAARTTAFIDDMVAAYDWADVVVCRAGALTVSELAAA
                     GLAAILVPLPTAIDDHQSMNARWLADAGAALAVAQAAFTPEWLAAQLSRWSDARDDLL
                     LMATRARALAMPRAADTVAANCLEVAR"
     gene            complement(5061..6239)
                     /gene="ftsW"
                     /locus_tag="PLW13_04260"
     CDS             complement(5061..6239)
                     /gene="ftsW"
                     /locus_tag="PLW13_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQY69615.1"
                     /translation="MTMTQTEAVLMRKRRALDHALLALVAALAAAGFVMMGSASMDYA
                     GEQYGNPFYHIIRHGIYLLLGAATMYVTLQVPVKLWQDAGPALLVAGMAILALLLVPG
                     IGREVNGATRWIGFGPVNLQPSELMKLFFVVYLAGYMTRRQRELQNSWSGMLKPLGLL
                     GAVVLLLLLEPDFGAAVVIAGAAMAMLFLGGARLLPFIVLMLLALVGAAVMAVWSPYR
                     MQRLITFLDPWSDQFASGYQLTQALIAFGRGEVLGVGLGNSVQKLFYLPEAHTDFVFA
                     IIAEELGLLGCVAFIALFAALIARILWIGRTAARKQQLFSAHVCFGVALLVAAQVFIN
                     IGVNAGLLPTKGLTLPFLSYGGSSLLILFAMMGLILRIEVELRGAPVAATAPWRLRHA
                     "
     gene            complement(6241..7602)
                     /gene="murD"
                     /locus_tag="PLW13_04265"
     CDS             complement(6241..7602)
                     /gene="murD"
                     /locus_tag="PLW13_04265"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQY69616.1"
                     /translation="MGNTGGEARRVVVGMGQTGLSCARFLAGRGLSFAVVDSRVEPPL
                     LARFRAEFPQVQLRTGAFDAAFLATADELILSPGVDPAEPAIGAARAAGAAVLGDIEL
                     FHREARAPVVAITGTNAKSTVTTLVWLMAQEAGLQVACGGNLGTPALDLLAPQVALYV
                     LELSSFQLETVVDFRADVAAILNIAPDHLDRYPDMQAYRAAKLRIYRGARCVVFNRDD
                     AMTVPGAQDGQRRLSFGAGSAQAGNYGLLVRDGVEWVACGTEALIAARELGLRGRHNV
                     LNSLAALALAGAAGIARPAQLAVLRSYRGLPHRCQLVARTDSIDFYNDSKGTNVAAAV
                     AALRGLAGEGGGRIVLIAGGAAKESDFTLLAVELARAGRAAVLIGSSAGLLAEALAGT
                     VPLARAVDMEDAVARARALAQPGDTVLLSPACASFDMFRNYEERGDEFTRAARALAGA
                     EAL"
     gene            complement(7620..8702)
                     /gene="mraY"
                     /locus_tag="PLW13_04270"
     CDS             complement(7620..8702)
                     /gene="mraY"
                     /locus_tag="PLW13_04270"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQY69617.1"
                     /translation="MLLLLADWLSEYFTVFKVFQYLTFRGILGTLTALAITLLVGPAM
                     IRRLNVRQIGQSVRSDGPQSHLSKAGTPTMGGALILVGIVTSTLLWGDLGNRYVWVVL
                     AVTVAFGAVGWIDDYRKVVEKDSRGLPARWKYFWQSLAGLGAALYLYASAASPAETQL
                     IVPFFKQVTLALGPLFVVLAYFVIVGASNAVNLTDGLDGLAIMPSVLVAGALAVIAYL
                     AGHSEFAPYLHIPYIAGAGELVVFCGALAGAGLGFLWFNTYPAMVFMGDVGALALGAA
                     LGVIAVIVRHEIVFFIMGGVFVMETVSVMLQVASFKLTGRRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIITVVLVLFGLATLKLR"
     gene            complement(8702..10084)
                     /gene="murF"
                     /locus_tag="PLW13_04275"
     CDS             complement(8702..10084)
                     /gene="murF"
                     /locus_tag="PLW13_04275"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQY69618.1"
                     /translation="MMRAIGLAEFARFLGLPAPARDAAFDAVSTDTRTLAPGSLFVAL
                     RGENFDGHDFVAEAVRRGAGALLCSRPVDAAVPVLQVADTLVALGELGRFNRSLFTGT
                     VVALTGSAGKTTTKEMIAAILGRAGSVLATAGNLNNEIGVPLTLLALHPALRYAVIEM
                     GAAKPGDIRYLGALVRPDVAVLTNALPAHLQGFGSLQAVAATKGEIYDALGPDGVAVL
                     NVDEPFADAWLARIGARTVLRASARGAVDADVRAEDVMMHEGNASFRLATAAGAVPIA
                     LGVPGVQQVANAVCAAAAALALGLGPEAIRDGLESLTPVSGRMQRRRGARGCVLIDDS
                     YNANPDSVRAAIDSLATFGGTRMLVLGNMAELGAQSRELHREAGAHARARGIERLLAC
                     GPHADAVAEGFGPGATVLGSREEVAAACREFDRAGSVILVKGSRSAGMEAVVAALAQA
                     EPGAAGTGRH"
     gene            complement(10081..11574)
                     /locus_tag="PLW13_04280"
     CDS             complement(10081..11574)
                     /locus_tag="PLW13_04280"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQY69619.1"
                     /translation="MSNPRDPVFAERALEDLLPELAGTPSGALRVGGVCLDSRRVSAG
                     DAFIALRGARHDGAAYVDEAARRGARVVLFEQGDALAGTTLEAIGVSRLGERVSEIAG
                     RFYGTPSESMALIGVTGTNGKTTCTQLIAQAFELLGIRCGVVGTLGWGFPGRLDATTH
                     TTPDAVSLQQMLAGLRAGGATVAALEVSSHALKQGRVRGLSFATAIFTNLTHDHLDYH
                     GSFEDYGASKQILFASERLSSAVVNSDDPFGRRLLDALPRGVDALSYGVAHAGARVRA
                     IDPVFSAEGLAAQVVTPWGRGTLSAPLLGHFNLHNALAALSALCVHGVAFDDALAVFP
                     RLSPVAGRMERVAAADGPLVIVDYAHTPDALEQTLRALRQHCPGRLWCVFGCGGDRDR
                     AKRPLMGRIAWEHADELLITSDNPRSEDPAAIIAEIATGLPEHGAAHEPDRAAAIRSA
                     IAAAGPGDCVLIAGKGHEDYQEVSGVRTPFSDAGIARAALAAWSAAS"
     gene            complement(11571..13190)
                     /locus_tag="PLW13_04285"
     CDS             complement(11571..13190)
                     /locus_tag="PLW13_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQY69620.1"
                     /translation="MLDTERGYRFLQSQGDARSLRVEPIPAHRGIISDRNGEPLAIST
                     PVQTLWANPKQLVEKIEDWRPLASALGVAPDELERRIGSVATREFVYLRRRMTPADAA
                     RVMALGIPGVYSRREYQRFYPAGQTAAHVVGFTNVDDRGQEGIELAYDEWLTGVPGSK
                     QVIKDLYGHVIRDVQELSPARPGRALDLSLDLRIQYLAHRELKKAIESASAESGCVVV
                     LDARSGEILAMANQPTYNPNNRGGDIGSALRNRAITDLVEPGSTMKPLTMIAALESGK
                     YHPGMTMETSPGYIRVGRKTFRDHHNYGLLTLGGVITKSSQVGTVKIAMSLEPDVIRD
                     VFVRAGLGQSVGTGFPGESVGVLPDYRRWSDVARANLAFGYGLSVTPLQLARTYSILA
                     AGGVEHPLTLLRGRGTPEAKPVYKPEIIAQLVDMMKTVTQPGGTALKAAIKGYTVAGK
                     TGTVHRIGPGGYEANRYVSLFAGFAPATDPRIVAVVIVSDPRGGVYYGGAIAAPVFST
                     VVAGALRLMDVPPDNLPEILVAERDAGRTPT"
     gene            complement(13295..13600)
                     /gene="ftsL"
                     /locus_tag="PLW13_04290"
     CDS             complement(13295..13600)
                     /gene="ftsL"
                     /locus_tag="PLW13_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQY69621.1"
                     /translation="MAAEEPARDAAAPDRRPLLAALLLMLAVLSSFGVIYAAHESREL
                     FRQLEQARRDENEIQIEWRQLLLERGTLSSHARVEAMAGSALQMVPVEGELRVLVVE"
     gene            complement(13590..14525)
                     /gene="rsmH"
                     /locus_tag="PLW13_04295"
     CDS             complement(13590..14525)
                     /gene="rsmH"
                     /locus_tag="PLW13_04295"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQY69622.1"
                     /translation="MHTPVMLQEAVDFLVTDSAGSYVDGTFGRGGHTRAILGRLAPAA
                     RLLAIDHDPEAIAAGRSQFSDDPRLVIEHASFDELQTLLERHDMAGRVHGVLLDLGVS
                     SPQLDDPRRGFSFLQDGPLDMRMNPGRGISAAQWLARARAEDIAQVLFEYGEERFARR
                     MAAAIVRERSVEPIETTARLAQIVAAANPAWEKAKHPATRAFQAIRIYINGELDALAS
                     VLRQALDALLPGGRLVVISFHSLEDRIVKRFMRDHSRAAQQPGLPAYLQPKLKRLGKA
                     LRPSEEEVERNPRARSAVLRVAEKLTVAGIAVDGR"
     gene            complement(14551..15006)
                     /gene="mraZ"
                     /locus_tag="PLW13_04300"
     CDS             complement(14551..15006)
                     /gene="mraZ"
                     /locus_tag="PLW13_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQY69623.1"
                     /translation="MFRGVHHLSIDPKGRMAMPARHRDRLQAISAGQLVATIDTQSKC
                     LLVYPLPTWEDLEREIQALPTLNPVVRRFQRLVIGYASDLECDGSGRVLVPPALREYA
                     GLDKKAVLVGQGNKMELWDEDTWLAERDKWLQMASVEGAVPDEMLRLSL"
     gene            complement(15313..15720)
                     /gene="rnpB"
                     /locus_tag="PLW13_04305"
     ncRNA           complement(15313..15720)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLW13_04305"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(15736..16593)
                     /gene="rsmI"
                     /locus_tag="PLW13_04310"
     CDS             complement(15736..16593)
                     /gene="rsmI"
                     /locus_tag="PLW13_04310"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQY69624.1"
                     /translation="METPAVLFVVATPIGNLGDLSHRAAQVLAAADLIAAEDTRRCRQ
                     LLNHLGISRPVVAYHDHSDARRLEHVLQLLRDGSSVALVSDAGTPLISDPGYRLVHDA
                     LAAGISVVAVPGPCAAVAALSVAGLPSDRFVFEGFLPARQAARQARLEGLGMETRTLI
                     FYESPRRILDTLRDLAAVFGGERRCALARELTKVHETLLSGTVNEVLERVAADPEQQL
                     GEIVLVLAGAEAVVADETAISTGALLEALLKRLPLKEAVACAVEISGRPRNEVYRAAL
                     ALRPGGDAG"
     gene            16669..18639
                     /locus_tag="PLW13_04315"
     CDS             16669..18639
                     /locus_tag="PLW13_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQY69625.1"
                     /translation="MNRAFVRPLLVAALLVAAGCSDQPEKPSSEHIPLPEKPATQPAE
                     PADEVERLLALAARSDGAAAREARLDAAAELVRRGDLARAATLLDELEAASLDSAQWI
                     RHSLLRARLELEGGDAAAALARLDNPALQAEGAAVGRALQVELALTRADTLAALQQHL
                     ESARERVRVQPLLSDETARHDNTHAIFAALAGADMAALERETGVAASDDWRGWLELTA
                     LVRDLRRGPDEQLGALASWEQRYSLIAPLQPAVNELLPAIRERVRQPARVALLLPLSG
                     RAAPGATAVLNGYLAEHMRQLAAGEQPVPVAVVDTAAAPGGFAAAYASAVADGADMVI
                     GPLLKEDLAAFGPTLAPSVTTIALNFADSANSDGDMLYEFGLDGADEVAQLATAARAR
                     GWAHAIVVADDSDTSRRQAQEFERLWNGGDTRVLGTLYLADINEFRRRFEEALLFEQS
                     RQRSAALSRLLGLALVSEPRRRQDLDLIVMFSGPVGARSIRPLLSFLYAGDLPVWSTS
                     QANGARPDRRNDRDLEYVRFLDLPWFSGAERPLRETLRRSVPAGGLERFAALGADACR
                     LQSRIGLLDWNGAAGLGGATGELVLDGTRRLHRKSQWYVFRDGLAAPEIQRTALLATP
                     LNRQSTEGATPWTDTTSAAPPPPPDAPQKTAP"
     gene            18573..18962
                     /locus_tag="PLW13_04320"
     CDS             18573..18962
                     /locus_tag="PLW13_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQY69626.1"
                     /translation="MDRHHQRGTSAATGRAAEDRALKELGAAGLRLVARNFRCRLGEI
                     DLVMREGDTLVFVEVRQRQSARFGDASESVTVAKQRKLLAAAALFVAWHPALRDLPMR
                     FDLFALDGNAHGSTTVPGAKWLRGIIS"
     gene            18981..19580
                     /locus_tag="PLW13_04325"
     CDS             18981..19580
                     /locus_tag="PLW13_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQY69627.1"
                     /translation="MSLDMLNEPLQALFASHVDTVYRALDVMYPEIHRAVELLCDSLL
                     ADRRVLVCGSGTGTALGQVFCTNLLNRAQIDRPGLPVIAIGSDPAATSAIAESYGISE
                     VQSRQIRALGQAGDALMVIAGSPRGAGLSPAVRAAHAREMRVIALTGGEGGEPAAALG
                     PADIELRVPSDDPYRVSESQLLLLNALSALIELRLFGEA"
     gene            complement(19585..19959)
                     /locus_tag="PLW13_04330"
     CDS             complement(19585..19959)
                     /locus_tag="PLW13_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HQY69628.1"
                     /translation="MKSSRPYLVRALYEWILDNACTPYLLVDATVAGVEVPQQYVKNG
                     QIVLNVAPGAVAGIDMSNDDIRFRGRFGGVAIDVFVPIGAVLGIYARENGQGMVFEPE
                     QAPPPSSDDEPRSGKPSLRVVK"
     gene            complement(19999..20658)
                     /locus_tag="PLW13_04335"
     CDS             complement(19999..20658)
                     /locus_tag="PLW13_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQY69629.1"
                     /translation="MRPGRDIRAVLARRTAMTLFSDARCHYSHRVRIVLAEKGVAAEL
                     IDVDGRDYPPALAEINPYSSLPTLVDRELVLYQPGIMMEYLDERFPHPPLLPVYPVAR
                     GECRQLMYRLERDWSSRVDVMESPASPPQAVAKARREVGDELMAVAPVFAEMPFFMSE
                     EFSLADCCLCAILWRLPALGIVLPNIRQTRPLHDYMTRMFARDGFRASLSETEKEIRR
                     R"
     gene            complement(20707..21474)
                     /locus_tag="PLW13_04340"
     CDS             complement(20707..21474)
                     /locus_tag="PLW13_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HQY69630.1"
                     /translation="MAFLPLKAVGAEAAFDCGTIPCDEFSPALNDKASLQRGAKYYMN
                     YCMGCHSLKYARFERTATDLGIPLPLAEQNLIFDDAKIGELMENAMPVPLAKKWFGAP
                     PPDLTLISRARSPEYIYTYLRNFYRDDSPARPWGVNNRVFPDVAMPHVLIELQGLPEC
                     APGPVLAHNGGIKRDPLTGEDLLEAPCGRLKVAQAGSMTVEEFDTAMADLASFLAYVA
                     EPMALERKRMGIYVLLFLAFFGVFAYLLNREYWKDVH"
     gene            complement(21532..22101)
                     /locus_tag="PLW13_04345"
                     /pseudo
     CDS             complement(21532..22101)
                     /locus_tag="PLW13_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT         3539 a         7878 c         7432 g         3252 t
ORIGIN      
        1 gcgtgcgcag cgccatcgcg atgtcgttgg tgacctggtc gccggcgatc gggatgacgc
       61 cggtgtggcg gatcgctccc tccgtgaaga tcgcgatgtc cgtggttccg ccgccgatgt
      121 cgaccaggca gacaccgagc tcgcgctcgt cctcggtcag caccgcgtag ctcgaggcca
      181 gctgctcgag aatgatttcc tcgacctcga gcccgcagcg gcggatgcac ttctcgatgt
      241 tctgcgaggc gttgaccgcg caggtcacca cgtgcacctt ggcctcgagc cgcacgcccg
      301 acatgcccag cggctccttg atgccttcct ggctgtcgat cacgtactcc tgcggcagga
      361 tgtgcaggat cttctggtcg gcgggaatcg ctacggcctg cgccgcgtcg atcacgcgct
      421 ccacgtcgtg cgcgtagacc tcgcggtcgc ggatcgcgac gatgccatgc gagttgaggc
      481 tgcggatatg gctgcccgcg atcccggcat agaccgaatg gatctggcag ccggccatca
      541 gctcggcctc ctcgacggcg cgctgtatcg actgcacggt ggactcgatg ttgaccacca
      601 cgcccttttt gaggccgcgc gatgcgtgcg atccgagacc gacgatcttc agcgtgccgt
      661 ccggtgccac ctcgccgacg atcgccacca ccttcgaggt gccgatatcg aggccaacga
      721 tcatctttcc gctcattgct ttccccattc attgattcgg cgggcgcgcc gcgggaaccg
      781 ggcccgccgc tgctgtgccc ccgggccggt gcgcccgcgg cgcttcgccc ttccacgcca
      841 ccgccacgcc attgctgtaa cgcatgtcca gtcgctcgat gcggtcacgg tcggcggccc
      901 attcgccggc gaggagccgc gagacgatgc gcgccttgcg ctccacctcg cgccgcccga
      961 gcagcagctc gctgccgttg tcgagcgtga tgcgccacgc gtgcttgccg tcgagcgcga
     1021 gccgcgcgat gcgcagcccc gtgggctcga gcagctcggc gaacgcatcg aagcgatcga
     1081 gcagcagttt ctcgctgccc gcgggtccct cgaccaaggg cagctccagt ccgggcagcg
     1141 cggcggcggg ccggaagacc tcggcgtacg ggttgaggta ggcgtcctcg cccaggcgca
     1201 gcaccggcac ctgctcggtg atgaacacca tcagccggtc gggccaggcg cggcgcacgg
     1261 tgacctcgtg caccatcggc agccgcttca ggtcgtcgcg gatccccgcg agatcgacca
     1321 tgaagtagcc cgtgccgagg tgcggcagca tgatctcctc gacttgcgcc ttgtcgagga
     1381 agcggaaatc cccgttcacc gtcacctgct tgatcggcac gttggcgacc tcgcgcagcg
     1441 cgctgaagcc catctccccg gcagccagca gcagcgccac gatcagcgcc ggcaccagcc
     1501 atgccgcgcg cgcggcgcgc ggctgcgcgc cacggcgggt cgcgccgcga cgcgagcccg
     1561 cgtggcccga catgcgcagc agcagcgcgc tcacccggcg actccgccac gggcggcgcg
     1621 cgcgaggctg gcgctgaaga tgcgtaccac caggtcgtcg aattcgatgc ccacggcgcg
     1681 tgccgccatc ggcaccaggc tgtgactggt catgcccggc gcggtgttca cctcgagcac
     1741 ggcgaactcg ccgctggcgg catcgcgcat cacgtccacc cggccccagc cgctgcagcc
     1801 caggctttcg aaagcgcgcg cggcgagcgc ggcgagcgcg cgttcgtcgc gggcatcgag
     1861 gcccgacgga cacaggtagc gcgtggtgtc gagctggtac ttggcctcgt agtcgtagaa
     1921 cgtgcgcggc gtttcgagcc gtatcgacgg caggatctct ccatcgacga tcgcgaccgt
     1981 gtattccggc cccatgacgc ggcgctcgac cagcacgcag ctgtcgtagc cgctcgccag
     2041 cgcaaccgcc gcgcgcagct cggcggcgtt gtcgaccggc gtgatgccga tgctcgaacc
     2101 ctcgctggcc ggcttcacga tgaggcgcgg cccgaggcgc gcggcgagct cgtcgtagtt
     2161 cgccgtggca tccaccaccg cgaagtccgg cgtcgcgatt ccctcggcct gccagaagcg
     2221 cttgcaccgc agcttgtcca tcgcgagcgc gcaagccagc acgccgctgc cggtgtagct
     2281 gatgccgagc gtctgcaggg cgccctgcat cgtgccgtcc tcgccgccgc gcccgtgcag
     2341 cgcaatgaac acgtgcgcca ccccgccggc gatcaggcgc tcgaggacgt cggcgcccgc
     2401 atctatagcg agcaccccgt cgagccggtg cgtcagcgcc gcatgcaccg cgcggcccga
     2461 ctcgagcgag acctcgcgct cggccgaggt gccgcccatc agcaccgcca ccggaccgcg
     2521 caatgccgcc ctgtcgatat cgaaacgtct catgtgcgcg catccccgcc ggccgtgcgg
     2581 gccagcacct cggcgaccag cctgcccacg ctgcccgcgc cctgcagcag caggaagtcg
     2641 ccgggttgca gcaccgtgtc gaggacgtcc atcacgtcgt gcacgtcctc gacgaaaatc
     2701 ggatccagct tgccgcgctg gcggatgctg cggctcaggc tgcgtccgtc ggcgccggga
     2761 atcggatcct cgccggcgcc gtacacgtcc agcagcacca gcacatcgac ctgcgacagc
     2821 acccgcacga agtcctcgta gagctcgcgc gtgcgcgagt agcggtgcgg ctggtacacc
     2881 atcacgaggc ggcgagcggg ccagcccgcg cgcaccgcgg cgaggttcgc cgccacctcg
     2941 gtggggtgat gcccgtagtc gtcgaccagc aggaagctgc cgtcgcggca gggctgctcg
     3001 ccgtgattct cgaaacgccg ccccacgccc tggaatccgg cgatgccgcg cacgatcgcc
     3061 gcgtccgcga tgccctcgtc ggtggccacc gccaccgcag ccgtggcgtt cagcgcgtag
     3121 tggcgcccgg gcaggcccag ctgcagccgc agcggttcgc gcccgccggg acgcttcacc
     3181 gtgaacccgg acctgccctc gtgctggctg aaatcgcaca ggcggtaatc ggcctgctcc
     3241 gacgcgccgt aggtgatcac gtggcgcgtg acgtcggcga tgatcgacgc gaccgcgggg
     3301 tcgtcgatgc acagcacgct caggccgtag aacggcaggt tgtgcaggaa ctcgacgtag
     3361 gtccgcttca gccgctcgaa gtcgccgcca taggtgccca tgtggtcggc atcgatattt
     3421 gtgaccacgg ccaccatcgg ctgcaggtgc aggaacgagg cgtcgctctc gtcggcctcg
     3481 acgatgagat aacggctgtc gcccagccgc gcgttggcct gtaccgcgtt cacgcgcccg
     3541 ccgatcacga aggtgggatc cagcccggcc tcggcgaaga tcgcggcgat gatgctggtg
     3601 gtggtggtct tgccgtgcgt gcccgccacc gcgatcccgt ggcgaaaacg catgatctcg
     3661 gcgagcatct cggcgcgcgg caccaccggg atgcggcgtt cgcgcgcggc cgcgacctcg
     3721 acgttctcgc cagtaacggc gctggaggcg accaccacat cggctcccgc gatattctcg
     3781 gcacggtgcc ccaggtgaac cgcgacgccc agcccgcgca ggcgttccag cgccggcgag
     3841 gccgcggcgt cggagcccga gacgtcgtag ccctggttgc gcagcacctc cgctattccg
     3901 cacatgcccg caccgccgat accgacgaaa tggatgtgcc cgatgcgtcg catctccggc
     3961 accggcgcgt atttgagttc catcatcgcg ccacctccag gcaattggcc gcgaccgtgt
     4021 cagcggcccg gggcatcgcc agcgcgcgcg cgcgcgtggc catcagcaac aagtcgtcgc
     4081 gggcgtcgga ccagcgcgag agctgcgcgg cgagccactc tggggtgaac gcggcctgcg
     4141 cgacggcaag cgccgcgccg gcatcggcga gccagcgcgc gttcatgctc tggtggtcgt
     4201 cgatggcggt gggcagcggc accaggatcg cggcgagccc cgcggcagcg agctcgctca
     4261 ccgtgagcgc gccggcgcgg cacaccacca cgtccgccca atcgtaggcc gcgaccatgt
     4321 cgtcgatgaa cgcagtggtg cgcgccgcga cgccgcgctc gcggtaacgc tcgcggcagg
     4381 cctcgaactc gcgcgcgccg ccctggtgcc agacctcggg acgctcgccc gccgccagca
     4441 gcgccagcgc cgccggcagg gtttcgttca gcacgcgcgc gccctggctg ccgccgatca
     4501 cgagcaggcg caggggcccg gcgcgccctg ccaggcgctc ggcgggcacc gggatcgcgg
     4561 cgatcgccgc acgcacggga ttgccggcga ggcgggcacg cggatgctcc gcaaacggac
     4621 ccggcagacc gagcaggata cgcgcggcga tgcgcgcgag caggcgattc gtggtcccgg
     4681 cgacggcgtt ctgctcgtgc accaccaccg gcacgcgcag cagccaggcc gcgatgccgc
     4741 cgggtccggc cacgaagccg ccggtgccga gcatgcaggc cggccgcagt ttcagaacca
     4801 ggagcagcgc gtgcacgatc gccacgagca gcagcaacgg tgccagcagc tgctgactgc
     4861 ggctcttgcc gcgcacgccg ctcacgcgca ggaaggaaat gggtatgccg gccgccggga
     4921 ccaggcgcga ctccatgccg gcatgcgtcc cgagccagtg cacgcgtgcg ccgcgcgccg
     4981 tcagggccgc cgcagccgcg agcccgggga acacgtgtcc gccggtgccg ccaccggtga
     5041 tgaggaccag gggggagcgc tcaggcatgg cgcagcctcc acggtgccgt cgccgccacc
     5101 ggcgcgccac gcaactcgac ctcgatgcgc aggatcaatc ccatcatggc gaacaggatc
     5161 agcaagctgc tgccgccgta gctgagaaac ggcagcgtga gccccttggt cggcagcagc
     5221 cccgcgttca cgccgatgtt gatgaacacc tgcgcggcga ccagcagcgc caccccgaag
     5281 cacacgtgcg cggaaaagag ctgctgcttg cgcgcggccg tgcgcccgat ccagaggatg
     5341 cgcgcgatca gcgccgcgaa cagcgcgatg aacgccacgc aacccagcag cccgagctcc
     5401 tcggcgatta tcgcgaacac gaagtccgtg tgcgcttccg gcaaatagaa cagcttctgc
     5461 acgctgttgc cgagcccgac gcccagcacc tcgccgcggc cgaaggcgat cagcgcctgc
     5521 gtcagctggt agccgctggc gaactggtcg gaccacggat cgaggaaggt gatcagtcgc
     5581 tgcatgcggt acggcgacca caccgccatg acagcggcgc cgacgagggc gaggagcatc
     5641 agcacgatga acggcagcag ccgcgcaccg ccaaggaaca gcatggccat cgcggcgccg
     5701 gcgatcacca ccgcggcgcc gaagtcgggc tccagcagca gcagcaggac cacggcgccg
     5761 agcagcccga gcggcttcag catgcccgac cagctgttct gcagctcgcg ctgacgccgc
     5821 gtcatatagc cggccaggta cacgacgaag aacagcttca tcagctccga gggctgcagg
     5881 ttcaccggac cgaaaccgat ccagcgtgtc gcgccgttga cttcgcgtcc gatccccggc
     5941 accagcagca gcgccagtat cgccatgccg gccaccagca gcgcaggccc cgcgtcctgc
     6001 cacagcttca ccggaacctg cagcgtcacg tacatcgtcg cggcgccgag caacaggtaa
     6061 atgccgtggc ggatgatgtg gtagaagggg ttgccgtact gctcgcccgc gtagtccatc
     6121 gacgcggatc ccatcatcac gaaacccgcc gcggcgagcg ccgcgaccag tgccagcagc
     6181 gcgtggtcga gcgctcggcg cttgcgcatc agtaccgctt cggtctgcgt catcgtcatc
     6241 tcagagcgcc tccgcgccgg caagtgcgcg cgccgcgcgg gtgaactcgt caccgcgctc
     6301 ctcgtaattg cggaacatgt cgaagctggc gcaggcgggc gagagcagca cggtgtcgcc
     6361 cggctgcgcg agcgcacgcg ccctggcgac cgcgtcttcc atgtccaccg cacgcgcgag
     6421 cggcaccgtg cccgcgagcg cctcggccag cagcccggcg gaactgccga tcagcacggc
     6481 ggcgcgaccc gcgcgcgcga gctcgacggc cagcagcgtg aagtcgctct ccttggccgc
     6541 gccgccggcg atcagcacga tgcgcccgcc gccctcgcct gcgagcccgc gcagtgcagc
     6601 cacggcggcg gcgacgttgg tgcccttgga atcgttgtag aaatcgatgc tgtcggtgcg
     6661 tgccacgagc tggcaacggt gcggcaggcc gcggtaactg cgcagcaccg cgagctgcgc
     6721 gggacgcgca atgcccgcgg ccccggccag cgccagcgcc gccaggctgt tgagcacgtt
     6781 gtgccgcccg cgcagaccga gctcgcgcgc ggcgatcaac gcctcggtgc cgcaagccac
     6841 ccattcgaca ccgtcgcgca cgaggagccc gtagttgccc gcctgtgcgc tgcccgcgcc
     6901 gaaactcagg cgccgctggc cgtcctgcgc gccgggtacc gtcattgcgt cgtcgcggtt
     6961 gaacaccacg cagcgcgcgc cgcggtagat gcgcagcttc gccgcgcggt aggcctgcat
     7021 gtcgggatag cggtcgaggt ggtcgggcgc gatgttgagg atggcggcca catcggcccg
     7081 aaaatcgacg acggtctcga gctggaagct cgacagctcg agcacgtaca gcgccacctg
     7141 cggcgcgagc agatcgagtg ccggcgtgcc gaggttgccg ccgcaggcca cctgcaaccc
     7201 cgcctcctgc gccatcagcc agaccagcgt ggtcacggtg ctcttggcgt tggtgccggt
     7261 gatcgcgacc acgggcgcgc gcgcttcgcg gtggaacagc tcgatgtcgc cgagcactgc
     7321 cgcgcccgcg gcgcgtgcgg cgcctatcgc gggctcggcg ggatcgacgc cgggactcag
     7381 gatcagttcg tcggccgtcg cgaggaacgc cgcgtcgaac gcgccggtgc gcaattgcac
     7441 ctgcgggaac tcggcgcgaa agcgcgccag caacggcggc tccacgcggc tgtcgaccac
     7501 cgcgaaggac agcccgcgcc cggcgaggaa gcgcgcacag gacagcccgg tctggcccat
     7561 gccgaccacg acccgccttg cctccccgcc cgtgttgccc attgcccgtg ccgctccgct
     7621 cagcgcaact tcaacgtggc caatccgaac aggaccagca cgaccgtgat gatccagaag
     7681 cgcacgatca cgcgcggctc cggccatccc ttgagctcga aatggtggtg gatcggcgcc
     7741 atgcggaaga tgcgcctgcc cgtcagcttg aaggaggcga cctgcagcat caccgagacc
     7801 gtctccatca cgaacactcc gcccatgatg aagaacacga tctcgtggcg cacgatcacc
     7861 gcgatgacac ccagcgccgc gccgagcgcc agcgcgccca cgtcgcccat gaaaaccatc
     7921 gcgggatagg tgttgaacca caggaagccg agcccggctc cggccagcgc gccgcagaaa
     7981 accaccagct cgccggcgcc ggcgatgtag ggaatgtgca ggtagggcgc gaactcgctg
     8041 tgccccgcga ggtaggcgat taccgccagc gcgccggcca ccagcacgct cggcatgatc
     8101 gcgaggccgt cgagcccgtc ggtgaggttc accgcattgc tcgcgccgac gatcacgaaa
     8161 taggcgagca cgacgaacag cgggccgagc gcgagcgtca cctgcttgaa gaagggcacg
     8221 atcagctgtg tttcggcagg cgacgcggcg ctggcataga ggtagagcgc ggcgccgagg
     8281 cccgcgagcg actgccagaa gtatttccag cgcgccggga gtccgcgcga atccttctcc
     8341 acgaccttgc ggtaatcgtc gatccagccc accgcgccga atgccacggt gaccgcgagc
     8401 acgacccaga catagcggtt gccgagatcg ccccacagca gcgtgctggt gacgatgccg
     8461 acgaggatca gtgcgccgcc catcgtcggc gtgcccgcct tgctcaggtg cgactgcggc
     8521 ccgtcgctgc gcacggattg cccgatctgg cggacgttga ggcgccggat catcgcgggg
     8581 cctacgagca gcgtgatcgc gagcgcggtg agcgtcccga gaatgccgcg gaaagtgagg
     8641 tactggaata ccttgaacac cgtgaagtat tcgctcagcc agtccgcgag caacagcagc
     8701 atcagtgcct ccccgtcccg gcggcgccgg gttccgcttg cgccagcgcc gccaccacgg
     8761 cttccatgcc ggcagagcgc gaacccttga ccaggatcac gctccccgca cgatcgaatt
     8821 cacggcaggc cgcggccacc tcctcgcgcg agccaagcac cgtcgcgccc ggaccgaagc
     8881 cctcggccac cgcatcggca tgcggcccgc aggccagcag gcgctcgatg ccgcgcgcgc
     8941 gcgcatgcgc gcccgcctcg cggtgcaact cgcggctctg cgcgccgagc tcggccatgt
     9001 tcccgagcac cagcatgcgc gtgccgccga aggtagcgag cgaatcaatc gccgcgcgca
     9061 ccgagtcggg attggcgttg tagctgtcgt cgatcagcac gcaaccgcgc gcgccgcggc
     9121 gtcgctgcat gcgcccggac accggagtga gcgactcgag cccgtcgcgg atcgcctccg
     9181 gcccgagacc gagcgccagc gccgcggcgg ccgcgcagac cgcgttggcg acctgctgca
     9241 caccggggac accgagagcg atgggcaccg cacctgccgc ggtcgccagg cggaaactcg
     9301 cgttgccctc gtgcatcatc acgtcctcgg cgcgcacatc cgcatccacc gcgcccctcg
     9361 cgctggcgcg cagcacggtg cgcgcaccga tgcgcgcgag ccaggcgtcg gcaaagggct
     9421 cgtcgacatt cagcaccgcg acgccatcgg ggccgagcgc gtcgtagatc tcgcccttgg
     9481 tcgccgccac cgcttgcagg ctcccgaaac cctgcaggtg cgccggcagc gcattggtca
     9541 gcacggcgac gtcgggccgc accagcgcgc ccaggtagcg gatgtcgccc ggcttcgctg
     9601 cgcccatctc gatcaccgcg tagcgcagcg ccggatgcag tgcgagcagc gtgagcggca
     9661 cgccgatctc gttgtttagg ttgccggccg tggccagcac ggagcccgcg cgcccgagga
     9721 tcgccgctat catttccttg gtcgtggtct tgccggcgct gccggtgagt gcgaccacgg
     9781 tgccggtgaa cagcgaacgg ttgaaacggc cgagttcccc gagcgcgacg agggtgtcgg
     9841 cgacctgcag caccggcacc gcggcgtcga ccgggcggct gcacagcagc gcgcccgcac
     9901 cgcggcgcac cgcctcggcg acgaaatcgt gaccgtcgaa gttctccccg cgcagcgcca
     9961 cgaacaggct gcccggcgcg agcgtgcgcg tgtcggtgct cacggcgtcg aacgcggcgt
    10021 cgcgcgccgg cgcgggcagc ccgagaaaac gcgcgaactc ggcgaggccg atggcgcgca
    10081 tcatgatgcg gcgctccacg cggcaagcgc ggcgcgcgct atgccggcat cgctgaacgg
    10141 cgtgcgcaca ccgctcactt cctggtagtc ctcgtgcccc ttgccggcga tcagcacgca
    10201 gtcgccggga cccgccgcgg cgatggcgct gcggattgcc gctgcgcgat cgggctcgtg
    10261 cgcggcgccg tgctccggca ggcccgtggc gatttcggcg atgatcgccg ccggatcctc
    10321 gctgcgcggg ttgtcgctgg tgatcaacaa ttcgtccgcg tgctcccacg cgatgcgtcc
    10381 catcagcggc cgcttggcgc ggtcgcgatc gccgccgcag ccgaacacgc accacagccg
    10441 tccgggacag tgctggcgca acgcgcgcag ggtctgctcg agcgcatccg gggtgtgggc
    10501 ataatcgacg atgaccagcg ggccatcggc ggccgcgacg cgctccatgc gcccggccac
    10561 cggcgagagc cgggggaaga ccgccagtgc gtcgtcgaag gccacaccgt gcacgcagag
    10621 cgcgctgagc gcagccaggg cgttgtgcag gttgaagtgc ccgagcaacg gggcgctgag
    10681 cgtgccccgc ccccacggag tcacgacctg cgccgccaag ccttcggcgc tgaacaccgg
    10741 atcgatcgcg cgcacccgcg cgccggcgtg cgcaacaccg tagctgagcg catcgacgcc
    10801 gcgcggcagc gcgtccagca gtcggcgacc gaacgggtcg tcgctgttga ccaccgcgct
    10861 cgacagacgc tcgctggcaa acaggatctg cttgctggcg ccgtagtcct cgaagctgcc
    10921 gtggtagtcc agatgatcgt gggtgaggtt ggtgaagatc gccgtcgcga aactcagccc
    10981 gcgcacgcgg ccctgcttca atgcgtgcga ggacacttcc agtgcggcga ccgtggcacc
    11041 cccggcgcgc agcccggcaa gcatctgctg caggctgacg gcgtcggggg tggtgtgcgt
    11101 ggtcgcatcg aggcgtcccg gaaagcccca gcccagcgtg ccgaccacgc cacagcggat
    11161 gccgagcagt tcgaacgcct gggcgatcag ctgcgtgcag gtggtcttgc cgttggtacc
    11221 ggtgacgccg atcagcgcca tcgactccga cggcgtgccg tagaaacgtc ccgcgatctc
    11281 gctgacgcgc tcaccgagac ggctgacgcc gatcgcctcg agcgttgtac cggctaacgc
    11341 gtcgccctgc tcgaacagca cgacgcgggc gccgcggcgc gccgcctcgt ccacgtaggc
    11401 ggctccgtca tgacgcgccc cgcgcaaggc gatgaacgca tcgcccgcgc tcacgcggcg
    11461 gctgtcgagg cagacaccgc cgactcgcag agcgccgctc ggcgtgcccg cgagctcggg
    11521 gagcaaatcc tcgagcgcac gctcggcaaa gaccggatcc cgtggattgc tcatgtcggc
    11581 gtgcgccccg cgtcgcgctc ggccaccagg atctcgggca ggttgtcggg cggcacgtcc
    11641 atcagccgca gggcgccggc cacgacggtc gagaacaccg gagccgcgat ggccccgccg
    11701 tagtaaacac cgccgcgcgg atcgctgacg atcaccaccg cgacgatgcg cgggtcggtg
    11761 gccggcgcga agccggcgaa cagcgacacg tagcggttgg cctcgtagcc gccggggccg
    11821 atgcgatgca ccgtgcccgt cttgccagcc accgtatatc ccttgatcgc ggccttgagc
    11881 gcggtgccgc ccggttgcgt cacggtcttc atcatgtcca cgagctgcgc gatgatctcc
    11941 ggcttgtaga ctggcttggc ctccggtgtc ccgcgaccac gcagcagcgt cagcgggtgt
    12001 tctaccccgc cggccgcgag tatcgagtag gtccgcgcga gctgcagcgg cgtcaccgag
    12061 aggccgtagc cgaaggccag gttcgcgcgc gcaacatcgc tccacctgcg gtagtcggga
    12121 agcacgccga cgctctcgcc cgggaatccc gtgcccaccg actgtcccag tccggcgcgc
    12181 acgaacacgt cacggatcac atcgggctcc agcgacatcg ctatcttgac ggtgccgacc
    12241 tggctcgact tggtgatcac gccgcccagc gtcagcaggc cgtagttgtg gtggtcgcgg
    12301 aaggtcttgc gcccgacccg gatgtagccc ggcgaggtct ccatggtcat gcccgggtga
    12361 tacttgccgc tctcgagcgc cgcgatcatc gtcagcggct tcatcgtgga gcccggctcg
    12421 acgaggtcgg tgatggcgcg gttgcgcagc gccgagccga tgtcgccgcc gcggttgttg
    12481 gggttgtagg tgggctggtt agccatcgcg aggatttcgc cgctgcgcgc atcgagcacc
    12541 acgacacagc cggattccgc gctcgcgctt tcgatggcct ttttcagttc gcgatgggcc
    12601 aggtactgga tgcgcaggtc caggctgagg tccagcgcac gcccgggacg cgccgggctc
    12661 agctcctgca cgtcgcggat cacgtggccg tagagatcct tgatcacctg cttcgaaccg
    12721 ggcacgccgg taagccactc gtcgtaggcg agttcgatgc cttcctggcc gcggtcatcg
    12781 acgttggtga aacccacgac gtgcgcggcg gtctggcctg ccggatagaa gcgctggtat
    12841 tcgcgccgcg agtagacccc gggaatgccc agcgccatca cgcgcgccgc gtcggcgggc
    12901 gtcatgcgcc ggcgcaggta gacgaattca cgggtggcga cgctgccgat acgccgctcg
    12961 agctcatcgg gcgcgacccc gagtgcgctg gccagcggcc gccagtcctc gatcttttcc
    13021 acgagctgct tgggattggc ccagagcgtc tgcaccggcg tgctgatcgc gagcggctcg
    13081 ccgttgcgat cggaaatgat cccgcgatgc gccgggatgg gctccacgcg cagtgaacgc
    13141 gcgtcaccct ggctctgcag gaagcggtag ccgcgctcgg tgtcgagcac ctaaagcgat
    13201 accaggcgcc ataccagcag cagcagcagg cagccgagtc ccgcggcgac catcctgatg
    13261 cgccattcgg cgatggcagc gggaggacgg agcttcattc gaccaccagc acgcgcaatt
    13321 ccccctcgac cgggaccatc tgcagcgcgc tgccggccat ggcctcgacc cgcgcatgcg
    13381 aggacagcgt gccgcgctcg agcaggagct ggcgccattc gatctggatc tcgttctcgt
    13441 cacggcgcgc ctgctcgagc tgtcggaaca gttcgcgcga ctcgtgggcg gcgtagatca
    13501 caccgaagga cgacagcacc gccagcatca gcagcagcgc ggcgagcagc ggccggcgat
    13561 cgggcgccgc ggcgtccctg gccggctctt cagcggccat cgacagcgat tccggcaacg
    13621 gtgagctttt ccgccacgcg cagcacggcg ctgcgcgcgc gcggattgcg ctccacctct
    13681 tcctcgctcg ggcgcagcgc cttgccgagc cgcttgagct tcggctgcag gtacgccggc
    13741 aggcccggct gctgcgccgc acgcgagtga tctcgcatga agcgcttcac gatgcggtcc
    13801 tcgagcgagt ggaagctgat caccacgagc cggccgccag gcagcagcgc gtccagcgcc
    13861 tggcgcagca cgctggccag cgcgtcgagt tcgccgttga tgtagatgcg gatcgcctgg
    13921 aacgcgcgcg tcgccgggtg cttggccttt tcccaggccg ggttcgccgc cgccacgatc
    13981 tgcgccagac gcgccgtggt ctcgatcggc tcgacgctgc gctcgcgcac gatcgccgcg
    14041 gccatgcggc gcgcgaagcg ctcttcaccg tactcgaaca gcacctgcgc gatatcctcg
    14101 gcccgggcac gcgcgagcca ctgggccgcg ctgatgccac gcccggggtt catgcgcatg
    14161 tcgaggggac cgtcctgcag gaagctgaag ccgcggcggg ggtcgtcgag ctgcggcgag
    14221 gacacgccga gatcgagcag cacgccgtgc acgcgtcccg ccatgtcgtg gcgctccagc
    14281 agcgtctgca gctcgtcgaa ggaagcatgc tcgatgacca ggcgcggatc gtcgctgaac
    14341 tggctgcgcc cggcggcaat cgcttccggg tcgtgatcga tggcgagcag cctggcggcg
    14401 ggggcgaggc gtcccaggat cgcgcgggtg tgcccgccgc gaccgaaggt gccgtctaca
    14461 tagctgccag cggaatctgt caccagaaaa tccaccgcct cctgcaacat gacaggtgtg
    14521 tgcattgcgc tgcgcccgcg tgcccgtggt ttacaaggaa agccgcagca tctcgtcggg
    14581 gactgcaccc tcgacgcttg ccatctgcag ccacttgtcg cgctcagcca gccacgtgtc
    14641 ctcgtcccac aactccatct tgttgccctg gccgaccagc acggccttct tgtccagccc
    14701 ggcgtattcg cgcagggccg gcggcaccag cacacggccg ctgccgtcgc attcgaggtc
    14761 gctcgcgtag ccgatcacga gacgctggaa tcggcgcacc acgggattga gggtcggcag
    14821 ggcctggatt tcgcgctcca ggtcctccca ggtcggcagg gggtagacca gcaggcactt
    14881 gctctgggtg tctatcgtgg ccacgagttg cccggcgctg atcgcctgca gccgatcacg
    14941 atgacgcgcc ggcatggcca tgcgcccctt cggatcgatg ctcagatggt ggacaccgcg
    15001 gaacactgtc agtttttacc ttgttcggtc ccaggaacgc ccatgctcgc cactttcgcc
    15061 cactttcacc cacttttcga cactatatgg acgcacgact tccggtgtca aggacgccag
    15121 gaccatcaag tgacgaattt tcttgttgaa aaaccggtac ttaggtgaac agagcggagg
    15181 cgaggacagg ctgccacgga gcttgctgac agataacatg ccattgcgca agcaagttaa
    15241 agtgatctct catgaatgag aatattttgt tcttgttgga tgtttcttat attcaaatag
    15301 gaataaagaa agttgcagag tcggcctgta agccgggttc tgtcgaggac agtcattcat
    15361 ctgggacgtg cgtcaccgca cgcctctagc gacctacccg aaccctgtgc gggccgcact
    15421 gatgggttcc tatttggtct tgctccgggt ggggtttacc gtgccacgcc tgttaccagc
    15481 cgcgcggtgc gctcttaccg caccctttca cccttacctg ccaactgcat ccggagatgc
    15541 ggccgacatc ggcggtttgc tttctgttgc actttccgtg ggctcgcgcc ccccaggcgt
    15601 tacctggcac catgccctat ggagcccgga ctttcctccc ggtcactcac cacaggcagg
    15661 ccgcttgcgc agctgcgaca tgcctctgga gagtgaccga gcgactgccc ggccgactct
    15721 gcgcgcggga gtatatcacc cggcgtcgcc gccgggccgc agggcgagcg cggcgcgata
    15781 gacttcgttg cgcggacgtc cgctgatctc cacggcacag gcgaccgcct ccttgagcgg
    15841 caggcgcttg agcagcgcct cgagcagcgc accggtactg atcgcggttt cgtccgcgac
    15901 aacggcctcg gcgccggcga gcaccagcac gatctcgccc agctgctgct ccggatcggc
    15961 cgcgacccgc tcgagcacct cgttcaccgt cccgctcagc agtgtctcgt gcaccttggt
    16021 gagttctcgc gccagcgcgc aacggcgctc gccaccgaac accgccgcga ggtcgcgcag
    16081 cgtgtcgagg atgcgccgcg gcgattcgta gaagatcagc gtacgcgttt ccatgccgag
    16141 cccttcgagc cgggcttgcc gcgccgcctg gcgggcaggc aggaaaccct cgaagacgaa
    16201 acggtccgag ggcaggcctg ccacgctgag cgccgccacc gccgcgcagg gacccggcac
    16261 cgccacgacg ctgattccgg ccgcgagcgc gtcgtgcacc aggcggtagc ccggatcgga
    16321 aatcagcggc gtgcccgcat cggacaccag cgcaaccgaa ctgccgtcac gcagcagttg
    16381 aagcacgtgc tcgagccggc gcgcatcgct gtggtcgtgg taggccacca cggggcgcga
    16441 gatgcccagg tggttcagca actgccggca gcgccgggta tcctcggcgg cgatgaggtc
    16501 tgccgcggcc agcacttgcg cggcgcggtg cgaaagatcg ccgagatttc cgatcggggt
    16561 ggcaacaacg aacagcactg cgggcgtctc cacggcggca tccggcgccc ctgccggcgc
    16621 gggcgccggc aggggcgccg gcgcatcggt ttgcgctagt atacgggcat gaacagagcc
    16681 ttcgtccgtc cgctgctcgt cgccgcgctg ctggttgccg cagggtgctc cgatcagccc
    16741 gaaaagccgt cttccgagca catcccgcta ccagagaagc ccgcgaccca gccggctgaa
    16801 cccgcggacg aggtggagcg gctgctcgcg ctggcggcgc gcagtgacgg cgctgccgca
    16861 cgcgaggcga gactcgatgc agcggccgaa ctcgtgcgcc gcggcgacct cgcccgcgcc
    16921 gcaacgctgc tggacgaact cgaggcggca agcctcgaca gcgcgcaatg gattcgccat
    16981 tcgctgctgc gcgcgcgtct cgagctcgag ggaggcgacg cggcggcggc tctcgcgcgc
    17041 ctcgacaatc ccgcgctgca ggccgaaggc gccgcggtgg ggcgcgcgtt gcaggtcgag
    17101 ctcgccctca cgcgcgccga cacactcgcc gcgctgcaac agcacctgga gagcgcccgg
    17161 gaacgcgtgc gggtgcagcc cctgctcagc gacgagacag cgcgccatga caacacccac
    17221 gcgatcttcg cggcgctggc cggcgcggac atggccgcgc tggaacgcga gaccggcgtc
    17281 gcggcgagcg atgactggcg cggctggctg gagctcacgg cgctcgtacg cgacctgcgc
    17341 cgcggccccg atgagcagct tggcgcgctg gcaagctggg aacagcgcta ctccctgatc
    17401 gcccccctgc aaccggcggt gaacgaacta ctgcccgcca tccgtgaacg tgtccgccag
    17461 cctgcccgcg tggcgctgtt gctgccgttg agcggccgcg cggcgcccgg cgcgacagca
    17521 gtgctgaacg gctacctcgc cgagcacatg cggcagctcg cagcgggcga gcagccggtg
    17581 cccgttgcgg tggtcgatac cgccgccgcg cccgggggct tcgcggccgc atatgccagc
    17641 gcggtagccg acggcgccga catggtcatc ggtccgttgc tcaaggagga cctcgcggca
    17701 ttcggcccca cgctggcacc ctcggtgacc acgatcgcgc tcaactttgc cgatagcgcg
    17761 aacagcgacg gcgacatgct ctacgagttc ggcctcgacg gcgccgacga ggttgcgcaa
    17821 ctcgcgacag cggcgcgggc gcgcggttgg gcgcatgcca tcgtggtagc agacgattcc
    17881 gatacctcac ggcgccaggc gcaggagttc gagcgcctgt ggaacggcgg cgacacgcgg
    17941 gtgctgggta cactgtatct tgcagacatc aatgaattcc gccgccgctt cgaggaggcc
    18001 ctgctgttcg aacagagccg ccagcgcagc gcggcactgt cacgactgct gggactggcg
    18061 ctcgtgtcgg aaccgcggcg gcgccaggac ctcgacctca tcgtcatgtt ctcgggaccg
    18121 gtcggcgcgc gctccatccg gccgctgctg tcgttcctgt atgccggcga cctgcccgtg
    18181 tggtcgacca gccaggccaa cggcgcgcgt cccgaccggc gcaacgaccg cgacctggag
    18241 tacgtgcgct tcctcgacct gccgtggttc tccggggccg aacgcccgtt gcgcgaaacg
    18301 ctgcgccgca gcgtaccggc cggcggcctg gaacgctttg ctgcccttgg cgccgacgcc
    18361 tgccgcctgc agtctcgcat cggtttgctg gactggaacg gcgcggccgg cctcggcggg
    18421 gccaccggtg aactggtact ggacggcacg cgacggctgc accgcaaatc acagtggtac
    18481 gtgttccgcg acggcctcgc cgcaccggaa atacagcgta ccgcgctgct cgccacaccc
    18541 ctgaaccgtc aatccacgga aggagcgaca ccatggaccg acaccaccag cgcggcacct
    18601 ccgccgccac cggacgcgcc gcagaagacc gcgccctgaa ggaactcggc gcggcggggt
    18661 tgcgcctcgt cgcacgcaat ttccgctgcc gcctcggcga gatcgatctc gtcatgcgcg
    18721 agggcgacac gctggtattc gtcgaggtgc gacagcggca gagcgcgcgt ttcggcgacg
    18781 ccagcgagag cgtcaccgtc gcgaagcagc gcaagctact ggctgccgcg gcattgttcg
    18841 tggcatggca tccggcgctg cgcgacctgc cgatgcgatt cgacctgttc gcgctggacg
    18901 ggaacgcgca cggatcgacc acggtgcccg gcgcaaaatg gctgcgcggc atcatcagct
    18961 gaaagcggaa ggaggagccg ttgtctctcg atatgctcaa tgagccgctg caggcgctgt
    19021 tcgccagcca cgtcgatacc gtgtaccgcg cgctcgacgt catgtacccg gagattcacc
    19081 gggcggtcga actgctctgc gacagcctgc tggcagaccg ccgcgtgctc gtctgcggca
    19141 gcggaaccgg caccgcgctg ggccaggtgt tctgcaccaa cctgctgaat cgcgcccaga
    19201 tcgaccgccc ggggctcccg gtgatcgcga tcggcagcga tccggccgcc accagcgcga
    19261 tcgccgagag ctacggcatc agcgaggtgc agtcgcgcca gatccgcgcc ctgggccagg
    19321 ccggcgatgc cctgatggtg atcgcgggat cgccgcgcgg tgcgggcctg agcccggcgg
    19381 tgcgcgctgc ccacgcgcgc gagatgcgcg tcatcgccct cacgggcggc gaaggcggag
    19441 aaccggcggc cgccctcgga ccggcagaca tcgagctgcg cgtgccctcg gatgatccct
    19501 accgcgtcag cgaatcccag ctgctgctgc tgaacgccct gagcgcgctg atcgaactgc
    19561 gcctgttcgg cgaggcgtga gggctcactt caccacgcgc agcgacggct tgccgctccg
    19621 gggctcgtcg tccgacgacg gcggtggcgc ctgctcgggc tcgaacacca tgccctgccc
    19681 gttctcgcgc gcgtagattc ccagcacggc gccgatcggc acgaagacat cgatcgccac
    19741 gccgccgaag cgtccgcgga accggatgtc gtcgttggac atgtcgatcc cggccaccgc
    19801 cccgggcgcc acgttgagca cgatctgccc gttcttgacg tactgctgcg gcacctcgac
    19861 gcccgcaacc gtcgcatcca cgagcaggta gggcgtgcat gcgttgtcga ggatccactc
    19921 atagagtgcg cgcaccaggt agggtcgact ggatttcatg ctcatatcat ggcgcctgcc
    19981 gccgcccgcg cgggcggatc agcgtcggcg aatctccttt tcagtctcgg acaggctcgc
    20041 gcggaagcca tcgcgcgcga acatccgggt catgtaatcg tgcagcggcc tggtctgccg
    20101 gatgttgggc aggacgatgc ccagcgccgg caagcgccag agtattgcgc aaagacagca
    20161 atcggccagc gagaattcct cgctcatgaa aaacggcatt tccgcgaata ccggcgctac
    20221 ggccatcagc tcgtcgccga cttcacgccg cgccttcgcc accgcttgcg gcgggctcgc
    20281 cgggctctcc atcacatcga cccgcgaact ccagtcccgc tcgaggcggt acatgagctg
    20341 gcggcactcg ccgcgcgcaa ccgggtagac cggcagcagc gggggatgcg ggaagcgttc
    20401 gtcgagatat tccatcatga tcccgggctg gtagagcacg agttcacgat cgacgagcgt
    20461 cggcaggctg ctgtagggat tgatctcggc gagagccggg ggataatcac ggccatcgac
    20521 atcgataagc tccgccgcta cccccttctc ggccagcacg atgcgaaccc ggtggctgta
    20581 atggcatcgc gcatcggaga acagcgtcat cgccgtgcgc ctggccagta cggccctgat
    20641 gtctctgccg gggcgcatgg ggcaagggat cagcgccgcc gtgccgggca cggcggcagc
    20701 gcggcctcag tggacgtctt tccagtattc ccggttcagc aggtacgcga ataccccgaa
    20761 gaaggccagg aacagcagca cgtagatgcc catgcgcttg cgctccagcg ccatcggctc
    20821 ggccacatag gcgaggaagc tcgccaggtc cgccatagcg gtgtcgaatt cctcgaccgt
    20881 catgctgccg gcctgcgcga ccttgagacg tccgcacgga gcttccagca ggtcttcacc
    20941 ggtcagcggg tcacgcttga tgccaccgtt gtgcgccagc accgggccgg gcgcgcactc
    21001 gggcaggccc tgcagctcga tcagcacgtg cggcatcgcg acgtcaggga acacgcggtt
    21061 gttcacgccc cacggacgtg ccggcgaatc atcgcggtag aagttgcgca ggtaggtata
    21121 gatgtactct ggcgagcgcg cgcgcgagat cagcgtcagg tcgggcggcg gcgcgccgaa
    21181 ccacttcttc gccagcggca cgggcatcgc gttctccatc agctcgccga tcttggcatc
    21241 gtcgaagatc aggttctgct ctgccagcgg cagcggaatg cccaggtccg ttgcggttcg
    21301 ctcgaagcgc gcgtacttca gcgaatggca gcccatgcag tagttcatgt agtacttcgc
    21361 gccgcgctgc agcgaggcct tgtcgttcag ggccggcgag aactcgtcgc agggaatcgt
    21421 gccgcagtcg aacgccgctt ccgccccgac cgccttcagc ggcaggaagg ccatgagccc
    21481 caccagcacg agcaccgcca ggaacttcca cggccccatg ccaccatcca tggtcacgcg
    21541 ctccggcacg ggcctggtct tctcgatcga ggtgtagacc ggcatcagca ggaagaacgc
    21601 gaaatagagc accgtgcaga tctgcgccag gaaggtacgc tccggggtcg gcgccttcac
    21661 gccgagcacg ccgaggatga tgaacgaggc cgcgaagatc acgatcgccg cacggctcag
    21721 cgcacccttg tagcggatgg acttcaccgg gctgcggtcc agccagggca gcacgaacat
    21781 gatcgcgatc gccgccgcca tcaccacgaa gccgccgaac ttgtccggca cggcgcgcag
    21841 catcgagtag aagggcgtgt agtaccacac cggcgcgatg tgcgcaggcg tcttcagcga
    21901 gttggcttcc tcgaagttgg cgaactccag gaagtagccg cccatctcgg gcgcgaagaa
    21961 gatgatcgcg cagaacacga acaggaacac caccacgccg acgatgtcat gcacggtgta
    22021 gtaggggtgg aaggcaacgc cgtcgagcgg cacgccgttg gcgtccttct tcttcttgat
    22081 ctcgacgccg tcggggttgt t
//
LOCUS       DAORUQ010000028        21861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1978_length_21861_cov_6.690131, whole genome shotgun sequence.
ACCESSION   DAORUQ010000028 DAORUQ010000000
VERSION     DAORUQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21861
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_21861_cov_6.690131"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="rfbD"
                     /locus_tag="PLW13_04350"
     CDS             complement(<1..660)
                     /gene="rfbD"
                     /locus_tag="PLW13_04350"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQY69631.1"
                     /translation="MKVLVTGVNGQVGWELARRVPAGVTLDASDRAVLDLASDDVAAR
                     VIARRPDVIINAGAYTAVDRAESESVLAHRVNADGAGALARAARELGARLVHVSTDFV
                     FDGSASRPYAPDCAPAPLGAYGASKLAGECRVLEESAGRSVIVRTAWVYSAHGNNFVK
                     TMLRLMRERERVAVVADQVGTPTWAGLLAGALWELALRPEVQGILHWTDLGVASWYDF
                     AV"
     gene            809..2323
                     /locus_tag="PLW13_04355"
     CDS             809..2323
                     /locus_tag="PLW13_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69632.1"
                     /translation="MPGSRSENQHHLYLLLISASCALSALAILRDPILDDVGVLHLLL
                     AREAADTGIGAALALFDRPIYPILIAGLHQLSGLSLLASAHLLGAALLALLVLGFTRF
                     AVLLCGERELAPWIALLVLLFPLLNEYRSLVLRDFGFWALLVCSLLALLRYQATLRWR
                     HGLWWALLGAASAAFRPEALVYMALLPLSCLQGETPATRLMNAARLHACMGLLALPPL
                     LVLARFDMLQAPLDALAGTLESVAATIAEGFAGASARYADTVLDPRVGALAPLALAAG
                     LLTVLALKFAAALGPVYCVLLGWGVAAGRAGLPSVARRTWRVLGATALLIVVTVVLAR
                     QFVAERHLLMPCLLALIPAALALRSFAETARRERRQGAFHLVLGIAIVLLVADGLVRF
                     GTRHDYRHQAIAWMQDELPAAARVFSNDRTLAYYSGGRFDRDAVSQGDALIVAQRAPL
                     AGVEYWIVRREERNPALDAALAAYLPRLTPMARFDNGHDARIEVFRVTAGAGPQ"
     assembly_gap    2349..2448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2449..3986
                     /locus_tag="PLW13_04360"
     CDS             <2449..3986
                     /locus_tag="PLW13_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69633.1"
                     /translation="RHRRRRPAMSASTTGLSRFYLLLAALTLALFAFANLRSPLVNED
                     GVLYLLLAERIGNEGLSSAFALYDRPFYPWLISAIHAVSGLSLTHSALCLDAAFCVLL
                     TLAFVEFCRLLYDDRGVLPWAALLILAHPKLNNYFGFVIRDIGYWALLFVSFCAWLRH
                     MRDARMRWLLVWAPATLLAAAQRPEALVFGLLLPPMLLLAPRGARGASLARVLLAWGL
                     VFGAMALALALVAALDPATFVQPMRDANDVPAALWRDIPTHFRAAAERYAHEVLDPHS
                     GDVAALSLAGGLLTILLVKILNGLGPLQCLLLAHGLWRSSVAVAAMHRRAYATMLVGA
                     VLIAAAFVGYRHFLDTRYVMLAAMLMLAPAARSLQKLAAHAFARGASARAAFVAAALL
                     LAGLDLLLGLDRPKPYLGECALWLRANLPPGSPVFTNDKQLAWTSGARWDYQTTHDAN
                     LLIAEGSVPLAGNAYWIIHLKRGNEELSRQLEHYRPLLTPLHRCADPRGSVVEISRQA
                     PAP"
     gene            complement(4037..4966)
                     /locus_tag="PLW13_04365"
     CDS             complement(4037..4966)
                     /locus_tag="PLW13_04365"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HQY69634.1"
                     /translation="MNFDDRDGVIWFDGKMVPWREARTHVMTHTLHYGLGVFEGVRAY
                     HTAERGTCIFRLQEHTNRLFGSAHILRMDMPFDKDTINEAHRAVVRENGLKEAYLRPL
                     AYLGSEAMGLRAEGLKTHVVVAAWEWPSYMDPEARERGIKVRTSSYTRHHVNITMCKA
                     KAVGNYTNSMLALREAIESGAEEALLLDNEGYVAEGSGENFFMVRGGRIHTPELTSCL
                     EGITRDTIIRLARDMGLELVERRITRDEVYLADEAFFTGTAAEVVPIREVDGRSVGTG
                     RRGPVTTELQARYFDLVRGKRADYPEWLAPVAG"
     gene            complement(4963..7902)
                     /gene="glnE"
                     /locus_tag="PLW13_04370"
     CDS             complement(4963..7902)
                     /gene="glnE"
                     /locus_tag="PLW13_04370"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HQY69635.1"
                     /translation="MNTLPLEHVPLPLHAALERDWQEILAALEPAAREAVQARLAADA
                     ALAAALARVLGCSQFVAQAFARDSELLPGLLESGDLARSYDDCEFRQRLALALPADAD
                     EARLATELRRLRRREMVRIVWRDLLRAADLVETTRDLTWLAEATVEAAVAHGHAQLAA
                     RHGEPRGRESGAPQRLVVLGMGKLGACELNLSSDIDLIFAYPEQGETDGERGLSNQEF
                     FTRLGQRVIKAIDAKTQDGFVFRVDMRLRPYGDAGALALNFDAMEEYYQDQGRDWERY
                     AMIKARVIAGDRAAGEQLLAALRPFTYRRYIDFSAIESLRGMKAMINREVQRRGKTTD
                     VKLGAGGIREIEFIAQSFQLIRGGRETALQERRLLVVLELLGSLGHLPAAVVAQLAEA
                     YVFLRNTEHAIQARADQQTQALPEDEHDRLRLAFALGLPDWSAFTAELERHRAHVRRH
                     FADVVAAPQEQHAAEQAAPDAWRALWAMEKDPERLLELLEATGFEDPADTLKRLGALR
                     NSNRLQALQSAGRERLERFMPLLLAAAAATEHPSAVIARTMALVESVLRRSAYLVLLE
                     ENPGALEQLVRLCSASPWIAQELANHPVLLDELIDPRALYTLPERATLAAELQQHMLR
                     IPLDDLEAQMEALRYFKLAHRLRCAASEVTGALPLMKVSDYLSFLAETILGHVLAVAW
                     HYLVERHGTPQKAGGVPCDPDFIIVAYGKLGGIELAHGSDLDLVFIHDADPMLATDGE
                     RAIENGVFFTRLGQRIIHVLTAYTPLGQLYEVDMRLRPSGAAGLLVSSLTAFREYQYH
                     QAWTWEHQALVRARPVAGCSRLAARFEELRREVLAQPRELPRLRRDVIEMRDKMRAHL
                     LGPETSGGKSPRFDLKQSVGGIVDIEFLVQYCVLAWSENQAALSVYTDNIRILEALSE
                     SGLMASRDAQVLTEAYKAYRTVVHRLTLQQQDDIVPALEYQALRDEVVRVWDALLGSE
                     PTGENNT"
     gene            complement(7899..9692)
                     /locus_tag="PLW13_04375"
     CDS             complement(7899..9692)
                     /locus_tag="PLW13_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQY69636.1"
                     /translation="MSEPQTLSAGKPIDQGELLDALAADGLIDAADARRLRYAPRTRE
                     GVARHPVNFVADQQLVAPADARPLGVERLLGWLAQRTGQAVYHIDPLRIDAAAVTAVM
                     SFAFAKRHRILAVESTAAEVVIAGAEPLVSAWEPDLARTLKRPIRRVIADPADIERYA
                     VEFYSLAKSVSGASGARGVQTTAGLANFEQLVELGKLKDPDANDQHIVSIVDWLLQYA
                     FDQRASDIHLEPRRELANVRFRIDGILHGVYQLPTPVAMAVTSRLKILGRMDLAEKRR
                     PQDGRVKSRTPAGSEIELRLSTLPTAFGEKLVMRIFDPEVLVRSFADLGLADEDHAQW
                     RGMVEQPHGIVLVTGPTGSGKTTTLYSTLKQLATAEVNVSTIEDPIEMVEPAFNQVQV
                     QHGIGLDFASGVRALLRQDPDIIMIGEIRDRETAEMAVQAALTGHLVLSTLHTNDSPS
                     AITRLLELGIPSYLIRATVLGVMAQRLLRILCPHCKVPAAIDEDVWSRLVAPWKAKPP
                     ATVYAANGCLECRNTGFSGRQGIYEILRVDTAVQRAVREGCDSEELRLIGMRAGMKTL
                     RLAGARKIAAGLTTLEEVLRVAPMDNSRDGA"
     gene            complement(9784..11064)
                     /locus_tag="PLW13_04380"
     CDS             complement(9784..11064)
                     /locus_tag="PLW13_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQY69637.1"
                     /translation="MTASKPHHSQTDLFDASPRPPPAQELRIAPQGAAPLGKEQQTFN
                     RLVRKVEILREKLAEWAAFEPQHHRCVAKDLMPVLKQLGAARRETILTLARILEGKAA
                     GGRPGKVEQRNLVHHLLELARAQLEDDPQDEQIIALHDQYADIGFAEDQELDRELARG
                     MAEQMFGVELEEHELGSSVEEAMAAAAAKARQAAEEEAQAARARRAAGAKARTRATDK
                     AAAAEQAAKEASQSVREVFRKLASALHPDRAGDEVERARRHELMQRVNHAYDEGDLLT
                     LLTLQLETEQIDAAHLARVSTARLGHYNKVLREQLRELEQELAGITGHFAGLTGQAPA
                     VVTPASVQHALKRELAELKRAVKALKAHHAMMQEPAQRKRWLQQYARDLREEERLDGL
                     LDAFLDAPDAFVDMDFDPFRGPGSAPRRGRKRRR"
     gene            11183..14707
                     /locus_tag="PLW13_04385"
     CDS             11183..14707
                     /locus_tag="PLW13_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HQY69638.1"
                     /translation="MTEQIDTAVEDFIVRWEGAGGSERANYQLFMNELTVLLAVEQPH
                     PQAQDARDHAYVYERRVTFRHGDGSESNGYIDCYKRACFVGEAKRLKAAPDTRGFDDA
                     MLRARSQAEQYARALPADEGRPPFLVVFDVGRRIDLYSDFTRSGATYVPFPDPRSHRI
                     ALADLRRPDIRDRLRAVWTEPLSLDPAQAAARVTREIAARLAELAKSLERAGHPAQPV
                     AQFLMRCLFTMFAEDVRLLPPNSFRDLLDRYREQPDTAMRMLEQLWRDMDRGGFSPVL
                     AVDVLRFSGKLFRAPDTLPLDRDQIGLLHAAARADWRLVEPAIFGTLLERALDPAERH
                     ALGAHFTPRAYVERLVLPTVIEPLRAEWIEAQAAALTVSGESRDAEAVAILRAFHHRL
                     CTVRVLDPACGSGNFLYVTLEHLKRLEGEVLNALDELGASQVSLALDHERADASAGET
                     VDPHQLLGIELNPRAAAIAEVVLWIGYLQWHFRTHGQVNPPQPVIRDFRNIENRDAVL
                     AYDRVEYLSDEGGVPVTRWDGTTYKTSPVTGEQIPDESARIPVEHYVNPRKAEWPEAD
                     FVVGNPPFIGNKRMRIALGDGYVEALREVWAAVPDSADFVMYWWESAAQLAREGSISR
                     FGLITTNSLRQAFNRRVLELHLGAKENPLSLTFAIPDHPWVDSVEGAAVRIAMTVGRQ
                     AAASGVNQTDGVLLNCEQEVSGDAGEYIVRFRRSDGIINADLSIGSDLTRASRLAANR
                     GLACPGVQLSGQGFVLLPEDLHRFSITARQRVIRPYITGRDITQRPRAQFVIDTFGLA
                     ELELRSAYPDLYQHLANTVLPDRLGVSTGSKDVAKYAKEWWLHSKPRPKFRPALSGLD
                     QFIVTSRTAKHRIFVFAEADTLPETKVLIVATGDAAYLSMLSSAVHLLFADCAGGRHG
                     VGNDLTYNHSDCFEKFPFPILSDTQTTTLRTLGEQLDAHRKRQQAAHPALTLTGMYNV
                     LEKLRSGDTLSDKERAIHEQGLVSVLRQLHDEIDAAVLDAYGWSDLLPLLRIAHGNTP
                     PAADQTREDAGRAFDAAVLERLVALNTERAAEEARGLVRWLRPEFQNPQASAAPEQEA
                     MDVAAADEDIAAPVHATKPVPWPKDTLQQVRAVAEAIAASAAGLTLAEIEARFTARGP
                     WKRRLPIVLDTLAAVGRVREHEGRYRAM"
     gene            14817..16214
                     /locus_tag="PLW13_04390"
     CDS             14817..16214
                     /locus_tag="PLW13_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HQY69639.1"
                     /translation="METCTPSPCSRGAGLDPESLSAAALEDEIHTLSASITVATWRLL
                     MLIAELDRRRAWQHWGVISCAHWLNWKCGIDLGAAREKVRVARALENLPLVNDALARG
                     ELSYSKARAITRVATPESQDYLLMIARHGTASHVEKLVRAYRHVERLQVSARARSTHA
                     QRALRWRFEDDGSMVITVRLPVEQGALVVQAIHAAVDAQWRERDGADREQELADDVSA
                     ETSPASSPSSNSFDNAESPVSARRADALCHVAEQFLAHEAVTSRQAERYQVVVHVDRA
                     VLSEHGHGDCCHIEHGSAIAAETARRLVCDASVVTLEESGDGEPLNIGRRSRTIPPAI
                     RRALAHRDKGCRFPGCLNHLYVDAHHIRHWADGGETRLDNLVLACRHHHGLLHEGGFT
                     VARRDDGKLLFFRPDGELLPEVPKPPLRVHDIEAIVAESGIDVSAETSRSLWDGSRMD
                     LDMAVGGLMTLNGWR"
     gene            16708..17337
                     /locus_tag="PLW13_04395"
     CDS             16708..17337
                     /locus_tag="PLW13_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HQY69640.1"
                     /translation="MEYVNFGGYPELALSPTVRGNPERFVKSDIVDKVLLRDLPQLYG
                     IKDIQELNSLFTLLAFNTAEEVSFEQLSQRSGVGKQTIQRYIEYLEAAFLIKRVFRID
                     QDGKRYQRERNFKVYLTNSAMYTGLFGAKDPDEREFGHLVETALFEQRFHEDARLNYA
                     RWGAEDSEVDLVESSAALKPIAALAFHYGARAVQGRLAGYEAQIQGVTA"
     gene            17506..18669
                     /gene="argE"
                     /locus_tag="PLW13_04400"
     CDS             17506..18669
                     /gene="argE"
                     /locus_tag="PLW13_04400"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HQY69641.1"
                     /translation="MSRPVPDFLTMFDRLLASPSVSCTEPEWDEGNRGVCELLAEWLQ
                     ALGFRCEIQQMPSRADKVNLIAVLGEGPGGLVLAGHTDTVPFDEKLWEMNPFGLTVRD
                     ERLYGLGATDMKGFFPIAIEAALAFRGARLREPLILVATADEESSMDGANMLVATGKP
                     RARHAIIGEPTDLRPVRMHKGMMMEAVTVTGQSGHSSNPALGRNALEGMQHVMADLLA
                     FRDELAARHRNPLFEVEVPTLNFGCIHGGDNPNRICGECELHFDLRPLPGMDLEDLRA
                     VIRARLDGVAARTGLGITMRPLFPGLHPFAEDAAAPIVRAAEEFTGYSAGNVAFATES
                     PFLQRLGMQTVVMGPGSIRQAHQPNEYMEQFQVEPMVTVLQKMIQRFCVDGHS"
     gene            18672..19970
                     /gene="argA"
                     /locus_tag="PLW13_04405"
     CDS             18672..19970
                     /gene="argA"
                     /locus_tag="PLW13_04405"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HQY69642.1"
                     /translation="MSGSFPQFVNSFRNSAPYINAHKGKTFVLVCGGEVVADERFSSI
                     VHDIALLSTLGVNLVLVHGSRSQVEAGLQSANAASSVVHGTRVTDARALELAVQAAGM
                     VRARIEALLSMGIANSPMHNAGIRVCGGNFVTAQPLGVLGGVDYQHTGKVRRVDHEGI
                     RAQLALGSIVLLPPLGYSPTGEIFNLTKEDVAVSAATALRADKLILLEGAPLTDASRQ
                     PLRDLPLTLAQTVLAGNATPALAAAIQACQQGVERCHILDFHTDGALLHELFTRDGIG
                     AMINRDAYESIRIAGIEDVGGIMELIEPLERDGTLVRRSRERLELEIACFSVVERDGA
                     IIGCAALYPDLPNRTGELACVATDPVYRRSGRANRLLEHIEGRARAEGIDRLFVLTTR
                     TAHWFVERGFRPAELAELPEKRRELYNYQRNSLVLIKDLS"
     gene            20175..20435
                     /locus_tag="PLW13_04410"
     CDS             20175..20435
                     /locus_tag="PLW13_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY69643.1"
                     /translation="MTTIRSPRQLGDALRAARKQLGLTQPQLALAAGVGVRFIVDLEA
                     GKPTLRLQNVLRVIDALGGEIQLRDLPSVVADDQREGGDHGA"
     gene            20425..21720
                     /locus_tag="PLW13_04415"
     CDS             20425..21720
                     /locus_tag="PLW13_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HQY69644.1"
                     /translation="MAHELDVWLFADRVGTLALVDGRLDFCYASGWLSRPDAVALSAS
                     LPLQAEPFDDRKARPFFAGLLPEGQMRRLIAQQFQVSGQNDFALLEHIGGECAGAVTF
                     LEPGQALPVSTRNDDVQWLSDEEVVAILDELPRRPMLAGKDGLRLSLAGAQDKLPVVF
                     DGARIGLPLNGTPSSHILKPAIQAVEDSVINEGFCMALADAMQLKPAKSKVHRVLARS
                     FLLVERYDRPIDAQGYWQRLHQEDFCQALGVAPEMKYQNEGGPDLARCFDLVRRATRP
                     NAPQILRLLDYVIFNALIGNHDAHAKNFSLLYSGKSPVLAPFYDTLSTAAYPTLTPKM
                     AMKIGSKYKFSEVRARHWEQFAESVGLTKAQARRRILELAKSLPTTARKLQSDPGRGF
                     AGNALVERINALIEQRCALTIRRLTDPAAEKEADANPSA"
BASE COUNT         3342 a         7470 c         7388 g         3561 t
ORIGIN      
        1 caccgcgaag tcgtaccagc tggcaacgcc gagatcggtc cagtgcagta tgccctgcac
       61 ttcgggcctc agcgcgagct cccacagggc gccggccagc aacccggccc aggtgggcgt
      121 gccaacctgg tcggcgacca ccgccacgcg ctcgcgctcg cgcatgaggc gcagcattgt
      181 cttcacgaag ttgttgccgt gcgcggaata gacccacgcg gtgcgcacga tcacgctgcg
      241 gcccgcgctt tcttccagca cgcggcactc cccggcaagc ttgctggcgc cgtaggcccc
      301 gagcggcgcg ggcgcgcagt ccggcgcata gggtcgcgaa gcgctgccgt cgaagacgaa
      361 atcggtcgac acgtgcacca gccgggcgcc gagctcgcgc gcggcgcgcg cgagggctcc
      421 ggccccgtcg gcgttgacgc gatgcgcaag cacggactcg ctctcggcgc gatcgaccgc
      481 ggtataggcc ccggcgttga tgatcacgtc ggggcggcgc gcgatcacgc gcgcggcgac
      541 atcgtcggag gcgagatcga gcaccgcgcg atcgctggcg tccagggtga caccggcagg
      601 cacgcggcgc gcgagctccc agcccacctg tccgttcact ccggtcacca gcactttcat
      661 cgtccgacgc gcccccccga aaggcggcgg cgatgttagc acaggcgcgc cgccgggccg
      721 ggacccggtg cacgcggcgg ctgacttgga gggcagcctt gctgcatact gtcgcgcgcc
      781 tttgcgacat gcggattcga cctgccagat gccgggttca cggagcgaga accaacacca
      841 cctgtatctc ctgctgatct cggcaagctg cgcgctgtcg gcgctggcga tactgcgcga
      901 cccgatcctc gacgacgtcg gcgtgctcca tctcctgctg gcgcgcgagg ccgcggacac
      961 gggcatcggc gctgcgctcg cgctgttcga ccgcccgatc tacccgatcc tgatcgccgg
     1021 cctgcaccag ctgagcgggc tctcgctgct cgccagcgcc cacctgctcg gcgccgcgct
     1081 gctcgcgttg ctggtgctgg gcttcacgcg tttcgccgtt ctgctgtgcg gagagcgtga
     1141 actcgcgccg tggatcgcgc tgctggtgct gctgttcccg ctgctgaacg aataccgcag
     1201 cctggtgctg cgcgacttcg gcttctgggc gctgctggtg tgctcgctgc tggcgctgct
     1261 gcgctaccag gccacgctgc gctggcgcca cggcctgtgg tgggcgctgc tcggtgccgc
     1321 gtccgcggcg ttccggcccg aggcgctggt ctacatggcg ctgctgccgc tttcctgcct
     1381 gcagggcgaa acgccggcca cgcgcctcat gaacgccgcg cgcctccacg cctgcatggg
     1441 gctgctggcg ctgcccccgc tgctggtgct ggcccgtttc gacatgctgc aggcgccgct
     1501 cgacgctctc gcgggcacgc tggagtcagt ggccgccacc atcgccgagg gcttcgccgg
     1561 cgcctcggcg cgctacgccg acaccgtgct cgaccctcgc gtcggcgctc tcgcaccgct
     1621 agcactggcc gcggggctgc tgacggtgct ggcgctcaaa ttcgccgcgg cgctcggccc
     1681 ggtctactgc gtgctgctcg gctggggcgt ggcggccggg cgcgccggcc tgccgtcggt
     1741 cgcgcgccgg acatggcgcg tgctcggcgc cacggcgctg ctgatcgtgg tgaccgtcgt
     1801 gctggcgcgc cagttcgtgg ccgagcgtca cctgctgatg ccgtgcctgc tggcgctgat
     1861 cccggcggcg ctggcgctgc gctccttcgc cgagaccgcg cgccgcgaac ggcgccaggg
     1921 cgcgttccat ctcgtgctgg gcatcgctat cgtgctgctc gtggccgacg ggctggtgcg
     1981 ctttggcacc cgccatgact accgccacca ggccatcgcg tggatgcagg acgagttgcc
     2041 cgcggctgcg cgcgtattca gcaacgaccg taccctcgcg tactacagcg gggggcgctt
     2101 cgaccgcgac gcggtgtcgc agggcgatgc gctgatcgtc gcgcaacgtg cgccgctcgc
     2161 gggcgtcgag tactggatcg tgcgccgcga ggagcgcaac cccgccctgg atgccgcact
     2221 cgcggcctac ctgccgcgcc tcacgccgat ggcgcgcttc gacaatggcc acgacgcacg
     2281 catcgaggtg tttcgcgtca ccgccggcgc cggcccgcaa tgagcgcgtc gacgacgggg
     2341 ctgtcgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cgtcaccgcc
     2461 ggcgccggcc cgcaatgagc gcgtcgacga cggggctgtc gcgcttctac ctgctgctcg
     2521 ccgcgctcac cctggcgctg ttcgcgttcg cgaacctgcg cagcccgctg gtcaacgagg
     2581 acggcgtgct ctacctgctg ctcgccgagc gcatcggcaa cgagggcctc tcctccgcgt
     2641 tcgcgctcta cgaccgcccc ttctatccct ggctgatcag cgcgatacac gcggtgtccg
     2701 gcctctcgct cacgcacagc gcgctgtgcc tggacgcggc gttctgcgtg ctgctgacgc
     2761 tggcgttcgt ggaattctgc cggctgctct acgacgatcg cggcgtgctg ccgtgggcgg
     2821 cgctgctgat cctcgctcac ccgaaactga acaactactt cggtttcgtc atccgcgaca
     2881 tcggctactg ggcgctgctg ttcgtgtcgt tctgcgcgtg gttgcgccac atgcgtgatg
     2941 cgcgcatgcg ctggctgctg gtatgggcgc cggccacact gctcgcggcc gcgcagcgcc
     3001 cggaggccct ggtgttcggc ctgctgctgc cgccgatgct gctgctcgcg ccgcgcggcg
     3061 cgcgcggcgc ctcgctcgcg cgcgtgctgc tcgcctgggg gctggtgttc ggtgccatgg
     3121 cgctcgctct ggcgttggtc gccgcactgg accccgccac gttcgtgcag cccatgcgcg
     3181 atgcgaacga cgtgcccgcg gcgctgtggc gcgacatccc gacgcacttc cgcgcggcgg
     3241 ccgagcgcta cgcgcacgag gtgctggatc cgcactcggg cgatgtcgcc gcgctctcgc
     3301 tggcgggcgg cctgctgacg atcctgctcg tgaagatcct gaacggtctc ggtccgctgc
     3361 aatgcctgct gctggcgcac ggactgtggc gctccagcgt cgctgtggcc gcgatgcatc
     3421 gtcgcgccta cgccacgatg ctcgtgggag ccgtgctgat cgcggccgcg ttcgtcggct
     3481 accgccactt cctcgacacg cgctacgtga tgctcgccgc catgctcatg ctggcgccgg
     3541 ccgcacgcag cctgcagaaa ctcgcggcgc acgcgttcgc gcgcggcgcc tcggcgcgcg
     3601 ccgccttcgt cgcggcagcg ctgctgctgg cgggcctgga cctgctgctg ggactggacc
     3661 ggccgaaacc ctacctgggc gagtgcgcgc tgtggctgcg cgcgaacctg cccccgggca
     3721 gcccggtatt caccaacgac aagcagctgg catggaccag cggcgcgcgc tgggactacc
     3781 agaccaccca cgatgccaac ctgctgatcg ccgagggcag cgtgccgctg gcgggcaacg
     3841 cgtactggat cattcacctg aaacgcggga acgaggaact ctcccggcag ctcgagcact
     3901 accggccgct gctcacgccg ctgcaccgct gcgcggaccc caggggaagc gtggtcgaga
     3961 tctccaggca ggcaccggcg ccctgatccg ggcgccgtgc cgtggccgcg ctggcggcca
     4021 cgcgggacgg ggaggatcag ccggcgacgg gtgccagcca ctccgggtaa tcggcgcgct
     4081 tgccgcgcac caggtcgaag tagcgcgcct gcagctcggt ggtgaccggg ccgcggcgcc
     4141 cggtgcccac gctgcggccg tcgacttcgc ggatcgggac gacttcggcg gcggtgccgg
     4201 tgaagaaggc ctcgtcggcg aggtagacct cgtcgcgcgt gatgcgccgc tccacgagct
     4261 cgaggcccat gtcgcgcgcg aggcggatga tcgtgtcgcg cgtgatgccc tcgaggcagg
     4321 acgtgagctc cggcgtgtgg atcctgccgc cgcgcaccat gaagaagttc tccccgctgc
     4381 cctcggccac gtagccctcg ttgtcgagca gcagcgcctc ctcggcaccg ctctcgatgg
     4441 cctcgcgcag cgcgagcatg gagttggtgt agttgccgac cgccttcgcc ttgcacatcg
     4501 tgatgttcac gtggtggcgc gtgtacgagg aggtgcgcac cttgatgccg cgctcgcgcg
     4561 cctcggggtc catgtacgac ggccattccc acgcggcaac gaccacgtgg gtcttcaggc
     4621 cctcggcgcg caggcccatg gcctcggaac cgagataggc gagcgggcgc aggtaggctt
     4681 ccttcaatcc gttctcgcgc accacggcgc gatgggcctc gttgatcgtg tccttgtcga
     4741 agggcatgtc catgcgcagg atgtgcgccg agccgaacag gcggttggtg tgctcctgca
     4801 ggcggaagat gcaggtgccg cgctcggcgg tgtggtaggc gcgcacgccc tcgaacacgc
     4861 ccagaccgta gtgcagcgtg tgcgtcatca cgtgcgtgcg cgcctcgcgc cagggaacca
     4921 tcttgccgtc gaaccagatc acgccgtcac gatcgtcgaa attcatgtgt tgttctcccc
     4981 ggtcggttca ctgcccagca acgcatccca tacccgcacg acctcatcgc gcagcgcctg
     5041 gtactcgagc gcgggcacga tgtcgtcctg ctgctgaagg gtgagtcgat ggaccacggt
     5101 gcgataggcc ttgtaggcct cggtgagcac ctgcgcgtcg cgcgacgcca tcaggccgct
     5161 ctcactcagt gcctcgagaa tgcggatgtt gtccgtatag acgctcaagg ccgcctggtt
     5221 ctccgaccag gccagcacgc agtattgaac cagaaattcg atatcgacga taccgccgac
     5281 actttgcttg agatcgaaac gcggactttt accgccgctg gtttctggcc cgagcaggtg
     5341 ggcgcgcatc ttgtcgcgca tctcgatcac gtcgcgacgc agccgcggca gttcacgcgg
     5401 ctgcgcgagc acctcgcggc gcagctcctc gaagcgcgcc gcgagccgcg aacagcccgc
     5461 gacggggcgc gcgcgcacca gcgcctggtg ctcccaggtc caggcctggt ggtactggta
     5521 ttcgcgaaag gccgtcagcg aggaaaccag cagaccggcg gcgcccgagg ggcgcaggcg
     5581 catgtcgacc tcgtagagct ggccgagcgg cgtgtaggcc gtgagcacgt ggatgatccg
     5641 ctgccccagg cgcgtgaaga acacgccgtt ctcgatggcg cgctcgccgt cggtcgcgag
     5701 catcgggtcg gcgtcgtgga tgaagaccag gtcgagatcc gaaccgtgcg cgagctcgat
     5761 accgcccagc ttgccgtagg ccacgatgat gaaatcgggg tcgcagggca cgccgcccgc
     5821 cttctgcggc gtgccatggc gctctaccag gtaatgccag gccaccgcga gcacgtgccc
     5881 gaggatggtc tcggcgagga agctcaggta gtcgctgacc ttcatcagcg gcagcgcgcc
     5941 ggtcacctcg gaggcggcgc agcgcagccg gtgggcgagc ttgaagtagc gcagcgcctc
     6001 catctgcgcc tcgagatcat cgagcgggat gcgcagcatg tgctgctgca gctcggcggc
     6061 cagcgtcgcg cgctcgggca gcgtgtagag cgcgcgcggg tcgatcagct cgtcgagcag
     6121 cacggggtgg ttcgccagct cctgcgcgat ccaggggctc gcgctgcaca atcgcaccag
     6181 ttgctccagg gcgccggggt tttcttccag cagcaccaaa taggcgctgc ggcgcagcac
     6241 cgactccacc agcgccatgg tgcgggcgat caccgcggac ggatgctccg tggccgcggc
     6301 cgccgccagc agcagcggca tgaagcgctc gagccgctcg cggcccgcgc tctgcagcgc
     6361 ctgcaggcga ttggagttgc gcagcgcacc caggcgcttg agcgtgtccg ccggatcctc
     6421 gaagccggtc gcctcgagca gttcgagcag ccgctccggg tccttctcca tcgcccacag
     6481 cgcgcgccag gcatcgggcg ctgcctgctc ggcagcgtgt tgctcctgcg gcgcggcgac
     6541 cacgtcggcg aaatgccgcc gcacgtgcgc gcgatggcgc tccagttcgg ccgtgaaggc
     6601 ggaccagtcg ggcaagccca gcgcgaaggc aagccgcagc ctgtcgtgct cgtcctcggg
     6661 cagggcctgg gtctgctgat cggcccgggc ctggatcgca tgctcggtgt tgcgcaggaa
     6721 cacgtaggcc tcggcgagct gcgccaccac cgcggcgggc aggtggccca ggctgccgag
     6781 cagttccagc accaccagca ggcggcgctc ctgcagcgcg gtctcgcggc cgccgcggat
     6841 cagctggaag gactgcgcga tgaactcgat ctcgcggatg ccgccggcac cgagcttcac
     6901 gtcggtggtc ttgccgcggc gctgcacctc gcggttgatc atcgccttca tgccgcgcag
     6961 ggattcgatg gcgctgaagt cgatgtaacg gcggtaggtg aacgggcgca gcgccgcgag
     7021 cagctgctcg ccggcggcgc gatcgcccgc gatcacgcgc gccttgatca tcgcgtagcg
     7081 ctcccagtcg cgaccctggt cctggtagta ctcctccatc gcgtcgaagt tcagcgcgag
     7141 cgcgccggcg tcgccgtagg gccgcaggcg catgtcgacg cggaacacga agccgtcctg
     7201 ggtcttggcg tcgatggcct tgatcacgcg ctgcccgagg cgcgtgaaga actcctggtt
     7261 gctgagcccg cgctcgccgt cggtctcgcc ctgctcgggg taggcgaaga tcaggtcgat
     7321 gtccgaggag aggttcagct cgcaggcgcc gagcttgccc atgccgagca ccaccaggcg
     7381 ctgcggcgcg ccggactcgc gcccgcgcgg ctcgccgtgg cgcgccgcga gctgggcgtg
     7441 cccgtgcgcc acggccgcct cgaccgtggc ctcggccagc caggtgagat cgcgcgtggt
     7501 ctcgacgagg tccgccgcgc gcagcaggtc gcgccacacg atgcgcacca tctcgcggcg
     7561 gcgcagccgg cgcagctccg tggcgagccg cgcctcgtcg gcatcggcgg gcagcgcgag
     7621 cgccaggcgc tggcggaatt cgcagtcgtc gtagctgcgc gcgagatcgc cgctctcgag
     7681 cagccccggc agcagctcgc tgtcgcgcgc gaacgcctgc gccacgaact ggctgcagcc
     7741 gagcacccgc gccagcgccg cggccagcgc ggcatcggcg gcgaggcgcg cctgcacggc
     7801 ctcgcgcgcg gcgggctcca gcgccgcgag gatttcctgc cagtcgcgct ccagcgccgc
     7861 atgcaagggc aagggcacgt gctccagcgg caatgtgttc atgcaccgtc cctgctgttg
     7921 tccatcggcg ccacgcgcag aacctcctcg agtgtcgtga gcccggcggc gatcttgcgc
     7981 gcgcccgcca ggcgcagcgt cttcatgccg gcgcgcatgc cgatcaggcg caactcctcg
     8041 ctgtcgcagc cctcgcgcac cgcgcgctgc accgcggtgt cgacgcgcag gatctcgtag
     8101 atgccctggc gacccgagaa tccggtattg cggcattcga gacagccgtt ggcggcgtag
     8161 accgttgccg gcggcttcgc cttccacggc gcgaccaggc gactccacac gtcctcgtcg
     8221 atcgcggccg gcaccttgca gtgcgggcac aggatccgca gcaggcgctg cgccatcacg
     8281 ccgagcacgg tggcgcggat caggtaggag ggaatgccga gctcgagcag ccgcgtaatc
     8341 gccgaggggc tgtcgttggt gtgcagcgtc gagagcacga ggtgcccggt cagcgcggcc
     8401 tgcaccgcca tctcggcggt ctcgcggtcg cggatctcgc cgatcatgat gatgtcggga
     8461 tcctggcgca gcagggcgcg cacgccgctg gcgaaatcga gaccgatgcc gtgctgcacc
     8521 tgcacctggt tgaacgcggg ctcgaccatc tcgatcgggt cctcgatggt gctgacgttc
     8581 acctcggccg tggcgagctg cttcagcgtc gagtacagcg tggtggtctt gcccgagccg
     8641 gtcggcccgg tcaccagcac gatgccgtgc ggctgctcga ccatgccgcg ccactgcgcg
     8701 tggtcctcgt cggccagccc gaggtcggcg aagctgcgca ccagcacctc ggggtcgaag
     8761 atgcgcatca ccagcttctc gccgaaggcg gtcggcagcg tcgagaggcg cagctcgatc
     8821 tcgctgcccg cgggggtgcg cgacttcacg cgcccgtcct gcggccggcg cttctcggcg
     8881 aggtccatgc gcccgaggat cttcagccgg ctggtcacgg ccatcgccac cggtgtgggc
     8941 agctggtaga cgccgtgcag gatgccgtcg atgcgaaagc gcacgttggc gagctcgcgg
     9001 cgcggctcga ggtggatgtc gctggcgcgc tggtcgaagg cgtactgcag cagccagtcg
     9061 acgatgctga cgatgtgctg gtcgttggcg tcggggtcct tgagcttgcc gagctccacc
     9121 agctgctcga agttcgccag ccccgcggtg gtctgcacgc cgcgcgcgcc gctggcgccg
     9181 ctgacggact tcgccaggct gtagaactcc acggcgtagc gctcgatgtc ggcggggtcg
     9241 gcgatcacgc ggcggatcgg gcgcttcagc gtgcgcgcga ggtcgggctc ccaggcgctc
     9301 accagcggtt cggcgccggc gatcacgacc tccgcggccg tgctctcgac ggcgaggatg
     9361 cggtggcgct tggcgaaggc gaaggacatc accgccgtga ccgcggcggc gtcgatgcgc
     9421 agcggatcga tgtggtagac ggcctgcccg gtgcgctgcg ccagccagcc gagcaggcgc
     9481 tcgacaccca gcggccgggc gtccgccggc gcgaccagct gctggtcggc gacgaaattc
     9541 accgggtgac gcgccacgcc ctcgcgggtg cgcggcgcgt agcgcagccg gcgcgcgtcc
     9601 gccgcgtcga tcaggccgtc ggcggccagg gcgtccagca gctcgccctg gtcgatcggc
     9661 ttgccggcgc tcagcgtctg gggttcggac atgcggtgcg gattcctgcg atggtgcaga
     9721 cggatggtag ccgatcctgg caatgctgcc gcggcgcgct tgggatgggt gtgcggcgcg
     9781 gaatcagcgc cggcgcttgc ggccccgccg cggcgcgctc cccggccccc ggaacggatc
     9841 gaagtccatg tccacgaacg catccggcgc atccaggaag gcatcgagca gcccgtccag
     9901 tcgctcctcc tcgcgcagat cgcgcgcgta ctgctgcagc cagcgcttgc gctgcgcggg
     9961 ctcctgcatc atggcgtggt gtgccttcag cgccttgacg gcgcgtttca gctcggcgag
    10021 ctcgcgcttc agggcgtgct gcaccgaggc gggagtcacg acggccgggg cctggccggt
    10081 caacccggcg aaatgccccg tgatgccggc cagctcctgc tccagttccc tgagctgctc
    10141 gcgcagcacc ttgttgtagt ggcccagacg cgccgtggac acgcgcgcca ggtgcgcggc
    10201 gtcgatctgc tcggtctcca gttgcagggt gagcagcgtc agcaggtcgc cctcgtcgta
    10261 ggcatggttg acgcgctgca tcagctcgtg gcgacgcgcg cgctcgacct cgtcgccggc
    10321 gcgatccgga tgcagggcgc tggcgagctt gcgaaagacc tcgcgcacgg actggctggc
    10381 ttccttcgcg gcctgttcgg cggccgcggc cttgtccgtg gcacgcgtgc gcgctttcgc
    10441 ccctgcggcg cgccgggcac gcgcggcttg cgcctcctcc tcggcggcct gccgggcctt
    10501 ggccgcagcg gcggccatcg cctcctcgac cgagctgccg agctcgtgct cttccagctc
    10561 gacgccgaac atctgctccg ccatcccgcg cgccagctcg cggtcgagct cctggtcctc
    10621 ggcaaagccg atatcggcgt actgatcgtg cagggcaatg atctgctcat cctgcggatc
    10681 gtcctccagc tgggcgcgcg cgagctccag caggtggtgc acgaggttgc gctgctccac
    10741 cttgcccggc ctgccgccgg cggccttgcc ctcgaggatg cgcgccagcg tcaggatcgt
    10801 ctcgcgccgc gccgcgccca actgcttcag caccggcatc aggtccttgg ccacgcaccg
    10861 gtggtgctgt ggctcgaaag cggcccactc ggcgagcttc tcgcgcagta tctcgacctt
    10921 gcgcaccagg cggttgaagg tctgctgctc cttgcccagc ggcgccgcgc cctgcggcgc
    10981 gatgcgcagc tcctgcgcgg gtggcggccg cggcgacgca tcgaagagat ccgtttgcga
    11041 atggtgcggt ttgcttgctg tcacgggctg acccagcgtc aagggttgtg gccgcgtcgg
    11101 gccgttcggc cgccgactct agccgcaccg cgcgaaagac tccagactac gcgggcggta
    11161 cggatggggt cggagcgaca ggatgacgga gcagatcgac acggcggtcg aggatttcat
    11221 cgtacggtgg gagggcgcgg gcggcagtga gcgcgccaat taccagctgt ttatgaacga
    11281 gctgaccgtg ctgctggctg tggagcagcc gcacccccag gcgcaggacg cccgcgacca
    11341 cgcctacgtc tacgaacgcc gcgtcacctt ccgccacggc gacggcagcg agagcaacgg
    11401 ctatatcgac tgctacaagc gcgcctgctt cgtcggcgag gcgaagcggc tgaaggccgc
    11461 gccggacacc cggggattcg acgacgcgat gctgcgcgcg cgcagccagg ccgagcagta
    11521 cgcccgagcc ctgcccgccg acgaaggccg cccgccgttc ctggtggtct tcgacgtggg
    11581 ccggcgcatc gacctgtact cggacttcac gcgcagcggc gcgacctacg tcccgttccc
    11641 ggatccgcgc agccatcgca tcgcgctggc ggacctgcgc cgcccggata tccgcgaccg
    11701 gctgcgggca gtctggaccg aaccgctctc gctggacccg gcgcaggccg cggcccgcgt
    11761 cacgcgcgag atcgcggcgc ggctggcgga gctcgcaaag tcactggagc gcgccggtca
    11821 cccggcgcaa ccggtcgcgc agttcctgat gcgctgcctg ttcacgatgt tcgccgagga
    11881 cgtgcgcctg ctgccgccca acagcttccg cgacctgctg gatcgctacc gcgagcagcc
    11941 ggataccgcg atgcgcatgc tcgaacagct gtggcgcgac atggaccgcg gcggcttctc
    12001 gccggtgctc gccgtcgatg tgctgcgctt cagcggcaag ctgttcaggg cgcccgatac
    12061 actgcccctg gaccgcgacc agatcggcct gctgcacgcg gcggcgcgcg ccgactggcg
    12121 gctggtggaa ccggcgatct tcggtaccct gctggaacgc gcgctcgatc ccgcggagcg
    12181 ccacgcgctg ggcgcccatt tcacgccgcg cgcctacgtc gaacgcctgg tgctgcccac
    12241 cgtgatcgaa ccgctgcgcg cggagtggat cgaggcccag gccgcggccc tgacggtgtc
    12301 gggagaaagc cgtgacgccg aggccgtggc gatactgcgc gcctttcatc atcgcctgtg
    12361 cacggtgcgc gtgctcgacc cggcctgcgg ctcggggaat ttcctgtacg tgaccctgga
    12421 gcacctgaaa cgcctggaag gcgaggtgct gaacgcgctg gacgagctgg gtgcgagcca
    12481 ggtgagcctg gcactggacc acgagcgcgc cgatgcgagt gccggcgaga ccgtggaccc
    12541 gcaccagctg ctcggcatcg aactcaaccc gcgcgccgcc gccatcgccg aggtggtgct
    12601 gtggatcggc tacctgcagt ggcactttcg cacccacggc caggtcaatc cgccgcagcc
    12661 ggtgatccgc gacttccgca atatcgagaa ccgcgacgcc gtgctcgcct acgaccgcgt
    12721 ggagtacctg agcgacgaag gcggcgtgcc ggtcacccgc tgggacggca ccacgtacaa
    12781 gacctccccg gtcaccggcg aacagatccc ggacgagagc gcgcggattc cggtcgagca
    12841 ctacgtgaac ccgcgcaagg ccgagtggcc cgaggcggat ttcgtggtgg ggaatccgcc
    12901 ttttataggc aacaagcgga tgcggatcgc gctcggggat gggtacgtcg aggcgctgcg
    12961 tgaagtgtgg gcggcggtgc ctgattccgc cgactttgtc atgtactggt gggagagcgc
    13021 cgcccaattg gctcgcgagg gcagcatttc acgcttcgga ctcattacga cgaacagctt
    13081 gaggcaggcg ttcaacaggc gcgtcctgga gctgcatctc ggagcaaagg aaaacccgtt
    13141 atcgctaaca tttgcgatcc cggatcaccc ttgggttgat agcgtcgaag gagcggcagt
    13201 aagaatcgca atgactgtag gtagacaagc ggcggcatct ggggtcaatc aaactgatgg
    13261 tgttcttctt aactgtgaac aggaagtatc gggcgacgcc ggagagtaca ttgtgcgttt
    13321 ccgccgaagt gacggaataa tcaacgccga tttatccata ggttccgatc tcaccagagc
    13381 gtcccgactt gctgcaaacc gaggtctcgc ttgccccggg gttcaattat cagggcaagg
    13441 cttcgtgctg ttgccggaag atttgcatcg attttctatc actgcgcgac agcgcgtcat
    13501 tcgtccttac attacaggac gcgatatcac ccagcggccg cgggcgcagt tcgtgattga
    13561 tacatttggt cttgcagagc tcgaactcag atctgcttac ccagatcttt accaacacct
    13621 cgcaaacacc gttctccctg acaggctagg ggtatcgacg ggaagcaagg atgtcgccaa
    13681 atacgcgaag gagtggtggc ttcattcaaa accgcgaccg aaatttaggc ccgcgctttc
    13741 tgggctcgat cagttcatcg taacctcacg cacggcgaag caccgaattt tcgtgttcgc
    13801 cgaagcagac acgcttcctg aaaccaaggt tctgatcgtt gcgacaggcg acgcagcgta
    13861 cctctcaatg ctttcctctg cggttcacct attgtttgca gattgcgctg gtggccgtca
    13921 tggcgttggc aatgacctta cctacaacca ttccgattgc ttcgagaaat tccccttccc
    13981 catcctgagc gacacccaga ccaccaccct gcgcaccctc ggcgaacaac tcgacgcgca
    14041 ccgcaagcgt cagcaggccg cgcaccccgc gctcacgctg accggcatgt acaacgtgct
    14101 ggagaagctg cgcagcggcg acacgctgag cgacaaggaa cgcgcgatcc acgagcaggg
    14161 gctcgtgtcg gtgctgcgcc agctccacga cgagatcgac gccgccgtgc tcgatgccta
    14221 cggctggagc gatctgctgc cgctgctgcg gatcgcgcac ggcaacacgc cacccgcggc
    14281 cgaccagacg cgcgaggatg ccgggcgcgc cttcgacgcg gccgtgctcg agcggctggt
    14341 cgcgctgaac accgagcgcg ccgcggaaga agcgcgcggc ctggtgcgct ggctgcgtcc
    14401 ggagttccag aatccgcagg cgagcgcagc gccggagcag gaggcgatgg acgtggccgc
    14461 ggcggacgag gatatcgccg cgccggttca cgcgacgaag cccgtgccgt ggccgaagga
    14521 tacgttgcag caggtgcgcg ccgtggcgga ggcgatcgcg gccagcgccg cgggcctgac
    14581 actcgcggaa atcgaagcac gcttcaccgc gcgcggtccg tggaaacgcc gcctgccgat
    14641 cgtgctcgac acgctcgccg cggtgggtcg cgtgcgcgaa cacgagggcc ggtatcgcgc
    14701 gatgtaggtc cggctgaagc cggacccacg gtaggacaaa tcggtataag cgtttgctgg
    14761 agccgcgcaa cgatgctgta taaaataaca gttatcgctg ccggaggccg attgccatgg
    14821 aaacctgcac gccttcccct tgttcacgcg gtgccggact cgatcccgaa tcgctctccg
    14881 ccgccgcgct cgaagacgag atccacacgc tgagcgcgag catcaccgtc gcgacctggc
    14941 gattgttgat gctcatcgcc gaactcgacc gtcgccgcgc gtggcagcat tggggcgtta
    15001 tctcctgcgc gcactggctc aactggaagt gcggcatcga cctgggcgcg gcgcgcgaga
    15061 aggtgcgcgt ggcgcgggca ctggagaatc tgccgctggt caacgatgcg ctcgcccggg
    15121 gcgagctgag ctactcgaag gcgcgcgcga tcacgcgcgt cgccacgccc gagagccagg
    15181 actacctgct gatgatcgcg cgccacggca cggcctcgca cgtcgagaag ctggtgcgcg
    15241 cgtatcgcca cgtcgagcgc ctgcaggtgt cggcgcgcgc acgcagcacg cacgcgcagc
    15301 gtgcattgcg ctggagattc gaggatgacg gttcgatggt tatcacagtg cgccttcccg
    15361 tcgaacaggg cgcattggtg gtgcaggcga tccacgccgc ggtcgatgcg caatggcgcg
    15421 agcgcgacgg cgccgatcgc gagcaggaac tcgcggacga cgtttccgcg gaaacgtcac
    15481 cggcatcgag cccgtcatcg aacagcttcg acaatgccga atcgcccgtg tccgcgcgcc
    15541 gcgccgatgc gctgtgccat gtggccgagc agttcctcgc ccacgaggcg gtcacttcac
    15601 gccaggccga gcgctaccag gtcgtggtac acgtggaccg cgcggtgctg tcggagcacg
    15661 gtcacgggga ctgctgtcat atcgagcacg gcagcgcgat tgccgccgaa acggcccgtc
    15721 gcctcgtatg cgacgcctcg gtggtcacat tggaagaaag cggagacggc gagccgctga
    15781 acatcggccg ccgttcgcgc acgattccgc cggcgatacg ccgcgcgctg gcgcaccgcg
    15841 acaagggctg ccgctttccg ggctgcctga atcatctgta cgtcgatgcc caccacatcc
    15901 ggcactgggc cgacggcggc gagacgcgcc tggacaacct ggtgcttgcc tgccgtcacc
    15961 accacggcct gctgcacgaa ggcggcttca cggtggcgcg gcgcgacgac ggcaagctgc
    16021 tgttcttccg gcccgatggc gagttgctgc ccgaggtgcc gaagccgccg ctgcgcgtgc
    16081 acgacatcga agccatcgtc gcggaatcgg gcatcgacgt ttccgcggag acgtcgcgct
    16141 cgctgtggga cggcagccgg atggacctgg atatggcggt cggggggttg atgacgctga
    16201 acgggtggcg atgaggagcg gacgcgcccc gaccgcagct ttcacccgca ggagatgtcc
    16261 cggctgaccg gtcttcgaag aacctggtgc gcgcgtatcg ccacgtggtc tctacttatc
    16321 agtggtttca tcgcgtgcta gattcaagca caggacactg tgcttaactg gcaatcttca
    16381 tgaaagacgt ttcaactcaa caggttgcag agcatcttaa gcggctgaac ccgtggtgga
    16441 ccagcggccg gatggacgag gacacgctgg tgctaagacc gcgcgcctaa gggcacgcgc
    16501 tacctgcgca ttctcgtatc cggctcggcg gcggcagcgc tcaagcggca aagcaccgaa
    16561 tccggcgcgg ggcgtttcac cgacttcctg ctgccgcccc tcaccttctc cgagtacctg
    16621 caactccggc cagagcccgc ggccatccgc gaggaagagc ccgggttgta tgcactggaa
    16681 gacatcggtc gactgaacga acagttcgtg gagtacgtga atttcggggg ctacccggaa
    16741 ttggcgctgt cacccaccgt gcgcggcaac cccgaacgct ttgtcaaatc cgacattgtc
    16801 gacaaggtgc tgctgcgcga cttgccgcag ttgtacggca tcaaggacat ccaggaactc
    16861 aattcgctgt tcaccctgct tgccttcaac accgcagagg aggtctcgtt cgagcagttg
    16921 tcgcagcgca gcggcgtcgg caagcagacg atccagcgat acatcgagta cctggaagcg
    16981 gcgtttctca tcaaacgggt gttccgcatc gaccaggacg gcaagcgcta ccagcgcgaa
    17041 cggaatttca aggtctacct gacgaattcg gccatgtaca ccggcctgtt cggcgccaag
    17101 gatccggacg agcgggagtt cgggcacctg gtggagaccg ctctgttcga gcagaggttc
    17161 cacgaagacg cgcggctgaa ctacgcacgc tggggagccg aggacagtga ggtagatctg
    17221 gtggagtcgt ctgcggcact gaagccgatc gcggcgctgg cattccacta cggggcgcgc
    17281 gccgtgcagg gccggctggc gggctacgag gcgcagatcc agggcgtgac cgcctgaacg
    17341 atcggcgtct gttcgtctgg atcgcgaatt gtcggaaggg ctcgtactgc cggagcacag
    17401 cctcgctcgc tccgggatgg cagccggatg aatccggacc cacgctaact tatactcggt
    17461 cgcaaccggc cgtttcagga gccgggatca cgaaccggac ggatcatgtc gcgccccgta
    17521 cccgactttc tcacgatgtt cgaccgcctg ctggcctcgc cgtcggtgag ctgcaccgag
    17581 cccgagtggg acgagggcaa ccgcggcgtc tgcgagctgc tggccgaatg gctgcaggcg
    17641 ctgggttttc gctgcgagat acagcagatg ccctcgcgcg ccgacaaggt caacctgatc
    17701 gcggtgctgg gcgagggccc cggcgggctg gtgctcgcgg gccacaccga taccgtgccc
    17761 ttcgacgaga agctgtggga gatgaacccc ttcggcctga cggtacgcga cgagcgcctc
    17821 tacggcctcg gcgccacgga catgaaaggc ttcttcccga tcgccatcga ggcggcgctc
    17881 gccttccgcg gggcgcgcct gcgcgagccg ctgatcctgg tagcgaccgc cgacgaggag
    17941 agctcgatgg acggcgccaa catgctggtg gccactggca agccgcgtgc gcgccacgcc
    18001 atcatcggcg agcccacgga cctgcgcccg gtgcgcatgc acaagggcat gatgatggag
    18061 gcggtcacgg tgaccggaca gtcggggcac tcctcgaacc cggcgctggg acgcaacgcg
    18121 ctcgagggca tgcagcacgt catggccgat ttgctcgcgt tccgcgacga gctggccgcg
    18181 cgccaccgca acccgctgtt cgaggtcgag gtgccgacgc tgaacttcgg ctgcatccac
    18241 ggcggcgaca accccaaccg catctgcggc gagtgcgagc tgcacttcga cctgcgcccg
    18301 ctgccgggca tggacctcga ggacctgcgc gccgttatcc gcgcgcggct cgatggcgtg
    18361 gccgcgcgca cggggctcgg catcacgatg cgcccgctgt ttccgggcct gcatcccttc
    18421 gccgaggatg cggcggcgcc gatcgtgcgc gccgccgagg aattcaccgg ctacagcgcc
    18481 ggtaacgtgg ccttcgccac cgagagcccg ttcctgcagc gcctcggcat gcagaccgtg
    18541 gtgatggggc cgggctcgat ccgccaggcg caccagccca acgagtacat ggagcagttc
    18601 caggtcgaac cgatggtgac ggtgctgcag aagatgatcc agcgcttctg cgtggacggg
    18661 cattcgtgat gatgagcgga tctttccccc agttcgtcaa ttccttccgc aactccgcgc
    18721 cgtacatcaa cgcgcacaag ggcaagacct tcgtgctggt gtgcggcggc gaagtggtgg
    18781 ccgacgagcg cttctcctcg atcgtgcacg acatcgcgct gctctcgacg ctgggcgtga
    18841 acctggtgct ggtgcacggc tcgcgcagcc aggtcgaggc cgggctgcag tcggcaaacg
    18901 cggcgagcag cgtggtgcac ggcacgcgcg tgaccgacgc gcgtgcgctg gagctcgcgg
    18961 tgcaggccgc cggcatggtg cgcgcgcgca tcgaggcgct gctgtcgatg ggcatcgcga
    19021 actcgccgat gcacaacgcg ggcatccgcg tctgcggcgg caacttcgtg accgcgcagc
    19081 cgctcggcgt gctcgggggc gtggactacc agcacaccgg caaggtgcgc cgcgtcgacc
    19141 acgaaggcat ccgcgcgcaa ctggcgctcg ggagcatcgt gctgctgccg ccgctcggct
    19201 actcgccgac cggcgagatc ttcaacctca ccaaggagga cgtggcggtc tcggcggcca
    19261 cggcgctgcg cgccgacaag ctgatcctgc tcgagggcgc gcccctcacc gacgcctcgc
    19321 gccagccgct gcgcgacctg ccgctcacgc tggcgcagac ggtgctcgcc ggcaacgcca
    19381 cgccggcgct ggccgccgcg atccaggcct gccagcaggg cgtggagcgc tgccacatcc
    19441 tcgacttcca caccgacggc gcgctgctgc acgagctgtt cacgcgcgac ggcatcgggg
    19501 cgatgatcaa ccgcgatgcc tacgagagca tccgcatcgc cggcatcgag gacgtgggcg
    19561 ggatcatgga gctgatcgag ccgctggagc gcgacggcac gctggtgcgc cgctcgcgcg
    19621 agcgcctcga gctcgagatc gcctgcttca gcgtggtcga gcgcgacggc gcgatcatcg
    19681 gctgcgcggc gctctacccc gacctgccga accgcaccgg ggagctggcc tgcgtggcca
    19741 ccgacccggt ctaccgccgc agcggccgcg ccaaccggct gctcgaacac atcgaggggc
    19801 gcgcgcgcgc cgagggcatc gaccgcctgt tcgtgctgac cacgcgcacc gcgcactggt
    19861 tcgtcgagcg cggcttccgt cccgcggagc tcgccgaact gcccgagaag cgccgcgagc
    19921 tctacaacta ccagcgcaac tcgctggtgc tcatcaagga tctgtcctga tcctcccgcc
    19981 agtgccacgc ggactgtccc ggggtcagtc cggtaagcgg cgcagttctg gcgcctattc
    20041 ggcaaacttt cccgatcggg aaatatatag ccgatccttg acctgaccgc ccgcaaaatt
    20101 cccgatcggg aaatattgct cgcactgaca ccagttgtgg ctgataattt cccgaacggg
    20161 aaatcgtaaa ggccatgaca accattcgat cacctcgaca actcggcgat gcacttcgtg
    20221 ccgctcgcaa gcagctcggg ctgacacagc cccagttggc tctggcggca ggggttggcg
    20281 tgcgtttcat cgtcgacctc gaggcaggca aacccacctt gcgactgcaa aacgtgctgc
    20341 gggtcattga tgcgctgggt ggtgagatcc agttgcgcga cttgccatct gtcgtggccg
    20401 acgatcaacg ggaaggtggt gaccatggcg catgagctgg acgtctggct tttcgccgat
    20461 cgtgtcggca cgctggcgct ggtcgatggc cgactggatt tttgctatgc gtccggctgg
    20521 ctgtcgcgtc cagacgccgt tgccttgtcc gcctcgctac ccttgcaagc ggagccgttc
    20581 gacgatcgca aggcgcgccc cttctttgcc ggtctgttgc cggaggggca gatgcgccgc
    20641 ctgattgcgc agcagttcca ggtttctggc cagaacgatt ttgcgctgct ggagcacatc
    20701 ggcggcgaat gcgccggggc cgtgacgttt ctcgaaccgg ggcaggcttt gcctgtgtca
    20761 acccgcaacg atgacgtcca gtggctgagt gacgaggaag tcgttgccat cctggatgaa
    20821 ttgccacgcc gccccatgct ggcgggcaaa gacggcttgc gactttcttt ggctggcgcc
    20881 caagacaaac taccggtggt gttcgatggt gcccgcatcg gcctgcccct caacggcacg
    20941 cccagctccc acatcctgaa gcccgccatt caggctgttg aagacagcgt gatcaacgaa
    21001 gggttctgca tggcactggc tgatgccatg cagctcaaac cggcaaagtc aaaggttcat
    21061 cgcgtgttgg ctcgctcgtt cctgctggtc gagcgctacg accggcctat cgatgctcag
    21121 gggtactggc aacgcctgca tcaagaggat ttctgccagg cacttggcgt ggccccggag
    21181 atgaaatacc agaacgaagg tggacccgat cttgcgcgat gctttgatct ggtgcgccgg
    21241 gcaacgcgcc cgaatgcacc gcagattctg cgcctgctcg actacgtgat cttcaatgca
    21301 ctgatcggca atcatgatgc acacgccaag aatttctcgc tgctgtactc tggcaagagc
    21361 cccgttttgg caccgttcta cgacacgcta tcgacggcgg catatccaac actgacgccg
    21421 aagatggcca tgaagatcgg cagcaaatac aagttcagcg aagtccgggc gcggcactgg
    21481 gagcagtttg cagaaagcgt cggtctcacg aaggcgcaag ccaggaggcg catcctggaa
    21541 ctggcaaagt cactgccaac caccgcccgc aagctgcagt ctgaccccgg acgcggtttc
    21601 gccggcaatg ccctggtcga gcgaatcaat gccttgatcg aacaacgctg cgccctcacg
    21661 attcggcggc tgaccgatcc cgccgccgaa aaagaagcgg acgccaatcc gtctgcctga
    21721 atcaccatac gttccctgca accagcctgg cagaagaacc tgcttgcagg tctacaacta
    21781 ccagcgcaac tcgctggtgc tcatcaagga cctggcgtag gtccgcatac acgcggacat
    21841 tgtccggctg aaaccggacc t
//
LOCUS       DAORUQ010000029        21816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1986_length_21816_cov_6.151877, whole genome shotgun sequence.
ACCESSION   DAORUQ010000029 DAORUQ010000000
VERSION     DAORUQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21816
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1986_length_21816_cov_6.151877"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLW13_04420"
     CDS             <1..569
                     /locus_tag="PLW13_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69645.1"
                     /translation="GYEMKIFDDDGNECPPGVPGEICCRPAAGGTASVDVEYLGNPEA
                     SKQKIRAGWNRSGDVGHSDAEGWLYFDYRKGGGIRHNGDFINTGFVEKIIAESALVDD
                     VFVYGVPALSGAPGEKDVVAAVVARDPAAFDPAALFAHCRAGLEPNFVPSYLQVVDEI
                     PKTASEKPQERFLLEAFSRDGTGIHALR"
     gene            582..1571
                     /locus_tag="PLW13_04425"
     CDS             582..1571
                     /locus_tag="PLW13_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY69646.1"
                     /translation="MSFVHVTGHASVLWTPVSHATIAAACHNGFGEHARLVACEAVTH
                     GGFNTIYRVHMAAHEPVVLRVSPPRRAPLLRHERELLAREAAVHARLASIGPVIPRIL
                     HQDFSCTTLPRPYLFLEHRRGQPWDDVAPRIAPAQAASLWSEFGNHVRKIHAIEGEAF
                     GSPLRGEGRASHAEWLLSLFDDLATDLAERKLLVPGLTEFRIALQQRREQLELHTPPR
                     LVHGDLWPRNVLVTRERGGWRLSAILDAERAFWSEPAAEWIFSHLEIPPAFWEAYGAD
                     LSPGRLDGDAALRTLVYRARGALQLVLQCYRSGASAAFALARFRACAREFARH"
     gene            complement(1641..3038)
                     /locus_tag="PLW13_04430"
     CDS             complement(1641..3038)
                     /locus_tag="PLW13_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQY69647.1"
                     /translation="MSRMWITLCAALLGGCQSADFVRPDVPVPDTWPHLGADSSPATA
                     AASLADRLWAEVLPLPELTALIDEALAGSSDLRIAVERIELARAQYGLERSAMFPGID
                     LSGSVTRQRAPGFDPAKNTIGESSGLALAVPAWEIDLWGRLADRTEVARREVLASEAA
                     AQGVRISLVADVSARYLDLLDLDNQLEISHRTLESRRQSLRLTRARFDEGISSIVDVR
                     QAESLLASSEVALADQRRRLAQAENSLAVLLGRNPGSIERHVRLDAIELPATMLAGIP
                     SALLPRRPDIQAAEERLRGAHASVSAARKAYLPSLSLTTTLGLASPDVGGLFDSGRHA
                     WSVQPAAALPVFTAGALDSGVALAESQQRILVEQYKATIRQAFREVSDALVAIEQLGA
                     QRAASRRVVLANRERLRVTKSRYVGGITSYFEVLDAERQLFDSELSLSQSTRALHQAL
                     VQLYVALGGGWSGAA"
     gene            complement(3035..6091)
                     /locus_tag="PLW13_04435"
     CDS             complement(3035..6091)
                     /locus_tag="PLW13_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY69648.1"
                     /translation="MNVAGYTLHKEVVAWVLAALVLVGGYLAYERLGRFEDPDFVIRQ
                     AVVLTAYPGASPAQVAEEITDLVEGAVQQMQEVEEVISVSRAGESLVKVEAKLEFSRT
                     QAELDQVWDKLRGKIADVSRALPPGAAQPVVNGDFGDVYALFYAVTGEGYTPRQLYDY
                     LDLLRRELVLVPGVARVALLGERTEAIFVEISRARAAQFGIPLDQVYATLRAQNVISP
                     AGNVRAESLRLQINPEGTVESIDTLKDLVVAGDATSGLVRLQDIAEIHRDYITPPTVL
                     VRRDGRPAIALGISNVLGGNVVAMGDAVKQRLVELESQRPVGMELNPVSLQSDSVRAA
                     VDGFVENLVAAVAIVVVVLLIFMGLRSGLIIGAVLVLTVAGTMVAMLMSDIAMQRISL
                     GALIIALGMLVDNAIVVTDGILVRMQRGEEAVPAAVAVVQGTIWPLLGGTLVGILAFS
                     AIGLSPTDMGEYAGSLFWVILYSMLLSWVFAITLTPLFCVAFLRVKAVAGDARGGGLM
                     AAYSRLLGSVLRHRRASTMALLGLFTAGVIAFGFVPPGFMPDSARPQFVVDLYLPQGT
                     DIETTAEMVAAAEALVRAKPGVTHVTSFVGQGALRFMLTYSPEDPNPAYGQLLVDVER
                     AGDIAARIPELQRELEQAFPSADIKAWKFMLGRGGGKKIEAAFRGPDPLVLRGLAEQA
                     RAIMANDPEALAIQDDWRQQVPVVRPRFSVEAAQRAGVLPSDIAAALERNYTGQQIGV
                     YREREKLIPILARSPAAEREGVEDLGNVQVYSKLAGRFVPVDQFIERVDTRWEDAIIR
                     RENRFPVIKAQCDPPAGSLSAPLFERLRPQIEAIPLPPGYTLEWHGEHKASTEANAGL
                     VSAAPYGMVAMVLAVIVMFNAIRQTAVIWLTVPLAVVGVAIGLLLFQAPFEFMAILGF
                     LSLIGMLIKNAIVLVDQIDVEIRSRKPRFQAIIDAATSRARPVFLGAVTTVLGVAPLL
                     LDPFFRSMAVTIMFGLVFATGLTLVVVPLLYAAFFGIRADEVESVR"
     gene            complement(6088..7209)
                     /locus_tag="PLW13_04440"
     CDS             complement(6088..7209)
                     /locus_tag="PLW13_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY69649.1"
                     /translation="MAELLHATGGPAKACAPGRVRAALVFAAIFCLAGCGESQAPLEA
                     TAAARPALIHEVRSSAAGGKLRFPGRIRAERRAELSFDVPGFVAEFVPIAGSHVQAGQ
                     VVARLDDAVFRARVTAAGAEFDRAKADLERYQRLWETERAVAPSEVDERRSRLEVART
                     NLATANRELADTVIRAPFAGVLTRRRLETFSNVQAKQPIADLQDLDALEVVINVPARV
                     VRTATEQQAAAAIFEGEDTRSIPLKLKSFATDADPQTQTYEIVLSLGALPPDMNVFPG
                     MSVTVLPFVGAAAEPGATVSIPLTAVATDASGATFVWVLDAQSRAERREVRTGAIRGG
                     DITIATGLTQGERIIAAGVSAVRAGMQVRPLSATGADPA"
     gene            complement(7277..7642)
                     /locus_tag="PLW13_04445"
     CDS             complement(7277..7642)
                     /locus_tag="PLW13_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69650.1"
                     /translation="MLLAAVLPAEARSDRWQVRKEMREGTRNVEREKREAQRALRRCE
                     TRECARREMREGYREVEREKREARREIRGAMRDSRWEDERWRDRRDRVDDHAFLKGAL
                     VGAALVGVTAAIIEANDDD"
     gene            complement(7684..8082)
                     /locus_tag="PLW13_04450"
     CDS             complement(7684..8082)
                     /locus_tag="PLW13_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69651.1"
                     /translation="MNIRHIFVTLAAVSFCLGLQGLQAADPASPVTASAAPDPEEQLD
                     EGLQRFGYLAGLARGCVVEAQRPALESEAVDLHGAIGRLLGTDRAFMFGAAFGHGTSL
                     TLEVAECKEVLAQYERRVQAFRASSGRVAP"
     gene            complement(8233..8661)
                     /locus_tag="PLW13_04455"
     CDS             complement(8233..8661)
                     /locus_tag="PLW13_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQY69652.1"
                     /translation="MITIIAVGLGVATACLGLQSLTALVALKYFIGGAAASGARRRAP
                     ITFRSMALLMLVLSIGSIVQMIAWALVYLALGQFGDFETALYFSGVTFTSLGFGDITL
                     IGTARLLAPLQAANGLMMFGFSTAVLINALHDSPARHGPR"
     gene            complement(8673..9149)
                     /locus_tag="PLW13_04460"
     CDS             complement(8673..9149)
                     /locus_tag="PLW13_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69653.1"
                     /translation="MADSEESFRRAFGLLAFAMNRHLAQHMRRVSRALDADLESVFVW
                     ATLAHMNAMNIALPGMDPLEAPTVGDAMLLALHPVRLTDVVQVTGLPRETVRRKLERL
                     REQGRVERLPDGSWLFRTDAIGSDAYEFTRDMVKNLLQTAAEVQRLLSEAGRERPV"
     gene            9414..10814
                     /locus_tag="PLW13_04465"
     CDS             9414..10814
                     /locus_tag="PLW13_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQY69654.1"
                     /translation="MSHENPGNLMSRREMLALGASALTLAAAPAFGLAAADAAPGPPS
                     GGDRVILSNEDSCTLTVIDPSTQRVKHTINLTSFDEDPRPPFRFVTGDMVPSHGAMIQ
                     KPLYHGAISVHGCAPSPDSRLLATVGRGSGNLYLIDTQSLAVVGNRPNPRAGPTTNAE
                     TLTSGVLVGREPHEPTFTPTGREIWVTLRGEDRIAVLDVDAIPAESSGQTARGASIRG
                     FVRTPPGPAMAWFADGGRTAFVVSQKTSSIEVMDVGYDASGYSTVGMRKTLDISAQDP
                     FGFSPFLRRSPDGREIWAVHKLADRVSVLSTGGVVEARVVETLAIGDKARPNHLEFVE
                     NANGRAVYVSCARLDTAGNGIASPIMIIDRSAPYGKRRIVGQFFSQGREAHGIWCDPS
                     ASMLFIAHELDELPQQPNAGQTVCTVFDVKDPFAPRFMTQIPLGDLALPSGRLRNKKS
                     VNLVYVRPGMPSATAA"
     gene            10825..11142
                     /locus_tag="PLW13_04470"
     CDS             10825..11142
                     /locus_tag="PLW13_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQY69655.1"
                     /translation="MNDYATYTREMHASMQQMMKDMHRDPPSGDPDIDFLAMMIPHHQ
                     GAVDMARLVIAAGRDPQVRVLAEAIMASQLSEIEGMRGRLAALRRGGSPDDGFPSPNG
                     TRG"
     gene            complement(11171..11806)
                     /locus_tag="PLW13_04475"
     CDS             complement(11171..11806)
                     /locus_tag="PLW13_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69656.1"
                     /translation="MGSGDDDRFVVITDGDALMNLTLFWRDAIPADWNGLPGLSRRIA
                     ASQPATMGDSNLAAVQSEQSVGVLGHGALVVNNEPPHIPWYLPKSFSPVLVGLLATHD
                     EHRPLGVQKFEWNAATRTLDEAWVNREVSSPNSVPMISAGSGMVYTEGARDGHWTLEA
                     LDWRTGTSRFHWVLPRARYNTMFAPVVLDPDGRLMWGGTWGRLRLDPRAGS"
     gene            complement(11894..12781)
                     /locus_tag="PLW13_04480"
     CDS             complement(11894..12781)
                     /locus_tag="PLW13_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69657.1"
                     /translation="MPESFSLHLRQRTVVTLCALLPLGVMADSATVEPRNPWLADSTY
                     PITHAASGQPGSVAVAGPVSSNRTLTDADIAYRFMGPAHLIPVISGPYADGRRVIWSV
                     GADRLVKQDYASFDIVDTLMLRENSPYTPAWAEQRERAIETSSGPLAVWYGVREMMLL
                     RESQGVYPLVDRDNEFYVLRNGGVEVYGDAQRGNAGSRIERKRRFEMPPQARGILVGL
                     NFTYDGWLVGVTDKGDVVAVARDFSRHYLAHLDGAPANDGDSSDTLSGWVRNSFAIGS
                     DNKVYIVSRDFMHCIRWTG"
     gene            complement(12810..14498)
                     /locus_tag="PLW13_04485"
     CDS             complement(12810..14498)
                     /locus_tag="PLW13_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69658.1"
                     /translation="MLDTIVRGGLVVDGSGASARIADIGIRNGVIEVIGAIAQAGAQE
                     IDARGLVVTPGFVDIHTHYDGQAVWDPYLSPSCLHGVTTVVMGNCGVGFAPVRAGERD
                     WLIRLMEGVEDIPGTVLAEGIDWQWESFPQYLAALRRKRFALDVAAQIPHGPLRTWVM
                     GSRGADPTMHPTEAEIRAMGELVSEALALGAIGFSTSRTVVHRSSDGKPTPSLSAKPA
                     ELLGIARAMRTSGRGIFEIVSDFSPLEHEFALIREIAAQSGRQVSVALTQTPEQPDAW
                     RRILELIAGARAEGLDIHAQVAARPISVLMGLSNRVNFLAVSASYRALMGLPLDEQAA
                     RLREPDTKRRILAELDLSLLPSAMLCDWNNIFRFDATLPYLPPRESSVAVQAARSATA
                     PEFLAYDWLLEGEGRHFLYTPVRNYAEASPAVLREMIQSELTVIGLADGGAHCAVLSD
                     AGMPTFVLTQWREQPDGKGFALEWLVQQLTQRPAEAWGFIDRGLIAPGKRADLNLIDL
                     ERLALRMPAWEYDLPAGGGRLVQDADGYVMTLVAGVPTVCDGVTTGLLPGRLLT"
     gene            complement(14524..15789)
                     /locus_tag="PLW13_04490"
     CDS             complement(14524..15789)
                     /locus_tag="PLW13_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69659.1"
                     /translation="MKQTLISADSHITEPPDLYVSRVDTKYRDRVPHLVRNGERGDMF
                     VMGDPLPPIPVGLIAAAGKDPIEIRTEGVKWEDMHRGGWDPVARLLEQDQDGVSAELI
                     YPTVGMMFCNHPDFELKRACFRAYNAWLAEYCASAPNRLLGIGQIAMHDPLEAIADLE
                     DMRRLGMRGVMLPGEPAQEDYDSPIYDDFFRAAVEMRLPVSFHILTSRGKQYRGSALN
                     GFMNTMRACQDVMGMLVFGGVFDRHPDLRVVSVEADAGWVPHYMYRMDHGYLRHRHHI
                     TPGKELQRKPSEYVSENIYVTFQDDWTAFGAAKAGMINPERVMWANDYPHSDSTWPWS
                     QPLLASQCDGMPREMLEKILFRNVAALYDIDLATIEQGKAELVPVAAGDEGVHGYNAS
                     SVKNAGRLADLPGMAAPVNFILAEEAPAR"
     gene            complement(15821..16027)
                     /locus_tag="PLW13_04495"
     CDS             complement(15821..16027)
                     /locus_tag="PLW13_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69660.1"
                     /translation="MASEARSRFCAMAVNSDKCEWVVARRAVALRGVDFFSLIQLHVA
                     TTRRSRAQCECELYAGGVEGENSV"
     gene            16146..16820
                     /locus_tag="PLW13_04500"
     CDS             16146..16820
                     /locus_tag="PLW13_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY69661.1"
                     /translation="MKKNWSKPTELHLAPRPRKQPRQSRSISLVRAIREACLRILEHE
                     GAEALTTNRIAEVAGVGVASVYQYFPNKDAILASVYEELLVRERAAITALFMDVATQS
                     SRDTLESLIDHGIDSRRRLLAIAPDFYRKYHREFQLTPGGIAQGAGPDSLRRSMTSTG
                     DMFARFGATLRVANDTSAQFIFTRGIGAIIDRAAEDAPALLEDPAFRAALKDMVARFL
                     LLPDGG"
     gene            17012..19012
                     /locus_tag="PLW13_04505"
     CDS             17012..19012
                     /locus_tag="PLW13_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HQY69662.1"
                     /translation="MQTLRYLLIPALTSTLVVSCDRHAPVDYAPGLVAEWNAYGGAPG
                     GGHYSAAEQITRDNVHVLQQAWVYRTGDKREAGKGMLDGVAVDNVPGSSWQLTPLVVG
                     DTLYGCSALNRMFALDAATGEEKWSYDPGVDTSQDMLVNCRGVSSWRSATPTGAACDH
                     RIFMGTLDARLIAVDAKTGKACEDFGERGQVDLSAGLGRFSPQDYSVTSPPAILDDLV
                     ITGAMVLDRTHDHMPSGVVRAFDVRSGKLVWYWDAIPPGTQHEFDAQGQPVYHRGTTN
                     VWSIISVDPERKLVFLPTGNTSSDFYGGNRKGVDDYYSSSVVALKGETGEVVWHFQMV
                     HHDIWDYDTPAQPTLFDFARDGKPIPALAQPTTMGHLFLLNRETGEPLFPVEERPAPQ
                     HGAVPGEYLAATQPFPTKPAPLHPGGLTADDAWGFTFWDRGECRKQIEGLLNEGIFTP
                     PSLQGSVQFPADMGGNNWGTPAIDPERGLVILNTLFMPEMLQLIPRDKCEIGKDPFTS
                     PQAGTPYCVRSMPILSPLGAPCIAPPWSTLVAVDLATGEKRWEVPLGTLEEMAPWPLS
                     RIKGAPSVGGPSVSAGGITFIAATPDYYLRAFDTVTGAELWKAKLPTGGHATPTIYRA
                     PSDDRQFVVIAAGGHFGMPQEPKGDYLIAFALPKKPEGVTHP"
     gene            complement(19120..19740)
                     /locus_tag="PLW13_04510"
     CDS             complement(19120..19740)
                     /locus_tag="PLW13_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69663.1"
                     /translation="MHFPEFFQRVPRLRVRDPLAEFLGTAQDGLLEYGYEDAVRLAGH
                     SCPTVASAYVMACRAMTMLHGQGVPERGNLRLAFADDLEQGVTGVIASVFGLLTGAAQ
                     EGGFKGLGGQFVRRGLQQFGVAIPLAVRLTRIDTGEAVDAAVDLSRVPAHGDMSLLLG
                     RCIRDEADAVERRRFAQLWQERVARILLEHWQDDEVFQLGAVKAKN"
     gene            complement(19819..20082)
                     /locus_tag="PLW13_04515"
     CDS             complement(19819..20082)
                     /locus_tag="PLW13_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQY69664.1"
                     /translation="MQTVHACNHTTAEAIYPFDARGVAKRFRHAAIFGALDSLQPGET
                     MRFCNDHDPLPLLGQLQQRYGEQVSITYRQREPGAIVIDFARL"
     gene            20410..>21816
                     /locus_tag="PLW13_04520"
     CDS             20410..>21816
                     /locus_tag="PLW13_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQY69665.1"
                     /translation="MSLLLTVLTPFLGAPLVALAAGRGRAATAWCTAGVIALAGLWLA
                     PLLPATLAGDTAIERLPWIPTAGLDLAFRLDGLGLLFVLLILGIGLLVILYAHYYLSA
                     QDSLGRLYACLMLFMGSMLGVVLSENLIQLLIFWELTSLSSFLLIAYWRRRAAARRGA
                     RMALTITGAGGLALLAGFLLLGDIVGSYELSVVLAHGEAVRADPRYTTVLALVLLGAF
                     TKSAQFPFHFWLPHAMTAPTPVSAYLHSATMVKAGVFLLARLFPVLSGTPEWTFWVGG
                     TGLVTLLLGAVVALFKHDLKGLLAYSTISHLGLIVALFGVGTPLAAVAGVFHLINHAI
                     FKAGLFMAAGIIDHECGTRDMRRVNGMWKHMPYTALLAMVAAAAMAGVPLFNGFLSKE
                     MFFAEAVAAGGQMRNGWLLPAAVTFAGIFAVAYSLRFIHDVFFNGEPVDLPRTPHEPP
                     LWMRVPVMILVTLCLLVGV"
BASE COUNT         3693 a         7642 c         7040 g         3441 t
ORIGIN      
        1 agggctacga gatgaagata ttcgacgacg atggcaacga atgtccgcca ggagtgccgg
       61 gcgagatctg ctgtcgtccc gcggctggcg gcactgcctc ggtcgacgtg gaatacctcg
      121 gcaaccccga ggcctcgaag caaaagatcc gtgccggctg gaatcgcagc ggcgacgtcg
      181 gccacagcga cgccgagggc tggctctact tcgactaccg caagggcggc ggcatccgcc
      241 acaacggcga tttcatcaac acgggtttcg tcgagaagat cattgccgag agtgcgctgg
      301 tcgacgatgt attcgtctac ggcgtgcccg cgctctcggg cgcgccgggc gagaaggacg
      361 tggtggccgc ggtggtcgcg cgcgacccgg cggcgttcga tccggccgcg ctgttcgccc
      421 actgccgcgc cgggctggag ccgaacttcg tgccgtccta tctgcaggtg gtcgacgaaa
      481 tcccgaagac cgcctcggaa aagccgcagg aacgcttcct gctggaggcg ttcagtcgcg
      541 acggcacggg cattcacgcg ctgcgctgac gcgcatccgg catgagcttc gtccacgtca
      601 cgggccacgc gtccgtactg tggacgccgg tctcgcatgc cacgatagcc gcggcctgcc
      661 acaatggttt cggggagcac gcacgcctcg tcgcctgtga agcggtgacc cacggcggtt
      721 tcaacaccat ttatcgcgtt cacatggcgg cgcacgaacc cgtcgttctg cgggtgagtc
      781 caccccggcg cgcaccgctg cttcgtcacg aacgcgaact gctggcacgc gaagcggcgg
      841 tgcacgcgcg gctcgcctcg atcggcccgg tgatcccccg catcctgcac caggacttct
      901 cctgcactac cctgccgcgg ccgtacctgt tcctggagca ccggcgcgga cagccctggg
      961 atgatgtcgc gccgcgtatc gcgcccgctc aggcggcatc gctgtggagc gagttcggca
     1021 accacgtccg gaagatccac gccatcgagg gcgaagcctt cggttcgccg ctgcgcggcg
     1081 aaggccgcgc gagtcatgcc gagtggctgc tgtcgctgtt cgatgacctg gccacggatc
     1141 tcgccgagcg caagctcctg gtgccgggac tgacggagtt ccgcatcgcg ctgcaacagc
     1201 gacgcgagca actcgaactg cacaccccgc cacgcctggt gcacggcgac ctgtggcccc
     1261 gcaatgtgct ggtcacgcgg gaacgcggcg gctggcgcct cagcgccata ctcgacgcgg
     1321 agcgcgcatt ctggagcgaa cccgccgcgg aatggatttt ctcgcacctc gagattccgc
     1381 cggccttctg ggaagcctac ggcgctgatc tctcgccggg ccggctggat ggcgacgccg
     1441 cgctgcgcac gctggtgtac cgggctcgcg gcgcgctgca gctggtgctg caatgttacc
     1501 gctcaggcgc cagcgcggca ttcgccctcg cccgcttccg cgcatgtgca cgggagtttg
     1561 cgcgccattg atcacgaaac gcgcaagccc ctgaaatccg cgacaggacc gctgcacgcg
     1621 gcgcaaggca gcgccacagc tcaggcggcc ccgctccatc cgccgccgag cgccacgtac
     1681 aactgcacca acgcctggtg cagggcgcgc gtggactgcg agaggctgag ttcgctgtcg
     1741 aacaactgcc gctcggcgtc cagcacctcg aagtaactcg tgatgccgcc cacgtagcgc
     1801 gacttcgtca cgcgcagccg ttcgcggttc gcgagcacca ccctgcggct cgccgcgcgc
     1861 tgcgcgccga gttgctcgat cgccaccagc gcgtcgctca cctcgcggaa tgcctgccgg
     1921 atggtggcct tgtactgctc caccaggatg cgctgctggc tctcggcaag tgccactccg
     1981 gagtcgagcg ccccggcggt gaacacaggc agcgccgccg ccggctgcac cgaccacgca
     2041 tggcgtccgc tatcgaacag gccgccgaca tcggggctcg ccaggccgag cgtcgttgtc
     2101 agcgacagcg agggtaaata ggccttgcgc gccgccgaga cactggcgtg tgcaccgcgc
     2161 agccgctcct cggcggcctg gatatcgggc cggcgcggca gcagcgcgga gggaatgccc
     2221 gccagcatcg tggccgggag ctcgattgcg tcgagccgca cgtgccgctc gatggacccg
     2281 ggattgcgac ccagcagcac cgcaagcgag ttctccgcct gcgccagacg ccggcgctgg
     2341 tcggccagcg ccacctccga gctcgccagc aacgactcgg cctgccgcac gtcgacgatg
     2401 gaggaaatcc cctcgtcgaa tctcgcgcgc gtcagccgca gcgactgccg tcggctctcg
     2461 agcgtgcggt ggctgatctc gagctggttg tcgaggtcca gcagatccag gtatcgagcc
     2521 gagacgtcgg ccaccaggct gatgcgcacg ccctgcgcgg cggcctcgct cgccagcacc
     2581 tcgcgacggg caacctcggt gcgatccgcc agccgccccc acaggtcgat ttcccacgcg
     2641 ggcacggcga gcgccagacc gctgctctcg ccgatcgtgt tcttcgcggg atcgaatccc
     2701 ggcgcgcgct gacgtgtgac cgaacccgag aggtcgatcc cggggaacat cgccgagcgc
     2761 tccagcccgt actgcgcgcg cgcgagctcg atccgctcca ccgcgatgcg cagatcactg
     2821 ctgccggcca gagcctcgtc gatcagtgcc gtgagttccg gcagcggcaa cacttcagcc
     2881 cagaggcggt cggcaaggct ggccgctgcg gtagcgggag acgaatccgc gccgagatgc
     2941 ggccaggtgt cgggcaccgg cacgtccgga cgcacgaaat ctgccgactg gcagccaccg
     3001 aggagcgccg cgcacagggt gatccacatc cggctcatcg cacgctctcc acctcgtcgg
     3061 cgcggatgcc gaagaaggcc gcatacagca agggcaccac caccagcgtg agacccgtgg
     3121 cgaagacgag accgaacatg atcgtgactg ccatgctgcg aaagaaggga tcgagcaaga
     3181 gcggcgccac gccgagcacc gtggtcacgg cgccgaggaa taccggtcgc gcgcggctgg
     3241 tcgccgcgtc gatgatggcc tggaagcgcg gcttgcgcga acgaatctcg acgtcgatct
     3301 ggtccaccag cacgatcgcg ttcttgatca acatgccgat caggctgaga aagcccagga
     3361 tcgccatgaa ctcgaacggc gcctggaaca gcagcagccc gatcgcgaca cccaccacgg
     3421 ccagtggcac cgtgagccag atcaccgcgg tctgccggat cgcgttgaac atcacgatca
     3481 ccgccagcac cattgccacc atgccatagg gcgcagcgct caccaggcct gcattcgcct
     3541 cggtcgacgc cttgtgctcg ccgtgccact cgagcgtgta gcccggcggc aggggaattg
     3601 cctcgatctg cgggcgcagg cgctcgaaca gtggcgccga cagcgaaccg gcgggcggat
     3661 cgcactgcgc cttgatcacc gggaagcggt tttcgcgccg gatgatcgca tcttcccagc
     3721 gcgtgtcgac gcgctcgatg aactggtcga ccggcacgaa acgccccgcc agcttgctgt
     3781 agacctgcac gttgcccaga tcctccacgc cttcgcgctc cgcggccggc gagcgcgcga
     3841 ggatcggtat cagcttttcg cgttcgcgat acacaccgat ctgctgcccc gtgtagttgc
     3901 gctccagcgc ggcagcgatg tccgagggca gcacgccggc gcgctgcgcg gcctcgacgg
     3961 agaagcgcgg acgcaccacc ggcacctgct ggcgccagtc gtcctggata gccagcgcct
     4021 cggggtcgtt cgccatgatc gccctggcct gctcggccag cccgcgcagc accagcgggt
     4081 ccggcccgcg gaaggccgct tcgatcttct tgccgccgcc gcgcccgagc atgaacttcc
     4141 acgccttgat gtcggcgctc gggaatgcct gctcgagctc gcgctgcaac tcgggaatcc
     4201 gcgcggcgat atcgccggca cgctcgacat ccaccagcaa ctggccgtag gccggattgg
     4261 gatcctcggg actgtaggtg agcatgaagc gcagggcgcc ctgccccacg aagctggtca
     4321 cgtgcgttac gccgggcttg gcgcgaacca gagcctcggc cgccgccacc atctctgccg
     4381 tggtttcgat gtcggtgccc tgcggcagat acaggtccac cacgaactgc gggcgcgcgg
     4441 aatccggcat gaaacccggc ggcacgaagc cgaaggcgat cacgccggcc gtgaacaggc
     4501 ccaacaatgc catggtgctg gcgcgccggt ggcgcagcac tgagccgagc agacgcgagt
     4561 atgcggccat cagccccccg ccccgcgcat cgcccgccac ggccttgacc cgcaggaacg
     4621 ccacacagaa cagcggcgtc agcgtgatcg cgaacaccca cgacagcagc atcgagtaga
     4681 ggatcaccca gaacagcgag cccgcgtact cgcccatatc ggtgggcgag aggccaatgg
     4741 cgctgaaggc gagtatgccg accagtgtgc cccccagcag cggccagatc gtgccctgga
     4801 ctaccgcgac cgccgcgggc acggcctcct cgccgcgctg catccgcacc aggatgccgt
     4861 cggtcacgac gatcgcgttg tccaccagca tacccagcgc gatgatcagg gcgcccagcg
     4921 agatacgctg catcgcgatg tcgctcatca gcatcgccac catcgtgccg gccaccgtca
     4981 gcaccagtac cgcgccgatg atcagaccgc tgcgcaatcc catgaagatc agcagcacca
     5041 cgaccacgat cgcgaccgcc gccaccaggt tctcgacgaa gccatccacc gccgcgcgca
     5101 ccgagtcgga ctgcagcgag accgggttga gctccatgcc cacgggacgc tggctctcga
     5161 gctccacgag ccgctgcttc accgcatcgc ccatggctac cacattgcca ccgagcacgt
     5221 tggagatacc aagcgctatc gccggccgcc cgtcgcgacg caccagcacc gtgggcggcg
     5281 tgatgtaatc acgatgtatt tccgcaatgt cctgcaatcg caccaggccg ctcgtggcgt
     5341 cgcccgcgac caccaggtcc ttcagcgtgt ctatggactc caccgtgccc tcggggttga
     5401 tctgcagccg cagcgactcc gcgcgcacat taccggcggg actgatcacg ttctgcgcgc
     5461 gcagcgtcgc gtacacctga tcgagcggaa tgccaaactg cgccgcgcgc gcacgcgaga
     5521 tctcgacgaa tatcgcctcg gtgcgctcgc cgagcagcgc cacgcgcgcc accccgggca
     5581 ccagcaccag ttcgcggcgc agcaggtcga ggtagtcgta gagctggcgc ggcgtgtagc
     5641 cctcgccggt cacggcatag aacagcgcgt acacatcgcc gaaatcgccg ttgaccacgg
     5701 gctgagccgc accgggcggc agcgcgcgcg agacgtccgc gatcttgccg cgcagcttgt
     5761 cccacacctg gtcgagctcg gcctgcgtgc gcgagaactc cagcttcgcc tcgaccttca
     5821 ccagcgactc gccggcacgc gagaccgaga tgacttcctc gacttcctgc atctgctgca
     5881 cggcgccttc gaccaggtcg gtgatttcct cggccacttg cgccggcgag gcgcccggat
     5941 acgcggtcag cacgacggcc tggcggatca cgaaatcggg gtcctcgaaa cgcccgagcc
     6001 gctcgtacgc caggtagcca cccacgagca ccagcgcggc gagcacccac gcgaccacct
     6061 ccttgtggag cgtgtacccg gcgacgttca tgccggatcg gctcccgtcg ccgacagcgg
     6121 acgcacctgc atgccggcgc gcaccgcgct gacgcccgcg gcgatgatgc gctcaccctg
     6181 cgtcaggccg gttgcgatcg tgatatcgcc accgcggatg gcgcccgtgc gaacctcgcg
     6241 gcgctcggcc ctggattgcg cgtcgagcac ccacacaaag gtggcgccgc tcgcatcggt
     6301 agcgaccgcc gtcaagggaa tggatacggt tgcgccgggt tcagccgccg cgccgacaaa
     6361 cggcagcacg gtgaccgaca tgcccgggaa cacgttcatg tcaggcggca gtgcacccag
     6421 gctcagcacg atctcgtagg tctgcgtctg cggatccgcg tcggtggcaa aggatttgag
     6481 cttcagcggt atggagcgtg tgtcttcccc ctcgaatatc gccgccgcgg cctgttgctc
     6541 cgtggcggtg cgcacgacgc gcgccggcac gttgatgacc acctcgagcg cgtccaggtc
     6601 ctggaggtcc gcgatgggct gcttggcctg cacgttgctg aaggtttcca gccgccggcg
     6661 cgtgagcacg ccggcaaatg gcgcacggat cacggtatcc gcgagctccc ggttcgcggt
     6721 ggcaagattc gtgcgcgcca cctcgagcct gctgcgccgt tcgtcgacct ccgagggtgc
     6781 taccgcgcgc tcggtttccc agagacgctg gtagcgctcg agatcggcct tcgcccgatc
     6841 gaattccgcc ccggccgccg tgacgcgcgc acggaacacc gcatcgtcga gccgcgccac
     6901 cacctgcccc gcctgcacat gactgcccgc gattggcacg aactcggcca cgaagcccgg
     6961 cacgtcgaac gacagctcgg cacgtcgctc ggcgcgtatg cggccgggaa agcgcagctt
     7021 cccgccagca gcggatgatc gcacctcgtg gatcagcgca ggtcttgcgg cagcggtggc
     7081 ttcgagcggt gcttgcgatt cgccgcaacc cgcgaggcag aagattgccg cgaaaacgag
     7141 tgcggcgcgc acgcgccccg gagcgcaggc cttggcgggg ccgccagtcg cgtgcaggag
     7201 ttcagccatc tcgggatccc cgcgcaatcg gttgctaccg ggacttcccg ggatgccgtg
     7261 cgcccagcag ttggattcag tcgtcgtcgt tggcttcgat gatggcggcg gtcaccccga
     7321 ccagtgcggc gccgacgaga gcacccttga ggaacgcgtg atcgtcgaca cggtcgcgac
     7381 gatcgcgcca gcgctcgtcc tcccagcggc tgtcgcgcat ggcgccgcga atttcacgac
     7441 gcgcctcgcg cttctcgcgt tccacttcgc ggtagccctc gcgcatctcg cgacgggcgc
     7501 actcgcgcgt ttcgcagcgg cgcagcgcgc gctgcgcctc gcgcttctcg cgctcgacgt
     7561 tgcgcgtgcc ttcacgcatc tccttgcgca cttgccagcg gtcggatcgg gcttcggcgg
     7621 gcagcaccgc cgcgagcagc aagagaccga gcgccaaggc tccggcaggc agccagcgtg
     7681 ctgtcatggt gcgaccctgc cactgctcgc gcggaacgcc tgcacccgcc gttcatattg
     7741 cgccaggact tccttgcatt ccgcgacctc caatgtcagt gaggtgccat gcccgaacgc
     7801 agcgccgaac atgaaggcac gatcggtgcc gagcaggcgg ccgatcgcgc cgtgcagatc
     7861 cactgcctcg gactcgagcg ccgggcgctg cgcctctacc acgcagccgc gcgcaaggcc
     7921 cgcaagataa ccgaaacgct ggagtccctc gtcgagttgc tcctcggggt ctggcgccgc
     7981 ggaagcggtc acaggactcg ccggatccgc cgcctgcaga ccctgaagac cgagacaaaa
     8041 agacactgca gcaagcgtca caaaaatgtg gcggatattc attcgggagt cctttgaaca
     8101 tgcgtgatcg gcaagtgctt gcgccgctgc attctatgcc agcaattgcg cggtgcggcg
     8161 cctggtgcaa accgagccgc ccattatggg ccttgcgccc tgaccctgac gcggacccgg
     8221 acgcgccgag gatcaacgcg gaccgtgtcg cgccggggaa tcgtgcagcg cgttgatcag
     8281 caccgccgtg gagaagccga acatcatcag gccgttggct gcctgcaacg gcgccagcag
     8341 gcgcgccgtg ccgatgagag tgatatcgcc gaagccgagc gacgtgaacg tgacgcccga
     8401 aaagtacagc gccgtctcga aatcgccgaa ctgcccgagc gccaagtaca ccagcgccca
     8461 ggcgatcatc tgcacgatgc tgccgatgct gagcaccagc atcagcaagg ccatgctgcg
     8521 gaaggtgatg ggcgcccgcc gccgggcgcc cgaggcggct gcaccaccga tgaaatactt
     8581 gagcgcaacc agcgccgtaa gcgattgcag gccgagacat gccgtcgcca cgccgaggcc
     8641 aaccgcaatt atcgtgatca tcggccgccc cgctacaccg gccgttccct gccggcttca
     8701 gacagcaggc gctgcacctc ggcggcggtc tgcagcaggt tcttgaccat gtcgcgcgtg
     8761 aactcgtagg catcggagcc gatggcatcg gtgcggaaaa gccagctccc atcgggcaag
     8821 cgttccaccc gaccctgttc gcgcaggcgc tcgagcttgc ggcgcacggt ttcgcgcggc
     8881 aaaccggtga cctgcaccac atcggtgagc cgcaccggat gcagcgccag cagcatggca
     8941 tcgccgacgg tcggtgcctc cagcggatcc atgccgggca gtgcaatgtt catcgcattc
     9001 atgtgcgcca gcgttgccca gacgaagacc gactcgaggt cagcatccag cgcacggctc
     9061 acccggcgca tgtgctgggc caggtgccga ttcatcgcaa acgcgagcag cccgaacgcg
     9121 cgtctgaaac tttcttccga gtcagccatg gcatcgtcct gccgttgcgc ccaaagtggg
     9181 cattattcaa ggcgagcgac gcgctgccta cagactgacg cccagattgg gcgggagtgg
     9241 agcgctcatg attggtgggc atgccgggca atcgggactt cggtctccac acgtgaggga
     9301 ctgaatctcc aggcgagcca taccaggacc cattctgggc aaagcgggac ccggctcgta
     9361 acctgcgtcg ggcatactct ggcgctcatt ttcgcaaaca agattagcag cgcatgagcc
     9421 acgagaaccc cggcaatctc atgagccgtc gcgagatgct cgccctgggc gccagcgccc
     9481 tgacactcgc cgcggcgcca gcgttcggcc tcgcagcggc agatgcggcg cccggcccgc
     9541 cgtccggcgg cgaccgggtg attctctcga acgaggattc ctgcacgctg accgtgatcg
     9601 acccgtccac gcagcgcgtc aagcacacca tcaacttgac gagcttcgat gaagatccgc
     9661 ggccgccgtt tcgcttcgtg accggcgaca tggtcccgtc gcatggcgcg atgatccaga
     9721 agccgctcta tcacggcgcg atctccgtgc acggttgcgc gccgtcaccg gattcgaggt
     9781 tgctggcgac ggtcggccgc ggttccggca atctgtatct gatcgacaca cagagcctcg
     9841 ctgtggtcgg caaccggccg aacccgcgcg caggcccgac caccaacgcc gagactctca
     9901 cctccggcgt cctcgtcgga cgcgagccgc acgaacccac gttcaccccc acgggccgcg
     9961 agatctgggt cacgctgcgg ggcgaagacc gtattgccgt gctcgacgtc gatgcgattc
    10021 ccgcggaatc gagcggacag acggcgcgcg gcgcctccat ccgggggttc gtccggacgc
    10081 cgcccggccc cgcgatggcc tggttcgcgg atggcggccg caccgcgttc gtggtctcgc
    10141 agaagacttc gagcatcgag gtgatggacg ttggttacga cgcttcggga tattccactg
    10201 tcggcatgcg aaagacactc gacatcagcg cgcaggatcc gttcgggttc tcgcccttcc
    10261 tgcgccgctc gccggacggt cgagagatct gggccgtgca caagctcgcc gatcgggtgt
    10321 ctgtgctgtc gacgggtggc gtcgtcgagg cacgggttgt agagacgctc gcaatcggcg
    10381 acaaggcgcg ccccaatcat ctcgagttcg tggagaacgc caacggccgc gcggtctacg
    10441 tgagctgcgc gcggctggac acggcgggca acggcatcgc cagtccgata atgatcatcg
    10501 accgctcggc accttacggc aagcggcgaa tcgtcgggca gttcttcagc cagggccgcg
    10561 aagcgcacgg catctggtgc gacccgtcag cgtcgatgct gttcatcgcg cacgaactgg
    10621 acgaacttcc gcagcagccg aacgcggggc agaccgtgtg caccgtattc gacgtgaagg
    10681 acccgttcgc gccgcgtttc atgacgcaga ttccgctggg cgacctcgcg ctgccctcgg
    10741 ggcggctgcg caacaagaag agcgtcaacc tggtctacgt gcgcccgggc atgccaagcg
    10801 ccacggcagc gtgagagaca gacgatgaac gactacgcga catacacgcg cgagatgcat
    10861 gcctccatgc agcagatgat gaaggatatg catcgggatc cgccatctgg cgaccctgac
    10921 atcgatttcc tcgcgatgat gatcccgcac caccagggcg cggtcgatat ggcacggctc
    10981 gtgatcgcgg cgggccggga tccgcaggtt cgcgtccttg ccgaggcgat catggcatcg
    11041 cagctgtccg agatcgaagg catgcgcggc cggctggccg ccctgcgccg tggtggcagc
    11101 cccgatgacg ggttcccgtc gcccaacggc actcggggct gataccactc gcaacgagaa
    11161 tccgcgacgc tcaggacccg gcgcgcggat cgagtcgcaa acgtccccat gtcccacccc
    11221 acatcaggcg accgtcggga tcaagcacca cgggcgcgaa catggtgttg tagcgcgcac
    11281 gcgggagcac ccagtgaaag cgtgacgtgc cggtgcgcca gtcgagcgcc tcgagcgtcc
    11341 agtgtccgtc gcgcgcgcct tcggtataga ccatgccgct gcccgcgctg atcatcggaa
    11401 cggagttcgg cgaactcacc tcgcggttca cccaggcttc gtccagcgta cgcgtcgcgg
    11461 cattccactc gaacttctgc acgccgagcg gtctgtgctc gtcatgtgtg gccagcaagc
    11521 cgaccagcac cgggctgaag ctcttcggca gataccaggg gatatgcggc ggctcgttgt
    11581 tcacgaccag cgcgccatga cccagaacac ccaccgactg ttccgactgc accgccgcca
    11641 ggttcgaatc gcccatggtc gcgggctggc tcgcggcgat gcgcctcgac aggccaggca
    11701 gaccgttcca gtccgcgggg atcgcgtcac gccagaacaa cgtcaggttc atcagcgcgt
    11761 caccgtcggt gatgaccacg aaacgatcgt cgtcgccgga acccatcagc gtcggcgtcg
    11821 atcccgagcc attaccggcg ctgttccgat acggttcacg ccacgcgccg tcggcctcgt
    11881 cctgcgacca gccctagccg gtccagcgaa tgcagtgcat gaagtcgcgc gaaacgatat
    11941 acaccttgtt gtcggagccg atggcaaagg aattacgcac ccagcccgag agcgtatcgc
    12001 tggaatcccc gtcgttggcg ggagccccgt ctagatgcgc caggtagtga cgactgaagt
    12061 cgcgtgccac cgcaacgacg tcgcccttat cggtaactcc gaccagccag ccgtcgtaag
    12121 tgaagttcag accgaccagg atcccccgcg cttgcggcgg catctcgaag cgccgcttcc
    12181 gctcgatgcg gctcccggcg ttaccgcgtt gcgcatcgcc atagacttcc acgccaccat
    12241 tacgcagcac gtagaattcg ttgtcgcggt cgaccagcgg ataaacgccc tgcgactctc
    12301 gcagcaacat catctcgcgc actccgtacc acaccgccag gggtccgctg gatgtctcga
    12361 tcgcccgctc gcgctgctcc gcccaggcag gcgtgtaggg cgagttctcg cgcagcatca
    12421 gtgtgtcgac gatgtcgaag ctcgcgtagt cctgcttgac cagacggtcc gcgcccaccg
    12481 accagatcac gcgccgcccg tccgcatacg gcccgctgat caccgggatc aggtgcgccg
    12541 gccccatgaa gcgataggcg atatcggcat cggtcaatgt ccggttgctg ctcaccggcc
    12601 ccgcgaccgc gacagacccg ggttgaccgg acgcggcgtg cgtgatcggg taggtggaat
    12661 ccgcgagcca gggattacgc ggttcgaccg tggcggaatc cgccatgacg cccagcggca
    12721 acagggcgca cagcgtcacg acggtacgct ggcgaaggtg aagggagaac gactctggca
    12781 tagtcttcgg ttcccggggg cgaatggatt caggtgagga ggcgcccggg cagcaggccg
    12841 gtggtgacgc cgtcgcacac ggtgggcacg ccggcgacca gcgtcatgac gtagccgtcg
    12901 gcgtcctgca ccaggcggcc accgcctgcc ggcaggtcgt actcccaagc gggcatgcgc
    12961 aacgcgagcc gctcgaggtc gatcaggttc aggtccgcgc gcttgccggg cgcgatcagc
    13021 ccgcggtcga tgaaacccca tgcctcggcg ggccgctgcg tcagctgctg caccagccac
    13081 tcgagcgcga agcccttgcc gtccggctgt tcgcgccact gcgtgagcac gaaagtgggc
    13141 atgccggcat cgctcagcac cgcgcaatgc gcgccgccgt cggccaggcc aatgacagtc
    13201 agctccgact ggatcatttc gcgcagcacg gcgggcgatg cctcggcgta gttgcgcacc
    13261 ggcgtgtaga ggaaatgccg cccctcgccc tccagcagcc aatcgtaggc cagaaactcc
    13321 ggcgcggtcg cgctgcgcgc ggcctgcacg gcaaccgagc tctcgcgcgg gggcaggtac
    13381 ggcagggtgg cgtcgaagcg gaagatgttg ttccagtcac atagcatcgc cgagggaagc
    13441 aacgaaagat cgagctccgc gagtatccgc cgtttcgtgt ccggttcgcg caggcgtgcc
    13501 gcctgctcgt ccagcggcaa gcccatcagc gcccgatacg acgccgacac cgcgaggaaa
    13561 ttcacgcggt tcgacaatcc catcagcacg ctgatcggcc gcgctgccac ctgcgcgtgg
    13621 atatcgagac cctcggcacg cgcgcccgcg atcagctcga ggatgcgccg ccacgcatcg
    13681 ggctgttcgg gcgtctgcgt cagcgccacc gacacctgcc ggccgctctg tgccgcgatc
    13741 tcgcggatca gcgcgaactc gtgctcgagc gggctgaagt cggaaacgat ttcgaagatg
    13801 ccgcgcccgc tcgttcgcat cgcgcgcgcg atgccgagca gttccgcggg ctttgcgctc
    13861 aggctcggcg tgggcttgcc gtcactggag cgatgcacca ccgtgcgcga ggtcgagaag
    13921 ccgatcgcac ccagcgcgag cgcctcgctg accaactccc ccatcgcacg gatttccgct
    13981 tccgtcggat gcatcgtcgg atcagcgccg cgcgagccca tgacccaggt gcgcaacggg
    14041 ccgtgcggga tctgcgccgc cacgtcgagc gcaaagcgct tccggcgcag cgccgcgagg
    14101 tattgcggaa agctttccca ctgccagtcg ataccctccg ccagcacggt gcccggaatg
    14161 tcctccacgc cctccatgag gcggatcagc cagtcgcgct cgcctgctcg caccggcgcg
    14221 aagcccacgc cgcaattgcc catcacgacc gtggtaacac cgtgcaggca ggaagggctc
    14281 aggtacggat cccacaccgc ctgtccgtcg taatgcgtgt ggatatcgac gaagccgggc
    14341 gtcacgacga gtccgcgtgc atcgatctcc tgcgcgccgg cttgcgcgat cgcgccgatc
    14401 acctcgatga cgccgttcct gatcccgatg tcggcaatgc gcgcgcttgc accgctgccg
    14461 tcgaccacca ggccgccgcg tacgatcgtg tccagcatga gactactccg ggcgcgcggc
    14521 gcctcagcgc gccggcgctt cttcagcgag aatgaaattg accggcgccg ccatgcccgg
    14581 cagatccgcg agccgtccgg cgttcttcac gctgctggcg ttgtagccat gcacgccctc
    14641 gtcgcccgcg gcaacgggta ccagctccgc cttgccctgt tcgatagtcg ccagatcgat
    14701 gtcgtagagc gcggcaacgt tgcggaacag gatcttctcg agcatctcgc ggggcatgcc
    14761 gtcgcactgg ctcgccagca gcggctgcga ccacggccag gtcgagtccg agtgcggata
    14821 gtcattggcc cacatcacgc gctccggatt gatcatcccg gccttcgcag caccgaaggc
    14881 cgtccagtcg tcctggaagg tgacgtagat gttctccgag acgtattccg atggcttgcg
    14941 ctgcaactcc ttgcccggcg tgatgtggtg acgatggcgc aggtagccat gatccatgcg
    15001 gtacatatag tgtggtaccc agcccgcgtc cgcctcgacg ctgaccaccc tgagatccgg
    15061 atgacggtcg aacacaccgc cgaataccag catgcccatc acgtcctggc aggcgcgcat
    15121 ggtgttcatg aagccgttca gcgccgaacc gcgatactgc ttgccacgcg aggtgaggat
    15181 gtggaacgaa accggcaacc gcatctcgac ggccgcacgg aagaagtcgt cgtagatcgg
    15241 gctgtcatag tcctcctgcg cgggttcacc cggcagcatc acgccgcgca tgccgaggcg
    15301 gcgcatgtcc tcgaggtcgg cgatcgcctc gagcggatcg tgcatcgcga tctgtccgat
    15361 gccgagcaga cggttcggcg cgctcgcgca gtactccgcg agccatgcgt tgtacgcccg
    15421 gaagcaggca cgcttcagtt cgaagtccgg gtggttgcag aacatcatgc ccacggtggg
    15481 gtaaatcagt tccgccgaca cgccgtcctg gtcctgctcc agcagccggg ccacgggatc
    15541 ccagccgccc cgatgcatgt cctcccattt cacgccttcc gtgcgaatct cgatcgggtc
    15601 cttgccggcc gcggcaatga gtcccacggg gatcggcggc aggggatcgc ccatcacgaa
    15661 catgtcgcca cgctcgccgt tgcgcaccag atgcggcacg cggtcgcgat acttcgtatc
    15721 cacgcgggac acgtacaggt ccggtggttc ggtgatgtgc gagtcggcgg aaatcagtgt
    15781 ctgtttcacg caggggtctc cggttccggt catttgggca tcaaaccgaa ttctcgccct
    15841 cgacgccgcc ggcgtacaac tcgcattcgc actgcgcgcg ggatcgccgt gtcgtcgcca
    15901 catgtaattg aatcaaagag aaaaaatcca cgccacgaag ggcaacggca cgccgcgcga
    15961 cgacccattc acatttgtcg gagttcaccg ccatcgcgca gaaccgcgac cgggcttcgc
    16021 tcgccatgca gcaacaaccg cccgatggcc aactgccgca ggcatgggcg caagatcgga
    16081 ctaaactgcg cgatggcggg ggcacccgcc cggagtgcct ctgacaacga ccggcgatcc
    16141 cgcagatgaa gaagaactgg agcaagccca ccgaactgca cctcgcaccg cggccgcgca
    16201 agcagccccg acagagccgt tcgatcagtc tcgtgcgcgc catacgagag gcctgcctgc
    16261 ggattctcga gcatgagggt gccgaggcgc tgacgaccaa ccgcatcgcc gaggtggccg
    16321 gcgtgggcgt ggcctcggtg taccagtatt tccccaacaa ggacgcaatc ctcgccagcg
    16381 tctacgaaga gttgctcgtg cgcgagcgcg cggcgatcac ggcgctattc atggacgtgg
    16441 cgacacaaag cagtcgcgac acgctggaga gcctgataga ccacggcatc gattcgcggc
    16501 gtcgcttgct cgccatcgcc ccggacttct accgcaagta ccaccgcgag ttccagctca
    16561 cgcccggcgg catcgcgcag ggcgcgggac cggacagcct gcgccgctcc atgacctcca
    16621 ccggcgacat gttcgcccgc ttcggcgcga cgctgcgcgt agccaacgac accagcgcgc
    16681 aattcatctt cacgcgcggc atcggcgcga tcatcgaccg ggccgcggaa gacgcgcccg
    16741 ccctgctcga ggaccccgca ttcagggcgg cgttaaagga catggtcgcg cgcttcctgc
    16801 tgctgcccga cggcggttga agcgccttgc cgctcacccg gcagcccggc gcccattccg
    16861 ggcaacatga acaattcgaa agttgccaat gcgacggccg ctcttcagtg ttcgcagccg
    16921 cgggccgtcg cgcaccgcgt cgtgtgcacg gtgacacggc catggtcgcc ggcgcatcga
    16981 cccgacccga cccaagcgca aggacactcc gttgcaaaca ctccgctacc tgctcattcc
    17041 cgcgctgacg agcaccctgg tcgtttcctg cgatcgtcac gcgccggtcg actacgcgcc
    17101 cgggctggtg gcggaatgga acgcctacgg gggcgcgccg ggaggtggcc attactcggc
    17161 ggcagaacag atcacgcgcg acaatgtgca cgtgctgcaa caggcatggg tataccgcac
    17221 cggcgacaag cgcgaagccg gcaaggggat gctcgacggc gtcgcggtcg acaacgtacc
    17281 gggcagcagc tggcagctca cgccgctcgt ggtcggggac acgctctacg gctgcagcgc
    17341 gctcaaccgg atgttcgcgc tcgacgccgc cacgggcgag gagaagtgga gctatgaccc
    17401 gggcgtggac acttcgcagg acatgctcgt caactgccga ggcgtgagca gctggcggag
    17461 cgccacgccg acgggcgcgg cctgcgatca ccggatcttc atggggacgc tggatgcgcg
    17521 gctgatcgcg gtcgacgcga agaccggcaa ggcctgcgag gacttcggcg agcgcggcca
    17581 ggtggacctg agcgcgggcc tcggcaggtt ttcgccgcag gactactccg tcacgtcccc
    17641 accggcgatt ctcgacgacc tcgtgatcac cggcgcgatg gtgctggacc gcacgcacga
    17701 ccacatgccc agcggcgtgg tgcgcgcctt cgacgtacgc tcgggcaaac tggtgtggta
    17761 ctgggatgcg atcccgcccg gcacccagca tgagttcgat gcgcagggcc agccggtcta
    17821 tcaccgcggc accaccaacg tgtggtcgat catctcggtc gatcccgagc gcaaactcgt
    17881 gttcctgccc accggcaaca cctcgagcga cttctacggc ggtaaccgca agggcgtgga
    17941 cgactattac tcgagctccg tggtcgcgtt gaagggagag acgggcgagg tcgtctggca
    18001 cttccagatg gtgcatcacg acatctggga ctacgacacc ccggcacagc cgacgctgtt
    18061 cgacttcgcg cgcgacggca agccgatccc cgcgctggcg cagccgacca cgatggggca
    18121 cctgttcctg ctgaaccgtg aaacgggcga accgctgttt cccgtggagg agcgacctgc
    18181 gccgcagcac ggggcagtgc cgggtgaata tctcgccgcc acacaaccgt ttcccacgaa
    18241 acccgcgccg ctgcaccccg gcggactcac ggcggacgac gcctgggggt tcacgttctg
    18301 ggaccggggc gagtgccgca agcagatcga gggcctcctg aacgaaggca tcttcacacc
    18361 accgtcgctg cagggctccg tgcagttccc ggccgacatg ggcggcaaca actggggcac
    18421 gccggcgatc gaccccgaac gcggcctcgt catcctgaac acactgttca tgccggagat
    18481 gctgcaactg ataccgcgcg acaagtgcga gatcggcaag gatcccttca cctcgccgca
    18541 ggccgggacg ccgtattgcg tgcgcagcat gccgatactg tccccgctcg gggcgccgtg
    18601 catcgcgccg ccatggagca cgctggtcgc cgtggacctc gctaccggcg agaagcgctg
    18661 ggaagtgccg ctcggcacgc tggaggagat ggcgccgtgg cccctctcgc gcatcaaggg
    18721 cgccccgagc gtcggtggcc ccagcgtgag cgccggaggc atcaccttca ttgccgccac
    18781 gccggactac taccttcgcg ccttcgacac cgtcaccggc gccgaactgt ggaaggcgaa
    18841 acttcccacc ggaggacatg ccacgccgac gatctatcgc gccccgagtg acgatcgaca
    18901 gttcgtggtg atcgccgccg gcggccactt cggcatgccg caggaaccca agggcgacta
    18961 cctgatcgcg ttcgcgctgc cgaagaagcc ggagggtgtc acacaccctt gactgcaccg
    19021 ctgcccgtcg cgcgcgtgcc ggggacgcga gccatgctta cgcgaattgc cgtcacgcgg
    19081 cgccatcgcg cggcgtcgaa tgcggtggct cccgggctct cagttcttcg ctttcaccgc
    19141 acccagttga aacacctcgt catcctgcca gtgttcgagc aggatccgag caacccgctc
    19201 ctgccacaac tgggcgaagc gccgacgctc gacggcatcc gcttcgtcgc ggatacaacg
    19261 tcccaggagc agcgacatgt ccccatgcgc gggtacgcgc gacagatcca ccgcggcgtc
    19321 gaccgcctcg ccggtgtcga tgcgcgtcag gcgcaccgcg agcggtatcg cgacaccgaa
    19381 ctgctgcagt ccgcgacgca cgaactgacc accgaggccc ttgaagcctc cttcctgcgc
    19441 cgcaccggtg agcagaccga acacgctggc gatcacgccc gtcaccccct gctcgagatc
    19501 gtcggcgaac gcgagcctga ggttgccgcg ttcgggcacg ccctgcccgt gcagcatcgt
    19561 catcgcgcgg cacgccatga catacgccga agccacggta gggcacgagt ggccggcaag
    19621 ccgcacggcg tcctcgtagc cgtattcgag caggccatcc tgcgcggtac cgagaaactc
    19681 ggccagggga tcacgcaccc gcaagcgcgg cacgcgctgg aagaactcgg gaaagtgcat
    19741 gtgcttgctg cctcgagcct gccgggcgcc gggtgacggg atcgaatgag cccggcgccc
    19801 ggcacccggc gcggcgtgtc agagcctggc gaagtcgatc acgatggcgc ccggttcgcg
    19861 ctgccggtag gtgatggaca cctgctcgcc gtagcgctgc tgcaactggc ccaatagcgg
    19921 cagcggatca tggtcgttgc agaatcgcat cgtttcaccc ggctgcagcg aatcgagcgc
    19981 gccgaagatc gccgcgtggc ggaaacgctt ggcaacgccg cgggcgtcga aggggtagat
    20041 cgcctcggcg gtggtgtggt tgcaggcgtg gacggtttgc atgcggactc ctgatactgt
    20101 ggataaatcc gactgatttt gtcggataaa agcagtatgc tggccaccgg ttggtaattc
    20161 cttgatctgg atcggcatta gtcgcaactg cagggctggc ggtagtcagg cgcgcgatgc
    20221 ggattcgtgc ccgcagcgcc cgaccggtcg cgaagctgtc aacggcgcgt cccggcctgt
    20281 aaagtgccgg ctcgatagtc tcgcgaggcc ggagattttg cgtacacgat ccaatgcacg
    20341 gcgcccgcgc gttgccggcg cgtggggcag cgccgatctg ccgcccggtg cgcgggggcc
    20401 atcggcacga tgagcctgct gctcacggtg ctgacgccct tcctcggcgc accgctggtc
    20461 gccctggccg ctggccgcgg tcgcgccgcg accgcctggt gtaccgccgg cgtgatcgcg
    20521 ctggcgggcc tgtggctggc accgctgcta ccggcaaccc ttgccgggga caccgcgatc
    20581 gagcgactgc cctggatccc gacggcgggt ctggacctcg cctttcgtct cgacggtctc
    20641 ggcctgctgt tcgtgctgct gatcctcggc atcggcctgc tggtcatcct ctatgcccat
    20701 tactacctgt ccgcacagga cagcctcggg cggctctacg cctgcctgat gctcttcatg
    20761 ggctcgatgc tgggcgtcgt gctgtcggag aacctgatcc agctgctgat cttctgggag
    20821 ctcaccagcc tgtcgtcgtt cctgctgatc gcgtactggc gcaggcgcgc ggcggcgcgt
    20881 cgcggtgcgc gcatggcgct cacgatcacg ggcgcgggcg gcctggcgct gctggcgggc
    20941 ttcctgctgc tcggtgatat cgtcggcagc tacgagcttt ccgtggtgct cgcgcatggc
    21001 gaggcggtgc gcgccgaccc gcgctacacc acggtgctcg cgctggtgct gctcggcgcg
    21061 ttcaccaagt cggcgcagtt tccgttccac ttctggctgc cgcacgccat gacggcgccc
    21121 acgccggtct cggcctacct gcattccgcc accatggtga aggccggcgt gttcctgctg
    21181 gcccgcctgt ttccggtgct ctcgggaacg ccggaatgga cgttctgggt cggcggcacc
    21241 gggctggtga cccttctgct cggcgcggtc gtcgcgctgt tcaagcacga tctcaagggc
    21301 ctgctcgctt attcaaccat cagccacctc ggcctcatcg tcgccctgtt cggcgtcggt
    21361 accccgctgg cggcggtggc cggcgtgttc catctcatca accacgccat cttcaaggcg
    21421 gggctgttca tggccgccgg catcatcgac cacgagtgcg gcacgcgtga catgcggcgc
    21481 gtcaacggca tgtggaagca catgccgtac acggccctgc tggcgatggt ggcggcggcg
    21541 gcgatggccg gcgtgcccct gttcaacggc ttcctgtcca aggaaatgtt cttcgccgag
    21601 gccgtcgcgg ccggcggcca gatgcgcaac ggctggctgc tgcccgccgc cgtcaccttc
    21661 gcgggcatct ttgccgtggc ctattcgctg cgcttcatcc acgacgtgtt cttcaacggc
    21721 gagcccgtcg acctgccgag gacgccgcac gaaccgccgc tgtggatgcg ggtcccggtg
    21781 atgatcctgg tgacgctgtg cctgctggtc ggcgtg
//
LOCUS       DAORUQ010000030        21803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_1989_length_21803_cov_6.255610, whole genome shotgun sequence.
ACCESSION   DAORUQ010000030 DAORUQ010000000
VERSION     DAORUQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21803
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_21803_cov_6.255610"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLW13_04525"
     CDS             <1..226
                     /locus_tag="PLW13_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69666.1"
                     /translation="TTMLAGMGEHLLSEYYIVRAPALREMELDEEEMTEWISVMFYRA
                     LFLENPPAERLKHAALVTHLRRSAGVPGRI"
     gene            261..1220
                     /locus_tag="PLW13_04530"
     CDS             261..1220
                     /locus_tag="PLW13_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY69667.1"
                     /translation="MSETDETIQSYVGRPPCGPYNSWDEVNAPMIRQLCEALGDENPV
                     YIDPAFAAQSVHGGLVAPPTMLQVWTMRGVGGKWAPGSDSREPFEVFEYLKTIGFPAV
                     VAVNCEQNYYRYVKPGDKLHHTSSIESISPQKTTALGTGYFVTELEEFFDQNGEKVGD
                     MRFRVFLYRAPDRAQEAAPAGEAAKPAKIKRFRPIENHDTKFFWEGVRQGQLLIQRCK
                     ACNTLRHPPGPMCPSCQSLEWDTVSSSGKGTIYSFVVMHHPQIPPFDYPNTILLVELE
                     EGTRLVSQVIDTHPDEVQIGAAVEVVFREVEEGCTLPLFRVVK"
     gene            1237..2373
                     /locus_tag="PLW13_04535"
     CDS             1237..2373
                     /locus_tag="PLW13_04535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69668.1"
                     /translation="MDFTRSEESQSIQDLAAQIFADQAGDEALKRFDRHEIAYHTGLW
                     KLLAEAGLIGVALAEEHGGSGLGFTELCAVFEEQGRHVAALPLLATVVLGALPIAAFG
                     SEAQRTQWLPGVARGELLLSGALCCPEVGGHADRLPRASADGAGWRLDGTLDRVPYSL
                     EAAAIVVPAQGDDGAMHVFLVPAKAAGLERERQRGTNHEPVDTLAFTGVKVASADLLG
                     KPGEGACIAEWIGTRAATALGFMQLGVLSDALRRTAAHTVERKQFGKPVAGFQAVAHR
                     AADAYIDVEALRSTAWQAAWRMSESLPAEAAVASVAWWACEAGHRVGHACQHLHGGAG
                     ADLDAPIHRYFLWARQVELCLGGSNRILARFGASLSNQTIEIGL"
     gene            2370..2795
                     /locus_tag="PLW13_04540"
     CDS             2370..2795
                     /locus_tag="PLW13_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQY69669.1"
                     /translation="MSIQDRTTLKFADVRVGDELPPLGIELTTRLIVSTALATRDFQD
                     VHHDKPGAQALGSPDIFMNIITTNGFVGRYVTDWAGPEALLRSVKIRLGAPNYPGDTM
                     VIKGKVTEVHSDAKTVDIEVGGKNGLGYHVTGTVTVALP"
     gene            2806..3990
                     /locus_tag="PLW13_04545"
     CDS             2806..3990
                     /locus_tag="PLW13_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HQY69670.1"
                     /translation="MSDNKFLSGRTAVVGLGATEFSKDSGRSEIQLGVEAISMALADA
                     GLSIDEVDGMATYTMDNNPEIELYRLLGGKELKFFSRTHYGGGAACGPLLHAAMAVAT
                     GVCKAVVVYRAMNERSGFRFGQGGLISNDPNSFETVHFGWYMPFGLSTPASWVAMCAK
                     RYLHTHGAEPDDFGRVAVSTRDFAATNPAAFFYQKPITLDDYRKSRWIAEPLRLFDCC
                     QESDGAVAMVITSIERARDLKQKPVVIRSAAQGAAGDFQMMTGFYRPDLTAIPEMGLV
                     ARQLYGMAGVGPQDIQTAVLYDHFTPFVLPQLEEFGFCGRGEAKDFIRAGHISRGGKL
                     PVNTHGGQIGEAYIHGLNGVAEGVRQVRGTAVNQTPGVEHALVTAGTGVPTSGVILGV
                     DR"
     gene            4087..5019
                     /locus_tag="PLW13_04550"
     CDS             4087..5019
                     /locus_tag="PLW13_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY69671.1"
                     /translation="MRKLLLLFGVLCSLHATALTLDMPSPGEDIVGEVMTVTVADYEH
                     TINTYAEAYGVGFRQLVAANPGVNPWVPGEGTELVLPLQFILPPGEREGIVINLAEMR
                     MYHYQPDGGTVETYPIGIGREGWETPLVAGAKVLSVIKDPTWVPPPSIRADHLAMGHR
                     LPAVVPPGPDNPMGPWAVQLSTRGYYIHGTNKELGIGMRVSAGCIRMYNKDVEQFARR
                     VAKGTTVRIVNAPVKVGWKQGELYVEVHEALEEQREVHVPEADVADAIHLARRLSSTP
                     VEINWVGAKAAALRRSGMPVRVSVDDSAGIASSR"
     gene            complement(5171..5437)
                     /locus_tag="PLW13_04555"
     CDS             complement(5171..5437)
                     /locus_tag="PLW13_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HQY69672.1"
                     /translation="MRTMTKKAALLSAALAVTLLSGCACNDRLDALETRVGTVEKTAN
                     DALAAANRAQSTADGAAAAAADAQKSANDAVEAVSRMAEKCCRK"
     gene            complement(5620..5706)
                     /locus_tag="PLW13_04560"
     tRNA            complement(5620..5706)
                     /locus_tag="PLW13_04560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5670..5672),aa:Leu,seq:caa)
     gene            5855..6940
                     /gene="queA"
                     /locus_tag="PLW13_04565"
     CDS             5855..6940
                     /gene="queA"
                     /locus_tag="PLW13_04565"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007948187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQY69673.1"
                     /translation="MKVCDFRYELPDDLIARHPCAERTGARLLWLDPAREHPEHLQFA
                     DITRLLRAGDLLVFNDTRVIPARMFGCKAATGGAVEVLVERLLGTHRVLAQLRASKAP
                     KPGSELLLLDEEGREAARVRVLGRHDDMFELEFPADRAPLAVMQSIGHMPLPPYIDRE
                     DGALDRERYQTVYAARDGAVAAPTAGLHFDAALIEALRAIGVQTAFLTLHVGAGTFQP
                     VRTAQVEDHRMHAEYIDVGEELCASVMRAHAEGRRVIAVGTTSLRGLEAASDETAIRP
                     FQGDTSIFIYPGYRFRCVDALVTNFHLPESTLLMLVSAFAGRERVLAAYAEAVERRYR
                     FFSYGDAMFVQGARPPLLKSAPGGRNG"
     gene            6948..8063
                     /gene="tgt"
                     /locus_tag="PLW13_04570"
     CDS             6948..8063
                     /gene="tgt"
                     /locus_tag="PLW13_04570"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQY69674.1"
                     /translation="MQFSLLARDGAARRGRLQFPRGEVETPAFMPVGTYGTVKGMLPR
                     DLEEIGAQIVLGNTFHLMLRPGSGIIRDLGDLHAFMGWKRPILTDSGGFQVFSLGDMR
                     KISEDGVRFRSPVDGSAVFLDPETSMRVQRELGSDVVMVFDECTPYPASLEQARDSME
                     LSLRWAWRSKAAHAGNPAALFGIVQGGMYPELRSRSLEGLLAIGFDGYAIGGLSVGES
                     KDEMLMVLDHLGPRLPVERPRYLMGVGTPLDILAAVARGVDMFDCVMPTRNARNAYLF
                     TSRGVLKIRNAVNRTSEEPIDADCGCYTCRNFSRAYLHHLDRCGEMLSGQLNTIHNLY
                     FYQQLMRGIREAIAEQRFADFSAAFVARYAEGRRGAT"
     gene            8060..8380
                     /locus_tag="PLW13_04575"
     CDS             8060..8380
                     /locus_tag="PLW13_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HQY69675.1"
                     /translation="MSSYQVFVYVGPAHAEALKEAMFAAGAGRLGNYSGCAWQTLGTG
                     QFRPGDAARPFIGSPGQDERVEEWRIEMPCAGEHLEAVLAALRAAHPYEEPVCGVLRL
                     EHGG"
     gene            8382..8945
                     /locus_tag="PLW13_04580"
     CDS             8382..8945
                     /locus_tag="PLW13_04580"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQY69676.1"
                     /translation="MLSRIRERLLCEPQEAPGDADAAVLIAVTAEREPQVILTVRASH
                     LRSHPGEVAFPGGRRDAADRSLIETALRESHEEIGLDAGCVEILAAGRQRTSRFGVRV
                     YPFVGVVPADVQLSANPAELEEVFRVPLAYFLQRENLRVQRIVDAGRELDVAWYPWRE
                     RQVWGMTAVMLVDLLNTVFDFGVELRR"
     gene            8966..9493
                     /locus_tag="PLW13_04585"
     CDS             8966..9493
                     /locus_tag="PLW13_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQY69677.1"
                     /translation="MIYELDGRRPSIDASCFVAPNASVIGSVTLGAEASVWFNVVIRA
                     DHESIAIGAGSNIQDGSVLHADPGYPLLIGERVTVGHQAMLHGCTVGDGSLVGIGAVV
                     LNGAVIGRNCLIAANSLVTEKMQVPDGALVMGSPGRVQRILDEHQRATIARNALHYIA
                     NARLFRERLQELGND"
     gene            9486..9683
                     /locus_tag="PLW13_04590"
     CDS             9486..9683
                     /locus_tag="PLW13_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQY69678.1"
                     /translation="MTDPRADVKSPCIGVCALDARDVCIGCYRSAAEIGAWTTLTNED
                     KRAVLERADRRYREEWGQAAR"
     gene            complement(9687..10946)
                     /locus_tag="PLW13_04595"
     CDS             complement(9687..10946)
                     /locus_tag="PLW13_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HQY69679.1"
                     /translation="MNDSSLELLLGILIACLLMSAFFSASETGMMSLNRYRLKHLRKN
                     HRGARLATRLLERPDRLLGLILIGNNLVNILASAIATVIALRVWGDAGIAIATGAMTV
                     LVLIFGEVTPKTLAALHPERIAFPAAYVLNALMYVFYPLVWCINQITNGLLKVLGINP
                     DKRPEDALSSEELRTIVGESGPMIPPRHKRMLLNILDLEEVTVDDIMIPRHEIRGIDV
                     EEDDAQIIEALRTSEHTRLILYREDIGNILGILHLRTVSLFLNGDRLDREAMQRTAAD
                     PYFVPEGTPLNTQLLNFQKLKRRMGIVVDEYGSVRGLVTLEDILAEIVGEFTSNISDQ
                     SPEIVPQPDGSFVIDGATAIRDINRTLHWELPIDGPKTLNGLILEFLETIPEAHVGMR
                     LGRYHFEILELQGKVIHKVRASRPRGG"
     gene            complement(11031..11843)
                     /gene="ccsA"
                     /locus_tag="PLW13_04600"
     CDS             complement(11031..11843)
                     /gene="ccsA"
                     /locus_tag="PLW13_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQY69680.1"
                     /translation="MTTVLSGLAACLFYLASLASHLFALHEGRAQQRSLRLAMLLALP
                     ALLLHLLSWLAEAVTAQGLDFGLFNAASLLFWVVAALAWGASWRLPLANLLLVVYPLT
                     VVAVLCALFLHSPYSPRHDLGWGMGAHILLSVLAYSILGIAAVQAVALGLLINRLKHR
                     RLHGVVDVMPPLQTMEYLLFRLIWTGEVLLALAILTGALFLENMFAQHLAHKTVLSIV
                     AWLVFAVLLWGKHRLGWRGTTAVKWTLTGFGLLILAYFGSKFVLELLLQRPA"
     gene            12020..13387
                     /gene="ffh"
                     /locus_tag="PLW13_04605"
     CDS             12020..13387
                     /gene="ffh"
                     /locus_tag="PLW13_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQY69681.1"
                     /translation="MFDTLSERLSETLRRVAGKATLSEDNIKDTLREVRQALLEADVA
                     LPVVKDFIDQVRERAVGQEVMKSLSPGQAFLRIVQLELERAMGTANETLDLATQPPAV
                     ILMAGLQGSGKTTSAAKLARWLKEKGKKVLMVSADVYRPAAIRQLETLAESVGVGFVP
                     SHEGQRPLDIAQGALQTARIQFADVLIVDTAGRLAIDAAMMQEIRDLHAAVKPVETLF
                     VVDAMTGQDAAQTARAFNEALPLTGVILSKADADSRGGAALSVRAITGKPIKFLGVGE
                     KTDALEPFYPDRIASRILGMGDILSLIEEAERKLDRDKAEKLARKIKKGKGFDLEDFR
                     DQLQQMRDMGGISSMLDKLPGMGGLAQMAQQKVDTKMFVRMEAIINSMTPKERGDPDM
                     LNGSRKRRITEGSGTTIQDLNRLLKQHKQMQKMMKKLGNKSAMQNMMRGLGGMMPPGG
                     GFPRR"
     gene            13536..13796
                     /gene="rpsP"
                     /locus_tag="PLW13_04610"
     CDS             13536..13796
                     /gene="rpsP"
                     /locus_tag="PLW13_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQY69682.1"
                     /translation="MVTIRLSRVGAKKRPFYQVTVTDSRSARDGRFIERVGFFNPIAS
                     GKEERLRLDHGRIDFWVKQGAQLSDRVAQLVQESARKGAQAA"
     gene            13806..14351
                     /gene="rimM"
                     /locus_tag="PLW13_04615"
     CDS             13806..14351
                     /gene="rimM"
                     /locus_tag="PLW13_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616348.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQY69683.1"
                     /translation="MNTPADSDLVVLGRISGVYGIKGWLKVWSFTDPLEGILDYPAWH
                     LKTATGWDVREIDAGRRQGKGIVVHLPGCDDRDQAQQFLQAEIAVPRAALPELDDGEF
                     YWNELEGLQVRARLADGTTVLLGRLDHLMETGANDVLVVRACDGSLDQRERLIPWIPD
                     EVILAVDRARRELLVDWDPEF"
     gene            14371..15063
                     /gene="trmD"
                     /locus_tag="PLW13_04620"
     CDS             14371..15063
                     /gene="trmD"
                     /locus_tag="PLW13_04620"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQY69684.1"
                     /translation="MLRVAVVTIFPEMFDALTGHGISGRACREGLVELRCWNPRDYAS
                     DRHRSVDDRPYGGGPGMLMMAPPLCAAIRDAKRSLGEGARVIYLSPQGAPLTQRRVEE
                     LAAGGPLALVAGRYEGVDERVIESEVDEELSLGDYVLSGGELPAMVLLDAMIRLLPGA
                     LGHGESAAQDSFAEGLLDCPHYTRPEVFEGRGVPPVLLSGNHERIRAWRHEQATARTR
                     SRRPDLLAKGED"
     gene            15145..15513
                     /gene="rplS"
                     /locus_tag="PLW13_04625"
     CDS             15145..15513
                     /gene="rplS"
                     /locus_tag="PLW13_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQY69685.1"
                     /translation="MQNTIIGQLEAEQMGKNVPDFSPGDTVVVDVRVVEGDRERLQAY
                     EGVVIGKRNRGLNSAFTVRKISHGVGVERTFQTYSPTVANIQVKRRGDVRQAKLYYLR
                     ELSGKAARIKEKLTSKASAE"
     gene            15572..16471
                     /gene="xerD"
                     /locus_tag="PLW13_04630"
     CDS             15572..16471
                     /gene="xerD"
                     /locus_tag="PLW13_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQY69686.1"
                     /translation="MPAHAADAAIEQFLDALWMEKGLSANTLLAYRHDLEAFAGWLAP
                     RGLALDAADRAAILDYLADGLRAGKSARSAARQLSCLRNFYRHLLREGRIAADPTLQV
                     QSPRIGRALPRSLGEDDVDSLLAAPDTATAIGLRDRAMLELLYACGLRVSELVTLELG
                     RVNLGQGVVRIVGKGARERLVPVGDEALAWLARYLREARGQLVPQGSTTMLFPGRGGA
                     ALTRQAFWHRIKQHAAAAGITKPLSPHTLRHAFATHLLNHGADLRVVQMLLGHSDLST
                     TQIYTHVARERLKQLHAAHHPRG"
     gene            16541..17284
                     /locus_tag="PLW13_04635"
     CDS             16541..17284
                     /locus_tag="PLW13_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HQY69687.1"
                     /translation="MVFAKLQRLIGGVLLGGMIMGAGARAEDPQAEVILKALSAARPD
                     LQYEQPRPSPVAGLYEVQVKGGPLLYIDADGSHFIAGDLFAVQKSGFVNLAEQGRQKE
                     RAELIAAVKPEDMIIFAPENPKATIAVFTDVDCGYCRKLHSEVAELNALGIAVHYLAF
                     PRAGLGSPSFRKIASAWCAKDPGAALTSLKNGEEIPENVCADNPVAAQMLLGEQVGVN
                     GTPALVLEDGTLVPGYRPAKDLAKLLGIE"
     gene            17439..18746
                     /locus_tag="PLW13_04640"
     CDS             17439..18746
                     /locus_tag="PLW13_04640"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQY69688.1"
                     /translation="MDPIRVGICGFGTVGSGTFSVLARNSQDIAARVGRDVIVTHIGA
                     RRDNPRCDTGATRLSRDVFAVVRDPEVDIVVELIGGCDTARELVMEAIAAGKHVVTAN
                     KALIAQHGNQIFAAASARGVTVAFEAAVGGGIPIIKAIREGLAANRIEWVAGIINGTG
                     NYILSEMREKGRSFADALAEAQALGYAEADPTFDVEGIDAAHKLVILASLAFGIPLQF
                     EKVYTEGIGALAREDLAYAAELGYRIKHLGICRRTPAGIELRVHPTLIPYDRLIANVN
                     GVMNAILVKGDAVGPTLYYGPGAGSEPTASAVVADIVDVARTLTADPNHRVPHLAFQA
                     DRLSSEPVLPMSAVQTSYYLRVAAVDRPGVLWRVAQILSEAGISIEALIQKEAPEGSE
                     TATMILLTSVTIESRMSDAIAAIEALESTRGRVTRIRMEKLDG"
     gene            18771..20165
                     /gene="thrC"
                     /locus_tag="PLW13_04645"
     CDS             18771..20165
                     /gene="thrC"
                     /locus_tag="PLW13_04645"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQY69689.1"
                     /translation="MRYISTRGQAPALAFEDVMLTGLAPDGGLYVPETLPRFSAGQMR
                     AWAKLDYADLAATIMQPFVEGFLAEDELRAITRETYAGFRHPAVAPLVQLGHNEWLLE
                     LFHGPTLAFKDFALQLLGRLLDVSLARRHQRVVVLGATSGDTGSAAIEGCRRCANVDI
                     FILHPHQRVSEVQRRQMTTVPGDNIHNIAVQGNFDDCQEMVKQSFSDQSFLPEGRRLV
                     AVNSINWARIMAQIVYYFHAALALGAPERAVSFAVPTGNFGDIYAGYLARRMGLPVRQ
                     LVIATNANDILHRFMSSSRYGKSALAHTLSPSMDIVISSNFERALFEFYGRDAAALAD
                     LMQRFRSSEVAVPESVIAAARAEFASFSVDDAMTCATIAGVYEESEYLLDPHSAIGVR
                     AARECRDDPGVPMVTLATAHPAKFPEAIARAGVAREPALPHYMADLFERPERCAVLPH
                     EVRAVQEFIRGRIA"
     gene            20173..>21803
                     /gene="recJ"
                     /locus_tag="PLW13_04650"
     CDS             20173..>21803
                     /gene="recJ"
                     /locus_tag="PLW13_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQY69690.1"
                     /translation="MRRAIRRRSARADAASAPGVSPLLARLYAARGVADASDLEQGLA
                     QLLPPVLLGLEQAATRLADALARDERILIVGDYDADGATSTALGLLALRAMGCTSADF
                     LVPNRFEHGYGLGPEIVAVAAALHPRLIITVDNGIASHAGVAAAAAHGIDVLVTDHHL
                     PGETLPAACAIVNPNQPGCAFPSKSLAGVGVVFYLMGALRARLRASGWFAARGLAEPN
                     LGELLDLVALGTVADVVALDANNRILVRQGLQRIRAGRGRPGVRALLELAGRNPARVV
                     SADLGFAVGPRINAAGRLDDIGLGIRCLLEEEPARAREMAAELDAWNRERRDIEGGMQ
                     QEALRLMEQGAIAEDALPWGPCLYHPDWHQGVVGLVASRIKEKHHRPVIAFAPGGAGE
                     LKGSARSIRGFHIRDAIAAVAAAAPGLVERFGGHAMAAGLTLAAERFEEFARAFDAEV
                     RRHLEASDLESVLESDGELGAGEFSLAQAEELREAGPWGQHFPEPLFDGEFWLRSQRL
                     LGGKHLKLVVSPVAAPQLELEAIAFNIDAALWPDAR"
BASE COUNT         3529 a         7148 c         7747 g         3379 t
ORIGIN      
        1 gacgacgatg ctggccggca tgggcgagca tctgctgtcg gagtattaca tcgtgcgcgc
       61 tccggcgctg cgcgagatgg aactggacga agaggaaatg acggagtgga tctcggtcat
      121 gttctatcgc gccctgttcc tcgagaaccc gcctgcggag cggttgaagc atgccgcact
      181 ggtaacacac ctgcggcgca gcgccggggt gccgggcagg atttgaagca aacaacccca
      241 taccgggagg atgcattgtc gtgagcgaga cagacgagac gattcagagc tacgtgggcc
      301 gcccgccctg cggtccctac aacagctggg acgaggtgaa cgcgccgatg atccggcagc
      361 tgtgcgaggc gctgggcgac gagaacccgg tctacatcga cccggcgttc gcggcgcagt
      421 cggtgcacgg cgggctcgtg gcgccgccga cgatgctgca ggtctggacc atgcgcggcg
      481 tcggtggcaa gtgggctccc ggctcggaca gccgcgagcc cttcgaggtg ttcgagtacc
      541 tgaagacgat cggcttcccg gccgtggttg cggtgaactg cgagcagaac tactaccgct
      601 acgtgaagcc gggcgacaag ctgcaccaca cctcgagcat cgagtcgatc tcgccgcaga
      661 agaccactgc gctcggcacc ggctatttcg tgaccgaact cgaggagttc ttcgaccaga
      721 acggcgagaa ggtcggcgac atgcgttttc gcgtgttcct ctaccgcgcg ccggaccgtg
      781 cgcaggaagc ggctcccgcg ggcgaagccg cgaagccggc gaagatcaag cgttttcgcc
      841 cgatcgagaa ccacgacacg aagttcttct gggaaggcgt gcgccagggc cagttgctga
      901 tacagcgctg caaggcctgc aacacgctgc gccacccgcc ggggccgatg tgtccctcgt
      961 gccagtcgct cgagtgggac accgtgagca gttccggcaa gggcacgatc tacagcttcg
     1021 tcgtgatgca ccatccgcag atcccgccgt tcgattaccc gaatacgatc ctgctggtcg
     1081 agctcgagga aggcacgcgc ctggtgagcc aggtcatcga cacgcacccg gacgaggtgc
     1141 agatcggcgc cgcggtcgag gtggtgttcc gcgaagtcga ggaaggctgc acgctgccgc
     1201 tgttccgcgt cgtgaaataa ataagggagg tcgaacatgg atttcacgcg ttccgaagaa
     1261 tcgcaatcga tccaggacct ggcggcacag atcttcgccg accaggccgg cgacgaggcg
     1321 ctcaagcgct tcgaccgcca cgagatcgcc taccacaccg gcttgtggaa attgctcgcc
     1381 gaggcgggcc tgatcggggt cgcgctggcc gaggaacacg gcggcagtgg actgggcttc
     1441 accgagctgt gcgcggtgtt cgaggagcag ggccgccacg tcgccgcgct gccgctgctg
     1501 gccaccgtgg tgctgggcgc gctgccgatc gcggcattcg gcagcgaggc acagcgcaca
     1561 cagtggctcc cgggcgtggc gcgcggcgaa ctgctgctga gcggcgccct gtgctgtccg
     1621 gaggtcggag gacatgccga ccggctgccg cgcgcgagcg cggacggtgc gggctggcgt
     1681 ctggacggca cgctggaccg ggtgccatac agcctggaag ccgcggcgat cgtggtgccg
     1741 gcgcaaggcg atgacggcgc gatgcacgtg ttcctggtgc cggccaaagc cgcgggcctg
     1801 gagcgcgagc gccagcgcgg caccaaccat gaaccggtcg atacgctggc attcacgggc
     1861 gtgaaagtgg cgtccgcgga cctgctcggc aagccgggcg agggcgcctg catcgccgag
     1921 tggatcggca cgcgcgccgc gacggcgctc ggcttcatgc agctcggcgt gctgtcggac
     1981 gcgctgcgcc gcaccgccgc gcacacggtc gagcgcaagc agttcggcaa gccggtcgcg
     2041 ggcttccagg ccgtagcgca ccgcgcagcc gacgcctaca tcgacgtcga ggccctgcga
     2101 tccacggcgt ggcaggcagc gtggcgcatg tccgagagcc ttcctgcgga agccgcggtg
     2161 gcttcggtcg cgtggtgggc ctgcgaggcg gggcaccggg tcggtcacgc ctgccagcac
     2221 ctgcacggcg gcgccggcgc cgacctcgac gcgccgatcc accgttactt cctgtgggcg
     2281 cggcaggtcg agctctgcct cgggggcagc aaccggatcc tggcccgctt cggcgccagc
     2341 ctttccaacc agaccatcga gatcggacta tgagcattca ggacaggacc accctcaagt
     2401 tcgcggatgt gcgggtcggt gacgagttgc cgccgctcgg catcgagctc accacgcgcc
     2461 tgatcgtctc gacggcactg gccacgcgtg atttccagga cgtgcaccac gacaagccgg
     2521 gtgcgcaggc gctgggctcg ccggacatct tcatgaacat catcaccacc aacggattcg
     2581 tcggccgtta cgtcaccgac tgggccggtc cggaggcgct gttgcgctcg gtgaagatcc
     2641 gcctcggcgc gccgaactat cccggcgaca cgatggtgat caagggcaag gtgaccgagg
     2701 tgcacagcga cgcgaagacc gtcgacatcg aggtcggcgg caagaatggt ctgggctacc
     2761 acgtcaccgg cacggtgacc gtggcgctgc cctgaggaga acacgatgag cgacaacaag
     2821 tttctgagcg gcaggacggc cgtcgtcggc ctgggtgcca ccgagttttc gaaggattcc
     2881 gggcgcagcg agatccagct cggcgtcgag gcgatcagca tggcgctggc cgacgcgggc
     2941 ctgtcgatcg acgaagtcga cggcatggcc acctacacca tggacaacaa ccccgagatc
     3001 gagctctacc ggctcctcgg cggcaaggag ctgaaattct tctcgcgcac gcattacggt
     3061 ggcggcgcgg cctgcggccc gctgctgcac gcggcgatgg cggtggccac cggcgtgtgc
     3121 aaggcggtcg tggtctaccg cgcgatgaac gagcgctcgg gcttccgctt cggccagggt
     3181 gggctgatca gcaacgaccc gaattctttc gagaccgtgc acttcggctg gtatatgccg
     3241 ttcggcctgt cgaccccggc gtcgtgggtc gcgatgtgtg ccaagcgcta cctgcacacc
     3301 cacggagcgg agccggacga tttcggccgt gtggcggtat cgacgcgcga tttcgccgcg
     3361 accaacccgg cggcgttctt ctaccagaag ccgatcacgc tcgacgatta ccgcaaatcg
     3421 cgctggatcg ccgagccgct gcgcctcttc gactgttgcc aggagagcga cggcgcggtg
     3481 gccatggtga tcacctcgat cgagcgcgcg cgtgacctga agcagaagcc cgtggtcatc
     3541 cgcagcgcgg cgcagggcgc ggccggcgat ttccagatga tgaccggctt ctaccgtcca
     3601 gacctgacgg cgatcccgga gatgggcctg gtcgcgcgcc agctctacgg catggccggc
     3661 gtgggaccgc aggatatcca gaccgcggtg ctgtacgacc acttcacgcc gttcgtgttg
     3721 ccgcagctcg aggagttcgg cttctgcggg cgtggcgagg ccaaggactt cattcgcgcc
     3781 ggtcacatct cgcgcggcgg caagctgccg gtaaacaccc acggcggaca gattggcgag
     3841 gcctacatcc acggcctgaa cggcgtggcc gagggcgtgc gccaggtgcg tggcacggcg
     3901 gtcaaccaga cgccaggcgt ggagcatgcg ctggtcaccg cgggcaccgg tgtgccgacc
     3961 agcggggtga ttctcggcgt cgaccgatag ccgggacggc gtgacattgg ccggtcgggt
     4021 cgagacgccc cgccttctgt tgctagaatg cggggccaat tccctttccg ggcggtacag
     4081 aaacagatgc gcaagctgtt actgcttttc ggtgttctct gctcgctgca cgcgacagcc
     4141 ctgacgctgg acatgccttc tcccggcgag gacatcgtcg gcgaggtcat gacggtcacg
     4201 gtggccgact acgagcacac catcaatacc tacgcggaag cctacggtgt cggctttcgt
     4261 cagctcgttg ccgccaaccc cggtgtcaac ccgtgggtgc cgggcgaggg caccgaactg
     4321 gtgctgccgc tgcagttcat cctgccgccg ggcgaacgcg agggcatcgt gatcaacctg
     4381 gctgaaatgc gcatgtacca ctaccagccc gatggcggca cggtggagac ctacccgatc
     4441 ggcatcggcc gtgaaggctg ggaaacgccg ctggttgcgg gcgcgaaggt gctcagtgtg
     4501 atcaaggacc cgacctgggt gccgccgccc tcgatccgcg cggatcacct ggccatgggg
     4561 caccgtctgc cggcggtggt gccgccgggt ccggacaacc cgatgggtcc gtgggcggtg
     4621 cagttgagca ccaggggcta ctacatccac ggcaccaaca aggagctcgg catcggcatg
     4681 cgcgtcagtg ccgggtgcat ccgcatgtac aacaaggacg tggagcagtt tgcccgtcgc
     4741 gtagccaagg gcacgacggt gcgcatcgtc aacgcgccgg tgaaggtggg ctggaagcag
     4801 ggggagctct acgtcgaggt gcacgaggcg ctcgaggagc agcgcgaggt ccacgtgccg
     4861 gaagccgacg tggccgatgc catccacctg gcgcgccgcc tgagctcgac gccggtggaa
     4921 atcaactggg tcggtgccaa ggctgctgcc ctgcgccgtt cgggcatgcc ggtccgcgtt
     4981 tccgtggacg attccgccgg tatcgccagc tcgcgctgac ggcctcccgt gcgctggaac
     5041 acgtgctcag cccacggatg caaatttcac gtatccccga aatgagactg ccccgcggac
     5101 accgccgtga aggcggtgcg cgcggggcag cgacgcaaga tcgggaccgg gtctgtggac
     5161 ccggctcaga tcacttgcgg cagcacttct cagccatgcg gctgacggct tcaactgcgt
     5221 cgtttgcgct cttctgagcg tcggcagcag ctgcagcagc gccgtcggcg gtcgattgtg
     5281 cgcggttggc agcggccagc gcgtcgtttg cggtcttctc aacggtgcct acgcgggtct
     5341 cgagcgcgtc gaggcgatcg ttgcaggcgc aaccgctgag cagcgtaacg gcgagtgctg
     5401 cggacagaag ggcggctttc ttggtcatgg tacgcatctg tggtctcctt gaaggtcaat
     5461 catgcgatcg ggtcgatagc ggtgaaattc ttgcacatct gacttcgaag tcaatgttag
     5521 ttctgcataa aattgacgaa gcgcaggttt gccggcgctt tgtgacggcg ttgttgcgga
     5581 cacgcctcga ccggcgtgcc gaatcaaccg ataagcgggt ggtaccagag gcgggactcg
     5641 aacccgcacg ccctcgcgga caacggattt tgaatccgtc tcgtctacca gttccgacac
     5701 tctggcccag tgaagcgcgc ggcattatag caatgcatct ttgcaccgca accgcaaccg
     5761 caaccgcaac cgcaaccgca accgcaaccg caaccgcaat tgccttggcg ccggtgcctc
     5821 tatacccttg ccgccttgcc ggcacgggtc tgccatgaaa gtctgcgatt tccgctatga
     5881 actgcccgat gatctcatcg cgcgccatcc ctgcgccgag cgcaccggcg ctcgcctgct
     5941 gtggctcgat cccgcgcgcg agcatcccga gcacctgcag ttcgccgata tcacgcgcct
     6001 gctgcgcgcg ggcgacctgc tggtgttcaa cgacacgcgc gtgattcccg cgcgcatgtt
     6061 cggctgcaag gccgccaccg gtggcgccgt ggaggtgctg gtggagcggc tgctgggcac
     6121 tcatcgcgtg ctggcgcaac tgcgcgccag caaggcgccg aagcccggca gcgaactgct
     6181 gctgctcgat gaagagggcc gcgaggccgc gcgcgtgcgt gtgctcggcc ggcacgatga
     6241 catgttcgag ctggagtttc ccgcggatcg cgccccgctc gcggtgatgc agtccatcgg
     6301 gcacatgccg ctgcctccct acatcgaccg ggaggacggc gcgctcgatc gcgagcgcta
     6361 ccagacggta tacgcggccc gcgacggcgc ggtggcggcg ccgaccgccg ggctgcattt
     6421 cgatgcggct ctgatcgagg cgctgcgcgc gatcggcgtg caaaccgcgt ttctcacgct
     6481 gcacgtgggg gccgggacct tccagccggt gcgcacggcg caagtcgagg atcatcgcat
     6541 gcacgccgag tacatcgacg tgggcgagga actgtgcgcc agcgtcatgc gcgcccatgc
     6601 cgaggggcgg cgcgtgatcg cggtgggcac cacctcgctg cgcggactgg aggcggcctc
     6661 ggacgagacg gcgatccggc cgttccaggg cgatacctcg atcttcattt acccgggcta
     6721 ccgctttcgc tgcgtcgatg cgctggtcac caatttccac ctgcccgagt ccacgctgct
     6781 gatgctcgtc agcgctttcg ctggccgcga gcgcgtgctc gcggcctatg ccgaggcggt
     6841 cgagcgccgt tatcgcttct tcagctacgg cgatgcgatg ttcgtccagg gcgcacggcc
     6901 gccgctgctg aagtccgcac cgggaggccg caatggctga atgcttcctg cagttctcgt
     6961 tgctcgcccg cgacggcgcc gcgcgccgtg ggcggttgca gtttccgcgc ggcgaggtcg
     7021 aaacgccggc gttcatgccg gtgggcacct acggcacggt caagggcatg ctgccgcgcg
     7081 acctcgagga gatcggcgcg cagatcgtgc tcggcaacac cttccacctg atgctgcgcc
     7141 cgggctcggg catcatccgc gatctcggcg acctgcacgc gttcatggga tggaagcgac
     7201 cgatactcac cgattccggc ggtttccagg tgttcagcct cggcgacatg cgcaagatca
     7261 gcgaggacgg cgtgcggttc cgctcgccgg tggacggcag cgcggtgttt ctcgacccgg
     7321 agacctcgat gcgcgtgcag cgcgagctgg gttccgacgt ggtgatggtg ttcgacgagt
     7381 gcacgccgta cccggccagc ctcgagcagg cgcgcgattc gatggagctc agcctgcgct
     7441 gggcctggcg cagcaaggcg gcccatgcgg gcaacccggc cgcgctgttc ggcatcgtgc
     7501 agggtggcat gtatccggag ctgcgcagcc gctcgctcga gggcctgctc gcgatcggct
     7561 tcgatggcta cgcgatcggc gggctttccg tgggcgagag caaggacgag atgctgatgg
     7621 tgctcgatca cctcgggccg cgcctgcccg tggagcgacc gcgctacctg atgggcgtgg
     7681 gcacgccgct cgacatcctc gcggccgtgg cacgcggcgt ggacatgttc gattgcgtga
     7741 tgcccacacg taatgcccgc aacgcctacc tgttcacctc gcgcggcgtg ctgaagatcc
     7801 gcaacgcggt caaccgtacc agcgaagagc cgatcgatgc ggactgcggc tgctacacct
     7861 gccgcaattt cagccgcgcg tacctgcacc acctcgaccg gtgcggcgag atgctgagcg
     7921 ggcagctcaa caccatccac aacctgtact tctaccagca actgatgcgg ggcatacggg
     7981 aggcgatcgc ggagcagcgt ttcgcggact tcagcgccgc gttcgtcgcg cgctacgccg
     8041 agggcaggcg cggcgcgaca tgagcagcta ccaggttttc gtctacgtcg gtcccgcgca
     8101 cgccgaggcg ctgaaggagg cgatgttcgc cgccggagcc gggcgcctcg gcaactacag
     8161 cggttgcgcc tggcagactc tcggtaccgg gcagttccgc ccgggcgacg ccgcgcggcc
     8221 gttcatcggt tcgccggggc aggacgagcg cgtggaggaa tggcgcatcg agatgccgtg
     8281 cgccggcgag cacctggagg cggtgctggc cgcgctgcgc gcggcgcatc cctacgagga
     8341 gccggtgtgc ggggtgctgc gcctggagca cgggggctga gatgctgtcc cggatccgcg
     8401 aacggctgct gtgcgagccg caggaggcgc ccggcgacgc cgatgccgcg gtgctgattg
     8461 ccgtcaccgc cgagcgcgaa ccgcaggtca ttctcacggt gcgcgcgtca cacctgcgat
     8521 cgcacccggg cgaggtcgcc ttcccgggcg gcaggcgcga tgccgcggat cgcagcctga
     8581 tcgagacggc gctgcgcgag agccacgagg agatcgggct ggatgcgggc tgcgtcgaga
     8641 tcctcgccgc cgggcgccag cgcacctcgc gtttcggtgt gcgtgtctat cccttcgtgg
     8701 gagtcgtgcc ggcggacgtg cagctgtcgg ccaatccggc ggagctcgag gaggtgttcc
     8761 gcgtgccgct cgcgtatttc ctgcagcggg aaaacctgcg cgtgcagcgt atcgtcgatg
     8821 cggggcgcga attggacgtg gcatggtacc cgtggcgcga gcgccaggtc tggggcatga
     8881 ccgcggtcat gctggtcgac ctgttgaaca cggttttcga ttttggcgtc gaattgcggc
     8941 gctgatcatt ctccgcgagg tgcacatgat ctatgaactg gacggccgtc ggccgtcgat
     9001 cgacgcgagt tgcttcgtgg cgcccaatgc ctcggtgatc ggctcggtca cgctcggcgc
     9061 cgaggccagc gtgtggttca acgtggtgat ccgcgccgac cacgagtcca tcgccatcgg
     9121 tgccggcagc aacatccagg acggcagcgt gctgcacgcc gatccgggct atccgctgct
     9181 gatcggggag cgcgtgaccg tggggcacca ggcgatgctg cacggctgca ccgtcggtga
     9241 cggcagcctc gtcggcatcg gcgcggtggt gctgaacggc gcggtgatcg ggcgcaattg
     9301 cctgattgcc gccaattcgc tggtcaccga gaagatgcag gtgcccgacg gcgccctcgt
     9361 gatgggctcg ccggggcggg tgcaacgcat cctcgacgag caccagcgcg ccacgatcgc
     9421 gcgcaacgcg ctgcactaca tcgccaacgc gcggctgttc cgcgagcggc tgcaggaact
     9481 gggcaatgac tgacccgcgc gccgacgtga agtcgccgtg catcggcgtc tgcgccctgg
     9541 atgcccgcga tgtctgcatc ggctgttacc gcagcgccgc ggagatcggt gcctggacga
     9601 cgctgacgaa cgaggacaag cgcgccgtgc tcgagcgcgc agatcggcgc taccgcgagg
     9661 agtgggggca ggccgcgcgc tgatgctcag ccgccgcgcg ggcggctggc gcgcaccttg
     9721 tggatcacct tgccctgcag ttcgagaatc tcgaagtggt agcggcccag gcgcatgccg
     9781 acgtgcgcct ccggaatcgt ttccaggaac tcgaggatca ggccgttcaa cgtcttcggg
     9841 ccgtcgatcg gcagctccca gtgcagcgtg cggttgatgt cgcggatcgc ggtggcgcca
     9901 tcgatcacga agctgccgtc gggctgcggc acgatctccg gcgactggtc gctgatgttc
     9961 gaggtgaact cgccgacgat ctccgcgagg atgtcctcga gcgtgaccag cccgcgcacc
    10021 gagccgtact catcgaccac gatgcccatg cgccgcttca gcttctggaa gttcagcagc
    10081 tgcgtgttca gcggcgtgcc ttcgggcacg aaatagggat ccgcggcagt gcgctgcatc
    10141 gcttcgcggt ccaggcggtc gccgttcagg aacagcgaca ccgtgcgcag gtgcagtatg
    10201 ccgaggatgt tgccgatgtc ctcgcgatag aggatcaggc gggtgtgctc gctcgtgcgc
    10261 aatgcctcga tgatctgcgc gtcgtcctcc tcgacgtcga tgccgcggat ctcgtggcgc
    10321 gggatcatga tgtcgtcgac cgtcacttcc tccaggtcga ggatgttcag cagcatgcgc
    10381 ttgtggcgcg gcgggatcat cgggccggac tcgccgacga tggtgcgcag ctcctcggag
    10441 ctgagcgcgt cctcgggacg cttgtcggga ttgattccca gcaccttgag caggccgttg
    10501 gtgatctggt tgatgcacca gaccaggggg tagaacacgt acatcagcgc gttcagcacg
    10561 taggcagcgg ggaaggcgat acgctcgggg tgcagcgcgg ccagcgtttt cggcgtcacc
    10621 tcgccgaaga tcagcaccag gacagtcatc gcgccggtgg cgatcgcgat cccggcatcg
    10681 ccccagaccc gcagcgcgat cacggtggcg atagcggagg cgaggatgtt gacgaggttg
    10741 ttgccgatca ggatcagccc gaggaggcgg tcggggcgct ccagcagccg cgtggcgaga
    10801 cgcgcgccgc gatggttctt gcgcaggtgc ttcaaccggt agcggttgag cgacatcatc
    10861 ccggtttcgg atgcggagaa gaaggccgac atcagaaggc aggcgatgag aatgccgagc
    10921 agcaattcca gcgaggaatc gttcaagcgc aggggtgcct tctgtcagac gggaccggta
    10981 tgttgaagaa aaccgccggg catggcaacc cgggttcgcc ggcggctgcc tcaggccgga
    11041 cgctgcagca gcagttcgag cacgaacttg ctgccgaaat aggcgaggat cagcaggccg
    11101 aagccggtca gcgtccactt gaccgccgtg gtgccgcgcc agccgaggcg gtgcttgccc
    11161 cacagcagca ccgcgaacac cagccacgcg acgatggaca gcacggtctt gtgtgccagg
    11221 tgctgcgcga acatgttctc gaggaacagc gcgccggtga gtatcgccag cgcgagcagc
    11281 acctccccgg tccagatcag ccggaacagc aggtactcca tggtctgcag cggcggcatc
    11341 acgtcgacga cgccgtgcag tcgccggtgc ttgaggcggt tgatcagcag gcccagcgcc
    11401 actgcctgca ccgcggcgat gccgaggatg ctgtaggcga gcaccgagag caggatgtgc
    11461 gcgcccatgc cccagcccag atcgtgccgc ggcgagtagg gcgagtgcag gaacagtgcg
    11521 cacagcaccg cgaccacggt gagcggatac acgaccagca gcaggttggc cagcggcagg
    11581 cgccagctgg cgccccaggc cagcgccgcg acgacccaga acagcagcga ggcggcattg
    11641 aacagcccga aatcgaggcc ctgcgcggtg accgcctcgg ccagccagct cagcaggtgc
    11701 agcagcagtg cgggcagcgc cagcagcatc gccaggcgca gcgagcgctg ctgtgcgcgg
    11761 ccctcgtgca gggcgaacag gtgcgatgcg aggctggcca ggtaaaagag gcaggcggcg
    11821 agtccggaga gaaccgtggt catcgaaagc acgcggcgtc ggcatccagt ggtggaccgg
    11881 gatcagggcc ccggggagcc ggtagtctag cgagcgctgc cgcgggcgtc accccgcgcg
    11941 cttcatgcgc ccgactcgct cgggtatact cgcgcgctat ttttcagcgc cgcccgcggc
    12001 gggacaacgg aactccagca tgttcgacac cctgagtgag cgcctttcag agaccctgcg
    12061 ccgggttgcc ggcaaggcga ccctgagcga ggacaacatc aaggacacgc tgcgcgaggt
    12121 gcgccaggcg ctgctcgagg cggatgtcgc gttgccggtg gtcaaggact tcatcgacca
    12181 ggtgcgcgag cgcgcggtcg ggcaggaggt gatgaagagc ctgagccctg gccaggcctt
    12241 cctgcggatc gtgcagctcg agctcgagcg tgcgatgggc actgccaacg agacgctcga
    12301 cctggccacg cagccgcccg cggtgatcct gatggccggc ctgcagggca gcggcaagac
    12361 caccagcgcg gcgaagctcg cgcgctggct gaaggaaaag ggcaagaagg tgctgatggt
    12421 cagcgccgac gtctaccgcc cggcggcgat ccgccagctc gagacgctgg ccgagagcgt
    12481 cggcgtcggt ttcgtgccga gccacgaggg ccagcgtccg ctggacatcg cgcagggcgc
    12541 gttgcagacc gcgcgcatcc agttcgccga cgtgctgatc gtcgacaccg ccggccggct
    12601 cgcgatcgac gcggcgatga tgcaggagat ccgcgacctg cacgcggccg tcaagccggt
    12661 cgagacgctg ttcgtggtcg acgcgatgac cggccaggac gcggcgcaga ccgcgcgcgc
    12721 cttcaacgag gcgctgccac tgaccggcgt gatcctgtcc aaggcggacg ccgattcgcg
    12781 cggcggcgcg gcgctgtcgg tgcgcgcgat caccggcaag ccgatcaagt tcctcggcgt
    12841 tggcgagaag accgatgcgc tggaaccctt ctaccccgac cggatcgcct cgcgcatcct
    12901 cggcatgggc gacatcctgt cgctgatcga ggaggccgag cgcaagctcg accgcgacaa
    12961 ggccgagaag ctcgccagga aaataaagaa aggcaagggc ttcgatctcg aggattttcg
    13021 cgaccagctg cagcagatgc gcgacatggg cggcatcagc agcatgctcg acaagctgcc
    13081 gggcatgggc gggctggcgc agatggcgca gcagaaggtc gatacgaaga tgttcgtgcg
    13141 catggaggcg atcatcaatt cgatgacgcc gaaggagcgc ggtgacccgg acatgctgaa
    13201 cggatcgcgc aagcgccgca tcaccgaagg ctccggcacc acgatccagg acctcaaccg
    13261 cctgttgaag cagcacaagc agatgcagaa gatgatgaag aagctcggca acaagagcgc
    13321 gatgcagaac atgatgcgcg ggctcggggg catgatgcca cccggcgggg gattcccgcg
    13381 gcggtaaaaa tccgcatacc tcggggcggg tgaccgcaac tgtttcaaat acaagtgctt
    13441 tctcttagaa tgctcgccct ttgcggggcc ggcggcgtgt ggcggctggc gtcggcaacg
    13501 gcgaatcccc tgggatcaac ggcaggaacc ttgtcatggt aacgatcaga ctttcacgtg
    13561 taggcgccaa gaagcgtccg ttctatcagg tcacggtgac cgacagccgc agcgcacgcg
    13621 acggccgctt catcgagcgc gttgggttct tcaacccgat cgccagcggc aaggaagagc
    13681 gcctgcgtct cgaccatggc cgcatcgatt tctgggtgaa gcagggcgcg cagttgtccg
    13741 accgcgtggc gcagctggtg caggaaagcg ccaggaaggg cgcgcaggcg gcctgacagg
    13801 ctgtcgtgaa cactcccgcc gacagcgatc tggtggtgct gggtcgcatc agcggggtgt
    13861 atggcatcaa gggttggctg aaggtctggt ccttcaccga cccgctcgag ggaatactcg
    13921 attacccggc gtggcacctg aagaccgcga cgggctggga cgtccgcgag atcgacgccg
    13981 ggcgccgcca gggcaagggc atcgtggtgc acctgccggg ctgcgacgac cgcgaccagg
    14041 cgcagcagtt cctgcaggcc gagatcgcgg tgccgcgggc ggcactgccg gaactggacg
    14101 acggcgagtt ttactggaac gagctcgagg ggttgcaggt gcgggcgcgg ctggcagacg
    14161 gcaccacggt gctgctgggt cgcctcgacc acctgatgga gaccggggcc aatgacgtgc
    14221 tggtggtgcg tgcctgcgac ggcagcctcg atcagcgcga gcggctgatt ccctggatcc
    14281 ctgacgaggt gatcctggcg gtggaccgcg cgcggcgcga gctgctggtc gactgggacc
    14341 cggagttttg acccgcgagg cacgacagtc atgttgcgcg tcgcggtggt gacgattttt
    14401 ccggagatgt tcgatgccct gaccgggcac ggcatctcgg ggcgggcctg ccgcgagggg
    14461 ctcgtggagc tgcgctgctg gaacccgcgc gactacgcga gcgaccggca caggtcggtc
    14521 gatgaccggc cctacggcgg cgggcccggc atgctgatga tggcgccgcc gctgtgcgcg
    14581 gcgatccgcg acgcgaagcg ctcgctgggc gagggtgcgc gggtgatcta cctgtcgccg
    14641 cagggcgcgc cgctgacgca gcggcgcgtc gaggaactgg cggctggcgg gccgctcgcg
    14701 ctggttgcgg gccgctacga gggcgtcgac gagcgcgtga tcgagagcga ggtcgacgag
    14761 gaactgtcac tcggcgacta cgtgctgagc ggtggcgagc tgccggcgat ggtgctgctc
    14821 gatgcgatga tccggctgtt gccgggtgcg ctcgggcacg gcgagtcggc ggcgcaggat
    14881 tcgtttgcag aggggctgct cgactgcccc cactacacgc ggcccgaggt cttcgagggg
    14941 cgcggggtgc cgccggtgct gctgagcggc aaccacgaac gcatcagggc ctggcgccac
    15001 gagcaggcta cggcacgaac ccggtcgcgc aggccggatc tgctggcgaa gggtgaggat
    15061 tgaggggcag cgccccgggg gcgttgcgca acgggcaagg ggctgcgcca gagtgcgcgg
    15121 tttggatcaa ctgaggaaca ggtcatgcag aacactatca ttggacagct cgaagccgag
    15181 caaatgggca agaacgtgcc ggatttctcg ccgggcgaca cggtggtcgt ggacgtgcgc
    15241 gtggtcgaag gcgaccgcga gcgtctgcag gcctacgaag gcgtcgtgat cggcaagcgc
    15301 aaccggggcc tgaactcggc gttcaccgtg cgcaagatct cgcacggcgt gggcgtggag
    15361 cgtacgttcc agacctacag cccgacggtg gccaacatcc aggtcaagcg tcgcggcgac
    15421 gtgcgccagg cgaagctgta ctacctgcgc gaactgagcg gcaaggcagc gcgcatcaag
    15481 gaaaagctca cgtccaaggc cagcgcggaa taagcggcac tgtcgctggc ggaaaaggtg
    15541 gcgcaagcca ccttttttgt tgcaccttcc gatgcccgca catgccgccg acgcagcgat
    15601 cgagcaattc ctcgatgcgc tgtggatgga gaagggcctg agcgcgaaca cgctgctcgc
    15661 gtatcgccac gatctcgagg ccttcgcggg ttggctggcg ccgcgcgggc tggcgctgga
    15721 cgcagcggat cgcgccgcga tcctcgacta cctggccgac ggcctgcgtg ccggcaagtc
    15781 ggcgcgcagc gcggcgcgcc agctgtcgtg cctgcgcaat ttctatcgtc acctgctgcg
    15841 cgaggggcga attgccgcgg acccgacgct gcaggtgcag agcccgcgca tcggccgtgc
    15901 gctgccgcgc agcctcggcg aggacgacgt cgacagcctg ctggcggcgc ccgataccgc
    15961 caccgcgatc ggcttgcgcg accgggcgat gctcgagctg ctgtatgcct gcggactgcg
    16021 cgtcagtgaa ctggtgacgc tggagctggg gcgggtcaat ctcgggcagg gcgtggtgcg
    16081 catcgtgggc aagggcgcgc gcgagcggct ggtgccggtc ggggacgagg cgcttgcgtg
    16141 gctcgcgcgc tacctgcgcg aggcacgggg acaactggtg ccgcagggca gcaccacgat
    16201 gctgttcccg ggccgtggtg gcgcagcgct gacccgccag gccttctggc accggatcaa
    16261 gcagcacgcc gcggcggcgg gcatcacgaa gccgttgtcg ccgcacacgc tgcgccatgc
    16321 cttcgcgacg cacctgctga accacggcgc ggacctgcgc gtggtgcaga tgctgctcgg
    16381 gcacagcgat ctctcgacca cgcagatcta cacgcacgtg gcgcgcgagc gcctgaagca
    16441 gttgcacgcg gcgcaccatc cgcgcggatg aggcgttgaa ccaaaaacca gcggcggttt
    16501 ccaacccgca gcaatcaacg gcaaagaggg agttcacgtg atggtattcg cgaagttgca
    16561 gcgcctcatc ggcggtgtcc tgctgggtgg catgatcatg ggtgccggcg cccgcgcgga
    16621 ggatccgcag gccgaggtca tcctgaaggc gctcagcgcc gcgcgcccgg acctgcagta
    16681 cgagcagccg cgcccgagcc ccgtggcggg cctctacgag gtgcaggtca agggcggccc
    16741 gctgctctac atcgacgccg acggcagtca cttcatcgcc ggcgacctgt tcgcggtgca
    16801 gaaatcgggc ttcgtgaacc tggccgagca gggccggcag aaggagcgcg ccgagctcat
    16861 cgccgcggtg aagcccgagg acatgatcat cttcgcgccc gagaatccca aggcgacgat
    16921 cgcggtgttc accgacgtcg actgcggcta ctgccgcaag ctgcacagcg aagtcgccga
    16981 gctgaacgcg ctgggcatcg cggtgcacta cctggcgttt ccgcgcgcgg gcctgggctc
    17041 gccgtccttt cgcaagatcg ccagtgcctg gtgcgccaag gaccccggtg cggcgctcac
    17101 cagtctcaag aacggcgagg agatccccga gaatgtctgc gccgacaacc cggtcgccgc
    17161 gcagatgctg ctcggcgagc aggtcggcgt gaacggcacg ccggcgctgg tgctcgagga
    17221 cggcacgctg gtgcccggtt accgtccggc gaaggatctg gcgaagctgc tcggtatcga
    17281 gtgagcgcag cggcgcgcgc gggtgcgccc ggcgcggtga ttcgggatgc aagtcctgct
    17341 atgatcgcgc gcctcgtaat ccgtgcccgg ccgggcgcag cacccgctgc gcccgccacg
    17401 ccctgcgtgg ccgggccatc atgcaggagc agtttttctt ggatccgatc agggtaggca
    17461 tctgtggttt cggcaccgtc ggctccggga ccttcagcgt gctcgcgcgc aacagccagg
    17521 acatcgcagc ccgcgtcggc cgcgacgtga tcgtcacgca catcggcgcg cgccgcgaca
    17581 acccgcgctg cgacaccggc gccacgcgcc tcagccgcga cgtcttcgcg gtggtgcgcg
    17641 accccgaggt cgacatcgtc gtcgagctga tcggcggctg cgacaccgcg cgcgaactgg
    17701 tgatggaggc catcgccgcc ggcaagcacg tggtgaccgc gaacaaggcg ctgatcgcgc
    17761 agcacggcaa ccagatcttc gccgccgcca gcgcgcgcgg cgtgaccgtg gccttcgagg
    17821 ccgcggtcgg cggcggtatc ccgatcatca aggccatccg cgaggggctg gcggcgaacc
    17881 gcatcgagtg ggtcgccggg atcatcaacg gcaccggtaa ctacatcctc tcggagatgc
    17941 gcgagaaggg gcgcagcttc gccgacgcgc tcgccgaggc gcaggcgctc ggttacgccg
    18001 aggccgatcc gaccttcgat gtcgagggca tcgacgcggc gcacaagctg gtgatcctcg
    18061 cctcgctcgc cttcggcatc ccgctgcagt tcgagaaggt ctacaccgag ggcatcggcg
    18121 cgctcgcccg ggaggacctc gcctacgccg ccgagctcgg ctatcgcatc aagcatctcg
    18181 gcatctgccg gcgcacgccg gcgggcatcg agttgcgcgt gcatccgaca ctcatcccct
    18241 acgaccgcct gatcgccaac gtcaacggcg tgatgaacgc gatcctggtc aagggcgacg
    18301 cggtgggtcc gacgctctac tacgggcccg gcgcgggatc cgagccgacc gcctcggcgg
    18361 tggtcgccga catcgtggat gtcgcgcgca cgctgaccgc cgatcccaat caccgcgtgc
    18421 cgcacctcgc gttccaggcc gatcgcctgt cctcggagcc ggtgctgccg atgtccgcgg
    18481 tgcagacctc gtattacctg cgcgtggccg cggtcgaccg ccccggcgtg ctgtggcgcg
    18541 tggcgcagat cctcagcgag gccggcatca gcatcgaggc gctgatccag aaggaggcgc
    18601 ccgagggctc ggagaccgcg accatgatcc tgctcaccag cgtgacgatc gagagccgca
    18661 tgagcgatgc catcgccgcg atcgaggcgc tggagagcac gcgcgggcgc gtcacgcgca
    18721 tccgcatgga aaagctcgac ggctgaggcc ggcattcaag gaggtttgcg ttgcgataca
    18781 tcagcacgcg gggccaggca cccgcactcg cgttcgagga cgtgatgctc accgggctcg
    18841 cgcccgacgg cggcctgtac gtgccggaaa cgctgccgcg cttcagcgcc gggcagatgc
    18901 gcgcgtgggc gaagctcgac tacgcggatc tcgccgccac gatcatgcag cccttcgtgg
    18961 agggtttcct ggcggaggac gagctgcgtg cgatcacgcg cgagacctac gccggattcc
    19021 gccacccggc ggtggcgccc ctggtgcagc tcggccacaa cgagtggctg ctcgagctgt
    19081 tccacgggcc gacgctggcg ttcaaggatt tcgcgctgca gctgctcggg cgcctgctgg
    19141 acgtgtcgct cgcgcggcgc caccagcgcg tcgtggtgct gggcgcgacc tcgggcgaca
    19201 ccggctcggc ggcgatcgag ggctgccgcc gctgcgccaa cgtcgacatc ttcatcctgc
    19261 acccgcacca gcgcgtctcc gaggtgcagc gccggcagat gaccacggtg cccggtgaca
    19321 acatccacaa catcgcggtg cagggcaact tcgacgactg ccaggagatg gtgaagcaga
    19381 gcttctcgga ccagtccttc ctgcccgagg ggcggcggct ggtcgcggtg aactcgatca
    19441 actgggcgcg gatcatggcc cagatcgtct actacttcca cgcggcgctg gcactgggcg
    19501 cgcccgagcg cgcggtgtcc ttcgcggtgc ccaccggcaa tttcggcgac atctacgccg
    19561 gatacctcgc caggcgcatg gggctgccgg tgcgccagct ggtcatcgcg accaacgcca
    19621 acgacatcct gcaccgcttc atgagcagca gccgctacgg caagtcggcg ctcgcgcaca
    19681 cgctgtcgcc gagcatggac atcgtgatct cgagcaactt cgagcgcgcg ctgttcgaat
    19741 tctacggacg cgacgccgcc gcgctcgccg acctgatgca gcgctttcgc agcagcgagg
    19801 tcgcggtgcc ggagtcggtg atcgcggccg cgcgcgcgga gttcgcgagt ttcagcgtcg
    19861 atgacgcgat gacctgcgcg acgatcgccg gggtctacga ggagtccgaa tacctgctgg
    19921 atccgcattc ggcgatcggc gtgcgcgccg cgcgcgaatg ccgcgacgat cccggcgtgc
    19981 cgatggtcac gctggccacc gcgcacccgg ccaaattccc cgaggcgatc gcgcgcgccg
    20041 gcgtggctcg cgaaccggcg ctgccgcact acatggcgga cctgttcgag cggcccgagc
    20101 gctgcgccgt gctgccgcac gaggtgcgcg ccgtgcagga gttcatccgc ggcaggatcg
    20161 cgtgagccgc gcatgaggcg cgcgatccgg cgccgcagcg cgcgcgccga cgcggcatct
    20221 gcgccaggcg tcagcccgtt gctggcacgg ctctacgccg cgcgcggcgt ggcggatgcg
    20281 agcgatctgg agcaggggct ggcgcagttg ctgccgcccg tgctgctcgg gctggagcag
    20341 gccgccacgc gtctggccga cgcgctggcg cgcgacgagc gcatcctgat cgttggggat
    20401 tacgacgccg acggcgcgac cagcaccgcg ctgggcctgc tcgcactgcg cgcgatgggc
    20461 tgcaccagcg cggacttcct ggtgccgaac cgcttcgagc acggctacgg gctgggtccc
    20521 gagatcgtcg cggtcgcggc cgcgctgcac ccgcggctca tcatcacggt cgacaacggc
    20581 atcgccagcc acgccggcgt ggcggccgcg gccgcgcacg gcatcgacgt gctcgtcacg
    20641 gaccatcacc tgcccggcga gaccttgccc gccgcctgcg cgatcgtgaa cccgaaccag
    20701 cccggctgcg ccttcccgag caagagcctg gccggcgtcg gcgtggtgtt ctacctgatg
    20761 ggcgcgctgc gcgcgcggct gcgcgccagc ggctggttcg ccgcgcgcgg gctcgcagaa
    20821 cccaacctcg gcgagctgct cgacctggtg gcgctcggca ccgtggccga cgtggtggcg
    20881 ctcgatgcca acaaccgcat cctcgtgcgc caggggctgc agcgcatccg cgccgggcgc
    20941 gggcgcccgg gcgtgcgcgc gctgctcgag ctcgccgggc gcaatcccgc gcgcgtggtc
    21001 agcgccgatc tgggtttcgc ggtcggaccg cgcatcaatg ccgccggacg gctcgatgac
    21061 atcggactcg gcatccgctg cctgctcgaa gaggagccgg cgcgcgcgcg cgagatggcc
    21121 gccgagctcg acgcgtggaa ccgcgaacgg cgcgacatcg agggtggcat gcagcaggaa
    21181 gcgctgcggc tgatggagca gggcgcgatt gccgaggacg cgctgccgtg gggcccctgc
    21241 ctgtaccacc ccgactggca ccagggcgtg gtggggctgg tcgcctcgcg catcaaggag
    21301 aagcaccacc gcccggtgat tgccttcgcg cccggtggcg cgggcgagtt gaagggttcc
    21361 gcgcgttcga tccgcggttt ccatatccgc gacgcgatcg ccgccgtcgc cgcggcggcg
    21421 ccggggctgg tcgagcgctt cggcggccat gcgatggccg cggggctcac gctggcggcg
    21481 gagcgcttcg aggagttcgc gcgcgccttc gatgccgagg tgcgtcgtca cctcgaagcg
    21541 agcgacctcg agagcgtgct ggagagcgac ggcgaactgg gtgccggcga gttctcgctg
    21601 gcgcaggccg aggagctgcg cgaggcgggg ccctggggcc agcacttccc cgagccgctg
    21661 ttcgacggcg agttctggct gcgcagccag cgcctgctgg gcggcaagca cctgaagctg
    21721 gtggtctcgc cggtggcggc gccacagctc gagctcgagg cgatcgcgtt caacatcgat
    21781 gctgccctct ggcccgatgc gcg
//
LOCUS       DAORUQ010000031        21568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2016_length_21568_cov_6.651048, whole genome shotgun sequence.
ACCESSION   DAORUQ010000031 DAORUQ010000000
VERSION     DAORUQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21568
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_21568_cov_6.651048"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..563)
                     /locus_tag="PLW13_04655"
     CDS             complement(171..563)
                     /locus_tag="PLW13_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69691.1"
                     /translation="MPIVATESWTLELPEEWSAQEDEDVVVIEDEDGVSCLEISALVL
                     ESGAVGEEDLAEFSRDLLDEGLKPQAVRVAGWSGKLFEHDDEEFHWREWFLRQGAHFV
                     YAGYHCLREHAGMDDAAIAEILATLEPC"
     gene            complement(575..1021)
                     /gene="rimI"
                     /locus_tag="PLW13_04660"
     CDS             complement(575..1021)
                     /gene="rimI"
                     /locus_tag="PLW13_04660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQY69692.1"
                     /translation="MMPEPRPAAADDAEAIAALDARVNPSPWSADAVRETLARAGGFV
                     IAPPGAPLAGFALFAQAADECEILDIAVDPAARRNGLGARLLAAALAAAATRGARRCF
                     LEVRASNAGAQAFYRAAGFVEDGRRKDYYRSGQGREDALLMSRALP"
     gene            complement(1011..1739)
                     /locus_tag="PLW13_04665"
     CDS             complement(1011..1739)
                     /locus_tag="PLW13_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69693.1"
                     /translation="MEEGLRLAYLAAMDVPVWLLRGSEPVPVFDEEPPPAREQAAPVA
                     ADTGPVRLARDLLGEDSGPRAGVTRAATPERSGAPARTAPRVAAQPTPSLLLVEAGRY
                     LFIEASSEQGGDRRVVELIGAIALALGGEPVAPRAQRFDLQALGALADRAQARDVLLG
                     RLARLGESQTVEHVVLLGERPAAVLLGWDEARFAGRIARVQRVPGLEPGVLVTLSCAQ
                     MLADAGLKRAVWQELCAARARHDA"
     gene            complement(1750..3294)
                     /locus_tag="PLW13_04670"
     CDS             complement(1750..3294)
                     /locus_tag="PLW13_04670"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQY69694.1"
                     /translation="MSKDRLIIFDTTMRDGEQSPGASMTREEKIRIARSLERLNVDVI
                     EAGFAIASPGDFEAVKAVAETVKNCTVASLARAAYRDIEEAGAALKNAVRPRIHTFIA
                     TSPIHMQYKLQMTPEQVLDQAVRSVKQARNHTDDVEFSCEDASRSEFDFLCRVIEAVI
                     AAGAGTINLPDTVGYGEPGQYGEFVGRLIRTVPNADKAIFSVHCHNDLGLAVANSLSA
                     VMHGARQVECTINGLGERAGNASLEEIVMAVRTRADIFPVETRIVTEEIVPASRLVSS
                     ITGFPVQPNKAIVGANAFAHEAGIHQDGVLKHRETYEIMRAQDVGWSTNRLVLGKHSG
                     RAAVRARFEELGIGFETPEELESAFKRFKELADKKHEIFDEDLQAIAGTVSSEDEAER
                     YQFVALEAISHTGEKPRATVTLSIDGVERRSYAEGDGPVDAVFKAIENEVHSGTKLLL
                     FSINAITTGTDSQAEVMVRLERDARIVNGAGADTDIIVASAKAYLHALNVMGGAVRAH
                     PQIGGI"
     gene            3750..4841
                     /locus_tag="PLW13_04675"
     CDS             3750..4841
                     /locus_tag="PLW13_04675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY69695.1"
                     /translation="MLGLIRFALATAVLLSHVPGFHWALNPGVMAVICFYTISGYLMR
                     RSIERFGRYSARPARAFLADRVLKLFPQYLVALAATVALLWAWGPSPGFWLLQQEPTP
                     LKVLLNALLLPANYIFGPFVIPQLLPHPLVPPAWSLATEFHFYLLLPLLFRWRALLLV
                     LLPATLAVQLFGFVHESPTLNSDTLSYRYIFGVLPVFLMGYLYAEGGTTSRRIAFALW
                     FLYVLLAGCALAGVAIHQQRAREITLGATMALPLLALALASGRRALPAALKRWDERLG
                     NLAYPVFITHCLAFFVVEHLTGMPVSATAGFVTSAAAVCLAGSLALEQMQRRVERLRI
                     GLRGFASMKDAAPQPRAGAEPDARIASRD"
     gene            4912..5853
                     /locus_tag="PLW13_04680"
     CDS             4912..5853
                     /locus_tag="PLW13_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69696.1"
                     /translation="MTQGKRILVTGGAGFIGSHLCRRLLARGDEVVCVDNFYTGRKSN
                     VLDLLGNPLFEILRHDVTFPLYAEVDQIYNLACPASPIYYQFDPVQTTKTSVHGAINM
                     LGLAKRLKAPILQASTSEVYGDPEVHPQHESYWGRVNPVGPRSCYDEGKRCAETLFFD
                     YYRQHRLAIRVIRIFNTYGPNMQPDDGRVVSNFIVQALRGEDLTIYGDGTQTRSFCYV
                     DDLVEAMIRMMDNAENDPGPVNLGNPVESTILQIAETIRRLTGSSSRIVFRPLPVDDP
                     RQRQPDISRARELLGWEPATGLEDGLAKTIAYFRKTL"
     gene            5850..6539
                     /locus_tag="PLW13_04685"
     CDS             5850..6539
                     /locus_tag="PLW13_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY69697.1"
                     /translation="MTLSIVIPCYNEKSTIEDLIEAVRNAPGGDKEIIVVDDCSTDGT
                     TELLRERLAARIDRLIHQPVNRGKGAALRTGIAAATGDIVIIQDADLEYDPRQYDVLI
                     APIVQDKADVVYGSRFIGSEPHRVLYFWHRLGNGLLTLLSNMLTNLNLTDMETCYKVF
                     RRELIQGIAIEEDRFGFEPEITAKIARSRCRIYEVGISYHGRTYEEGKKIGWKDGVRA
                     IYCILKYNLLR"
     gene            6547..6933
                     /locus_tag="PLW13_04690"
     CDS             6547..6933
                     /locus_tag="PLW13_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQY69698.1"
                     /translation="MQIVRYFFVGGVAAVVDIAVFAALINLLGMHYLVAGMAGFLLAT
                     GVNYVLSVRHVFAAGARFSRRKELLAVYAVSAVGLLWHQAALYAGVEMLGMNVYAAKI
                     GAIALVFFWNFFLRKHFVFAPAARKT"
     gene            complement(6940..7614)
                     /locus_tag="PLW13_04695"
     CDS             complement(6940..7614)
                     /locus_tag="PLW13_04695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY69699.1"
                     /translation="MGRIKAANSAFSEIYNKDVWALDSGEWHAQQAGYEPLESCYARF
                     VALLQDFMRHNRVRSVVEFGCGFWSYAREIDWSGIEYDGFDIVDGPVRWNRDHHGADN
                     IRFHRLEDGTRLPPADLLISKDVLQHLPIADIRYYTDIFRRNYRFSIIASGVFPDHDT
                     NSEIAPGECRSLRLDLPPFDIPCAVLQRWEYIEFGKPVVKDVCLVTGLPESAAAGGAI
                     VRDVDA"
     gene            complement(7665..8798)
                     /locus_tag="PLW13_04700"
     CDS             complement(7665..8798)
                     /locus_tag="PLW13_04700"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008078905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY69700.1"
                     /translation="MKGRIDDIEVLRAIAVIFVCIEHMHMNLFAWVGGPLHTTFFGWT
                     GMWSGVDLFFVISGFVIARSLVPAITAAESTHERLVTTLAFWIRRAWRLIPSAWLWLA
                     VILVACVFFDRSGAWGSLRANLEGALTAVFNVNNLRVMLTYGKFEAGATFPYWSLSLE
                     EQFYLVLPFVVWLCGKRLPLVLAVVVVLQLVFPRTPLGTIMRCDALALGVLIAIWSRL
                     DSYRLFEPRFMANRWVRIALVSILAGGLALAGGEDMHVPVPISLVAFIGAILVFMASY
                     DRNYIMAPGWIKKLLLWVGSRSYAIYLIHIPAYFATREIWFRLQPPGIGFDPNFTGKF
                     LVTAVVLMVVFAELNYRLVEMPLRRRGAEIATRFRATHLQPAA"
     gene            complement(8856..9203)
                     /locus_tag="PLW13_04705"
     CDS             complement(8856..9203)
                     /locus_tag="PLW13_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQY69701.1"
                     /translation="MMTNKVYALAILTILLNAVAQILMKAGASASAADAAPLLRAWGL
                     IGAALLIYVIGMALWIYVLRQVELTLVYPFMALSFVVVPLLSVLFLGERVDLPYMAGV
                     VLIMGGIYLCSAV"
     gene            complement(9231..9953)
                     /locus_tag="PLW13_04710"
     CDS             complement(9231..9953)
                     /locus_tag="PLW13_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69702.1"
                     /translation="MFEQFPKTRPALPEEIARIYVSWYRENREGGSAAASAAQAVEAW
                     LHRHLAADLRPGASGVSTLELGAGTLNQLGFEPACGPYDIVEPFAELFRDSPALARVR
                     HVHADIREVPAEARYDRITSSAVLEHLCDLPEVVARSCLLLAEGGTFRASIPSEGTWV
                     WYLSWRLSTGLEFWLRHRLDYGRIMRHEHVNTAREIEAVLRYFFGEVSMKVLGLSRGI
                     SLFQHFACAQPRRDRARAYLHG"
     gene            complement(9953..12253)
                     /locus_tag="PLW13_04715"
     CDS             complement(9953..12253)
                     /locus_tag="PLW13_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69703.1"
                     /translation="MTAARVATTQRGPDLAAAALILAASLALFAPALFGPRAILIGDS
                     LFHGIPMLHYLREVLYGDYSLLWTDLVYVGHPVFAESQGGFLNPLNLLVAALFEPLTG
                     VDVYHALCTFLGGLGVYLLCRELGCRPVSALFGAAATIGSTMWLTLQGNLTISNVAMA
                     VPWVMLAFERLLRRADVPGGLWLALAMSYLVLAGYPHVVQGIVVFLFLSLLPVAFGWA
                     RRGEFVARVRALLGPALVAVVLTLGLCAAQLLPMLELAGESHRAAGVDYVRFQPQTFF
                     RGFLFTDMVPEELTPNFTLLGSMAVSALFVAGVFSARGARAPGVVLATLSLCYLGFGA
                     QAPGFEFIHRHGLVPGMRFFRLMTPYFYIGIVGAGVVAALGVEALADAGRPARRRVAL
                     LLGSALPVLGAALALHTAQVHWWHYASLALCAGCAVALILARREALLPAALLIALAVE
                     IGVLRAQAIYFAPTDLVARPGVLQQTCWRAGACAANTADLSINTVIVGFAHARSREAP
                     LRIPQALAALSPMTNMLWDVPAINGAMALQLRRSKLATDLVREEMLQGTPRAPGTRIT
                     DILGVRYLTLGGFAPAADAALRSGEALAPLSENPAARGRWQFYRDAVFVESADAALAR
                     LRAAEPCANLPLVIEGLPSPDDPRSACDAAPAPETARIEVRAATADDMRIEVRTDAPG
                     WLFVADSWYPGWRARVNGEDRTVYPAQLAGKAVRLDAGLNHVRLRYAPLSFRIGVCIS
                     LAAVLAFALIALRARVRRSNVQEATA"
     gene            complement(12250..13227)
                     /locus_tag="PLW13_04720"
     CDS             complement(12250..13227)
                     /locus_tag="PLW13_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY69704.1"
                     /translation="MTSIAVVIPAYRVARQIVPLIARIPPLVTRIIVVDDACPEHSGA
                     LVRQQVNDPRVRLIVHAQNQGVGAAVISGVRAALEGGADIVVKLDGDAQYDPELIPYL
                     VQPIIAGNADVTKGNRFFFLEDVAQMPALRLFGNAVLSFVNKAVSGYWDVMDPTNGFI
                     AIHAEVLHMLPLDKLAPRYFFESDLLFRLGTLRAVVQDMPVRAHYADEQSSLRIGATA
                     LGFPLKYASRLFKRVFYNHFLRDFNLGSVSLVVGLLLLGSGGIFGALEWYRSYATGHP
                     ATAGTVMLVALQLIVSLNLLILFLTVDIGNVPRRVLHRSVLRQSGRRNP"
     gene            complement(13238..14188)
                     /locus_tag="PLW13_04725"
     CDS             complement(13238..14188)
                     /locus_tag="PLW13_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69705.1"
                     /translation="MGKWIQDRAPGGDSAVAAIEARLAALESGLARLVAAQEQAAQPN
                     YVDRGTQQLLAMQYRRDAAAGVVHDFGSVEFRNHSQNGEDGILHYLFSVIGTTSRYVV
                     EMCAGNGRECNAANLIINHGWHALLCDGSEENIRIANDFYWRHPDTMRIPPAICQAWL
                     TAENVNEVIARYGFDREIDLLSIDVDGNDYWLWRAITAANPRVVIIEIQAGWMADAAV
                     TVPYDPEFCVRKLVDPVLQTEVDYCGASLPAMVKLGREKGYRLVGANRYGFNVVFVRN
                     DIAAELLPEIPAEQCFGHPVARWQYGRVQPLLRREAWVEV"
     gene            complement(14235..15110)
                     /locus_tag="PLW13_04730"
     CDS             complement(14235..15110)
                     /locus_tag="PLW13_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY69706.1"
                     /translation="MRAKLRQLLRRRAPAPPDGDAGERHAVDDADPRRDPRHCGLVDM
                     VMSDWLNAETGELFTGFALGPQDELLDVGCGDGLAVMFAAQQGARTTFCDLDPAKVRS
                     LSDRLSERGLTRHAGLVASGDRLPLPDGCMSRIIATEVLEHVEDPAAVLREMVRVGAP
                     GAKYLISVPDAASERFQQPFAHASYFAAPNHVRIIEPRELEELAESCGLQVEGRGQWG
                     FYWFLWMNFFWASQREAGDDATAMDRIRPPYHPLLQSWTETWTRLLELPETEGLRRAL
                     NGLMPKSRVIVARRK"
     gene            complement(15095..16414)
                     /locus_tag="PLW13_04735"
     CDS             complement(15095..16414)
                     /locus_tag="PLW13_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQY69707.1"
                     /translation="MIFLVHVPFTADSIEGEVGRADYSYYFVMQAYLPVLQALGRVVE
                     IADPLREADAWYRDCAASGEYCVLLCFAPPHRVPTRLECPVVPVFAWEYDSLPDEPWG
                     DRRETDWLRVLAMLGSAITHSQFTARVVRRAIRPDYPVVSVPAPVWDRFAPLAARRRE
                     TSGADRSVELDGTLMDSRACDFTEPPQALIAAHPRRRQQLRLDGVVYTAVFCPLDGRK
                     NWEDLVSAFCWAFRDVADCTLVLKLVHHDRDKALEEVLALLRKLPRTPARIVLLHAHL
                     DDAQYARLICATDFAVNSSLGEGQCLPLMEYMSAGVPAIAPAHTAMLDYLDGECGFPV
                     RAHEELYHWPQDMGLILRTRRFRPEWEGLRRAYATSRELRGADPDAYARMAEAASRRL
                     QQHCSQDVAREHLARFFAAHAAAIGYARRDARAAALAATGNAPCARS"
     gene            complement(16411..18585)
                     /locus_tag="PLW13_04740"
     CDS             complement(16411..18585)
                     /locus_tag="PLW13_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69708.1"
                     /translation="MGRPMHFLLYSRINAGNIGRSLGAPEYSYYFLLKEFRVAFERLG
                     AVTVVEDPAAEADALFDRFSAQGQQCVLIAFTAPQNLPDVRRCPVVPVIAWEFERIPD
                     EPWGGNPRNDWRVALAGCARAITLSEHAVAALQRAMGAQFPVLAVPVPLWERMAGVRE
                     RLASGREDGQPGICVDGAVLDTRDFERGPDRLRCNRPYAAYALELWDGQAHALDFRLL
                     SPDTGALLGFYRPEPWGAWSRNAEIAIALPWLLHGEVAIELDVRAYGRNQGRPLVAAL
                     GDAFQPLCIGGGEELHTLRFRLGRPACMLHIMGIDPRPLAGAAEERSIGFGITGLRLL
                     PAVEAPSDGTIRLELRAGCPESALLQEFWAPEPWGAWSASTAPWLMLPRPVNGRIAMR
                     VEMVGYAQNAGAPLSLYLGEQTRTIVPKAEAEVIALEFDLTEPAQVVGFTGVRKLPAS
                     EPEDARTLGIGLCRLAIDELDILDAMPLPPAPARAVAAHVRQALTLDGVVYTSVLNPR
                     DDRKNWDMLVSAFCTAFAGRDDVTLLLKMTQQLQRSYIFELHSLMQRLPAFACRVVVV
                     HGFLDEQDYGQLIGRTDFYVNVSKAEGLCIPLMEFMSCGKPALAPRHTSLLDYLDDAN
                     SIAIEATTEPCIWPHDERALLRTLQYRVSWESTVAAFRHSLGVYREDPQAYRQMGVAA
                     AAAMQRYCTVERVTDSIGAFLGVAPAAGAQREAGRLRPGADA"
     gene            complement(18589..19851)
                     /locus_tag="PLW13_04745"
     CDS             complement(18589..19851)
                     /locus_tag="PLW13_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69709.1"
                     /translation="MSHTVEVIGLGKAYRHYPNRWSRLAEWLLPFGGPRHQQSWALRD
                     VSFTVSPGEAVGIVGFNGAGKSTLLRIITGTTRATEGEVRLHGRVAALLELGMGFHGE
                     FTGRQNVMMNGQLLGLERAELEELMPAIEDFAEIGAYFDEPLRTYSSGMAMRLAFSLA
                     TARRPELLIVDEALSVGDAYFQHKSFSRIREFREQGTTLLIVSHDRLSIQALCDRAIL
                     LHEGRLLREGEPEIVMDFYHALMADRTFAHIRQELNAQGVVQTFSGTGEATIVSVTLQ
                     NAGGATTDAAKVGEEVVMSVRFRVHVDLPTLVVGLMIKDRLGHEMFGINSHRLSIPIE
                     NLAAGSEHVFRLRFVMNLGEGHYSVTTALTRSDAHVDRTYEWRDRALIFYVINVAHPR
                     FVGCNWLAPVASLDAPDAAGRVADATGS"
     gene            complement(19848..20645)
                     /locus_tag="PLW13_04750"
     CDS             complement(19848..20645)
                     /locus_tag="PLW13_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69710.1"
                     /translation="MLIQLIRAAWRSRGFIVSSVRREFAARYGNSLLGSAWAVINPLA
                     MVAVYMLIFSQVMQARLPGETGTLAYGIFLCAGILSWGLFSEIIQRGMTVFLDHANLL
                     KKMNFPRICLPLIVLLNAGMNFAIIYGLFLLLLLVTGNFPGAYLLAMLPVLAILCVFA
                     LGLGIVAGVLNVFFRDAGQFFGILLQFWFWFTPIVYPLSILPEAIRPIVLANPLTPLM
                     LSQQAIALGQGWPQWSALVAPALCATLALVVALALFRRRADEIVDEL"
     gene            complement(20802..21563)
                     /locus_tag="PLW13_04755"
     CDS             complement(20802..21563)
                     /locus_tag="PLW13_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY69711.1"
                     /translation="MWCVLASVYAAVTIAVPSFNQGRYLGAALESALDQGVAVEVFVA
                     DGGSGDDSVAVIERFAPRLAGWRSHADRGQAAAINECIARGSAPYVCWLNSDDLLLPG
                     ALATLIAALDAAPAAPMAYARAWNLVEATGRRDPVWVEPFDERRLALRCIVSQPATLI
                     RRSAWEAVGGLDESLHMVMDYDLWWRLYRRCGAPCFVDAVTGVNREHAGTKTRRNRAR
                     HYREAIAVVRRHYGRVPLKWWLYQPYAVWFKALTG"
BASE COUNT         3587 a         7669 c         7180 g         3132 t
ORIGIN      
        1 tgcggggcct tgcgccgcag gacgcggcgc ggagctacag ggacgtactc gtgcgtgccc
       61 cgcaggacaa tagcgggcag cacagccagc gacgcaccaa gccgctcgct cgcaaggtaa
      121 tggcttttgc gaacaccctc tgaagtgaaa ccggacccac ggcgtgcggt tcagcacggc
      181 tccagcgtcg cgaggatctc ggcgatagcc gcgtcgtcca tgccggcgtg ctcgcgcagg
      241 cagtggtagc cggcgtagac gaaatgcgcg ccctggcgca ggaaccactc gcgccagtgg
      301 aactcctcgt cgtcgtgctc gaagagcttg ccgctccagc cggcgacgcg caccgcctgc
      361 ggcttcaggc cctcgtcgag aagatcgcgg ctgaactcgg ccagatcctc ctcgcccacg
      421 gcgccgctct ccagcaccag cgccgagatc tcgaggcagc tcacgccgtc ctcgtcctcg
      481 atcaccacga cgtcctcgtc ctcctgcgcc gaccactcct cgggcagttc cagcgtccac
      541 gactcggttg cgacgatcgg catcggtgtc tccctcatgg cagcgcgcgg ctcatcagca
      601 gcgcgtcctc gcgcccctgg ccgctgcggt agtaatcctt gcgccgcccg tcctcgacga
      661 agccggccgc gcgatagaaa gcctgcgcac cggcgttcga ggcgcgcact tccaggaagc
      721 aacgcctcgc cccgcgcgtg gcggcggcgg ccagcgcggc agccagcagg cgtgcgccga
      781 gcccgttacg ccgcgcggcc ggatcgaccg cgatatcgag gatttcgcac tcgtcggcgg
      841 cctgggcgaa cagcgcgaac ccggccagtg gcgcgcccgg cggcgcgatc acgaaaccac
      901 cggcgcgcgc cagcgtttcg cgcacggcat ccgcgctcca cggcgaggga ttgacccgcg
      961 catccagcgc cgcgatcgcc tcggcgtcgt ccgcggcggc cggtcgcggc tcaggcatca
     1021 tggcgggcgc gcgccgcgca gagctcctgc cagaccgcgc gcttgagccc cgcgtcggcg
     1081 agcatctgcg cgcacgacag cgtgaccagc acgccgggct ccaggcccgg cacgcgctga
     1141 acgcgcgcga tacgcccggc gaaacgggct tcgtcccatc ccagcagcac cgcggccggg
     1201 cgctcgccga gcagcaccac gtgttcgacg gtttgcgatt cacccagcct ggccaggcgc
     1261 cccagcagca cgtcgcgcgc ctgcgcgcga tcggccagcg caccgagcgc ctgcaggtcg
     1321 aagcgctgcg cccgcggcgc cacgggctcg ccgccgagcg cgagcgcgat cgcgccgatc
     1381 agttccacga cgcgccggtc cccgccctgc tcgctgctcg cctcgatgaa gagatagcgc
     1441 cccgcctcga ccagcagcag gctgggcgtg ggctgcgccg ccacgcgcgg cgcggtgcgc
     1501 gccggcgcgc cgctgcgctc gggcgtcgcc gcgcgcgtca ccccggcgcg cggcccgctg
     1561 tcctcgccga gcaggtcgcg cgcgagacgc accggaccgg tatcggccgc aaccggcgca
     1621 gcctgctcgc gcgccggcgg cggctcctcg tcgaacacgg gcaccggttc gctgccgcgc
     1681 aacagccaga ccggcacgtc catcgcggcg aggtaggcga ggcgcaggcc ctcctccatt
     1741 gcggcgcgct cagataccgc cgatctgcgg atgcgcgcgc accgcgccgc ccatcacgtt
     1801 cagcgcatgc aggtaggcct tcgccgaggc gacgatgatg tccgtgtcgg cgccggcgcc
     1861 gttcacgatg cgcgcatcgc gctccaggcg caccatcacc tcggcctgcg aatcggtgcc
     1921 ggtggtgatc gcgttgatcg agaacagcaa cagcttcgtg ccgctgtgga cctcgttctc
     1981 gatggccttg aacaccgcgt ccaccggccc gtcgccttcc gcgtagctcc ggcgctcgac
     2041 gccgtcgatg ctcagcgtca cggtcgcgcg cggcttctcg ccggtgtgcg agatggcttc
     2101 gagcgcgacg aactggtagc gctcggcctc gtcctcgctg gacacggtgc cggcgatcgc
     2161 ctgcaggtcc tcgtcgaaga tctcgtgctt cttgtccgcg agctccttga agcgcttgaa
     2221 cgcgctctcg agttcctcgg gcgtctcgaa gccgatgccg agttcctcga aacgcgcgcg
     2281 caccgcggcg cggcccgagt gcttgccgag cacgaggcgg ttggtgctcc agcccacgtc
     2341 ctgcgcgcgc atgatctcgt aggtctcgcg gtgcttgagc accccgtcct ggtggatgcc
     2401 ggcctcgtgg gcgaaggcat tggcgccgac gatggccttg ttgggctgca ccgggaaacc
     2461 ggtgatcgag gacaccagcc gcgaggccgg gacgatctcc tcggtcacga tgcgcgtctc
     2521 gaccgggaag atgtcggcgc gggtgcgcac cgccatcacg atctcctcga gcgaggcgtt
     2581 gccggcgcgc tcgccgagcc cgttgatcgt gcactccacc tggcgcgccc cgtgcatcac
     2641 cgccgacagc gagttcgcca ccgccaggcc gaggtcgttg tggcaatgca ccgagaagat
     2701 cgccttgtcg gcgttcggga cggtgcggat cagtcgcccg acgaactcgc catactgccc
     2761 cggctcgccg taaccgacgg tatcgggaag attgatcgtg ccggcgccgg cagcgatcac
     2821 ggcctcgatg acgcggcaga ggaagtcgaa ctccgagcgg ctggcgtcct cgcaggagaa
     2881 ctccacgtca tcggtgtggt tgcgcgcctg cttcaccgag cgcaccgcct ggtcgagcac
     2941 ctgctcgggc gtcatctgca gcttgtactg catgtggatc ggcgaagtcg cgatgaaggt
     3001 gtggatccgc ggtcgcacgg cgttcttcag cgccgcgccg gcttcctcga tgtcgcggta
     3061 ggccgcgcgc gcgaggctgg cgacggtgca gttcttgacc gtctcggcca ccgccttgac
     3121 ggcctcgaag tcgccggggc tcgcgatcgc gaagccggcc tcgatcacgt ccacgttcag
     3181 ccgctccagc gagcgcgcga tgcggatctt ctcttccctg gtcatcgacg cgccggggct
     3241 ctgctcgccg tcacgcatcg tcgtatcgaa aatgatcaac cggtccttgc tcatggcttg
     3301 tcctcgtgtc cgggcgggcg cgcagggggc acccatcctg gtgaatttgt cggggtaacg
     3361 gaaatgtgga cgaacggtat actgcccctc agggcagcag cagggcgaga cgcagcgcgc
     3421 gcagggagcg cggcgcggaa agactggcga tgtggccctg gaagctcatc gtgctacgga
     3481 tctgtgacgc aatgccgcaa agcggcgatg gagccgatat tagtcaaagc ggcgcggcgt
     3541 tgccaagccc cgcagccgcc ggcagtgaat tgttgcgctg tgcagcgcgc tgcgcgccgg
     3601 cttgtcacct ccgggcccct gccttaaggt gcgccatccc gggtggagcc ccagatgccg
     3661 cgcacgccac cagccatgac cgttgaactc ccgcggcgaa gcgccgcgcg gcgccctgcg
     3721 cggcgcgcac ccgatgcggg aggcacgcct tgctcggcct gatccgcttc gcgctggcca
     3781 ccgcggtgct gctctcgcac gtgcccgggt tccactgggc gctgaatccc ggcgtgatgg
     3841 ccgtgatctg cttctacacc atcagcggct acctgatgcg gcgcagcatc gaacgcttcg
     3901 ggcgctactc ggcgcggccg gcgcgcgcct tcctcgcgga ccgcgtgctg aaactgttcc
     3961 cgcaatacct cgtggcgttg gcggcgaccg tggcgctgct gtgggcctgg ggcccctcgc
     4021 ccggattctg gctgctgcaa caggagccca caccgctgaa ggtgctgctc aacgcgctgc
     4081 tgttgcccgc caactacatc ttcgggccct tcgtgatccc gcaactgctg ccgcaccccc
     4141 tggtgccgcc ggcctggtcg ctcgccaccg agttccattt ctacctgctg ctgccgctgc
     4201 tgttccgctg gcgggcgctg ctgctggtgc tgctgccagc cacgctcgcg gtgcagctgt
     4261 tcggattcgt gcacgaatcg ccgacgctca acagcgacac cctgagctat cgctacatct
     4321 tcggcgtgct gccggtgttc ctgatgggct atctctacgc cgagggcggc accacgagcc
     4381 gccgcatcgc attcgcgctg tggtttcttt atgtcctgct cgccggctgc gcgctggccg
     4441 gcgtggcgat ccaccagcag cgcgcgcggg aaatcacgct cggcgccacc atggcgctgc
     4501 cgctgctcgc gctggcgctg gccagcggca ggcgcgccct gccggcagcg ctgaagcgct
     4561 gggacgagcg actgggcaac ctcgcctatc ccgtcttcat cacgcactgc ctcgcgttct
     4621 tcgtcgtcga gcacctcacc ggcatgccgg tcagcgcgac cgcgggcttc gtgaccagcg
     4681 ccgcggccgt gtgcctcgcg ggctcgctgg cgctcgaaca gatgcagcgc cgcgtcgagc
     4741 gcctgcgcat cggcctgcgc ggattcgcct ctatgaagga cgccgcgccg cagccgcgcg
     4801 ccggcgcaga gcccgatgct agaatcgcga gccgggactg atcccgacgg gcccacgcgc
     4861 cggcgccagc cggactgccg gctgttcatt ccgttttgac tggatcggcg catgacgcaa
     4921 ggcaagagaa ttctggttac cggcggcgcg ggattcatcg gatcgcacct gtgccggcga
     4981 ctgctggcgc gcggcgacga ggtcgtgtgc gtcgacaact tctataccgg gcgcaagtcg
     5041 aacgtgctcg atttgctcgg caatccgctg ttcgagatcc tgcgccacga cgtcaccttt
     5101 cctctctacg ccgaagtcga ccagatctac aacctggcct gccctgcctc cccgatctat
     5161 taccagttcg atccggtgca gacgacgaag acctcggtac acggcgccat caacatgctc
     5221 gggctggcca agcggctgaa ggcccccatc ctgcaggcct cgacatccga ggtctacggc
     5281 gaccccgagg tccatcccca gcacgaatcc tactggggac gcgtcaaccc ggtcgggccg
     5341 cgcagctgct acgacgaggg caagcgctgc gccgagacgc tgttcttcga ctactaccgc
     5401 cagcaccggc tcgcgatccg cgtgatccgg atattcaaca cctacggccc gaacatgcag
     5461 cccgacgacg gccgcgtcgt cagcaacttc atcgtgcagg cgctgcgcgg cgaggacctc
     5521 accatctacg gcgacggcac gcagacacgc tcgttctgct atgtggacga cctggtcgaa
     5581 gcgatgatcc gcatgatgga caacgccgaa aacgatccgg gccccgtcaa tctcggaaat
     5641 cccgtcgaat cgaccatcct gcagatcgcc gagaccatcc gccggctcac cggctcgtcc
     5701 tcgcgcatcg ttttccggcc gctgccggtg gacgacccgc gccagcgcca gccggacatc
     5761 tcgcgggcgc gcgagctgct cggctgggag cccgcgacgg ggctggagga cggcctggcg
     5821 aagaccatcg catacttccg gaaaacattg tgacgctcag catcgtcata ccctgctaca
     5881 acgaaaaatc gaccatcgag gatctcatcg aggccgtgcg caacgcaccg ggcggcgaca
     5941 aggagatcat cgtcgtcgac gactgctcca ccgacggcac caccgagctg ctgcgcgagc
     6001 ggctcgccgc ccgcatcgac cggctgatcc accagcccgt gaaccgcggc aagggtgccg
     6061 cgctgcgcac cggcatcgcg gctgcgaccg gcgacatcgt gatcatccag gacgcggacc
     6121 tcgaatacga tccgcggcag tacgacgtgc tgatcgcacc gatcgtgcag gacaaggccg
     6181 acgtggtcta cggttcgcgc ttcatcggct cggagccgca ccgcgtgctg tatttctggc
     6241 atcgcctcgg caacggcctg ctgacgctgc tatccaacat gttgaccaac ctgaacctaa
     6301 ccgacatgga aacctgctac aaggtgttcc ggcgcgaact catccagggc atcgcgatcg
     6361 aggaggaccg cttcggcttc gagcccgaga tcacggcgaa gatcgcacgc agccgctgcc
     6421 gcatctacga ggtgggcatc tcctatcacg ggcgcaccta cgaggaaggc aagaagatcg
     6481 gctggaagga cggcgtgcgc gccatctact gcatcctgaa atacaacctg ttgcggtagc
     6541 gacccggtgc agatcgtgcg ctatttcttc gtcggcggcg tcgccgcggt ggtggacatc
     6601 gcggtcttcg ccgccctgat caacctgctc ggcatgcatt acctggtagc cggtatggcg
     6661 ggcttcctgc tcgccaccgg ggtcaactac gtgctcagcg tgcgccacgt gttcgcggcc
     6721 ggggcgcgct tttcgcggcg caaggaactg ctggcggtct atgcggtcag cgcggtcggg
     6781 ctgctgtggc accaggccgc gctgtacgcc ggcgtcgaga tgctcggcat gaacgtgtac
     6841 gccgccaaga tcggcgctat cgcgctggtg ttcttctgga atttcttcct gcgcaagcac
     6901 ttcgtgttcg cgcccgccgc gcgcaagacc tgacgcggct caggcgtcga cgtcgcgcac
     6961 gatcgcaccg cccgccgcgg cgctctccgg caggccggtc acgaggcaca cgtccttgac
     7021 cacgggcttg ccgaattcga tgtattccca gcgctgcagc accgcgcagg gaatatcgaa
     7081 gggcggcagg tccagccgca gcgagcggca ctcgccgggc gcgatctcgc tgttggtgtc
     7141 gtgatccgga aagacgccgc tggcgatgat cgagaagcga tagttgcgcc ggaagatgtc
     7201 cgtgtagtag cggatgtccg cgatgggcag gtgttgcagc acatccttgc tgatcagcag
     7261 gtccgcggga ggcaggcgcg taccgtcctc gaggcggtgg aagcggatat tgtcggcgcc
     7321 atggtggtcc cgattccagc gcaccggtcc gtcgacgatg tcgaagccgt cgtattcgat
     7381 gccgctccag tcgatttccc gcgcgtagct ccagaaaccg cagccgaact ccaccaccga
     7441 gcgcacgcga ttgtggcgca tgaagtcctg cagcagcgcg acgaaacggg cgtagcagga
     7501 ctccagcggt tcgtagcctg cctgctgcgc atgccattcc ccggagtcga gcgcccacac
     7561 gtccttgttg tagatctcgc tgaatgcgct gttcgcggcc ttgatgcgac ccatgtcaga
     7621 tgctccaagc tttacgtgag cgagtacagc taaaccggcc ttcctcaggc agcgggctgc
     7681 aggtgcgtgg cgcgaaagcg tgtcgcgatc tccgcgccac gacggcgcag cggcatctcg
     7741 accagccggt aattgagctc cgcgaacacc accatcagca ccaccgccgt gaccaggaac
     7801 ttgccggtga aattcggatc gaagccgatg cccggcggct gcaggcggaa ccagatctcg
     7861 cgcgtcgcga aataggccgg gatgtggatc aggtagatcg cgtaggaacg gctgcccacc
     7921 cacagcaaca gtttcttgat ccaccccggc gccatgatgt agttgcggtc gtaggatgcc
     7981 atgaacacca gtatcgcgcc gatgaaggcc accaggctga tcggcaccgg cacgtgcatg
     8041 tcctcgccgc cggcgagggc caggccgccg gcgagtatgc tcaccagcgc gatacgcacc
     8101 cagcggttcg ccatgaaacg cggttcgaac agccggtagc tgtccaggcg cgaccagatc
     8161 gcgatcagca cgccgagtgc cagcgcgtcg cagcgcatga tggtgccgag cggcgtgcgc
     8221 ggaaacacca gctgcagcac caccaccacg gcgagcacca gcggcaggcg tttgccacaa
     8281 agccagacca cgaagggcag caccaggtag aactgctcct cgagcgacag gctccagtag
     8341 gggaaggtcg cgccggcctc gaatttcccg taggtgagca tcacccgcag gttgttcacg
     8401 ttgaacaccg cggtgagcgc gccctcgagg ttcgcgcgca gcgaacccca ggcgccggag
     8461 cggtcgaaga acacgcaggc gaccaggatc acggccagcc acagccacgc cgagggaatc
     8521 aggcgccacg cgcggcggat ccagaacgcg agcgtcgtca ccaggcgctc gtgcgtcgat
     8581 tccgctgccg tgatcgcggg aacgaggctg cgcgcgatca cgaatcccga gatcacgaaa
     8641 aagagatcca cgccggacca catcccggtc cagccgaaga aggtcgtgtg cagcggcccg
     8701 ccgacccacg cgaacaggtt catgtgcatg tgctcgatgc acacgaagat gaccgcgatc
     8761 gcgcgcagta cctcgatgtc gtcgatccgt cccttcatct cgtcacgctc cgcaattgtc
     8821 cggccgcgcc gccgcggcgc cggcgcgcag cagcctcaaa cggcgctgca caggtagatg
     8881 ccgcccatga tcagcacgac gccggccatg tacggcaggt cgacgcgctc gcccaggaac
     8941 agcacgctca gcaacggcac caccacgaag gacagcgcca tgaacgggta gaccagcgtg
     9001 agctcgacct gcctgagcac gtagatccac agcgccatcc cgatcacgta gatcagcagc
     9061 gccgcgccga tcagtcccca cgcacgcagc agcggcgcgg cgtcggccgc ggatgccgag
     9121 gcgcccgcct tcatcagtat ctgcgcgacc gcgttcagca ggatcgtgag tatcgcgagc
     9181 gcgtagacct tgttcgtcat catctggccc ccggggcgaa tgaaacagct tcagccgtgc
     9241 agatacgcgc gggcgcgatc gcggcgcggc tgcgcgcagg caaagtgctg gaaaagggaa
     9301 atgccgcgcg acaggccgag caccttcatc gagacctcgc cgaagaaata acgcagcacc
     9361 gcctcgatct cgcgcgccgt gttcacgtgc tcatggcgca tgatcctgcc gtaatcgagg
     9421 cgatgacgca gccagaactc caggccggtg ctcagtctcc agctcaggta ccacacccag
     9481 gtgccctcgc tgggaatcga tgcgcggaac gtgccgccct cggcgagcag caggcacgag
     9541 cgcgcgacca cctccggcag atcgcacagg tgctcgagca ccgcgctcga ggtgatccgg
     9601 tcatagcgcg cctcggcggg cacctcgcgg atgtcggcat ggacgtggcg cacgcgtgcg
     9661 agagcgggcg agtcccggaa cagctcggca aagggctcga cgatgtcgta ggggccgcac
     9721 gccggctcga agccgagctg gttcagcgtg cccgcgccga gctccagcgt gctgacaccg
     9781 ctcgcgccgg ggcgcaggtc ggcagccaag tgccggtgca gccaggcctc cacggcctgg
     9841 gcggcactgg ccgcggccga gccgccctcg cggttctcgc gataccagct cacgtagatg
     9901 cgcgcgatct cctcgggcaa tgcggggcgc gtcttgggga actgctcgaa catcaggccg
     9961 ttgcctcctg cacattgctg cgccgtaccc gcgcgcgcag cgcgatgagc gcgaacgcga
    10021 gcaccgccgc caggctgatg cacaccccga tgcgaaacga cagcggcgcg tagcgcaggc
    10081 gcacgtgatt gaggcccgca tccaggcgca ccgccttgcc cgccagctgt gccgggtaca
    10141 cggtgcggtc ctcgccgttc acgcgcgcgc gccagccggg ataccaggaa tcggcaacga
    10201 acagccagcc cggcgcatcg gttcgcacct cgatgcgcat atcgtcggcc gtcgccgcgc
    10261 gcacctcgat gcgcgccgtc tccggcgccg gcgcggcgtc acaggcactg cgcggatcat
    10321 cgggactggg caggccctcg atcaccagcg gcaggttcgc gcagggttcg gcagcccgca
    10381 accgcgccag cgccgcgtcg gcggattcca cgaacaccgc gtcgcgatag aactgccagc
    10441 gcccgcgcgc ggccggattc tcgctgagcg gcgccagcgc ctcgcccgag cgcagcgccg
    10501 catccgcggc gggcgcgaat ccgccgagtg tcaggtaacg cacgccgagg atgtcggtga
    10561 tgcgtgtccc gggcgcgcgc ggcgtgccct gcagcatttc ctcgcgcacc aggtcggtcg
    10621 cgagcttcga gcgcctgagc tgcagcgcca tcgcgccgtt gatcgccggc acgtcccaca
    10681 gcatgttcgt catcggcgac aacgcggcga gcgcctgcgg tatgcgcagc ggcgcctcgc
    10741 gcgagcgcgc gtgcgcaaag ccgacgatga ccgtattgat cgacaggtcc gcggtgttgg
    10801 ccgcgcaagc gccggcgcgc cagcaggtct gttgcagcac gcccgggcgc gcgacgaggt
    10861 cggtaggcgc gaagtagatt gcctgcgcgc gcagcacccc gatctcgacg gcgagcgcga
    10921 tcagcagcgc cgccggcagc aaggcctcgc gccgcgccag gatcagcgcc acggcgcagc
    10981 cagcgcacag cgcaaggctc gcgtagtgcc accagtgcac ctgtgccgtg tgcaacgcca
    11041 gcgcggcgcc gagtacgggc agcgcgctgc ccagcaacag cgcgacacgg cggcgcgccg
    11101 gccgcccggc atcggcgagc gcctcgacac ccagcgccgc gaccactccc gcgccgacga
    11161 tgccgatata gaaatacggg gtcatcaggc ggaagaaacg catccccggc accaggccat
    11221 ggcggtggat gaactcgaag cccggcgcct gcgcgccgaa gccgagatag cacagcgaca
    11281 gcgtcgccag caccacgccc ggtgcgcgcg cgccgcgcgc cgagaacacg cccgccacga
    11341 acagcgccga gacggccatg ctgccgagca gggtgaaatt cggcgtcagc tcctcgggca
    11401 ccatgtccgt gaacaggaag ccgcgaaaga acgtttgcgg ctggaagcgc acgtaatcga
    11461 cgcccgcggc ccgatgggat tcgccggcca gctcgagcat cggcagcagc tgcgccgcgc
    11521 acagcccgag cgtcagcacc acggccacca gtgccggccc gagcagggca cggacccgcg
    11581 ccacgaattc gccgcgccgc gcccagccga acgcgacggg cagcagcgac aggaacagga
    11641 acaccacgat cccctgcacc acgtgcggat agcccgccag cacgagatag ctcatcgcca
    11701 gcgccagcca caggccgccg ggcacgtcgg cacgccgcaa caggcgctcg aaggcgagca
    11761 tcacccaggg cacggccatc gcgacgttgg agatcgtcag gttgccctgc agcgtcagcc
    11821 acatcgtgga gccgatcgtc gccgcggcgc cgaacagcgc gctcaccggg cggcagccga
    11881 gctcgcggca cagcaggtac acgccgaggc cgccgaggaa ggtgcacagc gcgtggtaga
    11941 cgtcgacgcc ggtgagcggc tcgaacagcg cggcgaccag caggttcagc ggattcagga
    12001 agccgccctg gctttcggca aagaccgggt gcccgacata cacgagatcc gtccacagca
    12061 ggctgtagtc gccgtacagc acctcgcgca ggtagtgcag catcgggatg ccgtggaaga
    12121 gcgagtcacc gatgaggatc gcgcgcgggc cgaacagcgc cggcgcgaac agcgccagcg
    12181 aggccgccag gataagcgcc gcggccgcca ggtcgggacc gcgctgcgtc gtggccacgc
    12241 gcgccgcggt cacggattgc gcctgcccga ctggcgcagc accgagcggt gcagcaccct
    12301 gcgcggcacg ttgccgatgt cgacggtgag gaacaggatc agcaggttga ggctcacgat
    12361 cagctgcagc gcgaccagca tcacggtgcc ggcggtcgcc gggtggccgg tcgcgtagct
    12421 gcggtaccat tccagcgcgc cgaagatgcc gccgctgccg agcagcagca ggccgacgac
    12481 caggctcacg gacccgaggt tgaagtcgcg caggaaatgg ttgtagaaga cacgcttgaa
    12541 caggcggctg gcgtacttca gcggaaagcc gagcgcggtg gcgccgatgc gcaggctgct
    12601 ctgctcgtcg gcgtagtgcg cccgcacggg catgtcctgc accaccgcgc gcagcgtgcc
    12661 cagccggaac agcaggtcgc tctcgaagaa atagcgcggc gccagcttgt ccagcggcag
    12721 catgtgaagc acttcggcgt gtatcgcgat gaagccgttg gtcgggtcca tcacgtccca
    12781 gtagccgctg accgccttgt tcacgaacga cagcacggca ttgccgaaca ggcgcagcgc
    12841 cggcatctgc gccacgtcct cgaggaagaa gaagcggttg cccttggtga cgtcggcatt
    12901 gcccgcgatg atgggctgga ccaggtaggg aatcagctcc ggatcgtact gggcatcgcc
    12961 gtcgagcttc accacgatgt cggcgccgcc ctccagtgcc gcgcgcacgc cactgatcac
    13021 cgcagcgccc accccctggt tttgcgcgtg gacgatcaac cgcacccgcg ggtcgttcac
    13081 ctgctggcgc accagcgcgc cgctgtgctc cggacaggcg tcgtcgacca cgatgatgcg
    13141 cgtgaccagc ggcgggatgc gcgcgatcag cggcacgatc tgccgcgcga cccggtaggc
    13201 gggaatgacg acggcgatgg acgtcatatg gctggcctca cacctcgacc cacgcctcgc
    13261 gtcgcagcag cggctgcacg cgcccgtact gccagcgcgc gaccgggtgg ccaaagcact
    13321 gttcggccgg gatctccggc agcagctcgg cggcgatgtc gttgcgcacg aacaccacgt
    13381 tgaagccgta gcggttggcg cccaccagcc ggtagccctt ctcgcgcccg agcttgacca
    13441 tcgcgggcaa cgaggcgccg caataatcga cctcggtctg cagcaccgga tcgaccagct
    13501 tgcgcacgca gaactcggga tcgtagggca ccgtgaccgc ggcatcggcc atccagccgg
    13561 cctggatctc gatgatcacc acgcgcggat tcgctgccgt gatcgcccgc cacagccagt
    13621 agtcgttgcc atcgacatcg atcgacagca ggtcgatctc gcgatcgaat ccgtagcgcg
    13681 cgatcacctc gttgacgttc tccgccgtga gccatgcctg gcagatcgcc ggagggatgc
    13741 gcatcgtgtc gggatgtcgc cagtagaagt cgttggcgat gcggatgttc tcctcgctgc
    13801 cgtcgcacag cagcgcgtgc cagccgtggt tgatgatcag gttggcggcg ttgcactcgc
    13861 gcccgttgcc ggcgcacatc tcgaccacgt acctgctggt ggtgccgatc acgctgaaca
    13921 ggtagtgcag gatgccgtcc tcgccgttct gcgagtggtt gcggaactcg acgctcccga
    13981 agtcgtgcac cacaccggcg gcggcatcgc ggcggtactg catcgccagc aattgttgcg
    14041 tgccgcggtc gacatagttg ggctgcgccg cctgctcctg cgcggcaacg aggcgcgcga
    14101 ggccggactc cagcgccgcg aggcgcgctt cgatggcggc aaccgcactg tcgccgcccg
    14161 gtgccctgtc ctggatccac ttgcccatgt cgctatgtcc cctgcccggc tgccgcggca
    14221 accggtgtcc cgtgttattt gcgccgcgcc acgatcacgc ggctcttcgg catcagcccg
    14281 ttcagcgcgc ggcgcaagcc ctccgtttcc ggcagctcca gcaggcgcgt ccaggtctcc
    14341 gtccagctct gcaacagcgg atgatacggc ggacggatgc ggtccatcgc cgtcgcgtcg
    14401 tcgccggcct cccgctggct cgcccagaag aagttcatcc acaggaacca gtagaaaccc
    14461 cactggccgc ggccctcgac ctgcagcccg cacgattcgg ccagctcctc cagttcgcgc
    14521 ggctcgatga tgcgcacgtg attgggtgcg gcgaaatacg aggcgtgcgc aaacggctgc
    14581 tggaagcgct ccgaggccgc atccggcacg ctgatcaggt acttcgcgcc cggtgcgccg
    14641 acgcgcacca tctcgcgcag cacggccgcg ggatcctcca cgtgctccag aacctcggtg
    14701 gcgatgatac ggctcatgca cccgtccggc agcggcaggc ggtcgccgct ggccaccagc
    14761 ccggcatggc gcgtgaggcc gcgctcgctc aggcgatccg acagggagcg caccttcgcc
    14821 gggtcgaggt cgcagaatgt cgtgcgcgcg ccctgctgtg ccgcgaacat cacggcaaga
    14881 ccgtcaccgc acccgacatc cagcagctcg tcctgcgggc ccagcgcgaa gccggtgaac
    14941 agctcgccgg tctcggcatt caaccagtcg ctcatcacca tgtcgacgag gccgcaatgg
    15001 cgcgggtcgc gccgcgggtc ggcatcgtcg acggcgtggc gttcgccggc gtcaccatca
    15061 ggcggcgccg gcgcacgacg gcgcagcagt tgcctcagct tcgcgcgcat ggggcatttc
    15121 ccgtcgccgc aagagcggcg gcgcgcgcat cgcgccttgc gtagccgatc gcggcggcat
    15181 gcgccgcgaa gaagcgcgcc aggtgctcgc gcgccacgtc ctgcgaacaa tgctgctgca
    15241 ggcgccggct cgccgcctcg gccatgcgcg cataggcatc cgggtcggcg ccgcgcaatt
    15301 cgcggctcgt tgcgtaggcg cgccgcaacc cttcccactc gggacgaaag cgccgggtac
    15361 gcaggatcag gcccatgtcc tgcggccagt gatacagctc ctcgtgcgca cgcacgggga
    15421 agccgcactc gccatcgagg tagtcgagca tcgcggtgtg cgccggcgcg atggccggca
    15481 caccggccga catgtactcc atgagcggca ggcactggcc ttcgccgagc gaggagttga
    15541 ccgcgaaatc ggtggcgcag atgagcctgg cgtactgcgc gtcatcgaga tgcgcgtgca
    15601 gcagcacgat gcgcgccggg gtgcgcggga gcttgcgcag cagcgccagc acctcctcga
    15661 gcgccttgtc gcgatcgtga tgcaccagct tcagaaccag cgtgcagtcc gcgacatccc
    15721 ggaaggccca gcagaacgcg ctgacgaggt cctcccagtt cttgcgtccg tccagcgggc
    15781 agaacacggc cgtatacacc acgccatcga ggcgcagctg ctggcgccgg cgcgggtgcg
    15841 cggcgatcag cgcctgtggc ggctcggtga aatcgcatgc gcggctgtcc atgagggtcc
    15901 cgtccagttc gacggaccgg tcggcgccgc tggtctcgcg tcgccgcgcg gcgagcggcg
    15961 cgaagcggtc ccagacgggc gcgggcaccg acacgaccgg atagtccggc cgtatcgcgc
    16021 gccgcaccac ccgtgccgtg aattgcgagt gcgtgatggc gctgcccagc atcgccagca
    16081 cccgcaacca gtcggtctcg cgcctgtcgc cccagggctc gtcgggaagg ctgtcgtact
    16141 cccaggcgaa caccggcacg accgggcact cgaggcgcgt gggcacccgg tgcggcggcg
    16201 cgaaacacag cagcacgcag tattcgccgc tggcggcgca gtcgcggtac caggcgtccg
    16261 cctcgcgcag cgggtccgcg atctcgacca ccctgcccag cgcctgcagc acgggcaggt
    16321 aggcctgcat cacgaagtag tagctgtagt cggcgcgtcc gacctcgcct tcgatcgagt
    16381 ccgcggtgaa cggcacgtgg accaggaaga tcatgcgtcg gcacccgggc gcaaccggcc
    16441 ggcctcgcgc tgcgcgccgg cggccggcgc cacgccgagg aaagccccga tgctgtcggt
    16501 cacgcgctcg accgtgcagt agcgctgcat cgccgcagcc gccgccacgc ccatctgccg
    16561 gtacgcctgc gggtcctcgc ggtatacgcc gagactgtga cggaaagcgg caaccgtcga
    16621 ctcccagctc acccggtact gcagggtgcg cagcagcgcg cgttcgtcat ggggccagat
    16681 gcacggctcg gtggtggcct cgatcgcgat cgaattggca tcgtcgaggt agtcgagcag
    16741 cgaggtatgg cgcggcgcca gcgccggctt gccgcagctc atgaactcca tcagcgggat
    16801 gcagagccct tcggccttgg acacgttgac gtagaaatcc gtgcgcccga tcagctgccc
    16861 gtaatcctgt tcgtcgagga aaccgtggac cacgaccacc cggcacgcaa acgccggcag
    16921 gcgctgcatc aggctgtgca gctcgaagat gtagctgcgc tgcaactgct gcgtcatctt
    16981 cagcagcagc gtgacatcgt cgcgcccggc gaacgcggtg cagaaggcag agaccagcat
    17041 gtcccagttc ttgcggtcgt cgcggggatt caggacgctg gtgtagacca caccgtccag
    17101 cgtgagcgcc tgccgcacgt gcgccgccac cgcgcgcgcg ggtgcggggg gcaggggcat
    17161 ggcatcgagt atgtcgagct cgtcgatcgc caggcggcac aagccgatgc cgagcgtgcg
    17221 tgcgtcctcg ggctcggatg ccggcagctt gcggaccccg gtgaacccca ccacctgcgc
    17281 cggctcggtc agatcgaatt cgagcgcgat cacctcggcc tcggccttcg gcactatcgt
    17341 gcgggtctgc tcgccgaggt agaggctgag cggcgccccg gcattctgcg catagccgac
    17401 catttccacc cgcatggcta ttcgcccgtt caccggccgg gggagcatca gccacggcgc
    17461 tgtcgaagcc gaccaggcac cccacggctc gggggcccag aactcctgca gcagcgcgct
    17521 ctcggggcag ccggcgcgca attcgagcct gatcgtgccg tccgacggcg cttcgacggc
    17581 gggcagcagc ctgagcccgg tgatgccgaa gccgatgctg cgctcctcgg cggcccccgc
    17641 cagcgggcgg ggatcgatgc ccatgatgtg cagcatgcac gcgggccggc cgagccggaa
    17701 gcgcagcgta tgcaattcct cgcccccgcc gatgcacagc ggctggaacg catcgcccag
    17761 cgccgcgacc aggggccggc cctggttcct gccgtaggcc cgcacgtcga gctcgatggc
    17821 cacctcgccg tggagcagcc acggcagcgc gatcgcgatc tcggcgttgc gcgaccacgc
    17881 gccccagggc tcgggccggt aaaaacccag caacgcaccc gtgtctggcg acagcaggcg
    17941 aaaatccagc gcgtgcgcct gtccgtccca caactcgagc gcatacgcgg cgtaggggcg
    18001 gttgcatcgc agccggtccg ggcccctctc gaagtcacgc gtgtcgagca cggcgccgtc
    18061 cacgcaaatc ccgggctggc cgtcctcgcg cccgctggcg aggcgctcgc gcacgccggc
    18121 catgcgctcc cacagcggca ccggcaccgc cagcaccgga aactgcgcgc ccatcgcccg
    18181 ctgcaacgcc gcgaccgcgt gctccgagag cgtgatcgcg cgcgcacagc cggccagcgc
    18241 cacgcgccag tcgttgcgcg gattgccacc ccacggctcg tcgggaatgc gctcgaactc
    18301 ccacgcgatc accggcacga cggggcaacg ccgcacatcg ggcaggttct gcggcgccgt
    18361 gaacgcgatg agcacgcatt gctgcccttg cgcactgaag cggtcgaaca gcgcatcggc
    18421 ctcggcggcg ggatcctcga cgacggtcac cgcgcccagg cgctcgaagg cgacgcgaaa
    18481 ctccttcagc aggaagtaat agctgtactc gggcgcgccc agcgagcgcc cgatgttgcc
    18541 ggcattgatc cgcgaataga gcaggaagtg catcggccgg cccacgtctc agctacccgt
    18601 ggcgtcggcg acacggccgg cagcgtccgg ggcgtccagg ctggccaccg gcgccagcca
    18661 gttgcagccc acgaagcgcg gatgcgcgac gttgatcacg tagaagatca gggcgcggtc
    18721 cctccattcg taggtgcggt cgacgtgcgc gtcggaacgg gtcagcgcgg tggtgacgga
    18781 ataatgcccc tcgcccaggt tcatcacgaa gcgcaggcgg aacacgtgct ccgagccggc
    18841 ggcaaggttc tcgatcggga tcgacagccg gtggctgttg atgccgaaca tctcgtgccc
    18901 gaggcggtcc ttgatcatga gcccgaccac cagcgtgggc aggtccacgt gcacgcgaaa
    18961 gcgcacgctc atcacgactt cctcgccgac cttcgcggca tcggtggtcg cgccgcccgc
    19021 attctgcagc gtcaccgaca cgatcgtggc ttcgccggtg cccgagaagg tctgcaccac
    19081 gccctgcgca ttcagctcct ggcggatgtg cgcgaaggtg cggtccgcca tcagggcgtg
    19141 gtagaaatcc atcacgatct cgggctcgcc ctcgcgcagc agccgcccct cgtgcagcag
    19201 gatcgcgcgg tcgcacagcg cctgtatcga caggcggtcg tgcgagacga tcagcagcgt
    19261 ggtgccctgc tcgcggaact cgcggatgcg cgaaaagctc ttgtgctgga agtaggcatc
    19321 acccaccgag agcgcctcat cgacgatcag cagctccggg cgccgggccg tggccaggct
    19381 gaaggcgagg cgcatcgcca tgccgctcga gtaggtgcgc aggggctcgt cgaaatacgc
    19441 gccgatctcg gcgaagtcct cgatcgccgg catcagctcc tcgagctcgg cgcgctccag
    19501 cccgagcagc tgcccgttca tcatcacgtt ctggcgcccg gtgaactcgc cgtgaaagcc
    19561 catgccgagt tcgagcagcg ccgcgacgcg cccgtgcagg cgcacctcgc cctcggtggc
    19621 gcgcgtggtg cccgtgatga tccgcagcag cgtgctcttg ccggcgccgt tgaagccgac
    19681 gatgccgacg gcctcgcccg gcgacaccgt gaagctgacg tcgcgcaacg cccagctctg
    19741 ctggtggcgc ggcccgccga agggcagcag ccattcggcc agccgcgacc agcggttcgg
    19801 atagtggcgg taggccttgc ccagcccgat gacctcgacc gtatggctca tagctcgtcc
    19861 acgatctcgt cggcgcgccg gcgaaacagc gcgagcgcga ccaccagggc gagcgtcgcg
    19921 cacagcgcgg gcgcgaccag cgcggaccac tgcggccagc cctgtcccag cgcgatcgcc
    19981 tgctgcgaca gcatcagcgg cgtcagcggg ttggccagca cgatgggccg gatggcctcg
    20041 ggcagtatcg acagcggata cacgatcggg gtgaaccaga accagaactg cagcagtatg
    20101 ccgaagaact gccccgcatc gcgaaagaac acgttgagca cgccggcgac gatacccagg
    20161 ccgagcgcga acacgcagag gatcgccagc accggcagca tcgccagcag gtaggcgccg
    20221 gggaaattcc ccgtgaccag cagcagcagc aggaacaggc cgtagatgat cgcgaaattc
    20281 atcccggcat tgagcagcac gatcagcggc aggcagatgc gcgggaaatt catcttcttc
    20341 agcaggttcg cgtgatcgag gaacaccgtc atgccgcgct ggatgatctc ggagaacagc
    20401 ccccagctga ggatgcccgc gcacaggaag atgccgtagg ccagcgtgcc ggtttcgccc
    20461 gggagccgcg cctgcatcac ctgggagaag atgagcatgt acaccgcgac catcgcgagc
    20521 ggattgatca ccgcccaggc cgaacccagc agcgaattgc cgtaacgcgc ggcgaactcg
    20581 cgccgcacgc tgctgacgat gaacccccgg gagcgccacg cagcgcgaat cagctggatc
    20641 agcacgcagg cgccctgatg aaaaagtggg gcgcattata gccgcatcgg ctcatcacgc
    20701 cgatgccgtc cagcgctgcg cggcggccat gccggcgagg tgcaacagct cgatgtcgcc
    20761 gattcgcctg gccgtcgtca tcgcctgact gcccatggca ttcatccggt caacgccttg
    20821 aaccacaccg cgtagggctg gtacagccac cacttcagcg gcacgcgccc gtagtggcgg
    20881 cgcaccacgg cgatggcctc gcggtaatgg cgcgcgcgat tgcggcgggt cttggtcccg
    20941 gcgtgctcgc ggttcacgcc ggtcacggcg tccacgaaac agggcgcacc gcagcggcga
    21001 tagagccgcc accacaggtc gtagtccatc accatgtgca ggctctcgtc gagtccgccg
    21061 accgcctccc aggccgagcg gcggatcagc gtggcgggct gcgacacgat gcagcgcagc
    21121 gccaggcggc gctcgtcgaa gggctccacc cacaccggat cgcgccggcc cgtggcctcg
    21181 accaggttcc atgcccgcgc ataggccatc ggcgcggcgg gggcggcatc gagggcggcg
    21241 atcagcgtgg cgagggcccc gggcaacagc aggtcgtcgc tgttcagcca gcacacgtag
    21301 ggcgccgagc cgcgcgcgat gcactcgttg atggccgccg cctggccgcg gtcggcgtgg
    21361 ctgcgccagc cggcgagccg cggcgcaaag cgctcgatga ccgcgaccga atcgtcgccc
    21421 gagccgccgt cggccacgaa gacctcgacc gcaacgccct ggtcgagcgc cgattccagc
    21481 gccgcgccca gatagcgccc ctggttgaac gacggcaccg cgatcgtgac cgcggcgtac
    21541 acgctggcca gcacgcacca cacgaggg
//
LOCUS       DAORUQ010000032        21518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2022_length_21518_cov_6.598705, whole genome shotgun sequence.
ACCESSION   DAORUQ010000032 DAORUQ010000000
VERSION     DAORUQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_21518_cov_6.598705"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PLW13_04760"
     CDS             complement(<1..1393)
                     /locus_tag="PLW13_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY69712.1"
                     /translation="MSDPAQQSRLKDLIAKAKEQNYLTYAEVNDHLPEDISDPDQVEE
                     IIQMINDLGIQVFEEAPDAEALLLTSGDTQADEIADEAVAALTAVESEAGRTTDPVRM
                     YMREMGTVELLTREGEIQIAKRIEEGIRETMAAISYFPGTIEQVLKEYDLVATEERRL
                     SDILTGYLEPADEVPAATPAEPAGKPGAKKAAAATDDEEEEESEDGSDTDEVESGPDP
                     ELARQRFDELRKQLAKAVRTIKKHPRDSIQAQKELEKTAELFKFFKLSPRQFDPLTER
                     VRTTANQVREHEKVIMNLCVNYARMPKQVFLKEFPGNEANDKWIEKLARRKQPYATKL
                     HEVRDQVAQVQQKIAEIEGAHGVSVAELKEINRRMSIGEARARRAKKEMVEANLRLVI
                     SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQ
                     ARTIRIPVHMIETI"
     gene            complement(1532..3334)
                     /gene="dnaG"
                     /locus_tag="PLW13_04765"
     CDS             complement(1532..3334)
                     /gene="dnaG"
                     /locus_tag="PLW13_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQY69713.1"
                     /translation="MSGRIPQQFIDDLLERVDIVDVIDSRVNLRKSGKNYSALCPFHD
                     EKSPSFSVNPDKQFYYCFGCGAGGNAIGFLMEYERLDFPAAVEKLAGVAGVEVPREGG
                     AAAASPRQAELASRLEAAAKWFQAQLRTHAQAAQARQYLQGRGLSEETIERFGIGFAP
                     PGWDGLLNALGRSEADRTLLLDAGLLIARDDGGLYDRFRHRIMFPIRDNRGRAVAFGG
                     RVLTDEKPKYLNSPETAIFHKSQELYGLFEARRTVRDLDRVLVVEGYMDVVMLAQHGI
                     NNATGTLGTSLTSNHLQRLYRYTPSVIFCFDGDEAGRKAAARALEIALPAMQDGRQAS
                     FLFLPEGEDPDSLVQRIGADAFRKLLSTATPLSEYLFEHYGQAIDLEHLDGRARLSNL
                     LLPAINRIPGAVFRELMRTALAERVGMPVNRLPQAPGQAPVTASPPTDSAAPVRPQAT
                     RSPSPTPAATAQRRVQGSTRHAIRLLLQHPELAARTPDFERLETLEDPDLPLLLQLAK
                     QLKQTPHTSLGAMLGYWYGTESGELLTRLAAKDPVPGADPEREFDDVMEHLAQRASRK
                     SATEQLRELERISFAELSPAQKQQYLELLQLRKK"
     gene            complement(3414..3866)
                     /locus_tag="PLW13_04770"
     CDS             complement(3414..3866)
                     /locus_tag="PLW13_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003137358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQY69714.1"
                     /translation="MSDATLKTRITEAMKAAMRAQEKARLGAIRLIQAEIQRVEVDER
                     IAVDDARVLAILDKMIKQRRDSIQQFESAGRLDLVEIEAGELAVIQEFLPRALGEPEL
                     ELLIDGAMHASGAVAMKDMGKVMAILKPQVQGRADMGLVSQRIKARLT"
     gene            complement(3889..4104)
                     /gene="rpsU"
                     /locus_tag="PLW13_04775"
     CDS             complement(3889..4104)
                     /gene="rpsU"
                     /locus_tag="PLW13_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQY69715.1"
                     /translation="MPSIKVKDNEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTWI
                     RKRKAAAAVKRHLKKVSRESRRQQRLY"
     gene            4276..5307
                     /gene="tsaD"
                     /locus_tag="PLW13_04780"
     CDS             4276..5307
                     /gene="tsaD"
                     /locus_tag="PLW13_04780"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQY69716.1"
                     /translation="MRVLGIETSCDETGVAVFDSDRGLLAHCLHSQVAMHAEYGGVVP
                     ELASRDHIKKALPLIRRVLQEAACPVETIDGVAYTAGPGLLGALLVGGCVARSLAFAW
                     GVPAVGVHHMEAHLLAPMLEPERPVFPFVALLVSGGHTQLVLVEGIGAYRILGESLDD
                     AAGEAFDKVAKMLGLGYPGGPQLAALAADGVPGRIDFPRPMTNRPGLDFSFSGLKTAA
                     ANALRGLQQAGGVIAPQCKADIAHGFQQAVIDTLLIKCRRALQSTGLTQLVLAGGVSA
                     NRELRERLRDEAQARGWRVFYPRAEFCTDNGAMVAFAGCQRLLAGQHDDLAVRAQARW
                     PIDTLEPVA"
     gene            5339..5701
                     /gene="folB"
                     /locus_tag="PLW13_04785"
     CDS             5339..5701
                     /gene="folB"
                     /locus_tag="PLW13_04785"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQY69717.1"
                     /translation="MDVVYIRGLRVDTVIGVYPWERTVRQVLEFDLEMARDTAAAARS
                     DAIADALDYHAVAERVRALAAVSSCQLVETLAETVATALMAEFAVPWLRIKLDKPGAV
                     PGARGVGVSIERGVRRDQ"
     gene            complement(5695..6447)
                     /locus_tag="PLW13_04790"
     CDS             complement(5695..6447)
                     /locus_tag="PLW13_04790"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HQY69718.1"
                     /translation="MGNSSSGRVALVTGAAHRIGAAIARELHRAGCRVLIHYRSSAAG
                     AQQLLRELNAVRPDSCAAVQADLCRANDVRELAAQAWALAPGLDLLVNNASAFYATPL
                     GNVTDAQWNELFDANLKGAFFLSQALAPALARRQGSIVNIVDIHADIPLPQHTVYAMA
                     KAGLAMMTRALAVELGPSVRVNGIAPGAILWPEQPNAVLGAAHADALLAQTALKRMGT
                     PEDIAGAARFLGLEAPYVTGQILAVDGGRNLY"
     gene            complement(6437..7669)
                     /locus_tag="PLW13_04795"
     CDS             complement(6437..7669)
                     /locus_tag="PLW13_04795"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HQY69719.1"
                     /translation="MQIYLVGGAVRDRMLDIPPCERDWVVVGASPEQMLALGFRQIGR
                     DFPVFLHPETSEEYALARRERKSGHGYAGFTCETGSQVSLEDDLARRDLSINAMAEDR
                     DGHLIDPYGGAQDVRDGVLRHVSDAFVEDPLRVLRVARFMARFASRGFRVHPATMTLM
                     RDMAASGELAHLVAERVWTETRRALGEASPQEYFRTLRECGALGAIFPEIDALFGVPQ
                     PARHHPEIDTGEHVLLCLRLAAQLRLTEPARFAVLVHDLGKALTPRERWPSHVGHEKL
                     GAVAVRALCERLRVPVKYRELALVVCLHHTRCHRVLEMRAPAIVSLLEDVDALRNGER
                     FEEFLAACEADARGRTGLEQRPYAQAEKLRRARAAAATVTAASALARGLHGPQIAVEM
                     HRLRCIEVARLEGEDHGE"
     gene            complement(7809..8615)
                     /locus_tag="PLW13_04800"
     CDS             complement(7809..8615)
                     /locus_tag="PLW13_04800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HQY69720.1"
                     /translation="MATYAIGDVQGCHQPLLRLLDTLRFDPTADRLWFAGDLVNRGPE
                     SLQVLRFVRGLGERAVMVLGNHDLHLLAVSEGVRKALRKDSLGAILEAPDREELLGWL
                     RCQPLAYRGSDFTHVMVHAGIPPQWSVEDTLARSREVEATLQGPLYRDFLHHMYGNFP
                     DTWDERLEGFERLRLITNYLTRMRFCAADGKLDLDNKSPPADANPGFAPWYAHALRRS
                     AGVPVVFGHWAALEGRAERADVHALDTGCVWGGRLRAMRLEDGALFHCNC"
     gene            complement(8615..9421)
                     /gene="rsmA"
                     /locus_tag="PLW13_04805"
     CDS             complement(8615..9421)
                     /gene="rsmA"
                     /locus_tag="PLW13_04805"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQY69721.1"
                     /translation="MAGGPAGHRPRKRFGQNFLTDRHVIDRIMQAIDARAEDTIAEIG
                     PGHGALTEPLLRSGARLHVVELDRDLVARWRLHESERFSVHAADALDFDFRALAPPGG
                     GLRVVGNLPYNISTPLLFHLLEQGDCVRDMHFMLQREVVERLGAEPGSADYGRLSVMV
                     QYRCAVQPLFEVPPGAFHPPPRVVSAIVRLLPQSHPHGRAHDYRMLEQVVRQAFGQRR
                     KTLRNAVAGLLDAATLERLGIDPGRRPETLTVGEFVALSNAVAPQPGAGA"
     gene            complement(9421..10407)
                     /gene="pdxA"
                     /locus_tag="PLW13_04810"
     CDS             complement(9421..10407)
                     /gene="pdxA"
                     /locus_tag="PLW13_04810"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQY69722.1"
                     /translation="MLALTPGEPAGIGPDLVVTLAQRGFDASVIAIADPQLLLDRARA
                     LGLPLDILPPDPLPAPPRTLRVLPVALGRPVVPGTLDKANAPYVLATLQAAVSACMKR
                     RVAALVTGPVHKGIINDAGVPFSGHTEFLAAATGTPRVVMMLATPGLRVALATTHLPL
                     RDVSDHITAQSLEQVLRILHRDLRRHFGIRDPRVLVAGLNPHAGEGGHLGREEIDVII
                     PCLQRLRAEGMTLIGPLPADTLFTPHHLEGADAVLAMYHDQGLPVLKYKGFGQAVNIT
                     LGLPIIRTSVDHGTALDLAGTGKADTGSLQFALQTAHDMAARSAALRAAERG"
     gene            complement(10427..11737)
                     /locus_tag="PLW13_04815"
     CDS             complement(10427..11737)
                     /locus_tag="PLW13_04815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY69723.1"
                     /translation="MRIESTGAGSRWRAAAAIVLCCWGLAGGALAQTRQLEGVVAIVD
                     DDIILASEYQDRMRQVTENLARQKIEMPPQEVLARQVLDRLILERIQLRMGDRAGVRI
                     SDEQLNEALTNMARQSGASLEQFRAQIEADGGSYAAVREQVRQEMILSRVQQGNVRNR
                     VQVTEQEVDDYLASEEGQKRTAAVYHIGHLLLPLATDATMEQERAAMAYMEGLRSRLA
                     SGTAFERFMRAPDKTPYAFTGGDLGWRLAGDLPSVFLETVPALETGAISAPVRSASGL
                     HLVKLFEKRGGSGQMTQQTRVRHILIKPSEVRSDAQAQQLAAQLRARVLAGEDFAKLA
                     REFSEDIGSAREGGDLGWTSAGQMVPEFEQAMNQTGAGEISAPVRSSFGWHVLQVEER
                     RTQDLSDEMKRNQARNILFGQKYDDELNTWLQKIRDEAFVEIKI"
     gene            complement(11727..14369)
                     /locus_tag="PLW13_04820"
     CDS             complement(11727..14369)
                     /locus_tag="PLW13_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HQY69724.1"
                     /translation="MQSKPVIFLSRVIAGLASAAVLHAAAADNGQWQCRGAETGGWDC
                     SAVAPTIASGAVRSTPSAAPNVAADATPDVTPDAALDAVPAAAPDAAPAGSVAGLADA
                     DLLAETMDWVTLRRLSPEQAAAIGAQCRGAYIEPPLEPPAEGGAQIGTVHASSAESEL
                     LQNPEIAKFSGNVTLLQGDRRLRADRATYYREQDRADIEGNVQYREPGLLVRGESASI
                     DTAQNSGELREARFVMHREHARGEAKLVRRNVDRTLEFEETVYTQCEPGHDDWQLAAD
                     SMRVDRETGRGTAQNARLEVKGIPVLYTPYLSFPVDDRRMSGLLWPTFTNSSQNGLDV
                     TTPYYFNLAPNYDATLLPRYMNDRGTMIGGELRYLNRWSSWATSGAILPNDEVADEDR
                     WIASLEHTGAPVERMGTLISYTEVSDEDYLRQLSSTGLEVQRSIFLPQTGQVTYRLDD
                     SWVLAAKAQQYRLLDRALAEPYKIMPRLEMNRPFSGTPFALDYSLTSEFTVFEHKDRA
                     ELTGQRLYLEPQIAFPMQWAAAFVRPALGYQSISYNLDESLTLPGDESPSVGAAMVNL
                     DAGYFLERDTSLFGTAFLQTLEPRAFYLWVDREDHSDLPNFDSSDLTFSFSQLFRTTR
                     FSGRDRIADANQASLSVTSRLIDDENGMERVTASVGQIYYFEDRLVTVCDAQRAQSQP
                     SCRTRVPPSGGWSDAQPADAQSVSSSEIAAEVQVRPTRSVSVTGTTLLDVHREQVNEG
                     GAFVHWTPDTQTIVNAGYRYRREQTSYDIFGNLISETIDQADFSAVLPISDRWRVFTR
                     YQYDFTNDYSLEELAGLEYSSCCWSVRMVYQEGVDWDQGRDYGFYLQFVLRGLGGLGK
                     NIDQLLQDSIFGFGSNLEDNGLAY"
     gene            14556..15569
                     /locus_tag="PLW13_04825"
     CDS             14556..15569
                     /locus_tag="PLW13_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY69725.1"
                     /translation="MDALLQWCSKLVEPVAGRRLGLSAVAGDASFRRYFRLEGCEPTR
                     IAVFAPPDKERNLEFTRIAAMLREAGVCAPEVIAHDSASGFLLLSDFGDTLMLGALDE
                     HSVDRLYARAMDTLLRIQGTATACEGWHLPAYSGALLLQEMQLFPDWYLKGLLGIALG
                     AHDRALLQELFARLVASAQEQPQVFVHRDYHSRNLMLLPGGALGVIDFQDAVTGPLTY
                     DLVSLLRDCYVQWAPARVEAWAASFAARAVAAGSMQPVPAATFRRWFDWMGLQRHIKV
                     LGIFARLWLRDGKPGYLHDLPLVMRYTLDVAAAHPETAPFADWLRDRVLPVARGQDWY
                     RAR"
     gene            15566..16264
                     /locus_tag="PLW13_04830"
     CDS             15566..16264
                     /locus_tag="PLW13_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQY69726.1"
                     /translation="MKAMILAAGLGTRLRPLTETLPKPLLPLAGKPMIVYHVEKLAAA
                     GLTELVVNVSWLGALIEQHLGDGEAFGVAIRYSREPEGPLDTGGGIRRALPLLGEQPF
                     LLVAGDIWSDYPLARLAARALAEGELGRLVLVPNPPQHAGGDFSLDAAGRVGTSAVGR
                     RCTYSGISLLSPRLLDGWSDTVFPLREPLRQAAGRGVLSGEIWTGDWEDVGTPERYAA
                     LNRRLDRRPESPPA"
     gene            complement(16221..17183)
                     /locus_tag="PLW13_04835"
     CDS             complement(16221..17183)
                     /locus_tag="PLW13_04835"
                     /inference="COORDINATES: protein motif:HMM:NF023472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HQY69727.1"
                     /translation="MRRTTRLLQPLLLGLVIAGPPGNAAEPQAPAPAASPVPAAAPAV
                     PAAPAAPEPEPAPAIDRDLDALAVVLAQERRILKADGHEFAVFVTQARGPVARGALVL
                     IPGDGSHPTSAAGLEQIRQSMPAHGWATWLLTLEPPPRIHAEQIGTPGAAPPAATESA
                     PAGAGSATLNARRAGALEQWAVRSQARILAAIASAAAEGRPLVLVAEGSAAVLLTRGM
                     GSAPGSVRAAALLDPVELPGLVLEWPRGLARPVLEVLSPTAPREQGRLRREQAAALQL
                     KHYRQLTLETGSWHPGPADAPLTRRLRGWLTTLEATPGAGPGAG"
     gene            17285..17962
                     /gene="rpe"
                     /locus_tag="PLW13_04840"
     CDS             17285..17962
                     /gene="rpe"
                     /locus_tag="PLW13_04840"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQY69728.1"
                     /translation="MNDFQIAPSILSANFARLGEEVDAVLAAGADIVHFDVMDNHYVP
                     NLTIGPMVCRALRDHGVSAPIDVHLMVTPVDAIAAAFAEAGATYITFHPDASTHVDRT
                     LQLIRDAGCKAGLVFNPAAGLDAARYVMDKLDMILLMSVNPGFGGQKFIPATLGKLRE
                     ARALIDASGFPIRLEIDGGVGTGNIRAIAEAGADTFVAGSAIFGAPDYAAVIAGMRAE
                     LAAARRP"
     gene            17967..18662
                     /locus_tag="PLW13_04845"
     CDS             17967..18662
                     /locus_tag="PLW13_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQY69729.1"
                     /translation="MAHALIRRLLDGRLPALAMFDLDGTLVDSVPDIARALDATLAAR
                     GFAPAGSERVRHWVGRGSRNLVREALAHAVDAAPEHVAEDLLDEALGFYLARYLEDCA
                     GATTLMPGAAALLASLRAAGVALACVTNKPIAITERLLGALLPDAGFAAVLGGDSGAG
                     VKPAPGPLLAAMAKARTLPCAALMIGDSRHDVHAARAAGVRVICVAGGYNHGEDIRRE
                     APDLVVDGLGELL"
     gene            18808..20289
                     /gene="trpE"
                     /locus_tag="PLW13_04850"
     CDS             18808..20289
                     /gene="trpE"
                     /locus_tag="PLW13_04850"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQY69730.1"
                     /translation="MNATRLEQLAREGFTRVPVVRRLLADLETPLGAYLKFAGPFSFL
                     FESVEGGEKWGRYSIIGLPCRRRLVVRGTRITLCDGDAEQDVAHADPLAFVEEFLARE
                     RALPHEDLPMFAGGLVGYFGYDCVRMVEPRLAASAPPDVLGTPDILLMVAEEFLVFDN
                     LAGAIALVVTVDPREADAAAGAEARLDALQQRLEGPAPRLPPVDIDAGRRDSQRVRDV
                     EAAFESEFGRERFEAAVERIREYILAGDVMQVVPSQRMSAPFSASPLNLYRALRNLNP
                     SPYLYFLDLGDFQIVGSSPEILSRLQDGEVTVRPIAGTRRRGATHEEDLALEAELLAD
                     PKEIAEHLMLIDLGRNDVGRVAGIGSVTVSERMIVERYSHVMHIVSNVSGRLREGLSA
                     IDVLRATLPAGTLSGAPKIRAMEIIDELEPTRRGIYGGAVGYLSWHGEMDTAIAIRTA
                     VIKDGRLHVQAGAGIVADSVPALEWEETMNKARAMFRAAAMLS"
     gene            20303..20947
                     /locus_tag="PLW13_04855"
     CDS             20303..20947
                     /locus_tag="PLW13_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HQY69731.1"
                     /translation="MLALSAMDWLAFAGTFAFAVSGALLAARHRLDIIGFLFVANMTG
                     VGGGTVRDLLLDAPVFWVQDATYIVVCSAAALLTYAAASLLDRASQALLWADAIGVAL
                     FAVLGAQKALLAGVGPGVAVVMGIFTACFGGIIRDFTLNEPPIIMSREIYITATLVGA
                     VCYVLLAGLRGAQDIVSVVGGTAVGFTVRALAIVARLSLPSHAGLGHKDGGSAQ"
     gene            complement(20994..>21518)
                     /locus_tag="PLW13_04860"
     CDS             complement(20994..>21518)
                     /locus_tag="PLW13_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQY69732.1"
                     /translation="LQRAIAGERIERDHDAQPASAYREDDKRGTVGAVALDRHGNLAA
                     GTSTGGLTNKRYGRIGDSPVIGAGTWADNRSVAVSATGTGEMFIRTAAAHSVAELVRL
                     VGKTAADAADATLAEVAAIGGDGGLIVLDAKGDYAMRFNTSGMYRGTVGEDGAGWVGI
                     FGGPERRISLPVAP"
BASE COUNT         3063 a         7239 c         7502 g         3714 t
ORIGIN      
        1 tgatggtctc gatcatgtgc accggaatgc ggatcgtgcg cgcctggtcg gcaatggacc
       61 gggtgatcgc ctggcgtatc caccacgtgg cataagtcga gaacttgtag ccccggcggt
      121 attcgaactt gtcgacggcc ttcatcaggc cgatgttgcc ttcctggatc agatcgagga
      181 actgcaggcc gcgattcgtg tacttcttgg caatggatat caccaatcgg aggttcgcct
      241 cgaccatctc cttcttggcg cgtcgggcgc gggcctcgcc gatcgacatg cgccgattga
      301 tctctttcag ttcggcaacc gacacgccgt gcgcgccctc gatttccgcg attttttgct
      361 ggacctgtgc cacctggtcg cgcacttcgt gcagcttcgt agcgtaaggc tgcttgcgac
      421 gcgcaagctt ttcgatccac ttgtcgttgg cttcgttgcc cgggaattcc ttcaggaaca
      481 cctgcttcgg catgcgtgcg tagttgacgc acaggttcat gatcaccttt tcgtgctcac
      541 gaacctggtt ggccgtcgtg cgcacgcgct cggtcagcgg atcgaactgg cgcggagaca
      601 gcttgaaaaa cttgaacagt tccgctgtct tctcgagttc cttctgcgcc tggatactgt
      661 cgcgaggatg cttcttgatg gtgcggacgg ctttcgccag ctgtttgcgc agttcatcga
      721 agcgctgccg ggcgagttcc gggtccggcc cgctttcaac ctcatcggta tcgctgccgt
      781 cctcgctctc ttcttcctct tcgtcgtccg ttgcggccgc agccttcttc gcaccgggtt
      841 tgccggctgg ttcggcgggc gtggcggccg ggacttcgtc ggcaggctcc aggtatccgg
      901 taaggatgtc cgaaaggcgg cgctcttcgg ttgccacgag atcgtattcc ttcagcacct
      961 gctcgatggt gccagggaaa taggagatcg cagccatggt ttcccggatg ccttcctcga
     1021 tccgcttggc aatctggatc tcgccttcac gagtcagaag ttccaccgtg cccatctcgc
     1081 gcatgtacat gcgcaccggg tcagtggtgc gtcccgcttc ggattccacc gccgtgaggg
     1141 cggcaaccgc ctcgtcggcg atttcgtcgg cctgggtgtc gccgctggtg agcagcaacg
     1201 cttcggcgtc gggcgcttcc tcgaagacct gtatcccgag gtcgttgatc atctggatga
     1261 tctcttcgac ctggtccgga tccgaaatgt cttcgggcag gtggtcgttc acctcggcat
     1321 aggtcagata attctgttcc tttgccttgg caatgagatc tttcaggcga gactgctggg
     1381 ctggatccga cattcgtgaa cctgcatgag tgtcaggggg cgagaagata gccgtgcatt
     1441 ataagacaga agtgacgtcg ggccctacga aaaaatacgg ttcgcgccac catgggcgcg
     1501 acgcgtggcc gggcacgccg gtagcgtggg cttacttctt gcgcaactgc agtagctcca
     1561 ggtactgctg cttctgtgcg gggctcaatt cggcaaagga aatgcgctcc agttcccgca
     1621 gttgctccgt ggctgatttc ctgctcgcgc gctgtgccag gtgttccatc acgtcgtcga
     1681 actcccgttc cggatcggcg cccgggaccg gatccttggc cgcgaggcgc gtcaggagtt
     1741 cgccgctctc ggtaccatac cagtacccga gcatggcgcc caggctggtg tgcggggttt
     1801 gcttgagctg tttcgccagc tgcagcagca gcggcaggtc cggatcctcc agcgtctcga
     1861 gccgctcgaa gtcgggggtg cgggcggcca gctcggggtg ctgcagcagc aaccgtatgg
     1921 catgccgtgt cgagccctgg acccggcgct gcgccgttgc tgcaggcgtc ggcgatggcg
     1981 atcgcgtcgc ctgcgggcgc accggcgcgg ccgagtcggt cggcggtgat gccgtgacgg
     2041 gcgcttgacc gggcgcctgc ggaaggcgat tgaccggcat cccgacccgt tcagccaggg
     2101 ccgtacgcat gagctcgcgg aagacggccc cgggaatccg gttgatcgcc ggcagcagca
     2161 ggttgctgag ccgggcacgg ccgtccaggt gctccaggtc gatggcctgc ccgtagtgct
     2221 cgaagaggta ttccgacagc ggcgtggccg tggacagcag cttgcggaac gcgtccgccc
     2281 cgattcgctg caccaggctg tcggggtctt cgccttcggg caggaacagg aagcttgcct
     2341 ggcgtccgtc ctgcattgcc ggtagggcaa tctccagcgc gcgcgcggcc gccttgcgac
     2401 ccgcttcgtc gccgtcgaag cagaagatca cgctgggcgt gtagcgatac agccgctgca
     2461 gatgattcga tgtcagcgag gtgccgagcg tcccggtggc gttgttgatg ccatgctgcg
     2521 cgagcatcac cacgtccatg taaccttcga ccaccagcac gcggtcgagg tcgcgtaccg
     2581 tgcgcctggc ttcgaacagg ccgtagagct cctggctctt gtggaagatc gccgtttccg
     2641 gcgagttcag gtatttcggc ttttcgtcgg tgagcacgcg accgccgaat gcaacggcgc
     2701 ggccccggtt gtcgcggatc gggaacatga tccggtggcg aaagcggtca tacaatccgc
     2761 cgtcgtcgcg ggcaatcagc agcccggcgt cgagcagcag cgtgcggtcc gcctccgagc
     2821 ggccgagcgc gttgagcagg ccgtcccatc ccggaggggc gaagccgatc ccgaagcgct
     2881 cgatcgtttc ctcgctgagg ccgcggcctt gcaggtactg ccgggcttgc gccgcttgcg
     2941 catgggtgcg caattgcgcc tggaaccact tcgcggccgc ttccaggcgc gacgccagct
     3001 ctgcctggcg cggtgaggcg gcggccgccc cgccttcacg ggggacttcc acgccggcta
     3061 cgccggccag cttttcgacg gcggcgggga aatcgagccg ttcgtattcc atcaggaagc
     3121 cgatggcgtt gccaccggcg ccgcagccga agcagtagta gaactgcttg tccgggttca
     3181 cgctgaacga ggggctcttc tcgtcgtgga aagggcagag ggcagagtaa ttcttgccgg
     3241 atttgcgcag gttgacgcgc gaatcgatga catcgacgat gtccacccgc tcaagcaggt
     3301 cgtctatgaa ttgttgcggg attcttcctg acatcgcgga ctgcgcatgg gagtgcgtcg
     3361 gcatgttaca ggaacgcagg agtcgaactc caatcgtcgg gcggggggag cgatcaggtg
     3421 aggcgggcct tgatgcgctg gctgacgagg cccatgtccg ctcttccctg cacctgcggc
     3481 ttgaggatcg ccatgacctt gcccatgtcc ttcatggcca cggcgccgct tgcgtgcatc
     3541 gcgccgtcga tcagcagttc cagttcgggc tcgccgagtg cccggggcag gaactcctgg
     3601 atcaccgcca gttctccggc ctcgatctcc accaggtcca ggcgcccggc gctttcgaac
     3661 tgctggatgg agtcgcgccg ttgcttgatc atcttgtcga ggatcgccag gacgcgcgca
     3721 tcgtccactg cgatgcgctc gtcgacctcg acgcgctgga tttcggcctg gatcaggcgg
     3781 atagcgccca acctggcctt ttcctgcgcc cgcatcgcgg ccttcatggc ctcggttatg
     3841 cgggtcttga gcgtggcgtc gctcatcgga ccgtcgccgg gtgcgggatc agtacaggcg
     3901 ctgctggcga cgcgattcgc ggctcacctt cttcaggtga cgctttacgg cggctgccgc
     3961 cttgcgcttg cgaatccagg tcggcttctc gtagaactcg cgacgacgga cttcagccag
     4021 cacgccggct ttctcgcagg agcgcttgaa gcggcgcagg gccacgtcga agggctcgtt
     4081 gtcttttacc ttgatggacg gcattcagga cctaccagac agttgttcgg atcacgtaac
     4141 gatgctcccg ctacctggag ggcaggcggg gcgccgaatt ctaaaggcaa tgcggccaga
     4201 atgcaaaacg agcgcagcgg ggaatattgc cgccccgtag tggttcgccc cgggcggcgg
     4261 ttatcatccc cgcccatgcg tgtgctaggc atcgaaacgt cctgcgacga gacgggggtt
     4321 gcggtctttg attcggaccg gggcctgctt gcgcattgcc tgcacagcca ggtggccatg
     4381 catgccgaat acggcggcgt ggttcccgag ctcgcgtcac gggatcacat aaagaaagcg
     4441 ctgccgttga tccggcgggt actgcaggag gccgcgtgtc ccgtggagac catcgacggg
     4501 gtggcgtaca cggcagggcc tggcctgctg ggcgccctgc tcgtgggtgg ctgcgtcgca
     4561 cggtcactcg cctttgcatg gggtgtcccg gcggtcgggg tgcatcacat ggaggcgcat
     4621 ctgctggcgc ccatgctgga accggagcgt ccggtctttc ccttcgtggc cttgctcgtt
     4681 tcgggcggac acacccagct ggtgctggtc gagggaatcg gggcctatcg aatcctcggg
     4741 gagtctctcg acgatgcggc gggcgaagcc ttcgacaagg tggccaagat gctggggctc
     4801 ggttatccgg gcgggccgca gctggccgcg ctcgctgccg acggcgtgcc ggggcggatc
     4861 gattttcccc gcccgatgac caaccggccc ggactcgatt tcagtttcag cggactgaag
     4921 accgctgccg ccaacgcact tcgagggctg cagcaggccg gcggcgtcat cgcgccacag
     4981 tgcaaggccg atatcgcgca cggattccag caggccgtga tcgacaccct cctcatcaag
     5041 tgccggcgtg cgctgcaatc gaccgggttg acgcaactgg tgctggcggg cggcgtcagc
     5101 gcgaaccgcg agctgcgtga acgcttgcgc gatgaggcgc aggcgcgcgg ctggcgggtc
     5161 ttctatccgc gtgcggagtt ctgcaccgac aatggcgcca tggtggcgtt cgccggttgc
     5221 cagcgcctgc tcgccggcca gcacgacgat ctcgcggtgc gcgcccaggc gcgctggccc
     5281 atcgatacgc tggaaccggt ggcatgatcg cgtcgtgcac cggccgcggc agatcgtgat
     5341 ggacgtcgtc tatatccgcg gcttgcgggt ggatacggtg atcggcgtct acccgtggga
     5401 acgcacggtc cggcaggtgc tggagttcga tctcgagatg gcgagagata ccgccgccgc
     5461 ggcacgctcc gatgcgatcg ccgacgcgct cgattaccac gccgtcgccg agcgtgtccg
     5521 cgcgctggcc gcggtgagtt cctgccagct ggtggaaacg ctcgccgaga ccgtcgcgac
     5581 cgcgttgatg gcggaattcg ccgtgccgtg gctgcggatc aagctcgaca agcctggcgc
     5641 cgtgcccggt gcccggggcg tcggggtgtc gatcgagcgc ggcgtccggc gcgatcaata
     5701 gaggttgcgc ccgccatcca ccgcgaggat ctgcccggtc acgtagggcg cctccaggcc
     5761 caggaaccgc gccgctccgg caatgtcttc gggcgtgccc attcgcttca gcgcggtctg
     5821 tgccagcagg gcatcggcat gcgcagctcc aagcaccgcg ttcggctgct ccggccacag
     5881 gatcgcgccc ggagcgatgc cgttgaccct gaccgacggg ccgagttcaa ccgccagcgc
     5941 gcgcgtcatc attgcgagcc ccgccttggc catcgcgtac acggtgtgct ggggcagcgg
     6001 aatgtccgca tggatgtcga cgatgttgac tatcgatccc tgcctgcgcg cgagcgccgg
     6061 cgccagcgcc tggctgagga aaaacgcgcc cttcaggttg gcgtcgaaca attcgttcca
     6121 ttgcgcatcg gtcacgttgc caagcggggt cgcgtagaaa gccgaggcat tgttcaccag
     6181 caggtcgagt ccgggggcga gtgcccaggc ttgcgcggcc agctcccgga catcgttcgc
     6241 ccggcacagg tccgcctgca cggcggcaca gctgtcgggg cgcacggcat tgagctcgcg
     6301 cagcagctgc tgcgcgcccg cggccgagct gcggtaatga atcagtacgc ggcacccggc
     6361 gcgatgcagc tcgcgcgcga tcgccgcgcc gatgcgatgc gctgcgccgg tgaccagtgc
     6421 gacgcgcccg gagctgctat tccccatgat cctctccctc gagccgcgcg acctcgatgc
     6481 agcgcaatcg atgcatctcg acagcgattt gcggcccgtg caggccgcgt gccagtgcgg
     6541 atgcggccgt taccgtggcc gcggcggcgc gggcgcgcct gagcttttcg gcctgtgcat
     6601 aggggcgctg ctccagtccg gtgcgcccgc gcgcgtccgc ctcgcaggcc gccaggaatt
     6661 cctcgaagcg ctcgccgttg cgcagcgcgt ccacgtcctc gagcagcgac acgatcgcgg
     6721 gcgcgcgcat ttccagcacg cgatggcagc gcgtgtggtg caggcatacc acgagcgcca
     6781 gttcgcggta cttgaccggc acgcgcagtc gttcacacag cgcccgcacc gcgaccgcgc
     6841 cgagcttctc gtggccgacg tgggagggcc agcgttcgcg cggcgtgagc gccttgccga
     6901 gatcgtgcac cagcaccgcg aagcgcgccg gctccgtcag ccgcagttgc gcggcgaggc
     6961 gcaggcagag caatacgtgt tcgccggtgt cgatctcggg gtgatggcgt gccggttgcg
     7021 gcacgccgaa cagcgcgtcg atctcgggaa atatcgcccc gagcgccccg cattcgcgca
     7081 gcgtgcgaaa gtattcctgc ggactcgcct cgccgagcgc tcggcgggtt tcggtccaca
     7141 cccgttccgc gaccaggtgc gccaactcgc cgctggcggc catgtcgcgc atcagcgtca
     7201 tggtggcggg gtgaacgcga aagccacgtg acgcgaacct tgccatgaag cgcgccacgc
     7261 gcagcacccg caacggatcc tccacgaagg catccgatac atggcgcagt acgccatcgc
     7321 gcacatcctg agcgccgccg taagggtcga tcaggtggcc gtcccggtcc tcggccatcg
     7381 cattgatgct gaggtcgcgc cgcgccaggt cgtcctcgag cgagacctgc gagccggtct
     7441 cgcaggtgaa gcccgcgtag ccgtgtccgc tcttgcgttc gcgccgcgcc agcgcgtact
     7501 cctcgctggt ttcgggatgc aggaacacgg ggaaatcgcg cccgatctgg cgaaagccga
     7561 gtgcgagcat ctgctcgggg ctggcgccca cgacgaccca gtcccgttcg catggcggga
     7621 tgtcgagcat gcggtcgcgc accgcgccgc cgaccagata gatttgcacc gtgtttgccg
     7681 tggcctgaca gaaaggcagt ttagcaattc gcaggagctg caggtccggc ttcggccgga
     7741 caattgtccg catgaatgcg gacctaccgc ggcacatgtc cgcatgaatg cggacccaca
     7801 gggattgttc agcaattgca gtgaaacagc gcgccgtcct cgaggcgcat ggcgcgcaac
     7861 cggcctcccc atacgcagcc ggtgtcgagg gcgtgcacgt cggcgcgctc cgcccggccc
     7921 tccagcgcgg cccagtggcc gaacacgacc gggacgcccg cgctgcgcct cagcgcgtgg
     7981 gcgtaccacg gcgcgaagcc gggattggcg tccgcgggcg ggctcttgtt gtcgagatcc
     8041 agcttgccgt cggcggcgca gaagcgcatg cgggtgaggt agttggtgat cagccgcagt
     8101 cgctcgaatc cttcgagccg ctcgtcccag gtgtcgggga aattgccgta catgtggtgt
     8161 aggaagtccc ggtacagcgg cccctgcagc gtggcctcca cttcgcgcga acgcgcgagg
     8221 gtgtcctcga cgctccactg cggtggtatg cccgcgtgca ccatcacgtg cgtgaaatcg
     8281 ctcccgcggt aggccagggg ctggcagcgc aaccagccga ggagttcctc gcgatccggc
     8341 gcctcgagaa tcgcgcccaa cgaatccttg cgcagtgcct tgcgcacgcc ctcggatacc
     8401 gcgagcaggt gcaggtcgtg gttgccgagc accatcaccg cgcgctcgcc gagcccgcgc
     8461 acgaagcgca gcacctgcag cgattcgggg ccgcggttga ccaggtcgcc ggcgaaccac
     8521 agccgatcgg ccgtcggatc gaaacgcagg gtgtcgagca atcgcagcag cggctgatgg
     8581 cagccctgca cgtcgccgat cgcgtaggtt gccatcaggc gccagcgcct ggttgcggcg
     8641 cgacggcgtt gctgagcgcg acgaactccc ccacggtgag cgtctccggg cgccggcccg
     8701 gatcgatgcc caggcgctcc agcgtcgcgg catcgagcag gcccgcaacc gcgttgcgca
     8761 gcgtcttgcg ccgctgcccg aaggcctggc ggaccacctg ctccagcatg cggtagtcgt
     8821 gcgcgcgtcc gtgcggatgc gactggggca gcagtcgcac gatggccgac accacgcgcg
     8881 gcgggggatg gaaggccccg ggcggcacct cgaacagggg ctgcaccgcg cagcggtact
     8941 gcaccatcac gctgaggcga ccgtaatcgg cgctgcccgg ttcggcaccg agtcgctcga
     9001 ccacctcgcg ctgcagcatg aaatgcatgt cgcgcacgca gtcgccctgc tccagcaggt
     9061 ggaacagcag cggcgtcgag atgttgtagg gcaggttgcc cacgacgcgc agcccgcctc
     9121 cgggcggggc cagcgcgcgg aaatcgaagt cgagcgcatc ggcggcgtgc acgctgaagc
     9181 gctcactctc gtgcaggcgc catcgggcca ccaggtcgcg atccagttcc accacgtgca
     9241 gcctggcgcc gctgcgcagc agcggttcgg tcagtgcccc gtggcccggg ccgatctccg
     9301 cgatggtgtc ttcggcgcgg gcgtcgatcg cctgcatgat gcggtcgatc acgtgacgat
     9361 cggtcaggaa gttctgcccg aaacgcttgc gcggacggtg ccccgccggg ccaccggcca
     9421 tcagccgcgc tccgccgcgc gcagggcggc gctgcgcgcg gccatgtcgt gcgccgtctg
     9481 cagggcgaac tgcaggctgc cggtgtccgc cttgcccgtg cccgcgaggt ccagcgccgt
     9541 gccgtggtcc acggaggtgc ggatgatcgg cagaccgagc gtgatattga ccgcctggcc
     9601 gaagcccttg tacttcagca ccggcaggcc ctggtcgtgg tacatcgcga gcacggcgtc
     9661 ggcgccctcg agatgatgcg gtgtgaacag ggtatcggcg ggcagcggac cgatcagcgt
     9721 catgccctcg gcacgcaggc gctgcaggca ggggatgatc acgtcgatct cctcgcgacc
     9781 caggtggccg ccttcgcccg cgtgcgggtt cagccccgcg accaggaccc gcgggtcgcg
     9841 gataccgaaa tgccggcgca ggtcgcggtg caggatgcgc agcacctgtt ccagcgattg
     9901 tgccgtgatg tggtcgctga cgtcgcgcaa gggcaggtgc gtggtcgcca gcgcaacccg
     9961 caatccgggc gtggcgagca tcatcaccac gcgtggcgtg ccggtggcgg cggcgaggaa
    10021 ttcggtgtgg ccgctgaagg gcacgccggc atcgttgatg attcccttgt gcaccgggcc
    10081 cgtcaccagc gcggccacgc gccgtttcat gcaggcgctt acggcggcct gcagcgtcgc
    10141 gagcacatag ggcgcgttgg ccttgtcgag cgtcccgggg acgacggggc gtccgagcgc
    10201 aaccggcagc acgcgcagcg tgcgtggcgg cgcgggcagg gggtccggcg gcaggatgtc
    10261 cagcggcaac cccagtgcgc gcgcacgatc cagcagcagt tgcgggtcgg cgatggcgat
    10321 cacgctggcg tcgaaaccgc gctgcgccag cgtaaccacc aggtcgggac cgatgccggc
    10381 cggttctccc ggggtcagcg cgagcaccgg cgcatcggcg cgggcatcag atcttgattt
    10441 cgacaaaagc ctcgtcccgg atcttctgca gccacgtgtt cagttcgtcg tcgtacttct
    10501 ggccgaacag gatgttgcgt gcctggttgc gtttcatctc gtcgctgaga tcctgggtgc
    10561 ggcgctcctc gacctgcagc acgtgccagc cgaaactgct gcgcaccggt gcgctgatct
    10621 cgccggcccc ggtctggttc atggcctgct cgaactccgg aaccatctgt cccgcgctgg
    10681 tccagccgag gtccccgcct tcgcgcgccg agccgatgtc ttccgagaat tcgcgcgcca
    10741 gtttcgcgaa gtcctcgccc gccagcacgc gcgcgcgcaa ctgcgccgcc agctgctgcg
    10801 cctgcgcgtc gctgcgcacc tcggagggct tgatcaggat atggcgcacg cgggtctgct
    10861 gggtcatctg tccgctgccg ccgcgtttct cgaacagctt caccaggtgc aggccgctgg
    10921 cgctgcgcac cggtgcggat atcgcgccgg tctcgagcgc cggaaccgtc tccaggaaca
    10981 cgctcggcag gtcgccggcg aggcgccagc cgagatcgcc gccggtgaac gcgtaggggg
    11041 tcttgtcggg cgcgcgcatg aagcgctcga aggcggtgcc ggaggccagc cgcgaacgca
    11101 gcccctccat gtaggccatg gcggcgcgtt cctgctccat cgtggcgtcc gtggccagcg
    11161 gcaggagcag gtgcccgatg tggtagacgg ctgccgtgcg cttctggcct tcttccgagg
    11221 caaggtaatc atcgacctcc tgctcggtca cctggacgcg attgcgcacg ttgccctgct
    11281 gcacgcgcga gaggatcatt tcctggcgta cctgctcgcg taccgccgcg tacgagccgc
    11341 cgtcggcctc gatctgggcg cggaactgct ccaggctcgc gccgctctgg cgcgccatgt
    11401 tggtgagcgc ctcgttcagc tgctcgtcgc tgatgcgcac cccggcgcga tcgcccatgc
    11461 gcagctggat gcgctcgagg atgaggcggt cgagcacctg gcgcgccagc acctcctgcg
    11521 gcggcatttc gatcttctgc cgcgcgaggt tctcggtgac ctggcgcatg cggtcctggt
    11581 attcgctcgc gagaatgatg tcgtcgtcga cgatcgcgac cacgccctcg agctgccgcg
    11641 tctgcgccag cgcgcccccg gccagtcccc agcagcacag cactatcgcc gcggcagcgc
    11701 gccaccggct tcccgctcca gtcgattcaa tacgcaaggc cgttgtcctc caggttgctg
    11761 ccaaagccga agatgctgtc ctgcaacagc tggtcgatgt tcttgccgag gccgccgagg
    11821 ccgcgcagca cgaactgcag gtagaagccg tagtcgcgac cctgatccca gtccacgcct
    11881 tcctggtaca ccatgcgcac cgaccagcag caggagctgt attccagccc cgccagctcc
    11941 tcgaggctgt agtcgttggt gaaatcgtac tggtagcggg tgaatacccg ccagcggtcg
    12001 ctgatgggca atacggccga aaagtcggcc tggtcgattg tctcgctgat caggttgccg
    12061 aaaatgtcat aggaggtctg ctcgcggcga tagcgatagc ccgcgttcac gatcgtctgt
    12121 gtatcggggg tccagtgaac gaaggcgccg ccttcgttca cctgctcgcg atgcacgtcc
    12181 agcagcgtcg tgcccgtgac cgagacgctg cgcgtcggcc ggacctgcac ctccgcggca
    12241 atttccgagc tggagaccga ctgcgcatcc gcgggctgcg cgtcgctcca tccgcccgag
    12301 ggcggcacgc gcgtgcggca gctcggctgg gactgcgcgc gctgcgcatc gcagaccgtc
    12361 acgaggcgat cctcgaaata gtagatctgg ccgacgctgg ccgtcacccg ttccatgccg
    12421 ttctcgtcgt cgatgaggcg cgaggtgacg ctgagcgagg cctggttggc gtcggcaatg
    12481 cggtcgcgcc cgctgaagcg cgtggtgcgg aacagctggc tgaagctgaa ggtgaggtcg
    12541 gaagagtcga agttcggcag gtcgctgtgg tcctcccggt ccacccacag gtagaacgcc
    12601 cgcggctcca gcgtctgcag gaacgcggtg ccgaaaaggc tcgtgtcgcg ttcgaggaaa
    12661 tagccggcat ccaggttgac catcgccgcg ccgaccgacg gggattcgtc acccggcagc
    12721 gtgaggctct cgtccaggtt gtagctgatc gactggtagc ccagtgccgg ccgcacgaag
    12781 gccgcggccc actgcatcgg gaaggcgatc tgtggttcca ggtagagccg ctgtccggtg
    12841 agctccgcgc gatccttgtg ctcgaagacc gtgaactcgc tggtgagcga gtaatcgagc
    12901 gcgaacggag tgccgctgaa cggacggttc atttcgaggc gcggcatgat cttgtagggc
    12961 tcggcgagcg ctcgatccag cagccggtac tgttgcgcct tcgccgccag gacccagctg
    13021 tcgtcgagtc ggtacgtgac ctggcccgtt tgcggcagga agatggatcg ttgcacttcg
    13081 agcccggtcg agctcagctg gcgcaggtag tcctcgtcgc tcacctcggt gtagctgatc
    13141 agcgtgccca tgcgctcgac cggggcgccc gtgtgctcca ggctggcgat ccagcggtcc
    13201 tcgtcggcga cctcgtcatt cggcaggatg gcgccgctgg tggcccagga actccagcga
    13261 ttcaggtagc gcaactcccc cccgatcatg gtgccgcgat cgttcatgta gcgcggcagc
    13321 agggtggcgt cgtagttcgg tgcgaggttg aaatagtagg gagtggtgac gtccaggccg
    13381 ttctgggacg agttcgtgaa cgtgggccag agcagcccgc tcatgcgccg gtcatcgacg
    13441 ggaaagctca ggtacggcgt gtagagcacc ggtatgccct tgacctcgag ccgggcattt
    13501 tgcgcggtgc cccgcccggt ttcgcgatcc acgcgcatcg agtcggccgc gagctgccag
    13561 tcgtcgtggc cgggttcgca ctgcgtgtag acggtctcct cgaattcgag ggtgcggtcg
    13621 acgttgcgcc gcaccagttt cgcttcgccg cgcgcgtgct ctcggtgcat cacgaagcgc
    13681 gcttcacgta attcgccgga gttctgcgcc gtgtcgatcg atgcggactc gcctcgtacc
    13741 agcaggcccg gttcgcggta ctgcacgttg ccctcgatat cggcgcggtc ctgttcgcgg
    13801 taataagtcg cgcgatcggc acgcaggcgc cgatcgccct gcagcagtgt tacgttgccg
    13861 gaaaatttcg cgatttccgg gttctgcagc agttcggatt cggccgacga ggcgtggacg
    13921 gtgccaatct gcgctccgcc ttcggcggga ggctccagcg gcggctcgat ataagcgccc
    13981 cgacattgtg ccccgatcgc cgcggcctgc tccgggctca ggcgccgcag tgtcacccag
    14041 tccatggtct cggcgagcag atcggcatcc gccaacccgg ccaccgatcc tgccggcgcg
    14101 gcatcgggcg cagcagccgg cacagcatcg agcgctgcat ccggtgtgac atcgggcgtt
    14161 gcatccgctg ctacattcgg cgcggcgctc ggggtggatc gcaccgcgcc gctcgcaatc
    14221 gtcggcgcaa cggcgctgca gtcccagccg cccgtctccg cgccccggca ttgccattgc
    14281 ccgttgtcgg ccgcagcggc gtgcagcacc gcggcgctcg ccagcccggc gatgacgcgg
    14341 gaaaggaaaa tgacgggctt gctctgcatt ccgttgaagt ccgggcctcg ggttggatca
    14401 gccgcggatc ttttcagctc cggggaagcg tggataatgc acgacgctct ggccgagggc
    14461 cgcattatag cggcatcgcg tcacgaggat cgactggcgg ggcgctgatt gtgcggcgtc
    14521 ctcgccttgc cgcgttaccc tgaccggaat cgacaatgga cgcgttgctg cagtggtgtt
    14581 cgaagctggt cgagccggtc gcggggcgac gcctgggcct gtccgcggtg gcgggcgatg
    14641 cgagtttccg gcgctatttc cgcctcgagg gctgcgagcc cacgcgcatc gccgtattcg
    14701 caccgccgga caaggagcgc aacctcgagt tcacgcggat tgccgcgatg ctgcgcgagg
    14761 ccggggtgtg cgcccccgag gtcatcgccc atgacagcgc gagcggtttc ctgctgctga
    14821 gcgatttcgg cgataccctg atgctgggcg cgctcgatga gcacagcgtc gatcgcctgt
    14881 atgcgcgggc gatggatacg ctcctgcgca tccagggcac cgcgaccgcg tgcgagggtt
    14941 ggcatctgcc ggcgtattcc ggggcgctgc tgctgcagga aatgcagttg tttcccgact
    15001 ggtacctgaa agggctgctc gggatcgcgc tgggcgcgca cgacagggcc ctgctgcagg
    15061 aactgttcgc gcgcctggtt gccagcgcgc aggagcagcc gcaggtcttc gtgcatcgcg
    15121 actaccattc gcgcaacctc atgctgctgc ccggcggtgc gctgggcgtg atcgatttcc
    15181 aggacgccgt gaccggcccg ctcacctatg acctggtttc gctgctgcgc gactgttacg
    15241 tgcagtgggc gccggcgcgg gtcgaggcct gggcggcctc cttcgcggcg cgcgcggtcg
    15301 cggcaggctc gatgcagccc gtgcccgcgg ccacgttccg gcgctggttc gactggatgg
    15361 ggctgcagcg gcacatcaag gtgctcggca tcttcgcgcg gctctggctg cgcgacggca
    15421 agcccggcta cctgcacgac ctgccgctgg tgatgcgtta cacgctcgac gtggcggcgg
    15481 cccatccgga aacggcgccg ttcgcggact ggttgcgcga tcgcgtgctg ccggtcgcgc
    15541 gcgggcagga ctggtaccgc gcgcgatgaa ggcgatgatc ctcgccgcgg gactcggcac
    15601 gcgtctgcgc ccgctgaccg aaacgctgcc caagccgctg ttgccgctcg ccggcaagcc
    15661 catgatcgtc taccacgtcg agaagctcgc cgccgccggc ctgaccgagc tcgtggtcaa
    15721 tgtgtcatgg ctcggtgcgc tgatcgagca gcatctgggc gacggcgagg cgttcggcgt
    15781 ggccatccgc tacagccgcg agcccgaagg cccgctcgac accgggggcg gaatccgccg
    15841 tgccctgccg ttgctagggg agcagccgtt cctgctggtg gccggtgaca tctggagcga
    15901 ttacccgctg gcgcggctcg ctgcccgcgc gctggccgag ggcgaactgg ggcggctggt
    15961 gctggtgccg aacccgccgc agcacgctgg cggcgatttc agcctcgacg cggcgggcag
    16021 ggtgggcact tcggcggtgg ggcgacgctg cacgtattcg ggcatctcgc tgctgtcgcc
    16081 gcggctgctg gacggctgga gcgacacggt gttcccgctg cgcgagccgc tgcgccaggc
    16141 ggccggccgc ggtgtgctga gcggcgaaat ctggaccggc gactgggagg acgtcggcac
    16201 gccggagcgc tacgccgcgc tcaaccggcg cctggaccgg cgcccggagt cgcctccagc
    16261 gtagtcagcc agccgcgcag gcggcgcgtc agcggcgcat cggcgggccc ggggtgccag
    16321 ctcccggtct ccagcgtgag ctggcggtaa tgcttcaact gcagcgcggc ggcctgttca
    16381 cggcgcagcc ggccctgctc acgaggcgca gtcgggctca gtacctccag caccggcctg
    16441 gccaggcccc tgggccactc gagcacgagc cccggcaatt ccaccggatc cagcagcgcc
    16501 gcggcccgca ccgagccggg cgcgctgccc atgccacggg tcagcagcac ggcggcgctg
    16561 ccctcggcga ccaggaccag cgggcggccc tcggccgcgg cggaggctat cgcggcgagg
    16621 atgcgcgcct ggctgcgcac cgcccattgc tccagtgcgc cggcacgccg ggcgttgagc
    16681 gttgcggacc ccgcgccggc gggcgcggat tccgtagcag cgggcggcgc agccccgggt
    16741 gtgccgattt gctccgcatg gatgcgcggt ggcggctcca gtgtcagcag ccacgtggcc
    16801 cagccatgcg ccggcatgga ctgacggatc tgttccaggc ccgccgccga ggtgggatga
    16861 ctgccgtcgc ccgggatcag caccagcgcg ccccgcgcca ccggcccgcg ggcttgcgtg
    16921 acgaacaccg cgaactcgtg gccgtccgct ttcaggatgc gccgttcctg ggccagcacg
    16981 acggccagcg cgtccagatc ccgatcgatc gcgggcgcgg gttcgggttc gggtgcggcg
    17041 ggtgcggcgg gtacggcggg tgcggcagcc ggcacgggtg atgctgctgg ggccggcgct
    17101 tgcggctcgg ccgcgttgcc gggcgggccc gcgatcacga ggcccaggag cagtggctga
    17161 aggaggcgtg tggtgcggcg catggccgca gtatagccac cgcggcggcc gtcccggcgc
    17221 gggaattcgg cggtttcatc cagtacaatc gcgggccttc ccctgcgccc gtggacgact
    17281 cgcgatgaac gacttccaga ttgccccctc gatcctgtcg gccaacttcg cgcgcctggg
    17341 cgaggaagtg gacgccgtgc tcgcggcggg cgccgacatc gtgcatttcg acgtcatgga
    17401 caaccactac gtgccgaacc tgacgatcgg cccgatggtg tgccgcgcgc tgcgcgatca
    17461 cggggtcagc gcgcccatcg acgtgcacct gatggtcacg ccggtcgatg cgatcgccgc
    17521 cgcctttgcc gaggcgggcg ccacgtacat caccttccac cccgacgcga gcacgcacgt
    17581 ggatcgcacc ctgcagctga tccgcgacgc cggctgcaag gcggggctgg tgttcaatcc
    17641 ggccgccggc ctggatgccg cgcgctacgt gatggacaag ctcgacatga tcctgctgat
    17701 gtcggtgaac cccggcttcg gcggccagaa gttcattccc gccacgctgg ggaagctgcg
    17761 cgaggcgcgc gcgctgatcg acgccagcgg ctttccgatc cgcctcgaga tcgatggcgg
    17821 tgtgggcact ggcaacatcc gcgcgattgc cgaggcgggc gcggacacct tcgtggccgg
    17881 ctcggcgatc ttcggcgcgc cggactatgc cgcggtgatc gccgggatgc gcgcggagct
    17941 cgcggccgcg cggcggccct gagacggtgg cgcacgccct gatccggcgc ctgctcgacg
    18001 gccgcctgcc cgcgctcgcg atgttcgatc tcgatggcac gctggtcgac agcgtgccgg
    18061 acatcgcccg cgcgctcgac gcgacgcttg ccgcgcgggg cttcgcgccg gccggcagcg
    18121 agcgcgtgcg ccactgggtg ggtcgcggct cgcgcaacct ggtgcgcgag gcgctggcgc
    18181 atgccgtgga tgccgcgcct gaacacgtcg ccgaggacct gctcgacgag gcgctcgggt
    18241 tctaccttgc gcgttacctc gaggactgcg ccggcgcgac cacgctgatg ccgggcgccg
    18301 cggcgctgct ggcctcgtta cgcgcggccg gtgtggcgct ggcctgcgtc accaacaagc
    18361 cgatcgcgat caccgaacgc ctgctcggcg cgctgctgcc ggatgcgggt ttcgcggcgg
    18421 tgctcggtgg cgattcgggc gccggcgtga agcccgcgcc ggggccgttg ctcgcggcga
    18481 tggcgaaggc gcggacgctg ccgtgcgcgg cgctgatgat cggcgattca cgccacgacg
    18541 tgcatgccgc gcgcgcggca ggggtgcgcg tgatctgtgt cgcgggcggc tacaaccacg
    18601 gcgaggacat ccgccgcgag gcgccggacc tggtcgtcga cgggctcggc gaattgcttt
    18661 gaatgcccct tgcagtagaa tctgtcgcag cacatttccc cacgggagca gacatgaggg
    18721 cgatccggcg ctggcgatgg tgacagtcgc gattacgcgc gctcgtgcgc gtgccttttg
    18781 ccatttcccc gagtttccgg agtccccatg aacgccaccc gcctcgaaca gctcgcccgc
    18841 gagggcttca cccgcgtccc cgtcgtccgg cgcctgctcg ccgacctcga gacgccgctc
    18901 ggcgcctacc tgaaattcgc cgggccgttc agcttcctgt tcgagtcggt cgagggcggc
    18961 gagaagtggg ggcgctactc gatcatcggc ctgccgtgcc ggcggcgcct cgtggtccgc
    19021 ggtactcgga tcacgctctg cgacggggac gcggagcagg acgtcgcgca cgccgatccg
    19081 ctcgccttcg tcgaggaatt cctcgcgcgc gagcgcgcgc tcccgcacga ggacctgccg
    19141 atgttcgcgg gcggcctcgt gggctatttc ggctacgact gcgtgcgcat ggtcgagccg
    19201 cgcctcgccg cgagcgcgcc gcccgacgtg ctgggcacgc ccgacatcct gctgatggtc
    19261 gccgaggaat tcctggtctt cgacaacctg gccggtgcca tcgcgctggt cgtaacggtg
    19321 gacccgcgcg aggcggacgc cgcggccggt gccgaggcgc ggctcgatgc gctgcagcag
    19381 cgcctggagg ggccggcgcc gcggctgccg ccggtggaca tcgatgccgg gcggcgcgac
    19441 agccagcgtg tgcgcgacgt cgaggcggcg ttcgagtcgg agttcggccg cgagcgtttc
    19501 gaggccgcgg tggagcgcat ccgcgaatac atcctcgccg gcgacgtgat gcaggtggtg
    19561 ccctcgcagc gcatgagcgc gccgttcagc gccagtccgc tgaatcttta ccgtgctctg
    19621 cgcaacctca atccctcgcc gtacctgtac ttcctcgatc tcggcgattt ccagatcgtg
    19681 ggctcctcgc cggagatcct gtcgcgcctg caggacggcg aggtcaccgt gcggccgatc
    19741 gcgggcacgc gccggcgcgg cgccacgcac gaggaggacc tcgcgctcga ggccgaactg
    19801 ctggccgatc cgaaggagat cgccgagcac ctgatgctga tcgacctcgg gcgcaacgac
    19861 gtggggcgcg tggccgggat cggcagcgtg acggtatcgg agcgcatgat cgtcgagcgc
    19921 tactcgcacg tgatgcacat cgtctccaac gtctcgggcc gcctgcgcga gggcctgagc
    19981 gccatcgacg tgctgcgcgc cacgctgccg gcaggcacgc tgtcgggcgc gccgaaaatc
    20041 cgcgcgatgg agatcatcga cgaactcgag cccacgcggc gcggcatcta cggcggcgcg
    20101 gtgggttacc tctcgtggca cggcgagatg gacacggcca tcgcgatccg caccgcggtg
    20161 atcaaggacg gacgcctgca cgtgcaggcc ggcgccggga tcgtcgccga ctcggtgccc
    20221 gcgctggagt gggaagagac catgaacaag gcccgcgcga tgttccgcgc cgccgcgatg
    20281 ctctcctgag gctgcggcgg cggtgctcgc cctgtccgcg atggactggc tcgcgttcgc
    20341 gggtaccttc gccttcgccg tgagcggcgc gctgctcgcc gcgcgacacc gcctcgacat
    20401 catcggcttc ctgttcgtgg ccaacatgac cggtgtcggc ggcggcaccg tgcgcgacct
    20461 gctgctggac gcgccggtgt tctgggtgca ggacgcgacg tacatcgtgg tctgcagtgc
    20521 cgccgcgctc ctcacttatg cggccgcatc gctgctcgat cgcgcctccc aggccttgct
    20581 gtgggccgat gcgataggtg tggcgctgtt cgcggtgctc ggcgcgcaga aggcgctgct
    20641 ggccggcgtg gggcccggtg tcgccgtggt catggggatt ttcaccgcct gcttcggcgg
    20701 catcatccgc gacttcacgc tgaacgagcc gcccatcatc atgagtcgcg agatctatat
    20761 aaccgccacg ctggtgggtg ccgtgtgtta cgtgctgctg gccgggctgc gcggcgcgca
    20821 ggacatcgtg tcggtcgtcg gcggcaccgc ggtcggtttc acggtgcgcg cactggccat
    20881 cgtggccagg ctctcgctgc cctcgcatgc cggcctcggc cacaaggacg ggggcagcgc
    20941 acaatgagcg cggcctgccg cctgccgcct gccgcgcacg cgcggcgcgc cgctcagggg
    21001 gcgaccggca ggctgatgcg gcgctccggc ccgccgaaga tgccgaccca gcccgcgccg
    21061 tcctcgccca ccgtgccccg gtacataccg ctggtgttga agcgcatcgc gtaatcgccc
    21121 ttcgcgtcga gcacgatcag gccgccgtcg ccgccgatcg cggccacctc ggccagggtg
    21181 gcgtcggccg catctgcggc ggtcttgccg acgaggcgga ccagttcggc gacgctgtgc
    21241 gcggcggccg tgcggatgaa catctccccg gtgccggtcg ccgacaccgc gaccgagcgg
    21301 ttgtcggccc aggtgccggc gccgatcacc ggcgagtcgc cgatgcgtcc gtagcgcttg
    21361 ttcgtcagtc cgccggtcga ggtgccggcg gcgagattgc cgtgccggtc cagcgccacg
    21421 gcgccgacgg tgccgcgctt gtcgtcctcg cggtaggcgc tcgccggttg cgcgtcgtga
    21481 tcgcgctcga tgcgctcccc ggcgattgcc cgctgcaa
//
LOCUS       DAORUQ010000033        20666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2188_length_20666_cov_7.078890, whole genome shotgun sequence.
ACCESSION   DAORUQ010000033 DAORUQ010000000
VERSION     DAORUQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 20666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20666
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2188_length_20666_cov_7.078890"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLW13_04865"
     CDS             complement(<1..384)
                     /locus_tag="PLW13_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQY69733.1"
                     /translation="MSDRPNTRNMALFCDFENVALGVRDAKYAAFDIRKVLERLLLKG
                     NIVVKKAYCDWERYKEFKKAMHEASFELIEIPHVRQSGKNSADIRMVVDALDLCYTKS
                     HVDMFVIISGDSDFSPLVSKLRENNK"
     gene            complement(394..954)
                     /locus_tag="PLW13_04870"
     CDS             complement(394..954)
                     /locus_tag="PLW13_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQY69734.1"
                     /translation="MDVTDGCARYALRYWLRDPQVDDATDSRVRAHALAALDRAGIHL
                     ASRREERLVFAEDEARRAALAAAEIARRMNTLRMVPLFGSLSDDERAQVAQHLVHAPF
                     LAGDVVTRQGAVAHWLYLVIEGAAEICDETGGTRRYVSSLGPGDIFRERGMLTGELRG
                     SAAGAHAAASARTDMLARIRAFFGLD"
     gene            1367..1795
                     /locus_tag="PLW13_04875"
     CDS             1367..1795
                     /locus_tag="PLW13_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQY69735.1"
                     /translation="MLKQVELKHYMVQNPVKVRADANLLDAIHLILVNKISGLCVVDE
                     RNKLIGVLSETDCLRGVLSATYNDSGIGSVGEFMSSQNLHTASPGDHIVDIAADMLKH
                     KIRRRPVIDSDGTLLGQITIRQILRAVKEFSSPKDRTERD"
     gene            1983..2471
                     /locus_tag="PLW13_04880"
     CDS             1983..2471
                     /locus_tag="PLW13_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY69736.1"
                     /translation="MQAQELPEAVPMPSAALPSVELPTVALPPGLDRVLRDYELAWMQ
                     KQPSALAGMFTMDGMALHSGSPPARGAARISAGYAEAAGTPLSLRALAYAVSHDMAYI
                     VGAFAPAAGEPDHGKFVLVLRRGSDGDWKIAADIDNMNALPGAPTPEPEQSNAPVSVP
                     EP"
     gene            2526..2903
                     /locus_tag="PLW13_04885"
     CDS             2526..2903
                     /locus_tag="PLW13_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY69737.1"
                     /translation="MLHHISFGVRDLALSGAFYDAALGALGFRRVFEAGNAIGYGLLD
                     GEDVLYLQQRPDAAAPGPGFHLAFAAPSREAVDAFHACALRAGGTDNGAPGRRPHYGP
                     HYYAAFLVDPDGHRIEAVCKAAG"
     gene            2976..3416
                     /locus_tag="PLW13_04890"
     CDS             2976..3416
                     /locus_tag="PLW13_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQY69738.1"
                     /translation="MTDPFLLAAIEEAECGLGEGGIPIGSVLVHDGAIVGRGHNRRVQ
                     QGSTILHAEMDALENAGRRDAAFYRECTLYTTLSPCAMCSGAVVLYGIPRLVVGENRS
                     YMGEEQWLASRGVRIRVIQDARCIALMREFMQRHPGLWAEDIGE"
     gene            3455..3712
                     /locus_tag="PLW13_04895"
     CDS             3455..3712
                     /locus_tag="PLW13_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69739.1"
                     /translation="MLTLKQLETYEACGGDADGWTRRGGSPGMDYEHWLLIDELLQGL
                     HLIRSGLASQRYADRIRGALLAVTDDEAVRHKLAQMSGDMT"
     gene            3709..4356
                     /locus_tag="PLW13_04900"
     CDS             3709..4356
                     /locus_tag="PLW13_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HQY69740.1"
                     /translation="MKAGPGILFAVSLLLGACSSAYYGTMEKLGVHKRDIMVDRVVAA
                     RDAQTEAKEQFLTAMEQFRGVVAFQGGDLEREYHKLDATLQESEARAAAVRERISAVE
                     DVSGALFSEWEAELGQYSSDTLRQASQRKYDATKRKYTQLLAAMKRAEARLEPVLVPL
                     RDQVLFMKHNLNARAIAGLSEEVVSVQANVDRLVREMESAITQADAFIAELRAEE"
     gene            4382..4810
                     /locus_tag="PLW13_04905"
     CDS             4382..4810
                     /locus_tag="PLW13_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69741.1"
                     /translation="MSVCLAASLALQGGALVYAGPGGAAWRVDADSIRAVGELTTAGC
                     GEDHLLAFVVDAGGGWFQASRGALGADEALAALGLRLGGAIVPGLDAAVTGASRVLWP
                     PGLAGTRLFEFGTGRPWLCAQLLEQLATGIGAPPPREGQH"
     gene            4811..5008
                     /locus_tag="PLW13_04910"
     CDS             4811..5008
                     /locus_tag="PLW13_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HQY69742.1"
                     /translation="MERGIVGFHQDEEGHWVAELECGHMQHVRHDPPWVNRPWVTSAE
                     GRAGMLGFMLNCRLCDAPRES"
     gene            5126..5812
                     /gene="aqpZ"
                     /locus_tag="PLW13_04915"
     CDS             5126..5812
                     /gene="aqpZ"
                     /locus_tag="PLW13_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQY69743.1"
                     /translation="MKKYAAECFGTFWLVLGGCGSAVLAAAFPDVGIGLLGVSLAFGL
                     TVLTMAVAIGHISGCHLNPAVSVGLFVGGRFPANQLLPYIAAQVVGGIVAGGVLYLIA
                     SGKAGFDVAGGFASNGFGEHSPGGYSMVSALVTEVVMTMMFLLVILGATDKRAPAGMA
                     PIAIGLCLTLIHLISIPVTNTSVNPARSTGVAVFVGDWAVAQLWLFWVAPIVGAVLGA
                     LAYRFIGSED"
     gene            6056..7243
                     /locus_tag="PLW13_04920"
     CDS             6056..7243
                     /locus_tag="PLW13_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQY69744.1"
                     /translation="MNFEEYLRILARNDGSDLYLSTGAPPCAKFQGALKPLATEPMKP
                     GEIAGIAHALLDDEQRQQFAQELEMNLAVSIPGAGRFRINIFRQRNEVSIVARNITTE
                     IPKFDDLRLPPVLKEVVNAKRGLILFVGGTGSGKSTSLAALIDHRNSTSGGHIITIED
                     PIEYVHRHKKCIVNQREVGVDTRSFHNALVNTLRQAPDVILIGEVRNRETMEHALAFA
                     ETGHLAISTLHANNANQALDRIVNLFPEERRPQLLMNLGQNLRAFVSQRLVPTVDGKR
                     CAAVEVLLGTKTIEELIIKGEFESIKEIMSKSEALGMQTFDAALYNLYKEGRISMDEA
                     IRNADSQNDVRLRIKLDQSGGSLALEERSTSDGRLETRTSTGLTLSLEEMEEKGKDEK
                     PGH"
     gene            7252..9054
                     /locus_tag="PLW13_04925"
     CDS             7252..9054
                     /locus_tag="PLW13_04925"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQY69745.1"
                     /translation="MGEDRIEARDIARAALGLIPETPRLLRAALSLARLHPEAENSIG
                     LLLERRARETPWHTALMFEGECWSYHAFNAWANRIARVMRATGVRRGDTVAILCENRP
                     SMLACVAAVAKIGAVAGMLNPNQRGAVLRHSIELLAPRVFVVADDCVAALRSALPRSP
                     RGVKLLWDGAGAPPRGMKDLRALAAGERATDPASTRRVKLADPFCFIFTSGTTGLPKA
                     AVMTQLRWMRVGYAMGQGAMRLGADDVFYCALPFHHNSALTVCWSAVLTAGATLVMAR
                     KFSASRFWDDIRANGATAFVYIGEFCRYLLALPPSPDDRRHAVRVMVGNGLRPEIWDE
                     FQHRFGVAHVCEFYGSSEGSLGFVNALGLPRTAGVCPLPYAIVAFDAEREEPLRDARG
                     RLRRVSPGETGLLLMPVGRLMPFDGYTDAAATGARLLHDVFRRGDTWFDSGDLVRDQG
                     FRHIAFVDRVGDTFRWKGENVATTEVERALAKVPGVAGATVYGVPVPLADGRAGMAAI
                     VPEPGRRFDGKAVAAVLARELPAYAVPRFVRLVTSHETTATFKVRKVELKQQGFDPGL
                     VADPLYLLVERARGYQKLTPRLHARIRSGQLRVP"
     gene            9132..10646
                     /locus_tag="PLW13_04930"
     CDS             9132..10646
                     /locus_tag="PLW13_04930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69746.1"
                     /translation="MQQLSGQDAMFLYGEMDNTPMHIGPVFIYDPSTAPGGLVRFKDV
                     LRTFGERIHRSPVFTRRLSMVPFNLDHPFWVDGGETDIEFHVRHIALPKPGDWRQFCI
                     QIARLHARALDRARPLWEAYIIEGLDNVEGLPPGSFAMFLKIHHAAIDGATGVELMGA
                     LHDLEPVAPPSGRAGHRRTGATNAGNPDIEMLRRVAGGLIRQPLGVLEMIGKSLPAWQ
                     RVREGKREQRFRSLGEKERTRFNARVSAHRVFGAVNFDLREVVAVKSSVEGATVNDVM
                     LAIVSGAMRSYLQAKGELPHGSLVTGAPVNVRADAERGTGGNLVSMMSIALRTDVADP
                     LGRLRQVHEAAVNSKAYMNAVGARLLTDWTNRLPAQVTALGFRAAAAAGLLSAGKPVF
                     NTIVTNVPGPQVPLYFAGARLVRSFGAGPCVDGMGMFQVVTSYAGQIAISFQSCRDMM
                     PDPGFYERCLADSFAELKQAAASVSVRQPGRKPAPKRAKRIATRAATPRAGRKG"
     gene            10655..>11695
                     /locus_tag="PLW13_04935"
     CDS             10655..>11695
                     /locus_tag="PLW13_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HQY69747.1"
                     /translation="MRALVIERYGAPEEVLRLVEMPAPQPGPGEVRVRMRLSPVNPSD
                     FNTIRGGYQQSLERAIWNRGGGPLHFDPGRTRRLGALPVSPGADGMGVVESAGSGWYA
                     RRLVGRRVIVMGAPSGNWREQVVLPAMQAMPVPDGVSDEQAAMFLVNPMTAWAMVTQV
                     LKVPRGAWLLQSGGASQLARMVIRLGRRRGFRTISLVRRAGVAAELERLGADAVVDAS
                     REDVIARVAGLTGNAGVRHALDCVGADTLATMVRCLAPGAHLLCYGTLGGDMLHFPVR
                     DLMLPAARIEGFLLPQWIAGRGLAARLHAARTVGKLIAQGTLASEVGTVYPMVEYAVA
                     LRESLVPARGGKV"
     assembly_gap    11696..11795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11796..12337)
                     /locus_tag="PLW13_04940"
     CDS             complement(<11796..12337)
                     /locus_tag="PLW13_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HQY69748.1"
                     /translation="MSLSMYQASVPVLIHNLKALSAILKKGADHAKARGLDPTVLVNA
                     RLFPDMFPLVRQVQIATDMAKGGAARLAGVEIPSFPDTESSFPELQERLTRTIAFLKS
                     IKPAQLEGSEQREVNLQMRMGAVTFTGQYYLLSWVMPNVYFHVTTAYNILRHNGVELG
                     KWDFLGKAPGANIKAGKPAK"
     gene            12464..13225
                     /locus_tag="PLW13_04945"
     CDS             12464..13225
                     /locus_tag="PLW13_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69749.1"
                     /translation="MHGLEDKVVVVAGGGAIGSATVHRLADAGARVVVGDLNGEHAES
                     LADAVRESGGHCIGMGFDIADEASVRALVDAAVEEYGGIDAMHVNAADLQVVNQDSDV
                     LSEPLAVFDQTLRVSLRGHFLCTRAVLPELLRRGGGSIVYTSSVAAAVGEPVRPAYAM
                     AKAGIEALMRHVASGWGKQRIRANAVAPGLVITPAMATGLPEEARKHALKSVRSARLG
                     KPGDIASMVAFLVSDESEWINGQVYSVDGGTLLRL"
     gene            13237..14115
                     /locus_tag="PLW13_04950"
     CDS             13237..14115
                     /locus_tag="PLW13_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69750.1"
                     /translation="MSELLAAIIAGVPADFADPGADWRAVRAMMAPFHGQPCSPELAV
                     EIRACGGVATGFHTLDGVDGDGRIAFHCHGGAFVSCPLSDYHFYGEMIARALGMPVAM
                     PDYRLAPEHPFPAAHEDCFNAYRGLLESGVDPARICVLGESCGGSLALGALLRARDAG
                     LPMPACFVSVTGWFDLSVPGTPRGRDPFLTPQWVRNRAREYTAGALELTDPRISPARA
                     DLRGLPPLYLQVAQFDTMADGALALAANALHAGVEVTLESWPGLIHGWHGLANAGVPE
                     AAAAWASIRRYVDARM"
     gene            complement(14123..15043)
                     /locus_tag="PLW13_04955"
     CDS             complement(14123..15043)
                     /locus_tag="PLW13_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQY69751.1"
                     /translation="MNTPSPARTVRIAAAQYDVAPLADWAAYERRISAWVDEAALAGA
                     RLLLFPEYFAMELASLFGPEVPLSLPRQLSAMQELLADFLALFARLARTHGACIVAGS
                     YPVRLADGSYRNRCHLFHPDGRCMFQDKLQMTRFETEEWHIGAGDTLKVFDTAFGRIG
                     INICYDSEFPLLAHRQVAAGADLILVPSCTDTRAGYHRVRLGSRARALENQCFVVQSP
                     TVGDAPWSAAVDRNTGAAAVYAPVDYGFPDDGVLVEGELDRAQWVYADLDLGALAEVR
                     RYGQVCNFRDWDAQARIAGVEILAPDDTSG"
     gene            complement(15040..15630)
                     /locus_tag="PLW13_04960"
     CDS             complement(15040..15630)
                     /locus_tag="PLW13_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY69752.1"
                     /translation="MEIRTVAGEELVAAIPALARLRMTVFRDYPYLYDGSEAYETGYL
                     RTYASCDAAMAVLAREGDTIVGASTGIPMAHETAAFREPFARLGLDPARIFYCGESVL
                     LPAYRGRGIYRAFFRGREDWARTLGGFTQICFCAVQRPADHPLRPRDYVPLDAAWRHF
                     GYAPEPGLTMQLAWKDIDRDADTEHTMRFWFKALSA"
     gene            complement(15632..16033)
                     /locus_tag="PLW13_04965"
     CDS             complement(15632..16033)
                     /locus_tag="PLW13_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HQY69753.1"
                     /translation="MSAAALITRYYGAFNAGDMDAMLGLLHEDVAHDINQGRREVGRH
                     AFGHFMERMNRCYREQLSDIVVMANDEGTRAAAEFVVHGEYLASDAGLPEAKGQRYVL
                     RAGAFFEIREAQIARVTNYYNLEHWLQQVRS"
     gene            complement(16036..16584)
                     /locus_tag="PLW13_04970"
     CDS             complement(16036..16584)
                     /locus_tag="PLW13_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY69754.1"
                     /translation="MSHAYPPELQELIDKQAIRDLLAAYFTAADRHDNVKMAALYHDD
                     ATDDHGSFFKGLAKDFIATLPKIQEGMAILHHNMTTANIRIEGSYAEGEIYVLAFHQV
                     KKEDGGKLDLLIGGRYFDKYEKRDGVWKFLHKAVVADWATLHDPSIVNLDNPIIEGSY
                     IGKPGAADPSYGVFRLIRRGER"
     gene            16741..18192
                     /locus_tag="PLW13_04975"
     CDS             16741..18192
                     /locus_tag="PLW13_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY69755.1"
                     /translation="MRRLALIDAFFLMNETRQTPMHVGGVNLFTLPEGADEQEFLSHL
                     GNILRHEGPLRRPFGERLKMGPLGVAGPLYWEPDTQLDMDYHVRHSALPKPGRYRELF
                     ALVSRLHGTLLDRNRPLWEMHLIEGLQKRQFATYMKAHHCAIDGIGAMHLTQSMMSSR
                     PRTRIRYSPFSVEACEAYRKKLGATKSRVHEPEAHELRAVAEVLKQQFDSSRNIAAAL
                     GRYANVWFGRGGDLAVPWHHIPRTPFNTPVSGSRRFVAQSWPFERVRAVGKAFDGTLN
                     DAVLAMCSGALRRHLLDQDALPRESLKAMAPVSLRAEGDVDSANAVGFFTADLATQVA
                     DPAERVRAIQRSMQAAKQQFAGMTREEIALYTVLTNAPLLLTTVLGLASRFPAFSTVV
                     SNVPGPRQTMYWNGARLDGIYPASIPFHGFAMNITLVSYADKLDFGIVACRRSVPGVQ
                     RIIDHMEEALVELEEAAGLRRARRGVRHARRAG"
     gene            18229..20220
                     /locus_tag="PLW13_04980"
     CDS             18229..20220
                     /locus_tag="PLW13_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HQY69756.1"
                     /translation="MPRGSLAAASLLAALLAGCTDQAPPPAPGADAQGHSAPTAATIS
                     VNRAAAKDLALDDTRDFEDARRGLIASVPELVVAGSGATPIWDMPGYAFIEGAAPDSV
                     NPSLWRQAKLNNIHGLFQVSEGIYQLRGFDLSNMTLIEGEQGWIVVDPLTSRETAARA
                     LAFAREHLGDRPVSAIIYTHSHVDHFGGVLGVASAEEIAARGIRVIAPEGFMEEATSE
                     NMLAGTTMGRRAAYMYGQHLAHAPRGHVDTGLGKGPAFGSAGIVAPTETVGRTGEELV
                     IDGVRFVFQYTPGSEAPAEFTFYLPDLKAFCGAEIVSHTLHNLYTLRGAKVRDALKWS
                     GYIDEAIRLFGDAEVYFGSHHWPLWGEARITDFLQKQRDTYRYIHDQTLRLAALGRTP
                     QQIADELELPTSLAREFANRGYYGTVRHNAKAVYQFYFGWYDANPSRLNPLAPAEAAS
                     RYVEFMGGGAAVLAKAQASFDKGDYRWVAEVLTHLVFAEPDNGAARELLARAYDQLGY
                     QAESGPWRDAYLSGAWELRHGVPQEAGDVGAVLELLRNTPIERFLDTMAVRLDGAKAA
                     ERHTVINLVLSDTGDSHVLELANGVLHHWREPPRAQADATITVTHALFIDLLTGRAGL
                     RETLGSDALEVDGSRVELLRFFALFEQPDTAFPIVTPAG"
BASE COUNT         3139 a         6862 c         7321 g         3244 t
ORIGIN      
        1 cttgttgttc tcgcgcagct tgctcaccag cggcgagaag tcggagtcgc cgctgatgat
       61 cacgaacatg tcgacgtgcg acttggtgta gcagaggtcg agcgcatcca ccaccatgcg
      121 gatgtccgcg gagttcttgc ccgactggcg cacgtgcggg atctcgatca gctcgaagga
      181 ggcttcgtgc atcgccttct tgaactcctt gtaccgctcc cagtcgcagt aggctttctt
      241 gaccacgatg ttgcccttca gcaacagccg ttccagcacc ttgcggatgt cgaaggcggc
      301 gtacttggca tcgcgcacgc cgagcgccac gttctcgaag tcgcagaaca gcgccatgtt
      361 acgggtattg ggtctgtcac tcatgcggtt gtcctagtcc agtccaaaga aggcgcgaat
      421 gcgggccagc atatcggtac gcgcgcttgc cgccgcgtgt gctccggcgg ccgagccgcg
      481 cagctcgccc gtgagcatgc cgcgctcacg gaagatatcc cccggcccga gcgaactcac
      541 gtaacggcgc gtgccgccgg tctcgtcgca gatctcggcg gcgccctcga tcacgaggta
      601 cagccagtgt gccacggcgc cctggcgcgt caccacgtca cccgcgagaa agggcgcgtg
      661 caccaggtgc tgcgcaacct gcgccctttc gtcgtccgac agcgagccga acagcggtac
      721 cattcgcagg gtattcatgc gtcgcgcgat ctccgcggcg gccagcgccg cgcggcgcgc
      781 ctcgtcctcg gcgaacacca ggcgctcctc gcgacgcgaa gcgaggtgga tgccggcgcg
      841 atccagggcg gccagcgcat gggcgcgcac ccgcgagtcg gtggcgtcgt ccacttgggg
      901 gtcgcgcagc cagtagcgca gcgcatagcg ggcgcagccg tcggtgacat ccaccaggat
      961 cgcatccggc ttccggatcg cggctcacgc gcgaggcgcg gatagtcagg cgcgatcggg
     1021 ctggcggcga gcagttcggc gcccgcgcac gtcagcagga gcacgagcgc gttgcgcacg
     1081 ctggcgcgcc ctgcgcgctg ggtcgcgagc aatatgccgc ccagcgccag tcctgccagg
     1141 acggcggttg cgagttgcag gcggaatgtc agatccatcg cggcaggcag cccagtgttt
     1201 tcccggcaca ccgccagtac gttaacccat cgccggcggc tgcggatacc gcaccgcgcc
     1261 tgcagggggg ggcgcgagtg ttgccgccgg atggcatcgg cgtatattag ccgcgagggc
     1321 tcgcgggccc ggcgcgcggt ggtggaggct cacaggagga acatccatgc tgaagcaggt
     1381 cgaactgaag cactacatgg tgcagaatcc cgtcaaggtg cgcgccgacg cgaacctcct
     1441 cgatgccatc cacctgatcc tcgtgaacaa gatatcgggc ctgtgcgtgg tcgatgagcg
     1501 caacaagctg atcggcgtgc tgtccgagac cgactgcctg cgcggcgtgc tgagcgcgac
     1561 ctacaacgac tcgggcatcg gcagcgtggg cgaattcatg agttcgcaga acctgcacac
     1621 cgcgtcgccg ggcgatcaca tcgtcgatat tgccgccgac atgctcaagc acaagatacg
     1681 gcgccgcccg gtgatcgaca gcgacggcac gctgctcggc cagatcacca ttcgccagat
     1741 cctgcgcgcg gtgaaggaat tctccagccc gaaggatcgc accgaacgcg actgagcgcg
     1801 tgtgcggccg atgcgccatg acagtgtctg gccaatcccg cagagatgct gtccctggtg
     1861 atgcgcgaga acctcaacat gcggcaccac gatctcgagg ggctgcagta tcaggagatt
     1921 ttgccgccat gcgcacgaaa atcccgaagt cattcgcagt ggcgctcatc ctcagcgccg
     1981 gcgtgcaggc gcaggaactg cccgaggcgg tcccgatgcc gtccgccgcc cttccctcgg
     2041 tcgaattgcc cacggtcgca ttgccgcccg ggcttgatcg cgtgctgcgc gactacgagc
     2101 tggcttggat gcagaagcaa ccgtcggctc ttgccgggat gttcacaatg gacggcatgg
     2161 cattgcatag cggcagccca cccgcgcgcg gcgccgcccg gatatcggca gggtatgccg
     2221 aagccgcggg caccccgctg tcgctgcgcg cgctggcgta cgcggtctca catgacatgg
     2281 cgtatatcgt cggcgctttc gcgcccgctg ccggcgaacc ggaccacggc aagttcgtgc
     2341 tggtcctgcg ccgcgggagc gatggcgact ggaagattgc cgccgacatc gacaacatga
     2401 acgccctgcc gggagcgccg acgccggagc cggagcaatc gaatgcgccg gtatccgtgc
     2461 cggagcccta ggcccgcgtc ctggtcgtcg agcgcgaagg cggcatctga ggcagaggcg
     2521 cgaccatgct tcatcacatt tcctttggcg tgcgcgacct ggccttgtcc ggcgcgttct
     2581 acgacgccgc gctcggtgcg ctgggctttc gccgcgtatt cgaggcggga aatgccatcg
     2641 gttacggcct gctcgatggc gaggacgtgc tttacctgca gcagcggccg gatgccgcgg
     2701 cgccgggccc cggctttcac ctggcgttcg ccgcaccgtc gcgcgaggcc gtggacgctt
     2761 tccatgcatg cgcattgcgc gccgggggca cggacaacgg cgcgcctggc cggcgcccgc
     2821 actacgggcc ccactactac gcggcgttcc tggtcgatcc ggacgggcac cggatcgagg
     2881 cggtctgcaa ggctgccggg taagcgctcg cggcaaactg aggctgcccg gcggcggtga
     2941 cgcctgatgc cggttgatga ccaggagcga aacccatgac ggatcccttc cttcttgcag
     3001 cgatagagga agcggaatgc ggcctcggcg agggcggcat accgatcgga tcggtgctgg
     3061 tccacgacgg ggccatcgtc gggcgcgggc acaatcgccg cgtccagcag ggcagcacca
     3121 tcctgcacgc cgagatggat gcgctcgaga acgccggccg ccgcgacgcc gcgttctacc
     3181 gcgagtgcac gctgtacacg acgctctcgc cctgcgccat gtgcagcggc gcggtcgttc
     3241 tttacggcat tccccggcta gtcgtgggcg agaaccggtc gtacatgggc gaggagcagt
     3301 ggctggcgag ccgtggcgtg cggatccggg tgatccagga cgcgcggtgc atcgcgctga
     3361 tgcgcgagtt catgcagcgg catccggggc tgtgggccga ggatatcggc gagtgaacag
     3421 ggagcggcga cgcgctgccg tggagcattg acccatgctg acactgaagc agctcgagac
     3481 ctacgaagcc tgcggtggcg acgccgacgg ctggacgcgc cgtggcggtt cgcccggcat
     3541 ggactatgag cactggctgt tgatcgacga gctgctgcaa ggactgcatc tgatacgatc
     3601 cggcctggct tcgcaacgct acgcggaccg catccgcggc gcgctgctcg ccgtcacgga
     3661 cgatgaggca gtgcgccaca agctcgcgca aatgagtgga gacatgacat gaaagcaggg
     3721 ccaggcatac ttttcgccgt atcgctgctg ctcggcgcgt gcagcagcgc ttactacggc
     3781 accatggaaa agctcggcgt gcacaagcgc gacatcatgg tcgatcgcgt ggtggcggcg
     3841 cgcgatgccc agaccgaggc caaggagcag ttcctcaccg cgatggagca gttcaggggc
     3901 gtggtcgcct tccagggggg cgacctggag cgcgagtacc acaagctgga tgcgacgctg
     3961 caggagagcg aagcgcgggc cgccgcggta cgtgagcgca tcagcgcggt cgaggacgtg
     4021 tccggggcgc tgttcagcga gtgggaggcg gagttggggc agtactccag cgatacgctg
     4081 cgccaggcca gccagcgcaa gtacgacgcc acgaagcgca agtacacgca gctgctcgcg
     4141 gccatgaaga gggccgaggc gcggctggag ccggtgttgg tgccgctgcg cgaccaggtg
     4201 ctgttcatga agcacaacct caatgcgcgc gcgatcgcgg ggctgagcga ggaggtggtc
     4261 agcgtgcagg ccaacgtgga caggctggtg cgcgagatgg agagcgcgat cacgcaggcc
     4321 gacgcgttca tcgccgagct gcgggccgag gaatgagcgc ggcgtcggcg cgggtgtgcg
     4381 catgagcgtc tgcctcgcgg cctcgctcgc gctgcagggc ggcgcgctgg tctatgcggg
     4441 gcccggcggc gctgcctggc gcgtggacgc cgacagcatc cgcgcagtcg gggagctgac
     4501 cacggcgggc tgcggcgagg atcacctgct ggccttcgtg gtcgatgccg gcgggggctg
     4561 gttccaggcg tcgcgcggcg ccctcggcgc cgacgaggcg ctcgcggcgc tcgggctcag
     4621 gctcggcggg gcgatcgttc cggggctgga cgccgcggtc actggcgcga gccgcgtgtt
     4681 gtggccgccc gggctcgcgg gcacacgcct gttcgaattc gggaccggca ggccttggct
     4741 gtgcgcgcag ctgctcgaac agctggctac cggcattggc gcgccgccgc cgagggaagg
     4801 gcagcactga atggaacggg gcatcgtggg cttccaccag gacgaggaag ggcactgggt
     4861 ggccgaactg gaatgcgggc acatgcagca cgtgcgtcat gacccgccgt gggtgaaccg
     4921 cccctgggtg acgagcgccg agggccgcgc gggcatgctg ggcttcatgc tcaactgccg
     4981 cctgtgcgat gcgccccggg agtcgtgatc gcgcgccttt gacggggtgc cccgaagcgg
     5041 accagaatgc ggtgcggcgg atcggctgcg atcccgtgcc gaccgctggt ggattaaaag
     5101 aatcgacaac agcagggaga agcgcatgaa aaagtatgct gcagaatgct ttgggacgtt
     5161 ctggctggtc ctgggtggtt gcggaagcgc ggtgctcgcc gcggcgtttc cggatgtcgg
     5221 catagggctg ctcggagtat cgctcgcctt cggtctgacg gtcctcacga tggcggttgc
     5281 gatcgggcac atctccggtt gtcacctgaa tccggccgta tcggtcgggc tcttcgtggg
     5341 cggacgcttt ccggcgaacc agttgctgcc ctacatcgcc gcacaggtcg tggggggcat
     5401 cgtcgccggt ggcgtgctgt acctgatcgc cagcggcaag gctggcttcg atgtcgcggg
     5461 cgggtttgca tccaacggct tcggcgagca ttcgccgggt ggctactcga tggtctctgc
     5521 gctcgtcacc gaggtggtga tgacgatgat gttcctgctg gtgatcctcg gggccaccga
     5581 caagcgcgcg ccggcgggca tggcaccgat cgcgatcggc ctgtgcctga cgctgatcca
     5641 cctgatcagc attcccgtca ccaacacctc ggtgaacccg gcgcgcagca ccggggtcgc
     5701 ggtgttcgtc ggtgactggg cggtggcgca gctgtggctg ttctgggtcg caccgatcgt
     5761 cggtgcggtg ctcggtgcgc tggcctaccg cttcatcggc agcgaggatt gacgccgcgc
     5821 gcaccgggcc gcatgcgccg cggcccggtg cgcaacacgg cgcatcgtcc atctctccag
     5881 gttctcgcat cacaggcgat cgggcctcac gctgggcgcg ctgctgcagt gtgacggcta
     5941 tcacgcgcgc cgcaagcgaa gcccggtgcg gcccgcggca ggcgaacatc gcgcggcgcg
     6001 agcacggata aatgcctatg cttgctgcac cttgcctggc tggagagcca acgccgtgaa
     6061 tttcgaggaa tacctgcgga tcctggccag gaacgacggt tccgacctct atctcagcac
     6121 cggcgcgccg ccgtgcgcga agttccaggg cgcgctgaag ccgctggcga ccgagccgat
     6181 gaagccgggc gagatcgccg ggatcgccca cgccctcctc gacgacgagc agcgccagca
     6241 gttcgcgcag gaactggaaa tgaacctcgc ggtctcgatc cccggcgccg ggcgctttcg
     6301 catcaacatc ttccgccagc gcaacgaggt ctcgatcgtc gcgcgcaaca tcaccaccga
     6361 gatcccgaag ttcgatgacc tgcgcctgcc tccggtgctg aaggaagtgg tcaacgccaa
     6421 gcgcggcctg atcctgttcg tcggcggcac gggttcgggc aagtcgacct cgctcgcggc
     6481 gttgatcgac caccgcaaca gcaccagcgg cggccatatc atcaccatcg aggatccgat
     6541 cgagtacgtg caccgccaca agaagtgcat cgtgaaccag cgcgaggtgg gcgtggacac
     6601 gcgcagcttc cacaacgcgc tggtgaacac gctgcgccag gcgcccgacg tgatcctgat
     6661 cggcgaggtg cgcaaccgcg agaccatgga gcatgcgctc gccttcgccg agaccggcca
     6721 cctggcgatc tccacgctgc acgccaacaa cgccaaccag gcgctggacc gcatcgtgaa
     6781 tctcttcccc gaggagcgcc gcccgcagct gctgatgaac ctcggacaga acctgcgtgc
     6841 cttcgtttcg cagcggctcg tccccaccgt cgacggcaag cgctgcgcgg cggtcgaggt
     6901 gctgctcggc accaagacca tcgaggagtt gatcatcaag ggcgagttcg aatcgatcaa
     6961 ggaaatcatg tcgaaatccg aggcgctcgg catgcagacc ttcgacgccg cgctctacaa
     7021 cctctacaag gaagggcgga tctcgatgga cgaggcgatc cgcaacgcgg actcgcagaa
     7081 cgacgtgcgc ctgcgcatca agctggacca gtccggcggc agcctggcac tggaggagag
     7141 atcgacctcc gacggcagac tggagacgcg gacctcgacg ggcttgacgc tgtcgctgga
     7201 ggaaatggag gagaagggca aggacgagaa gcccggtcac tgaggggcac gatgggcgaa
     7261 gaccgcatcg aggcacgtga catcgcgcgc gcggcgctcg ggttgatccc cgaaacgccg
     7321 cggctgctgc gcgccgcgct cagcctggct cggttgcacc cggaggcgga aaactcgatc
     7381 ggactgttgc tggagcggcg cgcgcgcgag acgccgtggc acacggcgct gatgttcgag
     7441 ggcgagtgct ggagctacca cgcgttcaac gcctgggcca accgcatcgc gcgcgtgatg
     7501 cgcgctaccg gcgttcgccg cggtgatacc gtcgccatcc tctgcgaaaa ccgcccttcg
     7561 atgcttgcgt gcgtggcggc ggtcgccaag atcggcgcgg tcgccggcat gctgaacccg
     7621 aaccagcgcg gcgcggtgct gcgccacagc atcgagctgc tcgccccgcg ggtattcgtc
     7681 gtggccgacg attgcgtggc ggcgctgcgc agcgcgctgc cgcgcagccc gcgcggcgtg
     7741 aagctactgt gggacggcgc cggcgcgcca ccgcgcggca tgaaggacct gcgcgcgctg
     7801 gcggccggcg agcgggccac cgatccggcg agcacgcgac gcgtgaagct cgccgatccc
     7861 ttctgcttca tcttcacgtc gggcactacc gggctgccga aggcggcggt gatgacgcag
     7921 ctgcgctgga tgcgcgtcgg ctacgcgatg gggcagggcg cgatgcgcct cggcgccgac
     7981 gacgtctttt actgtgcgct gccttttcac cacaacagcg cgctcacggt gtgctggagc
     8041 gcggtgctca cggccggcgc cacgctggtg atggcacgca agttcagcgc ctcgcgcttc
     8101 tgggacgata tccgcgcgaa cggcgccacg gccttcgtct acatcggcga gttctgtcgc
     8161 tacctgctcg cgctaccgcc ttcgcccgac gaccgtcgcc acgcggtgcg cgtgatggtc
     8221 ggcaacggcc tcaggccgga gatctgggac gagttccagc accgcttcgg cgtcgcgcac
     8281 gtctgcgaat tctacggttc cagcgagggc agcctcggat tcgtcaacgc gctgggcctg
     8341 ccgcgcaccg ccggtgtctg cccgctgccc tatgcgatcg tggcctttga cgccgagcgc
     8401 gaggagccgc tgcgcgatgc gcgcgggcgc ctgcggcgcg tgtcgcccgg cgagaccggg
     8461 ctgctgctga tgccggtcgg gcggctcatg cccttcgacg gctacaccga cgccgccgct
     8521 accggcgcgc ggctgctgca cgacgtgttc cgccgcggcg acacgtggtt cgacagcggc
     8581 gacctggtgc gtgatcaggg ctttcgccac atcgccttcg tcgatcgcgt cggcgacacc
     8641 ttccgctgga agggcgagaa cgtggcgacc accgaggtcg agcgcgcgct cgcgaaggtg
     8701 cccggcgtgg ccggggccac ggtctacggc gtgccggtgc cccttgccga cggtcgtgca
     8761 ggcatggccg cgatcgtgcc ggagcccggg cggcgcttcg atgggaaagc cgtcgcggcg
     8821 gtgctcgcgc gcgagctgcc ggcctacgcg gtgccgcgct tcgtgcgcct ggtgacgtcg
     8881 cacgagacca ccgccacctt caaggtgcgc aaggtcgagc tgaagcagca gggcttcgat
     8941 cccggcctcg tggcggatcc cctctatcta ttggtcgagc gcgcgcgcgg ctaccagaag
     9001 ctgacgccgc gcctgcacgc gcgcatccgc tcgggccagc tgcgcgtgcc ctgagccggc
     9061 gcgggaaacc cgcggtgcat ggcagagttg cgctacagta gctcccgaca acggcccggg
     9121 aggagaccag catgcagcag ctcagcggac aggacgcgat gttcctctac ggcgagatgg
     9181 acaacacccc gatgcacatc ggcccggtgt tcatctacga cccgtccacg gcacccggcg
     9241 gcctggtgcg cttcaaggac gtgctgcgca cgttcggcga gcgcatccac cgctccccgg
     9301 tcttcacgcg ccgcctgtcg atggtgccgt tcaacctgga ccacccgttc tgggtcgacg
     9361 ggggcgagac cgacatcgag ttccacgtaa ggcacatcgc gctgcccaag cccggcgact
     9421 ggcgccagtt ctgcatccag atcgcccgcc tgcacgcgcg cgcgctcgat cgcgcgcggc
     9481 cgctgtggga ggcctacatc atcgaggggc tggacaacgt ggaggggctg ccgccgggca
     9541 gtttcgcgat gttcctgaag atccaccacg cggcgataga cggcgccacg ggcgtcgagc
     9601 tcatgggcgc gctgcacgat ctggagccgg tggcgccgcc gtcggggcgc gccgggcacc
     9661 ggcgcaccgg cgcaacgaac gcgggaaacc ccgacatcga gatgctgcgc cgcgttgccg
     9721 gcgggctgat tcgccagccg ctcggcgtgc tggagatgat cggcaagtcg ctgccggcgt
     9781 ggcagcgcgt gcgcgagggc aagcgcgaac agcgctttcg ctcgctcggc gagaaggagc
     9841 gcacgcgctt caacgcccgt gtatcggcgc atcgcgtgtt cggcgcggtg aatttcgatc
     9901 tgcgcgaggt ggtcgcggtg aagagcagcg tcgagggcgc gaccgtgaac gacgtgatgc
     9961 tcgcgatcgt ctcgggggcg atgcgcagtt acctgcaggc gaagggtgaa ctgccccacg
    10021 gcagcctggt gaccggtgca cccgtgaacg tgcgcgccga cgccgagcgg ggcaccggcg
    10081 gcaacctggt gtcgatgatg agcatcgcgc tgcgcaccga cgtcgccgat ccgctcgggc
    10141 gcctgcgcca ggtgcacgag gcggccgtga actccaaggc ctacatgaac gccgtgggtg
    10201 cgcgcctgct gacggactgg accaaccgcc tgccggccca ggtgacggcg ctgggatttc
    10261 gcgccgccgc cgcggccggg ctgctgagcg ccggcaagcc ggtattcaac accatcgtca
    10321 ccaatgtgcc ggggccacag gtgccgctgt acttcgccgg cgcgcgcctg gtgcgcagtt
    10381 tcggcgccgg gccctgcgtg gacggcatgg gtatgttcca ggtcgtgacc agctacgccg
    10441 gccagatcgc gatctccttc cagtcctgcc gcgacatgat gcccgacccc ggtttctacg
    10501 agcgctgcct cgcggattcc tttgccgaac tgaagcaggc cgcggcgagc gtgtcagtac
    10561 gccagcccgg acgcaaaccc gcaccgaagc gcgcgaagcg catcgccacg cgtgccgcga
    10621 cgccgcgcgc cgggcgcaag ggctgagcat tgcgatgcgc gcccttgtga tcgagcgcta
    10681 cggcgcaccc gaggaggtgt tgcgcctcgt ggagatgccg gcgccgcagc ccggccccgg
    10741 tgaggtgcgc gtgcgcatgc gcctctcgcc ggtgaacccc tccgatttca acaccatccg
    10801 cggcggttac cagcagagcc tggagcgcgc gatctggaac cgcggcggcg gaccgctgca
    10861 tttcgacccg gggcgcacgc gccggctcgg tgcgctgccg gtgtcgccgg gcgccgacgg
    10921 catgggtgtg gtcgagtccg ccggcagcgg ctggtacgcg cggcggctcg tgggcaggcg
    10981 cgtgatcgtg atgggcgccc cgtcgggcaa ctggcgtgag caggtggtac tgcccgcgat
    11041 gcaggcgatg ccggtccccg atggggtgag cgacgagcag gccgcgatgt tcctggtcaa
    11101 cccgatgacg gcctgggcga tggtgacgca ggtgctgaag gtgccgcgcg gcgcctggct
    11161 gctgcagtcg ggcggtgcct cgcaactggc gcgcatggtg atccggctgg ggcgtcgccg
    11221 gggctttcgc accatcagcc tggtgcggcg tgccggtgtg gcggccgagc tcgaaagact
    11281 cggcgccgac gcggtggtcg acgcctcgcg cgaggacgtg atcgcgcgcg tggccgggct
    11341 caccggcaat gccggcgtgc gtcatgcgct cgactgcgtg ggcgccgaca cgttggcgac
    11401 gatggtgcgc tgcctggcgc cgggcgcgca cctgctgtgc tacggcacgc tcggcggcga
    11461 catgctgcat tttcccgtgc gcgacctgat gttgccggcg gcgcgcatcg agggcttcct
    11521 gctgccgcag tggatcgccg ggcgcggtct cgcggcgcga ttgcacgccg cgcgcacggt
    11581 gggcaagctg atcgcgcagg gcacgctggc ctccgaggtg ggcaccgtgt atccgatggt
    11641 ggagtacgca gtagcgctgc gcgagagtct cgtgcccgcg cgcggcggca aggtgnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttttt cgccggcttg cccgccttga
    11821 tgttcgcgcc cggcgccttg ccgaggaaat cccacttgcc cagttccacg ccgttgtggc
    11881 gcaggatgtt gtaggcggtg gtgacgtgga agtacacgtt cggcatcacc cagctcagca
    11941 ggtagtactg gccggtgaac gttaccgcgc ccatgcgcat ctgcaggttc acctcgcgct
    12001 gctcgctgcc ctcgagctgc gcgggcttga tgctcttcag gaaggcgatg gtcctggtga
    12061 ggcgctcctg cagttcgggg aaactcgatt cggtgtcggg gaagctcggg atctcgacgc
    12121 ccgcgaggcg cgcggcgccg cccttggcca tgtcggtggc gatctgcacc tggcgcacca
    12181 gcgggaacat gtcggggaac aggcgggcgt tcacgagcac cgtcgggtcg aggccgcgcg
    12241 ccttggcgtg gtcggcgcct ttcttgagga tcgccgaaag tgccttcagg ttgtggatca
    12301 ggacggggac cgacgcctgg tacatggaca gtgacatggt gaacgctccg ttggtgagtg
    12361 tggctggccg gacgcggggc atcttatgcc ctgcatcgcg ccgacgggaa gcccgacata
    12421 tactccgcgc tctatcacgc aatacgaaag cggagaacgg tacatgcacg gactcgagga
    12481 caaggtcgtg gtggtggcgg gcggcggcgc cataggctcc gccacggtgc atcggctggc
    12541 ggacgccggt gcgcgggtgg tggtgggtga cctgaacggc gagcacgccg agtcgcttgc
    12601 cgacgccgtg cgcgagagcg gcgggcactg catcggcatg ggcttcgaca tcgccgacga
    12661 agcctcggtg cgcgcgctgg tcgacgcggc cgtcgaggag tacggcggca tcgacgcgat
    12721 gcacgtgaac gccgccgacc tgcaggtggt caaccaggac tccgatgtgc tgagcgaacc
    12781 cctcgccgtg ttcgaccaga cgctgcgcgt ttcgctgcgc gggcacttcc tgtgcacgcg
    12841 cgcggtgctg ccggagctcc tgcgccgcgg cggcggttcg atcgtctaca cctcgtcggt
    12901 cgctgcggca gtcggcgagc cggtgcgccc tgcctacgcg atggcgaagg ccggcatcga
    12961 ggccctgatg cgccacgtgg cctctggctg gggcaagcag cgcatccgcg ccaacgcggt
    13021 ggcgccgggc ctcgtgatca cgccggccat ggccacgggc ctgcccgagg aggcgcgcaa
    13081 gcacgcgctg aaatcggtgc gcagcgcgcg cctgggcaag cccggcgata tcgcctcgat
    13141 ggtggccttc ctggtctccg acgagtcgga gtggatcaac ggccaggtgt actccgtcga
    13201 cggcggcacg ctgttgcggt tgtgagcggc gccgtcatga gcgagctgct ggccgctatc
    13261 atcgccggcg tgcccgccga tttcgccgac cctggcgcgg actggcgcgc ggtgcgcgcg
    13321 atgatggcgc cgttccacgg ccagccctgc agcccggagc tcgcggtcga gatccgcgct
    13381 tgcggcgggg tggcgacggg tttccacacg ctcgacggcg tggatggcga cgggcgcatc
    13441 gcgttccact gccacggcgg cgccttcgtc tcctgtccgc tgtcggacta tcacttctat
    13501 ggcgagatga tcgcgcgcgc gctcggcatg ccggtggcga tgcccgatta ccggctcgcc
    13561 ccggagcatc cgttcccggc cgcacacgag gactgtttca atgcctaccg cgggttgcta
    13621 gagagcggcg tcgatccggc gagaatctgc gtgctcggtg aatcctgcgg cggctcgctg
    13681 gcgctagggg cgttgctgcg cgctcgcgat gcgggcctgc cgatgccggc ctgtttcgtg
    13741 tcggtcacgg gctggttcga cctgtcggtg cccggcacgc cgcgtgggcg tgatcccttc
    13801 ctcacgccgc agtgggtgcg caaccgtgcg cgcgaataca ccgccggggc gctcgagctg
    13861 acggatccgc gcatctcgcc cgcgcgcgcg gacctgcgcg gcctgccgcc gctgtacctg
    13921 caggtggccc agttcgacac catggccgac ggcgcgctcg cgctggcggc caatgcgctg
    13981 cacgcggggg tggaggtcac gctggaatcg tggcccggcc tgatccacgg ctggcacggg
    14041 ctggcgaatg ccggcgtgcc cgaggcagcg gccgcgtggg cgtcgatccg ccgctacgtg
    14101 gacgcacgaa tgtagtccgg attcatccgg acgtgtcgtc cggcgcgagg atctccacgc
    14161 cggcgatgcg cgcctgcgcg tcccagtcgc ggaaattgca cacctggccg taccggcgca
    14221 cctcggcgag cgccccgagg tcgagatcgg cgtagaccca ctgcgcgcga tccagctcgc
    14281 cttcgacgag cacgccgtcg tcgggaaagc cgtagtcgac cggcgcatac acggcggcgg
    14341 cgccggtgtt gcgatccacc gccgccgacc atggcgcgtc gccgacggtg ggcgactgca
    14401 ccacgaagca ctggttctcg agcgcgcggg cgcggctgcc gaggcgcacg cggtggtagc
    14461 cggcacgcgt gtcggtgcag ctcggcacca ggatcaggtc cgcgcccgcg gcgacctggc
    14521 ggtgcgcgag cagcgggaac tccgagtcgt agcagatgtt gatgccgatg cgcccgaacg
    14581 cggtgtcgaa gaccttcagc gtgtcgccgg cgccgatgtg ccattcctcg gtttcgaagc
    14641 gcgtcatctg cagcttgtcc tggaacatgc agcgcccgtc ggggtggaac aggtggcagc
    14701 ggttgcggta gctgccgtcg gcgagccgca ccgggtagct gccggccacg atgcaggcgc
    14761 cgtgcgtgcg ggccagccgc gcgaacagcg cgagaaaatc cgccagcaac tcctgcatgg
    14821 cgctcagctg ccgcggcagc gacagcggca cctcggggcc gaacagcgag gccagctcca
    14881 tcgcgaagta ttcggggaac agcagcagcc tcgccccggc gagcgcggcc tcatcgaccc
    14941 aggccgagat ccggcgctcg tacgcggccc agtccgcgag cggcgcaacg tcgtattgcg
    15001 cggcggcgat gcgcaccgtg cgggcgggcg atggcgtgtt cacgcggaca gcgccttgaa
    15061 ccagaagcgc atcgtgtgct ccgtgtcagc gtcccggtcg atgtccttcc acgccagttg
    15121 catcgtcagc ccgggctccg gggcgtagcc gaagtggcgc cacgcggcgt cgagcggcac
    15181 gtaatcgcgc ggtcgcagcg gatgatccgc ggggcgctgc acggcgcaga agcagatctg
    15241 cgtgaagccg ccgagcgtcc gcgcccagtc ctcgcgcccg cggaagaacg cgcggtagat
    15301 gccgcggccg cggtatgccg gcagcagtac cgactcgccg cagtagaaga tgcgcgcggg
    15361 atccaggccg agacgcgcga aaggctcgcg gaacgccgcg gtctcgtgcg ccatcgggat
    15421 gccggtcgag gcgccgacga tggtgtcgcc ctcgcgtgcc agcaccgcca tcgccgcgtc
    15481 gcagctcgca taggtgcgca ggtagccggt ctcgtaggcc tcggagccgt cgtagaggta
    15541 gggatagtcg cggaacacgg tcatgcgcag gcgcgccagc gccgggatcg cagcgacgag
    15601 ttcctcgccc gcgacggtgc ggatctccat ctcaggaccg cacctgctgc agccagtgct
    15661 cgagattgta gtagttggtc acgcgcgcga tctgggcctc gcggatctcg aagaaggcgc
    15721 cggcgcgcag cacgtagcgc tggcccttcg cttcgggcag gccggcgtcg ctggcgaggt
    15781 actcgccgtg cacgacgaac tcggccgcgg cgcgcgtgcc ctcgtcattc gccatgacca
    15841 cgatgtcgct gagctgctcg cggtagcagc ggttcatgcg ctccatgaag tgcccgaacg
    15901 cgtggcgccc gacttcgcgg cggccctggt tgatgtcgtg tgcgacatcc tcgtgcagca
    15961 ggccgagcat tgcatccatg tcgccggcgt tgaaggcgcc gtagtagcgg gtgatcagtg
    16021 cggcagcgct catgctcagc gctccccgcg ccggatcagg cggaacacgc cgtaggaggg
    16081 gtcggctgcg ccgggcttgc cgatgtagga gccctcgatg atcgggttgt cgaggttcac
    16141 gatcgaggga tcgtgcagcg tggcccagtc ggcgaccacg gccttgtgca ggaacttcca
    16201 cacgccgtcg cgcttttcgt acttgtcgaa atagcgcccg ccgatcagca ggtcgagctt
    16261 gcctccgtcc tccttcttaa cctggtggaa ggccagcacg tagatctcgc cctcggcata
    16321 gctgccctcg atcctgatgt tcgcggtggt catgttgtgg tgcaggatcg ccatgccttc
    16381 ctggatcttc ggcagcgtgg cgatgaagtc cttcgctagg cccttgaaga aggaaccgtg
    16441 gtcgtcggtg gcgtcgtcgt ggtacagcgc ggccatcttc acgttgtcgt ggcggtcggc
    16501 ggcggtgaag taggcggcca gcaggtcgcg gatggcctgc ttgtcgatca gctcctgcag
    16561 ttcggggggg taggcgtggc tcatgcgggt actcgcgttg gcgtgaggga cgcggcgatt
    16621 atgcccaagc atcgcagggc tggcaccttt cctgcgctgg atcagggtac cgcttgggtc
    16681 ggtgacgctg cacggtgcta acatcggcgt ccggacgatt ccaataacac gggaggcccg
    16741 atgcgcagac tggccctgat cgatgcgttc ttcctgatga acgagacccg ccagacgccg
    16801 atgcacgtgg gcggggtcaa cctgttcacg ctgcccgagg gcgccgacga gcaggagttc
    16861 ctgtcgcacc tcggcaacat cctgcgccac gagggcccgc tgcgtcggcc cttcggcgag
    16921 cgcctgaaga tgggcccgct cggtgtggcc ggcccgctct actgggagcc cgacacccag
    16981 ctcgacatgg attaccacgt gcgccactcg gcgctgccca agcccgggcg ctaccgcgag
    17041 ctgttcgcgc tggtgtcgcg gctgcacggc acgttgctag accgcaaccg gccgctgtgg
    17101 gagatgcacc tgatcgaggg gctgcagaag cgccagttcg ccacctacat gaaggcgcac
    17161 cactgcgcga tcgacggcat cggcgcgatg cacctcacgc agagcatgat gtcgagccgt
    17221 ccgcgcacgc gcatccgcta ttcgccgttc tcggtggagg cctgcgaggc ttaccgcaag
    17281 aagctgggcg ccacgaagtc gcgggtgcac gagcccgaag cgcacgagct gcgcgcggtg
    17341 gccgaggtgc tcaagcagca gttcgacagc agccggaaca tcgccgcggc gctggggcgc
    17401 tatgcgaacg tgtggttcgg ccgcggcggc gatctcgcgg tgccctggca ccacatcccg
    17461 cgcaccccgt tcaacacccc ggtgagcggg tcgcgccgct tcgtggcgca gtcctggcct
    17521 ttcgagcgcg tgcgcgccgt tggcaaggcc ttcgacggca ccctgaacga cgcggtactg
    17581 gcgatgtgct ccggcgcgct gcgccgccac ctgctcgacc aggacgcgct gcccagggag
    17641 tcgctgaagg cgatggcgcc ggtgtccttg cgcgccgagg gcgacgtgga ctcggccaat
    17701 gccgtgggct tcttcacggc cgatctcgcc acccaggtcg cggacccggc cgagcgcgtg
    17761 cgcgcgatcc agcgctcgat gcaagccgcc aagcagcagt tcgccggcat gacgcgcgag
    17821 gagatcgcgc tgtataccgt cctcacgaac gcgccgctgc tgctgaccac ggtgctcggg
    17881 ctcgcgagtc gtttccctgc tttcagcacg gtggtctcga acgtgcccgg gccgcgccag
    17941 acgatgtact ggaacggcgc gcgtctcgac ggcatctacc cggcctcgat tccgttccac
    18001 ggcttcgcga tgaacatcac gctggtcagc tacgccgaca agctggattt cggcatcgtg
    18061 gcgtgccggc gttcggtgcc cggtgtgcag cgcatcatcg atcacatgga ggaggcgctg
    18121 gtggagctcg aggaagccgc cggattgcgg cgcgcacgcc gtggcgtgcg ccacgcgcga
    18181 cgcgccggct gagacccgcg gatgcgccgg gtgacgcgga aacgggccat gcccaggggc
    18241 tcgctcgcgg cggcgtcgtt gctggccgcc ttgctcgcgg gctgcaccga ccaggcgcca
    18301 ccgcccgcac cgggtgccga cgcgcagggc cacagcgccc cgaccgcggc gaccatcagc
    18361 gtgaaccgtg ccgccgcgaa ggatctcgcg ctcgatgaca cgcgcgattt cgaggacgcc
    18421 cgccgcggcc tgatcgccag cgtgccggag ctggtggtcg cgggctccgg tgcgacgccg
    18481 atctgggaca tgccgggtta tgcgtttatc gagggcgcgg cgcccgacag cgtgaacccg
    18541 agcctgtggc ggcaggcgaa gctcaacaac atccacgggc ttttccaggt cagcgaaggc
    18601 atttaccagt tgcgcggctt cgatctgtcg aacatgacgc tgatcgaggg cgagcaggga
    18661 tggatcgtgg tcgacccgct gacctcgcgc gagaccgccg cgcgtgcgct cgcctttgcg
    18721 cgcgagcacc tcggcgatcg gccggtcagc gcgatcatct acacgcacag ccacgtggat
    18781 cacttcggcg gcgtgctcgg cgtggcgagc gccgaggaga tcgccgcgcg cggcatccgc
    18841 gtgatcgcgc ccgaaggttt catggaggag gcgaccagcg agaacatgct ggccggcacc
    18901 acgatgggcc ggcgcgccgc ctacatgtac ggccagcacc tggcgcacgc gccgcgcggg
    18961 catgtcgaca ccgggctcgg caaggggccc gccttcggca gcgccggcat cgtggcgccc
    19021 accgagaccg tgggccgcac cggcgaggaa ctcgtgatcg acggcgtgcg cttcgtgttc
    19081 cagtacacgc cgggctcgga ggcccctgcg gagttcacgt tctacctgcc ggatctgaag
    19141 gccttctgcg gcgccgagat cgtctcgcac accctgcaca acctctacac gctgcgcggc
    19201 gcgaaggtgc gcgacgcgct gaagtggagc ggctatatcg acgaggcgat ccgtctgttc
    19261 ggcgatgcgg aggtgtattt cggcagccac cactggccgc tgtggggcga ggcgcgcatc
    19321 acggatttcc tgcagaagca gcgcgacacc tatcgctata tccacgacca gacgctgcgc
    19381 ctggccgcgc tcggacgcac gccgcagcag atcgccgacg agctggagct gcccacgtcg
    19441 ctcgcgcgcg agttcgccaa ccgcggctac tacggcaccg tgcggcacaa cgcgaaggcg
    19501 gtctaccagt tctacttcgg ctggtacgac gccaacccct cgcggctgaa cccgctggcg
    19561 ccggccgagg cggcgtcgcg ctacgtggag ttcatgggcg gcggcgccgc ggtgctggcg
    19621 aaagcgcagg cttctttcga caagggcgat taccgctggg ttgccgaggt gctgacccac
    19681 ctggtgttcg ccgagccgga caatggcgcg gcgcgcgaac tgctggcgcg cgcctacgac
    19741 cagctcggtt accaggcgga atcggggccg tggcgcgacg cctacctgag cggcgcgtgg
    19801 gagctgcgac atggcgtgcc gcaggaggcg ggcgatgtgg gcgcggtgct ggagctgctg
    19861 cgcaacacgc ccatagagcg cttcctcgac accatggccg tgcgcctcga cggcgcgaag
    19921 gcagccgagc ggcacaccgt catcaacctg gtgctgagcg ataccggcga cagccacgtg
    19981 ctcgaactgg ccaacggtgt gctgcaccac tggcgcgagc ccccacgcgc gcaagccgat
    20041 gccacgatca cggtcacgca cgcgctgttc atcgacctgc tgaccgggcg ggcgggacta
    20101 cgcgaaaccc tgggctccga tgcgctggaa gtcgacggca gccgtgtcga gctgctgcga
    20161 ttcttcgcgc tgttcgagca gcccgatacc gcgttcccga tcgtgacccc ggccgggtga
    20221 gtccgcacgc gaacgtgggt ccgcattcat gccgacgggt gccgcccggg tgcggcgggt
    20281 tcggcgttgc cgagcccttg tcgggctgaa gagggtgtcg caaaaccatc tgtgctctgg
    20341 atagcggtct gacaatccgg cgtatcgcga gccatcgcac cgatgggcgc tcgagcccct
    20401 cgccttggtg gaagccctag aaaagacgtg cattcccgga tttgcccgat gcgactcgcg
    20461 ctcgaccgag cggctgtgct gcccgctgtt ggcttgcggg gccttgcgcc gcaggacgcg
    20521 gcgcggagct acagggacgt actcgtgcgt gccccgcgag acaatggcgg gcagcacggc
    20581 cagcgacgca ccaagccgct cgctcgaaaa ataatggctt ttgcgacacc ctctgaagcc
    20641 cgacctactc cgacctacac caacct
//
LOCUS       DAORUQ010000034        19430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2421_length_19430_cov_6.264619, whole genome shotgun sequence.
ACCESSION   DAORUQ010000034 DAORUQ010000000
VERSION     DAORUQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19430
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_19430_cov_6.264619"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..3298)
                     /locus_tag="PLW13_04985"
     CDS             complement(89..3298)
                     /locus_tag="PLW13_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQY69757.1"
                     /translation="MKPAPASMHAASPVAADPLKLPGAGDNARRIFERAREMIYLRDG
                     AGRFTFVNRTGVRWLGLGNEQLLGRSEVELFDGRAGVNYRSWGNDAQTLRSGCARQFE
                     EMLTLRGRARHLMSIKLPIIDDSGGIGSLISIGIDVTRRKLMESALQNVALSITSVQG
                     ENVLRELVGYVCGSLQVDFALIGSVRDGVVDTLAVCDRGVAQEKFAYDLAGTPCEDVV
                     GKQFIIHPDNLLHHYPGNALLRRFGFRSYAGIPLFDSAGEAIGILAVLHRRPMRQPQL
                     VASLLKIYSVRAAAELERLRADAALREAEASYRAIFEAAEDPIFVHDWDSGAIVDVNG
                     KACEVYGCSREQLLCSTVGDFSAGDPLYTDARAMEWIERAKRDGAVEFAWHRRAADGS
                     LRWDEVRLKSALIGGKPRVLAFTRDITERKLSEEALRASEEQYRSIFNASTDGLLLFA
                     RDGRVIDVNPAFAALVGCSVAEVMHSPPHAFVAPESGENLRQLMATVVSGEFFEAEGM
                     ILHRDGHPIPVNVRGVPVVYRGDAHHLAIVRDISRHIEQQQQLVFSERRLRATVESSL
                     DCIMAIDRGGRIIEFNPMAEATFGIPAREAIGRNVALLIPERFHAVHEAAMARVLREG
                     GGGLIGRHLEVVVRRADGSEFDAELVVSTADSAEGRIVIGFLRDMTEHRVAQAERERL
                     EAQLRQAQKMEAIGHLAGGVAHDFNNLLTSLTGYVAMASERLALIGETRAGRYLQKSL
                     QSAERARHLIQQLLVFSRGQRGEPVAVQLTRHLGEFVDLLRSTLPSSLDFQLEFEPDV
                     PAVLVDPTQLDQVLMNLCINARDAMEGQGAMRVTVRRRQCRDGICASCQAAVAGDFVE
                     LAVADTGQGIAPAVLERVFDPFFTTKPQGKGTGMGLSTTHGIVHDGGGHILVESAVGK
                     GSTFRVLLPVLDAATGHVTPEAGAAVAPVAAPRLSGSVLVVDDDAHVLEFMEELLRDW
                     GLEVRALRDPQAALAGVASGEIRFDWAILDQTMPGMSGLELSRRLCDVLPDPRILLYT
                     GYSNQISEVAVAESGVRELLHKPLDHARLLRLLSDTPAR"
     gene            complement(3425..4153)
                     /locus_tag="PLW13_04990"
     CDS             complement(3425..4153)
                     /locus_tag="PLW13_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY69758.1"
                     /translation="MDQRHAVLVVDDDPSIREMLAEYLSERDCTVFAAADCGQAREFV
                     AARPCGVVLLDIGLPGEDGLSFARYLRERHDIGIIILSGAGETIDRIIGLEIGADDYL
                     AKPFDLREFHARLKSVARRYLRPVVSSVAAAQGAAPGTRQLSVGDCRLDLDRQQLFGA
                     SGEEIAVTAMEFDLLRVFAERPNRPLSRDTLLDLTQNREWDPFDRSIDIRIARLRRKI
                     EADPEKPRIIRTVRGVGYMFCRSD"
     gene            complement(4312..5103)
                     /locus_tag="PLW13_04995"
     CDS             complement(4312..5103)
                     /locus_tag="PLW13_04995"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQY69759.1"
                     /translation="MTPSNRASITNRIAVFCYGVICYAIFFATFLYLAGFIGNVLVPT
                     SIDGPPRVGMLRGLLTDLGLLGLFAVQHSVMARPAFKRWWTRFVPQAAERSTYVLFSS
                     IALIAMFAFWEPLGGLVWNLDGHAGRNLMWVAFAGGWLLVLIATFLINHFDLFGLRQV
                     WLNLLGRPYTPLRFATPWLYRRVRHPLYVGWLFAFWATPTMTLTHLVFALALTAYILV
                     AIRYEERDLIEVHGESYRRYREQVPMLVPRPGRAASGAGGQPLSA"
     gene            5386..5844
                     /locus_tag="PLW13_05000"
     CDS             5386..5844
                     /locus_tag="PLW13_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HQY69760.1"
                     /translation="MTEVRIPATHVGILETALLAMVSTIRHKDGLISTNPVGFDWDGE
                     YVRLSTLKSRVKYRNLVANPQITFCVVDPQMGTRYIEIRGHAQLSDDPEGSLNRKMFR
                     RMTGKEFDLDEPGAERVIIKIIPTQVSTPALYGGRLDRRAADVARSATPV"
     gene            5855..6586
                     /locus_tag="PLW13_05005"
     CDS             5855..6586
                     /locus_tag="PLW13_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY69761.1"
                     /translation="MFSSILEHYNWQAAAAVVLLTAAIIKTGKYLVFRIPAIRRMRDI
                     NIEEDNKRLAQSKYPAMISRGRKAGFACLFPFYFLILPFCATFAYVSLARVLLEIFVI
                     LMVYDFFYYLTHRFLLHAPHDSRLGYFRRVHAVHHQARDPSWVDATYVHPLEIAIGVL
                     LFLGTIVAHALVAGPAHVASLVVCFLAFHMINQVNHTYFKLPYFPFRTINWIVAKHHV
                     HHENMQKGNYATITLFYDRVFGTLD"
     gene            complement(6606..7556)
                     /locus_tag="PLW13_05010"
     CDS             complement(6606..7556)
                     /locus_tag="PLW13_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007105033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY69762.1"
                     /translation="MARLRPLGGSGLSIAPLVLGGNVFGSNGMDRARSFAVLDAFVAG
                     GGTMIDTADIYANYLPGGKGGESETMIGDWLAHRGRRDDVQIATKVGGPMTPQRKGLG
                     ATNIASAVEDSLRRLRCDYIDLYFAHFDDADVPQEEVAHAFERLVRAGKVRSVGASNF
                     APARLASALDIAAREGVARYTVMQPLYNLLERDFEAALRGLCLERDLGVIAYFGLARG
                     YLTGKYRSIDDLSKSRRGSGVRAYIEGKGPRVLAVMDAIAADRGTGLAAIALAWLMAK
                     PGVTAPIASATSVEQLAELMDALTLELTPGEMRRLDAAGA"
     gene            complement(7613..8146)
                     /locus_tag="PLW13_05015"
     CDS             complement(7613..8146)
                     /locus_tag="PLW13_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY69763.1"
                     /translation="MEAKLRELVARSEIWQVLQRYARGLDRIDNELARSCYWDDAIED
                     HNHFVGSPDGFIAFADRATLGFVSCQHGLLNHSCELAGDDAYCETYYIFTGEMATAPH
                     FMSTGRYIDHFQRRGGEWRIANRVTIVEGHYELGESQLGKGMPPAYGPGETRPASRDR
                     NDISYHRPPVPRRPKTG"
     gene            8198..11068
                     /locus_tag="PLW13_05020"
     CDS             8198..11068
                     /locus_tag="PLW13_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69764.1"
                     /translation="MTFARAARDTGSHCQGYSFFVAGVVMVEQTEAGAEVVLQILAEL
                     LGPGAAAHARMTPDARFDRDLGLDSLGRMELYRRVEDRFGVALPEQRLVELNSVRELW
                     QVVAAARTGALGTIGRADLPPPPGAGTPAQATTITDVLRWHAEHQGSRRYLTFFEDHG
                     DGPTLSFGELYARSAETAAGLQAAGVEPGDRVALMLPTCLEYFFAFFGTLLAGAVPVP
                     VYPPARRSELEDHMRRQGGILENAGAQVLITVPEGRALARLTRAMVPSLREVLCVGEL
                     AGKSAALQPVTRHAGDLALLQYTSGSTGAPKGVMLSNANLLSNIRTAAGVLGATGDDV
                     FVSWLPLYHDMGLIGACLATLYVGAQLVIMSPLRFLLRPQRWLWAIHRHRGTLSAAPN
                     FAYELCLSKIDDKSLEGLDLGSWRATLNGAEAISARSMEGFCERFARYGFRREAMYPV
                     YGLAENSVALAFPKPGSLPRVDVVQREALARRGLAVPAAADDPGALRFVCCGEAIPGH
                     EIRIVDAAGVEVGEREQGRIQFRGPSATAGYYRNPEATRRLIRGSWLETGDLGYMAGG
                     GLHITGRSKDIVIRNGRNIYPEEIEHAVGELPGVRRGRVAVFGSPEPASGRERLVVVA
                     EAHPAHITTPEQLIPAINRVCSEVAGSPPDVAMLVPGGTILKTSSGKLRREACRALYE
                     QGALGRAPPRPWRQFASLLLRALPGALSRLRRSLGVWAYNGYAWTVIGLLRAALWAGV
                     MAWPGQDGRWRMAHGAARAALRLVGAAPRIVNAERLHGLRRPLVVVANHSSYLDVVLL
                     AALLPWPASFVAMRELGQHYWTQRFYARIGTLFVEREDFTASVRDAARITAALRAGTS
                     LVVFPEGRLASIADLLPFRSGAFVAAVETGAAILPVTLVGAHAFMKGNRRLLRRGPLA
                     IHVGEPIVPDASPDDRWLEAQALGARARETIARTLAAADESR"
     gene            complement(11082..12653)
                     /locus_tag="PLW13_05025"
     CDS             complement(11082..12653)
                     /locus_tag="PLW13_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69765.1"
                     /translation="MDIKRCGLRAPTIRGRAWWRAVAGWALPGVLGLAATTAMASIPA
                     TPVMTLYRFNGDIELPYFERESFERRGARSPAGTLAQGTTLIPCLPVRHGRALTDDKG
                     TPFVGFEIVVDSRNATPEAAARLVEAIAARKALKVRNHHCGRGVRHVIDVRNLYAMEK
                     RPFFDPPGAGTGRPETAEGPDAIVRAFHNSALCAQANGRLMQRRQALKAAWDGFSREN
                     AGRWPAGDMARARHLDFTMRTALFEGHLERGCNAYGACERNIVALSIRNRALERCFAR
                     EGCRASGDFEGVASSVSQYNIWDEYLTQISGLAACFLRDDLATEDHYRRLQAMYAQNV
                     GDVQRILFGTDADLKELFPGAALADIKRTAHYYHAPAMGKCFPRHPRVEYMAGAVARH
                     GGDFALIANTRIEVGDKVGKDYRFRSVRVEETEARDIVDVRDDYPGFLVDGRKVTLAP
                     PTGCRPYGIPAGCPLGEVGRYRKTPSWLDAGKPLGLNCTIADRAAQCDAPVRLVNVQV
                     GGACDTQMRPVSGVH"
     gene            complement(12666..13547)
                     /locus_tag="PLW13_05030"
     CDS             complement(12666..13547)
                     /locus_tag="PLW13_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69766.1"
                     /translation="MSAMAATDEFQPVSRVFTSQGLDLHYLDWGNDGAPLLLLLHGSR
                     DHARSWDWTARALRHQWHVIAPDLRGHGDSDWSSDGAYLTVFHALDIAVLIETLGERQ
                     LCIVGHSFGGGVAIRYAATFPERVRKLVLVDGLGPSPQVQASWDRQGPVRRTREWIDN
                     RLAVGARAQRRFATLEEAVARMAEANRHLSEEQARHLAVHGVRRHADGYGWKYDPRVF
                     IFAPDDFALDSSAFWRAIAAPVLLCHGTESWTTNAEEDGRAGHFRDRRTVVFENAGHW
                     IHHDRCDAFIAALRDFL"
     gene            13715..>15189
                     /locus_tag="PLW13_05035"
     CDS             13715..>15189
                     /locus_tag="PLW13_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69767.1"
                     /translation="MPAEDLHRPLYTPDLLVNALSSDPGRPLLHLLDGPTLTVGEVRD
                     ATSQFVQALASLGVKRGTRVGLIASNRPEVLHVSHAVQLLAAIYVPMHPLGGLADHRH
                     VVEDSGVEILVFDAGRYAGRAAELAQAVPALRLLAIGDSPLGADICALAGSFAPSPLV
                     APGVGPHDVMRLGYSGGTTGKPKALASVHRTGVTTLQIMLSDWEWPNPPRVLSCTPLS
                     HAGSAMFLPTLMKGGTMLVLPGFDPVAVLQAIQDHRINCVLLVPTMIYALLDHPRFEE
                     FDLSSLETVFYGASAIAPSRLREAIERIGPVFCQFYGQAEAPMAITMLRKSEHDVNDM
                     RRLASCGRPSPWVRVELLDSDNRPVPDGEPGEICVRGPLVMDGYRDNPQLTAQAFAGG
                     WLHTGDVAVRDPGGFLRIIDRTKDMIVTGGFNVYPREIEDIIAQHPAVSQVAVIGVPH
                     PRWGEAVKALVVLRPGQSATEEELIAPVAEAKGSFQAPKSV"
     assembly_gap    15190..15289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15464..17437)
                     /locus_tag="PLW13_05040"
     CDS             complement(15464..17437)
                     /locus_tag="PLW13_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HQY69768.1"
                     /translation="MPFSVFRGARRAALLLAVFSPSLLAGPATGAANEWPVWGGDAGA
                     THFSGLTDITPANVASLEVAWTHRSGDHADGSGRTRATYFQATPLAVNGRLYYCTPFQ
                     RVFALDPETGAELWQFDPAVKTLTGEGPYPLNCRGVTYWEDSAAPAGAFCARRIFYGT
                     SDSELLALDADTGRPCGDFGTDGRVDLRRDLDEPRAWAVHPTSPPLVMRDRVVINGFV
                     ADNLEVDAAPGMVRAFDARTGALAWAWNPVPPGWQPRPSARKSPWQAGAPNSWSIITG
                     DHERGLVFVPTGNPSPDLYGGERHGIDHYGSSTVALSIETGQVVWSFQGVHHDVWDYD
                     TPSAPILFQADGVGGGVPAVAQTTKVGHIFLLDRVSGAPLYPVEERPVPQDGVPGETL
                     SPTQPFPTHPAPMHPSAMAPEDAHGFTPLDRRDCARKIARYRWDGAFTPPTLAGSIGY
                     PHSSGGMNWGSAAIDSGRGLLIVNQNHIAQVVQLIPREEAAGLDPAAQQYPNEVYPMN
                     GTPYVARRGPLESFLGAPCSPTPWGSLAAVDLRSGEVKWRIPFGTLEGLAPWPVPALF
                     PDTGAPNFGGGIATASGLFFIGASMDGYFRAYSSADGSELWKTRLPYSGHAVPMTYRG
                     TSGAQFVVIAAGGNALSRMGAELIAFRLPAAAP"
     gene            complement(17529..18635)
                     /gene="glf"
                     /locus_tag="PLW13_05045"
     CDS             complement(17529..18635)
                     /gene="glf"
                     /locus_tag="PLW13_05045"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HQY69769.1"
                     /translation="MPRRYHFLIVGCGFTGATLAERLAAHGARVLVIDRRDHIGGNAY
                     DRLDEHGIRVGPYGPHLFHTRSEKIWRYVNRFATFNDYELRVQALHGGRYYDLPLNLS
                     TFRAFFGNDLREQDVPALLAELREPIEQPRNAEEAIVSQVGRALYRAFYEGYTRKQWG
                     MEPRELDASVTLRLPIRHHEDNRYFSDRWQGLPIEGYTRLFERMLAHENITVALNADD
                     RAVRASERFDKLIYTGPIDAYFDHALGRLPYRSIDFRLESIASRELVQRVGLLNYPDQ
                     RAYTRSAEYRHFYGQSSDWTTLGRDYPCWNDAEPYYPVPNPANRALYARYKALAAAET
                     DTLFCGRLGTYKYYNMDQCIAQALTLAERLLRGN"
     gene            complement(18635..>19430)
                     /locus_tag="PLW13_05050"
     CDS             complement(18635..>19430)
                     /locus_tag="PLW13_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69770.1"
                     /translation="GAQLNPVIAVLVKTHFIDDALLAQLRTMRRALLGQPRVELVLGV
                     DAAAVPAVALAAVEAALPGLRLHGFHRDRYADHGLRPHAFLAGMGAPLDWYHSDYSLI
                     DFHLHHPQRYTALWQIEYDVSLRSGSWDFLGRDLPFDLLVPGLKIRERGLRALVHSAC
                     ILQPDWFWWDKLDGHAATVGGFFPCIRITPRALDHLVSVYRAGVSGYCEVSVPSILAC
                     APGMRVAALSVIDTAEVELTHPHWDKERRGDRQQDLLLNRRGLYVD"
BASE COUNT         3114 a         6701 c         6461 g         3054 t
ORIGIN      
        1 gtacgcgcga ctcgtttcgg tccaattgcg caatgcagcc gcgttgttgc cggattgttt
       61 cgcgactgct gcccgcgcgg cacgagcatc aacgcgccgg cgtgtcactc agcaggcgca
      121 acagccgagc atggtccagg ggcttgtgca gcagttcgcg cacgccagac tcggccacgg
      181 cgacctcgct gatctggttg ctgtaaccgg tatagagcag gatgcgcggg tcgggcagca
      241 cgtcgcacag gcgccgtgac agctcgagcc cgctcatgcc gggcatggtc tggtccagta
      301 tcgcccagtc gaagcggatc tcgcccgacg caacgccggc aagcgccgcc tgcggatcgc
      361 gcagggccct cacctccagc ccccagtcgc gcagcagttc ctccatgaac tcgagtacgt
      421 gcgcatcgtc atcgaccacc agcacggatc cgctcagccg cggtgccgcg accggcgcta
      481 cggccgcgcc ggcttcgggc gtcacgtgcc cggtcgcggc atcgagcacc ggcagcagga
      541 cgcggaaggt cgaacccttc cctaccgcgc tctcgacgag tatgtgtccg ccgccgtcgt
      601 gcacgatgcc atgagtggtg gacagcccca tgccggtgcc cttgccctgg ggcttggtgg
      661 tgaagaacgg gtcgaacacg cgctcgagca cggccggcgc gatgccttgc ccggtgtcgg
      721 caacggcgag ttcgacgaaa tcgcccgcta ccgcggcctg gcacgaggcg cagataccgt
      781 cacggcactg gcgccggcgc acggtcacgc gcatcgcgcc ctgcccctcc atcgcatcgc
      841 gcgcgttgat gcagaggttc atcagcacct ggtcgagttg cgtcgggtcg accagcaccg
      901 ccggcacatc cggctcgaac tcgagctgga aatccaggct cgatggcagc gtcgagcgca
      961 gcaggtcgac aaattccccg aggtgacgcg tcaattgcac cgccaccggt tcgccgcgct
     1021 ggccgcgact gaacaccagc agctgctgga tgaggtggcg cgcccgctcc gccgactgca
     1081 gcgacttctg caggtagcgg cccgccctgg tttcgccgat cagcgccagg cgctcgctgg
     1141 ccatggccac gtagcccgtg aggctggtca gcaggttgtt gaaatcgtgc gccacgccgc
     1201 ccgcgaggtg gccgatggcc tccattttct gggcctggcg caactgcgcc tcgaggcgtt
     1261 cgcgctcggc ctgcgcgacc ctgtgctcgg tcatgtcgcg caggaagccg atgacgatgc
     1321 ggccctcggc gctgtcggca gtggaaacca ccagctcggc atcgaactcc gagccgtccg
     1381 cgcgccgcac cacgacctcg agatgtcgac cgatcaggcc gccgccgccc tcgcgcaaca
     1441 ccctcgccat cgcggcctca tgcaccgcat ggaagcgttc cgggatcagc agcgcgacat
     1501 tgcggccaat ggcctcgcgt gccgggatgc cgaaggtggc ttcggccatc ggattgaact
     1561 cgatgatcct tccgccacga tcgatcgcca tgatgcagtc cagcgaactc tcgacggtgg
     1621 cgcgcaggcg gcgctcgctg aataccagtt gctgctgctg ttcgatgtgg cgcgagatgt
     1681 cgcgcactat cgccaggtgg tgcgcatcgc cccggtagac caccggcaca ccacgcacgt
     1741 tgaccggaat cgggtgtccg tcgcggtgca ggatcatgcc ctcggcctcg aagaactcgc
     1801 ccgagaccac cgtcgccatc agctggcgca ggttctcccc gctctccggc gcgacgaagg
     1861 catgtggcgg cgaatgcatc acctctgcca ccgaacagcc gaccagggcc gcgaaggccg
     1921 gattgacatc gatgacgcgc ccgtcccggg cgaacagcag caggccgtcg gtggacgcat
     1981 tgaagatcga gcgatactgc tcctcgctcg ctcgcagcgc ctcctcggac agcttgcgct
     2041 cggtgatgtc gcgcgtgaat gccagcaccc gcggcttgcc gcctatcagg gcgctcttca
     2101 ggcgcacctc gtcccagcgc aggctgccgt cggcagcgcg gcgatgccac gcgaattcga
     2161 ccgcgccgtc gcgcttggcg cgctcgatcc actccatcgc gcgtgcgtcc gtgtaaagag
     2221 gatcgccggc gctgaaatcc cctaccgtcg agcacagtag ctgctcgcgg gagcaaccgt
     2281 agacttcgca ggccttgccg ttgacgtcga cgatcgcgcc gctgtcccag tcatggacga
     2341 atatggggtc ctcggccgcc tcgaagatgg cacggtagct ggcctcggcc tcgcgcagtg
     2401 ccgcgtcggc gcgcaaccgt tcgagttccg ccgcggcacg aaccgagtag atcttcagca
     2461 gggacgccac gagttgcggc tggcgcatgg gtcgccggtg aagcacggca agaatcccga
     2521 tcgcctcgcc ggcggaatcg aagagcggaa tccccgcgta gctgcggaaa ccgaatcgac
     2581 gcagcagcgc gttgccggga taatggtgca acaggttgtc ggggtggatg atgaactgct
     2641 tgcccacaac gtcctcgcag ggcgtgcccg cgagatcata ggcgaatttt tcctgcgcca
     2701 cgccgcgatc gcagacggcg agcgtatcga ccacgccatc cctgacgctg ccgatcagcg
     2761 cgaaatcgac ctgcaggcta ccgcacacgt agccgacgag ttcacgcagc acgttctcgc
     2821 cctgcacgct ggtaatgctg agcgcgacgt tctgcagcgc ggactccatc agcttgcgcc
     2881 gggtgacatc gatgccgatg ctgatcagcg atccgatacc gcccgagtca tcgattatgg
     2941 gcaacttgat cgacatcagg tgccgagcgc gaccgcgcag cgtgagcatt tcctcgaact
     3001 gccgcgcgca accgctgcgc agcgtctgcg cgtcgttgcc ccagctgcgg tagttcacgc
     3061 cggcgcggcc atcgaacagc tcgacctcgg aacggcccag cagttgttcg ttaccgagcc
     3121 ccagccagcg cacgccggtc ctgttgacga aggtgaaacg gcccgcgccg tcgcgcagat
     3181 agatcatctc gcgcgcccgc tcgaagatgc gccgcgcgtt gtcaccggcg cccggcagct
     3241 tcagcgggtc cgccgcaacg gggctggctg catgcatgct ggctggcgcg ggtttcatga
     3301 cacgtctcgc ccccggtctt gctgcgatcg accccagtct agcaaccgtg ccgtggcgcc
     3361 gttgcgcacg gaaacaaatg aatcaattga tacagtgccg atacacggcg cacccccgcc
     3421 cgcttcagtc ggagcggcag aacatgtagc cgacaccgcg cacggtgcgg atgatgcgcg
     3481 gcttctcggg atcggcttcg atcttgcggc gtagccgcgc gatgcgtatg tcgatcgagc
     3541 ggtcgaaggg atcccactcc cggttctggg tgaggtcgag cagggtgtcc cgcgacagcg
     3601 gacggttcgg gcgctccgcg aatacgcgca gcaggtcgaa ctccatcgcc gtgacggcga
     3661 tttcctcgcc cgaggcaccg aacagctgct gccggtcgag atcgagccgg caatcgccca
     3721 cgctcagctg ccgcgtgcct ggcgcagcgc cttgcgccgc ggcgacgctg gatacgacag
     3781 gccgaaggta gcggcgcgcc acgctcttca ggcgggcgtg gaactccctc aggtcgaagg
     3841 gcttggcgag ataatcgtcg gccccgattt ccaggccgat gatccggtcg atggtctccc
     3901 cggcgccgga gaggatgatg atgccgatgt cgtggcgctc gcgcaggtag cgggcgaaac
     3961 tcagtccgtc ttcgccgggc agcccgatgt ccagcaggac cacgccacag ggccgggccg
     4021 ccacgaactc ccgcgcctgg ccgcagtcgg ccgctgcgaa taccgtgcaa tcgcgttccg
     4081 agagatattc cgcgagcatc tcgcggatcg acgggtcgtc atcgacgacg agcaccgcat
     4141 ggcgctggtc catgctcacc tcggctgatt ctcgaccggg gagtcaactg gaggtttttt
     4201 actcctcggg gccgcaatgc acaagtcctc gccagccccg tgactgcggc acggcgaaag
     4261 ccagccgcaa acgcggttcg acccgcgccc ggctggcggt tgcgcgcccg gtcatgcgct
     4321 cagcggttgc cccccggcgc cgcttgctgc cctgccgggg cgcgggacca gcatcggcac
     4381 ctgctcgcgg tagcggcgat aggactcgcc gtgcacctcg atcaggtcgc gctcctcgta
     4441 gcggatcgcc acgagaatgt acgcagtcag cgccagcgcg aacaccaggt gcgtcagcgt
     4501 catcgtcggc gtggcccaga aggcgaacag ccagcccacg tagagcggat ggcgcaccct
     4561 gcggtagagc cacggcgtgg cgaaacgcag cggggtgtag ggcctgccga gtaggttcag
     4621 ccacacctgg cgcaggccga agaggtcgaa gtggttgatc aggaaggtgg cgatcagcac
     4681 cagcagccag ccgccggcga atgccaccca catgaggttc cggccggcat ggccgtccag
     4741 attccacacc aggccgccga gcggttccca gaacgcgaac atcgcaatca gcgcgatgct
     4801 cgagaacagc acgtaggtgc tgcgctctgc ggcctgcggc acgaaacggg tccaccagcg
     4861 cttgaaggcc ggccgggcca tgacgctgtg ctgcacggca aacagcccca gcagcccgag
     4921 gtccgtgagg agcccccgca gcattcccac ccgcggcggg ccgtctatcg atgtcggcac
     4981 gagcacgttg ccgatgaatc ccgcgagata gagaaacgtg gcgaagaaga tcgcgtagca
     5041 gatcacgccg tagcagaaca ccgcgatccg gttggtgatg gaagctctgt tgctgggtgt
     5101 catggcatcc tcccgtcggt tgctgtgccg gttgtgacgc tgcagacagt gcgggaaatc
     5161 cgtatcaggc ggatttccgc ctgctgcggg tttgttgtgc cattgtttcg gcgccgtaac
     5221 gcggcggcgc cgccatatcg ttcgcatggg gccgcggcat gacaccggtt taatgcgtca
     5281 tttgaacatc attgttgacc attcgacggt cgggcggcat gctagccgcc gatccggtcc
     5341 gcgccatccg cgggctcggg aaacgccagc attaatggag cattcatgac cgaggtccgc
     5401 attccagcca cccatgtcgg catcctggag accgccctgc tcgccatggt cagcactatc
     5461 cgccacaagg acgggctgat ttccaccaac ccggtcggct tcgactggga tggcgaatac
     5521 gtgcgcctca gcacgctcaa gtcgcgcgtg aagtaccgca acctcgtggc gaatccgcag
     5581 atcacgtttt gcgtggtcga tccgcagatg gggacccgct acatcgagat ccgcggccat
     5641 gcgcagctga gcgacgatcc cgaaggctcg ctgaaccgca agatgttccg ccgcatgacc
     5701 ggcaaggaat tcgacctcga cgagccgggc gccgaacgcg tgatcatcaa gatcatcccg
     5761 acgcaggtat cgacccccgc gctgtatggt ggccggctcg accggcgcgc ggcagacgtg
     5821 gcacgctccg caacccccgt ctgaggctgg cgtcgtgttt tcgtccatcc tcgagcatta
     5881 caactggcaa gcagccgccg cggtggtgct gttgaccgcc gcgatcataa agacgggcaa
     5941 gtacctcgta ttcaggatcc cggcgatcag gcgcatgcgg gacatcaata tcgaggagga
     6001 caacaagcgc ctcgcccagt caaagtaccc ggcgatgatc agcaggggca gaaaggccgg
     6061 ctttgcgtgc ctgttcccgt tctatttcct gatcctgccg ttttgcgcga ctttcgcgta
     6121 cgtctccctt gccagggtgc tgctcgagat cttcgtcatc ctgatggtct atgacttctt
     6181 ctactacctg acccatcgct tcctgctgca tgcgccgcac gacagccgcc tgggatactt
     6241 ccgccgcgtg cacgcggttc accaccaggc gcgcgatccg agctgggtcg atgcgaccta
     6301 tgtgcatccg ctcgaaatcg ccatcggcgt gctgctcttc ctcggcacca tcgtcgccca
     6361 cgcgctcgtc gccgggccgg cgcacgtggc gtcgctcgtc gtgtgcttcc tcgcgttcca
     6421 catgatcaac caggtcaatc acacgtattt caagctgccg tacttcccgt tcaggacgat
     6481 caactggatc gtcgcgaagc accatgttca ccacgagaac atgcagaagg gaaactacgc
     6541 cacgatcacg ctgttctacg acagggtctt cggtacgctg gactgattcg gcgctgcgcg
     6601 gcgtatcagg cgcccgccgc gtcgagccgg cgcatctccc cgggcgtaag ttccagtgtc
     6661 agggcatcca tgagctcggc gagttgctcc acgctggtcg cgctcgcgat gggtgccgtg
     6721 acgccgggtt ttgccatcag ccacgccagc gcgatcgccg ccagcccggt gccgcgatcg
     6781 gcagcgatcg cgtccatgac ggcgagaacc cgcggcccct tcccttcgat gtacgccctg
     6841 acgccgctgc cacgccggct cttcgacaga tcgtcgatcg agcggtactt gcccgtcaga
     6901 tagccccgcg ccagaccgaa gtacgcgatc acaccgagat cgcgctccag gcagagtccc
     6961 cgcagggccg cctcgaagtc gcgctcgagc aggttgtaga gcggctgcat cacggtgtat
     7021 cgcgcgactc cctcgcgcgc ggcaatgtcg agggcgctcg cgagccgggc gggcgcgaag
     7081 ttcgaagcgc ccacgctgcg caccttgccc gccctgacca gccgctcgaa cgcgtgcgcg
     7141 acctcctcct gcggcacatc ggcgtcgtcg aagtgcgcga aatagaggtc gatgtaatcg
     7201 cagcgcagcc tgcgcaggga atcctcgacc gcgcttgcga tgttggtcgc gccgagaccc
     7261 ttgcgctgcg gcgtcatcgg tccgccgacc ttggtcgcga tctgcacgtc gtcgcgtcgg
     7321 ccccggtgcg cgagccagtc gccgatcatc gtctccgatt caccgccctt tccgcccggc
     7381 agatagttcg cgtagatgtc cgccgtgtcg atcatcgtgc ctccgccggc aacgaacgca
     7441 tcgagcacgg cgaaactgcg tgcgcgatcc atgccgttgc tgccgaagac gttgccaccg
     7501 agcaccagcg gcgcgatcga aagcccgcta ccgccgagcg gacgaagcct ggccatgcat
     7561 accctccccg atacgtcgtc gaactgcgcg cagcgccacg gtgcgcggga tctcagcccg
     7621 tcttcggccg ccgcggcacc ggcgggcggt gatagctgat gtcgttgcga tcgcggctcg
     7681 caggcctggt ttccccgggg ccgtaagcgg gcggcatgcc cttgccgagc tgcgattcac
     7741 cgagctcgta atggccctcc acgatggtca cgcgattggc gatgcgccat tcgccgccgc
     7801 ggcgctggaa atggtcgatg tagcgcccgg tcgacatgaa atgcggcgcc gtggccatct
     7861 cgccggtgaa gatgtaatac gtttcgcaat acgcatcgtc gccggccagc tcgcagctgt
     7921 ggttcagcag gccgtgctgg cagctgacga aacccagcgt cgcccggtcg gcaaaggcga
     7981 tgaatccatc cggcgagccg acgaaatggt tgtggtcctc gatcgcatcg tcccagtagc
     8041 aggaacgcgc cagctcgttg tcgatccggt ccagcccgcg cgcatagcgt tgcagcacct
     8101 gccagatctc gctgcgcgca accagttccc tgagcttcgc ctccatcgca cctacctttt
     8161 cctgatgaat tgcacagcct cgcatgcctg aggcaccatg acattcgcgc gtgccgcgcg
     8221 cgataccgga tcgcattgcc aaggttactc cttcttcgtt gcaggtgtcg tgatggtcga
     8281 gcagacagag gccggtgcgg aggtggtgct gcagatcctg gcagagctgc tcggaccggg
     8341 agccgcggcc cacgcgcgaa tgacgccgga tgcccgcttc gaccgcgacc tcggactcga
     8401 cagcctcggg cgcatggagc tctaccgccg cgtcgaggac cgcttcgggg tggcgctgcc
     8461 ggagcaacga ctcgttgaac tgaacagcgt gcgcgaactg tggcaggtcg tcgcggccgc
     8521 tcgcaccggc gccctgggca cgatcggccg cgccgacctc ccgcccccgc cgggcgcggg
     8581 aacccccgcg caggccacga caatcacgga tgtcctgcgc tggcacgccg agcatcaggg
     8641 ctcgcgccgc tacctgacgt tcttcgagga ccatggcgac gggcccacgc tcagcttcgg
     8701 ggaactgtac gcgcgctctg ccgagacggc tgcgggcctg caggccgcgg gcgtggagcc
     8761 gggcgatcgc gtggccctga tgctgcccac ctgcctcgag tacttcttcg cgttcttcgg
     8821 cacgctgctc gccggcgccg tacccgtgcc cgtttacccg ccggcgcggc gcagcgagct
     8881 cgaggaccat atgcgccgcc aaggcggcat cctcgaaaac gccggagcgc aggtgctcat
     8941 caccgtgccc gagggtcgcg cgctcgcccg cctcacccgc gccatggtgc cctcgctgcg
     9001 cgaggtgctg tgtgtcgggg aactggccgg caaatccgcc gcgctgcaac ccgtgacgcg
     9061 gcacgcgggg gatctcgcgc tgctgcagta cacctccggc agcaccggcg cgccgaaggg
     9121 cgtgatgctg agcaatgcaa acctgctgag caatatccgc acggccgcgg gtgtactggg
     9181 cgcaaccggc gacgacgtct tcgtgagctg gctgccgctc taccacgaca tgggcctgat
     9241 cggcgcctgc ctcgccacgc tctacgtcgg cgcgcagctg gtcatcatgt cgccgttgcg
     9301 tttcctgctg cgcccgcagc gctggctctg ggccatccac cgccaccgcg gcacgctgtc
     9361 ggccgcgccg aacttcgcct acgagctgtg cctgtcgaaa atcgacgaca agagcctcga
     9421 gggactcgac ctcggcagct ggcgcgcgac gctcaacggc gcggaggcga tcagcgcgcg
     9481 ctccatggag ggcttctgcg agcgctttgc gcgctacggc ttcaggcgcg aggccatgta
     9541 cccggtctac ggcctcgcgg agaacagcgt cgcgctggcg tttccgaaac ccggctcgct
     9601 gccgcgcgtg gacgtggtac agcgcgaggc gctcgcgcgc cgcggcctgg ccgtgccggc
     9661 ggcggcggac gaccccggcg cgctgcgctt cgtgtgctgc ggcgaggcga ttcccgggca
     9721 cgagatccgc atcgtcgatg ccgccggcgt ggaagtgggc gagcgcgagc aggggcgcat
     9781 ccagttccgc ggcccctcgg caaccgccgg ctattaccgc aatccggagg ccacgcgccg
     9841 gctgatccgg ggcagctggc tcgagacggg cgatctcggc tacatggcgg gcggcggcct
     9901 gcacatcacc ggccgctcga aggacatcgt gatccgcaat ggccgcaaca tctaccccga
     9961 ggagatcgag cacgccgtgg gtgaactccc cggcgtgcgc cgcggccgcg tcgccgtctt
    10021 cggcagcccg gaacccgcga gcgggcgcga gcgcctggtc gtggtcgccg aggcgcatcc
    10081 ggcgcacatc accacgcccg aacagctgat cccggcgatc aaccgggtct gcagcgaggt
    10141 cgcgggatct ccgcccgacg tcgcgatgct ggtgccgggc ggaaccatcc tcaagacctc
    10201 gagcgggaag ctgcgccgcg aggcgtgccg ggcactgtac gaacagggag cgctcgggcg
    10261 cgcgccgcca cggccgtggc gccagttcgc aagcctgctg ctgcgggcgc tgccgggcgc
    10321 gctatcgcgg ctgcgtcgca gtctcggcgt ctgggcctac aacggctatg cctggaccgt
    10381 catcggcctg ctgcgcgccg cgctatgggc tggcgtgatg gcatggccgg ggcaggacgg
    10441 acgctggcgc atggcgcatg gcgcggcacg cgcggcgctg aggctcgtcg gggcggcgcc
    10501 gcggatcgtg aacgccgagc gcctgcacgg cctgcgccga ccgctggtcg tggtcgccaa
    10561 tcacagcagc tatctcgacg tcgtgctgct ggcggcgctg ctgccctggc ccgccagctt
    10621 cgtggcgatg cgcgaactgg gccagcacta ctggacgcag cgtttctacg cgcgcatcgg
    10681 cacgctgttc gtggagcgcg aggacttcac cgcgagcgtg cgcgacgcgg cccgcatcac
    10741 ggctgcgctg cgagccggta ccagcctggt cgtgttcccc gaggggcgtc tcgcatcgat
    10801 cgccgatctg ctgccctttc gcagcggcgc cttcgtcgcg gcggtcgaga ccggcgcggc
    10861 catcctgccc gtcacgctcg tgggcgcgca tgcgttcatg aagggcaatc gccgcctgct
    10921 gcggcgcgga ccgcttgcca tccacgtggg ggaaccgatc gtgccggacg cgagccctga
    10981 cgatcgctgg ctcgaggcgc aggcgctcgg cgcgcgcgcc cgcgagacca tcgcgcgcac
    11041 gcttgccgcc gcggacgaat cgcgctgagc cgacgcctgc gtcaatgcac acccgagacc
    11101 gggcgcatct gcgtatcgca ggcaccgccg acctgcacgt tcaccagccg caccggggcg
    11161 tcgcactgcg cggcgcgatc ggcgatcgtg cagttcaggc cgagcggctt gcccgcgtcg
    11221 agccacgacg gcgtcttgcg gtagcggccg acctccccca gcgggcagcc ggccggtatg
    11281 ccgtagggtc ggcagcccgt gggcggggcg agggtgacct tgcgcccgtc gaccaggaag
    11341 ccgggataat cgtcacggac atccacgatg tcgcgcgcct cggtctcctc gacgcgaacc
    11401 gaacggaaac gatagtcctt gcccaccttg tccccgacct cgatccgcgt gttggcaatc
    11461 agcgcgaagt cgccgccgtg tcgcgccacc gcgccggcca tgtattccac gcgcgggtgg
    11521 cgcgggaagc atttgcccat cgccggtgcg tgatagtagt gggccgtgcg cttgatgtcg
    11581 gcgagcgccg cgcccggaaa gagttccttc aggtcggcat cggtgccgaa gaggatccgc
    11641 tgcacgtcgc cgacgttctg cgcatacatg gcctgcagcc ggcggtagtg atcctccgtc
    11701 gccagatcgt cgcgcaggaa gcaggccgcg agccccgaga tctgggtgag gtactcgtcc
    11761 cagatgttgt actgcgagac gctcgaggca accccctcga agtcgccgga ggcccgacag
    11821 ccttcgcgcg cgaaacagcg ctccagggca cggttgcgaa tcgacaacgc gacgatgttg
    11881 cgctcgcagg cgccgtaggc gttgcagccg cgctcgagat gcccctcgaa cagcgcggtg
    11941 cgcatcgtga aatcgaggtg gcgcgcccgg gccatgtcgc ctgccggcca gcggccggca
    12001 ttctcgcggc tgaaaccgtc ccacgccgcc ttgagcgcct ggcgccgctg catgagcctg
    12061 ccgttcgcct gcgcgcacag ggcactgttg tggaacgcgc gcacgatggc atcgggacct
    12121 tcggcagtct cgggacggcc cgtgcccgcg ccgggcggat cgaaaaacgg gcgcttctcc
    12181 atcgcgtaga gattgcgcac atcgatgacg tggcgcaccc cccgcccgca atggtgattc
    12241 ctgaccttca gcgccttgcg cgcggcgatg gcctccacta gcctggccgc ggcctccggc
    12301 gtggcgttgc gtgaatcgac gacgatctcg aaaccgacga agggcgtgcc cttgtcatcg
    12361 gtcaacgcgc gaccgtgccg cacgggcagg cagggaatca gcgtggtgcc ctgggccagc
    12421 gtccccgccg gcgatcgcgc cccccggcgc tcgaaactct cgcgctcgaa atacggcagc
    12481 tcgatatcgc cgttgaagcg gtagagcgtc atcaccggcg tcgcggggat gctcgccatc
    12541 gcggtcgtcg ccgcgagacc gagcacgccg ggcaacgccc accctgcgac ggcgcgccac
    12601 cacgcccggc cacggattgt cggggcgcgg agcccgcagc gcttgatgtc catcgtgccc
    12661 tcggcttaca gaaagtcgcg caaggccgcg atgaaggcat cgcagcgatc gtggtggatc
    12721 cagtgccccg cgttctcgaa aacgacggtc cgccggtcgc gaaagtgccc cgcgcgccca
    12781 tcctcttccg cgttggttgt ccagctctcg gtgccgtggc agagcaggac cggcgcagcg
    12841 atggcgcgcc agaacgcgct gctgtcgagc gcgaagtcgt cgggggcaaa aatgaatacc
    12901 cgcggatcgt atttccagcc gtagccgtcg gcatgccggc gcaccccgtg aacggcgagg
    12961 tgacgcgcct gctcctcgct caggtgccgg tttgcctcgg ccatgcgcgc aacggcttcc
    13021 tcgagcgtcg caaagcgccg ttgcgcccgc gcgcccacgg cgagccggtt gtcgatccat
    13081 tcgcgcgtgc gccggacggg cccctgccgg tcccagctgg cctgcacctg cggcgacggg
    13141 ccgagcccgt ccaccagcac cagcttgcgc acccgctcgg gaaaggtcgc cgcgtagcgg
    13201 atcgccacgc cgccgccaaa ggaatggccc acgatgcaca gctgccgttc cccgagcgtc
    13261 tcgatcagca ccgcgatatc cagcgcgtga aagaccgtga gataggcgcc gtcgctcgac
    13321 cagtcactgt cgccatggcc gcgcagatcg ggtgcgatca catgccactg atgccgcaat
    13381 gcgcgcgccg tccagtccca gctgcgcgcg tgatcgcggc tgccgtggag cagcaggagc
    13441 agcggcgcgc cgtcgttgcc ccagtcgagg taatgcagat ccagcccctg ggaggtgaaa
    13501 acgcgggaaa ccggctggaa ctcgtcggtg gcggccatgg cgctcatgca gtgtccttgg
    13561 gcatgccgcg tgtgccgtcg cgccggttcg aaccggatgc ggcagcctcg cgcttgcagg
    13621 tgcaacggaa gaccggcgct atcgtaaacg atccggcttg cgagcccgca ggaggacgcg
    13681 tactttcgcg actccggcac cccgaggagt tcccatgccc gccgaagacc tgcaccggcc
    13741 gctctatacg ccggacctgt tggtgaacgc gctcagcagc gatcccgggc gcccgctcct
    13801 gcacctgctc gacggcccga cgcttaccgt gggggaggtc cgtgacgcca ccagccagtt
    13861 cgtgcaggcg ctggccagcc tcggcgtgaa acgcggaacc cgcgtcggcc tcatcgcctc
    13921 caaccggccc gaagtgctgc atgtgagtca tgcggtgcaa ctgctggcgg cgatctacgt
    13981 gccgatgcac ccgctgggcg gccttgccga ccaccgacac gtcgtggagg attccggggt
    14041 cgagatcctg gtattcgatg ccggccgcta tgcgggccgt gccgcagaac tggcgcaggc
    14101 ggtgccggcg ctgcggctgc tcgcgatcgg cgacagcccg ctcggcgcgg acatatgcgc
    14161 cttggccggg agcttcgcac cctcgccgct ggtcgcaccc ggggtcgggc cgcacgacgt
    14221 catgcgcctg ggctactcgg gcggcaccac cggcaagccc aaggcactgg ccagcgtgca
    14281 ccgcaccggc gtgaccaccc tgcagatcat gctgtcggac tgggaatggc ccaacccgcc
    14341 gcgggtgctg tcctgcacgc cgctcagcca tgccggctcg gccatgttcc tgccgacgct
    14401 gatgaagggc ggaaccatgc tggtgctgcc gggcttcgat ccggtcgcgg tgctgcaggc
    14461 catccaggac catcgcatca actgcgtgct gctggtcccg acgatgatct acgccctgct
    14521 cgaccatcca cgcttcgagg agttcgacct ctcgagcctg gagacggtgt tctatggcgc
    14581 atcggctatc gcgccttccc gcctcaggga agcgatcgaa cgcatcggcc cggtcttctg
    14641 ccagttctat ggccaggccg aggcgccgat ggcaatcacg atgctgcgca aatccgagca
    14701 cgacgtgaac gatatgcgtc gcctggcaag ctgcgggcgt ccctcgccgt gggtgcgtgt
    14761 cgaactgctc gacagcgaca accggccggt gcccgacggg gaaccgggcg aaatctgcgt
    14821 gcgcggaccg ctggttatgg acggttatcg cgacaacccg caactgacgg cccaggcctt
    14881 cgctggcggg tggctgcaca ccggcgatgt cgcggtgcgc gacccgggcg gtttcctgcg
    14941 catcatcgac cgcaccaagg acatgatcgt gaccggcgga ttcaacgtgt atccgcgcga
    15001 gatcgaggac atcatcgcgc aacacccggc ggtgtcgcag gtcgcggtca tcggcgtgcc
    15061 gcaccccagg tggggcgagg cggtgaaggc gctggtggtg ctacgccccg gccagagtgc
    15121 gaccgaggag gaactcatcg ctcccgtcgc cgaggccaag ggctccttcc aggcgcccaa
    15181 gtcggtggan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt catcgctccc
    15301 gtcgccgagg ccaagggctc cttccaggcg cccaagtcgg tggagttcct cgatgccatt
    15361 ccgcagaccg ccgtcggcaa gccggacaag aaggccctgc acgccttgta cgcacagctc
    15421 cgggcctgag cccggccggc actgcgaccg cgttcgcgac ggatcatggc gcggcggccg
    15481 gcagcctgaa ggcgatcagc tcggccccca tgcgcgagag cgcgttgcca ccggcggcga
    15541 tcaccacgaa ctgcgcaccg gaggtgcccc ggtacgtcat cggcaccgcg tgaccgctgt
    15601 agggcaagcg cgtcttccac agttcgctgc catccgcgct ggaataggcg cggaaataac
    15661 cgtccatgct ggccccgatg aagaacaggc cgctggcggt ggcgatgccg ccgccgaagt
    15721 tcggcgcgcc ggtgtcgggg aacagcgccg gcaccggcca cggcgccagg ccctccagcg
    15781 tgccgaaggg aatgcgccat ttcacctcgc cgctgcgcag atccaccgcc gcgaggctgc
    15841 cccagggcgt ggggctgcag ggcgcgccga ggaaggattc cagcggcccg cggcgcgcga
    15901 cgtagggcgt gccgttcatc gggtagacct cgttgggata ctgctgcgcg gcgggatcca
    15961 ggccggcggc ctcctcacgc ggaatcagct gcaccacctg cgcgatgtgg ttctggttca
    16021 cgatcagcaa gccgcgcccg gaatcgatgg ccgcgctgcc ccagttcatg ccgcccgagc
    16081 tgtgcggata accgatcgag cccgcgagcg tcggcggcgt gaaggcaccg tcccagcgat
    16141 accgcgcaat cttgcgcgcg cagtcccggc ggtcgagcgg cgtgaagccg tgcgcatcct
    16201 ccggcgccat cgcgctcggg tgcatcggcg cgggatgcgt ggggaatggc tgcgtgggcg
    16261 acagcgtctc gccgggcacg ccatcctggg gcaccggccg ctcctccacc gggtacagcg
    16321 gcgcaccgct gacgcgatcc agcaggaaga tatggccgac cttggtggtc tgcgccaccg
    16381 ccggcacgcc gccgccgaca ccatcggcct ggaacaggat cggcgcgctc ggggtatcgt
    16441 agtcccacac gtcgtggtgc acgccctgga aactccacac cacctgcccg gtctctatcg
    16501 acaaggccac cgtggaactg ccgtagtgat cgatgccatg gcgctcgccg ccgtagaggt
    16561 ccggcgacgg attgcccgtg ggcacgaaca ccagcccgcg ctcgtgatcc ccggtgatga
    16621 tcgaccagct gttcggcgcg ccggcctgcc acgggctctt gcgcgccgac ggccgcggct
    16681 gccagcccgg cggcaccgga ttccaggccc aggcgagcgc gcccgtgcgc gcatcgaagg
    16741 cgcgcaccat gcccggcgcg gcatcgacct cgaggttgtc ggcaacgaag ccgttgatca
    16801 ccacgcgatc acgcatcacc agcggcggcg aggtggggtg cacggcccag gcgcgcggct
    16861 cgtcgagatc cctgcgcagg tcgacgcggc cgtccgtgcc gaaatcgccg cagggcctgc
    16921 cggtgtcggc gtcgagcgcg agcagttccg agtccgaggt gccgtagaag atgcgcctgg
    16981 cgcagaacgc gcccgccggc gccgcgctgt cctcccagta cgtcacgccg cggcagttga
    17041 gcggataggg cccctcgccc gtgagcgtct tcaccgcggg atcgaactgc cagagttccg
    17101 cgccggtctc gggatcgagc gcgaacacgc gctggaacgg cgtgcagtaa tacagccgcc
    17161 cgtttaccgc gagcggcgtg gcctggaagt acgtcgcgcg cgtcctgccg ctgccgtcgg
    17221 catgatcgcc gctgcggtgc gtccaggcca cttccagcga ggccacgttg gccggcgtga
    17281 tgtcggtgag gcccgagaaa tgcgtggcgc cggcatcgcc cccccatacc ggccactcgt
    17341 tcgcggcgcc agtcgcgggt ccggcgagca gcgaggggct gaaaacggcg agcagcaatg
    17401 cggcgcggcg ggcgccgcgg aaaacggaaa acggcatggg ctggcgaatc ctgcaggtga
    17461 atgtcacggg cgcgaggcgc gggcattgta gtcgagttcg atggccttgc acgcggcgaa
    17521 aacccgcctc aattgccgcg cagcagccgc tcggccagtg tgagcgcctg cgcgatgcac
    17581 tggtccatgt tgtagtactt gtaggtgccc agccggccac agaacagggt gtcggtctcg
    17641 gccgccgcga gggccttgta gcgcgcatac agcgcgcggt tcgcgggatt gggcaccggg
    17701 tagtagggct cggcgtcgtt ccagcacgga tagtcccgcc cgagcgtggt ccagtcgctg
    17761 ctctgaccgt agaaatggcg gtactcggcg ctgcgcgtgt atgcgcgctg atcggggtag
    17821 ttcagcaaac ccacgcgttg caccagttca cggctggcaa tgctctcgag gcggaagtcg
    17881 atgctgcgat acggcaaccg gcccagggca tggtcgaaat aggcgtcgat cgggccggtg
    17941 tagatcagct tgtcgaaacg ctcgctcgcg cgcaccgccc ggtcgtcggc attgagcgcc
    18001 accgtgatgt tctcatgggc cagcatgcgt tcgaacagcc gcgtatagcc ttcgatcgga
    18061 aggccctgcc acctgtcgct gaaatagcgg ttgtcctcgt ggtggcggat cggcaggcgc
    18121 agcgtgaccg aggcgtcgag ctcgcgcggc tccatgcccc attgcttgcg cgtatagccc
    18181 tcgtagaagg cgcggtagag cgcccggccc acctggctga cgatggcctc ctcggcattg
    18241 cggggctgct cgatcggctc gcgcagctcc gccagcagcg cgggcacgtc ctgttcgcgc
    18301 agatcattgc cgaagaatgc cctgaaggtg ctcaggttga gcggcaggtc gtagtagcgc
    18361 ccgccgtgca gcgcctgcac ccgcaactcg tagtcattga acgtggcaaa acgattgacg
    18421 tagcgccaga tcttctcgct gcgggtgtgg aagagatgcg ggccgtaagg accgacgcgg
    18481 atgccatgct cgtccagcct gtcgtaggca ttgcccccga tgtggtcgcg ccggtcgatg
    18541 accagcaccc gcgcgccatg cgcagccagt cgctcggcca gcgtggctcc ggtgaagccg
    18601 cagcccacga tcaggaagtg gtagcgccgc ggcatcagtc gacgtacaac ccgcggcggt
    18661 tgagcagcaa atcctgctgt cgatcgccgc ggcgctcctt gtcccaatgc ggatgcgtga
    18721 gctcgacctc ggccgtatcg atgacggaaa gcgctgcgac gcgcatcccc ggagcacacg
    18781 ccaggatcga aggcacgctg acctcgcaat agccgctcac gcctgcgcgg tacacgctga
    18841 caaggtggtc gagcgcacgc ggtgtgatgc ggatgcaggg aaagaagccg cccaccgtgg
    18901 cagcgtgacc gtcgagcttg tcccaccaga accagtcggg ctgcaggatg cacgcgctgt
    18961 gcaccagtgc gcgcaagcca cgctcgcgga tcttcagccc gggcaccagc aggtcgaagg
    19021 gaagatcgcg tcccaggaaa tcccagctgc cgctgcgcag actgacgtcg tactcgatct
    19081 gccacagcgc cgtgtagcgc tgcggatgat gcaggtggaa gtcgatcagg gaatagtcgg
    19141 agtgatacca gtcgagcggt gcgcccatcc cggcaaggaa cgcatgcggg cgcaggccgt
    19201 ggtcggcgta gcgatcacga tggaatccgt gcaggcgcag ccccggcagg gccgcttcga
    19261 ccgccgccag cgcgaccgcc ggcacggccg cggcatcgac accgagaacc agttccaccc
    19321 ggggctggcc gagcaaggca cggcgcatcg tgcgcagctg tgccagcagc gcatcgtcga
    19381 tgaaatgggt cttgaccaga acggcgatca cgggattcag ctgggctccg
//
LOCUS       DAORUQ010000035        19080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2505_length_19080_cov_6.693035, whole genome shotgun sequence.
ACCESSION   DAORUQ010000035 DAORUQ010000000
VERSION     DAORUQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19080
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_19080_cov_6.693035"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..1636
                     /locus_tag="PLW13_05055"
     CDS             482..1636
                     /locus_tag="PLW13_05055"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY69771.1"
                     /translation="MAQQQLVADELIGEARRATGLERFDSDSFREGLEIFVADVNGSR
                     PPQAAIERLRGAVVAALAARLKTTAYLEQRPELLQRPIERPVFVFGIPRTGTTLLSNL
                     LAADPARRSPLTWEIDDPVPPPAKDTLYTDARALARLESERQMLAARPEMGRYYRGSA
                     IYPNECMFFMQHDFKALIWESRGKLPNYRDWLFQADVSSTYRYHKRFLQLLQADAPGN
                     WNLKMPSHGLWIQTLLAVYPDARLIWTHRDPLTATGSFCSIIALSHQAFTAKVDLEWL
                     GENCSWQALQHAERIMDARDALGEERIIDVHYANLARQPIETMRALYRALGDEFTAQA
                     EAGMRAWLADNPQDKFGRHEYKLAQYGLSPSKVEALLERYLSRYEVEREG"
     gene            complement(1659..2213)
                     /locus_tag="PLW13_05060"
     CDS             complement(1659..2213)
                     /locus_tag="PLW13_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY69772.1"
                     /translation="MNGGFGAVMQHGYIVTNAAAAAVEWAQRVGAGPFYVIDRMPMDQ
                     YFYRGVRTELELRLAFGYWEGLQIELIEPLGSADSLYSRALRDAPGKLNHCATVVSDI
                     DRLLAQHGLHDRVIQSGKMPSGLKFVYLDEYLPGGLHLELIEANEGMLAGFAGMQAVS
                     RQWDGQDAVRPITRLQEDLAGLRQ"
     gene            complement(2241..2588)
                     /locus_tag="PLW13_05065"
     CDS             complement(2241..2588)
                     /locus_tag="PLW13_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69773.1"
                     /translation="MSTQSSLLPAGFDALAPFVEYWAADTAAGRARCRDLSDEASRAA
                     FYDAANDLVPKALACLDERPLDRLDESEQRLMRLVLSFAHVAMAVELQREEEPRHARY
                     RPFMRITRAPADL"
     gene            complement(2585..3787)
                     /locus_tag="PLW13_05070"
     CDS             complement(2585..3787)
                     /locus_tag="PLW13_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY69774.1"
                     /translation="MNTPVTGPGAPPGEEALALGTGPVPAGPYYRPEYFELEREAIFR
                     RTWLNIGHVCELPAAGSFIVRQLDFANASILITRGKDGVVRAFHNVCTHRGTQLVAEQ
                     DGLKSTFTCPYHAWTFSNDGQLRAAPDFERFYVDKAQCSLPAVAVDVCAGLLFVNLDP
                     SPKQGLREYLGPFVEQWETLPVGDATTFSEYVYEIDANWKLTYDNFQENYHLRFIHPR
                     SGGGGAVGPDNPFGYPMDYKFYGEHRTQTIWTNPAPKLSPAQMVAFGKLVPALMQRGL
                     MNHPYGRAYWAVFPNLFMIGTPTQPFVHYVMPISATRSRGVIRIYWVGEDMNASERYG
                     REFSMAMARDVHSEDRVVIEAGQRGLNSGALEHIHLQQQEVLLRHLMYHVDAKVQAYK
                     AEQAGGAA"
     gene            4014..4625
                     /locus_tag="PLW13_05075"
     CDS             4014..4625
                     /locus_tag="PLW13_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY69775.1"
                     /translation="MKSPDATARAGTPARRRHKCFDDTHREIIETTVRLISEKGVDAV
                     SIAAVARAMGINRATVYYHFESLGALIGAVKAWSSEQLAKPFTNAAPQQERVDYITRF
                     VLENPELMKLWIEDLISVGDIRTRYAHWDALVEGIRGHDLTSGPGEAVDAEVFCVILL
                     TSSIIGPRVFRNSVCPGADTETVVKRFRNEQLRMLRHAALLKL"
     gene            4729..5595
                     /locus_tag="PLW13_05080"
     CDS             4729..5595
                     /locus_tag="PLW13_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY69776.1"
                     /translation="MERAVDDVMTIEAELRVPVATAQLVRFHFAEPADNLFCQDRYQL
                     DLCLTPRPRNARACFPDRWSPHRFERIGALFMVPPGEAMRARSDDCGQQTSIICQLHP
                     ESLRDWFDGELQWDARRLMAGLDIREANIRSLLLRLAEEARNPGFASDVLVELIATQL
                     AIELARYCTALDDEARVRGLASWRLRLVDERLREEGRIPTLAELAALCGLSVRQLTRG
                     FRTSRGCSIGEYVESSRVAHARRLLATGQSVKAIAYALGFASPSGFCFAFRRATGETP
                     REFRQRLGRSAH"
     gene            complement(5635..6159)
                     /locus_tag="PLW13_05085"
     CDS             complement(5635..6159)
                     /locus_tag="PLW13_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY69777.1"
                     /translation="MSIDQTALATLIAKEEIRELVLLYSRGVDRKDGALLRTLYTRDA
                     TDTHGDTFDGAAEDYVQFLERAFPYMRYSGHHVCNHLISISGDEGEGEVYAIAYHIIP
                     DGKDGWIEDLMCARYIDRYRKEDGRWRFAKRVVTYDMRSQHPFPANGSTAAQLDDPGF
                     GALHSRLFARGPRA"
     gene            complement(6208..7011)
                     /locus_tag="PLW13_05090"
     CDS             complement(6208..7011)
                     /locus_tag="PLW13_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69778.1"
                     /translation="MIGEDPLDLGGQVAFVTGAGQGAGRAIALALARHNAGGVAVNDF
                     VAERAEAVAAEIRALGVAAVAAPADVGDHASVQSAMTTAAAALGPITLLVNNAGNAGP
                     DVQMRASPLFWETAPQEWDRYFRTNLQGVMNCCHVALPGMVAQGKGRIVTIVSDSGRI
                     GEAKLVAYSAAKAGAAGFVRSIAREAGRFGITSNAISLSTLEPPLPEPARTEFLASER
                     AKAHVARYIIRRFGQPDDVANMTLFLCSDAASWITGQTYPVNGGYSFAQ"
     gene            7275..8237
                     /locus_tag="PLW13_05095"
     CDS             7275..8237
                     /locus_tag="PLW13_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQY69779.1"
                     /translation="MIEDRPAPGPLGPGQVRIAMRAASINYRDFLALSGAIGVRGPAG
                     LIPCSDGAGEVIETAPDVHRLRVGDRVALAFNPDWIGGPWQATTGVLCRGSGLPGVMQ
                     EELVVHHGEAVVLPAHLDFGEGAALPCAGVTAWHALCGATPLLPGMSVLLQGGGGVSV
                     FALQFAKLFGARVIMTSSGPQRCARLRSLGADETIDYVAQPQWDAAVRALTGGHGVDL
                     TVEVGGATTIDRSVAATRRGGRVALVGLLTGVPAAVPSLFSAGASISPVMVGSRDDFE
                     AMNRAIAFHRLRPVIDSRYGFEQLPDALRHLQSGRHMGKIVIDF"
     gene            8291..9088
                     /locus_tag="PLW13_05100"
     CDS             8291..9088
                     /locus_tag="PLW13_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY69780.1"
                     /translation="MEKDFTVRIAGADFYARQQGDKPRAVFLHGFGGDLHTWDGVWSE
                     LACAVPALRYDLRGFGRSASHDDAPFDHADDLLAILDATGIGQCDLVGVSMGAGIALN
                     FTLDHPGRVRNLVLISPGLVGWEWSADWQSLWRPIVAAARAGAIDEARRLWWQHPLFA
                     STRSSAGGQALFDSIMRYCGAHWIRDNHRLKLPDVERLCLLGTRTLLLTGGRDLDDFR
                     LIADLVAASARAVRRVDLPGAGHLLHLEDPAGCARELLAFLRPARAP"
     gene            complement(9104..10579)
                     /locus_tag="PLW13_05105"
     CDS             complement(9104..10579)
                     /locus_tag="PLW13_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQY69781.1"
                     /translation="MDDLEQLSAAALARPAQQPAIEFQKQWTSWGAMRRVGERVGELI
                     VASGAGPGARVAFVARNRPSALAALLGMIAGRSTIRMVYPFQSATAIARDLDAIEPSV
                     LVAAAEDFPETILAVLRSRGIAAIALREMEAWSLPGLERARRPGDATLPPQVEILTSG
                     TTGPPKPFRLSYATIARFIVAPRAMDSGDPALAPPALLYFPVGNISGLYSTLPALLNG
                     QRAVLLDRFSLAGWHDHLLRFRPAMGGLPPAGFQMVLDADIPRADLGCLRYMGAGAAP
                     LAPEVQRAFEERYGVPILLSYGATEFAGPVAAMTPELHATWGRSKLGSVGRALPGAQL
                     RVVDPASSAVLPPSAEGILEVISPRVGPDWIHTSDVAVIDADGFVFLRGRADGAIIRG
                     GFKLLPETIERALLLHPGVSAAAVIGLADKRLGQVPAALVQLAAESPQTGVAELEAHL
                     RDHVPATHIPVVWRFVDALPRTAMSFKVDRPAVRKLLEDAD"
     gene            10659..11882
                     /locus_tag="PLW13_05110"
     CDS             10659..11882
                     /locus_tag="PLW13_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY69782.1"
                     /translation="MNTNTVTVIIGAGQAGCDLATALRQQGYAGRIILLGEEPVPPYR
                     RPPLSKTYLSGDIGEEALYIKPRAAYAKQDIDLRTGVRVTAIDRQAHTVSLDNGERIA
                     YDKLAITTGGRPRQLPLAGADKPNVHYVRTLEDINRLREQFEPGRRLVIIGGGYIGLE
                     AAAVGIKKELAVTLVEALPRLLARVTGPELSQYYAGVHRRHGVDIRLGAGVEALEGGE
                     QVEFVVLQDGTRLPADVVIVGIGLVPNTELAEQAGLEVDNGIIVDLYAQTSDPDIVAA
                     GDCTNHDNGFLGRRLRLESVPNASEQGRVAAASICGQRVAHAGVPWFWSDQYDLKLQM
                     VGLSQGHDRVVIRGSMEADSFAAFYLQDGVVIAVDAVNRPQDFMVGKKMVAARLKADP
                     ATLADESVVLKSLVA"
     gene            11904..13184
                     /locus_tag="PLW13_05115"
     CDS             11904..13184
                     /locus_tag="PLW13_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY69783.1"
                     /translation="MTPTAYARVLLGSLGREELQPAEDKRGAALLWAQSGAMWLSGHA
                     DGPARSCPAPLAACAQGAWLALSALRPQHCDPRFDAYRLLGERAAIAGLGRRGRVSAG
                     GACHLLDTADGALALNLARDDDHDLLPAWLEQPVAGMGELAEILRARPTVALVQRARL
                     LGLAAAALVAPRPRRTWFHARRHAPARRDGRAAPLVIDLSSLWAGPLCAQLLAGCDAR
                     VIKVESTARPDGARRGPAWFYDLLNAGKESVALDLREPADRGRLHALLAQADIVIESA
                     RPRALEQMGIHAAQLLAARPGMVWLGITGYGRRAPMRDWIAYGDDAGVAAGLAWLMGG
                     ARGDPVFCADAIADPLTGLHAAALALAAWLDGGGQLLDIALHDVAAFCAGAGCESDPG
                     AVPLVEAALPLARVACEQAAEPGVDTARVLREFT"
     gene            13197..14654
                     /locus_tag="PLW13_05120"
     CDS             13197..14654
                     /locus_tag="PLW13_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69784.1"
                     /translation="MSVTEAGSRDDASLLIRRALLPQRGLFDLRIGGERIVALQRELE
                     PCAGERIIDARGNVLLPGLHDHHLHLFATAAARASLLCGPPAAHEEQSLRQALARHCA
                     QGEGWVRGIGFHESACAQLDRHWLDAVCAERPVRIQHRSGMLWVLNSCALGHLRVAPD
                     ECLPAGAERTADGELTGRFFDLDAWLGSRLPRAWPSLRDVSAELARHGITAVTDAGAR
                     NGQAAWEALCGASARGELVQRVLVMGNEELAVSAGASPGRIEVGPLKIYLREIGLPEF
                     EALVLRVQAAHAQGRAVAFHCVTRVELTFALAALAQAGTLPGDRMEHAAIADEHALQA
                     LAALGVTVVTQPQFIAERGDQYLRDVEAVDIPLLYRGAGFLRGGVALAAGSDAPYGST
                     DPWAAMRAAIGRRTRAGVQMNAGECLAPAQAIALFAGDLHRPASGLRELAVGQPADLC
                     LLDVSWEDLCADPDARHVALTVCAGRIVYAAPRHA"
     gene            14647..15555
                     /locus_tag="PLW13_05125"
     CDS             14647..15555
                     /locus_tag="PLW13_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY69785.1"
                     /translation="MHRAIIASVDELPQDPQWLGSRPCPVIAYGAGAASASADVLVPD
                     LAAARAIEKRVLAWPVAAMTLVQVLRSTALLPAAQALDVESMAFATLQGGEEFAAWQR
                     SARRGPPPLPVQAGDPVLLSREGAVIEAVLNRAGTRNAISVEMRDAWVEALQLLEADE
                     SVEELRFRALGACFSVGGELHEFGLAPDPATAHWVRTVHSPARLLARLGTRVSFFVHG
                     ACIGSGIELPAFAARLVAHPETFFQLPELQMGLIPGAGGTVSIARRIGRQRTAWMVLT
                     GRRISARTALEWGLVDALVDVSSAPP"
     gene            15689..16948
                     /locus_tag="PLW13_05130"
     CDS             15689..16948
                     /locus_tag="PLW13_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY69786.1"
                     /translation="MKTTLIRNATIWDGSGARAFAGDLLIEGNRIRSVTPGDAEAGAR
                     ADVQIDAAGRFLMPGMTEGHAHLSFDGVTCTENLITPPPEEHTLLTARVAKTLLDHGF
                     TSAYGASAAKLRLDVAVRNEIDAGRLPGPRVRAGSLEITVTGGLGDESKLHNPRNGPS
                     IVVDGAEEMRKAVRLCCREGCDNIKLDVSGDPFYPEIPSHATPMSLEEVRMAVATAHS
                     FGKKVNAHARSIESVRHCLVAGVDVIYHAEYSDEETLDMLEAAKDRIFVAPTISLFHT
                     MMHEAEPYLPRAAAEAMGIPALIEESVKTHTALRKRGIRHLIGGDYGFAWSMQGTNAR
                     DLKFFMDYYGYSAADALVCATRNGGLAMRAQGDLGTLRPGMLADMLLVDGDPLTDVTI
                     LTDRRRLAMVMKDGEISTLQPALHATG"
     gene            complement(16970..18817)
                     /locus_tag="PLW13_05135"
     CDS             complement(16970..18817)
                     /locus_tag="PLW13_05135"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY69787.1"
                     /translation="MRRLPLALLSCATLLLAEAGAAAAANTAAPAARQVLYGDLHIHT
                     ALSADAFSTGTRTLPDDAYRYARGEAIAHAAGFTVQLKRPLDFAAVTEHSEYLGSMRE
                     FLNPASPLSQHPLAKLFASPDPADAFKALMQISDDASHDGSLDELLDLPEVNMAAWRE
                     VIAAAERHNEPGRFTTLIGYEWTSTPDEINLHRNVIFRGSAVPPKPFSRIDSDKPEDL
                     WRFLDEQRAQGIEGMAIPHNSNASDGMMWARTDSAGRPVDAAWARQRSRNEPVAEIVQ
                     IKGQSEAHPLLSTEDEFANYEMWTTRMSGAKIPRASKPEGSYARDALKTGLELGASLG
                     VNPYDFGMIGSSDTHNSAPPIEEDNYHGKLTQLDGTAAMRAGRIARSAEARAAGRETA
                     YSAAGLAAVWADANTREAIYDALRRKETFATSGTRISLRFFGGWDFAPALVQAPDFVA
                     QAYASGVPMGSQLPAPAARGAPRFAVLATKDPDGANLDRVQIVKGWLDVDGTAREKIF
                     DVAWAGERAIDAASGKLPAVGSTVDVKTATYRNTIGAAELSAVWQDPEFQPQARAFYY
                     VRVLEIPTPRWSTYDAVKLGTAAQEPAAIQERAWSSPIWFAPAAATPGS"
BASE COUNT         2930 a         6317 c         6689 g         3144 t
ORIGIN      
        1 gttcttgtcg gcgttgcgcg cccagacggc gacatcgccg cccatcctgg ccacgcccat
       61 ggcaaaaccc agaccgatac cgccgttgcc gccggtgacc agcgtgacct tgccggaaca
      121 atcgaacagt cccttactca tcgcgcgttg ctcctgttgt gccggcagcg gatgctggtg
      181 ttttcccgga ttcatccgcg ctagtttccg ccacccgggc gcgcccttca acagaaagga
      241 atgtcgacgg cgggccgcaa tggtgcaggc gggacatgac gcgtttgcgc gcgcaccggc
      301 ccgcgcgctc cttgccctcg cgcggcgggg cgatggccgg acgccgatat tgtgtcccgc
      361 gctcgaagtc gaatgacgtt gccagctcgc ggcgacgcgc accaatatgt ctgcttcagg
      421 cagggtcggc aaatcgcccc cgggttggcc cgctacggcg atgaacagga ggtcgaggga
      481 tatggcgcag caacaactgg tcgcggacga attgatcgga gaagcgcggc gcgcgacggg
      541 gctggaacgc ttcgacagcg acagcttccg cgaggggctg gaaatcttcg tggccgacgt
      601 caacgggagc cggcctccgc aggcggccat cgagcggctg cgcggcgctg tcgtcgctgc
      661 ccttgccgct cgactgaaga ccacggctta cctggagcag cgccccgagc tgttgcagcg
      721 tccgatcgag cggccggtgt tcgttttcgg cattccgcgc accggcacca cgctgctcag
      781 caacctgctc gcggccgatc cggcgcgccg ctcgccgctg acttgggaaa tcgacgaccc
      841 ggttccgccg cccgcgaagg acacgctcta caccgacgcg cgcgcgctgg cgcggctgga
      901 gtcggagcgc cagatgctgg cggcgcgtcc ggagatgggc aggtactacc gcggctcggc
      961 gatctatccc aacgagtgca tgttcttcat gcagcacgac ttcaaggcgc tgatctggga
     1021 gtcgcgcggc aagctgccga actaccgtga ctggttgttc caggccgacg tcagctcgac
     1081 gtaccggtat cacaagcgtt tcctgcagct gctgcaggcg gatgcgccgg gcaactggaa
     1141 cctgaagatg ccttcgcacg ggctgtggat ccagacgctg ctcgcggttt accccgatgc
     1201 ccggctgatc tggacccatc gcgatccgct cacggccacc ggctcgtttt gcagcatcat
     1261 tgcgctgtcg caccaggcgt tcaccgcgaa ggtcgacctg gaatggctgg gcgagaattg
     1321 ttcctggcag gccctgcagc acgccgagcg catcatggac gcgcgtgatg cgcttggcga
     1381 ggagcgcatc atcgacgtgc actacgccaa cctggcgcgc cagccgatcg agaccatgcg
     1441 cgcgctctac cgggccctcg gtgacgagtt cacggcgcaa gcggaggcgg ggatgcgcgc
     1501 ctggctcgcc gacaacccgc aggacaagtt cggcaggcac gagtacaagc tggcgcaata
     1561 cgggctgagc ccgtcgaagg tcgaggcgct tctcgagcgc tacctgtcgc gctacgaggt
     1621 ggagcgggag gggtgagcgc ggcagcgccg cgaagcggct attgccgcag gccggccagg
     1681 tcttcctgca ggcgcgtgat cggccgcacc gcatcctggc catcccactg ccggctcacc
     1741 gcctgcatgc cggcaaatcc tgccagcatg ccttcgttgg cctcgatcag ttccaggtgc
     1801 aatccgccgg gcaggtactc gtccaggtag acgaacttca ggcccgaagg catcttcccg
     1861 gactggatca cgcgatcgtg cagcccgtgc tgcgccagca agcggtcgat atcgctcacc
     1921 accgtggcgc agtgattgag cttcccgggt gcgtcgcgca aggcgcggct gtagaggctg
     1981 tcggcagacc cgagcggctc gatcagttcg atctgcaggc cttcccagta gccgaaggcg
     2041 aggcgcagct ccagctcggt gcgcacgcca cgatagaagt actggtccat gggcatgcga
     2101 tcgatcacgt agaacggccc ggcgcccacc cgctgcgccc attcgaccgc tgccgcggcc
     2161 gcatttgtga cgatgtagcc gtgctgcatc accgctccga aaccaccgtt catcggcatc
     2221 actcctggaa attgcgggat ctagagatcc gcgggtgccc gcgtgatccg catgaacgga
     2281 cggtacctgg cgtgcctggg ttcttcctct cgctgcaatt cgaccgccat cgccacgtgg
     2341 gcgaagctga gcacgagtcg catcagtcgc tgttcgctct cgtcgagtcg atccagcggc
     2401 cgctcgtcga gacaggccag cgccttcggc accaggtcat tggccgcgtc gtaaaacgcc
     2461 gcgcgagagg cttcatcgct gaggtcgcgg cagcgcgcac gcccggccgc cgtgtcggca
     2521 gcccagtact ccacgaacgg cgccagcgcg tcgaacccgg cgggaagcag tgacgactgc
     2581 gtgctcacgc cgcacctccg gcctgttcag ccttgtaggc ctggaccttc gcatcgacgt
     2641 ggtacatcag gtgccgcagc agcacttcct gctgctgcag gtgaatgtgc tcgagtgcgc
     2701 cgctgttcag gccgcgctgc cctgcctcga tgaccacgcg gtcttccgaa tggacgtcgc
     2761 gcgccatcgc catcgagaac tcgcgcccgt agcgctcgct tgcattcatg tcctcgccca
     2821 cccagtagat gcggatgaca ccgcgcgatc gcgtcgccga gatcggcatg acgtaatgca
     2881 cgaagggctg ggtcggcgta cctatcatga agaggttggg aaaaaccgcc cagtacgcgc
     2941 gtccgtacgg atggttcatc agccctcgct gcatcagggc cggcaccagc ttgccgaacg
     3001 cgaccatctg cgccgggctc aacttcggcg cggggttggt ccagatcgtt tgcgtgcgat
     3061 gctcgccgta gaatttgtag tccatcggat agccgaacgg gttgtccggc cccaccgcgc
     3121 caccaccgcc cgagcgcgga tggatgaagc gcaggtgata gttctcctgg aagttgtcgt
     3181 aggtcagctt ccagttggca tcgatctcgt aaacatactc cgagaaggtc gtggcatcgc
     3241 cgaccggcaa ggtctcccac tgctcgacga aggggcccag gtattcgcgc aggccctgtt
     3301 tcggtgaggg atccaggttg acgaagagca ggcccgcgca cacgtcgacg gcaacggccg
     3361 gcaggctgca ctgggccttg tcgacgtaga agcgctcgaa atcgggcgct gcgcgcaact
     3421 ggccgtcatt gctgaaggtc caggcgtggt aggggcaggt gaacgtcgac ttgagcccgt
     3481 cctgctccgc aaccagctgg gtgccgcgat gggtgcagac gttgtggaag gcgcgcacga
     3541 caccatcctt gccgcgggtg atcaggatcg aggcgttggc aaaatcgagt tgccggacga
     3601 tgaagctgcc ggccgcgggc aactcgcaga catgcccgat gttgagccag gtcctgcgga
     3661 agatcgcctc gcgctccagc tcgaaatact ccgggcgata gtaaggaccg gccgggactg
     3721 gtcccgtgcc cagcgccagc gcctcctccc ccgggggcgc tcccggtccg gtgactggtg
     3781 tgttcattgg ccgcatcccc ccgccgacag gctggttccg aagacaagtg gccgcgcacg
     3841 accccgactt gcagcccggc aggcaaccgg gcttccgtgc atcgcgttgc aaaattacga
     3901 catatgtgtc gtaatttcaa cagtagtgtt gaatctttta tgtgacggga tgaacgggga
     3961 gatatcgcgt gagtgttcct gcaggcaagc ccggtcgcac aacggtcgtt gtcatgaagt
     4021 ctcccgacgc gacggcgcgt gccggaactc cggcgcggcg acgccacaag tgtttcgacg
     4081 acacgcaccg cgaaatcatc gagacgacgg tccgcctgat ctcggaaaaa ggagtggacg
     4141 cggtgtcgat agccgcggtg gcacgcgcga tgggcatcaa ccgcgcaacc gtctactacc
     4201 acttcgagag cctgggggcg ctgattggcg cggtgaaggc ctggtcgtcc gaacagctgg
     4261 cgaaaccgtt caccaacgct gccccgcagc aggagcgggt cgattacatc acccgcttcg
     4321 tgctggagaa tccggagctc atgaagttgt ggatcgagga cctgatctcg gtcggcgata
     4381 tccggacccg atacgcgcac tgggatgcgc tcgtggaagg catcaggggt cacgacctca
     4441 cgagcggccc tggcgaggcg gtcgacgcgg aggtcttctg cgtgatcctg ctgaccagct
     4501 cgatcatcgg tccccgtgtt ttcaggaaca gtgtctgccc cggcgcggac accgaaacgg
     4561 tggtgaagcg attccggaac gagcagctgc ggatgctcag gcacgcggcc ctgctcaagc
     4621 tctgaggccc ggatccggtc cggcaagccc cggatgcgac ggtcgacaat gacattttct
     4681 gcgcgcaggc cacgtctggc cggcacgacg agcgagggca cagacgggat ggaacgcgct
     4741 gttgacgacg tgatgaccat cgaggcggaa ctgcgcgtgc cggtggcgac agcgcaactc
     4801 gtgcgtttcc attttgccga gccggccgac aacctgttct gccaggaccg gtatcaactg
     4861 gatctctgcc tcacgccgcg accgcgcaat gcgcgcgcct gcttccccga tcgctggagc
     4921 ccgcatcgat tcgagcgcat tggtgccctg ttcatggtgc ctcccggcga ggccatgcgt
     4981 gcccgcagcg atgactgcgg ccagcaaacc tcgatcatct gccagctgca cccggaatcg
     5041 ctgcgcgact ggttcgacgg cgaactgcag tgggatgcgc gccgcctgat ggccgggctc
     5101 gatatccggg aggcgaatat tcgcagcctg ctgctgcgcc tggccgagga ggcgcgcaat
     5161 ccgggatttg ccagcgatgt gctggtcgaa ctgatcgcga cgcagctggc catcgagctg
     5221 gcccgttact gcaccgcgct ggacgatgag gcgcgggtgc gcggcctggc gtcctggcgg
     5281 ctgcggctgg tcgatgagcg gctgcgcgaa gagggcagga taccgacgct ggccgagctg
     5341 gcggcgcttt gcggcctgtc ggtgcggcag ctgacccggg gctttcgcac cagccgcggc
     5401 tgctccatcg gcgagtacgt ggaaagcagc cgcgtggcgc atgcccggcg gctgctggcc
     5461 accgggcaga gcgtcaaggc gattgcctac gcgctgggct ttgcctcacc gtcgggcttc
     5521 tgcttcgcgt ttcgccgcgc gaccggggaa acgccgcgcg agtttcgcca gcgcctgggg
     5581 cgcagcgcac actgatcccg agtgtgccag ccccgcgctg cgtggcgcag gctcctaggc
     5641 gcgcgggccg cgggcgaaca ggcggctgtg cagcgcgccg aagcccggat catcgagctg
     5701 cgcggcggtc gaaccgtttg ccgggaacgg gtgctggctg cgcatatcgt aagtgactac
     5761 acgtttggcg aaacgccacc ggccgtcttc cttgcggtag cggtcgatgt agcgggcgca
     5821 catcagatcc tcgatccatc cgtccttgcc gtcgggaatg atgtggtaag cgatggcata
     5881 gacctcgcct tcgccctcat cgccgctgat ggagatcagg tgattgcaga catggtgacc
     5941 gctgtagcgc atgtacggga aggcgcgctc gagaaactgg acatagtcct cggccgctcc
     6001 gtcgaacgta tcgccatgcg tatccgtggc gtcccgggtg tagagcgtgc gcagcagtgc
     6061 gccgtccttg cgatcgacgc cgcgcgagta gagcaggacg agttcgcgga tttcctcctt
     6121 cgcgatcagc gtcgcgagtg cggtctggtc gatgctcacg atagtcctcc ggtgtctgga
     6181 cgggatgttg cgttcagccg gcggctgtca ctgggcaaaa gagtacccgc cgttgacggg
     6241 ataggtttgc ccggtgatcc acgaggcggc gtccgaacac aggaagagcg tcatgttggc
     6301 gacgtcgtcg ggctgtccga agcggcgaat gatgtagcgc gccacgtgcg ccttggcgcg
     6361 ttcgctggcg agaaactcgg tccttgccgg ctcggggagt ggcggctcca gtgtcgagag
     6421 agaaatcgcg ttcgaggtga tgccaaagcg ccccgcctcc cgggcaatgg aacgcacgaa
     6481 gccggcggcg ccggccttcg ccgccgaata ggcgacgagc ttcgcttcgc cgatgcgccc
     6541 ggagtccgag acgatcgtga cgatgcggcc cttgccctgc gcgaccatgc ccggcagtgc
     6601 cacgtgacag cagttcatca cgccctgcag gttggtgcgg aagtagcggt cccattcctg
     6661 cggcgcggtt tcccagaaca gcggcgaggc acgcatctgg acgtcgggtc cggcattgcc
     6721 ggcattgttc acgaggaggg tgatggggcc gagcgccgcg gccgcggtgg tcatggccga
     6781 ctgcaccgag gcgtggtcgc ccacgtcggc gggcgcggcg acggccgcca ccccgagcgc
     6841 cctgatctcc gccgccacgg cctcggcgcg ctcggccacg aaatcgttga ccgccacgcc
     6901 gcccgcgttg tgacgcgcca gtgccagcgc gatggcgcgc ccggcgccct ggcccgcgcc
     6961 ggtgacgaat gcgacctggc cgccgaggtc cagcggatct tctccaatca tgtcaaatct
     7021 ccttgctccg gattctctgc gcggcatgcg tgacactgcg cggttgccgc cctggcgccg
     7081 cgggcgagca cgcgggtcga gccggccaac acggcccgat cgattgactc gcaactgaag
     7141 attcagcata ttcggaacat gctgaatacg tcaaaacaaa gcgttttcgc cgaacacttt
     7201 cgcccggtcg atgacgtggc gatgcagcga tgaaaacctt tgtctcgcgc acgcccgagg
     7261 gcgtggattc cctcgtgatc gaggaccggc cggcgcccgg ccccctcggg ccgggacagg
     7321 tgcgcattgc catgcgggcc gcgtcgatca actatcgcga ctttctggcc ttgtccggtg
     7381 cgatcggggt gcgcgggccc gccgggctga tcccgtgctc ggacggcgcg ggcgaagtca
     7441 tcgagaccgc gcccgatgtg catcgactcc gggtcggcga tcgcgtggcg ctcgctttca
     7501 atcccgactg gattggcggc ccgtggcagg ccacaaccgg cgtgctgtgc cgcggcagcg
     7561 gcctgccggg cgtgatgcag gaggaactgg tcgtgcatca cggcgaggca gtggtgttgc
     7621 cggcacacct ggatttcggg gagggggctg ccctgccatg cgcgggcgtg acggcgtggc
     7681 acgcgctgtg cggcgcgaca cccttgctgc cgggcatgtc ggtattgctg caagggggcg
     7741 gtggtgtctc ggtgttcgcc ttgcagtttg ccaagctgtt cggcgcgcgc gtgatcatga
     7801 cctcgtccgg cccgcaacgc tgcgcgcgcc tgcgcagtct cggtgccgac gagaccatcg
     7861 actacgtcgc gcaaccgcag tgggacgcgg cggtgcgggc gctcaccggc gggcacggtg
     7921 tggacctgac ggtggaggtc ggtggcgcga cgacgatcga tcgctcggtg gcagctacgc
     7981 gcaggggcgg ccgcgttgcg ctcgtgggtc tgctgaccgg ggtgcccgcc gcggttccat
     8041 ccctgttttc cgccggtgcc agcatcagcc cggtcatggt cggcagccgc gatgatttcg
     8101 aggcgatgaa ccgtgccatc gcatttcacc ggctgcgacc cgtgatcgac agccgctacg
     8161 gcttcgaaca attgcccgat gcattgcgcc atctgcagag cggccggcac atgggcaaga
     8221 tcgtcatcga cttctagcca tctgcggcgc gatcgtcggc ccgagccctg tcgtcacggg
     8281 ggagcgcaac gtggagaagg atttcacggt ccgtatcgca ggtgcggatt tttacgcgcg
     8341 ccagcagggc gacaagcccc gcgccgtctt cctgcacggc tttggcggcg atctccacac
     8401 ctgggatggc gtgtggtcgg agctggcctg cgctgtcccg gcgctgcgct acgacctgcg
     8461 cggtttcggc cgctcggcga gccacgatga tgccccgttc gaccacgccg acgatctgct
     8521 ggccatcctc gacgcgacgg gcatcgggca atgcgacctg gtcggcgtgt cgatgggcgc
     8581 cggcattgcg ctgaacttca cgctcgatca ccccgggcgg gtgcgaaacc tggtcctcat
     8641 cagtcccggt ctggtcggtt gggaatggtc cgcagactgg cagagcctgt ggcgccccat
     8701 cgtcgccgcg gcgcgcgcgg gggcaatcga cgaggcccgg cgcctgtggt ggcagcatcc
     8761 gctgtttgca tcgacccgca gcagtgccgg cgggcaggcg ctgttcgact cgatcatgcg
     8821 ctattgcggc gcccactgga tccgcgacaa ccaccggctg aagctgccgg atgtcgagcg
     8881 gctctgcctg ctcggcacgc gcacgctgct gctgacgggt ggccgtgacc tggacgattt
     8941 caggttgatt gccgacctcg tcgcggcaag cgcccgcgcg gtgcggcgtg tcgatctgcc
     9001 cggtgcgggt cacctgcttc acctggagga tccggcaggc tgcgcccgcg agctgctcgc
     9061 gttcctgcgc ccggcccgcg cgccctaggc ttcacggcgg cgatcagtcg gcgtcctcga
     9121 ggagtttgcg cacggccggt cgatcgacct tgaacgacat ggccgtcctc ggcagggcgt
     9181 cgacgaagcg ccagaccacg ggtatgtggg tcgcgggcac gtgatcgcgc agatgcgcct
     9241 cgagttcggc gacaccggtc tgtggcgatt ccgcagcgag ttgcaccagg gctgccggta
     9301 cctggcccag gcgcttgtcc gcgagcccga tcacggctgc tgccgacacg ccggggtgca
     9361 gcagcagcgc gcgctcgatc gtttcgggca gcagcttgaa accgccgcga atgatcgccc
     9421 cgtcggcgcg gccacggaga aacacgaagc cgtccgcgtc gatcaccgcg acgtccgagg
     9481 tatggatcca gtcgggaccg acgcgtggcg agatcacttc caggatgccc tccgcgctgg
     9541 gcggcaggac ggcgctactg gcgggatcga cgacgcgcag ttgcgccccc ggcagggcac
     9601 gccccacgct gccgagcttg ctcctgcccc acgtggcatg gagctcggga gtcattgccg
     9661 ctaccgggcc ggcgaactcg gtggcgccat aggacagcag gatgggcacg ccgtagcgct
     9721 cctcgaaggc ccgctgtact tccggggcca ggggcgcagc ccccgcgccc atgtagcgca
     9781 ggcagcccag gtcggcgcgc gggatgtcgg catccagcac catctggaag ccagccggcg
     9841 gcaaaccacc catcgcgggg cgaaagcgca gcaggtgatc gtgccagccg gccaggctga
     9901 agcggtccag cagcaccgct cgttgcccgt tcagcagcgc cggcaacgtg gaatagaggc
     9961 cggaaatatt gcctaccggg aaatacagca gggccggggg cgccaatgcc ggatcgccgg
    10021 aatccatcgc gcgtggagcg acgatgaacc tggcgatcgt tgcgtagctc agccggaacg
    10081 gcttgggcgg accggtggtg ccgctggtca ggatttccac ctgcgggggg agcgtggcgt
    10141 cgcccgggcg ccgcgcgcgc tccaggccgg gcagcgacca ggcttccatt tcccgcagtg
    10201 cgatcgcggc gatgcccctg gagcgcagca cggccaggat tgtctccggg aaatcctcgg
    10261 cggcggctac cagtaccgag ggctcgatcg cgtcgaggtc gcgcgcaatg gcggtcgccg
    10321 actggaacgg gtagaccatg cgaatcgtgc tgcgcccggc gatcatgccg agcagggcgg
    10381 ccagggcaga cggccggttg cgcgccacga acgcgactct tgcgcccggg ccggcaccgc
    10441 tggcgacgat cagctcgccg acgcgctcgc ccacccggcg catcgcgccc cagctcgtcc
    10501 attgcttctg gaactcgatc gccggctgtt gcgcggggcg tgcgagcgcc gctgcgctca
    10561 gttgttcgag gtcgtccatg cgcgctttac attccctgcc gttgcatcga ccatactcag
    10621 caacgttgta tagtgcttcg aggaaaaatt aaacagctgt gaacacgaac actgttacgg
    10681 tcatcatcgg ggcagggcag gcgggctgtg acctggccac ggcattgcgc cagcaaggtt
    10741 atgcggggcg cattatcttg ctgggcgagg agccggtgcc gccttatcgt cgtccgccct
    10801 tgtcgaagac ctatctctcg ggagacatcg gcgaggaagc gctttacatc aagccccgcg
    10861 cggcctatgc gaagcaggat atcgatctgc gcaccggcgt gcgcgtgacc gccatcgatc
    10921 gccaggcgca taccgtcagc ctggacaacg gcgagcgcat cgcctacgac aagctggcga
    10981 tcacgaccgg tgggcgtccg cggcaactcc cgctggcggg cgccgacaag ccgaacgtgc
    11041 attacgtcag gaccctcgag gacatcaacc gcctgcgcga gcagttcgag ccggggcggc
    11101 gactggtcat catcggtggc gggtacatcg gcctggaagc cgccgccgtg ggcatcaaga
    11161 aggagctcgc ggtcacgctg gtcgaggcat tgccgcgcct gctggcacgc gtgaccggcc
    11221 cggagctctc gcagtattac gccggcgtgc accgccgtca cggcgtggac atacgcctcg
    11281 gcgcgggcgt cgaggcgctg gagggcgggg agcaggtgga attcgtcgtg ctgcaggacg
    11341 gcacccgtct gcccgcggac gtggtgatcg tcgggatcgg cctggtcccg aacacggaac
    11401 tggccgaaca ggcgggcctg gaagtagaca acggcatcat cgtcgacctg tacgcccaga
    11461 ccagcgatcc cgatatcgtc gctgccggcg attgcacgaa ccacgacaac ggcttcctcg
    11521 ggcgcaggct tcgcctggaa tccgtgccca atgcgagtga gcagggccgc gtggctgccg
    11581 catcgatttg cgggcagcgg gtggcccatg ccggtgtgcc ctggttctgg tcggaccagt
    11641 acgacctcaa gctgcagatg gtgggcctgt cgcagggcca cgatcgcgtg gtcatccgcg
    11701 gttcgatgga ggcggactcc tttgccgctt tctacctgca agacggcgtg gtcatcgctg
    11761 tcgatgcggt gaatcgcccg caggacttca tggtgggcaa gaagatggtc gccgccaggc
    11821 tgaaggcgga ccccgcaacg ctcgccgacg aatccgtcgt gctcaagtcg ctcgtcgcct
    11881 gacggggaaa agcggccagc ggcgtgaccc cgacagcgta tgcgcgcgtg ctgctcggct
    11941 ccctggggcg cgaagagctt cagccggcag aggacaagcg cggcgccgcc ctgctgtggg
    12001 cacaatccgg tgcgatgtgg ttgagcggac atgccgacgg tccggcgcgc agctgcccgg
    12061 caccgctcgc cgcctgcgcc cagggcgcgt ggctggcgtt gtcggcgctg cgtccgcagc
    12121 actgcgatcc gcgtttcgat gcctaccgtc tgctcggcga gcgcgccgcg atcgcgggcc
    12181 tcggtcgacg cgggcgcgtg tctgccggcg gggcctgcca cctgctcgat accgccgacg
    12241 gcgcgctggc cctgaacctc gcccgcgacg acgaccacga tctgctgcct gcgtggctgg
    12301 agcagccggt ggcgggcatg ggagagcttg ccgaaatcct gcgcgctcgc ccgacggtgg
    12361 cgctggttca gcgcgcgcgg ctgctcggtc tcgcagccgc tgcgctggtt gcgccgcggc
    12421 cgcgccggac gtggtttcac gccaggcgcc acgccccggc gcggcgcgac ggccgcgctg
    12481 caccgctggt catcgatctg agctcgctgt gggcggggcc gctgtgcgcg cagctcctgg
    12541 ccggctgcga cgcgcgtgtc atcaaggtcg agagcacggc ccgtcccgac ggtgcgcgcc
    12601 gcgggccggc gtggttctac gatctgctga atgccggcaa ggaaagcgtc gcactcgatt
    12661 tgcgcgagcc ggccgatcgc gggcgattgc acgccctgct cgcacaggcc gatatcgtca
    12721 tcgaatcggc gcgcccgcgg gcactggagc agatggggat acacgccgcg caactcctcg
    12781 ccgcgcgccc cgggatggtg tggctcggaa tcaccggtta cggtcgccgc gcgcctatgc
    12841 gcgactggat tgcctacggc gatgacgccg gtgtggcggc ggggctcgcc tggctcatgg
    12901 ggggcgcgcg gggcgacccg gttttctgtg ccgatgccat cgccgatcca ttgaccggcc
    12961 tgcacgccgc agcgctggcg ctcgcggcct ggctggacgg cggcgggcag ttgctggata
    13021 ttgcgttgca cgacgtcgcg gcgttctgcg ccggtgccgg ttgcgagtcg gacccgggcg
    13081 cggtaccgct ggtggaggct gcattgccgc tcgcgcgggt ggcgtgcgag caagccgccg
    13141 aaccgggcgt cgacacggcg cgcgtgttgc gcgaattcac gtgagggttt gccgcattgt
    13201 ccgtcactga agcggggagt cgcgacgacg catcgctgct gatccggcgc gccctgctgc
    13261 cacagcgggg attgttcgac ctgcgcatcg gcggcgagcg gatcgttgcg ctgcagcggg
    13321 aactggagcc atgcgccggc gagcgcatca tcgatgcgcg cggcaacgtg ctgctgccgg
    13381 gactccacga tcaccacctg cacctgttcg cgaccgcggc ggcacgcgcc tcgctgctgt
    13441 gtgggccgcc ggccgcgcac gaagagcaat cgctgcgcca ggcgcttgcc cggcattgcg
    13501 cgcagggcga gggctgggtt cgcggcatcg gttttcacga gagtgcctgc gcgcagctcg
    13561 accggcactg gctcgacgcc gtatgcgccg agcggcccgt gcgcatccag catcgcagcg
    13621 gcatgctttg ggtgctcaat tcctgcgcgc tcggccacct gcgcgtggcg ccggacgaat
    13681 gcctgcccgc gggcgccgag cggactgcgg acggcgaatt gaccgggcgc tttttcgatc
    13741 tcgatgcgtg gctcggctcg cggctgccca gggcatggcc gtcgctgcgc gacgtgtcgg
    13801 ccgaactggc gcgccacggc ataaccgcgg tgaccgatgc cggcgcccgc aacgggcaag
    13861 cggcctggga agccctgtgt ggcgccagcg cgcgcgggga gctcgtgcag cgcgtgctgg
    13921 tgatgggcaa cgaggaactg gcggtgtcgg caggcgcatc gccggggcgt atcgaggtcg
    13981 ggccgctgaa aatctacctg cgcgagatcg ggttgccgga gttcgaggca ctggtgttgc
    14041 gcgtgcaggc ggcgcacgcg cagggacgcg ccgtcgcatt tcactgcgtg acgcgggtcg
    14101 aactgacatt tgcgctcgcc gccctggcgc aggcggggac gctgcccggt gaccgcatgg
    14161 aacatgcggc gatcgccgac gagcacgcac tgcaggcact ggcagcactc ggggtgacgg
    14221 tggtcacgca gccgcaattc atcgcggagc gcggagatca atacctgcgc gacgtggagg
    14281 cggtggacat cccgttgctg taccgcggtg ccgggttcct gcgcggcggt gtggcgctcg
    14341 ccgccggcag cgatgcgccg tacgggtcga cggatccgtg ggccgccatg cgcgccgcca
    14401 tcgggcgtcg cacgcgcgca ggcgtgcaga tgaatgccgg cgagtgcctg gcaccggcgc
    14461 aggccatcgc gctgttcgcc ggggatctgc accggcccgc gagcggcttg cgtgaactcg
    14521 ccgtcggcca gcccgcggac ttgtgcctgc tcgatgtgtc ctgggaggat ctgtgcgccg
    14581 atcccgatgc gcgtcacgtg gcgctcacgg tctgcgccgg gcggatcgtg tacgcggcgc
    14641 cacgccatgc atagggcaat catcgcgagc gtcgacgagc tgccgcagga tccgcagtgg
    14701 ctgggatccc ggccctgtcc cgtgattgcc tacggtgcgg gtgcagcgag cgcgtcagcg
    14761 gatgtgctgg tgcccgacct cgccgcggca cgtgccatcg agaagcgggt gctggcctgg
    14821 cccgtcgcag cgatgacact ggtgcaggtg ctgcgcagca ccgcactgct gcccgccgcg
    14881 caggcgctcg atgttgaatc gatggccttt gcgaccctgc agggcggcga ggaattcgcc
    14941 gcctggcagc gcagcgccag gcgcggtccg ccgccgctgc cggtgcaagc gggcgacccg
    15001 gtgttgctga gccgcgaggg tgccgtgatc gaggccgtgc tcaaccgtgc cggcacgcgc
    15061 aacgccatca gtgtcgaaat gcgcgacgcc tgggtcgagg ccctgcagct gctcgaggcg
    15121 gacgagtcgg tcgaggaact gcggttcagg gcgctgggcg cctgcttcag cgtcggcggc
    15181 gaactgcacg aattcgggct cgctcccgat ccggccaccg cacactgggt gcgcacggtg
    15241 cacagccccg cgcgcctgct ggccaggctg ggcacacggg tgagcttttt cgtgcacggg
    15301 gcatgcatcg gttcgggcat cgaactgccg gcattcgccg cgcggcttgt cgcgcacccc
    15361 gaaaccttct tccagctgcc ggaactccag atggggctga ttcccggcgc gggcggcacg
    15421 gtgagcattg cgcggcgcat cgggcgccag cgcacggcgt ggatggtcct gacgggccgg
    15481 cggatcagcg cgcgcacggc cctggagtgg gggctggtcg atgcgcttgt cgacgtgagc
    15541 tcagcgccac cgtgatgcgc gcatgcgccg ttgcaggcaa attccttttc ccggacagtc
    15601 attggcgggc gctttcgcgc cactatgatg gcatcgatgc cgattccgcg ttgaattccc
    15661 ggggcacgca cgacaggatc cgatccacat gaagacgaca ctgattcgca acgcaaccat
    15721 ctgggacggg agcggtgccc gcgcctttgc cggcgacctg ctgatcgagg gcaaccgcat
    15781 ccgctccgtc acgcccggcg atgcggaggc cggggcgcgt gccgacgtgc agatcgacgc
    15841 cgccggcagg ttcctgatgc ccggcatgac ggagggccat gcgcacctgt ccttcgatgg
    15901 cgtcacgtgc accgagaacc tgatcacccc gccgcccgag gagcacacgc tgctgacggc
    15961 gcgcgtggcg aagaccctgc tcgatcacgg tttcaccagt gcctacgggg catcggccgc
    16021 gaagctgcgt ctcgatgtcg cggtgcgcaa tgaaatcgac gccgggcgcc tgccggggcc
    16081 gcgtgtgcgt gccggctccc tcgagatcac ggtgaccggg gggctcgggg acgagagcaa
    16141 gctgcacaat ccgcgcaacg gtccgtcgat cgtcgtcgac ggcgccgagg aaatgcgcaa
    16201 ggccgtgcgc ctgtgctgcc gcgagggctg cgacaacatc aagctcgatg tgtccggcga
    16261 cccgttctat cccgagatac cctcgcacgc cacgccgatg tcgctggagg aggtccgcat
    16321 ggcggtggca acggcgcaca gcttcggcaa gaaggtcaat gcccacgcgc gctcgatcga
    16381 gtcggtgcgc cactgcctgg tcgccggggt cgacgtgatc taccacgccg agtacagcga
    16441 cgaggaaacg ctcgacatgc tcgaggcggc gaaggatcgc atcttcgtcg cgcccacgat
    16501 cagcctgttc cataccatga tgcacgaggc ggaaccctac ctgccgcgtg cggccgccga
    16561 ggcaatgggg atcccggctc tgatcgagga gtcggtgaag acgcataccg cgctgcgcaa
    16621 gcgcggcatc cgtcacctga tcggcggcga ctacggcttt gcctggagca tgcagggcac
    16681 caatgcccgc gacctgaagt tcttcatgga ctactacggc tactcggcgg ccgacgcgct
    16741 ggtctgcgcc acgcgcaatg gcggcctcgc gatgcgcgcc cagggcgatc tcggcaccct
    16801 gcgcccgggc atgcttgccg acatgctgct ggtggacggt gatccactga cggacgtgac
    16861 gatcctgacg gacaggcgcc gcctggcgat ggtcatgaag gacggtgaaa tcagcaccct
    16921 ccagcccgcg ctgcacgcga cgggctgaga ggggcgtcgc gcgccgggat caggagccgg
    16981 gcgtggccgc cgccggcgcg aaccagatcg gcgaggacca cgcgcgttcc tgtatcgcgg
    17041 cgggctcctg cgccgccgtg ccgagtttca ccgcgtcgta ggtcgaccag cgcggcgtcg
    17101 ggatttccag cacgcgcacg tagtagaacg cccgcgcctg gggctggaac tccgggtcct
    17161 gccatacggc ggagagctcc gccgcgccga tggtgttgcg atacgttgcc gtcttcacgt
    17221 ccacggtcga gcccaccgcg ggcagcttgc cgctcgcggc atcgatggca cgttcgccgg
    17281 cccaggcgac gtcgaagatc ttctcccgcg ccgtgccatc gacatccagc cagcccttga
    17341 cgatctgcac ccggtcgaga ttcgcgccgt cgggatcctt cgttgccagc accgcgaagc
    17401 ggggtgcgcc gcgcgcggcg ggagcgggca gctgcgagcc catcggcacg ccggaggcat
    17461 aggcctgcgc gacgaaatcc ggcgcctgca cgagcgccgg cgcgaaatcc cagccgccga
    17521 aaaagcgcag gctgatgcgt gtgccggagg tcgcgaaggt ctccttgcgc ctgagcgcgt
    17581 cgtagatcgc ctcgcgcgtg ttggcatcgg cccacacggc ggccagccct gccgcgctgt
    17641 aggcggtttc gcgcccggcg gcccgggcct cggcgctgcg cgcgatacgt cccgcgcgca
    17701 tcgcggccgt gccgtcgagc tgcgtcagct tgccgtggta gttgtcctcc tcgatcggcg
    17761 gcgcggagtt gtgcgtgtcg ctggagccga tcatgccgaa gtcgtagggg ttcacgccga
    17821 ggctggcgcc gagctcgagc ccggtcttca gcgcatcgcg cgcgtagctg ccctcgggtt
    17881 tggaggcgcg cgggatcttc gcgcccgaca tgcgcgtggt ccacatctcg tagttggcga
    17941 actcgtcctc ggtcgagagc agcggatgcg cctccgactg gcccttgatc tgcacgatct
    18001 cggcgacagg ctcgttgcgg ctgcgctggc gcgcccacgc cgcatcgacc ggtctgcccg
    18061 cggaatccgt gcgcgcccac atcatgccgt cgctggcatt cgaattgtgc gggatcgcca
    18121 tgccctcgat accctgcgcg cgttgctcgt cgagaaagcg ccacaggtcc tcgggcttgt
    18181 cggaatcgat gcgcgagaaa ggcttggggg gtaccgcgga gccgcggaat atcacgttgc
    18241 ggtgcaggtt gatctcatcg ggcgtgctgg tccactcgta gccgatcagc gtggtgaagc
    18301 gccccggctc gttgtggcgc tccgcggcgg cgatcacttc gcgccaggcg gccatgttca
    18361 cctccggcag gtcgagcagt tcgtcgaggc tgccgtcgtg cgaggcgtcg tcggagatct
    18421 gcatcagcgc cttgaaagca tccgccggat cggggctggc gaagagcttc gccagcggat
    18481 gctgcgacaa agggctcgcg gggttgagga attcgcgcat cgagccgagg tactcggagt
    18541 gttcagtgac ggcggcgaaa tcgagcgggc gcttcagctg cacggtgaag ccggcggcgt
    18601 gcgcgattgc ctcgcccctg gcgtagcggt aggcgtcatc gggcagcgtg cgcgtgccgg
    18661 tgctgaaggc atcggccgac agcgcggtgt ggatgtgcag atcgccataa agcacctgcc
    18721 gtgccgcggg agcggctgtg ttcgccgcag cggcagcgcc ggcctcggcg agcagcaggg
    18781 ttgcacaact caacagcgcc agcggcagtc gtcgcatcgt cacgtcctcg tcgtggttcc
    18841 agatgccgtc atgttaaccg cgagtccggc gtcctcgaca gacccggtgc cgccacggcg
    18901 ttgggcggcc cgtgtgtacg gggctatgat gccgactcgc ctgcccgcaa cagaaggaac
    18961 ccgcgatgaa cgacccggct aacctggagc tcggcctgat cggcctgggc acgatgggca
    19021 tgagcctgct gctgaacatg gccgatcacg gccacgcggt cgcgggctac caccgcgacg
//
LOCUS       DAORUQ010000036        19068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2509_length_19068_cov_7.256877, whole genome shotgun sequence.
ACCESSION   DAORUQ010000036 DAORUQ010000000
VERSION     DAORUQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_19068_cov_7.256877"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1291
                     /locus_tag="PLW13_05140"
     CDS             389..1291
                     /locus_tag="PLW13_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY69788.1"
                     /translation="MQGALQIEDVIELPHVRMERVTQDASGPFFRAHRVNEPSLIYIP
                     PPHRESARGSYGEPGSPGSLMSFGAAVFLPGAGSVHIQSPGFPKRTMLVLRFLDDEMK
                     NLVNQLNSEDHGLMMKFADLRHTRIIEIIDRLSQEMNKDAVGRDLILKGLGLVLVGEM
                     ARYLASVSNDPPLERGTLADWQMRRISERLTDESKAPPGVPELAAICGLSRRHLMRAF
                     KATTGMTVMEWVEHGTFDRAMRLLGGADSIKSISARLGYACPGSFASAFARKFGVPPR
                     QWRRRNGRGSGQSPPASRSLIPSR"
     gene            complement(1249..2466)
                     /gene="serB"
                     /locus_tag="PLW13_05145"
     CDS             complement(1249..2466)
                     /gene="serB"
                     /locus_tag="PLW13_05145"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HQY69789.1"
                     /translation="MNRIVLINISGEDRPGITSMLTGILAAHGINVLDIGQAVIHSHL
                     ALGLLVEIPEERETSAALKELLFHAHEAGLQLRYTAVSAASYREWVAGQGKPRHIVTL
                     LSRRIRGEEIARLTEIVARHGLNIDKINRLSGRVSLDAAAESDRACVEFSVRGDLADA
                     AAVRRDFLELASELDVDIAYQEDNMFRRTRRLVAFDMDSTLIEAEVIDELAKRAGVGE
                     QVAAITERAMRGEIDFSESFRQRVALLAGLDESVLEEVAAQLRLSEGAEKLIATLRRI
                     GFRTAIISGGFGFFGRRLQQRLGIDYVFANELDIEDGRVTGRVVGTIVDGRRKAELLR
                     EIAARESISLEQVIAVGDGANDLPMLSIAGLGIAFRAKPIVKESARHAISTLGLDGIL
                     YLLGISDREAGGL"
     gene            complement(2472..4727)
                     /gene="parC"
                     /locus_tag="PLW13_05150"
     CDS             complement(2472..4727)
                     /gene="parC"
                     /locus_tag="PLW13_05150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HQY69790.1"
                     /translation="MTETQAPPSGDIERIRLRDYTEKAYLDYSMYVILDRALPHIGDG
                     LKPVQRRIIYAMHELGLRASAKYKKSARTVGDVIGKFHPHGDSAAYEAMVLMAQSFSY
                     RYPLIDGQGNWGAPDDPKSFAAMRYTESRLAAIADVLLSEVGLGTVDWMPNFDGTIDE
                     PAVLPARAPHILLNGTTGIAVGMATDIPPHNLRELTAACMQLLDRPGTTAAELTEWVR
                     GPDYPTEAEIITPAHELRALYTTGRGSLRARAVWIREQGDLVITALPHQVSGARVLEQ
                     IAAQMQAKKLPMVADLRDESDHENPTRLVIVLRSNRVDADGLMSHLFASTDLERSYRV
                     NMNVIGLDGRPGVRDLAAMLREWLEFRRATVRRRLQYRLEQVLRRLHILDGLLVAFLN
                     IDEVIAIIRHEDKPKPALMARFGLSDEQAEAILELKLRHLAKLEEMKIRGEQAELAEE
                     RDTIEKTLGSEARLTTLIKKELQAVADEYGDARRSPLVSREEARAYSETELLSSEPVT
                     VVLSKMGWIRAAKGLDIDGEKLSYKAGDAFKCAVQGRSNQSVVLLDSTGRTYSLPTHS
                     LPSARGQGEPLTGRINPPSGATFAGALMGEDAHLYLLASDAGYGFVAKLEDMQSKNRA
                     GKAVLTLPEGAEVLAPVRVHDVAKDVLVAVSNEGRMLVFPVAELPMLARGKGNKIIGI
                     SGTLLRTREEYLLGVVVMAPNATVVVHSGKRYIKLKGSDLDAYRAERGRRGNKLPRGF
                     QKVDRVEIATD"
     gene            complement(4801..5400)
                     /locus_tag="PLW13_05155"
     CDS             complement(4801..5400)
                     /locus_tag="PLW13_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HQY69791.1"
                     /translation="MPRAWRLAPVLALAAVLAACGSTPRTEYYLLSAEAPPAAARAQP
                     SIGIAELKVAEYLQRPEMVTMESANRLSLRDYHRWAEPLADGVQRTVALNLGALLETD
                     AVRVRPWPREWSPQWLLRLNIARLDVRGDTVDLVATWSLTSASETRERSSRLTRSRSG
                     TSADSVAADISAVLLELSGQIAAEVRAAAGADDAPAQTQ"
     gene            complement(5400..6380)
                     /locus_tag="PLW13_05160"
     CDS             complement(5400..6380)
                     /locus_tag="PLW13_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQY69792.1"
                     /translation="MSGKPNPTAIGAFVVGAIALLLGATLFFGAKLFSGSSGRFGATA
                     IFTGSVKGLDVGAPVTLRGVKIGEVTDIHVSFDQRTAEFVIPVTMSLSREDLGIAKDL
                     ATRGNLQVLIDRGLRAQLKTQSLLTGLLYVDLVFKPESPPRYVDFDTEEPQLPTAPTG
                     LEAIMERMSEVDLQSIIQNADQTLRAVSSLLANPETQRIPANINAALADVRSLVANAD
                     RQVALIGSRLDTLAGSADGTLGVLRVQVKDTGTRLDASLQALDQTLASLRVTSDEAGY
                     ALSEQSPVFHELTRTAAELGRAGRALQALADALAREPESLLRGRSDRSDE"
     gene            complement(6377..7141)
                     /locus_tag="PLW13_05165"
     CDS             complement(6377..7141)
                     /locus_tag="PLW13_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY69793.1"
                     /translation="MDAAQPHIEVRGLTMAYGSRVIQKDLDFTVNRGDIFVIMGGSGC
                     GKSTLLKHMIGLYEPAAGEILYGGASFQRADDGERDRMRRRWGITYQGGGLFSAMTLA
                     ENVALPLQQYTPLDAATIGELVSYKLALVGLAGFEEYYPSEISGGMQKRAGLARAIAL
                     DPEILFFDEPSAGLDPISSRLLDELILHMRDALGATVVMVTHELASIFAIGNNSVFLD
                     AETRTMLDYGDPQRLLTGSDHELVRRFLSRSAGESA"
     gene            complement(7144..8289)
                     /locus_tag="PLW13_05170"
     CDS             complement(7144..8289)
                     /locus_tag="PLW13_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69794.1"
                     /translation="MSAAPAADPNPARCAIEARAGGTTLVLSGDWTVARGAPRAADVL
                     PQLRAQPARELGFDCNALGSWDSLLVMLLLGIEEECTRAGMRFDSANLPEGALRLMAL
                     ARAVRPEQHARPARAPWWRRLLAGEPLLRAWGELLGALAFLGELLIALGKLCLGRANT
                     RARDFLWFVQQAGPAAIGIVTLISVLVGMILAYLGSVQLRQFGAQIYVADLVGIGMVR
                     EMAALMTGVIMAGRTGAAYAAQLGTMQTREEIDAIRTLGMSPMEFLVLPRLLALVLVM
                     PLLTLYSGVLGMCGGALVATGMDVSWTQYLHETRLAVNLTHISTGLAKSVVFGLLIAL
                     AGCRAGIQCGRSSEAVGKATTRAVVTAIVALIVADAAFNIVYQRLGI"
     gene            complement(8286..10181)
                     /gene="parE"
                     /locus_tag="PLW13_05175"
     CDS             complement(8286..10181)
                     /gene="parE"
                     /locus_tag="PLW13_05175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQY69795.1"
                     /translation="MAVSAQYNAEAIEVLTGLDPVRKRPGMYTDTTRPNHLAQEVIDN
                     GVDEALAGFARAIDVVLHADGSLSVADDGRGMPVDIHPEQGKPGVEVILCTLHAGGKF
                     SEGSYQFSGGLHGVGVSVVNALSLELEVTVRRDGNVYRMRFENGNKASELEIIDSVAR
                     RSTGTTVRFRPDPRYFDTVKFSVSRLTHLLRAKAVLCPGLNITFTDEATGQREAWYYE
                     NGLPDYIRGATAEYPTLPAEPFVGSFSGKTEAVDWAVQWLPEGGELITESYVNLIPTA
                     QGGTHVNGLRTGLLEALREYCEFRSLLPRGIKLSPEDLWDRCAYVLSAKLREPQFSGQ
                     TKERLSSREAAAFIAGVARDAFSLWLGQHTEEGDKLADMAVSAAAARLRKSRQVERKK
                     ITSGPALPGKLADCTAQDLDRTELFLVEGDSAGGSAKQARDRVFQAIMPLKGKILNSW
                     ELESSEILASEEIHNIAVALGVDPGSADLGGLRYGKVCILADADSDGLHIATLLCALF
                     VRHFRPLVQARRVFVAMPPLYRIDIGKDVYYALDESEKEGVLNLIEAEKKKGKVNVQR
                     FKGLGEMNPLQLRETVMTPDTRRLVELTLSEEDQADQLLDMLLAKKRATDRRDWLERS
                     GNLAQRI"
     gene            complement(10237..11061)
                     /gene="cpdA"
                     /locus_tag="PLW13_05180"
     CDS             complement(10237..11061)
                     /gene="cpdA"
                     /locus_tag="PLW13_05180"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HQY69796.1"
                     /translation="MTDTRVDLALAGSCVRVVQVTDTHLEERRGGTLLGMDTDASLAH
                     VLDLVRDGPLRPDLLLATGDLANHGAEAAYVRVRESFDALGLPWFWLPGNHDAGPLMH
                     GVIGRGRPMIRSICAGSWQIVMLDSTVAGQVGGSLGAAELALLDRLLGAEPARHALVC
                     LHHQPVAIGCAWLDEQMVADADALFAVLARHPQARALLWGHVHQEFSAERAGLRLLGS
                     PSSCIQFAPCSEGFRLDEQAPGLRWLELHPDGRLETRVERVSGVELGYDRHSAGYL"
     gene            complement(11074..11568)
                     /locus_tag="PLW13_05185"
     CDS             complement(11074..11568)
                     /locus_tag="PLW13_05185"
                     /inference="COORDINATES: protein motif:HMM:NF018552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HQY69797.1"
                     /translation="MSFPRHAGQAAQGRDAAVAPCSPLQIHHAECEVNFLRLEKLLPG
                     FVAGQARHIGTRLPRGGMDVLHLHVVQRCPYTAEVVLRQQQPVWGARGAEFTVRAYLD
                     ARMAEVVGCARVRRLLARYDYPNRDGLARDEKWQINRLLGEWLAHCLAEGHALHSAAL
                     AADG"
     gene            11741..13180
                     /locus_tag="PLW13_05190"
     CDS             11741..13180
                     /locus_tag="PLW13_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQY69798.1"
                     /translation="MLHSRLRALITTSLLLLPAALRADTLLDIYELAVQNDPVIRAAE
                     ATYRAGREYEVQGRSQLLPQVGAQAEYGEDDMTQKSTRTIAIGDQTFTSSSNYDVEED
                     QENYYLTLSQKLFDVPAWFSFKQGKELSKQAEAQFAADQQALIVRTVEAYVLVLRAID
                     NLKSSRAAEAAFQRQLEQTQQRFDVGLIAITDVHEARAAFDLAVVERLTDEGNLGVAY
                     EALSVLTGQDHANLWLLSEEFPVVDPQPAAAESWVELAMEGNLGLKVASYAAEAAMQS
                     SQASRAQHLPKLSGQLTLMDTSTDGDNVDNNLRDPAGNRLNPRYPTSVDVEGNSVMLT
                     LSMPLYAGGGVSSQRRQSYENYIAARETHTSIMRDTIQFTRSLHLFVTTDVQRVKARR
                     QAIVSAQSALDATTAGYEVGTRNVVDVLNAQQVLFTAIRDYANTRYDYVSRLLRLRQQ
                     AGLLSPEDVVALNKWLVEPTPATATAPRI"
     gene            complement(13190..13576)
                     /locus_tag="PLW13_05195"
     CDS             complement(13190..13576)
                     /locus_tag="PLW13_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY69799.1"
                     /translation="MPFDTRVLVVDDFESMRRIVGQLLRDMGFRDVTLADDGATALAL
                     LRQDDFGLLLTDWHMPQMDGLDLVRAVRADPRLNAIPILMVSTEATREQIIEAVRAGV
                     NGYVVKPFTPQILRAKIGKLFQSPAR"
     gene            13692..14312
                     /locus_tag="PLW13_05200"
     CDS             13692..14312
                     /locus_tag="PLW13_05200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY69800.1"
                     /translation="MGIKLPLDIDSVKGFLSPVEGAALYRAALECAPLGPCLEIGSYC
                     GKSTVYLGTACQRSGGVLFAVDHHRGSEEHQLGEEYHDPALYDGAAGLMDSFREFRAT
                     MRRAGLDDTVVPVVAPTRVAARAWRTPLSLVFIDGGHSLEAAMADYRGWSGHLVPGGL
                     LAIHDIFPDPADGGQAPYTIYRLALASGLYEELPRVETLGLLRRIA"
     gene            complement(14250..15338)
                     /locus_tag="PLW13_05205"
     CDS             complement(14250..15338)
                     /locus_tag="PLW13_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HQY69801.1"
                     /translation="MSELYLSKGLYPAEFLRPSVDFILATQQADGCIPWFPGGHADPW
                     DHVEAAMGLGIAGEFDAAARAYRWLARTQLPDGSWWASYKDGEVLERGRRETNFVAYI
                     ATGLWHHYLVSGDRELLDELWPVVERAIEFVLSQQTVHGEVCWAVDAEGNTMDDALVT
                     GCSSIHKSLECAINIAHALYRPCPRWVEARWRLGEALRHRPERFDRTWESKARFSMDW
                     FYPVLGGVLGRGQAKARLAARWDTFVKEGMGCRCVSDEPWVTVAESCELTMALLGAGD
                     HARAARLFSWLHQWRDHDGAYWTGYQWVENIMWPEEKPTWTAGAILLAADALTGHTGA
                     SRLLTEVRLSDSPQEAKRLNARQFFVQP"
     gene            complement(15335..16057)
                     /locus_tag="PLW13_05210"
     CDS             complement(15335..16057)
                     /locus_tag="PLW13_05210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY69802.1"
                     /translation="METVNLDYVPIRRGDRVLDLGCGEGRHAIAAWLRAEVHCVGIDL
                     SVQDVATAAARARPFAPERPGTAHCSLGFGVGNALQLPFADHCFDVVICSEVLEHIGP
                     YREVLAEIRRVLKPGGALVASVPRYWPERLCWALSDAYHANEGGHIRIFRAARLNRDI
                     EALGFRRYRRHWAHALHAPFWWLKCLLWERQHESRLVALYHRLLVWDLMERPRLTRWA
                     EALLNPVLGKSVVMYFQKAPVP"
     gene            complement(16038..>16597)
                     /locus_tag="PLW13_05215"
     CDS             complement(16038..>16597)
                     /locus_tag="PLW13_05215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY69803.1"
                     /translation="LQLMATASADQPLKGLAVLLNAFAALLPAHPGLRLLVVGKPRPG
                     GDTEKLIARLDLGAHIDFVSGISTAELVRLYRESTLVVVPSLYEGFGLPASEAMACGA
                     AVVSSDGGALPEVVGDAGVLVPAGDETALAGAIAALLADPARRTELGQRARARMSTLF
                     CWRRAARQMTHYYEQVLAGDGNRQS"
     assembly_gap    16598..16697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16698..17512)
                     /locus_tag="PLW13_05220"
     CDS             complement(<16698..17512)
                     /locus_tag="PLW13_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY69804.1"
                     /translation="MEALNPARLPLDRYPEALSLQDLEPVRPLRICLLGYRSHPYGGG
                     QGVYLRYLSKALLELGHRVDVISGPPYPHLDPRVRLIEMPSMNLYETGLLSLRPRHLR
                     SLSNVIEWLSKLTGGFAEPQAFGRRALRYLREHRDDYDIVHDNQSLSYGMLALQRAGV
                     PLVTTIHHPITSDLRLALAACRWWWPRLLTRRWHTFLGMQKRVARGLRHVVTVSECSR
                     GDIARDFGLPAERLTLVHNGVDTEVFAPDAGVARKPLQLMATASADQPLKGLAV"
     gene            17688..18581
                     /gene="rfbA"
                     /locus_tag="PLW13_05225"
     CDS             17688..18581
                     /gene="rfbA"
                     /locus_tag="PLW13_05225"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQY69805.1"
                     /translation="MQQTNYRGIVLAGGSGTRLHPLTSSVSKQLMPVYDKPMIYYPLA
                     TLMLAGIREILIITTPRDQDAFRELLGDGSQWGLELVYAVQPEPGGLAQAFLIGEQFV
                     GDRPVCLVLGDNIFYGGGLSKKLQSAALETDGATVFAYYVQDPERYGVVSFDAQNRAV
                     DIEEKPRHPKSHYAVTGLYFYDNEVVSIAKSIQPSARGELEITDVNRIYLQRGRLRVE
                     TLTRGAAWLDTGTHNSLLDASNFIRVVEERQGLKICCPEEIAYRMGFITREQLLALAR
                     PLKKSGYGLYLERIASEGSPV"
     gene            18581..>19068
                     /gene="rfbC"
                     /locus_tag="PLW13_05230"
     CDS             18581..>19068
                     /gene="rfbC"
                     /locus_tag="PLW13_05230"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQY69806.1"
                     /translation="MQIIATPIPDVLVLEPRVFGDERGFFFESWNQREFARLGLPAQF
                     VQDNHSKSARGILRGLHYQTQQVQGKLVRVVAGEVFDVAVDMRRGSPTLGRWAGALLS
                     AENRRMMWIPPGFAHGFYVTADSAEFIYKCTDFYAPEHEVSLRWDDPAIGIDWPLIDG
                     KPP"
BASE COUNT         2960 a         6594 c         6472 g         2942 t
ORIGIN      
        1 aaaacagcct cctcgaaata ggaatcgaat gcccggcgtt gacctgcact cggtcgcgct
       61 ttttccagcc aataggtgcg gcgatccgca attgtcgcct gcacgcgggg gtccgcatag
      121 actttcaagg cctccagctc gatgcggcgc tgatccgggg tgtcaatagg attgggcata
      181 gtcgcgtccc tttgttgatt tggggtattt ttctcgcgga cggctctctg gagcaatcgc
      241 gaacagtgcg gcgccagcgc cgttattcgg cgattgcgcc gcgtattccg tcagatatcg
      301 gtcgcgcgac gatttcgcgg cgcgacactg gccaggctga tctacccgcc tcgcctcagc
      361 gaacaatgac aaaatcgagg aatgaattgt gcagggagcg ttgcaaatcg aggacgtcat
      421 cgaactgcca catgtgcgca tggagcgggt tacgcaggac gcgagcgggc cattcttccg
      481 cgcccacaga gtgaacgagc cgtcgctgat ctatattcct ccacctcatc gcgagtccgc
      541 gcgtggctca tatggtgagc cggggtcacc cgggtcgctc atgtcattcg gcgcggctgt
      601 attcctgccg ggcgctggct ccgtgcacat tcaatcgccg ggattcccga agcgcacgat
      661 gcttgtgctg cgcttcctcg acgatgaaat gaagaacctc gtcaaccagc tcaacagcga
      721 agaccacggc ttgatgatga agttcgcgga tttgcggcac accaggatca tcgagatcat
      781 cgatcgtctg tcgcaggaaa tgaacaagga cgcggtgggt cgtgatctca ttctgaaagg
      841 cttgggcctg gtgctggtcg gtgagatggc gcgctatctg gcgagcgttt cgaacgatcc
      901 gccgctggag aggggaacac tcgcagattg gcagatgcgc cgaatttctg agcggctgac
      961 cgacgaatcc aaggcgccgc cgggcgtgcc tgagctcgcc gcaatctgcg gtctgagccg
     1021 tcgccatttg atgcgcgcct tcaaagcgac gaccggcatg acggtgatgg aatgggtgga
     1081 gcacggcacc ttcgatcgcg caatgcgcct tctcggcggt gctgattcga tcaagtccat
     1141 ctcggcccga ctgggttatg cctgccccgg cagtttcgcc agcgcttttg cgcgcaaatt
     1201 cggcgtgccg ccgcggcaat ggcgccgtcg gaacggccgg ggctccggtc agagcccgcc
     1261 ggcctcgcga tcgctgatcc cgagcaggta gaggatcccg tcgaggccga gcgtggaaat
     1321 cgcgtggcgc gccgactcct tcacgatcgg cttggcgcgg aatgcgatgc ccaggcccgc
     1381 gatgctgagc atcggcagat cgttcgcccc gtcacccacc gcgatcacct gctcgagcga
     1441 gatggactcg cgcgcggcga tctcgcgcag cagctcggcc ttgcgcctgc catcgacgat
     1501 ggtgcccacc acgcgcccgg tgacgcgccc gtcctcgatg tcgagctcgt tggcgaacac
     1561 gtagtcgatg cccaggcgct gctgcaggcg ccgcccgaag aagccgaagc cgcccgagat
     1621 gatcgcggtg cgaaaaccga tgcggcgcag cgtcgcgatc agcttctcgg cgccctcgct
     1681 gaggcgcagc tgcgcggcca cctcctccag cacgctctcg tcgagccccg cgagcagcgc
     1741 cacgcgctgg cggaagctct cgctgaagtc gatctcgccg cgcatcgcgc gctcggtgat
     1801 cgccgcgacc tgctcgccga cgccggcgcg cttggcgagc tcgtcgatca cctcggcctc
     1861 gatcagcgtc gagtccatgt cgaaggcgac caggcgccgc gtgcggcgga acatgttgtc
     1921 ttcctggtag gcgatgtcca cgtcgagctc gctggcgagc tcgagaaagt cgcgacgcac
     1981 cgccgcggca tcggcaaggt cgccgcgcac cgagaattcc acgcaggcgc ggtcgctctc
     2041 cgccgcggca tcgagcgaga cgcgccccga caggcggttg atcttgtcga tgttgaggcc
     2101 gtggcgcgcc acgatctcgg tgagccgcgc gatttcctcg ccacggatgc gccgcgagag
     2161 cagcgtcacg atatggcgcg gcttgccctg gcccgccacc cactcgcgat agctcgcggc
     2221 gctgacggcg gtatagcgca gctgcagccc ggcttcgtgg gcgtggaaca gcaactcctt
     2281 cagcgcggcg gaggtctcgc gctcctcggg aatctcgacc agcaggccga gcgcgaggtg
     2341 gctgtggatc acggcctggc cgatatcgag cacgttgatg ccgtgcgccg cgaggatccc
     2401 ggtcagcatc gaggtgatgc cggggcggtc ctcgccggag atgttgatca gtacgatcct
     2461 gttcatgcgc ctcagtccgt cgcgatctcg acgcggtcga ccttctggaa gccgcgcggc
     2521 agcttgttgc cccgccgccc gcgctcggcg cggtaggcat cgaggtccga tcccttgagc
     2581 ttgatgtagc gcttgccgct gtgcaccacc acggtggcgt tgggcgccat caccacgacg
     2641 ccgagcaggt attcctcgcg ggtgcgcagc agcgtgcccg agatgccgat gatcttgttg
     2701 cccttgccgc gcgcgagcat cggcagctcc gccaccggga acaccagcat gcggccctcg
     2761 ttgctgaccg ccaccagcac gtccttcgcg acatcgtgca cgcgcaccgg cgccagcacc
     2821 tcggcgccct cgggcagcgt gagcacggcc ttgccggcgc ggttcttcga ctgcatgtcc
     2881 tcgagcttcg ccacgaagcc gtaaccggca tcgctagcga gcaggtacag gtgcgcatcc
     2941 tcgcccatca gcgcgccggc gaatgtcgcc cccgagggcg ggttgatgcg cccggtgagc
     3001 ggctcgccct gcccgcgcgc cgatggcagc gaatgcgtcg gcagcgagta ggtgcgcccg
     3061 gtggagtcca gcagcaccac cgactggttc gagcgcccct gcaccgcgca cttgaaggcg
     3121 tcgcccgcct tgtagctcag cttctcgccg tcgatgtcga gacccttggc ggcgcggatc
     3181 cagcccatct tcgacagcac cacggtgacc ggctcggaac tgagcagttc ggtttcgctg
     3241 taggcgcgcg cttcctcgcg gctcaccagc ggcgagcggc gcgcgtcgcc gtattcgtcg
     3301 gccaccgcct gcagttcctt cttgatcagc gtggtcaggc gcgcctccga gcccagcgtc
     3361 ttctcgatcg tgtcgcgctc ctcggccagt tcggcctgtt cgccgcggat cttcatttcc
     3421 tccagcttcg cgaggtggcg cagcttcagc tcgaggatgg cttcggcctg ttcgtcggag
     3481 agcccgaagc gcgccatcag cgcgggcttg ggtttgtcct cgtggcggat gatcgcgatc
     3541 acctcgtcga tgttgaggaa ggcgaccagc aggccgtcga ggatgtgcag gcggcgcagc
     3601 acctgctcga ggcggtactg caggcgccgg cgcaccgtcg cgcggcggaa ttcgagccat
     3661 tcgcgcagca tcgcggcgag gtcgcgcacg ccgggacgcc cgtcgaggcc gatcacgttc
     3721 atgttcacgc ggtagctgcg ctcgagatcg gtgctcgcga acaggtgtga catcagcccg
     3781 tcggcatcca cgcggttcga gcgcagcacg atcacgagcc gtgtcgggtt ctcgtggtcc
     3841 gattcgtcgc gcaggtccgc gaccatcggc agcttcttcg cctgcatctg cgcggcgatc
     3901 tgctccagca cgcgggcccc cgacacctgg tgcggcaacg cggtgatcac gagatcaccc
     3961 tgctcgcgga tccacaccgc gcgcgcgcgc aggctgccgc ggcccgtagt gtagagcgcg
     4021 cgcaactcgt gcgcgggcgt gatgatctcg gcctcggtgg gataatccgg gccgcgcacc
     4081 cactcggtga gctcggcggc cgtggtcccg gggcggtcga gcagctgcat gcacgcggcc
     4141 gtgagctcgc gcaggttgtg cggcgggatg tcggtggcca tgcccaccgc gatgccggtg
     4201 gtgccgttga gcaggatgtg cggcgcgcgt gccggcagca ccgcgggctc gtcgatggta
     4261 ccgtcgaagt tcggcatcca gtcgaccgtg cccagcccca cctcggacag cagaacgtcg
     4321 gcaatggccg cgaggcgcga ctcggtgtag cgcatcgcgg cgaaggactt gggatcatcg
     4381 ggcgcgcccc agttgccctg gccgtcgatc agcgggtacc ggtaggaaaa ggactgcgcc
     4441 atcagcacca tggcctcgta ggccgcgctg tcgccgtgcg ggtggaactt gccgatcacg
     4501 tcgcccacgg tgcgggcgga cttcttgtac ttggcgctcg cgcgcagccc gagttcgtgc
     4561 atcgcgtaga tgatgcgccg ctgcaccggc ttgaggccgt cgccgatgtg cggcagcgcg
     4621 cggtcgagga tcacgtacat cgaatagtcg aggtacgcct tctcggtgta gtcgcgcaga
     4681 cgaatgcgct cgatgtcgcc gctgggcggc gcctgggtct cggtcatgcc tgtctccggc
     4741 cgtaatgcta gcccgggctg gggatcgcgg gggcccgcgc cgcgcgcggg gcggcatatg
     4801 ctattgggtt tgcgccggcg cgtcatccgc gccggcggcg gcgcgcacct cggcggcgat
     4861 ctgcccgctc agttccagca gcacggcgct gatgtcggcc gccaccgagt cggcgctcgt
     4921 gcccgagcgc gagcgcgtga gccggctgct gcgctcgcgc gtctcgctgg cgctggtcag
     4981 cgaccaggtc gcgaccagat ccaccgtatc gccgcgtaca tcgagacgcg cgatattcag
     5041 cctgagcagc cactggggcg accattcgcg cggccacggc cgcacgcgca cggcgtcggt
     5101 ttcgagcagc gcgcccaggt tcagcgccac ggtgcgctgc acgccgtcgg ccagcggctc
     5161 ggcccagcgg tggtagtcgc gcaggctcag gcggttggcg ctctccatgg tcaccatctc
     5221 ggggcgctgc aggtattccg ccaccttcag ctcggcgatg ccgatcgagg gctgcgcgcg
     5281 cgccgcggcc ggcggcgcct cggcgctcag caggtaatat tccgtgcgcg gggtcgagcc
     5341 gcaggccgcc agcacggcgg ccagggccag caccggcgcg aggcgccacg ctctgggcat
     5401 cattcgtcac tcctgtcgct gcgcccgcgc agcagtgatt ccggctcgcg cgccagcgcg
     5461 tcggccagcg cctgcagcgc gcggccggcg cgaccgagct cggcggcggt gcgcgtcagt
     5521 tcgtggaata ccggcgactg ctccgagagc gcgtagccgg cctcgtccga ggtgacgcgc
     5581 aggctggcga gcgtctggtc cagcgcctgc aggctggcat ccagccgcgt gccggtgtcc
     5641 ttgacctgca cgcgcagcac gcccagcgtg ccgtcggcgg agccggcgag cgtatcgagg
     5701 cgcgacccga tcagcgcgac ctggcggtcg gcgttggcga ccagcgagcg cacgtcggcg
     5761 agcgcggcgt tgatgttcgc gggaatgcgc tgcgtctcgg ggttggcgag cagcgaggag
     5821 accgcgcgca gcgtctggtc cgcgttctgg atgatcgatt gcaggtcgac ctcgctcatg
     5881 cgctccatga tcgcctcgag cccggtgggc gcggtcggca gctgcggctc ctcggtgtcg
     5941 aaatcgacat agcgcggcgg cgattccggc ttgaacacca ggtccacgta gagcagcccg
     6001 gtcagcaggc tctgcgtctt gagctgcgcg cgcaagccgc ggtcgatcag cacctgcagg
     6061 ttgccgcgcg tcgcaaggtc cttcgcgata ccgaggtcct cgcggctgag cgacatcgtc
     6121 accgggatca cgaactcggc cgtgcgctgg tcgaagctca cgtggatgtc ggtcacctcg
     6181 ccgatcttca cgccgcgcaa cgtcaccggc gcgcccacgt cgaggccctt gaccgagccg
     6241 gtgaagatcg ccgtggcacc gaacctgccg gagctcccgc tgaacagctt ggcgccgaaa
     6301 aacagcgtgg cgccgagcag cagcgcgatc gcgccgacca cgaaggcgcc gatcgcggtg
     6361 ggattgggct tgccgctcac gcgctttcgc ccgcgctgcg cgacaggaac cggcgcacca
     6421 gctcgtggtc gctgccggtg agcaggcgtt gcgggtcgcc gtaatcgagc atggtgcggg
     6481 tctccgcgtc gagaaagacc gaattgttac cgatcgcgaa aatgctcgcc agttcgtgcg
     6541 tcaccatcac caccgtggcc cccagcgcat cgcgcatgtg caggatcagc tcgtcgagca
     6601 gccgcgagct gatcgggtcg aggcccgccg aaggctcgtc gaagaacagg atctcggggt
     6661 cgagcgcgat cgcgcgcgcc aggccggcgc gcttctgcat gccgccgctg atttcggagg
     6721 ggtagtactc ctcgaagccc gccagcccca ccagcgccag cttgtacgaa accagctcgc
     6781 cgatggtggc ggcatcgagc ggcgtgtact gctgcagcgg caaggcgacg ttctcggcga
     6841 gcgtcattgc gctgaacagg ccgccgccct ggtaggtgat gccccagcgc cggcgcatgc
     6901 ggtcgcgctc cccgtcgtcg gcgcgctgga agctcgcgcc gccgtagagg atctccccgg
     6961 cggcgggctc gtagaggccg atcatgtgct tgagcagcgt cgacttgccg cagccgctgc
     7021 cacccatgat cacgaagatg tcgccgcggt tcaccgtgaa gtcgagatcc ttctggatca
     7081 cgcgcgagcc gtaggccatg gtgagcccgc ggacctcgat atgcggctgc gcggcgtcca
     7141 cgctcagatc cccagccgct ggtagacgat gttgaacgcc gcgtccgcga cgatcaacgc
     7201 cacgatggcg gtgacaaccg ccctggtcgt tgccttgccc acggcctcgg aactgcgccc
     7261 gcactgtatg cccgcgcgac agccggcgag cgcgatcagc agcccgaaga ccacgctctt
     7321 cgcgagtccc gtcgatatgt gcgtcaggtt cacggcgagc cgcgtctcgt gcaggtactg
     7381 cgtccacgac acgtccatcc ctgtcgccac cagcgcgcca ccgcacatgc cgagcacgcc
     7441 gctgtagagc gtgagcagcg gcatgaccag caccagtgcc agcaggcgcg gcagcaccag
     7501 gaattccatc ggcgacatgc cgagcgtgcg gatcgcgtcg atctcctcgc gcgtctgcat
     7561 ggtgccgagt tgcgcggcat aagccgcgcc cgtgcgcccg gccatgatca cgccggtcat
     7621 cagggcggcc atctcgcgca ccatgccgat gccgaccagg tcggcgacgt agatctgcgc
     7681 gccgaactgg cgcagctgca ccgagccgag ataggcgagg atcatgccca ccagcacgct
     7741 gatcagcgtg acgatgccga tcgcggccgg ccccgcctgc tgcacgaacc agaggaaatc
     7801 gcgcgctcgc gtattggcgc gcccgagaca gagcttgccg agcgcgatca gcagctcccc
     7861 gagaaaggcg agcgccccga gcagctcgcc ccaggcccgc agcagcggct cgccggcaag
     7921 caggcgccgc caccacgggg cgcgcgccgg ccgtgcgtgc tgctcgggcc gcaccgcgcg
     7981 cgccagcgcc atcagccgca gcgcgccctc gggcaggttc gcggaatcga accgcatgcc
     8041 ggcgcgcgtg cattcctcct cgatgccgag cagcagcatc accagcagcg aatcccagct
     8101 gccgagcgcg ttgcagtcga aaccgagctc gcgcgccggc tgtgcgcgca actgcggcag
     8161 cacgtcggcg gcgcgcggcg cgccgcgcgc caccgtccag tcgccggaca gcaccagcgt
     8221 ggttcccccg gcgcgcgcct cgatcgcgca gcgcgccggg ttcggatccg ccgctggtgc
     8281 cgcgctcaaa tccgctgcgc caggttgccg ctgcgctcga gccagtcgcg gcggtcggtg
     8341 gcgcgcttct tcgccagcag catgtcgagc agctggtcgg cctggtcttc ctcgctgagc
     8401 gtgagctcca ccaggcggcg cgtgtccggg gtcatcacgg tctcgcgcag ctgcaacggg
     8461 ttcatctcgc cgagcccctt gaagcgctgc acgttcacct tgcccttctt cttctcggcc
     8521 tcgatcaggt tcagcacgcc ttccttctcg gattcgtcga gcgcgtagta gacgtccttg
     8581 ccgatgtcga tgcgatagag cggcggcatc gccacgaaca cgcgccgcgc ctggaccagc
     8641 gggcggaaat gacgcacgaa cagcgcgcac agcagcgtcg cgatgtgcag cccgtcgctg
     8701 tcggcatcgg ccaggatgca gaccttgccg tagcgcagcc cgccgagatc ggccgagccc
     8761 ggatcgacgc cgagcgcgac ggcgatgttg tggatctcct ccgaggcgag gatctcgctg
     8821 ctctccagct cccaggagtt caggatcttg cccttgagcg gcatgatcgc ctggaacacg
     8881 cggtcgcgcg cctgcttggc cgaaccgccc gcggaatcac cctcgacgag gaacagctcg
     8941 gtgcggtcga gatcctgcgc cgtgcagtcg gcgagcttgc ccggcagcgc cggtcccgag
     9001 gtgatcttct tgcgctcgac ctggcggctc ttgcgcagcc gcgccgcggc ggccgagacc
     9061 gccatatcgg ccagcttgtc gccttcctcg gtgtgttgcc ccagccacag cgagaacgcg
     9121 tcgcgcgcca cgccggcaat gaacgcggcg gcctcgcgcg aggacaggcg ctccttggtc
     9181 tggccgctga actgcggctc gcgcagcttg gccgacagca cgtaggcgca gcggtcccac
     9241 aggtcctcgg ggctgagctt gatgccgcgc ggcagcaggc tcctgaactc gcagtactcg
     9301 cgcagggctt cgagcagacc cgtgcgcaac ccgttgacgt gcgtgccgcc ctgcgccgtc
     9361 gggatcaggt tcacgtagct ctcggtgatc agttcgccgc cctcgggcag ccactgcacc
     9421 gcccagtcca cggcctcggt cttgccgctg aagctgccga cgaagggctc tgccggcagc
     9481 gtcgggtact cggcggtggc gccgcggatg tagtccggca ggccgttttc gtagtaccac
     9541 gcctcgcgct ggcccgtcgc ctcgtcggtg aaggtgatgt tgaggcccgg acacagcacg
     9601 gccttggcgc gcagcaggtg cgtgaggcgc gagaccgaga acttcacggt gtcgaaatag
     9661 cgcggatcgg gcctgaagcg caccgtggtg ccggtgctgc gcctggccac cgagtcgatg
     9721 atctcgagct cggaggcctt gttgccgttc tcgaagcgca tgcgatagac gttgccgtcg
     9781 cgcctgaccg tgacctcgag ctccagcgac agcgcgttga ccaccgacac gcccacaccg
     9841 tgcaggccgc cggaaaactg gtagctgccc tcggagaact tgccgcccgc gtgcagcgtg
     9901 cagaggatca cctcgacgcc gggcttgccc tgctcggggt ggatgtcgac cggcatgcca
     9961 cggccgtcgt cggccaccga cagcgagccg tcggcgtgca gcaccacgtc gatggcgcgc
    10021 gcgaagccgg ccagggcctc gtcgacgccg ttgtcgatca cttcctgcgc gaggtggttg
    10081 gggcgcgtgg tgtcggtgta catccccggg cgcttgcgca ccgggtcgag ccccgtcagt
    10141 acttcgatgg cctcggcatt gtattgggcg ctgactgcca tgcttctccc tgtggtggaa
    10201 cggcgagcgc ggcggatgat acaggagcgg cgcttctcag aggtacccgg ccgagtggcg
    10261 gtcataaccg agctcgacgc cgctcacgcg ctcgacgcgc gtctccaggc gcccgtcggg
    10321 gtgcagttcc agccagcgca ggccgggcgc ctgctcgtcg aggcgaaagc cctcgctgca
    10381 cggggcgaac tggatgcagc tcgagggcga gccgagcagg cgcagccccg cgcgctcggc
    10441 cgagaactcc tggtgcacgt gcccccagag cagcgcgcgt gcctgggggt ggcgggccag
    10501 caccgcgaac agggcgtcgg catcggcgac catctgctcg tcgagccacg cgcagccgat
    10561 cgccaccggc tggtgatgca ggcagaccag cgcgtggcgc gcgggctcgg cgccgagcag
    10621 gcggtccagc agcgccagtt ccgccgcgcc gagactgccg ccgacctggc cggcgacggt
    10681 ggagtcgagc atcacgatct gccagctgcc ggcgcagatg gagcggatca tcgggcggcc
    10741 gcggccgatc acgccgtgca tcagggggcc cgcgtcgtgg ttgcccggca gccagaacca
    10801 cggcaggccg agggcgtcga agctctcgcg cacgcgcacg taggcggcct cggcaccgtg
    10861 gttggcgaga tcgccggtgg cgagcagcag gtcggggcgc aagggtccgt cacgcaccag
    10921 gtcgagcacg tgcgcgaggc tcgcatcggt gtccatgccg agcagggtgc cgccacggcg
    10981 ctcctcgaga tgggtgtcgg tgacctgcac cacgcggaca caggatccgg caagggcgag
    11041 gtcgacgcgc gtgtccgtca tcggccgccg ccttcagccg tcggctgcca gggccgcgga
    11101 gtgcagcgcg tgcccctcgg caaggcagtg cgccagccat tcgccgagca ggcggttgat
    11161 ctgccacttt tcgtcgcgcg ccagcccgtc gcggttgggg tagtcgtagc gcgccagcag
    11221 ccggcgcacg cgcgcgcaac cgacgacttc cgccatgcgc gcgtcgagat aggcccgcac
    11281 cgtgaattcc gcgccgcgcg cgccccagac gggctgctgc tggcgcagca ccacttccgc
    11341 ggtataggga cagcgctgca cgacatgcag gtgcagcacg tccatgccgc cgcggggcag
    11401 gcgcgtgccg atgtgtcgcg cctgtcccgc gacgaaaccc ggcagcagtt tttcgaggcg
    11461 caggaagttc acctcgcatt cggcgtggtg gatctgcagc ggcgaacagg gtgccacggc
    11521 ggcgtcgcgg ccctgcgccg cctggccggc atgtcggggg aagctcatgg agcgaaagta
    11581 tataggcaat tgccgcaggc ccatgacggg gcggcgaaat ttgacggcac aaatcccgcg
    11641 ggtcctgcgc cgtgcattga tcgggcttat taaggatgga cgccctctgc tagactcgcc
    11701 tcgctgccgc cgagctccca atcgatccat gaggcatccg atgctgcatt cccgcctgcg
    11761 cgcgctgatc accacgagcc tgctgctgct gcccgcggcg ctgcgtgccg acacgctgct
    11821 cgacatctac gaactggcgg tgcagaacga cccggtcatc agggcggccg aggccaccta
    11881 tcgcgccggc cgcgagtacg aggtccaggg ccgctcgcag ttgctgccgc aggtgggggc
    11941 gcaggccgag tacggcgagg acgacatgac gcagaagagc acgcgcacca tcgctatcgg
    12001 tgaccagacc ttcaccagca gcagcaatta cgacgtcgag gaggaccagg aaaactatta
    12061 cctcacgctg tcgcagaagc tgttcgacgt gccggcctgg ttcagcttca agcagggcaa
    12121 ggaactcagc aagcaggccg aggcgcaatt cgccgccgac cagcaggcac tgatcgtgcg
    12181 caccgtggag gcctacgtgc tggtgctgcg cgccatcgac aacctgaagt cgagccgcgc
    12241 cgccgaggct gccttccagc gccagctgga gcagacgcag cagcgcttcg acgtcgggct
    12301 gatcgcgatc accgacgtgc acgaagcgcg cgcggccttc gacctcgcgg tggtcgagcg
    12361 cctcaccgac gagggcaacc tgggcgtcgc ctacgaggcg ctgtcggtgc tgaccggcca
    12421 ggaccacgcg aacctgtggc tgctgagcga ggagtttccg gtcgtcgacc cgcagcccgc
    12481 cgccgccgag agctgggtgg agctcgcgat ggaaggcaac ctcggcctga aggtggccag
    12541 ttacgcggcc gaggccgcga tgcagtcctc gcaggccagc cgtgcgcagc acctgcccaa
    12601 gctcagcggc cagctcaccc tgatggacac gtccaccgac ggcgacaacg tggacaacaa
    12661 cctccgtgac ccggcaggca accgcctcaa ccccaggtac cccacctcgg tcgacgtgga
    12721 aggcaacagc gtcatgctga cactgagcat gccgctctac gccggcggtg gcgtgagctc
    12781 gcagcgccgc cagtcctacg agaactacat cgcggcgcgc gaaacgcaca ccagcatcat
    12841 gcgcgacacg atccagttca cgcgctcgct gcatctcttc gtcaccaccg acgtgcagcg
    12901 cgtgaaggcg cgcaggcagg cgatcgtctc ggcgcagagc gcgctggatg ccaccacggc
    12961 gggctacgag gtcggcacgc gcaacgtggt cgacgtgctc aatgcgcagc aggtgctctt
    13021 cacggcgatc cgcgattacg cgaacacccg ctacgactac gtctcgcggc tgcttcgcct
    13081 gcgccagcag gccggcctgc tgagccccga ggacgtggtc gcgctgaaca agtggctggt
    13141 cgaaccgacg cccgccacgg cgaccgcgcc gcggatctga tcgcgcgcgc tatcgcgcgg
    13201 gggattggaa aagtttcccg atcttcgctc gcagtatctg cggcgtgaac ggcttgacga
    13261 catagccgtt cacgccggcc cgtaccgcct cgatgatctg ctcgcgcgtc gcttcggtgc
    13321 tcaccatgag gatcgggatg gcattcagcc gcgggtcggc gcgcaccgcc ctgaccaggt
    13381 cgaggccatc catctgcggc atgtgccagt cggtcagcag caagccgaaa tcgtcctggc
    13441 gcagcagggc gagtgccgtg gcaccgtcat ccgcgagcgt gacgtcgcga aagcccatgt
    13501 cgcgcagcag ttgcccgacg atgcgccgca tgctctcgaa gtcatcgacc acgagaaccc
    13561 tggtgtcgaa gggaatactc atggcgccag catacgcgat cggttcgccc gcccgcagcg
    13621 cggatttgcc agccgtgcgc cgccggcgtt atggtgcgcg ccggtcagca gcaaggcaaa
    13681 cggaacaccg catgggcatc aaactcccgc tcgacatcga cagcgtcaag ggcttcctga
    13741 gcccggtcga aggcgcggca ctctaccgcg ccgcactcga gtgcgcgccg ctcggcccct
    13801 gtctcgagat cggcagctac tgcggcaagt ccacggtgta cctgggcacc gcctgccagc
    13861 gctcgggcgg cgtgctgttc gcggtggacc accaccgcgg ctccgaggag caccagctcg
    13921 gggaggaata tcacgacccg gcgctctacg atggcgcggc ggggctcatg gacagtttcc
    13981 gggagttccg cgccacgatg cgccgcgcgg gcctggatga caccgtggtg cccgtggtcg
    14041 caccgacgcg cgtcgcggcg cgcgcctggc gcacgccgtt gtcgctggtg ttcatcgacg
    14101 gcgggcacag cctggaggcg gcgatggccg attaccgcgg ctggagcggc cacctggttc
    14161 ccggcggcct gctcgcgatc cacgacatct tcccggatcc cgccgacggc ggccaggcgc
    14221 cgtacacgat ctaccggctg gccctggcct cagggctgta cgaagaactg ccgcgcgttg
    14281 agacgcttgg cctcctgcgg cgaatcgctt aaccgcacct cggtcagcag gcggctcgcg
    14341 ccggtgtgcc cggtcagcgc atcggccgcg agcaggatcg caccggcggt ccaggtcggt
    14401 ttctcctcgg gccacatgat gttctcgacc cattgatagc cggtccagta ggcgccgtcg
    14461 tggtcgcgcc actggtgcag ccagctgaac agccgggccg cgcgcgcatg gtcgccggca
    14521 ccgagcagcg ccatcgtgag ctcgcacgat tccgccacgg tcacccacgg ctcgtccgac
    14581 acgcagcgac agcccatgcc ttccttcacg aaggtatccc agcgcgcggc gagccgcgcc
    14641 ttcgcctgcc cgcgcccgag cacgccgccg agcaccgggt agaaccagtc catcgagaag
    14701 cgcgccttgc tctcccaggt ccggtcgaag cgctcggggc ggtggcgcag cgcctcaccg
    14761 aggcgccagc gcgcctcgac ccagcgcggg cagggccggt acagcgcgtg cgcgatgttg
    14821 atcgcgcact ccaggctctt gtggatcgag ctgcagccgg tgaccagcgc gtcgtccatg
    14881 gtgttgcctt cggcatcgac ggcccagcac acctcgccgt gcacggtctg ctgcgagagc
    14941 acgaactcga tcgcgcgctc caccacgggc cagagctcat cgagcagctc gcggtcgccg
    15001 ctcacgaggt agtggtgcca caggcccgtg gcgatgtagg ccacgaaatt ggtctcgcgc
    15061 cgcccgcgct ccagcacctc gccgtccttg taactggccc accacgagcc gtcgggcagc
    15121 tgggtgcggg cgagccagcg ataggcgcgc gccgcggcgt cgaattctcc ggcaatgccg
    15181 aggcccatcg cggcctcgac gtgatcccag gggtcggcgt ggccgcccgg gaaccaggga
    15241 atgcagccat ctgcctgctg ggtcgcgagg atgaaatcca cgctgggacg caggaactcg
    15301 gccgggtaca gccccttgct gagatagagt tcgctcatgg gacaggagcc ttctggaagt
    15361 acatcaccac actcttgccc agcaccgggt tcagcaacgc ctcggcccag cgcgtgaggc
    15421 gcggacgctc catcaggtcc cacaccagca gccggtggta gagcgccacc agccgcgatt
    15481 cgtgctggcg ctcccacagc aggcacttga gccaccagaa cggggcgtgc agggcatggg
    15541 cccaatgccg gcggtagcgc cggaatccca gcgcctcgat gtcgcggttc agccgcgcgg
    15601 cacggaagat gcggatatga ccgccctcgt tggcgtggta ggcatcgctc agcgcccagc
    15661 agaggcgctc gggccagtaa cgcggcacgc tggcgaccag ggcgccaccg ggtttaagca
    15721 cacggcggat ctcggccagc acctcgcggt aggggcctat gtgctccagc acctcggagc
    15781 agatcaccac gtcgaagcag tggtccgcga acggcaactg cagcgcgttg cccactccga
    15841 aacccaggct gcaatgcgcg gtgccgggcc gctccggggc aaacgggcgc gccctggcgg
    15901 cggccgtcgc cacgtcctgc acgctgaggt cgatgcccac gcagtgcacc tcggccctga
    15961 gccacgccgc gatggcgtgg cggccctcgc cgcagcccag atccagcacg cggtcgccgc
    16021 ggcggatcgg cacgtagtca agattgacgg tttccatcgc cggccaggac ctgctcgtag
    16081 taatgcgtca tctgccgcgc cgcccggcgc cagcagaaga gtgtcgacat tcgtgcccgt
    16141 gcacgctgcc ccagttcggt gcgtcgcgcc gggtcggcga gcagcgcagc gatcgccccg
    16201 gcgagcgccg tctcgtcgcc ggccggcacc agcacgccgg catcgcccac cacctcgggc
    16261 agcgcgccgc cgtcggagga gacgaccgcg gcgccgcagg ccatcgcctc cgatgccggc
    16321 aggccgaagc cctcgtacag cgaaggcacc acgaccagcg tggactcgcg gtacaggcgc
    16381 accagctcgg cggtgctgat gccgctcacg aaatcgatgt gggcgccgag atcgagccgc
    16441 gcgatgagct tctcggtatc gccgcccggg cgcggcttgc ccacgaccag cagccgcaga
    16501 cccgggtgcg cgggcagcag cgcggcgaag gcgttcagca gaaccgccag gcccttgagc
    16561 ggctggtcgg ccgaggcggt cgccatcagc tgcagcgnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnacc gccaggccct tgagcggctg gtcggccgag gcggtcgcca
    16741 tcagctgcag cggcttgcgc gcgacgccgg catcgggcgc gaacacctcg gtgtcgacac
    16801 cgttgtgaac cagcgtcagg cgctcggcgg gcaggccgaa atcgcgcgcg atgtcgccgc
    16861 gcgagcactc cgataccgtc accacgtggc gcagcccgcg cgccacgcgc ttctgcatgc
    16921 cgaggaaggt gtgccagcgg cgcgtcagca ggcgcggcca ccaccagcgg caggcggcca
    16981 gcgcgaggcg caggtcgctg gtgatcgggt ggtggatcgt ggtcaccagc ggcacacccg
    17041 cgcgctgcag cgcgagcatg ccgtagctca ggctctggtt gtcgtgcacg atgtcatagt
    17101 cgtcgcggtg ctcgcgcagg tagcgcaacg cgcgccgccc gaaggcctgc ggctcggcga
    17161 agccgccggt cagcttgctc agccactcga tgacgttgct gagcgagcgc aggtggcgcg
    17221 gtcgcagtga cagcaagccg gtctcgtaga ggttcatgct cggcatctcg atcaggcgca
    17281 cgcgcggatc gagatgcgga tagggtggcc cggagatcac atcgacccgg tgaccgagct
    17341 ccagcaaggc cttgctgagg taacgcaggt acacgccctg gccgcccccg taggggtggc
    17401 tgcggtaacc cagcaggcag atgcgcaggg gccggaccgg ctccaggtcc tgcaggctca
    17461 gtgcttccgg gtaacggtcg aggggcaacc gtgccgggtt cagggcttcc atcaagaacc
    17521 tctcgcggac accgggtcga gcacatactg gcgcctgtcg gcgcgcaaag gcgcggattc
    17581 tactgagcgt ccccccgaaa atccatgccg cgggactttg ctggaggaat cgtaatcgtc
    17641 ctgcctctat actccctgcc tgctccgcac aggaaggatc cgggttcatg caacagacga
    17701 attaccgcgg catcgtgctg gcgggcggca gcggcacgcg gctgcacccg ctcaccagct
    17761 cggtcagcaa gcagctgatg ccggtctacg acaagccgat gatctactac ccgctggcga
    17821 cgctgatgct cgccggcatc cgcgagatcc tgatcatcac cacgccgcgc gaccaggacg
    17881 cgttccgcga gctgctgggc gacggttcgc agtggggcct ggagctggtc tacgcggtgc
    17941 agcccgagcc gggcggcctg gcgcaggctt tcctgatcgg cgagcaattc gtgggcgacc
    18001 ggccggtctg cctcgtgctc ggcgacaaca tcttctacgg cggcgggctc agcaagaaac
    18061 tgcagtcggc cgcgctcgag accgacggcg ccacggtgtt cgcctattac gtgcaggatc
    18121 ccgagcgcta cggcgtggtc agcttcgacg cgcagaaccg cgccgtcgac atcgaggaaa
    18181 agccccgaca cccgaagtcg cactacgccg tgacggggct gtatttctac gacaacgagg
    18241 tggtgtccat cgcgaagtcg atccagccct cggcgcgcgg ggagctcgag atcaccgacg
    18301 tcaaccgcat ctacctgcag cgcggccggc tgcgcgtcga gaccctcacc cgcggcgcgg
    18361 cctggctcga taccggcacg cacaattcgc tgctggatgc ctcgaacttc atccgcgtgg
    18421 tcgaggagcg ccagggcctg aagatctgct gccccgagga gattgcctac cgcatgggat
    18481 tcatcacgcg cgagcagctg ctggcgctgg cgcgcccgct gaagaaaagc ggctacggcc
    18541 tctacctgga gcgcatcgcc agcgagggga gcccggtctg atgcagatca tcgccactcc
    18601 cattcccgac gtgctggtgc ttgagccgcg cgtgttcggc gacgagcgcg gcttcttttt
    18661 cgagagctgg aaccagcgcg agttcgcccg cctcggcctg ccggcgcagt tcgtgcagga
    18721 caaccacagc aagtccgccc gcggcatcct gcgcggcctg cactaccaga cgcagcaggt
    18781 gcaggggaaa ctcgtgcgcg tggtcgccgg cgaggtcttc gacgtcgcgg tcgacatgcg
    18841 ccgcggctcg ccgacgctcg gtcgctgggc cggtgcgctg ctctcggcgg agaaccgccg
    18901 catgatgtgg atcccgccgg gcttcgcgca cggcttctac gtcaccgcgg actcggccga
    18961 gttcatctac aagtgcacgg acttctacgc ccccgagcac gaggtgtcgc tgcgctggga
    19021 cgacccggcg atcggcatcg actggccgct catcgacggc aagccgcc
//
LOCUS       DAORUQ010000037        19052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2515_length_19052_cov_6.286729, whole genome shotgun sequence.
ACCESSION   DAORUQ010000037 DAORUQ010000000
VERSION     DAORUQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19052
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2515_length_19052_cov_6.286729"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLW13_05235"
     CDS             complement(<1..348)
                     /locus_tag="PLW13_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQY69807.1"
                     /translation="MEDSFRESSIRYHTDYPPGKLAIQPTKPMANKHDLARAYSPGVA
                     YACELIEQDPNQAANVTARGNLVAVVTNGTAVLGLGDIGPLAGKPVMEGKAVLFKKFA
                     NIDVFDIEIDEKNV"
     gene            complement(451..783)
                     /locus_tag="PLW13_05240"
     CDS             complement(451..783)
                     /locus_tag="PLW13_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HQY69808.1"
                     /translation="MRKMLKVLLTAAAITAASAALALDVQEAKSAGWVGEQRDGYLGL
                     VAASAPPEARQLLTEINQARRASYREIAARNGIELRAVELLAAEKALQKTPPGQFIQA
                     EDGSWARK"
     gene            complement(834..1025)
                     /locus_tag="PLW13_05245"
     CDS             complement(834..1025)
                     /locus_tag="PLW13_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HQY69809.1"
                     /translation="MHFRHTLLVLLALLAAAACTPTVKVEAPEKPITINLNVKIEHEI
                     RVKVDRELESLFEDDNGLF"
     gene            complement(1163..3430)
                     /locus_tag="PLW13_05250"
     CDS             complement(1163..3430)
                     /locus_tag="PLW13_05250"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HQY69810.1"
                     /translation="MNRRRLRSALLVAAAALLLGALALTLWLRQHPESVIAALNARLP
                     PGIALGELHGLRVSALGGRVELLTLEANGTALRIEDARWFWRITGIWPLGIEAQRVSM
                     RALGVRLAATDSTTLLPRFWLTDWWPLVARMSAASEQLRIDDSEGARLLDGRIDASDG
                     GASGGATLRVPGVPALALQWRPHADLPRAWVIDWHGETSDGPNGSLLLQATPDGADWQ
                     LEARLPALQASGLQLSGLTLSAQGSSDLFEGSGPLASARLQLGGKADTPRGEASVQCT
                     GALQMQQSRQSTLDVTACEAALAGATLALAAPLALALDAALAPERLDTAGGTVKLRGL
                     RQAPWTLEDLQLTLHKGTLWQASPTALALPNAEMAATIAHAGSDAHATLHGRLTGLSA
                     HGENRGAKLEGKLELRQGAHVLKALDLDTRFDANPEKLRLEGALSRARPGKLLAWQGD
                     YAFADATYRATLTLDSSGWDWGKGLLRTLLGAKQTLVPGDLLGGELRATAMVRGAPER
                     MRIAIDASARRLAGIAAGMGVAGLDCGPISLTLENGVIGAFPDLPCTIDSVNAGITLN
                     RLRATLRHGAPGWSLHDVSGELFGGSFGAASIGPLGPAPIDTSVSLHAIDLTRVGKLL
                     DDPDLTLTGALDGTLPVHIEGGTFTVRQGEVHSTAPGVIRYRPATPPAEESAQIALSR
                     KALSNLEFDTLEATLDYATDGQLALAARIRGRNPDLDAKRPVHLNLTLETNLRTLLRS
                     LTTGDRISEWLEKRM"
     gene            complement(3495..4268)
                     /locus_tag="PLW13_05255"
     CDS             complement(3495..4268)
                     /locus_tag="PLW13_05255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69811.1"
                     /translation="MNTATSHSPRSGVIVTGGASGIGLASAEALAAAGRPVAIWDLRA
                     TDAERVANDIASRFGVTCIGIGIDLRATDGIDAAVAATRAALHSIGGLVHAAGVSMAI
                     PLEELDVPQWDTVLNVNLRALPFVVKAIRADLAANAGSAVVGIASINATLGNWMNPAY
                     TASKGGMLSLVRSLADDLGRVGVRVNAVSPGQIRTPMMQPVLDHLSDEAFTKRILLGR
                     IGEPREIAAVVRFLLSDEASYVTAAEIVVDGGNISSQRV"
     gene            4537..4983
                     /gene="msrB"
                     /locus_tag="PLW13_05260"
     CDS             4537..4983
                     /gene="msrB"
                     /locus_tag="PLW13_05260"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008010532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQY69812.1"
                     /translation="MWSLGGATQALERVRKTRDEWKALLPAPAFAVLFEEATEPAGSS
                     PLNDEKRKGTFVCAACFLPLFDSAHKFDSGTGWPSFFDVLPGAVGTRRDFKLILPRTE
                     YHCARCGGHQGHVFEDGPQPTGLRYCNNGVALRFVPAGEALPELRT"
     gene            4980..5594
                     /gene="msrA"
                     /locus_tag="PLW13_05265"
     CDS             4980..5594
                     /gene="msrA"
                     /locus_tag="PLW13_05265"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQY69813.1"
                     /translation="MKRPGLAKLSSALVLAMAVVCPVLAAPAPAAGATAVFAGGCFWC
                     IEADFEKLPGVLDVESGYTGGYVPDPDYEQVSAGGTGHAEAVRVRYDPARVSYAQLLD
                     YFWKHIDPTVKDRQFCDAGNQYRSAIFPATDEERRAAEASRDALLASGRFAQVLTTIE
                     PAAAFHPAEDYHQDYYRKNPLRYAYYRRSCGRDARVEELWGSAR"
     gene            5678..6754
                     /locus_tag="PLW13_05270"
     CDS             5678..6754
                     /locus_tag="PLW13_05270"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQY69814.1"
                     /translation="MQTPYAPLKGRAALGNPAGRFERQVRERCDDGWAQPGEDAPRVL
                     QEVLPEQARTIIARNSSPDVPFSQSVNPYRGCEHGCIYCYARPSHAYIDLSPGLDFER
                     RLRFKANAAEVLERELGNPRYVCEPITLGANTDPYQPVEAGLGITRRLLEVCERHAQP
                     VSIITKSALVLRDIDLLARMAARGLCQVMLSVTTLDDALKRRLEPRTASGAARLRAVR
                     ALADAGVPVGVLVAPVIPMINDAELEAILAASADAGARKAAWILLRLPWEVRELFREW
                     LQCHYPERAGHVMSLIRQSREGRENDPRFGSRMRGSGPYADLIARRFTVAARCCGLAG
                     EGWPALDCTQFTGAPRPARQLQLW"
     gene            6859..9489
                     /locus_tag="PLW13_05275"
     CDS             6859..9489
                     /locus_tag="PLW13_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY69815.1"
                     /translation="MKLSIRSIALLMILVPMLLPALGLSAWLTSLRVSDARAELESRG
                     ERESRYLAHASELALLVADSETLQRLAASNLKGAGAAVATLFLDAEGAVLAAAGAASE
                     VDMARQCWRDAAGCAGSEQRHVFQREVRAHMRAARDEAVAFSAVDAAAEPELIGRVLL
                     SFDPHGLAAIQRAMLLNSTVITLGALLVAWALAHVVSRRLTVPMHRLSLVVARIRAGE
                     LGARTQPTGSGEMRELEDGVNAMADWVEAANAEQNRRVDEATAELSRTLNEREQRNRE
                     LDQALERAEAAGRAKSMFLARMSHELRTPLTTVTGFARMMQQAESPEQRAELFRHVEQ
                     GSRTLQALVDDILIVETIDNDSLRLERREFDLESCIEDTVMMQAPAAHARALALVCHV
                     EPGLPQRVIGDSLRIAQVLGNLISNAIKFTEVGHVTVRASADVPAAGDARLLLEVTDT
                     GIGIAPGNVDWLFQPFAQADESMTRRFGGSGLGLSISLGLARALGGQLTLNGAPGVGT
                     TARLELPLELAPSRAGEGEDLPAPPRLRVALLCRSDSPHVPVLRDYLGSRFELAGVHD
                     LRMIPVGPGAFGAVDLLLALEDTGEPCCEPWLGGATPALRLRPIEVVTDPAQRYVADL
                     VPLPLRRRQLLAACARRAGAPTQIAEGHPAAGVPWQPDIDLHCLIVEDNELNRHMIAM
                     RLRDFGVTVSEAGGGAEALRLLPGAAPDLVLLDVHMPGMDGVTLARHLRQRHPGLPVY
                     ALTANVIGSEEAALAAAGVRGVLYKPIDEAKLLEVLRRHARRDDGWDIEVRAGITRDE
                     VAAELRRLLRAVEACVAARDLAGGVDAAHQLLGVARMFTRGVLAERCLALETALRDNG
                     GEAAHGTLHRLALLLGPAGA"
     gene            9559..11733
                     /locus_tag="PLW13_05280"
     CDS             9559..11733
                     /locus_tag="PLW13_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HQY69816.1"
                     /translation="MSHGRMLLSCAILLVALLGCSDEHPGRAPARGDAAAVATPVPAV
                     TSVDGARIAAADDEPHNWLSHGRSYSEQRFSPLGHIDAANVGRLGLAWFFDFPTDRGM
                     EATPIVVDGRLYVTGTWSMVYAFDAKSGALLWHHDPGVPRQWAVHLCCDAVNRGVAAW
                     GGKVFVGTLDGRLQALDANDGKVLWSVQTTPRDKPYSITGAPRVVKGLVIIGNGGSEL
                     GVRGYVSAYDAETGDMKWRFYTVPGDPAQPFESPALERAAATWSGGEWWKIGGGGTVW
                     DSMAYDPELDLLYVGVGNGSPWNQQYRSPGGGDNLYLSSIVALDPDTGAYAWHYQTTP
                     GDTWDYTATQHLVLAELPIGGTPRKVIMQAPKNGFFYVLDRATGEFLSGDAYAYQTWA
                     EGIDANGRPIPNAQARYVGKEALVFPSPHGAHNWHPMSFSPRTGLVYIPVQEIPFVYK
                     HDDKFAFEPGLWNTGVDVEAAGLPEDPAAAAEVLKLVQGHLVAWDPVARREAWRVEQG
                     APFNGGVLSTAGDLVFQGSARGEFVAYRADTGERLWAAPVQTGVVAAPMTYAVDGEQY
                     VAVVVGWGGGLPLAGGRVAQMAGSVNRSRLLVYALDGRQQLPEPVAAARALAPPPLAA
                     TPGQVARGKTLYAARCFACHGDGAVGGGVVPDLRYASAATHAEFDAIVRGGLRHQKGM
                     VGFAQVFGREDVEAIHAYVIKRAHDLLTEQAAQAARQGGDAG"
     gene            complement(11780..13240)
                     /locus_tag="PLW13_05285"
     CDS             complement(11780..13240)
                     /locus_tag="PLW13_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HQY69817.1"
                     /translation="MLWLCLRFPLLPLEVFTRADMHAQRPVIVTEKHRVLCHDALAGE
                     HGVSPGISASTAQALCPGLLTLERRKEREAAALLGLADWGYRFTSLLALEPPDSLLLE
                     VSGSLRLFGGLERLLRRVEDELSERGHAHLDGLAPTPAGAALLARARPCTPADIPPWC
                     ANGDLAAFRAGLGELPLDLLEVPDRQRLRMQRMGFSRIGALLALPRAALGKRFGHEFL
                     DRLQRLGGEKPDPRLPHQPRDFFHAELQFLEPLHHSTMLLFPAQRLLRELGQFLDRRQ
                     CFCQRLDWRLRDISGRTLALDLPCSLAHNSHAALIGLTRLKFESLRLAEPAESLALVC
                     RDFAPVHQQSAALFPDADEDAEQAGRLLLDRLAIRLGEGCVHGLGTADAHLPEHAWRS
                     TQGGGGAASGATAIPAQRPSWLLPEPLPLARDAQGLRWHGRLRLLSGPERIESDWWAT
                     EGKRDYFIARHEQNGTLCWVFRDHHSRRWFLHGLFG"
     gene            complement(13253..14068)
                     /gene="imuA"
                     /locus_tag="PLW13_05290"
     CDS             complement(13253..14068)
                     /gene="imuA"
                     /locus_tag="PLW13_05290"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HQY69818.1"
                     /translation="MNRLLQTLLARPDVWQASRPRAVTATGDTLPSGHAALDRVLHRG
                     GWPRAALTEVLSGSCGIGEMQLLAPALAHCSRGTRRLFFVCPPYLPYAPALLAQDVAL
                     ERLLVVRAERDVDTLWCAEQILRSGAAACLLAWLPESRLGDYRALRRLQLAAQGSASL
                     AFLFRPPQAAHSLSPAALRIVLRGTREHLALEIVKQRGGQAGQQLTLARANALLQPRI
                     DPALLPATVDQAPVGPALSGQMPCPDKSGPTAERMPCPDKSGPTTTPAGTALH"
     gene            14430..15062
                     /locus_tag="PLW13_05295"
     CDS             14430..15062
                     /locus_tag="PLW13_05295"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQY69819.1"
                     /translation="MLQQLTTMLEMQDRMNRKVHPDWINQQFAWYRALWIECGELIEH
                     YGYKWWKHQQPAWEHVKLEIVDIWHFGMSMRFDGTSTPAQIAAGMLEELRLAPPQTME
                     LREAVEALAGRALVGREFPVGHFWSLLQAAGMTPDELFTAYVGKNVLNFFRQDHGYKD
                     GSYRKTWQGREDNEHLAELLGQLDSRSPRYADELYGALAARYAQAAAANA"
     gene            15312..16211
                     /locus_tag="PLW13_05300"
     CDS             15312..16211
                     /locus_tag="PLW13_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69820.1"
                     /translation="MPGTEQYLSGSALARRLNMPSRELFTLLAEQGWIVRQGEQWRLT
                     RKGEFEGGRYLNSERFGTYIGWPEQVLEHRLFSVSPEQTLLNAAALGLRVGLSARQTN
                     LLLLELGWIRKGVKGWELTARGKALGGVQEEYPDTGVPYVRWPATLPGNPVLNENLQV
                     IHAYEHIGEAAERDLFGDAEREIRVDGGDGLRALDGHVLHSKAELMICHWLYMSEIAH
                     AYHRRLPVEGDYRCDFYLPSLQLYIEYWGDEAAPGQLTAKLNKKAVYERHGLKLLEIG
                     PDDLARLDEVLPRALLKHGLAVY"
     gene            complement(16218..16526)
                     /locus_tag="PLW13_05305"
     CDS             complement(16218..16526)
                     /locus_tag="PLW13_05305"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQY69821.1"
                     /translation="MRAQKLDDATITARLAALPGWSLRDGKLRCAYRFADFSAAFAFM
                     TRSALRAEQLDHHPEWFNVYNRVEIALTTHDAHGITALDFTLAEAMDGFARALGSTAA
                     "
     gene            complement(16548..17651)
                     /gene="trmA"
                     /locus_tag="PLW13_05310"
     CDS             complement(16548..17651)
                     /gene="trmA"
                     /locus_tag="PLW13_05310"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HQY69822.1"
                     /translation="MRYSEQDYSAPACAALLEQKVRVVHDAMRELGAPAPEVHASPAR
                     HYRQRAEFRFWHEGDDAFYAMFDPANPREPVRIDEFPPASQRINALMQRLRAAVLRSP
                     ALKRKLFQVEFLSTLANEALVVLVYHRPLDAAWEDAARELSLALDAQLIGRSRGQRIV
                     IGESFVTERLQVDGRELVLRQPEGCFTQPNAEVNRAMLSWARAGCGAARGGLLELYCG
                     IGNFTVALADRFDTVLATEINKVAIAAAQYNLAANGIDNTFIARLSSAEASAALARER
                     PFRRLRDFDLDRFEAGTVLVDPPRAGLDARTLAQVAGFGRIVYISCNPQTLRENLVAL
                     AATHDIERFALFDQFPYTHHLECGVTLQRRNAT"
     gene            complement(17648..18685)
                     /locus_tag="PLW13_05315"
     CDS             complement(17648..18685)
                     /locus_tag="PLW13_05315"
                     /inference="COORDINATES: protein motif:HMM:NF006564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HQY69823.1"
                     /translation="MSKPMHLSAPPSSCPEEHPFAPFVRTLGRGRRGARSLEREEART
                     ALGMILDGEADPLQTGAFLMLLRVKEETAEEIAGFVQAARERAQLPEAPIIVDLDWPT
                     YAGKRQQPPWYLLSALLLATHGHRVLMHGCEPHARNRLFAAQALPALGIAAARDWQEV
                     REALDTRRFAYAPLALFSPGLQALLNLRHLFGLRSPVNTFVRHMNPLAAPATMHSLQH
                     PAYAVLHAGAARQLGQPRALIFRGEGGECELRPNADSRCILLEGDAQRELGFTRQLAE
                     RGDAAIVPDVALLPALWNGRTEDAYGEAAVIGTAAAALLALGGANDADAALALARDYW
                     RRRGPWPGDTP"
     gene            complement(18682..19008)
                     /locus_tag="PLW13_05320"
     CDS             complement(18682..19008)
                     /locus_tag="PLW13_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HQY69824.1"
                     /translation="MKANAALPPRDADGFLLDRADWNEQVATALAREEGLTLTPAHRE
                     ILHMLQAYYREHEHAPAMRALVSLTRRTLGPDKGRSVYLLGLFPGSPARVAAKIAGLP
                     RPEHCL"
BASE COUNT         2805 a         6342 c         6979 g         2926 t
ORIGIN      
        1 gacgttcttc tcgtcgatct cgatgtcgaa gacgtcgatg ttggcgaact tcttgaacag
       61 gaccgccttg ccctccatca ccggcttgcc ggccagcggg ccgatgtcgc cgaggccgag
      121 caccgcggtg ccgttggtca cgacggcgac gaggttgccg cgcgccgtga cgttcgcggc
      181 ctggttgggg tcctgctcga tcagctcgca ggcgtaggcc acgccgggcg agtaggcgcg
      241 cgcgaggtcg tgcttgttcg ccatcggctt ggtcggctgg atcgccagct ttcccggggg
      301 gtaatcggtg tgatagcgaa tcgaactttc gcggaaagag tcttccatcg ggcgatgctc
      361 cggcacggtg agccgggcac tcggtggcgc ccggccgggg gaaaggaggc gggattatag
      421 agagcgtggc ccgtgaaggc cacgccaggg ctatttcctg gcccagctgc cgtcttcggc
      481 ctggatgaac tggccgggcg gggtcttctg cagcgccttc tcggcggcga gcaactcgac
      541 ggcccgcagc tcgatgccat tcctcgcggc aatctcccgg tagctggcgc gtcgcgcctg
      601 gttgatctcg gtgagcagtt gcctcgcctc gggcggcgcc gaggcagcga cgagaccgag
      661 gtagccgtcg cgttgctcgc cgacccagcc ggcgctcttc gcttcctgca cgtcgagcgc
      721 gagggcggcg ctggcggcgg tgatcgcggc ggcggtgagc agaactttga gcatcttgcg
      781 catggtcgtc tccagacatc gccgggcgcg tgttcgcgcc cggggcagct tcctcagaac
      841 aggccgttgt cgtcctcgaa cagcgattcg agctcgcggt ccaccttgac gcggatctcg
      901 tgctcgatct tgacgttcag gttgatcgtg atcggttttt cgggggcctc gaccttcacc
      961 gttggcgtac aggcggctgc ggccaggagt gccaggagca caagcaacgt gtggcgaaaa
     1021 tgcatcgcgt tctccatgtg gcgtatcgcg tctgtccctt gcgccgcagg tcggcattca
     1081 tgccgacggg tacctgcgtt gatccggcgg gttcggcttc gccgaaccct tgtcgggctg
     1141 aagcccgacc tacgaccccg ccctacatcc tcttttcgag ccactcgctg atgcggtccc
     1201 cggtggtcag gctgcgcaac agggtgcgca agttggtctc cagagtcaaa ttcaggtgca
     1261 ccggcctctt ggcgtccagg tccgggttgc gcccgcgtat ccgggccgcc agcgcgagct
     1321 ggccgtccgt ggcgtaatcg agcgtcgcct ccagggtgtc gaactccagg ttcgacagcg
     1381 ccttgcggct gagcgcgatc tgcgccgatt cctccgcggg tggcgtggcc gggcggtatc
     1441 ggatcacgcc cggtgccgtg ctgtgcactt cgccctggcg caccgtgaac gtgcctccct
     1501 cgatgtgtac cggcagcgtg ccgtccagcg cgccggtgag ggtcaggtcg gggtcgtcca
     1561 gcagttttcc gacccgggtg aggtcgatcg cgtgcaggct cacgctggtg tcgatggggg
     1621 ccgggccgag cggcccgatg gacgcggcgc caaagctgcc gccgaagagt tcgccgctca
     1681 cgtcgtgcag cgaccagccg ggcgcgccat ggcgcagtgt cgcgcgcagg cggttcagcg
     1741 tgatgccggc gttgacgctg tcgatggtgc agggcaggtc ggggaacgcg ccgatgacgc
     1801 cgttctcgag ggtgagcgag atcggcccgc agtcgaggcc ggcgacgccc atgcccgcgg
     1861 cgatgccggc gagccgccgc gcgctggcat cgatcgcgat gcgcatgcgc tcgggcgctc
     1921 cgcgcaccat ggcggtggcc cgcaactcgc cgcccagcag atcgccgggc accaaggtct
     1981 gcttcgcgcc gagcagcgtg cgcagcagcc ccttgcccca gtcccagccg gaggaatcca
     2041 gcgtcagcgt ggcgcggtaa gtggcgtcgg caaaggcgta gtcgccctgc caggcgagca
     2101 gcttgcccgg cctggcgcgc gatagcgcgc cctcgaggcg cagcttttcg gggttcgcgt
     2161 cgaagcgggt atcgaggtcg agcgccttca gcacatgggc gccctggcgc agttcgagct
     2221 tgccctcgag cttcgcgccc cggttctcgc catgggcgct cagtccggtc aggcgtccgt
     2281 gcagcgtggc atgcgcgtcg ctgccggcgt gggcgatcgt cgcggccatt tccgcattgg
     2341 gcagggcgag cgcggtgggg gacgcctgcc acagcgtgcc cttgtgcagc gtcagctgca
     2401 gatcctccag cgtccacggc gcctgcctca gtccgcgcag cttcaccgtg ccgccggcgg
     2461 tgtcgagccg ctcgggcgcg agcgccgcgt cgagcgcgag ggcgagcggt gctgccagcg
     2521 cgagcgtcgc gccggcaagc gcggcctcgc atgccgtgac gtccagcgtg gattgccgcg
     2581 actgctgcat ctgcaacgcg ccggtgcact gcacgctcgc ctcgccgcgc ggcgtatcgg
     2641 ccttgccgcc gagttgcagc ctggctgagg cgagcgggcc gctgccctcg aacaggtcgc
     2701 tgctgccctg cgcggacagc gtcaacccgc tgagctggag cccggaggcc tgcagcgccg
     2761 gcaaccgtgc ctcgagttgc cagtcggcac cgtcaggcgt ggcctgcagc agcaggctgc
     2821 cattcggacc gtcgctggtc tcgccgtgcc agtcgatcac ccaggcccgg ggaaggtcgg
     2881 cgtgcggtcg ccactgcagt gcgagcgcgg gaacgccggg gacgcgcagc gtggcgccgc
     2941 cgctcgcgcc gccgtcgctg gcgtcgatac gtccgtcgag cagccgggcg ccctcgctgt
     3001 cgtcgatgcg caactgctcg ctggccgcgc tcatgcgggc caccagcggc caccagtcgg
     3061 tgagccagaa gcgcggcagg agcgtcgtgc tgtccgtcgc cgcgagtcgc acgccgaggg
     3121 cgcgcatgct cacccgctgc gcttcgatgc cgagcggcca gatgccggtg atgcgccaga
     3181 accagcgcgc atcctcgatg cgcagcgccg tgccgttcgc ctccagcgtg agcagttcta
     3241 cccgcccgcc caaggcggat acccgcaggc cgtgcagctc gccgagcgcg atgcccgggg
     3301 gcaggcgcgc attgagtgcg gcgatgacgc tttcggggtg ctggcgcagc cacagcgtca
     3361 gcgccagcgc gcccagcagc agcgcggcgg cagcgacgag cagggcggaa cgaagccggc
     3421 gccggttcat gcgcaagtcg tggcgcgttc gagcgctcgt tgcgccgtgg ccggcaccgc
     3481 gttgcgtgcc gcgctcagac gcgttgcgag gagatgttgc cgccgtctac cacgatttcc
     3541 gccgccgtga cgtagctggc ctcgtccgac agcaggaagc gcaccaccgc ggcgatctcg
     3601 cgcggctccc cgatacgccc gagcaggatg cgcttcgtga aggcctcgtc cgagagatgg
     3661 tcgagcacgg gctgcatcat cggcgtgcgg atctggcccg gggagacggc gttgacgcgc
     3721 acgccgacgc gcccgaggtc gtcggcgagc gagcgcacca gcgacagcat gcctcccttc
     3781 gaggcggtgt aggcggggtt catccagttg cccagcgtgg cgttgatcga ggcgatgccg
     3841 accacggccg agcccgcgtt ggccgcgaga tcggcgcgga tcgccttcac cacgaagggc
     3901 agcgcgcgca ggttcacgtt gagcacggtg tcccactgcg gcacatcgag ctcttcgagc
     3961 gggatggcca tcgacacgcc ggctgcgtgc accagtccgc cgatcgagtg cagcgcggcg
     4021 cgcgtggcgg cgaccgccgc atcgatgccg tccgtggcgc gcaggtcgat gccgattccg
     4081 atgcaggtga cgccgaagcg cgaggcgatg tcgttcgcca cgcgctccgc gtcggtggca
     4141 cgcaggtccc agatcgcgac cggccgtccg gccgcggcga gcgcctcggc cgaggcgagg
     4201 ccgatgcccg aggcgccgcc ggtgacgatc acgccactcc gtggcgaatg cgatgttgca
     4261 gtgttcacga tgggtactcc gggtcgcaag ttcgaggccg cgcaggatcg cttcaggcgg
     4321 gcgctcgcgt caacggttga ttccgccgct cgcaggcggg tagtcgtgca agcagccggt
     4381 ccggaacttt cggcgctgca tgggcgtcac agtaacggca tcgcgaacac gcgcccgcgc
     4441 cgcggtggct gcgggcatca tggcattgca ggagaagggc aggtgaagcg acgggatctt
     4501 gtgcgagggg tgcagcggct gacggcggcg ctggcgctgt ggtcgctggg tggtgcgacg
     4561 caggcgctcg agcgcgtgcg caagacgcgc gacgagtgga aggcgctgct gccggcgccg
     4621 gctttcgcgg ttctgttcga agaggccacc gagccggcgg gcagcagccc gctgaatgac
     4681 gagaaacgca agggaacctt cgtgtgcgcg gcctgcttcc tgccgctgtt cgacagcgcg
     4741 cacaagttcg acagcggcac cggctggccg agtttcttcg acgtgctgcc gggcgcggtg
     4801 ggcacccggc gcgacttcaa gctgatactg ccgcgcaccg agtaccactg cgcgcgctgc
     4861 ggcgggcacc agggtcatgt cttcgaggac ggcccgcagc ccacgggact gcgctattgc
     4921 aacaacggtg tcgcgctgcg cttcgtgccg gcgggcgagg cgctgccgga gttgcggaca
     4981 tgaagcgacc cggtttggcg aagctttctt ctgcactggt actcgcgatg gctgtcgtgt
     5041 gcccggtcct ggccgcgccg gcgccagcag cgggtgccac cgcggtattc gcgggcggct
     5101 gcttctggtg catcgaggcc gacttcgaaa aattgcccgg ggtgctggac gtggaatcgg
     5161 gctacaccgg tggctacgtg cccgacccgg actacgagca ggtcagcgcc gggggcacgg
     5221 gtcacgccga ggcggtgcgc gtgcgctacg acccggcacg cgtgagctac gcgcaactgc
     5281 tcgattactt ctggaagcac atcgacccga cggtgaagga ccgccagttc tgcgacgcgg
     5341 gtaaccagta ccgcagcgcg atcttccctg ccacggacga ggaacggcgt gccgccgagg
     5401 ccagccgcga cgcgctcctc gcctcgggcc ggttcgcgca agtgctcacc acgatcgagc
     5461 cggccgcggc attccacccg gccgaggatt accaccagga ttactatcgc aagaacccgc
     5521 tgcgctacgc gtattaccgg cgcagctgcg ggcgcgatgc gcgggtcgag gagctctggg
     5581 gcagcgcgcg ctgaggcgcg atgccgtccg gctgtacagg aaagctgtat acctatacag
     5641 ttaccgggtg tgatcccgcc accaggaggc gcacccgatg caaacccctt acgcacccct
     5701 gaagggacgc gcggcgctgg gcaacccggc ggggcgcttc gagcgccagg ttcgcgagcg
     5761 ctgcgacgat ggctgggcgc agcccgggga agacgcgccg cgcgtcttgc aggaagtgct
     5821 gcccgagcag gcgcgcacga tcatcgcgcg caacagctcg cccgacgtgc ccttcagcca
     5881 gtcggtcaat ccctatcgcg gctgcgagca cggctgcatc tactgctacg cgcggcccag
     5941 ccatgcctac atcgacctgt cgccggggct ggatttcgag cgccggctgc gcttcaaggc
     6001 caacgccgcc gaggtgctgg agcgcgagct cggcaatccg cgttacgtct gcgagcccat
     6061 cacgctggga gccaacaccg atccctatca gcccgtggag gccgggctcg gcatcacgcg
     6121 gcgcctgctc gaggtctgcg agcgccacgc gcagccggta tcgatcatca ccaagtccgc
     6181 gctggtgctg cgcgacattg acctgctggc gcgcatggcc gcgcgcggcc tgtgccaggt
     6241 gatgctcagc gtgaccacgc tggacgatgc gctgaaacgc cggctcgagc cgcgcacggc
     6301 ctcgggtgcg gcgcgcttgc gcgcggtgcg cgcgctcgcc gacgcgggcg tgccggtcgg
     6361 ggtgctggtt gcgccggtga tcccgatgat caacgacgcc gagctggagg cgatactcgc
     6421 cgcgagcgcc gacgcaggcg cgcgcaaggc cgcgtggatc ctgctgcgtc tgccgtggga
     6481 ggtgcgcgag ctgttccgcg agtggctgca atgccattac cccgagcgtg ccgggcacgt
     6541 gatgagcctg atccgccaga gccgcgaggg gcgcgagaac gatccgcgct ttggttcgcg
     6601 catgcgcggt tcgggaccgt atgccgacct gatcgcgcgc cgcttcacgg tggctgcgcg
     6661 ttgttgcggc ctggccggcg agggctggcc ggcgctcgac tgcacgcagt tcaccggcgc
     6721 gccgcggccg gcgcgtcagc ttcagctctg gtgagagcct gagtatgttt cgctgcctgc
     6781 tgcgcacgct gaagtgttct caggccctga gtccttgcgc tatgctcggc cccggactga
     6841 gccgcaacaa caccgacaat gaaactcagt atccgttcga tcgcgctgct gatgatcctg
     6901 gtcccgatgc tgctgccggc gctgggcctg agcgcgtggc tcaccagcct gcgcgtgagc
     6961 gatgcccgcg ccgagctgga gtcgcgcggg gagcgcgagt cgcgctacct cgcccacgcc
     7021 agcgagctcg cgctgctggt cgccgacagc gagacgctgc agcgcctggc ggcgagcaac
     7081 ctcaagggcg cgggcgcggc ggttgccacg ttgttcctcg atgccgaggg ggccgtgctg
     7141 gcggccgccg gcgcggcgtc ggaagtcgac atggcccggc aatgctggcg tgatgccgcc
     7201 ggttgcgcgg ggagcgaaca acgccacgtg ttccagcgcg aggtgcgcgc gcacatgcgc
     7261 gcagcgcgcg acgaggccgt ggcgttcagc gccgtggatg ccgccgccga gcccgagctg
     7321 atcggccggg tgctgttgtc cttcgatccg cacgggctgg cagcgatcca gcgcgcgatg
     7381 ctgctgaaca gcacggtgat cacactgggc gcgctgctgg tggcctgggc actggcgcac
     7441 gtggtatcgc gccggctcac ggtgcccatg caccgcctgt cgctggtggt ggcgcgcatc
     7501 cgcgccggcg agctcggggc gcgcacgcag cccacgggaa gcggcgagat gcgcgagctc
     7561 gaggacggcg tcaacgcgat ggccgactgg gtggaggcgg ccaacgcgga gcagaaccgg
     7621 cgagtcgacg aagccaccgc cgagctctcg cgcacgctga acgagcgcga gcagcgcaac
     7681 cgcgagctcg accaggcgct ggagcgcgcc gaggcggccg ggcgcgccaa gagcatgttt
     7741 ctcgcgcgca tgagccacga gctgcgcaca ccgctgacca ccgtgactgg attcgcgcgc
     7801 atgatgcagc aggccgagag ccccgagcag cgcgccgagc tgtttcgcca cgtcgagcag
     7861 ggctcgcgca ccctgcaggc actggtcgac gacatcctga tcgtcgagac gatcgacaac
     7921 gactcgctgc gtctggagcg gcgcgaattc gatctcgagt cctgcatcga ggacacggtg
     7981 atgatgcagg cgccggccgc gcacgccagg gcgctcgcgc tggtctgcca cgtcgaaccg
     8041 gggctgccgc agcgcgtgat cggcgattcg ctgcgcatcg cgcaggtgct cggcaacctc
     8101 atcagcaatg ccatcaagtt caccgaggtc gggcacgtga cggtgcgcgc cagcgccgat
     8161 gtcccggcgg ccggcgacgc gcgcctgctg ctcgaggtga cggataccgg catcggcatc
     8221 gcgcccggga acgtggactg gctgttccag cccttcgcgc aggccgacga gagcatgacg
     8281 cggcgctttg gcggcagcgg cctcgggctg tcgatcagcc tggggctggc gcgcgcgctc
     8341 ggcggacagc tcacgctgaa cggcgcaccg ggcgtgggta ccaccgcgcg cctggagctg
     8401 ccgctggagc tggcgccatc gcgcgccggg gagggcgagg atctgcctgc gccgccacgc
     8461 ctgcgcgtgg cgctgttgtg tcgcagcgac agcccccacg tgccggtgct gcgcgattat
     8521 ctcggcagcc gtttcgagct ggcgggcgtg cacgatctgc gcatgatccc ggtcgggccc
     8581 ggcgcgttcg gcgcagtcga cctgctgctg gcgctggagg acacgggaga gccttgttgc
     8641 gaaccctggc tcggcggtgc gacgccggca ctgcgcctgc ggccgatcga ggtggtcacg
     8701 gacccggcgc agcgctacgt cgccgacctg gtgccgttgc cactgcggcg gcgccagttg
     8761 ctcgccgcct gtgcccgccg tgccggtgcc ccgacgcaga tcgccgaggg gcatcccgcg
     8821 gcgggcgtgc cctggcaacc cgatatcgat ctgcactgcc tgatagtgga ggacaacgag
     8881 ctcaaccggc acatgatcgc gatgcggctg cgtgatttcg gcgtgacggt gagcgaggcc
     8941 ggcgggggcg ccgaggcgct gcgcctgctg cccggcgcgg cgcccgacct ggtgctgctc
     9001 gacgtgcaca tgcccgggat ggatggcgtc acgctggcac ggcacctgcg ccagcgccac
     9061 cccggcttgc cggtctacgc gctaaccgcc aacgtgatcg gcagcgagga agcggcgctg
     9121 gcggcggcgg gcgtgcgcgg ggtgctgtac aagccgatcg acgaggcgaa gctgctcgag
     9181 gtgctgcgcc gccatgcgcg ccgcgacgat gggtgggaca tcgaggtgcg cgcgggcatc
     9241 acgcgcgacg aggttgcggc ggaattgcgc cgcctgctgc gcgcggtcga ggcttgcgtt
     9301 gccgcccgcg acctggccgg cggcgttgac gcggcgcacc agttgctggg ggttgcgcgc
     9361 atgttcacgc gcggtgtgct cgccgagcgc tgcctcgcgc tcgaaacggc gctgcgcgac
     9421 aatggcggcg aagcggcgca cggcacgctg catcggctcg cgttgctgct cgggcctgcc
     9481 ggtgcctgag ccttgccggc gcgcgcgctc gcctgcgccg atggcagttc gatcaatcct
     9541 ggtcacaagg agatcgcgat gagtcatggt cgcatgctgc tgtcgtgcgc aatcctgctg
     9601 gtggcgctgc tgggttgcag cgacgaacac ccggggcgcg cgccggcgcg cggcgacgcg
     9661 gcggcggtcg caacgccggt gcctgccgtg acttccgtgg acggtgcgcg catcgccgcg
     9721 gccgatgacg aaccgcacaa ctggctgagc cacggtcgca gctattccga gcagcgtttc
     9781 agtccgctcg ggcacatcga cgcggcgaac gtgggccggc tggggctcgc gtggttcttc
     9841 gacttcccca ccgaccgcgg catggaggcc accccgatcg tggtcgacgg tcgcctgtac
     9901 gtgaccggca cctggagcat ggtctacgcc ttcgacgcga agagcggggc gctgctgtgg
     9961 catcacgacc ccggggtgcc gcggcagtgg gcggtgcacc tgtgctgcga cgcggtcaat
    10021 cgcggtgtcg cggcatgggg cggcaaggtc ttcgtgggca cgctcgacgg gcggctgcag
    10081 gcgctggatg cgaatgacgg caaggtgttg tggtcggtgc agaccacgcc ccgcgacaag
    10141 ccctactcga tcaccggcgc gccgcgcgtg gtgaaggggc tggtgatcat cggcaacggc
    10201 ggctccgaac tgggcgtgcg cggttacgtg agcgcctacg acgccgaaac cggcgacatg
    10261 aaatggcgct tctacacggt gccgggcgat ccggcgcagc ctttcgagtc gcccgcgctg
    10321 gaacgggcgg cggcgacctg gtcgggcggt gagtggtgga agatcggcgg tggcggcacg
    10381 gtgtgggact cgatggccta cgaccccgaa ctcgacctgc tctacgtcgg tgtgggcaac
    10441 ggctcgccgt ggaaccagca gtaccgcagc ccgggtgggg gcgacaacct gtacctgtca
    10501 tcgatcgtgg cgctcgaccc ggacaccggc gcctacgcct ggcactacca gaccacgccc
    10561 ggcgacacct gggactacac cgcgacccag cacctggtcc tcgccgagct gccgatcggc
    10621 ggcacgccgc gcaaggtgat catgcaggcg ccgaagaacg gctttttcta cgtgctcgat
    10681 cgcgcgaccg gggagtttct ctcgggcgat gcctatgcct accagacctg ggccgaggga
    10741 atcgacgcca acggtcgccc gatcccgaac gcgcaggcac gctacgtcgg caaggaggcg
    10801 ctggtgttcc cgtcaccgca cggcgcgcac aactggcacc cgatgagctt cagtccgcgc
    10861 accgggctgg tttacatccc ggtgcaggaa atccccttcg tctacaagca cgacgacaag
    10921 ttcgcgttcg agcccgggct gtggaacacc ggcgtcgacg tcgaggcggc cgggctgccc
    10981 gaggatccgg cggcggctgc cgaagtgctg aaactggtgc agggccacct ggtcgcctgg
    11041 gatccggtgg cgcgcaggga agcctggcgc gtcgagcagg gcgcgccgtt caacggcggc
    11101 gtgctcagca cggcgggtga cctggtgttc cagggcagtg cgcgcggcga gttcgtggcc
    11161 tatcgcgccg acaccggcga gcgcctgtgg gccgcgccgg tgcagaccgg cgtggtggcg
    11221 gcgccgatga catatgccgt cgacggcgag cagtacgtcg cggtcgtggt cggctggggt
    11281 ggcgggctgc cgctcgccgg cgggcgcgtg gcgcagatgg cgggttccgt gaaccgcagc
    11341 cggctgctgg tgtacgcgct ggatggcagg cagcagttgc ccgaaccggt cgcggcggca
    11401 cgggcgctcg cgccgccgcc gctcgccgcg acacccgggc aggtggcgcg cggcaaaacg
    11461 ctgtatgcgg cgcgctgctt cgcctgccat ggcgacggcg cggtgggtgg cggtgtggtg
    11521 cccgatctgc gttatgcctc ggccgcgacg cacgccgagt tcgatgcgat cgtgcgcggg
    11581 gggctgcgcc accagaaggg catggtcggc ttcgcccagg tgttcggcag ggaggacgtg
    11641 gaggctatcc atgcctacgt gatcaagcgc gcgcacgatc tgctcacgga gcaggccgcg
    11701 caggccgcgc gccagggcgg cgacgccggc tgagggccgt tgtcgtggcg ggtggcgcgt
    11761 tgcgccacgc cagccgggtt cagccgaaca gcccgtgcag gaaccagcgc cggctgtggt
    11821 gatcgcggaa cacccagcac agggtgccgt tctgttcgtg tcgcgcgatg aagtagtcgc
    11881 gtttgccctc cgtggcccac cagtcgctct cgatgcgttc cgggccgctc agcagccgca
    11941 ggcgtccgtg ccagcgcagg ccctgcgcgt cccgcgccag cggcagcggc tcgggcaaca
    12001 gccaggacgg gcgctgcgcc gggatggccg tggccccgct ggcggcgcct ccgcccccct
    12061 gcgtgctgcg ccaggcgtgt tccggcaggt gggcgtcggc ggtgccgagg ccatgaacgc
    12121 agccttcccc caggcgtatc gcgaggcggt cgagcagcag gcggccggcc tgctccgcgt
    12181 cctcgtcggc gtcggggaac agcgccgcac tctgctggtg caccggcgcg aagtcgcggc
    12241 acacgagcgc cagcgattcc gccggctcgg ccagccgcag tgactcgaac ttcagccgcg
    12301 tgaggccgat cagggccgcg tggctgttgt gcgcgagcga acagggcagg tcgagcgcga
    12361 gcgtgcggcc gctgatgtcg cgcaggcgcc agtcgaggcg ctggcagaag cactggcggc
    12421 ggtcgaggaa ctgccccagt tcgcgcagca ggcgctgtgc cgggaacagc agcatcgtgc
    12481 tgtggtgcag cggctcgagg aattgcagct cggcgtggaa gaaatcccgc ggctggtgcg
    12541 gcaggcgcgg gtcgggcttc tcgccgccca ggcgctgcag gcgatcgagg aattcgtggc
    12601 cgaaacgctt gccgagcgcg gcgcgcggca gcgcgagcag ggcgccgatg cgggagaagc
    12661 ccatccgctg catgcgcagg cgctgcctgt ccggcacctc gagcagatcc agcggcagct
    12721 cgccgaggcc ggcgcggaag gcggcgaggt cgccgttcgc gcaccacggc gggatatccg
    12781 cgggggtgca ggggcgggcg cgggccagca gggcggcgcc cgccggcgtc ggcgccaggc
    12841 cgtcgaggtg cgcgtggccg cgctcggaca gctcgtcttc cacccgccgc agcaggcgct
    12901 cgaggccacc gaagagacgc aggctgccgc tcacttccag cagcaggctg tcgggcggct
    12961 cgagcgccag cagcgaggtg aagcggtagc cccagtcggc cagcccgagc agcgcggcgg
    13021 cctcgcgttc cttgcgccgt tcgagcgtca gcagcccggg gcacagggcc tgcgcggtgc
    13081 tggccgagat gccggggctc acgccatgct cgccggcgag cgcgtcgtgg cacagcacgc
    13141 ggtgcttttc ggtgacgatg acggggcgtt gcgcgtgcat gtcggcgcgg gtgaagacct
    13201 ccagcggcag cagcgggaag cgcaggcaga gccagagcat ggcggcgcgg gctcagtgca
    13261 gcgccgtccc ggcgggcgtg gtcgtgggtc cggatttatc cggacatggc attcgttcgg
    13321 ccgtgggtcc ggatttatcc ggacatggca tctgtccgga taaagccgga cccacggggg
    13381 cctgatccac ggtggcgggc agcagggcgg ggtcgatgcg cggctgcagc agcgcattgg
    13441 cgcgggcgag cgtgagctgc tggcccgcct ggccgccgcg ctgcttgacg atctcgagcg
    13501 cgaggtgctc ccgcgtgccc cgcagcacga tccgcagcgc ggcaggcgag aggctgtgcg
    13561 ccgcctgtgg cgggcggaac aggaatgcca ggctggcgct gccctgcgcg gcgagctgca
    13621 gccggcgcag ggcgcggtaa tcgccgaggc gcgattcggg cagccaggcc agcaggcagg
    13681 ccgcggcacc ggaacgcagg atctgttccg cgcaccacag ggtgtcgacg tcccgctccg
    13741 cccgcacgac cagcagccgt tccagcgcca cgtcctgtgc cagcaaggcg ggcgcataag
    13801 gcagataggg tgggcagacg aagaacagtc ggcgcgtacc gcggctgcaa tgcgccaatg
    13861 ccggtgccag cagttgcatc tcgccgatgc cacagctgcc gctgagcacc tcggtcagcg
    13921 ccgcgcgcgg ccagccgccc cggtgcagga cccggtcgag ggcggcatgt ccgctgggca
    13981 gggtatcccc ggtcgcggtt accgcgcggg ggcgggaagc ctgccagaca tcggggcggg
    14041 cgagcagtgt ttgcagcaac cggttcatgc tcatgactgt tcaaatatac agtattctgg
    14101 tgattccgtt gcccgttgta aacagcggct actctgcttt cgatgcgtta tgcacatttg
    14161 tggcgcactg ccgcattgcg gaacgcgtat cacgacatcc ataccgaatt cgaaaaatgg
    14221 atttttagta ttcaaaaaca atacgatgcg ttatttttct aatattgaat gtaggtttat
    14281 gggtgggtga gcgtggccga ggcgtgcaac cgcacacttt tatccccggt tttatccaca
    14341 gcatttccag cgccccggcc gtgctgtgct agcatggcca cgccttcgtc ttcccccatt
    14401 ccatagagta ggtagaggga tcaccgccga tgctgcagca actgacgacc atgctggaaa
    14461 tgcaggaccg gatgaaccgc aaggtccatc ccgactggat caaccagcaa ttcgcctggt
    14521 accgggcgct gtggatcgaa tgcggggagc tgatcgagca ctacggctac aagtggtgga
    14581 agcatcagca gccggcgtgg gagcacgtga agctcgagat cgtcgatatc tggcacttcg
    14641 gcatgagcat gcgcttcgat ggcaccagca cgccggcgca gatcgccgcc ggcatgctcg
    14701 aggagttgcg gctcgccccg ccgcagacca tggagttgcg cgaggccgtc gaggccctgg
    14761 cgggccgcgc gctggtgggg cgcgaattcc cggtcggcca tttctggtcc ctgctgcagg
    14821 cggccggcat gacgcccgat gagctgttca ccgcctacgt gggcaagaac gtgctgaatt
    14881 tcttccgcca ggaccacggc tacaaggacg gcagctaccg caagacctgg cagggacgcg
    14941 aggacaacga gcacctggcg gaactgctgg gccagctgga cagccgtagt ccccgctatg
    15001 cggacgagct ctacggcgcc ctggcggcgc gctacgcgca ggctgcagcc gcgaacgcct
    15061 gagctgctgt tatgcacact gcgtaaatgc tgtcaagaaa gtgacattga ccgcaacaaa
    15121 tttgtcattt cggtagaaaa caacattaag aaagtaatgt atctgattgt ttaatatata
    15181 aaaaatgata tttgatcata aaatgaacaa tctgagaggg cctcagccgg catgcggggt
    15241 aggggcgcat actaagaact tatccgcaaa cttatccaca gaaattgtgg acaactggga
    15301 gcccgaaacc ggtgccgggg actgagcaat acctgtcggg atcggcgctg gcgcggcgtc
    15361 tcaacatgcc cagccgcgag ttgttcacgc tgctcgccga gcagggctgg atcgtgcgcc
    15421 agggtgaaca gtggcgcctg acgcgcaagg gcgagttcga gggcggcaga tacctgaaca
    15481 gcgaacgctt cggcacgtat atcggctggc cggagcaggt gctggaacat cgcctgttca
    15541 gcgtgagtcc cgagcagacg ctgctgaatg cggccgcgct gggcctgcgg gtcggcctgt
    15601 cggcgcgcca gaccaacctg ctgctgctcg aactgggctg gatccgcaag ggcgtgaagg
    15661 gctgggagct cactgcccgt ggcaaggcgc tcggcggggt gcaggaggaa tacccggaca
    15721 ccggcgtgcc ttacgtgcgc tggccggcca cgctgcccgg caaccccgtg ctgaacgaga
    15781 acctgcaggt gatccatgcc tacgaacaca tcggggaggc cgcggagcgt gacctgttcg
    15841 gtgatgccga gcgcgagatc cgtgtcgatg gcggggacgg gctgcgcgcg ctcgacgggc
    15901 acgtgctgca cagcaaggcg gagctgatga tctgccactg gctgtacatg tcggagatcg
    15961 cgcacgccta tcaccggcgc ctcccggtgg agggtgacta ccgctgcgat ttctacctgc
    16021 cgtcactgca gctctacatc gagtactggg gcgacgaggc ggcgccgggg caactgacgg
    16081 cgaagctgaa caagaaggcc gtctacgagc gccacggcct caagctgctg gagatcggac
    16141 ccgacgacct cgcccggctc gacgaggtcc tgccgcgcgc gctgttgaag cacggcctcg
    16201 cggtctattg agacggctca ggcggccgtg ctccccagcg cccgcgcgaa gccgtccatc
    16261 gcctccgcca gcgtgaagtc gagcgcggtg atgccgtgcg cgtcatgcgt ggtcaacgcg
    16321 atctcgacgc ggttgtagac gttgaaccat tccgggtgat gatccagctg ctcggcgcgc
    16381 agcgcgctgc gcgtcatgaa cgcgaaggcg gcgctgaagt ccgcgaagcg gtacgcgcac
    16441 cgcagcttgc catcgcgcag gctccagccg ggcagcgcgg cgaggcgcgc agtgatcgtg
    16501 gcatcgtcca gtttttgcgc tcgcatgggc tcactccccg ggcggtgtca ggtggcattg
    16561 cgacgctgca gggtcacgcc gcattccagg tgatgggtat agggaaactg gtcgaacagc
    16621 gcgaagcgtt cgatgtcgtg cgtggccgcg agcgccacca ggttctcgcg cagcgtctgc
    16681 gggttgcacg agatgtagac gatgcgcccg aagcccgcga cctgcgccag cgtgcgcgca
    16741 tcgagccccg cgcgcggcgg atcgaccagc acggtgccgg cttcgaaacg gtcgaggtcg
    16801 aaatcgcgca gccggcggaa gggccgttcg cgcgccagtg cggccgaggc ctccgcgctg
    16861 gagagccgcg cgatgaaggt gttgtcgatg ccgttggccg cgaggttgta ctgcgcggcg
    16921 gcgatcgcga ccttgttgat ctcggtggcc agcacggtgt cgaaacgatc ggccagcgcg
    16981 accgtgaagt tgccgatgcc gcagtagagc tccagcaagc cgccgcgcgc ggcgccacag
    17041 cccgcacgcg cccacgacag catcgcgcgg ttcacctcgg cattgggttg cgtgaaacag
    17101 ccctcgggct ggcgcagcac cagttcgcgc ccgtcgacct gcaggcgttc cgtcacgaac
    17161 gattccccga tcacgatgcg ttgcccgcgc gagcggccga tgagctgcgc atccagcgcc
    17221 aggctcaatt cccgggcggc gtcttcccat gcggcgtcga gcgggcggtg gtagaccagc
    17281 accaccagcg cctcgttggc tagcgtgctc aggaactcga cctggaacag cttgcgcttg
    17341 agtgccggcg agcgcagcac cgccgcgcgc aggcgctgca tcagcgcgtt gatgcgttgc
    17401 gaggcgggcg ggaactcgtc gatgcgcacc ggctcgcgcg gattcgcggg atcgaacatc
    17461 gcgtagaagg cgtcgtcgcc ctcgtgccag aagcggaact cggcgcgctg gcggtagtgc
    17521 cgcgccggcg aggcgtgcac ctcgggcgca ggcgcgccga gctcgcgcat ggcgtcgtgc
    17581 accacgcgca ccttctgctc cagcagcgcg gcgcaggccg gcgcggaata gtcctgctcg
    17641 ctgtatctca cggggtgtct cctggccatg ggccgcgccg gcgccagtaa tcgcgcgcga
    17701 gcgccagcgc ggcgtcggcg tcgttggcgc caccgagcgc cagcagcgcg gccgctgccg
    17761 tgccgatcac agcggcttcc ccgtaggcgt cctcggtgcg gccgttccac agcgcgggca
    17821 gcagcgccac gtccggcacg atggcggcgt cgccgcgttc ggccagctgg cgcgtgaaac
    17881 cgagctcgcg ttgcgcatcg ccctcgagca ggatgcagcg gctgtcggca ttggggcgca
    17941 attcgcattc gccgccctct ccacggaaga tcagcgcgcg cggctgcccg agctgtcgcg
    18001 ccgcgcccgc gtgcagcacg gcataggccg ggtgctgcag gctgtgcatc gtggcgggcg
    18061 cggccagcgg gttcatgtgg cgcacgaagg tgttcaccgg cgagcgcagg ccgaacaggt
    18121 ggcgcaggtt cagcagcgcc tgcaggcccg ggctgaacag cgcgagcggc gcgtacgcga
    18181 aacggcgcgt atccagcgcc tcgcgcacct cctgccagtc gcgcgcggcg gcgatgccga
    18241 gcgccggcag cgcctgggcg gcgaacaggc ggttgcgcgc gtgcggctcg cagccgtgca
    18301 tcagcacgcg gtgcccgtgc gtggccagca gcagcgccga cagcaggtac cacggcggct
    18361 gctggcgctt gcccgcgtag gtcggccagt ccaggtcgac gattatcggg gcctccggca
    18421 gctgtgcgcg ctcgcgcgcg gcctgcacga aaccggcgat ctcctcggcg gtttcctcct
    18481 tcacgcgcag cagcatcagg aaggcgccgg tctgcagcgg gtcggcctcg ccgtcgagga
    18541 tcatcccgag cgccgtgcgt gcctcctcgc gttcgagcga gcgcgcgcca cggcgtccgc
    18601 gcccgagcgt gcgcacgaag ggcgcgaagg gatgttcctc ggggcacgat gatggcggcg
    18661 cggacaggtg catgggcttg ctcacagaca gtgctcgggg cggggcaggc ccgcgatctt
    18721 cgccgccacg cgcgcggggc tgccggggaa cagtccgagc aggtagacgc tgcggccctt
    18781 gtcgggaccg agcgtgcgtc gcgtcaggct gaccaatgcg cgcatggcgg gcgcgtgctc
    18841 gtgctcgcga tagtacgctt gcagcatgtg caggatctcg cggtgcgcgg gcgtcagcgt
    18901 gaggccttcc tcgcgcgcga gcgcggtggc cacctgctcg ttccagtcgg cccgatccag
    18961 caggaaaccg tccgcatcgc gcggcggcag cgctgcgttt gctttcatct cagaaccagg
    19021 acacggtgcg cgggtgtgcg accgcgaggt cg
//
LOCUS       DAORUQ010000038        18993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2524_length_18993_cov_6.539339, whole genome shotgun sequence.
ACCESSION   DAORUQ010000038 DAORUQ010000000
VERSION     DAORUQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18993
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2524_length_18993_cov_6.539339"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLW13_05325"
     CDS             complement(<1..980)
                     /locus_tag="PLW13_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69825.1"
                     /translation="MTDRFTEDAGESASISISYRVTFETQPAPAQLQTRSANPVPADP
                     RVELERILAQEFDEAYYREQFPEGYTSVQLPLVHYLSIGWREGKNPNAWFDTAYYLQH
                     NPDVRFSNVHPFVHYLQFGIREKRNPSAIGLQARKPLAKDVVPAVVPNERRGVDRMAE
                     VKDAIGESFDAAWYARKYPEVSLSMRAPLDHFVETGWREGKDPAPWFSTRYYLASNVD
                     VAQADINPFFHFLVQGKREGRLASHPGGERAEVLRNLQTMPARIASWTYGKRIVVDAG
                     QLLKCKLAEGLEHPDFVISISHDVYFSNVGGIQTCIGREQGVFNQERVHYL"
     gene            complement(1023..2030)
                     /locus_tag="PLW13_05330"
     CDS             complement(1023..2030)
                     /locus_tag="PLW13_05330"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQY69826.1"
                     /translation="MESHRDALATNKSISRYGLIEHFPSDLIGNAVKAYGEWAWLETA
                     FIAQLLDELVQSNRCAAPVWYDVGAFIGTFSLGLHALTGGFRGGSGFSGVAIEGNVGS
                     ARVLENNVAALATGRIEVVTEVLGVDGHKVRATSDAVNRGATSFGSHADDADGEFEER
                     ICVPLWRIRNSFGPYGLLKLDVEGMEREVIQSDQEWIGQNKPVVYAECNESPNSLALL
                     DLLLWLGYDVYFFSFPAFNPGNFLGVPGDLLFNGAHEAALIGVPGRVCSGNDFERHGC
                     VFRKLESRADLVKALWLTPRFVSRQFRDLGVSELRAVAERLSKGLTLDGFAHALGTDA
                     G"
     gene            complement(2391..3500)
                     /locus_tag="PLW13_05335"
     CDS             complement(2391..3500)
                     /locus_tag="PLW13_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY69827.1"
                     /translation="MRVLHFYRTFYPASYGGIEQVIYQICHGCEPRGIFAEVLTLGSR
                     ERVGDTVFDTIPVHYAKTQFRIGGAEISASAIGRFRDLAAKADLLHYHFPWPMADVAH
                     LLASAGKPSVLTYHSDIVRQIRLYRIYRPLMNVFLGRIDRIVATSPNYLQTSPVLAQY
                     RDKTSVIPIGLDEANYPGPDAARLAYWRARLPAPFFLFVGMLRYYKGLHILLDALKGS
                     AANVVIVGAGPIEHELKDHARREGLGNVTFLGAIPDEDKVALLTLCYGVVFPSHLRAE
                     AFGVSLLEGAMFGKPMISSEIGTGTSHVNVGGVTGLVVPPGDSAALRAALARLLDEPQ
                     LARQMGRNARARYLELFRAEQMCASYAELYRGVLR"
     gene            3643..4488
                     /locus_tag="PLW13_05340"
     CDS             3643..4488
                     /locus_tag="PLW13_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY69828.1"
                     /translation="MRPISLDISIVTYKTDLLGLKIALISLCQTIERARERLARQLLV
                     SLFIVENAPRHPTPPPSRLAASRYLRAFDAIHHVQSPTNVGFGRGHNLAIFDARGATA
                     DYHLVLNPDVELDPEALRLCIEYLEATPEAVAVVPNAVDGSNEPCYLAKAQPSVVVLA
                     SRAYLPGIRLPWLRKRLERYELRPAADRGEIIRDVPNGSGCFFFARARALREVGGFAP
                     QYFLYFEDFDLSNRLRRLGRMDYLPQVRLIHHGGNVGRKGLHHLVYFSRSAIRFFNRW
                     GWRFC"
     gene            complement(4495..4908)
                     /locus_tag="PLW13_05345"
     CDS             complement(4495..4908)
                     /locus_tag="PLW13_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69829.1"
                     /translation="MLRFIGFSIFALFLLGGCAAQPQTPASAEEAVKARATAQQQARL
                     ENNFQLAYTFTSPGYRETHPYKFYLGRMGTVIKRRGFEVKSVSCEGDVCSVLVNLEYS
                     YAGLAAGASKDAVMKRELTEKWIRADGEWWLVPDR"
     gene            complement(4972..7113)
                     /gene="gspD"
                     /locus_tag="PLW13_05350"
     CDS             complement(4972..7113)
                     /gene="gspD"
                     /locus_tag="PLW13_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HQY69830.1"
                     /translation="MKSGSYTTVRRRPVGWLHTTLLVVSFAVLGACSSTTAQKPQAPG
                     RIFAGVKAPPAAATDDAKPVEAPRDRTVYEQPGTQQMVRDTAGPAPIIRLGEGKVEVN
                     FRDAPIAEVVKVILGDNLRVPYTIEGEIKGNITLSSSSPVSEDALIALLESLLANHGI
                     VMVRSEDGIYRLGPPEALQSTTPVRVGKAAAGTSGFDTRIVPLQYISVAEAKKVLKPL
                     GAEKNILLADPVRNLLMLAAPAPQMNNIIRTLASFDIDVMKGMSFGIYEIFNTDATAV
                     VDRFNELLGGEELSPLSGVVKLVALEEINNVLVITSRAHYLETARTWIERLDQFTVRD
                     GESASRRLFVYNVNNGEATALASMLTQMFGGTAGTVQQKQKARKTAGKVAPGESRAAV
                     GQAETPEPQQLGSMNDATADNDDPKNANVPRVVADESNNTLLVMATPQVWREIENALR
                     KLDVMPAQVLVEVSIWEVALNDALEYGVEWYFQHSNSSVNGVGTLDFPPAGVNSVLGG
                     FSYLLSDNGGDWRAVINAIDEQSQIQVLSSPSVLVLDNQTADIVVGDQQPVLSGTSAT
                     DGGTVTENIVYKDTGVKLSVTPRVNDSGLVVMDISQEVTDVGSIDAATGQRSFLQRSI
                     NSTVAIQSGDTIILGGLIQSNKTTGSSGVPVLHRLPVVGSLFGTKSDNDRRTELLVTI
                     SPRAIVQYNDFVKVGEEFREKMSGVTAAFGL"
     gene            complement(7180..7692)
                     /locus_tag="PLW13_05355"
     CDS             complement(7180..7692)
                     /locus_tag="PLW13_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69831.1"
                     /translation="MSMTLQARTMMTTRVLLGTIGACLGLAALLWLLAGPSAPDSDVE
                     PLEVRIPDAAGIAQGPGEDYPMMLEQPLFWAERAPSLAADGDSASAGTAAGPDGLVYL
                     GVLVNEPRRQVLLRDGDSVHVLREGETIRGLEVRRITAEGVLLGNATSEVKLPVPVDR
                     SEFIEIRRME"
     gene            complement(7689..8279)
                     /gene="gspM"
                     /locus_tag="PLW13_05360"
     CDS             complement(7689..8279)
                     /gene="gspM"
                     /locus_tag="PLW13_05360"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HQY69832.1"
                     /translation="MGNLTPDYRRLAAWGVVLALVLVLLSAVLGPVVARHREYDAEIA
                     RDRQVLDRLTRLEVSRDRIREAARRYREEDLASLVYSQGMPPAQIALDVQKRVTQILD
                     KSGAEVKTVASFNLPAEDFQGSGIKVTFTGSLEAVASALHEIESGRPLLVVEEMDIKP
                     LMQGRMARSAKEQPAPQSVQVQLAVVAYTPLPSKQG"
     gene            complement(8279..9391)
                     /locus_tag="PLW13_05365"
     CDS             complement(8279..9391)
                     /locus_tag="PLW13_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQY69833.1"
                     /translation="MDFSSADKWTLFGVDVLKILRAWWGELSRSVPDRVKQLFLLQPA
                     RVVIAMRGEQCVLLEDTQERSGELAVLERRTLDEHAAGLLAAQLDDIERRGGAGLVID
                     LLLEPSALLRRRLELPIQLESSLRQGVAYQLNRLTPFSEADLYFDVVVRERDTASRKL
                     QVELVAAPRSRVDPLLHEMTRLCGRQIDRLTTADLGPAANLLGGSHTRFRPNRNFWLL
                     LALLGCLGVLVVTPLMAKRSAMLQQKAAIRELQQSVGELAREKNTLETDGRSLAYIVE
                     RRGQSPRMVTLVDELSYLVPKSIYLTQLAVSKGRVRLTGQGAGVVGLIDILNGAKFLD
                     EAKFESTVSRNPRTGLDQFSVGAKLVGTQAAAVQEK"
     gene            complement(9410..10366)
                     /locus_tag="PLW13_05370"
     CDS             complement(9410..10366)
                     /locus_tag="PLW13_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HQY69834.1"
                     /translation="MSALRTLRNGRGAQRGAVLVLVLWILTLLTFLLGAFSNEIRVEN
                     RIVGDMVGRIRLRAGATAALNYLALVGQASPDTLATLEGVPLSLALGNARVAYRFVPE
                     EAYVSLNAAPPEMLRALIGNYPDGVSDVDALVNALVDWRDGDEIVTENGAEADEYASA
                     GLAYGPTNAPLLSVDELGLVLGFDQDLVDWLRPYVSVASMSAGIDPRFADPELVMMLD
                     TEGRFGEQDLQAMQADPAVAEAMALDSSFFSASRSGAYRLLVQGSGAMGLKRRQAIEV
                     VAAFEGGGAPPVAGLEAVVTGPVPAPYRIIQWNEFPSLDVDG"
     gene            complement(10363..10968)
                     /locus_tag="PLW13_05375"
     CDS             complement(10363..10968)
                     /locus_tag="PLW13_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQY69835.1"
                     /translation="MNACPARARGFTLVEVLIVLVLTSLITLTLFSAFRTGIKSWRTA
                     EDHLQRVEGARQLLNVVHRHLFQVSPVVLMFPDRTEFAFSGTASSLRYVAPLSMSAGG
                     ELYVVELFSDEGGSPGVWGRFVPYSPQGMDEASTDNEASLISEDMVISFQYYGATDAS
                     GEEIGWGDSWAGRPEFPQLVRISFTGGRHAMPPVVMRVGAG"
     gene            complement(10965..11378)
                     /gene="gspI"
                     /locus_tag="PLW13_05380"
     CDS             complement(10965..11378)
                     /gene="gspI"
                     /locus_tag="PLW13_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HQY69836.1"
                     /translation="MPRIPRRLAQRQTGFTLLEVLVAFVLAAIGIVALIQAFAGGLRN
                     LGKMDEFVMAAMVAESRLAEVGILYPVAVGEMEGVEEGDKYHWRISVSPFQDPDGLAV
                     MPEQTPALYLVEVDVNWDSGRQPGSFRLQSLRVGP"
     gene            complement(11387..11824)
                     /locus_tag="PLW13_05385"
     CDS             complement(11387..11824)
                     /locus_tag="PLW13_05385"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY69837.1"
                     /translation="MRGFTLIELLVVMMILALAATTVGPLLSRGVGSQLQSSVRDLAT
                     GLRWARSEAVSERRSVALWVDTADKSYVVDTRTRRYRLPEEAKVLVNTAQREVRGDVA
                     GIRFYPDGSSSGGSIELGAEGVVFEVSIDWLTGAVDVARLGVE"
     gene            complement(11847..12281)
                     /gene="gspG"
                     /locus_tag="PLW13_05390"
     CDS             complement(11847..12281)
                     /gene="gspG"
                     /locus_tag="PLW13_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQY69838.1"
                     /translation="MHRHSSRFARAAGFTLMELLVALVILGMLAGIVGPRIMKYVGGA
                     KSDTAKVQIEDLVTSLHMYQLEVGNYPTQEAGLQALVEQPQGVVGWNGPYLSKARVPK
                     DPWGRDYQYRHPGEHGEFDVFSYGADNNPGGEKDDADIVSWE"
     gene            complement(12297..13520)
                     /locus_tag="PLW13_05395"
     CDS             complement(12297..13520)
                     /locus_tag="PLW13_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQY69839.1"
                     /translation="MAHFRYRAISAEGKVTRGEIEHDSVERAAAQLARQGLTPVSIEP
                     QAAGGSRGGTDALARLFSRRRGGQNAIGEFTRELAIMLSSSVPLDRSLAVLQGLATDA
                     RLKEIIGAISKRVRTGGTLADAFREHPEFSDFFVNMIRAGESSGALESTLTRLAEHME
                     RTKALKSTVSSALMYPAILLLVASGSLIILLGFVVPKFADMFTEMGGTLPLPTRIVMA
                     AGGWVASYWWLLVLAIGGAVALVMNLLAKPEFRERLDRKLLSIPLTGDLVRKVETARL
                     AHTLGTLLQGGVVLVNALSIALATVGNRTLRADLEQGVAALKEGKLLSSALIEHSHFP
                     PLAMHMIQVGEETGQLEPILLKVAAIYDEEVSGAVKRLLALLEPALILGLGVLIAGII
                     VSIMVGIMSVNELVY"
     gene            complement(13539..15242)
                     /gene="gspE"
                     /locus_tag="PLW13_05400"
     CDS             complement(13539..15242)
                     /gene="gspE"
                     /locus_tag="PLW13_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HQY69840.1"
                     /translation="MNGTGPASGLGKLLVARGKLAEPDLQRAERVLAISGGTLGQALV
                     RLGLVAEKEVADAVSELTGCALIGPDDFPEAPVAEERISGEFLQQFNVLPLADDGECL
                     RVAMADPSDDYVLRALEIATERRIEPLLALASDLEQAHERLYGGGRSAMDQIVDGVQS
                     DAVLQEDVEDVERLKDMASDAPVIRVVNLVFSRALEARASDIHIEPYEHELKIRYRID
                     GVLQDVESPPARMAPAIISRVKILSRLNIAERRLPQDGRIKLKISGKEVDLRVSTVPT
                     LHGESVVMRILDKQTLNLNLEDIGLSDKVLSDLKSVLERPNGIFLVTGPTGSGKTTTL
                     YAVLSRMNTPERKIMTVEDPVEYQIAGVNQIQAAPRIGLDFAHALRSIVRQDPDVIMI
                     GEMRDRETAGIAIQSALTGHIVFSTLHTNDSASGVTRLLDMGLEDYLLTSTVNAIMAQ
                     RLVRVLCPACRRESALTDEEVQHMGISRLVERAGSRRVWRATGCEACARTGYRGRMGV
                     HELLLVDDSIRHLILEHASAEVIMKAARANGMVTMYEDGVLKAFAGHTSIDEVLRVTH
                     Q"
     gene            complement(15253..16317)
                     /locus_tag="PLW13_05405"
     CDS             complement(15253..16317)
                     /locus_tag="PLW13_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY69841.1"
                     /translation="MSAPERTAGSASGRETCVDVIIPVYRGLGETRACIESVLASRCR
                     TAAQIIVIDDASPEPELRRYLRGLEGVTVLENAANLGFVATANRGMALHPARDVVLLN
                     SDTVVANDWLDRLRACAWREPATASVTPFSNNATICSYPVFCEDNALPQGMSVAELDA
                     LFREVNRGEAVPVPTAVGFCMYIRRDCLAQVGLFDVALFGRGYGEENEFCLRAARQGW
                     RHLLCADTFVYHAGGVSFADTQSEQKQAAMKTLTALYPDYEALIQRFVAADPPAPYRR
                     AVDVARQRRDEGEGELVTPARRNFARPGGPDALAGEADGAGDGRYDAPRRTRVWSALG
                     SLLPPQWRIRLKHWLQQRDG"
     gene            complement(16304..17560)
                     /locus_tag="PLW13_05410"
     CDS             complement(16304..17560)
                     /locus_tag="PLW13_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69842.1"
                     /translation="MSSDVAIRLDGIAKRYRIFAHPFDRLLYQLFGWRAQRCERFDAL
                     QPMSLDVRRGETLALIGRNGAGKSTLLQIICGTLSPSAGTVTVNGRIAALLELGAGFN
                     PEFTGRENVYMNGAIYGLGRAEIDDAMPSILAFAEIGDFIDQPVKTYSSGMFVRLAFA
                     VIAHVNADILIIDEALAVGDAYFVQKCIRFIRQFKEHGTLVFVSHDVHSVITLCDKAL
                     WLDRGCIARLGPAKEVCEAYLGSLYPEQQARNDAQESAGAVEHPAGQFGTGQARVEQV
                     ELLGQDGTPLKLLMAAQRVRLRLSGRAFTDIDAPIAGFFVKNRLGEYLLGGNTLKQLP
                     DWRFVRAGSRFVVEFAFRMPLFATGDYIVGVAIAAGTQERHVQHHWVHDALHFTAEAD
                     PAMMGWIAIEDLACRVGEPEPADERA"
     gene            complement(17550..18329)
                     /locus_tag="PLW13_05415"
     CDS             complement(17550..18329)
                     /locus_tag="PLW13_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69843.1"
                     /translation="MLKQHVFLIYQMSRREVLGRYRGSMMGLLWSFASPLVMLAVYTF
                     VFGTVFRVRWQTGDGDTASFALNLFAGVLLHSFLAECLNRSPALIAENTGYVKKVVFP
                     LWIIPVVLVFAALFHLLVGLLVLLVAAGVVQQQVHAGVLLLPLLLLPFALLALGLSWF
                     IAALGVYLRDLAQVLPVVSTMLLFLAPVLYPLEALPAGLREHLYLNPLTYVIGELRAL
                     VLGGAMPDWQTLARFTAVALLVALLGWQVFRKAQPGFADVL"
     gene            complement(18373..>18993)
                     /locus_tag="PLW13_05420"
     CDS             complement(18373..>18993)
                     /locus_tag="PLW13_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQY69844.1"
                     /translation="RFQIAMQSDQRLMKAALDAAVAGEREPDFEKLAREKYVANPEQF
                     TQPARVRASHILVSVGAERGRDEALKRAQDARRRAVEGKTPFEELALELSDDPSVKTN
                     GGDLGYFERGKMVKPFDEAAFALTEPGQISEVVETQFGFHVIKLGERKPEHKLEYDEV
                     KAELISREREKFQAALRKQKVEETRSLEGIEVNQAAVSALVQAAPR"
BASE COUNT         3207 a         6575 c         6088 g         3123 t
ORIGIN      
        1 aggtaatgca cccgctcctg gttgaaaaca ccctgctccc tgcctatgca cgtctgtatg
       61 ccgccaacgt tggagaagta gacgtcgtga ctgatcgaaa tgacgaagtc cggatgctcg
      121 agcccttcgg cgagcttgca cttcagcaac tgtcccgcgt cgacgacgat tcgctttccg
      181 tacgtccacg acgcgatccg cgcgggcatt gtctgcaggt tgcgcagtac ctctgcccgt
      241 tcaccaccgg ggtgcgaggc cagccgaccc tcgcgcttgc cttgaacgag gaagtggaaa
      301 aacgggttga tgtcagcctg cgcaacgtcg acgttgctcg ccagataata acgagtactg
      361 aaccagggtg ccggatcctt gccttcgcgc catcccgtct cgacgaaatg atcgagcggt
      421 gcgcgcatgg atagcgacac ctcgggatac ttccgtgcgt accatgctgc gtcgaagctt
      481 tcgccaatgg cgtcctttac ttcggccatc cggtcgacgc cccgccgctc gttcggtaca
      541 accgcgggca cgacatcctt cgctaaaggc ttccgcgcct gcagccctat ggcacttgga
      601 ttgcgctttt ccctgatacc gaattgcagg tagtgaacaa atggatgcac gttggagaag
      661 cggacgtccg gattgtgctg caggtaatag gcggtatcga accaggcatt cgggttcttg
      721 ccctctctcc agccgatgct caggtaatga accagcggca gctgaaccga ggtgtaacct
      781 tcgggaaact gctcgcggta gtacgcctcg tcgaactcct gcgcgaggat tcgctccagt
      841 tcgacgcgcg gatcggccgg aaccggattc gcggatcggg tctgcagttg tgcgggtgcc
      901 ggctgagttt cgaacgtgac acgataggaa atggaaatac tcgccgactc gccggcatcc
      961 tccgtaaatc tgtccgtcat ccggtgttgt cctcaaacat ccttcgcgtg ccaggcgttc
     1021 gttcaacctg catctgtacc cagggcgtga gcgaatccgt ccaacgtaag ccccttgctc
     1081 aatcgctccg ccaccgcacg cagttcggaa actccgaggt cgcgaaactg gcggctgacg
     1141 aatcgcggcg tgagccacag cgccttcacc agatccgccc gggactccag cttgcggaac
     1201 acgcacccgt gacgctcgaa gtcgttgccg ctacacacac ggcccggcac gccgatcaat
     1261 gccgcttcgt gcgctccatt gaacagaagg tcaccgggta caccgagaaa atttccagga
     1321 ttgaacgccg gaaacgaaaa gaagtacacg tcatatccca gccacagcag caggtcgagc
     1381 agagccaagg aattcgggct ctcgttgcat tccgcataaa cgaccggctt gttttgccca
     1441 atccactcct ggtcactctg aatgacctcg cgctccattc cctcgacatc gagcttcagc
     1501 agcccgtacg ggccgaatga attgcggatg cgccacaagg gaacgcatat gcgttcttca
     1561 aactcgccgt cggcatcatc ggcatgcgag ccgaagcttg tagcgccgcg attgactgca
     1621 tcgctcgttg ccctcacctt gtggccatca acaccgagca cttcggtgac gacctcaatg
     1681 cggccagtcg ccaacgccgc gacgttgttt tcgagtacac gtgcgctgcc gacgttcccc
     1741 tcgatggcca caccagagaa tccgctgcca ccgcgaaatc cgccggtcag tgcgtgcaaa
     1801 ccaagactga aagttccgat gaaggccccg acgtcgtacc agaccggcgc tgcgcacctg
     1861 ttcgactgaa ccagttcatc gagcagctgc gcaatgaagg cagtctccag ccatgcccac
     1921 tcaccgtagg ctttcaccgc gttcccgatc aggtccgaag gaaaatgctc gatgagccca
     1981 taccgcgaaa tggatttgtt tgtcgctaag gcatcacgat ggctttccac gcaagagctc
     2041 cagacagcta accgggtcga cgcacttgcg cgtcaggcag gctctgtaac gcgtgtcccc
     2101 gaattgatca ggccggactg tgactggagc ctagcttcaa aagcacacat ttcaaaggcg
     2161 cgggattata agcactacgc agtgcatatc tatggcccat ttgagctaca agccactggt
     2221 actgcgtgct gcaagatgtc gaggaaccgg ccgacgcccg cgccaattat gcatgccgcg
     2281 ttatgcaaga tgcgtgacgc actcaacatt gcgacgcctg aacagagaca gcgcggcgtc
     2341 aaggtcgctt gcaacgacaa attcgcccgg gaagtcgcgc aaccggcaag ctagcgcagc
     2401 acgccgcgat atagctccgc gtaggacgca cacatctgct cggcgcggaa cagctcgaga
     2461 tagcgcgccc tggcgttgcg tcccatctgc cgcgcgagct gcggttcgtc gagcaggcgc
     2521 gccagcgccg cgcgcagggc agccgaatcg ccgggcggca cgacgagccc ggtgaccccg
     2581 cccacgttga cgtggctcgt gccggtgccg atctcgctcg agatcatcgg cttgccgaac
     2641 atcgcgccct cgagcagcga gacgccaaag gcctccgcgc gcaggtgcga ggggaagacc
     2701 acgccgtagc acagcgtcag cagcgccacc ttgtcctcgt cgggtatcgc gccgaggaat
     2761 gtcacgttgc cgagaccttc cctgcgcgcg tggtccttga gctcgtgctc tatcgggccg
     2821 gcgcccacga tcaccacgtt cgcggcggac cccttcagcg catcgaggag gatgtgcagc
     2881 cccttgtagt agcgcagcat gcccacgaac aggaagaacg gcgcgggcag gcgggcgcgc
     2941 cagtacgcca ggcgcgcggc atcgggcccg gggtagtttg cctcgtcgag cccgatggga
     3001 atcacggagg tcttgtcgcg gtactgcgcg agcacggggc tggtctgcag gtaattgggc
     3061 gaggtcgcca cgatccggtc gatccgcccg agaaacacat tcatcagcgg acggtagatc
     3121 cggtacaacc ggatctggcg cacgatgtcg gaatgatagg tgagcaccga gggcttgcct
     3181 gcgctcgcca gcaggtgcgc gacgtccgcc atcggccagg ggaaatggta gtgcagcagg
     3241 tccgccttcg cggcgagatc acggaagcgg ccgatggccg aggccgatat ctccgcgccg
     3301 ccgatgcgga actgtgtctt cgcgtaatgc accgggatgg tgtcgaacac ggtgtcgccg
     3361 acgcgctcgc gcgagcccag cgtcagcact tcggcaaaga tgccgcgcgg ctcgcagccg
     3421 tggcagatct ggtagatgac ctgctcgatg ccgccgtagg aggccgggta gaatgttcgg
     3481 taaaaatgca ggacacgcat cgggcaggcg gcaaatggac ctgcctgtca gaccctgacg
     3541 gtgacacgca ttggtcaatg ggtggaaaat gcaggccgca atttagcaag gatccccggc
     3601 ccgcggtagc ccggtcgaca cgccggcacc gcgggagcat gcatgcgccc gatcagcctc
     3661 gatatatcga tcgtgaccta caagacggat cttctcggcc tgaagatcgc gttgatatcg
     3721 ctgtgccaga cgatcgagcg tgcgcgcgag cgcttggcgc ggcagttgct ggtctcgctg
     3781 ttcatcgtcg agaacgcgcc gcgccatccg acgcctcccc cctcgcgcct ggccgcgagc
     3841 cggtacctgc gcgcattcga cgccatccac cacgtccagt cgcccacgaa cgtgggcttc
     3901 ggccgcggcc acaacctggc catattcgac gcacgcggcg ccacggcgga ctaccacctg
     3961 gtcctcaacc cggacgtcga actggacccc gaagcgctgc ggctgtgcat cgaatacctg
     4021 gaggcaaccc cggaggccgt cgcggtcgtg ccgaacgccg tggatggcag caacgagccc
     4081 tgctacctgg caaaggcaca accatcggtc gtggtactgg catcgcgcgc ctaccttccc
     4141 ggcatccggc tgccgtggct gcgcaagcga ctggagcgat acgaactgcg ccccgcggcg
     4201 gaccgcggcg agatcatccg cgacgtgccc aacggcagcg gctgcttctt cttcgccagg
     4261 gcgcgcgcat tgcgcgaggt gggcggcttt gcgccgcagt acttcctgta tttcgaggac
     4321 ttcgacctgt cgaaccggct gcgccggctg gggcgcatgg actacctgcc ccaggtccgg
     4381 ctgatccatc acggcggcaa cgtcgggcgc aagggcctgc accacctggt gtacttctcg
     4441 cgctcggcaa tccgtttctt caaccgctgg ggctggcggt tctgctagca gccctcagcg
     4501 gtcgggcacc agccaccact cgccgtcggc gcggatccat ttctcggtca gttcgcgctt
     4561 catcaccgcg tccttgctgg cacccgcggc aagcccggcg taactgtact ccaggttgac
     4621 gagcacgctg cacacatcac cctcgcagga aacgctcttc acctcgaagc cgcggcgctt
     4681 gatcacggtg cccatcctgc cgagatagaa cttgtaggga tgcgtttcgc ggtagcccgg
     4741 cgaggtgaac gtgtaggcca gctggaaatt gttctcgagc cgcgcctgct gctgcgccgt
     4801 ggcacgcgcc tttacggcct cctccgccga tgccggcgtt tgcggctggg ccgcgcagcc
     4861 cccgagcagg aacaacgcga agatggaaaa accgatgaac ctcaacactt cttcaaactc
     4921 cctcgatatc aatgtgatca cgccggccaa gggacgaatg gcatccgccg ctcacaggcc
     4981 gaaggctgcc gttaccccgc tcatcttctc gcggaactcc tcgccgacct tgacgaagtc
     5041 gttgtactgc acgatcgcgc gcgggctgat cgtcaccagc agttccgtgc gccggtcgtt
     5101 gtccgacttg gtgccgaaca ggctgccgac gaccggcagc ctgtgcagca ccggaacgcc
     5161 cgaactgccg gtggtcttgt tgctctggat gaggccgccg agaatgatcg tatcgccact
     5221 ctggatcgcc acggtgctgt tgatcgagcg ctgcaggaag gagcgctgcc cggtcgccgc
     5281 atcgatgctg ccgacgtcgg tgacttcctg cgagatgtcc atcaccacca ggccgctgtc
     5341 gttgacccgc ggcgtcacgc tgagcttcac gccggtgtcc ttgtagacga tgttctcggt
     5401 caccgtgccg ccgtcggtgg cgctggtgcc cgagagcacc ggctgctggt cgccgaccac
     5461 gatgtcggcg gtctggttgt cgagcaccag caccgagggc gaggagagca cctggatctg
     5521 cgactgctcg tcgatcgcgt tgatcaccgc gcgccagtcg ccaccgttgt cggacagcag
     5581 ataggaaaag cccccgagca cggaattgac ccccgcgggc ggaaaatcga gcgtcccgac
     5641 cccgttcacg ctgctgttgc tgtgctggaa gtaccactcg acgccgtact ccagcgcgtc
     5701 gttgagcgcg acctcccaga tcgagacctc gaccagcacc tgcgcgggca tcacgtcgag
     5761 cttgcgcagc gcgttctcga tttcgcgcca cacctgaggc gtggccatga ccagcagcgt
     5821 gttgttgctc tcgtcggcga ccacgcgcgg cacattggcg ttcttcgggt cgtcgttgtc
     5881 ggccgtggca tcgttcatcg agcccagctg ctgcggctcg ggcgtctccg cctggccgac
     5941 cgcggcgcgg gattcgcccg gcgccacctt gcccgcggtc ttgcgcgcct tctgcttctg
     6001 ctgcacggtg ccggccgtgc cgccgaacat ctgcgtcagc atcgatgcca gcgccgtcgc
     6061 ctcgccattg ttgacgttgt agacgaacag cctgcggctg gcgctctcgc catcgcgcac
     6121 ggtgaactgg tcgaggcgct cgatccaggt cctggcggtc tcgaggtagt gcgcgcgcga
     6181 ggtgatcacg agaacgttgt tgatctcctc cagcgccacc agcttgacca cgcccgacag
     6241 cgggctgagc tcttcgccgc cgagcagttc gttgaaacgg tccacgaccg cggtggcatc
     6301 ggtgttgaat atctcgtaga tgccgaagga catccccttc atcacgtcga tgtcgaagct
     6361 cgcgagcgtg cggatgatgt tgttcatctg cggagccggt gccgcgagca tcagcaggtt
     6421 gcgcaccggg tcggccagca ggatgttctt ttccgcgccc agcggcttga ggaccttctt
     6481 cgcctccgcc accgagatgt attgcagcgg cacgatgcgc gtgtcgaagc cgctggtgcc
     6541 ggcggctgcc ttgcccaccc gcaccggcgt cgtgctctgc agcgcctcgg gcggtcccag
     6601 ccggtagatg ccgtcctcgg agcgcaccat cacgatgccg tggttcgcga gcagcgattc
     6661 gagcagcgcg atcagcgcat cctcggaaac gggactgctg gagctcagcg tgatattgcc
     6721 cttgatctcg ccctcgatgg tgtagggcac gcgcaggttg tcgcccagga tcaccttcac
     6781 cacctcggcg atcggcgcgt cgcggaaatt cacctcgacc ttgccttcgc ccaggcggat
     6841 gatcggcgcc ggccccgcgg tgtcgcgcac catctgctgg gtgcccggct gctcgtacac
     6901 ggtgcgatca cgcggagctt ccaccggttt ggcgtcgtcc gtggctgcgg cgggcggcgc
     6961 cttgacgccg gcaaatatcc gccccggtgc ctgcggtttc tgcgcggtcg tcgagctgca
     7021 cgcgccgagc accgcgaacg acaccaccag gagtgtcgtg tgcagccagc cgacggggcg
     7081 gcgccgcacc gtggtatacg atcctgattt catcatctgt ccagacaaag tcctgttgcg
     7141 ctctcgcaga gcatagccca cgtcgaccgg aggcacggat cactccatgc gccggatctc
     7201 gatgaattcg cttcgatcga cggggacggg gagtttcacc tcggaggtgg cattgccgag
     7261 cagcacgccc tcggcggtga tgcgcctgac ctcgaggccg cggattgtct caccttcgcg
     7321 cagcacgtgc acgctgtcgc cgtcgcgcag cagcacctgt cgccggggct cgttgacgag
     7381 cacgccgagg tagaccagcc catcgggccc ggcagcggtc ccggccgatg cactgtcgcc
     7441 gtccgccgcc agcgagggcg cgcgctcggc ccagaacaac ggctgctcca gcatcatcgg
     7501 gtagtcctcg cccgggccct gcgcgatgcc ggcggcatcg gggatcctga cctcgagcgg
     7561 ctcgacatcg ctgtcgggcg ccgacgggcc ggccaacagc cacaacagcg ccgcaaggcc
     7621 caggcaggcg ccaatagtcc ccagcagcac ccgcgtcgtc atcatcgtgc gcgcctgcag
     7681 cgtcatgctc atccctgttt gctcggcagg ggcgtgtagg ccaccacggc cagttgcacc
     7741 tgcacggatt gcggcgccgg ctgctccttg gcgctgcgcg ccatgcggcc ctgcatcagc
     7801 ggcttgatgt ccatctcctc gaccaccagc agcgggcgcc cggactcgat ctcgtgcagc
     7861 gcgctcgcga ccgcttccag cgatccggtg aacgtcacct tgatccccga gccctggaaa
     7921 tcctctgcgg gcaggttgaa ggaggcgacc gtcttcacct ccgcgccgct cttgtccagg
     7981 atctgcgtca cccgcttctg cacgtccagc gcgatctgcg ccggcggcat cccctgggag
     8041 tagaccagcg aggcgaggtc ctcttcgcgg tagcgcctcg ccgcctcgcg gatgcgatcg
     8101 cgcgagacct cgagcctggt gagccgatcg agcacctggc ggtcgcgggc aatctccgca
     8161 tcgtattcgc ggtgccgcgc cacgaccggc ccgagcaccg cgctcagcag caccagcacc
     8221 agtgccagca ccacccccca ggcagcgagc ctgcgatagt ccggcgtcag gttgcccatc
     8281 acttttcctg cactgcggcg gcttgggttc cgaccagttt cgcgccaacg ctgaactggt
     8341 cgagcccggt acgcgggttg cggctcacgg tcgattcgaa cttggcttcg tccaggaatt
     8401 tcgccccgtt gaggatatcg atcaggccga ccacgccggc gccctgcccg gtcaggcgga
     8461 cccggccctt ggaaaccgcc agctgcgtga gatagatgct cttgggcacc aggtaactca
     8521 gctcgtcgac gagcgtgacc atgcgcggcg actggccgcg gcgctcgacg atataggcga
     8581 gcgaacggcc gtcggtctcg agcgtattct tctcgcgcgc gagctcgccc acgctctgtt
     8641 gcagctcgcg tatggcggcc ttctgctgga gcatcgcgga gcgcttggcc atgagcggcg
     8701 taaccaccag cacgccgagg cagcccagta gcgccagcag caaccagaag ttgcggttgg
     8761 gacgaaaccg ggtgtggctg ccgccaagga ggtttgccgc cggaccgagg tccgcggtgg
     8821 tgagccggtc gatctggcgc ccgcaaagcc gcgtcatttc gtgcagcagg ggatccaccc
     8881 gcgaccgggg ggccgcgacc agctccacct gcagcttgcg gctggcggta tcgcgctcgc
     8941 gcacgacaac gtcgaaatac aggtccgctt cgctgaaggg cgtcaggcgg ttcagctggt
     9001 aggcgactcc ctggcgcagg ctggactcga gctggatcgg aagttccagg cggcgacgca
     9061 acagggcgga cggctcgagc aacaggtcga tgaccagccc ggcgcccccg cgacgttcga
     9121 tgtcatcgag ctgcgccgcc agcaggccgg cggcatgctc gtcgagcgtg cgccgctcga
     9181 ggacggcaag ctcgccgctg cgctcctgtg tatcctcgag cagcacgcat tgctcgccgc
     9241 gcatcgctat gaccacgcgc gcgggctgga gcaggaacag ctgcttcacg cgatcgggca
     9301 cgctgcgcga cagttcgccc caccatgcgc gcagtatctt gagcacatcg acgccgaaca
     9361 acgtccactt gtcagccgag ctgaaatcca cgatcacact tccccgggct tagccgtcga
     9421 catcgagtga cggaaactca ttccactgga ttatacggta tggcgcaggc acaggcccgg
     9481 tgaccaccgc ctccagtccg gccacgggcg gcgcgccccc gccctcgaag gccgccacga
     9541 cctcgatcgc ctggcgccgc ttcagcccca tggcgcccga gccctgcacc agcaggcggt
     9601 acgcgccgga acgcgaggcg gagaagaagc tgctgtccag cgccatcgcc tccgccaccg
     9661 ccgggtccgc ctgcatcgcc tgcaggtcct gctcgccgaa gcggccttcc gtatccagca
     9721 tcatcacgag ctcggggtcg gcgaagcgcg gatcgatgcc ggcgctcatg gatgccaccg
     9781 acacataagg cctcagccag tcgaccaggt cctggtcgaa accgagcacc agcccgagtt
     9841 cgtccacgct gagcagcggg gcgttcgtcg ggccgtaggc gagcccggcg ctggcatatt
     9901 catcggcctc ggcgccgttt tccgtgacga tctcgtcgcc gtcgcgccag tcgacgagcg
     9961 cgttgaccag ggcatccacg tcggacacgc catcgggata attcccgatc agcgcccgca
    10021 gcatttcggg cggcgcggca ttgaggctga cgtaggcctc ctcgggcacg aagcggtagg
    10081 cgacacgggc gttgccgagc gccagcgaca gcggcacgcc ctcgagggtg gccagcgtgt
    10141 ccggcgaggc ctggccgacc agcgcgaggt aattcagcgc ggccgtggcg cccgcgcgca
    10201 gccggatgcg tccgaccatg tcgccgacga tgcgattctc cacgcggatc tcgttgctga
    10261 acgcgccgag caagaaggtc agcagcgtca ggatccacag caccagcacg agtaccgccc
    10321 cgcgctgggc gccgcgcccg ttgcgcaagg tccgcagcgc gctcatcccg cccccacgcg
    10381 catcaccacc ggcggcatcg catgccggcc gcccgtgaag ctgatgcgca ccagttgcgg
    10441 aaactccggg cgcccggccc agctgtcgcc ccagccgatt tcctcgcccg aggcgtcggt
    10501 ggcgccgtag tactggaagc tgatcaccat gtcctcgctg atcagcgagg cttcgttatc
    10561 ggtggacgcc tcgtccatgc cctgcggcga gtagggaacg aagcgccccc agacgccggg
    10621 actgccgccc tcgtcggaaa agagttccac gacgtagagc tcgccgcccg ccgacatcga
    10681 cagcggcgcc acgtagcgca gcgacgaggc cgtgccgctg aacgcgaact cggtgcgatc
    10741 gggaaacatc agcaccaccg gcgaaacctg gaacaggtga cgatgcacga cgttgagcaa
    10801 ctgccgggcg ccctcgacgc gctgcaggtg atcctcggcg gtgcgccacg actttatgcc
    10861 ggtgcggaat gccgagaaca gcgtgagcgt gatcaggctg gtgagcacca gcacgatcag
    10921 cacctcgacc agcgtgaagc cgcgcgctcg cgccgggcag gcgttcacgg tcccacccgc
    10981 agcgactgca gcctgaagct ccccggctgg cgcccggaat cccagttcac gtccacctcg
    11041 acgaggtaga gcgccggggt ctgctcgggc atcacggcca ggccgtcggg atcctggaac
    11101 ggcgagacgc tgatgcgcca gtggtacttg tcgccctctt ccactccttc catctcgccg
    11161 acggcgaccg ggtaaaggat gccgacctcg gccaggcgcg attcggctac catcgccgcc
    11221 atgacgaatt cgtccatctt cccgagattg cgcaggccgc cggcgaaggc ctggatcagc
    11281 gccacgatgc cgatcgccgc gagcacgaag gcgaccagca cctcgagcag cgtgaatccg
    11341 gtttgccgct gcgccaatcg acgcgggatg cggggcatgc gctcgcctac tcgacgccca
    11401 ggcgcgcgac atcgaccgcg ccggtgagcc agtcgatgct gacttcgaac accacgccct
    11461 cggcaccgag ctcgatggag ccgccgctgg aggagccgtc gggatagaag cggatgcccg
    11521 ccacgtcgcc gcgcacctca cgctgcgccg tgttgaccag taccttcgcc tcctcgggca
    11581 ggcggtagcg ccgggtgcgc gtatcgacca cgtaggactt gtcggcggtg tccacccaca
    11641 gcgcgacgct gcgccgctcg ctcacggcct cggagcgcgc ccagcgcagt cccgtggcga
    11701 gatcgcgcac cgaggactgc agttgcgagc ccacgccgcg cgagagcagc ggtcccacgg
    11761 tcgtcgcggc cagggcgagg atcatcatca ccaccagcag ttcgatcagg gtgaagccgc
    11821 gcatcgcgcg agcccgcacg ccgggctcac tcccagctga cgatgtccgc gtcatccttc
    11881 tcgccgcccg ggttgttgtc ggcgccgtag gagaacacgt cgaactcccc gtgctcgccg
    11941 ggatggcggt actggtagtc gcgcccccag ggatccttgg gcacgcgcgc cttgctgaga
    12001 taggggccgt tccagccgac cacgccctgc ggctgctcga ccagcgcctg cagccccgcc
    12061 tcctgcgtgg ggtagttgcc gacctcgagc tggtacatgt gcagcgaggt gacgaggtcc
    12121 tcgatctgca ccttcgcggt atcgctcttg gcgccgccga cgtatttcat gatgcgcggg
    12181 ccgacgatgc cggccagcat cccgaggatg accagcgcca ccagcagctc catcagcgtg
    12241 aagcccgcgg cgcgggcgaa tcgggaggaa tgtctgtgca tatgcgaaaa actccttcag
    12301 tagaccagtt cgttgaccga catgatgccg accatgatcg agacgatgat gccggcgatc
    12361 agcacgccga ggcccagtat cagcgccggc tccagcagcg ccagcaggcg cttcacggcg
    12421 ccgctcacct cctcgtcgta gatggccgcc accttcagca ggatgggttc gagctgtccc
    12481 gtttcctcgc cgacctggat catgtgcatc gccagcggcg gaaagtggct gtgctcgatc
    12541 agcgcgctgc tgagcagctt gccttccttc agcgcggcca ctccctgctc gagatccgcg
    12601 cgcagcgtgc ggttgcccac ggttgccagc gcgatcgaca acgcattgac cagcaccacg
    12661 ccgccctgga gcagggtgcc cagcgtgtgt gccaggcgcg cggtctcgac cttgcgcacc
    12721 aggtcgccgg tgagcggtat gctcagcagc ttgcggtcga gacgctcgcg gaactcgggc
    12781 ttggccagca ggttcatcac cagcgcgacc gcgccgccga tcgccagcac cagcaaccac
    12841 cagtagctgg caacccagcc gcccgcggcc atcacgatgc gcgtcggcag cggcagcgtg
    12901 ccgcccatct cggtgaacat gtcggcgaac ttcggcacca cgaaaccgag caggatgatc
    12961 agcgagccgc tcgccaccag cagcaggatg gccggataca tcaacgccga gctcaccgtg
    13021 ctcttcagcg ccttggtgcg ctccatgtgc tcggcgaggc gcgtcagcgt cgactcgagc
    13081 gcgccgctgg attcgccggc acggatcatg ttgacgaaga aatcggagaa ttccgggtgc
    13141 tcgcgaaagg catcggccag cgtgccgccc gtgcgcacgc gcttgctgat cgcgccgatg
    13201 atctccttca gccgcgcgtc ggtggccagg ccctgcagca cggcgaggct gcgatcgagc
    13261 ggcaccgagg acgacagcat gatcgccagc tcgcgcgtga actccccgat cgcgttctgc
    13321 ccgccgcgcc ggcgcgagaa caggcgcgcc agcgcgtcgg tgccgccgcg cgagccgccc
    13381 gccgcctgcg gttctatcga taccggcgtc agtccctggc gcgccagctg ggcggcggca
    13441 cgctccacgc tgtcgtgctc gatctccccg cgcgtgacct tgccctcggc ggagatcgcg
    13501 cggtacctga agtgtgccat ggcgcgcctc ggcccccgct actggtgcgt cacgcgcagc
    13561 acctcgtcga tcgaggtgtg gccggcgaag gccttgagca cgccgtcttc gtacatggtc
    13621 accatgccgt tggcgcgcgc ggccttcatg atgacctcgg cgctggcgtg ctccagtatc
    13681 aggtggcgga tgctgtcatc gaccagcagc agctcgtgca cgcccatgcg cccgcggtag
    13741 ccggtgcgcg cgcaggcctc gcaacccgtc gcgcgccaga cccggcgcga tccggcgcgc
    13801 tccacgaggc gcgagatacc catgtgctgg acctcctcgt cggtgagcgc ggactcgcgc
    13861 cggcacgccg ggcacagcac gcgcaccagg cgctgcgcca tgatggcgtt gaccgtggag
    13921 gtgagcaggt aatcctcgag gcccatgtcg agcaggcgcg tgacgccgct ggccgaatcg
    13981 ttggtgtgca gcgtcgagaa cacgatgtgg cccgtcagcg ccgactggat cgcgatgccc
    14041 gcggtttcgc ggtcgcgcat ctcgccgatc atgatcacgt cgggatcctg gcgcacgatc
    14101 gagcgcagcg cgtgcgcgaa atcgagcccg atgcgcggcg ccgcctggat ctggttcacg
    14161 cccgcgatct ggtactcgac cgggtcctcc acggtcatga tcttgcgctc gggcgtgttc
    14221 atgcgcgaga gcacggcgta gagcgtcgtg gtcttgccgc tgccggtggg tccggtcacc
    14281 aggaagatgc cgttgggccg ctccagcacc gacttcaggt ccgacagcac cttgtccgag
    14341 agcccgatgt cctcgaggtt caggttcagt gtctgcttgt cgaggatacg catcaccacg
    14401 ctctcgccgt gcagcgtggg cacggtggag acgcgcaggt cgacctcctt gccgctgatc
    14461 ttcagcttga tccgcccgtc ctgcggcagg cggcgctcgg cgatgttcag gcgcgagagg
    14521 atcttcacgc gcgagatgat cgccggcgcc atgcgcgcgg gcggcgactc cacgtcctgc
    14581 agcacgccgt cgatgcggta gcggatcttc agctcgtgct cgtagggctc gatgtggata
    14641 tcggaggcgc gcgcctcgag ggcgcgactg aagaccaggt tcacgacgcg gatgaccggc
    14701 gcgtcgctgg ccatgtcctt gagccgctcg acgtcctcga cgtcttcctg cagcaccgca
    14761 tcggactgca cgccgtcgac gatctggtcc atcgccgagc gcccgccgcc gtagagccgc
    14821 tcatgcgcct gctcgaggtc cgaggccagc gccagcagcg gctcgatgcg ccgctcggtc
    14881 gcgatctcca gcgcgcgcag cacgtagtca tcgctcgggt cggccatggc cacgcgcagg
    14941 cactcgccgt cgtcggcgag cggcagcacg ttgaactgct gcaggaactc gccggatatg
    15001 cgctcctcgg cgacgggtgc ctcggggaag tcgtcgggtc cgatcagcgc gcagccggtg
    15061 agttccgaga ccgcatcggc gacctccttc tcggccacga gacccaggcg caccagcgcc
    15121 tgccccagcg tgccgccgga gatcgccagc acgcgctcgg cgcgctgcag atcgggctcc
    15181 gcgagcttgc cgcgcgcgac cagcagtttg cccagccccg aagccggccc ggtcccgttc
    15241 acgttcgccg ccttatccat cgcgttgctg caaccagtgt ttcagacgaa ttctccattg
    15301 cggcgggagc agggaaccga gcgccgacca cacgcgggtc cggcgcgggg catcataacg
    15361 gccatcgccc gcgccgtcag cttcgcctgc cagagcatca ggccccccgg ggcgcgcgaa
    15421 gttccgccgc gccggcgtaa caagttcgcc ttccccctcg tcacggcgct ggcgcgcgac
    15481 atccacggcc cggcgatacg gggccggcgg atcggcggca acgaaacgct gtatcagcgc
    15541 ctcgtagtcc gggtacagcg cggtgagcgt cttcatcgcg gcctgcttct gctctgattg
    15601 cgtgtcggcg aagctgacac caccggcgtg gtagacgaag gtatccgcgc acagcaggtg
    15661 gcgccagccc tggcgcgcgg cgcgcaggca gaactcgttt tcctcgccgt agcccctgcc
    15721 gaacagcgcc acgtcgaaga gccccacctg cgccaggcag tcgcggcgga tgtacatgca
    15781 gaaacccacc gcggtgggaa cgggcactgc ctcgccgcgg ttgacctcgc gaaacagcgc
    15841 atccagttct gccaccgaca tgccctgcgg cagggcgttg tcctcgcaga agaccgggta
    15901 gctgcagatc gtggcgttgt tggagaacgg cgtcacgctg gcggtcgcgg gctcgcgcca
    15961 ggcgcacgcg cgcagccggt cgagccagtc gttggcgacc accgtgtcgc tgttcagcag
    16021 caccacgtcg cgcgccggat gcagcgccat gccgcggttc gccgtggcca cgaaaccgag
    16081 gttcgccgcg ttctcgagca ccgtcacgcc ctccagtccg cgcaggtagc gcctcagctc
    16141 cggctcgggg ctcgcatcgt cgatcacgat gatctgcgcc gcggtgcgac agcgcgaggc
    16201 cagcacgctc tcgatgcagg cacgcgtttc cccgaggccg cgatagaccg ggatgatcac
    16261 gtcgacgcag gtctcgcgcc cgctggcgct gccggccgtg cgctcaggcg cgctcatcgg
    16321 cgggttccgg ttcgcccaca cgacaggcca ggtcctcgat ggcgatccag cccatcatcg
    16381 cgggatccgc ctccgcggtg aaatgcagcg catcgtgcac ccagtggtgc tgcacatgcc
    16441 gttcctgggt cccggcggcg atggcgacgc cgacgatata gtcgccggtg gcaaacagcg
    16501 gcatccggaa cgcgaactcc accacgaaac ggctgccggc ccgcacgaag cgccagtccg
    16561 gcagctgctt cagggtattg ccgccgagca gatattcgcc gagccggttc ttgacgaaga
    16621 atccggctat cggcgcatcg atgtcggtga acgcgcggcc gctcaggcgc aggcgcacgc
    16681 gttgtgccgc catcagcagc ttcagcggcg taccgtcctg ccccagcagc tcgacctgct
    16741 ccacccgtgc ctggccggta ccgaactgcc cggcgggatg ctccacggcc ccggcggatt
    16801 cctgcgcgtc gttgcgggcc tgctgctccg ggtacaacga gcccaggtac gcttcgcaga
    16861 cctccttggc ggggccgagg cgcgcgatgc acccgcgatc cagccagagg gccttgtcgc
    16921 acagcgtgat caccgaatgg acgtcgtgac tgacgaacac cagcgtgccg tgctccttga
    16981 actgcctgat gaagcggatg catttctgca cgaagtaggc atcgcccacc gcgagcgcct
    17041 cgtcgatgat caggatgtcg gcgttcacgt gcgcgatcac cgcgaacgcg agccgcacga
    17101 acatgccgct ggaataggtc ttcacgggct ggtcgatgaa gtcgccgatc tcggcgaacg
    17161 cgagtatcga gggcatggca tcgtcgatct cggcgcgacc gaggccgtag atggcgccgt
    17221 tcatgtagac gttctcgcgc ccggtgaact ccgggttgaa ccctgcgccc agttccagca
    17281 aggcggcgat ccggccgttc accgtcacgg tgcccgcgct cgggctcagc gtgccgcaga
    17341 tgatctgcag cagcgtcgac ttgcccgcgc cgttgcgccc gatcagggcc agcgtctcgc
    17401 cgcggcgcac gtcgaggctc atcggctgca acgcgtcaaa gcgctcgcag cgctgcgcgc
    17461 gccagccgaa gagctggtac agcaggcgat cgaaggggtg cgcgaagatg cggtagcgct
    17521 tggcgatgcc atcgaggcgg atcgcgacat cagaggacat cggcgaaccc cggctgcgcc
    17581 ttgcggaaca cctgccagcc cagcagcgcg accagcagcg cgacggccgt gaaccgcgcc
    17641 agcgtctgcc agtcgggcat cgcgccgccg agcaccaggg cgcgcagctc gccgatgacg
    17701 taggtcaggg ggttcagata caggtgctcg cgcagcccgg ccggcagcgc ttccagcggg
    17761 tacagcaccg gcgccaggaa cagcagcatc gtgctcacca ccggcagcac ctgcgcgaga
    17821 tcgcgcaggt acacgcccag tgccgcgatg aaccagctca aaccgagcgc gagcagcgcg
    17881 aacggcagca gcaacagggg cagcaacagc acgccggcat ggacctgctg ctgcacgacc
    17941 ccggccgcga cgaggagcac cagcaagccg accagcaggt ggaacagggc cgcgaacacc
    18001 agcaccaccg ggatgatcca cagcggaaac accaccttct tgacgtagcc ggtgttctcc
    18061 gcgatcagcg cgggcgaacg gttcaggcat tcggcgagga aggagtgcag aagaacgccc
    18121 gcgaacaggt tgagcgcaaa gctggcggtg tcgccatcgc cggtctgcca gcgcacgcga
    18181 aagacggtgc cgaacacgaa cgtatagacc gccagcatca ccagcggcga ggcgaaggac
    18241 cagagcaggc ccatcatcga gccccggtac cgcccgagga cctcgcggcg gctcatctgg
    18301 tagatcagga aaacgtgctg cttcagcatc gcgggcctat ccgggacgcc ggcacgcgca
    18361 gcgggacacg cgctatcgcg gcgccgcctg cacgagggcc gataccgcgg cctggttcac
    18421 ctcgatgccc tcgagcgagc gggtctcctc gaccttctgc ttgcgcaggg cggcctggaa
    18481 tttttcacgt tcgcgcgaga tgagctcggc cttcacctcg tcgtactcca gcttgtgctc
    18541 gggcttgcgc tcgccgagct tgatgacatg gaagccgaac tgcgtttcga ccacctcgct
    18601 gatctgcccc ggctcggtca gcgcgaaggc cgcctcgtcg aagggcttca ccatcttgcc
    18661 gcgctcgaag taaccgaggt cgcccccatt ggtcttgacg ctgggatcgt ccgacagctc
    18721 cagcgccagt tcctcgaacg gagtcttgcc ttccacggca cgccggcgcg cgtcctgcgc
    18781 ccgcttcagg gcctcgtcac gcccgcgctc ggcgcctacc gagaccagga tatgcgaggc
    18841 gcgcacgcgt gcgggctgcg tgaactgctc ggggttggcc acgtacttct cgcgggcgag
    18901 cttctcgaag tcgggctcgc gctccccggc gactgccgca tcgagcgcgg ccttcatcag
    18961 gcgctggtcg ctttgcatgg caatctggaa gcg
//
LOCUS       DAORUQ010000039        18976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2526_length_18976_cov_6.833413, whole genome shotgun sequence.
ACCESSION   DAORUQ010000039 DAORUQ010000000
VERSION     DAORUQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18976
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_18976_cov_6.833413"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLW13_05425"
     CDS             complement(<1..245)
                     /locus_tag="PLW13_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69845.1"
                     /translation="MDSVTKKSRHLPGTARMSWRSALAAVLAVLLPALSAFAAPVSMT
                     DIGFNALPGGKFEIRMKFDGTPPSPQSYTIDKPARIAL"
     gene            complement(260..778)
                     /locus_tag="PLW13_05430"
     CDS             complement(260..778)
                     /locus_tag="PLW13_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HQY69846.1"
                     /translation="MGAKRTASLVFCAALLGGCGGDFADLDAYMAEVKARPVGAIEPI
                     PTFKPYQSFTYSAQSMRAPFDIPLSAREAAVQVGKTVKPDLLRAREFLERFNIEGLQM
                     VGTLTQKGTLWALVKDEESGVHRVRIGNYLGKNHGRIVATTETEIAITEIVPNGVGGW
                     IERPRTIKLTEQ"
     gene            complement(778..1386)
                     /gene="pilO"
                     /locus_tag="PLW13_05435"
     CDS             complement(778..1386)
                     /gene="pilO"
                     /locus_tag="PLW13_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQY69847.1"
                     /translation="MALADSLKSLREVNLSDLTLDNIGSWPIAVKVVVWVLVFGLVCF
                     LGWNYHLKGMREQLLAVRTAETQLREEYRLKAFQVANLEALKAQLAEMEEQFGALLSQ
                     LPKDTEVPGLLEDITEKGTDAGLAFDSIALEGERAAEFYVELPININVKGGYHDLGGF
                     ASGVAGLPRIVTLHDFAITPGGNSGELSMRIQAKTYRYKGEQ"
     gene            complement(1390..1971)
                     /locus_tag="PLW13_05440"
     CDS             complement(1390..1971)
                     /locus_tag="PLW13_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQY69848.1"
                     /translation="MAKINLLPWREQRREQQRKEFLAVLGGVAAAGVLVALIGHLLIN
                     GQIDYQNERNQYLKTHIAALDKQVAEIKELQARRNQLIDRMKVIQDLQGTRPLIVRIF
                     DEIVRTLPDGVYFRGMERTGQQITLRGTAESNNRVSSLMRRLDASEWFSEPTLKGVKA
                     NPGFGEQANDFDLTVKVSMPGVTGEDGQGEAGE"
     gene            complement(1971..3032)
                     /locus_tag="PLW13_05445"
     CDS             complement(1971..3032)
                     /locus_tag="PLW13_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQY69849.1"
                     /translation="MIGLFKRKTPPVLGIDISSTSVKLLELSRNGDRYTVESYAVQPM
                     PPNSVVEKNIAEVDAVAQCLKDVVVKAKTKTSDVAVAVAGSAVITKIIEMAEGLSDDA
                     MEAQISVEADQYIPYPLDEVAIDFEIQGASERGPGQVEVLLAACRRENVDTRVEVIEA
                     ADLVPRVVDVEIYAMERAFGLIEGQLGLDEDYTVAIVDIGSTMTTLSVLSGGHTIYTR
                     EQLFGGKQLTEEIQRRYGLSVQEAGLAKKEGGLPADYEQEVLEPFREAVIQQVTRSLQ
                     FFFSSTQYNDVDHVVLAGGVAALTGLAQAVEDKLGTPTTVANPFKGMGVSSRVNSAAL
                     ANDAPALMIACGLAMRSFD"
     gene            3200..5614
                     /locus_tag="PLW13_05450"
     CDS             3200..5614
                     /locus_tag="PLW13_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQY69850.1"
                     /translation="MRSIVVWSWRLCRVVLPPIAAGVIAVTGSLLYLGPRLPSVDVLR
                     DVRFQEPLRVYTRDGLLMGEFGEMRCSPVRFEQIPQVLVQAVLAAEDDRFLSHHGVDF
                     GGLTRAAWELATTRSIQSGGSTITMQVARNYFLSRDQTFVRKFNEILLALQIERELDK
                     RQILELYLNKIFLGYRAYGVEAAAQVYYGRPIAQLSLDEAALIAGLPKAPSALNPLDN
                     PAGARARRDWILGRMLELGYIDRAAHDAALAAPVAARQHGSRIDLHAEHAAEMARAEM
                     ISRYGHEAYVDGYIVHTTIDGRLQRQAQHAVERGLLAYDRRHGFRGAEARLEGTTPGQ
                     WLPQLARIPTVAGLEPAVVTHVGEHEIDVLTARESVRIGWERGLSAARRYMSPDYRGP
                     SPTSAAELVAPGDVVRITADEQGWRLSQLPDAQAALVSLDASDGAIRALVGGLDFDYS
                     KFNRVAQARRQPGSNIKPFIYAAAMENGFTPASVINDAPIVFHDPGLEKVWRPENDSG
                     KFYGPTSLRTALVNSRNLVSIRLLQQLGVARAVDYLSNLGFERADLVPNLSLALGTPS
                     LTPLSLVSAYAILANGGYRVQPYLVGEVRRAGGELVARADPPRACPDCIGTGPAREAA
                     SIEEVLAEPAPQPEAAPQKTAPRVMDPRVAYMIDNILRDAVKHGTGRRALELKRADIA
                     GKTGTTNGPTDAWFSGYAGMVVTTAWLGFDANTPLGRQEFGGSATLPIWVDFMREATR
                     DMPTYVRPMPVGLVNVRVDRQTGLLAAPGQRDAVFELFPLENVPSVTPTGDAYPSDEY
                     NTQDIF"
     gene            complement(5616..6446)
                     /locus_tag="PLW13_05455"
     CDS             complement(5616..6446)
                     /locus_tag="PLW13_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQY69851.1"
                     /translation="MLPSAAAFLQRLSGTPVARAAGVLLCVCCLARVAAADPSCAPGP
                     GLTPARLAKVVDGDTLALADGSRLRLIGVNAPETRGSDGRPEAGAVEATRFARSFLGS
                     GRLGVVVGSDRRDRYGRTLAHVYRADGESLEAALLAAGLARQVTVPPNLGQLDCLRRA
                     ERSARAARAGLWRSGAFAPRATATLAPGESGFRLLQGRVTAVEPAGSTWWVEIDDRVS
                     LRIADADRKYFSLDELRALEGTAVEVRGWLTWRNPARARGHPPWMMQLRHPAALAPGA
                     "
     gene            complement(6466..6687)
                     /gene="rpmE"
                     /locus_tag="PLW13_05460"
     CDS             complement(6466..6687)
                     /gene="rpmE"
                     /locus_tag="PLW13_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQY69852.1"
                     /translation="MKADIHPAYDKVSANCSCGNVIETRSTLVKNLHIDVCSKCHPYY
                     TGKQKVMDTGGRIDRFKKRFGAVGTRRQD"
     gene            6876..9086
                     /locus_tag="PLW13_05465"
     CDS             6876..9086
                     /locus_tag="PLW13_05465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQY69853.1"
                     /translation="MPAEPILRVAIPAPLRHALDYLPPAGVEAAAIGAGMRVVVPLGP
                     RRLVGVITEVIARSELAPGKLRRALALPDAAPLVPPDLLALCTFAARYYHAPLGEVVV
                     NTLPVELRTVERAPRRVARCWQATTESFGLGEAALARAPKQAAALAFLQRQGPADDAA
                     CRAAGIDGATLRALEHKGLAMAAPAGHAATPAVPAPAASAAAPALNAGQQAALAALEQ
                     TCGAFHCALLEGVTGSGKTEVYLRFIERVLAAGRQALVLVPEIGLTPQTRQRFEARFG
                     HGIAVLHSGLGDRERLDTWRRCADGRARVLIGTRSALFSPLPELGAIIIDEEHDASFK
                     QQEGFRYSARDLAVVRARQLGVPVVLGSATPSTESLANCASGRFLHLRLHERAGAARP
                     PAVRLLDVRGQALEGGMCGDTIARIGAELAAGNQALVFLNRRGWAPVLSCTDCGWMGE
                     CQHCDARMTLHRAQRLLWCHHCDARAAVPLSCPHCRSARLIALGTGTERTEHALQRLF
                     PAFPIRRIDRGTMQARDAMEQLIGQLERGEPCILVGTQMLAKGHHFPHVTLVAIIDLD
                     GGLFSADFRAAERTGQLLVQVAGRAGRAERPGEVLVQTFHPGHPWLTRLVDGGYEAFI
                     EPILVERRQQGLPPYAHLAILRAESREQQPALALLQELKQELLHEHRTVQIIGPVPAP
                     MQRRAGFHRLQLLLSHAQRTPLHAALDAACGLLETRRAPGVERWHIDVDPQEGA"
     gene            9199..9936
                     /locus_tag="PLW13_05470"
     CDS             9199..9936
                     /locus_tag="PLW13_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQY69854.1"
                     /translation="MAHDFAKQRAARANRSGQPAHAPWLWLVSGIVIGTLVSFLVYLA
                     TLTPPPQQAATVSADAAGEEPAKAAATEPAKTKKAATAPETRPQFDFYDILRQTENGN
                     AAAEAATQAAQASARPAQPVEVPEPQTPAPTVAAAKPAATPATGAPPAAGAAASAPAA
                     ATPATPKPETPRAAALAMQAGAFSQHADADRRRGEILLLGYDARVEPVKGADGQTRYR
                     VTVGPFADPAALANARRALRDVGIETL"
     gene            10033..10578
                     /gene="hslV"
                     /locus_tag="PLW13_05475"
     CDS             10033..10578
                     /gene="hslV"
                     /locus_tag="PLW13_05475"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQY69855.1"
                     /translation="MEQYRGTTILSVRRHGKVVIGGDGQVSMGNTVMKSNARKVRRLH
                     EGKVIAGFAGGTADAFTLFELFEAQLDKHQGQLVRAAVELAKAWRTDRALRRLEALLA
                     VADRGNSLIITGNGDVIEPEDSLIAIGSGGPYAQSAARALLRHTDMPAREIVESSLGI
                     AADICIYTNHNRTIEELDAAP"
     gene            10624..11961
                     /gene="hslU"
                     /locus_tag="PLW13_05480"
     CDS             10624..11961
                     /gene="hslU"
                     /locus_tag="PLW13_05480"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQY69856.1"
                     /translation="MSSMTPREIVHELDRYIIGQQEAKRAVAVALRNRWRRTQLDPEL
                     AREVTPKNILMIGPTGVGKTEIARRLARLANAPFLKIEATKFTEVGYVGRDVESIVRD
                     LVDVSLKMHRESEIARVRERALDAAEERILDALLPPARPAPGAAPERDSAARQVFRKK
                     LREGELDEREIDIELTAAPVGVEIMAPPGMEEMTSQLQGMFANLSAGRSKTRRLSVRA
                     ALKMVQDEEAAKLVNEDDLKRRALEAVEQNGIVFLDEIDKVARRSEYQGADVSREGVQ
                     RDLLPLIEGCTVSTKHGTVRTDHILFIASGAFHLARPSDLIPELQGRLPIRVELGALS
                     PEEFARILTEPNASLTEQYRALLATENLAVEFTPEGIRRLAEVAWEVNERTENIGARR
                     LHTVMERLLEEASFSAADRGSSRETSRLLIDAAYVDAQLGELARNEDLSRFIL"
     gene            12002..12394
                     /locus_tag="PLW13_05485"
     CDS             12002..12394
                     /locus_tag="PLW13_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HQY69857.1"
                     /translation="MSVQVKVPETIRLHRASRVLELGYADGEHYSLGCEYLRVYSPSA
                     EVRGHGAGQETLQTGKRLVTIERIETVGNYALQFHFSDGHDSGIYSWEYLHELCREQH
                     KRWADYLARLALAGASREPAGPVLIARQ"
     gene            12416..13171
                     /gene="ubiE"
                     /locus_tag="PLW13_05490"
     CDS             12416..13171
                     /gene="ubiE"
                     /locus_tag="PLW13_05490"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQY69858.1"
                     /translation="MAEDRTTHFGYREVAEQDKARLVGGVFHSVAARYDLMNDLMSGG
                     IHRLWKRLTIELSGVREGQRVLDVAGGTGDLAARFSRIVGSSGRVVLSDINESMLGTG
                     RNKLLDHGVCANVDFVLADAEQLPFADHSFDCITIAFGLRNVTHKERALESMLRVLRP
                     GGRLLVLEFSKPTSELLGKLYDAYSFGVLPRLGKLVAGDEDSYRYLAESIRMHPDQET
                     LRDMMEDAGFGRCDYHNMTGGIVAVHRGFKADA"
     gene            13168..13827
                     /locus_tag="PLW13_05495"
     CDS             13168..13827
                     /locus_tag="PLW13_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQY69859.1"
                     /translation="MNAGASTLNVAALAALERAINAALGMDDATREQVNALAGKVFCI
                     DCSVPALRVYIAPGADSVRLYSHYEGAVSCTVSGAGSDFVALAGALDKASALVNGNLR
                     ISGDSAPLLALEKALGTLELDWEQRLALVVGDVAAHQLGRAARGSARWGRRAHGSLAR
                     HVEEFIHEEARLAPPRLEVEDFFADLRATAQRGERLEAGIRRLGRRIDALAARDRTRR
                     G"
     gene            13834..15462
                     /gene="ubiB"
                     /locus_tag="PLW13_05500"
     CDS             13834..15462
                     /gene="ubiB"
                     /locus_tag="PLW13_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HQY69860.1"
                     /translation="MSRVARLARILQVCRRYRLDTFLDTGHVPPSWRRLLRLAPFTRG
                     GDPAQSRAVRLRRALEDLGPIFIKFGQLLSTRRDLIAPDIADELSRLQDAVPPFPAAE
                     AQAIVERALGKPVEQLFASFEATPLASASVAQVHAATLFDGRRVVVKVIRPGIERTIR
                     EDVRLLYTLAHWLERFHPDGRRLRPVEVIRDYEQIVLDELDLQREAANTSQLRRNFLD
                     SDLLYVPEVHWDYTRRNVFVMERIHGIPVSDVAALNAAGVDLKELAERGVKIFFTQVF
                     RDGFFHADMHPGNIFVEVTDPARPRYIGIDCAIMGSLSDFDQYYLARNLLAIFQRRYR
                     EVAELHIECGWVPPQTRVHDFEAAMRATCEPVFEKPLGEISFGQLLVYLFQTARRFDM
                     EVQPSLVLLQKTLLHIEGLGRQLYPELDLWANAKPFLEQWVSDRYSPRGLLEKLQKRA
                     PGWLEQLPQRPEKILEALRPVEAPRAPAALAATGHGAARQGSDRWRLLELLVAAGGMV
                     LVNPDIAAQAPAQWHPATWVFAALALHALIAQRR"
     gene            15571..15975
                     /gene="hisI"
                     /locus_tag="PLW13_05505"
     CDS             15571..15975
                     /gene="hisI"
                     /locus_tag="PLW13_05505"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HQY69861.1"
                     /translation="MKAPADLAATVAWNEQGLAPAIVQDAASGRVLMLAWMNAESLRL
                     SIEEGRAIFWSRSRGQLWRKGESSGHVQTLHEVRLDCDRDAILLRVEQTGGIACHTGR
                     ESCFFLRLDEQGWQAADPVLREPEQIYGGAHE"
     gene            15968..16315
                     /locus_tag="PLW13_05510"
     CDS             15968..16315
                     /locus_tag="PLW13_05510"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HQY69862.1"
                     /translation="MSEVLARLDAVIAERRRSADPATSYVAKLCHGGLDRILKKVAEE
                     SGETLLAAKNVERGGDRQELVGETADLWFHCLVMLAKLDLQSSDVLAELERRFDLSGI
                     AEKAARQHASRAD"
     gene            16350..16580
                     /gene="tatA"
                     /locus_tag="PLW13_05515"
     CDS             16350..16580
                     /gene="tatA"
                     /locus_tag="PLW13_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQY69863.1"
                     /translation="MGGISIWQLLIILVIVVLLFGTKRLGSMGSDLGAAIRGFRKSMQ
                     DEPKPEDKRLPGEEPQKSEVIQGEAQRSEEKH"
     gene            16591..16953
                     /gene="tatB"
                     /locus_tag="PLW13_05520"
     CDS             16591..16953
                     /gene="tatB"
                     /locus_tag="PLW13_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HQY69864.1"
                     /translation="MFDIGFSELLLVGVIGLLVLGPERLPGAVRTASLWIGRIRRQFH
                     EIKTEVEREIGADEIRAQLRNEAIMDELRQSREALESSARDIRASVEPPSPSPSPERK
                     PDAVPAPGAGGTDAKAAP"
     gene            16950..17738
                     /gene="tatC"
                     /locus_tag="PLW13_05525"
     CDS             16950..17738
                     /gene="tatC"
                     /locus_tag="PLW13_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQY69865.1"
                     /translation="MSAEVPPPSPGQDGDREMPLIEHLTELRTRLIRAIVAVLAVFGA
                     LFYFANDIYQFVSEPLRRFLPEGSTMIATEVASPFLAPFKLTLVVAALLAMPFVLFQA
                     WAFVAPGMYRHEKRFAVPLLLSSILLFYGGVAFAYYFVCPMVFGFFTSMSPEGVAVMT
                     DINHYLDFVLTMFFAFGFAFEIPVATVLLVWAGITTPQALTEKRPYVIVGCFVVGMVL
                     TPPDVFSQTMLAVPMWMLFEIGLLASRWLLAEKARSAPSSAGET"
     gene            complement(17716..18153)
                     /gene="dtd"
                     /locus_tag="PLW13_05530"
     CDS             complement(17716..18153)
                     /gene="dtd"
                     /locus_tag="PLW13_05530"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQY69866.1"
                     /translation="MRALIQRVRWAAVEVDAREVGRIGPGMLVFLGVEREDDAARADA
                     IAARLLAYRVFADDGGRMNLDVRAAGGGVLLVSQFTLAADTTRGLRPGFSTAAAPEAA
                     NALYERVLGVLRERHAPVASGIFGASMQVSLCNDGPVTFLLQN"
     gene            complement(18157..>18976)
                     /gene="pip"
                     /locus_tag="PLW13_05535"
     CDS             complement(18157..>18976)
                     /gene="pip"
                     /locus_tag="PLW13_05535"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQY69867.1"
                     /translation="GCQPANRCFFDPQDYRIILFDQRGAGHSTPHAELGGNDTRALVG
                     DMERIREFLGIEKWVLFGGSWGSTLSLAYAEAHPQRVSAMILRGIFLCRPEDLAWFYQ
                     QGASRVFPDYWRDYAEHIPAAERGDFMRAYHRLLTGSNEIARMSAAKAWSLWEAQCAT
                     LRPNHDVIGHFSDPHVALALARIEAHYFVNDAFLAPDQLLREAHRIADIPGYIIHGRY
                     DMVCPVDNAFALHAAWPKAELQIIRDAGHSAAEPGITDALVRAARDVATLLAGD"
BASE COUNT         2950 a         6786 c         6398 g         2842 t
ORIGIN      
        1 agcgcgatgc gcgccggctt gtcgatggtg taggactgcg gcgacggcgg cgtgccgtcg
       61 aacttcatgc ggatctcgaa cttgccgccc ggcagcgcat tgaagccgat gtccgtcatc
      121 gagacgggag cggcgaacgc cgagagcgcc ggcagcagca cggcgagcac tgccgccagg
      181 gccgagcgcc acgacatgcg tgccgtaccg ggaaggtgac gcgatttctt ggttacgctg
      241 tccatactgt ctccgcgctc tactgttctg tcagcttgat cgttcggggg cgctctatcc
      301 agccacccac cccattgggg acgatctccg tgatggcgat ctccgtttcg gtggtcgcga
      361 cgatcctgcc gtggttcttg cccagataat tgcctatccg gacccggtgc acgccgctct
      421 cctcgtcctt gaccagcgcc cagagcgttc ccttctgggt cagcgttccc accatctgca
      481 ggccttcgat attgaagcgc tcgaggaact cgcgcgcgcg cagcaggtcg ggcttgaccg
      541 tcttgcccac ctgcacggcc gcttccctgg cgctcagcgg gatgtcgaag ggagcgcgca
      601 tggactgcgc ggaataggtg aacgactggt acggcttgaa cgtcgggatc ggctctatcg
      661 cgccgaccgg ccgcgccttg acctcggcca tgtacgcgtc caggtcggcg aaatcgccgc
      721 cgcagccgcc cagcagggcg gcacagaaaa cgaggcttgc tgttctcttg gcgcccatca
      781 ttgttcgccc ttgtatcggt acgttttggc ctggatccgc atgctcagct cgccggagtt
      841 gccaccggga gtgatcgcga aatcgtgcag ggtcacgatc cgcggcagcc ccgccacgcc
      901 gctggcgaat ccgccgagat cgtgatagcc gcccttgacg ttgatgttga tcggcagctc
      961 gacgtagaac tccgcggccc gctcgccctc gagggcgatg ctgtcgaagg cgaggcccgc
     1021 gtcggtgccc ttctcggtga tgtcctcgag caacccgggc acctcggtgt ccttgggcag
     1081 ctgcgagagc agcgcgccga actgctcctc catctcggcc agctgcgcct tcagcgcctc
     1141 gaggttcgcc acctggaacg ccttgagccg gtactcctcg cgcaactgcg tttccgcggt
     1201 tcgtaccgcc aggagttgct cgcgcatccc cttcaggtgg tagttccagc cgaggaagca
     1261 gaccagtccg aagaccagca cccacaccac gaccttgacc gcgatcggcc aggagccgat
     1321 gttgtccagc gtgagatcgg agagattgac ctcgcgcagc gacttcagtg aatccgccag
     1381 tgccatacct tattcccccg cctcgccctg gccgtcttcc ccggtcacgc ccggcatgct
     1441 caccttcacg gtcaggtcga aatcgttggc ctgctcgccg aaaccgggat tggccttcac
     1501 gcccttcaac gtcggttccg agaaccattc cgaggcatcg agccgtcgca tcaggctgga
     1561 aacgcggttg ttggactccg ccgtgccgcg cagcgtgatc tgctgcccgg tgcgttccat
     1621 gccgcggaag tacacgccgt cgggcagcgt gcgcacgatc tcgtcgaaga tgcgcacgat
     1681 cagcgggcgc gtgccctgca ggtcctggat caccttcatg cgatcgataa gctggttgcg
     1741 ccgtgcctgc agttccttga tctcggccac ctgcttgtcg agcgcggcga tgtgggtctt
     1801 caggtactgg ttgcgctcgt tctggtagtc gatctgcccg ttgatgagca ggtggccgat
     1861 caacgccacc agcacgccgg cggcggccac gccgccgagg acggccagga actccttgcg
     1921 ctgctgctcg cgccgctgct cgcgccacgg taacaggttg atctttgcca tcagtcgaag
     1981 ctcctcatcg cgaggccaca ggcaatcatc agcgcggggg catcgttggc cagcgccgcg
     2041 gagttcacgc gcgaggacac gcccatgccc ttgaacgggt tggcaaccgt cgtcggcgtg
     2101 ccgagcttgt cctccactgc ctgcgcgagg cccgtgagcg ccgcgacccc gcccgccagc
     2161 acgacgtgat ccacgtcgtt gtactgcgtc gaggagaaga agaactgcaa cgaacgggtg
     2221 acctgctgga tcacggcttc gcgaaacggc tccagcactt cctgctcgta atcggccggc
     2281 aggccgcctt ccttcttcgc cagccccgcc tcctgcacgc tcaggccgta gcggcgctgg
     2341 atctcctcgg tgagctgctt gccaccgaag agctgttcgc gggtgtagat cgtgtgtcca
     2401 ccgctcagca cgctgagcgt ggtcatggtc gagccgatgt cgacgatggc gacggtgtag
     2461 tcctcgtcga ggccgagctg gccctcgatg agcccgaagg cgcgctccat cgcgtagatc
     2521 tcgacgtcca ccacgcgcgg caccaggtcg gcggcctcga tgacctcgac ccgcgtatcg
     2581 acgttctcgc gccggcaggc ggcgagcagc acctcgacct ggcccggtcc gcgctcggac
     2641 gcgccctgga tttcgaaatc gatggccacc tcgtccagcg gatagggaat gtactggtcc
     2701 gcctcgacgc tgatctgcgc ttccatggcg tcatcggaga ggccctcggc catctcgatg
     2761 atcttggtga tgaccgcgga gcccgccacg gcgaccgcga cgtcactggt cttggtcttg
     2821 gccttgacca ccacgtcctt gagacactgg gcgaccgcat cgacctcggc gatgttcttt
     2881 tcgaccaccg aattcggcgg catcggctgt accgcgtagc tctccacggt gtagcgatcg
     2941 ccgttgcgac tgagctccag gagcttcacg gaggtcgagc tgatatcgat ccccagaacc
     3001 ggtggcgtct tccgtttgaa tagccctatc acgttgtttt tcctggtgaa tccgggagcc
     3061 ttgctggcct cgcgagatgg cgccggcagc gaagcaagct taagcgtagt tcaaatccat
     3121 aataagaaaa gaaaatgttt ataatcgtcc gcaagcctgt ttttccggcc attccgggcc
     3181 ttttgagtca ccaaatccga tgcgttcgat tgtagtctgg agctggcgcc tgtgccgcgt
     3241 ggtgctgccg ccgattgccg caggagtgat cgccgtcaca ggctcgctgc tgtatctggg
     3301 cccgcggctg ccgtcggtgg atgtgttgcg cgacgtcagg ttccaggagc cgctgcgcgt
     3361 ctacacccgc gacggcctgt tgatgggcga gttcggcgag atgcgctgct cgccggtgcg
     3421 cttcgagcag atcccccagg tgctcgtcca ggcggtgctc gccgccgagg acgaccgttt
     3481 cctgagccac cacggcgtcg atttcggcgg gctgacgcgc gcggcgtggg aactcgcgac
     3541 cacccgcagc atccagtcgg gcggcagcac catcacgatg caggtcgcgc gcaactactt
     3601 cctcagccgc gaccagacgt tcgtgcgcaa gttcaacgag atcctgctcg cgctgcagat
     3661 cgagcgcgag ctcgacaagc ggcagatcct cgagctctat ctcaacaaga tattcctcgg
     3721 ctaccgcgcc tacggcgtcg aggccgcggc tcaggtctac tacgggcgcc cgatcgcgca
     3781 gctgtcgctg gatgaggccg cgctgatcgc cggcctgccg aaggcgccct cggcgctgaa
     3841 tccgctcgac aatcccgccg gtgcccgcgc gcggcgcgac tggattctcg ggcgcatgct
     3901 ggagctcggc tacatcgatc gcgccgcgca cgatgccgcg ctcgccgcgc cggtcgccgc
     3961 gcgccagcac ggctcgcgca tcgatctgca tgccgagcac gccgcggaaa tggcgcgcgc
     4021 cgagatgatc tcccgctatg gccacgaggc ctatgtcgat ggctacatcg tgcacaccac
     4081 catcgacggc cgcctgcagc ggcaggccca gcatgcggtg gagcgcggac tgctcgcata
     4141 cgacaggcgc cacggcttcc gcggcgccga ggcgcgactc gagggcacga cccccggtca
     4201 gtggctgccc cagctcgcgc gcatccccac ggtggccggt ctcgagcccg cggtggtcac
     4261 gcacgtcggc gagcacgaga tcgacgtgct cacggcgcgc gagtcggtgc gcatcggctg
     4321 ggaacgcgga ctgtccgccg cgcggcgcta catgagcccc gactaccgcg gcccctcgcc
     4381 cacctccgcc gccgagctcg tggctcccgg cgacgtggtc aggatcacgg ccgacgagca
     4441 gggctggcga ctcagccaac tgcccgacgc gcaggcggcc ctggtgtcgc tggacgcctc
     4501 ggacggcgcg atccgcgcgc tggtcggagg cctcgacttc gactacagca agttcaaccg
     4561 cgtcgcgcag gcgcggcgcc agccgggctc gaacatcaag ccgttcatct atgccgcagc
     4621 gatggagaac ggcttcacgc cggcatcggt catcaacgac gccccgatcg tgttccacga
     4681 cccgggactc gagaaggtct ggcggcccga gaacgactcc ggcaagttct acggcccgac
     4741 cagcctgcgc accgcgctgg tgaactcgcg caacctggtc tcgatccggc tgctgcagca
     4801 gctcggtgtc gccagggccg tcgactacct gagcaacctc ggcttcgagc gcgccgacct
     4861 cgtgcccaac ctctcgctcg cgctcggcac accgagcctg accccgctca gcctggtgtc
     4921 cgcctacgcg atcctcgcca atggcggcta cagggtgcag ccctacctcg tgggcgaagt
     4981 gcgtcgcgcc ggcggcgagc tggtcgcgcg cgccgatccg cctcgcgcct gccccgactg
     5041 catcggcacc gggccggcgc gcgaagccgc ctcgatcgag gaagtcctgg ccgaacccgc
     5101 gccgcagccc gaggctgcgc cgcagaagac cgccccgcgc gtcatggatc cgcgcgtcgc
     5161 ctacatgatc gacaacattc tcagggacgc cgtgaagcac ggcaccgggc gccgggcgct
     5221 ggagctgaaa cgcgccgaca tcgccggcaa gaccggcacc acgaacggac cgaccgacgc
     5281 ctggttctcc gggtacgcgg gcatggtcgt cacgacggcc tggctcggtt tcgacgccaa
     5341 cacgccgctc gggcgccagg agttcggcgg ttccgccacg ctgcccatct gggtcgactt
     5401 catgcgcgaa gccacccgcg acatgccgac ctacgtgcgg ccgatgccgg tagggctggt
     5461 caacgtgcgg gtcgatcgcc agaccggcct gctggccgcg cccggccagc gcgatgcggt
     5521 gttcgaactc tttccgctgg agaacgtgcc ctcggtgaca ccgaccggag acgcctaccc
     5581 ctccgacgag tacaacacgc aggacatttt ctgaatcagg cgcccggcgc gagcgccgcc
     5641 gggtggcgca actgcatcat ccaaggcgga tgtccgcgcg cgcgcgcggg attgcgccag
     5701 gtcagccagc cgcgcacctc gacggcggtg ccctcgagtg cgcgcagttc atcgaggctg
     5761 aagtatttgc ggtcggcgtc cgcgatgcgc agcgacacgc gatcatcgat ctcgacccac
     5821 cacgtcgatc ccgccggctc gaccgcggtc acgcgcccct gcagcaggcg aaaaccgctc
     5881 tccccgggcg ccagggtcgc ggtcgcgcgc ggcgcgaagg cgccgctgcg ccacagcccg
     5941 gcacgcgcgg cgcgcgcgct gcgttcggcg cggcgcaggc agtccagctg gccgaggttg
     6001 ggcggcaccg tcacctggcg cgcgaggccg gcagcgagca gcgcggcctc gaggctctcg
     6061 ccgtcggcgc ggtagacgtg cgccagcgtg cggccgtagc ggtcgcgccg atccgagccg
     6121 accacgacgc ccaagcgccc ggagccgagg aagctgcgcg cgaagcgggt ggcttcgacg
     6181 gcccccgcct ccgggcggcc gtcgctgccg cgcgtctcgg gggcgttgac gccgatcagc
     6241 ctgaggcgac tgccgtcggc gagtgccagc gtgtcgccat cgaccacctt ggccaggcga
     6301 gcgggcgtga gtccgggacc gggagcacag gaaggatccg ccgcggcgac ccgcgccagg
     6361 cagcagacgc acagcaggac gccggccgct cgcgcgaccg gcgtcccgga cagcctctgc
     6421 aggaaggcgg cagcgctcgg caacaatgcc cggccgccgc aggaatcagt cctgacggcg
     6481 cgtgccgaca gcgccgaaac gcttcttgaa acggtcgata cgaccgccgg tgtccatcac
     6541 cttctgcttg cccgtgtagt acgggtggca tttcgagcag acgtcgatat gcaggttctt
     6601 cacgagcgtg gagcgcgtct cgatcacgtt gccgcagctg cagttggcgg aaaccttgtc
     6661 gtaagcggga tgtatatcgg ctttcatcgt gtcaccttgc cgcgccctgg cgcccgccct
     6721 gctctggccg gggggaatcg ccgcgccgtt ggaataaaaa agagcgcggc atactaagca
     6781 ctgggacccg cgatggcaag ccgcccggtc ggcggctgtg gaaaataccc gtccgggggc
     6841 tggacgggcc gcggacgcgc ccataccctc cgaccatgcc cgccgaaccg atcctgcgcg
     6901 tcgccatccc cgcaccgctg cgccacgccc tcgactacct gccgccggcc ggggtcgagg
     6961 cggccgcgat cggcgcgggc atgcgcgtcg tggtgccgct gggaccgcgg cgcctggtcg
     7021 gcgtgatcac cgaggtgatc gcgcgcagcg agctggcgcc ggggaagctg cgccgcgccc
     7081 tggcgctacc cgacgccgca ccgctcgtgc cgccggacct gctcgcgctc tgcaccttcg
     7141 ccgcccgcta ctaccacgcg ccgctcggcg aggtggtcgt gaacacgctg ccggtggaac
     7201 tgcgcacggt cgagcgcgcg ccgcggcgcg tcgcgcgctg ctggcaggcg acgacggaga
     7261 gtttcgggct gggcgaggcg gcgctcgcac gcgccccgaa gcaggccgcg gcactcgcgt
     7321 tcctgcagcg acagggcccc gcggacgatg ccgcctgccg cgccgccgga atcgacggcg
     7381 cgacattgcg cgcgctggag cacaagggcc tggcgatggc cgcaccggcg ggccacgccg
     7441 cgacgcccgc ggtgccggcg ccagcggcca gcgccgcggc gccggcactg aacgccgggc
     7501 agcaggccgc gctggccgcg ctggagcaga cctgcggcgc cttccactgc gcgctgctcg
     7561 agggcgtgac cggcagcggc aagaccgagg tgtacctgcg cttcatcgag cgcgtgctcg
     7621 ccgcggggcg ccaggcgctg gtgctggtac ccgagatcgg cctcaccccg cagacgcgcc
     7681 aacgcttcga ggcacgcttc gggcacggca tcgcggtgct gcactcgggg cttggcgacc
     7741 gcgaacgcct cgacacctgg cgccgctgcg ccgacggccg ggcgcgggtg ctgatcggca
     7801 cgcgctcggc actgttcagc ccgctgcccg aactcggggc gatcatcatc gacgaggagc
     7861 acgatgcctc gttcaagcag caggaaggct tccgctactc ggcgcgcgac ctcgcggtgg
     7921 tgcgcgcgcg ccagctcggc gtgcccgtgg tactcggttc cgccacgccc tccaccgaga
     7981 gcctcgcgaa ctgcgcgagc ggccgcttcc tgcacctgcg gctgcacgag cgcgccggtg
     8041 ccgcgcgccc gcccgcggtg cgactgctcg acgtcagggg gcaggcgctc gagggcggca
     8101 tgtgcggcga taccatcgcg cgcatcggcg cagagctcgc cgcgggcaac caggcgctgg
     8161 tgttcctgaa ccgccgcggc tgggcaccgg tgctgagctg caccgactgc ggctggatgg
     8221 gcgagtgcca gcactgcgac gcgcgcatga cgctgcaccg cgcccaacgg ctgctgtggt
     8281 gccatcactg cgatgcgcgc gcagcggtac cgctgagctg cccgcactgt cgcagcgcgc
     8341 gcctgatcgc gctcggcacc ggcaccgagc gcaccgagca cgcactgcag cgcctgttcc
     8401 ccgccttccc catccggcgc atcgatcgcg gcacgatgca ggcacgcgac gcgatggaac
     8461 agctcatcgg ccagctcgag cgcggcgagc cctgcatcct ggtcggcacg cagatgctgg
     8521 ccaaggggca tcacttcccg cacgtgacgc tggtcgcgat catcgacctc gacggcgggc
     8581 tgttcagcgc cgacttccgc gccgccgagc gcaccgggca gctgctggta caggtcgccg
     8641 ggcgcgccgg gcgcgccgag cgtcccggcg aggtactggt gcagaccttc cacccggggc
     8701 atccctggct gacgcgactg gtggacgggg gctacgaggc attcatcgag ccgatcctcg
     8761 tcgaacgccg ccagcagggg ctgccgccct acgcgcatct ggcgatcctg cgcgccgaga
     8821 gccgcgagca gcagcccgcg ctcgcgctcc tgcaggagct caagcaggag ttgctgcacg
     8881 agcaccgcac ggtgcagatc atcggccccg tgccggcgcc gatgcagcgc cgcgccgggt
     8941 tccaccggct gcagctgctg ctgtcgcacg cgcagcgcac cccgctgcac gccgccctcg
     9001 atgccgcgtg cgggctgctc gagacccggc gcgcgcccgg cgtcgaacgc tggcacatcg
     9061 acgtggaccc gcaggaaggc gcctgaaaac gcggcgccgt ttggcaacag cccacgcgat
     9121 actgataatg cgcactcccg ggccgccggc ccgcccgtag ccgcagcgct ccggcgcacc
     9181 cacactgcag attcgaccat ggcccatgac ttcgccaaac aacgtgccgc gcgggcgaac
     9241 cgctccggcc agcccgcgca cgcaccgtgg ctctggctcg tcagcggcat cgtgatcggc
     9301 acgctggtca gcttcctggt ctacctcgcc acgctcaccc cgccgccgca gcaggcggca
     9361 acggtgagcg ccgacgcggc gggggaggaa ccggcgaagg cagcggctac cgagcccgcg
     9421 aagacgaaaa aagctgcgac agcccccgaa accaggccgc agttcgattt ctacgacatc
     9481 ctgcgccaga cggagaacgg caacgctgcg gccgaggccg cgacgcaggc agcccaggcc
     9541 agcgccaggc cagcacaacc cgtggaggtg cccgagccgc agacgcccgc accgacggtc
     9601 gccgccgcga agcccgcggc cacgccggcg accggagcgc ctcccgctgc aggcgccgca
     9661 gcaagcgctc ccgccgccgc gacgcctgca acgccgaagc cggaaacacc cagggccgcc
     9721 gcgctcgcga tgcaggccgg cgccttcagc cagcatgccg acgcggaccg ccgccgcggc
     9781 gagatcctgc tgctcggcta cgacgcccgc gtcgagcccg tcaagggcgc cgacggccag
     9841 acccgctacc gcgtcacggt cggccccttc gccgaccccg cggcgctcgc caacgcgcgc
     9901 cgcgcgctgc gcgacgtggg catcgagacg ctgtgaacgc acggcgcact tgaaacggcg
     9961 cgcccgcgcc cgcatatcgt ttcatcaggc gcgctgcgcc acatcgcacc tggatcacga
    10021 gaggagagcg ccttggagca gtatcgggga accaccatcc tctcggtcag gcgccacggc
    10081 aaggtcgtga tcggcggcga cggccaggtg tcgatgggca ataccgtcat gaagtccaac
    10141 gcacgcaagg tgcgccgcct gcacgagggc aaggtcatcg cgggcttcgc cggcggcacc
    10201 gccgacgcgt tcacgctgtt cgagctcttc gaggcgcaac tcgacaagca ccaggggcaa
    10261 ctggtgcgcg ccgcggtcga gctggccaag gcctggcgaa ccgatcgcgc cctgcgccgg
    10321 ctcgaggcgc tgctcgcggt ggctgaccgc ggcaactcgc tgatcatcac cggcaacggc
    10381 gacgtgatcg agcccgagga cagcctgatc gcgatcggct ccgggggacc ctacgcgcag
    10441 tccgccgcgc gtgccctgct gcgccacacc gacatgccgg cgcgcgagat cgtcgagagc
    10501 agcctcggca ttgccgccga catctgcatc tacaccaacc acaaccgcac catcgaagag
    10561 ctcgacgccg cgccctgagg cgcggcgcga caccaccgcc cgcacaccaa cggtattgcg
    10621 cccatgtcca gcatgacccc ccgcgagatc gtccacgaac tcgaccgcta catcatcggc
    10681 cagcaggaag ccaagcgtgc cgtcgcggtg gcgctgcgca atcgctggcg ccgcacgcaa
    10741 ctcgaccccg agctcgcgcg cgaggtcacg ccgaaaaaca tcctgatgat cggcccgacc
    10801 ggtgtcggca agaccgagat cgcgcgccgc ctcgcgcgcc tcgccaacgc gcccttcctc
    10861 aagatcgagg ccaccaagtt caccgaggtc ggctacgtgg ggcgcgacgt ggagtcgatc
    10921 gtgcgcgacc tggtcgacgt atcgctgaag atgcaccgcg agtccgagat cgccagggtg
    10981 cgcgaacgcg cgctggatgc cgccgaggaa cgcatcctcg atgcgctgct gccgccggcg
    11041 cgcccggcgc cgggcgccgc acccgagcgc gattccgccg cgcgccaggt gtttcgcaag
    11101 aaactgcgcg agggcgagct cgacgagcgc gagatcgaca tcgagctcac ggcagcgccg
    11161 gtgggcgtcg agatcatggc cccgcccggc atggaggaga tgaccagcca gctgcagggc
    11221 atgttcgcga acctgtcggc cgggcgcagc aagacgcgcc gcctcagcgt gcgcgccgca
    11281 ctgaaaatgg tccaggacga ggaggcggcg aagctcgtca acgaagacga cctcaagcgc
    11341 cgcgcgctgg aggcggtgga gcagaacggc atcgtgttcc tcgacgagat cgacaaggtc
    11401 gcgcggcgct ccgagtacca gggcgccgac gtctcgcgcg agggcgtgca gcgcgacctg
    11461 ctgccgctga tcgagggctg caccgtgagc accaagcacg gcacggtgcg caccgaccac
    11521 atcctgttca tcgcctccgg cgctttccac ctcgccaggc cctcggacct gatccccgaa
    11581 ctgcagggcc gcctgccgat ccgcgtcgag ctcggcgcgc tcagtcccga ggagttcgcg
    11641 cgcatcctca ccgagcccaa tgcctccctc accgagcagt accgcgcgct gctggcaacc
    11701 gagaacctcg cggtggaatt cacgcccgag ggcatccggc gcctcgccga ggtggcctgg
    11761 gaagtgaacg agcgcaccga gaacatcggg gcgcggcgcc tgcacacggt gatggagcgc
    11821 ctgctcgagg aggcctcctt cagcgccgcc gatcgcggct cgagccgcga gaccagccgc
    11881 ctcctcatcg acgccgccta cgtcgacgcg cagctcggag agcttgcgcg caatgaggat
    11941 ttgagccgct ttatcctgta agctagcggg ccagtccagg ggaggagcgt cgggcgcgag
    12001 catgagcgta caggtcaagg tgcccgagac gatccgcctg caccgggcat cacgcgtgct
    12061 ggagctcggc tatgccgacg gcgagcatta cagcctgggt tgcgagtacc tgcgcgtgta
    12121 ctcgccctcg gccgaggtgc gcggccacgg cgccggacag gaaacgctgc agaccggcaa
    12181 gcgcctggtc accatcgagc gcatcgagac cgtgggcaac tacgccctgc agttccactt
    12241 ttccgacggc cacgacagcg gcatctactc ctgggagtac ctgcacgagc tgtgccgcga
    12301 gcagcacaag cgctgggccg attacctcgc acgcctcgcg ctcgccggcg cctcgcgcga
    12361 accggcgggc cccgtcctga tcgccaggca atgagcacgc gccacggaga cgcccatggc
    12421 tgaagaccgc accacgcatt tcggctatcg cgaagtcgcc gagcaggaca aggcgcgcct
    12481 cgtcggcggc gtattccact cggtggcggc gcgctacgac ctcatgaacg acctgatgtc
    12541 gggcggcatc caccgcctgt ggaagcgcct gacgatcgag ctctccggcg tgcgcgaggg
    12601 acagcgcgtg ctcgacgtcg ccggcggcac cggcgatctc gcggcgcgct tctcgcgcat
    12661 cgtgggcagt tcgggccgcg tggtgctctc ggacatcaac gagtccatgc tcggtaccgg
    12721 gcgcaacaag ctgctcgacc acggggtgtg cgccaacgtc gatttcgtgc tcgccgacgc
    12781 cgagcagctg cccttcgccg atcacagctt cgactgcatc acgatcgcct tcggcctgcg
    12841 caacgtcacg cacaaggagc gcgcgctgga atccatgctg cgcgtgctgc gccccggcgg
    12901 acggctgctg gtgctggagt tctcgaaacc cacgagcgag ctgctcggca agctctacga
    12961 tgcctactcc ttcggcgtgc tgcccaggct cggcaagctc gtggccggcg acgaggacag
    13021 ttaccgctac ctagccgaat cgatccgcat gcatccggac caggaaacac tgcgcgacat
    13081 gatggaggac gcgggcttcg ggcgctgcga ctaccacaac atgaccggcg gcatcgtggc
    13141 ggtgcaccgc ggcttcaagg ccgacgcatg aatgccggcg cctcgacgct gaacgtcgcg
    13201 gcgctcgccg cgctggagcg cgcgatcaat gccgccctcg gcatggacga cgccacgcgc
    13261 gagcaggtga acgcgctcgc gggcaaggtg ttctgcatcg actgcagcgt gccggcgctg
    13321 cgcgtctaca tcgcgcccgg ggcggacagc gtgcgactct acagccatta cgagggcgcg
    13381 gtgtcctgca ccgtgagcgg cgccggatcg gatttcgtgg cgctcgccgg cgccctcgac
    13441 aaggccagcg cgctggtgaa cggcaacctg cgcatcagcg gcgacagcgc cccgctgctc
    13501 gcgctcgaga aggcgctcgg cacgctcgag ctcgactggg aacagcgcct ggcgctcgtg
    13561 gtcggcgacg tcgccgcgca ccagctcggg cgcgccgcgc gcggctcggc gcgctgggga
    13621 cgccgggcgc acggctcgct cgcgcgccac gtcgaggagt tcatccacga agaggcgcgc
    13681 ctcgcgccgc cgcggctcga ggtcgaggat ttcttcgccg acctgcgcgc caccgcgcag
    13741 cgcggcgaac ggctggaggc ggggatccgt cgcctcggcc gacgcatcga cgcactcgcc
    13801 gcccgcgacc gcacgcgtcg cggataagcc acgatgtcgc gcgtggcgcg cctcgcgcgc
    13861 atcctccagg tctgccggcg ctaccgtctc gacaccttcc tcgacaccgg gcacgtgccg
    13921 ccgtcctggc gaaggctcct gcgcctggcg ccgttcaccc ggggcgggga tcccgcgcaa
    13981 tcgcgcgccg tcaggctgcg ccgcgccctc gaggacctgg ggccgatctt catcaagttc
    14041 gggcagctgc tgtcgacgcg gcgcgacctg attgcgcccg atatcgccga cgaactgagc
    14101 cgcctgcagg atgccgtgcc gccctttccc gccgccgagg cgcaggccat cgtggagcgc
    14161 gcgctcggca agccggtgga acagctgttc gcgagcttcg aggccacacc gctcgcctcg
    14221 gcctcggtcg cgcaggtgca cgccgccacg ctgttcgatg gccggcgcgt cgtggtgaag
    14281 gtgatccgcc ccggcatcga gcgcacgatt cgcgaggacg tccgcctgct ctacacgctg
    14341 gcgcactggc tcgagcgctt ccatccggac gggcggcgcc tgcgcccggt cgaggtgata
    14401 cgcgattacg agcagatcgt gctcgacgag ctcgacctgc agcgcgaggc ggccaacacc
    14461 tcgcagctgc ggcgcaactt cctcgactcg gacctgctct acgtgcccga ggtgcactgg
    14521 gactacacgc gacgcaacgt gttcgtgatg gagcgcatcc acggcatccc ggtatccgat
    14581 gtcgcggcgc tgaatgccgc gggcgtcgac ctcaaggagc tcgccgagcg cggcgtgaag
    14641 atcttcttca cgcaggtgtt ccgcgacggc ttcttccacg ccgacatgca ccccgggaac
    14701 atcttcgtgg aggtcaccga tccggcgcgc ccgcgctaca tcggcatcga ctgcgcgatc
    14761 atgggcagcc tcagcgattt cgaccagtac tacctcgcgc gcaacctgct cgcgatcttc
    14821 cagcgccgct accgcgaggt cgccgagctg cacatcgagt gtggttgggt gccgccgcag
    14881 acccgcgtgc acgatttcga ggccgcgatg cgcgccacct gcgagccggt cttcgagaaa
    14941 ccgctcgggg agatcagctt cggccagctg ctggtctacc tgttccagac cgcgcgccgc
    15001 ttcgacatgg aggtccagcc ctcgctggta ctgctgcaga agacgctgct gcacatcgag
    15061 gggctcgggc gccagctcta ccccgagctc gacctgtggg cgaacgccaa gcccttcctc
    15121 gagcaatggg tgagcgaccg ctattcgccg cgcgggctgc tcgagaagct gcaaaagcgc
    15181 gccccgggct ggctcgagca gctgccgcag cgcccggaga agatcctcga ggcgctgcgg
    15241 cccgtcgagg cgccgcgcgc cccggcggcg ctggccgcaa cgggccacgg cgcggcgcgc
    15301 cagggcagcg atcgctggcg gttgctggaa ctgctggtcg cggcgggcgg catggtgctg
    15361 gtgaacccgg atatcgccgc ccaggcgccc gcacaatggc atccggcgac atgggtgttc
    15421 gcggcgctgg cgctgcatgc gctgatcgcg cagcgccggt gaatcccggc gggcgggtcg
    15481 gcattcatgc cgacgggtgc ttgcccggat gctgctgtcg ccatgaatgg cgacccacat
    15541 gatgccgtca acctgccata atcctcgccc atgaaagcgc ccgccgatct cgccgccacc
    15601 gtcgcctgga acgaacaggg cctcgcgccc gccatcgtgc aggacgccgc ctcgggtcgc
    15661 gtgctgatgc tggcctggat gaacgccgag tcgctgcgac tgagcatcga ggaaggccgc
    15721 gcgatcttct ggtcgcgctc gcgcgggcaa ctgtggcgca agggcgagag ttcgggccac
    15781 gtgcagacgc tgcacgaagt gcgcctggac tgcgacaggg acgcaatact gctgcgggtg
    15841 gaacagaccg ggggtatcgc ctgtcatacc ggacgcgaga gctgtttttt cctgcgcctc
    15901 gacgagcagg gctggcaggc ggcggacccg gtgctgcgcg agcccgaaca gatctacggg
    15961 ggcgctcatg agtgaggtgc tggcacgact ggatgcggtg atcgccgaac gcaggcgcag
    16021 cgccgacccc gccacttcct acgtcgcgaa gctctgccac ggcgggctgg atcgcatcct
    16081 gaagaaggtc gccgaggaga gcggcgagac cctgctcgcg gcgaagaacg tcgagcgcgg
    16141 cggtgaccgc caggaactcg tcggcgaaac cgcggatctc tggttccatt gcctggtcat
    16201 gctggccaag ctcgatttgc agagttcgga cgtgctggcc gagctcgagc ggcgtttcga
    16261 cctgtcggga atcgccgaga aggcggcccg gcaacacgca tcgcgcgcgg actgaggttg
    16321 cgcgcctcac acgggagaac ggtatgggat tgggcggcat cagtatctgg cagcttctga
    16381 tcatcctggt gatcgtcgtg ctgctctttg gcaccaagcg gctcggctcg atgggatcgg
    16441 acctcggcgc ggccatccgc ggctttcgca agtcgatgca ggacgaaccc aagcccgagg
    16501 acaagcgcct gcccggcgag gagccgcaga agtccgaagt gatccagggc gaagcgcagc
    16561 gcagcgaaga gaagcactga ggcagcaccg gtgttcgaca tcggtttttc ggagctgctg
    16621 ctcgtcggcg tgatcggcct gctcgtgctc ggccccgagc gactgcccgg ggcagtgcgc
    16681 acggcctcgt tgtggatcgg gcggatccgg cggcagttcc acgagatcaa gaccgaggtc
    16741 gagcgcgaga tcggcgccga cgagatccgc gcgcagctgc gcaacgaggc gatcatggac
    16801 gagctgcgcc agtcgcgcga ggcgctggag tcctcggcgc gcgatatccg cgccagcgtc
    16861 gaaccgccgt cgccgtcgcc gtcgccggag cgcaagcccg atgccgtgcc ggcgccgggc
    16921 gccggaggca ccgacgccaa ggccgcccca tgagcgccga agtgccgccg ccgtctcccg
    16981 ggcaggacgg cgaccgcgag atgcccctga tcgagcacct gaccgagctg cgcacgcggc
    17041 tcattcgcgc gatagtcgcc gtgctggcgg tgttcggcgc gctgttctac ttcgccaacg
    17101 acatctacca gttcgtctcg gagccgctgc gccgcttcct gccggaaggc tcgacgatga
    17161 tcgccaccga ggtcgcctcg cccttcctcg cgccgttcaa gctcacgctg gtcgtggcgg
    17221 cgctgctggc gatgcccttc gtgctgttcc aggcctgggc cttcgtggcg cccggcatgt
    17281 accgccacga gaagcgcttc gcggtgccgc tgctgctttc gagcatcctg ctgttctacg
    17341 gcggggtcgc tttcgcctac tacttcgtgt gcccgatggt gttcggattc ttcaccagca
    17401 tgagccccga gggcgtggcg gtgatgaccg acatcaacca ctacctcgac ttcgtgctga
    17461 cgatgttctt cgcgttcggc ttcgccttcg agatcccggt ggcgacggtg ctgctggtgt
    17521 gggcgggcat cacgacgccg caggcgctga ccgagaaacg cccctacgtg atcgtcggct
    17581 gcttcgtggt cggcatggtg ctcacgcctc ccgacgtgtt ctcgcagacc atgctggcgg
    17641 tgccgatgtg gatgctgttc gagatcggcc tgctcgcaag ccgctggctg ctggccgaga
    17701 aggcgcgcag cgcgcctagt tctgcaggag aaacgtgacc ggcccgtcgt tgcacaggct
    17761 cacctgcatc gaggcgccga aaatgccgct cgccaccggg gcgtgccttt cgcgcaagac
    17821 acccagcacc cgttcgtaga gcgcattcgc cgcctccggc gccgccgccg tgctgaagcc
    17881 cgggcgcagg ccccgcgtgg tatcggccgc cagcgtgaac tgcgacacga gcagcacgcc
    17941 gcctcccgcg gcgcgcacgt cgaggttcat gcgacccccg tcatccgcga acacgcggta
    18001 ggccagcagg cgcgccgcga tcgcatcggc gcgggccgcg tcgtcctcgc gctccacgcc
    18061 gaggaacacc agcatccccg gcccgatgcg cccgacctcc ctcgcatcga cctcgaccgc
    18121 cgcccagcgc acgcgctgga tcagggcacg cacggatcag tcccctgcga gcagcgtggc
    18181 gacgtcgcgc gcggcgcgca ccagcgcatc ggtgatgccg ggttcggcgg ccgagtggcc
    18241 ggcgtcgcgg atgatctgca gttccgcctt cggccatgcc gcgtgcagcg cgaatgcgtt
    18301 gtcgaccggg cagaccatgt cgtagcggcc gtgaatgatg tagccgggaa tgtccgcgat
    18361 gcgatgcgcc tcgcgcagca gctggtccgg cgcgaggaag gcatcgttga cgaagtaatg
    18421 cgcctcgatg cgcgccagcg ccagcgcgac atgcggatcg gagaaatgcc cgatcacgtc
    18481 gtggttcggc cgcagcgtgg cgcactgcgc ctcccacagt gaccaggctt tcgccgccga
    18541 catgcgcgcg atctcgttcg agccggtcag caggcgatga taggcgcgca tgaaatcgcc
    18601 gcgctccgcg gccgggatgt gctcggcgta gtcgcgccag taatcgggga aaacgcggct
    18661 cgcgccttgc tggtagaacc acgcgaggtc ctcgggccgg cacaggaaga tgccgcgcag
    18721 gatcatcgcg ctgacgcgct gcggatgcgc ctcggcatac gcgagactga gcgtgctgcc
    18781 ccaggaaccg ccgaacagca cccacttctc gatgccgagg aattcgcgga tgcgctccat
    18841 gtcgccgacc agcgcccggg tgtcgttgcc cccgagctcg gcatgcggcg tcgaatgccc
    18901 ggcgccacgc tggtcgaaca ggatgatgcg gtagtcctgc gggtcgaaga agcagcggtt
    18961 ggcgggctgg cagccg
//
LOCUS       DAORUQ010000040        18864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2552_length_18864_cov_6.753150, whole genome shotgun sequence.
ACCESSION   DAORUQ010000040 DAORUQ010000000
VERSION     DAORUQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2552_length_18864_cov_6.753150"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="nadA"
                     /locus_tag="PLW13_05540"
     CDS             <1..755
                     /gene="nadA"
                     /locus_tag="PLW13_05540"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HQY69868.1"
                     /translation="KRVLMPTLEATCSLDVGCPADLFGEFCDQHPDRTVVVYANTSAA
                     VKARSDWVVTSSIALDVVEYLSGRGEKILWAPDKYLGSWVQRQTGADMLLWEGSCIVH
                     EEFKAKGIEDLRKVYPDAAVLVHPESPEAVIALADAVGSTTQIIDAARRLPNPQFIVA
                     TDQGIFYQLQQQVPDKEFIIAPTAGEGATCRSCANCPWMAMNELQSLLACLEQGANEV
                     HVDPALGARAMLPLQRMLNFSRERALAVKGNA"
     gene            complement(855..2324)
                     /locus_tag="PLW13_05545"
     CDS             complement(855..2324)
                     /locus_tag="PLW13_05545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY69869.1"
                     /translation="MYLPLLRASSPRRALVAAGCIALAVKTGSARAVDIELPALGDAS
                     SAVVSPQAERRLGEAWLRMFRSQVRTVSDPLLTDYLEHVVFDLAEHSQLSDAQLKLVV
                     VENPTINAFAVPGGVMGIHNGLLLSAQHEDEMASVIAHELAHLSQRHFARGVEEARAN
                     AVPNMLALLGSLVVAATAGGNAGMAAITATQAAIQQQQLRFSRAHEREADRIGMLTLV
                     DAGYDADGMPRMFSRMLESLRYAGQRPPEFLLSHPVTESRVADSQNRARNYADQGRVD
                     SEDFGLMRARVQLGFDETPGFAIKRFRAEIEKDGGAAIGNVYGLALALTRAGEFDEAR
                     ATLAPLLQNHPDKIAFVIAAADIELASGKPEAAAHRLRRQLDVNPGNHPLTMAYANAL
                     LRTGRPKDAERVLEEHAQRKPEDPQLWYELAETHGLAGSIIDVHRARAEYFILNGALD
                     LAERQLGYALDLAQGDFHTTASVQARIADIREMREEIKL"
     gene            2487..2711
                     /locus_tag="PLW13_05550"
     CDS             2487..2711
                     /locus_tag="PLW13_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HQY69870.1"
                     /translation="MHEVDARGMSCPLPLLKAKKALNGMGPGERVRVLSTDPGSVRDF
                     RVFCEQSGNELLEFSCEDDIYTYVLRKRGD"
     gene            2734..3813
                     /locus_tag="PLW13_05555"
     CDS             2734..3813
                     /locus_tag="PLW13_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003345540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY69871.1"
                     /translation="MINTVRKWMDRYFAEEEAVILLLLPALGVLLIATLGDVLRPLIA
                     AAVLAFMIQGLVGWLARRGVPEPLALGLAFAVFTGIFLAFFLVLLPLVWNQLRSLVSD
                     LPGMLESLRQAVMQLRDAYPQFISEAQIRDLMERSSRDMAGLGQAALSFSLAYLPGVF
                     GLAIYVVIVPLLVFFFLKDRRQILAWCANLLPEERPRMSRIWHEMDEQLANYVRGKGV
                     EILIVGAVSIVAFSLFGLGYALLLGFLVGLSVVIPYVGAVVVTVPVAVVAWFQWGWTP
                     MFGYLMLTYLVIQILDGNVLVPLLFSEANNLHPVAIILAVLVFGSVWGVWGAFFAIPL
                     ATLVKAMMSAWPRSHAGAPAPQTPE"
     gene            3874..6774
                     /locus_tag="PLW13_05560"
     CDS             3874..6774
                     /locus_tag="PLW13_05560"
                     /inference="COORDINATES: protein motif:HMM:NF027513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQY69872.1"
                     /translation="MSAVPVPKSLVLMMLFALLAACAGPSPREANVIRGEQDGRAYRY
                     LELPNRMRVLLVSDPQTDKAAASLHVRAGSGNDPRSRQGLAHFLEHMLFLGTAKYPDP
                     GEYQEFISAHGGSHNAYTSVDHTNYFFDIEPGSLTPALDRFAQFFVAPLFNREYVERE
                     MNAVDSEYKLGLKDDARREYDVLRELVQPGHPLGSLAVGNLTTLGEGESDIREDLLAF
                     YATHYSANAMTLVVLGRESLDELQSMVDARFGAVADRSATELTQVPSMFAPGALPLEV
                     RIRPEQELRELTLLFPLPSARAHYAAKPLEYIGDLLGHEGPGSVLSVLKARGWAESLS
                     AGAGLDLYGEDAFQLSLQLTEEGVAHYRDIVALLFRAIDQMQEGGVEEWRFREQAKLG
                     DLAFRFREKGSPTGAVIALSNALQDYPVAEALRGPYLFRDYEPALIREYLGYLREDNL
                     LLTLSHRDVQTDRESRWFGTPYAVQKLDAGELARQAGAADGELALPVPNEFIPERVAL
                     KPLQGNAERPALLADEPGYRLWHLQDSAYPVPKASIYLQLLSPAANDNARDAALAALQ
                     ARLVSDALNEYAYPASLAGLQYSVESGARGLTVKIAGYDDKQRVLLAKVLQTLPRGRF
                     APDRFEGIKTEMLRDWANSAKQRPFLQLVDEMRASLVARSYRDEELRAALEPAGPEEL
                     ERFVRELFATGTLEILVHGNFSADEASGIARLAHETLGAVPPDALPPVEVVQLPPGAL
                     VRGINVDHPDSALLMYVQGAAGSDAERARVALSAQILGAPFFNELRTERQLGYVAFAQ
                     AYPLHRVPALVLAVQSPVAAPGALAREFEAFLQRQVAAAQALDEASFARQRQALVDRL
                     RESPKSLGERSERLWADAMLGAYGFDDRERVAAQVERIGREEWVEYFRAQIAGEARRA
                     MLLQSRGTAHAQVKEPPLPGRVLAPGDAWREAAGYFRFEREAIVPQAPAPAD"
     gene            6891..9545
                     /gene="aceE"
                     /locus_tag="PLW13_05565"
     CDS             6891..9545
                     /gene="aceE"
                     /locus_tag="PLW13_05565"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQY69873.1"
                     /translation="MQEDIDSAETGEWLESLEAVVRNRGKARAAYLLKRLADHATGAG
                     VRLPPAITTRYRNTIAPEAEKRMPGDLFVDRRIRSLIRWNALAMVMRANDDDEGLGGH
                     IATFLSSATLYDVGFNYFFRGNSPGKPGDLVFFQGHSAPGMYARSYLEGRLSEEQLDN
                     FRREVDGRGLSSYPHPWLMPDYWQFPTVSMGLGPIQAIYQAHVMRYQQNRGLVDHGDR
                     KVWAFLGDGECDEPEALGAIGLAGREQLDNLIFVVNCNLQRLDGPVRGNGKIIQELEG
                     VFRGAGWNVIKVIWGRLWDPLFARDESGRLQQRMDEVCDGEYQNYKFNGGAYTREHFF
                     GKYPETRELVQDLSDDDIMYLNRGGHDPYKVYAAYHEAFHHKGQPTVILAKTVKGYGM
                     GASGEAANETHSVKKMDLESLKKFRTRFGIPLRDDELKSVPYYRPAPDSPEMVYMRQR
                     REALGGNLPSRLVTDEQLPVPALDAFAAQLKGSAHREISTTMAFVRLLSTLVKDPGMG
                     DRVVPIVPDEARTFGMEGMFRQLGIYSSVGQRYTPHDAAQILFYKEDHKGQILEEGIN
                     EAGAMSAWIAAGTSYANHNLTQIPFYIFYSMFGFQRIGDLAWAAGDQQARGFLIGATS
                     GRTTLNGEGLQHQDGHSHLLAATIPNCVSYDPTYAYEVAVIVHHGLEVMYGRRESKFF
                     YITTMNENYQHPEMPAGAEEGIIRGMHRVESAGPKAKTVVQLMGCGSILREVRAAAAL
                     LREDFGIDADVWSLTSINELRRDGLDAERWNLLHPTAKAPRVPYITQMLDGATGPVIA
                     STDYTRAWADGLRPFIRQRYAVLGTDGFGRSDTRARLRHFFEVDRHFVAVAALKALAD
                     EGRVKPAKVAEAIAKYGIDPAKPNPANV"
     gene            9585..11270
                     /locus_tag="PLW13_05570"
     CDS             9585..11270
                     /locus_tag="PLW13_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HQY69874.1"
                     /translation="MKRDSVKVPELGGASEVEVIEIAVADGAAVATGQTLITLESDKA
                     SMEVPSPLAGTVRGISVKVGDRVREGDEILVMEIAGEAAAASAPPVMSASTVTAAAVP
                     APVAPGKAPASGGAVLALRVPDLGGADSVEVIEISARAGDSVTEGASLLVLEGDKASM
                     EFPAPADGTVLTLDIKVGDRVTSGQKLGELRTAAAAAAAPAPAPVEQQAAAVAAPAAA
                     DVAAPAPAAAPAPAAQATLAEGDAVAAITRVFEKETRLAAGRAPSAAAAPVYAGPAVR
                     QLAREIGITLGEVSGSGPKGRILREDLRAYVKARLAAPPAASLPTVPDVDFAAFGPIE
                     TVALSNIQKATVTNMSRSWLNVPHVTQFDYADVTELEAFRDSLKPEMEQRKNKLTPLP
                     FLLLAVARALREHPRFNAALAPDGANLVLKKYFHVGIAVDTPQGLVVPVIRDVDRKSV
                     WELAAEARELAEKARNRKLKLAEMQGACFTISSLGNIGGEGFTPIVNAPEVAILGVSK
                     LAVRPVWNGQEFLARKTLPLSLSYDHRVINGADAGAFLTWLVGALGDMRRLLL"
     gene            11597..13300
                     /locus_tag="PLW13_05575"
     CDS             11597..13300
                     /locus_tag="PLW13_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69875.1"
                     /translation="MNIRKGLSAAGIVGRCGAILALLLIAQLAAADIGDTTVTFTPSG
                     VGVLGTPTYVWGPDEINHDPTTGGSQALAVDLGADVVWAEVLISTLRSTGNEVGAQRE
                     RGFWQAFDGYGLEVGNGEFDGTTLEGYSGDVGTLVIENVGNFRYLVFTALPYLTPLPN
                     ESDSSDYHVRGIRVGYPDLSTASFGGLNAGQAGWVGVGLYGFDNGSEFTEGGRFIGAA
                     PAPIVVNDQECSTGQGTNNQSCKIQFTQELTTTIAGEDVEGIIKVAGISTVVDFRSGC
                     GYSGSPPGQKLPGASKPIEEINGALDLGFALDPLHSDVTGPIVPSHLCGIPPTQGASN
                     GEFVLVNLDSDIVISRSVIEHEVKNDPHIDTGYECATGAAVLKDANPSERRSRLPVFG
                     WLPKTSNPEIPVLDAYRELVPVLEDVTTGCGSTRSGASRLSFLVYDLHHAPAADYRSI
                     IRDEILQLQATVNETPVCVDSSQAENLKSRVRLTLNGYDNNRLSYAKKELGGLLSLVQ
                     SKRLDREFAKCAFDLDSWMVTPVNPTDQDLVPRNFRGDLIVQIRHIQYMMDRMLGVTS
                     P"
     gene            13527..15773
                     /locus_tag="PLW13_05580"
     CDS             13527..15773
                     /locus_tag="PLW13_05580"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HQY69876.1"
                     /translation="MGPAEASAYKYKAFISYSHRDEKWARWLHRALETYRVPRHLVGQ
                     QTSMGVIPVRPSPVFRDRDELPSATDLGAKLREALEGSACQIVICSPASAASHWVNEE
                     ILSFKRLGRAERIFALIVGGEPYASGMPGRESEECFPPALRYQLDLDGKLSDVPAEPI
                     AADARPGKDGKSNAQIKLLAGMLGVGFDALRQRELQRRQRRLVMLTSAALTGMVVAIG
                     LATSAVIARNEAQLQRERAEKEAETARKTSAFMVGLFKVADPSQARGQITAVELLQVG
                     ARRIDTELKGQPGVQATLMDTIGTVYTGLGLYNDAHTMLERALAQRRALAPVDPREIA
                     RSQVHLAQVLTEKAELEAAERLFNEAIAVLQGSASEFVSADELAAALAGLAEVYFRAG
                     RYPEAEPLLARVYEIRRARLPPGDPAIADALEELGLNQFDQGNYERAEIYLRDALAMR
                     RVSLGDAPHPDVAENLNNLALLLMESSRFEESERLFQEAMTMNSALYGAVHPDVAAGL
                     NNLGLLYRSQGDLAKAEESYSNALGMQRQLRGEKHPEVARVQSNLAFVYYDEGQLARA
                     IDTMREAMEMQRATSGPRHPDTANSMSALGRWLSEAGKDAEAAVLLRQALALLTELHG
                     ADHPDVAIAELGLAQTLARTGPLDEALAMAKSAAAKVDEAFGPEHWIAAVAGSTEGSV
                     LAAMGRPAEAEPLLKQGYEALRDNPSARPAYLRRARESLVAFYEESGRKDLALTYRQE
                     ASAVAPGS"
     gene            complement(15817..16248)
                     /locus_tag="PLW13_05585"
     CDS             complement(15817..16248)
                     /locus_tag="PLW13_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGDYG domain-containing protein"
                     /protein_id="HQY69877.1"
                     /translation="MNTAATLAGQPGARRARKLPEEVQVRFARTPGICRTLEGDVRYD
                     AGDAIVEARAGDRWPVRREVFRQRYEPVAPTPAMHDGRYRKRGIEVLARQLTDTARVL
                     LPGGRGELTGKPGDWLVEYGADDLAIVGRDIFATTYELIAD"
     gene            complement(16245..18059)
                     /locus_tag="PLW13_05590"
     CDS             complement(16245..18059)
                     /locus_tag="PLW13_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69878.1"
                     /translation="MHAIESAIDRLVAHIAAQFAELADCEQLYGAQAPRRCVTRIIGG
                     GYTGTDEMAARIAARRGCPLHRVMTAAEAQQSGLQAEGPDRRDLVLGEANDAGASSVT
                     INLRDETALAFSDLLMVVWDGHDPRGPAGGTVRLLREAVLCSKPVIWIDLAGTARLLD
                     ISRIDEPTLFLLGADDSIAELLAPLFVTLDEQALDVELALIANPLAPQAAGRVSPALC
                     DRVRRYFTQRAGSRFSERRAGIVSELLKVTLGGSLAPASLLTGIRKVFARDATRPWFG
                     VAAEHGADGAAASALPVEPAGIEDRFNWSDYQANLAAGIRRDVTLFLYAFATLAVFAA
                     VAGNIKLGVSDHSSFWTWVELGMIMFICVLVMLASRRRWHGEWLSHRLVAEELRCLRL
                     GYPFVAAPESCTQVPRRSDAQASGRAQWVLDGAEQWALRRSAVASGLPRMPDGSAFVA
                     HERFSESKQYLLDVLNGQADYHRRNAETHHRMSHRLHHTTGIAFLLTGCAVVAHFFLH
                     ADWLLLFTAALPALASGIHGLVSSNEMERVATMSEEAGIRLRHVVRGIERLEQRSDSR
                     WIAWLRLRQLACEGTAAMSEVNGQWQDLLKHRETTLPA"
     gene            complement(18276..>18864)
                     /locus_tag="PLW13_05595"
     CDS             complement(18276..>18864)
                     /locus_tag="PLW13_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQY69879.1"
                     /translation="MMFNNSGNIGIPLAVLAWGETALPAAVILFMVENLLHFSLGARM
                     LDANARLLTLWRIPAVGATFAGLAVSLLGLQVWGPLLSALQMLGDIAIPLLLFALGVR
                     MKTIRFTDIGAPLVGAFLRPALGVAIAWCAAQLLGLGARDTAMLIVFGALPPAVLNFL
                     FAERYRQEPERVASIVLLGNLAAVLVLPLVLAAVL"
BASE COUNT         3047 a         6311 c         6508 g         2998 t
ORIGIN      
        1 aaaagcgcgt gctgatgccc acgctcgagg cgacctgctc gctcgacgtg ggttgccccg
       61 cggacctgtt cggcgaattc tgcgaccagc accccgaccg caccgtcgtt gtctatgcca
      121 acacctcggc cgccgtgaag gcgcgctccg actgggtcgt gacctcgagc atcgcgctcg
      181 acgtggtcga gtacctcagc ggtcgcggcg aaaagatcct gtgggcgccc gacaagtacc
      241 tgggttcctg ggtgcagcgc cagacgggtg ccgacatgct gctctgggag ggcagctgca
      301 tcgtgcacga ggagttcaag gccaagggca tcgaggacct gcgcaaggtc tacccggatg
      361 ccgccgtgct ggtgcacccg gagtcgcccg aggccgtgat cgcgctggcc gacgccgtcg
      421 gcagcaccac gcagatcatc gacgcggcgc gccgcttgcc gaacccgcaa ttcatcgtcg
      481 ccacggacca gggcatcttc taccagttgc agcagcaggt gcccgacaag gagttcatca
      541 tcgcgcctac cgccggcgag ggtgcgacct gtcgcagctg cgcgaattgc ccgtggatgg
      601 cgatgaacga actgcagagc ctgctcgctt gcctcgagca gggcgcgaac gaagtccacg
      661 tcgatcccgc gctcggcgcg agggcaatgc tgccgctgca gcgcatgctg aatttctcgc
      721 gcgagcgcgc gctcgcggtg aagggcaacg cctgatccgt gggtccggct tcggccggac
      781 aatgacccga tccgtgggtt cggcttcagc cggacacggg ctgtccgaat gaattcggac
      841 ccacgagtcc gggcctacag cttgatctct tcgcgcatct cgcggatgtc cgcgatgcgc
      901 gcctgcacgc tcgcggtggt gtggaaatcg ccctgggcga gatcgagggc gtagcctagc
      961 tggcgctcgg cgaggtccag cgccccgttc aggatgaagt actccgctcg tgcccggtgc
     1021 acatcgatga tgctcccggc gagcccgtgg gtctcggcca gctcatacca cagctgtggg
     1081 tcctcgggct tgcgctgcgc atgctcctcc agcacgcgtt ccgcgtcctt cggcctgccg
     1141 gtgcgcagca gcgcattcgc ataggccatc gtcagcggat ggttgcctgg attgacgtcc
     1201 agctgccggc gcaggcgatg cgctgccgct tcgggcttgc cggaggccag ctcgatgtcc
     1261 gcggcggcga tgacgaacgc gatcttgtcc ggatggttct gcagcagcgg cgccagcgtt
     1321 gctcgcgctt cgtcgaactc gcccgccctg gtcagtgcca gtgccagccc gtagacattg
     1381 ccgatcgcgg cgccgccatc cttctcgatc tcggcgcgaa agcgcttgat cgcaaagccg
     1441 ggagtctcgt cgaaaccgag ctggacgcga gcgcgcatca gaccgaaatc ctcgctgtcc
     1501 acgcgccctt ggtcggcgta gttgcgcgcg cggttctgcg agtcggcgac gcggctctcc
     1561 gtgaccgggt gcgacagcag gaattccggc gggcgctgcc ccgcatagcg cagcgattcg
     1621 agcatgcgcg agaacatccg cggcatgccg tcggcgtcat agcccgcatc caccagcgtc
     1681 agcatcccga tgcggtccgc ctcgcgttcg tgcgcgcggc tgaagcgcag ctgctgctgc
     1741 tggatggcgg cctgggtcgc ggtgatggcg gccatgcccg cattgcctcc agcggtggcg
     1801 gcgacgacaa ggcttcccag cagcgcgagc atgttgggca cggcgttggc gcgcgcctcc
     1861 tcgacgccac gcgcgaagtg tcgctggctg aggtgggcca gttcgtgcgc gatcacggag
     1921 gccatctcgt cctcgtgctg ggcagagagc agcaagccgt tgtggatgcc catgacgccg
     1981 ccgggcaccg cgaaggcgtt gatcgtcgga ttctcgacca ccacgagctt cagttgcgcg
     2041 tcgctcagct ggctgtgctc cgcgagatcg aaaacgacat gctcgaggta atccgtcagc
     2101 agcggatccg atacggtgcg gacctggctg cggaacatgc gcagccacgc ttccccgagc
     2161 ctgcgctcgg cctgcggcga gaccacggcg gaagatgcgt caccgagcgc gggaagctcg
     2221 atgtccacgg cgcgtgcact gccggtcttc acggcaagcg cgatacagcc ggcggcaacg
     2281 agggcgcggc gcggcgagct cgcgcggagc aggggcaaat acacgtgagg caacgtcctg
     2341 tgatgatgcg tccagagacg ccccgtttga tcgcgcgcgc ggcaaataat tccccgcggt
     2401 tgcctgcgcg ccttggggcg cccggtgccg gtggctatac tagcacgccg tcgcatgcgc
     2461 gacggcccgg gccggggagt ggatcgatgc acgaggtgga tgcgcgcggg atgtcctgtc
     2521 cgctgcccct gctgaaggcc aagaaggcgc tgaacggcat ggggcccgga gagcgcgtgc
     2581 gcgtgctcag taccgatccc ggctccgtgc gggatttccg tgtcttctgc gagcagagtg
     2641 gcaacgagct gctcgaattc tcctgcgagg atgacatcta cacctacgtg ctgcgcaagc
     2701 gtggcgactg aaggggcatc gggcgggcgc gtaatgatca ataccgtgcg caagtggatg
     2761 gatcgctatt tcgccgagga agaggcggtg atcctgctgt tgctgcccgc actgggcgtg
     2821 ctcctgatcg cgacgctcgg cgacgtgctt aggcccttga tcgccgcagc ggtgctggcc
     2881 ttcatgatcc agggcctggt cgggtggctg gcgcgccgcg gcgtgcccga gccgctggcc
     2941 ctcggcctgg cctttgccgt gttcacgggc atcttcctcg cgttcttcct ggtgctgctg
     3001 ccgctggtct ggaaccagct gcgcagcctg gtgagcgatc ttcccggcat gctcgagtcg
     3061 ctgcgccagg cggtgatgca gttgcgcgac gcctatccgc agttcatcag cgaggcgcag
     3121 atacgggact tgatggagcg cagcagccgg gacatggccg gtctcggtca ggccgccctg
     3181 tcgttctcgc tggcgtacct gccgggcgtg ttcgggctgg cgatctacgt cgtcatcgtg
     3241 ccgctgctgg tgttcttctt cctcaaggac cggcgccaga tcctggcgtg gtgcgcgaac
     3301 ctgctgccgg aggagcggcc gcggatgtcg cgcatctggc acgagatgga cgagcagctg
     3361 gcgaactacg tgcgcggcaa gggcgtcgag atcctgatcg tcggcgccgt gtcgatcgtc
     3421 gccttctcgc tgttcggcct gggctatgcg ttgctcctgg ggttcctggt cgggctgtcg
     3481 gtcgtgattc cctacgtggg cgcggtggtg gtcacggtgc cggtggcggt ggtggcctgg
     3541 ttccagtggg gctggacccc gatgttcggc tacctgatgc tcacctacct cgtgatccag
     3601 atcctcgacg gcaatgtact cgtgccgctg ctgttctccg aggcgaacaa cctgcacccg
     3661 gtcgcgatca tcctcgcggt gctcgtgttc ggttctgtct ggggcgtgtg gggggccttt
     3721 ttcgccatcc cgctcgccac gctcgtcaag gccatgatga gcgcctggcc gcgctcgcac
     3781 gcgggcgcgc cggcgccgca aacgccggag tgaaactgcc gcgtgttccg gtggtctgac
     3841 ccaaacatcc gcaattcgtc tcctggagtc gcaatgtccg cagtccctgt gccgaaatcc
     3901 ctcgtcctga tgatgctgtt cgcgttgctg gcggcctgcg cgggcccgtc gccacgcgag
     3961 gcgaatgtta tccggggcga gcaggacggc cgtgcatacc gttacctcga gctgccgaac
     4021 cgcatgcgcg tgctgctggt ctcggatccg cagaccgaca aggcggccgc cagcctgcat
     4081 gtgcgcgccg gcagcggcaa cgacccgcgc tcgcgccagg ggctggcgca cttcctggag
     4141 cacatgctgt tcctggggac cgcgaagtat cccgatcccg gcgaatacca ggagttcatc
     4201 agcgcgcacg gtggctcgca caacgcctac acctcggtcg accacacaaa ctacttcttc
     4261 gacatcgagc cgggcagcct cacgcccgcg ctggaccggt tcgcgcaatt cttcgtggcg
     4321 ccgctgttca accgcgagta tgtcgagcgc gagatgaacg cggtcgattc cgagtacaag
     4381 ctcggtctca aggacgacgc gcggcgcgaa tacgacgtgc tgcgcgagct ggtgcagccc
     4441 ggacatccgc tgggctcgct cgcggtgggc aacctgacaa cgctcggcga aggcgagagc
     4501 gacatccgcg aggacctgct cgcgttctac gccacgcact attccgcgaa cgcgatgacc
     4561 ctcgtggtgc tggggcgcga gtcgctggac gagctccagt cgatggtcga cgcgcgtttc
     4621 ggtgccgtgg cggaccgttc ggccaccgag ctgacgcagg tgccgtcgat gttcgccccc
     4681 ggcgccctgc cgctggaggt gcgcatccgc cccgagcagg agctgcgcga gctcacgctg
     4741 ctgttcccgc tgccctcggc gcgcgcgcat tacgccgcca agccgctcga atacatcggc
     4801 gacctgctgg gtcacgaggg tccgggcagc gtgctgtcgg tgctgaaggc gcgcggctgg
     4861 gccgagagcc tgagcgccgg cgcggggctc gacctgtatg gcgaggatgc cttccagctc
     4921 agcctgcagc tcacggagga gggtgtggcg cattaccgcg acatcgttgc gctgttgttt
     4981 cgagccatcg accagatgca ggagggcggc gtcgaggagt ggcgattccg cgagcaggcg
     5041 aagctcggcg acctggcatt ccgcttccgc gagaaaggca gccccaccgg cgcggtgatc
     5101 gcgctgtcca acgcgttgca ggactacccg gtggccgagg cgctgcgcgg cccctacctg
     5161 ttccgcgatt acgagcccgc gctgatccgc gagtacctgg gctacctgcg tgaggacaac
     5221 ctgctgctga ccctgtcgca ccgcgacgtg cagaccgacc gcgagagccg ctggttcggc
     5281 acgccctacg ccgtgcagaa actcgatgcc ggcgagctgg cgcgccaggc cggtgccgcg
     5341 gacggcgagc tggcgctgcc ggtgccgaac gagttcattc ccgagcgcgt ggcgctgaag
     5401 ccgctgcagg gcaacgccga gcggccggct ctgctggccg acgagcccgg ctaccggctg
     5461 tggcacctgc aggacagcgc ctacccggtg cccaaggcct cgatttacct gcagctgctc
     5521 tcgccggcgg cgaacgacaa cgcccgcgac gccgcgctcg cggcgctgca ggcgcgcctg
     5581 gtgagcgacg cgctcaacga gtacgcctac ccggcgagcc tcgcgggcct gcagtacagc
     5641 gtcgagtcgg gcgcgcgcgg actcaccgtg aagattgccg gctacgacga caagcagcgc
     5701 gtgctgctcg ccaaggtgct gcagaccctg ccccggggcc gtttcgcgcc cgatcgtttc
     5761 gagggcatca agaccgagat gctgcgcgac tgggccaaca gcgccaagca gcgccccttc
     5821 ctgcaactgg tggacgagat gcgcgcgagc ctcgtggcgc gcagctaccg cgacgaggag
     5881 ctgcgggcgg cgctggagcc tgcgggtccc gaagaactgg agcgtttcgt gcgcgagctg
     5941 ttcgccacgg gcacgctgga gatcctggtg cacggaaact tcagcgccga cgaggcgagc
     6001 gggatcgcgc ggctcgcgca cgagacgctt ggcgcggtgc ctccggatgc gctgccgccg
     6061 gtcgaggtcg tgcagcttcc ccccggtgcg ctggtgcgcg gcatcaacgt cgatcacccg
     6121 gattcggcgc tgctgatgta cgtccagggc gccgcgggtt ccgatgccga gcgtgcccgc
     6181 gtggccctca gcgcgcagat actcggtgcg cccttcttca acgagctgcg caccgaaagg
     6241 cagctcggtt acgtggcctt cgcccaggcc tatccgctgc accgcgtgcc cgcgttggtg
     6301 ctcgcggtgc aatcgccggt ggctgcgccc ggggcgctgg cacgggagtt cgaagccttc
     6361 ctgcagcggc aggtcgccgc ggcgcaggcg ctcgacgagg cgtcgttcgc acgccagcgc
     6421 caggcgctgg tcgatcggct gcgcgagtcg ccgaagtccc tcggcgaacg cagcgagcgg
     6481 ctgtgggcgg acgcgatgct cggtgcctac ggctttgatg accgcgagcg cgtcgccgcg
     6541 caggtcgagc gcatcggtcg cgaggagtgg gtggagtatt ttcgcgcgca gatcgcgggc
     6601 gaggcgcggc gcgcgatgct gctgcagagc cgcggcacgg cgcatgcgca agtgaaggaa
     6661 ccaccattgc cgggacgcgt gctcgccccc ggtgacgcgt ggcgcgaagc cgccgggtat
     6721 tttcgcttcg aacgcgaggc gatagtgccg caagcgcccg cgccggcgga ctagcgccag
     6781 ggcgcgaggc gccgcgcata ccgctgtggt cgccggagcg gctaaactgg cgccaatccg
     6841 aaaccgccgg ggcagtacac tgtcccgcca gcccacgcac aggagtattc atgcaggagg
     6901 atatcgattc cgcggaaacc ggcgaatggc tggaatcgct ggaagccgtg gtgcgcaatc
     6961 gcggcaaggc gcgcgcggct tacctgctga aacggctcgc cgaccacgcc accggtgccg
     7021 gcgtgcgcct gccgccggca atcaccacgc gttatcgcaa caccattgcg cccgaggcgg
     7081 aaaagcgcat gcccggcgac ctcttcgtcg accggcgcat ccgctcgctg atccgctgga
     7141 acgcgcttgc gatggtgatg cgcgccaacg acgatgacga gggtctcggc ggccacatcg
     7201 cgaccttcct ctcgagcgcc acgctctacg acgtgggctt caactatttc ttccgcggca
     7261 acagccccgg caagccgggt gacctggtgt tcttccaggg gcacagcgcg cccgggatgt
     7321 atgcgcgctc gtatctcgag gggcggctca gcgaggagca gctcgacaat ttccgccgcg
     7381 aagtcgacgg gcgcgggttg tcgtcctacc cgcacccgtg gctgatgccc gattactggc
     7441 agttcccgac ggtgtcgatg ggcctcggcc cgatccaggc catctaccag gcgcacgtga
     7501 tgcgctacca gcagaaccgc ggactggtcg atcacggcga ccgcaaggtc tgggccttcc
     7561 tgggcgacgg cgaatgcgac gagcccgagg cactgggagc catcggcctc gccgggcgcg
     7621 agcagctgga caacctgatt ttcgtcgtca actgcaacct gcagcgcctc gacgggccgg
     7681 tgcgcggcaa cggcaagatc atccaggagc tcgagggcgt gttccgcggc gcgggctgga
     7741 acgtgatcaa ggtgatctgg ggccggctgt gggacccgct gttcgcgcgc gacgagtcgg
     7801 ggcggctgca gcagcgcatg gacgaagtct gcgacggcga gtaccagaac tacaaattca
     7861 acggtggcgc ctacacgcgc gagcatttct tcggcaagta tcccgagacg cgcgagctgg
     7921 tacaggatct ttccgacgac gacatcatgt atctcaatcg tggcggccac gatccctaca
     7981 aggtctacgc ggcctaccac gaggcgtttc accacaaggg ccagcccacg gtgatcctcg
     8041 ccaagaccgt gaagggttac ggcatgggcg cctcgggcga ggcagccaac gagacccact
     8101 ccgtcaagaa gatggatctc gagagcctga agaaattccg cacccgcttc ggcatccccc
     8161 tgcgcgacga cgagctgaag tccgtgccgt actaccgccc ggcgcccgac agtcccgaga
     8221 tggtctatat gcgccagcgc cgcgaggcgc tgggcggcaa cctgcccagc cgcctcgtca
     8281 ccgacgagca gttgccggtg ccggcgctcg atgcctttgc ggcgcagctc aagggcagcg
     8341 cgcaccgcga gatctctacc acgatggcct tcgtgcgcct gctgtcgacg ctggtgaagg
     8401 atcccggcat gggcgatcgc gtcgttccca ttgtgcccga cgaggcgcgc accttcggca
     8461 tggaggggat gttccgccag ctcggcatct attcatcggt ggggcagcgc tacacgccgc
     8521 acgacgcggc acagatcctg ttctacaagg aagaccacaa ggggcagatc ctggaggagg
     8581 gtatcaacga ggccggcgcg atgtcggcgt ggatcgccgc gggcacttcc tatgccaacc
     8641 acaacctcac ccagatcccc ttctacatct tctactcgat gttcggcttc cagcgcatcg
     8701 gcgacctcgc ctgggcggcc ggcgaccagc aggcacgcgg cttcctgatc ggcgcgacct
     8761 cggggcgcac cacgctgaac ggcgaaggcc tgcagcacca ggacggccac agccacctgc
     8821 tggccgcgac gattcccaac tgcgtcagct acgacccgac ctacgcctac gaagttgccg
     8881 tgatcgtgca ccacgggctc gaggtgatgt acggccggcg cgagagcaag ttcttctaca
     8941 tcaccacgat gaacgagaac taccagcacc ccgagatgcc ggcgggcgcg gaggagggca
     9001 tcatccgcgg catgcaccgc gtcgagagcg cggggccgaa ggcgaagacc gtggtgcagc
     9061 tgatgggctg cggctcgatc ctgcgcgagg tgcgggccgc cgcggcgctg ctgcgcgagg
     9121 acttcggcat cgacgccgac gtgtggagtc tcaccagcat caacgagctg cgccgtgacg
     9181 gcctcgacgc cgagcgctgg aacctgctgc accccacggc aaaggcgccg cgggtgccgt
     9241 atatcacgca gatgctcgat ggcgcgacgg ggccggtgat cgcgtccacc gactacacca
     9301 gggcctgggc cgacgggctg cggcccttca tccggcagcg ctacgccgtg ctgggcacgg
     9361 acggcttcgg gcgcagcgac acgcgcgctc gcctgcgcca tttcttcgag gtggaccgcc
     9421 acttcgtggc cgtggcggcg ctgaaggcgc tggccgacga ggggcgtgtc aagcccgcga
     9481 aggtggctga agcgattgcg aaatacggca tcgatcccgc aaagccgaac ccggcgaacg
     9541 tgtgaaccgg tgacgcaagc ccagccctgg acaaaggaaa cagcgtgaag cgtgacagcg
     9601 tgaaggtccc cgaactcggc ggggcgagcg aagtggaagt gatcgagatc gcggtcgccg
     9661 acggtgccgc ggtcgccacc gggcagaccc tgatcaccct ggagtccgac aaggcctcga
     9721 tggaggtgcc ctcaccgctc gcgggtaccg tgcgcggcat cagcgtgaaa gtcggcgatc
     9781 gcgtgcgcga aggcgacgag atcctggtga tggagatcgc gggtgaggcc gccgcggcgt
     9841 ccgccccgcc ggtgatgtcc gcgtccaccg taacggctgc cgcagtacct gctcccgtcg
     9901 cgcccgggaa ggcgccggca agcggcggcg ccgtgctggc gctgcgggtg ccggatctcg
     9961 gcggtgccga cagcgtggag gtcatcgaga tctcggcgcg tgcgggcgac agcgtgacgg
    10021 aaggcgcgtc gctgctggtg ctggagggcg acaaggcctc gatggaattc ccggcccccg
    10081 ccgatggcac cgtgctgacg ctcgacatca aggtgggcga cagggtgacg agcgggcaga
    10141 aactcggcga gctgcgcacg gccgcggcgg cggctgccgc tcccgctccc gctcccgtgg
    10201 agcaacaggc ggctgccgtc gcagcgcccg cggccgccga tgtggctgcg ccagcacctg
    10261 cagcagcgcc agcgcctgcc gcgcaggcca cgctcgccga aggcgacgcg gtggccgcga
    10321 tcacgcgcgt gttcgagaag gagacccggc tggcggccgg gcgcgcgccg agcgcagcgg
    10381 cagcaccggt ctatgccggg cctgcggtgc gccagctggc gcgcgagatc ggtatcacgc
    10441 tgggcgaggt gagcggcagc ggccccaagg gacgcattct ccgggaagac ctgcgcgcct
    10501 acgtgaaggc gcgcctggcg gcgccgccgg cagcctcgct cccgacggtg ccggacgtgg
    10561 atttcgcggc tttcggcccg atcgagacgg ttgcgctgag caatatccag aaagccaccg
    10621 tgaccaatat gtcgcgcagc tggctgaacg tgccgcacgt cacgcagttc gattacgccg
    10681 acgtcaccga gctcgaggct ttccgcgaca gcctgaagcc cgagatggag cagcgcaaga
    10741 acaagctcac gccgctgcct ttcctgctgc tggccgtggc gcgcgcgctg cgcgagcacc
    10801 cgcgcttcaa tgccgcgctg gcgcccgacg gcgcgaacct ggtgctgaag aagtatttcc
    10861 atgtcggcat cgcggtcgac acgccgcagg gcctcgtggt gcccgtgatc cgcgacgtcg
    10921 accgcaagtc ggtgtgggag ctggccgccg aggcgcgcga actggccgag aaggcgcgca
    10981 accgcaagct gaagctcgcc gagatgcagg gcgcctgctt cacgatctcg agcctcggca
    11041 acatcggcgg cgagggcttc acgccgatcg tgaatgcgcc ggaagtcgcg atcctcggcg
    11101 tatcgaaact cgcggtgcga ccggtgtgga acgggcagga attcctggcg cgcaagacgc
    11161 tgccgctgtc gctgtcctac gaccatcgcg tgatcaacgg cgcggacgcg ggcgctttcc
    11221 tcacctggct ggtcggcgcg ctgggcgata tgcggcgcct gctgctgtag atcgcttgcg
    11281 cgccgccggc gggcgcgatt ccgtcaggtt tttttacagt gccggggtat ccgtagcacg
    11341 ccctcccgcg attgtgcccg cgcgttcaag tggtgcgacc tgctcgcgtg gcgcatcggt
    11401 gtggtgcgtt tcgcaaccgt ttgcgcttgt ttgccagcct cttcaacgaa tgaacggcaa
    11461 ttaatgttgc cctgcttgca ccgtcgtgta tcgctggagc atgatgccgg ccttgacata
    11521 acgcaaaaac gagctagtgg cgcgatcttt gcagctgttt gtcagttgat cggtgtccca
    11581 aggcgaggca gtcagcatga acattcgaaa agggctatcg gccgcaggca tcgtgggtcg
    11641 ttgcggtgcg atcctcgcgt tgctgctcat cgcgcagctc gcggcggcgg atatcggcga
    11701 caccacggtc actttcacac cctccggcgt aggcgtgctt ggtaccccga cctacgtgtg
    11761 gggacccgat gagatcaatc acgaccccac gactggcgga agtcaggctc tcgccgtcga
    11821 tctcggcgca gacgtggtct gggcagaggt cctcatctct accctgcgca gcactggaaa
    11881 tgaagttggg gcacagcgtg agcgcggttt ctggcaggcc tttgacggct acggtctgga
    11941 ggttggcaac ggggagttcg atggcaccac tctcgaaggc tattccggag acgtcggaac
    12001 gctggtgatc gagaacgtcg gaaatttccg ctacctcgta ttcaccgcgc tgccctacct
    12061 cactccgttg ccgaatgaga gcgacagttc cgattaccat gtccgcggca tcagggtcgg
    12121 ttatcccgac ctgagcactg cctcgttcgg agggctcaac gctggccagg cgggttgggt
    12181 cggtgtgggc ctgtacggat tcgacaacgg gtctgaattc acggagggcg ggcgcttcat
    12241 cggcgcagcg ccagcgccga tcgtcgtcaa tgaccaggag tgcagcaccg gccaggggac
    12301 gaacaaccag agttgcaaga tccagttcac ccaggaactc accaccacca tcgccgggga
    12361 agacgtcgaa gggatcatca aggtggcggg tatctcgact gtcgtcgatt tccgctccgg
    12421 ctgcggatac agcggtagcc ccccggggca gaagctgcca ggtgccagta agccgatcga
    12481 ggaaatcaat ggcgccctgg atctcggttt cgcgctcgat ccgctccact ccgatgtgac
    12541 cgggcctatc gtgccgtccc atctgtgcgg cattcccccg acgcagggtg cgtcgaacgg
    12601 cgagttcgta ctggtcaacc tggactccga catcgtgatt tcgcgttcgg tgatcgagca
    12661 cgaggtcaag aacgaccctc acatcgatac cgggtatgag tgcgcgaccg gtgcggcggt
    12721 gttgaaggac gccaacccga gcgagcggcg cagccgtctg ccggtgttcg gctggctgcc
    12781 gaagacctcg aacccggaga ttcccgtgct cgacgcctac cgggaactcg tccccgtgct
    12841 cgaggacgtc accaccggct gcggcagcac ccgctcgggc gccagcaggc tgtccttcct
    12901 cgtctatgat ctgcaccatg ccccggcagc cgactaccgc agcatcattc gcgacgagat
    12961 tttgcaactg caggccaccg ttaacgaaac gccggtctgc gtggattcgt cccaggcgga
    13021 aaacctgaaa tcgcgggtca ggctgaccct caacggctac gacaacaacc ggctcagtta
    13081 tgccaagaag gaactgggcg ggctgctgag cctggtgcag tccaagcgac tcgaccgtga
    13141 gttcgcgaag tgcgccttcg acctcgactc gtggatggtg accccggtga acccgaccga
    13201 tcaggatctg gttccccgca acttccgcgg cgacctgatc gtgcagatcc ggcacatcca
    13261 gtacatgatg gaccggatgc tcggcgtcac cagcccctag ctgccagagg gtatctgcgc
    13321 gcaccgttcc gtgaccgcgt cggcgggagg aggtctcctc ccgccggatg tcgggccact
    13381 cggtccttcc ttgcggcaag cacgcctcga tgcgcctcgc gctgctctgg tggccccggt
    13441 agcctgcggg gcccgccctc cggtagagtg gcttcgttgc cccccgttgc gggcgagcat
    13501 cagggttcga cctgaaggaa acgcgtttgg gcccggcaga ggcatccgcc tacaagtaca
    13561 aggctttcat cagctacagc caccgcgacg agaaatgggc gcggtggctg catcgtgccc
    13621 tcgaaaccta tcgcgtaccc aggcacctgg tcgggcagca gacgtccatg ggcgtcatcc
    13681 cggtgcgtcc ctcaccggtt tttcgcgacc gtgacgaact tcccagcgcc acggatctgg
    13741 gtgcgaaact gcgcgaggcg ctggagggtt cggcctgcca gatcgtgatc tgctcgccgg
    13801 cgtcggcggc ttcgcactgg gtcaacgagg agatactttc cttcaagcgg ctgggccgcg
    13861 ctgagcgcat tttcgccctg atcgtgggcg gtgaaccgta cgcctcggga atgcccgggc
    13921 gcgaatccga ggaatgcttc ccgccggcgc ttcgctatca actggacctg gatggcaagc
    13981 tctcggacgt gccggcggaa cccattgccg cggacgcgcg cccgggcaag gacggcaagt
    14041 ccaacgcgca gatcaagctg ctcgccggca tgctcggcgt aggcttcgac gcgttgcgcc
    14101 agcgcgaact gcagcggcgc cagcgccggc tggtgatgct caccagcgcc gcgctgaccg
    14161 gcatggtcgt cgcgatcggc ctcgcgacgt cggccgtgat tgcccgcaac gaagcgcagt
    14221 tgcagcgcga gcgggccgag aaggaagccg agactgcgcg caagacctcc gcgttcatgg
    14281 tgggactgtt caaggtggct gacccgagcc aggcgcgcgg gcagatcacc gcggtggaac
    14341 tgttgcaggt tggggcgcga cgcatcgaca cggaactgaa ggggcagccg ggcgtgcagg
    14401 ccacgctgat ggacacgatc gggacggtgt acacgggact cggtctgtac aacgacgcgc
    14461 acacgatgct cgagcgcgcg ctggcgcagc ggcgcgcgct ggcgccggtc gatccccggg
    14521 agatcgcgcg cagccaggtg catctggcgc aggtgctcac ggagaaggca gaactcgagg
    14581 cggcggaacg tctcttcaat gaagccatcg cggtactcca gggaagcgca agcgaattcg
    14641 tgtcggctga cgagctcgcc gcggccctgg ccggactcgc cgaggtctac ttccgtgccg
    14701 gacgctatcc ggaggccgaa ccgctgctgg ccagggtcta cgagatccgc cgcgcgcggt
    14761 tgccgccggg ggatcccgcc atcgccgatg cgctcgaaga gctcggcctg aaccagttcg
    14821 accagggcaa ctacgagcgt gccgagatct acctgcgcga tgcgctcgcg atgcgccgcg
    14881 tgtcgctggg tgatgcgccg catcctgacg ttgccgagaa cctgaacaac ctggcgctct
    14941 tgctgatgga atccagccgt ttcgaggaaa gcgaacggct gttccaggag gccatgacca
    15001 tgaacagcgc gctgtacggc gcggttcacc ccgacgtggc cgctggactg aacaatctcg
    15061 gcctgctcta ccgctcgcag ggtgatctcg ccaaggccga ggaaagctac agcaacgccc
    15121 tcggcatgca gcgccagctc cggggcgaga agcatccgga agtggcgcgg gtgcagagca
    15181 acctggcttt cgtgtattac gacgagggcc agttggcgcg cgcgatcgac acgatgcgcg
    15241 aagccatgga gatgcagcgc gccaccagcg gcccgcgcca cccggatacc gccaacagca
    15301 tgtccgcgct gggacgctgg ctgagcgagg cgggcaagga tgccgaggcg gcggtgctgc
    15361 tgcgccaggc cctcgcgctg ctgaccgagt tgcacggcgc cgatcatccg gacgtagcga
    15421 tcgcggagct cgggctggcg cagacgcttg cgcgcacggg gccgctcgac gaggcgctcg
    15481 cgatggcgaa gagcgccgcg gccaaggtcg acgaggcctt cgggccggag cactggatcg
    15541 ccgcggtcgc cgggagcacc gagggcagcg tgctcgctgc catggggcgc cccgcggaag
    15601 ccgaaccact gctgaagcag ggctacgagg cgctgcgcga caacccctcc gcgcggccgg
    15661 cctacctgcg gcgcgcgcgc gaaagcctgg tggcgttcta cgaggagtcg ggacgcaagg
    15721 acctggccct gacgtatcgc caggaagcct ccgctgtcgc gccgggctcc tgatcgcgag
    15781 caccgcctcc gggcccgacg cccgcgtcgg aaccactcaa tcggcaatca gctcataggt
    15841 cgtggcgaag atgtcgcgac ccacgatcgc aagatcgtcc gcaccgtact cgacgagcca
    15901 gtctcccggc ttgccggtca gttcgccgcg cccgcccgga agcagcacgc gcgccgtgtc
    15961 cgtcagctgc cttgcgagca cctcgatgcc gcgcttgcgg tagcgcccgt cgtgcatcgc
    16021 cggcgtgggt gcaacgggtt catagcgttg ccggaacacc tcgcggcgca ccggccagcg
    16081 atccccggcc cgtgcctcga caatcgcatc gcccgcgtcg tagcgcacgt cgccttccag
    16141 cgtgcggcag attcccggcg tgcgcgcaaa gcgcacctgc acctcctccg ggagcttgcg
    16201 cgcccggcgc gcgccgggct ggcctgcaag cgtggctgca gtgttcatgc cggcagcgtg
    16261 gtctcgcggt gcttcagcag gtcctgccac tggccgttca cctcggacat ggccgcggtg
    16321 ccctcgcagg ccagttgccg caaccgcagc caggcaatcc agcgcgagtc gctgcgctgc
    16381 tcgagccgct cgatgccgcg caccacatgc cgcaggcgaa tgccggcttc ctcggacatc
    16441 gtggcaacac gctccatctc gttgctggac accaggccgt ggatacccga ggccagcgcg
    16501 ggcaaggccg cggtgaacag caggagccaa tcggcgtgca ggaagaagtg agccaccacg
    16561 gcgcagcccg tcagcaggaa cgcgatgccg gtggtgtggt gcaggcgatg cgacatgcgg
    16621 tggtgcgttt cggcgttacg gcggtggtaa tcggcctggc cgttcagcac gtcgagcagg
    16681 tattgcttgg actcgctgaa gcgttcgtgc gcgacgaagg cgctcccgtc cggcatgcgc
    16741 ggcaggcccg atgcaaccgc gctgcgacgc agtgcccact gctcggcgcc atccagcacc
    16801 cattgcgcgc gcccggaggc ctgcgcatcc gagcggcgcg gcacctgcgt gcaggattcc
    16861 ggtgccgcca cgaagggata gcccaggcga aggcagcgca gttcctcggc gaccaggcga
    16921 tgcgacagcc attcgccgtg ccagcgccgc cgcgaggcga gcatgaccag cacgcagatg
    16981 aacatgatca tgccgagctc gacccaggtc cagaagctgc tgtgatcgga aacgccgagc
    17041 ttgatgttgc cggccacggc ggcgaatacc gccagcgtcg cgaaggcgta gaggaacagt
    17101 gtcacgtcgc gacggatgcc ggcggccagg ttggcctggt agtccgacca gttgaagcga
    17161 tcctcgattc cggccggctc gaccggcagc gccgaggcgg ccgcgccgtc ggcgccgtgc
    17221 tcggccgcga cgccgaacca cggcctggtc gcgtcgcgcg cgaaaacctt gcggatgccc
    17281 gtgagcaggc tggcgggtgc tagcgaaccg cccaacgtca ccttcagcaa ttcgctgacg
    17341 atacccgcgc gacgttcgct gaaccgcgag ccggcgcgct gcgtgaagta gcgccgcacc
    17401 cggtcacaca gggcggggct gacgcgcccg gccgcctgcg gggcgagggg gttggcgatg
    17461 agcgcgagtt ccacgtccag cgcctgctcg tccagcgtga cgaacagcgg ggcgagcaac
    17521 tcggcaatgg aatcatcggc gccgagcagg aacaaggtcg gctcgtcgat gcgcgagatg
    17581 tcgagcagcc gcgcggtgcc cgcgaggtcg atccagatca ccggcttgct gcacagcacc
    17641 gcctcgcgca gcaggcgcac ggtgccgccc gcggggccac gagggtcgtg cccgtcccag
    17701 accaccatga gcaggtcgct gaatgccagc gccgtctcgt cgcgcaggtt gatggtgacc
    17761 gaagaggcgc ccgcgtcgtt ggcctcgccg agcaccaggt cgcgccgatc cgggccctcg
    17821 gcctgcaagc cggattgttg cgcttcggcc gcggtcatca cgcggtgcag ggggcagccg
    17881 cgacgcgccg cgatgcgcgc tgccatctcg tcggttcccg tgtacccgcc gcctatgatc
    17941 cgcgtgacgc agcgacgcgg ggcctgcgct ccgtagagct gctcgcaatc ggcgagctcg
    18001 gcgaactgtg ccgcgatgtg tgcgacgagc cggtcgatgg cggattcgat ggcgtgcaaa
    18061 gcggcctcgt cgctgggcag ccggtgtggg cggtggcccg cgacgaggat gctgaaatgg
    18121 ggatcgattg gtggcgcctg ctgcattgcc cggttccctg cgcttcggat gcagcgtaac
    18181 aaacaatcgt cgggaaccac tacacacgcc cgcgtgtccc ggcgtgctcc ggcacgcagt
    18241 gcccggcttc ccgctcgggc cggccgcctc gtggctcaga gcaccgccgc gagcaccagc
    18301 ggcaggacca gcacggccgc gagattgccc agcagcacga tcgaggccac gcgctcgggt
    18361 tcctggcggt agcgttccgc gaacaggaag ttcagcacgg ccggcggcag cgcgccgaag
    18421 acgatcagca tcgccgtgtc gcgcgcgccc aggccgagca gttgcgccgc gcaccacgcg
    18481 atcgccacgc cgagcgccgg gcgcaggaag gcccccacga gcggcgcgcc gatatcggtg
    18541 aagcggatgg tcttcatgcg cacgccgagc gcgaacagca gcagcgggat cgcgatgtcg
    18601 ccgagcatct gcagcgcgct cagcagcggc ccccagacct gcagcccgag caggctcacc
    18661 gcgagcccgg cgaaggtggc gcctaccgcc gggatgcgcc acagcgtcag cagtcgtgcg
    18721 ttggcatcga gcatgcgcgc cccgagcgag aagtgcagca ggttctcgac catgaacagg
    18781 atcaccgccg ccggcagtgc ggtctcgccc caggccagca ccgccagcgg tatgccgatg
    18841 ttgccggagt tgttgaacat catc
//
LOCUS       DAORUQ010000041        18849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2563_length_18849_cov_6.071353, whole genome shotgun sequence.
ACCESSION   DAORUQ010000041 DAORUQ010000000
VERSION     DAORUQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_18849_cov_6.071353"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLW13_05600"
     CDS             <1..607
                     /locus_tag="PLW13_05600"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060156.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQY69880.1"
                     /translation="ILARDETVRADSSVEALAKLAPSFQFMGDMGFDGVPRSKYPQVE
                     RIEHVHTAGNSSGIVDGAALVLIGSEAAGRKLGLKPRGRVIASGLVGTDPTIMLVGPA
                     PATRKALGVAGLRLEDIDLFEVNEAFAAVVMHFQRDTGVPWEKINVNGGAIAMGHPLG
                     ATGAMLVGTVLDELEARGQRYGVVTACVGGGMGIATVVERL"
     gene            639..2774
                     /locus_tag="PLW13_05605"
     CDS             639..2774
                     /locus_tag="PLW13_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY69881.1"
                     /translation="MKSIRYEKDADNIVHLVFDNPTESANLMNADFREDFGAAVAKLK
                     AEAELAGVILRSAKSTFFAGADLKEMFAAREQGAEAMWAVAQPLKEQMRALETLGKPV
                     VAAIGGAALGGGWELCLCAHHRIALDNPKITLGLPEVTLGLLPGGGGIVRMVRLLGIE
                     AALPFLLEGQQFNPARGKELGLVHDLAATPEELLDRARAWIKANPAAQQPYDQKGYRI
                     PGGTPATPALGPKLMIAPAMVREKTRGVMPAPEAILGCAVESTQVDIDSAMRIETRWF
                     ISLVMGTVARNMINTFWFQLNEIKAGIGRPKDVARAKFAKVGILGAGMMGAGIAHACA
                     TRGLEVVLKDVSLAAAEKGKAYSAKILGKQLAKGKLTQDKHDATLALIRPTESAADLA
                     GCELVIEAVFEDRELKARVTREAEAELAATAIFASNTSTLPITGLAGASARAANFIGL
                     HFFSPADRMPLVEIICGKDTSPETLARSYDFVQQIGKTPVIVNDARGFFTSRVFGTFC
                     HEGIAMLREGVPAAAIENGALLAGFPVGPLAVSDEVSLTLMSKIRHQTIADLRAEGKE
                     YVPHQAESVIDWMLEQNRAGKAAGKGFYEYPEGARKFLWPELATRYGRAQAPVPLADI
                     KERLLFVQAIETVRCLDEGVLTTSRDANIGSIFGIGFPAWTGGVLQYINGYGLPAFVA
                     RARELAQAYGDRFLPPASLIERAARNVDF"
     gene            2800..3942
                     /locus_tag="PLW13_05610"
     CDS             2800..3942
                     /locus_tag="PLW13_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69882.1"
                     /translation="MIPRTLFSNEHEIFRATVGRLLDEECVPHHESWEEQGHIDRAAW
                     LKAGEQGLLCPTVPEEYGGLGTDFLYNVVVDEEIARRGLSGIGWGLHSDIVAPYIVNY
                     GSAEMKRRFLPKMISGEMIGAIAMTEPGAGSDLQGVKTTAVRDGDHYVINGSKTFITN
                     GFLSDLVIVVAKTDPAGGAKGTSLILVEAGTPGFSKGRKLKKVGMKAQDTSELFFEDV
                     RVPCANLIGEEGMGFVYLMQELPQERLTVGISAVAGAEAILAQTVAYVKERKAFGKPV
                     AAFQNTQFKLAEMDSELTAMRVFIDRCIELHLQGKLDVPTVAKSKLLSTDLQCRIIDE
                     CVQLHGGYGYMWEYPVARAWADARVQRIYAGTNEVMKVIISRALLG"
     gene            4060..4902
                     /locus_tag="PLW13_05615"
     CDS             4060..4902
                     /locus_tag="PLW13_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQY69883.1"
                     /translation="MRLDYRILNVFARPGERLSGNPLCVFEDGSGLDSAQMQALALQF
                     NLSETTFILPSRTADARVRIFTPAYEMPFAGHPTLGTAHVVRSLRRCADRVRLELAAG
                     VIPVAAERDFWELRANSATTRAARASTAELAAALGLEGQDLAGPARWVDAGSEQLIVP
                     LANEDAVRRVQLSIDALRSVTGDGPRAQVYVFAPRADGSLLARFFFSKGESLLEDPAT
                     GSATANLGGWMLANGVTLPLALTIAQGEMAGRPSALRLRIDAGRQVFVAGEVIELGRG
                     VVEL"
     assembly_gap    5069..5168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5169..5722)
                     /locus_tag="PLW13_05620"
     CDS             complement(<5169..5722)
                     /locus_tag="PLW13_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69884.1"
                     /translation="MSQRETLARGDGAAPEHAGLSGVKLDLTRAEYLTLLTAMQLADW
                     VLHAHQAEESDETQPFRALEQKVFALAGDFGCEHLVEYVEEDGRFYASTEFDETGPAM
                     EFIEEFENESFWNELLERLVERDLLRQLGEKTVEGLDPEEREIREEPYRRVYGDEFAT
                     HGVDRLEILDQRYVGDAKNKRKLS"
     gene            complement(5662..6291)
                     /locus_tag="PLW13_05625"
     CDS             complement(5662..6291)
                     /locus_tag="PLW13_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQY69885.1"
                     /translation="MLLYSYPPAPNPRRLHIFMAEKGIEIPTVQVDLAKGEQFGEPLR
                     SVNPSCTVPALVTDEGVVLAQVPAICDYLEELYPERQLLGRTPLERALVREWTHRIFV
                     EGLMAIADVFRNGNPAFAKRALPGPVELEQVPALVERGMKRIDVFLHVLDAQLAGRDH
                     VVGDAFSMADIDAIATCDFLAWIRKPVPDDCRNVKRWREAMAQRPSTRA"
     gene            complement(6432..7613)
                     /locus_tag="PLW13_05630"
     CDS             complement(6432..7613)
                     /locus_tag="PLW13_05630"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQY69886.1"
                     /translation="MSDAVIVSAARTPIGKAYRGLFNNTEAPTMGGLAIRAAVERAGV
                     DPAEIDDVIMGCAMPQGTTSLNIGRMAALAAGLPVTVSGMTMDRQCSSGLMAIATAAK
                     QILHDGMRVAVGGGLEQISLVQNEHQNVYRRIDPKLKALHEHIYMPMLHTAEVVAKRY
                     GVTREQQDEYSLQSQQRTAAAQAAGLFADEIIPVTASVEMKDKATGEISHKDVTLALD
                     EGNRADTKLEGLASLKTIVEGGCITAGNASQLSDGAAACVVMDGKLAAERGIKPMGLY
                     RGIAVAGCEPDEMGIGPVFAVPKLLKQHGLKMDDIGLWELNEAFAVQVIYCRDRLGIP
                     NDRLNVNGGAISIGHPYGMSGARMVGHALREGKRRGVKYVVITMCVGGGMGAAGLFEV
                     L"
     gene            complement(7681..8598)
                     /locus_tag="PLW13_05635"
     CDS             complement(7681..8598)
                     /locus_tag="PLW13_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY69887.1"
                     /translation="MDIRYDGKVAIVTGAGAGIGRAHALGLAARGAKVVVNDLGGARD
                     GSGRSSAAADSVAAEIRAAGGEAITNGANVADWAEVQAMVRQAMDAWGRVDILVNNAG
                     ILRDKSFSKMEIGDFRAVVDVHLMGTVHCTRAVWEIMRAQQYGRIVMTTSSSGLYGNF
                     GQGNYGAAKMAVVGLMNTLVQEGEKYGIRVNAVAPAAGTRMTEGLMDQRAFELLTPEA
                     VTAGVLLLCAEQAPNRMILAAGAGGYASVHIYETPGIYLAPEAQTPENVLAGLAAIEN
                     RDGEQEFTQGFQQSLKFVGAAAKALGIEL"
     gene            complement(8692..9156)
                     /locus_tag="PLW13_05640"
     CDS             complement(8692..9156)
                     /locus_tag="PLW13_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQY69888.1"
                     /translation="MAVTMNKDNVRSLIGSELGVSDWIRIEQARVNAFADTTEDHQFI
                     HVDPERAAATPFGGTIAHGFLTLSLLPRMMEDIGGGMQDAVMGLNYGFDKIRFLAPVK
                     VNSRVRTRAKLLDVQEKVPNQFLVKQEITVEIENESKPALIAEWLTMVMVRP"
     gene            complement(9187..10401)
                     /locus_tag="PLW13_05645"
     CDS             complement(9187..10401)
                     /locus_tag="PLW13_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69889.1"
                     /translation="MDIGISARLRPILDEVRGFIDSEILPLEHEFLAGIDTGDRWAFT
                     PRQIEILEGLKARARSRGLWNFFLTEHDGGHGLTTVEYAYIAEETGKSHLAPEVFNCS
                     APDTGNMEVLARYGTPAQQEQWLKPLLAGDIRSAYVMTEPDSASSDATNISMSAVPDG
                     DEWVLNGEKIWISGAGDPRCRILIVMVKSSPGAPRHAQHSQILVPMDTPGVEVLRPMM
                     VFATDDAPHGHMHMRFTDVRVPKANIIAGEGRGFEVAQGRLGPGRIHHCMRAIGMAEK
                     ALELMCRRGLSRTAFGKPLVKLGGNYDIIANARMDIEMARLLCLKTAWMMDTEGVQQS
                     QHWISMIKTVAPTVALKVIDEAIQMHGGLGISQDTPLAGMYMGARTLRFADGPDAVHR
                     MVVARRELARFR"
     gene            complement(10434..11564)
                     /locus_tag="PLW13_05650"
     CDS             complement(10434..11564)
                     /locus_tag="PLW13_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY69890.1"
                     /translation="MSLVYNDDERMLAEAARGFLQQNASPAVLRGLRDTRDADGFSRE
                     LWREMAALGWAGMLVPEEFGGLGFAHTGAGILCEQAGRRLAASPLFATAILGATLIRE
                     AGTQAQKQVLLPAIAAGRRLCALALDESARHDPAGIALVARANGDAFRLSGEKILVLD
                     GHVADTLVVAARSRGAPGETHGISLFLVEAGSAGVAIERTVMVDSRNAAIVRFCDARG
                     ELLGALHQGWNALERTLDIGRICLAAEMLGVAGEALARTVDYLRQRKQFGRAIGEFQA
                     LQHRAAQLFCELELARSAVLRALRAIDEPDTALAPFASLAKVKAGDTAMLAVNEAVQL
                     HGGIGMTDEFEIGFFMKRAGALRQIFGDSYYHTDRYAALNGY"
     gene            complement(11597..12781)
                     /locus_tag="PLW13_05655"
     CDS             complement(11597..12781)
                     /locus_tag="PLW13_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY69891.1"
                     /translation="MSNLDEFRSETRAWLEQNCPASMRGAAASGTESCWGGRKWVFAS
                     EEQRLWLERMAARGWTAPQWPRAHGGGGLDKAEAKILGEELARLQARAPLESFGISML
                     GPALLRYGSEEQKQRFLPPIVRGEIRWCQGYSEPNAGSDLAGLQTRAEDRGDHFLVNG
                     QKVWTSYADQADWIFCLVRTDVEAPKHAGISFLLFDMETPGVSTRPIRLISGNSPFCE
                     TFFDNVRVPKENLVGELNKGWDIAKYLLTHEREMISGLFGVGAPGQAAPAGIAVQRIG
                     LRDGRLADAGLRHDLARHSIDAEAFRLTLGRVQDEAKAGVGTGAASSLLKYYGTELNK
                     KRQELLMAIHGADGLGWEGERFAEGFVPRSWLRSKGNSIEGGTSEIQLNIIARRILGL
                     PG"
     gene            12900..13763
                     /locus_tag="PLW13_05660"
     CDS             12900..13763
                     /locus_tag="PLW13_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HQY69892.1"
                     /translation="MSNDLQHLLDNLHVEQIDTNLFRGITPPGPNPRVYGGQALAQAM
                     NAATRSVDQARRVHSQHAYFLRPGDPARPIIYEVDPIRDGRGFSTRRVVARQGGRAIF
                     NTAISYQLPEKGFAHHEPMPAVPAPESLESDFAFYTRKATEQPGRYHAFTSHPVDYRT
                     LERLDRYDPPARAARFGVWMRANGTMPDDPGVHAQMLAYMSDSYLMSTALLPHAIAHD
                     NPALQSASLDHALWFYADFRADEWLYYHLASPLAGGGRGLNIGHVYTRDGRLVAAAAQ
                     EGLIRRVAAPG"
     gene            13871..15433
                     /locus_tag="PLW13_05665"
     CDS             13871..15433
                     /locus_tag="PLW13_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HQY69893.1"
                     /translation="MNGAEALLRTLLDAGVDVCFTNPGTSEMHFVAALDRVPGMRCVL
                     GLFEGVVTGAADGYARIARKPAATLLHLGPGLGNGLANLHNARRGDVAMVNIVGDHAT
                     YHLQYDTPLTSDVTAVAKPMSHWVHSAQSSEQLPADAARAVAAALQAPGQVATLILPA
                     DVSWEECTGGPAAMPAVPAARRVREDVIGQCAAALACGEPALILLGGHVSEQDLELAG
                     RIAAACGARVGVETFPTRLPWGAGRAACEKIPYIPEFALAHLETVRRLVLVGATEPAS
                     FFAYPNLPGRLAHEECELLTLAEPHHDRIDALQRLAEAMDAPALEPARNPLALPALPA
                     AGALSTAAVGDIIAALLPEQAIVVDEGITGGFEVFERTRTARAHEWIMQTGGSIGWGL
                     PAATGAAIAAPGRKVVCLEGDGSAMYTIQALWTMAREQLDVTVVLFANRDYAILQLEY
                     QRVGASGMGAKARSMLHIGNPDIDFVALAQSLGVAATRARDTVEFTSQFAEAMAARGP
                     RLIETMMPALGL"
     gene            15447..16826
                     /locus_tag="PLW13_05670"
     CDS             15447..16826
                     /locus_tag="PLW13_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY69894.1"
                     /translation="MQQLICALQEVLGPGGVLTGDEARSRSGSWGVMHCDAPAVLRPR
                     STAEVAAVLRLCHAAGQAVVTEGGKTGLVSGARASSGEIALSLERMHAIEQIDARGRT
                     MTVQAGATLQAVHEAAEAEGLIMPLDLGARGTATIGGNIATNAGGNQVIRYGMMREQV
                     LGLEVVLADGTVMSSLNQVLKNNTGYDLKQLFIGSEGTLGVITRAVLRLRPLMPARNS
                     ALVATDSLEHLVALLTFIERELGGRMSAFEAMWNNFYRIASRGPHLGQPPIADSYPFY
                     VLIESAGADLEAEAAHFLDALARATDLGLVQDAVLAQSEKERAAFWAIRDNIESLAVL
                     WPLAIFDISLPLATMEDYLERTGARLEQRLGELRWVTFGHLGDSNLHLAIALGGDDPA
                     LRHAVEEIVYAGLAGIGGSVSAEHGIGLEKREFLHCSRSATEIELMKTLKRALDPRNI
                     LNPERIFHL"
     gene            complement(16798..17010)
                     /locus_tag="PLW13_05675"
     CDS             complement(16798..17010)
                     /locus_tag="PLW13_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69895.1"
                     /translation="MRKIKLTSDMVQRIEDALDERKVQRRARAQAGATGERRCRPDRR
                     AAATDDKDAQAAAPAEPLKGGKSAPG"
     gene            complement(17208..17870)
                     /locus_tag="PLW13_05680"
     CDS             complement(17208..17870)
                     /locus_tag="PLW13_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HQY69896.1"
                     /translation="MKRIFALAVLLLSMPFALAAQEAAPKYVEGTHYVRLAQPVRTAD
                     PSRIEVTEVFWYGCIHCFHLEPLVNDWKKAQKPDVDFQRSPAMWNQTMAVHAQAFYAA
                     QALGVLEKLHGPLFAALNEERKKLDNEDELAAFFAAHGVKEADFRKMFNAFAVESSVK
                     QADARARSYGITGTPELVVNGTYRISGRTAGSPAEMLKVADFLVERERAARAAAKPAA
                     KS"
     gene            complement(18010..18615)
                     /locus_tag="PLW13_05685"
     CDS             complement(18010..18615)
                     /locus_tag="PLW13_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY69897.1"
                     /translation="MIKRIAVVVAACALAGNVLAAGNAEGGKALSVACGACHGADGNS
                     LAPTFPKLAGQHESYLLKQLKDIKSGARSVPTMAGQLDAMSEQDLADIAAYYAAQAAT
                     PGVAKEELVELGESVFRAGIREKGVAACAACHSPTGAGNGPARFPALSGQHADYIAAQ
                     LRAFRNDERTNDGDTRIMRDVASRLSDKELDAVSSYASGLH"
     gene            complement(18658..>18849)
                     /locus_tag="PLW13_05690"
     CDS             complement(18658..>18849)
                     /locus_tag="PLW13_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HQY69898.1"
                     /translation="VAGAPKHGDAAAWKLRFDAKGGLDGLLASVKNGLNAMPPKAMCM
                     DCTDGEYKALIEHMMKPAG"
BASE COUNT         2882 a         6507 c         6348 g         3012 t
ORIGIN      
        1 gatcctcgcg cgcgacgaga ccgtgcgcgc cgacagctcc gtcgaggcgc tggccaagct
       61 ggcgccctcg ttccagttca tgggcgatat gggcttcgac ggggtgccgc gcagcaagta
      121 cccgcaggtc gagcgcatcg agcacgtgca taccgccggc aactcctcgg gcatcgtcga
      181 cggcgcggcg ctggtgctga tcggcagcga ggctgcgggc aggaagctcg gcttgaagcc
      241 gcgcgggcgc gtgatcgcct ccggcctggt gggtaccgac cccaccatca tgctggtcgg
      301 tcccgctccc gccacgcgca aggcgctcgg cgttgccggc ctgcgcctgg aagacatcga
      361 cctgttcgag gtgaacgagg ccttcgccgc ggtggtgatg cacttccagc gcgacaccgg
      421 cgtgccatgg gagaagatca acgtcaacgg cggcgcgatc gcgatgggtc atccgctggg
      481 cgccaccggc gcgatgctgg tgggcaccgt gctcgacgag ctcgaggcac gcgggcagcg
      541 ctacggcgtg gtcaccgcct gtgtcggcgg cggcatgggc attgccaccg tcgtcgaacg
      601 cctctgaaca catcgcagcc gcgcaaggaa taccgcacat gaaaagcatc cgttacgaaa
      661 aggacgccga caacatcgtc cacctggtct tcgacaatcc gaccgaatcg gcgaacctga
      721 tgaacgccga ttttcgcgag gacttcggcg ccgccgtcgc gaaactgaaa gccgaagccg
      781 aactggccgg cgtgatcctg cgctcggcga agtccacctt cttcgccggc gccgacctga
      841 aggaaatgtt cgccgcgcgc gagcagggcg ccgaggccat gtgggccgtg gcacagccgc
      901 tgaaggagca gatgcgcgcg ctggagacgc tgggcaagcc cgtggtcgcc gcgatcggcg
      961 gtgcggcgct cggcggtggc tgggaactgt gcctgtgcgc gcaccaccgc atcgcgctcg
     1021 acaacccgaa gatcaccctc ggcctgcccg aggtgacgct gggcctgctg ccgggcggcg
     1081 gcggcatcgt gcgcatggtg cgcctgctcg gcatcgaggc tgcgctgccc ttcctgctcg
     1141 agggccagca gttcaacccg gccaggggca aggaactcgg cctggtgcac gatctcgccg
     1201 cgacgccgga ggaactgctc gacagggcgc gtgcctggat caaggcgaac cccgcggcgc
     1261 agcagccgta cgaccagaaa ggctaccgga ttcccggcgg cacaccggca acgcccgcgt
     1321 tgggccccaa gctgatgatc gccccggcca tggtgcgcga gaagaccagg ggcgtgatgc
     1381 ccgcgcccga ggcgatcctc ggctgtgcgg tggaaagcac gcaggtcgac atcgacagcg
     1441 cgatgcgcat cgagacacgc tggttcatct cgctggtcat gggcaccgtc gcgcgcaaca
     1501 tgatcaacac cttctggttc cagctcaacg agatcaaggc gggcattggt cgcccgaagg
     1561 acgtcgcgcg cgcgaagttc gcgaaggtgg gcatcctcgg tgccggcatg atgggggcgg
     1621 gcatcgcgca cgcctgcgcc acgcgcgggc tcgaagtggt gctgaaggac gtgagtctcg
     1681 cggcggccga aaaggggaag gcctattcag cgaagatcct cggcaaacag ctcgccaagg
     1741 gcaagctgac gcaggacaag cacgacgcca cgctggcgct gatccggccg accgaaagcg
     1801 ccgccgacct cgccggctgc gagctggtca tcgaggccgt gttcgaggac cgcgagctga
     1861 aggcgcgcgt gacgcgggag gccgaagccg agctcgcggc gacggcgatc ttcgcctcga
     1921 acacctcgac gctgccgatc acgggccttg ccggcgcgag cgcgcgtgcg gcgaacttca
     1981 tcggcctgca tttcttctcg ccggccgacc ggatgccgct ggtggagatc atctgcggca
     2041 aggacaccag ccccgagacg ctggcgcgca gctacgactt cgtgcagcag atcggcaaga
     2101 cgccggtgat cgtgaacgac gcgcgcggct ttttcacctc gcgcgtcttc ggcaccttct
     2161 gccacgaggg catcgcgatg ctgcgcgagg gcgtgcccgc ggcggcgatc gagaacggcg
     2221 cgctgctggc cggcttcccg gtcggtccgc tcgcggtatc cgacgaggtc agcctcacgc
     2281 tgatgtcgaa gatccgccac cagaccatcg cggacctgcg cgccgagggc aaggaatacg
     2341 tgccgcacca ggccgagagc gtcatcgact ggatgctgga gcagaaccgc gccggcaagg
     2401 cagccggcaa gggcttctac gagtacccgg agggggccag gaaattcctc tggccggagc
     2461 tggccacgcg ctacggccgc gcgcaggcgc cggtgccgct ggccgacatc aaggagcgac
     2521 tgctgttcgt gcaggcgatc gaaacggtgc gctgcctcga cgagggcgtg ctcacgactt
     2581 cacgcgacgc gaacatcggc tcgatcttcg gcatcggctt cccggcctgg accggcggcg
     2641 tgctgcagta catcaacggc tacggcctgc ccgccttcgt ggcgcgcgcg cgcgagctcg
     2701 cgcaagccta cggcgaccgc ttcctgccgc cggcttcgct gatcgagcgc gccgcacgca
     2761 acgtagactt ctgaaccgcg acccgaggag aagcgcacca tgattccacg cacgctgttc
     2821 agcaacgaac acgagatctt ccgcgccacg gtcggccgcc tgctcgacga ggagtgcgtc
     2881 ccgcaccacg agagctggga ggagcaaggt cacatcgacc gcgccgcctg gctgaaggcc
     2941 ggcgagcagg ggctgctctg ccccacggtg cccgaggagt acggcggcct cggcacggac
     3001 ttcctctaca acgtggtggt cgacgaggag atcgcccggc gcgggctctc gggcatcggt
     3061 tggggcctgc acagcgacat cgtggcgccg tacatcgtca actacggcag cgcggagatg
     3121 aagcgcaggt tcctgccgaa gatgatttcc ggcgagatga tcggcgcgat cgcgatgacc
     3181 gagccgggtg cgggttccga cctgcagggc gtgaagacca ccgcggtgcg cgacggcgac
     3241 cactacgtta tcaacggctc caagactttc atcaccaacg gcttcctgtc ggacctggtg
     3301 atcgtggtcg cgaaaaccga tcccgccggc ggcgccaagg gcaccagcct gatcctggtc
     3361 gaggccggca cgccgggctt cagcaagggc cgcaagctga agaaggtggg catgaaggcg
     3421 caggacacct cggagctgtt cttcgaggac gtgcgcgtgc cctgcgccaa cctgatcggc
     3481 gaggaaggca tgggcttcgt ctacctgatg caggagctgc cgcaggagcg cctcacggtg
     3541 ggcatttccg ccgtcgccgg cgccgaggcg atactggccc agaccgtggc ctacgtgaag
     3601 gagcgcaagg ccttcggcaa gccggtggcc gcgttccaga acacgcagtt caagctggcc
     3661 gagatggatt cggagctcac cgcgatgcgg gttttcatcg accgctgcat cgagctgcac
     3721 ctgcagggca agctcgacgt gcccacggtc gccaagagca agctgctgtc cactgacctg
     3781 cagtgcagga tcatcgacga gtgcgtgcag ttgcacggcg gctacggcta catgtgggag
     3841 tacccggtgg cgcgcgcgtg ggccgatgcg cgcgtgcagc gcatctacgc cggcaccaac
     3901 gaagtgatga aggtgatcat ttcgcgcgcg ctgctgggat gagttccggc gtgggtccgg
     3961 cttcagccgg acaatcgacc ggataaatcc ggacccacgc cggggcaatt gctacgatat
     4021 gcctgctgtc cggctgaagc cggacccacg gggcgaatca tgcggctgga ctaccggatc
     4081 ctgaacgtct tcgcgcgccc cggcgagcgc ctgagcggca acccgctgtg cgtgttcgag
     4141 gacggcagcg ggctcgacag cgcgcagatg caggcgctgg ccctgcagtt caacctctcc
     4201 gagaccacct tcatcctgcc ctcgcgcacg gccgatgcgc gcgtgcgcat cttcaccccg
     4261 gcttatgaaa tgcccttcgc cggacacccg acactgggca ccgcgcacgt ggtgcgctcg
     4321 ctcaggcggt gcgccgaccg ggtgaggctc gaactggcgg cgggagtgat accggtcgca
     4381 gccgagcgcg acttctggga gctgcgcgcc aacagcgcca ccacgcgcgc agcgcgcgcc
     4441 tcgaccgcag aactggcggc cgcgctcggc ctggagggac aggatctcgc gggtccggcg
     4501 cgatgggtcg atgcgggcag cgaacagctg atcgtgccac tcgccaacga agacgcggtg
     4561 cgccgcgtgc agctgtccat cgatgccctg cgaagcgtca cgggcgacgg cccgcgtgcg
     4621 caggtctacg tgttcgcgcc ccgcgccgac ggcagcctgc tggcgcgctt cttcttcagc
     4681 aagggtgaat cgctgctcga ggacccggcc accggcagcg caaccgcgaa cctcggcggc
     4741 tggatgctgg ccaatggtgt caccttgccg ctggcgctga cgatcgccca gggcgaaatg
     4801 gcaggacggc cctcggcgct gcgcctgcgc atcgacgccg ggcgccaggt gttcgtcgcg
     4861 ggcgaagtga tcgaactcgg gcgcggcgtc gtggagctgt gagcatgggc cgatggctga
     4921 tcgttgccgg gctcgtgctg gtcgcgatcg gcgcggcact gcactacgcg ccgtggctgc
     4981 tgaactggtt cggccgcctg cccggggaca tcaatatcca gggtggccgc acgcgcgttt
     5041 tcatcccgat cacctcgatg atcgtggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnta cgagagcttg cgcttgttct tcgcgtcgcc cacgtagcgc tggtcgagaa
     5221 tctcgagacg gtcgacgcca tgcgtggcga actcgtcgcc atacacgcgc ctatagggtt
     5281 cctccctgat ctcgcgctcc tcggggtcca gaccctcgac ggtcttctcg ccgagctggc
     5341 gcagcaggtc gcgctcgacg aggcgttcca gcagttcgtt ccagaacgat tcgttctcga
     5401 actcctcgat gaactccatg gcgggaccgg tctcgtcgaa ctcggtgctg gcgtaaaaac
     5461 gcccgtcttc ctcgacgtat tcgaccaggt gttcgcaacc gaagtccccg gcaagggcga
     5521 agaccttctg ctccagtgcg cggaagggct gcgtttcgtc ggattcctcg gcctggtgcg
     5581 cgtgcagcac ccagtcggcc agctgcatcg cggtgagcag cgtgaggtat tcggcgcgcg
     5641 tgagatcgag cttcacgccg ctcaggcccg cgtgctcggg cgctgcgcca tcgcctcgcg
     5701 ccagcgtttc acgttgcgac aatcgtccgg gacaggcttg cggatccagg caaggaaatc
     5761 gcaggtcgcg atggcatcga tatcggccat cgagaacgcg tcgccgacga catgatcgcg
     5821 cccggccagc tgggcatcga gcacgtgcag gaagacgtcg atgcgcttca tgccgcgctc
     5881 gaccagcgcc gggacctgct cgagctccac cgggccgggc aacgcgcgct tggcgaaggc
     5941 gggattgccg ttgcggaata cgtcggcgat ggccatcagg ccctcgacaa agatgcggtg
     6001 ggtccactcg cgcaccagcg cccgttcgag cggcgtgcgc ccgagcaact ggcgctcggg
     6061 atagagctcc tcgaggtaat cgcagatcgc ggggacctgc gccagcacca cgccctcgtc
     6121 ggtgaccagc gccggtaccg tgcacgacgg gttgaccgag cgcaacggtt ccccgaactg
     6181 ctcgcccttg gccaggtcga cttgcaccgt cggaatctcg atgcccttct cggccatgaa
     6241 gatgtgcagg cgccggggat tgggcgcggg aggataggaa taaagcagca tggcaatgct
     6301 cctcggaatg tgcggcgcag tgtacccgtg atgccgcagc cgtgcgaggc gcaagctgcc
     6361 gtgggcgatc aatgcgtggg tccggcgtca gccggacatt cggttgttcg gctgacgccg
     6421 gacccacggg atcagagcac ctcgaacagg cccgccgcac ccatgccgcc gcccacgcac
     6481 atcgtgatca cgacgtactt cacgccgcgg cgcttgccct cgcgcagggc gtggccgacc
     6541 atccgcgcgc cactcatgcc ataggggtga ccgatcgaga tcgcgccgcc attgacgttc
     6601 agtcgatcgt tcgggatgcc gaggcggtcg cggcagtaga tcacctgcac cgcgaaggcc
     6661 tcgttcagtt cccacaaacc gatatcgtcc atcttcaggc cgtgctgctt gagcagcttg
     6721 ggcaccgcga acaccggtcc gatgcccatt tcgtcgggct cgcagccggc gacggcgatg
     6781 ccgcgataca ggcccatcgg cttgatgccg cgctcggcgg cgagcttgcc gtccatcacc
     6841 acgcaggccg ccgcgccatc ggagagctgg ctggcgttgc ccgccgtgat gcagccgccc
     6901 tcgacgatcg tcttcaggct ggcgagcccc tcgagcttcg tgtcggcgcg gttgccttcg
     6961 tcgagcgcga gcgtcacgtc cttgtggctg atctcgccgg tggccttgtc cttcatctcc
     7021 accgaggccg tcaccgggat gatctcgtcg gcgaacaggc cggccgcctg cgcggcggcg
     7081 gtgcgctgct ggctctgcag cgagtactca tcctgctgct cgcgcgtgac gccgtagcgc
     7141 ttggccacca cttcggcggt atgcagcatc ggcatgtaga tgtgctcgtg cagcgccttc
     7201 agtttcgggt ctatgcggcg gtacacgttc tggtgctcgt tctgcaccag gctgatctgc
     7261 tccagtccgc cgcccaccgc gacgcgcatg ccgtcgtgca ggatctgctt ggcggccgtg
     7321 gcgatcgcca tcaggcccga ggagcactgg cggtccatgg tcatgccgct caccgtgacc
     7381 ggcaggccgg cggccagggc ggccatgcgc ccgatgttca ggctggtggt gccctgcggc
     7441 atcgcgcaac ccatgatcac gtcgtcgatc tcggccgggt ccacgcccgc gcgctcgacg
     7501 gcggcgcgga tcgcgagccc ccccatcgtg ggcgcctcgg tgttgttgaa caggccgcga
     7561 taggccttgc cgatcggcgt gcgtgccgcg gatacgatga ctgcgtctga cattttcctg
     7621 actctcctgc gcgttgtaag gatctgccgc cgcaccagcg cgagggagcg gcggcgggca
     7681 tcaaagctcg atgcccagcg ccttggcggc tgcgcccacg aacttcaaac tctgctggaa
     7741 accctgcgtg aactcctgtt cgccgtcgcg attctcgatg gcggcgagcc ctgccagcac
     7801 attttccggt gtctgcgcct ccggcgccag atagatgccg ggggtttcgt aaatatggac
     7861 gctcgcgtaa ccgccggcac cggccgccag gatcatgcgg ttgggcgcct gctcggcgca
     7921 gagcagcagc acgccggcgg tcacggcctc gggcgtcagc agttcgaagg cgcgctgatc
     7981 catcagcccc tcggtcatgc gcgtgcccgc tgccggggcg accgcgttga cgcggatgcc
     8041 gtatttctcg ccttcctgca ccagcgtgtt catgagcccg accacggcca ttttcgccgc
     8101 gccatagttg ccctgcccga aattgccgta gagcccgctg gaggaggtgg tcatcacgat
     8161 gcgcccgtac tgctgcgcgc gcatgatctc ccatacggcc cgggtgcagt gaaccgtgcc
     8221 catcaggtgc acgtcgacga cggcgcggaa gtccccgatc tccatcttcg agaagctctt
     8281 gtcgcgcagt atgccggcgt tgttcaccag gatgtcgacg cggccccagg catccatggc
     8341 ctggcgcacc atcgcctgca cctcggccca gtcggccacg ttggcgccgt tggtgatcgc
     8401 ctcgccgccg gcggcgcgga tttccgctgc cacgctatcg gccgcggcgc tggagcgccc
     8461 gctgccgtcg cgcgccccgc cgagatcgtt gaccaccacc ttcgcgccgc gcgccgcgag
     8521 acccagcgcg tgcgcgcgcc cgatcccggc gcccgcgccg gtcactatcg cgaccttgcc
     8581 gtcgtaacgg atgtccatgt ctctctcctg gcgtcttgtc agttatcgcg ctggcgggct
     8641 cgcacacatc ttagccgtat gcaccaactg cctgcccaca ggcatgcggg cctacgggcg
     8701 taccatcacc atcgtgagcc attcggcgat cagcgccggc ttgctctcgt tctcgatctc
     8761 gaccgtgatc tcctgcttga ccaggaactg gttcggcacc ttttcctgca cgtcgagcag
     8821 tttcgcgcgc gtccgtaccc ggctgttcac cttcaccggc gcgaggaagc ggatcttgtc
     8881 gaagccgtag ttgaggccca tcaccgcgtc ctgcatgccg ccgccgatgt cctccatcat
     8941 gcgcggcagc agcgacagcg tgagaaagcc gtgcgcgatg gtgccgccga agggcgtggc
     9001 cgccgcgcgc tcggggtcga cgtggatgaa ctggtggtcc tcggtggtgt cggcgaaggc
     9061 gttgacgcgc gcctgctcga tgcggatcca gtcggagacg ccgagttcgc tgccgatcaa
     9121 cgaacgcacg ttgtccttgt tcatcgtcac tgccatgtcg aaactcctct gtcgtgtcga
     9181 attcgatcag cgaaagcgcg cgagctcgcg ccgcgccacc accatgcggt gcaccgcgtc
     9241 gggcccgtcg gcgaagcgca gcgtgcgcgc gcccatgtac atgcccgcga gcggcgtgtc
     9301 ctgcgagatg cccagcccgc cgtgcatctg gatcgcctcg tcgatgacct tcagcgccac
     9361 cgtgggagcg acggtcttga tcatcgagat ccagtgctgc gactgctgca cgccctcggt
     9421 gtccatcatc cacgcggtct tcaggcacag caaccgcgcc atctcgatgt ccatgcgcgc
     9481 gttggcgatg atgtcgtagt tgccgccgag cttcaccagc ggcttgccga aggccgtgcg
     9541 cgagagcccg cgccggcaca tcagttccag cgccttctcg gccatgccga tcgcgcgcat
     9601 gcagtggtgg atgcgccccg gcccgaggcg gccctgcgcg acctcgaagc cgcgcccctc
     9661 gccggcgatg atgttggcct tcggcacgcg cacgtcggtg aaacgcatgt gcatgtggcc
     9721 gtggggcgca tcgtccgtgg cgaaaaccat catcgggcgc agtacctcga cgcccggcgt
     9781 gtccatgggc accaggatct gcgagtgctg cgcgtggcgc ggcgccccgg ggctgctctt
     9841 caccatcacg atgaggatcc ggcaacgcgg atcgccggcg cccgatatcc agatcttctc
     9901 gccgttcagc acccattcgt cgccatcggg caccgcgctc atgctgatgt tggtcgcgtc
     9961 cgacgaggcg ctgtcgggtt cggtcatcac gtaggccgag cggatatcgc ccgccagcag
    10021 cggcttgagc cactgctcct gttgcgccgg cgtgccgtag cgcgcgagca cctccatgtt
    10081 gccggtgtcc ggcgcgctgc agttgaacac ctccggcgcc aggtgcgact tgccggtttc
    10141 ctcggcgatg taggcgtact ccacggtcgt gagcccgtgg cccccgtcgt gctccgtgag
    10201 gaagaagttc cacagcccgc gcgagcgcgc cctggccttc aatccctcga ggatctcgat
    10261 ctggcgcggc gtgaaggccc agcgatcgcc cgtgtcgatt ccggccagga actcgtgctc
    10321 cagcggcagg atctcgctgt cgatgaagcc cctgacctcg tcgaggatgg gtcgcaagcg
    10381 cgcgctgatg ccgatgtcca tgcccgtgcc gctcctggtg tgccgtgccg cgctcagtag
    10441 ccgttgagcg cggcgtagcg atcggtgtgg tagtagctgt cgccgaagat ctggcgcaat
    10501 gcgccggcgc gcttcatgaa gaagccgatc tcgaattcgt cggtcatgcc gatgcccccg
    10561 tgcagctgca cggcctcgtt gactgccagc atcgcggtgt cgccggcctt gaccttcgcc
    10621 aggctggcga aaggtgccag cgcggtgtcg ggctcgtcga tcgcgcgcag cgcgcgcaac
    10681 accgccgatc gcgccagctc cagctcgcag aacagctgcg ccgcgcggtg ctgcagcgcc
    10741 tggaactcgc cgatggcgcg cccgaactgc ttgcgctgcc tgaggtaatc aacggtgcgc
    10801 gcgagcgcct cgcccgccac acccagcatt tccgccgcca ggcagatgcg gccgatgtcg
    10861 agtgtgcgct ccagcgcgtt ccagccctgg tgcagcgcgc cgagcagctc gccgcgcgcg
    10921 tcgcagaagc gcacgatcgc cgcattgcgg ctgtcgacca tcacggtgcg ctcgatggca
    10981 acccccgcgc tgccggcctc gaccaggaac aggctgatcc cgtgggtctc gccgggcgcg
    11041 ccacggctgc gcgcggccac gacgagcgtg tcggcgacgt ggccatcgag caccaggatc
    11101 ttctcgcccg agagccggaa agcatcgccg ttggcacgcg cgaccaacgc gatacccgcc
    11161 gggtcgtggc gcgcggactc atcgagcgcg agcgcgcaca gccgtcgccc cgcggcgatc
    11221 gccggcagca gtacctgttt ctgtgcctgc gtcccggcct cgcggatcag cgtggcgccg
    11281 aggatcgcag tcgcgaacag cggcgaggcc gcgagcctgc gcccggcctg ctcgcagagt
    11341 atgccggcac cggtgtgggc gaagccgagt ccgccgaatt cttccggcac cagcatgccc
    11401 gcccagccca gcgcggccat ctcgcgccag agctcgcgcg aaaagccgtc ggcgtcgcgc
    11461 gtatcgcgca gcccgcgcag caccgcgggc gaggcattct gctgcaggaa accgcgcgcg
    11521 gcctcggcga gcatccgttc gtcgtcgttg tagaccaggg acatgttcgc tccttcgcgc
    11581 gccgatccgg ccgtgctcag ccgggcaggc cgaggatgcg cctggcgatg atgttgagct
    11641 ggatctcgga ggtcccgccc tcgatcgagt tgcccttgct gcgcagccag ctgcgcggca
    11701 cgaagccctc ggcgaagcgc tcgccctccc agccgagccc gtcggcgccg tggatggcca
    11761 tcaacagttc ctggcgcttc ttgttgagct cggtgccgta atacttcagc agcgaggatg
    11821 ccgcgccggt gccgacgccg gccttcgcct cgtcctgcac gcgcccgagc gtgagccgga
    11881 atgcctcggc atcgatcgag tggcgcgcca gatcgtgacg cagacccgca tcggcgaggc
    11941 gtccgtcgcg caggccgatg cgctgcaccg cgatcccggc cggcgcggcc tgcccggggg
    12001 cgccgacgcc gaacagtccg ctgatcatct cgcgctcgtg cgtcagcagg tacttggcga
    12061 tgtcccagcc cttgttcagc tcgccgacca ggttttcctt cggcacgcgc acgttgtcga
    12121 agaaggtctc gcagaacggc gagttgccgc tgatcagccg gatcggccgg gtgctgacgc
    12181 cgggcgtctc catgtcgaac agcaggaaac tgatgccggc gtgcttcggc gcctcgacgt
    12241 cggtgcgcac caggcagaag atccagtcgg cctggtcggc gtaggaggtc cagaccttct
    12301 gcccgttgac gaggaagtga tcgccccgat cctcggcccg ggtctgcagg ccggcaagat
    12361 cggagcccgc gttcggttcg ctgtagccct ggcaccagcg gatctcgccg cgcacgatcg
    12421 gcggcaggaa gcgctgcttc tgctcctcgc tgccatagcg cagcagcgcc ggccccagca
    12481 tcgagatccc gaagctctcg agcggcgcgc gtgcctgcag gcgcgcgagt tcctcgccca
    12541 ggatcttcgc ctcggccttg tcgagaccac ccccgccatg ggctcgcggc cattgcggcg
    12601 cggtccagcc gcgcgcggcc atgcgctcca gccacaggcg ctgctcctcg ctggcgaaca
    12661 cccacttgcg cccgccccag cagctctcgg tgccggaggc cgctgcaccg cgcatcgagg
    12721 ccgggcagtt ctgctccagc caggcacggg tttcgctcct gaattcgtcg aggttgctca
    12781 tgtttgccgc ccctgttgtc cttgcggtgg cgcgaggcgg caacgttatc accaatggcg
    12841 ccggtggtac catgccgccc cgagacacag caattatccg gatcgccctg ggagtgacgg
    12901 tgagcaacga cctgcagcat ctgctcgaca acctgcacgt ggagcagatc gacaccaacc
    12961 tgtttcgcgg catcacgccg cccggaccca acccgcgcgt ctacggcggc caggcgctcg
    13021 cgcaggccat gaacgccgcg acgcgcagcg tcgaccaggc gcgccgcgtg cactcgcagc
    13081 acgcgtactt cctgcgcccg ggcgaccccg cgcggccgat catctacgag gtcgacccga
    13141 tccgcgacgg gcgcggcttc agcacgcgtc gcgtggtggc gcgccagggc gggcgcgcca
    13201 tcttcaacac cgcgatctcc taccagctgc ccgagaaagg attcgcccac cacgagccaa
    13261 tgcccgcggt gccggcgccc gagtcactgg agagcgactt cgccttctac acgcgcaagg
    13321 cgaccgaaca gcccgggcgc tatcacgcct tcaccagcca cccggtcgat taccgcacgc
    13381 tcgaacgcct cgaccgctac gatccgccgg cgcgcgcggc gcgcttcggc gtgtggatgc
    13441 gcgccaacgg cacgatgccc gacgatcccg gcgtgcacgc gcagatgctc gcgtacatgt
    13501 ccgacagcta cctgatgtcg acggcgctgc tgccgcacgc catcgcccac gacaatcccg
    13561 cgctccagag cgcgagcctc gatcacgcgc tgtggttcta cgcggacttt cgcgccgacg
    13621 aatggctgta ctaccacctg gcgagcccgc tcgcgggcgg cggccgcggc ctgaacatcg
    13681 gccacgtcta cacccgcgac gggcgcctcg tcgccgcggc cgcgcaggaa gggctgatcc
    13741 gccgggtcgc cgcgccgggc tgaaagtccg ggagtttttc accgccctgc cggggctgca
    13801 ccggccctgc cgacgcacga aggattgcgc tatcctgccc gcgcagcccc cagccccgga
    13861 gaacaacgac atgaacggag ccgaagccct gttgcgcacg ctgctggatg ccggcgtgga
    13921 cgtctgcttc acgaatcccg gcacctcgga aatgcacttc gtggcggcgc tggaccgcgt
    13981 gcccggcatg cggtgcgtgc tgggcctgtt cgaaggcgtg gtcaccggcg cggcggacgg
    14041 atacgcgcgc atcgcgcgca agcccgccgc cacgctgctg cacctggggc cggggctcgg
    14101 caacgggctg gcgaacctgc acaacgccag gcgcggcgac gtggcgatgg tgaacatcgt
    14161 aggcgatcac gcgacctatc acctgcagta cgacaccccg ctcacctcgg atgtgaccgc
    14221 ggtcgcgaag cccatgtcgc actgggtgca ctcggcgcag tccagcgagc agctgccggc
    14281 ggatgccgca cgcgcggtcg ccgccgcgct gcaggcgccc gggcaggtcg cgacgctgat
    14341 actgcccgcg gacgtgtcgt gggaggaatg cacgggcggg cccgccgcga tgcccgcagt
    14401 cccggcggcg cggcgcgtgc gcgaggacgt gatcggacag tgcgccgcgg cgctggcctg
    14461 cggcgagccg gcgctgatac tgctcggcgg gcatgtcagc gagcaggatc tggagctcgc
    14521 cgggcgaatc gccgcggcct gcggcgcgcg cgtcggggtg gagaccttcc ccacgcggct
    14581 gccctggggc gcgggccgcg ccgcctgcga gaagattccc tacattcccg agttcgcgct
    14641 cgcgcacctc gagaccgtgc gccgcctggt gctggtcggc gccacggagc ctgcctcgtt
    14701 cttcgcctac ccgaacctgc ccggccggct ggcgcacgag gaatgcgagc tgctcacgct
    14761 ggccgagccg caccacgacc gcatcgacgc gctgcagcgc ctggccgagg ccatggatgc
    14821 gccggcgctc gaaccggcgc gcaacccgct cgcattgccc gcgctccccg ctgccggcgc
    14881 gctgtccacg gctgccgtgg gcgacatcat cgcggcgttg ctgccggagc aggccatcgt
    14941 cgtcgacgag ggcatcaccg gcggcttcga ggtgttcgag cgcacgcgca ccgcacgcgc
    15001 gcacgagtgg atcatgcaga ccggcggctc catcggctgg ggcctgccgg cggcgaccgg
    15061 agcggcgatc gccgctcccg ggcgcaaggt cgtttgcctg gaaggcgacg gcagcgcgat
    15121 gtacacgatc caggcgctgt ggaccatggc ccgcgagcaa ctcgacgtca ccgtggtgct
    15181 gttcgccaac cgcgattacg cgatcctgca gctcgagtac cagcgcgtcg gcgccagcgg
    15241 catgggcgcc aaggcgcgca gcatgctgca catcggcaac cccgacatcg atttcgtggc
    15301 gctggcgcag tcactcggcg tggccgcgac acgcgcgcgc gacaccgtgg aattcacctc
    15361 acagttcgcc gaggcgatgg cagcgcgcgg accgcgcctc atagaaacca tgatgcccgc
    15421 gctcgggctc tgagggaggc accgccatgc agcaattgat ctgcgccctg caggaggtgc
    15481 tgggacccgg tggggtgctg accggggacg aggcgcgttc gcgcagcggc tcgtggggcg
    15541 tgatgcattg cgacgcgccg gcggtgctgc gcccgcgatc gaccgcggag gtggcagcgg
    15601 tactgaggct ctgtcacgcc gcgggccagg cggtggtcac cgaaggcggc aaaacggggc
    15661 tggtgagcgg ggcgcgcgcc tcatcgggcg agatcgcgct atcgctggag cgcatgcacg
    15721 cgatcgagca gatcgatgcg cgcgggcgca cgatgaccgt gcaggccggg gccacgctgc
    15781 aggcggtgca cgaggcggcc gaggccgagg gcctgatcat gccgctcgac ctgggcgcac
    15841 gcggcacggc aaccattggc ggcaacatcg ccaccaacgc gggcggcaac caggtgatcc
    15901 gctacggcat gatgcgcgag caggtgctcg ggctcgaggt cgtgctggcc gacggtaccg
    15961 tgatgtcgtc gctgaaccag gtgctgaaga acaacaccgg ctacgacctc aagcagctgt
    16021 tcatcggcag tgaaggcacg ctcggcgtga tcacacgcgc cgtgctgcgc ttgcgccccc
    16081 tgatgccggc gcgcaattcc gcgctggtcg ccaccgactc gctggaacac ctggtcgcgc
    16141 tgctgacgtt catcgagcgc gagctcggag ggcgcatgag tgccttcgag gccatgtgga
    16201 acaatttcta ccgcatcgcc agccgcggac cccacctcgg ccagccgccg atcgccgaca
    16261 gctacccgtt ctacgtgctg atcgagtccg cgggtgcgga cctggaggcc gaggccgcgc
    16321 atttcctcga cgcgctggcg cgcgccaccg atctcggact ggtgcaggac gccgtgctgg
    16381 cgcagagcga aaaggaacgc gcggctttct gggcgatccg cgacaacatc gagagcctgg
    16441 ccgtgctgtg gccgttggcg atcttcgata tcagcctgcc gctggcgacg atggaggatt
    16501 acctcgaacg caccggcgcg cggctggaac aacggcttgg cgaactgcgc tgggtcacct
    16561 tcgggcatct gggcgacagc aacctgcacc tggccatcgc gctgggcggc gacgatccgg
    16621 ccctgcgcca cgcggtggag gaaatcgtct acgccgggct ggcgggaatc ggcggctcgg
    16681 tgtccgcgga gcacggcatc gggctggaga agcgcgagtt cctgcactgt tcgcgctcgg
    16741 ccacggagat cgagctgatg aaaacgctca agcgcgcgct ggacccgcgc aacatcctca
    16801 acccggagcg gattttccac ctttgagcgg ttcggcaggt gccgccgcct gggcatcctt
    16861 gtcatcggtc gcggcagccc ggcgatccgg gcggcagcgc cgctccccgg tggctccggc
    16921 ctgcgcgcgc gcgcggcgct gcaccttgcg ctcgtcgagg gcgtcctcga tacgctggac
    16981 catgtcgctg gtcagtttga tctttcgcat cgtcattgct tgcccgccac ggaacgtcgg
    17041 gtcccgcgta tccggcaccg tgtcccgcgc attggcgtga cggtcacacg gctagtttct
    17101 gggtagcaca agcatagacc ggggtgggcg atttatcgaa tcacgccgga tgtcggcggg
    17161 gcggaacgcg cccgcggccg ggcgaccgcg ggcggcatcg ggacctgtca gctcttggcg
    17221 gcgggcttgg cggcagcgcg cgcggcacgt tcgcgctcga ccaggaaatc ggccactttc
    17281 agcatctcgg ccggcgaacc cgccgtcctg ccggagatgc ggtaggtacc gttgacgacc
    17341 agctcgggcg tgccggtgat gccgtagctg cgcgcgcgcg catcggcctg cttaacgctg
    17401 ctctcgacgg caaaggcatt gaacatcttg cggaaatcgg cttccttcac gccgtgggcg
    17461 gcgaagaagg cggccagctc gtcctcgttg tcgagcttct tgcgctcctc gttgagcgcg
    17521 gcgaacagcg gcccgtgcag cttctcgagc acgccgagcg cctgcgcggc atagaaggcc
    17581 tgcgcatgca ccgccatggt ctggttccac atcgccggcg agcgctggaa gtcgacgtcg
    17641 ggcttctgcg ccttcttcca gtcgttgacc agcggctcga ggtggaagca atggatgcag
    17701 ccgtaccaga acacttccgt cacctcgatc cgcgagggat cggcggtgcg cacgggctgg
    17761 gccaggcgca cgtagtgggt gccctcgacg tatttcggtg cagcctcctg cgccgccagc
    17821 gcgaagggca tcgacagcaa cagcacggcc aaggcaaaga tccgcttcat tcgctcagtc
    17881 tcctgtccag atgggattgt tcacccggta ccgagatcgg accgggcgtg acgggattgg
    17941 tttccgggcg atcctggaag cacgctcgaa caggtatttt tacctgttcg agcgcggcac
    18001 gtgccggtgt cagtgcaggc cggaagcgta gctcgatacc gcgtcgagct ccttgtcact
    18061 gagtcgcgac gcgacgtccc gcatgatgcg ggtgtcgccg tcgttggtgc gctcgtcatt
    18121 gcggaacgcg cgcaactgcg cagcgatgta gtccgcatgc tggccgctca gcgcggggaa
    18181 tctcgcgggg ccgttgccgg ccccggtcgg ggaatggcag gccgcgcagg ccgccacgcc
    18241 cttctcgcgg atgccggcac ggaagacgct ctcgcccagc tcgaccagtt cctccttggc
    18301 cacgcccggc gtcgcggcct gcgccgcgta atatgcggcg atatccgcga gatcctgctc
    18361 gctcatggcg tcgagctgac cggccatcgt gggaacgctg cgcgcgccgc tcttgatgtc
    18421 cttcagctgc ttcagcaaat agctctcatg ctgcccggca agcttcggga acgtcggcgc
    18481 cagactgttg ccgtcggcgc catggcaggc gccgcaggca accgacaggg ccttgccgcc
    18541 ctccgcgttg cccgcggcaa gcacattgcc cgccagcgcg cacgcggcaa ctaccacagc
    18601 gattctcttg atcacgatat cggtccttta tggtcgatgg gagcgcggca accggcctca
    18661 gccggcgggc ttcatcatgt gctcgatgag cgccttgtac tctccgtcgg tgcagtccat
    18721 gcacatggcc ttcggcggca tcgcgttcag gccgttcttc acgctcgcga gcaggccatc
    18781 cagcccgccc ttggcgtcga aacgcagctt ccacgcggcc gcatcgccgt gcttcggcgc
    18841 gccggcaac
//
LOCUS       DAORUQ010000042        18791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2576_length_18791_cov_5.983721, whole genome shotgun sequence.
ACCESSION   DAORUQ010000042 DAORUQ010000000
VERSION     DAORUQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18791
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_18791_cov_5.983721"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..614)
                     /locus_tag="PLW13_05695"
     CDS             complement(366..614)
                     /locus_tag="PLW13_05695"
                     /inference="COORDINATES: protein motif:HMM:NF022644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 domain-containing protein"
                     /protein_id="HQY69899.1"
                     /translation="MNGTSGAFELEVDLAIPAAELLRLYRGEVREVVARARDGRVLRF
                     PVAALRPFVSRDGVHGRFRIRCDAGQRLAGIERVSCPL"
     gene            complement(611..5461)
                     /locus_tag="PLW13_05700"
     CDS             complement(611..5461)
                     /locus_tag="PLW13_05700"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HQY69900.1"
                     /translation="MNDSRSRERFLGFLGEEIERRAPGDEQAVLKAFAAEVIGAMDFE
                     DVRDRRPADVCATILHAWQYLQQHDPAAPKISLFNPRFEQHGWSSRHTAVLVLTHGIP
                     FVSESLRLELNRRGIVIHMLVSSDLTVQRDEAHALRHVYPATVPPDGERRTREALVYL
                     EISRISDPALLAEIEQSLAAVIAEVTLVVDDFEPMCARVRELVAALPACSQGVAEAEW
                     QENRALLEWLLDGNFTFLGYELLEVERGDGATRVTSRGEAKLGLQRERDTPAAGFLEQ
                     EIGELERDPAAQGPQLVFFKSSRRSRVHRVAYPDYIAVNCYDAGGRISAQHCFLGLFT
                     AVVYTMDPAEIPIVRRKVAEVIERSRPSTSSHRLRTLRRVLEVLPRDELFQGDTATLY
                     DTAMRIFLIQERRKIRLFIRTDRRNRFAFCLVYWPRDIYRTELRQKIESLLRDALGAE
                     ESEFTTFFSESVLVRTYFVMRLGANARTDYDAEELEALIVRISQQWQDQLGTALAEEF
                     GEEEGARRLALYAQAFPAGYRDDNDTPMAVADIRSFDALERDGGLGVHLYRNVRESEK
                     LTHFRLYSPDRPVELSDVIPMLENLGMRVLGERPYRLLRADRREFWVHDFNLAYALAG
                     DIDVPAVAPAVEDAFLSVLAGRAESDGFNRLIVGTRLGWREVAILRAYAQYMKQVRYN
                     FSQEFIAETLGRHLGIARALVQLFRARFDPAASADMDARREAEQALAAGILADLERVE
                     QLNEDRVLRAYLALIRGTLRTNWFQCDEAGAPREWFSFKFDSAALPNLPRPVPRYEIW
                     VYSPRVAGVHLRGGKVARGGLRWSDRPEDFRTEVLGLVKAQQVKNAVIVPVGAKGGFV
                     PRQLRPDAGREAVQAEGVACYRIFVQGLLDLTDNIVDGATVAPAQVVCHDEPDPYLVV
                     AADKGTASFSDVANELSARYGFWLRDAFASGGSVGYDHKKMAITARGAWISVQRHFRE
                     RGIDVQAQPVSVVGIGDMSGDVFGNGMLRSRAIRLVAAFNHQHIFVDPDPDPAASFAE
                     RERLFALPRSSWADYDASLISAGGGVFARSAKSIALTPGMRRVLGTEAERLTPAELVS
                     VILAAPVDLVFNGGIGTYFKSSGEGHADVGDKANDAVRIDGARIRARVIAEGGNLGMT
                     QLARVEFASGGGACNTDFIDNSGGVDCSDHEVNIKILLNGIVATGDMTDKQRVQLLEE
                     MRDDVAALVLANNYRQAQSISLAEREAALRINEHRAVIASLEAAGVLDRALEFLPADE
                     ALEERRLGGRGLTRPELAVLSCYVKGRLKLDILACDVPEEAYFARALERAFPARLCER
                     YRERLPQHALRREIIATQLANDIVDLMGITFIERMQQSTNAPVAAIVRAFVMAREVLD
                     LHRWWGEVEALDNIVPAALQLEVLADLQRLTRLAARWFVHNRRGVTDASAEVEAFAAP
                     VRAIRDRMCDYVTGQQRLAWERRHEELLQAGLPPALATAFAGSTMLIGALGMADAART
                     HGLAVEEVARVAFALNERLDFYWFGKQITALKVEDHWQALARESFLDELDWQVRAIVA
                     VVAGGAGEGDDIDERVANWEATRAPAIERWRRMVAAIRDSRTQDYAMYAVAVRALLQL
                     AQPVVS"
     gene            5732..6682
                     /locus_tag="PLW13_05705"
     CDS             5732..6682
                     /locus_tag="PLW13_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQY69901.1"
                     /translation="MSTRSDTIAGLRAWLAQQVIGQQHLVDSLLIALLADGHLLVEGA
                     PGLAKTRAIRELARGVEGDFHRIQFTPDLLPGDVTGTDIYRPEDGGFRFQRGPIFHNL
                     VLADEINRAPAKVQSALLEAMAERQVSVGSTTYALPALFLVMATQNPIEQEGTYPLPE
                     AQLDRFLLHVRVGYPDAHAEREILRLARREAGQAPVAPPPAVSQQDVFAARAEILALH
                     MAASVEEYIIQLALATRGGEPLRRWLDYGVSPRGTIALDRAARAHAWLKGRDFVAPDD
                     VQAVVHDAFRHRLILNFDAEAAGTGPDQVIDELLTAVPVG"
     gene            6693..7643
                     /locus_tag="PLW13_05710"
     CDS             6693..7643
                     /locus_tag="PLW13_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQY69902.1"
                     /translation="MARRREATIAPATGPYCEMQDLLGLRAAATRLQLQPPSRALGEL
                     AGTLRSARRGRGIEFEEVRAYEPGDDVRTIDWRVTARTGRTHTRIFREERERPVFLLV
                     DQRQGMFFGSRRTLKSVQAVHAAALLAWATLAGGDRIGGLVLGEHGHHEVRPQRDRHA
                     ALALLRTCHDFNRRLGIPAAATAAAATLTEALTSTRRVARPGSLLFLISDFGGWDEAA
                     ARQLHLLARHSEVCAIHIHDPLELALPAHGRASVSDGARRLAIDAGDAALRERYGALF
                     RAEREALRAAFARVRAPLIPLPTDAPALEVLARYFKGGAR"
     gene            7640..8113
                     /locus_tag="PLW13_05715"
     CDS             7640..8113
                     /locus_tag="PLW13_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HQY69903.1"
                     /translation="MNAADPLAALRPLHLPPAVGWWPPAPGWWLLAALTLLALGAALA
                     LWLRARRRNRYRRRARAELEALYRATRGAGDAAGFAQGASALLRRAALARYARAEVAP
                     LCGESWLAFLDRSGRTSAFTGGAGRALVLAPYDPAASCDQDALHAACRDWLRRHR"
     gene            8110..9129
                     /locus_tag="PLW13_05720"
     CDS             8110..9129
                     /locus_tag="PLW13_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY69904.1"
                     /translation="MIEFALPWAAALLPLPLLVYLLLPAARRREPALYFPRAVQAAAG
                     AGSAAARARAPWPRLLALALIWIALVAAACAPRWVGDTVALPASARDLMLAVDISESM
                     LTEDLELDGQPADRLRVVKRVSGEFLRRRAGDRLGLILFGSEAYLHVPLTFDHATLER
                     LLMEAQIGFAGKQTAIGDALAIAVKRLRERPAASRVVILLTDGANTAGELPPRQAAEL
                     AARAGLRVYTIGIGAESMELPGLFGSRFGARRVNPSADLDEDTLRDIAGQTGGRYFRA
                     RDREELETIYAELDRLEPVAQEAERYRPLVSLQHWPLGFALLLSLALALGLLWPRRGA
                     ADGRT"
     gene            9134..10984
                     /locus_tag="PLW13_05725"
     CDS             9134..10984
                     /locus_tag="PLW13_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY69905.1"
                     /translation="MAALADFHFLRPAWLLALLPCALLAFWFLRRRARPALWSELIDP
                     ALLAALLLPATGARRNALPWLLLAGWALAVLGLAGPAWERLPEPVHRASDPLIIALDL
                     GHTMRAGDLAPSRYERARFKLGDILARRHDGQTALIVYAGDTHVVTPLSDDARTLAAM
                     LPALSPDIMPAAGNDLPAAVELAGSLARGAGVARGRLLVVTDRFPAAQHAEVARRLEA
                     SGLELSLLASGTADGAPIPLPQGGFLRERDGSIVVPGVDIDTMREGVDAAGGRFALLS
                     LDDSDIAALLPPPLATDIDRDDGLTRTFDRWQDRGAWLALALLPLAALAFRRGWLLGI
                     ALLLLLPQPRAEALEWQDLWLNKDQQGARAMRDQDPARAAQLFRSPAWRGAAQYEAGD
                     HAGAAVTWAAQDDADAHYNRGNALARAGRLEEALAAYDEALARAPGMDDAVANRKLVD
                     EAMKQRQQRQGQGGQQQDGQQGQEGQQDSQQDGQSPADGQPQSPEGSQGDNGSKPPEA
                     APPQDGQGNDPGEESGADGEPKADAPGKQAAQPDAQEQEQPGAGEGGAVAEGGAEARA
                     RGEAGNGEQDQAVEQWLRQVPDDPGALLRRKFEYEYRARREGAPGRGTEL"
     gene            10981..12768
                     /locus_tag="PLW13_05730"
     CDS             10981..12768
                     /locus_tag="PLW13_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQY69906.1"
                     /translation="MRLQARAWLLLMLLLAFAGGASAAVSAQVDRERIALSESIELTI
                     RVAGSMRAEEPDLAPLERHFEVLGSTRNSSFTMSNGRSESSTTWQVTLMARHAGELTI
                     PAIEVGGERTQPIAIRVSEDPAPASAAAREVQLQVETDATEVHVQQQLLLTVRLLHAV
                     NFERGATLEAPEIPDAVVRELGENSYEKLIDGRRYGVFERRYAVFPQRSGELVVPALG
                     FQAALGGASWFDRSGGRGRMLRLRSEEKRIRVLPPEAGATPWLPARVLTVIETWDRSP
                     QELRVGDSATRTVTITATGLTGAQLPPLPAPAVDGLRFYPDQPRIEDAQDAEGITGTR
                     SESSAVIPSRAGDIEFPEIRVRWWDTANQRFEEAVVPRRSIRVLPGASAAPAPAPAPL
                     AGAQPGASAAAPAAAASSATIAATPQETPSPATPPWPWIIATLLLALSTAFSSWQWRR
                     LALATPPSAAEPPAASADAAREAQLFAALAAACRANDASQAQALLPRWGRAAFPHAGI
                     HSASDLGGAAQDEELGAHIERLLASRYAANAAPCELGALLGRLEAARAAAVKQRKRGA
                     RTDALPPLYAAPHAVAAQANMPTMRPIDQ"
     gene            complement(12729..13670)
                     /locus_tag="PLW13_05735"
     CDS             complement(12729..13670)
                     /locus_tag="PLW13_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQY69907.1"
                     /translation="MLRLLLLAGLALLAHGVAAHEMSMAELTLRETAPGEFLLRWTAG
                     EKRTPEEELRPRWPGQCRDEAGVLRCADEGLSGTLAMDGVGRSFSAAMVKVFWLDGQS
                     RVYTLTSAQPSVQLFGSAEDGRGMGQIARAYTVLGVEHILAGIDHLLFVLALLFLVSF
                     NRRLVLTITAFTVAHSLTLALSALDLLVLRSPPVEATIALSIMLIAGEALHRQQTLSR
                     RWPALVAFLFGLVHGLGFAGALKEIGLPENHLPVALLTFNVGVELGQLLVVGLAFVAY
                     RFFARHTRFVGARRPALYAIGSIAAYWSIGRIVGMFA"
     gene            complement(13676..14545)
                     /locus_tag="PLW13_05740"
     CDS             complement(13676..14545)
                     /locus_tag="PLW13_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY69908.1"
                     /translation="MSTELPPIHSGASPIVEPPRRAAMPHWLREPLLHFVVLGSVLFA
                     IDHVIAGRADDPRTIVIDAAVDAEARQIFAAARGREPSAEEIEALRRVWLDNEVLYRE
                     GLALQVDKGDTAIRERVIFKALSVVDAGLKLPPHDESVLREWFESNRAKYDEPVRYDF
                     QEAVLSGDNSESAVHDFAAALNAGTPGDAKAGLRVFKGRPHANLVQSYGEEFAAALEA
                     MPVGEWRALPANGGLRVVRLEAVTAAKPADFERLLGVVLQDWTDATMAEQRTAAVRAM
                     AKKYTVKVEAQNG"
     gene            complement(14622..16871)
                     /locus_tag="PLW13_05745"
     CDS             complement(14622..16871)
                     /locus_tag="PLW13_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69909.1"
                     /translation="MNRGFNKRQLALAVAATISASAIAALPAAASPGKTGTYVSGDFH
                     NHSTCSDGSISMQKLIKKSTDTRDTPWGLDWFVQAGHGGTGNRNCTLAEDETLATPAY
                     PLVLSTSGTVQGPTTTWLNTNVAPAVEIKGNLTGSNDANRRMWRWQSLQEFQYPITEY
                     LASYLGKPLFLGMESVVAGHEHTSMAVISGQMPAELDEQPLPLMGGAQYTALGNADAL
                     SMWSYCFDRGDGDTSRGNRTGTSEGNNWDCSVPGSANSLDASWNATAMKLMPASGTGA
                     GERGHNKTLEALKWMVEFHPEGSYYVPAHLERAGPFNPNGNNGFNVEHLRNFNNVAPR
                     VAFGFETQPGHGASDARGEYNVLRNNFGGTTGYLDSVGGTTYGGTGIYGAQVGGVWDA
                     LLGEGRNWWFFASSDWHNRGSFGPDDRRTTQDFYPGEYQRNYTMVDSIDGTLTPQAIV
                     DGLRTGNVFTDSGQLVDRVAFVACIGNPGKHGRSNQAVESLAINAARGNTDLDKQGCA
                     TMGQKLEVPKGADVIVSIVVRDPAGKSYSPYTFANPSLAQVGIVQPLDEPVLDHVDVI
                     AGAVTGYKQPGAADYAGEWPRSWLNDVVAGTATLDSVPAAAKNTSAEVIRTFTDATWT
                     AVKRDPEFKAMTFRIPKAQASQYVRLRGTNLPASVPFETDADGNPLPDLWTNAVAVRS
                     GIPENGNLRIPCNSVGTTEFDGCPSHLGTVDGQKYVSYDVAAWADLWFYSNPIFIEVK
                     GSFEVAGVK"
     gene            17112..17735
                     /locus_tag="PLW13_05750"
     CDS             17112..17735
                     /locus_tag="PLW13_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HQY69910.1"
                     /translation="MAEIIGLFPTPFMRVPGAMGRELVAGLVAHFAAAADQANNSSPN
                     LVHTAMLRPGDSPLLVAAAALITPLLADFGALLFGERLGWSLKEMWVNVLDNGGRQSM
                     HNHANSFISGVVYLTPTHPEARTVFMKSPGGTDFAFKNDHAAVQTGPYNADKWISPPP
                     EPGDIVLFPSYLMHAVPANPGERRITMAFNAIPTHLDSWGYRVSFSG"
     gene            17779..>18791
                     /locus_tag="PLW13_05755"
     CDS             17779..>18791
                     /locus_tag="PLW13_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69911.1"
                     /translation="MNVMAPRRDARHLAALVLMVASGFAALGYQIVWTRQSALWLGHE
                     SAAVLAVVAAFFGGLALGSLALGPRIDRSTVPARWYAGCEAMIGTWSIALAFLLPAVT
                     GWLLELIGAEPSAARHWSIAFLGTFLLLLPATVPMGATMPAMERVLAGLRRGGAPIAA
                     LYAGNTLGALLGVLGAAFVLIPTFGLLVTAGVCAGMNFLCAAAAPWLVAGPADDTAAP
                     AQPQARGVLVLLAATGLLGIGYEVLVVRVLSQVAENTVYTFAILLAVYLVGTTLGAAA
                     YARGASGAGDPRRLRDRLLQALAATCALGTVTLAGAATMKAWVLQALGASMAAALAAE
                     AAIA"
BASE COUNT         2689 a         6789 c         6603 g         2710 t
ORIGIN      
        1 gcgtgccgca tcgggcaaat ccggtaatgc acgccttttc gagggattcc accaccgcga
       61 ggcgctcgag cacccatcgg tgcggcggac actctgtcgc caggacggcg acaggtagcc
      121 tacaggggtg aggcggattc cggcgaagca ctgcttcgcc ccgccgaacg cccgaaatct
      181 aggatttcgg gccgggcccc ggagggggat ttacggcgtg tccgcggcac cgatgggtgc
      241 tcgggcgccg atggctcgcg atacgccgga ttgtcgggcc gctatccaga gcacagatgg
      301 ttttgcgaca ccctcttcag ccggacatgt gcgcggaaat cgtgaatgtt cggctgaagc
      361 cgaacctaca gcggacacga caccctctcg atacccgcca gccgctgccc ggcatcgcag
      421 cggatgcgga aacgcccgtg cacgccgtcg cggctgacga agggacgcaa ggccgccacc
      481 gggaagcgca gcacgcggcc gtcacgcgca cgggcgacga cctcgcgcac ctcgccacgg
      541 tagaggcgca gcagctcggc ggcggggatc gccagatcca cctcgagctc gaacgccccg
      601 ctcgtgccgt tcacgacacc acgggctggg ccagttgcag cagcgcgcgc accgcgacgg
      661 cgtacatcgc gtagtcctgg gtgcgcgaat cgcggatcgc ggcaaccatg cgccgccagc
      721 gctcgatcgc cggggcgcgc gtcgcctccc agttcgccac gcgctcgtcg atgtcgtcgc
      781 cctcgcccgc gccgcccgcc accaccgcca cgatggcgcg cacctgccag tcgagctcgt
      841 cgaggaatga ctcgcgcgcc agcgcctgcc agtggtcctc gaccttaagc gccgtgatct
      901 gcttgccgaa ccagtagaaa tcgagccgct cgttcagcgc gaaggccacg cgcgcgacct
      961 cctccacggc cagcccgtgc gtgcgcgcgg catccgccat gcccagcgcg ccgatcagca
     1021 tcgtcgagcc ggcaaaggcc gtcgccagcg ccggcggcag gcccgcctgc agcaattcct
     1081 cgtgacgccg ctcccatgcc aggcgctgct ggcccgtcac gtagtcgcac atgcggtcgc
     1141 ggatcgcccg cacgggagcc gcgaaggcct cgacctcggc gctcgcgtcg gtcacgccgc
     1201 ggcggttgtg cacgaaccag cgcgccgcca ggcgcgtgag ccgctgcagg tccgcgagca
     1261 cctccagctg cagggccgcc gggacgatgt tgtccagcgc ctctacctcg ccccaccagc
     1321 gatgcaggtc gagcacctcg cgcgccatca cgaaggcacg cacgatggcg gccaccggcg
     1381 cgttggtgga ctgctgcatg cgttcgatga aggtgatgcc catcaggtcg acgatgtcat
     1441 tggcgagttg cgtcgcgatg atctcccggc gcagcgcgtg ctgcggcagg cgctcgcggt
     1501 agcgctcaca gagccgcgcc gggaaggcgc gctcgagcgc gcgcgcgaag tacgcctcct
     1561 cgggcacgtc gcaggcgagg atgtcgagct tgaggcgacc cttgacgtag cacgagagca
     1621 cggcgagctc ggggcgcgtg agccctcgcc ccccgaggcg gcgctcttcc agcgcctcgt
     1681 ccgcgggcag gaactccagc gcgcgatcga gcacgccggc cgcctcgagc gaggcgatca
     1741 cggcgcgatg ctcgttgatg cgcagcgccg cctcgcgctc ggcgaggctg atcgactgcg
     1801 cctggcggta gttgttcgcc agcaccagcg cggcgacgtc gtcgcgcatc tcctccagca
     1861 gctgcacgcg ctgcttgtcg gtcatgtcgc cggtggccac gatgccgttc agcaggatct
     1921 tgatgttgac ctcgtggtcc gagcagtcga cgccgccgga gttgtcgatg aagtcggtgt
     1981 tgcaggcccc gccgccactg gcgaactcca cgcgcgcgag ctgcgtcatg ccgaggttgc
     2041 cgccctcggc gatcacgcgc gcgcggattc gcgcgccgtc gatacgcacc gcgtcgttgg
     2101 ccttgtcgcc gacgtcggcg tggccctccc cgctcgactt gaagtaggtg ccgataccgc
     2161 cgttgaacac caggtccacg ggtgcggcga ggatcaccga gaccagctcg gcgggcgtca
     2221 ggcgctcggc ctccgtgccg agcacgcggc gcatcccggg cgtgagcgcg atcgatttcg
     2281 cgctgcgcgc gaacacgccg ccgcccgccg atatcaggct tgcgtcgtaa tcggcccagc
     2341 tcgagcgcgg cagcgcgaac aggcgctcgc gctcggcgaa gctcgccgcg ggatcgggat
     2401 cggggtcgac gaagatatgc tggtggttga aggcggcgac cagtctgatc gcgcgcgagc
     2461 gcagcatgcc gttgccgaac acatcgcccg acatgtcgcc gatacccacc acgctgaccg
     2521 gctgcgcctg cacgtcgatg ccgcgctcgc gaaagtgccg ctgcaccgag atccacgccc
     2581 cgcgcgccgt gatcgccatc ttcttgtggt cgtagcccac gctgccgccg gaggcgaagg
     2641 catcgcgcag ccagaagccg tagcgcgccg agagctcgtt ggcgacgtcg gagaacgagg
     2701 ccgtgccctt gtcggcggcg accaccaggt agggatccgg ctcgtcgtgg cagaccacct
     2761 gcgccggcgc gacggtggcg ccgtcgacga tgttgtcggt gaggtcgagc agcccctgca
     2821 cgaagatccg gtaacaggcc acgccctcgg cctgcaccgc ctcgcggccg gcgtcggggc
     2881 gtagttgccg cggcacgaag ccgcccttgg cgcccaccgg cacgatcacc gcgttcttca
     2941 cctgctgcgc cttcaccagc cccagcacct cggtgcgaaa gtcctccggc cggtccgacc
     3001 agcgcagccc gccgcgcgcg accttgccgc cgcgcaggtg cacgcccgcc acgcgcggcg
     3061 aatacaccca gatctcgtat ctgggcaccg ggcgcggcag gttgggcagc gcggcgctgt
     3121 cgaacttgaa cgagaaccac tccctgggcg cgcccgcctc gtcgcactgg aaccagttgg
     3181 tgcgcagcgt gccgcggatc agcgccaggt acgcgcgcag cacgcggtcc tcgttgagct
     3241 gctcgacgcg ctcgagatcc gcgagaatgc cggccgccag cgcttgctcg gcctcgcggc
     3301 gcgcgtccat gtccgcgctg gccgccggat cgaagcgcgc gcgaaagagc tgcaccagtg
     3361 cgcgcgcgat gccgaggtga cgccccagcg tctcggcgat gaactcctgc gaaaagttgt
     3421 agcgcacctg cttcatgtac tgcgcatagg cgcgcaggat cgcgacctcg cgccagccga
     3481 gacgcgtgcc cacgatcagt cggttgaagc cgtcgctctc ggcgcgcccc gcgagcacgc
     3541 tgaggaaagc gtcctccacg gccggggcca ccgcgggcac gtcgatgtcc ccggccagcg
     3601 cgtaggcgag attgaaatcg tgcacccaga actcgcgccg gtcggcgcgc aacagccggt
     3661 aggggcgttc gccgagcacc cgcatcccga ggttctcgag catcgggatc acgtcggaga
     3721 gttcgaccgg gcgatcgggg ctgtagagcc ggaagtgggt cagcttctcc gattcgcgca
     3781 cgttgcggta caggtgcacg cccagcccgc cgtcgcgctc gagcgcgtcg aagctgcgga
     3841 tgtcggccac cgccatcggc gtgtcgttgt cgtcgcggta gccggcgggg aacgcctgcg
     3901 cgtagagcgc gaggcggcgc gcgccctcct cctcgccgaa ttcctcggca agtgcggtgc
     3961 cgagctggtc ctgccattgc tgcgagatgc gcacgatcag cgcctctagc tcctcggcgt
     4021 cgtagtcggt gcgcgcgttc gcgcccaggc gcatcacgaa gtaggtgcgc accagcaccg
     4081 attcggagaa gaaggtggtg aactcggatt cctcggcccc cagcgcgtcc ctcagcagcg
     4141 actcgatctt ctgcctgagc tcggtgcggt agatgtcgcg cggccagtac accaggcaga
     4201 aggcgaaacg gttgcgccgg tcggtgcgga tgaacaggcg gatcttgcgt cgctcctgga
     4261 tcaggaagat gcgcatcgcg gtgtcgtaga gcgtggcggt atcgccctgg aacagctcgt
     4321 cgcgcggcag cacctcgagc acgcgcctga gcgtgcgcag gcggtggctg ctggtgctcg
     4381 ggcgcgagcg ctcgatcacc tcggcgacct tgcgccgcac gatcgggatc tccgcgggat
     4441 ccatcgtgta gaccaccgcg gtgaacagtc cgaggaaaca gtgctgcgcg ctgatgcgcc
     4501 ccccggcgtc gtagcagttc accgcgatgt agtccggata ggcaacgcgg tgcacgcgcg
     4561 agcggcgcga ggacttgaag aacaccagct gcgggccctg cgccgcaggg tcgcgctcca
     4621 gttccccgat ctcctgctcc aggaagcccg ccgccggcgt gtcgcgctcg cgctgcagcc
     4681 cgagcttcgc ctcgccgcgg ctggtgacgc gcgtcgcgcc gtcgccgcgc tcgacctcga
     4741 gcagctcgta gccgaggaac gtgaagttgc cgtccaggag ccactccagc agcgcgcggt
     4801 tctcctgcca ctcggcctcg gcaaccccct gcgagcacgc gggcagcgcg gcgaccagct
     4861 cgcgcacgcg tgcgcacatg ggttcgaaat cgtcgacgac cagcgtgacc tcggcgatca
     4921 ccgcggcgag gctctgttcg atctcagcca gcagcgcggg gtcgctgatg cgcgagatct
     4981 ccaggtacac cagcgcctcg cgcgtgcgcc gctcgccgtc cgggggcacc gtcgcggggt
     5041 agacgtggcg cagcgcgtgc gcctcgtcgc gctgcaccgt gagatccgag ctcaccagca
     5101 tgtggatcac gatgccgcgg cggttgagct ccaggcgcag cgactcggac acgaagggaa
     5161 taccgtgcgt cagcaccagc accgcggtgt ggcgcgagct ccagccgtgc tgctcgaagc
     5221 gcgggttgaa caggctgatc ttcggcgccg ccggatcgtg ttgctgcagg tattgccagg
     5281 cgtgcaggat ggtcgcgcag acatcggccg ggcgccggtc gcggacgtcc tcgaagtcca
     5341 tggcgccgat gacctcggcg gcgaaggcct tgagcaccgc ttgttcgtca cccggcgcgc
     5401 gccgctcgat ctcctcgccc aggaagccca gaaaacgctc cctgctcctg ctgtcgttca
     5461 tgcctgcacc cgttgttcga ataatgtcgc cgcggtggcg cgccgcgcgt cgaaggcgcc
     5521 gcggccctgg cgttgcgcgg cgaggatact atggaacctc gcaccgccgc accggtcgaa
     5581 gcggggcgaa agttgcgcgt ggcaagcgcc ttgcggacat cggcgcagac tacaatcagc
     5641 gacttgcttg tgggtccggc ttcagccgga ctgatgtccg gataaatccg gacccacggc
     5701 cggacccacg aacctcggag aacaggacaa cgtgagcaca cgcagcgaca ccatcgccgg
     5761 ccttcgcgcc tggctggcgc aacaggtcat cgggcagcag cacctggtcg acagcctgct
     5821 gatcgcgctg ctcgccgacg gccacctgct ggtcgagggt gcgccggggc tggcgaagac
     5881 gcgcgccatc cgcgaactgg cgcgcggcgt cgagggcgac ttccaccgca tccagttcac
     5941 ccccgacctg ctgccgggcg acgtcaccgg taccgatatc taccgtcccg aggacggggg
     6001 ctttcgcttc cagcgcggcc cgatcttcca caacctcgtg ctcgccgacg agatcaaccg
     6061 cgcgccggcc aaggtgcagt cggccctgct ggaggcgatg gccgagcgcc aggtcagcgt
     6121 gggcagcacc acctacgcgc tgccggcgct gttcctggtg atggcgacgc agaacccgat
     6181 cgagcaggaa ggcacctacc cgctgcccga ggcgcaactc gaccgcttcc tgctgcacgt
     6241 gcgcgtgggc tatccggatg cgcacgccga gcgcgagatc ctgcgtctcg cgcggcgcga
     6301 ggcgggccag gcgcccgtgg cgccaccgcc cgccgtgagc cagcaggacg tgttcgccgc
     6361 gcgcgccgaa atcctcgcgc tgcacatggc cgcgagcgtc gaggaataca tcatccagct
     6421 cgcgctggcc acgcgcggcg gcgagccgct caggcgctgg ctcgactacg gcgtgagccc
     6481 gcgcggcacc atcgcgctcg atcgcgccgc gcgcgcgcac gcctggctga agggccgcga
     6541 cttcgtcgcg cccgacgacg tgcaggccgt ggtgcacgat gccttccgcc accgcctgat
     6601 cctcaacttc gacgccgagg cggccggcac cggtccggac caggtcatcg acgagctgct
     6661 gaccgcggtg ccggtgggct gagcgacccg tcatggcgcg gcgcagggaa gccaccatcg
     6721 ccccggccac ggggccgtac tgcgaaatgc aggacctgct cggcctgcgc gcggcggcca
     6781 cgcggctgca gctgcagccg ccgagccgcg cgctcggcga gctcgccggc acgctgcgca
     6841 gcgcgcggcg cgggcgcggc atcgagttcg aggaggtgcg cgcctacgag ccgggcgacg
     6901 acgtgcgcac catcgactgg cgcgtcaccg cgcgcaccgg ccgtacgcac acgcgcatct
     6961 tccgcgagga gcgcgagcgc ccggtgttcc tgctggtcga ccagcgccag ggcatgttct
     7021 tcggcagccg ccgcacgctg aagtcggtgc aggcggtgca tgccgcggcg ctgctggcgt
     7081 gggcgacgct cgccggtggc gatcgcatcg gcggactggt gctgggcgag cacgggcacc
     7141 acgaggtgcg cccgcagcgc gaccgtcacg ccgcgctcgc gctgctgcgc acctgccatg
     7201 acttcaaccg ccgcctcggc atccccgccg ctgcgactgc cgcggcggcc acgctcaccg
     7261 aggcgctcac cagcacgcgc cgcgtggcgc gcccgggcag cctgctgttc ctgatcagcg
     7321 acttcggcgg ctgggacgag gccgccgcgc ggcagctgca cctgctcgcg cgccacagcg
     7381 aggtgtgcgc gatccacatc cacgacccgc tcgagctggc gctgcccgcg cacgggcgcg
     7441 cgagcgtctc ggacggcgcg cgccgcctcg ccatcgacgc cggcgacgcg gcgttgcgcg
     7501 agcgctacgg cgcgctgttc cgcgccgagc gcgaggcgct gcgggcggcg ttcgcgcgcg
     7561 tgcgcgcgcc gctgatcccg ctccccaccg acgcaccggc actggaggtg ctggcgcgct
     7621 acttcaaggg aggcgcgcga tgaacgccgc cgatccgctg gcggcgctgc gcccgctgca
     7681 cctgccgccc gccgtgggct ggtggccgcc ggcgccgggc tggtggctgc tcgcggcact
     7741 gacgctgctc gcgctcggcg cggcgctcgc gctgtggctg cgcgcgcggc ggcgcaaccg
     7801 ctatcgccgc cgcgcgcgcg ccgagctcga ggcgctgtac cgcgccacgc gcggcgcggg
     7861 cgatgcggca ggcttcgcgc aaggcgcgag cgcactgttg cgccgtgccg cactcgcccg
     7921 ctatgcgcgc gccgaggtcg cgccgctgtg cggcgagtcg tggctcgcgt tcctggaccg
     7981 cagcgggcga acctcggcat tcaccggcgg cgccggccgc gcgctggtgc tcgcgcccta
     8041 cgaccccgcg gcgtcctgcg accaggacgc gctgcacgcg gcctgccgcg actggctgag
     8101 gcggcaccgg tgatcgagtt cgcgctgccc tgggccgcgg cgctgctgcc gctgccgctg
     8161 ctggtgtacc tgctgctgcc ggcggcgcgc cggcgcgaac cggcgctgta tttcccgcgc
     8221 gcggtgcagg ccgctgccgg cgccggcagc gcggcggcgc gcgcgcgtgc gccgtggccg
     8281 cgcctgctcg cgctggccct gatctggatc gcgctggtgg cggcggcctg cgcgccgcgc
     8341 tgggtgggcg acaccgtcgc gctgccggcg agcgcgcgcg acctgatgct ggcggtggac
     8401 atctccgaga gcatgctcac cgaagacctc gagctcgacg gccagccggc cgaccgcctg
     8461 cgcgtggtga agcgcgtctc gggcgaattc ctgcgccggc gcgccggcga ccggctgggg
     8521 ctaatcctgt tcggcagcga ggcctacctg cacgtgccgc tcaccttcga tcacgccacg
     8581 ctggagcggc tgctgatgga ggcgcagatc ggcttcgccg gcaagcagac cgcgatcggc
     8641 gatgcgctcg cgatcgcggt gaagcgcctg cgcgaacggc cggcggccag ccgcgtggtg
     8701 atcctgctca ccgatggcgc caacaccgcg ggcgagctgc cgccgcgcca ggccgcggag
     8761 ctggcggcac gcgcggggct gcgcgtctac accatcggca tcggcgccga gagcatggaa
     8821 ctgccgggac tgttcggcag ccgcttcggg gcgcgccgcg tgaacccctc ggcggacctc
     8881 gacgaggaca ccctgcgcga catcgccggg cagaccggcg ggcgctattt ccgcgcccgc
     8941 gaccgcgagg agctcgagac gatctacgcc gagctcgacc ggctggagcc ggtggcgcag
     9001 gaggccgagc gttaccgccc gctggtctcg ttgcagcact ggccgctcgg cttcgcgctg
     9061 ctgctctcgc tcgcgctggc gctcggcctg ctgtggccgc gccgcggcgc cgccgatgga
     9121 aggacctgag gcgatggccg cactcgcgga tttccatttc ctgcgccccg cgtggctgct
     9181 cgcgctgctg ccctgcgcgc tgctcgcgtt ctggttcctg cgtcggcgcg cgcggccggc
     9241 actgtggagc gaactgatcg accccgcgct gctcgcggca ctgctgctgc ccgcgaccgg
     9301 cgcccggcgc aacgccctgc cctggctgct gctcgcgggc tgggcgctcg cggtgctggg
     9361 gctcgcgggg ccggcctggg agcgcctgcc ggagcccgtg catcgcgcca gcgatccgct
     9421 gatcatcgcg ctcgacctcg gccacaccat gcgcgccggc gatctggcgc cctcgcgcta
     9481 cgagcgcgcg cgcttcaagc tcggcgacat cctcgcgcgg cgccacgacg ggcagaccgc
     9541 gctgatcgtc tacgcgggcg acacgcacgt ggtcacgccg ctgtccgatg acgcgcgcac
     9601 gctggcggcg atgctgccgg cgctctcgcc cgacatcatg ccggcggccg gcaacgacct
     9661 gcccgcggcc gtcgagctgg ccggatcgct ggcgcgcggg gccggcgtgg cgcgcgggcg
     9721 gctgctggtc gtgaccgatc gcttcccggc ggcgcagcac gccgaagtgg cccggcgtct
     9781 cgaggcatcg ggcctcgagc tgtcgctgct cgcgagcggc accgcggacg gcgccccgat
     9841 cccgctgccg cagggcggct tcctgcgcga gcgcgacggc agcatcgtgg tgccgggcgt
     9901 ggacatcgac accatgcgcg agggcgtgga cgccgcgggc gggcgcttcg cgctgctgag
     9961 cctggacgac agcgatatcg ccgcgctgct gccgccgccg ctcgccaccg acatcgatcg
    10021 cgacgacggc ctcacgcgca ccttcgaccg ctggcaggac cgcggggcct ggctggcgct
    10081 cgcgctgctg ccgctggccg cgctcgcctt ccgccgcggc tggctgctcg gcatcgcgct
    10141 cctcctgctg ctgccgcaac cgcgcgccga ggcgctggaa tggcaggacc tgtggctcaa
    10201 caaggaccag cagggcgcgc gcgccatgcg cgaccaggac ccggcgcgcg ccgcgcaact
    10261 gttccgctcg cccgcctggc gcggcgcggc gcagtacgag gccggcgacc acgcgggtgc
    10321 cgccgtgacc tgggccgctc aggacgacgc cgacgcgcac tacaaccgcg gcaacgcgct
    10381 ggcacgcgcg gggcgactcg aggaggcgct cgcggcctac gacgaggcgc tggcgcgcgc
    10441 acccggcatg gacgacgccg tcgccaaccg caagctggtg gacgaagcga tgaagcagcg
    10501 ccaacagcgg cagggccagg gcgggcagca acaggacggg cagcaggggc aggaggggca
    10561 gcaagactcg cagcaggacg ggcaatcccc ggcagacggc cagccgcaat cgccagaagg
    10621 tagccagggg gataacggca gcaagccacc cgaggccgcg ccaccacagg acgggcaagg
    10681 caacgatcca ggcgaggaat ccggcgccga cggcgaaccg aaggccgatg cgcccgggaa
    10741 gcaggccgcg caacccgacg cgcaggagca ggagcagccc ggtgccggcg aaggcggcgc
    10801 cgtcgcagag ggcggtgccg aggccagggc ccgcggcgaa gccggcaacg gcgaacagga
    10861 ccaggcggtg gagcaatggt tgcgccaggt cccggacgat ccgggcgcgc tgctacggcg
    10921 caagttcgaa tacgagtacc gcgcgcgccg cgagggcgcg ccagggcgag gaacggaatt
    10981 atgagactcc aggcccgcgc gtggctgctg ctgatgctgc tgctcgcctt cgccgggggc
    11041 gccagcgccg cggtgtcggc gcaggtcgat cgcgagcgca tcgcactgtc ggaaagtatc
    11101 gagctcacca tccgcgtggc gggttcgatg cgcgccgagg aacccgatct cgcaccgctg
    11161 gagcgccact tcgaggtgct gggcagcacg cgcaattcca gcttcacgat gtccaacgga
    11221 cgcagcgagt cttccaccac gtggcaggtc acgttgatgg cacgccacgc cggcgagctg
    11281 accatcccgg caatcgaggt cggcggcgag cgcacgcagc ccattgcgat ccgcgtgagc
    11341 gaggatccgg cgcccgcctc cgccgcggca cgcgaggtac agctgcaggt cgagaccgac
    11401 gccaccgaag tgcacgtgca acagcagctg ctgctcacgg tgcgcctgct gcacgccgtg
    11461 aacttcgagc gcggcgcgac gctggaggcg cccgagattc cagatgccgt ggtgcgcgag
    11521 ctcggcgaga actcctacga gaagctcatc gacggccgcc gctacggcgt gttcgagcgc
    11581 cgctacgcgg tgttcccgca acgcagcggc gagctcgtgg tgccggcgct gggcttccag
    11641 gccgcgctgg gcggcgccag ctggttcgac cgctccggcg ggcgcggcag gatgctgcgc
    11701 ctgcgctcgg aggaaaaacg catccgcgtg ctgccgcccg aggccggcgc cacaccgtgg
    11761 ctgccggcgc gcgtgctcac ggtgatcgag acctgggacc ggagcccgca ggaactgcgc
    11821 gtcggcgatt ccgccacgcg caccgtgacg atcaccgcca ccggcctgac cggcgcgcag
    11881 ctgccgccgt tgcccgcgcc cgcggtggat gggctgcgct tctatccgga ccagccgcgc
    11941 atcgaggacg cgcaggacgc cgagggcatc accggcacgc gcagcgagag cagcgccgtg
    12001 atcccctcgc gcgcgggcga catcgagttt ccggaaatca gggtgcgctg gtgggatacc
    12061 gccaaccagc gcttcgagga agccgtggtc ccgcggcgct cgattcgcgt gctgccgggc
    12121 gccagcgccg cccccgcgcc cgcgcccgcg ccgcttgccg gcgcgcaacc cggcgccagc
    12181 gctgcggctc ccgccgcagc cgcgagcagc gcaaccatcg ccgcgacacc gcaggagacg
    12241 ccctcgcccg ctaccccgcc gtggccctgg atcatcgcca cgctgctgct ggcgctgagc
    12301 accgccttca gcagctggca gtggcggcgc ttggcgctgg caacaccgcc aagcgcggca
    12361 gaaccgccgg cggcgtcagc ggacgcagcg cgcgaggcgc aactgttcgc ggcgctcgcc
    12421 gcggcctgcc gcgccaacga cgcatcgcag gcgcaggcgc tgctgccgcg ctggggacgc
    12481 gccgccttcc cgcacgccgg catccattcg gccagcgacc tgggcggcgc cgcgcaggac
    12541 gaggaactgg gcgcgcacat cgagcgcctg ctggccagcc gctacgccgc gaatgccgcg
    12601 ccctgcgagc tcggcgcgct gctcgggcgg ctggaagcgg cgcgcgctgc cgcggtcaaa
    12661 cagcgcaagc gcggcgcgcg aacggacgca ttgccgcccc tgtatgccgc gccacatgcc
    12721 gtagcagctc aggcaaacat gccgacgatg cgcccgatcg accagtaggc cgcgatgctg
    12781 ccgatcgcgt agagcgcggg ccggcgcgcg ccgacgaatc gcgtatgacg cgcgaagaac
    12841 cggtaagcga cgaacgccag gcccaccacc agcagttgtc ccagttccac gcccacgttg
    12901 aaggtgagca gcgccaccgg cagatggttc tccggcagcc cgatctcctt tagcgcgccg
    12961 gcaaagccca ggccgtgcac cagcccgaac aggaaggcca ccagcgcggg ccagcgccgc
    13021 gacagggtct gctgccggtg cagcgcctcg ccggcgatca gcatgatcga caacgcgatc
    13081 gtcgcttcca cgggcggcga gcgcagcacg agcagatcca gcgcgctgag cgccagcgtc
    13141 aggctgtgtg ccacggtgaa ggcggtgatg gtcagcacca gccgccggtt gaagctcacc
    13201 aggaacagca gcgcgagcac gaacagcagg tgatcgatac ccgcgaggat gtgctcgacg
    13261 ccgagcaccg tgtaggcccg ggcgatctgg cccatgccgc gcccgtcctc ggccgatccg
    13321 aacaactgga cgctgggctg tgccgaggtc agcgtataga cccggctctg gccgtcgagc
    13381 cagaagacct tcaccatcgc ggcggaaaag ctcctgccca cgccgtccat cgccagcgtg
    13441 ccgctcagcc cttcgtcggc gcaacgcagc acgccggcct cgtcgcggca ctgcccgggc
    13501 cagcgcggcc tgagctcctc ctcgggggtg cgtttctcgc ctgccgtcca tcgcagcagg
    13561 aactctcccg gcgcggtctc gcgcagcgtg agttcggcca tgctcatctc gtgggccgca
    13621 acgccgtgcg ccagcagcgc cagccccgcc agtagcagca aacgcaacac gcggttcatc
    13681 cgttctgcgc ctccaccttc acggtgtact tcttcgccat cgcgcgcacc gcggcggtgc
    13741 gctgctcggc catcgtggcg tcggtccagt cctgcagcac cacgcccagc aggcgctcga
    13801 aatctgcggg cttcgccgcc gtgaccgcct cgagccgcac cacgcgcagc cccccgttgg
    13861 cgggcaacgc gcgccattcg cccaccggca tcgcctccaa ggccgcggcg aattcctcgc
    13921 cgtagctctg cacgaggttg gcgtgcggcc gccccttgaa cacgcgcagc cctgccttgg
    13981 catcacccgg cgttcccgcg ttcagcgccg ccgcgaagtc gtgcacggcg ctttcggagt
    14041 tgtcgccgga aagcacggcc tcctggaaat cgtagcgcac cggctcgtcg tatttcgcgc
    14101 gattgctctc gaaccactcg cgcagcaccg actcgtcgtg cgggggcagt ttcagcccgg
    14161 catcgaccac gctcagcgcc ttgaagatca cgcgctcgcg gattgcggtg tcgcccttgt
    14221 cgacctgcaa cgccagcccc tcgcgataga gcacctcgtt gtcgagccag acccggcgca
    14281 acgcctcgat ctcctccgcg gaaggctcgc gcccgcgcgc cgccgcgaag atctggcgcg
    14341 cctcggcgtc gaccgcggca tcgataacga tggtgcgcgg atcgtccgcg cgaccggcaa
    14401 tgacatggtc gattgcgaac agcacgctgc ccagcacgac gaagtgcagc aagggctcgc
    14461 gcagccagtg cggcatggcc gcacggcgcg gcggctcgac gatcggggaa gcgccggaat
    14521 gaatgggggg taattctgtg gacatcgttg ttcatgcacc cggaaaaaag gaaacgccgc
    14581 gcgttgccgc gcggcgtgtt tgacgcacca gcggtgaagc cttacttcac gccggccacc
    14641 tcgaacgaac ccttcacttc gatgaagatc gggttgctgt agaaccagag gtcggcccac
    14701 gccgcgacgt cgtagctcac gtacttctgg ccgtcgacgg tccccaggtg cgacgggcag
    14761 ccgtcgaact cggtggtacc gacgctgttg cacggaatcc gcaggttgcc gttctcgggg
    14821 ataccgctcc tgacggcaac cgcgttcgtc cacaggtcgg gcagcgggtt gccatcggcg
    14881 tcggtctcga agggcacgct ggccggcagg ttggtgccgc gcaggcgcac gtactgcgaa
    14941 gcctgcgcct tcgggatgcg gaaggtcatc gccttgaact ccggatcacg ctttaccgcg
    15001 gtccaggtcg cgtcggtgaa ggtcctgatc acctcggccg aggtgttctt cgctgccgcc
    15061 ggcacggagt ccagcgtggc ggtgcccgcg accacatcgt tcagccagct tcgcggccac
    15121 tcaccggcat agtcggcggc gcccggctgc ttgtagcccg tcaccgcgcc ggcgatcacg
    15181 tcgacgtggt cgagcaccgg ctcgtcgagc ggctgcacga tgccgacctg ggccagcgag
    15241 gggttggcga acgtgtaggg cgaatagctc ttgcccgcgg ggtcgcgcac cacgatcgag
    15301 acgatcacgt ccgcgccctt cggcacttcg agcttctggc ccatcgtggc gcagccctgc
    15361 ttgtcgaggt cggtgttgcc gcgcgcggca ttgatcgcca gcgactcgac cgcctggttg
    15421 ctgcgtccgt gcttgccggg gttgccgatg caggcgacga aggccacgcg atcgaccagc
    15481 tggccgctgt cggtgaagac gttgccggtg cgcaggccgt cgacgatggc ctgcggcgtc
    15541 agcgtgccgt cgatgctgtc gaccatcgtg tagttgcgct ggtactcgcc cgggtagaaa
    15601 tcctgcgtgg tacgacggtc gtccggaccg aagctgccgc ggttgtgcca gtcggagctg
    15661 gcgaagaacc accagttgcg cccttcgccg agcagcgcgt cccacacgcc gccgacctgc
    15721 gcgccgtaga tgcccgtgcc gccgtaggtc gtgccgccca ccgagtcgag gtagccggtc
    15781 gtaccaccga agttgttgcg caggacgttg tactcgccgc gcgcatccga tgccccgtgg
    15841 cccggctggg tctcgaagcc gaaggccacg cgcggtgcca cgttgttgaa attgcgcagg
    15901 tgctcgacgt tgaagccgtt gttgccgttg ggattgaacg ggccggcgcg ctcgaggtgg
    15961 gccggcacgt agtaactgcc ctcgggatgg aactcgacca tccacttcag cgcctcgagg
    16021 gtcttgttgt ggccgcgctc gccggcgccg gtgccgctgg ccggcatcag tttcatggcg
    16081 gtcgcgttcc agctggcatc caggctgttc gcgctgccgg gaacggagca atcccagttg
    16141 ttgccctcgc tggtgcccgt cctgttgccg cggctggtgt cgccatcgcc acgatcgaag
    16201 cagtaggacc acatcgagag cgcgtcggcg ttgccgagcg cggtgtattg cgcgccgccc
    16261 atgagcggca acggctgctc gtcgagttcc gccggcatct ggccgctgat taccgccatg
    16321 ctggtgtgct cgtggccggc cacgaccgac tccatgccga ggaacagcgg cttgcccagg
    16381 taggaggcaa ggtattcggt gatcgggtac tggaactcct gcagcgactg ccagcgccac
    16441 atcctgcggt tcgcatcgtt gctgcccgtg agattgccct tgatttcaac ggccggcgca
    16501 acgttggtgt tcagccaggt ggtggtcggt ccctgcaccg tgccgctggt cgagaggacc
    16561 agcgggtagg ccggcgtggc cagggtctcg tcctctgcca gcgtgcagtt gcggttaccg
    16621 gtaccgccgt ggccggcctg cacgaaccag tcgagacccc acggcgtgtc ccgggtgtcg
    16681 gtcgacttct tgatcagctt ctgcatcgag atggagccat ccgagcaggt ggaatggttg
    16741 tggaaatcac ccgagacgta ggtgccggtc ttgccggggc tggcggcggc aggcagcgcg
    16801 gcgatcgcgc ttgcgctgat cgtcgcggcc acggctagag cgagttgtcg cttgttgaat
    16861 ccgcggttca tggtactgat tcctctggtt ggagcggggc gtgttgtctg tcgtccggcg
    16921 gcgatagctg accggcactg caccgtagca agcgaatgtg aacggcccgt taacgaaaaa
    16981 tgacgcgggc accggcgccg gtgccgcatc ggccaacgcg aatcgacgag gcgcattcat
    17041 caatctgtcg tattcgcctg cctagaattg cgggttggcc agcggcggcc tctcgatccg
    17101 gacaccgatt catggcagag atcatcggac tgtttcccac gcccttcatg cgggtgcccg
    17161 gcgccatggg gcgcgagctc gtcgcgggcc tcgtcgcgca tttcgccgcg gcggccgacc
    17221 aggcgaacaa ctcctcgccc aacctcgtgc acacggccat gctgcgcccc ggcgacagcc
    17281 cgctgctcgt cgctgccgcg gcgctgatca cgccgctgct cgcggacttc ggcgcgctgc
    17341 tgttcggcga gcggctggga tggtcgttga aggaaatgtg ggtgaacgtg ctggacaacg
    17401 gcgggcgcca gtcgatgcac aaccacgcga acagtttcat ctccggcgtg gtctacctca
    17461 cgccgacgca tccggaggcg cgcacggtgt tcatgaagtc cccgggcggg accgatttcg
    17521 cgttcaagaa tgaccacgcc gcggtgcaga ccggccccta caacgccgac aagtggatca
    17581 gcccgccgcc cgagcccggc gacatcgtgc tgtttcccag ctacctgatg cacgcggtgc
    17641 cggcgaaccc cggcgagcgg cgcatcacca tggcgttcaa cgccattccg acccacctgg
    17701 attcctgggg ctaccgggtc tcgttcagcg gctgacgtgg acgcgcaacc agctcccgtc
    17761 gcaggcggca tcgccaacgt gaatgtcatg gcgccccggc gcgatgcgcg ccacctcgcc
    17821 gcgcttgtgc tgatggtggc ctcgggcttt gcggcgctcg gctaccagat cgtgtggacc
    17881 cggcagagcg cgctgtggct gggccacgag tccgccgccg tgcttgccgt ggtggccgcc
    17941 ttcttcggcg gactcgcgct cggttcgctg gcgctcggcc cgcgcatcga ccgcagcacc
    18001 gtgccggcgc gatggtacgc cggctgcgag gcgatgatcg gcacgtggag catcgcgctg
    18061 gctttcctgc tgcccgcggt gacggggtgg ctgctggagc tgatcggcgc cgagccgtcc
    18121 gccgcgcggc actggtccat cgctttcctc ggcaccttcc tgctgttgct ccccgccacc
    18181 gtgcccatgg gcgccacgat gccggccatg gagcgcgtgc tcgcgggcct gcgccggggc
    18241 ggcgccccga ttgccgcgct gtatgcaggc aataccctcg gcgccctgct gggcgtgctc
    18301 ggcgcggcct tcgtgctgat accgacgttc ggcctgctgg tcaccgcggg cgtttgcgcg
    18361 ggaatgaact tcctgtgcgc cgctgccgcg ccgtggctag tcgcggggcc cgccgacgac
    18421 acagcggcgc cggcgcaacc gcaggcgcgc ggcgtgctgg tgctgctcgc ggcaacgggc
    18481 ctgctcggca tcggctacga ggtgctcgtg gtgcgcgtgc tgagccaggt cgcggaaaac
    18541 accgtgtaca ccttcgcgat cctgctggcc gtatacctcg tgggaacgac gctcggcgcc
    18601 gccgcctacg cgcgaggcgc atcgggtgcc ggcgacccgc ggcgcctgcg cgaccgcctg
    18661 ctgcaggcgc tggccgcgac gtgcgcgctc ggtacggtga cactcgcggg cgccgcgacc
    18721 atgaaggcgt gggtgctgca ggcgctcggc gccagcatgg cggccgcgct cgcggccgaa
    18781 gccgccatcg c
//
LOCUS       DAORUQ010000043        18527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2645_length_18527_cov_5.816966, whole genome shotgun sequence.
ACCESSION   DAORUQ010000043 DAORUQ010000000
VERSION     DAORUQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2645_length_18527_cov_5.816966"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLW13_05760"
     CDS             <1..238
                     /locus_tag="PLW13_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69912.1"
                     /translation="TVVVCFLFILSGFPLVLDAVNAWAPRVLVDAIASMSFLTRFNAI
                     SKGVIALQDLLFFVALAAVWLAATAVVIEMKQAD"
     gene            249..2105
                     /locus_tag="PLW13_05765"
     CDS             249..2105
                     /locus_tag="PLW13_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HQY69913.1"
                     /translation="MNRRIYPRLALALLAVGFVSFMALNNLLFGGLRIDLTENRMYTV
                     SEGTREIIDSIEEPIDLYFFFSDKATRDVAPLRSYARRVRELLEEYARHADGRIRLQV
                     IDPVAFSEAEDQAAQFGLQAVPLDAGGEQVYFGLAGSNAWGEQQLIPFFQPEREEFLE
                     YELSKMLASLVAAKKPVVGVVSGLQINGGFDMMSSQPTPPWVVMEQLQQGFEVRNLPL
                     TGDAIDEDVTTLMVVHPAGLPDSMQYAIDQFVLKGGKAIVYVDPYCESSRASGNPMLE
                     QGAPESASSLPRLFRAWGVDFAPDKVVGDAATALQISVAADQPPVAHLGFLGLGVEQI
                     SREDVVSGSLESINLGMAGALAPAAGASTHFEPLLQSSAQSALLEAMQVQMNRDPAVL
                     LKGFAPTGERYTLAARIGGPAKSAFDGPQVEGREHVGEAKDINVIVVADADLLADRFW
                     VQVQDFFGQRTAMAWADNAAFAQNALENLSGSSALIGIRSRGRYSRPFELVQRIRLGA
                     DAKYRESAELLQQRLDETEQQLAELQRNRQESNQLALSPQQEQALARFQDEKLRIRKQ
                     LREVRLKLDQDIERLGMALKVLNIVVMPLVLTLALFGWHRWRVRRRRAADRR"
     gene            2125..3297
                     /locus_tag="PLW13_05770"
     CDS             2125..3297
                     /locus_tag="PLW13_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQY69914.1"
                     /translation="MQMNQKSRLGLAAAIVAVMVLLVAVFGHRERPGTVAGVASPWLP
                     QLQSQLGELRRLRLQSSAETVTLARGEAGWVVEERAAYPADFRQLETLLEALASARLL
                     ERKTARPEYFDRLGLVDIEQPASEAVQLELWSAGEQPVVRVLLGRAAEGRNGRYVRAV
                     SEQQTWLIDTSPEPMTNPADWLERTLLDIDFSRVASVSRALGATPEFSATRTVAGDAP
                     LALAALPAGKVPRYQGVFDAAARAILTAEPEDVRKAAELDFTGAAETVIGSFDGLNLT
                     VRAIKAKDGNWVALGAAAGTPVPRAEAPASELASDEELTPETAGTDAIPGEAAAQAER
                     LNRRLDGWAFKVSDYVYGELVKGLADYLQDEQPPAVAPGTPAQPPAAGQAGESRDE"
     gene            3290..4330
                     /locus_tag="PLW13_05775"
     CDS             3290..4330
                     /locus_tag="PLW13_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQY69915.1"
                     /translation="MNDSDILGSRRAIFAWLWLLIGMVFGMVVVGGVTRLTGSGLSIV
                     EWAPIMGTLPPLNHADWLAAFAKYQQYPQYRITHPDMTLGEFQGIFFWEYVHRLLGRL
                     VGMVFLLPFLWFWARGCLTHRLRARLLVALGLGGLQGLMGWLMVKSGLVDEPRVSHFR
                     LAAHLGLAFTIMAYLFWLVLQLRGDDGRAAAKAAAPRVLIASVAALTVLQIVYGAFVA
                     GLRAGLVYNTFPKMNGRWIAEGVMGMDPAWLSLLEHDVTIQFVHRWLGTLLLVLVAVL
                     WLRVRAGRFALHQRASAAALMGLVSLQYGLGVYTLIEQVPIVPAVMHQGCASLVLLAL
                     VWVLHGFRRTTA"
     gene            complement(4341..6014)
                     /locus_tag="PLW13_05780"
     CDS             complement(4341..6014)
                     /locus_tag="PLW13_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQY69916.1"
                     /translation="MTMEVRRTQPGLFLLLRRLRLPLILLIVVYAVAVFGFTLVPGID
                     AAGQPWRMGFLHAFYFVSFLGTTIGLGEIPQPFSDAQRLWATASIYATVTAWLYGIGA
                     LLSTLQDPLFRRILHENRFAAAVRGLREPFVLLCGYDDAGKLIARELCEEGIGVVVVD
                     RVQERVDSVETDELPLSVPALQANAMHPGTLLTAGVNHPACIATLALTGDDAANLSVS
                     LNAKILAPERQVICVAHHHEHQAAMARVGAEHLINPHDTFAERLAQALIKPSLHVIYE
                     SLTTQTSTPMAEPPAFPRGRWLVCGYGRFGRTVHRHLQQVGIEVTAVDLLAPADATID
                     HVTGSAIDAATLHRARIEHADAIVVATPNDTTNLAIAMLARELNPRLFMVLRQSERRN
                     TPLFRAVDADITTLSGYIVAAEVLRIIRAPQLSYFLRLARQQDEAWVRELLERMRERI
                     GDEIAETWSIGIDAAAMPAVAAAIRRGRKVTVGDLMRAPDNREIPLSAVPLLLQRREG
                     KSLLPGDEEALAMGDRLLLCGRDAARGRLRWTVSDDRVLRYLLRARAGR"
     gene            complement(6011..6391)
                     /locus_tag="PLW13_05785"
     CDS             complement(6011..6391)
                     /locus_tag="PLW13_05785"
                     /inference="COORDINATES: protein motif:HMM:NF040078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HQY69917.1"
                     /translation="MSFDKILFAFVIVLALTLNVGFVVGDIDDIAHHDKYELALAIVV
                     SLIATVMKFGDRSLLGSTMLATSLVADVQLIAAAVVWASATLDGAAISEHDTVIVVSL
                     AIGAMVANVISVVLLMIETASLRR"
     gene            complement(6468..7664)
                     /locus_tag="PLW13_05790"
     CDS             complement(6468..7664)
                     /locus_tag="PLW13_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69918.1"
                     /translation="MHALDWTRWILRALESSRMRSLLTATGIAIGICAVTLLTSIGEG
                     IRVYLLDSFSQFGTRIVTVTPGRTETLGGAGMLATVRPLSLEDALSLQRLAHTEAVVP
                     VILGTSKIEAGRYQRDTDVYAVGPDMPAAWRFGVAQGRFLPDDGLRNSRYFAALGAKV
                     KEELFQGRNALGEYVRIGGSRFQVIGVMESKGQLLGFDLDDAIYIPTDLGQAMFNREG
                     LMEIDLVFRASTTSKQYSERIRAHLVERHGEEDFTLFTQEDMLASLDKILTMLKFAIG
                     AIGSVALVVGGVGVLTIMSTALGERIGEIGLLRALGATRRQVLLLFLGEAVLLAALGG
                     ALGLLAVLALVLLGRAALPGLPIAIEPLYLLLSLALSCGIGLLAGIVPALNAANLDPI
                     EALRSE"
     gene            complement(7669..8877)
                     /locus_tag="PLW13_05795"
     CDS             complement(7669..8877)
                     /locus_tag="PLW13_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69919.1"
                     /translation="MRPADLLRFTLLALRRQRFRSLMQLLSVAIGVASVVVLVALGEG
                     ARRYVLGEFSFIGKDTVVLFPGRKSTTGGMPPVTGVAARDITLADMLTIQRTVAGIES
                     IAPVVVGNAPVSHGARERDSTVIGTSADFFAVRKLRIAQGSMLPSLALDIGTPVAVIG
                     QKLKGELFGNRRAIGQWVRLRDYRFRVIGVLEGRGDSFGMDLSEAIFIPVASAQAVFN
                     TNGLFRVVLKAREGVAIEPLKEALLARMTELHEGEEDVTVTSPDAMLSTFDGVLRVLT
                     LGVSGIAAISLVVAGILVMNLTLMSVSQRTPEIGLLKALGATDAQVRAIFLAEAGVLA
                     AAGAIAGMLGGRAAIALIAALYPAIPFASPLWALVAASALALATALLFAWLPAGKAAR
                     LEPVLALGRR"
     gene            complement(8864..9586)
                     /locus_tag="PLW13_05800"
     CDS             complement(8864..9586)
                     /locus_tag="PLW13_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69920.1"
                     /translation="MIELRALSRTFTLGDSEVRALRDVNLRIGRGEYVSVMGPSGSGK
                     STLLNILGLLDRPDGGEYLLNGTPTAALGEEARARLRREHVGFVFQSYHLVTRLTARE
                     NIELPLMLAGRAPRERLRAVDAVLERLGIGERAGHLPNQLSGGERQRVAIGRSIVMKP
                     DLLLADEPTGNLDSRAGAEVTALLEELNRDGITLLVVTHDATLGRRAARHLSMLDGAI
                     SGDAPAGNPGSAARAQSPDAPR"
     gene            complement(9579..10715)
                     /locus_tag="PLW13_05805"
     CDS             complement(9579..10715)
                     /locus_tag="PLW13_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY69921.1"
                     /translation="MKRILVLILAAALLLGAIWYTRREEPLPVTLHTVERGVVERTVA
                     NTRAGTIKACRRSALSMPGGGRVEVLHVKEGQEVAAGELLLELWNEDRRAMTAQAESQ
                     LAAASHRREQLCVEADNAAREARRLATLQQRKLVSEELAQAADTRARSAAFACRAAGD
                     EQQTAAASLQMNRALLAETQLRAPFAGIVAEINGEVGEFVTPSPPGIPMPPAVDLIDY
                     SCLYVTAPIDEVDAGRLKAGLPARISLDAFRGEHFAGRLDRIAPYVMEIEKQARTVDV
                     DVLFTNAADRERLLVGYSADIDLVLEAHADAIRIPSEALMDNDTVYRFDPASSRLERV
                     AVQTGIRNWNFTEITAGLAPGDRVVTSLDQQGLAEGVLATPADD"
     gene            10808..11629
                     /locus_tag="PLW13_05810"
     CDS             10808..11629
                     /locus_tag="PLW13_05810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY69922.1"
                     /translation="MEIAGKTVLITGGSAGVGLAAAQRFLQFGASVAITGRDGARLAQ
                     AAAQLGAGDRVITIAWDAVEIARAGTVFAEVERRFGALHVLVNNAGCNHRGLLEELSE
                     EEFLHVVDTNLRAPLALSRTVLPFMRRAGQGAIVNVASLAGRVPLAHESIYSATKFGL
                     RAFTLAIAQELEGSGISASVVSPGPISTGFILDDIDGVPDMVFSQPMSTAEEVADLIV
                     RCARDGRAERALPMISGWLANVAYLLPPLARVLRPAMEGRGRRAKAAYRARRSGS"
     gene            11644..12624
                     /locus_tag="PLW13_05815"
     CDS             11644..12624
                     /locus_tag="PLW13_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HQY69923.1"
                     /translation="MRLVEISAFGGPRVLRETRGPLPVPQAGEVLVRVAYAGVNRPDV
                     AQRQGVYPPPPGASPVPGLEIAGQVVATGPGCARWRVGDAVCALVAGGGYAEYATAPE
                     AQCLPLPRGLSVCEAAALPETAFTVWSNVFERGALARGELLLVHGGTSGIGTLAIQLA
                     KARGARVIATAGSEDKCAACRELGADLAVNYRETDFVTAVKEFSAGRGADVILDMVGG
                     DYTQRNIRAAAEQGRIVQIAFLQGSKAELDLMPLMLKRLVLTGSTLRARSLDFKAALA
                     RAVEQNVWPLVESGRVRPRVARVFGLDEAGAAHALMESSAHIGKIVLRID"
     gene            complement(12678..14249)
                     /locus_tag="PLW13_05820"
     CDS             complement(12678..14249)
                     /locus_tag="PLW13_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQY69924.1"
                     /translation="MKSSRYRISMAIACLAVTAAAHGGALNTLRDAARQTVLNNPEVL
                     SAFHEFQAAEQQTRVAKGGYLPRVDLEAAVGAERIDEPRFDEYDFTRDRVSLLLNQMI
                     YDGFATRNRVRSFDFAARMRYFEVLDASERLSAEVARAYYDVLRHRELVELSKDNYAA
                     HRLIYEQIERRTRAGVSRGVDLEQAFGRLALSESNLLTDVTNLHDASMRYQRIVGETP
                     AESLQEPELAPNLLPGTVNDTLALAYASNPQMNAMIANVWSANSAADVQKATMLPRFD
                     VRARQDIWNDKDDINGRHEEGVIELAMTYNLYNGGSDLAERRRLRYKANQAEDMRTMT
                     CRNVRQEVSIAYNEMRATDEQLVFLDRHQTSIEKARMAYRRQFDIGQRTLLDMLDTEN
                     EYFMSRRAYVNARRLNAISYTRALAGTGALVSALELGNDLHPMERPGTDEEAPDMAAV
                     CPPEPDVAPLIDKDAIYREAIRKQGELQHLVAPESPAATPADVSVEAMRAGTVTRGAP
                     AAATPEQPAKASDGW"
     gene            14447..15124
                     /locus_tag="PLW13_05825"
     CDS             14447..15124
                     /locus_tag="PLW13_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HQY69925.1"
                     /translation="MRLNATVLARRVLGALLAVLGLIGACLANPDLDRITQLALERYG
                     VDAGESVGLWRALIEENRDLAEREKLELVNTFFNHRVDFEQDAVVWGVRDYWATPLET
                     LGRGQGDCEDFSIAKYVTLGLLGVPLERLRITYVRAETGLPGSGIVQAHMVLAYYPEP
                     GVEPLILDNLVGQLRPASRRPDLKPVFGFNSQGLWVAGKKASADPTARLSRWRDLLRR
                     MNEEGLN"
     gene            15174..17117
                     /locus_tag="PLW13_05830"
     CDS             15174..17117
                     /locus_tag="PLW13_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HQY69926.1"
                     /translation="MSLVKQLWLAIALVMLIAFGGSFLVSTLSARNYLEEQLRLKNFD
                     NATSLAATLSQTLERTPGDEVTIELQIAAQFDLGHYESIRLVDPSGKALVEFASDAGV
                     DGAPQWFARLFRIEAEPGLAQVQNGWQPYGTLSVSSQASFAYRELWNSMLRLLGWFLA
                     AAVLTGVAGTVLLRLILRPLGAVVDQAEAIGARRFITTAEPATREFRAVVRSMNALAT
                     RVRQMLQDESSRLDQLRREAHHDAVTGLLNRSHFLARVQSALAREDAGAEGVLVIARL
                     LDLHELNREQGWAVMDTLIKRFAEALRAIAPKDAEWIFGRLNGSDFAVLAPGEDDAGA
                     LAQRVQEALQMLARELDLQAVCRLPAAATPYRHGDQLSRVLARVDVALANAVAAGGDT
                     VQVAGVEQSPPDAGMRMDLVTWRQRFDSALTMGQVKLHSYPVVDAAGQLLHDECVARL
                     RLDVDSDWLNAGEFVPWLARLGDLARLDEMVLDLAIARLLNCTDDLCMNFSAQSMNDA
                     VLMHRLASRLGETPELAKRLWLEVPEYGVFQNLENFRILSALLKPLGCRIGIEHVGHQ
                     VARIGELHDLGLDYLKIDASFVHGIDQNQANQVFLRGLAMIAHSIGLSAMAEGVGTEA
                     EFRALVELGFDGATGPAVTMRAG"
     gene            complement(17123..17446)
                     /locus_tag="PLW13_05835"
     CDS             complement(17123..17446)
                     /locus_tag="PLW13_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69927.1"
                     /translation="MPYVRRDATGQLIALSSEALEGFEEALLDAAEIAEFEHRLGDAK
                     ARLQESDRDVVRVLDDLVNLLIDKNLIRFTDLPAAAQRKLIERRGLRETGSHLGLLGD
                     DTSLL"
     gene            complement(17508..18368)
                     /locus_tag="PLW13_05840"
     CDS             complement(17508..18368)
                     /locus_tag="PLW13_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HQY69928.1"
                     /translation="MVFYLTSGNNLVAVKLNYSSSLNINDYAQPATLAAAVAAAVNAD
                     PLLEGYSVVGYAIKAGLGIHTFGTTFTDLIQNTGGNAALASGTYDGSVNISPTEIASL
                     DPIELAPPAAALSMSDVLDTTVIDDGNRQAKDDKPGERGHRASGKDDATGADPDVLPD
                     DSGDSLLGGSEGADVFKWTLAEPGAHDTISNFDMNEPASGGDVLDLRDLLPPESAASL
                     DSYLHFEDSGTGGTLVKISPAGDFTGDAAHDANVAYQTIELQNVDLLSIGNDQQIIDM
                     LINHGKLITE"
BASE COUNT         2747 a         6129 c         6651 g         3000 t
ORIGIN      
        1 caccgtggtg gtgtgcttcc tgttcatcct gagcggcttt ccgctggtgc tcgacgcggt
       61 gaacgcctgg gcgccgcgcg tgctggtcga cgcgatcgcc tcgatgagct ttctcacccg
      121 gttcaacgcg atcagcaagg gcgtgatcgc gctgcaggac ctgctgttct tcgtggcgct
      181 ggccgcggtg tggctggcgg cgaccgcggt ggtgatcgaa atgaagcagg cggattgaga
      241 gcatggcgat gaaccgcagg atctacccgc gcctggcgct ggcgctgctg gcggtcggct
      301 tcgtgagttt catggcgctg aacaacctgc tgttcggcgg gctgcgcatc gacctcacgg
      361 agaaccggat gtacacggtc tcggagggaa cgcgcgagat catcgacagc atcgaggagc
      421 cgatcgacct ctatttcttc ttttccgaca aggcgacgcg cgacgtggca ccgctgcgca
      481 gttacgcgcg acgcgtgcgc gagctgctcg aggagtacgc gcggcacgcc gacgggcgga
      541 tccggctgca ggtgatcgat ccggtcgcgt tctccgaggc cgaagaccag gccgcgcagt
      601 tcggcctgca ggcggtgccg ctcgatgccg gcggcgagca ggtgtatttc gggctcgccg
      661 gcagcaatgc ctggggcgag cagcagttga tcccgttctt ccagcccgag cgcgaggagt
      721 tcctcgagta cgagctcagc aagatgctcg cgagcctggt cgcggcgaag aagccggtgg
      781 tcggcgtggt atccgggctg cagatcaacg gcggcttcga catgatgagc tcgcagccga
      841 caccgccctg ggtggtgatg gagcagttgc agcagggctt cgaggtgcgc aacctcccgc
      901 tcacgggcga tgccatcgac gaggacgtca ccacgctgat ggtggtgcat ccggcgggcc
      961 tccccgacag catgcagtac gcgatcgacc agttcgtgct caagggcggc aaggcgatcg
     1021 tctacgtcga tccgtactgc gaatccagcc gggcctccgg caacccgatg ctggagcagg
     1081 gtgcgccgga gtccgcttcc agcctgccgc ggctgttcag ggcctggggc gtggacttcg
     1141 cgcccgacaa ggtggtcggc gatgccgcca cggcgttgca gatcagcgtg gctgccgacc
     1201 agccgccggt cgcgcatctg ggcttcctcg gcctcggggt cgaacagatc agccgcgagg
     1261 acgtggtcag cggcagcctg gagtcgatca acctcggcat ggccggcgcg ctcgcgcccg
     1321 ccgcgggagc gagcacgcat ttcgagccgc tgctgcaatc gagcgcgcag tccgcgctgc
     1381 tggaggcgat gcaggtgcag atgaaccgcg atccggcggt gctgctgaag ggcttcgcgc
     1441 cgacgggcga gcgttacacc ctggccgcgc gcatcggcgg tccggcgaaa tccgccttcg
     1501 acgggccgca ggtcgaggga cgcgagcacg tcggcgaggc gaaggacatc aacgtgatcg
     1561 tggtggccga cgccgacctg ctggctgatc gcttctgggt ccaggtgcag gatttcttcg
     1621 gccagcgcac ggccatggcg tgggccgaca acgcggcctt cgcgcagaac gcgctcgaga
     1681 acctgagcgg cagctcggcg ctgatcggga tccgcagtcg cgggcgctac tcgcgtccct
     1741 tcgagctggt gcagcggatc cgcctcggcg ccgatgcgaa gtaccgcgag agcgccgagc
     1801 tgctgcaaca gcgcctggac gagaccgagc agcagctcgc cgaactgcag cgcaaccgcc
     1861 aggagagcaa ccagctcgcg ctgtcgccgc agcaggagca ggcgctggcg cgcttccagg
     1921 acgagaagtt gcgcatccgc aagcagttgc gcgaggtgcg cctgaagctc gaccaggaca
     1981 tcgagcgcct ggggatggcg ctgaaggtgc tgaatatcgt ggtgatgccg ctggtgctga
     2041 cgctggcgct gttcggctgg catcgctggc gcgtgcgtcg gcgccgcgcc gccgaccggc
     2101 gctagcggga gggagcgaat ccgcatgcag atgaaccaga agagccggct cggcctggcc
     2161 gcggcgatcg tcgccgtgat ggtgttgctg gtcgccgttt tcggccaccg cgagcgtccc
     2221 ggcacggtgg ccggcgtcgc gtcgccgtgg ctgccgcaat tgcagtcgca gctcggcgag
     2281 ctgcgccggc tgcggctgca atcgagcgcc gagaccgtca cgctggcccg cggcgaggcg
     2341 ggctgggtcg tcgaggagcg cgcggcttac ccggcggatt tccgccagct cgagacgctg
     2401 ctcgaggcgc tcgccagcgc gcggctcctg gagcgcaaga ccgccaggcc cgagtatttc
     2461 gatcgcctcg ggctggtcga catcgagcag ccggcaagcg aggcggtgca gctcgagctc
     2521 tggagcgcgg gcgagcagcc ggtcgtgcgc gtgctgctcg gcagggcggc cgaggggcgc
     2581 aacgggcgct acgtgcgcgc ggtgtcggag cagcagacct ggctgatcga cacttcgccc
     2641 gagcccatga ccaaccccgc ggactggctc gagcgcacgc tgctcgacat cgatttctcg
     2701 cgcgttgcct cggtcagccg cgcgctcggc gcaacgcccg agttcagcgc cacgcgtacc
     2761 gtggccggcg acgcgccgct cgccctcgcg gcgttgccgg cgggcaaggt gccgcgctac
     2821 cagggcgtgt tcgacgccgc ggcgcgcgcg atcctgactg ccgaaccgga ggacgtgcgc
     2881 aaggcggccg agctcgattt cacgggcgcg gccgaaacgg tgatcggcag tttcgacggg
     2941 ctgaatctca ccgtgcgggc gatcaaggcg aaggacggca actgggtggc gctcggcgcc
     3001 gccgcgggca ccccggtgcc gcgcgcggag gcgcccgcat cggagctggc cagcgacgag
     3061 gagctcacgc cggaaacagc cgggacagac gcgatacctg gcgaggcggc cgcgcaggcc
     3121 gagcgcctga accgccgcct cgacggctgg gccttcaagg tctcggacta cgtctacggc
     3181 gagctcgtca agggcctcgc cgattacctg caggacgagc agccgcctgc cgtggcgccg
     3241 ggtacgccag cacagccgcc agcagccggg caggcagggg agtcgcgcga tgaatgattc
     3301 cgacatcctc ggctcgcgcc gggcgatctt cgcgtggttg tggctgctga tcgggatggt
     3361 gttcggcatg gtcgtggtcg gcggcgtgac gcgcctgacc ggctccgggc tctcgatcgt
     3421 cgagtgggcg ccgatcatgg gcacgctgcc gccgctgaac cacgcggact ggctggcggc
     3481 gttcgcgaag taccagcagt atccgcagta ccgcatcacg catcccgaca tgacgctcgg
     3541 ggaattccag ggcatcttct tctgggaata cgtgcaccgc ctgctcgggc ggctcgtcgg
     3601 catggttttc ctgctgccgt ttctgtggtt ctgggcacgg ggctgcctga cgcatcgcct
     3661 gcgcgcacgc ctgctggtgg cgctcgggct cggcggcctg caaggcctca tgggctggct
     3721 catggtcaag agcgggctgg tcgatgagcc gcgcgtgagt catttccggc tggcggcgca
     3781 tctggggctc gcgttcacga tcatggccta cctgttctgg ctggtgctgc aactgcgtgg
     3841 cgatgatggc cgggcggcgg cgaaggccgc tgcgccgcgc gtgctcattg ccagcgtggc
     3901 cgcgctgacg gtactgcaga tcgtctacgg tgccttcgtc gccggcctgc gcgcggggct
     3961 cgtctacaac acctttccga agatgaacgg ccgctggatc gccgaaggcg tgatggggat
     4021 ggatcccgcg tggctcagcc tgcttgagca cgacgtcacg atccagttcg tgcaccgctg
     4081 gctcggcacg ctgctgctgg tgctggtcgc cgtactgtgg ctgcgcgtgc gcgccgggcg
     4141 tttcgcgctg caccagcgcg cgagcgccgc cgcgttgatg ggcctggtgt cgctgcagta
     4201 cgggctcggc gtctacacgc tgatcgagca ggtcccgatc gtgccggcgg tgatgcacca
     4261 gggctgcgcg agcctcgtgc tgctcgcgct ggtgtgggtg ctgcacggtt ttcgtcgcac
     4321 cacggcgtag agcgcgccgg tcagcggccc gccctggcgc gcagcaggta gcgcaacacc
     4381 cggtcgtcgc tgacggtcca gcgcagccgt ccccgcgcgg cgtccctgcc gcacagcagc
     4441 aggcgatcac ccatcgccag cgcttcctcg tcgccgggca gcaggctctt gccctcgcgc
     4501 cgctgcagca gcaggggcac ggcactcagc gggatttcgc ggttgtccgg tgctcgcatc
     4561 aggtcgccga cggtgacctt gcggccacga cgaatcgccg ccgccacggc cggcatggcg
     4621 gcggcatcga tgccgatcga ccaggtctcg gcaatctcgt caccgatgcg ctcgcgcatg
     4681 cgttccagca gttcgcgcac ccaggcctcg tcctgctggc gcgcgaggcg caggaagtac
     4741 gacagctgcg gggcgcggat gatgcgcagc acctcggcgg ccacgatgta gcccgacagc
     4801 gtggtgatgt cggcgtcgac cgcacggaac agcggcgtgt tgcgtcgctc cgactggcgc
     4861 aggaccatga aaaggcgcgg attcagctcg cgtgcgagca tcgcgatcgc gaggttggtg
     4921 gtgtcgttcg gtgttgccac gacgatggcg tcggcgtgct cgatgcgcgc gcggtgtagc
     4981 gtagcggcat cgatcgcgct gcccgtcacg tggtcgatgg tggcatcggc gggcgcgagc
     5041 aggtcgaccg ccgtgacctc gatgccgacc tgctgcaggt ggcggtgcac cgtgcgcccg
     5101 aagcgcccgt agccgcacac gagccagcgt ccgcgcggaa acgccggtgg ctcggccatc
     5161 ggcgtgctgg tctgcgtggt gaggctttcg tagatcacgt gcaggctcgg cttgatcagc
     5221 gcctgcgcga ggcgttcggc gaaggtatcg tgcggattga tcaggtgctc ggcgcccacg
     5281 cgcgccatcg cggcctggtg ctcgtggtga tgcgccacac agatcacctg gcgctcgggc
     5341 gcgaggatct tggcgttgag cgacaccgag aggttggccg cgtcgtcgcc ggtgagcgcc
     5401 agcgtggcga tgcaggccgg gtggttgacg ccggcggtga gcagcgtgcc cgggtgcatc
     5461 gcgttggcct gcagcgcggg gaccgacagc ggcagctcgt cggtctccac cgagtcgacg
     5521 cgctcctgca cgcgatcgac gaccaccacc cctatgcctt cctcgcacag ctcgcgcgca
     5581 atgagcttgc cggcatcgtc gtatccgcag agcagcacga agggctcgcg cagcccgcgc
     5641 accgcggcgg cgaagcggtt ctcgtgcagg atgcggcgaa acagcgggtc ctgcagcgtc
     5701 gagagcagcg cgccgatgcc gtagagccag gcggtgaccg tcgcgtagat cgaggcggtg
     5761 gcccacaggc gctgcgcgtc ggagaatggc tgcggtatct cgccgaggcc gatcgtggtg
     5821 ccgaggaagc tcacgaagta gaaggcgtgc aggaaaccca tgcgccaggg ttggcccgcg
     5881 gcgtcgattc ccggcaccag cgtgaagccg aacaccgcga cggcgtagac gacgatcagc
     5941 aggatcagtg gcaggcgcaa gcgcctgagc agcaggaaca gccccggctg ggtgcgccgt
     6001 acttccatgg tcatcggcgc aggctggcgg tctctatcat cagcagcacc accgatatca
     6061 cgttcgccac catcgcgccg atggccagcg acacgacgat gaccgtgtcg tgctcggaaa
     6121 tggccgcacc gtcgagcgtg gcgctcgccc ataccacggc ggccgcgatc agctgcacgt
     6181 cggcgaccag gctggtcgcc agcatcgtcg agccgagcag cgagcggtcg ccgaacttca
     6241 tcaccgtggc gattaggctc acgacgatcg ccagcgcgag ctcgtacttg tcgtggtgcg
     6301 cgatgtcgtc gatgtcgccg accacgaagc ccacgttcag cgtgagcgcc agcacgatca
     6361 cgaacgcaaa caggatcttg tcgaaactca tcggcctctc cgtgcggtgc cagtcagcac
     6421 ggcgaggcta accgggaacg cggcgccggg caacggtgcg gcaactctca ttcgctgcgc
     6481 agcgcctcga tcggatcaag gtttgccgca ttgagcgcgg gaacgatgcc ggcgagcagc
     6541 ccgatgccgc aactgagcgc cagcgacagc agcaggtaga gcggttcgat cgcgatcggc
     6601 agcccgggca gggcggcgcg cccgagcagc accagggcga gcaccgcgag gagccccagc
     6661 gccccgccca gggccgccag cagcacggct tcgccgagaa acaacagcag cacctggcgg
     6721 cgcgtggccc cgagcgcgcg cagcaggccg atttccccga tccgctcgcc gagcgccgtg
     6781 ctcatgatcg tgagcacgcc cacgccgccg accaccagcg ccacgctgcc gatcgcgccg
     6841 atcgcgaact tcagcatcgt gaggatcttg tccaggctcg cgagcatgtc ttcctgcgtg
     6901 aagagcgtga agtcctcctc gccgtggcgc tcgacgaggt gcgcgcggat gcgctcgctg
     6961 tactgcttcg aggtggtcga ggcgcggaaa accaggtcga tctccatcaa tccctcgcgg
     7021 ttgaacatcg cctggccgag gtcggtcggg atgtagatcg cgtcgtcgag atcgaagccg
     7081 agcaactgtc ccttggactc catgacgccg atgacctgga agcggctgcc gccgatgcgc
     7141 acgtactcgc ccagcgcgtt gcgtccctgg aagagttcct ccttcacttt cgcgcccagc
     7201 gccgcgaaat agcgcgaatt gcgcagcccg tcgtcgggca ggaaccgccc ctgggcgacg
     7261 ccgaagcgcc acgccgccgg catgtctggc cccacggcat agacgtcggt gtcgcgctgg
     7321 tagcgcccgg cctcgatctt gctcgtgccg agtatcacgg gtaccacggc ctcggtgtgc
     7381 gccaggcgct gcagcgagag cgcgtcctcg agcgacagcg ggcgcaccgt ggcgagcatg
     7441 cccgcgccgc ccagggtctc ggtgcgcccg ggcgtcacgg tgacgatgcg ggtgccgaac
     7501 tgcgagaaac tgtcgagcag gtagacccgg atgccttcgc cgatcgaggt gagcagcgtc
     7561 acggcgcaga tgccgatggc gatgccggtg gcggtcagca ggctgcgcat gcggctgctc
     7621 tccagcgcgc gcaggatcca gcgggtccag tcgagcgcgt gcatcgcctc agcgcctccc
     7681 gagcgcgagc acgggttcga gccgcgcggc cttcccggcg ggcagccacg cgaacagcag
     7741 cgcggtcgcg agtgccagcg cgctggccgc gaccagcgcc cagagcggcg aggcgaaggg
     7801 gatggcaggg tagagcgccg cgatcagcgc gatggcggcg cgaccgccca gcatccccgc
     7861 gatcgcgccg gcggcggcga gcacccccgc ctcggccagg aatatcgcgc gcacctgcgc
     7921 gtcggttgcg cccagcgcct tcagcagccc gatttccggg gtgcgctggc tgaccgacat
     7981 cagcgtgagg ttcatcacca ggatgccggc cacgaccagg ctgatcgcgg caatgccgct
     8041 gacgccgagc gtgagcacgc gcagcacacc gtcgaaggtg ctgagcatcg cgtccgggct
     8101 ggtcacggtc acgtcctcct caccctcgtg cagttccgtc atgcgcgcga gcagcgcctc
     8161 cttcagcggc tcgatcgcca cgccctcgcg cgccttcagt accacccgga acaggccgtt
     8221 ggtgttgaac accgcctgcg ccgaagccac cgggatgaaa atcgcctcgc tcagatccat
     8281 gccgaaggaa tcgccgcggc cctcgagcac gccgatgacg cgaaagcgat agtcgcgcag
     8341 ccgcacccac tggccgatcg cgcggcggtt gccgaacagc tcgcccttca atttctgccc
     8401 gatcacggcc accggcgtgc cgatgtcgag tgcgagcgag ggcagcatgc tgccctgggc
     8461 gatgcgcagc ttgcgcaccg cgaagaaatc ggcgctggtg ccgatcacgg tgctgtcgcg
     8521 ctcccgggcg ccgtgcgaga ccggcgcgtt gccgaccacc accggtgcaa tggactcgat
     8581 gcccgcgacg gtgcgctgga tcgtgagcat gtcggccagc gtgatgtcgc gcgcggcgac
     8641 cccggtgacc ggcggcatgc cgcccgtggt gctcttgcgc ccgggaaaca gcaccacggt
     8701 gtccttgccg atgaaagaga actcgcccag gacgtagcgg cgcgcgccct cgccgagcgc
     8761 gaccagcacc accaccgagg ccacgccgat cgccaccgac agcagctgca tcaggctgcg
     8821 aaagcgctgg cgccgcaacg cgagcagcgt gaagcgcagc aggtcagcgg ggcgcatcgg
     8881 ggctctgcgc gcgcgccgcg ctccccgggt tcccggcggg cgcgtcgcca ctgatcgcac
     8941 cgtcgagcat gctgaggtgg cgcgccgcac gccgccccag cgtggcgtcg tgggtcacga
     9001 ccagcagcgt gatgccatcg cggttcagtt cctcgagcaa cgcggtcacc tcggcgccgg
     9061 cgcggctgtc gaggttgccg gtgggctcgt cggcgagcag caggtcgggc ttcatcacga
     9121 tgctgcgccc gatggcgacg cgctggcgct cgccgccgga gagctggttc ggcaggtggc
     9181 cggcgcgctc cccgatgccg aggcgctcga gcaccgcgtc gacggcgcgc aggcgctcgc
     9241 gcggcgccct gccggccagc atcagcggca gctcgatgtt ctcgcgcgcc gtgagacgcg
     9301 tcaccaggtg ataggactgg aacacgaaac ccacgtgctc gcggcgcagc cgcgcgcggg
     9361 cttcctcgcc aagcgcggcc gtgggcgtac cgttcagcag gtattcgccg ccgtcggggc
     9421 ggtcgagcag gccgaggatg ttgagcaggg tcgacttgcc ggagccggac gggcccatca
     9481 ccgagacgta ttcgccgcgc ccgatgcgca ggttcacgtc gcgcagcgcg cgcacctcgc
     9541 tgtcgcccag cgtgaaggtg cggctgagcg cgcgcagctc aatcatcggc gggcgtcgcc
     9601 agcacgccct cggcgaggcc ctgctggtcg agcgaggtca cgacgcgatc gcccggcgcg
     9661 agcccggcgg tgatctcggt aaagttccag ttgcggatgc cggtctgcac cgcgacacgc
     9721 tcgagcctgc tgctggccgg gtcgaagcga tagacagtgt cgttgtccat cagcgcctcg
     9781 gagggaatgc gaatggcatc cgcgtgcgct tcgagcacca ggtcgatgtc ggcactgtag
     9841 cccaccagca ggcgctcgcg gtcggcggcg ttggtgaaca gcacgtccac atcgacggtg
     9901 cgtgcctgtt tctcgatctc catcacgtag ggcgcgatgc gatcgaggcg gcccgcgaaa
     9961 tgctcgccgc gaaaggcatc gaggctgatg cgcgcgggca ggccggcctt caggcgcccc
    10021 gcatcgacct cgtcgatggg cgccgtgacg tacaggcagg aataatcgat gagatccacg
    10081 gccggcggca tgggaatgcc gggcggcgag ggcgtcacga actcgcccac ctcgccgttg
    10141 atctccgcga cgatgccggc gaagggggcg cgcagctggg tctcggcgag cagcgcgcgg
    10201 ttcatctgca ggctggcggc cgcggtctgc tgttcgtcgc cggcggcgcg gcaggcgaag
    10261 gcggcggaac gcgcgcgcgt atcagcggct tgcgcgagtt cctcggaaac cagcttgcgt
    10321 tgctgcagcg tggcgaggcg acgcgcttcg cgcgcggcgt tgtcggcttc cacgcagagc
    10381 tgctcgcgcc ggtgcgaggc cgcggcgagc tgcgattcgg cctgtgccgt catcgcgcgg
    10441 cggtcctcgt tccacagctc cagcagcaac tcgccggcgg ccacctcctg gccctccttc
    10501 acgtgcagca cctcgacccg cccgccgccg ggcatcgaca gcgcggagcg gcggcaggcc
    10561 ttgatggtgc ccgcgcgcgt gttcgcgacc gtgcgctcga ccacgccgcg ctccacggtg
    10621 tgcagcgtca cgggcagggg ctcctcgcgc cgtgtatacc agatagcgcc gagcagcagc
    10681 gcggctgcca ggatgaggac caggattcgt ttcatcgcta aatgcgctgc catgctccgt
    10741 cgcgcacagg tgggctccgg cgcgattatg gcacaatgcc gccgggctca aggaggcggg
    10801 aacgacaatg gaaatcgcgg gaaaaacggt actgatcacg ggcggcagcg cgggcgtggg
    10861 tctcgccgcg gcgcagcgct tcctgcagtt cggcgccagt gtcgcgatca ccggccgcga
    10921 tggcgcgcgg ctcgcgcaag ccgcagcgca gctcggcgcc ggtgaccgcg tgatcaccat
    10981 cgcgtgggac gctgtcgaga tagcgcgcgc cgggacggtg ttcgccgagg tcgagcggcg
    11041 ctttggcgcg ctgcacgtgc tggtgaacaa tgccggctgc aatcaccgcg gcttgctgga
    11101 ggagctgtcg gaggaggagt tcctgcacgt cgtcgacacc aacctgcgcg cgccgctcgc
    11161 gctctcgcgc acggtgttgc cgttcatgcg ccgggccggg cagggcgcga tcgtgaacgt
    11221 cgcctcgctg gccgggcggg ttccgctggc gcacgagtcg atctacagcg ccaccaagtt
    11281 cgggctgcgc gccttcacgc tggcgattgc ccaggagctg gagggcagcg ggatcagtgc
    11341 gtccgtggtg tcaccgggtc cgatcagcac gggattcatc ctcgacgaca tcgacggcgt
    11401 gcccgacatg gtgttctcgc agcccatgtc gacggccgag gaggtggcgg atctgatcgt
    11461 gcgctgcgcg cgcgacgggc gcgccgagcg cgcgctgccg atgatctccg gctggctggc
    11521 caacgtggcc tacctgctgc cgccgctggc gcgtgtgctg cgcccggcga tggagggccg
    11581 cggacggcgg gcgaaggcag cctatcgtgc acgacgctcg gggagctgac acgaatggat
    11641 gccatgcgtt tggtggagat cagcgccttc ggcgggccgc gggtgctgcg cgagacccgc
    11701 ggtccgctcc cggtgccgca ggccggcgag gtgctggtca gggtagcgta cgcgggcgtg
    11761 aaccgcccgg acgtggcgca gcgccagggc gtctatccgc cgccgccggg cgcatctccg
    11821 gtgccggggc tggagatcgc cgggcaggtg gtggccacgg ggccgggctg cgcgcgctgg
    11881 cgcgtgggcg acgcggtgtg cgcgctcgtt gccggtggcg gctacgccga atacgcgacg
    11941 gcgccggaag cgcaatgcct gccgttgccg cgcgggctgt cggtgtgcga ggccgccgca
    12001 ctgcccgaga ccgcgttcac cgtgtggagc aacgtgttcg agcgcggggc gctcgcgcgc
    12061 ggcgaactgc tgctggtgca cggcggcacc agtggcatcg gtacgctggc gattcaactc
    12121 gcgaaggcac gcggcgcgcg cgtgatcgcg accgccggca gcgaggacaa gtgcgcggcc
    12181 tgtcgcgaac tcggtgcgga cctcgcggtg aactaccgcg aaacggattt cgtcacggcg
    12241 gtgaaggaat tcagcgccgg gcgcggcgcc gacgtgattc tcgacatggt cggcggcgat
    12301 tacacgcagc gcaacatccg cgccgccgcc gagcaggggc gcatcgtgca gatcgccttc
    12361 ctgcagggct cgaaggcgga actcgacctg atgccgctga tgctcaagcg cctggtgctc
    12421 accggctcca cgctgcgcgc gcggtcgctg gacttcaagg cagcgctcgc gcgagccgtg
    12481 gagcagaacg tgtggccact ggtggagagc gggcgggtgc gtccgcgcgt cgcgcgtgtg
    12541 ttcgggctcg acgaggcggg cgccgcgcat gcgctgatgg agtccagcgc gcacatcggc
    12601 aagatcgtgc tgcggatcga ttgagccggc gggtgcgcct tcccgggtcc gggaaggcgc
    12661 tctgcgcggc gctgcgatta ccagccgtcg ctggccttgg ccggttgctc cggcgtggca
    12721 gcggccggcg cgccccgcgt cacggtgccg gcgcgcatcg cctcgaccga gacatccgcg
    12781 ggtgtcgcag ccggtgattc cggtgccacc aggtgctgca gttcgccctg tttgcggatt
    12841 gcctcgcggt agatggcgtc cttgtcgatc agcggcgcca cgtcgggttc gggcggacac
    12901 acggctgcca tgtccggtgc ctcctcgtcc gtgcccggtc gttccatggg gtgcaggtcg
    12961 ttgccgagct ccagcgcgct gacgagcgcg ccggtgccgg ccagcgcgcg cgtgtaggag
    13021 atggcgttca gccgcctggc gttcacgtag gctcgccgcg acatgaagta ctcgttctcg
    13081 gtgtcgagca tgtcgagcag ggtgcgctgg ccgatatcga actggcgccg gtaggccatg
    13141 cgagcctttt ctatggacgt ctggtgacgg tccaggaaga ccagctgctc gtcggtcgca
    13201 cgcatttcgt tgtaggcgat cgagacttcc tggcggacgt tgcggcaggt catcgtgcgc
    13261 atgtcctccg cctggttggc cttgtagcgc aggcgcctgc gctcggcgag gtccgaaccg
    13321 ccgttgtaca ggttgtaggt catcgccagc tcgatgacgc cctcctcgtg gcgcccgttg
    13381 atgtcgtcct tgtcgttcca gatgtcctgg cgcgcgcgca cgtcgaagcg cggcagcatc
    13441 gtggccttct gcacgtcggc ggcgctgttg gcggaccaga cgttggcgat catcgcattc
    13501 atctgcgggt tcgaggcgta ggcgagtgct agcgtgtcgt tgacggtgcc cggcagcagg
    13561 ttcggcgcga gctcgggttc ctgcagcgat tccgcgggcg tctcgccgac gatgcgctgg
    13621 tagcgcatgc tggcatcgtg caggttcgtg acgtccgtca gcaggttcga ctccgagagc
    13681 gcaaggcggc cgaaggcctg ctccaggtcg acgccgcggc tgacgcccgc cctggtgcga
    13741 cgctcgatct gctcgtagat caggcggtgg gcggcgtagt tgtccttcga cagttcgacc
    13801 agctcgcgat ggcgcagcac gtcgtagtac gcgcgggcca cctccgcgct gagccgttcc
    13861 gaggcatcca gaacctcgaa atagcgcatg cgcgccgcga agtcgaagct gcgcacgcga
    13921 ttgcgcgtgg cgaaaccgtc gtagatcatc tggttcagca gcagcgacac gcggtcgcgg
    13981 gtgaagtcgt actcgtcaaa gcgcggctcg tcgatgcgct ccgccccgac agctgcctcc
    14041 aggtccacgc gcggcagata gcctcccttg gctacgcgcg tttgctgctc ggccgcctgg
    14101 aactcgtgga aggcggagag tacctcgggg ttgttcagca cggtctgtcg agccgcgtcg
    14161 cgcagggtgt tgagtgcgcc gccgtgcgcg gcggccgtga cggccaggca cgcgatggcc
    14221 atcgaaatgc ggtatcggct gctcttcatc agggtcccgt gtggtttcga atcggagggg
    14281 caaggagaaa cagtcttcat tctaggcact atatcgagcc tccagcgggc aatccagccg
    14341 acgaaatgcc gccttgctgc tactgtttga cctgcatcac agcggagcgg gcggacacgg
    14401 tgacggggcg ggacggaagc ggcgggccgt gggcggcgga ttacccgtgc gtttgaacgc
    14461 gacggtgctg gcgcgccgcg tcctcggtgc gctgctcgcg gtgctcgggc tgatcggggc
    14521 gtgcctggcg aacccggatc tggaccggat cacgcagctc gcactggagc gctacggcgt
    14581 ggatgccggc gagtcggtag ggctgtggcg cgcgctcatc gaggagaacc gcgacctggc
    14641 ggagcgcgag aagctcgagc tcgtgaacac ctttttcaac caccgcgtgg atttcgaaca
    14701 ggatgccgtg gtctggggtg tgcgggatta ctgggccacc ccgctcgaga cgctgggacg
    14761 cgggcagggc gactgcgagg atttcagcat cgccaagtac gtgacgctcg gtctgctcgg
    14821 cgtgccgctc gaacgcctgc gcatcaccta tgtgcgcgcc gagaccgggc tcccgggcag
    14881 cggcatcgtc caggctcaca tggtgctggc ctactacccg gagcccggcg tggagccgct
    14941 gatcctcgat aacctcgtcg gccagcttcg cccggcctcg cgccgtcccg acctgaagcc
    15001 ggtgttcggt ttcaacagcc agggactctg ggtcgccggc aagaaagcgt cggcggaccc
    15061 gacggcgcga ttgtcgcgat ggcgcgatct gctgcgccgg atgaacgaag agggcctaaa
    15121 ctgatggccg cgtcgcaatt ccgtcaaccg gtcagttcgt gggagccgtc aacatgtcgc
    15181 tcgtaaagca gctctggctg gcgattgcgc tggtgatgct gatcgccttt ggcggcagtt
    15241 tcctcgtcag cacgctgtcg gccagaaatt acctcgagga gcagctcagg ctgaagaatt
    15301 tcgacaatgc gacttcactg gcggccacct tgtcgcaaac actggagcgg acgcccggag
    15361 acgaggtcac catcgagctg cagattgccg cgcaattcga cctcgggcat tacgagagca
    15421 tccgtctcgt cgatccctcg ggcaaggcgc tggtggaatt cgcgagcgat gccggcgtgg
    15481 acggcgctcc gcagtggttc gcgcgcttgt tccgcatcga ggccgaaccg ggcctggcgc
    15541 aggtgcagaa cggctggcaa ccctacggga cgctgagcgt cagcagccag gccagcttcg
    15601 cgtatcgcga actctggaac agcatgttgc gcctgctggg ctggttcctc gccgccgcag
    15661 tgctcaccgg cgtggccggc accgtgctgc tgcgcctcat cctgcgtccg ctcggcgcgg
    15721 tggtggacca ggcggaggct atcggcgcac ggcgcttcat caccaccgcg gagccggcga
    15781 cccgggagtt ccgcgccgtg gtgcgctcca tgaacgcact ggcgacgcgc gtgagacaga
    15841 tgctccagga cgaatcctcg cgtctcgacc agttgcgccg cgaggcacac cacgacgccg
    15901 tcaccggcct gctcaaccgc agccacttcc tggcacgggt gcagagcgcg ctggcgcgcg
    15961 aggatgccgg cgccgagggc gtgctggtca tcgcccggct gctcgatctg cacgagctga
    16021 accgggagca gggttgggcc gtgatggaca cgctgatcaa gcgcttcgcc gaggcgctgc
    16081 gcgccatcgc gccgaaggac gcggagtgga tcttcgggcg cctgaacggt tccgatttcg
    16141 ccgtgctggc tcccggggag gacgatgccg gtgcgctggc gcaacgggtg caggaggcgc
    16201 tgcagatgct ggcccgcgaa ctcgacctgc aggccgtctg tcggttaccg gcagcggcga
    16261 caccctaccg ccacggcgac cagctctcgc gagtgctcgc gcgcgtcgac gtggcgctgg
    16321 cgaatgccgt cgcggcgggc ggtgacacgg tgcaggtggc gggagtggaa caatcgccac
    16381 ccgacgccgg gatgcgcatg gatctcgtga cctggcgcca gcgcttcgac tcggcactca
    16441 ccatgggcca ggtgaaactg cacagctacc cggtggtcga tgccgccgga cagttgctgc
    16501 atgacgaatg cgtcgcgcga ttgcgactgg atgtggatag tgactggctg aacgcgggtg
    16561 agttcgttcc ctggctcgcg cgcctgggcg atctggcgcg gctggacgag atggtcctcg
    16621 atcttgcgat cgcgcggctg ctcaactgca ccgacgacct gtgcatgaac ttctcggcgc
    16681 aatcgatgaa cgatgccgtg ctgatgcatc gcctggcatc gcgtcttggc gagacgcccg
    16741 aactggcgaa acggctctgg ctggaagttc ccgaatacgg cgtgttccag aacctcgaga
    16801 atttccgcat tctcagcgcg ctgctcaagc ccctgggatg ccgcataggg atcgagcacg
    16861 tgggacacca ggtcgcgcgt atcggcgaat tgcacgacct gggactcgac tatctgaaaa
    16921 tcgatgcgtc cttcgtgcac ggcatcgacc agaaccaggc caaccaggtt tttctccgcg
    16981 ggctcgcgat gatcgcgcac tcgatcggtc tcagcgcgat ggccgaggga gtgggcaccg
    17041 aggcagagtt ccgcgcgctg gtcgaactcg gcttcgatgg tgccaccggg cctgccgtta
    17101 ccatgcgcgc cggctgagcc ccctacagca ggctggtatc gtcgcccagc agcccgaggt
    17161 gcgaaccggt ctcgcgcagg ccgcgccgct cgatcagctt gcgctgcgcc gccgccggca
    17221 ggtcggtgaa gcggatcagg ttcttgtcga tgagcaggtt caccaggtca tccagaaccc
    17281 ggaccacgtc gcggtctgat tcctgcaacc gcgctttcgc gtcgccgagc cgatgctcga
    17341 actcggcgat ttcggccgcg tccagcagcg cttcctcgaa tccctccagc gcttcgctgc
    17401 tcagcgcgat gagctgtccg gtcgcgtctc gtcgcacata gggcatcgcc agttctcctc
    17461 ggccggggcc ctgcccggcg ccaggccgga caggggcgca gcccgcctca ctcggtgatc
    17521 agcttgccgt ggttgatcag catgtcgatg atctgctgat cgttgccgat gctcagcagg
    17581 tcgacgttct gcagttcgat cgtctggtag gccacgttcg cgtcgtgggc ggcgtcgccg
    17641 gtgaagtcgc ccgcggggct gatcttcacc agggtgccac cagtgcccga gtcctcgaag
    17701 tgcagataac tgtcgagact tgcagcgctc tcgggcggca gcagatccct gaggtcgagc
    17761 acgtcgccac cgcttgccgg ctcgttcatg tcgaagttgc tgatcgtgtc gtgagctccg
    17821 ggttcagcca gcgtccactt gaacacgtct gcgccctcgc tgccccccag caggctgtca
    17881 ccgctgtcgt ccggcagcac gtcaggatcc gcaccagtcg catcgtcctt gccggaggcc
    17941 cggtggccgc gctccccggg tttgtcgtcc ttcgcctggc ggttgccgtc atcgatgacg
    18001 gtggtgtcga gtacgtcgct catgctcagc gccgccgctg gcggcgccag ctcgatcgga
    18061 tcgaggctcg cgatctccgt cgggctgata ttcaccgaac cgtcataggt gcccgacgcc
    18121 agtgcggcgt tgccccccgt gttctggatg aggtcggtga aggtggtgcc gaaggtgtga
    18181 atccccagcc ccgccttgat ggcatatccg acgaccgagt agccctcgag gaggggatcg
    18241 gcattcacgg cggccgccac cgcagcggcc agggtggccg gctgcgcgta gtcattgatg
    18301 ttcaatgatg agctgtagtt cagcttgacc gccaccaggt tgttcccgct ggtcaggtag
    18361 aacaccacat tggagatgtc cttgggcgtt ggcgtgggtg ccggcgttgg agtcggcgtc
    18421 ggttccggag tgggttccgg tgttggtgca ggcgtcggct ccggagtggg ttccggagtg
    18481 ggttccgggg tcggagccgg agtcggcgcg ggcgttggtg ccggcgt
//
LOCUS       DAORUQ010000044        18401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2676_length_18401_cov_7.213125, whole genome shotgun sequence.
ACCESSION   DAORUQ010000044 DAORUQ010000000
VERSION     DAORUQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18401
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_18401_cov_7.213125"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..614)
                     /locus_tag="PLW13_05845"
     CDS             complement(114..614)
                     /locus_tag="PLW13_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HQY69929.1"
                     /translation="MLPAWIRSRQVFLGIFIATALMMGFGYWLQYAEGLEPCPLCMTQ
                     RIFITLAGLTALVAALHGPRRAGIRVYGSLVTLWCLVGGGFSLRHLWLQSLPPGQAPA
                     CGPDLAYMFDAFPLKDALLLLLRGDGNCAEVDWAFLGVAIPGWTLLAFAVIATLAAWS
                     AFRPRT"
     gene            733..1164
                     /locus_tag="PLW13_05850"
     CDS             733..1164
                     /locus_tag="PLW13_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HQY69930.1"
                     /translation="MSIARRACLMLLCLLPLMAQAKSAEKAEEGGPDFEYIEMQPAFV
                     VNFGTSGRIGYLKTEVSLRVGAEAVAAVELHMPALRHELIMLLSGQSAEALAAPEQRE
                     ALRLAALEAVRRVIAAAAAGDAAPAQSGVKDLLFTSFILQR"
     gene            complement(1356..1940)
                     /locus_tag="PLW13_05855"
     CDS             complement(1356..1940)
                     /locus_tag="PLW13_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HQY69931.1"
                     /translation="MNHLAHFHLAGECEHLVVGALLGDYVKGPLTGALPQALERGVRL
                     HRRVDAFTDGHERLRALRVHFGPGERRLAGVVIDLFFDYLLTRHWERFHAPALREFST
                     RVCIILERHAAALPPPAARQARRIVEHDLLCRYGEVEVLEGTLERLGEVLRQPQAMRA
                     ATAVAWSRIGEFEESFLAFYPQLVEMAGAPEFRG"
     gene            complement(1950..3518)
                     /gene="gshA"
                     /locus_tag="PLW13_05860"
     CDS             complement(1950..3518)
                     /gene="gshA"
                     /locus_tag="PLW13_05860"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQY69932.1"
                     /translation="MNAQFEHRLAALDRGDNSRLLRYIRRGIEKESLRITPQGRLAQT
                     PHPAALGSALTHASITTDYSEALLEFITDAGTELEQTLDELDRIHRFVYPRIGDELLW
                     TSSMPCVLEKDEDIPVARYGDSNVARMKTIYRIGLGHRYGRLMQTIAGLHYNFSLDDD
                     FWEWLRAETANTLTLQDFKTEQYFALIRNFHRHAWLLVYLFGASPALCGSFVAGRAHG
                     LQPLGAGTLHAPWGTSLRMGDLGYQSNAQRSIDIPYNSLGDYVGALQRAITEAHPDYQ
                     RLGVRDGDQWRQLSASLLQIENEFYATIRPKRVTRSGESPRRALQERGVEYIEVRCLD
                     LDPFEPIGIARPTARFLDAFLLFCLLHQSPASDAPARRLARENLLTVVNRGREPGLHL
                     AQADGASVELRQLGLRMLEDIAAIGVLLDRARNGVEYRDSIAAQRLKLEQSGLTPSAR
                     VLEALRAGEGSFFRFAMAQSRLHREHFLARPLDDVSRAQFERLAVESLDAQRAIERAD
                     TIPFETYLANYYAQ"
     gene            3689..4591
                     /locus_tag="PLW13_05865"
     CDS             3689..4591
                     /locus_tag="PLW13_05865"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HQY69933.1"
                     /translation="MPTPQPAIFRENTPHQYFLEFHLRARWQPEELRRLLGQALAARA
                     PETSLVLAFGRDAWERLAPQEVPENFITFHPVLGRQDRIAPGTQGDILVWLHGERHDL
                     NVALAMEIGRALAGIATLELELHGFTYLDSRDMTGFVDGTENPHDEEARAAALIPAGH
                     TGAGGSFVLTQQWVHDLERFGALGQAEQESVIGRTKPDSIELDDAPPTAHVRRTDVQV
                     DGQALKIYRRSAPFGGLRQHGLYFIAFSREIMIFAMQLDRMFGRADDGLMDRLTEFST
                     PVSGAYWFAPPVESLTALFGDHAG"
     gene            4658..5737
                     /locus_tag="PLW13_05870"
     CDS             4658..5737
                     /locus_tag="PLW13_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY69934.1"
                     /translation="MRRLTRNAMLIALAGATAFAGWQLLRGRGAAVEAFEPPAPAVRA
                     ATVERREIADRVEALGTTSSWESIELRPTVTEFVSAIHFTDGQAVTRGDALVTLAQQE
                     ESAQLDEARAYLDEQLREVRRIEGLVARKSLPQSQLDERRTLRDIALARVSAAQAALA
                     DRTIRAPFTGLLGLRNVSPGALVTPETVITTLDDVATLRLDFTVPSALLSRLRTGEAV
                     TAASPAFEGREFSATVTGIDSRVNPVDRSLTVRARIDNADLALRPGMLLTLALEHHPR
                     TALVVPEEALVHYQRSHFVLVVDGADGNRLVRREVTLGTRIPGSAEILSGLEEGELVV
                     VEGLTTARPGQQVRILAPQGVESPP"
     gene            5734..8853
                     /locus_tag="PLW13_05875"
     CDS             5734..8853
                     /locus_tag="PLW13_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY69935.1"
                     /translation="MMLSDLSVRRPVLAAVLSLLLIAFGAVAFDLLPLREYPDIDPPV
                     VTIETTYRGASAAVVETRITQPLEERIAGIAGLEAISSGSEDGVSLIQLEFSLERDID
                     DAANDVRDRVGGALDQLPAEADAPDIRKEDASGRVMLWLNLVSDRMDRLELTDYANRF
                     IADRFAVIDGVARVQVSGGLDYAMRIWLDRDALAARALTVADVEAALARENLELPAGA
                     IRSEQRDFVVRMERSYHTAEDFRRLVLGKGGDGHLVRLGEVATVELGAAENRRQFRGN
                     GVPMVGIGIVKQSNANTLAVARAVRAERDRTNLSLPEGMELKQSYDSSVFIESAIAEV
                     YFTLFIACGLVMVVIWLFLGDTRAMLIPAATLPVSLIATFIVLYFFGYSINLLTLLAL
                     VLAIGLVVDDAIVVLENIHRRMRAGETPLVAAWRGTRQVGFAVVATTLVLVSVFVPIT
                     FLQDDIGRLFGEFAVAMAAAVLFSALVALTLSPAMCSIMLRPDSAEHGAGRAFTRAQA
                     AYRRSLAGVLHHPWIALAVFAAVLAGAWLLMRAVPSEFAPREDRGTFFMFITAPEGSS
                     FDYTMRHVDDIERRIMPMVERGEIERMLLRAPAGSTRTETYSAATGIFNLPHWDTGRP
                     PAWETIAEVSRRIADVPGVQAFPTMPQGLGGRFEKPLQFVIGGGTFEELAQWRDILLE
                     RIAQNPGLTAVDHDYKETKPQLRIEVDRARAGDLGVSVENVGRTLETLLGSRRVTKLL
                     MNGEEYDVILEAIEDTKRSRADISNIYVRSATSGALVPLASLVRTEESADAGALNRYN
                     RTRAITIEANLADGYTLGEAIDFMENAARELLPPRATTDLKGESRDLRESGRSIYLMF
                     LLSLLVVYLVLSAQFESFLHPLIIMLTVPLAVVGALLGLWLTGQTLNIYSQIAIIMLV
                     GLAAKNGILIVEFANQLRAEGLEREEALLEASSLRLRPIIMTSITTIMGAVPLVLSGG
                     AGAETRFVIGVVVIAGVLVSTLLSLIVVPLTYHWLTARAPLPGATGQRLERELAAHGA
                     SEARAGSGM"
     gene            complement(8915..9925)
                     /locus_tag="PLW13_05880"
     CDS             complement(8915..9925)
                     /locus_tag="PLW13_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY69936.1"
                     /translation="MPPRFRTAITLPGSSGNVADTLALARRAESAGYDDLWLADAGGM
                     DALTLAALIAQNTARARIGIAVVPAYTRTPAVLASTAATIAQVAPGRFVLGLGTSSHA
                     IIEGWHGLELAQPLTRMRETVQLLRQMLAGEKTRFSGETLKSHGYRQEAVAGGLPIHL
                     AALRPRMIELAAELGDGVILNLFPRRALPRIVEHVARGARRAGKDPAEVEIVCRHQIA
                     VTGDPAATRKLFRMVFASYYATPVYNEFLAWAGYEHAAREIREGWAAGDRERTAAAIS
                     DELVDEIAIIGTREQCQQRVLDYARGGIHTHVVSCLGFEPDVVDATYRAFAPEHFGFR
                     GL"
     gene            10136..11785
                     /locus_tag="PLW13_05885"
     CDS             10136..11785
                     /locus_tag="PLW13_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY69937.1"
                     /translation="MKTYAYPLQLLADHVARHPERPYLHQPVDRVWRTFTWRDVDAQA
                     RRLANGLLAQGYTAGDRIAILSKNCAEWFIADFAIMMAGLVSVPIYPTASAATIRYVL
                     EHSEARAIFLGKLDATAPADEAIGPEVLRIALPYPTAPAAAHWNDWLARHQPLAETAQ
                     QRPDDIFTLVYTSGSTGVPKGVVISHRNMAASAQSTRDNVKARPGDRLLSYLPLAHIA
                     ERAVVEQLSLGLDVEVYFVESLDTFAEDLRHARPTAFMSVPRLWTRFQSQILRRMPDA
                     RLQRLLAIPLVGRVVARRIRKAMGLDSARLFGSGTAPISPAMLAWFARLGIPISEGWG
                     MTETTGLSCSNYPYRPDWVGTIGKPLDCVEMTLSPAGEILVRGDAVFREYYRNPEATA
                     ATFRDGWLLTGDIGEQRPDGAFRIIGRVKEQFKTAKGKYVAPVPIESLLAANPVIDQV
                     CVMGSGRKQPVALAVLAEGEPRSEAELARELESLLEQVNGQLESHEALDHIVVCAEPW
                     SIENELLTPTLKLKRERIEARYGPLLESTPLHQPVVWEQLR"
     gene            11866..13557
                     /locus_tag="PLW13_05890"
     CDS             11866..13557
                     /locus_tag="PLW13_05890"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HQY69938.1"
                     /translation="MKALLRCLLSLLLLTSYGCAAQPREEAPLFSQAELDQMLAPIAL
                     YPDQLLSQLLMAATYPLEVVQAARWSREHPGLKGPEAVRAVEDRAWDPSVKSLVAFPD
                     LLAQMDADLDWTERLGDAFLAQQQQVMDTVQSLRQRAYDAGNLRSGEQVRTVRERELI
                     YVRPAYPDVIYVPYYDPFTIYGSWWWPDYRPVYWAPWPSYGVSLGYASHGGFYWGAGY
                     NLGVGFFFSDWDWHRHHVRVVDYRPFYYRRPPYAQHIWVHDYSHRRGVPYRHRAVEHR
                     YVPVDSRPVRRHDYGDYRDSDRDYRYRDGERRDDRRSQPGQEPGREFNRAPERNRDRS
                     FNTDRATTGPATIEPRTVAPGENGVRRDNGMRRETMPQPADRAATPPERYREARGDAS
                     SRPDRIREPRDSATSRPERIREPRDTATARPESIREPWPGEPGMQRRTATPRASEAPL
                     QRPATPREVQRYPAPRNVQREMPERVQPEALPRARSYPQAAPAREPRSDGGGFVAPDR
                     GTREPLRSAPPKGRSDMSQERGAQRSDSAGGFPRGDRGDHGDRGTRAGRGFRDQP"
     gene            complement(13563..14957)
                     /locus_tag="PLW13_05895"
     CDS             complement(13563..14957)
                     /locus_tag="PLW13_05895"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY69939.1"
                     /translation="MESTLTDFVRVLRNAEIRVSPAETMDAARALELVGYADRERVRL
                     ALAATLAKTIDDKTLFDECFERFFAWRRPPEAGQAQDALAAVIPPRADVAAPGASTAQ
                     GAMAGELSELAQLLLAGDAAGLRIAIDAAGRAAGIGDMKVFTQRGLYSRRVLEQLGWP
                     QLQAEILGLEQAPPEAEPGHAAGIALKRGAEMLRDRVREYVEDQYLLFASANARELRE
                     SVLRSARLSQLERRDLQQINRLVRRIARRLSARYARRRRTARRGLLDVPKTLRKGAAH
                     DGLLFEPAWKSVRKDRPALIAVCDISGSVSAYARFLLLFLYSLSDVLPRVRSFVFSSQ
                     LAEVTPLFEDNPPEVAVELAQRQYGGGSTDYAAAIRGLLAATGRELDRGATVVMLGDG
                     RNNRGEPALEELREIGRRSRQLIWLNPESKGVWGTGDSEMLRYRSCCTEVRVVQSIQH
                     LERFADDLLRKATG"
     gene            complement(14957..15922)
                     /locus_tag="PLW13_05900"
     CDS             complement(14957..15922)
                     /locus_tag="PLW13_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQY69940.1"
                     /translation="MSRTELPPRMPTTLMESPAGIRTGFEDAHYICSEQVATTVYLSY
                     HLQKPILVEGPPGVGKTELAKTIAQYLSLPLIRLQCYEGLDESKALYEWKYGKQLLYT
                     QVLKDQLGDVLKGAHGLAESVKRLHEFGDIFYSEEFLEPRPLLKAMRQDGGALLLIDE
                     VDKADQEFESLLLEMLSDYQVSVPEIGTVRAHHTPIVILTSNNTREVSDALKRRCLHL
                     YIPFPDAKLENRIVRSRVPGITDSLRVQMVRFIQELRALDLKKPPAVSESLDWARTLL
                     LLHAHTLDEALVRGTLNVLLKFEDDIQSVNETLLTLLGKARAEAP"
     gene            15998..16807
                     /gene="xth"
                     /locus_tag="PLW13_05905"
     CDS             15998..16807
                     /gene="xth"
                     /locus_tag="PLW13_05905"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY69941.1"
                     /translation="MKLATWNVNSLKVRLPQVIDWLAANRPDALCLQETKTADAGFPF
                     AELEAAGYRAAHNGQKTYNGVAILHRDEATGITRDIPGFADEQKRVIALTLGDTRLVS
                     VYVPNGQEVGSDKYAYKLAWFAAFATWLGEELARWPRLALLGDFNVAPEDRDVHDPGA
                     WRERILCSTPEREAFRGLLRLGLADAFRLFEQPEKSYSWWDYRNMDFRRNRGLRIDHI
                     LLSPALAPDCRSCVIDKAPRKLERPSDHAPVVADIARPGARGTPAAAMSQT"
     gene            complement(16829..17605)
                     /locus_tag="PLW13_05910"
     CDS             complement(16829..17605)
                     /locus_tag="PLW13_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69942.1"
                     /translation="MNGGIMCFHERSDLRWSSYPALLLGVLVSMPALAQVVNNPGYDR
                     PGLGFTPAVLQPGDLMLEQGLPDWSRDDGTSLYTAGTLVRLGVARSLEVQFGTGWNRL
                     EGAGPASDGRSDTSLAAKFAPLTTGNVSWGLLGSVGFTDGAQAFRAEQTQYLLGASIN
                     WQHCTDQSLGLYVEAVDGDADSRLLAVNHGWALSPALGMYVELAAQHRDGVGDGTMGG
                     AGLTWQLTPRVQLDVSARRRLGGHADTWQGGVGIAVYLGD"
     gene            complement(17661..>18401)
                     /locus_tag="PLW13_05915"
     CDS             complement(17661..>18401)
                     /locus_tag="PLW13_05915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY69943.1"
                     /translation="IPRIFNITESAHRIHNPFTPEKLATLGAALRLETGTRVLDLGSG
                     SGEMLCTWARDYGVIGTGIDMSRLFTEQAKLRAEELGVADRVEFIHGDAAGFVSDEKA
                     GVAACLGASWIAGGVIGTIGLLAQSLRPGGIILIGEPYWRQLPPTEDVARGCLAGSVS
                     DFLLLPELLATFGRLGYDVVEMVLADQDSWDRYEAAKWLTMRRWLEANPDDEFAQDVR
                     ARLRSEPERYAAYTREYLGWGVFALMLR"
BASE COUNT         2936 a         6398 c         6250 g         2817 t
ORIGIN      
        1 ttgctgccat ccggatcgat gcccggtcgg cgtttgatca aagtgttgtc aatcaggaga
       61 ctagcaataa attgatgaca aacaacttta agttcggggc ataaggggaa aattcacgtt
      121 cgggggcgaa aggcgctcca tgcggcgagc gtcgcgatga ccgcgaaggc cagcagcgtc
      181 cagccgggta tggcaacgcc gaggaaagcc caatccacct ccgcgcagtt gccgtcaccg
      241 cgcagcagca gcagcagggc atccttcagg ggaaaggcgt cgaacatgta ggccaggtcc
      301 ggcccgcagg cgggcgcctg ccccgggggc aggctctgca gccagagatg gcgcaacgaa
      361 aagccgccgc cgacgaggca ccacagcgtc accagtgaac cgtagacgcg gatgccggcg
      421 cgacgcgggc cgtgcagcgc cgccaccagc gcggtgagcc ccgccagcgt gatgaatatg
      481 cgctgcgtca tgcacagcgg gcacggttcg aggccttcgg catactgcag ccagtaaccg
      541 aagcccatca tcagggcggt ggcgatgaag attcccagga agacctggcg ggacctgatc
      601 caggcgggca acatccgatt ctcgactcca tgaacgcgtg acgggcagtc acgctatgtg
      661 aagcgtcggc gacggtcaat cgccgcgacg ccatcgtgta tcctttgtcg ctcaaccgcc
      721 ggggataacc gcatgtcgat cgcgcgccgc gcctgcctga tgctcctgtg cctgctgcca
      781 ctgatggcgc aggcgaaatc cgcggagaaa gccgaggaag gcggaccgga tttcgagtac
      841 atcgagatgc agcctgcctt cgtggtcaat ttcggcacct ccggtcgcat cggctacctc
      901 aagaccgagg tgtcgctgcg cgtcggcgcg gaggccgtcg ccgcggtcga gctgcacatg
      961 cccgcgctgc gccacgagct catcatgctg ctcagcgggc agagcgccga agcgctggcc
     1021 gcgccggagc agcgcgaggc gctgcggctt gcagcactgg aagccgtgcg cagggtgatc
     1081 gcggcggcgg cggccggcga cgccgcgccc gcgcagtccg gcgtgaagga cctgctgttc
     1141 acctcgttca tcctgcagcg ctgagaggcg ccgcctcaga gcctgtgaat ctttcgacgg
     1201 ccgtcgcgag accgtcgatt tgcgagcgag ccaaggcgcg ctgcgcgaaa acaagggaat
     1261 ttggtgaacc aaatgaccgc cgttttcgcg cagcgcaacg caggcgcagc cgcaaagcgg
     1321 cggtcgcagc aggcgggcga aagattcacc ggctctcagc ccctgaactc gggcgcgccg
     1381 gccatctcga ccagctgcgg atagaacgcg aggaaggact cctcgaactc ccctatccgc
     1441 gaccacgcca cggcggtggc ggcacgcatc gcctgcggct ggcgcagcac ttcccccagg
     1501 cgctccagcg tgccctccag cacctcgacc tcgccatagc ggcagagcag gtcgtgctcg
     1561 acgatgcgac gcgcctggcg cgccgccggt ggcggcaacg ccgccgcgtg ccgctcgagg
     1621 atgatgcaga cgcgcgtgct gaactcgcgc agcgccgggg catggaaacg ctcccagtgc
     1681 cgcgtgagca ggtaatcgaa gaagaggtcg atgaccacgc cggcgaggcg gcgctcgccg
     1741 gggccgaaat gcacccgcag cgcgcgcagg cgctcatggc cgtcggtgaa tgcatcgacg
     1801 cgccggtgca gcctgacgcc gcgctccagc gcctgtggca acgcgccggt gagcggcccc
     1861 ttcacgtaat caccgagcag cgcgcccacg accaggtgct cgcattcacc cgcgaggtgg
     1921 aaatgcgcga ggtggttcac gcgcaggacc tactgcgcgt aatagttcgc gaggtaggtt
     1981 tcgaagggta tcgtgtcggc ccgctcgatg gcgcgctgcg cgtccagcga ctccaccgcc
     2041 aggcgctcga attgcgcgcg cgagacatcg tcgagcgggc gcgccaggaa gtgctcgcgg
     2101 tggagccgcg actgcgccat cgcgaagcgg aagaaggagc cctcgcccgc gcgcagcgcc
     2161 tcgagtaccc gcgccgaggg cgtcagcccg gactgctcga gcttcagacg ctgcgcggcg
     2221 atgctgtcgc ggtattcgac gccgttgcgc gcgcggtcga gcagcacgcc gatcgccgcg
     2281 atgtcttcga gcatgcgcag tccgagttgc cgcagttcca cgctcgcacc gtccgcctgc
     2341 gccaggtgca gccccggctc gcgaccgcgg ttcaccacgg tcagcaggtt ctcgcgcgcg
     2401 agccggcgcg ccggcgcgtc cgaggccggg ctctggtgca gcaggcagaa cagcaggaag
     2461 gcatcgagga agcgcgcagt ggggcgcgcg atgccgatcg gctcgaaagg gtcgaggtcc
     2521 aggcagcgca cctcgatgta ctccacgccc cgttcctgca gcgcgcgacg cggcgactcg
     2581 ccgctgcgcg tgacccgctt cggacggatc gtggcgtaga actcgttctc gatctgcagc
     2641 aggctcgcgc tgagctgccg ccactggtcg ccgtcgcgca cgccgaggcg ctggtagtcg
     2701 ggatgcgcct cggtgatcgc gcgctgcagc gcgccgacgt aatcacccag gctgttgtag
     2761 ggaatatcga tgctgcgttg cgcgttgctc tggtagccga ggtcgcccat gcgcagcgac
     2821 gtgccccagg gcgcgtgcag cgtgccggcg ccgagcggct gcagaccgtg cgctcgcccg
     2881 gccacgaagc tgccacacaa cgccggcgag gcgccgaaca ggtagaccag cagccacgcg
     2941 tggcggtgga aattgcggat cagcgcgaaa tactgctcgg tcttgaaatc ctgcagcgtg
     3001 agcgtgttcg cggtttccgc gcgcagccac tcccagaaat cgtcatcgag cgagaagttg
     3061 tagtgcaggc cggcgatcgt ctgcatcagg cgcccgtaac gatgccccag gccgatgcga
     3121 tagatcgtct tcatgcgcgc gacgttggag tcgccgtagc gcgcgaccgg gatgtcctcg
     3181 tccttttcca gcacgcaggg catgctcgag gtccacaaca gctcgtcgcc gatgcgcgga
     3241 tagacgaagc ggtggatgcg atcgagctcg tccagcgttt gctcgagctc ggtgcctgcg
     3301 tccgtgatga actccagcag cgcctcggaa taatcggtgg tgatcgaggc atgcgtcagc
     3361 gccgaaccga gcgccgcggg atgcggcgtc tgtgcgagcc gtccctgcgg cgtgatgcgc
     3421 aggctttcct tctcgatgcc acggcggata tagcgcaaca gcctgctgtt gtcgcctcga
     3481 tccagggcag cgagccgatg ttcgaattgc gcgttcacat tgtcttcctg gcacacccgc
     3541 gcgcgagccg cgccggggtc gcgagagtgt agtgatgcgg cgcaggcaag tacagcaccg
     3601 ggcgccgacg caggttgttg cgcgtctcgg gcgcgtggta gctttagctc caacccccga
     3661 gaccgaccat gacacggaga ttgaggcgat gccaactccc cagccggcga ttttccgcga
     3721 gaacacgccg catcaatatt tcctcgagtt tcacctgcgc gcacgctggc aacccgagga
     3781 actcaggcgc cttctggggc aggcactcgc cgcgcgcgcg cccgaaacca gcctcgtgct
     3841 ggccttcggc cgcgacgcct gggaacggct cgcgccgcag gaagtgccgg agaacttcat
     3901 caccttccac ccggtgctcg gcaggcagga tcgcattgcg cccggcacgc agggcgacat
     3961 cctggtgtgg ctgcacggcg agcgtcatga cctcaacgtc gccctcgcca tggagatcgg
     4021 tcgcgcgctc gccggcatag caacgctcga gctcgaactg cacggcttca cctacctcga
     4081 ttcgcgcgac atgacgggct tcgtcgacgg caccgagaat ccgcacgacg aggaggcgcg
     4141 cgcggctgcg ctgatccccg cggggcacac cggagccggc ggaagcttcg tactgacgca
     4201 gcagtgggtg cacgacctcg agcgtttcgg cgcgctgggc caggcggagc aggaatcggt
     4261 gatagggcgc accaagcccg acagcatcga gctcgacgac gcgccgccga cggcccacgt
     4321 gcggcgcacc gacgtgcagg tcgacggcca ggcactgaag atctaccgcc gcagcgcgcc
     4381 gttcggcggc ctgcgccagc acgggctgta tttcattgcc ttttcgcgcg agatcatgat
     4441 cttcgcgatg cagctcgatc gtatgttcgg tcgcgccgat gacggcctga tggatcgcct
     4501 gaccgaattc tccacccccg tctcgggcgc ctactggttc gcgcctccgg tggaatcgct
     4561 gaccgcgctc ttcggcgacc atgccggctg agccgtgcgt cgcacccacc gggcgcgctg
     4621 aagacggatc gagcgcgcta cccgaggaaa cgacccgatg cgacgcctga cgcgcaacgc
     4681 gatgctgatc gcactggccg gcgccacggc attcgccggc tggcaactgc tgcgcgggcg
     4741 cggggccgcc gtcgaggcct tcgagccgcc ggcgccagcg gtgcgcgcgg ccacggtcga
     4801 acggcgcgag atcgccgacc gcgtcgaggc gctgggcacc acgtcatcgt gggaatcgat
     4861 cgagttgcgc cccaccgtca ccgagttcgt cagtgcgatc catttcaccg atggccaggc
     4921 ggtgacgcgc ggcgacgcgc tggtgacgct ggcgcagcag gaggagagcg cgcagctcga
     4981 cgaggcccgc gcctacctcg acgagcagct gcgcgaggtg cggcgcatcg agggactggt
     5041 ggcgcgcaaa tcgctgccgc agagccagct cgacgagcgc cgcacgctgc gcgacatcgc
     5101 gctcgcgcgc gtgtccgcgg cgcaggcggc gctggccgac cgtaccatcc gcgcgccgtt
     5161 cacggggctg ctcgggctgc gcaacgtgag ccccggcgcg ctggtgacgc cggagacggt
     5221 gatcaccacg ctcgatgacg tcgccacgct gcgtctcgat ttcacggtgc cctccgcgct
     5281 gctgtcacgg ctgcgcacgg gagaggcggt cacggccgcg agccccgcct tcgagggccg
     5341 cgagttcagc gccacggtga cgggcatcga ttcacgcgtg aacccggtag accgctcgct
     5401 cacggtgcgc gcgcgcatcg acaacgccga cctggcgctc agacccggca tgctgctcac
     5461 gctggcgctc gagcaccacc cgcgcaccgc gctggtggtg cccgaggaag cgctggtgca
     5521 ctaccagcgc tcgcacttcg tgctggtcgt cgacggcgcc gacggcaacc gcctggtgcg
     5581 ccgcgaagtc acgctgggca cgcgcattcc gggcagcgcg gagatcctct ccggcctcga
     5641 ggagggcgag ctcgtcgtgg tggaagggct caccaccgcg cgtcccgggc agcaggtccg
     5701 tatcctcgcg ccgcagggtg tggaatcgcc gccatgatgc tctcggacct gtcggtgcgc
     5761 cgccccgtgc tggcggcggt gctcagcctg ctgctgatcg ccttcggcgc ggtggccttc
     5821 gacctgctgc cgctgcgcga gtaccccgac atcgatccgc cggtcgtcac catcgagacc
     5881 acctaccgcg gggcgtcggc ggccgtggtg gaaacgcgca tcacgcagcc gctcgaggaa
     5941 cgcatcgccg gcatcgcggg actggaagcc atcagctcgg gcagcgagga cggcgtgtcg
     6001 ttgatacagc tcgagttctc gctcgaacgc gacatcgacg atgcggcgaa cgacgtgcgc
     6061 gatcgcgtcg gcggcgcact ggaccagctg cccgcggaag ccgatgcgcc ggacatccgc
     6121 aaggaggacg cgtcgggacg cgtgatgctg tggctcaacc tggtcagcga ccgcatggac
     6181 cggctggagc tcaccgatta cgccaaccgc ttcatcgccg accgcttcgc ggtgatcgac
     6241 ggcgtggccc gcgtgcaggt gagcggcgga ctcgattacg cgatgcgcat ctggctcgac
     6301 cgcgacgcgc tcgcggcacg cgcgctgacg gtagccgatg tcgaggcggc gctggcgcgc
     6361 gagaacctgg agctgcccgc gggcgccatc cgttcggagc aacgcgactt cgtggtgcgc
     6421 atggagcgca gctatcacac cgcggaagac ttccggcggc tggtgctggg caagggcggc
     6481 gacggacacc tggtgcggct cggcgaggtc gcgacggtgg aactgggcgc cgcggagaac
     6541 cgcaggcagt tccgcggcaa cggcgtgccg atggtcggca tcggcatcgt caagcagagc
     6601 aatgccaata cgctggcggt ggcgcgcgcg gtgcgtgccg agcgtgaccg caccaacctc
     6661 tcgctgcccg agggaatgga gctcaagcag agctacgact cctcggtgtt catcgagagc
     6721 gccattgccg aggtctattt cacgctgttc atcgcctgcg gactggtgat ggtggtgatc
     6781 tggctgttcc ttggcgacac gcgtgccatg ctgatcccgg cagcgacact gcccgtgtcg
     6841 ctgatcgcga ccttcatcgt cctctacttc ttcggctatt cgatcaacct gctgacgctg
     6901 ctcgcgctgg tgctcgcgat cgggctggtg gtggacgacg ccatcgtggt gctggagaac
     6961 atccaccgcc gcatgcgcgc cggcgagacg ccgctggtcg ccgcgtggcg cggcacgcgc
     7021 caggtcggtt tcgcggtggt cgcgaccacg ctggtgctgg tgtcggtgtt cgtcccgatc
     7081 accttcctgc aggatgacat cgggcggctg ttcggggaat tcgccgtagc gatggccgcg
     7141 gcagtgctgt tctcggcgct ggtggcactc accctctcgc cggcgatgtg ctcgatcatg
     7201 ctgcgcccgg actccgcgga gcacggcgcg ggccgcgcgt tcacgcgcgc tcaggccgcg
     7261 tatcgccggt cgctagccgg cgtgctgcac catccgtgga tcgcgctggc ggtgtttgcg
     7321 gcggtcctcg ccggtgcctg gctgctgatg cgggccgttc cctccgagtt cgcgccccgc
     7381 gaggaccgcg gcaccttttt catgttcatc accgcgccgg agggttcgtc cttcgactac
     7441 acgatgcgcc atgtcgacga tatcgagcgg cgcatcatgc cgatggtcga gcggggcgaa
     7501 atcgagcgca tgctgctgcg cgcgcccgcc ggctccacgc gcaccgagac ttacagcgcg
     7561 gcgacgggca tattcaacct gccgcactgg gacaccgggc gccccccggc gtgggagacg
     7621 atcgcggagg tgagccggcg catcgccgac gtgcccgggg tgcaggcctt cccgaccatg
     7681 ccccagggcc tcggcggacg attcgagaag ccgctgcagt tcgttatcgg cggcggcacc
     7741 ttcgaggagc tcgcgcaatg gcgcgacatc ctgctcgagc gcattgcgca gaaccccggc
     7801 ctgaccgcgg tcgaccacga ctacaaggag accaagccgc agctgcgcat cgaggtggac
     7861 cgcgcgcgcg ccggcgatct cggtgtcagc gtcgagaatg tgggccgcac gctcgagacg
     7921 ctgctcggtt cccgtcgcgt caccaagctg ctgatgaacg gcgaggagta cgacgtgatt
     7981 ctcgaggcca tcgaggacac caagcgcagc agggcagata tctcgaacat ctacgtacgc
     8041 tcggcgacga gcggcgcgct ggtgccactg gcgagcctgg tccgtaccga ggagtctgcc
     8101 gatgcgggcg ccctgaaccg ctacaaccgc acgcgcgcca tcacgatcga ggcgaacctc
     8161 gccgatggct acaccctcgg cgaggccatc gacttcatgg agaacgccgc gcgcgagctg
     8221 ctgccgccgc gcgccaccac ggacctcaag ggcgaatcgc gcgacctgcg cgaatcgggg
     8281 cgttcgatct acctgatgtt cctgctctcg ctgctggtgg tctacctggt gctgtcggcg
     8341 cagttcgaga gtttcctgca cccgctgatc atcatgctga cggtgccgct ggcggtggtg
     8401 ggcgcactgc tgggcctgtg gctcacggga cagacgctca acatctacag ccagatcgcg
     8461 atcatcatgc tggtggggct ggcggcgaag aacgggatcc tgatcgtgga attcgcgaac
     8521 cagctgcgcg ccgagggcct cgagcgcgaa gaggcgctgc tggaggcctc ctcgctgcgg
     8581 ctgcgcccga tcatcatgac ctccatcacc acgatcatgg gtgccgtgcc gctggtactg
     8641 tcgggcggcg cgggtgccga gacgcgcttc gtgatcggcg tggtcgtgat cgccggcgtg
     8701 ctggtctcga cgctgctcag cctgatcgtc gtgcccctga cctaccactg gctgaccgcg
     8761 cgcgcgccgc tgccgggcgc caccggccag cgcctggagc gcgagctggc cgcgcatggc
     8821 gcgagcgaag cgcgcgcagg cagcggcatg tgacgacggg gtgcagctga cgctgcgttt
     8881 ccctggcgag atcggctcga gcgcggcggc tcgctcagag cccgcggaag ccgaaatgct
     8941 ccggggcaaa cgcccggtag gtcgcatcca ccacgtccgg ttcgaatccg aggcacgaca
     9001 ccacgtgcgt gtggatgccg ccgcgcgcgt aatcgagcac gcgctgctgg cactgctcgc
     9061 gcgtgccgat gatcgcgatc tcgtcgacca gctcgtcgct gatggccgcc gcggtgcgct
     9121 cgcggtcgcc cgcggcccag ccttcgcgga tctccctggc ggcgtgctcg tacccggccc
     9181 aggcaaggaa ctcgttgtac accggcgtgg cgtagtacga ggcgaacacc atgcggaaca
     9241 gcttgcgcgt cgccgcggga tcaccggtga ccgcgatctg gtgacggcag acgatctcga
     9301 cttcggccgg atccttgccg gcgcgccgcg caccgcgcgc cacgtgctcg acgatgcgcg
     9361 gcagcgcccg gcgcggaaac aggttcagga tcacgccgtc gccgagctcc gccgccagct
     9421 cgatcatgcg cgggcgcagc gccgcgagat ggatcggcag ccctcccgcc accgcctcct
     9481 ggcggtagcc gtggctcttc agggtctcgc cgctgaagcg cgtcttctcg ccggccagca
     9541 tctggcgcag cagctgcacc gtttcgcgca tgcgcgtgag cggctgcgcc agttcaagac
     9601 cgtgccagcc ttcgatgatc gcgtggctcg aagtgcccag gcccagcacg aaacgtcccg
     9661 gcgccacctg cgcgatggtg gccgcggtgc tcgccagcac cgcgggggtg cgcgtgtacg
     9721 cgggcaccac cgcgatgccg atgcgcgccc gcgcggtgtt ctgcgcgatc agcgccgcca
     9781 gcgtcagcgc atccatgccg ccggcgtcgg cgagccacag gtcgtcgtag cccgcgctct
     9841 cggcacggcg cgcgagcgcc agggtgtcgg cgacgttgcc gctcgatccg ggaagtgtga
     9901 tggccgtgcg aaaacggggc ggcatggtgg ctctctcctc ttcatggcgt tgtggcgcga
     9961 gtctagccga cacggggccg cgctcacagc ggcgcctcgg cgcgccctgc cggtgaccgc
    10021 cggcttctct ggccgcgact aacgcgctac tgtttccgcg cgttcataaa aaagcgaggg
    10081 cggagggatt gctcatgtgc tccactccag cccgccagag ccgcggagat ttccgatgaa
    10141 aacctacgct tacccgctgc agttgctcgc cgaccacgtt gcgcgccatc cggagcggcc
    10201 ctacctgcac cagccggtgg atcgcgtctg gcgcaccttc acgtggcgcg acgtcgacgc
    10261 gcaggcgcgg cgcctcgcca acggcctgct ggcgcagggc tacacggcgg gcgatcgcat
    10321 cgcgatcctg tccaagaact gcgccgaatg gttcatcgcg gatttcgcga tcatgatggc
    10381 cggactggtc agcgtgccga tctaccccac cgcctcggcc gcgaccatcc gctacgtgct
    10441 cgaacacagc gaggcgcgcg cgatcttcct cggcaagctc gacgccacgg cgcccgccga
    10501 cgaggcgatc gggcccgagg tgctgcgcat cgcgctgccc taccccacgg cgccggcggc
    10561 cgcgcactgg aacgactggc tggcgcgcca ccagccgctg gccgagaccg cgcagcagcg
    10621 tcccgacgac atcttcacgc tggtctacac ctcgggcagc accggggtgc ccaagggcgt
    10681 ggtgataagc caccgcaaca tggcggcctc ggcgcagagc acgcgcgaca acgtgaaggc
    10741 gcgtcccggc gaccgcctgc tgtcgtacct gccgctcgcg cacatcgccg agcgcgccgt
    10801 ggtggagcag ctctcgctcg ggctcgacgt cgaggtctac ttcgtcgagt cgctggacac
    10861 cttcgccgag gacctgcgcc acgcgcggcc cacggcgttc atgtcggtgc cgcggctgtg
    10921 gacgcgcttc cagtcgcaga tcctgcgccg catgcccgat gcgcgcctgc agcgcctgct
    10981 cgccataccg ctggtcggac gggtggtggc acggcgcatc cgcaaggcca tgggcctcga
    11041 tagcgcgcgc ctgttcggct ccgggaccgc gcctatttcg ccggccatgc tggcctggtt
    11101 cgcgcgcctc ggcatcccga tcagcgaggg ctggggcatg accgagacca cggggctgtc
    11161 gtgcagcaac tacccgtacc gccccgactg ggtgggcacc atcggcaagc cgctcgactg
    11221 cgtggagatg acgctctcgc cggcaggcga gatcctggtc cgcggcgatg cggtgttccg
    11281 cgagtactac cgcaaccccg aagccaccgc ggcgaccttc cgcgacggct ggctgctgac
    11341 cggcgatatc ggcgagcagc gccccgacgg ggcgttccgg atcatcggcc gggtcaagga
    11401 gcagttcaag accgccaagg gcaaatacgt ggcaccggtg ccgatagaga gcctgctcgc
    11461 cgcgaacccc gtgatcgacc aggtctgcgt gatgggcagc ggccgcaagc agccggtggc
    11521 cctggcggtg ctcgccgagg gcgagccgcg cagcgaggcg gagctggcgc gcgagctcga
    11581 aagcctgctc gagcaggtca acggccagct cgagagccac gaggcgctcg atcacatcgt
    11641 ggtctgcgcc gagccctgga gcatcgagaa cgagttgctg acgccgaccc tgaagctcaa
    11701 gcgcgagcgc atcgaggccc gctacggccc gctgctcgag agcaccccgc tgcaccagcc
    11761 cgtggtgtgg gaacagttgc gctgagacac ccccgcctcc cggggctggt caacggaatc
    11821 gcggcccgcg cgttttaagc gtcaactcca ccgtgggtcc gtcggatgaa agccctgttg
    11881 cgttgcctgt tgtcgctgct gttgctgacg tcgtatggct gcgccgcgca accacgcgag
    11941 gaggcgccgc tgttctccca ggcagagctc gaccagatgc tggcgcccat cgccctgtat
    12001 ccggaccagt tgctgtcgca actcctgatg gccgccacct acccgctgga agtggtgcag
    12061 gcggcgcgat ggtcgcgcga gcaccccggc ctgaaggggc cggaggcagt gcgcgcggtc
    12121 gaagacaggg cgtgggatcc cagcgtcaag tcgctggtcg ccttccccga cctgctggca
    12181 cagatggacg ccgacctgga ctggaccgag cgcctgggcg atgccttcct ggcgcagcag
    12241 cagcaggtga tggacaccgt gcagtcgctg cgccagcggg cctacgatgc cggcaacctg
    12301 cgttcgggcg agcaggtgcg caccgtgcgc gagcgcgagc tcatctacgt gcgtccggcg
    12361 tatccggacg tgatctacgt gccctactac gatcctttca cgatctacgg ctcgtggtgg
    12421 tggccggatt atcgtcccgt gtactgggcg ccgtggccga gctatggcgt gtcgctcggc
    12481 tacgccagcc acggcgggtt ctactggggc gcgggataca acctcggcgt cggcttcttc
    12541 ttcagcgact gggactggca ccgccaccac gtccgcgtgg tggattaccg ccccttctac
    12601 tatcgccggc caccttacgc gcagcacatc tgggtgcacg actacagcca ccgccgcggg
    12661 gtgccgtacc gccaccgcgc cgtggagcac cgctacgtgc ccgtggacag ccgtccggtg
    12721 cgccggcacg actacggcga ttaccgcgac agcgaccgtg actaccgcta ccgggatggc
    12781 gagcgcaggg atgatcgccg cagccaaccc ggacaggagc ccggtcgcga gttcaaccgg
    12841 gcgccggagc gcaaccgcga tcgcagtttc aacacggacc gcgcgacgac tgggccagcg
    12901 acgatcgaac cgcgcacggt cgcgcccggc gaaaacggcg tgcgtcgcga taacggcatg
    12961 cgtcgcgaaa cgatgccgca accggccgat cgcgccgcga cgccgccgga gcgctatcgc
    13021 gaggcccggg gtgacgcgag cagccggccg gacaggatcc gcgaacctcg ggatagcgcg
    13081 accagccggc cagagcggat ccgcgagcct cgggataccg cgaccgcccg gccagagagt
    13141 atccgcgagc cctggcccgg cgagccgggc atgcagcggc gcaccgcgac gccgcgcgcg
    13201 agcgaagcgc cgctccagcg cccggcgacg ccgcgcgagg tgcaacgcta cccggcgccg
    13261 cgcaacgtgc agcgcgaaat gcccgagcgc gtgcagcccg aagcgctacc gcgtgcacgc
    13321 agttacccgc aggccgcacc ggcccgtgaa ccgcgctccg atggcggggg cttcgtggca
    13381 ccggatcgcg gcacccgcga acccctgcga tccgccccgc ccaagggccg cagcgacatg
    13441 tcgcaggagc gcggagcaca gcgcagcgac tccgccggcg gctttccacg cggggatcgt
    13501 ggtgatcacg gggatcgtgg aactcgcgcc ggtcgcggct ttcgcgacca accctgaggg
    13561 cttcagcccg tcgccttgcg cagcaggtcg tcggcaaagc gctccaggtg ctggatcgac
    13621 tgcaccacgc gcacctcggt gcagcagctg cggtagcgca gcatctcgga atccccggtg
    13681 ccccagacac ccttcgactc ggggttgagc cagatcagct ggcggctgcg ccgcccgatc
    13741 tcgcgcagtt cctccagcgc cggctcaccg cggttgttgc gcccgtcacc gagcatcacc
    13801 accgtggcac cgcgatcgag ctcgcgcccg gtggcggcga gcaggccgcg gatcgccgcc
    13861 gcgtaatcgg tcgaaccgcc accgtactgg cgctgcgcca attccaccgc gacctcgggc
    13921 gggttgtcct cgaacaacgg cgtgacctcg gccaactgcg acgagaacac gaagctgcgc
    13981 acgcgcggca gcacgtcact caggctgtag aggaacagca acaggaagcg cgcataggcg
    14041 ctcaccgagc cgctgatgtc gcagaccgcg atcagcgccg gacggtcctt tcgcacgctc
    14101 ttccaggccg gctcgaagag caacccgtcg tgcgccgcgc ccttgcgcag tgtcttcggc
    14161 acgtcgagca gaccgcgcct ggcggtgcgc cggcgccgcg cgtagcgcgc cgacagccgg
    14221 cgcgcgatgc gccgcaccag gcggttgatc tgctgcaggt cgcgccgctc gagctgcgac
    14281 agccgcgccg aacgcagcac cgactcgcgc agctcgcgcg cgttggcgct ggcgaacagc
    14341 aggtactggt cctcgacgta ttcgcgcacg cgatcgcgca gcatctcggc gccgcgtttc
    14401 agcgcgatgc ccgcggcgtg acccggctcg gcttcgggcg gcgcctgctc gaggccgagg
    14461 atctcggcct gcagctgcgg ccagcccagc tgttccagca cgcgccgcga atacaacccg
    14521 cgctgcgtga acaccttcat gtcgccgatg cccgccgcgc gcccggcggc atcgatcgcg
    14581 atccgcagcc cggccgcgtc gcccgcgagc agcagctgcg ccagctccga cagctcgccg
    14641 gccatcgcgc cctgcgcggt cgaggcgccg ggcgccgcca cgtcggcccg cggcggtatc
    14701 acggcagcca gtgcgtcctg cgcctgcccc gcctccggcg gccggcgcca cgcgaagaag
    14761 cgctcgaagc actcgtcgaa cagcgtcttg tcgtcgatcg tcttggccag cgtcgcggcc
    14821 agcgccaggc gcacgcgctc gcgatcggcg tagcccacca gttccagcgc gcgcgccgcg
    14881 tccatggttt ccgccggcga cacgcggatc tcggcgttgc gcagcacgcg cacgaaatcc
    14941 gtcagcgtgc tttccatcag ggcgcttcgg cgcgcgcctt gccgagcagc gtcagcaggg
    15001 tctcgttgac ggactggatg tcgtcctcga acttcagcag cacgttcagc gtgccgcgca
    15061 ccagcgcctc atccagcgtg tgcgcgtgca gcagcagcag ggtacgcgcc cagtcgagcg
    15121 actcgctgac cgcgggcggc ttcttcaggt cgagcgcgcg caattcctgg atgaagcgca
    15181 ccatctgcac gcgcaggctg tccgtgatcc cgggcacgcg gctgcgcacg atgcggttct
    15241 cgagcttcgc gtcgggaaac gggatgtaga ggtgcagaca gcggcgcttg agcgcatcgc
    15301 tcacctcgcg cgtgttgttg ctggtgagga tcacgatcgg cgtgtggtgc gcgcgcaccg
    15361 tgccgatctc cggcaccgag acctggtaat ccgagagcat ctccagcagc agcgactcga
    15421 attcctggtc agccttgtcg acctcgtcga tcagcagcag cgcgccgccg tcctggcgca
    15481 ttgccttcag cagcgggcgc ggctcgagga attcctcgct gtagaagatg tcgccgaact
    15541 cgtgcaagcg cttcacggac tccgcgagcc cgtgcgcacc cttcagcacg tcgccgagct
    15601 ggtccttcag cacctgcgtg tacagcagct gcttgccgta cttccattcg tagagcgcct
    15661 tggactcgtc gagcccctcg tagcactgca gccggatcag cggcagcgag aggtactgcg
    15721 cgatggtctt cgccagctcg gtcttgccca cgccgggcgg accctcgacc aggatcggct
    15781 tctgcaggtg gtacgagagg tacacggtcg tcgcgacctg ctcgctgcag atgtaatggg
    15841 catcctcgaa acccgtgcgg atcccggcgg gggattccat cagcgtggtc ggcatgcgcg
    15901 gcggcagttc ggtgcggctc atcgggttcc cgtgtgctgg cgtggtggcg cgagtatacg
    15961 cgacggcccc cgcccgggcc tcgggtatga tgcggccatg aaactcgcaa cgtggaacgt
    16021 caattcgctg aaggtgcggc tgccgcaggt gatcgactgg ctggcggcca accggcccga
    16081 tgcactgtgc ctgcaggaga ccaagaccgc ggatgccggg tttccgttcg ccgaactgga
    16141 ggccgccggc taccgcgccg cgcacaacgg ccagaagacg tataacggtg tcgcgatcct
    16201 gcatcgcgac gaagccaccg gcatcacgcg cgacattccc ggcttcgccg acgaacagaa
    16261 gcgcgtgatc gcgcttacgc tcggcgacac gcggctcgtc tcggtctacg tgccgaacgg
    16321 gcaggaggtc ggcagcgaca agtatgccta caagctggcg tggttcgccg ccttcgcgac
    16381 gtggctcggc gaggaactgg cgcgctggcc gcgcctcgcg ctgctggggg atttcaacgt
    16441 cgcccccgag gaccgcgacg tgcacgaccc cggggcctgg cgcgagcgca tcctgtgcag
    16501 cacgccggag cgcgaggcct tccgcggact gctcaggctg gggttagcgg acgcgttccg
    16561 gctgttcgag cagccggaaa aaagctattc gtggtgggac taccgcaaca tggatttccg
    16621 ccgcaaccgc gggctgcgca tcgaccacat cctgctctcg cctgcgctcg cgcccgactg
    16681 ccgctcctgc gtcatcgaca aggcgccgcg caagctcgag cggccctcgg atcacgcgcc
    16741 ggtggtggcg gacatcgcgc gtcctggagc acgcggtacg cccgctgcag cgatgagtca
    16801 gacctaaggc ccgcgcagca aattttcttc agtcaccgag gtaaacggca atgccgacgc
    16861 ctccctgcca ggtatcggca tggccaccca gacgccgacg ggcactgacg tccagttgca
    16921 cccgcggtgt cagctgccag gtcagaccag cgccgcccat ggtgccgtca ccgacgccat
    16981 cgcggtgttg ggccgccagc tccacgtaca tgcccagtgc cggggaaagc gcccagccgt
    17041 ggttgaccgc cagcaaccgg ctatcggcgt cgccgtccac cgcttcaacg taaaggccca
    17101 gcgactgatc cgtgcagtgc tgccagttga tgctcgcccc cagcaggtat tgcgtctgtt
    17161 cagcgcgaaa agcctgcgct ccatcggtga acccgacgct gccgagcaat ccccaactga
    17221 cgttgcccgt ggtcaatggc gcgaatttcg ccgccagcga ggtatcggat cggccatcgc
    17281 tggcgggacc ggcgccttcg agtcgattcc agcctgtgcc gaattgcact tcgagcgagc
    17341 gggcaacgcc cagacgaaca agggtgcccg ccgtgtacag cgaggtcccg tcgtcgcggc
    17401 tccagtcggg cagcccctgt tccagcatga gatcaccggg ttgaagtacc gcgggcgtga
    17461 accccaggcc gggacggtca tagcccggat tgttcacaac ctgcgccagc gccggcatgc
    17521 tcacaaggac tccgagaagc agggccgggt aagaactcca gcgcagatcg ctcctttcgt
    17581 gaaaacacat gattccgcca ttcatatgca tcgattcata cggcctgtcg cctgaaccag
    17641 gccgcgcggc attcctctca tcaccgcagc atcagtgcga acacacccca gcccaggtat
    17701 tcacgcgtgt atgcggcgta gcgctcgggc tccgagcgca gcctggctcg aacatcttgc
    17761 gcgaactcgt cgtcgggatt ggcttcaagc catcggcgca tggtgagcca cttggccgcc
    17821 tcgtatctgt cccagctgtc ctggtctgcc agaaccattt ccacgacgtc gtagccgagg
    17881 cggccaaaag ttgcgagcag ttccgggagc aggagaaagt cggaaactga gccggcaaga
    17941 caccccctgg caacatcctc cgtcggcggc agctgccgcc agtacggctc gccgatgaga
    18001 atgatccctc cgggacggag gctctgcgcc aggagcccga tggtgccgat gacgcccccg
    18061 gcgatccaac tggcgccgag gcaagctgcc acgccggcct tctcgtcaga gacgaagccg
    18121 gcagcatcac catggatgaa ctccactcga tcggcgacac caagttcctc tgcgcggagt
    18181 ttcgcctgct cggtgaacaa gcggctcatg tcgatgccgg tgccgatcac tccgtaatcg
    18241 cgtgcccagg tgcacagcat ctcgcccgaa ccgctgccga gatcgagcac tcgggttccc
    18301 gtttccagac gcagcgccgc gccgagcgtg gcgagctttt ccggtgtgaa cgggttgtgg
    18361 atgcggtgag cactttcggt gatgttgaag atccgtggaa t
//
LOCUS       DAORUQ010000045        18228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2710_length_18228_cov_7.013041, whole genome shotgun sequence.
ACCESSION   DAORUQ010000045 DAORUQ010000000
VERSION     DAORUQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_18228_cov_7.013041"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLW13_05920"
     CDS             <1..420
                     /locus_tag="PLW13_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HQY69944.1"
                     /translation="RKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVA
                     AGLAERCEIQVSYAIGVAEPTSISVNTFGTGKLSDAAITDLIRQHFDLRPRGIIEMLE
                     LRRPIYRATAAYGHFGREEPDFSWERTDKAAALAKAL"
     gene            623..2008
                     /gene="ahcY"
                     /locus_tag="PLW13_05925"
     CDS             623..2008
                     /gene="ahcY"
                     /locus_tag="PLW13_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003160905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQY69945.1"
                     /translation="MTTAQKAFTDYKVADISLAAWGRREIAIAESEMPALMAIRARYR
                     DSQPLKGAKILGCLHMTIQTAVLIETLAELGAELRWSSCNIFSTQDHAAAAIAASGVA
                     VYAWKGETEEEYEWCIEQTILKDGKPWPVNMILDDGGDLTGILHERYPQLLDKVHGIT
                     EETTTGVLRLQEMLKHGTLKVPAMNVNDSVTKSKNDNKYGCRHSLSDAIKRGTDHLLA
                     GKKALVIGYGDVGKGSAQSLRQEGMIVRISEIDPICAMQACMDGYEVVSPYLDGRNDG
                     SAEGIDRQLLAQTDLVVTATGNMDVCDHHMLAALKCGAVVCNIGHFDHEIDTAFMRRN
                     WEWEEVKPQVHMIFRNRATNDHLILLSEGRLVNLGNATGHPSRIMDGSFANQVLAQIY
                     LFERRFADLEPEMKPAALSIEVLPKQLDEEVARHMVAGFGGVLTRLTPQQADYIKVPV
                     EGPFKNEQYRY"
     gene            2018..2863
                     /locus_tag="PLW13_05930"
     CDS             2018..2863
                     /locus_tag="PLW13_05930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQY69946.1"
                     /translation="MGNSSRALRLSCEYFPPKTEDGKHKLLAAHDKLQALKPEFFSVT
                     YGAGGSTRSNTRDMVLELKRRGSCVAPHLSFGGDDESLVLELLQVYRDNGIRRLVALR
                     GDMPSGMGGMSQMVYAAELVGFVRRHTGAHFTIEVAAYPEMHPQAPDMIADVRFLKRK
                     FDAGADSALTQFFYNADAYFRFIDACRAEGITQPVYPGIMPILNYQNLARFARNCGAE
                     IPRWLHYRLESLESDAAGTREFAEEFVASLCRRLLEGGAPGLHFYTMNQAEPTLAIAR
                     GAGLL"
     gene            2866..3594
                     /locus_tag="PLW13_05935"
     CDS             2866..3594
                     /locus_tag="PLW13_05935"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQY69947.1"
                     /translation="MRAPRLFVETALATGAAIELKDEALHYARNVLRLDVGAPLLLFN
                     GEGAEFEAAVTAAGKRSLTLAVGIDTGAAREATLAVHLGIGLARGERMDWVVQKCTEL
                     GVASITPLLLARCNAKFDQGRGDNRRRHWRQVAISACEQCGRNRLPLIGEPVTLDHWL
                     ATRTEAPGFVLHTADSHALARGDAPAAVRLLVGPEGGLDSNEFAQARSAGFTPWSLGP
                     RVLRTETAPVAALAILQYLWGDLA"
     gene            complement(3634..4092)
                     /locus_tag="PLW13_05940"
     CDS             complement(3634..4092)
                     /locus_tag="PLW13_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQY69948.1"
                     /translation="MNAVPGPRDLSALLVPVTGQLLVVPGAVVAEIIKRRDLQRPPAA
                     PDWLLGTLLWHHEKLPVLSFEALNGHAGPDPGHGSRIVILSTLSDAAPARNYAILAQG
                     VPHLLLLTAADVQAVDAPAPGPAERMKVRVHGQDAAIPDFDFIEQHLAAP"
     gene            complement(4089..10319)
                     /locus_tag="PLW13_05945"
     CDS             complement(4089..10319)
                     /locus_tag="PLW13_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQY69949.1"
                     /translation="MQQDFVALQWVGGEIDEIAGQFGKAVLGFADNVSDQTRLRLGLT
                     QAHQLHATLRLLGVPSAEQLAREIEDTVQAMLHGRIEPSGANLQLLLAAGMQLPAYLH
                     RVAAERRESPLDVLGLVNELRAARTEAPLAAPPLADTIDTAELAQGSGVSAAQADDEE
                     VQLLRLARQRFQAELLGVLRHEATGEHLRNMGKLFALLAQRLRPGGHRLFWQACAALA
                     EALDQGGVALDEAAIAVLRELDGELRAGVALEHGLYRRTPDAGLYRRLLELIAQSRPV
                     SALLAEIQQRHRLTPDAAVAAGPATLDDLGATGAAVAVLAAELSAVLARLEAAEGDAF
                     SISRVLREVEPDLRRVGQLLDGLGYQEEHEILQPQLERIQSGLVGGADLDDRLTEISA
                     ALLDVDQRLASRAVQARGEQDELAESRRHPLQRALAAVYREVRAGLEEVKRTVGDYVT
                     SAADTTLLAGAPERLHALAGALRVAGLDGAAQVLGECRDHLGAVLAGSRAAPDSQAVE
                     ALADALGSVEYFLERLAIDQVPAELILGRARESLARLAALPQVAGLVEAPGAPQPEVA
                     IAATAVPLAVAQEAGAPAPAEPEQPLAPAALVAAEHAVAVADPEIVEIFTEEAAEVLE
                     TIAAQLPPWRSAPGGGAPLAELRRAFHTLKGSGRMVGAAHIGELSWSVEQLLNRVIER
                     TLEPSSAVVEAVERAHQLLPALVRAFAGGPSVDESTVRTLQSAVQAVTRGEAPQWAPT
                     AAEAPHEQVAEELLPGTLAPEEWLPEAEAVPELAEELVAEPESLAGQAEGQAPDPEIA
                     AIFTEEAAEVLERIATQLPLWRAAPLAGAPLEELRRAFHTLKGSGRMVGAELVGDLSW
                     SVEHLLNRVTERALEPTPAVMEAVERAQQQLPALVQAFAGGAAVDAAAVRALQETVES
                     LTTREPSQWALTPVEAPQEPLAEELLPVESAEQAWLPGAELQESVAEELLPQVAAPEE
                     WLPEAGAEPEFVEEMSPGESEPVVEAAADEEAARLHAIFAEELAEHLRALRDYLAGAE
                     RGTVTQLLLRTLHTLRGSALAAGNEALGRVLDAVDELVRAALEAERALQAPELDQLQQ
                     LCTLLDAVLAAGARDSAQLSADAAALREQVLACARTEPARVPAAALDARLARFLEEAL
                     EKLFHATTLLEAWRADRDAEVSRRTLLEELQVIDQRAEQLNIAGMLELSRPLLAALRP
                     AAHGVTRPAAELFELARRGLEAFLEVMDRLAAGQLPEAPQAIIAQLHEAAAAQAAPES
                     APGPVPAPQRAGGEAEEELLRARVGRDVQHAEHELLGIFMEEAEELMGAIDESIEAWR
                     HDRGSRAPFDDLQRHLHTLKGGARMAGLRYLGELSHNFETFLINQAVRFGAFDDAFFA
                     RVADFHEQLLRTMDVVKERPVAAPAVLAAVAEAVPAAPEQAEPVAPAERVASEVAPAA
                     PLAAVPPGVAPRRPAPAVAVPPGATKPRDEVVRVSAQLLEDLVNLAAETSISRGRVEE
                     QVSELGQLFEDMQGAIERLQSQVRRLDIATEAQVLFRRERAENAAVDGFDPLEMDRYS
                     QLQQLSRSLVESASDLLDIKRTLAEKTRDLETVLIQQSRINSQLQEGLMSSRMVPFSS
                     ILPRLKRVVRQVAGELHKQVELELGSIAGELDRGILERVVAPLEHMLRNAVDHGIETA
                     ELRRERGKPATGHIGIEVAREGGDVLIVVADDGGGVDLAAVRAKAVERGLMEPDAQLG
                     DHEILQFILASGFSTAAAVTQISGRGVGMDVVSSEIRQMGGSLEIHSEPGRGTRFVVR
                     LPFTVSVSRALLVTVGPEIHALPLNSVEGVVRMRADELRHYAGPDAAPFEYAGQSYQV
                     RHLGAVLYPDEQPDPGSLSDTVPVVLVRGGGQALAVQVDRLVGAREIAVKSLGPQFGA
                     VPGLSGATVLGDGSVVVILDIPAMLRADAAHGATGYEHAPRGAQEHKAERPPLVMVVD
                     DSVTVRKVTTRFLEREGMQVITAKDGADAMAKLQEQVPDVMLLDIEMPHMDGFEVVSK
                     VRLSEQLRRIPIIMITSRTGDKHRERALSLGANMYLGKPYQESVLLEHIHALLARQGA
                     SS"
     gene            complement(10337..12385)
                     /locus_tag="PLW13_05950"
     CDS             complement(10337..12385)
                     /locus_tag="PLW13_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQY69950.1"
                     /translation="MNAKQNVPAASQPNVRWILVWGGVLVVAVLAFVWNLFVIMRDTN
                     RDAGYRELVGNLRVLSQQIEANAREAVDGDAAAFTNLESARADFDRTLDDLANGTDAL
                     PSPREALAEELALIGETWSAVDPAASTIVARKDSIEFLSKTSATLAGSIEDVQKRTAR
                     IADVLAGSGATIRQVALAERQSWLAERISRNLDRMLEGGSGATEAAEQFNADTESFAD
                     VLDGMLEGSAAMGIPALVNAGALADAREVKELFRVIGGGKQRILDSAPDLKQARQAAE
                     AIVQSAPKLLNATSVTSDRINDLEGERKLNMGSALAFGVLIVLALVMIGIQWTVDTRR
                     RLAQTAATNERNQQAILRLLDELAGLADGDLTTSATVTGDFTGAIADAINYTIDQLRV
                     LVSSINQTSRNVSQAAEDTQATALHLAEAAEHQAQEIAGASSAITEMAMTIDQVSSNA
                     AESAGVADRSVALANTGSRVVQNTIGGMDMIREQIQETAKRIKRLGESSQEIGDIVSL
                     INDIADQTNILALNAAIQASMAGEAGRGFAVVADEVQRLAERSGGATRQIEALVKTIQ
                     SDTHEAVTSMEQTTAEVVRGARLAEDAGVALEEIESVSKNLAALIQNISNAARQQASS
                     AGHISNTMNIIQEITSQTSAGTTATAQSIGKLAAITNELRNSVAGFKLPDDGRGARSR
                     "
     gene            complement(12447..12977)
                     /locus_tag="PLW13_05955"
     CDS             complement(12447..12977)
                     /locus_tag="PLW13_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQY69951.1"
                     /translation="MAATAPFDLLLDISARVRSDGTEARLRMQPHWTGVAFSVLGQPL
                     VAPMGSVTEILMVPPLTRLPRVQPWVKGVANVRGRLLPVIGLAAFLGGRATLNWREHR
                     VLVLGNAGFDCAVAVDEVQGLKHFTAEPFREGAGGIVAELAPFVEGVFTAGDGDWAVF
                     RPDALVGDPRFLDAAR"
     gene            complement(12992..13366)
                     /locus_tag="PLW13_05960"
     CDS             complement(12992..13366)
                     /locus_tag="PLW13_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY69952.1"
                     /translation="MPRILVVDDSPTENFKISGVLKRHGHEVLAAESGEQALQVARDS
                     QPDLILMDIVMPGLNGFQATRQLTRDPATSAIPVVMLTAKSQEADRIWGERQGARGYL
                     VKPVEDSVLVATISQVLAEASR"
     gene            complement(13390..13779)
                     /gene="pilG"
                     /locus_tag="PLW13_05965"
     CDS             complement(13390..13779)
                     /gene="pilG"
                     /locus_tag="PLW13_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HQY69953.1"
                     /translation="MEQSLTGLKVMVIDDSKTIRRTAETLLAKEGCEVITAEDGFDAL
                     AKIADHRPDIVFVDVMMPRLDGYQTCALIKNNSEFRSMPVIMLSSKDGLFDKAKGRIV
                     GSDQYLTKPFSRNELIGAIQQHVRAGA"
     gene            13955..14905
                     /gene="gshB"
                     /locus_tag="PLW13_05970"
     CDS             13955..14905
                     /gene="gshB"
                     /locus_tag="PLW13_05970"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQY69954.1"
                     /translation="MTIRLGVVMDPIAGLNYKKDSTLALLWAAQDRGWELHYLEQQDL
                     FLRDGSARANTRALRVHRDAARWFDLGEPRELALGELDVVLMRKDPPFNNEYIYSTYI
                     LEAGARDGCLVINDPQSLRDCNEKLFATRFPQCCPPLLVSRNAAQLREFQREHGDVIF
                     KPLDSMGGTLIFRVRDGDPNISVILETLTANGATTIMAQRYIPAIVDGDKRILMIDGE
                     PAPYALARIPAAGETRGNLAAGGTGEARPLSERDRWICDEVGPALRARGLVFVGLDVI
                     GDYLTEINVTSPTCIREIDKAHGLDIGASVIAAIDARLGR"
     gene            14911..15780
                     /locus_tag="PLW13_05975"
     CDS             14911..15780
                     /locus_tag="PLW13_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY69955.1"
                     /translation="MRAWDDPALGNDRLLFCAVVALAVHAALILGVGFTREQRAPRAP
                     GLDVTLAQLRHDAPDEAADFLAQANQRGSGSANEATDPGAPVSSVFDAPAIRPVAQAP
                     GAPPEAARDPAPALTSTRSEQRIAARDPAARRAPAEPRPQAAPPAEASAEIASLVARL
                     DAQQKAYARLPRVERLTSAATRAAADAAYLYTWKQRIELIGNQHYPVEAKRRRLYGDL
                     RLLVALRPDGSVKEVRVLQSSGYALLDDAAVQIVRLSAPFEPFPPELRARADELEIIR
                     TWQFRSNRLSSSE"
     gene            15841..16401
                     /locus_tag="PLW13_05980"
     CDS             15841..16401
                     /locus_tag="PLW13_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQY69956.1"
                     /translation="MNVRLRNHFLIAMPGMEDSIFAHSITYLCEHNEHGAMGIVVNRP
                     LELSFDDIFEHLEIPGFVHRHDQPVLAGGPVQTDRGFVLHRREKRWHWQSTLHVTAQI
                     SLTTSRDILESIAHDNGPQEALIALGYAGWGPGQLEEELAANAWLSLPAEPSILFEVP
                     LEQRAQAAAARLGINLGLLSSTFGNA"
     gene            16408..16815
                     /gene="ruvX"
                     /locus_tag="PLW13_05985"
     CDS             16408..16815
                     /gene="ruvX"
                     /locus_tag="PLW13_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQY69957.1"
                     /translation="MGVVLAFDYGLRRIGVAVGNPLTGTATALPVLPARDGVPDWERI
                     RRLIGEWGPALLLVGLPLNMDGTPSAMSERAARFSRRLHGRFGIRCELMDERLSSFEA
                     RDRMAGEQGRTALDSVAACVILESWFAERSPSG"
     gene            complement(16822..17967)
                     /locus_tag="PLW13_05990"
     CDS             complement(16822..17967)
                     /locus_tag="PLW13_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQY69958.1"
                     /translation="MEVTDMLARMVEKKASDLFITAGVAPCIKVHGKLHPVDDVPLTP
                     ERARELVLSIMSDDQRREFVRTHECNFAISSRGVGRFRVSAFYQRNLVGMVLRRIESK
                     IPTVDELQLPPIINELAMTKRGLIIFVGATGTGKSTSLAAMVGYRNRNSSGHIITIED
                     PIEYVHQHNKCIVTQREVGIDTESFEIALKNTLRQAPDVILIGEIRTRETMQHAVTFA
                     ETGHLCLATLHANNANQALDRIQNFFPADARDQLWMDLSLNLKAMIAQQLIPTVDGQG
                     RRAAIEILINTPLVSDIIRKGEVHALKELMERSTELGMQTFDQSLYQLYCDGVISYEH
                     ALSYADSQNNMRLMIKMGTQTGGLGRAAGLAIDDTTENTGQFFGKRP"
     gene            complement(18047..>18228)
                     /locus_tag="PLW13_05995"
     CDS             complement(18047..>18228)
                     /locus_tag="PLW13_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HQY69959.1"
                     /translation="TPAIRNLIREDKVAQMYSAIQTGSGVGMQTMDQCLTDLVAKRLV
                     TREAAREKAKIPDNF"
BASE COUNT         2691 a         6470 c         6271 g         2796 t
ORIGIN      
        1 cgcaagatca tcgtcgacag ctacggcggc atggcgcgcc acggcggcgg cgccttctcc
       61 ggcaaggatc cctcgaaagt cgaccgctcg gccgcctatg ccgggcgcta cgtggctaag
      121 aacatcgtcg ccgcgggcct cgcggagcgc tgcgagatcc aggtttccta cgcgatcggc
      181 gtggccgagc cgacctcgat ctcggtgaac accttcggta ccggcaagct gtcggacgcg
      241 gcgatcacgg acctgatccg ccagcatttc gacctgcgcc cgcgcggcat catcgagatg
      301 ctcgagctgc gccgcccgat ctaccgtgcc accgcggcct acggccactt cggccgcgag
      361 gaacccgact tcagctggga gcgcaccgac aaggccgcgg cgctggcgaa ggcactttga
      421 actggacgga gcctttgcaa cgtagcacta gcgaggcgtg gtcgggggct gcaaggcttt
      481 tcccgacacg ccgtgaatac atccctgtag gctcgagcgc cgcatccctg cggcgcacgg
      541 tcctggaaaa gccttgcggc ccccgaccac ccgtgccgcc tggattgccg caacatcgcc
      601 acgacttcag aggaaatgac agatgaccac cgcacagaaa gcattcaccg actacaaagt
      661 ggccgacata tcgctggccg cctgggggcg tcgcgagatc gcgatcgccg agagcgagat
      721 gccggcgctg atggcgatcc gcgcccgcta ccgcgacagc cagccgctga agggcgcgaa
      781 gatcctcggc tgcctgcaca tgacgatcca gaccgccgtg ctgatcgaga cgctggcgga
      841 actcggcgcc gagctgcgct ggtcctcgtg caacatcttc tcgacgcagg accacgccgc
      901 cgcggcgatc gcggcttcgg gcgtcgcggt gtacgcgtgg aagggcgaga ccgaggagga
      961 gtacgagtgg tgcatcgagc agaccatcct gaaggatggc aagccgtggc cggtgaacat
     1021 gatcctcgat gacggcggcg acctcacggg catcctgcac gagcgctacc cgcagctgct
     1081 ggacaaggtg cacggcatca ccgaggagac caccaccggc gtgttgcgcc tgcaggagat
     1141 gctcaagcac ggcacgctga aggtgccggc gatgaacgtg aacgactcgg tgacgaagtc
     1201 caagaacgac aacaagtacg gctgccgcca cagcctgagc gacgcgatca agcgcggcac
     1261 cgaccacctg ctcgccggca agaaggcgct ggtgataggt tacggcgacg tgggcaaggg
     1321 ctcggcgcaa tcgctgcgcc aggaaggcat gatcgtgcgc atcagcgaga tcgacccgat
     1381 ctgcgcgatg caggcctgca tggacggcta cgaagtggta tcgccctacc tcgacgggcg
     1441 caacgacggc agcgccgagg gcatcgaccg ccagctgctg gcgcagaccg atctggtggt
     1501 caccgccacc ggcaacatgg atgtctgcga ccaccacatg ctggcggcac tgaagtgcgg
     1561 cgccgtggtg tgcaacatcg ggcacttcga ccacgagatc gacactgcct tcatgcgccg
     1621 caactgggag tgggaggaag tgaagccaca ggtgcacatg atcttccgca accgcgccac
     1681 caacgaccac ctcatcctgc tctccgaggg ccgcctggtg aacctcggca acgccaccgg
     1741 gcacccctcg cgcatcatgg acggctcctt cgccaaccag gtgctggcgc agatctacct
     1801 gttcgagcgc cgtttcgcgg acctcgagcc ggaaatgaag ccggccgcgc tgagcatcga
     1861 ggtgctgccc aagcagctcg acgaggaggt cgcccgccac atggtcgccg gattcggcgg
     1921 cgtgctcacg cgcctcacgc cgcagcaggc cgactacatc aaggtgccgg tcgaaggacc
     1981 gttcaagaac gaacagtacc gctactgagc gaggaccatg ggcaactcat cccgagcctt
     2041 gcgcctgagc tgcgagtact tcccgcccaa gaccgaggac gggaagcaca agctgctggc
     2101 ggcgcacgac aagctgcagg cgctgaagcc ggagttcttc tccgtgacct acggcgccgg
     2161 cggctccacg cgcagcaaca cgcgcgacat ggtgctggaa ctgaagcgcc gcggcagctg
     2221 tgtcgcgccg cacctgtcct tcggcggtga cgacgaaagc ctggtgctgg aactgctgca
     2281 ggtctaccgc gacaacggca tccgccgcct ggtggcgcta cgcggcgaca tgccctcggg
     2341 catgggcggc atgtcgcaga tggtgtacgc cgccgagctg gtcgggttcg tgcgcaggca
     2401 caccggcgcg cacttcacga tcgaggtcgc ggcgtatccg gagatgcacc cgcaggcgcc
     2461 cgacatgatc gcggacgtgc gattcctgaa gcgcaagttc gacgccggcg ccgacagcgc
     2521 cctcacgcag ttcttctaca acgccgacgc ctacttccgc ttcatcgacg cgtgccgtgc
     2581 cgagggcatc acgcaaccgg tctatcccgg catcatgccg atcctgaact accagaacct
     2641 cgcgcgcttc gcgcgcaact gcggcgccga gatcccgcgc tggctgcact accggctgga
     2701 atcgctggaa agcgatgccg ccggcacgcg cgagttcgcc gaggagttcg tggcctcgct
     2761 gtgccgccgg ctgctcgagg gcggcgcgcc gggactgcat ttctacacca tgaaccaggc
     2821 cgagccgacg ctcgcgatag cccgcggggc gggactgctc tgaggatgcg cgcaccgcgg
     2881 ctgttcgtcg agaccgcgct cgcgacgggc gcggcgatcg aactgaagga cgaggcgctg
     2941 cactacgcgc gcaacgtgct caggctcgat gtcggcgcgc cgctgctgct gttcaacggc
     3001 gagggcgcgg agttcgaggc cgcggtcacg gcggcgggca agcgatcgct cacgctggcc
     3061 gtgggcatcg ataccggtgc tgcgcgcgag gccacgctgg cggtgcatct gggcatcggc
     3121 ctggcacgcg gcgaacgcat ggactgggtg gtgcagaagt gcaccgagct cggcgtcgcc
     3181 tcgatcacac cgctgctgct cgcgcgctgc aacgcgaagt tcgaccaggg ccgcggcgac
     3241 aaccggcggc gccactggcg ccaggtcgcg atcagcgcct gcgagcagtg cgggcgcaac
     3301 cggctgccgt tgatcgggga gcccgtgacg ctcgatcact ggcttgccac acgcaccgag
     3361 gcacccggct tcgtgctgca caccgccgac agccatgcgc tcgcccgggg cgacgcgccc
     3421 gcggcggtgc gcctgctggt gggccccgag ggcgggctcg acagcaacga attcgcgcag
     3481 gcccgcagcg ccggcttcac gccatggtcg ctgggtccgc gcgtgctgcg aaccgaaacg
     3541 gcgccagtgg cggcgctggc gatactgcag tacctgtggg gtgatctcgc gtaggtccgg
     3601 atttatccgg acgatgtccg cacgaatgcg gacctacggt gcggcgaggt gctgctcgat
     3661 gaaatcgaaa tccggtatcg cggcgtcctg accgtgcacg cgaaccttca tgcgctcggc
     3721 cggacccggc gccggcgcgt ccacggcctg cacatcggcg gcggtcagca gcagcaggtg
     3781 cggcacgccc tgggcgagga tcgcgtagtt gcgcgcgggc gcggcatcgg acagcgtgct
     3841 caggatcacg atccggctgc cgtgcccggg atcggggccg gcatgcccgt tcagcgcctc
     3901 gaaggagagc acgggcagtt tctcgtggtg ccacaacagc gtgcccagca gccagtcggg
     3961 cgccgccggc gggcgctgca gatcgcggcg cttgatgatc tcggccacca cggcgccggg
     4021 aaccaccagc agttgtccgg ttaccgggac cagcagcgcg ctcaggtccc gcgggcctgg
     4081 caccgcgttc atgacgacgc tccctggcgc gcgagcagcg catggatatg ctccagcagc
     4141 accgactcct ggtagggctt gccgaggtac atgttcgccc ccagcgacag cgcgcgctcg
     4201 cgatgcttgt cgccggtacg cgaggtgatc atgatgatcg ggatgcggcg cagctgctcg
     4261 ctcaggcgca ccttgctgac cacctcgaag ccgtccatgt gcggcatctc gatgtcgagc
     4321 agcatcacgt ccggcacctg ttcctggagc ttcgccatcg catccgcgcc gtccttggcc
     4381 gtgatcacct gcatgccctc gcgctcgagg aagcgcgtgg tgaccttgcg cacggtcacg
     4441 gaatcgtcca ccaccatgac cagcggcggg cgctcggcct tgtgctcctg cgcgccgcgc
     4501 ggcgcgtgtt cgtagccggt ggcgccgtgc gccgcatcgg cacgcagcat cgcggggatg
     4561 tcgaggatca cgaccacgga gccgtcgccg agcaccgttg cgccgctcaa ccccggcacg
     4621 gcgccgaact gcggccccag gctcttgacc gcgatctcgc gcgcgccgac cagccggtcg
     4681 acctgcaccg cgagcgcctg ccctccaccg cgcaccagca ccacgggcac cgtgtcggaa
     4741 aggctgcccg ggtcgggctg ctcgtcgggg tagagcacgg cacccaggtg gcgcacctgg
     4801 tagctctggc cggcatactc gaacggggcc gcgtcgggac cggcgtagtg gcgcagctcg
     4861 tcggcacgca tgcgcaccac gccttccacg gagttcagcg gcagggcgtg gatttcgggg
     4921 cccaccgtga ccagcagcgc gcggctcacc gacaccgtga acggcagccg caccacgaag
     4981 cgcgtgcccc ggccgggctc gctgtggatt tccagcgagc cgcccatctg gcggatctcg
     5041 ctgctgacca cgtccatgcc cacgccacgg cccgagatct gcgtgaccgc ggcggcagtg
     5101 ctgaagccgc tcgccaggat gaactgcagg atctcgtggt cgccgagctg cgcatccggc
     5161 tccatcagcc cgcgctcgac cgccttggcg cgcacggccg cgagatcgac gccgccgccg
     5221 tcatcggcca ccacgatcag cacgtcgccg ccttcgcgcg cgacctcgat gccgatgtgt
     5281 ccggtcgcgg gcttgccgcg ttcgcgccgc agctccgcgg tctcgatgcc gtggtccacc
     5341 gcgttgcgca gcatatgctc cagcggtgcc accacgcgct cgaggatgcc gcggtccagc
     5401 tcgccggcga tgctgccgag ctccagctcg acctgcttgt gcagctcgcc cgccacctgg
     5461 cgcaccacgc gcttgagtcg cggcaggatg ctcgagaacg gcaccatgcg cgagctcatc
     5521 agcccttcct gcagctggct gttgatgcgt gattgctgga taagcaccgt ttcgaggtcg
     5581 cgcgtcttct cggccagcgt gcgcttgatg tcgagcaggt ccgaggcgct ctcgaccagc
     5641 gagcgcgaca gctgctgcag ctgcgagtag cggtccattt ccagcgggtc gaagccgtcg
     5701 acggctgcgt tctccgcccg ttcgcggcgg aacagcacct gcgcctcggt cgcgatgtcc
     5761 aggcgccgga cctggctctg cagccgctcg atggcgccct gcatgtcctc gaacagctgt
     5821 cccagctcgc tgacctgctc ctcgacgcgc ccgcgcgaga tgctggtctc ggcggcgaga
     5881 ttgaccaggt cctcgagcag ttgcgccgat acgcgcacca cttcgtcgcg cggcttcgtc
     5941 gcgccgggcg gcaccgccac ggcgggagcg ggtcgccgcg gcgccacgcc cgggggtacg
     6001 gcggcgagcg gcgcggccgg cgccacctcg cttgcgactc gctcggcagg cgccaccggc
     6061 tcggcctgct ccggcgctgc cgggaccgct tcggccacgg cagcgagaac cgcgggcgcc
     6121 gccaccgggc gctccttgac cacgtccatg gtgcgcagca gctgctcgtg gaaatccgcg
     6181 acgcgtgcga agaaggcgtc gtcgaaggcc ccgaagcgta ccgcctggtt gatcaggaag
     6241 gtctcgaagt tgtggctgag ctcgccgagg tacctgagcc ccgccatgcg ggcgccgccc
     6301 ttgagcgtat gcaggtggcg ctgcaggtca tcgaagggcg cgcggctgcc gcggtcgtga
     6361 cgccaggcct cgatcgactc gtcgatcgcg cccatgagtt cctcggcctc ctccatgaag
     6421 atgccgagca attcgtgctc ggcatgctgc acgtcgcggc cgacgcgcgc acgcagcagt
     6481 tcctcctcgg cctcgccgcc ggcgcgctgg ggcgccggca cgggcccggg tgcggactcc
     6541 ggtgcggcct gggccgccgc ggcttcgtga agttgcgcga tgatcgcctg cggcgcctcc
     6601 ggcagctgtc cggcggcgag gcgatccatg acttcgagga aggcttccag cccgcgcctg
     6661 gccagctcga agagttcggc cgcaggacgt gtcacgccgt gcgcagcagg gcgcaacgcc
     6721 gcgagcagcg gtcgcgacag ctcgagcatg ccggcgatat tcagctgctc cgcgcgctgg
     6781 tcgatgacct gcaactcctc gagcagggtg cggcgtgaca cctcggcgtc gcggtccgcg
     6841 cgccacgcct cgagcagcgt ggtggcgtgg aacagctttt ccagcgcttc ctcgagaaag
     6901 cgcgccagcc gcgcgtcgag cgctgcagcc ggcacccggg cgggctcggt gcgcgcgcag
     6961 gcgagcacct gctcgcgcaa tgccgcggcg tcggccgaca attgcgcgct gtcgcgcgcg
     7021 cccgcggcca gcacggcgtc gagcagcgtg cacagctgct gcagctggtc gagctccggc
     7081 gcctgcaggg cacgctcggc ctccagcgcg gcacgcacca gctcgtccac ggcatcgagc
     7141 acccgtccga gcgcctcgtt gccggcggcg agcgcgctgc cgcgcagcgt gtgcagggtc
     7201 cgcagcagca gttgcgtgac cgtgccgcgc tccgcgccag cgaggtagtc gcgcagggcg
     7261 cgcagatgtt ccgcgagctc ctcggcgaag atcgcatgca ggcgtgcggc ctcttcgtct
     7321 gcggccgctt cgacaacggg ttcggactcc ccgggcgaca tctcctcgac gaactccggc
     7381 tccgccccgg cttccggcaa ccattcctcc ggcgccgcca cttgcggcaa cagctcctcc
     7441 gccaccgact cctgcagttc cgcgcccggc agccaggctt gctctgcgct ctccacgggc
     7501 aacagctctt cggccagcgg ctcctgcggc gcctcgaccg gtgtgagcgc ccactgcgat
     7561 ggttcacgcg tcgtcagcga ctccactgtc tcctgcagcg cccgcaccgc ggcggcatcc
     7621 accgccgcgc cgccggcgaa cgcctgcacc agcgcgggca gctgctgctg cgcgcgttcc
     7681 actgcctcca tcactgcggg cgtgggctcg agcgcgcgtt ccgtcacgcg gttgagcagg
     7741 tgctccaccg accacgacag atcgccgacc agctccgccc ccaccatccg cccgctgccc
     7801 ttgagcgtgt ggaaggcccg gcgcaattcc tcgagcggcg caccggcaag cggcgcggcg
     7861 cgccacagcg gcagctgcgt ggcgatgcgc tccagcacct ccgccgcttc ctcggtgaag
     7921 atcgccgcga tctccggatc cggcgcctgc ccttcggctt gcccggccag ggactccggc
     7981 tccgccacca gctcctcggc gagttccggc accgcctcgg cctccggcag ccattcctcc
     8041 ggcgcaagcg tcccgggcag caactcttcg gccacctgtt cgtgcggtgc ctcggccgca
     8101 gtgggcgccc actgtggcgc ttcgccgcgc gtcacggcct gcaccgccga ctgcagcgtc
     8161 cgcaccgtgc tttcgtccac gctcggcccg ccggcaaagg cccgcaccag cgccggcagc
     8221 agctgatgcg cccgctccac cgcctcgacc accgcggacg agggttcgag cgtgcgctcg
     8281 atcacgcggt tgagcagctg ctccaccgac cacgacagtt cgccgatgtg cgcggcgccc
     8341 accatccgcc cgctgccctt cagcgtgtgg aaagcccggc gcagctccgc cagcggcgca
     8401 ccgccgcccg gtgccgagcg ccacggcggc agctgcgccg cgatggtttc cagtacttcc
     8461 gccgcttcct cggtgaagat ctccacgatc tccggatccg ccactgcgac ggcgtgctcc
     8521 gcggcgacga gcgccgcggg cgctagcggc tgctccggct cggcaggcgc cggggcgcct
     8581 gcttcctgcg cgacggcgag cggcactgcg gttgcggcga tcgccacctc gggctgcggc
     8641 gcgcccggcg cctccacgag cccggcgact tgcggcaacg ccgcgagccg cgcgagggat
     8701 tcccgcgccc gccccaggat cagttctgcc gggacctggt cgatggcgag ccgctcgagg
     8761 aaatactcga cgctgccgag cgcgtccgcg agcgcctcga cagcctggct gtcgggcgcc
     8821 gcacgcgagc ccgccagcac ggcgccgaga tgatcgcggc attcgcccag cacctgcgcc
     8881 gccccgtcca ggcccgccac gcgcagcgcg ccggcgagcg cgtgcaggcg ctccggagcg
     8941 ccggcaagca gcgtggtatc ggcggccgac gtgacgtaat cgccgacggt gcgcttgacc
     9001 tcttcgaggc cggcccggac ctcgcggtaa accgcggcca gcgcgcgctg cagcggatgg
     9061 cgccgcgact cggccagttc gtcctgctcg ccgcgcgcct gcacggcgcg cgaggccagc
     9121 cgctggtcga cgtcgagcag ggcggcggaa atctcggtga gccggtcgtc gaggtccgcg
     9181 ccgccgacca gcccggactg gatgcgctcg agttgcggct gcaggatctc gtgctcttcc
     9241 tggtagccga gcccgtccag cagctggccg acgcggcgca gatccggctc cacctcgcgc
     9301 agcacgcgcg agatgctgaa ggcatcaccc tcggccgcct cgagccgtgc gagcaccgcg
     9361 ctcagctcgg ccgcgagcac cgcaaccgcc gcaccggtgg cccccaggtc gtcgagcgtc
     9421 gcgggcccgg ccgccacggc cgcgtcgggg gtcaggcggt ggcgttgctg gatttcggcg
     9481 agcagcgccg agaccggccg gctctgcgcg atcaattcca gcagccggcg gtagagcccg
     9541 gcatcgggcg tgcggcgata caggccatgc tcgagcgcga cgcccgcacg caactcgccg
     9601 tcgagctcgc gcagcaccgc gatggccgcc tcgtcgagcg cgacaccccc ctggtccagc
     9661 gcctcggcca gcgcggcgca ggcctgccag aacaggcggt gtccgccggg gcgcaggcgc
     9721 tgcgccagca gcgcgaacag cttgcccatg ttgcgcaggt gctcgcccgt tgcctcgtga
     9781 cgcagcacgc ccagcagctc ggcctggaaa cgctggcgcg caagccgcag cagctgcacc
     9841 tcctcgtcat cggcctgcgc cgccgacaca ccgctgcctt gcgcgagctc cgcggtgtcg
     9901 atcgtgtcgg ccagcggcgg tgccgccagg ggcgcctcgg tgcgcgcagc gcgcagctcg
     9961 ttgaccaggc cgagcacgtc gagcggtgat tcgcgccgct cggcggcgac gcggtgcagg
    10021 taggcgggca actgcatgcc cgcggccagc aacagctgca ggttggcccc ggagggctcg
    10081 atgcgcccgt gcagcatggc ctgcaccgtg tcctcgatct cgcgcgcgag ctgctcggcg
    10141 ctcggcacgc cgagcagccg cagcgtggcg tgcagctggt gcgcctgggt caggccgagg
    10201 cgcaggcggg tctggtcgga cacgttgtcg gcaaagccga gcacggcctt gccgaactgc
    10261 ccggcgatct cgtcgatctc gccgccgacc cactgcaggg ccacgaaatc ctgttgcatc
    10321 gcgctggcgc cccggttcag cggctgcggg cgccgcgccc gtcatccggc agtttgaaac
    10381 cggccacgga gttgcgcagt tcgttggtga tcgcggcgag cttgccgatc gactgtgccg
    10441 tcgcggtggt gccggcggag gtctgcgagg tgatctcctg gatgatgttc atggtgttgg
    10501 agatatggcc ggcagaggag gcctgctggc gcgcggcatt cgagatgttc tggatcagcg
    10561 ccgccaggtt cttcgacacc gattcgatct cctcgagcgc cacgccggcg tcctccgcca
    10621 gacgcgcgcc acgcaccact tccgcggtgg tctgctccat cgaggtcacc gcttcgtggg
    10681 tgtcggactg gatcgtcttc accagcgcct cgatctgccg ggtcgcgccg cccgagcgct
    10741 cggcgagccg ctgcacctcg tcggcaacca ccgcgaagcc acgcccggcc tcgccggcca
    10801 tcgaggcctg gatcgccgcg ttcagcgcga ggatgttggt ctggtcggcg atgtcgttga
    10861 tcagcgacac gatgtcgccg atctcctgcg agctctcgcc gaggcgcttg atgcgcttgg
    10921 cggtctcctg gatctgctcg cggatcatgt ccatgccgcc gatggtgttc tgcaccacgc
    10981 gcgagccggt gttggcgagc gccaccgagc ggtcggcgac accagccgac tcggccgcgt
    11041 tggaagacac ctggtcgatg gtcatcgcca tctcggtgat cgcggacgag gcgccggcga
    11101 tctcctgggc ctggtgctcg gcggcctccg cgaggtgcag cgccgtggcc tgggtgtctt
    11161 cggcggcctg cgagacgttg cgcgaggtct ggttgatcga ggacaccagc acgcgcaact
    11221 ggtcgatggt gtagttgatg gcgtcggcga tcgcgccggt gaagtccccg gtgaccgtgg
    11281 cgctggtcgt gaggtcgccg tcggcgagcc ccgcgagctc gtcgagcagg cgcaggatgg
    11341 cctgctggtt gcgctcgttg gtcgcggcgg tctgcgccag gcgccggcgc gtgtccaccg
    11401 tccactggat gccgatcatc accagcgcca gcacgatcag cacgccgaag gcgagcgcgc
    11461 tgcccatgtt gagcttgcgc tcgccctcga ggtcgttgat gcgatcggag gtcacgctgg
    11521 tggcgttgag cagcttcggc gcgctctgca cgatagcctc ggcggcctgg cgcgcctgct
    11581 tgagatcggg cgcggaatcg aggatgcgct gcttgccgcc gccgatgacg cgaaacagct
    11641 ccttcacctc gcgcgcatcg gccagcgccc cggcgttgac gagcgccggg atgcccatcg
    11701 cggcgctgcc ctcgagcatg ccgtcgagca cgtcggcgaa gctctcggtg tcggcgttga
    11761 actgctcggc ggcctcggtc gcgccactgc cgccctcgag catgcggtcg agattgcgcg
    11821 agatgcgctc tgccagccat gactgacgct cggcgagcgc cacctggcgt attgtcgccc
    11881 cgctgccggc gaggacgtcg gcgatgcggg ccgtgcgctt ttgcacgtcc tcgatggaac
    11941 ccgcgagggt cgccgaggtc ttgctgagga actcgatact gtccttgcgc gcgacgatgg
    12001 tcgacgcggc gggatcgacc gctgaccagg tctcgccgat cagcgccagc tcctcggcga
    12061 gagcctcgcg cggcgagggc agggcatccg tgccattggc cagatcgtcg agggtgcggt
    12121 cgaaatcggc gcgcgcgctc tccaggttgg tgaacgccgc cgcgtccccg tccacggcct
    12181 cgcgcgcatt ggcctcgatc tgctgcgaca gcacgcgcag gttgccgacc agctcgcggt
    12241 agccggcgtc gcgattggtg tcgcgcatga tgacgaagag attccagacg aaggccagca
    12301 cggccaccac gaggacgcct ccccacacca ggatccaccg gacattcggc tggctggcgg
    12361 ccggcacgtt ctgcttcgcg ttcatctgac tcccatccta gtggcaatgc atgaccgtgc
    12421 ccgtgcggcg cgggcggtgg ccggcgctag cgcgccgcgt ccaggaaacg cggatccccg
    12481 accagcgcat cggggcggaa cacggcccag tcgccgtcgc ccgcagtgaa gaccccctcg
    12541 acgaagggcg cgagctccgc gacgatgccc cccgcgccct cgcggaacgg ttcggcggtg
    12601 aagtgcttga gtccctgcac ttcgtcgacg gccacggcgc aatcgaagcc ggcgttgccg
    12661 agcaccagca cgcggtgctc gcgccagttc agcgtagccc gtccgccgag gaacgccgcg
    12721 aggccgatga ccggcagcag ccgcccgcgc acgttcgcga cccccttgac ccatggctgc
    12781 acccgcggca gtcgcgtcag cggcggcacc atcaggattt cggtgaccga gcccatcggc
    12841 gcgaccagcg gctgcccgag caccgagaat gcgacgccgg tccagtgcgg ctgcatgcgc
    12901 aggcgtgcct cggtcccgtc gctgcgtacg cgcgcggaga tatccagcag caggtcgaag
    12961 ggagcggtgg ctgccatcgc gcgggcccgc ctcagcggct ggcctcggcc agcacctgcg
    13021 agatcgtggc aacgagcacc gaatcctcga ccggcttgac gaggtaaccg cgcgccccct
    13081 ggcgctcgcc ccagatgcgg tcggcctcct ggctcttggc agtcagcatg accaccggta
    13141 tggcggatgt cgccggatcg cgcgtgagct ggcgcgtggc ctggaagccg ttcaggcccg
    13201 gcatcacgat gtccatcagg atgaggtccg gctggctgtc gcgcgcgacc tgcagggcct
    13261 gttcgcccga ttcggcggcc agcacctcgt gtccgtggcg cttcagcacc ccgcttatct
    13321 tgaagttttc cgtcggcgag tcatcgacga ccaggatccg cggcatcatc tgtctcctgc
    13381 gcggcgcgct caagcgcccg ccctgacgtg ctgctgtatc gccccgatca gctcgttgcg
    13441 gctgaagggt ttggtgaggt actggtcgga accgacgatg cgccccttgg ccttgtcgaa
    13501 caggccgtcc ttgctggaga gcatgattac cggcatcgag cggaattcgc tgttgttctt
    13561 gatcagcgcg caggtctggt agccgtcgag acgcggcatc atcacgtcga cgaagacgat
    13621 atccggccgg tggtcggcga tcttcgccag cgcgtcgaag ccgtcctcgg cggtgatgac
    13681 ctcgcagccc tccttggcga gcagcgtctc ggcggtgcgg cggatcgtct tgctgtcgtc
    13741 gatgaccatg accttgaggc cggtcaagct ctgttccatg gcgaggatcc ccgataatgg
    13801 ccctgggtct gttcccgcgg ttcccgcgac ggcgtcgcgg cgcgttcagc aagcggccgg
    13861 gcttctgcta tcgttgtccg gcggtcgtca attaatagac catgggcgtg cggaaataca
    13921 ggcaggcctc ccaaaatccg gacggagttc gcagatgaca attcgcctcg gcgtggtgat
    13981 ggatcccatc gccggactga actacaagaa ggacagcaca ctggcgctgc tgtgggccgc
    14041 gcaggaccgc ggctgggagc tccactacct ggagcagcag gacctttttc tgcgcgatgg
    14101 cagcgcgcga gcgaacacgc gcgccctgcg cgtgcaccgc gacgcggcgc gctggttcga
    14161 cctcggcgag ccgcgcgagc tcgcgctcgg cgagctcgac gtggtgctga tgcgcaagga
    14221 tccgccgttc aacaacgagt acatctactc cacctacatc ctcgaggccg gcgcgcgcga
    14281 cggctgcctg gtgatcaacg atccgcagtc gctgcgcgac tgcaacgaga agctgttcgc
    14341 gacccgcttc ccgcaatgct gcccgccgct gctggtgagc cgcaacgccg cgcagttgcg
    14401 cgaattccag cgcgagcatg gcgacgtgat cttcaagccg ctcgactcga tgggcggcac
    14461 gctcatattc cgcgtgcgcg acggcgaccc gaacatcagc gtgatcctcg agaccctcac
    14521 cgccaacggc gccaccacga tcatggccca gcgctacatt cccgcgatcg tcgacggcga
    14581 caagcgcatc ctcatgatcg acggggagcc cgcgccctac gcgctggcgc gcatcccggc
    14641 ggccggcgag acgcgcggca acctggccgc cggcggcacc ggcgaggcgc gaccgctcag
    14701 cgagcgcgac cgctggatct gtgacgaggt gggcccggcg ctgcgcgcgc gcggcctggt
    14761 gttcgtgggg ctggacgtga tcggcgacta tctcaccgag atcaacgtca ccagtccgac
    14821 ctgcatccgc gagatcgaca aggcgcacgg gctggacatc ggcgcgagcg tcatcgccgc
    14881 gatcgacgcg cgcctggggc gctgagcccg gtgcgagcct gggacgaccc ggcgctcggc
    14941 aacgaccggc tgctgttctg cgccgtggtg gcgctggccg tgcacgcggc gctgatcctg
    15001 ggcgtgggct tcacgcgcga gcagcgcgcg ccgcgcgccc cggggctcga cgtgacgctg
    15061 gcgcagctgc gccacgacgc gccggacgag gccgcggact tcctcgcgca ggccaaccag
    15121 cgcggcagcg gcagcgccaa cgaggcaacc gacccgggcg ccccggtcag cagcgtcttc
    15181 gatgcgcctg cgatacggcc ggtggcgcag gctcccggcg cgccgcccga ggccgcccgc
    15241 gatccggcac cggcgctgac gtccacccgc agcgaacagc ggatcgccgc gcgcgatccc
    15301 gccgcgcggc gcgcgccggc cgagcctcgg ccgcaggcgg cgcccccggc cgaggcgagc
    15361 gccgagatcg cctcgctggt ggcgcggctc gatgcgcagc agaaagccta cgcgcggctg
    15421 ccgcgcgtcg agcgtctgac ctcggcggcg acgcgcgcag ccgccgacgc cgcctatctc
    15481 tacacctgga agcagcgcat cgagctcatc ggcaaccagc attacccggt ggaggcaaaa
    15541 cgccgccggc tttacggcga cctgcgcctg ctggtcgcgc tacgcccgga cggcagcgtc
    15601 aaggaggtgc gcgtgctgca gtcctcgggc tacgcgctgc tcgacgatgc cgcggtgcag
    15661 atcgtgcggc tctcggcgcc gttcgagccg ttcccgccgg agctgcgcgc gcgcgccgac
    15721 gaactcgaga tcatccgcac ctggcagttt cgcagcaacc gcctgagctc cagcgagtga
    15781 gcgcccgggc gcgggcgcgg tggcttgcca tcattgcacc ccggggccga caatgcggcc
    15841 atgaacgtgc ggctgcgcaa ccatttcctg atcgcgatgc cggggatgga ggattccatc
    15901 ttcgcgcaca gcatcaccta tctgtgcgag cacaatgagc acggcgcgat gggcattgtc
    15961 gtgaaccggc cgctggagct gagcttcgac gatatcttcg agcacctgga gattcccggt
    16021 ttcgtgcacc gccacgacca gccggtgctg gccggcggcc cggtgcagac cgatcgcggt
    16081 ttcgtgctgc accggcgcga gaaacgctgg cactggcaat cgacgctgca cgtgaccgcc
    16141 cagatcagcc tcaccacgtc gcgcgacata ctcgagtcga tcgcccacga taacggcccg
    16201 caggaggcgc tgatcgcgct gggctatgcg ggctggggtc cggggcagct ggaggaggaa
    16261 ctcgcggcga atgcgtggct cagcctgccg gcggagccct cgatcctgtt cgaagtgccg
    16321 ctggagcagc gcgcgcaggc cgccgccgcg cgcctgggga tcaacctcgg gctgctcagc
    16381 agcaccttcg gcaacgcctg aaccggcatg ggcgtggtgc tggccttcga ttacggcctg
    16441 cgtcgcatcg gcgtggcggt gggcaacccg ctcaccggca cggcgaccgc gctgcccgtg
    16501 ctgccggcgc gcgacggcgt gccggactgg gaacgcatcc gtcgcctgat cggcgaatgg
    16561 ggacccgcgc tgctgctggt cgggctgccg ctcaacatgg acggcacgcc cagcgcgatg
    16621 agcgagcgcg cggcgcgctt ctcgcgccgg ctgcacggac gcttcggcat ccgctgcgaa
    16681 ctgatggacg agcgcctcag ttccttcgag gcgcgcgatc gcatggcggg agagcagggg
    16741 cgcacggcgc tcgacagcgt cgcggcctgc gtgatcctgg aaagctggtt cgccgaacgc
    16801 tcgccaagcg gctgagcgtc ctcagggacg cttgccgaag aactgcccgg tgttttccgt
    16861 ggtgtcatcg atcgcgagcc cggccgcacg gccgagacca ccggtctgcg tgcccatctt
    16921 gatcatcagg cgcatgttgt tctgcgaatc ggcgtaggac aaggcgtgct cgtaggagat
    16981 cacgccgtcg caatagagct ggtagagcga ctggtcgaaa gtctgcatgc cgagctcggt
    17041 cgagcgctcc atcaattcct tcagcgcgtg cacctcgccc ttgcggatga tgtccgagac
    17101 cagcggcgtg ttgatcagga tctcgatcgc agcgcgccgt ccctggccgt cgacggtggg
    17161 gatcagctgc tgggcgatca tcgccttcag gttcagcgac aggtccatcc acagctggtc
    17221 gcgcgcgtcg gcggggaaga agttctggat ccggtccagc gcctggttgg cgttgttcgc
    17281 gtgcagggtc gcgagacaga ggtggccggt ctcggcgaag gtcacggcgt gctgcatggt
    17341 ctcgcgcgtg cggatctccc cgatcaggat cacgtcgggc gcctggcgca aggtgttctt
    17401 cagcgcgatc tcgaagctct cggtgtcgat gccgacctcg cgctgcgtga cgatgcactt
    17461 gttgtgctgg tgcacgtact cgatcgggtc ctcgatcgtg atgatgtggc ccgagctgtt
    17521 gcggttgcgg taaccgacca tggcggcgag cgaggtggac ttgccggtgc cggtcgcgcc
    17581 gacgaaaatg atcagcccgc gcttggtcat cgccagctcg ttgatgatcg gcggcagctg
    17641 cagctcgtcg acggtgggaa tcttcgactc gatgcgccgc agcaccatgc ccaccaggtt
    17701 gcgctggtag aatgcgctga cacgaaagcg tcccacgccg cgcgaactga tcgcgaagtt
    17761 gcactcgtgc gtgcgcacga attccctgcg ctggtcgtcg ctcattatgc tcagcaccag
    17821 ctcgcgcgcg cgctcgggcg tgagcggcac gtcgtcgacg ggatgcaact tgccgtgcac
    17881 cttgatgcag ggcgcgacac cggcggtgat gaaaaggtcg gaagccttct tctcgaccat
    17941 ccgcgccagc atgtcggtga cttccagttc cctcttcgct gtctcgttgg cttccatgac
    18001 gggtctctcc gggggggtcg tgcgggtgtg gctgcggcga cagcgatcag aagttgtcgg
    18061 ggatcttggc cttctcgcgc gcggcctcgc gcgtgacgag gcgcttggcc accaggtcgg
    18121 tgaggcactg gtccatggtc tgcatgccca cgccggatcc ggtctggatc gcggaataca
    18181 tctgcgccac cttgtcctcg cggatcaggt tgcggatcgc cggggtgc
//
LOCUS       DAORUQ010000046        18180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2722_length_18180_cov_6.621241, whole genome shotgun sequence.
ACCESSION   DAORUQ010000046 DAORUQ010000000
VERSION     DAORUQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_18180_cov_6.621241"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLW13_06000"
     CDS             complement(<1..305)
                     /locus_tag="PLW13_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQY69960.1"
                     /translation="MSHHVDPSREHFAAFKALPRDTPILMLNLIRLRADAAYPDGRGG
                     SGKAAYAAYSRESGPVFQRVGGRIVWRGRPEQVVIGPPEERWDIAFIARYPTAAAFL"
     gene            complement(318..3044)
                     /gene="ppdK"
                     /locus_tag="PLW13_06005"
     CDS             complement(318..3044)
                     /gene="ppdK"
                     /locus_tag="PLW13_06005"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQY69961.1"
                     /translation="MPADSSARYVYLFSEIDAVRASAGGDWQAVRALLGGKGANLAEM
                     SSLGIPVPPGFTISTAACRDFLAADARLPGTLWEEVLAAVRAIESATHKRFGDRDNPL
                     LVSCRSGARQSMPGMMDTILNIGLNDEVVAGLARRSGDERFAFDLYRRLVQMFGSVVM
                     GVPDEVFDAVITAQRNRLGVASDAGMDAGHWRLITARFKEIYRTYTGADFPSDPYEQL
                     RLATEAVFRSWNGKRAVDYRNAAGIPHDLGTAVNIQTMVFGNMGANSATGVAMSRNVS
                     TGERELEGDFLFDAQGEDVVAGIRLTQPLAELAQQMPEIYRDFAGIAARLEMHYRNVQ
                     DMEFTIEQGRLWLLQTRDAKRTAQAEVRIAVDMVEEGLITREEALRRVRPEQVDFFLH
                     PQLEPDAVRRAELIGSGLNVSPGAAVGMVALDAALAQRWGRDEDKPVIMVRPETKPDD
                     VHGMLAARGVVTSRGGRTSHAALVARQFGKPAVVGIEALEIDLERREMRLGDVVVREG
                     DWISIDGSNGLVYVGKLPTLVPDLSNTYLIRLLEWADEVRRLEVWANADYPRDAARAR
                     LYGAQGIGLCRTEHMFFEAERMPLVQRMIMAREPSERNEAIAALLPPQRSDFEGLFRA
                     MEGRPVTIRLLDPPLHEFLPAYDELAEELANLKVRLQHLRSIAEIDETLEQIRVKQHV
                     LERVRALREQNPMLGTRGVRLGIQLSELTRMQVRAIFEAAVACTREGIVVQPQVMIPL
                     VGHVNELRIQREVLEREVKQVLAENGNIRIPYTFGTMIEVPRAALTADEIATVAGFFS
                     FGTNDLTQMTFGISRDDAERGFMREYQARGILEDNPFATLDRNGVGRLMRLAVEAGRA
                     TNAQLHIGICGEHGGDPRSIAFCHEIGLDYVSCSPYRVPIARLAAAHAALGA"
     gene            complement(3149..3907)
                     /locus_tag="PLW13_06010"
     CDS             complement(3149..3907)
                     /locus_tag="PLW13_06010"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY69962.1"
                     /translation="MSFISIRHLWKEYGRRVVLENISLDFERGEFVSIVGASGCGKTT
                     FFKMLLGEERPTRGTMLLDGKSWPAEPGADRGVVFQRYSVFPHLTALQNVMPGLECRD
                     AMLSARLFGARRRAALERSRAMLEAVGLGDSGAKYPHELSGGMQQRLALAQSLIREPE
                     ILLLDEPFGALDPGIRADMHELLLSLWERKPFSVFMVTHDIQEGFTLGTRLLVFDRVR
                     HDPQEPDAYGARVSYDLPLKPASKRGGAGARAAA"
     gene            complement(3904..4737)
                     /locus_tag="PLW13_06015"
     CDS             complement(3904..4737)
                     /locus_tag="PLW13_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY69963.1"
                     /translation="MKRAINIRPTRLHALMLALLPFVLLAGIYVSASLARHATNPNDR
                     LLPSVSQLADGVRRVAFQEDKRTGRIILWSDTRASLQRLAWGVGISTASGLVFGMMLG
                     MFPLVRSTFAPTVAVLSLIPPMAVLPIIFVMCGLGEVSKVVLIVLGIAPVIIRDMQGR
                     VQDLPREQLIKAQSLGASSWQIALRVVLPQVLPRLLDALRLSLGAAWLFLISAEAIAA
                     EDGLGYRIFLLRRYLAMDVILPYVAWITLLAWLMDRALRELHRKAFPWLHANTATGEA
                     P"
     assembly_gap    4762..4861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5237..6268
                     /locus_tag="PLW13_06020"
     CDS             5237..6268
                     /locus_tag="PLW13_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQY69964.1"
                     /translation="MSSWCPALLYPGLFRHRDGVAPNLLAFAYAFGGYAAGWWLITRG
                     AIAPAVAGTLLLAHAMVIAAYLVHECAHNTLFRDNAFNARAGAALLWLCGACYGSYEG
                     LRHKHFRHHVDRADVVAFNFRTRLLRHPLLLRALQALEWLYIPAVDLLMHWLVPVLPF
                     VMPTRKAQRPRVVAILLVRGILFAAAGLLAPAALACYAIAYLLMLHLLRFMDAYQHTY
                     PLLETLEQAPGAQAKQFDAAFEASHTFSNPHSLRWPWLNLLTLNFGFHNAHHEKPNQP
                     WHRLPALHRSLFGAGLAQLIPLANQLACYHRYRVRRVVHEDPLDLDVTGNRGATFVGV
                     DGVSFLTGH"
     gene            6294..7076
                     /locus_tag="PLW13_06025"
     CDS             6294..7076
                     /locus_tag="PLW13_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HQY69965.1"
                     /translation="MTAVTRFWPLLCGTQRYEKTLSTRGTGAGVIIEAPILAYLVETA
                     NGRILYDVGCDYRKLSEPALRAQWYGNPAFPFGPPEVTEADRLPARLARLGLDVRDID
                     CVVCGHLHFDHGGGLHEFAHAEVHVHRAELQAMREQADEAYFRDDFAHGAHWCEHAGE
                     ATLAAGVQLIETPGHTAGHMSLLIELPEGKPVLLCGDAADLQENLDAEIAPGLCWRER
                     TDLALASIRKLKRIGAESGAELWPNHDMAFFRARDTFPVARR"
     gene            7073..7942
                     /locus_tag="PLW13_06030"
     CDS             7073..7942
                     /locus_tag="PLW13_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY69966.1"
                     /translation="MSGAAVSQRLPVLALLGASMIWGLTWLPLKLFEAGGLGGAALLL
                     VAHGTVALVALPLLARRCRALLPEWRTLAGVFCLGGFATIAFSSSLMYGDVVRAMSLF
                     YTMPLWAILGGLLFLGERLTRRRALAALCAITGAWLILGGTRILENPPGWIDLLAAAS
                     GFAYAMNNVVFRATPALAVAPKILMMYLGVAVFCALLLLSGVQQWPVEPPPASLLALA
                     CAFGACWLLAGNIGTQWGVTHMEAGRAAMIVVLELLIAVVSALWLEGGSLSAGEWCGA
                     ALLISAALLEARG"
     gene            complement(8060..9151)
                     /locus_tag="PLW13_06035"
     CDS             complement(8060..9151)
                     /locus_tag="PLW13_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY69967.1"
                     /translation="MASRIGVLYTRARRAAGGLLLATTLLGVAAPTLAAEKRSFTLAW
                     SIYAGWMPWPYAEQSGILKKWADKYGITIGLKQLDYIEGINQYTAGQFDAVLVTNMDA
                     LTIPAAGGVDTSIVIVGDYSNGNDGLVLKGADKLAAIKGQKVNLVELSVSHYLLARAL
                     DSVGLSEKDIQVVNTSDADIVAAWSTPDVTAAAAWNPQLAEMAKQAGTHLVYDSSQIP
                     GEILDALAINTATLEANPELAKALTGAWFETVALMSKGDAAAAAARTQMAQLSGTDLA
                     GYESQLKTTKLFYTPQEALAFATAPALLASNDLVRKFSFDHGLLGEGAPDADHIGIEF
                     PGARVLGDPKNIKLRFDARFVQMAAEGKL"
     regulatory      9457..9554
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            9609..10334
                     /locus_tag="PLW13_06040"
     CDS             9609..10334
                     /locus_tag="PLW13_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HQY69968.1"
                     /translation="MTLHDTPSPAVVEELLPGGGHRSFVLRRGQLLRLTDLEGGANVA
                     TLMFNALEKSERLNLPDSLKCQHTAKLTRGHCLYSDMGRVLAAITADTAGWSDSIGGV
                     LDAAEVREKYGAGSFQQLRNDCHRNGTDNLLIELGKWGLGLSDLLMVLNLFSRVDVEA
                     DGGLRFVPGNSRAGDFIELYAPMDTLVVLSALQHPLDPAPRYAPKPVQLAWLRAGPEV
                     AQHCRGSCAENARGFLNTERLYL"
     gene            10345..10995
                     /locus_tag="PLW13_06045"
     CDS             10345..10995
                     /locus_tag="PLW13_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HQY69969.1"
                     /translation="MSTSTATLTSSTREAADACLRHVIPAGEPWVGELTAGQTIRLLD
                     LEGNQAVDTLFFARANPRERYDPQRTLRRQGNLYLSTGTVLYSNLGRALLTIVADTCG
                     RHDTLGGACAQESNTVRYALEKRHMHSCRDSFLCGCLHDGRLDKRDIGANINFFMNVP
                     VTPAGGLTFEDGISAPGKYVELRAETDVIVLISNCPQLNNPCNAYDPTPVEVLVWN"
     gene            10999..>11377
                     /locus_tag="PLW13_06050"
     CDS             10999..>11377
                     /locus_tag="PLW13_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQY69970.1"
                     /translation="MFDKVLIANRGAIACRIARTLRELGAGAVAVYARADAASRHVTD
                     ADEAWSLGEGAAATTYLDAERILAIAREAGAAAIHPGYGFLSENAAFAERCAAAGIAF
                     VGPTPEQLRVFGLKHTARALAKAH"
     assembly_gap    11378..11477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11478..14762
                     /gene="uca"
                     /locus_tag="PLW13_06055"
     CDS             <11478..14762
                     /gene="uca"
                     /locus_tag="PLW13_06055"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HQY69971.1"
                     /translation="QLRVFGLKHTARALAKAHGVPLLEGTELLESVEAALAAAARVGY
                     PVMLKSTAGGGGIGMRVCRDDEELARQFDTVRRLGENNFSDAGVFLEKYIERARHLEV
                     QIFGDGQGEVIALGVRDCSVQRRNQKVLEETPAPNLPAGVAEALCAAAVRLGRAVSYR
                     SAGTVEFVYDSASGAFNFLEVNTRLQVEHGVTEQVWGVDLVAWMLRLAAGELPPLAEL
                     AARLSPAGHAIQARVYAEDPARDFRPSPGLLTEVAFPPADGRRLRIDTWVEAGCEIPP
                     SFDPMIAKLIAHAPDREAARAALDRALAATRLYGVETNRDYLRQILADAPFAGGEPWT
                     RCLEGLACSSHTVEVLAAGTQSTVQDWPGRIGYWAVGVPPSGPMDDRALRLGNRLLGN
                     AEGETALEITMSGPTLRFCCAAVIAITGAAIPFSIDGAPQPMNTTVFVPALARVELGT
                     IAGAGARSYLCVRGGFDLPAYLGSRSTFTLGQFGGHGGRALRAGDVLRLHPLADAQAG
                     ARLPPTLVPEPGALRRLRVIYGPHGAPEYFTPRYIEQFLATDWEVHFNSSRTGVRLLG
                     PKPEWARESGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVCPVTVIE
                     ADLWQLGQLKAGDRLRFVPVDLASARALAAARRRECAQLESTAPDCTEAVSLASPVVM
                     STGEGDTRLVARLSGDTHLLLEIGPPELDLVLRFRAHALMQAIEAEDFAGLVDLTPGI
                     RSLQLHYVPETLPLAALLARIGALWTDVCARDNLEVPSRIVHLPLSWDDPACRLAIEK
                     YMTTVRKDAPWCPSNLEFIRRINEVDDIEAVRGIVFAAHYLVMGLGDVYLGAPVATPL
                     DPRHRLVTTKYNPARTWTAENSVGIGGSYLCIYGMEGPGGYQFVGRTLQMWNRYREVA
                     AFGGKPWLLRFFDQIRFFPVEAAELERIRREFPLGRYPVRIEQTVLRLGEYQEFLARE
                     AESIARFRQRQRHGFDAERERWIATGQAHFEEGEIAPDVGEDEALGHGEHAIDSHIAG
                     NLWQVCVEPGQAVSAGETLAILESMKMEIPLTAPRAGIVKDVRLRPGSAVRAGQRVLV
                     LEETGT"
     gene            14759..16573
                     /gene="atzF"
                     /locus_tag="PLW13_06060"
     CDS             14759..16573
                     /gene="atzF"
                     /locus_tag="PLW13_06060"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HQY69972.1"
                     /translation="MRQAAHEDDLRIASLQQAYRSGALRPRQLIARLRARAAALNPEF
                     RLFIRLLDEAELEPYLAALEARPAQELPLYGIPFAIKDNIDLAGVPTTAACPAFAYTP
                     QRSASVVERLVALGAIPLGKTNLDQFATGLNGTRSPWGACRNAVLAQYISGGSSAGSA
                     LAVALGVASFALGTDTAGSGRVPAALNNLVGAKPTLGLLSTAGVVPACRTLDCVTYFT
                     ASAREASELLALTAARDPRDPYSRQNPSWNHGGAFGAPRPLRFGVPRPADLEFFGCAE
                     GPTLFAGAIAHLEAIGGEAHEIDFAPFLQAARLLYEGPWVAERYAVAGELIERDPDAV
                     LPVIRDVLAAAPRASAVEAFRARYRLQELKARCDATLEGLDCVLTPTIGRPVTLAEMQ
                     AEPVLRNSELGYYTNFMNLLDYAAVAVPSGFMANGLPWGITLFGRAFTDQYLLGIADA
                     IQRHHNLPLAGNHAPMLAQPPQAAGNDRLRLVVCGAHLEGLALNWQLTARGARLLERT
                     ASSPHYRLYALAGGPPARPGMVRVASGGCSIAVEVWELPASELGTFVAGIAAPLGLGK
                     LELADGRRETGFICEPCGLEDARDISHFGGWRQYLASG"
     gene            16696..18069
                     /locus_tag="PLW13_06065"
     CDS             16696..18069
                     /locus_tag="PLW13_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY69973.1"
                     /translation="MSDTPVRTALPRQRMLAVLAVTLATATVSFDGGIANVALPTIAR
                     ELGVPGSSAVAIISTYQIVLVVTLLPLAALAERIGHRRMLRIGIVLFIAGGLLCTFAR
                     TLPVLIALRACQSLGSAAVMCVGAALIRSLYPPEWLGRGLALNTLIATASLSLAPTVG
                     GLMLAVQPWYWLFAIGVPTTLAALLLSGIVPESERRDTPFDVRGALYCMASFGLLFAG
                     IESAVHGAEPLLTALLIGLGVPVTVRFVQRELRASMPILPLDLLGRPMIALTVGGAML
                     TFIASTSLLVTLPFRLHEQFGFSTAATGAILAAWSLTMMFFAPSAGILSDRIPAPVLG
                     AFGMIVASVGALLLALLPEDANGFDISWRIAVCAAGYAFYVSPAFRMVVNAAPPAGVA
                     SAGSLMTTARMSGQTLGATASATLLVLGIGQGAAPAAIAALLFFSAGVLGIIRFSPGI
                     ATRSLPP"
BASE COUNT         2604 a         6445 c         6172 g         2759 t
ORIGIN      
        1 tccagaaacg cggcggcggt gggatagcgc gcgatgaacg cgatgtccca gcgctcctcg
       61 ggcggtccga tcacgacctg ctcgggccgc ccgcgccaca cgatgcgtcc gccgacccgc
      121 tggaacacgg ggccgctctc gcgcgagtag gcggcgtagg cggccttgcc gctgccgcca
      181 cgcccgtccg ggtacgcggc gtcggcgcgc aaccggatga ggttcagcat caggatcggg
      241 gtgtcgcgcg gcagcgcctt gaaggcggcg aaatgctcgc ggctcggatc gacgtggtgg
      301 gacatgctcg ttgctcctca ggcgcccagc gccgcatgcg ccgcggcaag ccgcgcgatg
      361 ggcacgcgat atggcgagca ggacacgtag tccagcccga tctcgtgaca gaaggcgatg
      421 ctcctcggat cgccgccgtg ctcgccgcag atgccgatgt gcagctgcgc gttggtggcg
      481 cggcccgcct ccacggcgag ccgcatcagg cgcccgacgc cgttgcgatc gagcgtcgcg
      541 aacgggttgt cctcgaggat gccgcgcgcc tggtattcgc gcatgaagcc gcgctcggcg
      601 tcgtcgcgcg agatgccgaa ggtcatctgc gtgaggtcgt tggtgccgaa ggagaagaac
      661 ccggccacgg tggcgatctc gtcggccgtg agcgcggcgc gcggcacctc gatcatggtc
      721 ccgaaggtgt agggaatgcg gatgttgccg ttctccgcca gcacctgctt cacctcgcgc
      781 tcgagcacct cgcgctggat gcgcagctcg ttcacgtgcc cgacgagcgg gatcatcacc
      841 tgcggctgca ccacgatgcc ctcgcgcgtg caggcaacgg ccgcctcgaa gatcgcgcgc
      901 acctgcatgc gggtcagctc gctcagctgg atgccgaggc gcacgccgcg cgtgcccagc
      961 atcgggttct gctcgcgcag cgcgcgcacg cgctccagca catgctgctt cacgcgtatc
     1021 tgctccagcg tctcgtcgat ctcggcgatg ctgcgcaaat gctgcagccg caccttgagg
     1081 ttcgccagct cctcggcgag ctcgtcgtag gcggggagga actcgtgcag cgggggatcg
     1141 agcaggcgga tggtcacggg cctgccctcc atcgcgcgaa acagcccctc gaagtccgag
     1201 cgctgtggcg gcagcagcgc cgcgatggcc tcgttgcgct ccgagggctc cctggccatg
     1261 atcatgcgct gcaccagcgg catgcgctcc gcctcgaaga acatgtgctc cgtgcggcac
     1321 aggccgatgc cctgcgcgcc gtagagccgg gcacgcgcgg cgtcgcgcgg gtagtccgcg
     1381 ttcgcccaca cctccaggcg gcggacctcg tcggcccatt cgagcagcct gatcaggtag
     1441 gtgttgctga gatcgggcac cagcgtgggc agcttgccga cgtagaccag gccattgctg
     1501 ccgtcgatgg aaatccagtc gccttccctg accaccacgt cgccaaggcg catctcgcgc
     1561 cgctcgaggt cgatttccag cgcctcgata cccaccacgg cgggcttgcc gaactggcgc
     1621 gccaccagcg ccgcatggct cgtgcgcccg ccgcgactgg tgaccacgcc cctggccgcc
     1681 agcatgccgt gcacgtcgtc gggcttcgtt tccgggcgca ccatgatgac gggcttgtcc
     1741 tcgtcgcgtc cccagcgctg cgcgagcgcg gcgtcgagcg ccaccatccc gaccgccgcg
     1801 cccggcgaca cgttgaggcc gctgccgatc agctcggccc tgcgcacggc atcgggctcg
     1861 agctgcgggt gcaggaagaa gtcgacctgt tccgggcgca cgcggcgcag cgcctcctcg
     1921 cgtgtgatca gcccctcctc gaccatgtcc acggcgatgc gcacctcggc ctgcgccgtg
     1981 cgcttggcat cgcgcgtctg cagcagccag agcctgccct gttcgatggt gaactccatg
     2041 tcctgcacgt tgcggtagtg catttccagc cgcgccgcga tgccggcgaa atcgcggtag
     2101 atctccggca tctgctgcgc gagctccgcg agcggctgcg tgaggcggat tcccgcgacc
     2161 acgtcctcgc cctgcgcatc gaacaggaaa tcgccctcga gctcgcgctc gccggtcgag
     2221 acgttgcgcg acatcgccac gcccgtggcg gagttcgcgc ccatgttgcc gaacaccatg
     2281 gtctggatgt tgaccgccgt gccgagatcg tgcgggatgc cggcggcgtt gcggtagtcc
     2341 accgcgcgct tgccgttcca cgagcggaac acggcctcgg tagccaggcg cagctgctcg
     2401 tagggatcgg aggggaaatc cgcgccggtg taggtgcggt agatctcctt gaagcgcgcg
     2461 gtgatcaggc gccagtgccc ggcatccatc ccggcatcgc tcgccacgcc gaggcgattg
     2521 cgctgcgccg tgatgaccgc atcgaagacc tcgtcgggca cgcccatcac gacgctgccg
     2581 aacatctgta cgagacgacg gtagagatcg aaggcaaagc gctcgtcgcc gctgcgtcgc
     2641 gccagcccgg ccaccacctc gtcgttcagg ccgatattga ggatcgtgtc catcatgccg
     2701 ggcatcgact gcctggcgcc ggagcggcag gacaccagca gggggttgtc ccgatcaccg
     2761 aagcgcttgt gcgtcgcgga ctcgatcgcg cgcacggcag cgagaacctc ctcccacagc
     2821 gtgccgggca gccgggcatc ggcggcgagg aagtcgcggc acgccgcggt gctgatcgtg
     2881 aagccgggcg gcaccgggat gccgagactg ctcatctcgg cgagattggc tcccttgccg
     2941 ccgagcagcg cccgcacggc ctgccagtcc ccgcccgcgc tggcgcgcac cgcgtcaatc
     3001 tcgctgaaca ggtagacgta gcgcgcgctg ctgtcggccg gcatggcagt tccccgcatc
     3061 gttgctggtg ggcgcatggt gcgcccgccg catcgcgcga gtccagttca tcgtcgcgcg
     3121 cgcgaggaca cggtcgcgcg ctgggttgct aggcggcggc tcgagccccg gcgccgccgc
     3181 gcttcgacgc gggcttcagc ggcaggtcgt aggacacccg cgcaccatag gcgtcgggct
     3241 cctgggggtc gtggcgcacg cggtcgaaca ccagcaggcg cgtgcccagc gtgaagccct
     3301 cctggatgtc gtgcgtcacc atgaacacgc tgaagggctt gcgctcccac agcgacagca
     3361 gcagctcgtg catgtcggcg cggatgcccg gatccagcgc cccgaagggc tcgtcgagca
     3421 gcaggatctc gggctcgcgg atcagcgact gcgccagtgc caggcgctgc tgcatgccgc
     3481 cggagagctc gtgcgggtac ttcgcgccgc tgtcacccag gccgacggcc tccagcatgg
     3541 cccgcgagcg ctccagcgcg gcgcggcgcc tggcgccgaa gaggcgcgcg ctcagcatcg
     3601 cgtcgcggca ttccaggccg ggcatcacgt tctgcagcgc cgtgaggtgc gggaacaccg
     3661 agtagcgctg gaacaccacg ccgcgatcgg cgccgggctc cgcgggccag gacttgccat
     3721 cgagcagcat cgtgccgcgg gtgggcctct cctcgccgag cagcatcttg aagaacgtgg
     3781 tcttgccgca gcccgaggcg ccgacgatgc tgacgaactc gccgcgctcg aaatcgagcg
     3841 agatgttctc cagcacgacg cgccggccgt actccttcca caggtggcgg atgctgatga
     3901 agctcatggc gcctctccgg tggcggtgtt cgcgtgcagc caggggaagg ccttgcgatg
     3961 cagctcgcgc agggcgcggt ccatcagcca ggcgagcagc gtgatccacg ccacgtaggg
     4021 caggatcacg tccatcgcaa ggtaacggcg cagcaggaag atgcggtagc cgagcccgtc
     4081 ctcggcggca atggcctcgg cggagatcag gaacagccac gccgcgccga gcgacagccg
     4141 cagcgcatcg agcaggcgcg gcagcacctg cggcagcacc acgcgcagcg cgatctgcca
     4201 gctcgaggcg cccagcgact gcgccttgat cagctgctcg cgcggcaggt cctgcacgcg
     4261 cccctgcatg tcgcggatga tcaccggcgc gatgccgagc acgatcagca ccaccttgga
     4321 gacctcgccg aggccgcaca tcacgaagat gatcggcagc accgccatcg ggggaatcag
     4381 cgacagcacc gcgacggtgg gcgcgaaggt gctgcgcacc agcgggaaca tcccgagcat
     4441 catcccgaac accagcccgg acgcggtgct gatccccacg ccccaggcga ggcgctgcag
     4501 gctggcacgg gtgtcgctcc acaggatgat ccgcccggtg cgcttgtcct cctggaacgc
     4561 gacgcgccgc acgccgtcgg cgagctggct cacgctgggc agcaggcggt cattggggtt
     4621 cgtggcatgg cgtgccagcg aggccgacac gtagatcccg gcgagcagca cgaacggcag
     4681 cagggcgagc atgagcgcgt gcaaccgcgt cggccggatg ttgatcgctc gtttcatggt
     4741 cggatgccgg atccctggtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 ngcgtcggcc ggatgttgat cgctcgtttc atggtcggat gccggatccc tggtcgcctg
     4921 ttgcaaggcc aaatgcacgg aacatgccaa ccatcgagag ccgctgtgcg cccgaatgcc
     4981 cgccagttcg cacgagcagc gcgccgttgc ggtgcaatca cgcccgtgac gcaccgcaat
     5041 cgcgcatggc atgtccgcag cacgccggat aaggccgcct cgccccgcgt gccgcggcac
     5101 tggcgtgaat gttgctcgac cattattcga ttgcgactcg acaggctgca gtccaacgca
     5161 gaatgcttcc tgaagacccg ttgccggacg gccggcaatg cgtagacccc gtgggacggc
     5221 ccaccatgga aacccgatgt cgtcctggtg ccctgcactc ctgtatcccg gattgttccg
     5281 ccaccgcgat ggcgtggcgc ccaacctgct cgccttcgcc tacgcgttcg gcggctacgc
     5341 cgccgggtgg tggctgatca cgcgcggcgc gatcgcgccc gccgttgccg gcaccctgtt
     5401 gctggcgcac gcgatggtca tcgcggcgta cctggtgcat gaatgcgcgc acaacaccct
     5461 gttccgcgac aacgccttca atgcgcgcgc gggcgcggcc ttgctgtggc tctgcggcgc
     5521 ctgctacggc agctacgagg gcctgcgcca caagcacttt cgccaccacg tggaccgcgc
     5581 cgacgtggtg gcattcaact tccgcacgcg cttgttgcgc cacccgttgc tgctgcgcgc
     5641 gctgcaggcg ctggagtggc tgtacatccc cgccgtcgac ctgctcatgc actggctggt
     5701 gccggtgctt cccttcgtga tgcccacgcg caaggcccag cggccccgcg tcgtcgctat
     5761 cctgctggtg cgcggcatcc tgttcgcggc tgcgggcctc ctcgcgcccg cggccctcgc
     5821 ctgctacgcg atcgcctacc tgctgatgct gcacctgctg cgcttcatgg acgcgtacca
     5881 gcacacctac ccgctgctcg agaccctgga gcaggcgccg ggcgcgcagg caaagcagtt
     5941 cgacgcggcc ttcgaggcga gccacacctt cagcaacccg cattcgctgc gctggccgtg
     6001 gctgaacctg ctgacgctca acttcggctt ccacaacgcg caccacgaga agccgaacca
     6061 gccctggcac cgcctgcccg cgctgcatcg cagcctgttc ggcgccgggc tggcgcagct
     6121 gatcccgctg gcaaaccagc tggcctgcta ccaccgctac cgcgtgcggc gcgtggtgca
     6181 cgaggacccg ctcgatctgg acgtgaccgg caaccgtggc gccaccttcg tgggcgtcga
     6241 tggcgtctcc ttcctgaccg ggcactgagc attcatcacc ggagcaccac gccttgaccg
     6301 ccgtcacccg tttctggccg ctgctgtgcg gcacccagcg ctacgagaag accctgtcga
     6361 cgcgcggcac cggcgccggg gtgatcatcg aggcgccgat cctcgcctac ctggtcgaga
     6421 ccgccaacgg ccgcatcctc tacgacgtgg gctgcgatta ccgcaagctg agcgaaccgg
     6481 cgctgcgcgc gcagtggtac gggaatcccg ccttcccctt cggcccgccc gaggtcaccg
     6541 aggccgaccg gctcccggca cggctggcgc gcctcgggct cgatgtccgc gacatcgact
     6601 gcgtggtgtg cggtcacctg cacttcgatc acggcggcgg gctgcacgag ttcgcgcacg
     6661 ccgaggtgca cgtgcatcgc gccgagctcc aggccatgcg cgagcaggcg gacgaggcct
     6721 actttcgcga cgacttcgcg cacggggcgc actggtgcga acacgcgggc gaagcgacac
     6781 tcgcggcggg cgtgcaactc atcgagacgc cgggacacac cgcgggccac atgtcgctgc
     6841 tgatcgagct gccagaaggc aagcccgtgc tgctgtgcgg cgacgcggcc gacctgcagg
     6901 agaacctcga cgcggaaatc gccccggggc tgtgctggcg cgagcgcacc gatctggcac
     6961 tcgcgagcat ccgcaagctc aagcgcatcg gcgcggaatc gggcgcggag ctctggccca
     7021 accacgacat ggcgttcttt cgcgcccgcg acacctttcc ggtcgcgcgg cgatgagcgg
     7081 cgccgcggtc tcgcagcgcc tgcccgtgct ggcgctgctc ggcgcctcga tgatctgggg
     7141 cctgacctgg ctgccgctca agctgttcga ggccggcgga ctgggcgggg cggcactgct
     7201 gctggtcgcc cacggcacgg tggcactggt ggcactgccg ctgctcgcgc gccggtgccg
     7261 cgcactgctg cccgagtggc gcacgctggc aggtgttttc tgcctcggcg gcttcgccac
     7321 catcgccttc agctccagcc tgatgtacgg cgacgtggtg cgggcgatgt cgctgttcta
     7381 caccatgccc ctgtgggcca tcctcggcgg tctgctgttc ctgggcgagc gcctgacgcg
     7441 acggcgcgcc ctggccgcgc tctgcgccat taccggcgcg tggctgatcc tcggcggcac
     7501 gcgcatcctc gagaatccgc ccgggtggat cgacctgctg gcggcagcct cgggcttcgc
     7561 ctacgcgatg aacaacgtgg tcttccgcgc cacccccgcg ctggcggtgg cgccgaagat
     7621 cctcatgatg tacctcggcg tcgcggtgtt ctgcgcgctg ctgctgctct cgggcgtaca
     7681 gcaatggccc gtggagccgc cgccggcgtc gctgctggcg ctggcctgcg ccttcggcgc
     7741 ctgctggctg ctggccggca acatcggcac gcaatggggc gtcacacaca tggaggccgg
     7801 acgcgcggcg atgatcgtgg tgctggaact gctgatcgcg gtggtctcgg cgctgtggct
     7861 cgagggcggc tcgctgtcgg cgggcgagtg gtgtggcgcg gcgctgctga tcagcgccgc
     7921 gctgctggag gctcgcggct gatgtgcgac acctgccccc tgcagcaaca cgatgttgtg
     7981 cgcgacagca atgcacgcct gcatgaaaaa aaagccgccc gaaggcggcc agggagggtc
     8041 gaacgcgccg taatcggtgt tacagctttc cttctgcggc catctgcacg aagcgcgcat
     8101 cgaagcgcag cttgatgttc ttcgggtcgc cgagcacccg ggcacccggg aactcgatgc
     8161 cgatgtgatc ggcatcggga gcaccctcgc cgagcagacc gtggtcgaac gagaacttgc
     8221 gcaccaggtc gttcgaggcg agcagcgcgg gcgccgtcgc gaaggcgagc gcctcctgcg
     8281 gcgtgtagaa cagcttcgtc gtcttcagct gcgactcgta gccggccagg tcggtgcccg
     8341 agagctgcgc catctgcgtc ctggctgccg cggccgccgc gtcacccttg ctcatcagcg
     8401 ccaccgtttc gaaccaggca ccggtgagcg ccttggccaa ctccgggttg gcctcgagcg
     8461 tcgcggtgtt gatcgcgagc gcgtcgagga tctcgccggg aatctgcgag gagtcgtaga
     8521 ccaggtgcgt gcctgcctgc ttcgccatct cggccagctg cgggttccac gccgcggcgg
     8581 cggtgacatc gggagtgctc cacgcggcga cgatgtctgc gtccgaggtg ttgaccacct
     8641 ggatgtcctt ctcggagagg cccacgctgt ccagcgcgcg cgccagcagg tagtgcgaca
     8701 cggacagctc caccaggttc accttctgtc ccttgatagc ggcgagcttg tccgcgccct
     8761 tcagcaccag cccgtcatta ccgttggagt agtcgcccac gatcacgatc gaggtgtcga
     8821 caccgccggc ggcgggaatc gtgagcgcat ccatgttggt gaccagcacc gcgtcgaact
     8881 gcccggccgt gtactggttt attccctcga tgtagtcgag ctgcttgagc cctatcgtga
     8941 tgccgtactt gtcggcccac ttcttcagga tcccggactg ctcggcgtag ggccagggca
     9001 tccagccggc gtagatgctc caggcgagcg tgaagctcct cttctcggcc gccagcgtcg
     9061 gggcagcgac gccgagcagg gtggtggcga gcagcagccc gcccgcggcg cgccgtgcgc
     9121 gcgtgtacaa gactccgatt cgtgatgcca tggtcgatgt ccccggtgtg cgcttcgatg
     9181 aaatgccgtc acaccggctg agcaagaagc ctgccagctg gccacgaagc tcggctgcgg
     9241 aggcctggta ccgcgtaccg gcactgcttc ggctcggctc ggcgcacaat ggtggtgcgt
     9301 ggcgcgcggc gatggtgcat cctgtcggcg caacgggaga ccgttgcgcc atgcaggcgc
     9361 ggtgacaccg cgaacgcccg cattggcatg aatctggctt aatcaattgt cgaacgagag
     9421 cggtccggtg cgccggacgc cacgcaccac aatccggaaa tggatgacta gggttccggc
     9481 ctgcacgcag gtgactggtc cgagagtcat cgacctcctt cgaggttaca cggcgggaca
     9541 aaagcccggg agaacgcacg ctgtgttgcg cgctcccggc tgttccgtcg cccgaaccgg
     9601 aggttcacat gacattgcac gacaccccgt cacccgccgt agtcgaggag ttgctgcccg
     9661 gcggcggcca ccgctccttc gtgctgcgcc gcggccagct gctgcgcctg accgatctcg
     9721 agggcggtgc caacgtcgcc acgctgatgt tcaacgccct cgagaaatcc gagcgcctca
     9781 acctgcccga ttcgctgaag tgccagcaca ccgcgaagct cacgcgtggc cactgcctct
     9841 attccgacat gggccgcgtt ctggccgcga tcaccgccga caccgcgggc tggagcgaca
     9901 gcatcggcgg cgtgctggat gccgcggagg tgcgcgaaaa gtacggcgcg gggtcgttcc
     9961 agcagctgcg caacgactgc caccgcaacg gcaccgacaa cctgctgatc gagctcggca
    10021 agtgggggct ggggctgtcg gacctgctga tggtactgaa cctgttctcg cgcgtggacg
    10081 tggaggccga cggcgggctt cgtttcgtgc ccggcaattc gcgcgccggg gatttcatcg
    10141 agctctacgc gccgatggac acgctggtgg tgctcagcgc gctgcagcat ccgctggatc
    10201 ccgcgccgcg ctacgcgccg aagccggtgc agctcgcctg gctgcgcgcc ggccccgagg
    10261 tcgcgcagca ttgccgcggc tcctgcgccg agaacgcgcg cggtttcctc aacaccgaac
    10321 gcctttacct gtgaggatgc gaccatgagc accagcaccg cgacactgac gagcagcacg
    10381 cgcgaggcgg ccgatgcctg cctgcgccac gtcatcccgg cgggcgagcc ctgggtgggc
    10441 gagctcacgg cgggacagac gatccgcctg ctcgacctcg agggcaacca ggccgtcgac
    10501 acgctgttct tcgcgcgcgc caacccgcgc gagcgctacg acccgcagcg cacgctgcgc
    10561 cgccagggca acctctacct cagcaccggc accgtgctgt actcgaacct gggccgagcg
    10621 ctgctcacga tcgtcgccga cacctgcggg cggcacgaca cgctcggcgg cgcctgcgcg
    10681 caggagagca acaccgtgcg ctacgcgctg gagaagcgcc acatgcacag ctgccgcgac
    10741 agcttcctgt gcggctgcct gcacgacggg cgtctcgaca agcgcgacat cggcgccaac
    10801 atcaacttct tcatgaacgt gccggtgacg cccgcgggcg ggctcacgtt cgaggacggc
    10861 atctcggcgc ccggcaagta cgtcgagctg cgcgccgaga ccgacgtgat cgtgctgatc
    10921 tccaactgcc cgcagctgaa caacccctgc aatgcctacg acccgacgcc ggtcgaagtg
    10981 ctggtgtgga actgagccat gttcgacaag gtactgatcg ccaaccgcgg cgccatcgcc
    11041 tgccgcatcg cgcgcacgct gcgcgagctc ggcgccggcg ccgtcgccgt ctacgcgcgg
    11101 gccgacgccg ccagccgcca tgtcaccgac gccgacgagg cctggagcct cggcgagggt
    11161 gcggccgcca cgacctacct cgacgccgag cgcatcctcg cgatcgcgcg cgaggcgggc
    11221 gccgcggcca tccatcccgg ctacggcttc ctgtcggaga acgcggcctt cgccgagcgc
    11281 tgcgccgccg cgggcatcgc cttcgtcgga cccacgccgg agcagctgcg cgtgttcggc
    11341 ctcaagcaca ccgcgcgcgc gctggcgaag gcccacgnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnncag ctgcgcgtgt tcggcctcaa gcacaccgcg cgcgcgctgg
    11521 cgaaggccca cggcgtgccg ctgctcgaag gcacggaact gctggaatcc gtggaagcgg
    11581 cactcgccgc ggccgcgcgc gtcggttacc cggtgatgct gaagagcacc gcgggcggcg
    11641 gcggcatcgg gatgcgcgtg tgccgcgacg acgaggagct cgcgcgccag ttcgacaccg
    11701 tgcgccgcct cggcgagaac aacttcagcg acgccggcgt gttcctcgag aaatacatcg
    11761 agcgcgcgcg ccacctcgag gtgcagatct tcggcgacgg ccagggcgag gtgatcgcgc
    11821 tcggcgtgcg cgactgctcg gtgcagcggc gcaaccagaa ggtgctggag gaaaccccgg
    11881 caccgaacct gcccgccggc gtggccgagg cactgtgcgc ggcggcggtg cgcctcggac
    11941 gcgcggtgtc ctaccgttcc gcgggtaccg tcgaattcgt ctatgacagc gccagcggcg
    12001 cgttcaattt cctcgaggtg aacacgcggc tgcaggtcga gcacggcgtc accgagcagg
    12061 tgtggggcgt ggacctggtc gcgtggatgc tgcgcctggc cgcgggcgag ctgccgccgc
    12121 tcgcggaact ggcggcgagg ctctcacccg ccggtcacgc gatccaggcg cgcgtgtacg
    12181 ccgaggaccc ggcgcgcgat ttccgcccca gccccggcct gctgaccgag gtcgcgttcc
    12241 cgccggccga cggcaggcgg cttcgcatcg acacctgggt cgaggccggc tgcgagatac
    12301 cgccgagctt cgacccgatg atcgccaagc tcatcgcgca cgcgccggat cgcgaggcgg
    12361 cgcgcgcagc gctcgaccgg gcgctcgccg cgacacggct ctacggcgtc gagaccaacc
    12421 gcgactacct gcgccagatc ctcgccgacg cgcccttcgc cggcggcgag ccgtggactc
    12481 gctgcctcga gggactggcc tgcagcagcc acaccgtcga ggtcctcgcc gccggcacgc
    12541 agagcacggt gcaggactgg cccggacgca tcggctactg ggccgtcggc gtgccgccgt
    12601 cggggccgat ggacgatcgc gcgctgcgtc tgggcaaccg cctgctcggc aatgccgagg
    12661 gcgagaccgc gctggaaatc acgatgagcg gtccgacgct gcgcttctgc tgcgccgcgg
    12721 tgatcgcgat caccggtgcc gcgatcccgt tcagcatcga tggtgcgccg cagccaatga
    12781 acaccacggt gttcgtgccc gcgctcgcgc gcgtcgaact cggcacgatc gcgggcgccg
    12841 gcgcgcgcag ctacctctgc gtgcgcggcg gcttcgacct gccggcctat ctcggcagcc
    12901 gcagcacctt cacgctgggg cagttcggcg gccacggcgg ccgcgcgctg cgcgccgggg
    12961 acgtgctgcg cctgcacccg ctcgccgacg cgcaggccgg cgcgcggctg ccgccgacgc
    13021 tggtgcccga gcccggtgcg ctgcgccggc tgcgcgtgat ctacggcccg cacggcgcgc
    13081 ccgaatactt cacgccacgc tacatcgagc agttcctcgc caccgattgg gaagtgcact
    13141 tcaattccag ccgcaccggc gtgcgcctgc tgggacccaa gcccgaatgg gcgcgcgaga
    13201 gcggcggcga ggcggggctg catccctcca acatccacga caacccctat gcggtcggcg
    13261 ccgtcgactt caccggcgac atgccggtga tcctcggtcc cgacggtccc agcctcggcg
    13321 gcttcgtctg ccccgtgacc gtgatcgagg ccgacctgtg gcagctcggc cagctcaagg
    13381 ccggcgaccg gctgcgcttc gtgccagtcg atcttgccag cgcgcgcgcg ctcgccgccg
    13441 cgcgccggcg cgaatgcgcg cagctcgaga gcaccgcacc ggactgcacc gaggcggtat
    13501 cgctcgcttc gccggtggtg atgagcaccg gtgaaggcga cacgcggctg gtcgcgcggc
    13561 tctccggcga cacgcacctg ctgctcgaga tcgggccgcc ggaactcgac ctggtgctgc
    13621 gctttcgcgc ccacgcgctg atgcaggcca tcgaggccga ggacttcgcg ggcctcgtgg
    13681 acctcacgcc cggcatccgc tcgctgcagc tgcactatgt tccggaaacg ctgccgctgg
    13741 ccgcgctgct ggcgcgcatc ggcgcgctgt ggaccgatgt ctgcgcccgc gacaatctcg
    13801 aggtgccctc gcgcatcgtg cacctgccgc tgtcgtggga cgatccggcc tgccggctcg
    13861 cgatagagaa atacatgacc acggtgcgca aggacgcgcc gtggtgcccg agcaatctcg
    13921 agttcatccg ccgcatcaac gaggtcgatg acatcgaagc ggtgcgcggg atcgtcttcg
    13981 ccgcgcacta cctggtgatg ggcctcggcg acgtgtacct cggcgcgccg gtcgccacgc
    14041 cactggatcc gcgccaccgc ctggtcacca ccaagtacaa cccggcgcgc acgtggaccg
    14101 cggagaactc ggtgggcatc ggcggctcgt atctttgcat ctacggcatg gaaggcccgg
    14161 gcggctacca gttcgtcggg cgcacgctgc agatgtggaa ccgctaccgc gaggtggcgg
    14221 ccttcggcgg caagccgtgg ttgctgcgct tcttcgacca gatccgcttc ttcccggtcg
    14281 aagccgcaga gctcgagcgc atccgccgcg agttcccgct ggggcgttac ccggtccgca
    14341 tcgagcagac cgtgctgcgc ctgggcgagt accaggaatt cctggcgcgc gaagccgaat
    14401 cgatagcgcg tttccgccag cgccagcgcc acggcttcga cgccgagcgc gagcgctgga
    14461 tcgccaccgg gcaggcgcat ttcgaggagg gcgagatcgc ccccgacgtg ggcgaggacg
    14521 aggcgctggg ccacggcgag cacgccatcg acagccacat cgccggcaac ctgtggcagg
    14581 tctgcgtcga gcccgggcaa gcggtgagcg ccggcgagac gctggcgata ctcgaatcga
    14641 tgaaaatgga gatcccgctc accgcgccac gcgcgggcat cgtgaaggac gtgcgcctgc
    14701 gaccgggatc ggccgtgcgc gccggccagc gcgtgctggt actggaggag acaggaacat
    14761 gaggcaagcc gcgcacgagg acgacctgcg catcgcgtcg ctgcagcaag cctaccgcag
    14821 cggcgcgctg cgtccgcgcc agctgatcgc gcggctgcgc gcgcgcgccg cggcgctgaa
    14881 cccggagttc cgcctgttca tccggctgct ggacgaagcg gagctggagc cgtacctggc
    14941 agcgctggag gcacgcccgg cgcaggaact gccgctctac ggcatcccgt tcgcgatcaa
    15001 ggacaacatc gacctcgccg gcgtgcccac gacggcggcc tgccccgcct tcgcgtacac
    15061 gccccaacgc tcggccagcg tcgtcgagcg cctggtcgcg ctcggcgcca tcccgctcgg
    15121 caagaccaat ctcgaccagt tcgccaccgg tctcaacggc acgcgctcgc cctggggcgc
    15181 ctgccgcaac gccgtgctgg cgcaatacat ctcgggcggc tcgagcgcgg gctccgcgct
    15241 cgccgtcgcg ctcggcgtgg ccagcttcgc gctcggcacc gacaccgcgg gatccgggcg
    15301 cgtgccggcg gcgctgaaca acctcgtcgg cgccaagccc acgctcgggc tgttgtcgac
    15361 ggccggcgtg gtgcccgcct gccgcacgct cgactgcgtg acgtatttca ccgccagcgc
    15421 gcgcgaggcc tcggagctgc tggcattgac cgcggcgcgc gatccgcgcg atccctacag
    15481 ccgccaaaac ccgtcgtgga accacggcgg cgccttcggt gcgccgcgcc ccttgcgctt
    15541 cggtgtgccg cggcccgcgg atctcgagtt cttcggctgc gccgagggac caacgctgtt
    15601 cgccggggcg atcgcgcatc tggaggcgat cggcggcgaa gcccacgaga tcgacttcgc
    15661 gccgttcctg caggccgcgc gcctgctcta cgaaggcccg tgggtcgccg agcgctacgc
    15721 ggttgccggc gagctgatcg aacgcgaccc cgacgcggtg ttgccggtga tccgtgacgt
    15781 gctcgcggcc gcaccgcggg caagcgcggt cgaggctttc cgtgcccgct accggctgca
    15841 ggaattgaag gcacggtgcg acgccacgct cgaggggctg gactgcgtgc tcacgccgac
    15901 catcggccgg ccggtcacgc tcgccgaaat gcaggccgag ccggtgctgc gcaacagcga
    15961 gctcggctac tacacgaact tcatgaacct gctcgattac gcggccgtcg cggtaccgag
    16021 cggcttcatg gcaaacggcc tgccgtgggg catcacgctg ttcggtcgcg ccttcaccga
    16081 ccagtacctg ctcggcatcg ccgacgccat ccagcgccac cacaatctcc cgctcgcggg
    16141 caaccacgcg ccgatgctgg cgcaaccgcc gcaggcggcc gggaacgatc gcctgcgcct
    16201 ggtggtttgc ggtgcgcacc tcgagggcct ggcgctgaac tggcagctca cggcgcgcgg
    16261 ggcgcgcctg ctggagcgca ccgcgagcag cccccattac cggctgtacg cgctggccgg
    16321 ggggccgccc gcgcgcccgg gcatggtgcg cgtcgccagc ggcggctgca gcatcgcggt
    16381 ggaggtgtgg gaactgccgg ccagcgagct gggcacattc gtcgccggca ttgcggcgcc
    16441 actgggactc ggcaagctcg aactcgccga cgggcgccgc gagaccggtt tcatctgcga
    16501 gccctgcggc ctcgaggacg ccagggacat cagccacttc ggcggctggc gacagtacct
    16561 cgccagtggc tgagcgcacc ggcgcggggg ccgtcgcgac ggccacggcg caagcagtgg
    16621 cccgccggcc cggttacact gcgccgggat cccgcgtgcg cgggccgaac gatacgcggg
    16681 gagggaaagg cgcgcatgag cgatacgccg gtccgcacgg cgctgccgcg acagcggatg
    16741 ttggccgtgc tggctgtgac gctggcgaca gcgacggtat cgttcgacgg cggcatcgcc
    16801 aacgtcgcgc tgccgacgat cgcgcgcgag ctcggcgttc cgggctcgag cgcggttgcg
    16861 atcatctcca cctaccagat cgtcctcgtg gtcacgctgc ttccccttgc ggcactcgcc
    16921 gagcgtatcg gtcaccggcg catgttgcgc atcggcatcg tcctgttcat cgccggagga
    16981 ctgctctgca ccttcgcgcg cacgctgccg gtcctgatcg cgctgcgcgc atgccagtcg
    17041 ctgggctcgg cggcggtgat gtgcgtcggc gcggcgctga tccgctcgct ctatccgccc
    17101 gaatggctcg gacgcgggct cgcgctcaac acgctgatcg cgaccgcgtc gttgtcgctc
    17161 gcaccgaccg tcggcggact gatgctcgcg gtccagccct ggtactggct cttcgccatc
    17221 ggcgtgccca cgaccctggc ggcgctgctg ctgtcgggca tcgtcccgga aagcgagcgg
    17281 cgcgacacgc ccttcgacgt tcgcggcgcg ctctattgca tggcgagctt cggcctcctg
    17341 ttcgcgggga tcgagagcgc ggtgcacggt gccgagcccc tgctcacggc gctgctgatc
    17401 gggctcggcg tgccggtgac cgtgcgcttc gtgcagcgcg agctgcgcgc gagcatgccg
    17461 atcctgccgc tcgacctgct cgggcggccc atgatcgcgc tgacggtggg cggggcgatg
    17521 ctcacgttca tcgccagcac gagccttctc gtcaccctgc cgttccgtct gcacgagcag
    17581 ttcgggttca gcaccgcggc gaccggcgcg atactcgcgg cctggtcgct gacgatgatg
    17641 ttctttgcgc cgagcgccgg gatcctgtcc gaccgtatcc ccgccccggt gctcggcgcg
    17701 ttcggcatga tcgtggcaag cgtcggggcg ctgctgctcg ccctgcttcc cgaggacgcg
    17761 aacgggttcg acatcagctg gcggatcgcg gtctgcgcgg cgggctacgc cttctacgta
    17821 tcgcccgcct tccgcatggt ggtcaacgcc gcgccaccgg caggcgtggc ctcggcgggc
    17881 agcctcatga ccaccgcccg gatgagcggc cagacgctcg gcgcgaccgc ttcggcgacg
    17941 ctgctcgtgc tcgggatcgg ccagggcgcg gcaccggcgg cgatcgcggc gctgctgttc
    18001 ttcagcgcgg gtgtcctcgg gatcatccgc ttctcgcccg ggatcgccac aaggagcctg
    18061 cctccgtgac cgatgcacct gtgcggcccg atggccgcgc cttccccgac atgatggccg
    18121 cgcacccgtg ccccgtcttc agcgacgagg tgatggcgca ggcgcgttca atgagcggtg
//
LOCUS       DAORUQ010000047        18149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2729_length_18149_cov_6.788438, whole genome shotgun sequence.
ACCESSION   DAORUQ010000047 DAORUQ010000000
VERSION     DAORUQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_18149_cov_6.788438"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1515)
                     /gene="dnaB"
                     /locus_tag="PLW13_06070"
     CDS             complement(121..1515)
                     /gene="dnaB"
                     /locus_tag="PLW13_06070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQY69974.1"
                     /translation="MADSPFQQARNPGPVLDVSSLKIPPHSIEAEQSLLGGLMLVNET
                     WDRVMELVTASDFYRHEHRLIFEAMTALAAGSHPFDVITLSEHLNGIDELESAGGFAY
                     LGELAANTPSAANVQSYALIVRERSTMRQLIAAANEIATRNFNPEGRSGAELLEEAEK
                     RIAEISENRVSRGGPQAVNDLLRGAMGRIDELYASKSRITGLSTGYIDLDDRTSGLQR
                     SDLVIIAGRPSMGKTAFAMNLVEHAVLKQDKPVLVFSMEMPAESLIVRMLSSIGRIDQ
                     QRLRNGQLLQEDWPRLTAAVTALKDRPLFIDDTPALSPAEVRARARRLSREHGDIAMI
                     MIDYLQLMQVPGSSEGRTNEVSEISRSLKAVAKEFKCPMVALSQLNRSLEQRPNKRPV
                     MSDLRESGAIEQDADVIAFIYRDEVYHEDTPEKGVAEIIIGKQRNGPIGTVKLAFQRE
                     FTRFENLAHGAYEY"
     gene            complement(1608..2054)
                     /gene="rplI"
                     /locus_tag="PLW13_06075"
     CDS             complement(1608..2054)
                     /gene="rplI"
                     /locus_tag="PLW13_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQY69975.1"
                     /translation="MNVILLEKVGGLGNLGDQVKVAGGYGRNFLIPYGKAVSATADNI
                     ARFEARRAELEKAAAERKAAAAARAEKLAGLVVTIKANCGDEGKLFGSIGTRDIADAI
                     TATGTEVGKSEVRLPTGVIRNTGEFDIDLQLHAEVTQTIKLSVTPL"
     gene            complement(2092..2955)
                     /locus_tag="PLW13_06080"
     CDS             complement(2092..2955)
                     /locus_tag="PLW13_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69976.1"
                     /translation="MRALAEYVMRGRREAILVSTMAVVLPMFFWLGAAVVGLVTLRKG
                     VREGAFVMLWALLPAAVVAYFGEIMPVAALLGITVAAGVLRLTVSWSWALCTSALLGL
                     VLGGGLLTIGRGYLAQVEELFAELMAGIGEQAAQKGGEATVALTAPGASDIAGMFGLI
                     LGVTLVICLIIARWWQAALYNPGGFRVEFHALRLDRVQVLALLLTAFGLGALGGGLRL
                     WAWVPLLPLLFAGIALVHALAAARGRGGWLGLFYAALLVLAPLKQFIVVLAAMDSWLD
                     IRGRWLKKPGG"
     gene            complement(2993..3220)
                     /gene="rpsR"
                     /locus_tag="PLW13_06085"
     CDS             complement(2993..3220)
                     /gene="rpsR"
                     /locus_tag="PLW13_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQY69977.1"
                     /translation="MARYFRRRKFCRFTAEGVKEIDYKDLDVLREYINETGTIVPSRI
                     TGTKANYQRQLSVAIKRARFLALLPYTDQHR"
     gene            complement(3272..3694)
                     /gene="rpsF"
                     /locus_tag="PLW13_06090"
     CDS             complement(3272..3694)
                     /gene="rpsF"
                     /locus_tag="PLW13_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQY69978.1"
                     /translation="MRHYEIVFLVHPDQSEQVPGMIERYTQAVTKDGGKVHRLEDWGR
                     RQLMHLINKVHKAHYVLMNVEASDDAMKELETTFRFNDAILRHLIIRRDEAVTDESPI
                     MRAEREGRDRRSRGDDGERRRPRQEREDEPAEEEAESA"
     gene            complement(3906..4649)
                     /gene="rlmB"
                     /locus_tag="PLW13_06095"
     CDS             complement(3906..4649)
                     /gene="rlmB"
                     /locus_tag="PLW13_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQY69979.1"
                     /translation="MKERERIHGMHAVAALLKRSPERVESLAVLRGRRDRRLEELLAA
                     ARTQGIAVRELPREELDRLAPGVNQGVVAEVRAAAALDESYLFDELLPGLAGPALLLV
                     LDGVTDPHNLGACLRSADAFGAHAVIVPRDNSAPLNATARKVASGAADVVPLVSVTNL
                     ARTLRALQEAGVWIVGAAGEAGQGIAAVDLRGAVALVMGAEGSGMRRLTREHCDHLAS
                     IPMCGEVSSLNVSVATGVFLYEARRQRTP"
     gene            complement(4660..6999)
                     /gene="rnr"
                     /locus_tag="PLW13_06100"
     CDS             complement(4660..6999)
                     /gene="rnr"
                     /locus_tag="PLW13_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQY69980.1"
                     /translation="MAPRKKPGSERDPRQADEAARYDNPIASREFILATLDRAGVPLT
                     LDELAEQLDLEAEDRREALRRRLRAMERDGQLMCNRRGAYGAVSRMDLVRGKVQASRE
                     GYGFLVPAEGGEDLYLHGRQMRQVFHGDEVLARASGTDQRGRREGTIVEVLKRNTELM
                     VGRIRLAQGVNYVVAENPRIQHEVLVRDADCGDAREGQYVTVQILTQPATDAPPTGRV
                     VEVLGDHMAPGMEIDVSLRMYDIPCTWPPEVADEAAGFGPEPKRGDKARRFDLRALPF
                     VTIDGEDAKDFDDAVYCEKRRGGGWRLWVAIADVSHYVKTGTALDAEARRRATSVYFP
                     GRVVPMLPEELSNGLCSLKPDVDRLALVCEMEIGRDGALGKHWFFEAVIRSAARLTYT
                     RVAATLAGEDAGIAAELVPHLQTLHALYRALRAARDERGAIDFETTETRIVFGPDRKI
                     EEVVPVERNDAHKLIEECMLSANVATARFLQKHKMPGLYRVHEGPGGEKLKSLREFLG
                     ELGLSLRGGDKPQPGDYQQLIAAVQERPDRHVIETVMLRSLSQARYQPDNLGHFGLNY
                     PAYAHFTSPIRRYPDLLVHRAIRAVIRAPEESTQVRRVEGTKPLARQKSYPYELKQVV
                     ELGEHCSMAERRADEAVRDVMSWLKCEYLEDRVGDVFDGVVSGVTGFGLFVEIQEVYA
                     DGLVHVSSLQNDYYHFDASGHRLIGERTRRVYRLGDRIRVRIARVDLDERKVDLLIDE
                     DGAPGGTAGGARKERKAEGKGRKPRDQERGGSGSRRRRR"
     gene            7130..7216
                     /locus_tag="PLW13_06105"
     tRNA            7130..7216
                     /locus_tag="PLW13_06105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7164..7166,aa:Leu,seq:cag)
     gene            complement(7304..8791)
                     /locus_tag="PLW13_06110"
     CDS             complement(7304..8791)
                     /locus_tag="PLW13_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQY69981.1"
                     /translation="MAAGGTPRHEFIPPPPGEILGHPRPLWMLFGAEFWERFCYYGMR
                     ALLAVYVAATFFGHLPEGEANAQASLTYGGYTALVYATGILGGYIADTYLGYQRSIIV
                     GGALIAAGMCMLLIPDLQWFLVGLAVIVAGNGLFKPNISTMVGALYAPQDARRDSGFT
                     IFYMGINAGAFVAPIVCASVVGARFGYQYGFLAAGLGMVLGIVLFHLLAGMLGHIGKA
                     PSGREGARPVLVVLGGALAMVPLLWFLLSKSAVLGYVLVGLMLFLVVYFTASGIRCGD
                     RVQLQRYGAMLLLFLANCLFWALFEQAGSSLNFFARDFVDAPFHFTLFQSANPLFILL
                     LAPLFAMLWPVLDCRGRNPSIPRKFAIALIGVGLGFLLLALAIEATPPGAKVIWLFLA
                     LTYLIHTMAELCLSPIGLSMVTKLAARHEVGMAMGGWFLSTAMANYLAGLIAAVASGG
                     GGHGAAGSLAQYAGTFTQLWWLALLIGGGYFLCAPLINRLMHGVK"
     gene            8969..9877
                     /locus_tag="PLW13_06115"
     CDS             8969..9877
                     /locus_tag="PLW13_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQY69982.1"
                     /translation="MTKSKRELRENIFPSAREDAATSRRKAGRYSGNAYRLAFTDTEF
                     LLREELRPVRFQLELLKPDLIQQEHGIDSTVVIFGSARHASAERAEAELAAARDAVAA
                     RPRSPLAKAALARAERRLHNSRYYEEARKLSALITRNSEHIDGCRLVVVTGGGPGIME
                     AANRGAHEAGGKSIGLNIVLPMEQEPNPYITPELCFQFHYFALRKMHFLMRARALVAF
                     PGGFGTLDELFEALTLIQTGKSTRVPVLLFGEEFWRRLIDFDLLVEEGNIAAEDLGLF
                     RYVESAEQAWREIADFYSLGCPATLP"
     gene            complement(10067..10363)
                     /locus_tag="PLW13_06120"
     CDS             complement(10067..10363)
                     /locus_tag="PLW13_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQY69983.1"
                     /translation="MNIYVGNLPYTVRDNDLLEMFQTYGNVSSAKVVMDRETDRSKGF
                     GFVEMSSDDEGTNAINKLNGHEINGRALRVNEARPREDRRPGGGGGGDRRPKRW"
     gene            complement(10552..11850)
                     /locus_tag="PLW13_06125"
     CDS             complement(10552..11850)
                     /locus_tag="PLW13_06125"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020312432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQY69984.1"
                     /translation="MAKNVVVLGTQWGDEGKGKIVDLLTENVTAVVRFQGGHNAGHTL
                     VIGGQKTVLHLIPSGVLREGVLCLIGNGVVLSPEALLAEMAELESRGVPVRERLRLSP
                     ACPLILPHHAALDIARENARGDAKIGTTGRGIGPAYEDKAARRGLRLGDLRNAARFEA
                     KLREVMDYHNFVLREYYKVPQIDVQQTLEQSLRHGESLLPLMADVPALLHEYRMAGKR
                     ILFEGAQGALLDIDHGTYPFVTSSNTTAGGTATGSGFGPLYLDYVLGITKAYTTRVGS
                     GPFPTELFDEVGAHLARRGHEFGATTGRPRRCGWFDAVSLREVVRINSVSGLCITKLD
                     VLDGLETINMCVGYEDSSGHIVGTPRDSADFESLVPIYETVAGWEGSTIGITAWDQLP
                     LNARRYIERIAEVVGVGVDIVSTGPDRTETIILRDPFGRA"
     gene            complement(11864..13060)
                     /locus_tag="PLW13_06130"
     CDS             complement(11864..13060)
                     /locus_tag="PLW13_06130"
                     /inference="COORDINATES: protein motif:HMM:NF009086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQY69985.1"
                     /translation="MTGTDTSAAGEERWLLPEGIEELLPEQAARVECLRRDLLDLYGR
                     WGYELVIPPLMEFTDSLLIGLGKDLDLQTFTLTDQLSGRLLGIRPDITAQAARIDAHS
                     LRREGPVRLCYAGSVLHTRRRSPFASRSPIQLGAELYGDASAAADLEIISLMLETMCR
                     LGVRDVTLDLGHVGIFRALMQDLALDAAVQDAVFEALQHKSVPQIAAIVAEAGLDAAR
                     AGLLCRLAGLNGGREVIDRAAQLFAGAAPSACAPLAALADVADKIAARMPGVTLHFDL
                     CELQGYHYHTGLVFAAYVPGQGEAIANGGRYDDIGRVFGRARPATGFSFDLKTLLGLL
                     PATAAAPRRGVFVDHALADAAWTEIQRLREGGERVLLGLPGQQGSACCDRELRRAADA
                     WHIHTV"
     gene            complement(13084..13269)
                     /locus_tag="PLW13_06135"
     CDS             complement(13084..13269)
                     /locus_tag="PLW13_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HQY69986.1"
                     /translation="MWHDLAVAFCLMLVIEGIMPFLSPAGWRAMVLSVTGMSDRALRV
                     AGLCAMVAGTTLLYAIN"
     gene            complement(13363..14238)
                     /gene="hflC"
                     /locus_tag="PLW13_06140"
     CDS             complement(13363..14238)
                     /gene="hflC"
                     /locus_tag="PLW13_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQY69987.1"
                     /translation="MNARIVTLFLLLVVSLLALTGSLYVVRETERAVVLRFGALIDVD
                     SKPGLHVKIPFVDEVRKFDARVLTLDAPAESFYTLEKKRLIVNSFSKWRIDDVETYYR
                     ATGGDENAAMMRLAARVNDGLRNQFGKRKLHEVVSGEREKLMTDLIEELNAAVKQSLG
                     IEVIDVRVKKIDLPEEVSEAVFNRMSAEREKLAREYRSQGKEQAEKIRADADRQVTIM
                     EAEAYRDAELARGEGDAEASAIYAAAFDKDPEFYSFTRSLKAYESAFAGPEDVLVLDP
                     KSDFFRYLNESGGRR"
     gene            complement(14238..15377)
                     /gene="hflK"
                     /locus_tag="PLW13_06145"
     CDS             complement(14238..15377)
                     /gene="hflK"
                     /locus_tag="PLW13_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQY69988.1"
                     /translation="MAWNEPGGNGKQRDPWGGDQGPPDLDEAFRKFREKLGGALGGGG
                     TGDARFNSGVIGLVLLLIFGVWFYLGIYVIDEQERAVVLRFGRYHQTLDPGLNWKPRL
                     MDQVVPVNVTKERQYASEGRMLTQDENIVELQIVVQYNISDVKSFVLNVRDPEFSLRQ
                     ATESALRHVVGSTRLDQVLSEGRGQISDEVKVKLQSYLDNYGTGIQVVKITIQEARPP
                     AQVKAAFDDVIKAKEDEERLKNEASAYANGIVPEARGRAQRMLEEAAAYRGKVVAEAQ
                     GEAQRFEKLLAEYRKAPEVTRERLYLDAVQRVMSATSKVLVDVPGGNNVLYLPLDKMA
                     RPGAAAAAPRQGAAPPDTVGEVADEVVDRLRREAATGTGRARERR"
     gene            complement(15432..16727)
                     /gene="hflX"
                     /locus_tag="PLW13_06150"
     CDS             complement(15432..16727)
                     /gene="hflX"
                     /locus_tag="PLW13_06150"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQY69989.1"
                     /translation="MFFERPQSGERAVLVHLELGGGAAQEDPRELEELVLSAGGDPVE
                     FVFGQRDRPDPRHFVGTGKLAEIEAAVRAHDAALVIFNHALSPSQERNLERALGCRVI
                     DRTGLILDIFAQRARSHVGKLQVELAQLRHMSTRLVRGWTHLERQKGGIGLRGPGETQ
                     LETDRRLLRDRIRMLLRRLEKVHSQREQGRRARQRAEVPTVSLVGYTNAGKSTLFNTL
                     TGAGVYAADQLFATLDPTMRRCTLPDGRPMVLADTVGFVRHLPHQLVEAFRATLDEAR
                     LADLLLHVIDASAADREDTSFEVEKVLGEIGADKVVRLEVCNKIDLLQAAPRIDRGED
                     GKPVRVWVSAQSGAGLDLLREAIAELLEQDLVEECIPIGPAQGRLRARLYEKGAVLAE
                     HIDEQGQISLDIRLPRALLAQLLAAEKQAPAERPAAEHI"
     gene            complement(16729..16974)
                     /gene="hfq"
                     /locus_tag="PLW13_06155"
     CDS             complement(16729..16974)
                     /gene="hfq"
                     /locus_tag="PLW13_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQY69990.1"
                     /translation="MSKGQTLQDPFLNALRKERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAVSTVVPSRPVRLASADEGDEAAD"
     gene            complement(17121..18038)
                     /gene="miaA"
                     /locus_tag="PLW13_06160"
     CDS             complement(17121..18038)
                     /gene="miaA"
                     /locus_tag="PLW13_06160"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQY69991.1"
                     /translation="MGPTASGKTALALALADHLDCELISVDSAQVYRGMDIGTAKPEP
                     AVLARYPHRLIDIRDPAEPYSAAGFRRDALAAIAEILAAGRTPLLVGGTMLYFRALIH
                     GLADMPGADPAIRAGIAALAAREGWEAVHRRLAEVDPASAARIEPGNRQRVQRALEVF
                     LASGRPMSSLHAAQLPAPDLPCRLLQLALVPADRAVLHRRIAERFRAMLAAGFLDEVR
                     ALKARGDLHPDLPSIRSVGYRQAWDYLEGHVSREVMIERAVAATRQLAKRQLTWLRSW
                     PDLRILPADDPQSDKLLEMALKLVAVVAT"
BASE COUNT         2843 a         6312 c         6109 g         2885 t
ORIGIN      
        1 gcccgcggct tatgcccgct caggcggtgt gagtcgggtt tttacaacac cctctgaagc
       61 cggacccacg gtggagcgtc cggctgaagc cggacccacg aagagccgga cccacggggg
      121 tcagtattcg taggcgccgt gcgccaggtt ctcgaagcgc gtgaattcgc gctggaacgc
      181 gagcttgacg gtgccgatgg ggccgttacg ctgcttgccg atgatgatct cggccacgcc
      241 cttctccggc gtgtcctcgt ggtagacctc gtcgcggtag atgaacgcga tcacgtcggc
      301 gtcctgctcg atcgcgcccg attcgcgcag gtccgacatc acgggacgct tgttcggtcg
      361 ctgctccaat gagcggttga gctgcgacag cgcgaccatc gggcacttga attccttggc
      421 caccgccttg aggctgcgcg agatctcgga cacctcgttg gtgcgtccct cggaggaacc
      481 cggcacctgc atcagctgca ggtagtcgat catgatcatc gcgatgtcgc cgtgctcgcg
      541 cgagagccgc cgcgcgcgcg cgcgcacctc ggccggcgac agcgcaggcg tatcgtcgat
      601 gaacagcggc cggtccttca gcgcggtcac ggccgcggtg aggcgcggcc agtcctcctg
      661 cagcagctgg ccgttacgca ggcgctgctg gtcgatgcgc ccgatcgagg acagcatccg
      721 cacgatcagc gactcggccg gcatttccat cgagaacacc agcaccggct tgtcctgctt
      781 cagcaccgcg tgttcgacca ggttcatcgc gaaggccgtc ttgcccatcg aggggcggcc
      841 cgcgatgatc actaggtccg agcgctgcag cccggaagtg cggtcgtcga ggtcgatgta
      901 gccggtgctg agccccgtga tgcggctctt gctggcgtag agctcgtcga tgcggcccat
      961 cgcgccgcgc agcaggtcgt tgacagcctg cgggccgccc cgcgacacgc ggttctcgct
     1021 gatctccgcg atgcgcttct cggcttcctc gagcagttcc gccccgctgc gtccctcggg
     1081 gttgaagttg cgcgtggcga tctcgttggc ggcggcgatc agttggcgca tcgtggagcg
     1141 ctcgcgcacg atcagcgcgt aggactgcac gttagccgcg ctgggcgtgt tggcggcgag
     1201 ttcgccgaga taggcgaagc cgccggcgct ctcgagctcg tcgatgccgt tgaggtgctc
     1261 cgagagcgtg atgacgtcga aggggtggct gcccgcggcc agcgcggtca tcgcctcgaa
     1321 gatcaggcgg tgttcgtgac ggtagaaatc ggacgcggtg accagttcca tgacccggtc
     1381 ccaggtctcg ttcacgagca tcaggccgcc gagcagcgac tgctcggctt caatggagtg
     1441 cggcgggatc ttcaggctgc tgacgtccag cacgggcccg gggttccttg cctgctggaa
     1501 cggtgaatca gccatggtgc gcgcccgcag atctgagggc gcgatgttcc caccgcgcaa
     1561 aaatggctgt caaccgcagc cggccgacag ccatggcggc gccgtcctca gagcggagtg
     1621 acggacagct tgatggtctg cgtgacttcg gcgtgcagct gcaggtcgat gtcgaactcg
     1681 ccggtgttgc ggatcacgcc ggtcggcagg cgcacttcgc tcttgcccac ttcggtgccc
     1741 gtcgcggtga tcgcatcggc gatgtcgcgg gtgccgatcg agccgaacag cttgccttcg
     1801 tcgccgcagt tggccttgat cgtgaccacg aggcccgcga gcttctcggc gcgcgccgcg
     1861 gcggctgcct tgcgctcggc ggcggctttc tcgagctcgg cgcggcgcgc ctcgaagcgc
     1921 gcgatgttgt cggcggtcgc cgaaacggcc ttgccgtagg ggatcaggaa gttgcggccg
     1981 taaccgcccg ctaccttgac ctggtcgccc aggttgccca gtccaccaac tttctcgagc
     2041 agaatgacgt tcattgctat cgtcctcacc agattcccga attcatcgac ttcagccgcc
     2101 gggcttcttc agccagcggc cgcgtatatc cagccagctg tccatcgcgg ccagtaccac
     2161 gatgaactgc ttgagcggcg ccagcaccag cagcgccgcg taaaacaatc cgagccaccc
     2221 gccgcgtccg cgcgcggcgg ccagggcatg caccagcgcg atgcccgcaa acagcagcgg
     2281 cagcagcggc acccaggccc acagccgcaa gcccccgccc agcgcgccga ggccgaaggc
     2341 cgtcagcagc agcgccagca cctgcacccg gtcgaggcgc agcgcgtgga actccacgcg
     2401 aaagccgccc gggttgtaca gcgccgcctg ccaccagcgc gcgatgatca ggcagatcac
     2461 cagcgtcacg ccgaggatca gtccgaacat gccggcgatg tcgctcgccc cgggggccgt
     2521 gagcgccacc gttgcctcac cgcccttctg cgccgcctgc tcaccgattc cggccatcag
     2581 ctcggcgaac agctcctcga cctgcgcgag atagccgcgc ccgatcgtga gcaggccgcc
     2641 acccagcacc aggccgagca gcgccgaggt gcacagcgcc cacgaccatg acaccgtgag
     2701 gcgcaagacc cctgccgcca ccgtgatgcc gagcagcgcg gccacgggca tgatctcgcc
     2761 gaagtacgcc accaccgctg ccggcaacag ggcccacagc atcacgaaag cgccttcgcg
     2821 cacgcccttg cgcagcgtga ccagaccgac cactgctgcc ccgagccaga agaacatggg
     2881 caatacgacg gccatcgtgg aaaccagaat ggcttcccgg cgcccgcgca tcacgtactc
     2941 ggcaagggca cgcatacctt gaattcccga cccgcccggg ggcgggccgc tgtcagcggt
     3001 gctggtccgt gtagggcagc agcgccagga aacgagcgcg cttgatcgcg acggacagct
     3061 ggcgctggta gttcgccttg gtccccgtga tgcggctcgg cacgatggtg ccggtctcgt
     3121 tgatgtattc gcgcaggacg tcgagatcct tgtaatcgat ctctttcacg ccttcggcgg
     3181 tgaaacggca aaacttgcga cgacggaaat aacgggccat tggcctgact cctcgattct
     3241 actgatacct gagtgggaaa ccggtcgcga cttacgcgga ctcggcttct tcttccgcgg
     3301 gctcgtcctc gcgctcctgg cgcgggcggc gacgctcgcc gtcatcgcca cggctgcggc
     3361 gatcgcgacc ttcgcgctcg gcgcgcatga tcggcgattc gtcggtgacc gcttcgtcgc
     3421 ggcggatgat caggtggcgc aggatcgcgt cgttgaagcg gaaagtcgtt tccagctcct
     3481 tcatcgcgtc atcggaagct tcgacgttca tcagcacgta gtgtgccttg tgaaccttgt
     3541 tgatgaggtg catcagctgg cgacgccccc agtcttccag gcggtgtacc ttgccgccgt
     3601 ccttcgtcac ggcctgggtg tatctttcga tcatgccagg cacctgttcg ctctggtccg
     3661 gatggaccag gaacacgatt tcgtagtgtc gcattgtcac tccctgcggt ttgaacagcc
     3721 tcccgcaacg gcggtgaggc agggatttcc cccgcggctg gcgcaaggga gggcggattc
     3781 tagagaagtg gacccggcgg cgcaatccgg gcggggcaaa ttgtgcaaat ttcgcccaac
     3841 gcccgtaggt cgccattcat ggcgacattg ccttgcgaat cacatttgtc gccattcatg
     3901 gcgacctacg gggtgcgctg gcggcgggcc tcgtagagga acacgccggt ggcgaccgat
     3961 acgttgaggc tgctgacctc gccgcacatc gggatcgagg cgaggtgatc gcaatgctcg
     4021 cgcgtgaggc gacgcatgcc gctgccctcg gcccccatca ccagcgccac cgcgccgcgc
     4081 aggtccaccg cggcgatgcc ctgcccggcc tcgccggcgg cgcccacgat ccacaccccg
     4141 gcctcctgca gcgcgcgcag cgtgcgcgcg aggttggtga cgctgaccaa cggcaccacg
     4201 tcggccgcgc ccgaggccac cttgcgcgcc gtggcgttca gcggcgcgga gttgtcgcgc
     4261 ggcacgatca ccgcgtgcgc cccgaaggca tccgccgagc gcaggcacgc accgaggttg
     4321 tgcggatcgg tcacgccatc cagcaccagc agcagcgccg ggcccgcgag ccccggcagc
     4381 agctcgtcga acaggtagct ctcgtccagg gccgcggcgg cgcgcacctc ggcgaccacc
     4441 ccctggttca cgcccggcgc gagccggtcg agctcctcgc gcgggagctc gcgcacggcg
     4501 atgccctgcg tgcgcgccgc cgccagcagt tcctcgaggc gccggtcgcg ccgcccgcgc
     4561 agcaccgcca gcgattccac gcgctcgggg ctgcgcttga gcagcgcggc caccgcgtgc
     4621 atgccgtgga tgcgttcgcg ctccttcacc ggggcctcct cagcgccggc gccggcgcga
     4681 cccggagccg ccgcgctcct ggtcccgagg cttgcgcccc ttgccctcgg ccttgcgctc
     4741 cttgcgcgct ccacccgctg tgccacccgg cgcgccgtcc tcgtcgatca gcaggtcgac
     4801 cttgcgctcg tcgaggtcca cgcgcgcgat gcgcacgcgg atacggtccc cgaggcggta
     4861 gacgcggcgc gtgcgctcgc cgatcaggcg gtggccggag gcatcgaagt ggtaatagtc
     4921 gttctgcagg ctggaaacgt gcaccagccc gtcggcatag acctcctgga tctcgacgaa
     4981 cagcccgaag ccggtgacgc cgctcaccac gccgtcgaac acgtcgccga cgcggtcctc
     5041 gaggtattcg cacttcaacc agctcatcac gtcgcgcacg gcctcgtcgg cgcgccgctc
     5101 ggccatcgag cagtgctcgc cgagctcgac cacctgcttg agctcgtagg ggtaggactt
     5161 ctggcgcgcg aggggtttcg tgccctcgac gcggcgcacc tgggtgctct cttccggcgc
     5221 gcggatcacc gcgcggatcg cgcggtgcac cagcaggtcg gggtagcggc ggatcggcga
     5281 ggtgaagtgc gcataggccg ggtagttcag cccgaaatgg ccgaggttgt cgggctggta
     5341 gcgcgcctgg gagagcgagc gcagcatcac cgtctcgatc acgtggcgat cggggcgctc
     5401 ctgcaccgcg gcgatcagct gctggtagtc gcccggctgc ggcttgtcgc cgccgcgcag
     5461 cgacagcccg agctcgccga ggaactcgcg cagcgacttc agcttctcgc caccgggccc
     5521 ctcgtgcacg cggtacagcc cgggcatctt gtgcttctgc aggaagcgcg cggtggcgac
     5581 gttggccgag agcatgcact cctcgatcag cttgtgggcg tcgttgcgct ccaccggcac
     5641 cacttcctcg atcttgcggt cggggccgaa cacgatgcgt gtctcggtgg tctcgaagtc
     5701 gatcgcgccg cgctcgtcgc gcgccgcgcg cagggcgcga tagagcgcat gcagggtctg
     5761 gaggtgcggc accagttccg cggcgatgcc ggcatcctcg ccggccagcg tcgcggccac
     5821 gcgggtgtag gtgagccgcg cggccgagcg gatcaccgcc tcgaagaacc agtgcttgcc
     5881 gagcgcgccg tcacgcccga tttccatctc gcagaccagc gcgaggcggt cgacatcggg
     5941 cttgagcgag cacaacccgt tcgagagctc ctccggcagc atcggcacca cgcgccccgg
     6001 gaagtagacc gaggtggcgc gccggcgcgc ctcggcatcg agcgcggtgc cggtcttcac
     6061 gtagtgcgag acgtccgcga tcgccaccca cagccgccag ccgccgccgc ggcgcttctc
     6121 gcaatagacg gcgtcgtcga agtccttggc gtcctcgccg tcgatggtca cgaagggcag
     6181 ggcgcgcagg tcgaagcgcc tggccttgtc gccacgcttg ggttccggac cgaagcccgc
     6241 cgcctcgtcc gccacctccg gcggccaggt gcaggggatg tcgtacatgc gcagcgagac
     6301 gtcgatctcc atgcccggcg ccatgtgatc gcccagcacc tcgacgacgc gcccggtcgg
     6361 cggtgcatcg gtcgccggct gcgtcaggat ctgcaccgtg acgtactgcc cctcgcgggc
     6421 atcgccgcag tcggcgtcgc gcaccagcac ctcgtgctgg atgcgcgggt tctcggccac
     6481 cacgtagttc acgccctgcg cgaggcggat gcgcccgacc atgagctcgg tgttgcgctt
     6541 gagcacctcg acgatggtgc cctcgcggcg cccgcgctgg tcggtgccgc tggcgcgcgc
     6601 cagcacctcg tcgccgtgga agacctggcg catctgccgc ccgtgcaggt acaggtcctc
     6661 gccgccctcg gccggcacca ggaagccgta gccctcccgg ctcgcctgca ccttgccgcg
     6721 caccaggtcc atgcgcgaga ccgcgccgta ggcgccgcgg cggttgcaca tcaattgtcc
     6781 gtcgcgctcc atcgcgcgca ggcgccggcg cagggcctcg cgccggtcct cggcctcgag
     6841 gtccagctgt tcggccagct cgtccagggt gagcggtacg ccggccctgt cgagcgtggc
     6901 aaggatgaac tcgcggctcg cgatcgggtt gtcgtagcgt gcggcttcgt cggcctggcg
     6961 cgggtcgcgc tcgctgccgg gtttcttgcg tggggccaag ggtgttctcc aggatgcggc
     7021 atggccgcga tataacgtgc gcgcattata cgcacaggga ttgacacagc ggatgccgct
     7081 cagtagagtg cgcgccctgt tcgcgggaga atttcccgcc agcccctagg cccaggtggc
     7141 ggaactggta gacgcgctag cttcaggtgc tagttatcga aaggtagtgg aggttcaagt
     7201 cctctcctgg gcaccatctc tctcctcgac gttgtgtact cccggctccg gacagcgtcg
     7261 ctgcccggaa ccgggatgtt tttttccgtc ggcgtcgccc ccgctacttc accccgtgca
     7321 tcaggcgatt gatcagcggc gcgcacagga agtagccgcc accgatcagc agtgcgagcc
     7381 accacaactg cgtgaaggtc ccggcgtatt gcgcgagcga tccggccgcg ccgtgcccgc
     7441 cgccaccgga ggcaaccgcg gcgatcaggc cggcgagata attcgccatc gcggtcgaca
     7501 ggaaccaccc gcccatcgcc atgccgacct cgtgccgtgc cgccagcttg gtgaccatcg
     7561 acagcccgat cggcgacagg cagagctcgg ccatggtgtg gatgaggtag gtcagggcca
     7621 gaaacagcca gatcaccttt gcccctggcg gggtggcctc gatggccagc gccagcagca
     7681 ggaagcccaa cccgaccccg atcagcgcga tcgcgaactt gcgcggaatc gagggattgc
     7741 gcccccgaca gtccagcacc ggccacagca tcgcgaacaa cggcgccagc agcaggatga
     7801 acagcgggtt ggccgactgg aacagcgtga agtggaacgg cgcatcgacg aaatcgcgcg
     7861 cgaagaaatt gagcgacgag ccggcctgct cgaacagcgc ccagaacagg caattcgcca
     7921 ggaacagcag cagcatcgcg ccgtagcgct gcagctgcac gcggtcaccg catcggatcc
     7981 cgctggcagt gaaatacacc accaggaaca gcatcaggcc gaccagcacg tagcccagca
     8041 ccgcgctctt gctcagcagg aaccacagca gcggcaccat cgcgagcgcg ccgcccaaca
     8101 cgaccagcac gggcctggcg ccctcgcgcc cggacggcgc cttgccgata tggccgagca
     8161 tgcccgcgag caagtggaac agcacgatgc cgagcaccat gccgaggcca gccgccagga
     8221 agccgtactg gtagccgaac ctggcgccga caacggacgc gcagacgatc ggcgcgacga
     8281 aggcgccggc attgatgccc atgtagaaga tcgtgaaccc ggagtcacgc ctggcgtcct
     8341 gcggcgcgta gagcgcaccg accatggtgg agatgttggg cttgaacagg ccgttgccgg
     8401 ccactatcac ggccagcccc accaggaacc actgcaggtc cgggatcagc agcatgcaca
     8461 tgcccgccgc gatcagcgcg ccgccgacga tgatcgatcg ctggtagccg aggtaggtat
     8521 cggcgatgta accgccgagg atcccggtgg cgtagaccag cgccgtgtaa ccgccatagg
     8581 tgaggctggc ctgcgcattg gcctcgccct cgggcaggtg gccgaaaaag gtcgcggcca
     8641 cgtataccgc cagcagggcg cgcatgccgt agtagcagaa gcgctcccag aactcggcgc
     8701 cgaacagcat ccacagcggc ctggggtgcc cgagtatctc gccggggggc ggggggatga
     8761 actcgtgcct cggtgtgccg cccgccgcca tgcgcgtctc ctgcgttgct gttcgtggtg
     8821 atgcacccgc aacgacggca tgtgcgtccg ggtgtgttta gcctgcgccc ggaggcaagt
     8881 caatcgatgc cggcatgcgc gcgcgccccg catcgggcgt ggcgctgggc cataatcggc
     8941 gactccggcg atcattcagg agctgtgcat gacgaagagc aagagggagc tgcgcgaaaa
     9001 catattcccc agcgcgcgcg aggacgccgc gacgtcgcgc cgcaaggcgg ggcgatacag
     9061 cggcaatgcc taccgcctcg cgttcaccga cacggagttc ctgctgcgcg aggaacttcg
     9121 cccggtgcgc ttccagctcg agttgctcaa gcccgacctg atccagcagg agcacggcat
     9181 cgattccacg gtggtcatct tcggcagtgc gcgccacgcg tcggccgagc gggccgaggc
     9241 cgagctcgcc gcggcgcgcg acgcggtcgc ggccaggccg cgcagccccc tggcgaaagc
     9301 cgcgctggcg cgcgccgagc gccggctgca caacagccgc tactacgagg aggcgcgcaa
     9361 gctcagcgcg ctcatcacgc gcaacagcga gcacatcgac ggctgccggc tggtggtggt
     9421 gacgggcggc ggaccgggaa tcatggaggc ggcgaaccgc ggcgcgcacg aggccggcgg
     9481 caagagcatc ggcctgaaca tcgtgttgcc gatggagcag gagcccaacc cctacataac
     9541 gcccgaactg tgcttccagt tccactactt cgcgctgcgc aagatgcact tcctgatgcg
     9601 cgcgcgggcc ctggtcgcat ttcccggcgg cttcggcacg ttggacgagt tgttcgaggc
     9661 attgacgctg atccagaccg gcaagtcgac gcgcgtgccc gtgctgctgt tcggggagga
     9721 gttctggcgc cggctgatcg acttcgacct gctggtcgag gaaggcaata tcgcggccga
     9781 ggacctcggg ctgttccgct acgtggagag cgcggagcag gcctggcggg agatcgcgga
     9841 tttctacagc ctgggctgcc cggcaaccct gccgtgaagc gcgcagggag cgggtcggcc
     9901 agcgaattgc ggaatgacgc aacccttccg cccctgcgac aggacgagtc cgggcaaaca
     9961 agcagggccg gcggcaccat ccaccaacgg ctgccttcag gaaggtgcca cgggctggtg
    10021 gcccgcggcc gcggtcaggg gcggcgcgtc cgtgcgccgc cggatctcac cagcgcttcg
    10081 gacggcgatc gccgccaccg ccgccgccag gacggcggtc ttcgcggggg cgcgcttcgt
    10141 tcacgcgcag cgcacggccg ttgatctcgt ggccgttcag cttgttgatg gcattggtgc
    10201 cctcgtcgtc ggaggacatc tccacgaaac cgaagccctt cgaacgatcg gtttcacgat
    10261 ccatcacgac ctttgccgag gacacgttcc cgtaggtctg gaacatctcg agcaagtcgt
    10321 tgtcccgaac ggtgtagggc aggtttccaa cgtagatatt cattcgcttc atcatcctgg
    10381 cgccgtggca gactggaagt gctaataccc cgacgcccga aagaaagcgt tagcaccagt
    10441 gaaaaccgga ctctcgacga gtccggcccg gcgcattcta actgaaaatg caactacgtc
    10501 cagcacccgg ggcttgacag ccgggcaacg ggcgctccgg gaaaccgggc ctcaggcccg
    10561 gccgaacggg tcgcgcagga tgatggtctc ggtgcggtcg ggtccggtgg acacgatgtc
    10621 cacgccgacg ccgacgacct cggcgatgcg ctcgatgtag cggcgcgcgt tgagcggcag
    10681 ctgatcccac gcggtgatgc cgatcgtgga gccttcccag cccgcgacgg tttcgtagat
    10741 cgggaccagg ctctcgaaat ccgcgctgtc gcgcggcgtg cccacgatat ggccggagct
    10801 gtcctcgtag cccacgcaca tgttgatggt ctcgaggccg tcgagcacgt cgagcttggt
    10861 gatgcacagc ccgctgacgc tgttgatgcg caccacttcg cgcagcgaca ccgcgtcgaa
    10921 ccagccgcag cggcgcggcc gtccggttgt cgcgccgaat tcgtgcccgc gccgcgcgag
    10981 atgggcgccc acctcgtcga acagctcggt cgggaacggg ccggatccga cccgcgtggt
    11041 gtaggccttg gtgatgccga gcacgtagtc gaggtacagc gggccgaagc cgctgccggt
    11101 ggcggtgccg ccggcggtgg tgttcgacga cgtcacgaag ggataggtgc cgtggtcgat
    11161 gtcgagcagc gcgccctgcg cgccctcgaa caggatgcgc ttgccggcca tgcggtattc
    11221 gtgcagcagc gccggcacgt cggccatcaa cggcagcagc gactcgccgt ggcgcaggct
    11281 ctgttcgagc gtctgctgca catcgatctg cggcaccttg taatactcgc gcagcacgaa
    11341 gttgtggtaa tccatcacct cgcgcagctt ggcttcgaag cgcgcggcgt tgcgcaggtc
    11401 gccgaggcgc agcccccgcc gtgccgcctt gtcctcgtag gccggaccga tgccacgccc
    11461 cgtggtgccg atcttcgcat cgccgcgcgc gttctcgcgc gcgatgtcga gcgccgcgtg
    11521 atggggcagg atcagggggc aggccgggct gaggcgcagg cgctcgcgca ccggcacgcc
    11581 gcgcgattcg agctcggcca tctcggcgag cagcgcctcg ggcgacagca cgacgccgtt
    11641 gccgatcagg cacagcacgc cctcgcgcag cacgcccgaa gggatcaggt gcagcaccgt
    11701 cttctgcccg ccgatcacca gcgtgtggcc ggcattgtga ccgccctgga aacgcaccac
    11761 cgcggtgacg ttctcggtca gcaggtcgac tatcttgccc ttgccctcgt caccccattg
    11821 cgtgccgagg accactacgt tttttgccat gtcccgatgc ctgtcagact gtgtggatgt
    11881 gccatgcgtc ggccgcgcga cgcaactccc gatcgcaaca tgcgctgccc tgctggccgg
    11941 gcagccccag caatacccgc tcgccgccct cgcgcaggcg ctggatctcg gtccaggccg
    12001 catccgccag cgcgtgatcg acaaacaccc cgcggcgcgg cgcggcggct gtggccggca
    12061 gcaggccgag cagggtcttg aggtcgaaac tgaagccggt cgccggacgg gcgcgcccga
    12121 acacgcgccc gatgtcgtcg tagcgcccgc cattggcgat cgcctcgccc tggcccggca
    12181 cgtaggccgc aaacaccagg ccggtgtggt agtgatagcc ctgcagctcg cacagatcga
    12241 aatgcagggt caccccgggc atgcgcgcgg cgatcttgtc ggccacgtcg gcgagcgcgg
    12301 cgagcggcgc gcaggccgat ggcgccgccc cggcgaacaa ctgcgcggcg cgatcgatca
    12361 cctcgcgccc gccgttcagt cccgccagcc ggcacagcag gccggcgcgc gccgcgtcga
    12421 ggccggcttc ggcgacgatc gccgcgatct gcggaaccga cttgtgctgc aacgcctcga
    12481 acacggcatc ctgcaccgcg gcgtccagcg ccaggtcctg catcagggcg cggaagatgc
    12541 ccacgtgccc gaggtcgagc gtcacgtcgc gcacgccgag gcggcacatc gtttccagca
    12601 tcaggctgat gatctcgaga tccgcggccg cgctggcgtc gccgtagagc tcggcgccga
    12661 gctggatcgg agagcgcgag gcgaagggcg agcgccggcg cgtgtgcagc acgctgcccg
    12721 cgtagcacag gcgcaccgga ccctcgcggc gcaggctgtg ggcgtcgatg cgcgcggcct
    12781 gcgcggtgat gtcggggcgg atgccgagca ggcgccccga gagctggtcg gtcagggtga
    12841 aggtctgcag gtcgaggtcc ttgcccaacc cgatcagcag cgaatcggtg aactccatca
    12901 gcgggggaat caccagctcg tagccccagc ggccgtaaag gtccagcaga tcgcgccgca
    12961 aacactcgac gcgcgccgcc tgctcgggga gcagttcctc gatcccctcc gggagcagcc
    13021 agcgctcttc gccggcggcg gacgtatcgg ttccggtcat cgcgaatcct gtggagcgtc
    13081 ggttcagttg atggcgtaca gcagggtcgt gccggcgacc atggcgcaca gtcccgcgac
    13141 gcgcagcgcc cggtcgctca tcccggtgac ggaaagcacc atggcccgcc agcccgcagg
    13201 cgacaggaac ggcatgatcc cctcgatgac gagcatcagg cagaacgcta cggcaagatc
    13261 gtgccacatc tggcccggga cccggtgaac gcgttgcggg atgcgcccgc ctcggcgggc
    13321 gcggcgcatc ctagcacaaa gccggccgtg atcgaatccg cgctagcgcc ggccgccgga
    13381 ttcgttcagg tagcggaaga aatcgctctt cggatcgagc accagcacgt cctcgggtcc
    13441 ggcgaaggcg ctctcgtagg ccttgaggct gcgggtgaac gagtagaact ccgggtcctt
    13501 gtcgaaggcc gcggcataga tggccgaggc ctcggcatcg ccctcgccgc gcgcgagctc
    13561 ggcatcacga taggcctccg cctccatgat cgtgacctgg cgatcggcgt cggcgcggat
    13621 cttctcggcc tgctccttgc cctgggagcg gtattcgcgc gcgagcttct cgcgctcggc
    13681 cgacatgcgg ttgaacaccg cctcgctgac ctcctccggc aggtcgatct tcttcacccg
    13741 cacatcgatc acctcgatgc cgagcgactg cttaacggct gcgttcagtt cctcgatgag
    13801 gtcggtcatc agcttctcgc gctcgcccga gaccacctcg tgcagcttgc gcttgccgaa
    13861 ctggttgcgc aggccgtcgt tgacgcgcgc cgcgagccgc atcatcgcgg cgttctcgtc
    13921 gccgccggtg gcgcggtagt aggtctcgac gtcgtcgatg cgccacttcg agaacgaatt
    13981 gacgatcagg cgcttctttt ccagcgtgta gaagctctcg gccggcgcat ccagcgtcag
    14041 cacccgcgcg tcgaacttgc gcacctcgtc gacgaaggga atcttcacgt gcaggccggg
    14101 cttgctgtcg acgtcgatca gcgcgccgaa gcgcagcacc acggcgcgct cggtttcgcg
    14161 caccacgtac agcgatcccg tcagcgccag cagcgagacg accagcagca ggaacagggt
    14221 aactatcctc gcgttcatca gcgtcgctcc cttgcacgac cggtgcccgt tgcggcctca
    14281 cgccgcaacc tgtccacgac ctcgtccgcg acctcgccga ccgtgtcggg gggcgcggcg
    14341 ccctggcgcg gcgcggcggc ggctgcgccg ggccgcgcca tcttgtcgag cggcagatag
    14401 agcacgttgt tgcccccggg cacgtccacc agcaccttgg acgtcgcgct catcacgcgc
    14461 tgcacggcat ccaggtagag acgctcgcgc gtgacctcgg gcgccttgcg gtattcggcg
    14521 agcagcttct cgaagcgctg cgcctcgccc tgggcctcgg cgacgacctt gccgcggtac
    14581 gcagcggcct cctcgagcat gcgctgggcg cgaccgcgcg cctccggcac gatgccgttg
    14641 gcgtaggcgc tggcctcgtt cttcagccgc tcctcgtctt ccttggcctt gatcacgtcg
    14701 tcgaacgcgg ccttgacctg cgcgggcggc cgggcttcct ggatcgtgat cttcacgacc
    14761 tggatgccgg tgccgtagtt gtccaggtag ctctgcagct tcaccttgac ctcgtcgctg
    14821 atctggccgc ggccctcgga cagcacctgg tcgagccggg tgctgccgac cacgtggcgc
    14881 agcgcgctct cggtggcctg gcgcaggctg aactcgggat cgcgcacgtt cagcacgaag
    14941 gacttcacgt cgctgatgtt gtactgcacc acgatctgca actcgacgat gttctcgtcc
    15001 tgcgtgagca tccggccctc ggaggcgtac tggcgctcct tggtcacgtt gaccggaacc
    15061 acctggtcca tcagccgcgg cttccagttg aggccggggt ccagcgtctg gtgatagcgg
    15121 ccgaagcgca gcaccaccgc gcgctcctgt tcgtcgatca cgtagatgcc gaggtagaac
    15181 cacacgccga agatcagcag caggacgagg ccgatcacgc cgctgttgaa gcgtgcatcc
    15241 cccgtgccgc cgccccccag cgcacccccg agcttttcgc ggaacttgcg gaacgcctcg
    15301 tcgagatccg gcgggccctg gtcgccgccc cacggatctc gctgtttgcc gttgccacct
    15361 ggttcattcc aggccatgtc gttctcccac cggttattgg ttgcacgtgc gcaatagagc
    15421 gccggattct atcagatgtg ttccgcggcc ggccgctctg cgggcgcctg cttctccgcg
    15481 gcaagcagtt gtgcgagcag cgcgcgcggc aggcggatat cgagactgat ctgcccctgc
    15541 tcgtcgatgt gctcggcgag caccgcgccc ttctcgtaca gccgggcgcg cagacgcccc
    15601 tgcgccgggc cgatgggaat gcactcctcg accaggtcct gctcgagcag ctcggcgatc
    15661 gcctcgcgca acaggtccag ccccgcgccg ctttgcgccg acacccatac ccgcaccggc
    15721 ttgccgtcct cgccgcggtc gatacgcggc gcggcttgca gcagatcaat cttgttgcag
    15781 acctcgagcc ggaccacctt gtcggcgccg atctcgccga gcaccttttc gacctcgaag
    15841 gaggtgtcct cgcggtcggc ggcgctggcg tcgatcacgt gcagcaacag gtcggcgagg
    15901 cgcgcttcat cgagcgtcgc acggaaggcc tcgaccagct ggtgcggcag gtgacgcacg
    15961 aaacccaccg tgtccgcgag caccatcggg cgaccgtccg ggagcgtgca gcggcgcatc
    16021 gtgggatcga gggtggcgaa gagctggtcg gccgcgtaga cgccggcgcc cgtcagcgtg
    16081 ttgaacagcg tcgacttgcc ggcgttggtg tagccgacca gggacaccgt gggcacttcc
    16141 gcgcgctggc gcgcacggcg cccctgttcg cgctgggagt ggaccttttc caggcggcgc
    16201 agcagcatgc ggatgcgatc gcgcagcagg cggcggtcgg tttccagctg cgtctcgccg
    16261 ggtccgcgca ggccgatacc gcccttctgg cgttccaggt gggtccagcc gcgcaccagg
    16321 cgcgtggaca tgtggcgcag ctgcgccagc tcgacctgca gcttgcccac gtgcgagcgc
    16381 gcgcgctgcg cgaagatgtc gaggatcagc ccggtgcggt cgatcacgcg gcagccgagc
    16441 gcccgttcca ggttgcgttc ctgcgaggga ctcagggcat ggttgaaaat gaccagcgcc
    16501 gcgtcgtgcg cccggacggc cgcctcgatc tcggccagct tgccggtgcc aacgaaatgc
    16561 ctgggatccg ggcgatcgcg ctgcccgaac acgaactcca ccggatcgcc gcccgcggac
    16621 agcaccagct cctcgagttc acggggatct tcctgcgcgg cgccgccccc cagttcgagg
    16681 tgaacgagca ccgcgcgctc accgctctgc gggcgttcga agaacacctt agtcggcggc
    16741 ctcgtcgccc tcgtcggcag aggccaggcg caccggacgc gaaggcacca cggtcgatac
    16801 cgcgtgcttg tagaccatct ggctgacggt gttcttcagc aggatcacga actggtcgaa
    16861 ggattcgatc tggccctgca gcttgatgcc gttcaccagg tagatggaaa ccgggatgcg
    16921 ctccttgcgc agcgcgttca ggaaagggtc ttgtaaggtt tgcccctttg acattaaagc
    16981 attctcctag tgcagctccc cgcggagctg attgcattga agcccgatca ttcggacgcg
    17041 tttcaggcca tttgacccga atgcatggta cgagcgcaga atagaatgcg cattctaaag
    17101 cattccgcac tcgttcgacc ctaggtggcg acgactgcga cgagtttcaa ggccatctcg
    17161 agcaatttgt cggattgcgg gtcgtcggcc ggcaatattc gcaggtccgg ccacgagcgc
    17221 agccacgtga gctggcgctt ggcgagttgc cgcgtcgcgg cgaccgcgcg ctcgatcatc
    17281 acctcgcgcg agacatgccc ctccaggtaa tcccaggcct ggcggtaacc gacgctgcgg
    17341 atcgagggca ggtccgggtg caggtcgccg cgcgccttca gggcccgcac ctcgtccagg
    17401 aagccggcgg ccagcatcgc gcggaagcgc tcggcgatac gccggtgcag caccgcgcga
    17461 tccgcgggca cgagcgcgag ctgcagcagg cgacagggca ggtctggcgc cgggagctgc
    17521 gcggcatgca ggctgctcat cgggcggcca ctggccagga agacctcgag cgcgcgctgc
    17581 acgcgctggc ggttgccggg ctcgatgcgc gccgcactcg ccggatccac ctcggcgagg
    17641 cggcggtgca cggcttccca accctcgcgc gccgccagcg ccgcgatgcc ggcgcggatc
    17701 gccgggtcgg cacccggcat gtccgcgagc ccgtggatca gcgcgcggaa gtacagcatg
    17761 gtgccgccca ccagcagcgg cgtgcggccc gcggcgagaa tctccgcgat cgcggccagc
    17821 gcgtcgcggc ggaacccggc cgccgaatag ggctcggccg gatcgcggat gtcgatcagg
    17881 cggtgcgggt agcgcgcgag cacggccggc tcgggcttgg cggtgccgat gtccatgccg
    17941 cggtagacct gcgccgagtc gacgctgatc agctcgcaat ccaggtggtc ggcgagcgcc
    18001 agcgccagcg cggtcttgcc cgaggcggta ggccccatca ggcagatggc gagcgggcgc
    18061 cggtcggggc cgtccacggt cgtgctcatc ggccgcgcag gaacagccgg tcgagctccg
    18121 ccagcgactg cagcgcccag gtcgggcgg
//
LOCUS       DAORUQ010000048        18041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2756_length_18041_cov_6.030579, whole genome shotgun sequence.
ACCESSION   DAORUQ010000048 DAORUQ010000000
VERSION     DAORUQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18041
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_18041_cov_6.030579"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1010)
                     /locus_tag="PLW13_06165"
     CDS             complement(114..1010)
                     /locus_tag="PLW13_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY69992.1"
                     /translation="MPVTTAWIDAFLSRERLPSQFRTLIAEVHAPIAARVATEARRRG
                     GHLVAGLSGAQGSGKSTLAAVLKGLLDGWGLPTAVLSIDDLYLTHAERLRLGERVHPL
                     LRTRGVPGTHDVALGLATLERLRCVGPLALPRFDKQRDDRLPRAQWPMFDAPASVVIL
                     EGWCVGALPQSDAELSAPVNALERTEDPRGEWRGYVNDALRGAYRDLFGRLDMLLLLE
                     APSFDVVHGWRLEQEHKLRDRVTAAGDDASRVMDEAGVARFISHYERITRHILREMPG
                     RADIVLELDAHRAPLRLSGQMS"
     gene            1237..1680
                     /locus_tag="PLW13_06170"
     CDS             1237..1680
                     /locus_tag="PLW13_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQY69993.1"
                     /translation="MQLTTRGRYAVTAMLDLALHAGEGTMRLSDIAERQGLSLSYLEQ
                     LFARLRREGLVASVRGPGGGYRLAQPHGELSIGKIIDAVNEHLDVTRCKGKGDCQQGD
                     TCLTHHLWTELSDNLHQFLHRITIADLLRDHAARQHGAGHTEGPP"
     gene            1677..3113
                     /gene="sufB"
                     /locus_tag="PLW13_06175"
     CDS             1677..3113
                     /gene="sufB"
                     /locus_tag="PLW13_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQY69994.1"
                     /translation="MTEQIQSLLKQDYEAGFVTRVDSDTFAPGLDEEVVRRISARKGE
                     PDWLLEWRLKAYRAWVTMHEPTWAHVRFDPIDYNAVSYYSAPKSMADGPKSLEEVDPE
                     LLKTYEKLGIPLHERARLAGVAVDAVFDSVSVVTTFKDKLHEAGVVFCPISEAVHSHP
                     ELVQKYMGSVVPQRDNFYAALNSAVFSDGSFVYIPPGVRCPMELSTYFRINEAKTGQF
                     ERTLIIADEGSHVSYLEGCTAPMRDENQLHAAVVELVALKGARIKYSTVQNWYPGDRE
                     GKGGIYNFVTKRGVCHADASISWTQVETGSAITWKYPSVILRGDNSVGEFYSVALTNN
                     LQQADTGTKMIHLGKNTRSTIISKGISAGRSSNAYRGLVRINPGAEGARNYTQCDSLL
                     IGDKCGAHTFPYIESRNPSATVEHEATTSKVSEDQLFLCQQRGLDAEKAVSMIVNGFC
                     REVFKELPMEFAVEAGKLLEVSLEGSVG"
     gene            3322..4071
                     /gene="sufC"
                     /locus_tag="PLW13_06180"
     CDS             3322..4071
                     /gene="sufC"
                     /locus_tag="PLW13_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQY69995.1"
                     /translation="MLSISNLSARIDDKQILRGIDLEVKPGEVHAIMGPNGSGKSTLG
                     YVLSGREGYEVTGGSVRFEGKDLLAMATEERARAGLFLAFQYPVEIPGVSNMEFLKAS
                     VDALRSHRGQEALDAVRFMKLAREKCAAVDLKPDFLKRGVNEGFSGGEKKRNELLQMM
                     LLEPSLAILDETDSGLDIDALQVVANGVNSLRSPERSFVIVTHYQRLLDYIVPDFVHV
                     LAHGRIVKSGGKELALELEEKGYAWIEKEVA"
     gene            4072..5385
                     /gene="sufD"
                     /locus_tag="PLW13_06185"
     CDS             4072..5385
                     /gene="sufD"
                     /locus_tag="PLW13_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQY69996.1"
                     /translation="MGAFDQNLAARLFGDAEALPWLAAQREAARAQWLGASLPTRKTE
                     SWKYTSLESLNQLDYLNRPAPAAAPIDAAALIAGLDADRLVFVNGRYAPGLSQLGHQP
                     GVRAVSFREANPGDRAVIEGALGQIAAGSGNPFAALNGCWLEDGVLFHVGAGVKAARP
                     LHVVHLGVAQPQAFAHAQRLLVVLGRGAEAGLIEHFASLPGAHNGFVSGSTEIRVGEA
                     AQLRHYRLHLEEEHAIHIGGVYVELGRAALCESLVLALGARLKRLDLRVRHLGAGSEC
                     RLNGVYLARREQHVDLHTSVEHEVAQGTTHQVYRGIVDDAARAVFNGRIHIHPQAQKS
                     NADLSNRNLLLSNAAEVNTKPELEIYADDVRCSHGATVSRIDEKSLYYLLTRGIGRRE
                     AEVLLGFGFINELVRDIPIEPLGVTLRAVLSDWFGRADELQGEPS"
     gene            5382..6626
                     /locus_tag="PLW13_06190"
     CDS             5382..6626
                     /locus_tag="PLW13_06190"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQY69997.1"
                     /translation="MTPARAPAVAAFDAGRLRAEFPILAQQVNGKPLVYLDNAATTQK
                     PVVVIEAIASYYREINSNVHRGAHALSDRATSAFEGARDAVCRFINAPSREQVIWTRG
                     TTESINLVAATFARSRLRAGDEILVSGLAHHSNIVPWQLAAEAAGAKVVPIPVTDDAR
                     LDLEAFARLLGERTRLVALEHVSNAMGTVHPVERIIAMAHAAGVPVLLDGAQAVGHMP
                     VDVQALDVDFYAFSGHKMFGPTGIGVLYGKREWLESMPPWQGGGEMIETVSFDDTRYA
                     ALPYRFEAGTPHIAGAVGLGAAIEWLAGIDRAAATAHEDALLAHALELAQELPGFRLV
                     GSAPHKVAVLSFLLEGAHPQDVGTLLDQQGVAVRTGHHCAQPVMARFGVPGTIRASFS
                     LYNTHAEVEGLFAALHKVRSFL"
     gene            6623..7003
                     /locus_tag="PLW13_06195"
     CDS             6623..7003
                     /locus_tag="PLW13_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQY69998.1"
                     /translation="MSTAIEVQHADPQAPVVVTEAAVRHFRQQIQAAKAAGLRLSIKE
                     SGCTGYMYVMDLVETPPADDLLMQLADDVRLWIDRASLPLLRGTTVDFVREGLNSLLR
                     FGNPNAKDYCGCGESFSIEPEVQA"
     gene            7003..7536
                     /gene="sufT"
                     /locus_tag="PLW13_06200"
     CDS             7003..7536
                     /gene="sufT"
                     /locus_tag="PLW13_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HQY69999.1"
                     /translation="MERRMVVTQRDCPARLVPVGTPITIPKDSFVTITQALGGSYTVT
                     VNGNMARVDGTDADALGFAALELDFAAPADGCIAEEQVYEALRTVFDPEIPVDIVALG
                     LVYSVDIDQQAHAVRIRMTLTAPGCGMGPVLVGDVEYRVAKVPHVRQVTVDLVFDPPW
                     SREMMSDEAKLETGLFY"
     gene            7548..7973
                     /locus_tag="PLW13_06205"
     CDS             7548..7973
                     /locus_tag="PLW13_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQY70000.1"
                     /translation="MDARTTHSVEDIVETIGFFDDWEDRYRYIIDLGKELPAMPDARK
                     REELLVRGCQSQVWLDWTEREGRIFFDVDSDALIVRGLIAIMLAAFDGRTPRAILDFD
                     IEGYFERLDLLKHLSPTRGNGLRAMVQRVRDIAAGLAKR"
     gene            8027..8935
                     /locus_tag="PLW13_06210"
     CDS             8027..8935
                     /locus_tag="PLW13_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY70001.1"
                     /translation="MRQDVEFPGFGGTTLRGWLYTPERAGNGPALVMAHGFSATRAMA
                     LDPFARVFREAGITVLVYDHRNLGASDGEPRQEINIWAQARDYRYAIDWLATRPHVDS
                     ARIGIWGSSFSGGEVLAVGACDRRVAAVIANVPFASLPDTEQRLADSTAFDRMRAALL
                     DESGSGPADAASEAMGPLAVVPEEGVELPAFLAFQEASEWFLRAGAEPGSGWHNRVTL
                     RNCFGTEPPFDPGVCVSHIAPTPLLMVVASDDRLAPTSGALKAFERAGEPKRLELIEG
                     DHFVAYQGEGFARAAPVMREFLLEHL"
     gene            complement(8937..10103)
                     /locus_tag="PLW13_06215"
     CDS             complement(8937..10103)
                     /locus_tag="PLW13_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HQY70002.1"
                     /translation="MSELFVAVSPERVIFAKNSDSDPNEAQVLGWIAAREHAADATLR
                     CTWRAIPQVRRTHALMLSRPFWMWGAEMGVNEHGVAIGNAAVFARATREPDGLPGADL
                     VRLGLERGASAAQAIEVIAALLSEHGQGAGGHHSFLVADRREAWLLECEGRDSARERI
                     SAGVRAIAGAPALPALLPRSRRLQAWISGAGARRARIETLAAGVGDAATAAALLSDHG
                     GAAPRYNRCSGALHAPCVHAGGWLAASQTTASWISELAGGGDRHWATGTAAPCLGLFR
                     PLALRRARNGGHPSGSPDLDSLWWRFERLHRHVLRDPGALPPSFHADRARTQRAIFED
                     PVAGWDIAEDWLARWEALAGTPRADARPRWLRRYWEHIEAQAVQGSALPWREAP"
     gene            10221..10616
                     /locus_tag="PLW13_06220"
     CDS             10221..10616
                     /locus_tag="PLW13_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQY70003.1"
                     /translation="MPIEAYHWSTAVTLLALLVYFSVTAKTGQARLKYGVKAPATSGA
                     PEFERAFRIQQNTLEQLMLFLPSLWLFAALVSDLWAGAAGLVWVIGRILYAVGYARAA
                     EKRSAGFGIAILANLVLLIGALVGWFLRG"
     gene            10624..11418
                     /locus_tag="PLW13_06225"
     CDS             10624..11418
                     /locus_tag="PLW13_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY70004.1"
                     /translation="MNSFGADTAVERLQPGSYRGALASGWDIGGNANGGYMLAIAARA
                     MATETGRPDPVTITAHYLSPGRAGPVTVDVRVVKQGRRFGVASAAMCAAGQPLLQVLG
                     TFGNLERSDAGLERIESAPPELPPPEECFGHRVENGAPALFGNVDLRLHPEDAGFASG
                     KPSGQLRVRGWFRFGRDEPIDTLGLLLAVDSFPPTSFNANLPVAWTPTIELTTHLRAR
                     PAPGWLRASFSSRFLSGGFVEEDGEVWDSTGRLVAQSRQLALLPLN"
     gene            11440..12546
                     /locus_tag="PLW13_06230"
     CDS             11440..12546
                     /locus_tag="PLW13_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HQY70005.1"
                     /translation="MKTRAAVAFAAGQPLEIVEVDLEGPKAGEVLVEIKATGVCHTDA
                     FTLSGDDPEGAFPAILGHEGAGVVVEVGPGVKSVAVGDHVIPLYTPECRECDYCLHPK
                     TNLCQAIRSTQGQGLMPDGSSRFSYKGQKILHYMGCSTFSNYTVLPEIAVAKIRKDAP
                     FDKVCYIGCGVTTGVGAVAFTMKVEPGSTVAVFGLGGIGLNVIQGARMVGATRIIGVD
                     LNPAKVELARRFGMTDFVNPSEVKDVTQAIIDMTNGGVDYSFECIGNVKVMRQALECC
                     HKGWGQSCIIGVAGAGQEISTRPFQLVTGRAWRGSAFGGARGRTDVPRIVDWYMDGRI
                     AIDELITHTMPLEDINKAFDLMHAGESIRSVVMY"
     gene            12558..13403
                     /gene="fghA"
                     /locus_tag="PLW13_06235"
     CDS             12558..13403
                     /gene="fghA"
                     /locus_tag="PLW13_06235"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HQY70006.1"
                     /translation="MEQIGSHRCFGGQQLRFTHRSETLDCDMAFSLYLPPQAEYAPVP
                     LLHWLSGLTCTDENFVIKAGAQRYAARHGIAILAPDTSPRGELVPGDPEGGWDLGLGA
                     GFYVDATREPWRGHYRMYDYVSRELPALVNAKFPVLAERQAISGHSMGGHGALTIALK
                     NPGQFRSVSAFAPICSPTRCPWGEKAFTHYFGEERAAWEAHDASLLLAKAAEQLPTLV
                     DQGEADGFLGAQLKPELLVQAAQAAGYPLRLRMQPGYDHSYFFIASFIGEHIAFHSEH
                     LHDAP"
     gene            13425..14243
                     /gene="fdhD"
                     /locus_tag="PLW13_06240"
     CDS             13425..14243
                     /gene="fdhD"
                     /locus_tag="PLW13_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQY70007.1"
                     /translation="MSAAQCREELPVTRHGDSRQGPESATDPIALETPVALSYNGLSH
                     VVMMMTPADLEDFALGFSLSEGIVERPGQIYDIATKTHPQGLEIAITLGNERLQQLKD
                     RRRNLAGRTGCGLCGAESLEQALRPVPRLACDTVIATGAIQRALARLDEWQPLQRATG
                     AAHGAAWCSGDGGIVLAREDVGRHNALDKLIGALARAPAARGEGFALVSSRASYEMVS
                     KAAQAGIGILVAVSAPTALAVRLAAEAGLTLAAFARAGKVSVYTRPERLAPCET"
     gene            14231..14905
                     /locus_tag="PLW13_06245"
     CDS             14231..14905
                     /locus_tag="PLW13_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQY70008.1"
                     /translation="MRNLIRNFQSYWLADASFLTLLIMLIAAVFVLPVIMEISGHGVL
                     LFNILLLSVFFSGIFSTRSVTLISLSAILFSIHLTLRIIRFGENPYSFYVLENVIGIA
                     NGLIFIFINLRLLFRDQIVNTYRIVGAVNVYLLLALVGALALEVIHAATGVSIGGNVV
                     LSGKDGDYVHFIYFSLASLTTVGFGDIYAVNTFARMLATFLSTLGVLFPAIVIARLVG
                     LASSRS"
     gene            14919..17342
                     /locus_tag="PLW13_06250"
     CDS             14919..17342
                     /locus_tag="PLW13_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70009.1"
                     /translation="MPRLLPPKRLLFRACLLGAAFAVAPLAASAAGKIGIIGDSMAAG
                     THSLDNCGSLDIVECVEERGGYQDRAWSYARGAHAWPLAARLGFTPAQVVDAADDGEE
                     WKDAYDQAAQVLSDPALDTVTIGLGANDICQPSGHDYAGDLERVAGHIDRTLQLLTDR
                     LPEGGTIYWSAVPDVAGLYDMLRERDHNLLFESCQATWDLDTRKVSDGAAQDACDHFF
                     DNDLCRFADTQEQAKDRLVSLFLDRLLASGGVEEGPCGKILSSRSTDEDRREAREFTA
                     ALNGLMREKADAFDGRNGVRVRYSEQVYRPTRPLAPEHVSHFDCYHPSRAGQVFLADQ
                     IWRGFEGIRPQGHDVFVEDFGAPQYCRADAAAWNGCWIEANDDDEPASGNVRIGDGRL
                     QVGGAGTSLERGFALGGYSHAWLSFNWARDDLDRALDYVSVEVSADGGASWAEVARFA
                     GDGNDFGLHRGDYHDLSDYASADTRIRFRASPGLGSGDRVLFDNVRLIAWNEVPFTAW
                     PEVPATGSVQSGVGLIRGWACAAETVSVSIDGGDPIAVAWGGQRDDTAGVCGDAANGY
                     GMVLPWGLLGNGPHRMQTFIDEAQVGNAEFEVSGLGEEFVEGLQGEYVLAGFPAAGER
                     VRLTWSEADQNFLITGHDTGAGFTDPSATGIRADAAARAGGAHHESPAQHSIQSGVGL
                     IRGWACDAEEVTISIDGGAPIPVAHGTSRADTIGVCGDEDNGYGMVFAWGLLGTGVHR
                     LQTFVDGVEIADVEFAVQAIGDGFVEGLQGRYTLDGFPGPGQAVDVQWSEPDQNFRII
                     AVQPDAAGD"
     gene            17404..>18041
                     /locus_tag="PLW13_06255"
     CDS             17404..>18041
                     /locus_tag="PLW13_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HQY70010.1"
                     /translation="MNTRSLLEQLLQSGSKYLGNQPGVANKSQGGSQDPLSGFLSGAA
                     GGALAGSAMSLLLGSKKARKATGNLAMYGGLAALGVVAYKAYDNWQKTQAGMPQSQPQ
                     TVDRLSGTQAEEHSQAILRALIGAAKADGHIDARERALIDGEIAKLGADPELARWFDQ
                     ELGKPLDPAEVAAASRSTEMAAEMYLASVLIVDEDSFMERAYLQELGRQLKLA"
BASE COUNT         2829 a         6081 c         6342 g         2789 t
ORIGIN      
        1 cgccggagag cgtggcgcgg ctgaaccagt tccgcgactg catcatccgc gtgcacgatc
       61 acagcaccgg ggcgacgggg tgggggaatt gccagaccta cctggtcggg gattcatgac
      121 atctgtccgg ataatcgcag cggcgcgcgg tgcgcgtcga gttccaggac gatgtccgcc
      181 cgcccgggca tctcgcggag aatgtgccgg gtgatgcgct cgtagtggct gatgaagcgc
      241 gcgacaccgg cctcgtccat caccctcgat gcatcgtccc cggcggctgt cacccggtcg
      301 cgcaatttgt gttcctgctc caggcgccag ccatggacca cgtcgaacga cggcgcctcg
      361 agtaacagca gcatgtcgag acgcccgaac aggtcgcggt acgcgccgcg cagcgcatcg
      421 ttcacatagc cgcgccattc gccgcgcggg tcctcggtgc gctccagtgc gttcaccggc
      481 gccgacagct ccgcatcgct ctgcggcagt gcgccaacgc accagccttc gaggatcacc
      541 accgacgcgg gcgcgtcgaa catcggccac tgtgcccggg gcaggcgatc atcgcgctgc
      601 ttgtcgaagc ggggcagggc gagcggcccc acgcaacgca gccgctccag tgtcgcaagt
      661 cccagcgcca cgtcatgcgt gcccgggacg ccgcgtgtgc gcagcagcgg gtgcacgcgt
      721 tcgcccagcc gcagacgttc cgcgtgcgtc aggtacaggt cgtcgatcga gagcaccgcg
      781 gtaggcagtc cccatccgtc gagcagcccc ttcagcaccg ccgccagcgt cgatttgccg
      841 ctgccctgcg cgccgctcag gcccgcaaca aggtggccgc cccgacgccg cgcctcggtg
      901 gccacgcgcg ccgcgatcgg cgcatgcacc tcggcgatca gcgtacggaa ctgtgacggc
      961 agccgctcgc gcgagaggaa ggcgtcgatc caggcggtgg tgacgggcat cggggctcat
     1021 tgccagggcg tagcaagtcg gtgcagatcg accggcgcgt gaggtccggc cactgcgtga
     1081 cggcagggta gcccggatcg gtggcggcgc gcagcagggc gagtcccgga ctgtcctata
     1141 cccgagtttt ttactgcact aaaaagttga ctgtcttagt ggggtattgg atactgcagc
     1201 cgtgtttccg gccacctcgc agaggctcag aatccgttgc aactcaccac ccgtggacgc
     1261 tacgcagtga ctgccatgct cgatctcgcg ctgcacgcgg gcgagggcac gatgcggctg
     1321 tccgacatcg ccgagcgcca gggcctgtcg ctgtcctacc tcgagcagct gttcgcgcgg
     1381 ctgcgccgcg aggggctggt ggccagtgtg cgcgggcccg ggggcggcta tcgcctggca
     1441 cagccgcacg gggagctgag catcggcaag atcatcgacg cggtgaacga gcacctcgac
     1501 gtgacccgct gcaagggcaa gggcgattgc cagcagggcg acacctgcct gacgcaccat
     1561 ctgtggaccg aactgagcga taatctgcac cagttcctgc accgcatcac cattgccgac
     1621 ctgttgcgcg accatgccgc gcgccagcac ggcgccgggc acacggaggg cccgccatga
     1681 ccgagcagat ccagagcctg ctgaagcagg actacgaagc gggtttcgtg acccgcgtcg
     1741 actccgacac ctttgcgccg ggcctcgatg aagaggtcgt gcggcggatt tccgcgcgca
     1801 agggcgagcc cgattggctg ctcgaatggc gcctcaaggc ctatcgcgcc tgggtgacga
     1861 tgcacgagcc cacgtgggcg cacgtgcgct tcgacccgat cgactacaac gcggtgtcct
     1921 attactcggc gcccaaatcc atggccgacg gcccgaagag tctcgaggag gtcgaccccg
     1981 agctgctgaa gacctacgag aagctcggca tcccgctgca cgagcgcgcc aggcttgccg
     2041 gcgtggctgt cgacgcggtg ttcgactcgg tctcggtggt caccaccttc aaggacaagc
     2101 tgcacgaggc cggcgtcgtg ttctgtccga tctcggaggc cgtgcactcg caccccgagc
     2161 tggtgcaaaa gtacatgggc agcgtggtgc cgcagcgcga caatttctac gcggcgctga
     2221 attccgcggt cttcagcgac ggctccttcg tctacatccc gccgggcgtg cgctgcccga
     2281 tggagctctc gacctatttc cgcatcaacg aggcgaaaac cggccagttc gagcgcacgc
     2341 tgatcatcgc cgacgagggc agccacgtga gctacctcga gggctgcacg gcgccgatgc
     2401 gcgacgagaa ccagctgcac gccgcggtgg tcgagctggt cgcgctgaag ggcgcgcgca
     2461 tcaagtactc cacggtgcag aactggtacc ccggggaccg cgagggcaag ggcggcatct
     2521 acaacttcgt gaccaagcgc ggcgtgtgtc atgccgacgc ctcgatctcg tggacgcagg
     2581 tcgagaccgg ctcggcgatc acctggaaat acccttccgt gatcctgcgc ggcgacaact
     2641 cggtaggcga gttctactcg gtggcgctca cgaacaacct gcagcaggcc gacaccggca
     2701 ccaagatgat ccacctcggg aagaacacgc gctcgacgat catctccaag ggcatctcgg
     2761 cgggccgctc cagcaacgcc taccgcgggc tggtgcgcat caatccgggc gccgagggcg
     2821 cgcgtaacta cacgcagtgc gactcgctgc tgatcggcga caagtgcggc gcgcacacct
     2881 tcccgtacat cgagagccgc aacccctcgg ccacggtcga gcacgaggcc acgacctcga
     2941 aggtcagcga ggaccagctg ttcctgtgcc agcagcgcgg cctggatgcg gagaaggcgg
     3001 tgtcgatgat cgtcaatggc ttctgccgcg aggtgttcaa ggaactgccg atggagttcg
     3061 ccgtggaagc cggcaagctg ctcgaggtca gcctcgaggg ttcggtcggt tgagcggagt
     3121 gccgccggct ctgcgcgggg cggcgctgcg ccgcgtacgg cgccgcgaca cgccgtgaat
     3181 acgtccctgt aggctcgact gcggcatcca tgccgcagac ggtcgccgcg ccatacgcgg
     3241 cacagcgccg cctggcgcgc ggtggaacgc aagtttatac gccacccgga attggatggg
     3301 tggtgagaat caggagtgac aatgctgtcc atatcgaatc tgagcgcgcg catcgacgac
     3361 aagcagatcc tgcgcggcat cgacctcgag gtgaagccgg gcgaggtgca cgcgatcatg
     3421 gggcccaacg gctcgggcaa gagcacgctg ggctacgtgc tgtcggggcg cgagggctac
     3481 gaggtcaccg ggggcagcgt gcgcttcgag ggcaaggacc tgctcgcgat ggcgaccgag
     3541 gagcgcgcgc gcgccgggtt gttcctcgcg ttccagtacc cggtcgagat cccgggcgtg
     3601 agcaacatgg aattcctcaa ggcctcggtg gatgcgctgc gttcgcatcg cggccaggag
     3661 gcgctggacg cggtgcgctt catgaagctc gcgcgcgaga aatgcgccgc ggtggacctg
     3721 aagcccgatt tcctgaagcg cggcgtcaac gaaggtttct cgggcggcga gaagaagcgc
     3781 aacgagctgc tgcagatgat gctgctcgag cctagcctcg ccatcctcga cgagaccgac
     3841 tccgggctcg acatcgacgc gctgcaggtg gtggccaacg gcgtgaacag cctgcgctcg
     3901 ccggagcgtt ccttcgtgat cgtgacgcat taccagcgcc tgctcgatta catcgtgccg
     3961 gatttcgtgc acgtgctcgc gcacgggcgc atcgtgaagt ccggcggcaa ggaactcgcg
     4021 ctcgagctcg aggagaaggg ctacgcctgg atcgagaaag aggtcgcgta gatgggcgcc
     4081 ttcgaccaga acctggccgc gcgcctgttc ggcgacgccg aggcgctgcc ctggctcgcg
     4141 gcgcagcgag aggcggcgcg cgcgcaatgg ctgggcgcga gcctgcccac gcgcaagacc
     4201 gagtcgtgga aatacaccag cctcgaatcg ctgaaccagc tcgactacct gaaccggccc
     4261 gcaccggccg cagcgcccat cgatgccgcg gcgcttatcg cggggctcga cgcggaccgg
     4321 ttggtgttcg tgaacggtcg ctacgcgccg gggctgtcac agctcgggca ccagccgggc
     4381 gtgcgcgcgg tctcgttccg cgaagccaac ccgggggatc gcgcggtgat cgagggcgcg
     4441 ctggggcaga tcgccgcggg cagcggcaat cccttcgcgg cgctgaacgg ctgctggctc
     4501 gaggacggcg tgctgttcca cgtcggtgcc ggtgtgaagg ccgcgcggcc gctgcacgtg
     4561 gtgcacctcg gcgtggcgca gccgcaggct ttcgcccatg cgcagcgcct gctggtggtg
     4621 ctcgggcgcg gcgccgaagc cgggttgatc gagcatttcg cgagcctgcc gggcgcgcac
     4681 aacggcttcg tcagcggcag caccgagatc cgcgtgggcg aggcggcgca gttgcgccat
     4741 taccgcctgc acctcgagga ggagcacgcg atccatatcg gcggcgtcta cgtcgagctc
     4801 gggcgcgccg cgctctgcga gagcctggtg ctggcgctcg gcgcacgcct caagcgtctg
     4861 gacctgcgcg tgcgccatct cggtgccggc agcgaatgcc gcctgaacgg cgtctatctc
     4921 gcgcgccgcg agcagcacgt cgacctgcac acctcggtcg agcacgaggt ggcgcagggc
     4981 accacgcacc aggtctatcg cggcatcgtc gacgatgccg cgcgcgcggt gttcaacggg
     5041 cgcatccaca ttcatccgca ggcgcagaag agcaacgccg atctcagcaa ccgcaacctg
     5101 ctgctcagca acgcggccga ggtcaacacc aagccggaac tggagatcta cgccgatgac
     5161 gtgcgctgct cgcacggcgc cacggtgagc cgcatcgacg agaagagcct gtattacctg
     5221 ctgacgcgcg gcatcgggcg ccgggaagcg gaagtgctgc tcggcttcgg cttcatcaac
     5281 gagctggtca gggacatccc gatcgagccg ctcggcgtga cgctgcgcgc cgtgcttagc
     5341 gactggttcg ggcgcgctga cgaactgcag ggagagccct cgtgacgccg gcccgtgcac
     5401 ccgccgttgc ggccttcgat gccgggcgcc tgcgtgccga gttcccgatc ctcgcccagc
     5461 aggtcaacgg caagccgctg gtgtacctcg acaacgccgc gaccacgcag aagcccgtgg
     5521 tcgtgatcga ggcgatcgcg tcctactacc gcgagatcaa ttccaacgtg catcgcggcg
     5581 cgcacgcgct gagcgatcgc gccaccagcg ccttcgaggg cgcgcgcgac gcggtgtgcc
     5641 gcttcatcaa cgcgccctcg cgcgagcagg tgatctggac ccgcggcacc accgagagca
     5701 tcaacctggt cgccgcgacc ttcgccagga gccgcctgcg cgcgggcgac gagatcctgg
     5761 tctcggggct ggcgcaccac tcgaacatcg tgccgtggca gctcgcggcc gaggccgccg
     5821 gcgcgaaagt cgtgccgatc ccggtcaccg atgacgcgcg gctcgatctc gaggccttcg
     5881 cgcgcctgct cggcgagcgc acgcggctgg tggcgctgga gcacgtctcg aacgcgatgg
     5941 gcacggtgca tccggtcgag cggatcatcg ccatggcgca cgccgccggc gtgccggtgc
     6001 tgctcgacgg cgcgcaggcc gtgggccaca tgccggtcga cgtgcaggcg ctcgacgtgg
     6061 atttctacgc cttctcgggc cacaagatgt tcggccccac gggcatcggc gtgctctacg
     6121 gcaagcgcga gtggctggaa tcgatgccgc cctggcaggg cggtggcgag atgatcgaga
     6181 ccgtgtcctt cgacgacacg cgctacgccg cgctgcccta ccgcttcgag gccggcacac
     6241 cgcacatcgc cggcgcggtg gggctggggg cggcgatcga atggctcgcg ggcatcgacc
     6301 gcgccgccgc caccgcgcac gaggacgcgc tgctcgcgca cgcgctggag ctcgcgcagg
     6361 aactgccggg cttccgtctc gtcggcagcg cgccgcacaa ggtcgcggtg ctcagcttcc
     6421 tgctcgaggg cgctcatccg caggacgtgg gcacgctgct cgaccagcag ggtgtcgcgg
     6481 tgcgcaccgg gcaccattgc gcgcaaccgg tgatggcacg cttcggcgtg cccggcacga
     6541 tccgtgcgtc cttcagtctc tacaacacgc acgccgaggt cgaagggctg tttgcggcgt
     6601 tgcacaaggt acggagtttc ctgtgagcac tgccatcgaa gtccagcatg ccgatccgca
     6661 ggcgccggtc gtggtgaccg aggccgccgt gcggcatttc cgccagcaga tccaggctgc
     6721 gaaggccgcc ggcttgcgcc tcagtatcaa ggaaagcggc tgcaccggtt atatgtacgt
     6781 gatggacctg gtcgagacgc cgccggccga cgacctgctg atgcagctcg cggacgatgt
     6841 gcggctgtgg atcgaccgcg cctcgctgcc gttgctgcgc ggcaccacgg tcgatttcgt
     6901 gcgcgagggg ctgaacagcc tgctgcgctt cggcaacccg aacgcgaagg actactgcgg
     6961 ctgcggcgag agcttcagca tcgagccgga ggtgcaggcc tgatggagcg gcgcatggtg
     7021 gtgacgcagc gcgactgtcc tgcgcgcctg gtgccggtgg gcacgccgat cacgatcccg
     7081 aaggactcct tcgtcacgat cacgcaggcg ctgggcggca gctacacggt gacggtcaac
     7141 ggcaacatgg cgcgcgtgga cggcaccgat gccgacgcgc tgggcttcgc ggcgctggag
     7201 ctggatttcg cggcgcccgc cgacgggtgc atcgcggagg agcaggtcta cgaggcgctg
     7261 cgcacggtgt tcgacccgga aatcccggtg gacatcgtcg cgctggggct ggtgtattcc
     7321 gtggacatcg accagcaagc gcacgccgtg cgcatccgca tgacgctgac cgcgccgggt
     7381 tgcggcatgg gtccggtgct ggtcggcgac gtcgaatacc gcgtggcgaa ggtgccgcac
     7441 gtgcgccagg tcacggtgga cctggtgttc gacccgccgt ggtcgcgcga gatgatgtcc
     7501 gacgaggcga agctcgagac ggggttgttc tattgatgga ggcgaatgtg gacgcccgaa
     7561 caacgcacag cgtcgaggac atcgtcgaga cgatcggttt cttcgacgac tgggaagacc
     7621 gctaccgcta catcatcgat ctgggcaagg agctgccggc gatgcccgat gcgcgcaagc
     7681 gcgaggaact gctggtgcgc ggctgccaga gccaggtgtg gctggactgg accgagcgcg
     7741 aggggcgcat cttcttcgat gtcgacagcg atgcgctgat cgtgcgcggc ctgatcgcga
     7801 tcatgctcgc cgctttcgac ggccgcacgc cgcgcgcgat cctcgacttc gatatcgagg
     7861 gctatttcga acgactcgac ctgctgaagc acctgagccc cacgcgcggc aacggcctgc
     7921 gcgcgatggt gcagcgcgtg cgcgatatcg ccgcgggcct ggcgaagcgg tgataatcgg
     7981 cgcgcgggcg gcgaacggcg ccggccccgt cttccaggag cacggcatgc gacaagacgt
     8041 tgaattcccc ggcttcggcg gcaccacgct gcgcggctgg ctctacactc ccgaaagggc
     8101 cggcaacggc ccggcactgg tgatggcaca cggcttctcg gcgacgcgcg cgatggcgct
     8161 cgacccgttc gcgcgcgtgt tccgcgaggc gggcatcacg gtgctcgtct acgatcaccg
     8221 caacctgggt gcgagcgatg gcgagccgcg gcaggaaatc aacatctggg cgcaggcgcg
     8281 ggactaccgc tacgcgatcg actggctggc cacgcgcccg cacgtcgatt ccgcgcgcat
     8341 cggcatctgg ggttccagct tcagcggcgg cgaggtgctg gcggtgggag cctgcgaccg
     8401 gcgcgtggcg gcggtcatcg ccaacgtgcc cttcgccagc ctgccggata ccgagcagcg
     8461 cctcgccgac agcaccgcgt tcgatcgcat gcgcgccgca ctgctggacg agagcggcag
     8521 cgggccggcc gatgctgcga gcgaggcaat gggtccgctg gcggtggtgc cggaggaggg
     8581 cgtggagctg ccggctttcc tggcctttca ggaagcgagc gagtggttcc tgcgcgcggg
     8641 cgccgagccg ggcagcggct ggcacaaccg cgtgacgctg cgcaactgtt ttggcaccga
     8701 gccgcctttc gatcccggtg tctgcgtgag ccatatcgcg cccacgcccc tgctgatggt
     8761 ggtcgcgagc gacgatcgcc tggcgcccac ctcaggcgcg ctgaaggcct tcgagcgtgc
     8821 cggcgagccc aagcgcctgg agctgatcga gggcgaccat ttcgtggcct accagggcga
     8881 aggattcgcg cgtgcggcgc cggtgatgcg ggaattcctg ctcgagcatc tctgagtcac
     8941 ggggcttcgc gccagggcag tgcgctgcct tgcacggcct gcgcctcgat gtgctcccag
     9001 tagcgcctga gccagcgcgg gcgcgcgtcg gcgcgcggcg tacccgccag agcctcccag
     9061 cgcgccagcc agtcctcggc gatgtcccag cccgcgaccg gatcctcgaa gatcgcgcgc
     9121 tgcgtgcgcg cgcgatcggc gtggaacgag ggcggcaggg cgccggggtc gcgcagcacg
     9181 tggcggtgca gccgctcgaa gcgccaccac aggctgtcga gatcgggact gccgctcgga
     9241 tgtccgccgt tgcgtgcgcg gcgcaacgcg agcgggcgga acaggccgag gcagggcgcg
     9301 gcggtgccgg tggcccagtg gcgatcgccg cccccggcga gttcgctgat ccagctcgcg
     9361 gtggtctggc tcgccgcgag ccagccgccc gcgtgcacgc agggggcgtg cagcgccccg
     9421 ctgcaacggt tgtagcgtgg cgcggcgccg ccgtggtcgc tgagcagcgc cgcggcagtc
     9481 gcggcatcgc ccacgccggc agccagcgtt tcgatgcgtg cgcgtcgcgc gccggcaccg
     9541 ctgatccagg cctgcagccg gcgcgaacgc ggcagcagcg cgggcaaggc tggcgcgccc
     9601 gcgatggcgc gcacgccggc acttatgcgt tcgcgcgcgc tgtcgcgtcc ctcgcattcc
     9661 agcagccatg cttcgcgccg gtcggcgacc aggaagctgt ggtggccacc ggcgccctgg
     9721 ccgtgctcgg acagcagcgc ggcgatcacc tcgatggctt gcgccgcgct ggcgccgcgc
     9781 tcgagcccca gtcgcaccag gtccgcgccc ggcaggccgt cgggttcgcg cgtggcgcgc
     9841 gcgaacaccg cggcgttgcc gatggcgact ccgtgctcgt tgacgcccat ctccgcgccc
     9901 cacatccaga acggccggct gagcatcagc gcgtgcgtgc ggcgcacctg cgggatcgcg
     9961 cgccaggtgc agcgcagcgt ggcgtcggcg gcatgctcgc gcgccgcgat ccagccgagc
    10021 acctgcgcct cgttggggtc gctgtcgctg tttttcgcga agatcacgcg ctcggggctg
    10081 acggccacga agagctcgga catggcggtg gtccgtgatt gcgggtgtgc cggcaaagat
    10141 aggcaagccg tggagacatt gcacggtgaa ggacttagaa tccggtgcga acgccaatcc
    10201 catcacaagg agcacgaacg atgccgatcg aagcctacca ctggagcacc gcggtcacgc
    10261 tgctcgcgct gctggtgtat ttctcggtca ccgccaagac gggccaggca cgcctcaagt
    10321 atggcgtgaa agccccggcc accagcggcg cgcccgagtt cgagcgggcg ttccgcatcc
    10381 agcagaacac gctggagcag ctgatgctgt tcctgccctc gttgtggctg ttcgccgcgc
    10441 tggtgagcga tttgtgggcc ggcgcggcgg gcctggtgtg ggtgatcggg cgcattctct
    10501 acgccgtcgg ctatgcgcgc gcggcggaga agcgcagcgc cggtttcggc atcgcgattc
    10561 tcgccaacct ggtgctgctg atcggcgcgc tggtcggctg gttcctgcgc ggctgacacg
    10621 ggcatgaatt ccttcggcgc ggacaccgcc gtcgagcgcc tgcagccggg cagctatcgc
    10681 ggcgcgctcg cgagcggctg ggacatcggc ggcaacgcca acggcggcta catgctcgcg
    10741 atcgcggcgc gcgcgatggc gaccgagacc gggcgcccgg acccggtcac gatcaccgcg
    10801 cactacctct cgcccgggcg cgccggcccg gtcacggtgg acgtgcgcgt ggtgaagcag
    10861 ggccggcgct tcggcgtggc gagcgcggcg atgtgcgccg ccggacagcc cctgctgcag
    10921 gtgctgggca ccttcggcaa cctcgagcgc tccgatgcgg gcctcgagcg catcgagtcc
    10981 gcgccgcccg agctgccgcc gccggaagag tgcttcgggc accgcgtcga gaacggcgcc
    11041 ccggcgctgt tcggcaacgt cgacctgcgc ctgcatcccg aggatgccgg cttcgcgagc
    11101 ggcaagccca gcgggcaact gcgcgtgcgc ggctggttcc gcttcggcag ggacgagccc
    11161 atcgacaccc tcggcctgct gctcgcggtg gactcctttc cgccgacctc cttcaacgcg
    11221 aacctgccgg tggcgtggac gccgacgatc gagctgacca cgcacctgcg cgcgcggccc
    11281 gcgccgggat ggctgcgcgc gagcttcagc tcgcgtttcc tcagcggggg attcgtcgag
    11341 gaggacggcg aggtctggga ttcgaccggc cgcctggtgg cgcagtcgcg ccagctcgcg
    11401 ctgctgccat tgaattgaac gacaacggag agcaaacgca tgaaaacacg cgcagccgtc
    11461 gcattcgccg caggccagcc gctggagatc gtcgaggtgg atctcgaggg accgaaggcc
    11521 ggcgaggtgc tggtggagat caaggccacc ggtgtgtgcc acaccgacgc gttcacgctc
    11581 tcgggcgatg atcccgaagg cgctttcccc gcgatcctcg gccacgaggg cgccggcgtg
    11641 gtggtggaag tgggtcccgg cgtgaagtcg gttgccgtgg gcgatcacgt gatcccgctc
    11701 tacacgcccg aatgccgcga gtgcgactac tgcctgcacc cgaagacgaa tctctgccag
    11761 gcgatccgct ccacgcaggg ccagggcctg atgcccgacg gcagcagccg cttctcgtac
    11821 aaggggcaga agatcctgca ctacatgggt tgctcgacct tctcgaacta caccgtgctg
    11881 cccgagatcg ccgtggcgaa gatccgcaag gacgcgccct tcgacaaggt ctgctacatc
    11941 ggttgcggcg tgaccaccgg cgtgggcgcg gtggcgttca cgatgaaggt cgagcccggc
    12001 tccacggtcg cggtgttcgg cctcggcggc atcgggctca acgtgatcca gggcgcgagg
    12061 atggtgggtg cgacgcgcat catcggcgtt gacctgaacc cggccaaggt ggagctcgcg
    12121 cgccgcttcg gcatgaccga cttcgtgaac ccgagcgagg tgaaggacgt cacgcaggcg
    12181 atcatcgaca tgaccaacgg cggcgtcgac tacagcttcg agtgcatcgg caacgtcaag
    12241 gtgatgcgcc aggcgctgga gtgctgccac aagggctggg ggcagagctg catcatcggc
    12301 gtggccggcg cggggcagga gatctcgacg cgcccgttcc agctggtcac cgggcgcgcg
    12361 tggcgcggct cggccttcgg cggcgcgcgc gggcgcaccg acgtgcccag gattgtcgac
    12421 tggtacatgg acggtcgcat cgcgatcgac gagctgatca cgcacacgat gccgctggag
    12481 gacatcaaca aggccttcga cctgatgcac gcgggcgaga gcatccgctc cgtcgtgatg
    12541 tactgaggcc ggcgaccatg gaacagatcg gatcgcaccg ctgtttcggc ggccagcagc
    12601 tgcgcttcac gcatcgctcg gagacgctcg actgcgacat ggccttctcg ctgtacctgc
    12661 cgccgcaggc cgagtacgcg ccggtgccgc tgctgcactg gctctcgggg ctgacctgca
    12721 ccgacgagaa cttcgtgatc aaggcgggcg cgcagcgcta cgcggccagg cacggcatcg
    12781 cgatcctcgc gcccgacacc agtccgcgcg gcgagctggt gccgggcgat ccggaaggcg
    12841 gctgggatct gggcctcggc gcgggcttct acgtcgatgc cacgcgcgag ccgtggcgcg
    12901 gccactaccg catgtacgac tacgtgagcc gcgagctgcc ggcgctggtc aacgcgaagt
    12961 tcccggtgct ggccgagcgc caggcgatca gcggccactc gatgggcgga cacggcgcgt
    13021 tgacgatcgc gctgaagaac cccgggcagt tccgcagcgt cagcgccttc gcgccgatct
    13081 gctcgccgac gcgctgcccc tggggcgaga aggcgttcac gcactacttc ggcgaggagc
    13141 gcgccgcctg ggaggcgcac gacgcctcgc tgctgctcgc gaaggccgcc gagcagctgc
    13201 cgacgctggt cgaccagggc gaggccgacg gcttcctcgg cgcgcaactc aagcccgagc
    13261 tgctggtgca ggcggcgcag gccgcgggct atccgctgcg cctgcgcatg caaccgggct
    13321 acgaccacag ctacttcttc atcgcctcgt tcatcggcga gcacatcgcg ttccattcgg
    13381 agcacctgca cgatgcgcct tgaacccccc ggttggctgc gcgcatgagc gcagcgcaat
    13441 gccgcgaaga gttgccggtc acgcgccatg gcgactcgcg gcagggtcct gaatccgcca
    13501 ccgacccgat cgcgctcgag acgcccgtcg cgctcagcta caacggcctc tcgcacgtcg
    13561 tgatgatgat gacgccggcg gacctcgagg atttcgcgct cggattcagc ctgtcggagg
    13621 gcatcgtcga gcgcccgggg cagatctacg acatcgcgac gaaaacgcac ccgcaggggc
    13681 tggagatcgc gatcacgctc ggcaacgagc gcttgcagca gctcaaggac cggcggcgca
    13741 acctggcggg gcgcaccggc tgcgggctgt gcggcgccga gtcgctggaa caggcgctgc
    13801 gcccggtgcc gaggctcgcg tgtgacacgg tgatcgccac gggcgcgatc cagcgcgcgc
    13861 tcgcgcgcct cgacgagtgg cagcccctgc agcgcgccac cggtgccgcg cacggtgcgg
    13921 cctggtgctc gggcgacggg ggcatcgtgc tcgcgcgcga ggacgtgggc cgccacaacg
    13981 cgctcgacaa gctgatcggc gcgctcgcgc gcgcgcccgc cgcgcgcggc gagggtttcg
    14041 cactggtatc cagccgcgcc agctacgaga tggtcagcaa ggcggcgcag gccggcatcg
    14101 gtatcctcgt cgccgtgtcg gcgccgaccg cgctcgcggt gcgcctcgcg gccgaggcgg
    14161 gcctcaccct ggcggccttc gcgcgcgccg ggaaggtgtc ggtctatacg cggcccgaaa
    14221 ggcttgcgcc atgcgaaacc tgatcaggaa tttccagtcc tactggctcg cggacgcgag
    14281 tttcctcacc ttgctgatca tgctcattgc cgcggtattc gtgctcccgg tcatcatgga
    14341 aatcagtggt cacggcgtac tgctgttcaa catactcctg ctcagcgtgt tcttttccgg
    14401 gatattctcg acccgcagcg tgacgctcat atccctctcc gcgatcctgt tcagcattca
    14461 cctgacgttg cggatcattc gtttcggaga gaacccgtat tctttctatg tcctggagaa
    14521 tgtcatcggc atcgccaacg ggctgatctt cattttcatc aacctgcggc tgctgtttcg
    14581 cgaccagatc gtgaacactt accggatcgt cggagcggtc aatgtctatc ttctgctggc
    14641 gctcgtgggg gcgctggccc tggaagtcat tcatgccgct accggcgttt ccattggcgg
    14701 caatgtcgtg ctgagcggga aagacggtga ctatgtccac ttcatctact tcagcctggc
    14761 ttcactgact accgtgggct tcggcgacat ctatgccgtg aataccttcg ccaggatgct
    14821 ggccaccttt ttatccacgc tgggcgtcct gtttccggcc atcgtcatcg cgcgcctggt
    14881 cggactggct tccagccgca gttgattgga gtcccctcat gcctcgtctg ctccccccga
    14941 agcgtttgtt gttccgtgcc tgcctgctgg gtgcggcgtt cgcggtcgct ccgctcgccg
    15001 cgtccgccgc cggcaagatc ggcatcatcg gcgacagcat ggcggccggc acgcacagcc
    15061 tggacaactg tggttcgctg gatatcgtcg aatgcgtcga ggagcgcggc ggataccagg
    15121 accgggcctg gagctatgcg cgcggcgcgc acgcgtggcc gctcgccgcg cggctgggtt
    15181 tcacgccggc acaggtcgtg gacgccgccg acgatggcga ggaatggaag gatgcctacg
    15241 accaggccgc gcaggtgctg tccgatcccg cgctcgatac cgtgacgatc gggctcggcg
    15301 ccaatgacat ctgccagccg agcgggcacg actacgccgg ggatctggag cgcgtcgcgg
    15361 ggcacatcga ccgcacgctg cagctgctca cggaccgcct gcccgagggc gggaccatct
    15421 actggagcgc ggttcccgac gtggcggggc tctatgacat gctgcgcgag cgtgaccaca
    15481 acctgctgtt cgagagctgc caggcgacct gggacctgga cacccggaag gtttccgacg
    15541 gcgcggccca ggacgcctgc gaccacttct tcgacaacga tctgtgccgg tttgcggaca
    15601 cgcaggagca ggccaaggac cgtctcgtgt cgctgttcct cgaccgcctg ctcgcgagcg
    15661 ggggtgtcga ggaaggaccc tgcggcaaga tcctctccag caggtcgacg gatgaagacc
    15721 ggcgcgaggc gcgcgagttc accgcggcgc tgaacggcct gatgcgcgag aaagccgacg
    15781 ccttcgatgg ccgcaacggc gtgcgggtgc gctacagcga gcaggtctac cggccgacgc
    15841 gcccgctcgc gcccgagcac gtctcgcact tcgactgcta ccacccgagt cgcgccgggc
    15901 aggtgttcct cgccgaccag atctggcgcg gcttcgaagg catccgcccg cagggtcacg
    15961 acgtcttcgt ggaggacttc ggcgcgccgc agtactgtcg cgcggacgct gcggcgtgga
    16021 acggctgctg gatcgaagcc aacgacgatg acgaaccggc cagcggcaac gtgcgcatcg
    16081 gcgacggtcg cctgcaggtc ggaggcgcgg gcacgagcct cgaacggggc tttgccctgg
    16141 gtggctactc gcacgcgtgg ctgtcgttca actgggcgcg tgacgatctc gaccgggcgc
    16201 tcgactacgt gagcgtcgag gtatcggccg acggcggcgc gagctgggcc gaggtcgcgc
    16261 gcttcgccgg cgatggcaac gatttcgggc tgcaccgcgg cgactaccac gacctcagcg
    16321 actacgcttc cgcggacacg cgcatccgct ttcgcgcctc gccgggcctg ggctcgggcg
    16381 accgcgtgct gttcgacaac gtgcggctca tcgcgtggaa cgaagtgccc ttcaccgcct
    16441 ggcccgaagt gcccgcgacg ggctcggtgc agagcggggt gggcctgatc cgcggctggg
    16501 cctgcgcggc cgagacggtg tcggtctcga tcgatggcgg cgatcccatc gcggtcgcct
    16561 ggggcggtca gcgtgacgat accgcgggcg tgtgcgggga tgccgccaac ggctacggca
    16621 tggtcctgcc ctgggggctg ctcggcaacg gcccgcaccg gatgcagacc ttcatcgacg
    16681 aggcgcaggt cgggaacgcc gaattcgagg tttccgggct cggcgaggag ttcgtcgagg
    16741 gcctgcaggg cgaatacgtg ctggcgggct tcccggccgc tggcgagcgg gtgcgcctca
    16801 cgtggagcga agcggaccag aatttcctga tcacgggtca cgataccggc gcgggcttca
    16861 ccgatccctc cgcgaccggc atccgggccg atgcagcggc ccgcgcgggc ggggcgcatc
    16921 atgaatcgcc cgcgcagcat tcgatccaga gcggcgtggg cctgatccgg ggctgggcct
    16981 gcgacgcgga ggaagtcacg atctcgatcg acggcggcgc gccgataccg gtcgcccacg
    17041 gcacctcgcg ggccgatacg atcggggtct gcggcgacga ggacaacggc tacggcatgg
    17101 tcttcgcctg gggactgctc ggcaccggcg tgcaccgcct gcagaccttc gtcgacggcg
    17161 tggagatcgc cgacgtcgag ttcgcggtgc aggcgatcgg cgacggtttc gtcgagggac
    17221 tgcagggccg ctacaccctc gacggttttc cggggccggg gcaggcggtg gacgtgcagt
    17281 ggagcgagcc ggaccagaac ttccggatca ttgctgtcca acccgacgct gccggggatt
    17341 gaggttcaga accaatactc ccgcacgcac gtaccattcg ttttcaccga caaggaatcc
    17401 gccatgaata cccgcagtct cctggagcaa ctgctgcaat cgggcagcaa gtacctcggc
    17461 aaccagcccg gcgttgcgaa caaatcgcag ggcggcagcc aggatccgct gtcgggattc
    17521 ctgtccggcg ccgccggcgg cgcgctggcg ggcagcgcga tgagcctgct gctcggcagc
    17581 aagaaggccc gcaaggccac cggcaatctc gcgatgtacg gcggcctcgc ggcactgggc
    17641 gtcgtggcct acaaggcgta cgacaactgg cagaaaacgc aggcgggaat gccgcaatcc
    17701 cagccgcaga ccgtcgatcg cctgagcggc acgcaggccg aggagcacag ccaggcgatc
    17761 ctgcgcgcat tgatcggcgc ggccaaggcc gacgggcaca tcgatgcgcg cgagcgcgcg
    17821 ctgatcgacg gtgaaatcgc caagctcggc gccgaccccg agctggcgcg ctggttcgat
    17881 caggaactcg gcaagccgct ggatcccgcc gaggttgccg ccgcctcgcg ctccactgaa
    17941 atggcagccg agatgtacct cgccagcgtg ctgatcgtcg acgaggacag tttcatggaa
    18001 cgcgcgtacc tgcaggaact tggcaggcag ctgaagctcg c
//
LOCUS       DAORUQ010000049        18007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2766_length_18007_cov_6.699978, whole genome shotgun sequence.
ACCESSION   DAORUQ010000049 DAORUQ010000000
VERSION     DAORUQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18007
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_18007_cov_6.699978"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..880
                     /locus_tag="PLW13_06260"
     CDS             128..880
                     /locus_tag="PLW13_06260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HQY70011.1"
                     /translation="MWISSKGKVAVVTGAGSGIGRAIAVRLARDEAAIAVWDINAEGA
                     AETARMITDAGGKAIAIGADCSDKAAITAAAQRTRAELGAITILVNNAGIAPFTPFMD
                     IGEELFDKVIRINLKGPFLVIREIMPDMVAAKWGRIINITSSSTQSGSVGQTHYVSSK
                     GGLLGMTKALALEYAPTGITVNMVPPGFIDTPMLRASPVDVDAYAASMPMKRVGKPED
                     IAAACAFLASEEAGYITGQTISTNGGRYMGSA"
     gene            complement(862..1689)
                     /locus_tag="PLW13_06265"
     CDS             complement(862..1689)
                     /locus_tag="PLW13_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY70012.1"
                     /translation="MARKSRSRAARVGLSAEADTSDAFEQGHILLADGVEADYPDAMA
                     CLRIAAEQGHAQACNDLATMLRDGLGCAPDIGEAIHWYRLSAAQGEPAAQYNLGLIYL
                     HGIGVEADKALAAEYLLQAANAGDAAAMRELGTMLRLGDGIGKDIVVAAWLHLGAAED
                     GDVAAIEELAAYFRELVPPALGGNVHAARALHRVYRDGLGHEANAALAWAWIRRAHDI
                     CLHDSGGPADEDANAVREDYEQALHAIGAAARKEGDACFEELVRERDSLPPSQAEPM"
     gene            1883..2695
                     /locus_tag="PLW13_06270"
     CDS             1883..2695
                     /locus_tag="PLW13_06270"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY70013.1"
                     /translation="MKFEALALNDRVGSEIRTDVDSLLTPEVAGKVRQLLVERGVLVF
                     RALGLHTEQQLALSRLMGSLREEGQQGLLKITLDKKLNARADYLKGSFLWHIDGTHDD
                     VPPFASLLTGVTLSETGGQTEFANSYAAYEELAQETKDRIAGLRVVHGFETSMRRAGV
                     EPTQDTLAYWRSLPDKAHKLVWTHKSGRKSLVIGCSASHIDGMDREEGQQLLQELLDW
                     STQPRFVYRHEWTPGDLLIWDNTGVLHRAEPYPLDCGRLMHRTTLLGEEPFA"
     gene            complement(2771..3553)
                     /locus_tag="PLW13_06275"
     CDS             complement(2771..3553)
                     /locus_tag="PLW13_06275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70014.1"
                     /translation="MQVAIITGAGSGIGLATARMLHDMGMAIVGIGRDPEKLKALEES
                     IGDPGRLAALSVDITAKDAPKRIVDQALERFGRIDFLVNNAGIGSPKPLHETDDDTLD
                     YFLDIMLRAPFRLAREVIPHMQSGSGVVNVTSTFAMIGGKRGGAYSAAKGGLKSLTEH
                     MACEYGPMGIRSNCVAPGVTMTDMVRHRFEDETFKRVNVETTPYPRLAEAEDIASVIA
                     FLCSAGGEMINGQSIVVDGGWTRTKYLSPRALTSRWISSGEA"
     gene            3730..4626
                     /locus_tag="PLW13_06280"
     CDS             3730..4626
                     /locus_tag="PLW13_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HQY70015.1"
                     /translation="MKALWSTLVVILFSFTMVMEEAHAKRMGGGKSFGRSYQSAPAPA
                     RNAADAPAQRQQADAAKPAQQQGAAKGGLMGGLLGGLLVGGLFAALFAGGAFEGLQLM
                     DILIIAALAFIIFKVIRGLRPGPAAGRSSAPQPAYAGAGRQMPPQPASQPVFGARREA
                     PVAAPVAGSHAVPFNLPPGFDAPAFIAGATEHYRILQDAWNRNDLPTIREYMTPELYA
                     ELSAERASLAVAPQTEVLSLNAELVRADQLFGNAEVSIRFSGRCRDLAEGVEEDIVDV
                     WHLERDFTRGDAPWFITGMQSE"
     gene            complement(4833..6026)
                     /gene="purT"
                     /locus_tag="PLW13_06285"
     CDS             complement(4833..6026)
                     /gene="purT"
                     /locus_tag="PLW13_06285"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HQY70016.1"
                     /translation="MSDSALTLGTPFAAGATRVLFCGAGELGREVVIELKRLGCEVIA
                     VDRYANAPGMQVADRSHVIDMLDPVALRRVILEEKPHLVVPEIEAIATDSLVQLEAEG
                     QRVVPTARAARLTMHREGIRRLAAEELGLATSSYRFADDYASFAAAVREVGFPCVVKP
                     VMSSSGKGQSVLRAEADVQSSWDYAQAGGRAGAGRVIVEGFIDFDYEITLLTVRHAGG
                     TSFCDPIGHLQIDGDYRESWQPQPMSAKALAQAQHIAEGITTALGGWGIFGVELFVKG
                     DEVWFSEVSPRPHDTGLVTLISQQLSEFALHARAILGLPVPAIRQHGPAASCALLVKG
                     DSADMRFGNLGTALAHPDTDLKLFGKPEVHGSRRLGVGLALGASIDEARAKARDVVAA
                     IRVEL"
     gene            complement(6054..8960)
                     /gene="gcvP"
                     /locus_tag="PLW13_06290"
     CDS             complement(6054..8960)
                     /gene="gcvP"
                     /locus_tag="PLW13_06290"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQY70017.1"
                     /translation="MTSQSQPAAAPDLRSLEQNDAFLHRHIGPSEAQIAEMLAVVGAD
                     SLDHLTAQTVPAAILAPAPLNLPEPQPEHVVLERLAGIAAKNRVLRSLIGCGYHDTIT
                     PAVILRNVLENPGWYTAYTPYQPEISQGRLEGLLNFQQMVIDLTGLELANASLLDEGT
                     AAAEAMTLCKRVVRKNRSNTFFVASDCHPQTIAVVRTRAEPLGLEVMVGDPDTDLAGA
                     DVFGALFQYPGTDGRVRELTDLIAQVHAKGALAVVAADLMSLLLLKSPGEMGADVALG
                     NTQRFGVPMGFGGPHAAYFATRDEHKRSMPGRIIGVSIDARGNQALRMAMQTREQHIR
                     REKATSNICTSQALLALMAGFYAIYHGPDRLRTIATRIHRLATILARGLEAGGFSLSH
                     AQFFDTLAVQAGNQQAAIMQRALDAGINLRALDGGRIGLSLDERSTRAEVEALWRIFC
                     ESGAPAFDAIEAASSLSLPAVLLRQDAYLAHPIFNSYHSETEMLRYLRKLESRDIALN
                     RAMIPLGSCTMKLNATTEMIPITWPAFANLHPFAPQDQAAGYAELMQELGDALIECTG
                     YDVLSFQPNSGAQGEYAGLLAIRHYHRSRCEAHRDICLIPSSAHGTNPASAAMAGMRV
                     VIVECDAQGNVDIDDLRLKAERHGEELAALMVTYPSTHGVFEQNIVEICALVHEHGGQ
                     VYLDGANMNALVGIARPGKFGADVSHLNLHKTFCIPHGGGGPGMGPIGVRAHLAPFLP
                     GHVCTPIAGVHDDSMSVSAAPFGSASILPISWVYATLMGAAGLRKATQVAILSANYIA
                     TRLAHCYQVLYTGANGRVAHECIIDLRPIKERSGISEEDIAKRLVDYGFHAPTMSFPV
                     PGTLMVEPTESEPKRELDRFCDAMISIHREVMAVENGQADATDNVLKNAPHTLRDITD
                     AEWQHPYSRSDAAFPVASLHDDKYWAPVNRIDNVYGDRNLFCSCPAVESYREQG"
     gene            9258..10229
                     /locus_tag="PLW13_06295"
     CDS             9258..10229
                     /locus_tag="PLW13_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQY70018.1"
                     /translation="MLLLVTIVSTFGFYRAASGQTDFWGALYMALITISTVGYAEAVP
                     LESAGDRIFAGVISIVGFGALTFMFTSLSMFFLEKDLDHTIRRRRMEKQIAKLRGHYI
                     VCGFGRVGRNTAYELQLTGRRFVAIDLAEERFAEHGERFPGLLWLHGDASDDELLLAA
                     DIGDAAGVFAVTGDDSRNLMITLTARQLNPAVRVVARAHEVRNIAKMRKGGADDVISP
                     DFTGGMRIASAMLRPHAVTFLDEMLRSDRVLRVEEVHIPETFRPTALGAVCRRASEYV
                     LLAVRDGGAWAFNPEEDYVLTPGQVIIAMASTRGREDIEACIADHLG"
     gene            complement(10236..10826)
                     /locus_tag="PLW13_06300"
     CDS             complement(10236..10826)
                     /locus_tag="PLW13_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HQY70019.1"
                     /translation="MNNARDAAYAALRAGAPRPVIAGDAVEAFGARFAAQNGNWARLG
                     SPADIPAWLQARFAPPEGGPLAVVTSADDLLAALPWASAPRLRCAPAHTRAQASLAVT
                     TAMAGVAETGALALHPRAGAPMALNFLPERLVVVVRAADIVATLDDFWTLVRARFGSR
                     LPRGLCLAAGPSTSADVAMTFATGVHGPVELHALVV"
     gene            complement(10823..12256)
                     /locus_tag="PLW13_06305"
     CDS             complement(10823..12256)
                     /locus_tag="PLW13_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HQY70020.1"
                     /translation="MRHSSEQFVANAHAALNDAQLQPVLNAVALFLPAMRTGVLAQTP
                     DFEAMRDYAVRMKDHTLEHLDSYLTDFETRVTERGGHVHHAATAADLNRIVTGICSAA
                     GARRVIKGKSMVGEETALNEALEAAGLEVVETDLGEYIIQQAGEPPSHIIAPALHKSK
                     EQVAQLFRERHARGERDLSQVQGIVREARSVLRERFLAADAGITGANCLVAETGSVML
                     VTNEGNGDLCSTLPRVHIVVTGIEKLVPTLEDAGALHRVLCRSATGQVTSVYTTFYSG
                     PRGAEDPDGPDEFHVVLLDNGRRALLGGKYRSMLRCIRCGACLNHCPIYRHVGGHAYG
                     WVYPGPMGSVLTPLLTGLDQARELPNACTACGRCEEACPMRIPLPEHLRQLRDDAAAQ
                     GLTPPAWRAGVSAMMTLFRFPALYRVAAALGRPLLGWLSRHPALLAKLPLLSGWGAGR
                     RLPVPQGHTFLGHWQKVHGARDPEQRL"
     gene            complement(12256..13029)
                     /locus_tag="PLW13_06310"
     CDS             complement(12256..13029)
                     /locus_tag="PLW13_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQY70021.1"
                     /translation="MPAPSPPRIALFVTCLANVFRPAVAEASVELLRAAGCEVHVPLA
                     QSCCGQPGYNSGAIASARPVAQGLIRTFEDFDYVVAPSGSCAGMIRHHYPRLFADDPR
                     WRERAQALGAKTFELTAFLAEVMHFAPAPTRGTAARTITYHDSCAGLRELGIRAQPRQ
                     LLAAAGVTLTEMQGTEVCCGFGGTFCAKLPEISVAMADEKLGNALAAGADMLVGGDLG
                     CLLHLCGRAQAAGKSLEFRHVSELLAARLDRAALGEEQP"
     gene            complement(13116..13622)
                     /gene="greB"
                     /locus_tag="PLW13_06315"
     CDS             complement(13116..13622)
                     /gene="greB"
                     /locus_tag="PLW13_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HQY70022.1"
                     /translation="MLRPRPGGPRKSPYITPQGEQRLREELTQLWKVERPQVTAAVHE
                     AAKNGDRSENGDYIYGKRRLREIDRRVRYLTKRLDEVKVVREVHADRGKVYFGAWITL
                     EDEDGKALRYRIVGPDEFDVAKGLISMDSPVARAVLGKRLDDEIRVHTPAGEREYVVT
                     AIEYPYDN"
     gene            13719..14558
                     /locus_tag="PLW13_06320"
     CDS             13719..14558
                     /locus_tag="PLW13_06320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY70023.1"
                     /translation="MSAVLTRILSALLVALAAPWACALELDGPLVQGGVVIGKVAPGT
                     RVALDGRAVRVAADGRFVIGFDRDAPVKHVLKAGAQTRELEIARRDYDIQRVTGIAQA
                     IMSPSAEDLKRIQREQSLVVAARARDDARTDFAAGFVWPITGRISGVYGSQRFYNGEP
                     SRPHYGVDVAAPVGAEVRAPAPGVVTLAQPDLFYSGGTVIIDHGHALSSSFLHLSKVL
                     VEPGQRVKQGELIARVGATGRATGPHLDWRMNWRDAKVDPQLLAGAMPASPQAPVAQG
                     DMN"
     gene            14561..14746
                     /locus_tag="PLW13_06325"
     CDS             14561..14746
                     /locus_tag="PLW13_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQY70024.1"
                     /translation="MDPKLLEILVCPVSKGPLEYDREKQELVCRASGLAYPVRDGIPV
                     MLESEARQLTLEERERG"
     gene            14867..17467
                     /gene="alaS"
                     /locus_tag="PLW13_06330"
     CDS             14867..17467
                     /gene="alaS"
                     /locus_tag="PLW13_06330"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQY70025.1"
                     /translation="MNGAEIREAFLSYFESMGHTRVPSSSLVPGNDPTLLFTNAGMVQ
                     FKDCFLGREQRAYVRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     HDAIRFAWEFLTGVLALPREKLWVTVFQDDLEAEKIWIEEIGVPAERCVRLGEKSNFW
                     SMGDTGPCGPCTEIFYDHGPGVAGGPPGSPDEDGDRYIEIWNLVFMQFERSADGSMTP
                     LPKPSVDTGMGLERIAAVMQHVHSNYEIDLFRRLLDAAQQVTGATDPASPSLRVIADH
                     IRSCAFLVSDGVLPSNEGRGYVLRRILRRACRHGHKLGAQDTFFHKLVGPLAEAMGDA
                     YPELHEKRAQIEKVLLLEEEQFARTLDTGMRILEQDIAALAGDTLDGDTVFKLYDTFG
                     FPMDLTADIARERGLLVDEEGFERAMQAQRERARGASHFAAEQYGDAGIDTPTVFTGY
                     EALAGEARVLALLRDGQRVEGLAEGEKGIVVLDRTPFYAESGGQAGDAGVIEAGGWRF
                     EVRDTTKAGAISLHHGVMARGTLAVGAAADARVEPGIRQATALNHSATHLLHAALRQV
                     LGEHVAQKGSLVDAERLRFDFSHFEAMTPVQIRAVERLVNEQIRANSAVEIQLTDMDT
                     AKAKGAMALFGEKYGDEVRVLTMGGGFSVELCGGTHVQRTGDIGLMRVTAESGIAAGV
                     RRIEAVTGANALALFDTLEARGDRIAQLLKSGRDNAVEKVEQLIAANRQLEKELEKLR
                     VQLASGTGRDLAAGAIDVNGVKVLAAQLEGADAKSLRTTMDQLKDKLGTAVILLAAVE
                     GDKVSLVAGVTGGLQERVRAGDLMAEIAPLLGGKGGGRADMAQGGGTDAAALPQALQR
                     VPEWVRARLA"
     gene            17647..17841
                     /gene="csrA"
                     /locus_tag="PLW13_06335"
     CDS             17647..17841
                     /gene="csrA"
                     /locus_tag="PLW13_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HQY70026.1"
                     /translation="MLILTRRVGETLMIGDSVTITVLGVKGNQVRLGVNAPKDVSVHR
                     EEIYQRIQREHDGEDSEPSS"
     gene            17912..18002
                     /locus_tag="PLW13_06340"
     tRNA            17912..18002
                     /locus_tag="PLW13_06340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17946..17948,aa:Ser,seq:gct)
BASE COUNT         2804 a         5971 c         6365 g         2867 t
ORIGIN      
        1 gtcgatccct acccggtgga ggaaggtcgc ctgatgcacc gcaccacgat cgaggcggag
       61 gaagcactgg cctgatcgag cggcttcaag gcgaaacaaa accgctccca aggcgaggat
      121 tcggagtatg tggatttcga gcaagggcaa ggttgccgtc gtcaccggtg ccggttcggg
      181 tatcggccgg gccatcgcgg tacggctggc gcgcgatgaa gcggccatcg cggtctggga
      241 catcaatgcc gaaggcgccg ccgaaacggc caggatgatc acggacgccg gcggcaaggc
      301 cattgccatc ggcgccgatt gctccgacaa ggcggcgatc acggcggcgg cgcagcggac
      361 ccgcgccgag ctcggcgcga tcaccatcct cgtcaacaat gcgggcattg ctccgttcac
      421 gccgttcatg gacatcggcg aggagctctt cgacaaggtg atccgcatca acctcaaggg
      481 cccgttcctc gtcatccggg agatcatgcc cgacatggtg gcggcgaagt ggggccgcat
      541 catcaatatc acctcgtcct cgacccagtc gggctcggtc ggccagacgc actacgtgtc
      601 gtccaagggc ggtctgctcg gcatgacgaa ggcgctggca ctggaatacg cacccaccgg
      661 catcaccgtg aacatggtgc caccgggctt catcgatacg ccgatgctgc gcgcctcccc
      721 ggtcgacgtc gatgcctacg cggcctccat gccgatgaag cgcgtcggca agcccgagga
      781 cattgccgcg gcctgcgcct tccttgcgtc ggaggaagcc ggctacatca ccggccagac
      841 gatcagcacg aacggtggcc gttacatggg ttcggcctga gagggtggca gggagtcgcg
      901 ctccctgacg agttcctcga agcaggcgtc cccctccttg cgggcggcgg ctccgatcgc
      961 gtgcagcgcc tgctcgtagt cttcccgcac ggcgttcgcg tcttcatccg ccggtcctcc
     1021 ggaatcgtgc aggcagatat cgtgcgcccg gcgtatccac gcccaggcca gcgctgcgtt
     1081 ggcctcatgt ccgagtccgt cgcggtagac gcgatgcaag gccctggcgg catgcacatt
     1141 gccgcccagg gcgggaggga cgagttcgcg gaagtaagcg gcgagctctt cgatggcagc
     1201 gacatcgccg tcctccgcgg cgccgaggtg cagccatgca gcgacgacga tgtccttgcc
     1261 gatgccgtcg cccagcctca gcatggtccc cagctcgcgc atcgcggccg cgtcaccggc
     1321 attggctgcc tgcagcaggt actccgccgc cagggccttg tcggcttcga cgccgatgcc
     1381 gtgcaggtaa atgaggccga ggttgtactg cgcggcgggc tcgccctgcg cggcgctcag
     1441 ccgataccag tggatcgcct ccccgatgtc gggagcgcac cccaggccgt cgcgcagcat
     1501 cgttgccagg tcgttgcatg cctgcgcatg tccctgctcc gcggcgatgc gcaaacaggc
     1561 cattgcgtcg ggatagtcag cctcgacgcc atcggcgagc aggatgtgcc cctgctcgaa
     1621 tgcgtcgctg gtatccgctt cggcggacaa tcccaccctg gcggcgcgtg atctggattt
     1681 tctggccatg atttgccttg cggtggcggt gcgggcgcat cgtatcaggc tcgcgccgcc
     1741 atggcctcga tcggcaccgg gacgatgagc ggcgccagag cgagacgccg cggcgcgcgg
     1801 cgaggtcgca ccgggtggtg gacggatttg acgcaaaagt actgagcggt atcataaagc
     1861 gcaaaccagg gagagaacgg ccatgaagtt tgaagcgctc gcgctcaatg atcgtgttgg
     1921 atccgagatt cgcaccgatg tcgacagcct gttgaccccg gaggtcgcgg ggaaagtccg
     1981 tcagctgctg gtcgagcgcg gcgtgctggt attcagggcg ctcgggctgc acaccgagca
     2041 acagctcgcc ctgtcccgac tgatgggttc cctgcgcgag gaaggtcaac agggcctgct
     2101 gaagatcacg ctcgacaaga aactcaacgc aagggcggat tacctgaagg gatcgttcct
     2161 gtggcacatc gacggcacac acgatgatgt gccgcccttc gcttcgctgc tgacgggtgt
     2221 cacgctgtcc gaaaccggcg gccagaccga attcgccaac tcctatgccg cttacgaaga
     2281 gttggcgcag gagacgaagg atcgcatcgc cgggctcagg gtggtacacg gcttcgagac
     2341 gtctatgcgc agggcgggcg tcgagcccac gcaggacacc ctcgcctatt ggcgcagctt
     2401 gcccgacaag gcccacaagc tggtctggac gcacaaatcg ggtcgcaagt cgctggtcat
     2461 cggctgttct gcttcccata tcgacggcat ggacagggaa gaggggcagc aactgctgca
     2521 ggaactgctg gactggagca cccagccgag gttcgtctat cgccacgagt ggacgccggg
     2581 agacctgctg atctgggaca acaccggcgt gctgcaccgc gcggagccct atccgctgga
     2641 ttgtggccgc ttgatgcacc gcaccacgct gttgggcgag gagccgttcg cctgagcgag
     2701 cagggagcct ggcggtccag gcggtcgcgg gggatgcccg cgacgtgagg gcgctgtctt
     2761 tgcgctaccg tcaggcttcg ccggaggaaa tccatcgcga cgtcagcgcg cgcggcgaaa
     2821 ggtacttggt cctggtccag cccccgtcaa cgacgatcga ctgcccgttt atcatttcgc
     2881 cccccgccga acacaggaag gcgatgacgc tggcgatgtc ctcggcctcg gcaaggcgcg
     2941 gatagggcgt ggtttcgaca ttgacacgct tgaacgtctc gtcctcgaag cgatggcgca
     3001 ccatgtccgt catggtgacg cccggcgcca cgcaattgga gcggatgccc atcgggccat
     3061 actcgcaggc catgtgctcg gtgagcgatt tcagcccgcc cttcgccgcc gaataggcgc
     3121 cgccgcgctt gccaccgatc atcgcgaagg tggacgtcac gttgaccacg cccgaaccgg
     3181 attgcatgtg ggggatcacc tcgcgcgcca ggcgaaaggg cgcgcgcagc atgatgtcga
     3241 ggaaatagtc gagcgtgtcg tcgtcggtct cgtgcagcgg cttggggctg ccgatgccgg
     3301 cgttgttgac gaggaagtcg atgcgcccga accgttccag cgcctggtcg acgatacgct
     3361 tcggcgcgtc tttcgcggtg atatcgacag acaaggccgc gagccgcccg gggtcgccga
     3421 tcgattcttc cagtgctttg agtttttccg ggtcgcgccc gatgccaacg atcgccatgc
     3481 ccatgtcgtg gagcatcctg gctgttgcga ggccgatgcc gcttcccgcg cccgtgatga
     3541 ttgctacctg catgactgtt ttcccgtctg gtccgtgatg gctcatgctg gggcaagcgc
     3601 aaacgtcaat caaggtccgg atccgcgtgc cggagaagct gcagtcttga cttgtggctg
     3661 cgatgccgca gactcaccgc cggcattgca ccggccgccg ggccatcgat tcgtcaaccg
     3721 aggtaattca tgaaggcgtt gtggtcgaca ctcgttgtca ttctgttcag cttcaccatg
     3781 gtcatggaag aggcccacgc caagcgcatg ggcggcggca agtctttcgg tcgcagctac
     3841 cagagcgcgc cggccccggc caggaacgcg gccgacgccc cggcccagcg tcaacaggcc
     3901 gatgcggcca agccggccca gcagcagggt gccgcgaagg gtgggctgat gggtggtctg
     3961 cttggcgggc tgctggtggg cggcctgttt gcggccctgt tcgccggtgg cgctttcgaa
     4021 gggctgcagt tgatggacat cctgatcatc gccgccctgg cgttcatcat cttcaaggtg
     4081 atccgcggcc tgcgcccggg ccccgccgcg ggccgctcat cggcgccaca gccggcttac
     4141 gccggcgcgg gccggcagat gccgccgcag cctgcgtccc agccggtatt cggtgcccgt
     4201 cgcgaggcgc cggtcgccgc gccggtggca ggcagccacg ccgtgccctt caacctgccg
     4261 ccgggctttg atgccccggc cttcatcgcg ggcgccacgg agcactaccg tatcctgcag
     4321 gatgcctgga accgtaacga cctgcctacg atccgggagt acatgacccc cgagctgtac
     4381 gccgagttga gcgccgagcg tgccagcctt gccgtcgcgc cgcagaccga ggtgctcagc
     4441 ctgaacgcgg agctggtacg tgccgatcag ctgttcggca atgccgaggt cagcatccga
     4501 ttcagcggcc gttgccgcga tctcgccgaa ggcgtggaag aggatattgt cgacgtgtgg
     4561 cacctggagc gtgacttcac caggggcgat gcgccctggt tcatcacggg catgcagtcg
     4621 gagtaatcca tttccgccat tgcgccgtgc agccgcctgc gctgaatggt gttgcggccg
     4681 ggcttgcccg ggcctggacg cccgcgcggg cgtccagcac attggccagc ttgccgctgc
     4741 aataggcgag gccgctggag taattgccca ggttctgcag gtcctcctgc tcccgctgac
     4801 gcgctcgcgc gtcacgcggg acgttgacag ttctacagct cgacccggat ggcggcgacg
     4861 acgtcgcgcg ccttggcgcg tgcctcgtcg atgctggccc cgagtgccag cccgacgccg
     4921 agacggcgcg aaccatgcac ctcgggcttg ccgaagagct tgaggtcggt gtcgggatgc
     4981 gccagcgccg tacccaggtt gccgaagcgc atatcggcgg aatcgccctt gaccagcagg
     5041 gcgcaggagg cggccggccc gtgctggcgg atggcgggga cgggcaggcc gaggatggcg
     5101 cgggcatgca gcgcgaactc ggaaagttgc tgtgagatca gcgtcacgag gccggtgtcg
     5161 tgcggacgcg gcgatacctc gctgaaccag acctcatcgc ccttcacgaa gagctccacg
     5221 ccgaagatgc cccagccgcc aagcgcggtg gtgatccctt ccgcgatgtg ctgtgcttgc
     5281 gccagcgcct tggccgacat gggctgtggc tgccaggact cgcggtagtc gccgtcgatc
     5341 tgcaggtggc cgatgggatc gcagaagctg gtgccaccgg catgacgcac ggtgagcagc
     5401 gtgatctcgt agtcgaaatc gatgaagccc tcgacgatga cgcgccccgc gccggcacga
     5461 ccgcccgcct gggcatagtc ccaggacgac tgcacgtcgg cctcggcgcg cagcacgctc
     5521 tggcccttgc cggacgagga catgaccggc ttcaccacgc agggaaagcc gacctcgcgc
     5581 acggcggccg cgaagctggc gtagtcgtcg gcgaagcgat aggaggaggt ggccaggccc
     5641 agttcctcgg ccgccaggcg acggatgccc tcacggtgca tggtcaggcg cgcggcacgc
     5701 gccgtgggca ccacgcgctg gccctcggcc tcgagctgca ccagggagtc ggtggcaatg
     5761 gcctcgatct ccggcaccac caggtggggc ttttcctcga ggatgacgcg acgcagcgcc
     5821 accggatcca gcatgtcgat gacgtggctg cggtcggcaa cctgcatgcc gggggcgttg
     5881 gcgtagcggt ccaccgcgat cacctcgcag ccgagtcgct tgagctcgat gacgacctcg
     5941 cggccgagct cgccggcacc gcagaacagc acgcgggtgg cgccagcggc gaagggggta
     6001 cccagggtca gggcggagtc ggacattgac ggtttccttt gcgtttccac cactcagccc
     6061 tgttcgcggt agctttccac cgcggggcac gagcagaaca ggttgcgatc gccgtagacg
     6121 ttgtcgatgc ggttgaccgg cgcccagtac ttgtcgtcgt gcaggcttgc gacggggaag
     6181 gccgcgtcgc tgcgcgagta ggggtgctgc cactcggcgt cggtgatgtc gcgcagcgtg
     6241 tgcggggcgt tcttcagcac gttgtcggtc gcgtcggcct ggccattctc caccgccatc
     6301 acttcgcggt ggatgctgat catcgcgtcg cagaagcggt cgagctcgcg cttgggctcg
     6361 ctctcggtgg gctccaccat cagcgtgccg ggcaccggga atgacatcgt gggtgcgtgg
     6421 aagccgtagt cgaccaggcg cttggcgatg tcttcctcgc tgatgccgct gcgctccttg
     6481 atcgggcgca ggtcgatgat gcactcgtgt gccacgcggc cattggcacc ggtgtacagc
     6541 acctggtagc agtgcgccag gcgcgtggcg atgtagttcg cgctgaggat cgcgacctgc
     6601 gtggccttgc gcagccccgc ggctcccatc agcgtggcgt agacccagga gatcggcagg
     6661 atgctcgcgc tgccaaaggg ggctgccgac accgacatcg agtcgtcgtg cacgccggcg
     6721 atcggcgtgc agacgtgacc cggcaggaag ggcgcgaggt gtgcgcgcac cccgatcggg
     6781 cccatgccgg ggccgccgcc gccgtgcggg atgcagaagg tcttgtgcag gttcaggtgc
     6841 gagacgtcgg cgccgaactt gccagggcgc gcgatgccga ccagcgcgtt catgttcgcg
     6901 ccgtccaggt acacctggcc gccgtgctcg tggaccagcg cgcagatctc gacgatgttc
     6961 tgctcgaaca ccccgtgcgt ggacgggtag gtgaccatca gcgcggcgag ctcctcgccg
     7021 tgtcgctcgg ccttcaggcg caggtcgtcg atatcgacgt tgccctgcgc gtcgcactcc
     7081 acgatcacca cgcgcatgcc ggccatcgcg gcgctggcgg ggttggtgcc gtgcgcggaa
     7141 ctggggatca ggcagatgtc gcggtgcgcc tcgcagcgcg agcggtggta gtggcggatc
     7201 gcgagcagtc ccgcgtactc gccctgcgcg cccgagttcg gctggaacga cagcacgtcg
     7261 tagccggtgc actcgatcag cgcatcgccc agttcctgca tcagttccgc gtagcccgcg
     7321 gcctggtcct gcggcgcgaa ggggtgcagg ttggcgaagg ccggccaggt gatcgggatc
     7381 atctcggtgg tggcgttcag cttcatggtg caggagccga gcgggatcat cgcgcggttc
     7441 agcgcgatgt cgcggctctc gagcttgcgc aggtagcgca gcatctcggt ctcgctgtgg
     7501 tagctgttga agatcgggtg cgcgaggtag gcgtcctggc gcagcagcac cgcgggcagc
     7561 gacagcgagg aggcggcttc gatcgcgtcg aaggcgggcg cgccggactc gcagaagatg
     7621 cgccacagcg cttcgacctc ggcgcgcgtg ctgcgctcgt ccaggctcag gccgatgcgc
     7681 ccgccgtcga gcgcgcgcag gttgatgccg gcatccagcg cgcgctgcat gatcgctgcc
     7741 tgctggttgc cggcttgcac ggcgagcgta tcgaagaatt gcgcgtgcga caggctgaaa
     7801 ccgccggcct cgagaccgcg cgcgagtatc gtggcgaggc ggtggatgcg cgtcgcgatc
     7861 gtgcgcagcc gatcgggacc gtggtagatc gcgtagaacc ccgccatcag ggccagcagc
     7921 gcctgcgagg tacagatgtt gctggtcgcc ttctcgcggc ggatgtgctg ctcgcgcgtc
     7981 tgcatcgcca tgcgcagcgc ctggttgccg cgcgcatcga tcgagacgcc gatgatgcgt
     8041 ccgggcatcg agcgcttgtg ctcgtcgcgc gtggcgaagt aggccgcgtg cgggccgccg
     8101 aatcccatcg gcacgccgaa gcgctgcgtg ttgccgagcg ccacgtcggc gcccatctcg
     8161 cccgggctct tcagcagcag caggctcatc aggtcggccg cgaccacggc cagcgcgccc
     8221 ttggcgtgca cctgcgcgat caggtccgtg agctcgcgca cgcggccatc ggtgcccggg
     8281 tactggaaca gggcgccgaa gacgtcggcg cccgcgagat cggtgtcggg gtcgcccacc
     8341 atgacctcga ggccgagcgg ctcggcgcgc gtgcgcacca cggcgatggt ctgcgggtgg
     8401 cagtccgacg ccacgaagaa ggtgttcgac cggttcttgc gcaccacgcg cttgcacagc
     8461 gtcatggcct cggccgccgc ggtgccctcg tcgagcagcg aggcgttggc gagttcgagc
     8521 cccgtgaggt cgatgaccat ctgctggaag ttcagcagcc cttccagccg gccctgcgat
     8581 atctcgggct ggtagggcgt ataggcggtg taccagcccg ggttttccag cacgttgcgc
     8641 aggatcaccg ccggcgtgat ggtgtcgtgg tagccgcagc cgatcagcga gcgcagcacg
     8701 cggttcttcg ccgcgatgcc tgccaggcgt tccagcacaa cgtgctcggg ctgcggctcg
     8761 ggcaggttaa gcggcgcggg ggcgaggatc gccgccggca cggtctgcgc ggtcaggtgg
     8821 tcgagcgagt cggcgcccac gacggccagc atctcggcga tctgcgcctc ggacgggccg
     8881 atgtggcggt gcaggaaggc atcattctgc tcgagggagc gaagatcggg ggctgcggcg
     8941 ggctgcgact ggctggtcat ggctttactc ggaaaggccg tgcgggggcg gcggagcggg
     9001 ttccggaaag cgccgtattt aaccacagcg tccgggccct gccgaatgcg gatttcggga
     9061 aaaagagccg gtccgtcgcc gcttgagccg cggcccgcat tgtccgtaac atggcgcgca
     9121 acagccctgt cattccaggt cccgctcctc aagtgcccca attgcgcagg aaaccccgcg
     9181 tcccgcggca tcgaccgccg ggtctcaaga attcggcgct gccacggctc acgctgcgga
     9241 tcatggcggc ggccgtcgtg ctccttctgg tgaccatcgt cagcactttc ggcttctacc
     9301 gcgcggccag cggccagacc gacttctggg gcgcgctgta catggcgctg atcacgatct
     9361 ccacggtggg ctacgccgag gcagtgccgc tcgagagcgc gggcgaccgc atattcgccg
     9421 gcgtgatctc gatcgtggga ttcggtgcgt tgaccttcat gttcaccagc ctctcgatgt
     9481 tcttcctcga gaaggatctc gaccacacga tccggagacg ccgcatggag aagcagatcg
     9541 ccaagctcag gggccactac atcgtgtgcg gtttcggccg tgtcgggcgc aataccgcct
     9601 atgagctgca gctgaccggg cggcgcttcg tcgcgatcga cctggccgag gagcgattcg
     9661 cggagcatgg cgaacggttc ccggggctgc tgtggctgca cggcgacgcc tccgatgacg
     9721 aactgctgct ggcggcggat atcggcgatg ccgccggcgt gttcgcggtg accggtgatg
     9781 actcgcgcaa cctcatgatc acgctcaccg ccaggcagct gaacccggcc gtgcgcgtgg
     9841 tggcgcgcgc ccacgaggtg cgcaatatcg cgaagatgcg caagggcggg gcggacgacg
     9901 tgatctcgcc cgatttcacc ggcggcatgc gcatcgcctc ggcaatgctg cgcccgcatg
     9961 cggtgacgtt cctcgatgaa atgctgcgct ccgatcgcgt cctgcgcgtg gaagaggtgc
    10021 acataccgga gacgttccgc ccgaccgcgc tcggcgcggt gtgccgccgc gccagcgaat
    10081 acgtgctgct cgcggtgcgc gacggaggcg cgtgggcctt caatccggaa gaggactacg
    10141 tgctcacgcc ggggcaggtg atcatcgcga tggcgagcac gcgcggtcgg gaagacatcg
    10201 aggcctgcat cgcggaccac ctgggctgag aggcctcaga caaccagcgc gtgcaactcc
    10261 acggggccgt gcacgccggt cgcgaacgtc atggccacgt cggccgatgt cgagggcccg
    10321 gccgcgaggc acagtccgcg cggcaggcgg ctgccgaagc gtgcgcgcac cagggtccag
    10381 aagtcgtcca gcgtggccac gatgtcggcc gcgcgcacca cgaccaccag ccgctcgggc
    10441 aggaagttca gcgccatcgg cgcgccggcg cgcggatgca gggccagcgc gcccgtctcg
    10501 gcaacgccgg ccatggctgt ggtcacggcc agcgaggcct gcgctcgcgt gtgcgcgggc
    10561 gcgcaccgca ggcgcggtgc gctggcccat ggcagcgcgg ccagcagatc gtccgcggat
    10621 gtcaccacgg ccagcgggcc gccttccggc ggcgcaaagc gcgcctgcag ccacgcggga
    10681 atgtccgcgg gcgagcccag tcgcgcccag ttgccgttct gggcggcgaa acgcgcgccg
    10741 aaggcctcga ccgcgtcgcc cgctatcacg gggcgcggcg cacccgcgcg caacgccgcg
    10801 taagccgcat cgcgcgcgtt gttcacagtc gttgctccgg gtcgcgggcg ccgtgcactt
    10861 tctgccagtg cccgaggaag gtgtgcccct gcggcacggg caggcgccgc cccgcgcccc
    10921 agccggaaag cagcggcagc ttcgcgagca gcgcggggtg gcgcgacagc cagccgagca
    10981 gcgggcgacc cagcgccgca gccacgcggt aaagcgccgg gaaacggaac agcgtcatca
    11041 tcgcgctcac gcccgcgcgc cacgccggcg gcgtgaggcc ctgcgccgcg gcatcgtcgc
    11101 gcagttggcg caggtgctcg ggcagcggga tgcgcatcgg gcaggcttcc tcgcagcgcc
    11161 cgcaggcggt gcaggcgttg ggcagttcgc gcgcctgatc gagacccgtc agcagtggcg
    11221 tcagcaccga gcccatcggg ccgggataga cccaaccata ggcatggccg ccgacgtggc
    11281 ggtagatcgg gcagtggttc aggcaggcgc cgcagcggat gcagcgcagc atgctgcggt
    11341 acttgccccc gagcagcgcg cggcgaccgt tgtcgagcag cacgacgtgg aattcatcgg
    11401 gtccgtcggg gtcttcggcg ccgcgcggcc cgctgtagaa ggtggtgtag acgctggtga
    11461 cctgcccggt cgcgctgcgg cacagcacgc ggtgcagcgc gccggcatcc tcgagcgtgg
    11521 gcaccagctt ctcgattccg gtcaccacga tgtgcacgcg cggcagcgtg ctgcacagat
    11581 cgccgttgcc ctcgttggtg accagcatca ccgagccggt ctccgcgacc aggcagttgg
    11641 cgccggtgat gccggcgtcg gccgccagga aacgctcgcg cagcacgctg cgcgcctcgc
    11701 gcacgatgcc ctgcacctgc gacaggtcgc gctcgccgcg cgcgtggcgc tcgcggaaca
    11761 gttgcgcgac ctgctccttc gacttgtgca gcgccggggc gatgatgtgg ctcggcggct
    11821 cgccggcctg ctggatgatg tactcgccga ggtcggtctc gaccacctcg agcccggcgg
    11881 cctcgagcgc ttcgttcagc gcggtctcct cgccgaccat cgacttgccc ttgatcacgc
    11941 gccgcgcgcc cgcggcgctg cagatgccgg tgacgatgcg gttcaggtcg gcggcggtgg
    12001 cggcgtggtg cacatggccg ccgcgttccg tgacgcgggt ctcgaagtcg gtgaggtagc
    12061 tgtcgaggtg ctccagcgtg tggtccttca tgcgcaccgc gtagtcgcgc atcgcctcga
    12121 agtcgggggt ctgggccagc acgccggtgc gcatcgccgg cagaaacagg gccacggcgt
    12181 tcagtaccgg ttgcagctgt gcgtcgttca gcgctgcgtg cgcgttggcg acgaactgtt
    12241 cgctgctgtg gcgcatcagg gctgctcctc gccgagcgcg gcgcggtcca gccgtgcggc
    12301 cagcagctcg ctcacgtggc gaaattccag ggacttgccg gcagcctgcg cgcggccgca
    12361 caggtgcagc aggcaaccga ggtcgccgcc gaccagcatg tcggcgcccg cggccagtgc
    12421 attgccgagc ttctcgtcgg ccatcgcgac ggagatttcc gggagcttgg cgcagaaggt
    12481 gccgccgaag ccgcagcaga cctcggtgcc ctgcatttcg gtcagtgtca cgcccgccgc
    12541 ggcgagcagc tgccgcggct gcgcccggat gccgagctcg cgcaggccgg cgcagctgtc
    12601 gtggtaggtg atcgtgcgcg ccgcggtgcc acgcgtcggc gcgggagcga aatgcatcac
    12661 ctcggcgagg aaggccgtga gctcgaaggt ctttgcgccg agcgcctgcg cgcgctcgcg
    12721 ccaccgcgga tcatcggcga acagccgcgg gtaatggtgg cggatcatgc cggcgcagga
    12781 gccagacggc gcgaccacgt agtcgaagtc ctcgaacgtg cggatgaggc cttgcgccac
    12841 cgggcgcgca ctggcgatcg cgccgctgtt gtagccgggc tggccgcagc aggattgcgc
    12901 gagcggcaca tggacctcgc agcccgcggc gcgcagcagt tccacgctgg cctcggccac
    12961 ggccggacgg aaaacgttgg cgaggcaggt cacgaacagc gcgatgcgcg gaggcgaggg
    13021 agccggcatt ggggtgtgcc tcggttcgat gggaggcagt atatccggca attgcacgtg
    13081 ggtccgcatt cacgcggaca ggagcggttg caggatcaat tgtcgtacgg gtattcgatc
    13141 gcggtgacga cgtactcgcg ttcgcccgcc ggcgtatgca cgcggatctc gtcgtcgagg
    13201 cgtttgccga ggacggcgcg cgcgaccggg gaatccatgc tgataagccc cttcgccacg
    13261 tcgaactcgt cgggcccgac gatgcggtag cgcagcgcct tgccgtcctc gtcctccagc
    13321 gtgatccagg cgccgaagta caccttgccg cggtccgcgt gcacctcccg cacgaccttc
    13381 acctcgtcga ggcgcttggt gaggtagcgc acgcggcggt cgatctcgcg caggcggcgc
    13441 ttgccgtaga tgtagtcgcc attctccgag cggtcaccgt tcttggcggc ctcgtgcacc
    13501 gccgccgtga cctgcgggcg ctcgaccttc cacagctgcg tcagctcctc gcgcaggcgc
    13561 tgctcgccct gcggggtgat atagggcgac ttgcgcgggc cgccggggcg agggcggagc
    13621 atgcgaatcc tccggggggt tgcggcgccg cgccggcgct atctggtaga ctgcactcta
    13681 tccaccaaac tgccgcattg accagaactc cagccccgat gtccgccgta ctcacacgaa
    13741 tactgtccgc gctgctcgtg gcgctggccg cgccgtgggc ctgcgccctc gaactcgacg
    13801 gcccgctggt gcagggcggg gtggtgatcg gcaaggttgc ccccggcacg cgggtcgcgc
    13861 tcgacggacg ggcggtgcgc gtggccgccg acggccgctt cgtgatcggt ttcgaccgcg
    13921 atgcccccgt gaagcacgtg cttaaggccg gtgcgcagac acgcgaactg gagatcgccc
    13981 ggcgcgacta cgacatccag cgcgtcaccg gcatcgcgca ggcgatcatg agcccctcgg
    14041 ccgaggacct caaacgcatc cagcgcgagc agtcgctcgt ggtcgcggcg cgcgcgcgcg
    14101 atgacgcgcg caccgatttc gcggccggtt tcgtgtggcc gatcaccggg cgcatcagcg
    14161 gcgtctacgg cagccagcgc ttctacaacg gcgagccctc gcgcccgcac tacggggtgg
    14221 acgtggctgc tcccgtgggt gccgaggtgc gcgcgccggc tcccggcgtc gtgacgctgg
    14281 cccagccgga cctgttctac tcgggcggta ccgtgatcat cgaccacggt cacgcgctgt
    14341 cctcgagctt cctgcatctg agcaaggtgc tggtcgagcc gggacagcgg gtgaagcagg
    14401 gcgagctgat tgcccgcgtg ggcgccaccg ggcgcgccac cgggccgcac ctcgactggc
    14461 gcatgaactg gcgcgacgcc aaggtcgatc cccaattgct ggcgggcgcg atgcccgcct
    14521 caccccaggc gcccgtcgca caaggagaca tgaactgatg ctggatccga aactgctgga
    14581 aatcctcgtc tgccccgtca gcaaggggcc gctggaatac gaccgcgaga agcaggagct
    14641 ggtctgccgc gccagcggcc tggcctaccc ggtgcgcgac ggcatcccgg tgatgctcga
    14701 gagcgaggcg cgccagctca cgctcgagga acgcgagcgc ggctgagcgc gacgtgattc
    14761 ttcacgcggg acgccggctc cgcgcgtccg tataattccc cgcctttacc gcccccggcc
    14821 gcccggccgc gggcggccac ccgacgccac acgcggaagc agatcaatga atggtgccga
    14881 gatcagggaa gctttcctga gctatttcga gagcatgggt cacacgcgcg tgcccagcag
    14941 ttcgctggtg ccgggcaacg acccgacgct gctgttcacc aacgccggca tggtgcagtt
    15001 caaggactgc ttcctcggtc gcgagcagcg cgcctacgtg cgcgcggcga gctcgcagcg
    15061 ttgcgtgcgc gccggcggca agcacaacga cctcgagaac gtgggctaca ccgcgcgcca
    15121 ccacaccttc ttcgagatgc tcggcaactt cagcttcggc gactacttca agcacgacgc
    15181 gatccgtttc gcctgggaat tcctgaccgg ggtgctcgcg ctgccgcgcg agaagctctg
    15241 ggtcacggtg ttccaggacg acctcgaggc cgagaagatc tggatcgagg agatcggcgt
    15301 gccggccgag cgctgcgtgc gtcttggcga gaagtcgaac ttctggtcca tgggcgacac
    15361 cggcccctgc ggcccctgca ccgagatctt ctacgaccac ggccccgggg tcgcgggcgg
    15421 gccgccgggc agccccgacg aggacggtga ccgctacatc gagatctgga acctggtgtt
    15481 catgcagttc gagcgctccg ccgacggcag catgacgccg ttgcccaagc cctcggtcga
    15541 caccggcatg gggctcgagc gcatcgcggc cgtgatgcag cacgtgcact cgaactacga
    15601 gatagacctg ttccgccgcc tgctcgatgc cgcgcagcaa gtcaccggtg ccaccgatcc
    15661 cgccagtccc tcgctgcgcg tgatcgccga ccacatccgc tcctgtgcct tcctggtgag
    15721 cgacggcgtg ctgccctcga acgagggtcg cggttacgtg ttgcggcgca tcctgcgccg
    15781 cgcctgccgc catggccaca agctcggcgc gcaggatacg ttcttccaca agctggtcgg
    15841 cccgttggcc gaggccatgg gcgatgccta cccggaactg cacgagaagc gcgcgcagat
    15901 cgagaaggtc ctgctgctcg aggaagagca gttcgcgcgt acgctcgaca ccggcatgcg
    15961 catcctcgag caggacatcg cggcgttggc gggcgacacg ctcgacggtg acaccgtgtt
    16021 caagctctac gacacgttcg ggtttccgat ggacctcacg gcggacatcg cgcgcgagcg
    16081 cggcctgctc gtggacgagg aaggcttcga gcgcgcgatg caggcacagc gcgagcgtgc
    16141 gcgcggcgcc agccacttcg ccgccgagca gtacggtgac gcgggcatcg acacgcccac
    16201 cgtgttcacc ggctacgagg cgcttgcagg cgaggcgcgc gtgctggcgc tgctgcgcga
    16261 cgggcagcgt gtcgagggcc ttgccgaggg cgagaagggc atcgtggtgc tggaccgcac
    16321 gccgttctac gccgagtccg gcggccaggc cggtgatgcc ggcgtgatcg aggccggggg
    16381 ctggcgcttc gaggtgcgcg acacgacaaa ggcgggcgcg atctcgctac accacggcgt
    16441 catggcgcgc ggcacgctgg ccgttggtgc cgctgccgat gcgcgcgtgg agcccggcat
    16501 ccgccaggcc acggcgctga accactccgc cacgcacctg ctgcacgccg cactgcgcca
    16561 ggtgctgggc gagcatgtcg cgcagaaggg ctcgctggtc gacgccgagc gcctgcgctt
    16621 cgacttctcg cacttcgagg cgatgacgcc cgtgcagatc cgcgcggtcg agcgcctggt
    16681 gaacgagcag atccgcgcca actccgcggt cgagatccag ctcaccgaca tggacaccgc
    16741 gaaggcgaag ggcgcgatgg cactgttcgg cgagaaatac ggtgacgagg tgcgcgtgct
    16801 cacgatgggc ggcggcttct cggtggagct ctgcggcggc acgcacgtgc agcgcaccgg
    16861 cgatatcggc ctgatgcgcg tgaccgcgga atccggcatc gcggccggcg tgcgccgcat
    16921 cgaggcggtc acgggcgcca atgcgctcgc gctgttcgac acgctggagg cgcgcggcga
    16981 tcgcatcgcg cagctgctga agtccgggcg cgacaacgcg gtcgagaagg tcgagcaact
    17041 gatcgccgcg aaccgccagc tcgagaagga gctggagaaa ctgcgcgtgc agctcgcgag
    17101 cggcacgggc agggatctcg cggccggcgc gatcgacgtg aacggcgtga aagtgctggc
    17161 agcgcaactc gagggcgctg acgcgaaaag cctgcgtacc acgatggacc agctgaaaga
    17221 caagctcggc acggccgtga tcctgctggc agcggtcgag ggcgacaagg tctcgctggt
    17281 ggccggtgtc acgggcgggc tgcaggagcg cgtgcgtgcg ggcgacctga tggccgagat
    17341 cgcaccgctg ctcggcggca agggcggcgg gcgtgccgac atggcccagg gcggcggcac
    17401 cgatgccgcg gcgctgccac aggcgctgca gcgcgtgccc gaatgggtgc gcgcacgcct
    17461 ggcctagccg cgagccgggc gggggaattc gcctggatcg ccgggagtac gagcgagtcg
    17521 cagttttcgc ttgggaatcc gggaaggatt ctctataatc ccgcgctccc gcgggatggc
    17581 gtagctccag ggaggccggc aggacagcgg ccgaggagcg caagcggctc aaatgagagg
    17641 gacaccatgc ttatcttgac gcgccgggta ggtgagacgt tgatgatcgg cgacagcgtg
    17701 accatcacgg tgctgggagt gaaaggcaac caggtgaggc tcggcgtgaa tgccccgaag
    17761 gacgtatcgg tgcaccgcga ggagatctac cagcgcatcc agcgcgagca cgacggcgag
    17821 gacagcgaac cctcgagcta gcccgtaggt ccggcttcag tcggacagga tgtaggtccg
    17881 gcttcagccg gacatgattt tgaagagttc cggagagatg gccgagtggc tgaaggcgcg
    17941 cccctgctaa gggcgtatag gctaatcccc tatcgagggt tcgaatccct ctctctccgc
    18001 caattcc
//
LOCUS       DAORUQ010000050        17704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2857_length_17704_cov_5.909797, whole genome shotgun sequence.
ACCESSION   DAORUQ010000050 DAORUQ010000000
VERSION     DAORUQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2857_length_17704_cov_5.909797"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..711)
                     /gene="hflD"
                     /locus_tag="PLW13_06345"
     CDS             complement(82..711)
                     /gene="hflD"
                     /locus_tag="PLW13_06345"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HQY70027.1"
                     /translation="MNAADAARSQAIALAAVLECAILVDQLARTGAAPPEEMRALTDS
                     LFRVEWDAVEDVFGGVDNLARGLQHLEAMVAPVPGIEQGPVLRYAMSLLHLGKRLRGD
                     RERLAIIRSRLQHTGLKSEHFATSYDELAASMAAIYQDTISTYRLRIQVAGSAQYLQD
                     ERVAARIRALLLAGVRAAVLWRFVGGNRLGLLLGRGRLREACRALRRKP"
     gene            complement(708..1799)
                     /gene="mnmA"
                     /locus_tag="PLW13_06350"
     CDS             complement(708..1799)
                     /gene="mnmA"
                     /locus_tag="PLW13_06350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003174710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQY70028.1"
                     /translation="MSLGERVIVGISGGVDSAVSALLLREQGYAVEGLFMKNWDEDDG
                     TEYCTARADLADAEAVCALLGIRLHTANFAAEYWDNVFEHFLAEYRAGRTPNPDVLCN
                     REIKFKVFVEYAEALGATRIATGHYARMALAVGQPRLLKGLDAGKDQSYFLHAVPGEQ
                     LAKCLFPVGELPKAEVRRIAGEHAFPVATKKDSTGICFIGERRFGDFLRRYLPAQPGI
                     MRSVDGEPLGEHHGLMYYTIGQRQGLGIGGRRDHAEEPWYVADKDLANNALIVAQGSE
                     HPALFHRALGCGQLHWIGPPPAPTPLRLHAKIRYRQADQACTLLAAPGGRWRVEFEQP
                     QRAVTPGQYVVFYDGDCCLGGGVIEERFA"
     gene            complement(1796..2260)
                     /locus_tag="PLW13_06355"
     CDS             complement(1796..2260)
                     /locus_tag="PLW13_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY70029.1"
                     /translation="MSDRHAHWIPHVTVATVVFRDGRYLLVEEIDGGRRVLNQPAGHL
                     EPNETLVEAARRETLEETGWEVEIQALLGASLFTSAQTGVSYFRTSFLAQALHHHAGR
                     ALDAEIVAPRWLSYEEILAERARLRSESVLATIEQHRAGHRFPLHFFYPQAS"
     gene            complement(2287..2892)
                     /locus_tag="PLW13_06360"
     CDS             complement(2287..2892)
                     /locus_tag="PLW13_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY70030.1"
                     /translation="MPDVILFNKPWGVLSQFSDREGRCTLADFVHAPGFRPAGRLDYD
                     SEGLLVLCDDGVLQARIASPRFKLAKTYLVQVEGEISDAALARLRRGVMLNDGPTQPA
                     GAERIAAPPLWERDPPVRFRASIPTSWLSLTLTEGRNRQVRRMSAAVGFPTLRLVRWR
                     IGPWSLEGLAPGAWRREAVNLPAQAKKATRRPAPRRPRPRL"
     gene            3011..4267
                     /gene="icd"
                     /locus_tag="PLW13_06365"
     CDS             3011..4267
                     /gene="icd"
                     /locus_tag="PLW13_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQY70031.1"
                     /translation="MGYKHIKVPAQGEKITVNADFTLNVPANPVIPFIEGDGIGVDIT
                     PAMLKVVDAAVAKAYGGKRKISWMEIYTGEKAAELYDGDWFPAETLDAIREYVVSIKG
                     PLTTPVGGGFRSLNVALRQELDLYVCLRPVRWFSGVPSPVREPGKTNMVIFRENSEDI
                     YAGIEWKAGSADADKVIKFLREEMGVTKIRFPENCGIGIKPVSKAGTERLVRRAIQYA
                     IDQDLPSVTIVHKGNIMKFTEGGFKEWGYALAQNEFGGELLDGGPWVKLRNPKTGKDI
                     VIKDVIADAMLQQVLLRPDEYSVIATLNLNGDYLSDALAAQVGGIGIAPGANLSDTVA
                     LFEATHGTAPKYAGQDKVNPGSLILSAEMMLRHMGWNEAADLIISGMNGAIQARTVTY
                     DFERLMEGATLLKCSQFGDAIIKHMA"
     gene            complement(4310..4549)
                     /gene="cspD"
                     /locus_tag="PLW13_06370"
     CDS             complement(4310..4549)
                     /gene="cspD"
                     /locus_tag="PLW13_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HQY70032.1"
                     /translation="MRTGVVKWFNNAKGYGFILADECSSDVFAHYSAIRMEGYKTLKA
                     GQTVRFEVEAGPKGMHAIAIEALEALEDAAAMLMK"
     gene            4788..5141
                     /gene="clpS"
                     /locus_tag="PLW13_06375"
     CDS             4788..5141
                     /gene="clpS"
                     /locus_tag="PLW13_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HQY70033.1"
                     /translation="MDIRLASRDGKGAPKGDGDLALQEAKPELKQPPMYQVVMINDDY
                     TPMEFVVEVLQLFFGMGREKATQVMLAVHTQGKAVCGVYTRDIAETKAMQVNRYAREN
                     QHPLLCEIEAAGDAP"
     gene            5212..7467
                     /gene="clpA"
                     /locus_tag="PLW13_06380"
     CDS             5212..7467
                     /gene="clpA"
                     /locus_tag="PLW13_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HQY70034.1"
                     /translation="MLSKDLELNLNAAFKGARARRHEFMTVEHLLLALLDNDAAGKVL
                     RACGADMIQLRGELAEFVETTTPVIPQEDVERETEPTLGFQRVLQRAVFHVQSSGRNE
                     VTGANVLVALFSEQESQAVYLLKKQNIARIDVVNYITHGISKVSDAPRPGGAEPGEEE
                     QQGQDGEASPLQSFATNLNDEARAGRIDPLVGRAAELERVAQILARRRKNNPLLVGES
                     GVGKTAIAEGLAKRIVDGDVPDVLRTSVVYSLDLGSLLAGTKYRGDFEKRLKGLLAEL
                     KKKKHAILFIDEIHTIIGAGAASGGVMDASNLLKPLLSSGDIRCIGSTTFQEYRGVFD
                     KDRALSRRFQKIDVGEPSVAETVEVLKGLRSRFEEHYGLRYSTKALQVAAELANRYIT
                     DRFLPDKAIDVIDEAGAFQRLQPESRRKKVIGVKEVEDIVAKIARIPPRSVSASDKES
                     LRTLDADLKQVVFGQDEAMDALSTSIKLSRAGLKNVEKPIGCFLFAGPTGVGKTEASR
                     QLAKTLGLELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVTKHPHCVLLLD
                     EIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNTVLIMTTNAGAEMLSRRSIGFTEQ
                     DNSTDGLEAVKKVFTPEFRNRLDGIIQFGALSPEVVRSVVRKFLAELEAQVAERKVRI
                     EIDEAAQDWLVAKGYDEKMGARPMARVIQEYIKKPLAEILLFGELEHRPGVVHVTVEG
                     DGLALRVEPLH"
     gene            complement(7515..7733)
                     /gene="infA"
                     /locus_tag="PLW13_06385"
     CDS             complement(7515..7733)
                     /gene="infA"
                     /locus_tag="PLW13_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQY70035.1"
                     /translation="MAKEDVLEMEGEVVDTLPNTTFRVRLENGHIVTAHISGKMRKHY
                     IRILTGDKVKVELTPYDLTKGRITFRER"
     gene            complement(7867..8586)
                     /locus_tag="PLW13_06390"
     CDS             complement(7867..8586)
                     /locus_tag="PLW13_06390"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HQY70036.1"
                     /translation="MGNSVTLLANVRVFATYPHNCSYLAGEQATTLFVDPSTPIDARI
                     YSQLSYIGFRRSGPHLYRPHCAACNACVPARVPVEAFAPNRSQRRILARNADLEARVV
                     ESINSDAHFALYSRYIEQRHRDGDMYPPDREQYDSFLTREWGSTSFVEFRAAGRLLAV
                     AVMDRLENGLSAIYTYYEPAEEKRSLGSFVILWQIERARALALPAVYLGYWIRNCRKM
                     NYKTRFRPIELLANGHWVRVD"
     gene            complement(8571..9296)
                     /gene="aat"
                     /locus_tag="PLW13_06395"
     CDS             complement(8571..9296)
                     /gene="aat"
                     /locus_tag="PLW13_06395"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQY70037.1"
                     /translation="MSSIAWLTADVEAFPPLARALAEPDGLLAAGGDLSPARLIAAYR
                     RGIFPWYEEPEPILWWSPDPRAVLFPGELHLGASLRRTLRRRLYTVSADRCFAAVIGA
                     CAAPRARTAGTWIGAGMRAAYCELHRLGHAHSIETWRDGELVGGLYGIALGRVFFGES
                     MFSRADDASKVALAHLVRQLRAWGFALIDCQQDTAHMRRLGAQTISRARFRDILDANV
                     DLPGRPGPWHLDWSDGEEAWVTP"
     gene            complement(9305..10255)
                     /gene="trxB"
                     /locus_tag="PLW13_06400"
     CDS             complement(9305..10255)
                     /gene="trxB"
                     /locus_tag="PLW13_06400"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQY70038.1"
                     /translation="MADSIHHRLIILGSGPAGYTAAVYAARANLAPALITGIQQGGQL
                     MTTTEVENWPGGHAHLQGPELMEAMREHVTRFEATLIADHIASADLAARPIVLRGDSG
                     TYTCDALIIATGASARYLGLPSEEKFLGRGVSACATCDGFFYRGKTVAVIGGGNTAVE
                     EALYLSNLAAKVYVVHRRDALRSEKILQQRLLARATEGKVEMLWNHTLDEVLGDATGV
                     TGMRIRATADGATREVALDGVFVAIGHSPNTEMFAGQLEMHNGYIRTRSGLEGNATAT
                     SVAGVFAAGDVADHVYRQAITSAGFGCMAALDAEKYLDSQ"
     gene            10481..12913
                     /locus_tag="PLW13_06405"
     CDS             10481..12913
                     /locus_tag="PLW13_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQY70039.1"
                     /translation="MPPILQHGLREGLMIAWVALAAYLLLAIGTYSVGDPGWSSTGNG
                     AGISNSAGSAGAWIADVLLSLFGYVAYLFPVLLGFRAWALVRDRQSPFVFDWLLVSIR
                     SVGFVLVVLAGTGLLAIKGSATTLLPFGIGGMLGETLAQVCQRAFGLPGSQLLMVALL
                     LFGVTIFTDLSWGRLLETTGRLTLAALGRLAPLAGAAIARMPRGEPAGEQDDQPGSSP
                     GVAAVARELTADELEESSPRRRRARREPVLDDVPMDLPATPGMRVTPEMSDAPQSRTT
                     PEIRLPEKRKESQGERLQREKQGALFSAAGTTLPPLGLLDMADRSKQRGFSAEALEAM
                     SRLLEIKLRDFGVEAKVVSVLPGPVITRFEIQPAPGVKVSRISNLVKDLARSLAVISV
                     RVVEVIPGKSVVGIEIPNEDRETVLLGDVLASRAYEASKSPLTLALGYDISGEPMVVD
                     LARMPHLLVAGTTGSGKSVGVNAMLLSLLYKSTPADVRLIMVDPKMLELSVYEGIPHL
                     LTPVITDMKDAANGLRWCVAEMERRYKLMAAMGVRNLAGFNRKVLDAIEAGEPIADPF
                     WTPDPMVFITEEEQLATPPTLEALPAIVVVIDEFADMMMIVGKKVEELIARIAQKARA
                     AGIHLVLATQRPSVDVITGLIKANIPTRMAFQVSSKIDSRTILDQGGAEQLLGHGDML
                     YLPPGSGVPIRVHGAFVSDAEVHRVVANLRANSSPNYIDGILEESASSPLALLPGEAS
                     EDPEGDGLYDEAVRFVTESRKASISAVQRKLRIGYNRAARMIEAMEAAGVVSAMNSNG
                     SREVLAPPPPAH"
     gene            12932..13555
                     /gene="lolA"
                     /locus_tag="PLW13_06410"
     CDS             12932..13555
                     /gene="lolA"
                     /locus_tag="PLW13_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQY70040.1"
                     /translation="MSRIAALAALVLLCSHALADSAAATRLGALLAGMQRIEAGFSQR
                     LIDEGGEVMQESSGRVLLAHPGRFRWETTEPFEQLVVSDGATVWQYDADLAQVVVRPL
                     DRRADQVPSLLLSGEIAAVEKQFEIRSAAAPAGKERFDLLPREPGGLFAALAVQFRAG
                     VLEQLVIADGLGQRTEVEFSEVQPAGAVDDATFRFEAPPGVDELHDD"
     gene            13548..14891
                     /locus_tag="PLW13_06415"
     CDS             13548..14891
                     /locus_tag="PLW13_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQY70041.1"
                     /translation="MTDPAATAPYVPLAARMRPRRPEDFFGQEHLLGPDKPLSNAIRS
                     GVVHSMILWGPPGVGKTSLARMLAETTGAHFQSISAVLSGVKEIRAAIDGARAERAAS
                     GRRSILFVDEVHRFNKTQQDAFLPFVEDGTVIFIGATTENPSFELNGALLSRARVYRL
                     RPMSEEAIEAVLAHALVNDEPLRARGLAVEPLELGRIARAADGDARRALNLLEIAADL
                     AGAGVIDDAVLREVLGGDPRRFDKGGDIFYDQISALHKAVRGGSPDATLYWLARMLDG
                     GCDPLYIARRVVRMATEDIGNADPRGLTMALAAWDTQERLGSPEGELAIAQALAYLAC
                     APKSNAVYTAFGAARAEVQQSPSYEVPLHLRNAPTRLMKEMGFGGEYRYAHDEEDAYA
                     AGENYFPPELATRRYYHPVPRGLEKQIADKLDSQRARDAQSPRQRYPGASRPRKG"
     gene            14901..15296
                     /gene="crcB"
                     /locus_tag="PLW13_06420"
     CDS             14901..15296
                     /gene="crcB"
                     /locus_tag="PLW13_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQY70042.1"
                     /translation="MTDLLRNMLFIALGGALGALARFGAANLVHLLWPSRFPFATLAI
                     NVSGSLAIGVLYVLIAERAVMHADWRSIAMVGFLGAYTTFSTFSLESVTLIENGFVVQ
                     ALGYMLGSVCLCVGGAWAGIVLARLLWTP"
     gene            15307..16602
                     /gene="serS"
                     /locus_tag="PLW13_06425"
     CDS             15307..16602
                     /gene="serS"
                     /locus_tag="PLW13_06425"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQY70043.1"
                     /translation="MIDAKTLRQDAEDVARALARKRFTLDIAQYRALEERRKGCDVES
                     QGLQAARNQASRRIGELVREGVPVDEAKARAGQEMAEINSALERVREEGEVIHAELDA
                     FLAAIPNLPDADVPEGASEEHNVEVLRWGTPRVFDFPVRDHVDLGARGAAMDFDAGAR
                     LTGSRFVVLRGEIARLHRALIQFMLDLHTAEHGYEELNVPYMVNADALFGTGQLPKFA
                     EDLFRLGIDEGYYLIPTAEVPVTNLARDAIVEDAAALPRRMVCHTPCFRSEAGSYGKD
                     QRGMIRQHQFEKVELVQLVRPDQSAAALEELTGHAEAVLRRLGLPYRKIVLCGGDMGF
                     AARKTYDLEVWLPGQDKYREISSCSNMGDFQARRMLARWRNPETGKPELLHTLNGSGL
                     AVGRTLVAILENCQNADGSVTVPAVLRPWMNGAASILNP"
     gene            16611..>17704
                     /gene="cysG"
                     /locus_tag="PLW13_06430"
     CDS             16611..>17704
                     /gene="cysG"
                     /locus_tag="PLW13_06430"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HQY70044.1"
                     /translation="MDYLPVFLDVRGRDALVVGGGKVALRKAELLVQAGALPRVVAPA
                     IQPALVELAESHGGSCDAVPFAPRHLSGAALVIVATGVPEVDAAVHREAGARGVPVNV
                     ADRPELCTFILPAVVDRSPVIVAFSTGGRVPVLARSLRARLEAMLPAGLGRLADYLGA
                     KRGWLRERVPDPGLRLRAWERLLEGAVAERVLAGDEAGAERLLQEEVAALDGAPRGEV
                     FLVGAGPGDPDLLSFRALRLMQQADVVFYDRLVSAPVLALVRREAERVYVGKRRGEHA
                     VAQPDINELLAEHARRGRRVLRLKGGDPFIFGRGGEEIEHLAEAGIPFQVVPGITAAS
                     GCAAYAGIPLTHRDHAQSVRFVTGHLKDGS"
BASE COUNT         2740 a         5951 c         6284 g         2729 t
ORIGIN      
        1 atacggcatt catgtcgact ggtgcctgcg ggcatgcggc gggttcggct ttgccgaacc
       61 cttgtcgggc tgaagcccga cctacggttt gcgacgcagc gcgcgacagg cctcgcgcag
      121 ccggccgcgc ccgagcagca gtcccagccg gttgccgccg acgaagcgcc agagcaccgc
      181 ggcgcgcacg cccgcgagca gcagggcgcg gatgcgcgcc gcgacgcgct cgtcctgcag
      241 atactgcgcg ctgcccgcga cctggatgcg caggcggtag gtgctgatgg tgtcctggta
      301 gatcgcggcc atgctggcgg cgagctcgtc gtagctggtc gcgaagtgct cggacttgag
      361 ccccgtgtgc tgcaggcggg aacggatgat cgcgagtcgc tcgcggtcgc cgcgaagccg
      421 cttgcccagg tgcagcagcg acatcgcgta gcgcagcacg gggccctgct cgatgcccgg
      481 gaccggcgcg accatcgcct cgaggtgctg caggccgcgc gcgagattgt ccacgccgcc
      541 gaatacgtct tccaccgcgt cccactcgac gcggaacagg ctgtcggtca gcgcgcgcat
      601 ctcctcgggc ggcgcggcgc cggtgcgcgc gagctggtcg accaggatcg cgcactcgag
      661 cacggcggcg agcgcgatgg cctgtgagcg cgcggcgtcg gcggcattca tgcgaaacgc
      721 tcctcgatga cgccgccgcc gaggcagcag tcgccgtcgt agaacacgac gtactgcccg
      781 ggcgtcaccg cgcgctgggg ctgctcgaat tccacgcgcc agcgcccgcc gggtgccgcg
      841 agcagggtgc acgcctggtc ggcctggcga tagcggatct tggcgtgcag gcgcagcggc
      901 gtcggtgcgg gcggcggccc gatccagtgc aactgcccgc agccgagcgc gcggtgaaag
      961 agcgccgggt gctcgctgcc ctgcgccacg atcagcgcgt tgttcgcgag atccttgtct
     1021 gccacgtacc acggctcctc ggcgtggtca cggcgtccgc cgatgccgag gccctggcgc
     1081 tggcctatgg tgtagtacat caggccgtgg tgttccccga gcggctcgcc gtcgacgctc
     1141 cgcatgatcc ccggctgcgc cggcaggtag cggcgcagga agtcgccgaa gcgccgctcg
     1201 ccgatgaagc agatgccggt gctgtccttc ttggtcgcga ccgggaaggc gtgctcgccg
     1261 gcgatgcgcc gcacctcggc cttgggcagt tcgccaaccg ggaacaggca cttggccagt
     1321 tgttcgccgg gcaccgcgtg caggaagtag ctctggtcct tgccggcatc gagacccttc
     1381 agcaaccgcg gctggccgac ggcgagcgcc atgcgcgcgt agtgccctgt cgcgatgcgc
     1441 gtcgcaccca gcgcctcggc atactcgacg aagaccttga acttgatctc gcggttgcac
     1501 agcacgtcgg ggttcggcgt gcgcccggcg cggtattcgg ccaggaagtg ctcgaacacg
     1561 ttgtcccagt actcggcggc gaaattggcc gtgtgcaggc ggatgccgag cagcgcgcac
     1621 accgcctcgg cgtcggcgag gtcggccctg gcggtgcagt actcggtgcc gtcgtcctcg
     1681 tcccagttct tcatgaacag gccctcgacc gcgtagccct gctcgcgcag cagcagcgcg
     1741 gaaaccgccg aatcgacgcc gcccgagatg ccgacgatga cgcgctcgcc caggctcatg
     1801 aggcctgggg atagaaaaaa tgcagcggga agcggtgccc ggcgcggtgt tgctcgatgg
     1861 tcgccagcac cgattcgctg cgcaggcgag cgcgctcggc gagtatctcc tcgtagctca
     1921 gccagcgcgg ggcgacgatc tcggcgtcga gcgcacgccc ggcgtgatgg tgcagggcct
     1981 gcgcgaggaa gctggtgcgg aaatagctga cgccggtctg cgcgctggtg aacaggctgg
     2041 cgcccagcag cgcctggatc tcgacctccc agccggtctc ctcgagggtt tcgcgccgcg
     2101 cggcctcgac cagcgtctcg ttgggctcca ggtgccccgc gggctggttc agtacccgtc
     2161 gcccgccgtc gatctcctcg accagcaggt agcggccatc gcggaacacc accgtggcca
     2221 cggtgacgtg cggtatccaa tgcgcatgcc ggtcgctcat cgtcgggtcc cgtcaaaagg
     2281 cgcgcattat agccgcgggc gcgggcggcg cggggcaggg cgccgtgtgg cctttttcgc
     2341 ttgcgcgggc aggttgacgg cttcccggcg ccatgcgccg ggggcgagcc cttccaggct
     2401 ccacgggccg atgcgccagc gcaccaggcg cagcgtcggg aagccgaccg ccgcgctcat
     2461 gcgccgtacc tggcggttgc ggccctcggt gagcgtgagg ctgagccacg aggtcgggat
     2521 gctggcgcga aaacgcaccg gcggatcgcg ctcccacagc ggcggggcgg cgatgcgctc
     2581 ggcgccggcc ggctgcgtgg ggccgtcgtt cagcatcacc ccgcggcgca gccgggccag
     2641 cgccgcatcg ctgatctcgc cctcgacctg caccaggtag gtcttggcca gcttgaagcg
     2701 cggcgaggcg atgcgcgcct gcagcacgcc gtcgtcgcag agcacgagga gtccctcgga
     2761 gtcgtaatcg agccgtccgg cgggccggaa gccgggcgcg tgcacgaaat ctgccagcgt
     2821 gcaccgtcct tcccggtcgc tgaactggct cagcacgccc cagggcttgt tgaacaggat
     2881 cacatcgggc acggggctgg ctcgctcgct gtctgtccgg gggcgctgca gcgcggggaa
     2941 gcgatggtat actgccgcgc ctcgcaagag accggctatt cattccctgg ccacttcact
     3001 ggagcaatca atgggttaca agcacatcaa ggtaccagca caaggcgaaa agatcaccgt
     3061 caacgcggat ttcacgctga acgtgcccgc caatccggtc attcccttca tcgagggcga
     3121 cgggatcggc gtcgatatca cgccggccat gctgaaggtc gttgatgcgg cggtcgccaa
     3181 ggcctacggc ggcaagcgca agatcagctg gatggaaatc tacaccggcg agaaagccgc
     3241 cgagctctac gacggcgact ggttccccgc cgagacgctg gacgccatcc gcgagtacgt
     3301 ggtctcgatc aagggcccgc tgaccacccc ggtgggcggc ggcttccgct cgctgaacgt
     3361 ggcgctgcgc caggagctcg atctctacgt ctgcctgcgc ccggtgcgct ggttcagcgg
     3421 cgtgccctcg ccggtgcgcg agcccggcaa gaccaacatg gtgatcttcc gcgagaactc
     3481 cgaggacatc tacgccggca tcgagtggaa ggccggctcg gccgatgccg acaaggtgat
     3541 caaattcctg cgcgaggaaa tgggcgtcac caagatccgt ttccccgaga actgcggtat
     3601 cggcatcaag ccggtgtcga aggccggcac cgagcgcctg gtgcgccgcg cgatccagta
     3661 cgccatcgac caggacctgc cctcggtcac catcgtgcac aagggcaaca tcatgaagtt
     3721 caccgagggt ggcttcaagg aatggggcta cgcgctggcg cagaacgagt tcggtggcga
     3781 gctgctcgac ggcggcccgt gggtgaagct gcgcaacccg aagaccggca aggacatcgt
     3841 gatcaaggac gtgatcgccg acgcgatgct gcagcaggtg ctgctgcgcc ctgacgagta
     3901 cagcgtgatc gccacgctga acctcaacgg cgactacctc tccgacgcgc tggccgcgca
     3961 ggtgggcggc atcggcatcg cgcccggggc caacctgagc gacaccgtgg cgctgttcga
     4021 ggccacccac ggcacggcgc ccaagtacgc cggccaggac aaggtgaacc ccggctcgct
     4081 gatcctgtcg gcggagatga tgctgcgcca catgggctgg aacgaggccg ccgacctgat
     4141 catcagcggc atgaacggcg cgatccaggc caggacggtg acctacgact tcgagcgcct
     4201 gatggaaggg gcgaccctgc tgaagtgctc gcaattcggt gacgcgatca tcaagcacat
     4261 ggcgtgaccg ggcggaccgg cagccgccgt caggcgctgc cggtccactt catttcatca
     4321 acatggccgc cgcatcctcg agcgcctcga gcgcttcgat ggcgatcgcg tgcatgccct
     4381 tgggtcccgc ttcgacctcg aagcgaaccg tctggcccgc cttcagcgtc ttgtagcctt
     4441 ccatccggat ggcggagtaa tgggcgaata cgtccgagct gcactcatcg gccaggatga
     4501 agccgtagcc cttggcgttg ttgaaccact tgacgacacc agtacgcatg aatctggtcc
     4561 tcgctgcccg ggacgggccg gcggattgtt gtctttatgt tcctgccgtc cacggctggt
     4621 gctcgggggc attgcgagcg ttttaaacga cacgcaatcc aatagtcaag ccgtggtcgg
     4681 cagcgccgcc gcgtcgcatg ggccggcact tgtgcgcgac cgtgatccgt gaaacatttc
     4741 gccaacgtat cgagttttgt aaaacggcca tgaaccagat tttcgccatg gacatccgac
     4801 tggcctcgcg cgacggcaag ggagccccca agggcgatgg cgaccttgcg ctgcaggagg
     4861 ccaagcccga gttgaagcag ccgccgatgt accaggtggt gatgatcaac gacgactaca
     4921 cgccgatgga gttcgtggtc gaagtgctgc agctgttctt cggcatgggg cgggaaaagg
     4981 ccacccaggt catgttggcc gttcataccc agggcaaggc cgtctgcggc gtctacacgc
     5041 gcgacatagc cgagaccaag gcgatgcagg tcaaccgcta cgcgcgggag aaccagcacc
     5101 cgctgctgtg cgaaatcgag gccgcggggg atgcgccatg agccgtcgcc acgttgcggg
     5161 ctttctggac tatgctgaaa tcggagctgc ggttgctgcc ggaggtttgt catgctgagc
     5221 aaggatctgg agcttaatct caacgcggcc ttcaaggggg cgcgggcgcg tcggcacgag
     5281 ttcatgacgg tcgagcacct gctgctggcg ctgctggaca acgatgccgc gggcaaggtg
     5341 ctgcgggcct gcggcgcgga catgatccag ctgcgcggcg agctggcgga attcgtcgag
     5401 accacgacgc cggtgattcc gcaggaggat gtcgagcgcg agaccgagcc cacgctcggc
     5461 ttccagcgcg tgctgcagcg cgcggtgttt cacgtgcagt cctcgggccg caacgaggtc
     5521 acgggcgcca atgtgctggt cgcgctgttc agcgaacagg agagccaggc ggtatatttg
     5581 ctgaagaagc agaacatcgc gcgcatcgat gtcgtcaact acatcaccca tggcatttcc
     5641 aaggtctccg acgcccctcg ccccggcggg gccgagccgg gcgaggagga gcagcagggc
     5701 caggacggcg aggcgagccc gctgcagagt ttcgcgacca acctcaacga cgaggcccgt
     5761 gccgggcgca tcgacccgct ggtggggcgt gccgcggagc tcgagcgcgt ggcgcagatc
     5821 ctcgcgcgcc ggcgcaagaa caatccgctg ctggtcggcg aatccggggt gggcaagacc
     5881 gcgatcgccg aggggctggc caagcgcatc gtcgacggcg acgtgcccga cgtgctgcgc
     5941 accagcgtcg tctactcgct cgacctgggt tcgctgctcg cgggcaccaa gtaccgcggc
     6001 gatttcgaga agcgcctcaa gggtctgctc gcggagctga agaaaaagaa gcacgcgatc
     6061 ctgttcatcg acgagatcca cacgatcatc ggcgccggtg ccgcctcggg cggggtgatg
     6121 gacgcctcca acctgctcaa gcccctgctc agctccggcg acatccgctg catcggctcg
     6181 accacgttcc aggaataccg tggcgtgttc gacaaggacc gcgcgctgag tcgtcgcttc
     6241 cagaagatcg acgtcggcga gccgagcgtc gccgagaccg tggaggtgct gaagggcctg
     6301 cgctctcgct tcgaggagca ctacgggctg cgctacagca cgaaggcgct ccaggtagca
     6361 gccgagctcg ccaaccgcta catcaccgac cgcttcctgc ccgacaaggc gatcgacgtc
     6421 atcgacgagg ccggcgcctt ccagcgcctg cagccggagt cgcggcgcaa gaaggtcatc
     6481 ggcgtgaagg aggtcgagga tatcgtcgcg aagatcgcgc gcatcccgcc gcgcagcgtc
     6541 tcggcctcgg acaaggaatc gctgcgcacg ctggacgccg acctcaagca ggtggtgttc
     6601 gggcaggacg aggcgatgga cgcgctctcg acctcgatca agctctcgcg tgccggcctg
     6661 aagaacgtcg aaaagccgat cggctgcttc ctgttcgccg gtcccaccgg ggtcggcaag
     6721 accgaggcga gccgccagct ggccaaaacc ctcggcctgg agctgatccg tttcgacatg
     6781 tccgagtaca tggaacgcca caccgtctcg cgcctgatcg gcgcgccgcc gggctacgtg
     6841 ggctttgatc agggcgggct gctgaccgat gccgtgacca agcacccgca ctgcgtgctg
     6901 ttgctcgacg agatcgagaa ggcacacccg gaagtgttca acctgctgct gcaggtgatg
     6961 gaccacggca cgctcaccga caacaacggg cgcaaggcgg atttccgcaa caccgtgctg
     7021 atcatgacca ccaacgccgg cgccgagatg ctgagccgcc gctcgatcgg cttcaccgag
     7081 caggacaaca gcaccgacgg gctagaggcc gtgaagaagg tgttcacgcc ggagttccgc
     7141 aaccgcctgg acggcatcat ccagttcggt gcgctcagcc ccgaggtggt gcgctcggtg
     7201 gtgcgcaagt tccttgccga gctcgaggcg caggtggccg agcgcaaggt gcgcatcgag
     7261 atcgacgagg cggcgcagga ctggctggtc gccaagggtt acgacgagaa aatgggcgcg
     7321 cggccgatgg cgcgcgtgat ccaggagtac atcaagaagc cgctcgccga gatcctgctg
     7381 ttcggcgagc tcgagcaccg ccccggcgtc gtgcatgtca ccgtggaagg ggacggactg
     7441 gcgctgcggg tggagccctt gcactgaatg cccgtgggtc cggatagatc cgggcccacg
     7501 gcttgcggcg agactcagcg ttcgcggaag gtgatacggc ccttggtgag gtcgtagggg
     7561 gtcagttcga ccttgacctt gtcgccggtg aggatgcgga tgtagtgctt gcgcatcttg
     7621 ccgctgatgt gcgcagtgac gatgtgcccg ttctcgaggc gcacccggaa cgtcgtattc
     7681 ggcagggtgt cgaccacctc gccttccatt tccaacacgt cttctttcgc catccagagc
     7741 cactcgagta aaaaatgagc cgcggcattc tgcctgatcg ggagggtgga agcaaagcgc
     7801 ctggtggccc gggatggcgc ggcagcatac ttttgcccaa atcggtgccg cggcgggccg
     7861 cgtgattcag tcgacgcgta cccagtggcc gttggcgagc agctcgatgg ggcggaaccg
     7921 cgtcttgtag ttcatcttgc ggcagttgcg gatccagtag ccgagataca ccgccggcag
     7981 ggcgagtgcg cgcgcgcgct cgatctgcca caggatcacg aagctgccga ggctgcgttt
     8041 ctcctcggcg ggttcatagt aggtgtagat ggcggacagc ccgttctcca gccggtccat
     8101 cacagcgacc gcgagcagcc ttcccgcggc gcggaactcg acgaagctgg tgctgcccca
     8161 ttcgcgcgtg agaaaggagt cgtactgctc gcgatccggc gggtacatgt cgccgtcgcg
     8221 atggcgctgc tcgatgtagc ggctgtagag cgcgaaatgc gcatcgctgt tgatggattc
     8281 cacgacccgg gcctcgaggt cggcgttgcg cgccaggatg cgtcgctggc tgcggttggg
     8341 ggcgaatgcc tccaccggca cgcgtgccgg cacgcaggcg ttgcatgcgg cgcaatgcgg
     8401 gcggtagagg tgcggcccgc tgcggcgaaa accgatgtac gagagctggc tgtagatgcg
     8461 cgcatcgatc ggcgtgctcg gatcgacgaa cagcgtcgtc gcctgctcgc ccgccaggta
     8521 gctgcagttg tgcggatacg tggcgaagac gcgcacgttc gccagcaggg ttacggagtt
     8581 acccatgcct cctcgccatc actccagtcc aggtgccagg gcccggggcg gcccggcagg
     8641 tcgacgttgg cgtctaatat atcgcgaaat cgcgcgcgcg aaatggtctg cgcgccgagg
     8701 cggcgcatgt gcgccgtatc ctgctggcaa tcgatcagcg cgaagcccca cgcgcgcagc
     8761 tgccgcacga ggtgcgccag tgcgaccttc gaggcatcgt cggcacggct gaacatcgat
     8821 tcgccgaaga acacgcgccc gagcgcgatc ccgtaaagtc cgccgaccag ctcaccgtcg
     8881 cgccaggtct cgatcgagtg cgcatggccg aggcggtgca gttcgcagta ggccgcgcgc
     8941 atgccggcac cgatccaggt gccggcggtg cgcgcgcgcg gcgcggcgca ggcgccgatc
     9001 accgcggcga agcagcggtc ggcgctcacc gtgtagagac gccggcgcag cgtgcgacgc
     9061 aggctcgctc cgagatgcag ctcgccggga aacagcaccg cgcgcgggtc cggcgaccac
     9121 cacaggatgg gctcgggctc ctcgtaccac gggaagatac cgcggcggta ggcggcgatg
     9181 aggcgcgcgg ggctgaggtc accgcccgcc gcgagcaggc cgtcgggctc ggcgagcgcg
     9241 cgcgccagcg gcggaaaggc ctccacgtcc gccgtgagcc aggcaatgga ggacatcggc
     9301 cgcgctactg cgagtcgagg tacttttccg cgtccagtgc cgccatgcag ccgaaaccgg
     9361 ccgaggtgat cgcctggcgg tagacgtggt cggccacgtc gccggcggcg aacacgcccg
     9421 cgacgctggt cgccgtggcg ttgccctcga gaccgctgcg ggtgcggatg tagccgttgt
     9481 gcatctcgag ctggccggcg aacatctccg tgttcggcga gtggccgatg gcgacgaaga
     9541 cgccgtcgag cgcgacctcg cgcgtcgcgc cgtccgcggt ggcgcggatg cgcatgccgg
     9601 tgacgcccgt ggcatcgccc agcacctcgt cgagcgtgtg gttccacagc atctcgacct
     9661 tgccttccgt cgcgcgcgcc agcaggcgct gctgcaggat cttctccgag cgcagcgcgt
     9721 cgcggcgatg caccacgtag acctttgccg cgaggttcga caggtacagc gcctcctcga
     9781 ccgcggtatt gccgccgccg atcacggcga ccgtcttgcc gcggtagaag aaaccgtcgc
     9841 aggtcgcgca cgcgctgacg ccgcgcccga ggaacttttc ctccgagggc aggccgagat
     9901 agcgcgccga ggcgccggtg gcgatgatca gcgcatcgca ggtgtaggtg ccggagtcgc
     9961 cacgcagcac gatcggtcgc gccgcgagat cggcgctcgc gatgtggtcg gcgatcagcg
    10021 tggcctcgaa gcgcgtgacg tgctcgcgca tcgcctccat caactcgggg ccctgcaggt
    10081 gcgcgtggcc acccggccag ttctcgacct cagtggtcgt catcaattgt ccgccctgct
    10141 ggatgcccgt gatcagcgcc ggcgcgaggt tcgcgcgcgc ggcatagacg gcggccgtgt
    10201 agccggccgg gcccgagccc aggatgatca ggcggtgatg gatgctgtcg gccatggtga
    10261 gcggttcctc cgggaattta cgcagttgtc gggtgccggg gcggtcgggg cagggcgccg
    10321 aaatgcgcct atactacggt gtggttccgc tgcggcgggc ggggcgcagt ctacccgcaa
    10381 cgaccgcggt ttcacaggat ggcctccgcg cgcggaggcc cgcagaggac cagagagctt
    10441 ggccaggaca cgaggcgcat cgacggtagc cgcaaggacg ctgcccccca ttctgcagca
    10501 cggcttgcgc gaggggctga tgatcgcctg ggtcgcgctg gcggcgtacc tgctgctcgc
    10561 catcggcacc tacagcgtcg gcgatccggg ctggtcgagc accggcaacg gcgccgggat
    10621 cagcaactcc gcgggctccg cgggcgcgtg gattgccgac gtgctgctct cgctgttcgg
    10681 ctacgtcgcc tacctgttcc cggtgctgct cggtttccgc gcctgggcgc tggtgcgcga
    10741 ccgccagtcg cccttcgtgt tcgactggct gctggtatcg atccgcagtg tcggctttgt
    10801 gctggtggtg ctcgccggca ccggtctgct ggcgatcaag ggcagcgcga ccacgctgct
    10861 gcctttcggc atcggcggca tgctcggcga aaccctggcg caggtgtgcc agcgcgcctt
    10921 cggcctgccg ggcagccagt tgctgatggt cgccctgctg ctgttcggcg tcaccatctt
    10981 taccgacctg tcgtgggggc ggctgctcga aaccaccggc cggctcacgc tggcggcgct
    11041 ggggcgcctg gccccgctgg ccggcgcggc gatcgcgcgc atgccgcgcg gggagcccgc
    11101 gggcgagcag gacgaccagc ccgggtcgag tcccggggtt gcagccgttg cgcgcgagct
    11161 cacggccgat gaactggaag aaagtagtcc gcgcaggcgc cgcgcccggc gcgaaccggt
    11221 gctggacgat gtgccgatgg acctgccggc cacgcccggg atgcgcgtga cgccggaaat
    11281 gagcgacgca ccgcagagcc gcacgacgcc ggagatccgc ctgcccgaaa aacgcaagga
    11341 atcgcagggc gagcgcctgc agcgcgaaaa gcagggtgcc ctgttcagcg ccgccggcac
    11401 gacgctgccg ccgctcggcc tgctcgacat ggccgacagg agcaaacagc gcggcttctc
    11461 ggccgaggcg ctcgaggcga tgtcgcggct gctcgagatc aagctgcgcg atttcggtgt
    11521 cgaggccaag gtggtctcgg tactgccggg gccggtgatc acgcgcttcg agatccagcc
    11581 cgcgcccggc gtgaaggtca gccgcatcag caacctggtc aaggacctcg cgcgctcgct
    11641 cgcggtgatc agcgtgcgcg tggtcgaggt gatcccgggc aagtcggtgg tcggcatcga
    11701 gatccccaac gaggaccgcg agacggtgct gctcggcgac gtgctcgcct cgcgcgccta
    11761 cgaggcctcg aagtcgccgc tgacgctcgc gctcggctac gacatctcgg gcgagccgat
    11821 ggtggtcgat ctggcgcgca tgccgcacct gctggtcgcg ggcaccaccg gttcgggcaa
    11881 gtcggtgggc gtcaacgcga tgctgctctc gctgctgtac aagtcgacgc ccgcggacgt
    11941 gcgcctgatc atggtcgacc cgaagatgct ggagctctcg gtctacgagg gcatcccgca
    12001 cctgctgacc cccgtgatca cggacatgaa ggacgccgcc aacgggctgc gctggtgcgt
    12061 ggcggagatg gagcgccgct acaagctgat ggcggcgatg ggcgtgcgca atctcgcggg
    12121 tttcaaccgc aaggtgctgg atgccatcga ggccggcgag ccgatcgccg atccgttctg
    12181 gacgcccgac cccatggtgt tcatcaccga ggaggagcag ctcgccacgc ccccgacgct
    12241 ggaggcgctg cccgcgatcg tggtggtgat cgacgaattc gccgacatga tgatgatcgt
    12301 cggcaagaag gtcgaggagt tgatcgcgcg catcgcgcaa aaggcgcgcg ccgccggcat
    12361 ccacctggtc ctcgccaccc agcgcccctc ggtcgacgtg atcaccggcc tgatcaaggc
    12421 caacatcccg acgcgcatgg ccttccaggt ctcctcgaag atcgattcgc gcaccatcct
    12481 cgaccagggc ggggccgagc agctgctcgg gcacggcgac atgctgtacc tgccgcccgg
    12541 cagcggcgtg ccgatccgtg tccacggcgc cttcgtctcc gacgccgagg tgcaccgcgt
    12601 ggtggcgaac ctgcgcgcga actcgagccc caactacatc gatggcatcc tcgaggagag
    12661 cgcctcctcg ccgctggcgc tgctccccgg ggaggccagc gaggatcccg agggcgacgg
    12721 cctctacgac gaggcggtgc gcttcgtgac cgagagccgc aaggcctcga tctcggcggt
    12781 tcagcgcaag ctgcggatcg gctacaatcg cgccgcccgg atgatcgagg ccatggaggc
    12841 cgcgggcgtg gtcagcgcca tgaacagcaa cggcagccgt gaagttctgg cccccccgcc
    12901 gcccgcccac tgacccactg caggtgttcc cgtgtcgcga attgccgctc tcgcagcgct
    12961 cgtcttgctg tgttcccacg cgctggccga cagcgccgcc gccacgcgcc tcggcgcact
    13021 gctcgccggc atgcagcgca tcgaggccgg gttctcgcag cgcctgatcg acgagggcgg
    13081 cgaagtgatg caggagagca gcgggcgcgt gctgctcgcg cacccggggc gctttcgctg
    13141 ggaaaccacc gagccgttcg agcagctcgt ggtcagcgac ggcgcgaccg tgtggcagta
    13201 cgacgccgac ctcgcgcagg tggtggtacg cccgctcgac cgtcgtgccg accaggtgcc
    13261 ctcgctgctg ctcagcggcg agatcgccgc ggtcgaaaag cagttcgaga tccgcagcgc
    13321 cgcggcgcct gccggcaagg aacgcttcga cctgctgccg cgcgagccgg gcgggctgtt
    13381 cgccgcgctg gccgtgcagt ttcgcgccgg ggtgctggag caactggtga tcgccgacgg
    13441 cctgggccag cgcaccgagg tggagttctc ggaggtgcag cccgcgggcg ccgtcgatga
    13501 cgccaccttc cgcttcgagg ctccacccgg ggtcgacgag ctgcacgatg actgatccgg
    13561 ccgcgaccgc gccctacgtg ccgctcgcgg cgcgcatgcg gccccggcgc ccggaagact
    13621 tcttcggcca ggagcacctg ctcggcccgg acaagccgct cagcaacgcg atccgctcgg
    13681 gcgtcgtgca ctcgatgatc ctgtgggggc cgcccggcgt cggcaagacc tcgctggcgc
    13741 gcatgctcgc cgagaccacc ggcgcgcact tccagagcat ttccgcggtg ctttccggcg
    13801 tgaaggagat ccgcgcggcc atcgacgggg cgcgcgccga gcgcgccgcg agcgggcggc
    13861 gcagcatcct gttcgtcgac gaggtgcacc gcttcaacaa gacccagcag gacgccttcc
    13921 tgccgttcgt cgaggacggc accgtgatct tcatcggcgc caccaccgag aacccgtcct
    13981 tcgaactgaa cggcgcgctg ctctcgcgcg cgcgcgtcta ccgtctgcgc cccatgagcg
    14041 aggaggcgat cgaggcggtg ctggcgcacg cgctggtgaa cgacgagccg ttgcgcgcac
    14101 gcggcctggc cgtggagccg ctcgagctcg ggcgcatcgc gcgcgcggcc gacggcgatg
    14161 cgcgccgcgc gctgaacctg ctggagatcg cggcggatct cgccggcgcc ggggtgatcg
    14221 acgacgcggt gctgcgcgag gtgctgggcg gcgatccgcg gcgcttcgac aagggcggcg
    14281 acatcttcta cgaccagatc tcggcgctgc acaaggcggt gcgcggcggc tcgcccgatg
    14341 cgacgctgta ctggctggcg cgcatgctcg acggcggctg cgaccccctc tacatcgcgc
    14401 ggcgcgtggt gcgcatggcc accgaggaca tcggcaatgc cgatccgcgc ggcctgacga
    14461 tggcactggc ggcctgggac acgcaggagc gcctcggcag cccggagggc gagctcgcga
    14521 tcgcgcaggc gctggcctac ctcgcctgcg cgccgaagag caacgcggtc tacacggctt
    14581 tcggcgccgc gcgcgccgag gtgcagcagt cgccgagcta cgaggtgccg ttgcacctgc
    14641 gcaacgcgcc gacgcgcctg atgaaggaaa tgggcttcgg cggcgagtac cgctacgccc
    14701 acgacgagga agacgcctac gcggccggcg agaattactt tccgcccgag ctcgccacgc
    14761 ggcgctacta tcacccggtg ccgcgcggcc tggagaagca gatcgccgat aagctcgaca
    14821 gccagcgcgc gcgcgatgcg cagagcccgc gccagcgcta tccgggcgcg agccgtccgc
    14881 gcaagggctg agggggagcg atgaccgacc tgctgcgcaa catgctcttc atcgcgctgg
    14941 gcggcgcgct gggcgcgctg gcgcgcttcg gtgccgcgaa cctggttcac ctgctgtggc
    15001 cctcgcgctt tccgttcgcg acgctggcga tcaacgtcag cggctcgctg gcgataggcg
    15061 tgctgtacgt gctgatcgcc gagcgcgccg tgatgcatgc cgactggcgc agcatcgcga
    15121 tggtgggctt tctcggcgcg tacaccacct tttccacctt ctcgctggag tcggtgacgc
    15181 tgatcgagaa cggtttcgtg gtgcaggcgc tcggctatat gctgggcagc gtgtgcttgt
    15241 gtgtgggcgg ggcgtgggcg ggtatcgtgc tcgcgcgcct gctgtggacc ccttgaggat
    15301 tgcggaatga tcgatgcaaa gaccttgcgc caggatgccg aggacgtcgc gcgggcgctc
    15361 gcgcgcaagc gcttcacgct ggatatcgcg cagtaccgcg cgctcgagga gcggcgcaag
    15421 ggttgcgacg tggagtccca gggcctgcag gcggcgcgca accaggcgtc caggcgcatc
    15481 ggcgagctgg tgcgcgaggg cgtgccggtc gacgaggcca aggcgcgcgc cgggcaggag
    15541 atggcggaga tcaacagcgc gctggagcgc gtgcgcgagg agggcgaggt catccacgcc
    15601 gagctcgatg ccttcctcgc ggcgatcccg aacctgcccg atgccgacgt gcccgagggt
    15661 gcgtccgagg agcacaacgt cgaggtcctg cgctggggca cgccgcgcgt cttcgatttc
    15721 ccggtgcgcg accacgtcga tctcggcgcg cgcggggcgg cgatggattt cgatgccggc
    15781 gcgcggctga ccggttcgcg cttcgtggtg ctgcgcggcg agatcgctcg gctgcaccgc
    15841 gcgctgatcc agttcatgct ggacctgcac accgccgagc acggctacga ggagctgaac
    15901 gtgccctaca tggtcaacgc cgatgcgctc ttcggcaccg gccagctgcc gaagttcgcc
    15961 gaggacctgt tccgcctcgg catcgacgag ggctactacc tgatcccgac ggccgaggtg
    16021 ccggtaacca acctcgcgcg cgatgccatc gtcgaggacg ccgccgcgct gccgcggcgc
    16081 atggtctgtc acacgccgtg ttttcgcagc gaggcgggca gctacggcaa ggaccagcgc
    16141 ggcatgatcc gccagcacca gttcgagaag gtcgagctgg tgcagctggt gcgccccgat
    16201 caatccgccg ccgcgctgga ggaactcacc gggcacgcgg aggcggtgct gcgccgcctc
    16261 gggctgccgt accgcaagat cgtgctctgc ggcggcgaca tgggtttcgc ggcgcgcaag
    16321 acctacgacc tcgaggtgtg gctccccggg caggacaagt accgcgagat ctcctcgtgc
    16381 agcaacatgg gtgatttcca ggcgcggcgc atgctggcgc gctggcgcaa tcccgagacc
    16441 ggcaagcccg agctgctgca cacgctaaac ggttccgggc tcgcggtcgg ccgcacgctg
    16501 gtcgcgatcc tggagaactg ccagaacgcc gacggcagcg tcaccgtgcc ggcggtgctg
    16561 cgaccctgga tgaacggtgc ggcctcgatc ctgaacccct gaggcccgcc atggactacc
    16621 tgccggtctt tctcgacgtg cgtgggcgcg acgcgctcgt cgtcggcggc ggcaaggtgg
    16681 cgctgcgcaa ggccgagctg ctggtgcagg ccggcgcgct gccgcgcgtg gtggcgccgg
    16741 ccatacagcc agcgctcgtg gaactggccg aaagccatgg cggcagctgc gacgccgtgc
    16801 ccttcgcccc gcggcacctc tcgggcgctg cgctggtgat cgtggccacc ggcgtgcccg
    16861 aggtcgacgc ggcggttcac cgcgaggccg gcgcgcgcgg cgtgccggtc aacgtggccg
    16921 accgtcccga gctctgcacg ttcatcctgc ccgcggtggt cgaccgttcg ccggtgatcg
    16981 tcgccttctc cacgggcggg cgcgtcccgg tgctggcgcg cagcctgcgc gcgcgcctcg
    17041 aggccatgct gcccgcaggg ctcggccggc tcgcggatta cctcggcgcc aagcgcggct
    17101 ggctgcgcga gcgcgtgccc gatcccggcc tgcgcctgcg cgcctgggag cggctgctcg
    17161 agggggcggt ggcagagcgc gtgctggcgg gcgacgaggc cggcgcggag cgtctgctgc
    17221 aggaggaggt ggcggcgctc gacggcgcgc cgcgtggcga ggtgttcctg gtcggcgccg
    17281 ggcccggcga tccggacctg ctcagtttcc gcgccctgcg cctgatgcag caggcggacg
    17341 tcgtgttcta cgaccgcctg gtcagcgcgc cggtgctcgc gctggtgcgc cgcgaggccg
    17401 agcgcgtcta cgtcggcaag cgccgtggcg agcacgcggt ggcgcagcct gacatcaacg
    17461 aactgctggc cgaacacgcg cgccgcggcc ggcgcgtgct gcgcctcaag ggcggcgatc
    17521 cgttcatctt cgggcgcggc ggcgaggaga tcgagcacct ggccgaagcc gggattccgt
    17581 tccaggtggt gccgggcatc accgccgcct cgggttgcgc ggcctatgcg ggcattccgc
    17641 tcacgcaccg cgaccacgcg cagtcggtgc gcttcgtgac cggtcacctg aaggacggca
    17701 gcat
//
LOCUS       DAORUQ010000051        17648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2873_length_17648_cov_6.345592, whole genome shotgun sequence.
ACCESSION   DAORUQ010000051 DAORUQ010000000
VERSION     DAORUQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_17648_cov_6.345592"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="guaA"
                     /locus_tag="PLW13_06435"
     CDS             complement(<1..799)
                     /gene="guaA"
                     /locus_tag="PLW13_06435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQY70045.1"
                     /translation="MNTDIHAQRILILDFGSQYTQLIARRVRELGVYCEIYSWRTPAA
                     AIKAFRPSGVILSGGPESVTGKSTPRVPAALFNMGIPLLGICYGMQAMAAQLGGRVEP
                     SNRREFGYAQVRVTASSRLLDGISDHVDEEGAARLDVWMSHGDKVVALPPGFELLAAT
                     DSAPVAAMCDESRGFYGIQFHPEVTHTHQGGRILEHFVRGICGCEGLWTPGSIIDDAI
                     ASIRKTVGKDKVLLGLSGGVDSSVVAALLARAIGDQLTCVFVDHGLLR"
     gene            complement(974..2443)
                     /gene="guaB"
                     /locus_tag="PLW13_06440"
     CDS             complement(974..2443)
                     /gene="guaB"
                     /locus_tag="PLW13_06440"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQY70046.1"
                     /translation="MLRVQEEALTFDDVLLVPGESEVVAKDVKLQTRLTRDITLNIPL
                     MSAAMDTVTESKLAIAIAQEGGIGIVHKNMTPEQQAEEVRAVKRFESGVVREPVTIDS
                     SRSIRDLIRLTIERNFSGVPVLNRKRELVGIVTSRDVRFATNLDEPVSSIMTRKRELV
                     TVLEGTDLADVKKLLHRHRIEKVLVVDDAFRLRGMITAKDIFKAERYPNACKDADGRL
                     RVGAAVGVGGGAADERVVRLIEAGVDVLVVDTAHGHSRNVRDTVRSIKSRYPQVQVIA
                     GNIATAEAAKYLLDAGVDGVKVGIGPGSICTTRIVTGIGVPQITAVANVAAVLEGTGV
                     PLIADGGIRFSGDLAKAVAAGAHVIMVGSMLAGTEEAPGEIELYQGRSYKAYRGMGSL
                     GAMAKGSSDRYFQNAAEGVEKLVPEGIEGRVPYKGPISAIVHQLMGGLRAAMGYTGSP
                     DIETMRTRPRFVRITSAGVNESHVHDVSITKEAPNYPVR"
     gene            complement(2614..3258)
                     /gene="msrA"
                     /locus_tag="PLW13_06445"
     CDS             complement(2614..3258)
                     /gene="msrA"
                     /locus_tag="PLW13_06445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQY70047.1"
                     /translation="MSTTPAIPMPADPLPGRAEPMTVPPLHFVNGARIVPPFPPGLEL
                     AEFALGCFWGAERRFWQQPGVYSTAVGYCAGLTPNPSYREVCSGRTGHTEAVLVVFDP
                     ARTSYPELLRVFWESHDPTQGMRQGNDIGTQYRSGIYTHSGEQLRLALASRDAYQQVL
                     RAAGFGPVTTEIRAAGPFYYAEEYHQQYLAKNPGGYCGIGGTGVACPVGAASGA"
     gene            complement(3299..4327)
                     /locus_tag="PLW13_06450"
     CDS             complement(3299..4327)
                     /locus_tag="PLW13_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY70048.1"
                     /translation="MSKAAPTDLPVEKLGAWLEQAVGDFHGLHTARKFAGGQSNPTFL
                     LEADSGRYVLRRKPPGALLASAHAVDREYRVLRALAPTDVPVPRALALCDDDTVIGSM
                     FYVMEFLDGRILWEPTLPELDAARRARVYDEMNRVLVALHSVDVGAVGLADYGQPGNY
                     YARQLNRWTKQYRAAETERIEAMEALMTWLAGRLPADDGRVVLVHGDYRLDNLMFDAT
                     EDRIVAVLDWELSTLGHPFADLAFQCMQWRLPHGAAALRGLGGVDRAALGIPREEEYV
                     ARYCAAMGIGAIPDWTFYLAISFFRLAAICQGVYKRGIDGNASSTEALRFGEAARMIA
                     SAGASVLD"
     gene            complement(4359..5057)
                     /locus_tag="PLW13_06455"
     CDS             complement(4359..5057)
                     /locus_tag="PLW13_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY70049.1"
                     /translation="MAELYLVRHAQASFHSADYDCLSALGERQSRWLGEYFASRDIRF
                     DVLVTGTLRRHRQTLAGILEGMELDTGGDAIELPGLDEYDFRALVAAFAVAHPDDARV
                     AAWRAEPATKPAFYRLLKLAMLAWAAGELPGPLPETWGDFMRRTTDAAAALQALTARG
                     ARLLAISSGGAMSALLGGRLGLSPAHIADLNMQVRNTSVSHFHLNRERFLLSSWNAVP
                     HLERAGREDAITYG"
     gene            5260..5757
                     /locus_tag="PLW13_06460"
     CDS             5260..5757
                     /locus_tag="PLW13_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70050.1"
                     /translation="MAQDAANIIEETVKPAARRVVRRAKAAATSKPAARKSAATKAAA
                     PKRAYKRKATAASKAKPAISLKKASETAKEVAYVQLGICGKVYDEVNTRVTKARKVAP
                     KKWSDLVKRGERVQQDLDKVQKDLTKDLRKRVNKLEIPAPIETRVTKFTKAVKKLTAR
                     VNKAA"
     gene            5871..6968
                     /locus_tag="PLW13_06465"
     CDS             5871..6968
                     /locus_tag="PLW13_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70051.1"
                     /translation="MRGNLDIAVIGGGINGISSATELAAEGHRVELFERGELMGETSS
                     RSSKLLHGGLRYLETGELRLVREALGERSRWLADCPQHAQPLRLALPVYAGVSRSPLL
                     LGAGLALYGLLAGRQGLGRSQWLGAREFARLNPQLQPDGLRGGFYFFDGRMDDRALGL
                     WLAGVARSRGVVIREHAPVLGIDAGGGVRLATGEQRFDLVVNAAGPWAEVLLRDSGLQ
                     PAHGIDWVRGSHIVFDEPLAQACLLQVPGEKRIFFVLPWQGRTLVGTTEVAQSREEPI
                     AASEAEIDYLLAARNHYFRAAKTRADVAEVFAGVRPLLHFEGDPGRASREYALARQDR
                     VLSIYGGKWTTARALGRQVARMVRESPPPPR"
     gene            complement(6940..7923)
                     /locus_tag="PLW13_06470"
     CDS             complement(6940..7923)
                     /locus_tag="PLW13_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HQY70052.1"
                     /translation="MRAMVLHAPGEPLRAEMRTVPAPGPEGVLLRVLACGICRTDLHV
                     VDGELREPRLPLVPGHQIVGEVVARGDRVKGFAVGERIGVPWLGGSCGDCDFCRAGEE
                     NLCDGAVYTGYQVDGGFAQYCVADARYCFAIPPGFDAVQAAPLLCAGLIGYRSYRPAR
                     AARRIGFYGFGAAAHILVQVARHHGQEVYAFTRPGDQEAQDFARRLGAVWAGDSSAPA
                     PVALDAAIIFAPEGELVPRALRAVRKGGRVICAGIHMSDIPSFAYADLWGERHIESVA
                     NLTRRDGEEFLPLAAALPVRTEVHRYALADVNRALDDLRAGRFTGAAVVIP"
     gene            8133..8696
                     /locus_tag="PLW13_06475"
     CDS             8133..8696
                     /locus_tag="PLW13_06475"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQY70053.1"
                     /translation="MLNPRILRLNVAGQPVEWLSWQEATCLYARDMVAWTLGDQVRTV
                     FGGMSRLTGLQSSIALHSIIACAGHVSCKPRLVPPLTNRALFRRDLHLCMYCGVRFGE
                     RELTRDHVLPKSRGGRDHWRNVVAACKRCNQRKGNRLPEEAGMEMLAVPYQPNTAEWL
                     ALINSDRIRGDQMEFLRTQFSPHSRWS"
     gene            8700..10184
                     /locus_tag="PLW13_06480"
     CDS             8700..10184
                     /locus_tag="PLW13_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70054.1"
                     /translation="MGIAIDRTDFSARDHANFARKLRQNLDALKSLLAQPGFGAGPSS
                     IGAEVELFIIDARGRPKPVNLEILARSGDPRLTLELNRFNLEYNLTPVAATGRPFSRI
                     EAQIGDALARVNAAAAPAQARVIPIGILPTLRARDFGPHAMTPQLRYQALAGALARSR
                     GERFAIRIGGPEPLRLVVEDVTLEGANTSLQIHYRVPPREFARSFNAIQLVTPVVLAS
                     ACNSPLLLGRRTWQETRVPLFKLAVDGRDRDSRALHLPPRVHFGTGWVREGAYELFSA
                     AVHLHEPLLPICSRENALSRLRAQRIPALDELRLHHGTLWPWNRPVYDPAHGGHLRIE
                     LRALPAGPSAADMMANAAFALGLARGLRDRIDDWLPGIPFAIAESNFYRAAEHGLDAT
                     LLWPDAHGTLARRNATDIARELLPVAARGLRLLGVHDAESAHYLGIMRTRLERRTNGA
                     TWQLAQLARLEARHGRSRALSLLVGHYARHALGNTPVAEWPDIE"
     gene            10181..11215
                     /locus_tag="PLW13_06485"
     CDS             10181..11215
                     /locus_tag="PLW13_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HQY70055.1"
                     /translation="MKRPIQFLRNPEPARIPADFLAFLRKLGGPTLIVLDGEERERTR
                     AMATLLHGNEPSGARAIHQWLLGGRRPRTNLLCLVASVHTALHEEAFRHRFMPGQRDQ
                     NRCFRPPFDDAPGHIAGHFLELLQQHRPECLLDLHNTSGFGPAFGVVPHEDLAHEAIV
                     SRFCSHLIVTDLRLGSLMEISGSPCPAVTVECGGARDPVADRIAREGLERYLMDEDVM
                     GTLAGERRLDVYHHPVRLELEPGASIAYCDEPLPGMDFTVAPALEHCNFGGAGPDTPI
                     GWLGERGTAALRVRNARGEDVLAQYFTARDGRLYPSQKLRLFMVTTNPQIALSDCLLY
                     ASPEREHTRL"
     gene            complement(11341..11655)
                     /locus_tag="PLW13_06490"
     CDS             complement(11341..11655)
                     /locus_tag="PLW13_06490"
                     /inference="COORDINATES: protein motif:HMM:NF023171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HQY70056.1"
                     /translation="MTEEMLFGALLALAAWLYYDGMRVREQALRVGRDYCRNVGLQML
                     DETVSRQRIGLARDGGGQVRIRRNYGFEFTSDGERRYHGEITMLGMSVQGVQVELHRI
                     LH"
     gene            complement(11648..12625)
                     /locus_tag="PLW13_06495"
     CDS             complement(11648..12625)
                     /locus_tag="PLW13_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70057.1"
                     /translation="MSTANTFGATTTTDDVLAGTDLRGTRVLVTGASGGIGLETARAL
                     AAHGAAVTLAARDAAKLERAARDIAARTGNADVDWLQIDLASFASVREAVRTYAGRHD
                     RLGVLVNNAGVMASPLMRSADGHEMQFAVCHLGHFLLTCLLVPLLERGAPARVVNLSS
                     GGHKLGGVDFDDPDFRARPYDKWVAYGQAKTANILFSVELTRRLSGCGITSNAVHPGA
                     IGTTDLARYLEPTDFAEMMRRTKFSDRIPFKSMEAGAATSVWAATAPELAGRGGLYLE
                     DCHVAAVSDDLDNGQDGYRSYASDPAAARRLWALSEQAVGEHFAWGGDD"
     gene            complement(12622..13128)
                     /locus_tag="PLW13_06500"
     CDS             complement(12622..13128)
                     /locus_tag="PLW13_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQY70058.1"
                     /translation="MASTLTRRLALLAIAALLAACAGLSGRIENPRVTVTSLRVLPAE
                     GVEQRVEVGLRLLNPNGFDLDAKGLVISLGINDVPLLNGATADVPRVPAYGEAEATVL
                     LSVNLMSGLRLVSRLMQHPEDALQYRLEARLDLRSPMWKRLTLMEQGEISPRPPASVH
                     DSNKEKTF"
     gene            13307..14434
                     /locus_tag="PLW13_06505"
     CDS             13307..14434
                     /locus_tag="PLW13_06505"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQY70059.1"
                     /translation="MRTSLCDRLGIDLPVFAFTHCRDVVVAVSKAGGLGVLGAVGFTP
                     EQLREELDWIDAHIGDKPYAVDIVIPQKYEGMGSVDPAELERQLEAMVPPEHHEFAAK
                     LLRDHGVPEWPDKDDRMGLLGWTEATATPLLEEALTRPKVKLIANALGTPPKEIIDRI
                     HASGRLVAALCGKVKQAKQHKANGVDIIIAQGSEGGGHTGEVGSIVLWPQIIDAVAPT
                     PVLAAGGIGNGRQVAAALAMGAEGVWAGSLWLSVAEAAAEPEEKECYFAATSEDTVRS
                     PSWTGKPCRMLRNDWTDAWAQPENPKPLGMPLQGLVTADAIRRTHRYVAQGDTRKVAF
                     HPIGQVVGQIRHVETARGVVQRLMEEYVDALERLNSLTGGD"
     gene            complement(14409..15557)
                     /locus_tag="PLW13_06510"
     CDS             complement(14409..15557)
                     /locus_tag="PLW13_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY70060.1"
                     /translation="MSTALILSGGGARAAYQVGVLRGVMEIHGREQLPFDVICGTSAG
                     AINAVFMAANAHRPREGVERLYRAWRGISAGDVYDHGWAAVLGNLWRIARSPFSRDGR
                     ATPGALLDNAPLRLMLRNWLDFDAVRRNLAEGVLRTLCITAMDYGSGSSVAFFEGGST
                     APWNRLYRRGERAAIALEHVMASAAIPVLFPAEGIGGSYFGDGALRQLHPLSPALKFG
                     ATRLFVIGVSAPFAPPPGMTERRRPGIGQMAAHLLNREFIDNLGADIEQARHFNEVGR
                     ALSPPDRQRHGMQPIETLVITPSAHFNEIAAQYLHKQPRTIRLLLRLLGTTSRGAGAS
                     FASYLMFDSGFCARLMDMGYRDVMAERGRVQEFLDAPAQELSRLPSAS"
     gene            complement(15554..16024)
                     /locus_tag="PLW13_06515"
     CDS             complement(15554..16024)
                     /locus_tag="PLW13_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQY70061.1"
                     /translation="MAIWLLKTEPAECSLDDIRAAAGGVMRWDGIRNYQARNLLRDAV
                     RVGDLCLVYHSGIREPALVGSAEVLRAAYPDPAQFDAASPLHDPAAKAASPRWFCIDI
                     RYREHFPRPLTLGAIKATPALAAMVLLRQGRLSVQPVTDAEWRVIAALAGLAPA"
     gene            complement(16254..17462)
                     /locus_tag="PLW13_06520"
     CDS             complement(16254..17462)
                     /locus_tag="PLW13_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70062.1"
                     /translation="MTAPDYRLIDCDNHYYEADDCFTRHIEPRFRERTVWVDRSRADG
                     IGVMMLGSKRLQFFSAAVGDHVAPPGAMVEFLRGDTEEGGAVNLNPVAAQDFPQFIER
                     GARLVEMDRQGVEAAVQIPTLGVGVEWQMRADPDFHEVLYPSIRSFNRWLAADWGWGG
                     DGRIFCTALMSLADLPHALEELERLIAEGVRLVLLTTGPVNGRSPADPYFDPFWARVQ
                     EAGLVVVFHIGATGFNAMQAAPWGEPANPPSHRFTPFNTFVGMGERTIIDQIAALVFH
                     NLPDRFPGLRMLIVEYGAAWLPHLLKTLDKIYRLGDHRSRWAFGKPSLKPSETFQRQF
                     RIVPFFEDDIAAVARAAGVQCVLHGSDYPHPEGLHASADMLRELGSFSAAEQRRIMRG
                     NAAELLGLAP"
BASE COUNT         2770 a         6237 c         6087 g         2554 t
ORIGIN      
        1 ggcgcagcag gccgtggtcg acgaacacgc aggtgagctg gtcgccgatg gcgcgcgcga
       61 gcagtgcggc gaccaccgag gaatcgacgc cgccggagag cccgagcagc accttgtcct
      121 tgcccacggt cttgcggatc gaggcgatcg cgtcgtcgat gatgctgccc ggcgtccaca
      181 gcccctcgca gccgcagatg ccgcgcacga aatgctcgag gatgcgcccg ccctggtgcg
      241 tgtgcgtgac ctcggggtgg aactggatgc cgtagaaacc acgcgactcg tcgcacatcg
      301 ccgcgaccgg cgcgctgtcg gtggccgcga gcagctcgaa gcccggcggc agcgccacga
      361 ccttgtcgcc gtggctcatc cacacgtcca ggcgcgccgc gccttcctcg tcgacgtggt
      421 cgctgatgcc gtcgagcagg cggctggagg ccgtgacgcg cacctgcgcg tagccgaact
      481 cgcgccggtt cgagggctcg acgcgcccgc cgagctgtgc ggccatcgcc tgcatgccgt
      541 agcagatgcc gagcagcgga atgcccatgt tgaacagcgc cgcgggcacg cgcggggtgc
      601 tcttgccggt gaccgattcc gggccgccgg agaggatcac gccgctgggg cggaacgcct
      661 tgatcgcggc ggcgggggtg cgccagctgt agatctcgca gtagacgccg agctcgcgca
      721 cacgccgcgc gatgagctgc gtgtactgcg agccgaagtc gagaatcagg atgcgctggg
      781 cgtggatatc ggtgttcatc gcggtcattt tctccgtgcc ggaaacaagc gcggggcctg
      841 ctcggccccg cgggaaatca gttgcggtcg cgggcatggc ccgctcctac atctggcatc
      901 gtcgcaaccc ctgtaggagc ggcccatggc cgcgacatcg cgcgcatggc gcgctcctgc
      961 aaattcgggc ctgtcagcgc accgggtagt tgggcgcttc cttggtgatg ctgacgtcgt
     1021 gcacgtggct ctcgttgacg cccgccgagg tgatgcgcac gaagcgcggc cgcgtgcgca
     1081 tggtctcgat gtcggggctg ccggtgtagc ccatcgccgc gcgcaggccg cccatcaact
     1141 ggtgcacgat ggccgagatc ggacccttgt agggcacgcg tccctcgatg ccttcgggca
     1201 ccagcttctc gacgccctcg gcggcgttct ggaaatagcg gtcgctggaa cccttggcca
     1261 tcgcgcccag cgagcccatg ccgcggtagg ccttgtagct gcggccctgg tagagctcga
     1321 tctcgcccgg cgcctcctcg gtgccggcga gcatgctgcc gaccatgatc acgtgcgcgc
     1381 cggccgccac cgccttggcg aggtcgcccg agaagcggat gccgccgtcg gcgatcagcg
     1441 gcacgccggt gccctcgagc accgccgcca cgttggcgac cgcggtgatc tgcggcaccc
     1501 cgataccggt cacgatgcgc gtggtgcaga tcgaaccggg accgatgccc accttcacgc
     1561 cgtcgacgcc ggcatcgagc aggtatttcg cggcttccgc cgtggcgatg ttgcccgcga
     1621 tcacctgcac ctgcggatac cggctcttga tgctgcgcac ggtgtcgcgc acgttgcgcg
     1681 aatgcccgtg cgcggtatcc accaccagca cgtcgacgcc cgcctcgatc aggcgcacca
     1741 cgcgctcgtc ggcggcgccg ccgcccacac cgaccgccgc gccgacacgc aggcgcccgt
     1801 cggcgtcctt gcaggcgttg ggatagcgct cggccttgaa gatgtccttg gcggtgatca
     1861 tgccgcgcag gcgaaacgcg tcatcgacca ccagcacctt ctcgatgcgg tggcggtgca
     1921 gcagtttctt cacgtcggcg aggtcggtac cctcgagcac cgtcaccagt tcgcgcttgc
     1981 gcgtcatgat cgaggagacc ggctcgtcga ggttggtcgc gaagcgcacg tcgcggctgg
     2041 tgacgattcc gaccagctcg cgcttgcggt tgagcaccgg cacgccggaa aaattgcgct
     2101 cgatggtcag gcggatcagg tcgcggatgg aacgcgagga gtcgatggtg accggctctc
     2161 gcaccacgcc gctctcgaaa cgcttcacgg cgcggacctc ttcggcctgc tgctcgggcg
     2221 tcatgttctt gtgcacgatg ccgatgccgc cttcctgggc aatcgcgatc gcgagcttgg
     2281 actcggtgac cgtgtccatg gccgccgaca tcaggggaat gttgagggtg atatcgcggg
     2341 tcaggcgtgt ctgtagcttg acgtccttcg ccacgacttc cgattcgccg ggcaccagca
     2401 gcacgtcgtc aaaggtgagc gcttcttcct ggacccgcag catggcaact ccgcaagggc
     2461 ttgtttactg aaggcgcggc attatacggg ccgcaccccg cacggtaaac cgcccgtccc
     2521 gtgggtccgg attcatccgg acaagctccc cctgtgggtc cggattcatc cggacgaatg
     2581 tccggttaaa accggaccca catcaggccc acatcaggcc ccagatgcag cccccaccgg
     2641 gcaggccacc cccgtgccac cgatgccgca gtaacccccg gggttcttgg ccaggtactg
     2701 ctggtggtat tcctcggcgt agtagaacgg ccccgcggcg cggatctccg tggtgaccgg
     2761 gccgaatccc gcggcgcgca acacctgctg gtacgcatcg cgcgaggcca gcgcgaggcg
     2821 cagctgctcc ccggaatgcg tgtagatacc cgagcggtac tgggtgccga tgtcgttgcc
     2881 ctggcgcatg ccctgggtgg ggtcgtggct ctcccagaac acgcgcagca gctcggggta
     2941 gctcgtgcgc gccggatcga agaccaccag caccgcctcg gtgtgcccgg tgcgcccgga
     3001 gcacacctcg cggtagctgg ggttcggcgt gagccccgcg caatagccca ccgcggtcga
     3061 gtagacgccg ggctgctgcc agaagcgacg ctcggcgccc cagaagcagc cgagcgcgaa
     3121 ctcggccagt tccagtccgg gcgggaacgg cggcacgatg cgcgcgccgt tcacgaagtg
     3181 cagcggcggc acggtcatgg gctcggcgcg cccgggcaac gggtcggcgg gcatcgggat
     3241 tgccggggtt gtgctcatgg tgcggattcc ggatcggcgt ctggacgacg catggatgtc
     3301 aatcgagtac gctggcaccg gccgaagcga tcatccgcgc cgcctcgcca aagcgcagcg
     3361 cctcggtgct ggaggcgttg ccgtcgatgc cgcgcttgta cacgccctgg cagatcgccg
     3421 cgaggcgaaa gaagctgatc gcgagataga aggtccagtc cggtatcgcc ccgataccca
     3481 tcgcggcgca gtagcgcgcg acgtactcct cctccctcgg gatgccgagc gccgcgcggt
     3541 ccacgccgcc cagcccgcgc agcgcggccg cgccatgagg caggcgccac tgcatgcact
     3601 ggaaggcgag atcggcgaag gggtggccca gcgtcgagag ctcccagtcg agcaccgcga
     3661 cgatgcggtc ttcggtggcg tcgaacatca ggttgtcgag gcggtagtcg ccgtgcacca
     3721 gcaccacgcg cccgtcgtcc gccggcaggc gcccggcgag ccacgtcatc agcgcctcca
     3781 tggcctcgat gcgctcggtc tcggcggcgc ggtactgctt cgtccagcgg ttgagctggc
     3841 gcgcgtagta gttgcccggc tggccgtagt ctgcgaggcc caccgccccc acatcgacgc
     3901 tgtgcagcgc taccagcacg cggttcatct cgtcgtagac gcgcgcgcgt cgcgcggcat
     3961 cgagctcggg cagcgtgggc tcccacagga tgcgcccgtc gaggaactcc atcacgtaga
     4021 acatgctgcc gatcacggtg tcgtcgtcgc acagcgcgag cgcacgcggc accggcacat
     4081 cggtgggtgc cagcgcgcgc agcacgcgat actcgcggtc caccgcgtgc gccgaggcca
     4141 gcagcgcgcc ggggggcttg cgccgcagca cgtagcgtcc gctgtcggcc tcgagcagga
     4201 aggtcgggtt cgactgcccg ccggcgaact tgcgcgccgt gtgcaggccg tgaaaatccc
     4261 ccaccgcctg ttccagccac gcgcccagtt tctccacggg caaatccgtc ggtgccgcct
     4321 tgctcatgtc atcagaatcc ttgtagcgct gcccgggttc agccataggt tatcgcatcc
     4381 tcgcgcccgg cgcgctcgag atgcggcacg gcgttccagc tcgacagcag gaagcgctcg
     4441 cggttgaggt gaaaatgcga caccgaggtg ttacgcacct gcatgttcag gtcggcgatg
     4501 tgcgcgggcg agagcccgag ccggcccccc agcagcgcgg acatcgcgcc gccggaactg
     4561 atcgcgagca ggcgcgcgcc ccgcgccgtc agcgcctgca gcgcggcggc ggcgtccgta
     4621 gtgcgccgca tgaaatcgcc ccaggtctcg ggcagggggc ccggcagctc cccggcggcc
     4681 caggccagca tcgccagttt cagcagccgg tagaacgcgg gcttcgtggc cggctcggca
     4741 cgccaggccg ccacgcgggc atcgtcgggg tgcgcgacgg cgaaggccgc gaccagggcg
     4801 cggaaatcgt actcgtcgag cccgggcagc tcgatcgcat caccgcccgt atcgagttcc
     4861 atgccctcga gtattcccgc cagcgtctgg cgatggcggc gcagggtgcc cgtgaccagc
     4921 acgtcgaagc ggatgtcgcg cgaggcgaag tattcgccga gccagcgcga ctggcgctcg
     4981 cccagcgcgg agaggcaatc gtaatcggca ctgtgaaacg aggcctgggc gtgcctgacg
     5041 agatagagtt cggccactgg cggtttcccg gaaaacttcg aaagttcgct gccggggcat
     5101 ggtaatggcg ccggcgccgc gcggtccatc catgtgctca gacgcggcaa gcgacgctgt
     5161 tttctagtat gagctttcta atcccgagga ctactctggc gccatcgtga acacaagggt
     5221 tcacggctaa tccccgattc cagccacagg agaatcacca tggctcaaga cgcagcgaac
     5281 atcatcgaag aaaccgtgaa gccggcggcc cgtcgcgtcg tgcgccgtgc caaggcggcg
     5341 gcaacgagca agcccgcggc acgcaagagc gccgctacga aggccgcggc accgaagcgc
     5401 gcctacaagc gcaaggccac tgccgcgagc aaggcgaagc cggcaatctc gctgaagaag
     5461 gcctccgaga ccgcgaagga agttgcctac gtgcaactcg gcatctgcgg caaggtctac
     5521 gacgaggtga acacccgcgt gaccaaggcg cgcaaggtgg ctccgaagaa atggagcgac
     5581 ctggtcaagc gcggcgagcg cgtgcaacag gacctcgaca aggtgcagaa ggacctgacc
     5641 aaggacctgc gcaagcgcgt caacaagctc gagattccgg cgccgatcga aacccgcgtc
     5701 acgaagttca ccaaggcggt caagaagttg acggctcgcg tcaacaaggc agcctgatcc
     5761 ccgttcacgg tctcacaacc cgctcaaagc caccccccgg ggtggctttt ttttgcgccg
     5821 cgaatcacca tcggcccgcc acatccacga cgcggacatc cggggcaggc atgcggggca
     5881 atctggacat cgccgtgatc ggcggcggca tcaatggcat cagcagcgcg accgagctcg
     5941 cggccgaggg gcaccgcgtc gaactgttcg agcgcggcga actgatgggc gagaccagca
     6001 gccgctcctc gaagctgctg cacggcggcc tgcgctacct cgagaccggc gagctgcgcc
     6061 tggtgcgcga ggcgctgggc gaacgctcgc gctggctcgc ggactgcccg cagcacgcac
     6121 agccgctgcg cctcgcgctg ccggtgtatg ccggcgtgtc gcgctcaccg ctgctgctcg
     6181 gcgccggcct cgcgctctac ggcctgctgg ccggcaggca gggtctcggt cgatcgcagt
     6241 ggctgggcgc gcgcgagttc gcgcgcctga atccgcaact gcagcccgac gggctgcgcg
     6301 ggggcttcta cttcttcgac ggccgcatgg acgatcgcgc gctcgggtta tggctggccg
     6361 gcgtggcgcg ctcgcgcggc gtggtgatcc gcgagcacgc gccggtgctg gggatcgatg
     6421 cgggtggcgg cgtgcggctc gcaaccggcg agcagcgctt cgacctggtg gtgaacgcgg
     6481 ccggcccctg ggccgaggtg ctgttgcgcg actccgggct gcaaccggcc cacggcatcg
     6541 actgggtgcg cggctcgcat atcgtgttcg acgaaccgct cgcgcaggcc tgcctgctgc
     6601 aggtcccggg cgagaagcgc atcttcttcg tgctgccctg gcaggggcgc accctggtgg
     6661 gcaccaccga ggtggcacag tcgcgcgagg agccgatcgc ggcgagcgag gcggaaatcg
     6721 actacctgct cgcggcccgc aaccactatt tccgcgcggc gaagacgcgc gccgacgtcg
     6781 ccgaggtgtt cgccggcgtg cgcccgttgc tgcatttcga gggtgacccg ggtcgcgcca
     6841 gccgcgagta cgcgctggcg cgccaggacc gcgtgctcag catctacggc ggcaagtgga
     6901 ccacggcgcg cgcgctcggg cggcaggtcg cgcggatggt cagggaatca ccaccgccgc
     6961 cccggtgaag cgcccggcgc gcagatcgtc gagcgcccgg ttcacgtcgg cgagcgcgta
     7021 gcgatgcacc tcggtgcgca ccggcagcgc ggccgccagc ggcaggaact cctcgccgtc
     7081 gcggcgcgtg aggttcgcga ccgactcgat gtggcgctcg ccccacagat cggcatacgc
     7141 gaatgacgga atgtcgctca tgtggatccc ggcacagatc acgcgcccgc ccttgcgcac
     7201 cgcgcgcagc gcccgcggca ccagctcgcc ctcgggcgcg aagatgatgg ccgcatcgag
     7261 cgcgaccggc gccggcgcgc tgctgtcgcc cgcccacacc gcgccgaggc ggcgcgcgaa
     7321 atcctgcgcc tcttgatcgc ccgggcgcgt gaacgcatag acctcctgcc cgtggtggcg
     7381 cgccacctgc accaggatgt gggccgcggc cccgaagccg tagaagccga tgcgccgcgc
     7441 cgcgcgcgcg ggacgataac tgcgataacc gatcaggccg gcgcacagca acggggcggc
     7501 ctggacggca tcgaagccgg gcgggatggc aaagcagtag cgcgcgtcgg ccacgcagta
     7561 ctgcgcgaag ccgccatcga cctggtagcc ggtatagacc gcgccgtcgc agaggttctc
     7621 ctcgccggcg cggcagaagt cgcagtcgcc gcagctgccg ccgagccacg gcacgccgat
     7681 gcgctcgccg acggcgaagc ccttgacgcg atcgccgcgg gccacgacct cgccgacgat
     7741 ctggtgcccc ggcaccagcg gcaggcgcgg ctcgcgcagc tcgccatcca ccacgtgcag
     7801 atcggtgcgg cagatgccgc aggccagcac ccgcagcagg acgccctcgg gccccggtgc
     7861 cggcacggtg cgcatctcgg cgcgcagcgg ctcgccgggg gcgtgcagca ccatcgcgcg
     7921 catcgtcgag ggcatcgtca tgaagctgta actccccgcg cggacgctga aacctgcagc
     7981 attggcagga tcgtgtcgtc gcagctgttg atcgggcgac cgtgatgggt ttactgtaat
     8041 tcgcacccgg cgttgacacc agcgcaacgc cccagcagga ggagacggct tttccctacc
     8101 gtgaaccctc gaactgcgca gcggggaggc acatgctgaa cccccggatc ctgcgcctca
     8161 atgtcgccgg ccagcccgtc gaatggctga gctggcagga agcaacctgt ctctacgcgc
     8221 gcgacatggt ggcgtggacg ctgggcgacc aggtgcgcac cgttttcggc ggcatgtcgc
     8281 gcctcaccgg cctgcaatca tcgatcgcgc tgcacagcat catcgcctgc gccggccacg
     8341 tgtcgtgcaa gccgcgcctg gtgccgccgc tgaccaaccg cgcgctgttc cgccgcgacc
     8401 tgcacctgtg catgtactgc ggcgtgcgtt tcggcgaacg cgaactgacg cgcgaccacg
     8461 tgctgccgaa atcgcgcggc ggccgcgacc actggcgcaa cgtggtggcg gcctgcaagc
     8521 gctgcaacca gcgcaagggc aatcgcctgc ccgaggaagc cggcatggag atgctcgcgg
     8581 tgccctacca gcccaacacg gccgagtggc tggcgctgat caacagcgac cgtatccgcg
     8641 gcgaccagat ggaattcctg cgcacgcagt tctcgccaca ctcgcgctgg agctgaagct
     8701 tggggatagc gatcgaccgc acggatttct cggcgcgcga tcacgcgaac ttcgcccgca
     8761 agctgcgcca gaacctcgac gcactgaaga gcctgctggc gcagcccggc ttcggggcgg
     8821 ggccctcgag cataggcgcc gaggtcgagc tcttcatcat cgacgcccgc gggcgcccta
     8881 agccggtgaa cctcgagatc ctcgcgcgca gcggcgatcc acggctcacg ctggagctga
     8941 accgcttcaa cctggagtac aacctgacgc cggtcgcggc caccggacgc cccttttcgc
     9001 gcatcgaggc gcagatcggc gatgccctgg cgcgcgtgaa cgcggccgcg gcccccgcgc
     9061 aggcacgcgt gatcccgatc ggcatcctgc caacgttgcg cgcacgcgat ttcggcccgc
     9121 acgcgatgac cccgcagctg cgctaccagg cgctggcggg tgcgctggcg cgctcgcgtg
     9181 gcgagcggtt cgcgatccgc atcggcggcc cggaaccgct gcgactggtg gtcgaggacg
     9241 tgacgctcga gggcgccaac acctcgctgc agatccacta ccgcgtgcca ccgcgggaat
     9301 tcgcgcgcag cttcaacgcg atccagctgg tgacgcccgt ggtgctggca agcgcctgca
     9361 actcgccgct gctgctcggg cggcgcacct ggcaggagac gcgcgtgccg ctgttcaagc
     9421 tcgcggtcga cgggcgcgac cgcgacagcc gcgccctgca cctgccgccg cgggtgcatt
     9481 tcggcaccgg ctgggtgcgc gagggcgcgt acgaactgtt ctccgccgcg gtgcacctgc
     9541 acgagccgct gctgccgatc tgctcgcgcg agaatgcgct ctcgcgcctg cgcgcgcagc
     9601 gcatccccgc gctggacgaa ctgcgcctgc accacggcac gctgtggccc tggaaccgcc
     9661 cggtctacga tcccgcgcac ggcggtcatc tgcgcatcga gctgcgcgcg ttgccggcgg
     9721 gccccagcgc tgccgacatg atggccaatg ccgcgttcgc gctcggcctg gcgcgcgggc
     9781 tgcgcgatcg catcgacgac tggctgccgg gcatcccctt cgccatcgcc gagagcaatt
     9841 tctaccgcgc cgcggagcat ggcctcgatg ccacgctgct gtggccggac gcacacggca
     9901 cgctggcgcg gcgcaacgcg accgatatcg cgcgcgaact gctgcccgtc gccgcgcgcg
     9961 ggctgcgcct gctcggggtg cacgacgcgg aatcggcgca ttacctgggc atcatgcgca
    10021 cgcggctcga acggcgcacg aacggtgcca cctggcagct cgcccagctc gccaggctgg
    10081 aagcgcggca cgggcgatcg cgcgccctgt cgctgctcgt cgggcactac gcccgccacg
    10141 cactcggtaa cactcccgtg gccgaatggc cggacatcga atgaagcgac cgatacagtt
    10201 cctgcgcaat ccggagccgg cacggatccc ggccgatttc ctcgccttcc tgcgcaagct
    10261 cggcggtccc acgctgatcg tgctcgacgg cgaggagcgc gagcgcacgc gggcgatggc
    10321 aaccctgctg cacggcaacg agccctcggg cgcgcgcgcg atccaccagt ggctgctcgg
    10381 cgggcggcgc ccgcgcacca acctgctctg cctggtggcc tcggtgcaca cggcgctgca
    10441 cgaggaagcg ttccgccacc gcttcatgcc ggggcagcgc gaccagaacc gctgctttcg
    10501 tccgcccttc gacgatgcgc ccggccacat cgccggacac ttcctggaat tgctgcaaca
    10561 gcaccgcccg gaatgcctgc tggacctgca caacacctcc ggcttcggtc cggccttcgg
    10621 cgtggtgccg cacgaggacc tggcgcacga ggccatcgtc tcgcgcttct gcagccattt
    10681 gatcgtcacc gacctgaggc tcggctccct gatggagatc agcggctcgc cctgccccgc
    10741 cgtcacggtg gaatgcggcg gcgcgcgcga cccggtggcg gaccgcatcg cgcgcgaggg
    10801 gctggagcgc tacctgatgg acgaggacgt gatgggcacg ctggccggcg agcgccgcct
    10861 cgatgtctac caccacccgg tgcggctgga gctggagccc ggcgccagca tcgcctactg
    10921 cgacgagccg ctgccgggca tggacttcac cgtggcgccg gcgctggagc actgcaactt
    10981 cggcggcgcc ggccccgata cgcccatcgg ctggctgggg gagcgcggca ccgcggcgct
    11041 gcgcgtgcgc aacgcccgcg gcgaggacgt gctggcgcag tacttcacgg cccgcgacgg
    11101 gcggctctac ccgtcgcaga agctgcggct gttcatggtg accaccaacc cgcagatcgc
    11161 gctgagcgac tgcctgctct acgcctcgcc cgagcgcgag cacacccgcc tgtaggttcg
    11221 gcttcagagg gtgtcacaaa acgctttggc aaccggcgac aacgcgacga tcagggcgta
    11281 ggagcggccc atggccgcga tatcgcgcgc atggcgcgct cctgcaacgg acccacgaca
    11341 tcagtgcagg atgcggtgca gctccacctg cacgccctgc acgctcatgc cgagcatcgt
    11401 gatctcgccg tggtagcggc gctcgccgtc gctggtgaat tcgaagccgt agttgcgccg
    11461 gatacgcacc tgcccgccgc cgtcgcgcgc cagccctatg cgctggcggc tcacggtttc
    11521 atcgagcatc tgcagcccca cgttgcggca gtagtcacgg cccacgcgca gcgcctgctc
    11581 gcgcacgcgc atgccgtcgt agtacagcca cgccgccagt gccagcagcg cgccgaacaa
    11641 catctcctca gtcatcgccg ccccacgcga aatgctcgcc gaccgcctgc tccgacagcg
    11701 cccacaggcg ccgcgccgcg gccggatcgc tcgcatagct gcggtagccg tcctggccgt
    11761 tgtcaagatc gtcgctcacc gcggccacgt ggcaatcctc gagatagagc ccgccacgcc
    11821 ccgcgagctc cggggccgtg gccgcccaca ccgacgtcgc cgcacccgcc tccatcgact
    11881 tgaacggaat gcggtcgctg aacttcgtgc gccgcatcat ctcggcgaag tccgtcggct
    11941 cgaggtagcg ggcgaggtcg gtggtaccga tcgcccccgg gtgcacggca ttcgacgtga
    12001 tcccgcagcc ggacaggcgg cgcgtgagct ccaccgagaa caggatgttg gcggtcttgg
    12061 cctggccgta cgcgacccac ttgtcgtaag gacgcgcgcg gaaatccgga tcgtcgaagt
    12121 ccacgccgcc cagcttgtgg ccgccggagc tcaggttcac cacgcgcgcc ggcgcgccgc
    12181 gctcgagcag cggcaccagc aggcaggtga gcaggaaatg cccgaggtgg catacggcga
    12241 actgcatctc gtggccatcg gccgaacgca tcagcgggct cgccatcacg ccggcattgt
    12301 tcaccagcac gcccaggcga tcgtgccggc ccgcataggt gcgcaccgcc tcgcgcacgc
    12361 tcgcgaacga ggcgagatcg atctgcagcc agtcgacgtc ggcattgccg gtgcgcgcgg
    12421 cgatgtcgcg cgcggcgcgc tcgagctttg ccgcgtcgcg cgccgcgagc gtcaccgccg
    12481 caccgtgcgc ggccagcgcg cgcgccgtct cgagcccgat gccgcccgag gcgccggtga
    12541 ccagcacgcg ggtgccgcgc agatccgtgc cggccagcac gtcgtcggtg gtggtcgtgg
    12601 cgccgaaggt gttcgcagtg ctcaaaatgt cttctccttg ttgctgtcgt ggaccgatgc
    12661 cggcgggcgc ggcgagatct cgccctgctc catcagcgtg aggcgcttcc acatcgggct
    12721 gcgcagatcg aggcgcgcct ccaggcggta ctgcagcgca tcttccgggt gctgcatcag
    12781 gcggctcacc aggcgcagcc cgctcatcag gttgaccgac agcagcaccg tcgcctcggc
    12841 ctcgccatag gccggtacgc gcggcacgtc ggcggtcgcg ccgttgagca gcggcacgtc
    12901 gttgatcccc aggctgatca ccaggccctt ggcgtcgaga tcgaaaccgt tgggattcag
    12961 caggcgcagc cccacctcga cgcgctgctc cacgccctcg gccggcagca cccgcagcga
    13021 ggtgacggtg acgcgcgggt tctcgatgcg cccggaaagg ccggcgcacg ccgccagcag
    13081 tgcggcaatg gcgagcaggg ccagccgccg ggtcaacgtc gatgccatgg cacgaatcct
    13141 tcgggttatg gggcgagcag tgtaggggct gcccccgccg agcggcaacc gccggggacg
    13201 gcgcctgatc gctttggaca cggaccgttg acccgcattt gcattgcctg cgcctcgctg
    13261 caggagccat gattggcgcc ttcgcaataa ccagtggtaa gccgccatgc gcacttcgct
    13321 ctgtgaccgc ctcgggatcg acctccccgt cttcgccttc acccactgcc gcgacgtcgt
    13381 cgtggccgtc agcaaggccg gcggcctcgg cgtgctgggc gccgtgggct tcacgccgga
    13441 gcagttgcgc gaggagctcg actggatcga cgcgcacatc ggcgacaagc cgtacgcggt
    13501 cgacatcgtg atcccgcaga aatacgaggg catgggcagc gtggatcccg cggagctgga
    13561 acgacagctc gaggccatgg tgccgccgga acaccacgag ttcgccgcga aactgctgcg
    13621 cgatcacggc gtgcccgagt ggcccgacaa ggacgacagg atgggattgc tgggctggac
    13681 cgaggccacc gcgaccccgc tgctggagga ggcgctcacg cgcccgaagg tcaagctgat
    13741 cgccaacgcg ctgggcacgc cgccaaagga gatcatcgac cgcatccatg ccagcggacg
    13801 actggtcgcc gcgctgtgcg gcaaggtcaa gcaggcgaag cagcacaagg ccaacggtgt
    13861 ggacatcatc atcgcgcagg gctccgaggg cggcggccac accggcgagg tgggctcgat
    13921 cgtgctgtgg ccgcagatca tcgacgcggt ggcgcccacg ccggtgctcg cggcgggcgg
    13981 gatcggcaac ggccggcagg tggccgcggc actcgcgatg ggcgccgagg gcgtgtgggc
    14041 cggttcgctg tggctgagcg tcgcggaagc cgccgccgaa cccgaggaga aggaatgcta
    14101 tttcgccgcc accagcgagg acaccgtgcg ctcgccgtcg tggaccggca agccctgccg
    14161 catgctgcgc aacgactgga ccgacgcctg ggcgcaaccg gaaaacccca agccgctcgg
    14221 catgccgctg cagggcctgg tcaccgccga cgcgatccgc cgcacccatc gctacgtcgc
    14281 gcagggcgac acgcgcaagg tggcgttcca ccccatcggc caggtagtcg ggcagatcag
    14341 gcacgtcgag accgcgcgcg gcgtggtgca gcggctgatg gaggaatacg tggatgcgct
    14401 cgagcgcctc aactcgctga cgggaggcga ctgagctcct gcgccggcgc gtccaggaat
    14461 tcctgcacgc gcccgcgctc ggccatcacg tcgcggtagc ccatgtccat caggcgcgcg
    14521 cagaagccgc tgtcgaacat caggtaactg gcgaaactcg cgccggcgcc gcgagacgtg
    14581 gtccccagca ggcgcagcag cagccggatc gtgcgcggct gcttgtgcag gtactgcgcg
    14641 gcgatctcgt tgaagtgcgc cgacggtgtg atcaccagcg tctcgatggg ctgcatgccg
    14701 tggcgctggc gatcgggcgg cgagagcgcc ctgcccacct cgttgaaatg ccgcgcctgc
    14761 tcgatgtccg cgccgaggtt gtcgatgaac tctcggttca gcaggtgcgc ggccatctgc
    14821 ccgatgccgg ggcggcgacg ctcggtcatg ccgggcggcg gcgcgaacgg cgccgacacg
    14881 ccgatcacga acagccgtgt cgcgccgaac ttcagcgccg ggctgagcgg atgcagctgg
    14941 cgcagcgcgc cgtcaccgaa atagctcccg ccgatgccct cggccgggaa cagcaccggg
    15001 atcgccgccg aggccatcac gtgctccagc gcgatcgcgg cgcgctcgcc gcggcggtag
    15061 agccggttcc acggcgcggt ggagccgccc tcgaagaagg ccaccgagct gccgctgccg
    15121 taatccatcg cggtgatgca gagcgtgcgc agcacgccct cggcgaggtt gcgccggacg
    15181 gcgtcgaaat cgagccagtt gcgcagcatc agccgcagcg gggcattgtc gagcaacgcg
    15241 ccgggcgtgg cgcgtccgtc gcggctgaac ggcgatcgcg cgatgcgcca caggttgccg
    15301 agcacggccg cccagccatg gtcgtagaca tcgccggcgc tgatgccccg ccaggcgcgg
    15361 tacaggcgct cgacgccctc gcgcggacga tgcgcgttgg cggccatgaa caccgcgttg
    15421 atcgccccgg ccgaggtgcc gcagatcacg tcgaagggca gctgctcgcg gccgtggatt
    15481 tccatcacgc cgcgcagcac gccgacctgg taggccgcgc gcgcgccgcc accggagagg
    15541 atcagcgcgg tgctcacgcc ggcgccaatc cggccagcgc cgcgatcacg cgccactcgg
    15601 cgtcggtcac gggctgcacc gacagccgcc cctggcgcag cagcaccatc gcggccagcg
    15661 ccggcgtcgc cttgatggcg cccagcgtga gcgggcgcgg gaagtgctcg cggtagcgga
    15721 tatcgatgca gaaccagcgc gggcttgcag ccttcgccgc cggatcgtgc agcgggctcg
    15781 cggcgtcgaa ctgcgccgga tccggatacg ccgcgcgcag cacctcggcg ctgccgacca
    15841 gcgccggctc gcggatgccc gaatggtaga cgaggcacag gtcgcccacg cgcaccgcgt
    15901 cgcgcagcag gttgcgtgcc tggtaattgc ggatgccgtc ccagcgcatc acgccgccgg
    15961 cggcggcgcg gatgtcatcg aggctgcatt cggccggctc ggtcttcagc agccaaatgg
    16021 ccatggccag ttcctcccgc ctccgtaggt cgggcttcag cccgacaagg gtacggcgca
    16081 gccgaacccg ccgccttgcc gtatccaccg cgactgtcgg catgaatgcc gacctgcatg
    16141 caggtcgggc ttcagcccga cattggccgg gatcctggca cctgttcgac tgaagtcgaa
    16201 cctacgacag tctagtccag cgatgccgcg cgcgccgcgc aggggcaagc gcctcagggt
    16261 gccagtccca gcagctcggc ggcattgccg cgcatgatgc gccgctgctc ggcggcgctg
    16321 aaggaaccga gctcgcgcag catgtcggcg gaggcatgca ggccctctgg gtgcgggtaa
    16381 tccgagccgt ggagcacgca ttgcacgccg gccgcgcgcg ccacggcggc gatgtcgtcc
    16441 tcgaagaagg gcacgatgcg gaactgccgc tggaaggttt cgctcggctt cagcgacggc
    16501 ttgccgaagg cccagcgcga acggtggtcg ccgagccggt agatcttgtc gagcgtcttc
    16561 agcaggtgcg gcagccaggc cgcaccgtac tccacgatca acatgcgcaa ccccgggaag
    16621 cggtccggga ggttgtggaa gaccagcgcc gcgatctggt cgatgatggt gcgctcaccc
    16681 atgccgacga aggtgttgaa cggtgtgaag cggtgcgagg gcgggttcgc gggctcgccc
    16741 cagggcgcgg cctgcatcgc gttgaaaccg gtggcaccga tgtggaacac caccaccagc
    16801 ccggcctcct gcacgcgcgc ccagaacggg tcgaaatacg gatccgccgg cgagcggccg
    16861 ttgaccggtc ccgtggtgag cagcaccagg cgcacgccct cggcgatcag gcgctcgagc
    16921 tcctcgagcg cgtgcggcag gtccgcgagc gacatcagcg cggtgcagaa gatgcgcccg
    16981 tcgccgcccc agccccagtc cgcggccagc cagcggttga agctgcggat gctcgggtag
    17041 agcacctcgt ggaagtcggg atcggcgcgc atctgccact cgacaccgac gccgagcgtg
    17101 ggaatctgca ccgcggcctc gacgccctgg cggtccatct cgaccagccg cgcgccgcgc
    17161 tcgatgaact gcgggaaatc ctgcgccgcg accgggttca ggttcaccgc gccgccctcc
    17221 tcggtgtcgc cgcgcaggaa ttcgaccatc gcgccgggag gtgcgacgtg gtcgcccacc
    17281 gcggcgctga agaattgcag gcgcttggag ccgagcatca tcacgccgat gccgtcggcg
    17341 cggctgcgat ccacccacac cgtgcgctcg cgaaagcgcg gctcgatgtg gcgcgtgaag
    17401 cagtcgtcgg cctcgtagta gtggttgtcg cagtcgatca ggcgatagtc gggcgcggtc
    17461 atggcagtgg ctctcgtagg ttcgcattca tgcggacatg atgtgggtcc gcattcatgc
    17521 ggacggttgt ccggtttaaa ccggacctgc gtcgggagat cgcgccattc tacgcaacgc
    17581 ctccgccacc ccgcgattac aaagccttca ggcgggaaag aaacgcgaga tcgtgtcgac
    17641 cacgcagg
//
LOCUS       DAORUQ010000052        17442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2928_length_17442_cov_6.770691, whole genome shotgun sequence.
ACCESSION   DAORUQ010000052 DAORUQ010000000
VERSION     DAORUQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17442
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_17442_cov_6.770691"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1991)
                     /locus_tag="PLW13_06525"
     CDS             complement(54..1991)
                     /locus_tag="PLW13_06525"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY70063.1"
                     /translation="MTHRINPAGEPITDDDATIAAALEQASVPCLMMSMIHMTGDASL
                     LDGDIRPVLAFLNDMQCAMPAVQQAELRRRALEVIKAYRDRGCTLPPAPDTGTLQRMM
                     SFLVAGEVPAAYVPMMLEEMELDGRDAREVSLAHIPQQAREALPVLIIGAGMSGILAG
                     IRLQEEGVPYVIIEKNHGVGGTWYENTYPGCRVDVGNHLYCYSFEPNLDWQEFFARQP
                     ELRRYFENTARKYGVEPHIRFDTEVQAADFDEASGEWRVRVRAKDGATQTLRARVLIS
                     GVGQLNRPKLPDIEGLERFRGPAFHSACWDHSVSLAGKRVAVIGTGASAFQLVPEIAK
                     EAAQVYTFQRTPQWMFPNPDYHRAVSEGASWCFRHLPYYGRWYRFLLFWPATDGTWEG
                     TFVDPQWPHQERSINAINEFTREMFTDWIRQQCGEDEALFERVLPRYPPLARRTLQDN
                     GSWLQALRRDNVQLVDREVVALTETAVRTADGSEFEADVVVYATGFHANRYLWPMEIT
                     GRNGVKLGEQWGDDPAAYLGITVPDFPNLFVMYGPATNLAASGSLIFHGECQARYIMG
                     CIRAMLERDAKTLQVRREVHDAYNRRLEETLARTVWSHPAVAHSWYRNSKGRVTVLSP
                     WKLLDYWTWTREPKLDEYELA"
     gene            2318..3469
                     /locus_tag="PLW13_06530"
     CDS             2318..3469
                     /locus_tag="PLW13_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HQY70064.1"
                     /translation="MSDAIIPFKVEIGSAGIEDLRRRLAATRWPDAETTGDWSQGIPL
                     AYMQEVCTYWATQYDMQRVAKRLNAFPQFRTRIDGLGIHFLHVRSPHADALPLVMTHG
                     WPGSVIEFLNVIEALTDPTRHGGEAKDAFHVVCPALPGYGFSDKPPARGWGVERIGRA
                     WGELMQRLGYASYVAQGGDWGSMVTSSIAMTETAHCRGIHLNMPLVAPDPDTMSDLSA
                     SEQAALAAMQDYYENESGYSKQQSTRPQTLGYGLADSPAGQAAWILEKFRAWTDCGEG
                     EARHPENALTRDELLDNVMMYWLTDSAASSARLYWESFNTPSMAPITIPVGASIFPKE
                     IFRTSRRWAEKRFARLVHWNELERGGHFAAFEQPQLFVGELRACFRAMR"
     gene            complement(3477..3914)
                     /locus_tag="PLW13_06535"
     CDS             complement(3477..3914)
                     /locus_tag="PLW13_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70065.1"
                     /translation="MGDAGRDKGQQQVFASAMECERAFYQAFTARDLSAMEAVWADEE
                     PSCIHPGGALLRTRDEVLDSWAAIFDGAIAPQLRVHVVSRVSEGGLAVHLVREQISHG
                     EHTSHVLATNVFRRFDEGWRMIAHHGSVPPPKRDPAAAPRLLH"
     gene            4052..4372
                     /locus_tag="PLW13_06540"
     CDS             4052..4372
                     /locus_tag="PLW13_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70066.1"
                     /translation="MRSKLITVLVLGAALGAAPALGDGRGHKHGHRQHGYHYREPVRM
                     VRYERYVEVPPPRVYYGAYSPRPVYVHYDEPRRHRGDHDHDLIKWIGGAIVLGEIIHH
                     TSGH"
     gene            4388..4966
                     /locus_tag="PLW13_06545"
     CDS             4388..4966
                     /locus_tag="PLW13_06545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY70067.1"
                     /translation="MLMRAGGDGADGNATGAMDIEAFLRSIERRGFLMARLALGNDDD
                     ALDAMQDTMMRLVQRYANKPPAEWRPLFYRMLHNRITDTRRRRAIGSRLFGWLERRDD
                     DETPDPLERIADPAALDPGRIVASNETAEALMAAVAALPQRQQQAFMLRCWEGLSTAE
                     TATAMGCTEGSVKTHYSRAVHTLRERLGEHHA"
     gene            4963..5286
                     /locus_tag="PLW13_06550"
     CDS             4963..5286
                     /locus_tag="PLW13_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70068.1"
                     /translation="MNSFEEDARATLRAEENTLDGTTLTRLGAARRAALATPAAPWWR
                     LHAPALGGAALAVAVAVAVLIPAQEQLRAPTRAGGEPMAEDPQFYEDLDFYMWLSESE
                     MGNHG"
     gene            5279..5815
                     /locus_tag="PLW13_06555"
     CDS             5279..5815
                     /locus_tag="PLW13_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HQY70069.1"
                     /translation="MAERCARKAIVLVCALALAAGTMTAAPEQAQARGHDAGPMRLAR
                     NDDKGQARERGGGKQRAHDEQRGRDSSNGQDARERSQWRSADDGQARRAERNRSAQPA
                     QSPRREDPGAEQRRRLEQRQQSFRALPGEQQQRLRQAEERYRSMSPDQREDLRRRWES
                     MSESDRERYRRRIENRDD"
     gene            5921..7165
                     /locus_tag="PLW13_06560"
     CDS             5921..7165
                     /locus_tag="PLW13_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY70070.1"
                     /translation="MDVLLALLALFNVFAMITVFVPRTFPDSMLPWFVFAFALLSTEL
                     AWLWLPLQAVLALACVAGGALASTLGVIALLVLAISWLGLLRSIWLGLDSGTTVESAL
                     IQALGRDYRARIPAAACRGLRSVVRFADWRRPFAMQRPGVEVIRNVAYAQGGIRQRLD
                     IYRPSVIPPEGCPVLLQIHGGAWMIGAKEEQALPLMYRMAGSGWICVAANYRLSPSVG
                     FPTHLQDCKAALAWIRTHGRAYGMNPDFVAVTGGSAGGHLTALMGLTENRPDLQPGNE
                     GVDTSVQACVPFYGVYDFLNRSGARPSSAKFLEFLGERVLHATPEQNPALWELASPVT
                     QVHADAPPFMVLHGSRDSLAPVADARLFARHLREVSRNPVAYVELQGAEHAFDIMHSP
                     RTEHAIDGVQRFLEWVRAGQGR"
     gene            complement(7213..7389)
                     /locus_tag="PLW13_06565"
     CDS             complement(7213..7389)
                     /locus_tag="PLW13_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70071.1"
                     /translation="MGEVIDLRRPERHRGKTLCLNGHHKWKTWKEKQFDVRAGKLVTV
                     YRCERCGAQQVKAL"
     gene            complement(7419..7787)
                     /locus_tag="PLW13_06570"
     CDS             complement(7419..7787)
                     /locus_tag="PLW13_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY70072.1"
                     /translation="MISYVTVGTNDLPRASAFFDALLGEMGAARVMVMDRMVIWGAKP
                     GAPMLAVCTPYDGKPASAGNGTMVAIDAGSRETVDKLHAKALALGGSDEGAPGPRGDS
                     LYGGYFRDLDGNKFFACVFG"
     gene            complement(7862..8941)
                     /locus_tag="PLW13_06575"
     CDS             complement(7862..8941)
                     /locus_tag="PLW13_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY70073.1"
                     /translation="MPANASEKMRGLVYPWGAHAAPEPGTPVQVAEGVWWLRFPMPMA
                     LDHINLWLLEDRDGWTIVDTCLDIDTARALWESLFGGFMGGKPVKRVICTHLHPDHVG
                     LAGWLTRRFDCELWMSREEFLMCHTLADDTGRPAPEVAIRFYRAAGYDELALDNYRAR
                     FGMFGSHISPLPESFRRLVDGETIEINERYWQVIVGSGHSPEHAALYCPALKVLIAGD
                     QVLPRITPNVSVFPTEPKGDPLKEWLFSSARIRARLPDNVLVLPAHQAPFLGLHVRLT
                     QIIESHERGLAILFEALAEPRRAVDCSRVLFRRELSNDMLYMATGETLAHLNCLIGRR
                     MIRRERDAAGVDWYVQTPDALGPES"
     gene            9056..9511
                     /locus_tag="PLW13_06580"
     CDS             9056..9511
                     /locus_tag="PLW13_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HQY70074.1"
                     /translation="MTDPRISAQLPDWIKDHLQRYLASNGEDGHLWDATLAGGTGLVP
                     TLVLTTTGRKSGKPLTLPLIYGRTGNSYVVIASKGGAPQHPAWYLNLQANPEVEVQVK
                     ADRFGARARTATGAERAALWQQLVGIYHPYEKYQAATSREIPVVVLDPV"
     gene            9596..10471
                     /locus_tag="PLW13_06585"
     CDS             9596..10471
                     /locus_tag="PLW13_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70075.1"
                     /translation="MQEFAGQVAVITGGASGVGRSLAFALGRRGARVVAADVDRNAMD
                     ALERDASAMGIGLLTRRCDVTAGADCEALAGYAMNEHGRIDMVFANAGIGAGEAGPMW
                     TYSMKDWQWCFGVNVWGVVNTINAFLPRLVAQERKSHFTVTGSGNGAFVVLPDAPIYT
                     ATKAAVQAITENLYYQSVAMKSPVSVHALFPGPHVVDTGLFDSDRVRPESFDTGTPKS
                     SGIRSVEDMRRLMQQHGMELKTTHPDEVAEAAVAGILADTFWICPRTEESLAKFLARV
                     ESIRTLQNPVAASVG"
     gene            10571..11791
                     /locus_tag="PLW13_06590"
     CDS             10571..11791
                     /locus_tag="PLW13_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70076.1"
                     /translation="MDRYIVISSDCHAGLPGERYRDYLDPQYRETFDHAYPLQVEKIK
                     AAEKAFLIQEINDQWREGIEQQLTGAWDHAERLRMLDGDGIAGEIIFPDGITEMNTPP
                     FGAGLSLPTKDIVPELQWAGARAHNRWLAELCAQDPARHFGVAAIPLLWDVEEAVREV
                     RSIHASGLRGAMIPNMTGPFPHYHHRRYDPFWEACESLGVVVCFHSGAAPSEEFFGPG
                     WPTAHDPDYVGAMGIYVSEVLWWTYRPLTFLIWGGVFERYPKLKASFTETGCGWMLPP
                     YIRLLDHNYHDVQFSAKLGNFMGHLSISPSEYFRRNVAIGQSCMPRSDAEMRHEIGLK
                     QLMWGSDYPHPEGSWPKTRAHLQSTFSGLPDADIAAMLGDNAIDFYGFDRAALAPIAA
                     RIGPKRADFNTATQ"
     gene            11809..12822
                     /locus_tag="PLW13_06595"
     CDS             11809..12822
                     /locus_tag="PLW13_06595"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY70077.1"
                     /translation="MGKRYDFPVPFGWYAISLSRELAAGEVKPLHYFGKELVLFRTEG
                     GEARLLDAFCPHLGAHLGHGGKVKGEHIACPFHAWEFNGEGVCKHIPYAKNMPPRADG
                     KQTVFSYPVVERNQIIWAWYHPERIAPAFEVETVPEVGHPDWTEYRVYEWKFGAPIQE
                     AAENAADGAHFVYVHSSKDIPRGEITHEAHKRFARYVGKTPDMDEHGNIDRTGTRWRD
                     SLLETANFGPGQTLQRFMGLFDTIQLGLPTPIDSETMHLRFCFIQRKDMNATQQMMAK
                     ALTDEISRQVEQDMPIWEHKIFQPNPILCDGDGPIAQFRRWFSQFYANAAPEDSQRIR
                     AVS"
     gene            complement(12902..13135)
                     /locus_tag="PLW13_06600"
     CDS             complement(12902..13135)
                     /locus_tag="PLW13_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HQY70078.1"
                     /translation="MPTTLTIPKLQMSTTEGTLTEWLVEDGAPVTAGQAIYALETDKS
                     VQEIEAPESGRLIQKAAAGETYPVGTEIGEIVA"
     gene            complement(13153..>15051)
                     /locus_tag="PLW13_06605"
     CDS             complement(13153..>15051)
                     /locus_tag="PLW13_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQY70079.1"
                     /translation="TYWPMTGQEAIPASIAPLVTKDDYMVTIYRGIHDQVAKGVPLKG
                     LFAEALGRVGGVNKGKGGSPHISDPASGSMVTTAIVGAGAPIANGLALASKMRGTRAV
                     TIVNFGDGATSIGAIHEAMNLAGAWKLPVIFLCQNNQIGEYTPIPDYTAGENFHCRAD
                     ALGFKGVKLDGNDPVAFHNGMKSIIEDVRSGKGPVFVEAVTLRLGPHAGVGWSHLLKG
                     DALAAAKEAWPVPATRKLLLEKGICTEEELAAIDNAASAEVQEAIEWALGSEVTGEDE
                     MLLDVYGDPGVVPRRGQRDKRAAEAPHTGETRQMAMFEAVQDALDLALQLDDGVFCLG
                     EDIGDDPGGVFKCFKGMQSKWGERIRPTPIAEQAIIGAATGAALLGMRPVAEIMFADF
                     VGVCMDQITNHAAKQRYMSGATTHVPMTVRMIAGGGIGGFGAQHSQSTEAMLLHNPGL
                     KIVYPSTPTEAKGLLLSCIFDEDPCIQFESMMLTYALKEEVPVGDYRIPLGVAKVKRE
                     GTDVTLITYGWQVMQCLKAAEELAKEGISAEVIDLRTLLPLDYDRILTSVEKTGRALV
                     VHAATEFCGLGSEIASTINEALFHKLKAPASRFGAAYAPIAYSRIIETAQIPHAGTIA
                     ARVRELFK"
     assembly_gap    15052..15151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15152..17013)
                     /locus_tag="PLW13_06610"
     CDS             complement(<15152..17013)
                     /locus_tag="PLW13_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HQY70080.1"
                     /translation="MRNRITTSAVVLASALLAACERNTAVEYAPGPVAEWGAYGAAPG
                     GGHYSAADQITRDNVHALEQAWVYRSGDMREPGPGEIGGQKMPMLPGSSWQMTPILVG
                     DTLYGCSAFNRLFALDPSTGTEKWSYDPGVDVSQEIMVNCRGVSSWQAETPTGAACDH
                     RIIMGTLDGRLVAVDARDGKPCADFGGKGQVSLHEGLGEFAEQEYSVTSPPAILGDRI
                     ITGAMVLDRTHNHMPSGVVRAYNVRTGALEWYWDPIPPGQQPEFDAQGKPLYRRGTTN
                     VWSVISVDPERQLVFLPTGNTSTDFFGGLRDNMDYYSSSVVALKGDTGELAWHFQMVH
                     HDIWDYDTPAQPTLFELERDGRTIPALAQPTKMGHLFILNRETGEPLFPVEERPVPQD
                     WEVAGEYLAPTQPFPVKPAPLHPAGLTPDDAWGLTFWDRGACRKQLEALRNEGIFTPP
                     SLQGSAFFPSDFGGNNWGTPAIDPGRKLIILNTMFMPSMVQLIPREQCKLDAGDVFTA
                     PQLETPYCLRVKPLLSPLGAPCIAPPWGTLTAIDFTTGDKRWEVPLGNLEEMAPWPLS
                     RLIKGAPNIGGPSVTAGGLTFIASTPDYYLRAFDSETGEELWKAKLPTGGHATPT"
     gene            17195..>17442
                     /locus_tag="PLW13_06615"
     CDS             17195..>17442
                     /locus_tag="PLW13_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQY70081.1"
                     /translation="MRRVRLFIAPLLAVLASASANASPAPSPHDAIADAVNHYRTSKG
                     LAPLPLSASLMRVAQAHLADLEANYRRGGRCNMHSWSSR"
BASE COUNT         2805 a         5935 c         5818 g         2784 t
ORIGIN      
        1 acatgtaggt ccggcttcag ccggacagat gtaatgtccg gctgaagccg gacctacgcg
       61 agctcgtatt cgtccagctt cggctcgcgc gtccaggtcc agtagtcgag cagcttccac
      121 ggcgagagca cggtcacgcg gcccttgctg ttgcggtacc agctgtgcgc cacggcgggg
      181 tgcgaccaga cggtgcgcgc caacgtttcc tcgagacgcc ggttgtacgc gtcgtgcacc
      241 tcgcggcgca cctgcagcgt cttcgcatcg cgctcgagca tggccctgat gcaccccatg
      301 atatagcgcg cctggcactc gccgtggaag atcaggctgc cactggcggc gaggttcgtg
      361 gccgggccgt acatcacgaa caggttcggg aagtcgggca ccgtgatacc gaggtaggca
      421 gccggatcgt cgccccactg ctcgcccagc ttcacgccgt tgcggccggt gatctccatc
      481 ggccacaggt agcggttggc atggaagccc gtcgcgtaga ccacgacgtc ggcctcgaac
      541 tcgctgccgt ccgcggtgcg caccgccgtt tccgtcaggg caaccacctc gcggtcgacc
      601 agctgcacgt tgtcgcggcg cagcgcctgc aaccaggaac cgttgtcctg cagggtgcgg
      661 cgcgccagcg gcgggtacct gggcagcacg cgctcgaaca gcgcctcgtc ctcgccacac
      721 tgctggcgga tccagtccgt gaacatctcg cgcgtgaatt cgttgatcgc gttgatcgaa
      781 cgctcctggt gcggccactg cggatcgacg aaggtgcctt cccaggtgcc gtcggtggcg
      841 ggccagaaca gcaggaagcg gtaccagcgc ccgtagtagg gcaggtgacg gaagcaccag
      901 ctcgccccct cggacacggc gcggtggtaa tccgggttcg ggaacatcca ttgcggcgtg
      961 cgctggaacg tgtagacctg cgcggcctcc ttcgcgatct cgggcacgag ctggaaggcg
     1021 ctggcgccgg tgcctatcac cgcgacgcgc ttgcccgcga gactcacgga gtggtcccag
     1081 cacgccgaat ggaaggccgg cccgcggaag cgctcgagcc cctcgatgtc cggcagcttc
     1141 gggcggttca gctggcccac gccactgatc agcacgcgcg cgcgcagcgt ctgcgtcgcg
     1201 ccgtccttgg cgcgcacgcg cacgcgccat tcgccgctcg cctcgtcgaa gtccgccgcc
     1261 tgcacctcgg tgtcgaagcg gatgtgcggc tccaccccgt acttgcgcgc ggtgttctcg
     1321 aagtaacgcc gcagctcggg ctggcgcgcg aagaactcct gccagtcaag gttgggctcg
     1381 aaggaatagc agtagaggtg gttgcccacg tcgacgcggc agcccggata ggtgttctcg
     1441 taccaggtgc cgcccacgcc gtggttcttc tcgatgatca cgtagggaac gccttcctcc
     1501 tgcaggcgga tgcccgcgag gatcccggac atgccggcgc cgatgatcag caccggcagc
     1561 gcctcgcgcg cctgctgcgg gatgtgcgcc agcgagacct cgcgtgcgtc gcgcccgtcg
     1621 agctccatct cctcgagcat catcggcacg taggccgcgg gcacctcgcc ggcgaccagg
     1681 aagctcatca tgcgttgcag cgtgccggta tccggcgccg gcggcagcgt gcagccgcgg
     1741 tcgcggtagg ccttgatcac ctccagcgcg cggcggcgca gctcggcctg ctgcacggcg
     1801 ggcatcgcgc actgcatgtc gttcaggaag gccagcaccg ggcggatgtc gccgtcgagc
     1861 aggctcgcgt caccggtcat gtggatcatc gacatcatca ggcaggggac gctcgcctgc
     1921 tcgagcgccg ccgcgatcgt tgcgtcgtcg tcggtgatcg gttcgccggc cgggttgata
     1981 cggtgcgtca tggcgcgtgc tccggtgcaa tgcctgtgcg cgacattatc cgcccgtgcg
     2041 cgattcacgg ctgattacaa tcgcgcaagg cagccagtgg ggacgagggc ggcatggcct
     2101 gtgccagaca cgctgtaaat acgtccctgt acgctcgagc gccgcatccc tgcggcgcac
     2161 ggtctggcac aggccatgcc gccctcgtct tcgacgccga tgcgagctca gccccgagcc
     2221 atgcgatcct ccaggttgca cggctgtaac gacaagccgg gccggcctcg ggtaggcttc
     2281 ggcacaaaca ccagccatgg caacaggaga ccggaacatg tcggacgcca tcatcccttt
     2341 caaggtcgag atcgggagcg ccgggatcga ggacctgcgc cggcgcctcg cggccacgcg
     2401 ctggccggac gccgagacca cgggcgactg gagccagggc ataccgctcg cgtacatgca
     2461 ggaggtttgc acctactggg ccacgcagta cgacatgcaa cgcgtggcga agcgcctcaa
     2521 cgccttcccg cagttccgca cccggatcga cggcctcggc atccatttcc tgcacgtccg
     2581 ctcgccgcac gccgacgcgc tgccgctggt gatgacgcac ggctggccgg gctcggtcat
     2641 cgaattcctc aacgtgatcg aggcgctcac cgaccccacc cgccacggcg gcgaggcgaa
     2701 ggatgccttc cacgtcgtgt gccccgcgct tccgggctac ggcttctccg acaagccgcc
     2761 cgccaggggc tggggcgtcg aacgcatcgg ccgcgcctgg ggtgagctga tgcaacgcct
     2821 gggctacgca agctacgtgg cgcagggcgg ggactggggc tcgatggtga ccagcagcat
     2881 cgcgatgacc gaaaccgcgc attgccgcgg catccacctg aacatgccgc tggtcgcgcc
     2941 cgaccccgac acgatgagcg atctgagcgc gagcgaacag gctgcgctgg ccgccatgca
     3001 ggactattac gagaacgagt ccggctactc caagcagcag agcacgcggc cgcagacgct
     3061 gggctacggg ctcgccgact cgccggccgg gcaggccgcg tggatcctgg agaagttccg
     3121 ggcctggacc gactgcggcg agggcgaggc gaggcatccc gagaatgcgc tcacgcgcga
     3181 cgaactgctc gacaacgtga tgatgtactg gctgacggac tccgctgcct cgtcggcgcg
     3241 gctctactgg gagagcttca acacgccgag catggcgccg atcacgatcc cggtcggcgc
     3301 gagcatcttc ccgaaggaga tcttccgcac ctcgcggcgc tgggccgaga agcgcttcgc
     3361 ccggctggtg cactggaacg agctcgagcg cggcggccat ttcgcggcct tcgaacagcc
     3421 ccaactgttc gtgggcgagt tgcgcgcctg tttccgcgcg atgcgctgag cgcttctcag
     3481 tgcagtaagc gcggagccgc ggccggatcg cgcttgggcg gcggtaccga gccgtgatgc
     3541 gcgatcatcc gccagccctc gtcgaagcgg cggaacacgt tggtggccag cacatggctg
     3601 gtgtgctcgc cgtgcgagat ctgttcccgc accaggtgca ccgcgagccc gccctcgctg
     3661 acgcggctca ccacgtgcac gcgcaactgc ggtgcgatgg cgccgtcgaa aatggccgcc
     3721 cagctgtcca ggacctcgtc gcgcgtgcgc agcagcgcgc cgccggggtg gatgcagctc
     3781 ggctcctcgt ccgcccacac ggcttccatg gccgaaagat cgcgcgcggt gaacgcctgg
     3841 tagaacgcgc gctcgcactc catcgcgctg gcgaacactt gctgctgccc cttgtcccga
     3901 cccgcatcgc ccatccttac ctcctgccgc cgccctatgc cggcatggtg cgcgccgcga
     3961 cgacccgccg caatgccccg cgctgtcaac cgatcctgcc cgcaggcgtt ttctgcagca
     4021 atacggttca tcaccgggca aggagagacc gatgcgcagc aagctgatca ccgttcttgt
     4081 gctgggcgcc gccctcggcg ccgcgccggc gctgggcgac ggtcgtggcc acaagcacgg
     4141 ccaccggcaa cacggttacc attaccgcga gccggtacgc atggtgcgct acgagcgcta
     4201 tgtcgaggtg ccgccaccac gggtctacta cggggcgtat tcgccgcgcc cggtgtatgt
     4261 ccactatgat gagccccgtc gtcaccgggg ggatcatgat cacgacctca tcaagtggat
     4321 cggcggagcg atcgtgctcg gcgagatcat ccatcacacc agcggtcact gagcgccggg
     4381 cgacgggatg ctgatgcgcg ctggcggcga tggcgcggac ggcaacgcga cgggggcgat
     4441 ggatatcgaa gccttcctgc gctcgatcga gcgtcggggt ttcctgatgg cgcgcctggc
     4501 gctgggcaac gacgacgacg cactcgacgc gatgcaggac accatgatgc ggctggtgca
     4561 gcgttacgcg aacaagccgc ccgcggagtg gcgacccttg ttctaccgca tgctgcacaa
     4621 ccgcatcacc gatacgagac ggcgccgcgc gatcggttcg cggctgttcg ggtggctgga
     4681 gcggcgcgac gacgacgaga caccggatcc gctggagcgc atcgcggatc ccgcggccct
     4741 cgatccgggc cgtatcgtcg ccagcaacga gaccgccgag gccctgatgg ctgcggtggc
     4801 cgcgctgccg cagcgccagc agcaggcgtt catgctgcgt tgctgggaag gcctgtcgac
     4861 cgcggaaacg gccaccgcca tgggttgcac cgagggcagc gtgaagacac attattcgcg
     4921 cgccgtgcac acgttgaggg aaagactcgg agagcatcac gcatgaacag cttcgaggag
     4981 gatgcgcgcg cgaccctgcg cgccgaggaa aacacgctcg acggcaccac gctgacgcgc
     5041 ctgggtgccg cccggcgcgc ggcactggcg acacccgcgg cgccgtggtg gcgcctgcac
     5101 gcgccggcac tcggcggcgc ggcgctggcg gtggcggtgg cggtcgccgt gctgataccg
     5161 gcgcaggagc aactgcgagc gcccacccgt gccggcggcg aaccgatggc ggaggatccg
     5221 cagttctacg aggacctgga tttctacatg tggctctccg aatcggaaat gggcaaccat
     5281 ggctgagcgg tgcgcgcgca aggccatcgt gctcgtctgc gccctggcgc tcgccgcggg
     5341 cacgatgacc gccgcgccgg agcaggcgca ggcgcgcgga cacgacgcgg gcccgatgcg
     5401 gctcgcgcgc aacgacgaca agggccaggc ccgcgagcgc ggtggcggca agcagcgagc
     5461 gcacgacgag cagcgcgggc gcgacagcag caacgggcag gacgcccgcg agcgctcgca
     5521 atggcgctcg gcagatgacg ggcaagcccg gcgcgcggag cgcaaccggt ccgcccagcc
     5581 ggcgcagtcg ccgcgccgcg aagacccggg cgcggagcag cgccggcggc tggaacagcg
     5641 ccagcaaagc tttcgcgcgc ttccgggcga gcagcagcag cggctgcgcc aggccgagga
     5701 gcgctaccgc agcatgtcgc cagaccagcg cgaggacctg cgccgacgct gggagagcat
     5761 gagcgagagt gatcgcgagc gctaccgccg tcgcatcgag aaccgtgacg actgagcctc
     5821 gttcggccgc cgctcacgca gccattgcta tcgggctata gtgatagtcg tgcgcggcag
     5881 cgactgcgcg ccccaatcca gatcccggga gccgatgccg atggatgtgc tgctggcgct
     5941 gctcgcgctc ttcaacgtgt tcgccatgat caccgtcttc gtgccgcgca ctttccccga
     6001 cagcatgctg ccgtggttcg tgttcgcctt cgcgctgctc tcgaccgagc tcgcctggct
     6061 ctggctgccg ctccaggcgg tgctggcgct ggcatgcgtg gccggcggcg cgctcgcgtc
     6121 cacgctcggc gtcatcgcgc tgctggtgct ggcgatctcg tggctcggcc tgctgcgcag
     6181 catctggctg ggtctggaca gcggtaccac ggtggaaagc gcgctgatcc aggcgctggg
     6241 ccgcgactac cgcgcgcgta tcccggcagc cgcctgccgc ggactgcgca gcgtggtccg
     6301 cttcgccgac tggcgccggc ccttcgcaat gcagcgcccc ggcgtcgagg tcatccgcaa
     6361 cgtcgcctac gcgcagggcg gcatccgcca gcgcctcgac atctaccgtc cctcggtgat
     6421 tccgcccgag ggctgcccgg tgctgctgca gatccacggc ggcgcctgga tgatcggcgc
     6481 caaggaagag caggcgctgc cgctgatgta ccgcatggcg ggcagtggct ggatctgcgt
     6541 ggcggcgaac taccgcctga gccccagcgt cggtttcccc acccacctgc aggactgcaa
     6601 ggcggcactg gcgtggatcc gcacccacgg acgcgcgtat ggcatgaacc cggacttcgt
     6661 cgcggtcacc ggcggctcgg cgggcggcca cctgacggcg ctgatggggc tgacggaaaa
     6721 ccggcccgac ctgcagcccg gtaacgaggg cgtcgatacc tcggtgcagg cctgcgtgcc
     6781 gttctacggg gtctacgatt tcctgaaccg ctccggcgca cgcccgtcga gcgcgaaatt
     6841 cctcgagttt ctcggcgaac gcgtgctgca cgccacgccc gagcagaacc cggcgctgtg
     6901 ggagctggcc tcgccggtca cgcaggtcca cgccgacgcc ccgccgttca tggtgctgca
     6961 cggttcgcgc gacagcctgg caccggttgc cgacgcgcgc ctgttcgctc gccacctgcg
     7021 cgaagtgtcg cgcaacccgg tggcgtacgt cgaactgcag ggcgcggagc atgccttcga
     7081 catcatgcac tcgccgcgca ccgagcacgc catcgacggc gtgcagcgtt tcctggagtg
     7141 ggtacgggcg gggcagggcc gctgaacccg tgggtccggc ttcagccgga caatgtccgc
     7201 atgaatgcgg acctacaggg ccttgacctg ctgcgcgcca cagcgctcgc aacggtatac
     7261 cgtgaccagc ttgccggcac ggacgtcgaa ctgcttttcc ttccaggtct tccacttgtg
     7321 atggccgttc aggcacaggg tcttgcccct gtgccgctcg ggcctgcgca agtcgatcac
     7381 ctcgcccatg acggcgctac accgtgggca tcggcacttc agccgaagac acaggcgaag
     7441 aacttgttgc cgtcgaggtc gcggaaataa ccgccgtaca gcgagtcgcc gcgcggtccc
     7501 ggcgcgcctt cgtcactgcc cccgagcgcc agcgccttcg cgtgcagttt gtcgacggtc
     7561 tcgcggctgc cggcatcgat tgcgaccatg gtgccgttgc ccgcgctggc gggcttgccg
     7621 tcgtagggtg tgcagaccgc gagcatcggc gcgccgggct tggcccccca gatgaccatg
     7681 cggtccatca ccatgacgcg cgcggcgccc atctcgccga ggagcgcgtc gaagaaggcg
     7741 ctggcgcggg gaaggtcatt ggtgccgacg gtgacatagc tgatcatgga aatctcctgt
     7801 tgtgctcggg gggataatgc aggcgggcca ttattcggga gattcgttgc cagcggaagg
     7861 gtcaggactc aggccccagc gcatcgggcg tctgcacgta ccagtcgacg cccgcggcgt
     7921 cgcgctcgcg gcggatcatg cgccggccga tcaggcagtt cagatgcgcc agcgtctcgc
     7981 cggtggccat gtagagcatg tcgttgctga gctcgcggcg gaacagcacg cgggagcagt
     8041 ccaccgcgcg cctgggctcg gcaagggcct cgaacaggat cgcgagcccg cgctcgtgcg
     8101 actcgatgat ctgcgtcagg cgcacgtgca agccgaggaa cggcgcctgg tgcgcgggca
     8161 gcaccagtac gttgtcgggc agcctggcgc ggatgcgcgc gctcgagaac agccattcct
     8221 tcaacggatc gcccttgggc tccgtgggaa acacgctgac gttcggggtg atgcgcggca
     8281 gcacctggtc gccggcgatc aggaccttca gcgccgggca atacagcgcg gcgtgctccg
     8341 gcgagtggcc gctgcccacg atcacctgcc agtagcgctc gttgatctcg atggtctcgc
     8401 catcgaccag gcggcgaaaa ctttcaggca gcggcgagat gtgcgagccg aacataccga
     8461 agcgggcgcg gtagttgtcg agcgccagct cgtcgtaacc ggccgcgcgg tagaagcgga
     8521 tcgcgacctc gggggccggg cggccggtgt cgtcggccag cgtgtggcac atcaggaact
     8581 cctcgcgcga catccacagc tcgcagtcga agcgccgcgt cagccagccg gcgagcccga
     8641 cgtgatccgg gtgcaggtgc gtgcagatca cgcgcttcac cggcttgccg cccatgaaac
     8701 cgccgaacag cgattcccac agcgcgcgcg ccgtgtcgat gtcgaggcag gtatcgacga
     8761 tggtccagcc gtcgcggtcc tcgagcagcc acaggttgat gtggtcgagc gccatcggca
     8821 tcgggaagcg cagccaccac acgccctcgg cgacctgcac cggcgtgccg ggctcgggtg
     8881 ccgcgtgtgc gccccaggga tagaccaggc cgcgcatctt ttcgctggca ttcgcaggca
     8941 tcggtgcaat tccgtactgc tgtggatcgc gccatgttaa cccggaacac gatcggctcg
     9001 cgccggcacc cggcgcgtgg cagtctagtg gcccccgtca cccaggagcc gtgccatgac
     9061 cgacccacgc atttccgcgc aactgcccga ctggatcaag gatcacctgc agcgctacct
     9121 cgccagcaac ggcgaggacg ggcacctgtg ggacgccacg ctggccggcg gcaccggcct
     9181 ggtgcccacg ctggtgctga ccacgacggg ccgcaagtcc ggcaagccgt tgacgctgcc
     9241 gctgatctac gggcgcaccg gcaacagcta cgtcgtgatc gcctccaagg gcggtgcgcc
     9301 gcaacacccg gcctggtacc tgaacctcca ggccaacccg gaagtcgaag tgcaggtgaa
     9361 ggccgatcgc ttcggggccc gcgcgcgcac cgccaccggc gcggaacgcg ccgcgctgtg
     9421 gcagcagctc gtcgggatct accacccgta cgagaagtac caggccgcaa cctcgcgcga
     9481 gattcccgtg gtggtgctcg accccgtgtg aacccggccg ccttaccgat gattcacttg
     9541 ccctcggtgc gaccatccca ataatgggtc gcctgtcaac agaaggacgg cgctcatgca
     9601 ggaattcgca ggccaggtcg cggtcatcac cggtggagcg agcggggtgg gccgctcgct
     9661 tgcctttgcg ctcggacggc gcggcgcgcg ggtagtcgcg gccgatgtcg acaggaacgc
     9721 gatggatgcg ctcgagcgcg acgcgagcgc gatggggatc ggcctgctga cacggcgctg
     9781 cgacgtcacc gccggcgccg actgcgaggc gctggccggg tacgccatga acgagcacgg
     9841 ccgcatcgac atggtgttcg ccaacgcggg catcggcgcc ggcgaggcgg gaccgatgtg
     9901 gacctactcg atgaaggact ggcagtggtg cttcggggtc aatgtatggg gcgtggtgaa
     9961 caccatcaac gccttcctgc cgcgcctggt cgcgcaggag cgcaaatcgc acttcacggt
    10021 caccggctcc ggcaacggag ccttcgtggt gctgcccgac gcgccgatct ataccgccac
    10081 caaggcagcg gtgcaggcga tcaccgagaa cctctactac cagagcgtcg cgatgaagag
    10141 cccggtcagc gtgcacgcgc tgttccccgg gccccacgtg gtcgacaccg ggctgttcga
    10201 ttccgatcgg gtgcgacccg agagcttcga cacgggcacg ccgaagtcct ccggtatccg
    10261 ctcggtggag gacatgcgcc gcctgatgca gcaacacggc atggagctga agaccacgca
    10321 tcccgacgag gtcgccgaag ccgcggtggc cggcatcctg gcggacacgt tctggatctg
    10381 tccgcgcacc gaggagtcgc tggccaagtt cctcgcccgc gtggaatcga tccgcacgct
    10441 gcagaatccg gtcgcggcct cggtgggctg aacccgccga gatcatgtcg gcatgaatgc
    10501 cgacccacgg cagcacgccg ggatcaagtc tcggcatgaa tgcccaccga cagcatcagg
    10561 agcaccgtga atggaccgct acatcgtcat ttcgtccgac tgccacgccg gcctgcccgg
    10621 cgagcgctat cgcgattacc tcgatccgca gtaccgcgag accttcgacc acgcgtaccc
    10681 gctgcaggtg gagaagatca aggcggccga gaaggccttc ctgatccagg aaatcaacga
    10741 ccagtggcgc gagggcatcg agcagcagct caccggcgcc tgggaccacg ccgagcgcct
    10801 ccgcatgctc gacggcgacg gcatcgccgg ggaaatcatc ttccccgacg gcatcaccga
    10861 gatgaacacg ccgcccttcg gcgcgggcct gagcctgccg accaaggata tcgtgcccga
    10921 actgcagtgg gccggcgcgc gcgcccacaa tcgctggctc gccgagttgt gcgcgcagga
    10981 tccggcgcgg cacttcggcg tcgcggcgat accgctgctg tgggacgtgg aggaggccgt
    11041 gcgcgaggtg cgcagcattc acgcgagcgg cctgcgcggt gcgatgatcc cgaacatgac
    11101 ggggccgttc ccgcactacc accaccgccg ctacgacccg ttctgggaag cctgcgagtc
    11161 gctgggcgtc gtggtctgct tccactcggg cgccgcgccc agcgaggagt tcttcggccc
    11221 cggctggccg acggcgcacg acccggacta cgtgggcgcg atgggcatct acgtctcgga
    11281 agtgctgtgg tggacctacc gcccgctcac cttcctgatc tggggcggcg tgttcgagcg
    11341 ctacccgaaa ctcaaggcca gcttcaccga gaccggctgc ggctggatgc tgccgcccta
    11401 catccggctg ctcgatcaca actaccacga cgtgcagttc tcggcgaagc tcggcaactt
    11461 catgggccac ctgtcgatct cgccgagcga gtacttccgc cgcaatgtcg cgatcggcca
    11521 gtcgtgcatg ccacgctccg acgccgagat gcgccacgag atcggcctga agcagctgat
    11581 gtggggcagc gactacccgc atccggaggg ctcctggccg aagaccaggg cgcacctgca
    11641 gtcgaccttc tcggggctgc cggacgcgga catcgccgcg atgctgggcg acaacgcgat
    11701 cgacttctac ggcttcgacc gtgctgcgct ggcacccatc gccgcgcgca tcgggccgaa
    11761 gcgcgccgac ttcaacaccg ccacgcaatg acacccggga gaccgaccat gggcaagcgt
    11821 tacgattttc ccgttccctt cggctggtac gcgatcagcc tgtcgcgcga gctcgcggcc
    11881 ggcgaggtca agccgctgca ctacttcggc aaggagctgg tgctgttccg caccgagggc
    11941 ggcgaggcac ggctgctcga cgccttctgc ccgcaccttg gcgcacacct cggccacggc
    12001 ggcaaggtga agggcgagca catcgcctgc cccttccacg cctgggagtt caacggcgag
    12061 ggcgtgtgca agcacatccc ctacgcgaag aacatgccac cgcgcgccga cggcaagcag
    12121 accgtgtttt cctaccccgt ggtcgagcgc aaccagatca tctgggcctg gtatcacccg
    12181 gagcgcatcg cgcccgcctt cgaggtcgag acggtcccgg aggtcggtca ccccgactgg
    12241 acggagtacc gcgtctacga gtggaagttc ggcgccccga tccaggaggc cgccgagaac
    12301 gccgccgacg gggcacactt cgtgtacgtg cacagctcga aggacatccc gcgcggggaa
    12361 atcacgcacg aggcccacaa gcgcttcgcg cgctacgtcg gcaagacgcc cgacatggac
    12421 gagcacggca acatcgaccg caccggcacg cgctggcgcg acagcctgct cgagaccgcc
    12481 aacttcggcc ccggccagac cctgcagcgc ttcatggggc tgttcgacac gatccagctc
    12541 ggcctgccga cgccgatcga cagcgagacc atgcacctgc gcttctgctt catccagcgc
    12601 aaggacatga acgccacgca acagatgatg gccaaggcgc tgaccgacga gatctcgcgc
    12661 caggtcgagc aggacatgcc gatctgggag cacaagatct tccagcccaa ccccattctc
    12721 tgcgacggcg acggcccgat cgcgcaattc cggcgctggt tcagccagtt ctacgcgaac
    12781 gccgcgcctg aggacagcca gcgtatccgc gcggtgtcct gaggtgctct agcgggacgt
    12841 tacgcccgcg gcacgctcac cgcgaccgcc cggcgtagcg tgccccgggc ggtcgacggt
    12901 gtcaggcgac aatctcaccg atctccgtgc cgaccgggta ggtttctccc gccgctgctt
    12961 tctggatcag cctgcccgac tccggcgctt cgatctcctg gaccgacttg tcggtttcca
    13021 gtgcatagat ggcctgcccg gccgtcaccg gtgctccgtc ttccaccagc cattccgtca
    13081 gggttccctc ggtggtggac atctggagct ttggtatggt caatgtcgtc ggcatatgta
    13141 ttccctggag cgctatttga aaagttcgcg aacgcgcgcg gcgatggttc cggcgtgcgg
    13201 aatctgcgcg gtttcaatga tcctggagta ggcaatcggt gcgtaggcag caccgaacct
    13261 ggaggccggt gccttgagct tgtggaacag cgcctcgttg atggtgctgg cgatttccga
    13321 gcccagacca cagaattccg tggcggcatg aaccaccagc gcgcgaccgg ttttctccac
    13381 tgacgtcagg atgcggtcgt aatccagcgg cagcagggtg cgcaggtcga tcacctcggc
    13441 gctgatacct tccttcgcca gttcttccgc cgccttcagg cactgcatca cctgccagcc
    13501 ataggtgatc agggtgacgt cggtaccttc gcgcttgacc ttggccacac ccaacgggat
    13561 acggtagtcc cctaccggta cttcctcctt cagggcatag gtcagcatca tggattcgaa
    13621 ctggatgcag ggatcctcgt cgaagataca gctcagcagc aggcccttgg cttcggtggg
    13681 ggtgctgggg taaacgatct tcaggccggg gttgtgcagc agcatggcct cggtggactg
    13741 ggagtgctgg gcaccgaagc caccgatgcc gccgccggca atcatgcgca cggtcatggg
    13801 cacatgggtg gtggcaccgg acatgtagcg ctgcttggcg gcgtggttgg tgatctggtc
    13861 catacagacg ccgacaaaat cggcgaacat gatctcggcg accgggcgca tgcccagcag
    13921 ggctgcaccg gtagcggcac cgatgatggc ctgctcggcg atgggtgtcg gcctgatgcg
    13981 ctcgccccac ttgctctgca tgcccttgaa acacttgaaa acaccgcccg ggtcatcgcc
    14041 gatatcctcg ccgaggcaaa acacgccgtc atcgagctgc aacgccaggt cgagggcatc
    14101 ctgtaccgcc tcgaacatgg ccatctggcg ggtctcaccg gtgtggggtg cctccgccgc
    14161 gcgcttgtcg cgctggccgc gacgcggtac cacgcccgga tcgccataga cgtccagcaa
    14221 catctcgtct tcgccggtta cttcactgcc cagcgcccac tcgatcgctt cctgcacttc
    14281 ggcactggcg gcattgtcga tcgcggcgag ttcctcctcg gtacagattc ctttttccag
    14341 caacagcttg cgggtggcgg ggacgggcca ggcttccttt gccgccgcca gtgcgtcacc
    14401 tttgagcagg tgggaccagc ccacaccggc atggggcccc aggcgcaggg tgaccgcttc
    14461 gacgaacacc gggcccttgc cgctgcgcac gtcctcgatg atggatttca tgccgttgtg
    14521 gaaagccacc ggatcgttgc cgtcgagctt gacgcccttg aagcccaggg cgtcggcgcg
    14581 gcagtggaaa ttctcgccgg cggtgtagtc ggggatcggc gtgtattcac ctatctggtt
    14641 gttctggcaa aggaaaatga cgggcaactt ccaggcgcca gcaaggttca tggcttcgtg
    14701 gatcgcgccg atgctggtgg caccgtcgcc gaagttgaca atggtcaccg cccgggtgcc
    14761 gcgcatcttg ctggccaggg ccaggccgtt ggcgatgggg gcgcccgcac cgacaatggc
    14821 agtggtcacc atggaaccgg aggcgggatc ggagatgtgg ggcgagccgc ccttgccctt
    14881 gttgacgccg cccacccgtc cgagagcctc ggcgaagagg cctttcagcg gtacgccctt
    14941 ggcgacctga tcgtgaatcc cccgatagat ggtgaccatg taatcgtcct tggtcaccag
    15001 cggggcaatg gacgcgggaa tggcttcctg tccggtcatc ggccagtacg tnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcggcgtg gcgtgccccc cggtgggcag
    15181 cttggccttc cacaactcct cgccggtctc gctgtcgaag gctcgcaggt agtagtcggg
    15241 cgtggacgcg atgaacgtca ggccgccggc ggtcacgctg gggccgccga tgttgggcgc
    15301 gcccttgatc agccgcgaca gcggccacgg cgccatttcc tcgaggttgc cgagcggcac
    15361 ctcccagcgc ttgtcgccgg tggtgaaatc gatggcggtg agcgtgcccc agggcggagc
    15421 gatgcacggc gcacccagcg gcgacagcag cggcttcacc cgcaggcagt acggtgtttc
    15481 gagctgcggc gcggtgaaca cgtcgcccgc atcgagcttg cactgctcgc gcgggatcag
    15541 ctgcaccatc gagggcatga acatggtgtt caggatgatc agcttgcgcc cgggatcgat
    15601 cgccggcgtg ccccagttgt tgccgccgaa atcgctgggg aagaaagccg aaccctgcag
    15661 cgaaggcggc gtgaagatgc cttcgttgcg cagcgcctcg agctgcttgc ggcaggcacc
    15721 gcggtcccag aaggtgagcc cccaggcgtc gtccggcgtc aggccagccg ggtgcagcgg
    15781 tgccggcttt accgggaagg gctgcgtcgg cgcgaggtat tcaccggcca cttcccagtc
    15841 ctgcggcacc gggcgttcct cgaccgggaa cagcggctcg ccggtttcgc ggttcaggat
    15901 gaacaggtgt cccatcttgg tcggctgcgc cagcgccggg atggtcctgc cgtcgcgctc
    15961 gagctcgaac agcgtgggtt gcgccggggt gtcgtagtcc cagatgtcgt ggtgcaccat
    16021 ctggaagtgc caggcgagct ccccggtatc gcccttcagg gcgaccaccg agctggagta
    16081 gtaatccatg ttgtcgcgca agccgccgaa gaaatcggtc gaagtattac cggtgggcag
    16141 gaacaccagc tggcgctcgg gatcgacgga gatcaccgac cacacattgg tggtgccgcg
    16201 acggtacagc ggcttgccct gggcgtcgaa ctccggctgc tgtccgggcg ggatcgggtc
    16261 ccagtaccac tcgagcgcgc cggtgcgcac gttgtaggcg cgcaccacgc cgctcggcat
    16321 gtggttgtgc gtgcggtcga gcaccatggc gccggtgatg atccggtctc cgaggatcgc
    16381 cggcggcgag gtcaccgagt attcctgctc ggcaaactcg cccagcccct cgtgcaggct
    16441 cacctggccc ttgccgccga agtccgcgca cggcttgccg tccctggcgt cgactgcaac
    16501 gagccggcca tcgagcgtgc ccatgatgat gcggtggtcg caggccgcgc ccgtcggtgt
    16561 ttcggcctgc cagctgctga cgccgcggca gttcaccatg atctcctggc tcacatcgac
    16621 acccgggtcg tagctccatt tctcggtgcc ggtcgaagga tcgagcgcga acaaccggtt
    16681 gaaagcgctg cagccgtaca gcgtgtcgcc gaccaggatc ggtgtcatct gccagctgct
    16741 gccgggcagc atcggcatct tctgcccgcc gatttcaccg ggacccggct cgcgcatgtc
    16801 gccgctgcgg tacacccagg cctgctccag tgcatgcacg ttgtcgcgcg tgatctggtc
    16861 ggccgcggag taatgcccgc cgcccggcgc ggcgccgtag gcgccccatt cagccaccgg
    16921 acccggtgca tactccaccg cggtgttgcg ctcacaggcc gcgagcagcg cgctcgccag
    16981 cacgacggcc gatgtggtga tacggttacg caagcgagtc tcccgaggtt cgtgcgcgtt
    17041 tcggaggaaa tgcgctattt tgttttcatt cgtgcgcacc gcatcgcggc gaacgcagtc
    17101 ggtcagagtg ccgagcgcgc tgcgagaatg caagttgtgg aacggccgcc cggggacgca
    17161 ccatttcccc accacatcac aggaccctct atacatgcgc cgcgttcgtc tcttcatcgc
    17221 accgttgctc gcagtcctcg cctcggcgag cgcgaacgcc tcacccgcgc cgtcaccgca
    17281 tgacgccatc gccgacgccg tgaatcacta ccgcacgagc aagggcctgg ccccgctgcc
    17341 gctgtcggcc tcgctcatgc gcgtggcgca ggcgcacctg gccgatctcg aggcgaacta
    17401 ccgtcgcggc gggcgctgca acatgcattc gtggtcgagc cg
//
LOCUS       DAORUQ010000053        17263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_2991_length_17263_cov_5.456764, whole genome shotgun sequence.
ACCESSION   DAORUQ010000053 DAORUQ010000000
VERSION     DAORUQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_17263_cov_5.456764"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..973
                     /locus_tag="PLW13_06620"
     CDS             26..973
                     /locus_tag="PLW13_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70082.1"
                     /translation="MQNCVFIQTNHKQITGAIVAQHALRRYSTHNDQFDVKIIEKKDY
                     PYFDAREGQTYLRDGVKRVWLNDDLQSFTLTRFMPPELMGFQGRAVVIDPDVFACADV
                     WELFSRDMQGKAVMCRPRSGTKGLIDKCLATSVMLLDCAKLQHWKVQERFNRMFDFTF
                     DYMDWICLKSEPEGTVGLLEHEWNDFDKLTPATKMLHTTRRRTQPWKTGLPVDFRIAE
                     RFRLFPPAAWLMKARRHLFGEYGLMGHYRKHPDINQQNFFFGLLKECLDNGMVTESFL
                     REEMRLNHVRHDAIEVLKSTPDLPPAPRHPLSGVAQKAA"
     gene            complement(989..2068)
                     /locus_tag="PLW13_06625"
     CDS             complement(989..2068)
                     /locus_tag="PLW13_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HQY70083.1"
                     /translation="MNEDNAHSSLTPRVWLLLGHKAGDNNQVLALAEALAWPWEEKRI
                     VYRPWELLANRLLGVTLCGIDRGASSALQPPWPDLVISSGRRNEPVGRWIRRQAPGVR
                     LVHVGRPWAPLDVFDLIVTTPQYSLPLRPNVLENELPMHRLRRERLATEAQRWRARLA
                     PLAVPRIAVLLGGNSGAYVFTPEKARRLGRLVNGLARSQHGSVLVTDSARTPPGVTDA
                     FLAELDVAVHCHRWGAAREDNPYLGYLGLAEQFVVTADSMSMIAEALFAAKPLFLFSL
                     EDGPHWWKRRYNYRFNALAHRLAMAIGPRRMRRDTGRIIGRLVADGRASWLGEGKAAM
                     SARDPVDEAERAAAAVAALFRANGA"
     gene            2234..3307
                     /gene="lptF"
                     /locus_tag="PLW13_06630"
     CDS             2234..3307
                     /gene="lptF"
                     /locus_tag="PLW13_06630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HQY70084.1"
                     /translation="MLIERYITSEIVRPFAAGLAILAAVFIAFSAAVKLSEAASGEIA
                     AHVVAQLIFLNTLIAMEVLVPTTLFLAILFAIGRLYRDSEMAALSAAGVSEASILRAV
                     GKLGLLCAIAVALLSVFARPWAYARTYALEQQTISRFDLSNIRAGTFTDVGNGGYVLY
                     AREVDVEADEMRGVFLQVEREQRSQVISAERARIHAMDEEGSRSVEFFDGHAYLLERG
                     GTRDIDMRFNSFLVRLPEEERISKFRRKAVATGTLGDSDQPKDIAEYQWRLTTPLATL
                     VLALLAVPLSRASPRQSRTGIFAVAVAVYLLVFSVSGVVRNALENGDIPPFPGLLLAY
                     VPPVALLVLLMLMPGLRRRGWRR"
     gene            3304..4380
                     /gene="lptG"
                     /locus_tag="PLW13_06635"
     CDS             3304..4380
                     /gene="lptG"
                     /locus_tag="PLW13_06635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HQY70085.1"
                     /translation="MIIRRFLAGQLARGWFIVFLVLTALFGLLALVDEIDNLSERYRL
                     AHALHYVLLTTPQRVLELSPVIAALGTILAFATLTRNSELVVIRGAGFSLRQLLQICA
                     VPTLAMMLALGFAGEFLVADMHQRAETERMVRRSGNLDLLDGQGLWSKSGNRFFNVRN
                     LRAGHIPEQISLYEFGPDNELLRAIDARGAELVGEREWKLIDVQFKEWQDGRVSTRNL
                     PSLELGPFWSAAELPALGQSLAAMSPRALYAYANHLEQSGADDGQVRMAFWQKAMLPF
                     SFAAMVLLSVVIGVGFGTTRSAAFGWRVLSGAVIGVGFYLLTQIFHTGGQLLGLGQSV
                     VVLIPICLVVLAAAVVARITRGPR"
     gene            4399..6102
                     /locus_tag="PLW13_06640"
     CDS             4399..6102
                     /locus_tag="PLW13_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY70086.1"
                     /translation="MRLMLSFYRAYPWQTFFTLLALSVAGLAEGVGLSVLLPLLNMAM
                     EGQGQAAPASDNEFSQSVRGALGVLGIAPSLGNMLLIVVFAIGCKSLLLLFAKRQVGY
                     TAARLATDLRLELLRAIMRSRWEYFLGQPVGKLTNALATEAARASESFVNGATALTFL
                     AQAVVYGAVAIAVSWRATLTGIVAGAVIIGLSHSLVRITKKAGRKQTSVLLSLMATMT
                     DTLGSVKPLKGMGREHLADAVLSMETTRLNRALERQALGSALLESTQEFLFALIIAGG
                     IYVALAKFAMPFATVMVLVVVVGQMVALLGKVQKQYQKMTLSESAYWSMQRTIAEAQA
                     EEEELRGGERPVLATRLCMEGVCFAYDERRVLDDLSLEIPAGELVTLIGPSGAGKTTV
                     VDLLIGLIRPDAGRITLDGTPLDSMDIKQWRRSIGYVPQETLLLHESIAHNVSLGDSE
                     LSQADIEHALRAAGAWDFVSALPEGMHSNVGERGGRLSGGQRQRIVIARALVRRPQLL
                     ILDEATSALDAESAEAVRQTLERLRGSLTILTITHESGLVDIADRVYRLESGRAVRQS
                     P"
     gene            complement(6099..7166)
                     /locus_tag="PLW13_06645"
     CDS             complement(6099..7166)
                     /locus_tag="PLW13_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HQY70087.1"
                     /translation="MPEPNTNTPPRVWVIAAYRAGEQSQLLALAEALGWPFEVKQLRH
                     RWQGALFNLVRGTGLMGIDVAASSALVAPWPDVVIAAGMRNEPVCRWIRRASGGMTRI
                     VHIGRPWADAHNFDLVITTPQYRLPEDPRVLQNTLTLHRVTPQRLAAAREQHGARLAA
                     LPSPRIAVIVGGPSGPYAFGPRAGARLGRQASRLAEALGGSLMITTSARTPRAALAAL
                     RASISAAHELYEWKAGDAQNPYFAYLACAERLIVTFDSISMLSEACATGRPVYMFDLA
                     REHGAGAPQGAADRSLRSLGYALLMRFGPRRLGRDIGLVHEQLLRDGRAAWLGEPGEP
                     RPGAAGADIERAVARVKALLA"
     gene            7280..7525
                     /locus_tag="PLW13_06650"
     CDS             7280..7525
                     /locus_tag="PLW13_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY70088.1"
                     /translation="MARYEPCLARVLEILGALGKAKTTITEASDLAGELGLSSIDVME
                     VIEQVEDEFDISFPLNSLSGIHTVSDLAREITRLSTS"
     gene            7522..8703
                     /locus_tag="PLW13_06655"
     CDS             7522..8703
                     /locus_tag="PLW13_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY70089.1"
                     /translation="MKLLDKFQAMAGVRAELDGLGIRPFGAVTEKILSPTEAIVDGRR
                     VILAGTNNYLGLTFDPECIAAAQRALAEQGTGTTGSRMANGTFAEHVALERELAAFYG
                     RRHAIVFSTGFSATMGMGATLAGSGDVVMLDADSHASIYDGVRLGGAEIIRFRHNDVE
                     NLERKLARLGERAANTLVILEGLYSMLGDIAPLAEIAQLKKQYDFCLMVDEAHSLGVL
                     GERGRGLAEAAGVEEAVDFVVGTFSKSLGSIGGYCVSNHAQLEAIRFSIRSYIFTASP
                     SPAVIASTRVALRLMQERPQLRTRLWHNAERLYAGLQGLGFTLGPQASPVVAVRIEDR
                     ETALRWWPQLLEQGIYVNLVVPPASPSAFSLLRCSVSAAHSDDQVDRIIAAFATLRTG
                     A"
     gene            8700..9650
                     /locus_tag="PLW13_06660"
     CDS             8700..9650
                     /locus_tag="PLW13_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY70090.1"
                     /translation="MSEAAADAPAFRFAQISDPHLSDLSGVRWSQLASKRVLGYLSWR
                     RRRRHEHRPEMLAALSADLHAIAPGHTVVSGDLTHIALPGEFREASDWLRQLGPAEAV
                     TVVPGNHDRYVRTPWAESLGLWSSYMSSDDGSAEHGAEQLFPLLRVRGPLAFIGLDSA
                     VPSPPFMATGRLGKRQIERLAAILERLRGSGLLRVLVLHHPPVPGEEKWRKRLVDAPA
                     LCALLARHPVDMVLHGHRHRALQSVIRIPGSEIPVFGIPSASAAGVLSNYPAEYNLYD
                     IIRDGASWRVRVSARRFDAATRAFAGRELASFALRNDASR"
     gene            complement(9660..10475)
                     /locus_tag="PLW13_06665"
     CDS             complement(9660..10475)
                     /locus_tag="PLW13_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQY70091.1"
                     /translation="MNGNDRNRPFAALVLAGDRSSSDPLVAASGRGCKALVEIDGTPM
                     ILRVLDALGHSAEVGEITICGPSRESIATEARLQHLIAEQRIGWQPPQATPSTSAWQA
                     LAATPSATPVLLTTADHPLLRSDIIDHFCREALATGADAVVGLAPYALVRELFPAMRK
                     TVLRFSDDEYCGCNLFAFLTPAGRQMAERWRAVEAERKSPLKVMKMLGWLSVLRYRFG
                     WLSLAGAERALSRRMGLRLAAVRMPFGDAAVDVDSLSDHELVQQRLRALRGAA"
     gene            complement(10479..11615)
                     /locus_tag="PLW13_06670"
     CDS             complement(10479..11615)
                     /locus_tag="PLW13_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQY70092.1"
                     /translation="MSMHPPITVQAADSAGLRREFLRLPHQLYLNDPHWVAPLEVELR
                     ERLWGGNPFLQHASAQAWVAYAQGRAVGRISAQFDRLQQEYHDDMSGSFGLIEAEDDP
                     AVVAALLDTASAWLRAHGARRVRGPLNLSVNEECGLLVEGFDTPPSIMMGHARPYYDA
                     LLRGCGLAPARDLLAYRVAPDFEAPRVMRRLAESLSGQVRVRPMIRRDKAHELGILRD
                     IFNDAWSQNYGFVPFTVEEFNDVGKLMTLLLPDDYVQIAEVDGEPAAFIVAMPNINEV
                     TAGLRGRLLPLGWLRLLWGLKVRHPQSARVPLMGVRKKFQQSRLGPGLAFMVIDAVRK
                     GLCARGVREVELSWILEDNSGMRSIIEAIGGQVYKRYRIYEKAI"
     gene            11989..13761
                     /locus_tag="PLW13_06675"
     CDS             11989..13761
                     /locus_tag="PLW13_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HQY70093.1"
                     /translation="MHTESTPTRHSLPFRGADFTTLAEALDYAADGATGTNFYSGRGQ
                     LYATLSYTDLRAQCLVLARKFTGLGLVKGDRVAMVAETHPDFLRVFFACQYAGLIPVA
                     LPATINLGGHQSFVSQLRGLIENSGAAVTIASEAFMPFVVEATEGLELRMIGEPAEFD
                     ALPESPDALPAIGPDDVSYLQYTSGSTRFPRGVIITHRSVMSNLSGIIAHGVKIGEGD
                     RCFSWLPFYHDMGLVGLVLVPVASQMSVDYMDTRDFAMRPRQWLALMSQTKATISFSP
                     SFGYELCARRLREGEAARYDLSNWRVAGIGAEMIRWETLDRFAEALAPAGFDARAFLA
                     CYGMAECSLAVSFAPLFTGYQTDRIDLDELADNGKALPVAQGDESRRTRAFVKCGEPL
                     PEYEMEIRDERGALLPDRHTGTIFVRGPSVMSGYFNAPQDIGGALSADGWLNTGDIGY
                     SVEGSLVITGRQKDLIIINGRNIWPQDLEYLAEHQDETRVGDALAFSVPGPESREVCV
                     LVVQCRETNANRRAALIDRLTRLVRIEMGIDCYVELVPPHTLPRTSSGKLSRSKARQN
                     FMDSHDLAEVAAIAEGMSLKQASA"
     gene            13792..14721
                     /locus_tag="PLW13_06680"
     CDS             13792..14721
                     /locus_tag="PLW13_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY70094.1"
                     /translation="MRVAITGATGFIGRRIASLLESPQYLVRALARDAASARHLLAPG
                     VEIVPGDLATPQALRALVRDADAVLHLAGAVRGATRADFDAANVDGSAALLAAMDAAA
                     PHARLLFFSSLAAREPALSHYAGSKQRAEQLLLRRAAARPVTILRPPAVYGPGDREML
                     PVFRFMARTGRAPCAGRAADRLSLIFVDDLAHAARAWLDAPATPTATWSLHDGHDGGY
                     DWTLLCAIVGAACGREIRAWEIPRAPLELIARGNRALGRLLGRSPMLTPEKLRELRHP
                     DWVCDNNAITAALRWTPRTQFAEGLRLTPGWHD"
     gene            15022..15204
                     /locus_tag="PLW13_06685"
     CDS             15022..15204
                     /locus_tag="PLW13_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HQY70095.1"
                     /translation="MTYCRAGEPNPFNFRSDRFCQINGQWFFVTREKTQEGPYPSRDQ
                     ANAGAERYIARMQGRD"
     gene            15287..16078
                     /locus_tag="PLW13_06690"
     CDS             15287..16078
                     /locus_tag="PLW13_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY70096.1"
                     /translation="MKTPPDLPTDRYQQRKSRFGRCLTVYGRKTVLEALQAEGVACER
                     LHLAKSNKPAPLLEEIEALARAQGAEIVRHERAELARISRHAREDQGVAADVRTPGYR
                     QLAELMPLAPEPGAALLAVDGVTNPQNLGMLIRSAAAGGCQGIVLPRRGGCDICPLVI
                     KSSAGTVFHAPLLRCDSLPEALAQLRRGGWKISILDGHARLSLFDRLDERARVFVLGG
                     ETAGVSPAVRELADENLRIPMANGVESLNVAVTAALVAFRARLGR"
     gene            complement(16090..17226)
                     /locus_tag="PLW13_06695"
     CDS             complement(16090..17226)
                     /locus_tag="PLW13_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY70097.1"
                     /translation="MPVSIREYLDLLGALRQRLAFADIDAFYYLSRAVLVKDEKYFDK
                     FDRAFAAYFKDIETFDDLLDALIPDEWLRKEFEKFLSEEEKAKIQSLGGLDKLIEEFR
                     KRLAEQKERHQGGNKWIGTGGTSPFGSGGYNPEGIRVGPEGGQGRAVKVWEKREFKNL
                     DDSVELGTRNIKLALRRLRKFARSGAADELDMPDTIRSTAKNAGLLELKMVPERHNTV
                     KVLLFFDVGGSMDPHVRTCEQLFSAARTEFKHMEYFYFHNFIYDSVWRNNMRRQSEKI
                     PTIDILHKYPHDYKVIFVGDASMSPYEILQPGGSIEHWNEESGEQWMRRVAETYAKLV
                     WLNPVAEAHWEFSQSVDITRRLVEDRMFPMTLKGMEDAMGYLSR"
BASE COUNT         2689 a         6103 c         5734 g         2737 t
ORIGIN      
        1 tccaacctga acggaggacc ggaccatgca gaactgcgtt ttcatccaga ccaatcacaa
       61 gcagatcacc ggcgcgatcg tggcgcaaca cgcgctgcgc cgttactcga cccataacga
      121 ccagttcgat gtgaagatca tcgagaagaa agactacccc tacttcgacg cgcgcgaggg
      181 ccagacctac ctgcgcgacg gcgtcaagcg cgtgtggctg aacgacgacc tgcagtcctt
      241 cacgctgacg cgtttcatgc cgcccgaact gatgggattc cagggccgcg ccgtcgtgat
      301 cgacccggac gtgttcgcct gcgcggacgt gtgggaactg ttctcgcgcg acatgcaagg
      361 caaggccgtg atgtgccgcc cgcgctcggg caccaagggc ctgatcgaca agtgcctcgc
      421 caccagcgtg atgctgctcg actgcgcgaa actgcagcac tggaaggtgc aagagcgctt
      481 caaccggatg ttcgacttca ccttcgacta catggactgg atctgcctga agagcgaacc
      541 ggaagggacg gtcggcctgc tcgagcacga gtggaacgac ttcgacaagc tcacgcccgc
      601 cacgaagatg ctgcacacca cgcgccgtcg cacccagccg tggaagaccg gcttgccggt
      661 ggacttccgc atcgccgagc gcttccgcct gttcccgccg gcggcatggc tgatgaaggc
      721 gcgccgtcac ctgttcggcg agtacggcct gatggggcac taccgcaagc accccgacat
      781 caaccagcag aacttcttct tcggcctact caaggaatgc ctcgacaacg gcatggtgac
      841 cgagagcttc ctgcgcgagg aaatgcgcct gaaccacgtg cgccacgatg ccatcgaggt
      901 gctgaagagc acgcccgacc tgccgccggc gccgcgccac ccgctctccg gcgtcgcgca
      961 gaaggccgcc tgagggcgcg gcggcagttc aggcgccgtt cgccctgaac agcgccgcga
     1021 cggccgccgc agcgcgttct gcctcatcga ccgggtcgcg cgcgctcatc gccgccttgc
     1081 cctcgcccag ccagctcgcg cgcccgtccg ccactaggcg cccgattatc cgtccggtgt
     1141 cgcgacgcat gcgacgcggc ccgatggcca tcgccagccg gtgcgccagg gcgttgaagc
     1201 ggtagttgta gcggcgcttc caccagtgcg gaccgtcttc gaggctgaac aggaacagcg
     1261 gcttcgcggc aaacaacgcc tcggcgatca tcgacatgct gtcggccgtg accacgaact
     1321 gctccgcaag gccgagatag cccaggtagg gattgtcctc gcgcgctgcg ccccaacgat
     1381 ggcaatgcac ggcgacgtcg agctcggcga ggaacgcatc ggtcacgccg ggcggcgtgc
     1441 gcgcgctgtc tgtcaccagc accgaaccgt gctgcgaacg cgccagtccg ttgaccagcc
     1501 gcccgagccg gcgcgccttc tccggcgtga agacataggc gccgctgttg cccccgagca
     1561 acaccgcaat gcgcggcacc gcgagcggcg caaggcgcgc gcgccagcgt tgcgcctcgg
     1621 tcgcgagccg ctcccggcgc aggcgatgca tcggcagctc gttctcgagc acgttggggc
     1681 ggagcggcag gctgtactgg ggcgtggtga cgatcaggtc gaatacatcc agcggcgccc
     1741 acggcctgcc gacgtgcacg agccgcacgc ccggcgcctg tctgcggatc caccggccca
     1801 ccggctcgtt gcgccgcccg gaactgatca ccagatccgg ccacggcggc tgcagcgcgc
     1861 tcgaggcgcc gcgatcgatg ccgcacagcg tcacgccgag cagccggttg gccagcagtt
     1921 cccacggtcg gtagacgatt cgcttctcct cccacggcca cgcgagtgct tcggccagcg
     1981 ccagcacctg gttgttgtcg cccgccttgt ggccgagcag cagccagacg cgcggcgtca
     2041 ggctcgaatg cgcgttgtct tcgttcaccc cgggatattc ccttccttcg tcgtgccagc
     2101 cccgatccgc tgcattacaa tagcaacgct gcccgaggcg cgcgaccgcc gaccccgtcg
     2161 agcctctcct gctcgcgcaa gccggtgctg cggcatcatc cacggactgt gaccacggac
     2221 atcagccacc cgcatgctta tcgagcgcta catcacctcg gagatcgtcc gccccttcgc
     2281 ggccggcctg gcgattctgg cggccgtgtt catcgcgttc agcgcggcgg tgaagctctc
     2341 cgaagcggcc tctggcgaga tcgccgcgca cgtcgtggcc caactcatct tcctgaacac
     2401 gctgatcgcg atggaagtac tggtccccac cacgctgttc ctggccatcc tgttcgcgat
     2461 cgggcgcctc taccgtgact ccgagatggc cgcgctcagc gcggccggcg tcagcgaggc
     2521 cagcatcctg cgcgcggtcg gcaagctggg cctgttgtgc gccatcgcgg tggcgctgct
     2581 gtcggtattc gcgcgaccct gggcctacgc gaggacctac gcgctggagc agcagaccat
     2641 cagccgcttc gacctctcga acatccgcgc cggcacgttc accgacgtcg gcaacggcgg
     2701 ctacgtgctc tatgcgcgcg aggtcgacgt cgaagccgac gagatgcgcg gcgtgttcct
     2761 gcaggtcgag cgcgagcagc gctcgcaggt catctcggcc gagcgcgcgc gcatccacgc
     2821 catggacgag gagggctcgc gctcggtgga attcttcgac ggccacgcct acctgctcga
     2881 gcgcggtggc acgcgcgaca tcgacatgcg cttcaacagc ttcctggtcc gcctgcccga
     2941 ggaggagcgc atcagcaagt tccggcgcaa ggccgtggcc accggcacgc tgggtgactc
     3001 cgatcagccc aaggacattg ccgagtacca atggcggctg accacgccgc tggcgacgct
     3061 ggtgctggcg ctgctggccg tgccgctcag ccgcgccagc ccgcgccagt cgcgcaccgg
     3121 catcttcgcg gtcgccgtcg ccgtctacct gctggtgttc tccgtctcgg gcgtcgtgcg
     3181 caacgcgctc gagaacggcg acatcccgcc gttcccgggc ttgctgctcg cgtatgtgcc
     3241 gccggtcgcg ctgctggtgc tgctgatgct gatgccggga ctgcggcgac ggggatggcg
     3301 ccgatgatca tccgccgctt tctggccggg cagctcgcgc gcggctggtt catcgtgttc
     3361 ctcgtgctga ccgcgctgtt cggcctgctc gcgctggtcg acgagatcga caacctgagc
     3421 gagcgctacc gcctcgcgca cgcgctgcat tacgtgttgc tgaccacgcc acagcgcgtc
     3481 ctcgagctct cgcccgtgat cgcggcgctc ggtaccatcc tcgcgttcgc caccctcacg
     3541 cgcaacagcg agctcgtcgt gatccgtggc gccggattct cgctgcgcca actgctgcag
     3601 atctgcgcgg tccccacgct cgccatgatg ctcgcgctgg gttttgccgg ggaattcctc
     3661 gttgccgaca tgcaccagcg agccgaaacc gagcgcatgg tacggcgcag cggcaacctc
     3721 gacctgctcg acggccaggg gctgtggtcg aagagcggca accgcttctt caacgtgcgc
     3781 aacctgcgcg ccggacacat cccggagcag atctctctgt acgagttcgg accggacaat
     3841 gaactgcttc gcgcgatcga cgcgcgcggc gcggagctcg tcggcgagcg cgagtggaag
     3901 ctgatcgacg tgcagttcaa ggaatggcag gacggcaggg tcagcacccg gaacctgccc
     3961 tcgctggagc tcgggccgtt ctggtccgcc gcggagctgc cggcgctggg gcagtcgctg
     4021 gcagcgatgt cgccgcgcgc gctctacgcc tatgccaacc acctggagca aagcggcgcg
     4081 gacgacggcc aggtgcgcat ggccttctgg cagaaggcca tgctgccgtt ttcctttgcc
     4141 gcgatggtat tgctgtccgt cgttatcggc gtgggcttcg gcaccacgcg cagcgccgcc
     4201 ttcggctggc gcgtgctgtc cggcgccgtg atcggtgtcg gcttctatct tttgacccag
     4261 atattccata ccggcggaca gttgctgggc ctggggcagt ccgtcgtggt gctgatcccg
     4321 atctgcctgg tggtgctggc ggccgccgtc gttgcccgga tcacgcgcgg cccgcgctga
     4381 tccgcccgag gattcacgat gcgactgatg ctgagcttct accgcgccta tccctggcag
     4441 acgttcttca cgctgctggc gctttcggtg gcgggactcg ccgagggcgt cgggctctcg
     4501 gtgctgctgc cgctgctgaa catggcgatg gagggacagg ggcaggccgc gcccgccagc
     4561 gacaacgagt tctcgcagtc cgtgcgcggc gcgctgggcg tactgggcat cgcgccaagt
     4621 ctcggcaaca tgctgctcat cgtggtgttc gcgatcggct gcaagagcct gctgctgctg
     4681 ttcgccaagc gccaggtcgg gtacaccgcg gctcgcctgg caacggatct tcgcctcgag
     4741 ctgctgcgcg cgatcatgcg cagccgctgg gagtattttc tcggccagcc cgtgggcaag
     4801 ctgaccaacg cgctcgccac cgaggccgcg cgcgcctccg aatccttcgt caacggcgcc
     4861 accgccctca ccttcctcgc ccaggcggtg gtctacggcg ccgtggcgat cgcggtgtcg
     4921 tggcgggcga ctctcaccgg catcgtcgcc ggtgccgtga tcatcggcct ttcgcactcg
     4981 ctggtgcgca tcaccaagaa ggcgggccgc aagcagacct cggtgctgct gtcactgatg
     5041 gccacgatga ccgacacgct gggctcggtg aagccgctga agggcatggg ccgcgagcat
     5101 ctcgcggacg cggtgctgtc gatggagacc acgcgactga accgcgccct ggagcgccag
     5161 gcgctcggca gcgcactgct ggaatcgacg caggaattcc tgttcgcgct gatcatcgcc
     5221 ggcggcatct acgtggcgct ggcgaaattc gcgatgccct tcgccacggt gatggtgctg
     5281 gtggtggtgg tcgggcagat ggtggcgctt ctgggcaagg tgcagaagca gtaccagaag
     5341 atgacgctga gcgagagcgc ctactggtcg atgcagcgca ccatcgccga ggcgcaggcc
     5401 gaggaagagg agctgcgcgg cggcgagcgt cccgtgctcg ccacgcggct gtgcatggaa
     5461 ggggtctgct tcgcctacga cgagcggcgc gtgctcgacg acctcagcct cgagatcccg
     5521 gccggcgagc tggtgaccct gatcggtccc tcgggcgccg gaaagaccac ggtagtggac
     5581 ctgctgatcg gcctgatccg gcccgacgcg ggccgcatca cgctggacgg cacccccctc
     5641 gattcgatgg acatcaagca gtggcggcgc tccatcggct acgtgcccca ggaaaccctg
     5701 ctgttgcacg agagcatcgc gcacaacgtg agtctcggcg acagcgagct gtcgcaagcc
     5761 gatatcgagc acgcgctgcg cgccgctggt gcctgggact tcgtgagcgc gctgcccgag
     5821 ggcatgcaca gcaacgtcgg cgagcgcggc gggcggctct cgggcggaca gcgccagcgt
     5881 atcgtgatcg cgcgcgcgct cgtgcgccgt ccccagttgc tgatactcga cgaagccacc
     5941 agcgccctcg acgcggagag cgccgaagcc gtgcgccaga cgctggagcg cctgcgcggg
     6001 tcgctgacca tcctcaccat cacccacgag tccggcctgg tcgacatcgc cgatcgcgtg
     6061 taccggctcg agagcggccg cgccgtgcgg caatccccct aagccagcaa cgccttcacg
     6121 cgcgccaccg cgcgctcgat gtcagcgccg gccgcgccgg ggcgcggctc gcccggctcg
     6181 cccagccagg cggcgcgccc gtcgcgcagc agttgctcgt gcaccaggcc gatgtcgcga
     6241 ccgagccggc gcggaccgaa gcgcatcagc agggcatagc ccagcgaacg caggctgcgg
     6301 tcggcagcgc cctgcggtgc gcccgcgccg tgctcgcgcg cgagatcgaa catgtagacg
     6361 ggacgcccgg tggcgcaggc ctcggacagc atcgagatgc tgtcgaaggt gacgatcagg
     6421 cgctcggcgc aggcgagata cgcgaagtag ggattctgcg catccccggc cttccattcg
     6481 tagagctcgt gcgccgcgct gatgctggcg cgcagtgcgg cgagcgccgc gcgcggcgtg
     6541 cgcgcgctgg tggtgatcat caggctgccg ccgagcgcct cggccagccg cgatgcctgg
     6601 cgcccgagcc gcgccccggc gcgcggcccg aaggcatagg gcccgctggg gccgcccacg
     6661 atcaccgcaa tgcgcggcga gggcaacgcc gcgagcctcg cgccgtgctg ctcgcgcgcc
     6721 gccgcgaggc gctgcggcgt gacgcgatgc agcgtcagcg tgttctgcag cacgcgcgga
     6781 tcctcgggca agcggtactg cggcgtggtg atcacgaggt cgaaattgtg cgcatccgcc
     6841 cacggccgcc cgatgtgcac gatgcgcgtc atgccgcccg atgccctgcg gatccagcgg
     6901 cacacgggct cgttgcgcat gccggcggcg atcaccacgt ccggccacgg cgcgacgagc
     6961 gccgaggacg ccgccacgtc gatgcccata agcccggtac cgcgcacgag gttgaacagc
     7021 gcgccctgcc agcgatggcg cagctgcttg acctcgaacg gccagccgag cgcctcggcg
     7081 agcgcgagga gctggctctg ctcgccggcg cggtaggcgg cgatcaccca cacgcgcggc
     7141 ggtgtattcg tgttcggttc gggcacgggt ctgaaaatca ccaacattag ggagaatgcg
     7201 ggaggatgcg tcccgcggcc ggctcagtat aatccggggc gaaggcgcaa ctgggcgcat
     7261 cgactcggga aaacgcacga tggcacgtta cgaaccctgc ctggcgcggg tgctggaaat
     7321 tctcggcgcc ctcggcaagg ccaagaccac gatcaccgag gccagcgacc tcgctggcga
     7381 gctcggcctg tcctcgatcg atgtgatgga agtcatcgag caggtcgagg acgagttcga
     7441 catctcgttt ccgctgaact cgctgtcagg catccatacc gtgagcgacc tggcgcggga
     7501 aatcacgagg ttgtccacgt catgaaactc ctcgataaat tccaggcgat ggccggcgtg
     7561 cgcgccgagc tcgacgggct cggcatccgg cccttcggcg ccgtcaccga gaagatcctc
     7621 tcgcccaccg aggcgatcgt cgacgggcgt cgcgtgatcc tcgcgggcac caacaactac
     7681 ctcggcctca ccttcgatcc ggagtgcatc gcggcggccc agcgcgcgct ggccgaacag
     7741 ggcaccggca ccaccgggtc gcgcatggcc aacggcacct tcgccgagca cgttgcgctg
     7801 gagcgcgagc tcgcggcctt ctatggccgg cgccacgcaa tcgtgttctc gaccggcttc
     7861 agcgcgacca tgggcatggg cgcgacgctc gccggcagcg gcgacgtggt gatgctcgac
     7921 gccgacagcc acgccagcat ctacgacggc gtgcgcctcg gcggcgccga gatcatccgc
     7981 tttcgccaca acgatgtcga aaacctcgag cgcaagctgg cacgcctcgg cgagcgggcc
     8041 gcgaacacgc tggtgatcct cgagggcctc tacagcatgc tgggcgacat cgcgccgctg
     8101 gcggaaattg cgcagttgaa gaagcagtac gatttctgcc tgatggtcga cgaggcgcat
     8161 tcgctgggtg tgctgggcga gcgcggccgc ggtctcgccg aggccgctgg cgtggaggag
     8221 gccgtggact tcgtggtcgg caccttcagc aagagtctcg gttccatcgg cggctactgc
     8281 gtgagcaacc atgcgcagct ggaagcgatc cgcttctcga tccgttccta catcttcact
     8341 gcctcgccct ccccggccgt gatcgcctcg acgcgcgtgg cgctgcgcct catgcaggag
     8401 cgcccgcagc tgcgcacgcg gctgtggcac aatgccgagc ggctctatgc gggactgcaa
     8461 gggctcggct tcacgctggg accgcaagcc agccccgtgg tcgcggtgcg gatcgaggac
     8521 cgcgagaccg cgctgcgctg gtggccgcag ctgctggagc agggcattta cgtgaacctc
     8581 gtggtgccgc cggcctcgcc ctctgcgttc agcctgctgc gctgcagcgt cagcgccgcg
     8641 cacagcgacg accaggtcga tcgcatcatc gctgccttcg ccacgctgcg gaccggcgcg
     8701 tgagcgaggc cgccgcggac gccccggcgt tccgcttcgc gcagatctcc gacccgcacc
     8761 tctcggatct ctccggcgtg cgctggagcc agctggcgag caagcgcgtg ctcggctacc
     8821 tgtcgtggcg gcgccggcgc cgccacgaac accggcccga gatgctcgct gcgctgagtg
     8881 ccgacctgca cgcgatcgcg cccggtcaca cggtcgtcag cggcgatctc acgcacatcg
     8941 ccctgcccgg ggaattccgc gaggcgagcg actggctgcg ccagctggga ccggccgagg
     9001 cggtgaccgt ggttccgggc aatcacgacc gctatgtgcg cacgccctgg gcggagtcgc
     9061 tcgggctgtg gagcagctac atgagctcgg atgacggcag cgcggagcac ggggcggagc
     9121 agctgtttcc gctgctgcgg gtgcgcggac cgctggcatt catcggtctc gacagcgcgg
     9181 tgccctcgcc tccgttcatg gcgaccggcc gtctcggcaa gcgccagatc gagcgtctcg
     9241 cggctatcct cgaacgcctg cgcggcagcg gactgctgcg cgtgctggtg ctgcaccatc
     9301 cgcccgtgcc gggcgaggag aaatggcgca agcgcctggt ggatgcgccc gcgctgtgcg
     9361 cgctgctcgc gcgtcatccc gtggacatgg tgctgcacgg ccatcgtcac cgcgcgctcc
     9421 agagcgtgat ccgcatcccg ggcagcgaga tcccggtatt cggcattccc tcggcttccg
     9481 ccgccggcgt gctgtcgaat taccccgcgg aatacaatct ctacgacatc atccgcgacg
     9541 gcgcgtcatg gcgggtgcgg gtcagcgcgc gacgcttcga cgccgccacg cgcgccttcg
     9601 ccggccgcga gctcgcgagc ttcgcgctgc gcaacgacgc gtcgcgctga gtcccgcgat
     9661 caggccgcgc cgcgcagcgc gcgcaggcgc tgctgcacca gttcgtgatc cgacagcgaa
     9721 tcgacgtcca ccgccgcgtc accgaagggc atcctcaccg cagccagcct gagccccatg
     9781 cgccgcgaca gcgcgcgctc ggcgcccgcc agcgacaacc agccgaagcg gtagcgcagc
     9841 accgagagcc agcccagcat tttcatcacc ttgagcggcg acttgcgctc ggcctcgacc
     9901 gcgcgccagc gttcggccat ctgccggccg gccggcgtga ggaacgcgaa caggttgcag
     9961 ccgcagtact cgtcgtcgct gaagcgcagc accgtcttgc gcatggccgg gaacagctcg
    10021 cgcaccaggg cgtagggcgc gagaccgacc acggcgtcgg cccccgtagc gagcgcctcg
    10081 cggcagaagt gatcgatgat gtcgctgcgc agcagcggat ggtccgcggt ggtgagcagc
    10141 accggcgtgg cggacggcgt cgccgccagt gcctgccagg cgctggtgct gggcgtggcc
    10201 tgcggcggtt gccagccgat gcgctgctcc gcgatcaggt gctgcaggcg cgcctcggtc
    10261 gcgatgcttt cccgcgacgg accgcagatc gtgatctccc ccacctcagc cgaatgcccg
    10321 agcgcatcca gcacccgcag gatcatcggc gtgccgtcga tctccaccag cgccttgcag
    10381 ccacgcccgg acgccgcgac cagcgggtcg ctgctcgaac ggtcgcccgc gagcaccagc
    10441 gcggcgaatg gcctgttcct gtcgttcccg ttcatgggct atatcgcctt ctcgtagatg
    10501 cggtagcgct tgtacacctg gccgccgatg gcctcgatga tgctgcgcat gccggaattg
    10561 tcctcgagta tccacgacag ctcgacttcg cgcacgccgc gcgcgcacaa gcccttgcgc
    10621 accgcgtcga tcaccatgaa ggccaggccc ggaccgagcc gcgactgctg gaacttcttg
    10681 cgcacgccca tcaggggcac ccgcgcgctc tgcgggtgac gcaccttcag cccccacagc
    10741 aggcgcagcc agcccagcgg cagcaggcgc ccgcgcagcc cggcggtcac ttcgtttatg
    10801 ttcggcatcg ccacgatgaa cgcggccggc tcgccgtcga cctcggcgat ctgcacgtaa
    10861 tcgtccggca gcagcagcgt catcagcttg ccgacgtcgt tgaactcctc caccgtgaac
    10921 ggcacgaagc cgtagttctg cgaccaggcg tcgttgaaga tgtcgcgcag gatccccagc
    10981 tcgtgcgcct tgtcgcggcg gatcatcggg cgcacgcgca cctggccgga aagcgactcc
    11041 gcgagccggc gcatgacccg cggcgcctcg aaatcgggcg cgacgcggta agcgagcagg
    11101 tcgcgcgccg gcgccagccc gcagcctcgc agcagggcgt cgtagtacgg acgcgcgtgc
    11161 cccatcatga tcgagggcgg tgtgtcgaaa ccctccacca gcagcccgca ctcctcgttg
    11221 accgagaggt tcagcggccc gcgcacgcga cgggcgccgt gcgcgcgcag ccacgcgctc
    11281 gccgtgtcca gcagtgccgc gaccaccgcg gggtcgtcct cggcctcgat caggccgaag
    11341 gatccgctca tgtcgtcgtg gtattcctgc tgcagccggt cgaactgggc gctgatccgg
    11401 cccaccgccc gcccctgcgc atacgccacc caggcctgcg cgctcgcatg ctgcaggaag
    11461 ggattccccc cccacaggcg ctcgcgcaac tccacttcca gcggcgcaac ccagtgcggg
    11521 tcgttcaggt acagctgatg cggcaggcgc aggaactcgc gcctgaggcc ggcggagtcg
    11581 gccgcctgca cggtgatcgg gggatgcatg gacatgtgct gctggcgcct ccgggaccct
    11641 gcccgcggga atcgccgcgc agtatacagg ccgctcctgt gagcggcccg cgcaacgtca
    11701 ctattcacct gtgcggcgcg tcggcctcgt ggattttctt cattgaaatc cgataattag
    11761 ctgctaaggt ggaagctcga aacgggcctt tcgcggccaa atagtaccac tcgatgcagg
    11821 ttgatttggc ggcgatccca acgaagaata agagggtggt caacggctcc agatgagtcc
    11881 cgccaatgcg tggcggacgg cggggacggg gagtagcgcg aggtcgtatt ccggagcatc
    11941 cggggcggcg gccttcgtga tggcaaacaa caagggaaag gtaacgtttt gcacaccgaa
    12001 tccacaccta ccagacattc cctgccgttc cgcggggctg acttcacgac gctggcggag
    12061 gcactcgact acgccgcaga cggggcgacc ggaaccaact tctacagcgg tcgcggccag
    12121 ttgtacgcca cgctgtccta tacggatctg cgggcgcagt gcctggtgct ggcgcgcaag
    12181 ttcacggggc tcggcctggt aaagggtgac cgggttgcga tggtggcgga aacgcacccc
    12241 gatttcctgc gcgtgttctt cgcctgccag tatgccgggc tgattcctgt cgccctgccc
    12301 gcgaccatca atctcggtgg acaccagagc ttcgtctcgc agctgcgcgg cctgatcgag
    12361 aacagcggcg cggcagtcac gatcgcctcg gaggccttca tgcccttcgt ggtcgaggcg
    12421 accgagggcc tcgaactgcg catgatcggc gaaccggcgg aattcgacgc cctgcccgag
    12481 agccccgacg cgttgccggc catcgggccc gacgatgtct cgtacctgca atacacctcg
    12541 ggcagcaccc gcttcccgcg cggcgtgatc atcacgcacc gctcggtgat gagcaacctg
    12601 tcgggcatca tcgcgcacgg cgtgaaaatc ggcgaaggcg accgctgctt ctcgtggctg
    12661 ccgttctacc acgacatggg cctggtcggc ctggttctgg tgcccgtggc ctcgcagatg
    12721 tcggtcgact acatggacac gcgcgatttc gcgatgcgcc cgcgccagtg gctggccctg
    12781 atgtcgcaga ccaaggcgac gatatccttc agcccctcct tcggttacga gctctgcgcg
    12841 cgacgcctgc gcgagggcga agccgcgcgc tacgacctca gcaactggcg cgtcgccggc
    12901 atcggcgccg agatgatccg ctgggagacg ctcgaccgct tcgccgaggc gctggcgccc
    12961 gcgggcttcg acgcgcgcgc cttcctcgcc tgctacggca tggcggaatg ctcgctcgcc
    13021 gtcagcttcg cgccgctgtt caccggctac cagaccgatc gcatcgatct cgacgaactg
    13081 gccgacaacg gcaaggccct gccggtcgcg cagggcgacg aatcgaggcg tacgcgcgcg
    13141 ttcgtgaaat gcggcgaacc gctgccggaa tacgagatgg aaatccgcga cgagcgcggt
    13201 gccctgctgc ccgatcggca caccggcacg atattcgtgc gcggccccag cgtgatgtcg
    13261 ggctacttca acgccccgca agacattggc ggagcgctca gcgcggacgg ctggctcaac
    13321 acgggcgaca tcggctactc ggtggagggc agcctcgtca tcaccggccg ccagaaagac
    13381 ctgatcatca tcaacggccg caacatctgg ccgcaggatc tggaatacct cgccgagcac
    13441 caggacgaga cccgcgtcgg cgacgcgctc gcgttctcgg tgccaggccc ggagagccgc
    13501 gaggtctgcg tgctggtggt gcaatgccgc gagacgaatg ccaaccgccg cgccgcgctg
    13561 atcgaccgcc tgacgcgact ggtgcgtatc gagatgggca tcgactgcta cgtcgaactg
    13621 gtgccgccgc acaccctgcc gcgcacctcc tcgggcaagc tttcgcgctc caaggcccgg
    13681 cagaacttca tggattcgca cgacctggcc gaagtggccg ccatcgccga aggcatgtcg
    13741 ctgaaacagg cctcggcctg agcccgcagc gcgcaccggc acggccctga ggtgcgcgtc
    13801 gcgatcaccg gggcgaccgg gttcatcggt cgccgcatcg cctccctgct ggaatcgccg
    13861 caatacctcg tgcgcgcgct cgcgcgcgac gcggcctcgg cgcgtcacct gctcgcaccc
    13921 ggcgtggaaa tcgtgcccgg agacctcgcc acgccgcagg cgctgcgcgc actggtgcgc
    13981 gacgccgacg ccgtgctgca tctggccggc gccgtgcgcg gtgctacccg cgcggacttc
    14041 gacgccgcca acgtcgatgg cagcgccgcg ctgctcgccg cgatggacgc cgccgcgcca
    14101 cacgcccgcc tgctcttctt ctcgtcgctc gcagcgcgcg agcccgcgct ctcgcattac
    14161 gccggcagca agcagcgcgc ggagcaattg ctgctgaggc gcgccgccgc gcgtcccgtc
    14221 acgatcctgc gcccaccggc ggtctacggt ccgggcgatc gcgagatgct gccggtgttc
    14281 cgcttcatgg cccgcacggg gcgcgcgccc tgcgcgggca gggccgcgga ccgtttgtcg
    14341 ctgattttcg tcgacgacct cgcgcatgcc gcgcgagcct ggctggacgc ccccgcgacg
    14401 cccacggcaa cctggagcct gcacgacggc cacgacggcg gctatgactg gaccctgctg
    14461 tgcgcgatcg tgggcgcggc ctgcgggcgc gagatccgcg cctgggaaat cccgcgcgcg
    14521 ccgctggaac tgatcgcacg cggcaaccgc gcgctgggcc gcctgctcgg acgctcgccg
    14581 atgctgacgc cggaaaagct ccgcgaactg cgccaccccg actgggtctg cgacaacaac
    14641 gcgatcaccg ccgcactgcg ctggacaccc cgcacgcaat tcgccgaggg tttgcgcctg
    14701 acgcccggct ggcacgactg agcacgaccg gggttgccgc gccgcgccgc gaaggaccgc
    14761 catgcgttcc acggcagcgc gggatcgacg gcgcattgca caccggtccg ggtccggccc
    14821 gggcgttgtt tcccgatcgg cgcgcgcagt gggatgcagg gcgcatccgg cgtctatcga
    14881 cttgattttt gtcaactttt tttacattgc aggctgccgt cgacgcaccc tccgggagga
    14941 agcgtatgat tatcacgtcg ttttgttgcc gggcatgcaa cttgcttcac ccagtgaaaa
    15001 ccacccaacg acagaaaacc gatgacgtac tgccgggccg gcgagccaaa tccattcaac
    15061 ttccgctcgg accgcttctg ccagatcaac ggacaatggt tcttcgtcac ccgcgaaaag
    15121 acgcaggaag gaccctaccc gtcccgcgac caggccaatg ccggcgccga acgctacatc
    15181 gcgcgcatgc aggggcggga ctgatcgcga agcccccgct ccccggccgc gcgccggcgc
    15241 atcgagcgcc ggccatcgtg cgccctgcaa ccggcgcatc ccgcccatga aaacgccgcc
    15301 cgatctcccc acggaccgct accagcagcg caagtcccgc ttcgggcgct gcctcacggt
    15361 ctacggccgc aagaccgtcc tcgaagcgct gcaggccgaa ggtgttgcct gcgagcgact
    15421 gcacctggcg aagagcaaca agcccgcccc gctgctcgag gagatcgagg cgctcgcgcg
    15481 tgcgcaaggg gcggaaatcg tgcggcacga gcgcgcggag ctcgcccgca tctcgcgcca
    15541 cgcgcgcgag gaccaggggg tcgcggccga cgtgcgcacg ccgggctacc gccagctcgc
    15601 cgaactgatg ccgctggcgc ccgaaccggg cgccgcgctg ctcgcggtcg atggcgtcac
    15661 caacccgcag aacctcggca tgctgatccg ctcggcggcc gccggcggct gccagggcat
    15721 cgtgctgccg cgccgtggtg gctgcgacat ctgtcccctg gtgatcaagt cgagcgcggg
    15781 gacggtgttc cacgcgccgc tgctgcgctg cgacagcctg cccgaggcac tggcgcaact
    15841 gcgccgcggc ggctggaaga tcagcatcct cgacggccac gcgcgcctca gcctgttcga
    15901 tcgcctcgac gagagggcgc gggtgttcgt gctgggcggc gagaccgctg gcgtcagccc
    15961 cgcggtgcgg gaactcgccg acgagaacct gcgcatcccg atggccaacg gcgtcgaatc
    16021 gctgaacgtg gccgtgaccg ccgcgctggt ggccttccgc gcccggctcg ggcgctgaat
    16081 gcccggccgt cagcggctca ggtatcccat cgcgtcttcc atgcccttga gcgtcatcgg
    16141 gaacatgcgg tcctcgacca ggcgccgcgt gatgtcgacc gattgcgaga actcccagtg
    16201 cgcctcggcg accgggttca gccagaccag cttggcgtag gtctcggcga cgcgacgcat
    16261 ccactgctcg cccgattcct cgttccagtg ctcgatgctg ccgccgggct gaaggatctc
    16321 gtagggtgac atcgaggcat cgccgacgaa gatcaccttg tagtcgtgcg ggtacttgtg
    16381 gaggatgtcg atggtcggga tcttctcgct ctggcgccgc atgttgttgc gccacaccga
    16441 gtcgtagatg aaattgtgga agtagaaata ctccatgtgc ttgaactcgg tgcgcgcggc
    16501 cgagaacagc tgttcgcagg tgcgcacgtg cgggtccatg gagccgccga cgtcgaagaa
    16561 cagcagcacc ttcaccgtgt tgtgccgctc gggcaccatc ttcagctcga gcaggccggc
    16621 gttcttcgcg gtggagcgga tggtgtcggg catgtcgagt tcatcggcag cgccgctgcg
    16681 cgcgaacttg cgcagccgcc tgagcgcgag cttgatgttg cgcgtgccca gttcgacgga
    16741 atcatcgagg ttcttgaatt cgcgcttctc ccagaccttg accgcgcgtc cctggccacc
    16801 ctcgggaccg acgcggatgc cttccgggtt gtagcccccg gagccgaacg gcgaggtgcc
    16861 gccggtgccg atccacttgt tgccgccctg gtggcgctcc ttctgctcgg cgagacgctt
    16921 cctgaattcc tcgatcagct tgtcgaggcc gcccagcgac tggatcttcg ccttctcttc
    16981 ctcgctgagg aacttctcga attccttgcg cagccactcg tccgggatca gcgcgtcgag
    17041 gaggtcatcg aacgtctcga tgtccttgaa ataggccgcg aacgcacggt cgaacttgtc
    17101 gaagtacttc tcgtccttca ccagcaccgc gcgactcagg taatagaacg cgtcgatgtc
    17161 ggcaaatgcg aggcgctggc gcagcgcgcc cagcagatcg aggtattccc ggatcgagac
    17221 cggcacgccg ctctataaca tcgacttcgg gcccgaatca ctc
//
LOCUS       DAORUQ010000054        17221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3005_length_17221_cov_6.237854, whole genome shotgun sequence.
ACCESSION   DAORUQ010000054 DAORUQ010000000
VERSION     DAORUQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17221
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3005_length_17221_cov_6.237854"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..514)
                     /locus_tag="PLW13_06700"
     CDS             complement(83..514)
                     /locus_tag="PLW13_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70098.1"
                     /translation="MPDRPITELSPEQQDLWQRVNDLWSLSLERNAERIRSTLHPQYV
                     GWDMNQPITHDREAAIRAVLGDAPTVTGYELVPLSVQVYDHTVGIVHYMYSASVAPKD
                     AAPVRIAGKWSEVYLRQDCQWVMISVSGRPDPTTENSSAVA"
     gene            1695..2282
                     /locus_tag="PLW13_06705"
                     /pseudo
     CDS             1695..2282
                     /locus_tag="PLW13_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
     gene            complement(2241..3269)
                     /gene="moaA"
                     /locus_tag="PLW13_06710"
     CDS             complement(2241..3269)
                     /gene="moaA"
                     /locus_tag="PLW13_06710"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQY70099.1"
                     /translation="MNAIVPDAAAVQQAPALIDRFGRRVDYVRLSVTDRCDFRCVYCM
                     GEDMQFLPRREILSLEELLAAAEAFVALGVTKIRLTGGEPLVRQNIVWLAGRIAALPG
                     LRELVLTTNGSQLPRLAAPLREAGVKRINISLDSLDRARFRQLTRTGDLGTVLAGIDA
                     ALAAGFERLKLNSVILRGRNDDEVLALVEFARARDIDISFIEEMPLGHIEEHDRATSF
                     CSSAELRAIIETRYPLTPSLDSTGGPSRYWRMADSATRVGFISPHSHNFCHLCNRVRL
                     TVEGRLLLCLGNEHSADLRAVMRARPGDRTALREAIVQAMDLKPERHHFALDGEPQIV
                     RFMNTTGG"
     gene            complement(3266..5515)
                     /locus_tag="PLW13_06715"
     CDS             complement(3266..5515)
                     /locus_tag="PLW13_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY70100.1"
                     /translation="MEATAAPEGPPGQATETVFRSCPVCEASCGLAVAVDRAANRVVS
                     VRGDAEDHRSRGYVCAKSQVVKDLHEDPARLRRPVRRTENGWEEMEWEAALALVGERL
                     AAIRAAHGNNAVAMYYGNPNGHNFGAQIYTQLFISLLETQRFFSAGSVDQQPKNLSCE
                     LLYGSAWTFPIPDLDRSDFFVCMGANPLVSQGSLMSAPDAGGRFEALRARGGELVVID
                     PRRSETAAIADWHLFIRPGTDAFLLFALVNLLFASDRVDLGHLAGRIDGLERLRELAA
                     PFTPEAVAGITGVAPADVRRLCERFCAAERPVIYGRIGLCTQEFGTLASWLVDVVNLL
                     RGRLDAEGGAMFPRPATGQGEDSDSVGQLLRGRWRSKVRGFPEYMSMLPATLMAEELA
                     WDGPDRARALITVAGNPVLSVPNGDRLREAMDGLDFVVALDIYINETTSRADVILPST
                     VQLEHSNYDFLFQSTSVRNFARYSPRVLTPEPGARDQWQLMLEICGRVNGLTVEQLDA
                     MIFEGLAERVGARLPAVGAERVKELAGTEAGPERLLDMMLRAGPYGDGFEGGEGLTLA
                     RVKAAEHGIDLGPLTPGRLPGILRTEDKRIRLLHPLLEEDLGRLHERLRAGAPSGLLL
                     IGRRHIRDMNTWLHNLPHFVRGRNRCTLRMHPGDAAARGLSDGATALVRSAAAERAVP
                     VEISDEMMPGVVSLPHGFGHRHADTGQPHAQAHAGVSANDLIGDGLDLPSGTSIVNGV
                     PVEVRAAPS"
     gene            complement(5493..5732)
                     /locus_tag="PLW13_06720"
     CDS             complement(5493..5732)
                     /locus_tag="PLW13_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQY70101.1"
                     /translation="MSAPVRAGDAPIAVEVEAGKSYWWCSCGRSAKQPFCDGSHKDTG
                     FTPLKYDAPETRTAYFCACKATANKPLCDGSHSRT"
     gene            complement(5749..6228)
                     /locus_tag="PLW13_06725"
     CDS             complement(5749..6228)
                     /locus_tag="PLW13_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQY70102.1"
                     /translation="MTIKVGDRIPSVNLKTMGEKGPESISTDDIFNGKKVVLFSVPGA
                     FTPTCSAAHLPGYVVNADKIKAKGVDTIACMAVNDAFVMGAWGKAQNAEELLMLADGN
                     AEFAKALGLTLDGSGFGMGTRSQRFALVAENGVVKHLAVEPGPGLDVSAAEKILAVL"
     gene            complement(6325..7101)
                     /locus_tag="PLW13_06730"
     CDS             complement(6325..7101)
                     /locus_tag="PLW13_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70103.1"
                     /translation="MSAQDGGQKAKIRRIMLALDAMNCEAALMEAAALIAAKLEAELD
                     ALFVEDADVYAVADLPITHEISLGSGRERAITGSHVEQALRCLSREAEQRFSAVTRRS
                     RVKGEFQVTRARRGEALSEASARVELLLMQPRERTIVRVRIQETRPPRVFVLCGATPA
                     STRALELAARLAQQDHHVLEVIAAGEVDAAQLAEIGKTGLRVNLHPHPVNTDSLEMLR
                     EVENRLGNMVLIAGDLTPAKDRRAVLEELSHLRCQVLLVN"
     gene            complement(7098..9518)
                     /locus_tag="PLW13_06735"
     CDS             complement(7098..9518)
                     /locus_tag="PLW13_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY70104.1"
                     /translation="MAVETLKADALSRATDAQALGFRSTAELPDVDSAMGQDRAIAAI
                     EFGVGIQREGYNLYVAGPTGIGKRTLVDQLLARHAGERGEASDWCYVNNFEDPSRPRA
                     LALPPGKGVQLKRDMEKLIERMLVTVPNVFRGDEYRRRISDIQEEYQEREGKLFGELN
                     DEARGKGMVVIRTPGGYTIGPVREGKLMTPAQFNDMPKEEQELAKNVIEELNRRLKSI
                     IESIHDWQEESTERVKGIDNEFIHDIIDPMLAALKSRYQMYPSVLEFLGAAHQDMAEN
                     IWDFMPNEQQEGSTPLRKKIFAMEHLRYHVNVLVDHDGAADVPVLYEDNPTLQNVIGR
                     IEHIAQMGTLVTNFSLIKPGALHRANGGYLVLDARKVLMNSFVWDALKRAIRSREIRI
                     ESIERILSMVSTTSLEPEPIPLDVKIVLVGDRYIYHLLKEYDPEFSLYFKVNADFSDS
                     MDRSPEAVGLYARVIATLARREKARALSAGGVARVIDQCARDAEDSHKLSLHLGDLVD
                     LILQADFWAARENIALIEARHVDQAIAQRRFRNNRYQELLAEQIHRGVMLLATDGMQV
                     GQINGLSVIQLGDYAFGRPSRITATARLGAGKLIDIERETELGGPLHSKGVMILSSFL
                     ARRYAQSQPLALSASIVFEQSYGRVDGDSASMAELVALLSAIAGVPIRQDLAMTGSVN
                     QLGEMQAIGGVNEKIEGFFDVCAARGLSGTQGVVIPASNVQHLMLKPEVVEACRAGNF
                     HVHAASAVDDVIALLTGMPAGAMREDGSFETDSVNERVRARLHELAEIGRKHSGSGDD
                     KAAGNGKP"
     gene            complement(9535..9861)
                     /locus_tag="PLW13_06740"
     CDS             complement(9535..9861)
                     /locus_tag="PLW13_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQY70105.1"
                     /translation="MPDQQTLRARLIAKRDELEERVRRIDADLRKRDEPLSADFAEQV
                     VEQENLDALYAIESEGRLELQKVVHALARMDRGEYAVCSRCGGPIAPERLAALYYADT
                     CIGCAD"
     gene            complement(9934..10458)
                     /locus_tag="PLW13_06745"
     CDS             complement(9934..10458)
                     /locus_tag="PLW13_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY70106.1"
                     /translation="MTSSRIHTTLPGISLRQAERPDAALVLDFIHKLAAYEKLSHEVQ
                     ATVGDIEDALFGARPFAEAAIAELDAAPVGFILWFPNYSTFVGKPGIHIEDLFVLPHA
                     RGRGIGRCLLAWAARVAQERGWGRVEWSVLDWNEPALRLYQSIGAEAKTEWVLQRLAG
                     AALVRLAGQFPARD"
     gene            10463..11137
                     /locus_tag="PLW13_06750"
     CDS             10463..11137
                     /locus_tag="PLW13_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQY70107.1"
                     /translation="MDILETAGRPGRRHWLARRGTAGASLAAGRARRKRTLRALLVLL
                     CLGGCTDLRVTHQVQDPAADIGSQQAFGFLRAPLAADARASAEARTMDSAVRAAITGA
                     LQARGYTPALPGYKPALGIDYTLSDVVGANARRLDSPSDYSRSWRAGGAGDGTGSMDH
                     TIADAAFYRELTLTVLLFPSSTGALAWEGSARQSFPGDLPQGARLQAALDRMTRKLLA
                     QLPPAL"
     gene            11325..12197
                     /locus_tag="PLW13_06755"
     CDS             11325..12197
                     /locus_tag="PLW13_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70108.1"
                     /translation="MRFAYHASMPAPDQYLPLARAAEELGFDSFTFPDSICYPKESNA
                     KYPYNDDGSRDFLDGVPFLEVFIAMAAAAAVTTKIRITTSVVKLPIRQPAIVAKQLSS
                     LAVLSNNRIALGVGLSPWPEDFEACQIPWEKRGQRMDEMIEIIRGLMKGDYFGYKGQI
                     FEMEPIKLCPVPSQPVPILLGGHADAALKRAARIGDGWISAGTSLEELDGYLKKLHAF
                     RAEFGRDKEPFEVQNMGASAYTPDGIKQLEDRGVGEVIVAFRDVYKKQPDTSLEEKLA
                     TMRWYADNVIAKTR"
     assembly_gap    12201..12300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12345..13916)
                     /locus_tag="PLW13_06760"
     CDS             complement(12345..13916)
                     /locus_tag="PLW13_06760"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HQY70109.1"
                     /translation="METATISTSTLYTDLSSALLVEHAIRRKEGHLADTGALVVTTGR
                     RTGRSPADRFIVDEPSSNDAIDWGAINRKFDAEKFNALWDRVVEFSSEHDRYVSHLHV
                     GADLEYYIPIRVTTENAWQNLFARNMFIRPEKYNPRGKEEWQVLNIPSFLCDPERDGT
                     NSDGAVIINFAQRKVLLANMRYAGEMKKAMFSVQNFLLPERDVLPMHCAANVGHDGDV
                     ALFFGLSGTGKTTLSADPERYLIGDDEHGWAEGAVFNIEGGCYAKTINLSRKNEPLIW
                     DAIRFGAIVENVVTHEHHRIADYTDTTLTENGRCCYPREHIEKRAERIAAGEPRAVIF
                     LTCDVTGVLPPVSILSKEAAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPR
                     PAHVYADLLIRRIEKFGSQVYLVNTGWTGGSGGVGGAGFRFPIPTTRAIISAIQSGAL
                     AGAPTEHLPGLNLDVPTHVAGVEEHLLNPRNTWEDKAAYDAQMRNLIGQFQQNFRKFS
                     GVSESIIAAGPHMPE"
     gene            complement(14051..14926)
                     /gene="hslO"
                     /locus_tag="PLW13_06765"
     CDS             complement(14051..14926)
                     /gene="hslO"
                     /locus_tag="PLW13_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HQY70110.1"
                     /translation="MSPPDTDTLQRFHFETAAVRGELVRLGPSLREILGQHFYPGPVA
                     ALLGESLAASVLLSSTIKFAGSLSLQAKSEGPVSILFGECTHDRQVRGYARVADGAVG
                     GGLDELLSRGTLAITITPAEGQRYQGIVPLDTPRLAGCLEHYFTQSEQLPTSILLACD
                     GEHAAGLLLQALPGSATAVDDQAARWEHLHQLACTVSDAELLGEAFETLLFHLFHEER
                     VRVQAPEPVRFGCRCSSSRAGATLQSLGEPEARAILAEQGTILIHCEFCQQEYRFDAA
                     AIDAMFHVEVPAAKH"
     gene            complement(14923..15318)
                     /locus_tag="PLW13_06770"
     CDS             complement(14923..15318)
                     /locus_tag="PLW13_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQY70111.1"
                     /translation="MSEERVRLDKWLWAARFFRTRNLAKQAIEGGKVWYDGARTKVSK
                     EVQVGAELRIRQGFDEKTVTVRALSEERRGAPEAALLYEETAASLAARTAGADERRLQ
                     RAGDILHEGRPSKRDRRLIHQFKERHSDS"
     gene            complement(15311..15982)
                     /gene="yrfG"
                     /locus_tag="PLW13_06775"
     CDS             complement(15311..15982)
                     /gene="yrfG"
                     /locus_tag="PLW13_06775"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HQY70112.1"
                     /translation="MVDWNDIETVLLDMDGTLLDLHFDNFFWLEHLPRRYAEIFDRKQ
                     EEARLELVERFRAEQGNLNWYCIDYWSRELGLDVRAMKEEMRHLIAVRPHVEDFLARL
                     HASRRRVLLVTNAHHKSLSLKLEQTGLERWFDGIICSHDFNLAKEQPGFWAALASVEP
                     FEREHTLLIDDNHAVLQSAHEYGIRYLLTLLQPDSRREARAGLYFPAILHFDEIMPEL
                     DDVDV"
     gene            16115..16675
                     /gene="nudE"
                     /locus_tag="PLW13_06780"
     CDS             16115..16675
                     /gene="nudE"
                     /locus_tag="PLW13_06780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HQY70113.1"
                     /translation="MSDHPGPEILASRWVSHSRLFHVEELRLRFGNGVERTYERLNPG
                     FHGAVMVVPVRDDGRLLLVREYGAGVGDYYLSFPKGAVHAEESMLDAANRELMEEVGF
                     GAHELELLTELSLSPSYMGNRMSIVLARGLFEKRLPGDEPEPLTVSVHTFDELIELQA
                     SGVLNEAYAVAALFLARERLAAAEAP"
     gene            16708..>17221
                     /locus_tag="PLW13_06785"
     CDS             16708..>17221
                     /locus_tag="PLW13_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQY70114.1"
                     /translation="MAAAREAGAAILPLRRAGSDADRKPDGSLVSAADRAASAIIGAR
                     LHALDPALPVICEEAAQDTAGAARFWLVDPLDGTREFLRGSDVFSVNIALIEDGYPCF
                     GAIHLPASDLDYWGACGAGAWRDGCAIRARRLAEAGAGLHALVSPGERDSAPREFAAT
                     LARHGGTQRIE"
BASE COUNT         2718 a         5778 c         5716 g         2909 t
ORIGIN      
        1 taacgcataa cgtagaactg agcggcctgc gcggctcttt gcgcaggtcc gctcgactgc
       61 cgggttgggc ctcggcggcc gactatgcaa ccgcactgga attttctgtc gtcggatcag
      121 gtctcccact gacgctgatc attacccatt ggcagtcctg gcgcagatag acctcagacc
      181 acttgccggc aattcggact ggcgcggcgt cctttggagc gactgatgcc gaatacatgt
      241 agtgcacaat cccgacggta tggtcgtaaa cctgcacgct cagcgggaca agctcgtagc
      301 cagtaacggt cggcgcatca ccgagtacgg cccggatagc ggcctctcgg tcgtgggtta
      361 tcggttgatt catgtcccag ccgacatact gaggatgaag ggtcgatcga attctttcgg
      421 cgttgcgctc cagcgacaaa gaccacagat cgttcacccg ctgccacaag tcctgctgct
      481 cgggagaaag ttctgtaatc ggacgatcag gcatggcgcg tcattcttga tgatgcataa
      541 cacttgagcc caggccgcgg cccgtcaggg ccgtcgtgtc tgcagcgaat tgttatgtgc
      601 catacgcaag gctcgccgac actgcgagga agaacgagcc tagtacatac agcagctgat
      661 aaacgaaaag gtccacttgc tctggcggcg tgtttccttc aagcgtctgc atcggccacc
      721 cgcctctgcc cagcgttgcc caaagaagta cgccgatacc caagtattgc gaatactccg
      781 agagtgactt tgaacactcc accccaatag cccccagaag tattccggcg caccagccag
      841 tggaaactgc cgccaacacc catgcgagag acttgatcaa tccagatcta atttcccgag
      901 cctgctttgc cgaatgcgat ccggcgttac gagccgaact cgccgccatt ccctctggcg
      961 tgttggccca acgcacatat tcctccgaca gctcaatgaa ccttatcggt cggacgacca
     1021 gcaagaacaa gagcttccaa gatgaatgcc gcacggaacc ctccatggca cataacgatt
     1081 gagatcagcc gcggcaggcg aggagcggag cgacgagact gacgtcggct ggagcgattt
     1141 gttgggaagc atacgattag taacccgtcc catttgcgac tgacgaatat acttttgtac
     1201 tgaacgcagt gacatgacga agaactggtg agtcttccgg attggactga ccggtctttg
     1261 aaaaacgctc acctacctga aaagcaatct gccattgtga actgcatagt aatgttccca
     1321 acactcctct cttctggagg atatgtgtgg ctaccccgct cataaatcga tcctgtgcgc
     1381 cggagtaata accgccggcg gcacacaatt cgtctaagtc atcagctgtt gcgttacctg
     1441 caagcatcgc agcaagtagt agaagaacaa gtcgcgggat cattcctgta acctctctag
     1501 tgatgcccaa cttcaattcg acaccatggt gtatgtcagc ctctccaggg tgtttaagcg
     1561 cagatgtggg tacatgatgc gccctccaat aaattggttg gctttcaggc agggatgtta
     1621 tgcgaaacga aataccccag ttcccaaacg gctctactgc ccccgtccgc ctccttgact
     1681 aggtacggga gcgcttgcgg ctcaagcatt attccatccg caccgagaaa tcgtatgttg
     1741 gctggattaa acgttatatc ctctttcacg acaagcggca tccgcgtgac atgggtgcaa
     1801 cgcatgtcga gcgatttctg acgcaccttg ccgtcgatcg ggagctgagc gcctccacgc
     1861 agaaccaggc acttgcggct cttctgtttc cctatcgcga ggtgctggga ctggagctgc
     1921 cctggctcga ggcgctggtg ctgcgcgtgc tctacggtac cggtatgcgc ctgatggaag
     1981 aggtccgtct gcggatcaag gacgtcgatt tcgttcgtaa cgagatcctg gttcgcgaag
     2041 gcaagggcgg gaaggatcgg gtgacgatgc tgctgcggtc gctctcggca ccgccgcaga
     2101 gatccgcaag ccggcatcgg gacatacctt gcgacatggc ttcgccacgc acctctgcgc
     2161 cgtgtcgtcg tcgggctgaa gctcgaccta caggggcggt cgcgggcatg gcccgctcct
     2221 acgcgaaaag ccggcgccgg tcagccgccg gtggtgttca tgaaccgcac gatctgcggc
     2281 tcgccgtcga gcgcgaaatg gtggcgctcg ggcttcaggt ccatcgcctg cacgatcgcc
     2341 tcgcgcagcg cagtgcggtc gccggggcgc gcgcgcatca cggcgcgcag gtccgcggag
     2401 tgctcgttgc cgaggcagag cagcagacgc ccttcgacgg tgaggcgcac gcggttgcac
     2461 aggtggcaga agttgtggct gtgcggcgag atgaagccca cgcgcgtcgc gctgtcggcc
     2521 atgcgccagt agcgcgaggg gccgccggtg ctgtccagcg agggcgtgag cgggtagcgc
     2581 gtctcgatga tcgcgcgcag ttcggcgctg gagcagaagc tcgtcgcgcg gtcgtgctcc
     2641 tctatgtgcc cgagcggcat ctcttcgatg aagctgatgt cgatatcgcg cgcgcgcgcg
     2701 aattccacca gcgccagcac ctcgtcgtcg ttgcgcccgc gcaggatcac gctgttcagc
     2761 ttcaggcgct cgaagccggc ggcgagcgcc gcgtcgatgc cggcgagcac agtgcccagg
     2821 tcgccggtgc gcgtgagctg gcggaaacgg gcgcgatcga ggctgtcgag gctgatgttg
     2881 atgcgcttca cgccggcttc gcgcagcggc gcggcgagcc ggggcaattg cgagccgttg
     2941 gtggtgagca ccagctcgcg caggcccggc agcgccgcga tgcgcccggc gagccacacg
     3001 atgttctgcc gcaccagcgg ttcgccgccg gtgaggcgga tcttggtgac gccgagcgcg
     3061 acgaaagcct cggcggcggc cagcagttcc tcgaggctga ggatctcgcg cctgggcagg
     3121 aactgcatat cctcgcccat gcagtagacg cagcggaaat cgcagcgatc ggtgaccgag
     3181 agccgcacgt aatcgacgcg gcggccgaag cggtcgatca gcgccggcgc ctgctgcacg
     3241 gcggcggcgt ccggcacaat cgcgttcatg acggcgcggc ccgcacctcg accggcacgc
     3301 cgttcacgat gctggtgccc gaggggaggt cgagaccgtc gccgatcagg tcgttggcgc
     3361 tgacgccggc atgcgcctgc gcgtgtggct ggccggtgtc ggcgtggcgg tgtccgaagc
     3421 cgtgcggcag gctcacgaca ccgggcatca tctcgtcgct gatctccacc ggcaccgcgc
     3481 gctcggccgc cgcggagcgc accagcgccg tggcgccgtc ggaaagcccg cgcgcggcgg
     3541 cgtccccggg atgcatgcgc agcgtgcaac ggttcctgcc gcgcacgaag tgcggcaggt
     3601 tgtgcagcca ggtgttcatg tcgcggatgt ggcgccgccc gatcagcagc agccccgacg
     3661 gcgctccggc gcgcagccgt tcgtgcaggc gcccgaggtc ctcctccagc agcgggtgca
     3721 gcagccggat gcgcttgtcc tcggtgcgca ggatgccggg caggcgcccc ggcgtcagcg
     3781 ggccgagatc gataccgtgc tcggcggcct tcacgcgcgc cagcgtcaac ccctcgccac
     3841 cctcgaaacc gtcgccgtag gggccggcgc gcagcatcat gtcgagcagg cgctcggggc
     3901 cggcctcggt gccggccagc tccttgacgc gctccgcccc caccgcggga agccgcgcgc
     3961 cgacacgctc ggccaggccc tcgaagatca tcgcgtcgag ctgctccacg gtcagcccgt
     4021 tcacgcggcc gcagatctcc agcatcagct gccactggtc gcgcgcaccg ggctcgggcg
     4081 tgagcacgcg cggcgagtag cgcgcgaagt tgcgcacgct ggtgctctgg aacaggaagt
     4141 cgtagttgct gtgctcgagc tgcaccgtcg agggcaggat cacgtcggcg cggctggtgg
     4201 tctcgttgat gtagatgtcg agcgcgacca cgaaatcgag cccgtccatc gcctcgcgca
     4261 gccggtcgcc gttcggcacg ctgagcaccg ggttgcccgc caccgtgatc agggcgcgcg
     4321 cgcggtccgg gccgtcccat gccagctcct cggccatcag cgtggccggc aacatcgaca
     4381 tgtactcggg gaagccgcgc accttcgagc gccagcgccc gcgcaatagc tggccgaccg
     4441 agtcgctgtc ctcgccttgg cccgtcgcgg gacgggggaa catggccccg ccctcggcgt
     4501 cgaggcgtcc gcgcagcagg ttcaccacgt cgaccagcca cgaggccagg gtgccgaact
     4561 cctgcgtgca caggccgatg cggccgtaga tcaccgggcg ttcggccgcg cagaagcgct
     4621 cgcacaggcg gcgcacgtcc gcgggcgcga cacccgtgat gccggcgacc gcttcgggcg
     4681 tgaacggcgc ggcgagctcg cgcaggcgct ccagcccgtc gatgcgtccg gcgagatggc
     4741 cgaggtccac gcgatcactc gcgaacagca ggttgaccag cgcgaacagc aggaacgcat
     4801 cggtgccggg ccggatgaag aggtgccagt cggcgattgc cgcggtctcg ctgcgccgcg
     4861 ggtcgatcac gacgagctcg ccgccgcgcg cgcgcagcgc ctcgaagcga ccaccggcgt
     4921 ccggcgccga catcaggctg ccctgcgaga ccagcgggtt ggcgcccatg cagacgaaga
     4981 agtcgctgcg gtcgagatcg gggatcggga aggtccacgc gctgccgtag agcagctcgc
     5041 agctcaggtt cttcggctgc tggtccaccg agccggcgga aaagaaacgc tgcgtctcga
     5101 gcagcgagat gaacagctgc gtgtagatct gggcgccgaa gttgtggccg ttcgggttgc
     5161 cgtagtacat cgcgacggcg ttgttgccgt gcgcggcgcg gatggccgcg aggcgctcgc
     5221 ccaccagcgc cagcgcggcc tcccactcca tttcctccca gccgttctcc gtgcgccgca
     5281 cggggcgacg caggcgcgcg ggatcctcgt ggagatcctt gacgacctgg gacttggcgc
     5341 agacgtagcc gcgcgagcga tggtcctcgg cgtcgccgcg caccgacacc acgcggttcg
     5401 cggcacggtc cactgccacg gccagcccgc agctggcctc gcacaccgga caggaacgga
     5461 aaacggtttc cgtcgcctgc ccgggagggc cctcaggtgc ggctgtggct tccatcgcag
     5521 agcggcttgt tggccgtcgc cttgcacgcg cagaagtacg cggtgcgcgt ctccggggcg
     5581 tcgtacttca gcggcgtgaa gccggtgtcc ttgtgcgagc cgtcgcagaa gggctgcttg
     5641 gcggaacgcc cgcagctgca ccaccagtag ctcttgcctg cctcgacctc gacggcgatg
     5701 ggggcatcgc cggcacgaac gggagcgctc atggatgttg ctccgggttc agagtacggc
     5761 gagtattttt tcggcggccg acacgtcgag gccagggccc ggctccaccg ccaggtgttt
     5821 gaccacgccg ttctcggcaa ccagcgcgaa acgttgcgag cgcgtgccca tgccgaagcc
     5881 gctgccgtcc agcgtgagcc ccagcgcctt ggcgaactcg gcgttgccgt cggccagcat
     5941 cagcagctcc tcggcgttct gcgccttgcc ccaggcgccc atcacgaagg catcgttcac
     6001 cgccatgcac gcgatggtgt ccacgccctt ggccttgatc ttgtccgcgt tcaccacgta
     6061 gcccggcagg tgcgccgcgc tgcaggtggg cgtgaacgcg cccggcaccg agaacagcac
     6121 gaccttcttg ccgttgaaga tatcgtcggt gctgatgctc tcggggcctt tctcgcccat
     6181 cgtcttcagg ttcacggaag ggatgcggtc gcctaccttg atcgtcatgt ccgttctctc
     6241 cggttggtgg cactgaaaca gtggccgtat ggtagcagag cgctttgcgg atattcaccc
     6301 gccgcggcgt gcgggtatat ggggtcagtt caccagcagc acctgacagc gcaggtgcga
     6361 caattcctcg agcacggcgc gccggtcctt cgccggtgtc aggtccccgg cgatcagcac
     6421 catgttgccg agccggttct cgacctcgcg cagcatttcc aggctgtcgg tattgaccgg
     6481 atgcgggtgc agattgacgc gcagcccggt cttcccgatc tcggcgagct gcgcggcgtc
     6541 gacctcgccg gcggcgatca cctccagcac gtggtggtcc tgctgcgcga ggcgtgcggc
     6601 gagttcgagc gcgcgcgtgc tggccggcgt ggcgccgcag agcacgaaca cccgcggcgg
     6661 gcgcgtctcc tggatgcgca cgcgcacgat ggtgcgctcc ctcggctgca tcagcagcag
     6721 ctcgacgcgt gccgacgcct cgctcagcgc ctcgccgcga cgcgcgcgcg tgacctggaa
     6781 ctcgcccttt acccggctgc gccgcgtgac cgcgctgaag cgctgctcgg cctcccgcga
     6841 caggcagcgc agcgcctgct cgacgtggga cccggtgatc gcgcgctcgc gccccgaacc
     6901 gagcgagatc tcgtgcgtga tcggcaggtc cgccacggcg tacacatccg cgtcctcgac
     6961 gaacagcgcg tcgagctcgg cctcgagctt ggcggcgatc agcgccgcgg cctccatcag
     7021 cgccgcctcg cagttcatcg cgtcgagcgc cagcatgatg cggcggatct tcgccttctg
     7081 cccgccgtcc tgcgcgctca tggcttgccg ttgcccgcgg ccttgtcatc gccgctgccg
     7141 gagtgcttgc gcccgatctc ggccagctca tgcagccgcg cgcgcacccg ctcgttgacc
     7201 gagtcggtct cgaagctgcc gtcctcccgc atcgcgccgg ccggcatgcc ggtcagcagc
     7261 gcgatcacgt catcgaccgc gctcgcggcg tggacgtgga aatttcccgc gcggcacgcc
     7321 tcgaccacct ccggcttcag catcaggtgc tgcacgttcg aggccgggat caccacgccc
     7381 tgggtgccgc tcagcccgcg cgcggcgcac acgtcgaaga aaccctcgat cttctcgttc
     7441 acgccgccga tcgcctgcat ctcgccgagc tggttcaccg agccggtcat cgcgagatcc
     7501 tggcggatcg ggacgccggc gatcgccgac agcagcgcga ccagctcggc catcgaggcg
     7561 ctgtcgccgt cgacgcgccc gtaagactgc tcgaacacga tgctcgccga cagcgcgagc
     7621 ggctggctct gcgcgtagcg cctggcaagg aagctggaaa ggatcatcac gcccttggaa
     7681 tgcagcggcc cgccgagctc ggtctcgcgc tcgatgtcga tcagcttgcc cgcgccgagg
     7741 cgcgcggtcg cggtgatgcg cgagggcctg ccgaaggcgt aatcgccgag ctggatcacc
     7801 gacaggccgt tgatctggcc gacctgcatg ccatcggtgg cgagcagcat cacgcccctg
     7861 tggatctgct cggccagcag ctcctggtag cggttgttgc gaaagcgccg ctgcgcgatg
     7921 gcctggtcga cgtggcgcgc ctcgatcagc gcgatgttct cgcgcgcggc ccagaaatcc
     7981 gcctgcagga tcaggtcgac caggtcgccc aggtgcagcg acagcttgtg gctgtcctcg
     8041 gcgtcgcgcg cgcactggtc gatcacgcgg gccacgccgc cggccgagag cgcccgtgcc
     8101 ttttcgcgcc gcgccagcgt cgcgatgaca cgcgcgtaca gccccacggc ctcgggactg
     8161 cggtccatgc tgtcgctgaa atccgcgttg accttgaaat acaggctgaa ctccgggtcg
     8221 tattccttca gcaggtgata gatgtagcgg tcccctacca gcacgatctt cacgtcgagc
     8281 gggatgggct cgggttcgag cgaggtggtc gacaccatgc tgaggatgcg ctcgatcgat
     8341 tcgatgcgga tctcgcgcga gcggatggcg cgcttgagcg cgtcccacac gaaggagttc
     8401 atcagcacct tgcgcgcatc cagcaccagg tagccgccgt tggcacggtg cagcgcgccg
     8461 ggcttgatca ggctgaagtt ggtgaccagc gtgcccatct gcgcgatgtg ctcgatgcgc
     8521 ccgatcacgt tctgcagcgt gggattgtct tcgtagagca ccggcacgtc ggcggcgccg
     8581 tcgtggtcca ccagcacgtt cacgtggtag cgcaggtgct ccatcgcgaa gatcttcttg
     8641 cgcagcggcg tgctgccttc ctgctgctcg ttgggcatga aatcccagat gttctcggcc
     8701 atgtcctggt gcgccgcgcc gaggaactcg agcaccgacg gatacatctg atagcgcgac
     8761 ttcagcgccg ccagcatcgg gtcgatgatg tcgtggatga actcgttgtc gatgcccttg
     8821 acgcgctcgg tgctctcctc ctgccagtcg tggatgctct cgatgatcga cttgagcctg
     8881 cggttcagct cctcgatgac gttcttggcg agctcctgct cttccttggg catgtcgttg
     8941 aactgcgcgg gcgtcatcag cttgccctcg cgcaccgggc ctatggtgta gccgcccggc
     9001 gtgcggatca ccaccatgcc cttgccgcgc gcctcgtcgt tgagctcgcc gaagagcttg
     9061 ccctcgcgct cctggtattc ctcctggatg tcgctgatgc gccggcggta ctcgtcgcca
     9121 cggaacacgt tgggcaccgt gaccagcatg cgctctatca gcttctccat gtcgcgcttg
     9181 agctgcacgc ccttgcccgg cggcagggcg agcgcgcgcg gacgcgaggg atcctcgaag
     9241 ttgttgacgt agcaccagtc gctcgcctcg ccgcgctcac ccgcgtgacg cgcgagcagc
     9301 tggtcgacca gcgtgcgctt gccgatgccg gtcgggccgg cgacgtagag gttgtagccc
     9361 tcgcgctgga ttcccacgcc gaactcgatc gccgcgatcg cgcggtcctg ccccatcgcg
     9421 gaatccacgt ccggcagctc cgcggtcgag cggaacccga gcgcctgcgc gtcggtggcg
     9481 cgcgacagcg catcggcctt cagggtctcg acagccatgc tctgtgctcc ttgctcagtc
     9541 ggcgcaaccg atacaggtat cggcgtaata cagggccgcg aggcgctcgg gcgcgatggg
     9601 gccgccgcag cggctgcaca cggcatattc gccgcgatcc atgcgcgcca gggcgtgcac
     9661 gaccttctgc agttcgagac gaccctcgga ttcgatggcg tagagcgcat cgaggttttc
     9721 ctgttcgacc acctgctccg cgaaatcggc cgacaggggc tcgtcgcgct tgcgcagatc
     9781 agcgtcgatg cgccgcaccc tttcctcgag ctcatcgcgc ttggcgatca ggcgcgcgcg
     9841 caacgtctgc tggtccggca tgagtcctcc cttgtggctt ggtgtgtcgc ggcgggcggc
     9901 cgcggtcctc ggattgccca cagcataatg ccttcagtcg cgcgcgggaa attgcccggc
     9961 cagccgcacc agcgccgccc cggcgaggcg ctgcagcacc cattccgttt tggcctcggc
    10021 tccgatagat tggtacagac gcaacgccgg ctcgttccag tcgagcaccg accattccac
    10081 gcgcccccag ccacgctcct gtgccacgcg ggccgcccat gcgagcaggc acctgcctat
    10141 gccgcgcccg cgcgcatgcg gcaaaacgaa caggtcctcg atgtggatgc cgggcttgcc
    10201 gacgaaggtc gagtaattgg ggaaccagag aatgaagccg acgggcgcgg catcgagctc
    10261 cgcgatcgcc gcctcggcga acgggcgggc gccgaacagc gcatcctcga tgtcacccac
    10321 cgtggcctgg acctcgtggg acagtttctc gtaggcggcg agcttgtgga tgaaatcgag
    10381 caccagcgcg gcatcggggc gttcggcctg gcgcaggctg atgccgggca gggtcgtgtg
    10441 gatccgggag gaagtcatcg ctgtggatat ccttgaaacg gcaggacgcc ccggccggcg
    10501 ccattggctg gcgcggcggg gcacggcagg cgccagtcta gcggcaggcc gcgcacggcg
    10561 taagcgcacg ctgcgggcgc tgctcgtgct gctctgcctc ggcggctgta ccgacctgcg
    10621 cgtgacgcac caggtgcagg atccggcggc ggacatcgga tcgcagcagg ccttcggttt
    10681 cctgcgcgca ccgctggcgg ccgacgcccg cgcctcggcc gaggcgcgca cgatggattc
    10741 ggccgtccgc gccgccatca ccggcgcgct gcaggcccgg ggttacacgc cggccctgcc
    10801 cgggtacaag cccgcgctcg ggatcgacta caccctgagc gacgttgtcg gtgccaatgc
    10861 gcgccggctg gactcgcctt ccgactacag ccgcagctgg cgcgccggcg gcgcgggcga
    10921 cggcaccggc tcgatggacc acaccatcgc cgatgcggcc ttctaccgcg agctcacgct
    10981 caccgtgctg ctgttcccgt cctcgaccgg cgcgctggcc tgggagggta gcgcccggca
    11041 gtccttcccc ggtgacctac cgcagggtgc acggctacag gccgcgctcg accgcatgac
    11101 gcgcaagctg ctggcccagc tgcccccggc gctctgaggc ccggcttcgc tcgaaggggt
    11161 gaatcggcat gagcatcgtt gtggtctgat ttgcctaacg ccggcacccg cggttgcgct
    11221 aaggtgatgc cggctgaacc ggtgcggtgt ccgcgccgta caatcacaac aacacaacgt
    11281 gccagcggcc cacgcggtcg ctgccccccg agagaggatt acccatgcgt tttgcctatc
    11341 acgcctcgat gcccgccccc gaccagtacc tgccgctggc gcgcgcggcc gaggagctcg
    11401 gcttcgacag tttcacgttc cccgacagca tctgctaccc gaaggagagc aacgccaagt
    11461 atccctataa cgacgacggc agccgcgact tcctcgacgg cgtgcccttc ctcgaggttt
    11521 tcatcgcgat ggccgcggcg gccgcggtca caacgaagat ccgcatcacc acctcggtgg
    11581 tgaaactgcc gatccgccag ccggcgatcg tcgccaagca gctctccagc ctcgcggtgc
    11641 tcagcaacaa ccgcatcgcc ctcggcgtgg gcctgagccc gtggccggaa gacttcgagg
    11701 cctgccagat cccctgggag aagcgcggtc agcgcatgga cgagatgatc gagatcattc
    11761 gtggcctgat gaagggcgat tacttcggct acaagggcca gatcttcgaa atggagccca
    11821 taaagctctg tccggtgccg agccagccgg tgccgatcct gctcggcgga cacgcggatg
    11881 ccgcgctcaa gcgcgcggca cgcatcggcg atggctggat cagcgccggc acctcgctcg
    11941 aggaactcga cggatacctg aagaagctgc acgccttccg cgccgagttc ggccgcgaca
    12001 aggagccttt cgaggtccag aacatggggg cctcggccta taccccggac ggcatcaagc
    12061 agctcgagga ccgcggcgtg ggcgaggtga tcgtggcctt ccgcgacgtc tacaagaagc
    12121 agcccgacac ctccctggag gagaagctcg ccacgatgcg ctggtacgcc gacaacgtga
    12181 tcgccaagac ccgctgaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 aaaaaagggg gcgaaagccc cctttttcgt gcaccgttgc cggcttactc cggcatgtgc
    12361 ggaccggccg cgatgatcga ttccgacacg ccgctgaact tgcggaagtt ctgctggaac
    12421 tggccgatca ggttgcgcat ctgcgcgtcg taggcggcct tgtcttccca ggtgttgcgc
    12481 gggttcagca ggtgctcctc gacgcccgcg acatgcgtcg ggacgtccag gttcagcccg
    12541 ggcaggtgct ccgtgggtgc gcccgccagc gccccgctct ggattgccga gatgatcgcg
    12601 cgcgtggtcg ggatcgggaa acggaagccc gcgccgccaa cgccgcccga gccgccggtc
    12661 cagccggtgt tgaccaggta gacctgactg ccgaattttt cgatgcgacg gatcagcagg
    12721 tcggcgtaga catgcgccgg gcgcgggaag aagggcgcgc cgaagcaggt cgagaacgtc
    12781 gacttgatgc cgctgcccga acccatctcg gtggagccga ccagcgccgt gtagccgctc
    12841 aggaagtggt acgcggccgc ttccttggac aggatcgaca ccggcggcag cacgccggtc
    12901 acgtcgcagg tcaggaagat cacggcgcgc ggctcgccgg cggcgatgcg ctcggcgcgc
    12961 ttctcgatgt gctcgcgcgg atagcagcag cggccgttct cggtgagggt cgtgtcggtg
    13021 taatccgcga tgcggtgatg ctcgtgcgtc accacgttct cgacgatcgc accgaagcgg
    13081 atcgcgtccc agatcagcgg ctcgttcttg cggctcaggt tgatggtctt ggcgtaacag
    13141 ccgccctcga tattgaacac cgcgccctcg gcccagccgt gctcgtcatc gccgatcagg
    13201 tagcgctcgg ggtcggccga cagcgtggtc ttgccggtgc ccgacagacc gaagaacagc
    13261 gcgacgtcgc cgtcgtggcc cacgttggcg gcgcagtgca ttggcagcac gtcgcgctcg
    13321 ggcagcagga agttctgcac cgagaacatc gccttcttca tttcgcctgc gtagcgcatg
    13381 ttggccagca gcaccttgcg ctgcgcgaag ttgatgatca ccgcgccgtc gctgttggtg
    13441 ccgtcacgct cgggatcgca gaggaacgac gggatattga gtacctgcca ctcctccttg
    13501 ccgcgcgggt tgtatttctc ggggcggatg aacatgttgc gcgcaaacag gttctgccac
    13561 gcgttctcgg tggtgacgcg gatcgggatg taatactcga ggtcggcgcc cacgtgcagg
    13621 tgtgacacgt aacggtcgtg ctcggagctg aactcgacca cacgatccca cagcgcgtta
    13681 aatttctcgg cgtcgaactt gcggttgatc gcgccccagt cgatggcgtc gttggagctc
    13741 ggctcgtcga cgatgaagcg gtcggccggc gagcgccccg tgcgccgccc ggtcgtcacg
    13801 accagtgcgc cggtgtcggc caggtgaccc tccttgcgac ggatcgcgtg ctccaccagg
    13861 agcgccgagc tcaggtccgt gtacaacgtg ctggtcgaaa tcgtcgcagt ctccattcag
    13921 aaaatcctat gttgctcaat ggattgggcg gctgttacgc cggcccgggc gggcgcggat
    13981 catacggtgg ccgcgcgcgg ggaggcgcat tatggcaaaa accccgggct tcaggaaacc
    14041 gttaaggaag tcaatgcttc gcggcgggaa cctcgacgtg gaacatcgcg tcgatcgcgg
    14101 cggcgtcgaa gcggtactcc tgctggcaga actcgcagtg gatcaggatg gttccctgct
    14161 cggcgagtat cgcccgcgcc tcgggctcgc cgagcgactg cagcgttgcg cccgcgcggc
    14221 tcgaggagca gcggcagccg aagcgtaccg gctccggcgc ctgcacccgc acgcgctcct
    14281 cgtggaacag gtgaaagagc agcgtctcga acgcctcgcc cagcaactcc gcgtcgctca
    14341 cggtgcacgc cagctggtgc aagtgttccc agcgcgccgc ctggtcgtcg accgccgtgg
    14401 cgctccccgg caacgcctgc aacagcaggc cggcggcatg ttcgccgtcg caggcgagca
    14461 ggatcgaggt cgggagctgc tcggactgcg tgaagtagtg ctccaggcag cccgcgaggc
    14521 gcggcgtgtc gagcggcacg atgccctggt agcgctggcc ttcggccggg gtgatcgtga
    14581 tcgccagcgt gccgcggctc agcagttcgt ccagcccgcc gcccacggca ccatcggcca
    14641 cgcgcgcata gccgcgcacc tggcggtcgt gggtgcattc gccgaagagg atggacaccg
    14701 gcccctcgct cttcgcctgc aggctgaggg atccggcgaa cttgatcgtg gagctcagca
    14761 gcacgctggc cgcgagcgac tccccgagca gcgcggccac gggccccggg tagaaatgct
    14821 ggcccaggat ctcgcgcagg ctggggccga ggcgtaccag ctcgccgcgc accgcggcag
    14881 tttcgaaatg gaaccgttgc agggtatcgg tatcgggcgg gctcatgagt cggagtggcg
    14941 ttccttgaac tggtggatga gccggcggtc gcgcttgctg ggacgtccct cgtgcaggat
    15001 atcgccggca cgctgcaggc ggcgctcgtc cgcaccggcc gtgcgcgccg cgaggctggc
    15061 ggcggtctcc tcgtagagca gggcggcctc gggcgccccg cggcgctctt cggacagcgc
    15121 gcgcacggtc acggtcttct cgtcgaagcc ctggcggatg cgcagctcgg caccgacctg
    15181 cacctccttc gagaccttgg tgcgggcgcc gtcgtaccag accttgccgc cctcgatggc
    15241 ctgcttggcg agattgcggg tgcggaagaa gcgcgccgcc cacagccact tgtcgaggcg
    15301 cacgcgctcc tcagacatcg acgtcgtcca gctccggcat gatctcgtcg aagtgcagta
    15361 tcgcggggaa atacaggccg gcacgcgcct cgcgcctgct gtcgggctgc agcagcgtca
    15421 gcaggtagcg gatgccgtac tcgtgggccg actgcagcac cgcgtggttg tcgtcgatca
    15481 gcagcgtgtg ctcgcgctcg aagggctcga ccgaagccag tgcggcccag aagcccggct
    15541 gctccttggc caggttgaag tcgtgcgagc agatgatgcc gtcgaaccag cgttccagcc
    15601 cggtctgctc cagcttcagg ctgaggctct tgtgatgcgc gttggtgacc agcagcacgc
    15661 gccggcggct cgcgtgcagg cgcgccagga aatcctcgac gtgcgggcgc acggcgatca
    15721 ggtggcgcat ctcttccttc atggcgcgca cgtcgagccc gagctcgcgc gaccagtaat
    15781 cgatgcaata ccagttcagg ttgccctgct cggcgcgaaa gcgttcgacc agctcgagcc
    15841 gcgcctcctc ctgcttgcgg tcgaagatct cggcgtagcg gcgcggcagg tgctcgagcc
    15901 agaagaagtt gtcgaaatgc aggtcgagca gcgtgccgtc catatcgagc agcacggttt
    15961 cgatgtcgtt ccagtcgacc atgatccacc tccgtgacag gggcgcagtt tacgggcaac
    16021 gcgcgcgggc gtcaccgcgc gcggcccggg acggcgccgc ggcgcatggc ctatagtgtc
    16081 atcgcatgag cgtcatactc cttcgggcac ccgcatgagc gaccatcccg ggccggaaat
    16141 actcgcctcc cgctgggtca gccacagccg cctgttccac gtcgaggagc tgcggctgcg
    16201 cttcggcaac ggcgtcgagc gcacctacga gcggcttaac cccggcttcc atggcgccgt
    16261 catggtggtg cccgtgcgtg acgacggccg gctgctgctg gtgcgcgaat acggtgccgg
    16321 tgtgggcgat tactatctct cctttcccaa gggggccgtg catgccgagg aatccatgct
    16381 cgacgccgcg aaccgcgagc tcatggagga ggtgggcttc ggtgcccatg aactggagtt
    16441 gctgaccgag ctctcgctgt cgccctccta catgggcaac cgcatgagca tcgtgctggc
    16501 ccgcggcctg ttcgagaagc gcctgccggg cgacgagccg gaaccactga cagtgagcgt
    16561 tcacacgttt gacgagctga tcgagcttca agccagcggc gtgctgaacg aggcctacgc
    16621 ggtcgcggca ctgttccttg cgcgcgagcg cctcgcggcc gcggaggctc cctgaaccgc
    16681 ctgccctcgg gctggcgcga ggcggtgttg gcggccgcac gcgaggccgg cgccgcgatc
    16741 ctgcccctgc gccgtgccgg ctccgacgcc gaccgcaagc ccgacggcag cctggtcagc
    16801 gccgccgacc gcgcggcatc ggcgatcatc ggcgcgcgac tgcacgcgct cgatcccgcg
    16861 ctccccgtga tctgcgagga agccgcgcag gacaccgcgg gggccgcgcg gttctggttg
    16921 gtggatccgc tcgacggcac ccgcgagttc ctgcgtggca gcgatgtgtt cagcgtcaac
    16981 atcgcgctga tcgaggacgg ttacccctgc ttcggcgcga tccacctgcc cgccagcgac
    17041 ctcgactact ggggcgcctg cggcgcgggc gcgtggcgtg acggctgtgc gatccgggcg
    17101 cggcgcctcg cggaggccgg cgcggggctg cacgccctgg tcagtccggg cgagcgcgac
    17161 agcgcgccgc gcgaattcgc ggcaacgctc gcgcgccatg gcggcacgca gcgcatcgag
    17221 g
//
LOCUS       DAORUQ010000055        17167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3025_length_17167_cov_6.204067, whole genome shotgun sequence.
ACCESSION   DAORUQ010000055 DAORUQ010000000
VERSION     DAORUQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17167
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3025_length_17167_cov_6.204067"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLW13_06790"
     CDS             complement(<1..401)
                     /locus_tag="PLW13_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016498216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQY70115.1"
                     /translation="MSSVKKVVLAYSGGLDTSVIVRWLQETYQCEVVTFTADIGQGEE
                     VEPAREKAKLLGVKEIHIEDLREEFARDYVFPMFRANAIYEGEYLLGTSIARPLIAKR
                     LVEIARDCGADAISHGATGKGNDQVRFELGA"
     gene            597..1646
                     /gene="pyrC"
                     /locus_tag="PLW13_06795"
     CDS             597..1646
                     /gene="pyrC"
                     /locus_tag="PLW13_06795"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQY70116.1"
                     /translation="MQELLILQPDDWHLHLRDGAALALTVNATARVFRRAIVMPNLKP
                     PVTATAEALAYRARILGALDPAVSRDFEPLMTLYLTDNTPPAEIARARDSGCVHAVKL
                     YPAGATTNSDAGVTDLGRCRPVLAEMERLGMPLLVHGEVTSPDTDVFDREKVFIDTVL
                     APLLGDFPALKVVFEHITTADAVAFVEAGPPNLAATITPHHLLYNRNHMLAGSIRPHF
                     FCLPVLKRNTHQRALVRAATGGSPRFFLGTDSAPHARATKECASGCAGIYSAHAALES
                     YAAVFEAEGALARLEGFASLHGADFYGLPRNTRRIRLRRESWQAPASLAFGDDTLVPM
                     CAGETISWRVGAAEA"
     gene            1712..2329
                     /gene="rnt"
                     /locus_tag="PLW13_06800"
     CDS             1712..2329
                     /gene="rnt"
                     /locus_tag="PLW13_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HQY70117.1"
                     /translation="MAARFRGYLPVVLDVETGGFNARTDALLEVAATPLEMNESGDLV
                     CGPTFSHAVVPFADANIEQASLDFTGIDPYDPQRGAIDEGEALRSLFSDIRHAVRAAG
                     CKRAILVGHNAFFDHGFLFAAAERQQIKRNPFHPFSTFDTVTLAGMAFGQTVLAKACA
                     MANIEFSTRKAHRASYDCEKTAELFCVIVNRWQQALGWPPADPSG"
     gene            complement(2365..2694)
                     /gene="grxD"
                     /locus_tag="PLW13_06805"
     CDS             complement(2365..2694)
                     /gene="grxD"
                     /locus_tag="PLW13_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQY70118.1"
                     /translation="MSEDIMEIIKQQIEGNPIILYMKGSPNQPQCGFSARATQVLMAC
                     GERFAYVDILANPEIRANLPAYGNWPTFPQLWINGELIGGSDIIAEMYEKGELLPLVK
                     EAAAKNA"
     gene            2935..4128
                     /locus_tag="PLW13_06810"
     CDS             2935..4128
                     /locus_tag="PLW13_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQY70119.1"
                     /translation="MDPTSIMPTYKRLEVCFERGEGVWLYDTEGRRYLDALAGIAVCG
                     LGHAHPAVTRAIQEQAARLLHTSNVYGITRQRELADRLVALSGMDNVFFGNSGAEANE
                     CAIKIARMYGHGRNIEKPAIIVMENSFHGRTLATLSATGSRKVQAGFEPLVSGFVRAR
                     FGELEELAIIARNNSSIVAVLVEPIQGESGIRMAPEGYLEGLRALCDQHGWLLMLDEV
                     QTGNGRTGAMFAFQHTAIMPDVLTTAKGLGNGVPIGACLARGRAAGVFHAGNHGSTFG
                     GNPLACAAAQAVLDTLVADELCANAVRMGERLVQGLRARLAGIPGVVEVRGRGLMVGI
                     ELREPCAHLVQAALEQGLLINVTSDNVIRLLPPLVIQAAEVDQIIDTLAALIAAQAGG
                     AQSAA"
     gene            4160..5065
                     /gene="argF"
                     /locus_tag="PLW13_06815"
     CDS             4160..5065
                     /gene="argF"
                     /locus_tag="PLW13_06815"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQY70120.1"
                     /translation="MTARHFLTLLDLDPRELETVIARAIAMKADPQGAGRGTIAPNRV
                     MGMIFEKSSTRTRVSFEAGMTQLGGHALFLSPRDTQLGRGEPVEDTARVLSSMVDIVM
                     IRTFAHAIVQRFAEYSRVPVINGLTDDFHPCQLLADMQTFVEHRGSIRGKQVAWIGDG
                     NNMCQSYINAARQFDFELRIACPEGYEPRADLVRANAGRVTVLRDPLAAARGAHLLAT
                     DVWASMGQEAEQEKRAAAFANYQLDRRLLDVAAPDAIYLHCLPAHRGEEISAELMDAP
                     DTVIWDEAENRLHAQKALIEFLLNS"
     gene            5272..6432
                     /locus_tag="PLW13_06820"
     CDS             5272..6432
                     /locus_tag="PLW13_06820"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY70121.1"
                     /translation="MATSAEYELGEYAFPRGWFAVAEGEQVTRAPLNVHYFGQDMVLY
                     RGESGRVVMLDAYCPHMGTHIGKCRNSATVLNGTFLEGDSIRCPFHAWRFGPDGRCDH
                     IPYFNGPIPEKARLKSWPVEERWGIVFCWHDPEDQAPDFPLPEIPEWDDPRFVRWHGL
                     DHVADLHHPVEVFDNESDGPHLEYLHGAGKLQAYENEVDGHLYHQRETLIEGFSSTEE
                     SKVLMGDSSGVGHESGADRSPALRTMTTVGAYHGTGLMLARFVEANGVQLLCITPVND
                     GECRVMSAAMLRSPTGNTDPATDEQIRRTYAGLLVEGLRRDGEVWLNKKPALQILQLP
                     TDGPFGHGRRWYSQFFNPREKAAAILKNSTGRHTIRGIQPWSESEHARMVNG"
     gene            6483..7166
                     /locus_tag="PLW13_06825"
     CDS             6483..7166
                     /locus_tag="PLW13_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQY70122.1"
                     /translation="MHTDTTLSFQETVRARKSVRSFLSRPVPEAIIHEVLEDARRAPS
                     NANTQPWNVHIVSGDKLRQLTRALHEAIGAGRFTPDFSFDTGAFYGRYKERSFEQGQS
                     YHEAIGVARDDEAARKAAAALNYSFFNAPHVALLFMPAFGDSVRVASDIGMYAQTFLL
                     SLTARGLGGVPQTSLGFFAQTMREFLEVPDELRFLFGISFGYPDATAPCNRIRTGRDP
                     VSHSVTFHG"
     gene            7215..8264
                     /locus_tag="PLW13_06830"
     CDS             7215..8264
                     /locus_tag="PLW13_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017530118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY70123.1"
                     /translation="MEMRALGRTEVQLSSVGLGVMNFGAQTAEDDAFRILDMAFDAGI
                     TLFDTAENYPAPVAAATQGRSEEILGRWIAARRLRDRVIVATKVAGPGNAAGDMSHIR
                     GNKRRLDRANIVQAVDDSLRRLDTDHIDLYQVHWPERAVTTLGRSRYSCIPDSPALVP
                     IEETLEALGEQVQAGKIRHIGVANETPWGVMRYLAAARERGLPRIVAIQNGYSLLDRY
                     FELGLAEIAMREQVGLLAYSPLAGGLLTGKYVGAPAPIPGSRSSLSPGFSARFTDKKM
                     RATAAYTALARQHGLEPAAMALAFVRQQPFTTSVLMAASSPAQLELNLRSLELDLSKE
                     LLKQINAIHDDLPNP"
     gene            8319..9125
                     /locus_tag="PLW13_06835"
     CDS             8319..9125
                     /locus_tag="PLW13_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY70124.1"
                     /translation="MSEAPHLLVEEDGALLIATLNRPEKLNAMTGQMMALFEQALHRF
                     RDKPALKVMLVRATGRYFCSGADLRSGGGAPAPRTASAIRENHRLGVFGVHRIYDEME
                     HIEKPIVVAHHAMCVGGGLELSLSCDFRLAAKSAAYAFPEGLFGVLPASNGVSRLTRI
                     CGPHWARWLIMANKPADADMALGMGLVHQVFPDQSFEQDVMDFCRHLARQNGEQMGAA
                     KIAIELANDVSRDQARHVERMANSALMLNPDYLARLEAYVGNIGSAAEKG"
     gene            9164..9637
                     /locus_tag="PLW13_06840"
     CDS             9164..9637
                     /locus_tag="PLW13_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70125.1"
                     /translation="MKEQARKWAGPAVPPGSLAELADHHAIGRLARIYALGMDMRDYD
                     LARSAFAPDAMAAGKQGMEPIDQNLQFTYGVASSFHATQHVISNQYIELNGDEALVWS
                     YGVAHHKVAPGEQRDEIIAGVHYRDRCRRFAQGWLIVERSVANQWVDIGSPQRRS"
     gene            complement(9664..10602)
                     /locus_tag="PLW13_06845"
     CDS             complement(9664..10602)
                     /locus_tag="PLW13_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY70126.1"
                     /translation="MNLKRLGYFLRIAELGSMNRASEVLHIAQPALTRQIHLLEKELG
                     VALFTRTARGMQLTNEGEQLRAEVIGPLRQLDFAFQNARLPAGKVEGSVSLGMPPSVR
                     YVLAQPLIGRVSASEPAIKVRIIEGQADHLVEWLVGGQIDIALLYGPSPDDRIIDRAM
                     VVEDLMLVGCIESALSPDKPVNFKRLSRYPLILPGSRHGIMSTLEKNAYITKANIDII
                     LRSDSLELTKQLVMSGDGFTILPYSAFSKEHEAGLLRHARIENPVLTRQIVTAATAQC
                     RVPRIISRLDVLIQQEISTLAASGKWPGKLLVNPEA"
     gene            complement(10749..12230)
                     /gene="glpK"
                     /locus_tag="PLW13_06850"
     CDS             complement(10749..12230)
                     /gene="glpK"
                     /locus_tag="PLW13_06850"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQY70127.1"
                     /translation="MDVLIALDQGTTSSRCLAFDARGNVVALAQREFAQHFPQDGWVE
                     HDPEDIWQSTLQSFDAVVCELLGAGHAVVGIGISNQRETTLLWDRRTGTAVYPAIVWQ
                     DRRTADTCARLQQEEGLGERVAARTGLLLDPYFSATKLAWILDNVAGVRARAEAGELA
                     FGTVDTFLLWRLTGGRRHCTDATNASRTMLFDIHAQCWDEELLARFGVPRAVLPEVLD
                     SAADFGTVQAGLPAAGVPIAGIAGDQQAALIGQGCLAPGQAKSTYGTGCFLVLNTGAT
                     PVRSHNRLLTTLAYRLDGRPAYALEGSIFVAGAAIKWLRDALGLIASAGESELVALRA
                     PDPGGVYLVPAFTGLGAPHWDPAARGAILGLTRDSGAEAIVTAALQSVAYQTRDLLDA
                     MARDGTTLDVLRVDGGMAANSWMLQFMADMLGIPVSRPRITEATALGAASLAGYRLGM
                     LPTLDHLTAQCGEERRFLPAMPEPERARLYAGWLDAVARVRTT"
     gene            12363..13295
                     /locus_tag="PLW13_06855"
     CDS             12363..13295
                     /locus_tag="PLW13_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY70128.1"
                     /translation="MAKIELRHLRTLVALRDTGSLVDAADRLCLTQSALSHQVKDVED
                     RLQCSLFMRKTRPVRFTSAGQRLLALADEILPQIRAAEIDLDRLAGGETGRLFLALEC
                     HSCFQWLMPTINTFRHHWPDVELDLSSSFSFAPLQALARGDLDLVISSDPLELPGVHY
                     EPLFRYEVRLALSPRHRLAARAVIEPADLADEVLICYPVERARLDVFRHFLEPADIEP
                     ASVRTAELTMMIIQLVASNRGVAALPNWAISEYENKDFLVSRALGAEGLWSTLYAAIR
                     EEQREHPYMRDFMAFARQTCFSDLPGIQPLASVR"
     gene            13429..14127
                     /locus_tag="PLW13_06860"
     CDS             13429..14127
                     /locus_tag="PLW13_06860"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HQY70129.1"
                     /translation="MSRNRCVAALCISLALLLQGCGTTPEKPESPTITPAPAPEPLPQ
                     PPAETVTPPEQPPAPPAGPQEPEKPAPEPEPVVCTPPPPPPPPAPAKRPPPQTALAVL
                     GGIEQVRVDPPGLQLTARLDTGAAGSSLHALDVRAFERDGKTWVKFQLVDTGTGKSVE
                     VSRPVVRSVAPKDAAGSKRYIVSLKVTVAGIAQFTEFALTDRSSSAYPVLIGRNFLRD
                     QAVVDVGRRFTRPE"
     gene            14162..15694
                     /locus_tag="PLW13_06865"
     CDS             14162..15694
                     /locus_tag="PLW13_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HQY70130.1"
                     /translation="MKEKLKVYIVAFLLALLGLSMALYKHFALGFPLLPYEKVAVWTV
                     ESKVEFEASGAPVVVSLALPDSQQGIEIVNENFTTSGYGFAEIDQPDGSRRAQWTRRE
                     ADGKQLLYYRLQVYPAALDQAAEVIAPSSPVPPEFDSAKAASASALLDEIHAVSANTE
                     TFATQLLRALTVEPLSQNAAFLLGENRDPAFIAETALQLLSLAGIPNRIVRGLVLEDD
                     RRRQKLVDLIEVWDEDEWKIFDPRSARAGKPADFMIWRRGDVSLLDVEGGRESQVTFS
                     ILRSYRPARDIALANAVDNRAALIDFSIYSLPIEEQNAFKTILLVPIGTLVVAIFRIL
                     IGLRTSGTFMPILIALAFIQTTLLWGLLLFLIVVGAGLFIRFHLARLNLLLIGRISSV
                     VTVVIGLMSGISILSYKLGLNQILTITFFPMIILSWTIERMSILWEEEGPKEVAIQGG
                     GSLLVATAAYLLMSNALVAHLTFNFPELLLVQLALIMLIGQYTGYRLTELKRFTPLVE
                     NR"
     gene            15696..16679
                     /locus_tag="PLW13_06870"
     CDS             15696..16679
                     /locus_tag="PLW13_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HQY70131.1"
                     /translation="MRFANPFANPRRLRQVGLLGMNERNWFISRYNQRKLYPLVDDKL
                     KTKLLAEQRGLAVPKLYGVVREQHEVETMGELLDKYDSFVIKPSRGSGGKGIKVIVGK
                     RDDVFIKSSGDEVTHLDVTRHVSNILSGLYSLGGKPDVAMIEALVEMDPQFNGLSYEG
                     IPDIRIIVFMGYPVMSMMRLATHASDGKANLHQGAVGVGLDLATGRSLFAVQYNRPLE
                     LHPDTGLPLNTLTIPHWHELMCLGASCHEITGLGYLGADIVMDRNRGPLIMELNARPG
                     LAIQIANNTGLSPRIRRIAREAAANPQRPVEERVQFSMSTFGTEPALAKAV"
     gene            16751..17167
                     /locus_tag="PLW13_06875"
     CDS             16751..17167
                     /locus_tag="PLW13_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70132.1"
                     /translation="MNRHLCSALTVACLQLPVATLLAQEAAPGQVAGGGGLNCIPLKR
                     IQNTRVLDDRTILFEMTGNETLVNRLPHRCPGLGFEKSFGYKTSISQLCSQDIIWVVT
                     HMGSGLDRGASCGLGRFEPYVAPETDASDKGKLEPM"
BASE COUNT         2961 a         5861 c         5546 g         2799 t
ORIGIN      
        1 taggcgccga gctcgaagcg cacctggtcg ttgcccttgc cggtggcgcc gtgggagatg
       61 gcatcggcgc cgcaatcgcg ggcgatctcc accagtcgct tggcgatcag cgggcgcgcg
      121 atcgaggtgc cgagcaggta ctcgccctcg tagatcgcgt tggcgcggaa catcgggaac
      181 acgtaatcgc gcgcgaattc ctcgcgcagg tcctcgatgt ggatctcctt cacgccgagc
      241 agcttggcct tctcgcgcgc cggctcgact tcctcgccct ggccgatgtc ggcggtgaag
      301 gtcaccacct cgcactggta ggtctcctgc agccagcgca cgatcaccga ggtatcgagg
      361 ccgccggaat aggccaggac cactttcttg actgaactca tgtcgaacct ccgctcgaag
      421 gggctgcgca aaaaacgggc cggccattct agccgcgccg ccgtcgcgat cccatcgccg
      481 ccagcggcaa aaataaaccg aacggcccgc gctgcggccc ggcggtggca accgcggtac
      541 ctctgccacg gtttttccgc tagcatcgcg gcctccgacc ggaccgctgc gccccgatgc
      601 aagaactcct gatactgcaa cccgacgact ggcacctgca cctgcgcgat ggagcggcgc
      661 tcgcactcac ggtcaacgcc accgcgcggg tgttccggcg cgccatcgtg atgccgaacc
      721 tgaagccccc ggtaaccgcg accgcggagg cacttgccta ccgcgcgcgc atcctggggg
      781 cgctcgaccc tgccgtcagc cgcgacttcg agccgctgat gacgctgtac ctcaccgaca
      841 acacgccccc cgccgagatc gcccgcgccc gcgacagcgg ctgcgtgcac gcggtcaagc
      901 tctaccccgc cggcgccacc accaacagcg acgccggcgt caccgacctc ggccgctgcc
      961 gcccggtgct ggccgagatg gagcgcctcg gcatgccgct gctcgtgcac ggcgaggtca
     1021 ccagcccgga taccgatgtg ttcgaccgcg agaaggtgtt catcgatacc gtgctcgcgc
     1081 cgctgctggg cgatttcccg gcgctgaagg tcgtgttcga gcacatcacc acggccgatg
     1141 ccgtggcctt cgtggaggcg ggtccgccga acctcgcggc cacgatcacg ccgcaccacc
     1201 tgctctacaa ccgcaaccac atgctggccg gctcgatccg cccgcacttc ttctgcctgc
     1261 cggtgctcaa gcgcaacacg catcagcggg ccctcgtgcg cgccgccacc ggcggctcgc
     1321 cgcgcttctt cctcggcacc gattccgcgc cccatgcgcg cgccaccaag gagtgcgcca
     1381 gcggctgcgc cggcatctac agcgcgcatg ccgcactgga gagctacgcg gcggtgttcg
     1441 aggccgaggg cgcgctcgct cgactggaag gcttcgcgag cctccacggc gcggatttct
     1501 acggcctgcc acgcaacacg cggcgcatcc ggctgcgccg cgagtcgtgg caggcgccgg
     1561 cgagcctggc gttcggcgac gacacgctgg tcccgatgtg cgcgggcgaa accatcagct
     1621 ggcgcgtcgg cgctgcggag gcctgacgat gaagcgcgag cgcaacgact ggctgctcga
     1681 agcggaagcg gggggcaatg gcgaaacgct gatggcggcg cgattccgcg gctacctgcc
     1741 ggtggtgctg gacgtggaga cgggcggctt caatgcgcgc accgatgcgc tgctggaggt
     1801 ggcggcaacg ccgctggaga tgaacgagtc gggcgacctg gtgtgcgggc ccacgttcag
     1861 ccacgccgtc gtgcccttcg ccgatgcgaa catcgaacag gcctcgctcg acttcaccgg
     1921 catcgatccc tacgacccgc aacgcggcgc catcgacgag ggcgaggcgc tcaggagcct
     1981 gttctcggac atccgccatg cagtgcgcgc cgccggctgc aagcgcgcca tactcgtggg
     2041 ccacaacgcc ttcttcgacc acggcttcct gttcgccgcc gccgagcgcc agcagatcaa
     2101 gcgcaacccc ttccacccgt tcagcacctt cgacaccgtg acgctggcgg gcatggcctt
     2161 cggccagacc gtgctggcca aggcgtgcgc gatggccaac atcgaattca gtacgcgcaa
     2221 ggcacaccgc gccagctacg actgcgagaa gaccgcggag ttgttctgcg tgatcgtgaa
     2281 ccgctggcag caggcactgg gctggccgcc ggcggatccc agcggctagc cggatagtcg
     2341 tccggatgaa tccggagctg cggatcaggc gtttttggcc gccgcttcct tgaccagcgg
     2401 cagcagctca cccttctcgt acatctcggc gatgatgtcg gagccgccga tcagttcgcc
     2461 gttgatccac agctgcggaa aagtcggcca gttgccgtag gccggcaggt tggcgcggat
     2521 ttccgggttg gcgaggatgt cgacgtaggc gaatcgctcg ccgcaagcca tcagcacctg
     2581 tgtcgcgcgc gccgagaagc cgcactgcgg ctggttgggc gagcccttca tgtacaggat
     2641 gatcgggttg ccctcgatct gctgcttgat gatttccatg atgtcctcgg acatgtgctg
     2701 catccacctc gtgtgcaagt tgaaccggcc cggaagtcta cacgactccc ctgctataac
     2761 ccagtaaagc aatcgggaat taccccgcga cccgtgatgc agcgccggat ctgcggattt
     2821 gtacgcatac cgaaccttgc gtacgcggca gcgcaccggt agcatcggcc gcttttcacg
     2881 ggggcagcaa gggctgcctc tttgcgtttt ggccacccgg aaacgaggcg caccatggat
     2941 ccgacgtcga tcatgcccac ctacaagcgg ctcgaggtct gcttcgagcg cggcgagggc
     3001 gtgtggctct acgacaccga aggccggcgt tatctcgacg cgctggcggg catcgcggtc
     3061 tgcgggctcg gccatgcgca cccggcggtc acgcgcgcca tccaggagca ggccgcgcgc
     3121 ctgctgcaca cctccaacgt ctacggcatc acgcgccagc gcgaactcgc cgaccgcctc
     3181 gtcgcgctga gcggcatgga caacgtgttc ttcggcaact ccggcgccga ggccaacgag
     3241 tgcgcgatca agatcgcgcg catgtacggc cacggcagga acatcgagaa gcccgccatc
     3301 atcgtgatgg agaattcctt ccacggccgc acgctggcca cgctctcggc caccggcagc
     3361 cgcaaggtgc aggcgggctt cgagccgctg gtgagcggct tcgtgcgcgc acgtttcggt
     3421 gaactcgaag agctcgcgat catcgcgcgc aacaacagca gcatcgtcgc ggtgctggtg
     3481 gaaccgatcc agggcgagtc cggcatccgc atggcgcccg agggctacct tgaggggctg
     3541 cgcgcgctgt gcgaccagca cggctggctg ttgatgctcg acgaggtgca gacgggcaac
     3601 gggcgcacgg gcgcgatgtt cgcgttccag cacaccgcga tcatgcccga cgtgctgacc
     3661 accgcgaagg gcctcggcaa tggtgtcccg atcggcgcct gcctggcacg cggcagggcc
     3721 gccggcgtgt tccatgccgg caaccacggc tccaccttcg gcggcaaccc gctcgcctgt
     3781 gccgcggcgc aggccgtgct cgacaccctc gtcgccgacg agctctgcgc caacgccgtg
     3841 cgcatgggtg agcgtctggt gcaggggctg cgcgcgcgcc tggcgggaat cccgggcgtg
     3901 gtcgaggtgc gcggccgcgg tctgatggtc ggcatcgagc tgcgcgaacc ctgcgcccac
     3961 ctggtgcagg cggcgctgga gcagggcctg ctgatcaacg tcacctccga caacgtgatc
     4021 cgcctgctgc cgccgctggt catccaggcg gccgaggtcg accagatcat cgacacgctg
     4081 gccgcgctga tcgcggccca ggccggtggc gcacagagcg ccgcctgacc cctcgcaccc
     4141 ttcaccggaa gtacgcgcca tgacggcaag acatttcctg accctgctgg acctggaccc
     4201 gcgggaactc gagaccgtga tcgcgcgcgc catcgcgatg aaagccgacc cgcagggcgc
     4261 cggccgcggc acgatcgcgc cgaaccgcgt gatgggcatg atcttcgaga aatcctcgac
     4321 ccgcacccgc gtctccttcg aggccggcat gacgcaactc ggaggccacg cgctgttcct
     4381 gtcgccgcgc gacacgcagc tcgggcgcgg cgaaccggtg gaggacaccg cgcgcgtgct
     4441 gtcctcgatg gtcgatatcg tgatgatccg caccttcgcg catgcgatcg tgcagcgctt
     4501 cgccgaatat tcgcgcgttc cggtgatcaa cggcctgacc gacgacttcc acccctgcca
     4561 gctgctcgcg gacatgcaga ccttcgtcga gcaccgcggc agcatccgtg gcaagcaggt
     4621 ggcctggatc ggcgacggca acaacatgtg ccagtcctac atcaacgcgg cacggcagtt
     4681 cgacttcgag ctgcgcatcg cctgccccga gggctacgag ccgcgcgcgg acctggtgcg
     4741 cgccaacgcc ggccgcgtga ccgtgttgcg cgatccgctg gcggccgctc gcggcgcgca
     4801 cctgctcgcc accgacgtgt gggcctcgat ggggcaggaa gccgagcagg agaagcgcgc
     4861 cgcggccttc gccaactacc agctcgaccg ccgcctgctc gatgtcgccg cgccggacgc
     4921 catctacctg cattgcctgc ccgcgcaccg cggcgaggaa atcagcgccg agctgatgga
     4981 cgcgcccgac accgtgatct gggacgaggc cgagaaccgc ctgcacgcgc agaaggcgct
     5041 gatcgagttc ctgctgaatt cctgatcagg cgaacggcca gccaatccgg acagtaccga
     5101 tcccgtatca gccatatcat ttcgatatgg ctgagcgcca tttattgtct tggatctata
     5161 cccccgcgat agatattctg ttgctcgtcg tgatgcagcg caaagagcag gtcccgcgct
     5221 cgagcatgcc gctccctgac ccggcaaaac atgaatgtga ggggtgtccc ggtggcaacg
     5281 tcagcagagt acgagcttgg tgagtatgcc ttcccgcggg gctggtttgc ggtggccgag
     5341 ggcgaacagg tcacgcgcgc accgctgaac gtccactact tcggccagga catggtgctc
     5401 taccgcggcg agagcggccg ggtggtgatg ctcgacgcct actgccccca catgggaacc
     5461 cacatcggca agtgccgcaa ttcagccacg gtactcaatg gcaccttcct ggaaggcgat
     5521 tccattcgct gcccgttcca cgcatggcgt tttggtccgg acggtcgctg tgatcacata
     5581 ccgtacttca acggtcccat tccggaaaag gcgcggctaa agagctggcc ggtggaggag
     5641 cgctggggca tcgtgttctg ctggcatgat cccgaggacc aggcgccgga ttttccgctg
     5701 ccggagattc ccgagtggga cgaccctcga ttcgtgcgct ggcacggcct ggatcatgtg
     5761 gccgacctgc accatcccgt cgaggtgttc gacaacgagt cggacggccc ccacctggag
     5821 tacctgcacg gtgccggaaa gctgcaggcc tacgaaaacg aggtggatgg ccacctgtac
     5881 catcagcgcg aaaccctgat cgaaggcttt tcatcgaccg aggaaagcaa ggtgctcatg
     5941 ggtgactcgt ccggtgtcgg acatgaatcc ggggcggacc gttcacctgc actcaggacc
     6001 atgaccacgg tgggcgccta ccacggaacc ggcctgatgc tggcgaggtt cgtcgaagcc
     6061 aacggcgtac agctgctgtg catcaccccg gttaacgatg gcgagtgccg ggtgatgtcc
     6121 gccgcgatgc tcagatcgcc cacgggaaac accgatccgg ccaccgacga gcagattcgc
     6181 cgcacctacg ccggcctcct cgtcgagggc ttgcgtcgcg acggcgaggt ctggctgaac
     6241 aagaagccgg ccctgcaaat cctgcagctg ccgacggatg gacccttcgg gcacgggcga
     6301 cgatggtata gccagttctt caacccccgg gagaaggcgg cggcaatcct caagaacagc
     6361 acgggcagac acaccatccg gggcatccag ccatggagtg aaagcgagca tgcgcgcatg
     6421 gtcaacggct gacgggggca cgtgacgtgg actacagacc tgctttgcga ggcggcgacg
     6481 cgatgcacac cgacaccacg ctctcgttcc aggagaccgt tcgcgcaaga aaatcggtca
     6541 ggagtttcct ctcccggccg gttccggagg cgatcatcca cgaggtgctg gaggatgccc
     6601 ggcgcgcacc atccaacgcc aacacgcaac cgtggaacgt gcatatcgtt tcgggcgaca
     6661 agttgcgcca gctcacccgg gcgcttcacg aagccatcgg tgcaggccgc ttcaccccgg
     6721 acttctcctt cgacacgggg gccttctacg gccgctacaa ggagcgcagt ttcgagcagg
     6781 gccagtccta tcacgaggcg atcggggtgg ctcgtgatga cgaggcggcg cgcaaggcag
     6841 cggctgccct gaactattcc tttttcaatg cgccgcacgt tgcgctgctc ttcatgcccg
     6901 cgtttggcga cagcgtgcgg gtagccagcg acatcggcat gtatgcccag acgttcctgc
     6961 tgtcgctcac cgcgcgcggc ctggggggcg tgccgcagac atcgctcggt ttctttgcgc
     7021 agaccatgcg ggagtttctc gaggttccgg acgagctgag gtttctgttc ggcatatcct
     7081 ttggctatcc ggatgcgacc gcgccctgca accggatacg gacggggcga gaccccgtgt
     7141 ctcacagcgt cacgtttcat ggatagccgc tgcagcgaac accgtagggg cgtcggcagg
     7201 accaggagga tggcgtggaa atgagagctc tcggccggac tgaggtgcag ctcagttccg
     7261 ttggcctggg tgtgatgaac ttcggggcgc aaacggccga ggacgatgcg tttcgcatcc
     7321 tggacatggc gtttgacgcc ggcataaccc tgttcgacac ggcggagaat tatcctgcac
     7381 cggtagcggc ggctacgcag gggcgttccg aggaaatcct ggggcgctgg atagcggcgc
     7441 gcaggctgcg cgaccgcgtc atcgtggcaa ccaaggttgc cggacccggc aacgcggccg
     7501 gcgacatgag ccacatccgc ggcaacaagc gccgcctcga tcgtgcaaac atcgtccagg
     7561 ccgtcgacga cagcctgcgc cggctggaca ccgaccatat cgacctgtat caggttcact
     7621 ggccggagcg tgccgtcacc acgctcggac gttcacgcta ttcctgcatt cccgactcac
     7681 ccgcgctggt acccatcgag gaaaccctgg aggcgctggg cgaacaggtg caggccggaa
     7741 agatcaggca catcggcgtc gcgaacgaga cgccgtgggg cgtcatgcgc tacctggccg
     7801 ccgcccgcga acgcggcctg ccgcgtatcg tcgccatcca gaacggctac agcctgctcg
     7861 atcgctattt cgagctgggc ctggcggaaa tcgcgatgcg cgaacaggtc ggtctgctgg
     7921 cctattcacc cctggcaggt ggcctgctga ccggaaaata cgtgggcgcg ccagcaccga
     7981 taccgggcag ccgctcgagc ctgtcccccg gctttagcgc gcgcttcacg gacaagaaga
     8041 tgcgagcgac agccgcttac accgcgctgg cccggcagca cggactggag ccggccgcca
     8101 tggcgctggc cttcgtccgg caacaaccgt tcacgacatc ggtgctgatg gccgccagca
     8161 gtccagccca actcgagctc aatctgcgca gcctcgagct cgatctgtcg aaggagctgc
     8221 tcaagcagat caacgcgatc catgacgatc tccccaatcc gtgacggttt gtcgccctct
     8281 gcccgccacg tacccgcgcc tgagcgaagg agttgtcgat gtccgaagcc ccccatctgc
     8341 tggtcgagga agacggggcg ctcctgatcg ccacgctcaa ccggccggaa aaactcaatg
     8401 ccatgacggg gcagatgatg gcgttgttcg agcaggccct ccatcgcttc cgggacaagc
     8461 cagcgctcaa ggtgatgctg gttcgtgcga caggccgcta tttctgctcg ggcgccgatc
     8521 tgcgcagcgg cggcggtgcc cccgccccca ggacggcctc ggccatccgc gaaaatcacc
     8581 gcctcggggt gttcggggtc caccgcatct atgacgaaat ggagcatatc gaaaagccca
     8641 tcgtggttgc ccaccacgcg atgtgcgtgg gcggcgggct ggagctctcg ctctcctgcg
     8701 actttcgtct ggcggcgaaa agtgcggcct atgcctttcc cgagggcttg ttcggcgttc
     8761 tgcccgcttc caacggggtc agccgcctga cgcgcatctg cgggccgcat tgggcacgct
     8821 ggctcatcat ggccaacaag ccggcagacg ccgacatggc cctcggcatg ggcctggtgc
     8881 accaggtgtt tccggaccag agcttcgagc aggacgtgat ggatttttgc cgccacctcg
     8941 ccaggcagaa cggggagcag atgggcgcgg caaagatcgc gatcgaactg gccaacgatg
     9001 tgagccgcga ccaggcccgc cacgtcgaac gcatggccaa cagcgcactg atgttgaacc
     9061 cggactatct cgcgcggctc gaggcctatg tcgggaacat cggcagcgct gcagaaaagg
     9121 gttgagcagc agttatccga tcacgtgtcg acaggagata ttcgtgaagg agcaagcaag
     9181 gaaatgggca ggacccgctg tcccgccagg gagcctggct gaactggcgg atcatcatgc
     9241 aataggccgg ctcgccagga tctacgcgct cggcatggat atgcgcgact acgacctggc
     9301 ccgaagcgcc ttcgccccgg acgccatggc cgcaggcaag cagggaatgg agccgatcga
     9361 ccagaacctg cagtttacct atggcgtggc gtcgagcttt cacgcgaccc agcacgtcat
     9421 ttccaaccag tacatcgagc tgaacggcga tgaagcgctc gtctggagct acggcgtcgc
     9481 gcatcacaag gttgcacccg gcgaacagcg cgatgaaatc atcgcggggg tccactaccg
     9541 cgaccgatgc agacggtttg cgcaaggatg gctgatcgtc gagcgcagcg tggccaacca
     9601 gtgggtggat atcggctcgc cgcaacgccg ctcatagcca gcccgaacag cgagcgagag
     9661 ccgttaagcc tccgggttga cgagcagttt tcccggccat tttccgctcg cggcgagggt
     9721 cgatatctcc tgctggatca ggacatccag cctggagatg atgcggggca cgcgacattg
     9781 tgcggtggcg gcagtgacga tctggcgcgt gagcacagga ttctcgattc tcgcatgacg
     9841 gaggagaccg gcctcatgct ccttgctgaa agccgaataa ggcaggatcg tgaatccgtc
     9901 tccggacatg acgagctgct ttgtcagctc gagggagtcg ctgcgcagaa tgatatcgat
     9961 gttggctttc gtgatatagg cattcttttc cagtgtcgac atgatgccgt ggcgcgagcc
    10021 gggaagaatg agcgggtacc gggacagtcg tttgaaattg accggcttgt ccggggagag
    10081 cgccgactct atgcagccga ccagcatcag gtcctctacc accatcgcgc gatcgatgat
    10141 gcggtcgtca ggggacgggc catagagcag cgcgatatcg atctgtccgc cgaccagcca
    10201 ttcgacgaga tgatcggctt gcccctcgat gatacggacc ttgatggcgg gctctgacgc
    10261 agaaaccctg cctatgagtg gctgcgccag cacatacctg accgacgggg gcatccccag
    10321 ggacacgctg ccttccacct tgccggccgg aagtcgtgca ttctggaagg caaaatcgag
    10381 ctgacgcagc ggtccgatga cttcggcccg cagttgctcg ccctcgttcg tgagttgcat
    10441 cccgcgcgcg gttcgcgtga acaatgcgac gcccaactcc ttttccagca agtggatctg
    10501 gcgggtcagg gcaggttgcg cgatgtgcag cacttccgag gctctgttca tcgagccgag
    10561 ttcggcgatc cgcagaaaat agccgagacg cttgaggttc atggctcccc ctttccaacg
    10621 gcccattttg ccgtgaacgg gcaaagctgc cacgtgcaaa cgcagctccg tcaacggtag
    10681 tcgtgacgag gcgcggatca aggccagccg gttgcagagt tcggctgaag ccgaacctac
    10741 gcgggaacct acgtcgtgcg cacgcgcgcc accgcatcca gccagccggc gtagaggcgc
    10801 gcgcgctcgg gctcgggcat cgcgggcagg aagcgtcgct cctcgccgca ttgcgccgtg
    10861 aggtgatcca gggtcggcag catgccgagc cggtagcccg ccaggctcgc cgcgcccagc
    10921 gccgtggcct cggtgatgcg cgggcggctg accgggatgc ccagcatgtc ggccatgaac
    10981 tgcagcatcc agctgttggc ggccatgccg ccgtccacgc gcagcacatc gagcgtcgtg
    11041 ccgtcgcgcg ccatcgcatc gagcaggtcg cgggtctggt aggccaccga ctgcagcgcg
    11101 gcggtgacga tcgcctcggc gccgctgtcg cgcgtgagcc cgaggatcgc gccgcgggcc
    11161 gcgggatccc agtgcggtgc gccgaggccg gtgaatgccg gcaccaggta cacgccaccg
    11221 ggatcgggcg cccgcagcgc caccagctcg ctttcacccg cgctcgcgat cagccccagc
    11281 gcgtcgcgca gccacttgat cgccgcgccg gccacgaaaa tgctgccttc cagcgcgtac
    11341 gcgggcctgc cgtcgagccg gtaggccagc gtggtcagca ggcggttgtg cgagcgtacc
    11401 ggcgtggcgc ccgtgttcag caccaggaaa caaccggtgc cgtaggtgct cttggcctgc
    11461 ccgggcgcga ggcaaccctg cccgatcagc gccgcctgct ggtcgcccgc gatgccggcg
    11521 atcggtacac ccgccgcggg cagtcccgcc tgcacggtcc cgaaatccgc ggccgaatcg
    11581 agcacctcgg gcagcaccgc gcgcggcacc ccgaagcgcg ccagcagttc ctcgtcccag
    11641 cattgcgcgt ggatgtcgaa cagcatcgtg cgcgaggcat tggtggcgtc ggtgcaatgg
    11701 cgccggccgc cggtgagccg ccacagcagg aaggtgtcga cggtgccgaa ggcgagctcg
    11761 ccggcctcgg cgcgcgcgcg gactcccgcg acgttgtcga ggatccacgc cagcttggtg
    11821 gccgagaaat agggatccag caacagcccg gttcgcgccg cgacgcgctc gccaagaccc
    11881 tcttcctgct gcagccgcgc gcaggtatcc gcggtgcgcc ggtcctgcca cacgatggcc
    11941 gggtacaccg ccgtaccggt gcgccggtcc cacagcagcg tggtctcacg ctggttgctg
    12001 ataccgatgc cgacgacggc gtggcccgcg ccgagcagtt cgcacaccac ggcgtcgaag
    12061 ctctgcagcg tgctctgcca gatgtcctcc gggtcgtgct cgacccagcc gtcctgcggg
    12121 aagtgctgcg cgaactcgcg ctgcgcgagc gcgaccacgt tgccgcgggc atcgaaggcc
    12181 aggcagcgcg agctcgtggt gccctggtcc agcgcgatca gcacgtccat ggggtacccg
    12241 tcctcccggt ccgggcttga tccccggcgc atgcatcaat agtatttttc attcgatgct
    12301 gttccgacat gcatttcatt catcgcgtcg cctcggcggc gaacccgacg gacccattcg
    12361 ccatggccaa gatcgaactg cgccatcttc gcacgctggt cgccctgcgc gacaccggca
    12421 gcctggtcga tgccgccgac cgcctgtgcc tgacccagtc ggcgctctcg caccaggtca
    12481 aggatgtcga ggaccggttg cagtgctcgc tgttcatgcg caagacccgc ccggtgcgct
    12541 tcaccagcgc cgggcagcga ctgctcgcgc tggccgacga gatcctgcca cagatccgtg
    12601 ccgccgagat cgacctcgac cggctcgcgg gcggcgagac cgggcggctg ttcctcgcgc
    12661 tcgagtgcca cagctgcttc cagtggctga tgcccacgat caacacgttc cgccatcact
    12721 ggcccgacgt cgagctggac ctctccagca gcttcagctt cgcgccgctg caggcgctcg
    12781 cgcgcggcga cctcgatctc gtgatcagct ccgatccgct ggaactgccc ggcgtgcatt
    12841 acgagccgct gttccgctac gaggtccgcc tcgcgctgtc cccgcgccac cgcctggccg
    12901 cacgggcggt gatcgagccc gccgacctag ccgacgaggt gctcatctgt tatccggtcg
    12961 agcgtgcgcg gctcgacgtg ttccgccact tcctggagcc cgccgacatc gagccggcaa
    13021 gcgtgcgcac ggcggagctg acgatgatga tcatccagct ggtggccagc aaccgcggcg
    13081 tcgccgcgct gcccaactgg gccatcagcg agtacgagaa caaggacttc ctggtctcgc
    13141 gagcgctcgg cgccgaggga ctgtggagca cgctgtacgc ggccatccgc gaggaacagc
    13201 gcgagcatcc gtatatgcgc gacttcatgg cgttcgcgcg ccagacctgt ttcagcgacc
    13261 tgcccggcat ccagccgctg gcgtccgtgc gctgaggcgt ggctcccggt aaaagttgtc
    13321 acaaagccgc gccatcatgg tcgcgacaaa gcccgttgat tgccgcccgc ccgcgcgggc
    13381 tctatagttt gcgcagcctt gaacccatgc ggaatttcac cccgaatgat gagccggaac
    13441 cgttgcgtcg ccgcgctgtg catatccctt gcgctcctgc tgcagggctg cggcaccacg
    13501 cccgagaagc ccgaatcgcc cacgatcaca cccgcgccgg cccctgaacc cctgccgcaa
    13561 ccgcctgcgg aaacggtcac gccgccggaa cagcctccgg cgccgcccgc cggaccgcag
    13621 gagcccgaga agcccgcacc cgaacccgag ccggtggtat gcacgccgcc accaccgccg
    13681 ccgcctcccg cacccgcgaa acgccctccg ccgcagacgg cgctggccgt gctgggcggg
    13741 atcgagcagg tacgtgtcga tcccccgggg ctgcagctca ccgcgcgact cgacaccggc
    13801 gctgccgggt cctcgctgca cgcgctcgac gtgcgcgcgt tcgaacgcga cggcaagacc
    13861 tgggtgaagt tccagctcgt cgataccggt accggcaaaa gtgtcgaagt ctcgcgcccg
    13921 gtcgtgcgct ccgtcgcgcc gaaggatgcc gccggctcca agcgctacat cgtcagcctc
    13981 aaggtcacgg tcgccggcat cgcgcagttc accgaattcg cgctcaccga ccgctcctcg
    14041 tccgcctatc ccgtgctgat cgggagaaac ttcctgcgcg accaggccgt ggtcgacgtg
    14101 ggacgccgct tcaccaggcc ggaatagccg caccaactga atccgccggg gcggagctcg
    14161 catgaaagaa aaactgaagg tctacatcgt cgcgttcctg ctcgccctgc tggggctcag
    14221 catggcgctg tacaagcact tcgcgctcgg attcccgctg ctgccctacg agaaggtggc
    14281 ggtgtggacg gtggagtcca aggtcgagtt cgaggcgagc ggcgcgccgg tggtggtgtc
    14341 gctggcgctg cccgattcgc aacagggcat cgagatcgtc aacgagaact tcacgacctc
    14401 gggctacggc ttcgcggaga tcgaccagcc cgacggcagc cgccgcgcgc aatggacgcg
    14461 ccgcgaggcc gatggcaagc agcttctcta ttaccgcctg caggtctatc cggcggcgct
    14521 ggaccaggcg gccgaggtga tcgcaccatc gtcgccggtg ccgcccgagt tcgacagcgc
    14581 caaggccgcc tcggcctccg cgctgctcga cgagatccac gcggtgtcgg ccaacaccga
    14641 aaccttcgcg acccagctgt tgcgcgcgct gaccgtggaa ccgctgtcgc agaacgcggc
    14701 cttcctgctc ggcgaaaacc gcgaccccgc cttcatcgcc gagaccgcgc tgcaactgct
    14761 gtcgctggcc ggcatcccga accgcatcgt gcgcggactg gtgctcgagg acgaccgtcg
    14821 gcgccagaag ctggtggacc tgatcgaagt ctgggacgag gacgagtgga agatcttcga
    14881 tccgcgcagc gcgcgcgccg gcaagcctgc cgacttcatg atctggcgtc gcggcgatgt
    14941 ctcgctgctg gatgtcgagg gcgggcgcga gtcgcaggtg accttctcga tcttgcgcag
    15001 ctaccgtccc gcgcgcgaca tcgcgctggc caacgccgtc gacaaccgcg cggcgctgat
    15061 cgacttctcg atctactcgc tgccgatcga ggaacagaat gccttcaaga ccatcctgct
    15121 ggtgccgatc ggcacgctgg tggtggcgat cttccgcatc ctgatcggcc tgcgcacctc
    15181 gggcaccttc atgccgatcc tgatcgcgct ggccttcatc cagaccacgt tgctgtgggg
    15241 cctgctgctg ttcctgatcg tggtcggcgc cggtctgttc atccgcttcc acctggcacg
    15301 gttgaacctg ctgctgatcg gacggatatc gtccgtggtc acggtggtca tcggcctgat
    15361 gtcgggcatc agcatcctca gctacaagct cgggctgaac cagatcctca cgatcacctt
    15421 cttcccgatg atcatcctgt cgtggaccat cgagcgcatg tcgatcctct gggaagagga
    15481 aggcccgaag gaagtcgcga tccagggcgg cggcagcctg ctggtggcca cggcagccta
    15541 cctgctgatg agcaatgcgc tggtcgcgca cctgaccttc aacttccccg agctgctgct
    15601 ggtgcagctg gcgctgatca tgctgatcgg ccagtacacg ggctaccgcc tgaccgagct
    15661 gaagcgcttc accccgctgg tggagaaccg ctgagatgcg attcgccaac ccgttcgcca
    15721 accccaggcg cctgcgccag gtcgggctgc tgggaatgaa cgagcgcaac tggttcatct
    15781 cgcgctacaa ccagcgcaag ctctacccgc tggtagacga caagctgaag accaagctgc
    15841 tcgccgagca acgcgggctg gcggtaccga agctctacgg cgtggtacgc gagcagcacg
    15901 aagtggagac catgggcgaa ctgctcgaca agtacgacag cttcgtgatc aagccgagcc
    15961 gcggcagcgg cggcaagggc atcaaggtca ttgtcggcaa gcgcgacgac gtgttcatca
    16021 agtcgagcgg cgacgaggtc acgcacctcg acgtcacgcg ccacgtctcc aacatcctga
    16081 gcggcctcta cagccttggc ggcaagcccg acgtggcgat gatcgaggcg cttgtcgaga
    16141 tggacccgca gttcaacggg ctcagctacg agggcatccc ggatatccgc atcatcgtgt
    16201 tcatgggcta cccggtgatg tcgatgatgc gcctggccac gcacgcctcg gacggcaagg
    16261 ccaacctgca ccagggcgcg gtcggcgtgg ggctcgacct cgcgacgggg cgctcgctgt
    16321 tcgcggtgca gtacaaccgc ccgctcgaac tgcacccgga caccggcctg ccgctgaaca
    16381 cgctcacgat cccccactgg cacgaactga tgtgcctcgg cgcgagttgc cacgagatca
    16441 ccggcctggg ctatctcggc gccgacatcg tgatggaccg caaccgcggc ccgctgatca
    16501 tggaactgaa cgcgcgcccg gggctcgcga tccagatcgc caacaacacc gggttgtcgc
    16561 cgcgcatccg ccgcatcgcg cgcgaggcgg cggcgaaccc gcagcgcccg gtcgaggagc
    16621 gcgtgcaatt cagcatgagt accttcggca cggaacccgc gctggccaaa gcggtctgag
    16681 cgatcaggcg gtgcgcgttt gcgccaccgc agcaatcgtc atcacccacg ccgtcgtgga
    16741 ggctcccggt atgaaccgcc atctgtgcag tgcactgacc gtggcgtgcc tgcaacttcc
    16801 ggtggccacg ctgctcgcgc aggaggctgc gcccggacag gttgccgggg gcggtggcct
    16861 gaactgcatt cccctgaagc gcatccagaa cacccgggtg ctcgatgacc ggacgatcct
    16921 gttcgaaatg accggcaacg agaccctggt caatcgcctg ccgcaccgct gtcccgggct
    16981 ggggttcgaa aagagtttcg gttacaagac cagcatcagc cagctgtgca gccaggacat
    17041 catctgggtg gtcacccaca tgggctcggg actggatcgc ggcgcgagct gcggactcgg
    17101 caggttcgag ccttatgtgg cgcctgaaac cgacgcgtcg gacaagggca agctggagcc
    17161 gatgtag
//
LOCUS       DAORUQ010000056        17152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3029_length_17152_cov_6.050594, whole genome shotgun sequence.
ACCESSION   DAORUQ010000056 DAORUQ010000000
VERSION     DAORUQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_17152_cov_6.050594"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLW13_06880"
     CDS             complement(<1..713)
                     /locus_tag="PLW13_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQY70133.1"
                     /translation="MPVTIHVQFMSPEGAHDRIFGPAKSVVHDGVAQVVRDAVGADAR
                     ICVGYNNHSSYITTRQFMTAYNNLGIMEVLFKAQEDGADVALIACGNDPALLMTREAL
                     DIPVLGITECAMLLACSLGKRFAAVGVERGCADIVESNLEAYGLADKAIRRNPVRTAD
                     LEESLIPWFQDPELVRTHVIPRFEEAARGAIEDGAEVIVTSCAGFAVLSKAGYHRVGG
                     TEVPVIEGVLAGAYLARVYG"
     gene            854..1828
                     /locus_tag="PLW13_06885"
     CDS             854..1828
                     /locus_tag="PLW13_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY70134.1"
                     /translation="MKHIDIRRIDLNLLIAFEAIYQEGSVTGASERLYLTQSALSHAL
                     SRLRELCDDPLFERHGKIMVPTGMARQLIVPVQSALTLLERSLNQPYPAVSGKVRPRL
                     TIGLISMYEAAFLPRLMARMAGEPDYEIAVTRYAPGKLEGHLAQGKFDVAIQMETPHS
                     VHVHSTPLLRERLAVMARRGHPQAGEDIDLATYMAQYHVVVVPDERCTDFVSQEFQRL
                     RLNRQVVLRCQDYWTAAQTVASSDFLLTAQRAALEQVLQSFPGNQLLPMPSDLDTPEA
                     MEVRLYWHASRDEDPANRWLRQQLRAIFRKCSAAPRLSRAHPGGSGSA"
     gene            complement(2000..2140)
                     /locus_tag="PLW13_06890"
     CDS             complement(2000..2140)
                     /locus_tag="PLW13_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70135.1"
                     /translation="METWRQGGDSLAGRYLLFRLHPISVREWCEQQGATPAAALERPR
                     PR"
     gene            2188..2463
                     /locus_tag="PLW13_06895"
     CDS             2188..2463
                     /locus_tag="PLW13_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70136.1"
                     /translation="MALQHGAIRRDVPVEALRRIEPLHELAAQRGLADLPRVGDQHHL
                     ALQILREKVIEIKSHGDFFPVCGELVAFWLCCQLAAALEARQPGAAY"
     gene            2631..4895
                     /locus_tag="PLW13_06900"
     CDS             2631..4895
                     /locus_tag="PLW13_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70137.1"
                     /translation="MLAAICLGAEALAQPPGPASDSLRLEEMTVTARRQEESLQQTPV
                     AVTALSGNLLDKLNVQDVSKLGQLAPNLVITQQTSSLNAAAIYIRGIGQNEPAANAEA
                     GVGIYLDGVYVARTAGAIFDLVDVERIEVLRGPQGTLFGRNTTGGAVQLVSRKPGDEF
                     GVEAKAGYGSYDDWYTRARIDTGQLGSSTIKATLAYLHRERDGYFDNELASDEEDPGS
                     LGSDSVWLGVRGDLGDSFSADFVLDYSEREGAPVFFQMVAANPDVRKFYGQSPSYGGA
                     PFVVTGTRLDDGQQAPFDDRFKSDSEVFGSALTLQYDLSEALAIKSISAYRYFNQDTI
                     CSLTGSGVMRGFVLDPVSYAPVGIEDLNGPYNCNNAPQRQDQYSQELQLVGATERWKY
                     VGGVFYFNETADEYNSQRLTFVLPGGTAGLNLAPVSAFGGETTSRAIFGQASYRPLML
                     DDRLEVSVGGRYTKDEKTFFSSAYAEEGDQDFDNTSWLLSFNYQLSDDLMGYARVSTG
                     YKAGGFSPRSAVLSSFEPEEATAYEAGLKSEWFDKRVRANMALFHTVYDDLQISQFLA
                     DSSGSSAIIVNAGEATFQGVEVEVAALVSENLTLTASWGYTDPTYDEYLYRDPQTNEL
                     IDVSDEARFVQMVRNNWHLSADYVFPPLSFGELSARLDYAQSSERYMFPLDRETPFNE
                     EIHDPGQENLSARLTLADMRVGDEGTWEIGVWGDNLTDQNNVGDGIDFGGLGFAGKYW
                     LQPRRFGVDVKLKL"
     gene            4995..7370
                     /locus_tag="PLW13_06905"
     CDS             4995..7370
                     /locus_tag="PLW13_06905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HQY70138.1"
                     /translation="MALKHFLLWSLGGLLAIVGIVLGIGGAWLLGLGGSAYYLPAGLA
                     HLVAGVLIGRGRVLGVWIYCAVFLATLAWALAEVGLDFWLLLPRIGLPLILVLYMFTP
                     WMRTHLGLRASIPADASSQRAGRAVVIAAAGVVALVTAGLLVQGGKENAQPPARGTAS
                     RPATDWANYAGNKAGTRFSPASQITRENVSGLDVAWTYRTGDLPENHPNSRAPQMFQA
                     TPLQIGDTLYLCTPRNIVVALDVDTGKERWRYDPGVDETGVHTLACRGVSYHRSQPPQ
                     AADCAGRILVATVDGRMIALDAQTGRPCRGFGRDGEISLRIGLGHVRGGFHTVTSPAI
                     VVGDVAVVGSFVLDNMTVDAPSGVVRAFDVISGKQLWNWDAGRQDPNAELKPGESYVP
                     GSPNAWSLFSADEQLGLVYIPTGNPSPDYFGGLRSPELERFGSSVVALDVPTGKVRWS
                     FQTVHHDLWDYDVASQPVLVDMPVDGELVPALIQATKQGEIFVLDRRNGSPIGPIEER
                     PVPRGDVPGERYSPTQPVSLALPSLAPPVMREADMWGATPLDQLWCRIEFRTLRYLGR
                     FTPPSLENSLIFPGNNGIMNWGSVAVDSARGILVVPTSYMPMTLQLVPRERAPATDQI
                     VLEGRAAVSPMLGTPYAVRTERPFASPLGLPCNAPPWGKLTAMDLATRQILWQRPLGT
                     TADHAPLRIPVPGVFNQGGPLVTQGGVVFIGAAMDNYLRAFDLESGTELWKGRLPAGG
                     QALPMSYVSPRTGRQYVVIAAGGHQFMSTTIGDHVVAYSLKSGAPLQQRAD"
     gene            7374..8396
                     /locus_tag="PLW13_06910"
     CDS             7374..8396
                     /locus_tag="PLW13_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQY70139.1"
                     /translation="MRAAIFEEAGRPLNVVTVEDPRPAPDQVVIEVAHCGICGSDLHV
                     TQQGLVPSGTILGHEFAGTIVEIGREVGGDWRVGDRVTALPIQACRSCEPCAIGLPGL
                     CSSVLFTGTTLETPGAYAQYVRARRTMLQRLPAGVSFAEGALVEPLAVAHHAVGLAGE
                     LSGASVLVIGAGPIGVGVALFARLAGARHVVVSEPAHGRRQTALGMGATHAIDPAAGP
                     VARSFATLAGRQPGVVFECVGNPGMLQHAIELAGVRGRVLVAGVCLEEDRIRPLTALG
                     REVSLQFSQCYTERDFSSVIDAIAHGRADATPMLTRIVGFDELPSAFEALRQPGEQCK
                     LLIDPH"
     gene            8593..9378
                     /locus_tag="PLW13_06915"
     CDS             8593..9378
                     /locus_tag="PLW13_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY70140.1"
                     /translation="METIRQIAGAGLGHYSSVFSAAELLAVLYYRTLRLDPSNPQWPG
                     RDKFLLGKGHIGVGLWPILADLGYFPAAWLDDFGQMDAPLTDHPVMRQVPGVDFSSGS
                     LGHNLSVGVGMCLASRFRGGDDRTFVLLGDGELHEGQVWEAAMAASHYKLGNLVAIVD
                     ANGSSADGPTSDVMNIEPVAGRFEAFGWRAIEMDGHDVSRIVEVLDDLPDARTAVPTV
                     IVARTLKGKGVSFMEAAPREWHLGFLGPDDRQRAENEIRSRMR"
     gene            9375..10367
                     /locus_tag="PLW13_06920"
     CDS             9375..10367
                     /locus_tag="PLW13_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQY70141.1"
                     /translation="MSTSTIEMVDPFSFSSAAEVEGGAVIGQTLIELARERDDIVLIA
                     PDVGRGPATTAFIEAFPDRYIDTGIAETNSVSIAAGLAAAGYRPFVFGIGAFLAPKCM
                     EQIRTDIAVNRLPVTMISAWGGLDMGFFGASHHGIEDISILRGTPHLTIASAADDVSM
                     RALMRTAVTEGLPFYIRAPSAGAADVYDTAPRVARGELSMVSQGVDVALVGTGLGTSL
                     CVQAAAVLQESGVSATVVDAVYLKPFDGEAICRLARTVRAVVVVEEHNVRAGLTSLVA
                     EALGRGGVAVPLEGVGLPDGDLAVAVPQRLLARYGLTADAIAAKAIAALGRSRS"
     gene            10421..11503
                     /locus_tag="PLW13_06925"
     CDS             10421..11503
                     /locus_tag="PLW13_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY70142.1"
                     /translation="MNRRSARSAPRPPTGLLLQQVLRSVRHVGGDEDAILRRAGIAGL
                     GEKAGRDGWMQLISHDQFVSIYRECVDLLERSDAKCSGRPAMGVSEFHMLCYCMISSA
                     TLGQAIDRAAAFGRLFEGGVGEFTLRTVRENAEFRMHSASRPRDAHMIFGVLTGLSSF
                     ARLFGWLVGKDLSPLTVKICYGPVLDEHVVSWLLPWRVEYEAEDNLLCFPARYLALPL
                     VRSAAELDELLTTFPFDLATGRSSVTPASVWVRKICATALAHCSAPPNTEQIARQLGL
                     SVATLKRRLSDENTSVRELKERCRCELAYDLLGDRSLTLREISLRLGFSDTTAFSRAF
                     KAWTGRVPSAIRHESGLVGLNRRETR"
     gene            complement(11654..11755)
                     /locus_tag="PLW13_06930"
                     /pseudo
     CDS             complement(11654..11755)
                     /locus_tag="PLW13_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014719083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(11948..13393)
                     /locus_tag="PLW13_06935"
     CDS             complement(11948..13393)
                     /locus_tag="PLW13_06935"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY70143.1"
                     /translation="MQRIAIIGAGPGGICAGIHLKQAGHEDFVILEQAGGVGGTWWHN
                     SYPGCACDVPSHLYSFSFALKRDWPRPYATQPEIRAYMEDCVARFGLWPHIRLKTPVS
                     AARWIETQAAWELTLADGGTLQAEVLVGAVGMFNLPAWPAIAGLDRFAGTVFHSARWN
                     HAHDLAGRDVAVIGSAASAVQFVPQIARLARRLDVYQRTPNWVRPREDAYTPEQLAEF
                     RDDPAAAAAERETVWNWVNAIQTLVDPDILRDSAAVCLQNLDVVKDPATRAKLTPDYP
                     FGAKRALISNDWYPTFNRSNVQLVSEPIAGITPEAIVTRDGTPRVVDTIVLATGFDTT
                     RFLSTIDVSGRDGVRLDDAWADGAQAYLGITLAGFPNLFMLYGPNTNNGSIIFQIECQ
                     VDYLLRHLQRMTREKLAWIEVKPEVMADYNRRLQADLDAVAVWHSGAHDYYRAASGRI
                     VTQWPHGMDRYREITAANDDDAYLGGGDTGD"
     gene            complement(13438..14271)
                     /locus_tag="PLW13_06940"
     CDS             complement(13438..14271)
                     /locus_tag="PLW13_06940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70144.1"
                     /translation="MDLGLAGKTAIITGGSGGIGRGLVLEFAREGCNVISASRDDATG
                     RKLAEEARTLGLKGRVLAVKTDVTRRDSVDAMVAEARREFGPVDVLVNNAGGVAHPSD
                     FEKFDEEGRRWEIALNIDGVVNCCQAVAEDMLGRKHGSIINISSNSSLLGEAAANIVH
                     YGGVKGFVNSFSKALAWEWAKKGVRVNNICPGWIVPYKAEEVGGGSFWNRFGFDTMGK
                     PDDMEKALEEGTLYNMSGLPIPRLGRPEDIANLALFLASDLSGYITGQLISVSGGAYM
                     P"
     gene            complement(14305..15078)
                     /locus_tag="PLW13_06945"
     CDS             complement(14305..15078)
                     /locus_tag="PLW13_06945"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70145.1"
                     /translation="MELGLQDKVVIITGAASGIGRASLRAFAAEGARVLAVDINAAQL
                     AAAVEELPAARVKAHAADVTDADQVQTMVAHAVAAFGRLDVMFSNAGGAIPTPTEQQG
                     LADYRRIMALNLDAVYYAVHAALPVMLAQGSGCFLSTSSGAGMNAATGLAVYGAAKAG
                     VISLMRSIATEYGGRGIRANTIAPGPMDTPPVRAWLGTFDNGLARFARQVPSGRLGTG
                     EDIARAAVFLASDAAEFINGAAVPVDGAVHARLASPQID"
     gene            complement(15104..16294)
                     /locus_tag="PLW13_06950"
     CDS             complement(15104..16294)
                     /locus_tag="PLW13_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQY70146.1"
                     /translation="MTADTTNEDALLSAAWDDFCEQLKSAGRLPFRPQVPTGDLSRAE
                     GFRTLAANIGLALQFHLENNDPLHPELIHYFDPIRKQGGDNPDSVYVGGPINGTDTYR
                     ISGHRGTARYFAVTAVETGNTPWGGKVAHTLFGGDIHVEADGSFELICSPHPQPGNWL
                     RTTPDTFRVTFRQFFADWETETPMQARIERLGAAAPPPVVDAPRLAEGLGVAARWVGE
                     SVEYWANMIEMWKARPNTFLSYRQLDSNALDATPGGEPMIAYWSVPADEALIVRVKPP
                     RAGYWAVEFGNYWWSSMDYRNRLCGTNCHYAALEDDGELILVVSHVDPGVANWIDPSG
                     FAEGYMTIRWMLADHYPQPQATQVKLADLERHLPATTKRVTPAQRAGQIAARRRGVLQ
                     RWGH"
     gene            complement(16291..16893)
                     /locus_tag="PLW13_06955"
     CDS             complement(16291..16893)
                     /locus_tag="PLW13_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70147.1"
                     /translation="MPRIVDHAERRASIARSAALAIAELGLEQATMREIAARSGVSKG
                     IVEHYFANRDEVIETALEWANARYVARETRRTRGLQGMDALRERLRCVLPLDAESRQD
                     WKIRLCVWSVAAVNAGARKAQRARLDRTRERFVTDLQAARALGQAPAGIDPVATANEL
                     MHLVAGLACDALLDPRYYDRPYLLGLIDTTINRLTGRSQP"
BASE COUNT         2720 a         5518 c         5878 g         3036 t
ORIGIN      
        1 ccgtaaacgc gcgccaggta cgccccggcc agcacgcctt cgatcaccgg cacctccgtt
       61 ccgccgaccc tatgatagcc ggccttgctg agcaccgcga agccagcgca ggaggtgacg
      121 atgacctcgg cgccgtcctc gatggcgccg cgcgcggcct cctcgaagcg cgggatcacg
      181 tgcgtgcgca cgagctcggg gtcctggaac cagggaatca ggctctcctc gaggtccgcg
      241 gtgcgcaccg ggttgcgcct gatggccttg tcggcgaggc cgtaggcctc caggttgctc
      301 tcgacgatgt cggcgcaacc gcgttcgacg ccgacggccg cgaagcgctt gcccagcgag
      361 caggccagca gcatggcgca ttccgtgata cccagcactg ggatgtccag tgcctcgcgt
      421 gtcatgagca gggcgggatc gttgccgcag gcgatcagcg ccacgtcggc gccgtcttcc
      481 tgcgccttga acaggacctc catgatgccg aggttgttgt aggccgtcat gaactgccgc
      541 gtcgtgatgt aggaggagtg gttgttgtag ccgacacaga tgcgcgcatc ggcgccaacg
      601 gcgtctcgca cgacctgcgc caccccgtcg tgcacgacgg acttggcggg gccgaagatc
      661 cggtcgtgcg cgccctcggg cgacatgaac tggacgtgta tggtgacagg cataacgggg
      721 tatcctcttt cttgttttat cggcaggggg cgcgcggctg gccgccggaa cagtggtctg
      781 cccggaaagc ccggatgcgg gaatctaatc gccaggcgcg cgccggctcc agtgaaagcg
      841 ctgcatgagg gccatgaagc acatcgatat acgtcgcatc gacctgaacc tgctgatcgc
      901 cttcgaggcg atctaccagg aaggatcggt caccggcgcc agcgaacggc tgtacctcac
      961 gcagtctgcg ctcagccatg cgctgtcgcg gctgcgcgag ttgtgcgacg accccttgtt
     1021 cgaacgccat ggcaagatca tggtcccgac gggcatggcg cggcagctga tcgtgccggt
     1081 gcaatcggca ctgacgctgc tggaacgatc cctgaaccag ccgtacccgg cggtgagcgg
     1141 caaggtccgg ccgcgcctga ccatcgggtt gatctcgatg tacgaggcgg cattcctgcc
     1201 gaggctcatg gcccggatgg caggcgagcc cgactacgag atcgccgtca cgcgctacgc
     1261 gccgggcaag ctcgaggggc acctcgcgca ggggaagttc gacgtggcga tacagatgga
     1321 gactccccac tccgtccatg tgcacagcac accgctgctt cgcgagcgac tggcggtcat
     1381 ggcgcggcgc ggtcacccgc aggcgggtga ggatatcgat ctcgcaacgt acatggccca
     1441 gtaccacgtg gtcgtggttc ccgacgagcg ctgtaccgac ttcgtcagcc aggagttcca
     1501 gcgtctgcgc ctgaatcgcc aggtcgtgtt gcgctgccag gattactgga cagccgccca
     1561 gacggtggca agttccgatt tcctgctgac ggcgcagcgg gccgcgctcg agcaggtcct
     1621 gcaatccttt cccggcaacc agctgctgcc catgccctcg gacctcgata cgccggaggc
     1681 gatggaggtg cgcctgtact ggcacgcgtc ccgggatgag gatcctgcaa accgctggct
     1741 gcggcagcag ctgcgcgcga ttttccgcaa atgtagtgca gccccgcggc tttcccgcgc
     1801 gcatcctggc ggaagcgggt ctgcttgagc agcatggtgg ccaccgcatt ctcgaagcgc
     1861 acgccttcgt cgccgcgcac gagcccggtg tcgaagaaat agaccttggg cgtctgcagg
     1921 atcgcgcggc cgatgttgtg gtgccacggc tgtaccgtga acacgatgta gagcgcctac
     1981 agtatgtcga ggtagcgctt cagcgtggtc ggggacgctc gagagcggcg gcgggcgtcg
     2041 cgccctgctg ctcgcaccac tcgcgcacgg aaatggggtg caacctgaac agcaggtaac
     2101 ggccggccag cgaatcaccg ccctggcgcc aggtttccat gcgggcgctg cccgtgacga
     2161 ggagcgcctg gtgcgcgggc tccagccttg gcgctgcagc acggcgctat ccgccgcgac
     2221 gtcccagttg aggcattgcg acggattgaa ccgctccatg aacttgcggc acagcgtggc
     2281 cttgccgacc tgccgcgcgt cggtgatcag caccatcttg cgctgcagat ccttcgtgag
     2341 aaggtcatcg agataaagtc tcatggcgac ttttttccag tttgcggtga attggtcgcc
     2401 ttttggttgt gctgtcagct cgcggccgcg ctcgaggcac ggcagccggg cgcggcatat
     2461 tgagctgaat ccgtcggtac tttgagccgt tctgtcgttg tcgtgagccg ccgccgcgtc
     2521 cagcattacg atgccttgcg tgcacgctaa atataaacag caaacctccc ggagacttgc
     2581 tcccgatgct caagccatgc actcgttcga agcttgccgg cagcgccacg atgctcgctg
     2641 ccatctgcct cggcgccgaa gcccttgcgc agccgcccgg gcctgcgtcg gactccttgc
     2701 ggctcgagga aatgacggta acggcgcgcc gtcaggagga atccctgcag cagactccgg
     2761 tcgcggtgac cgccctgtcg ggcaacctgc tcgacaagct gaacgtgcag gacgtgagca
     2821 agctcggaca gctagcgccg aacctcgtca ttacgcagca gaccagcagc ctgaacgcag
     2881 cggccatcta tatccggggc attgggcaga acgagcctgc ggccaatgcc gaggctggtg
     2941 tcggaatcta tctggacggc gtctacgtcg cgcggacggc cggtgccata ttcgatctcg
     3001 tcgatgtcga gcgcatcgag gtcctgcgtg gcccgcaggg gacgctgttc ggacggaaca
     3061 cgactggtgg agctgtacag ctggtcagca ggaagccggg ggacgagttt ggcgtggagg
     3121 caaaggccgg ctacggcagc tacgacgact ggtacacgcg cgcgcggatc gacacgggcc
     3181 agctcggcag ttccacgatc aaggcgacgc tcgcctacct gcaccgcgag cgtgacgggt
     3241 atttcgacaa cgagctcgcg agcgacgagg aggatccggg ctcccttggc agcgactccg
     3301 tctggctggg cgtgcgcggt gatctcggcg acagcttttc tgccgatttc gtgctcgact
     3361 acagcgagcg cgaaggcgcg ccggtatttt tccagatggt cgccgcgaat ccggacgtcc
     3421 gcaaattcta cggccagtcg cccagctacg gcggggctcc cttcgtggtc accgggacgc
     3481 gactcgacga cggccagcag gcgcccttcg acgaccgctt caaaagcgac agcgaagtgt
     3541 ttggcagcgc gctcacgctt cagtacgacc tcagcgaagc gctggcgata aagtcgatct
     3601 ccgcttaccg ctatttcaac caggacacga tttgcagcct caccgggagc ggcgtgatgc
     3661 gcggcttcgt gctcgacccg gtcagctacg cgccggtggg catagaagat ctcaatgggc
     3721 catacaactg caacaacgcc ccgcagcgac aggaccagta ttcgcaggaa ttgcagctgg
     3781 tgggcgccac ggagcgctgg aagtatgtcg gcggcgtttt ctatttcaac gagacggccg
     3841 acgagtacaa cagccaacgc ctcacgttcg tgcttcccgg cggtacggcg gggctcaacc
     3901 tcgcccctgt ctcggccttc ggcggcgaga cgacctcgcg ggcgatcttc ggccaggcga
     3961 gctaccggcc gctgatgctg gacgacaggc tcgaggtctc ggtgggcggg cgctacacga
     4021 aggacgagaa gaccttcttc tcatccgcgt atgcggagga aggagaccag gatttcgaca
     4081 atacgtcctg gctgctctcg ttcaactacc agctctcgga tgacctgatg ggatatgcgc
     4141 gtgtgtcgac cggctacaag gcgggcggct tctcgccgcg ctccgcggtg ctgtcgagct
     4201 tcgagcccga ggaggcgacg gcctacgagg cggggctcaa gagcgagtgg ttcgacaagc
     4261 gcgtgcgcgc gaacatggcg ctgttccaca cggtctacga cgatctgcag atttcgcagt
     4321 tcctggcgga ttcctcggga tcgagcgcca tcatcgtcaa cgcgggtgag gcgaccttcc
     4381 agggcgtcga ggtggaagtc gccgcactgg tgagcgagaa cctgacgctg accgcgtcgt
     4441 ggggatacac cgacccgacc tacgacgaat acctgtaccg cgacccgcag acgaatgagc
     4501 tgatcgacgt atcggacgaa gcgcgattcg tgcagatggt gcggaacaat tggcacctga
     4561 gtgccgacta cgtgttcccg ccgctgtcct tcggtgaact gagcgcccgc ctggactatg
     4621 cgcagagttc ggagcgctac atgtttccgc tcgatcggga gacgcccttc aacgaggaga
     4681 tccacgaccc cggtcaagag aacctgagcg cgcgacttac cctggccgac atgcgtgtcg
     4741 gcgacgaggg tacctgggaa ataggcgtat ggggcgacaa cctgacggat cagaacaacg
     4801 tgggtgacgg catcgacttc ggcggcctgg gattcgcggg caagtactgg ctccaaccgc
     4861 gtcgctttgg tgtcgacgtc aagctcaagc tctgaacgtt gccgatcccc gcagacggga
     4921 cctaaaggcg agtcaggcgg tccaggtctg cgccggggat caactgccct gaaagggatg
     4981 cggagcatat gagcatggcc ctcaagcatt tcttgctctg gtcactgggt ggcctgctcg
     5041 cgatcgtcgg catcgtgctg gggatagggg gcgcctggct gctgggtctc ggcggatcgg
     5101 cctattacct gcctgcgggc ctggcgcacc tggtcgctgg cgtcctgatc ggccgcggca
     5161 gggtgctggg cgtctggatc tactgcgccg tttttctggc gacgctcgct tgggccctcg
     5221 ccgaggttgg cctcgatttc tggctgttgc tgccgcgcat cggtctaccg ctgatcctgg
     5281 tgctctacat gttcaccccg tggatgcgca cgcatctcgg gctgcgggcg tcgatcccgg
     5341 cggatgcctc gagccagcgg gccggacggg ctgtcgtaat cgctgcggca ggcgttgtgg
     5401 cgctcgtgac cgcgggtttg ctggtccagg gggggaagga aaacgcgcag ccgcccgcgc
     5461 gtggcacggc atcccggcct gcaaccgact gggccaacta cgcggggaac aaggccggca
     5521 cccggttttc gccggcatca cagatcacac gggaaaacgt atccggactg gatgtcgcct
     5581 ggacgtaccg gaccggagat cttccggaaa atcacccgaa cagccgtgcc ccgcagatgt
     5641 tccaggcgac gccgctccag atcggcgaca cgctttacct gtgcacaccg cgaaatatcg
     5701 tcgttgccct cgatgtggac accggcaagg agcggtggcg atacgatccc ggggtcgacg
     5761 agacgggcgt ccataccctt gcctgccggg gcgtctccta tcaccgctcg caacccccgc
     5821 aagcggcgga ttgcgcaggg cgcattctgg ttgccacggt cgatggccgg atgatcgcgc
     5881 tggatgcgca gacggggcgt ccttgccggg gcttcggtcg cgacggggag atctcgcttc
     5941 gcatcggtct gggccacgtg cggggcggct tccataccgt cacatcgcct gcgatcgttg
     6001 tcggcgatgt ggcggtcgtc gggagtttcg tgctcgataa catgacggtc gatgcaccgt
     6061 cgggcgtcgt ccgggcattc gacgtcatca gcggcaagca actgtggaac tgggatgccg
     6121 ggcggcagga tcccaatgcc gaattgaagc ccggcgaaag ctacgtgccc ggatcgccga
     6181 acgcgtggag cctgttcagc gcggatgaac aactgggcct cgtgtacatc ccgacgggca
     6241 acccgtcgcc ggactatttc ggcgggctgc gaagcccgga gttggagcgt ttcggcagtt
     6301 cggtcgtcgc gctcgatgtt cctaccggca aggtgcgctg gtcattccag acggtgcatc
     6361 acgatctctg ggattacgac gtggcgtccc agccggtgct cgtcgacatg cccgtggacg
     6421 gagagcttgt tccggcattg atccaggcga ccaagcaggg tgagattttc gtgctggacc
     6481 gcaggaacgg tagccccatc gggccgatcg aggagcggcc tgttccgcgc ggggacgttc
     6541 cgggcgagcg ctattccccc acgcagccgg tttccctcgc gctgccgagc ctcgcgccgc
     6601 cggtgatgcg cgaagcggat atgtgggggg cgacgccgct ggaccagctc tggtgccgga
     6661 tcgagttccg cacgctgcgc tatctgggac gcttcacgcc gccatcgctc gaaaacagcc
     6721 tgatctttcc cggtaacaac ggcatcatga actggggtag tgttgccgtg gattccgcgc
     6781 gcggcatcct cgtggtgcct acctcctata tgccgatgac gctgcagttg gtcccgcgag
     6841 agcgcgcgcc cgcgacagac cagatcgtgc tcgaaggacg tgctgccgtc tcgccgatgc
     6901 tgggcacgcc ctatgcggtt cgtaccgaac gcccattcgc gtcgccgctg ggcctgcctt
     6961 gcaatgctcc tccgtggggg aagctgaccg cgatggacct cgcgacccgc cagatcctct
     7021 ggcagaggcc gctcgggacc accgccgacc acgcgccttt gcgtattccg gttcccggtg
     7081 tgttcaacca gggcggtccc ctggtcacgc aaggcggcgt cgtcttcatc ggagcggcaa
     7141 tggacaacta tctacgcgcc tttgatctcg agagcggcac ggagttgtgg aaaggcaggc
     7201 ttcccgccgg tggccaggcc ctgcccatga gttacgtgtc gccgcgcacg ggcaggcagt
     7261 atgtggtgat cgccgcgggc gggcaccagt tcatgagcac gaccatcggc gatcacgtgg
     7321 tcgcctacag cctgaagtca ggcgcccccc tgcagcagcg agcggactga gccatgcgcg
     7381 cggcgatatt cgaagaggcg ggacgccccc tgaacgtggt tacggtcgag gatccgcggc
     7441 cggcgcccga ccaggtggtg atcgaggtcg cgcattgcgg catttgcggc tccgatctgc
     7501 atgtaacgca acagggcctg gtgccatcgg gaaccatcct ggggcatgag ttcgccggca
     7561 ccatcgtcga gatcgggcgc gaggttggtg gcgattggcg ggtgggcgac cgggtaacgg
     7621 cgctgccgat ccaggcatgc cggagctgcg agccctgcgc gatcgggttg cccggcctgt
     7681 gcagcagcgt cctgttcacc ggcacgacgc tggaaacccc cggtgcatat gcgcagtacg
     7741 tgcgggcgcg gcggacgatg ctgcagcgtc tgccggccgg ggtctccttt gccgaggggg
     7801 cactggtgga gccgctcgcc gttgcccatc acgcggtagg cctggcgggc gagttgagcg
     7861 gagcctcggt gctggtgatc ggcgccgggc cgatcggcgt cggggtcgcg ctgttcgcgc
     7921 ggctggcggg cgcgcgtcac gtggtggtca gcgagcctgc gcacggtcgc cggcaaacgg
     7981 cgctcgggat gggcgccacg catgcgatcg atccggccgc cggccctgtc gcccggagct
     8041 ttgccacgct ggcgggccgc cagccgggtg tggtgttcga atgcgtcggc aatccgggaa
     8101 tgttgcagca tgcgatcgaa ttggccggtg tgcgcggccg cgtgctcgtg gccggtgtgt
     8161 gcctcgagga ggaccgcatc cgaccgctca ctgccctggg cagggaagtc agcctgcagt
     8221 tctcacaatg ctacacagag cgcgactttt cgagcgtaat cgatgcaatc gcgcacggaa
     8281 gggcggatgc caccccgatg ctcacccgga tcgtcggctt cgatgaattg ccgtctgcct
     8341 tcgaagcgct gcggcagccc ggcgagcaat gcaagttact catcgacccc cactgaggtc
     8401 tccgggagcg gaccgtgttc atccgggcct gctccacaga cagctccgcg ctacatgaaa
     8461 ggagaaaaca ctcatgcaca gacaattgga ggccacgtga tgtcctgtac cggcagtatt
     8521 cgacccccgc cagaattcct cacgcgaccc tgttcgatcg aggctctccg ctggcgagcg
     8581 gaatggatcc gcatggaaac catccggcag atcgccggcg ccgggctcgg ccactattcc
     8641 agcgtgttca gtgccgccga gctgctggcg gtcctctact accggacgct ccggctcgat
     8701 ccgtcgaatc cgcaatggcc gggtagggat aaattcctgc tcggcaaggg gcatatcggc
     8761 gtcggattgt ggccgatcct ggcggacctg ggctatttcc ccgcggcctg gctggatgat
     8821 ttcgggcaga tggatgcacc gctcaccgat catccggtca tgcgccaggt ccccggcgtg
     8881 gacttcagtt ccgggtcgct cggtcacaac ctgtcggttg gtgtgggcat gtgcctcgcg
     8941 tcgcgttttc gcggtggcga tgaccgcacg ttcgtgctgc tcggtgatgg cgagttgcac
     9001 gaggggcagg tctgggaggc ggcgatggcg gcgtcccatt acaagctcgg caacctcgtg
     9061 gcgatcgtcg acgccaatgg aagctcggca gatggaccga cctccgatgt catgaatatc
     9121 gagccggtgg ccggtcgttt cgaggcgttc ggatggcgcg ccatcgagat ggacggccat
     9181 gacgtgtccc ggatcgtcga ggtcctggac gatctgcccg acgcgagaac agccgtaccc
     9241 accgtgatcg tggcgcgcac gctcaagggc aagggggtat cgttcatgga ggcggcgcca
     9301 cgtgaatggc acctcggctt cctgggaccg gacgaccgcc aacgcgcgga aaacgaaata
     9361 cggagccgca tgagatgagc acttcaacca tagagatggt cgacccgttt tccttttcca
     9421 gcgccgcaga ggtcgaaggg ggagccgtca tcggccagac gctgatcgaa ctcgcgcgcg
     9481 agcgcgacga catcgtcctg attgccccgg acgtcggtcg tggccccgcg acgacggcct
     9541 tcatcgaggc atttccggat cggtatatcg ataccggcat cgcggagacc aattcggtgt
     9601 cgatcgcggc gggactcgcg gccgcgggtt atcgtccgtt cgtcttcggc atcggcgcgt
     9661 tcctggcgcc caagtgcatg gagcagatcc gcaccgatat tgccgtcaac cggttgccgg
     9721 tgacgatgat ctccgcctgg ggcggtctgg acatggggtt tttcggcgcg agtcatcacg
     9781 ggatcgagga tatctccatc ctgaggggga cgccgcatct gacgatcgcc tcggcagcgg
     9841 acgatgtctc gatgcgcgcg ctcatgcgca cggccgtgac cgagggcctg cccttctaca
     9901 tccgcgcgcc gagcgcaggc gcggccgatg tctacgacac tgcgccacgt gttgcgcgag
     9961 gcgaattgag catggtttcc caaggcgtgg acgtggcgct tgtcgggaca gggctcggga
    10021 cctcgttgtg cgtgcaggcc gcagccgtcc tgcaggagtc gggcgtgtcc gcgacggtgg
    10081 tggacgcggt ctatctgaag cccttcgacg gcgaggccat ctgccggttg gcccgaaccg
    10141 ttcgtgccgt cgtcgtcgtg gaagagcaca acgtccgcgc ggggctgaca tcgctggtgg
    10201 cggaggcgct gggtcgcggc ggcgtagcgg taccattgga gggcgtgggg ctgcctgatg
    10261 gcgatctggc ggttgccgtg ccgcagcggt tgctggcccg ctacggtttg accgccgatg
    10321 cgatcgcggc gaaggccatc gcagcgctcg ggcggtcgcg ttcataggag cgcaggcggc
    10381 tcggctacaa tgcgtggcat ccagcgacgg tatgcccccc atgaatcgac gttcagcccg
    10441 gtccgcgccc aggcctccca ccggcctgct gctgcagcaa gtactgcgca gcgtgcgtca
    10501 tgtcggcggc gatgaagacg cgatcctgcg ccgcgccggc attgcggggc tcggtgagaa
    10561 ggcaggccgt gacggctgga tgcagctgat ttcgcacgat cagttcgtat cgatctatcg
    10621 tgaatgcgtc gacctgctcg aacggtcgga cgcgaagtgc agcggccggc cggcgatggg
    10681 tgtgtccgaa tttcacatgc tgtgctattg catgataagt agcgcgacac tgggccaggc
    10741 gatcgatcgc gccgcggcct tcggccggtt gttcgagggc ggcgtcggcg aattcacgct
    10801 gcggacggtg cgggaaaatg ccgaattccg catgcactcc gcctccaggc cgcgtgatgc
    10861 ccacatgatc ttcggtgtcc tgacgggact gtcgagtttt gcgcgcctgt ttggctggtt
    10921 ggtcggcaag gatctgtcgc cgctcacagt gaagatctgc tacggtccgg tactggacga
    10981 gcacgtggtc tcctggctgc tgccctggcg tgtggaatac gaggccgaag acaaccttct
    11041 gtgctttcct gcgcgatacc tcgctctgcc actcgtgcgc agcgccgccg aactggatga
    11101 actgctcacc acgtttccgt tcgatctcgc gaccgggcga tcgagcgtga cgcctgcctc
    11161 cgtttgggtg cgcaagatct gcgccacagc actcgcgcac tgcagtgcgc cgcccaatac
    11221 ggagcagatc gcgcggcagc tgggattgag cgtcgcaaca ctgaaacggc gcctgagcga
    11281 tgaaaacacg tcggtcaggg aactgaagga gcgttgccgc tgcgagctcg cgtacgacct
    11341 ccttggcgac cggtcgttga cgctgcgcga gatctcgctt cggctcggct tcagcgatac
    11401 caccgcgttc tcgcgtgcct tcaaggcttg gacgggacgc gtgccctcgg ccatcaggca
    11461 cgagtcggga cttgtcggcc tcaaccgtcg cgagacacgc tgacaccgcc ccttgccacg
    11521 agcctgggtc ggcaagcaac ggcgcgatga atcgaatgcc gcggctacgg tccggcccgg
    11581 ctcgccagag agggaaatgc cggtttcatg tctcgcttgc ggtaacgctg acgagcggtg
    11641 cgcacccgcc ggttcagggc atgtaggctc cgccgctcac gctgatcagc tgcccggtga
    11701 tgtagccgga aaggtccgag gcgaggaaca gtgcaaggtt cgcgatgtcc tcggccacgg
    11761 cctggcagca gttcaccacg ccggtgtcag cctccctaca cgcgtgtaga aaaagcaggg
    11821 ccgcgagggg ccccgactga taacggatcg agcgcccggt tcagaactcg aaagtcacgt
    11881 ccaggccata ggtgagcggc tcgcgatgca agcgccggac acgacgcgtg tcctcggcgc
    11941 ctgcagatca gtcgccggta tcgccgccac ccaggtaggc atcgtcgtca ttggcggcgg
    12001 tgatttcgcg gtaacgatcc atgccgtgcg gccactgcgt gacgatgcgc ccggaggcgg
    12061 cgcggtagta gtcgtgcgcg ccgctgtgcc agacggccac cgcgtccaga tccgcctgca
    12121 ggcggcggtt gtagtcggcc atgacctcgg gcttcacctc gatccacgcg agcttctcgc
    12181 gcgtcatgcg ctgcaggtgg cgcagcaggt agtccacctg gcactcgatc tggaagatga
    12241 tcgagccgtt gttggtgttc ggcccgtaga gcatgaacag gttcgggaag ccggccaacg
    12301 tgatgcccag gtaggcctgg gcgccatcgg cccatgcgtc gtcgagcctc actccgtcac
    12361 gcccgctgac gtcaatggtc gaaaggaatc gcgtggtgtc gaagccggtc gccagcacga
    12421 tggtgtcgac gacgcgcggc gtcccgtcgc gcgtcacgat cgcctcgggc gtgatgccgg
    12481 cgatcggttc gctgacgagc tgcacattgg agcggttgaa ggtcgggtac cagtcgttgg
    12541 agatcagcgc gcgcttggcg ccgaaagggt agtcgggcgt gagcttggcg cgcgtcgcgg
    12601 gatccttcac cacgtccaga ttctgcaggc acaccgcggc ggaatcgcgc aggatgtccg
    12661 gatcgaccag cgtctgaatc gcgtttaccc agttccagac ggtctcgcgc tcggccgcgg
    12721 cggcagccgg atcgtcgcga aactccgcga gttgctccgg tgtgtacgcg tcctcgcgcg
    12781 gacgcaccca gttcggcgtg cgctggtaga cgtcgagacg ccgcgcgagg cgcgcgatct
    12841 gcggcacgaa ctgcacggcg ctcgcggcgc tgccgatcac cgcgacgtcg cgtccggcca
    12901 ggtcgtgcgc atggttccag cgcgccgagt ggaacaccgt cccggcgaag cgatcgagcc
    12961 ccgcaatggc cggccaggcg ggcaggttga acatcccgac cgcgccgacc agtacctcgg
    13021 cctgcagcgt cccgccgtcg gccaacgtga gctcccacgc ggcctgcgtc tcgatccagc
    13081 gcgccgcgct caccggggtc ttcaggcgga tgtgcggcca cagtccgaag cgcgcgacgc
    13141 agtcctccat gtaggcccgg atctccggct gcgtggcata ggggcgtggc cagtcgcgct
    13201 tgagcgcgaa ggaaaacgag tacaggtgcg acggcacgtc gcaggcgcag cccggatagc
    13261 tgttgtgcca ccaggtcccg ccgacgccgc cagcctgctc gaggatcacg aaatcctcgt
    13321 ggccggcttg cttcaggtgg ataccggcgc agatgccgcc gggtccggcg ccgatgatgg
    13381 cgatgcgttg cattcaatcg actcctcgat cgcgcacggg ccagccgacg cgccgcttca
    13441 gggcatgtag gctccgccgc tcacgctgat cagctgaccg gtgatgtagc cggaaaggtc
    13501 ggaggcgagg aacagcgcca ggttcgcgat gtcctcgggg cggccgaggc gagggatcgg
    13561 cagaccgctc atgttgtaca gtgtgccttc ctccagcgcc ttttccatgt cgtcgggctt
    13621 gcccatggtg tcgaagccga agcggttcca gaagctcccg ccgcccactt cctcggcctt
    13681 gtagggcacg atccagccgg ggcagatgtt gttcacgcgc acgcccttct tggcccactc
    13741 ccaagccagt gccttactga agctgttcac gaaacccttc acgccgccgt agtgcacgat
    13801 gttcgcggcg gcctcgccga gcagcgagga gttcgaggag atgttgatga tgctgccgtg
    13861 cttgcgcccg agcatgtcct cggccacggc ctggcagcag ttcaccacgc cgtcgatgtt
    13921 cagcgcgatt tcccagcgcc ggccttcctc gtcgaacttc tcgaaatccg aggggtgcgc
    13981 gacaccgccg gcgttgttca ccagcacatc gacgggaccg aattcacgcc gcgcctcggc
    14041 gaccatcgca tcgacgctgt cgcgccgtgt cacgtcggtc ttcaccgcga gtacgcgacc
    14101 cttcaggccc agcgtgcgtg cctcctcggc cagcttgcgc ccggtcgcgt cgtcgcgcga
    14161 ggcgctgatc acgttgcaac cctcgcgggc gaattccagc accagtccgc ggccgatgcc
    14221 gccgctgccg ccggtgatga ttgccgtttt gccggcgagt ccgagatcca tggcgtgctc
    14281 cctgtgtcag tggttcgtca ggcttcaatc tatctgaggc gaggcaaggc gcgcatgcac
    14341 cgcgccgtcc accggtaccg ccgcgccgtt gatgaattcg gcggcatcgc tggcaaggaa
    14401 caccgcggcg cgcgcgatgt cctcgccggt gccgagacgc ccgctcggga cctggcgggc
    14461 aaagcgcgcc aggccgttat cgaaggtgcc cagccaggcg cgtaccggcg gcgtgtccat
    14521 cgggcccggt gcgatcgtgt tggcgcggat gccgcggcca ccgtactcgg ttgcgatgct
    14581 gcgcatcagg ctgatcacgc cggccttggc ggcgccgtag accgccagcc cggtcgcggc
    14641 gttcatgccg gcgccggaag acgtgctcag gaaacagccg ctgccctgcg ccagcatcac
    14701 cggcagcgcc gcgtgcacgg cgtagtacac ggcgtcgagg ttgagcgcca tgatgcgccg
    14761 gtagtccgcg aggccctgct gctcggtggg cgtcggtatc gcgccgccag cgttgctgaa
    14821 catcacgtcg aggcgcccga aggccgcgac cgcgtgcgcg accatggtct gcacctggtc
    14881 ggcgtcggtg acgtcggccg catgggcctt cacgcgtgcg gccggcagtt cctccacggc
    14941 tgcggccagc tgcgcggcgt tgatgtcgac ggccagcacc cgcgcgccct cggcggcgaa
    15001 ggcgcgcaat gaggcgcgcc cgatgccgct ggcggcgccg gtgatgatca cgaccttgtc
    15061 ttgcagtccc agctccatcg cgaattcctc ccgtgtgttg tgctcagtgt ccccagcgtt
    15121 gcagcacgcc gcgccggcgc gccgcgatct gcccggcgcg ctgggccggc gtcacgcgct
    15181 tcgtggtggc tggcaggtga cgctcgagat cggcgagttt cacctgggtg gcctgcggtt
    15241 gcgggtaatg gtcggccagc atccagcgga tcgtcatgta accttccgcg aagcccgagg
    15301 gatcgatcca gttcgccact ccgggatcga cgtgggagac caccaggatc agctcgccgt
    15361 cgtcctccag cgccgcgtag tggcagttgg tgccgcacag gcggttgcgg taatccatcg
    15421 aggaccacca gtagttgccg aactccacgg cccagtaccc ggccctgggc ggcttcacgc
    15481 gcacgatcag cgcctcgtcg gcgggaacgg accagtaggc gatcatcggc tcgccgccgg
    15541 gggtggcgtc gagcgcgttg ctgtcgagct ggcgatagga gaggaaggtg ttgggccgcg
    15601 ccttccacat ctcgatcatg ttcgcccagt actccaccga ctcgccgacc cagcgcgcgg
    15661 cgacgccgag gccctcggcg aggcgtggcg cgtccaccac cggcggcggc gcggcggcgc
    15721 cgaggcgctc gatgcgcgcc tgcatcggcg tttcggtctc ccagtcggcg aagaactggc
    15781 ggaaggtcac gcggaaggtg tccggcgtgg tgcgcagcca gttgcccggc tgcgggtgcg
    15841 ggctgcagat cagttcgaag ctgccgtcgg cctcgacgtg gatgtccccg ccgaacagcg
    15901 tgtgcgcgac cttgccgccc cacggcgtgt tgccggtctc gaccgcggtg accgcgaagt
    15961 agcgcgccgt gccgcggtgg ccgctgatgc ggtaggtgtc ggtgccgttg atcgggccgc
    16021 ccacgtagac cgaatcgggg ttgtcgccgc cctgtttgcg gatcgggtcg aagtagtgga
    16081 tcagctccgg gtgcagcggg tcgttgttct cgaggtggaa ctgcagcgca agcccgatgt
    16141 tcgccgccag cgtgcgaaag ccctcggcgc gcgagagatc gccggtgggt acctgcgggc
    16201 ggaacggcag ccggcccgcg ctcttcagtt gctcgcagaa atcgtcccag gcggcgctga
    16261 gcagcgcgtc ttcgttggtg gtgtcggcgg tcacggctgg ctcctcccgg tgaggcggtt
    16321 gatggtcgtg tcgatcagcc cgagcaggta gggcctgtcg taatagcggg gatcgagcag
    16381 cgcgtcgcag gcgaggcccg cgaccaggtg catcagctcg ttggcggtcg cgaccggatc
    16441 gatgccggcg ggtgcctggc ccagcgcgcg cgcggcctgg aggtcggtga cgaagcgctc
    16501 gcgcgtgcgg tcgaggcgtg cgcgctgggc cttgcgcgcg ccggcattca ccgcggccac
    16561 gctccagacg cacaggcgga tcttccagtc ctggcgcgat tccgcgtcga gcggcagcac
    16621 gcaccgcagc cgctcgcgca gtgcgtccat tccctgcagg ccgcgggtgc ggcgcgtctc
    16681 gcgtgccacg tagcgggcgt tcgcccattc cagcgcggtc tcgatcacct cgtcgcggtt
    16741 ggcgaagtag tgctctacga tgcccttgga gacgccggag cgcgcggcaa tctcgcgcat
    16801 cgtggcctgc tcgaggccga gctcggcgat cgcgagcgcg gcgctgcgcg cgatcgaggc
    16861 gcggcgttcg gcgtggtcga cgatacgggg cacggcggct ttcgggcctt gttgttgttc
    16921 ggcgaggtgc attctccgcg cccgccggat tttttgcaag ccacctggct tgcaacaaca
    16981 tgtaggtcgc attcatgccg acgggtgcgt gtgggtcggc atgaatgccg gcgggtgcct
    17041 gctggtatgc gacgggtccg gctttgccga accctcgtcg ggctgaagcc cgatctgcga
    17101 gcctggccaa ccgtcatcgg atcgtaaccc gtcgcggctt ccctgccgcg ct
//
LOCUS       DAORUQ010000057        17117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3037_length_17117_cov_6.393213, whole genome shotgun sequence.
ACCESSION   DAORUQ010000057 DAORUQ010000000
VERSION     DAORUQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17117
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_17117_cov_6.393213"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLW13_06960"
     CDS             complement(<1..523)
                     /locus_tag="PLW13_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70148.1"
                     /translation="MRTTGFLLRVTCSLLVAFALAGNVTAQKKDDEKAPPPTGIDAAT
                     GKILTEAIEALNKENYAGAKAAVAKLKLDALSPYERSRTEQILATIESSRDNYSGAQK
                     HLQAAIDAGGLNEKEVSDTRYQIAQMFLAQEKWKEGAASLEQWFQTAQNPNSAAYYLL
                     AVAYYQQNDYKRAI"
     gene            complement(526..1122)
                     /locus_tag="PLW13_06965"
     CDS             complement(526..1122)
                     /locus_tag="PLW13_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY70149.1"
                     /translation="MIIRLNIALIFGALLTVAMFWVMWKLISGPIDAASMRTATRIEF
                     TRMRKDTEVASKREQKVERERPPPAPEVPRMAFSSGSVESNVATLTPTVDAGSAMSKM
                     SLSAGSDRDTIPLVRVPPDYPQRALSRGLEGWVQVQFTITGTGSVTDAVVVKSSNKIF
                     EEAALKSIARWRYNPKIEGGVAVDRVGVQTIIRFTLEN"
     gene            complement(1148..1561)
                     /locus_tag="PLW13_06970"
     CDS             complement(1148..1561)
                     /locus_tag="PLW13_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY70150.1"
                     /translation="MQARRHSSEGEDHGIDLAPMLDFTINLLIFFIITTSFIKEPGIT
                     VFRPLAVTAEERPSGNLLVAIRENGDIWIDRRAVELREVRGIMERLHVERPDDSVVII
                     ADRAARAGMVAKVMDEVRLGGIKDVAIAADTDAGG"
     gene            complement(1583..1993)
                     /locus_tag="PLW13_06975"
     CDS             complement(1583..1993)
                     /locus_tag="PLW13_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY70151.1"
                     /translation="MRLKRRVQASHESSHGIDLAPMLDFVINLLIFFIITAVFVKATA
                     VQVNRPTGFEQPNEDDSKSIQIQVLDNGEVWVDNRSIDVRAVRANVERMSAVHPDAGV
                     LILANEMAPSGVVVEVVDQVHLGGIYNITFTTTN"
     gene            complement(1996..2517)
                     /locus_tag="PLW13_06980"
     CDS             complement(1996..2517)
                     /locus_tag="PLW13_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY70152.1"
                     /translation="MLENIPLLNGIVHLLADGGPFVGWIFASGVLLWGLVIERIWYFW
                     RILPAQAAALQAQWMARADRSSWSARQIRQRMISRLNESMTVGFPVMQVLVPMSPLLG
                     LIGTVTGMLEVFDSMALRGNADARSMASGVSEAMICTMTGLAVSITGLYPVHYFRTRA
                     ERETELLADRLPL"
     gene            complement(2510..3712)
                     /locus_tag="PLW13_06985"
     CDS             complement(2510..3712)
                     /locus_tag="PLW13_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY70153.1"
                     /translation="MLAEAQAKRDALKARSDALSGQFSANDLKINELGKQLQQKAVSL
                     GLAEVFGLSRQVAGDSATVLEQSLISTQYPPAAGEPDRAEALRAFAASKGVPVTADLE
                     RVWFELQREMVGSGQVARYKANVVQPGGESASADVVRVGPFTAVSGGKVLHYLPALQS
                     LNVYPRQPPQDILNHVRNLESATEGYVQAVVDSSRGVLMALYVERPTWGQRIEHGESI
                     NYVILAVGLIAFLAFLWQLVYLVLVRLRVSAQLKNLDKPVKDNPLGRVLLAFKGDAKT
                     IEQEADVAELRISEAVFREVPKLERFQALLRLAVAAGPLLGLIGTVLGMIMTFQSITE
                     SGSSDPKLMADGIGAAMIATVLGLGIAIPLLFGNALLTALSRGVTQVIEEQSAGMLAE
                     SIERQARA"
     gene            complement(3901..4689)
                     /locus_tag="PLW13_06990"
     CDS             complement(3901..4689)
                     /locus_tag="PLW13_06990"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HQY70154.1"
                     /translation="MTPSNPRITRALALLISAGAAALVAAPVGAQTLDSALGAQGEAD
                     RAAAASQKRINEIRDRTQDASSRYASAIADAESLEKYNSQLDKQVAAQREEMASIEKQ
                     LIDIETTNREVQPLMQRMVDTLERFVALDVPFLQDERKARVESLQEMMLRADVTISEK
                     YRRIMEAYQIELENGRTLDAYEGLLGSGGDARTVSFVRLGRVALMYQSLDGAETGYWD
                     VNSKSWVVDNSYADAVAEARRVAEKDGAPDLLTVPVPAPQEVRQ"
     gene            5063..5467
                     /locus_tag="PLW13_06995"
     CDS             5063..5467
                     /locus_tag="PLW13_06995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQY70155.1"
                     /translation="MNPGLEALSHNWAVAVFVAVILLVCVLMLAVSALTGGRTRGRAK
                     TDPFESGIVSVGGAHLRLPARFYLVAMFFVIFDVEALFLYAWAVSLRESGWIGFIEAA
                     TFIFILTAGLVYLWRIGALRWAPAPRRRPREQ"
     gene            5475..6131
                     /locus_tag="PLW13_07000"
     CDS             5475..6131
                     /locus_tag="PLW13_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQY70156.1"
                     /translation="MDYRLTPIATDAPVPVQAAADPLSQQVHRSVFMGKLEDVLHKAV
                     GWGRKNSLWPYNFGLSCCYVEMATAFTAPHDVARFGAEVIRASPRQADFMVIAGTCFI
                     KMAPVVQRLYEQMLEPRWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRP
                     EAFLQGLMLLQDSIATERRPLSWVVGDQGVHKLEVPSQRELRREARMSATTLRTPDQV
                     "
     gene            6172..7953
                     /gene="nuoC"
                     /locus_tag="PLW13_07005"
     CDS             6172..7953
                     /gene="nuoC"
                     /locus_tag="PLW13_07005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HQY70157.1"
                     /translation="MKKHRPSPQENLPGSEHGVIAELYRRFGADAIAYQPTVDGLPTA
                     WVPRAQLPEVLRFLRDAPRPYAMLYDLHGVDERLRTHRESLPAADFTVFYHLLSLERN
                     SDVRIKVALSSDDLTLPTVTGIWPNANWYEREVWDLYGIGFSGHPNLQRIMMPRTWTG
                     HPLRKDYPARATEFDPFSLDAAKQDLEQESLRFVPEEWGMSRSTAHSDFMFLNLGPNH
                     PSAHGAFRIVLQLYGEEIHDCVPDIGYHHRGAEKMAERQSWHSFIPYTDRIDYLGGVM
                     NNLPYVMAVEQLAGIRVPDRVKVIRVMLAEFFRITSHLLFLGTYIQDVGGMSPIFFIF
                     TDRMKAYDVIEAITGGRMHPAWYRIGGVAHDLPNGWDRLVREFCDWMPGRLDEYEKAA
                     MRNGILRSRTIGVAQYNTREAIEWGVTGASLRATGLDYDLRKARPYSGYENFDFEVPL
                     AHNGDAYDRAMVRLEEMRQSVRIIRQCLDNMPAGPYKADHPLTTPPPKERTLQHIETL
                     INHFVGVSWGPVMPAGEACQMIEATKGINSYYLTSDAGTMSYRTRIRTPSFAHLQQIP
                     SVIRGSLIADLIAYLASIDFVMADVDR"
     gene            7964..8455
                     /gene="nuoE"
                     /locus_tag="PLW13_07010"
     CDS             7964..8455
                     /gene="nuoE"
                     /locus_tag="PLW13_07010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQY70158.1"
                     /translation="MIASSAPGFRLSEAELAEIHHEMSHYEDPRAASIEALKAVQKHR
                     GWVPDAAIDAIAAVIGIPASDVEGVATFYNMIFRRPVGRHVIKVCDSISCFLTGYDAL
                     RDALMAQTGLGYGETGADGRYTLLPVCCLGNCDKGPTLMIDDDTHGPVAPADVPALLE
                     RYT"
     gene            8452..9759
                     /gene="nuoF"
                     /locus_tag="PLW13_07015"
     CDS             8452..9759
                     /gene="nuoF"
                     /locus_tag="PLW13_07015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQY70159.1"
                     /translation="MSQRPRDTHPLTWRLDHGAPVLGIDAHEALDGYAALRKALGMEP
                     AAVTQAVKDSNLRGRGGAGFPTGVKWSLVPMGPDAGTKYLLCNADEMEPGTFKDRMLL
                     EQLPHQLIESMVIAAWAIQATRGYIFLRGEYVLAAERLQRALAEARDSGYLGENIRGT
                     GWSFDLFLHTGAGRYICGEETALINSLEGRRANPRAKPPFPQIAGAWGRPTIVNNVET
                     FYNLPEILLQGADWYKGLSLGRSTDGGTKIYGASGRVKRPGLWELPMGTTGREILEQH
                     AGGMQDGLQLKAWLPGGASTDFLLPEHLDLAMDYDTIAKARSRLGTGLLAVVDHTQDI
                     VPAVRNLLEFFARESCGWCTPCRDGLPWSVKLLRAIERGEGQPGDIERLERLTVQLGP
                     GKTYCALAPGAVEPLQSALRFFRAEFERGIKGGAQGAARKEHA"
     gene            9759..12461
                     /gene="nuoG"
                     /locus_tag="PLW13_07020"
     CDS             9759..12461
                     /gene="nuoG"
                     /locus_tag="PLW13_07020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HQY70160.1"
                     /translation="MATIHIDGTSYEVEGSDNLLHACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYANADDTRGRLVMSCMTPSSDNTYISIGDEEAREFRKGVIEFLMTNHPHD
                     CPVCEEGGHCHLQDMTVMSGHNSRDYRFTKRTHRNQDLGPFVNHEMNRCIACYRCVRY
                     YKDYAGGSDLGVFGAHDNVYFGRVADGRLESEFSGNLTEVCPTGVFTDRTHSERYNRK
                     WDMQFAPSVCQQCAVGCNTSPGERYGELRRIENRFNGEVNHYFLCDRGRFGYGYVNRA
                     DRPRQMLARAGSEFEAATLQGGLERLAEAMRSASRLVGIGSPRASLEANFALRHLVGA
                     DNYSTGMDGTEAALLAQALGILRTGAARTPSLREIETFDAILVLGEDLTQTAPRIALA
                     LRQAVRGQATELAASKKISAWQAEAIATIGQKERNPLFIASVDATRLDDAAHASWRAT
                     PARIAALGAAVARALDPAAPASGSDAEIDAFAEQVAAALAGAKKPLVVSGTGLRSGAI
                     LDACANIAVALKARGQEPALTLALPEANSLGVAMLGGNAVEDALASVDANSVLVVLEN
                     DLYRRADAALVDAALARARCVVVIDHQLSATGAKAHLLLPAASFAEGDGTLVSNEGRA
                     QRYFQVYDPAYYNIATQVRESWRWLAGIQSAENRVPVEWETLDQITAACARENPALAG
                     IVAAAPGADTRVHGRRVARQPNRYSGRTAMRANISMHEPRAPQDPDSALAFSMEGYGG
                     PDEPGPLVPFAWSAGWNSPQAWNKFQDEVGGHLRQGDPGIRLIEPAASAGYVPVATAA
                     ASDLAVAPLHHIFGSEELSARAPVTAQNVPAPYVALHPREARRRGLGAGVTVRVSVGA
                     VQFEAPLALRESLSEGVIGIPAGLPGMGFAFVAATASVSTGGQS"
     gene            12458..13438
                     /gene="nuoH"
                     /locus_tag="PLW13_07025"
     CDS             12458..13438
                     /gene="nuoH"
                     /locus_tag="PLW13_07025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQY70161.1"
                     /translation="MSFLSPEVAAVLWAVVKAVIILLAAVIFAAYLSFIERRVLALWQ
                     DRYGPNRVGPFGIFQLPADVVKMLFKEDWIPPFADKLTFLIAPIIAMSALFISYIIVP
                     VTPTWYVADFDIGILFFLAMAGLAVYAVLFAGWSSNNKYSLLGGLRSAAQTVSYEVFM
                     GLSLMGVVAQSGSFNMREIVEAQRDLWFIVPQFLGFCTFALAGIAVTHRSPFDLPEAE
                     QELAAGYHTEYAGMKWAMFFVGEYIGIVVVSSLLTTLFLGGWLGPGPDWLAPLWFVAK
                     TLVFVTLFILVRAALPRPRYDHLMTAGWMVCLPITLLNLLVTGAVLLAKG"
     gene            13443..13988
                     /gene="nuoI"
                     /locus_tag="PLW13_07030"
     CDS             13443..13988
                     /gene="nuoI"
                     /locus_tag="PLW13_07030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQY70162.1"
                     /translation="MNLLLKILAGIGTNLRSMWMIFSRATRARATIQYPEQAVYLPPR
                     YRGRIVLTRDPDGEERCVACNLCAVACPVGCIALQKAEKDDGRWYPEFFRINFSRCIF
                     CGMCEEACPTTAIQLTPDFELAEYRREDLVYEKEDLLIAGSGKNPEYNFYRVAGMAIA
                     GKPKGAAQGEAQPIDVRTLLP"
     gene            14002..14493
                     /gene="nuoJ"
                     /locus_tag="PLW13_07035"
     CDS             14002..14493
                     /gene="nuoJ"
                     /locus_tag="PLW13_07035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQY70163.1"
                     /translation="MELTFYTCALVAILATLRVVTNANPVHALLYLVVSLLAVAGVFF
                     ALGAPFAGALEVIVYAGAIMVLFVFIVMMLNLGPETTAQERQWLKPATWIGPMLLSAL
                     LLGALVFTLVGADAPTGSATVGPKAVGIALFGPYVLVVELASLLLLGALVAAYHLGRD
                     DHH"
     gene            14526..14843
                     /gene="nuoK"
                     /locus_tag="PLW13_07040"
     CDS             14526..14843
                     /gene="nuoK"
                     /locus_tag="PLW13_07040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQY70164.1"
                     /translation="MIEAASVPLEHALALAAVLFCTGLAGLMVRRNFLFMLMSLEVMT
                     NAAALAFVAAGSRWAQPDGQIMFIMVLALAAAEASIGLALLLQLYRRFHTLDIDVASE
                     MRG"
     gene            14840..16675
                     /gene="nuoL"
                     /locus_tag="PLW13_07045"
     CDS             14840..16675
                     /gene="nuoL"
                     /locus_tag="PLW13_07045"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQY70165.1"
                     /translation="MSFLYLTFLAPLAGFLLLAFSRGRLGENAAAIIGAGSVGVSALV
                     TALAAAQFSVPVTQVLWTWMHIGDFAPRFALYLDGLSLTMLGVVTGVGFLIHVFASWY
                     MRGEEGYSRFFAYMNLFVASMVMLVLADNLAFLYLGWEGVGICSYLLIGFYYRTVANG
                     NAAIKAFLVTRVGDVFMAIGLFILLSQLGTLDIQELLARAPERFASGDPLIVAATLML
                     LGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFELAPHVL
                     ELVGAVGAVTLLIAGLCALVQTDIKRVLAYSTMSQIGYMFLALGAGAYSAAIFHLMTH
                     AFFKALLFLAAGAVIVACHHEQNIFRMGGLRKRLPLEYASFLVGGGALCALPFVTAGF
                     FSKDEILWAEYTTGHQFLFLAGVAGAFLTSLYTFRLIFTVFHGEQHTEAHAGRGLAYS
                     VPLITLLVLSTGLGAMIHPPLAEVLPVLKGQVAEGERHFVEILAAGVGIAGIALAAVL
                     FLGKRTLATGLANSAAGRFVGNWWLNAFGFDALYNLLFVRPFLFIARPGRRDALDGTI
                     GLLPVGVRALNRLVVLPQTGRLRWYVASIALGAVLVVGFVVINLV"
     gene            16691..>17117
                     /locus_tag="PLW13_07050"
     CDS             16691..>17117
                     /locus_tag="PLW13_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQY70166.1"
                     /translation="MLLAWLILIPIVGGLLCWQTDRFGNLPRWIALGSMTTVFALSLE
                     LWATGNYSLAHGIHDVPQWQMEFVMPWIPSLGISIHLGVDGLSLALVVLTGFLGILSV
                     LCSWREIQRRTGFFHLNLMWIIGGVVGVFLALDLFLFFLF"
BASE COUNT         2663 a         5949 c         5609 g         2896 t
ORIGIN      
        1 cgatcgcgcg cttgtaatcg ttctgctggt agtaagccac cgcgagcaga tagtaggccg
       61 cgctgttggg gttctgcgcc gtctggaacc attgttccag cgaggccgcg ccttccttcc
      121 acttctcctg cgccaggaac atctgcgcga tctggtagcg cgtgtccgac acttccttct
      181 cgttcaggcc gccggcgtcg atggccgcct gcaggtgctt ctgcgcaccc gaatagttgt
      241 cgcgcgagct ctcgatcgtg gccaggatct gctcggtgcg gctgcgctcg tagggactca
      301 acgcgtcgag cttgagcttg gccacggccg ccttggcacc ggcgtagttt tccttgttca
      361 gcgcttcgat cgcctcggtc aggatcttgc cggttgcggc atcgataccc gtgggcggag
      421 gcgccttctc atcatccttt ttctgcgccg tgacgttgcc ggccagcgca aaggctacca
      481 gcaacgaaca ggtgacgcgc agcaggaatc cggtcgtccg cattactagt tctccagggt
      541 gaagcggatg atggtctgga cgccgacgcg atccaccgcg acgccaccct cgatcttggg
      601 gttgtaacgc cagcgcgcga tcgacttgag agccgcttcc tcgaagatct tgttcgagga
      661 cttcactacc acggcatccg tgaccgatcc ggtgccggta atcgtgaact gcacctgcac
      721 ccagccttcc aggccgcgac tgagcgcgcg ctgaggatag tccggcggca cgcgcaccaa
      781 cgggatggtg tcgcggtcgg aaccggcgct cagcgacatc ttcgacatcg ccgagcccgc
      841 atcgacggtc ggtgtcagcg tcgcgacgtt gctctccacg ctgccggaac tgaacgccat
      901 gcgcggcact tcaggcgcgg gcggcggacg ctcgcgctcg accttctgct cgcgcttgct
      961 cgccacttcc gtgtccttgc gcatgcgcgt gaactcgatg cgcgtcgctg tacgcatgct
     1021 ggccgcatcg atgggcccgc tgatcagctt ccacatgacc cagaacatgg ccactgtaag
     1081 cagggcaccg aatatcagcg caatattcaa cctgatgatc aagacgactt actccccacg
     1141 ttgccgttca gccgcccgca tccgtgtcgg ctgcgatggc tacatccttg ataccgccca
     1201 ggcggacctc gtccatgacc ttcgccacca tgcccgcgcg cgccgcgcgg tcggcgatga
     1261 tcacgacgct gtcgtccgga cgctcgacgt gcagacgctc catgatgccc cggacttccc
     1321 tcaattccac cgcgcggcgg tcgatccaga tgtcgccgtt ctcccgtatc gccacgagca
     1381 ggttgcccga cggacgttct tcggcggtga ccgccagggg acggaatacc gtgattcccg
     1441 gctccttgat gaaggatgtc gtgatgatga agaagatcaa caggttgatc gtgaagtcga
     1501 gcatcggcgc caggtcgatg ccatgatcct cgccttcgga agaatgtcgt cgtgcctgca
     1561 cggtcgtgtc ctcgtagttc gcctagttcg tggtggtgaa ggtgatgttg tagatgccac
     1621 cgaggtgcac ctggtcaacc acttcgacca ccacgcccga gggcgccatt tcattcgcca
     1681 ggatcagcac gccggcgtcc ggatggaccg cgctcatgcg ctcgacgttg gcgcgcacgg
     1741 cacgcacgtc gatcgagcgg ttgtccaccc acacctcgcc gttgtccagg acctggatct
     1801 ggatgctctt cgaatcgtcc tcgttcggct gctcgaaacc ggtcgggcgg ttcacctgca
     1861 ccgcggtcgc cttcacgaac accgcggtga tgatgaagaa gatcagcagg ttgatgacga
     1921 agtccagcat gggagcgagg tcgatcccgt gggacgattc atgtgaggct tgtacgcgac
     1981 gcttgagacg catgattaca gtggcaacct gtctgctaga agttcggttt cccgctcggc
     2041 acgggtgcgg aagtaatgca ccgggtacag tcccgtgatg ctcaccgcaa gtcccgtcat
     2101 cgtgcagatc atcgcttcgg aaacaccgct cgccatgctg cgggcgtcgg cgttaccgcg
     2161 cagtgccatg gagtcgaata cctcgagcat tccggtcacg gtcccgatga ggccgagcaa
     2221 cggcgacatc ggaaccagga cctgcatgac cgggaaaccg accgtcatgg actcgttcag
     2281 ccgcgagatc attctctggc gtatctggcg cgcgctccac gagctgcggt cggcgcgtgc
     2341 catccactgg gcttgcaacg ccgcagcctg ggccggcagt attcgccaga agtaccagat
     2401 acgctcgatc acgagccccc agagcaacac gcccgaggcg aagatccatc cgacgaacgg
     2461 gccgccgtcc gccagcagat ggacgattcc gttcagcaga gggatgttct caagcacggg
     2521 cctgcctctc gatactctcg gccagcatgc cggccgactg ttcctcgatg acctgggtca
     2581 cgccgcgcga cagcgcggtc agcagcgcgt tgccgaacag cagcggaata gctatgccga
     2641 ggcccagcac cgttgcaatc atcgccgcgc cgataccatc ggccatcagc ttgggatcgc
     2701 tggagcccga ttccgtgatg ctctggaacg tcatgatcat accgagcacg gtgccgatca
     2761 ggccgagcag gggaccggcg gcgaccgcga ggcgcagcag tgcctggaag cgctcgagct
     2821 tcggcacctc gcggaacacg gcctcggaga tgcgcagctc ggcgacgtcg gcttcctgct
     2881 cgatggtttt cgcatcgccc ttgaaggcca gcagaacacg acccagcgga ttgtccttga
     2941 ccggcttgtc caggttcttg agctgtgccg acacgcgcag acgcacgagc accaggtaga
     3001 cgagttgcca gaggaacgcg aggaaggcaa tcaagccgac ggcgaggatg acgtagttga
     3061 tgctctcgcc gtgctcgatg cgctggcccc acgtgggacg ctcgacgtac agcgccatca
     3121 gcacgccgcg ggaggagtcg acgaccgcct gcacgtagcc ttcggtggcc gactcgagat
     3181 tcctcacgtg attcaggata tcctgcggcg gctggcgcgg gtacacgttg agcgactgca
     3241 gggccggcag gtaatggagc accttgccgc cggataccgc ggtgaacggc cccacgcgca
     3301 cgacgtcggc cgacgccgat tcgccgccgg gctggaccac gttcgccttg tagcgggcga
     3361 cctggccgct gccgaccatc tcgcgctgca gctcgaacca cacgcgctcg aggtcggcgg
     3421 tgaccggaac gcccttcgac gccgcaaagg cacgcagcgc ctctgcacgg tcaggctcac
     3481 cggccgccgg cgggtactgg gtcgagatca gcgactgctc caggacggtc gcgctgtcgc
     3541 cggcaacctg ccgcgacagg ccgaacacct cggcgaggcc caggctgacg gccttttgct
     3601 gcagttgttt gccgagctcg ttgatcttga gatcgttggc ggagaactgc cccgagagcg
     3661 catccgaccg ggccttgagc gcatcgcgct tggcctgggc ctcggccagc atcttctcct
     3721 ggtcggcagc ggaggcggcg ttgtaggccg cgctgcgctg ctcgaaggcc tgcgagcttg
     3781 ccgaccgacc cgccgcgact tcctgcaaca gcgcatcgag gctgtcagcc gcgaacgccg
     3841 gagtgcctgc gtgcagcagc gttgcgactg ccgcgtactt gaagagcctg tggaacttga
     3901 tcactgccgt acctcctgcg gggcgggaac cggaaccgtc agcaggtcgg gcgcaccatc
     3961 tttctcggcg acgcgacggg cttccgccac ggcgtccgca tagctgttgt caaccaccca
     4021 cgacttcgaa ttcacatccc agtagcccgt ttccgcgccg tccagcgact ggtacatcag
     4081 cgcgacgcga cccaggcgga cgaacgacac cgtgcgcgca tccccgcccg agccgagcag
     4141 cccttcgtag gcgtcgagcg tgcgaccgtt ctccagttcg atctggtagg cctccatgat
     4201 gcggcgatat ttctcggaga tcgtgacatc ggcgcggagc atcatttcct gcagcgactc
     4261 gacccgcgct ttgcgctcgt cctgcaggaa gggcacgtcg agcgccacga agcgctccag
     4321 cgtgtcgacc atgcgctgca tcaggggctg cacttcgcgg ttggtggtct cgatgtcgat
     4381 gagctgcttc tcgatggaag ccatctcctc gcgctgcgcg gcaacctgct tgtcgagctg
     4441 ggagttgtac ttctcgaggc tctccgcatc ggcaatggcc gaggcgtagc ggctcgaggc
     4501 gtcctgcgtg cggtcgcgga tctcgttgat gcgcttctgc gacgccgccg ccgcgcggtc
     4561 agcctcgccc tgcgcgccca gcgccgaatc gagcgtctgc gcgccaactg gggcggcaac
     4621 cagggcggcc gcaccggcac tgatcaagag ggccagggcc ctcgtgatcc tgggattgga
     4681 aggggtcatg acgggtagcg cctcctgatc aaagagttcg actcagcggg aaagggtgac
     4741 attggacaaa caaccatcga atcgcctgtt gtactttttg gcggctgcgt ccggcatggt
     4801 gcgggacggc ctgaatcgct cgtgcgggcg aactctaata gagcccattt aatgttgcaa
     4861 gctgttccag ccagggaaag caaatctttt ttgaagagtt ttcaacagcg ctccggacag
     4921 gcttcgggaa tatgccgcga gcccccgatc gcggcatgtt caatcacgat cgatcaaata
     4981 taatggcgcc cgattccagg ggtcacggag cctccgcttc cagcctgctc cgcggcatat
     5041 ctccccgatg ccggcaccgc acatgaatcc aggtctcgaa gcgctgagtc ataactgggc
     5101 cgtggccgtc ttcgtggccg tgatcctcct cgtctgtgtc ctgatgctcg ccgtgtccgc
     5161 cctgacgggc gggcgcacac gcgggcgcgc caagaccgat cccttcgaat ccgggatcgt
     5221 gtcggtgggc ggcgcccacc tgcggctacc cgccaggttc tatctcgtgg cgatgttctt
     5281 cgtgatcttc gacgtcgagg cactctttct gtatgcgtgg gccgtctcgc tgcgcgagag
     5341 cggctggatc ggcttcatcg aggccgcgac cttcattttc atactgacgg ccggcctggt
     5401 ctacctgtgg cggatcggcg cgttgcgctg ggcgcccgcc ccgcgtcgcc ggccgcgcga
     5461 gcagtgaggt accgatggac taccgcctga ccccgatcgc caccgacgcg cccgtcccgg
     5521 tccaggcagc ggccgacccg ctgtcgcagc aggtccatcg cagcgtgttc atgggcaagc
     5581 tcgaggacgt gctgcacaag gccgtgggct ggggacgcaa gaactcgctg tggccctaca
     5641 acttcggcct gtcctgctgc tacgtcgaga tggcgaccgc cttcaccgcg ccgcacgacg
     5701 tcgcgcgctt cggcgccgag gtgatccgcg cctcgccgcg ccaggcggac ttcatggtga
     5761 tcgcgggtac ctgcttcatc aagatggccc cggtggtcca gcgcctctac gagcagatgc
     5821 tggagccgcg ctgggtgatc tcgatgggtt cctgtgccaa ctcgggcggc atgtacgaca
     5881 tctattccgt ggtgcagggc gtggacaagt tcctgcccgt cgacgtgtac atcccgggct
     5941 gcccgccgcg acccgaggcc ttcctgcagg ggctgatgct gctgcaggat tccatcgcca
     6001 ccgaacgccg cccgctgtcg tgggtggtgg gcgaccaggg cgtccacaag ctcgaggtgc
     6061 cctcgcagcg cgaactgcgg cgcgaggcgc gcatgagcgc cacgacgctg cgaacccccg
     6121 accaggtatg aaagggatcc acgtgcgcac gctgagcaga ccggttcagg gatgaagaaa
     6181 cacagaccca gcccgcagga gaacctgccc ggcagcgagc acggcgtcat tgccgagctc
     6241 taccgccgct tcggcgccga cgcgatcgcc taccagccga ccgtggacgg cctgccgacg
     6301 gcatgggtgc cgcgcgcgca actgcccgag gtgctgcgct tcctgcgcga cgccccgcgc
     6361 ccctacgcca tgctctacga cctgcatggc gtggacgagc ggctgcgcac gcatcgcgag
     6421 agccttccgg ccgcggattt cacggtcttc tatcacctgc tgtcgctgga gcgcaacagc
     6481 gacgtgcgca tcaaggtggc gctgtcgagc gatgacctga cgctgccgac ggtcaccggg
     6541 atctggccga acgccaactg gtacgagcgc gaggtgtggg acctctacgg catcggcttc
     6601 agcggacatc ccaacctgca gcgcatcatg atgccgcgca cctggaccgg ccacccgctg
     6661 cgcaaggact acccggcgcg cgccaccgag ttcgacccct tctcgctcga cgcggccaag
     6721 caggacctgg agcaggagtc gctgcgcttc gtgcccgagg aatggggcat gagccgcagc
     6781 accgcgcact cggacttcat gttcctgaac ctgggaccca accacccctc ggcgcacggg
     6841 gccttccgca tcgtgctgca actctacggc gaggaaatcc acgactgcgt ccccgacatc
     6901 ggttaccacc accgcggtgc cgagaagatg gccgaacgcc agtcctggca cagcttcatc
     6961 ccgtacaccg accgcatcga ctacctcggc ggggtgatga acaacctgcc ctacgtgatg
     7021 gcggtcgagc agctcgccgg catccgggtg ccggatcgcg tgaaggtgat ccgcgtgatg
     7081 ctggcggagt tcttccgcat caccagccac ctgctgttcc tgggcaccta catccaggac
     7141 gtcggcggca tgtcgccgat cttcttcatc ttcaccgacc gcatgaaggc ctacgacgtg
     7201 atcgaggcca tcaccggcgg gcgcatgcac ccggcctggt accgcatcgg cggcgtggcg
     7261 cacgacctgc ccaacggctg ggatcgcctg gtgcgcgagt tctgcgactg gatgcccggg
     7321 cgcctcgacg agtacgagaa ggccgcgatg cgcaacggca tcctgcgctc gcgcaccatc
     7381 ggcgtggcgc agtacaacac gcgcgaggcc atcgagtggg gggtgaccgg cgcgagcctg
     7441 cgcgccaccg gtctcgacta cgacctgcgc aaggcgcggc cctattccgg ctacgagaac
     7501 ttcgacttcg aggtgccgct ggcgcacaac ggcgacgcct acgaccgcgc gatggtgcgc
     7561 ctcgaggaga tgcgccagag tgtccgtatc atccgccagt gcctggacaa catgccggcg
     7621 ggcccgtaca aggccgacca cccgctgacg acgccgccgc cgaaggagcg cacgctgcag
     7681 cacatcgaga cgctgatcaa ccatttcgtg ggcgtgtcgt ggggaccggt gatgccggcc
     7741 ggggaggcct gccagatgat cgaggccacc aagggcatca acagctacta cctgacctcg
     7801 gacgccggca ccatgagcta ccgcacgcgc atccgcaccc cgagcttcgc gcacctgcag
     7861 cagatcccct cggtgatccg cggcagcctg atcgcggatc tcatcgcgta cctcgcaagc
     7921 attgatttcg tgatggcaga tgtcgacaga taacgcaccc cagatgatcg cgagcagcgc
     7981 ccccggtttc cgcctgtcgg aagccgagct ggcggaaatc catcacgaga tgagccacta
     8041 cgaggacccg cgcgcggcct cgatcgaggc gctgaaggcg gtgcagaagc accgcggctg
     8101 ggtgcccgac gcggccatcg acgccatcgc cgcggtgatc ggcattccgg cctccgacgt
     8161 cgagggcgtg gcgacgttct acaacatgat tttccgccgc ccggtcggac ggcacgtgat
     8221 caaggtctgc gacagcatct cctgcttcct caccggctac gacgcgctca gggacgcgct
     8281 gatggcgcag accggtctcg gctacggcga gaccggcgcc gacggccgct acacgctgct
     8341 gccggtgtgc tgcctcggca actgcgacaa gggcccgacg ctgatgatcg acgacgacac
     8401 gcacggcccg gtggcgcccg ccgacgtgcc ggcgctgctg gagcgctaca catgagccag
     8461 cgcccgcgcg acacccaccc gctgacctgg cgcctcgatc acggcgcgcc ggtgctcggc
     8521 atcgacgccc acgaagcgct ggacggctac gccgcgctgc gcaaggcact cggcatggaa
     8581 cccgccgcgg tcacccaggc ggtgaaggac tcgaacctgc gcgggcgcgg cggcgcgggc
     8641 ttcccgaccg gcgtgaagtg gagcctggtg ccgatggggc cggacgccgg caccaagtac
     8701 ctgctgtgca acgccgacga aatggagccc ggcaccttca aggaccgcat gctgctcgag
     8761 cagctgccgc accagctcat cgagtcgatg gtcatcgcgg cctgggcgat ccaggccacg
     8821 cgcggctaca tcttcctgcg cggcgaatac gtgctggccg ccgaacgcct gcagcgcgcg
     8881 ctggccgagg cgcgcgacag cggctacctg ggcgagaaca tccgcggcac cggctggagt
     8941 ttcgatctct tcctgcatac cggggccggg cgctacatct gcggcgagga gacggcgctg
     9001 atcaactcgc tcgagggccg gcgggccaac ccgcgcgcca agccgccgtt cccgcagatc
     9061 gccggcgcct ggggccggcc gacgatcgtc aacaacgtcg agaccttcta caacctgccc
     9121 gagatcctgc tgcagggcgc cgactggtac aagggcctgt cgctggggcg cagcaccgac
     9181 ggcggcacca agatctacgg cgcctcgggg cgcgtgaagc gtcccgggct gtgggaactg
     9241 ccgatgggta ccacggggcg cgagatcctc gagcagcacg ccggcggcat gcaggacggc
     9301 ctgcagctga aggcctggct gccgggcggc gcttccaccg acttcctgct ccccgagcac
     9361 ctcgacctcg cgatggacta cgacacgatc gccaaggccc gcagccgcct cggcaccggc
     9421 ctgctcgccg tggtcgacca cacccaggac atcgtccccg cggtgcgcaa cctgctggag
     9481 ttcttcgcgc gcgaatcctg cggctggtgc acgccgtgcc gcgacgggct gccatggtcg
     9541 gtgaagctgc tgcgcgcgat cgagcgcggc gaggggcagc cgggcgacat cgaacggctc
     9601 gagcgcctca ccgtgcagct ggggccgggc aagacctact gcgcactcgc gcccggggcc
     9661 gtggagccgc tgcagagcgc gctgcgcttc ttccgcgccg agttcgagcg cggcatcaag
     9721 ggcggcgcgc agggcgccgc gcgcaaggaa cacgcgtaat ggcaacgatc catatcgacg
     9781 gcaccagcta cgaggtcgag ggcagcgaca acctgctgca cgcctgcctc tcgctcggcc
     9841 tggacatacc ctatttctgc tggcacccgg cgctgggctc cgtgggcgcc tgccgccagt
     9901 gcgcggtgaa gcagtacgcc aacgccgacg acacgcgcgg gcggctggtc atgtcgtgca
     9961 tgacaccctc gagcgacaac acgtatatct cgatcggcga cgaggaagcg cgcgagttcc
    10021 gcaagggcgt gatcgagttc ctgatgacca accacccgca cgactgcccg gtgtgcgagg
    10081 aaggcggcca ctgccacctg caggacatga cggtgatgag cgggcacaac agccgcgact
    10141 accgcttcac caagcgcacg caccgtaacc aggacctcgg cccgttcgtc aaccacgaga
    10201 tgaaccgctg catcgcctgc taccgctgcg tgcgctacta caaggactat gccgggggca
    10261 gcgacctcgg cgtgttcggc gcccacgaca acgtctactt cgggcgcgtc gccgacggcc
    10321 ggctcgagag cgagttctcg ggcaacctca ccgaggtctg ccccaccggc gtcttcaccg
    10381 accgcaccca ttccgagcgc tacaaccgca agtgggacat gcagttcgcc cccagcgtct
    10441 gccagcagtg cgcggtgggc tgcaacacca gcccgggcga gcgctacggc gagctgcgcc
    10501 gcatcgagaa ccgcttcaac ggcgaggtga accactattt cctctgcgac cgcggccgct
    10561 tcggctacgg ctacgtgaac cgcgccgacc gcccgcgcca gatgctggcg cgcgcgggct
    10621 ccgagttcga ggccgccacg ctgcagggcg ggctggagcg cctggccgag gcgatgcgct
    10681 cggcctcgcg cctggtcggc atcggctcgc cgcgcgcgag cctggaggcc aacttcgcgc
    10741 tgcgccacct ggtcggcgcg gacaactatt cgaccggcat ggacggcacg gaggccgcgc
    10801 tgctcgcgca agcgctcggg atcctgcgca ccggtgcggc gcgcacgccg tcgctgcgcg
    10861 agatcgagac gttcgacgcg atcctggtgc tcggcgagga cctgacccag accgcgccgc
    10921 gcatcgcgct ggcgctgcgc caggccgtgc gcggccaggc caccgagctc gcggcctcga
    10981 agaagatatc cgcctggcag gccgaggcga tcgccaccat cgggcagaag gaacgcaacc
    11041 cgctgttcat cgccagcgtc gatgccacgc gcctcgacga cgccgcgcac gccagctggc
    11101 gcgccacgcc ggcacgcatc gccgccctcg gcgcggcggt cgcccgcgcg ctcgatcccg
    11161 cggcccctgc cagcggcagc gatgcggaaa tcgatgcctt cgccgagcag gtcgccgcgg
    11221 cgctcgccgg cgcgaagaag ccgctcgtcg tctccggcac ggggctgcgc tcgggggcga
    11281 tcctcgacgc ctgcgccaac atcgccgtgg cgctgaaggc gcgcggacag gagcccgcgc
    11341 tgacgctggc gctgcccgag gccaacagtc tcggggtcgc gatgctcggc ggcaacgccg
    11401 tcgaggacgc gctggccagc gtcgatgcca actcggtgct ggtggtgctg gagaacgatc
    11461 tctaccgccg cgccgatgcc gcgctggtcg atgccgcgct ggcgcgcgcg cgttgcgtgg
    11521 tcgtgatcga ccaccagctg agcgccaccg gcgcgaaggc gcacctgctg ctgccggcgg
    11581 cgagcttcgc cgagggcgac ggcacgctgg tcagcaacga gggccgcgcg cagcgctatt
    11641 tccaggtcta cgatcccgcc tactacaaca tcgccaccca ggtgcgcgag agctggcgct
    11701 ggctggccgg catccagagc gcggagaacc gcgtgccggt ggaatgggag acgctggacc
    11761 agatcaccgc ggcctgcgcg cgcgagaacc ccgcgctcgc cggcatcgtg gctgccgcgc
    11821 cgggggccga cacccgcgtg cacgggcgcc gcgtggcacg ccagccgaac cgctattcgg
    11881 gccgcaccgc gatgcgcgcg aacatctcga tgcacgaacc gcgtgccccg caggatcccg
    11941 acagcgcgct ggccttctcg atggagggct acggcggccc cgacgagccg ggtccgctgg
    12001 tgcccttcgc gtggagcgcg ggctggaact cgccgcaggc ctggaacaag ttccaggacg
    12061 aggtcggcgg gcacctgcgc cagggcgatc ccggcatccg cctgatcgag ccggcggcga
    12121 gcgcgggcta cgtgcccgtc gccaccgccg ccgcgagcga tctggcggtc gcgccgctgc
    12181 accacatctt cggcagcgag gaactgtcgg cgcgcgcgcc ggtgaccgcg cagaacgtgc
    12241 cggcgcccta cgtggcgctg catccgcgcg aggcccggcg ccgcggcctc ggcgccggcg
    12301 tgaccgtgcg cgtcagcgtc ggcgcggtgc agttcgaggc gccgctggcg ctgcgcgaat
    12361 cgctcagcga aggcgtcatc ggcatccccg ccgggctacc gggcatgggc ttcgccttcg
    12421 tcgccgccac tgccagcgtc agcaccggag ggcagtcatg agcttcctct cgcccgaagt
    12481 ggccgcggtg ctgtgggccg tcgtgaaggc ggtcatcatc ctgctggccg cggtgatctt
    12541 cgccgcctac ctcagcttca tcgagcgccg cgtgctggcg ctgtggcagg accgctacgg
    12601 cccgaaccgc gtcggcccct tcggcatctt ccagctgccg gccgacgtgg tgaagatgct
    12661 gttcaaggag gactggatcc cgccgttcgc ggacaagctg acgttcctga tcgcgccgat
    12721 catcgcgatg agcgcgctgt tcatctccta catcatcgtc ccggtcacgc cgacgtggta
    12781 cgtggcggac ttcgacatcg gcatactgtt cttcctggcg atggcgggcc tggcggtcta
    12841 cgcggtgctg ttcgccggct ggtccagcaa caacaagtat tcgctgctcg gcggcctgcg
    12901 ctcggccgcg cagaccgtga gctacgaggt gttcatgggg ctgtcgctga tgggcgtggt
    12961 ggcgcagagc ggttcgttca acatgcgcga gatcgtcgag gcgcagcgcg atctgtggtt
    13021 catcgtgccg cagttcctcg gcttctgcac cttcgcgctc gcgggcatcg cggtcacgca
    13081 ccgcagcccc ttcgacctgc cggaagccga gcaggaactc gccgcgggct accacaccga
    13141 atacgccggg atgaagtggg cgatgttctt cgtcggtgaa tacatcggca tcgtggtggt
    13201 gtcctcgctg ctcacgacgc tgttcctcgg tggctggctc gggccgggcc ccgactggct
    13261 ggctccgttg tggttcgtgg cgaagacgct ggtattcgtg acgctgttca tactggttcg
    13321 cgccgcgctg ccccgcccgc gctacgacca cctgatgacg gcaggctgga tggtctgtct
    13381 cccgatcacg ctgctgaacc tgctggtgac cggcgccgtg ctgctggcca agggttgaac
    13441 acatgaacct gctcctgaaa atactcgcgg gcatcggcac caacctgcgc agcatgtgga
    13501 tgatcttctc gcgcgccacg cgcgcgcgtg ccacgatcca gtaccccgag caggccgtgt
    13561 acctgccccc gcgctaccgc gggcgcatcg tgctgacgcg cgacccggac ggcgaggagc
    13621 gctgtgtcgc ctgcaacctg tgcgccgtgg cgtgtcccgt gggctgcatc gcgctgcaga
    13681 aggccgagaa ggacgacggg cgctggtatc cggagttctt ccgcatcaat ttctcgcgct
    13741 gcatcttctg cggcatgtgc gaggaagcct gccccacgac cgcgatccag ctcacgccgg
    13801 atttcgagct ggccgagtac aggcgcgagg acctcgtgta cgagaaagag gacctgctga
    13861 tcgcgggcag cggcaagaac cccgaataca atttctaccg cgtcgcgggc atggcgatcg
    13921 cgggcaagcc caagggcgcc gcgcagggcg aggcccagcc gatcgacgtc agaacgctgc
    13981 tgccataggg ggtcgggaac gatggagctg actttctata cctgcgcgct ggtggcgatc
    14041 ctcgcgacgc tgcgcgtcgt gaccaacgcc aacccggtgc acgcgctgct ctacctcgtg
    14101 gtctcgctgc tggcggtggc gggcgtgttc ttcgcgctcg gggcgccctt cgcgggcgcg
    14161 ctcgaggtga tcgtctatgc cggcgccatc atggtgctgt tcgtgttcat cgtgatgatg
    14221 ctgaacctcg gacccgaaac gacggcgcag gagcggcaat ggctgaagcc ggcgacctgg
    14281 atcggcccga tgctgctgtc ggcgctgctg ctcggcgccc tggtgttcac gctggtcggc
    14341 gccgacgccc cgacgggcag cgcgacggtg ggcccgaagg cggtcggcat cgcgttgttc
    14401 ggcccctacg tgctcgtcgt cgagctcgcc tcgctgctgc tgctgggggc gctggtcgcg
    14461 gcgtaccacc tcggccgcga cgaccaccac tgagcgccgg gaacaaccaa tgatggaatc
    14521 gcgccatgat tgaagccgca tcggtccccc tggagcacgc cctcgcgctg gcggccgtgc
    14581 tgttctgcac cggcctcgcg ggcctgatgg tgcggcgcaa cttcctgttc atgctcatga
    14641 gcctcgaagt catgaccaac gccgcggcgc tcgccttcgt cgccgccggc agccgctggg
    14701 cgcagcccga cggccagatc atgttcatca tggtgctggc gctggcggcc gccgaggcct
    14761 cgatcgggct cgcgctgctg ctgcagctct atcgccgctt ccataccctc gatatcgacg
    14821 tcgccagcga gatgcgcgga tgagcttcct ctacctcact ttcctcgcgc cgctcgccgg
    14881 cttcctgctg ctcgccttct cgcgcgggcg cctcggcgag aacgccgccg ccatcatcgg
    14941 cgcgggctcg gtcggcgtga gcgcgctggt cacggcactc gcggccgcgc agttcagcgt
    15001 cccggtgacg caggtgctgt ggacctggat gcatatcggc gacttcgcgc cgcgcttcgc
    15061 cctttacctc gacgggctct cgctcacgat gctcggcgtg gtcaccgggg ttggcttcct
    15121 gatccacgtg ttcgcctcct ggtacatgcg cggcgaggag ggctactcgc gcttcttcgc
    15181 gtacatgaac ctcttcgtcg ccagcatggt gatgctggtg ctggccgaca acctcgcctt
    15241 cctctacctc ggctgggaag gcgtgggcat ctgctcctac ctgctgatcg gcttctacta
    15301 ccgcacggtc gccaacggca acgcggcgat caaggccttc ctggtcacgc gcgtgggcga
    15361 tgtgttcatg gccatcggcc tgttcatcct gctgtcgcaa ctcggcaccc tcgacatcca
    15421 ggaactgctc gcgcgcgcgc ccgagcgctt cgccagcggc gacccgctga tcgtcgcggc
    15481 cacgctgatg ctgctcggcg gcgcggtggg caagtccgcg cagctgccgc tgcagacctg
    15541 gctcgccgac gccatggccg gccccacgcc ggtatcggcg ctcatccacg ccgccaccat
    15601 ggtgaccgcc ggcgtgtacc tgatcgcgcg cacgcacggc ctgttcgagc tcgcgccgca
    15661 cgtgctggaa ctggtcggcg cggtgggcgc cgtgacgctg ctgatcgccg gcctgtgcgc
    15721 gctggtgcag accgacatca agcgcgtgct ggcctactcg accatgagcc agatcggcta
    15781 catgttcctc gcgctcggcg ccggcgcgta ttccgccgcg atcttccacc tgatgacgca
    15841 cgccttcttc aaggcgctgt tgttcctcgc cgccggcgcc gtgatcgtcg cctgccacca
    15901 cgagcagaac atcttccgca tgggcggcct gcgcaagcgc ctgccgctcg aatacgcgag
    15961 cttcctcgtg ggcggcggcg cgctgtgcgc cctgcccttc gtgaccgcgg gctttttctc
    16021 gaaggacgag atcctctggg ccgagtacac caccggacac cagttcctgt tcctggccgg
    16081 ggtggcaggc gcgttcctga cctctctcta caccttccgc ctgatcttca ccgtgttcca
    16141 cggcgagcag cacaccgagg cacacgcggg ccgcggtctt gcctacagcg tgccgctgat
    16201 cacgctgctc gtgctgtcca ccgggctcgg cgcgatgatc cacccgccgc tcgccgaggt
    16261 cctgccggtg ctgaagggac aggtcgcgga aggcgagcgc cacttcgtgg agatcctcgc
    16321 ggccggcgtc ggcatcgccg gtatcgcgct cgcggcagtg ctgttcctcg gcaagcgcac
    16381 gctggccacc gggctcgcga acagcgccgc aggacgcttc gtcggcaact ggtggctgaa
    16441 cgccttcggc ttcgatgcgc tgtacaacct gctgttcgtg cggccgttcc tgttcatcgc
    16501 ccgtcccggg cgccgcgacg cgctcgacgg cacgatcggc ctgctgccgg tcggtgtgcg
    16561 cgcgctgaac cgcctggtgg tgctgccgca gaccggcagg ctgcgctggt acgtggcgtc
    16621 gatagcgctg ggcgcggtgc tggtggtggg tttcgtcgtg atcaacctcg tctaaggaaa
    16681 cgctcggccc atgctgctcg cctggctgat actcatcccg atcgtaggcg gcctgctgtg
    16741 ctggcagacc gaccgcttcg gcaacctgcc gcgctggatc gcgctcggtt ccatgaccac
    16801 ggtcttcgcg ctgtcgctcg agctctgggc gaccggcaac tacagcctcg cgcacggcat
    16861 ccacgacgtg ccgcagtggc agatggaatt cgtgatgccg tggattccga gcctcggcat
    16921 cagtatccac ctcggcgtcg acggcctctc gctcgcgctc gtcgtgctca ccggcttcct
    16981 cggcatcctg tccgtgctgt gctcctggcg cgagatccag cgccgcaccg gcttcttcca
    17041 cctgaacctg atgtggatca tcggcggcgt ggtcggggtg ttcctcgcac tcgacctgtt
    17101 cctgttcttc ctgttct
//
LOCUS       DAORUQ010000058        16993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3092_length_16993_cov_6.316448, whole genome shotgun sequence.
ACCESSION   DAORUQ010000058 DAORUQ010000000
VERSION     DAORUQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_16993_cov_6.316448"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLW13_07055"
     CDS             <1..791
                     /locus_tag="PLW13_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY70167.1"
                     /translation="QRYLQLNDSGTPADEAFEQAYGERISGLERKLRRYARSKLHYMV
                     LRVKEPPAAVVMQHRSLSTAEAAAHIASLSLTGAEFDTAQRAAEAALALEPGNTLALA
                     VLGDVHKFGRRFDAAEPLYRKALELAPDDALRQLDYGEYWLDRARLEQDSAARGRLLA
                     EARRHFARAHAIDREIPELFAMNGASYLFPGEDLGKGVASLEAAYGMLPADEDIVMLL
                     AKAYTAAGRKADAAPLLRTIIARARSAQADEARRLLGEELAEAE"
     gene            complement(816..1409)
                     /locus_tag="PLW13_07060"
     CDS             complement(816..1409)
                     /locus_tag="PLW13_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70168.1"
                     /translation="MSDKPQSPTLTRAAMGITPGRALNVSPGGIEELRRDIQVLMDIE
                     AIKQVKHAYFRCIDTANWGEIGDYFHDDVTVHFIGGTYEWKLAGKQAYLDAVTQSFNR
                     QAIGHHNGHQPEIQILGETEATGIWYLADNMWQLDSRYFTTGTALYWDRYVKVDGRWL
                     IRETRYRRIYELNSVLEERPQPATHYLGDFGTPLRAS"
     gene            1598..2692
                     /gene="dinB"
                     /locus_tag="PLW13_07065"
     CDS             1598..2692
                     /gene="dinB"
                     /locus_tag="PLW13_07065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQY70169.1"
                     /translation="MQPVHVQRKIIHCDCDCFYASVEIRDDPSLAGLAVAVGGSPERR
                     GVITTCNYVAREYGVHSAMPSAHARRLCPGLVILPPDFDKYRAASRQIRQIFYDYTDL
                     VEPLSLDEAFLDVSAASACHGSATLIARDIRRRVRAEVGITISAGVAPNKFLAKVASD
                     WNKPDGDFVVLPGEVDAFVAALPVRRIFGVGKATAERLSGMGVSSCADLRAFSVFELT
                     QRFGRFGQRLYELCRGIDERAVQASQRRKSLSVERTYAEDLQGLEACQRQLPELLMTL
                     RSRLRRVSHEYLVTKLFVKLKFADFQSTTVECAARQPEPARVAELLGTALARSSLPVR
                     LLGIGVRFVDLREESNPAQLELFESDTGLT"
     gene            2779..3264
                     /locus_tag="PLW13_07070"
     CDS             2779..3264
                     /locus_tag="PLW13_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HQY70170.1"
                     /translation="MIKEVHGDILLSSAQAIAHGVSPNDNFAQGLALALREQWPALYK
                     DFRHFCQTAHPKSGEAWVWASAEGKRIVNLLTQEAAYGHGEKPGEATLPNVNHALRAL
                     RKLAETEKFSSIALPRLATGVGRLDWNDVRPLLDEHLGDLKVPVYVYTKYEKGVKAVE
                     A"
     gene            3323..3721
                     /locus_tag="PLW13_07075"
     CDS             3323..3721
                     /locus_tag="PLW13_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQY70171.1"
                     /translation="MAGPASVVTLRSAELEDIQSTAPPEFGGPAGNWSPETLFVAAIA
                     DCFILSFRAVANASKLEWSEIDCDAVGVLEKTAEGLWFTVIELDAHLRIPAGGDVARA
                     ERLLEKAEKSCLVSKSLRSEVRLQLRVSAG"
     gene            complement(3725..4201)
                     /locus_tag="PLW13_07080"
     CDS             complement(3725..4201)
                     /locus_tag="PLW13_07080"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HQY70172.1"
                     /translation="MSIDYHFTASVERVFALLTDADFLVDRCLALGELAADCTIEDDD
                     DEVVITLNREVERNLPAFLSRLFESRQNVEMVERWSRRGGARHGRFTLKVIGQPVTVT
                     AQMKLRADPAGGCTYTVQHTAKANVPLIGRRLESFILGQTEAGARAELDYLATALE"
     gene            complement(4246..5370)
                     /locus_tag="PLW13_07085"
     CDS             complement(4246..5370)
                     /locus_tag="PLW13_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQY70173.1"
                     /translation="MNQVVISGTGLFTPEQSISNSELVASFNAYAANWNAGHAAAIAA
                     GELAPMQESSEAFIEKASGIRSRFVIDKSGVLDPARMCPDIPERPDDQPSLQCEMAVH
                     AAREALAQAGRTAADIDAVIVACSNLQRPYPAVSVEVQAALGIGGFAFDMNVACSSAT
                     FGIDMARNAVLTGSARAVLVISPEICSGHLEFRDRDCHFIFGDACTAVVVEREAASGA
                     RAPFRIIDSRLKTTFSNNIRNNFGFLNRAAPEGIGARDKLFRQDGRKVFKEVCPMVAE
                     IIVRQLGDLGIPLEALRRLWLHQANLGMNQLIGRRVLGREPLPEETPVILDTYANTSS
                     AGSIIAFHQFRDDLARGERGVICSFGAGYSVGSLVVERCG"
     gene            complement(5572..6930)
                     /locus_tag="PLW13_07090"
     CDS             complement(5572..6930)
                     /locus_tag="PLW13_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQY70174.1"
                     /translation="MSGTLLINLAYALAAVLFIVGLKMLSSPASARKGNWLSSVAMLI
                     AVVVTLLDQQIVDFRWIAVGLLIGTAVGVLAARMVAMTSMPEMVALFNGFGGLASLLV
                     GWAALFTPNTTFILVTIALSILIGGVTFTGSVVAWAKLSETISGRPFLFSGQRIFNGA
                     LLAAIVASTVMFCLQPDNHLWLYAVIALSFLLGVMVVIPIGGADMPVVISLLNSYSGL
                     AACAAGFAINNMILIVAGALVGASGIILTQIMCKAMNRSLANVLFSGFGAVSSSDEKI
                     EGEVKAITHEDAYYVLEAASSVVIVPGYGMAVAQAQHTVKELQELLEKNGAEVVYAIH
                     PVAGRMPGHMNVLLAEADVSYDLLLEMDEVNPRIENFDVAIVIGANDVVNPAARESKG
                     SPIYGMPIINVDKARTVFVLKRSMASGFAGIDNPLFFKENTRMLFGDAKESISQLIRQ
                     FK"
     gene            complement(6933..7220)
                     /locus_tag="PLW13_07095"
     CDS             complement(6933..7220)
                     /locus_tag="PLW13_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQY70175.1"
                     /translation="MEPVYLTFILMLSIFLGFELINKVPATLHTPLMSASNAISGITL
                     VGALVSAGAEHGTLAVVLGTAAVALATINVVGGYVVTDRMLGMFKKREGGK"
     gene            complement(7232..8365)
                     /locus_tag="PLW13_07100"
     CDS             complement(7232..8365)
                     /locus_tag="PLW13_07100"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HQY70176.1"
                     /translation="MVIGIPRELQPGEQRAAMIPASIQKLVRAGAQVVIESGLGLGAG
                     HSDEDYATAGAHVTHDRAALLAQSELVLRLHKPPVAEIAQLKRGAIHISYLDPFNEPG
                     LIDAFVSAGVTAISMEMIPRTTRSQKMDALSSQGNLAGYVMVLLAAARLPRILPMMMT
                     PAGTLKPANVFVIGAGVAGLQAIATAKRLGAKVVAFDTRSVVAEQVQSVGAKFLEIDL
                     GETGQTKDGYAVQLTPEQLEKQKQGQAKQISESDLVITTAQVFGRKPPVLVTRDMLAG
                     MRPGSVIVDMAAETGGNVEGSVAGETVVVNGVTIIGNGNWANCVPRDASQMYSSNLLN
                     LIEEFWHKEDKRLALNFEDDILQGCVITHEGMIVNQTIKNLKR"
     gene            complement(8511..10154)
                     /locus_tag="PLW13_07105"
     CDS             complement(8511..10154)
                     /locus_tag="PLW13_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQY70177.1"
                     /translation="MSPNPGSRRTRIRAACTLLGALAAGAPPLLAGEWQLDTLVPGSP
                     FRSLHGLAFGPDARIYAGSVVGQSIHRVDPDTGASEIFIAPPAGLADDIEFAPDGTMY
                     WTGFTHGTMNALSPGGEPRVIASGLPGLNSLALDARGRLFGTQVFLGDALWEFDPEGK
                     APPRLVKKDLGGLNGFDFGPDGRLCGPLWFRKQVVCLDVDSGAMEVVAEGLVQPAAAN
                     FNSRGELFAIDNETGEIFRIDIAAHRRERVAQAPTNLDNLAFDAADRLYVTNMSDNAI
                     YAVDPAGGDVRRVTGGALSLPGGIAAADGTLYVADAFTLRAVDLAGGAVRDISRSLAQ
                     TGYPSAVAAANGRIATASHEAGGVQLRDAATLKILAQWTGLAQPSALALLDADRLAVT
                     EAGGGGRLLVLDRHDSAARHVVAEGLQQPMGLLRSAEGYIVSESAAGHLTAISDAGER
                     RVIASGLARPEGIARLDDGTLAVAETGTRRLLLIDPADGSATVIATDLPLGIAGAEEQ
                     PPGVPTGVALGTDGTIYLSADRDGSILRLRRVAGPGQGE"
     gene            10403..10786
                     /locus_tag="PLW13_07110"
     CDS             10403..10786
                     /locus_tag="PLW13_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HQY70178.1"
                     /translation="MVIRVMLGLLGLMWAGYGAWCFADPGMLRDVAGISASRASGSVD
                     LRATYGGLQMAVGVLLLGGALRAAMTRQVLLVYGVLCAGIGLARLLAALLESEWSGYT
                     VGAICFELGSAAAALVLFSRGGARV"
     gene            10779..12260
                     /gene="pap"
                     /locus_tag="PLW13_07115"
     CDS             10779..12260
                     /gene="pap"
                     /locus_tag="PLW13_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HQY70179.1"
                     /translation="MFEAAEIGHRISKARYRREEPALRQALLEAQYRLLDKPEFPLLI
                     LIGGLDGAGKGETVNLLNEWMDPRHIRTEAFGLSGSAWEGGRPEMWRFWQVLPPRGKI
                     GVFFGSWYTDPLVRRAEGMMKRTAFERRLETIRHFERMLVAEGALVLKLWFHLSKSAQ
                     KKKLRELAEDPLTAWRVTPEEWKRHDRYERYVKVIAETLRETGTGEAPWQVIEGSDER
                     YRALTAGRAVLDALTRRLDGPAPVIAPSAPPPVPAIDGRDLLNSLDYSRRIAAKNYDR
                     ELERAQGRLNLLTRHKRMRERSLVLAFEGMDAAGKGSTIRRVTRALDARYYRVVPVGA
                     PTDEERAQPYLWRFWRHLPADGKAVIFDRSWYGRVLVERVEGYCAEADWMRAYHEINE
                     FEQELVAGGAIVAKFWLSVTLAEQLKRFRAREQTPYKQHKITAEDWRNREKWPAYEQA
                     VCDMVERTSTSYAPWFLVPANDKRYARIAVLKHIADEIEKHIA"
     gene            complement(12360..13793)
                     /locus_tag="PLW13_07120"
     CDS             complement(12360..13793)
                     /locus_tag="PLW13_07120"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HQY70180.1"
                     /translation="MGAEPLQQFSAANAELQATLALQREAYLAHPVPALAERQGDLRK
                     LQRFVRENKEAIITAICADYGNRSRHETLFAEIFTAIDGVDHALKHLKHWMKPQKRPV
                     DRRNFLGAKNRVIPQPLGVVGVIVPWNFPLNLSILPLIYIFAAGNRAMVKMSENSRHL
                     ARLMIEKMPAYFPPDKLAVFEETGGVGVEFSRLPFDHLLFTGSSQTGRSVMAAAAQNL
                     CPVTLELGGKSPAIVCDDYPLAKAAERILFVKLFNAGQICTTVDYVFLPEAKVDAFVA
                     LASRIVPARYPRLDSPDFTALIDERAFRRVTAALAEAEARGATLVQLVPGQRWDEATR
                     KIAPHLVLNAPDDCELLTREIFGPVLPVRGYRSLEEVIGYVNARPRPLALYPFSNDAA
                     RVQLLLDRVMSGGVSVNDALFHVAQHDLPFGGVGASGMGHYHGHEGFLTFSKLRPVFY
                     QASFSAMKFLAPPYGRFANTLLNFLTR"
     gene            13923..16010
                     /locus_tag="PLW13_07125"
     CDS             13923..16010
                     /locus_tag="PLW13_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY70181.1"
                     /translation="MNSVVDYAVEAGVAVITVDSPPVNALSAPVRAGVYRGVETATTD
                     AAVGAIVLICAGRTFCAGADITEFGKPPVSPLLQDLQSLIENAPKPVVAAIHGTALGG
                     GLELALVAHYRIAVPAASCGLPEVNIGLIPGAGGSQRLPRVVGVEAALEMITSARHVP
                     AAQAHAMGLLDVLAQGPDLRAAAVAFARRVMAEGRPLRKVRELDAKLQAARAAPGLFA
                     EFRAGHARQFRGFLAPEYAIRAVEAAVNLPFDAGIAEEKRLFRELLEGSQSAAQRHAF
                     FAERRAAKVPGVPADMPTRTIARVGVIGAGTMGGGIAMNFLSAGIPVVLVETSREALD
                     RGIGVIRRNYENSAKRGASGAADVARNMALLTGSLALGDLADCDLVIEAVFEQMAIKQ
                     DVFARLDRVAKPGAILASNTSYLDIDAIAASTSRPGDVLGLHFFSPANIMRLLEIVRG
                     RDTAVDVIATAMKLARTIAKVGVVVGNCHGFVGNRMLAARNREADRLVLEGALPWDVD
                     RVLYEFGFPMGPFRMRDLAGLDIGWDAAKSSSSTVRELLCEMGRRGQKTGAGYYDHHA
                     ARNAAPSAVTERLILDFAARQGVARRAIADEEILERCLYPMVNEGAKILEEGIAARAS
                     DIDTVWLCGYGWPRYRGGPMFWAELEGLPKILGRLRALQQVHGDEFRPAPLLERLVAQ
                     GGSFHDFAGPDAG"
     gene            complement(16044..16877)
                     /locus_tag="PLW13_07130"
     CDS             complement(16044..16877)
                     /locus_tag="PLW13_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HQY70182.1"
                     /translation="MEQLRTWIGREEERHDLLGEGLLARFRATLESRVAESEGIAPRG
                     VHWCLCTPEDAMSALGGDGHPRTGGFLPPVPLPRRMWTSGSVDFLAPLRSGAAIRRRS
                     RVAAVSEKRSRSGATVFVELDHLTFADDAPAVRERQTLVWREAAAGMQALPGSGGADL
                     SDWEFTRSRIPSAPLLFRYSALTFNAHRIHYDLPYAQHDEGYPALVVQAPLLATLLLG
                     HAATRGNPSDPARFSFRAVAPAYAGQLLHLAGRETGGGTDLAAIDNDGRRLMEARLEP
                     R"
BASE COUNT         2596 a         5630 c         6009 g         2758 t
ORIGIN      
        1 cgcagcgcta cctccaactc aacgactccg gcacgccggc cgacgaggcc ttcgagcagg
       61 cctacggcga gcggatcagc ggactcgagc ggaaactgcg ccgctacgcg cgcagcaagc
      121 tccattacat ggtcttgcgg gtcaaggagc cgccggccgc ggtggtcatg cagcaccgct
      181 ccttgtcgac cgccgaggcg gcggcacaca tcgcctcgct cagcctcacg ggtgccgagt
      241 tcgacaccgc ccagcgcgcg gccgaggccg ccctggcgct ggaaccgggc aacaccctgg
      301 cgctcgccgt gctcggtgac gtgcacaagt tcgggcgtcg cttcgacgcg gcagagcccc
      361 tgtacagaaa ggcgctggag ttggcgccgg acgacgccct gcgccagctc gactacggcg
      421 agtactggct ggaccgcgcg cggctcgagc aggattccgc ggcgcgcggc cgcctgctgg
      481 cggaggcgcg gcgccatttc gcccgcgcac acgcgatcga ccgggaaatt cccgagctct
      541 tcgccatgaa cggagcgtcc tacctgtttc cgggcgagga cctcggcaag ggcgtcgcga
      601 gcctggaggc ggcctacggg atgctgccgg cggacgagga catcgtgatg ctgctggcga
      661 aggcctacac ggcggcaggc cggaaggcgg atgcggcgcc actcttgcgc accatcatcg
      721 cccgggcgcg cagtgcccag gccgatgagg cgcgccgcct gctgggagag gagctcgcgg
      781 aggcggagtg agcccgcgcg gcgcgcgggc ccggctcagg acgcccgcag cggcgtgccg
      841 aagtctccga ggtagtgcgt ggcgggctgc gggcgctcct cgagcacgga gttcagttcg
      901 tagatgcgac ggtagcgcgt ctcgcggatc agccaacggc cgtcgacctt cacgtagcgg
      961 tcccagtaca gcgcggtgcc ggtggtgaag tagcgcgaat cgagctgcca catgttgtcg
     1021 gccaggtacc agatgccggt ggcctcggtt tcgcccagga tctgtatttc gggctggtgg
     1081 ccgttgtggt ggccgatcgc ctggcggttg aaggactggg tgaccgcgtc caggtaggcc
     1141 tgcttgcccg cgagcttcca ctcgtaggtg ccgccgatga aatgcaccgt cacgtcgtcg
     1201 tggaagtagt cgccgatctc gccccagttc gccgtgtcga tgcaacggaa ataggcgtgc
     1261 ttcacctgct tgatggcctc gatgtccatc aggacctgga tgtcgcggcg cagttcctcg
     1321 atgccgcccg ggctcacgtt cagcgcccgg cccggcgtga tgcccatggc ggcgcgggtg
     1381 agggtggggg attggggttt gtcgctcatc ttcgcgtcct cgattgtctt gtttatgggc
     1441 cgggttcgtc atgttccggc cgtcgatcat aagcgagcgg gcgcgcccgt gcgagccatt
     1501 gcgccagcgc gatttcgccg cgcttggccg tcgccggtcc attgctgcga taatgcgaca
     1561 ccacgttcgc caggtgtcgc cgcaaccgcg gtttcgcatg cagccagtcc acgtccagcg
     1621 caagatcatc cactgcgatt gcgactgctt ctacgcgtcg gtcgagatcc gcgacgaccc
     1681 ctcgctggcg ggcctggcgg ttgcggtcgg cggcagtccc gagcgccgcg gcgtgatcac
     1741 cacctgcaac tacgtggcgc gcgaatacgg cgtgcattcg gcgatgccct cggcgcatgc
     1801 gcgccggctc tgcccggggc tggtgatcct gccgccggat ttcgacaagt accgcgccgc
     1861 ctcgcgccag atccgccaga tcttctacga ctacaccgac ctggtggagc cgctgtcgct
     1921 ggacgaagcc ttcctcgacg tgagcgcggc gagcgcctgc catggcagcg ccacgctgat
     1981 cgcgcgcgac atccgtcggc gcgtgcgcgc cgaggtcggc atcacgatct ccgcgggggt
     2041 ggcgccgaac aagttcctgg ccaaggtggc cagcgactgg aacaagcccg acggcgattt
     2101 cgtggtgctg cccggggagg tcgatgcctt cgtcgcggcg ctgccggtgc gacgcatctt
     2161 cggcgtgggc aaggcgaccg ccgagcgcct cagcggcatg ggggtgtcga gctgcgccga
     2221 tctgcgcgcg ttctccgtgt tcgagctgac gcagcgcttc gggcgcttcg gccaacgcct
     2281 gtacgagctg tgccgcggca tcgacgagcg cgcggtgcag gcgagccagc ggcgcaagtc
     2341 gctgagtgtg gagcgcacct acgccgagga cctgcagggg ctcgaggcct gccagcgaca
     2401 gttgccggag ctgctgatga cgttgcgctc gcgcctgcgg cgcgtttcgc acgagtacct
     2461 ggtgaccaag ctgttcgtga agctcaagtt cgccgacttc cagagcacga cggtggagtg
     2521 cgcggcgcgc cagccggagc cggcgcgcgt ggcggagctg ctgggcacgg cgctggcgcg
     2581 cagctccctg ccggtgcggc tgctcgggat cggggtgcgc ttcgtggacc tgcgcgagga
     2641 gagcaacccg gcgcagctcg aactgttcga aagcgatacc ggcctgacct gaatcaagcg
     2701 tcgcggcgcc atctggcgta tgatgcgcgc ggtttccgga cgggtcgtcc ggcacagggt
     2761 ttcattttcg gaggcatcat gatcaaggaa gtacacgggg atatcctgct gagttctgcg
     2821 caagccatcg cgcacggggt gtcgcccaac gacaacttcg cccagggcct cgccctcgcc
     2881 ctgcgcgagc agtggccggc gctgtacaag gatttccgcc atttctgcca gaccgcgcac
     2941 ccgaagtcgg gcgaggcgtg ggtctgggca tcggccgagg gcaagcgcat cgtgaacctg
     3001 ctgacgcagg aagccgccta tggtcatggc gaaaagccgg gcgaagcgac gctgcccaac
     3061 gtcaatcatg cgctgcgcgc gctgcgcaag ctcgccgaga ccgagaagtt cagcagcatc
     3121 gcactgccgc gcctggcgac cggcgtgggc cgactggact ggaacgacgt gcgcccgctg
     3181 ctcgacgagc atctgggcga cctgaaggtt ccggtctatg tctacacgaa atacgaaaag
     3241 ggcgtgaagg ccgtcgaggc ctgagcgcag atcgaggtac agccagatgc acccatttcc
     3301 tcaccgttac gcggtaagcg ccatggctgg cccggcgagc gtggtgacgc tgcgcagcgc
     3361 cgagctcgag gacatccaga gcaccgcgcc gccggagttc ggcggccccg cgggtaactg
     3421 gtcgcccgag acgctgttcg tcgcggcgat cgccgactgc ttcattctca gcttccgcgc
     3481 ggtggccaac gcctccaagc tcgagtggag cgagatcgac tgcgatgcag tcggtgtgct
     3541 ggagaagacg gcggaggggc tgtggttcac cgtgatcgag ctcgacgctc acctgcgcat
     3601 tcccgccggc ggtgacgtcg cgcgggccga gcgcctgctg gaaaaggccg agaagagctg
     3661 cctggtgtcg aagtcgctgc gatccgaggt acggctgcag ctccgggtga gtgccggctg
     3721 aacgctattc gagggcggtg gcgaggtagt cgagttctgc ccgcgcaccg gcctcggtct
     3781 gccccaggat gaagctctcg aggcgccggc cgatcagcgg cacgttcgct ttcgcggtgt
     3841 gctgcacggt gtaggtgcag ccgcctgccg gatcggcgcg cagcttcatt tgcgccgtca
     3901 ccgtcaccgg ctggccgatc accttcagcg tgaagcggcc gtggcgcgcc ccgccgcgcc
     3961 ggctccagcg ctcgaccatc tccacgttct gccggctctc gaacagccgc gacaggaagg
     4021 cgggcaggtt gcgctccacc tcgcggttca gtgtgatcac cacctcgtcg tcgtcgtcct
     4081 cgatcgtgca gtcggcggcg agctcgccga gcgcgaggca gcggtcgacc aggaaatccg
     4141 cgtcggtcag cagcgcgaac acgcgttcga cgctggccgt gaagtggtag tcgatgctca
     4201 tggcggcagg tctcccgtgg cggttcaggt cacgattcca ttgcttcagc cgcagcgctc
     4261 gaccaccagg ctgcccaccg agtagcccgc cccgaaggag cagatcacgc cgcgctcgcc
     4321 ccgggcgaga tcgtcgcgga actggtggaa cgcgatgatc gagcccgcgg aactggtgtt
     4381 ggcgtaggtg tcgagaatca ccggcgtctc ctccggcagg ggttcgcgcc cgagcacgcg
     4441 ccggccgatc agctggttca tgccgaggtt ggcctggtgc agccacagcc ggcgcagtgc
     4501 ctcgagcgga atcccgagat cgccgagctg gcgcacgatg atctcggcca ccatcgggca
     4561 gacttccttg aacaccttgc gcccgtcctg gcggaacagc ttgtcgcgcg cgccgatacc
     4621 ttcgggggca gcgcggttca ggaagccgaa gttgttgcgg atgttgttcg agaaggtggt
     4681 cttcaggcgc gagtcgatga tgcgaaacgg cgcgcgcgcg ccgctcgccg cctcgcgctc
     4741 caccaccacc gcggtgcagg catcgccgaa gatgaagtgg cagtcgcggt cgcggaactc
     4801 caggtgcccg gagcagatct cggggctgat caccagcacc gcgcgcgccg agccggtgag
     4861 caccgcgttg cgcgccatgt cgatgccgaa ggtggccgag gagcaggcga cgttcatgtc
     4921 gaaggcgaag cccccgatgc ccagcgccgc ctgcacctcg acgctgacgg ccgggtaggg
     4981 gcgctgcagg ttcgagcagg cgacgatgac cgcgtcgatg tcggcggcgg tgcgcccggc
     5041 ctgcgccagc gcctcgcgcg ccgcgtgcac ggccatctcg cactgcagcg agggctggtc
     5101 gtccgggcgc tcggggatgt cggggcacat gcgcgcgggg tcgagcacgc cgctcttgtc
     5161 gatcacgaag cggctccgga tgcccgaggc cttctcgatg aaggcctcgc tggattcctg
     5221 catcggcgcg agctcgccgg cggcgatggc cgcggcgtgc ccggcattcc agttcgcggc
     5281 gtaggcgttg aacgaggcca ccagctcgct gttggaaatc gactgctccg gggtgaacag
     5341 tcccgttccg ctgatcacga cctgattcat gtgtgggatc cctgcaagag cggcttgatg
     5401 ccgcaattgc ggggattgta cgggcgcaga ccctgcgcgg gccagtaggc cggcattcat
     5461 gcaggtcggg attcatgccg aaaaccgccc ccggcggatc ctgcgggttc ggcgtcgccg
     5521 aacccttgtc gggatgaatc ccggcctacg tcgcccgacc gaaggccggg ttcatttgaa
     5581 ttgccggatc agctggctga tcgactcctt ggcatcaccg aacagcatcc gcgtgttctc
     5641 cttgaagaac agcgggttgt cgatgccggc gaaaccggag gccatggaac gcttgagcac
     5701 gaacaccgtg cgcgccttgt cgacgttgat gatcggcatg ccgtagatcg ggctgccctt
     5761 ggactcgcgc gccgccggat tcaccacgtc gttggcgccg atcacgatcg ccacgtcgaa
     5821 gttctcgatg cgcgggttga cctcgtccat ctccagcagc aggtcgtagg agacatcggc
     5881 ctcggcgagc agcacgttca tgtgcccggg catgcgtccc gccaccgggt ggatcgcgta
     5941 gaccacctcg gcgccgttct tttccagcag ttcctgcagt tccttgacgg tgtgctgcgc
     6001 ctgcgccacg gccatgccgt agccaggcac gatcaccacg ctggaggccg cctcgaggac
     6061 gtaataggca tcctcgtgcg tgatcgcctt gacctcgccc tcgatcttct cgtcggagga
     6121 gctcaccgca ccgaagccgc tgaacaacac gttggcgagc gaacggttca tcgccttgca
     6181 catgatctgc gtgaggatga tgccgctcgc gcccaccagc gcgccggcga cgatcaggat
     6241 catgttgttg atcgcgaagc cggccgcgca ggccgcgagg cccgagtagc tgttcagcag
     6301 cgagatcacc accggcatgt cggcgccgcc gatcgggatg accaccatca cgccgagcag
     6361 gaatgacagc gcgatgaccg cgtacagcca caggtggttg tccggctgca ggcagaacat
     6421 cacggtactc gccacgatgg ccgcgagcag cgcgccgttg aatatgcgct ggccgctgaa
     6481 caggaagggc cgcccgctga tggtctcgct cagctttgcc cacgcgacca cgctgccggt
     6541 gaaggtcacg ccgccgatca ggatcgacag tgcaatcgtg accaggatga aggtcgtgtt
     6601 cggcgtgaac agcgcggccc agcccaccag caggctggcg aggccgccga agccgttgaa
     6661 caacgcgacc atctcgggca tcgaggtcat cgcgaccatg cgcgcggcga gcacgcccac
     6721 cgcggtgccg atcaggaggc ccacggcgat ccagcggaaa tcgacgatct gctggtcgag
     6781 cagcgtgacc accactgcga tcagcatcgc gaccgaggat agccagttac ccttgcgcgc
     6841 cgaagccggc gagctcagca ttttcaggcc gacgatgaag agtaccgccg ccagcgcgta
     6901 agccaggttg atgaggagtg tgccgctcat gattacttac cgccttccct tttcttgaac
     6961 atgccgagca tgcggtcggt gaccacgtac ccgccgacga cgttgatcgt ggccagcgcc
     7021 acggcggctg tgccgagcac gaccgcgagc gtgccgtgct ccgcgccggc cgacaccagt
     7081 gcgcccacca gcgtgatgcc cgagatcgcg ttcgacgccg acatcagcgg cgtgtgcagc
     7141 gtggcgggca ccttgttgat cagctcgaag ccgagaaata tcgacagcat caggatgaat
     7201 gtcaggtata cgggttccat ggcggcggcc ctcagcgctt caggttcttg atggtctggt
     7261 tcacgatcat gccctcatgg gtgatcacgc aaccctgcag gatgtcgtcc tcgaagttca
     7321 gcgccaggcg cttatcctcc ttgtgccaga actcctcgat caggttcagc aggttcgagg
     7381 aatacatctg gctggcatcg cgcggcacgc agttcgccca gttgccgttg ccgatgatcg
     7441 tcacgccgtt caccaccacg gtctcgccgg caaccgagcc ctcgacgttg ccgccggtct
     7501 cggccgccat gtcgacgatc acgctgcccg ggcgcatgcc cgcaagcatg tcgcgcgtga
     7561 ccagcaccgg cggtttgcgc ccgaagacct gcgccgtggt gatcacgagg tcggactcgg
     7621 aaatctgctt cgcctggccc tgcttctgct tctcgagctg ctcgggcgtg agctgcaccg
     7681 cgtagccgtc cttggtctgg ccggtctcgc cgaggtcgat ctcgaggaac ttggcgccca
     7741 ccgactgcac ctgctcggca accaccgagc gcgtgtcgaa ggccacgacc ttggcgccca
     7801 ggcgcttcgc cgtcgcgatg gcctgcaggc cagcgacacc ggcgccgatc acgaacacgt
     7861 tggcgggctt cagcgtgccg gcgggcgtca tcatcatggg caggatgcgc ggcaggcgcg
     7921 cggcggccag cagcaccatc acgtagcccg cgaggttgcc ctgcgaactg agcgcatcca
     7981 ttttctgcga gcgcgtggtg cgcgggatca tctccatgct gatcgcggtc acgccggcgc
     8041 tgacgaatgc atcgatcagg ccgggctcgt tgaacggatc gaggtaactg atgtggatcg
     8101 cgccgcgttt gagctgcgcg atctccgcga ccgggggctt gtgcaggcgc agcaccagct
     8161 ccgattgcgc cagcagcgcc gcgcggtcgt gcgtgacgtg tgccccggcc gttgcgtaat
     8221 cctcgtcgga atgaccggcg ccgaggccga ggcccgactc gatcacgacc tgcgcgccgg
     8281 cgcggaccag cttctggatg ctcgccggaa tcatcgcggc gcgctgctct cccggctgga
     8341 gctcgcgtgg aattccgatc accatgctcg ttctcctgtt gcatgttctg tccggccccg
     8401 gtcgtggcgc gaagccgaag cccggggccg ggcgattata tgtgccttgc gatcgtcagg
     8461 cgcttcatac atttgcacga tgtgcatgac aaacgctaat gatgccgtcg ttattctcct
     8521 tgtcctggcc cggccacgcg cctcaggcgc aggatgctgc cgtcgcgatc cgcgctgagg
     8581 tagatcgtgc cgtccgtgcc cagcgccacg ccggtcggaa caccgggcgg ctgctcctct
     8641 gcgcccgcga tgccgagcgg cagatccgtt gcgatcaccg tggccgagcc atcggccgga
     8701 tcgatcagca gcaggcgccg ggtaccggtc tccgcgaccg cgagcgtgcc gtcgtcgagc
     8761 cgcgcgatcc cctcggggcg cgcgagtccg ctcgctatca cgcggcgctc gccggcatcg
     8821 ctgatggccg tgaggtgtcc cgcggcgctt tcgctcacga tatagccctc cgcggagcgc
     8881 agcaggccca tgggctgctg cagtccttct gctaccacgt gccgtgctgc gctgtcgtgc
     8941 cggtcgagca cgagcaggcg cccgcccccg ccggcctcgg tcaccgcgag ccggtcggcg
     9001 tcgagcagcg cgagcgccga cggctgggcc agcccggtcc actgcgcgag tattttcagc
     9061 gtggcggcat cgcgcagctg cacgccgccg gcctcgtggc tggcggtcgc gatccgcccg
     9121 ttggcggcgg caacggcgct cgggtagccg gtttgcgcca gcgagcggct gatgtcgcgc
     9181 accgcgccgc cggcgaggtc cacggcgcgc agcgtgaagg catcggcgac gtagagcgtg
     9241 ccgtccgccg ccgcgatgcc gccgggcagg ctgagtgcgc cgcccgtcac ccggcgcacg
     9301 tcaccgccgg cggggtcgac cgcgtagatc gcgttgtcgg acatgttggt gacgtagagg
     9361 cggtccgcgg catcgaaagc gaggttgtcg agattcgtcg gtgcctgcgc cacgcgctcg
     9421 cgccggtgcg cggcgatgtc gatgcggaag atctcgccgg tttcgttgtc gatcgcgaac
     9481 agctcgccgc gtgaattgaa attcgcggcc gcgggttgca cgaggccttc ggccacgacc
     9541 tccatggcgc cgctgtcgac gtcgaggcag accacctgct tgcggaacca cagcgggccg
     9601 cacaggcggc cgtcggggcc gaaatcgaag ccgttcagtc cgccaaggtc tttctttacg
     9661 agccgtggcg gcgccttgcc ctcgggatcg aattcccaca gcgcatcgcc caggaacacc
     9721 tgcgtgccga acaggcgccc gcgcgcgtcc agcgccagcg aattgagtcc cggcaggccg
     9781 gaggcgatca cgcgcggctc gccgcccggg ctgagcgcgt tcatggtccc gtgcgtgaaa
     9841 ccggtccagt acatggtgcc gtcgggcgcg aattcgatgt cgtcagccag tccggcgggg
     9901 ggagcaatga agatctcgct cgcgcccgtg tccggatcga cgcggtgaat tgactgtccc
     9961 accacgctgc cggcgtagat cctggcgtcg ggcccgaagg ccaggccgtg gaggctgcga
    10021 aacggcgaac cgggaaccag ggtgtcgagc tgccactccc cggccagtag cggcggcgcg
    10081 ccagccgcca gcgcgcccag cagggtgcag gcggcgcgga tgcgcgtgcg gcgactgcct
    10141 gggttcgggc tcatgagagg ggtcctgtga gcgaggcgct gttatggtcg tgatggtcgc
    10201 gcgcgctcga acgcgctgcg gggcaagctt aggggcgatc gcgcccgggc gaaagccccc
    10261 ggggtctaaa tgcgacgcgg gagcgggcgc gtgacgccgc aggcgccgcg atagcccccg
    10321 cgcgcagcgc cgtgcttaaa tgcaaagcag gtcagtatcc gcagtggctg gggcaacagg
    10381 cggtggcgag gagaccggtg tcatggtgat tcgggtgatg ctcgggctgc tgggcctgat
    10441 gtgggcgggc tacggggcgt ggtgcttcgc cgatccgggg atgttgcgcg atgtggccgg
    10501 aatatccgcc tcgcgcgcga gcggcagcgt cgacctgcgc gccacttatg gcggactgca
    10561 gatggccgtg ggggtgctgc tgctcggcgg tgcgttgcgc gcggcgatga cgcgccaggt
    10621 gctgcttgtc tacggcgtgc tctgcgcggg catcgggctg gcgcgcctgc tcgcggcgtt
    10681 gctcgagagc gagtggtccg gctacaccgt gggggcgatc tgtttcgagc tcggcagcgc
    10741 cgctgccgcg ctggtgctgt tcagccgtgg aggtgcgcgt gtttgaagcg gctgaaatag
    10801 gtcatcgcat cagcaaggcg cgctaccgcc gcgaggagcc ggcgctgcgc caggcgctcc
    10861 tggaggcgca ataccggctg ctcgacaagc ccgagtttcc actgctgatc cttatcggcg
    10921 gcctggacgg cgcgggcaag ggcgagacgg tgaacctgct gaacgaatgg atggatccgc
    10981 gccatatccg caccgaggct ttcgggctgt ccggaagcgc ctgggagggc gggcgtccgg
    11041 agatgtggcg tttctggcag gtgctgccgc cacgcggcaa gatcggggtc ttcttcggct
    11101 cgtggtacac cgatccgctc gtgcgccggg ccgaaggcat gatgaagcgc accgccttcg
    11161 agcgccggct ggagaccatc cgccacttcg agcgcatgct ggtggccgag ggcgcgctgg
    11221 tgctgaagct ctggttccac ctgtcgaagt ccgcgcagaa gaagaagctg cgcgagctgg
    11281 ccgaggatcc gctcacggcc tggcgggtca cgcccgagga gtggaagcgc cacgacaggt
    11341 acgagcgtta cgtgaaggtc atcgcggaga ccctgcgcga gaccggcacg ggcgaggcgc
    11401 cgtggcaggt gatcgagggt tccgacgaac gctatcgcgc gctcacggca ggccgcgcgg
    11461 tgctcgatgc gctcacgcgc cggctcgacg gaccggcacc ggtgatagcg ccctcggcac
    11521 cgcctccggt gccggccata gacggccgcg atctgctgaa ctcgctcgat tactcgcgca
    11581 ggatcgccgc gaagaactac gaccgggagc tcgagcgcgc gcagggccgg ctgaacctgc
    11641 tgacgcgcca caagcgcatg cgcgagcgct ccctggtgct ggccttcgag ggcatggacg
    11701 cggcaggcaa gggcagcacc atccgtcgcg tgacgcgcgc gctggatgcg cgctactacc
    11761 gcgtggtgcc ggtgggcgcg cccaccgacg aggagcgcgc gcagccctac ctctggcgtt
    11821 tctggcgcca cctgccggcc gacggcaagg ccgtgatctt cgaccgttct tggtacggcc
    11881 gcgtgctggt ggagcgcgtc gagggctact gcgccgaggc ggactggatg cgggcctatc
    11941 acgagatcaa cgagttcgag caggaactgg tggcgggcgg ggcgatcgtg gcgaagttct
    12001 ggctgtcggt cacgctggcc gagcaactga agcgtttccg cgcccgcgag cagactccct
    12061 acaagcagca caagatcacg gcggaggact ggcgcaaccg cgagaaatgg cccgcttacg
    12121 agcaggcggt ctgtgacatg gtcgaacgca catcgaccag ttacgcgccg tggttcctgg
    12181 tgcccgcgaa cgacaagcgc tatgcgcgga tcgcggtgct gaagcacatc gccgacgaga
    12241 tcgagaagca catcgcctag gtccgcattc atgcggacag accggcgtag gtccgcattc
    12301 atgcggacaa ttgtccggct gaagccggac ctacgcgaac cgcgaaccgc gagccgcgat
    12361 cagcgggtca ggaaattcag cagcgtgttc gcgaaccggc cgtagggtgg tgccaggaac
    12421 ttcatcgcgc tgaacgatgc ctggtagaac accgggcgca gcttagagaa cgtcaggaag
    12481 ccctcgtggc cgtggtaatg gcccatgccg ctcgcgccca ccccgccgaa gggcaggtcg
    12541 tgctgcgcga cgtggaacag cgcgtcgttg acactcacgc cacccgacat cacgcgatcc
    12601 agcagcagtt gcacgcgcgc cgcgtcgttg ctgaacgggt agagcgccag cggccgcggc
    12661 cgcgcgttca catacccgat cacctcctcg agcgagcggt agccgcgcac cggcagcacc
    12721 ggaccgaaga tctcgcgcgt gaggagctcg cagtcgtccg gggcgttcag caccaggtgc
    12781 ggcgcgatct tgcgcgtggc ctcgtcccag cgttggcccg gcaccagttg caccagcgtg
    12841 gcgccgcggg cctcggcctc ggccagcgcc gcggtgacgc ggcggaatgc gcgctcgtcg
    12901 atcagggcgg tgaagtcggg cgaatcgaga cgcgggtagc gcgccggcac gatccggctc
    12961 gccagcgcca cgaaggcatc caccttcgcc tcgggcagga agacgtagtc caccgtggtg
    13021 cagatctggc cggcgttgaa cagtttcacg aacaggatgc gttcggcggc ttttgccagc
    13081 gggtagtcgt cgcagacgat ggccggcgac ttgccgccga gctccagcgt gaccgggcag
    13141 aggttctgcg ccgcggcggc catcacgctg cgcccggtct ggctcgaacc ggtgaacagc
    13201 aggtggtcga agggcaggcg cgagaactcg acgcccacac cgccggtctc ctcgaagacc
    13261 gcgagcttgt ccggcgggaa ataggccggc atcttctcga tcatgagccg cgcgaggtgg
    13321 cgcgagttct ccgacatctt gaccatcgcg cggttgcccg cggcaaatat gtagatcagg
    13381 ggcaggatgc tcaggttgag cggaaagttc cacggcacga tcaccccgac cacgccgagc
    13441 ggctgcggga tgacgcggtt cttcgcgccc aggaagttgc ggcggtccac cgggcgtttc
    13501 tgcggcttca tccagtgctt caggtgcttc aatgcgtggt cgacaccgtc gatcgcggtg
    13561 aagatctcgg cgaacagcgt ctcgtggcgc gagcggttgc cgtaatcggc gcagatcgcc
    13621 gtgatgatcg cttccttgtt ctcgcgcacg aatcgctgca gcttgcgcag gtcgccctgg
    13681 cgctcggcga gcgcgggcac gggatgcgcg aggtaggctt cgcgctgcag ggcgagcgtg
    13741 gcctggagtt cggcgttagc ggcgctgaat tgctgaagcg gctcggctcc catcgcggcg
    13801 atcctcgaca aaggtgcgtg gcacacgagt ctatcacgcg cggcattcgc cattgtgccc
    13861 ggcattggcg ctgccactac aatgccacga tgccgcgcga gaggagtgac ggagccgtga
    13921 agatcaactc ggtcgtggac tatgccgtcg aagccggtgt cgcggtgatc acggttgatt
    13981 cgccgccggt gaacgcgctg tccgcgccgg tgcgtgccgg cgtctatcgc ggcgtcgaga
    14041 ccgcgacgac cgatgcggcg gtgggcgcga tcgtgctgat ctgcgcgggc cggacgttct
    14101 gcgccggcgc cgacatcacc gagttcggca agccgccggt gtcgccattg ctgcaggacc
    14161 tgcagagcct tatcgagaac gcgcccaagc cggtggtggc ggcgatccac ggcacggcgc
    14221 tcggcggcgg actcgagctg gcgctggtgg cgcattaccg cattgccgtg cctgccgcca
    14281 gctgcgggtt gcccgaggtg aacatcggcc tgatcccggg ggccggcggt tcgcagcgcc
    14341 tgccgcgcgt tgtcggcgtg gaggcggcac tggagatgat caccagcgcc cgccacgtgc
    14401 cggcggcgca agcgcatgcg atgggcttgc tggacgtgct ggcgcagggt cccgacctgc
    14461 gtgccgcggc cgtggccttc gcgcgccggg tcatggccga gggccgcccg ctgcgcaagg
    14521 tgcgcgagct ggacgcgaaa ctgcaagccg cgcgcgccgc gccgggactg ttcgcggagt
    14581 tccgcgccgg gcacgcgcgg cagttccgcg gtttcctggc gccggagtac gcgatccgcg
    14641 ccgtcgaggc ggccgtgaac ctgcccttcg atgccgggat cgcggaggaa aagcgcctgt
    14701 ttcgcgaact gctggagggc tcgcagtccg cggcgcagcg ccatgccttc ttcgccgagc
    14761 gccgggcggc gaaggttccc ggtgttcccg ccgacatgcc gacccggacc atcgcacgcg
    14821 tgggcgtgat cggtgccggc accatgggcg gcggcatcgc gatgaatttc ctctcggccg
    14881 gcatcccggt ggtgctggtc gagacttcgc gcgaggcgct cgatcgcggc atcggcgtga
    14941 tccgccgcaa ctacgagaac tccgcgaaaa ggggcgcgtc gggcgccgcc gacgtggcgc
    15001 gcaacatggc gctgctcacg ggttcgctcg cgctcgggga tctcgctgac tgcgatctcg
    15061 tgatcgaggc ggtgttcgag cagatggcga tcaagcagga cgtgttcgcg cgcctggacc
    15121 gcgtcgccaa acccggcgcg atcctcgcca gcaacacctc ctatctcgac atcgacgcga
    15181 ttgccgccag cacctcgcgt ccgggagatg tgctgggcct gcacttcttc tcgccggcga
    15241 acatcatgcg gctgctggag atcgtgcgtg ggcgcgacac tgcagtcgat gtgatcgcca
    15301 cggcgatgaa actggccaga accatcgcca aggtcggcgt ggtggtgggc aactgccacg
    15361 gcttcgtcgg caaccgcatg ctcgcggcgc gcaaccgcga ggccgaccgg ctggtgctcg
    15421 agggtgcgct gccgtgggac gtggatcgcg tgctgtacga gttcggtttc ccgatgggcc
    15481 cgttccggat gcgcgacctg gcgggtctgg acatcggttg ggatgccgcg aagtcctctt
    15541 catcgacggt gcgcgagttg ctctgcgaga tggggcggcg cgggcagaag accggcgccg
    15601 gctattacga ccaccacgcc gcgcgcaatg ccgcgccctc ggcggtcacc gagcggctga
    15661 tcctcgattt cgccgcgcgc cagggtgtcg cgcggcgcgc gatagccgac gaggaaatcc
    15721 tcgaacgctg cctgtacccg atggtgaacg agggcgcgaa gatcctcgag gagggcatcg
    15781 cggcacgcgc ctcggacatc gacacggtgt ggctgtgcgg ctacggctgg ccgcgctacc
    15841 gcggcgggcc gatgttctgg gccgagctcg aggggctgcc gaagatcctc gggcgtctgc
    15901 gcgcgctgca gcaggtgcac ggcgacgagt tccgccccgc gccgctgctg gaacggctgg
    15961 tcgcgcaggg cgggagtttt cacgatttcg cgggccctga tgccggttga gtggtcgacc
    16021 ggcgcgcgga cgccgcgttg cgctcagcgt ggttccagcc gcgcctccat caggcgtcgc
    16081 ccgtcgttgt cgatcgcggc gagatccgtg cctccccctg tttcccttcc tgccaggtgc
    16141 agcagctggc cggcgtaggc cggcgccacc gcgcggaacg aaaaacgcgc cggatcgctc
    16201 ggattcccgc gcgttgccgc gtggccgagc agcagcgtgg cgagcagcgg cgcctgcaca
    16261 acgagggctg gatagccttc gtcgtgctgc gcgtagggca ggtcgtagtg gatgcggtgc
    16321 gcgttgaacg tgagggcgga gtagcgaaac agcagcggtg ccgaggggat ccggctgcgg
    16381 gtgaattccc agtcggagag atcggcgccg ccgctccccg gcagcgcctg catgccggcg
    16441 gcggcctcgc gccagaccag cgtctggcgt tcgcgcaccg cgggcgcgtc gtcggcaaac
    16501 gtcaggtgat cgagctcgac gaagaccgtg gcgccggatc tgctgcgctt ctcactcacc
    16561 gccgccacgc gcgagcggcg gcggatcgcg gcgccgctgc gtaacggcgc aaggaagtcc
    16621 acgctgccgg atgtccacat ccgccgcggc aatggcaccg gcggcaggaa tccgcccgtg
    16681 cgcggatggc cgtcgccgcc gagcgcgctc atcgcatcct cgggcgtgca cagacaccag
    16741 tgcacgcccc gcggcgcgat gccctcgctc tcggcgacgc ggctctcgag cgtggcgcga
    16801 aagcgtgcca gcaggccctc gccgaggagg tcgtgtcgct cctcctcgcg gccgatccag
    16861 gtgcgcagtt gctccatcac cggaccctcc tccaatcgcc ccgggtgccg ggcttcatcg
    16921 tggcgctgcc tcgcggacgg tggccagcag gtggatcgcg aggctgcgcc gctcgggctc
    16981 cgacagcggg aat
//
LOCUS       DAORUQ010000059        16894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3122_length_16894_cov_6.086882, whole genome shotgun sequence.
ACCESSION   DAORUQ010000059 DAORUQ010000000
VERSION     DAORUQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16894
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_16894_cov_6.086882"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /gene="scpA"
                     /locus_tag="PLW13_07135"
     CDS             <1..1715
                     /gene="scpA"
                     /locus_tag="PLW13_07135"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HQY70183.1"
                     /translation="FDGIPLDKVSVSMTMNGAVLPVLAGYIVAAEEQGVAQDQLSGTI
                     QNDILKEFMVRNTYIYPPAPSMRIIGDIIAYTSRNMPRFNSISISGYHIQEAGADAAL
                     ELAYTLADGKEYVRTAIAAGLEIDEFAPRLSFFFGIGMNFYMEVAKLRAARLLWARII
                     DGFGPKSAKSKMLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDE
                     AIALPTEFSARIARNTQLIIQEETGITNTIDPWGGSYLMESLTQQIADRAWELIAEVE
                     EQGGMARAIETGMPKMRIEESAARKQARIDRGEDVIVGVNKYRLATEDAVDILEIDNR
                     AVREAQLERLAVVRARRDAKAVQAALDALTQAAKTGEGNVLELAVNATRLRATVGEIS
                     SALEKEWGRYSASANTISGVYGSAYEKDEDWAALRADIEDFVERHGRRPRMLVCKMGQ
                     DGHDRGAKVVATAFADVGFDIDLSPMFSTPDEVARQAVENDVHVVGASSLAAGHKTLV
                     PELIEALRAQGAGDIVVIAGGVIPRQDYEFLYEAGVKCVFGPGTRIPLAARQVLAAIE
                     AAAG"
     gene            1727..2452
                     /gene="meaB"
                     /locus_tag="PLW13_07140"
     CDS             1727..2452
                     /gene="meaB"
                     /locus_tag="PLW13_07140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQY70184.1"
                     /translation="MIDTAALRAGNRRALAKAITLVESSRAEHRAEAQRLLEALLPHT
                     GRSIRVGISGIPGVGKSTFIEAFGMHLVAAGHRVAVLAVDPSSPVAGGSILGDKTRME
                     TLSREDGAFIRPSPSQGALGGVAHKTRETMLLCEAAGYDVIIVETVGVGQSEYEVASM
                     VDFFLVLMLPNAGDELQGIKRGIMELADALVINKADGESLGLANRTRQHYQNAMNLLS
                     HGGFWVPRVTTCSAAEKRGIAKM"
     gene            2456..2674
                     /locus_tag="PLW13_07145"
     CDS             2456..2674
                     /locus_tag="PLW13_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70185.1"
                     /translation="MIGKYRQGGEASGELAARRARQNGEWLRKLIGELLEQRFRADPR
                     VRADLPRLQARVVRGEITPYAAAMRLLG"
     gene            complement(2682..3497)
                     /locus_tag="PLW13_07150"
     CDS             complement(2682..3497)
                     /locus_tag="PLW13_07150"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HQY70186.1"
                     /translation="MDRTAFFISDGTGITAETLGEALLAQFEDVRFEFVRLPYVDTEE
                     RARHAVEQIDAAFARDGHRPIIFDTIIDQRLRQILAASAGSMFDIFTTFLAPLERAIG
                     TQSSFTVGRFHGPQSSLYHARIEAVHYAMANDDGASGSEYQKADVILIGVSRSGKTPS
                     CIYLAMQFGVHAANYPITDDDLQSNRLPVSLSAHQNRLFGLTIDPVRLAEIRHERRPG
                     SRYASLRQCEDEVRQVEGMLRRNRIPYLNTTRMSVEEIATRIMMEKGLRGRKG"
     gene            3586..5955
                     /gene="ppsA"
                     /locus_tag="PLW13_07155"
     CDS             3586..5955
                     /gene="ppsA"
                     /locus_tag="PLW13_07155"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HQY70187.1"
                     /translation="MTQQLIWFEQLGMHDVPRVGGKNASLGEMISGLAHAGVRVPDGF
                     ATTADAYREFLAHDGLAARIAARLAALDVDDVAELARAGAEIRGWIRATPLPAPLRDA
                     VGEAYARLGAGKDVAVAVRSSATAEDLPDASFAGQQETFLNISGLERVIEAVHEVFAS
                     LFNDRAISYRVHKGFDHGQVALSAGVQRMVRSETGASGVMFTLDTESGFNGTVFITAA
                     YGLGETVVQGAVNPDEFYVFKHGLREGKAAILRRNLGDKKIRMVYTAESAVGATTVTE
                     PVPEELQRRFCISDEEVTELGHIALRIEEHYGRAMDIEWARDGDDGRLYVLQARPETV
                     RSQQGAGSVERYLLKEKGRVLCEGRAIGHKIGSGTVRVIRDVSAMEQVREGDVLVTDM
                     TDPDWEPIMKRAAAVVTNRGGRTCHAAIIARELGIPAVVGCGNATESIPDGALVTVSC
                     AEGDVGHVYDGRLDYEVNADDLTAMPPLPFKLMMNVGNPDRAFDFCQLPNDGVGLARL
                     EFIINRMIGVHPKALINFDSLTGDLRQAVARRVHGYASPTDYFVDKLAEGVATIAAAF
                     HPKPVIVRMSDFKSNEYAHLVGGQQYEPVEENPMLGFRGASRYVDRGFRDCFELECRA
                     LRFVREDMGLDNVEVMVPFVRTVEEGRQVVELLAANGLARGENGLRVIMMCEIPSNAL
                     LADRFLQHFDGFSIGSNDLTQLTLGLDRDSGLVAGIFDERDEAVKTLLKLAIDACNRN
                     GRYIGICGQGPSDHPDLAAWLVEQGIQSMSLNPDVIVDTWTALAKLNQA"
     gene            complement(6177..6992)
                     /locus_tag="PLW13_07160"
     CDS             complement(6177..6992)
                     /locus_tag="PLW13_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin"
                     /protein_id="HQY70188.1"
                     /translation="MRAGDRVAGFGLALLLILSIFSTMAHAELRVLEPRHADPESLLP
                     VVQALLGPGESVSVFGNALVVNAGAGTQHRIDELLGQLDRPQRNLLISVRRGGSAVQH
                     AQGAGVEGTLQRGNVRIGPGGNTPAGSTVQIRGGRQITTRSGSGESSVRTLEGRPVLI
                     RDGALVPLAGNGYWGTGVQYEDVSQGFVVRARLAGDQVRLDIEARNDRLEGGTVRTGA
                     LASSVSGRLGEWIPLGGSTAGSESTRSGLTTRHSTRGSADELVEVRVETIDAP"
     gene            7064..7648
                     /locus_tag="PLW13_07165"
     CDS             7064..7648
                     /locus_tag="PLW13_07165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQY70189.1"
                     /translation="MAEPQRCPWVDLRKPLYVVYHDTEWGVPVHDDRLMFEYLVLEGA
                     QAGLSWYTVLSKRENYRRAFDGFDPVKMAAWDDAKIESLLRDPGIIRNRLKIQASRGN
                     ARCFLALCEERGSFSDYLWSFVGATVRAGAPATVEDYRASSPESDALSRDLKRRGFRF
                     VGSTVMYAHMQATGMVNDHSRDCFRRLQLSDAHA"
     gene            complement(7629..8372)
                     /locus_tag="PLW13_07170"
     CDS             complement(7629..8372)
                     /locus_tag="PLW13_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQY70190.1"
                     /translation="MSKSGTPKKRAAARPAARGASTRTPREEAPPRSESPLGTLFDSL
                     TDLTTDLTSSALRLASRTGASAARAIARTPEQLRMMASAGESLRDMREVTGLTVLEIS
                     DALNLRDKSVWEAVEDGREALSFELILRLASLVARNDPLPFVLKYTRTYHPRLWEILH
                     ELKIDQLPIQFERERNFINIYRRHDTARTLSDEGFERVLKFTQHAFDMAIHFVDEGEN
                     AAMEDAEAAHAGGQARGSARKRGVRRARR"
     gene            complement(8426..>9964)
                     /locus_tag="PLW13_07175"
     CDS             complement(8426..>9964)
                     /locus_tag="PLW13_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQY70191.1"
                     /translation="DGASIYDQLHYWPLQEPAQDDGELCVPASLIGLADDPYGDGHDD
                     LWSRLRAGYGLEGRVDEHRVDQYIDTYSDKQRLFDRLGSDASPFLHYVVEELEQRNMP
                     LEIALLPIIESGYNPEAVSPGKAAGLWQIVPGTASTLGLEQNGWYDGRKDVVASTEAA
                     LDYLEELHRRFDGDWYLALAAYNTGEGNVQRAIDQNRRLGKPTDYWSLPLSRQACNYV
                     PKLLALSRVLEAPQDHGISLSPMANEVSVVPVEVNKQVDLHRAATRAGMDSRELMSLN
                     PAYKRGFTAPNTSNTLLVPADDKQSFLAAVSGQAASNAPVVSAHPEHYRVRPGDNLRA
                     IARLHQTTPEALRSLNRLEDDRIAAGQELLIPADAVVPPRRFPTAESVALKNHGLYTV
                     KPGDSLSQIARRFGTSTQQLAVLNGIGTGSTLRVGQKLKVRDGLARDPAVAASATADG
                     RRRVSYTVQGGDSVARIADRFNVDVSEVLAWNGMNPARPLIHPGQVLVLYVEQKLADA
                     RKPG"
     assembly_gap    9965..10064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10065..10754)
                     /gene="gloB"
                     /locus_tag="PLW13_07180"
     CDS             complement(<10065..10754)
                     /gene="gloB"
                     /locus_tag="PLW13_07180"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HQY70192.1"
                     /translation="MLQAHPVQAFSDNYIWLLEAGAGRAFVVDPGDAGPVLLALAARG
                     LRLAGILVTHHHPDHVGGVAQLLSRCGPVPVYGPRGSPASDITHPLDDGDSIDVLGFA
                     ADVLAVPGHTLDHIAYFFAAPGAAAPLLFCGDTLFAGGCGRIFEGDPRMMHASLARLA
                     ALPAMTSVYCAHEYTLSNLRFARAVEPQNAQLLRRQQDAEALRERGEPTVPSTIALEL
                     ATNPFLRCGQAT"
     gene            10860..11630
                     /locus_tag="PLW13_07185"
     CDS             10860..11630
                     /locus_tag="PLW13_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY70193.1"
                     /translation="MTKRRFIDDPAQRWHALADWLEGDLGAHVLAAERTLLESKLPGL
                     YGFHLMQLGINDRVRLYESSMIRHKFTLGRFADARGASACAEPEHLPIESDSVDVALL
                     HHVLEYSAQPHQLLREAARVVVPHGHLLIVGFNPWSLFGLRAAATRRVAHPVWGGATL
                     TPKRVKDWLELLDFAVDDVQCGFHAIPIDHAPTLSRLARLDRAAARWSLPCGGVYLIH
                     ARKQQSPLTPVKPLLRRMRPRLSAVPLATPSVRDTTIH"
     gene            11643..12101
                     /gene="rnhA"
                     /locus_tag="PLW13_07190"
     CDS             11643..12101
                     /gene="rnhA"
                     /locus_tag="PLW13_07190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQY70194.1"
                     /translation="MKHVEIFTDGACRGNPGPGGWGALLRYAGTERELHGGEPHTTNN
                     RMELTAAIRALAALREPCRIDVYTDSQYVRSGITEWMKNWKKRGWRTADRQPVKNADL
                     WQALDEQAGRHAVQWHWVRGHSGHPENERADALANRGIDELADRLAVEGG"
     gene            12101..12826
                     /gene="dnaQ"
                     /locus_tag="PLW13_07195"
     CDS             12101..12826
                     /gene="dnaQ"
                     /locus_tag="PLW13_07195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQY70195.1"
                     /translation="MRQIVLDTETTGLEYSQGHRIIEIGCVELLDRRLTGRHFHQYIN
                     PGRPVDEGAVEVHGITTAFLADKPPFAQIVDEFLAFVSGAELVIHNAPFDLGFLDNEL
                     ALLGPGYGRIADHCTHTDTLALARARHPGQKNNLDALCKRYFVDNSQRDLHGALLDAE
                     ILADVYLHMTGGQTALALGGDSDSAAQGFSGEIQIVRLPATRARLRVLKPCAEELESH
                     RQRLAAIAKASGGKCLWTAAGEE"
     gene            12914..14596
                     /locus_tag="PLW13_07200"
     CDS             12914..14596
                     /locus_tag="PLW13_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70196.1"
                     /translation="MYFEPVLNPASLNIVRPELSRLLRQAQADFALATQPASEGQGLD
                     ACVAALQQADGVLRLLELTDAAQLARELAAVIGASPVADAVACDAVSRALHVLARYPD
                     YLAGCTHAVPQVLLEDINAMRALQSLPEFPETCFLPQCRASACQCPVLQPAGVAQVLD
                     EGLPRRLRHLYQVGLLGVIRGHTDPVHLRLMQRAAERMLGLTGGGAAGEYWWLLGGVL
                     EGFAGGALLPGRSRLRALSRADAMFRDYLRHPERRDEPVSPELREEMLGLVARAGEGM
                     RASDIRARTGMGAVALGDAQLEEERARLLGVSTEALDAMVTAVGAEFRATREALEELA
                     AHGEVRAAALDGVRDALARVQERLQAGGLRGAALLLAREMDTLDTRRADGAALGKDEL
                     LNLADAIVQTEASLGAFVRSQRGAGAQRSAAALAAPAAARDIADEAQLMLLRSARDCL
                     DAVKRDVNAYVDSRYDVQTLASSTAQLNAVRGALLMLEHPRAAAIAAGMIDALGRGSP
                     EHAAGVHERIAEVLADVLICLEYYLAALENAETPDQGMIALAQQSLAQLQAA"
     gene            14684..15748
                     /gene="sohB"
                     /locus_tag="PLW13_07205"
     CDS             14684..15748
                     /gene="sohB"
                     /locus_tag="PLW13_07205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HQY70197.1"
                     /translation="MWDFLAQYGLFLAKAVTVVIAVVFCAVGIVAAAGRARSGAGGEH
                     GHIEVRKLNDRLDMMRDALRAATLDSKEFRKLRKSDRKAEKARHKSRPTPSDTEPAPP
                     RRVYVLNFHGDIRAQAVQNLREEITALLTLAEPADRVVVRLESGGGMVHSYGLAAAQL
                     ARVRERGIPLTVCVDKVAASGGYLMACVAERVVAAPFAMLGSIGVVAQIPNFHRLLQK
                     HDIDYEMVTAGQYKRTLTVFGHNTDAAREKFTEELEDTHALFKEFVSRYRPALDMQAV
                     ATGEAWFGERALALGLVDALGTSDALLQEAARASDVLEIRYVERKGLGERLGLTIEGA
                     VERGVARAAERLDGRGNWLR"
     gene            complement(15771..15980)
                     /locus_tag="PLW13_07210"
     CDS             complement(15771..15980)
                     /locus_tag="PLW13_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70198.1"
                     /translation="MHRICTAIVLLIACYACPGAARAADNCARWEQQIQTVQQQMRRS
                     HTAAQGNRLRARLRDLQQRVAHGCR"
     gene            16079..16741
                     /gene="mobA"
                     /locus_tag="PLW13_07215"
     CDS             16079..16741
                     /gene="mobA"
                     /locus_tag="PLW13_07215"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HQY70199.1"
                     /translation="MIRPAPHAVAANAGIAGIVLAGGRGQRLGRGEKGLCRLGGRSLL
                     ERALARAAPQTGSLLLSFNGDASRLPACVVPVIPDTVPGFAGPLAGVLAGLEYLRVYQ
                     PGTRWLASFACDCPWFPHDLVQRLWHHAQHETCEVAVARSAGRLHPVFALWSCDLATP
                     LRDALVRGDMRGAGEFLRTRRHAAVDWATTSFDPFFNINTPEDLAQAEARLASGKGDD
                     CA"
BASE COUNT         2621 a         5623 c         5926 g         2624 t
ORIGIN      
        1 tgttcgacgg catcccgctc gacaaggtct cggtgtcgat gacgatgaac ggcgccgtgc
       61 tgccggtgct cgcgggctac atcgtggccg ccgaggagca gggcgtcgcg caggatcagc
      121 tctcggggac gatccagaac gacatcctca aggagttcat ggtccgcaac acctacatct
      181 acccgcccgc gccgtcgatg cggatcatcg gcgacatcat cgcctacacc tcgcgcaaca
      241 tgccgcgctt caactcgatc tcgatctccg gctaccacat ccaggaggcc ggcgccgacg
      301 ccgccctcga actcgcctac acgctcgccg acggcaagga atacgtgcgc accgcgatcg
      361 cggcgggcct ggagatcgac gagttcgcgc caaggctgtc gttcttcttc ggcatcggca
      421 tgaacttcta catggaagtg gccaagctgc gcgccgcccg gttgttgtgg gcgcggatca
      481 tcgacggctt cggcccgaag agcgcgaaat cgaagatgtt gcgcacgcac tgccagacct
      541 cgggctggtc gctcaccgag caggatccgt acaacaacgt ggtgcgcacc acgatcgagg
      601 ccatggcggc ggtcttcggc ggcacgcagt cgctgcacac caatgcactc gacgaggcca
      661 tcgcgttgcc gaccgagttc tcggctcgca tcgcgcgcaa cacgcaactg atcatccagg
      721 aggagaccgg catcaccaac acgatcgatc cgtggggcgg ttcctacctg atggaatcgc
      781 tgacgcagca gatcgccgac cgcgcctggg agctcattgc cgaggtcgag gagcagggcg
      841 gcatggcccg cgccatcgag accgggatgc cgaagatgcg catcgaggag tcggccgcgc
      901 gcaagcaggc gcggatcgac cgcggcgagg acgtgatcgt cggcgtcaac aagtaccgcc
      961 tcgccaccga ggatgccgtg gatatcctcg agatcgacaa tcgcgccgtg cgcgaggcgc
     1021 agctcgagcg cctcgccgtg gtccgcgcgc gccgtgacgc gaaggccgtg caggcggcgc
     1081 tggatgcgct cacgcaggca gcgaagaccg gcgagggcaa cgtgctcgag cttgccgtaa
     1141 atgccacgcg cctgcgtgcc accgtcggcg agatctcctc ggcgctggag aaggaatggg
     1201 gccgctacag cgcgagcgcg aacacgatct ccggcgtcta cggcagtgcc tacgagaagg
     1261 acgaggactg ggccgcgctg cgcgccgaca tcgaggactt cgtcgagcgc cacggccgcc
     1321 gcccgcgcat gctggtgtgc aagatgggtc aggacggtca cgatcgtggc gccaaggtgg
     1381 tggcgacggc cttcgccgac gtcggcttcg atatcgacct ctcgccgatg ttctccacgc
     1441 cggacgaggt cgcgcgccag gccgtcgaga acgacgtgca cgtggtgggc gcctcgtcgc
     1501 tggccgcggg ccacaagacc ctggtgcccg agctgatcga ggcgctgcgc gcgcagggcg
     1561 cgggcgacat cgtggtgatc gccggcggcg tgatcccgcg ccaggactac gagttcctgt
     1621 acgaggccgg ggtcaagtgc gtcttcgggc ccggcacgcg catcccgctg gcggcgcgcc
     1681 aggtgctcgc cgcgatcgag gcggcggcag gctgaggcac gagcccgtga tcgacaccgc
     1741 ggcactgcgc gcgggcaacc gtcgcgcgct ggccaaggcc atcacgctgg tggagagttc
     1801 gcgcgccgag catcgcgccg aggcgcagcg gctgctggag gcgttgctgc cgcacacggg
     1861 taggagcatc cgcgtcggca tcagcggcat cccgggcgtg ggcaagtcga cgttcatcga
     1921 ggccttcggc atgcacctgg tcgccgcggg ccaccgcgtg gcggtactgg ccgtggatcc
     1981 gagttcgccc gtggccggtg gcagcattct cggcgacaag acgcgcatgg agaccctctc
     2041 gcgcgaggac ggggcgttca tccggccctc gccgtcgcag ggtgcgctgg gcggcgtggc
     2101 gcacaagacg cgcgagacca tgctgctctg cgaggccgcg ggctacgacg tgatcatcgt
     2161 cgagaccgtc ggggtggggc aatccgagta cgaggtcgcc agcatggtcg acttcttcct
     2221 ggtgctgatg ctgcccaacg ccggcgacga actgcagggc atcaagcgcg gcatcatgga
     2281 actggccgac gcgctggtga tcaacaaggc cgacggcgag agcctgggac tcgccaaccg
     2341 cacccgccag cattaccaga acgcgatgaa cctgctatcg cacggcggct tctgggtgcc
     2401 gcgcgtcacg acctgctcgg cggccgagaa gcgcggcatc gccaaaatgt agcagatgat
     2461 cgggaaatac cgccagggcg gggaggcgag cggcgagctc gcggcgcggc gcgcgcggca
     2521 gaacggcgag tggctgcgca agctgatcgg cgagctgctg gagcagcgct ttcgtgccga
     2581 tccgcgcgtg cgcgccgatc tgccgcggct gcaagcgcgc gtggtgcgcg gcgagatcac
     2641 cccctacgcc gcggcgatgc gcctgctcgg ctgacgaatc ctcatccctt gcggccgcgc
     2701 aggccctttt ccatcatgat ccgcgtcgcg atttcctcca ccgacatgcg cgtggtgttc
     2761 aggtacggga tgcggttgcg tcgcagcatc ccctcgacct ggcgcacctc gtcctcgcat
     2821 tgcctgagcg aggcatagcg gctgccgggg cggcgttcgt ggcggatctc ggccaggcgc
     2881 accgggtcga tcgtgagtcc gaacaggcgg ttctggtggg cgctgagtga taccggcagc
     2941 cggttcgact gcaggtcgtc gtcggtgatg gggtaattgg ccgcatgcac gccgaactgc
     3001 atcgccaggt agatgcacga gggcgtcttg ccgctgcgcg agacgccgat caggatcacg
     3061 tcggccttct ggtactcgct gccgctggcg ccatcgtcgt tggccatcgc gtagtgcacg
     3121 gcctcgatgc gtgcgtggta caggctgctc tgcggcccgt ggaagcgccc gaccgtgaag
     3181 ctcgactggg tcccgatggc gcgctccagc ggtgcgagga aggtcgtgaa gatgtcgaac
     3241 atgctgccgg cggacgcggc gagaatctgg cgcaggcgct ggtcgatgat cgtgtcgaag
     3301 atgatcgggc ggtggccgtc gcgcgcgaag gcagcgtcga tctgctccac ggcgtggcgg
     3361 gcgcgttcct cggtatcgac atagggcagg cgcacgaact cgaagcgcac gtcctcgaac
     3421 tgcgcgagca gcgcctcgcc gagcgtctcg gccgtgatgc cggtgccgtc ggagatgaag
     3481 aatgcggttc ggtccatggc tgttttcccg ctgtctggcg ggcggatttt gtcacagcgc
     3541 tcaggtaaca tgtaggccgc ttcgagaacc gcgggagtat tcaaggtgac gcagcaactg
     3601 atctggttcg aacagctcgg catgcacgac gtgccgcgcg tcggtggcaa gaacgcctcg
     3661 ctcggcgaga tgatcagcgg cctcgcgcac gccggcgtgc gcgtgcccga cggtttcgcc
     3721 accaccgccg acgcgtatcg cgagttcctg gcgcacgacg ggctggcggc gcgcatcgcg
     3781 gcgcgtctcg cggccctgga tgtcgacgac gtcgcggagc tggcgcgcgc gggagcggag
     3841 atccgcggct ggatccgcgc gacaccgctg cccgcgccac tgcgcgacgc ggtgggcgaa
     3901 gcctatgcac gcctcggcgc gggcaaggat gtcgcggtcg ccgtgcgctc ctccgcgacc
     3961 gccgaggatc tgcccgatgc ctccttcgcc gggcagcagg agaccttcct caacatcagc
     4021 ggcctcgagc gcgtgatcga ggcggttcac gaggtgttcg cctcgctgtt caacgatcgg
     4081 gcgatctcct atcgcgtgca caagggcttc gaccatggcc aggtcgcgct gtcggccggg
     4141 gtgcagcgca tggtgcgcag cgagaccggc gcgagcggcg tgatgttcac gctggacacc
     4201 gagtcgggct tcaatggcac cgtgttcatc accgcggcct acggtctcgg cgagaccgtg
     4261 gtgcagggcg ccgtcaaccc ggacgagttc tacgtcttca agcacggcct gcgcgagggc
     4321 aaggcggcga tcctgcggcg taacctcggc gacaagaaga tccgcatggt ctacaccgcc
     4381 gaatccgcgg tcggtgcgac cacggtgacc gagccggtgc ccgaggagct gcagcgacgg
     4441 ttctgcattt ccgacgagga ggtgactgag ctcggtcata tcgcgctgcg catcgaggag
     4501 cactatggcc gtgcgatgga catcgagtgg gcgcgcgacg gcgacgacgg caggctctac
     4561 gtgctgcagg cgcggcccga gaccgtgcgc agccagcagg gcgcgggcag cgtcgaacgt
     4621 tacctgttga aggaaaaggg ccgggtgctc tgcgagggcc gggcgatcgg gcacaagatc
     4681 ggctcgggca cggtgcgcgt catccgcgac gtctcggcga tggagcaggt gcgcgaaggc
     4741 gacgtgctgg tcaccgacat gaccgacccc gactgggagc cgatcatgaa gcgcgcggcc
     4801 gcggtggtga ccaaccgcgg cgggcgcacc tgccacgcgg cgatcatcgc gcgcgaactg
     4861 ggtattccgg cggtcgtcgg ttgcggcaac gcgacggagt cgatccccga cggcgcgctg
     4921 gtcacggtct cctgcgccga gggtgacgtg ggacacgtct acgacggcag gctcgactac
     4981 gaggtgaacg ccgacgacct caccgcgatg ccgccgctgc cgttcaagct gatgatgaac
     5041 gtgggcaatc cggaccgggc gttcgacttc tgccagttgc cgaacgacgg cgtggggctg
     5101 gcgcgcctgg aattcatcat caaccgcatg atcggcgtgc acccgaaggc gttgatcaac
     5161 ttcgattcgc tgacgggcga cctgcgccag gccgtggcgc ggcgcgtgca cggctacgcc
     5221 tcgcctaccg attacttcgt cgacaagctg gccgagggcg tggcgacgat cgccgcggcc
     5281 ttccatccga agccggtgat cgtgcgcatg tcggatttca agtccaacga gtacgcgcac
     5341 ctggtcggcg gccagcagta cgagcccgtc gaggagaatc ccatgctcgg ctttcgcggt
     5401 gcctcgcgct acgtcgatcg cggtttccgc gactgcttcg agctcgaatg ccgcgcgctg
     5461 cgcttcgtgc gcgaggacat gggcctggac aacgtcgagg tcatggtgcc tttcgtgcgc
     5521 acggtggagg aggggcgcca ggtggtggaa ctgctcgccg cgaacgggct cgcgcgcggc
     5581 gagaacgggc tgcgcgtgat catgatgtgc gagatcccga gcaacgcgct gctggcggac
     5641 aggttcctgc agcatttcga cggcttctcg atcggctcta acgacctcac gcagctcacg
     5701 ctgggtctcg atcgcgattc gggcctggtg gccggcatct tcgacgagcg cgacgaggcg
     5761 gtcaaaacgc tgctgaagct ggcgatagac gcctgcaacc gcaacgggcg ctacatcggc
     5821 atctgcggcc agggtccctc ggaccacccg gacctggcgg cctggctggt ggagcagggg
     5881 atacagagca tgtccctgaa ccccgacgtc atcgtcgata cctggaccgc gctggcgaag
     5941 ctgaaccagg cctaaccgtg gcgtgggtct catgtgcccg atgcggcacg ggctcaaccg
     6001 agtggctgtg ctgcccgctg ttggcttgcg gggccttgcg ccgcaggacg cggcgcggag
     6061 ctacagggac gtactcgtgc gtgccccgca ggacaatggc gggcagcacc gccagcgacg
     6121 caccaggctg ctcgctcgca acctgatggt ttttgcgaca ccctctgaag ccgcagctac
     6181 ggggcgtcga ttgtctccac gcgcacctcg accagttcat cggcgctgcc gcgcgtgctg
     6241 tggcgcgtgg tgaggccgct gcgggtgctc tcgctgccgg cggtggagcc gccgagcggg
     6301 atccattctc cgaggcgtcc gctcacgctg ctggcgagcg cgccggtgcg cacggtgccg
     6361 ccctcgaggc ggtcgttgcg cgcctcgata tcaagccgca cctgatcgcc cgcgaggcgc
     6421 gcgcgcacca cgaagccctg gctcacgtcc tcgtactgca cgccggtgcc ccagtagccg
     6481 ttacccgcga gcgggacaag ggcgccgtca cgaatcagca cggggcgccc ctcgagggtg
     6541 cgcacgctgc tttcgccgct gccgcttcgc gtcgtgatct gcctgccgcc gcgaatctgc
     6601 accgtgctgc cggctggcgt gttgcccccc gggccgatgc gaacattgcc acgctgcagc
     6661 gttccttcca cgcccgcacc ctgcgcatgc tgcacggccg aacccccgcg gcgcacgctg
     6721 atcagcaggt tgcgctgcgg gcggtcgagc tgcccgagca gttcgtcgat cctgtgctgg
     6781 gtcccggcac ctgcattcac caccagcgcg ttgccaaaga cactgaccga ctccccgggg
     6841 cccagcagcg cttgcaccac cggcagcagc gattcggggt cggcgtgacg aggctccagc
     6901 acgcgcaatt cggcgtgagc catggtcgag aagatggaca ggatcagcag gagcgccagc
     6961 ccgaatccgg ctacgcggtc ccccgcgcgc atccgggagg ccgattggag cgcggctgcg
     7021 acgctgctac agtgcatcgc gcactcatcg cgggaggaag gaaatggccg agccgcaacg
     7081 ctgtccatgg gtcgatctgc gcaagccctt gtatgttgtt tatcatgaca cggagtgggg
     7141 ggtgccggtc cacgacgacc gcctgatgtt cgaatacctg gtgctggaag gcgctcaggc
     7201 gggcctcagc tggtacacag tactcagcaa gcgagagaac taccggcgcg ccttcgacgg
     7261 tttcgatccg gtgaagatgg cggcctggga cgatgcgaag atcgagtccc tgctgcgcga
     7321 tcccggcatc atccgcaacc gcctgaaaat ccaggcctcc cgtggcaatg cgcgctgctt
     7381 ccttgcgctg tgcgaggaac gcggttcatt cagcgactac ctgtggagtt ttgtcggcgc
     7441 caccgtgcgt gccggtgcgc cggcgaccgt ggaggactat cgcgcgagca gtcccgaatc
     7501 agatgcgctc agtcgcgacc tgaagcggcg cggtttccgc ttcgtgggat cgacggtgat
     7561 gtatgcgcac atgcaggcaa ccggcatggt aaacgaccat tcccgcgact gcttccggcg
     7621 cctgcaactc agcgacgcgc acgcctgacg ccgcgcttgc gcgcgcttcc ccgcgcctgt
     7681 cccccggcat gcgcggcctc ggcatcctcc attgcggcgt tttccccttc gtcgacgaaa
     7741 tggatcgcca tgtcgaaggc gtgctgcgtg aacttgagca cgcgctcgaa gccctcgtcg
     7801 gagagcgtgc gcgcggtgtc gtggcggcga tagatgttga tgaagttgcg ctcgcgctcg
     7861 aactggatcg gcagctggtc gatcttcagt tcgtgcagga tctcccacaa ccgcgggtga
     7921 taggtccgcg tgtacttgag cacgaagggc agcggatcgt tgcgcgccac cagcgaggcg
     7981 agccgcagga tcagctcgaa ggacagcgcc tcgcgtccgt cctccacggc ttcccacacg
     8041 ctcttgtcac gcaggttcag cgcatcggag atctccagca cggtcaaccc ggtgacctcg
     8101 cgcatgtcgc gcagcgattc ccctgccgac gccatcatgc gcagttgctc gggcgttcgc
     8161 gcgatcgcgc gcgccgcact cgcgcccgtg cgcgacgcga gcctcagcgc ggagctggtg
     8221 aggtcggtgg tgaggtcggt gaggctgtcg aacagcgtgc ccaatggcga ttcgctgcgc
     8281 ggcggagcct cctcgcgcgg agtgcgcgtc gaggccccgc gcgccgcggg acgagcggca
     8341 gcgcgcttct tcggagttcc gctcttgctc atccggcttc ccccggtcgc ttggaccttg
     8401 cccgcggcgt gggcgcgcgg gcgtatcagc ccggtttgcg cgcgtctgcc agtttctgct
     8461 ccacgtagag taccagtacc tggccggggt ggatcaaggg gcgggcggga ttcatgccgt
     8521 tccacgccag cacctcgctc acgtccacgt tgaatcgatc cgcgatgcgt gcgaccgaat
     8581 cgccgccctg cacggtataa ctcaccctgc ggcgaccgtc cgcggtggca ctggcagcga
     8641 cagcgggatc ccgcgccagg ccgtcgcgga ccttgagctt ctgcccgacg cgcagcgtgg
     8701 aaccggtgcc gatgccattc agcacggcga gctgctgcgt gctggtcccg aaacgcctgg
     8761 cgatctgcga caggctgtcg ccgggcttga cggtgtagag accgtggttc ttcaacgcca
     8821 cgctctcggc ggtggggaag cggcgcggcg gcactaccgc gtcggccggg atcagcaact
     8881 cctgacccgc cgcgatgcgg tcgtcctcga ggcggttcag gctgcgcagc gcctccgggg
     8941 tggtctggtg cagccgcgct atggcgcgca ggttgtcgcc tgggcgcacg cggtagtgct
     9001 cggggtgtgc gctcacgacg ggcgcgttgc tcgccgcctg tcccgatacc gcggccagga
     9061 agctctgttt atcgtccgcc gggaccagca gcgtgttgct ggtgttgggc gcggtgaagc
     9121 cgcgcttgta ggccgggttc aggctcatca gttcgcggct gtccatgccc gcgcgcgtcg
     9181 cggcacgatg caggtcgacc tgcttgttga cctcgacggg gaccaccgac acctcgttcg
     9241 ccatcgggga cagggagatg ccgtggtcct ggggtgcctc cagcacccgc gacagcgcaa
     9301 gcagcttcgg cacatagttg caagcctggc gcgacaacgg cagggaccag taatcggtcg
     9361 gcttgccgag gcgcctgttc tggtcgatgg cgcgctgcac gttgccttca cccgtgttgt
     9421 aggccgccag cgcgaggtac cagtcgccgt cgaaacgccg gtgcaattcc tcgagatagt
     9481 cgagtgccgc ttcggtcgag gcgaccacgt ccttgcgccc gtcgtaccag ccgttctgct
     9541 ccagccccag ggtcgaggcc gtgccgggga cgatctgcca caggcccgcc gccttgcccg
     9601 gactgacggc ctcggggttg tagccgctct cgattatcgg cagcagcgcg atctcgagcg
     9661 gcatgttgcg ctgttcgagt tcctctacca cgtagtgcag gaagggcgag gcatcggagc
     9721 cgagccggtc gaacaggcgc tgcttgtccg aataggtatc gatgtactgg tcgacgcgat
     9781 gttcatcgac gcgtccctcg aggccataac cggcccgcag gcgcgaccac aggtcatcgt
     9841 gcccgtcgcc gtaggggtca tctgcgagcc cgatcagcga tgccggcacg cagagctcgc
     9901 catcgtcctg cgccggctcc tgcaggggcc agtaatgcag ttggtcgtag atgctggcgc
     9961 cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgttgc ctggccgcag
    10081 cgcaggaagg gattggtcgc cagttccagc gcgatggtcg agggcacggt cggctcgcca
    10141 cgctcgcgca gggcctcggc gtcctgctgg cgacgcagca attgcgcgtt ctgcggttcc
    10201 accgcgcgcg caaagcgcag gttcgacagc gtgtattcgt gcgcgcagta aaccgacgtc
    10261 atcgccggca gtgccgcgag acgcgcgagc gaggcatgca tcatgcgcgg atcgccttcg
    10321 aaaatgcgtc cgcagccgcc ggcgaacagc gtatcgccgc agaacagcag cggagccgcg
    10381 gcgcccggcg ccgcgaagaa ataggcgatg tgatccagcg tgtgacccgg caccgcgagc
    10441 acatcggccg caaaacccag cacgtcgatg ctgtcaccgt cgtccagcgg gtgggtgatg
    10501 tcgctggccg gggagccccg cggaccatat accggcacgg ggccgcagcg gctcagcagt
    10561 tgcgcgacgc cgccgacgtg gtccggatga tggtgggtga cgaggatgcc cgcgagcctc
    10621 aggcctcgcg ccgcgagcgc gagcagcacg ggcccggcat cgccgggatc gaccacgaag
    10681 gcccgtcctg cgcctgcctc gagtagccag atgtagttgt cgctgaatgc ctgtaccgga
    10741 tgcgcctgga gcattgtcgg tgagtgaatc cgtggccggg ccgtgcatgg tatattgccc
    10801 ggacgagata gccaacccgc gagcggccca tgccgcccgc ggccaagcga ttgatcagga
    10861 tgacaaaacg acgattcatc gacgatccgg cacagcgctg gcatgcgctc gccgactggc
    10921 tcgagggcga cctcggcgcc cacgtgctcg ccgccgagcg gaccctgctg gagagcaagc
    10981 tgcccggact ttacggcttc cacctgatgc agctgggcat caacgaccgt gtgcgtctct
    11041 acgagagcag catgatccgc cacaagttca cgctcggacg cttcgcggat gcccgtggcg
    11101 ccagcgcctg tgccgagccg gaacacctgc cgatcgagtc cgacagcgtc gatgtggcgc
    11161 tgctgcacca cgtgctggaa tacagcgcgc aaccgcacca gttgctgcgc gaagcggcgc
    11221 gcgtggtggt gccgcatggt cacctgctga tcgtgggctt caatccgtgg agcctgttcg
    11281 gcctgcgcgc cgccgcgaca cgacgcgtcg cccatccggt gtggggtgga gcgacgctga
    11341 cgcccaagcg ggtgaaggac tggctggagc tgctagattt cgcggtcgac gacgtgcagt
    11401 gcggcttcca cgccatcccg atcgatcacg cgccaacgct gtcgcgcctc gcccggctcg
    11461 accgcgccgc ggcacgctgg tcgctgccct gcggcggggt ctacctcatc cacgcgcgca
    11521 agcagcagag cccgctgacg cccgtgaagc ccttgctgcg ccgcatgcgt ccgcgcctta
    11581 gcgcggtgcc gctcgcgacc ccgagcgtgc gcgacaccac gatccactga ccccggagcc
    11641 gaatgaaaca cgtagagata ttcaccgacg gggcctgccg cggcaatccc ggccccggcg
    11701 gctggggcgc gctgctgcgc tacgccggca cggagcgcga gctgcacggc ggcgagccgc
    11761 atacgacgaa caaccgcatg gagctcaccg ccgcgatccg cgcgctggcg gcgctgcgcg
    11821 agccctgccg catcgatgtg tacaccgact cgcagtacgt gcgcagcggc atcaccgagt
    11881 ggatgaagaa ttggaagaag cgcggctggc gcaccgcgga tcgccagccc gtgaagaacg
    11941 ccgacctgtg gcaggcgctc gacgagcagg ccgggcgcca cgcggtgcaa tggcactggg
    12001 tgcgcggaca cagcggccat cccgagaacg agcgcgccga cgcgctggcg aaccgcggca
    12061 tcgacgagct cgccgatcgc ctcgcggtgg agggcggctg atgcgacaga tcgtgctgga
    12121 caccgaaacc accggcctcg agtacagcca gggccaccgc atcatcgaga tcggctgcgt
    12181 ggaactgctc gatcggcgcc tgacggggcg gcatttccac cagtacatca accccggccg
    12241 cccggtcgac gaaggcgccg tcgaggtgca cggcatcacc accgcgttcc tcgccgacaa
    12301 gcccccgttc gcgcagatcg tcgacgagtt cctggcgttc gtcagcggcg ccgagctggt
    12361 gatccacaac gcgccgttcg atctcggctt tctcgacaac gagctcgcat tgctgggccc
    12421 tggctacggc cgcatcgcgg accactgcac gcataccgac acgctggcgc tcgcgcgagc
    12481 ccgccatccg ggacagaaga acaacctcga cgcgctgtgc aagcgctact tcgtcgacaa
    12541 ctcgcagcgc gacctgcacg gcgcgctgct cgacgcggag atcctcgccg acgtctacct
    12601 gcacatgacc ggcgggcaga ccgcgctcgc gctgggtggc gacagcgaca gcgcggcgca
    12661 gggtttctcc ggggagatcc agatcgtgcg cctgcccgcg acgcgggcgc gactgcgcgt
    12721 gctgaagccc tgcgcggagg aactcgagag ccatcgccag cgcctcgccg ccatcgcgaa
    12781 ggcgagcggc ggcaagtgcc tgtggaccgc cgcaggcgag gaatagcgcg cacgatcgcc
    12841 gcgaatccag cgtctatact gagccggtgc cgttggcgca gccgtgcgcg cagaattcct
    12901 gagaggtgac cgggtgtact tcgagccagt attgaatcct gcctcgctca atatcgtgcg
    12961 accggagctg tcgcggctgt tgcgccaggc gcaggccgat ttcgcgctcg cgacgcagcc
    13021 ggcaagcgag gggcagggcc tcgatgcctg cgtcgcagca ctgcagcagg ccgacggcgt
    13081 gctgcggctg ctggaactga ccgacgccgc gcaactggcg cgcgagctcg cggccgtgat
    13141 cggcgcatcg cctgtggccg atgccgtggc gtgcgatgcg gtctcgcggg cgctgcacgt
    13201 gctggcgcgt taccccgatt acctggcggg ctgcacgcac gcggtgccgc aagtgctgct
    13261 cgaagacatc aacgcgatgc gcgcgctgca gtcgctgccc gaatttcccg agacgtgttt
    13321 cctgccgcaa tgccgggcat cggcatgcca atgcccggtg ctgcagccgg ccggcgtagc
    13381 gcaggtgctc gacgagggct tgccgcggcg cctgcgccat ctctaccagg tcggcctgct
    13441 cggcgtgatc cgcgggcaca ccgatccggt ccacctgcgg ctgatgcagc gtgccgccga
    13501 gcgcatgctc ggcctcaccg gcggcggggc cgccggcgaa tactggtggt tgctgggcgg
    13561 cgtgctcgag ggttttgccg gaggcgcgct gctgccgggg cgttcgcgcc tgcgggcgct
    13621 aagtcgcgcc gacgcgatgt tccgtgacta cctgcggcat cccgagcggc gcgatgaacc
    13681 ggtgtcgccg gagctgcgcg aagagatgct ggggctggtg gcgcgcgccg gagagggcat
    13741 gcgcgcaagc gacatccgcg cacgcaccgg catgggcgcg gtggcgctcg gtgacgcgca
    13801 gctcgaggag gaacgcgcgc ggctgctcgg ggtgtcgacc gaggcgctgg atgcgatggt
    13861 caccgcggtc ggggccgagt tccgtgccac gcgcgaggca ctcgaggagc tcgccgccca
    13921 cggcgaggtc cgtgccgcgg cgctcgacgg tgtgcgcgat gccctggctc gcgtgcagga
    13981 gcgcctgcag gccggcgggc tgcgcggtgc ggcgctgctg ctggctcgcg agatggatac
    14041 gctggacacc cggcgcgccg atggcgcagc gctcggcaag gatgaactgc tgaacctggc
    14101 cgatgccatc gtgcagaccg aggcctcgct gggggcattc gtacgcagcc agcgcggtgc
    14161 gggtgcgcaa cgcagcgctg ccgcgctcgc ggcgccggcc gcggcgcgcg atatcgccga
    14221 cgaggcgcag ctgatgctgc tgcgcagtgc gcgtgactgc ctcgacgccg tcaagcgcga
    14281 cgtgaacgca tacgtggaca gccgctacga cgtgcagacg cttgccagct cgacggcaca
    14341 gctcaacgcg gtgcgtggtg cgctgctgat gctggagcac ccgcgggccg cggctatcgc
    14401 tgccggcatg atcgacgccc ttggccgcgg cagcccagag catgcagccg gtgtgcacga
    14461 gagaatcgcg gaagtgctcg ccgacgtgct gatctgcctc gagtactacc tggcggcgct
    14521 ggagaacgcc gagacccccg atcagggaat gatcgcactg gcgcagcaga gcctcgcaca
    14581 gctgcaggcg gcctgattcg cgcagagcag agatgccgcc ggcgatcgcg cgccggcaag
    14641 tatggtaggc tcgcgctccc cccggcgacc ggagactcag tgtgtgtggg attttctagc
    14701 gcaatacgga ttgttcctcg ccaaggccgt caccgtggtg atcgcggtcg tgttctgcgc
    14761 ggtcggcatc gtagcggctg cgggccgcgc acgcagcggc gccggcggcg aacacggcca
    14821 catcgaagtg cgcaaactga acgaccggct ggacatgatg cgcgatgcgt tgcgcgccgc
    14881 gacgctggac agcaaggagt tcaggaaact gcgcaagtcc gaccgcaagg cggagaaggc
    14941 gcgtcacaag tcccggccca cgccgtccga cacggaaccg gcgccaccac ggcgcgtcta
    15001 tgtgctcaat ttccacggtg acatccgcgc acaggccgtg cagaacctgc gcgaagagat
    15061 cacggcgctg ctcacgctcg ccgagcccgc ggaccgtgtg gtcgtgcggc tcgagagcgg
    15121 cgggggcatg gtgcactcct acgggctcgc ggccgcgcag ctggcgcgcg tgcgcgagcg
    15181 cggcattccg ctcaccgtgt gcgtcgacaa ggttgccgcg agcggcggct acctgatggc
    15241 ctgcgtggcc gagcgcgtgg tcgcggcacc gttcgcgatg ctcggcagca tcggggtggt
    15301 cgcgcagatc ccgaatttcc atcgcctgct gcagaagcac gacatcgatt acgagatggt
    15361 caccgcgggg cagtacaagc gcaccctgac ggtgttcggc cacaacaccg acgcggcccg
    15421 ggagaagttc accgaggagc tcgaggacac gcacgcgctg ttcaaggaat tcgtctcgcg
    15481 ataccgcccc gcgctcgaca tgcaggcggt cgcgacgggc gaggcctggt tcggcgagcg
    15541 tgcgctggcg ctcgggctcg tcgatgcact gggcaccagc gatgcattgc tgcaggaggc
    15601 ggcgcgcgcc tccgatgtgc tcgagatccg ctacgtcgaa cgcaagggac tgggcgagcg
    15661 cctggggctc acgatcgagg gcgcggtgga gcgcggcgtc gccagggcgg cagagcggct
    15721 cgacgggcgc ggcaactggc tgcgctgagc cccggagcgg tccggcgtcc tcagcggcaa
    15781 ccgtgcgcca cccgctgctg cagatcgcgc agccgcgcac gcaggcgatt gccctgcgcg
    15841 gcggtatgcg agcgccgcat ctgctgctgc acggtctgga tctgctgctc ccagcgcgcg
    15901 caattgtccg ccgcgcgcgc ggcgcccggg caggcgtaac aggcgatgag caggacgatg
    15961 gcggtgcata tccgatgcat gtgacttccc tccctggaaa ccaagtgtcc atcagacgcg
    16021 cgcgcggtgg cgctgtcaag caccgcgcgc gatcgtgcag gcacgagatg acgcagccat
    16081 gatcaggccg gcaccgcacg ccgtggcggc caacgccggc attgccggca tcgtgctggc
    16141 gggcggccgc gggcagcgcc tgggccgggg cgagaaaggt ctgtgccggc tcggcgggcg
    16201 cagcctgctg gagcgtgcac tcgcgcgtgc cgcgccgcag accggctcgc tgctgctcag
    16261 cttcaatggc gatgcgagcc gattgccggc atgcgtggtg cccgtgatcc cggacaccgt
    16321 gccgggcttc gccggcccgc tggccggcgt gctcgcgggg ctggaatacc tgcgtgtgta
    16381 ccagcccggc acgcgctggc tcgcgagctt cgcctgcgat tgcccctggt tcccccacga
    16441 cctggtgcag cgcctgtggc atcacgcgca gcacgagacg tgcgaagtcg ccgtcgcgcg
    16501 ttcggccgga cgcctgcacc cggtgttcgc gctgtggtcc tgcgatctcg ccacgcccct
    16561 gcgcgatgcg ctcgtgcgcg gcgacatgcg cggtgcgggc gaattcctgc gtacgcgacg
    16621 ccacgccgcg gtcgattggg cgacaacgtc cttcgatccg tttttcaaca tcaacacgcc
    16681 cgaggatctc gcgcaggccg aggcacggct ggcgagcggg aagggggatg attgcgcatg
    16741 aacgtgccta ccgacacctg ccaggtcacg ggcctcttcg tatacccggt gaaggcgttg
    16801 cgcggcgtcg cactggaacg atccggcatc ggtgcgcgcg gcctcgacgg cgatcgcgag
    16861 tggatgatcg tgcgcgagga cggacgtttc gtgt
//
LOCUS       DAORUQ010000060        16020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3387_length_16020_cov_6.131412, whole genome shotgun sequence.
ACCESSION   DAORUQ010000060 DAORUQ010000000
VERSION     DAORUQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_16020_cov_6.131412"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..2028)
                     /gene="typA"
                     /locus_tag="PLW13_07220"
     CDS             complement(205..2028)
                     /gene="typA"
                     /locus_tag="PLW13_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQY70200.1"
                     /translation="MREHLRNIAIIAHVDHGKTTLVDKLFQQCGTLGRRDAGKERIMD
                     SNDQERERGITILAKNTAIDWKGYRINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTRKAFEAGLNPIVVVNKVDRPGARPHWVMDQVFDLFDRLGATDEQLDF
                     PVIYASALNGVAGPEPDQLAEDMSPLLEMIVARVPPPPADSNGPFQMQICALDYSSYV
                     GVIGIGRVKRGVARPGMQVVVIEADGTRRNARINSVLGQMGMEKAEIAEGEAGDIVSI
                     TGIDNLKISSTLCDPAAPEALPVLTIDQPTVSMTFQVNDSPFAGREGQFVTSRNIRDR
                     LERELLSDVALRVQDGSTPDRFVVSGRGELHLSVLIEKMRREGFEMGVSRPEVIRRVI
                     DGVEQEPYESLLIDCEETHQGAIMEEMGKRRGELRDMVPDGKGRVRLEYMIPSRGIIG
                     FRNQFLTMTSGTGVLNSIFDHYGPVKAGEVAARQNGVLVSMVAGKVLGYALFSLQERG
                     RLFIGPNVEVYEGQLIGIHSRDNDLAVNPTKGKQLTNVRAAGSDENILLTPPIRMSLE
                     QALDFIDEDELVEVTPQSVRLRKKLLKEVERRRAGRREASE"
     gene            2393..3799
                     /gene="glnA"
                     /locus_tag="PLW13_07225"
     CDS             2393..3799
                     /gene="glnA"
                     /locus_tag="PLW13_07225"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HQY70201.1"
                     /translation="MSEQTLKLIKENDVKWVDLRFTDTKGKEQHLTIPSSEVSADFFE
                     DGKMFDGSSIAGWKAINASDMLLMPDDKSSVLDPFFEESAVILRCNVLEPDTKQGYNR
                     DPRSIAARAEEYMRSTGIADSALFGPEPEFFVFDDVKWHADISGAMYQIFSEEAAWAS
                     KHSFDDGNIGHRPGVKGGYFPVPPVDSLQDLRAAMCRAMEKMGLTIEVHHHEVGTAGQ
                     CEIGVGPNAIVQKADEVQILKYAVHNVAHAYGKTATFMPKPLVGDNGSGMHVHMSISK
                     GGKNLMAGDGYAGLSETAIYMIGGIIKHARAINAFTNPGTNSYKRLVPGYEAPVMLAY
                     SAKNRSASIRIPAVANPKARRIEVRFPDPSANPYLGFAALLMAGLDGIQNKIHPGDAL
                     DKNLYDLPAEEAAQVPTVSHSLEMALAALNADRAFLTAGGVFDDDMIDAYIELKQEDI
                     DRIRMSTHPVEFDMYYSC"
     gene            3936..4496
                     /locus_tag="PLW13_07230"
     CDS             3936..4496
                     /locus_tag="PLW13_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY70202.1"
                     /translation="MTTRTRCARALVIACLAATVAATALAQGQVYKSRDAQGNVIFSD
                     TSSERASPVTVPPTNTIPVVAAPERPVAPPATADTTAAAGYALAIVTPVEGQTVFSAV
                     GALDVSLSVSPALQPGHSLRLLLDGAPAGLPQQGSLTLEGLGPGPHTLQAQVADSNGE
                     IVQSSGTVTVQMQRSQGRRIGPVRIP"
     gene            4712..5776
                     /gene="glnL"
                     /locus_tag="PLW13_07235"
     CDS             4712..5776
                     /gene="glnL"
                     /locus_tag="PLW13_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HQY70203.1"
                     /translation="MRPKVLHTRLLDTLTTAILLVDANMRVSYLNPAAETLLGLSRGR
                     VLRENIARIVQEDDDTIAGLQRVLATGIGFTKRQATFHLGPNHTITVDYAVTPLTLET
                     ESLVVEIRPLDRLLRISREEAILSSQQMTQVMVRGLAHEIKNPLGGLRGAAQLLAREL
                     DSEHLLDYTNVIIEEADRLRNLVDRLLSTPRRLLRQPVNIHEVLERVRQLVEAETQGE
                     LPIQRDYDPSIPELMGDREQLIQALLNVARNAQQALCDTRAPEIRFRTRTQRQFTIGN
                     QRHRLVCQIDICDNGPGVPPDILDNIFFPMISGRAEGSGLGLAIAQGIISQHHGLIEC
                     HSKPGNTVFSILIPLENSND"
     gene            5769..7190
                     /gene="ntrC"
                     /locus_tag="PLW13_07240"
     CDS             5769..7190
                     /gene="ntrC"
                     /locus_tag="PLW13_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HQY70204.1"
                     /translation="MTDTPRVWIVDDDRSIRWVLEKALAGAAIEASSFADAEALMRRL
                     RDDAPDAIISDIRMPGIDGLQLLSRIRASHPELPVIITTAHSDLDSAVASYQGGAFEY
                     LPKPFDIDEVVTVTKRALAHRRQQGEGPAEEVPPESTEIIGEAPAMQEVFRAIGRLSH
                     SLITVLINGESGTGKELVARALHRHSPRANGPFIALNMAAIPKDLMESELFGHEKGAF
                     TGAQAQRRGRFEQADGGTLFLDEIGDMPAETQTRLLRVLADSEFYRVGGHVPVHVNVR
                     IIAATHQNLEHLVREGRFREDLYHRLNVIRIHLPGLRDRREDIPRLARHFLKRAADEL
                     GGEAKLLLPETEAYMAGLSWPGNVRQLENACRWLTVMASGREVHIADLPPELLAAEQG
                     ESSRGDWVEMLRAWADSEVRRGNGRLLDTALPRFEAAMIETALMHTHGRRGEAAELLG
                     WGRNTLTRKIKELELTGLPDNGD"
     gene            7246..7710
                     /gene="trmL"
                     /locus_tag="PLW13_07245"
     CDS             7246..7710
                     /gene="trmL"
                     /locus_tag="PLW13_07245"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HQY70205.1"
                     /translation="MLDVVLYQPEIPPNTGNVIRLCANTGCALHLIEPLGFTLDDAKL
                     RRAGLDYHEWATVTTWRDFPSYLGQHPEQRVFAFSTRGATRYDRAAFRAGDALLFGPE
                     TRGLPQDVLDALSRERVLRLPMRHGSRSMNLSNTVAVAVFEAWRQLGYEGGG"
     gene            complement(7838..8302)
                     /gene="secB"
                     /locus_tag="PLW13_07250"
     CDS             complement(7838..8302)
                     /gene="secB"
                     /locus_tag="PLW13_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQY70206.1"
                     /translation="MTEQTQQQFQLQRIYVKDVSFETPQGHEVFRQQWKPRVNLEMNT
                     KQNRIDDSNYEVVLTLSVTASQEDKTAFLAEVQQAGIFFVAGIPEPQLRQVLSTVCPN
                     ILFPYAREAIDNLVTRGSFPPLMLAPVNFDALYQQAVKQQAQEQAAAAPAIN"
     gene            complement(8338..8610)
                     /gene="grxC"
                     /locus_tag="PLW13_07255"
     CDS             complement(8338..8610)
                     /gene="grxC"
                     /locus_tag="PLW13_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQY70207.1"
                     /translation="MALVTMYTTPWCPYCIAARELLQRLGVEYEDINVGSDRALRAEM
                     EMRAGRYTVPQIWIGSRHVGGFDDMNELHHRGLLEPLLEEMARGGD"
     gene            complement(8615..9031)
                     /locus_tag="PLW13_07260"
     CDS             complement(8615..9031)
                     /locus_tag="PLW13_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003220772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY70208.1"
                     /translation="MDLFIQFVGAKWYLFTAVIVLLFLLVNHERRKGGPSVSPAQLTQ
                     LVNQHEGVVVDLREAADYRQGHIVESINLPLAKLPERIAELERYRDKPVVLVCKFGQS
                     ANGAAKSLKEKGFTRVFKLGGGISEWQGSQLPLVRS"
     gene            9319..10737
                     /locus_tag="PLW13_07265"
     CDS             9319..10737
                     /locus_tag="PLW13_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQY70209.1"
                     /translation="MLQRSRMLAFALPFAVRRLALALTLGAGLAAGPARAQEAETAVS
                     PDPATAEALPRDDQPGQLPLTELRIFADVFNQIRTAYVEEVDDRTLLESAIKGMLNGL
                     DPHSAYLDKDSFADLREHTTGEFGGVGLEVGVDSNGFVRVITPIDDTPASAAGIESGD
                     VIIKLDGKSVKGLGLNDAVNMMRGPKGSKIEMTIMREGTPAPFDVTLTRDVIKVKSVR
                     ARLLEPGFGYLRIAQFQNETGADMLAAIKRMQKESKGPLRGVVLDLRNNPGGVLQSSV
                     DVADAFLESGLIVYTEGRVENARSRFSATSGDELGGAPLVVLINGGSASASEIVAGAL
                     QDHKRAMVVGTDSFGKGSVQTVLPISEERAIKLTTARYFTPKGRSIQAQGIIPDIVVE
                     RAEVTTIASTPRVTEADLARHLSNDNGNGENGSRERSTPKVSIDLRSDDNQLYEALNL
                     LKGIAFTRGSEPPASPPEPPAG"
     gene            10906..11130
                     /locus_tag="PLW13_07270"
     CDS             10906..11130
                     /locus_tag="PLW13_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HQY70210.1"
                     /translation="MDEKRNGEAGAVPMRTDRFFAVNSAWYFATREGASIGPFGDKSE
                     AQKGLDDFLDFIQLAEPKVLSTFYASLHTA"
     gene            complement(11143..11916)
                     /gene="hisF"
                     /locus_tag="PLW13_07275"
     CDS             complement(11143..11916)
                     /gene="hisF"
                     /locus_tag="PLW13_07275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQY70211.1"
                     /translation="MGLAKRIIPCLDVDKGRVVKGVRFVDIRDAGDPVEIAKRYNEQG
                     ADEVTFLDITASHEGRDTTLATVERMASEVFIPLTVGGGIRELQDIRNLLNAGADKVA
                     INTAAIHRPEFVQEASDRFGAQCIVVAIDAKKVSAHGEPLRWEIFTHGGRRATGIDAI
                     EWARRMVAYGAGEILLTSMDRDGTKDGFDIALTRTVSEAVAVPVIASGGVGSLQHLVD
                     GVLDGHADAVLAASIFHFGEFTIGEAKAYMASRGVEVRL"
     gene            complement(11919..12662)
                     /gene="hisA"
                     /locus_tag="PLW13_07280"
     CDS             complement(11919..12662)
                     /gene="hisA"
                     /locus_tag="PLW13_07280"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQY70212.1"
                     /translation="MLIIPAIDLKDGACVRLRQGRMDDSTVFGDDPVAMAARWVEEGA
                     RRLHLVDLNGAFAGEPVNGAAVRAITKAFPDLPVQIGGGIRSRETVQSYLDAGVQWVI
                     IGTLAVKDPGFVAQLCAEFPGRVIVGIDARNGMVATDGWAEVSRVAAVDLAQQFAAAG
                     VAALVYTDIDRDGMMQGVNIPATRHLAEAGGIPVIASGGVTTIEDIKALSREAASGIV
                     GAISGRALYEGTLDLRTAQAWCDANAAGG"
     gene            complement(12656..13312)
                     /gene="hisH"
                     /locus_tag="PLW13_07285"
     CDS             complement(12656..13312)
                     /gene="hisH"
                     /locus_tag="PLW13_07285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQY70213.1"
                     /translation="MQRVAVVDYGMGNLHSVAKALQHVAPGVEVLVTGDAAIIAGADR
                     VVFPGVGAIRDCMGELTRRGLAGAIRQAADEKPLLAICIGMQALMQSSEENGGTACLG
                     ILPGRVRHFGTSLADADGNTLKVPHMGWNQVRQEYHPLWTGIPQDSRFYFVHSYYVEP
                     AQRELVAGGCEYGLGFAAALARGNLFAVQFHPEKSHTAGLQLLANFVAWNGRALECAC
                     "
     gene            complement(13323..13916)
                     /gene="hisB"
                     /locus_tag="PLW13_07290"
     CDS             complement(13323..13916)
                     /gene="hisB"
                     /locus_tag="PLW13_07290"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQY70214.1"
                     /translation="MTERTAEISRNTLETKISVRLNLDGSGKAELDTGIPFLDHMLDQ
                     VARHGLVDLRVQAEGDLHIDDHHTVEDIGITIGQAFAKAIGSKAGVVRYGHSYVPLDE
                     ALSRVVIDLSGRPGLEFHVPFTRAQVGGFDVDLFREFFQGFVNHAGVTLHIDNLRGVN
                     SHHQAETVFKAFGRALRMAVTPDARMAGVTPSTKGTL"
     gene            14050..>16020
                     /locus_tag="PLW13_07295"
     CDS             14050..>16020
                     /locus_tag="PLW13_07295"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQY70215.1"
                     /translation="MNPLLKWILTAVVALLLLVGGATVALRVLVDEERLEATLVELVN
                     GNVDATLAIDGGVKLSLWPTLALAADGVRLRTPAGAGEDVATARLLRLGVALLPLLRN
                     ELRVSELVLDGLTLNAACDANATCNWAALAGTGQQEQAPPPADVAAGGATAPMLLNIA
                     RIRVRDSTLVYTDREAGARYELAGLALDGEDINMDGRPFRLEGGATLTLGAERRELKI
                     ALELPAAAIDANARTLAVPALKLAASGMELDAAVNADWSQRGAATASGTLDLKKLDLP
                     ALLQTLGTALPPAVNAAPLADITLGSRFAWAGDKLTLDELKLRAGSFSGAGSAKLGLG
                     GATRIEATISSPELDLDYFLPPQEAPPPATTSAPPAAGAPAEAQGLAALLTVDGNINA
                     KLGRLKRGKLELSDIDARLQMSRGSAQLQKLDARLYGGTLNATGALAATTDTGSMNVS
                     AQVAGVDLQALLASTQQKQQFAGRANGSLTLAATGADPATWKNTLSGPVQLGIDEPVL
                     KDLSVEQVICRAAAQLNQEALTAHFAPDTHLRSIRAALDFSQGVGHIRQLHAAVPDME
                     MRGEGRIDLPRRKLDIQLNTRVTDDLGKLDHACRMSRKMLAIEWPVSCKGRFDEPPKK
                     WCGIDKDDVVLLAAPAATEKVQDKLMKKLGDLL"
BASE COUNT         2725 a         5596 c         5241 g         2458 t
ORIGIN      
        1 tctcgggaaa cagtgtcagc atggcaaggc ccccggcggt ggcagcgatg ccgcgcattc
       61 tacgcgagcg cgggctcgta ggtcggctgt aggtcgggct tcagcccgac aagggttcgg
      121 caaagccgaa cccgctgcat tgccgcaggc acaagtcggc acgaatgccg acccacgcag
      181 tgccgaccca cccacagtgc cgagctattc ggaggcttcc cggcgtccgg cgcggcggcg
      241 ctccacttcc ttcagcagct tcttgcgcag gcgcacgctc tgcggcgtga cctcgaccag
      301 ttcgtcctcg tcgatgaagt ccagcgcctg ctcgagcgac atgcggatcg gcggcgtcag
      361 caggatgttc tcgtcggaac ccgcggcgcg cacgttggtc agctgcttgc ccttggtcgg
      421 attcaccgcg aggtcgttgt cgcggctgtg gatgccgatc agctggccct cgtagacctc
      481 gacgttcggc ccgatgaaca gccgcccgcg ctcctgcagg ctgaacagcg cgtagccaag
      541 caccttgccg gcgaccatcg acaccagcac gccgttctgg cgcgcggcga cttccccggc
      601 cttcaccggt ccgtagtgat cgaagatgct gttcagcacg ccggtgcccg aggtcatggt
      661 caggaactgg ttgcgaaagc cgatgatccc gcgcgagggg atcatgtact ccaggcgcac
      721 gcggcccttg ccgtcgggca ccatgtcacg cagctcgccg cggcgcttgc ccatctcttc
      781 catgatcgcg ccctggtgcg tctcctcgca gtcgatcagc agcgactcgt agggctcctg
      841 ctcgacgccg tcgatcacgc gccggatcac ctcggggcgc gagacgccca tctcgaagcc
      901 ctcgcggcgc atcttctcga tcagcaccga gaggtgcagc tcgccgcggc ccgagaccac
      961 gaagcggtcc ggcgtgctgc cgtcctgcac gcgcagagcc acgtcggaca acaactcgcg
     1021 ctcgaggcgg tcacggatgt tgcggctggt cacgaactgg ccctcgcgac cggcgaaggg
     1081 cgaatcgttg acctggaagg tcatgctcac ggtgggctgg tcgatggtca gcaccggcag
     1141 cgcctcgggc gcggccgggt cgcacagcgt cgaggaaatc ttcaggttgt cgatgccggt
     1201 gatgctgacg atgtcgcccg cctcgccttc ggcgatctcg gctttctcca tgcccatctg
     1261 gccgagcacc gaattgatgc gcgcattgcg gcgcgtgccg tcggcctcga tcaccaccac
     1321 ctgcatgccc ggccttgcca cgccgcgttt cacgcgcccg atgccgatca cgccgacgta
     1381 gctggagtag tccagcgcgc agatctgcat ctggaacggc ccattgctgt cggcgggcgg
     1441 cgggggcacg cgcgcgacga tcatctcgag cagcggcgac atgtcctcgg cgagctggtc
     1501 cggctcgggt ccggccacac cgttcagcgc cgaggcgtag atcaccggga agtcgagctg
     1561 ctcgtcggtc gcgccgaggc gatcgaagag gtcgaacacc tggtccatca cccagtgcgg
     1621 gcgcgcaccc gggcggtcga ccttgttcac gaccacgatc gggttcaggc ccgcttcgaa
     1681 ggccttgcgc gtcacgaagc gcgtctgcgg cataggcccg tctaccgcat cgaccagcag
     1741 cagcacgctg tcgaccatcg acaggatgcg ctcgacctcg ccgccgaagt cggcgtgtcc
     1801 gggggtgtcg acgatattga tgcgatagcc tttccagtcg atcgcggtgt tcttggccag
     1861 gatcgtgatg ccacgctcgc gctcctggtc gttggagtcc atgatgcgtt ccttgcccgc
     1921 atcacgccgc ccgagggtgc cgcattgctg gaacagcttg tcgaccaggg tggtcttgcc
     1981 atgatcgacg tgggcgatga tggcgatatt gcgcaaatgt tcccgcacag gaagcctcga
     2041 tgaagtaggg tgaatgtgaa gaggggggaa aaaccgggcg cgcagtatag ccaatcgcct
     2101 gttgccgatc cggttattaa tgcagatacg cgcggtgata tattcgcacc acggacgcat
     2161 cgcaccgtat tggtgccatg cgccccaact tggagcactg ttgcgcgcca aacgggcgcc
     2221 gccctgtgaa aaacgccggt ttgagcagcc acttcggggg caactcgcat ggcacgttga
     2281 ttgcttgact cgatcaagcc tgcgtgcggc tcaaagcgcc ggcgcgacta gcttgcagac
     2341 tcgctgtaac atcccgggcc cacagcccct tacttcccgc tcggaggaaa ccatgtcgga
     2401 acagactctc aaactgatca aggaaaacga cgtcaagtgg gtcgatcttc gattcaccga
     2461 cacgaagggc aaggaacagc acctcacgat cccctccagc gaagtgtccg ccgacttctt
     2521 cgaggacggc aagatgttcg acggcagctc cattgcgggc tggaaggcga tcaacgcctc
     2581 cgacatgctg ctgatgcccg acgacaagag ctcggtgctc gatcccttct tcgaggaatc
     2641 ggccgtgatc ctgcgctgca acgtcctcga gcccgacacc aagcagggct acaaccgcga
     2701 cccgcgctcc atcgcggcgc gcgccgagga gtacatgcgc tcgaccggta tcgccgacag
     2761 cgcgctgttc ggaccggaac ccgagttctt cgtctttgac gacgtgaaat ggcatgccga
     2821 catcagcggc gcgatgtacc agatcttttc cgaggaagcg gcctgggcct ccaagcacag
     2881 cttcgacgac ggcaacatcg gccaccgtcc cggcgtgaag ggcggctact tccccgtgcc
     2941 gccggtcgat tcgctgcagg acctgcgcgc cgcgatgtgc cgcgccatgg agaagatggg
     3001 cctcaccatc gaggtgcacc accacgaagt gggcacggcg ggccagtgcg agatcggcgt
     3061 cggccccaac gcgatcgtgc agaaggccga cgaggtgcag atcctcaagt atgccgtgca
     3121 caacgtcgct cacgcctacg gcaagaccgc caccttcatg ccaaagccgc tggtcggtga
     3181 caacggctcg ggcatgcacg tgcacatgtc gatttccaag ggcggcaaga acctgatggc
     3241 cggcgacggc tacgcaggcc tgagcgaaac cgcgatctac atgatcggcg gcatcatcaa
     3301 gcatgcccgc gccatcaacg ccttcaccaa ccccggcacg aactcctaca agcgcctggt
     3361 gcctggctac gaagcgccgg tgatgctggc ctactcggcc aagaaccgct cggcctcgat
     3421 ccgcatcccc gcggtcgcca acccgaaggc gcgccgcatc gaggtgcgct tccccgaccc
     3481 gagcgcgaac ccctacctgg gcttcgcggc actgctgatg gccggcctcg acggcatcca
     3541 gaacaagatc caccccggcg atgcgctgga caagaacctc tacgacctgc cggccgaaga
     3601 agcggcacag gtgccgacgg tgtcgcacag cctcgagatg gcgctcgccg ccctgaacgc
     3661 cgaccgcgcc ttcctcacgg caggcggcgt gttcgatgac gacatgatcg atgcctacat
     3721 cgagctgaag caggaagata tcgaccgcat ccgcatgagc acgcacccgg tcgagttcga
     3781 catgtactac agctgctgat cgcggttcgc cgccagcaaa cccgcacccg caagggtgcg
     3841 ggtttttttg tgtccgcaga tccgctgccg aacgctggcg gccacacggg gttcgcgcta
     3901 taagaatccc gagttccagc ctgcggagat ccgccgtgac cacccgaacc cgttgcgccc
     3961 gcgcactagt gatcgcatgt ctcgccgcca cggtcgccgc caccgcgctg gcgcagggcc
     4021 aggtctacaa gagccgtgac gcgcagggca acgtgatctt cagcgacacg tcgagcgagc
     4081 gcgcaagccc ggtcaccgtg ccgccgacca acaccattcc cgtagtcgcc gctccggagc
     4141 gcccggtggc gccgcccgca acggccgaca ccacggccgc cgccggctac gcgctcgcga
     4201 tcgtgacgcc ggtcgagggg cagacggtat tcagcgccgt cggggcgctg gatgtaagcc
     4261 tcagcgtgag ccccgcgctg cagcccgggc acagcctgcg cctgctgctg gacggagcgc
     4321 ccgccggcct gccgcaacag ggctcgctca cgctcgaagg gctcggcccg gggccccaca
     4381 cgctgcaggc acaggtggcg gatagcaatg gcgagatcgt gcagagctcg ggcaccgtaa
     4441 cggtgcagat gcagcgttcg cagggtcggc gcatcgggcc ggtccggatt ccctagcgca
     4501 acgagagccg gtaattcagc cacgcccgag cgcgcgctaa cgccgggcgt gctcactcac
     4561 gctatcgtcg caccatttcg gtgcatataa tctccagcgt cttccgcctg gctgcgcgac
     4621 aagccccgcc gcgccgcgat tttcgctact caccagaagc tggctcggtt cttgctgacc
     4681 caatgagtca aaggaagcca atagtcccgc catgcgcccg aaggttctgc acacccgact
     4741 gctcgacacg ctgaccaccg cgatcctgct ggtggatgca aacatgcgcg tgagctacct
     4801 caatcccgcg gcggaaacgc tgctcggcct gagtcgcggc cgcgtgctgc gcgagaacat
     4861 cgcgcgcatc gtgcaggagg acgacgacac catcgccggc ctgcagcgcg tgctggcgac
     4921 cggcatcggc ttcaccaagc gccaggccac attccacctg ggacccaatc acaccatcac
     4981 cgtggattac gcggtcacgc cactcaccct ggaaaccgag tcgctcgtgg tcgagatccg
     5041 cccgctcgac cggctgctgc gcatcagccg cgaagaggca atcctgtcct cgcaacagat
     5101 gacgcaggtg atggttcgcg gtctcgcgca cgagatcaag aacccgctgg gcggattgcg
     5161 cggggccgcg caactgctgg cgcgcgagct cgattccgag cacctgctcg actacaccaa
     5221 cgtgatcatc gaggaggccg accggctgcg caatctcgtc gaccgcctgc tcagcacgcc
     5281 acggcgcctg ctgcgccagc cggtcaacat ccacgaggtg ctggagcgcg tgcgccagct
     5341 cgtcgaggcg gaaacgcagg gcgagctgcc catccagcgc gactacgacc cgagcatccc
     5401 cgagctcatg ggcgaccgcg agcagctgat ccaggcgctg ctcaacgttg cgcgcaacgc
     5461 ccagcaggcg ctgtgcgaca cgcgcgcgcc cgagatccgc tttcgcaccc gcacccagcg
     5521 ccagttcacc atcggcaacc agcgccaccg cctggtgtgc cagatcgaca tctgcgacaa
     5581 cggcccgggc gtgccgccgg atatcctcga caacatcttc ttcccgatga tcagcgggcg
     5641 cgccgaaggc tcgggcctgg gcctcgcgat cgcccagggg atcatcagcc agcaccacgg
     5701 cctgatcgaa tgccacagca agcccggcaa caccgtcttc tcgatactga ttccgctgga
     5761 gaattccaat gactgataca ccgcgcgtgt ggatcgtgga cgacgaccgc tcgatacgct
     5821 gggtgctgga aaaggcgctc gcgggcgccg cgatcgaagc ctccagcttc gccgatgccg
     5881 aagcgttgat gcgccggctg cgcgacgacg cgccggacgc gatcatcagc gacatccgca
     5941 tgcccgggat cgacgggctg caactgctct cgcgcatccg cgcctcgcac cccgagctgc
     6001 ccgtgatcat caccacggcg cattccgacc tcgacagcgc ggtggcctcg taccagggcg
     6061 gcgccttcga gtacctgcca aagcccttcg acatcgacga ggtggtcacc gtcaccaagc
     6121 gcgcgctggc gcatcggcgc cagcagggcg aagggcccgc cgaggaagtc ccgcccgaat
     6181 cgaccgagat catcggcgag gcgccggcaa tgcaggaggt gttccgcgcc atcggtcgcc
     6241 tgtcgcattc gctgatcacg gtgctgatca acggcgagtc gggcaccggc aaggaactgg
     6301 tggcgcgcgc gctgcatcgc cacagcccgc gcgccaacgg cccgttcatc gcgctgaaca
     6361 tggccgcgat cccgaaggac ctgatggaat cggagctgtt cggccacgag aagggcgcct
     6421 tcaccggcgc gcaggcgcag cggcgcgggc gcttcgaaca ggccgacggc ggcacgctgt
     6481 tcctcgacga gatcggcgac atgcccgccg agacccagac gcggctgttg cgcgtgctgg
     6541 ccgacagcga gttctaccgc gtcggcggcc acgtgccggt gcacgtgaac gtgcgcatca
     6601 tcgcggcaac gcaccagaac ctcgagcacc tggtgcgcga gggacgcttt cgcgaggatc
     6661 tctaccatcg cctcaacgtg atccgcatcc acctgcccgg gctgcgtgac cggcgcgagg
     6721 acatcccgcg cctggcgcgg catttcctga agcgcgccgc cgacgaactg ggcggcgaag
     6781 cgaagctgct gctgcctgaa accgaggcct acatggccgg actgtcgtgg cccggcaacg
     6841 tgcgccagct ggagaatgcc tgccgctggc tgaccgtgat ggcgtcggga cgcgaggtgc
     6901 acatcgccga cctgcccccg gagttgctcg ccgcggaaca gggcgaaagt tcgcgcgggg
     6961 actgggtgga aatgctgcgc gcctgggccg acagcgaggt gcgccgcggc aacggccgac
     7021 tgctcgacac cgcgctgcca cgtttcgagg ccgcgatgat cgagacggcg ctgatgcaca
     7081 cgcacgggcg gcgcggcgaa gccgccgagc tgctcggctg gggccgcaac acgctgacgc
     7141 gcaagatcaa ggaactggag ctcaccgggc tgcccgacaa cggcgactga cgggcgcgat
     7201 acgctgcgct ggtcgcgggc gtagcccgct cctacaatcg cggccatgct cgacgtcgtg
     7261 ctctaccaac ccgaaatccc gcccaatacc ggcaacgtga tccggctgtg cgccaacacc
     7321 ggctgcgcgc tgcacctgat cgagccgctc ggcttcacgc tcgacgacgc gaagctgcgt
     7381 cgcgccgggc tcgactacca cgagtgggca acggtcacga cctggcggga cttcccctcc
     7441 tatctcggac agcacccgga acaacgcgtg ttcgcgttca gcacgcgcgg cgccacgcgc
     7501 tatgaccgtg cggcgtttcg cgccggcgat gcgctgctgt tcggcccgga aacgcgcggc
     7561 ctgccgcagg acgtgctgga tgcgctgtcg cgcgagcgtg tgctgcgcct gccgatgcgt
     7621 cacggcagcc gcagcatgaa cctgtcgaac acggtcgccg tggccgtgtt cgaggcctgg
     7681 cggcagctcg gctacgaggg gggaggctga gcgggagtcc tgtcgcgggc gtggcccgct
     7741 cctgcgggcg ggggcgctgg acgcgtcgcg ggcatggccc gctcctacga cggggcgcat
     7801 ccgtaggagc gggccatgcc cgcgacatca ggcagcctca gttgatggcg ggcgctgcgg
     7861 cggcctgttc ctgcgcctgc tgcttcaccg cctgctggta cagcgcgtcg aagttcaccg
     7921 gcgcgagcat gaggggaggg aagctgcctc gggtcaccag gttgtcgatg gcctcgcgcg
     7981 catacgggaa caggatgttc gggcacacgg tgctcagcac ctggcgcagc tgcggctcgg
     8041 gaatgccggc aacgaagaag atgccggcct gctgcacctc ggcgaggaac gcggtcttgt
     8101 cttcctgcga ggcggtgacg gacagcgtca gcaccacttc gtagttgctg tcgtcgatgc
     8161 ggttctgctt ggtgttcatc tcgaggttca cgcgcggctt ccactgctgg cggaacactt
     8221 cgtggccctg gggcgtctcg aaggacacgt ccttcacgta aatgcgctgc agctggaact
     8281 gctgctgggt ctgctcggtc atcgatcggt cctctcggtg gtgttgtgca ttgctgatca
     8341 gtcgccgccc cgggccattt cctcgagcag cggttcgagc aatccccggt gatgcagctc
     8401 gttcatgtca tcgaagccgc cgacatggcg actgccgatc catatctgcg gcaccgtgta
     8461 gcggccggcg cgcatttcca tctcggcgcg cagcgcgcgg tccgagccca cgttgatgtc
     8521 ctcgtactcg acgcccagcc gctgcaacaa ctcgcgcgcg gcgatgcagt agggacacca
     8581 cggcgtggtg tacatcgtga ccagcgccat cgcttcagga tctcaccagc ggcagctgcg
     8641 agccctgcca ctccgagatg ccgccgccga gcttgaagac cctggtgaag cccttctcct
     8701 tcagcgactt cgcggcaccg ttggcggact ggccgaactt gcagaccagc accaccggct
     8761 tgtcgcggta gcgctcgagc tccgcgatgc gctcgggcag cttcgccagc ggcaggttga
     8821 tgctctcgac gatatgaccc tggcgatagt ccgccgcctc gcgcaggtcg accacgactc
     8881 cctcgtgctg gttcacgagc tgcgtgagct gggccggcga gacgctgggg ccgcccttcc
     8941 tgcgctcatg gttcacgagc aggaacagca gcacgatgac tgccgtgaac aggtaccact
     9001 tggcacccac gaactggatg aacagatcca ttgaatcccc gggccaggcc caatcgcggc
     9061 gcgcgctccg ttcgcggaaa acgccccaga aacaaagcgc gcaagtatac acgccgcccc
     9121 ggcggcccac caaccggccc tggccggccc ggcgaggggc gggcccctcg ccgcgcctcc
     9181 cgtcggtggt tcatggtccc cgtgggagtg cgccctgcgc gcgacagggc ggcagttcgt
     9241 cctatggtgc cggcaatccg cttcatgcaa actggccgta catattttgt gctttccgga
     9301 accggttcac tgccctttat gctccaacgt agtcgtatgc tcgcgtttgc cctgcctttc
     9361 gccgttcgcc gccttgccct ggcactgacc ctcggtgccg gcctcgcggc cggcccggct
     9421 cgcgcgcagg aagccgagac ggcggtcagc cccgacccgg ccacggcgga ggcgctgccg
     9481 cgcgacgacc agccggggca gctgccgctg acggaactgc gcatcttcgc cgacgtgttc
     9541 aaccagatcc gcaccgccta cgtggaggaa gtcgacgacc gcaccctgct cgagagcgcg
     9601 atcaagggca tgctgaacgg gctggacccg cactcggcct acctcgacaa ggattccttc
     9661 gccgacctgc gcgagcacac cacgggcgag ttcggcggcg tggggctgga ggtcggcgtc
     9721 gacagcaacg gcttcgtgcg cgtgatcacc ccgatcgatg acacgcccgc gagcgcggcg
     9781 ggcatcgaga gcggcgacgt gatcatcaag ctcgacggca agtcggtcaa aggcctgggg
     9841 ctgaacgatg ccgtgaacat gatgcgcggg ccgaagggct cgaagatcga gatgacgatc
     9901 atgcgcgagg gcacccccgc ccccttcgat gtcacgctga cgcgcgacgt tatcaaggtg
     9961 aagagcgtgc gcgcgcgcct gctggagccg ggcttcggct acctgcgcat cgcgcagttc
    10021 cagaacgaga ccggcgccga catgctggcc gccatcaagc gcatgcaaaa ggagagcaag
    10081 ggcccgctgc gcggcgtggt gctggacctg cgcaacaacc cgggcggcgt gctgcagtcc
    10141 tcggtcgatg tggccgacgc tttcctcgaa tcggggctca tcgtctacac cgagggccgc
    10201 gtcgagaacg cgcgctcgcg cttcagcgcc acgagcggcg acgagctcgg cggagcgccg
    10261 ctggtggtgc tgatcaacgg cggctcggct tccgcctcgg aaatcgtggc cggcgcgctg
    10321 caggaccaca agcgcgcgat ggtggtcggc accgacagct tcggcaaggg ctcggtgcag
    10381 acggtgctgc cgatcagcga ggagcgcgcg atcaagctca ccaccgcgcg ctacttcacg
    10441 ccgaaaggcc gctcgatcca ggcccagggc atcatccctg acatcgtggt cgagcgcgcc
    10501 gaggtcacga ccatcgccag cacgccgcgg gtcaccgagg cggacctcgc gcgccacctc
    10561 tcgaatgaca acggcaacgg cgagaacggc agccgcgagc gcagcacgcc gaaggtcagt
    10621 atcgacctgc gcagcgacga caaccagctc tacgaggcgc tgaacctgct gaagggaatc
    10681 gcgttcaccc gcggctccga gccgcccgcg agcccgcccg aaccccccgc gggttgagcc
    10741 gcgacggggg ctctaccggg gttgcgcggg acaaagatcg gcagtatttg acaaacccgg
    10801 aatattagtg cgaacttcgt cacacttccg acagcctgtt ctagactgca accggaagcg
    10861 gataggcata tccgagcgaa acaactggcc tgggtgcaca agaaaatgga cgaaaagcgc
    10921 aacggagaag ccggcgcggt gccgatgcgg acggatcgtt tttttgccgt caacagcgcc
    10981 tggtatttcg cgacccgcga gggggcatcg attggcccct tcggcgacaa gagcgaggcc
    11041 cagaaaggac tcgacgactt cctggacttc atccagctcg cggaacccaa ggtgctgtcg
    11101 accttttacg cctcgctgca cacggcgtga cgggcgcgct gcctaaagcc tgacctccac
    11161 cccgcgggag gccatgtagg cctttgcctc cccgatcgtg aattccccga agtgaaagat
    11221 gctggcagcc agcacggcgt ccgcatgccc gtcgagcacg ccgtcgacca ggtgctgcag
    11281 gctgccgacc ccgcccgagg cgatcaccgg caccgccacc gcctcgctga ccgtgcgcgt
    11341 cagcgcgata tcgaagccgt ccttcgtgcc gtcgcggtcc atgctggtca gcaggatctc
    11401 gcccgcgccg taggcaacca tgcgccgcgc ccattcgatc gcgtcgatgc cggtcgcgcg
    11461 gcgcccgccg tgggtgaata tctcccagcg caacggctca ccgtgagcgc tcactttctt
    11521 ggcatctatc gccaccacga tgcactgcgc cccgaaacgg tcggaagcct cctgcacgaa
    11581 ctccgggcgg tggatcgccg cggtattgat cgcgaccttg tcggcgccgg cgttcaggag
    11641 gttgcggatg tcctgcagtt cgcggatgcc gccgcccacg gtcagcggga tgaacacctc
    11701 gctggccatg cgctccacgg tggcgagcgt ggtgtcgcgg ccctcgtggc tcgcggtgat
    11761 gtcgaggaag gtgacctcgt cggcgccctg ctcgttgtag cgcttggcga tctccaccgg
    11821 gtcgccggca tcgcggatat ccacgaagcg cacgcccttg accacgcgcc ccttgtcgac
    11881 gtcgagacac ggaatgatgc gcttggccag ccccatgctc agccccccgc ggcgttcgcg
    11941 tcgcaccagg cctgcgcggt gcgcaggtcc agcgtgccct cgtacagcgc gcggccgctg
    12001 atcgcgccga cgatcccgct cgcggcctcg cgcgacagcg ccttgatgtc ctcgatggtg
    12061 gtcacgccgc ccgaggcgat gacgggaatg ccgccggcct cggccaggtg gcgcgtcgcc
    12121 gggatgttca cgccctgcat catgccgtcg cggtcgatgt cggtgtagac gagcgcggcc
    12181 acgcccgcgg ccgcgaactg ctgcgccagg tcgaccgcgg caacgcgcga aacctcggcc
    12241 cagccgtcgg tggccaccat gccgttgcgt gcatcgatgc cgacgatcac gcggcccggg
    12301 aactcggcgc agagctgcgc gacgaacccg ggatccttca ccgccagcgt gccgatgatc
    12361 acccactgca cgcccgcatc gaggtagctc tgcacggtct cgcgcgagcg tatgccgccg
    12421 ccgatctgca ccggcagatc cggaaacgcc ttcgtgatcg cgcgcaccgc agcgccgttg
    12481 accggctcgc ccgcgaaggc gccgttcagg tccacgaggt gcaggcgccg cgcgccctct
    12541 tccacccagc gcgcggccat cgcgaccgga tcgtcgccaa agacggtcga atcgtccatg
    12601 cgcccctggc gcaggcgcac gcaggcgccg tccttgaggt cgatggcggg gatgatcagc
    12661 atgcgcactc cagtgcccgc ccgttccagg cgacgaaatt cgcgagcaac tgcagccccg
    12721 cggtatgact tttttccggg tggaactgca ccgcgaacag gttgccgcgc gcgagcgccg
    12781 ccgcgaaccc gaggccgtat tcgcagccgc cggccaccag ctcgcgctgt gccggttcca
    12841 cgtagtagct gtgcacgaag tagaagcggc tgtcctgcgg gatgccggtc cacagcggat
    12901 ggtattcctg gcgcacctgg ttccagccca tgtgcggcac cttcagcgtg ttgccgtcgg
    12961 cgtccgcgag cgaggtgccg aaatggcgca cgcgcccggg caggatgccg aggcaggcag
    13021 tgccgccgtt ctcctcgctg gattgcatca gcgcctgcat gccgatgcag atggcgagca
    13081 gcggcttttc gtcggccgcc tggcggatcg cgccggcgag gccgcgacgg gtcagctcgc
    13141 ccatgcagtc gcggatcgca ccgacgccgg ggaagaccac ccgatcggcg ccggcgatga
    13201 ttgccgcgtc gccggtgacc agcacctcca ccccgggcgc aacatgctgc agggccttgg
    13261 cgaccgagtg caggttaccc atgccgtagt cgacgaccgc gacgcgctgc atcgtcatgg
    13321 cgctagagcg tgcccttggt ggacggcgtg acgccggcca tgcgcgcatc cggcgtcacg
    13381 gccatgcgca acgcgcgacc gaaggccttg aacaccgtct cggcctggtg gtgcgagttc
    13441 acgccgcgca ggttgtcgat gtgcagcgtc acgccggcat ggttcacgaa gccctggaag
    13501 aactcgcgga acaggtcgac atcgaagccg ccgacctgcg cgcgcgtgaa cggcacgtgg
    13561 aactccagcc ccgggcgccc cgagagatcg atcaccacgc gcgacagcgc ttcgtccagc
    13621 ggcacgtagc tgtggccgta gcgcaccacg ccggccttgc tgccgatcgc cttcgcgaag
    13681 gcctggccga tggtgatgcc gatgtcctcg accgtgtggt ggtcgtcgat gtgcaggtcg
    13741 ccctcggcct gcacgcgcag gtccacgagg ccgtgccgcg ccacctggtc cagcatgtgg
    13801 tcgaggaacg gaataccggt gtccagctcg gccttgcccg aaccgtccag gttcagccgc
    13861 acgctgatct tcgtctcgag ggtgttgcgg ctgatttctg ccgtacgctc tgtcatggtc
    13921 gaaccttcgg cccggggccg ccaaatcaag accgcgatta tagagaaccg ggcccggctt
    13981 ttcagcggca cgattccgct acaatcgggc tgatgtcggg ctgaagcccg acctacgggc
    14041 gagaagcgca tgaatcccct gctgaaatgg atcctcaccg ccgtcgtcgc gctgctgctg
    14101 ctcgtcggcg gcgcgaccgt ggcgctgcgc gtgctggtcg acgaggaacg gctcgaggcc
    14161 acgctcgtcg agctggtcaa cggcaatgtc gatgccacgc tcgccatcga cggcggcgtg
    14221 aagctgtcgc tgtggcccac gctggcgctc gcggccgacg gcgtgcgcct gcgcacgccg
    14281 gccggcgccg gcgaggacgt cgccaccgcc aggcttctac gcctcggcgt ggcgttgctg
    14341 ccgctgctgc gcaacgagct gcgagtgagc gagctggtgc tcgacgggct gacgctgaac
    14401 gccgcctgcg atgccaacgc cacctgcaac tgggccgcgc ttgcgggcac cgggcagcag
    14461 gagcaggcgc cgccgcccgc cgatgtagcc gccgggggcg ccaccgcgcc gatgctgctg
    14521 aacatcgcgc gcatccgggt tcgcgacagc accctcgtct acacggaccg cgaggcgggg
    14581 gcgcgctacg agctcgccgg cctcgcgctc gacggcgagg acatcaacat ggacggccgc
    14641 ccgttccgcc tcgagggcgg cgcgacgctg acgctgggcg cggagcgacg cgaactgaag
    14701 atcgcacttg aactgccggc cgccgcgatc gacgcgaacg cgcgcacgct ggccgtgccg
    14761 gcgctgaagc tggccgcgag cggtatggag ctcgacgccg cggtgaacgc cgactggagc
    14821 cagcgtggcg ccgcgaccgc cagcggcacg ctggacctga agaagctcga cctgccggcg
    14881 ctgctgcaga ctttgggcac ggcgctgccg cccgccgtca acgccgcgcc gcttgcggac
    14941 atcacgctcg gctcgcgctt cgcctgggcg ggagacaagc tcacgctcga cgaactgaaa
    15001 ctgcgcgccg gcagcttcag cggtgcgggc agcgcaaagc tcggtctcgg cggggccacg
    15061 cgcatcgagg cgaccatcag cagccccgag ctcgacctcg actatttcct gccgccgcag
    15121 gaagcgccgc cgcccgcgac gacgtcggcg cctcccgccg caggcgcgcc tgccgaggcg
    15181 cagggactgg ccgcgctgct caccgtcgat ggcaacatca acgcgaagct cggccgtctg
    15241 aagcgcggca agctggaatt gagcgatatc gatgcccggc tgcagatgag caggggcagt
    15301 gcgcaactgc agaagctcga cgcgagactc tacggcggca cgctgaacgc caccggcgcg
    15361 ctcgccgcca cgacggatac gggctcgatg aacgtcagcg cgcaagtcgc gggcgtggac
    15421 ctccaagcgc tgctcgccag cacgcagcag aagcagcaat tcgccggtcg cgcgaacggc
    15481 tcgctcaccc tggccgcgac cggcgccgac ccggccacgt ggaagaacac cctgagcggc
    15541 ccggtgcaac tcggcatcga cgaaccggta ttgaaggacc tgagcgtcga gcaagtgatc
    15601 tgcagggccg ccgcgcagct caaccaggag gcactcacgg cgcacttcgc gcccgacacg
    15661 catttgcgca gcatccgggc cgcgctcgac ttcagccagg gcgtcggcca catccgccag
    15721 ctgcacgccg cggtgccgga catggaaatg cgcggcgagg gccggatcga cctgccgcgg
    15781 cgcaagctcg acatccagct gaacacgcgc gtcaccgacg acctcggcaa actcgaccac
    15841 gcctgccgca tgagccgcaa gatgctcgcg atcgaatggc ccgtgagctg caagggccgc
    15901 ttcgacgagc cgccgaaaaa gtggtgcggc atcgacaagg acgacgtggt tttgcttgct
    15961 gctccggcgg ccaccgagaa ggtgcaggac aagctcatga agaaactcgg cgacctcctc
//
LOCUS       DAORUQ010000061        15835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3446_length_15835_cov_6.920849, whole genome shotgun sequence.
ACCESSION   DAORUQ010000061 DAORUQ010000000
VERSION     DAORUQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15835
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_15835_cov_6.920849"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1517)
                     /locus_tag="PLW13_07300"
     CDS             complement(330..1517)
                     /locus_tag="PLW13_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70216.1"
                     /translation="MKVEFTPEQERLRQELRAYFKALITPELDAELEESPGEGGGPLW
                     KAAMRQMGRDGWIGLGWPKELGGRGMTPLEQFIFVEEVMRSGFPFPFLTSESIGPTLA
                     EHGNDYHRREIVPKILSGDLCIAIGYSEPGAGTDLASLKTSAVPDGDGWVINGQKMWT
                     SLAHISEYVWIAVRTDPDPAKRHKGISMFLIPTTDPGWSCTPVHTLGGVRTNATYYDN
                     VRVTRQNLVGELNGGWKLITGQLNRERLSLMNYGPTAVLLNTITQWAADATLANGQRL
                     VDQPWVQMNLARARAGLEALKLMCYKQAWAMTHSTLDMADASAVKVYGSEFFIELYRL
                     LGEVVGQASLVSRHSPVGQVAKARIERLYRTSSILTFGGGANEVQRDIISAAGLWMPR
                     AAR"
     gene            1785..2219
                     /locus_tag="PLW13_07305"
     CDS             1785..2219
                     /locus_tag="PLW13_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY70217.1"
                     /translation="MTAKPKVAALEGWFTLDEQAPQLLGSRCKACATVYFPKQTSFCR
                     NPACEGTEFEEAPLSRRGRVWSCTNASYQPPEPFVAPEPFVPFTIAAVELEAERMVVL
                     GQVVAGVEVTDLKVGQEMELVLDTLYTDDESEKMIWKWKPVA"
     gene            2233..3420
                     /locus_tag="PLW13_07310"
     CDS             2233..3420
                     /locus_tag="PLW13_07310"
                     /inference="COORDINATES: protein motif:HMM:NF004715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HQY70218.1"
                     /translation="MSNDIAILGVGMHPWGKWGRNFVEYGVHAARAAIADADVRWQDI
                     RFVSGAATMRCGYPGYVAGATFAQALGWQGAEVNTSYAACASGSQALAAARAKILAGE
                     CEVALVVGADTTPKGFLAPAKGYRPDDPDWVRFLVGITNPTYFALYARRRMDLYGDTE
                     DDFGLVKIKNSEHGFTNPNARYRKKFTMDDVHASPMVAHPLRLLQICATSDGGAALVV
                     SSMDYARRIGRGDAVRVAAISTVTPTFASEVVEMPDIATDSAAAAAVDTHRFRASIPL
                     KAYEEAGVAPRDVNVAEVYDLSSALELDWMEDLQLCPRGEAARMVRAGETRIGGRVPV
                     NPSGGLACFGEAVPAQALAQVCELTWQLRGQGGERQVEGAKVGITANQGLFGHGSSVI
                     VKR"
     gene            3442..4110
                     /gene="can"
                     /locus_tag="PLW13_07315"
     CDS             3442..4110
                     /gene="can"
                     /locus_tag="PLW13_07315"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HQY70219.1"
                     /translation="MKNELHNLFANNRRWAAAIRDRDPAFFETLARQQSPEYLWIGCS
                     DSRVPANEIIGLLPGEVFVHRNVANVVVHTDLNCLSVLQFAVDVLNIKHVMVVGHYGC
                     GGVAAALRNRKFGLADNWLRHVQDVQKKHAATFANAHPVDDDALLVDRLCELNVIEQV
                     QNVCQTTVVQDAWERGQDLTVHGWIYRLQDGELRDLCATAASPAEVDGAIQAAIARLT
                     PLAP"
     gene            4118..5992
                     /locus_tag="PLW13_07320"
     CDS             4118..5992
                     /locus_tag="PLW13_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY70220.1"
                     /translation="MIHLSGVALARGAKTLLADAELRIHAGERVGVIGQNGCGKSTLF
                     ALLRGEIQPDAGTASMPADWRIAAMEQEVLGTERRAIDFVLDGDTEFRALEAAMETAG
                     GEQLGEIHARFAAIDGYRAAARAHELLDGLGFATAERERAVREFSGGWRLRLALARTL
                     MCPSDLMLLDEPTNHLDLDALVWLEAWLARYPGTLILISHDRDFLDNVCNRIVAFEGG
                     TLSLYRGNYSAFERQRAEKLASQQQAFQKQQQRIQEIEGFVARFRAQATKARQAQSRL
                     KELERMTRIAPAHIDSQFTLRLPCYERVSTPLLNFERAALGYGDTVILPRVELGIMPA
                     QRIGLLGPNGAGKSTLIKTIAGELAPLAGLRHTGEHLRIGYFAQHQLEALDLAATPFL
                     HVQRLSPAASEQSIRDFLGGYGFHGDQALGTVGNFSGGEQARLALALVAWQKPNLLLL
                     DEPTNHLDLEMRHALTLALQEYPGAVLIVSHDRHLLRNTVDEFLLVADGRVEPFDGDL
                     DDYRQLLLEKRAAPAADVAAEEPGARVDRRDERRLAAEKRERLRPWRNRIASLEKDME
                     KLQAELRALDERLQDPALYEAARAGEVQDLLRRQGALKQELATVEEDWLMACEELESL
                     "
     gene            complement(6051..8633)
                     /gene="clpB"
                     /locus_tag="PLW13_07325"
     CDS             complement(6051..8633)
                     /gene="clpB"
                     /locus_tag="PLW13_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQY70221.1"
                     /translation="MRMDRLTNQLQGAIADAQSLALGRDHNFIMPAHLLHVLLEQQGG
                     TVRPLLLQCGVDVKALRQRITALLDRLPCVEGVGGEVHVSNELTKLLNLSDKFAQQRK
                     DKFISSELVLLAALEDRSGVGEALKLSGLERRALEQSIDQVRGGEGVDDPEAEGTRQA
                     LEKYTIDITRRAAEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNRRLLSLDLGALLAGAKYRGDFEERLKAVLKEIGREEGRVILF
                     IDELHTVVGAGKAEGALDAGNMLKPALARGELHCVGATTLNEYREYIEKDAALERRFQ
                     KVLVEEPNEEDTIAILRGLRERYEVHHAVTITDSAIIAATRLSQRYITDRQLPDKAID
                     LIDEAASRIRMEIDSKPEELDRLDRRLIQLKIEREAVRKDEDQSARQRLEKIEAEIAE
                     LEREYAGLDEIWKAEKATVQGSHQIKSELEQARLDMESARRAGDLTRMAELQYGIIPA
                     LEKRLAAAGELESGGHRMQLLRDKVGEEEIAEVVSKWTGIPVSKMLEGERDKLLRMEE
                     ALHRRVIGQDEAIIAVSNAVRRSRAGLSDPMRPHGSFLFLGPTGVGKTELCKALAEFL
                     FDSQEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSLLLLDEVE
                     KAHPDVFNVLLQVLEDGRLTDGHGRTVDFRNTVVVMTSNLGSDLIQEMATEADYPRMK
                     AAVMDVVGRYFRPEFINRIDDTVVFHPLGAEQIHGIAQMQLELLRRRLGERDLGLDVS
                     PAAVDLLARAGFDPVYGARPLKRAIQHRIENPLAQQILSGAFAPGSTVVVDASGEELT
                     FRAT"
     gene            8823..9578
                     /locus_tag="PLW13_07330"
     CDS             8823..9578
                     /locus_tag="PLW13_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HQY70222.1"
                     /translation="MLAALAPLLRHETRIGSNASMTSMVPPSQLSTASVPHASGNCIG
                     HFYVAKSVRMAYLSIPKSGCTSLINMLHAVAIPGFSTIGTRIHRESTLPRVPAPACPA
                     GLLRFTFVRNPYLRLLSFYRSKVLKMADPDPHVNMTLLKMGLREGMSFASMVEVLSTV
                     PPRAMDLHCAPQILFVYHIGKPVVDFVGRLESLQEDWSELEARCGVHTGLLQENMKHG
                     IDLIDEYRDPSVRARVHALYRADFETFGYASEL"
     gene            9568..10578
                     /locus_tag="PLW13_07335"
     CDS             9568..10578
                     /locus_tag="PLW13_07335"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY70223.1"
                     /translation="MNSESDALRAGFGNARVLVSGGLGFIGSNLALRLCETGAAVTLL
                     DNLAPSYGGNRFNVAPIARQAEVIIGDLRDERLMHQLVAGQDYLFNLAGQTSHIDSMQ
                     DPQQDLEANVRAQLAVLEACRKVNPGIRVVFASTRQVYGKPAYLPVDEAHPLNPVDVN
                     GVHKIAGEMYHQLYAQVYGLATTVLRLTNTYGPRMRVRDDRQTFLGTWIRRLLDGETL
                     QVFGDGRQLRDFNYVDDVVRALMLCALARDTAGKVYNLGSSEVLSLEALAQKLVATHG
                     SGSYELVAFPDERKRIDIGDYFGSSARIEHDLGWQAQVGLDAGLRQTLDYFGRFGGHY
                     WP"
     gene            10575..11681
                     /locus_tag="PLW13_07340"
     CDS             10575..11681
                     /locus_tag="PLW13_07340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY70224.1"
                     /translation="MTIPIAAPVRWFEQHQQAVAAAVAGVVSGGQYILGPRVDAFESA
                     FAAFCACPHAVAVASGTDAITLGLRVLDVAGGEVITSAHTAVATVAAIERAGATPVVA
                     DIDPVTHCLCPESVASVLSPRTRAIVAVHIFGHPADLPGLRAVSGARDIGLVEDCAQA
                     HGARIDGRPVGSFGLAGAFSFYPTKNIGALGDAGAIVTHDEHVARQLRQLRQYGWDET
                     RCSQLHGQNSRMDEVQAAILALRLADFPPAFLRRQQIAQRYNDALANHPHVLAPQTAP
                     GATHAFHLYVVRTAQPEALAAHLLAQGIQSARHYAAPIHRQPAYRDILRAKSLRNVER
                     LYETMLSIPIYPELTDREVHRICEALSSWRAGGK"
     gene            11682..12356
                     /locus_tag="PLW13_07345"
     CDS             11682..12356
                     /locus_tag="PLW13_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70225.1"
                     /translation="MVFESNRTIVLYGIGSEYVYEAVEIALRAGVTVAAFVDNLPASD
                     GRQFPGLEPVLVAQACDEGVRALPVLVPLITPGHRKQVAAEAMSLGFAGVATLIDPTA
                     ILARSTRFGDGFHVNAAVVVGANCEFGRQVLLNRSASIGHDAQIGDFASLGPGCVLCG
                     SCRIGAGAFIGGGATIAPKVSVGANAIVGAGAVVIRDVPPNCFVAGNPARIVREGVQG
                     YNDVGV"
     gene            12368..15286
                     /locus_tag="PLW13_07350"
     CDS             12368..15286
                     /locus_tag="PLW13_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQY70226.1"
                     /translation="MEILVYSQQGNMGGSTRLLLNLARHLAREHSVSVAFASHHSPQA
                     SDILLARFPELRLIPGDAQTMRRRRFDVAILHLPFAMEDVDNFKAARKIAVLMELVER
                     HPIALREEHCAQFERILHLHREQVRHLSPASRAAKCTQLPVIDNIDFEPEYVPACCIG
                     AIGGAHKMQLPLALSLLDALPQRYSMCFWSPDPLAIDGLALPLLDQALRLVSAGRLRQ
                     QSPSGDIRLLSQRYRALLHTPLHGNGTSVVVSDALSCGKLAILAPLPAYREAYGHLDG
                     VLFTDQPGTELAGRICAYSREDFSRIRAAYRAHHDRSDILRLWSAAILDGDFHPALAP
                     GREPELATTDAASAAAPRVMCATVNPHTRAPSMSEAPSRNAPCPCGSGKRYKHCCGAA
                     AQSTPVAAQAPVPEVTTEQLNLRLAHSIESYNRFDFRLAASLCDSVLASDAATALQRL
                     AAAEIGGAACTRLDRHARARELYLVALSIDPDRVQTLHNLGLNAIHLGDFALAIEYAN
                     RLLSVEPGNTNAYNTMTNALRELGRWHEAKELYARLIADGREDETVYNSYLMSLHYTP
                     MDPEEAYRAHIGWAERYAPAANMAARVHHNDRSTDRKLRLGYFSPNFSRKIAGYFIRP
                     LIAHHDRTRFDLYLYSNTAVEDDYTDYFRDNCSKWTDVRGLDAKQVAQAIVDDRIDVM
                     VDLAGHAPDNILAALSYKPAPVQITMLDYFDTTGVSAMDYFVSDDYHSPVGSPQRFVE
                     KLLRLSTIRLCYEPVDLAPPVRELPAARNGYITFGSFNRRQKVTPEVIGLWSRVLHDV
                     PGSRLVLKGSYFDQAAVQADILHHFCAEGIDAARIEFRGRSHHREALEQYGDVDIGLD
                     TFPWNGGLTTCESLLMGVPVIALAGDRIISRQSAAMMHALGLDDFVADDRDQYVDIAR
                     RWSASTGALEQLRRELRQRLLASPLGDGKRYAAAVEQIYASTWRAWCEGAQPGGRTTS
                     "
     gene            complement(15300..>15835)
                     /gene="pgeF"
                     /locus_tag="PLW13_07355"
     CDS             complement(15300..>15835)
                     /gene="pgeF"
                     /locus_tag="PLW13_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQY70227.1"
                     /translation="DGIVRTGDAAHTSAPGVVCAVMTADCLPVLLTDRAGSQVAAVHC
                     GWRGLAAGVLAATVARFACPGSELLAWLGPAISQRAFEVGAEVREAFLAPCRDESARG
                     AVRAAFLPGHDGASRYHADLYALARVALAALGVRRVYGGGFCTVGSPELFYSYRRDGL
                     TGRMASLIWISRAPGDA"
BASE COUNT         2553 a         5561 c         5231 g         2490 t
ORIGIN      
        1 cgaaatcaca gatttcgggc gctcggcggg gcgaagcagc gcttcgccgg attccgcctc
       61 gcccctgtag gctcgagcgc cgcatccctg cggcgcacgg tccgcccggg tgactcgcca
      121 ccccggcgcg caatcctgtc tgctgccacg acctgatgga gcgtggcagc agcacccagg
      181 ccgtcgctgt cattcttgct gaggggaccg tctgccgcag ggatgcggca gtcgagccta
      241 cagggacgta ttcacggcgt gtcccggcag caagaatgac agcgacggcc cgcgccaacg
      301 ctccgatgcg cgtgatcagt gcccaacgct cagcgcgccg cccgcggcat ccacaggccg
      361 gcggccgaga tgatgtcgcg ttgtacttcg ttcgcgccgc cgccgaaggt gaggatcgag
      421 gaggtgcggt agagacgctc gatgcgcgcc ttcgccacct gcccgaccgg cgaatgccgg
      481 ctgaccagcg aggcctgccc gaccacctcg ccgagcagac ggtagagctc gatgaagaac
      541 tcggagccgt agaccttcac ggcagaggca tcggccatgt cgagcgtgct gtgggtcatc
      601 gcccaagcct gcttgtagca catgagcttc agcgcctcga ggccggcgcg cgcgcgcgcc
      661 aggttcatct gcacccaggg ctggtcgacc aggcgctggc cgttggcgag cgtcgcgtcg
      721 gccgcccact gcgtgatcgt gttcagcagc accgcggtgg gaccgtagtt catcaggctc
      781 aggcgctcgc ggttcagctg cccggtgatg agcttccagc cgccgttgag ctcgccgacc
      841 aggttctggc gcgtgacgcg cacgttgtcg tagtaggtcg cgttggtgcg cacgccaccg
      901 agcgtgtgca ccggcgtgca gctccagccc gggtccgtag tagggatcag gaacatcgag
      961 atgcccttgt ggcgctttgc cggatcggga tccgtgcgca cggcaatcca cacgtattcg
     1021 ctgatgtgcg ccagactggt ccacatcttc tggccattga tgacccagcc gtcgccgtcg
     1081 gggacagcgg aggtcttcag cgacgcgaga tcggtaccgg cgcccggctc ggagtagccg
     1141 atcgcgatgc acagatcgcc cgagaggatc ttcggcacga tctcgcgacg atggtagtcg
     1201 ttgccgtgct cggccagcgt ggggccgatc gactccgagg tgaggaaggg gaacgggaaa
     1261 cccgagcgca tcacctcctc gacgaagatg aactgctcca gcggcgtcat gccgcgcccg
     1321 ccgagctcct tcggccagcc caagccgatc cagccgtcgc gccccatctg gcgcatcgcc
     1381 gccttccaca gcgggccccc gccctcgccg gggctctcct cgagctccgc gtccagttcg
     1441 ggggtgatca gcgccttgaa ataggcgcgc aactcctggc gcaggcgctc ctgttcgggt
     1501 gtgaactcga ctttcatccg catctctcct gggaccgggt gccgcgttgt tccgggggcg
     1561 gcatcgccga cgcatgctgc cgccgcgagt catatgtcgg caagcgcaaa aaaggatcag
     1621 cgcgcgctga caatagcgct caccagagcc cgttgcggtt gctggcgcgg ggccgcgggc
     1681 tgtatccttc gcgctcgctc cggcgcggca atacggcccg cgccccaccc accagcaaag
     1741 caacccggtc gcgcgcggca ggggtgcgga cgcggagaac atgcatgact gcaaaaccca
     1801 aggtcgcggc cctcgagggc tggttcacgc tcgacgaaca ggcgccgcag ctgctgggct
     1861 cgcgctgcaa ggcctgcgcc acggtctact tcccgaagca gaccagcttc tgccgcaacc
     1921 cggcctgcga gggcaccgag ttcgaggaag cgccgctgtc gcgccgcggt cgcgtgtggt
     1981 cctgcaccaa cgcctcgtac cagccgccgg aacccttcgt ggcgcccgag cccttcgtgc
     2041 cgttcacgat cgccgccgtc gagctcgaag ccgagcgcat ggtggtgctg gggcaggtcg
     2101 tggccggcgt cgaggtcacc gacctgaagg tcggccagga aatggagctg gtgctggata
     2161 cgctctacac cgacgacgag tcggaaaaga tgatctggaa atggaaaccc gtggcctgag
     2221 gagtaggcaa gcatgagcaa tgacatcgcc attctcggcg tcgggatgca cccctggggc
     2281 aagtggggca ggaacttcgt cgaatacggc gtgcacgcgg cgcgcgccgc tatcgccgat
     2341 gccgacgtgc gctggcagga tatccgcttc gtctcgggtg ccgccacgat gcgctgcggc
     2401 tacccgggct acgtggccgg cgcgaccttc gcgcaggcgc tgggctggca gggcgccgag
     2461 gtgaacacct cgtacgcggc ctgcgcctcg ggctcgcagg cgctcgccgc cgcgcgcgcg
     2521 aagatcctcg cgggcgaatg cgaggtggcg ctggtcgtcg gcgccgacac cacgccgaag
     2581 ggcttcctgg cgccggccaa gggctaccgt cccgacgatc cggactgggt gcgcttcctg
     2641 gtcggcatca ccaacccgac ctacttcgcg ctctacgcgc gccggcgcat ggacctctac
     2701 ggcgacaccg aggacgactt cggccttgtg aagatcaaga acagcgagca cggattcacc
     2761 aacccgaacg cgcgctatcg caagaagttc acgatggacg acgtgcacgc ctcgccgatg
     2821 gtcgcgcatc cgctgcgcct gctgcagatc tgcgcgacct ccgacggcgg tgcggcactg
     2881 gtggtctcga gcatggacta tgcgcggcgc atcgggcgcg gcgacgcggt gcgcgtcgcg
     2941 gcgatctcga ccgtgacccc gaccttcgcc agcgaagtcg tggagatgcc cgatatcgcg
     3001 accgactcgg ccgccgccgc tgccgtggac acgcaccggt tccgcgcctc gatcccgctg
     3061 aaggcctacg aggaagccgg cgtcgcgcca cgcgacgtga acgtggccga ggtctacgac
     3121 ctgtcctccg cgctcgaact ggactggatg gaagacctgc agctgtgccc gcgcggcgag
     3181 gcggcacgca tggtgcgcgc cggcgagacg cgcatcggcg gccgcgtgcc ggtaaatccc
     3241 tccggcgggc ttgcctgctt cggcgaggcg gtgcccgcgc aggcgctggc gcaggtctgc
     3301 gagctcacgt ggcagctgcg cggccagggc ggcgaacgcc aggtcgaggg cgcgaaggtc
     3361 ggcatcaccg cgaaccaggg cctgttcggc cacggctcct cggtgatcgt caagcgctga
     3421 gccgtccgtg atcgccgcgc catgaagaac gaattgcaca acctgttcgc caacaaccgg
     3481 cgctgggccg ccgcgatccg cgaccgcgat ccggccttct tcgagacgct ggcgcgccag
     3541 cagtcgcctg agtacctgtg gatcggctgc tccgacagcc gcgtgccggc caacgagatc
     3601 atcgggctgt tgcccggcga ggtcttcgtg caccgcaacg tcgccaacgt ggtggtgcac
     3661 accgacctca actgcctctc ggtgctgcag ttcgcggtcg acgtgctgaa catcaagcac
     3721 gtgatggtcg tcggccatta cggctgcggc ggcgtggccg cggcgctgcg caaccgcaag
     3781 ttcggcctcg ccgacaactg gctgcgccac gtgcaggacg tgcagaagaa gcacgccgcc
     3841 accttcgcca acgcgcatcc cgtggacgac gacgcgctgc tggtcgaccg gctgtgcgag
     3901 ctgaacgtga tcgagcaggt gcagaacgtc tgccagacca ccgtggtgca ggacgcctgg
     3961 gagcgcgggc aggatctcac cgtgcacggc tggatctacc gcctgcagga cggggagctg
     4021 cgcgacctgt gcgccaccgc cgccagcccc gccgaggtcg atggcgcgat tcaggccgcg
     4081 atcgcgcggc tgacgccgct cgccccgtga gcggcggatg atccatcttt ccggcgtcgc
     4141 gctcgcgcgc ggcgcgaaaa cactgctcgc cgacgccgaa ctgcgcatcc acgccggcga
     4201 gcgcgtgggg gtgatcgggc agaacggctg cggcaaatcc acgctgttcg cgctgctgcg
     4261 cggcgagatc cagcccgatg ccggcacggc gagcatgccc gccgactggc gcatcgcggc
     4321 gatggagcag gaagtcctcg gcaccgagcg cagggccatc gacttcgtgc tggacggcga
     4381 caccgagttc cgcgcgctcg aggcggcgat ggaaacggcc ggcggcgagc agctcggcga
     4441 gatccacgcg cgcttcgccg ccatcgacgg ctaccgcgcc gcggcgcgcg cgcacgaact
     4501 gctcgacggc ctgggcttcg ccacggccga gcgcgaacgg gcggtgcgcg aattctccgg
     4561 cggctggcgc ctgcggctcg cgctggcgcg cacgctgatg tgcccgagcg acctgatgct
     4621 gctcgacgag cccaccaacc acctcgacct cgacgcgctg gtgtggctgg aagcgtggct
     4681 cgcgcgctat cccggcacgc tgatcctgat ctcgcacgac cgcgatttcc tcgacaacgt
     4741 ctgcaaccgc atcgtcgcct tcgagggcgg cacgctcagc ctgtaccgcg gcaactattc
     4801 ggccttcgaa cgccagcgcg ctgagaagct cgccagccag cagcaggctt tccagaagca
     4861 gcagcagcgc atccaggaga tcgagggctt cgtggcgcgc tttcgcgccc aggccaccaa
     4921 ggcgcgccag gcgcagagcc gcctgaagga actggagcgc atgacgcgca tcgcgcccgc
     4981 gcacatcgac tcgcagttca cgctgcgcct gccctgctac gagcgcgtct ccacgccgct
     5041 gctcaatttc gagcgcgccg cgctggggta cggcgacacg gtcatcctgc caagggtgga
     5101 gctcggcatc atgccggcgc agcgcatcgg cctgctcggc ccgaacggcg ccggcaagtc
     5161 gacgctgatc aagacgatcg ccggcgagct ggcgccgctc gcgggcctgc gccacaccgg
     5221 cgagcacctg cgcatcggct acttcgcgca gcaccagctg gaagcgctgg acctcgcggc
     5281 cacgcccttc ctgcacgtgc agcgcctcag ccccgcggcg agcgagcaat cgatccgcga
     5341 cttcctcggc ggctacggct tccacggcga ccaggcactg ggcaccgtgg gcaatttctc
     5401 gggcggcgag caggcacggc tcgccctcgc gctggtggcc tggcagaaac cgaacctgct
     5461 gctgctcgac gagcccacca accacctcga cctcgagatg cgccacgcgc tgacgctcgc
     5521 gctgcaggaa taccccggcg cggtgctgat cgtctcgcac gaccgccacc tgctgcgcaa
     5581 cacggtcgac gagttcctgc tggtggccga cggccgcgtc gagcccttcg acggcgacct
     5641 cgacgactac cgccagctgc tgctggaaaa gcgcgccgcg ccggcggccg atgtggcggc
     5701 ggaagaaccg ggcgcgcgcg tggaccgccg cgacgagcgc cgcctcgccg ccgagaagcg
     5761 cgagcggctg cggccctggc gcaaccgcat cgcgagcctc gaaaaggaca tggagaaact
     5821 gcaggctgaa ctgcgcgcgc tcgacgaacg gctgcaggat cccgcgctgt acgaggccgc
     5881 ccgcgccggc gaggtgcagg acctgctacg ccgccagggc gcgctgaagc aggagctcgc
     5941 caccgtcgag gaggactggc tcatggcctg cgaggaactc gaatccctgt aggtcgccat
     6001 tcatggcgac aaccgcattc ctgcaggccg tcgtccgcat gaatgcggac ctacgtcgcg
     6061 cggaacgtca gttcctcgcc gctcgcatcc accaccaccg tgctccccgg cgcgaaggcg
     6121 ccggagagga tctgctgcgc cagcggattc tcgatgcggt gctggatcgc gcgcttgagc
     6181 ggccgcgccc cgtagaccgg gtcgaatccg gcgcgcgcca gcaggtcgac ggccgccggc
     6241 gagacatcca gccccaaatc gcgctcgccc aggcgacggc gcagcagctc gagctgcatc
     6301 tgcgcgatgc cgtggatctg ctccgcaccg agcgggtgga acaccaccgt gtcgtcgatg
     6361 cggttgatga actccgggcg gaagtagcgt cccaccacgt ccatcaccgc cgccttcatg
     6421 cgcgggtaat cggcctcggt ggccatttcc tggatcaggt ccgagcccag gttcgaggtc
     6481 atcaccacca cggtgttgcg gaaatccacc gtgcgaccgt gaccgtcggt gagccgcccg
     6541 tcctccagca cctgcagcag cacgttgaac acgtccgggt gggccttctc gacctcgtcg
     6601 agcagcagca gcgagtacgg gcgccggcgc accgcctcgg tgagatagcc gccctcctcg
     6661 tagccgacat agcccggggg cgccccgatc aggcgcgcca cggagtgctt ctccatgaac
     6721 tccgacatgt cgatgcgcac catcgcctcc tggctgtcga acaggaactc cgccagcgcc
     6781 ttgcacaact ccgtcttgcc caccccggtg ggcccgagga acaggaagga gccgtgcgga
     6841 cgcatcgggt ccgacagccc ggcgcggctg cgccgcaccg cgttcgacac cgcgatgatt
     6901 gcctcgtcct gcccgatcac gcgccggtgc agcgcctctt ccatgcgcag cagcttgtcg
     6961 cgctcgccct cgagcatctt cgacaccggg atgccggtcc acttcgacac cacctcggcg
     7021 atctcttcct cgccgacctt gtcgcgcagc agctgcatcc tgtgaccgcc ggactccagc
     7081 tcacccgcag cggcgaggcg cttttccagc gccgggatga tgccgtactg cagttccgcc
     7141 atgcgcgtca ggtcgcccgc gcggcgcgcg ctctccatgt ccaggcgcgc ctgctccagc
     7201 tcgctcttga tctggtgcga gccctgcacg gtggccttct ccgccttcca gatctcgtcg
     7261 agtcccgcgt actcacgctc gagctcggca atctcggcct cgatcttctc caggcgctga
     7321 cgtgccgact ggtcctcgtc cttgcgcacg gcctcgcgct cgatcttcag ctggatcagg
     7381 cggcgatcga ggcgatcgag ctcctcgggc ttggagtcga tctccatgcg gatgcggctc
     7441 gcggcctcgt cgatcaggtc gatggccttg tccggcagct ggcggtcggt gatgtagcgt
     7501 tgcgacaacc tggtcgccgc gatgatcgcc gaatcggtga tcgtcacggc gtggtgcacc
     7561 tcgtagcgct cgcgcaaccc gcgcaggatc gcgatggtgt cctcctcgtt cggctcctcg
     7621 accagcacct tctggaagcg gcgctccagc gcggcgtcct tctcgatgta ctcgcggtac
     7681 tcgttcagcg tggtggcgcc cacgcagtgc agctcgccgc gcgccagcgc gggcttcagc
     7741 atgttgcccg catccagcgc gccctcggcc ttgccggcgc cgaccacggt gtgcagctcg
     7801 tcgatgaaga ggatcacgcg cccttcctcg cgcccgatct ccttcagcac ggctttcagc
     7861 cgctcctcga aatcgccgcg gtacttggcg ccggcgagca gcgcgcccag atccagcgac
     7921 agcagccggc ggttcttcag cccctcgggc acctcgccgt tgatgatgcg ctgcgccagc
     7981 ccctcgacga tcgcggtctt gcccacgccg ggctcgccga tcagcaccgg gttgttcttg
     8041 gttcgccgct gcaacacctg gatggtgcgg cggatctcgt cgtcgcgccc gatcaccggg
     8101 tccagcttgc cctcggcggc acgccgggtg atgtcgatgg tgtatttctc caacgcctgg
     8161 cgcgtaccct cggcctcggg atcgtccacg ccttcgcctc cacgcacctg gtcgatcgat
     8221 tgttccagcg cccggcgctc cagccccgag agcttcagcg cctcgcccac accgctgcgg
     8281 tcctcgagcg ccgcgagcag caccagctcg ctcgagatga acttgtcctt gcgctgctgc
     8341 gcgaacttgt cgctcaggtt cagcagcttg gtgagctcgt tggagacgtg cacctcgccg
     8401 ccgacgccct cgacgcatgg caggcggtcg agcagcgccg tgatgcgctg gcgcagcgcc
     8461 ttgacgtcca cgccgcactg cagcagcagc gggcgcacgg tgccgccctg ctgctcgagc
     8521 agcacgtgca gcaggtgcgc gggcatgatg aagttgtggt cgcgcccgag ggcgagcgat
     8581 tgcgcgtcgg cgatcgcgcc ctggagctga ttggtcaaac ggtccatgcg catggcgggt
     8641 cctctggtgc tggcggggct cactggcgcc ccgtgttgcc acgttatctt ggggcaaccc
     8701 cccgcaattc aagcgtatcc ggaggtgtga atcggccgca ttatccgcgg tctcgcaccg
     8761 gatgcgggcg ggaagcgtac cggtctgacg ctgggcgacc cggtttggct cgtctagaat
     8821 ccgtgctcgc ggcattggcg ccgctgctgc gccacgaaac ccggatcggg agcaacgcgt
     8881 cgatgacatc catggttccg ccgagtcagt tgagcacggc gagcgtcccc cacgcatcgg
     8941 gaaactgcat cggccatttc tacgtcgcca aaagcgtgcg catggcctac ctgtcgatac
     9001 cgaagagcgg ctgcacctcg ctgatcaaca tgctgcatgc ggtcgcgata cccggtttca
     9061 gcacgatcgg cacgcgtatc caccgtgaat ctacgctgcc gcgcgtgcct gcgccggcct
     9121 gccccgcagg gctgctccgc tttaccttcg tgcgcaatcc ctacctgcgg ctgctgtcct
     9181 tttacaggag caaggtcctg aagatggccg accccgaccc gcacgtgaac atgaccctgc
     9241 tgaaaatggg tcttcgggaa ggcatgagct ttgcttcgat ggtcgaagtg ctgtcgacgg
     9301 tgccaccacg ggcgatggac ctgcattgcg ctccgcagat attgttcgtg tatcacatcg
     9361 gcaaacctgt cgtggatttc gtgggcaggc tggagtcgct gcaggaggat tggtccgaac
     9421 tggaggcgcg ctgcggcgtg cacaccgggc tgttgcagga aaacatgaag cacggcatcg
     9481 atctgatcga cgaataccgt gatccctcgg ttcgcgcccg ggttcatgcc ctgtaccgcg
     9541 ccgatttcga gaccttcgga tacgccagtg aactctgagt ccgacgcctt gcgcgcaggc
     9601 ttcggcaacg cgcgcgtgct ggtgagcggc ggcctgggct tcatcggctc gaacctggcc
     9661 ctgcgcctgt gcgaaaccgg cgccgccgtc acgctgctgg acaacctggc gccgagctac
     9721 ggcggcaacc ggttcaacgt cgcgccgatc gcgcggcagg ccgaggtgat catcggcgat
     9781 ctgcgcgacg agcgtctcat gcaccagctc gtcgctggcc aggactacct gttcaacctg
     9841 gccgggcaga ccagccacat cgattcgatg caggatccgc aacaggacct ggaggccaat
     9901 gtccgcgccc agctggcggt tctcgaggcc tgccgcaagg taaacccggg aatccgcgtg
     9961 gtcttcgcca gcacccggca ggtttacggc aagccggcct acctgccggt ggacgaggcg
    10021 catccgctca atcccgtcga tgtcaacggc gtccacaaga tcgccggcga gatgtaccac
    10081 cagctttacg cgcaggttta cggacttgcg accacggtgc tgcgcctgac caacacctac
    10141 ggcccgcgga tgcgggtcag ggatgaccgc cagacgtttc tgggcacctg gatccggcgc
    10201 ctgctcgacg gggaaacgct gcaggtcttc ggcgacggcc ggcaactgcg cgacttcaac
    10261 tacgtggacg acgtggtccg cgccctgatg ctgtgcgcgc tggcccgcga cacggcgggg
    10321 aaggtctaca acctgggatc cagcgaggtg ctgagcctgg aggcgctggc gcagaagctc
    10381 gttgcaacgc acggcagtgg cagctacgag ctggtggcat tcccggacga gcgcaagcgc
    10441 atcgacatcg gggattactt cggcagcagc gcgcgcatcg agcacgatct gggctggcag
    10501 gcacaagtgg gcctcgatgc gggactgcgc cagaccctgg actacttcgg ccgcttcggc
    10561 gggcactact ggccatgacg ataccgattg ccgcacccgt gcgctggttc gagcagcacc
    10621 aacaggcggt ggccgcggcc gtagccggcg tggtgtccgg cggccagtac atcctggggc
    10681 cgcgggtcga tgccttcgaa agcgcattcg cggcgttttg cgcctgcccg cacgcggtgg
    10741 cggtcgcctc gggcaccgac gcgatcacgc tcggccttcg cgtgctcgat gtcgcgggcg
    10801 gagaggtgat caccagcgcc cataccgcgg tggcgacggt tgccgcgatc gagcgcgcgg
    10861 gcgcgacacc cgtggtggcg gatatcgacc ccgtcaccca ttgcctttgc cccgagtcgg
    10921 tagcctccgt gctgtcaccg cgcacgcgcg caatcgtcgc ggtgcacatc ttcggtcacc
    10981 ccgccgatct tccgggcctg cgcgcggtga gcggagcacg ggacatcgga ctcgtcgagg
    11041 attgcgcgca ggcgcacggc gccaggatcg acgggcggcc ggtgggaagc ttcggactgg
    11101 ccggagcctt cagcttctat cccaccaaga acatcggcgc gctgggcgac gccggcgcga
    11161 tcgtgaccca cgacgagcac gtcgcgcggc agttgcgcca gctgcgccaa tacggctggg
    11221 acgagacgcg gtgcagccag ctgcatggcc agaactcgcg catggacgag gtgcaggccg
    11281 cgatcctggc gctgaggctc gcggatttcc ccccggcatt cctgcgacgc cagcagatcg
    11341 cgcagcgcta caacgacgcg ctcgcgaacc atccgcacgt gctggcgccg cagacggcgc
    11401 cgggcgccac gcatgcgttc catctctacg tcgtgcgcac cgcgcagccc gaggcgctcg
    11461 cggcgcacct gctggcgcag ggcatccaga gcgctcgcca ttacgccgcg ccgatccatc
    11521 gccagcccgc ctatcgcgat atcctgcgag cgaagtcact gcgcaacgtg gagcgcctgt
    11581 acgagacgat gctctccatt cccatttacc cggaactcac cgaccgggag gtccacagga
    11641 tatgcgaggc cctgtcaagc tggcgcgcag gagggaaatg aatggtcttc gaaagcaacc
    11701 ggaccatcgt gctctacggc atcggttcgg aatacgtgta cgaggcagtg gagatagcgc
    11761 tgcgcgcggg agtgacggtg gcggcattcg tcgacaacct gccggcatcc gacgggcggc
    11821 agttccccgg gctcgagccc gtactggtgg cgcaggcgtg tgacgagggt gtcagggcgt
    11881 tgccggtcct ggttccgctg atcacgcccg ggcaccgcaa gcaggtcgcg gccgaggcaa
    11941 tgagcctggg tttcgcgggg gtggcaacgc tgatcgaccc caccgcgatc ctggcacgca
    12001 gcacgcgctt cggcgacgga ttccacgtca atgccgcggt cgtcgtcggc gcaaactgcg
    12061 aattcggcag gcaggtactg ctgaaccgat cggcctcgat cggtcacgat gcgcagatcg
    12121 gggacttcgc cagcctgggt cccggctgcg tgctgtgcgg cagctgccgc ataggcgcgg
    12181 gcgccttcat cggcggcggc gccacgatcg cgccgaaggt gagcgtgggc gcgaacgcga
    12241 tcgtcggcgc cggcgccgtg gtgattcgcg acgtgccgcc caactgcttc gtcgcgggca
    12301 atcccgcgcg catcgtgcgc gaaggggtgc agggctacaa cgacgtcggc gtctaggaac
    12361 ggcagtcgtg gaaatcctcg tctattcgca gcagggcaac atgggcggct ccacgcgact
    12421 gctgctcaac ctggcgcgcc acctggctcg cgagcacagt gtcagcgttg ccttcgcaag
    12481 ccaccattcg ccgcaagcca gcgacatcct gctcgcccgc tttccagagc tgcggttgat
    12541 ccccggagat gcgcaaacga tgcgccggcg gcgtttcgat gtggcgattc tccacctgcc
    12601 gttcgcgatg gaggacgtgg acaatttcaa ggcagcgcgc aagatcgcgg tcctgatgga
    12661 gctggtggag cgacacccga tcgcccttcg cgaggaacac tgcgcgcagt tcgagcggat
    12721 tcttcatctg catcgcgagc aggtgcggca tctctcgccc gcctcccggg cagcgaagtg
    12781 cacgcagctg ccggtcatcg acaacatcga cttcgaaccc gaatacgtcc ctgcctgctg
    12841 catcggcgcg atcggcggcg cgcacaagat gcagctgccg ctggccctgt cgctgctgga
    12901 tgcactgccg caacgctact cgatgtgctt ctggtcgccc gacccgctcg ccatcgacgg
    12961 actcgcgctg ccgctgctcg accaggcgct gcggctcgtg tcggccggac gcctgcgaca
    13021 gcaatcgccg agcggcgata tccgcctgct cagccagcgc taccgggcgc tgctgcatac
    13081 gcccctgcac ggcaatggca ccagcgtcgt cgtcagcgac gcgctgagct gtggcaagct
    13141 cgcgatcctg gctccgctgc ccgcctaccg ggaagcctac gggcacctcg atggcgtgct
    13201 gttcaccgac cagcccggga cggaactggc cgggaggatc tgcgcctatt cacgcgagga
    13261 tttttcgcgc atccgcgccg cctatcgggc acaccacgat cgcagtgaca tattgcgcct
    13321 gtggagcgct gcaatcctgg atggcgactt tcacccggcc ctggcccccg gccgcgaacc
    13381 cgagcttgcc accaccgacg ctgccagtgc ggccgctccc cgtgtaatgt gcgccaccgt
    13441 caatccgcat acgagagccc catcgatgtc cgaagccccg tcgcgcaacg ccccatgccc
    13501 ctgcggcagc ggcaaacgct acaagcactg ctgcggggcg gctgcgcagt ccacgcctgt
    13561 cgccgcgcaa gcacccgttc ccgaggtgac gacggagcag ctcaacctgc ggctcgccca
    13621 ttcgatcgaa agctacaatc gcttcgattt ccggctcgct gcgagcctgt gcgacagcgt
    13681 actggcgtcg gacgcggcaa ccgcgctcca gagactcgcg gccgccgaaa tcggcggagc
    13741 cgcctgcacg cgcctggaca ggcacgccag ggcccgggaa ctctacctcg tcgccctgtc
    13801 gattgatccc gatcgcgtcc agacgctgca caatctcggg ctcaacgcga ttcacctcgg
    13861 cgatttcgcg ctggccatcg aatacgcgaa caggctgctg tcggtggaac ccggcaacac
    13921 gaacgcatac aacaccatga ccaacgcgct cagggaactc ggacgctggc acgaagccaa
    13981 ggaactgtac gcccggctca tcgccgacgg acgcgaggac gagaccgtct acaacagcta
    14041 cctgatgtcg ctgcactaca cgccgatgga tccggaggag gcctatcgcg cccacatcgg
    14101 atgggccgag cgctacgcgc cggccgctaa catggcggca agggttcacc acaacgacag
    14161 gtcgacagac cggaaacttc gcctcggcta cttctcgccg aatttcagcc gcaagatcgc
    14221 cggctacttc atcaggccgc tgatcgcaca ccacgatcgc acgcggttcg atctgtatct
    14281 ctattcgaac acggcggtgg aggacgacta caccgactat ttccgcgaca actgcagcaa
    14341 gtggacggat gttcgcgggc tggacgcgaa acaggtcgca caggcgatcg tggacgaccg
    14401 catcgacgtg atggtggatc tcgccgggca tgcgcccgac aacatcctcg ccgcgctgtc
    14461 ttacaagccc gcgccggtcc agatcacgat gctcgattat ttcgacacca ccggcgtgtc
    14521 ggcgatggac tacttcgtct ccgacgacta tcactcgccg gtcggctcgc cacagcgctt
    14581 cgtggagaaa ctgctcaggc tctccaccat ccgtctctgt tacgagccgg tggacctcgc
    14641 gccgccggtg cgcgaactgc ccgcggcgcg caacggatac atcaccttcg gcagtttcaa
    14701 ccgccggcag aaggtcactc ccgaggtgat cggcctgtgg agccgcgtgc tgcatgacgt
    14761 accgggctca cggctggtgc tcaagggaag ctatttcgac caggccgcgg tgcaggccga
    14821 tatcctccac cacttctgtg ccgagggaat cgacgccgcg cgcatcgaat tcagggggcg
    14881 ctcgcaccac cgcgaggcgc tcgagcagta cggcgacgtg gacatcgggc tggacacctt
    14941 cccgtggaac ggtggattga cgacctgcga atccctgctg atgggcgttc ccgtcatcgc
    15001 gctcgcgggc gacaggatca tcagccggca aagcgctgcc atgatgcacg cgctgggtct
    15061 cgatgacttc gtcgccgatg accgggacca gtacgtcgac atcgcccgac gctggtcggc
    15121 cagcaccggt gcgctggagc agctgcgccg cgaactgcgc cagcgcttgc tggcctcgcc
    15181 gctcggagac ggcaagcgtt acgccgccgc ggtggaacag atctacgcga gcacctggcg
    15241 cgcttggtgc gaaggcgctc agccgggcgg acggacgacc tcctgaggcc gcgcgcgatt
    15301 caagcgtcgc cgggggcgcg agaaatccag atcaggcttg ccatgcggcc ggtgaggccg
    15361 tcgcggcggt agctatagaa gagctcgggc gagccgacag tgcagaatcc gccgccgtag
    15421 acacggcgca cgccgagcgc cgcgagcgcg acgcgcgcca gcgcgtagag gtcggcgtgg
    15481 tacctcgacg cgccatcgtg cccgggcagg aatgccgcgc gcaccgcgcc gcgcgcggac
    15541 tcgtcacggc acggggccag gaaggcctcg cgcacctcgg cacccacctc gaaggcgcgc
    15601 tggctgatcg ccgggcccag ccaggcgagc aactccgagc ccggacacgc gaaacgcgcc
    15661 acggtggccg ccagcacgcc ggcggcgagc ccgcgccagc cgcaatgcac cgccgcgacc
    15721 tgcgagcccg cgcggtcggt gagcagcacc ggcaggcaat cggccgtcat caccgcgcac
    15781 accacgccgg gcgcggaggt gtgcgccgcg tcgcccgtgc gcacgatgcc gtcgc
//
LOCUS       DAORUQ010000062        15717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3495_length_15717_cov_6.133125, whole genome shotgun sequence.
ACCESSION   DAORUQ010000062 DAORUQ010000000
VERSION     DAORUQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15717
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3495_length_15717_cov_6.133125"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..296
                     /locus_tag="PLW13_07360"
                     /pseudo
     CDS             1..296
                     /locus_tag="PLW13_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003344757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
     gene            450..710
                     /locus_tag="PLW13_07365"
     CDS             450..710
                     /locus_tag="PLW13_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70228.1"
                     /translation="MSAVQRTGALVRAEARVDAGGATLSVELPHLHHDTALFVPGARL
                     RLRLQLFSVYPDGATARAPATGGDALGVAASRERSRDYGIGT"
     gene            782..2311
                     /locus_tag="PLW13_07370"
     CDS             782..2311
                     /locus_tag="PLW13_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQY70229.1"
                     /translation="MPDANTGLAATGSAHPHARTRPGFMTGRIPTGIRMCSVLVLLFS
                     VSLIPPALVAVQFGEATQVHTFFNAMLLLVGTGVGGLLATRQKSTPLRQRDGFLVVVL
                     FWVVFAACGAVPFVIDSGLHLRFVDAMFESVSGITTTGASVLSGLDAQPRSIIYYRAQ
                     LNFLGGLGIVVLAMALLPYLGVGGAKLYQSETPGPMKEDRMTPRLADTAKSLWLIYSG
                     ITLICALAYHLAGVPWFDAVCYSMSTMSLGGFSTHDASLSLYDSAAVEIVGGVFSLLA
                     AANFALYFRMIQTRSLRPWLVDLEFKFFLKVFGVVAAITIATLWLSETFAPWEATYHG
                     FFQAASVFTGNGINTVGYPGWPAAVVMLLIVSSFFGGCVGSTSGAIKSMRVLLLVREV
                     KREIMQLIHPNAQLSVRLARRSVPEAAVQAVVTFVSLYLISAGVFTLLLCFTGLDPVT
                     AFGTTAGCLNNMGIGYGDTGGGFGGINDAAKWLMSLAMIWGRLEVLPVLVILSRSYWR
                     Y"
     gene            complement(2313..2675)
                     /locus_tag="PLW13_07375"
     CDS             complement(2313..2675)
                     /locus_tag="PLW13_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQY70230.1"
                     /translation="MPERGPAPRRAVLIGALLVALSDAVFLPAMVPAADEAPAAVAAA
                     PRVEIFTTPSCPFCKALRVYLEARGIDYVEHNVNATEETRAAFYASGAPGVPVVMIGE
                     RVIHGFNPVAIEAALKAP"
     gene            complement(2668..3297)
                     /gene="miaE"
                     /locus_tag="PLW13_07380"
     CDS             complement(2668..3297)
                     /gene="miaE"
                     /locus_tag="PLW13_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HQY70231.1"
                     /translation="MAPVLEFLRCPTPDAWIEAALTRQELLLIDHANCEKKAASTALN
                     LMFRYSGDVELLGGLSRLAREELRHFEQVLKLMRERGIAYRRIDAARYAQGLRELVRT
                     HEPARLVDTLIAGAFIEARSCERFARLAPHLDAELAHFYRSLLRSESRHFRDYLAAAR
                     KAADGADIEARIDLFAERERQLIEAQDTQFRFHSGPPAPGASYPEAADA"
     gene            complement(3299..3670)
                     /gene="apaG"
                     /locus_tag="PLW13_07385"
     CDS             complement(3299..3670)
                     /gene="apaG"
                     /locus_tag="PLW13_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQY70232.1"
                     /translation="MNENIDVTVRCEYRADSSEPAASRYAFAYHITISNRSNEHAQLI
                     SRHWIITDGNERRQEVRGLGVVGVQPRIPPGESYQYTSGAMLETAVGTMEGSYRMVRD
                     DGREFSVPIPAFRLAVPHAVN"
     gene            complement(3667..5124)
                     /gene="pyk"
                     /locus_tag="PLW13_07390"
     CDS             complement(3667..5124)
                     /gene="pyk"
                     /locus_tag="PLW13_07390"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY70233.1"
                     /translation="MRKTKIICTIGPATGSYEMLERLYAAGMNVVRLNMSHGDHESHA
                     RVIKAIRTLNRKVRYTVPVMLDTQGPEIRTGHLSAELDLREGQEITIVVRGAEHVEES
                     SIQIDYADIINAVEVGDRITVDNGLINLEVLSKQERVMRCRVVDGGFLKSKRHVNLPG
                     IRVNLPAITEKDRHDIQFGMDQEVDYIALSFVREAKDVRELREFLGEKANKIKIIAKI
                     EDQEGVRNLDEIVQEADGIMVARGDLGVEVNFYELPTIQRRIVRTCAEQGKRVIVATH
                     LLESMIQNPVPTRAEVTDVANAVYEEADAVMLSGETTIGKYPIKCVEYLDRICRAVEP
                     HRGLRFTEKLVLAEQKQSLAASAVHLAELISARGIVVITRRGRMANYVTNCHPHTSVI
                     YAFTNDSRTRRLLALNRGVCAYRSNFSTDPEKTLASAFAILVQREGFRAGDKVVVISD
                     ILEGAGVEAIHVREVPALTAIAAALDTPDDEQDAP"
     gene            5315..5677
                     /locus_tag="PLW13_07395"
     CDS             5315..5677
                     /locus_tag="PLW13_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70234.1"
                     /translation="MTDFISFSRVEHELAPKLRNSLNHAESSEDAKKFFSYTAQELLG
                     KALGEAVKVHYEDVALSRDGQGFTVSARLQADAGYAKVAAGSDLKAILERFAESAQHR
                     VRHLESHPEKTQSKIRGH"
     gene            5698..6408
                     /locus_tag="PLW13_07400"
     CDS             5698..6408
                     /locus_tag="PLW13_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY70235.1"
                     /translation="MHEQYIEYSHAGTLLEGFYVNPARAGEKRPAVLISHAWAGRDEF
                     VIEKAKALAGLGYAAFALDNYGKGVLGSSTEENSGLMGPFLADRALLRDRLQAGLATL
                     RAQPGVDADRIAIMGYCFGGLCALDLARSGADIRGAISFHGLFLPSGLEPRPITAKVL
                     ALHGYADPMVPPEQVVSFGKEMTDAGVDWQLHAYGGVLHAFTVPEANDLSLGALYNAD
                     AERRSWRALVDFLAEIFA"
     gene            complement(6513..7877)
                     /gene="radA"
                     /locus_tag="PLW13_07405"
     CDS             complement(6513..7877)
                     /gene="radA"
                     /locus_tag="PLW13_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQY70236.1"
                     /translation="MAKSRVVYVCQDCGSEQNKWAGQCPDCGAWNTLSQFVEAGAARG
                     IGGARRGGYAGAVAELQTLDQIDLAEVPRFSTGYSEFDRVLGGGVVPGSVNLVGGQPG
                     AGKSTLLLQVMCALSQQMETLYVTGEESLQQVALRAHRLGLPTDKLKMLAETSVESIL
                     ATAAKLSPRVMVIDSVQVVHCEDVPSAPGGVAQVRESAAQFTRFAKAGGAAMFLVGHV
                     TKDGTLAGPKVLEHIIDCSIMLEEEGDSRFRSLRAGKNRFGAVNELGVFAMTDRGMRE
                     VRNPSAIFLSRGADISPGSVVMVVWEGTRPLLVEIQALVDESMGSNPRRVAVGVDQNR
                     LSMLLAVLHRHGGLQLGDQDVFANVVGGIKVAETSADLALLLAIVSSYRDRSIDAGLV
                     SFGEVGLSGEIRPVPSGGERLLEAAKHGFRRAIVPAANVPRKPVEGMDVVGVSSLAQA
                     LDAL"
     gene            7992..9098
                     /locus_tag="PLW13_07410"
     CDS             7992..9098
                     /locus_tag="PLW13_07410"
                     /inference="COORDINATES: protein motif:HMM:NF037684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HQY70237.1"
                     /translation="MASSLIHERTLLISPTLAATIGLEEAVMLQLLHDAAQFSARPGA
                     RWHELDKVELLRLLPFWSERDCQRISASLRDKGVLAIDSPPLEQTHCLRFALEEPAAA
                     PAARAPRAPEHRPGGGKALIATRWQPDEEVLRQIALHGIPRVFAEAQLDEFVLYWSDR
                     GEPAFSWNAKFRAHVIRRWREQEASTGVQEDVPRPIGADWEPGPDAMEILVRAGVSRH
                     FVEDAVPEFVLYWTERREASRTWNSRFVTHVKRQWARYTQTMDLDSEPRRIPANWEPA
                     ADVYDILRMANIDAAFARALLPEFLMFWIDSGAVYPSWNSKFLQHVKYHWARSHRAGD
                     TAEQTHGTRQRSGGTGNTRSRSLAEDLTDRSWAD"
     gene            9010..9681
                     /locus_tag="PLW13_07415"
     CDS             9010..9681
                     /locus_tag="PLW13_07415"
                     /inference="COORDINATES: protein motif:HMM:NF018671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HQY70238.1"
                     /translation="MEHGKDLVERAIRDLEASPRTSRTGAGPTEARRDPALVDAINQV
                     FALFRLNYHNQYYSAFGDAGQLSQVKRLWLDSLAGHGTETILLGARRCIETSEYLPTL
                     HRMLECCREATLQAQGLPDAHAAYREACLAPEPKQAARWSHPAVFHAGREAGWFFLAS
                     TPEPKAFPVFEKRYRAVCDRIVAGEVLQLPAPEHGPQAAAALVAPDADEQRARLATLR
                     AETGV"
     gene            complement(9693..10205)
                     /locus_tag="PLW13_07420"
     CDS             complement(9693..10205)
                     /locus_tag="PLW13_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQY70239.1"
                     /translation="MLEHALETGRIGVCVKDRDRRVLTQNASCREICGDRQGEICEDG
                     CMRLHEADAGRQWQDRGCSAYRNSAMHGGSYDVALVCSAERIITFLQPLEDVYASAIA
                     HYADKGLTRREHEIVGLLIRGRSNAELCAGLGITRATLRTHLNRIYAKLRDSGAAVDF
                     LPRHRLAAKR"
     gene            complement(10253..12019)
                     /locus_tag="PLW13_07425"
     CDS             complement(10253..12019)
                     /locus_tag="PLW13_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY70240.1"
                     /translation="MRLMAEVLRRYPRQSVVMLLALLCAGLAEGLSLGSLAPMLGVAV
                     GGGAGGGTALADAGTLPPALLHWLDGLDFAVRIGTLLGIIVGGLTLKNLLLLCANRQV
                     GLASARFATDLRLGLLGAVLRGRWDYFVHQPVGRLSNALATEVQRSATAVVDGSRALT
                     FLVQALIYAGVALAVSWRATLVCVGVGVLVLGLTSLLVRAARRSGSNQTLRLRALVTR
                     LTDALQSVKALKAMSREHLVVSVLSAETTRLDAALRAQVASTAALAAGQEQMFAMAIG
                     LGMFVALVYVDMPFATVLVLVLVLGRMLSQCGKVQKEYQRLVIGESAYVAIVEALAEA
                     RRAEEAPGGTREPTLERGIRIEALHFARGARELFEGITLEIRAGSLTTLTGASGCGKT
                     TLVDMIIGLVHPCSGRVTVDDVALGELDLRAWRHMIGYVPQENLLLHDTILGNVTLGD
                     PALGAADAERALREAGAWEFVAALPEGMQHVVGERGATLSGGQRQRIMIARALVNRPR
                     LLVLDEATSALDAGAEAAICATLARLRGRLTIVAITHREAMARIADRVYRLDAMVHAA
                     REGGAVQLLRPANAPYSLAASS"
     gene            complement(12010..12969)
                     /locus_tag="PLW13_07430"
     CDS             complement(12010..12969)
                     /locus_tag="PLW13_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70241.1"
                     /translation="MFSSEARIRDSRWREPVRRIEAAALGLFWLGARALPPRRAARTG
                     ARLFAWLGPRLRKQAFVRSNLARLFPAAGPARIEALAREAWANFGAILAEYPHLPRFC
                     APDPSPFLRVAIAPETRDIVSRREPAIYVAAHLANWELATAAIGRLGIALSAVYAPQG
                     NPYVDAMIQARRAPLGCRFLGKANCMRRLLSDLEAGRSVGMLPDQRTDSGELVPFFGG
                     LAPTTIGPAWLALRRGCPLVPVQVERLGPADYQVTFHAPLVRGERSADRSRALRLTAE
                     LNALFERWIRVHPGQWLCMRRRWPPAAAPGQLQAVGGEVRACA"
     gene            13112..14245
                     /locus_tag="PLW13_07435"
     CDS             13112..14245
                     /locus_tag="PLW13_07435"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HQY70242.1"
                     /translation="MFDLHAGTTHRGNHHPRVWLLNGPRPGDNNQVLGLGETLGWPFE
                     LKSVGFHSSPPVPKMISSQLRSLALAGVDRRRSSALQAPWPDLVISAGRELEPVARWI
                     KKRSGNRARLVHMGRPWSRLDAFDLIISTPQYFLPERENILLNELPLHRVTERRLEEA
                     RALWAPRFAHLPRPYTVVLLGGNSGSFVLSCAKARRLGKLVNALTRGSGGSLLVTSSA
                     RTPADAYHDFLDGIGGPRHEHHWGAQENPYFGYLALADRIVITGESVSMLAEANVTGK
                     PLLIYDIAERPAPDMPPWQRLAIRLSYRSLRDLFARTIGAPHMQRDIGAIQRHLVSIG
                     RAAWLDEASFGSAASQQPWHAPRLDAQRAASRVQNLLAGVLPC"
     gene            14239..14904
                     /locus_tag="PLW13_07440"
     CDS             14239..14904
                     /locus_tag="PLW13_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70243.1"
                     /translation="MLTARALMTLLRAMMGVAANLLLAVLLHWRETESLYQENNLLEN
                     LQLAALAGCVLVYGLRARRGVDGYARLQLGALALLSACMFYRESDFRWLLPEGTLVYA
                     IAYVVHRGAFVLALLGVAGALARCWLLDRRRFLDFLDSGPGMLLVLATVLLAGGVLLD
                     KSVISIAAPNRLFEELFELNAYGLMLIAGGSRALGRPPARHAPGSSGVPAAGPARTVP
                     LDR"
     gene            14901..>15717
                     /locus_tag="PLW13_07445"
     CDS             14901..>15717
                     /locus_tag="PLW13_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HQY70244.1"
                     /translation="MNVPPCACALPSESCPRFSPATRAGALARIGLISNANSGCNRLH
                     LAGVHRILSRHAGLRHVVTGSADEVRHAVAELAREHIDVLAINGGDGTAASVLGHVLA
                     QAPFATHPRVVLLPGGTVNMAATAIGTGGDLLRAATRLSGWISERPRPCREVRQSILR
                     VQLGDHGAAHHGMFLGAGAVIKGTEYAHRRVHAMGLRGSESLGLALTRTLWGLQRRDP
                     RFAHAVPVTLTLDDAPPLPQQDALILLVSSLQRLSLGMCPFWGSENGAVRLTLI"
BASE COUNT         2322 a         5629 c         5412 g         2354 t
ORIGIN      
        1 tggcgctggc gcgcgcgctg gccatcgagc cgcgcgtgct gctgctcgac gagcccttcg
       61 gcgcgctcga cgccaaggtg cgcaaggatc tgcggcgctg gctgcgcgag ctgcaccagc
      121 gcatcggcac caccacgata ttcgtgaccc acgaccagga ggaggcgctc gagctggccg
      181 accgcgtggt gatcatgaac cagggtcgca tcgagcaggc cggcagcgtg aacgaggtct
      241 acgagtaccc cgcctcgccc ttcgtgttcg agttcctcgg caacaccaat atcctgccgg
      301 ccacggccga gggacgcgcg ctgcacatcg ccggcgccga ccgggcgctg cagacctcgc
      361 agatccaccc ggccggcccg ctggcggtct acgtgcggcc ggcggacctg cgcgtggccg
      421 aggagggcgg gcacggcatc ggcgtcacgg tgagcgcggt gcagcgcacc ggtgccctgg
      481 tgcgcgccga ggcgcgcgtg gacgccggcg gcgcgacgct gtcggtggaa ctgccgcacc
      541 tgcaccacga caccgcgctc ttcgtgcccg gcgcgcggct gcgcctgagg ctgcaactgt
      601 tcagcgtgta tcccgacggc gcgacggccc gggcgccggc caccggcggc gatgcgctgg
      661 gggtggcggc gtcgcgcgag cgcagccgcg actacggcat cgggacataa ccccgcgggc
      721 gtattgatcg gcactgcgcg cggccacaga atgcgcgcgt caacccgatc gcaggtgccc
      781 gatgcccgac gcgaataccg ggctcgccgc caccggcagc gcccaccccc atgcccgcac
      841 acggcccgga ttcatgaccg gacgcatccc gaccggcatc cgcatgtgca gcgtgctggt
      901 gttgctgttc agcgtctcgt tgatcccgcc ggcactggtg gccgtgcagt tcggcgaagc
      961 cacgcaggtg cacaccttct tcaacgccat gctgctgctg gtcggcacgg gggtaggcgg
     1021 cctgctggca acgcgccaga aatccacacc gctgcgccag cgcgacggtt tcctcgtggt
     1081 ggtgctgttc tgggtggtat tcgccgcctg cggcgcggtg cccttcgtca tcgacagcgg
     1141 cctgcacctg cgcttcgtcg acgcgatgtt cgaatccgtc tccggcatca ccaccaccgg
     1201 cgcctcggtg ctgagcggcc tcgatgcgca gccgcgcagc atcatctact accgcgcgca
     1261 gctgaacttc ctcggcggcc tgggcatcgt ggtgctcgcc atggcgctgc tgccctacct
     1321 cggggtgggc ggcgccaagc tgtaccagtc cgaaacgccg ggcccgatga aggaagaccg
     1381 catgacgccg cggctcgccg acaccgccaa gagcctgtgg ctgatctact cgggcatcac
     1441 gctgatctgc gcgctggcct accacctggc gggcgtgccc tggttcgacg cggtgtgcta
     1501 cagcatgtca accatgtcgc tgggcggctt ctcgacccac gacgccagtc tcagcctgta
     1561 cgacagcgcc gcggtggaga tcgtcggcgg cgtgttctcg ctgctcgcgg cggccaactt
     1621 cgcgctgtac tttcgcatga tccagacacg cagcctgcgg ccgtggctgg tcgatctgga
     1681 gttcaagttc tttctgaaag tgttcggcgt cgtcgccgcg ataacgatcg cgaccttgtg
     1741 gctgtccgaa accttcgcgc cgtgggaagc cacctatcac ggcttcttcc aggccgcctc
     1801 ggtgttcacc ggcaacggca tcaacaccgt cggctacccc ggctggccgg ccgccgtggt
     1861 gatgctgctg atcgtgtcga gtttcttcgg cggctgcgtg ggctcgacct cgggcgcgat
     1921 caagtcgatg cgcgtcctgc tgctggtgcg cgaggtcaag cgcgagatca tgcagctcat
     1981 ccaccccaac gcgcagctct cggtgcgcct ggcgcggcgc agcgtacccg aggcggcggt
     2041 gcaggcggtg gtcacctttg tcagcctcta cctgatcagc gccggcgtct tcacgctgct
     2101 gctctgtttc acggggctcg atcccgtcac cgcattcggc accaccgcgg gctgcctcaa
     2161 caacatgggc atcggctacg gcgacaccgg cggcggtttc ggcggcatca acgatgcggc
     2221 caagtggctc atgtcgcttg ccatgatctg ggggcgcctc gaggtgctgc cggtgctggt
     2281 gatcctgtcg cgcagctact ggcgctattg acctacggtg ctttcagggc ggcttcgatg
     2341 gcaaccggat tgaagccgtg gatcacgcgc tcgccgatca tgaccacggg cacgcccggc
     2401 gcgccgctgg cgtagaaggc cgcgcgcgtc tcctcggtgg cattcacgtt gtgctcgacg
     2461 taatcgatgc cacgcgcctc gaggtagacg cgcagcgcct tgcaaaaggg gcaggagggc
     2521 gtggtgaaga tctccacgcg cggcgcggcg gcaacggctg ccggggcctc gtcggccgcc
     2581 ggcaccatgg cgggcaggaa gacggcatcg gacagcgcga caagcagcgc accgatgagc
     2641 acggcgcggc ggggagcagg cccgcgctca ggcatcggcc gcctccgggt acgacgcgcc
     2701 cggcgcgggc ggaccgctgt gaaaacgaaa ctgcgtgtcc tgcgcctcga tcagctgacg
     2761 ctcgcgttcg gcgaacagat cgatgcgcgc ctcgatgtcg gcgccatcgg cggccttgcg
     2821 cgccgcggcg aggtaatcgc ggaagtggcg cgactccgag cgcagcagcg agcggtagaa
     2881 gtgcgcgagc tccgcgtcga gatgcggcgc cagccgcgcg aagcgctcgc aggagcgggc
     2941 ctcgatgaac gccccggcga tcagcgtgtc cacgagccgc gccggctcgt gggtgcgcac
     3001 cagctcgcgc aggccctgcg cataacgcgc cgcgtcgatg cgacggtacg cgatgccacg
     3061 ctcgcgcatc agcttcagca cctgctcgaa atgcctgagc tcctcgcgcg ccaggcgcga
     3121 caggccgccg agcaattcca cgtcgccgga atagcggaac atcaggttga gcgcggtgct
     3181 cgcggctttc ttctcgcagt tggcgtggtc gatcagcagc agttcctggc gcgtgagcgc
     3241 ggcctcgatc caggcgtccg gggtgggaca gcgcaggaat tccagcaccg gtgcgatatc
     3301 agttgaccgc atgcggtacc gcgaggcgaa aggcgggaat gggcacgctg aactcgcgtc
     3361 cgtcgtcgcg caccatgcgg tagctgccct ccatggtgcc gacggcggtc tcgagcatcg
     3421 cgccgctggt gtactggtag gattcgcccg gcgggatgcg tggctgcact ccgaccaccc
     3481 cgaggccgcg cacctcctgg cggcgctcgt tgccgtcggt gatgatccag tgccggctga
     3541 tcagctgcgc gtgctcgttg ctgcgattgc tgatcgtgat gtggtaggca aaggcgtagc
     3601 gcgaggccgc gggctcggag ctgtcggcgc ggtattcgca gcgcaccgtg acatcgatgt
     3661 tctcgttcat ggcgcgtcct gctcgtcgtc cggggtgtcg agggcggccg cgatggcggt
     3721 gagtgcgggc acctcgcgca cgtggatggc ctcgacgccg gcgccctcga ggatgtcgga
     3781 gatcaccacg accttgtcgc cggcgcggaa tccctcgcgc tgcaccagga tcgcgaaggc
     3841 gctggcgagc gtcttctcgg gatcggtgct gaagttgctg cgataggcgc agacaccgcg
     3901 gttcagcgcg agcaggcggc gcgtgcgcga gtcgttggtg aacgcgtaga tcaccgaggt
     3961 gtgcggatga cagttggtca cgtaattggc catgcgcccg cgccgcgtga tcaccacgat
     4021 accccgcgcg gaaatgagct ccgcgagatg caccgcgctc gccgccaggc tctgcttctg
     4081 ctcggcgagc acgagcttct cggtgaagcg cagcccgcgg tgcggctcca ccgcgcggca
     4141 gatgcggtcg aggtactcga cgcacttgat cgggtacttg ccgatcgtgg tctcgcccga
     4201 gagcatcacc gcgtcggctt cctcgtagac ggcgttggcg acgtcggtga cctcggcgcg
     4261 cgtgggcacc gggttctgga tcatggattc gagcaggtgg gtggcgacga tcacgcgctt
     4321 gccctgctcg gcgcaggtgc gcacgatgcg ccgctggatg gtcggcagct cgtagaagtt
     4381 cacctccacg ccgaggtcgc cgcgcgcgac catgatgccg tcggcctcct gcacgatctc
     4441 gtcgaggttg cgcacgccct cctggtcctc gatcttggcg atgatcttga tcttgttcgc
     4501 cttctcgccg aggaactcgc gcagctcgcg cacgtccttg gcctcgcgca cgaaggacag
     4561 cgcgatgtag tccacctcct ggtccatgcc gaactggatg tcgtggcggt ccttctcggt
     4621 gatcgcgggc aggttcaccc ggatgccggg caggttcacg tggcgcttgc tcttcaggaa
     4681 gccgccgtcg accacgcggc agcgcatcac gcgctcctgc ttgctcagca cctcgaggtt
     4741 gatcaggccg ttgtcgaccg tgatcctgtc gcccacctcg acggcgttga tgatgtcggc
     4801 gtagtcgatc tggatcgagc tctcctcgac gtgctcggcg ccgcgcacca cgatggtgat
     4861 ctcctggccc tcgcgcaggt ccagttccgc cgagaggtgc ccggtgcgga tctccgggcc
     4921 ctgggtgtcc agcatcaccg gcacggtgta gcgcaccttg cggttcagcg tgcgtatggc
     4981 cttgatcacg cgcgcgtgcg actcgtggtc gccgtgcgac atgttgagcc gcacgacgtt
     5041 catgccggcg gcgtagagcc gctcgagcat ctcgtaggag ccggttgcgg ggcctatggt
     5101 gcagatgatc ttggtcttgc gcatcggatg tctggtgggt gcctggagcg ggccatcata
     5161 gcagcaatcc gggcgcctcc cgactacccg ccgggccggc tgcgggcagc tgcaaggccc
     5221 tttgcgcgcg ggtttgccgc acgccggcgg ccgagggtag tatgccaagc cacgccccgt
     5281 ccggggcgcc acaccgccgg ggagtaacca ttcgatgaca gacttcatct cgttttcgcg
     5341 cgtagagcac gagcttgcgc ccaagctgcg caacagcctg aaccacgccg agtcgtcgga
     5401 ggacgcgaag aagtttttca gttacacggc ccaggaactg ctcggcaagg cgctcggcga
     5461 ggccgtgaag gtgcactacg aggacgtggc gctgtcgcgc gacgggcagg gtttcacggt
     5521 cagcgcacgc ctgcaggccg atgccggcta cgcgaaggtc gccgcgggct cggacctgaa
     5581 ggcgatcctg gagcgcttcg ccgagtcggc ccagcaccgc gtgcgccacc tcgagagcca
     5641 tcccgagaag acccagtcca agatccgcgg ccattgatcg gcacaggagc acccgcgatg
     5701 cacgagcaat acatcgaata cagccatgcc ggcacgctgc tggaaggttt ctacgtcaac
     5761 cccgccaggg ccggcgagaa gcgccccgcg gtgctcatct cgcacgcctg ggccgggcgc
     5821 gacgagttcg tgatcgagaa ggcgaaggcg ctggcgggtc tcggctacgc ggccttcgcg
     5881 ctcgacaact acgggaaggg ggtgctgggc tccagcacgg aggagaattc ggggctgatg
     5941 gggccgttcc tcgccgaccg cgcgctgctg cgcgaccgcc tgcaggcagg gctggcgacg
     6001 ctgcgcgcgc agcccggcgt cgatgcggac cgcatcgcga tcatgggcta ctgcttcggc
     6061 ggcctgtgcg ccctggatct cgcgcgcagc ggcgccgata tccgcggcgc catctccttc
     6121 cacggcctgt tcctgccgag cgggctggag cccaggccga tcacggcgaa ggtgctggcg
     6181 ttgcacggct acgccgaccc gatggtgccg cccgagcagg tggtgtcctt cggcaaggag
     6241 atgaccgacg ccggcgtcga ctggcagctg cacgcctacg gcggcgttct gcacgccttc
     6301 acggtgccgg aggcgaacga cctcagcctc ggcgcgctct acaacgccga cgccgagcgc
     6361 cgctcgtggc gggcgctggt ggatttcctg gcggagatct tcgcctgata cgcatgaggt
     6421 cgcgggcatg gcccgctcct acaagtgttc gggcgcatgc cggattcccg taggagcggg
     6481 ccatgcccgc gacctgattc cccggcccac gatcacaacg catccagcgc ctgcgcgagg
     6541 ctgctgacgc ccacgacatc catgccctcg acgggtttgc gcggcacgtt ggccgccggc
     6601 acgatggcgc ggcgaaagcc gtgcttggcg gcctccagca ggcgctcgcc accgctgggc
     6661 acggggcgga tctcgcccga caatcccacc tcgccgaagc tgaccagtcc cgcgtcgatg
     6721 ctgcggtcgc ggtagctgga aactatggcc aacagcagcg cgaggtcggc gctggtctcg
     6781 gccaccttga tgccgcccac cacgttggcg aacacgtcct ggtcaccgag ctgcaggccg
     6841 ccgtggcggt gcagcaccgc gagcagcatc gacagccggt tctggtccac gcccaccgcc
     6901 acgcggcgcg ggttgctgcc catgctctcg tcgaccagcg cctggatctc caccagcagc
     6961 ggccgcgtgc cctcccacac caccatcacc acgctgcccg gcgagatgtc ggcaccgcgc
     7021 gagaggaata tcgccgaggg gttgcgcacc tcgcgcatgc cgcggtcggt catcgcgaac
     7081 acgccgagct cgttcaccgc gccgaagcgg ttcttgcccg cgcgcagcga tcggaaacgg
     7141 ctgtcgccct cctcctcgag catgatcgag cagtcgatga tgtgctccag taccttcggg
     7201 cccgcgagcg tgccgtcctt ggtgacgtga ccgaccagga acatcgccgc cccgccggcc
     7261 ttggcgaagc gcgtgaactg cgccgcgctc tcgcgcacct gcgccacgcc gccgggcgcc
     7321 gagggcacgt cctcgcagtg caccacctgc accgagtcga tcaccatcac gcgcggcgac
     7381 agcttcgccg ccgtggccag gatggattcc acgctggtct cggcgagcat cttcagcttg
     7441 tcggtgggca gaccgagtcg gtgcgcgcgc agcgccacct gctgcagcga ctcctcgccg
     7501 gtgacgtaga gcgtctccat ctgctgcgac agcgcgcaca tcacctgcag cagcagcgtg
     7561 ctcttgcccg cgccgggctg gccgccgacc aggttcaccg agcccggcac cacgccgccg
     7621 cccagcacgc ggtcgaactc gctgtagccg gtggaaaagc gcggcacttc cgccaggtcg
     7681 atctggtcca gcgtctgcag ctcggccacc gcgccggcat aaccgccgcg gcgcgcgccg
     7741 ccgatgccgc gcgcggcccc cgcctcgacg aactggctga gcgtgttcca ggccccgcaa
     7801 tcggggcact ggcccgccca tttgttctgc tcggaaccgc aatcctggca gacgtaaacc
     7861 actctggact ttgccacgcc cgatccttac gcttggcgac cgttcaacct cagcgcccga
     7921 ttgtggcagg gccgacgccc gcacaccaag tcccgggcgc gccgcgccgg cgcagatccc
     7981 gaatcccgtc catggcatcg agtctcatcc acgaacgcac gctgctcatc tccccgacgc
     8041 tggccgccac catcggcctc gaagaggccg tgatgctgca gttgctgcac gacgccgcgc
     8101 agttcagcgc gcgcccgggt gcgcgctggc acgagctcga caaggtcgag ctgctgcggc
     8161 tgctgccgtt ctggagcgag cgcgactgcc agcgcatcag cgccagcctg cgcgacaagg
     8221 gcgtgctggc gatcgattcg ccgccgctgg agcagacgca ctgcctgcgc ttcgcgctgg
     8281 aggagcccgc cgccgcgccg gccgcgcgcg cgccgcgggc ccccgagcac cggcccggcg
     8341 gcggcaaggc gctgatcgcc acgcgctggc aacccgacga ggaggtgctg cgccagatcg
     8401 cgctgcacgg catcccgcgc gtcttcgccg aggcgcagct cgacgagttc gtgctgtact
     8461 ggtccgatcg cggcgaaccg gccttctcgt ggaacgcgaa gttccgcgct cacgtgatcc
     8521 ggcgctggcg cgagcaggag gcgagcaccg gggtgcagga ggacgtgccg cgcccgatcg
     8581 gcgccgactg ggagccgggg cccgacgcga tggaaatcct cgtgcgcgcc ggcgtcagcc
     8641 gccacttcgt cgaggacgcg gtgccggagt tcgtgctgta ctggaccgaa cggcgcgagg
     8701 cctcgcgcac ctggaactcg cgcttcgtga cgcacgtcaa gcgccagtgg gcgcgctaca
     8761 cccagaccat ggatctcgac agcgagcccc ggcgcatacc ggccaactgg gagccggcgg
     8821 cggacgtcta cgacatcctg cgcatggcca acatcgacgc tgccttcgcg cgcgcactgc
     8881 tgccggaatt cctgatgttc tggatcgatt cgggcgcggt ctacccctcg tggaacagca
     8941 agttcctgca gcacgtgaag tatcattggg cgcgcagcca ccgagcgggc gacaccgcgg
     9001 agcagactca tggaacacgg caaagatctg gtggaacggg caatacgcga tctcgaagcc
     9061 tcgccgagga cctcacggac cggagctggg ccgactgagg cgcgccgcga cccggcgctg
     9121 gtcgacgcca tcaaccaggt cttcgcgctg tttcgcctga actaccacaa ccagtactac
     9181 agcgccttcg gcgacgccgg gcagctgagc caggtcaagc ggttgtggct cgacagcctg
     9241 gccggacacg gcaccgagac catcctgctc ggcgcgcgcc gatgcatcga gacctcggaa
     9301 tacctgccga ccctgcaccg catgctcgag tgctgccgcg aggcgacgct gcaggcgcag
     9361 ggcctgcccg atgcgcatgc ggcctatcgc gaggcctgcc tggcgcccga acccaagcag
     9421 gcggcgcgct ggagccaccc ggcggtattc catgccgggc gcgaggccgg ctggttcttc
     9481 ctggcatcga cgccggagcc caaggccttc ccggtgttcg agaagcgcta ccgcgcggtg
     9541 tgcgatcgca tcgtggcggg cgaggtgctg cagctgccgg cgcccgaaca cggcccccag
     9601 gcggcagcgg ccctcgtcgc ccccgacgcc gacgagcagc gcgcgcgcct cgcaacgctg
     9661 cgcgccgaaa cgggcgtgta ggtccggccg cttcagcgct tggcggcgag gcggtggcgc
     9721 ggcaggaaat cgaccgccgc gccgctgtcg cgcagcttgg cgtagatcct gttcaggtgc
     9781 gtgcgcagcg tggcgcgcgt gatcccgagc ccggcacaga gttcggcatt gctgcggccg
     9841 cggatcagca aaccgactat ctcgtgctcg cgtcgcgtga ggcccttgtc ggcgtaatgc
     9901 gcgatcgcgg aggcataaac gtcctccagc ggctgcagga acgtgatgat gcgctcggcg
     9961 ctgcacacca gcgccacgtc gtacgagccg ccgtgcatcg cactgttgcg gtaggcgctg
    10021 catccccggt cctgccactg ccggccggcg tcggcctcgt gcaggcgcat gcagccgtcc
    10081 tcgcagatct cgccctggcg gtcgccgcag atttcgcgac agctcgcgtt ctgcgtcagc
    10141 acgcgcctgt cgcggtcctt cacgcagacc ccgatgcgtc ccgtctccag cgcgtgctcg
    10201 agcatggcct gacccgcatt cgcccatgaa accgccgcca tttagccgcc gctcaggacg
    10261 aggcggcaag ggaatatggc gcatttgcgg gccgcagcaa ttgcacggcg ccgccctcgc
    10321 gcgcggcgtg caccatggcg tcgaggcggt agacgcgatc ggcgatccgc gccatcgcct
    10381 cgcgatgcgt gatcgcgaca atcgtcaacc tcccgcgcag gcgcgccagc gtggcgcaga
    10441 tagccgcctc ggccccggcg tcgagcgcgc tggtcgcctc gtccagcacg agcaggcgcg
    10501 gacggtttac aagggcgcgg gcgatcatga tgcgctggcg ctggccgccg gagagcgtgg
    10561 ccccgcgctc gcccacgaca tgctgcatgc cctcgggcag cgccgccacg aattcccagg
    10621 cgccggcctc gcgcagtgcg cgctcggcat cggcggcgcc gagtgcggga tcgccgagcg
    10681 tgacgttgcc gaggatcgtg tcgtgcagca gcaggttctc ctgcggcacg tagccgatca
    10741 tgtgccgcca cgcgcgcagg tccagctccc cgagcgccac gtcatcgacc gtgacgcgcc
    10801 ccgagcacgg atgcaccagg ccgatgatca tgtccaccag cgtggtcttg ccgcaaccgg
    10861 aagctccggt gagcgtcgtg agcgagccgg cgcggatctc gagcgtgatg ccctcgaaga
    10921 gttcgcgcgc gccgcgcgcg aagtgcagcg cctcgatgcg gatgccgcgc tccagcgtcg
    10981 gctcgcgcgt cccgcccggg gcctcctcgg cacggcgcgc ctccgcgagc gcctcgacga
    11041 tggccacgta ggcgctctcg ccgatgacca ggcgctggta ctccttctgc accttcccgc
    11101 actgcgacag catgcgcccc agcaccagca ccagcaccag caccgtggcg aagggcatgt
    11161 cgacgtacac gagcgccacg aacatgccca gcccgatcgc catggcgaac atctgctcct
    11221 gccccgcggc gagcgccgcc gtgctcgcga cctgggcgcg cagggccgcg tcgagacgcg
    11281 tcgtctccgc cgacagcacc gacacgacga ggtgctcgcg cgacatcgcc ttcaacgcct
    11341 tcacggactg caacgcatcg gtgagccggg tcaccagcgc gcgcaggcgc agcgtctggt
    11401 tgctccccga gcggcgagcg gcgcgcacga gcaggctggt gaggccgagc accagcacgc
    11461 ccacgcccac gcacaccagc gtcgcgcgcc acgataccgc cagcgcgacc ccggcataga
    11521 tcagcgcctg gaccaggaag gtcagcgcgc gggaaccgtc gacgacggcc gtcgccgagc
    11581 gctgcacctc cgtcgccagc gcgttgctca gccgcccgac cggctggtgc acgaagtagt
    11641 cccagcgccc ccgcagcacc gcgccgagca gcccgagacg caggtccgtg gcgaaacgcg
    11701 ccgaagccag ccccacctgg cggttggcgc acagcaacag caggttcttg agcgtcagtc
    11761 cgcccacgat gatgccgagc agggtgccga tgcgcaccgc gaagtccagc ccgtcgagcc
    11821 agtgcaggag cgcgggcggg agcgtgcccg catccgcgag cgcggtaccg ccgcccgcgc
    11881 cgcctccgac cgccacgccg agcatcggcg ccaggctgcc caggctcagc ccctcggcga
    11941 gacccgcgca gagcagcgcg agcagcatga cgacgctctg ccgggggtag cggcgcaaca
    12001 cctccgccat caggcgcatg cgcggacctc cccgccgacc gcctgcagct gccccggcgc
    12061 tgccgcgggc ggccagcgcc ggcgcatgca cagccactgc ccggggtgca cccggatcca
    12121 gcgctcgaac agcgcattga gttccgcggt gaggcgcagc gcgcgcgagc ggtccgccga
    12181 gcgctctccg cgcacgagcg gcgcgtggaa cgtcacctgg tagtcggccg gtccgagccg
    12241 ctcgacctgc accggcacca gcggacagcc gcggcgcagg gcgagccacg cggggccgat
    12301 cgtggtcggc gcgaggccac cgaaaaacgg tactaattcc cctgaatccg tgcgctggtc
    12361 gggcagcatg cccacggatc ggccggcctc gaggtcgctc aggagccggc gcatgcagtt
    12421 ggccttgccg aggaaacggc agccgagcgg cgcgcgccgc gcctggatca tcgcgtcgac
    12481 gtacgggttg ccctgcggcg cgtaaacggc gctcagcgcg atccccagcc gaccgatcgc
    12541 cgcggtcgcg agctcccagt tcgcgaggtg cgcggcgacg tagatggcgg gctcgcggcg
    12601 agagacgatg tcgcgcgtct cgggggcgat cgccacgcgc aggaagggcg agggatccgg
    12661 cgcgcaaaat cgcggcagat gcgggtattc cgcgaggatc gcgccgaaat tcgcccaggc
    12721 ttcgcgcgcc agggcctcga tgcgcgcggg cccggcggca gggaagagcc gtgccaggtt
    12781 cgacctgacg aaggcctgct tgcgcaggcg cggaccgagc caggcgaaca ggcgcgcgcc
    12841 ggtgcgcgcg gcacgccgcg gcggcaatgc gcgcgcgccc agccagaaca gtccgagcgc
    12901 ggccgcctcg atgcggcgca cgggctcgcg ccaacgtgaa tcgcggattc tcgcctcgct
    12961 cgaaaacatc cttcgtaccc ctgccaatgg cttgcgatgg aggcaaggta gcaactcggc
    13021 cgcgcgccga cagtcgaccc ggggggttga tttccggggc gcgagaccgt gactaagctc
    13081 gatccgccat caccaccgga cgagacgcac gatgttcgat ctgcatgccg gcaccacgca
    13141 ccgcggcaac caccacccac gcgtgtggct cctcaacggc ccccggcccg gcgacaacaa
    13201 ccaggtgctc gggctcggcg aaacgctggg ctggcccttc gaactgaagt ccgtcggctt
    13261 ccactcctcg ccgccggtgc cgaagatgat ctcgagccaa ctccgcagcc tggcgctcgc
    13321 cggtgtcgac cggcgccgca gcagcgcgct gcaggcgccc tggccagacc tcgtgatcag
    13381 cgcgggccgc gaactggagc cggtggcgcg gtggatcaag aaacgatcgg gcaaccgcgc
    13441 gcggctggtg cacatggggc gcccgtggtc gcgactggat gccttcgatc tcatcatcag
    13501 cacgccgcag tacttcctgc ccgagcgcga gaacatcctg ctgaacgagt tgccgctgca
    13561 ccgcgtcacc gagcggcggc tcgaggaggc gcgcgcgctg tgggcgccgc gcttcgcgca
    13621 tctgccgcgc ccctatacgg tggtgctgct cgggggcaac agcggctcct tcgtgctctc
    13681 gtgcgcgaag gcgcgccggc tcggcaagct ggtgaacgcg ctgacgcgcg gcagcggcgg
    13741 ttcgctgctg gtcaccagca gcgcgcgcac gcccgcggac gcgtaccacg acttcctcga
    13801 cggtatcggc ggcccgcggc acgaacatca ctggggcgcg caggaaaacc cctacttcgg
    13861 ctacctcgcg ctcgccgacc gcatcgtgat caccggcgag agcgtatcga tgctcgcgga
    13921 ggccaacgtc accggcaagc cgctgctgat ctacgacatc gccgagcgcc ccgcgcccga
    13981 catgccgccg tggcagcggc tcgcgatccg gctgagctac cgttcgctgc gcgacctgtt
    14041 cgcgcgcacc atcggcgcgc cccacatgca gcgcgatatc ggcgcgatcc agcgtcacct
    14101 ggtcagcatc gggcgcgcgg cgtggctcga cgaggccagc ttcgggagcg cggcgtcgca
    14161 gcagccctgg cacgcgccgc gcctcgatgc gcagcgcgcc gcctcgcgcg tgcagaacct
    14221 gctcgcaggc gtgctgccat gttgacggcg cgggcgctga tgacgctgct gagggccatg
    14281 atgggggtgg ccgcgaacct gctcctcgcc gtgctgctgc actggcggga gaccgagagc
    14341 ctgtaccagg agaacaacct gctcgagaac ctgcagctcg cggcgctcgc cggctgcgtg
    14401 ctggtctacg ggttgcgcgc gcgccggggc gtcgacggct atgcccggct gcagctcggc
    14461 gccctcgcgt tgctcagcgc ctgcatgttc taccgcgaga gcgatttccg ctggctgctg
    14521 ccggaaggca cgctcgtcta tgccatcgcg tacgtggtcc atcgcggcgc cttcgtgttg
    14581 gcgctgctcg gcgtggcggg ggccctggcg cgttgctggc tgctcgaccg gcgtcgcttc
    14641 ctggatttcc tcgactccgg ccccggcatg ctgctggtgc tggccacggt gctgctggcg
    14701 ggcggcgtgc tgctcgacaa gagcgtgatc agcatcgccg cgcccaatcg cctgttcgag
    14761 gaactcttcg aactgaacgc ctacgggctg atgctcatcg ccggcggcag ccgcgcgctc
    14821 ggacggccgc ccgcgcggca cgcgcccgga tcctccggcg tgcccgctgc cgggcccgcc
    14881 cgcaccgtgc cgctcgaccg atgaatgtgc cgccctgcgc ctgcgcactg ccatcggaaa
    14941 gctgcccgcg cttctcgccc gcgacgcgcg ccggcgcact ggcgcggatc gggctcatca
    15001 gcaacgccaa cagcggctgc aaccggctgc acctcgcggg tgtgcaccgc atcctctcgc
    15061 gccacgccgg cctgcgccac gtcgtgaccg gcagcgccga cgaggtgcga cacgccgtcg
    15121 ccgaactggc gcgcgagcac atcgacgtac tggcgatcaa cggcggtgac ggcaccgccg
    15181 cgagcgtgct cggtcatgtg ctcgcccagg ctcccttcgc gacgcacccg cgcgtcgtgc
    15241 tgcttcccgg gggcaccgtg aacatggcgg cgacggcgat cggcacgggc ggcgacctgt
    15301 tgcgcgcggc gacgcggctc agcgggtgga tctccgaacg cccgcgtccc tgtcgcgagg
    15361 tgcgccagtc gatcctgcgc gtgcagctgg gcgaccacgg cgcggcgcac cacggcatgt
    15421 tcctgggcgc gggcgccgtg atcaagggaa ccgaatacgc gcaccgcagg gtgcacgcga
    15481 tggggctgcg cggcagcgaa agcctcggcc tcgcgctgac gcgcacgctg tgggggctgc
    15541 agcgccgcga tccgcgtttc gcgcatgccg tgccggtgac gctgacgctg gacgacgcgc
    15601 cgccgctgcc gcagcaggac gccctgatcc tgctggtcag cagcctgcag cgcctgtcgc
    15661 tcggcatgtg tcccttctgg ggcagcgaga acggtgcggt gcgcctgacg ctgatcc
//
LOCUS       DAORUQ010000063        15658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3525_length_15658_cov_5.516183, whole genome shotgun sequence.
ACCESSION   DAORUQ010000063 DAORUQ010000000
VERSION     DAORUQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_15658_cov_5.516183"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLW13_07450"
     CDS             complement(<1..590)
                     /locus_tag="PLW13_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQY70245.1"
                     /translation="MTRQIRFGGWCFDPDTHLLTMGEERRMLEPRVSRLLEFLLAHPG
                     ETLSHDQLVDAVWDGRVVSDEAVRRAVSTLRHALAPDGSDHCIRTVHKKGYVALFDAS
                     QAQAATPRAQRGASSPQRYVPPLWQRIAWALSLVFLASALTLTLLAYRRTSEPPPQSQ
                     AAAVAPTLAVLPFINLSAEADTDFLADGVSEELRDTL"
     gene            805..1371
                     /locus_tag="PLW13_07455"
     CDS             805..1371
                     /locus_tag="PLW13_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HQY70246.1"
                     /translation="MPLSGIRGPIVLQCAGGLEAARQAQVMAPAIARGRPGKIRRGVM
                     KGQMRHWLFAAGALALGAALGIPAQAARNPENMTVSGAAKRPMEQVQTARRQTVTRSI
                     VVAYGDLDLSAQSGTQKLYVRLRGAARTVCSPRETRITRLRREWAECVDIALDNAVAA
                     TGIERVVAMHRDATGRDVSVTTQLAGSP"
     gene            complement(1418..>2623)
                     /locus_tag="PLW13_07460"
     CDS             complement(1418..>2623)
                     /locus_tag="PLW13_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HQY70247.1"
                     /translation="YYGNFLSELGSGGRDYLQTQALQAAVSGGLNAYTTIRGGVADEQ
                     QTRGVLVISSKALGRNADSMFALVRETLDSARFDELQRLRELLSQMRARRDQSITGSG
                     HSLAMAAASSRMSPSALLGHTLSGLEGIRRTRALEKSLQSDAELAAFAARLAELHALL
                     RAAPRQLLLVTDADHTTSTVGALQACWHDTAAATTTAPFALAPVRAGVREIWQTSTQV
                     NFCAKAYPTVPMGHPDAAALTVLGGFLRNGFLHRAIREQGGAYGGGASHDAGIAAFRF
                     FSYRDPRHGATLDDFDASLAWLQDTKHEWQQVEEAILGVISSLDKPASPAGEAKKHFH
                     ETLFGRTIAARREFRAQVLAVTLDDLLRVGATYLDPAKASIAVVTNAPGAEELRRHAA
                     LVDAELKTL"
     assembly_gap    2624..2723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2724..4186)
                     /locus_tag="PLW13_07465"
     CDS             complement(<2724..4186)
                     /locus_tag="PLW13_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQY70248.1"
                     /translation="MNSIQMPATRTAPATVHPAFEWVRSEPIVALGAVVEEYRHRVTG
                     AQHFHIATDNPENVFLVALRTVPGDSTGVAHILEHTALCGSEKYPVRDPFFMMTRRSL
                     NTFMNAFTSSDWTAYPFASQNRKDFFNLLDVYLDAVFFARLDPYDFAQEGHRLEFATP
                     TDPSSPLQFKGVVYNEMKGAMSSISSTLWQTLSKYLFPYTTYHYNSGGDPEHIPDLTY
                     EGLCEFYRTHYHPSNAVFMSYGDLAVPELHERFESRALARFEALPVHIAVPDEKRYAA
                     PVAVEEYYAWEEEGAPEEKSHIVLGWLLGKSTSLEDLLKAHLLNGILLDNSASPLQQA
                     LETTELGSAPSPLCGLEDSNREIVFSCGLEGSDGSWAQEVEALVLDTLRDVAENGVPI
                     EQVRAVLHQLELHQREIGGDGYPFGLQLLLMAMSGAVHRGDPVAVLNLEPVLEKLHRD
                     IEDPDYVKRLVRELLLDNPHRVRLVMNPDTQLAARRRA"
     gene            4383..5360
                     /locus_tag="PLW13_07470"
     CDS             4383..5360
                     /locus_tag="PLW13_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HQY70249.1"
                     /translation="MTTRSRKALAFVIPALLCSELALAQAVVLKESEQKTERKAGKEA
                     GKEPSPFSLEISAGLQADSNVSVNELDANTGAEDQAAVFDADLDYELALGESTELQLG
                     YGLSQTSYDDFSEFDLQNHLLTGEVARDFGDFDAGVAYRYIHTRLGGHDYMKTQQASP
                     SVSRFFGRRLFARAEYTFSDKDFDEINERDATTHAGGADLYWFLNGVRTYVVLGYRYR
                     DENAEDAQFDFGSNNLKLRLSQRFRFLERDATLKLGWLYEQRDYSSVTPAIGEHRDDD
                     RNRFQAELELPLGERFFTVLDYEYGGFSSNLDSADYNQHVASIRVGLKI"
     gene            complement(5338..7374)
                     /locus_tag="PLW13_07475"
     CDS             complement(5338..7374)
                     /locus_tag="PLW13_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY70250.1"
                     /translation="MRMHRIIPTALLGLILVVGLPGLPLPGGAHARAWAGDDGIEDAI
                     DAQTEDEISEQVEDAVEEDIEDQVSDRVEEEIDAEVEDRVEESIQSDVEDSVENDVED
                     SIEAEVEDRVEADVEDSVEADVEDRVEDAVEDSVEADVEDAVESDVEADVEEDVEQDV
                     EEDVEDEIESDVEDDLEAEVEQDVEDDMEDDLESDIEENVEDDLEGDVEDAMENELES
                     EIEDDMELEFDDEPELEPGDDLESDVDDAMEDAAPEDAGRDAVVDSRQPALDEPGGKR
                     SPARGAAATESAPRPAAVAGTVNSVQPGQASQTARAAAEHFAGLDADDEGNEISLGEW
                     LVIASRDDIAALEALGYVTRNVEPLEGLDRVLATMEAPESFDIAAAQAAIGRAAPQAE
                     VDYNHIYRPEARQQPPASGGGSPGELFPAAAGVRTPSAPIGLIDTAIDAGHPALRAAN
                     VQYRDFTPPGQPRPSAHGTSVLSILVGEAPGYRGLVPGAAVLAGAVFFNGPDGREIAT
                     TSSLVRALDWMTVNRVPVVNMSLAGPRNAILATAIDRSFDTGTVVVAAVGNRGPAARP
                     LYPAAYERVIAVTAVNGSRVYRLANRGAHVDFAAPGVDVLHADREAGYRSSSGTSLAA
                     PFVTAVIATSCADVRPIDACLTALQRSAEDIGEAGFDPVFGHGLIVPLRSSAPP"
     gene            7589..8116
                     /locus_tag="PLW13_07480"
     CDS             7589..8116
                     /locus_tag="PLW13_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY70251.1"
                     /translation="MNADALRRELLGLLPRLRRYAYALTGARHDADDLLQATLERLLV
                     KGVPEAVEVDKWAFRVCRNLWIDEIRARRVRSAVPLEESGAMEQTFDGENALMSKLAL
                     AEISDAMDELPEEQRAALSLVVLEGLSYAQAAEVLETPIGTIMSRIARARAALSKALR
                     AESPPIGAGLATGAG"
     gene            8116..8892
                     /locus_tag="PLW13_07485"
     CDS             8116..8892
                     /locus_tag="PLW13_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQY70252.1"
                     /translation="MITNDMLSAYIDGELAPEEMERVARAIAADELLAARLEQFRRVD
                     RLLTEFSSAIDAQPLPPAVLALLEPPCADDDAAARAAPRAVVLPLTRRPLHRRAAALA
                     LAASLVMAVAVVLQFDARDGAPTGFDELVRAGAVVPSSPLQHVLEEVPSDEAYAVAGG
                     TGVKITPVLSFVSRGQEYCREFRVDAGARAARGVACRRDERWETLKVVAAEARGSDAH
                     YATATAETDEDFDAFVDGLVADEPLGADAEARALHGGWTR"
     gene            complement(8889..9728)
                     /locus_tag="PLW13_07490"
     CDS             complement(8889..9728)
                     /locus_tag="PLW13_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HQY70253.1"
                     /translation="MAALRRCRRRLAAVLVLAGLAPVACGEADTRTDPLAAIPRLPFT
                     VEQRLPHDHTLFTQGIAFAGELLVESGGGYRESRLVVRRLDEPRPLHSVDLPRRWFAE
                     GIAPHQGQLFLLTWREGVAQSFSLPQLHPGATFRYAGEGWGLTSDGSQLIQSDGSNVL
                     TWRDPRDFSVTRRLAVHAGSTPLAQLNELEWINGWIVANVWRSDYVVGIDPASGCVRW
                     KLSLENLLDERERRSSDVLNGVAWEAGAQRLWVTGKRWPWLFGLRVELPALGAAATDD
                     CPG"
     gene            complement(9728..11785)
                     /gene="mnmC"
                     /locus_tag="PLW13_07495"
     CDS             complement(9728..11785)
                     /gene="mnmC"
                     /locus_tag="PLW13_07495"
                     /inference="COORDINATES: protein motif:HMM:NF002481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HQY70254.1"
                     /translation="MRRVIIRPMSSDLPPPIATPRASEPEWLADGTPFSREFNDVYYS
                     RDDGSAEARYVFLEHNRLAARWAALPDAPGTLFVIHETGFGTGLNFLLAWQLWQRVAP
                     ARARLVFRSLEAFPLEAGSLARALQRWPELDPFTRQLLGAWPPLLPGLHTLEFDAGRV
                     SLQLYIGDVREALEQYGADTHPELAVKADAWFLDGFAPARNPAMWDDAVMRRVAALSK
                     PGTTLATFTVAAAVRAALAMHGVTLARVPGFGRKRDMLTGVFGAKSAPEPQPPTQTPW
                     HLAAAASQGDRRAIVIGAGIAGATCARALARRGWRVTVLEAHAAPAAGASGNPQGMLF
                     TQLPAADTPHGEFTLHSFLHALRYYAELFAGDSEGFSPCGMLQLHGPQDAAALARLAE
                     RFAAYPRLVRLVDAAQASALAGVPLAHGGLYLPASGWIVPPAACARALDHPLIETRPG
                     WRASALSAEESGWRVQLDAGATLQASAVIVATAAAANELLGDDALPLSPLRGQITLLP
                     RAALTPAPRCVVCAEGYVAPPYGERICCGASFVRGAGDFVPTAAEHCGNLQRLQEILP
                     YFDAASIDVATLAGRVGYRASTPDRLPVAGPVPDIAAFREAYAGLQRNARLPIDRIGP
                     YRRGLYVSAGHGSRGLTSAPLCAEAIAAAIAGEPSPLSRRLQRTLSPARFLIRRIIKG
                     QLP"
     gene            11846..13456
                     /locus_tag="PLW13_07500"
     CDS             11846..13456
                     /locus_tag="PLW13_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY70255.1"
                     /translation="MRLRPLILLLACLAGGTSYAAPGEAVDAASGTVRLLLSAEPPSL
                     DSVRATDQVSFFVLSHLMDGLLQYDADNRLAPALAERWELRPDGATFWLRRGATWEDG
                     APVTAHDFVHAWREVLRPANAAPYASLLYPVRNARAIASGELAPESLGATAPEDHRLE
                     VVFGQPCAWFPALTAFMTLLPLRADFHAAQGGRHAADAGRLLANGPFRLTRWVHGAQL
                     VLEKNPRYWNAGAIRLQRIEVPYITTDAQAELNLFLDRRIAMANIAPDSVRQVLLERQ
                     RLRRFADGYVHFLTFNFRDGRPTQNLRLRRAIQAALDPGEIVNRVLRMPGLRPAESLF
                     PSVLRGRERAFVDEYPLAAPARGDAIAQREFAAALAELGVTELPALHLLTGEAPTALR
                     IGEYAQARLAETLGVRLRLDRQITKQRLQQMARGDFDIALQNWGPDFDDPVTFAELLA
                     SGNPLNRGQYRSAGYDRWLGVALGSTEQAQRLAAFDALQRLVRDEAPLIPVYENARLY
                     VQHPALQGVVRAPFFGDPNLRHAWIGTR"
     gene            13525..14976
                     /gene="cls"
                     /locus_tag="PLW13_07505"
     CDS             13525..14976
                     /gene="cls"
                     /locus_tag="PLW13_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HQY70256.1"
                     /translation="MSFPLPDPELLRIVALACGMSVTLLGAGHALLTKRDPRSAAAWV
                     SLCVFFPVVGVLAYLALGNNRIRTRARRLHALPPHEQPAPPPAREHVAAEHLIAPQYR
                     NLAYLGLAASGNELLTGNSVEALYCGENAYPAMLLAIREARRHVYLATYIFDSRGIGA
                     EFVEALGAAQARGVQVRVLLDGFGEFYSLPRVGWRLRAAGVTTARFLQPRLWPPNFTL
                     NLRNHRKILVVDGELAFTGGMNIRNKHIAVRGVRPADVIDVHFRLRGPIVTQIEEVFA
                     ADWLFSTRQALPPPPSVAPVTMLPEEAECRVIVDGPNEDLDKLLWVLVGAISAARSSI
                     RIMTPYFLPPPELAVALQTAALRGVEVTIVLPAHNNFPFMTWATTHMLGELLKTGVRV
                     HFYTGPFLHTKLLLVDDYYAQIGSSNLDPRSLRLNFEIAVEIYDHVFGRGVAVHFAEM
                     LATSRELSFSTWQERSLPRRLRDAVFWVFSPYL"
     gene            15044..15526
                     /locus_tag="PLW13_07510"
     CDS             15044..15526
                     /locus_tag="PLW13_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70257.1"
                     /translation="MSQSQSVSMESQRFFTGALNVLHAGFIRASRAQAKRHYARVHGG
                     GVLELAKLKLGDGSDLLFRVALDQSEFRGKLDFATFRKALVQLLAKLQDRIRFKGELN
                     IYRNDETGAMLLNLPSILTEDGRTNVMMLGVDPPEPGVVTLRLQFLDPEQFRQPAATA
                     "
BASE COUNT         2306 a         5311 c         5470 g         2471 t
ORIGIN      
        1 agcgtgtcac gcagttcctc ggacacgccg tccgcgagga agtcggtgtc ggcttccgcg
       61 ctgaggttga tgaacggcag caccgcgagc gtgggggcaa cagccgccgc ctgggactgg
      121 ggcggcggtt cactggtgcg acggtaggcc agcagcgtga gggtgagtgc gctggcgaga
      181 aagaccaggc tgagcgccca cgcgatgcgt tgccacagcg gaggcacgta gcgttgcggg
      241 gatgatgcgc cgcgctgtgc ccggggcgtc gcggcctgcg cttgcgacgc atcgaacagc
      301 gccacgtagc ccttcttgtg cacggtgcgt atgcagtggt ccgagccgtc cggcgccagc
      361 gcgtggcgca gcgtgctcac ggcgcggcgc accgcctcgt cggacaccac ccgtccatcc
      421 cacacggcgt cgaccagctg gtcgtggctg agagtttcgc ctggatgcgc gagcaggaac
      481 tcgagcaggc gtgacacgcg gggttcgagc atgcgccttt cctcgcccat cgtcaggaga
      541 tgggtatcgg gatcgaaaca ccagccgccg aagcggatct gccgtgtcat tgcgatccgt
      601 ccctgtgcgc gccaggacaa cgaaggggag aacgggagtg agctcgggtt caaggcgcgg
      661 cgagccaacg gagcaaatat gcgccggatt gttctttgat tccactgcaa atgtgatcgc
      721 atgaactcac aatcagaagg gcccggtcgc gcgcgcgtcg caatcgattg attttcaaga
      781 aatcagaatc atcacgaaaa aatcatgccg ctctccggca tccgcggtcc gattgtgctc
      841 cagtgcgccg gcggactgga agctgcgcgt caggcgcaag tgatggcgcc agcgatagcc
      901 aggggcagac ctgggaagat aaggagagga gtcatgaaag gacaaatgcg gcattggttg
      961 tttgcggccg gcgcgctggc gctgggcgcg gcgctcggca ttccggcaca ggccgccagg
     1021 aacccggaaa acatgaccgt gagcggcgct gcgaagcggc ccatggaaca ggtgcagacg
     1081 gcgcgccgcc agacggtcac acgctcgatc gtggtggcgt acggtgatct ggacctgtcg
     1141 gcacaatccg ggacgcagaa gctgtatgtg cgcctgcgcg gcgcggcgcg aacggtgtgt
     1201 tcaccacgcg aaacacgtat cacgcgcttg cgtcgcgagt gggcagagtg cgtggacatc
     1261 gcgctcgaca acgcagtggc tgcgacgggc atcgaacggg tcgttgcgat gcaccgtgac
     1321 gcgacggggc gcgacgtgtc ggtcaccacg cagctggcgg gctcaccctg aaattgcggg
     1381 gacagggcgc tttgccccgc ccccggttgt tcagggttca gagtgtcttc agctcggcat
     1441 cgacgagcgc cgcgtggcgg cgcagttcct cggcgccagg ggcgttggtc accaccgcga
     1501 tgctggcctt cgccgggtcg aggtaggtgg cgcccacgcg cagcaggtcg tccagcgtga
     1561 ccgcgagcac ttgcgcgcgg aactcgcgcc gcgcggcgat cgtgcgtccg aacagcgttt
     1621 cgtggaaatg cttcttggcc tcgccggccg gtgaggccgg cttgtcgagg ctgctgatca
     1681 cgccgaggat cgcttcctcg acctgctgcc actcgtgctt cgtgtcctgc agccaggcca
     1741 gcgaggcgtc gaagtcgtcc agggtcgcgc catggcgcgg gtcgcggtac gagaagaagc
     1801 ggaacgccgc gatgccggcg tcgtgcgagg cgccgccgcc gtaggcaccg ccctgctcgc
     1861 ggatcgcgcg gtgcaggaag ccgttgcgca ggaagccgcc cagtaccgtc agcgcggcgg
     1921 catcgggatg gcccatcggc acggtggggt aggccttggc gcagaagttc acctgggtgc
     1981 tggtctgcca gatctcgcgc accccggcgc gcacgggcgc cagcgcgaac ggcgcggtag
     2041 tggtggccgc ggctgtgtcg tgccagcagg cctggagcgc gccgaccgtg ctggtcgtgt
     2101 gatcggcatc ggtgaccagc agcagctggc gcggtgcggc gcgcagcaag gcgtgcagct
     2161 cggccaggcg cgccgcgaag gcggcgagct cggcgtcgga ctgcagggac ttctccagcg
     2221 cgcgggtgcg gcggataccc tccagccccg acagcgtgtg ccccagcaag gccgaggggc
     2281 tcatgcggct cgaggccgcc gccatcgcca ggctgtggcc gctgccggtg atcgactggt
     2341 cgcggcgcgc gcgcatctgg ctcagcagct cgcgcagacg ctgcagttcg tcgaagcgcg
     2401 cgctgtcgag cgtctcgcgc accagggcga acatgctgtc ggcgttgcgt cccagcgcct
     2461 tcgaggagat caccagcaca cccctggtct gctgctcgtc ggccacgccg ccgcggatgg
     2521 tggtgtaggc gttcaggccc ccgctcaccg cggcctgcag agcctgcgtc tgcaggtagt
     2581 cgcgcccgcc gctgccgagc tcggagagga agttgccgta gtannnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnngcgcgcc ggcgcgcggc cagttgcgtg tcggggttca
     2761 tcaccaggcg cacgcggtgc gggttgtcca gcagcaactc gcgcaccagg cgcttgacgt
     2821 agtcgggatc ctcgatgtcg cggtgcagct tttccagcac cggctcgagg ttcagcaccg
     2881 ccaccgggtc gccgcggtgc acggcgcccg acatcgccat cagcagcaac tgcaggccga
     2941 agggataacc gtcgccgccg atctcgcgct ggtgcagttc cagctggtgc agcaccgcgc
     3001 gcacctgctc gatcggcacg ccgttctcgg ccacgtcgcg cagcgtatcc agcaccagcg
     3061 cctcgacctc ctgcgcccag ctgccatcgc tgccctcgag gccgcagctg aacacgatct
     3121 cgcgattgga gtcctccagg ccgcacagcg gcgagggcgc gctgccgagc tcggtggtct
     3181 ccagtgcctg ctgcagcggc gaggcgctgt tgtcgagcag gatgccgttc agcaggtgcg
     3241 ccttcagcag gtcctcgagg ctggtcgact tgccgagcag ccagccgagc acgatatggc
     3301 tcttctcctc gggcgcgcct tcctcctccc aggcgtagta ttcctcgacc gccacgggcg
     3361 cggcgtagcg cttctcgtcg ggcaccgcga tgtgcaccgg caaggcctcg aagcgtgcca
     3421 gcgcgcgcga ctcgaaacgc tcgtgcagct cgggcaccgc gaggtcgccg tagctcatga
     3481 acaccgcgtt gctcgggtga tagtgcgtgc ggtagaactc gcacagccct tcgtatgtca
     3541 ggtccgggat gtgctccgga tccccgcccg agttgtagtg gtaggtggtg tacgggaaca
     3601 ggtacttcga cagcgtctgc cacagcgtgc tcgagatcga gctcatcgcg cccttcatct
     3661 cgttgtacac cacgcccttg aactgcagcg gcgaggacgg gtccgtcggt gtcgcgaact
     3721 cgaggcggtg gccctcctgc gcgaagtcgt agggatcgag gcgcgcgaag aacaccgcgt
     3781 cgaggtagac gtcgagcagg ttgaagaagt ccttgcggtt ctggctcgcg aaggggtagg
     3841 cggtccagtc gctgctggtg aacgcgttca tgaaagtatt cagcgagcgg cgcgtcatca
     3901 tgaagaaggg gtcgcgcacg gggtacttct cgctgccgca cagcgcggtg tgctccagga
     3961 tgtgcgccac cccggtggag tccccgggca cggtgcgcag cgccaccagg aacacgttct
     4021 ccgggttgtc ggtggcgata tggaaatgct gcgcgccggt gacgcggtgg cggtattcct
     4081 cgaccacggc gcccagcgcc acgatcggct cgctgcgcac ccactcgaag gccgggtgga
     4141 cggtcgcggg ggcggtgcgg gtggcgggca tctggatact gttcatgctg ggtggagtac
     4201 ttccggtaaa ttggttcgga cttcggcagg cgcggcagcg gggccggcag tgcggacggg
     4261 gccggcgctc tggcgctccc gctcccgtcg aggcgaaggt taccagagcc gggaattcgg
     4321 ggacagttta ctcagctgca attggctaaa ctgcacctga aatccgagac aggagagcgc
     4381 acgtgacgac ccgttcaagg aaggcgcttg ccttcgttat ccccgccttg ctgtgcagcg
     4441 agctcgctct ggcgcaggcc gtggtgctga aggagtccga gcagaagacc gagcgcaagg
     4501 ccgggaagga ggcgggcaag gagccatccc cgttcagcct ggagatcagc gccggcctcc
     4561 aggcggacag caacgtgtcc gtgaacgagc tcgatgccaa caccggtgcc gaggaccagg
     4621 ccgcggtgtt cgacgccgac ctcgactacg aactcgcgct cggcgagagc acggagctgc
     4681 agctcggcta cggactgtcg cagacctcct acgacgattt ttcggagttc gacctgcaga
     4741 accatctgct gacgggcgag gtcgcacggg atttcgggga tttcgatgcc ggcgtggcct
     4801 accgctacat ccacacccgg ctgggtggcc acgactacat gaagacgcag caggcctcgc
     4861 cgagcgtctc gcgcttcttc ggcaggcggc tcttcgcgcg cgccgagtac accttcagcg
     4921 acaaggactt cgacgagatc aacgagcgcg acgccaccac gcacgcgggc ggtgcggacc
     4981 tctactggtt cctgaacggc gtgcgcacat acgtcgttct cggctaccgc taccgtgacg
     5041 agaatgccga ggatgcgcag ttcgatttcg ggtcgaacaa cctcaagctg aggttgtcac
     5101 agcgtttccg cttcctggaa agggatgcga cgctcaagct cggctggctc tacgagcaac
     5161 gcgactattc ctcggtcacg ccggccatcg gcgagcatcg cgacgacgac cgcaaccgct
     5221 tccaggccga gctcgagctg ccgctgggcg agcgcttctt taccgtgctc gattacgaat
     5281 acggcggatt ctcgtcgaac ctcgattcgg ccgactacaa ccagcacgtc gccagcatca
     5341 gggtggggct gaagatctga gcggcacgat gaggccgtgt ccgaacaccg ggtcgaagcc
     5401 ggcctccccg atgtcctcgg cgctgcgctg cagcgccgtc aggcacgcat cgatcggccg
     5461 cacgtcggcg caggacgtcg cgatcaccgc ggtcacgaag ggcgctgcca gcgaggtgcc
     5521 cgaagagctg cggtagcccg cctcgcggtc ggcgtgcaaa acgtccacgc ccggtgccgc
     5581 aaaatccacg tgtgcgccgc gattggcgag ccggtagacc ctcgaaccgt tgacggccgt
     5641 caccgcgatc acgcgttcgt aggcggccgg gtagagcggc cgcgcggccg ggccgcggtt
     5701 gccgacggcc gcgaccacca cggtgccggt gtcgaatgag cgatcgatgg ccgtcgcgag
     5761 gatggcgttg cgcggccccg ccaggctcat gttcacgacg ggtacgcggt tgacggtcat
     5821 ccagtcgagc gcgcgcacca ggctcgaggt ggtggcgatt tcccggccgt cggggccgtt
     5881 gaagaagacc gcgcccgcaa ggaccgccgc gcccggcacc aggccgcgat agccgggcgc
     5941 ctcgccgacc aggatcgaca gcaccgaggt gccgtgggcc gagggccgcg gttgcccggg
     6001 aggcgtgaaa tcccggtact ggacgttcgc cgcgcgcagg gcggggtggc ccgcgtcgat
     6061 ggcggtgtcg atgaggccga tcggggccga cggcgtccgc acgcctgctg ccgcagggaa
     6121 cagctcgccc ggtgagccgc cgccactcgc aggcggctgc tggcgggctt cgggccggta
     6181 gatgtggttg tagtccactt ccgcctgagg cgcggcgcga ccgatcgcgg cctgcgccgc
     6241 ggcgatatcg aagctctcgg gcgcctccat ggtggcaagg acccggtcga gtccctccag
     6301 cggctccacg tttcgcgtga cgtatccgag tgcctccagg gccgcgatat cgtcgcgcga
     6361 ggcgatgacc aaccactcgc cgaggctgat ctcgttgccc tcgtcgtctg cgtcgaggcc
     6421 ggcaaagtgc tccgcggcgg ccctggcagt ctgcgacgcc tgtcccggtt gtacgctgtt
     6481 gaccgtgccg gcgacagcgg caggccgcgg cgcggattcc gtcgcggcgg cgccacgcgc
     6541 cggcgagcgc ttgccgccgg gttcgtccag cgcgggttgg cggctgtcga cgacggcatc
     6601 gcggcccgca tcttccggcg ccgcgtcttc catcgcgtca tcgacatcgc tttccaggtc
     6661 gtcaccaggc tcgagttcgg gctcgtcatc gaactcgagc tccatgtcgt cctcgatctc
     6721 gctttcgagc tcgttctcca tggcgtcttc gacgtcgccc tcgagatcgt cctccacgtt
     6781 ctcctcgata tcggactcga gatcgtcctc catgtcgtcc tcgacatcct gctccacctc
     6841 tgcctcgaga tcgtcctcga cgtcgctctc gatctcgtcc tctacgtcct cctccacatc
     6901 ctgctcgacg tcttcctcga catccgcttc cacatccgac tcgacggcgt cttcgacgtc
     6961 tgcctccacg ctgtcctcga cagcgtcctc gacgcggtct tccacgtccg cctcgacgct
     7021 gtcctcgaca tcggcctcga cgcggtcttc tacctctgcc tcgatgctgt cctcgacatc
     7081 gttctcgacg ctgtcttcca catccgactg gatgctctcc tccacgcgat cctcgacctc
     7141 ggcatcgatt tcctcttcga cccggtcgct gacctggtcc tcgatgtctt cctcgacggc
     7201 gtcttccacc tgctcgctta tctcgtcctc ggtctgcgcg tctatcgcat cctcgatgcc
     7261 gtcgtcaccg gcccaggcgc gtgcgtgagc gccgcccggc aggggcagtc cgggcagtcc
     7321 aaccacgagg atcaggccga gcagggcggt ggggatgatt ctgtgcattc gcatcgacag
     7381 cggttcgttg ggcggctccg gatggtgcag gcgcgtgtat cgggggatcc gaccaggcgg
     7441 caaggctgcg ccatgttacc ttagtaatga acgtgtggag ccggcacaaa attccccgcg
     7501 ctgcgtggaa taaacgccgg acctgattcg tcatcgagtt aaacgggacc ggcatggcac
     7561 catcgcgaat gcccacggac accagggaat gaacgccgac gcgctgcgcc gggagctgct
     7621 cggcttgctg ccgcggctac gccgctatgc gtatgccctg accggcgctc gccacgacgc
     7681 cgacgacctg ttgcaggcga cgctcgagcg cctgctcgtg aagggcgtgc cggaggcggt
     7741 ggaagtcgac aagtgggcct ttcgcgtctg ccggaacctg tggatcgacg agatccgcgc
     7801 gcgccgggtg cgctcggcgg tgccgctgga ggagtcgggt gcgatggagc aaaccttcga
     7861 tggggaaaac gccctgatga gcaagctggc cctggcggag atcagtgatg cgatggacga
     7921 actgcccgag gagcagcgcg cggcgctgtc gctggtcgtg ctggaggggt tgtcctacgc
     7981 acaggcggcc gaggtgctcg agacgccgat cggcaccatc atgagccgca tcgcacgggc
     8041 ccgggcggcc ctgtcgaaag ccttgcgcgc ggaatcgccg ccgatcggcg caggcctggc
     8101 aacgggagca ggatgatgat cacgaacgac atgctgtctg cgtatatcga cggcgagctc
     8161 gcgccggagg agatggagcg ggtcgcccgg gcgattgccg ccgacgagct gctagccgcg
     8221 aggctggagc agttccgccg cgtcgatcgg ctgctcacgg agttcagcag tgccatcgac
     8281 gcccaaccgc tgcctcccgc ggtgctcgcg ctgctggagc cgccgtgcgc cgatgacgat
     8341 gctgccgcac gcgccgcgcc cagggcggtg gtgttgccct tgacgcgccg gccgctgcac
     8401 cggcgagcgg ctgcgctggc gctggccgcg agcctggtga tggcggtggc ggtcgtgctg
     8461 cagttcgacg cgcgcgacgg tgcgccaacc ggcttcgacg agcttgtccg cgccggagcg
     8521 gtcgtgccgt ccagccccct gcagcatgtg ctggaggaag tgcccagcga cgaagcctac
     8581 gcggtggctg gtggcaccgg cgtcaagatc acccccgtgc tgagcttcgt gtcgaggggg
     8641 caggagtact gccgcgagtt tcgcgtggat gccggggcgc gcgccgcgcg cggcgtggcc
     8701 tgccgtcgcg acgagcgctg ggagaccctg aaagtcgtcg ccgccgaggc gcgtggcagc
     8761 gacgcgcact acgcgaccgc gacggcggag acggacgagg atttcgatgc cttcgtcgat
     8821 ggcctggtgg cggatgagcc gttgggcgcg gatgccgagg cgcgcgcgct gcacggcggt
     8881 tggactcgct aacccggaca gtcgtccgtg gccgccgcgc cgagggcggg caactcgacc
     8941 cgcaagccga acagccaggg ccatcgcttg cccgtcaccc agagccgttg cgcgcccgct
     9001 tcccacgcaa caccgttcag cacgtcgctg ctcctgcgct cgcgttcgtc cagcaggttt
     9061 tccagcgaca gtttccaccg cacgcagccg ctggccggat cgatgccgac cacgtaatcg
     9121 ctgcgccaca cgttggcgac gatccagccg ttgatccact cgagctcgtt cagctgcgcc
     9181 agcggggtgc ttccggcatg cacggcgagg cgtcgtgtca cggagaaatc ccgcggatcg
     9241 cgccaggtga gcacgttgct gccgtcgctc tggatcagtt gcgagccgtc gctggtcagt
     9301 ccccagcctt cgcccgcgta cctgaaggta gccccgggat gcagttgcgg caggctgaag
     9361 gattgcgcca cgccctcgcg ccaggtgagc aggaacagct gtccctggtg tggcgcgatt
     9421 ccctcggcga accagcggcg tggcagatcc accgagtgca gcggccgcgg ctcgtccagg
     9481 cggcgcacca ccagccgcga ttcgcggtag cccccgccgc tctccaccag cagctcgccg
     9541 gcgaaggcga tgccctgcgt gaacagcgtg tggtcgtgcg gcaggcgttg ctcgaccgtg
     9601 aagggcagtc ggggaatggc cgcgagcgga tcggtgcggg tgtcggcctc cccgcaggcg
     9661 accggcgcca ggcccgccag caccagcact gccgcgagcc tgcgccggca gcggcgcaaa
     9721 gccgccatca cggcaactgt cccttgatga tgcggcggat caggaagcgt gccggcgaca
     9781 gcgtgcgctg caggcgccgc gacagcggtg agggctcgcc cgcgatcgcc gccgcgatcg
     9841 cctcggcgca cagcggtgcg ctggtcaggc cgcgcgagcc gtggcccgcg ctgacgtaga
     9901 ggccacggcg atacgggccg atgcggtcga tcggcaggcg cgcgttgcgc tgcaggccgg
     9961 cgtaggcctc gcggaaagcc gcgatgtcgg gcacggggcc ggccaccggc aggcgatccg
    10021 gcgtgctggc gcgatagccc acgcgtcccg ccagcgtcgc gacgtcgatc gatgccgcat
    10081 cgaagtacgg caggatttcc tgcagccgtt gcaggttgcc gcagtgttct gcggcggtgg
    10141 gcacgaaatc gccggcgccg cgcacgaagc tggcgccgca acagatccgc tcgccgtacg
    10201 gcggcgccac gtaaccctcg gcgcagacca cgcagcgcgg cgccggcgtc agcgcggcgc
    10261 gcggcagcag cgtgatctgc ccgcgcagcg ggctcagcgg caatgcgtcg tcgcccagca
    10321 gttcgttcgc tgcggcggcg gtggcgacga tgaccgcgct ggcctgcagt gtcgcgccgg
    10381 cgtccagctg cacgcgccag cccgattcct ccgcgctgag cgcgctggcg cgccagcccg
    10441 ggcgcgtctc gatcaacggg tgatcgagtg cgcgcgcgca ggccgcgggc ggcacgatcc
    10501 agccgctcgc gggcaggtac aggccgccgt gcgcgagcgg cacaccggcc agcgccgagg
    10561 cttgcgcggc atcgaccagc cgcaccagtc gcgggtacgc ggcgaagcgc tcggcgaggc
    10621 gcgcgagcgc ggcggcgtcc tgcggcccgt gcagctgcag catcccgcag ggcgagaaac
    10681 cctcgctgtc gccggcaaac agctccgcgt agtagcgcag tgcatgcagg aaactgtgca
    10741 gcgtgaactc gccgtgcggc gtgtcggcgg cgggcagttg cgtgaacagc atgccctgcg
    10801 gattgcccga ggccccggcg gccggggccg cgtgcgcctc cagcaccgtc acgcgccagc
    10861 cacgccgtgc cagcgcgcgc gcacaggtcg cgcccgcgat gccggcgccg atcacgatcg
    10921 cgcggcgatc accctgtgat gcggcggcgg cgaggtgcca gggcgtctgc gttggcggct
    10981 gcggctcggg ggcggacttg gcgccgaaca cgccggtcag catgtcgcgc ttgcggccga
    11041 atccgggtac gcgggcgagc gtgacgccgt gcatggcgag ggcggcgcgc accgcggcgg
    11101 caacggtgaa ggtcgccagc gtggtgcccg gtttgctgag cgccgccacg cgccgcatca
    11161 ccgcgtcgtc ccacatcgcg gggttgcgcg ccggtgcgaa gccgtccagg aaccacgcgt
    11221 cggccttgac ggccagttcc ggatgcgtgt cggcgccgta ttgctccagc gcctcgcgca
    11281 cgtcgccgat atacagctgc aggctcacgc ggccagcgtc gaattccagc gtgtgcagcc
    11341 cgggcagcag cggcggccag gcgccgagca attgccgcgt gaagggatcc agctccggcc
    11401 agcgttgcag cgcgcgcgcg agcgaccctg cttcgagagg aaatgcctcg aggctgcgga
    11461 acaccagtcg cgcccgcgcc ggcgcgacgc gctgccacaa ttgccaggcg agcaggaagt
    11521 tcagcccggt gccgaagccc gtctcgtgga tcacgaacag cgtgcccggc gcatcgggca
    11581 gcgcggccca gcgcgcggcg agcctgttgt gttcgaggaa cacgtaccgc gcctcggcgc
    11641 tgccgtcgtc gcggctgtaa tagacgtcgt tgaactcgcg cgagaacggc gtgccgtccg
    11701 cgagccattc gggttcgctg gcgcgcggcg tggcaatcgg tgggggcagg tcgctggaca
    11761 tcgggcggat gataacccgg cgcaccggcg gcggcaccgc agcgcaggcc ggaacgctgc
    11821 cggctgcacc tatactgtgc cgatgatgcg cttgcggccc ctgatcctgt tgctcgcctg
    11881 cctcgcgggc ggcacctctt acgcggcgcc cggggaggcg gtcgatgccg catcgggcac
    11941 ggtgcggctg ctcctgagcg ccgagccgcc gagcctcgac tcggtgcgcg cgacggacca
    12001 ggtgagtttc ttcgtgctct cgcacctgat ggacgggctg ctgcaatacg acgccgacaa
    12061 tcgcctcgcg ccggcactgg ccgagcgctg ggagctgcgc cccgacggcg cgaccttctg
    12121 gctgcgccgc ggcgcgacct gggaggacgg cgcgcccgtc accgcgcacg acttcgtcca
    12181 tgcctggcgc gaggtgctgc gcccggcgaa cgccgcaccc tacgcctcgc tgctgtaccc
    12241 ggtgcggaac gcgcgcgcga tcgcctcggg cgagctggcg cccgagagcc ttggcgccac
    12301 tgcgccggag gaccaccgcc tcgaggtcgt attcgggcag ccctgcgcct ggttccccgc
    12361 gctcacggcc ttcatgacgc tgctgccgct gcgcgcggac ttccatgccg cccagggggg
    12421 gcgtcatgcg gccgacgcgg gcagactgct cgcgaacggc ccgttccgcc tcacccgctg
    12481 ggtgcacggc gcgcagctgg tgctggagaa gaacccgcgc tactggaatg ccggcgccat
    12541 ccgcctgcag cgcatcgagg tgccctacat caccacggat gcgcaggccg agctgaacct
    12601 gttcctggac cgccgcatcg ccatggcgaa catcgcgccg gactcggtgc gccaggtgct
    12661 gctcgagcgc cagcgtctgc gtcgcttcgc cgacggctac gtgcatttcc tcacgttcaa
    12721 tttccgcgac ggcaggccga cgcagaacct gcggctgcgc cgcgcgatcc aggcggcgct
    12781 ggatccgggc gagatcgtga accgcgtgct gcgcatgccg ggcctgcggc ccgccgaatc
    12841 gctgtttccc tcggtgctgc gtggtcgcga gcgcgcgttc gtggacgagt acccgttggc
    12901 cgcgccggcg cgcggggatg cgatcgcgca gcgcgagttc gccgccgcgc tcgccgagtt
    12961 gggcgtgacg gagctgccag cgctgcacct gctgaccggc gaggcgccca ccgcgctgcg
    13021 catcggcgaa tacgcgcagg cgcgcctcgc cgagacgctc ggcgtgcgcc tgcggctcga
    13081 tcgccagatc accaagcagc gcctgcagca gatggcgcgc ggcgacttcg acatcgcgct
    13141 gcagaactgg gggccggatt tcgacgatcc cgtcaccttc gccgaactgc tcgcctccgg
    13201 gaacccgctc aaccgcggcc agtaccgcag cgccggttac gaccgctggc tcggcgtggc
    13261 gctgggctcc acggagcagg cgcagcggct cgccgcgttc gatgcgctgc agcggctggt
    13321 gcgcgacgag gcgccgctga ttccggtcta cgagaacgcg cggctctacg tgcagcaccc
    13381 ggctttgcag ggcgtggtgc gcgcgccgtt tttcggcgat ccgaacctgc gccacgcctg
    13441 gatcgggacg cgctgaatgc ccggcgtggc tgcctgcgcg gcgttgcact atgatcgggc
    13501 gctcactggc tgccggttcc ccgcatgagc ttcccgctgc ccgatcccga gctgctgcgc
    13561 atcgtcgcgc tcgcctgcgg catgagcgtc acgctgctgg gcgcgggcca cgcgctgctc
    13621 accaagcgcg atccgcgcag cgccgccgcg tgggtgagcc tgtgcgtgtt cttccccgtg
    13681 gtcggagtgc tggcgtacct cgcgctcggc aacaaccgta tccgcacccg cgcgcggcgc
    13741 ctgcacgcgc tgccgccgca cgagcagccc gcgccgccgc cggcgcgcga acacgttgcc
    13801 gccgagcacc tgatcgcgcc gcagtaccgc aacctcgcct atctcggcct cgcggccagc
    13861 ggcaacgaac tgctgacggg caacagcgtc gaggcgctgt actgcggcga gaacgcctat
    13921 cccgcgatgc tgctcgcgat ccgcgaggcg cgccgccatg tctatctggc gacctacatc
    13981 ttcgactcgc gcggcatcgg cgccgagttc gtcgaggcgc tgggcgcggc gcaagcgcgc
    14041 ggcgtccagg tgcgcgtgct gctcgatggc ttcggcgagt tctactcgct gccgcgcgtg
    14101 ggctggcggt tgcgcgccgc cggcgtgacg acggcgcgct tcctgcagcc gcggttgtgg
    14161 ccgcctaact tcacgctcaa cctgcgcaac caccgcaaga tcctcgtggt cgacggcgag
    14221 ctcgccttca ccggcggcat gaacatccgc aacaagcaca tcgcggtgcg gggcgtgcgc
    14281 ccggccgatg tgatcgatgt gcactttcgc ctgcgcggac cgatcgtcac gcagatcgag
    14341 gaggtgttcg cggccgactg gctgttcagc acgcgccagg cgctgccccc gcccccgagc
    14401 gtggcgccgg tgacgatgct gccggaggag gccgagtgcc gcgtgatcgt cgacggcccg
    14461 aacgaggatc tcgacaagct gctgtgggtg ctggtggggg cgatctcggc ggctcgatcc
    14521 agcatccgga tcatgacgcc gtacttcctg ccgccgcccg agctcgccgt ggcgctgcag
    14581 acggccgcgc tgcgtggcgt cgaggtgacg atcgtgctgc cggcgcacaa caatttccct
    14641 ttcatgacct gggccacgac ccacatgctc ggcgagctgc tgaagaccgg ggtgcgggtg
    14701 catttctaca ccggtccctt cctgcacacc aagctgctgc tggtcgatga ctactacgca
    14761 cagatcggct cctcgaacct ggacccgcgc agcctgcggc tcaatttcga gattgcggtc
    14821 gagatctacg accacgtctt cgggcgcggc gtggcggtgc atttcgccga gatgctcgcc
    14881 acatcgcgcg agctgtcctt ctctacctgg caggagcgca gcctgccgcg gcgcctgcgc
    14941 gatgccgtgt tctgggtgtt ctcgccctac ctgtagccgc ggcggggcgg atcggctaac
    15001 gtaggcgccc ccgaacagac ccccggagag ggaccagtcc gacatgagcc agagccagtc
    15061 cgtcagcatg gaatcgcaac ggtttttcac aggggcactg aacgtgctcc atgcggggtt
    15121 catcagggcg tcgcgggcgc aggcgaaacg ccactacgcg cgcgtgcacg gtggcggggt
    15181 gctggagctg gcgaagctga agctgggtga cggttcggac ctgctgttcc gcgtggcgct
    15241 cgaccagagc gagttccgtg gcaagctcga cttcgcgacc ttccgcaagg ccctggtgca
    15301 gttgcttgcc aagctgcagg accgcatccg cttcaagggc gagctgaaca tctaccgcaa
    15361 cgatgaaacc ggcgcgatgc tgctgaacct gccctcgatc ctcaccgagg acgggcgcac
    15421 caacgtgatg atgctcggcg tggacccgcc cgaaccgggc gtggtcacgc tgcgcctgca
    15481 gttcctcgac ccggagcaat tcaggcagcc ggcagcgacg gcctagcttc tgcgcaggtc
    15541 cgcattcatg cggacattgt ttccactgta gatgcggctg aagagggtgt cgcaaaagcc
    15601 attacgttgc gagcgagcgg cttggtgcgt cgctggctgt gctgcccgcc attgtcct
//
LOCUS       DAORUQ010000064        15478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3579_length_15478_cov_7.812293, whole genome shotgun sequence.
ACCESSION   DAORUQ010000064 DAORUQ010000000
VERSION     DAORUQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3579_length_15478_cov_7.812293"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLW13_07515"
     CDS             <1..644
                     /locus_tag="PLW13_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HQY70258.1"
                     /translation="YYVASAGECMRIESLFVTQVYAARLQHARSRLNARLLLECLQLR
                     HDDAAGRRWSAQNYAGGYTSYGSASRMHERSPTFAALARRIDRHVRAYARALAFDIDV
                     RRLAMTDCWINIMPRGVAHGAHLHPLSLVSGTYYVRTPSGSAGLKFEDPRLERFMAAP
                     PRRRTARRELRCWVGVPARAGRVVLFESWLRHEVPAGRTASERVSISFNYGLR"
     gene            650..1831
                     /locus_tag="PLW13_07520"
     CDS             650..1831
                     /locus_tag="PLW13_07520"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQY70259.1"
                     /translation="MSEGPRFVPFAYGFRPFFLGAGLFAVIGIAAWVWIYVSGSTPLG
                     ELPPFLWHGHEMLYGFICAAIAGFLLTAVPSWTGSRGFAGTPLVILTLLWLAGRVAFA
                     TAAALPPLWLAVAELSFLPMLAFLIGRSLLRERNRNFPMLIIVAVLWGIDAWFLYAIT
                     TGDFTLASRALRVGIGVVALLITVIGGRVVPNFTASALRRRGIAVEIRSRVLVERVTI
                     ASMALAVAIDAVAPWHWSAAAVAGVAAIAHAVRLSGWRSLRTLAEPIVWVLHAAYVWL
                     PIGLALKAIHLGTGAAWAFQWQHALTVGAAATMILGVTTRAALGHTGRPLIVSRGIGI
                     AYVLLLLAALVRVFGPALLPASYIATIETAAILWIVAFTIYLVIYAPILTLARVDQKP
                     G"
     gene            complement(1833..2750)
                     /locus_tag="PLW13_07525"
     CDS             complement(1833..2750)
                     /locus_tag="PLW13_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY70260.1"
                     /translation="MSPAKRLNYQHLYYFWTVVRAGSLTKAAGELNLSVPAVSAQLRT
                     LETRLGEKLLVKAGRRLVPTDTGRTAFRYAEDIFRLGRELVDALAPGPSARPLRLAIG
                     IDDVVPKELAQRLIAPALVLGRRVHLACHEGTLERLTAGLAVQDLDVVLSDAPVPAGL
                     GIRAHNHRLGGSSVSWMGSRALVKRLQARFPASLAGAPVLLPTEDTAIRRALDQWFER
                     QNLRPLVIGEFEDYALLREFARAGNGLIPVPDVIGGQFRDTSELAVLGSARPVEAEFF
                     AVTLRREIDHPAVAAICQRASALFARPDA"
     gene            2847..3320
                     /gene="rnk"
                     /locus_tag="PLW13_07530"
     CDS             2847..3320
                     /gene="rnk"
                     /locus_tag="PLW13_07530"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQY70261.1"
                     /translation="MRQNPILVSESDADRLRALLGARDDSAHDQAHLHELEAELERAI
                     VRLADEVPPDVITMQARVRVRDLATGAEEEYTLVLPAEANIAARRISVLAPLGTALLG
                     YREGDEVEWRMPGGLRRLRILKVTRELSDPAAAAPAEPRGAGRSSTPRAPLPAAS"
     gene            complement(3232..3690)
                     /locus_tag="PLW13_07535"
     CDS             complement(3232..3690)
                     /locus_tag="PLW13_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQY70262.1"
                     /translation="MDGVDRRLLAALQRDGRATNAALAAHLGLSESACLRRTRRLEEA
                     GVIRGYGARIDAGTVGLTVRVLVRITLKSQTDRDFNAFERAVQAVPNVLDCFLTTGQW
                     DYELIAIARDAADLERLHSTVLTKLPGVARVESSFVLRHVVRQVPLPLDR"
     gene            3796..4878
                     /gene="ald"
                     /locus_tag="PLW13_07540"
     CDS             3796..4878
                     /gene="ald"
                     /locus_tag="PLW13_07540"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQY70263.1"
                     /translation="MKIGVPREIKNHEYRVGLVPEAVRGLAAAGHEVTVETGAGTAIG
                     FSDQDYAAAGARVAAGAEETFRGAELIVKVKEPQPEECRRLTRDHVLFTYLHLAADRA
                     QAEALMASGCTAIAYETVVGPDGSLPLLMPMSEVAGRMSVQVGAQYCTKAFGGRGVLL
                     SGVPGVAPARVVVIGGGVSGSHAAEMAVGLRADVTIFERSVPRLRELDQLFAGRARVL
                     FSSPQAIGAALEGADLLIGAVLIPGAAAPRVVTRAMLRRMGAGAVAVDIAIDQGGCLE
                     TSRPTSHANPTYVDEGVIHYCVTNMPGAVPRTSTFALNNVTVPYVHQLAAHGWQEATA
                     RDPGLREGVNVTAGRIVHPAVQRALQ"
     gene            4942..5553
                     /locus_tag="PLW13_07545"
     CDS             4942..5553
                     /locus_tag="PLW13_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQY70264.1"
                     /translation="MGLLFPQLPFGFDDLEPAMSQDALSLHFTRHHLDHFERASALAR
                     RVGLDHLTLDELVVSASRASRLHTLYRHSAEAWNHACFWRSMRAGGGGAAYGAIGDCL
                     RQRFGSHERFARRFHGVAATLFGNGWLWLTWKGDRIRLLTTGAGDTPLVRGHIVLLAV
                     DLWEHAYYLDHQNRRGAWLRTFLDELVDWDRVNRVLEGREESA"
     gene            5550..5912
                     /locus_tag="PLW13_07550"
     CDS             5550..5912
                     /locus_tag="PLW13_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HQY70265.1"
                     /translation="MIRAKRVYEDPARGDGRRFLVERLWPRGMKKEALAAEAWLKDVA
                     PSAALRQWYGHRVERWQEFRRRYREELTVHPETWATLMSAARRGNVTLLYSAHDVEHN
                     SAKVLGDFLTARISRSQR"
     gene            5909..6859
                     /locus_tag="PLW13_07555"
     CDS             5909..6859
                     /locus_tag="PLW13_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HQY70266.1"
                     /translation="MKLATRGPFDLEATVRVLQRRPTNRVDVWTDGRYRRVFETSRGL
                     ALAEVVDRGSTDAPDLRLLVRDARGNAAVRREVARTLRRLLGLDIDPAPLHARAAGEP
                     AIRAAAHALRGLRPPRFASLFEAFASVVPFQQVSLDAGVAVVGRVVEKFGAWVEHGGE
                     RHRAFPTAVAIAAARPAALRACGLSAQKAGTLQAVAAVISRGELHEEQLEALTTADAI
                     ARLTALKGVGPWTAGLVLLRGCGRLDVFPPGDVGADRNLRALLGTQDERKVRAAIARF
                     GDLRGYLYFCALGGSLIARGLLTVSGDSWTRVNRDAHSPF"
     gene            6937..8631
                     /locus_tag="PLW13_07560"
     CDS             6937..8631
                     /locus_tag="PLW13_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQY70267.1"
                     /translation="MAAGLLATACGGSGSPGGDNPPPPPPPPPAGVSKTEAFAFLNQA
                     TFGATEAEAQRLQSLGDASNAYARWIDAQLQQPVSLQLPHVQAVYATLPQPVQNIGRL
                     NNDRVDIWFRNVVNGPDQLRQRVAFALSEIMVVSQQSALQNTPYALADYYDLLARDAF
                     GDFRQLMEDVTLHPAMGVYLSMLGNQKPDAARNIRPDENYARELMQLFTIGLVQLNLD
                     GTPATDSEGQTVPVYDQSVIEGFAHVYTGWNWAGAASFGQARPSLQNQVQPMQAYAVQ
                     HDPGAKKLLSYAGAALVGIPARNPADPARDLDDALDNIFHHPNVAPFISKQLIQRLVT
                     SNPSPAYVQRVAQVFNNDGTGRRGNLAAVVRAVLLDAQARSAPDATTAGKLKEPLLRV
                     TQLWRAYGARSQSGRYNVQSPAAVAGQGPLQAPSVFNFFSPFYAPPGEVADRDLVAPE
                     MQITTEYLATLLTNYFFVQTFCYTSAPVTGCPAVRPEVRTDLVFIDVSAEMALANDPA
                     ALVTRIAGKLLGGEISGTLAAQARAAIERAPASLGGLKVAEALYLIASSPEFAAQR"
     gene            8643..10052
                     /locus_tag="PLW13_07565"
     CDS             8643..10052
                     /locus_tag="PLW13_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQY70268.1"
                     /translation="MNRKLFVKRRQLLKLAAAGGMAYAFGRLPDAVYAQAVNAYARFA
                     DYKALVCVFLFGGNDSWNMVVPRSAAEYQAYQLARQNLAVDQASLLPITPVAPLPNGA
                     AIGLHPSMPEIATLFEQDAACALIANVGPLITSTSLAQYQARSVPLPPQLFSHNDQQD
                     QWHSLRGLAQANTGWAGRISDALGTQTGPAIPLNVSLSGQTLFQAGESAGSYVIGPTG
                     PVEFFGLDGTDALSVARKSGFEAVARAAYGTLYERAYAGVQQRALDSAQAVNAALATV
                     RAPNPVPFATPFPPSPLGTQLKTVAEMIAVRGTLQASRQIFFVATGGFDSHDDQAQDQ
                     PGLLANVSACLDAFYRATVEMGIAQSVTAFTQSDFGRTLTSNGDGTDHAWGGVQLVVG
                     GAVHGKSVYGSYPVLRLGATLAADGADDVGGGRFIPTTSSDQYAATLARWFGVLDADL
                     ARIAPGIDNFTQRDLGFMS"
     gene            10076..12958
                     /locus_tag="PLW13_07570"
     CDS             10076..12958
                     /locus_tag="PLW13_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019829083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HQY70269.1"
                     /translation="MEHSPSLALIVLLPFLGSLCAAFLPSNARNAESWLAGGIALACT
                     VLTLGLYGRVASGAVLHFVLPWLPQAGLDFSLRLDGFAWLFTLLVSLMGTLVVIYARY
                     YLSPQDPVPRFFSFLLAFMGSMLGIVLSGNLIQLVVFWELTSFSSFMLIAYWYHRAEA
                     RQGARMALTVTATGGFCLLAGVIMLGTVAGSFDLDRVLAAGDTVRAHPWYPVMLGLIL
                     LGALTKSAQFPFHFWLPHAMAAPTPVSAYLHSATLVKAGVFLLARLWPVLAGTDLWFA
                     LVCGAGLASLLLGAAAALFQRDMKGVLAYSTVSHLGLITLLLGMNSPLALVAAVFHIM
                     NHATFKATLFMAAGIVDHEAGTRDLRRLNGLARAMPITATIATVAAAAMAGVPLLNGF
                     LSKEMFFAEAIFAGEEPLLRVGLPALATLAGIFSVAYSLRLVHQVFFGAPARELPRTP
                     HEPTRWMLLPGALLVLTCLLVGILPARTIGPALALAVQSILGAATPAYSLVVWHGFTA
                     PFLMSVIALVGGVGLYLLRYFYGQRATGRAPLRAPFDGRRVFDILIVVLHRAAERMVR
                     FASSRRQQPQLLLIVCLALAVAAIPLAASGLSGGGAPLTPIDPAYVLPWFVGCLCAIG
                     AAVQAKFHRLAALIMLGGAGLFTSLTFAWFSAPDLALTQIAVEVVTLVLLLLGLRWLP
                     RRLELDDPDRRTRRARARRVRDFLLALVVGGSLAALSFAMLTRAPVNGIAHFFIGRAL
                     PEGGGRNVVNVILVDFRGFDTLGEITVVAIVALSVYALLRRFRPALESIEVPRAQREA
                     LAHGKRPVFDPDAKLPAGDLAVPAVLVRLLLPMAGLVAVYLLLRGHDAPGGGFVGGLV
                     MATASITQYMVGGAQWVEWRMRIHPQYWVALGLLAAGGAGVLAWSIGQPFLTSFGAEL
                     ALPVVGDVHVASVLLFDLGVYLVVVGATSLMLVALAHQSFRSQRGA"
     gene            12961..13296
                     /locus_tag="PLW13_07575"
     CDS             12961..13296
                     /locus_tag="PLW13_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HQY70270.1"
                     /translation="MEILFAIAIGVLSGSGIWLLLRPRTFQVIVGLALLSQAVNLFIF
                     GMGRLATGKAPILGAHPVDPASLADPVPQALVLTAIVIGFATTALLLVVLLAARGLTG
                     TDHVDGRES"
     gene            13296..14969
                     /locus_tag="PLW13_07580"
     CDS             13296..14969
                     /locus_tag="PLW13_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HQY70271.1"
                     /translation="MSSWFRHLPVVPIVLPLLAAALILLLAERARALRIAIACVSITV
                     QLVAAIMLLALTTEAFPDIWPQGVGAYAIGSWPAPFGIVLVIDRLTGLMLTLTAVVGF
                     AVFINSLAYWDRVGPHFHALLQLLLMGVNGAFLTGDLFNLFVFFEVLLAASYGLLLHG
                     SNSQRVKMGLHYIVVNLVGSLLFLIGVSLIYGVTGTLNMADLPARAAALSDADRALFD
                     AAAAVLGIAFLTKAGCWPLNFWLPGTYAAASPPVAAAFAILTKVGVYALLRLGSLIGS
                     ANALAHIEGSALLRPDLFRGGMFYIGIMTMGFGIFGTLAAQQLARVVGFSVIVSSGLL
                     LTIMGLDHTPLLAPGLFYLVGSVLATSAFFMLTGMTGRTWRTPTVPATVPATPVSVPT
                     SGYIPFGVRLAAAPHGEDDEVGVAIPAAMAFMGLMFAFCVLLVTGSPPLSGFVAKFAI
                     LAAAIQAAQGAPWAAAAWMLVGLTIAAGLASLIALTRIGMRLFWSVTGRSTPRLRALE
                     AGPVAMLVFLCIALTVAAGPAMGFLEDTAASLRAPRIYIDTVLATGEGR"
     gene            14978..15454
                     /locus_tag="PLW13_07585"
     CDS             14978..15454
                     /locus_tag="PLW13_07585"
                     /inference="COORDINATES: protein motif:HMM:NF006520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HQY70272.1"
                     /translation="MPVLGGALVAMWLALNDTVAPGQVLLGALIAVALILWAAKLRPL
                     RPRLYRPWLAISLLAVVFVDIVRSNIAVGRIILGLVPVRAIRSGLLEIPLDLHDPHGL
                     AVLATIITSTPGTVWVEHVAERRTLTLHILDLKDEAGWIRDIKDRYERPLIRIFQP"
BASE COUNT         2200 a         5207 c         5185 g         2886 t
ORIGIN      
        1 gatactacgt ggcttctgcc ggtgagtgca tgcgcatcga atccctgttc gtcacacaag
       61 tttatgcagc gcggctgcaa cacgcgcgca gccggctcaa tgcacggttg ttgctggaat
      121 gcctgcagtt gcgccacgac gatgccgcgg gccgccgctg gtcggcgcag aactacgccg
      181 gcgggtatac ctcttacggc tcggcgagcc gcatgcacga gcgctcgccg accttcgcag
      241 ccctggcgcg ccgcatcgat cgacacgtgc gggcctacgc gcgtgccctc gcgttcgaca
      301 tcgacgtgcg caggctcgcg atgacggact gctggatcaa catcatgccg cgtggcgtcg
      361 cgcatggcgc gcatctgcat ccgctatcgc tcgtgagcgg cacctactac gtacgtaccc
      421 cgtcgggctc ggcagggctc aagttcgagg acccgcgcct cgagcgcttc atggccgcgc
      481 caccgcgacg gcgtaccgcc cgccgcgaac tgcggtgctg ggtgggggtg ccggcaagag
      541 cgggcagggt ggtgctcttt gaaagctggc tgcgacatga ggtcccggcc ggtcgcacgg
      601 catccgaacg tgtcagtatc agtttcaact atggtctcag gtgagcacga tgagtgaagg
      661 tccccgcttc gtccccttcg catatgggtt tcgccccttc tttctcggtg ccgggctctt
      721 cgccgtgatc gggatcgccg catgggtctg gatctacgtc agcgggagca cgccgctcgg
      781 cgaactgccg ccgtttctgt ggcacggcca cgaaatgctt tacggcttca tctgcgccgc
      841 gatcgcgggc ttcctgctca ctgccgtgcc cagctggaca ggttcacgcg ggtttgccgg
      901 cacaccgctc gtgatcctga cgctgctgtg gctcgcgggg cgcgtcgcgt ttgcgacggc
      961 ggccgcactg ccgccgctgt ggcttgccgt tgcagagctc tcgttcctgc cgatgctcgc
     1021 cttcctgatc gggcgctcgc tgctgcgcga acgcaatcgc aactttccga tgctgatcat
     1081 cgtcgcagtg ctgtggggga tcgacgcgtg gttcctctat gcgatcacga ccggagactt
     1141 cacgctcgcg agccgcgcgt tgcgggtcgg catcggtgtc gtcgcgctcc tcatcaccgt
     1201 gatcggcggg cgcgtcgtgc cgaacttcac ggcgagcgcg ctgcgtcgtc gcggtattgc
     1261 cgtcgagatc aggagccgcg tcctggtcga gcgtgtgacg atcgcatcga tggcgctcgc
     1321 ggtcgcgatc gacgccgtgg caccctggca ctggagtgct gcggcggtgg caggcgtcgc
     1381 ggcgattgcg catgccgtgc gcctgtccgg ctggcggagc ctgcggaccc ttgccgagcc
     1441 catcgtatgg gtgctgcacg cggcctatgt gtggctgccg atcgggcttg ctctcaaggc
     1501 gatccacctc ggcaccggtg ctgcctgggc gtttcaatgg cagcacgcgc tcacggtcgg
     1561 tgccgcggcc acgatgattc tcggggttac cacacgcgcg gcgctcggtc acaccgggcg
     1621 cccgctcatc gtgtcgcgcg ggatcggcat cgcctatgtc ctgctgctgc tcgcggcgct
     1681 ggtgcgcgta ttcggacccg cgttgctgcc cgccagctac attgcgacga tcgagacggc
     1741 ggcgatcctg tggatcgtcg cattcaccat ctatctcgtg atctacgcac cgatcctcac
     1801 gctggcgcgc gtggatcaga agccggggta gctcaggcgt ccgggcgcgc gaagagtgcg
     1861 ctcgcgcgct ggcagattgc cgccaccgcc ggatggtcga tctcccggcg cagcgtcacg
     1921 gcgaagaact ctgcttcgac gggcctcgcg gagccgagca cggcgagctc tgaggtatcc
     1981 cggaactgcc cgccgatcac gtcgggtacc gggatgagcc cgttgcccgc gcgcgcgaat
     2041 tcccgcagca gcgcatagtc ctcgaattcc ccgatcacga gtgggcgcag attctgtcgc
     2101 tcgaaccact ggtcgagggc gcggcggatc gctgtatctt cagtcgggag gagcaccgga
     2161 gcgccggcga gcgatgccgg gaagcgtgcc tgcaggcgct tcacgagcgc gcggcttccc
     2221 atccacgata cgctgctgcc gccaagcctg tggttgtgcg cgcggatgcc gaggccggca
     2281 ggcactgggg catctgacag cacgacgtcg agatcctgta cggcgagtcc tgcagtgagt
     2341 cgctcgagcg tgccttcgtg acaggcaagg tgtacccggc gcccgagcac gagggccggg
     2401 gcgatcagcc gctgcgcaag ctccttcggc acgacgtcgt cgatgccgat tgcgagacgc
     2461 agcggacgcg cagagggccc gggcgcaagc gcatccacca gttcgcggcc cagccggaag
     2521 atgtcttctg cgtagcgaaa ggcagtgcga ccggtgtcgg tggggacgag gcgccggcct
     2581 gccttcacca gcagcttctc gccgagacgc gtctcgagcg tgcgcagttg cgcactgact
     2641 gccggaacgg agaggttcag ctcacccgcc gctttggtga gactgccggc acgcacaacg
     2701 gtccagaaat aatacaggtg ttggtagttg aggcgttttg cggggctcat gacacttcca
     2761 gaaaatgaaa cacagcgtca agttaattat aatggattta actgacggat tcagctaaac
     2821 agggactcaa tcctttggag ctggacatga gacagaaccc gatcctcgtg agtgagtcgg
     2881 atgccgatcg gctgcgcgcg ttgctcggtg cccgggatga ctctgcgcac gaccaggccc
     2941 acctgcacga actcgaggcc gaactcgagc gtgcgatcgt gcgcctcgcg gacgaagtgc
     3001 cgcccgacgt gatcacgatg caggcgcgtg tgcgcgtgcg cgatctcgcg accggcgccg
     3061 aagaggagta cacgctcgtg ctgccggcgg aagccaatat cgcggcgcgg cgcatctcgg
     3121 tactcgcacc actcggcacg gcgctgctcg gttaccgcga gggtgatgaa gtcgaatggc
     3181 gcatgcccgg cgggctgcgg cgcctgcgca tcctcaaggt cacgcgcgaa ctcagcgatc
     3241 cagcggcagc ggcacctgcc gaaccacgtg gcgcaggacg aagctcgact ccacgcgcgc
     3301 cactcccggc agcttcgtga gcaccgtgct gtgcagccgc tcgaggtcgg ccgcatcgcg
     3361 ggcgatcgcg atcagctcgt agtcccattg cccggtcgtg aggaagcagt cgagcacgtt
     3421 tggcacggcc tgcaccgcac gctcgaacgc gttgaaatcg cggtcggtct ggctcttgag
     3481 cgtgatgcgc acgagcacac gcacggtgag gcctacagtg cccgcgtcga tgcgggcgcc
     3541 gtagccgcgg atgacgcccg cttcctcgag gcggcgtgtg cggcgcaggc aggcggactc
     3601 cgacaggccg aggtgagcgg ccagcgccgc attcgtcgcc cggccatcgc gctgtagcgc
     3661 cgcaagaagc cgtcgatcaa cgccgtccat gcgcaagatt atggcgcatg cggcgctcta
     3721 ctgtcttgta agtggcagcg cctgcggcgg gtccggttgc agaatgcgac tccacatccc
     3781 cgggggagtc gggccatgaa gatcggcgtg ccgcgcgaaa tcaagaatca cgagtaccgc
     3841 gtcggactcg tccccgaggc cgtcagaggg ctcgcggcgg ccgggcacga agtcacggtg
     3901 gaaacgggtg ccggcaccgc gatcggcttc agcgatcagg actatgctgc cgcgggcgcg
     3961 cgggtcgcgg cgggcgccga agaaaccttc cgcggcgcgg agctgatcgt caaagtgaag
     4021 gagccgcagc ccgaggagtg ccggcgcctg acgcgtgacc atgtgctctt cacgtacctg
     4081 caccttgccg ccgatcgtgc gcaggcggag gcgttgatgg cctccggctg tacggcgatc
     4141 gcctatgaaa cggtcgtcgg cccggatggc tcgctgccgt tactcatgcc gatgagcgaa
     4201 gtcgccggac gcatgtccgt gcaggtcggc gcgcagtatt gcaccaaggc gttcggtggc
     4261 cggggtgtgc tgctcagcgg ggttcccggt gttgcgccgg cgcgcgtggt cgtgatcggc
     4321 ggtggtgtat cgggcagtca cgcggccgaa atggccgtgg gcctgcgtgc cgatgtcacg
     4381 atcttcgagc gttccgtgcc gcgcctgcgc gaactcgatc agctctttgc cgggcgcgcg
     4441 cgcgtgctgt tttcctcgcc ccaggcaatc ggggcagccc ttgagggcgc ggacctgctg
     4501 ataggagctg tgctgattcc cggcgcagcg gcgccgcggg tcgtcacccg tgcgatgttg
     4561 cggcggatgg gagccggcgc ggtcgcggtc gatatcgcga tcgatcaggg cggctgtctc
     4621 gagacgagcc gcccgacctc gcacgccaac ccgacctacg tagacgaagg cgtaatccac
     4681 tattgcgtga caaacatgcc cggcgcggtg ccacgcacgt cgacctttgc gctgaacaac
     4741 gtgacggtgc cctatgtgca ccagctcgca gcccacggct ggcaggaagc caccgcgcgc
     4801 gatccgggtt tgcgcgaggg cgtgaatgtc accgccggtc gcatcgtgca cccggccgtg
     4861 cagcgcgcat tgcagtgatg taatgcgcgt acgtcccgtc gccagtgcgg aacgatcaat
     4921 gccgctacgg gtcgaatggg catgggtctc ttgtttccgc agctgccgtt cggattcgac
     4981 gatcttgagc cggccatgtc gcaggacgct ttgtcgttgc acttcacgcg gcatcacctc
     5041 gatcatttcg aacgcgcctc ggcgctcgcg cgtcgcgtcg gacttgatca cctgacgctc
     5101 gatgaactcg tcgtcagcgc aagtcgcgcg tcgcgcctgc acactctcta tcgacactcg
     5161 gcggaagcct ggaaccacgc ctgtttctgg cggtcgatgc gcgcgggtgg cggtggagcc
     5221 gcttacggtg ccatcggcga ttgccttcgc cagcgtttcg gcagccacga gcgcttcgcg
     5281 cgccggtttc atggcgtagc ggccaccctg ttcggcaacg gctggctctg gctcacctgg
     5341 aaaggggatc gcatccggct gctcacgacg ggcgcaggtg acaccccgct cgtgcggggt
     5401 cacatcgtgc tgctcgcggt cgacttgtgg gaacatgctt actatctcga tcatcagaac
     5461 cgccgcggtg cctggctgcg tacgtttctg gacgaactgg tcgactggga cagggtcaac
     5521 agggtgctcg aaggcagaga ggagtccgca tgattcgcgc caaacgtgtc tacgaggacc
     5581 ccgcgcgcgg tgacggacgc cgctttctgg tcgagcgcct gtggccccgg ggcatgaaga
     5641 aggaggcgct cgctgcagag gcctggctga aggatgtcgc gccgagcgca gcgctgcggc
     5701 agtggtacgg gcaccgcgtc gagcgctggc aggagtttcg ccgccgctac cgcgaggagc
     5761 tcacggtgca tccggagacg tgggcaaccc tgatgtccgc cgcgcgacgc ggcaatgtca
     5821 cgctgctcta cagcgcgcac gacgtcgagc acaacagcgc aaaggtgctg ggcgattttc
     5881 tcacggcgag gatcagccgg tcgcagcgat gaagctcgcg actcgcgggc cgtttgatct
     5941 cgaggcgacc gttcgggtgt tgcagcgccg gccgacgaac cgggtcgatg tgtggacgga
     6001 tggtcgctat cgacgggtgt tcgaaacatc ccgcggcctt gcgctggccg aggtcgtcga
     6061 tcgcggcagc accgacgcgc cagacctgcg cttgctcgtg cgtgacgcca gggggaacgc
     6121 cgcggtgcgc cgtgaagtcg cgcgcacgct gcggcgtctg ctgggactcg acatcgatcc
     6181 cgcgccgttg cacgcgcggg cggcaggcga gccggcgatc agggccgcag cgcacgcgct
     6241 gcgcggcctg cgacctccgc gtttcgcgag cctcttcgag gcgtttgcga gtgtcgttcc
     6301 gttccagcag gtgagcctcg acgctggcgt ggctgtggtt ggacgcgtgg tcgagaagtt
     6361 cggtgcgtgg gtggagcacg gcggcgaacg ccatcgggcc tttccaactg ccgtggcgat
     6421 cgccgctgcg cggccggcag cgctgcgtgc ctgcgggctc tccgcacaga aggcaggcac
     6481 gctgcaggcg gtggcggcgg tgatttcgcg tggcgagctg cacgaagagc agctcgaggc
     6541 gctcacgacc gctgatgcga tcgcacgcct cacggcgctc aagggcgtgg gcccctggac
     6601 ggccgggctc gtattgctgc gtggctgcgg ccggctcgac gtgtttccac caggcgatgt
     6661 gggtgcggat cgcaacctgc gcgcactgct tggcacgcag gatgagcgca aggtccgggc
     6721 tgcgatcgcc cgcttcggag atctccgtgg ctacctctat ttctgcgcgc tcggcggcag
     6781 tctcattgcg aggggcctgc tgacggtctc tggcgacagc tggacgcgtg tcaaccgcga
     6841 cgcccatagt ccgttctgag gttcggacga ctcgttcgca gggagaatgc catgggctcg
     6901 ttgttgctcg gatcgcggcc gcggctcgcg ctggtcatgg cggccggcct gctcgccacc
     6961 gcctgcgggg gctcaggcag tccaggtggc gacaatcctc caccaccccc accgccccca
     7021 ccggcgggcg tcagcaagac agaggccttc gcgttcctca atcaggcgac cttcggcgcg
     7081 accgaagccg aggcccagcg tctgcagtcg ctcggcgatg cgagcaacgc ctacgcgcgc
     7141 tggatcgatg cacagctgca gcagcccgtc tcgctgcaac tgccacacgt gcaggcggtg
     7201 tatgccacgt tgccgcagcc cgtgcagaac atcggccgcc tgaacaacga tcgcgtcgac
     7261 atctggtttc gcaatgtcgt caatggtccg gaccagctcc gccaacgcgt ggctttcgcg
     7321 ctctccgaga tcatggtggt ctcgcagcaa agcgcgctgc agaacacccc gtatgcgctc
     7381 gccgactatt acgacctgct cgcccgcgac gcattcggtg attttcgtca gctcatggag
     7441 gacgtgaccc tgcatccggc gatgggcgtc tacctctcga tgctcggcaa ccagaagccc
     7501 gatgccgcac gcaacatccg ccccgacgag aactacgcgc gcgagctgat gcagctgttc
     7561 accatcggac tcgtgcagct caacctcgac ggcacacccg ccacggatag cgagggacag
     7621 acggttcccg tttacgatca atccgtcatc gagggatttg cgcacgttta caccggctgg
     7681 aactgggcgg gcgccgcaag ctttggccag gcgcgcccgt cgctgcagaa ccaggtgcag
     7741 ccgatgcaag cctatgccgt tcagcacgac cccggggcga agaagctgct gagctatgcg
     7801 ggcgcggcac tcgtcgggat tccggcgcgc aatccggcgg accccgcgcg ggatctcgac
     7861 gatgcgctcg acaacatctt tcaccatccg aacgtcgcgc ccttcatctc taaacagctc
     7921 atacagcggc tcgtgacgag caatccctcg ccggcttatg tccagcgcgt cgcgcaggtg
     7981 ttcaacaacg acggcacggg gcgcaggggc aatctcgccg ccgtggtgcg ggccgttctg
     8041 ctcgacgcgc aagcgcgcag cgctccggat gcaacaactg cgggcaagct gaaggagccg
     8101 ttgctgcgcg tgacccagct ctggcgcgcg tacggggcgc gctctcaaag cggccgctac
     8161 aacgtgcaga gtcctgccgc cgtggcgggg caggggccgc tgcaggcacc ttccgtgttc
     8221 aatttcttca gtcctttcta cgcgccaccc ggcgaggtgg ccgatcggga tctcgtcgcg
     8281 cccgagatgc agatcacgac cgagtatctc gcgacgctgc tcacgaatta ctttttcgta
     8341 cagaccttct gctacacctc ggcgccggtc accggttgcc ccgccgtgcg gcccgaagtt
     8401 cgcaccgatc tcgtgttcat cgatgtgagc gctgaaatgg cgctcgccaa cgacccggcg
     8461 gccctggtga cacgcattgc cggcaagctg ctcggtggag agatctccgg cacgctcgcg
     8521 gcacaggccc gcgctgccat cgagcgtgca ccggcgagcc tcggcggact gaaagtcgcc
     8581 gaagccttgt atctcatcgc gagctcgccc gagttcgcag cgcagcgctg aaggggctcc
     8641 ccatgaaccg caaactcttc gtgaagcgtc gccagctcct gaaactcgcg gctgcgggcg
     8701 ggatggctta tgccttcggc cggctgccgg atgccgtgta cgcgcaggcc gtcaatgcgt
     8761 acgcacgatt cgccgactac aaggcgctgg tgtgcgtgtt cctcttcggc ggcaacgact
     8821 cgtggaacat ggtcgtgccg cgcagcgcgg ccgagtacca ggcctaccag ctcgcgcgcc
     8881 agaatctcgc agtcgaccag gcgagcctgc tgccgatcac gccggtcgca ccgctgccga
     8941 acggcgccgc gatcggcctg catccatcga tgccggaaat cgcgacactc ttcgagcagg
     9001 acgccgcctg tgccctcatc gcgaacgtgg ggccgctcat cacgtccact tcgctcgcgc
     9061 aatatcaggc acgctccgtg ccgctgccgc cacagctctt ctcccacaat gatcagcagg
     9121 atcagtggca ctcgctgcgt ggacttgcgc aggcgaatac tggctgggcc ggacgcatct
     9181 ccgatgcgct cggcacgcag accggaccgg cgattccgct caatgtgtcg ctctcggggc
     9241 agactctctt tcaggccggg gagagcgcgg gttcctacgt catcggcccg acgggcccgg
     9301 tcgagttctt tggccttgac ggcacagatg cgctttccgt cgcgcgcaag agcggtttcg
     9361 aggcggtcgc acgcgccgcc tacggcaccc tttacgagcg tgcgtatgcg ggagtgcagc
     9421 aacgcgcgct cgattccgcc caggcggtca atgcggcgct cgcgaccgtg cgagcaccga
     9481 atcccgtgcc gttcgccaca ccctttccgc catcgccact cggcacgcaa ttgaagaccg
     9541 tcgccgagat gatcgcggtg cggggcacgt tgcaggcctc acgccagatc ttcttcgtcg
     9601 ccacgggcgg cttcgattcc cacgacgatc aggcgcagga ccagccggga ttgctcgcga
     9661 acgtgagcgc gtgcctcgat gcgttctatc gcgcaacggt cgagatgggc attgcgcaga
     9721 gtgtcaccgc attcacccag tcggatttcg ggcgcaccct gacttccaac ggtgatggta
     9781 ccgatcacgc ctggggtggc gtgcaactcg tcgtcggtgg cgcggtgcat ggcaagagtg
     9841 tctacggcag ctatccggta ctgcgcctcg gcgcgaccct cgcagcggat ggcgccgacg
     9901 atgtcggcgg tggacgcttc attccgacga cttcctccga ccagtatgca gcgacgctgg
     9961 cgcgctggtt cggtgtgctc gacgcggatc tggcgcgcat cgcgcccggc atcgacaact
    10021 tcacgcagcg tgatctcggc ttcatgagct gacgtgtgct acaagtgcgc gcgacatgga
    10081 gcattctccg tcgctcgcgc tgatcgtcct gctgccgttc cttggcagtc tttgtgctgc
    10141 attcctgccc tccaatgccc gcaatgccga gtcgtggctc gcgggcggga tcgcgcttgc
    10201 gtgcacggtg ctcaccctcg gcctctacgg ccgggtggca agtggcgccg tcctgcactt
    10261 cgtgttgccc tggctgcccc aggcaggcct cgacttcagc ctgagactcg acgggttcgc
    10321 ctggctcttc acgctgctcg tgtcgctgat gggtacgctg gtcgtgattt acgcgcgcta
    10381 ctacctgtcg ccgcaggatc cggtgccgcg cttcttttcc ttcctgctcg ccttcatggg
    10441 ttcgatgctc ggcatcgtgc tgtccggcaa cctgatccag ctcgtggtgt tctgggaact
    10501 gacaagtttc agctccttca tgctgatcgc gtactggtac caccgcgcgg aggcgcgcca
    10561 gggcgcgcgc atggcgctca ccgtgacggc cactggcgga ttctgcctgc ttgccggggt
    10621 catcatgctc ggtaccgtcg cgggcagctt cgacctcgac agggtgctcg cggccggcga
    10681 cacggtccgt gcgcatccct ggtatccggt catgctcggg ctgatcctgc tcggggcgct
    10741 gacgaagagc gcgcagttcc ccttccattt ctggctgccg cacgcgatgg cggcgccgac
    10801 gcccgtgtcc gcatacctgc actccgcgac cctggtcaag gccggcgtct tcctgctggc
    10861 gcgcctctgg cccgtgctcg cgggcaccga tctgtggttc gcgctcgtct gcggcgcggg
    10921 gctcgcgtcg ctgctgctcg gcgcggccgc ggcgctcttt cagcgtgaca tgaagggcgt
    10981 gctcgcgtac tccaccgtca gccacctcgg gctcatcacg ttgctcctcg gcatgaacag
    11041 tccgctcgca ctcgtcgccg cggtcttcca catcatgaac cacgcgacct tcaaggcgac
    11101 gctcttcatg gcggccggca tcgtcgatca cgaggccggc acacgcgacc tgcgacgtct
    11161 gaatgggttg gcgcgcgcga tgccgatcac cgccacgatc gccaccgtcg ccgcggccgc
    11221 catggccggc gtgccgctgc tcaacggttt tctctcgaag gaaatgttct tcgccgaggc
    11281 gatcttcgcc ggcgaggagc cgctgctgcg cgtcggcctg ccggcacttg caacactcgc
    11341 gggcatcttc agcgtcgcgt attcgctgcg cctcgtgcac caggtgttct tcggcgcgcc
    11401 cgcgcgcgag ctgccgcgca cgccgcacga gccgacccgc tggatgctgt tgccgggggc
    11461 gctgctggtg ctcacctgcc tcctggtcgg catcctgccg gcgcgcacga tcgggccggc
    11521 gctcgcactc gccgtgcaat cgatcctcgg ggccgccacg cctgcctaca gcctcgtcgt
    11581 ctggcacggt ttcactgcgc cattcctgat gagcgtgatc gcgctcgtgg gtggggtcgg
    11641 cctctacctg ctgcgatact tctatggcca gcgcgcgacg ggccgcgcgc cgctgcgcgc
    11701 gccgttcgat ggccggcgcg tgttcgacat cctgattgtc gtgctgcacc gggccgccga
    11761 gcgcatggtg cgcttcgcgt cttcgcgacg ccagcagccc caactcctcc tgatcgtctg
    11821 cctggcgctc gccgtggcag ccatcccgct cgccgcgagc ggtttgtcag ggggtggagc
    11881 gccgctgacc ccgatcgatc ccgcctacgt cctgccctgg ttcgtcggct gcctctgcgc
    11941 gatcggggcg gcggtgcagg cgaagttcca tcggctcgca gcactgatca tgctcggcgg
    12001 agcggggctt ttcaccagcc tgaccttcgc ctggttctcc gcgcccgatc tcgcgctgac
    12061 gcagatcgcg gtcgaggtcg tcacgctcgt actgctgctg ctcgggttgc gctggctgcc
    12121 gaggcgcctc gagctcgacg atccggaccg gcgcacgcgg cgcgcacgcg cgcgccgcgt
    12181 gcgcgacttc ctgcttgccc tggtcgtcgg cggcagcctc gctgcgctgt cctttgcgat
    12241 gctcacccgc gcgccggtga acggcatcgc gcatttcttc atcgggcgcg cgctccccga
    12301 aggaggcggg cgcaatgtcg tcaacgtgat cctcgtcgat ttccgtggct tcgatactct
    12361 cggcgagatc acggtcgtcg cgatcgtcgc gctgtcggtg tacgcgctac tgcggcgctt
    12421 ccggcctgca ctggagagca tcgaggtacc gcgggcgcaa cgtgaggcgc tcgcgcacgg
    12481 gaaacggcct gtctttgatc cggacgcgaa gctgccggcg ggcgatcttg ccgtcccggc
    12541 ggtgctggtg cgcctcctgc tgccgatggc cggcctcgtt gccgtctacc tgctgctgcg
    12601 cggacacgat gcgccgggcg gcggattcgt cggcgggctc gtgatggcca cggcctccat
    12661 cacgcaatac atggtcggcg gcgcccagtg ggtcgagtgg cgcatgcgga ttcatccgca
    12721 gtactgggtg gcgctcggcc tccttgccgc gggcggcgcc ggcgtgctcg cctggtcgat
    12781 cgggcagcca ttcctgacga gtttcggtgc ggagctggcg ctacccgtcg tcggtgacgt
    12841 gcatgtcgcg agcgtgctgc tcttcgatct cggcgtgtat ctcgtggtcg tcggcgcgac
    12901 ctcattgatg ctcgtcgcgc tcgcgcacca gtcgttccgc agccagcggg gggcgtgacg
    12961 atggagatcc tctttgcgat cgcgatcggc gtgctctcgg gctcgggcat ctggctgctg
    13021 ctgcgcccgc gcaccttcca ggtgatcgtc ggtctcgcgc tgctctccca ggcggtcaat
    13081 ctcttcattt tcggcatggg ccggctcgcg accggcaagg ccccgatcct gggcgcgcat
    13141 cccgtcgatc cggcgagcct cgcagatccc gtgccacagg cgctcgtgct caccgcaatc
    13201 gtgatcggtt tcgcgacgac cgcgttgctg ctcgtcgtgc tgcttgcggc tcgcggcctg
    13261 accggcacag accacgtcga cgggcgggag tcttgatgag ctcctggttc cggcaccttc
    13321 cggtcgtacc gatagtcctg ccgctgcttg ccgcggcgct gatcctgctg ctcgccgagc
    13381 gcgcgcgcgc gctgcggatc gcgatcgcat gcgtctcgat caccgtgcaa ctcgtcgcag
    13441 cgatcatgct gctggcgctc acgaccgagg cgttcccgga catctggccg cagggcgtcg
    13501 gggcctacgc gatcggctcg tggccggcgc cgttcggcat cgtgctcgtg atcgatcgcc
    13561 tgacggggtt gatgctgacg ctcacggcgg tcgtggggtt tgcggtgttc atcaattcac
    13621 tcgcgtactg ggaccgggtc gggccgcact ttcacgcgct gctgcagctc ctcctgatgg
    13681 gggtgaacgg tgccttcctg acgggcgacc tgttcaacct cttcgtgttc ttcgaagtgc
    13741 tgctcgccgc atcctatggt ctcctgctgc acggctcgaa ctcgcagcgt gtgaaaatgg
    13801 gcctgcacta catcgtggtg aacctggtcg ggtcgttgct cttcctgatc ggtgtttcgc
    13861 tgatctacgg cgtgaccggt actctcaaca tggcggacct gcccgcgagg gctgcggcgt
    13921 tgtcggacgc agaccgcgcg cttttcgatg ccgccgcggc cgttctcggc atcgcatttc
    13981 tcaccaaggc gggctgctgg ccgctcaact tctggttacc cggcacctat gctgccgcaa
    14041 gcccgccggt cgccgcggcc tttgcgatcc tgaccaaggt tggtgtgtac gcgctgctgc
    14101 ggctcggctc gctgatcggc agcgccaatg cgctcgcgca tatcgaaggc tcggcgctgt
    14161 tgcggccgga tctctttcgc ggcgggatgt tctacatcgg aatcatgacc atgggcttcg
    14221 gcattttcgg cacgctcgcc gcccagcagc tcgcacgcgt cgtcggcttt tccgtgatcg
    14281 tctcttccgg cctgctgctc acgatcatgg ggctcgacca cacaccactg ctcgcgccgg
    14341 ggctgtttta tctcgtcggc tcggtgctcg cgaccagcgc cttcttcatg ctcacgggca
    14401 tgaccgggcg cacgtggcgc actccgacgg tgccggcgac ggtcccggcc acaccggtgt
    14461 ccgtgcccac gagtggctac atacccttcg gcgtgcgtct cgcagccgcg ccgcatggcg
    14521 aggacgacga agtgggcgtt gcgatccccg cggcgatggc gttcatgggt ctcatgttcg
    14581 cgttctgcgt gctgctcgtc acggggtctc cgccactctc ggggtttgtc gcgaagttcg
    14641 cgatccttgc cgctgcgata caggcggcgc aaggcgcgcc gtgggctgcg gccgcgtgga
    14701 tgctggtggg cttgacgatc gcagcgggcc tcgcaagcct cattgcgctg actcgcatcg
    14761 gcatgcgcct cttctggtcg gtgaccgggc gcagtacgcc gcgtcttcgc gcgctcgaag
    14821 cggggcccgt ggcgatgctg gtgttcctct gcatcgcgct gacagtggcc gcggggcccg
    14881 cgatgggctt tctggaggac acggcagcgt cgctgcgcgc gccccggatc tacatcgaca
    14941 cggtgctcgc caccggggag gggcgatgaa aggcggggtg cccgtgctcg gcggtgcgct
    15001 cgttgcgatg tggcttgcgc tcaacgacac ggtcgcgccc gggcaagtgc tgcttggcgc
    15061 gctgatcgcc gtggcgctca tcctgtgggc ggcgaagctg cgtcccttgc ggccacgcct
    15121 gtacaggccg tggctcgcca tcagcctgct ggccgtggtt ttcgtggata tcgtgcgctc
    15181 gaacattgcg gtcggtcgca tcatcctggg gctcgttccc gttcgcgcga tccgctccgg
    15241 gctgctcgag attccgctcg atctgcacga tccacacgga cttgcggtac tcgcaacgat
    15301 catcacctcg acgccgggca ccgtgtgggt cgaacatgtg gccgagcgcc gcacgctcac
    15361 gctgcacatc ctcgatctca aggacgaggc ggggtggatc cgcgacatca aggaccgcta
    15421 cgaacggccg ctgatcagga tcttccagcc gtgagcactc tgattcacca ggccgcca
//
LOCUS       DAORUQ010000065        15291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3654_length_15291_cov_6.311630, whole genome shotgun sequence.
ACCESSION   DAORUQ010000065 DAORUQ010000000
VERSION     DAORUQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15291
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_15291_cov_6.311630"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3504)
                     /locus_tag="PLW13_07590"
     CDS             complement(<1..3504)
                     /locus_tag="PLW13_07590"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQY70273.1"
                     /translation="MNVERAQTTMKDITLDDKYTATTGRVYLSGAQALVRLPLMQRQR
                     DQAIGLNTAGFISGYRGSPLGVYDLALWQAERFLREHHVHFQPGVNEDLAATAVWGSQ
                     QVKDIGESPWDGVFALWYGKGPGVDRSGDPLKHGNYAGSSRHGGVLALCGDDHGARSS
                     TIAHQSDHALIHFGMPILFPATIQEYLDLGLHGFALSRYAGCWVGFKCVTDVVEGSAS
                     VAVDPARVRIVEPRDFTAPAGGLNLKLGMFPLAAEAQMLARLEAAKAYVRANGLDRVI
                     WPKARARLGIVSTGKAWLDVMDALPQLGIDEARAAELGVAVYKVAMVWPLEPAGIADF
                     AAGCEQLLVIEEKRPVIEEQIAGLLFNQPPGWRPRLTGKRDADGAPLVPSGGELNPLL
                     LARLIGAQLLRREPDAALAARLAALEPPAAVGGFAGGQGALMRLPSFCAGCPHNTSTR
                     VPEGSMAFGGIGCHGLATWLPERNTPTLYHMGGEGAAWIGQAPFTTREHVFQNLGDGT
                     YFHSGLLAIRANVAAGTNITYKILVNGAISMTGGQPIEGESFDGGITAPHVAQQLHAE
                     GVKRIALVTEDLARHRERAKFPAITTFHHRDELDAVQRELREHPGVSAIVYEQACATE
                     RKRLRKRGRYPDVDKRYLISEEVCEGCGDCGLQSNCIALEPNETRFGRKRTINQSVCN
                     KDFSCVKGMCPSFVTVYGGRLRATTGAGEQGIDESLFAALPEPVIRAGAGACNILVAG
                     IGGSGIITLGALLGIAAHMEDKPVSVLDITGLAQRNGPVTSHIRFVADGDIGVATRIP
                     EGSADLVLAADLVVAAGAEVLARMSGTRSAVVYNAHVAPTNAFARNPDLDFDSAGMEA
                     ALAARTRADGVLRLDATRIATALLGNAIGANLFLLGAAWQRGLVPLARASLERAIALN
                     ATAVEMNLRAFSLGRLAVSNPAALEALLVEPQPVRLLASGRETLDSLVADRLAWLTAY
                     QDAAWGARYEQLVRRVAATEREKTGMSGALAEAVARNLAKLMAYKDEYEVGRLLSGEV
                     LRHQLETQFEGDYRIAFNLAPPLLAARDPATGRYRKREFGAWMLPVFGVLARLKFLRG
                     TPLDVFGWSAHRRRERALIGEYEQLVETLLAGLHRGNHALAVKLAALPERVRGFDVVK
                     EAAMEQAATARATLLAEFAAAP"
     gene            complement(3494..4285)
                     /locus_tag="PLW13_07595"
     CDS             complement(3494..4285)
                     /locus_tag="PLW13_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY70274.1"
                     /translation="MEFSTLKYELTDGVALITLDRAQAHNAINSVMSRELPAVWGHFA
                     ADDAALVAIVTGAGERALCVGADLADLPATDGEGRNGTLASIRWTSLQNAVWKPVICA
                     VNGMAVGGGLHFVADSDIVIAAEHASFFDTHVKVGLVAGMEPVTLARKMPLEAVLRMT
                     LVGGNERMDAARAQALGLVGEVVPADRLLARAFEVAAMIKGNSPAAMARSKRAIWEGA
                     ERGLGAALEHAWELIMQQNAHPDIEEGARAFLEKRAPRWRPFSEC"
     gene            complement(4289..5434)
                     /locus_tag="PLW13_07600"
     CDS             complement(4289..5434)
                     /locus_tag="PLW13_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70275.1"
                     /translation="MSIAGKAAIIGAGCSAFAEHWTKSAEDLVVDAVLEAYADAGIVE
                     PRRQIDAVFAGSLYAPMGPVHVTEALKLYKPTTMVYNYCATGTEALRAAVMAVASGAH
                     ETVLAVGFDKPKDRGVSGPSVNIRGVRELPETPAGWFALCAAPYFARFGAGREDLARI
                     AVKNHHNGTLAPKSFLKKEITVEEALGARMIAWPFGLYDCAAQTDGAAAVIVTRRDLA
                     RNYHDRAVIVRAVASASAPHPHTDPAHDFLRWKPTVAAAEAAYAIAGISDPMRAIDVA
                     QLHDCFTLTELLSYEDLGFIAKGAAREHIGAGTFALGGELPVNTDGGLKAFGHPTAAT
                     GLRMVYENVLQLQGRAGARQVAGATTALSHNIGGYPTGCAVSILSRE"
     gene            complement(5431..6828)
                     /locus_tag="PLW13_07605"
     CDS             complement(5431..6828)
                     /locus_tag="PLW13_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY70276.1"
                     /translation="MTGIVACGAWIPARRLPLPLLAGKAARDGAPERALAWADEDALT
                     MAVEAARDCLRGRDRGDIDLLLFASTTGPYAEKQGAAIIAAALGLDPGVRTVDVGSSL
                     RGGTQALLLALDAVRGGSVRAALVVATDCREGAPGSALEANGGDAAAALLVGNGETLV
                     ELVDSASESAEIVDVWRRHGDRFVHAWEDRFVIAHGYLEPARAAVRRLNERGRFDTGV
                     CDHLVLSAPDRKSHESLARVLGGLATVADPLFGSVGACGSAHALLQLVALAETAEPGA
                     SALLVNHGDGADALGLRFRRKAEARMTARLARRLPLTSPDQYRRARELLVTEYAGNDF
                     QGISATVHRRERAEDLALAGQRCACGHAQFPRERICGACGVADRFTDEVFAERRGRLL
                     TYTLDAFFPTPEPPTAVGVVQVEDGPRIYLQLTDVPASDVALGMALEFTFRRIHTVGG
                     RPNYFWKCRPRGEAA"
     gene            complement(6833..7726)
                     /locus_tag="PLW13_07610"
     CDS             complement(6833..7726)
                     /locus_tag="PLW13_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70277.1"
                     /translation="MRLLEGKRAVVTGAGGGIGRAHARLLAAHGAAVVVNDVDAARAA
                     AVVAEIGAAGGSAVANGDSIATRAGAEALIAACVDAFGGIELLVNNAGNLRDRSFLKM
                     SDEEFDAVWLVHVKGSFWCAQAAARRMQAQGRGGVIINTSSGSHFGNFGQTNYAAAKG
                     AIASMTYTWAIELARFGIRVNAICPVAATQMTHGLMSAPGGQAPEQLWLPERNAPLVV
                     YLASEEAANVSGQVFGVGGERLAHMVQPHYGKTLTHPGGWTVEAIRERFQREMPPEFG
                     AFGILGKPYPFHDGVHAPDEE"
     gene            complement(7723..8838)
                     /locus_tag="PLW13_07615"
     CDS             complement(7723..8838)
                     /locus_tag="PLW13_07615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70278.1"
                     /translation="MDLDFSDEQTLLRDTVRSLCADLLDSQQLRVLECDERGYSRRVW
                     RALGETGVTGVLVPESHGGVGLGLIDCAIVHEELGRCLAPVPHIPSCVLAARLIALAG
                     SDAQQAEWLPAIAAGERIVAPAWREPGNSADIERVALAARQAQGRIVLEGVKTLVPFA
                     NSADALLVIARLGGELVALLVPSDSAGIRLVDLPNHAGQKLMRVEFAEVAVDAQNLFD
                     RAPLAEAWQRAMHETLVALAAESIGGAARMLEMAAAYAKERVQFGQPIGAFQAIAHYL
                     ADAATEIAGVRWLVYQAAWAADTGRPFEQLALMAKLQAGAVYRRATHKGVQIHGGLGF
                     SLDADPQLFYRRAKHQQLMYWDPAYLERRIAAGVFGA"
     gene            complement(8856..9542)
                     /locus_tag="PLW13_07620"
     CDS             complement(8856..9542)
                     /locus_tag="PLW13_07620"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70279.1"
                     /translation="MDSENQSVRGRGRPRSQASEKAILDATMDLLSELGFRAVTVDAI
                     AERAGASKATIYRRWPAKENLVIAAFASTPLLAVRGRGDTVSQLVDVQAQFARFMQET
                     TLGGVLAALVAERAHNPALGVAMDPLVDQRRQPMAEILRRAIERGELAEDLDIEFAID
                     MISAPTLQRIMVMGLPTDRGYIRRVVETAIRGLGGSARGETVLKARAKAPAKARAKAP
                     GKAPASTKQK"
     gene            complement(9555..10742)
                     /locus_tag="PLW13_07625"
     CDS             complement(9555..10742)
                     /locus_tag="PLW13_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70280.1"
                     /translation="MDFEFSPEELDFVADVRRFIAAQKALPDAAEVMAPQREADSMLA
                     DSPARRAFNRRLAEAGYLGMSWGREYGGQEKSGVHDYLLNEELASVGAPLIGKGVGCI
                     GKTLIAHGSEKLKREFLPKILRAEIEFALGYSEPAAGSDLASLQLRAVRDGDDWVLNG
                     QKMWTTSAHFADWYWVAARTDPDAPKHKGISVFLLPMDHPGLTVTEIRTMGDHRTNQV
                     FFDDVRVPGDYLVGNANEGWTYVCEALDYERFTMFTVGPIRVKFDALIELLRITRRDG
                     AALADDPLLRRSVARLAAELETATMLQRRVIAAAMKGAVPTVEAAMYKLYSTELGRRM
                     ADLALDALGPEGLLRHDAPDAPAAGKWEHSYRATVVDTIGGGSSEVQKNIIARRGLGL
                     PLR"
     gene            complement(10756..11163)
                     /locus_tag="PLW13_07630"
     CDS             complement(10756..11163)
                     /locus_tag="PLW13_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY70281.1"
                     /translation="MAMQLQKAVLDAGIVTRDLERAARFYGEVLGLPAAGEIAIPGIG
                     LVRRYAVGESTLRIFMPETLPVEQEHGDGFASRTGIRYLTLAIANLEQTVAAVAAAGF
                     RVPMAPKQLRPGVRVAQIQDGDGNTIELMQHDA"
     gene            complement(11201..12172)
                     /locus_tag="PLW13_07635"
     CDS             complement(11201..12172)
                     /locus_tag="PLW13_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY70282.1"
                     /translation="MKICALGYIGIGVPDPRACLDYATSVLGLMPARACPGEDWGMPA
                     VPGSGPASAGSGIAADGSVYLKLDEWQWRIGVHPRAGRAGLMYLGLEVAGALELEQAL
                     AELRAAGCDARAGTEAEARARAVTGMGHTRDPMGNAIELFYGPVKDRKFVSPAGMEFV
                     AGTLGVGHINLLAAPLVAARDFYTRVLGLRLTDFIRFGPGDSANFFHCNARHHSIGLL
                     KVGDLAGVHHLMLEARDIDMVGRCLQRVEEAGIPITSTLGRHANDRMLSFYMASPFGF
                     EVEIGCEGVRVDEHWTPSEFVEGDIWGHRGLDPETIQRNLAAMGGQG"
     gene            complement(12169..13365)
                     /locus_tag="PLW13_07640"
     CDS             complement(12169..13365)
                     /locus_tag="PLW13_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY70283.1"
                     /translation="MPLDYDPFSDAAMRDPFPLYAAMRAEGRPHFIPQYNAWALTRFA
                     DVWEASMHHEADVTWTAGALPTQLLLGEPVPHAFTTMDAPDHRKWRGVVRKEYTPEGV
                     RAHESRIRTLVREVLEPLVARGRFDLYSDYANRVFCLNSGYNLGLPREDAITWRALID
                     DMIHRDRGQRGSSERNQRAAAQLGGYLAEYVQMLRRHPDRASGHTAAYMTTEIDGRRL
                     DDQDMVNLLMNFLIVGSETTPMVCAGALYYLERHPAQKAAVLADHGLVQQLFLETARF
                     DQPTNMLCRRAVRDFTLGGADIKAGQDLLYIYASANRDEAEFERADEFDIFRRYERDL
                     TYGAGGHKCLGMHLATMAGVIVIEEFLKAAGDYAVLVDECERAYGEHLSGFLHVPVQT
                     TTTREA"
     gene            complement(13440..14510)
                     /locus_tag="PLW13_07645"
     CDS             complement(13440..14510)
                     /locus_tag="PLW13_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY70284.1"
                     /translation="MAGQNPRHPPQGVPAQFALTLLQEVAAIGGDPDGLLARLGLPFT
                     QADLVAGRMQVISDHDFVQLYGECITVLSAHANRERDLPPMSKDEVDMLCYCVITCAT
                     LEEVIERARRFCAMLNHRAADLSLDVNGDEAVFHMATQRLRHSVSGLLTDLNGLSFYH
                     RLFAWLIGERIPVSRYDLYAEPLVDRATLERFFQQPIRLGCPDNSFRFPARLLAKPVV
                     RSYQRLVERLSVFPFDHLREPRDAGQFAEAVEHIITTRLARQQSLPTLEQFASVFNLS
                     RATFQRRLREEDVTLDAIKQRVRLKLAQELLHPAARIKVSEVALRLGFSDVRSFRRAF
                     IEWTGETPDEWRRRNPATPRSD"
     gene            14720..>15291
                     /locus_tag="PLW13_07650"
     CDS             14720..>15291
                     /locus_tag="PLW13_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQY70285.1"
                     /translation="MRIPPREVRYGGAMIDQKEIDAVVNVMQTGMSVGTQVQTFESRC
                     AKLLGKEYGVMVNSGSSALLVAVRLLDLPPGSEIITPTLTFGTDISCIVLNGHVPVLV
                     DVEPDTYQVDIDKIERNITPLTKALLIPSLVGGMPDWDRLRALADKHGLKIIEDSCDT
                     LGGTYRGTPAGNRSDISVTSFSIFHIITCLG"
BASE COUNT         2285 a         5531 c         5160 g         2315 t
ORIGIN      
        1 cggtgccgcg gcgaactcgg cgagcagcgt cgcacgcgcc gtagccgcct gctccatcgc
       61 tgcctccttc accacgtcga agccgcgcac gcgctcgggc agcgccgcga gcttcaccgc
      121 gagtgcgtgg ttgccgcggt gcaacccggc gagcagcgtc tcgacgagct gttcgtactc
      181 gccgatcagc gcccgctcgc gccggcgatg cgccgaccag ccgaacacat cgagcggcgt
      241 gccgcgcagg aacttcagcc tggcgagcac gccgaatacc ggcagcatcc aggcgccgaa
      301 ctcgcgcttg cggtagcgcc ccgtggccgg gtcgcgcgcg gcgagcagcg gcggcgccag
      361 gttgaaggcg atgcggtaat cgccctcgaa ctgcgtctcg agctggtgcc tgagcacctc
      421 gccgctcagc aggcgcccca cctcgtattc gtccttgtac gccatgagtt tcgccaggtt
      481 gcgcgccacg gcctcggcca gtgcgccgct catgccggtc ttctcgcgtt ccgtcgcggc
      541 gacgcggcgc accagctgtt cgtagcgcgc gccccacgcg gcatcctggt aggcggtgag
      601 ccaggcgagg cgatcggcga ccaggctgtc cagcgtctcg cgacccgagg cgagcagccg
      661 caccggctgc ggctcgacca gcagggcctc gagcgccgcg gggttgctca ccgcgagccg
      721 cccgaggctg aaggcgcgca ggttcatctc caccgcggtg gcgttcagcg cgatcgcgcg
      781 ctccaggctc gcgcgagcga gcggcaccag gccgcgctgc caggccgcgc cgagcaggaa
      841 caggttcgcg ccgatggcgt tgccgagcag cgcggtggcg atgcgcgtgg catcgaggcg
      901 caacacgccg tcggcgcgcg tgcgcgcggc cagcgcggcc tccatgcccg cgctgtcgaa
      961 atcgagatcg ggattgcgcg cgaaggcatt ggtaggcgcc acgtgcgcgt tgtagaccac
     1021 cgccgagcgc gtgccggaca tgcgcgccag cacctccgca cccgccgcca ccaccaggtc
     1081 cgcggccagc accaggtccg ccgagccctc gggaatgcgc gtcgccacgc caatgtcgcc
     1141 gtccgcgacg aagcggatgt ggctggtcac gggaccgttg cgctgagcca gcccggtgat
     1201 gtcgagcacg ctgaccggct tgtcctccat gtgcgcggcg atcccgagca gcgcgcccag
     1261 cgtgatgatg ccgctgccgc cgatgccggc gacgaggatg ttgcaggccc cggccccggc
     1321 acggatgacc ggttcgggca gcgccgcgaa cagcgactcg tcgatgccct gctccccggc
     1381 gccggtcgtc gcgcgcaggc gcccgccgta gaccgtgacg aagctcgggc acatgccctt
     1441 cacgcaggag aagtccttgt tgcacaccga ctggttgatc gtgcgcttgc gcccgaagcg
     1501 ggtctcgttg ggttccagcg cgatgcagtt cgactgcagg ccgcagtcgc cgcagccctc
     1561 gcagacttcc tcgctgatca ggtagcgctt gtcgacgtcg ggatacctgc cgcgcttgcg
     1621 caggcgcttg cgctcggtgg cgcaggcctg ttcgtagacg atggccgaga cgccggggtg
     1681 ctcgcgcagc tcgcgctgca ccgcatcgag ctcgtcgcgg tggtggaagg tggtgatcgc
     1741 gggaaacttc gcgcgctcgc ggtggcgcgc caggtcctcg gtgaccagcg cgatgcgctt
     1801 cacgccctcg gcatgcagtt gttgcgccac gtgcggcgcg gtgatgccgc cgtcgaagct
     1861 ctcgccctcg atgggctggc cgccggtcat cgagatcgcg ccgttcacga ggatcttgta
     1921 ggtgatgttc gtgccggcgg cgacattggc gcggatcgcg agcagcccgg aatggaaata
     1981 ggtgccgtcg ccgaggttct ggaacacgtg ctcgcgcgtc gtgaagggcg cctgcccgat
     2041 ccacgccgcg ccctcgccgc ccatgtggta gagcgtcggg gtgttgcgct cgggcagcca
     2101 ggtcgccagc ccgtggcagc cgatgccgcc gaacgccatc gagccctcgg gcacgcgcgt
     2161 cgaggtgttg tgcgggcagc ccgcgcagaa gctcggcagg cgcatcagcg cgccctgtcc
     2221 gccggcgaat cccccgaccg ccgccggcgg ctcgagggcc gcgagccgcg cagccagcgc
     2281 ggcatcgggc tcgcggcgca gcagctgcgc gccgatcagg cgcgcgagca gcagcggatt
     2341 cagctccccg cccgagggca ccagcggcgc gccgtcggcg tcgcgcttgc cggtgagccg
     2401 cggccgccag cccgggggct ggttgaacag caggcccgcg atctgctcct cgatgacggg
     2461 gcgcttctcc tcgatcacca gcagctgctc gcagcccgcg gcgaagtccg cgatgcccgc
     2521 gggctcgagc ggccacacca tcgcgacctt gtagaccgcc acccccagct cggcggcgcg
     2581 cgcctcgtcg atgccgagct gcggcagcgc gtccatcacg tcgagccagg ccttgccggt
     2641 gctgacgatg cccaggcgcg cgcgcgcctt gggccagatc acgcggtcga ggccgttggc
     2701 gcgcacgtag gccttcgcgg cctcgagacg cgccagcatc tgcgcctcgg ccgcgagcgg
     2761 gaacatgccc agcttgagat tcaggccgcc cgccggcgcc gtgaaatccc ggggctcgac
     2821 gatgcgcacg cgtgcgggat ccacggccac cgaggccgag ccctcgacca cgtcggtcac
     2881 gcacttgaag cccacccagc agccggcgta acgcgagagc gcgaagccgt ggagcccgag
     2941 gtcgaggtat tcctggatcg tggccgggaa caggatcggc atgccgaaat ggatcagcgc
     3001 gtggtcgctc tggtgcgcga tggtcgagga gcgcgcgccg tggtcgtcgc cgcacagcgc
     3061 cagcaccccg ccgtggcgcg agctgcccgc gtagttgccg tgcttcagcg ggtcgcccga
     3121 gcggtccacc ccggggccct tgccgtacca cagcgcgaac acgccgtccc agggggattc
     3181 gccgatgtcc ttcacctgct gcgagcccca caccgcggta gccgcgaggt cctcgttgac
     3241 accgggctgg aagtgcacgt gatgctcgcg caggaagcgc tcggcctgcc acagcgcgag
     3301 gtcgtagacg ccgagcggcg agccgcgata accggaaatg aagcccgcgg tgttgagccc
     3361 gatcgcctgg tcgcgctggc gctgcatcaa tggcagacgc accagcgcct gcgcgccgct
     3421 caggtagacc cgcccggtcg tggcggtgta cttgtcatcc agcgtgatgt ctttcatggt
     3481 cgtctgcgct cgctcaacat tcactgaagg gacgccagcg cggcgcgcgt ttctcgagga
     3541 aggcacgcgc gccctcctcg atgtcgggat gcgcgttctg ctgcatgatc agctcccagg
     3601 catgttccag cgcggcgccg agaccgcgct cggcgccctc ccagatcgcg cgcttgctgc
     3661 gcgccatcgc ggccggcgag ttgcccttga tcatcgcggc gacctcgaag gcgcgcgcca
     3721 gcagccgatc cgcgggcacc acctcgccca ccagtcccag tgcctgcgcg cgcgcggcat
     3781 ccatgcgctc gtttccgccg accagcgtca tgcgcagcac cgcctccagc ggcatcttgc
     3841 gcgccagtgt caccggctcc attcccgcca ccagccccac cttcacgtgc gtgtcgaaga
     3901 agctcgcgtg ctcggcggcg atcacgatgt cgctgtcggc cacgaagtgc agtccgccgc
     3961 ccaccgccat gccgttcacc gcgcagatca ccggcttcca taccgcgttc tgcagcgagg
     4021 tccagcggat gctcgccagc gtgccgttgc ggccctcgcc gtccgtggcc ggcaggtccg
     4081 cgaggtccgc gcccacgcag agcgcgcgct cgccggcgcc ggtgacgatg gcaaccagcg
     4141 ccgcgtcgtc cgcggcgaag tgcccccaca cggccggcag ctcgcggctc atcaccgagt
     4201 tgatcgcgtt gtgcgcctgc gcacggtcca gcgtgatcag cgccacgccg tcggtgagct
     4261 cgtatttcag tgtgctgaat tccatcgcct actcccggct caggatgctg acggcgcagc
     4321 ccgtggggta gccgccgatg ttgtggctga gcgcggtcgt tgcccccgcc acctgccgcg
     4381 caccggcgcg tccctgcagc tgcagcacgt tctcgtagac catgcgcaga cccgtggccg
     4441 ccgtgggatg cccgaaggcc ttgagaccgc cgtcggtgtt caccggcagc tcgccgccga
     4501 gcgcgaaggt gccggcgccg atgtgctcgc gcgccgcgcc cttggcgatg aagcctagat
     4561 cctcgtagct cagcagctcg gtgagcgtga agcagtcgtg cagctgcgcc acgtcgatcg
     4621 cgcgcatcgg atcgctgatg cccgcgatcg cgtaggccgc ttcggccgcc gcgaccgtgg
     4681 gcttccagcg caggaagtcg tgcgcgggat cggtgtgcgg atgcggcgcc gaggcgctcg
     4741 ccaccgcgcg cacgatcacc gcgcggtcgt ggtagttgcg cgccagatcg cgccgcgtga
     4801 cgatcaccgc ggccgcgcca tcggtctgcg cggcgcagtc gtagaggccg aagggccatg
     4861 cgatcatgcg cgcgcccagc gcttcctcga ccgttatctc tttcttcagg aagctcttcg
     4921 gcgccagcgt gccgttgtgg tggttcttca ccgcgatgcg cgcgagatcc tcgcgcccgg
     4981 cgccgaaacg cgcgaaatac ggcgccgcgc acagcgcgaa ccagccggcc ggcgtctcgg
     5041 gcagctcgcg cacgccgcgg atgttcacgc tgggccccga gacgccgcgg tccttgggct
     5101 tgtcgaagcc cacggcgagc acggtctcgt gcgcgcccga ggccaccgcc atcaccgcgg
     5161 cgcgcagcgc ctcggtgccg gtggcgcagt agttgtagac catcgtggtg ggcttgtaga
     5221 gcttcagcgc ctcggtgacg tgcaccgggc ccatcggcgc gtacagcgag ccggcgaaca
     5281 ccgcgtcgat ctggcgccgc ggctcgacga tgcccgcgtc cgcgtaggcc tccagcaccg
     5341 cgtccaccac caggtcctcg gcgctcttcg tccagtgctc ggcgaaggcg ctgcaaccgg
     5401 cgccgatgat cgctgccttg cccgcgatgc tcatgctgcc tccccgcgtg gccggcattt
     5461 ccagaaataa ttgggccgcc cgcccacggt gtggatgcgc cggaacgtga actcgagcgc
     5521 catgccgagc gccacgtcgg acgccggcac gtcggtgagc tgcaggtaga tgcgcggccc
     5581 gtcctcgacc tgcaccacgc cgaccgccgt gggcggctcc ggcgtgggga agaaggcatc
     5641 gagcgtgtag gtgagcagcc gcccgcggcg ctcggcgaac acctcgtcgg taaatcgatc
     5701 cgccacgccg caggcaccgc agatgcgttc gcgcggaaac tgcgcgtgac cgcaggcgca
     5761 gcgctggccc gcgagagcca ggtcctcggc gcgctcgcga cgatgcaccg tggccgagat
     5821 gccctggaag tcgttgccgg cgtactcggt gaccagcagt tcgcgggcac gacggtactg
     5881 gtccggactg gtgagcggca gccggcgcgc gagccttgcg gtcatgcgcg cctcggcctt
     5941 gcgacggaaa cgcagcccca gcgcatcggc gccgtcgccg tggttgacca gcagcgcact
     6001 ggcgccgggc tcggcagttt cggccagcgc gaccagttgc agcagggcgt gtgcgctgcc
     6061 gcaggcgccg acgctgccga acagcgggtc ggcgacggtt gccaagccgc cgagcacacg
     6121 cgcgagcgat tcgtgcgact tgcgatccgg cgccgagagg accaggtgat cgcagacgcc
     6181 ggtatcgaag cgtccgcgct cgttcaggcg gcgcaccgcg gcgcgagcgg gctcgagata
     6241 gccgtgcgcg atcacgaagc ggtcttccca agcgtgcacg aagcggtcac cgtggcggcg
     6301 ccacacgtcg actatctcgg cgctctcgct cgcgctgtcg acgagctcga ccagcgtctc
     6361 gccgttgccg accagcaaag ccgcagccgc gtcgccgccg ttggcttcga gcgccgagcc
     6421 cggggccccc tcgcggcagt ccgtggccac caccagcgcg gcgcgcaccg aaccgccgcg
     6481 caccgcgtcc agcgccagca acagcgcctg cgtgccgccg cgcagcgagc tgcccacgtc
     6541 cacggtgcgc acgcccgggt cgagccccag cgcggcggcg atgatcgccg cgccctgctt
     6601 ctcggcgtag ggacccgtgg tgctggcgaa cagcagcagg tcgatatcgc cgcgatcgcg
     6661 cccgcgcagg caatcgcgcg cggcttcgac cgccatcgtc aatgcgtcct cgtcggccca
     6721 ggccagcgcg cgctccggag cgccatcgcg cgccgccttg cccgcgagca ggggcaacgg
     6781 caggcgccgc gccggaatcc aggccccgca cgccacgatt cccgtcatcg cgtcattcct
     6841 cgtccggggc atgtaccccg tcatgaaagg gatagggttt gccgaggatg ccgaacgccc
     6901 cgaactcggg cggcatctcg cgctggaagc gctcgcggat ggcctccacg gtccagccgc
     6961 ccggatgcgt cagcgtcttg ccgtagtgcg gctgcaccat gtgcgccagc cgctcgccgc
     7021 ccacgccgaa gacctggccg ctgacgttcg cggcctcctc cgatgcgagg tagaccacca
     7081 gcggcgcgtt gcgctcgggc aaccacagtt gctcgggcgc ctgcccgccg ggggcgctca
     7141 tcagcccgtg cgtcatctgt gtggccgcga ccgggcagat cgcgttgacg cggatgccga
     7201 agcgcgcgag ctcgatcgcc caggtgtagg tcatcgaggc gatcgccccc ttggcggcgg
     7261 cgtagttggt ctggccgaag ttgccgaaat gcgagcccga ggaggtgttg atgatcacgc
     7321 cgccacgacc ctgcgcctgc atccgccgcg ccgcggcctg cgcgcaccag aagctgccct
     7381 tcacgtgcac cagccacacc gcgtcgaatt cctcgtcgct catcttcagg aaggaacggt
     7441 cgcgcaggtt gccggcgttg ttgaccagca gctcgatgcc gccgaaggcg tccacgcagg
     7501 cggcgatcag cgcttcggcg cccgcgcgcg tggcgatgct gtcgccgttg gcaacggcgc
     7561 tgccgcctgc cgcgcctatc tcggcaacga ccgccgcggc gcgcgccgca tccacatcgt
     7621 tgaccacgac cgccgcgccg tgcgccgcca gcagccgcgc gtgcgcgcgc ccgatgccgc
     7681 cgccggcgcc ggtgaccacg gcgcgcttgc cctcgagcag cctcatgcgc cgaacactcc
     7741 cgcggcaatg cgccgctcca ggtacgccgg atcccagtac atcagctgct ggtgcttggc
     7801 ccggcggtaa aagagctgcg gatcggcatc gagactgaag ccgagtccgc cgtggatctg
     7861 cacgcccttg tgcgtcgcgc ggcggtagac cgcgccggcc tgcagtttcg ccatcagcgc
     7921 cagctgctcg aaggggcgcc ccgtgtcggc cgcccacgcg gcctggtaca cgagccagcg
     7981 cacacccgcg atctcggtgg cggcatccgc gaggtaatgc gcgatcgcct ggaaggcgcc
     8041 gatcggctgc ccgaactgca cgcgctcctt ggcgtaggca gcggccatct ccagcatgcg
     8101 cgcggcgccg ccgatcgact ccgccgccag cgcgaccagc gtctcgtgca tggcgcgctg
     8161 ccaggcttcg gcgagcggcg cgcgatcgaa cagattctgc gcatccaccg cgacctcggc
     8221 gaattccacg cgcatcagct tctgcccggc gtgattgggc agatcgacca ggcggatccc
     8281 ggcgctgtcg ctcgggacga gcagcgcgac gagctcgccg ccgagccgcg cgatgacgag
     8341 cagggcgtcg gcgctgttgg cgaagggcac cagggtcttc acgccttcca gcacgatgcg
     8401 cccctgtgcc tgtcgcgcgg cgagcgcaac gcgctcgata tccgcggagt tgcccggctc
     8461 ccgccacgcg ggcgccacga tgcgctcgcc ggcggcgatc gccggcagcc actcggcctg
     8521 ctgggcatcg ctgcccgcca gcgcgatcag gcgcgccgcc agcacgcagc tcgggatgtg
     8581 cggcaccggc gccaggcaac ggcccagctc ctcgtgcacg atcgcgcagt cgatcaggcc
     8641 gagacccacg ccgccatgcg actcgggcac cagcacgccg gtcacgccgg tctcgccgag
     8701 cgcacgccac acgcgcctgc tgtagccgcg ctcgtcgcac tccaggacac gtaactgctg
     8761 gctgtcgagc aggtcggcgc agagactgcg cacggtatcg cgcagcagcg tctgctcgtc
     8821 ggagaaatcc aggtccattc cttcctctcc cttgttcact tctgcttcgt gctcgccggc
     8881 gccttgcccg gcgccttcgc ccttgccttc gctggcgctt tcgcccgagc cttcagtacc
     8941 gtttctcccc gggccgatcc gcccaacccc cggatcgccg tctcgaccac ccggcgaatg
     9001 tagccgcggt cggtcggcag ccccatgacc atgatccgct gcagcgtggg tgcggaaatc
     9061 atgtctatcg cgaactcgat gtcgaggtct tcggccagtt ccccgcgctc gatcgcgcgc
     9121 cgcaggattt ctgccatcgg ctggcgccgc tggtccacca gcggatccat cgccacgccc
     9181 agcgccggat tgtgcgcacg ctcggccacg agagcagcga gtacgcctcc cagcgtggtc
     9241 tcctgcatga agcgcgcaaa ctgcgcctgc acgtcgacca gctgcgagac cgtgtcaccg
     9301 cgtccgcgca ccgccagcag cggcgtgctc gcgaaggccg cgatcaccag gttctccttc
     9361 gccggccagc gccggtaaat ggtcgccttg ctggcaccgg cgcgctcggc gatcgcatcg
     9421 acggtcacgg cgcgaaagcc cagctccgac agcaaatcca tcgtcgcgtc gagaatggcc
     9481 ttctccgatg cctggctgcg cggccgcccg cgcccccgga cggactgatt ctccgaatcc
     9541 atggcttctc ctgcctagcg gagcggcaga cccaggccac gccgcgcgat gatgtttttc
     9601 tgcacctcgg aggagccgcc gccgatggtg tccacgaccg tggcccggta cgaatgctcc
     9661 catttgcccg cggcgggcgc atcgggcgca tcgtggcgca gcaggccctc ggggccgagc
     9721 gcatcgaggg ctaaatccgc catgcgccgc ccgagctcgg tgctgtagag cttgtacatc
     9781 gccgcttcca ccgtgggcac ggcgcccttc atcgcggcgg cgatcacgcg ccgctgcaac
     9841 atcgtcgcgg tctccagctc ggcggcgagc cgcgcgacgc tgcggcgcag cagcgggtcg
     9901 tcggcgagcg cagcgccgtc gcggcgcgtg atgcgcagca gctcgatgag cgcatcgaac
     9961 ttcacgcgga tcgggcccac ggtgaacatc gtgaagcgtt cgtagtcgag cgcttcgcag
    10021 acataggtcc aaccctcgtt ggcgttgccg accaggtagt cacccggcac gcgcacgtcg
    10081 tcgaagaaca cctggttggt gcggtggtcg cccatggtcc ggatctccgt gaccgtgagg
    10141 cccgggtgat ccatcggcag caggaacacg ctgatgccct tgtgcttggg agcgtccgga
    10201 tccgtgcgcg ccgcgaccca gtaccagtcg gcgaagtgcg ccgaggtggt ccacatcttc
    10261 tggccgttca gcacccagtc atcgccgtcg cgcacggcgc gcaattgcag cgatgcgaga
    10321 tcggaacccg ccgcgggttc ggaataaccg agtgcaaact cgatctcggc gcgcaggatc
    10381 ttcggcagga actcacgctt gagcttctcg gagccgtgcg cgatcagcgt cttgccgatg
    10441 cagcccacgc ccttgccgat cagcggcgcg cccacgctcg cgagctcctc gttcagcagg
    10501 tagtcgtgga cgccggactt ctcctgcccg ccgtattcgc gcccccagct catgccgagg
    10561 tagccggctt cggccaggcg gcggttgaag gcgcgccgcg cggggctgtc ggcgagcatc
    10621 gagtccgctt cgcgctgcgg tgccatcacc tcggccgcat ccggcagggc cttttgcgcg
    10681 gcgatgaaac gccgcacatc cgcgacgaaa tccagctcct cgggcgaaaa ctcgaaatcc
    10741 acgcgattct cccgttcagg cgtcgtgctg catcaactcg atggtattgc cgtcgccgtc
    10801 ctggatctgc gcgacgcgca cccccggacg cagttgcttc ggtgccatcg gcaccctgaa
    10861 gcccgccgcg gccaccgccg cgacggtctg ctcgaggttc gcgatcgcga gcgtgaggta
    10921 gcggatgccg gtgcggctcg cgaatccgtc gccgtgctcc tgctcgacgg gcagcgtctc
    10981 gggcatgaag atgcgcagcg tgctctcgcc caccgcgtag cggcgcacca gcccgatgcc
    11041 cggaatcgcg atctcgcccg ccgccggcaa tcccagcacc tcgccataga agcgcgccgc
    11101 gcgctcgagg tcccgcgtga cgatacccgc gtccagcacg gccttctgca actgcattgc
    11161 catcgtttca gctccttctc ggttcgttgc tgtatcggcg ctagccctgg ccgcccatgg
    11221 ccgccaggtt gcgctggatg gtctcgggat cgagaccgcg atggccccag atatcgccct
    11281 cgacgaattc agacggcgtc cagtgctcat cgacgcgcac gccctcacaa ccgatctcca
    11341 cctcgaagcc gaaggggctc gccatgtaga aagacagcat gcggtcgttg gcgtggcgcc
    11401 cgagcgtgga ggtgatcggg atgccggcct cttccacgcg ctgcaggcag cggccgacca
    11461 tgtcgatgtc gcgcgcctcg agcatcaggt gatgcacgcc ggcaagatcg cccaccttca
    11521 acagtccgat cgagtgatgg cgcgcgttgc agtggaagaa gttcgccgaa tcgcccgggc
    11581 cgaagcggat gaaatcggtc aggcgaaggc ccagcacgcg cgtgtagaaa tcgcgcgcgg
    11641 ccacgagcgg cgcggccagc aggttgatgt gccccacgcc cagggtgccg gcgacgaact
    11701 ccatgcccgc gggagagacg aacttgcggt ccttgaccgg gccatagaac agctcgatgg
    11761 cattgcccat cgggtcgcgc gtgtgtccca tgccggtcac cgcgcgcgca cgcgcctcgg
    11821 cctcggtgcc ggcgcgcgcg tcgcagccgg ccgcgcgcag ctccgcgagc gcctgctcca
    11881 gctcgagcgc tcccgccact tccagcccca ggtacatcag ccccgcccgc cccgcgcgcg
    11941 ggtgcacccc gatgcgccac tgccactcgt ccagcttgag atacacgctg ccatcggccg
    12001 cgatgccact gccggcgctg gccgggccgc tgccgggcac cgcgggcatg ccccagtcct
    12061 cgccgggaca ggcgcgcgcc ggcatcaggc cgagcacgga cgttgcgtaa tcgagacagg
    12121 cccgcggatc ggggacgccg atgccgatgt aaccgagcgc acagatcttc atgcttccct
    12181 cgtcgtcgtt gtttgtaccg gcacgtgcag gaagcccgac aggtgctcgc cgtaggcacg
    12241 ctcgcactcg tcgacgagca ccgcgtagtc ccccgccgcc ttcaggaact cctcgatcac
    12301 gatcaccccg gccatcgtcg ccaggtgcat gccgaggcac ttgtggccgc cggcaccgta
    12361 ggtgagatcg cgctcgtagc ggcggaagat atcgaattcg tcggcgcgct cgaactcggc
    12421 ctcgtcgcgg ttggccgagg cgtagatgta gagcaggtcc tgcccggcct tgatgtcggc
    12481 gccgcccagc gtgaagtcgc gcaccgcgcg acggcacagc atgttggtgg gctggtcgaa
    12541 gcgcgccgtc tccaggaaga gctgctggac cagcccgtgg tccgcgagca ccgcggcctt
    12601 ctgcgccggg tgccgctcca ggtagtagag cgcgccggca cagaccatcg gcgtggtctc
    12661 ggaaccgaca atgagaaagt tcatcagcag gttgaccatg tcctggtcgt cgaggcgcct
    12721 gccgtcgatc tcggtagtca tataggccgc cgtgtggccg ctcgcgcgat cggggtggcg
    12781 gcgcagcatc tgcacgtact cggcgagata gccgccgagt tgggcggccg cgcgctggtt
    12841 gcgctccgag ctgccgcgct ggccgcgatc gcgatggatc atgtcgtcga tcagcgcgcg
    12901 ccaggtaatg gcgtcctcgc gcggcagccc gaggttgtag ccggaattga ggcagaacac
    12961 gcggttcgcg taatccgaat agagatcgaa gcgtccgcgg gccacgaggg gctccagcac
    13021 ctcccgcacc agggtccgga tgcgcgactc gtgcgcgcgc acgccctcgg gcgtgtactc
    13081 cttgcggacg accccgcgcc acttgcggtg atccggcgcg tccatcgtgg tgaaggcgtg
    13141 cggcaccggc tcgccgagca gcaactgcgt cggcagcgcc ccggcggtcc acgtgacgtc
    13201 ggcctcgtgg tgcatgctcg cttcccagac atcggcgaag cgcgtcagcg cccaggcgtt
    13261 gtactgcgga atgaagtgcg gcctgccctc ggcgcgcatc gccgcataga gggggaaggg
    13321 atcgcgcatc gcggcgtcgg agaacggatc gtagtcgaga ggcatggcag gatcccgggg
    13381 cggtgatatt tatggtactg atcgtatcgt tattatcgtc gggggtcaac cgtcagccgt
    13441 caatcgctgc gcggggtcgc cggattgcgc cgccgccact cgtccggcgt ctcgccggtc
    13501 cattcgatga aggcgcggcg gaaggagcgc acgtcgctga agccgagccg cagcgccacc
    13561 tcgctgacct tgatgcgcgc cgccgggtgc agcaattcct gcgcaagctt caggcgaacg
    13621 cgctgcttga tcgcgtcgag cgtgacatcc tcctcgcgca agcggcgctg gaatgtcgcg
    13681 cgactgagat tgaagacact cgcgaactgc tcgagcgtcg gcaggctctg ctggcgcgcg
    13741 aggcgcgtcg taatgatgtg ctcgactgcc tccgcaaact ggcctgcgtc gcgcggctcg
    13801 cgcagatggt cgaacgggaa cacgctcagg cgctccacca ggcgctggta gctgcgcacc
    13861 accggcttcg cgagcaggcg agcgggaaaa cggaagctgt tgtcggggca acccaggcgg
    13921 atcggctgct ggaaaaagcg ctccagcgtg gcgcgatcga ccagcggctc ggcgtagagg
    13981 tcatagcggc tcaccgggat gcgttcgccg atcagccacg cgaacagccg gtggtagaag
    14041 ctgagcccgt tgaggtccgt gagcagcccg ctgacgctgt ggcgcaggcg ctgcgtggcc
    14101 atgtggaaca ccgcctcgtc gccgttcaca tccagcgaaa ggtcggccgc gcggtggttg
    14161 agcatggcgc agaagcgccg cgcccgctcg atgacctctt ccagcgtggc gcaggtgatc
    14221 acgcaatagc agagcatgtc gacctcgtcc ttgctcatcg gaggcaggtc gcgctcgcga
    14281 ttggcatgcg ccgagagcac cgtgatgcac tcgccgtaga gctgcacgaa gtcatgatcc
    14341 gaaatgacct gcatccggcc agcgaccagg tcagcctgcg tgaacggcag gccgagacgc
    14401 gccagcaggc cgtccgggtc accgccgatc gctgccacct cctgcagcag cgtcagcgcg
    14461 aattgcgccg gaacgccctg cggcggatga cgcggattct gccctgccat caggccctca
    14521 ctcccgcgga tttctgcaca tccagctccc ggcgcaaccg atgatgggcg caaacttaaa
    14581 ccatctgagc tgcaatgtcc ccctgttgaa cccgttgggc ctcgaaagcg ccgggcgcgc
    14641 gcgcataatt cccgcccatg ctgtcgcgta gactcgtgac agctccagga ccaccataac
    14701 gcaccgagga aaaggcacga tgagaattcc cccacgcgaa gtccgctacg gcggcgccat
    14761 gatcgaccag aaggaaatcg acgccgtcgt caacgtgatg cagaccggca tgtccgtcgg
    14821 cacgcaggtg cagaccttcg agagccgctg cgcgaagctg ctcggcaagg aatacggcgt
    14881 gatggtgaac tccggcagct cggcgctgct ggttgccgtg cgcctgctcg acctgccgcc
    14941 cggatcggag atcatcacgc cgacgctcac cttcggcacc gacatctcct gcatcgtact
    15001 caatggccac gtgccggtgc tggtcgacgt cgagcccgac acctaccagg tcgatatcga
    15061 caagatcgaa cgcaacatca cgccgctcac caaggcgctg ctgatcccga gcctggtcgg
    15121 cggcatgccg gactgggacc gtctgcgcgc cctggccgac aagcacggcc tgaagatcat
    15181 cgaggacagc tgcgacacgc tgggcggcac ctaccgcggc acgccggccg gcaatcgcag
    15241 cgacatcagc gtgaccagct tctcgatctt ccacatcatc acctgcctgg g
//
LOCUS       DAORUQ010000066        15242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3676_length_15242_cov_7.305327, whole genome shotgun sequence.
ACCESSION   DAORUQ010000066 DAORUQ010000000
VERSION     DAORUQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15242
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3676_length_15242_cov_7.305327"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..809)
                     /locus_tag="PLW13_07655"
     CDS             complement(48..809)
                     /locus_tag="PLW13_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY70286.1"
                     /translation="MSVVDIERRGHCAVLTLNRPQVLNAINNDMLDELERFLETIRDD
                     DSRALILSGTGRAFCAGTDLSEWHGDPQHRLLRVHALVKRMLEFPKPLVAALNGLALG
                     GGLELALACTFRVARRGAKLGLPEIKLGLLPAYAGTQLLPRLIGENRALEMMLSGDAV
                     DGANALAMGLVNRICEDDADVVDAAAEFAASFTRHSLVPQRAIRRAIAEGARLPLDEA
                     LRHERELVREVSASADTLEGVQAFLEKRAPRWRDM"
     gene            936..1787
                     /locus_tag="PLW13_07660"
     CDS             936..1787
                     /locus_tag="PLW13_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQY70287.1"
                     /translation="MASLPRLAVREPVERIHAALDEHGAVIVEGLLDQPLLARLNGDV
                     DPLLARADPHMNHLNPVLDAFFGTCVRHVTGLAGKSLAFAENVMCHPLLLGLCDRVLL
                     PGCAGYQLNLGHLMDRGPGAEAQWPHRDEDVWVHCPRPHPELQLATVVALVDFHRGNG
                     ATYVVPGSHRWPRDRVATEAETAHAEMPAGSAVIYLGSTIHGGGANSTADDWRRGFHL
                     SYTLGWLRTEENNILAVPPERARELSPLARRLVGYGVHDAIADMGGYLGMLDMRDPAQ
                     MLEDGSL"
     gene            complement(1826..3616)
                     /locus_tag="PLW13_07665"
     CDS             complement(1826..3616)
                     /locus_tag="PLW13_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY70288.1"
                     /translation="MSTILSLDNAELAYGLHPLLDRASLTVTQGERIGLIGRNGTGKS
                     SLLRVIAGDAPLDDGELRLPAGGRVILVEQEPELPPAATLRESLVLRGGFDAIHDDKE
                     RWRLEARLSEYAHRLQLNEYADMARLSGGERKRAALALALALEPELLLLDEPTNHLDI
                     EGITLLEELLAKVSAAIVITHDRAFLDRIATRIVELDRGLLRSYPGNFSAYESRKAEQ
                     LAAEAVAARKFDKFWAEEEAWIRQGVKARRTRNEGRVRRLERLRNERAERRERMGNVR
                     LRLDAGERSGKLVAELENVSKSFGERTVVRDLSLRLLRGDRLGLMGPNGAGKSTLIKL
                     ILGQMQPDGGTVKLGTSLEVAYFDQMREQLDLAKTVAETISPGSEWVEIGGARKHIIS
                     YLGDFLFPPQRAGSPVGTLSGGERNRLLLARLFARPANLLVLDEPTNDLDIESLELLE
                     DTLQGYTGTLLLVSHDRVFLDNVVTQTLVAEGDGHWQEYAGGYSDWLLQRPQPVAAPA
                     PVATPAAAKAPAPGRAVAKKLSFKEQRELESLPATIDALEQEQEALLASMGSGNVEAM
                     RAASTRAAEITELLESAFARWSELEDRASH"
     gene            complement(3745..4176)
                     /locus_tag="PLW13_07670"
     CDS             complement(3745..4176)
                     /locus_tag="PLW13_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HQY70289.1"
                     /translation="MSRYLAALRPLVLVAAALGAAAAFAETPSQPEAVAYFISPADGA
                     TVANPVTVRFGLRGMGVAPAGVERPDTGHHHLLIDLDTLPPAGMPMPMDEQHRHFGGG
                     QTEVTLELAPGKHTLQLLLGDHMHVPHNPPVVSERITITVE"
     gene            4321..6492
                     /gene="uvrD"
                     /locus_tag="PLW13_07675"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             4321..6492
                     /gene="uvrD"
                     /locus_tag="PLW13_07675"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HQY70290.1"
                     /translation="MYAESILDSLNTAQREAVTAPDTNLLVLAGAGSGKTRVLVHRIA
                     WLVFQDGISPHGIMAVTFTNKAAREMRARIEEMVGVSTRGMWVGTFHGLAHRFLKAHW
                     EEAGLPQNFQILDSDDQLRVIKRVYRDTGIDEDRFAPKQSQWWINAQKDEGRRAAHIE
                     SYGDHYLNTMLVLYRGYEQACERGGMVDFAELLLRSHELWLKQPALLAHYQQRFRHIL
                     VDEFQDTNTIQYAWLRVLAGKAARVTAVGDDDQSIYGWRGAKIENIHQFQRDFDDTRI
                     VRLEQNYRSTSVILDAANAVIAHNQGRFGKQLWTSGEEGEPITLYAAFNEQDEARFIV
                     DHIQRWIADGHPRADAAILYRSNAQSRVLEEALLRSGIPYRVYGGQRFYERLEIRNAL
                     AYLRLITNRHDDAAFERVVNTPTRGIGAATIDLLRASARERGISLWAAAAQVAAGLPA
                     RARNALTAFLDLIDQLQRDTAGMDLAELADHVLAASTLLEFHRNEKGERGQTRAENLE
                     ELVSACRQFVPENPEQPPLPQFLDRASLDAGDGQAEEFEDGVQLMTLHSAKGLEFPLV
                     FLAGMEENLFPHAMSREEPGRLEEERRLAYVGITRAMRSLFLTYAESRRLHGNETFNS
                     MSRFVREIPPQLLREVRPRASITRPVSLADQYRAPSAATRGAEVPQTGLRLGQAVRHP
                     KFGEGVIINFEGQGAHARINVNFAREGAKWLVLSYANLEPA"
     gene            complement(6513..6947)
                     /locus_tag="PLW13_07680"
     CDS             complement(6513..6947)
                     /locus_tag="PLW13_07680"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQY70291.1"
                     /translation="MKTPVKTRTVSAIDEAPRPSGELALQTIAMPKDTNANGDIFGGW
                     LLSQMDLAGGIAAAKVARGRVATVAIDRMSFMVPVQVGAVVSCYTEVISVGRSSIQVQ
                     IEVWSRVSGSTGETGSHDKVTEGTFVFVAIDENGRTRAVPTS"
     gene            complement(6963..8258)
                     /locus_tag="PLW13_07685"
     CDS             complement(6963..8258)
                     /locus_tag="PLW13_07685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY70292.1"
                     /translation="MNALDPLRSALAVLVLGGALGGCGVNPVTGSSEMSFVSEQQEIR
                     TGEQQYGPSQQSEGGQYVLDPELNAYVARVGQSLARVSERSQLPYEFVVLNNSSPNAW
                     ALPGGKIAVNRGLLVQLEDESQLAAVLGHEIVHAAARHGAKAQETGTLLNIGLAVLGV
                     ATANSAYQDLALQGAQISGALVQTRYGREAELESDYYGMQYMARAGYDPQGAVRLQET
                     FVKLSQGRRQDWLSGLFASHPPSQERVDANRRTAQKLGPGDDGREEYQRRIARLKRDQ
                     PAYDAYDRGVAAMNAGRYQEALKYAEQALKLQEREGLFHELRGVAAARLDRPADAINS
                     FNRAIAANPNFFRPHLLRGKLHLERGNLDIAAQDLNASNRLLPTADATFGLGEIAQRQ
                     GDTQTALRHYQAVANSGSSLAPAARERIARLGSGLTNIR"
     gene            8354..9118
                     /gene="yaaA"
                     /locus_tag="PLW13_07690"
     CDS             8354..9118
                     /gene="yaaA"
                     /locus_tag="PLW13_07690"
                     /inference="COORDINATES: protein motif:HMM:NF002542.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HQY70293.1"
                     /translation="MLLIVSPAKTLDFDSPVPELTVTRPAFTKDAVALIDGLRALPVE
                     AIRNLMSISPELAALNHARYRDWRARAPARATRAALLAFRGDVYLGMRAETFSAEEFA
                     FAQQHLRILSGLYGLLRPLDAIQPYRLEMGTQLVNARGRNLYEFWGPRIADALQVQAR
                     AAGARELVNLASQEYFRAVAQERLTLAVITPVFKESRGGTLKIVSFNAKRARGAMAGF
                     AIRHRLQRAEDLKAFAEDGYRYEPALSCDREWLFVR"
     gene            9198..9794
                     /locus_tag="PLW13_07695"
     CDS             9198..9794
                     /locus_tag="PLW13_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HQY70294.1"
                     /translation="MTELPFSEACERNKDPILEKLRVHFAGATRVLEIGSGTGQHALH
                     FAAALPQLVWQTSEVPGQDEAVRARLAAHPLPNVRAPLALDVRGAWPAGPFDAIFSAN
                     TLHIMGWDGVSALFSGVGQCLASGGVLAIYGPFNYGGRYTSDSNAAFDDWLRRRDPAS
                     GIRDHEAVDALARAAGLEPVADHAMPANNRLLVWVRAP"
     gene            9791..10207
                     /locus_tag="PLW13_07700"
     CDS             9791..10207
                     /locus_tag="PLW13_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HQY70295.1"
                     /translation="MTLQRFLDAQHAVYAQALEELRAGRKRTHWMWFVFPQLAGLGLS
                     ATAVHYAIADLAEARAYLAHPLLGPRLEECARALLDVPGRTAHEILGSPDDLKLRSSM
                     TLFAQVDGADPVFRRVLERYYDGEADARTLALLASS"
     gene            complement(10236..11378)
                     /locus_tag="PLW13_07705"
     CDS             complement(10236..11378)
                     /locus_tag="PLW13_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY70296.1"
                     /translation="MTRAQFLPGIREVAAADWNALARRDYPFLRHEFLCALEDSASVG
                     GDSGWEPHHLLLREGARLVGAMPCYLKRHSYGEYVFDWSWADAYRRHGQPYYPKLLSA
                     VPFTPAAGPRLLLAEGLAFADLQAELLAAVREEMARLGASSWHLLFPEQPVAGELAAA
                     GLGHRTGVQFHWHNRGYRDFADFLDLMQSRKRKQLKRERRLVVEQGLQLRALRGTEVD
                     AALWDRFHHFYQLTYARRSGHGGYLNRAFFRLLGQSMPERLLLVVAEHRREVVAGALY
                     VIGADTLYGRYWGCTEDYAQLHFETCYYQGIDFCISEGLARFDAGAQGEHKLARGFEP
                     VLTHSCHLIAHPGFRAAIDDFLLRERDAIESYRREAAAALPYRDAV"
     gene            complement(11375..12238)
                     /gene="ubiA"
                     /locus_tag="PLW13_07710"
     CDS             complement(11375..12238)
                     /gene="ubiA"
                     /locus_tag="PLW13_07710"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQY70297.1"
                     /translation="MELRARLPLYLRLIRFDRPIGTFLLLWPTWWGLWFAAQGQPSLA
                     NLLIFTAGVFLMRSAGCVANDYADRHIDGHVERTRERPLASGQLGEREALALGAVLAL
                     ASFALVLLTNALTVWLSFAALVFAAGYPFLKRYTHLPQVGLGIAFSWGIPMAFAAERQ
                     VLSPPLWLLFFAAVLWSVVYDTFYAMVDREDDLAIGVKSTAILFGDADRLVTALLQFL
                     VLVLLWLAGRQFGMGLPYTLALLGGAALFAWQQYLIRERERARCFRAFLNNNLFGLLV
                     FAGIATDYLLR"
     gene            complement(12270..12836)
                     /locus_tag="PLW13_07715"
     CDS             complement(12270..12836)
                     /locus_tag="PLW13_07715"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HQY70298.1"
                     /translation="MYALASAGFQPGEPAWKPWRRFDSSQLPPATRHWLLDEGSLTAI
                     LQAACGNRLRVRVLRQAWQRPRASERRALGLGAHEVALVREVLLECAGEPWVFARSIM
                     PAATLRGRLRHLRRFGERSLGALLFSSRGLRREPFEVALVRAGHGILPAPPADGATVW
                     GRRSVFRVHGAPLLVAEFFLPACRLGSL"
     gene            12976..14349
                     /locus_tag="PLW13_07720"
     CDS             12976..14349
                     /locus_tag="PLW13_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70299.1"
                     /translation="MARWECIVCGWVYDEAKGWPDDGIAAGTRWEDVPNDWLCPDCGV
                     GKEDFELIDDTPLVAVPHHQEADIVSLTQPVIIIGTGLAGYNLAREFRKLDSDTPLIL
                     VTSDDGRMYSKPMLSTGFTKNTTPDDLATMDAGSMAISLRASVWTHTHVTAIDTAKHE
                     IRIGEATTVGYSRLVIAWGADTIRPPLKGDGLDHVYSVNDLLDYADFRAAIRNNDVKK
                     VLIIGAGLIGCEFTNDLCNGGFAVEAVDPLGWCLPTLLPEPAGRAVQGALEALGAKFH
                     FGPLVSEVNLAPGGKGIVATLNDGRRIEADMAVSAIGVRPRLELAKAAGIAVNRGVVA
                     NRLLATSAPDVYVLGDCAEVDGHVLYYVAPLMACARALAKTLAGTPTEVSYPAMPVTI
                     KTPACPVVVAPPPVGAAGAWSISGSSPDLVAEFRDPAGKLLGFALTGEGGKQKLQLQK
                     ELPPIIA"
     gene            complement(14358..>15242)
                     /locus_tag="PLW13_07725"
     CDS             complement(14358..>15242)
                     /locus_tag="PLW13_07725"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQY70300.1"
                     /translation="IGNAVARFTARRIERRLEDTLDFPPLPEIAHRIIRISADPYASV
                     SDLARVVEIDPSLSAQVVSWASSPYYAAPGKVKSIQDAIVRVLGFDLVMNLALGLALG
                     KTMRLPKEGRYGYRNYWLQAVHGAAMVEGLCKAMPSSLRPALGHAYLGGLLHNFGFLI
                     LAELFKPQLQLVCRYTEANPHLGHWHVERFLLGVTREQMAGWLMRFWHLPEEVCAALR
                     FQQAPEQAGEHEVLARVLHLATRLLREQHIGSAPLQPIADSMFTELGISRADALAVSE
                     RIHGSAGELQAIARDLAA"
BASE COUNT         2347 a         5272 c         5288 g         2335 t
ORIGIN      
        1 aggtccggct tcagccggac attacatctg tccggctgaa gccggaccta catgtcccgc
       61 cagcgaggcg cgcgcttttc gaggaaggcc tgtacgcctt cgagcgtgtc ggcgctggcg
      121 gagacctcgc gcaccagctc gcgttcgtgc cgcagcgcct cgtcgagcgg caaccgcgcg
      181 ccttcggcga tggcgcgccg gatcgcccgc tgcggcacca ggctgtgacg cgtgaagctc
      241 gcggcgaatt cggccgccgc gtccaccacg tccgcgtcgt cctcgcagat gcggttcacc
      301 aggcccatcg cgagcgcgtt cgcgccgtcc acggcatcgc cgctcagcat catttccagc
      361 gcgcggttct cgccaatcag ccgcggcaac agctgcgtgc cggcgtacgc gggcagcagg
      421 ccgagcttga tctcgggcaa cccgagcttc gccccgcgcc gcgccacgcg gaaggtacag
      481 gccagcgcca gctcgaggcc gccgccgagc gccagcccgt tcagcgcggc gacgagcggt
      541 ttcgggaatt cgagcatgcg ttttaccagc gcatgcacgc gcagcagccg atgctgcgga
      601 tcgccgtgcc attcgctgag gtcggtgccg gcgcagaagg cgcgcccggt gccgctcagg
      661 atcagtgcgc ggctgtcgtc gtcgcggatc gtctcgagaa aacgttcgag ctcgtccagc
      721 atgtcgttgt tgatggcgtt cagcacctgt ggccggttca gggtcaatac cgcgcagtgt
      781 ccgcgccgtt cgatgtccac cacgctcatg ccgtcgtctc cgtgttcgcc tgcgccgcaa
      841 acgttccggc gcccggcgcg actataggag aattcgcgcg cctggcgtag ccttgctgca
      901 attgccaccg atcgcatacc cgggagtgat gcaccatggc cagcctgccg cgacttgccg
      961 tccgcgagcc cgtcgagcgc atccacgccg ccctcgacga gcacggtgcg gtgatcgtcg
     1021 agggcctgct cgatcagccc ctgctcgcgc ggctcaacgg cgatgtcgat ccgttgctgg
     1081 cgcgcgccga tccgcacatg aaccatctca acccggtgct ggacgcgttc ttcggcacgt
     1141 gcgtgcgcca cgtgaccggg ctcgccggca agtcgctcgc cttcgccgaa aacgtgatgt
     1201 gccatccgct gttgctggga ctctgtgacc gcgtgctgct gccgggctgt gcgggctacc
     1261 agctgaacct gggccacctg atggaccgcg gccctggtgc cgaggcgcag tggccgcatc
     1321 gcgacgagga cgtgtgggtg cattgcccgc gaccgcaccc ggagttgcag ctcgcgaccg
     1381 tggtcgcact ggtggatttc caccgtggca acggcgcgac ctacgtggtg cccggcagcc
     1441 atcgctggcc gcgcgatcgc gtcgccaccg aggcggaaac ggcgcacgcc gagatgccgg
     1501 ccggttcggc ggtgatctac ctcggctcga cgatccacgg cggcggtgcc aacagcaccg
     1561 ccgacgattg gcgtcgtggc ttccacctca gctacaccct gggctggctg cgcaccgagg
     1621 agaacaacat cctcgcggtg ccgccggaac gcgcgcgcga gctctctccg ctggcgcggc
     1681 gcctcgttgg ctacggcgtg catgacgcca tcgccgacat gggcggctat ctcgggatgc
     1741 tcgatatgcg cgatcccgcg cagatgctgg aagacggctc gctctaggca cgccccgcgt
     1801 cacgccgcat cgcagggggg cgtcatcagt gcgaggcgcg gtcctcgagc tcgctccagc
     1861 gcgcgaaggc ggattcgagt agttccgtga tctccgccgc gcgcgtcgac gcggcgcgca
     1921 ttgcctccac gttgccgctg cccatgctcg cgagcaacgc ttcctgttcc tgctccagcg
     1981 cgtcgatggt ggcgggcagg ctctcgagct cgcgctgctc cttgaagctc agcttcttcg
     2041 cgacggcgcg tccgggcgcg ggcgccttcg ccgcggcggg agtcgcgacc ggcgccggcg
     2101 ccgcgacggg ctgtggccgt tgcagcagcc agtcgctgta gccaccggca tactcctgcc
     2161 agtggccgtc gccttcggcg accagcgtct gcgtcaccac gttgtcgagg aacacgcggt
     2221 cgtggctcac cagcagcagc gtgccggtgt agccctgcag cgtgtcctcg agcagttcca
     2281 gcgactcgat gtcgaggtcg ttggtcggct cgtccagcac cagcaggttt gccgggcgcg
     2341 cgaacagccg cgccagcagc aggcggttgc gctcgccgcc cgagagcgtg ccgaccggcg
     2401 agccggcgcg ctgtggcgga aacaggaagt cgccgaggta gctgatgatg tgcttgcgcg
     2461 cgccgccgat ctccacccac tccgagcccg ggctgatggt ctcggcgacg gtcttcgcca
     2521 ggtcgagctg ctcgcgcatc tggtcgaagt aggccacctc gaggctggtg cccagcttca
     2581 cggtgccgcc gtcgggctgc atctgcccga gtatcagctt gatcagcgtg ctcttgccgg
     2641 cgccgttcgg tcccatcagc ccgaggcggt cgccgcgcag cagccgcagc gacaggtccc
     2701 gcaccacggt gcgctcgccg aaggacttcg agacgttttc cagctcggcg accagcttgc
     2761 ccgagcgctc gccggcatcg agacgcagtc gcacgttgcc catgcgctcg cgccgttccg
     2821 cgcgctcgtt gcgcaggcgt tccagccggc gcacgcgccc ctcgttgcgc gtgcgccgcg
     2881 ccttgacgcc ctggcggatc cacgcttctt cctcggccca gaacttgtcg aacttgcgtg
     2941 ccgccacggc ctcggccgcg agctgctccg ccttgcgcga ctcgtaggcc gagaaattgc
     3001 cgggatagga gcgcagcagg ccgcggtcga gctcgacgat gcgggtcgcg atgcggtcga
     3061 ggaaggcgcg gtcgtgagtg atcacgatcg ccgcggacac tttcgcgagc aattcctcga
     3121 gcagcgtgat gccctcgatg tcgaggtggt tcgtcggttc gtccagaagc agcagctccg
     3181 gctccagcgc cagcgcaagg gccagcgcgg cgcgcttgcg ctcgccgccg gagagccgcg
     3241 ccatgtcggc gtactcgttc agttgcagcc ggtgcgcgta ttcgctcagc cgcgcctcca
     3301 gccgccagcg ttccttgtcg tcgtggatgg cgtcgaagcc gccgcgcagc accaggctct
     3361 cgcgcagcgt cgcagcgggt ggcagctcgg gctcctgctc gaccaggatc acgcgaccgc
     3421 cggcgggcag gcgcagctca ccgtcgtcga gcggggcgtc gcccgcgatc acgcgcagca
     3481 gcgaggactt gccggtgccg ttgcgtccga tcagcccgat gcgctctccc tgggtgacgg
     3541 ttagcgaggc gcggtcgagc agcgggtgca gaccgtaggc gagttcggcg ttgtcgagac
     3601 tgaggatggt gctcatggcg atacgtacgg cagatgcgaa ggggatcggg tcggcgcggg
     3661 caggtgcagc gcgagcggct gcccggcggc gcgcacgctg gcggcgccgt cgggcttcag
     3721 ggacaggggc ttacggcccc actgtcattc gaccgtgatg gtaatgcgct ccgagaccac
     3781 cggcgggttg tgcggcacgt gcatgtggtc gcccagcagc agctgcagcg tgtgcttgcc
     3841 cggtgcgagc tccagcgtca cctcggtctg cccaccgccg aaatgccggt gctgctcgtc
     3901 catcggcatc ggcatgccgg cgggcggcag cgtgtccagg tcgatcaaca ggtgatggtg
     3961 tccggtgtcg ggacgctcca caccggccgg cgccacgccc atgccgcgca gcccgaagcg
     4021 cacggtgacg gggttggcga cggtcgcgcc atcggcgggc gagatgaaat aggcgaccgc
     4081 ctcgggttgc gacggcgttt cggcaaacgc ggcggcggcg ccgagcgcgg cggcgaccaa
     4141 aaccaggggt cgcaatgcgg cgagatagcg ggacatcgtg atgctccggg tccagtgacg
     4201 acgggtgagc ggcggagcat aacaaaaagc ccgccccggc aaacgggcca gggtacaaat
     4261 acggcgtgcg cccgggaagc cgcgggaccg gttgcaggcg attgttggta gaatccgccg
     4321 atgtacgccg aaagcatcct cgactccctc aacaccgcgc agcgcgaggc cgtcaccgcg
     4381 ccggatacca atctgctggt actggccggc gccggcagcg gcaagacgcg cgtgctggtg
     4441 caccgcatcg cgtggctggt attccaggac ggcatctcgc cgcacgggat catggccgtg
     4501 accttcacca acaaggccgc gcgcgagatg cgcgcgcgca tcgaggagat ggtcggggtc
     4561 tcgacgcgcg gcatgtgggt gggcaccttc cacgggttgg cgcaccgctt cctgaaggcg
     4621 cactgggagg aggccgggct gccgcagaac ttccagatcc tcgattccga cgaccagttg
     4681 cgcgtgatca agcgcgtcta ccgcgacacc ggcatcgacg aggaccgctt tgcgcccaag
     4741 cagtcgcagt ggtggatcaa cgcgcagaag gacgagggcc ggcgggccgc gcacatcgag
     4801 tcctacggcg accattacct gaacacgatg ctggtgctct accgcggcta cgagcaggct
     4861 tgcgagcgcg gcggcatggt ggacttcgcg gaactgctgc tgcgctcgca cgagctgtgg
     4921 ctgaagcagc cggcgctgct ggcgcactac cagcagcgct tccggcatat cctcgtcgac
     4981 gagttccagg acaccaacac gatccagtac gcctggctgc gcgtgctggc gggcaaggcc
     5041 gcgcgcgtca ccgcggtcgg cgacgacgac cagtcgatct acggctggcg tggcgcgaag
     5101 atcgagaaca tccaccagtt ccagcgcgac ttcgacgaca cgcgcatcgt gcgcctggag
     5161 cagaactacc gctcgaccag cgtgatcctc gacgcggcga acgcggtgat cgcgcacaac
     5221 cagggccgct tcggcaagca gctgtggacc agcggcgagg aaggcgagcc gatcacgctc
     5281 tacgccgcgt tcaacgagca ggacgaggcg cgcttcatcg tcgatcacat ccagcgctgg
     5341 atcgccgacg gacacccgcg cgccgatgcc gcgatcctct accgttccaa cgcgcagtcg
     5401 cgggtgctag aggaggcgct gttgcgcagc ggcattccct accgcgtcta cggcggccag
     5461 cgcttctacg agcgtctcga gatcaggaac gcgctggcat acctgcgcct gatcaccaac
     5521 cgtcacgacg acgcggcctt cgagcgcgtc gtgaacacgc cgacgcgcgg catcggtgcg
     5581 gcgaccatcg acctgctgcg cgcgagcgcg cgcgagcgcg gcatctcgct gtgggccgcg
     5641 gccgcgcagg tcgccgccgg gctgccggcg cgcgcgcgca atgcgctcac cgcgttcctc
     5701 gatctgatcg accagctgca gcgtgatacc gccggcatgg acctggccga actggccgac
     5761 cacgtgctcg cggccagcac gctgctcgag ttccaccgta acgagaaggg cgagcgcggc
     5821 cagacgcgcg ccgagaacct cgaggagctg gtgagcgcct gccgccagtt cgtgcccgag
     5881 aacccggagc agccgccgct gccgcagttc ctcgaccgcg cctcgctgga tgccggcgac
     5941 ggccaggccg aggagttcga ggacggggtg caactgatga cgctgcactc ggcgaaaggc
     6001 ctggagttcc cgctggtgtt tctcgcgggc atggaggaaa acctgttccc gcacgcgatg
     6061 tcgcgcgagg agccgggccg gctggaggag gaacgccgcc tcgcctacgt gggcatcacg
     6121 cgcgcgatgc gctcgctgtt cctgacctac gccgaatcac gccgcctgca cggcaacgaa
     6181 accttcaact cgatgtcgcg cttcgtgcgc gagatcccgc cgcagctgct gcgcgaggtg
     6241 cgtccgcgcg cctcgatcac gcgcccggta tcgctggccg accagtaccg cgcgccctcc
     6301 gccgccacgc gcggcgccga ggtgccgcag accggcctgc gcctggggca ggcggtgcgt
     6361 cacccgaagt tcggcgaggg cgtgatcatc aatttcgagg gccagggcgc acacgcgcgc
     6421 atcaacgtga acttcgcgcg cgaaggcgcg aagtggctgg tgctgtccta cgccaatctc
     6481 gagcccgcct gaacaaactt ccagcggcag cctcaggacg tgggcactgc ccgcgtgcgg
     6541 ccgttctcgt cgatcgcgac gaacacgaag gtgccctcgg tcaccttgtc gtgcgagccg
     6601 gtttcgcccg tgctgcccga gacgcgtgac cacacctcga tctgcacctg gatcgagctg
     6661 cgcccgaccg agatcacctc ggtgtagcaa ctcacgacgg cgcccacctg caccggcacc
     6721 atgaagctca tgcggtcgat ggcgacggtg gccacgcgcc cgcgcgcgac cttcgcggcc
     6781 gcgataccgc ccgcgagatc catctgcgac agcagccagc cgccgaagat gtcgccgttg
     6841 gcgttggtgt ccttgggcat cgcgatggtc tgcagggcga gctcgccgct cggtcgcggg
     6901 gcttcgtcga tagcggatac ggttcttgtc tttacgggag tcttcatggg aatgtccggt
     6961 cgtcagcgaa tgttggtgag gccgcttccg aggcgggcga tgcgctcgcg cgcggcgggc
     7021 gccagcgatg agccgctgtt ggccaccgcc tggtaatggc gcagcgcagt ctgggtatca
     7081 ccctggcgtt gcgcgatctc gccgagcccg aaggtcgcat ccgccgtggg cagcagccgg
     7141 ttcgaggcgt tcaggtcctg tgcggcgatg tcgagattgc cgcgctcgag gtgcagcttg
     7201 ccgcgcaaca ggtgggggcg gaagaagttc gggttcgcgg cgatggcgcg gttgaagctg
     7261 ttgatcgcgt cggccgggcg atcgagtctc gccgcggcga cgccgcgcaa ttcgtggaac
     7321 agcccttcgc gctcctgcag cttcagcgcc tgctcggcgt atttgagggc ctcctggtag
     7381 cgcccggcgt tcatcgcggc cacgcctctg tcgtaggcgt cgtaggccgg ctggtcgcgc
     7441 ttcaggcgcg cgatgcggcg ctggtattcc tcgcgcccgt catcgcccgg ccccagcttc
     7501 tgcgcggtgc gccggttggc gtcgacgcgt tcctgcgagg gcgggtgact cgcgaacagg
     7561 ccgctcagcc agtcctgtcg tcgtccctgc gacagcttca cgaaggtttc ctgcaatctc
     7621 accgcgccct gcgggtcata gccggcgcgc gccatgtact gcatgccgta gtaatccgat
     7681 tccagttccg cctcgcggcc gtagcgtgtc tggaccagcg cgccgctgat ctgcgctccc
     7741 tgcagcgcga ggtcctggta ggcactgttc gccgtcgcga cgccgagtac cgcgaggccg
     7801 atgttgagca gcgtgccggt ctcctgggct ttcgcgccgt ggcgcgccgc ggcgtgcacg
     7861 atctcgtggc ccagcaccgc ggcgagctgc gattcgtcct cgagctgcac cagcaggccg
     7921 cggttgaccg cgatcttgcc cccgggcagc gcccaggcgt tgggcgagga attgttcagc
     7981 accacgaact cgtagggcag ctgcgagcgc tcgctgacgc gcgccaggct ctgtccgacg
     8041 cgcgccacgt aggcattcag ctcgggatcg agcacgtact ggccgccctc gctctgctgg
     8101 ctgggcccgt actgctgctc gccggtgcgg atctcctgct gttccgatac gaagctcatt
     8161 tccgacgagc cggtgaccgg gttgacgccg caaccgccca gagcgccacc cagcaccagg
     8221 acggccagcg cggaacgcaa tgggtcgagg gcattcattg ggatagaatc gccgtgtggg
     8281 tggagtcggc gcgattctat cggcagagtc cgcatccgca ccacccgaat attcccgagc
     8341 cccaggaccg tgcatgctgc tgatcgtatc gcccgccaag acgctcgact tcgattcccc
     8401 cgtacccgag ctcactgtca cgcgccccgc attcaccaag gacgcggtag cgctgatcga
     8461 cgggttgcgc gcgctaccgg tcgaggcgat ccgcaatctg atgtcgatca gcccggagct
     8521 cgcagcgctg aaccacgcgc gctaccggga ttggcgcgca cgtgcgccgg cgcgcgccac
     8581 ccgtgccgcg ctgctcgcct tccgcggcga cgtctacctc ggcatgcgcg ccgagacctt
     8641 ttccgccgag gagttcgcgt tcgcgcagca gcacctgcgc atcctgagcg gcctctacgg
     8701 gttgctgcgc ccgctcgatg cgatccagcc ttatcggctc gagatgggca cgcagctggt
     8761 gaacgcgcgc gggcgcaacc tctacgaatt ctgggggccg cgcatcgccg acgcgctgca
     8821 ggtgcaggcc cgcgccgcgg gcgcccgcga actggtgaac ctggcctcgc aggagtattt
     8881 tcgcgccgtc gcccaggagc ggctgacgct ggcggtgatc acgccggtgt tcaaggaaag
     8941 ccgcggcggc acgctgaaga tcgtgagctt caacgccaag cgcgcgcgcg gcgcgatggc
     9001 cggttttgcg attcgccacc gcctgcagcg cgccgaggat ctcaaggcct tcgccgagga
     9061 cggctatcgc tacgagccgg cattgtcctg cgaccgggaa tggctgttcg tccgctgata
     9121 ctcgcgatct cgtaggtccg cattcatgcg gactgccgcc tcggcgcagc atctctgcca
     9181 gaccgacagg agcgaccatg accgaattgc ctttctctga ggcctgcgag cgcaacaagg
     9241 atccgatcct cgagaagctg cgcgtgcatt tcgccggcgc cacgcgggtg ctcgagatcg
     9301 gcagcggcac cgggcagcat gcgctgcatt tcgccgccgc gctgccgcaa ctcgtgtggc
     9361 agaccagcga ggtgccggga caggacgagg cagtgcgcgc ccggctcgcc gcgcacccgc
     9421 tgccaaacgt gcgggcgccg ctcgcgctcg atgtgcgcgg cgcgtggcct gccggtccct
     9481 tcgacgcgat attcagcgcc aatactttgc acatcatggg ctgggacggc gtgagcgcgc
     9541 tgttcagcgg cgtggggcaa tgcctggcgt cgggtggggt gctcgcgatc tacggcccct
     9601 tcaattacgg agggcgctac accagcgaca gcaacgcggc cttcgacgac tggctgcgcc
     9661 ggcgcgatcc cgcgagcggt atccgcgacc acgaggcggt ggacgcgctg gcgcgcgccg
     9721 cgggtctcga accggttgcc gatcacgcga tgccggccaa caaccgtctg ttagtgtggg
     9781 ttcgcgcgcc atgaccctgc aacgctttct cgatgcgcag cacgccgtct acgcgcaggc
     9841 gctggaggag ttgcgcgccg ggcgcaaacg cacgcactgg atgtggttcg tgtttccgca
     9901 gctcgcgggg ctgggtctca gcgcgacggc ggtgcattac gcgatcgccg atctcgccga
     9961 ggctcgcgcc tatctcgcgc acccgttgct cgggccgcgg ctcgaggaat gcgcccgcgc
    10021 gctgctcgac gtgcccggac gcacggcgca cgagatcctc ggctcgcccg acgacctgaa
    10081 gctgcgctcc tcgatgacgc tgttcgcgca agtggacggc gcggacccgg tgttccggcg
    10141 cgtgctcgaa cgctattacg acggtgaagc ggacgcccgc accttggcgc tgctggcctc
    10201 gtcctgagca cgcccgcccc gacgctgacg ccgcgctaca ccgcatcgcg gtacggcagt
    10261 gctgcggcgg cctcgcggcg gtaactctcg atcgcgtccc gttcgcgcag gaggaagtcg
    10321 tcgatcgccg cgcggaagcc cgggtgcgcg atcaggtggc aggagtgcgt gagcaccggt
    10381 tcgaagccgc gcgcgagctt gtgctcgccc tgcgcgccgg cgtcgaagcg cgccaggccc
    10441 tcgctaatgc agaagtcgat gccctggtag tagcaggtct cgaagtgcag ctgcgcgtag
    10501 tcctccgtgc agccccagta gcggccgtag agcgtgtcgg cgccgatcac gtagagcgcg
    10561 ccggccacga cctcgcgccg gtgctcggcg acgacgagca gcaggcgctc gggcatcgac
    10621 tgtcccagca gccggaagaa cgcgcggttc aggtagccgc cgtggccgct gcgccgtgcg
    10681 taggtcagct ggtagaagtg atggaagcga tcccacagcg cagcgtcgac ctccgtgccg
    10741 cgcagcgcgc gcagctgcag tccctgttcc accaccagcc gccgttcgcg cttcagctgc
    10801 ttgcgcttgc gtgactgcat caggtcgagg aaatcggcga agtcgcgata gccgcggttg
    10861 tgccagtgga actgcacgcc ggtgcgatgg ccgaggccgg cggcggccag ttccccggca
    10921 accggttgct cgggaaacag caggtgccat gacgaggcgc ccagacgcgc catttcctcg
    10981 cgcaccgccg cgagcagttc cgcctgcagg tccgcgaacg ccagcccctc ggccagcagc
    11041 agccgcggcc ccgcggccgg tgtgaacggc accgcgctca gcagcttcgg gtaatacggc
    11101 tggccgtggc ggcgataggc atccgcccac gaccagtcga acacgtactc gccgtaggag
    11161 tgccgcttca gatagcaggg catcgcgccc accagccgcg caccctcgcg cagcagcagg
    11221 tggtgcggtt cccagccgga gtcgccgccc acgctggcgc tgtcctcgag cgcgcagagg
    11281 aactcgtggc gcaggaaggg atagtcgcgc ctggcgagcg cattccagtc cgccgccgcg
    11341 acctcgcgga tgccgggcag gaactgcgcg cgtgtcatcg cagcaggtag tcggtggcga
    11401 tgccggcgaa tacgagcagg ccgaacaggt tgttgttcag gaaggcgcgg aagcagcgcg
    11461 cgcgctcgcg ctcacggatc aggtactgct gccacgcgaa cagcgccgcg ccgccgagca
    11521 gggccagcgt gtacggcaac cccatgccga actggcgacc ggcgagccac agcagcacca
    11581 gcaccaggaa ctgcagcagt gcggtgacca gccggtcggc gtcgccgaac aggatcgcgg
    11641 tggatttcac cccgatcgcg aggtcgtcct cgcgatcgac catcgcgtaa aaggtgtcgt
    11701 agaccacgct ccacagcacg gccgcgaaga acagcagcca gagcggtgga gacagcacct
    11761 ggcgctcggc ggcgaacgcc atcggtatgc cccaggagaa cgcgatgccg agtccgacct
    11821 ggggcaggtg cgtgtagcgc ttcaggaagg gatagcccgc cgcgaacacc agcgcggcga
    11881 acgaaagcca caccgtgagc gcattggtca acagcaccag cgcgaaggac gccagcgcga
    11941 gcaccgcgcc gagcgcgagc gcctcgcgct cgcccagctg tccgctcgcc agcgggcgct
    12001 cgcgcgtgcg ctcgacgtgg ccgtcgatat gccgatccgc gtaatcgttg gcgacgcagc
    12061 cggccgagcg catcaggaac acgccggcgg taaaaatgag caggttggcc agcgacggtt
    12121 gcccctgcgc cgcgaaccac agcccccacc acgtcggcca cagcagcagg aacgtgccga
    12181 tcggacggtc gaagcggatc aggcgaaggt acagcggcag gcgtgcgcgc aattccatgg
    12241 attgacctgt gaggggcgct gggtcgggat tatagcgagc ccagccggca ggccggcagg
    12301 aagaactccg cgaccagcag tggcgcgcca tgcacccgga acaccgagcg ccggccccac
    12361 accgtggcgc cgtcggccgg cggcgcgggc aggatgccgt ggccagcgcg caccagcgcc
    12421 acctcgaagg gctcgcgccg cagcccgcgg ctggagaaca gcagcgcgcc cagcgagcgc
    12481 tcgccgaagc ggcgcaggtg gcgcaggcgc ccgcgcagcg tggcggcggg catgatgctg
    12541 cgcgcgaaca cccacggctc gccggcgcac tcgagcagca cctcgcgcac cagtgccacc
    12601 tcgtgcgcgc cgaggcccag cgcgcgtcgc tcggaggcgc gcggacgctg ccatgcctgg
    12661 cgcagcacgc gcacgcgcag gcggttgccg caggctgcct ggagaatcgc ggtgagggag
    12721 ccttcgtcca gcagccagtg ccgcgtcgcc ggtggcaact gcgaggaatc gaaccgccgc
    12781 cagggcttcc aggcgggttc gccgggctgg aagcccgcgg acgctaacgc gtacaatgct
    12841 gcgctctccg tggtcaggac gccgctgatt ctagccgttc actcgccgcg gaagttcctc
    12901 gacgcggccc ggagcgtacc gggtgcgcga tgacaatgac agcagcgacg gcgcgatggc
    12961 ggcagggaga ttggcatggc tcggtgggaa tgtatcgttt gcggctgggt ctatgacgag
    13021 gccaagggct ggcccgacga tggcatcgcc gccggcacgc gctgggaaga cgtgccgaac
    13081 gactggctgt gcccggattg cggcgttggc aaggaagact tcgagctgat cgacgacacc
    13141 ccgctggtgg cggttccgca tcaccaggaa gccgatatcg tctcgctcac gcagccggtg
    13201 atcatcatcg gcaccgggct tgccggctac aacctggcgc gcgagttccg caagctcgac
    13261 agcgacacgc cgctgatcct ggtcacctcc gacgacggcc gcatgtattc caagccgatg
    13321 ctctcgaccg gcttcaccaa gaacaccacg cccgatgacc tcgcgaccat ggatgccggc
    13381 agcatggcga tcagcctgcg cgccagcgtc tggacgcaca cgcacgtgac cgccatcgac
    13441 accgcgaagc acgagatccg catcggcgag gccaccacgg tcggctacag caggctcgtg
    13501 atcgcctggg gtgccgacac catccgcccg ccgttgaagg gcgacggcct cgaccacgtc
    13561 tattcggtga acgatctgct cgattacgcg gattttcgcg ccgcgatcag gaacaacgac
    13621 gtgaagaagg tgctgatcat cggcgccggg ctgatcggct gcgaattcac caacgatctc
    13681 tgcaacggcg gtttcgcggt cgaggccgtc gacccgctcg gctggtgcct gcccacgctg
    13741 ctgcccgagc ccgcgggccg cgcggtgcag ggcgcgctcg aggcgctcgg cgcgaagttc
    13801 cacttcggcc cgctggtcag cgaggtgaac ctcgcgcccg gtggcaaggg catcgtggcg
    13861 acgctgaacg acgggcgtcg tatcgaggcc gacatggcgg tttcggcgat cggggtgcgc
    13921 ccgcgcctgg aactcgcgaa agcggccggc atcgcggtga accgcggcgt ggtcgcgaac
    13981 cgtctgctcg cgaccagcgc gcccgatgtc tacgtgctgg gcgactgcgc ggaggttgat
    14041 ggccacgtgc tgtattacgt cgcgccgctg atggcctgcg cgcgcgcgct cgccaagacg
    14101 cttgccggca cgccgaccga ggtcagctac ccggcgatgc cggtcaccat caagaccccg
    14161 gcctgcccgg tggtggtcgc gccgccgccg gtgggcgcgg ccggcgcgtg gtcgatcagc
    14221 ggcagcagcc cggatctcgt cgctgaattt cgcgatcccg cgggcaagct gctggggttc
    14281 gcgctgaccg gcgaaggcgg caagcagaag ctgcagctgc agaaggaact gccgccgatc
    14341 attgcctgag cgcaagctca ggccgcgagg tcgcgcgcaa tcgcctgcag ctccccggcc
    14401 gaaccgtgga tgcgctcgct cacggcgagt gcgtcggcgc ggctgatgcc gagctcggtg
    14461 aacatcgagt ccgcgatggg ctgcagcggc gcgctgccga tgtgctgttc gcgcagcagt
    14521 cgagtcgcca ggtgcagcac gcgtgccagc acctcgtgtt cgccggcctg ctccggcgcc
    14581 tgctggaagc gcagtgccgc gcagacttcc tccggcaggt gccagaagcg catcagccag
    14641 cccgccatct gttcacgcgt caccccgagc aggaagcgtt cgacatgcca gtgcccgagg
    14701 tgcgggttgg cctcggtgta gcggcagacc agttgcagct gcggcttgaa cagttccgcg
    14761 aggatcagga aaccgaagtt gtgcagcagc ccgccgagat aggcgtggcc cagcgcgggg
    14821 cgcaggctcg agggcatggc cttgcacagg ccctcgacca tcgcggcgcc gtgaacggcc
    14881 tgcagccagt agttgcggta accgtagcgc ccttccttcg gcaggcgcat ggtcttgccg
    14941 agcgccagcc ccagcgcgag gttcatcacc aggtcgaagc cgagcacgcg cacgatggcg
    15001 tcctggatgg acttcacctt gcccggcgcc gcgtagtacg gcgaggacgc ccagctgacc
    15061 acctgcgccg acagcgaggg gtctatttcg accacccgcg ccaggtcgct gacgctggca
    15121 taggggtcgg cgctgatgcg gatgatgcga tgggcgatct ccggcagtgg cgggaaatcg
    15181 agggtgtcct cgaggcggcg ctcgatgcgc cgcgcggtga agcgcgcgac cgcgttgccg
    15241 at
//
LOCUS       DAORUQ010000067        15053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3749_length_15053_cov_5.825977, whole genome shotgun sequence.
ACCESSION   DAORUQ010000067 DAORUQ010000000
VERSION     DAORUQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_15053_cov_5.825977"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PLW13_07730"
     CDS             complement(<1..1613)
                     /locus_tag="PLW13_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQY70301.1"
                     /translation="MSTTPRGFDASYDYIIIGAGSAGCALASRLSREASRHVLLLEAG
                     GPDRHPMIHVPLGFAFLMKDRSVNWCYQTEPEPELDYRKIAWPRGKVVGGTSAINGMV
                     YIRGQREDYEAWAAAGNPGWSYEEMLPWFLASEHNTRGADALHGSGGGLWVDEVGPPL
                     ECTDLFIQAGVSIGLPFNTDFNGPSQEGIGRYQVNVRNGVRQTAATSFLKPALKRANL
                     ELATHALAEKLLFDGERVTGVQYRRKDKVLRVAARRSVILCGGSINSPQLLELSGIGN
                     PAVLEPLGITTRHALPGVGENLQDHLTINVQQGLRNVRTFAEETTPLGLLRNALRWGI
                     RREGLLIHPACQAGAFLRSSPDVGRPDAQIHFTPAAGRLDARGNLVTVPGTTATVCNL
                     RPSSRGSVHARTPFPDYPPAIRANYMATEADRRVMIDAVRRVREIFASSVFDGYREAE
                     IRPGVNCSSDEQILAFIRREAESVYHPVGTCAMGAGEDAVVDHRLRVHGLEGLRIADA
                     SVIPDIISGNTNALCVAIGLKAAGMLIED"
     gene            1768..2721
                     /locus_tag="PLW13_07735"
     CDS             1768..2721
                     /locus_tag="PLW13_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HQY70302.1"
                     /translation="MAGASLLALLDDIATVLDDVAVMTKLAAKKTAGVLGDDLALNAQ
                     QVAGVRAERELPVVWAVAKGSARNKLILVPLALALSALAPWAVTPVLMIGGAYLCYEG
                     FEKLAHRFLHSAGEDAAAHEALEHVLLDPGTDMVAFETHKIGGAIRTDFILSAEIIVI
                     ALGTVQAASFAHRAAVVTVVAAAMTVGVYGFVAGIVKMDDAGLLLLRDESPGAGGRLK
                     RALGNALLWIAPRMMKTLSVLGTAAMFLVGGGILVHGMPPLAQWLHGAEHAAAGVPGV
                     GAVLAGVTPALLNAAAGIVAGAIVLAAVSAYGALTGRWRGR"
     gene            2765..3553
                     /locus_tag="PLW13_07740"
     CDS             2765..3553
                     /locus_tag="PLW13_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70303.1"
                     /translation="MDTTTLFSLAGRTALVTGGSRGIGRMIAAGFIAQGARVYISSRK
                     GAACEAVAGELGPNCIALAQDVSTVAGCRALAADYSAREPALDILVNNAGAAWGEPFE
                     SFPEAGWDKVMDLNLKSPFFLTQALHGALKAAATPARPAKVINIASVDGMRPNPWHTY
                     SYQASKAGLIHLTRRLAARLISDGIVVSAIAPGAFASDMNRAARDHGDLAARAIPSGR
                     IGRDEDMAGAAIYLASRAGDYVVGETLTVDGGLVNAALGESIDA"
     gene            complement(3609..4964)
                     /gene="nhaD"
                     /locus_tag="PLW13_07745"
     CDS             complement(3609..4964)
                     /gene="nhaD"
                     /locus_tag="PLW13_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQY70304.1"
                     /translation="MIAIIAIFCVGYLMIILEHNVNVDKAAPAVLTGVLCWAVYILAA
                     PGLIDVASLPQTFLDAHATKSTEEIIKAWVGEHQMLEGFAETASILFFLLGAMTIVEM
                     VDVYGGFTIITDRIRSTSMVKLLWIIGFVAFFMSAALDNLTTTIVMISLIRKLIDDKD
                     DRLFFAGIIVIAANAGGAWSPIGDVTTTMLWIGGQITTLRIMESIFLPSLVCLAIPLL
                     FLTFRLRGRQITSPVQVQNEAHHSVSKSQRNLIFLVGMGGLLFVPVFKTVTHLPPFMG
                     MLFSLGVLWVVSELVHKDRDAAMESGMHILTVVRRIDTPSVLFFLGILTAVSALQATG
                     LLAQAAGFLDRTVGNLDLIVFLIGLLSAVIDNVPLVAATMGMYDLAVHPTDSPLWEFL
                     AFAAGTGGSCLIIGSAAGVAAMGMERINFFWYVRNISGLALMGYVAGSAVIMLEKSVF
                     F"
     gene            5185..6051
                     /locus_tag="PLW13_07750"
     CDS             5185..6051
                     /locus_tag="PLW13_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HQY70305.1"
                     /translation="MAVERYCAPESVAEAVALLDPATGAAAFSGGTDLLVQMRAAVRS
                     PATLVDLKRIPGLAGVEFAPDAVRIGAATRCCALTANDELRALWPGLLEAAGLIGSTQ
                     VQGRASLGGNLCNASPAADSVPALVANRARALVAGPGGQRSVAVEDFVLAPGRTVLAS
                     GEFVHAIELPRPAPRTADAYLRFTPRTEMDIAVAGAGVSVTLDADGVCTGARIALGAV
                     APTVLLVPGAAAALIGSRCDEAALDACAGAARAAARPITDRRGSAELRRQVIGVLVRR
                     AVRVAVQRAGGT"
     gene            6154..6639
                     /locus_tag="PLW13_07755"
     CDS             6154..6639
                     /locus_tag="PLW13_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY70306.1"
                     /translation="MSIHVRTRINGQAQEFLCEPDQSALQVLRDTLGLTGSKEGCNSG
                     DCGACSVLLDGTLVCACLVLGAELEGRSLATIEGIAEGGRLHPVQQKLLEHNALQCGI
                     CIPGIVVAAKALLDHDPDPDETAVRYWLAGNLCRCTGYDKIIRAVLDAAATMRASGGA
                     P"
     gene            6636..8894
                     /locus_tag="PLW13_07760"
     CDS             6636..8894
                     /locus_tag="PLW13_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQY70307.1"
                     /translation="MNANEQGLRQIGTRPPRPDGVDKVTGRARYGADYTLPGMVWGRV
                     LRSPHAHARILGIDASAALALPGVLAVVTAEDFLAEPKGDVASGEAEIESGDLSRNVM
                     AREKALYHGHAVAAVAATTPRIAAAALAAIRVDYEALPAVLDIDAAMAPDAPLLHADM
                     FTRGVDPEPRAASNVAAITTLQRGDLDAGFAAAEVVVEGTWCSPMAHQGYIEPQACLA
                     CVGPGGEVELWCSTQGPFMVRSSVALLLGLDVADVRVIPTEIGGGFGGKTTVYIEPIA
                     VALSRKCGRPVKMQLSREEVFRATGPASASRVWVRIGARSDGTITAMDARLEYDAGAF
                     RGSPFIPGAMSIFACYDVPNQRAVALDVVTNKPKVAAYRAPGAPQAILASECALNELA
                     ARLGIDPIELRLRNAVREGSRTIYGAAFETIGLVECLEQARRHPHYAAALQANQGRGV
                     AAGFWFNAGLQSSAAVHLTENGKLLLEEGSPDIGGSRAAMALIAAETLGMPYASVKPA
                     VVDTSGIGYTDLTGGSRTTYATGYAVIEACRDLIAKLSARAALEWGVDAARVSWRDGA
                     ACHPDAPGTTLDIVALARLAARTGGPLSGYGSVNLKHVAPAFAVDIADVEVDPETGAS
                     RVVRFTAIQDAGRAIHPDFVEGQMQGGAAQGIGWALNEEYRFDARGVLENPGFLDYRM
                     PVASDLPMIDTVIVEVPHPGHPLGVRGVGEAPICAPMAAVTSALNQAAGTSIRALPLT
                     PSRVLAAIGARG"
     gene            8896..9141
                     /locus_tag="PLW13_07765"
     CDS             8896..9141
                     /locus_tag="PLW13_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70308.1"
                     /translation="MARIVFLAAQRRFTGGCHEMHATVRDFRGVVAAVRERFPEFPES
                     ELLGCSVAIDGEIVNRPWLEPIGADSELIFVARIGAG"
     gene            9145..10590
                     /gene="mgtE"
                     /locus_tag="PLW13_07770"
     CDS             9145..10590
                     /gene="mgtE"
                     /locus_tag="PLW13_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009399023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQY70309.1"
                     /translation="MDQQQREQYHRSLEATLVETLELLEKHRLERELSLRQASGQREL
                     VDALLARQHKGEFQRRLARMHPADIAFVLERLPTDDRLELWALVDAERRGGVLLELAD
                     GVRDSLIAAMPQREIVDAAEHLETDEIAYLVPSLPQETVAALLSSLDQRERHKVREML
                     AFPEGTVGALMDIEMVTVRDDVVIEVVLRYLRRHEELPDPFDQVFVVDRDGVLVGILR
                     LKDLLTHDPDRPVAEVMLREPLTFSTHDPASDAVGVFERYDLVSVPVVNLHQRLTGVL
                     HFAAVMDFISEQAESQQLKQVGLTEDEDLFAPVMDSARNRWPWLGLNLLTALVASRVI
                     GAFEATIQQQVALAALMPVVASIGGNTGNQTVALVIRGMALEQIVASSLWVVVRKELA
                     IALVNGLLWGSVVALAALLFYRQPALSLVMGSAVAVNLLIASLAGVFIPLALKQTGRD
                     PVLGSSVLLTFVTDAMGFLVFLGLAAWLIVG"
     gene            10628..11095
                     /locus_tag="PLW13_07775"
     CDS             10628..11095
                     /locus_tag="PLW13_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HQY70310.1"
                     /translation="MIVRTLFALLLCFACAACTPSAPRDEARGAEAGDALAIGRGARC
                     FRVLSVDNWRVIDERQLVVYGRGRGETWHLQLFAPCQGLRFTETLGFRASGTDWICGD
                     PGDVVVWRENRCPISAVSRVSPAQLKMLFDKDVREDIGRMPAGAPATGDRNEE"
     gene            11098..11613
                     /locus_tag="PLW13_07780"
     CDS             11098..11613
                     /locus_tag="PLW13_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY70311.1"
                     /translation="MTTEFSLNGERVTVEGDAQKPLLWVLREDLALTGAKFGCGKGLC
                     GACTVQVDGVAVRSCSFPLALAAGRSVTTIEGLGRDGLHAVQQAWVEMNTPQCGYCQP
                     GQIMSAAALLAANAAPSDADIDAAMAGNLCRCGTYPRIRKAIHHAAALLAAEQAESAQ
                     EIVPPAEELEP"
     gene            11610..13796
                     /locus_tag="PLW13_07785"
     CDS             11610..13796
                     /locus_tag="PLW13_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY70312.1"
                     /translation="MMNMNRRAFLRNVSIAGGGVMLGVTLGGCAGDAPPWPNRQTGVL
                     QPDAFLQVRPDGSVVLAIHKAEMGQGVATGLATLVAEELEIDPLRLQLEFAEPHPDYN
                     DKEYRSMITGGSASMRNNFDGMREAGAALREMLRQGAAASWGVALADCIARDGAVSLR
                     DGSRSASYGELAAAAAKLPVPTGVALKPPAEWRRIGKHDARVDAVAKVDGSAPFALDV
                     RLPGMLTAVLVRCPHFGGTLKGFTPDKAQAVAGVRQVLELNGAVAVIAQGYWEARSAA
                     ALLEIEWDKGPLAGLDSEAITAGQRRVLAEESGRVVREDGEEPPAGTVARSLSAEYRV
                     PYVAHAALEPMNAVASVTASGAEVWAGNQAPDVLQGLVARLLEIEPQNVRVHSTYIGG
                     AFGRRTYMEFALEAVALSRAVGAPVKVIWSREDDTRHDHYRPAAMASMRADFGDDGKL
                     LGWDARVVAPSVMNTMVGVMLPVMLPRWAPLGVGSVIEPLVARYDPTLTEGVDSIPYT
                     MPRVRVEGLVWDPGIPVGVWRSVGHSQNAFFSEGFVDEVAHALGEDPVRFRLSRLPAD
                     SRVRGALELLVAKGGWGSAPAGIYQGIAVHESFGTVVAEQVDIALENGAPVVRRVVCA
                     VDCGVVINPDVVRMQLESAVVFALSAALHGEITIRDGAVVQGNFDDFPLVRIDASPEV
                     EVHFVATDRPPSGIGEPGVPPLAPALANAIFAATGKRLRELPLRVS"
     gene            13845..14846
                     /locus_tag="PLW13_07790"
     CDS             13845..14846
                     /locus_tag="PLW13_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY70313.1"
                     /translation="MALFGLRYDLRSAPGSAVAKPALYRAALEQCAWADRLGFAMVTL
                     SEHHGSPDGYLPSPMVMAAAIAARTERLRIMISTLVATLYDPVRLAEDFAVLDNLSNG
                     RILPVLSAGYVASEFESFGKRLGERRKTMDGIAGFLRQAWTGEPFAYEGRTVCVTPRP
                     AQARPPIWMGGSSRAAAARAARDADNFVPSSPEFFDMYREELLKLGKPDPGPRPASLG
                     RFLYLAEDPDAAWEEIAPYLMHENNAYAAWADAQGVATSYRHYASAQELRDTGTYPVM
                     TPRQLLEHIEAAGPYDMVLFHPLAGGMEPGLAWRSLRLFEQEVYPQTGLDSSRSEGK"
     gene            14843..>15053
                     /locus_tag="PLW13_07795"
     CDS             14843..>15053
                     /locus_tag="PLW13_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70314.1"
                     /translation="MNKRLQGQVAVITGAGQGVGRGVARRMAIEGATVVVGEINTENG
                     RRVAGECEELGGRGIFVHTDVTQRDS"
BASE COUNT         2192 a         5036 c         5578 g         2247 t
ORIGIN      
        1 tcgtcctcga tcagcatccc ggcggccttg aggccgatgg cgacgcagag cgcgttggtg
       61 ttgccgctga tgatgtcggg aatcaccgac gcatcggcga tgcgaaggcc ctcgaggccg
      121 tgcacacgca ggcgatggtc gaccaccgcg tcctcgcccg cgcccatcgc gcaggtgccg
      181 accgggtggt agaccgactc ggcctcgcgg cggatgaacg cgaggatctg ctcgtcgctg
      241 ctgcagttca cgccgggccg gatctccgct tcacggtagc cgtcgaacac gctggaggcg
      301 aagatctcgc gcacgcgcct gaccgcgtcg atcatcacgc gccggtccgc ctcggtggcc
      361 atgtagttgg cgcggatcgc cggcggatag tcggggaagg gcgtgcgcgc gtgcacgctg
      421 ccgcggctgc tcgggcgcag gttgcatacg gtcgcggtgg tgccgggcac ggtgacgaga
      481 ttgccgcgcg cgtcgaggcg cccggcggca ggcgtgaagt ggatctgcgc gtcggggcgc
      541 ccgacgtcgg gcgagctgcg caggaaggcg ccggcctggc aggcggggtg gatcagcagc
      601 ccctcgcgcc tgatgcccca gcgcagcgcg ttgcgcagca ggccgagcgg cgtggtctcc
      661 tcggcgaaag tcctcacgtt gcgcagcccc tgctgcacgt tgatcgtcag gtggtcctgc
      721 aggttctcgc ccacgccggg cagcgcgtgc cgggtggtga tacccagcgg ctcgagcacg
      781 gcgggattgc cgatgccgga gagctccagc agctgcgggc tgttgatcga gccgccgcag
      841 aggatcaccg agcggcgcgc agccacgcgc agcaccttgt ccttgcggcg gtactgcacg
      901 ccggtgacgc gctcgccatc gaacagcagc ttctcggcaa gggcatgcgt cgccagctcg
      961 aggttggcgc gcttgagcgc gggcttgagg aaactggtcg ccgcggtctg gcgcacgccg
     1021 ttgcgcacgt tgacctggta gcggccgatg ccttcctggc tcgggccgtt gaagtcggtg
     1081 ttgaagggca ggccgatgct cacgcccgcc tggatgaaca ggtcggtgca ctcgagcggg
     1141 gggccgacct cgtcgaccca cagcccgccg ccgctgccgt gcagcgcgtc ggcgccgcgc
     1201 gtattgtgct cgctggcgag gaaccagggc agcatctcct cgtaggacca gccgggattg
     1261 ccggccgcgg cccaggcttc gtagtcctcg cgctggccgc ggatgtagac catgccgttg
     1321 atggcgctgg tgccgcccac caccttgccg cgcggccagg cgatcttgcg gtagtcgagc
     1381 tcgggctcgg gctcggtctg gtagcaccag ttgacgctcc tgtccttcat caggaaggcg
     1441 aagccgagcg gcacgtggat catcgggtgg cggtccggcc cgccggcctc gagcaacagc
     1501 acgtgccggc tcgcttcgcg gctgaggcgc gaggcgagcg cgcagccggc cgagccggcg
     1561 ccgatgatga tgtagtcata gctggcgtcg aagccgcgcg gggtggtgct caagcgggtg
     1621 cgctccggta ctgcgtgagg cggccattgt cggcgatacc ggcgcagcgc tccacgcccg
     1681 cctgattacg ctttggtaag cggcggctgg cgccgcccca cgcgattgtg gtcagatgcg
     1741 cggctcacaa ggacggggat atgcacgatg gccggggcca gcctgctcgc gctgctcgac
     1801 gacatcgcaa cggtgctcga cgacgtcgcg gtgatgacca agctcgcggc gaagaagacc
     1861 gccggggtgc tcggcgacga tctcgcgctg aacgcgcagc aggtcgccgg cgtgcgcgcc
     1921 gagcgcgagc tgccggtggt ctgggcagtc gcgaagggtt cggcgcgcaa caagctgatc
     1981 ctggtgccgc tggcgctcgc gctcagcgcg ctggcgccgt gggccgtgac gccggtgctg
     2041 atgatcggcg gcgcctacct gtgctacgag ggcttcgaga agctcgcgca ccgcttcctg
     2101 cacagcgccg gcgaggacgc cgcggcgcac gaggcgctgg agcatgtgct gctcgacccc
     2161 ggcaccgata tggtcgcctt cgagacgcac aagataggcg gcgccatccg caccgatttc
     2221 atcctctcgg ccgagatcat cgtgatcgcg ctgggcacgg tgcaggcggc gtcctttgcc
     2281 caccgggccg cggtggtcac ggtggtcgcg gcggcgatga ccgtgggcgt ttacggcttc
     2341 gtggccggca tcgtgaagat ggacgacgcc ggcctgctgc tgttgcgcga cgaatcgccg
     2401 ggcgcggggg gcaggctgaa acgcgccctc ggcaacgcgc tgctgtggat cgcgccgcgg
     2461 atgatgaaga cgctgtcggt gctcggcacg gcggcgatgt tcctggtcgg cggtggcatc
     2521 ctggtgcatg gcatgccgcc gcttgcgcaa tggctgcacg gcgccgaaca cgccgccgcc
     2581 ggcgtgcccg gcgtgggcgc ggtgctggcc ggcgtcacgc cggcgctgct caacgccgcc
     2641 gccggcatcg tggcgggggc gatcgtgctg gccgcggtca gcgcttacgg cgcattgaca
     2701 ggacgatggc gcgggcgctg aggcagcgcg ccgggcaatt gcaggcaaga cggaggtact
     2761 gcgagtggat acgacgaccc tgttctcgct cgccgggcgc accgcgctgg tcaccggcgg
     2821 ctcgcgcggc atcggccgca tgatcgcggc gggtttcatc gcccagggcg cgcgcgtcta
     2881 catatcttcg cgcaagggcg cggcgtgcga ggcggtcgcc ggggaactgg gcccgaactg
     2941 catcgcgctg gcgcaggacg tctccacggt cgcgggttgc cgcgcgctgg cggcggacta
     3001 cagcgcgcgc gagcccgcgc tcgacatcct ggtcaacaac gccggcgcgg cctggggcga
     3061 gcccttcgaa agctttcccg aagcgggctg ggacaaggtc atggacctga acctcaagtc
     3121 gccgttcttt ctcacccagg cgctgcacgg cgcgctgaag gcggcggcca cccccgcgcg
     3181 tccggccaag gtgatcaaca tcgcctcggt cgacggcatg cgtcccaatc cctggcacac
     3241 ctacagctac caggcctcga aagccgggct gatccacctg acgcggcgac tggcggcgcg
     3301 gctgatcagc gatggcatcg tggtcagcgc gatcgcgccg ggggcgtttg cctccgatat
     3361 gaatcgcgcg gcgcgcgatc atggcgatct cgccgcacgg gcgataccgt ccgggcgcat
     3421 cggcagggac gaggacatgg cgggcgcggc gatctatctg gcgagccgag ccggcgatta
     3481 cgtggtgggg gaaacgctca ccgtcgacgg cgggctggtg aatgccgcac tgggcgagtc
     3541 gatcgacgcg tagggggatg caggcagcgg cggccggatc ctgccgccgc tgcctgcgat
     3601 gcgctcgatc agaagaagac cgatttttcc agcatgatca ccgcagagcc ggccacgtag
     3661 cccatcaacg ccaggccgct gatattgcgc acgtaccaga agaagttgat gcgctccatg
     3721 cccatcgcgg ccacgccggc cgccgagccg atgatcaggc agctgccgcc ggtgcccgcg
     3781 gcgaaggcca ggaactccca cagcggggaa tcggtcggat gcaccgccag gtcgtacatg
     3841 cccatggtcg cggcgaccag cggcacgttg tcgataacgg ccgagagcag gccgatcagg
     3901 aacacgatca ggtcgaggtt gcccacggta cggtccagga agccggccgc ctgcgcgagc
     3961 agccccgtcg cctgcagcgc ggagacggcc gtcaggatgc cgaggaagaa cagcacgctg
     4021 ggagtgtcga tgcggcgcac caccgtgagg atgtgcatgc ccgattccat cgcggcgtcg
     4081 cggtccttgt gcaccagctc tgacaccacc cacagcacgc cgaggctgaa cagcatgccc
     4141 atgaagggcg gcaggtgcgt cacggtcttg aacaccggca cgaacagcag gccgcccatg
     4201 cccaccagga agatcaggtt gcgctgcgat ttgctgaccg agtgatgggc ctcgttctga
     4261 acctgcacgg ggctggtgat ctgccgcccg cgcaggcgga aggtgaggaa cagcagcggg
     4321 atcgcgaggc acaccaggct cggcaggaag atcgattcca tgatgcgcag cgtggtgatc
     4381 tgcccgccga tccacagcat cgtggtcgtg acgtcgccga tcggtgacca ggcgccgccg
     4441 gcgttggccg cgatcacgat gatgccggcg aagaacagcc ggtcgtcctt gtcgtcgatc
     4501 agcttgcgga tcagcgagat catcacgatc gtggtggtca ggttgtcgag tgccgccgac
     4561 atgaagaagg cgacgaagcc gatgatccac agcagcttga ccatgctggt cgagcggatg
     4621 cggtcggtga tgatcgtgaa gccgccgtag acgtcgacca tctcgacgat ggtcatcgcg
     4681 ccgagcagga agaacaggat gctcgcggtc tcggcgaagc cctcgagcat ctggtgctcg
     4741 ccgacccagg ccttgatgat ctcctcggtg gacttggtcg cgtgcgcgtc caggaaggtc
     4801 tgcggcaggc tggccacatc gatcagcccc ggggcggcga ggatgtagac cgcccagcac
     4861 agcacgccgg tcagcaccgc gggcgctgcc ttgtcgacgt tgacgttgtg ctcgaggatg
     4921 atcatcaggt agccgacaca gaagatggcg atgatggcga tcagcggtaa tggcatgtgt
     4981 ctcttcccgg ctactggggg tgcgtggatc caagcgcgct aatttaagcc gagaggcgtg
     5041 ccggcgacca tgcgcgccag cgtgcaaatt gccgaagcgc gaggccgtgc tgcccgggga
     5101 cgcgcgaacg ggcggttttg tttgtccggg cgagcggcta tgctccccgg cttgcaacga
     5161 gcacccaggc aggggaggtc ccacgtggcg gtagaacgat actgtgcccc ggaatcggtg
     5221 gcggaggcgg tggcgctgct cgacccggcg acaggcgcgg cggcattttc gggcggcacc
     5281 gacctgctgg tgcagatgcg cgcggcggtg cgctcgccgg cgacgctggt cgatctcaag
     5341 cgcattcccg gactggccgg cgtggagttc gcgcccgatg ccgtgcgcat cggcgccgcg
     5401 acgcgctgct gcgcactgac cgcgaacgac gaactgcggg cactgtggcc gggcctgctg
     5461 gaggccgccg gcctgatcgg ctccacgcag gtgcagggtc gcgccagcct cggcggcaac
     5521 ctgtgcaacg cctcgcccgc cgcggactcg gttcccgccc tggtggccaa tcgcgcgcgg
     5581 gccctggtcg cgggacccgg cggccagcgc agcgttgccg tcgaggactt cgtgctcgcg
     5641 ccggggcgca ccgtgctcgc cagcggcgag tttgttcacg ccatcgagtt gccgcgcccg
     5701 gcgccgcgca ccgcggatgc ctacctgcgc ttcacgccgc gcaccgagat ggatatcgcg
     5761 gtggccggcg cgggagtatc cgtgacgctg gacgccgacg gtgtatgcac cggtgcgcgc
     5821 atcgcgctgg gcgcggtcgc gcctacggtg ctgctggtgc ccggggccgc cgcggcgctg
     5881 atcggctcgc gctgcgacga ggcggcgctg gatgcctgcg ccggcgccgc gcgcgcggcg
     5941 gcacggccca tcaccgatcg gcgcggcagc gcggagctgc gccgccaggt catcggcgtg
     6001 ctggtgcgtc gggccgtgcg ggtggccgtg cagcgcgcgg gtggcaccta gatcgcggtc
     6061 gggccgcggg aatcaggcgg ctgcgagcgg gccgcggggc agcgccgcga ctgcgtgcgc
     6121 cgtcaattgc gaaattcgtc gggggtggag cagatgtcga tacacgtacg aacgcggatc
     6181 aacggacagg cgcaggaatt tctctgtgaa ccggaccaga gcgcgctgca ggtgctgcgc
     6241 gacacgctcg ggctgaccgg gtccaaggag ggctgcaact ccggggactg cggcgcctgc
     6301 tcggtgctgc tcgacggcac gctggtgtgc gcgtgcctgg tgctcggcgc cgagctcgag
     6361 ggacgcagcc tcgccacgat cgagggcatc gccgaaggcg gcaggctgca cccggtgcag
     6421 cagaagctgc tcgagcacaa cgcgctgcag tgcggcatct gcatccccgg catcgtggtc
     6481 gcggcgaagg cgctgctgga ccacgatccc gacccggacg agaccgcggt gcgctactgg
     6541 ctggcgggca atctctgccg ctgcaccggc tatgacaaga tcatccgcgc ggtgctcgat
     6601 gcggccgcga cgatgcgcgc gagcggaggt gcgccatgaa tgcgaatgag caggggttgc
     6661 gccagatcgg cacgcgcccg ccgcgtcccg acggcgtcga caaggtcacg gggcgcgcgc
     6721 gctacggcgc ggactacacg ctgccgggca tggtctgggg ccgcgtgctg cgcagcccgc
     6781 acgcgcacgc gcgcatcctc ggcatcgacg cgagcgccgc gctcgcgctg cccggcgtgc
     6841 tggccgtggt gacggcggaa gactttctcg ctgaacccaa aggtgatgtg gccagcggcg
     6901 aggccgagat cgagagcggc gatctcagcc gcaacgtgat ggcgcgcgag aaggcgctgt
     6961 accacggcca cgcggtggcg gcagtcgcgg cgaccacgcc gcgcatcgcg gcggcggcgc
     7021 tggccgcgat ccgcgtcgac tacgaagcgc tccccgcggt gctggacatc gacgccgcga
     7081 tggcacccga tgcgccgctg ctgcacgcgg acatgttcac gcgcggggtc gaccccgaac
     7141 cgcgtgccgc ctccaacgtg gctgcgatca cgaccctgca gcgcggcgac ctcgatgcgg
     7201 gcttcgctgc ggccgaggtc gtcgtggaag gcacgtggtg ctccccgatg gcacaccagg
     7261 ggtatatcga gccgcaggca tgcctggcct gcgtggggcc gggcggggaa gtcgagctct
     7321 ggtgcagcac gcaggggccg ttcatggtgc gctccagcgt cgcgctgctg ctggggctcg
     7381 atgtcgccga cgtgcgcgtc attcccaccg agatcggcgg cggcttcggt ggcaagacca
     7441 cggtctacat cgaaccgatc gccgtggcgc tgtcgcgcaa gtgcggccgg ccggtgaaga
     7501 tgcagctctc gcgcgaggaa gtgttccgcg ccaccggccc cgcctcggcc tcgcgcgtgt
     7561 gggtcaggat cggcgcgcgc tccgacggca cgatcaccgc gatggacgcg cgactcgaat
     7621 acgatgccgg cgccttccgc ggctcgccgt tcataccggg cgcaatgtcg atctttgcct
     7681 gctatgacgt gcccaaccag cgcgcggttg ctctcgacgt ggtcaccaac aagccgaaag
     7741 tcgccgccta ccgcgccccc ggcgcgccgc aggcgatcct ggcgagcgaa tgcgcgctca
     7801 acgagctcgc cgcgcggctc ggcatcgacc cgatcgagct gcgcctgcgc aacgcggtgc
     7861 gcgagggctc gcgcacgatc tacggcgcgg ccttcgagac catcggtctc gtcgaatgcc
     7921 tggagcaggc gcgccggcat ccgcattacg cggcggcgct gcaggcgaac caggggcgcg
     7981 gcgtggccgc cggcttctgg ttcaacgcgg gcttgcagtc gagcgccgcg gtgcacctga
     8041 ccgaaaacgg caagctgctg ctcgaggaag gcagccccga catcggtggc tcgcgcgccg
     8101 cgatggcgct gatcgcggcc gagacgctgg gcatgcccta tgcgagcgtc aagccggccg
     8161 tggtcgatac ctcgggcatc ggctacaccg acctgaccgg gggcagccgc accacctatg
     8221 ccaccggcta cgccgtgatc gaggcctgtc gcgacctcat cgcgaagctg agcgcgcgcg
     8281 cggcactcga gtggggcgtc gatgccgcgc gcgtcagctg gcgcgacggc gcggcctgcc
     8341 acccggacgc gccggggact acgctcgaca tcgtggcgct cgcacgcctg gcggcgcgta
     8401 ccggcggacc gctgagcggc tatggcagcg tgaacctgaa gcacgtcgcg ccggcattcg
     8461 cggtcgatat cgccgatgtc gaggtcgatc ccgagaccgg cgccagtcgc gtcgtgcgct
     8521 tcaccgcgat ccaggacgcg ggccgtgcga tccaccccga ttttgtcgag ggacagatgc
     8581 agggcggcgc ggcgcagggg atcggctggg cgctgaacga ggaataccgc ttcgatgcgc
     8641 ggggcgtgct ggagaatccc ggcttcctcg attaccgcat gccggtggcc tccgacctgc
     8701 cgatgatcga caccgtgatc gtcgaggtgc cgcacccagg ccacccgctc ggcgtgcgcg
     8761 gcgtggggga ggcccccatc tgtgcgccga tggcggcggt caccagcgcg ctgaaccagg
     8821 ccgccggcac gagcatccgc gcgttgccgc tgacgccctc gcgcgtgctc gcggcgatcg
     8881 gcgcgcgcgg ctgaggtggc caggatcgtg ttcctggcgg cgcagcggcg cttcacgggc
     8941 ggctgccacg agatgcacgc cacggtgcgc gacttccgtg gcgtggtggc cgcggtgcgc
     9001 gagcgattcc cggaatttcc cgaatccgag ctgctcggct gcagtgtcgc gatcgatggc
     9061 gagatcgtga accggccctg gctggagccc atcggcgccg acagcgaact catcttcgtc
     9121 gcgcgcatcg gcgcgggcta ggccatggac cagcaacagc gcgagcagta ccaccgcagc
     9181 ctcgaagcca ccctggtcga gacgctcgag ctgctggaga agcaccggct cgagcgcgaa
     9241 ctgagcctgc gccaggcctc gggccagcgc gagctggtgg acgcgctgct ggcgcgccag
     9301 cacaagggcg agttccagcg ccgcctggcg cgcatgcacc cggccgacat cgccttcgtg
     9361 ctggagcgcc tgcccaccga cgatcgcctg gaactgtggg cgctggtgga tgccgagcgg
     9421 cgcggcggcg tgctgctcga gctcgccgac ggcgtgcgcg acagcctgat cgcggccatg
     9481 ccccagcgcg agatcgtcga tgccgcggag cacctcgaga ccgacgagat cgcctacctg
     9541 gtgcccagcc tgccgcagga aacggtggcg gcgctgttgt cctcgctcga ccagcgcgag
     9601 cgccacaagg tgcgcgagat gctcgccttc ccggaaggga cggtgggcgc gctgatggac
     9661 atcgagatgg tcacggtgcg cgacgacgtc gtgatcgaag tggtgctgcg ctacctgcgc
     9721 cgccacgagg agttgcccga tcccttcgac caggtcttcg tggtggaccg cgacggggtg
     9781 ctggtgggca tcctgcgcct gaaggacctg ctgacccacg atcccgaccg cccggtcgcc
     9841 gaggtgatgc tgcgcgagcc gctcacgttc agcacgcacg acccggccag cgacgcggtc
     9901 ggcgtgttcg agcgttacga cctggtgtcg gtgccggtgg tgaacctgca ccagcgcctc
     9961 acgggcgtgc tgcacttcgc cgccgtgatg gacttcatca gtgagcaggc cgagagccag
    10021 cagctcaagc aggtcggcct caccgaggac gaggacctgt tcgcgccggt gatggacagc
    10081 gcgcgcaacc gctggccgtg gctgggcctg aacctgttga cggcgctcgt cgcctcgcgc
    10141 gtcatcggcg ccttcgaggc cacgatccag cagcaggtgg cgctggccgc gctgatgccg
    10201 gtcgtcgcca gcatcggcgg caacaccggc aaccagacgg tcgcactggt gatccgcggg
    10261 atggcgctcg agcagatcgt cgcgagcagc ctgtgggtgg tggtgcgcaa ggagctcgcg
    10321 atcgcgctcg tcaacgggct gctgtggggc agcgtggtcg cgctggcggc gctgctgttc
    10381 taccgccagc cggcgctgtc actggtcatg gggagcgcgg tggccgtcaa cctgctgatc
    10441 gcctcgctcg ccggcgtttt catcccgctg gcgctgaagc agaccgggcg cgatccggtg
    10501 ctgggctcct cggtgctgct cacattcgtc accgatgcga tgggcttcct ggtgttcctc
    10561 gggctcgcgg cgtggttgat cgtcggctga atgccgcgcg cgccgggtgc aacatgagga
    10621 tatggtcatg atcgtgagga ccctgttcgc gttgctgcta tgcttcgcct gcgccgcgtg
    10681 cacgccgtcg gcgccgcgtg atgaagcccg cggcgccgag gccggcgacg cgctcgccat
    10741 cgggcgtggc gcacgctgct ttcgcgtctt gagcgtcgac aactggcgcg tgatcgacga
    10801 gcgccagctc gtggtatacg ggcgggggcg cggcgagacc tggcacctgc agctgttcgc
    10861 gccctgccag ggactgcgct tcaccgagac gctgggcttt cgcgccagcg gcaccgactg
    10921 gatctgcggc gaccccggcg atgtcgtggt ctggcgtgaa aaccgctgcc cgatcagcgc
    10981 cgtgagccgc gtttcgcccg cgcagctgaa gatgctgttc gacaaggacg tgcgcgagga
    11041 catcggcagg atgcctgcgg gagcgcccgc gaccggggac cgtaacgagg aatgagcatg
    11101 acgacggaat tcagcctcaa cggcgagcgc gtcaccgtgg agggtgatgc gcagaaaccg
    11161 ctgctgtggg tactgcgcga ggatctcgcg ctcacgggcg ccaagttcgg ctgcggcaag
    11221 gggctgtgcg gcgcctgcac cgtgcaggtc gatggtgtcg cggtgcgttc ctgctcgttt
    11281 ccgctcgccc tcgccgcggg caggtcggtg accaccatcg agggcctggg acgcgacggg
    11341 ctgcacgcgg tgcagcaggc ctgggtcgag atgaacacgc cccagtgcgg ctactgccag
    11401 cccgggcaga tcatgtcggc cgcggcgctg ctcgcggcca acgccgcgcc gagcgacgcc
    11461 gacatcgatg ccgcgatggc gggcaacctc tgtcgctgcg gcacctatcc ccgcattcgc
    11521 aaggcgatcc accacgccgc ggcgctgctc gcggccgagc aggcggaatc ggcgcaggaa
    11581 atcgtgccgc ccgccgagga gctcgagcca tgatgaacat gaaccggcgc gcattcctgc
    11641 gcaacgtctc gatcgccggt ggtggcgtca tgctcggcgt cacgctgggc ggctgcgccg
    11701 gggacgcccc cccgtggccg aaccggcaga cgggcgtgct gcaacccgat gccttcctgc
    11761 aggtgcgccc ggacggcagc gtggtgctgg cgatccacaa ggccgagatg gggcagggcg
    11821 tggcgaccgg gctcgcgacc ctggtggccg aggaactcga gatcgaccct ctgcgcctgc
    11881 agctggaatt cgccgagccg cacccggact acaacgacaa ggaataccgc tcgatgatca
    11941 cgggcggcag cgcctcgatg cgcaacaact tcgacggcat gcgagaggcg ggcgcggcgc
    12001 tgcgcgagat gctgcgccag ggcgccgccg cgagctgggg cgtggcgctc gccgactgca
    12061 tcgcgcgcga cggcgccgtc agcctgcgcg acggctcgcg cagcgcgagc tacggcgagc
    12121 tcgccgcggc cgcggccaag ctgccggtac ccaccggggt cgcgctcaag ccgcccgccg
    12181 agtggcggcg tatcggcaag cacgatgcgc gcgtggacgc cgtggccaag gtcgatggca
    12241 gcgcgccgtt cgcgctcgac gtccgcctgc ccgggatgct cacggcggtg ctggtgcgct
    12301 gcccgcactt cggcggcacg ctgaagggat tcacgcccga caaggcgcag gccgtcgccg
    12361 gggtgcgcca ggtactggaa ttgaacggcg cggtggccgt gatcgcgcaa gggtactggg
    12421 aggcgcgcag cgccgccgcg ctgctcgaga tcgaatggga caaggggccg ctcgccgggc
    12481 tggattccga ggcgatcacg gcgggccagc gccgcgtgct ggccgaggag agcgggcgcg
    12541 tggtgcgcga ggacggcgaa gagccgccgg cggggacggt ggcgcggtcg ctgagcgccg
    12601 agtaccgcgt gccgtatgtc gcgcacgcgg cgctcgaacc gatgaacgcc gtggcttccg
    12661 tgacggcctc cggcgccgag gtctgggccg gcaaccaggc ccccgacgtg ctgcagggcc
    12721 tggtggcgcg gctgctcgag atcgagccgc agaacgtcag ggtgcattcg acgtacatcg
    12781 gcggcgcctt cgggcggcgc acctacatgg agtttgcgct cgaggcggtc gcgctgtcgc
    12841 gcgcggtcgg cgccccggtc aaggtgatct ggtcgcgcga ggacgatacc cggcacgatc
    12901 actaccggcc cgcagcgatg gcctcgatgc gcgccgattt cggtgacgat ggcaagttgc
    12961 tcggctggga cgcgcgggtc gtcgcaccct cggtgatgaa cacgatggtc ggcgtcatgt
    13021 tgccggtgat gctgccgcgc tgggcgccgc tcggcgtggg ctccgtcatc gagccgctgg
    13081 tcgcgaggta cgaccccact ctcacggagg gcgtcgactc gatcccgtac acgatgcccc
    13141 gggtccgggt cgagggcctg gtgtgggatc cgggcatacc ggtcggggtg tggcgctcgg
    13201 tggggcattc gcagaatgcc tttttcagcg agggcttcgt cgacgaggtc gcgcacgcgc
    13261 tgggcgagga tccggtgcgc tttcggctct cgcgattgcc cgccgattcg cgcgtgcgcg
    13321 gggcgctgga gttgctggtc gccaagggcg gctggggcag cgcgcccgcg ggcatctacc
    13381 agggcattgc ggtgcacgag agcttcggca cggtggtggc cgagcaggtg gacatcgcac
    13441 tggaaaatgg cgcgcccgtc gtgcgccgcg tggtctgcgc ggtggattgc ggcgtggtga
    13501 tcaacccgga cgtggtgcgc atgcagctcg agagcgcggt ggtgttcgcg ctctcggccg
    13561 cgctgcacgg cgagatcacc atccgcgacg gcgccgtggt gcagggcaac ttcgacgatt
    13621 ttccgctggt gcgcatcgac gccagtcccg aggtcgaggt gcatttcgtg gccactgatc
    13681 gtccgcccag cggtatcggc gagcccgggg tgccaccgct cgcgccggcg ctcgccaacg
    13741 cgatcttcgc ggccacgggc aagcggctgc gtgagttgcc gctgcgggtt tcctgagatg
    13801 gcctgcgcat gccggcaaga atgccgaccc acggggggtg gctgatggcc ttgttcgggc
    13861 tgcgctacga cctgcgctcg gcgccgggca gcgccgtcgc gaagccggcg ctgtatcgtg
    13921 ccgcgctcga gcagtgcgcg tgggccgacc ggctcggctt cgcgatggtc acgctgtcgg
    13981 agcaccacgg atcgcccgat ggctacctgc cgtcgccgat ggtgatggcc gcggccatcg
    14041 cggcgcgcac cgagcgcctg cgcatcatga tctccacgct ggtcgcgacg ctctacgatc
    14101 ccgtgcgcct cgcggaggat ttcgcggtgc tcgacaacct gagcaacggg cgcatcctgc
    14161 cggtgctgag cgccggttac gtggcctccg aattcgagag cttcggcaag cgcctgggcg
    14221 agcgcaggaa gacgatggac ggcatcgccg gcttcctgcg ccaggcctgg accggcgagc
    14281 ccttcgccta cgagggacgc acggtgtgcg tgacgccacg cccggcgcag gctcgcccgc
    14341 cgatctggat gggcggcagc tcgcgggccg ccgccgcgcg cgcggcgcgc gatgccgaca
    14401 acttcgtgcc ctccagcccc gagttcttcg atatgtaccg cgaggaactc ctgaaactcg
    14461 gcaagcccga tcccgggccc cgtccggcca gcctcgggcg cttcctgtac ctggccgagg
    14521 atcccgatgc ggcctgggag gagatcgccc cgtacctgat gcacgagaac aacgcctacg
    14581 ccgcctgggc cgatgcacag ggcgtggcaa cctcctaccg gcactatgcg agcgcgcagg
    14641 aactgcgcga caccggcact tatccggtga tgacgccgcg ccagctgctc gagcacatcg
    14701 aggccgcggg gccctacgat atggtgctgt tccacccgct ggccggcggc atggaacccg
    14761 ggctcgcctg gcgcagcctc aggctgttcg agcaagaggt ctatccgcaa accgggctcg
    14821 actcgagccg cagcgagggc aaatgaacaa gcgtctccag gggcaggtcg cggtgatcac
    14881 cggcgcgggg cagggtgtgg ggcgcggcgt ggcgcggcgc atggccatcg agggcgccac
    14941 ggtggtggtc ggggagatca acacggagaa cggcaggcgc gttgcgggcg agtgtgagga
    15001 actgggcggg cgcggcatct tcgtgcacac cgacgtgacg cagcgcgact cgg
//
LOCUS       DAORUQ010000068        15049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3751_length_15049_cov_5.573629, whole genome shotgun sequence.
ACCESSION   DAORUQ010000068 DAORUQ010000000
VERSION     DAORUQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_15049_cov_5.573629"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="tdh"
                     /locus_tag="PLW13_07800"
     CDS             <1..829
                     /gene="tdh"
                     /locus_tag="PLW13_07800"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQY70315.1"
                     /translation="IVEIGQEVSGFRIGDRVTGEGHITCGYCRNCRAGRRHLCRNTIG
                     VGVNRPGAFAEYLVIPAFNAFRLSEEISDDLAAIFDPFGNAVHTALSFDLVAEDVLIT
                     GAGPIGIMAVAVARHCGARHVVITDVNEYRLGLARTMGATVAVNVNERSLESVMQELG
                     MTEGFDVGLEMSGVPSAFAAMLDAMNHGGRVAVLGIPPPDMRVDWTKIVFKGLTVKGI
                     YGREMFETWYRMAGLIHSGLDLEPLITHRLALADFQAGFEIMASGQSGKIILDWENA"
     gene            826..1248
                     /locus_tag="PLW13_07805"
     CDS             826..1248
                     /locus_tag="PLW13_07805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQY70316.1"
                     /translation="MSESPTETIEQIDSPIRSPFIEHLGIQFFRLGAGGVEVRLPVTP
                     ELLNSAAMVHGGVYATLADCALAAAIHGQVPPGQMVVTLDLSCRYLRAARAGVLRATA
                     RAVHVGRRTAVAEGEIWVDDVMQVKASGSFMIVPRAAT"
     gene            1312..2082
                     /locus_tag="PLW13_07810"
     CDS             1312..2082
                     /locus_tag="PLW13_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY70317.1"
                     /translation="MRVITFNANGIRSAADKGFFRWLAAQDADVICLQETRARIHQLN
                     DDHFFPPGYHCHYFDAQRPGYAGTAIFSRIEPDHVQKGLGWEPIDSEGRWIQADYKDI
                     SVVSLYMPSGSSGEERQAIKFALMDRLMPHLLALRRKKREFIICADWNICHREIDLKN
                     WRGNRKNSGFLPEERAWLDDLFGPVGYVDAFRVVNPHADQYTWWSNRGRARENNVGWR
                     LDYMAVTPGLRPKVRAESVYRDERFSDHAPLTMDFDWP"
     gene            2239..2589
                     /gene="yajC"
                     /locus_tag="PLW13_07815"
     CDS             2239..2589
                     /gene="yajC"
                     /locus_tag="PLW13_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQY70318.1"
                     /translation="MFFIPAAHAQAATPPAGGDPMISFAFMIGIFALFYFIAIRPQRK
                     RQKEHQELVNKLAVKDEVVTSSGILGRVTAVEQDFVVLKVAENVEIKLQKWAVQNVLP
                     KGTLKGVGAAGEKK"
     gene            2603..3664
                     /locus_tag="PLW13_07820"
     CDS             2603..3664
                     /locus_tag="PLW13_07820"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HQY70319.1"
                     /translation="MPAPQSGAGPAVAGVRSPPRLRLARLPTPFEPLDRLSALVGGPR
                     IWVKRDDLSDCAVSGNKIRKLEFTIAQALQENADTLVTCGGLQSNHCRATALLGARLG
                     LGVELVLRGEPSGIPDGNLFLDCLAGAAVHCYPARQYQQEHEALLAAHAKRLQAQGRR
                     PFVIPTGASDAIGVWGYVAACAELAGDFAQAGIDPRAVVCATGSGGTQAGLTAGLRLH
                     GLRAEAWGMAVCDDAAWFRNKVHADLASWQQRYGVALDTAALAVNVLDDYIGAGYAQA
                     GQDVFQTIGLVARTQGLILDPVYTGKAFHGMLHEIRHGRFRDEKDIVFVHTGGLFGLF
                     AQRGQALAGGALAVAGAEP"
     gene            3661..4434
                     /locus_tag="PLW13_07825"
     CDS             3661..4434
                     /locus_tag="PLW13_07825"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HQY70320.1"
                     /translation="MSFAQPPIPHPRFGSRIFRRRIRLENRDGAVIAGLEDNLHAARL
                     RLRHDGSRILAVEAQWLRQPNRNCGGAIAQLGRLAGQQLTGSRALFRACDDPRRHCTH
                     LHDLLGLAAAHALRPAPTREFDLAVPDPKDGVTFAQIRLDGEPVHRWRTDLLQILGPE
                     EARGGMFLRGFGAWSAARWGGDVLEAAAVLQMGIIISGSQRFDLEAMRRLHPDVALVS
                     RERVDTCYAYQAERIDTALPTQGTVRDFTDCPEELLRFL"
     gene            5188..6084
                     /locus_tag="PLW13_07830"
     CDS             5188..6084
                     /locus_tag="PLW13_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQY70321.1"
                     /translation="MPTEPGNKLVVAISSRALFDLDESHEIFERDGLEAYQRYQIEHE
                     EQILAPGDAFPLVHKLLGLNRRLPEARVEVILLSRNSADTGLRVFNSIQHHGLAISRA
                     AFCGGQSPWRYVSAFGCHLFLSTEAEDVRQALEQGVAAATFLPSRSSSGASEQLRFAF
                     DGDAVLFSDEAERVFKSQGLDAFTRSETASARQPLSGGPFKAFLAALQRLQHEFPQDD
                     CPIRTALVTARSAPAHERVVRTLRAWGIRIDESLFLGGLDKAEFLRAYGADVFFDDQR
                     GHCDSASQHVATGHVPHGVANR"
     gene            6169..7143
                     /gene="cysB"
                     /locus_tag="PLW13_07835"
     CDS             6169..7143
                     /gene="cysB"
                     /locus_tag="PLW13_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HQY70322.1"
                     /translation="MKLQQLRYIWEVARHDLNVSATAQSLFTSQPGISKQVRLLEDEL
                     GVEIFERSGKHLTRVTPAGEAILERAGDILRKVESIKQVAQEFRNDQKGTLSIATTHT
                     QARYALPPAIKVFMERYPEVSLHMHQGTPVQISEMAADGTVDFAIATEALELFSDLVM
                     MPCYTWNRCVIVPEGHPLASVKRLTIEDVAAYPIVTYVFGFTGRSRLDEAFHARRLTP
                     RVVFTATDADVIKTYVRLGLGIGIIARMAIDEQLDSGLVILDASHLFSPSVTKIGFRR
                     GTFLRGYMYEFIELFAPHLTREIVDKAWQLQGKAEVEALFEGVELPTY"
     gene            7214..7825
                     /gene="thrH"
                     /locus_tag="PLW13_07840"
     CDS             7214..7825
                     /gene="thrH"
                     /locus_tag="PLW13_07840"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HQY70323.1"
                     /translation="MEIACLDLEGVLIPEIWIEFAERTGIAELRATTRDIPDYDVLMK
                     QRLRLLDQHGLRLPDIQAVIDTMEPLPGAREFLDWLRERFQVLILSDTYYQFSAPLMR
                     QLGWPALFCHRLEVDATGRVTDYVLRQKDQKRQSVKAIKSLNFRVIAAGDSYNDTTML
                     AEADAGILFNAPANVIAEFPQFPSVEGYEALKEAFRRASIRAI"
     gene            complement(7812..9209)
                     /gene="pabB"
                     /locus_tag="PLW13_07845"
     CDS             complement(7812..9209)
                     /gene="pabB"
                     /locus_tag="PLW13_07845"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HQY70324.1"
                     /translation="MGAISILPLPYRIDGCDYFAQLRPLAGAVFLDSARVHGAGGRYD
                     LMSALPVCELRTHGAITRIMRRGTSVRSTADPFELVAQAIAEHVPCSPPPQGDWPFAG
                     GAMGWFGYDLGRRAARLATRDPPPQVADMQIGIYGWALLQDHERRESVALFTEAVDAT
                     IRRRLIDRLHGPREPAAAAFRLESPFVPNFDPVTYAAAFARVQRYIRDGDCYQVNLAQ
                     RLSARARGDAWSAYLQLRARMASPYSAFLANGQASVLSFSPERFLRAAGGRVETRPIK
                     GTRRRGATPEADREQARQLLASEKDRAENVMIVDLMRNDLGRSCRTGSVRVEQLCALE
                     SYANVHHLVSVVSGEPDPARGPTGLLADCFPGGSITGAPKIRAMQIIDELEPDPRSVY
                     CGSIGYISAHGTMDTSIAIRTLVCDGTQVHAWGGGGIVADSDAQTEYRESLTKIEPIL
                     EGLRAMSAAPALRSP"
     gene            9450..11177
                     /locus_tag="PLW13_07850"
     CDS             9450..11177
                     /locus_tag="PLW13_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY70325.1"
                     /translation="MSDENSAGYSLANPFGKEESLEFVVPGQIGYQPGLYEQTLAAGY
                     ALQQRPRRAAGVVNIERQHQKERMTIWERIQVLADGEPTVLFQNWGENLDGASLVTAI
                     IKVKGRDVAIYGHDFTVRAGSMDATNGRKLARLFELAGKRRIPLVGLNDSAGAYVPAG
                     VGGLDGYAEAFTALRKISGVVPSIMCMFGFNAGGGSYLPRQGSFVIQPRGTFFGLTGP
                     GVVKSVLGEDVTPDELGGPDVHSQSGVTDFVVEDEVSALRKVREILNYIPNNNAELAR
                     YQPTSDPVDRKTWDIDILLKKAFNSPTGFNTPFDVSIIIQQICDHGDFMEVQPERARN
                     TITAFGRMGGQVVGFVANNSAVASGQIDIAAAYKNARFIRFCNLYNIPVVFMEDTTGF
                     LPGREQETGGIVQAGRAMLDAIVDLRTPRFLVILRNAFGGAYASYNNYPTGADFVIAL
                     PTTRAAVMGPAGVEFVYKDELRAIRGQRQERLAAELKNLHEAGMAEEEASVVAKRHVD
                     AWIKESEAALATRYERELMNPNEALSLGSISQIVMPSDLRKVIAENLLFHLGHYTPEP
                     FAGVQREFH"
     gene            11212..14082
                     /locus_tag="PLW13_07855"
     CDS             11212..14082
                     /locus_tag="PLW13_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQY70326.1"
                     /translation="MRSSNDYYLNNPLVHRNRRLGHSASSWVRSFACEDMRPLIICRG
                     PIRREAMDVFDEMGIHDYGILLSEKDSIVYTNALAPELRTLTDPDRVHRVPDYTGASK
                     EERTQRIRQIIAIAKDNRYDSIFAGYGFMSEDEELVKAIEEAGLTFIGPCSRTVRGAG
                     FKDEAKRTALAVGVSVTPGIDNVTALTLLAKFPDAAALHALSEAEKLGLDAAVFADTN
                     PLEAQADAVLNAAYRKGMDLFSVEEMCAQLEKRVAEMYSRYPQNRIRLKAIGGGGGKG
                     QRILASPESFDGKTLEERIARAAARAPERAREILAEVKATGVGDNKNILIELNIETTR
                     HQEIQVIGNGVWATTLGGRDCSLQMHEQKLLEVSVTEEELALAIERAQAGGRVEEAKV
                     LQSDLAILRRMEDEAARFGAAVGLDSVSTFECIVERDHHFFMEMNTRIQVEHRVTELC
                     YGLRFSNPDDPDDSFVVESLVEAMVLLARHKTRMPKPDRVHRNNASVEARLNATNQAL
                     HPHAGGIIRNWSNAIEGEIRDDQGISLHNPDTDVFMAYTLAGAYDSNIALLLTVGNDR
                     LDSYQRLAEILRVTQLSGKDLATNLEFHYGLVNWFIGANINARPTTRFILPYLTAVGR
                     LREKASRIDLDYALAQVRRHRLAGVKDAGERKALAECIDRKQTLLLRPIQKLFAEPHM
                     LAGWLSRWRDGFSFAEGKVAFRRNPIELLSETYHYLNMDFSESTPPAYMIWDHDNAVL
                     ENALAFYGSVRELAGTSDWGATVQFLAQSKAPAGVPAQTWQAVRAAHAGYQAATELLL
                     VLPFVAQQTEFFGLSVNPDLSIHIPEQLLDPELQAEMAKVLVPPPAAKSDEILAASGG
                     MFYPRESPNSPEFVAEGQHFEQGDPLYIVEVMKMFNKIYAPFSGTIEKVLVAGEGVII
                     KKGQPLFKVVPDERIVEASPEEVRERRVSATAGFLATLEVTA"
     gene            14079..14489
                     /gene="mce"
                     /locus_tag="PLW13_07860"
     CDS             14079..14489
                     /gene="mce"
                     /locus_tag="PLW13_07860"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQY70327.1"
                     /translation="MITGLDHIAIAVPDLERAIRRFLEDFGLQYEGTEEVESARTTTA
                     FFPLPPTSIELVHPLRGEGPIAKYLEKKPGGIHHLCFRSDDIDADVARLKAKGYQFLS
                     EEPGPGAHDCRVIFIHPRSCDGVLIELSQPPAGH"
     gene            14594..>15049
                     /locus_tag="PLW13_07865"
     CDS             14594..>15049
                     /locus_tag="PLW13_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HQY70328.1"
                     /translation="MDGRLDWTASSRESPERSSLLGSLAALSCEALAPLLESTFGACE
                     TVFLELANTASSTYDEQRLLEFRRALSAKKSAVMADFAARITTEFTRLRQPEPGEPTG
                     DADSLELVAQEQIEKQLLVSGGVSRVRTSGARRWSNCTSAWWRSRLVNSA"
BASE COUNT         2517 a         5098 c         5086 g         2348 t
ORIGIN      
        1 aatcgtcgaa atcggccagg aagtgagcgg ttttcgcatc ggcgaccgcg tcaccggcga
       61 ggggcacatc acctgtggct actgccgcaa ttgccgcgcg gggcggcgcc acctgtgccg
      121 caacacgatc ggtgttggcg tgaaccgccc cggcgccttc gccgaatacc tggtgatccc
      181 ggcgttcaac gccttccggc tgagcgagga gatcagcgac gatctcgccg cgatattcga
      241 tcccttcggc aacgcggtgc acacggcgct gtccttcgac ctggtggccg aggacgtgct
      301 gatcaccggc gccgggccga tcggcatcat ggcggtggcg gtggcgcgcc actgcggggc
      361 gcgccacgtg gtgatcaccg acgtcaacga gtatcgcctc ggcctggccc gcacgatggg
      421 cgctacggtg gcggtcaacg tcaacgagcg cagcctggag agcgtcatgc aggaactcgg
      481 catgacggag ggcttcgacg tcgggctgga gatgtcgggc gtccccagtg cctttgccgc
      541 gatgctcgac gccatgaacc acggcgggcg cgtggcggtg ctcggtatcc cgccgccgga
      601 catgcgcgtg gactggacca agatcgtgtt caaggggctg acggtgaagg gcatctacgg
      661 gcgcgagatg ttcgagacct ggtaccgcat ggcggggttg atccattccg ggctggacct
      721 cgagcccttg atcacgcacc gcctggcgct ggcggatttc caggcgggct tcgagatcat
      781 ggcttccggg cagtccggca agatcatcct cgactgggag aacgcatgag cgaatcgcca
      841 accgaaacga tcgagcagat cgactcgccg atcaggagcc ccttcatcga gcatctgggc
      901 atccagttct tccgcctcgg cgccggcggt gtcgaagtgc ggctgcccgt cacgcccgag
      961 ttgctgaaca gcgccgcgat ggtgcacggc ggcgtctacg caacactggc cgattgcgcg
     1021 ctcgccgccg cgatccacgg ccaggtgccg ccgggccaga tggtggtcac gctggatctg
     1081 agctgccgct acctgcgtgc cgcgcgcgcc ggcgtgctgc gcgccacggc gcgcgcggtg
     1141 cacgtcggac ggcgcaccgc ggtcgccgaa ggcgagatct gggtcgacga tgtgatgcag
     1201 gtcaaggcca gcggcagttt catgatcgtg ccgcgcgccg ccacgtaggt cggtattcat
     1261 actgacaacg tgcgtaccgc caaaacacac cccgggagcc ccgcaccacg catgagagtc
     1321 atcaccttca acgccaacgg catccgctcg gccgccgaca agggtttctt ccgctggctg
     1381 gccgcgcagg acgccgacgt gatctgcctg caggaaaccc gcgcacgcat ccaccagttg
     1441 aacgacgacc atttctttcc gccgggctac cactgccatt acttcgacgc gcaacgtccg
     1501 ggctacgcgg gcacggcgat cttcagtcgt atcgagcccg accacgtgca gaaggggctg
     1561 ggctgggagc cgatcgacag cgagggacgc tggatccagg ccgactacaa ggacatcagc
     1621 gtggtgtcgc tgtacatgcc ctccggcagt tccggggagg agcgtcaggc gatcaagttc
     1681 gcgctgatgg acaggctgat gccgcacctc ctggcacttc ggcgcaagaa gcgcgagttc
     1741 atcatctgcg ccgactggaa catctgccac cgcgagatcg acctgaagaa ctggcgcggc
     1801 aaccgcaaga actccggttt cctgcccgag gagcgcgcct ggctcgacga tctgttcggg
     1861 ccggtgggct acgtggacgc cttccgcgtg gtgaacccgc acgcggacca gtacacctgg
     1921 tggtcgaacc gcgggcgcgc gcgcgagaac aacgtggggt ggcgactgga ctacatggcc
     1981 gtgacgccgg gcttgcggcc gaaggtccgg gccgagtcgg tctaccgcga cgagcgcttc
     2041 tccgaccacg caccgctcac gatggacttc gattggccct agtagggccg gattcatccg
     2101 gacacccgat ccttcggaca gggtcctacc aggagttgtg agtgcgcgcc aaagccttta
     2161 aagtcccgcc cagaacttga tgttgcatca gaacaacgtt taggtttcgt ttgtattcaa
     2221 ttgattccag gagtttttat gttcttcatt ccagccgccc acgcccaggc cgccacgcca
     2281 cccgccggcg gcgacccgat gatctccttc gccttcatga tcggcatctt cgcgctgttc
     2341 tatttcattg cgatccgccc gcaacgcaag cgccagaagg aacaccagga actggtcaac
     2401 aagctcgcgg tcaaggacga ggtggttacg tcgagcggca tcctcgggcg cgtcaccgcg
     2461 gtcgagcagg acttcgtggt cctgaaggtg gccgagaacg tcgagatcaa gttgcagaag
     2521 tgggcggtac agaacgtgct gcccaagggc acgttgaagg gcgttggcgc ggccggcgag
     2581 aaaaagtagg agcgataccg ccatgcccgc cccgcagtcc ggcgccgggc ccgccgtggc
     2641 gggcgtccgc tcgccgccgc ggctgcggct ggcacgcctg ccgacgccgt tcgagccgct
     2701 cgaccggctg agcgcgctgg tcggcggacc gcggatctgg gtcaagcgcg acgacctgag
     2761 cgattgcgcc gtgagcggca acaagatccg caagctcgag ttcacgatcg cgcaggcgct
     2821 gcaggagaac gcggacacgc tcgtcacctg cggcggcctg cagtccaacc actgccgcgc
     2881 caccgccttg ctcggcgcgc gcctgggcct gggggtcgag ttggtgctgc gcggcgagcc
     2941 ctcgggcatt cccgacggca acctgttcct cgactgcctc gcgggcgcgg cggtgcactg
     3001 ctatccggcg cggcagtacc agcaggagca cgaggcgctg ctcgcggccc acgccaagcg
     3061 cttgcaagcg caggggcgac gcccgtttgt gatccccacc ggcgccagcg acgcgatcgg
     3121 cgtctggggg tacgtggcag cctgcgccga gctcgccggg gacttcgcgc aggcgggcat
     3181 cgatccgcgt gcggtggtct gtgccaccgg gtcgggcggc acgcaggcgg ggctcacggc
     3241 cgggcttcgc ctgcacgggc tgcgggccga agcctggggc atggccgtct gcgacgatgc
     3301 ggcctggttc cgcaacaagg tgcacgcgga cctcgcgtca tggcagcagc gctacggtgt
     3361 ggcgctggat acggcggcgc tggcggtgaa cgtgctcgat gactatatcg gggcgggcta
     3421 cgcgcaggcc ggccaggacg tgttccagac catcggcctc gtcgcgcgca cgcaagggct
     3481 gatcctggat cccgtctaca ccggcaaggc cttccacggc atgctgcacg aaattcgcca
     3541 cgggcgtttc cgcgacgaga aggacatcgt gttcgtgcac acgggcgggt tgttcggcct
     3601 gttcgcgcag cgcgggcagg cgctggcggg cggcgcgctg gcggtggcgg gcgcggagcc
     3661 gtgagcttcg cgcaaccgcc gatacctcat ccccgtttcg gcagccgcat attccgccgc
     3721 cggatccgtc tcgagaaccg cgacggcgcg gtgatcgcgg ggctggagga caacctgcat
     3781 gccgcgaggc tgcggttgcg ccacgacggc agccgcatcc tcgccgtcga ggcgcagtgg
     3841 ctgcgccagc ccaaccgcaa ctgtggcggg gcgatcgcgc aactgggacg gctcgccggc
     3901 cagcaactca ccgggagccg cgcgctcttt cgcgcctgcg acgatccgcg ccgccattgc
     3961 acccatctgc acgacctgct ggggctcgca gccgcgcacg cgctgcgccc ggcgccaacg
     4021 cgggagttcg acctggcggt cccggacccg aaggacggcg tcaccttcgc gcagatacgc
     4081 ctggacggcg aaccggttca ccgctggcgc accgacctgc tgcagatcct cgggcccgag
     4141 gaagcccgtg gcggaatgtt cctgcgcggc tttggtgcat ggtccgccgc gcgctggggc
     4201 ggggatgtgc tggaggcggc cgcggtgctg cagatgggca tcatcatctc gggctcgcag
     4261 cgcttcgacc tcgaggcgat gcgccggctg catcccgatg tcgcgctggt ctcgcgcgag
     4321 cgcgtcgata cctgttatgc ctaccaggcc gaacgcatcg ataccgcgct gccgacccag
     4381 ggcaccgtgc gggacttcac cgactgcccg gaggagttgt tgcggttcct gtgaggcctc
     4441 gcgttgtcgc cggctagccg ggcgttgtgc gacgcgctct tcagccgcgc accggttatc
     4501 ggagcgtaca cggaaatgtc ccgctgaagc gggacctacg agtattgcgc gagcactgcc
     4561 cgcaccccgc gccagccatg cgctgccgaa caggttcagg tggttgagca ggtggtagag
     4621 gttgtagagc ggcatgcgct cctcccagcc gggctcgagc ggacgcatct ccaggtaggc
     4681 atcgtagaag gcgtcgtcga agccgccgag caggcgcgtc gtcgccaggt ccgcctcggg
     4741 ccagccccag taggccgccg gatcgaccag caccgcctcg ccgttggtgt ccgcgaacac
     4801 gttgccatgc cacaggtcgc cgtgcagcag ggccggcgcc tgcaccggta ccaggtcctg
     4861 gagccgcccg gcgatggcct cgacgcgatg cagttccagc gccgccagca ggccggcggc
     4921 gtgggctcgc ccggcctgga acagcaggcg atggttcgcg aaaaaggcgt gcccgtcggt
     4981 gtcacgcggg ttgggctgcg gcgtggtgcc gcaccagttg tccatcagga atccgaagct
     5041 gggcgccgca gtgcggtgct ggcgcgccag cgcgcgggcg agcccgcgcg agtaggcgcc
     5101 gccggcggtg cccgccgcga cgaactgttc gcatggcgct cccggtggtt acaatgcccc
     5161 acgttcacgc gggatcgcgc gcaggttatg ccaacagaac cgggaaataa actcgtcgtc
     5221 gcgatctcct cgcgcgccct gttcgacctc gacgagagcc acgagatctt cgagcgcgac
     5281 ggcctcgagg cctaccagcg ctaccagatc gagcacgagg agcagatcct cgcaccgggc
     5341 gacgccttcc ctctggtgca caagctgctc ggtctcaacc ggcgcttgcc ggaggcgcgc
     5401 gtcgaggtga tcctgctctc gcgcaacagc gccgacacgg gtctcagggt cttcaacagc
     5461 atccagcacc acgggctggc gatctcgcgt gcggcgttct gcggcggcca gagtccgtgg
     5521 cgctacgtga gcgccttcgg ctgccacctg ttcctgtcga ccgaggccga ggacgtgcgc
     5581 caggcgctgg agcagggtgt cgccgcggca accttcctgc cctcgcgctc tagcagcggc
     5641 gccagcgagc agctgcgttt cgccttcgac ggcgatgcgg tgctgttctc cgacgaggcc
     5701 gaacgggtct tcaagagcca gggcctggac gccttcacgc gcagcgagac ggcgtcggcg
     5761 cgccagcccc tgagcggcgg acccttcaag gcctttctcg ccgcgttgca gcggctgcag
     5821 cacgagttcc cgcaggacga ctgtccgatc cgcaccgcgc tggtcacggc gcgctcggcg
     5881 ccggcgcacg aacgcgtcgt gcgcacgctg cgcgcctggg ggatccgcat cgacgaatcg
     5941 ctgttcctcg gcggtctcga caaggcggag ttcctgcgcg cctacggtgc cgacgtgttc
     6001 ttcgacgacc agcgcgggca ttgcgactcg gccagccagc acgtcgccac cggccacgtg
     6061 cctcatggcg tggcgaaccg ctgaggcctt gccgtggtgg cgatcgcgcc tgaatggggc
     6121 tatcctgcgc gcctcccggc tccgctcccg cgctgacccc gaacccgcat gaaactccag
     6181 cagcttcgct acatctggga ggtcgcccgc catgacctca acgtctccgc gaccgcgcag
     6241 agcctgttta cctcgcagcc gggcatcagc aagcaggtgc ggctgctcga ggacgaactc
     6301 ggcgtcgaga tcttcgagcg aagcggcaag cacctcacac gcgtgacgcc ggccggcgag
     6361 gcgatcctcg agcgcgccgg cgacatcctg cgcaaggtcg aatcgatcaa gcaggtggcg
     6421 caggagttcc gcaacgacca gaaaggcacg ctgtcgatcg ccaccacgca cacgcaggcg
     6481 cgctatgcgc tgccgccggc gatcaaggtg ttcatggagc gctaccccga ggtgtcgctg
     6541 cacatgcacc agggcacgcc ggtgcagatt tccgagatgg cggccgatgg cacggtggat
     6601 ttcgccatcg ccaccgaggc gctggaactc ttcagcgatc tggtgatgat gccctgctac
     6661 acgtggaacc gctgcgtgat cgtgcccgag ggccatccgc tggcgagcgt gaagcgtctc
     6721 accatagagg atgtggccgc gtacccgatc gtgacctacg tgttcggctt cacggggcgc
     6781 tcgcgtctcg acgaggcctt tcacgccagg cggctcacgc cgcgcgtggt gttcacggcc
     6841 accgatgccg acgtgatcaa gacctacgta cgcctcgggc tcggcatcgg gatcatcgcg
     6901 cgcatggcga tcgacgagca gctcgacagc ggactggtga ttctggatgc cagccacctg
     6961 ttcagtccca gcgtcaccaa gatcggtttc cgccgcggca ccttcctgcg cggctatatg
     7021 tacgaattca tcgagctgtt cgcgccgcac ctgacgcgcg agatcgtcga caaggcctgg
     7081 cagctgcagg gcaaggccga ggtcgaggca ctgttcgagg gcgtcgagct gccgacttac
     7141 tgatcgcgcc ggtgctggca ggagcgggcc atgcccgcga gaggatgttg ttctttagcc
     7201 cctggagacc cacgtggaaa tagcctgtct cgacctcgaa ggtgtactga tcccggagat
     7261 ctggatcgaa ttcgccgagc gcaccggcat tgccgaactg cgcgccacca cgcgcgacat
     7321 tcccgactac gacgtgctga tgaagcagcg cctgcgcctg ctcgaccagc acgggctgcg
     7381 cctgcccgac atccaggccg tgatcgacac gatggagccg ctgccgggcg cgcgcgagtt
     7441 cctcgactgg ctgcgcgagc gattccaggt cctgatcctc tcggacacgt actaccagtt
     7501 ctcggcgccc ctgatgcgcc agctgggatg gccggcgctg ttctgtcacc gtctcgaggt
     7561 cgacgcgacc gggcgcgtaa cggactacgt gctgcgccag aaggaccaga agcgccagtc
     7621 ggtgaaggcg atcaagtcgc tgaacttccg cgtgatcgcg gccggcgatt cctacaacga
     7681 caccacgatg cttgccgagg ccgatgccgg gatcctgttc aacgcgccgg ccaacgtgat
     7741 cgcggagttt ccgcagttcc cgtcggtcga aggttacgag gcgctgaagg aagccttccg
     7801 gcgcgccagc atcagggcga tctgagcgcc ggcgcggcgc tcatcgcgcg caggccctcg
     7861 agaatcggtt cgatcttggt gagcgactcg cggtactccg tctgcgcatc ggagtccgcg
     7921 acgatgccgc cgccgcccca ggcatgcacc tgcgtgccgt cgcagaccag cgtgcgtatc
     7981 gcgatgctgg tgtccatggt gccgtgcgcc gagatgtagc cgatagagcc gcagtagacg
     8041 ctgcgcgggt cgggctccag ctcgtcgatg atctgcatcg cgcggatctt cggcgcaccg
     8101 gtgatcgagc cgcccgggaa acagtcggcg agcagcccgg tcgggccgcg cgccggatcg
     8161 ggctcgccgc tcaccacgct gaccaggtga tgcacgttgg catagctctc cagcgcgcac
     8221 agttgctcca cgcgcacgct cccggtgcgg cacgacctgc cgagatcgtt gcgcatcagg
     8281 tcgacgatca tcacgttctc ggcgcggtcc ttctccgagg ctagcagttg ccttgcctgc
     8341 tcgcggtcgg cctcgggcgt ggcgccgcgg cgccgcgtgc ccttgatggg ccgcgtctcg
     8401 acgcgtccgc cagccgcgcg caggaagcgc tccggcgaga agctgagcac gctggcctga
     8461 ccattggcca ggaacgcgga gtagggcgag gccatgcgcg cacgcagctg caggtaggcc
     8521 gaccaggcat cgccgcgcgc ccgcgcgctc agccgctgcg cgagattcac ctggtagcaa
     8581 tcgccgtcgc ggatgtagcg ctgcacccgc gcgaacgccg cggcataggt gacgggatcg
     8641 aagttcggta cgaacgggga ctcgagccgg aaagccgccg ccgcgggctc ccggggcccg
     8701 tgcaggcgat cgatcaggcg gcgtcggatc gtcgcgtcga ccgcttccgt gaacagcgcc
     8761 acgctctcgc gacgttcgtg atcctggagc agcgcccagc catagatccc gatctgcatg
     8821 tcggcgacct gcgggggcgg gtcgcgcgtc gcgagccgcg ccgcgcgtcg cccaaggtcg
     8881 tagccgaacc agcccatcgc cccaccggcg aagggccaat cgccctgcgg cggcggggaa
     8941 cacggcacgt gctccgcgat tgcctgggcc accagttcga aggggtcggc ggtgctgcgc
     9001 acgctcgtgc cgcggcgcat gatgcgcgtg atcgcgccgt gcgtgcgcaa ctcgcacacg
     9061 ggcaacgccg acatcaggtc gtagcgtccg ccggcgccgt gcacgcgcgc gctgtcgagg
     9121 aaaaccgcgc ctgcgagggg ccgcaactgc gcgaaatagt cgcagccgtc gatgcgatac
     9181 ggcagcggga ggatcgaaat tgcgcccacg ctcgaagttt cccgtgccac cggctttcaa
     9241 aactattgat atcatagcgc taagtcatta atatgtcgcg gttttttaac tccgtaggcg
     9301 catcgcgccg cggcaactcc cctgccggca ggggagagcg cggcggggcg gtgcatggca
     9361 cacttgcggc gtatcgccgt gcgcgaggct ttccgctcct gcgcgcggcg tgcgtgtgca
     9421 cgattcagac acccgagagg cactgctaga tgagcgacga gaacagcgcg ggatactccc
     9481 tggcgaatcc cttcggcaag gaggaaagcc tcgagttcgt ggtcccggga cagatcggtt
     9541 accagccggg gttgtacgag cagacgctcg ccgccggcta tgcgctgcag cagcgcccgc
     9601 gtcgcgccgc cggggtcgtc aacatcgagc gccagcacca gaaggagcgc atgacgatct
     9661 gggagcgcat ccaggtgctc gccgacggcg agccgacggt gctgttccag aactggggcg
     9721 agaatctcga cggtgcctcg ctggtgacgg cgatcatcaa ggtcaagggg cgcgacgtcg
     9781 ccatctacgg ccacgatttc acggtacgcg ccggctcgat ggacgccacc aacggccgca
     9841 agctcgcccg cctgttcgag ctcgccggca agcgccgcat cccgctggtc ggactgaacg
     9901 acagtgccgg tgcctacgtg ccggcgggcg tgggcggcct cgacggctac gccgaggcct
     9961 tcacggcact gcgcaagatc agcggcgtgg tgcccagcat catgtgcatg ttcggcttca
    10021 acgccggcgg cggctcctac ctgccgcgcc agggcagctt cgtgatccag ccgcgcggca
    10081 ccttcttcgg cctcaccggc ccgggcgtgg tcaagtcggt gctcggcgag gacgtcacgc
    10141 cggacgaact cggcggcccc gacgtgcact cgcagtcggg tgtcaccgac ttcgtggtgg
    10201 aggacgaggt cagcgcgttg cgcaaggtgc gcgagatcct caactacatc ccgaacaaca
    10261 acgcggagct ggcacgctac cagccgacct cggacccggt cgaccgcaag acctgggaca
    10321 tcgacatcct gttgaagaag gccttcaact cgccgaccgg cttcaacacg ccctttgacg
    10381 tcagcatcat catccagcag atctgcgacc acggcgactt catggaagtg cagcccgagc
    10441 gcgcgcgcaa caccatcacg gccttcggcc gcatgggcgg gcaggtggtc ggcttcgtcg
    10501 ccaacaacag cgccgtggcg tcggggcaga tcgacatcgc cgcggcctac aagaacgcgc
    10561 gcttcatccg cttctgcaat ctctacaaca tcccggtggt cttcatggag gacaccacgg
    10621 gcttcctgcc cgggcgcgag caggaaaccg gcggcatcgt gcaggccggt cgcgcgatgc
    10681 tcgacgcgat cgtcgacctg cgcacgccgc gcttcctggt gatcctgcgc aatgccttcg
    10741 gcggcgccta cgcctcgtac aacaactatc cgaccggcgc ggacttcgtg atcgcgctgc
    10801 ccacgacgcg cgcggccgtg atggggccgg caggcgtgga gttcgtctac aaggacgagc
    10861 tgcgcgcgat tcgcggccag cgccaggagc gtctcgccgc ggagctgaag aatctgcacg
    10921 aagccggcat ggccgaggaa gaggcaagcg tcgtggcgaa gcgccacgtc gatgcctgga
    10981 tcaaggagag cgaggcggca ctcgcgaccc gctacgagcg cgagctcatg aacccgaacg
    11041 aggcgctcag cctcggctcg atctcgcaga tcgtgatgcc ttcggacctg cgcaaggtca
    11101 tcgcggagaa cctgttgttc cacctcggac actacacgcc ggaacccttc gcgggcgtcc
    11161 agcgcgagtt ccattgagcc acttaccagc catcttcgcg gaaaacaacc agtgcgcagc
    11221 agcaacgact actacctgaa caatcccctg gtgcatcgca accgccggct cgggcacagc
    11281 gcctcgagct gggttcgcag ctttgcctgc gaggacatgc gcccgttgat catctgccgg
    11341 gggccgatcc gccgcgaggc gatggacgtc ttcgacgaga tgggcatcca cgactacggc
    11401 atcctgctct ccgagaagga ctccatcgtc tataccaacg cgctggcgcc cgagctgcgc
    11461 acgctgaccg accccgaccg cgtgcaccgc gtgcccgatt acacgggcgc gagcaaggag
    11521 gagcgtacgc agcgcatccg ccagatcatc gcgatcgcga aggacaatcg ctacgactcc
    11581 atcttcgccg gctatggctt catgtccgag gacgaggagc tggtgaaagc catcgaggag
    11641 gccggcctca cctttatcgg accgtgcagc cgcaccgtgc gcggcgccgg cttcaaggac
    11701 gaggccaagc gcaccgcgct cgcggtcggc gtcagcgtca ccccgggcat cgacaacgtc
    11761 acggcgctga cgctgctcgc caagttcccc gacgcggcgg cgctgcacgc gctgtccgag
    11821 gccgagaagc tcggactgga tgcagcggtc ttcgccgata ccaacccgct cgaggcccag
    11881 gccgacgcgg tgctcaacgc cgcctaccgc aagggcatgg acctgttcag cgtcgaggag
    11941 atgtgcgcgc agctcgagaa gcgcgtcgcc gagatgtact cgcggtaccc gcagaaccgc
    12001 atccgcctga aggcgatcgg cggcggcggc ggcaagggcc agcgcatcct cgcctcgccg
    12061 gagtccttcg acggcaagac actcgaggag cgcatcgcca gggccgcggc acgcgcgccg
    12121 gagcgtgcgc gcgagatcct cgcggaggtg aaggccaccg gcgtgggtga caacaagaac
    12181 atcctgatcg agttgaacat cgagaccacg cgtcaccagg aaatccaggt gatcggcaac
    12241 ggcgtctggg ccacgacgct aggcgggcgc gactgctcgc tgcagatgca cgagcagaag
    12301 ctgctcgagg tctcggtgac cgaggaggag ctggcgctcg ccatcgagcg cgcgcaggct
    12361 ggcggccgcg tcgaggaggc gaaagtgctg cagagcgacc tcgcgatcct gcgccgcatg
    12421 gaggacgaag ccgcgcgctt cggcgcggcg gtcggtctcg actcggtctc gaccttcgag
    12481 tgcatcgtcg agcgcgacca tcacttcttc atggagatga acacgcgcat ccaggtggag
    12541 caccgcgtca ccgagctctg ctatggcctg cgcttcagca acccggacga cccggatgac
    12601 agcttcgtgg tcgagtcgct ggtcgaggcg atggtgctgc tggcgcggca caagacgcgc
    12661 atgccgaagc ccgatcgcgt gcaccgcaac aacgcctccg tcgaggcgcg cctcaacgcg
    12721 accaaccagg cgctgcaccc gcacgccggc ggcatcatcc gcaactggtc caacgccatc
    12781 gagggcgaga tccgcgacga ccagggcatc agcctgcaca acccggacac cgacgtgttc
    12841 atggcctaca cgctggccgg ggcgtacgac tcgaacatcg cgctgctgct cacggtcggc
    12901 aacgaccgcc tcgacagcta ccagcgcctc gcggagatcc tgcgcgtcac gcagctctcg
    12961 ggcaaggatc tcgcgaccaa cctcgagttc cactacggtc tcgtgaactg gttcatcggc
    13021 gccaatatca atgcgcgtcc caccacgcgt ttcatcctgc cgtacctcac tgccgtgggg
    13081 cgcctgcgcg agaaggcgag ccgcatcgac ctggactacg cgctcgcgca ggtgcgccgg
    13141 caccgcctcg cgggcgtcaa ggacgccggc gagcgcaagg cgctcgccga gtgcatcgac
    13201 cgcaagcaga cgctgttgct gcgcccgatc cagaagctct ttgccgagcc gcacatgctg
    13261 gccggctggc tcagccgctg gcgcgacggc ttcagctttg ccgagggcaa ggtggcgttc
    13321 cggcgcaacc ccatcgagct gctgtcggag acctatcact acctgaatat ggacttcagc
    13381 gagtcgacgc cgccggcgta catgatctgg gatcacgaca atgcggtgct cgagaatgcg
    13441 ctggccttct acggctcggt gcgcgaactc gcggggacct cggactgggg cgcgacggtg
    13501 cagttcctgg cgcagtccaa ggcgccggcc ggcgtgccgg cgcagacctg gcaggcggtg
    13561 cgcgcggcgc acgcgggcta ccaggccgcg accgagctgc tgctggtgct gcccttcgtg
    13621 gcgcagcaga cggagttctt cgggctcagc gtgaacccgg acctcagcat ccacatcccg
    13681 gagcaattgc tcgaccccga gctgcaggcc gagatggcga aggtgctggt accgccgccc
    13741 gctgcgaaat ccgacgagat cctcgcggcg tccggcggca tgttctaccc gcgcgagtcg
    13801 ccgaactcgc cggaattcgt cgccgaggga cagcacttcg agcagggcga tccgctctac
    13861 atcgtcgagg tgatgaagat gttcaacaag atctacgcac cgttcagcgg cacgatcgag
    13921 aaggtgctgg tcgcgggcga aggcgtgatc atcaagaagg ggcagccgct gttcaaggtc
    13981 gtgcccgacg agcgcatcgt cgaggcgtcg cccgaagagg tgcgcgagcg ccgggtcagc
    14041 gcgactgcag gattcctggc cacgctggag gtaacggcat gatcaccggg ctggaccata
    14101 tcgctatcgc cgtgcccgac ctggagcgcg cgatccgccg cttcctcgag gacttcggcc
    14161 tgcagtacga gggcaccgag gaggtcgagt ccgcgcgcac caccacggcc ttcttcccgc
    14221 tgccgccgac cagcatcgag ctggtgcacc cgctgcgcgg cgaaggaccg atcgcgaagt
    14281 acctggagaa gaagccgggc ggcatccacc acctgtgctt tcgcagcgac gatatcgatg
    14341 ccgacgtggc acgattgaag gcaaagggct accagttcct cagcgaggaa ccggggccgg
    14401 gcgcgcacga ctgccgcgtg atcttcatcc accccaggag ctgcgatggc gtgctgatcg
    14461 agctgagcca gccgcccgcc ggccattgag gcgcccgttc ggggcagggc ttgcgcacgc
    14521 tgtgatgccc tgcacgcttc gggcatccgc accggtctag aattttccgg ccgggcacag
    14581 gagcaccgat tgcatggatg gacggctgga ttggacggcg agcagccggg agtcgccgga
    14641 gcgctcatcg ctgctgggtt cgctggcggc gttgagctgc gaagcgctcg cgccgctgct
    14701 cgaaagcact ttcggagcct gcgagaccgt gtttctcgag ctggcgaaca ctgcctccag
    14761 cacgtatgac gagcagcgcc tgctcgagtt ccggcgcgcg ctgagcgcga agaaatcggc
    14821 ggtaatggcg gacttcgcgg cccgtatcac gaccgaattc acccgcctgc ggcaaccgga
    14881 acccggcgaa cctaccggcg acgcggactc gctggagctt gtcgcacagg aacagatcga
    14941 gaagcagctg ctggtctccg gtggcgtctc gcgggtgcga acgagtggcg cgcggcgctg
    15001 gagcaattgc acgagcgcat ggtggcgatc gcgcctcgtg aattcagca
//
LOCUS       DAORUQ010000069        14973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3785_length_14973_cov_6.460920, whole genome shotgun sequence.
ACCESSION   DAORUQ010000069 DAORUQ010000000
VERSION     DAORUQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3785_length_14973_cov_6.460920"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..829)
                     /locus_tag="PLW13_07870"
     CDS             complement(98..829)
                     /locus_tag="PLW13_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY70329.1"
                     /translation="MSESTGLCLRVLRIGALACLPLLGACAGQFAGSTHTPPRVDVPE
                     PGNGRAAPANRAPATAQPQTSAVKVYPAPTASTAAQASAYPAAQPPQTGQALPATYPG
                     APAPASTMPLPGATAPASEDSFTIEAPAVAGPAGSAGGAGQIDLQAPPPSANTAVNTL
                     LAQAATERRGGNLDAAIAAAERALRIAPADPAVYCELATLRLERGDYALAEQLARKGL
                     SYQPDAQMQQRLNAIIERARRGKTG"
     gene            complement(899..3268)
                     /gene="mrcB"
                     /locus_tag="PLW13_07875"
     CDS             complement(899..3268)
                     /gene="mrcB"
                     /locus_tag="PLW13_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HQY70330.1"
                     /translation="MVLLRLLAAAAVVGVLAFMYLDAYIQREFSGKKWAVPAMVYGRP
                     LELYAGAPVGPRALGAELETLGYRAAGGAGSAGTYSVSGSTVSIATRGFRFWDGEEPA
                     RRLRVAFAGNSVQAVHDEQGAEVALARLEPVHIGGIYPAHNEDRILVRVSDVPPLLIR
                     TLMAVEDQNFDSHHGVSPKGIARAMWVNVSSGALEQGGSTLTQQLVKNFFLTRERTIT
                     RKLMELALALVMELRYSKEEILEAYLNEIYLGQDGHRAIHGFGLASHYYFNRPIAELG
                     APQIALLVGMVRGPSYYDPWRHPERSRERRDTVLALMVDQQVIDAAQAAQIAQMPLGV
                     GDREQARSKFYPAYLDLVRRQLRRDYSEEVLSSEGLQIFTGLDPAVQRAAEASLQETL
                     AKIESDFKARGKPQQGLEGAVIVTRVDSGEVLAVVGGRNSRVAGFNRALDALRPVGSL
                     MKPAVYLTALEDGRYTLLSPLDDGPVAHKGANGQVWRPRNYDREDHGMVRLHRALAQS
                     YNQSTARLGLELGIERVVDTIHRLGVDREVPALPSVALGAVALSPIEVAGMYQTIASG
                     GFSSPLSAIREVMDASGEPLTRYPVAVEQKVRQSAVYLLEYALKEVVSAGTARSAYNR
                     LPAGLVVAGKTGTTDDQRDSWFAGYSGDLLAVVWIGRDDNGTTALTGASGALPAWTGL
                     MAAVSREPRRSRAPGGITETWVDEAGGGLSGEGCPGARLMPFMNGTEPDYEAACTGSG
                     LRWPWSGGKPAAEPGAAAEPGAAAQPDSAPEQEEKARDRWWKRWFGGDR"
     gene            3527..4537
                     /locus_tag="PLW13_07880"
     CDS             3527..4537
                     /locus_tag="PLW13_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HQY70331.1"
                     /translation="MKKKYNVYGVGAALVDTEIEVDDAFLGSAGIDKGVMTLVDEPRQ
                     DRLLELLSTHLVASRRASGGSAANSVIATSCFGGRAFYSCKVADDENGRFYLADMARA
                     GVDCNVCAGRESGVTGKCLVMITPDAERTMNTFLGISSTLSAQNLDAAALAASEFVYV
                     EGYLVTSDSGRAAAIAARELAERSGVRTALSFSDPGIVAHFRDGLAQMLGNGVDLLFC
                     NLAEAQEWTGEQRLEDAAEGLRKVARSFAITLGGEGALVFDGRHYHRIAPYPVKPIDT
                     NGAGDMFAGAYIYGITNGFDAVAAGRLANRAAAELVTHYGPRLPAAAHRRLLDEFDRE
                     QG"
     gene            complement(4601..5557)
                     /locus_tag="PLW13_07885"
                     /pseudo
     CDS             complement(4601..5557)
                     /locus_tag="PLW13_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
     gene            complement(5654..6112)
                     /locus_tag="PLW13_07890"
     CDS             complement(5654..6112)
                     /locus_tag="PLW13_07890"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQY70332.1"
                     /translation="MTYSLPQRAVLLLAALAALAALAGVGPAYAAALDITGAWLRQPP
                     PGQTGAAIYMELHNGGTAPLAVVGARVEGAASAAIHGHSHADGMMRMYPVPRLEIAPG
                     ADLSLQPGGYHLMVGGMGSALQPGALLPFCLRLEDGVEQCAEARVRKIGE"
     gene            6264..8027
                     /locus_tag="PLW13_07895"
     CDS             6264..8027
                     /locus_tag="PLW13_07895"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQY70333.1"
                     /translation="MLRLLLIAALLWGANIATANQDALPGASRDAGMLGGSAHAFLRV
                     DEAYALRAELRDAQVLVQWDIAPGYYLYKERIGFKWKGKDAAEAIVVTLPAGEAHEDA
                     YLGATEVYRGTLAVTLPLPGSEPALLEVKSQGCADAGLCYPPRTRVFAVDPVTGSLDE
                     TEPEQIPVTAAQSAAPAPATGPADLAKLLWMALLAALGGMILNLMPCVFPVLSLKVLG
                     IAAKGGNGHGKALHGLAYAAGVVLSFVAVAGLLLALRGAGAAIGWGFHLQSPAFVAAL
                     AYLFVVMGLALSGMINLGSGVMGSGEALARQGGYRGTFFTGVLATVVASPCSAPFMGT
                     ALGFAMTQPPVYALAIFAALGLGMAAPFVLLTLSPALLRRLPHPGPWMDGFKQFLAFP
                     LYAAAVWLLWVLGNQTGVNGMAAVTGGCVLLALGLWCWEHAKLHRRPLPLRLLGALIC
                     VLALLVLRSPLLDERPAVRTDSTGAWEDYSPARLEELRAAGTPVFVNVTADWCITCLA
                     NERIALASEEVLAAFRDRGIVYLKGDWTNSDPELTALLQAHGRSGVPLYLFYPAAAGA
                     QATLLPQLLTPSIVLEFINTR"
     gene            8176..8622
                     /gene="aroQ"
                     /locus_tag="PLW13_07900"
     CDS             8176..8622
                     /gene="aroQ"
                     /locus_tag="PLW13_07900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQY70334.1"
                     /translation="MATILVLHGPNLNLLGTREPETYGSVTLEQINQRLAGIAVGAGH
                     HLQTLQSNAEHELIERIHAARSEGIGFIVINPGGFTHTSVALRDALLGVAVPFIEVHI
                     SNVHAREPFRRHSYFSDVAVGVICGLGPIGYELALQAAIARLTPAT"
     gene            8642..9100
                     /gene="accB"
                     /locus_tag="PLW13_07905"
     CDS             8642..9100
                     /gene="accB"
                     /locus_tag="PLW13_07905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQY70335.1"
                     /translation="MDIRKIKTLIELLEGSDVTEIEICEGEESVRISRGHAQPVYAAP
                     PMAMPFTAAHPAPAAPAAPAPMAPAAAAEPAGHLVRSPMVGTFYRSPSPGAKAFVEVG
                     QSVKAGEVICIIEAMKMMNHIESDKAGTISAILVEDGQPVEFDQPLFSIV"
     gene            9136..10476
                     /gene="accC"
                     /locus_tag="PLW13_07910"
     CDS             9136..10476
                     /gene="accC"
                     /locus_tag="PLW13_07910"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQY70336.1"
                     /translation="MLEKVVIANRGEIALRILRACRELGIRTVAVHSQVDRDLMHVRL
                     ADEAVCIGPNPSGQSYLNVPSIISAAEITDAVAIHPGYGFLAENADFAEQVEKSGFVF
                     IGPTADVIRLMGDKVSAKDAMKAAGVPTVPGSDGALPEDGDEILAMARAVGYPVIIKA
                     AAGGGGRGMRVVHSEAALLNSVFVTRNEAQAAFGDGTVYLEKFLENPRHVEFQVLCDG
                     KGGAIHLGDRDCSLQRRHQKVLEEAPAPGIPDALKRAIAERCVQACLKIGYRGAGTFE
                     FLYENGQFFFIEMNTRVQVEHPVTEAITGIDIVREQLLIAGGQPLSIRQEDVHFRGHA
                     FECRINAEDPKTFAPSPGKVRLFHAPGGNGIRVDSHLYSGYSVPPNYDSLIGKVISWA
                     EDRHGALMRMRNALDEIVVEGIRTNIPLHQQLVRDEAFHRGGVSIHYLEKKLQK"
     gene            10483..11388
                     /gene="prmA"
                     /locus_tag="PLW13_07915"
     CDS             10483..11388
                     /gene="prmA"
                     /locus_tag="PLW13_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQY70337.1"
                     /translation="MGWLQLRLVATADRVEVVEDALLALGALSVTLADHRDVPILEPG
                     VGETPLWPEVDLVALFDKDTDTAAVGAALAELLGEAARELPWEHLENRAWEREWLQYF
                     APLRFGERFWVCPSGTESPDPDAIVMHLDPGLAFGTGTHQTTALCLEWLATHEIAGRD
                     VIDYGCGSGILGVAALLLGAASVRAVDIDPQALTATRDNAARNRIDPAQLVVGEPALL
                     ERAAPAGLLLANILAGPLIELAPRLAALVSPGGHAVLSGVLQTQAEAVRAAYLPWFTH
                     LATAQRDEWCRIELLRTERPAPCPR"
     gene            11376..12293
                     /locus_tag="PLW13_07920"
     CDS             11376..12293
                     /locus_tag="PLW13_07920"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HQY70338.1"
                     /translation="MPALITRCPRCQTAFRVTAAQLGAAAGLVRCGACDHVFEAERQQ
                     VSGEPGLNLPPGLVTPAPGPAGLDESYIRDLLREGPESAAPPAAPTDSDALAEGHGAA
                     SAAVAATVAALDAPAIDAAQPPGSPPVFVPPPIEIGAPARAAPVPRRIGWMLGCIAAA
                     LALLVQLAWHDRDRLVRDPDWRPRLDAACALLGCTLARPRDTALIRSDGLLIRPAPGR
                     EGVLLVDALLSNLAAYPQAWPQLEIVFSDMQGRALAGRVFMPSEYLPAWPRGDMPVRQ
                     AVAVHLEIRDPGASATNYRLFVRPAPATG"
     gene            12401..13405
                     /gene="dusB"
                     /locus_tag="PLW13_07925"
     CDS             12401..13405
                     /gene="dusB"
                     /locus_tag="PLW13_07925"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQY70339.1"
                     /translation="MLTFRERRSLLRIGSHCVDTRVVLAPMVGVTDHPFRHLCVRLGT
                     RLAIAEMVSADTRLWSAPGARRRLTADSHGGPRWVQIVGNEPAQMAEGARRQAARGAG
                     IIDINMGCPARNVCRKAAGSALLRDERLVGEILDAVVNAVPDVPVTLKMRTGWSPSDR
                     NGVRIARIAEDCGVRALSVHGRTRACRFEGSAEYDTIRDIVAAVRLPVIANGDIDSAA
                     KARAVLEHTGAAAVMIGRAAQGRPWLCGQIDAALATGAEPPQPARELLEQLMLGHLET
                     LHGFYGETVGARIARKHVGWYLAERAGARDFLRDFYRLDTAATQLAALGDYFSGARAA
                     "
     gene            13402..13710
                     /gene="fis"
                     /locus_tag="PLW13_07930"
     CDS             13402..13710
                     /gene="fis"
                     /locus_tag="PLW13_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HQY70340.1"
                     /translation="MNQINHAPPRYPEVHTGEAGGAPGQPEPRSMRQSVEAALDNYFA
                     QLDGQMVTDVYDMVLAEVEAPLLEAVLKYTRNNQTLAATVLGLNRGTLRKKLKRYGML
                     "
     gene            13713..>14973
                     /gene="purH"
                     /locus_tag="PLW13_07935"
     CDS             13713..>14973
                     /gene="purH"
                     /locus_tag="PLW13_07935"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQY70341.1"
                     /translation="MKDTGDTVAVRRALISVSDKTGVVEFARALAGLGVEILSTGGTQ
                     KLLAASGVPVREVSDYTGFPEMMDGRVKTLHPRVHGGILGRRGEDDAVMAAHGIAPID
                     LVAVNLYPFEQTVARPDCDLPTAIENIDIGGPTMVRSAAKNHRHVGIVVDAADYARVV
                     EELKTCGGTTAATRFDLAVKAFEHTARYDSAIANYLGALDPTGARGLFPRTFSLQFAK
                     AQEMRYGENPHQEAAFYVEHQAGEASISTARQLQGKELSYNNIADTDAALECVKQFHD
                     SPACVIVKHANPCGVALGATMREAYEQAWATDTESAFGGIIAFNRELDAETAALIVER
                     QFVEVIIAPAASADAVSAVAAKKNVRLLTCGALPETSAPRLDYKRVTGGLLVQDADFA
                     LFGELRVVSARAPSEDEMRDLLFAWKVA"
BASE COUNT         2261 a         5359 c         5110 g         2243 t
ORIGIN      
        1 gcaggacgcg gcgcggagct acagggacgt actcgtgcgt gccccgcagg gcaatggcgg
       61 gcagcacagc cagcgacgca ctgctgcagt ggttgagtca gcccgtcttc ccgcgccgcg
      121 cgcgctcgat gatcgcgttg aggcgctgct gcatctgcgc atcgggctgg tagctgagcc
      181 ccttgcgcgc gagctgctcg gccagcgcgt agtcgccgcg ctccagcctg agcgtcgcga
      241 gctcgcaata gactgccggg tcggccggcg cgatacgcag cgcgcgctcg gccgcggcga
      301 tcgcggcgtc gagattgccg ccacggcgct ccgtcgcggc ctgcgcgagc agggtgttca
      361 ccgcggtgtt ggcgctcggc ggcggcgcct gcagatcgat ctgcccggcg ccaccggcgc
      421 tgcccgctgg cccggcgaca gcgggcgcct cgatcgtgaa actgtcctcg gacgcgggcg
      481 cggtagcgcc cggaagcggc atcgtcgatg cgggtgccgg cgctcctgga tacgtggcag
      541 gcaacgcctg gccagtttgc ggcggttgcg cggccggata ggccgacgct tgcgcagcag
      601 tcgaggccgt aggcgcggga tagaccttca ccgcgctcgt ctgcggctgc gccgtggcgg
      661 gcgctcggtt ggcgggcgcc gcacgcccgt tacccggttc cggcacgtcg acgcgcggtg
      721 gcgtatgcgt cgagccggcg aactgcccgg cacaggcgcc gagcagaggc aggcaggcta
      781 gcgcgccgat gcgcagcacg cgcaagcaca ggccggtcga ttcgctcatc tttgcttccc
      841 cgtctgcttg ccgctgttgc atgtccgtcg cagcatgcga tggcgcgagg ctgtcgcctc
      901 agcgatcccc gccaaaccag cgcttccacc accggtcacg cgctttttcc tcctgctccg
      961 gcgcgctgtc gggttgcgcc gccgcgcccg gctcggccgc ggcaccgggc tccgcggccg
     1021 gcttgccgcc ggaccacggc cagcgcagcc ccgagccggt gcaggcagcc tcgtaatcgg
     1081 gctcggtgcc gttcatgaac ggcatcagcc gcgcaccggg gcagccttcc ccgctcaacc
     1141 cgccgcccgc ttcgtcgacc cacgtttcgg tgatgccccc gggcgcccgt gagcgacgcg
     1201 gttcgcggct caccgccgcc atcagcccgg tccacgcggg caatgcgccg ctggcacccg
     1261 tgagtgcggt ggtgccgttg tcgtcgcgcc cgatccacac cacggcgagc agatcacccg
     1321 agtaacccgc gaaccagctg tcgcgctggt cgtcggtggt accggtcttg ccggccacca
     1381 ccagccccgc cggcaggcgg ttgtaggccg agcgcgcggt acccgcgctc accacttcct
     1441 tcaatgcgta ctccagcagg tacaccgccg actggcggac cttctgctcc accgccacag
     1501 gatagcgcgt cagcggctcg cccgaggcat ccatgacctc gcggattgcg ctcagcggcg
     1561 aactgaagcc gcccgaagcg atggtctggt acatcccggc gacctcgatc gggctgagcg
     1621 ccacggcgcc gagcgcgacc gatggcagcg ccggcacctc gcgatcgacg ccgaggcggt
     1681 ggatcgtatc gaccacgcgc tcgatgccca gctccagccc gagccgcgcc gtggactggt
     1741 tgtacgactg tgccagcgca cggtgcagcc gcaccatgcc gtgatcctcg cgatcgtagt
     1801 tgcgcggacg ccacacctgg ccgttggcgc ccttgtgcgc caccgggccg tcgtcgagcg
     1861 gcgacagcaa ggtgtagcgt ccgtcctcga gcgccgtgag gtagaccgcg ggcttcatca
     1921 gcgagcccac ggggcgcaac gcatcgagcg cgcggttgaa gccggccacg cgcgagttgc
     1981 gcccgcccac gaccgccagc acctcgccgc tgtcgacgcg ggtcacgatc accgcaccct
     2041 cgagcccctg ctgcggcttg ccgcgcgcct tgaagtcgct ctcgatcttc gccagcgtct
     2101 cctgcagcga ggcttcggcc gcgcgctgca ccgcgggatc caggccagtg aatatctgca
     2161 ggccttcgga gctgaggacc tcctcgctgt agtcgcgccg cagctggcgc cgcaccaggt
     2221 cgaggtacgc cgggtagaac ttcgagcgcg cctgctcgcg atcgcccaca ccgagcggca
     2281 tctgcgcgat ctgtgccgct tgcgcggcgt cgatgacctg ctgatcgacc atcaatgcga
     2341 gcaccgtgtc gcgccgctcg cggctgcgct cgggatgacg ccacgggtcg taatacgagg
     2401 ggccgcgcac catgccgacc agcagcgcga tctgcggcgc gccgagttcc gcgatgggcc
     2461 ggttgaagta gtagtggctc gcgagcccga aaccgtggat cgcgcggtgc ccgtcctgcc
     2521 cgaggtagat ctcgttcagg taggcctcga ggatctcctc cttgctgtag cgcagttcca
     2581 tcaccagtgc cagcgcgagc tccatcagct tgcgcgtgat ggtgcgctcg cgggtgagga
     2641 agaaattctt caccagctgc tgcgtcagcg tgctgccgcc ctgctccagc gcgccgctgc
     2701 tcacgttgac ccacatcgcg cgcgcgatgc ccttcggcga gacgccgtgg tgcgagtcga
     2761 agttctggtc ctcgaccgcc atcagcgtgc ggatcagcaa cggcggcaca tccgacaccc
     2821 gcaccaggat gcggtcctcg ttgtgcgccg gatagatccc gccgatgtgc acgggctcga
     2881 gccgcgcgag cgccacttcg gcgccctgct cgtcgtggac cgcctgcacc gagttgccgg
     2941 cgaacgcgac gcgcaggcgc cgtgccggct cctcgccgtc ccagaaccgg aagccgcgcg
     3001 tcgcgatcga caccgtgctg cccgagacgc tgtaggttcc ggcgctgccc gcgccgccgg
     3061 cggcacgata acccagcgtt tccagctccg cgcccagcgc gcgcggcccc accggtgcgc
     3121 cggcgtagag ctctagcgga cggccgtaga ccatcgcggg caccgcccac ttcttgcccg
     3181 agaattcacg ctggatgtag gcatccaggt acatgaatgc gagcacgccc accaccgcgg
     3241 cggcggccag caggcgcagc agcaccacgg gccagcgaag cggtcgggct gttctcgggg
     3301 gtttgcgcgc gggcttgcgt gcggctctgc gcggcggctt gcgttcgggt ggctggacga
     3361 cgccgcgttg cgggctgcgc tcggatggtg aaggcatggc gagaaagtgt acggagaagg
     3421 ccctagccct acaaataaat gcaggttttc acattgagcc gcgcccgcgg gctgcggata
     3481 atcgcgcgcc aaccggcatt cggcccgggg cacaacggaa cagagcatga agaagaagta
     3541 caacgtctat ggcgtgggcg ccgcgctggt cgacaccgag atcgaggtcg atgacgcatt
     3601 cctcggtagc gccggcatcg acaagggcgt gatgacgctg gtcgacgagc cgcgccagga
     3661 tcgcctgctc gagctgctga gcacgcacct ggtggcctcg cgacgcgcaa gcggcggatc
     3721 ggccgcgaac tcggtgatcg ccacgagctg cttcggcggc cgcgccttct acagctgcaa
     3781 ggtggccgac gacgagaacg ggcgcttcta cctggccgac atggcgcgcg ccggtgtcga
     3841 ctgcaacgtt tgcgcgggac gcgagagcgg cgtgaccggc aagtgcctgg tgatgatcac
     3901 gccggacgcc gagcgcacga tgaacacgtt tctcggcatc agctcgacgc tgtcggcaca
     3961 gaacctcgac gcagcggcgc tcgcggcctc ggaattcgtc tacgtcgagg gctacctggt
     4021 cacctcggac agcggacgcg cggcggcgat cgcggcgcgc gaactggccg agcgcagcgg
     4081 cgtgcgcacc gcgctcagct tctcggatcc gggcatcgtc gcgcactttc gcgacggcct
     4141 ggcgcagatg ctgggcaacg gcgtcgacct gctgttctgc aatctcgccg aagcgcagga
     4201 atggaccggc gagcagcgcc tcgaggacgc ggccgaggga ctgcgcaagg tggcgcgatc
     4261 cttcgcgatc accctgggcg gcgagggcgc gctggtcttc gacggccgcc attaccaccg
     4321 catcgcgccg tacccggtga agcccatcga caccaacggc gcaggcgaca tgttcgccgg
     4381 cgcctacatc tacggcatca ccaacggctt cgacgcggtc gcggcggggc ggctcgcgaa
     4441 ccgcgccgcg gccgaactgg tcacgcacta cggcccgcgc ctgcccgccg cggcgcaccg
     4501 gcggctgctg gacgaattcg accgcgagca gggatgacaa tgtcgccatg aatggcgacc
     4561 cacgggtctc cggataaatc cggacccacg gacgcgaccc tcagccctgc gccagcaatt
     4621 cgcgcaggtc gatcacgccg gcgttggcgc gcgagatgta cgaggccatc accagcgagt
     4681 ggttcgccag cggcccgtaa ccggtgccgt tcaggatcac cggactccac atcgggtcct
     4741 gcgtcccctc gagctcgcgg atgatctggc gcaggctgac aagcgcattc ttgcgttcga
     4801 gcacgtcggc gaaatcgacc tcgatcgcct tcagtatctg gatcagcgcc caggtcgagc
     4861 cgcgcgcctc gtagaacacg tcgtcgatct ccagccacgg cgtcttcgat tcgaactcgc
     4921 tcgccccggg cgtcgactgg tgcgcggccg gatcaccggc cagatcggtg ttcaggcgcg
     4981 ccttgccgac actggcgctc aggcgctggg aaagactgcc gagccggctc tgcacagtgc
     5041 cgagccagta gcgcaggttg tcggcgcggg cgtagaactg cgcgtcgctg gcgtcggcat
     5101 cggcgaggcg cttggcgtag gactcgaggt gcctgatgcc ctcgcggtat tccgactcgg
     5161 tgggcggcag caaccaggaa tcgttgtcga aattgaactg cggctctgcc agcgcaagat
     5221 ccgcgtcctc cctggactgc gattgcgatc ggctcagcgt ctcgcgcatc gccttggcga
     5281 ggtcgcgcac ctgcaccagc gcgccgaact cccagttcgg catgttgtcg agcagcacgc
     5341 cgggcggcag ccggtcgttg ctgaggtaac cgccgggctt gtccagcagc gtctcggcca
     5401 cgcctaccag ggtggccgtg gtcgcggtgc ccaccaccac cggccgcccc tggcgctcgg
     5461 cgaagcgcgc agcgttgtcg cgcacgtcaa agggctgcgg ctcgttcgac cagtacaggc
     5521 ccagtcccac ggtcatgatc gccagcgcgc cgaccaccgc ggccgtggcc accgcggcgg
     5581 cgcctcccgg cgccagcgcc ttgcccaacg cccgggtgcg ccctgcatcg atgttcacgt
     5641 ccgtatccat gtactactcc ccgattttcc tgacgcgcgc ctcggcgcac tgctcgacgc
     5701 catcctccag ccgcaggcag aacggcagca gcgcaccggg ctgcagcgcg ctgcccatgc
     5761 cgcccaccat caggtgatag ccgccgggct gcaggctcag gtccgcgccc ggggcgatct
     5821 cgagcctggg caccgggtac atgcgcatca tgccgtcggc atggctgtgg ccgtggatgg
     5881 ccgcgctggc cgcgccttcc acgcgcgcgc ccaccaccgc gagcggcgcc gtgccgccgt
     5941 tgtgcagctc catgtagatg gccgcgccgg tctgtccggg cggcggctgg cgcagccagg
     6001 cacccgtgat atccagcgcg gcggcatatg ccgggccgac gcccgcgagt gccgcgagcg
     6061 ccgcgagcgc cgcgagcagc agtacggccc gttggggcaa cgaatatgtc atgggcaaga
     6121 gcgcctcgat ccgagtctgc ggtcattgtg ccacagggcc cggcggcgca ccggcagtaa
     6181 ttattcgacc tcggcagacg gcgagggcaa tggctagaat gcgcctcgcg gccgactccg
     6241 gccgccgccg gacggatgca cgtatgctga gactgctgct gatcgccgcc ctgctgtggg
     6301 gcgccaacat tgccactgcg aaccaggatg ccttgccggg cgcctcgcgc gacgccggga
     6361 tgctgggtgg ctccgcgcac gccttcctgc gcgtcgacga agcctacgca ctgcgggctg
     6421 aactgcgcga cgctcaggtg ctggtgcagt gggacatcgc tccgggctac tacctgtaca
     6481 aggaacggat cggtttcaag tggaagggca aggacgccgc cgaggcgatc gtggtcacgc
     6541 tgcccgcggg cgaggcgcac gaggacgcct atctcggggc aaccgaggtc taccgcggca
     6601 cgctcgcggt gaccctgccg ttgcccggct ctgagcccgc gctgctcgag gtgaagtcgc
     6661 agggctgcgc cgacgccgga ctgtgctacc cgccgcgcac acgggtgttt gccgtcgatc
     6721 ctgtcacggg cagcctggac gagaccgaac cggagcagat cccggtcacc gccgcgcaaa
     6781 gcgcggcgcc ggcacccgct accggcccgg cggacctcgc gaagctgcta tggatggcgc
     6841 tgctggccgc gctcggcggc atgatcctga acctgatgcc ctgcgtgttc ccggtgctgt
     6901 cattgaaggt gctgggcatc gcggccaagg gcggcaacgg tcacggcaag gcgctgcacg
     6961 gactggccta tgccgccggc gtggtcctga gcttcgtcgc cgtcgcgggc ctgctgctcg
     7021 cgctgcgcgg cgcgggtgcg gcgatcggct ggggtttcca cctgcagtcg ccggcattcg
     7081 tcgcggccct ggcctacctg ttcgtggtca tgggactggc gctctcgggg atgatcaacc
     7141 tgggctcggg cgtgatgggc agcggcgagg ccctcgcgcg ccagggcggc taccgcggca
     7201 cgtttttcac cggcgtgctc gcgaccgtgg tcgccagccc ctgttcggcg ccgttcatgg
     7261 gcaccgcgct cggcttcgcg atgacgcaac cgcccgttta cgcgctggcg atcttcgccg
     7321 cgctcgggct cggcatggcc gcgccgttcg tgctgctcac cctgagcccc gcgctgctgc
     7381 ggcgcctgcc ccaccccggc ccctggatgg acggcttcaa gcagttcctc gcctttccgc
     7441 tctacgccgc cgcggtctgg ctgctgtggg tgctgggcaa ccagaccggc gtgaacggca
     7501 tggcggcggt gaccggcggc tgcgtgctcc tggccctcgg actgtggtgc tgggaacacg
     7561 cgaagctgca ccgccgtccg ctccccctgc ggctgctggg cgcgctgatc tgcgtgctcg
     7621 cgctgctcgt gctgcgctca ccgttgctcg acgagcgccc ggccgtgcgc accgacagca
     7681 ccggcgcctg ggaggactac agcccggccc ggctggagga gctgcgcgct gccggcacgc
     7741 cggtcttcgt caacgtcacc gccgactggt gcatcacctg cctggcgaac gagagaatcg
     7801 cattggcctc ggaagaggtg ctggcggcgt ttcgcgaccg cggcatcgtg tatctcaagg
     7861 gtgactggac caacagcgac ccggagctca cggccctgtt gcaggcgcac gggcgcagcg
     7921 gcgtgccgct gtacctgttc taccccgccg cggccggggc ccaggcaact ctgctgcccc
     7981 aattgctgac accatcgatc gtgctggagt tcatcaacac gcgctgatcc tgctccattt
     8041 ttggcgaacc tgcccgcatc gtgcctgaac tgatccgttc tatggacgcc acgggtgaat
     8101 tgcggcagaa tcgcgccagc ttcccgacaa ccccccggca gcgctatgcg ccgcgatccg
     8161 caagcagaaa ccgacatggc cacgatcctg gtgctgcacg gaccaaacct gaacctgctg
     8221 ggcacccggg agccggaaac ctacggcagc gtgacgctgg agcagatcaa ccagcgtctc
     8281 gccggcatcg ccgtcggcgc gggccatcac ctgcagacgc tgcagagcaa tgccgaacac
     8341 gagctgatcg agcgcatcca tgccgcccgc tccgagggca tcggcttcat cgtgatcaac
     8401 ccgggcggct tcacgcatac cagtgtcgca ctgcgcgatg ccctgctcgg ggtcgccgtc
     8461 ccgttcatcg aagtacatat ttccaacgtc catgcgcgcg agccgttccg ccgccactcg
     8521 tatttctccg acgtcgccgt cggggtgatc tgcggactcg gtccgatcgg atacgaactg
     8581 gcgctgcagg cggcaatcgc gcgcctcaca ccagcaacct gaaaccagcg aagggcacgc
     8641 catggatatc cgcaagatca agaccctcat cgagctgctc gaaggctccg atgtcaccga
     8701 aatcgaaatc tgcgagggcg aggaatccgt gcgcatcagc cgcggccatg cgcagccggt
     8761 gtacgccgcg ccgccgatgg cgatgccgtt cacggcggcc caccccgcgc cggcggctcc
     8821 cgcggcgcca gcgccgatgg cgcccgcggc tgccgccgaa ccggccgggc acctggtgcg
     8881 ctcgccgatg gtcggcacct tctaccgctc gccctcgccc ggcgccaagg ccttcgtcga
     8941 ggtcgggcag agcgtgaagg ccggcgaggt gatctgcatc atcgaggcca tgaaaatgat
     9001 gaaccacatc gagtccgaca aggccggcac gatcagcgcg atcctggtcg aggacggcca
     9061 gccggtcgag ttcgaccagc cgctgttcag tatcgtctga cgatcggcca ccgccccagc
     9121 acaggatcgc catttatgct cgagaaagtt gtcattgcga accggggcga gatcgcgctg
     9181 cgcatcctgc gcgcctgccg ggaactcggc atccggaccg tcgcggtgca ctcgcaggtc
     9241 gatcgcgacc tgatgcacgt acgcctcgcc gacgaggccg tgtgcatcgg ccccaacccc
     9301 tcggggcaga gttacctcaa cgtgccctcg atcatcagcg ccgccgagat caccgatgcc
     9361 gtcgccatcc accccggcta cggcttcctg gccgagaacg cggacttcgc cgaacaggtc
     9421 gagaagagcg gcttcgtctt catcggcccc actgccgacg tgatccgcct gatgggcgac
     9481 aaggtgagcg ccaaggacgc catgaaggcc gccggcgtgc ccacggtgcc gggctccgac
     9541 ggcgcgctgc ccgaggacgg cgacgagatc ctcgccatgg cgcgcgcagt gggctatccc
     9601 gtcatcatca aggccgcggc cggcggcggc ggtcgcggca tgcgcgtggt gcacagcgag
     9661 gcggcgctgc tgaactcggt gttcgtgacc cgcaacgagg cgcaggcagc cttcggcgac
     9721 ggcacggtct acctggagaa gttcctcgag aacccgcgcc acgtcgagtt ccaggtgctt
     9781 tgcgacggca agggcggcgc gatccacctc ggcgaccgcg actgctcgct gcagcgccgc
     9841 caccagaagg tgctggaaga ggcgcccgcg cccggcatcc ccgacgcgct caagcgcgcg
     9901 atagccgagc gctgcgtgca ggcctgcctg aagatcgggt accgcggcgc cggcaccttc
     9961 gagttcctct acgagaacgg ccagttcttc ttcatcgaga tgaatacccg cgtgcaggtc
    10021 gagcatcccg tgaccgaggc catcaccggc atcgacatcg tgcgcgaaca gctgctgatc
    10081 gccggtggcc agccgctgtc gatccgccag gaggacgtgc attttcgcgg ccacgccttc
    10141 gagtgccgga tcaacgccga ggatccgaag accttcgcgc ccagccccgg caaggtgcgg
    10201 ctgttccacg cgcccggcgg caacggcatc cgcgtcgact cgcacctcta cagcggctac
    10261 agcgtgccgc cgaattacga ctcgctgatc ggcaaggtga tcagctgggc cgaggaccgc
    10321 cacggcgccc tgatgcgcat gcgcaacgcg ctcgacgaga tcgtggtcga gggcatccgc
    10381 accaatattc cgctgcacca gcagctggtg cgcgacgagg cgttccatcg cggtggcgtg
    10441 agcattcact acctagaaaa gaagcttcag aaataactcc cgatgggctg gctgcagctg
    10501 cgcctggtag cgaccgccga ccgcgtcgag gtggtggaag acgcgttgct cgctctcggt
    10561 gcgctgtcgg tcacgctcgc tgaccaccgc gacgtgccga tcctcgagcc cggggtcggc
    10621 gagacgccgc tgtggcccga agtcgacctg gtcgcgctgt tcgacaagga caccgacacc
    10681 gctgccgtcg gcgcggcact cgccgaactg ctgggcgagg cagcgcgcga attgccgtgg
    10741 gaacacctcg agaaccgcgc ctgggagcgc gagtggctgc agtatttcgc gccgctgcgc
    10801 tttggcgagc gcttctgggt ctgcccgagc ggcacggaat ctcccgaccc ggacgccatc
    10861 gtgatgcacc tggatccggg gctcgccttc ggcaccggca cgcaccagac cacggcgctc
    10921 tgcctggaat ggctcgcgac gcacgagatc gccggccgcg acgtgatcga ttacggctgc
    10981 ggctccggca tcctcggcgt ggcggcgctg ctgctgggcg ccgcgagcgt gcgcgcggtc
    11041 gacatcgacc cccaggcgct caccgctacc cgtgacaacg cggcgcgcaa ccgcatcgat
    11101 ccggcgcaac tggtcgtggg tgaacccgcg ctgctcgaac gcgccgcgcc cgcggggctg
    11161 ctgctcgcca acatactcgc cgggccgctg atcgagctcg cgccgcgcct ggcggcactg
    11221 gtctcgcccg gcggacacgc cgtgttgtcg ggcgtgctgc agacccaggc cgaggcggtg
    11281 cgcgcggcct acctgccgtg gttcacgcat ctcgccaccg cgcagcgcga cgaatggtgc
    11341 cgcatcgaac tgctgcgcac ggagcgcccg gccccgtgcc cgcgctgatc acccgctgtc
    11401 cgcggtgcca gaccgcgttc cgcgtcaccg cggcgcagct gggcgcggcc gcagggctgg
    11461 tacgttgcgg cgcctgcgat cacgtgttcg aagccgaacg ccagcaggtg tcgggcgaac
    11521 ccggactgaa cctgccaccc ggcctcgtga cgccagcacc cggccccgcc ggtctggacg
    11581 agagctacat ccgcgacctg ctgcgcgagg ggccggaatc cgccgcgccg cccgccgcac
    11641 cgaccgacag cgatgcgctc gccgagggcc atggcgcggc cagcgccgca gtggcggcaa
    11701 cggtcgccgc cctcgacgcg ccagcgatcg acgcggcgca gccgcccggc tcaccaccgg
    11761 tattcgttcc gccgccgata gagatcggag ccccggccag ggcggcgccg gtaccgcggc
    11821 gcatcggctg gatgctgggc tgcatcgccg ccgcgctggc gctgctcgtg cagttggcct
    11881 ggcacgaccg cgaccggctc gtgcgcgatc ccgactggcg gccgcgactg gatgcggcct
    11941 gcgcgctgct cggctgcacg ctggcgcgcc cgcgcgacac ggcgctgatc cgcagcgacg
    12001 gactcctgat caggccggcc cccggacgcg agggcgtgct gctggtcgat gcgctgctca
    12061 gcaacctcgc cgcgtatccg caagcctggc cgcagctgga aatcgtgttc tccgacatgc
    12121 aggggcgcgc gcttgccggc cgcgtgttca tgccgtcaga gtacctgccg gcgtggccgc
    12181 gcggcgacat gccggtacgc caggccgtcg cggtgcacct ggagatccgc gaccccggcg
    12241 ccagcgccac caactaccgc ctgttcgtgc gccccgcgcc tgccaccggc tgagcacgga
    12301 ccgcagcgcg ccgcgacgcc acaatgccgg tgcaattccc tctccccctt taatcacccg
    12361 cagcctgccg ctatcattcg ccccccctgt ggggaatccc gtgctcactt tccgcgagag
    12421 gcgttcattg ctgcgcatcg gttctcattg cgtcgacact cgcgtggtgc tcgcgccgat
    12481 ggtcggcgtg acggaccatc cgttccggca cctctgcgtg cgcctcggca cgcggctcgc
    12541 catcgccgag atggtttccg ccgacacgcg cctgtggagc gcaccgggcg cgcggcgacg
    12601 cctcacggct gacagtcacg gcggaccgcg ctgggtacag atcgtgggca acgagccggc
    12661 gcagatggcc gagggcgcgc gccgccaggc ggcgcgcggc gccgggatca tcgacatcaa
    12721 catgggttgc ccggccagga acgtctgccg caaggcggca ggctccgcgc tgctgcgtga
    12781 cgagcggctg gtaggcgaga tcctcgacgc cgtggtgaac gcggtgcccg acgtaccggt
    12841 cacgctgaag atgcgcaccg gctggagccc gtccgatcgc aacggtgtgc gcatcgcacg
    12901 cattgccgag gactgtggcg tgcgcgcgct cagcgtgcac ggacgcacgc gcgcctgtcg
    12961 cttcgagggc agcgccgagt acgacacgat ccgcgacatc gtcgctgcgg tgcgcctgcc
    13021 ggtgatcgcc aacggcgata tcgacagtgc cgcgaaagcc cgcgcggtgc tcgaacatac
    13081 cggcgcggcc gcggtgatga tcggtcgcgc ggcgcagggc cgtccatggc tctgcggaca
    13141 gatcgacgcg gcgctggcca ccggcgccga gccgccacaa cctgcgcgcg agctcctcga
    13201 gcaactgatg ctcgggcacc tggagacgct gcacggcttc tacggtgaaa cggtgggggc
    13261 gcgcatcgcg cgcaagcacg tcggctggta ccttgccgag cgtgccgggg cgcgcgactt
    13321 cctgcgcgat ttctaccgcc tcgacaccgc ggcgacgcag ctggccgcgc tgggcgacta
    13381 tttctcgggg gccagggcag catgaaccag atcaaccacg cgccgccccg ctaccccgag
    13441 gtccataccg gcgaggccgg cggcgcgccg ggccaacccg agccgcgctc gatgcgccag
    13501 agcgtcgagg ctgcgctgga caattacttc gcgcagctcg acgggcagat ggtcaccgat
    13561 gtctacgaca tggtgctggc cgaagtcgag gcgccgctgc tcgaggcggt gctgaagtac
    13621 acgcgcaaca accagacgct cgccgcgacc gtgctcggtc tcaaccgcgg cacgctgcgc
    13681 aagaaactca aacgctacgg gatgctgtga acatgaaaga taccggggat accgttgcgg
    13741 tgcgccgcgc cctgatcagc gtgtcggaca agaccggcgt ggtggagttc gcgcgcgcgc
    13801 tcgccgggct cggcgtggag atactctcga ccggcggcac ccaaaagctg ctcgcggcga
    13861 gcggcgtgcc cgtgcgcgag gtgtcggatt acaccggctt tcccgagatg atggacggcc
    13921 gcgtcaagac gcttcacccg cgcgtgcacg gcggcatcct cgggcggcgc ggcgaggacg
    13981 acgcggtgat ggcggcgcac ggcatcgcgc cgatcgatct ggtggcggtg aacctctacc
    14041 ccttcgagca gacggtggcc cgccccgact gcgacctgcc gaccgcgatc gagaacatcg
    14101 atatcggcgg cccgacgatg gtgcgctcgg ccgcgaagaa ccatcgtcat gtcggcatcg
    14161 tggtcgatgc cgccgactac gcgcgcgtcg tcgaggagct gaagacttgc ggcggcacca
    14221 cggccgcgac gcgcttcgac cttgcggtga aggccttcga gcacaccgcg cgctacgaca
    14281 gcgcgatcgc caactacctc ggcgcgctgg acccgaccgg tgcgcgcggc ctgtttccgc
    14341 gcacgttcag cctgcagttc gccaaggcgc aggagatgcg ctacggcgag aacccgcacc
    14401 aggaggcggc cttctacgtc gagcaccagg cgggcgaggc gagcatcagc accgcgcgcc
    14461 agctgcaggg caaggaactc tcgtacaaca acatcgccga caccgacgcg gcactggagt
    14521 gcgtgaagca gttccacgac agcccggcgt gcgtgatcgt gaaacacgcc aacccctgcg
    14581 gtgtcgcgct cggcgccacg atgcgcgagg cctacgaaca ggcgtgggcc accgacacgg
    14641 aatccgcctt cggcggcatc atcgccttca accgcgagtt ggacgcagag accgccgcgc
    14701 tgatcgtcga gcgccagttc gtcgaggtga tcatcgcgcc ggcggcgagc gcagacgcgg
    14761 tgagcgcggt ggccgcgaag aagaacgtgc gcctgctgac ctgcggcgcg ctacccgaaa
    14821 cgtccgcgcc gcgcctcgat tacaaacgcg tcaccggtgg cctgctggtg caggacgccg
    14881 actttgcgct gttcggcgag ctgcgcgtcg tgagcgcgcg cgcgccgtcg gaagacgaga
    14941 tgcgcgacct gctgttcgcg tggaaggtgg cga
//
LOCUS       DAORUQ010000070        14795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3867_length_14795_cov_6.218318, whole genome shotgun sequence.
ACCESSION   DAORUQ010000070 DAORUQ010000000
VERSION     DAORUQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14795
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_14795_cov_6.218318"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..335)
                     /locus_tag="PLW13_07940"
     CDS             complement(12..335)
                     /locus_tag="PLW13_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HQY70342.1"
                     /translation="MNGRIRVAALLIDLEAALRSRLLWDAGEPSPEALASTQPFCIDT
                     LDFPQWLQFVFLPRMRALLDADLPLPAQCAVAEMAEMHFAAADAADVIALLREIDLAV
                     VAASP"
     gene            complement(337..2439)
                     /gene="dinG"
                     /locus_tag="PLW13_07945"
     CDS             complement(337..2439)
                     /gene="dinG"
                     /locus_tag="PLW13_07945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HQY70343.1"
                     /translation="MLNDDVKNAIQGAYRNWLNARGLRPRSGQRAMIAQVANALARSN
                     DPDNEASPICVVEAGTGTGKTIAYAVAAIPIAQALGKKLVISTATVALQEQLFHRDLP
                     DLKTAGALDFSYALAKGRGRYVCPARLDSLLRGARDEDGAQLALVEQIVPSVNQETRE
                     LVRELYTAVTDGSWAGDRDSWPAALPDSRWALMTTDHHQCSGRRCSYVSQCPFFTARE
                     GLDGVDCIVANHDLVLADLALGGGAVLPAPEDAIYVFDEAHHLPDKACNHLSFRARLG
                     LAREHAEDCRRLATELMAEPRSSDALRRRLAEVPALCEELDHGLSAAGRLLGALLAGA
                     GRKGDTAVEHRLPHGVVPADVAAIAATLHHALRKLSDALEGAEDELSRQLDTGGAAAQ
                     DRYAAMGLSIGRVQRLAGLWKSWAEPDPAGAAPQARWIRASEQQSSADLEVCAAPVLA
                     ANVLRDSIWTRAAGAVLTSATLTALGKFDRIVLRAGLPADCVFATVPSPFDYRGRACL
                     AVPRLAADPGDPVAHTREVADWLDANLDRAEASLVLFASRKQMREVYDRVSLGLRDRI
                     LLQDHFAKQELLRLHRERVDVGEGSVIFGLASFAEGIDLPGGYCMHVVIAKLPFAVPD
                     DPVEAALAEWVESRGGNPFMEISVPDAALRLVQASGRLLRTEEDTGRITLLDRRVVTR
                     QYGRRILDSLPPFRREVA"
     gene            2540..3475
                     /locus_tag="PLW13_07950"
     CDS             2540..3475
                     /locus_tag="PLW13_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY70344.1"
                     /translation="MIETLSHGITCIDTGYHRAGLAACYLVVDDGEAALIETGTALNL
                     PAILRALTDCGVAPEQLRYIMPTHVHLDHAGGAGALLARCPQATLVAHPRGARHLVDP
                     ERLIAGAKAVYGEESFTRMYGDIVPAPLERVREAADGSRWTLGRRELLVRDTPGHARH
                     HFCVWDETSRGWFTGDTFGIAYREFYCGGEPYLIPTTTPVQFEPEALLASIELLMAAE
                     PHYCYLTHFGRIAASAHHAGSLRRQVLDYVRLAEGIDITDPHAQAALTEALTTYTLEG
                     LHAAGSTLPVTTLRALLALDMELSAQGLVHWMRHS"
     gene            complement(3532..4674)
                     /locus_tag="PLW13_07955"
     CDS             complement(3532..4674)
                     /locus_tag="PLW13_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQY70345.1"
                     /translation="MSQDRFAQIRPYNDDEVRGVLDRLLRNAEFIAALTRLRFPRAGG
                     ALGALLRPIVRLYLGRQLAGVASVAGFQKVVEKYMTAMIESSTTAFTVSGLERLDPAR
                     ACLFIGNHRDIALDPAFVNYALYANGCDTVRIAIGDNLLTKDYVSDLMRLNKSFIVRR
                     SAKGPRQMLAALQELSGYIRHSLLEECSSIWIAQREGRAKDGWDRTEPAIIKMLCISK
                     ARETPIAAHLRALNIVPVSISYEWDPCDGAKARELYLRASEGDYEKEEHEDVASIAHS
                     ITSPKGAVHVSFGEPLDADFAGPDEVAAEIDRQVLALYRVHSSNIAAFRMLQGRLPEG
                     FADDAAVAAADAELRARLEALPAPHRPYLLAMYANPLLNRAALDAP"
     gene            4771..5532
                     /locus_tag="PLW13_07960"
     CDS             4771..5532
                     /locus_tag="PLW13_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY70346.1"
                     /translation="MSDDPILFSRDGHIARITLNNPAQHNALGAAEIGRFRDCLDAVE
                     TDPSLRVLLLTGNGERTFCAGASLKQFRSGDMSGELFSTLTDRLAALPIPSVCALNGN
                     VFGGGAEIALCCDYRIGVDDLRLLVPAARIGICYPLNGLQRYVDKLGLGAAKRILVAS
                     EELAAETLLAIGFLHRVVARERLDAESDAFARHLAGLAPLAVRAMKQILGGIARGDLD
                     SAGAARLVQQCTQSRDLQEGLAAQREKRAPRFTGE"
     gene            complement(5519..6562)
                     /locus_tag="PLW13_07965"
     CDS             complement(5519..6562)
                     /locus_tag="PLW13_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY70347.1"
                     /translation="MHRLRVPRHDLPAPIGAIMVEVAADFGITPSQVLEGTSIDEGAL
                     GAPDTRISLRDQTRMLENCLRRSGDPGFALHFGSRISVAALGELGYALMCCRNVQELM
                     EVMGHYHRLISGSFRIEVESGRDAVSIRLVGGMLHEAAEPADCEIFFAAAAASLRRLC
                     ALSDDALSATLAYPDPGYGEEYRRLICRDVRFDAPDSRLAVDRRYLDVPLQFANPTLL
                     KLYRQQCDELLAALNRSDGFTTEIRNYLLATPGRFPPFEEAADHLHVSPRTLRRRLDE
                     EGTNYQRIVHELRRQLAETYLRDSMLSIAEIADMLGYNDVSNFRRAFLSWTGRSPAAY
                     RRGMRGGAMLIPR"
     gene            6694..7785
                     /locus_tag="PLW13_07970"
     CDS             6694..7785
                     /locus_tag="PLW13_07970"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQY70348.1"
                     /translation="MGVQFVIDTPSEVRYFDQEAQRMDAGTAQQVAEIFQTPLTGSYN
                     WDYRVQDDRIKKLYELGKQLNWNVETDIDWGKRRTRLTPEQRDAIVAFKGYGPYEALS
                     EEQKQEFAEHENVWTLSQFLHGEQGALLVASQLTSCAPTLNAKLYAASQTFDEARHVE
                     VFNKYLQTKAGYMYPVDPALKSLLDKILTDPRWDLKFIGMQLIIEGLALSAFTTMKAT
                     TPKGELLNDVVELVLRDEARHVTFGINYLEEHLRNLSEAEREERARFAYEACVVARER
                     MIPKDVFHHWGWNVEEARRIYLEGEFRQMFRNLMFMRVMPNLRRIGLLTDSVRPLFEQ
                     LGMLDYENLKDDGDIDWAELSRPLFDTAA"
     gene            complement(7790..8572)
                     /locus_tag="PLW13_07975"
     CDS             complement(7790..8572)
                     /locus_tag="PLW13_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY70349.1"
                     /translation="MQDLSVTIFQQDLAWQDAPANRAQFARLLPAAAGSALALLPEMF
                     ATGFTMEPAACAEGMDGPTLGWLREQAALHGCAIAGSLAIEEGGAFRNRFLFARPDGG
                     LVHYDKRHLFRMAGEHHGYVAGSERRVIEWQGWRLCPQVCYDLRFPVWSRNRGDYDAL
                     LYVANWPARRREHWRALLVARAIENQAWVIGVNRTGVDGKGVDYAGDSLVIDPWGRVV
                     LDAGCAAGVHRVTLAAATIADCREKFPVHLDADAFRIGGAPQ"
     gene            complement(8560..9702)
                     /locus_tag="PLW13_07980"
     CDS             complement(8560..9702)
                     /locus_tag="PLW13_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQY70350.1"
                     /translation="MPQSKLPDVGTTIFTVMSRKAEAHGAINLSQGFPDFALPAGLGE
                     ALARHVAEGRNQYAPMAGVLALREQIALKLGRCYGCRVDPEAEVTVVPGATEAIFCAI
                     MATVRAGDEVILFDPSYDSYEPSVRLAGGRAVRIPLLPPAFAIDWQRVAEALTPRTRM
                     IVINSPHNPTGSVLGAADLDRLEELVQEHGLLVLSDEVYEHLVYDGAAHHSVLARPAL
                     AERAFAVFSFGKTYHATGWKTGYCVAPPELTREFRRVHQFVTFVAVTPIQHALADFMA
                     GAPAHVDRLPAFYQAKRDLLRRLLAGSRWRALPCAGTYFQLLDYSGIGDADDVAMAEE
                     LVSRHGVAAIPVSVFYERPPRLRLLRFCFAKQDETLVRAAELLCRI"
     gene            9946..>10436
                     /locus_tag="PLW13_07985"
     CDS             9946..>10436
                     /locus_tag="PLW13_07985"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQY70351.1"
                     /translation="MIHARHCAALLLGLAGSGMACANAEPPSAPVEDWAARGAVALAE
                     SRALFPGMPERARNVILFVGDGMGITTITAARILEGQQKGGSGEENSLAFERLPFVAL
                     SKTYSANQQTPDSAPTMSAMMTGNKTSDGLISIAATVAHGEPAREAVAAAAQPTLLEL
                     AEGA"
     assembly_gap    10437..10536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10537..11363
                     /locus_tag="PLW13_07990"
     CDS             <10537..11363
                     /locus_tag="PLW13_07990"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQY70352.1"
                     /translation="GTRDPEDHGARGVRRDGRDLTREWKRKFGGQYVYDRAGFEAVDP
                     ARSTHLLGLFERSHMEYEADRARDAGGEPSLAEMAAKAIDILARNPRGYFLMVEGGRI
                     DHGHHAGNAYRALTDTIALSDAVRAALAKVDLRETLVIVTADHSHVFTMAGYPARGNP
                     ILGKVVQPGASAPALAADGKPYTTLGYANGAGFHDGAQGIPPYPREPQTGRTQDLAAV
                     DTTHADFHQEALVPLESETHGGEDVAIFAGGPAAQHFHGVQEQSYVFHVMRAALGL"
     gene            11368..12219
                     /locus_tag="PLW13_07995"
     CDS             11368..12219
                     /locus_tag="PLW13_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQY70353.1"
                     /translation="MLRESPRILCLATLGAAPLAALVGAAAQAGFAGVTLRRHHIDQA
                     RAAGLGDDAIRELLQQLPLGAVMIEALTRWPADVARDSVAATQARALFECAVALGTRD
                     TCAVLLDDTPWPREELARAFRSLCRMAAGYGLRVALEFVPWSVIGNLEAAIDVIERAG
                     CANGGLLLDSWHWARANTPLDAPAALDPARVFMLQLSDAPATPAADLWHETLHERLLP
                     GAGTIDLARLLAALERRGVACPVAAEVFSDRLAALDPALAARELAASLEGLLSPKPAR
                     ETDAPSP"
     gene            12278..13555
                     /locus_tag="PLW13_08000"
     CDS             12278..13555
                     /locus_tag="PLW13_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY70354.1"
                     /translation="MRIDEADRATLAAWESELAARHAALAAAGLKLDVTRGKPNSAQL
                     DLSDRLDGVLDANYRSDDGTDLRNYGGLDGIPEAKQLFAPVLQVPVDNMLVGGNSSLT
                     LMWQCALYGMHFGFEGPDSAWRNGGPVKFICPVPGYDRHFGICEHLGIEMVTVPMTDN
                     GPDMDAVEALIDSDPAIRGMWVVPRFSNPTGAVCDEPTVRRCAALGKRAAPGFRLFWD
                     DAYAVHALEREAPKLAPVFEACRRAGTEDSVLLFGSTSKITHAGAGVAFLAASAANLA
                     AIRQHMGFSSIGPDKVNQKRHVAFLKDYAGVLDHMDGHAALLRPRFAAVLDALERELA
                     GTGMGSWSRPRGGYFVSFDTRPGLAKEVVRLAAEAGVKLTPAGATFPYGKDPADSNIR
                     IAPSFPTQAEIERAMEVFVVCVKLASVRQRLAG"
     gene            complement(13577..13936)
                     /locus_tag="PLW13_08005"
     CDS             complement(13577..13936)
                     /locus_tag="PLW13_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HQY70355.1"
                     /translation="MRMAAEPIYKVIFLNQNQVFELYARQIYQSDLYGFIEVEEYVFG
                     ERSQIVVDPAEEKLKNEFAGVKRSYIPMHAIIRIDEVEKEGSVKIRESKGSEGKVMAF
                     PLPGMRPQFGGEPPGDG"
     gene            14055..>14795
                     /locus_tag="PLW13_08010"
     CDS             14055..>14795
                     /locus_tag="PLW13_08010"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HQY70356.1"
                     /translation="MNDRPVRKVFVQTHGCQMNEYDSARMLDLLRESHGLQATDNADE
                     ADVLVLNTCSIREKAQEKVFHQLGRWRKLKEERPGLVIGVGGCVASQEGGDIGRRAPY
                     VDLVFGPQTLHRLPEMLDTALAGERRGPVVDISFPEIEKFDRLPEPSVNGPSAFVSVM
                     EGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAHLAAQGVREVNLLGQNVNAYRGRNHL
                     GEITDFAELLHLVAAVPGIERIRYTTSHP"
BASE COUNT         2340 a         5227 c         5009 g         2119 t
ORIGIN      
        1 cacgtccggg cctacgggga cgcggcgacg acggcgaggt cgatctcgcg cagcagcgcg
       61 atcacgtcgg ccgcatctgc ggcggcgaag tgcatttccg ccatttcggc gacggcgcac
      121 tgcgcgggca gcggcaggtc cgcgtcgagc agtgcacgca tgcgcggcag gaacacgaac
      181 tgcagccact gcgggaagtc cagcgtgtcg atgcagaacg gctgggtgct ggccagcgct
      241 tcgggcgagg gttcgccggc gtcccagagc aggcggctgc gcagcgccgc ctcgaggtcg
      301 atcaggagcg cggcgacacg gatgcggccg ttcatctcag gcgacctcgc ggcgaaacgg
      361 cggcagggaa tcgaggatcc tgcgcccgta ctggcgcgtg accacgcgac ggtcgagcaa
      421 cgtgatgcgc ccggtgtcct cctcggtgcg cagcagccgt ccgcttgcct gcaccagtcg
      481 cagcgccgcg tcgggcaccg agatctccat gaacgggttg ccgccgcgcg actcgaccca
      541 ctcggcgagc gccgcctcca ccggatcgtc gggcaccgcg aaaggcagct tcgcgatcac
      601 cacgtgcatg cagtagcccc cgggcagatc gatgccttcg gcaaagctgg cgagaccgaa
      661 tatcaccgag ccctcgccga catcgacacg ctcgcggtgc agccgcagca gttcctgctt
      721 ggcgaagtga tcctgcagca ggatgcggtc gcgcagcccc aggctcacgc ggtcgtagac
      781 ctcgcgcatc tgcttgcgcg aggcgaacag caccaggctc gcctcggcgc gatcgaggtt
      841 ggcgtcgagc cagtcagcga cctcgcgcgt gtgcgccacc ggatcgcccg ggtcggcggc
      901 aagccgcggc accgcgaggc aggcgcggcc gcggtagtcg aagggactgg gcaccgtcgc
      961 gaacacgcaa tctgcgggca gccccgcgcg cagcacgatg cggtcgaact tgccgagcgc
     1021 cgtcagcgtg gccgaggtca gcacggcgcc cgccgcacgc gtccagatgc tgtcgcgcag
     1081 cacgttcgcg gccagcaccg gcgcggcgca gacctcgaga tccgcgctcg actgctgctc
     1141 gctggcgcgg atccagcgcg cctgcggcgc cgcgccggcg ggatcgggct cggcccagga
     1201 tttccacagc cccgccagcc gctgcacgcg cccgatcgag agccccattg ccgcgtagcg
     1261 atcctgcgcc gccgcgccgc ccgtatcgag ctggcgcgac agttcgtcct cggcgccttc
     1321 cagcgcgtcg ctgagcttgc gcagcgcatg gtgcagcgtg gcggcgattg ccgcgacatc
     1381 ggccggcacc acgccatgcg gcaggcggtg ttcgacggcc gtgtcgccct tgcggcccgc
     1441 tcccgccagc agggcgccca gcagccgtcc ggcggcgctc aacccgtggt cgagttcctc
     1501 gcagagcgcc ggcacttccg cgaggcgccg acgcagggcg tcgctcgagc gcggctcggc
     1561 catcagctcc gtcgcgagcc ggcggcaatc ctcggcgtgc tcgcgtgcga gccccagccg
     1621 cgcgcggaac gacaggtggt tgcaggcctt gtccggcagg tggtgcgcct catcgaacac
     1681 gtagatcgca tcttccggcg cgggcagcac ggcgccgccg ccgagcgcga ggtccgccag
     1741 caccagatcg tggttggcca cgatgcagtc gacgccgtcg agcccctcgc gcgcggtgaa
     1801 gaacgggcac tggctcacgt aggagcaacg ccgcccgctg cactggtggt ggtccgtggt
     1861 catcagggcc cagcgcgagt ccggtagcgc cgcgggccag gaatcgcgat cgccggccca
     1921 gctgccgtcg gtcaccgccg tgtagagctc gcgcaccagc tcgcgggtct cctggttcac
     1981 gcttggcacg atctgctcga ccagcgcgag ctgcgcgccg tcttcatcgc gcgcgccgcg
     2041 cagcagagag tcgaggcgcg cggggcacac gtagcgcccg cgccccttgg cgagcgcgta
     2101 gctgaagtcg agcgcgccgg cggtcttcag gtcgggcagg tcacggtgga agagctgctc
     2161 ctgcaacgcc accgtcgccg tggagatcac cagcttcttg ccgagcgcct gcgcgatggg
     2221 gatcgcggcg accgcgtatg ctatggtctt gcccgtgccg gtgccggcct cgaccacaca
     2281 gatcgggctg gcctcgttgt cgggatcgtt cgagcgcgcc agggcgttcg ccacctgcgc
     2341 gatcatcgcg cgctggccgc tgcgcggcct gaggccgcgt gcgttcagcc agttgcgata
     2401 cgcgccctgg atggcattct tgacgtcgtc gttcagcaag cgaggtcccc gcgcaggaag
     2461 gaagcggact atagcagggc aggcgtcgcg cggcgctcgc cagccgccac ggcggcaatt
     2521 ctccggaagc aagcaagcga tgatcgaaac actgtcccac ggcatcacct gcatcgacac
     2581 gggttaccac cgcgccggcc tcgcggcctg ctacctggtg gtcgatgacg gcgaggcggc
     2641 gctgatcgaa accggcaccg cgctcaacct gcccgccatc ctgcgggccc tgacggattg
     2701 cggcgtcgcg ccggagcagc tgcgctacat catgccgacg cacgtgcacc tcgatcatgc
     2761 cggcggcgcc ggcgcgctgc tcgcgcgctg cccgcaggcg acgctggtgg cgcatccgcg
     2821 cggcgcccgc cacctggtcg accccgagcg cctcatcgcc ggggccaagg cggtgtacgg
     2881 cgaggaatcc ttcacccgca tgtacggcga tatcgtgccg gcgccgctcg agcgcgtgcg
     2941 cgaggctgcc gacggcagcc gctggacgct gggacggcgc gaactgctgg tacgcgacac
     3001 gcccggacac gcgcggcacc atttctgcgt gtgggacgaa acgagccgcg gctggttcac
     3061 cggggacact ttcggcatcg cctaccgcga gttctactgc ggcggcgagc cctacctgat
     3121 ccccaccacc acgccggtgc agttcgagcc cgaggcgctg ctcgcttcca tcgagctgct
     3181 gatggcggcc gagccgcact actgctatct cacgcatttc gggcgcatcg cggcgagcgc
     3241 gcatcacgcc ggatcgctgc gccgccaggt cctcgactac gtgcgcctgg ccgagggcat
     3301 cgatatcacc gatccccacg cgcaagccgc gctcaccgag gcgctgacaa cctacaccct
     3361 cgagggcctg cacgcggccg gcagcacgct gcccgtcacc acgctgcgcg cactgctcgc
     3421 gctcgacatg gaactgagcg cgcagggact ggtgcactgg atgcgccact cctaggtcgg
     3481 cattcatgcc gacatgcgcc cgcatgcacc actgtcgcca tgaatggcga cctacggcgc
     3541 gtcgagggcc gcgcggttca gcaggggatt ggcatacatc gcgagcaggt agggacggtg
     3601 cggcgcggga agcgcttcga ggcgcgcgcg cagctcggca tccgccgcag cgaccgcggc
     3661 atcgtcggcg aatccctccg gcagccggcc ctgcagcatg cggaacgccg cgatattgct
     3721 ggagtggacg cggtacagcg ccagtacctg gcggtcgatc tccgcggcta cctcgtcggg
     3781 gcccgcgaag tcggcgtcga gcggttcgcc gaagctcaca tgcaccgcgc cttttggtga
     3841 agtgatgctg tgcgcgatgc tggccacgtc ctcgtgctcc tccttctcgt aatcgccttc
     3901 gctcgcgcgc aggtacagct cgcgggcctt ggcgccgtcg cagggatccc attcgtagga
     3961 tatcgaaacc ggcacgatgt tgagcgcgcg caggtgcgct gcaatcggcg tctcgcgcgc
     4021 cttgctgatg cacagcatct tgatgatcgc cggctcggtg cggtcccagc catccttggc
     4081 gcggccctcg cgctgcgcga tccagatcga gctgcactcc tcgagcagcg aatgccggat
     4141 atagcccgac agttcctgca gggccgcgag catctgtcgc ggcccctttg ccgagcggcg
     4201 cacgatgaag ctcttgttga gtcgcatcag gtccgagaca tagtccttgg tcagcaggtt
     4261 gtcgccgatg gcgatgcgca cggtgtcgca gccgttggca tacagcgcgt agttcacgaa
     4321 ggccggatcc agcgcgatgt cgcggtggtt gccgatgaaa aggcaggccc gggcgggatc
     4381 caggcgctcg agtccgctca ccgtgaacgc cgtggtgctg gactcgatca tcgcggtcat
     4441 gtacttctcg acgaccttct ggaagccggc gacgctggcc acgccagcga gctggcgccc
     4501 gaggtagagg cgcacgatcg ggcgcagcag cgcacccagc gcgccgccgg cacgcggaaa
     4561 gcgaaggcgc gtgagcgccg ctatgaactc ggcattgcgc agcagccggt ccagcacgcc
     4621 gcgcacctcg tcgtcgttgt agggtctgat ctgcgcgaac cggtcctggc tcattgcgtc
     4681 ccctgggtct gtggctgatc gcccgccgcc gggcttttcg gcgcgcgatt atacgctacc
     4741 attcccgcct cccccgccat gaatgccgcc atgtccgacg acccgatcct gttctcccgc
     4801 gacggccaca tcgcccgcat cacgctgaac aacccggcgc agcacaatgc gctgggcgcc
     4861 gccgagatcg gccgtttccg cgattgcctc gacgccgtcg agaccgatcc ctcgctgcgg
     4921 gtgctgctgc tcaccggcaa cggcgagcgc accttttgcg cgggcgcctc gctgaagcag
     4981 ttccggagcg gggacatgag cggcgaactg ttcagcacgc tgaccgaccg gctggcggcg
     5041 ctgccgatcc cctccgtgtg cgcgctgaac gggaacgtgt tcggcggcgg agcggaaatc
     5101 gcgctgtgct gcgattaccg catcggcgtg gacgacctgc ggctgctggt gccggcggcg
     5161 cgcatcggca tctgctaccc gctgaacggc ctgcagcgct acgtcgacaa gctggggctc
     5221 ggcgcggcga aacgcatcct ggtggccagc gaggaactgg cggcggaaac gctgctcgcc
     5281 atcggcttcc tgcaccgcgt cgtggcgcgc gagcgactcg acgccgagag cgacgccttc
     5341 gcacgccacc tcgccgggct cgcgccgctg gcggtgcgcg cgatgaagca gattctcggc
     5401 ggcatcgcgc gcggcgacct ggacagcgca ggcgcggccc gcctggtgca gcagtgcacg
     5461 cagtcgcgcg acctgcagga aggcctcgcg gcgcagcgcg agaaacgcgc gccgcgtttc
     5521 accggggaat gagcatcgcg ccgccgcgca tgccgcgccg gtacgcggcc ggcgagcggc
     5581 cggtccagct gaggaaggcg cgacggaaat tcgagacatc gttgtagccc agcatgtcgg
     5641 cgatctccgc gatcgagagc atgctgtcgc gcaggtaggt ctcggcgagc tggcgcctca
     5701 actcgtgcac gatgcgctgg tagttcgtgc cctcctcgtc gagccgccgg cgcagcgtgc
     5761 gcgggctcac gtgcaggtgg tcggcggcct cctcgaacgg gggaaagcgc cccggcgtcg
     5821 ccagcaggta attgcggatc tcggtggtga agccgtcgct gcggttgagc gcggccagca
     5881 gctcgtcgca ttgctggcgg tagagcttca gcagcgtggg attggcgaac tgcagcggca
     5941 cgtccagata gcgccggtca acggcgagcc ggctgtcggg cgcatcgaag cgcacgtccc
     6001 ggcagatcag ccggcgatac tcctcgccat accccggatc gggataggcg agcgtggcgc
     6061 tgagcgcgtc atcggacagc gcgcacagcc ggcgcagcga ggctgccgcc gccgcgaaga
     6121 aaatctcgca gtcggccggc tccgccgcct cgtgcagcat gcctccgact aggcggatcg
     6181 agaccgcgtc gcgcccggat tcgacctcga tgcggaagct gccgctgatc aggcggtggt
     6241 aatgccccat cacctccatc agttcctgca cgttgcgaca gcacatcagc gcgtagccga
     6301 gctcgccgag cgcggccacc gagatgcggc tgccgaaatg cagtgcgaaa cccggatcgc
     6361 cggaacggcg caggcagttc tccagcatgc gcgtctggtc gcgcagcgag atgcgggtat
     6421 cgggcgcacc gagcgctccc tcgtcgatcg aggtgccctc cagcacctgg gacggggtga
     6481 tgccgaaatc ggcggcgacc tcaaccatga tcgccccgat cggcgcgggc aggtcgtggc
     6541 gcggaaccct caggcgatgc accatcattg gccttgattg accattttgt ggcacaagat
     6601 aaccttgttc gacaaacgcc ggcaaccaag aatgaacttc ctccggcgcc ggccggacaa
     6661 aagacagaca acagcaatca cggaggacag cccatgggcg ttcaattcgt catcgacacc
     6721 ccgagcgaag tccgttactt cgaccaggaa gcgcagcgca tggacgcggg caccgcgcag
     6781 caggtcgccg agatcttcca gactccgctg accggcagct acaactggga ctaccgcgtc
     6841 caggacgacc gcatcaagaa gctctacgaa ctgggcaagc agctcaactg gaacgtcgag
     6901 accgacatcg actggggcaa gcggcgcacg cgtctcacgc ccgagcagcg cgacgcgatc
     6961 gtcgccttca agggctacgg tccgtacgaa gcgctcagcg aggagcagaa gcaggagttc
     7021 gcggagcacg agaatgtatg gacgctgtcg cagttcctgc acggcgaaca gggcgcgctg
     7081 ctggtggcct cgcagctcac gagctgcgcg ccgacgctga acgccaaact ctacgcggcc
     7141 tcgcagacct tcgacgaggc ccgccacgtc gaggtcttca acaagtacct gcagaccaag
     7201 gcaggttata tgtacccggt ggatccggcg ctgaagagcc tgctcgacaa gatcctcacc
     7261 gatccgcgct gggacctgaa gttcatcggc atgcagctga tcatcgaggg gctggcgctg
     7321 tcggccttca ccacgatgaa ggcaaccacg cccaagggcg aactgctgaa cgacgtggtc
     7381 gagctggtgc tgcgcgacga agcgcgccac gtgactttcg gcatcaacta cctcgaggag
     7441 catctcagga acctgagcga ggccgagcgc gaggagcgcg cgcgcttcgc ctacgaggcc
     7501 tgcgtcgtgg cgcgcgagcg catgatcccg aaggacgtgt tccaccactg gggctggaac
     7561 gtcgaggagg cgcggcgcat ctacctcgaa ggcgagttcc gccagatgtt ccgcaacctg
     7621 atgttcatgc gcgtgatgcc gaacctgcgc cgcatcgggc ttttgaccga ctcggtgcgc
     7681 ccgctgttcg agcagctcgg catgctcgac tacgagaacc tgaaggacga cggcgatatc
     7741 gactgggccg agctgtcgcg cccgctgttc gacacggccg cctgagcctt cactgcggcg
     7801 caccgccgat gcggaatgca tccgcatcga ggtgcaccgg gaatttctcg cgacaatccg
     7861 cgatcgttgc cgccgcgagc gtcacgcggt gcacgcccgc ggcacagccg gcatccagca
     7921 ccaccctgcc ccacgggtcg atcaccaggc tgtcaccggc gtagtcgact cccttgccgt
     7981 cgacgccggt gcggttcacg ccgatgaccc aggcctggtt ctcgatcgcg cgcgcgacga
     8041 gcagcgcgcg ccagtgctcg cgccggcgcg ccggccagtt cgccacgtac agcagcgcgt
     8101 cgtagtcgcc gcggttgcgg ctccacaccg ggaagcgcag gtcgtagcag acctgcgggc
     8161 acaaccgcca gccctgccat tcgatcacgc gccgctcgct gcccgccaca tagccgtgat
     8221 gctcgccggc catgcggaac aggtggcgct tgtcgtaatg cacgagccca ccgtccgggc
     8281 gcgcgaacag gaagcggttg cgaaacgccc cgccctcctc gatcgcgagg ctgccggcga
     8341 tggcgcatcc gtgcagcgcc gcctgctcgc gcagccagcc caacgtgggg ccatccatgc
     8401 cctcggcgca ggccgccggc tccatcgtga agccggtggc gaacatctcc ggcagcagcg
     8461 ccagcgcgct gccggcggct gccggcagca ggcgggcgaa ttgcgcgcga ttggccggcg
     8521 cgtcctgcca cgcgagatcc tgctggaaga tcgtgacgct cagatcctgc atagcaactc
     8581 cgccgcgcgc accagcgtct cgtcctgctt ggcgaaacag aaacgcagca gccgcaggcg
     8641 cggcgggcgc tcgtagaaca ccgacaccgg gatcgcggcc acaccgtggc ggctgacgag
     8701 ctcctcggcc atcgccacgt cgtccgcgtc gccgatgccg gaatagtcga gcagctggaa
     8761 ataggtgccg gcgcagggca gcgcacgcca gcgcgagccg gcgagcaggc ggcgcaacag
     8821 gtcgcgcttg gcctggtaga acgcgggcaa tcggtccacg tgagccggtg ccccggccat
     8881 gaaatccgcc agcgcgtgct ggatcggcgt gaccgccacg aaggtcacga actggtgcac
     8941 gcggcggaac tcgcgcgtca gctcgggcgg cgccacgcaa tagccggtct tccagccggt
     9001 ggcgtggtag gtcttgccga aggagaacac cgcgaaggca cgttcggcca gcgcgggccg
     9061 cgccagcacg ctgtggtgcg ccgcgccgtc gtagaccagg tgttcgtaga cctcgtcgga
     9121 cagcaccagc aggccatgct cctgcaccag ttcctcgaga cgatcgaggt cggccgcgcc
     9181 gagcacgctg ccggtgggat tgtgcggcga attgatcacg atcatccgcg tgcgcggcgt
     9241 gagcgcctcg gccacgcgct gccagtcgat cgcgaaggcc ggcggcagca gggggatgcg
     9301 caccgcgcga ccgcccgcga gacgcaccga gggttcgtag ctgtcgtagc tggggtcgaa
     9361 caggatgact tcgtctcccg cgcgcaccgt cgccatgatc gcgcagaaga tggcttccgt
     9421 ggcgcccggg accacggtca cctccgcctc cgggtcgacg cggcagccgt agcagcgccc
     9481 cagcttcagc gcgatctgct cgcgcaacgc cagcacgccc gccatcggcg cgtactggtt
     9541 gcgcccctcc gcgacgtggc gcgccagcgc ctcgccgagg cccgcgggca gcgcgaaatc
     9601 gggaaatccc tgcgacaggt tgatcgcgcc gtgcgcctcg gccttgcgcg acatcaccgt
     9661 gaagatcgtc gtgcccacgt cgggcagctt ggactggggc aggggcatca cgtcaccgcg
     9721 gccgcgcgcg gcagctcgtg caggtgcaaa ttcatcgcct ggtactcctc gatgcggatg
     9781 ccgcgggaag gcaccatgac gctaccacgc cgccgcgcgg cgggaaacct gcaacgcctg
     9841 tacgcgccga ttgacgcggc cgaggcagcc cgcgacagtg ccgtcaccat gccggcacga
     9901 tcgccggcca ccacaccgcc gccgcgcgcg atccggggac aagccatgat ccatgcccgc
     9961 cactgcgccg ccctgctgct cggcctcgcc ggctccggca tggcctgcgc caacgctgag
    10021 ccgccgagcg cgccagtcga ggactgggcc gccaggggag ccgttgcact cgccgagtcg
    10081 cgcgccctct tccccggcat gccggagcgc gcgcgcaacg tgatcctgtt cgtcggcgac
    10141 ggcatgggca tcaccacgat caccgcggcg cgcatcctcg agggacagca gaagggcggc
    10201 agcggcgagg agaactcgct cgccttcgag cggctgcctt ttgtcgcgct gtcgaagacc
    10261 tactccgcca accagcaaac gccggattcc gcgccgacca tgagcgcgat gatgaccggc
    10321 aacaagacca gcgacgggct gatcagcatc gccgccacgg tggcgcacgg cgagcccgcg
    10381 cgcgaggcgg tcgcggctgc cgcgcaaccc acgctgctgg aactggccga gggcgcnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg cacgcgcgac cccgaggacc
    10561 acggcgcgcg cggcgtgcgc cgtgacgggc gcgacctcac gcgcgaatgg aagcgcaagt
    10621 tcggcggaca gtacgtgtac gaccgtgccg gcttcgaggc ggtcgatccc gcgcgcagca
    10681 cgcacctgct ggggctgttc gagcgctcgc acatggaata cgaggccgac cgcgcgcgcg
    10741 acgccggcgg cgagccctcg ctggcggaga tggccgccaa ggcgatcgac atcctcgcgc
    10801 gcaacccgcg cggctacttc ctgatggtcg agggcggacg catcgaccac ggccaccacg
    10861 ccggcaacgc ctaccgcgcg ctcaccgaca ccatcgcgct gtcggatgcc gtgcgcgcgg
    10921 cgctggccaa ggtggatctg cgcgagacgc tggtgatcgt caccgccgac cacagccacg
    10981 tgttcacgat ggccggctac ccggcgcgcg gcaacccgat cctcggcaag gtcgtgcagc
    11041 ccggcgcgag cgcgcccgcg ctcgccgccg acggcaagcc ctacaccacg ctcggctatg
    11101 ccaacggcgc cggcttccat gacggcgcgc aggggatccc gccctacccg cgcgagccgc
    11161 agacgggccg cacgcaggac ctggcggcgg tcgataccac gcacgcggat tttcaccagg
    11221 aggcgctggt gccactggag tccgagaccc acggcggcga ggacgtcgcg atcttcgccg
    11281 gcggccccgc ggcgcaacac ttccacggcg tgcaggagca gagctacgtg ttccacgtga
    11341 tgcgcgccgc gctggggctg tgacgcgatg ctgcgcgagt cgccccgcat tctctgcctc
    11401 gccacgctgg gcgccgcgcc actcgcggcg ctcgtgggcg ccgcggcgca ggcgggcttt
    11461 gccggcgtca cgctgcgccg ccatcacatc gaccaggcac gcgcagcggg tctcggcgac
    11521 gacgcgatcc gtgaactgct gcaacaactc cccctcggcg cggtgatgat cgaggcgctg
    11581 acgcgctggc cagcagacgt ggcgcgcgac agcgtggcgg cgacgcaggc gcgcgcgctg
    11641 ttcgaatgcg ccgtagcgct gggtacccgg gacacctgcg cggtgctgct ggacgacacg
    11701 ccgtggccgc gcgaggagct ggcgcgcgcg tttcgctctc tgtgccggat ggcagcgggc
    11761 tacggcctgc gcgtggcgct ggaattcgtg ccgtggtcgg tgatcggcaa cctcgaggcg
    11821 gccatcgacg tcatcgagcg cgccggctgc gccaacggcg gcctgctgct cgacagctgg
    11881 cactgggcgc gcgccaacac ccccctggac gcgcccgccg ccctcgaccc cgcgcgcgtc
    11941 ttcatgttgc agctgagtga tgcgcccgcc acaccggccg cggacctctg gcacgagacc
    12001 ctgcacgagc ggctgctgcc gggcgccggg accatcgatc tcgcgcgcct tctcgccgcg
    12061 ctcgaacgac gcggcgtcgc ctgcccggtc gcggccgagg tcttcagcga ccgcctcgcc
    12121 gccctggatc ccgcgctggc tgcacgcgaa ctcgccgcga gcctggaggg cctactttca
    12181 ccaaaacccg cccgtgagac ggacgccccc agcccttaga atgcgctcgc ccgcagcagg
    12241 gcacatcatc acaccgtgac actggaggat accggcagtg cgtatcgacg aagccgaccg
    12301 cgccaccctg gccgcctggg aaagcgaact cgcggcgcgc catgccgcgc tggccgcggc
    12361 gggattgaag ctcgacgtga cgcgcggcaa gcccaacagc gcgcagctgg acctctcgga
    12421 ccggctcgat ggcgtgctgg acgcgaacta ccgtagcgac gacggcaccg acctgcgcaa
    12481 ctacggcggc ctcgacggca tcccggaagc gaagcagctg ttcgcgccgg tgctgcaggt
    12541 gcccgtggac aacatgctgg tcgggggcaa cagcagcctc acgctgatgt ggcaatgcgc
    12601 cctctatggc atgcatttcg gcttcgaggg tccggactcg gcctggcgca acggcgggcc
    12661 cgtgaagttc atctgcccgg tgcccggcta cgaccgccac ttcggcatct gcgaacacct
    12721 gggcatcgag atggtcacgg tgccgatgac ggacaacggc ccggacatgg acgcggtcga
    12781 ggccctgatc gacagcgacc ccgcgatccg cggcatgtgg gtggtgccgc gtttctcgaa
    12841 cccgaccggc gcggtctgcg acgagcccac ggtgcgacgc tgcgcggcgc tcggcaagcg
    12901 tgcagcgccc ggcttccgcc tcttctggga cgacgcctac gcggtgcacg cgctggagcg
    12961 cgaggcgccg aagctcgccc cggtgttcga agcctgccgg cgcgccggca ccgaggacag
    13021 cgtgctgctg ttcggctcga cctccaagat cacgcacgcc ggcgcgggcg tcgcgttcct
    13081 ggcggcatcg gcggcgaacc tcgcggcgat ccgccagcac atgggcttca gcagcatcgg
    13141 cccggacaag gtgaaccaga agcgccacgt cgccttcctg aaggattacg ccggcgtgct
    13201 cgatcacatg gacggtcatg cggcgctgct gcgcccgcgt ttcgccgcgg tgctcgacgc
    13261 gctggaacgc gaactcgcgg gcaccggcat gggctcgtgg agccggccac gcggcggcta
    13321 tttcgtgtcc ttcgacacac gccccggact ggcgaaggaa gtggtgcgcc tcgcggccga
    13381 ggccggcgtc aagctgaccc cggccggcgc cacattcccc tacggcaagg atccggcgga
    13441 cagcaacatc cgcatcgccc cgagcttccc gacgcaggcc gaaatcgagc gcgcgatgga
    13501 ggtgttcgtg gtgtgcgtga aactggcgag cgtgcggcag cggctggcgg gctgatccgc
    13561 ccggacccgc gggagatcag ccgtcgccgg gcggctcgcc accgaactgc gggcgcatgc
    13621 cgggcagcgg gaaggccatg accttgcctt cgctgccctt ggattcgcgg atcttcaccg
    13681 agccttcctt ctcgacctcg tcgatgcgga tgatcgcgtg catcgggatg tagctgcgct
    13741 tcacaccggc gaactcgttc ttcagctttt cctcggccgg atcgaccacg atctggctgc
    13801 gttcgccgaa tacgtattcc tcgacctcga tgaaaccgta cagatcgctc tggtagatct
    13861 gcctggcata taactcgaac acctggttct ggttcagaaa tatgactttg tagatcggtt
    13921 cggccgccat gcgcatatcg ctcgtcggtg ggtaaatgca agggggcgcg aaggatacca
    13981 cagcccccgc ctgtgcatcg cccgccgcgg ccgctataat ttccgccctt ttttcgcacc
    14041 tcctccccac gccaatgaac gaccgtcccg tccggaaagt cttcgtgcag acccatggct
    14101 gccagatgaa cgagtacgac tccgcgcgca tgctcgacct gctgcgcgag agccacggcc
    14161 tgcaggcgac cgacaacgcg gacgaagcgg acgtactggt gttgaacacc tgctccatcc
    14221 gcgagaaggc gcaggagaag gtgttccacc agctcggccg ctggcgcaag ctgaaggagg
    14281 aacgcccggg cctggtgatc ggcgtgggcg gctgcgtggc cagccaggag ggcggcgaca
    14341 tcggccgccg cgccccgtat gtggacctgg tgttcggccc gcagaccctg caccgcctgc
    14401 ccgagatgct cgacaccgcg ctcgccggcg aacgccgcgg cccggtggtc gacatcagct
    14461 tcccggagat cgagaagttc gaccgcctgc cggagccctc ggtgaacggc ccgagcgcct
    14521 tcgtctcggt gatggaaggc tgcagcaagt actgcagctt ctgcgtcgtg ccgtacacgc
    14581 gcggcgagga agtcagccgc ccgctcgacg acgtgctcgc cgagatcgcg cacctcgccg
    14641 cccagggcgt gcgcgaggtg aacctgctcg ggcagaacgt caacgcctac cgcggccgca
    14701 accacctggg cgagatcacc gacttcgccg agctgctgca cctggtggct gcggtgccgg
    14761 gcatcgagcg catccgctac accacctcgc acccg
//
LOCUS       DAORUQ010000071        14740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3887_length_14740_cov_6.131290, whole genome shotgun sequence.
ACCESSION   DAORUQ010000071 DAORUQ010000000
VERSION     DAORUQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_14740_cov_6.131290"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLW13_08015"
     CDS             complement(<1..498)
                     /locus_tag="PLW13_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY70357.1"
                     /translation="MSAFHRVVPPNRRGRDFVVGDLHGYLGALQDALARAGFDPGCDR
                     LFSTGDLIDRGPDSPGCAALLGEPWFYAVRGNHEDMLLRVLAGEILLLDVWRQNGGDW
                     ALDGWEPNAAGRRAGALCARMPWAITVEHRSGRRFGICHGQCPVAEWGEIASIARDEK
                     LQQEML"
     gene            complement(495..1859)
                     /locus_tag="PLW13_08020"
     CDS             complement(495..1859)
                     /locus_tag="PLW13_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQY70358.1"
                     /translation="MLQLALPLVAGQFAIIGMGVTDVVVAGHAGTNDLAGVTIGFYAW
                     DLSMLLVFGTMLANSALIGHAYGAGNVAAIRRQFQQAMWLALPLGVLSALAIFGAMHA
                     VRLLDAEPQVTRVAAGYLLPTIGTALLLPVAICFRTTNEGLGLTRPVMWLSLGAFVIN
                     IPLDYALVLGAFGLPRLGGAGCGWATMISFALLVFAWTLYALFAPALRGYRLWQGFGA
                     PIVRELRAMLALGLPIGLSLLAVGGFFAVLPMLMASLGAVAIAGHAVAITVDTLMLTI
                     PLGVGQAMSVRVAHELGGGNPRGARDVCATGMLLLVGIALLQAGAIIALRHPITSLFT
                     RDAAVAELGTMLLLFAAAYRVFDSVQIGAGMALRGYKDTRVASAINIAAYWLFGLPLC
                     WTLAMGSPWSAGLGVAGFWIGMLAAIGVAALAIAWRLARTSRRHLRAAAPAPGASAAQ
                     AVGA"
     gene            complement(1936..2697)
                     /locus_tag="PLW13_08025"
     CDS             complement(1936..2697)
                     /locus_tag="PLW13_08025"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQY70359.1"
                     /translation="MFEFWRRRKLARHAFADRDWEAVCARLPLLARLDAAGRQRLRET
                     ATLFLADKIVAPAAGFSLQPRQRIEIAIAAALPVLHLGLGAYRSFRGVIVYPDEFLAP
                     REEIDEAGVVHTGHERVAGESWDAGPMLLSWADVEASREADGYHVVIHECAHKLDLGD
                     GDMNGVPDLSGSGVSGAEWRRVMTAAWERTGADWDAYGETEVDDYALESPAEFFAVLS
                     EHFFTIPEHLQEVLPEAYALLARFYRQDPLHGQHA"
     gene            complement(2720..3142)
                     /locus_tag="PLW13_08030"
     CDS             complement(2720..3142)
                     /locus_tag="PLW13_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY70360.1"
                     /translation="METVLVPVDGSDASARAAAWAATYAAGRGAQLVLLHVHAARGSE
                     TLALGALDAAQIEEAERRIAGPSFDKARAAIGPAAVARTLVSIGDPAEEIVALARRER
                     ASLIVMGSRGLSPVRELLLGSVSEKVIRHADCAVTVVR"
     gene            complement(3171..5210)
                     /locus_tag="PLW13_08035"
     CDS             complement(3171..5210)
                     /locus_tag="PLW13_08035"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQY70361.1"
                     /translation="MPGMQQLPPLLGALGLACALIIYLLLTRRPEGEGKVRKIAAAIH
                     LGAMVFLQREYKTLALFAAVLIALLWYFLGANTALAFLCGAACSALAGWLGMYAATRA
                     NVRTTTAAHTQGAAAALSVAFYGGSVMGLCVASLGLLGLGSLYLVFGGDPHTAHAIHG
                     FGMGASTVALFSRVGGGIYTKSADVGSDLVGKLEAGIPEDDPRNPGVIADNVGDNVGD
                     VAGMGSDIFESYCGAMIATIAIASTMAFGQLGMLAPQLDEAPARAALMFLPLALASTG
                     LLASVTGIVVVRFLCAQRPALALRSGTIAAPLLFIVAAWFLIRALGIDAAVWWSVICG
                     AAGGIVIGLVTEWYTSSTPVLRIAAAGQTGPATVLITGLAVGMQSVTVPMLAICAIIY
                     VSTGFADLYGVGIAAVGMLATVGITMAIDAFGPVADNAGGIAEMAGLGPETREITDSL
                     DALGNTTAAIGKGFAIGAAALAALAIIAAYVETVDAHWAATGQGTIDLSLNNPHVLVG
                     LFIGGIVPFLIASMAMTAVGDAAMEMIVEIRRQFREIPGLMEGTAEPDTARCIDIATA
                     AALRRMLLPGLIAVATPPLVGFGIGAEALGGALGGGLLACVLLALMMSNAGGAWDNAK
                     KHVEKGHYGGKHSPVHAATVVGDTVGDPFKDTAGPSMNILINVMAIVSLVIAPLL"
     gene            5432..5629
                     /locus_tag="PLW13_08040"
     CDS             5432..5629
                     /locus_tag="PLW13_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQY70362.1"
                     /translation="MPPKIRELILQLQRAGFADRGGKGSHRNFVHPDASRPVTLSGNP
                     GDDARQYQVRAVRAAIEEIER"
     gene            5626..5865
                     /locus_tag="PLW13_08045"
     CDS             5626..5865
                     /locus_tag="PLW13_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70363.1"
                     /translation="MKDAARYVKVVEWSEEDQCFVGSAPGLVFGGCHGDDEMAVFAEL
                     CLIVDEAILLYRREGKPLPPPTSGRDFVNKMQCVA"
     gene            complement(5982..6893)
                     /locus_tag="PLW13_08050"
     CDS             complement(5982..6893)
                     /locus_tag="PLW13_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HQY70364.1"
                     /translation="MAIPDYQSCMLPLLEFASDQQDHKVSEAVEALSDLFGLTPSERT
                     ALLPSGAQKIFHNRIGWARSYMKKAGLLDSPKRGLFRITERGLNVIAQKPSRIDVKFL
                     GQFPEFVAFRESGKNDGSYSSPNNASPAEITPEETLELAYQDLRKEIAQEVLSRIISC
                     SPTFFENLVVELLVKMGYGGSRADAGERIGQSGDGGIDGIIKEDKLGLDVIYIQAKRW
                     QGNVGRPEIQKFVGALQGQRARKGVFITASSFTSDAIDYVGKIDTKVVLIDGNMLSSL
                     MIDFDVGVSASATYVIKKIDSDYFEEL"
     gene            complement(6899..7708)
                     /gene="hisN"
                     /locus_tag="PLW13_08055"
     CDS             complement(6899..7708)
                     /gene="hisN"
                     /locus_tag="PLW13_08055"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HQY70365.1"
                     /translation="MNHTDAGAALAFAEELVQEAGRIALRHFRQPLDVANKLADGRFD
                     PVTIADREVESFLLERIRARYPDHGIVGEEHGAVAGDSRYSWIIDPIDGTRAFISGVP
                     AWGILLGLRDAGRCIGGLMHQPYLEETFTGSPAGGWLSRRGERRRLQTRASARLVDAI
                     LYCTHPTIFPSAGELAAFEHVAAHTRLMRYGGDCYSYCLLALGHIDLVIEGGLQAYDI
                     QPLIPIIEAAGGVVSGRHGEDASGGGFVVAAANRALHAEALGILRSAENKD"
     gene            complement(7705..8346)
                     /locus_tag="PLW13_08060"
     CDS             complement(7705..8346)
                     /locus_tag="PLW13_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQY70366.1"
                     /translation="MIAGISAAYALLAAVYLLLFGYNDSWAGAIVKMLPILLLAAASA
                     RLMRGTWRRWMVVALLFSALGDWLLGMNGVVGNLFAAGLGSFLVAQLAYAQLFWRQRA
                     PDRRRRWLAACYLPVAAGLAWLVLPVAGEMAVPVALYMLAITAMVTGAALANRPLLLL
                     AGALAFAFSDATIAINKFVSPVPQAGLVIMLSYYLAQWLLWRGALREPKEISA"
     gene            complement(8343..9899)
                     /locus_tag="PLW13_08065"
     CDS             complement(8343..9899)
                     /locus_tag="PLW13_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQY70367.1"
                     /translation="MTDKHNTLTRRDFVQGAATLAAGAALFPPGASAAGTSKMAIRDY
                     VAHDAMGLAALVKRGEVSAAELLELAIARTEAVNGSITAVCLKHYDEARAALKSIDPN
                     APLAGVPFLLKDLGVYLKGTITSNGSRFFADRVATQTSTVVQRYQAAGLNIFGKSASP
                     EFGQIPNTVSTLWGKTRNPWNLEYSAGGSSGGAAAAVAAGILPAAHATDGGGSIRFPA
                     AMCGLVGLKPTRARTPLGPDKSEGWSGLSSGHVVSRTVRDTALLLDLTQGPEPGAAYW
                     PPAPADSYMNELAREPGKLRIALITRAPTGAAVHQDNLDAIAKAADLCRSLGHEVREA
                     ELPEPFSKLMESFGFLSMVGVSAQIRERARELGREPGPDDLEPVVLGYYRQAPSISAL
                     DYEAARQSVQRLGFTMNTFMQDIDVLLMPMAPRAPWKVDDVTLVMPTEEFYKKAVGYS
                     DFSAIFNATGQPAMTLPLHENAAGIPVGTHFAARYGDEKTLIRLAAQIERAAPWDKRV
                     SPMLAQAMKA"
     gene            complement(9889..11007)
                     /locus_tag="PLW13_08070"
     CDS             complement(9889..11007)
                     /locus_tag="PLW13_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70368.1"
                     /translation="MDFEYSEEQQLLRDSVERFVREHYTFDARRAIVAGARGFSAQHW
                     QLFAELGWLALPVAEDDGGLGGNLTDLAILFEQFGRGLVVEPVLATVVLGAGLVSELG
                     SLVQRESMLPAIMAGEMQLALAFAEPGSRYSLARIATTAGRNDEGWILDGHKCVVLNA
                     PNADTLLVSARDAGGAIGIFRVAPASAGLRMHGYATIDGGRAADIELDAVRVPADALL
                     GTAAIGLAALASVIERATLCVCAEAVGAMDALLGKTVEYTKTRRQFGVAIASFQALQH
                     RMAEMFIELEQSRSILMMALMAAEAGEALTRPVSAAKSRIGRAMRRVGQEAIQLHGGI
                     GVTDELDVGHYFKRLTAIETLFGNTAFHLQRFGASHDR"
     gene            complement(11184..12383)
                     /locus_tag="PLW13_08075"
     CDS             complement(11184..12383)
                     /locus_tag="PLW13_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70369.1"
                     /translation="MNKRFSAGELAFRDEVRAFLRDQLPEDISHKVGNDLQLEREDYV
                     RWQKILHARGWAGVNWPPEYGGTGWTPVQKYIFATECAFAHTPRIVPFGLAMVGPVIY
                     TFGNAQQKARFLPDILASNVWWCQGYSEPNAGSDLASLRTSAVRDGEHYVVNGTKTWN
                     TMGQWADWIFCLVRTGGPELKPQAGISFLLIDMKTPGITVSPIRLLDGTPEVNEVHFD
                     HARVPAANLVGEENRGWTYAKYLLTHERTGIAQVAKSRMRLAKLKEIARGTAHEGAAL
                     IEEPCFARRVAEVEIDLMALEYTELRALASVSTGKAPGPESSILKIKGTEIAQAIDEL
                     LVEAAGWYALPFVPEQFHAGYEGWRVGPGAAANAAPIYFNNRKASIYGGSNEIQRNII
                     AREVLGL"
     gene            complement(12489..12875)
                     /locus_tag="PLW13_08080"
     CDS             complement(12489..12875)
                     /locus_tag="PLW13_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HQY70370.1"
                     /translation="MEHSSRALRAVFVGLGMTALALGIAGIVLPVLPTTPFVLLAAAC
                     FARGSTKFHDWLLGHPLFGPMIVEWQEYGSIPWRTKIVAIALMSTTLGVSIVFFVEPR
                     WLKAALTVLGLVLATWLYRVPSRDRL"
     gene            complement(12881..13447)
                     /locus_tag="PLW13_08085"
     CDS             complement(12881..13447)
                     /locus_tag="PLW13_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HQY70371.1"
                     /translation="MALKSTIHRLELDVADIDHGNYGSFSLTLARHPSETGERLMMRV
                     LAFALHADPALEFGRGISSEDEPDLWLKDVTGAIRMWIDVGLPDERRLRRAAGRAERI
                     VVIAYGGRAVDVWWEKGREALQRLPALTVLAIPFEAGREMAAFVDRSMRLQCTVQEQQ
                     VYLNDDARSVLIEPALLHERGAGQPESS"
     gene            complement(13590..>14740)
                     /locus_tag="PLW13_08090"
     CDS             complement(13590..>14740)
                     /locus_tag="PLW13_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HQY70372.1"
                     /translation="RPGGALLTHPAVMGAGPDDNPKGAFARRATRVSGYPEFGGEFPV
                     AALAEEMLTPGAGQIRALVTVAGNPVLSVPNGQLTDRALAGLDFMLSVDIYLNETTRH
                     AHLILPPSSTLEHDHYDLAFHKFAVRNTARYNAPVLPKPAGALHDWEIFAGLAAAHAA
                     LSGEPAAPVVPPADMLDFLLQSGPYGARSGFAPPLSLATLQAHPHGIDLGPLRPSLTE
                     RLCTADRMIDCLPAAIPGEIERLAAELAVPAAADALLLIGRRHIRSNNSWMHNSHRLV
                     KGPRRDQLLMHPQDLAARGVRDGQRVTVSSRTGSVSVEVQASEAIMPGTVSLPHGWGH
                     DREGVRLGIAAQHAGVSANDLTDERCIDVASGNAALNGVPVRVCGAHD"
BASE COUNT         2363 a         5037 c         5078 g         2262 t
ORIGIN      
        1 cagcatctcc tgctgcagtt tctcatcgcg cgcgatcgat gcgatctcgc cccactccgc
       61 caccgggcat tgcccgtggc agatgccgaa gcgcctgccg ctgcgatgct ccaccgtgat
      121 cgcccacggc atccgtgcgc acagcgcgcc cgcgcggcgg cccgccgcgt tcggctccca
      181 gccgtccagc gcccagtcgc cgccgttctg gcgccagacg tcgagcagca atatctcgcc
      241 ggcgagtacg cgcagcagca tgtcttcgtg gttgccgcgt accgcgtaaa accacggttc
      301 gccgagcagc gctgcgcagc cgggcgagtc gggaccgcga tcgatcaggt cgcctgtgct
      361 gaacaggcga tcgcatccgg ggtcgaagcc cgcgcgcgcg agcgcatcct gcagcgcgcc
      421 caggtatccg tgcaggtcgc cgaccacgaa atcgcgaccg cgccggttcg gcggcaccac
      481 gcgatggaac gcgctcacgc gcccaccgcc tgcgccgcgc ttgcgccggg tgcgggcgcg
      541 gccgcgcgca ggtgccgccg gctggtgcgc gccaggcgcc aggcgatcgc caacgccgcc
      601 acgccgatcg cggccagcat gccgatccag aagcccgcga ccccgagccc ggcgctccac
      661 ggcgagccca tcgccagcgt ccagcacagc ggcagcccga acagccagta ggcggcgata
      721 ttgatggccg aggcgacgcg cgtgtccttg tagccgcgca gcgccatgcc ggccccgatc
      781 tgcaccgagt cgaacacgcg gtaggcggcg gcgaacagca acagcatcgt gccgagctcg
      841 gcgaccgcgg cgtcgcgggt gaacagcgac gtgatcgggt ggcgcagcgc gatgatcgcg
      901 ccggcctgca gcagcgcgat gcccacgagc agcagcatgc cggtggcgca gacgtcgcgc
      961 gcgccgcgcg gattgccgcc gcccagctcg tgcgccacgc gcaccgacat cgcctggccc
     1021 acgccgagcg ggatcgtcag catcagggta tcgacggtga tggccaccgc gtgcccggcg
     1081 atcgcgaccg cgccgagcga ggccatcagc atcggcaaca ccgcgaagaa accgccgacg
     1141 gcgagcagcg acaggccgat cggcagcccg agcgcgagca tggcgcgcag ctcgcgcacg
     1201 attggagcgc cgaagccttg ccacaagcgg taaccgcgca gcgccggcgc aaacagcgcg
     1261 tacagggtcc acgcgaagac gagcagcgcg aaggagatca tggttgccca accgcagccg
     1321 gcgccgccga ggcgcggcag gccgaaggcg cccagcacca gtgcgtagtc gagcgggatg
     1381 ttgatcacga aggcgcccag gctgagccac atgacgggcc gcgtcaggcc gaggccttcg
     1441 ttggtggtgc gaaagcagat cgcgacgggc agcagcagcg ccgtgcctat ggtcggcaac
     1501 agatagcctg ccgctaccct ggtgacttgc ggctccgcgt cgagcaggcg taccgcgtgc
     1561 atggcgccga agatcgcgag ggccgacagc acgccgagcg gcaacgccag ccacatggcc
     1621 tgctggaact ggcggcggat ggccgcgacg ttgccggcac cgtacgcatg accgatcaac
     1681 gcgctgttgg cgagcatcgt gccgaagacc agcagcatcg acaggtccca ggcgtagaag
     1741 ccgatggtga cgccggcgag gtcattggtg ccggcatgcc cggcgaccac cacgtcggtg
     1801 acccccatgc cgatgatcgc gaactgtccc gccaccagtg gcagcgcgag ctgcagcatg
     1861 cggcgcgatt cggcgcgaaa tcgctgcaga cggggcggat tgcgggagtt gctgcctgtc
     1921 atcgggacct gcgtgtcagg cgtgttgtcc gtgcaacgga tcctggcgat agaagcgcgc
     1981 gagcagcgcg taggcctcgg gcagcacctc ctgcaggtgc tccgggatcg tgaagaagtg
     2041 ttcgctgagc accgcgaaga actccgcggg agattccagc gcgtagtcgt ccacctcggt
     2101 ctcgccgtag gcgtcccagt ccgctccggt gcgctcccac gcggcggtca tcacgcgcct
     2161 ccactcggcg ccactgacgc ccgatcccga caggtcgggc acaccgttca tgtcgccgtc
     2221 cccgagatcg agcttgtgcg cgcactcgtg gatcacgacg tggtagccgt cggcctcgcg
     2281 cgaggcctcc acgtccgccc acgacagcag catcggcccg gcatcccagc tctcgcccgc
     2341 gacgcgctcg tgacccgtgt gcaccacgcc ggcttcgtcg atttcctcgc gcggcgcgag
     2401 gaactcgtcc ggatacacga tcacgccgcg aaagctgcga taggcgccga ggccgaggtg
     2461 cagcaccggc agcgcggcgg cgatggcgat ctcgatgcgt tgccgtggct gcaggctgaa
     2521 accggccgct ggcgcgacga tcttgtcggc gaggaacagt gtcgcggtct cgcgcaggcg
     2581 ctggcgcccg gcagcgtcca gccgcgctag cagcggcagc cgcgcgcaga cggcctccca
     2641 gtcgcgatcg gcaaaggcat gacgggcaag ttttcgacgg cgccagaact cgaacacgcg
     2701 gactccggtg acaaccgggt cagcgtacca cggtaacggc gcaatcggcg tggcggatga
     2761 ccttctcgct gacactgccg agcagcagtt cgcgcaccgg cgacagcccg cggctgccca
     2821 tcacgatcag gctggcccgc tcgcggcgcg cgagcgcgac gatttcctcg gccgggtcgc
     2881 cgatgctgac cagcgtgcgt gcgacagccg cggggccgat cgctgcgcgc gccttgtcga
     2941 agctcggtcc cgcgatgcgc cgctcggcct cctcgatctg tgccgcatcg agcgcgccca
     3001 gcgccagcgt ctccgagccg cgcgcagcgt gcacgtgcag caggacgagt tgcgccccgc
     3061 gaccggccgc atacgtcgcg gcccacgccg cggcgcgcgc cgatgcatcg gacccgtcaa
     3121 ccggtaccag cacggtttcc atcgttcgcg gtctcccttc gtcggttcgc tcagagcagc
     3181 ggggcgatca ccaggctcac gatcgccatc acgttgatca ggatgttcat cgaggggccg
     3241 gcggtgtcct tgaagggatc gccgacggtg tcgccgacca ccgtggccgc gtgcacgggt
     3301 gagtgcttgc cgccgtagtg gcccttctcg acgtgcttct tggcgttgtc ccacgccccg
     3361 ccggcgttgg acatcatcag ggccagcagc acgcaggcga gcagcccgcc gccgagcgca
     3421 ccgcccagcg cctcggcgcc gatgccgaaa cccaccagcg gcggcgtggc gacggcgatg
     3481 agtccgggca gcagcatgcg ccgcagcgcc gcggccgtgg cgatgtcgat gcacctggcg
     3541 gtgtctggct ccgcggtgcc ttccatcaag cccgggatct cgcggaactg gcgccggatc
     3601 tcgacgatca tctccatggc ggcatcaccg accgcggtca tcgccatcga ggcgatcagg
     3661 aaggggacga tgccgccgat gaacagcccg accagcacgt gcgggttgtt cagcgacagg
     3721 tcgatcgtgc cctgtcccgt cgcggcccag tgcgcgtcga cggtctcgac gtaggccgcg
     3781 atgatcgcaa gtgccgccag tgccgcggca ccgatcgcga agcccttgcc tatcgcggcg
     3841 gtggtattgc ccagcgcgtc cagcgaatcg gtgatttcgc gcgtctcggg gccgaggccc
     3901 gccatctcgg cgatgccgcc ggcgttgtcc gccaccggcc cgaatgcgtc tattgccatc
     3961 gtgataccca ccgtcgccag catgccgacc gcggcgatcc cgaccccgta gagatccgcg
     4021 aacccggtcg acacataaat gatcgcgcag atcgcgagca tcggcacggt caccgactgc
     4081 atgccgaccg caagcccggt gatcagcacc gtcgccggcc cggtctgccc ggccgcggcg
     4141 atgcgcagca cgggcgtgga cgaggtgtac cactcggtga ccagcccgat cacgatgccg
     4201 ccggcggcgc cgcagatcac cgaccaccac accgcggcgt cgatgccgag cgcgcggatc
     4261 aggaaccacg ccgcgacgat gaacagcagc ggtgcggcta tggtgccgct gcgcagcgcc
     4321 agcgcgggcc gctgcgcgca caggaaccgc accaccacga tgccggtgac ggaggccagc
     4381 aagccggtgg acgcgagcgc gagcggcagg aacatcagcg ccgcgcgcgc gggcgcctcg
     4441 tccagttgcg gcgccagcat gccgagttgc ccgaaggcca tggtcgatgc gatcgcgatc
     4501 gtggcgatca tggcgccgca gtaggactcg aagatgtccg agcccatgcc ggccacgtcg
     4561 ccgacgttgt cgccgacgtt gtccgcgata acgccgggat tgcgcgggtc atcctccggg
     4621 atgcccgctt ccagcttgcc cacgaggtcc gagccgacgt cggcgctctt ggtgtagatg
     4681 ccgccgccga cgcgcgagaa cagcgcgacg gtagacgctc ccatgccgaa gccgtggatc
     4741 gcatgcgccg tgtgcgggtc gccgccgaac accaggtaca gcgaaccgag gccgagcagc
     4801 cccagcgagg cgacgcagag ccccatgacc gatccgccgt agaaggccac cgacagggcg
     4861 gccgccgcac cctgcgtgtg cgcggccgtg gtggtgcgca cgttcgcccg ggtcgcggcg
     4921 tacatcccga gccatcccgc tagcgccgag caggcggcgc cacagaggaa cgcgagcgcg
     4981 gtattggcgc cgaggaagta ccacagcagc gcgatcagta ccgcggcgaa cagcgccagc
     5041 gttttgtact cgcgctgcag gaacaccatg gcgccgagat ggatcgccgc ggctatcttg
     5101 cgcaccttgc cctcgccttc gggacggcgt gtgagcaaca ggtagatgat cagggcacag
     5161 gcaaggccga gggcgccgag caggggcggg agctgctgca tgccgggcat ggaatcctcc
     5221 ttgcgctggc gcaggacgaa tcagagctac agagccggct cgatcatagg agaggcgggg
     5281 ggcggaactc aagccccggt gcgtggcgcc tgaacacttt ctgtgaggac gtttccgcgg
     5341 aaacgtcgcg tggacttgtt cgatcgcccg ctatatattc ggtcgcgctg gcagggaagg
     5401 actccgaccg cgctctacac ggagacagag catgccaccg aaaattcgtg aactcatcct
     5461 gcagttgcag cgtgccggct ttgcggaccg gggcggaaag ggtagccacc gtaacttcgt
     5521 gcatccggat gcttcccgcc cggtgacctt gtccggcaac ccgggcgacg atgcgaggca
     5581 gtaccaggta cgcgccgtgc gcgcagctat cgaggagatc gagcgatgaa ggacgcagcc
     5641 cggtacgtaa aggtcgtgga gtggtccgaa gaggaccaat gctttgtcgg cagcgcgccc
     5701 ggactcgtgt ttggaggctg tcacggtgat gatgaaatgg cggtttttgc cgagctttgc
     5761 ctgatcgtcg acgaggcgat tctcctgtac aggcgggagg gaaagccgtt gccgccgccg
     5821 acgtccgggc gtgattttgt caacaagatg cagtgcgtgg cctagcagca catttcccgc
     5881 ttgcgccgtg cccattgaag tccggggtcg tggggaaagg cgccgacgtt tccgcggaaa
     5941 cgtcggcgct tcggtggaac gggagcaagg ttggatcagc gctatagttc ctcgaaatag
     6001 tccgagtcaa ttttcttgat gacatatgtc gccgaggcgg atacaccgac atcgaaatct
     6061 atcatcaggc tcgaaagcat attcccatcg atcagaacca ccttcgtatc gatcttgcca
     6121 acgtaatcga tggcatccga ggtgaaggac gacgcagtta tgaaaacacc ctttcgcgcc
     6181 cgctgtcctt gcaacgcgcc aacaaatttc tgtatctcgg ggcggccgac gtttccttgc
     6241 cagcgctttg cctgaatgta gatcacgtcc agtccgagtt tgtcttcctt gatgatgcca
     6301 tcgatacccc cgtcaccgct ttggccaatc cgttcgcccg cgtcggcccg cgagccgccg
     6361 taacccattt tcaccaacaa ttcgactacg aggttctcga aaaatgtcgg ggagcaggaa
     6421 atgatcctgg aaagcacttc ctgtgcgatc tccttccgca aatcctgata ggcgagctcc
     6481 agggtttcct ctggtgtaat ttctgccgga ctggcgttgt ttggggatga atagctcccg
     6541 tcattcttcc cggattccct gaatgccacg aactccggaa actgacccag aaatttgacg
     6601 tcgattcgac tgggcttctg cgcaattacg tttaacccgc gttcggtaat tcgaaacagt
     6661 ccacgcttcg gcgagtcgag gaggcctgcc tttttcatgt aagaccgtgc ccaaccgatg
     6721 cgattgtgga atattttctg agcaccgctc gggagaagag cggtccgctc agaaggcgtc
     6781 aagccaaata gatccgaaag tgcctctact gcttcgctga ccttgtggtc ctgttggtcg
     6841 ctggcgaact cgagaagcgg aagcatgcac gattgatagt ctggtatggc catgcaaatc
     6901 aatccttgtt ctcggcggat ctcaaaatcc cgagtgcttc tgcatgcaac gcccggttcg
     6961 ccgccgcgac cacgaagccg ccgcccgatg catcttcgcc gtggcgaccg ctgaccaccc
     7021 cgccggcggc ctcgatgatc gggatcagcg gctggatgtc gtaggcttgc aggccgccct
     7081 cgatcacgag gtcgatgtgc ccgagcgcca gcaggcagta ggaatagcag tcgccgccgt
     7141 agcgcatcag gcgcgtgtgc gccgcgacgt gctcgaaggc ggcgagctcc ccggcgctcg
     7201 ggaagatcgt gggatgcgtg cagtagagga tcgcgtcgac gaggcgcgcg ctggctcgcg
     7261 tctgcaggcg gcggcgctcg ccacggcgcg acagccaccc cccggccggc gagcccgtga
     7321 aggtttcctc gagatagggc tggtgcatca gcccgccgat gcagcgcccg gcgtcgcgca
     7381 gccccagcag gatgccccag gcgggcacgc cgctgatgaa ggcgcgcgtg ccgtcgatgg
     7441 gatcgatgat ccagctgtag cgcgagtcgc ctgccacggc gccgtgctcc tcgccgacga
     7501 tgccgtggtc ggggtagcgc gcgcggatgc gctcgagcag gaaggattcg acctcgcggt
     7561 cggcgatcgt gaccggatcg aagcggccgt cggcgagctt gttcgccacg tccagcggct
     7621 ggcggaaatg gcgcagcgcg atgcgcccgg cctcctgcac cagctcctcg gcaaaggcca
     7681 gcgcggcgcc ggcgtcggtg tggttcacgc ggaaatctcc ttcggttcgc gcagcgcgcc
     7741 gcgccagagc agccactgcg ccaggtaata gctgagcatg atcacgagcc cggcctgcgg
     7801 caccgggctg acgaacttgt tgatcgcgat cgtggcgtcg gagaaggcga acgccagcgc
     7861 accggcgagc agcagcaatg gacggttggc gagggccgcg cccgtgacca ttgcggtgat
     7921 cgcgagcatg tagagcgcga ccggcacggc catctcgccg gcgacgggca gcaccagcca
     7981 cgccagcccc gcggccaccg gcaggtaaca ggccgccagc cagcgccggc ggcgatctgg
     8041 cgcgcgttgg cgccagaaca gctgcgcata ggcgagctgc gcgacgagga agctgccgag
     8101 gcccgccgcg aacaggttgc ccaccacgcc gttcatgccc agcagccagt cgccgagcgc
     8161 cgagaaaagc agcgcgacca ccatccagcg ccgccaggtg ccgcgcatga gccgcgcgct
     8221 cgccgcggcg agcagcagga tcggcagcat cttcacgatg gcgccggccc agctgtcgtt
     8281 gtagccgaac aacagcaggt agacggccgc cagcagtgcg taggccgcgc tgatgccggc
     8341 gatcacgcct tcatcgcttg cgcgagcatg ggactcacac gcttgtccca cggtgcggcg
     8401 cgctcgatct gcgcggccag gcggatcagc gtcttctcgt cgccgtagcg cgccgcgaaa
     8461 tgcgtgccca ccggaatgcc cgccgcgttc tcgtgcagcg gcagcgtcat cgcgggctgg
     8521 ccggtggcgt tgaagatcgc gctgaagtcg ctgtagccca ccgccttctt gtagaactcc
     8581 tcggtcggca tcaccagcgt gacgtcgtcg accttccacg gcgcgcgcgg cgccatgggc
     8641 atcagcagca cgtcgatgtc ctgcatgaag gtgttcatcg tgaagcccag gcgctgcacg
     8701 gactggcgcg cggcctcgta gtcgagcgcg ctgatcgacg gcgcctgccg gtaatagccc
     8761 agcaccacgg gttccaggtc gtccggaccg ggttcgcggc cgagctcgcg tgcccgttcg
     8821 cggatctgtg cggacacccc caccatgctg aggaagccga agctctccat cagcttggag
     8881 aagggttccg gcagctcggc ttcgcgtacc tcgtggccga gggagcggca gagatcggcg
     8941 gccttcgcga tcgcatcgag gttgtcctgg tgcacggccg cacccgtcgg cgcgcgcgtg
     9001 atcagcgcga tgcgcagctt gccgggctcg cgcgccagtt cgttcatgta gctgtcggcg
     9061 ggcgcgggtg gccagtaggc cgcacccggc tcgggaccct gcgtcaggtc gagcagcagc
     9121 gcggtgtcgc gcaccgtgcg cgagaccacg tgaccgctcg agagccccga ccagccttcg
     9181 ctcttgtccg ggccgagcgg cgtgcgcgcg cgcgtgggct tcaggccgac gagtccgcac
     9241 atcgcggcgg ggaagcggat cgagccgccg ccgtcggtgg cgtgcgccgc cggcagtatg
     9301 cccgcggcca ccgcggcggc cgcgccaccc gagctgccgc cggcggagta ctcgaggttc
     9361 cacgggttgc gcgtcttgcc ccacagcgtc gagacggtgt tgggaatctg gccgaactcc
     9421 ggcgaagccg acttgccgaa gatgttcagg cccgcggcct ggtaacgctg taccacggtg
     9481 ctggtctggg tggcgacgcg atcggcgaag aagcgcgaac cgttgctggt gatggtgccc
     9541 ttcagataca ccccgaggtc cttcagcagg aatggcacgc cggcgagcgg cgcgttgggg
     9601 tcgatggact tcagcgccgc acgcgcctcg tcgtaatgct tcaggcagac cgcggtgatg
     9661 ctgccgttca ccgcctcggt gcgcgcgatc gcgagctcga gcagctcggc ggccgagacc
     9721 tcgccgcgtt tgaccagcgc cgcgagaccc atggcgtcgt gcgccacgta gtcgcggatc
     9781 gccatcttcg aggtgccggc ggcggaggcc ccgggcggga acagcgcggc gcctgctgcc
     9841 agcgtcgcgg cgccctgcac gaagtcgcgg cgggtcaggg tgttgtgctt atcggtcatg
     9901 agatgctcca aaacgttgca ggtgaaaggc ggtgttgccg aacagggtct cgatcgccgt
     9961 gaggcgcttg aagtagtggc cgacatcgag ctcgtcggtg acgccgatgc cgccgtgcag
    10021 ctggatcgct tcctgcccga cgcggcgcat cgcgcgaccg atgcggctct tcgccgccga
    10081 caccgggcgc gtgagcgcct cgccggcctc ggccgccatc agcgccatca tcaggatcga
    10141 gcgcgattgc tccagctcga tgaacatctc cgccatgcgg tgctgaagcg cctggaagga
    10201 ggcgatcgcc acgccgaatt gcctgcgcgt cttcgtgtac tccacggtct tgcccagcag
    10261 ggcgtccatc gcgcccaccg cctcggcgca gacgcagagc gtggcgcgct cgatcacgct
    10321 cgccagcgcg gcgagcccga tcgcggcggt gccgagcagc gcgtcggccg gcacgcgcac
    10381 ggcgtcgagt tcgatgtcgg cggcgcgccc gccgtcgatc gtcgcgtagc cgtgcatccg
    10441 caggccggcg ctggcgggcg cgacccggaa gatgccgatc gcgccaccgg catcgcgcgc
    10501 gctgaccagc agggtgtcgg cattcggcgc gttcagtacc acgcacttgt gcccgtcgag
    10561 tatccagccc tcgtcgttgc gcccggcggt ggtcgcgatg cgcgccaggc tgtagcggct
    10621 gccgggctcg gcgaaggcga gcgccagctg catctcgccg gccatgatcg cgggcagcat
    10681 cgactcgcgc tgcacgaggc tgccgagctc ggacaccagg cctgcgccca gcaccacggt
    10741 ggcgagcacg ggttccacga ccaggcccct gccgaactgc tcgaacagga tggcgagatc
    10801 cgtgaggttg ccgccgagtc cgccgtcatc ctcggccacc ggcaacgcga gccagccgag
    10861 ctcggcgaac agttgccagt gctgtgcgga aaatccgcgc gcgcccgcca cgatggcgcg
    10921 gcgcgcatcg aatgtgtagt gctcgcgcac gaagcgttcc acgctgtcgc gcagcagttg
    10981 ctgttcctcg ctgtattcga agtccatcgc ctgttgcctc cttcagccgt tggcacccac
    11041 gcctagcggg cgggcttgcc ttggcgcggc gggaccgtct gtcgcctgga tggcgacagt
    11101 cgagcccaca gggatgtgtt cacggcgtgt cccgacgggc caaggcaagc ctgcccgcgt
    11161 gctacggagc acagcaaatg ccatcacagc ccgagcacct ctctggcgat gatgttgcgc
    11221 tggatctcgt tcgaaccacc gtagatcgag gccttgcggt tgttgaaata gatcggcgcg
    11281 gcattggccg cggcgcccgg gccgacgcgc cagccctcgt agccggcgtg gaactgttcg
    11341 ggcacgaagg gcagggcata ccagccggcg gcctcgacca gcagctcgtc gatggcctgc
    11401 gcgatctcgg tacccttgat cttcaggatc gaggactccg gtcccggtgc cttgccggtg
    11461 ctgacgctcg ccagcgcgcg cagctcggtg tattcgagcg ccatcaggtc gatttccacc
    11521 tcggccacgc gacgagcaaa gcaaggctcc tcgatcagcg ctgcgccctc gtgcgcggtg
    11581 ccgcgcgcga tctccttgag ctttgccaga cgcatgcgcg atttggccac ctgcgcgatg
    11641 ccggtgcgct cgtgcgtcag gagatacttg gcgtaggtcc agccgcggtt ctcctcgccg
    11701 accaggttcg ccgcgggcac gcgcgcgtgg tcgaagtgca cctcgttcac ctcgggggtg
    11761 ccgtcgagca ggcggatcgg cgagaccgtg atgcccggcg tcttcatgtc gatcagcagg
    11821 aagctgatgc cggcctgcgg cttcagttcc gggccgccgg tgcgcaccag gcagaagatc
    11881 cagtcggccc actggcccat ggtgttccag gtcttggtgc cgttcaccac gtagtgctcg
    11941 ccatcgcgca ccgcgctggt gcgcagcgag gcgaggtccg atcccgcgtt cggctcggag
    12001 tagccctggc accaccagac attgctggcg aggatgtcgg gcaggaagcg cgctttctgt
    12061 tgcgcgttgc cgaaggtgta gatgacggga ccgaccatcg cgaggccgaa gggcacgatg
    12121 cgcggtgtgt gcgcgaaagc gcattctgtg gcgaagatgt acttctgcac cggcgtccag
    12181 ccggtacccc cgtattcggg tggccagttg acgccggccc agccgcgcgc gtgcagtatc
    12241 ttctgccagc gcacgtagtc ctcgcgctcg agctgcagat cgttcccgac cttgtggctg
    12301 atatcctcgg gaagctggtc gcgcaggaag gcgcggacct cgtcacggaa agcgagctcc
    12361 ccggcgctga atcgcttgtt catgtgcttc ctggccccgg cgcggtgcag cgcatattcg
    12421 ccgcacgccg gcggcagatc aagtaggagc gcgccatgcg cgcgaacggt cgcggccatg
    12481 ggccgctcct acaaccggtc gcgcgagggc acgcgataga gccaggtggc gagcaccagt
    12541 cccagcaccg tcagcgcggc cttcagccat cgcggttcca cgaagaacac gatcgagacg
    12601 ccgagcgtgg tgctcatcag cgcgatcgcc acgatcttgg tgcgccacgg gatgctgccg
    12661 tactcctgcc actcgacgat catcggcccg aacagagggt gccccagcag ccagtcgtgg
    12721 aacttcgtcg agccgcgcgc gaagcaggcc gcggccagca gcacgaaggg cgtggtcggc
    12781 agcaccggca gcacgatgcc ggcgatgccg agcgcgagcg cggtcatgcc caggccgacg
    12841 aagacggcgc gcagtgcgcg cgagctgtgt tccaccacgc tcaggacgac tctggctggc
    12901 cagcgccgcg ctcgtgcagc agcgccggct cgatcagtac cgagcgcgcg tcgtcgttca
    12961 ggtagacctg ctgctcctgc accgtgcact gcaggcgcat cgagcgatcg acgaacgcgg
    13021 ccatctcgcg tcccgcctcg aaggggatcg cgagcaccgt gagcgcgggc agccgttgca
    13081 gcgcctcgcg gcccttctcc caccacacat cgaccgcgcg cccgccgtag gcgatcacca
    13141 cgatgcgctc ggcgcggccc gcggcacggc gcaggcgccg ctcgtcgggc agtccgacgt
    13201 cgatccacat cctgatcgca ccggtgacgt ccttcagcca caggtccggt tcatcctcgc
    13261 tcgagatgcc gcgcccgaat tcgagcgccg ggtcggcgtg cagcgcgaag gccagcacgc
    13321 gcatcatcag ccgctccccg gtctccgagg ggtggcgcgc cagcgtcagc gagaagctgc
    13381 cgtagttgcc gtggtcgatg tcggcgacat cgagctcgag gcggtggatc gtggacttca
    13441 gcgccatgcg cgtgccgtct gctgatgctg cggtgaggtc atggtcgcgg agctttcctg
    13501 cggcgggtgg gcgaaacggt ggatggcgct cgagggcgct atggtagcgg cgcgcgggcg
    13561 tgcccggaag gcgtgcgcgc agcgccggat cagtcgtgcg cgccacacac cctgaccggc
    13621 acgccgttca gcgcggcatt gcccgaagcg acatcgatgc agcgctcgtc ggtgaggtcg
    13681 ttggcgctga cgccggcgtg ctgcgccgcg atgccgagcc taacgccctc gcggtcgtgg
    13741 ccccagccgt gcggcagcga caccgtgccc ggcatgatcg cctccgaggc ctggacttcg
    13801 acgctgacgc tgccggtgcg cgagctgacg gtgacgcgct ggccgtcgcg cactccgcgc
    13861 gccgccagat cctgcgggtg cataagcaac tggtcgcggc gcggcccttt gaccagccgg
    13921 tggctgttgt gcatccacga gttgttgctg cggatgtggc ggcgcccgat cagcagcagc
    13981 gcatcggctg cggctggcac tgcgagttcg gcggcgagac gctcgatctc gccgggaatc
    14041 gcggcgggca ggcagtcgat catccggtcc gcggtgcaca gtcgctccgt cagcgacggc
    14101 cgcagcggcc cgaggtcgat gccgtgcggg tgcgcctgca gcgtggcgag gctgagcggc
    14161 ggcgcgaagc ccgagcgcgc gccgtagggc ccggactgga gcaggaagtc gagcatatcg
    14221 gccggcggca ccaccggcgc ggccggctcg ccgctcagcg cggcgtgggc tgcggcgagc
    14281 ccggcgaaga tttcccagtc gtgcagcgcg ccggcgggtt tgggcagcac cggcgcgttg
    14341 tagcgcgcgg tgttgcgcac cgcgaacttg tggaatgcga ggtcgtaatg gtcgtgttcc
    14401 agcgtcgagc tcggcggcag gatcaggtgc gcgtggcgcg tggtctcgtt caggtagatg
    14461 tccacgctca gcatgaagtc gagccccgcc agtgcgcggt cggtcagctg tccgttcggc
    14521 accgagagca ccgggttgcc ggccaccgtc accagcgcgc ggatctgtcc cgcgcccggc
    14581 gtcagcatct cctcggcgag cgctgcgacc gggaactcgc cgccgaactc gggatagccg
    14641 ctgacccggg tcgcgcggcg cgcgaaggca cccttggggt tgtcgtccgg tccggcgccc
    14701 atcacggcgg ggtgggtgag cagcgcgccg cccgggcggt
//
LOCUS       DAORUQ010000072        14607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3951_length_14607_cov_6.478560, whole genome shotgun sequence.
ACCESSION   DAORUQ010000072 DAORUQ010000000
VERSION     DAORUQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3951_length_14607_cov_6.478560"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..465
                     /locus_tag="PLW13_08095"
     CDS             85..465
                     /locus_tag="PLW13_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70373.1"
                     /translation="MNTNAYRPLARGARAARSVSRMRALACALIVLPVAVAGAAANTN
                     PQRESPVEECVQAAVARMGSELTQRIEGSVRMFGDAQAMQVVYLDIASDGARLPVRLR
                     LYCSVNERGEIEHVLSMPRLLRAG"
     gene            complement(525..1706)
                     /locus_tag="PLW13_08100"
     CDS             complement(525..1706)
                     /locus_tag="PLW13_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY70374.1"
                     /translation="MDQRVVFLLSLAAFTSAASLRATDPLLALIAGEFGVTAGKAGAV
                     ITGFALSYGVFQLAHGPIGDRFGKFRIITVMTAVSAVATFGCALAPTLGTLTLMRFLA
                     GVTVGAIIPLAMAWIGDTVAYERRQATIARFMIGQMLGGAAGVSLAGALAAWLGWRAI
                     FVVLGALFIVIAALLRRELARNEPLRLDGDRSVTIANALRRMAGLLLRPWARVILAVV
                     AIECALSMGVFAYVAWDLQHRHGVSLALSGFWIVAYPAGGLLYATFSAPLVARLGEQG
                     LALAGGLLIGAGYLCLLLAPAARVVLPGLVAVGLGFAMLHNTLQVNATQMAPEARGAA
                     VSLFAFCLFTSQSAGIAIVGRVIDAAGIWPAYLAAALGLPLLALEFRRRLRHKTAGCA
                     A"
     gene            1840..3210
                     /gene="dbpA"
                     /locus_tag="PLW13_08105"
     CDS             1840..3210
                     /gene="dbpA"
                     /locus_tag="PLW13_08105"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HQY70375.1"
                     /translation="MSKFTDLNLSPALLQAVDALGYTDMTPIQAGALPLLLEGRDVLA
                     QARTGSGKTAAFALALLAKLHCERSRLQVLVLCPTRELADQVSKEIRALARFIPNVKV
                     LTLCGGVPLRPQLASLAHEPHIAVGTPGRIGELIEREALRLDSVRCVVLDEADRMLDM
                     GFLDAVRGILAGTPAARNTWLFSATWPAEIRALSAQFQDAPVTVAIEDAHEQAAIEQR
                     FYQVEAADKPDAVIALLLEHQPASCLVFCNTKVDVKTLSEALCKRGVSALALHGDLEQ
                     RERDETLLQFANGSARVLVATDVAARGLDIKALPMVISWELAHDADTHVHRVGRTGRA
                     GESGLALSLVASRETGRVARIEEIIGAPIAWAKLPSRGHSLSMPAPAMRTLAIDAGRQ
                     DKLRAGDILGALTGEAGLQAAEIGKIDLFATRAYVAISRARFDAALQQLRNGRIKGRN
                     FRVRGL"
     gene            3216..3929
                     /locus_tag="PLW13_08110"
     CDS             3216..3929
                     /locus_tag="PLW13_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY70376.1"
                     /translation="MRLNKFIGETGLCSRREADELIAAGRVTLDGSVAVAGAKWAEGM
                     VVCVDGKEIRRKPAAKNRRRHVYIALNKPVGITCTTDTADASNIVDFIDHQERIFPIG
                     RLDKDSEGLILLTSNGDIVNRILRVENNHEKEYLVGVNKPVTDEFLRTMARGVHIHRQ
                     LTKPCKVSRLAKYGFRITLTQGLNRQIRLMSEALGYKVVQLRRVRVINVRLGTLKPGA
                     WRNLSDAELAGLLPDFKDW"
     gene            4042..5628
                     /locus_tag="PLW13_08115"
     CDS             4042..5628
                     /locus_tag="PLW13_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HQY70377.1"
                     /translation="MIATSNLTIQFGARPLFENVSVKFGNGNRYGLIGANGCGKSTFM
                     KILGGDLEPSSGSVMRPVDVRLGKLRQDQFAYEQFSVLDTVIMGHEELWQVKAERDRI
                     YALAEMSEEEGMAVADLEVRFAELDGYTAEARAGELLLGLGIAVEQHAGPMSAVAPGW
                     KLRVLLAQALFSDPDVLLLDEPTNHLDINTIRWLEGVLKARNSTMVIISHDRHFLNSV
                     CTHMADLDYGALKLFPGNYDAYMTAATQARELLQNENAKKKAQIAELQSFVSRFSANA
                     SKAKQATSRARQIEKIELTEIKPSSRQSPFIRFDQKQKLHRQAVTLRDVSKGYGAAPL
                     FTNFSIQVDAGERVAIIGPNGAGKTTLLRCLMRELALDSGELKWAEAADIGYYAQDHA
                     ADFAEDMSLFDWMKQWTRGDEQLVRGTLGRMLFSQDEITKSVKVISGGEQGRMLFGKL
                     ILQRPNVMVLDEPTNHLDMESIESLNLALENYPGTLIFVSHDREFVSSLATRVLEFTS
                     AGIVDFSGTYDEYLRSQALE"
     assembly_gap    5722..5821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5979..6992)
                     /locus_tag="PLW13_08120"
     CDS             complement(5979..6992)
                     /locus_tag="PLW13_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY70378.1"
                     /translation="MQTEQWIEHELPLGDMPLQCGAVLRDTRIRYLQRGYPNAPKDNI
                     VLLPTYYGGAMAGNRALGDGASPLADPRWCLLIPAMLGAGESTSPSNAHPAQAGARFP
                     QITLADQVMAQARLVDMLFGDVTLALVAGWSLGGMQSLQWGCLLAQRVRRVAAWCSAA
                     RCHPHNLLFLRGLEAALLADPLSAGDDKPVAGLRAFGRVYASRAYGPRFVSEGHYHAL
                     GFQDIEALLGWWEADHLAMNHHDLLAVLRMWAAADIAANPRFAGDHTAALAAIGAPAL
                     VMPCATDQYFLVHEAEAEAACMPQADFRVMQSEWGHCAGGPGREPAAMAQLWQGLRDL
                     LAR"
     gene            7123..8481
                     /gene="glnT"
                     /locus_tag="PLW13_08125"
     CDS             7123..8481
                     /gene="glnT"
                     /locus_tag="PLW13_08125"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HQY70379.1"
                     /translation="MSAPHTDHALATRLGKAGARYAMASYVDIHGRIKGKMVPLGHLG
                     NMLAGSELYTGAALDGVPQEISDNEVAAMPDPASATQCPWNPDLVWFASDLYLDGKPF
                     EACSRVILKRQTAAAAALGYTFNLGIETEFFLFRDLPGGGFGPVSDRDNLGKPCYDPC
                     TLMDNLPIVDELVSAMNALGWDVYSFDHEDAIGQFETDFTYADALTMADRLVFFRMMA
                     NEIARKHGAFASFMPKPFADRTGSGAHYNMSLADRETGTNLFAPAGDDVHGVGVSKLA
                     YHFTAGVLRHARAISAVIAPTVNSYKRLVRQGSMSGSTWAPVFCCYGNNNRTNMIRVP
                     ACGSRIECRAADISCNPYLGAALILAAGLEGVREQLDPGLPHRENMYNYSDAEIAARN
                     IQFLPQSLEEAIDAFEADPLSREVFGPRMFDTFVQFKRAEWRSYHNHVSDWEIERYLK
                     MF"
     gene            8534..9607
                     /locus_tag="PLW13_08130"
     CDS             8534..9607
                     /locus_tag="PLW13_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY70380.1"
                     /translation="MRYPVLKRLAAVLLAGAAVVCAGGASAEEKQKFSIAWTIYAGWI
                     PWKYADESGIMKKWADKYGIDVRIVQVNDYVESINQYTAGQFDGVVATSMDTLSIPSA
                     SGVDTTALIVGDYSNGNDGIVSKQADSIAGFKGGKVHLVELSVSHYLLARALASVGLS
                     ERDVSIVNTSDADIVAAYATPEVTHAVTWNPLLDQVAATPGTRKIYDSSSIPGHIKDL
                     TVVNSATLADNPAFGKALVGAWFEMMAILESPGPEGQKMRAMLGEASGTDRAGYESQL
                     ARLKMFWKPADAVAFVKSAQAYEAMDSVRRFSFDHGLLGEGASDADFVGISFPDGKQL
                     GNAANTKLRFDTTYMQMAADGKL"
     gene            9653..10252
                     /locus_tag="PLW13_08135"
     CDS             9653..10252
                     /locus_tag="PLW13_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQY70381.1"
                     /translation="MRRTREEAEMTRRRVIEAALKLFSRNGYSQTTLAMIAAEAGYSR
                     GPIYWHFSNKDELFEAVIGHSQEPLLQLVERARLEPDPRRAIDDFVDEWFRLLVEDDW
                     FRQSFEILLNKTELTAAMVRTIRRERQLTRAIIATLGARIRELRPAGDVDAESLGLLC
                     YTYLMGITQSWLFAPKLFSLAGRRDFFRTHLHGMLGLSR"
     gene            complement(10242..11087)
                     /locus_tag="PLW13_08140"
     CDS             complement(10242..11087)
                     /locus_tag="PLW13_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY70382.1"
                     /translation="MSPRRSRLLLGFLVFGFAFLYVPILLLVIYSFNESRMVTLWGGF
                     STKWYAALLQDRAILDAAWKSLSLGFMSASASLVLGTAAGIALTRFGRYRGRNLLGGL
                     ITAPMVMPEVITGLSLLLLFVAMEQMIGWPAGRGMTTLWIAHVTFTTSYVAIVVSSRL
                     RELDLSIEEAALDLGATPLRVFFLITLPIIAPALVSGWLLAFTISLDDLVISAFVSGP
                     SSTTLPMVVFSSVRMGISPKINALATIIVGVVTAIVVLSGIWMMRQEKRRQREIAQAA
                     QEVTG"
     gene            complement(11087..11995)
                     /locus_tag="PLW13_08145"
     CDS             complement(11087..11995)
                     /locus_tag="PLW13_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY70383.1"
                     /translation="MKARRFGFGRFGIIMVPYVWLILFFLVPFGFVLKISFAEIANAI
                     PPFTSLIQYDADGHAHITLNFDNYRYLWEDELYYIAYLSSLKIAALSTLLCLLVGYPM
                     AYAIANSSESLRNVFLMLVVLPSWTSFLIRIYAWIGILKNEGLLNNLLLWLGLVEQPI
                     QILQTDVAVYIGIVYAYLPFMILPLYTNLAKHDYSLIEAAQDLGCSAWKAFLKITLPL
                     SQRGIIAGSMLVFIPAVGEFVIPELLGGPDTLMIGRVLWNEFNSNRDWPVASAVAAVM
                     LIVLIGPLLFHQHMQNKELDRQEGTG"
     gene            complement(11992..13122)
                     /gene="potA"
                     /locus_tag="PLW13_08150"
     CDS             complement(11992..13122)
                     /gene="potA"
                     /locus_tag="PLW13_08150"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HQY70384.1"
                     /translation="MLQQAASSTRAPAQDGYVRIENVSKSFDGVLAVNDVSLSIPKGE
                     LFALLGPSGCGKSTLLRMLAGFETPSAGRILIDGADMAGTPPYIRPTNMMFQSYALFP
                     HMSVRDNIAFGLKQDRLPADTVDKRVGELLELVDMKTYARRKPHQLSGGQRQRVALAR
                     SLAKRPKLLLLDEPMGALDKKLRERMQLELVDIIETVGVTCVMVTHDQEEAMTMASRV
                     AIMSEGRFVQVGAPREIYEYPNCRFSAEFIGLVNSFEGDLLENESDHVTVGCNDLPVH
                     IHVDHGISGPDGMRVWLAIRPEKIQIERVQPEQEYNWASGVVRDIAYFGDMSVYHVQL
                     PSGKKVLCTMANTRRRHEEEPPTWGDEVFVSWDGADSVLLTA"
     gene            complement(13181..14281)
                     /locus_tag="PLW13_08155"
     CDS             complement(13181..14281)
                     /locus_tag="PLW13_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY70385.1"
                     /translation="MKFWNGLAAAAAALLVCGPVAAADRKELHIYNWSDYIAEDTIRN
                     FEKETGIKVTYDVYDSNEVLQGKLSAGSTGYDIVVPSTNFLGKQIPAGTHQKLDRSKL
                     PNFKNLDPMMLKYVAEVDPGNQYAVPYLWGTVGIGINVDKVRAVLGEDVALDSWDLLF
                     NPEYMAKLASCGVSFLDSPSEVYPLALFHLGKNPNSLDEADYTGAANELLMKIRPHIR
                     KFSSSEYINSLADGEICVAMGFSGDIGIAKARAEEAGNGVNIRYIIPKEGTEIWFDSL
                     AIPADAPNPESAHVFIDYMLRPEVIAAVTDFVTYPNPNKAATALVDKSLSGDPLVYPP
                     QEQIDRMFGLSPKPPKVERVMTRAWTKFKTGR"
BASE COUNT         2536 a         4874 c         4797 g         2300 t
ORIGIN      
        1 gaaacgagtc gcgcgtacgc ttctctccag gcgtcgggga attccctccc gcgccccgga
       61 tgtcaaccga caggagaagc ccagatgaac acaaacgcat atcgaccgtt agcacgtggt
      121 gcccgcgcgg cccgcagcgt ctccaggatg cgcgccctgg cctgtgcact gatcgtgctg
      181 ccggtggccg ttgctggcgc cgcggcgaac accaacccgc agcgcgagtc gcccgttgaa
      241 gagtgcgtgc aggccgcggt ggcccggatg ggcagcgagc tcacgcagcg catcgagggc
      301 tcggtgcgca tgttcggtga cgcgcaggcg atgcaggtcg tgtatctcga catcgcttcc
      361 gacggggcac ggctgccggt acgcctgcgt ctgtattgca gcgtgaacga gcgcggagag
      421 atcgagcacg tgctgtcgat gccgcgcctg ctgcgggccg gctgacgcat cgccccggct
      481 tcggccgggg catcgacacc tgcgtcatgc aggcgcgctg tcgctcaggc ggcgcagccg
      541 gccgtcttgt gccgcagccg gcgccggaat tccagtgcca gcagcggcag ccccagcgcc
      601 gccgcgagat aggccggcca gatgcccgct gcgtcgatca cgcgcccgac tatcgcgatg
      661 ccggccgact ggctggtgaa caggcagaag gcgaacagcg acaccgccgc gccgcgcgcc
      721 tcgggcgcca tctgcgtggc attcacctgc agggtgttgt gcagcatcgc gaagccgagg
      781 cccaccgcca ccagccccgg cagcaccacc cgggccgcgg gggccagcag caggcagaga
      841 tagccggcgc cgatcagcag cccgcccgcg agtgccaggc cctgctcgcc gaggcgtgcc
      901 accagcggcg cggagaacgt ggcatacagc aggccgccgg cgggataggc cacgatccag
      961 aagccgctga gcgcgaggct gacgccgtgg cggtgctgca ggtcccaggc cacgtaggcg
     1021 aacacgccca tgctcagcgc gcattcgatc gcgaccaccg ccaggatcac ccgcgcccat
     1081 gggcgcagca gcaggcccgc catgcgccgc agggcattgg cgatcgtcac cgagcggtcg
     1141 ccatccaggc gcagcggctc gttgcgcgcc agttcccgcc gcagcagcgc ggcaatgacg
     1201 atgaacagcg cgccgagcac gacgaagatg gcccgccagc ccagccacgc ggcgagcgcg
     1261 cccgccagcg ataccccggc ggccccgccc agcatctggc cgatcatgaa gcgcgcgatc
     1321 gttgcctggc gccgttcgta ggccacggtg tcgccgatcc aggccatcgc cagcggaatg
     1381 atggcgccca ccgtgacgcc ggcgagaaag cgcatcaggg tgagcgtgcc cagcgtgggc
     1441 gccagcgcgc agccgaaggt ggccacggcc gatacggccg tcatcacggt gatgatgcgg
     1501 aacttaccga agcgatcacc gatcgggccg tgcgcgagct ggaacacgcc gtaagagagc
     1561 gcgaacccgg tgatcaccgc gccggccttg ccggccgtca cgccgaattc gccggcgatc
     1621 agcgcgagca gcggatcggt ggcgcgcagg ctcgctgcgc tggtgaacgc cgcgagggag
     1681 agcaggaaga cgacgcgctg gtccatgccg ggcgcagtat cggggattgt gccggtcgtg
     1741 gcgagcccga gcgcaccgct gcgccgcccg cccggacacg cgaaggatta cgcagcattg
     1801 gccggccggt ggcccgctat tgccgtgcga taatggcgca tgagcaaatt caccgacctg
     1861 aacctctcgc ctgccctgct gcaggccgtc gacgccctgg gctacaccga catgactccg
     1921 atccaggccg gcgcgctgcc gctgctgctg gaggggcgcg acgtgctggc gcaggcgcgc
     1981 acgggcagcg gcaagacggc ggccttcgcg ctggcgctgc tcgcgaagct gcactgcgag
     2041 cggagccgct tgcaggtcct ggtgctgtgt cccacgcgtg aactcgccga ccaggtgagc
     2101 aaggaaatcc gcgcgctcgc gcgcttcatc ccgaacgtca aggtgctcac gctgtgcggc
     2161 ggcgtgccgc tgcgcccgca actggcctcg cttgcgcacg agccgcatat cgcggtcggc
     2221 actcccgggc gcatcgggga gctgatcgag cgcgaggcgc tcaggctcga cagcgtgcgc
     2281 tgcgtcgtgc tcgacgaggc ggatcgcatg ctcgacatgg gcttcctcga cgccgtgcgc
     2341 ggcatcctcg ccgggacccc cgcggcacgc aacacctggt tgttctcggc gacctggccg
     2401 gcggagatcc gggccctcag cgcgcagttc caggatgcgc cggtcacggt cgcgatcgag
     2461 gacgcgcacg agcaggctgc gatcgagcag cgcttctacc aggtggaggc ggcggacaag
     2521 cccgatgccg tgattgccct gctgctcgag caccagccgg cgagttgcct ggtgttctgc
     2581 aataccaagg tcgacgtgaa gaccctgagc gaggcgttgt gcaagcgcgg cgtgtcggcg
     2641 ctggcgttgc acggcgatct cgagcagcgc gagcgcgacg aaaccctgct gcagttcgcc
     2701 aacggcagcg cgcgcgtgct ggtggccacc gacgtggccg cgcgcggcct cgacatcaag
     2761 gccttgccca tggtgatctc ctgggagctc gcgcacgacg ccgacaccca cgtgcaccgc
     2821 gtcgggcgca ccggccgcgc gggcgagtcg ggcctggccc tgagcctggt ggcgagccgc
     2881 gagacggggc gggtcgcgcg catcgaggag atcatcggcg cgccgatcgc ctgggcgaag
     2941 ctgcccagtc gcggccattc gctttccatg ccggcgccgg cgatgaggac ccttgccatc
     3001 gatgcgggcc gccaggacaa gttgcgcgcg ggcgatatcc tcggtgcgct gaccggcgag
     3061 gccggcctgc aggcggccga gataggcaag atcgatttgt tcgccacgcg cgcctatgtc
     3121 gcgatcagcc gcgcgcgctt cgacgcggcg ctgcagcagc tgcgcaacgg caggatcaag
     3181 ggccgcaatt tccgggtgcg cggcctgtga gcggcatgcg cctgaacaag ttcatcggcg
     3241 agaccgggct gtgctcgcgc cgcgaggccg acgagctgat cgccgccggg cgcgtgacgc
     3301 tggacggcag tgtcgcggtg gcgggcgcca agtgggccga gggcatggtg gtctgtgtcg
     3361 acggcaagga gatcaggcgc aagcccgcgg cgaagaaccg gcgccggcac gtctacatcg
     3421 cgctgaacaa accggtcggc atcacctgca ccaccgatac cgcggacgcg agcaacatcg
     3481 tcgacttcat cgaccaccag gaacgcattt tcccgatcgg gcgcctcgac aaggactccg
     3541 agggcctgat cctgctgacc agcaacggcg acatcgtcaa ccgcatcctg cgcgtggaga
     3601 acaaccacga gaaggaatac ctggtgggcg tgaacaagcc cgtcaccgac gagttcctgc
     3661 gcaccatggc gcgcggcgtg cacatccacc gccagctgac gaagccctgc aaggtctcca
     3721 ggctggcgaa gtacggcttt cgcatcacgc tgacgcaggg cctcaaccgc cagatccgcc
     3781 tgatgagcga ggcgctgggc tacaaggtgg tgcagttgcg ccgcgtgcgc gtgatcaacg
     3841 tgcgcctcgg cactctcaag cccggcgcct ggcgcaacct gagcgacgcc gagctggccg
     3901 gcctgctgcc cgatttcaag gactggtagc aggattcgcg cgcgatttcg caaatagtgc
     3961 aattgcggtt ttcgcgccag ccactacact ggccggcccc gcagagcgcc cgtgcgccgc
     4021 cgtcaactcc aggttatttc cgtgatcgcg accagcaacc tcactatcca gttcggggcc
     4081 cggcccctgt tcgagaacgt ttccgtgaag ttcggcaacg gcaaccgtta cggcctgatc
     4141 ggcgccaacg gctgcggcaa gtccaccttc atgaagatcc tcggcggcga cctcgagccc
     4201 agttcgggca gcgtgatgcg cccggtcgac gtgcgcctgg gcaagctgcg ccaggaccag
     4261 ttcgcctacg agcagttcag cgtgctggac acggtgatca tgggccacga ggagctgtgg
     4321 caggtcaagg ccgagcgcga ccgcatctac gcgctggccg agatgagcga ggaggagggc
     4381 atggcggtag cggacctcga ggtgcgcttc gcggagctcg acggctacac cgccgaggcg
     4441 cgcgccggcg aactgctgct aggcctcggc atcgcggtgg aacagcacgc gggcccgatg
     4501 agcgcggtcg cccccggctg gaagctgcgc gtgctgctgg cgcaggcgct gttctccgat
     4561 cccgacgtgc tgctgctcga cgagccgacc aaccacctcg acatcaacac catccgctgg
     4621 ctcgaaggcg tgctgaaggc gcgcaacagc accatggtga tcatctcgca cgatcgccac
     4681 ttcctgaaca gcgtctgcac gcacatggcg gatctcgatt acggtgcgct caagctgttc
     4741 cccggcaact acgacgccta catgaccgcc gccacgcagg cgcgcgagct gctccagaac
     4801 gaaaacgcga agaagaaggc gcagatcgcc gagctgcagt ccttcgtcag ccgcttctcc
     4861 gccaacgcgt ccaaggccaa gcaggccacc tcgcgcgcgc gccagatcga gaagatcgag
     4921 ctcaccgaga tcaagccctc gagccgccag agcccgttca tccgcttcga ccagaagcag
     4981 aaattgcatc gccaggcggt gacgctgcgc gacgtgagca aggggtacgg cgcggcgccg
     5041 ttgttcacga atttcagcat ccaggtggac gcgggcgagc gcgtcgcgat catcgggccc
     5101 aacggcgccg gcaagaccac gctgttgcgc tgcctgatgc gcgaactggc gctggacagc
     5161 ggcgagctga agtgggcgga ggcagccgat atcggctact acgcgcagga ccacgcggcg
     5221 gacttcgccg aggacatgag cctgttcgac tggatgaagc agtggaccag gggcgacgag
     5281 cagctggtgc gcggcacgct cgggcgcatg ctgttctcgc aggacgagat caccaagtcc
     5341 gtgaaggtga tttccggcgg cgagcagggt cgcatgctgt tcggcaagct gatcctgcag
     5401 cgccccaacg tgatggtgct cgacgagccc accaaccacc tcgacatgga atcgatcgag
     5461 tcgttgaatc tcgcgctgga gaactacccc ggcacgctga tcttcgtgag ccacgaccgc
     5521 gagttcgtgt cctcgctggc gacccgcgtc ctcgagttca cgtccgcggg catcgtcgat
     5581 ttcagcggca cctacgacga gtacctgcgc agccaggcgc tggagtagga gcgacgggcc
     5641 gtcccgccag ccgggacacg ctcccgttgg ccgcaaccgt gcacagcagc cggcgctggt
     5701 agacggcgcc atgcgcctcg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 ngagcgggca ggggagcagt tcctgacggt gttcatgacg cttctccgtg gggcaactct
     5881 gacgctgcga tcattgcccc cggctgttgc tggcgcattt cccgccgttt ccgccgtgtt
     5941 ggcggattgt tgccgccctg tgtcggcgca gggatccctc agcgcgccag caggtcccgc
     6001 agtccctgcc acagctgcgc catcgccgcg ggttcgcgcc cggggccgcc ggcgcaatgc
     6061 ccccattcgc tctgcatcac gcgaaaatcc gcctgcggca tgcatgccgc ctcggcctcg
     6121 gcctcgtgga ccaggaagta ctggtcggtg gcgcagggca tgaccagcgc cggcgcgccg
     6181 atggcggcca gcgccgccgt gtgatcgccc gcgaagcgcg ggttcgccgc gatatcggcc
     6241 gcggcccaca tgcgcagtac cgcgagcagg tcgtggtggt tcatcgcgag gtgatcggct
     6301 tcccaccagc cgagcagcgc ctcgatgtcc tggaagccga gcgcgtggta gtggccctcg
     6361 ctcacgaagc gcggcccgta ggcgcggctc gcgtagacgc ggccgaaggc gcgcaggccc
     6421 gccaccggct tgtcgtcgcc ggcgctaagc gggtcggcca gcaacgcggc ctcgaggccg
     6481 cgcaggaaca gcaggttgtg cggatggcag cgcgcggctg aacaccacgc cgcgacgcgc
     6541 cgcacgcgct gcgccagcag gcagccccat tgcaggctct gcattccgcc gagtgaccag
     6601 ccggcgacga gcgccagcgt gacatcgccg aacagcatgt ccaccagccg cgcctgggcc
     6661 atgacctggt cggccagcgt gatctgcgga aaccgtgcgc cggcctgcgc gggatgcgcg
     6721 ttcgagggcg aggtcgactc gcccgcgccc agcatcgccg ggatgagcag gcaccagcgc
     6781 ggatcggcca gcgggctcgc gccgtcgccg agcgcgcggt tgcccgccat tgcaccaccg
     6841 tagtacgtcg gcagcagcac gatgttgtct ttcggtgcat tcgggtagcc acgctgcagg
     6901 tagcggatgc gcgtgtcgcg cagcaccgcg ccgcactgca ggggcatgtc gcccagcggc
     6961 agctcgtgct caatccattg ttccgtctgc attttccttt cctcctggcg gcgggcgtgg
     7021 agcccgtatc gaaatttaca tacacgctgg catgtatatt gaatgaaaca tccgtgccag
     7081 cggcgccgcc gcacgacgca accagcaacg gagataggca caatgtcagc acctcacacc
     7141 gaccacgcgc tcgccacccg gctcgggaag gcgggcgcgc gctacgcgat ggccagctac
     7201 gtggacatcc acgggcgcat caagggcaag atggtcccgc tcgggcacct gggcaacatg
     7261 ctcgcgggct cggagctcta taccggcgcg gcgctcgacg gcgtgccgca ggagatcagc
     7321 gacaacgagg tcgcggcgat gcccgatcct gccagcgcca cgcagtgtcc gtggaacccg
     7381 gacctggtgt ggttcgccag cgacctgtac ctcgacggca agcccttcga ggcctgctcg
     7441 cgcgtcatcc tgaagcgcca gaccgcggcg gctgcggcgc tcggctatac gttcaacctg
     7501 ggtatcgaga ccgagttctt cctgttccgc gacctgcccg gcggcggctt cggccccgtc
     7561 agcgaccgcg acaatctcgg caagccctgc tacgacccgt gcaccctgat ggacaacctg
     7621 ccgatcgtcg acgaactggt gagcgcgatg aacgcgctgg gctgggacgt gtattccttc
     7681 gaccacgagg acgcgatcgg gcagttcgag accgacttca cctatgccga cgcgctcacg
     7741 atggcggacc ggctggtgtt ctttcgcatg atggccaacg agatcgcgcg caagcacggc
     7801 gcgttcgcca gcttcatgcc caagcccttc gcggaccgca ccggcagtgg cgcgcactac
     7861 aacatgtcgc tggcggaccg ggaaaccggc accaacctgt tcgcgcccgc cggcgacgac
     7921 gtgcacggtg tcggggtgtc gaagctcgcc taccacttca cggcgggcgt gctgcgccac
     7981 gcgcgcgcga tcagcgcggt catcgcgccc acagtcaaca gctacaagcg cctggtgcgc
     8041 cagggttcca tgtccggttc gacctgggcg ccggtgttct gctgctacgg caacaacaac
     8101 cgcaccaaca tgatccgcgt gcctgcctgc ggctcgcgca tcgagtgccg ggcggccgac
     8161 atcagctgca acccgtatct gggcgccgcg ctgatcctcg cggcgggcct cgagggtgtg
     8221 cgtgaacagc tcgaccccgg gctgccgcac cgcgagaaca tgtacaacta ttccgacgcc
     8281 gagattgccg cgcgcaacat ccagttcctg ccccagagcc tggaggaggc catcgatgcc
     8341 ttcgaggccg acccgctctc gcgcgaggtc ttcggcccgc gcatgttcga caccttcgtg
     8401 cagttcaagc gcgccgagtg gcgcagttac cacaaccacg tctccgactg ggagatcgag
     8461 cgttacctga agatgttctg agcgcctgca cgcaggccgc gaattccgtc aaccacgaca
     8521 ccggagtcac accatgagat accccgtcct gaaacgcctt gccgccgtgc tgctcgccgg
     8581 cgcggccgtc gtctgcgctg gcggcgcgag cgccgaggaa aagcagaagt tctcgatcgc
     8641 ctggaccatc tacgccggct ggatcccgtg gaagtatgcc gacgagtccg ggatcatgaa
     8701 gaagtgggcc gacaagtacg gcatcgacgt gaggatcgtg caggtcaacg actacgtcga
     8761 atcgatcaac cagtacaccg cgggccagtt cgacggcgtc gtggccacca gcatggacac
     8821 gctgtcgatt ccctcggcca gcggcgtcga taccaccgcg ctgatcgtcg gcgactactc
     8881 caacggcaac gacggcatcg tgtcgaagca agccgattcg atcgccggct tcaagggcgg
     8941 caaggtgcac ctggtcgagc tctccgtctc gcattacctg ctcgcccgcg cgttggcgag
     9001 cgtcgggctg agcgagcgcg acgtgtcgat cgtgaacacc tccgatgccg acatcgtcgc
     9061 ggcctacgcc acgcccgagg tcacgcacgc ggtgacctgg aacccgttgc tcgaccaggt
     9121 cgccgcgacg cccggcaccc gcaagatcta cgattccagc agcattcccg ggcacatcaa
     9181 ggacctgacc gtcgtcaaca gcgcgacgct cgccgacaac cccgcgttcg gcaaggcact
     9241 ggtgggcgcc tggttcgaga tgatggcaat actcgagagt cccgggcccg aggggcagaa
     9301 gatgcgcgcc atgctcggtg aagcctcggg caccgaccgc gcgggctatg agagccagct
     9361 ggcacgactg aaaatgttct ggaagcccgc cgacgcggtg gccttcgtga agagcgcgca
     9421 ggcctacgag gcgatggatt cggtgcgcag gttctccttt gatcacggcc tgctcggcga
     9481 gggggcgtcg gatgccgatt tcgtggggat cagcttcccg gacggcaagc aactgggcaa
     9541 cgcggccaac acgaagctgc gcttcgacac cacttacatg cagatggcgg ccgatggcaa
     9601 gctgtgaggg aacaccatcc gcaagggggc tgcggccccc gcctggacgc ccatgagacg
     9661 tacccgtgaa gaagccgaga tgacccgcag gcgggtcatc gaggccgcgc tcaagctgtt
     9721 cagccgcaac ggctattcgc agaccacgct ggcgatgatc gccgccgagg ccgggtacag
     9781 ccgcggcccg atctactggc acttcagcaa caaggacgag ctgttcgagg ccgtcatcgg
     9841 ccattcgcag gagcccttgc tgcaactggt ggaacgcgcg cggctggagc cggatccgcg
     9901 ccgtgccatc gacgactttg tcgacgaatg gttccgcctg ctcgtcgagg acgactggtt
     9961 ccggcagtcc ttcgagatcc tgctgaacaa gaccgagctc accgcggcga tggtgcgcac
    10021 gatccggcgc gaacgacagc tcacgcgcgc catcatcgcc accctcgggg cgcgcatccg
    10081 ggagctgcgg cccgcgggcg atgtcgatgc cgagtcactg ggactgctct gctacaccta
    10141 cctgatgggg atcacgcaga gctggctgtt tgccccgaag ctgttctccc ttgccgggcg
    10201 gcgcgatttc ttccgcacgc acctgcacgg gatgctgggt ctcagccggt gacttcctgc
    10261 gccgcctgcg caatttcgcg ctgacgccgt ttctcctgtc gcatcatcca gatgcccgac
    10321 agcaccacga tggccgtgac cacgccgacg atgatcgtcg ccagcgcgtt gatcttgggc
    10381 gaaatgccca tgcgtaccga cgagaacacc accatgggca gggtcgtcga actcggtccc
    10441 gacacgaagg ccgagatcac caggtcatcc agcgatatgg tgaatgccag cagccagccc
    10501 gacaccagcg ccggcgcgat gatgggcaac gtgatcagga agaatacccg cagcggcgtg
    10561 gcgccgaggt ccagcgcggc ttcctcgatc gagaggtcca gttcgcgcag tcgcgaggaa
    10621 accactatcg cgacatagga cgtcgtgaaa gtgacatgcg cgatccacag cgttgtcatg
    10681 ccgcgcccgg ccggccagcc gatcatctgc tccatcgcga cgaacagcag cagcagtgac
    10741 aagccggtga tcacttccgg catcaccatc ggcgccgtga tcaggccgcc cagcaggttg
    10801 cgcccgcggt agcggccgaa gcgcgtgagc gcgatcccgg ccgcggtgcc gagcaccagc
    10861 gaggcgctgg ccgacatgaa gccgaggctc aggctcttcc acgccgcgtc gagtatcgcg
    10921 cggtcctgca gcagcgcggc ataccacttg gtggagaagc cgccccacag cgtgaccatg
    10981 cgcgattcat tgaaggaata gatcaccagc agcagtatcg gcacgtacag gaacgcgaag
    11041 ccgaatacca ggaatccgag cagcaggcgc gagcggcgcg gcgacatcag cccgttccct
    11101 cctgcctgtc cagttccttg ttctgcatgt gctggtggaa cagcaggggc ccgatcagca
    11161 cgatcagcat caccgccgct accgccgagg ccaccggcca atcgcggttg ctgttgaact
    11221 cgttccacag cacgcggccg atcatgagcg tgtccggccc gcccagcagc tcgggaatca
    11281 cgaactcgcc cacggccgga atgaacacca gcatcgatcc ggcgatgatg ccgcgctgcg
    11341 acaagggcag cgttatcttc aggaacgcct tccaggcgga acagcccaga tcctgcgcgg
    11401 cctcgatgag gctgtaatcg tgctttgcga ggttggtgta gagcggcagg atcatgaacg
    11461 gcaggtacgc gtagacgatg ccgatgtaca ccgccacgtc ggtttgcagt atctggatcg
    11521 gctgttctac gagcccgagc cacagcaaca ggttgttgag caggccctcg ttcttcagga
    11581 tgccgatcca ggcgtagatg cggatcagga acgaggtcca gctgggcagc accacgagca
    11641 tcaggaacac gttgcgcagc gactcgctgg agttggcgat cgcgtaagcc atcggatagc
    11701 cgaccagcag gcacagcagc gtggagagtg ccgcgatttt cagcgaactc aggtacgcga
    11761 tgtaatagag ctcgtcttcc cacaggtagc ggtaattgtc gaaattcagg gtgatgtgcg
    11821 cgtggccatc cgcgtcgtac tggatcaggc tggtgaatgg cggaattgcg ttcgcgatct
    11881 cggcgaagct tatcttgagg acgaatccga acggcaccag gaagaacagg atcagccaca
    11941 cgtaggggac catgatgatc ccgaagcgcc cgaagccgaa gcggcgcgct ttcatgcggt
    12001 gagcagcacg ctgtcggcgc cgtcccagct cacgaagacc tcgtcgcccc aggtcggcgg
    12061 ttcctcctcg tgcctgcgcc gggtgttcgc catcgtgcac agcaccttct tgcccgaggg
    12121 cagctgcacg tggtacacgg acatgtcgcc gaagtaggcg atgtcgcgca ccacaccgct
    12181 cgcccagttg tattcctgct ccggttgcac gcgttcgatc tggatcttct ccggacggat
    12241 ggccagccac acgcgcatgc cgtccgggcc gctgatgccg tggtcgacat ggatgtgcac
    12301 gggcaaatcg ttgcatccca ccgtcacgtg gtcgctctcg ttctcgagca ggtcgccctc
    12361 gaagctgttg accaggccga tgaactcggc ggaaaaacgg caattcgggt actcgtatat
    12421 ctcgcgcggc gcgccgacct gcacgaagcg gccctcgctc atgatcgcca cgcgcgaggc
    12481 catcgtcatg gcctcttcct ggtcgtgcgt caccatcacg caggtgacgc cgaccgtctc
    12541 gatgatgtcc accagttcca gttgcatgcg ctcgcgcaat ttcttgtcga gcgcgcccat
    12601 cggttcgtcg agcagcagca gcttcgggcg cttggccagg gagcgcgcca gcgccacgcg
    12661 ctggcgctgc ccgcccgaga gctggtgcgg cttgcgcctg gcataggtct tcatgtccac
    12721 gagttccagc aactccccga cgcgcttgtc cacggtgtcc gcgggcaagc gatcctgctt
    12781 caggccgaag gcgatgttgt cgcgcaccga catgtgcgga aacagcgcgt aggactggaa
    12841 catcatgttg gtcgggcgga tgtagggcgg ggtgccggcc atgtccgcgc cgtcgatgag
    12901 gatgcgtccc gcactcggcg tctcgaagcc ggcgagcatg cgcagcagcg tcgatttgcc
    12961 gcagccggaa gggccgagca gcgcaaacag ctcgcccttg gggatgctca ggctgacatc
    13021 gttgacggcg agcacgccgt cgaaggactt gctcacgttc tcgatgcgga cgtaaccgtc
    13081 ctgcgcggga gctcgcgtgg agctcgctgc ctgctgcaac atcgttcagt gctccactgt
    13141 aagggacccg aaccgccctt ccgctgtcgc cggcgcgcgc tcagcgcccg gtcttgaatt
    13201 tggtccaggc gcgcgtcatc acgcgttcga ccttcggcgg cttgggcgag agaccgaaca
    13261 tccggtcgat ctgttcctgc ggcggataga ccagcgggtc gccggaaagc gacttgtcca
    13321 ccagtgccgt ggccgccttg ttggggttcg gataagtcac gaaatccgtg accgccgcga
    13381 tgacctccgg acgcagcatg tagtcgatga atacatgcgc cgattcgggg ttcggtgcat
    13441 ccgccggtat cgcgagggag tcgaaccaga tctcggtgcc ttccttcggg atgatgtagc
    13501 ggatattgac gccattgccc gcttcctcgg cgcgggcctt ggcgatgccg atgtcacccg
    13561 aaaatcccat cgccacgcag atctcgccgt ccgcgaggct gttgatgtac tccgaggacg
    13621 agaacttgcg gatgtggggg cggatcttca tcagcagctc gttggccgcc ccggtgtagt
    13681 ccgcctcgtc gagggaattc gggttcttgc cgagatggaa cagcgccagc gggtagacct
    13741 cggagggcga atcgaggaac gacacgccgc aggaggccag cttggccatg tactccggat
    13801 tgaacagcag gtcccagctg tccagcgcga cgtcttcgcc cagcaccgcc ctgaccttgt
    13861 cgacattgat gccgatgccc acggttcccc acaggtaggg caccgcgtac tggttgcccg
    13921 gatccacctc ggccacgtac ttcagcatca tcggatcgag gttcttgaaa ttcggcagct
    13981 tgctgcggtc gagtttctgg tgcgttcccg ccgggatctg cttgccgaga aaattcgtgg
    14041 acggcacgac gatgtcatac ccggtgctgc cggcggagag cttgccctgc aggacttcgt
    14101 tgctgtcgta gacgtcgtag gtgaccttga tgccggtctc cttctcgaaa ttcctgatgg
    14161 tgtcctcggc gatgtagtcg ctccagttgt agatgtggag ttccttgcgg tccgcggcgg
    14221 cgacggggcc gcagaccagc aatgccgcgg cggcagccgc aagtccgttc cagaacttca
    14281 tcgaatgtcc tcccgcgttg gtgatcggtt tcctgcacat cgcgaaattc ccctgtcgtc
    14341 gggttgcacc ggattctgcg ctgccggcca ggagtcatca aaggcgccgc atcggagtgc
    14401 gcgatcggtc gcgtcaccaa tatatcaact gaagcgaaaa tatacagcgc gaatgtgcgc
    14461 gtgatgacac cgctcattga acgcgcctgc gccatcccct cgtcgctgaa gacggggcgc
    14521 gggttcgccg ccatcctgtc gaggaaagcg cgaccatcgg gccgcacagg tgcatcggtc
    14581 acggaggcag gctccttgtg gcgatcc
//
LOCUS       DAORUQ010000073        14521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_3994_length_14521_cov_6.453408, whole genome shotgun sequence.
ACCESSION   DAORUQ010000073 DAORUQ010000000
VERSION     DAORUQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3994_length_14521_cov_6.453408"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..764
                     /locus_tag="PLW13_08160"
     CDS             222..764
                     /locus_tag="PLW13_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70386.1"
                     /translation="MSSISPPGNKLWWKEPIEKSELMWIAVVVVWGIILTLMMPFWHV
                     YGKQNLSSEAYRTSPEIYMKKVQAVVDQYTVRTEGDRNTPVVKPPVGTDIYMVGRLWD
                     WWPIFELQVGQSYRLHLASVDWQHGFSLQPANLNVQVLPGYDMVLTVTPNKTGTYSIV
                     CNEYCGIGHHMMLGKIYVVE"
     gene            773..2479
                     /locus_tag="PLW13_08165"
     CDS             773..2479
                     /locus_tag="PLW13_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY70387.1"
                     /translation="MNNQTEFRICPETGLKIHRPAEALIRANAVAAVVFLLIGGVLGI
                     GVGLTRWPEVHLLRADLFYQVLTAHGINVLIFWMIFFEVAVLHVASAILLGSRIATPK
                     LGWAASILMVIGALMNNYAVLVKGNSSVMMTSYVPMPADALFYLGLILFAVGALIHCF
                     IFLGTLVIARGERTYEGSIPLVTFGALTACIIAIFTIASGAVILIPTLLWNLGLVAHI
                     DPGMYRLVWWAFGHSSQQINVAAHVSIWYLTAAVLFGAKPLSEKVSRFAFFLYIAVLQ
                     IASAHHLLVDPGLSSEWKIFNTSYAMYLAVMASMVHGLTVPGSIEAAQRAHGFNSGLF
                     EWLRKAPWGNPVFSAMFLSLVGFGVLGGITGVVMGAEQINLVVHNTVYVPGHFHATVV
                     IGSTLAFMGITYWLVPILFRRDLILKGVARWQPYVFGLGMTGVAMFLMGAGTLGVPRR
                     HWDILFSQAGAGNGYEFSAAALTMMSLNGMSVVLAGLGGAMYVIVVVGSILFGRKLGE
                     NEKLGVEMIKAPPAEEKYHNIGIGSITIPGTLVMLTVFFIAFALYYFINWKFLAQTWG
                     LS"
     gene            2562..3422
                     /gene="cyoE"
                     /locus_tag="PLW13_08170"
     CDS             2562..3422
                     /gene="cyoE"
                     /locus_tag="PLW13_08170"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQY70388.1"
                     /translation="MTHREIIDIFKLRIGALMALTAVAGYVVTPGIRLSSPQLLLLAL
                     VVLGASAAAGAFNQFAEHDLDAKMARTAKRAFVTGAATPDNRWLALIAALLLVSVTLG
                     GWAFNLAVAFHLFMGAFFYGVIYTLWLKRRSPLNIVIGGAAGSFAVLAGAAAADPGLG
                     PESALLAVVLFLWTPPHFWALAIVLHKDYEAAGVPMLPVVKGDAVAARVILVNTVVLV
                     AVSLLPSLFAMGWIYLAGALAGGGYFIFRGMQLVASPTQKVAMRCFLASLVQLSAVLL
                     AAMLDVFLRA"
     gene            3422..4270
                     /locus_tag="PLW13_08175"
     CDS             3422..4270
                     /locus_tag="PLW13_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY70389.1"
                     /translation="MSSQPPAIASFFCALLLALSMTCNATDAAPQGGHGSAAPGDRDA
                     ALRISQAAIGRQVGDYAFYGIDGTRHQLAQYRGKPLVISLIYTSCYHICPTTTRHLAE
                     VIERAQSVLGEDSFAVVSVGFDAARDTPQAMKNFASEQKVAAVHWDFLSSDAQTIDAF
                     ARDLGFQFRPAGGGFDHLLQTTILDADGVVRRQVYGMDYDTPILIEPIKRLIFGEQLS
                     EGFFERMTNKFRLFCTVYDPASDRYRFNYSIFVGIAMGIAMGIFFVFLLVREWRYSRR
                     ARPDSN"
     gene            4290..5864
                     /locus_tag="PLW13_08180"
     CDS             4290..5864
                     /locus_tag="PLW13_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQY70390.1"
                     /translation="MIRKLQQVMLGVLVRTEAAFDSVFGAHWNPWYQLGALSFHFFWI
                     VVVSGVYLYVFFDTSILGAYASVQALTEEQWYLGGVMRSLHRYASDAMVVTVTLHLLR
                     EFAKGRFRGAQTFSWVSGLPLLWLLFAAGIGGYWLVWDQFGQYVAQTTTEWLERLPAI
                     SDSLARTFLSDATLSDRLFSLLVFMHIAIPLFLLVGMFIHVNRLKLARTQPANGLAIG
                     VVVMLVVLSLVKPARSMGPADLKTAVASVDLDWVYMNFYPLLDRMDPLYVWIMLAGIT
                     GLLMLLPWLSPQQAPAPQAAVVDPNNCNGCSWCFQDCPYEAITMIPHEFRKGQRQARV
                     DPDRCTACGICAGSCPSATPFRHVEELVSGIEIPGYPIDRLREEAVAKLATLSGEARV
                     MVFGCDHALPIAQIETAGVAALSLPCIGMLPPSFVDYIARQDNVDGVMVSGCCSGDCF
                     YRKGNHWTEERFASRRMPHLRTSAGHDKVEVCWASVFQGEMLAAELDKFRARLAHEPI
                     AGGTPPPAGEAAHEHH"
     gene            5851..6360
                     /locus_tag="PLW13_08185"
     CDS             5851..6360
                     /locus_tag="PLW13_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70391.1"
                     /translation="MNTTRLRRCAGQGAAYALFAAFIGYFSNSPAYQHLPPDQAMVKL
                     SLRHSGQLLGECQQRDPEELAKLPATMRAPADCPRERSPLELVLEVNGETILDEQIMP
                     RGLHKDGMASMYRRVNVPSGPVMLKVRMKDHPGQTEFPYSVEREMTLRPAQVLVIDFD
                     SQNRRFEFL"
     gene            complement(6369..6548)
                     /locus_tag="PLW13_08190"
     CDS             complement(6369..6548)
                     /locus_tag="PLW13_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70392.1"
                     /translation="MEIVLFTIVAVALYFAADWALRTLERIHGAPLPYRNVIYFVIIL
                     VLTLASFEFLQRLGP"
     gene            6639..7640
                     /locus_tag="PLW13_08195"
     CDS             6639..7640
                     /locus_tag="PLW13_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQY70393.1"
                     /translation="MREHAGRLQLSLAPLVALILVATLSIDFLSAYIRHTEAGLGCRD
                     WPACYGQIGESIASVEQPGIARHALTPDPLAKRAHRLLATGLVVMVLLLLHRSRQRGA
                     LTGFEAQIPLLMAALLLLLAVIGPASYLKTRPAIAACNLLGGMALAALSWRLLLGLRR
                     SARVVSSVRLRRLTRMALGLLVLQLALGAWVSANFAGLACGGLLDCAAPANAGTDSVP
                     AAFWYFRELALDADGRVPTTAGSVAIHVAHHAGACLAAFALLAAGVQALRERVPRQVV
                     LPVLALLMAQLGLGAFMVSASLPLPLVLAHSTVAALLLLALLRLDYTLQPASPERRS"
     gene            7637..8230
                     /locus_tag="PLW13_08200"
     CDS             7637..8230
                     /locus_tag="PLW13_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY70394.1"
                     /translation="MSRARALLIAALVLAAGIVAARFAAPLIAPQPPLPVLDALGGEF
                     SLPSTLGRETALAEFRGRLVLLNFGFASCPDVCPTVLSRMREALRGLGKDADAVQPLF
                     VTIDPERDTLEKLKPYVEFFHPAFVAMSGTPEATRAVTGLFKVYYEREDNPQLGYGFA
                     HSDQIYLLDRQGRVRGTFANTATTSEMVATMRRLLAE"
     gene            8256..9647
                     /locus_tag="PLW13_08205"
     CDS             8256..9647
                     /locus_tag="PLW13_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY70395.1"
                     /translation="MNQPEARDLPGSHVVEALRAPDGAPARTVLAWIWLGVYALLAAG
                     MLALLLALSRTPFIHDMVPGSQWFRVALVAHVDLSVLVWFIAGAGMLWSLHGGSAART
                     ASRVAIAFAVIGTVMISVAPFVGAGRPLMNNYVPVLEHPWFYAGLAAVATGLVIQAAS
                     YLLVFLRRAHSRDEDEVISYALGLAALSALLAVACVGISYRGMPASASGEYYFDLLFW
                     GGGHVLQFTHTILMLVASMMLLRASGSRLRGGPRLQAALFTLTVLPLAAVPWINGFPV
                     SSVEHIVNFTSLMRYGGMACIPLLLLVIASLGRHGPLAPAQLPLRGALICAISLFAGG
                     GIMGFMIRGLNTVIPAHYHGSIVGVTLAYMGVVYLLLPRLGRPLTMLRTAHWQPYVYG
                     VGQVMHITALAWTGGHGVQRKTAGAAQGLHSAQEIIGMGLMGLGGMIAVIGGVMFLVV
                     VLAALRRGGPVPR"
     gene            9796..11472
                     /locus_tag="PLW13_08210"
     CDS             9796..11472
                     /locus_tag="PLW13_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70396.1"
                     /translation="MPLRPSVLAIFASLILLAACGPPASPPDGATQPADALQGRVAAH
                     WFGRQWPKNYLPGFRREHVAEDFRALVQDGFDTVVLLVAWGDFQPVYAPCCVYDERAF
                     ERLRFLLDRADEAGLAVILRLGYGWSFHPQAGDVGERQHRVLNDPAARAAFGSFLERV
                     SAEISEHDNVLMGFMSWEDLWLRQVDELGAGTYAEYLATLAPDPARPPGLPDATRDVR
                     LFHGYWDWLVIEKLLAPAVPLFPALSFEARIDSEPRLEPGPDGAPVVAEWITHDGMMR
                     MPQGHPLTIYWAPFWGALNRGEQLPAARSLELLDALLRQSAEKSGRLLFIDQFNFVDN
                     TPGHEHNAVIRPGEIPAFLRDAVCTMKRHDVLGYAIWTARDYAESPLHNPVFGYGLEG
                     WRLERVAGAPEQALQVLPSGDFQLRLADGDRLSQQVPYRHGRMPRAGDALPDRVCVEA
                     EVSAPGVLEARAGGEPVMLDFGATGRQRRCADIPPRPDEEGLEFALQLRGGALALREA
                     MIFDHVQYGGLYDLEGRPGALREPVRRMNRDFRSGPARCAGGWFAGLRRP"
     gene            11469..12362
                     /locus_tag="PLW13_08215"
     CDS             11469..12362
                     /locus_tag="PLW13_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY70397.1"
                     /translation="MNRHGALPFLIACLGIAAFTGMDAIMKGLSISIGVYNAMLWRML
                     AGTVIGGLVFFALRSSWPTRPVLRLHLLRSAIVTVMAMAFFWGLVRVPLAEGIAITFV
                     APLIALYLARLVLDEDIGRAAIIASLLALAGVLTIVGGKLRFDHAAQELPGLASLLFS
                     AVLYAWNLILQRRQAQLASPIEIAFFQNLFVLALLALPAPFLAVVPSPLHLPQIIGSA
                     ALALVALGLLAWAYARAEAQVLLTVEYTAFIWAAALGWVLFGETLSGATLLGTALIVA
                     GCILANYRRRGPPAHVEATAL"
     gene            complement(12448..13104)
                     /locus_tag="PLW13_08220"
     CDS             complement(12448..13104)
                     /locus_tag="PLW13_08220"
                     /inference="COORDINATES: protein motif:HMM:NF033207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="midcut-by-XrtH protein"
                     /protein_id="HQY70398.1"
                     /translation="MTRTGSHAIAGTVALTVLMSPLESLAATVPGSLSYAAAQVVAPA
                     TAVPGLGGGGIVLLGLGLMLAAWLIRGKGIHLAVVLVVAGGLLVLGGGNRGIAQIIAT
                     SLSDPQGGTVQVLDGPQQFNNDSGSPIRITELLAPSCNDGPLDIPGIQPQDLCAAGLL
                     LAADEHCSTDFPGCAPQQCVPDCSGKECGDDGCGGSCGSCGQDQNCQTGICVNVVPLG
                     "
     gene            complement(13157..13861)
                     /locus_tag="PLW13_08225"
     CDS             complement(13157..13861)
                     /locus_tag="PLW13_08225"
                     /inference="COORDINATES: protein motif:HMM:NF037752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70399.1"
                     /translation="MHRWIIAVIALAPLQALAATYVFTGNNYVDPVGVYTTEMRMTGW
                     FTTAEPLPPDMPKTEIGPKGANLVTALSFTDGVFTWTHENSVAAIDSDSLFSVSTDAD
                     GNIDGFHLALMSPLPPHALDSPINVFNIRSDTLVYTRIYNLGTCGGTSPNELCSIIRF
                     PDDPPPGGYVDYLVLPISERPPTGSFVTQLSPGNGAEGGSNPIPTLSVPGLALLGTVM
                     ALLGWRRHRTHASARR"
     gene            14223..>14521
                     /locus_tag="PLW13_08230"
     CDS             14223..>14521
                     /locus_tag="PLW13_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70400.1"
                     /translation="MGKRLDAVIIGGGHNGLVCAFYLARAGLSVRILERRDVIGGAAV
                     TEEFHPGYRNSVASYTVSLLSPRVIADMRLVDHGYRVIERPISNFLPQPDGGYLKL"
BASE COUNT         2149 a         4840 c         4889 g         2643 t
ORIGIN      
        1 ccgagagacg ttccgttcat gcagaaagtc ctcgacaacc cgttcctgct gctgttcatc
       61 ggcatcacca ttccgacgct gacctacatc gtgtggggaa tcatggaact ggtggcgctg
      121 ccgatttcct cgccctagcg gcgggaagca cctgacgcca gggcaaggca tcggaggcgt
      181 gaggcatcac gccatgagaa attcccgagg agaaacagcc aatgagcagc atctcacctc
      241 caggcaacaa gctgtggtgg aaggaaccga tagaaaaaag cgagctgatg tggattgccg
      301 tcgtcgtggt gtggggcatc atcctcacgc tgatgatgcc gttctggcac gtctacggta
      361 agcagaacct ctcgagcgag gcctaccgca cgtcgcccga gatctacatg aagaaggtcc
      421 aggcggtcgt ggaccagtac acggttcgca ccgagggcga tcgcaacacg ccggtggtca
      481 agccgccggt aggtaccgat atctacatgg tcggacggct gtgggattgg tggccgatct
      541 tcgagctgca ggtcggacag agctatcgct tgcacctggc atcggtggat tggcagcacg
      601 gtttctcgct gcagccggcc aacctgaacg tgcaggtgct gcccggctac gacatggtgc
      661 tgaccgtgac gcccaacaag accggcacgt actcgatcgt gtgcaacgaa tactgcggca
      721 tcgggcacca catgatgctc ggcaagatct acgtggtgga gtgaggggcg ccatgaacaa
      781 ccagacagaa tttcgcatct gcccggaaac gggactcaag atccaccgcc ccgccgaagc
      841 cctgatccgg gccaatgccg tcgcggccgt cgtgttcctg ctgatcgggg gcgtgctcgg
      901 catcggcgtc ggcctgacgc gttggccgga ggtccacctg ctgcgggccg acctgttcta
      961 ccaggtgctg accgcgcacg gcatcaacgt gctgatcttc tggatgatct tcttcgaggt
     1021 ggccgtgctg cacgtggcct cggcgatact gctgggatcg cgcatcgcca cgcccaagct
     1081 cggctgggcg gcgtccatcc tgatggtcat cggcgcgctg atgaacaact acgcggtcct
     1141 ggtgaaaggc aactcgagcg tcatgatgac ctcgtacgtg ccgatgccgg ccgacgcgct
     1201 gttctatctg ggcctgatcc tgttcgcagt cggcgcactg atccactgct tcatcttcct
     1261 cggcacgctg gtcatcgcgc gcggcgagcg gacctacgag ggatcgattc cgctggtgac
     1321 cttcggggcg ctgacggcgt gcatcatcgc gatcttcacc atcgcctccg gcgccgtcat
     1381 cctgatcccg acgctgctgt ggaacctcgg gctggtcgcg cacatcgacc ccggcatgta
     1441 ccgcctggtg tggtgggcct tcggccactc ctcgcagcag atcaacgtcg cggcccacgt
     1501 ctcgatatgg tatctgaccg cggcggtgct gttcggcgcc aagccactgt cggagaaagt
     1561 gagccgcttc gcgttcttcc tgtatatcgc ggtgctgcag atcgccagcg cccaccacct
     1621 gctggtggat ccgggcctga gctcggaatg gaagatcttc aacaccagct atgcgatgta
     1681 cctggcggtc atggcgagca tggtgcacgg cctgaccgtt ccgggctcga tcgaggccgc
     1741 gcagcgcgcg cacggcttca actcggggct gttcgagtgg ttgcgcaagg ccccgtgggg
     1801 caacccggta ttctcggcca tgttcctgtc gctggtgggc ttcggtgttc tcggcggcat
     1861 caccggcgtg gtgatgggcg ccgagcagat caacctggtg gtccacaaca cggtgtacgt
     1921 gccggggcac ttccacgcca cggtcgtgat tggctccacc ctggcgttca tgggcatcac
     1981 ctactggctg gtaccgatcc tgttccgccg cgacctgatc ctgaagggcg tggccaggtg
     2041 gcagccctac gtgttcggcc tgggcatgac cggggtggcc atgttcctga tgggcgccgg
     2101 cacgctcggc gtaccgcgcc gtcactggga catcctcttc agccaggctg gcgccggcaa
     2161 cggttacgag ttctcggccg ccgcgctgac catgatgagc ctgaacggca tgtcggtggt
     2221 gctcgcggga ctcggaggcg cgatgtacgt catcgtcgtg gtcggctcga tcctgttcgg
     2281 gcgcaagctc ggcgagaacg agaaactcgg cgtggagatg atcaaggcgc cgcccgcgga
     2341 ggagaaatac cacaacatcg gcatcggttc gatcacgatc cccggcacgc tggtgatgtt
     2401 gaccgtgttc ttcatcgcct tcgcgctgta ctacttcatc aactggaagt tcctggccca
     2461 gacctggggt ctgagctgat gcgctgtcgc cgtccggggg agcggttggg ctccccggac
     2521 ggcgatccca cgacaagatt cctgacgacg agccgccctg catgactcac cgcgagatca
     2581 tcgatatctt caagctgcgc atcggtgccc tgatggcgct gacggcggtc gcgggttacg
     2641 tggtcacgcc cggcatccgg ctgtcgtctc cccaattgct gctcctcgcc ctggtggtgc
     2701 tgggagcttc cgccgcggcc ggcgccttca accagttcgc cgagcacgat ctcgatgcga
     2761 agatggcccg caccgccaag cgcgccttcg tcactggcgc cgcgaccccg gacaatcgct
     2821 ggctggcatt gatagccgcg ctgttgctgg tgtcggtgac gctcggcggc tgggcattca
     2881 acctcgcggt ggcattccac ctgttcatgg gtgccttctt ctacggcgtg atctacacgc
     2941 tgtggctgaa gcgccgctcg ccgctgaata tcgtcatcgg cggcgcggcc ggcagtttcg
     3001 ccgtgctcgc cggcgccgca gcggcggatc cgggtctcgg cccggaatcg gccctgctgg
     3061 ccgtcgtcct gttcctgtgg acgccaccgc atttctgggc gctggccatc gtgctgcaca
     3121 aggactacga agcggccggg gtgccgatgc tgccggtagt gaaaggcgac gcggtcgccg
     3181 cgcgcgtgat cctcgtcaac acggtcgtac tcgtcgcggt ttcgctgttg ccgtccctgt
     3241 tcgccatggg ctggatctac ctcgccggcg cgctcgccgg tggcggttat ttcatcttcc
     3301 gcggcatgca gctggtagcc tcgcccacgc agaaggtggc catgcgctgt ttcctggctt
     3361 ccctggtcca gctctcggca gtgctgctcg ccgcgatgct cgacgtgttc ctgcgcgcgt
     3421 gatgtcctcg cagccccccg ccattgcatc gtttttttgc gcgctgctgc tcgccctgtc
     3481 gatgacgtgt aacgcgactg acgcagcgcc gcaaggcggt cacggctccg cggctcccgg
     3541 cgaccgcgac gcggcactgc ggatcagcca ggccgcgatc gggcggcagg tgggtgatta
     3601 cgcgttctac ggtatcgacg gcacgcgtca ccagctcgcg cagtaccgtg gcaagccgct
     3661 ggtcataagc ctgatctata ccagctgcta ccacatctgt cccaccacca cgcggcatct
     3721 ggccgaggtc atcgagcgtg cgcaatcggt gctcggcgaa gacagctttg ccgtcgtcag
     3781 cgtgggcttc gacgccgcgc gcgatacccc gcaagccatg aagaacttcg ccagcgagca
     3841 gaaggtggcc gcggtgcact gggacttcct gtcctccgac gcgcagacga tcgacgcgtt
     3901 cgcgcgcgat ctgggattcc agtttcgccc cgcgggcggc ggcttcgacc atctgctcca
     3961 gaccaccatc ctcgatgcgg atggtgtcgt gcgccgccag gtctacggca tggactacga
     4021 cacgccgatc ctgatcgagc cgatcaagcg gttgatattc ggcgagcagc tgtctgaagg
     4081 cttcttcgag cgaatgacga acaagttccg gctgttctgc accgtttacg atccggcatc
     4141 cgaccgctac cggttcaact actccatctt cgtgggcatc gcgatgggca tcgcgatggg
     4201 aatctttttc gtgttcctgc tggtgcggga gtggcgctac agccggcgcg cccggcccga
     4261 ctcgaactga acgacaacgc atcgacgaca tgatccgcaa gttgcagcaa gtgatgctcg
     4321 gcgtcctcgt ccgtaccgag gccgcattcg attccgtgtt cggcgcgcac tggaatccct
     4381 ggtaccagct gggcgcgctg tcgttccact tcttctggat cgtggtcgtc agcggcgtct
     4441 acctgtacgt gttcttcgac accagcatcc tcggtgccta tgcctcggtg caagcgctga
     4501 ccgaggaaca gtggtacctg ggcggcgtga tgcgcagcct ccaccgctac gcctcggacg
     4561 cgatggtcgt cacggtgacg ctgcacctgc tgcgtgaatt tgccaagggc cgcttccgcg
     4621 gcgcgcagac cttttcgtgg gtgagcggcc tgcccctgct gtggctgctg ttcgccgcgg
     4681 ggatcggcgg ctactggctg gtgtgggacc agttcgggca gtacgtcgcg cagaccacca
     4741 cggaatggct cgaacgcctt cccgcgatca gcgattcgct cgcgcgaacc ttcctgtccg
     4801 acgcaacgct gagcgaccgg ttgttctcgc tgctggtctt catgcacatc gcgatcccgc
     4861 tgttcctgct ggtcggcatg ttcatccacg tgaaccgcct caagctcgct cgcacccagc
     4921 cggccaatgg cctcgcgatc ggcgtcgtgg tgatgctggt ggtgctgtcg ctggtcaagc
     4981 cggcacgcag catggggccg gcagacctga aaaccgccgt ggcatcggtc gatctcgact
     5041 gggtgtacat gaacttctac cccctgctcg accggatgga cccgctatac gtgtggatca
     5101 tgctcgccgg catcaccggg ctgctgatgc tgctcccgtg gctgtcgccg cagcaagcgc
     5161 ccgcgcccca ggcggcggtc gtggacccga acaactgcaa cggctgcagc tggtgcttcc
     5221 aggattgccc ctacgaagcg atcacgatga tcccgcacga gttcaggaag ggacagcgcc
     5281 aggcgcgggt cgacccggac cgatgcacgg cctgcggcat ctgcgccgga tcctgcccct
     5341 cggccacgcc gttccgccat gtcgaagagc tggtatccgg catcgagatc ccgggttatc
     5401 cgatcgatcg cctgcgcgag gaagccgtgg caaagctcgc gacgctgagc ggcgaagcgc
     5461 gcgtgatggt cttcggttgc gatcacgcgc tgccgatagc tcagatcgag accgcgggtg
     5521 tcgcggcact gagcctgccc tgcatcggca tgctgccgcc ctccttcgtg gactacatcg
     5581 cgcgccagga caatgtcgac ggggtaatgg tcagcggctg ctgtagcggg gactgcttct
     5641 accgcaaggg caaccactgg accgaggagc gcttcgccag ccggcgcatg ccgcacctgc
     5701 gcacatcggc gggccacgac aaggtcgagg tgtgctgggc cagcgtgttc cagggcgaga
     5761 tgctcgccgc ggaactcgac aaattccgcg cccggctggc gcacgagccg atcgccggcg
     5821 gcacgccccc acccgccgga gaggctgcgc atgaacacca ctagactgcg tcgctgtgcg
     5881 ggccagggcg cggcttatgc gctgttcgcc gcattcatcg gctacttctc gaattcgccc
     5941 gcctaccagc atcttccgcc ggaccaggcc atggtcaagc tgagcctcag gcactccggg
     6001 caactgctcg gggaatgcca gcagcgagat ccggaggagc tggcgaagct gccggcaacg
     6061 atgcgtgcac cggcggactg cccgcgcgag cgttcaccgc tggaactggt gctcgaggtc
     6121 aacggcgaga cgatactgga cgagcagatc atgccgcggg gcctgcacaa ggacggcatg
     6181 gcgtcgatgt accgccgcgt gaatgttccg tccggcccgg tgatgctgaa ggtgaggatg
     6241 aaggatcacc cggggcaaac ggagttcccg tacagcgtgg agcgcgagat gacgctgcgc
     6301 ccggcgcagg tgctggtcat cgacttcgat tcccagaaca ggcgcttcga gttcctgtag
     6361 gtgaccaact acgggccgag gcgctgcagg aactcgaagc tcgccagcgt gagcaccagg
     6421 atgatcacga agtagatcac gttgcgatac ggcagtggcg cgccgtggat gcgctccagg
     6481 gttctcaagg cccagtcggc ggcgaaatag agcgccaccg cgacgatggt gaagagcaca
     6541 atctccaatg tcgcgtctcc tcgcgattga aggggagcga tgcatgaccg agaatcacac
     6601 gccgatgacg ctcaccccgg gagagcacag gttaaacgat gcgagaacat gccggaagat
     6661 tgcagctgtc actcgcgcct ctcgtggcgc tgatcctcgt tgcgacgctg tcgattgatt
     6721 tcctgagcgc ctacatccgc cataccgaag ccgggctcgg ctgccgtgac tggccggcct
     6781 gctacgggca gatcggcgag tccatcgcca gcgtcgagca gcccggcatc gcccgccacg
     6841 cactcacgcc cgacccgctc gccaagcgcg cccaccgcct gctcgccacg ggactggtcg
     6901 tcatggtgct gttgctgctg catcgcagcc gccagcgcgg cgcgctcacg ggcttcgagg
     6961 cccagatccc gctgctgatg gcggcgctgt tgctgttgct ggccgtcatc gggcctgcga
     7021 gctacctgaa gacgcgcccg gcgatcgccg cctgcaacct gctcggcggc atggcgctcg
     7081 cggcgttgag ctggcgcttg ctgctggggc tgcggcgcag cgcgcgggtc gtatccagcg
     7141 ttcggctgcg acgcctgacg cgcatggcgc tgggcctgct cgtgctgcaa ctggcgctcg
     7201 gtgcgtgggt cagcgcgaat ttcgcgggcc tggcgtgcgg cggcctgctc gactgcgcgg
     7261 cgccggcgaa cgccggcacg gactcggttc ccgcggcctt ctggtatttc cgcgaactgg
     7321 cgctcgacgc agacggtcgt gtgcccacga cggcgggatc ggtggcaatc cacgtggcgc
     7381 atcacgccgg tgcctgcctc gcggcattcg cgttgctcgc ggcgggagtg caggcgctgc
     7441 gcgagcgcgt gccccgtcag gtcgtgctgc cggtgctcgc gttgctgatg gcgcagctcg
     7501 ggctcggcgc gttcatggtg agcgcctcgt tgccgctccc gctcgtgctc gcccacagca
     7561 cggtcgccgc gctgctgctg ctcgcactgc tgcgtctcga ttacacgctt cagccggcgt
     7621 cgccggagcg ccgctcatga gccgcgccag agctttgctg atcgccgccc tcgtgctcgc
     7681 ggctggcatc gtcgcagccc gcttcgccgc gcccctgatc gcgccccaac cgccgctgcc
     7741 ggtgctcgat gcgctgggcg gcgagttctc gctgcccagc acgctggggc gcgaaaccgc
     7801 gctggcggag ttccgcggca ggctggtact gctcaacttc ggcttcgcga gctgccccga
     7861 tgtgtgtccg accgtgctct cgcgcatgcg cgaggcgctg cgcgggctcg gcaaggacgc
     7921 cgatgcggtg cagcctctgt tcgtcacgat cgaccccgag cgcgacacgc tcgagaagct
     7981 gaaaccctat gtcgagttct tccatccggc cttcgtcgcg atgagcggca cgcccgaggc
     8041 aacgcgcgcg gtcacggggc tgttcaaggt ctactacgag cgggaggaca atccccagct
     8101 cggttacggt ttcgcccaca gcgaccagat ctacctgctc gaccgccagg gccgcgtgcg
     8161 cggcactttc gccaataccg ccacgaccag cgaaatggtc gctaccatgc gccggttgct
     8221 cgccgaatga cgatgccggt cacaggaaac agcgcatgaa ccagcccgaa gcccgcgacc
     8281 tgccgggctc gcacgtcgtg gaagccttgc gcgcgcccga cggtgcgccg gcgcgcaccg
     8341 tgctcgcgtg gatctggctc ggcgtctacg cgctgctcgc cgccggcatg ctggcgctgt
     8401 tgctggcgct gtcgcgcaca ccgttcatcc acgacatggt gcccggatcg cagtggttcc
     8461 gggtcgcgct ggtggcgcac gtggatctct cggtactggt gtggttcatc gcgggcgccg
     8521 gcatgctgtg gagcctgcac ggtggctcgg ccgcgcgcac ggccagccgc gtcgcgatcg
     8581 cgttcgccgt gatcggtacc gtgatgatca gtgtcgcgcc tttcgtcggc gccgggcgtc
     8641 cgctgatgaa caattacgtg ccggtgctgg agcacccgtg gttctacgcg gggctcgcgg
     8701 cggtcgcgac cggcctggtg atacaggccg caagctacct gctggtattc ctgcgccgcg
     8761 cgcactcgcg cgacgaggac gaagtgatca gctacgcact cggactcgcg gcactgagcg
     8821 cgctgctggc ggtcgcctgc gtgggtatca gctaccgtgg catgcccgcg agcgcctccg
     8881 gcgaatacta cttcgacctg ctgttctggg gcggcggcca cgtgttgcag ttcacgcaca
     8941 cgatactgat gctggtcgcc tcgatgatgc tgctgcgtgc ctcgggctcg cgcctgcgag
     9001 gcgggccgcg gttgcaagcc gcgctgttca cgctgaccgt gctgccgctc gcggcggtgc
     9061 cctggatcaa cggcttcccg gtgtcctcgg tggagcacat cgtgaatttc acgtcgctga
     9121 tgcgctacgg cggcatggcc tgcattccgc tcctgctgct ggtgatcgcg agcctgggac
     9181 ggcacggccc cctcgcgccc gcccagttgc cgctgcgcgg cgcgttgatc tgcgcgataa
     9241 gcctgttcgc gggcggcggc atcatgggct tcatgatccg cggcttgaat accgtgatcc
     9301 ccgcgcacta ccacggctcg atcgtcggcg tgacgctggc gtacatgggc gtggtgtacc
     9361 tgctgctgcc tcgcctcgga cgccccctga ccatgctgcg cacggcgcac tggcaaccct
     9421 acgtctatgg cgtcgggcag gtcatgcaca tcaccgcgct ggcgtggacc ggcgggcacg
     9481 gggtgcaacg caagaccgcg ggagccgcac agggcctgca cagcgcgcag gagatcatcg
     9541 gcatggggct gatggggctc ggcgggatga tcgcggtgat cggcggggtc atgttcctgg
     9601 tggtggtgct cgcggcactg cggcgcggcg gcccggtgcc gcgctagccg atctgctgca
     9661 gcgcggcgtc acaggcgtcg cggcagacaa cggcggggcg tggagagggc ggggcgtgga
     9721 gagggtggtt gcggatcgcc ctgccgctta tgcttgcgcc acgccaatcc cgcactggat
     9781 cccggaccca cccgcatgcc ccttcgaccg agcgtgctcg cgatttttgc gagcctgatc
     9841 ctcctcgccg cttgcggccc tcctgcctcg cccccggacg gcgcgaccca acccgcggac
     9901 gcgctgcagg ggcgcgtcgc cgcgcactgg ttcggtcgcc agtggccgaa gaactacctg
     9961 cccggattcc ggcgcgagca cgtggccgag gacttccggg cgctggtgca ggacggcttc
    10021 gacaccgtgg tgctgctggt ggcctggggc gatttccagc ccgtctacgc accgtgctgc
    10081 gtctacgacg agcgcgcctt cgagcgcctg cgcttcctgc tcgatcgcgc cgacgaggcc
    10141 ggcctcgccg tgatacttcg cctcggctac ggctggagct tccacccgca ggccggcgac
    10201 gtcggcgaac gccagcatcg cgtgctcaac gacccggccg cgcgtgccgc cttcggcagc
    10261 ttcctcgagc gggtgtcggc ggagatcagc gaacacgaca acgtgctcat gggcttcatg
    10321 agctgggagg acctgtggct gcggcaggtg gacgagcttg gcgccggcac ctatgccgag
    10381 tacctggcca cgctggcgcc cgacccggcg cgcccgccgg ggctgccgga cgccacgcgc
    10441 gatgtgcgcc tgtttcacgg ttactgggac tggctggtga tcgagaaact gctcgcgccc
    10501 gcggtgccgc tgttcccggc gttgtcgttc gaggcgcgca tcgacagcga gccgcgcctg
    10561 gaacccggcc ccgacggcgc accggtggtt gccgagtgga tcacccacga cggcatgatg
    10621 cgcatgcccc aggggcatcc gctgaccatc tactgggcgc cgttctgggg cgcgctgaac
    10681 cggggcgaac agctgcccgc ggcgcgcagc ctcgaattgc tggacgcgct gctgcgccag
    10741 tcggccgaaa aatcggggcg gctgctgttc atcgaccagt tcaacttcgt cgacaacacg
    10801 cccggccacg agcacaacgc ggtgatccgt cccggggaga tcccggcatt cctgcgcgac
    10861 gccgtgtgca cgatgaaacg ccacgacgtg ctgggctacg ccatctggac cgcgcgcgac
    10921 tacgccgaga gcccgctgca caaccccgtc ttcggctatg gcctggaggg ttggcggctc
    10981 gagcgcgtgg cgggagcgcc ggagcaggcg ctgcaggtgc tgcccagcgg cgacttccag
    11041 ttgcgcctcg ccgacggcga ccgcctgtcc cagcaggtgc cgtaccggca cgggcgcatg
    11101 ccgcgcgccg gtgacgcact acccgatcgt gtctgcgtcg aggccgaggt ttccgcgccc
    11161 ggcgtgctgg aagcgcgtgc aggtggcgag ccggtgatgc tcgatttcgg ggcgaccggg
    11221 cgccagcgtc gctgcgccga catcccgccg cggcccgacg aggagggact ggagtttgca
    11281 ttgcagttgc gcggcggtgc gctcgcgctg cgcgaggcca tgatcttcga ccacgtgcag
    11341 tacggcgggc tgtacgacct cgagggcagg cctggcgcgc tgcgtgagcc ggtgcggcgg
    11401 atgaaccggg acttccgctc cgggccggcg cgctgcgccg gggggtggtt cgccggtctg
    11461 cgccggccat gaaccggcat ggcgcgctgc cgttcctgat cgcctgtctc ggcattgcgg
    11521 cattcaccgg gatggacgcg atcatgaaag gcctctcgat ctcgatcggc gtctacaacg
    11581 cgatgctgtg gcggatgctt gccggcacgg tcatcggagg cctggtgttt ttcgccctgc
    11641 gctcgtcgtg gccgacgcgg ccggtattgc gcctgcacct gctgcgcagc gccatcgtga
    11701 ccgtcatggc gatggccttc ttctgggggc tggtgcgggt accgctcgcc gagggcatcg
    11761 ccatcacctt tgtggcaccg ctgatcgcac tgtacctggc caggctggtt ctcgacgagg
    11821 acatcgggcg tgccgcgatc atcgcctcgc tgctcgccct ggcgggggtg ctgaccatcg
    11881 tcggtggcaa gctgcgcttc gatcacgccg cgcaggagct gccggggctg gcatcgctgc
    11941 tcttctcggc ggtgctgtac gcctggaacc tgatcctgca gcgccgccag gcgcagcttg
    12001 cctcgccgat cgagatcgcg ttcttccaga acctgttcgt gctggcgttg ctcgcgctgc
    12061 cggcgccgtt cctggcggtg gtgccctcgc cattgcatct gccgcagatc atcggcagtg
    12121 ccgccctggc gctcgtggcg ctcggcctgc tcgcctgggc ctatgcgcgc gccgaggcgc
    12181 aggtgctgct cacggtcgag tacaccgcct tcatctgggc cgcggcactg ggctgggtgc
    12241 tgttcggcga gacgctgagc ggcgcgaccc tgctgggcac cgcgctgatc gtggccggct
    12301 gcattctggc taactaccgc cgacgcgggc cgccggcgca cgtggaggcc acggcactgt
    12361 aggcccacgc tccgaggccg cactggttcg gtccgcagtt cgtcggcgat caggccccgg
    12421 tgccgcggcg cgatgcccgc cgcggtctca gccgagcggc accacgttca cgcagatgcc
    12481 ggtctggcag ttctggtcct gcccgcagct gccgcagctg cctccgcaac cgtcgtcacc
    12541 gcactccttg ccgctgcagt cgggcacgca ttgctgtggc gcgcagccgg gaaaatcggt
    12601 gctgcagtgc tcgtccgcgg ccaggagcag tcccgccgca cagaggtcct ggggctggat
    12661 ccccggaatg tccagcgggc cgtcattgca gctcggcgcc agcagttccg tgatacggat
    12721 ggggctcccg ctgtcattgt tgaactgttg cgggccgtcc agcacctgga ccgtgcctcc
    12781 ttgggggtcg ctcaggctgg tggcgatgat ctgcgcgata ccccggttgc cgccaccgag
    12841 taccagcagg ccgcccgcga cgacgagcac tacggccagg tggatgccct tgcctcggat
    12901 cagccacgcc gcgagcatca gtccaagtcc gagcaggacg ataccccctc cgccgagccc
    12961 gggaaccgcg gtggccgggg cgaccacctg cgcggcggcg tagctgaggc tgccgggcac
    13021 cgtggcagca aggctctcga gcggtgacat caggacagtc agggcgactg ttccagcaat
    13081 ggcatgcgat ccggttcgtg tcatggtatg catcctggca agattcctca tcgactggtg
    13141 aatcagcgtt gcgcggctag cggcgggcac ttgcatgtgt gcggtgcctg cgccagccga
    13201 gcaacgccat tacggtgccg agcagcgcca gacccgggac cgacagtgtg gggatcggat
    13261 tggacccgcc ttccgcgccg ttgcccggcg agagctgcgt cacgaagctg cccgtcggcg
    13321 gtctctcgct aatcggtaaa acgagatagt cgacatagcc gccgggcggc gggtcgtccg
    13381 gaaaacggat gatgctgcag agctcgttgg gcgatgttcc gccgcaggtt ccgaggttgt
    13441 agatgcgcgt gtagaccagc gtgtcgctgc ggatgttgaa cacgttgatc ggcgagtcca
    13501 gggcgtgcgg cggcagcggg ctcatcaagg ccaggtggaa accgtcgatg ttaccgtcgg
    13561 cgtcggtcga tacggagaac aacgagtcgg aatcgatcgc ggcgaccgaa ttctcgtgtg
    13621 tccacgtgaa caccccgtcg gtgaagctga gtgcggtcac gagatttgcc cccttcggcc
    13681 cgatctcggt cttgggcatg tcaggaggca gcggctcggc ggtagtgaac cacccggtca
    13741 ttcgcatctc ggtcgtatac acgccaacgg ggtcgacgta gttgttgccg gtgaagacgt
    13801 aggtggccgc cagcgcctgc agcggcgcga gcgcaatcac cgcgatgatc catctgtgca
    13861 tgtcggcttc cttggcgggg gtgtcggcgc actctacgta aaaaaccttt cgaataaaac
    13921 cttttattgc gtggaccgct atacgtctgc gtcgccattt cgtcctcgcg ccggatcgcc
    13981 ggcaggtgct cgggcgcgac cgcgaactgc accgtgatac cggccgccac gcgcacgcgc
    14041 gccgagtgcc tgtgcgcgag cgagcgtgtc acgagcggcg cttgttggcg tgatgccgtt
    14101 cccatacagt cgttcggcga atccggtcgc gggtcattcc agactgaatc gagccggtgc
    14161 cgattcctgt tcgcgagccg ccggcacgct ggcgggcgtg ccttcggcca agaggggatc
    14221 tcgtgggcaa aagacttgac gcggtgatca tcggcggcgg gcacaacggc ctggtctgcg
    14281 ctttctacct ggcgcgggcc gggctgtcgg tgcgcatcct cgagcgccgc gacgtgatcg
    14341 gcggcgcggc ggtgaccgag gaattccacc ccggctaccg caactcggtg gcgagctaca
    14401 ccgtcagcct gctcagcccg agggtcatcg ccgacatgag gctcgtcgat cacggctacc
    14461 gcgtgatcga gcggccgatc tcgaatttcc tgccccagcc cgacggcggc tacctgaagc
    14521 t
//
LOCUS       DAORUQ010000074        14512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4000_length_14512_cov_6.873625, whole genome shotgun sequence.
ACCESSION   DAORUQ010000074 DAORUQ010000000
VERSION     DAORUQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_14512_cov_6.873625"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLW13_08235"
     CDS             complement(<1..923)
                     /locus_tag="PLW13_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY70401.1"
                     /translation="MSLALKDPGLLRTRAFIDGQWIDADSGETLAVLNPASGETIAEV
                     ARCGQAETRRAIAAAERAQKDWARRTAKERAVLLRKLFDLMMANQEDLARILTAEQGK
                     PLAEARGEIAYSANFVDWFAEEARRVYGDTIPAHAADKRIVVIKQPIGVVACITPWNF
                     PSAMLGRKIAPALAAGCAVVCKPANNTPLSAFAIAVLAERAGFPPGVINVLGGRTREI
                     GDELCSNPAVRKLTFTGSTPVGKELMAKCAATVKRTSMELGGNAPFIVFDDADLDAAV
                     AGAIASKYRNAGQTCVCANRMLVQDGVYEAFT"
     gene            complement(1166..1708)
                     /locus_tag="PLW13_08240"
     CDS             complement(1166..1708)
                     /locus_tag="PLW13_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HQY70402.1"
                     /translation="MNKSMLIGGVLGGAVAIAGGGIAGYKMLEEPKYAEVVAVTPVTE
                     RIETPREECHQEQVTRRKPVKDQHQVVGTVLGAVAGGLLGDALGGGGSNKGAKIAGAA
                     AGGYAGNKTQEKMQQGATYTTTETVCNTVTDVSERTVGFDVDYRIGDKAGRVRMDHDP
                     GDVIPLRDGQLVLASAPAAQ"
     gene            1890..2420
                     /locus_tag="PLW13_08245"
     CDS             1890..2420
                     /locus_tag="PLW13_08245"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HQY70403.1"
                     /translation="MMRISPLLPLLALALGGCASLSESECADADWEQIGYRDGAAGTS
                     GQRIAAHARACAEYGFTVDEETWRAGYDRGIDSYCTPANAVREGLSGAMYAGVCPGET
                     GVMFERYYQAGREVQVQRQRIDSLESRRHTLMDALDDADDEDTRRTLLNELRYLDATM
                     RAEFDRLRYLEYRLGP"
     gene            2528..5677
                     /locus_tag="PLW13_08250"
     CDS             2528..5677
                     /locus_tag="PLW13_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQY70404.1"
                     /translation="MITRGSVLRHIPFVDHRGLLLAPGNARPNAPSADRSKGRVSLWE
                     RVVAPAVRVMVGVLVLGAVLALGFVAQDEIRHATLQSRWLAAFASGISFELGEGPARA
                     PLRPDRGPYDIRLGYAALPELAPRLEKAGFAVERQAVPSRRMSELAARDFFNVYPEKS
                     QAGLRLLDLNGAVVQQARYPRNVYADFAAVPDLVARTLLYVEDRELLDPARPTMNPVV
                     DWERLGVAVGQQALKSLGRQQKVIGASTLATQLEKFRHSPEGITRDARDKLLQMGSAS
                     LRVYSGGQYTLPARQQLLLNYLNALPLAAQPGYGEVASLGDGLESWFGSPFAEVNRVL
                     ADPAAPLAERAHYYKQVLALILAVRRPTHYLRDTAALAALTDSHLRRMARDGVIPGDL
                     AAAAQAAPLVLRARAGASPVVDFTRQKGVNLARTQLLSLLGVRSLYELDRFDLTARTT
                     LDATVQQEVTTFLRGLADPARIGELGLTGTRLLRASDPSKVVYSLTLYERGEGVNHLR
                     INADNLDQPLDINAGAKLDLGSTAKLRTLVSWLELIAEVHERSVAAPDLARARAELHP
                     RDRLSAWVLDYLGANPGASLADTLAAAMQRRYSAATGQTFYTGGGAHRFGNFESRDNN
                     AVMTVAEALRRSVNLVFIRMMREVVDHYMYRAPSTTARLLENAADPGRGEYLKRFADR
                     EGSVYLRRFYQKYHGKSRDEALRTLLDGVRVTPRAVVVALRSVWPGMQAQDLAPWLAR
                     YVPDEQFTAEGREALFQRYPPEKFNLNDRGYLARVHPLELWLIDYLQRHPEATRDEVL
                     AAGTEVRQEVYGWLMKTSRRRAQDRRILDLLEIEAFQQLHRQWQRLGYPFASLTPSLA
                     TAIGSSGDRPAALAELMGTIVQGGVRYPSRLVTGLHFAAGTPYEVEYAAQSAEGKRVM
                     HPEVAQTARAALLDVVQEGTARALLPQLVRPDGSSHLVGGKTGTGDHRFEVYASPGRL
                     LESRVVNRVATFVFMIDDRFFGTITAFVPGAQAAQYEFTSGLPVRLLGALMPTLAPLL
                     DGTPSMLALAANASPATPP"
     gene            5812..6621
                     /locus_tag="PLW13_08255"
     CDS             5812..6621
                     /locus_tag="PLW13_08255"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: protein motif:HMM:NF009092.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HQY70405.1"
                     /translation="MKNVLGYEGRKVVITGAASGMGAAAARLLVELGAEVHALDIGEV
                     TAPVAKAIRTDMKNKASIDAALAQLPGEIYALFNCAGVPHPPFSAFDTMMINFVGLKH
                     LTDALLPRIGKGGAIASIASTAGMGWKANLELVRKFLALEGFDAAEAWLKDAPELNAD
                     AYGFSKQCLIVYTMTMAGELASRDVRINCISPSPTATAFMDQLTQQVPADAIKLFCPS
                     NGRFAEPAEMGEALVLLNSRLAGFVSGLNVPVDYGYCAEVAMGQRDNLMGI"
     gene            complement(6674..7444)
                     /locus_tag="PLW13_08260"
     CDS             complement(6674..7444)
                     /locus_tag="PLW13_08260"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY70406.1"
                     /translation="MHDAENLFDVSGKVVLITGGSRGLGRAMSLAFARRGAKVVIASR
                     KIGSCEQLAAQITAQGGTAMALACHVGQWDALEGVVDAVEARFGRLDVLINNAGMSPT
                     AESLLATGEALFDKIIDINLKGPTRLTALAATRMGRNGGGSIVNISSLASLKPTPLTT
                     VYSAAKAGLNALTRASAQEYAPLGVRVNCIVCGTFDTDATAGFVRNPAFLAEVVKPIA
                     LARVGRADEVVGAALYFASAASSYTTGAIMNIDGGVMP"
     gene            7679..10189
                     /locus_tag="PLW13_08265"
     CDS             7679..10189
                     /locus_tag="PLW13_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70407.1"
                     /translation="MSTQAAGFRFQRSVLALMIAAGGGSAVPALAQDGRASALEEVVV
                     TAQRREESLQDTPISITAFTPDRIADLGIHNVTDIGEFAPNISIAKQPGSNVSLAIYI
                     RGVGSAESSVVNDPKVGVYVDGVYVSKAVGGVFDVVDLERVEVLRGPQGTLFGRNTTG
                     GAVNITTKKPTGEFGLKANGTLGNYDTQRYGLRMDLPEYYNVAANLSVNHFQTDGWAD
                     NRYDGAGISSLQSDIEDTVASEDNWAYRLALRWTPLDSVTVDYAFDRTDNEGVPSPFQ
                     LTGVRTELFNGFTRRPFPFATLGGSLYQQMAAQVGDPDDRKKNLTLDNSTLEWTDIDG
                     HTVNAAWEVNDALTLKYIFGYRYTDNGNTGSDTDGGTYYARDLFYGVLQGNNGEILSP
                     GFSGVTSKNHVDAKTHELQFIGNLFDERLFYTGGLYYFDETTQQDNPQTFSLPIALVA
                     PRSPALPPLYQALGFGCAPPAPAGLLCGSQRLPFPASDPGIPGFVDFRYGQESESWAA
                     YAQFTYEVTESFDFTAGIRYTEDDKDAWLENDNIDTDPVAPGTQTARLQADDQWDNIS
                     YVVSGNYSITDDVSVYLKYSTGYNAGGFSARATTVSGFEAPYDEEEVEVWELGLKSEW
                     LDNRVRLNAAVFTNDYTDMQIAQFEAGAGGASSRQVNAGAATYEGFELEFVVVPVEGL
                     TLDANYGYLDTEFDEYMQLNPATNRLENISDIAVATYAPENTWNAGAQYDFAPFSFGA
                     LSARLDVTYRDSLVFHPFLNDLTAADDYTLVNGRISLNDIDLGSCCGKGAMRVSLWGK
                     NLADEEYRNFGIDFDSIGFAGNRFGWPRTYGLDVEYAL"
     gene            complement(10307..11503)
                     /locus_tag="PLW13_08270"
     CDS             complement(10307..11503)
                     /locus_tag="PLW13_08270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY70408.1"
                     /translation="MTTAQTPLAGIRVLDIGTMLAGPWAAAMLADQGAEVFKIEAPGI
                     GDVMRYIGPTRNGVSALFHSVNRGKRSLALDLKSAEGREVVQRLAAHTDVLIHNFRPG
                     VPERLGVDYATLAAINPALVYVSVSGFGHSGPMADRPAYDNVIQAFTGVALSQAHAET
                     GEPTQYYQIFADKVTAMYAAQAISVALLARERGAGGQELRLAMVDAVASFMWPDVGGM
                     ALFREEGASPGLAVARHVPLIKCRNGYAQAAPLNDAQFHGWCAAFGVDSSDPDVRTVA
                     DRNRHGGKLQALAAAIYANALGMDVDEVVARLEAADVPCAKAHSLDELPAHPQMQANG
                     LFVESEHPVAGRLLEPRSPVRFGATPTGCGFPSAALGQHSEDILREFGFDAHTVAAWR
                     EKGVIG"
     gene            complement(11565..13712)
                     /locus_tag="PLW13_08275"
     CDS             complement(11565..13712)
                     /locus_tag="PLW13_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY70409.1"
                     /translation="MATREALSFCRLCMGHCGVVVTLDDEQRLATVRGDHEDPQTLGY
                     ACFKGMMAPEAHNSPARILHPLKRMPDGSFQPVPLAQALEEIAAKLGATLAAHGPEAI
                     AGYKGGGAFFTSSSLRMLNEFLAAAGSPKAFSSVTIDQSAKAVAAGRIGIWPAGRDPF
                     GRGEVFMIVGANPLVSVSMNGFDTRNPLKRLKQAKARGMKLIVIDPRRTETARFADAF
                     LQPLPGEDCAILAGLIHIILAEGWQDHEFCARYAGDLDVLRAALAPFTPAYVARRADV
                     PEAELRRVAEVFARDSRRGAASSATGPDMSPDSNLAEHLIECLNVICGRFVREGETVP
                     HPGAILPRWPRKAEVIPAPRWWEHGYRSRIGGFGLIDGELPTGILADEILAPGAGQVR
                     ALIVHGGNPASAVPDQQRIVEALRSLELLVTIEPFMTQTARLSHYVLPPTLQYERADL
                     PIFIYENLVSHDPYTRYTPPVARAPAGAGVQDDWVYFWELAQRLGLTLQHFGVALDMR
                     SRPTTDELLAIAARHAPVAFAELQRAARGMMLDAEPQLVEPGDPASTHRFSLAPDDVV
                     EELAQLARAGDEAGRGARHAAYPYRFAVRRLRDAINSACKDLPSVRPRVPTNALYMNP
                     ADLAREGIAEGELVRLSSDAGTIEVVAAADPDLRPGVVSCAHGFGALPGEPADPRTDG
                     ASTNRLISSTRDRETINAMPRMSGFPVQVARIP"
     gene            complement(13860..14512)
                     /locus_tag="PLW13_08280"
                     /pseudo
     CDS             complement(13860..14512)
                     /locus_tag="PLW13_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
BASE COUNT         2246 a         4966 c         4961 g         2339 t
ORIGIN      
        1 gtgaaggcct cgtagacgcc gtcctgcacc agcatgcgat tcgcgcagac gcaggtctgg
       61 cccgcgttgc gatacttcga ggcgatcgcc ccggcgacag cggcatcgag gtcggcgtcg
      121 tcgaagacga tgaaaggcgc gttgccgccg agctccatcg aggtgcgttt caccgtcgcg
      181 gcgcatttgg ccatcagttc cttgccgacc ggcgtgctgc cggtgaaggt gagcttgcgc
      241 accgcggggt tggagcagag ctcgtcgccg atctcgcgcg tgcgcccgcc caggacgttg
      301 atcacgcccg gcgggaagcc ggcgcgctcg gcgagcaccg cgatcgcgaa ggccgacagc
      361 ggcgtgttgt tcgcgggctt gcacaccacc gcgcagccgg ccgcgagcgc cggggcgatc
      421 ttgcgcccga gcatcgcgct cgggaaattc cacggcgtga tgcaggccac cacgccgatc
      481 ggctgcttga tcaccacgat gcgcttgtcg gcggcgtgcg cgggaatggt gtcgccgtag
      541 acgcgcctgg cctcctcggc gaaccagtcg acaaagttcg cgctgtaggc gatctcaccc
      601 cgcgcttccg cgagcggctt gccctgctcg gcggtgagga tgcgcgcgag atcctcctgg
      661 ttggccatca tcaggtcgaa gagcttgcgc agcagcacgg cgcgctcctt cgcggtgcgc
      721 cgcgcccagt ccttctgcgc gcgttcggcc gccgcgatgg cgcggcgcgt ctccgcctgg
      781 ccgcacctgg ccacctcggc gatggtctcg ccgctcgccg ggttcagcac cgcgagcgtc
      841 tcgccggaat cggcatcgat ccactggccg tcgataaacg cccgggtgcg cagcaggccg
      901 ggatccttga gtgcgagcga catggttact cctctgggca aatgggagac agttggtcag
      961 agtctcgata cctcgccgca atcgcccgga gaggaggctg cgaatgatac acggctgcgc
     1021 cctggctgac gcgagggcgg ccgggttgcc gacgttcgac tggagtcgaa cccacgtaaa
     1081 ggtccggcgt cagccggcaa tcttcgtggg tccggattta tccggagaat gtccgcacga
     1141 atgcggaccc acggcggcgg cagcgtcact gggcggcggg cgcgctcgcg agcaccagtt
     1201 gtccgtcgcg cagcgggatc acgtcgcccg ggtcgtggtc catgcgcacg cggccggcct
     1261 tgtcgccgat gcggtagtcg acatcgaagc ccacggtgcg ctcgctgacg tccgtcacgg
     1321 tgttgcagac cgtttcggtg gtggtgtagg tcgcgccctg ctgcatcttt tcctgggtct
     1381 tgttgccggc ataaccgccg gccgcggcgc cggcgatctt cgcgcccttg ttgctgccgc
     1441 cgccgccgag ggcgtcgccg agcagtccgc cggcgaccgc gccgagcacg gtgcccacca
     1501 cctggtgctg gtccttgacc ggcttgcgcc gcgtcacctg ctcctggtga cactcctcgc
     1561 gaggcgtctc gatgcgctcg gtcaccggcg tcacggcgac gacttcggcg tatttcggct
     1621 cctccagcat cttgtatccg gcgatgccgc cgccggcgat cgccaccgcg ccgccgagca
     1681 cgccgccaat gagcatcgac ttgttcatgc aactctcctc ctgtgatcgg tgtcgcggat
     1741 ggtagcagcc acgagccgcg cctgtggtag gtaaaagtgg cgtcaaccgc ctgcgcgctt
     1801 tgtgcgttgc tcgatttttt gcggcccgtc tttcatggcg gggcgggcaa cggtaggctg
     1861 tcagcctggc ttaaaccgcg gtcccggtca tgatgcgaat ttccccgctg cttccgttgc
     1921 tcgcgcttgc gctcggcggc tgcgccagcc tgtccgagag cgaatgcgcc gatgccgact
     1981 gggagcagat cggctaccgc gacggcgccg ccggcaccag cgggcagcgc atcgcggcgc
     2041 acgccagggc ctgcgccgaa tacggcttca ccgtcgacga ggagacctgg cgcgccggct
     2101 acgaccgcgg catcgacagc tactgcacgc ccgccaacgc ggtgcgcgag ggcctctcgg
     2161 gcgcaatgta tgccggcgta tgccccgggg agaccggggt gatgttcgag cgctactacc
     2221 aggccgggcg cgaggtgcag gtgcagcgcc agcgcatcga ttcgctggag agccggcgcc
     2281 acacgctgat ggacgcgctc gatgacgccg atgacgagga tacgcggcgc accctgctga
     2341 acgaactgcg ctatctcgat gccacgatgc gcgccgagtt cgaccgcctg cgctatctcg
     2401 agtaccgcct gggaccctga tcacgttccg tgcaaccgcc gctgcgggaa taagccataa
     2461 agacgggccg gtacgccggg gccggctaga cttcgagcaa atcccttttc ccgcgaaggt
     2521 cttcccggtg atcacgcgcg gcagcgtcct ccgccacatc ccgttcgtgg atcaccgcgg
     2581 gctgctgctt gcccccggca atgcgcgtcc gaacgcgccg tcggcagacc gctccaaggg
     2641 cagggtgtcg ttatgggagc gcgtcgtcgc gccggccgtt cgggtcatgg tcggggtgct
     2701 cgtgctcggc gccgtgctcg cgctcggctt cgtcgcccag gacgagatcc gccacgcgac
     2761 gctgcagtcg cgctggctcg cggcctttgc cagcggcata tcattcgagc tcggggaggg
     2821 gccggcgcgc gcaccgctgc gacccgatcg cggcccctat gatatccgcc tcggctacgc
     2881 cgccttgccg gagctggctc cccggctgga gaaggcgggc ttcgccgtcg agcgccaggc
     2941 cgtgccttcg cgccgcatga gcgagctcgc cgcccgggac ttcttcaacg tctacccgga
     3001 gaagagccag gccggcctca ggctgctcga cctgaacggc gccgtggtgc agcaggcgcg
     3061 ctacccgcgc aacgtctacg ccgatttcgc cgcggttccc gatctggtcg cgcgcacgct
     3121 gctctacgtc gaggaccgcg aactgctcga tcccgcgcgc ccgaccatga atcccgtggt
     3181 cgactgggag cgcctcggcg tggccgtggg gcagcaggcg ctgaagtcgc tcgggcgcca
     3241 gcagaaagtc atcggcgcca gcacgctggc cacgcagctc gagaagttcc gtcattcgcc
     3301 cgagggcatc acgcgcgatg cgcgcgacaa gctgctgcag atgggctcgg ccagtctgcg
     3361 cgtctacagc ggcggccagt acacgttgcc cgcgcgccag cagctgttgc tcaattatct
     3421 gaacgcgctg ccgctggccg cacagccggg ctacggtgag gtggcgagcc tcggcgacgg
     3481 gctggaatcc tggttcggca gccccttcgc cgaggtcaac cgcgtgctcg cggatccggc
     3541 ggcgccgctc gccgagcggg cgcactatta caagcaggtg ctcgcgctga tcctcgcggt
     3601 gcgccgcccg acccactacc tgcgcgacac cgcggcgctg gcggcgctca ccgacagcca
     3661 cctgcggcgc atggcgcgcg atggcgtgat cccgggcgac ctggccgcgg cggcgcaggc
     3721 agcgccgctg gtgctgcgcg cgcgcgccgg tgcctcgccg gtggtcgact tcacgcgcca
     3781 gaagggcgtg aacctcgcgc gcacgcaact gctctcgctg ctcggcgtgc gcagcctcta
     3841 cgaactcgac cggttcgatc tcacggcgcg caccacgctc gacgccaccg tgcagcagga
     3901 ggtcaccacg ttcctgcgcg ggctggccga tccggcgcgc atcggggaac tggggctcac
     3961 cggcacgcgg ctgctgcgcg ccagcgaccc gtccaaggtg gtctacagcc tgacgctcta
     4021 cgagcgcggc gagggcgtga accacctgcg catcaatgcc gacaacctcg accagccgct
     4081 cgacatcaat gccggcgcca agctcgacct cggctccacc gccaagctgc gtaccctggt
     4141 gagctggctg gaactgatcg ccgaagtcca cgagcgcagc gtggctgcgc cggacctggc
     4201 gcgcgcgcgc gccgagctcc acccgcggga ccgcctgagc gcctgggtgc tggattacct
     4261 cggcgccaac cccggcgcca gcctcgccga tacgctggcg gccgcgatgc agcgccgcta
     4321 ttcagcggcc accgggcaga cgttctacac cggcggcggc gcgcaccgct tcggcaactt
     4381 cgagagccgc gacaacaacg ccgtgatgac ggtggccgag gcgctgcgcc gttcggtgaa
     4441 cctggtgttc atccgcatga tgcgcgaggt ggtggatcac tacatgtacc gcgcgccctc
     4501 gaccacggcg cgtctgctgg agaacgccgc cgatcccggg cgcggcgaat acctcaagcg
     4561 cttcgccgac cgcgagggca gcgtgtacct gcgccgcttc taccagaaat accacggcaa
     4621 gagccgcgac gaggcgctgc gcacgctgct cgacggcgtg cgcgtgacgc cgcgcgcggt
     4681 ggtagtggcg ctgcgctcgg tgtggccggg aatgcaggcg caggatctcg cgccctggct
     4741 cgcgcgctac gtgccggacg aacagttcac ggcagagggt cgggaggcgc tgttccagcg
     4801 ctacccgccg gagaagttca acctcaatga ccgcggctac ctcgcgcgcg tgcatccgct
     4861 ggagctgtgg ctgatcgact acctgcagcg gcacccggag gcgacgcgcg acgaggtgct
     4921 ggcggccggc accgaggtgc gccaggaagt ctacggctgg ctgatgaaga ccagccgccg
     4981 gcgcgcgcag gaccggcgca tcctcgacct gctggagatc gaggccttcc agcaactgca
     5041 ccgccagtgg cagcgcctgg gttatccctt cgcctcgctg acgccctcgc tggcgaccgc
     5101 gatcggcagt tccggcgacc gcccggcggc gctcgcggag ctgatgggta ccatcgtgca
     5161 gggcggcgtg cgctatccct cgcgcctcgt gaccgggctg cactttgccg ccggcacgcc
     5221 ctacgaggtc gagtacgcgg cccagtccgc cgagggcaag cgggtgatgc atcccgaggt
     5281 cgcgcagacc gcgcgcgcgg cgctgctcga cgtggtccag gagggtacgg cgcgcgcgct
     5341 gctgccgcag cttgtacgcc ccgacggttc cagccacctg gtcggcggca agaccggcac
     5401 cggcgatcac cgcttcgagg tctatgcctc gccgggacgg ctcctcgagt cgcgcgtggt
     5461 gaaccgcgtg gcgaccttcg tgttcatgat cgacgaccgc ttcttcggca ccatcacggc
     5521 cttcgtgccg ggtgcgcagg ccgcgcagta cgagttcacc agcggcctgc cggtacgcct
     5581 gctcggcgcg ctgatgccga cgctggcgcc gctgctcgac ggcacgccgt cgatgctcgc
     5641 gctggcggcc aacgccagcc ccgcaacgcc cccctgagtg ccccggggcc cgcctgcgca
     5701 ggcgggcgcg attgcatccg gggcattgcc tccggttatc gcggcacagc atgattgcgc
     5761 ccgccgcacg cggcgagtcg tgcaaaggtc cattcggaca aaggagacaa catgaagaac
     5821 gtcctgggtt acgagggcag gaaagtggtg atcaccggtg ccgcctccgg catgggtgca
     5881 gcggcggcgc gcctcctggt ggagctgggc gccgaggtgc atgcgctgga catcggcgag
     5941 gtgacggcgc cggttgcgaa ggcgatccgc accgacatga agaacaaggc gtcgatcgat
     6001 gcggcgctgg cacagttgcc cggcgagatc tacgcgctgt tcaattgcgc cggcgtgccg
     6061 cacccgccgt tttcggcctt cgacaccatg atgatcaact tcgtcggcct gaagcacctg
     6121 accgacgcac tgctgccgcg catcggcaag ggcggcgcca tcgcatcgat agcctccacc
     6181 gcgggcatgg ggtggaaagc caatctcgag ctggtccgga aattcctggc gctcgagggc
     6241 ttcgatgcgg ccgaggcctg gctgaaggat gcgcccgagc tgaacgccga cgcctacggc
     6301 ttttccaagc agtgcctgat cgtctacacg atgacgatgg ccggcgagct ggccagtcgc
     6361 gacgtgcgca tcaactgcat cagcccctcg cccaccgcga cggcattcat ggaccagctc
     6421 acgcagcagg tgcccgcgga cgccatcaag ctgttctgtc cctccaacgg ccgtttcgcc
     6481 gagcccgccg agatgggcga ggcgctggtg ctgctgaaca gccggctggc ggggttcgtg
     6541 agtggcctga acgtgcccgt cgattacggt tactgcgccg aagtggcgat gggccagcgc
     6601 gacaacctga tggggatctg atccccgagg agcgctcgcg gtgggcgccc ggctcgccgg
     6661 caccggacgc cgctcagggc atcacgccgc cgtcgatgtt catgatggcg cccgtggtgt
     6721 agctagaggc cgccgacgcg aagtagagcg ccgcgcccac cacttcgtcg gctctgccga
     6781 cgcgcgcgag tgcgatgggc ttcaccacct cggccaggaa agccgggttg cgcacgaagc
     6841 cggccgtggc gtcggtgtcg aaggtaccgc agacgatgca gttcacgcgc acgcccagcg
     6901 gcgcatattc ctgcgccgag gcgcgggtga gtgcattgag gccggccttg gcggcgctgt
     6961 agacggtcgt gagcggtgtc ggcttcagcg aggcgagcga gctgatgttg acgatcgagc
     7021 cgccgccgtt cctgcccatg cgcgtggctg ccagcgcggt gagccgcgtg gggcctttca
     7081 ggttgatgtc gatgatcttg tcgaacagcg cttcgccggt ggcgagcagc gactcggcgg
     7141 ttggcgacat gccggcgttg ttgatcaata cgtccagcct gccgaaacgg gcctccacgg
     7201 catccaccac gccttccagc gcgtcccact ggcccacgtg gcaggcgagg gccatcgccg
     7261 tgccgccttg cgccgtgatt tgcgcggcca gttgttcgca ggacccgatc ttgcggctgg
     7321 cgatcaccac tttcgcgccg cgccgggcaa aggccagcga catcgcgcgg cccagtccgc
     7381 ggcttccgcc ggtgatcagc accaccttgc cggatacgtc gaacaggttt tccgcatcgt
     7441 gcaagcgctt gtcctcgggg cgaaatcagc ctcgccgttt tagcgcatcg gttcgccgtt
     7501 ggccaccgta atcgcttgcg gctcgcgccc gggcttgcgc gggaccagat tgccgcgtag
     7561 agttaaaacc gtgctgtgca attgttttaa ggcaatcgcg ctgcagcgcg attgccgccg
     7621 aggcggggcg ctggcgcttg caataaccca taagaatgct cacccggagg gtaatgaagt
     7681 gtccacgcaa gcagctggtt tcaggttcca gagatccgtt ctcgcgctca tgatcgccgc
     7741 gggcggcggt tcagccgtcc ctgccctggc ccaggacggc cgcgcgagcg cgctggagga
     7801 ggtggtggtc accgcgcagc ggcgcgagga atcactgcag gacacgccga tctcgatcac
     7861 ggcgttcacg cccgacagga tcgcggatct cgggattcac aacgtcaccg acatcggcga
     7921 gttcgcgccg aacatctcga tcgcaaagca gcccggaagc aacgtcagcc tggcgatcta
     7981 catccgcggc gtcggctcgg ccgagagttc cgtggtcaac gacccgaagg tgggcgtcta
     8041 cgtcgacggc gtctacgtca gcaaggcggt tggcggcgtc ttcgacgtgg tcgacctcga
     8101 gcgcgtcgag gtgctgcgtg gcccgcaggg cacgctgttc gggcgcaaca ccacgggggg
     8161 cgcggtcaac atcacgacaa aaaagcccac cggtgaattc gggctgaagg ccaacggaac
     8221 cctcggcaat tacgacacgc agcgctacgg tctgcgcatg gacctgcccg agtactacaa
     8281 cgtggccgcc aatctctcgg tcaaccactt ccagaccgat ggctgggccg acaaccgcta
     8341 cgatggcgcg ggtatttcgt cgctgcagag cgacatcgag gacactgtcg cctcggagga
     8401 caactgggcc tatcgactgg cactgcgctg gacgccgctg gacagcgtca cggtcgacta
     8461 cgccttcgat cgcaccgaca acgagggcgt gccgtcgccg ttccagctca ccggcgtgcg
     8521 caccgagctg ttcaacggct tcacccgacg cccgttcccg ttcgccaccc tcggcggatc
     8581 cctgtaccag caaatggccg cccaggtggg cgatccggac gatcgcaaga agaatctcac
     8641 gctcgacaac tcgaccctgg agtggaccga tatcgacggc cacacggtga acgcggcatg
     8701 ggaagtcaac gacgcgctca cgctcaagta catcttcggc tatcgctaca ccgacaacgg
     8761 caataccggc tcggacaccg acggcggtac ctactatgcc cgcgatctct tttacggcgt
     8821 cctgcagggc aacaacgggg agatcctgag ccctggcttc tccggcgtca cgtcgaagaa
     8881 ccacgtcgat gcgaagacgc atgaactgca gttcatcggc aacctgttcg acgagcggct
     8941 gttctatacc ggcggccttt actacttcga tgaaaccacg cagcaggaca acccgcagac
     9001 cttctcgctg cccatcgcgc tcgttgcccc gcgctcgccg gcactccccc cgctctacca
     9061 ggcgctgggc tttggctgcg cgccgccggc tccggccgga ctgctgtgcg gcagccagcg
     9121 cctgcctttc ccggccagcg atcccggcat ccccggtttc gtcgacttcc gctacgggca
     9181 ggaatccgag tcatgggcgg cgtatgcgca gttcacctac gaggtcacgg aatccttcga
     9241 cttcaccgcg ggcatccgct acacggagga cgacaaggac gcgtggcttg aaaacgacaa
     9301 catcgatacg gaccccgtcg cccccggcac gcagaccgcc aggctgcagg ccgacgacca
     9361 gtgggacaac atcagctacg tcgtgagcgg caactacagc atcacggacg acgtcagcgt
     9421 ctacctgaag tactcgaccg gctacaacgc gggcggcttc agcgcgcgcg cgaccacggt
     9481 gtcgggtttc gaggccccgt atgacgagga ggaagtggag gtctgggagc tgggcctgaa
     9541 gtcggaatgg ctggacaacc gcgtgcgcct gaatgccgca gtgttcacca acgactacac
     9601 cgacatgcag atcgcgcagt tcgaggccgg cgccggcggc gcctcgagcc gccaggtcaa
     9661 tgcgggcgct gccacctacg agggattcga gctcgagttc gtcgtcgtgc cggtggaggg
     9721 actcacgctg gacgcgaact acggttacct cgacaccgag ttcgacgagt acatgcagct
     9781 caacccggcc acgaaccggc tggagaatat ctccgatatt gccgtggcga cctacgcgcc
     9841 cgagaacacc tggaacgcgg gcgcgcagta tgacttcgct cccttcagtt tcggtgcgct
     9901 gtcggcgcgg ctcgacgtga cctatcgcga cagcctggtc ttccacccgt tcctgaacga
     9961 tctcacggcc gcggatgatt acacgctggt caacggtcgc atcagcctca acgacatcga
    10021 tctgggcagt tgctgcggca agggagcgat gcgcgtctcg ctgtggggca agaacctcgc
    10081 cgacgaggag taccgcaact tcggcatcga tttcgacagc atcggctttg cgggcaatcg
    10141 cttcggctgg ccccgcacct acggcctcga cgtggaatac gcactgtagc gagctgttgc
    10201 acgcgtgatc gatcgggcgg ccgcaaggcc gctctttttc tgggcagcac ccctgtcccg
    10261 ctgaacgcca agcgctgcct gcgtcaacga cgtgccgtgc ccggcgtcag ccgatcactc
    10321 ctttttcgcg ccacgcggcg acggtgtgtg cgtcgaagcc gaactcgcgc aggatgtcct
    10381 cgctgtgctg tcccagcgcg gcgctgggga agccgcatcc ggtgggcgtg gcgccgaatc
    10441 gcacgggact cctgggctcc agcaggcgac cggcaacggg atgctccgac tcgacgaaca
    10501 ggccgttggc ctgcatctgc gggtgtgccg gcaactcgtc caggctgtgt gccttcgcgc
    10561 agggcacatc ggcggcctcc agtcgcgcca cgacctcatc gacgtccatc ccgagcgcgt
    10621 tcgcgtagat ggccgcggca agggcctgga gcttgccgcc gtggcggttg cgatcggcca
    10681 ccgtccggac atccggatcg ctggagtcca cgccgaaagc ggcgcaccag ccatggaact
    10741 gcgcgtcgtt gaggggtgcg gcctgcgcat agccgttgcg gcacttgatg agcgggacgt
    10801 gcctggcgac cgccagcccc gggctggcgc cttcctcgcg aaacagcgcc atgccgccca
    10861 cgtcgggcca catgaagctc gccaccgcat ccaccatggc gaggcgcagc tcctgcccgc
    10921 cggcaccgcg ctcgcgcgcc agcagtgcca cgctgatcgc ctgcgcggca tacatcgcgg
    10981 tgaccttgtc ggcgaagatc tgatagtact gcgtgggctc gccggtctcg gcatgcgcct
    11041 ggctgagcgc cacgccggtg aaggcctgga tcacgttgtc gtaggccgga cggtcggcca
    11101 tgggcccgct gtgcccgaag ccgctgaccg agacgtacac cagtgccgga ttgatcgccg
    11161 cgagcgtggc gtagtccacg ccgaggcgct cgggcacgcc ggggcggaag ttgtggatca
    11221 gcacgtcggt gtgcgcggca aggcgctgca ccacctcgcg gccctcggcg ctcttgaggt
    11281 ccagcgccag cgagcgcttg ccgcgattga cgctgtgaaa cagcgcgctg accccgttgc
    11341 gcgtcggacc gatatagcgc atcacgtcgc cgatgcccgg tgcttcgatc ttgaatacct
    11401 cggcgccctg gtcggcaagc atggccgcgg cccatggccc tgccagcatg gtgccgatgt
    11461 ccaggaccct gatgcccgcg agcggggtct gcgcagttgt catggcggtg ccctcatcgg
    11521 atctgcctgt tgttgtggcg tcgactgtag cgcagcgcaa atgctcaggg aatccgggca
    11581 acctgcaccg ggaagccgct catgcgcggc atcgcgttga tggtctcgcg atcgcgcgtc
    11641 gaggagatga gccggttggt cgaggcgccg tccgtgcgcg gatcggccgg ctcgcccggc
    11701 agcgcaccga agccgtgcgc gcacgagacc acgcccggcc tgaggtccgg gtcggcggcc
    11761 gcaaccacct cgatcgtgcc ggcatcggaa ctcaaacgca ccagctcgcc ctcggcaatg
    11821 ccctcgcgcg ccaggtcggc ggggttcatg tacagggcat tggtgggcac gcgcgggcgc
    11881 accgagggca gatccttgca ggccgaattg atcgcatcgc gcagccggcg caccgcgaag
    11941 cggtacggat aggccgcgtg gcgtgcgccg cggccggcct cgtcgccggc gcgggcgagt
    12001 tgcgcgagct cctccaccac gtcatcgggc gcgaggctga agcggtgcgt cgatgcggga
    12061 tcaccgggct cgacgagctg cggctcggcg tcgagcatca tgccgcgcgc cgcgcgctgc
    12121 agttcggcaa aggcaaccgg tgcgtggcgc gcggcgatag ccagcagctc gtccgtagtg
    12181 ggcctcgagc gcatgtccag cgccacgccg aagtgctgga gcgtcaaccc gagccgttgc
    12241 gccagttccc agaaatagac ccagtcgtcc tgcaccccgg caccggccgg cgctcgcgcg
    12301 acgggcggcg tgtagcgcgt gtacgggtcg tgcgagacca ggttctcgta gatgaagatc
    12361 ggcaggtcag cgcgttcgta ctgcagcgtg ggcggcagca cgtagtgcga gagtcgcgcc
    12421 gtctgcgtca tgaacggctc gatcgtgacc agcagctcca gtgaacgcag cgcctccacg
    12481 atacgctgct ggtcggggac ggccgaggcc gggttgccgc cgtgcacgat cagcgcgcgc
    12541 acctgtccgg cgccgggtgc caggatctcg tcggcgagga tgccggtggg cagttcgccg
    12601 tcgatcagcc cgaagccgcc gatgcggctg cggtaaccgt gttcccacca tcgcggcgcc
    12661 gggatcacct cggccttgcg cggccagcgc ggcaggatcg cgccgggatg cggcacggtc
    12721 tctccctcgc gcacgaagcg gccgcagatc acgttcaggc actcgatcag gtgctcggcg
    12781 aggttgctgt cgggcgacat gtcggggccg gtggcgctcg aggccgcgcc gcgcctgctg
    12841 tcgcgcgcga acacctcggc cacgcgccgc agctcggcct cgggcacgtc ggcgcgtcgt
    12901 gccacgtacg ccggcgtgaa cggcgcgagc gccgcgcgca gtacgtccag atcgcccgcg
    12961 tagcgggcgc agaactcatg gtcctgccag ccctcggcga gaatgatgtg gatgagtccc
    13021 gcgagtatgg cgcagtcctc gcccggcagc ggctgcagga acgcgtcggc gaaacgcgcg
    13081 gtctcggtgc gccgcgggtc gatcacgatc agcttcatgc cgcgcgcctt ggcctgcttc
    13141 aggcgcttca gcggattgcg cgtgtcgaag ccgttcatcg ataccgagac cagcgggttg
    13201 gcgcccacga tcatgaatac ctcgccgcga ccgaaggggt cgcgtcccgc gggccagatg
    13261 ccgatgcgcc cggcagcgac ggccttcgcc gactggtcga tggtcaccga ggagaatgcc
    13321 ttggggctgc ccgcggcggc gaggaactcg ttcagcatgc gcagcgagga cgaggtgaag
    13381 aaggcgccgc cgcccttgta gccggcgatc gcctcggggc cgtgagcggc gagtgtcgcc
    13441 ccgagtttcg ccgcgatctc ctccagtgcc tgcgccagcg gcaccggctg gaacgagccg
    13501 tcgggcatgc gcttgagcgg gtgcaggatg cgcgccggac tgttgtgcgc ctcgggcgcc
    13561 atcatgccct tgaaacaggc gtatccgagc gtttgtggat cctcgtgatc gccgcgcacg
    13621 gtggcgaggc gctgctcgtc atccagcgtg acgacgacgc cgcagtgccc catgcacagg
    13681 cggcagaacg acagggcttc gcgcgtggcc atgaccggtg aattcccagg ctgatacgca
    13741 agcgaagata ccgcagcgcc ggcccgcggt ggaaggccgc gcaggcgcct gcgcgcgacg
    13801 gctcagctcg cgccggccgc ccccgtctcg tgcagcagct ccacgccgcc gtgctcgtcg
    13861 acgctctcca ggcgcacgtt gaatccccac agccggtgca ggtgtttcat gacctcgcgg
    13921 taggagtccg ccagcggggt gccctggaac ggggtgtagc gcagtaccag cgagcggtcg
    13981 cctcggatgt ccgtgtccca gatctggatg tcgggctcgt tcatgctcag gttgtactgg
    14041 ttcgagagca cgcggcggat gtgcctgtag ccggcctcgt cgtggatggc gctgatcgtg
    14101 agctcgggcc gcgccggatc gtcggtgacg gcgaacatgc ggaactggcg catcagccgc
    14161 ggcgagagga attgcgccac gaagctctcg tccttgtagt tgcgcatcgc gaaatccagc
    14221 gtcttgcgcc agtcggagcc ggcgatgtcg gggaaccagc ggcggtcctc ctcgtccggg
    14281 tcctcgcaga tgcgcttgat gtcggtgtac atcgcgaagc cgagcgcgta ggggttgatg
    14341 ccagagaagt acgggctgtc gaaggcgggc tggttcacga cgttggtgtg cgagtgcagg
    14401 aactcgagca tgaagccttc gccgatgatg ccctcgtcgt agaggcggtt catgatggtg
    14461 tagtgccaga acgtggccca gccctcgttc atgacctggg tctggcgctg cg
//
LOCUS       DAORUQ010000075        14369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4066_length_14369_cov_6.420078, whole genome shotgun sequence.
ACCESSION   DAORUQ010000075 DAORUQ010000000
VERSION     DAORUQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_14369_cov_6.420078"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLW13_08285"
     CDS             <1..1059
                     /locus_tag="PLW13_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY70410.1"
                     /translation="RYGKDGFRLDVYPTHRNVSQPQWYYDATKQNATGAQLVADGQMI
                     QGNVPGVPFPVPQSGLEVLWNHMIRFQGVQSYYRYDSFYVDANGKPVLAASADNWFEY
                     PMFMEHYKKRNYGSTDLRYAYLRIDYLAPPRRAGEILLVHEPGADYTAGKGRDAWQYL
                     TGQRRVRKAPSVSFDTPNPSTAGTSTYDDSYMYNGSPERFEWKLVGKQEMLIPYNCNT
                     FVFEKPALELLDKHFFKPEYVRWEKHRVWVVEGTLKSGSRHIYGKRRYYIDEDSWTAL
                     LGDLWDGKGELWRVQQMYPFIQWDIPAPGNTALGAYDLKAGIYSINTKPNPGTFTQGF
                     DESEQFFTSQGIARTGVR"
     gene            1170..2276
                     /locus_tag="PLW13_08290"
     CDS             1170..2276
                     /locus_tag="PLW13_08290"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY70411.1"
                     /translation="MKKLAALVCAACLAAPLASAQDTKRYDVLELPAVQSPLAQRAMT
                     YSLTRAGDRVVATGIRGHILYSDDFGHSWVQAESVPVRSSLLDAAFPTPEQGWVVGHE
                     GVILHSTDGGKTWARQLDGVQLAKIGLDFYSARLEQEPDNERYAFLVDELKLALEGNA
                     DRPFFRIVMNSASEGFAAGAYGLLFSTVDGGKSWVPVMERLDLEQFVHLFDFARLPPA
                     GTDNPGPDAVELVAAGEMGTILALDPATGHWQPRDFPYEGSMFTIVATRDASLVTGGL
                     RGLVFRSTDRGASWTEVEKPVTGAIVASTLLADGRVILATQEGMLLASSDDGASFAPL
                     PIEHPQPLSDLIEARPGELVLSGSFGLRVVSLPP"
     assembly_gap    2423..2522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2523..4580
                     /locus_tag="PLW13_08295"
     CDS             <2523..4580
                     /locus_tag="PLW13_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQY70412.1"
                     /translation="SVFYIPGVVREKMKSLWTPNTRYKVVTEEGFEGDSVIPPDYDAS
                     EASIAAVKLNVARAGLGGNLVANDERSAALLVPLMDRNPETGERLDYGALSDALEEVR
                     DKFQAESGGKTQVHIIGFAKIVGDLIAGLKSVVMFFFVAVLIATGLLYWYTRCLRSTL
                     VVIVITMGAVVWQLGLLTVLGFELDPYSILVPFLVFAIGVSHGAQKLNGIMQDTGRGC
                     HPYVAARYTFRRLFLAGFTALASDAVGFAVLLIIQIQVIQDLAITATIGVMVLIFTNL
                     ILIPVTLSYVGVSKSAAEYSHRQLLGDSKPHGITLFLLRFTERKWAIASVGSAVVGSC
                     VALLIAQNLQIGDLDPGAPELRPDSRYNRDMQFMIDHYSVSSDIFAVIVKTEPDKCTD
                     PETLALAEELEWRFRQLPEVEDVVGMNTGARRLLAGLNEGYIKWQSLNRNQSSINYAA
                     VEMVDMGLVNPECSVWPMLVYLADHKAKTLQNAVDTLEQFNRDWPSDKVQFLGAAGNA
                     GFEAATNIVVKQANREMMFYVYAAVIVLSYITFRSVAGVLCAVLPLVATSILCEALMV
                     ILGIGVKVATLPVIALGVGIGIDYALYVLTVVLAKTRAGLPLRDAYYAALTFTGRVVA
                     LIGVTLAAGVITWAFSPVKFQADMGILLAFMFLWNMVGALVLMPSLAYFLLPKPKEAA
                     THA"
     gene            4573..6108
                     /locus_tag="PLW13_08300"
     CDS             4573..6108
                     /locus_tag="PLW13_08300"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQY70413.1"
                     /translation="MREMPLPDVGLGAMILRRAALVPELPAISFEGETLRYAAFGQRI
                     RRLAGALRKAGVNRGDRVAFLGHNHPVFLETLFAATAIGAIFVPLNFRLTGPELEFII
                     NDAGVHTLLVDDAHKAVIDAIRGALCCRHYLAAESACGAWTGLEGFVAHVVPLAAAER
                     VEADDVALIMYTSGTTGRPKGAMLTHGNIFWNNVNARFGPENFAGDVSLTAAPLFHIG
                     GLNVTTLIGLQGGGHVVLHRSFDPALVLEDIARYRVTTMFGAPAMFLFMAQHPGFAGA
                     DLSSVDILICGGAPVPEALLRAYQARGIRLCQGYGLTETAPFASFLGSRWIGSKLGSA
                     GQPPLYGDVRVVDAHNHPLAHGERGEVCVRAPNVMKGYWNRPEATRDAIDAEGWFHTG
                     DVGYFDADGFLYLCDRVKDMVITGGENVYPAEVESVLYEHPAIAEVAVIGLPDEKWGE
                     AVTAVVRPVPGGTLTLEELRSFAGEKLARYKLPLRLHLVDEMPRNPAGKVLKYQLREL
                     LNT"
     gene            6168..7061
                     /locus_tag="PLW13_08305"
     CDS             6168..7061
                     /locus_tag="PLW13_08305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HQY70414.1"
                     /translation="MQFGVIPVCDRRFSNDPRWVRGFLAMLEEEGAESVWMPEHVIMA
                     QDYEPRYEYSADGRAPVAPDTMMPDPLEWLSFAAACTERLRLATGVLIGPQHSAAVLA
                     KRLATLDALSGGRLQVGIGIGWQKEEYEAVGVPYADRGRRLDECLDAMRTLWRDDPAT
                     FRGDFVRFERVFCDTKPVQPGGIPFYIGGSSEAAARRAGLRGEGFYPYVISPEDLAAR
                     MATIRATAAGAGRGLEGFSVTVWPASWRPGATFDIGLARAYRDAGAGRLIIAAYEAGD
                     STPEALRALVAGYRDRIVAAL"
     gene            7107..8591
                     /locus_tag="PLW13_08310"
     CDS             7107..8591
                     /locus_tag="PLW13_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70415.1"
                     /translation="MKGIDPRTPVLVGIGIVEQKEKDPAKAREAVQLMIDAVRAAGAD
                     CGAPLLPAEAQRIYVPQGLWGYADPARMIARAIGAEGARTVYAKVGILQQTLIGDACA
                     RIAAGEIETAIVVGGEARFRALQAQIAGTEAAETPFDEAPDEVLTPQEELQLPLEIDS
                     GLGMMPVGYYALVESAFRAAQGLGVAEHRDRLAAMYSRFSEIAADNPHAWKRERLAPA
                     EIRDATPRNRMLAFPYTRLHNTSWNVDQAAALLLCPAARAEALGIARSRWVFPRASTE
                     SNHMLALSERPALHRLPGARIAGQRALELSGLAPGALDFVELYSCFPIAVELYAAELG
                     IPADRDWTVTGGMPFAGGPLNNYVLQATARMAELLRDKPGSSGLVSSVSGYLTKQGFG
                     VWSAEPGPRGFVFADVSEEVAAESPPRVVVPPADGVARICGYTVMHHNDQRVCGVALL
                     DLPDGTRTLANTLDQALMDRLESEECCHREAQLAGGRFSLDREP"
     gene            complement(8600..9121)
                     /locus_tag="PLW13_08315"
     CDS             complement(8600..9121)
                     /locus_tag="PLW13_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY70416.1"
                     /translation="MDTARTTRSRCGWMLSIALAGAFPAQGATVYRTVDAEGKVSFSD
                     TPPPPGSATEVIEMRDTHAGPDPEAAERLEQMRATTDRLREDRLAREQERAMPPAPVY
                     PGYPEQAQEDSARRDEAQPWLVPYYPPYRRWHGGGDHRPMPRPPYAAPEPLPSGSGAL
                     SPRGLKARLREAR"
     gene            9286..11451
                     /locus_tag="PLW13_08320"
     CDS             9286..11451
                     /locus_tag="PLW13_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQY70417.1"
                     /translation="MASSAIEYRTVEQCVEAAIARVGKRLMVGTPIGIGKPNQLLNAF
                     YRRALADPQIELTVFTGLSLEKPRPGSEIEERFLGPFIARQFGSYEELDYMAALREGT
                     LPPNVRVHEFYFRAGSMKGVAQAQRDYVSSNYTFASRDLIDRGLNVLVQLVASREVDG
                     RRMLSLSSNTDTSLDLLPWMGEQRARGVPCMAIAQVHADMPFMYNRALVEPECFDAVL
                     ANPEYDKTLFAAPNLSVGVTDFAIGVHTSALLRDGGTLQIGIGSLGDAIVYSSRLRHE
                     ENAAYREVIAALGTDAALAECIGGLGRFETGLYGCSEMFVNGFLHLLRAGIVRRRVYD
                     HPGLQRALNQSGSERPDRALLAALRDTGALGSPLAAADVALLKQWGVFDAAVQWREGM
                     LARGASECPADIGDEAALAAVCAFALGEKLLGGIHMHGGFFLGPRDFYEALAAMPREE
                     SELIGMDSVRRINRIDEPVLRELQRRDARFVNTGMMVTLSGAVVSDGLESGEVVSGVG
                     GQYNFVAQAHELPGARSIICIRATRGAGKALRSNIVPHYGHITIPRHLRDIVVTEYGA
                     ADLRGRSDQEIIQALLGIADSRFQPELLEAARASGKIDPHWEIPESRRHNLPGRIAAA
                     LGPWRERGYFPAFPLGTDFTPEEIALGGSLKDIKALMEQPAHLLRAVIRSFMTEVDEE
                     QARPYLERIGLAHPDTPKEKLLQHLLLLELEERGVLRPM"
     gene            complement(11467..11745)
                     /locus_tag="PLW13_08325"
     CDS             complement(11467..11745)
                     /locus_tag="PLW13_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HQY70418.1"
                     /translation="MSRIYIQRDHELDNRTLRKRAERLAKQLQSEFGGNYRWEGNTVH
                     YNYSGGIDARLTLQDADILVDVKLGVLMLMLKKRLHQEIEGYLDEHLA"
     gene            11862..13433
                     /locus_tag="PLW13_08330"
     CDS             11862..13433
                     /locus_tag="PLW13_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HQY70419.1"
                     /translation="MRQRTDRQSSSIFEMPLTGRLVIRPAMRALAEVLPDFAQLGRRN
                     RRLARLEQALESAASYGAWREAAMSYDKEAGLEEWKFTDASPFYDFKLIQRRLAQILG
                     AREGGYQRRLMFILQEGLHGNLGNISNPMLYRFTRFGTKRLIEHYLDEVCEALDWLCD
                     DESGEITLAEKIEFFEATSQTFGQSSLMLSGGAALGIYHMGVVKSLWENGLLPHVISG
                     SSAGSIVAAMLGTHSDEELREVMAHRESLVGLIKWNSPPRTHLFDVEHFNVKLKERIG
                     EMSFQEAFRHSGRAINITVSAYDPLDEGRLLNYRTSPNVVINSAVRASCAAPFLLPPV
                     ELLGKTINGEIIPYLKNRKFLDGSIGDDLPIRRLTRLYGVNHSIVSLVNPLVLPFASR
                     SAHQGNDVSSMMRQYATRLFKETTNFSMQLVQKGIPFDNVNFAIDKLRSVLIQDYRGD
                     ITLVPPRSPAHVFKLFSDQSHAEETEFVAIGARITWPYLEMIKNTTAVSRTFRACLDR
                     LHARAQSAPRARGRK"
     gene            13453..13626
                     /locus_tag="PLW13_08335"
     CDS             13453..13626
                     /locus_tag="PLW13_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQY70420.1"
                     /translation="MSVYRKWECVICGFIYDEALGLPDKGIAPGTRWEDVPEDFECPD
                     CGISKFDFDLLQE"
     gene            13648..14367
                     /locus_tag="PLW13_08340"
     CDS             13648..14367
                     /locus_tag="PLW13_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HQY70421.1"
                     /translation="MPEWALRFENFSALHLATVLFFAALWLALVVVGRRVAGGPLAAR
                     WRAWLCASMALAWLAANGGQLLPSVYVASENLPLHICDIAGILAPLALWRRTRLLLGV
                     LYFWGLGFSVQAIATPDLHQGPASIAFWMFWIPHANITGAAVYALVVEGFRPRWRECA
                     QAYALALLYLALILPFDLLSGFNYGYVGPVTLPQPTLLDLLGPWPWRIGAMMAAALAG
                     FILLQLPWVMASRGADNRVSI"
BASE COUNT         2226 a         4781 c         4922 g         2340 t
ORIGIN      
        1 cgctacggca aggacggttt ccggctcgat gtctacccga cgcaccgcaa cgtctcgcag
       61 ccgcagtggt actacgacgc cacgaagcag aacgcgaccg gcgcccagct ggtcgccgac
      121 gggcagatga tccagggcaa cgtgccgggc gtgcccttcc cggtcccgca gagcggtctc
      181 gaagtgctgt ggaaccacat gatccggttc cagggtgtgc agtcctatta ccgctacgac
      241 agcttttacg tcgacgccaa cggcaagccc gtgctcgcgg ccagcgccga caactggttc
      301 gaatacccca tgttcatgga gcactacaag aagcgcaact acggctcgac cgacctgcgc
      361 tacgcctacc tgcgcatcga ctacctggcg ccgccgcgcc gtgccggcga gatcctgctg
      421 gtgcacgagc ccggcgcgga ctacacggcc ggcaagggac gcgatgcatg gcagtacctg
      481 acggggcagc gccgcgtgcg caaggcgccc tcggtgtcct tcgacacgcc gaacccctcc
      541 accgcgggca cctcgaccta cgacgactcc tacatgtaca acggctcgcc ggagcgtttc
      601 gagtggaagc tggtcggcaa gcaggaaatg ctgatcccct acaactgcaa caccttcgtg
      661 ttcgagaagc cggccctgga actgctggac aagcacttct tcaagcccga gtacgtgcgc
      721 tgggagaagc accgcgtctg ggtcgtcgag ggcaccctga agagcggcag ccgccacatc
      781 tacggcaagc gccgctatta catcgacgag gacagctgga cggcgctgct cggagacctg
      841 tgggacggca agggcgagtt gtggcgcgtg cagcagatgt acccgttcat ccagtgggac
      901 atcccggcgc ccggcaacac ggcccttggc gcgtatgacc tgaaggcggg tatctacagt
      961 atcaatacca agccgaaccc gggcaccttc acgcagggtt tcgacgagtc cgagcagttc
     1021 ttcacatcgc agggcatcgc gcgcacgggc gtgcgctgag ctgtgcggcg cgccgctcgc
     1081 ccgtcgcggc gggcggcgcg cgcgcggtgc tgatgtcgcc gggacgcgtg atgcgggccg
     1141 gggcctggtt tcggggagca acaagaatca tgaaaaaact cgccgccctc gtctgcgcag
     1201 cctgcctggc ggcaccgctg gccagtgcgc aggacacgaa gcgctacgat gtgctcgaac
     1261 ttcccgcggt gcagtccccg ctggcgcagc gcgcgatgac gtattcgctc acccgcgcgg
     1321 gtgatcgtgt cgtcgccacc ggcatccgcg ggcacatcct ctactcggac gatttcgggc
     1381 atagctgggt ccaggccgaa tcggtgccgg tgcgttcctc cctgctcgac gcggcgttcc
     1441 cgacgccgga gcagggctgg gtcgtcggac acgaaggggt gatcctgcac agcaccgacg
     1501 gcggcaagac gtgggccagg caactcgacg gggtgcagct cgcgaagatc gggctggact
     1561 tctacagcgc caggctcgaa caggagcccg acaacgagcg ctacgccttc ctggtcgatg
     1621 agttgaaact cgcgctcgag ggcaacgccg accgtccctt cttcaggatc gtgatgaaca
     1681 gcgcgagcga aggcttcgcg gctggtgctt acggcctgct gttctccacg gtcgacggcg
     1741 gaaagtcctg ggtgccggtg atggaacggc tggatctcga gcagttcgtg cacctgttcg
     1801 acttcgcgcg gctcccgccg gcgggcaccg acaatccggg ccccgacgcg gtggagctcg
     1861 tggcggcggg agagatgggc actatcctcg cgctggaccc cgcgacaggg cactggcagc
     1921 cgcgcgattt cccctacgag ggcagcatgt tcacgatcgt cgcgacccgc gacgcgtcgc
     1981 tggtgaccgg gggactgcgt ggcctcgtgt tccgctccac cgatcgcggc gcgagctgga
     2041 ccgaggtgga gaagccggtg accggcgcga tcgtcgcgag cacgctgctt gccgacgggc
     2101 gcgtgatact ggcgacccag gaaggcatgc tgctcgcgag ctcggacgac ggggcctcgt
     2161 tcgcgccgtt gcccatcgag cacccccagc cgctcagcga cctgatcgag gcacgtccgg
     2221 gagagctcgt gctgagcggg agtttcgggc tacgggtggt atccctgccc ccgtagcacc
     2281 gcccggcccc cgcgggaccg tcacggcata attacaagag gtgcatttgc atggcgcatg
     2341 tcgaagacag cacgaacctg cccgtcatca gcgacgtttc gcaattcgac cgcaactcgg
     2401 gctcctggct ggagcggctg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nntcggtgtt ctacattccc ggcgtcgtgc gcgagaagat gaagtcgctg tggacgccga
     2581 acacccgcta caaggtggtg accgaggagg gtttcgaggg cgattcggtg atacccccgg
     2641 attacgacgc ctccgaggcg tcgatcgcgg ccgtgaagct gaacgtcgcg cgcgccgggc
     2701 tgggcggcaa cctggtcgcc aacgacgagc gctccgcggc gctgctggtc ccgctgatgg
     2761 accgcaaccc cgaaaccggc gagcgcctcg attacggcgc cctgagcgat gcgctggaag
     2821 aggtgcgcga caagttccag gccgagagcg gcggcaagac gcaggtccac atcatcggtt
     2881 tcgccaagat cgtcggcgac ctgatcgcgg gcctgaagag cgtggtgatg ttcttcttcg
     2941 tcgcggtgct gatcgcgacc gggctgctct actggtacac gcgctgcctg cgcagcacgc
     3001 tggtggtcat cgtgatcacg atgggcgccg tggtatggca gctcggactg ctgacggtcc
     3061 tcggcttcga gctcgacccg tattcgatcc tggtcccgtt cctggtgttc gcgatcggcg
     3121 tctcgcacgg cgcgcagaag ctcaacggca tcatgcagga caccggccgc ggctgccacc
     3181 cctacgtggc ggcgcgctac accttccggc gcctgttcct ggcaggcttc acggcgctgg
     3241 cctcggacgc cgtgggtttc gcggtgctgc tgatcatcca gatccaggtc atccaggatc
     3301 tcgcgatcac cgcgaccatc ggcgtcatgg tgctgatctt caccaacctg atcctgatac
     3361 cggtgacgct gtcgtatgtc ggcgtgagca agtctgccgc cgagtactcg caccgccagc
     3421 tgctgggcga ttccaagccg cacggcatca cgctgttcct gctgcgtttc accgagcgca
     3481 agtgggcgat cgcatcggtc ggatccgccg tggtcggcag ttgcgtggcg ctcctgatcg
     3541 cgcagaacct gcagatcggc gacctcgacc cgggtgcgcc cgagctgcgc cccgattcgc
     3601 gctacaaccg tgacatgcag ttcatgatcg accactactc ggtatccagc gacatcttcg
     3661 cggtgatcgt gaagaccgag ccggacaagt gcacggaccc ggagacgctg gcgctcgccg
     3721 aagagctcga gtggcgcttc cggcaactgc ccgaggtcga ggacgtggtc ggcatgaaca
     3781 ccggcgcgcg gcggctgctg gcagggctca acgagggcta catcaagtgg cagtcgctca
     3841 accgcaacca gtcatcgatc aactacgccg cggtcgagat ggtcgacatg ggcctggtga
     3901 atcccgagtg ctcggtgtgg ccgatgctgg tgtacctggc tgaccacaag gcgaagacgc
     3961 tgcagaacgc ggtcgatacg ctggagcagt tcaaccgtga ctggccgagc gacaaggtcc
     4021 agttcctcgg cgccgccggc aacgcgggat tcgaggccgc gacgaacatc gtcgtgaagc
     4081 aggccaaccg cgagatgatg ttctacgtct atgcggcggt gatcgtgctg agctacatca
     4141 cgttccgttc ggtcgcgggc gtgctgtgcg cggtgctgcc gctcgtggcg acatcgatcc
     4201 tgtgcgaggc gctgatggtg atactgggca tcggcgtcaa ggtcgcgacg ctgccggtga
     4261 tcgccctcgg cgtgggcatc ggcatcgact atgcgctgta cgtgctgacg gtggtgctgg
     4321 cgaagacgcg cgcggggctg ccgctgcgcg atgcctacta cgctgccctg acgttcaccg
     4381 gccgggtcgt cgcgctcatc ggcgtcacgc tcgccgccgg cgtcatcacc tgggccttct
     4441 cgccggtgaa gttccaggcc gacatgggga tcctgctggc cttcatgttc ctgtggaaca
     4501 tggtcggcgc gctggtgctg atgccctcgc tcgcgtactt cctgctgccc aagcccaagg
     4561 aggctgcgac gcatgcgtga gatgccgctg cccgacgtcg gcctcggggc gatgatcctg
     4621 cgccgggcgg cgctggtgcc ggagctgccg gcgatcagtt tcgaaggcga gacgctgcgt
     4681 tacgcggcct tcgggcagcg tatccgccgc ctggctggcg cgctgcgcaa ggcgggcgtc
     4741 aaccgtggtg accgtgttgc gttcctcggc cacaaccacc cggtattcct cgagacgctg
     4801 ttcgcggcaa cggcgatcgg cgcgatcttc gtgccgctga atttccgcct caccggcccg
     4861 gagctggagt tcatcatcaa cgacgccggg gtgcacacgc tgctggtcga tgacgcgcac
     4921 aaggcggtga tcgatgcgat ccgcggtgcg ctctgctgcc gtcactacct ggccgcggag
     4981 tccgcgtgcg gggcgtggac agggctcgag ggcttcgtcg cccacgttgt gccgctcgcc
     5041 gcggccgaac gcgtcgaggc cgacgacgtg gcgctgatca tgtacacctc gggcaccacc
     5101 gggcggccca agggcgcgat gctgacgcac ggcaatatct tctggaacaa cgtcaacgcg
     5161 cgcttcgggc cggagaattt cgcgggcgac gtgagcctca ccgccgcgcc cctgttccac
     5221 atcggcgggc tcaacgtcac cacgctgatc gggctgcagg gcggcgggca cgtggtgctg
     5281 caccgcagct tcgatccggc actggtgctg gaggacatcg cgcgctatcg cgtcacgacg
     5341 atgttcggcg cgcccgcgat gttcctgttc atggcgcagc accccggctt cgccggcgcg
     5401 gatctctcca gcgtggacat cctgatctgc ggcggcgcgc cggtgcccga ggcgctgctg
     5461 cgcgcctacc aggcgcgcgg catccgcctg tgccagggtt acggcctcac cgagaccgcg
     5521 ccctttgcct cgttccttgg cagccgctgg atcgggtcca agctcggctc ggcggggcag
     5581 ccgccgctct acggcgatgt gcgcgtggtg gacgcgcaca accacccgct cgcgcacggc
     5641 gagcgcggcg aggtctgcgt gcgcgcgccc aacgtcatga agggctactg gaaccgcccg
     5701 gaagccaccc gtgacgccat agacgccgag ggctggtttc acaccggcga cgtcggctac
     5761 ttcgatgccg acggtttcct gtacctgtgc gaccgcgtca aggacatggt catcacgggc
     5821 ggcgagaacg tctatccggc cgaggtcgag agcgtgctct acgagcaccc ggcgatcgcc
     5881 gaggtcgcgg tgatcggcct tcccgacgag aagtggggcg aggccgtcac cgcggtggtg
     5941 cgcccggtcc ccggcggcac gctcacgctg gaggaactgc gcagtttcgc gggcgagaag
     6001 ctcgcgcgct acaagctgcc gctgcggctg catctcgtcg acgagatgcc gcgcaacccc
     6061 gccggcaagg tgctcaagta tcagctgcgc gagctgctga acacgtaggt ccgcagtcat
     6121 gcggacattg ccgccgtccg gatcaattga cccgcaggga gaggacgatg caatttggcg
     6181 tgataccggt ctgcgaccgg cgcttcagca acgacccgcg ctgggtgcga ggcttcctcg
     6241 cgatgctgga agaggagggc gccgaatcgg tgtggatgcc cgagcacgtg atcatggccc
     6301 aggactacga gccgcgctac gagtattccg cggacggccg cgcgccggtg gctcccgaca
     6361 ccatgatgcc ggacccgctc gagtggctga gcttcgcggc tgcctgcacg gagcgcctgc
     6421 gcctcgccac cggcgtgctg atcggcccgc agcacagcgc ggcggtgctg gccaagcgcc
     6481 tggccacgct cgatgcgctc tcgggcggac gcctgcaggt tggcatcggc atcggctggc
     6541 agaaagagga atacgaggcc gtcggcgtgc cttatgccga ccgcggaagg cggctcgacg
     6601 aatgcctcga cgccatgcgc acgttgtggc gcgacgaccc ggccactttt cgcggcgact
     6661 tcgtgcgctt cgagcgggtg ttctgcgaca ccaagcccgt tcagcccggc ggcatcccgt
     6721 tctacatcgg cggcagctcc gaagccgcgg cgcgccgcgc cgggctgcgc ggggagggct
     6781 tctatcccta cgtgatctcg ccggaggatc tcgcggcgcg catggcgacg atacgcgcca
     6841 ccgctgccgg ggccggacgc ggcctcgagg gcttttcggt cacggtgtgg cccgcgagct
     6901 ggcgtcccgg cgcgactttc gacatcgggc tcgcgcgcgc ctaccgcgac gccggcgccg
     6961 ggcgcctgat catcgccgcc tacgaggccg gcgacagcac ccccgaggcg ctgcgcgcgc
     7021 tggtggcggg ctaccgcgac cgcatcgtcg ccgcgctctg agcgcggccg tccatccgct
     7081 catcgaccag cagcaaggga gcagcaatga agggaatcga tccgcgcacg ccggtgctcg
     7141 tgggcatcgg catcgtcgag cagaaggaaa aggatcccgc gaaggcgcga gaggcggtgc
     7201 agctgatgat cgacgccgtg cgcgcggccg gcgccgactg cggcgcaccg ctgctgccgg
     7261 ccgaggcgca gcgcatctac gtgccgcaag ggctctgggg ctatgcggac ccggcgcgga
     7321 tgatcgcgcg cgcgatcgga gccgagggtg cgcgcaccgt ctatgcgaag gtcggcatcc
     7381 tgcagcagac gctgatcggc gatgcctgcg cgcgcatcgc ggcaggcgag atcgagaccg
     7441 ccatcgtggt ggggggcgag gcgcgctttc gcgcactgca ggcacagatc gcgggcaccg
     7501 aggccgccga gacgcccttc gacgaggcgc ccgacgaggt gctgacgccg caggaggaac
     7561 tgcagctgcc gctggagatc gactcgggcc tgggcatgat gccggtgggc tattacgcgc
     7621 tcgtggagag tgcctttcgc gccgcgcagg gcctcggcgt ggccgagcac cgcgaccgcc
     7681 tggcggcgat gtacagccgc ttcagcgaga tcgccgcgga caacccgcac gcctggaagc
     7741 gcgagcgcct cgcgccggcg gagatccgcg acgccacgcc gcgcaaccgc atgctggcct
     7801 ttccgtacac ccggctgcac aacacctcgt ggaacgtgga ccaggccgcg gcgctgctgt
     7861 tgtgcccggc ggcgcgcgcc gaggcgctcg gcattgcgcg cagccgctgg gtgtttccgc
     7921 gcgcgagcac ggaatcgaac cacatgctcg ccctgtccga gcgccccgcg ctgcaccgcc
     7981 tgccgggggc gcgcatcgcc ggacagcgcg cgctggagct gtccgggctc gcgcccgggg
     8041 cgctcgactt cgtcgagctg tattcctgct tcccgatcgc ggtggagctc tacgccgcgg
     8101 agctcggcat cccggccgat cgcgactgga ccgtgaccgg cggcatgccc ttcgcgggcg
     8161 ggccgctgaa caactacgtg ctgcaggcca ccgcgcgcat ggccgagctg ctgcgtgaca
     8221 agcctggttc gagcgggctg gtgagctcgg tgtcgggata cctgaccaag cagggattcg
     8281 gcgtgtggtc cgccgagccg ggcccgcgcg gcttcgtgtt cgcggacgtg agcgaggagg
     8341 ttgccgcaga atcaccgccg cgcgtggtgg tgcctcccgc ggacggcgtg gcgcgcatct
     8401 gcggctacac cgtgatgcat cacaacgatc agcgcgtgtg cggcgtggcg ctgctggacc
     8461 tgcccgacgg gacgcgcacc ctggccaata cactggacca ggcactgatg gaccggctcg
     8521 agagcgagga atgctgccac cgcgaggcgc aacttgccgg cggacgcttc agcctggacc
     8581 gagagcctta ggtcagcgct cagcgcgcct cgcgcaggcg cgccttcaat ccgcgcgggc
     8641 tgagcgcgcc gctgcccgag ggcagtggtt cgggtgcggc atacggggga cgtggcatcg
     8701 ggcgatggtc gccaccaccg tgccagcgcc ggtagggcgg gtaatacggc accagccagg
     8761 gttgcgcctc atcgcgccgc gcgctgtctt cctgagcctg ttcggggtat ccggggtaga
     8821 cgggagccgg tggcatcgcg cgctcctgct cgcgcgccag gcggtcctcg cgcaagcggt
     8881 cggtggtcgc gcgcatctgc tccagccgtt cggccgcctc gggatcgggg ccggcgtggg
     8941 tgtcgcgcat ttcgatgacc tcggtggcgg agcctggcgg aggcggcgtg tcggaaaaac
     9001 tgaccttgcc ttcggcatcg acggtcctgt agacggtcgc gccctgtgcc gggaatgcgc
     9061 cggccagcgc gatggagagc atccagccgc accgtgaccg cgtggttcgc gccgtgtcca
     9121 tgtcgatctc ctcgcccttt gccgttcgac cgcttcgcag cccaggaatc cgcccggcgg
     9181 gctgatccgg atcaagcgcc ggcacgatcg cgagccgggc cggttgccgt gtcgatgcgc
     9241 ccgactctac tatcgacgca ccatcatgtc agcaggggca gtgtcgtggc gtcgtcagca
     9301 atcgagtacc gcacggtgga gcaatgcgtg gaagccgcca tcgcgcgcgt cggcaagcgc
     9361 ctgatggtgg ggacgcctat cggcatcggc aagccgaacc agctgctcaa cgccttctat
     9421 cggcgcgcgc tcgccgatcc acagatcgag ctcacggtgt tcaccggcct gtcgctggag
     9481 aagccgcggc cggggtccga gatcgaggag cgcttcctcg gaccgttcat cgcacggcag
     9541 ttcggcagct acgaggaact ggactacatg gcggcgctgc gcgagggcac cctgccgccc
     9601 aacgtgcgcg tgcacgagtt ctacttccgc gccgggtcga tgaagggtgt ggcgcaggcg
     9661 cagcgcgact acgtctccag caactacacc ttcgcctcgc gcgacctgat cgatcgcggc
     9721 ctgaacgtgc tggtgcagct ggtggccagc cgcgaggtcg acggccggcg catgctgagc
     9781 ctgtcctcga acaccgacac ctcgctcgac ctgctgccgt ggatgggcga gcagcgcgcg
     9841 cgcggcgtgc cgtgcatggc catcgcgcag gtccacgccg acatgccctt catgtacaac
     9901 cgcgcgctcg tcgaaccgga gtgcttcgac gccgtgctgg ccaatccgga atacgacaag
     9961 acgctgttcg cggcgcccaa tctctcggtc ggcgtcaccg acttcgcgat cggcgtgcac
    10021 acgagcgcgc tgctgcgcga cggcggcacg ctgcagatcg gcatcggctc gctcggcgac
    10081 gccatcgtct actcgagccg gctgcgccac gaggagaatg ccgcgtaccg cgaggtgatc
    10141 gcggcgctcg gcacggatgc cgcacttgca gaatgcatcg gcggcctggg gcgtttcgag
    10201 acgggtcttt acggctgcag cgagatgttc gtcaacggtt tcctgcacct gctgcgcgcg
    10261 ggcatcgtca ggcgccgggt ctacgaccat ccgggcctgc agcgcgcgct gaaccagagc
    10321 ggttcggagc gccccgaccg ggcgttgctc gcggcgctgc gcgacaccgg cgccctcgga
    10381 tcgccgctcg cggcagccga cgtcgcgctg ctgaagcagt ggggcgtgtt cgatgccgcg
    10441 gtgcagtggc gcgagggcat gctggcgcgc ggcgcgagcg agtgcccggc cgatatcggc
    10501 gatgaggccg cgctcgcggc cgtgtgcgca ttcgcgctcg gcgagaagct gctcgggggc
    10561 atccacatgc acggggggtt cttcctgggg ccgcgcgatt tctacgaggc gcttgccgcg
    10621 atgccgcgcg aggagtccga gctgatcggc atggacagcg tgcggcgcat caaccgcatc
    10681 gacgagccgg tgctgcgcga gctgcagcgg cgcgatgcgc gcttcgtcaa taccggcatg
    10741 atggtcacgc tgagcggcgc cgtggtctcc gacggactgg agagcggcga ggtcgtcagc
    10801 ggcgtcggcg ggcagtacaa cttcgtcgcc caggcccacg agctgcccgg tgcgcgctcg
    10861 atcatctgca tccgcgccac gcgcggggca ggcaaggcgc tgcgttcgaa catcgtgccg
    10921 cactacggcc acatcacgat cccgcgtcac ctgcgcgaca tcgtggtcac cgagtacgga
    10981 gcggcggacc tgcgcgggcg cagcgaccag gaaatcatcc aggcgctgct cggcatcgcc
    11041 gattcgcgtt tccagcccga gttgctggag gccgcgcgcg cgagcggcaa gatcgatccg
    11101 cactgggaga tccccgagtc ccggcgccac aacctgcccg ggcgaatcgc cgcggcgctc
    11161 ggcccgtggc gcgagcgcgg ctattttccg gcgtttccgc tgggcaccga cttcacgccc
    11221 gaggagatcg cgctgggtgg cagcctgaag gacatcaagg ccctgatgga acagcccgcg
    11281 cacctgttgc gcgccgtgat ccgctcgttc atgaccgagg tggacgagga gcaggcgcgg
    11341 ccttacctcg agcggatcgg gctggcgcat ccggacacgc cgaaggaaaa gttgctgcag
    11401 cacctgctgc tgctggaact ggaggaacgc ggggtgttgc gcccgatgtg aggagggggc
    11461 tggcgctcag gcgaggtgct cgtcgaggta accctcgatt tcctggtgca ggcgcttctt
    11521 cagcatcagc atcagcaccc cgagcttcac gtcgaccagg atgtccgcat cctgcagcgt
    11581 caggcgcgca tcgatcccgc cggaatagtt gtagtggacg gtattgcctt cccagcggta
    11641 atttcccccg aactcgctct gcaactgctt ggccagccgc tcggcgcgct tgcgcagcgt
    11701 ccggttgtcg agctcgtgat cgcgctggat gtagatgcgg ctcattccct gttccccggc
    11761 cctgattcac atcaagattg tgcgccgtcg gcgggcgctt gccaatcgcc cggacgtgac
    11821 ataataagct catcgtgatg tacccccggg gaggcagcaa attgcgccag cgaacggaca
    11881 ggcaatcgtc atccatattc gagatgccgc tgacgggccg gctggtgatc cgtccggcga
    11941 tgcgtgcgct ggcagaggtc ctgccggact tcgcccagct cggacggcgc aaccgccgcc
    12001 tggcgcgcct ggagcaggca ctggaaagtg ccgccagtta cggcgcctgg cgcgaggcgg
    12061 cgatgagcta cgacaaggag gcggggctgg aggagtggaa attcaccgat gcctcgccgt
    12121 tctatgactt caaactgatc cagcgccgcc tggcgcagat cctgggcgcg cgcgagggcg
    12181 gctaccagcg ccgcctgatg ttcatcctgc aggagggcct gcacggcaac ctcggaaaca
    12241 tctccaaccc gatgctgtac cgcttcacgc gtttcggcac caagcgtctg atcgagcact
    12301 acctcgacga agtctgcgaa gcgctggact ggctctgcga tgacgaatcg ggcgagataa
    12361 cgctcgcgga gaagatcgag ttcttcgagg ccaccagcca gaccttcggc cagtccagcc
    12421 tgatgctcag cggcggcgcc gcgctcggta tctaccacat gggcgtggtg aagtcgttgt
    12481 gggagaacgg cctgctgccg cacgtgatct cgggttccag cgccggctcg atcgtcgccg
    12541 cgatgctcgg cacgcacagc gacgaggagc tgcgcgaggt gatggcgcac cgcgaatcgc
    12601 tggtcgggct gatcaagtgg aactccccgc cgcgcaccca cctgttcgac gtcgagcatt
    12661 tcaacgtcaa gttgaaggag cgcatcggcg agatgagctt ccaggaggcc ttccgccaca
    12721 gcggccgcgc gatcaacatc acggtctcgg cctacgaccc gctcgacgag ggccggctgc
    12781 tgaattaccg cacctcgccg aacgtggtga tcaacagcgc agtgcgcgcc tcctgcgccg
    12841 caccgttcct gctgccgccc gtggaactgc tgggcaagac catcaacggc gagatcatcc
    12901 cctacctgaa gaaccgcaag ttcctcgatg gatcgatcgg cgacgacctt ccgatacgcc
    12961 gcctgacgcg actctacggc gtgaaccact cgatcgtgag cctggtgaac ccgctggtgc
    13021 tgcccttcgc ttcgcgcagc gcgcaccagg gcaacgacgt ctcctcgatg atgcggcaat
    13081 acgcgacgcg cctgttcaag gaaaccacca atttctcgat gcagctggtg cagaagggca
    13141 ttccgttcga caatgtcaac ttcgcgatag acaagctgcg ctcggtgctg atccaggact
    13201 accgcggcga catcacgctg gttccgccgc gcagtccggc gcatgtattc aagctgttca
    13261 gcgaccagtc gcacgccgag gagacggagt tcgtggcgat cggcgcgcgc atcacctggc
    13321 cctacctcga gatgatcaag aacaccacgg cggtgagccg gaccttccgc gcctgcctcg
    13381 accggctgca tgcgcgcgcg cagagcgcgc cgcgcgcccg cgggcgcaaa tgatttctgg
    13441 atggagtgat acatgagcgt ctacaggaaa tgggaatgcg tgatctgcgg cttcatctac
    13501 gacgaagccc tcggcctgcc ggacaagggc atcgcgccgg gcacgcgctg ggaagacgtt
    13561 cccgaggatt tcgagtgccc ggactgcggc atcagcaagt tcgatttcga cctgctgcag
    13621 gagtgaggcg cgcgccgggc acccgcgatg cctgagtggg cgctgcgctt cgagaatttc
    13681 tccgcgctgc acctcgcgac ggtactgttc tttgccgcgc tgtggctggc gctggtcgtc
    13741 gtcgggcggc gggtggcggg cggcccgctc gcggcgcgct ggcgggcgtg gctgtgcgcc
    13801 tcgatggcgc tggcctggct cgcggccaac ggcgggcaac tgctgccgtc ggtttacgtg
    13861 gcctcggaga acctgccgct gcatatctgc gacatcgcgg gcatcctggc gccgctggcc
    13921 ttgtggcggc gcacccggct gctgctcggg gtgctgtatt tctgggggct gggattcagc
    13981 gtgcaggcga tcgcgacgcc cgatctgcac cagggtccgg ccagcatcgc gttctggatg
    14041 ttctggatac cgcacgccaa catcaccggt gcggccgtct atgcgctcgt ggtggagggg
    14101 ttccggccgc gctggcgcga atgcgcgcag gcctacgcgc tggcgctgct gtacctcgcg
    14161 ctgatcctgc ccttcgatct gctcagcggc ttcaactacg gctacgtggg cccggtgacc
    14221 ctgccgcaac ccacgctgct cgacctgctc ggcccctggc cctggcgcat cggcgcgatg
    14281 atggccgccg cactggccgg cttcatcctg ctgcagctgc cgtgggtgat ggcgtcacgc
    14341 ggagccgaca accgggtgtc gatctgatc
//
LOCUS       DAORUQ010000076        14359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4068_length_14359_cov_6.425545, whole genome shotgun sequence.
ACCESSION   DAORUQ010000076 DAORUQ010000000
VERSION     DAORUQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_14359_cov_6.425545"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PLW13_08345"
     CDS             complement(<1..112)
                     /locus_tag="PLW13_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQY70422.1"
                     /translation="MGLGRMGQAIAARLLEANRELIVWNRSAQACDALLER"
     gene            complement(177..515)
                     /locus_tag="PLW13_08350"
     CDS             complement(177..515)
                     /locus_tag="PLW13_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY70423.1"
                     /translation="MSSSAWKAFDLNELKKKVAGTEPRIVEFLRGESLSCAIYRLPAG
                     SKDMQAPHLEDEIYVVLDGRARLRIDEQERDVGAGMVLFVGATTEHSFFDIEEDLTLL
                     AIFGPSDTLV"
     gene            complement(605..1210)
                     /locus_tag="PLW13_08355"
     CDS             complement(605..1210)
                     /locus_tag="PLW13_08355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQY70424.1"
                     /translation="MLTIAPVTLEGHGVRLEPLAPDHAPALAAAAADGRLWELWFTSV
                     PEPGQTIAYVEQALAGQRDGHMLPWAVRDLASGAIVGSTRYHDIVAAIDRLEIGYTWY
                     ARGHQRSHVNTGCKRLLLAHAFETLGCAVVGLRTDNFNFASQRAIAALGARKDGVIRH
                     HASRRDGSVRDTVMFSILATEWPDVKKHLQLRLARHSDRES"
     gene            complement(1282..1956)
                     /locus_tag="PLW13_08360"
     CDS             complement(1282..1956)
                     /locus_tag="PLW13_08360"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HQY70425.1"
                     /translation="MLKSSLSRYLAPLLLALLAGGAYAADLPQTSHDGLQLVPGKVKV
                     LYVKPGVDLSPYQRVAITDAYVAFRKNWEREHKTGALRVSSRDMEKIKTGLAADLRKI
                     FTEELQKGGYEVVQEGGDDVLVLRPAIIDLDVAAPDTMEPGRSRSFATSAGAMTLYLE
                     LYDGASGEILARAVDPKDARDTGRMMWQNSVTNRAEADRMLRKWAVLLHEALDRARAQ
                     QAPAGG"
     gene            2105..2527
                     /locus_tag="PLW13_08365"
     CDS             2105..2527
                     /locus_tag="PLW13_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HQY70426.1"
                     /translation="MNKNNDITAVVGAETDIAPLGRDTPKPAHELTAAEDRDSVDWAA
                     VECSPAFRALLAAKRRFIVPATLFFVVYYFALPLAVGFLPGLMSTRVAGNLNLAYLFA
                     LSQFVMAWTLAALYVRAAARWDRMAADIAATARRIEGS"
     gene            2530..4098
                     /locus_tag="PLW13_08370"
     CDS             2530..4098
                     /locus_tag="PLW13_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HQY70427.1"
                     /translation="MSSTLAMFLGFVAITLAVTWWSARRSSGASAYFAAGRRITGWQN
                     GLAIAGDYMSAASFLGIAGMIAFHGFDGFMYSVGWLVAYLTVLLIVAEPLRNSGKYTM
                     ADVLAYRLSPRPVRAMAALSTLTVSTFYMIAQMVGAGSLVKLLLPGVSYEMAVAAVGA
                     LMIVYVVFGGMLATTWVQIIKAVLLMSGTLFLSVLVLAHFDYSFADFFAAIAAVSWTG
                     ADGVTVTKDFLQPGSMYKPPWGAIELLSLGLALIFGTAGLPHILVRFYTVPDAPTART
                     SVVWAMALIGLFYVMTTFLGFGAATILTPANIAVENMSAPLLAEALGGELFFAFISAV
                     AFATILAVVAGLTISASTSFAHDFYTNVLHHGRERREGEEVRVARITALVVGALAIAL
                     AIRLQSINVAFLVGLAFAVAASANLPVIVLSLFWRRFNTGGAVCGLALGLAGSIVLIA
                     LSPSVMGTGPDALIHHEPLFPLKNPGILSIPLGFLGAVLGTLFTRAQPGAEAAFAELS
                     VRANTGIGAERASP"
     gene            complement(4116..5036)
                     /locus_tag="PLW13_08375"
     CDS             complement(4116..5036)
                     /locus_tag="PLW13_08375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70428.1"
                     /translation="MTTTTQRHAAGELPLAGRSALITGGSGGIGAACAAALLRDGASV
                     TLSARRPELLEATRQRLQPLVREGARLATIAGDSLYRGDVQAAIALACEHTGALDICI
                     PTVGGGDIKPFLLHDETSFLKDIELNVISAFLVIRHATPIMARAGGGSVVCISSEAAK
                     LSFPWLTAYVTAKCALEGMVRALAEELSRYRIRVNAVRPGTTRTEATGMIYADAETYR
                     RFAEEKPLGRLGEPEDIAAGVRYLAGPESAWVTGQSFAIDGGNELRKAADMAPLVEQI
                     YGAEVFAKVLRGEDPLAPGQPDALRPGDEY"
     gene            complement(5237..5608)
                     /locus_tag="PLW13_08380"
     CDS             complement(5237..5608)
                     /locus_tag="PLW13_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70429.1"
                     /translation="MNHEQLMRDYYRTYNSEDPAALRAFYAEDVVLLSAQGELRGPEA
                     ILGTYDYLTSQFRDQMTPTHIAVDGDTAVVAITDRFTAKVDVPDFMGVALKQGESLEL
                     SLRGTYTIADGKFRRILIEAL"
     gene            complement(5713..6819)
                     /locus_tag="PLW13_08385"
     CDS             complement(5713..6819)
                     /locus_tag="PLW13_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY70430.1"
                     /translation="MARAPDRLLDTLYASILDDPPWLAFLEAMEHHVPCHHCTMVLRK
                     PREGDAGVLIASPDNNAAMAALQQRHYRDSPFLELPEGKVCILAERELKARYPEYYGY
                     IRHYSKATDLIGVDLLEPRTGMTFRLRGARIDGEPSFGERERRVFDALVPRLRTAIAL
                     YARIALQQYQLSVLDETAGQLAIGSMVLDDSGRVLVKNMVADRLLLDRDGFHLRAGVL
                     HCNEAKDDRNLRVLLTRFRNDATAMAKEQAIKVRRGNGERFWSVLARPSRARPGLEEK
                     ASATVVVLVRDASHTPDVTDAALIELFGLTRAEAALAVRLVKGESLNDAAVSLGISRY
                     TARVQLASIFSRTGIHRQPQLVSHILGTLNTVWG"
     gene            6952..8418
                     /locus_tag="PLW13_08390"
     CDS             6952..8418
                     /locus_tag="PLW13_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQY70431.1"
                     /translation="MQESMRVPFVLRGEIIDDCTVEHGGRHGQARFLTPDIESALGRL
                     PLREPSRMADLYSLTMDDIVGYLARLGKALDVRTNRHMQEAFALSCRTSGLSEPILRY
                     YYELIPQSFEPAVVREMAERLVGIDYLEGWVEQASALHTGVPTRVRAFGARCVHVIAG
                     NVPMVSVGTVVRNAFTRSDAIIKTPSNDPLTATAIARTMVEMDRDHPLTRHMSVVYWK
                     GGDESVEREIYDPRGVEKIVAWGGFASIKHVTRYLQPGLDLITLDPKHSSTIIGREAF
                     ADDVTLESVARRLALDIGAMNQEGCVNARVVYVQSGTDAEGLARANRLGELAIAALQQ
                     LPEFLSTPHKAFDAALKEEIDGLRYAGDEYVVYGGRKNEGAIIVSQNDAPVDFARQLA
                     CRVGNIVPLDDIDTAVRSVNAYTQTIGIFPETLKTQLRDRLSYQGAQRLVSLGAAALF
                     EDAATPQDGIEPVRRMCKWIVEQTSDPVMLDNLARGTM"
     gene            8478..9896
                     /locus_tag="PLW13_08395"
     CDS             8478..9896
                     /locus_tag="PLW13_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70432.1"
                     /translation="MSELFQNLLRDPQSYIDGDYLAGHAFTREQIEAVHLAGLRKRFN
                     ELRGQVPVLDRLATEQGIDAIDSLEDAVPLLFPHTVYKSYPLSILERGQFDRLTRWLD
                     GLTTIDLGKIDTAGVDSIDGWIKRLDEQTDMRLLHTFGTTGKLSFMPRMREHWIQSTT
                     LTAHAIRDWNGHGTGPDLMSARLPMVQPGYRYGAGATYRGVEAMVKLWAGGDDNALFL
                     YPDDYFSADIASLAGRLRAAEARGEQGRLELSPGLLARREEFAHREQQRPGRMKEFLQ
                     TAIRRFGGRDICLFAVWPILFEWAEQGLAEGMRGLFGTGSLLMSGGGSKGRVLPEDYR
                     EQIFDFLGYRRTFEIYSMSELMPGNPRCEAGNYHVPPVLVPFVLDPQTGKMLPRAGTQ
                     TGRFACFDMLPDTYWGGLVTGDEVTMAGWDEPCDCGRRSPYLHGTIRRFSEIQGGDDK
                     INCAGAPEAHDRAMDFLLHAAG"
     gene            complement(10098..11657)
                     /locus_tag="PLW13_08400"
     CDS             complement(10098..11657)
                     /locus_tag="PLW13_08400"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HQY70433.1"
                     /translation="MDNLRFDNRFVNELPGDPEHGPRLRQVHGALYSRVHPTPVAAPA
                     LIAHSREVAELLGIGEEHIASELFARVFGGNALLEGMAPYAANYGGHQFGNWAGQLGD
                     GRAISLGETVNASGERWELQLKGAGPTPYSRNADGRAVLRSSIREFLCSEAMHHLGVP
                     TTRALCLVGTGEPVVRDMFYDGHPRAEAGAIVCRVAPSFIRFGNFELPASRNDVALLE
                     RLIDFTIARDFPHLRGSREQLHAAWFREVCERTARLMAEWMRVGFVHGVMNTDNLSIL
                     GLTIDYGPYGWIDDFDPNWTPNTTDAGTRRYRFGQQPQVAYWNLGRLASALAPAFGGV
                     EALQEGLQHYVAAWHAAERGNIAAKLGLADCRDEDVTLMEDLRGLLQSAEVDMTIFFR
                     ALDGVDPAAPSLAPFEEAFYDAAKRRGAEGSFLAWLARYGARVGADPLPAADRRERMR
                     RANPRYVLRNYLAQQAIDRAEQGDYAGIHELLEVLRRPYEDQPGREAFAVRRPDWARD
                     RAGCSMLSCSS"
     gene            11852..13324
                     /locus_tag="PLW13_08405"
     CDS             11852..13324
                     /locus_tag="PLW13_08405"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY70434.1"
                     /translation="MTDKKTLRDIRVVVMGAGMAGILSGIKLLQAGITNCAIYEKADR
                     IGGTWRENTYPGLTCDVPSHAYTYSFEPNPDWSHMLPPGAEIQEYFERTTRKYGVDKI
                     IRFNEEIVRCEFRDGRWHLETKNGTRDQADIVIAATGVLHHLNYPDIPGLASFEGACF
                     HTARWDHSVPLDGRRVGVIGNGSTGVQIISALAPRAGKLHHFQRTAQWIMPVENPAFT
                     DEQRAAFRADPALLHYMQNQPDYLANVERFNNSLVNADSEGIAEIEATCLANLENTVR
                     DPVLREKLRPDYRAACKRLIYSPDYYEAIQHPNAELVTDGIECIEPAGVRLKNGRLVE
                     LDVLALATGFNAGMFMRPMNVVGRDGVQLNDVWADRPKAYMAIAMPDFPNFFMLNGPN
                     GPVGNFSLIDIAEQQLHYIWTFIEKLRAGECREVAPTPEAMQAFERDRLEAAKRTVFG
                     SGCKSWYLDAEGIPATWPWTRTRFCEEMKAPRLEAYAFAS"
     gene            13450..13947
                     /locus_tag="PLW13_08410"
     CDS             13450..13947
                     /locus_tag="PLW13_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY70435.1"
                     /translation="MRRLVTGIDADGKSVFVHDGPCPQPVSGAGFELGALWASTPDTR
                     LGESGTDAAIGKTSTVPPPGATVFTYVVIPPDAEAARGQADTVSASADFNVEDDGRMH
                     RTDTIDYGVLLAGELWLELDDGRQQLLKAGDSFVQNGTRHAWHNRGSKPAIMCAVMIG
                     MAREA"
     gene            complement(14066..>14359)
                     /locus_tag="PLW13_08415"
     CDS             complement(14066..>14359)
                     /locus_tag="PLW13_08415"
                     /inference="COORDINATES: protein motif:HMM:NF022885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HQY70436.1"
                     /translation="HASPELKSAHRFREAWSRICAEDALEQAVGRGPENAGPLNSHML
                     VLRSLTLMRELSPDYLRRFLSQVDSLLWLEQAQGKLRQPATRPKPARQRRPGK"
BASE COUNT         2295 a         5015 c         4781 g         2268 t
ORIGIN      
        1 cgcgctcgag cagcgcgtcg caggcctgcg cgctgcggtt ccacacgatc agctcgcggt
       61 tcgcctcgag caggcgcgcc gcgatggcct ggcccatgcg gcccagcccc acgaatccga
      121 tcacctcgtc gccatcgagc acgcgtgcct ctcctgcctt ggtggtcgtc gtggagctac
      181 acgagcgtgt cggaaggacc gaatatcgcg agcagcgtca ggtcttcctc tatgtcgaaa
      241 aaggaatgct cggtggtggc gccgacgaac agcaccatgc cggcaccgac gtcgcgctcc
      301 tgctcgtcga tgcgcagccg cgcgcggccg tcgagcacga cgtagatctc gtcctccagg
      361 tgcggcgcct gcatgtcctt cgatccggcc ggcagccggt agatcgcgca ggagaggctc
      421 tcgccgcgca ggaactccac gatacgcggt tcggtgccgg cgactttctt cttcaattcg
      481 ttgaggtcga aggctttcca agcgcttgag gacatctgga tgatccctgg tgacgggttg
      541 cagaggaaaa cctcacttta atgcgcaagc gcccccggtg ccaacccgcc gcgcagcctt
      601 gcgttcagct ctcgcggtcc gagtggcgcg cgaggcgcag ttgcaggtgc tttttcacgt
      661 cgggccactc ggtggcgagg atgctgaaca tgacggtgtc gcgcaccgag ccgtcgcggc
      721 gcgacgcgtg atggcggatc acgccgtcct tcctggcgcc gagagcggcg atagcgcgct
      781 gcgaggcgaa gttgaagttg tcggtgcgca gccccaccac cgcgcagccg agcgtctcga
      841 aggcatgcgc gagcaacagc cgcttgcagc ccgtgttcac gtgcgagcgc tggtgcccgc
      901 gcgcgtacca ggtgtagccg atctcgaggc gatcgatcgc ggcaacgata tcgtgatagc
      961 gcgtgctgcc cacgatcgca ccgctggcca gatcgcgtac cgcccagggc agcatgtgcc
     1021 cgtcgcgctg cccggccagc gcctgctcca cgtacgcgat cgtctggccg ggctccggca
     1081 cgctcgtgaa ccagagttcc cacagcctgc cgtcggcggc agccgcggcg agcgccggcg
     1141 catgatcggg cgcgagcggt tcgagcctta cgccgtggcc ctcgagcgtc accggcgcga
     1201 tcgtcagcat gcgcatcctc ccctggtgcc gaagcgagaa tcagaacgcg acacccccgg
     1261 tctgcgcacg tcgggagacc ctcacccgcc tgccggcgct tgctgcgcgc gcgcccggtc
     1321 cagcgcctcg tgcagcagaa ccgcccactt gcgcagcatg cggtcggcct cggcgcggtt
     1381 ggtgacggag ttctgccaca tcatgcgccc ggtgtcgcgc gcatccttcg gatcgacggc
     1441 gcgcgccagg atctccccgc tggccccgtc gtagagctcc agatagagcg tcatggcgcc
     1501 cgcgctggtc gcgaagctcc ggctgcggcc cggctccatc gtgtcgggcg cggccacgtc
     1561 gagatcgatg atcgccgggc gcagcacgag tacgtcgtcg ccgccctcct gcaccacctc
     1621 gtagccgccc ttctgcaact cctcggtgaa gatcttgcgc aagtccgcgg cgaggccggt
     1681 cttgatcttc tccatatccc tggagctcac gcgcagggca ccggtcttgt gctcgcgctc
     1741 ccagttcttg cgaaacgcca cgtaggcgtc cgtgatggcg acacgctggt agggcgaaag
     1801 atccaccccc ggcttgacgt agagaacctt caccttgccg ggcacgagtt gcagcccgtc
     1861 gtggctggtt tgcggcagat cggccgcgta cgcgccgccg gccagcaacg cgagcagcag
     1921 cggcgccagg tagcgcgaca acgaagactt gagcatggga acctccccgg gcatcagccc
     1981 tgaccatggt tggagacaac ataccggtaa aacgataacg cagtttcccg acgccacgcc
     2041 atgccctccc cggaacagtc gccgccgcgt ggcaggcgct tgcggcttcg accataatgc
     2101 gggcatgaac aagaacaacg acatcaccgc agtcgtcggc gccgagaccg acatcgcgcc
     2161 gctggggcgc gatacgccga aacccgctca cgagctcacc gcggcggagg accgcgacag
     2221 cgtggactgg gccgcggtgg aatgcagccc cgcctttcgc gcactgctcg ccgcgaagcg
     2281 gcgcttcatc gtgccggcca cgctgttctt cgtcgtctac tacttcgcgc tgccgctcgc
     2341 cgtgggcttc ctgcccgggc tcatgagcac gcgcgtggcg ggcaacctga acctcgccta
     2401 cctgttcgcc ctgtcgcagt tcgtgatggc ctggacgctg gcggcgctct atgtgcgcgc
     2461 cgcggcgcgc tgggaccgaa tggccgcgga catcgccgcc accgcgcgac ggatcgaggg
     2521 gagctgagca tgtcgagcac gctggcgatg ttcctcggtt tcgtcgcgat caccctcgcg
     2581 gtgacctggt ggtcggcgcg ccgctcgagc ggcgccagcg cctatttcgc tgccggacgg
     2641 cgcatcaccg gctggcagaa cggactggcg atcgccggcg actacatgag cgcggcgagc
     2701 ttcctcggca tcgccggcat gatcgccttc cacggtttcg acggcttcat gtactcggtg
     2761 ggctggctgg tcgcctatct cacggtgctg ctgatcgtgg cggagccgtt gcgcaactcc
     2821 ggcaagtaca ccatggccga cgtgctcgcc tatcggctaa gcccgcgacc ggtgcgcgcg
     2881 atggccgcgc tcagcacgct caccgtatcc actttctaca tgatcgcgca gatggtcggc
     2941 gccggttcgc tggtcaagct gctgctgccc ggggtgagct acgagatggc cgtggccgcg
     3001 gtcggcgcgc tgatgatcgt gtacgtggtc ttcggcggca tgctcgccac cacctgggtg
     3061 cagatcatca aggccgtgct gctgatgagc ggcacgctgt tcctgagcgt gctggtgctg
     3121 gcgcacttcg attacagctt cgcggacttc ttcgcggcga ttgccgcggt gagctggacc
     3181 ggcgccgacg gcgtcacggt cacgaaggac ttcctgcaac cgggctcgat gtacaagccg
     3241 ccctggggcg cgatcgagct gctgtcgctg gggctcgcgc tgatcttcgg taccgcggga
     3301 ctgccgcata tcctggtgcg cttctacacc gtgcccgatg cgcccacggc gcgcacctcg
     3361 gtggtgtggg cgatggcact gatcgggctg ttctacgtca tgaccacctt cctcggcttc
     3421 ggcgcggcga ccatcctgac gcccgccaac atcgcggtcg agaacatgag cgcgccgctg
     3481 ctcgccgagg ccctcggcgg cgagctgttc ttcgccttca tcagcgcggt ggcattcgcc
     3541 accatcctcg ccgtggtggc cggcctgacg atcagcgcca gcaccagctt tgcgcacgac
     3601 ttctacacca acgtgctgca tcacggccgc gagcggcgcg agggcgagga agtgcgcgtg
     3661 gcgcgcatca ccgcgctggt ggtcggcgcg ctggcaatcg cgctagcaat ccgcctgcag
     3721 agcatcaacg tcgccttcct ggtgggcctg gcattcgcgg tcgcggccag cgccaacctg
     3781 ccggttatcg tgctctcgct gttctggcgc cgcttcaaca ccggcggcgc cgtgtgcgga
     3841 ctggcgctcg ggctcgcggg atcgatcgtg ctgatcgcgc tcagcccgag cgtgatgggc
     3901 accggaccgg acgcgctgat ccaccatgag ccgctgttcc cgctgaagaa cccgggcatc
     3961 ctgagcattc cgctgggatt cctcggcgcg gtgctgggca cgctgttcac gcgcgcgcag
     4021 cccggtgcgg aggcggcctt tgccgagctg agcgtgcgcg ccaacaccgg catcggcgcc
     4081 gagcgcgcca gcccgtaggt ccggcttcag ccgtatcagt actcgtcccc ggggcgcagc
     4141 gcatccggct gcccgggcgc cagcggatcc tcgcctcgca gcaccttcgc gaacacctcg
     4201 gcgccgtaga tctgctcgac cagcggcgcc atgtcggcgg ccttgcgcag ctcgttgccg
     4261 ccatcgatcg cgaagctctg tcccgtgacc caggcggatt ccggaccggc gagatagcgc
     4321 actcccgccg cgatgtcctc gggctcgccg agccgcccga gcggcttttc ctcggcgaag
     4381 cggcggtagg tctcggcgtc ggcgtagatc atgccggtcg cctccgtgcg cgtggtcccg
     4441 gggcgcacgg cattgacccg gatacggtag cgcgacagct cctcggccag cgcgcgcacc
     4501 atgccctcga gcgcgcactt cgcggtgacg taggcggtga gccacggaaa ggagagtttc
     4561 gccgcctcgg aagagatgca cacgacgctg ccgccgccgg cgcgcgccat gatcggcgtc
     4621 gcgtgccgga tgacgaggaa ggcggatatc acgttcagct cgatgtcctt caggaagctg
     4681 gtctcgtcgt gcagcaggaa gggcttgatg tcgccgccgc cgaccgtcgg gatgcagata
     4741 tcgagcgcac cggtgtgctc gcaggccagc gcgatcgccg cctgcacgtc gccacgataa
     4801 agcgaatcgc cggcaatggt cgcgagccgc gcaccctcgc gcaccagcgg ctgcaggcgc
     4861 tggcgcgtcg cctcgagcaa ctcgggacgg cgcgcggaca gcgtgaccga ggcgccgtcg
     4921 cgcagcagcg cggcggcgca cgcggcgccg atgccgcccg agcctcccgt gatcagagcg
     4981 ctcctgcccg cgagcggcag ttcgcccgcg gcatgtcgtt gcgtggtggt cgtcatcgag
     5041 gcccctcgtc gttgccgtgc ggtgaatatg gccggggatg atcgcgcccg ctacccgcgg
     5101 tggcaagcaa cgcgcgcgac tgcaggcccg gcatcgccgg attcttgtcg gtgtaggtcg
     5161 gcattcatgc cgacgggagc ctgcggggac tcggcgggtt cggctacgcc gcacccttgt
     5221 cgggctgaag cccgacctac agggcctcga tcaggatgcg gcggaacttg ccgtcggcaa
     5281 tggtgtaggt gccgcgcagc gaaagctcga ggctctcgcc ctgcttcagc gcgacgccca
     5341 tgaaatccgg cacatcgacc ttcgccgtga agcggtcggt gatcgcgacc accgcggtat
     5401 ccccgtccac cgcgatgtgc gtgggcgtca tctggtcgcg gaactggctg gtcaggtagt
     5461 cgtaggtgcc gagtatcgcc tccggtccgc gcagttcgcc ctgcgccgag agcagcacca
     5521 cgtcctcggc gtagaaggca cgcaacgccg cggggtcctc gctgttgtag gtgcggtaat
     5581 agtcgcgcat cagttgttcg tggttcatgc cttgccttcc gtattgcttc gatgtccggc
     5641 ccgagccgga cccggatcca ccccgttggt ccgaattcat tcggtcaatg tccggctgaa
     5701 gccggacctg cgtcagcccc acacggtatt cagcgtgccg aggatatggc tcaccagctg
     5761 gggctgccgg tgtatcccgg tgcgagaaaa aatcgaggcc agctggaccc gggcagtgta
     5821 gcgcgagatg ccgagcgaca ccgcggcatc gttgaggctc tcgcccttca ccagccgcac
     5881 cgccagcgcg gcctcggcgc gcgtcagtcc gaacagctcg atcagcgccg cgtcggtcac
     5941 gtccggcgtg tggctggcat cgcgcaccag cacgactacc gtcgccgagg ccttttcctc
     6001 cagccccggg cgggcgcgcg aggggcgcgc cagcacgctc cagaagcgct cgccattgcc
     6061 gcgacgcacc ttgatcgcct gctccttcgc catcgccgtc gcatcgttcc tgaagcgcgt
     6121 gagcagcacg cgcaggttgc ggtcgtcctt cgcctcgttg cagtgcagca cgccagcgcg
     6181 caagtggaac ccgtcgcgat ccagcagcag gcggtccgcg accatgttct tcaccagcac
     6241 gcggccgctg tcgtcgagca ccatcgagcc gatcgcgagc tgccccgcgg tctcgtcgag
     6301 cacgctcagc tggtattgct gcaacgcgat gcgcgcgtag agcgcgatcg cggtgcgcag
     6361 gcgcgggacc agggcatcga acaccctgcg ttcgcgctcg ccgaaggacg gctcgccgtc
     6421 gatgcgcgcc ccgcgcagcc ggaaggtcat gccggtgcgc ggctcgagca gatccacgcc
     6481 gatcaggtcg gtcgccttgc tgtagtgcct gatgtacccg tagtactcgg ggtatctggc
     6541 cttcagctcg cgctcggcaa ggatgcagac cttgccctcg ggcagctcca ggaacggcga
     6601 atcgcggtag tggcgctgct gcagcgcggc catcgcggcg ttgttgtcgg gactcgctat
     6661 cagcacgccg gcatccccct cgcgcggctt gcgcagcacc atggtgcagt gatggcaggg
     6721 cacgtggtgc tccatcgcct cgaggaacgc gagccacggc gggtcgtcga gaatgctcgc
     6781 gtagagcgtg tcgagcagcc gatccggtgc gcgcgccatg ggtgagccgt tccgtttgcc
     6841 tgaatctgtc gcgccagatc gtagtacatt tgacggatgt cggggcagcc cgccgtgcgc
     6901 atactgaccg ggagccacaa gacaccgaca ccggccagga ggagtcatcc catgcaggaa
     6961 tccatgcgcg tcccgttcgt gctgcgcggc gagatcatcg acgactgcac ggtggagcat
     7021 ggcggccgcc acggccaggc ccgcttcctc acgcccgaca tcgaatccgc gctcggcagg
     7081 ctgccgctgc gcgaaccctc gcgcatggcc gacctctact cgctcacgat ggacgacatc
     7141 gtcgggtatc tcgcgcgcct cggcaaggcg ctcgacgtgc gcaccaacag gcacatgcag
     7201 gaagccttcg cgttgtcgtg ccgcacctcg ggcctgtccg aaccgatcct gcgctactac
     7261 tacgaactga tcccgcagtc cttcgaacct gccgtggtac gcgagatggc cgagcggctg
     7321 gtcggcatcg actacctcga gggctgggtg gaacaagcct ccgcgctgca caccggcgtt
     7381 ccgacgcgcg tgcgtgcctt cggggcgcgc tgcgtgcacg tgatcgcggg caacgtgccg
     7441 atggtcagcg tgggcacggt cgtgcgcaac gccttcacgc gctccgacgc gatcatcaag
     7501 acgccctcga acgacccgct caccgccacg gcgatcgcgc gcaccatggt cgagatggac
     7561 cgggaccacc cgctcacccg gcacatgagc gtggtctact ggaagggcgg cgacgagagc
     7621 gtggagaggg agatatacga cccgcgcggc gtcgagaaga tcgtggcctg gggcggcttt
     7681 gccagcatca agcacgtcac gcgctacctg cagccgggcc tcgacctgat cacgctcgat
     7741 cccaagcaca gctcgacgat catcggccgc gaagccttcg ccgacgacgt gacgctggaa
     7801 tcggtggcgc gacgcctggc gctcgacatc ggcgcgatga accaggaagg ctgcgtcaac
     7861 gcgcgcgtgg tgtacgtgca gtcgggcacc gacgccgagg ggctggcgcg cgccaaccgc
     7921 ctgggcgagc tcgccatcgc ggcgctgcag cagttgcccg agttcctgag caccccgcac
     7981 aaggccttcg acgccgcgct gaaggaggag atcgacggcc tgcgctatgc cggcgacgaa
     8041 tacgtggtgt acggcggccg gaagaacgag ggcgcgatca tcgtgtcgca gaatgacgcg
     8101 ccggtggact tcgcgcgcca actcgcctgc cgggtgggca acatcgtgcc gctcgacgac
     8161 atcgacacgg cggtgcgctc ggtcaacgcc tacacgcaga ccatcggcat cttccccgag
     8221 acactgaaga cgcagctgcg cgatcgcctg tcgtaccagg gcgcgcagcg cctggtgtcg
     8281 ctcggcgccg cggcgctgtt cgaggacgcc gcgaccccgc aggacggcat cgagccggtg
     8341 cgccgcatgt gcaagtggat cgtggaacag acatccgatc ccgtgatgct cgacaatctg
     8401 gcgcgcggga caatgtagcc cggcattcat gccgacacga tttgtcgggc tgaagcccga
     8461 cctacggaga ccatgacatg agcgaactgt tccagaacct gctgcgcgac ccgcagagct
     8521 acatcgacgg cgactacctc gcaggacacg cgttcacgcg cgagcagatc gaggccgtgc
     8581 acctcgccgg tctgcgcaag cgcttcaatg aactgcgcgg gcaggtgccg gtgctggacc
     8641 gtttggccac cgagcagggc atcgacgcta tcgactcgct ggaggacgcg gtgccgctgc
     8701 tgtttccgca cacggtctac aagtcctacc cgctgtcaat cctggagcgc gggcagttcg
     8761 accgtctcac gcgctggctc gacgggctca cgacgatcga cctgggcaag atcgacacgg
     8821 ccggcgtcga cagcatcgac ggctggatca agcgcctcga cgagcagacc gacatgcgct
     8881 tgctgcacac cttcggcacc accggcaagc tctcgttcat gccgcgcatg cgcgagcact
     8941 ggatccagtc gaccacgctg acggcccacg cgatccgcga ctggaacggc catggcaccg
     9001 ggcccgacct gatgagcgcg cgcctgccga tggtgcagcc gggctaccgc tacggcgccg
     9061 gtgcgaccta ccgcggcgtc gaggcgatgg tcaagctgtg ggcaggcggt gacgacaacg
     9121 cgctgttcct ctaccccgat gattacttca gcgccgatat cgcctcgctg gccggacgcc
     9181 tgcgtgccgc cgaggcgcgc ggcgagcagg gcaggctgga actctcgccc ggcctgctgg
     9241 cgcggcgcga ggagttcgct catcgcgagc agcagcgccc ggggcgcatg aaggaattcc
     9301 tgcagaccgc gatccggcgc ttcggcggcc gcgacatctg cctgttcgcg gtgtggccca
     9361 tcctgttcga atgggccgag cagggactgg ccgagggcat gcgtggtctc ttcggcacgg
     9421 gcagcctgct gatgagcggc ggcggcagca agggccgcgt gctacccgag gactaccgcg
     9481 aacagatctt cgatttcctc ggctaccgcc gcaccttcga gatctacagc atgagcgaac
     9541 tgatgccggg caacccgcgt tgcgaggccg gcaactacca cgtgccgccg gtgctggtac
     9601 ccttcgtgct cgacccgcaa accgggaaga tgctgccgcg cgccggcacg cagaccgggc
     9661 gcttcgcctg tttcgacatg ctgcccgaca cgtactgggg cgggctggta acgggcgacg
     9721 aggtcacgat ggccggctgg gacgaaccct gtgactgcgg acgcaggagc ccctacctgc
     9781 acggcacgat ccgccgtttc agcgagatcc agggcggcga cgacaagatc aactgcgccg
     9841 gcgcaccgga agcgcacgac cgcgcgatgg atttcctgct gcacgcggcg ggctgatcac
     9901 gttccagcgc ggcatggcgc gggccgggcc catgccgcgc gcggccgccg cctggcacgt
     9961 ccctgtgcgg cggctacgcg tgttgccgaa atcgctcccg gcgatttcgg catccacatg
    10021 tccgctcgcg gcacggaccc ggcccgcgcc tcacgttggc gaagcgtgcc gcgcgcgata
    10081 cgcgtgacgc gccggaccta cgagctgcag gagagcatcg agcagccggc gcggtcgcgt
    10141 gcccagtccg ggcggcgcac ggcgaaggcc tcgcggcccg gctgatcctc gtacggccgg
    10201 cgtaacacct ccagcagttc gtggatgccc gcgtaatcgc cctgctccgc gcggtcgatg
    10261 gcctgctgcg cgaggtagtt gcgcagcaca tagcgcgggt tcgcgcgccg catgcgctcg
    10321 cgccgatccg ccgccggcag cggatcggcg cccacccgcg cgccgtagcg ggccagccac
    10381 gcgaggaacg agccctcggc accccgccgc ttcgcagcgt cgtagaaggc ctcctcgaac
    10441 ggcgccagcg aaggcgccgc cgggtccacc ccgtccagcg cgcggaagaa gatggtcatg
    10501 tcgacctcgg cggactgcaa cagcccgcgc aggtcctcca tcagcgtcac gtcctcgtcg
    10561 cggcaatccg ccagcccgag cttcgcggcg atgttgccgc gctcggcagc gtgccaggcc
    10621 gcaacgtagt gctgcagtcc ctcctgcaat gcctcgacgc cgccgaacgc gggtgccagc
    10681 gcactggcca gccgcccgag gttccagtag gccacctgcg gctgctgccc gaagcggtag
    10741 cgcctcgtcc cggcgtcggt ggtgttcggt gtccagttcg gatcgaagtc gtcgatccag
    10801 ccgtagggac cgtagtcgat cgtgagcccg agaatcgaca ggttgtcggt gttcatcacg
    10861 ccgtgcacga agcccacgcg catccattcc gccatcaggc gcgcggtgcg ttcgcagacc
    10921 tcgcggaacc acgcggcgtg cagttgctcg cggctgccgc gaaggtgcgg gaagtcgcgc
    10981 gcgatcgtga agtcgatgag ccgctccagc aacgccacgt cgttgcgcga ggccggcagc
    11041 tcgaagttgc cgaagcggat gaacgagggc gccacgcgac agacgatcgc accggcctcg
    11101 gcgcgcggat ggccgtcgta gaacatgtcg cgcaccaccg gctcgccggt gcccaccagg
    11161 cacagcgcgc gcgtggtcgg cacgccgagg tggtgcatcg cctcgctgca caggaactcg
    11221 cggatagagg agcgcagtac ggcgcggccg tcggcgttgc gcgaatacgg cgtgggaccg
    11281 gcgcccttga gctgcagctc ccagcgctcg ccgctcgcgt tcaccgtctc cccaagcgag
    11341 atcgcacgcc cgtcgcccag ctgcccggcc cagttgccga actggtggcc accgtagttg
    11401 gccgcgtagg gtgccatgcc ttccagcagc gcgttgccgc cgaacacgcg cgcgaacagc
    11461 tcgctcgcga tgtgctcctc gccgatcccg agcagttcgg ccacctcgcg cgaatgcgcg
    11521 atcagcgccg gcgcggcgac cggcgtcgga tgcacgcgcg agtacagcgc gccgtgcacc
    11581 tggcgcaggc gcgggccgtg ctcgggatcg ccgggcagtt cgttgacgaa gcggttgtcg
    11641 aagcggaggt tgtccatgag gtgaagatat catgagcgga cggcatcgat gcgggggcgg
    11701 tgccgtcagg aatgccgctg tcgccatgaa tggcgaccca cggtcgggca ccggatctac
    11761 ccgattcggg taatacatat gaatgattcg cacgtcatga tttggcccta ctttgagcat
    11821 gcattttcaa cccgggcagg gagcagacca gatgacagac aagaagacat tgcgtgacat
    11881 ccgcgtggtg gtgatgggag ccggcatggc tggcatcctg agcggcatca agctgctcca
    11941 ggccggcatc acgaactgcg cgatctacga gaaagccgac cgcatcggcg gcacctggcg
    12001 cgagaacacc tatccgggcc tgacctgcga cgtgccctcg cacgcctaca cctactcctt
    12061 cgagcccaac cccgactgga gccacatgct gccgccgggc gccgagatcc aggagtattt
    12121 cgagcggacc acgcgcaagt acggcgtcga caagatcatc cgcttcaacg aggagatcgt
    12181 gcgttgcgag ttccgcgacg gtcgctggca tctcgaaacg aagaacggca cccgggatca
    12241 agctgacatc gtgatcgcgg ccaccggcgt actgcaccac ctgaactacc cggacattcc
    12301 ggggctcgcg agcttcgagg gcgcctgctt ccacaccgcg cgctgggacc acagcgtgcc
    12361 gctcgacggg cgccgcgtcg gcgtcatcgg caacggctcc acgggcgtgc agatcatctc
    12421 ggcgctggcg ccacgcgccg gcaagctgca ccatttccag cgcaccgcgc agtggatcat
    12481 gccggtcgag aacccggcgt tcaccgatga gcaacgcgcg gcgtttcgcg ccgatcccgc
    12541 actgctgcac tacatgcaga accagcccga ctacctggcc aacgtcgagc gcttcaacaa
    12601 ttcactggtc aacgccgact ccgagggcat cgccgagatc gaggccacct gcctcgcgaa
    12661 cctcgagaac accgtgcgcg accccgtgct gcgcgaaaag ctgcgccccg actaccgcgc
    12721 cgcgtgcaag cgcctgatct actcgcccga ctactacgag gcgatccagc accccaatgc
    12781 cgagctggtg accgacggca tcgaatgcat cgagcccgcc ggcgtgcgct tgaagaacgg
    12841 ccgccttgtc gagctcgacg tgctggcgct ggccaccggc ttcaatgccg gcatgttcat
    12901 gcgcccgatg aatgtggtcg ggcgcgacgg cgtgcagctg aacgacgtct gggcggaccg
    12961 ccccaaggcc tacatggcga tcgccatgcc cgacttcccg aacttcttca tgctgaacgg
    13021 ccccaacggg ccggtgggca acttctcgct gatcgacatc gccgagcagc agctgcacta
    13081 catctggacg ttcatcgaga agctgcgcgc gggcgagtgc cgcgaagtgg caccgacgcc
    13141 tgaagccatg caggccttcg agcgcgaccg cctcgaggcg gcgaagcgca ccgtgttcgg
    13201 ctccggctgc aagagctggt acctggacgc cgagggcata cccgccacct ggccgtggac
    13261 gcgcacgcgt ttttgcgagg agatgaaggc gccgcggctg gaggcttacg ccttcgccag
    13321 ctgaaccggc gtccgcatgc cggatgtcgg acccgcggcc cgacatccgg caactgccgc
    13381 accagctgct gcacttgcca gcgcacggcg cgataatcag cgtcccgctg gaccaggaag
    13441 caaccgacca tgagacgact cgtcaccgga atcgacgccg acggcaaatc cgtgttcgtg
    13501 cacgacggcc cctgcccgca gcccgtcagc ggcgccggat tcgagctggg cgcgctgtgg
    13561 gccagcacgc cggacacgcg gctcggcgaa tccggcacgg acgccgcgat tggcaagacc
    13621 tcgaccgtgc cgccgcccgg cgccacggtg ttcacctacg tcgtgatccc gcccgacgcc
    13681 gaagcggcac ggggccaggc cgataccgtc tccgcctcgg ccgatttcaa cgtcgaggac
    13741 gacggccgca tgcaccgcac cgacaccatc gactacggcg tgctgctcgc cggtgaactg
    13801 tggctcgagc tcgatgacgg ccgccagcag ctgctgaagg ccggagacag cttcgtgcag
    13861 aacggcacgc gccacgcctg gcacaaccgt ggcagcaaac ccgcgatcat gtgcgcggtg
    13921 atgatcggca tggcgcggga agcctgaggc aacgccccgc aggtcggcat tcctgccgac
    13981 tcgcgccttg tcgggctgaa gcccgaccta caagccctac cttcgatgcc tgcgatagcg
    14041 gcgtcttgtc gtcagaagcg agccgtcatt tccccggccg gcgttgacgc gccggtttgg
    14101 gcctggtggc cggctgcctg agcttgcctt gcgcctgttc cagccacagc agcgaatcca
    14161 cctgcgacag gaatcgccgc agatagtcgg gcgacagctc gcgcatcagc gtcagggacc
    14221 gcagcaccag catgtgcgaa ttgagcggac cggcgttctc gggccctcgg ccgacggcct
    14281 gttccagcgc gtcctcggca cagatcctgg accaggcctc ccggaaacgg tgcgcgctct
    14341 tcagctcggg cgaagcatg
//
LOCUS       DAORUQ010000077        14310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4087_length_14310_cov_6.301298, whole genome shotgun sequence.
ACCESSION   DAORUQ010000077 DAORUQ010000000
VERSION     DAORUQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_14310_cov_6.301298"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1143)
                     /locus_tag="PLW13_08420"
     CDS             complement(175..1143)
                     /locus_tag="PLW13_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70437.1"
                     /translation="MANEDLFVALTECHFMNPQTMSEIAYYPAMKRWNDSVDGVLRAW
                     AGRDLASEWPHPLAEALIGYMDEARVDVCFCLREPMMDISGGSASMSTNGFMLQQIEP
                     YPGRMYLEANCGPVIRRGLEHAIWELEYLVKERGAKLCKMYQPEDAGPINDRRLWPFY
                     ARAEELGVPLTVHTGNSYVVPQPGSHCDVRQLDDVMLAFPDLKVIAYHAGWPDTEALI
                     GLCGKHVNLYMSLSGIIGWYQRAPYRGYHAIGTAMQWMPADKIVLGLDLPFDDTRRVV
                     DYIRDLEMPEELQKNWGYAQPTREDKAKILGLNLARLTGIDPVKRS"
     gene            1347..2303
                     /locus_tag="PLW13_08425"
     CDS             1347..2303
                     /locus_tag="PLW13_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQY70438.1"
                     /translation="MSVVLCSWGDAIEGGTEEALSLAGRLSRDRGAGLDWLLIGNVTD
                     DVSGMAARHGVAHIDRASASAPGPDTLVAMIVAYCSQHRPTVILFNQTLAARLVAPRV
                     AARLGVPVVMNGFYIESQGSELEVRTTAYGGDTHAVYRLPAPVNVVSIVTTTLRVEAA
                     PQPSVPVTREVALPAGGVTERWTVISAARSEGPRLEDADVIVSGGRGLGSAANYRLVR
                     ELAEQIGGMYGASRAIVDDGWVDSSRQVGLTGKITRPGLYVAVGISGASQHMAGCSAA
                     KTIVAINKDRDASIFRYARYGIAGDCLDVMPELIKAAAAGNH"
     gene            2316..2756
                     /locus_tag="PLW13_08430"
     CDS             2316..2756
                     /locus_tag="PLW13_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY70439.1"
                     /translation="MSDEILPCVPGLFDQSGGAARLHGSRCASCRTPYFPRSAECHNP
                     DCGGSRMEDCSFAGNGVLWSFSVADFPPPPPHRYDKPFVPYAIGVVDLDCGLRVVGQM
                     VDPPSALRVGDTVELVISELYHEDGKAFTSWKFRQLAATAAGSE"
     gene            2758..3951
                     /locus_tag="PLW13_08435"
     CDS             2758..3951
                     /locus_tag="PLW13_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70440.1"
                     /translation="MQEVAVLGVGLHRFGKTGDDIVGNKSVTALCRHAIDAALSDAGV
                     PWKQIQAVCAASSRFSGGKGWGLNGNDVVEELGSTGIPVYNMSAGCAAGGNAFNVGYS
                     MVAGGVYDMVLVVGGEVMPKGMIQTSGVEEATDPEFLRQRCVGMPGPSFWATLARRRM
                     HDFGTTEEQFAKVAVKARKCSVHNPYARFQKEIGLDEVLASPYVSNPLRLFEICPVSN
                     GAAAVVICSAAKARQFTSRPVLVASSTVATMHFSDALPRGLAGPVPGGPSFHTESLAA
                     VKRAFEMAGIGPQDVSLTELQDNTCYYEFAYPEEWGLCKPGEAERLLESGQTAPNGRM
                     PINPSGGFVSFGEATTAMGVFQIVELTWQLRGQSGGRQVPDARVGLAQTNGLGGNATA
                     AILKK"
     gene            3998..5971
                     /locus_tag="PLW13_08440"
     CDS             3998..5971
                     /locus_tag="PLW13_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQY70441.1"
                     /translation="MPEISAPSYFLLGLLLVAALASLFRGLRAHRDRIARGKAFFGPR
                     IDRASVLARINWRAFISRGVFTSRLRQRRWSGIAHGLLFFGALALICGHALFALSFVG
                     VPVYAGWFGYLVMELGREIAGIMVFLGVLFFLLRRFFPPQRLVAGGKRPGFERMEALI
                     LLTVVAGYSSESFRLALQPGGGGEFLGTAVAAFIRQHLDPDATLLGFKLLWWLHGLLG
                     ASFIALIAHTPMSHMLLGPTNSALASRRSGINLAPLDFDAEQDAAGNPVVFGAARLAD
                     LTQKNLLDVSACLWCGRCHEVCPAAQTGKDLSPKKVMATCAEYLAAGRFDDNSLIEVL
                     GRDAIFNCTTCAACVEVCPVSNNPAEVILEFRRHHVMELSDMPDTMAAANRNLESRGH
                     PFVGTAANPDDWRSGLQVPIFEPGSTEYLLWIGCSVRYEPRAQETARAMVRILEAAGL
                     SYGILGESRCTGDPAKMMGNEMQFVETAQENIEEFREIGVQKVITLCAHCFNSFDRYY
                     PELGADWKTIPHSVLIDELIADGRLQIAHKGEETITFHDPCYLARHNGIVDETRRVLS
                     AVGQLIEMPRTREQSFCCGAGGGNYWGGQGGTARIGDVRIQEALDTGADKVATACSFC
                     TLMLTASAGKHSETRKVFDVAELVAERIRIVEG"
     gene            complement(6021..6665)
                     /locus_tag="PLW13_08445"
     CDS             complement(6021..6665)
                     /locus_tag="PLW13_08445"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY70442.1"
                     /translation="MAIEIRETFQVSAPIDQVWNFMKSPENLAGCMPGASLARIIDDR
                     SFVGTVKLKVGAITASYDGTMTFIKLDDSARELTLLAEAKEKGGGTVSGTISANLLAL
                     PDGSTEVRCESSTDLTGRIVQVGRGMIEGVSKQVIQKFVTNVKSSLESVPAAAPAGED
                     ASAGVATPAPGAAPSEPVARPPIAKEESINVLAVVWNVIWAAIRGFFRRLFGRA"
     gene            6872..8563
                     /locus_tag="PLW13_08450"
     CDS             6872..8563
                     /locus_tag="PLW13_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY70443.1"
                     /translation="MSGHDWDHTTDVLVIGSGAGALTAALRAHDNGAQTLVIEKTALY
                     GGSSAMSGGVLWLPSSPMIARAGGSDSPEEAKRYMKSVIGEPSQNARIDAFVDTIPRF
                     IEYLHARTRVYLESLVGFPDMYPDNPDFKMHRCHEPLPFNGGSLGDELQFLRPQHPQT
                     TLFGVIGWTASESLVLQARGKGWLWVAFSMVARYVLDIPGRLRGMRDRRQVLGGALVG
                     SLRRSMIERGMPLWLGTAAQELVTEDGRVTGVTALRDGKWVRIRARKGVIVASGGFEK
                     NNAMRRRHLPQPTDAAWTAGSPGNTGEMIEASASVGAATRFMDAAWWGPTFLIPGEAQ
                     ARMLIIEKNLPGCLIVNRVGERFVNESSSYTKVTKGILAANAPGRESVPAALVFDSTY
                     RKHYPFGPMLPSSFQPDFAVPRSAWGGIMKADTIEELARQLDVDPAGLAATVRKFNGY
                     AASGKDPDFQRGDATYDRYYGDHSVGPNPCLGPISKPPFYGAKVYPGELGTKGGIDTD
                     ENARVLDQGGNVIPGLYAIGNCSASVMGQTYPASGSTLAPAMVFGFRAADDATGQ"
     gene            8628..9146
                     /locus_tag="PLW13_08455"
     CDS             8628..9146
                     /locus_tag="PLW13_08455"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HQY70444.1"
                     /translation="MASTDRESMEKPDFMSDEDWEQIKAQTATLDRIRGDARADVARY
                     LANPEGWATGAGSPSGLPTLLLTCIGRKSGEQHTTPLVFMQDGEDMVVVGSLAGYDTH
                     PAWYLNVSANPQCWVQLDRRKMTATARDATDAERKELWPRLTAIFPAWGHFQKQTDRP
                     FAIVILSPTGPA"
     gene            9176..11173
                     /locus_tag="PLW13_08460"
     CDS             9176..11173
                     /locus_tag="PLW13_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70445.1"
                     /translation="MSNVNFEHLFSPLQVGPMRVPNRICETTNTINSSMTPGLIDEHF
                     IAHHAAKARGGTGWIGSETWLLDAPFPPETPDEIGLPVGCAGHQAAYQNPAFAESMAR
                     FCAEVHQSGSVVVVQLTHLNAVWAPSPVPVIGAQDYTPHVMGEEEIEFCLDTYASAAA
                     VARAAGADAIEIHCAHETLGYSFLSPVTNRRADKWGGGPEQRARFVIEALKRVRACVG
                     ESMALGIRINGQESRHGGYDNLEMREMLYCIGETGLLDFVDIDTGHCWGAPSYVPSSY
                     HPHAEFREFGKAARADLALMERKIAVLFTGRINDPVLAEELIKEGFCDLVGMVRAGIA
                     DPEFANKAREGRLGEIRRCIGCTRCIDEASEPTTMPYTPTCSINPVIGNELRWQQQYR
                     PAQEPKRVVVVGGGIAGCEAARVAAMRGHRVILLEQGKRLGGQLLIAARAPGRDSFED
                     QAYFEENEMARLGVDVRLQSVADVAAIKALAPEAVVIATGSVPRVPDDVPGIDLPHVA
                     QGWDVMRGKVSTGERVAVISQEDYYQTPAVAEFLAARGKQVEVFHKSVHLGTEIARYS
                     IGMVLSRMEQCGIQIHPNLVLKELWSDGLEFVSSWGGKTYRREGFDSVVLVYGSTPQH
                     GLYDQLKAEGCIPQLYLAGSAWLPRRLAEATRHGAGIGLVI"
     gene            complement(11214..12116)
                     /locus_tag="PLW13_08465"
     CDS             complement(11214..12116)
                     /locus_tag="PLW13_08465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70446.1"
                     /translation="MRFGFHLFMVDPDQHLDIARTADDCGWDSIQVADSPFFPETISV
                     PYPYTPDGTRFWPLDMPVLDPWVAITAMAVVTRRIRFLPSVLRLAIRQPLLEAKSLCS
                     VAAIANDRVALGVGLAWMPEEFKWLGVDMKTRGARQDEAIQVIRLLMRGGMQEFHGKY
                     FDFDPLIMAPVPKKRIPIYVGGTTMPALRRAARYGDGWVSVIHDMDAVADMLAQLERF
                     RREFGRENEPFDIMMHCPDAQTVDDIRRLEDIGVTDLQVTPWSVPGILAEMGVGAIMQ
                     QQPPLDVKLEAIKRYAGQVIARFA"
     gene            complement(12210..13418)
                     /locus_tag="PLW13_08470"
     CDS             complement(12210..13418)
                     /locus_tag="PLW13_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70447.1"
                     /translation="MINPEFSLPVEVPLRFLRTAPWWLLVAAVLFAGHATEVLASRYH
                     PVTLAIVHLMMLGFAGNIMLGALLQISAVLAGARAQRPVLAGWLVWGSLQTGTALLAA
                     GLWLMQPLLLQAAGAVLGVMLLAFTGWLFTRLWRSPAVDASTAGLRWSVAGLGLTVAC
                     GILLLLVLTRGVALALPEVLRLHVLFGSMGWVFPLLASVALTVVPMFLVTPPWPQRLA
                     RLLVPLNALMLLLLAVWPVLLPLLVLPVLLFVLQLLRSLKASKRGADPARWLWAWGGV
                     NLLLVCALAPWLDALPRLNPVLLGGQFLCGALLPIYTAMLGKIVPFLLWLDYRASVPV
                     GRKLRHMGQLFPERWLRGLGGLALAVGLTWPLALASIWPVLVLLLVYGVALLVVIHRA
                     CRNARAAITG"
     gene            complement(13405..13638)
                     /locus_tag="PLW13_08475"
     CDS             complement(13405..13638)
                     /locus_tag="PLW13_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQY70448.1"
                     /translation="MDDCIDLSGLAAPEPMDRVLDWLDAAAPGACQAFLLPRMPYPLF
                     DHLARRPCDWETEPQPDGRAVVTIRRNASRDQS"
     gene            complement(13661..14071)
                     /locus_tag="PLW13_08480"
     CDS             complement(13661..14071)
                     /locus_tag="PLW13_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQY70449.1"
                     /translation="MGIVTKMQAQHRHCDELLAQAEAAARREDWTACAAASAAFVADT
                     EAHLALEENGLFPAFEKATGMSSGPTQMMRIEHAEVRDLMEGLNEALTARNAADFVGC
                     CETLLILLQQHNMKEENVLYPMCERAVPEFAGQL"
     gene            complement(14141..14296)
                     /locus_tag="PLW13_08485"
     CDS             complement(14141..14296)
                     /locus_tag="PLW13_08485"
                     /inference="COORDINATES: protein motif:HMM:NF023158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HQY70450.1"
                     /translation="MRFNGKERTNVEEYCVSEGWIRVAAGRAVDRKGNAVTLKLKGTV
                     EPYFKDV"
BASE COUNT         2466 a         4848 c         4708 g         2288 t
ORIGIN      
        1 acggcgctgc gcaaccgcgt gcccgctttc aacgtcgcca tatgatcacc tccgagcatc
       61 ggttcgagta tttcgccaat tccgtctcgc gcgggtccag cccggcacca cccgcccgga
      121 ctgtcccgac accggcaagg cgaaggccgg gcccgccggg cccgggccgg gttgtcagct
      181 gcgcttcacc ggatcgatgc cggtgagccg ggcgaggttc aggccgagta tcttggcctt
      241 gtcctcgcgc gtgggctggg catagcccca gttcttctgc agctcctcgg gcatctcgag
      301 gtcgcggatg taatccacca cgcgccgggt atcgtcgaag ggcagatcga gaccgagcac
      361 gatcttgtcg gccggcatcc actgcatggc ggtaccgatc gcgtggtagc cgcgataggg
      421 tgcgcgctgg taccacccga tgatccccga gaggctcatg tagagattca cgtgcttgcc
      481 gcacagcccg atcagcgctt ccgtgtcggg ccagccggcg tggtaggcaa tcaccttcag
      541 gtccggaaac gcgagcatga cgtcgtcgag ctggcgcaca tcgcagtgcg agcccggctg
      601 cggcaccacg taggaattgc cggtgtgcac cgtgagcggc acgccgagct cctcggccct
      661 ggcgtagaag ggccacaggc gccggtcgtt gatcggaccg gcgtcctcgg gctggtacat
      721 cttgcagagc ttggcgccgc gctccttgac caggtactcg agctcccaga tcgcgtgctc
      781 cagcccacga cggatcaccg ggccacagtt ggcttccagg tacatccgcc ccgggtaggg
      841 ctcgatctgc tggagcatga agccgttggt ggacatcgac gccgagccgc cgctgatgtc
      901 catcatcggc tcacgcaggc agaagcagac atcgacgcgc gcctcgtcca tgtacccgat
      961 cagcgcctcg gccagcggat gcggccactc gctagcgaga tcacggccgg cccaggcgcg
     1021 cagcaccccg tcgacgctgt cgttccagcg cttcattgcc gggtagtacg caatctccga
     1081 catggtctgc ggattcatga agtggcactc ggtgagcgcg acgaacagat cttcgttggc
     1141 catggtcact ttcctccgat cgtggttttg gcgtgccgcg tcaccagacg ccagccgggc
     1201 acaacataca ccgcgtgcaa cagccgttca ccgccttgtc cggatttgag acgccgtcgt
     1261 ccggatttga tccgctgcgg aaccgcatcc ggtcggagcg ccgattagac tgtgtccatt
     1321 cggcgcaaat gcaggaggaa gcggcgatga gcgtggtact gtgcagctgg ggggatgcga
     1381 tcgagggggg aaccgaggag gccctttcgc tggccgggcg cctgagccgc gatcgcggtg
     1441 ccggactcga ctggctactg atcggcaacg tgaccgacga cgtgagcggg atggcggcgc
     1501 ggcacggcgt tgcccacatc gaccgcgcga gcgcgtccgc gccggggccg gataccttgg
     1561 tggcgatgat cgtggcctac tgctcgcagc accgccccac cgtcatcctg ttcaaccaga
     1621 cgctggcggc ccggctcgtg gcaccgaggg tggccgcgcg tctcggcgtg ccggtggtga
     1681 tgaacggctt ctacatcgaa tcccagggca gcgagctcga ggtgcgcacc accgcctacg
     1741 gcggggatac gcacgcggta taccggctcc cggcacccgt caacgtggtc tccatcgtca
     1801 ccaccacgct gcgcgtcgag gcggcaccac agccgagcgt tccggtcacg cgcgaggtcg
     1861 cgctgcccgc aggcggcgtg acggaacgct ggacggtgat ctcggcggcc cgatccgaag
     1921 gaccgcgcct cgaggatgcg gatgtcatcg tctccggcgg gcgcgggctc ggcagcgccg
     1981 cgaactaccg cctggtgcgc gagctcgccg aacagatcgg cggcatgtac ggcgcctcgc
     2041 gcgccatcgt cgacgacggc tgggtcgatt cctcacgcca ggtcggactc acggggaaga
     2101 tcacgcgccc gggtctctac gtcgccgtcg gcatctccgg ggcgagccag cacatggcgg
     2161 ggtgctccgc cgcaaagacc atcgtcgcga tcaacaagga ccgcgatgca tcgatcttcc
     2221 gttatgcgcg ttacggcatc gcgggcgact gcctggacgt gatgccggaa ctgatcaagg
     2281 ccgctgcagc gggcaatcac tgaaggaggc tgcacatgag cgacgagata ctgccctgtg
     2341 ttcccggact gttcgaccag agcggtggcg cggcgcgact gcacggctcg cgctgtgcga
     2401 gctgccgcac gccctatttc ccgcgatccg ccgaatgcca caatcccgac tgcggcggct
     2461 cgcgcatgga ggattgcagt ttcgcgggca acggcgtgct gtggagcttt tcggtggcgg
     2521 attttccgcc gcctccgccg caccgttacg acaagccctt cgtgccctac gcgataggcg
     2581 tggtcgacct cgactgcggc ctgcgcgtgg tcggccagat ggtcgaccca ccgtcggcgt
     2641 tgcgcgtggg cgatacggtg gaactcgtga tcagcgagct ctaccacgag gacggcaagg
     2701 cgttcacgtc gtggaaattc aggcagctcg ccgcgaccgc ggcagggagt gagtgacatg
     2761 caggaagtag ccgtactggg tgtcggtctg caccgattcg gcaagaccgg cgacgacatc
     2821 gtgggcaaca agtcggtcac ggcgctgtgc cggcacgcaa tcgacgcggc gctgagcgac
     2881 gcgggcgtgc cgtggaagca gatccaggcc gtctgtgccg ccagttcacg cttctcgggc
     2941 ggcaagggct ggggcctcaa cggcaacgac gtggtggagg agctgggctc cacggggatc
     3001 ccggtctaca acatgtcggc aggctgtgcg gcgggcggca acgcattcaa cgtcggctat
     3061 tcgatggtcg ccggcggcgt ctacgacatg gtgctggtgg tcggaggcga ggtgatgccc
     3121 aagggcatga tccagacctc gggcgtcgag gaggcgacgg atccggagtt cctgcgccag
     3181 cgctgtgtcg gcatgcccgg cccctcgttc tgggccacgc tcgcgcgccg gcgcatgcac
     3241 gacttcggca ccacggaaga gcagttcgcg aaggtggcgg tgaaggcgcg caagtgttcc
     3301 gtgcacaacc cctacgcgcg cttccagaag gagatcgggc tggacgaagt actcgcctct
     3361 ccctacgtca gcaatccgct gcgactcttc gagatatgcc cggtgtccaa cggcgccgcg
     3421 gccgtggtga tctgttcggc ggcaaaggcg cgccagttca cctcgcggcc ggtgctcgtc
     3481 gcatccagca cggtggcgac gatgcacttc agcgatgcgc tgccgcgcgg gctcgccggc
     3541 ccggtgcccg gcggccccag cttccacacc gaatcgctcg ccgcggtgaa acgggcgttc
     3601 gagatggccg gcatcggtcc gcaggatgtg agcctcacgg agttgcagga caacacctgc
     3661 tattacgagt tcgcttaccc cgaggaatgg ggcctgtgca agcccggcga agccgaacgc
     3721 ctgctggagt ccggacagac cgcgccgaac ggtcgcatgc cgatcaaccc ttcgggcggc
     3781 ttcgtgtcct tcggcgaggc cacgacggca atgggcgtgt tccagatcgt cgaactgacc
     3841 tggcagctgc gcggccagtc gggcgggcgc caggtacccg atgcgcgcgt cgggctggca
     3901 cagaccaacg gactgggcgg caacgcaacg gcggcgatcc tgaagaagta gccgcggcgg
     3961 agccggtata ctcggcaaaa atctaggcga gcacgcgatg cctgaaatat ccgcccccag
     4021 ctatttcctc ctcggcctgc tcctggtcgc ggcgctcgcc agcctcttcc ggggcctgcg
     4081 cgcgcaccgc gatcgcattg cgcgcggcaa ggcattcttc ggtccgcgca tcgatcgggc
     4141 cagcgtgctc gccaggatca actggcgcgc tttcatctcg cgcggcgtgt tcacttcgcg
     4201 cctgcgccag cggcgctggt ccggcatcgc gcacgggctg ctgttcttcg gcgcgctggc
     4261 gctcatatgc ggccacgcgc tcttcgcgct gagctttgtc ggcgtgccgg tgtacgccgg
     4321 atggttcggc tacctggtga tggaactggg acgcgagatc gcggggatca tggtgttcct
     4381 cggcgtgctg ttcttcctgc tgcgtcgctt cttcccgccg caacggctgg tggcgggcgg
     4441 caagcggccc ggcttcgagc gcatggaggc gctgatactg ctcaccgtcg tcgcgggata
     4501 ctcctccgag agttttcgcc tcgcgctgca acccggcggt ggcggcgaat tcctgggcac
     4561 cgccgttgcc gcgttcatcc ggcagcacct cgaccccgac gccacgctgc tcggcttcaa
     4621 gctgctgtgg tggctgcatg gcctgctcgg cgcctcgttc atcgcgctta tcgcccacac
     4681 cccgatgtcg cacatgctgc tcggtccgac gaactccgcg ctggcgagcc gccgatccgg
     4741 gatcaacctc gcgccgctcg acttcgatgc cgagcaggac gccgccggca acccggtggt
     4801 tttcggcgcc gcgaggctcg ccgacctgac gcagaaaaac ctgctcgacg tgtcggcctg
     4861 cctgtggtgc gggcgctgcc acgaagtgtg tcccgccgcc cagaccggca aggacctctc
     4921 cccgaaaaaa gtcatggcga cctgcgccga atacctggcg gcgggtcgct tcgacgacaa
     4981 ctcgttgatc gaggtgctcg gtcgcgatgc gatcttcaac tgcaccacct gcgccgcctg
     5041 cgtcgaggtc tgcccggtca gcaacaatcc cgcggaggtg attctcgagt tccgccgcca
     5101 tcacgtgatg gaactgtcgg acatgcccga caccatggcc gcggcgaacc gcaacctgga
     5161 atcgcgcgga caccccttcg tgggtaccgc ggcgaacccc gatgactggc gcagcggact
     5221 gcaagtgccg atattcgagc ccggcagcac ggaatacctg ctatggatcg gctgttcggt
     5281 gcgctacgag ccgcgcgccc aggaaacggc gcgcgcgatg gtgcgcatcc tcgaggccgc
     5341 gggactgtcc tacgggatcc tcggcgagtc gcgctgcacc ggtgatcccg cgaagatgat
     5401 gggcaacgag atgcagttcg tcgagaccgc ccaggagaac atcgaggagt tccgggagat
     5461 cggggtgcag aaggtcatca ccctgtgcgc gcactgcttc aacagtttcg accgctacta
     5521 ccccgagctc ggcgccgact ggaaaacgat cccgcactcg gtgctgatcg acgagctgat
     5581 cgccgacggc cgcctgcaga tcgcgcacaa gggcgaggag acgatcacct tccacgatcc
     5641 atgctacctg gcgcggcaca acggcatcgt cgacgagaca cgccgcgtgc tctccgcggt
     5701 gggccagctc atcgagatgc cccgcacccg tgagcagagc ttctgttgcg gggccggggg
     5761 cggcaattac tggggtggcc agggcggaac cgcgcgcatc ggcgatgtgc gcatccagga
     5821 agcgctggac acgggcgccg acaaggtcgc gaccgcgtgc tccttctgca cgctgatgct
     5881 caccgcgagc gcgggaaagc acagtgaaac gcgcaaggta ttcgacgtcg ccgaactggt
     5941 cgcggagcgc atccgcatcg tcgagggctg aacgggcacc ggtcgggctg ccatggcggc
     6001 ccgcccggtg ccgggcgcgc tcaggcgcgc ccgaacagcc gacggaagaa gccgcggatc
     6061 gcggcccaga tcacgttcca caccacggcg agcacgttga tcgattcctc cttcgcgatc
     6121 gggggtcgtg ccacgggctc tgatggcgcg gcacccggag ccggcgtcgc gacgcccgcg
     6181 gacgcgtcct cccccgccgg tgccgcggcc ggcaccgatt cgagcgaact cttcacgttg
     6241 gtgacgaact tctggatcac ctgtttcgag acgccctcga tcatcccgcg ccccacctgc
     6301 acgatgcgcc ccgtgagatc ggtgctggac tcgcagcgca cctcggtgct gccgtccggc
     6361 agcgcgagca ggttcgccga tatggttccg ctgacggtgc caccgccctt ttccttggcc
     6421 tcggccagca gggtgagctc gcgcgcgctg tcatcgagct tgatgaaggt catcgtgccg
     6481 tcgtagctcg ccgtgatggc acccaccttc agcttgacgg taccgacgaa gctcctgtcg
     6541 tcgattatcc tcgccagcga ggcgcccggc atgcatcccg cgagattctc ggggctcttc
     6601 atgaagttcc acacctggtc gatcggagcg gaaacctgga acgtttcgcg gatctcaata
     6661 gccatgtttc atccccttca ggtgaattca ccctcgacaa acgcaaccgc actgacacgc
     6721 atggtagccg ccaaggtagc tgaattcccc gcgtcgcgaa atccgaatcg gccgaatcgg
     6781 tatcagaacg ggacaacccg cacgcacgca cgcgcagcga tccccgtact gtgatttact
     6841 cggatccatg ccattcgcag ggagtccgga catgagcggc cacgactggg atcacaccac
     6901 cgacgtactc gtcatcggtt cgggagccgg tgcgctgacg gctgcgctgc gcgcccacga
     6961 caacggcgcg cagactctcg tcatcgagaa gaccgcgctc tacggcggat cgtccgccat
     7021 gtccggcggg gtgctgtggc tgccctcgag cccgatgatc gcgcgcgccg gcggcagcga
     7081 ctcgcccgag gaagccaagc gttacatgaa gagcgtgatc ggggaaccct cccagaacgc
     7141 gcgcatcgac gccttcgtcg acacgattcc gcgattcatc gagtacctgc acgcgaggac
     7201 ccgcgtctac ctcgaatcgc tggtcgggtt ccccgacatg tacccggaca atccggattt
     7261 caagatgcac cgttgccacg agcccctgcc gttcaacggc ggcagcctgg gcgacgaact
     7321 gcagttcctg cgtccccagc acccgcagac gacgctgttc ggggtcatcg gctggaccgc
     7381 gagcgagtcg ctggtgctgc aggcgcgcgg caagggctgg ctatgggtgg cgttcagcat
     7441 ggtcgcgcgc tatgtgctcg acattcccgg ccggctgcgc ggcatgcgcg atcgccgcca
     7501 ggtgctcggc ggcgcactgg tgggcagcct gcgccgctcc atgatcgagc ggggcatgcc
     7561 gctctggctc ggcacggctg cgcaggagct ggtgaccgag gacgggcgcg tgaccggcgt
     7621 cacggcgctg cgcgatggca agtgggtgcg catacgcgcg cgcaagggcg tgatcgtcgc
     7681 cagcggcggc ttcgagaaga acaatgcgat gcgacggcgg cacctgccgc agcccacgga
     7741 cgcggcgtgg accgccggct ccccgggcaa cacgggggag atgatcgagg cgagcgcatc
     7801 ggtcggtgcg gccacgcgct tcatggacgc cgcgtggtgg ggtccgacat tcctgattcc
     7861 gggcgaagcg caggcacgca tgctgatcat cgagaagaac ctgcccggct gcctgatcgt
     7921 caaccgcgtc ggcgaacgtt tcgtgaacga gagctcgtcg tacaccaagg tcacgaaggg
     7981 gatactcgcg gcgaacgcgc ccggccgcga gagcgtgccg gcggcgctgg tgttcgattc
     8041 gacctaccgc aagcactatc ccttcggacc catgctgccg agcagcttcc agccggactt
     8101 cgcggtaccg cgcagcgcct ggggcggcat catgaaggcc gacaccatag aggagctcgc
     8161 gcgacagctc gacgtcgatc ccgccgggct cgccgcgaca gtgcgcaaat tcaatggcta
     8221 cgccgccagc ggcaaggacc cggatttcca gcgcggcgac gcgacctacg accgctacta
     8281 cggcgaccac tcggtgggac ccaacccctg tctgggcccg atctcgaaac cgcccttcta
     8341 cggcgccaag gtctatcccg gcgaactcgg caccaagggc ggcatcgaca ccgacgagaa
     8401 tgcgcgcgtg ctggaccagg gcggcaacgt gatccccggg ctctatgcga taggcaattg
     8461 cagcgcctcg gtcatgggcc agacctaccc ggcctcgggc tcgacgctgg cgccggcgat
     8521 ggtattcggc ttccgcgcgg ctgacgacgc gacgggccag tgacaccgca ggcacgcaca
     8581 gacccatgac cgtccacgca atgatcacct gactcgggag gctcatcatg gcaagcacag
     8641 atcgtgaaag catggagaag ccggacttca tgtccgatga ggactgggag cagatcaagg
     8701 cccagactgc aacgctggat cgcatcaggg gtgatgccag ggcggacgtg gcgcgctacc
     8761 tggccaatcc cgagggctgg gccacgggag ccggttcccc ctcggggctg ccgaccctgt
     8821 tgctgacctg tatcggtcgc aagtcgggcg agcagcacac caccccgctg gtcttcatgc
     8881 aggacggcga ggacatggtg gtcgtcgggt cgctggctgg atacgacacg catcctgcct
     8941 ggtacctcaa tgtcagcgcg aacccgcagt gctgggtcca gctcgatcgc aggaagatga
     9001 cggccaccgc gcgcgacgca acggatgcgg agcgcaagga gttgtggccc agactgacgg
     9061 cgatctttcc ggcctgggga catttccaga aacagaccga ccgccccttt gcgatcgtga
     9121 tcctctcgcc gaccggcccc gcgtgatcgc cgggcattca ggagcgccac gcgacatgag
     9181 caacgttaat ttcgagcacc tgttctcgcc attgcaggtc gggcccatgc gcgtgcccaa
     9241 ccgcatatgc gagaccacca ataccatcaa ttcgtcgatg acgccgggcc tgatcgacga
     9301 gcacttcatc gcgcaccatg ccgcgaaggc gcgcggcggt accggctgga tcggcagcga
     9361 gacctggctg ctggatgcgc cctttccccc ggagacgccg gatgaaatcg ggctccccgt
     9421 gggctgcgcc gggcaccagg cggcgtacca gaatccggcc tttgccgaga gcatggcgcg
     9481 gttctgcgcc gaggtgcacc agagcggctc cgtcgtggtg gtccagctga cccacctcaa
     9541 tgccgtgtgg gccccctcgc cggtgcccgt gatcggcgcg caggactata cgccccacgt
     9601 gatgggcgag gaggaaatcg agttctgcct cgacacctat gccagcgccg cggcggtcgc
     9661 gcgcgcggcg ggcgccgacg ccatcgagat ccattgcgcc cacgagaccc tcggctacag
     9721 cttcctgtcg ccggtgacga atcggcgcgc cgacaaatgg ggcggcggtc cggagcagcg
     9781 cgcccgcttc gtgatcgaag ccctgaagcg cgtgcgcgca tgtgtcgggg agtcgatggc
     9841 gctcggcata cgcatcaacg gccaggaatc ccgccacggc ggctatgaca acctggaaat
     9901 gcgcgagatg ctctattgca tcggcgagac cggcctgctc gatttcgtgg acatcgatac
     9961 cggtcactgc tggggagcgc cgtcctatgt gccgtcttcg taccatccgc acgccgagtt
    10021 tcgcgagttc ggcaaggcgg cgcgcgccga cctcgctctc atggagcgca agatcgccgt
    10081 gctgttcacc ggccgcatca atgatcccgt cctggccgag gaactcatca aggaagggtt
    10141 ttgcgacctc gtcggcatgg tgcgtgccgg tatcgccgat cccgagttcg ccaacaaggc
    10201 gcgcgagggc cgcctgggcg agatacgccg ttgcatcggt tgcacccgct gcatcgacga
    10261 ggcctcggaa cccacgacca tgccctacac gccgacctgc tcgatcaatc cggtcatcgg
    10321 caatgaactg cgctggcagc agcagtaccg gccggcgcag gaacccaagc gcgtcgtggt
    10381 ggtcggtggc ggtattgccg gatgcgaggc cgcacgcgtg gcggcgatgc gcggtcaccg
    10441 ggtgatcctg ctcgaacagg gcaagcgcct ggggggccag ctgctgatcg ccgccagggc
    10501 tcccgggcgc gattcgttcg aagaccaggc ctacttcgag gaaaacgaga tggcgcgcct
    10561 gggggtcgac gtgcggctgc agtccgttgc ggatgtggcg gcaatcaagg ccctggcgcc
    10621 cgaggcggtg gtcattgcca cgggctcggt cccgcgcgtg cccgacgatg ttcccggcat
    10681 cgacctgccg cacgtggcgc agggctggga cgtcatgcgc ggcaaggtga gcaccggaga
    10741 gcgcgttgcc gtgatttccc aggaagacta ttaccagacg cccgccgtgg ccgaattcct
    10801 ggccgcccgc ggcaagcagg tggaagtctt ccacaagtcg gtgcaccttg gcacggagat
    10861 cgccaggtat tcgatcggaa tggtgctgag ccgcatggag caatgcggca tccagatcca
    10921 tccgaacctg gtactgaagg agctctggtc ggacggactc gagttcgtct catcctgggg
    10981 cgggaagacc tatcgccggg aaggcttcga cagcgtggta ctggtgtacg gatcgacacc
    11041 gcagcacggg ctgtacgacc agttgaaggc cgaaggctgc atcccgcagc tctacctggc
    11101 cggctcggcg tggcttcctc gtcgcctggc cgaggcgacg cgccatggcg ccggcatcgg
    11161 gctggtgatc tgaagcggga ttcgcgagca accccggtgg cgtacgcgat cgtctacgca
    11221 aagcgcgcga tcacctgccc ggcatagcgc ttgatggcct ccagcttcac gtcgagcggg
    11281 ggctgttgct gcatgatggc tcccacgccc atctcggcca ggatgccggg cacgctccac
    11341 ggcgtcacct gcagatcggt gacgccgata tcctccaggc gccggatgtc gtccaccgtc
    11401 tgggcgtcgg ggcagtgcat catgatgtcg aacggttcgt tctcgcgtcc gaattcccgg
    11461 cgaaagcgtt cgagctgcgc cagcatgtcc gcgaccgcat ccatgtcgtg aatgacgctg
    11521 acccaaccat caccataacg cgccgcacgc cgcagcgcgg gcatcgtggt accgcccacg
    11581 tatatcggaa tgcgcttttt gggtaccggc gccatgatca gggggtcgaa atcgaagtac
    11641 ttgccgtgga attcctgcat cccgccccgc atcagcagac ggatgacctg gatggcctcg
    11701 tcctggcggg caccgcgggt cttcatgtcc acaccgagcc acttgaactc ctccggcatc
    11761 catgcgagcc ccacacccag ggccacgcgg tcgttcgcga tcgcggcgac cgagcacagg
    11821 ctcttggctt ccagcagcgg ctgacggatg gccagcctga gcacggaggg gagaaagcgg
    11881 atgcgcctgg tcacgacggc cattgccgtt atcgcgaccc agggatccag caccggcata
    11941 tcgagcggcc agaagcgcgt gccgtccggc gtgtacggat agggaaccga aatcgtctcg
    12001 ggaaagaacg gggagtccgc cacctggatg ctgtcccagc cgcagtcatc ggccgtgcgc
    12061 gctatatcga ggtgctggtc ggggtcgacc atgaacaggt gaaatccgaa tctcatggct
    12121 tctctcctct tcgcccatgc aggcatcggg acaaacacaa cgccgtggtg cccgctgacc
    12181 aacgaccgtg cccgagcacg tcgggatgct cagcccgtga tcgccgcacg cgcattgcga
    12241 cacgcacggt gaatcacgac gagcaatgcc acgccataga ccagcagcag gaccagcacc
    12301 ggccatatcg acgcaagggc cagcggccac gtcagtccca ccgccagcgc cagccccccg
    12361 agcccgcgca gccagcgctc cgggaacagt tgtcccatgt gacgcagctt gcgaccgacc
    12421 gggacactcg cgcggtaatc gagccacagc aggaaaggca cgatcttgcc cagcatcgcc
    12481 gtgtagatcg gcagcagcgc gccgcacagg aactggccgc ccagcaacac cgggttcagc
    12541 cgcggcaatg cgtccagcca cggcgccagg gcacaaacca gcagcaggtt cacgccgccc
    12601 cacgcccaca gccagcgggc cggatcggcc ccacgcttgc tcgccttcaa cgagcgcagc
    12661 agttgcagca cgaacaacaa taccggcagc accagcaggg ggagcagtac cggccacacg
    12721 gcgagcagga gcagcatcag cgcgttcagc ggaaccagca agcgcgcgag gcgctgcggc
    12781 cacggcggtg tgacgaggaa catcggcacc acggtcagcg ccacgctggc tagcagcggg
    12841 aacacccagc ccatgctgcc gaacagcacg tgcaggcgca gcacctcggg cagcgccagc
    12901 gccacaccgc gcgtcagcac cagcagcagc aatatcccgc atgcgacggt cagccccaac
    12961 ccggccactg accagcgcag gcctgccgtg ctggcatcca ccgcgggact gcgccacaac
    13021 cgcgtgaaca accagcccgt gaacgcgagc aacatcaccc ccaggacggc cccggccgcc
    13081 tgcagcagca atggctgcat cagccacaag cccgctgcaa gaagcgccgt tcccgtctgc
    13141 agcgatcccc acaccagcca gcccgcgagg accgggcgct gcgcacgcgc accggcgagc
    13201 acggcactga tctgcaacag cgcacccagc atgatgttgc cggcgaatcc gagcatcatc
    13261 aggtgcacga tcgccagcgt gacggggtga taccggctcg ccagcacctc cgtcgcgtgc
    13321 cccgcgaaca gcactgcggc gaccagcaac caccatggcg ccgtgcgcag gaagcgtagg
    13381 gggacctcga ccggcaggct gaactcagga ttgatcacgg gaagcgttgc gcctgatcgt
    13441 aacgaccgca cgcccgtcgg gctgcggctc ggtttcccaa tcgcagggac ggcgcgccag
    13501 gtgatcgaac agcgggtagg gcatgcgcgg cagcaggaac gcctggcatg cacccggcgc
    13561 ggccgcatcg agccagtcga gcacgcgatc catgggctca ggcgccgcga gcccgctcag
    13621 gtcgatgcaa tcatccacgg gccgcgttcc tcgcggatgc tcacagctgt ccggcaaact
    13681 ccggcaccgc gcgctcgcac atgggataga ggacgttctc ctccttcatg ttgtgctgct
    13741 gcagcaggat cagcagggtt tcgcagcaac ccacgaaatc cgcggcgttg cgagccgtga
    13801 gcgcctcgtt caggccctcc atgagatcgc gcacctcggc atgctcgatg cgcatcatct
    13861 gcgtcggccc gctgctcatg ccggtggctt tctcgaaagc gggaaacagc ccgttttcct
    13921 ccagggccag atgcgcttcg gtatcggcaa cgaacgccgc cgaggccgcg gcacaggccg
    13981 tccagtcctc gcgccgcgcg gccgcctcgg cctgcgccag cagctcgtcg cagtggcgat
    14041 gctgcgcctg cattttggtg acgataccca tgtgtgcgcc cctgtccgag agaattcccg
    14101 agcagtttac tcggactttc gtgcgattcc ttgtcgcaac tcaaacgtcc ttgaagtagg
    14161 gctcgaccgt gcccttgagt ttcaatgtca ccgcattgcc cttgcggtcg acggcgcgac
    14221 cggccgccac gcgaatccag ccctcgctga cacagtattc ctcgacgttc gtgcgttcct
    14281 tgccgttgaa gcggatgccg ataccgcgcg
//
LOCUS       DAORUQ010000078        14238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4121_length_14238_cov_6.789114, whole genome shotgun sequence.
ACCESSION   DAORUQ010000078 DAORUQ010000000
VERSION     DAORUQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_14238_cov_6.789114"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2826)
                     /locus_tag="PLW13_08490"
     CDS             complement(<1..2826)
                     /locus_tag="PLW13_08490"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQY70451.1"
                     /translation="MSIVKRIASVIAVLIALLILLALGGVGWVVHKTSQHLAPPLDPS
                     GQPSIASYVEPAPVAPAQPPELVSGFSWDTILTPPKSARPWTRWWWPGGDVDAATLTR
                     QLDELDAAGFGGGEVQPFLSGMLNIENEVTWERVYGFDKPPYYQTLDAVMSAAAERGM
                     QLDLTHFSGWPPGGPEINLDDSLTVIAHAAHRVKGGRTVALELPKPGAGASEYIFTAM
                     EFTGADFINFPAANARLLSVLAARVVAGEHGWSPFDLDDTVTLDPASVQVLTARVAGG
                     VLHWEAPPGEWQIIASYLLPSGEVPMGAAQKPQGFVVDHLRKRQVLGHYEYAFGKRTG
                     LPAHYGRGLRGFFNDSLEFRVKRMSVEDILPEFRARRGYDLEPLLPAIYVEGKDNVYF
                     REIVGIHAAPEFELTAQDERIRHDYQKTLSDLVIERFVETSASWAAQRGLVSRGQSYG
                     MDLDILRALGANTIPETEQLWAGGSDLGLKMASSAAALYGRPLVSAESFVWIQRDWTT
                     TPRKLKAAADKLLLAGVNHIVHHGTPYPWTADASKPYGEEGWTPFSGPQNPAHFSSIV
                     GPNNPVLWADVPAINRYIARSQNLLRQGRPAIDVLIYYPFLGFHGANPEAGEPEPLLN
                     GSIPDADPPVDVREDPVLTSGKKTLDAVFTLPPKQEDERDAWVRTLLPLVRELDRRGI
                     SWGWVNDHALQSGKIGARTLTASGGSYQAVLVANAPMIEQQTLHALAGLEAEGVPVVF
                     AGALPERQPGYLDAAQGDREVQQLVSQLIADGAEQRSLDAASLADALQEQILAPVRHL
                     TRGPIRSFRRQLDAQQDILLFANQGATAQTLQLQAEAGQALWWFDAMSGAAWPAETTD
                     GSLSLALLGYESRFLIRGVPMPATLPLRIADSVALQHPARSWPLAEWELAMDGAPGQS
                     TELGDWREDPLLRHARGPGVYTHRFVLD"
     gene            complement(3071..5542)
                     /locus_tag="PLW13_08495"
     CDS             complement(3071..5542)
                     /locus_tag="PLW13_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70452.1"
                     /translation="MKRQPSNSIHCASVGSFSQGTRLRRNALFLGIAASVAVAQPTLG
                     QPAAGGQELEEIIVTATRREQSVQDVAVSITALSGEQLEKLKFFEFEDMAAATPGLSM
                     TSTAREPGVIAVRGIGFAPNSSAPPAVDVYLNELPIDAVYAFTSIYDIQQMELLRGPQ
                     GTLRGRPSPAGAVTMTTRRPDLGAVGGSVSGSFSDADAQNYQGALNVPIVKDTLALRV
                     AGVKNTNEGPGGKLLNGDAPEIDDEGLRATLLFQATDELEFLLTHHYMDRAIDNFTFV
                     EGAGAGYNGPAISADDRRGVAEKMGRQDQEIEITSLQANWDIGEHQLVYIGGYQDLTN
                     EFMQDLDGANAVVDYSSLQHTATDFEVTTHELRFESGGGGRIDYSAGLWYSDTETSTD
                     VHQVAELTGAFGYPPTPRGPADPAYLIGVDIYIPTDAENTAAFGHLEFHVTDKLDIGV
                     GARYLEEKSERSQQLDLGSSLIAIDIGQGEPLTSILCPTILGQVTGLPWQGQTYPNTC
                     DLALAASSFYQPAEEDWDHWVYDASIRYSLSDLTMVYFTAAHSWRPPGVTVGITAPIP
                     DDILFGPPEQSDAFELGLKSEWLDRRLRFNAAMFYQEFDGFIGRFEDVPYVNDNTQTV
                     DEGGFTYNGDAIAYGLEADLFWLISDEWTAQLSMSAQKGEFDDASVPCRDTDFDGKPD
                     NGANPAYADWGASGPVAFCVSDDPISNLPEWNATLQSEYVIPRDALEYYLRGLLNYQP
                     ENDNFTTGFERDSFALMNLYAGVRSSDGRWDVTLWAKNALDEDTVLQLDSEGVVAGFA
                     SGYRGVAVLPEREIGVSVRYAFGSD"
     gene            complement(5708..7030)
                     /locus_tag="PLW13_08500"
     CDS             complement(5708..7030)
                     /locus_tag="PLW13_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HQY70453.1"
                     /translation="MTKSDRNPLRFAFLMVASLFFIWGFCHAMLDVLNKHFQDILHVS
                     KTQSGFIQTSVFGAYFLMALPSALLIRRIGYQRGILVGLAVLAIGAFLFIPAGLVFKT
                     FWSFLLALFVLSSGLACIETAANPYVTVLGPKDAAAGRLSTAQAFNSVAYIIAPTVGG
                     AFIFGQPQQAGASADFHSLVVPYVVLGCVVLLVFGAFSLVKLPGIDGESGESGEAAGA
                     DDPPLHRVPLLRQPHFVWGVGTQFLYVAAQVGVNAFALNYILENAITRDASGAVLTAP
                     LFAWYAGITNAARPEATAAYVITIAMVLYAVGRFSGALITRLIKPHLLLALYGLLNSI
                     VILFVMADIKHVSWMILPLCWLFMSIMFPFIFAMSLRNLGPQTKLAASFLIMSIVGGA
                     ISPPLMGWLADRYDSMAICFVLPLAGFVAPAVYGLLYPRLLEKSARAA"
     gene            complement(7027..8445)
                     /locus_tag="PLW13_08505"
     CDS             complement(7027..8445)
                     /locus_tag="PLW13_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQY70454.1"
                     /translation="MSHSLVFDIGKTNAKALVFDAGLECVYRAQRASEVIAAGSYPHL
                     DVNRLWEWMLGSMRDAAARFRIGRLNVATHGATAALIDPTLGEDGLVLPVLDYEFDGP
                     AGATGYDSLRPAFAETLSPALPAGLNLGRQLWWQQQCFPAEFARTGAILMYPQYWTWR
                     LSGALTGEYTSLGCHTDLWAPRERDYSSLVRSRAWSAKFPRVVSPWVAPASLREVIAR
                     ATGLPGDCAVYAGVHDSNAGFARHLRAAQARPFVLVSTGTWVVCMASAATVQRLDPAR
                     DMLANVDVNGDPVACARFMGGREYARICELTSSDPAQPVTLDQVQATVDSGIFALPDF
                     SGGSGPCAGREGRITGQPAHGAALATLYVALMIELALELLDAPGAVLIEGAFGADPLL
                     CSLVAALCARPVYLSGATDATALGAAMLCHWDIEPEPARREPCEPCAVRGLQAYRARW
                     REGLAPADRVSPTTRKAPGAQR"
     gene            complement(8442..9281)
                     /locus_tag="PLW13_08510"
     CDS             complement(8442..9281)
                     /locus_tag="PLW13_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQY70455.1"
                     /translation="MLEQQRHRLILDLLEERQFVRLQELVEALNASPATVRRDINKLA
                     EEGLLTKIHGGAQLATSEAAARTPRQQLQGAAFLANLERHVAEKRAIAQRAVALCQDG
                     ETILVNGGSSTFMMAEFLCARDINVLTNSFALAGVLLESGKCRVTLPGGELYRKQNII
                     VSAFEDDLIQRYHGSRMFMGTPGIGVHGVMESDPLLIQAEQKLLRQAEQLVVLADSSK
                     IGARGQLVFCPLGEVDILITDSGIDAGSRRLFEEAGIELIVVEPATDASQRDARGPER
                     AIA"
     gene            9437..11533
                     /locus_tag="PLW13_08515"
     CDS             9437..11533
                     /locus_tag="PLW13_08515"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HQY70456.1"
                     /translation="MQPIENLWDDAHAASLDDPQLLLYRSNLLGRDLRITNFGGGNTS
                     AKLPGTDPLSGAEETVLWVKGSGGDLGSMKLDGFATLYLARLEQLKTLYRGLAHEDEM
                     VGYLPHCTFGLNPRAASIDTPLHAFIPHAHVDHVHPDAVIAIACTQRSRELSAEIFGS
                     EIGWLAWQRPGFDLGLKLERLARENPQYCGAVLEGHGLFTWGESSRACYETTLRIVRR
                     AAQWLAAHGRTDAFGGPRHAGITEAAERRRVAAALMPRIRGRISAGQRKVGHFDDSPP
                     VLEFVNSARLGPLAALGTSCPDHFLRTKIRPLVLDFDPAAPDLSAELARCDATLEDAL
                     GGYRADYAAYYQRCARPDSPPMRDANAVIYLVPGVGMFSFARDKATARIAAEFYVNAI
                     NVMREAERVDSYVALPEQQAFDIEYWLLEEAKLQRMPPPKSLAGRVALVTGGAGGIGS
                     ATARRLLGEGACVMLADIDQAALDDITRQLAEAHGADAVRGVICDVTREDSVTRACER
                     LAVEYGGLDILVSNAGIASSAPLEDTSLELWNRNIAILATGYFLVTREAFRLLKRQDC
                     GGAIAFIASKNALVASPNASAYCTAKAAEVQLARAVALEGAPLGIRCNVVNPDAVLRG
                     SKIWGGEWRRERAEAYRMDESELEEHYRQRSLLKRNVFPEDIAEAVYFLVSDRSAKST
                     GNIINVDAGHAPSFTR"
     gene            11547..12836
                     /gene="rhaI"
                     /locus_tag="PLW13_08520"
     CDS             11547..12836
                     /gene="rhaI"
                     /locus_tag="PLW13_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HQY70457.1"
                     /translation="MTLFTPEFIAGQNLTHAAALASDYAALGEQLARSGLDIEAITAR
                     AAAFAVAVPSWGVGTGGTRFARFPGPAEPRNVFEKLEDCAVIQRLVRCTPRVSPHFPW
                     DCVSDHAALREHAAALGLGFDAVNSNTFQDQPGQPLSYRFGSLTHTSAAVREQAIAHN
                     LDCIRYGQALGSRALTVWIGDGGNFPGQQHLVRALERYLDSARTIYAALPADWQMFIE
                     HKCYEPAFYSTVIQDWGTSVLCAMQLGEKARSLVDLGHHAPNVNIEMIVARLVQFGKL
                     AGFHFNDSKYGDDDLDSGSINPYQLFLVFNELVDAQQRQVPGFDPAYLLDQSHNVTDP
                     IESLMNSAATVQRCYVQALLVDRPALGACQDANDALMAGNTLKRAFHCDVSPILAEAR
                     RRAGGAIDPITCYRAARYRERLAPARPAVAGSGSGIV"
     gene            12838..13974
                     /locus_tag="PLW13_08525"
     CDS             12838..13974
                     /locus_tag="PLW13_08525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HQY70458.1"
                     /translation="MARVVTCNEDLRLLAKRRAPRAIFDYVDRGSYDELTIRDNRAAL
                     DRIRLRQRVMVDVSGRSTASTMLGQPVTMPVALAPTGLTGLMHANGEMLAAQAAAEFG
                     VPFALSTMSILSIEQVRAGTREPFWFQLYVMRDRGFVAELIGRAVAAGCPALILTADL
                     QVQGQRHRDLKNGLTVPPKLTMANLLDMALKPRWALGMLGAKGRSFGNLAGYAPSGRG
                     LATLSQWIAGQFDASLDWADVEWVRGRWPGKLIVKGILDPDDARHAVRAGADAVVVSN
                     HGGRQLDGAPAAIDALPGIADALGGEAELLCDSGVRSGQDVLKALALGAHGTMIGKAF
                     LYGLGAMGKAGVTRALELIRNELDVSMALTGVSRLGDVDHNVLW"
     gene            complement(14026..>14238)
                     /locus_tag="PLW13_08530"
     CDS             complement(14026..>14238)
                     /locus_tag="PLW13_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70459.1"
                     /translation="RRDLAPAHCGIRAKIAGPGEPAQDFRVDGPREHGISGLVNLFGI
                     ESPGLTAVLALAEIATAQIADSPRTP"
BASE COUNT         2397 a         4866 c         4698 g         2277 t
ORIGIN      
        1 gtcgaggacg aaacgatgcg tatacacgcc ggggccgcgt gcgtgacgga gcagcggatc
       61 ctcgcgccag tccccgagtt ccgtggactg ccccggcgca ccgtccatcg ccaattccca
      121 ctccgcgagc ggccagctgc gcgcgggatg ctgcagcgcc acgctgtcgg caatgcgcag
      181 gggcaacgtc gccggcatcg gcacgccgcg gatcaggaag cggctctcgt aacccagcag
      241 cgccagggac aagctgccgt cggtcgtctc ggccggccat gccgcgccgc tcatcgcatc
      301 gaaccaccat agtgcctggc cggcctcggc ctgcaactgc agcgtctgcg ctgttgcgcc
      361 ttgattggca aacaggagga tgtcctgctg cgcgtcgagc tggcgccgga agctgcggat
      421 cggtccccgg gtgagatgac gcaccggcgc caggatctgc tcctgcagcg catcggccag
      481 cgacgccgcg tccagggagc gctgctcggc gccgtccgcg atcagttgcg acacgagttg
      541 ctgcacctcg cgatcgccct gcgccgcgtc cagatagccc ggttgacgtt cgggaagcgc
      601 tccggcgaat acgaccggaa cgccctcggc ctcgaggccc gcgagcgcgt gcagcgtctg
      661 ctgctcgatc atcggcgcat tggccaccag caccgcctgg taactgccgc cagaggccgt
      721 cagcgtgcgc gcgccgatct tcccgctctg cagcgcgtgg tcgttgaccc aaccccagct
      781 gatgccgcgc cggtcgagtt ctcgcaccag cggcagcagc gtgcgcaccc acgcgtcgcg
      841 ctcgtcttcc tgcttcggcg ggagcgtgaa caccgcatcc agcgtctttt tgccactggt
      901 cagcaccgga tcctcgcgca catcgaccgg cggatccgca tcgggaatgg agccattcag
      961 cagcggctcg ggctcgccgg cctcggggtt ggccccgtga aaaccgagga acgggtagta
     1021 gatcagcacg tcgatcgcgg gacgcccctg gcgcagcagg ttctggctgc gcgcgatata
     1081 gcgattgatc gccggcacgt ccgcccagag caccgggttg ttcggaccga cgatcgagga
     1141 gaaatgcgcc gggttctgcg gcccgctgaa cggcgtccag ccctcttcgc cgtagggctt
     1201 cgacgcgtca gctgtccacg ggtagggcgt gccgtggtgg acgatgtggt tcacgcccgc
     1261 gagcagcagc ttgtcggcgg cggccttgag cttgcgcggc gtggtggtcc agtcgcgctg
     1321 gatccacacg aaggactcgg cgctcactag cggcctcccg tagagcgccg ccgcggagct
     1381 cgccatcttg agcccgagat cggagccgcc ggcccagagc tgctccgtct ccgggatggt
     1441 gttcgcgccc agtgcacgca ggatgtcgag atccatgccg tagctctgcc cgcgcgagac
     1501 gagcccgcgc tgcgccgccc atgaggcgga cgtctcgacg aagcgctcga tcacgagatc
     1561 cgacaaggtc ttctggtagt cgtggcggat gcgctcgtcc tgcgccgtca actcgaactc
     1621 cggtgccgcg tggatgccga cgatctcgcg aaaatacacg ttgtccttgc cctcgacgta
     1681 gatcgcgggc agcagcggct ccaggtcgta gccgcggcgc gccctgaact ccggcaggat
     1741 gtcctcgacg ctcatgcgct tgacgcggaa ttccaggctg tcgttgaaga agccgcgcaa
     1801 gcccctgcca tagtgcgcgg gcagcccggt gcgtttgccg aaggcgtatt cgtagtgacc
     1861 cagcacctgg cgcttgcgca ggtgatccac gacgaagccc tgcggcttct gcgccgcgcc
     1921 catcggcacc tcgcccgagg gcagcaggta gctcgcgatg atctgccact cgccgggcgg
     1981 cgcctcccag tggagcacac cgcctgcgac ccgggccgtc agcacctgca ccgaggccgg
     2041 atcgagcgtg acggtatcgt cgagatcgaa gggactccag ccgtgttcgc ccgctaccac
     2101 ccgcgcggcg agcacgctga gcaggcgtgc gttcgcggcc gggaagttga tgaagtcggc
     2161 tcccgtgaat tccatcgccg tgaagatgta ttcgctcgcg cccgcgcccg gcttcggcag
     2221 ttcgagcgcg acggtcctgc cgcccttcac cctgtgcgcg gcgtgagcga tcaccgtgag
     2281 gctgtcgtcg agattgattt cgggaccgcc tggcggccag ccactgaagt gcgtgaggtc
     2341 gagttgcatg cctcgctcgg cggccgcgga catcacggcg tccagcgtct ggtagtacgg
     2401 cggcttgtcg aaaccgtaga cacgctccca ggtgacctcg ttctcgatat tgagcatgcc
     2461 gctgaggaag ggctgcacct cgccgccacc gaagcccgcg gcgtcgagtt cgtcgagctg
     2521 gcgcgtgagc gtggcggcat ccacgtcgcc gcccggccac caccagcgcg tccacggccg
     2581 cgccgacttc ggcggcgtga gtatcgtgtc ccagctgaaa ccggagacca actcgggcgg
     2641 ttgcgccggc gccacgggcg cgggctcgac gtaagaggcg atgctcggct gccctgaagg
     2701 atccagcggc ggcgcgaggt gctggctcgt cttgtggacg acccaaccga cgccgccgag
     2761 cgcgagcaag atcagcagcg cgatcagcac cgcgatgacg gacgcgatac gcttcacgat
     2821 tgacatcttg ctcaccctct cgattcgttc gatgtccgac tgaagtcgga cctacgggac
     2881 catgtccgac tgaagtcgga ccttgtacgt ttgctgcggg cgtgttttgt tcatgcccgg
     2941 cttccgggga agccgatcgg caagagcagg aaacgaacga cccgtggcat gactcgacgg
     3001 ccctgcgaca taggagaagc gcgccagcgg tcgccgccaa ggcgtcggcg accgctggcg
     3061 ccgctcggga tcagtcagac ccgaaggcgt agcgcacgct gaccccgatt tcccgctccg
     3121 gcagcacggc cacgccgcga tagcccgagg cgaagccggc caccacgcct tccgaatcga
     3181 gctgaaggac cgtgtcttcg tcgagcgcgt tctttgccca cagcgtcacg tcccagcggc
     3241 catcgctgct gcgcaccccg gcatacaggt tcatgagcgc gaaggagtca cgctcgaagc
     3301 ccgtcgtgaa gttgtcgttc tcgggctgat agttgagcag gccgcgcagg tagtactcca
     3361 gcgcgtcgcg cgggatcacg tactcggact gcagcgtcgc attccactcg ggcaggttcg
     3421 agatgggatc gtcgctcacg cagaaggcca ccgggcccga tgcaccccag tcggcatagg
     3481 ccgggttggc gccgttgtcg ggcttgccgt cgaaatccgt atcccggcac ggcacgctgg
     3541 cgtcgtcaaa ctcgcccttc tgcgcgctca tcgagagctg ggcagtccac tcgtcgctga
     3601 tcagccagaa caggtcggcc tcgaggccgt atgcgatcgc gtcgccgttg taggtgaagc
     3661 cgccctcgtc gacggtttgc gtgttgtcgt tgacgtaggg cacatcctcg aagcggccga
     3721 tgaaaccgtc gaactcctgg tagaacatcg ccgcgttgaa gcgcaggcgt cgatccagcc
     3781 actcggactt cagtccgagc tcgaaggcat cggactgctc gggcggcccg aaaaggatgt
     3841 cgtcggggat cggcgcggtg atccccacgg tgaccccggg cgggcgccag gagtgggcgg
     3901 ccgtgaagta caccatggtc aaatcgctca ggctgtacct gatcgatgca tcgtagaccc
     3961 agtggtccca gtcttcttct gcgggctggt agaacgagga cgctgcaagc gccagatcgc
     4021 aggtattcgg ataggtctgg ccctgccacg gaagcccggt gacctggccc agtatcgtcg
     4081 ggcacagaat gctcgtcagc ggctctccct gcccgatatc gatggcgatg agcgaggagc
     4141 cgaggtccag ttgctgcgac cgctctgatt tctcttccag gtaacgagcc cccacgccga
     4201 tgtcgagctt gtcggtcaca tggaactcga ggtgcccgaa ggccgccgtg ttctccgcgt
     4261 ccgtcgggat gtagatgtcg acgccgatca gatatgccgg gtccgccggc ccgcggggcg
     4321 tcggcgggta gccaaaggcg ccggtgagct ccgcaacctg gtgcacatca gtgctcgtct
     4381 cggtatcgct gtaccatagc cccgcgctgt aatcgatgcg cccgccgccg ccggactcga
     4441 agcgcagttc gtgcgtcgtg acctcgaagt ccgtggccgt gtgctggagc gaggaatagt
     4501 cgacaacggc attggcgccg tcgagatcct gcatgaactc gttcgtgagg tcctggtagc
     4561 cgccgatata gacgagctgg tgctccccga tgtcccagtt cgcctgcagc gaggtgatct
     4621 cgatctcctg gtcctgcctt cccattttct cggccacgcc acggcggtcg tccgccgaga
     4681 tggccgggcc gttgtagccg gcaccggccc cctcgacgaa ggtgaaattg tcgatcgcgc
     4741 gatccatgta gtggtgcgtg agcaggaatt ccaattcatc cgtcgcctgg aacagcaggg
     4801 tggcccgcag gccctcgtcg tcgatctccg gcgcgtcgcc gttcagcagc ttgcccccgg
     4861 ggccctcgtt ggtgttcttg accccggcca ctcgcagcgc cagcgtgtcc tttacgatcg
     4921 gaacgttcaa ggcaccctgg tagttctgcg catcggcgtc cgagaaggat cccgagacac
     4981 tgccgcccac cgcgccgagg tccggccgcc gggtggtcat ggtcaccgcg cctgccgggg
     5041 aggggcggcc tcgcagcgta ccctgcggtc cgcgcagcag ttccatctgc tggatgtcgt
     5101 agatgctggt gaacgcgtat accgcatcga tcggcagttc gttcaggtag acgtcaacgg
     5161 cgggcggcgc cgaggagttg ggcgcaaaac cgatgccgcg caccgcgatc accccgggct
     5221 cgcgtgccgt gctggtcatc gagagccccg gcgtcgcggc ggccatgtcc tcgaactcga
     5281 agaacttcag tttttccagc tgctctccgc tcagtgcggt gatcgagacc gccacgtcct
     5341 gcacgctctg ctcgcgacgg gtcgcggtga cgatgatttc ctccagttcc tgcccccccg
     5401 cggcaggctg accgagggtg ggttgcgcaa cagccacgct ggcagcgatg ccgaggaaca
     5461 gcgcattgcg ccgcagacgc gtaccttgcg agaacgaccc gacggaggcg caatggatcg
     5521 aattggacgg ttgtcttttc atgagttcat ctccctgagt gggtacacat tgtcgttgtc
     5581 agcacacgag acgacgcggc acagcgcgcc acagcaagca gttatggaaa cagccgggca
     5641 aaagcagaac tcgcgacagg cttcgcgccc cgagcgctgc gccgccgttc cccggttcgc
     5701 gagcaattca tgctgcacgc gcggatttct ccagcaagcg tggatacagc agtccataaa
     5761 ccgcaggcgc gacgaatccg gccagcggca ggacgaagca gatcgccatg ctgtcgtaac
     5821 ggtccgcgag ccagcccatg agcggcggag aaatcgcacc gcccacgatc gacatgatca
     5881 ggaacgaagc cgcaagtttc gtttgcgggc cgaggttgcg caggctcatc gcgaagatga
     5941 acggaaacat gatcgacatg aacagccagc acaacggcag gatcatccag gagacgtgct
     6001 ttatgtccgc catcacgaac aggatcacga tcgaattcag gagaccgtag agcgcgagca
     6061 gcagatgcgg cttgatcaag cgcgtgatca gcgcgcccga gaaacgcccg acggcataga
     6121 gcaccatcgc gatcgtgatc acgtaggccg ccgtggcttc gggtcgcgcc gcgttcgtga
     6181 tgccggcgta ccaggcgaac agcggcgccg tgaggacggc gcccgatgcg tccctcgtga
     6241 tcgcgttctc caggatgtag ttcagtgcaa acgcgttcac gccgacctgt gcggcgacgt
     6301 agaggaattg cgtgccgacg ccccagacaa aatgcggttg gcgcagcagc ggcacccggt
     6361 ggagcggggg atcgtcggcc ccggcggcct cgccggattc gccggattcg ccgtcgatgc
     6421 ccggcagctt cacgagcgaa aacgccccga acaccagcag caccacgcag ccgagcacga
     6481 cgtagggcac gaccagcgag tgaaagtccg cggacgctcc ggcttgctgc ggctgtccaa
     6541 agatgaatgc gccgcccacc gtgggcgcga tgatgtaggc cacggagttg aatgcctgtg
     6601 cggtggacag ccggcccgcc gccgcgtcct tcgggccgag caccgtaacg tagggattcg
     6661 ccgcggtctc gatgcacgcc agaccgctgg aaagcacgaa cagcgcgagc aggaacgacc
     6721 agaaggtctt gaagaccaat cctgcgggga tgaacaggaa tgcccctatt gccagcactg
     6781 ccaggcccac caggatgccg cgctggtagc cgatgcgccg gatcagcagg gccgagggca
     6841 gcgccatcag gaagtacgca ccgaagacgg acgtctggat gaaccccgat tgcgtcttgc
     6901 tgacgtgcag gatgtcctgg aagtgcttgt tcagcacatc cagcatggca tggcagaacc
     6961 cccagatgaa aaacaggctg gcgaccatca ggaacgcgaa gcgcagggga ttgcggtccg
     7021 atttcgtcat cgctgcgctc cgggcgcctt gcgcgtcgtt ggcgaaacgc gatcagcggg
     7081 cgcgagaccc tcgcgccagc gtgcgcgata cgcctgcagc ccacgtacgg cgcatggctc
     7141 gcagggctcg cgccgtgcgg gctcgggctc gatatcccag tggcacagca tcgccgcgcc
     7201 gagcgccgtg gcatccgtcg cgcccgacag gtacacgggc cgcgcgcaca acgcggccac
     7261 cagggagcac agcagcggat ccgcgccgaa cgcaccctcg atcagcaccg cgcccggtgc
     7321 atcgagcagt tcgagcgcga gctcgatcat cagcgcgacg taaagggtag ccagcgccgc
     7381 gccgtgcgct ggctgcccgg tgatccgccc ttcccggccg gcacaaggac cgctgccgcc
     7441 gctgaaatcc ggcagcgcga aaatccccga atcgactgtc gcctggacct gatccagcgt
     7501 gaccggttgc gcgggatcgc tcgaggtcag ctcgcagatc ctcgcgtatt cccgcccgcc
     7561 catgaaacgc gcacacgcca ccgggtcccc gttcacgtcg acattggcca gcatgtcgcg
     7621 cgccggatcc agccgctgca ccgtagcggc cgaggccatg cagacgaccc aggtacccgt
     7681 cgataccagc acgaaggggc gtgcctgcgc ggcgcgcaga tgccgcgcga agccggcatt
     7741 gctgtcgtgc acgccggcgt acaccgcgca gtcacccggc aagccggtcg cgcgcgcgat
     7801 cacttcgcgc aggctggcag gagcaaccca cggcgagacg acacgcggga acttcgcgct
     7861 ccaggcccgc gaacgaacaa gcgacgagta gtcccgctcc cgcggcgccc acaggtcggt
     7921 atggcaaccg agcgatgtgt actcgccggt caatgcgccg ctcaggcgcc aggtccagta
     7981 ctgcgggtac atcaggatcg cgcctgtccg cgcgaattcc gcgggaaagc actgctgctg
     8041 ccaccacagc tggcggccga ggttcagtcc ggccgggagc gcgggcgaca gggtttcggc
     8101 gaaggccgga cgcagcgagt cgtagccggt ggcgcctgca ggaccgtcga attcatagtc
     8161 gagcaccggc aggaccagcc cgtcctcacc gagcgtcggg tcgatgagcg ccgcggtggc
     8221 gccatgcgtg gcaacgttca gccgcccgat acgaaagcgc gcggcagcat cgcgcatcga
     8281 cccgagcatc cactcccaca gccggttgac gtcgagatgc ggatagctgc ctgcagcgat
     8341 gacttcgctc gctcgttgcg cgcggtacac gcactcgagc cccgcatcga aaacgagtgc
     8401 cttcgcgttg gtcttgccga tgtcgaagac cagcgagtga ctcatgcgat ggcgcgctcc
     8461 ggtccccgcg catcacgctg cgatgcatcc gtcgccggct cgaccacgat gagttcgatg
     8521 ccggcctcct cgaagagcct gcgcgaaccg gcgtcgatgc cgctgtcggt gatcaggatg
     8581 tcgacctccc ctagcgggca gaacaccagt tgcccgcgcg caccgatctt gctgctgtcg
     8641 gcgagcacca cgagttgttc ggcctggcgc agcagcttct gctcggcctg gatcagcaga
     8701 gggtcggatt ccatcacgcc gtgtacgccg atgcccggcg tgcccatgaa catgcggctg
     8761 ccgtgatagc gctggatgag gtcgtcctcg aaggcgctca cgatgatgtt ctgcttgcgg
     8821 tagagctcgc cgcccggcag cgtgacgcgg cactttcccg actcgagcag gacgccggcc
     8881 agcgcgaatg aattcgtcag cacgttgatg tcacgcgcgc acaggaactc ggccatcatg
     8941 aacgtcgagc tgccgccgtt gaccaggatg gtctcgccgt cctgacacag cgccacggcg
     9001 cgctgggcga tcgcgcgctt ttcggccacg tgccgctcca ggttcgccag gaaggccgcg
     9061 ccctgcaatt gctggcgcgg cgtcctggcg gcggcctcgg acgtcgccag ctgcgcgcca
     9121 ccgtggattt tcgtcagcag cccttcttcg gcgagcttgt tgatgtcgcg ccggacagtg
     9181 gccggcgagg cattcaacgc ctcgaccagt tcctgcaggc gtacgaactg acgttcctcg
     9241 agcaggtcga ggatgaggcg atgacgttgc tgctccagca tgggcttccc gtccgtaccg
     9301 gacctggcag ggattcggcg accttaatcg agtctgatcc gatttgcaat atcttgattg
     9361 cttttgattg cttttggccg tttcgatcgt atagcctagg aacccttacc caacgcctgc
     9421 cgctggagac tcgcccttgc agcccatcga gaatctctgg gatgacgcgc acgcggcctc
     9481 cctggacgac ccgcaactgc ttctctatcg ctcgaacctg ctgggccggg atctgcgcat
     9541 caccaacttc ggcggcggca acacctccgc gaagctgccc ggcacggacc cgctgagcgg
     9601 cgccgaggag accgtgctgt gggtcaaggg ctccggtggc gatctgggca gcatgaagct
     9661 ggacggattc gcgacgctct atctggcaag gctcgagcaa ctgaagacgc tctaccgcgg
     9721 cctcgcgcac gaggacgaaa tggtcggcta cctgccgcac tgcaccttcg gcctcaatcc
     9781 gcgcgccgcc agcatcgaca cgccactgca tgccttcatc cctcacgcac acgtcgacca
     9841 cgtgcacccg gacgccgtga tcgccattgc ctgtacgcag cgcagccgcg agctgagcgc
     9901 ggagatcttc gggagcgaga tcggctggct ggcatggcag cgcccgggct tcgacctcgg
     9961 cctgaaactg gagcggctcg cacgcgagaa cccgcagtac tgcggggccg ttctggaagg
    10021 ccacggcctg ttcacctggg gcgagagttc gcgggcctgc tacgagacga cactgcgcat
    10081 cgtgcgccgc gccgcgcaat ggctggccgc ccacggcagg accgacgctt tcggcgggcc
    10141 acgccatgcg ggcatcaccg aggccgccga acgacgccgc gtcgccgccg cgctgatgcc
    10201 gcgcatccgc gggcgcatca gcgccggaca gcgcaaggtc gggcacttcg acgactcgcc
    10261 cccggtactg gagttcgtga attccgcgcg cctcgggccg ctcgccgcgc tgggcacctc
    10321 ctgcccggac cacttcctgc gcaccaagat ccggccgctg gtactcgatt tcgatcccgc
    10381 ggcgccggat ctttcggccg agctcgcgcg ctgcgacgcc acgctcgaag acgctctcgg
    10441 cggctatcgc gcggattacg ccgcgtacta ccagcgctgc gcgcggcccg acagcccgcc
    10501 gatgcgcgat gccaatgcgg tgatctacct cgtgcccggc gtgggcatgt tcagcttcgc
    10561 acgcgacaag gccaccgcac gcatcgccgc cgagttctat gtaaacgcga tcaacgtgat
    10621 gcgcgaggcc gagcgcgtcg acagctacgt ggcgttgccc gaacagcagg ctttcgacat
    10681 cgagtactgg ctgctcgagg aggccaagct ccagcgcatg ccgccgccga agagcctcgc
    10741 gggccgcgtg gcgctcgtga ccggcggcgc gggcggtatc ggcagcgcga ccgcgcgccg
    10801 gctgctcggg gaaggcgcct gcgtcatgct ggccgacatc gaccaggccg cgctggacga
    10861 catcactcga cagcttgccg aggcgcacgg cgccgacgcc gtgcgcggcg tgatctgcga
    10921 cgtgacgcgg gaggactccg tgacacgagc ctgcgagcgg ctcgccgtag agtacggcgg
    10981 gctcgatatc ctggtatcga acgccggcat cgcgagctcc gcaccgctcg aggacacgag
    11041 cctcgaactg tggaaccgca acatcgccat cctcgccacc ggctactttc tggtcacgcg
    11101 cgaggccttc cggttgctga aacgccagga ctgcggcggc gcgatcgcct tcattgccag
    11161 caagaacgcg ctcgtggcct cgccgaatgc ctcggcgtac tgcaccgcca aggcggccga
    11221 agtgcaactg gcgcgcgcgg tcgcgctcga gggcgctccg ctcggcatcc gctgcaacgt
    11281 ggtcaatccc gacgccgtgc tgcgcggctc gaagatctgg ggcggcgagt ggcggcgcga
    11341 acgcgccgag gcctaccgca tggacgagag cgagctcgag gagcactacc gccagaggag
    11401 cctgctcaaa cgcaacgtct tccccgagga catcgccgag gcagtgtatt tcctggtctc
    11461 ggaccgctcc gccaaatcga ccggcaatat catcaacgtc gacgcgggac atgctccctc
    11521 gttcacgcgc tgagaggaaa cgcaacatga ccctcttcac ccctgaattc atcgccgggc
    11581 aaaacctgac tcacgccgcc gcactcgcca gcgactatgc cgcgctcggc gagcaactag
    11641 cgcgctcggg attggacatc gaggccatca ccgcgcgcgc cgccgccttc gcggtggccg
    11701 tgccgagctg gggcgtgggc accggcggca cgcgtttcgc gcgtttcccg gggcccgccg
    11761 agccacgcaa cgtgttcgag aaactcgagg actgcgccgt gatccagcgc ctggtgcgct
    11821 gcacaccgcg cgtctcgccg cacttcccct gggactgcgt gtcggaccat gcggcgctgc
    11881 gcgagcacgc cgcggcactg ggactgggct tcgacgcggt gaactcgaac accttccagg
    11941 accagccggg acagccgctg tcataccgtt tcggctcgct gacgcacacc agcgccgcgg
    12001 tccgcgagca ggcaatcgcg cacaacctcg actgcatccg ctacggacag gcgctgggtt
    12061 cgcgcgcgct gaccgtgtgg atcggcgacg gcggcaattt ccccgggcag cagcatctcg
    12121 tgcgcgcgct ggaacgctac ctcgattccg cgcgcacgat ctacgccgcg ctgcctgccg
    12181 actggcagat gttcatcgag cacaagtgct acgagccggc cttctactcc accgtgatcc
    12241 aggactgggg caccagcgtg ctctgcgcca tgcagctcgg cgagaaagcc cgttcactgg
    12301 tggatctcgg tcaccacgca cccaacgtca acatcgagat gatcgtggcg cggctggtgc
    12361 agttcggcaa gctcgcaggc ttccacttca acgacagcaa gtacggtgac gacgatctcg
    12421 acagcggctc gatcaaccct taccagctgt tcctcgtgtt caacgagctg gtcgatgccc
    12481 aacagcgcca ggtgccgggc ttcgatcccg cctacctgct ggaccagtcg cacaacgtca
    12541 ccgaccccat cgagagcctg atgaactcgg cggccaccgt gcagcgctgc tacgtgcagg
    12601 cattgctggt ggaccgccca gctctcggtg cctgtcagga tgcgaatgac gcgctgatgg
    12661 ccggcaacac cctcaagcgc gccttccact gcgacgtctc gccgatcctg gcggaggcgc
    12721 gccggcgcgc cggcggcgcg atcgacccga tcacctgcta ccgcgcggcg cgctaccgcg
    12781 agcgcctcgc gccggcccga cccgccgtcg cgggcagcgg ctcgggcatc gtctgacatg
    12841 gctcgcgtcg ttacctgcaa cgaggacctg cggctgctcg cgaaacgccg cgcgccgcgc
    12901 gcgatcttcg actacgtcga ccgcggctcc tacgacgagc tcacgatccg cgacaatcgc
    12961 gcggcgctgg accgcatccg gctgcgccag cgcgtgatgg tcgacgtctc cgggcgcagc
    13021 accgcgagca cgatgctggg ccagccggtc acgatgccgg tggcgctggc gccgaccggg
    13081 ctgacgggat tgatgcacgc caacggcgag atgctcgcgg cgcaggccgc cgcggaattc
    13141 ggcgtgccct tcgcattgag cacgatgtcg atcctctcga tcgagcaggt gcgtgcgggt
    13201 acgcgcgagc ccttctggtt ccagctctac gtgatgcgcg accgcggctt cgtggccgaa
    13261 ctgatcgggc gcgcggtggc ggccgggtgc ccggcgctga tactcaccgc cgacctgcag
    13321 gtgcagggac aacgccaccg tgatctcaag aacgggctca cggtgccccc gaagctgacg
    13381 atggcaaacc tgctcgatat ggcgctgaaa ccccgctggg cgctgggcat gctcggggcg
    13441 aagggccgca gcttcggcaa tctcgccggc tatgcgccga gcggacgcgg gctcgccacg
    13501 ctgtcgcaat ggattgccgg acagttcgat gcctcgctcg actgggccga cgtcgagtgg
    13561 gtgcgcggcc gctggccggg caaattgatc gtcaagggca tcctcgatcc cgacgacgct
    13621 cggcacgcgg tgcgtgccgg tgccgatgcg gtcgtggtct cgaaccacgg cggacgccag
    13681 ctcgacggcg cgcctgcggc gatagacgca ctgcccggca tcgccgacgc gctgggcggg
    13741 gaggcagaac tgctctgcga cagcggcgtg cgcagcggcc aggacgtgct caaggctctg
    13801 gcgctcgggg cacacggcac gatgatcggc aaggcgttcc tgtacggcct cggcgccatg
    13861 ggcaaggccg gcgtcacgcg cgcgctcgaa ctgatacgca acgaactcga cgtctcgatg
    13921 gcgttgacgg gcgtgagccg gttgggcgat gtcgatcaca acgtgctctg gtgaaccagg
    13981 aattcaggaa gagctcagga tgcaggccgg cgccacgcgc gaatttcagg gagttcgcgg
    14041 agaatcggcg atctgcgccg tggcgatctc cgccagcgcc agcaccgcgg tgagccccgg
    14101 cgactcgatg ccgaacaggt tcaccagccc ggagatgccg tgctcgcgcg gaccgtcgac
    14161 gcggaagtcc tgcgcgggct cgccggggcc ggcgatcttc gcccggatgc cgcagtgcgc
    14221 aggcgcgaga tcgcgcct
//
LOCUS       DAORUQ010000079        14076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4188_length_14076_cov_5.953570, whole genome shotgun sequence.
ACCESSION   DAORUQ010000079 DAORUQ010000000
VERSION     DAORUQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14076
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_14076_cov_5.953570"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLW13_08535"
     CDS             <1..208
                     /locus_tag="PLW13_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70460.1"
                     /translation="GHDGMVRHAKSDFRGPAFESDVTFIDGEVVDKIENSQWGFPVVQ
                     VKVRMSNQAGTTVVTSLNEVELPY"
     gene            complement(252..1013)
                     /locus_tag="PLW13_08540"
     CDS             complement(252..1013)
                     /locus_tag="PLW13_08540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70461.1"
                     /translation="MDTGLKGKLVLVTGGAAGLGRAIALAFAEQGANLILVDINEQGL
                     ADASGEIRNLGVNCETHKLDLSVENDIKAFGAKICTDHGALDVLINNAGLAYGEIAQG
                     FEHLSLEKWLSFLTINSIAPLILGQALRPVLSRARGLIINQSSMAANVPATAYGVTKA
                     TLNSITFGMANQFGADGIRVNAVEPGIMETPANKAGLPDETYTRVQGMQLLPLHGTAE
                     DIANLHVFLASKQGRFINNEIVLCDAGNRLRGWRH"
     gene            1171..3249
                     /locus_tag="PLW13_08545"
     CDS             1171..3249
                     /locus_tag="PLW13_08545"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HQY70462.1"
                     /translation="MADQIVITEKSSQAKDVRTAVGSRYGDILSAEGHLFELVEPEDV
                     VPAWKRWAPILLRPEGLYGTRPAQGGNKAAKLKAIREALRTARRVWLATDCDREGQLI
                     GQEILEHYNYRGQVMRVLFTAQDEQTIRDAFARARPNSEYARLYAAAVARRQADQIYN
                     LSLTRTATVILGRGARGVIGIGRVKTPTLAIVCRRELEIRDFVPVSYFEIVARARVEG
                     GEFRMRHAPPERIVRREKAEEVVNAARGFVGTLGVRVEEKRQGPPRLHDLPSLQKFCG
                     SSFGWPAAKTLDVAQQLYDGQGKKIITYPRAEVRYLPESLIADVPRIVAALQVGQSFS
                     AIPVPTPPVIRKGANGSFHDKGVQGASHHAVIPNVNTVDELREIWPRLSPDEKRLFDL
                     IARAYLAAVMPDFQYRQTSAVLDVRGFAFRASGRQPIVPGWRAAFPQWQPAEEKGDEA
                     QLLPALRNGETAQLRDPVIEDKETRPPPRYNEGTLIEAMQNAWRFVDDEVLRDRLKQA
                     KGIGTPATRAEIIGGLKKQGFLVVEGKHIVPSASGLGLFGVLERADPALVDPGVTAQL
                     ECLLDDVLTGRQEMVDAIDAVCDVARRIIGKLKEGAVAGGASLPGAAAGGAASDCAPT
                     PAMKRFAASIAREKHIKPPRGYTRSASICRSFLEQHAPGKAVTAESKRSESSHDAKRG
                     KRGRRITRKQ"
     gene            complement(3313..3669)
                     /locus_tag="PLW13_08550"
     CDS             complement(3313..3669)
                     /locus_tag="PLW13_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70463.1"
                     /translation="MNGSDFAYLRHRAGFDQRAAAQFLDVSVRTVRRYETTGAPRMAV
                     LALAARAGEYPGWLGYRFHDGALLTPLGDRVTQTEIEQRDYLVYLAWWRGWEAHRKGE
                     PEPAPKRPALHIVECA"
     gene            4071..4874
                     /locus_tag="PLW13_08555"
     CDS             4071..4874
                     /locus_tag="PLW13_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY70464.1"
                     /translation="MDTSIFSSILEHYQGLLSVYTAVGIMAVVFIGKRLLLLIPAIRQ
                     TQALNKKACEERNTRSFYPRVQKRVRVWGLLTDVVIFALVLPFCLTLEAQPWWRMLLD
                     IFAILMVYDFFYYLTHRFLFHDGPLGGPLIWVHAVHHQQRNPCRADSSYLHPLEPVLG
                     LALYAATIAVLAAVIGRFHVATIVITWVAFSEINLHNHDLMEEDRFPFRYLKYMSFMH
                     HVHHARFTAGNFATISLFYDWLFGTYDTGGGYKKKAVVAGADSPSREAR"
     gene            4987..6933
                     /locus_tag="PLW13_08560"
     CDS             4987..6933
                     /locus_tag="PLW13_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HQY70465.1"
                     /translation="MPGIGKLIQGCLLLAAVMTAQAASAAESAVDQHQLGTKYSPLTQ
                     INKDNVANLELAWEYHTGDLPPADLKNLLIAFEDQPSLIEGNLVVCSTTRRVIALDPQ
                     TGKERWSFDPQDPLESKKIGMRKCRGVSNWIDREAADGAACKSRIFLGTADYRLVAID
                     AKNGKPCEGFGDNGVVRMPASKPELFPGELVATSHPAVVNDVLVVGSAVADNQRVDAP
                     SGRVLAFDARTGKQLWEFDPVPRDPADPAARSWARGTGEGYGGGNVWSSMAVDQELDL
                     VYLPTTSPSGDFYGGDRAGDNNYTTSVVALRGATGEVAWHFQVVHHNVFDFDVPSQPM
                     LIDFPHNGAMVPALVQNTKMGLIFVFDRATGVPLVPIEERPVPQDVKVDGEVLSPTQP
                     FPVGMPALGPLGFSPEDAWGFTPVDKWLCRERIKELNHGPIYTPPSEKGTIFSPSVGG
                     GPNWGGGAYDPQSHIMVVPSNRVPVVVTLIRRENARLDETQAIEAGAAMTFANAGAPY
                     VPKVEPLLSPLGAPCSEPPWAALTAVDLAKGEIVWDVPLGSIEKLMPIPIPWEAGTPG
                     AGGPLLTAGGLVFIGFTLDDKLRAFSLDTGEVLWSADLPAAGTAIPVTYEAGGEQYVV
                     IAAGGHSMFGTTLGDSVVAYKLKR"
     gene            complement(6964..8193)
                     /locus_tag="PLW13_08565"
     CDS             complement(6964..8193)
                     /locus_tag="PLW13_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY70466.1"
                     /translation="MSKCPYADLSDPDLHRGGVPEELYRQLREARVVRQDAPMRGGEG
                     FWGFFLQEDVDRISKNPALFSSAAKSAFLNDVPEDQLPLMRTMILNMDPPEHVKYRRI
                     VRNAFTPSKVDSYAPRFREVVREAIDRIVARGECEFVTDVACVLPLITICEILGVPIE
                     DRTKFFEWTNTMLGADDPDLNLSELDGQMAAAELYMYADTIMAHHRDDPQDDIVGALL
                     RGSVGGEKLNEDEFRSFMLLLIVAGNETTRTMTSQGMRLLMEHPEQYRMMVEDPTLIP
                     DAVEEILRFNPAVINFRRTVTQPTEVGGQALAAGDKVVMFYQAASRDEAVFENPDCFD
                     ITRPRREDVRNNHRAFGIGEHFCLGSHLARLQLNIVFEELTKRIRNPRLDGEIKWLRS
                     NFIHGIKEMPMKFDVAV"
     gene            8378..8818
                     /locus_tag="PLW13_08570"
     CDS             8378..8818
                     /locus_tag="PLW13_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY70467.1"
                     /translation="MRYLHTMIRVASLDDALDFYCRKLGLAQQRRYDNEAGRFTLVYL
                     AAPQDADAAEQLSAPQLELTWNWDPEVYTGGRNFGHLAYEVADIYALCRRLADAGVTI
                     NRPPRDGRMAFVRSPDGISIEFLQRGAALPPQEPWLSMPNTGSW"
     gene            8969..9409
                     /locus_tag="PLW13_08575"
     CDS             8969..9409
                     /locus_tag="PLW13_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQY70468.1"
                     /translation="MAYWYWLAFGMALMAVEIFLPSFTALWFGVGAIIVGVLLLALPG
                     MTLAWQVLIWALASAAFTFAWFRFFRNKAPDRTKAGMGREALLGETAMVVHAPLGEKR
                     GLLRFATPKLGSDEWQFICNQSVAEGDRVVVDDVSGNTLIVSKR"
     gene            9445..10290
                     /locus_tag="PLW13_08580"
     CDS             9445..10290
                     /locus_tag="PLW13_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HQY70469.1"
                     /translation="MESLFPVLVFFALVFVTIAKLACIVPQGSKWVVQRLGKYHKTLN
                     PGLSFIIPFFDVVAYRVTTKDIVLDIPSQEVITRDNVVIIVNAVAYINILQPEKSVYG
                     VEDFRIAIQNLVQTSLRSIIGEMDLDDALSSRDQIKARLKEAISDDISDWGITLKTVE
                     IQDINPSDRMQAAMEEQAAAERQRRATVTRAEGSKSAAILEADGRLEASRRDAEAQVV
                     LAEASQRAIEMVTTAVQDKELPVMYLLGERYIQSVKDMAVSPNAKLVLIPADLPASIR
                     GMIGK"
     gene            complement(10291..11814)
                     /locus_tag="PLW13_08585"
     CDS             complement(10291..11814)
                     /locus_tag="PLW13_08585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQY70470.1"
                     /translation="MPPLDRRGFLAGAGLAAALGASGCAGTQGDRGQPRFAHGVASGD
                     PGAERVVIWTRLDGAGAAEELEWLVAREPQLRHVVRRGRVVADPLRDFCCKVDVGGLE
                     PGTTYFYAFRVHGEHSVVGRTRTLPQDAVENLRFAVAACANYQQGSFAAYARIAELES
                     LDAVLHLGDYIYEYGDQTDGRLPLDPPHEILTLEDYRRRYAFYRRDPALQACHRAHPF
                     IAVWDDHESANNAWRDGAGNHDPEREGPWRERRAAAIQAWHEWMPVRDTAADAPIHRS
                     FRFGDLADLVMLDTRLEGREQPVASPQDIASNPARELLGAAQEAWLLGELDASQARGT
                     RWRLLGQQVVFAPLGNFNPDQWDGYPQARQRILQHLGRAGIDNVVFLTGDIHSSWALD
                     VAPDPYDRARYDGQSGRGAQAVEFVTPGIASEPLGHYLARRDPEAHARMVEGIKQQPH
                     LRFADYTNRGFVSLEVNAQRVEASWHFVAAPTDPQSRVAIAHRETVRAGENRLSRPV"
     gene            complement(11814..12956)
                     /locus_tag="PLW13_08590"
     CDS             complement(11814..12956)
                     /locus_tag="PLW13_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQY70471.1"
                     /translation="MGPLSGKKIVEIAGIGPAPFAAMMLADMGAEVIRVSRPSGSMLS
                     MAQNEKLDFGNRGKRCIALNLKDPHAADVVLRLVESADAIIEGYRPGVMEKLGLGPEV
                     CLARNPRIVFGRMTGWGQDGPLGNAAGHDLNYIALTGALHAIGEKGQKPVVPLNLIGD
                     FGGGGMLLAFGVVCAMLEAQASGKGQVVDAAMVDGASALMTMMYGAFQSGFWHNTRGS
                     NMLDGGAHFYGVYETADGRYVSIGSIEPQFYALLLEKLGLAGEELPHQHDARHWDALK
                     QRFAEIFRSRTRDEWCRIMEGTDICFAPVLGFDEVTSHPHIKARGTFIEDDGVWQPAP
                     APRFSRSAPERPAPAVAIGADTDVLLAEAGFSAEQIARMRAAGTVA"
     gene            complement(13050..13352)
                     /locus_tag="PLW13_08595"
     CDS             complement(13050..13352)
                     /locus_tag="PLW13_08595"
                     /inference="COORDINATES: protein motif:HMM:NF022662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3012 domain-containing protein"
                     /protein_id="HQY70472.1"
                     /translation="MRMNTTIRTGALILLAALAAACEPEVGSARWCEALAQKPKTDWS
                     LGEAKDYAKHCLLQATEVGGERWCRKLADKPKGEWSTDEAASYAKHCLIKGSGGEG"
     gene            complement(13349..>14076)
                     /locus_tag="PLW13_08600"
     CDS             complement(13349..>14076)
                     /locus_tag="PLW13_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQY70473.1"
                     /translation="QRELPRLALLAPRYEDDSLCIEAPGRDPLRVPLAYRAAESFTAQ
                     VWSDACEVLDAGSEASRWLQAAVQPERALRLVRMRRGFRRRLARAPLLGDATTTAFAD
                     AAPFLVVNMASLRALNAALAQRGQPAVGMERFRPNIVVEGLDAFREHALAALQRDEGS
                     FRLCYPCERCAVITVDPALGVRDEPGRAAFAMLAAMNPLPGTAKPLFGINATWQGGEG
                     TSLGVGDVFVARESTQEPQGDDP"
BASE COUNT         2434 a         4667 c         4558 g         2417 t
ORIGIN      
        1 gggccacgac ggcatggtgc gccacgccaa gtccgatttc cgcggcccgg ccttcgaaag
       61 cgacgtgacc ttcatcgacg gcgaagtcgt cgacaagatc gagaactcgc aatggggttt
      121 cccggtcgtc caggtaaagg tcaggatgag caaccaggcg ggtacgacag tggtgacttc
      181 gctcaacgag gtcgaactgc cgtactgagg gccccgccac ggtcaggcgg gcgcagcgtt
      241 caggcagttt ttcagtggcg ccagccacgc aggcggttgc cagcatcgca cagcacgatc
      301 tcattgttga tgaagcgccc ctgtttcgac gccaggaaca catggagatt ggcgatgtct
      361 tcggcagtgc cgtgcagcgg cagcaattgc atgccctgga cacgcgtata ggtctcgtcg
      421 ggcagtccag ccttgttggc gggtgtttcc atgatgcccg gctcgaccgc gttcacgcgg
      481 atgccatcgg cgccgaactg gttggccatg ccaaaggtga tcgaattcag cgtcgccttg
      541 gtgacgccgt aggccgtggc gggcacgttg gccgccatgg aggactggtt gatgatgaga
      601 cccctggccc tggagaggac ggggcggagc gcttgcccca ggatcagggg cgcgatgctg
      661 ttgatggtga ggaaggacag ccacttttcg aggctcagat gctcaaagcc ctgggcgatc
      721 tcgccgtacg cgaggcccgc attgttgatg agcacgtcga gggcgccatg gtcggtgcaa
      781 atcttcgcgc cgaacgcctt tatgtcattt tcgacggaca gatctagctt gtgggtttcg
      841 cagttcacac ccagattcct gatctccccc gacgcatcgg cgagcccctg ttcgttgatg
      901 tcgacaagga tgaggtttgc gccctgctcg gcgaaggcaa gcgcgatggc gcggcccagc
      961 ccagccgcgc cgccggtcac cagcaccagt tttcctttca agcctgtgtc cattttccgc
     1021 tcccggcatg cgaatcgagg gttatcgctc tgttgatgag catccagcgt agacgatact
     1081 ctcgccaaag cgagttgcaa ccgtcacgtc ggcaggcggg cacaagggca acgtcagacc
     1141 gaacccccgt gcgatgtaac cggaccatcc atggctgatc agatcgtcat caccgagaaa
     1201 agcagccagg cgaaagatgt ccgtaccgcg gtgggttctc gctacggcga catcctttcg
     1261 gccgagggcc atttgttcga gctggtcgaa cctgaggatg ttgtgccggc ctggaagcgc
     1321 tgggcaccga tcctgctgcg ccctgaaggg ctctatggca cgcgcccggc acaggggggc
     1381 aacaaggccg ccaagcttaa ggccattcgc gaggcgctgc gcacggcgcg gcgggtgtgg
     1441 ctggcgaccg attgcgatcg cgagggccag ctcatcggcc aggaaattct cgagcactac
     1501 aactaccgcg ggcaggtcat gcgggtgctg ttcaccgccc aggacgagca gaccatccgc
     1561 gatgcgttcg cccgggcacg acccaatagc gaatatgccc gcctgtacgc cgccgccgtt
     1621 gcgcgccgcc aggccgatca gatctacaac ctgtctctga cccgcaccgc gaccgtcatc
     1681 ctgggccgcg gtgcgcgcgg cgtgatcggg attggtcgcg tgaagacgcc gacccttgcc
     1741 atcgtgtgcc ggcgcgagct ggaaatccgg gatttcgtgc ccgtttccta tttcgagatt
     1801 gtcgcgcgcg caagagtgga gggtggcgaa ttccggatgc ggcacgcgcc gcccgagcgc
     1861 atcgtgcgac gggagaaggc ggaggaggtg gtcaacgccg cccgcggttt tgtgggcacg
     1921 ctcggcgtgc gcgtcgagga aaagcgccag ggaccgccca ggctgcacga tctgccgtcg
     1981 ttgcagaaat tctgcggctc gagtttcggc tggccggcag ccaagacgct cgacgtggcg
     2041 cagcagctct acgacggcca gggcaagaag atcatcacct atccgcgcgc cgaggtgcgc
     2101 tacctgcctg aaagcctgat cgcggatgtg ccgcggatcg tggccgcact gcaggtgggc
     2161 cagtcgttca gcgcgatccc cgtgcccaca ccgccggtaa tccgcaaggg cgcgaacggc
     2221 agcttccatg acaagggtgt gcagggggcg agtcaccatg ccgtgattcc gaacgtcaac
     2281 acggtcgacg aactgcggga gatctggccg cgcctttcgc cggacgagaa gaggctgttc
     2341 gacctcattg cgcgggcgta cctggccgcc gtgatgcccg attttcaata ccgccagacc
     2401 agcgccgtgc tcgacgtccg cggcttcgca tttcgcgcct ccggccgcca acccattgtt
     2461 cccggctggc gcgccgcgtt cccgcaatgg cagcccgcag aggagaaggg cgacgaggcg
     2521 cagctgcttc cggcgctgcg taacggcgag accgcacaac tgcgcgatcc ggtcatcgag
     2581 gacaaggaaa cccggccgcc gccacgttac aacgaaggca ccctgatcga ggcgatgcag
     2641 aacgcctggc gcttcgtcga cgacgaggta ctgcgcgatc gcctcaagca ggcgaagggt
     2701 atcggaacgc cggcgacccg cgccgagatc atcggcggtc tgaagaagca ggggttcctc
     2761 gtggtcgagg ggaagcacat cgttcccagc gcgagcggcc tgggtctgtt cggtgttctc
     2821 gagcgggctg atccggcgct ggtagacccg ggagtgacgg cgcaactcga atgcctgctc
     2881 gacgatgtcc tgaccggcag gcaggagatg gtcgatgcga tcgatgccgt gtgcgatgtc
     2941 gcccggcgca tcatcggcaa gctcaaggaa ggtgccgttg cgggaggagc atccttgcct
     3001 ggtgccgcag ccggtggcgc ggcgagtgac tgtgcgccaa cgcccgcgat gaaacgtttc
     3061 gccgccagca tcgctcgcga aaagcacatc aaacccccgc gaggctacac gaggtccgca
     3121 tccatctgcc gctcgttcct cgagcaacat gcgcccggga aagcggtaac agccgagtcg
     3181 aaacggagcg aatcgagcca cgatgcgaag cgcggcaagc gcgggcgcag gatcacccgc
     3241 aagcagtgac tccaacggca ggccaagctg ccatcgctga tccggccaat actgcattta
     3301 ccataatata cattatgcgc actctacgat gtgcagagca ggccgcttcg gggccggctc
     3361 cggctcaccc tttcggtgcg cttcccatcc acgccaccac gccaggtaga ccagatagtc
     3421 gcgttgctcg atctcggtct gcgtcacccg gtcacccagc ggcgtcagca gcgcaccgtc
     3481 gtggaaccga taaccgagcc agcccgggta ttcaccagcc cgcgcagcca gcgccaacac
     3541 ggccatgcgc ggtgcgccgg tcgtttcgta acggcgaacc gtgcgcacgc tgacatcgag
     3601 gaattgcgcc gccgcccgtt gatcgaagcc ggcgcgatga cgcagatacg cgaagtcgga
     3661 accgttcatg tccggtgtcg tcccggatcg gctcacgcgc ccggcctgcg gccggatgcg
     3721 tgatcctcac caggacaaac tgtccgctga tcaggccgag gccgcgcgct gcaattcggg
     3781 cccggatcgg tcaccgcccg ccgctcgatt tggtccctcg tgcacacgaa cgtttgcgcg
     3841 tacccctgtt tcagttgatg tatgtcaacg ctctggttcg ccgacgaccc tgactctctt
     3901 gacatgctaa gagccgcgga ttagcttggt gagaacaacg ttctctctag cgaagaacaa
     3961 ggcaccgggc gcaagctcat ggatatattc accggtagtc agcacgtggt ccgcagcacg
     4021 gtacgtgagg tggcgctgcc gtcaatcgga accacagggg acaagccgcc atggacacat
     4081 ccatattttc ttcgatcctg gagcactacc aggggttgct gtccgtctat acggccgtgg
     4141 gaatcatggc ggttgtattc attggcaagc gcctgctgtt gctgatcccg gctataaggc
     4201 aaacccaggc gctgaacaag aaggcttgcg aagaacgaaa cacgcgcagt ttctacccgc
     4261 gtgttcaaaa gagagtccgc gtctggggcc tgctgaccga cgtggtgatc ttcgcgctcg
     4321 tactgccctt ttgccttacc ctggaagccc aaccctggtg gaggatgctg ctggatatct
     4381 tcgccatcct gatggtgtac gactttttct actacctgac gcatcgcttc ctgttccacg
     4441 acggcccgct gggtggaccg ctgatctggg tccacgccgt gcaccatcaa caacgtaatc
     4501 cctgccgggc cgattccagc tatctgcacc cgctggaacc cgtgctgggg ctcgcacttt
     4561 acgccgccac cattgccgtg ctggctgcgg tcataggcag attccacgtc gcgaccatcg
     4621 tgatcacctg ggtcgcgttc tccgagatca acctgcacaa ccacgatttg atggaggaag
     4681 atcgtttccc gttcaggtac ctgaagtaca tgtcgttcat gcatcacgtc caccacgcgc
     4741 ggttcacggc gggtaacttt gccaccatat cgctgttcta cgactggctg tttggcactt
     4801 acgacaccgg gggcggctac aagaaaaaag cagtggtcgc cggtgcggat tccccctcgc
     4861 gggaggcgcg ttgatgaatt ggctgcagaa gcatgacgcc aattgacgcc gcctctaccg
     4921 gcggccacaa tgcccgctta tccacgacgc ctagcattgc ccatgagatc gatagacgaa
     4981 ccccgtgtgc ccggaatcgg caagctgatc caaggctgcc tgctgttggc ggcagtgatg
     5041 actgcgcagg ccgccagcgc cgcggagtcg gccgtggatc aacaccagct cggcacgaaa
     5101 tattcgccgc tgacgcagat caacaaggac aacgtggcga atctcgaact ggcctgggag
     5161 tatcacaccg gcgatctccc gcccgccgat ctgaagaacc tgctgatcgc ctttgaagac
     5221 cagccgagcc tgatcgaggg caacctggtg gtttgctcca ccacgcgccg ggtgattgca
     5281 ctggatccgc agaccggcaa ggagcgctgg agctttgacc cgcaggatcc gctggagagc
     5341 aagaaaatcg gcatgcgcaa atgccgcggt gtgtcgaact ggatcgaccg cgaggccgct
     5401 gacggcgccg cctgcaaatc gcgcattttc ctcggcaccg ccgattaccg gctggtggcc
     5461 atcgatgcca agaacggaaa gccgtgcgaa ggcttcggcg acaacggtgt cgtgaggatg
     5521 cccgcgagca agccggagct gttccccggc gaactggtgg caacctcgca tccggccgtg
     5581 gtcaacgatg tgctggtcgt cggttcggcc gtcgccgaca accagcgcgt ggatgccccc
     5641 agcggccgcg tgctggcctt cgatgcccgc accggcaaac agctgtggga gttcgacccg
     5701 gtgcctcgcg atcctgccga tccggccgcc cggagctggg ccaggggcac gggcgaaggc
     5761 tacggcggcg gcaacgtctg gtcgtcgatg gcggtcgatc aggagctcga tctcgtctac
     5821 ctcccgacca ccagcccgtc gggcgatttc tatggcggcg accgtgccgg cgacaacaat
     5881 tacacgacct cggtcgtcgc gctgcgcggt gcaaccggcg aagtggcgtg gcacttccag
     5941 gtcgtgcacc acaacgtgtt cgatttcgac gtgccgtcac agccgatgct gatcgacttc
     6001 ccgcacaacg gcgcgatggt tccggcgctg gtgcagaaca ccaagatggg gctgatcttc
     6061 gttttcgacc gcgcaaccgg tgtgccgttg gtcccgatcg aagagcgccc cgtgccccag
     6121 gacgtcaagg tcgacggcga ggtgctgtcg ccgacccagc cgtttcccgt cggtatgccc
     6181 gcactcggac cgctgggatt ctctcccgag gacgcgtggg gattcacgcc ggtcgacaaa
     6241 tggctgtgtc gtgaaaggat caaggagctc aaccacgggc cgatctacac gcctcccagc
     6301 gagaaaggaa cgatcttttc gccctcggtc ggtggcggtc ccaactgggg tggcggcgcc
     6361 tatgacccgc agagccacat catggtggtg ccgtccaacc gcgtacccgt ggtcgtgacg
     6421 ctgatccgcc gggaaaacgc caggctcgac gagacccagg ccattgaagc cggtgccgcc
     6481 atgaccttcg ccaatgcggg ggcgccctat gttcccaagg tggagccgct tctgtcgccg
     6541 ctgggtgccc cctgctccga gcctccctgg gcagcgctga ctgcggtgga tctggccaag
     6601 ggcgagatcg tctgggacgt cccgctgggc agcatcgaga agctgatgcc gatacccatt
     6661 ccgtgggagg caggcacgcc tggtgccggg ggcccgctgc tcaccgcggg cggcctggtt
     6721 ttcatcggct tcacgctcga tgacaagctc cgcgcgttca gcctggatac gggcgaggtg
     6781 ctctggagcg ccgacctccc cgccgccggg accgcgatac ccgtcactta tgaagcgggt
     6841 ggcgagcagt atgtcgtcat tgccgccggt ggccacagca tgttcggcac cacgctgggc
     6901 gattcggtgg tggcctacaa gctcaagcgc tgatgacgtg aagcagctgc cgaccacccg
     6961 gccctagaca gccacgtcga acttcatcgg catctccttg atgccgtgga tgaagttcga
     7021 gcgcagccac ttgatctcgc cgtcgaggcg cggattgcgg atgcgctttg tcagttcctc
     7081 gaacacgata ttgagctgca accgtgccag gtggctaccg agacagaaat gctcgccgat
     7141 gccgaaggcg cgatggttgt tgcgcacgtc ctcgcgccgc gggcgcgtga tgtcgaagca
     7201 gtcgggattc tcgaacaccg cctcgtcgcg gctggcagcc tggtagaaca tcaccacctt
     7261 gtcgccggcc gcgagcgcct ggccgccgac ttccgtgggt tgcgtgacgg tgcgccggaa
     7321 attgatcacg gcggggttga agcgcaggat ctcctcgacc gcgtccggga tcagagtggg
     7381 atcctcgacc atcatccggt attgctccgg gtgttccatc agcagccgca tgccctggct
     7441 ggtcatggtg cgcgtggtct cgttgcccgc cacgatcaac agcagcatga aactgcggaa
     7501 ctcgtcctcg ttcagcttct cgccgccgac gctgccacgc agcaatgcgc cgacgatgtc
     7561 gtcctgcggg tcgtcgcgat ggtgcgccat gatcgtatcg gcgtacatgt acagctcggc
     7621 ggcggccatc tgtccgtcga gctcgctgag gttgagatcc ggatcgtcag cgccgagcat
     7681 cgtgttggtc cactcgaaga atttcgtgcg gtcctcgatc ggcacgccga ggatctcgca
     7741 gatcgtgatc agcggcagca cgcaggcgac gtcggtcacg aattcgcatt cgccgcgcgc
     7801 cacgatcctg tcgatcgcct cgcgcaccac ctcgcgaaag cgcggcgcgt aggaatccac
     7861 cttcgacggc gtgaacgcgt tgcgcacgat gcgccggtac ttgacgtgct cgggcggatc
     7921 catgttcagg atcatcgtgc gcatcagcgg cagctggtct tcgggcacgt cgttgaggaa
     7981 cgcgctcttc gccgccgagg aaaacagcgc cgggttcttc gagatgcggt cgacgtcctc
     8041 ctgcaggaag aagccccaga agccctcgcc tccgcgcatc ggcgcgtcct ggcgcaccac
     8101 gcgcgcttcg cgcagttgac ggtaaagctc ttcgggcacg ccgccgcgat ggagatcggg
     8161 atcgctgagg tcggcatagg gacacttgga catgcgggag cacctcgaaa ctaacgccta
     8221 gtagtcttgg gtcagtctgg ccccctgccc ctgcactgtc aatctcgtgg caaccatttg
     8281 cagatgacca attggtaaac cgccggcgac tgtcccgata cgcagcgctt ggcctccacg
     8341 cgggttaaac tcagccgcaa cagcgccgga gaaccccatg agatacctgc acacgatgat
     8401 ccgcgtcgcc agcctggacg acgcgctgga tttttactgc cgcaagctgg gactggcgca
     8461 gcagcgccgc tacgacaacg aggcgggccg cttcaccctg gtctacctcg cagcgccgca
     8521 ggatgccgac gccgccgagc aactcagcgc tccgcagctg gagctgacgt ggaactggga
     8581 tccggaggtc tacaccggcg ggcgcaattt cggccacctc gcctacgagg tcgcagacat
     8641 ctacgcgttg tgccggcggc tcgccgacgc aggtgtcacg atcaaccgcc ctccgcgcga
     8701 tggccgcatg gccttcgtgc gttcccccga cgggatatcg atcgagttcc tgcagcgcgg
     8761 cgcggccctc ccgccgcagg agccgtggct gtccatgccc aatacgggca gctggtgagc
     8821 gactacagca ccacccgcga ccgccggatc gccggcgccg cggtctgggc cgccacggtc
     8881 gcctcctcca ggagcaagga acgctggcat ccggggcgcc gatgcgggtt acactagttg
     8941 caataccgcg ctcgaggaag cgaccacgat ggcttactgg tactggctgg cattcggcat
     9001 ggcactcatg gccgtggaga tcttcctgcc gagtttcacc gcgctgtggt tcggcgtcgg
     9061 ggccatcatc gtcggcgtgc tgctgctcgc acttcccggc atgacgctcg cgtggcaggt
     9121 gctgatctgg gcgctcgcct ctgccgcgtt caccttcgcg tggttccggt tctttcgcaa
     9181 caaggcgccg gatcgcacca aggcgggcat gggacgcgag gcattgctcg gtgagaccgc
     9241 gatggtcgtc cacgcgccgc tcggcgagaa acgcggcctg ttgcgcttcg ccacgccgaa
     9301 gctcggttcc gacgaatggc agttcatctg caaccaatcc gttgccgagg gcgatcgggt
     9361 ggtggtcgac gatgtgtcgg gcaacacgct gatcgtcagc aagcgctgag caattccgca
     9421 tcaggttcca tccagggagt catcatggaa agtctgtttc ccgtactggt tttcttcgcc
     9481 ctcgttttcg ttacgatcgc caagctcgcc tgcatcgtgc cgcagggaag caaatgggtg
     9541 gtgcagcgcc tcggcaagta ccacaagacg ctcaatccgg gcctgagttt catcatcccg
     9601 ttcttcgacg tggtggccta ccgggtgacg accaaggaca tcgtgctcga catcccgtcg
     9661 caggaagtca tcacgcgcga caacgtcgtg atcatcgtca acgcggtggc ctacatcaac
     9721 atcctgcagc cagagaaatc ggtctatggc gtcgaggatt tccgcatcgc gatccagaac
     9781 ctggtgcaga cctcgctgcg ctcgatcatc ggcgaaatgg acctcgacga cgcgctgtcc
     9841 tcgcgcgacc agatcaaggc acgcctgaag gaagccatct ccgatgacat ctccgactgg
     9901 ggcatcacgc tgaagaccgt cgagatccag gacatcaacc cgtcggaccg gatgcaggcg
     9961 gcaatggaag aacaggcggc agccgagcgc cagcgccgcg ccacggtgac gcgcgccgag
    10021 ggcagcaagt cggcggcgat cctcgaggcc gacgggcgcc tcgaagcctc gcgtcgcgac
    10081 gcggaagcgc aggtggtgct ggccgaggcg agccagcgcg ccatcgaaat ggtcacgacc
    10141 gcggtgcagg acaaggaatt gccggtgatg tacctgctcg gcgagcgcta catccagtcg
    10201 gtgaaggaca tggccgtctc gccgaacgcc aagctggtac tcatccccgc cgacctgccc
    10261 gcctcgatcc gcggcatgat cggcaagtag ctagaccggc cggctcaggc ggttctcgcc
    10321 ggcgcgcacc gtttcgcggt gcgcgatcgc cacacggctc tgcggatcgg tgggcgcggc
    10381 cacgaaatgc cagctcgcct ccacccgctg cgcgttcacc tccaggctga cgaaaccgcg
    10441 gttcgtgtag tccgcgaaac gcaggtgcgg ctgctgtttt atgccctcga ccatgcgggc
    10501 gtgggcctcg gggtcgcggc gcgccaggta gtggccgagc ggctccgagg cgatgcccgg
    10561 ggtcacgaac tccaccgcct gcgcaccgcg ccccgattgc ccgtcgtaac gtgcgcgatc
    10621 gtagggatcg ggggcgacgt ccagtgccca ggagctgtgg atgtccccgg tcaggaacac
    10681 cacgttgtcg atcccggcgc ggcccaggtg ttggaggatg cgctggcgcg cctgcggata
    10741 gccgtcccat tggtcggggt tgaagttgcc gagcggcgcg aacacgacct gttgcccgag
    10801 caaacgccag cgcgtgccgc gggcctgcga ggcatccagt tcgccgagta gccaggcttc
    10861 ctgcgctgct cccagcagct cgcgtgccgg atttgacgcg atatcctgcg gcgatgccac
    10921 cggctgctcg cgcccctcga gccgcgtgtc gagcatcacc aggtccgcga gatcgccgaa
    10981 acggaagctg cgatggatcg gcgcgtcggc tgcggtatcg cgcaccggca tccattcgtg
    11041 ccacgcctgt attgccgcgg cgcggcgctc gcgccaggga ccttcgcgtt ccgggtcgtg
    11101 gttgccggcg ccgtcgcgcc aggcattgtt cgcgctctcg tgatcgtccc ataccgcgat
    11161 gaacggatgc gcgcgatggc aggcttgcag ggcggggtcg cgccggtaaa acgcgtagcg
    11221 gcgacggtaa tcctcgagcg tcaggatttc gtgcggcgga tcgagcggca ggcgcccgtc
    11281 ggtctggtcg ccgtactcgt agatgtagtc accgaggtgc agcaccgcgt cgagcgattc
    11341 cagttcggcg atgcgcgcgt acgcggcgaa gctgccctgc tggtagttcg cgcaggccgc
    11401 gaccgcgaaa cgcaggttct cgaccgcatc ctgcggcagc gtgcgcgtgc gccccaccac
    11461 cgagtgctcg ccgtggacgc gaaacgcgta gaagtacgtg gtcccgggtt ccagcccgcc
    11521 cacatcgacc ttgcagcaga aatcgcgcag cggatccgcc acgacgcgcc cgcgccgcac
    11581 gacatgacgc aattgcggct cgcgggcgac cagccactcg agctcctcgg ccgccccggc
    11641 accatcgaga cgcgtccaga tcacgacgcg ctccgctccc ggatcgccgc tggccacgcc
    11701 gtgcgcgaag cgcggctggc cgcgatcgcc ctgcgtcccg gcgcagccac tggcgccgag
    11761 cgcggccgca aggccggcgc cggcgaggaa gccgcggcga tcgagcggtg gcatcaggcc
    11821 accgtgccgg cggcacgcat ccgggcgatc tgttccgcgc tgaagccggc ctcggcgagc
    11881 aggacatcgg tgtcggcccc gatcgccacc gcgggcgcag gacgctcggg cgcggagcgg
    11941 ctgaagcgcg gcgcgggcgc gggctgccac acgccgtcgt cctcgatgaa cgtgccgcgc
    12001 gccttgatgt gcggatggct ggtgacctcg tcgaagccga gcaccggcgc gaagcagata
    12061 tcggtgccct ccatgatgcg gcaccattcg tcacgcgtgc ggctgcggaa gatttccgcg
    12121 aagcgctgct tcaaggcgtc ccagtggcgc gcgtcgtgct ggtgcggcag ttcctcgccc
    12181 gccagcccga gcttctcgag cagcagcgcg tagaactgcg gttcgatcga gccgatcgag
    12241 acgtacctgc cgtcggcggt ctcgtagaca ccgtagaagt gggcgccgcc gtcgagcatg
    12301 ttgctgccac gggtgttgtg ccagaagccc gactggaacg ccccgtacat catggtcatc
    12361 agcgccgagg cgccgtcgac catcgccgca tcgaccacct gccccttgcc cgaggcctgc
    12421 gcctcgagca tcgcgcacac gacgccgaag gcgagcagca tcccgccgcc gccgaaatca
    12481 ccgatcaggt tgagcggcac cacgggcttc tggcccttct cgccgattgc gtgcagcgcc
    12541 ccggtgagtg cgatgtagtt gaggtcgtgg ccggcggcgt tgccgagcgg gccgtcctgg
    12601 ccccagccgg tcatgcgccc gaacacgatg cgcgggttgc gcgcgaggca gacctcggga
    12661 ccgagcccga gtttctccat cacgccgggg cgataaccct cgatgatcgc gtcggcggat
    12721 tcgaccagac gcagcaccac gtccgcggca tgcggatcct tcaggttcag cgcgatgcag
    12781 cgcttgccgc ggttgccgaa atcgagtttc tcgttctgcg ccatgctcag catggaaccc
    12841 gaggggcgcg aaacgcggat cacctcggca cccatgtcgg ccagcatcat ggcggcaaac
    12901 ggcgccgggc cgatgcccgc gatctcgaca atcttcttcc cgctcaatgg acccatcgca
    12961 aacctgcctg cttcgtggaa aaacagcggc ggagtctacc gcaggcggcg ccagtgcgac
    13021 cacttgtgcc gccgcgcacc cccgcgaggt cagccctcgc cgccggatcc cttgatcagg
    13081 caatgcttcg cgtaactcgc cgcctcgtca gtgctccatt cgcccttggg cttgtctgcc
    13141 agcttgcgac accagcgctc gccgccgacc tcggtcgcct gcagcaggca atgcttcgcg
    13201 tagtccttcg cttcgcccag gctccagtcg gtcttgggct tctgcgccag tgcctcgcac
    13261 cagcgcgcgc tgcccacctc gggctcgcac gcggcggcga gtgccgcgag caggatcagg
    13321 gcgcccgtcc tgatcgtggt gttcatcctc atggatcatc tccctgtggc tcttgcgttg
    13381 attcccgcgc gacgaacacg tctcccacgc ccagcgatgt gccctcgccg ccctgccagg
    13441 tggcgttgat gccgaacaac ggctttgccg tgccgggcag cgggttcatc gcggcgagca
    13501 tcgcaaacgc ggccctgccg ggttcgtcgc gcacgccgag cgccggatcg accgtgatca
    13561 ccgcgcagcg ttcgcagggg tagcatagcc gaaacgatcc ctcgtcccgc tgcaaggcag
    13621 cgagtgcgtg ctcgcgaaac gcatccaggc cttcgaccac gatattggga cggaagcgtt
    13681 ccatacccac cgcgggttgg ccgcgttgcg cgagcgccgc gttgagggcc cgcagcgagg
    13741 ccatattcac gaccaggaac ggcgcggcat cggcgaaggc ggtcgtcgtc gcatcgccca
    13801 gcagcggcgc acgcgccagg cgacggcgga atccgcggcg catgcgcacg agccgcagcg
    13861 ctcgctccgg ctgcaccgcc gcctgcaacc agcgcgaggc ctcggacccg gcatcgagca
    13921 cctcgcacgc gtcggaccac acctgcgccg tgaaggactc ggcggcgcgg tatgccagcg
    13981 gcacgcgcag cggatcccgg ccaggagcct cgatgcagag cgagtcgtcc tcgtagcgcg
    14041 gcgcgagcag cgccaggcgc ggcagctcgc gctgcg
//
LOCUS       DAORUQ010000080        13957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4242_length_13957_cov_5.472810, whole genome shotgun sequence.
ACCESSION   DAORUQ010000080 DAORUQ010000000
VERSION     DAORUQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_13957_cov_5.472810"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLW13_08605"
     CDS             complement(<1..1216)
                     /locus_tag="PLW13_08605"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70474.1"
                     /translation="MEYDLVIRNGRIVDGTGAAAFTGDVAVQGGRIACVGRVAAQGRR
                     EIDAAGKLVTPGWVDVHTHLDGQATWDSLLMPASNHGVTTVVMGNCGVGFAPCQPTSE
                     AHDALIAVMEDVEDIPGTALHEGISWTWESFPQYLDTLERMPHAIDVAAQVAHCAVRT
                     YVMGERGARNEPATPEDIARMAAIVREGLEAGAIGFSTSRTQLHRTRDGEVMPGTYAD
                     EAELLGIGKVLGETGKGVYQLVSDWDEWEKEMDWMKRLSISTGRPVGFVLFYRADEEW
                     ERVQRQLEYVRQANAEGAQLVPHVGARPVTILMAIDGTAHPFMFHQNFQPLAALPAAE
                     RLAKLRDPAVRAAILAETPPVLGHPIADRLAVEFDNMYPLEDDPSYEPRAEDSIAAQA
                     ARLGVSPAELAYD"
     gene            complement(1461..3713)
                     /locus_tag="PLW13_08610"
     CDS             complement(1461..3713)
                     /locus_tag="PLW13_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70475.1"
                     /translation="MHIRKKTISQAIGLAALLVAGAQAQAQTGGLEEMVVTAQKREQS
                     LQQAPIAISAFGRDALQQQRITDVQDLNGLVPNVQFAASPSSTTGSTVAIRGAVTVNP
                     APTWEPTVGMYVDGVFIGKVLGGIFDVADLERVEVLRGPQGSLYGKNTVGGAVNLITR
                     KPSGELMGEVKAGVGNEGYWQMRGSLDTPAIGTVGEGLGQLSANITLQHEERDGFTRG
                     VADPVGSPLAAPAVADEWDGLDSDSGRVALLLNVTEEFEARYTYDFSDKQNTPQAPHL
                     THADPNVVPFLLPYLVSEDERSDKLSSDTDVYEESDIEGHALHLGYDLGDMGFLGNVE
                     LRSITAYREMEWDDLLDIDGSPIDAFHSGRGIDYDQTSQELQIVGSTDRVNYVLGYYY
                     FDETADVTNPITFFGVFGAPTAPNAYGLESESNAVFGQLDWKPGAAALQDRLTVTVGA
                     RYTEEEKDQYISHPIVTDANLQVQPDWAFAGQADEDWNKFTPSLTLSWDLTEEASIYA
                     RYAEGWKSGGFNGEASTLESFQRAYDPEEVASYELGLKSRWLDNRVQVNAAAFENQVD
                     DMQIAVFLADGSAASVVDNAGQATVRGFELEVLAEPVDDLMLNFSWGYLDPEYDEYLD
                     GGVDVSDDRDFPYAPENTLTAGVQYTVAGVLGGNLIGRLDWSYTDDRVLYPEPAQNLH
                     SQFDDYALLNGRLSLTELPLGNGSLELAAWGKNLTDEDYRVSSIPFLIWTASYFGAPR
                     TYGMEVSYNF"
     gene            4026..4472
                     /locus_tag="PLW13_08615"
     CDS             4026..4472
                     /locus_tag="PLW13_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70476.1"
                     /translation="MSLEMLLAEREIYRALVRFARAMDQRDWHALEAIVRPDATADLG
                     LGTAHGREAIVGVMRSFLDDCGPTQHLLGNVTIDIDGDTARSSCYVSDMHKGTGAKAA
                     LTFSTLGEYHDRWQREGDAWRMTHRTKLNRAHIGDIGVLGPGPKRS"
     gene            4501..>4907
                     /locus_tag="PLW13_08620"
     CDS             4501..>4907
                     /locus_tag="PLW13_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70477.1"
                     /translation="MTVAQTVRCDEAAPGFRPARGLGNAHLQSFLASTGPRRALTSRR
                     AHALQAAAREELLDCGEGIRLHGVYSPHPRAERGLAILIHGWHGSADSAYLLSAATHL
                     FARGFSVFRLHLRDHGPSHHLNRELFNSARLPEV"
     assembly_gap    4908..5007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5100..6263
                     /locus_tag="PLW13_08625"
     CDS             5100..6263
                     /locus_tag="PLW13_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70478.1"
                     /translation="MKFLKRALLLVAILAGVLALAWKLTEPAPLPPGSAGAALLAQPP
                     WGVGMREFELLDSSRPTGANGDFPGAPERRLRARAWYPLDTDGRSIPAYAFPLIVYSH
                     GFFSSSAEPAYLARHLASHGYVVVAADFPLTTMKSPGGANVFDVVNQPGDVSFLIDTL
                     LAWNGNPANDFHQRIDATRIGIAGTSLGGMTTELTAYHPRLRDPRVKAAVSIAGPLQL
                     FGRAFFTDSTLPFMMVAGDIDAMVPYEQNARPVLERVNNAWLVSLAGASHTGFADQGS
                     VFRWMANPDSVGCYFVRGRTPQASEDTSFYDRLGTVEEGVLRGMEDSLCRMDPLPDAM
                     SPLLQQRITMLAVRSFFESVFNPDASQQARYRDYLQAGLARDFPAVTVERSTQ"
     gene            complement(6321..7235)
                     /locus_tag="PLW13_08630"
     CDS             complement(6321..7235)
                     /locus_tag="PLW13_08630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70479.1"
                     /translation="MRIGLMMGASREIGEDLPGIVAFAQQAEAMGFAHVWLAHTMGVD
                     AMTALALTGAQTTRIRMGTAVVPTFPRHPVAMAQQALTVAAASGGRFELGIGLSHKML
                     IEDMYGIPYAHPAAHMREYLEVLLPALRGEQLQHKGERFSVNMRLAVPGATAVPVLVA
                     ALGPVMLRIAGELAEGTITWMTGPRTLAQHIVPTLHRAAAAAGRPAPRVVAGMPIVLT
                     ADADAARRKISELLAIYGMLPSYRAMLDREGAAAPGDIAMVGDEAALRAQLARLRDAG
                     VTDFDAAIIALDDGAVARTRDFLATELP"
     gene            complement(7265..9292)
                     /locus_tag="PLW13_08635"
     CDS             complement(7265..9292)
                     /locus_tag="PLW13_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQY70480.1"
                     /translation="MNGTTNLRRAIIAGMSLLALPAHAELPFDQLIVFGASYEDVGQF
                     PDYDFVAEAFPSLVAAPGAGLDGSTGLRVTNIDPATGRRGQVWVEMLADRIGTGPLVP
                     STPLLYPGARSDIPDTDNIDFAFVTARSAELLEAVTGTSRVAHPTDALVAADLTASSP
                     GFLQRLQAGTLKIGPDTLFVLNPAGNDVRDSAVDDPTATATVGAANTLEIMRQLVGAG
                     ARTIVVPTFAPLGLLAESTNVNPDGSRTAKAEARGIGAATYNALMAEGLPGVGGNIVV
                     VDFDTLFREVLAAPAAFGFNAGIDQSRYCYSASEWSLTGIDCTEAPGLGKSSGGTPKD
                     FVTSDGLHPTGGMTRILADYTESVLRAPGFAALLPEAVLADARGYLNTVQDQLAQNRW
                     GDRAFEPQLFVAVQGRRLDMADTRARPEASSDAADLTIGGSLGLGGGWFAGGALGSQQ
                     GDADIEHSSFEGSGLMGSLFAGYRCEGWFADAVLSAGETDLDDIERVFEVGTWQLRRE
                     SGDTRADVLGVSGTIGVNMMGEESVWRFGPFLAADYLDIEVDGYQEKSANSTAMAFGD
                     LERDSVTGSAGVFASYPFRIGAADLELRGDAAWVEEFEDRSDEVRAVVKRVADGVWFR
                     MPGYDIDDAGLRAVLGVDAGWRSGLRLGLSYRYADNEAEAQYLNLSASYAF"
     gene            complement(9405..11327)
                     /locus_tag="PLW13_08640"
     CDS             complement(9405..11327)
                     /locus_tag="PLW13_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQY70481.1"
                     /translation="MRGLIRTAIAAASLASAAAWAGVPFGQFVAFGASYDDSGQFPDL
                     ELGGTTGLRFTNIDPATGLRGLSMPEWLALDLGLGRMNPSTPLLVFGARTDTVATDNL
                     NFAVGGYRSEEVLASVTGNSVVSAGGFRVEQPGFLARVASGALTVGPDTLYYALIAGN
                     DIRDVDDPRQTADVSAAIAQALVDAGARYIVLPTLPKLGNFSEAVNLSADGRTQLAAD
                     RTAAAIAYNAAFAERLNTIDGNFIRIDVQRLFDEVLADPVGFGYPADLDQARVCYSAG
                     EAGGIDCVEPAGRGKASGGDPDQFVFQDGLHPTQAAARAAADLMESVIRAPGMVALLP
                     EAVLADTRGYLRTVEEHLAQNRWAQGAQAAQLFVAVQGRDIDIADAWSTPAASSDAVD
                     LTLGASVGVGERWLLGAALGSQQGETDVDGGGFDSAGLTGSLFAGYRCGAWFADAVLS
                     AGRSELDDIRRVFDVGSVQRRRESGDTDADLLGLQATLGVNLLGAAAAWRAGPFVALD
                     ALQIDVDAYAERGLSSSTMRFDDIARDSLLGTAGVFASYPLRLGAAEVELAADVGYAG
                     EFEDESDEVRAVVKSLDAGPWFRMPGYAIDDDGWRATLGANASWPGGMRLGLSYRHTD
                     NDVETRALNLSAAYRF"
     gene            11499..13049
                     /locus_tag="PLW13_08645"
     CDS             11499..13049
                     /locus_tag="PLW13_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HQY70482.1"
                     /translation="MRSPTACTLASLLFAQRALAEAPRGIDQQIAQGFKPFADAVSGF
                     VFAAIPFGDHAVPWILFWLIVAGIFCTLYFRFINLRGMAQGMRIIRGDYDAPHHHGDT
                     SHFQALATALSGTVGLGNIAGVAVAVTVGGPGAAFWMIVAALLGMATKFCECTLAVKF
                     RHRRPDGTVSGGPMYYLAQGIAAEYPRLAPLGRLLGVSFAVFCVFGTLGSGNFFQVNQ
                     AYHQLLLITGGDASVLAGRAWMFGIAMAVATGIVVLGGIQRIAKVTDKLVPLMALLYL
                     GAGLIVIAHHVDLLPQALAAIVNGAFSAEGVAGGVLGVILQGFRRAAFSNEAGIGSAP
                     IAHASVRTDEPLTEGLVALWEPFIDTVIICTMTALVITISGVSAASTGAQGVALTSAA
                     FATVMPWFPYALALVVLLFAYSTMIAYCYYGTKAANYLFGESAAVDVCYKLFFLGMTV
                     FGVTMDLSQLVDFADAIYFLMAVPNIIGLYLLAPVVKREMDSYFARLARGEIRPGREA
                     PEHLLGSP"
     gene            complement(13051..13752)
                     /locus_tag="PLW13_08650"
     CDS             complement(13051..13752)
                     /locus_tag="PLW13_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQY70483.1"
                     /translation="MGAQWKAKGRADAAATKGRIFTKLAKEIMVAARTGADPDSNPRL
                     RKAVEQAKRASMPRDTLERAIKKGAGLLDEHVQYESLTFEGFAPHQVPVIVECLTDNK
                     NRAAMSIRMLFRKGQLGNSGAVSWDFKYLGMIEATPNAPDADPELAAIEAGAQDLEPL
                     EDNVTLFLTDPTDLDAVTRALPGFGFTVQSAVLGYRPNNPVQLDAAARAEVEAFLEAL
                     DADDDVQNVYAALAD"
BASE COUNT         2139 a         4870 c         4622 g         2226 t
ORIGIN      
        1 ggtcgtaggc gagctcggcc ggactgacgc ccaggcgcgc ggcctgcgcc gcgatgctgt
       61 cctcggcgcg cggctcgtag gacggatcgt cctcgagcgg gtacatgttg tcgaactcca
      121 ccgcgagccg gtcggcgatg ggatggccga gcacgggcgg cgtctcggca aggatagcgg
      181 cccgcacggc cggatcgcgc agtttcgcca gccgctcggc ggccggcagc gcggcgagcg
      241 gctggaagtt ctggtggaac atgaacgggt gcgcggtgcc gtcgatggcc atcaggatgg
      301 tcaccgggcg cgcacccacg tgcggcacca gctgcgcgcc ctcggcattg gcctggcgca
      361 cgtactcgag ctggcgctgc acgcgctccc actcctcgtc ggcgcggtag aacagcacga
      421 agcccaccgg gcggccggta ctgatcgaca gccgcttcat ccagtccatt tccttctccc
      481 actcgtccca gtcggacacg agctggtaga cgcccttgcc ggtctcgccc agcaccttgc
      541 cgatgccgag cagttccgcc tcgtcggcat aggtgccggg catcacctcg ccgtcgcggg
      601 tgcggtgcag ctgggtgcgc gaggtgctga agccgatcgc gccggcctcg agtccttcgc
      661 gcacgatcgc ggccatgcgc gcgatgtcct cgggcgtggc cggctcgttg cgcgcgccac
      721 gctcacccat cacgtaggtg cgcaccgcgc agtgcgcgac ctgcgcggcg acgtcgatcg
      781 cgtgcggcat gcgctccagg gtgtcgaggt attgcgggaa gctctcccag gtccacgaaa
      841 tgccttcgtg caacgcggtg ccggggatgt cctccacgtc ctccatcacg gcgatcagcg
      901 catcgtgcgc ctcggacgtg ggctggcagg gcgcgaaacc cacgccgcaa ttgcccatca
      961 ccaccgtggt gacgccgtga ttggacgcgg gcatcagcag ggaatcccag gtcgcctgcc
     1021 cgtcgaggtg cgtgtgcaca tcgacccagc cgggcgtgac cagcttgccg gcggcatcga
     1081 tctcgcgccg gccctgagcg gcgacgcgac cgacgcaggc gatgcgtccg ccctgcaccg
     1141 cgacatcgcc cgtgaacgct gccgctcccg tgccatcgac gatgcggccg ttgcggatca
     1201 ccaggtcgta ttccatggtc ctgccctttt gacaatgtat tgctgtggat cggcattcgt
     1261 gtgggtcggc attcacgccg acttgtgcct gcccgcacgc ggcggcttcg gctgcgccga
     1321 aaccttgtcg ggatgaatcc cgacctacaa gccgatgcga gtcaatccca gggcgggggg
     1381 tggagaaatt aagctttgtt aaacgcaaac gggcgtggat ctctccacgc ccgttgcggt
     1441 tcggaccgaa ggtgcggaaa tcagaagttg tagctgactt ccatgccgta ggtccgcggc
     1501 gcgccgaagt agctcgccgt ccagatcagg aacgggatcg agctgacccg gtagtcctcg
     1561 tccgtgaggt tcttgcccca ggccgcgagt tccaggctgc cgttgccgag cggcagctcg
     1621 gtgagcgaca ggcggccgtt cagcagcgcg taatcgtcga actgcgaatg caggttctgc
     1681 gccggctcgg gatagagcac gcggtcgtcc gtgtagctcc agtccagacg accgatcagg
     1741 ttgccgccga gcacgccggc caccgtgtac tgcacgccgg cagtgagggt gttctcgggc
     1801 gcatacggga agtcgcggtc gtccgacacg tccacgccgc cgtccaggta ctcgtcgtat
     1861 tccggatcga ggtaacccca gctgaagttc agcatcaggt catcgaccgg ctcggcgaga
     1921 acctcgagct cgaagccccg cacggttgcc tggcccgcgt tgtcgaccac ggaggccgcc
     1981 gaaccgtcgg cgagaaacac ggcgatctgc atgtcgtcga cctggttctc gaaggccgcg
     2041 gcattgacct gcacgcggtt gtccagccag cgcgacttca gacccagctc gtaggatgcc
     2101 acttcctccg ggtcataggc gcgctggaag gattccagcg tcgaggcctc cccgttgaaa
     2161 ccgcccgatt tccagccctc ggcgtaacgc gcgtagatgc tggcctcctc ggtgagatcc
     2221 cagctcagcg tgagcgaagg cgtgaacttg ttccagtcct cgtcggcctg gcccgcgaag
     2281 gcccagtccg gctgcacctg caagttggcg tccgtgacta tcgggtgcga gatgtactgg
     2341 tccttctcct cctcggtgta gcgcgcgccg accgtgacgg tcaggcggtc ctgcagcgct
     2401 gccgcgccgg gtttccagtc cagctggccg aacacggcat tcgattcgct ctcgagcccg
     2461 taggcgttgg gcgcggtcgg tgcgccgaag acgccgaaga aggtgatcgg gttggtgacg
     2521 tcggcggttt cgtcgaagta gtaatagccg agcacgtagt tcacgcggtc ggtcgaaccg
     2581 acgatctgca gttcctgcga ggtctggtcg tagtcgatgc cacggcccga gtggaaggcg
     2641 tcgatcggcg agccgtcgat gtcgagcagg tcgtcccatt ccatttcgcg ataggccgtg
     2701 atcgatctca gctccacgtt gcccaggaag cccatgtcgc ccaggtcata gcccaggtgc
     2761 aacgcatggc cctcgatgtc cgattcctca tagacatcgg tgtcgctgct cagcttgtcc
     2821 gaacgctcgt cttcggaaac caggtagggc agcaggaagg gcaccacgtt gggatcggcg
     2881 tgcgtcaggt gaggtgcctg cggggtgttc tgcttgtcgc tgaagtcgta ggtgtaacgc
     2941 gcttcgaact cctcggtgac gttcagcagc agcgccacgc ggccggagtc ggaatccagg
     3001 ccgtcccact cgtcggcgac ggccggggcc gcgagcgggc tgcccacggg gtcggcgacg
     3061 ccgcgcgtga agccgtcacg ctcctcgtgc tgcagcgtga tgttggcgct gagctggccc
     3121 aggccttccc ccacggtgcc gatcgccggg gtgtcgaggc tgccgcgcat ctgccagtag
     3181 ccctcgttgc cgacgccggc cttcacctcg cccatcagct cgcccgaagg cttgcgggtg
     3241 atcaggttca cggcgccgcc gacggtgttc ttgccgtaca gggagccctg cggaccgcgc
     3301 agcacctcga cgcgctccag gtccgccacg tcaaagatgc cgccgagcac cttgccgatg
     3361 aacacgccgt ccacgtacat gcccacggtc ggctcccagg tgggtgccgg gttgaccgtg
     3421 accgcgccgc ggatcgccac ggtggaaccg gtggtgctgc tcggggacgc cgcaaactgc
     3481 acgttgggca ccaggccgtt cagatcctgc acgtcggtga tgcgctgctg ctggagcgca
     3541 tccctgccga aggccgagat agcgatgggg gcctgttgca gcgactgctc gcgcttttgc
     3601 gcggtcacca ccatttcttc cagcccgccg gtctgggcct gggcctgggc gccggcaacg
     3661 agcagcgcgg cgaggcctat ggcctgcgaa attgttttct tgcgaatatg catcatctgt
     3721 tcccctgaat tgacacttat atcgagccac ccttggcgaa gcagggtttg gccggccgga
     3781 gtctagacac gcaagggctg tcggcatccc tcaaacgaag tattacggca cccggtgcgc
     3841 aaaagttaca cgaatcgacc cgcgcgagcc tgaaccagcc tgccggcacg acgcacaccc
     3901 ccgcagaaac atgcgtattt tctaatcaat ggctaccggt aacgcgccct gtacaatccg
     3961 gccttgccgc cgcggccttc gccggacgaa catggcaggc cacacgaccc gacgggaatg
     4021 tacgcatgag cctcgagatg ctgctcgccg aacgcgagat ctaccgcgcc ctggtgcgct
     4081 tcgcccgcgc catggaccag cgcgactggc acgcgctgga ggcgatcgtg cgccccgacg
     4141 ccacggccga cctgggcctg ggcacggcgc acggccgcga ggcgatcgtg ggcgtgatgc
     4201 gctccttcct cgacgactgc ggtccgacgc agcacctgct gggcaacgtg acgatcgaca
     4261 tcgacggcga taccgcgcgc agcagctgct acgtgagcga catgcacaag ggcaccggcg
     4321 ccaaggccgc gctcaccttc tcgacgctcg gcgaatacca cgacaggtgg cagcgcgagg
     4381 gcgacgcgtg gcgcatgacg caccgcacca agttgaaccg cgcgcacatc ggcgacatcg
     4441 gcgtgctcgg gccgggaccg aagcgctcgt gagaccggct gctccaatcg cgccgcaccc
     4501 atgactgtcg cgcagaccgt acgctgcgac gaggcggcac cgggcttccg gccggcgcgg
     4561 gggctcggca acgcgcacct gcagtccttc ctcgcgagca ccggtccgcg ccgtgcgctg
     4621 acctcgcgcc gggcacacgc gctgcaggcc gccgcgcgcg aggaactcct cgactgcggc
     4681 gagggcatcc gcctgcacgg cgtgtattcc ccgcatccac gcgcggagcg cggcctcgcg
     4741 atactgatcc acggctggca cggcagcgcg gactcggcgt acctgctgag cgccgcgacg
     4801 cacctgttcg cgcgcggctt ctcggtgttc cgcctgcacc tgcgcgatca cggcccgagc
     4861 catcacctga accgcgagct gttcaactcg gcgcgcctgc ccgaggtnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc ggcgggcact gtggcttcat catggactgg
     5041 cgcctgcaca gctggatcga cgggcgcctg gatcaactgt tccgtcccgg gagactgccg
     5101 tgaaatttct caaacgcgcc ctgttgctcg tcgcgatcct cgccggcgtg ctcgcactgg
     5161 catggaagct gaccgaaccc gcaccgttgc cgcccggcag cgccggcgcc gccttgctgg
     5221 cccaaccgcc gtggggcgtg ggcatgcggg aattcgaact gctggacagc agccgcccga
     5281 ccggcgccaa cggcgatttc cccggcgcgc ccgaacgcag gctgcgcgcg cgtgcgtggt
     5341 atccgctgga cacggacggg cgcagcatac cggcgtacgc atttccgctg atcgtctaca
     5401 gccacggctt cttcagcagc agcgccgagc cggcctatct cgccaggcac ctcgccagcc
     5461 atggctacgt ggtcgtcgcg gccgatttcc cgctcaccac catgaagtcg ccgggagggg
     5521 cgaacgtttt cgacgtggtc aaccagccgg gcgacgtgag cttcctcatc gacacgctgc
     5581 tggcgtggaa cggcaatccc gcgaacgact ttcaccaacg catcgatgcc acgcgcatcg
     5641 gcatcgcggg cacatcgctc ggcggcatga cgaccgaact caccgcctat catccgcgcc
     5701 tgcgcgaccc gcgcgtgaag gcggccgtat cgatcgccgg ccccctgcag ttgttcggcc
     5761 gcgcgttctt caccgactcc acgctgccgt tcatgatggt cgcgggcgac atcgacgcga
     5821 tggtgcccta cgagcagaat gcgcgccccg tgctcgagcg ggtgaacaac gcctggctgg
     5881 tgagcctggc gggtgcctcc cacacgggct tcgccgacca gggctcggtg ttccgctgga
     5941 tggccaaccc cgacagcgtc ggctgttact tcgtccgtgg caggacgccg caggcaagcg
     6001 aagacaccag tttctacgac cgcctcggta ccgtggagga aggggtgcta cgcggcatgg
     6061 aggacagcct gtgtcgcatg gacccgctgc ccgatgcgat gtccccgttg ctgcagcagc
     6121 gcatcacgat gctcgcggtg cgcagcttct tcgagagcgt gttcaatccc gatgcttcgc
     6181 agcaggcgcg ctaccgcgac tacctgcagg ccggcctggc gcgcgatttt ccggcggtca
     6241 ccgtcgagcg ctcgacccag taggtccgga tttatccgga catcgcgccg caggagacag
     6301 ttgtccggat aaatccggac ctacgggagt tcggtggcga ggaaatcgcg cgtgcgcgcc
     6361 accgcgccat cgtccagcgc gatgatcgcg gcgtcgaaat cggtgacacc cgcatcgcgc
     6421 aggcgcgcga gttgcgcgcg cagcgcggcc tcgtcaccca ccatcgcgat atcacccggc
     6481 gccgcggcgc cttcgcgatc gagcatcgcg cggtaggacg ggagcatgcc gtagatcgcg
     6541 agcaattcgg agatcttcct gcgcgccgcg tccgcatcgg cggtgagcac gatcggcatg
     6601 cccgccacca cgcgcggcgc ggggcgtccg gcggccgcgg cggcgcgatg cagcgtcggc
     6661 acgatgtgct gcgccagcgt gcgcggcccg gtcatccagg taatggtgcc ctcggcgagc
     6721 tcgccggcga ttcgcagcat caccggaccg agcgcggcca ccagcaccgg cacggcggtg
     6781 gcgccgggca ccgccaggcg catgttcacg ctgaagcgct cgcccttgtg ctgcagctgt
     6841 tcgccgcgca gcgccggcag cagcacctcg aggtactcgc gcatgtgcgc cgccggatgc
     6901 gcgtagggaa tgccgtacat atcctcgatc agcatcttgt gcgagagccc gatgccgagc
     6961 tcgaaacgcc cgccgctggc ggcagcgacc gtcaaagcct gctgcgccat cgccaccggg
     7021 tgacgcggga acgtgggcac cacggcggtg cccatgcgga tgcgcgtcgt ctgcgcaccg
     7081 gtgagagcca gcgccgtcat cgcatccacg cccatcgtgt gcgccagcca cacgtgggcg
     7141 aagcccatcg cctcggcctg ctgcgcgaac gcgacgatcc cgggaaggtc ctcaccgatc
     7201 tcgcgcgagg cgcccatcat cagtccgatt cgcataacgt gtgctcctgt gggtgttttc
     7261 aagctcagaa cgcgtagctg gccgagaggt tcaggtactg cgcctcggcc tcgttgtcgg
     7321 cgtagcggta cgacagcccg agacgcaggc cgctgcgcca gccggcatcc acgccgagca
     7381 cggcgcgcag gcccgcgtcg tcgatgtcgt aaccgggcat gcgaaaccac acgccgtcgg
     7441 ccacgcgctt cacgacggcg cgcacctcgt cgctgcgatc ctcgaactcc tccacccacg
     7501 ccgcgtcccc gcgcaactcg aggtctgccg ccccgatccg gaacggatag ctcgcgaaca
     7561 cgccggcgct gcccgtgacg gaatcgcgtt ccagatcgcc gaaggccatc gcggtgctgt
     7621 ttgcggattt ttcctggtag ccatcgacct cgatgtcgag gtaatcggcc gcgaggaacg
     7681 ggccgaagcg ccacaccgac tcctcgccca tcatgttcac gccgatcgtg cccgaaacgc
     7741 ccagcacgtc ggcccgggtg tcgccggatt cgcggcgcaa ctgccaggtg cccacctcga
     7801 acacgcgctc gatatcgtcg agatccgtct cgccggcgct cagcaccgcg tctgcgaacc
     7861 agccctcgca gcgatagccc gcgaacagcg agcccatcag ccccgagcct tcgaagctgc
     7921 tgtgctcgat atcggcatcg ccctgctgcg agccgagtgc gccgccggcg aaccagccgc
     7981 cgccgaggcc gaggctgccg ccgatcgtga ggtcggcagc atccgagctc gcctcgggcc
     8041 tggcgcgggt gtcggccatg tcgaggcggc gcccctgcac cgccacgaac agctgcggtt
     8101 cgaaggcgcg atcgccccag cggttctgcg ccagctgatc ctgcaccgtg ttgagatagc
     8161 cgcgcgcatc ggccagcacc gcctcgggca gcagcgccgc gaaaccgggc gcgcgcagca
     8221 cggactcggt gtagtcggcg aggatgcgcg tcatcccccc ggtggggtgc agaccgtcgc
     8281 tggtgacgaa gtccttcggc gtcccgccgc tcgatttgcc cagccccggc gcctcggtgc
     8341 agtcgatgcc ggtcagcgac cactcgctgg ccgaatagca gtagcggctc tggtcgatgc
     8401 cggcattgaa gccgaaggcc gcgggcgcgg cgagcacctc gcgaaacagc gtgtcgaagt
     8461 cgaccaccac gatgttgccc cccacgccgg ggagtccctc ggccatcagc gcattgtagg
     8521 tggcggcacc gatgccacgt gcctcggcct tggcggtgcg gctgccgtcg ggattgacgt
     8581 tggtgctctc ggcgagcagc cccagcggcg cgaaggtggg aaccacaatg gtgcgcgcgc
     8641 cggcgccgac cagctggcgc atgatctcca gcgtgttcgc ggcacccacc gtggcggtgg
     8701 cggtcggatc gtcgaccgcg gaatcacgca cgtcgttgcc tgccgggttg agcacgaaca
     8761 gcgtgtcggg accgatcttc agcgtacccg cctgcagccg ctgcaggaaa ccggggctgc
     8821 ttgccgtgag atccgccgcc accagcgcgt cggtcgggtg cgcaacgcgc gaagtgccgg
     8881 tcaccgcctc gagcagctcc gcagagcgcg cggtgacgaa ggcgaagtcg atattgtcgg
     8941 tgtccgggat gtcgctgcgt gcccccgggt acagcagcgg ggtcgagggc accaagggac
     9001 cggtaccgat gcggtcggcg agcatctcca cccacacctg cccgcgccgg ccggtggccg
     9061 gatcgatgtt ggtcacgcgc aggccggtgc tgccgtccag gcccgcgccc ggcgcagcca
     9121 ccagcgacgg aaacgcctcc gcgacgaaat cgtaatcggg gaactgaccg acgtcctcgt
     9181 agctcgcgcc gaacacgatc aactggtcga agggcagctc cgcgtgcgcc ggcaacgcca
     9241 gcaggctcat gccggcgatg atcgcccgtc tgagattcgt ggtcccgttc atgttgcgtt
     9301 cactcctgcg ccagtcacgc aggcactgcg acagcgcccg ccgctcgtca ccttgaccgg
     9361 tgtcacgaca ccgtaacgtg tcgcgcatca acacaattgc ccgctcagaa gcggtaagcc
     9421 gccgagagat tcagggcgcg cgtctcgacg tcgttgtcgg tgtgacggta cgacagcccg
     9481 agacgcatgc cgcccggcca gctggcgttc gcgcccagcg tggcgcgcca gccgtcgtcg
     9541 tcgatcgcgt agccgggcat gcggaaccac ggccccgcat ccagcgactt caccaccgcg
     9601 cgcacctcgt cggactcgtc ctcgaattcc ccggcgtaac cgacgtccgc ggcgagctcg
     9661 acctcggccg cgccgaggcg caacggatag ctggcgaaca cgcccgcggt gccgagcagc
     9721 gaatcgcgcg cgatgtcgtc gaaacgcatc gtcgaggagc tcagcccgcg ctcggcatag
     9781 gcgtcgacgt cgatctgcag cgcatcgagc gccacgaagg gaccggcgcg ccaggccgcc
     9841 gccgcgccga gcaggttcac gcccagcgtg gcttgcagcc cgagcaggtc cgcatcggtg
     9901 tcacccgact cgcgccgccg ctgcacgctg ccgacatcga acacgcgccg gatgtcgtcg
     9961 agctcgctgc gcccggcgct cagcaccgca tcggcgaacc aggcgccgca gcggtagccg
    10021 gcgaacagcg agccggtgag gcccgcgctg tcgaagccgc caccatcgac gtctgtctcg
    10081 ccctgctgcg aacccagcgc cgcgccgagc aaccagcgct cgcccacgcc cacgctggcg
    10141 ccgagggtga ggtccaccgc atccgagctc gccgccggcg tggaccacgc atccgcgatg
    10201 tcgatgtcgc gcccctgtac cgcgacgaac agttgcgcgg cctgcgcgcc ttgcgcccag
    10261 cggttctgcg cgaggtgctc ctcgacggtg cgcaggtagc cgcgcgtgtc ggcgagcacc
    10321 gcctcgggca gcagcgcgac catgccgggc gcgcggatca cggactccat caggtcggcc
    10381 gcggcgcgcg cggcggcctg cgtcgggtgc agcccgtcct ggaacacgaa ctggtccggg
    10441 tcgccgccgc tcgccttgcc gcgtcccgcc ggctccacgc agtcgatgcc gccggcctcg
    10501 cccgcgctgt agcagacccg cgcctggtcg agatccgcgg gatagccgaa gccgaccgga
    10561 tcggccagca cctcgtcgaa caggcgctgc acgtcgatgc ggatgaagtt gccgtcgatc
    10621 gtgttcaacc gctctgcgaa ggccgcgttg taggcgatgg ccgccgcggt acgatcggcc
    10681 gccagctgcg tgcggccgtc ggcgctcagg ttgacggcct cggagaaatt gcccagcttc
    10741 ggcagcgtcg gcagcacgat gtagcgcgcg ccggcatcca ccagcgcctg ggcgatcgcg
    10801 gcgctcacat cggcggtctg ccgagggtca tcgacatcgc ggatatcgtt gcccgcgatc
    10861 agcgcgtagt agagcgtgtc gggtccaacg gtgagcgccc cggaggccac gcgcgccagg
    10921 aagcccggct gttcgacgcg aaagcctccc gcggacacca ccgagttgcc ggtcacggag
    10981 gcgagcactt cctccgagcg gtaaccgccg accgcgaaat tgaggttgtc cgtggccacc
    11041 gtgtcggtgc gcgcaccgaa caccagcagc ggtgtcgagg gattcatgcg cccgaggccg
    11101 aggtcgagcg ccagccattc cggcatcgag aggccgcgca ggcccgttgc cgggtcgata
    11161 ttggtgaagc gcagccccgt ggtgccgccc agctcgaggt cgggaaactg cccggagtcg
    11221 tcgtagctgg cgccgaaggc gacgaactgg ccgaatggca ccccggccca agcggcggcg
    11281 ctggccagcg acgccgcggc gatcgccgtc ctgatgagtc cccgcatccc atgctccccg
    11341 tgtgcgttgt ttgcgtgccc gctgcgcaac gaagatcacg aaccgggcgc gcgaacggat
    11401 gaaccgcatc atagccacaa gccggatgcc gccacaacgc acgctgcgcg gcgcgcgcct
    11461 gctgctgtac catgccgcgg tctccaggaa ggtcatcgat gcgaagcccc actgcctgca
    11521 ctctcgcttc attgctgttc gcgcagcgcg cactcgccga agcgccccgg ggcatcgacc
    11581 agcagatcgc ccagggcttc aaaccctttg ccgacgcggt atcgggcttc gtgttcgccg
    11641 ccatcccgtt cggcgatcac gcggtgccgt ggatcctgtt ctggctgatc gtcgccggca
    11701 tcttctgcac gctgtacttt cgcttcatca acctgcgcgg catggcgcag ggcatgcgca
    11761 tcatccgcgg cgactacgac gcgccgcacc accacggcga caccagccat ttccaggcgc
    11821 tggcgaccgc gctctccggc accgtgggac tcggcaacat cgccggcgtg gcagtggcgg
    11881 tgaccgtcgg cggcccggga gcggcgttct ggatgatcgt ggccgcgctg ctcggcatgg
    11941 ccaccaagtt ctgcgaatgc acgctggccg tgaagttccg ccaccgccgc cccgacggca
    12001 cggtctccgg cggcccgatg tactacctcg cgcagggcat cgcggccgaa tacccgcgcc
    12061 tcgcgccgct cggccgtctg ctcggcgtaa gcttcgcggt attctgcgtg tttggcacac
    12121 tgggctcggg caacttcttc caggtgaacc aggcctacca ccagctgctg ctgatcaccg
    12181 gcggcgatgc cagcgtgctg gcgggtcgcg cctggatgtt cggcatcgcg atggccgtgg
    12241 cgaccggcat cgtggtgctg ggcggcatcc agcgcatcgc gaaagtgacc gacaagctgg
    12301 tgccgctgat ggcgctgctc tatctcggcg ccggcctcat cgtgatcgcg catcacgtcg
    12361 acctgttgcc gcaggcgctg gcagcgatcg tcaacggcgc gttctcggcc gaaggcgtgg
    12421 caggcggcgt gctcggcgtg atactgcagg gtttccgccg cgcggccttc tccaacgagg
    12481 ccggcatcgg ctcggcgccg atcgcgcacg cgagcgtgcg caccgacgag cccctgaccg
    12541 aaggcctggt cgcactctgg gagccgttca tcgacaccgt gatcatctgc acgatgacgg
    12601 cgctggtgat cacgatctcc ggcgtcagcg ccgcgtcgac cggcgcgcag ggcgtcgctc
    12661 tcacctcggc cgccttcgcg acggtgatgc cctggttccc ctacgcgctc gccttggtgg
    12721 tgctgttgtt cgcctactcg acgatgatcg cctactgcta ttacgggacc aaggcggcga
    12781 actacctgtt cggcgagagc gccgccgtgg acgtctgcta caagctgttc tttctcggca
    12841 tgacggtatt cggtgtgacg atggacctgt cgcagctggt ggacttcgcc gacgcgatct
    12901 atttcctgat ggcggtgccg aacatcatcg ggctgtacct gctggcaccg gtggtgaaac
    12961 gcgaaatgga cagttacttc gcgcgcctcg cacgcggcga gatccgcccc ggccgcgagg
    13021 cgccggagca cctgctcgga tcgccctgac tcagtcggcc agcgccgcgt agacgttctg
    13081 cacgtcgtcg tcggcatcga gcgcctcgag aaaggcctcg acctcggcgc gcgctgccgc
    13141 gtcgagctgc accgggttgt tgggccggta gcccagcacc gcagactgca ccgtgaaacc
    13201 gaagcccggc agcgcgcggg tcaccgcatc gagatccgtg ggatcggtca ggaacagcgt
    13261 gacgttgtct tccaggggct cgaggtcctg cgcaccggcc tcgatggccg cgagctcggg
    13321 gtccgcatca ggcgcattcg gcgtggcctc gatcatgccg agatacttga agtcccacga
    13381 caccgcgccc gagttgccga gctggccctt gcgaaacagc atgcggatgc tcatcgcggc
    13441 gcggttcttg ttgtcggtga ggcactcgac gatcaccggc acctggtgcg gcgcgaaacc
    13501 ctcgaaggtc agggattcgt actgcacgtg ttcgtcgagc aggccggcac ccttcttgat
    13561 cgcgcgctcc agcgtgtcgc gcggcatcga ggcgcgcttg gcctgctcga cggccttgcg
    13621 cagccgcggg ttggaatccg ggtccgcgcc ggtgcgcgcg gcgaccatga tttccttggc
    13681 cagcttggtg aagatgcggc ccttcgtcgc cgccgcgtcg gcgcggccct tggctttcca
    13741 ctgagcgccc atgatctgtt cccctgaaaa aaccgcgcaa ttatagagcc cccggggcgg
    13801 cgcgccaccc ttcgatcgca aggtcaggcg cggcggttcc cggccagcgg cggcctgcag
    13861 agggtgtcgc aaaagccatt gcattgcgaa cgagcgggcg tggtgcgtcg ctggccgtgc
    13921 tgcccgccat tgtcctgcgg ggcacgcacg agtacgt
//
LOCUS       DAORUQ010000081        13838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4298_length_13838_cov_5.032794, whole genome shotgun sequence.
ACCESSION   DAORUQ010000081 DAORUQ010000000
VERSION     DAORUQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_13838_cov_5.032794"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1230)
                     /locus_tag="PLW13_08655"
     CDS             complement(64..1230)
                     /locus_tag="PLW13_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQY70484.1"
                     /translation="MNGIQYESIQEAALHFGVLMQTAARRLRSGWTPEQAFGVAKKRE
                     RVAHNATDIHTSRGTFNSIRALAEFVGIDEGTIANRLRYGWTHEQAAGFAEPPTPTRE
                     RRYNVVTCEGLQFNSWADLAANFGKREQLVSKRLRSGWTPEQAVDLEEPPPRFRRFDG
                     SPRNHLWREPTVLDGKLLPGAPAGSYRLYVVRNKVNNREYIGITTNDLKARWRGHVRL
                     AKLGRKSKFYNAIRKYGSKNFLIELVRSDARDFSELQAQEMAEITVRNTISKGYNTAA
                     GGSVGTSKPIEIAGTLFPSHQAAAGHYGVDAKIFNQRMSEIGWTPEEAVGLVNRRFSR
                     RSIEINGKRFASLKSAAEALGLKPGTVQARITRSGMSFEEALGLKVSSRLRRGR"
     gene            complement(1437..1622)
                     /locus_tag="PLW13_08660"
     CDS             complement(1437..1622)
                     /locus_tag="PLW13_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70485.1"
                     /translation="MSRINDDDLPLTQAQLIAAIRGALGILDKLIELEAFPDRVDEAV
                     AVLTEMLSAYIRRRRVH"
     gene            1890..2246
                     /locus_tag="PLW13_08665"
     CDS             1890..2246
                     /locus_tag="PLW13_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQY70486.1"
                     /translation="MRFSVEAPVRVTVSECNCSICAMTGFLHLIVPAARFRLLTGADV
                     LTTYTFGTGVAKHLFCRHCGIKSFYVPRSNPDGYSVNLRCLDKGTIEHVDIEPFDGQN
                     WEQGAARLAHLSQDSG"
     gene            2272..2937
                     /locus_tag="PLW13_08670"
     CDS             2272..2937
                     /locus_tag="PLW13_08670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQY70487.1"
                     /translation="MNDHGSLAIRACTADDAMAAVPLIHDSGPIAFRYVFSHRHEAQS
                     LEFLRAAFVDGDGEFGFRNHLVAERAGAVIAVAALWDARANLRFTVAAARQILGFYRT
                     AAPAVILRGLRMERVVKPAAAGVAYIGHVAVAPALRGQGAGRALLGHLLGRARHEGYA
                     RAALDVAATNPRARALYEGLGFRTVATRRSALQGPWGRVPDHHYMVLDLGSERWHGGT
                     PGI"
     gene            2967..3581
                     /locus_tag="PLW13_08675"
     CDS             2967..3581
                     /locus_tag="PLW13_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY70488.1"
                     /translation="MRKLIHATVWLLLLSGLTGCGYNTLQSADEQIKSSWAEVLNQYQ
                     RRADLVPNLVNVVKGYAAHEKEVLTRVTEARAKVGSVQATPELINDPEAFARFQAAQG
                     ELQSALSRLLVVAENYPNLKADAGFRDLQAQLEGTENRITVARNRYIQAVEQYNVTVR
                     RFPGNLTAMLFGFEVKPQFTVENEREMATPPVVDFGTTPAPQAN"
     gene            3586..4467
                     /locus_tag="PLW13_08680"
     CDS             3586..4467
                     /locus_tag="PLW13_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY70489.1"
                     /translation="MRAALARLLLALLLAATFAVAEVPVPALEARVTDLTGTLTTQQR
                     GALEESLRAFEALKGAQLAVLLVPTTAPETIEQYAIRVAEAWKLGRQGVDDGLLLLVA
                     TQDRRMRIEVGYGLEGAVPDAIAKRVIAETITPYFRQGDVPGGIEAGMQQLMRVVDGE
                     PLPPPAPAAGGSGVSPDLLVFGFFFLVVAGTVLRGIFGRLLGSGVAGGIMGMIVWAAS
                     HALLMALGAALLMFVLVLLFGGGRGGRGGWSSGGFGGGRGGFGGGGFGGGGGFGGGGG
                     FGGGGGGFGGGGASGGW"
     gene            4467..4958
                     /locus_tag="PLW13_08685"
     CDS             4467..4958
                     /locus_tag="PLW13_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY70490.1"
                     /translation="MEWLRVLRHLAMPAWRLRQSLPRASMQAIETAIGASERTHGGQI
                     CFAVETALDLAPLARGQAARERALEVFAQLRVWDTEHNNGVLIYLLLADRDVEIVADR
                     GIAARVPAAQWEAICQAMESAFRAGRFEQGALDGIRAVGELLARHYPERGADTLSNAP
                     YLL"
     gene            5060..5989
                     /locus_tag="PLW13_08690"
     CDS             5060..5989
                     /locus_tag="PLW13_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70491.1"
                     /translation="MALTGCSILVTGPAGQIAFPLAARLARDNEVWGIARFADAASRR
                     RVEEAGITTRAVDLAAPDWSGLPERFDYVLHLAAVIPPGLDYDATMRVNAGGTGHLMR
                     RFRNARACLVMSTCGVYQPPDDPAHAVRESDPLGVSHQPYAPTYCVSKIAQEAVARFA
                     AEAYGLPTTIARMNAAYGDNGGLPAILLEMILAGRPIPLPTGRAAIASPIHEDDIFAH
                     TPGLLAAAAVPATITNWGGDEPVDVRDMAAHLAALAGREVRFLESPDGMTQFRLDAAR
                     RRELAGPCHVDWREGMRRMVAARHPELLRAPPA"
     gene            6095..7555
                     /locus_tag="PLW13_08695"
     CDS             6095..7555
                     /locus_tag="PLW13_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY70492.1"
                     /translation="MDEFDVCVIGAGYGGATVAALLAQAGRRVALLEKTARAGGKTQT
                     TERKGYHFEMFGAVGIPALNSRFHELVDRLGIAGRVPFVVPEGNAAAVRYKTPDGTWR
                     TMRTPLIASGSPEEMANLKRVYGVDDEALAALGNFYGALFAVDDAQLEALENVGMLAW
                     MTQFGLATPLVSQICMSLNTLFVVAVDRLAASEAIPVLRAQVLGGAGRYHLGGYGKVA
                     EACADYVVEHGGAWFSKCRARRILVENGRAVGVLTDAGTIRARAVISNAGIQPTVLDL
                     AGREHFPADYVRRVESLEPSWAIAGYRYVLDKRVFDAALIPVFSDQSWLDTARFEAMA
                     RGEWPEVPLIAIDVPSEFDPSLVPVPGHQVANCQVFCSADPGSAQWEEAIRRADAVLA
                     ELWPDLEQHILRKEPYGPKQVSGMSRDSAVPGAGGEAVGIAQVIGQVGASKPAARTPL
                     AGLYLVGCDAGGTGAGTHQAVDSGFRVAQLVDSDLA"
     gene            7764..9293
                     /locus_tag="PLW13_08700"
     CDS             7764..9293
                     /locus_tag="PLW13_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HQY70493.1"
                     /translation="MSSLSERFALRSPVFESLEQDLASADSYEQWHQLAARHDRLSGA
                     EEWRRNDASRLYDHENIRLRLQQLRRLRKKGDDHGLLFVLNEGIHGNMEGMGKAQLYM
                     RAKCGTKKLIEDYITEISAALEYLAPRDFEGIPWAERMEFYQRASHCYGRSALMLSGG
                     GTLGYFHLGVLKALIEQGLCPAVISGASAGAFVAAIVGTRTDEEFLALFEDNYLARAL
                     TANPDNIKIGFGSKKSIDMQAVKREMQRLIPDMTFLEAYRKTGRSINITISPSSARQT
                     SRLLNHIASPNVTVLSAVLASSALPGVFAPVQLEARNAVGEIRPYLPSRRWIDGSFSQ
                     DLPAKRLGRMYGVNHFIVSQVMPGLGREPNQSVGMRQIVRDTFVAATKEVVRGSLDWI
                     QRRTAVGPRLGMALNTVNTLIDQQFVGDINIFPGYGMSNLGMILRMLSEDEMAHLFKS
                     GERATWPKIPAIAMTGRIGRTLDGILHEFEARESHWLRASRRPHAAPNPPPRRVGAKA
                     A"
     gene            9337..10044
                     /locus_tag="PLW13_08705"
     CDS             9337..10044
                     /locus_tag="PLW13_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HQY70494.1"
                     /translation="MRQLRHRLDSRPALARVLPFAVFLGLTVLQGRLGPDSLYWVYLF
                     KTLLGAFLVWLVWPLVLEMRWRFGWDALLTGVLVFALWVTLGQGWGTQQGLWAALSGQ
                     VATGSPAPAWNPLAAFGDAPALAWFFVVVRVIGSSIVVPPIEEAFYRSFVYRSLLAGG
                     FDKVPLGHFAWRPFLFTALVFGFAHQQWLAGILCGAAYQWLVIRHARLGEAMCAHAIT
                     NFLLGIWVVTTGAWQFW"
     gene            10125..11045
                     /locus_tag="PLW13_08710"
     CDS             10125..11045
                     /locus_tag="PLW13_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70495.1"
                     /translation="MNAELQPLNARFSALHIDVARFATDDFNPFHDRNKWQRIEGNPF
                     GGAIALGFQLSNFALHGVSMLHAAAPLQQRYLHVRITFADAVRVDDDVMLAIKPAQLG
                     GDGATVSHRIALRTERSLALTGHIRFVADAPADPPTPVEPSRLNDIPDRSDVPGTEYF
                     LKRKFMSTANAKNFLLGANVDPAVYFDELEDRVRFTELFPVSLVSCALLERGTRHGHD
                     FLRAPMVYSYHDIVVDRDVALRLRSNDRLNMLVSAGQPPGDVPTTGLRREQRVHLCLG
                     YTGAGERLFSAEIGLVPLSSLAAQDPVSPR"
     gene            complement(11042..11815)
                     /locus_tag="PLW13_08715"
     CDS             complement(11042..11815)
                     /locus_tag="PLW13_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70496.1"
                     /translation="MDQPRYDLRGRIAFISGASAGIGAHLARLYAAAGAAVVLGARRI
                     ERCEALAAQIGSEGGRALAVALDVAQEPSVIAAYDAAEAAFGAPDVVVANAGIGTGGR
                     STDVPLERLRALVDTNFTGLYLTVREGAKRMIAAGSRESQRGRIILIGSMTAHMSNQG
                     DAAYAALKAGVEHLGRQFAREWIRLGINVNTVHPGIIPTASNADWFAGARGQAQVAGL
                     PRRRLVDLGALDDIMLLLASDASRQLTGTAITIDDGQSL"
     gene            complement(11835..>13838)
                     /locus_tag="PLW13_08720"
     CDS             complement(11835..>13838)
                     /locus_tag="PLW13_08720"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQY70497.1"
                     /translation="VLFKKFANIDVFDIEIDEKNVDKLVDIIASLEPTFGGINLEDIK
                     APECFLVEQKLRERMKIPVFHDDQHGTAIVAGAAVYNALRIVGKKIEDVKMVVSGSGA
                     ASIACVDLLVTMGLDPAHVTLVDRSGVVYTGRTEGMNPWKQKYARDTSLRTLDDAMDG
                     ADIFMGLSGPGVLKAAAVKKMAERPLILAMANPTPEIMPEEALAVRPDAILATGRSDY
                     PNQVNNVLCFPFIFRGALDCGASTINDAMKAACVKAIAGLAEKEVPNEVALAYAGETL
                     KFGPDYLIPKPFDPRLIEEVPVAVVKAAMESGVATRPIADLEAYRRKLHEFVNRSGLF
                     MQPFIDVARKNQTRIVYAEGENEDVLLAVQSVVDEGVAQPILIGRPDAIQAQMDRLSM
                     HLEIGRDVQVFNPEESDSKPEYWQHYHALVGRQGVSVEAAQIAMRTQASALAAVMVAR
                     GEADGLICGKVGAFTDHLRTIRGVLGNGGSDHRHVSSLCALLLPSGPLFLADAFLTFD
                     PTVEQVVETTKNSIELVRHFGVTPKVALLAHSNFGTSNAPSALKMREAAGMLREQLPE
                     VELDGEMHSFTAMTETLRKTIYQDSKLSGAANLLIMPNIDAANIALGLIRSLTDALLI
                     GPLFTGFTRPAHVVIPSVTSRGIFNMSAFTSAEIHRAREREQG"
BASE COUNT         2136 a         4583 c         4728 g         2391 t
ORIGIN      
        1 atcggtgcgg cggacactct gtcgccagga cggcgacagg tagcctgcag gggtgcgcgt
       61 gaattatcgc ccccggcgca atctacttga aaccttcaat ccaagcgctt cctcaaaaga
      121 cattcctgaa cgcgtgattc gggcttgaac ggttcccggc ttcaatccca aagcttcagc
      181 tgcggatttc agggaggcga aacgctttcc gttgatctca atcgatcgtc tgctaaaccg
      241 tcgattgacc agaccaaccg cttcttcagg cgtccatccg atttcactca tacgttggtt
      301 gaaaatcttg gcgtcaacgc catagtgtcc tgccgctgcc tgatgcgatg gaaatagcgt
      361 tccggcaatc tctatcggct tggatgtccc taccgatccg ccagccgcgg tgttgtaacc
      421 tttgctaatc gtattgcgga cagtaatctc cgccatttct tgagcctgca attccgagaa
      481 atcgcgcgcg tcgctccgaa caagttcgat caaaaaattt ttcgatccgt attttcgaat
      541 cgcgttatag aactttgact tccgccccag tttcgcgagc ctgacgtgtc cgcgccagcg
      601 cgccttcaaa tcgtttgtag tgattccgat gtattcgcgg ttatttactt tgttcctgac
      661 aacgtaaagc cgatagctcc cggcgggtgc gccgggtaaa agttttccgt cgagcaccgt
      721 cggttcgcgc cataagtggt tcctcggaga accgtcaaaa cggcggaatc gtggaggtgg
      781 ctcctcaaga tcaactgctt gctccggtgt ccaacccgat cgcaatcgtt tcgatactag
      841 ctgttcccgt ttaccgaaat tagcggccag gtcagcccaa ctgttgaatt gcaacccctc
      901 gcacgtcacg acgttatatc gtcgctcgcg ggtcggcgtg ggtggttccg caaaacccgc
      961 cgcttgttca tgcgtccaac cgtatcgaag ccgattcgca atcgtgcctt catctatccc
     1021 gacgaattct gcaagcgctc ggatcgaatt gaaggtgccg cgagacgtat gtatatctgt
     1081 cgcgttatgc gcgacgcgct cccgcttctt tgcaacgccg aacgcctgtt ccggcgtcca
     1141 acccgaacgg agtcgacgag cagcggtttg catcagcact ccaaagtgca gagcagcctc
     1201 ctgaatagat tcgtattgaa taccgttcac gataacggtt gttttgattc ccataagtcg
     1261 cggcgactgg atgatgaagg ctgactaata ttagcagcag atggccgccc aaacgatcaa
     1321 cggatcaagc gcggcgatcg tcgtcggagg atttccttta cggtcgatga actgcgcgct
     1381 ctccggcgcg aggctataac gttgatagac tgaccccgac cagcgcacca cgccgatcag
     1441 tgtacccggc gacgcctgat gtatgcggaa agcatttcgg tcaggacggc gacggcttcg
     1501 tctacgcgat cgggaaacgc ctcaagctcg atcaatttgt cgaggatgcc gagcgcgccg
     1561 cgaatcgcgg cgatcaattg cgcttgcgta aggggcagat cgtcgtcgtt gattcgcgac
     1621 aagcttaggc catcaccacg ctctcgaccg gcctttcgtc gcgggcttcg ccccggacat
     1681 tgaccgactc gaccgcgcgc ccgacgtcgg cttcgacctc acccccgagc cgtagccgga
     1741 actggctgtg gccgtagcag gaaccggcta tgctgcatgc ctagatgccc ccgacgacta
     1801 agtgtgggga tggacgaaaa tgcgggcgat gaaatcatga ggagtagacg ccaatgccaa
     1861 cccattttgg tggctgtcac tgcgggaaag tgaggttttc tgttgaggcg ccggtgcgcg
     1921 tcacggtgag cgagtgcaac tgctcgatct gcgccatgac gggcttcctg cacctgatcg
     1981 tgccggccgc gcgctttcgg ctgctcaccg gcgccgacgt gctgacgacc tacaccttcg
     2041 gcaccggggt ggcgaagcac ctgttctgtc gccactgcgg tatcaaatcg ttctacgtgc
     2101 cgcgctccaa ccccgatggc tacagcgtga acctgcgctg cctcgacaag ggcacgatcg
     2161 agcacgtcga catcgagccc ttcgacgggc agaactggga gcagggcgcc gcgaggctgg
     2221 cgcatctgtc gcaggacagc ggctgagccg gtggacgccg cgggcgccgt catgaacgat
     2281 cacggctcgc tcgcgatccg tgcctgcacc gccgacgatg cgatggccgc ggtaccgttg
     2341 atccacgatt cgggcccgat cgcctttcgc tatgtctttt cgcatcgtca cgaggcgcag
     2401 tcgctggaat tcctgcgcgc cgcctttgtc gacggcgatg gcgagttcgg gtttcgcaac
     2461 cacctggtgg cggagcgcgc gggcgcggtg atcgccgtcg cggcgctgtg ggacgcgcgc
     2521 gccaacctgc gcttcacggt ggcggcggcg cgccagatcc tcggtttcta ccgcaccgcg
     2581 gcgcccgcgg taatcctgcg cgggctgcgc atggaacgcg tcgtgaaacc ggcggcggcc
     2641 ggcgtcgcct acatcgggca cgttgcggtc gcgccggcgc tgcgcgggca gggcgcgggc
     2701 agggcgctgc tcggtcatct gctcgggcgc gcgcgtcacg agggctacgc gcgtgccgcg
     2761 ctggacgtgg ctgccaccaa tccgcgtgcc cgcgcgcttt acgaggggct gggctttcgc
     2821 accgtcgcga cgcgccgttc ggcactgcag ggcccgtggg gcagggtgcc tgatcatcat
     2881 tacatggtgc tcgacctcgg gagcgagcgc tggcacggcg ggacgccggg gatataatcc
     2941 ccgcgcacac tcacaaggag attcccatgc gcaagctgat acacgccacg gtgtggctgc
     3001 tgttgctgag cgggttgacg ggctgcggct acaacacgct gcagagtgca gacgagcaga
     3061 tcaagtcgag ctgggccgag gtgctgaacc agtaccagcg ccgcgccgac ctggtgccga
     3121 acctggtcaa cgtggtcaag ggctacgccg cgcacgagaa ggaggtgctc acgcgcgtga
     3181 ccgaggcgcg cgcgaaggtg ggcagcgtgc aggccacgcc cgagctcatc aacgaccccg
     3241 aggccttcgc gcgcttccag gcggcgcagg gcgagttgca atcggcgctg tcgcgcctgc
     3301 tggtggtcgc cgagaactac ccgaacctca aggccgacgc cggctttcgc gacctgcagg
     3361 cgcagctcga aggcaccgag aaccgcatca ccgtggcgcg caaccgctac atccaggcgg
     3421 tcgagcagta caacgtgacg gtgcgacgct tccccggcaa cctcaccgcg atgctgttcg
     3481 gcttcgaggt gaagccgcag ttcacggtcg agaacgagcg ggagatggcg acgccgccgg
     3541 tggtcgattt cggcacgaca ccggcaccgc aggcgaactg atccggtgcg agccgcactc
     3601 gcgcgcctgc tgctggcgtt gttgctcgcg gcgacattcg cggtcgccga agtgccggtg
     3661 cccgcgctcg aggcacgcgt cacggatctg accggcacgc tgacgacgca gcagcgcggt
     3721 gcgctggaag agtcgctgcg cgccttcgag gcgctgaagg gcgcgcagct cgcggtgctg
     3781 ctggtgccca cgacggcgcc agagaccatc gagcagtacg cgatccgcgt ggctgaggcc
     3841 tggaagctcg gacgccaggg cgtcgatgac ggcctgttgc tgctggtggc gacgcaggac
     3901 cgtcgcatgc gcatcgaggt aggctacggc ctcgagggcg cggtgcccga tgcgatcgcc
     3961 aagcgcgtga tcgccgagac catcacgccg tatttccgcc agggcgacgt ccccggcggc
     4021 atcgaggccg gcatgcagca gctgatgcgc gtggtcgacg gcgagccgct gccgccgccg
     4081 gcacccgcgg cgggcggcag cggcgtttcc cccgatctgc tggtcttcgg tttcttcttc
     4141 ctggtggtcg cgggcacggt gctgcgcggc atcttcgggc gcctgctggg ctcgggcgtc
     4201 gccggcggca tcatgggcat gatcgtgtgg gcggcaagcc acgcgctgct gatggcgctc
     4261 ggcgcggcgc tgctgatgtt cgtgctcgtg ctgctgttcg gcggcggccg gggtggtcgc
     4321 gggggctggt ccagcggtgg ttttggcggc gggcgcggcg gcttcggtgg tggtggtttt
     4381 ggcggtggag gcggcttcgg cggcggcgga ggttttggcg gcggcggtgg cggcttcggt
     4441 ggcggtggcg cctcgggagg atggtgatgg agtggctgcg cgtgctgcgg cacctggcga
     4501 tgcctgcgtg gcgcctgcga cagtcgctgc cgcgcgcgtc gatgcaggcc atcgagacgg
     4561 cgatcggcgc ctccgagcgc acgcacggcg ggcagatctg cttcgcggtc gagaccgcgc
     4621 tggacctcgc gccactcgcg cgcggacagg cggcgcgcga gcgcgcgctg gaggtgttcg
     4681 cgcaactgcg ggtgtgggac acggagcaca acaacggcgt gttgatctac ctgctgctgg
     4741 ccgatcgcga cgtggagatc gtggccgatc gcggcattgc cgcgcgggtt ccggcggcgc
     4801 agtgggaggc gatctgccag gccatggaaa gcgcattccg cgcggggcga ttcgagcagg
     4861 gcgcgctgga cggcatccgc gcggtgggcg agctgctggc gcggcactac ccggagcgcg
     4921 gcgccgacac gctctcgaac gctccgtacc tgttgtagga gctggccatg ccagcgacgg
     4981 cagcgctcgg gtgccctgcc gcagataaac cagaatgccg cgggcatgcg ccgcgcctct
     5041 tgaagaaagg aagatgacaa tggcattgac gggctgcagc atcctggtca ccggaccggc
     5101 cggccagatc gcgtttccgc tggcggcgcg cctcgcgcgc gacaacgagg tgtggggcat
     5161 cgcgcgtttc gccgatgccg cgtcgcgccg gcgcgtggag gaggccggca tcacgacccg
     5221 cgccgtcgac ctcgccgcgc ccgactggag cggactgccc gagcgcttcg actacgtcct
     5281 gcacctggcg gccgtgatcc cgcccggcct cgactatgac gccacgatgc gcgtcaacgc
     5341 cggcggcacg ggacacctga tgcggcgttt ccgcaacgcg cgcgcctgcc tggtgatgtc
     5401 gacctgcggc gtgtaccagc ccccggacga tccggcgcac gcggtgcgcg agagcgatcc
     5461 gctcggcgtg agccaccagc cctatgcgcc gacctactgc gtgtccaaga tcgcgcagga
     5521 ggccgtggcc cgtttcgcgg ccgaggccta cgggctcccg accacgatcg cgcgcatgaa
     5581 cgcggcctac ggcgacaacg gcggactgcc ggcgatcctg ctcgagatga tcctggccgg
     5641 gcggccgatt cccttgccga cgggccgcgc cgcgatagcg agcccgatcc acgaggacga
     5701 catcttcgcg cacacgcccg ggctgctggc cgcggccgcc gtgcccgcga cgatcacgaa
     5761 ctggggcggc gacgagccgg tggacgtgcg cgacatggcc gcgcaccttg ccgcgcttgc
     5821 cgggcgcgag gtgcgattcc tcgagtcgcc tgacggcatg acgcagttcc gcctcgatgc
     5881 cgcgcgccgg cgcgagttgg ccggcccctg ccacgtcgac tggcgcgagg gcatgcggcg
     5941 catggtggcg gcgcggcacc cggagctgct gcgcgcgcca cccgcttgag cgccgccgcg
     6001 cgatcctgag caaaccgtaa tcgcgcccgg tcgccctgcg gccggataat ccgtgcacca
     6061 tcaccatcac catcaccggc gcggggcgcg gaacatggac gaattcgatg tgtgcgtgat
     6121 cggcgcgggc tacggcggcg ccacggtggc ggcgctgctg gcccaggccg ggcggcgcgt
     6181 ggccttgctg gagaagaccg cgcgagccgg gggcaagacc cagaccaccg agcgcaaggg
     6241 ctatcacttc gagatgttcg gcgcggtcgg cattccggcg ctgaactcgc gcttccacga
     6301 actcgtcgac cggctcggca tcgccggacg cgtacccttc gtggtgcccg agggcaacgc
     6361 ggccgcggtg cgttacaaga cacccgacgg cacgtggcgc acgatgcgca cgccgctgat
     6421 cgccagcgga tcgcccgagg agatggcgaa tctgaagcgc gtctacggcg tcgatgacga
     6481 ggcgctcgcg gcgctcggca atttctacgg cgcgctgttc gcggtcgacg acgcgcagct
     6541 ggaggcgctg gagaacgtcg gcatgctggc gtggatgacg cagttcgggc tcgccacgcc
     6601 gctcgtgagc cagatctgca tgagcctgaa cacgctgttc gtggttgcgg tggaccggct
     6661 cgcggcctcc gaggcgattc ccgtgctgcg cgcgcaggtg ctgggcggcg cgggccggta
     6721 tcacctcggc ggctatggca aggtggccga agcctgcgcc gattacgtcg tggagcacgg
     6781 cggggcctgg ttcagcaagt gccgcgcgcg gcggattctc gtcgagaacg gccgcgcggt
     6841 cggcgtgctc accgacgcgg gcacgatccg ggcgcgcgcc gtgatctcga acgccgggat
     6901 ccagccgacg gtgctcgacc tggccgggcg cgagcatttc ccggcggact acgtgcgccg
     6961 cgtggaatcg ctggaaccct cgtgggccat cgcgggttac cgctacgtgc tcgacaagcg
     7021 cgtcttcgat gccgcgctga tcccggtgtt ctccgaccag agctggctcg acacggcgcg
     7081 cttcgaggcc atggcgcgcg gcgagtggcc cgaggtgccg ctgatcgcga tcgatgtgcc
     7141 ctcggaattc gatccctcgc tggtgccggt gccggggcac caggtggcga actgccaggt
     7201 gttctgctcg gcggatcccg ggtccgcgca gtgggaggag gcgatacggc gcgccgatgc
     7261 cgtgctggcg gaactctggc cggatctcga acagcacatc ctgcgcaagg agccctacgg
     7321 gccgaagcag gtctcgggca tgagccgtga ttccgcggtg cccggtgccg gcggcgaggc
     7381 cgtgggaatc gcgcaggtga tcgggcaggt gggggcctcg aagcccgcgg cgcgcacacc
     7441 gctcgcgggt ctttacctgg tgggctgcga cgccggcggc accggcgcgg gcacgcacca
     7501 ggcggtggat tccggcttca gggtcgcgca gctggtggat tccgacctgg cataggcgca
     7561 ggcaggggtg ggagctgcgc gatttccacc gggcgaacgc gggcccggat ttgatccggc
     7621 tcaagcactg gcgatcgggt tcttgcgggg agcgcagcga acttgcggca cggcgagctt
     7681 tgctgtacca aagatgtttt ggcgcggcac gggacgctcg atgtccggcg tcactggctg
     7741 cccgccgcca tccggaggtt tcgatgtcct ctctcagcga acgattcgcg ctccgttccc
     7801 ccgtgttcga atccctcgag caggacctgg cgagcgccga tagctacgag cagtggcacc
     7861 agctggccgc gcggcacgac cggctgagcg gtgccgaaga gtggcggcgc aacgacgcct
     7921 cgcggctgta cgaccacgag aacatccgcc tgcggctgca gcagctgcga cgcctgcgca
     7981 agaagggcga cgaccacggt ctcctcttcg tgctgaacga gggcatccac gggaacatgg
     8041 agggcatggg caaggcgcag ctgtacatgc gcgccaagtg cggcaccaag aagctgatcg
     8101 aggattacat caccgagatc agcgccgcgc tggagtacct ggcgccgcgc gacttcgagg
     8161 gcatcccctg ggccgagcgc atggagttct accagcgcgc cagccactgc tacgggcgct
     8221 cggcgctgat gctgagcggc ggcggcacgc tgggctattt ccacctcggc gtgctgaagg
     8281 cgttgatcga gcaggggctc tgcccggcgg tgatttccgg cgccagtgcc ggtgcgttcg
     8341 tggccgccat cgtcggcacc cgaaccgacg aggagttcct cgcgctgttc gaggacaact
     8401 accttgcacg ggcactcacg gctaaccccg acaacatcaa gatcggcttc ggcagcaaga
     8461 agagcatcga catgcaagcc gtgaagcgcg agatgcagcg gctcatcccg gacatgacct
     8521 tcctcgaggc gtaccgcaaa acggggcgca gcatcaacat caccatctcg ccttccagcg
     8581 ccaggcagac ctcgcggctg ctcaaccaca tcgcctcgcc caacgtgacc gtgctctcgg
     8641 cggtgctcgc cagctccgcg ttgcccggcg tgttcgcgcc ggtgcagctc gaggcgcgca
     8701 acgcggtggg cgagatcagg ccctacctgc cgtcgcggcg ctggatcgac ggctcgttct
     8761 cgcaggacct gccggccaag cgcctcgggc gcatgtacgg cgtcaatcac ttcatcgtca
     8821 gccaggtgat gccgggcctg gggcgcgagc cgaaccagtc ggtgggcatg cgccagatcg
     8881 tccgcgacac gttcgttgcc gccaccaagg aagtggtgcg cggcagcctc gactggatcc
     8941 agcggcgcac ggcggtcggc ccgcggctcg gcatggcgct gaataccgtg aacaccctga
     9001 tcgaccagca gttcgtgggc gacatcaaca tattccccgg ctacgggatg tcgaacctgg
     9061 gcatgatcct caggatgctg agcgaggacg aaatggccca cctgttcaaa tccggcgagc
     9121 gcgccacctg gcccaagatt cccgccatcg ccatgaccgg gcgcatcggc cgcacgctgg
     9181 atggcatcct gcacgagttc gaggcgcgcg agtcgcactg gctgcgcgca tcgaggcgcc
     9241 cgcatgcggc gccgaacccg ccgccccggc gcgtaggcgc caaagccgcc tgatgccggg
     9301 cgaggtgcgc gagcggcctg ttgcacgacc gctcgcatga ggcaactgcg ccatcgcctg
     9361 gattcccgtc ccgcactggc gcgcgtgctg ccctttgccg tgttcctggg gctcacggtg
     9421 ttgcaggggc gtctcggccc tgactcgctg tactgggtct acctgttcaa gacgctcctc
     9481 ggtgcgttcc tggtgtggct ggtgtggccg ctggtcctgg agatgcgctg gcgcttcggc
     9541 tgggacgcgc tgctgaccgg cgtgctggtg ttcgcgctgt gggtgacgct cggccagggg
     9601 tgggggacgc agcaagggct gtgggccgcg ctgtccggcc aggttgcgac cggctcaccg
     9661 gcgccggcgt ggaacccgtt ggccgccttc ggcgacgcgc cggcccttgc gtggttcttc
     9721 gtggtggtgc gcgtgatcgg ctcgagcatc gtcgtgccgc ccatcgagga ggcgttctac
     9781 cgctcgttcg tgtaccgctc gctgcttgcc ggcggattcg acaaggtgcc gctcggccac
     9841 tttgcatggc gccccttcct gttcaccgcc ctcgtgttcg gtttcgcaca ccagcaatgg
     9901 cttgccggca tcctgtgcgg cgccgcatac cagtggctgg tgatccgtca cgcgcgcctc
     9961 ggcgaggcga tgtgcgcgca cgcgatcacg aatttcctgc tggggatctg ggtggtgacg
    10021 acgggcgcct ggcagttctg gtagtcgttg ccccgctgcc cgggtcaacg ccagcgcgcg
    10081 gctatactcg attgcaccat gacaatcgat cgggtacccg gggcatgaac gcagaactgc
    10141 aaccgctgaa cgcgcgcttt tcggcactgc acatcgatgt cgcgcgtttc gcgaccgacg
    10201 atttcaaccc gttccatgat cgcaacaagt ggcagcgcat cgagggcaac cccttcggcg
    10261 gggcaatcgc gctcggcttc cagctctcga actttgcgct gcacggtgtc tcgatgctgc
    10321 acgcagccgc gccgctgcag cagcgctacc tgcacgtgcg catcaccttc gccgatgcgg
    10381 tgcgcgttga cgacgacgtg atgctcgcga tcaagccggc gcaactcggc ggggatggcg
    10441 ccactgtcag tcaccgcatc gcgctgcgta cggagcgcag cctcgccctg acgggccata
    10501 tccgcttcgt ggcggacgca ccggccgatc cgcccacacc ggtggaaccg tcgcgattga
    10561 atgacatccc ggatcgcagc gacgtgccgg gaacggaata cttcctcaag cgcaagttca
    10621 tgagcacggc gaacgccaag aatttcctgc tgggcgcgaa cgtggatccc gccgtctatt
    10681 tcgatgaact cgaagaccgc gtccgcttca ccgaattgtt ccccgtcagc ctggtttcct
    10741 gcgcgctgct cgagcgcggc acccggcatg gccacgattt tctgcgggca ccgatggtgt
    10801 attcctatca cgacatcgtg gtcgaccggg atgtcgcctt gcgcctgcgc agcaacgaca
    10861 ggctcaacat gctggtttcg gctggccagc cgccgggcga cgtgcccacc accggtctgc
    10921 gccgggagca gcgcgtgcac ctgtgtctcg gctacaccgg tgccggtgag cgcctgttca
    10981 gcgccgagat cggcctcgtg ccgctgagtt cgctcgccgc gcaggacccg gtatcccccc
    11041 gctagagcga ttgcccgtca tcgatggtga tcgccgtgcc cgtgagctgt cgcgaggcgt
    11101 ctgaggccag cagcagcatg atgtcatcga gcgcgcccag gtcgaccagg cgccggcgcg
    11161 gcaggccggc gacctgggcc tggccccgcg cgccggcgaa ccagtccgcg ttgctcgcgg
    11221 tcggtatgat gccggggtgc acggtgttga cgttgatccc gaggcggatc cattcgcgcg
    11281 cgaactgccg cccgaggtgc tccacccccg ccttcagcgc ggcataggcc gcatcgccct
    11341 ggttgctcat gtgcgcggtc atcgacccga tcaggatgat gcgcccgcgt tggctctccc
    11401 ggctgcccgc ggcgatcatc cgcttcgcgc cttcgcgcac ggtgaggtag agtccggtga
    11461 aattcgtgtc gaccagcgcg cgcagccgct ccagcggcac atcggtcgat cgcccgccgg
    11521 tgccgatgcc ggcgttggcg actaccacgt ccggcgcgcc gaaggcggcc tcggccgcgt
    11581 cgtaggccgc gatgaccgac ggctcttgcg ccacgtcgag cgccacggcc agcgcgcgcc
    11641 cgccctcgga cccgatctgc gccgccagcg cttcgcagcg ctcgatgcgc cgtgccccca
    11701 gcaccaccgc agcgcccgcc gccgcgtaga gccgggcgag atgcgcgccg atgccggccg
    11761 aggcgccgga gatgaacgca atgcgtccgc gaagatcgta gcggggctgg tccacgcgcg
    11821 atcccgttgc gctctcagcc ctgctcgcgt tcccgcgccc ggtgaatctc cgccgaggtg
    11881 aaggcgctca tgttgaagat gccgcgcgag gtcacggagg ggatcaccac gtgcgcgggc
    11941 ctggtgaaac cggtgaacag cggtccgatc aacagcgcat cggtcagcga acggatcagt
    12001 cccagcgcga tattggccgc gtcgatgttc ggcatgatca gcaggttcgc ggcaccgctc
    12061 agcttcgagt cctggtagat cgtcttgcgc agcgtctcgg tcatcgcggt gaacgagtgc
    12121 atctcgccgt cgagctccac ctcgggcagc tgctcgcgca gcatcccggc ggcctcgcgc
    12181 atcttcaggg ccgaaggggc attcgaggta ccgaagttcg aatgcgcgag gagtgcgacc
    12241 tttggtgtga cgccaaagtg gcgcaccagc tcgatgctgt tcttcgtggt ctccaccacc
    12301 tgttcgaccg tcgggtcgaa ggtgaggaaa gcatcggcca ggaacagcgg gccgctcggc
    12361 agcagcagcg cgcacagcga cgacacgtgg cggtgatcgc tgccgccgtt gccgagcacg
    12421 ccgcggatgg tgcgcaggtg gtcggtgaat gcgcccacct tgccgcagat caggccgtcg
    12481 gcttcgccgc gcgccaccat caccgccgcc agggccgagg cctgcgtgcg catcgcgatc
    12541 tgcgcggctt ccaccgacac gccctggcgc ccgaccagcg cgtgatagtg ctgccagtac
    12601 tcgggcttgc tgtcggactc ctcggggttg aatacctgca cgtcgcgccc gatctcgagg
    12661 tgcatcgaga gccggtccat ctgcgcctgg atcgcatcgg gacggccgat caggatcggc
    12721 tgcgccacgc cttcgtcgac caccgactgc accgcgagca gcacatcctc gttctcgccc
    12781 tcggcgtaga cgatgcgggt ctggttcttg cgcgccacgt cgatgaaggg ctgcatgaac
    12841 aggccggagc ggttcacgaa ctcgtgcagc ttgcgccggt aggcctcgag atcggcgatc
    12901 ggtcgcgtcg cgacaccgct ctccatcgcg gccttcacca ccgcgaccgg cacttcctcg
    12961 atcaggcgcg ggtcgaaggg cttggggatc aggtagtcgg ggccgaattt cagcgtctcg
    13021 ccggcatagg ccagcgcgac ctcgttcggc acttccttct cggcgagccc cgcgatggcc
    13081 ttcacgcagg cggctttcat cgcgtcgttg atcgtgctgg cgccgcagtc gagcgcgccg
    13141 cggaagatga acgggaagca cagcacgttg ttgacctggt tcgggtagtc cgaacggccg
    13201 gtcgcgagga tcgcgtcggg gcgtaccgcg agcgcctcct cgggcatgat ttccggcgtg
    13261 gggttcgcca tcgccaggat cagcgggcgc tcggccattt tcttcaccgc ggcggccttc
    13321 agcacgccgg ggccggacag gcccatgaag atgtcggcgc cgtccatcgc gtcatcgagc
    13381 gtgcgcagcg aggtgtcgcg cgcgtacttc tgcttccacg ggttcatgcc ctcggtgcgg
    13441 ccggtgtaga ccacgccgga gcgatcgacc agcgtcacgt gggccgggtc gaggcccatg
    13501 gtgaccagca ggtcgacgca ggcgatgctc gccgcgccgc tgcccgagac caccatcttc
    13561 acgtcctcga tcttcttgcc gacgatgcgc agggcgttgt agaccgccgc tcccgccacg
    13621 atcgcggtgc cgtgctggtc gtcgtggaac accgggatct tcatgcgctc gcgcagtttc
    13681 tgctcgacca ggaagcactc gggggccttg atgtcctcga ggttgatgcc gccgaaggtt
    13741 ggttccagcg aggcgatgat atcgacgagc ttgtcgacgt tcttctcgtc gatctcgatg
    13801 tcgaagacgt cgatgttcgc gaacttcttg aacagtac
//
LOCUS       DAORUQ010000082        13758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4335_length_13758_cov_5.754871, whole genome shotgun sequence.
ACCESSION   DAORUQ010000082 DAORUQ010000000
VERSION     DAORUQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4335_length_13758_cov_5.754871"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..700)
                     /locus_tag="PLW13_08725"
     CDS             complement(224..700)
                     /locus_tag="PLW13_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQY70498.1"
                     /translation="MPMLENLTFDEITVGQAASYARTVTEDDLLLFAAVSGDVNPVHL
                     DEAFAAGTQFKGRIAHGMYTGGLISAVIAMTLPGPGTIYLGQDLRFERPVRIGDTITV
                     QLTVLEKSDEKKLVKLQTVASNQNGKVVVSGTASVMAPSQKLRIEQPAAPKVSISR"
     gene            833..2500
                     /locus_tag="PLW13_08730"
     CDS             833..2500
                     /locus_tag="PLW13_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY70499.1"
                     /translation="MHAMELIERARAAAGMPQHVGLLEYDSVQAVLAAAVARFPEKPA
                     LSSLGHTLTFRELDRLTAAFASWLQHHTDLQPGERIAIQMPNLVQYPVVCLGALRAGL
                     IVVNTNPLYTAREMEHQFQDSGATALVLLANMAANAAPVLPHTGIRHVVVTELADLHP
                     FPRRLVINAGARYLKKLVPPYHIPGAVGLREALARGARQAHHEAAPAPQDVAVLQYTG
                     GTTGVSKGAMLSHGNIVANMQQAGVLFSTYDFREGEETLLLPLPLYHIYAFNACLGML
                     ARGAHTVLLPNPRDLQSLVAAFRDSRCSGFAGLNTLFVALCNDAGFRALDFSRLKVTM
                     SGGMALTMAAARRWKEVTGVEIVEGYGMSETSPVISLNPVNANHLGTIGIAVPSTEVR
                     ILDDDGNEVEAGEPGELVVRGPQVMLGYWNQAVETAKVLSPEGWLHTGDVAVVTPQGY
                     LKIVDRKKDMVVVSGFNVYPNEIEEVVVAHPDVLECAVVGIPDERTGEALRVFVVPRH
                     PGLTEKALRDFCRENLTGYKMPREFVIRTELPKSNVGKILRRELRGQ"
     gene            2522..6448
                     /gene="hrpA"
                     /locus_tag="PLW13_08735"
     CDS             2522..6448
                     /gene="hrpA"
                     /locus_tag="PLW13_08735"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HQY70500.1"
                     /translation="MVAGPPRAAGLPPALKELQSRLAEGMSGDAARLNRQLAQLADLV
                     RRGKPHDRLLAGIEAAIAESIARVARRRSLLPQAINYPEELPVCGARERILEAIAGHQ
                     VVILAGDTGSGKTTQLPKLCLELGRGVRGMIGHTQPRRIAAQAVASRIAEELGPAGAA
                     TVGWQVRFTDRSSPDTLVKLMTDGILLAEIQHDRRLERYDTIIIDEAHERSLNIDFLL
                     GYLKRLLPERPDLKLVITSATIDVERFARHFDGAPVIQVEGRTYPVEVWYRQPPEEEP
                     DLTRAIVAAIEELIAHERGTPHAGRGDILVFHAGERDIRETALALRKSDIAHAEVLPL
                     YSRLSQAEQASVFRPSARAGRRIVLATNVAETSLTVPGIRYVIDPGLARISRYSVRSK
                     VQRLPIEPIARASAEQRKGRCGRISEGICVRLYSEEDFAARPAFTDPEILRTGLASVI
                     LQMHALGLGEVGRFPFLDPPEERQVNDGIRLLEELGALDGKRLSAIGRQLARLPVDPR
                     IGRMLLAAAGLGALAEMLVVASFLSVQDPRERPVEKQQAADESHRRFACATSDFVSVL
                     QLWAYAEDKRQELSVSAWRRLCQREFLSFMRLREWRDIHYQLRLISRELGLRENHEPA
                     SEETLHRAVLAGMLTHIGTRTEERDYEGVRARRFNLHPGSFVFRKPPKWVVAAELVET
                     TRLYARSVAAIEPEWVLPWAEHLLKREHHSPHWSARAGKVMASERIRLFGLVLSDKKS
                     VDFGRIDPVEARRLFIQSALVEGDYQSKQKFWAHNRALLAEVEALEAKGRRRDLLVED
                     RVIHDFYDERLPSEVHDHPSLEKWLRAVQAREPEALCMQRASLLRKLDDGVSGAQFPD
                     ALVWQDLRVPLAYRYEPGHPADGVTAVLPLALLDHFPRHLPEWLVPGLLREKLIALLK
                     TLPKQYRKALVPVPDTVDALLPLLKPGDTPLLLALGEALRQGRGLAIPREAWQEEALD
                     AFYRMNLRVLDTAGAIVAEGRDLAAIRLQLAPQVRAEVQRAAPRQHARSGITRWDFGA
                     LPAVVRVSGKALDVPGYPAVRDCGETVATETFSDREEADAIHRAGVRRLLLLDCAQAA
                     AYLRRNLLKDARTGLRWGRGFDRAALVEDILLGAIEGCLPEDAALPREQADFAKLSER
                     CRREITPRAQQIEQLLGRMAEQLHAINGQRAILGKPAFAQAKRDAEAQLEALLAPGFL
                     YRTPARWLERLPVYLKALALRLEKLALRLPRDQEAVRELEVLGKRHADYLAAHPGSAA
                     AAVRVQEYRWMLEEYRVSVFAQQLGTVAPVSPKRLEKLWEELLAGS"
     gene            6536..7441
                     /locus_tag="PLW13_08740"
     CDS             6536..7441
                     /locus_tag="PLW13_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY70501.1"
                     /translation="MAIVVKQESLMVPVTGKVQLHLRRLWTGDKGAGQPVLMFHGAAE
                     DGHIFYSHSGLGLACFLARKGCEVFVPDMRGKGKSWPAINQHSDFGFHEIVTEDIPAL
                     AATVKRLGGGREQTWITHSLGGPLVAAALARLGPEPLGVRQLAHFAARRQIRTTGLRR
                     RLMVDLLWNRIGRLAVSFEGYLPATALGLGTARESRRCFLDGISWSTREQWIDAEDGF
                     DYGATLARGRWLRSLYFASATDRAFGSPDDVRDFMHELGQHDGRMLVLGREAGNLRDY
                     SHTGMLTHPEAEQDHFPQLLDWMRE"
     gene            7573..7935
                     /locus_tag="PLW13_08745"
     CDS             7573..7935
                     /locus_tag="PLW13_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70502.1"
                     /translation="MKTNSFKPLAAALGASMLATALAPAVSATENPFALNELKSGYQL
                     AQAETTEGTETKPAEGKCGEGKCGGEKTGTEGKCGGDKSGAEGKCGAGKAGEGKCGGA
                     AKANEGKCCAEGKCGGAA"
     gene            7953..8819
                     /locus_tag="PLW13_08750"
     CDS             7953..8819
                     /locus_tag="PLW13_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQY70503.1"
                     /translation="MQALPFDGAGLGLRRSLMGPLAALEAGQVDFLEVAPENWIGVGG
                     RLGRAFGALAERQPVLLHGLSLDIGGFAPLDTGLLAKVRDFKRAHRCPIYTEHLSYCA
                     ADGHLYDLLPIPFTEEAVRHVAARVRQVQDLLGERIALENASYYAAPAQQMGEAEFIR
                     AVLEEADCGLLLDVNNIYVNSINHRYDAREFLAALPGERAVCVHVAGHYVEAEDLRVD
                     SHGAAVIDPVWELLAEAYRLFGVLPTLVERDFNIPPLPVLLAEVARVREIQREQLAAA
                     HAMAARVAGARA"
     gene            8816..9586
                     /locus_tag="PLW13_08755"
     CDS             8816..9586
                     /locus_tag="PLW13_08755"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HQY70504.1"
                     /translation="MIGAHERAQRAFAAYVREPLGQPLPEGIEPRRMKIYAELVYNAF
                     EGFVAGGFPVLKSLLDPERWQALVRDFVAAHECRTPFFLQIGEEFLAFLQTGAAQRHA
                     LPGFALELAHYEWVELALDVSEQEVDAPGIDRAGALESGVPVLSALAWPLSYAWPVHR
                     ICAAFQPEACDAQPNCLVVYRDRADAVKFLETNPATLRLLGLVRDERLTGRELAARLA
                     VELGQPGVAAIEGAALDTLERLREMDIVAGVRLAATGR"
     gene            9686..10432
                     /locus_tag="PLW13_08760"
     CDS             9686..10432
                     /locus_tag="PLW13_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HQY70505.1"
                     /translation="MNALSRATIALLLSALLLGSTGARAAGEQADPWEGFNRRVFVFN
                     EFLDRNLLKPVAKGYQRITPEFVSNGVSNVFSNLGEVPSFVNHVLQGRLTEAGQDGGR
                     FAVNTTLGLVGLFDVASRMGIEQKNTDLGITLGRWGLRSGPYLVLPLLGPSTVRDGVG
                     RGGDHFLAPLNYVEDDTLRWSLRSLDLVDTRADLLSVEELITGDRYVFMRNLYIKRRA
                     FLLNGGEPPPAEFEDEFDDDLGDEFDGEEL"
     gene            10541..10705
                     /locus_tag="PLW13_08765"
     CDS             10541..10705
                     /locus_tag="PLW13_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70506.1"
                     /translation="MNPSTGTAMFTPDPRRSRGNRGDAAKKAVAAGQGAWFGRLESWL
                     RRSRRPRSAD"
     gene            complement(10709..11176)
                     /locus_tag="PLW13_08770"
     CDS             complement(10709..11176)
                     /locus_tag="PLW13_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HQY70507.1"
                     /translation="MLTKLKEFLAARLGADTPPVHSAHGRHLAAAALLIEVARADYGF
                     DPREQLALQEALRRSFDLSDAEIETLLALADAEAREATSSYAFTRLINEEYSDEEKSD
                     LLRAMWSVAFADGAVDKYEEHLIRKIAGLIYVPHQEFIRTRLDAGAQFEQNRG"
     gene            complement(11170..12216)
                     /gene="dusA"
                     /locus_tag="PLW13_08775"
     CDS             complement(11170..12216)
                     /gene="dusA"
                     /locus_tag="PLW13_08775"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HQY70508.1"
                     /translation="MTDISHICQPPASHRFCVAPMMEWTDRHCRYFLRLLSRHALLYT
                     EMVTSAAIVHGDRARLLDFDAGEHPVALQLGGSDPRELAESARIVEQWGYVEINLNVG
                     CPSDRVQSGRFGACLMAEPALVAECVAAMRAAVRVPVTVKCRIGIDERDSWEELHEFV
                     RLVSEAGSRTVIVHARKAWLSGLSPKENREIPPLVYPTVYCIKQDFPALDIVINGGID
                     SLDACREHLRHVDGVMLGRAAYHNPWLLAQVDAELFGDAHPVPSRHEALEAFLPYVER
                     QLRAGRRLCQISRHVLGLFQGCPGGRRFRRHIAEQGYKDDAGIEVLRDAAAWVPRDMG
                     APPARATIGGYAPC"
     gene            12519..13733
                     /locus_tag="PLW13_08780"
     CDS             12519..13733
                     /locus_tag="PLW13_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY70509.1"
                     /translation="MKSDIQPAIDRGESISLMEPLLLSSDSRHRGVLTDLAVELAART
                     TGFRRSLPASLVGALAELVRAMNCYYSNLIEGHNTHPVDIERALKGDYSDDPRKRNLQ
                     LEATAHIAVQRWIDTGGLQESAVSGASLAEIHRRFCEQLPDELLWVEDPLTKERIRVI
                     PGEFRRRDVAVGQHIPVSAGAVPRFLERFSEAYSPLGKTETILSLAAAHHRLLWIHPF
                     LDGNGRVARLVSHATLLTALDTGAIWSVARGLARNVDTYKRHLAACDLTRRNDLDGRG
                     HLSEESLAAFAEFFLRTCLDQVEFMEGLMRPDALRARILLWAEEEIRLGELPARANTL
                     LDALLYRGEIPRGDAQTIIGTGERQARRVVAALIERGAITSASPKLPLHLAFPATLVS
                     RWMPDLFPERTG"
BASE COUNT         2111 a         4659 c         4792 g         2196 t
ORIGIN      
        1 gccaggacgg cgacaggtag cctacaggga tgtattcacg gcgtgtccgc ggcactgatg
       61 ggtgctcgag cgccgatggc tcgcgatacg cgggattgtc aggccgctat ccagctcacg
      121 gatggtttta caacaccctc ttcaaccgga caattgtccg gataaatccg gacctgcgcc
      181 agacctgcgc cggtggccag gcgcagcagt gagcgacgcg ccgtcagcgc gaaatgctga
      241 ccttcggtgc cgcgggctgc tcgatgcgca gcttctgcga cggcgccatc acgctggcgg
      301 taccgctcac gaccaccttc ccgttctggt tgctggccac ggtctgcagc ttcaccagct
      361 tcttttcgtc ggacttctcg agcaccgtga gctgcaccgt gatggtgtcg ccgatgcgta
      421 ccgggcgctc gaagcgcagg tcctggccga ggtagatcgt gcccggaccc ggcagcgtca
      481 tcgcgatcac ggcggagatc agcccgccgg tgtacatgcc gtgcgcgatg cggcccttga
      541 actgggtgcc cgcggcgaac gcctcgtcga gatgcaccgg attgacgtcg ccggagacgg
      601 ccgcgaacag cagcaggtcg tcctccgtga cggtgcgcgc gtagctcgcg gcctggccga
      661 cggtgatctc gtcgaaggtc aggttttcca gcatcggcat tgtcggcgct ccggctcgtt
      721 tcggggaggc gcggattatt gcagcgcact cctgcgattg ccagccgcga agcatcttcg
      781 ctacagtctt caccctcagc ttctctgacc cgcccggggg cgacgaacgg caatgcacgc
      841 aatggaactg atcgagcggg cgagggccgc cgcgggcatg ccgcagcacg tcgggctgct
      901 ggagtacgac agcgtgcagg cggtgctcgc ggccgcggtg gcgcgctttc ccgagaagcc
      961 cgcgctgagc agcctgggcc acacgctcac gttccgcgag ctcgaccggc tgaccgcggc
     1021 gttcgcgtcg tggctgcagc accacacgga cctgcagccc ggggagcgca tcgcgatcca
     1081 gatgccgaac ctggtgcagt acccggtggt gtgcctgggg gcgttgcgcg ccgggctgat
     1141 cgtcgtgaac accaacccgc tctacacggc gcgcgagatg gagcaccagt tccaggactc
     1201 gggcgcgacg gcgctggtgc tgctggcgaa catggcggcg aacgccgcgc cggtgctccc
     1261 gcataccggc atccgccacg tcgtggtcac ggagcttgcg gacctgcacc cgttcccgcg
     1321 ccgcctggtg atcaacgccg gggcgcgcta cctgaagaag ctggtgccgc cgtaccacat
     1381 accgggcgcg gtggggctgc gcgaggcgct cgcacggggc gcgcggcagg cgcatcacga
     1441 ggcggcaccg gcgccgcaag acgtggcggt gctgcaatac accggcggca ccacgggcgt
     1501 ctcgaagggc gcgatgctct cgcacggcaa catcgtcgcc aacatgcagc aggccggcgt
     1561 gctgttctcg acctacgatt tccgcgaggg cgaggagacg ctgctgctgc cgctgccgct
     1621 gtatcacatc tatgcgttca atgcctgcct cggcatgctc gcgcgcggtg cgcacacggt
     1681 gctgctgccc aatccgcgcg acctgcagag cctggtggcc gcgttccgcg actcgcgctg
     1741 ctcgggcttc gccggcctga acacgctgtt cgtggcgctg tgcaacgacg cgggtttccg
     1801 cgcgctcgat ttctcgcggc tcaaggtcac gatgtccggt ggcatggcgc tcacgatggc
     1861 cgccgcgcgt cgctggaagg aagtcaccgg tgtggagatc gtcgagggct acggcatgag
     1921 cgagacctcg ccggtgatct cgctgaaccc ggtgaacgcg aatcacctcg gcaccatcgg
     1981 catcgcggtg ccctcgaccg aggtgcgcat tctcgacgac gacggcaacg aggtggaggc
     2041 aggcgagccc ggcgagctgg tggtgcgcgg cccgcaggtg atgctcggct actggaacca
     2101 ggccgtggag accgcgaagg tgctctcgcc cgagggttgg ctgcacaccg gcgacgtggc
     2161 cgtggtgacg ccgcagggtt acctgaagat cgtcgaccgc aagaaggaca tggtcgtggt
     2221 gtccgggttc aatgtgtacc ccaacgagat cgaggaggtc gtggtcgcgc atccggacgt
     2281 gctggagtgc gcggtggtcg gcattcccga tgagcgcacc ggcgaggcgc tgcgcgtgtt
     2341 cgtggtgccg cgccatcccg gcctgaccga gaaggcgctg cgcgatttct gccgtgagaa
     2401 cctcaccggc tacaagatgc cgcgcgagtt cgtgatccgc accgaactgc cgaagtccaa
     2461 cgtcggcaag atcctgcgcc gcgagctgcg cgggcaatga gcggccggag gcgctgaacg
     2521 catggtggcc gggccgccgc gcgcggccgg gttgccgccc gcgttgaagg aactgcagtc
     2581 gcgcctggcc gagggcatga gcggtgatgc cgcgcgcctg aaccgccagc tggcgcaact
     2641 cgcggacctg gtgcgccgcg gcaagccgca cgaccggctg ctggcgggca tcgaggccgc
     2701 gatcgccgag tcgatcgcgc gcgtggcgcg gcgacgttcg ctgttgccgc aggcgataaa
     2761 ttaccccgag gaactgccgg tctgcggcgc gcgcgaacgc atcctggagg ccattgccgg
     2821 acaccaggtg gtgatcctcg ccggcgacac cggctcgggc aagaccacgc agctgcccaa
     2881 gttgtgcctg gagctcgggc gcggcgtgcg cggcatgatc ggccacacgc agccgcggcg
     2941 catcgccgcg caggccgtcg cctcgcgcat cgccgaggag ctcggtcccg cgggcgcggc
     3001 gacggtcggc tggcaggtgc gcttcaccga tcgctcctcg cccgacacgc tggtcaagct
     3061 gatgaccgac ggcatcctgc tcgcggagat ccagcacgac cgccgcctcg agcgctacga
     3121 caccatcatc atcgacgagg cgcacgagcg cagcctcaac atcgacttcc tgctcggcta
     3181 cctgaagcgc ctgctgcccg agcgacccga tctcaagctc gtgatcacct cggcgaccat
     3241 cgacgtcgag cgcttcgcgc gccacttcga cggcgcgccg gtgatccagg tcgaggggcg
     3301 cacctacccg gtcgaggtct ggtatcgcca gccgcccgag gaggagcccg acctgacgcg
     3361 cgcgatcgtc gccgcgatcg aggagctcat cgcgcacgag cgcggcacgc cgcatgccgg
     3421 gcgcggcgac atcctggtgt tccacgccgg tgagcgcgac atccgcgaga cggcgctggc
     3481 gctgcgcaaa tccgatatcg cgcacgccga ggtgctgccg ctctactcgc ggctgtcgca
     3541 ggccgagcag gcgagcgtgt tcaggccctc ggcgcgcgcc gggcggcgca tcgtgctggc
     3601 gaccaacgtc gccgagacct cgctcacggt gccggggatc cgctacgtga tcgacccggg
     3661 gctggcgcgc atcagccgct acagcgtgcg ctcgaaggtg cagcgcctgc ccatcgagcc
     3721 catcgcccgc gccagcgccg agcagcgcaa gggccgctgc gggcgcatca gcgaaggcat
     3781 ctgcgtgcgg ctgtattccg aggaggattt cgcggcgcgc cccgcgttca ccgaccccga
     3841 gatcctgcgc accgggctcg cctcggtgat cctgcaaatg cacgcgctgg ggctcggcga
     3901 ggtggggcgc tttccgttcc tcgacccgcc cgaggagcgc caggtcaacg acggcatccg
     3961 cctgctcgag gagctcggtg cgctggatgg caagcgcctg agcgcgatcg gccggcagct
     4021 cgcgcgcttg ccggtggacc cgcgcatcgg gcgcatgttg ctcgccgccg ccggcctcgg
     4081 cgcgctggcc gagatgctgg tggtcgcgag tttcctcagc gtgcaggacc cgcgcgagcg
     4141 cccggtggag aagcagcagg cggccgacga gagccaccgc cgcttcgcct gcgccacctc
     4201 ggatttcgtc tcggtgctgc agctctgggc ctatgccgag gacaagcgcc aggagctgtc
     4261 ggtgagcgcg tggcggcggc tgtgccagcg cgagttcctg tcgttcatgc gcctgcgcga
     4321 gtggcgcgac atccactacc agttgcgcct gatcagccgc gaactgggcc tgcgcgaaaa
     4381 ccacgagccg gccagcgagg aaacgctgca ccgcgcggtg ctcgccggca tgctgacgca
     4441 catcggcacg cgcaccgagg agcgcgacta cgagggcgtg cgcgcgcgtc gcttcaacct
     4501 gcatcccggc tcgttcgtgt tccgcaagcc gcccaaatgg gtggtggcgg cggaactggt
     4561 cgagaccacg cggctctacg cgcgcagcgt ggccgcgatc gagcccgagt gggtgctgcc
     4621 ctgggccgag cacctgctca agcgcgagca ccactcgccg cactggtcgg cgcgcgcggg
     4681 caaggtgatg gccagcgagc gcatccgcct gttcgggctg gtgctctcgg acaagaagag
     4741 cgtggacttc gggcgcatcg acccggtgga ggcgcggcgc ctgttcatcc agagcgcgct
     4801 ggtcgagggc gattaccaga gcaagcagaa attctgggcg cacaaccgcg cgctgctcgc
     4861 cgaggtcgag gcgctggagg cgaaggggcg ccggcgcgac ctgctggtcg aggatcgcgt
     4921 gatccatgac ttctacgacg agcgcctgcc gtccgaggtg cacgatcatc cctcgctgga
     4981 gaaatggctg cgcgcggtgc aggcgcgcga gcccgaggcg ctgtgcatgc agcgcgcgag
     5041 cctgctgcgc aagctcgatg atggtgtcag cggcgcacag ttccccgatg cgctggtgtg
     5101 gcaggacctg cgcgtgccgc tggcgtatcg ctacgagccg gggcatccgg ccgacggtgt
     5161 gaccgcggtg ctgccgctgg cgctgctcga tcacttcccg cgccatctgc cggagtggct
     5221 ggtgccgggg ctgctgcgcg agaagctgat cgcgctgctc aagacgctgc ccaagcagta
     5281 tcgcaaggcg ctggtgccgg tgcccgatac cgtggacgcg ctgctgcccc tgctgaagcc
     5341 cggggacaca ccgctgctcc tcgcgctcgg cgaggcattg cgccagggcc gcgggctcgc
     5401 gatcccgcgc gaggcctggc aggaggaggc gctcgatgcc ttctaccgca tgaacctgcg
     5461 cgtgctcgat accgcgggcg cgatcgtggc agaggggcgc gatctcgccg cgatccgcct
     5521 gcagctcgcg ccgcaggtgc gcgccgaggt gcagcgtgcc gcgccccggc agcacgcgcg
     5581 cagcggcatc acgcgctggg acttcggcgc gctgcccgcc gtggtgcgcg tctcgggcaa
     5641 ggcgctcgac gtgccgggtt accccgcggt gcgcgactgc ggcgagacgg tggcgaccga
     5701 gactttctcc gatcgcgagg aggctgacgc gatccaccgt gccggcgtgc gccgcctgct
     5761 cctgctcgac tgcgcgcagg ccgcggccta cctgcgccgc aacctgctga aggatgcgcg
     5821 caccgggctg cgctggggcc gcggtttcga ccgcgcggca ctggtcgagg acatcctgct
     5881 cggcgccatc gagggttgtc tgcccgaaga cgccgcgctg ccgcgcgagc aggcggattt
     5941 cgcgaagctc tcggagcgct gccggcgcga gatcacgccg cgcgcgcagc agatcgaaca
     6001 gctgctgggg cgcatggccg agcagttgca cgcgatcaac ggccagcgcg cgatcctcgg
     6061 caagcctgcc ttcgcccagg ccaagcgcga tgccgaggcg cagctcgagg ccctgctcgc
     6121 gcccggtttc ctctatcgca cccccgcgcg ctggctggag cgcctgccgg tgtacctgaa
     6181 ggcactggcg ctacgcctcg agaagctcgc gctgcggctg ccgcgcgacc aggaggcggt
     6241 gcgcgagctc gaggtgctgg gcaagcgcca cgccgattac ctggctgcgc accccggttc
     6301 ggccgctgcc gccgtgcgcg tgcaggagta ccgctggatg ctcgaggaat accgcgtgtc
     6361 ggtgttcgcg cagcagctgg gcaccgtggc gccggtatcg ccgaagcggc tggagaaact
     6421 gtgggaggaa ctgctggcag gcagctgatg cgagcgccat tgccgggcac gcgatcggcg
     6481 ggggcgaggg ctatcggcta cacttgcggc tcgttcccgc agccgaatcc agcgcatggc
     6541 aatcgtcgtc aaacaggaat cgctcatggt gcccgtgacc ggcaaggtcc agctgcacct
     6601 gcgccgcctg tggacaggag acaagggcgc cggccagccg gtgctgatgt tccacggcgc
     6661 cgccgaggac gggcacatct tctactcgca ctcgggcctg gggctggcgt gcttcctcgc
     6721 gcgcaagggc tgcgaggtct tcgtgcccga tatgcgcggc aagggcaaga gctggcccgc
     6781 gatcaaccag cacagcgatt tcggcttcca cgagatcgtg accgaggaca tccccgcgct
     6841 ggccgccacc gtgaagcgcc tgggcggcgg gcgcgaacag acctggatca cgcactcgct
     6901 gggcggtccg ctggtggccg cggcgctggc gcgcctcgga cccgagccgc tgggcgtgcg
     6961 ccagctggcg catttcgccg ccaggcgaca gatccgcacc acgggtttgc gtcgccgcct
     7021 gatggtcgac ctgctgtgga accgcatcgg ccggctggcg gtgtcgttcg agggctacct
     7081 gccggcgacg gcgctgggcc tgggtaccgc gcgcgaatcg aggcgctgct tcctcgacgg
     7141 catcagctgg tccacgcgcg agcagtggat cgatgccgag gacggcttcg actatggcgc
     7201 gacgctggcg cgcgggcgct ggctgcgcag cctgtatttc gcctcggcca ccgaccgcgc
     7261 cttcggcagt cccgacgacg tgcgcgactt catgcacgaa ctcggacagc acgacgggcg
     7321 catgcttgtg ctggggcgcg aggcgggcaa tctgcgcgac tattcgcaca ccggcatgct
     7381 gacgcacccc gaggccgagc aggaccactt cccgcaactg ctcgactgga tgcgcgagta
     7441 acgcaggtcg ggcttcagcc cgacaagcgt tcgcccgccc catctggacc tcacccgccc
     7501 ctcggggttt aatgcctgcg gtgctcacgc gccctccgtt acgacccaca agcattccga
     7561 ggagagatcc cgatgaaaac caacagcttc aagccgctgg ccgcggccct tggcgcctcc
     7621 atgctcgcca ccgcgctggc acccgccgtt tcggccacgg agaacccctt tgcgctgaac
     7681 gaactgaagt ccggctacca gctggcgcag gccgagacca ccgagggcac ggaaacgaag
     7741 cccgccgaag gcaagtgcgg cgagggcaag tgcggcggtg aaaagaccgg caccgagggc
     7801 aagtgcggcg gcgacaagag cggcgccgag ggcaagtgcg gcgccgggaa ggccggcgag
     7861 ggcaaatgcg gcggtgccgc gaaggccaac gagggcaagt gctgcgccga aggcaagtgc
     7921 ggcggcgctg cctgagccgc gccgtccacg cgatgcaggc gctgccattc gacggggcag
     7981 ggctcgggtt gcggcggtcc ctgatggggc cgctcgcggc gctcgaggcc ggccaggtcg
     8041 attttctcga ggtcgcgccc gagaactgga tcggtgtcgg cgggcggctg gggcgcgcgt
     8101 tcggcgcgct cgccgaacgc cagccggtct tgctgcacgg tctgtcgctg gacatcggcg
     8161 gtttcgcgcc gctcgatacc gggttgctgg caaaggtgcg cgatttcaag cgcgcgcacc
     8221 gctgcccgat ctacaccgag cacctcagct actgcgccgc ggacggtcac ctctacgacc
     8281 tgctgccgat cccgttcacc gaggaggccg tgcgccacgt cgccgcgcgc gtgcgccagg
     8341 tgcaggacct gctcggcgag cgcatcgcgc tggagaacgc ctcgtactac gccgcgcccg
     8401 cgcagcagat gggcgaggcc gagttcatcc gcgcggtgct cgaggaggcc gactgcgggc
     8461 tgctgctcga cgtgaacaac atctacgtca acagcatcaa ccaccgctac gacgcgcgcg
     8521 agttcctggc cgcgctgccc ggtgagcgcg cggtctgtgt ccacgtggcc gggcattacg
     8581 tcgaggccga ggacctgcgc gtcgattcgc atggcgccgc ggtgatcgac ccggtctggg
     8641 agctgctggc cgaggcctac cggctcttcg gcgtgctccc gacgctggtg gagcgcgact
     8701 tcaacatccc gccgctgccg gtgctgctcg cagaggtcgc gcgcgtgcgc gagatccagc
     8761 gggagcagct cgctgccgcg cacgcgatgg cagcgcgcgt tgcgggagcg cgcgcatgat
     8821 cggcgcacac gagcgcgccc agcgcgcctt cgcggcgtac gtgcgcgagc cgctggggca
     8881 gcccctgccc gagggtatcg agccgcggcg catgaagatc tacgccgagc tggtctacaa
     8941 cgccttcgag ggtttcgtgg ccggcggctt tccggtgctg aagagcctgc tcgatcccga
     9001 acgctggcag gcgctggtgc gtgacttcgt tgccgcgcac gagtgccgca cgccgttctt
     9061 cctgcagatc ggcgaggagt tcctcgcctt cctgcagacg ggcgccgcgc aacggcacgc
     9121 gctgcccggc tttgcgctgg agctcgcgca ctacgagtgg gtggagctcg cgctggatgt
     9181 gtcggagcag gaagtggatg cacccggcat cgatcgcgcc ggcgcgctcg aatcgggcgt
     9241 cccggtactg agcgcgctgg cctggccgct gtcctatgcc tggccggtgc atcgcatctg
     9301 tgcggctttc cagcccgagg catgcgatgc gcagccgaac tgcctcgttg tctaccgcga
     9361 tcgcgccgat gcggtgaagt ttctcgagac caatcccgcg acgctgcgcc tgctgggact
     9421 ggtccgcgac gagcgcctga cgggtcgcga actggcggcg cgactggcag tggaactggg
     9481 tcagccgggc gtggccgcta tcgaaggcgc cgcgctcgac acgctggagc gtctgcgcga
     9541 gatggatatc gtggcagggg tgcgcctggc ggcaacgggc cggtgatctg caggcggccc
     9601 gccgagcgta accgcatatg tcgcgaactg gcccaagcga atgcgtatgc taagctggca
     9661 cattgatttt gcacggtatt ccccgatgaa cgctctctct cgcgccacca tcgcgctgct
     9721 gctctccgcc ctgctgctgg gcagcacggg cgcgcgcgcg gcaggcgaac aagccgatcc
     9781 ctgggagggt ttcaaccggc gcgtgttcgt attcaatgag ttcctcgatc gcaacttgct
     9841 caagcccgtg gccaaggggt atcagaggat caccccggag ttcgtcagca acggtgtcag
     9901 caacgtgttc agcaacctgg gcgaggtgcc gagcttcgtc aatcacgtgc tgcagggacg
     9961 ccttacggag gccggccagg atggcggacg tttcgcggtc aacaccacgc tcgggctggt
    10021 cggcctgttc gatgtcgcga gcaggatggg catcgagcag aagaacacgg acctgggcat
    10081 cacgctggga cgctggggcc tgcgctccgg accctacctg gtgctgccgc tcctcggtcc
    10141 gagcacggtg cgcgacggcg tgggccgggg cggcgaccac ttcctggcgc cgctcaacta
    10201 tgtcgaggac gacaccctgc gctggtcgct tcgcagcctc gatctggtcg acacgcgcgc
    10261 ggacctgctg agcgtcgagg aactgatcac gggcgaccgc tacgtgttca tgcgcaacct
    10321 ctacatcaag cggcgcgcct tcctgctcaa cggaggcgag ccgccaccgg cagagttcga
    10381 ggacgagttc gacgacgatc tgggcgatga attcgacggc gaagaactct gattctcggc
    10441 ccgtttgcca aatagttctc gaattccccg ttccccgggc gcgacccgtt ccagccggtg
    10501 ctataggaac ctgaccgttc ccgtgatcga ggcctgcgcg atgaacccct ccaccggcac
    10561 cgcgatgttc actcccgacc cgcgccgcag ccgcggcaac cgtggcgatg cggcgaagaa
    10621 agcggtcgct gcgggacagg gtgcgtggtt cgggcgcctg gaatcgtggt tgcgccgcag
    10681 ccggcgcccg cgcagcgccg attgagcctc agccgcggtt ctgttcgaat tgcgcaccgg
    10741 cgtcgagccg ggtgcggatg aattcctggt gcgggacgta gatcagtccc gcgatctttc
    10801 ggatcaggtg ctcctcgtac ttgtccaccg cgccgtcggc gaaagccacc gaccacatcg
    10861 cgcgcagcag atccgacttc tcctcgtccg aatactcctc gtttatcagg cgcgtgaacg
    10921 catagctgga cgtggcttcg cgcgcctcgg cgtcggccag cgcgagcagt gtctcgattt
    10981 ccgcgtcgct cagatcgaag ctgcggcgca gcgcctcctg cagcgccagc tgctcgcgcg
    11041 gatcgaaacc gtagtcggcg cgcgcgacct cgatcagcag cgcagcggcg gcgaggtggc
    11101 gcccgtgcgc gctgtgcacg ggcggcgtgt ccgcgccgag gcgggcggcg aggaattcct
    11161 tcagcttcgt cagcacggcg catagcctcc gatggtggcg cgagcgggcg gcgcgcccat
    11221 gtcgcgggga acccaggccg cggcgtcgcg cagcacctcg atgcccgcat cgtccttgta
    11281 gccctgctcg gcaatgtggc ggcgaaagcg cctgccgccc gggcagccct ggaacaggcc
    11341 gagcacgtgg cggctgatct ggcacaggcg ccggcccgcg cgcagttgcc gttcgacgta
    11401 gggcaggaag gcttccagcg cctcgtgccg cgagggcacc ggatgcgcgt cgccgaacag
    11461 ctcggcgtcg acctgcgcca gcagccaggg attgtggtag gcggcgcgcc ctagcatcac
    11521 gccatcgacg tggcgaaggt gctcgcggca ggcatccagc gaatcgatgc cgccgttgat
    11581 cacgatgtcc agcgccggga aatcctgctt gatgcaatag accgtcgggt agaccagcgg
    11641 cgggatctcg cggttctcct tcggcgacag cccgctcagc caggccttgc gcgcgtgcac
    11701 gatcacggtc cgagagccgg cctcgctcac caggcgcacg aactcgtgca gttcttccca
    11761 ggagtcgcgc tcgtcgatac cgatgcggca cttcacggtc accggcacgc gcacggccgc
    11821 gcgcatcgcg gcgacgcact ccgcgacgag cgccggctcg gccatcaggc aggcgccgaa
    11881 gcggcccgac tgcacgcggt ccgaggggca gcccacattc aggttgatct ccacatagcc
    11941 ccattgctcg acgatgcgcg cactctcggc gagctcgcgc ggatccgatc cgccgagctg
    12001 cagcgcaaca gggtgctcgc cggcgtcgaa atcgagcagc cgcgcgcggt cgccgtggac
    12061 gatcgccgca ctggtgacca tctcggtgta cagcaacgcg tgacgcgaca gcaggcgcag
    12121 gaagtacctg cagtggcggt cggtccactc catcatcggc gccacgcaga agcggtgcga
    12181 cgcaggcggc tggcagatat gcgaaatgtc agtcaattgg agggaaatcc gatcgccttt
    12241 ttcagcttca ttctccaggg ttttgcctgt tgtgtcaggc aggttttact gaaagtttgc
    12301 ggcgttgaca gggtgggtgg agcgctgctg cgggatgcgg tcaagccatg acgcggaaat
    12361 cttgcgctcg tacgcgtcct cgatggatgt tgcgctggga tgcgcgtgct ggtttcattc
    12421 catccggcga tttatccgga ctttcttccg gcctcaaatc tgtcgatttc tctctaacta
    12481 aatgataacg ttgacatgat tttctattga atccggacat gaaatcggac atacaaccgg
    12541 ccatcgatcg cggcgagagc atctccctga tggagccgct tctcctcagt agcgactcgc
    12601 gacaccgcgg tgtgttgacc gatctggccg tggaactggc cgcacgaacc acagggtttc
    12661 ggcgatcgct ccccgcgagc ctcgtgggcg cgcttgcgga actcgtgcgc gcaatgaact
    12721 gctactacag taacctgatc gaggggcaca acacgcaccc tgtcgatatc gagcgcgcgc
    12781 tcaaaggtga ctacagcgat gacccccgga agcgcaatct tcagcttgaa gccacggcgc
    12841 atatagcggt ccagcgctgg atcgacacgg gtgggctgca ggagagcgcg gtctcgggcg
    12901 cctcgctcgc agagatccat cgccgcttct gcgagcagtt gccggatgag ctcctgtggg
    12961 tggaggaccc tctcacgaag gagaggatcc gggtaatccc cggcgagttc cgccggcgtg
    13021 atgttgcagt cggccagcac atccccgtca gtgcgggcgc cgttcctcga ttcctcgagc
    13081 gattcagcga ggcatactcc ccgcttggca agaccgagac gattctatcg ctcgctgcgg
    13141 cgcatcaccg gctcctgtgg attcacccct tcctcgatgg caacgggcgc gttgcgcgtc
    13201 tcgtttccca cgcgaccttg ctcaccgcgc tcgacaccgg cgccatctgg tccgttgcgc
    13261 gcggactcgc acgcaacgtc gatacgtaca agcgccattt ggcggcgtgt gatctcacgc
    13321 ggcgcaacga tctcgatggc cgcgggcact tgagcgaaga gagccttgcc gcgtttgccg
    13381 aattcttcct ccgcacttgt ctcgaccagg tggagttcat ggaaggcctg atgcgtcccg
    13441 atgcgctgcg cgcgcgcatc ctgttgtggg ccgaagagga gatccggctt ggcgagttgc
    13501 cggcgagagc aaataccctg ctcgatgcgc tgctctatcg cggtgagatc ccgcgcggtg
    13561 atgcccagac gatcattgga accggcgagc ggcaggcaag gcgcgtcgtc gcggcgctca
    13621 ttgagcgcgg cgccatcacg tcggcgagcc cgaagctgcc tctccatctc gcgttccctg
    13681 caaccctggt gtcgcgctgg atgccggacc tctttcccga gcgtaccggc tgatcaagat
    13741 ccccgctcct gatcggcg
//
LOCUS       DAORUQ010000083        13703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4359_length_13703_cov_6.953107, whole genome shotgun sequence.
ACCESSION   DAORUQ010000083 DAORUQ010000000
VERSION     DAORUQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_13703_cov_6.953107"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="aspS"
                     /locus_tag="PLW13_08785"
     CDS             complement(<1..1075)
                     /gene="aspS"
                     /locus_tag="PLW13_08785"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQY70510.1"
                     /translation="MRTHYCGDLDTSHVDQEVTICGWADRRRDHGGVIFVDLRDREGV
                     VQVVFDPDCGEHFATADRVRSEYVLRVRGRVRARAEATVNPAMKTGRIEVYARELEIL
                     NVSDTPPFQLDEHANVGEETRLRYRYLDLRRPEMLNNLRLRSDISMAIRNFLAERGFI
                     DVETPMLTRATPEGARDYLVPSRTHPGRFFALPQSPQLFKQLLMVAGVDRYYQIVKCF
                     RDEDLRADRQPEFTQIDLEFAFADQAEITATAEAMVRGLFAQVLKHDLGEFPRLTWAE
                     AMARFGSDKPDLRIPLELVEVSDLMRAVDFKVFSAPANDPDGRVAALKVPGGGSLLSR
                     KQIDDYTRFVGIYGAKGLAYIKVN"
     gene            complement(1113..1394)
                     /locus_tag="PLW13_08790"
     CDS             complement(1113..1394)
                     /locus_tag="PLW13_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY70511.1"
                     /translation="MPIYEYQCQGCNRELEKLQKISDEPLTVCPECGEPQLKRKISAA
                     GFRLKGGGWYETDFKSGSKKNLAGDSGSSGGDSKPAASSPAPTSGAGGA"
     gene            1752..2177
                     /locus_tag="PLW13_08795"
     CDS             1752..2177
                     /locus_tag="PLW13_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQY70512.1"
                     /translation="MAADKKKAAKAAPKKAAAKAAPKKAAAKAAPKAAAKPTPVKESW
                     SKTQILNEIAERTQLSRKQVTAVMAELEGIMSRHIAKNGPGDFVLPGLLKITTVKKPA
                     TKARKGIDPFTKQERMFAAKPASVKVKIRPLKKLKDMAG"
     gene            2405..4129
                     /locus_tag="PLW13_08800"
     CDS             2405..4129
                     /locus_tag="PLW13_08800"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQY70513.1"
                     /translation="MRSSQLLIATLKQTPADAEVVSHQLMLRAGLIRRLASGLYTWLP
                     LGLRVLRKVETIVREEMDRSGAQEVLMPVVQPAELWQESGRWQQYGPELLRISDRHER
                     AFCLGPTHEEVITDLARNELRSYRQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLDAESLDQTYRGMHRAYCRIFDRIGLGYRPVIADTGSIGGSESHEFHVLADSGE
                     DAIAFSDASDYAANVEKAEALAPTAPRAHPAAARAMVATPGKHTIADVATFLGVPEAR
                     MVKTLVVNGSSEAAPLVALALRGDHQLNAVKAEAHPLVASPLCLASEAAITAALGCRP
                     GSIGPADLGIPVIADRSAAALADFVCGANQDGQHLTGVNWERDCNYAEVCDLRNVVDG
                     DPSPDGHGNIVVRRGIEVGHIFKLGQKYSAALGASVLDDAGAERTMTMGCYGIGVSRI
                     VAAAIEQNHDAAGIVWPDAIAPFTVVLLPLNMHKSALVRETAERLYAQLAAAGIDVLL
                     DDRDERPGVKFADMELIGIPHHVVIGERSLGAGMLEYKHRAGGEREEVPVGSIGDFLS
                     ARIKTATA"
     gene            4146..4298
                     /locus_tag="PLW13_08805"
     CDS             4146..4298
                     /locus_tag="PLW13_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70514.1"
                     /translation="MLNLIVLLAIAWLLWLIREELAQMSERQRSLKLTLERLAERLDT
                     SGKNET"
     gene            complement(4300..5265)
                     /gene="hemB"
                     /locus_tag="PLW13_08810"
     CDS             complement(4300..5265)
                     /gene="hemB"
                     /locus_tag="PLW13_08810"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQY70515.1"
                     /translation="MPFRFRRLRRTERLRSLVREHSLTSAQLILPVFVEEGVAAAVPI
                     ASMPGVVRHPEAALGEVVARARDSGIGAVMLFGVSHRKDACGSDTWNPDGLMARMIRS
                     AKQAAPDMLVISDNCFCEYTDHGHCGVVAGDQVQNDATLENLVRQALTAAHAGVDMIA
                     PSGMMDGMVAALRGGLDSAGFGQLPIMSYSTKFASAFYGPFRDAVDSSFKGSRSSYQL
                     DMANGREALAESVRDEQEGADILMVKPGLAYLDVLANIRAHCARPLAVYQVSGEYAMI
                     KAAVAAGVMEERPLVLETMMAFRRAGADMILSYYAEDVARWLRTG"
     gene            complement(5321..5587)
                     /locus_tag="PLW13_08815"
     CDS             complement(5321..5587)
                     /locus_tag="PLW13_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HQY70516.1"
                     /translation="MAIDRLRADLGRLREEIARADAGDRDALARLEELAGQVQRELDE
                     ENAVGDPAGLVDELEEAITGFEARHPNLTAIVNNMLVALGSMGV"
     gene            complement(5654..6778)
                     /locus_tag="PLW13_08820"
     CDS             complement(5654..6778)
                     /locus_tag="PLW13_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70517.1"
                     /translation="MITSFDDYCIHQTHEPVAQPSQSDRNFYDRYWFNGFDTGGAFLF
                     EIGFGLYPNRRVMDGHFSVVVDGVQHSFHGSRRAPADRAQTVVGPLAIEVQEPLRRVR
                     VRLVSNETGIECDLLFAARSVPVEEPKNLMFDDGRLIMHNSRFTQFGCWSGWFKVGGV
                     RHEVRAEQSLGARDKSWGVRPVGEPEGGAPGKLNGEPGVYWVWSPIDFGDVATQFNTF
                     EDRDGHPTQLGACMVPVYGAPELIPAEEPALQHMQTARHRIHWASGTRRAKGAEIVLV
                     EAAGVEHRITLEPVLTFQMKGIGYSHPSWGHGIWKGEEAIAGESWRLDEIDPLDYPNI
                     HVHQLVRARMGERVGLGTLETICFGRHAPSGFASFFDGAP"
     gene            complement(6911..7318)
                     /locus_tag="PLW13_08825"
     CDS             complement(6911..7318)
                     /locus_tag="PLW13_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY70518.1"
                     /translation="MPTHIPRRRASQDESEIDLTPMLDVVFIMLIFFVVTASFVKETG
                     IHVARPQPQVGEAGKRNILLTVTAGNEIWLGERRVDARSVRATIERLHAGNPEAAVLI
                     RAHEDSDTEVFAGIADQSREAGVYDVSISTFAD"
     gene            complement(7338..8378)
                     /locus_tag="PLW13_08830"
     CDS             complement(7338..8378)
                     /locus_tag="PLW13_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQY70519.1"
                     /translation="MSEEKPLKGIERERVTQWLAEKLPRAAPPFAFRLIAAGGSNLTY
                     RFEDARGGAFILRRPPVAGALATAHDMGREWRIMHALGAHAPQVPVPAMLAFCDDPAV
                     TGAPFYVMEFVDGLILRDRAGAADMDAAACRAAMRSLVATQAAFHMLDPAAVGLGDLG
                     KHEGYVARQLSRWRRQVEASKTRDLPLLGQLHERLSAGIPPETRRAGLAHGDYRFDNT
                     VLGPDHRVRAVLDWELCTIGDPVADFFWSLMYWAEPGDEISFLPDPPTRLPQFPRRAD
                     VVACYEEITGQALPHAAFYTAFGWWKQACIVEGVYARLVKGASGGMKTAPPEQVARIV
                     ERYLERAASALA"
     gene            complement(8388..9701)
                     /locus_tag="PLW13_08835"
     CDS             complement(8388..9701)
                     /locus_tag="PLW13_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70520.1"
                     /translation="MAWDFSTDPDFEADLGWARRFVDETILPLELAGEGLNQRQLDAL
                     WAPFKQQVKERGLWAAHLPPAHGGCGMGQLRLALLHEVLGRSVLAPEIFGCQAPDSGN
                     AELLAAGANAEQARRWLEPLLAGHVRSTFALTEPHNSGSDPTGITTRCEREGEEWVIN
                     GHKWFASNASVSDFMIVMAVTDPEAPPHRRAAMLVVPCRHPGVVLVRDVGSMHHPLAG
                     DALMDRIGGHTEVRFENCRVPLDHMIGAPGDGFVLAQKRLGGGRIHHAMRCIGQADRA
                     LEMMLERAVSRDTRDRPLAKQQLVQEMIAESSAELEMARLLVLRAAWRMDQQGDYSRE
                     ARREIAMVKFVVPRTLLAIIDRAIQVHGALGYTTDMPLEGMYRVARALRIADGADEVH
                     KQTVARELTRGMAAVAEPSEYVPARRRRAGELFGERVRAIRATVS"
     gene            complement(9829..10197)
                     /gene="mutT"
                     /locus_tag="PLW13_08840"
                     /pseudo
     CDS             complement(9829..10197)
                     /gene="mutT"
                     /locus_tag="PLW13_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
     gene            complement(10242..11468)
                     /gene="argJ"
                     /locus_tag="PLW13_08845"
     CDS             complement(10242..11468)
                     /gene="argJ"
                     /locus_tag="PLW13_08845"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HQY70521.1"
                     /translation="MPVGAPSRFPLSAVRGVRLGVARAGVRKPGRRDLVILELAPGSR
                     VAARFTRNAFCAAPVTVAREHLAAAPGGIRYLLVNTGNANAGTGARGMEDARACCREL
                     ARLGAVAEQAVLPFSTGVIGEPLPLERVTGGMPEALAALDAEAWEAAAEGIMTTDTRP
                     KGASRRVRIGTVEVTVSGIAKGAGMIRPDMATMLGFVATDAEVAQAVLEGIVARAVDG
                     SFNCITIDGDTSTNDACVLVATGRAGAPAIASADDPAAALLERAVHEVCLELAQAIIR
                     DGEGATKFVTVAVSGGADHAECRRVAYAVAESPLVKTALFASDPNWGRILAAVGRAGV
                     PGLEVERVGIRLNDVVIVSNGARDPAYTEAAGQRVMAGEEILIAIDLARGAAQATVWT
                     TDLSHDYVRINAEYRT"
     gene            complement(11499..>13703)
                     /gene="secA"
                     /locus_tag="PLW13_08850"
     CDS             complement(11499..>13703)
                     /gene="secA"
                     /locus_tag="PLW13_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQY70522.1"
                     /translation="ITYGTNNEFGFDYLRDNMVFSLDDRMQRELNFAIVDEVDSILID
                     EARTPLIISGAAEDSSRLYKHMNVLVPRMQRGSEEGGDGHFVIDEKLRQVELTEEGHQ
                     FIEQMLISEGLLPEGESLYHAINLGLLHHVHTALRAHHLFQRDVEYIVQNGQVVLIDE
                     HTGRTMAGRRLSEGLHQAIEAKEGVRIQAESHTLASTTFQNYFRLYGKLAGMTGTADT
                     EAFEFRQIYGLDVVVIPTNKPTLRKDLNDLVYLTKEEKFEAVVADIQSCTGKGAPVLV
                     GTASIETSEELSAFLQKQNIAHQVLNAKYHEREAEIIAQAGRPATVTIATNMAGRGTD
                     IVLGGNLEAELAQLADATPEQVAALRVEWQQRHQRVLDAGGLHIIGTERHESRRIDNQ
                     LRGRAGRQGDPGLSRFYLSLEDSLMRIFASDRVKGFMQKLGMERGEAIEHRMVSNAIE
                     KAQRKVEGRNFDIRKQLLEYDDVANDQRRVIYQQRNELLASADVSDTIRQIRADVVNG
                     VISGFIPPQTLEEQWNVSGLEQHLHAEYGLTLSIARWLEEESGLEEQRLRERIVAAVE
                     AGYAERVAPLGADMHKLERHIMLQVLDTLWKEHLSAMDALRQGIHLRAYAARNPKQEY
                     KREAFKLFEELLENVKYEVVRFLSKVQVRSEDEVEALERQQREAQEREAQGARYVHAE
                     APELVPGEEPAGETAQVAAPAPFVRDMPKVGRNEPCPCGSGKKYKQCHGQIA"
BASE COUNT         2127 a         4817 c         4579 g         2180 t
ORIGIN      
        1 cgttgacctt tatgtaggcc agtcccttgg cgccgtagat gccgacgaat cgcgtgtagt
       61 cgtcgatctg cttgcggctg agcagcgagc ctccgcccgg taccttcaac gcggccacgc
      121 gcccgtccgg gtcattggcg ggcgcggaga acaccttgaa gtcgaccgca cgcatcaggt
      181 cactcacctc gaccagctcc agcgggatgc gcaggtcggg cttgtcgctg ccaaagcgcg
      241 ccatcgcctc ggcccaggtc aggcgcggaa attcgccgag gtcgtgcttc agcacctgtg
      301 cgaagagccc gcgcaccatc gcttcggcgg tggcggtgat ctcggcctgg tcggcgaagg
      361 cgaactccag gtcgatctgc gtgaactccg gctggcggtc ggcgcgcaga tcctcgtcgc
      421 gaaagcactt cacgatctgg tagtagcggt ccacgccggc caccatcagc agttgcttga
      481 acagctgcgg cgactgcggc agcgcgaaga agcgccccgg gtgggtacgg ctgggcacca
      541 ggtaatcgcg cgccccctct ggcgtcgcgc gcgtcagcat cggcgtttcg acatcgatga
      601 agccgcgctc ggcgagaaag ttgcgaatcg ccatcgagat gtcggagcgc agccgcaggt
      661 tgttcagcat ctcggggcgg cgcaggtcga ggtagcggta gcgcaggcgc gtctcctcac
      721 cgacattggc gtgctcgtcg agctggaacg gcggcgtgtc ggaaacgttg aggatctcga
      781 gctcgcgcgc gtagacctcg atccgcccgg tcttcatcgc ggggttcacg gtcgcctcgg
      841 cgcgagcgcg cacgcggccg cgtacgcgca ggacgtactc gctgcgcacg cgatcggccg
      901 tggcgaaatg ctcgccgcaa tccggatcga acaccacctg taccacaccc tcgcgatcgc
      961 gcaggtcgac gaagatcacc cctccgtggt cgcggcgccg atctgcccaa ccgcagatcg
     1021 tgacctcctg gtcgacgtga gacgtatcga gatcaccaca gtagtgcgtg cgcatggcgt
     1081 gaatttccgc ggatggggag ggttcgggat gctcaggcgc cgccggcgcc cgacgtgggc
     1141 gcgggcgacg acgcggcggg cttgctgtcg cctccggagg agcccgaatc gccggcgagg
     1201 ttcttcttgc tgccgctctt gaaatcagtc tcgtaccagc cgccaccctt cagccggaaa
     1261 cccgccgcgc tgatcttgcg cttcagttgc ggctcgccgc attccgggca gacggtcaag
     1321 ggttcatccg agatcttctg cagcttttcg agctcgcgat tgcagccctg gcactggtac
     1381 tcgtagatcg gcataccgga aacctcctcg gtctggacat caactacgcg cgcgccccat
     1441 gacgcgaccg cgaaaaagcg gcggattata ctccacccgg tccgcgcgaa ttatgccaac
     1501 tggtgccatt cctggcgcgc caccggaccg gccatggcgc ccctttgcgg ctcgttcatg
     1561 cctcctcagg cgagcgccgc gcgccggcgc aagaaaaatg ccggacgctg ttgccgtgca
     1621 atagtgaatg cattatatat acgggcgctg tagcgcaaag cgcgcgaaaa acctgtaatt
     1681 acagcaatta cacgggcttc tccccccgga agccacactg ccaaacaacc tggaaaccag
     1741 aaggaaccaa catggccgct gacaagaaaa aagccgcgaa ggcagcaccg aagaaggctg
     1801 ctgcgaaggc agctcccaag aaggctgccg cgaaggcagc accgaaggct gctgcaaagc
     1861 cgaccccggt caaggaaagc tggagcaaga cccagatcct caacgagatc gccgagcgca
     1921 cccagctgtc gcgcaagcag gtcaccgcgg tgatggcgga gctcgaaggc atcatgagcc
     1981 gccacatcgc caagaacggc ccgggcgatt tcgtgctgcc tggcctgctc aagatcacca
     2041 cggtaaagaa gcctgccaca aaggctcgca agggcatcga cccgttcacc aagcaggagc
     2101 gcatgtttgc cgccaagccc gcctcggtga aggtgaagat ccgtccgctg aagaagctca
     2161 aggacatggc cggctgaggc tgatccgccg gcctgtctct cgaacccctc tgcgagcaat
     2221 ccaggggggt ttttcgtttc tggagcctcc tgcgccgccg ggtggccacg cgccgacacg
     2281 gtgtaatccg cgccgttgct gcgacattct gcggtctctg taccaccccg ctcgcctgta
     2341 cgctgatccc gcctgaccgt agaatttgcg cctgatccag cggaccccac agcggaaccc
     2401 cagcatgcgc tccagccagc tattgatagc caccctcaag cagacgccgg cggacgccga
     2461 ggtcgtcagc caccagctga tgttgcgcgc cggcctgatc cgccgcctgg catcgggact
     2521 ctatacctgg ctgccgctcg gtctgcgcgt gctgcgcaag gtcgagacca tcgtgcgcga
     2581 ggaaatggac cgcagcggcg cgcaggaggt gctgatgccg gtggtgcagc ccgccgagct
     2641 gtggcaggaa tcggggcgct ggcaacagta cggccccgag ttgctgcgca tcagtgaccg
     2701 ccacgagcgc gcgttctgcc tcggccccac gcacgaggag gtgatcaccg acctcgcgcg
     2761 caacgagttg cgcagctacc gccagctgcc ggtcaacttc taccagatcc agaccaagtt
     2821 ccgcgacgag atccgcccgc gcttcggcgt gatgcgcgct cgcgagttca tcatgaagga
     2881 tgcctattcc ttccacctcg atgccgagtc gctggaccag acctatcgcg ggatgcaccg
     2941 ggcgtactgt cgcatcttcg atcgcatcgg gctgggctac cggcccgtca tcgccgacac
     3001 cggcagcatc gggggcagcg aatcacacga attccacgtg ctggcggatt ccggcgaaga
     3061 cgcgatcgcg ttcagcgacg ccagcgatta cgccgccaac gtcgagaaag ccgaggcgct
     3121 ggcgcccact gcgccgcgcg cgcaccccgc cgcggcgcgc gcgatggtcg ccactcccgg
     3181 caagcacacc atcgccgacg tcgcgacgtt cctcggcgtg cccgaggcgc gcatggtcaa
     3241 gactctggtg gtgaacggct cgagcgaggc ggcgccgctg gtggcgctgg cgctgcgcgg
     3301 cgatcaccag ctgaacgctg tcaaggccga ggcgcacccg ctggtggcct cgccgctgtg
     3361 cctggcctcc gaagccgcga tcacggcggc gcttggctgt cgccccggct cgatcggccc
     3421 ggcggacctc ggcatccccg tcatcgccga ccgcagcgcc gctgcgctcg ccgatttcgt
     3481 gtgcggagcc aaccaggacg ggcaacacct cacgggcgtg aactgggagc gcgactgcaa
     3541 ctacgccgag gtttgcgacc tgcgcaacgt ggtcgacggc gacccgagcc ccgacggcca
     3601 cggcaacatc gtggtgcgtc gcggcatcga ggtcggccac atcttcaagc tcgggcagaa
     3661 gtacagcgcc gcgctaggcg ccagcgtgct ggacgatgcc ggcgcggaac gcaccatgac
     3721 gatgggctgc tatggcatcg gcgtgtcgcg catcgtcgcg gcggcgatcg agcagaacca
     3781 cgacgcggcc ggcatcgtct ggccggatgc catcgcgccg ttcaccgtgg tgctgctgcc
     3841 gctgaacatg cacaagtcgg cgctggtgcg cgagaccgcg gagcggctct atgcgcaact
     3901 cgccgccgcc ggcatcgacg tgctgctcga tgaccgcgac gagcgcccgg gcgtgaagtt
     3961 cgccgacatg gaactgatcg gcatcccgca ccacgtcgtg atcggggagc gctcgctggg
     4021 cgcggggatg ctcgagtaca agcaccgcgc cggcggcgag cgcgaggaag tgccggtggg
     4081 ctcgattggc gatttcctgt ccgcgcgcat caagacggct acggcctgaa ccgggagact
     4141 acgacatgct gaacctgatc gtgctgctcg ccatagcctg gctgctgtgg ctcatccgcg
     4201 aggaactcgc gcagatgagc gaacgccagc gcagcctcaa gctgacgctg gaacgcctgg
     4261 ccgaacgcct cgatacgtcg gggaagaacg agacgtaggt cagccggtgc gcagccagcg
     4321 cgcgacgtcc tcggcgtagt agctgaggat catgtcggcg ccggcgcgcc ggaatgccat
     4381 catcgtttcc agcaccagcg ggcgctcctc catcacgccg gccgcgacgg cggccttgat
     4441 catcgcgtat tcgccactga cctggtagac ggccagcggg cgcgcgcagt gcgcacggat
     4501 gttcgccagc acgtcgaggt aggccagccc cggcttgacc atcaggatgt ccgcgccttc
     4561 ctgctcgtcg cgcaccgatt ccgccagcgc ctcgcggccg ttggccatgt cgagctggta
     4621 gctggagcgg ctgcccttga agctgctgtc caccgcgtcg cggaacgggc cgtagaacgc
     4681 cgaggcgaac ttcgtcgagt aggacatgat cggcagctgc ccgaagccgg ccgaatcgag
     4741 cccgccgcgc agcgcggcga ccatgccgtc catcattccc gagggcgcga tcatgtcgac
     4801 gccggcgtgc gcggcagtca gcgcctggcg caccaggttc tccagcgtcg cgtcgttctg
     4861 cacctggtca ccggcgacca cgccgcagtg cccgtgatcg gtgtattcgc agaagcagtt
     4921 gtcgctgatg accagcatgt ccggcgcggc ctgcttcgcg ctgcggatca tgcgcgccat
     4981 gagcccgtcg ggattccagg tgtcgctgcc gcaggcatcc ttgcgatgcg agacgccgaa
     5041 cagcatgacc gccccgatgc cactgtcgcg cgcgcgcgcc accacctcgc cgagcgcggc
     5101 ttcggggtgg cgcaccacgc ccggcatgct cgcaatgggc accgcggcgg ccacgccctc
     5161 ctcgacgaag accggcagga tcagctgcgc ggaggtgagg gaatgctccc gcaccaggct
     5221 gcgcaggcgc tcggtgcggc gcagccgacg gaagcgaaac ggcatctcgg tcacgcgaag
     5281 gactccggta cggggcaacg acggggcgcg gcaggcgggc tcagacgccc atgctgccga
     5341 gcgcaaccag catgttgttg acgatcgcgg tcaggttggg atggcgggcc tcgaagccgg
     5401 tgatggcctc ttcgagctcg tcgaccaggc cggcgggatc accgaccgca ttctcctcgt
     5461 ccagttcgcg ctggacctgc cccgcaagct cctccaggcg cgcgagcgca tcgcggtcac
     5521 ccgcgtccgc gcgcgcgatt tcctcgcgca ggcgccccag gtcggcgcgc agtctgtcga
     5581 tcgccatatg cactctcccg ctgctgatgg tgccgattat cggccatcgc gcgggcgggg
     5641 catacccctc tgctcagggc gcgccgtcga agaagctcgc gaagccactc ggcgcgtgac
     5701 gcccgaagca gatcgtctcc agagtaccca gccccacgcg ctcgcccatg cgcgcacgca
     5761 ccagttggtg gacatggata ttggggtagt ccagcgggtc gatctcgtcc aagcgccagg
     5821 attcgcccgc gatcgcctcc tcgcccttcc agatcccgtg cccccaggag ggatgcgagt
     5881 aacctatgcc cttcatctgg aacgtgagca cgggctcgag cgtgatccgg tgctcgacgc
     5941 ccgcagcctc gacgagcacg atttccgcgc cttttgcgcg ccgcgtgccg ctggcccagt
     6001 ggatgcggtg ccgcgcggtc tgcatgtgct gcagcgcggg ttcctcggcg ggaatcagct
     6061 ccggcgcacc gtacaccggc accatgcagg cgccgagctg cgtcggatgc ccgtcgcggt
     6121 cctcgaaggt attgaactgc gtggcgacgt caccgaaatc gatcggcgac cacacccagt
     6181 acacgccagg ctcgccgttc aacttgcccg gcgcgccgcc ctcgggctcc ccgaccggcc
     6241 gcacgcccca ggacttgtcg cgcgcgccca gggactgctc cgcacgcacc tcgtggcgca
     6301 caccgccgac cttgaaccag cccgaccagc agccgaactg cgtgaaacga ctgttgtgca
     6361 tgatcagccg gccatcgtcg aacatcaggt tcttcggttc ctcgaccggc acgctgcgcg
     6421 cggcgaacag caggtcgcac tcgatgccgg tttcattcga cacgaggcgc acgcgcacgc
     6481 ggcgcaacgg ctcctgcacc tcgatggcga gcggcccgac cacggtctgg gcacggtccg
     6541 ccggcgcgcg acgcgacccg tggaagctgt gctgcacgcc gtccacgacc acgctgaagt
     6601 gcccatccat cacgcgccgg ttcgggtaca gcccgaagcc gatctcgaac aggaacgcgc
     6661 cgcccgtgtc gaagccgttg aaccagtagc ggtcgtagaa gttgcggtcg ctctgcgagg
     6721 gctgggcgac gggttcgtgg gtctggtgga tgcagtaatc gtcgaaggac gtgatcatcg
     6781 tggtgtctcc cggtcggacg cgctgccgcg cgcgaaggtt gttgcgggca atggccggca
     6841 ctatccatac gcccacccag ggtgacaagt tacgttttgg ccagagtgcg ggcgcgacgc
     6901 cgcggccccg ctagtccgcg aacgtgctga tcgagacgtc gtagacgccg gcctcgcgcg
     6961 actggtccgc tatgcccgcg aagacttcgg tatcggagtc ctcgtgggcg cggatcagca
     7021 ccgcggcctc cggattgccg gcgtgcaggc gctcgatggt ggcgcgcacc gagcgcgcgt
     7081 cgacgcggcg ctcgccgagc catatctcgt tgccggcggt cacggtgagc aggatgttgc
     7141 gcttgcccgc ttccccgacc tgcggctgcg gccgcgcgac gtggatgccg gtctccttca
     7201 cgaacgatgc cgtcacgacg aagaaaatca gcatgatgaa gaccacgtcg agcatcggcg
     7261 tgaggtcgat ctcggactcg tcctggctcg cgcggcggcg cgggatgtgc gtcggcatcg
     7321 gtgttcccgg gcgccgctca ggccagcgcc gacgccgcgc gctctaggta gcgctcgacg
     7381 atgcgcgcga cctgctccgg cggcgcggtc ttcatgccgc cgctggcgcc cttgaccagc
     7441 cgcgcgtaga cgccctccac gatgcaggcc tgcttccacc agccgaacgc ggtatagaac
     7501 gccgcgtgcg gcagcgcctg ccccgtgatc tcctcgtagc aggccacgac atcggcgcga
     7561 cgcgggaact gcggtagccg cgtgggggga tcgggaagga agctgatctc gtccccgggt
     7621 tcggcccagt acatcagcga ccagaagaag tccgccaccg gatcgccgat ggtgcagagc
     7681 tcccagtcga gcaccgcgcg cacgcggtgg tccggcccga gcacggtgtt gtcgaagcgg
     7741 taatcgccgt gcgccagccc ggcacgccgc gtctctggcg ggatcccggc gctcaggcgc
     7801 tcgtgcagct ggcccagcag cggcaggtcg cgcgtcttcg acgcctccac ctgccgccgc
     7861 cagcgtgaga gctgccgcgc gacgtagcct tcgtgcttcc cgagatcgcc gaggccaacg
     7921 gccgcgggat cgagcatgtg gaaggcggcc tgcgtggcca ccagcgagcg catcgcggcg
     7981 cggcaggccg cggcatccat gtctgcagcg ccggcgcggt cgcgcaggat cagcccgtcg
     8041 acgaactcca tcacgtaaaa gggagccccg gtcaccgcgg gatcgtcaca gaaagccagc
     8101 atcgcgggca ccggcacctg cggggcatgc gcgccgagcg cgtgcatgat gcgccactcg
     8161 cggcccatgt cgtgggccgt ggccagtgca ccggccaccg gcggacggcg caggatgaac
     8221 gcgccgccgc gcgcgtcttc gaagcggtac gtgaggttgg agccgccggc ggcgatcagg
     8281 cgaaacgcaa acggcggcgc cgcccgcggc agcttttcag ccagccattg cgtcacgcgc
     8341 tcgcgctcga ttcctttcag cggtttctct tcgctcatgg ctcgtcctca ggacaccgtg
     8401 gcgcggatcg cgcgcacccg ctcgccgaag agctccccgg cgcgccggcg ccgggccggc
     8461 acgtattcgc tgggctcggc gaccgccgcc atgccgcgcg tcagctcgcg cgcgaccgtc
     8521 tgcttgtgca cctcgtcggc gccgtcggcg atgcgcagcg cgcgcgccac gcggtacatc
     8581 ccctcgagcg gcatgtcggt ggtgtagccg agcgcgccat gcacctggat cgcgcgatcg
     8641 atgatcgcga gcagcgtgcg cggcaccacg aacttgacca tcgcgatctc gcggcgtgcc
     8701 tcgcggctgt agtcgccctg ctggtccatg cgccaggccg cgcgcagcac cagaagccgc
     8761 gccatctcga gctccgcgct cgattccgcg atcatctcct gcaccaactg ctgcttcgcg
     8821 agtggccggt cccgggtgtc gcgcgacacg gcgcgctcga gcatcatctc cagcgcgcga
     8881 tcggcctgcc cgatgcagcg catcgcgtgg tggatgcgcc cgccgcccag gcgcttctgc
     8941 gccagcacga aaccgtcacc cggcgcgccg atcatgtggt ccagcggcac gcggcagttc
     9001 tcgaagcgca cctcggtgtg gccgccgatg cgatccatca gcgcgtcgcc cgcgagcggg
     9061 tggtgcatcg agcccacgtc gcgcacgagc accacgccgg gatggcggca cggcacgacg
     9121 agcatcgcgg cacgccggtg cggcggcgcc tcggggtcgg tcaccgccat cacgatcatg
     9181 aagtcgctga cgctggcgtt cgaggcgaac cacttgtgcc cgttgatcac ccactcctcg
     9241 ccctcgcgct cgcagcgcgt ggtgatgccg gtgggatcgg agccgctgtt gtggggctcg
     9301 gtcagcgcga aggtgctgcg cacgtggccg gcgagcagcg gctcgagcca gcgcctggcc
     9361 tgctcggcgt tcgcgccggc ggccagcagt tcggcgttgc cggaatcggg tgcctggcag
     9421 ccgaagatct cgggcgcgag cacgctgcgc ccgagcacct cgtgcagcag cgcgaggcgc
     9481 aactgcccca tgccgcagcc gccatgcgcg ggtggcaggt gcgccgccca caggccgcgc
     9541 tccttcacct gctgcttgaa cggcgcccac agcgcatcga gctggcgctg gttcagcccc
     9601 tcgccggcca gttcgagcgg caaaatggtt tcgtcgacga agcggcgcgc ccagccgaga
     9661 tcggcctcga agtccggatc ggtactgaag tcccatgcca tgcggcagtt cgctctgctg
     9721 gttgtgttcc gggcatgtta cgcgagaccg gggcgcgcgc aattcttgtc acgcgccgcg
     9781 cgccctgata cgcgatcagc gcgcgcgcgc tgaccgatcg gcctgtattg cctcgatgat
     9841 cggcgcattg gccgcgggaa aatcggcggg atcgagtgcc gtgacgggca accagcgcag
     9901 cggctggcct tcgcgcccgt gcgccgtgcc gctgaacgcg agcacatgcc acacctcgag
     9961 gcgcaccgag cgctcgggat aatcgtgttc gatgctgagc agcggcgcag cgtcctcgac
    10021 gcggatgccc agctcttcgt gcagctcgcg cgccagcgct tccagcacgc tctcgccggc
    10081 ggcgaccttg ccgccgggga attcccacag cccgccctgg tgcaggtgct cgtggcggcg
    10141 cgcgaccagc acctcgccgg cggcgttggt gatgacgccc acggccacgc gcagcatggt
    10201 gcggctcccg gcggccccgg gcgacgctgc gctgcgcccg ctcaggtgcg gtattccgcg
    10261 ttgatgcgca cgtagtcgtg cgacagatct gtggtccaca cggtcgcctg tgccgcaccg
    10321 cgcgcgagat cgatcgcgat caggatctcc tccccggcca tgacccgctg cccggcggcc
    10381 tcggtatagg cgggatcgcg cgcgccgttg ctcacgatga cgacgtcgtt caggcgtatg
    10441 cccacgcgct cgacctccag ccccggcacg ccggcacgcc ccacggcggc caggatgcgc
    10501 ccccagttcg gatcgctcgc gaacagcgcg gtcttcacca gcggcgactc ggccaccgcg
    10561 taggcgacgc gacggcactc ggcgtgatcg gcgccgccgc tcaccgcgac ggtcacgaat
    10621 ttcgtcgcgc cctcgccgtc gcggatgatc gcctgcgcca gctccaggca gacctcgtgc
    10681 acggcccgtt cgagcagggc ggcggcaggg tcatcggccg atgcgatggc cggcgcgccc
    10741 gcccggccgg tggccaccag cacgcaggcg tcgttggtcg aggtgtcgcc atcgatcgtg
    10801 atgcagttga agctgccatc gaccgcgcgc gcaacgatcc cctccagcac cgcttgcgcc
    10861 acctcggcat ccgtggcaac gaagccgagc atggtcgcca tgtccgggcg gatcatgccg
    10921 gcgcccttgg cgatgccgct gaccgtgacc tcgaccgtac cgatccgcac gcgacggctc
    10981 gcacccttgg gacgcgtgtc ggtggtcatg atgccttcgg ccgctgcctc ccaggcctcg
    11041 gcatccagcg ccgccagcgc ctcgggcatg ccgccggtga cccgctccag cggcagcggc
    11101 tcgccgatca cgccggtgga gaacggcagc accgcctgtt cggccacggc gccgaggcgg
    11161 gcgagctcgc ggcagcaggc acgcgcatcc tccatgccgc gcgcgccggt gccggcgttg
    11221 gcgttgccgg tgttgacaag caggtagcgg atgccgccgg gagccgccgc caggtgctcg
    11281 cgcgccaccg tcaccggcgc ggcgcagaag gcattgcgcg tgaaacgggc cgcgacgcgg
    11341 ctgcccggcg cgagctccag gatcacgaga tcgcgccggc cgggcttcct gacgccggcg
    11401 cgcgccacgc cgagacgaac gccccgcacg gcggacaggg ggaaacggga aggcgcgccc
    11461 acgggcatcg gttgcggtcc tcgaagtgga tcagccgctc aggcgatctg cccgtggcac
    11521 tgcttgtatt tcttgcccga gccgcagggg cagggttcgt tgcgaccgac cttgggcatg
    11581 tcgcgcacga agggcgcggg cgccgcgacc tgcgcggtct cgcccgccgg ctcctcgccg
    11641 ggcaccagtt cgggcgcctc ggcatgcacg taacgcgcgc cctgcgcctc gcgctcctgc
    11701 gcctcgcgtt gctggcgctc cagggcctcg acctcgtcct cgctgcgcac ctgcaccttg
    11761 gagagaaagc ggaccacctc gtacttgacg ttctccagca gttcctcgaa cagcttgaac
    11821 gcttcgcgct tgtattcctg cttgggattg cgcgcggcat aggcgcgcag gtggatgccc
    11881 tggcgcagcg cgtccatcgc cgagaggtgc tccttccaca gcgtatcgag cacctgcagc
    11941 atgatgtggc gttcgagctt gtgcatgtcg gcccccagcg gtgccacgcg ctcggcatag
    12001 ccggcctcca ccgccgccac gatgcgctcg cgcaggcgct gctcctcgag ccccgactct
    12061 tcctcgagcc agcgcgcgat ggacagcgtc aggccgtact cggcatgaag gtgctgctcg
    12121 agcccgctca cgttccactg ctcttccagg gtctgcggcg ggatgaaacc gctgatcacg
    12181 ccgttgacca catcggcgcg gatctgccgg atggtgtcgc tcacgtccgc cgaggccagc
    12241 agctcgttac gctgctggta gatcacgcgg cgctggtcgt tggcgacgtc gtcgtactcg
    12301 agcagctgct tgcggatgtc gaagttgcgg ccctcgacct tgcgttgcgc cttctcgata
    12361 gcgttgctga ccatgcggtg ctcgatcgcc tcgccgcgct ccatcccgag cttctgcatg
    12421 aagcccttca cccggtccga ggcgaagatg cgcatcaggc tgtcctcgag ggacaggtag
    12481 aagcgcgaca ggcccggatc gccctggcgt ccggcgcgac cgcgcaactg gttgtcgatg
    12541 cgccgcgact cgtgacgctc ggtgccgatg atgtgcaacc cgccggcatc cagcacgcgc
    12601 tggtggcgct gctgccactc aacgcgcagc gccgccacct gttcgggcgt ggcatcggcg
    12661 agctgcgcga gctccgcctc gaggttgccg cccagcacga tgtcggtgcc ccggcccgcc
    12721 atgttggtcg cgatcgtgac ggttgcgggc cgcccggcct gcgcgatgat ctccgcctcg
    12781 cgctcgtggt acttcgcgtt cagcacctgg tgcgcgatgt tctgcttttg caggaaagcg
    12841 gacagttcct cggaggtctc gatcgacgcg gtgcccacca gcaccggcgc gcccttccct
    12901 gtgcacgact ggatgtcggc gaccaccgcc tcgaatttct cttccttggt caggtagacc
    12961 aggtcgttca ggtccttgcg cagcgtgggc ttgttggtcg ggatgacgac cacgtcgagg
    13021 ccatagatct ggcggaactc gaaagcctcg gtatcggcgg tgccggtcat gcctgcgagc
    13081 ttgccgtaga gacggaagta gttctggaat gtcgtcgagg ccagcgtgtg gctctcggcc
    13141 tggatgcgca cgccttcctt ggcctcgatc gcctggtgca ggccctccga caggcgccgc
    13201 ccggccatcg tgcgtcccgt gtgctcgtcg atgagcacca cctggccgtt ctgcacgatg
    13261 tactcgacgt cgcgctggaa caggtgatgg gcgcgcaacg ccgtgtgcac gtggtgcagc
    13321 aggccgagat tgatcgcgtg gtagaggctc tcaccctcgg gcagcaaacc ctcgctgatg
    13381 agcatctgct cgatgaactg gtggccttcc tcggtgagct cgacctggcg cagcttctcg
    13441 tcgatgacga aatgcccgtc gccgccctcc tccgagccgc gctgcatgcg cggcaccagc
    13501 acattcatgt gcttgtacag gcgcgaactg tcctcggcgg cgccggagat gatcaggggc
    13561 gtgcgggcct cgtcgatcag gatcgagtcg acctcgtcca cgatcgcgaa gttcagctcg
    13621 cgctgcatcc ggtcatccag gctgaacacc atgttgtcgc gcaggtagtc gaaaccgaat
    13681 tcgttgttgg tgccgtaggt gat
//
LOCUS       DAORUQ010000084        13703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4360_length_13703_cov_6.619065, whole genome shotgun sequence.
ACCESSION   DAORUQ010000084 DAORUQ010000000
VERSION     DAORUQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_13703_cov_6.619065"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1345)
                     /gene="gabT"
                     /locus_tag="PLW13_08855"
     CDS             complement(68..1345)
                     /gene="gabT"
                     /locus_tag="PLW13_08855"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HQY70523.1"
                     /translation="MSSNTELWQRRAAAVARGVSSATQLFAERAVNAEIWDVEGKRYI
                     DFAGGIGVLNTGHRHPKVLAAIEQQLQRVIHSCFQVMPYEPYIALAEQLNAIAPIEGP
                     AKTLLLSTGAEAVENAVKIARLATGRPGVIAFSGGFHGRTMMAMALTGKVAPYKIGFG
                     PFPADVYHARFPHNWHGVSVADSLADIAHLFKCDIEPSRVAAFIVEPVQGEGGFTVAP
                     PEFMRALRTLCDEHGILLIADEVQSGFARTGRFFAMEHYDVRADLITTAKSIGGGLPL
                     SGVVGRAGVMDAPPPGGLGGTYGGNPVACAAALAVIEVIRSENILARATQMGEQFRAH
                     MQALAAEPQLGIGEIRGLGAMVAFELVRPGTREPDADRTKALTARAAELGLLLLSCGV
                     YYNTVRVLVPITVEDTTFAEGLKLLTQAIRDTA"
     gene            complement(1445..1999)
                     /locus_tag="PLW13_08860"
     CDS             complement(1445..1999)
                     /locus_tag="PLW13_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQY70524.1"
                     /translation="MWKNSTARYGSLQVALHWLMLVLLVAVYLSMELREIFPKGSDSR
                     DLMKSMHFMLGLSVLILVTVRIAARFSGPTPAIVPQPPGWQELLGKLLHLAMYAFMIG
                     MPIAGWLILSAEAKPIPFFGLEVPALVAKNHALAESVEHWHKDIGEWGYYLVGLHAAA
                     ALFHHYVQKDNTLVRMLPWGRKAG"
     gene            complement(2208..3056)
                     /locus_tag="PLW13_08865"
     CDS             complement(2208..3056)
                     /locus_tag="PLW13_08865"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70525.1"
                     /translation="MSPGFVGLGHIGKELALKWALADEEMWVFDVVPDPVQVLVAAGA
                     RAASSMAGLARECDLIGVCVRNDDDVDQLLYGPGGLLENARPGTVVAIHSTVLQDSVM
                     RWNRDAAAKSIRLVDAPVTRNALGTFGYMLAGDDEVIARCRPIMSLGGNTVVEVGAVG
                     AGIALKLCNNLMTYAAIVSAHEALRLAQACGLSAQLMMEIGKVNGVVTPQMSAFLTGR
                     IEAARRTGGSAGTSGGMRMAAAPAADLGKKDLRCALDSAEKLGLRLPATKLHAEIIED
                     VFFGRY"
     gene            complement(3223..4551)
                     /locus_tag="PLW13_08870"
     CDS             complement(3223..4551)
                     /locus_tag="PLW13_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY70526.1"
                     /translation="MTDGAVPATVRSPAYVNYVLCLIFLVAAFNVCDRTIVSVLVDDI
                     KRDLALDDRQMGLVMGFAFSLTYLLAGIPIARLADRYSRPRIIAAALFGWSLMTALSG
                     AAQNFVQLALTRMGVGIGEAGGSPPSHSLLTDYVPPARRARAMSLLSIGALVGMGGGV
                     IYGGWASEQFGWRLALVSVGLPGLVLALVFLFTVDELPRRPADAPGVDEFTGKSLGGV
                     LWQLARTPAFVWLTLGATFVTVVAMGRNFWEPTFLRRVYGMDAAEAGLWYALISPVPA
                     ALGTYLCAWITDRLAQRDRRWYGWFPALCALLLVPLGLAFYLAPADVLAGGVPVGFYF
                     SVAASLVGAGWAPATMAVAQGLVPPSARAVTAASWSMIASFVGTGLGPLLVGDLNMRL
                     EPRYGEEAVRWSLVAVSVLPLLAAASYLLLARSLFPDPSRSKPQCPDPCV"
     gene            complement(4548..4697)
                     /locus_tag="PLW13_08875"
     CDS             complement(4548..4697)
                     /locus_tag="PLW13_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70527.1"
                     /translation="MKTHNAKNTDEDAPGARWSLGRMLIEASCVVMLTLLLTDPVTRV
                     PGGTV"
     gene            4935..5315
                     /locus_tag="PLW13_08880"
     CDS             4935..5315
                     /locus_tag="PLW13_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70528.1"
                     /translation="MRRHLHNCATLLALCAVLLRGVIPLGWMPSADAGALALCTADGL
                     VQLSTADAADLLGQPPAAPDDGHVQPAPCSFAAAGSLAGVPAAVALSQPAPFAGFPAT
                     PYSAPFLPDRPAGNTLARAPPALS"
     gene            5387..7447
                     /locus_tag="PLW13_08885"
     CDS             5387..7447
                     /locus_tag="PLW13_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HQY70529.1"
                     /translation="MTLRPLAAALACAHAFTLASLDAHASRADEGLEEIIVTAVHMRR
                     PLMVTTDPSLPRQPLPAHDGADYLKTIPGFNVIRKGGTDGDPMFRGMAASRVNILLDG
                     ESLLGGCGMRMDPPTAYVFPEAYDRITVIKGPQSVTHGPGNSAATVMFERDSKRLEET
                     GWKANGSVTVASFGREDAVLHVVGGTPQAYAELTGTHAQSDNYEDGSGNEVHSRYERQ
                     SVNAALGWTPDENTSLELSGALSDGEAAYADRMMDGAEFERENVGLKFRKDHLSELVQ
                     RVEAQAYYNYIDHVMDNYSLREFTPSMMMPKPSASNPDRETTGGRLLAELKLGERTRA
                     ELGLDTQANEHAVRSTMNQPMMPFERMDRVDDARFDNLGIFAEVTRALDERSRLIGGL
                     RFDDWEARDERATIRNPMMMMAMPNPTAGETRDDMLESGFLRYEHDMQERPVTVYAGL
                     GHVERFPDYWEIISDKESATSLSAFDTDPEKNTQLDVGLIQRGAKVSTSVSLFYNDIE
                     DYILIESNVRKGMRTATIARNVDATTWGGEAGLSYAIATHWKTDLTLAYTRGENDTDD
                     HALAQQSPFEGRWSLNYDNGTWSAGALVRLVDDQDRYALNQGNVVGQDLGPTDGFGVL
                     SLNGGWKIREGVQLTAGVDNLLDETYAEHISRGGAMLAGFTQTERVNEPGRTAWMKLS
                     LVLD"
     gene            7560..8849
                     /locus_tag="PLW13_08890"
     CDS             7560..8849
                     /locus_tag="PLW13_08890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQY70530.1"
                     /translation="MARIVVMGAGLGGMPAAYDLKHALGKDHEVTLINPSPHFQFTPS
                     NPWVLVGWRTPQQVLVPIGENVQKKGVRFIAQAVTRIDADANRLTLADGSEESYDYLV
                     ICTGPRLAFEEVPGAGPDAHTHSVCTTPHAERAARCYEEFLRNPGPVVVGAVQGASCF
                     GPAYEMAMILDADLRRRKLRDRVPMTFVTSEPYIGHMGLGGVGDSKTLLESELRQRHI
                     RWITNAKVLEAAAEAVKVAEHDDKGALVKEHELPTRFTMLIPAFRGVDAVAAVEGLCN
                     PRGFVVIDEHQRNPRYRNIFAAGVCVAIAPVEATPVPTGAPKTGYMIESMVTAIVHNI
                     RADLAGQPATERATWNAICLADLGDDGVAFVALPQIPPRNMTWAKRGKWVHFAKIAFE
                     KYFMHKMASGNTEPVYEKYVLKALGILRLRSGEHKEL"
     gene            complement(8898..9101)
                     /locus_tag="PLW13_08895"
     CDS             complement(8898..9101)
                     /locus_tag="PLW13_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQY70531.1"
                     /translation="MNLDRFVLGFAGFMVLLSLALGLWVHHYWYFLTAFVGLNLLQSS
                     ITGFCPAAIAARKLGVAPGQAFR"
     gene            9235..10164
                     /locus_tag="PLW13_08900"
     CDS             9235..10164
                     /locus_tag="PLW13_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY70532.1"
                     /translation="MRAFTLIPTLLCTVFSSVAAQAMTLRMPAPGDDVIGEVTMVQIA
                     RHADTLNDYARAYGFGFRELLAANPGINPWVPGEGRKVLMPGEFILPPGPREGVVLNL
                     AEQRLYYYQPDGKTVVTYPVGIGREDWQTPQVSTTVTKVVKDPSWTPPASIRREHAAM
                     GDPLPAVVPPGPDNPLGPWAIRLAAPGYLIHGSNKELGVGMRVSHGCMRLYNEDVEEF
                     AGLIKPGTPVRIIDVPVKVGWKGGAMYVEVHEALEEKRAQHVPEEAVADAISVANRIV
                     GQPTEIDWLEAKAAAVQRSGLPMRVSVQSVASR"
     gene            complement(10239..10622)
                     /locus_tag="PLW13_08905"
     CDS             complement(10239..10622)
                     /locus_tag="PLW13_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70533.1"
                     /translation="MTHFTKVVLFTDSDGRARFREEQVPLPEGTPQARFSALMPSSGL
                     QLRESPVGFRSSFHCTGTPQWVFILRGKMEIGLQDGSSRVFGPGEHFYSADVVPEGVE
                     FDPAVHGHWSRQVGDDELVTVFVRG"
     gene            10724..11473
                     /locus_tag="PLW13_08910"
     CDS             10724..11473
                     /locus_tag="PLW13_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70534.1"
                     /translation="MGNLDGKRIIVTGGAGGIGNAAVRLFAAEGARVACTFNTAAPEV
                     PASVLTARCNIADRAEVNQVFERFAEALGGLDVLVHAAGKHGSQAAELVGEQDWDEMF
                     DLNGKATVWTNQAAFRHMREHGGSIVNMGSVEGVRGFAGNSMYAASRGAVMAWTRSVA
                     LEWGRHGVRVNCVAPAIHTEIFERQRQQMDAATLAMVDAHLAAAIPLGGRMGDPLRDL
                     APVLAFLASDDSRFITGQTLAVDGGLMMLGS"
     gene            11481..12749
                     /locus_tag="PLW13_08915"
     CDS             11481..12749
                     /locus_tag="PLW13_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQY70535.1"
                     /translation="MRRLACWLALAFAFALPAGALDAPRLLDDETAVARVLATVDARL
                     ALMSQVAAAKWLAGNAVSDPAREQAVLEHNATAASALGLATTPAGELLALQMRFAREA
                     QLRLHAKWQRHGCDACEPVPDLATVRQELDALGATQLRAIHLAAPALAAPDFVVRHTA
                     LADERLQIALPAGADRAALLHALAAIRRTAAPSLARVQASGVLRIATTGDYAPFSLER
                     DGRLQGVDIALALALAADLGLEAVFVPTGWPTLMQDLAADRFDVALSGISITPERAAL
                     AFFSLPYHAGGKSIVARCAERARFDTARELDDPAVRVIVNPGGTNEAWVRTNLGRAQI
                     QVHPDNRTIFHEIVAGRADAMVTDDVEVELQARRLPQLCRTLPGTLNRADKAVLMARD
                     AALKGAVDAWLQRQLEAGLPAALLREALAH"
     gene            12881..>13703
                     /locus_tag="PLW13_08920"
     CDS             12881..>13703
                     /locus_tag="PLW13_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HQY70536.1"
                     /translation="MSLFQHYRERYEEAKEEELSLVEYLELCKRDRFAYANAAERMLH
                     AIGTPEYIDTSTDARLSRLFSNRVIARYPAFREFHGMEDTIEQIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSMAERLKKLMERCPIYALKGSPVFESPLGLFDPDEDAEILERD
                     YGIARRYLNVIMSPWALKRLHEYGGDISKFRVVKLRPSVLDQVAVAKTEPGDENNQDI
                     SSLVGKVDIRMLERFSQNDADAYSYSGALCRANQGIMEFVEMFKAPIKVLHPLLTA"
BASE COUNT         2385 a         4848 c         4455 g         2015 t
ORIGIN      
        1 gggtccggat tcatccggac aatcgcaggc ccggattctt tcgggcatcg tccggatgaa
       61 tccggaccta cgcggtgtcc ctgatcgcct gcgtcagcag tttcaggcct tcggcaaagg
      121 tcgtgtcctc gaccgtgatc ggcaccagca cgcgaacggt gttgtagtac acgccgcagc
      181 tcagcagcaa caggcccagt tcggcggcac gcgcggtgag cgccttcgtg cgatcggcgt
      241 ccggctcgcg cgtgccgggc cgcaccagtt cgaaggccac catggcgccc aggcctcgga
      301 tctcgccgat acccagttgc ggctccgctg cgagcgcctg catgtgcgca cggaactgct
      361 cgcccatctg cgtggcgcgt gccaggatgt tctcgctgcg tatcacctcg atcaccgcca
      421 gcgccgcggc gcacgccacc gggttgccgc cgtaggtgcc gccgaggccg ccaggcgggg
      481 gcgcgtccat caccccggcg cgtcccacca cgcccgagag cggcagcccg ccgccgatcg
      541 acttcgcggt cgtgatcagg tcggcgcgca catcgtagtg ctccatcgcg aagaagcggc
      601 cggtgcgagc gaagcccgac tgcacctcgt cggcgatcag caggatgccg tgctcgtcgc
      661 agagcgtgcg cagcgcccgc atgaactccg gcggcgccac cgtgaagccg ccctcgccct
      721 gcaccggctc gacgatgaag gccgccacgc gagagggctc gatgtcgcac ttgaacaggt
      781 gcgcgatgtc cgccagcgaa tccgccacgc tgacgccgtg ccagttatgc ggaaagcgcg
      841 catggtagac gtcggcgggg aacgggccga aaccgatctt gtagggcgcc accttgccgg
      901 tgagcgccat agccatcatg gtgcggccgt gaaagccgcc gctgaaggcg atgacaccgg
      961 ggcgccccgt ggcgaggcgc gcgatcttta ccgcgttctc gaccgcctcg gcgccggtgg
     1021 acaggagcag cgtcttggcg ggcccctcga tcggcgcaat cgcgttcaac tgctccgcga
     1081 gcgcgatgta gggctcgtag ggcatgacct ggaaacagga gtggatcacg cgctgcaatt
     1141 gctgttcgat cgccgccagc accttcgggt ggcggtggcc ggtgttcagc acgccgatgc
     1201 cgccggcaaa gtcgatgtag cgcttgccct cgacgtccca gatctcggca ttcaccgcac
     1261 gctcggcgaa aagctgcgtg gcactgctca caccgcgcgc gacggcggcg gcgcggcgct
     1321 gccagagttc ggtgttgctg ctcatggaat actcccgggc cgcgcgtgcg gcatcatatg
     1381 gacgacgagc ccgggatggt acgaagtgcg ccccggcgcg gcaacaccac gctgggggta
     1441 gccctcagcc cgccttgcgc ccccaaggca acatgcgcac gagggtgttg tccttctgca
     1501 cgtagtgatg gaacagcgcc gcggcagcat gcaggcccac caggtagtaa ccccactcgc
     1561 cgatatcctt gtgccagtgc tccacgctct cggcgagggc gtggttcttg gcgaccagcg
     1621 ccggcacctc caacccgaag aagggtatcg gcttggcttc ggcgctcagg atcagccagc
     1681 ccgcaatcgg catgccgatc atgaaggcat acatcgccag gtgcagcagc ttccccagca
     1741 gttcctgcca ccccggaggc tgcggcacga tcgccggcgt cgggcccgag aagcgcgccg
     1801 cgatgcgcac cgtcaccagg atcagcacgg agaggccgag catgaaatgc attgacttca
     1861 tgaggtcgcg cgaatcgctg cccttcggga aaatctcgcg caattccatc gacagataga
     1921 ccgcgaccag cagcaccagc atcagccagt gcagcgccac ctgcagcgaa ccgtaacggg
     1981 ccgtcgagtt cttccacatg acacggatcc ttcacgctat ggatgatggg ttcattatcc
     2041 tgccggcgcc cgggcgcaac caccgacaaa ggcgcgcact aagtctgcct ccggattaag
     2101 cttcggttaa gcaaggcgtc gcacgccgtg gcgccgggcg acgacggcaa ggccccgcgc
     2161 cacgcgcatt ttgcggacct gcttcgaggc ccagcgccag ccccacgtca ataccgaccg
     2221 aagaaaacat cctcgatgat ctcggcatgc agtttcgtgg ccgggagcct gaggccgagc
     2281 ttttccgcac tgtccagcgc gcacctcagg tccttcttgc ccaggtccgc agcgggagcc
     2341 gccgccatcc tcatcccgcc cgatgttccg gcagagcccc cggtccgtct ggccgcctcg
     2401 atgcggcccg tgaggaaggc gctcatctgc ggcgttacca ctccgttgac cttgccgatc
     2461 tccatcatca gttgcgcgga aagaccacag gcctgggcca gccgcaaggc ttcatgcgca
     2521 gacacgatcg cggcataggt catcaggttg ttgcacagct tcagcgcgat tcccgcgccc
     2581 actgcgccca cctcgacgac cgtgttgcca ccgagcgaca tgattggcct gcagcgcgca
     2641 atgacttcgt cgtctcccgc gagcatataa ccgaaagtac ccaaggcatt gcgcgtgacc
     2701 ggcgcatcga caagtcgaat cgatttcgct gcggcatccc ggttccagcg catcaccgag
     2761 tcctggagca ccgtgctgtg aatcgccacg acggtgcccg gcctggcatt ctccagcagg
     2821 ccgccaggtc catagagcaa ctgatcgaca tcatcgtcat tgcgcacaca gacaccgatg
     2881 aggtcacact cgcgcgccag ccccgccatg ctcgacgcgg ccctggcgcc cgccgccacc
     2941 agcacttgca ccggatccgg cacgacgtcg aatacccaca tctcctcgtc cgcgagcgcc
     3001 cacttcagcg ccagttcctt gccgatatgc cccaatccca cgaatcctgg gctcattttc
     3061 gacacctccg taatgttcat cgagccacac ccgccgcgtt cacgaggact caccggtccc
     3121 ggcgcaggcg ctacggttct ccctcgagcg tgacgcgtca cagcgtgtcg tcggcgagct
     3181 cgcccagcgc caggacaccg gcggtaccgg tgccgcaatg gatcacacgc aggggtcggg
     3241 gcattgcggt ttgctccgtg acggatccgg gaacagcgag cgcgcgagca gcaggtagct
     3301 cgccgccgcc agcaacggca ggacgctcac cgcgaccagc gaccagcgca ccgcttcctc
     3361 gccgtagcgc ggctcgaggc gcatgttgag atcaccgacg agcaacggtc cgaggcccgt
     3421 gccgacgaag cttgcgatca tgctccagct cgccgcggtc acggctcgcg cgctgggcgg
     3481 caccaggccc tgtgccaccg ccatggtggc cggggcccag ccggcgccaa ccagggaggc
     3541 agcgaccgag aaatagaaac cgaccggcac gccgcccgcg agcacgtcgg cgggcgcgag
     3601 gtaaaacgcc aggccgagcg gcacgagcaa cagcgcgcag agcgcgggaa accagccgta
     3661 ccagcgccgg tcgcgctgcg cgagacgatc ggttatccac gcgcagagat acgtgcccag
     3721 cgccgccggc accggcgaga tcagcgcgta ccaaagcccg gcctcggccg catccatgcc
     3781 gtagacgcga cgcaggaagg tcggttccca gaaattgcgc cccatcgcca cgacagtgac
     3841 gaaggtcgcc ccgagcgtga gccagacgaa ggctggcgtg cgcgcgagct gccacagcac
     3901 gccgcccagc gacttgccgg tgaactcgtc cacgcccggc gcgtcggccg ggcgacgcgg
     3961 cagctcgtcc accgtgaaca ggaaaaccag cgccagaacc aggcccggca gcccgacgct
     4021 caccagcgcc aggcgccaac cgaactgttc gctggcccag ccgccataga tcaccccgcc
     4081 gcccatgccc acgagcgcgc cgatgctgag cagcgacatc gcccgcgcgc ggcgcgccgg
     4141 tggcacatag tccgtgagca gcgagtggct cggcgggctg ccgcccgcct cgccgatacc
     4201 gacgcccatg cgcgtcaatg ccaactgcac gaaattctgc gccgcgcccg acagcgcggt
     4261 catcaggctc cagccaaaca gtgccgcggc gatgatgcgc ggacgcgaat agcggtcggc
     4321 gagccgcgca atcgggatac ccgccagcag gtaggtcagg gaaaatgcaa aacccatcac
     4381 cagacccatc tggcgatcgt cgagcgcgag gtcacgcttg atgtcgtcca ccagcaccga
     4441 gacgatggtg cggtcacaga cgttgaacgc cgcgaccagg aagatcaggc agaggacgta
     4501 attgacgtag gcggggctgc gcacggtcgc gggcacggca ccatcggtca tacggtaccg
     4561 cccggcaccc gggtgacggg gtcggtcagc agcagggtca gcatgactac gcacgaggcc
     4621 tcgatcagca tgcgaccgag gctccagcga gcgcccggcg catcctcgtc ggtgttcttc
     4681 gcgttgtgcg ttttcatatt gcgtatccgg ctccccattc ccgcattccc gcattcccgc
     4741 agcttgcgca tcctgccggt gtccttaggg cttgtcgagg cggttgcggt tctccccgga
     4801 tcgggaggcc ttcggcaagg tgaaagactc acccgatcag gttgacgtcc acccgccacg
     4861 ggcggcacta ctgcggtagg caggccggta caacggcgct atagtgccgg ccatccgtga
     4921 tagccgggtc aaccatgcgc cgccacctgc acaattgcgc cacgctgctc gcgctctgcg
     4981 cggtgctgct gcgtggcgtg attccgctgg gatggatgcc ctcggccgat gccggcgcgc
     5041 tggcgctgtg caccgccgat ggcctggtgc agctgtcgac ggcggatgct gcggacctgc
     5101 tcggacaacc gcccgcggcg cccgacgacg gccacgtcca accggctccc tgctcgttcg
     5161 ccgcggccgg ctccctcgcc ggcgtgcccg cggccgttgc gctgtcgcag ccggccccgt
     5221 tcgcaggctt cccggcgacg ccctattccg cgccgttcct tcccgaccgg cccgcgggca
     5281 acacgctcgc gcgcgccccg cccgcgctct cctgaacccc gctcgatgct gatcgccgtg
     5341 gcgcccgtgc gccgcggcac ccgttgcgat tccaggagac acaccgatga cacttcgtcc
     5401 gctcgcggcc gcgctcgcct gcgcccacgc cttcaccctt gcctccctcg acgcccacgc
     5461 cagccgcgcc gacgaggggc tcgaggaaat catcgtcacc gcggtgcaca tgcgcagacc
     5521 tctgatggtc accaccgacc cctcgctgcc acgccagccg ctgcccgcgc acgacggcgc
     5581 cgactacctg aagaccattc ccggcttcaa cgtgatccgc aagggcggca ccgacggcga
     5641 cccgatgttc cgcggcatgg cggcctcgcg cgtcaacatc ctgctcgacg gcgaatcgct
     5701 gctcggcggc tgcggcatgc gcatggaccc gcctaccgcc tacgtattcc ccgaggccta
     5761 cgaccgcatc accgtgatca agggtccgca gagcgtgacg cacggccccg gcaactcggc
     5821 ggccaccgtg atgttcgagc gcgactccaa gcgcctggag gaaaccggct ggaaggccaa
     5881 cgggtcggtg accgtcgcca gcttcgggcg cgaagacgcg gtgctgcacg tggttggcgg
     5941 cacgccgcag gcatatgccg agctgaccgg cacgcacgcg cagtccgaca actacgagga
     6001 cggcagcggc aacgaggtgc actcgcgcta cgagcgccag agcgtcaacg ccgcgctcgg
     6061 ctggacgccg gacgagaaca ccagcctcga actcagcggc gcgctcagcg acggcgaggc
     6121 agcctacgcg gaccgcatga tggacggcgc cgagttcgag cgcgagaacg tcgggctcaa
     6181 gttccgcaag gaccatctct cggaactggt gcagcgggtc gaggcgcagg cctactacaa
     6241 ctacatcgac cacgtgatgg acaactacag cctgcgtgag ttcacgccga gcatgatgat
     6301 gcccaagccc tcggccagca atccggaccg cgagaccacg ggcgggcgcc tgctggccga
     6361 gctgaaactg ggcgagcgca cgcgcgccga gctcggcctc gacacccagg ccaacgagca
     6421 tgcggtgcgc tcgaccatga accagccgat gatgcccttc gagcgcatgg accgcgtcga
     6481 tgacgcgcgc ttcgacaatc tcggcatctt cgccgaagtc acgcgggcgc tcgacgagcg
     6541 ctcgcgactg atcggcgggc ttcgcttcga cgactgggag gccagggacg agcgcgccac
     6601 gatccgcaat cccatgatga tgatggcgat gccgaacccc accgcgggcg agacgcgcga
     6661 cgacatgctg gagagcggtt tcctgcgcta cgagcacgac atgcaggaga ggcccgtcac
     6721 cgtttacgcc ggcctcgggc acgtcgagcg ttttcccgac tactgggaga tcatctcgga
     6781 caaggaaagc gccaccagcc tcagcgcctt cgacaccgat ccggagaaga acacccagct
     6841 cgacgtcggc ctgatccagc gcggcgcgaa ggtctcgaca tcggtgtcgc tgttctacaa
     6901 cgacatcgag gactacatcc tgatcgagtc gaacgtcagg aaaggcatgc gcacggccac
     6961 catcgcgcgc aacgtcgatg ccaccacctg gggcggcgag gccggactga gctatgcgat
     7021 cgccacccac tggaagacgg atctcacgct cgcctacacg cgcggggaga acgacaccga
     7081 tgaccacgcg ctggcacagc agtcgccgtt cgagggacgc tggagcctca actacgacaa
     7141 cggcacctgg tctgcgggcg ccctcgtacg cctggtcgac gaccaggacc gctacgcgct
     7201 gaaccagggc aacgtggtgg gccaggacct ggggccgacc gatggcttcg gcgtgctgtc
     7261 gctcaacggc ggctggaaga tccgcgaggg cgtgcagctg acggctggcg tcgacaacct
     7321 tctcgacgag acctatgccg agcacatcag ccgcggcggc gccatgctcg cgggcttcac
     7381 gcagaccgag cgcgtgaacg agcccgggcg caccgcgtgg atgaagctca gcctcgtgct
     7441 cgattgacct ggatcagcat ccgcccgcca tccgccccct gttgttcggg gcggatgccg
     7501 gcgtaccatc ggcactccaa cgcatcgacg cggccatggg ccgcgcaacg gagtcccgca
     7561 tggctcgcat cgtggttatg ggcgccggcc tcggcggcat gccggccgca tacgatctca
     7621 agcacgcgct cggcaaggac cacgaggtca cgctgatcaa tcccagcccg cacttccagt
     7681 tcacgccctc gaacccgtgg gtgctggtgg ggtggcgcac gccgcagcag gtgctggtgc
     7741 cgatcggaga gaacgtgcag aagaaaggcg tgcgcttcat cgcgcaggcc gtgacgcgca
     7801 tcgacgccga cgccaaccgc ctgacgctgg ccgacggcag cgaggagtcc tacgactacc
     7861 tggtcatctg caccggcccg cgactggctt tcgaggaagt gcccggcgcc ggtcccgacg
     7921 cccacacaca cagtgtctgc accacgccgc acgccgagcg cgccgcgcgc tgctacgagg
     7981 agttcctgcg caatcccggt cccgtagtgg tcggcgccgt gcagggggcg agctgcttcg
     8041 gcccggccta cgagatggcg atgatcctcg acgcggatct gcgcaggcgc aagctgcgcg
     8101 accgcgtgcc gatgaccttc gtcaccagcg agccctacat cggccacatg ggactcggcg
     8161 gcgtcgggga ctccaagacc ctgctggaat cggagttgcg ccagcgccac atccgctgga
     8221 tcaccaacgc gaaagtgctg gaagccgccg ccgaggcggt gaaggtcgcc gagcacgacg
     8281 acaagggcgc cctggtgaag gagcacgaac tgccgacgcg cttcacgatg ctgatccccg
     8341 cgttcagggg cgtggatgcg gtggcggccg tcgaagggct gtgcaacccg cgcggcttcg
     8401 tggtgatcga cgagcaccag cgcaacccca ggtaccgcaa catcttcgcc gccggcgtct
     8461 gcgtcgcgat cgcgccggtc gaggcgacgc cggtgcccac gggcgcgccc aagaccggct
     8521 acatgatcga gtcgatggtg accgcgatcg tgcacaacat ccgcgccgac ctcgcgggcc
     8581 agccggccac ggagcgcgcg acctggaacg cgatctgcct cgccgacctc ggcgacgacg
     8641 gcgtggcctt cgtggcgttg ccgcagatcc cgccgcgcaa catgacctgg gccaagcgcg
     8701 gcaagtgggt gcatttcgcc aagatcgcct tcgagaagta cttcatgcac aagatggcga
     8761 gcggcaacac cgagcccgtc tacgagaaat acgtgttgaa ggcgctggga atcctgcgcc
     8821 tgagatcagg cgagcacaag gaactgtaag gacgcggcgc gtgcccggcg ccgtcgggca
     8881 cgcgcctcgt cctcccctca gcggaatgcc tgtcccggcg ccacgccgag cttgcgtgcc
     8941 gcgatcgccg ccgggcagaa gccggtgatg gaggactgca acaggttcag cccgacgaag
     9001 gccgtgagga agtaccagta atggtgcacc cacaggccga gcgcaaggct cagcagcacc
     9061 atgaaaccgg caaagccgag cacgaagcgg tcgaggttca tcgcataccc tcctggcggg
     9121 tcagtacgcg gccaaatatc aggctaatct aatgtgtttt caatcctgat gggggacgtt
     9181 tcaggcattg cctgctagaa tctgccccag tcccccaact ccagatgctt ccagatgcgc
     9241 gcattcaccc tgatcccgac cctgttgtgc accgtttttt cgtccgttgc cgcccaggcc
     9301 atgaccctgc gcatgcctgc ccccggcgac gacgtcatcg gcgaggtcac gatggtgcag
     9361 atcgcccgcc acgccgatac cctcaacgac tatgcccgag cctacggctt cggctttcgc
     9421 gagttgttgg cggcgaaccc cggcatcaac ccgtgggtac cgggcgaagg gcgcaaggtg
     9481 ctgatgcccg gcgagttcat cctgccgccc ggaccgcgcg agggcgtggt cctcaacctg
     9541 gccgagcagc gcctgtacta ttaccagccc gacggcaaga ccgtggtcac gtaccccgtg
     9601 ggcatcggtc gcgaggactg gcagacgccc caggtgagca cgacggtgac caaggtggtc
     9661 aaggacccga gctggactcc cccggcctcg atccggcgcg agcacgccgc gatgggcgat
     9721 ccgctgcccg ccgtcgtgcc ccccggaccg gacaatccgc tgggcccgtg ggcgatccgg
     9781 ctcgcagcgc cgggttacct gatccacggc agcaacaagg agctcggcgt cggcatgcgc
     9841 gtgagccacg gctgcatgcg cctttacaac gaagacgtcg aggaatttgc cgggctcatc
     9901 aagcccggga ccccggtgcg catcatcgac gtgccggtga aggtgggctg gaaaggcggc
     9961 gcgatgtatg tcgaggtgca cgaggcactc gaggaaaagc gcgcgcaaca cgtgcccgaa
    10021 gaggccgtgg ccgatgcgat ttccgttgcg aaccggatag tcggccaacc caccgagatc
    10081 gactggctcg aagccaaggc tgccgccgtg cagcgttcgg gcttgccgat gcgcgtttcg
    10141 gtccagagcg tcgcctcgcg ctgaccctgc tcaccggggc ggagcccggg acgcctgcgc
    10201 acattccggc gggcagcagg ctccagcccc ggcggtgctc agccgcgcac gaacaccgtc
    10261 accagctcat cgtcgccgac ctggcggctc cagtgtccgt gcaccgccgg atcgaactcc
    10321 acgccctcgg gcacgacgtc ggctgaatag aaatgctcgc cggggccgaa cacccgcgag
    10381 ctgccgtcct gcagcccgat ctccatcttg ccgcgcagta tgaacaccca ctgcggcgta
    10441 ccggtgcaat gaaagctgct gcgaaaaccc acggggctct cgcgcaattg caggccactg
    10501 ctcggcatca gcgccgagaa gcgcgcctgc ggcgtgcctt cgggcagcgg cacctgctcc
    10561 tcgcgaaagc gggcgcgccc gtcgctgtcg gtgaacagta cgaccttggt gaaatgcgtc
    10621 atcggggaat ctcgctgccg ttgcgggtga cggcattcta gccccgcccc gtggcgggcg
    10681 tgtataaagc cgactccatc caaatcgacc agggaggaaa cccgtgggca atctcgacgg
    10741 caagcgcatc atcgtgaccg gcggcgccgg cggcatcggc aacgcggcgg tgcggctgtt
    10801 cgccgccgag ggggctcgcg tggcctgcac cttcaacacc gccgcgcccg aggttcccgc
    10861 gagcgtgctc acggcgcgct gcaacatcgc cgacagggcc gaggtgaacc aggtattcga
    10921 gcgcttcgcc gaagcgctcg gcgggctcga cgtgctggtg catgccgccg gcaagcacgg
    10981 cagccaggcg gcggaactgg tcggcgagca ggactgggac gagatgttcg acctcaacgg
    11041 caaggccacg gtgtggacca accaggccgc gttccgccat atgcgcgaac acggcggctc
    11101 gatcgtgaac atgggttccg tcgaaggtgt gcgcggcttc gccggcaact ccatgtacgc
    11161 ggcctcgcgc ggcgccgtga tggcctggac gcgctcggtc gcgctggaat ggggccgcca
    11221 cggtgtgcgc gtcaactgcg tagcgccggc gatccacacc gagatcttcg agcgccagcg
    11281 ccagcagatg gacgccgcca cgctcgcgat ggtcgacgcg cacctcgccg ccgcgattcc
    11341 gctggggggc cggatgggcg atccgctgcg cgacctggct ccggtgctcg cctttctcgc
    11401 ctctgacgac tcgcgtttca tcaccggcca gaccctcgcg gtcgacggcg ggctgatgat
    11461 gctgggctcc tgagcagcgc gtgcgccggc tcgcctgctg gctggccctc gccttcgcgt
    11521 tcgccctgcc cgcaggcgcg ctcgatgcgc cgcggctgct ggacgacgag accgcggtag
    11581 cgcgcgtgct cgccaccgtc gatgcgcgcc tggcgctgat gtcgcaggtc gcggcggcaa
    11641 aatggctcgc gggcaacgcg gtgagcgatc ccgcgcgcga acaggcggtg ctggagcaca
    11701 acgccacggc ggccagcgcg ctgggactcg ccacgacgcc ggcgggcgaa ctgctggcgc
    11761 tgcagatgcg ttttgcgcgc gaggcgcagc tgcggctgca cgcgaagtgg cagcgccacg
    11821 gctgcgacgc ctgcgagccg gtacccgatc tcgccaccgt gcgacaagag ctggatgcgc
    11881 tgggcgccac gcagctgcgc gccatccatc tcgccgcgcc ggcgctggca gcgccggact
    11941 tcgtcgtacg ccacacggcg ctggctgacg agcggctcca gatcgcgctg ccggccggcg
    12001 ccgatcgcgc ggcgctgctg cacgcgctgg ccgcgatccg ccgcaccgcc gcgccgtcgc
    12061 tcgcgcgcgt ccaggccagc ggcgtgctgc gcatagctac caccggcgat tacgcgccgt
    12121 tcagcctgga acgcgacggc cgcctgcagg gcgtcgacat cgcgctggct ctggcgctgg
    12181 cagcggacct gggactggag gccgtgttcg tgcccaccgg ctggcccacc ctgatgcagg
    12241 atctcgccgc cgaccgcttc gacgtggcgc tgtccggcat ctcgatcacg cccgagcgcg
    12301 ccgccttggc gttcttctcg ctcccgtacc acgccggtgg caagagcatc gttgcgcgct
    12361 gcgccgagcg cgcacgcttc gataccgcgc gggagctcga cgatcccgcc gttcgcgtga
    12421 tcgtgaatcc cggcggcacc aacgaggcat gggtgcgcac caacctgggg cgggcgcaga
    12481 tccaggtgca ccccgacaac cgtacgatat tccacgagat cgttgccggt cgcgccgacg
    12541 cgatggtcac cgacgatgtg gaagtcgaac tgcaggcacg ccgcctcccg caactgtgcc
    12601 gcacgctgcc cggcacgctg aaccgtgccg acaaggccgt gctgatggca cgcgacgccg
    12661 cgctgaaagg cgccgtcgat gcctggctgc agcgccagct ggaggccggc ctgcccgcag
    12721 cgctgctgcg cgaggccttg gcgcactgat tgcatgagct cctgcagacg agcgaagcag
    12781 gcccaccgcg cgtccatttg cacacatcgc gcagaggcgc ggaatcatta cgggtggtcc
    12841 atactcaaac caagcatcat cacttcgtcc ggaggtatcg atgagcctgt tccagcacta
    12901 ccgcgaacgc tacgaagaag cgaaggaaga ggaactgtcg ctggtggagt acctcgaact
    12961 gtgcaagcgc gaccgcttcg cctacgccaa tgccgccgaa cgcatgctgc acgcgatcgg
    13021 cacgccggag tacatcgaca cctccaccga cgcgcgcctc tcacgactgt tctcgaaccg
    13081 cgtgatcgcg cgctacccgg cgtttcgcga gttccacggc atggaggaca ccatcgagca
    13141 gatcgtgtcc tacttccgcc acgcggcgca ggggctcgag gagaagaagc agatcctcta
    13201 cctgctcggc cccgtcggcg gcggcaagtc atcgatggcc gaacggctga agaagctcat
    13261 ggaacgctgc cccatctacg cgctcaaggg ctctccggtg ttcgagtcgc cgctcgggct
    13321 gttcgacccg gacgaggacg ccgagatcct cgagcgtgat tacggcatcg cgcggcgcta
    13381 cctcaacgtg atcatgtcgc cgtgggcgct gaagcggctg cacgagtacg gtggcgacat
    13441 cagcaagttc cgcgtcgtga agctgcgccc ctcggtgctc gaccaggtcg cggtggcaaa
    13501 gaccgagccc ggcgacgaga acaaccagga catctcctcg ctggtgggca aggtcgacat
    13561 ccgcatgctg gagagatttt cccagaacga cgccgatgcc tacagctatt cgggcgcgct
    13621 gtgccgcgcc aaccagggca tcatggaatt cgtcgagatg ttcaaggcgc cgatcaaggt
    13681 actgcatccg ctgctgacgg cga
//
LOCUS       DAORUQ010000085        13682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4372_length_13682_cov_7.170984, whole genome shotgun sequence.
ACCESSION   DAORUQ010000085 DAORUQ010000000
VERSION     DAORUQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4372_length_13682_cov_7.170984"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..878)
                     /locus_tag="PLW13_08925"
     CDS             complement(60..878)
                     /locus_tag="PLW13_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HQY70537.1"
                     /translation="MEHLPASFGGLAAVAFLLGLKHGFDADHLATIDGLTRVNTRAGR
                     AFARYCGALFSIGHGAVVVAIALLVSSAVARWQTPAWLETTGVTISIFFLTLLGVLNL
                     RAVWRAAPDEIVSPVGFKGRYLGALGRARNPFVVAFVGALFAISFDTISQAAFFALAA
                     GQFGGVLYAASLGLLFVLGMLVTDGANGLWISRLIVRADETARIASRVMSLAVGSVSL
                     LVAAFSAAKLLLPRVAGWADGKELAFGALVVAIVLAGFLWGRAAARGRALPYAS"
     gene            complement(929..1387)
                     /gene="nikR"
                     /locus_tag="PLW13_08930"
     CDS             complement(929..1387)
                     /gene="nikR"
                     /locus_tag="PLW13_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HQY70538.1"
                     /translation="MQRVTISLDDDLAVAFDKLIARRGYGNRSEAVRDLIRQRLDREA
                     QDRAPRGHCVASLSYVYNHHARDLAERLAERQHAHHDLVVATLHVHLDHENCLESAVL
                     QGPIAAVRALAAGLTAEPGVRHGNLNLVAVEHVDHHAHGGLSHRHLKPKY"
     gene            1418..2332
                     /locus_tag="PLW13_08935"
     CDS             1418..2332
                     /locus_tag="PLW13_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70539.1"
                     /translation="MRVAALCAIALLLAIVVAPPLLEATVLLAALHDAAHVLTFALIA
                     ALLCVLMEPQRAGLVALLVVVALLVVVAVLAVGTELAQPYFAGATSIEVASMGDVGRD
                     LLGGVIGVLTWFAFHRRRHGLWIPAALLLAAGLAPLAFTGWAYAQRALHPEIIWEARR
                     ATWQVFLEPGADGTYSRMQSVPRLRFTATGDSYAGLSIREPPPDWRGYEALQVALANP
                     GAAPIRINVRIDDQPRNTEYEDRFNRERTLPAGASVVWRIPMDEIGQGPKGRRLDLSD
                     IRRVVIFLSPGSRGASFDLGSVRLERAP"
     gene            2347..3126
                     /locus_tag="PLW13_08940"
     CDS             2347..3126
                     /locus_tag="PLW13_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQY70540.1"
                     /translation="MDQLLALLSQPAAWAALATLVAMEVVLGIDNLIFISILTNKLPE
                     QHRSRARRIGIALALILRLALLGTIAIIVRLTTPLFEALGHGFSWRDLILIAGGLFLV
                     WKATTEIHQSVDPDAASHVLVGSEATIGFSSAIMQILLLDLVFSLDSIITAVGMTEHI
                     PIMVIAVIVAVLAMLLAADPLAKFIHDNPTIVMLALGFLLMIGMTLIADGFGAHVPRG
                     YVYAAMAFSAFIEGLNMFARRAKRRRRQRRLAGQAQTQSSS"
     gene            complement(3099..3773)
                     /locus_tag="PLW13_08945"
     CDS             complement(3099..3773)
                     /locus_tag="PLW13_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HQY70541.1"
                     /translation="MSSKSPPVPAPAHPTPDSPASERNKRPILEVIGPAFAQSRRVLE
                     IGSGTGQHAVFLARALSHLLWQPSDRAAWLPALRARIAREGPGNLTTPIELDVSMPRW
                     PVVTADAVYSANTLHIMAWREVEAFFAGVGRLLASSPMEAAAGEPAAACARRMLAVYG
                     PFKYRGAFTTDSNAAFDESLKARDPASGIRDFEAVDALARAEGLVLAADHAMPANNQL
                     LLWVCA"
     gene            3767..4336
                     /locus_tag="PLW13_08950"
     CDS             3767..4336
                     /locus_tag="PLW13_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQY70542.1"
                     /translation="MTWLVFGLILFLGVHSVSIIAPAGRDRMAAALGNGWRALYSIAS
                     IVGFGLLIWGYGVARQEPVLLYQPAPWMRHLTSLLMLPVLPLLFAAYLPGRIKAKLQH
                     PLLVAVKLWAVAHLLANGTLADVVLFGSLLAWAVLDRISFKSRVQRPIPSAPASRWND
                     LVAIVLGLAAYVVFAMVLHVRWIGVPVVP"
     gene            4348..6030
                     /locus_tag="PLW13_08955"
     CDS             4348..6030
                     /locus_tag="PLW13_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY70543.1"
                     /translation="MNTFRALGLPEPLLAALADVGYESPSPIQAATIPVLLAGSDLVG
                     QAQTGTGKTAAFALPLLARLDLAQRAPQVLVLTPTRELAIQVAEAFQRYAAHLPGFHV
                     LPVYGGQGYGPQLHALKRGTQVIVGTPGRVMDHMKRGTLVLGELRALVLDEADEMLRM
                     GFIEDVEWILGETPEARQVALFSATMPAPIRRIAQKYLRSPAEVTIGAKTTTAANIRQ
                     RYWMVSGLHKLDALTRILEVEPFEGMLVFARTKQATVELAERLEARGFAAAALNGDVP
                     QMQRERIVAQLKSGEIDIVVATDVAARGLDVERIGHVVNFDVPTDPESYVHRIGRTGR
                     AGRSGEAILFIAPRERNLLRAIERATRQTIEPMSLPTVEDVNVKRVARFRERIGAALE
                     AGAGAPYRALIEMFARELEVPEVEIAAALAAMMQGKTPLLLQPEARERREQQAPREHD
                     PRPAREHPARERPAREGTARRSARLADVEMETFRVEVGRAHGVRPANIVGAIASEAGL
                     AGKHIGRVDIRGEHSFVDLPGGMPKEILRALQKVIVMGQPLRISRMKRKPRA"
     gene            complement(6044..9301)
                     /locus_tag="PLW13_08960"
     CDS             complement(6044..9301)
                     /locus_tag="PLW13_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQY70544.1"
                     /translation="MARAFALLLLAAIAPCAPAAAIELPPACAKQASFEGTAMPLTEL
                     RQQVEKVGESDPMTAVRILCSTLPRAEREYGADSLEFAWWVQTLATPLIAYMDEFAEA
                     IPLLRFVTPIYERRLGPNAPEIAEIHVAYAWIAFRQGRLADAGDEWERALHIRESVPG
                     ARQIELQKALVGLAQVRVGQRRFADARTLLERAQAILAANGETSSEAAGAIENTLTNI
                     AMREEDYPAARRHAEVQLKIELALRAQRGPAQPVTAYTLYGQVLEKLDEYAEAESALR
                     EAVRLAEATDGPLQRHYRIALTHLGALLGARGKPQEALPVARRALDVAVEQLGPDAPN
                     LVNVLLDLAEVERTLGEMPQAFAHYGRAGQLIAAHGADVERQVQVRHYRGLGLLQYQL
                     GAYDDARASLAAGLKVAGDDPALSVERAGVLLALAHLDATTEPERARAQIGQALALYA
                     ARLPAAHPVLLRVVNDLCGIDIRTAPATAPNCLDAQRRLEAAREVEPGLRYAVYANAS
                     ALAEARGNGGAAWLHAVRALSAAATLGTPDPAWQAQYRLATLLHHRGDDSLAIFFGKQ
                     AIDSIEKLRAWFGEDAQQLQAGFLRDKIGVYRATADWLMQTGRMDEGLQVLDLLKAQE
                     LYDFVLRGDTKAEADEDRVALTQDERQLLTRYTRLLRADAQAGATIDTLAHRRDAGRI
                     TPQESRHLGQLLQGQDAVEAARAKRISEFVASETGGSIGTTQRNITTKRLARELQRFG
                     PESAIGVYLLTERHLRILVATRTEQTEHLIEVDAPALRREIGDLLDAISRREDIRVRS
                     RKLYDLLARPLVEAAHKASAKRLVLWPDDALRYVPFAALHDGRKYLVEDFAIQMYADA
                     AGQPGQADGTKPDLEVLGLGVTRSVGGFQALPAVADELCDIVRGPITGLEIPGKACPA
                     PAIGDGALPGKGYANAAFTEARLGQLLEGPHDYSILHLGTHFSLRPGNALRSFLLLGD
                     GGRLTLDKISALDFAGIALATLSGCQTAMGGAVAADGREIEGLSAIVQRRGASSVIAS
                     LWRVEDQSTASLMRRMYDEMAAGTDVARALQLAQLSLADRHPYYWAGFLLTSSSQ"
     gene            complement(9301..9945)
                     /locus_tag="PLW13_08965"
     CDS             complement(9301..9945)
                     /locus_tag="PLW13_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70545.1"
                     /translation="MSDQADDQAWLDALAGRDALAGRETAGVRAEGARAATLNEARAL
                     RAALHARTRADELSLQREIGRDEETRAALLLERARRDEILAPALKRTSGTAPRKLTGR
                     WGALLAAGIAGLAVALVWTLRPAVEAPIMRGAPDAIHRMVADDPAALRSQIVSALRDA
                     GVEPASYERFGRAGLDADLPRPLTPAVRTVLERFGVPPPGDSVLRIEIAPRPGS"
     gene            complement(9942..10532)
                     /locus_tag="PLW13_08970"
     CDS             complement(9942..10532)
                     /locus_tag="PLW13_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY70546.1"
                     /translation="MRACRDGGSALEQAMRTLDREHFAVLYRESRRTVRDAAAARDLV
                     QETFIKVWQRCASFHGSSELLPWLRAILRHTMLDSFRRHDRAVSLDDAVLTAEVEQKI
                     LELSARAVSTPLDEVQVRQQREVFQRCWAAFERDCPTHAAVIAWIGEDGLDNEAIAQL
                     LGRTPGATREFISQCRKKARVYLAEWYQLAFGAQAS"
     gene            10720..10971
                     /locus_tag="PLW13_08975"
     CDS             10720..10971
                     /locus_tag="PLW13_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70547.1"
                     /translation="MLALLAACGGLTACDQGRWIELPAMLMTCNDLDDKQMTAELANP
                     DKFSLGRGMLQGVCTQSGAGQFTGETRCQKEAVEIRCKS"
     gene            10968..11372
                     /locus_tag="PLW13_08980"
     CDS             10968..11372
                     /locus_tag="PLW13_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70548.1"
                     /translation="MSARHCASAGYLMAALAALSIAGCGGSPSGSGNKDAATLPTSRG
                     DVWTIDPASPPDAQVLAFLHGVHNFVLKGDEIYTATTRLDPAPAAGAVAAATLAGGLA
                     LHIEAPGGQPILVFSSGQRAPLRKFQAPEGSR"
     gene            11369..12577
                     /locus_tag="PLW13_08985"
     CDS             11369..12577
                     /locus_tag="PLW13_08985"
                     /inference="COORDINATES: protein motif:HMM:NF027470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HQY70549.1"
                     /translation="MKAHLTGFALSFTLLIGLTCAGGAQAAGRDFDPEQLALTYHKLA
                     GKPFDFDRYAASSNVVARASGFDRPDVLKQEVARVRAVFDAGSDSIIFETDIQNSISD
                     YDHDKGEFSIAIFEPGSYMPVSFYGQQYRVVFANAEAARAIRMPKEEAREFDRGLMRD
                     GSRGVVSSVKFRVVGDGDPAGAVDGQNVVRAELVSTKTTDTDGTVLSTPDLAAAPAGA
                     APVAAFDARTIDAAGMRIGVSAEDLQAAITRLYGKPDRTPRGPRSDYDPRFAGEIELD
                     LMRCIYVQGQRDHEPVPGDICLRAYYDKDGIVRQVRIQRVLPRFDYEQARAAAIARFG
                     PAAALRGGNTPELSWGSKDPKLGPALVLRIDPYGNTLSFGYSEVRAGSLDLTLTDVAW
                     AAATAALSAN"
     gene            12589..>13682
                     /locus_tag="PLW13_08990"
     CDS             12589..>13682
                     /locus_tag="PLW13_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70550.1"
                     /translation="MKLQILAASLIAGLAMTLTTPSFAAAGGACAGRAHCEETRSFVA
                     DMSNFRVSKSGRYRVLTATISFRNKTGKPLVLGYVNDSGVALDERGNRYVVANPQAVR
                     GMGVVTGSSFDPKFVLAPGEAADTRFELIWDPGREIVGVTYELDLAVREINEVAGNQY
                     KLGVEYAVHFSQLAEGGTASAVATTAAPAAAAPAAAVSAGGATDPCGGSPRCYNAGAF
                     TAEIAQLSATAMGSGVRHHSISINIRFRNVSNAPIVLGYKSGSSAAIDNFGNRYYWGR
                     AGTHDTSVKGIGFVSGRTADTQFQLQPGQSRNATFNLIRFEARPPIGSAWGYDVVIDE
                     LEILPGQQVRTARENSLNFQSLTARAFASAA"
BASE COUNT         2156 a         4544 c         4699 g         2283 t
ORIGIN      
        1 gcgtcgtggc gccgcctgcc gtcgatcccg gttgtgaccc tgcagtccct gcggcgcgat
       61 caggacgcgt agggcagtgc gcgcccgcgc gccgccgcgc gaccccacag gaagcctgcg
      121 agcacgattg cgacgacgag cgcgccgaag gcgagctctt tgccgtcggc ccacccggcg
      181 acgcgcggca gcagcagctt cgccgcgctg aaggccgcga ccagcaggct cacggagccc
      241 accgcgaggc tcatgacgcg tgaggcgata cgcgcagttt cgtccgcgcg cacgatgagg
      301 cgggagatcc agagtccgtt cgcgccgtcg gtcacgagca tgccgagcac aaagaggagg
      361 cccagcgacg ctgcgtacag cacgccaccg aactgccccg ccgcgagcgc gaagaacgcg
      421 gcctggctga tcgtgtcgaa ggagatggcg aagagggcgc cgacgaaggc gacgacgaac
      481 ggattgcgcg cgcgtcccag tgcgccgagg tagcggccct tgaagccgac cggggagacg
      541 atctcgtcgg gcgcggcccg ccagacggca cgcaggttca gcacgccgag cagggtgagg
      601 aagaagatcg agatcgtgac acccgtggtc tcgagccagg ccggtgtctg ccagcgcgcg
      661 accgccgaac tcacgagaag agcgatcgca acgacgacag cgccgtgacc gatcgagaag
      721 agcgccccgc agtagcgcgc gaaggcgcgg cccgcgcgcg tgttgacgcg cgtaagcccg
      781 tcgatggtgg cgagatggtc ggcgtcgaag ccgtgcttca ggccgagcag gaaagcgacg
      841 gccgcgagac cgccgaaaga agcaggcagg tgttccatgg cggcccgagt atgacgattc
      901 acaaaatcgt catacaaggc cgctgcggtc aatacttagg cttcagatgg cggtggctga
      961 gaccgccgtg cgcgtggtgg tcgacgtgct cgacggctac gaggttgaga ttcccgtgcc
     1021 gaacgcccgg ctccgccgta aggcccgcgg cgagcgcgcg tacggccgcg atcgggccct
     1081 gcaggacggc gctctcgaga cagttctcgt gatcgaggtg cacgtgcagg gttgcgacca
     1141 cgagatcgtg gtgggcgtgc tggcgctcgg ccagtcgctc ggcgaggtcg cgcgcgtggt
     1201 ggttgtagac ataggagagg cttgccacgc agtggccacg cggagcgcga tcctgcgcct
     1261 cgcgatcgag acgctggcgt atgagatcgc gcacggcctc actgcggttc ccatagccac
     1321 gccgcgcgat cagcttgtcg aaagccacag cgagatcatc gtcgagggaa atggtgacgc
     1381 gttgcatggg ccgagtttat tgcatactgg cgcccccatg cgcgtcgccg ccctctgcgc
     1441 catcgccctg ctgctcgcca tcgtggtggc gccgccgtta ctcgaggcga cggtgctgct
     1501 cgccgcgctc cacgatgccg cacacgtact cacattcgcg ctgatcgcgg cgctcctttg
     1561 cgtcctcatg gaaccgcagc gtgcagggct tgtggcgctc ctcgtcgtag tcgcgctcct
     1621 cgtcgtggtt gcggtcctcg ccgtcggcac ggagctcgcg cagccttatt tcgcgggcgc
     1681 gacgtccatc gaagtcgcct cgatgggcga tgtcggccgg gacctgctcg ggggggtgat
     1741 cggcgtactc acctggttcg ctttccaccg ccggcggcac ggattgtgga ttcccgcagc
     1801 gctgctgctc gcggcagggt tggcgccgct cgccttcacc ggctgggcct acgcgcagcg
     1861 cgcgctccat ccggagataa tctgggaggc ccggcgtgcg acctggcagg tgttcctcga
     1921 gccaggggcc gatggaacgt attcgcgtat gcagagcgtg ccgcgccttc gcttcaccgc
     1981 caccggcgat tcctacgcag gcctctcgat ccgcgagccg ccgcccgact ggcgtgggta
     2041 cgaggcattg caagtcgcgc tcgccaaccc cggtgcggcg ccgatccgca tcaacgtgcg
     2101 catcgacgat cagccgcgca atacggagta cgaggatcgc ttcaaccgcg agcgcacact
     2161 gcccgccggt gcgtcggtcg tctggcggat tccgatggac gaaatcgggc agggaccgaa
     2221 agggcgcagg cttgatctgt cggacatcag gcgggtcgtg atctttcttt caccgggctc
     2281 gcgcggcgcc tcattcgatc tcgggagcgt gcgtctcgag cgcgcgccct gataccctgt
     2341 gcgcccatgg atcagctcct tgcactcctg tcccagcccg ccgcctgggc ggcgctcgcc
     2401 acccttgtcg cgatggaggt ggtgctgggc attgacaacc tcatcttcat ctcgatcctc
     2461 acgaacaagc tgccagagca gcatcgcagc cgcgcgcggc gcatcggcat cgcccttgca
     2521 ctgatcctgc ggctcgcgct cctcggcacg atcgcgatca tcgtgcgcct cacgacgccg
     2581 ctgttcgaag ccctcggaca cggcttctcg tggcgcgatc tcatcctgat cgcgggcggt
     2641 ctgtttctcg tctggaaggc gaccaccgag atccaccaga gcgtcgatcc ggacgcggca
     2701 tctcacgtgc tggtgggttc agaggccacg atcggctttt cgtcggcaat catgcagatc
     2761 ctgttgctcg atctcgtgtt ctcgctcgac agcatcatca ccgctgtcgg catgaccgag
     2821 catattccga tcatggtgat cgcggtgatc gtcgcggtat tggcgatgct gctcgcggcc
     2881 gatccgctcg cgaagttcat ccacgacaac cccactatcg tgatgcttgc gcttggcttt
     2941 ctcctgatga tcggcatgac cctgatcgcc gacggcttcg gcgcacacgt gccgcgcggc
     3001 tatgtgtacg cggcgatggc gttttcagcc ttcatcgagg ggctcaacat gttcgcgcgg
     3061 cgcgccaagc ggcgacggcg gcaacggcga ttggccggtc aggcgcagac ccaaagcagc
     3121 agctgattgt tcgccggcat cgcgtgatca gcggcgagca cgaggccctc cgcacgcgcc
     3181 agcgcatcga ccgcttcgaa gtcgcggatg ccgcttgcag gatcacgcgc tttcagtgat
     3241 tcgtcgaagg ctgcattgct gtcagtcgtg aaagcacccc ggtatttgaa gggcccgtag
     3301 acggcgagca tacggcgagc gcaagcagcg gcgggctctc ccgccgctgc ttccatcggg
     3361 gatgacgcca gcaggcggcc gaccccggcg aagaacgcct cgacttcgcg ccacgccatg
     3421 atgtgcagcg tgttggcgga atagactgcg tccgcagtga cgaccggcca ccggggcatc
     3481 gagacatcga gttcgatcgg cgtggtgagg ttcccgggac cttcccgggc gatgcgcgcg
     3541 cgtaatgccg gcagccatgc cgcgcgatca ctcggctgcc acaggaggtg cgacagggcg
     3601 cgtgcaagaa acaccgcatg ctggccggtg ccgctgccga tctcgagcac gcgtcgggat
     3661 tgtgcgaagg ccggtccgat cacctcgagg atcggccgct tgttgcgctc gcttgcgggt
     3721 gaatccgggg taggatgcgc aggggctgga acaggggggg atttcgatga catggctcgt
     3781 gttcggtttg atcttgttcc tgggcgtgca ctcggtttcc attatcgcgc cggccgggcg
     3841 cgatcgcatg gcggcagcgc tcggcaacgg ctggcgggcc ctctattcga tcgcctcgat
     3901 cgtgggcttc ggcttgctga tctggggtta cggggtcgcg cgccaggaac ctgtccttct
     3961 gtaccagccc gcgccgtgga tgcgccacct cacgtcactg ctgatgctgc cggtgcttcc
     4021 gctgctgttt gcggcctacc tgccgggccg catcaaggcg aagttgcagc accccttgct
     4081 tgtcgcggtg aagctgtggg ccgttgcgca cctgctcgcg aacggcacgc tcgccgatgt
     4141 cgtgctgttc ggcagtttgc tcgcctgggc cgtgctcgac cgcatctcat tcaagagccg
     4201 agtgcaacgc ccgattccat cagcgccggc gagccgctgg aatgacctgg tcgcgatcgt
     4261 cctcggcctt gcggcgtacg tcgtcttcgc catggtcctg cacgtgcgct ggatcggcgt
     4321 gccagtcgtg ccttgaccgg gagtgcgttg aacacctttc gcgcgcttgg cttgcccgag
     4381 cctctgctcg ccgcgctcgc ggatgtcggc tacgaatcgc cgtcgccgat ccaggccgcg
     4441 accattccgg tgctgcttgc gggcagcgac ctcgttggac aggcgcagac aggcacaggc
     4501 aagaccgcgg cctttgcgct gccgctgctc gcgcgcctcg acctcgcaca gcgcgctccg
     4561 caggtgctcg tgctcacccc gacgcgggag cttgcgatcc aggtcgccga ggcgttccag
     4621 cgctatgccg cgcacctgcc agggtttcac gtgctgccgg tctacggcgg ccagggctat
     4681 ggcccgcaac tgcacgcgct gaagcgcggc acccaggtga tcgtcggtac cccggggcgt
     4741 gtgatggatc acatgaagcg cggcacgctc gtgcttggtg agctgcgcgc gctcgtgctg
     4801 gacgaagcgg acgagatgct gcgcatgggt ttcatcgagg atgtcgagtg gatcctcggc
     4861 gagacacccg aggcacgcca ggtggcgctt ttttcggcga cgatgccggc gccgatacgg
     4921 cgcatcgcgc agaagtacct gcgttcgccg gccgaggtga cgatcggggc gaagacgacg
     4981 accgcggcga acattcggca gcgctactgg atggtgagcg ggctgcacaa gctcgacgcg
     5041 ctgacccgta ttctcgaagt cgagcccttc gaaggcatgc tggtcttcgc gcggacgaag
     5101 caggcgaccg tcgagctggc cgagcgcctc gaggcacgcg gctttgcggc ggcagcgctg
     5161 aacggtgatg tgccgcagat gcagcgcgag cgcatcgtcg cgcagctgaa atcgggcgag
     5221 atcgacatcg tggtcgccac cgacgtcgcc gcgcgcggac tcgatgtcga gcgcatcggt
     5281 cacgtcgtga acttcgacgt gccgaccgat ccggaatcct acgtgcatcg catcgggcgc
     5341 acgggaaggg cggggcgcag cggcgaggcg atcctgttca ttgcgccgcg cgagcgcaat
     5401 ctgctgcgcg cgatcgagcg cgcaacgcga cagacgatcg agccgatgtc gctgccgacc
     5461 gtggaggatg tgaacgtgaa gcgggtcgcg cgcttcaggg aacgcatcgg cgcggcgctc
     5521 gaggcgggcg ccggggcgcc ttatcgggcg ctgatcgaga tgttcgcgcg cgagctggag
     5581 gttccggagg tggagatcgc cgcggcgctt gcggcgatga tgcaggggaa gacaccgctc
     5641 ctgctgcagc ccgaagcgcg ggaaagacgg gagcagcagg cgccgcgcga gcacgacccg
     5701 cgccccgcac gcgagcaccc cgcacgcgag cgtccggcac gagagggcac ggcgcgcaga
     5761 tcggcgcggc ttgccgacgt cgagatggag acgtttcgcg tcgaagtcgg ccgtgcgcac
     5821 ggcgtgcggc cggcgaatat cgtgggtgca atcgcgagcg aagcggggct cgccggcaag
     5881 cacatcgggc gcgtggacat ccgcggcgag cacagcttcg tcgatctgcc gggcggaatg
     5941 ccgaaagaga tcctgcgggc gctgcagaaa gtcatcgtga tggggcagcc gctgcgtatc
     6001 agcaggatga agcgcaaacc ccgggcatga gctgacggtc cggctattgg ctgctgctgg
     6061 tcagcaggaa ccctgcccaa tagtacggat gcctgtcagc gagcgacagt tgcgcgagct
     6121 gcagtgcgcg cgcgacatcg gtgcccgcag ccatctcgtc gtacatgcgg cgcatgagac
     6181 tcgccgtgct ctggtcctcg actcgccaga ggctcgcgat cacgctgctt gcgccccgcc
     6241 gctgcacgat cgcgctcaaa ccttcgatct cacggccgtc ggctgcgact gcgccaccca
     6301 tggcggtctg gcagccggag agtgtggcga gcgcaatgcc ggcaaagtcg agcgcgctga
     6361 tcttgtcgag cgtgaggcgc ccgccgtcgc ccagcagcag gaatgaccgc agcgcattgc
     6421 cggggcgcag gctgaagtgc gtgccgagat ggaggatcga gtaatcgtgc ggcccctcga
     6481 gcagctggcc aagccgcgcc tccgtgaacg cggcgttcgc gtagcccttg ccgggaagcg
     6541 cgccatcacc gatcgcaggc gccgggcatg ctttgcccgg gatctcgagg cccgtgatcg
     6601 ggccgcgcac gatgtcgcag agctcgtcgg cgactgcagg caatgcctga aagccgccca
     6661 cactgcgtgt cacaccgagt ccgaggacct cgaggtcggg tttcgtgccg tccgcctgcc
     6721 cgggctggcc ggcggcgtcg gcatacatct ggatcgcaaa gtcttcgacg aggtacttgc
     6781 gaccgtcatg gagcgcggcg aagggcacgt agcgcagcgc gtcgtcaggc cacagcacga
     6841 ggcgcttcgc gcttgccttg tgcgcggcct cgaccagcgg gcgcgcgagc aggtcgtaga
     6901 gcttgcggga gcgcacgcgt atgtcctctc tgcggctgat cgcgtcgagc aagtcgccga
     6961 tctcccggcg cagtgccggg gcgtctacct cgatgagatg ttcggtctgt tccgtgcggg
     7021 tggcgaccag gatgcgcaaa tgcctttcgg tcagcagata gacaccgatc gcgctttccg
     7081 ggccgaagcg ttgcagttcg cgtgcgaggc gcttcgtcgt gatgttccgc tgtgttgtgc
     7141 cgatgctgcc tccggtttca ctggcgacga actcgctgat gcgcttcgcc cgggcggctt
     7201 cgaccgcgtc ctggccctgg aggagctggc cgaggtggcg cgactcctgc ggagtgatac
     7261 gacctgcgtc gcgacggtgg gccagtgtgt cgatcgtcgc gcccgcctgc gcatccgcgc
     7321 gcagcagacg cgtgtatcgc gtcaggagct gccgctcgtc ttgggtcaat gccacgcggt
     7381 cctcatccgc ttcggctttc gtatccccgc gcaacacgaa atcgtacagt tcctgggctt
     7441 tcagcaggtc gaggacctgc agcccctcat ccatccggcc ggtctgcatc agccagtcgg
     7501 ccgttgcacg atagacgccg atcttgtcac gcaggaaccc ggcctgcagc tgctgggcgt
     7561 cctcgccgaa ccacgcgcgc agcttctcga tgctgtcaat cgcctgcttg ccgaaaaaga
     7621 tcgccagcga gtcgtcgcca cgatgatgca gcagcgtcgc gagccggtat tgcgcctgcc
     7681 acgccggatc gggcgtgccg agggtcgcgg cagcggacag ggcgcgcacc gcgtgcaacc
     7741 atgccgcgcc accgttgccg cgtgcttcgg cgagcgcgct cgcgttggcg tagaccgcgt
     7801 accgcaaccc gggctcgact tcacgcgcag cttcgaggcg ccgctgcgcg tcgaggcaat
     7861 tcggagcggt cgcgggcgcc gtgcgaatgt cgattccgca gaggtcgttg accacccgca
     7921 gcagcacagg atgggcggcg ggcaggcgcg cggcgtagag cgcgagtgcc tgcccgatct
     7981 gcgcacgcgc acgctctggt tcggtggtgg catcgaggtg ggcgagcgcg agcagcacgc
     8041 ccgcgcgttc gacggacagc gccgggtcgt cgcccgcaac cttcaggcct gcagcaagcg
     8101 acgcacgggc atcgtcgtac gcaccgagct ggtactggag caaaccgagg ccgcggtagt
     8161 gccgcacctg gacctgacgc tcgacgtctg cgccatgcgc tgcgatgagc tgcccggcgc
     8221 gtccgtagtg cgcgaatgct tgcggcattt cccccagcgt gcgttcgacc tcggcgagat
     8281 caagcagcac attcacgagg ttcggtgcgt ccgggccgag ctgctccacc gcgacatcga
     8341 gcgcgcgccg tgcaacgggc agggcttcct gcggcttgcc gcgcgcgccg agcagcgcgc
     8401 cgaggtgggt gagcgcaatc cgatagtgcc gctgcagcgg gccatcggta gcctctgcga
     8461 gccgcactgc ctcgcgcagc gcgctctccg cctccgcgta ctcatcgagc ttctcgagca
     8521 cctgcccgta gagcgtgtag gccgtcacgg gctgagccgg cccgcgctgt gcgcgcagcg
     8581 cgagttcgat cttcagctgc acctcggcat gccgccgcgc ggcaggataa tcctcttcgc
     8641 gcatcgcaat gttggtgagc gtattctcga tcgcgccggc agcttcgctg ctcgtttcgc
     8701 cgtttgccgc gagtattgcc tgcgcgcgct cgagcagcgt ccgcgcgtcg gcgaaccgcc
     8761 gctgacccac gcgcacctgt gcgagcccta cgagcgcctt ctgcaattcg atctgccttg
     8821 cacccggcac gctctcgcgg atatgcagcg cacgctccca ttcatcgccc gcgtctgcga
     8881 gacggccctg ccggaacgca atccacgcgt atgcgacgtg gatctcggcg atctccggcg
     8941 cgttcggccc gagccgccgt tcgtagatcg gcgtgacgaa gcggagcagc gggattgcct
     9001 cggcgaattc gtccatgtag gcgatcaacg gggttgcgag cgtctgcacc caccatgcga
     9061 attcgagcga gtcggcgccg tattcgcgct cggcgcgcgg gagggtcgag cacaggatgc
     9121 gcactgccgt catggggtcg ctttcaccga ccttctcgac ctgctgccgc aactcggtga
     9181 gtggcatcgc ggtgccctcg aacgatgcct gcttcgcgca ggcaggcggc agttcgatcg
     9241 ccgctgccgg tgcacacggc gctatggccg cgaggagcaa cagtgcgaat gcccgggcca
     9301 tcacgagccc gggcgggggg cgatctcgat gcgcagcacg ctgtccccag gaggcggaac
     9361 tccgaagcgt tcaagcacgg tacgcacggc gggtgtaagc gggcgcggca agtcggcatc
     9421 gagccctgcg cggccgaagc gctcgtagct cgctggctcg acgccggcat cgcgcagtgc
     9481 cgagacaatc tggctgcgca gcgccgcggg atcgtcggcc accatgcgat ggatggcatc
     9541 aggtgcgccg cgcatgatcg gagcctcgac ggccgggcgc agcgtccaga cgagcgccac
     9601 ggcgaggccg gcaatgccgg ccgcgagcaa cgcgccccag cgcccggtga gcttccgtgg
     9661 cgcggtgccg ctggtgcgct tgagcgccgg cgcgagaatc tcatcgcgcc gggcgcgctc
     9721 gagcagcagt gctgcgcgag tctcctcgtc gcggccaatc tcgcgctgca gcgacagttc
     9781 gtcggctcgc gtgcgcgcgt ggagcgcggc gcgcagcgcg cgcgcctcgt tgagcgtcgc
     9841 ggcgcgggcg ccctccgcac gcacgcctgc ggtctcgcgg cccgcgagcg cgtcgcgccc
     9901 cgcgagcgcg tcgagccagg cctgatcgtc agcctggtcg ctcatgacgc ttgcgccccg
     9961 aaggcgagct gataccactc ggcgagatac acgcgggcct tcttgcgaca ctgcgatatg
    10021 aactcgcgcg tcgcgcccgg tgtgcggccc aggagttgcg cgatcgcttc gttgtcgagc
    10081 ccgtcctcgc cgatccaggc gatcaccgcc gcgtgcgtcg ggcaatcccg ttcgaacgct
    10141 gcccagcagc gctgaaatac ctcgcgctgt tgccgcacct gaacctcgtc gagcggagtg
    10201 gagacggcgc gcgccgaaag ctcgaggatt ttctgctcga cttccgcggt cagcacggcg
    10261 tcgtccagtg agaccgcccg gtcgtggcga cggaaactgt cgagcatcgt gtgtcgcagg
    10321 atcgcccgca accacggcaa cagttccgaa ctgccgtgaa agctcgcgca gcgctgccag
    10381 accttgatga aagtttcctg caccagatcg cgcgccgcgg cggcgtcgcg cacggtgcgg
    10441 cggctctcac ggtagagcac ggcaaaatgt tcgcgatcga gcgtacgcat ggcctgctcg
    10501 agggcgctgc cgccgtcgcg gcaggcccgc atgaattgat ctttgtccaa tcgggtcccc
    10561 ggtacagcgt accggagtcg cgttgcagtg tccgcatgga atcaaagacg agtccggggg
    10621 cgggttgtca ggcagtcggg tcctgacaaa tccgggcggg ttgcgtcttg tgagtcgtgg
    10681 acacacggga aggacacagg gggctgccag atgaaaagca tgcttgcatt gctcgccgct
    10741 tgcggcgggc tcacggcttg tgatcagggg cgctggatcg agttgccggc gatgctgatg
    10801 acctgcaacg atctcgacga caagcagatg accgctgaac tcgcgaaccc cgacaagttc
    10861 agcctgggtc gcggcatgct gcaaggtgtg tgcacgcaga gcggggcggg gcagttcaca
    10921 ggcgagaccc gctgccagaa ggaagcagtt gaaatccggt gcaagtcatg agcgcgcggc
    10981 attgcgcgag cgcggggtat ctcatggccg ccctggcagc tctgtcgatc gccggctgcg
    11041 gtggctcgcc atccgggtcg ggcaacaagg atgcggcgac cctgccaacc tcgcgaggcg
    11101 atgtctggac aattgaccct gcgtcgccac cggatgcgca ggtgctcgcc ttcctccacg
    11161 gggtgcacaa cttcgtgctg aagggcgacg aaatctacac cgcaaccacg cggctcgatc
    11221 cggcacccgc cgccggtgcc gtggcggccg caacgctagc aggcgggctc gcgctgcata
    11281 tcgaggcgcc gggcgggcaa ccgatccttg tcttctcgag tggtcagcgc gcgccgctgc
    11341 gcaagttcca ggcaccggag ggcagccgat gaaagcgcat ctcactgggt tcgccctgtc
    11401 attcacgttg ctcatcggtt tgacctgtgc cggcggcgcc caggccgccg ggcgcgactt
    11461 cgacccggag cagctcgcgc tcacctatca caagctcgcc ggcaagccct tcgatttcga
    11521 tcgctatgcc gcctcgtcga acgtcgtggc gcgagcgtcc ggcttcgatc ggcccgacgt
    11581 gctgaagcag gaggtcgcgc gggtgcgtgc ggtcttcgac gcgggcagcg acagcatcat
    11641 cttcgagacc gacatccaga actccatcag cgattacgac cacgacaagg gtgagttctc
    11701 gatcgcgatc ttcgagccgg gctcctacat gccggtctcg ttctacggcc agcagtatcg
    11761 cgtcgtcttc gcgaacgccg aggcggcacg cgcgatccgc atgccgaagg aagaggcacg
    11821 cgagttcgat cgcggcctca tgcgtgacgg cagccgcggc gtcgtgtcat ccgtgaagtt
    11881 ccgggtcgtc ggcgacggcg atccggccgg tgcggtggat ggccagaacg tcgtgcgcgc
    11941 cgagctcgta tcgacgaaaa cgaccgacac cgacggcacg gtcctctcca cgccggatct
    12001 ggccgcggcg ccggcgggcg ctgcgcctgt cgcggccttc gacgcgcgca ccatcgacgc
    12061 cgccggcatg cgcatcggcg tgagcgcaga ggacctgcag gcagcgatca cgcgcctcta
    12121 tggcaagccc gatcgcactc cccgcggacc tcgctccgac tacgacccgc gcttcgcggg
    12181 ggagatcgag ctcgatctga tgcgttgcat ctacgtgcag gggcagcggg atcacgagcc
    12241 tgtgcccggc gatatctgcc tgcgcgccta ctacgacaag gacggcatcg tgcgccaggt
    12301 gcggatacaa cgtgtgctgc cgcgcttcga ttatgagcag gcgcgcgccg cggcgatcgc
    12361 gcgtttcgga cccgcggctg ccttgcgggg cggcaatacg ccggagcttt cctggggaag
    12421 caaggatccg aaactcggtc cggcgctggt gctcaggatc gatccttacg gcaacacgct
    12481 cagcttcggg tacagcgagg tgcgtgccgg ttcgctcgac ctgacgttga ccgatgtcgc
    12541 gtgggccgcc gccaccgccg ccctgtccgc caattgagga gtcgttcgat gaaactgcag
    12601 atactggccg cttccttgat cgcagggctg gcgatgacgc tcacgacacc ttcatttgcc
    12661 gccgccggcg gcgcctgcgc cggcagggcg cattgcgagg agacccggtc gttcgtcgcg
    12721 gacatgagca atttccgtgt cagcaagagc ggccgttacc gcgtgctgac cgcgaccatc
    12781 agcttccgca acaagaccgg caagcccctc gtgctcggtt atgtgaacga ttcaggtgtg
    12841 gccctcgacg agcgcggcaa tcgctatgtc gtcgcaaatc cgcaggcggt gcgcggcatg
    12901 ggagtcgtca cgggcagcag cttcgatccg aagttcgtgc tggcgccagg tgaagcggcc
    12961 gacacacgct tcgagctcat ctgggatccg ggcagggaga tcgtcggcgt cacctacgaa
    13021 ctcgacctcg ccgtgcgcga gatcaacgag gtggccggga accagtacaa gctcggggtg
    13081 gagtacgcgg tgcatttctc gcaactggcc gagggcggaa ccgcgtctgc cgtcgcgacc
    13141 accgccgcgc ctgccgctgc tgcgcccgcc gctgcggtgt ctgcaggtgg ggcgaccgac
    13201 ccgtgcggcg gctcgccacg ctgctacaac gcgggcgcgt ttaccgcaga gatcgcccag
    13261 ttgtccgcga ccgccatggg cagcggtgtg cgccaccact cgatatcgat caacatccgc
    13321 ttccgcaatg tcagcaacgc gccgatcgtt ctcggctaca agtccggttc gagcgcggcc
    13381 atcgacaact tcggcaaccg ctattactgg gggcgtgcag gcacccacga tacgagcgtc
    13441 aagggcatcg gtttcgtcag cggtcgcacg gcggacacgc agtttcagtt gcagcccggc
    13501 cagagccgca atgccacgtt caatctgatc cgctttgagg cgcgaccgcc gatcggcagc
    13561 gcctggggtt acgatgttgt gattgatgag ctcgagatcc tgccagggca acaagtacga
    13621 acggcccggg agaacagcct caatttccag agcctgacgg cccgcgcctt cgcgagcgcg
    13681 gc
//
LOCUS       DAORUQ010000086        13620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4404_length_13620_cov_5.873940, whole genome shotgun sequence.
ACCESSION   DAORUQ010000086 DAORUQ010000000
VERSION     DAORUQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4404_length_13620_cov_5.873940"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLW13_08995"
     CDS             complement(<1..195)
                     /locus_tag="PLW13_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70551.1"
                     /translation="MGHAWRFPLRVGFRYAGSSHRGALVAFISRVSMLGLVLGVALLI
                     VVLSVMNGFDRELRERILSLV"
     gene            complement(196..894)
                     /gene="lolD"
                     /locus_tag="PLW13_09000"
     CDS             complement(196..894)
                     /gene="lolD"
                     /locus_tag="PLW13_09000"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HQY70552.1"
                     /translation="MNDAVPGVPVLECRALRKCYREGPRELVVLDGVELNVARGERVA
                     IVGQSGSGKSTLLNMLGGLDLPSSGEVRVQGRVISALSERERGLLRNRALGFVYQFHH
                     LLGEFDALENVAMPLLIRGDAPAAAAASARALLTRVGLGERLEHKPGELSGGERQRVA
                     IARALVTEPRCVLMDEPTGNLDQGTAAEIHLLMDALNRDLGTSFVIVTHDPQLAARMD
                     RILTLARGRLHEGP"
     gene            complement(887..2128)
                     /locus_tag="PLW13_09005"
     CDS             complement(887..2128)
                     /locus_tag="PLW13_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQY70553.1"
                     /translation="MFRPFPLFIGLRYVRARRRTRFISVVSAVALLGLALGVMALLVV
                     LSVMNGFDRELRTRILSMVPHVLVHAAHPLQDWEPLATALEQDPAVVGAAPFIGGTAM
                     LSTPGVVRGVELTGIRPERDARVSPIGRYMVQGDLAQLRPGAFGVVIGNLLARSLGVQ
                     PGDHVTLMLPELSVTPAGAYPRMRRFRVVGIFAVGAQVDATTALIHLDDAARLYRTGG
                     GVQGVRLALADPDLAPAVRERVAPALAAGLVAEDWSVSQGSLFSAIRMEKRMVTLLLL
                     VIVAVAAFNIVSILTMVVAEKRGAIAVLRTMGASPRQVMAIFTVQGSLIGLAGLAMGL
                     VAGIPLAWHIGSVAGWIEQALGLRLFNPNVYFISYIPSQVRLADIVLIAGCGLALAVL
                     ATLYPALRASRIGPAEALRYE"
     gene            2262..2831
                     /locus_tag="PLW13_09010"
     CDS             2262..2831
                     /locus_tag="PLW13_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY70554.1"
                     /translation="MHERSANQRDFYRVDCHVMISHCAVGDHVPAGRQPDSYFPDGEH
                     FNLMRELRRMDHDASHLLHALGEKDRNLGAYLVHLNRKIDTLTRHLAALTPEMSRGSE
                     QVVSLSEGGVSFHAPQPPAPGSVLALRMTLLPAWVGIAVYGIVVAAGAGERNVAVNFE
                     QLQDADRQIIARHVMQVQMAEQRRAREGA"
     gene            2861..3187
                     /locus_tag="PLW13_09015"
     CDS             2861..3187
                     /locus_tag="PLW13_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70555.1"
                     /translation="MNAIARTLAAVAFGLAAAAAVTDELKIPVGSQAIAHAGPLPPHG
                     MLMPRVRAGWGEPALVHDAVGQPPITRWDYADFSVYFEHDHVVHAVAHHRRGDAPVSD
                     DQPTDD"
     gene            3189..4298
                     /locus_tag="PLW13_09020"
     CDS             3189..4298
                     /locus_tag="PLW13_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQY70556.1"
                     /translation="MLLILHPDIDESAEDYRKTCDFLRALPRIEMRTHVVQGQQQRLT
                     EIYLLGDTHALDKDEIEALPAVERVVRISEEYRMLGRHKDEKRQAGFEYNGVTFSQDT
                     LHVMAGLCAVDNPQNVETMLRALQQHGQVCTRMGAYKPRTNPYAFQGHGAKCLPWVFE
                     LAGKHGIRVIAMEVTHESHIEEIDTALEKLGRPTGVMLQIGTRNTQNFELLKAIGRQQ
                     TYPAMLKRGFGISLNESLNAAEYLASEGNSRVIFCLRGMKSDGGAPHRNMVDFAHVPV
                     IKRLTRMPVCVDPSHSVGSREAAPDRILDVAHATAQGVIAGANLVLVDFHPQPREALV
                     DGPQALLLDELPRFLDDIAACRETYERRRRIWNAC"
     gene            4292..5050
                     /locus_tag="PLW13_09025"
     CDS             4292..5050
                     /locus_tag="PLW13_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQY70557.1"
                     /translation="MLIYGHRGARGEAPENTLAGFHRAIAAGAPRVELDLRLSSDEEL
                     VVIHDETVSRTTDGRGLVAHKSVAELARLDARRAGPAWPDPQPVPTIARVLEEFPELQ
                     HLQLEAKPVDAGNRALMADRLSALFHDFDFHARATVTSFDRELLRAVRAADPRIALGL
                     VTDRARPEPLKTALALGARLLVLNWKLCTATRVQAAQAAGLQVSAWTVNDDAEARRLH
                     ALGIDSIVTDFPTRMVALLHGLGAGTRHHPRGAS"
     gene            complement(5090..7531)
                     /locus_tag="PLW13_09030"
     CDS             complement(5090..7531)
                     /locus_tag="PLW13_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQY70558.1"
                     /translation="MRHASAFAATVIAALALLSAGAGHAGGNKFRYDADVARTSYGIP
                     HIRAADWGSLGYGYGYVFAEDNACVLARDVLVATGTQSRWFGAGTNDANLYSDRVYRM
                     VNSDARSEAAWARLDADTRALLTGYADGYNRYLRDTGGELLAADCRAQPWVRPLAGLD
                     VLKVLRKLLVRAGTGSFVTQVATATPPAVPVAQAAGTTAPAALLAAAGPADAAAPLID
                     PADLPDFSPERFGSNAVGLGGELTGGAGALLGNPHFPWAGIERFYAVHLTIPGRYDVM
                     GGSIYGFPLVNIGFNRDVAWSHTVSTARRFVLRELALVPGNPRAYRYDGADIEMQPEA
                     VTVEVLQPDGSIATDPHTFWQTQFGPVVEPSPLIPWSLSTAYALTDVNLENDRAFRQY
                     IEMGQARDLGELRSALERHVGLPWVNTIAADSSGNAFYGDIGSIPNVTNAQLASCSKS
                     PIAAYLATLRVYTLDGSTSACDPGTDADAPVAGVFGAANLPGLMRRDFVQNSNDSYWL
                     ANPAERLEGFSSIIGADENRAQGFRTRLGLRQIQDRDAGIDALPGTGFDRRWLQDVLF
                     GNRHYSAEIMRPGVLRLCAATPPDVVIGEQSVNVASACQVLRDWDGRNDVASVGAHVW
                     GELWRRVSGAPTAGSGLPSVTNVLYAVPFDPADPVNTPRELNLASAEVATRVMGELAY
                     TVKFFADAGIPLTARWGDVQFEVRNGERIPIHGGSGTSGVYNAITTAPRVQDGGLTPV
                     VAGSSYIQAVTFRRRGPEAHAIVTYSQSTDPANPHFADMTALYSRERWITLPYNEGDI
                     SSDPNFVSFRLRERN"
     gene            complement(7657..9150)
                     /locus_tag="PLW13_09035"
     CDS             complement(7657..9150)
                     /locus_tag="PLW13_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY70559.1"
                     /translation="MSAAGTGLLGKLVGQTAIYGLSTVVGRLLNYLLVPLYTYQFTDP
                     ADFGVVTEFYAYASLLNVVLTYGMETAFFNFSAKRGRNPAVYSTALLSLGISSALFLA
                     FLTLNAQPLADALRYPEHPQYVLWMAWVIGLDALAAIPFARLREQQRAGRFALLKTLN
                     ILVNIGGNLLFLGLGKWAFESDTVPAWLGWAATLYDPQIGIGYVFIANLVASVVTLLL
                     LAPEFLQARARPELALWRQMLVYALPLLLAGLAGMVNETMDRILLKHLLPAQVAMQQL
                     GIYGACYKISILMSIFIQAFRYAAEPFFFAQYRQADARLLYALVMRWFVIACSFIFLA
                     VMANIAWVQYFVGASYREGLGVVPILLLANLCLGVFFNLSIWYKLTEQTRFGTYLALW
                     GAALTLALNFWWIPRLGYMGAAWATLICYASMALLSYLLGQKHFPVPYDLRRLLGYPL
                     LAVLLYACLLPIEGLAPAPELAARNGVLAAFLGMVFLLERKRRPSSG"
     gene            complement(9182..9403)
                     /gene="nqrM"
                     /locus_tag="PLW13_09040"
     CDS             complement(9182..9403)
                     /gene="nqrM"
                     /locus_tag="PLW13_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HQY70560.1"
                     /translation="MTTLLFTLGFFLLMALGMAVGLLAGRGPIKGSCGGMSAMSGEDC
                     PICGGNPARCDANDAASGELGYDATRERR"
     gene            complement(9400..10404)
                     /locus_tag="PLW13_09045"
     CDS             complement(9400..10404)
                     /locus_tag="PLW13_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQY70561.1"
                     /translation="MFLLAACAPEPEVWRFEGATMGTTWHVTLVDPPAGLAREAASAG
                     IEAELAGVNARMSTYSPDSEVSRFNHAAPGEWFAVSADTAAVVATALEVHQGSGGAFD
                     ITVGPLVDLWGFGAGSKATDRVPSDAEVAATAARVGSDALRVRADPPALFKSADREID
                     LSAIAKGFGVDRAALWLDGNGVANYMVEVGGEVRTRGRNPQGEKWRIGIEAPELLQQG
                     HAITAVALSGESVATSGDYRNFFEAGGRRYSHTIDPATARPVEHALASVTVVAADCKT
                     ADALATAIDVLGPEKGLAFAEREKLAVYLLVRAGTGFESRHSPAFAPFLGTAAAKEAP
                     "
     gene            complement(10457..11692)
                     /gene="nqrF"
                     /locus_tag="PLW13_09050"
     CDS             complement(10457..11692)
                     /gene="nqrF"
                     /locus_tag="PLW13_09050"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HQY70562.1"
                     /translation="MVDTGTIFWGVGMFTAIVMSLAVLILLARSKLVSTADVTIEIND
                     DPSRALTVEAGGKLLQTLANSGIFLSSACGGGGTCGQCRCQVLDGGGTFLPVEEGHFS
                     RGQLREHWRLACQVAVKQDMKIHVEPEFFGVKQWDCQVVSNDNVATFIKELVLKLPEG
                     ESVDFRAGGYVQLEAPAHTVFYRDFDVAPEYRGDWERFKFFELSSTVEEPVIRAYSMA
                     NYPDERGLLKFNIRIATPPPGSKGIPPGKMSSYVFNLKPGDRMRVFGPFGEFFAKDTR
                     AEMIFIGGGAGMAPMRSHLFDQLKRIHTDRKISFWYGARSLREMFYVEDFDGLAATNP
                     NFSWHVALSDPQPEDNWTGLKGFIHQVLFDNYLKDHPAPEDCEYYMCGPPMMNAAVIK
                     MLEELGVERDNILLDDFGG"
     gene            complement(11718..12326)
                     /gene="nqrE"
                     /locus_tag="PLW13_09055"
     CDS             complement(11718..12326)
                     /gene="nqrE"
                     /locus_tag="PLW13_09055"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HQY70563.1"
                     /translation="MEHYLALLIRSVFVENMALAFFLGMCSFIAISKKVETAFQLGVA
                     VVVVQTITVPANNLIFHYLLRDGALSWAGFPELDLSFLGFLSYIGVIAAIVQILEMFL
                     DKYMPALYHALGVFLPLITVNCVIMGGSLFMVERDYMFGESVVYGFGSGLGWALAITA
                     LAGVREKLKYSDVPQGLQGLGITFITVGLMSLGFMSFGGISL"
     gene            complement(12326..12988)
                     /locus_tag="PLW13_09060"
     CDS             complement(12326..12988)
                     /locus_tag="PLW13_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HQY70564.1"
                     /translation="MTKTREILVSPIFSNNPIAVQILGICSALAVTSSLKTALVMSIA
                     LTLVTAFSNLLISLLRNHTPSSIRMIVQMIVISSLVIVVDQILQAVAYDISKQLSVFV
                     GLIITNCIVMGRAEAFAMKNPPIPSFIDGIGNGLGYSAMLMAVGTIRELLGSGSLFGF
                     PVLETVNNGGWYQPNGLLLLPPSAFFIIGGFIWLLRSWKKHQVEPAEYRIEPQTKAQE
                     AV"
     gene            complement(12988..>13620)
                     /locus_tag="PLW13_09065"
     CDS             complement(12988..>13620)
                     /locus_tag="PLW13_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HQY70565.1"
                     /translation="EHGAAEIEELFGRFTVRMVDLDTGEYLSAEQLARPELAPERYDQ
                     IKAAKDPKLSEALPGDADIAGIKRREKVAQVYLLEDEGGLQKIVLPIRGYGLWSTLYG
                     FLVLEGDASTVIGLAYYDQKETPGLGGEVDNPKWKALWPGKQVFDAEGAVSIDIVKGS
                     VDRESPAAVHQVDGLSGATLTSNGVEHMLHFWLGARGFGPYLSRIRGGQA"
BASE COUNT         2298 a         4750 c         4615 g         1957 t
ORIGIN      
        1 caccagcgac aggatgcgct cgcgcagctc gcggtcgaag ccgttcatca ccgacagcac
       61 cacgatcagc agcgccacgc cgagcaccag gcccagcatc gagacccgcg agatgaacgc
      121 caccagcgcg ccgcggtgcg aactgcccgc gtagcggaaa ccgacccgca gcggaaagcg
      181 ccaggcgtgg cccattcagg gtccctcgtg caggcgcccg cgcgccagcg tgaggatgcg
      241 gtccatgcgc gcggcgagct gcggatcgtg cgtcacgatc acgaagctgg tgccgagatc
      301 gcggttcagc gcgtccatca ggaggtggat ctccgcggcc gtgccctggt cgaggttgcc
      361 ggtcggctcg tccatcagca cgcagcgcgg ctcggtcacc agcgcgcgcg cgatcgcgac
      421 acgctggcgc tcgccgccgg agagctcgcc cggcttgtgc tcgaggcgct cgcccaggcc
      481 gacgcgcgtc agcagcgcgc gcgcgctggc ggcggccgcg gccggcgcgt cgccacgtat
      541 cagcagcggc atcgcgacgt tttccagcgc gtcgaattcc ccgagcaggt gatggaactg
      601 gtagacgaag ccgagcgcgc gattgcgcag caggccgcgc tcgcgctcgc tgagcgcgct
      661 gatcacgcgc ccttgcaccc gcacctcgcc gctgctgggc aggtccagcc cgcccagcat
      721 gttcagcagc gtgctcttgc ccgagcccga ctgccccacg atcgcgacgc gctcgccgcg
      781 cgccacattg agctccacgc cgtcgagcac caccagttcg cgcggcccct cgcggtagca
      841 cttgcgcagc gcgcggcact cgagcaccgg tacgcccggc acggcatcat tcatagcgca
      901 aagcctccgc gggaccgata cgcgaggcgc gcagcgccgg atagagcgtc gccagcaccg
      961 cgagcgcgag cccgcagccg gcgatcagca cgatatcggc gagccgcacc tgcgacggga
     1021 tgtagctgat gaagtagacg ttggggttga acagccgcaa gcccagcgcc tgctcgatcc
     1081 agcccgcgac gctgccgatg tgccaggcca gcgggatgcc cgcgacgagg cccatggcga
     1141 gcccggccag cccgatcagc gagccctgca ccgtgaagat cgccatcacc tggcgcggcg
     1201 aggcgcccat ggtgcgcagc accgcgatcg cgccgcgctt ctcggcgacg accatcgtga
     1261 gtatcgagac gatgttgaag gccgcgaccg cgacgatcac cagcagcagc agcgtgacca
     1321 tgcgcttctc catgcggatc gcgctgaaca ggctgccctg cgagacgctc cagtcctcgg
     1381 ccaccaggcc cgcggcgagc gccggtgcga cgcgctcgcg caccgcgggg gcgaggtcgg
     1441 ggtccgcgag cgcgagccgc acgccctgca cgccgccgcc ggtgcggtag agccgcgcgg
     1501 catcgtcgag gtggatcagc gcggtcgtcg catcgacctg cgcgccgacc gcgaagatgc
     1561 ccacgacccg gaaacggcgc atgcgcggat acgcgcccgc cggcgtcacg ctgagctcgg
     1621 gcagcatcag cgtgacgtgg tcgcccggct gcacgccgag cgagcgcgcc agcaggttgc
     1681 cgatcacgac gccgaaggca ccggggcgca gctgtgccag atcgccctgc accatgtagc
     1741 gcccgatcgg cgagacgcgc gcgtcgcgct cgggccggat gccggtcagc tcgacgccgc
     1801 gcaccacgcc gggcgtgctc agcatcgcgg tgccaccgat aaagggcgct gccccgacca
     1861 cggcaggatc ctgctcgagc gcggtggcga gcggctccca gtcctgcagc ggatgcgccg
     1921 catgcaccag cacgtgcggc accatcgaga ggatgcgcgt gcgcagctcg cggtcgaagc
     1981 cgttcatcac cgacagcacc acgagcagcg ccatcacgcc gagcgcgagc cccagcagcg
     2041 cgaccgccga caccaccgag atgaagcgcg tgcgccgccg cgcacgcacg tagcgcaggc
     2101 cgatgaacag ggggaagggt cgaaacatcg cggcatttaa gcacaaatgc gcgaggcggc
     2161 acacggcgtg cccggcgtgc aaattgcgcc gctttgtggt ggacggtacg gcgcttatac
     2221 tgacccggaa gccatcccgg gggttcgcgg agaccgctgt catgcacgag cgcagcgcca
     2281 accagcgtga tttctaccgc gtcgactgcc acgtgatgat cagccattgc gcggtgggcg
     2341 accacgtgcc cgccggccgc cagccggaca gttactttcc cgacggcgag cacttcaacc
     2401 tgatgcgcga gctgcgccgc atggatcacg acgccagcca cctgctgcac gcgctcggcg
     2461 agaaggaccg caatctcggc gcctacctcg tgcacctgaa ccgcaagatc gacacgctga
     2521 cacgccacct cgcggcactg acgcccgaga tgagccgcgg cagcgagcag gtcgtctcac
     2581 tgagcgaggg cggcgtgtcg ttccatgccc cgcagccgcc ggcaccgggc agcgtgctcg
     2641 cgctgcgcat gacgctgctg ccggcctggg tcggcatcgc ggtgtacggg atcgtggtgg
     2701 ccgcgggtgc gggcgagcgc aacgtcgcgg tgaacttcga gcagttgcag gatgccgacc
     2761 gccagatcat cgcgcgccac gtgatgcagg tgcagatggc cgagcagcgc cgcgcccgcg
     2821 aaggcgcctg agcggcgcgc cacacgcaca ggagattcgc atgaacgcta tcgcccgaac
     2881 gctcgccgcc gttgccttcg gcctggcggc agcagccgcc gtgaccgatg agctgaagat
     2941 tcccgtcggc tcgcaggcga tcgcccacgc gggtccgctg ccgccgcacg gcatgctgat
     3001 gccgcgcgtg cgcgccggct ggggcgaacc cgcgctggtg cacgacgcgg tgggacaacc
     3061 gccgatcacg cgctgggact atgcggattt ttcggtgtat ttcgagcacg accacgtcgt
     3121 gcacgcggtg gcgcatcacc gccgtggcga cgcccccgtt tccgacgatc aaccaacgga
     3181 cgactgatat gctgctgata ctccaccccg atatcgacga atccgccgag gactaccgca
     3241 agacctgcga cttcctgcgc gcgctgccgc gcatcgagat gcgcacgcac gtggtgcagg
     3301 gccagcagca gcgcctcacc gagatctacc tgctcggcga cacccacgca ctcgacaagg
     3361 acgagatcga ggcgctgccc gcggtggagc gcgtggtgcg catctccgag gagtaccgca
     3421 tgctcgggcg ccacaaggac gagaagcgcc aggcgggctt cgagtacaac ggcgtcacct
     3481 tctcgcagga caccctgcac gtgatggcgg gcctgtgcgc ggtcgacaac ccgcagaacg
     3541 tcgagaccat gctgcgcgcg ctgcagcagc acgggcaggt gtgcacgcgc atgggcgcct
     3601 acaagccgcg caccaacccc tacgccttcc agggtcacgg cgcgaaatgc ctgccgtggg
     3661 tgttcgagct cgccggcaag cacggcatcc gcgtgatcgc gatggaggtc acgcacgaga
     3721 gccacatcga ggagatcgac accgcgctcg agaagctcgg gcgccccacg ggcgttatgc
     3781 tgcagatcgg cacgcgcaac acgcagaact tcgagctgct gaaggcgatc gggcgccagc
     3841 agacctatcc ggcgatgctc aagcgcggct tcggcatcag cctgaacgaa tcgctgaacg
     3901 ccgccgagta cctcgcctcc gagggcaact cgcgcgtgat cttctgcctg cgcggcatga
     3961 agagcgacgg cggcgcgccg caccgcaaca tggtcgattt cgcgcacgtg ccggtgatca
     4021 agcgcctcac gcgcatgccg gtgtgcgtcg atccctcgca ttccgtgggc tcgcgcgagg
     4081 cggcgcccga tcgcatcctc gacgtggcgc acgccaccgc gcagggcgtg atcgccgggg
     4141 cgaacctggt gctggtggac ttccacccgc agcccaggga ggcactggtg gacggaccgc
     4201 aggcgctgct gctcgacgag ctgccgcgct tcctcgacga tatcgcggcc tgccgcgaga
     4261 cctatgagcg ccgccgccgg atctggaacg cgtgctgatc tacggccatc gcggcgcgcg
     4321 cggcgaggcg cccgagaaca cgctcgcggg cttccaccgc gcgatcgccg ccggcgcgcc
     4381 gcgcgtggag ctggacctgc gtctttcctc ggacgaggag ctcgtggtga tccacgacga
     4441 gaccgtgagc cgcaccacgg acgggcgcgg attggtcgcg cacaagagcg tcgcggaact
     4501 ggcgcgcctc gatgcgcgcc gcgcgggacc ggcgtggccc gatccgcagc cggtgccgac
     4561 gatcgcgcgg gtgctggagg agttccccga actgcagcac ctgcagctgg aagccaagcc
     4621 cgtcgatgcg ggcaaccgcg ccctgatggc cgatcgcctg agcgcgctgt tccatgactt
     4681 cgatttccac gcacgcgcga ccgtgacctc cttcgaccgc gagctgctgc gcgccgtgcg
     4741 cgcggcggat ccgcgcatcg cgctcggcct ggtcacggac cgcgcgcggc ccgagccgct
     4801 gaaaacggcg ctggcgctgg gcgcacggct gctggtgctc aactggaaac tgtgcacggc
     4861 aacgcgcgtg caggccgcgc aggcggcagg gctccaggta tcggcgtgga ccgtgaacga
     4921 cgacgccgag gcgcggcgcc tgcacgcgct cggcatcgac agcatcgtga ccgacttccc
     4981 gacgcgcatg gtcgcgctgc tgcacggcct cggcgcaggc acccgacacc acccgcgcgg
     5041 cgcgtcctag gcgcgggccc ggcacgcgga ccgcgccggt ccggtccggt cagttgcgct
     5101 cgcgcagccg gaacgaaacg aagttcggat cgctgctgat gtcgccctcg ttgtacggca
     5161 gggtgatcca gcgctcgcgc gagtacagcg cggtcatgtc cgcgaagtgc ggattcgccg
     5221 gatcggtgga ttgcgaatac gtcacgatcg cgtgcgcctc gggccccctg cgcctgaacg
     5281 tgacggcctg gatgtagctc gaaccggcga cgacgggcgt gagccccccg tcctgcaccc
     5341 gcggcgcggt cgtgatcgcg ttgtacacgc cggaggtgcc cgaaccgccg tggatcggga
     5401 tgcgctcgcc gttgcgcacc tcgaactgca cgtcgcccca gcgcgcggtg agcgggatgc
     5461 cggcgtcggc gaagaacttc acggtgtagg cgagctcgcc catcacgcgc gtcgcgacct
     5521 cggcactggc gaggttgagc tcgcgcggcg tattgaccgg atcggcggga tcgaagggca
     5581 cggcatagag gacgttcgtg accgacggca gccccgagcc cgccgtcggc gcgccggata
     5641 cccgccgcca cagttcgccc cagacgtgcg cgcccacgct cgcgacatcg ttgcgcccgt
     5701 cccagtcgcg cagcacctgg caggcgctcg cgacgttcac gctctgttcg ccgatgacga
     5761 catccggggg cgtcgccgcg cacaagcgca ggacgccggg gcgcatgatc tcggcgctgt
     5821 agtgccggtt gccgaacagc acgtcctgca gccagcggcg atcaaagccc gtgccgggca
     5881 gcgcgtcgat gccggcatcg cgatcctgga tctggcgcag gccgagccgc gtgcggaatc
     5941 cctgcgcgcg gttctcgtcg gcgccgatga tgctggagaa accctcgagc cgctcggcgg
     6001 gattcgccag ccagtagctg tcgttggagt tctgcacgaa gtcgcgccgc atcagccccg
     6061 gcaggttggc ggcgccgaac actcccgcga ccggcgcatc ggcgtcggta cccggatcgc
     6121 aggccgaggt cgagccatcg agcgtgtaca cgcgcagcgt ggcgaggtag gccgcgatcg
     6181 gcgatttcga gcagctggcg agttgcgcgt tggtgacgtt cgggatcgag ccgatatcgc
     6241 cgtagaacgc gttgccgctg ctgtcggcgg cgatcgtgtt cacccacggc aggccgacgt
     6301 gtcgctccag cgccgagcgc agttccccca ggtcgcgcgc ctgccccatc tcgatgtact
     6361 ggcggaaggc gcgatcgttc tcgaggttga cgtccgtcag cgcgtaagcg gtgctcagcg
     6421 accacgggat cagcggcgag ggctcgacga ccgggccgaa ctgtgtctgc cagaacgtgt
     6481 gtgggtcggt tgcgatcgag ccgtcgggct gcagcacctc gaccgtgacc gcttccggct
     6541 gcatctcgat gtcagcgccg tcgtagcggt aggcgcgcgg gtttcccggg accagcgcca
     6601 gctcccgcag cacgaagcgc cgcgcggtcg agacagtgtg gctccaggcg acatcgcggt
     6661 tgaagccgat gttgaccagg gggaagccgt agatcgagcc ccccatgacg tcgtagcgtc
     6721 ccggaatcgt gaggtgtacc gcgtagaagc gctcgatgcc ggcccacggg aaatgcgggt
     6781 tgccgagcag cgcgccggcg ccgccagtaa gctcgccgcc gagccctacg gcgttgctgc
     6841 cgaaacgttc cgggctgaag tccggcagat cggccggatc gatcagcggc gccgccgcgt
     6901 ccgcggggcc tgccgcggcg agcagggcgg cgggcgctgt cgtgccggcg gcttgcgcca
     6961 ccggcacggc aggaggcgtc gcggtcgcga cctgagtgac gaaactcccg gtgccggcgc
     7021 gcaccagcag cttgcgcagc accttgagca cgtcgagccc cgcgagcggg cgcacccagg
     7081 gctgcgcgcg acaatccgcg gccagcagtt cgccgccggt atcgcgcagg tagcggttgt
     7141 agccatcggc ataaccggtc agcagcgcgc gcgtgtcggc atcgagtcgc gcccacgcgg
     7201 cctcgctgcg cgcatccgag ttcaccatcc ggtagacgcg atccgaatac aggttggcgt
     7261 cgttcgtgcc cgcgccgaac cagcgcgact gcgtgccggt ggccaccagc acgtcgcgcg
     7321 cgagcacgca ggcgttgtcc tcggcgaaca catagccgta accgtagccg aggctgcccc
     7381 agtcggcggc gcggatatgc gggatgccgt agctcgtgcg cgcgacatcg gcgtcgtaac
     7441 ggaacttgtt gccgccggcg tggccggctc ccgcgctcag cagggccagc gcggcgatga
     7501 cggttgcggc gaatgcggat gcgtggcgca cgatgccccc tcgggttctt gtggttatgt
     7561 cctcgatccg ccccggcacg gcccgggcga attggcccga gtctaagccg gtggccgcgc
     7621 cgacgtccat tcccgggtgc gcgacggatc tcgtgatcag ccgctcgagg gccggcgctt
     7681 gcgttccagc aggaacacca tcccgagaaa cgccgccagc acgccattgc gcgccgccag
     7741 ttccggggcc ggagccaggc cctcgatcgg caacaggcac gcataaagca gcaccgccag
     7801 caacgggtag ccgagcagcc gtcgcaggtc gtagggcacc gggaagtgct tctgccccag
     7861 caggtacgac aacagcgcca tgctcgcgta gcagatcagc gtggcccagg ccgcgcccat
     7921 gtagcccagg cgcgggatcc accagaaatt cagtgccagc gtgagcgccg ccccccacag
     7981 ggccaggtag gtgccaaagc gcgtctgctc cgtgagcttg taccagatcg agagattgaa
     8041 gaagacgccc aggcagagat tcgccagcag caggatgggc accacgccca acccctcgcg
     8101 gtagctggcg ccgacgaaat actgcaccca ggcgatgttc gccatgaccg cgaggaagat
     8161 gaacgagcag gcgatgacga accagcgcat caccagcgcg tacagcagcc gggcatcggc
     8221 ctgccggtac tgggcgaaga agaacggctc ggcggcgtag cggaaggcct ggatgaatat
     8281 gctcatcagg atcgagatct tgtagcaggc gccgtagatg cccagctgct gcatcgcgac
     8341 ctgcgccggc agcaggtgct tcagcaggat gcggtccatt gtctcgttca ccatgccggc
     8401 cagcccggcc agcagcagtg gcagcgcgta caccagcatc tggcgccaca gcgcgagctc
     8461 gggccgcgcg cgcgcctgca ggaactccgg cgccagcagc agcagcgtga cgacgctcgc
     8521 cacgaggttg gcgatgaaca cgtagccgat gccgatctgc gggtcgtaga gcgtggccgc
     8581 ccacccgagc cacgccggca ccgtatccga ctcgaaggcc catttgccca gcccgaggaa
     8641 gagcaggttg ccgccgatgt tgacgaggat gttcagggtc ttcagcagcg cgaaacgccc
     8701 ggcgcgctgc tgctcccgca gccgcgcgaa cggtatggcc gccagcgcat cgagaccgat
     8761 cacccaggcc atccacagca cgtattgcgg gtgttcgggg tagcgcaggg cgtccgccag
     8821 gggctgcgcg ttcagcgtca ggaaagcgag gaacaacgcg ctgctgatcc cgagcgacaa
     8881 cagcgcggtg ctgtagaccg cggggttgcg gccgcgcttg gccgagaaat tgaagaacgc
     8941 cgtctccatg ccgtaggtga gcaccacgtt cagcagggag gcgtaggcgt agaactcggt
     9001 gacgacgccg aaatccgcgg ggtcggtgaa ctggtaggtg taaagcggga ccagcaggta
     9061 gttgagcagc ctgcccacca cggtgctgag gccgtagatc gcggtctggc cgacgagctt
     9121 gccgagcagc ccggtgcccg cggcgctcat gagagctctc cggcccgggg agccgcggcg
     9181 ctcaacggcg ctcgcgcgtg gcgtcgtagc cgagctcccc ggacgcggcg tcgttcgcgt
     9241 cgcaccgggc gggattgccg ccgcagatcg ggcaatcctc gcccgacatc gcgctcatgc
     9301 cgccgcagct gcccttgatc gggccgcggc cggcgagcag gccgaccgcc atgcccagcg
     9361 ccatcaacag gaagaaaccc agcgtgaaca acaacgtggt catggcgcct ccttggcagc
     9421 ggcagtcccg agaaacggcg cgaaggccgg gctgtggcgc gattcgaagc ccgtgcccgc
     9481 gcgcacgagc aggtacaccg ccagcttctc gcgctcggcg aaggcgaggc ccttctccgg
     9541 gccgagcaca tcgatcgcgg tcgccagcgc atcggccgtc ttgcagtccg ccgccacgac
     9601 ggtcaccgag gccagcgcgt gctcgacggg gcgcgcggtc gccgggtcga tggtgtgcga
     9661 atagcgcctg ccgccggcct cgaaaaagtt gcggtaatcg ccggaggtcg cgaccgattc
     9721 gccgctgagc gccaccgcgg tgatcgcgtg cccctgctgc agcagctcgg gcgcctcgat
     9781 gccgatgcgc cacttctccc cctgcgggtt gcgcccgcgg gtgcgcacct cgcctcccac
     9841 ttcgaccatg tagttcgcca cgccgttccc gtcgagccac agcgcggcgc gatcgacacc
     9901 aaagcccttg gcgatcgcgg acaggtcgat ctcgcgatcc gcagatttga acagggccgg
     9961 cgggtcggcg cgcacgcgca gggcgtccga gccgacgcgc gcggcggtgg ccgccacctc
    10021 cgcatccgac ggcacgcgat cggtagcctt cgatccggcg ccgaagcccc acaggtccac
    10081 cagcggaccg accgtgatgt cgaaagcccc gccgctgcct tggtgcacct cgagcgcggt
    10141 ggcgaccacg gcggcggtat ccgccgagac cgcgaaccat tcgcccggcg cggcgtggtt
    10201 gaagcgcgag acctcggaat ccgggctgta ggtggacatc cgggcgttca ctcccgcaag
    10261 ttccgcctcg atgccggcgg acgcggcctc gcgcgcgagc cccgcgggcg gatcgaccag
    10321 cgtgacgtgc caggtggtgc ccatcgtggc gccctcgaag cgccacacct cgggttcggg
    10381 cgcgcacgca gcgagcagaa acacgaacgg gagcagccag gctgctcccg ttcgccgcgg
    10441 tgcgatgcgc gccgtctcag ccgccgaagt cgtccagcag gatgttgtcg cgctcgacac
    10501 cgagttcctc cagcatcttg atcacggccg cgttcatcat cggcggaccg cacatgtagt
    10561 actcgcagtc ctcgggcgcc ggatggtcct tcaggtagtt gtcgaacagc acctggtgga
    10621 tgaaaccctt gagcccggtc cagttgtcct cgggctgcgg gtccgacagc gccacgtgcc
    10681 agctgaagtt cgggttcgtc gccgcgagac catcgaagtc ctcgacatag aacatctcgc
    10741 gcaggctgcg cgcgccgtac cagaacgaga tcttgcggtc ggtgtggatg cgtttcagct
    10801 ggtcgaagag gtgcgagcgc atcggcgcca tgcccgcgcc gccgccgatg aagatcatct
    10861 cggcgcgcgt gtccttggcg aagaactcgc cgaagggccc gaacacgcgc atccggtctc
    10921 ccggcttcag gttgaacacg taggacgaca tcttgcccgg cgggatgccc ttcgatccgg
    10981 gcggcggtgt cgcgatgcgg atattgaact tcagcagccc tcgctcgtcg ggatagttgg
    11041 ccatcgagta ggcccggatc accggctcct cgaccgtgga gctgagctcg aagaacttga
    11101 agcgttccca gtcgccgcgg tactccggag cgacatcgaa atcgcgatag aagaccgtgt
    11161 gcgccggggc ttccagctgc acgtagcccc cggcgcgaaa atcgacgctt tcgccctcgg
    11221 gcagcttcag caccagttcc ttgatgaagg tcgcgacgtt gtcgttggag acgacctggc
    11281 agtcccactg cttcacgccg aaaaactcgg gctcgacatg gatcttcatg tcctgcttca
    11341 ccgcgacctg gcaggccagg cgccagtgct ctcgcagctg cccacgggag aagtgcccct
    11401 cctccaccgg caggaaggtg ccgccgccgt cgagcacctg gcaacggcac tggccgcagg
    11461 ttccgccgcc gccacaggcg gacgagagga agatgcccga gtttgccagc gtctgcagca
    11521 gcttgccgcc ggcctcgacg gtcagcgcgc gcgagggatc gtcgttgatc tcgatcgtga
    11581 cgtcggcggt gctcaccagc ttggagcgcg ccagcaggat cagtactgcc agcgacatca
    11641 cgatggccgt gaacatcccc acgccccaga aaatcgttcc cgtatctacc atggtcgata
    11701 atcccgaagt ggcccggtta cagcgagatg ccgccgaagg acatgaagcc cagcgacatc
    11761 aggcccaccg tgatgaaggt gatcccgagc ccctgcagtc cctgcggcac atcgctgtac
    11821 ttgagtttct cgcgcacgcc ggcgagcgcc gtgatcgcaa gcgcccagcc gagccccgag
    11881 ccgaagccgt agaccacgct ctcgccgaac atgtagtcgc gctcgaccat gaacagcgag
    11941 ccgcccatga tcacgcagtt caccgtgatc agcggcagga acacgccgag cgcgtggtag
    12001 agcgccggca tgtacttgtc gaggaacatc tcgaggatct gcacgatcgc cgcgatcacg
    12061 ccgatgtagc tgaggaagcc gaggaaactc agatccagtt ccgggaagcc ggcccaggac
    12121 agtgctccgt cacgcagcag gtagtggaag atcaggttgt tggccggcac cgtgatggtc
    12181 tgcaccacca ccaccgcgac cccgagctgg aaggccgtct cgaccttctt cgagatcgct
    12241 atgaagctgc acatgccgag gaagaaggcg agcgccatgt tctcgacgaa cacggagcgg
    12301 atcagcagcg ccaggtaatg ctccatcaca cggcctcctg tgccttggtc tgcggctcga
    12361 tacggtactc ggccggctcg acctggtgct tcttccagct gcgcagcagc cagatgaagc
    12421 cgccgatgat gaagaacgcg ctgggcggca gcagcagcag gccgttcggc tggtaccagc
    12481 cgccgttgtt cacggtctcg agcaccggga agccgaacag cgaaccgctg cccagaagct
    12541 cgcggatggt gcccacggcc atcagcatcg cgctgtagcc cagcccgttg ccgatgccgt
    12601 cgatgaagct cgggatcggc gggttcttca tcgcgaaggc ctcggcgcgc cccatcacga
    12661 tgcagttggt gatgatcagc ccgacgaata ccgagagctg cttcgaaatg tcataggcca
    12721 ccgcctgcag gatctggtcg accacgatca ccagcgagga gatcacgatc atctgcacga
    12781 tcatgcggat gctcgagggc gtgtggttgc gcagcagcga gatcagcagg ttggagaacg
    12841 cggtgaccag cgtcagcgcg atcgacatca ccagcgcggt tttcaggctg gacgtcaccg
    12901 cgagcgccga acagatcccg aggatctgca ccgcgatcgg gttgttgctg aagatcggcg
    12961 ataccagaat ttccctggtc ttggtcatca ggcctgtcct ccgcgaatcc tgctcaaata
    13021 gggaccgaaa ccgcgcgcgc ccagccagaa gtgcagcatg tgctcgaccc cgttgctggt
    13081 gagcgtcgcg cccgagagcc cgtcgacctg gtgcacggcg gccggcgact cccgatcgac
    13141 gctgcccttg acgatgtcga tcgacaccgc gccctcggcg tcgaagacct gcttgcccgg
    13201 ccacaacgcc ttccatttcg ggttgtcgac ctcgccgccg agcccgggcg tttccttctg
    13261 gtcgtaatac gccaggccga tcaccgtgct ggcgtcgccc tccagcacca ggaagccgta
    13321 cagcgtgctc cacagcccgt aaccgcggat cggcagcacg atcttctgca gcccgccctc
    13381 gtcctcgagc aggtagacct gggcgacctt ctcgcggcgc ttgatgcccg cgatgtcggc
    13441 atcacccggc agcgcctcgg agagcttcgg atccttcgcg gccttgatct ggtcgtagcg
    13501 ctcgggggcc agttccggcc gcgccagttg ctccgcggac aggtactcgc cggtgtcgag
    13561 gtcgaccatg cgcaccgtga agcgcccgaa cagctcctcg atctcggcgg caccgtgctc
//
LOCUS       DAORUQ010000087        13367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4555_length_13367_cov_6.313627, whole genome shotgun sequence.
ACCESSION   DAORUQ010000087 DAORUQ010000000
VERSION     DAORUQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_13367_cov_6.313627"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLW13_09070"
     CDS             complement(<1..345)
                     /locus_tag="PLW13_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQY70566.1"
                     /translation="MRTNVEMHSARRAGGLLGSALLSLGFVLLLLAGAGAGGCARAMA
                     GDVEEARAAAAVVPGLPEWSVTFSAGRDPAADLKLALAAAKEHNRRVLVMVGGDWCVW
                     CFLLDRHFREDPE"
     gene            404..796
                     /locus_tag="PLW13_09075"
     CDS             404..796
                     /locus_tag="PLW13_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70567.1"
                     /translation="MALDIQDKFAIEELLRRACYNADYNPPESMRAIYTPDAVFEVPA
                     MDISCRGIDNIIAFFTQSRAGFSSARHVINNVVVEGDGDTAKSSAYLQVLRTDEGSVG
                     IVSVGRYLDILERSAGGWRIAHRSVAIG"
     gene            793..1794
                     /locus_tag="PLW13_09080"
     CDS             793..1794
                     /locus_tag="PLW13_09080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY70568.1"
                     /translation="MTYFVLKYDMRAPQPGAPIEALYGAALEQIAWADRNGFGTLMLA
                     EHHGNDDGYLPAPLMLAAAAAARTERMRIRVQALILPLHDPIRVAEDLAILDLISRGR
                     AEVTVAGGYLASEFRMFGVALKDRGRLVEEGIEAMKKAWSGERFVFRGREVHVTPRPL
                     QRPHPPIILGGASEAAARRAARIACGFNPAMPELHAVYRAECDRLGKAPGREERMGPV
                     FLHLAEDPDRAWARIAPHALHETNSYGRWMAESMGEKAVYRESTDAAQLRASGAYAIV
                     TPGECLQIARDLGPGGRLAFHPLMGGMDPQLGWESLELLRTRVMPHLEVEPPLLPGP"
     gene            1813..2883
                     /locus_tag="PLW13_09085"
     CDS             1813..2883
                     /locus_tag="PLW13_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY70569.1"
                     /translation="MIVFRQLFDQQSSTYTYLLADPATREAVLIDPVFEQVQREVALI
                     RELGLTLLCTLETHVHADHVTGAWLLREALGSRIVLAAAGGASGADRELRHGDVVRFG
                     GRSLEARATPGHTNGCMSYVLDDHAMVFTGDALLIRGCGRTDFQEGSARLLYRSIHEQ
                     IFTLPDECTVYPGHDYGGSTASSVGEERRFNPRLGGGIREEDFAGYMDNLGLAHPKLM
                     DKAVPANKHCGKPAVDMPAPGGQDWAPLRLTYAGLREIDPQWVEEHRGEVLVLDVREP
                     GEFDSVLGHIAGALLLPLGELGGKLDTLPRDRPVVAVCRSGARSARATQMLEQAGLRA
                     ANLAGGMLRWRAQGLAVEAGGE"
     gene            3003..4121
                     /gene="ribB"
                     /locus_tag="PLW13_09090"
     CDS             3003..4121
                     /gene="ribB"
                     /locus_tag="PLW13_09090"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQY70570.1"
                     /translation="MPISAIQDIIEDIRQGRMVILMDDEDRENEGDLIIAAEAVTPEL
                     ITFFASHACGLICLPITEERARRLALPLMVQKNQSQHETNFTVSIDAAVLPVPGITSA
                     GRAATVLAAVARDAQAADIVQPGHIFPLVAKPGGVLTRAGHTEAGCDLARLAGFEPAA
                     VIVEIVNEDGTMARRPQLEEFADRHGLRIGTIADLIAYRALHDRTIARRHEKLIETAH
                     GPLRLVSYVDQIDGALHFALIAGEIDPEQPALVRVHVINTLRDLFLAERPGKAKSWSL
                     AASLARIAAEGNGVLVLVSRRESPEDVLSQIEMFPEVPQRRGSASEMGQNFWRVNGTG
                     SQILKDLGVHRMRLLSSPTRFSAISGFNLEITEFVENV"
     gene            complement(4169..5026)
                     /locus_tag="PLW13_09095"
     CDS             complement(4169..5026)
                     /locus_tag="PLW13_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70571.1"
                     /translation="MRLQDYDHGQRFVATLLATQRITPAGGVEVRELRLELVAPDFRA
                     TAGQSVGVIVPGPHALGHAHHFRLYSLAADCEPDASGIAHVTLCVRRCNYVDEYSGEE
                     YRGVASNYLCDLAPGDRITINGPFGLPFEIPADPATDLLLISMGTGIAPFRAFVAGLY
                     RRHPDWQGKVRLFHGAMSGLELLYMNDERDDFGDYYDRATFQAFKALSPRPHWADPIA
                     MDYAIEERAAEVREMLAGESCRVYVAGKADILETLDRVFAGLAGSADAWQERKERLRA
                     ERRWFELVY"
     gene            complement(5039..5218)
                     /locus_tag="PLW13_09100"
     CDS             complement(5039..5218)
                     /locus_tag="PLW13_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70572.1"
                     /translation="MSAAAHYLQALAIVALLAGIVLGVRRLGGASAQARENIGGCGNC
                     GGCERNAPTSSAEDS"
     gene            complement(5215..5796)
                     /locus_tag="PLW13_09105"
     CDS             complement(5215..5796)
                     /locus_tag="PLW13_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HQY70573.1"
                     /translation="MPEQSLASIFLGACLINNFVLAYFLGICPFLGVSGKIATASRMG
                     AAVTFVMLVSSLCAYAIHAALAAIGALYLQLVSFIVVIAAAVQLVEMVVRKYSPALFR
                     ALGIFLPLITTNCAILALALFQTSKGYGLLQCLVYALGAGAGFTLALVLMAGIREKLE
                     LAELPDLATGTAISLFIAGILSLAFMGFAGLGA"
     gene            complement(5796..6440)
                     /locus_tag="PLW13_09110"
     CDS             complement(5796..6440)
                     /locus_tag="PLW13_09110"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HQY70574.1"
                     /translation="MSATVPGPGSPADDLLRGLWRENPVFVQVLGMCPTLAVSNSALN
                     ALTMGLATAFVLCLSSLLISLLRHVVPREVRIASFILVIATFVTVVDYLIQAISLALH
                     AALGAFISLIVVNCIILGRAEAFAARNGPLRSFLDGLGMGLGFTLALLCLGGVRELLG
                     SGSLFGIGVFGSQFEPWTVMLLPSGGFFTLGAWLLAIAWWRRRREAGARVQEAN"
     gene            complement(6437..7111)
                     /locus_tag="PLW13_09115"
     CDS             complement(6437..7111)
                     /locus_tag="PLW13_09115"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQY70575.1"
                     /translation="MSNRSAPAPTSAAAMYRALVGIGSICALLIVTAYLLTAPAIARN
                     RAELLQQAIFAVLPGAETVQPMRRDAGGTIGPAAPGEEAAFHLGLDAGNRVVGVAIEA
                     SGMGYQDLIGLIYGYSPARGQLTGMRVLASRETPGLGDKIITDPVFVGSFAGLAVPLS
                     QDGARVAHAVEAVPAGKRSQPWQFDAITGATISSRAVAGIITASTAQWAPLLHAQAGR
                     LEGDTP"
     gene            complement(7108..8127)
                     /locus_tag="PLW13_09120"
     CDS             complement(7108..8127)
                     /locus_tag="PLW13_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQY70576.1"
                     /translation="MSAPARTLDIASSPHIRAGVGVERIMFNVVLALLPVVAFAVYAF
                     GLAALMGIAVATASCVLSEHLLCRLAGKPSTVGDWSVTITGLLYGLTLPPGLALWMVA
                     LGGFLGVAMAKFLFGGLGYNAFNPALVARAFLQAAFPTAMTTWTAAFDAGRFTTLPAS
                     VLAAPFMHPAPDAMSGATPLARMKFEGELTSGVDLFLGLSAGSSGETCALLILLGGLY
                     LAARRMLDWRIPAAILGTVALLSLACHQLDPARYAPPGFMLSAGGLMLGAVFMATDMV
                     ASPVSRLGVLLYGALIGTLVVVIRYWGGMPEGVMYAILLGNAVSPHLDRLIQPVAFGA
                     RGRQR"
     gene            complement(8124..9473)
                     /gene="rsxC"
                     /locus_tag="PLW13_09125"
     CDS             complement(8124..9473)
                     /gene="rsxC"
                     /locus_tag="PLW13_09125"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQY70577.1"
                     /translation="MLPKLSRLLPRRGFAHGIHPPESKADTAAQPIRQFPFAPLMIVP
                     LLQHAGKAALPVVRAGQEVLRGELLAEPDGPLSVAMHAPAAGRIEGIELAPSISGRMQ
                     PAIYLKPHPGSTQEAIEGPPCDVDTASPEEILQAIQWAGIVGLGGAAFPTHVKLKPPP
                     GKPVDTLVINGVECEPYLSTDHRVMLEQPRDIFQGIRYLLKVSGARRALIGIEANKAD
                     AAGVLRAQRPAGLEVGVELLPVKYPQGAEKMLIKALLGRTVPAGGLPSDVGVLTVNVA
                     TCAEIGRLLPRGQGIQERVVTITGPGVRQRGNYRIPIGTPLRFALEHVGVSEELSMVF
                     LGGPMMGQALASLDIPITKGSSGFVAFTRAETGRPAVEYPCIHCGYCVQACPMNLNPC
                     DLGRFARKERFEEMADSLQLFDCFECGCCSFVCPSHIPLVQSFRVAKNAVRKKRAAP"
     gene            complement(9489..>13367)
                     /locus_tag="PLW13_09130"
     CDS             complement(9489..>13367)
                     /locus_tag="PLW13_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQY70578.1"
                     /translation="RRGVAVLERTDQPLAEDLPLMREIRATLGKCLENGKQPRDPPWP
                     ALETYRDLDELPPLYSGAFGLGSRDLQPEGLIGAIENMLPAGAQRRKYYLSIDFLHAE
                     ARTPKEALQQEAIAAAYPGIRNLALRGSENPNLMPKGSIAVRLHSIGGWGAITTGKNL
                     AMTLHELLGLHIKANPKYGSEKKGQPTTYYLAAAPEPIRINCEFYYVDVVLSPDPHVL
                     AHTNALAGLRDGGVFVLQSDLGSPDEVWASIAQHYRQQIVDRDIRVFYLDAFAIARAE
                     ATDPELQLRMQGIAFQGAFFATSPLMQEAGLTGDKLLGAIRAQLQHKFGAKGARVVED
                     NIRVVRRGFDEVREITNRSVGEKAPAARQGAGELPVPVRELPQSRAAQSDIHRFWEQT
                     GNFYARGQGSDNIADPFISLGVMPASSAMFRDMSGIRFQHPRWIAENCTACGKCYTVC
                     PDTAIPGLVSEIGAVFDTVVTRLRRRGIAAQQLPGHLRRLEPRLRQLLNAAADEDAVP
                     QMLRMAVGDYLSAQIDAGQGSTLRAEFDAFLEEIGSFQFALSKPYFRVPEKGEAGSGG
                     LLSITVNPYTCKGCMECVAVCDDNALEALVQTPASVEELRRNWDFWRDLPTTPPRYIR
                     VEDLEQGIGALETILLDKQAYLSFASGDGACLGCSEKTVVHLFVATVEALMRPRVERH
                     VAYLDGLIGRLERHMQTRLIAEVNVDDTEAIAAALAAEDGGELTLAGLARRIEAQQGS
                     QPIDSAWLKRMNSLVAGLKELRWRYREGTTGRGRSNMGMLNSTGCSSVWGSTWPFNPY
                     PFPWANHLFQDSPSLAMGVFEGHMAKMAAGFRLVRSAELELEGRYVAAEHEEFLARFD
                     WQQFSDEEFALCPPVVALGGDGAMYDIGFQNLSRAMMSGKPIKVVVLDTQVYSNTGGQ
                     ACTSGFLGQVSDMAQFGAAQSGKQEIRKEIGLIGLAHRTSYVMQSTIAHPGHMIEGFI
                     QGLQARRPALFNLYTSCQPEHGIGDDMGHEQARLAVESRAYPLFRYDPARGRTLAECI
                     DLSGNPAQAETWPSYRLDYREGGRAQSMQLPLTFADFAMTEARFRKHFRTAPPDTWHE
                     NMVPLAEFLALEADEREGKYPFIWTVDRRQQLGRLLVDATMVRSTEERRDFWFLLRDL
                     ARTASATAPAARGEAEIERAVMARLTAGLLRLASDPGSVAALEPAGVPGADLATATTP
                     AAGGGEAMAPWLDSAECTSCDECTRINDAIFAYGADGKAYIKDPAGGPYGDLVRAAEK
                     CTARVIHPGLPADRSAPDIAKWIERGEKYN"
BASE COUNT         2067 a         4707 c         4681 g         1912 t
ORIGIN      
        1 ttcaggatcc tcgcggaaat ggcggtccag caggaagcac cacacgcacc agtccccgcc
       61 caccatcacc agcacgcggc ggttgtgctc cttcgccgcg gcgagcgcga gcttcaggtc
      121 ggcggccgga tcacgcccgg cagagaaggt gacggaccac tccggcagcc ccggcaccac
      181 cgccgcggcg gcgcgcgcct cctcgacatc gcccgccatg gcgcgcgcgc acccgccggc
      241 gccggcgccg gcgagcagca ggagcacgaa gcccagtgac agcagagcgc tgccgagcag
      301 gccgccagcg cgcctggcgg aatgcatctc gacgtttgtt cgcatgggaa ttaccctaga
      361 atctgggccc cgccaccgtg gcggaacaca ccggaggttg atgatggcac tggacatcca
      421 ggacaagttc gcgatcgagg agctgctgcg gcgcgcgtgt tacaacgccg actacaaccc
      481 gcccgaatcg atgcgcgcga tatacacgcc cgatgccgtg ttcgaggtgc cggcgatgga
      541 catcagctgc aggggcatcg acaacatcat cgccttcttc acccagagcc gcgcgggctt
      601 ctcctcggcg cggcatgtta tcaacaacgt cgtggtcgag ggagacggcg acacggcgaa
      661 atcctccgcc tacctgcagg tgctgcgcac cgacgagggc tcggtcggca tcgtgtcggt
      721 ggggcgctac ctcgacatcc tggagcgcag cgccggcggc tggcgcatcg cgcaccgctc
      781 ggtggcgatc ggatgaccta tttcgtcctg aagtacgaca tgcgcgcgcc gcagcccggc
      841 gcgcccatcg aggcgctcta cggcgccgcc ctcgagcaga tcgcctgggc cgaccgcaac
      901 ggcttcggca ccctgatgct ggccgagcac cacggcaacg acgacggcta cctgccggcg
      961 ccgctcatgc tggcggccgc cgcggccgcg cgcacggagc gcatgcgcat ccgcgtgcag
     1021 gcgctgatcc tgcccctgca cgacccgatc cgcgtcgccg aggacctcgc gatcctcgat
     1081 ctcatcagcc gcggccgtgc cgaggtgacg gtggccggcg gctacctcgc ctcggagttc
     1141 cgaatgttcg gcgtcgcgct gaaggatcgc gggcgccttg tcgaggaagg catcgaggcg
     1201 atgaagaagg cctggagcgg cgagcgtttc gtgtttcgcg gccgtgaggt gcacgtcacg
     1261 ccgcgcccgc tgcagcgccc gcacccgccg atcatcctcg gcggtgcgag cgaggccgcc
     1321 gcgcggcgcg ccgcgcgcat cgcctgcggc ttcaaccccg cgatgcccga actgcacgcg
     1381 gtgtatcgcg ccgagtgcga tcgcctcggc aaggccccgg ggcgcgagga gcgcatgggg
     1441 ccggtgttcc tgcacctcgc cgaggatccc gaccgcgcct gggcgcgcat cgcgccgcac
     1501 gcgctgcacg agaccaacag ctacgggcgc tggatggcgg aatcgatggg cgagaaagcc
     1561 gtgtaccgtg aatccacgga tgcggcgcaa ctgcgcgcta gcggcgccta cgcgatcgtc
     1621 acgcccgggg agtgcctgca gatcgcgcgc gacctcggcc cgggcgggcg cctcgccttc
     1681 catccgctga tgggcggcat ggacccgcag ctcggctggg aatcgctcga gctgctgcgc
     1741 acccgcgtga tgccgcacct cgaggtcgag cccccgctgc tgcccggccc ctgaacaact
     1801 ggagaagaca ccatgatcgt gttccggcaa cttttcgacc aacagtcctc cacctacacc
     1861 tacctgctcg cggaccccgc gacccgcgag gcggtgctga tcgacccggt gttcgagcag
     1921 gtgcagcgcg aggtcgcgct gatccgcgag ctcgggctga cgctgctgtg cacgctcgag
     1981 acccatgtgc acgccgatca cgtgaccggc gcctggctgc tgcgcgaggc actgggctcg
     2041 cgcatcgtgc tggccgcggc aggcggcgcg agcggggccg atcgcgaact gcgccacggc
     2101 gacgtggtgc gcttcggcgg gcgcagcctc gaggcgcgcg ccacgccggg gcacaccaac
     2161 ggctgcatga gctacgtgct cgacgaccat gccatggtgt tcacgggcga tgcgctgctg
     2221 atccgcggct gcgggcgcac cgatttccag gaaggcagcg ctcgcctgct ctaccgctcg
     2281 atccacgagc agatcttcac gcttcccgat gagtgcacgg tctaccccgg gcacgactac
     2341 ggcggttcca cggcctcgtc ggtcggcgag gaacgacgct tcaacccgcg cctcggcggg
     2401 gggatccgcg aggaggattt cgccggctac atggacaacc tgggcctcgc gcaccccaag
     2461 ctgatggaca aggccgtgcc ggccaacaag cactgcggca agccggccgt ggacatgccc
     2521 gcgccgggcg ggcaggactg ggcaccgttg cgcctcacct acgcggggtt gcgcgagatc
     2581 gatccgcaat gggtagaaga gcaccgcggc gaggtgctgg tgctcgacgt gcgcgagccg
     2641 ggcgagttcg acagcgtgct cgggcacatc gccggcgcgt tgctgctgcc gctcggggag
     2701 ctgggcggga aactcgacac cctgccacgc gatcgccccg tggtcgcggt gtgccgctcg
     2761 ggggcgcgtt cggcgcgcgc cacgcagatg ctcgagcagg cgggtttgcg cgccgccaac
     2821 ctcgccggcg gcatgctgcg ctggcgcgcg caggggctgg cggtcgaggc cggcggggaa
     2881 tgacgcgcca ccgcgcgtga tgcgccggag cgcgccggcc gggcacggac gcccggctgg
     2941 tgcccgtcgg ccattgtccc taagataccg ccctcgccaa gcccagcacc ccggaacccg
     3001 ccatgcccat cagcgccatc caggacatca tcgaggacat ccgccagggt cgcatggtga
     3061 tcctgatgga cgacgaggac cgcgagaacg agggcgatct gatcatcgcc gccgaggcgg
     3121 tgacgccgga gctgatcacc tttttcgcct cgcatgcctg cggcctgatc tgcctgccga
     3181 tcaccgagga gcgcgcgcgc cgcctggcgc tgccgctgat ggtgcagaag aaccagtccc
     3241 agcacgagac caacttcacc gtctcgatcg acgccgccgt gctgccggtg cccggcatca
     3301 cctccgcggg acgcgccgcc accgtgctcg cggcggtcgc gcgcgacgcg caggccgccg
     3361 acatcgtgca gccgggccac atcttccccc tggtcgccaa gcccggcggc gtgctgacgc
     3421 gcgccggcca caccgaggcg ggttgcgacc tcgcgcggct cgcgggcttc gagccggcgg
     3481 cggtgatcgt ggagatcgtg aacgaggacg gcaccatggc gcgccgcccg cagctcgagg
     3541 agttcgcgga tcgtcacggg ctgcgcatcg gcaccatcgc cgacctcatc gcctaccgcg
     3601 cgctgcacga ccggaccatc gcgcgccgcc acgagaagct gatcgagacc gcgcacgggc
     3661 cgttgcgcct ggtgagctac gtcgaccaga tcgacggcgc gctgcatttc gcgctgatag
     3721 ccggcgagat cgatcccgag cagccggcgc tggtgcgcgt gcacgtgatc aacacgctgc
     3781 gcgacctgtt cctggccgag cgtcccggca aggcgaagag ctggtcgcta gccgcttcgc
     3841 tggcgcgtat cgcggccgag ggcaacggcg tgctggtgct cgtgtcgcgg cgcgagagcc
     3901 ccgaggacgt gctcagccag atcgagatgt tcccggaagt accgcagcgg cgcggcagcg
     3961 ccagcgagat ggggcagaac ttctggcgcg tgaacggcac cggctcgcag atcctgaagg
     4021 acctcggcgt gcaccgcatg cgcctgctga gctcgcccac gcgcttcagc gcgatctcgg
     4081 gcttcaacct cgagatcacg gagttcgtcg agaacgtgta actccgcatt aatgcggacg
     4141 ggtgcatgac gcgcgccgac acccgccctc agtacaccag ctcgaaccag cgccgctcgg
     4201 cgcgcagccg ctccttgcgc tcctgccagg catcggccga gccggccagg cccgcgaaca
     4261 cgcggtccag cgtctcgagg atgtcggcct tgccggcgac gtacacccgg cagctctcgc
     4321 ccgcgagcat ttcccggacc tcggccgcgc gctcctcgat cgcgtagtcc atcgcgatgg
     4381 ggtcggccca gtgcgggcgc ggactcagtg ccttgaaggc ctggaaggtc gcgcggtcgt
     4441 agtagtcgcc gaagtcgtcg cgctcgtcgt tcatgtacag cagttcgagc ccgctcatcg
     4501 cgccgtggaa caggcggacc ttgccctgcc agtccgggtg gcggcggtag agcccggcga
     4561 cgaaggcgcg aaacggcgcg atgccggtgc ccatgctgat cagcagcagg tccgtggccg
     4621 ggtccgcggg aatctcgaac ggcaggccga acggtccgtt gatcgtgatc ctgtcccccg
     4681 gcgccaggtc gcacaggtaa ttggaagcca cgccgcggta ttcctcgccg ctgtattcat
     4741 cgacatagtt gcagcgcctc acgcacagcg tgacgtgcgc gatgccggag gcatcgggct
     4801 cgcagtcggc ggcgaggctg tagaggcgga aatggtgcgc atgaccgagc gcgtgcggcc
     4861 ccggcacgat cacgccgacg ctctggccgg ccgtggcgcg aaaatccggc gccaccagtt
     4921 ccagccgcag ttcgcgcacc tcgacgccgc ccgcgggcgt gatgcgctgg gtggcgagca
     4981 gcgtggcgac gaaacgctgg ccgtgatcgt agtcctgcag tcgcatggtg cctcccgttc
     5041 aggaatcttc cgctgatgat gtcggtgcat tgcgttcgca gccgccgcaa ttgccgcagc
     5101 cgccgatgtt ctcccgcgct tgcgcgctgg cgccgccgag gcgacgcacg ccgagcacga
     5161 tgccggcgag cagcgccacg atcgccagcg cctgcaggta atgggccgcg gcgctcatgc
     5221 gcccagcccc gcgaaaccca tgaacgcgag cgagaggatg cccgcgatga acaggctgat
     5281 cgcggtcccg gtggccaggt cgggcagctc ggcgagctcg agcttttcgc ggatgccggc
     5341 catcagcacc agcgccagcg tgaagccggc accggcgccg agggcataga ccaggcattg
     5401 cagcagcccg tagcccttgc tggtctggaa cagggcgagc gcgaggatcg cgcagttcgt
     5461 ggtgatcagc ggcaggaaga tcccgagcgc gcggaacagc gccgggctgt acttgcggac
     5521 caccatctcg acgagttgca ccgcggcggc gatcaccacg atgaacgaca ccagctgcag
     5581 gtagagcgcc ccgatcgccg ccagcgccgc gtggatcgcg taggcacaca gcgagctgac
     5641 cagcatcacg aaggtcacgg ccgcgcccat gcggctcgcg gtggcaatct tccccgatac
     5701 gcccaggaag gggcagatgc ctagaaagta cgccagcacg aagttgttga tcaggcaggc
     5761 gccgaggaat atcgaggcca gtgactgttc aggcatcagt tcgcctcctg cacgcgcgcg
     5821 ccggcttcgc gccggcgccg ccaccaggct atcgccagca gccaggcgcc aagggtgaag
     5881 aaaccgccgc tcggcagcaa catcacggtc cacggttcga actggctgcc gaagacaccg
     5941 atcccgaaca ggctgccgct gcccagcagc tcgcgcaccc cgcccaggca gagcagcgcg
     6001 agcgtgaatc ccaggcccat gccgaggccg tcgaggaagg agcgcagcgg gccattgcgc
     6061 gcggcaaagg cctcggcgcg cccgaggatg atgcaattga ccacgatcag cgaaatgaag
     6121 gcaccgagcg cggcgtgcag cgcgaggctg atcgcctgga tcaggtagtc gacgacggtg
     6181 acgaaggtgg cgatgacgag gatgaagctg gcgatacgca cttcgcgcgg caccacgtgg
     6241 cgcagcaacg agatcagcag gctcgacagg cacagcacga atgcggtcgc caggcccatg
     6301 gtcagggcgt tgagcgccga attgctcacg gcgagcgtcg ggcacatgcc gaggacctgc
     6361 acgaacaccg ggttctcgcg ccacagcccc cgcaggagat catcggccgg gctgccgggc
     6421 cctggcactg tcgcgctcac ggggtgtccc cttcgagccg cccggcctgc gcgtgcagca
     6481 gcggcgccca ctgcgcggtg ctggccgtga tgatgccggc gacggcgcgc gaggaaatcg
     6541 tcgcgccggt gatcgcgtcg aactgccacg gctggctgcg cttgccggca ggcaccgcct
     6601 ccacagcgtg tgccacgcga gcgccgtcct gcgacagcgg taccgcgagg ccggcgaagg
     6661 agcccacgaa gacgggatcg gtgatgatct tgtcgcccag tcccggcgtc tcgcggctgg
     6721 cgagcacgcg catcccggtc agctggcccc gcgccggcga atagccgtag atcaggccga
     6781 tcaggtcctg gtagcccatg ccgctggcct cgatcgcgac accgaccacg cgattcccgg
     6841 cgtcgagccc gaggtggaac gcggcctcct ctcctggtgc ggccggccct atcgtgcccc
     6901 cggcgtcgcg gcgcatgggc tgcaccgtct ccgccccggg cagcacggcg aagatcgcct
     6961 gctgcagcag ctcggcgcga ttgcgcgcga tcgccggagc ggtcagcagg taggccgtca
     7021 cgatcagcag cgcgcagatg ctgccgatgc cgaccagcgc gcggtacatc gccgcggcac
     7081 tggtgggcgc gggtgcggat cggttgctca tcgctgccgg cccctggcgc cgaaggccac
     7141 cggctggatc aacctgtcga gatgcggcga caccgcgttg ccgagcagga tcgcgtacat
     7201 cacgccttcg ggcatgccgc cccagtagcg gatcaccacg accagcgtgc cgatcagcgc
     7261 gccgtagagc agcacgccga ggcggctcac cggcgaggcg accatgtcgg tggccatgaa
     7321 taccgcgccc agcatcaggc cgccggccga gagcatgaaa ccgggcggcg cgtagcgcgc
     7381 cgggtcgagc tggtggcagg ccaggctcag cagcgcgacg gtgccgagta tcgccgccgg
     7441 gatgcgccag tcgagcatgc gccgcgccgc caggtagagg ccgccgagaa ggatcagcag
     7501 cgcgcaggtc tcgccgctcg atcccgcgct cagcccgagg aagagatcga cgcccgaggt
     7561 cagctcaccc tcgaacttca tccgcgccag cggtgtcgcg ccgctcatcg catcgggtgc
     7621 cgggtgcatg aacggggcgg ccagcaccga ggcgggcaac gtggtgaagc gcccggcatc
     7681 gaaagcggcc gtccaggtcg tcatggccgt cgggaacgcc gcctgcagga atgcccgcgc
     7741 gaccagcgcc gggttgaagg cgttgtagcc gagtccgccg aacaggaact tggccatcgc
     7801 cacgccgagg aaaccgccga gcgccaccat ccacagcgcc agccccggtg gcagcgtcag
     7861 cccgtagagc aggccggtga tggtgaccga ccagtcgccc accgtgctcg gcttgccggc
     7921 caggcggcac agcaggtgct cgctcagcac gcaggaggcg gtcgccaccg cgatgcccat
     7981 tagcgcggcg aggccgaagg cgtagacggc gaaggcaacc accggcagca gcgccagcac
     8041 cacgttgaac atgatgcgct cgacaccgac cccggcgcgg atgtgcggcg aggaggcgat
     8101 atcgagggtt cgcgccggcg cgctcacggc gcggccctct tcttgcgcac ggcgttcttg
     8161 gcgacgcgga acgactgcac cagcggaatg tgcgacggac acacgaagga acagcagccg
     8221 cattcgaagc agtcgaacag ctgcagggag tcggccatct cctcgaagcg ctccttgcgc
     8281 gcgaagcgcc cgagatcgca gggattgaga ttcatcgggc aggcctgcac gcagtagccg
     8341 cagtggatgc acgggtactc gaccgcgggc cgacccgttt cggcgcgcgt gaaggcaacg
     8401 aatcccgagc tgcccttggt gatcggtatg tcgagactcg cgagagcctg gcccatcatc
     8461 ggcccgccga ggaagaccat gctgagctcc tcgctgacgc cgacgtgctc gagggcgaag
     8521 cgcagcggcg tgccgatcgg gatgcggtag ttgccgcgct ggcgcacgcc cggcccggtg
     8581 atcgtcacca cgcgctcctg gatcccctgc ccgcgcggca gcaggcggcc gatctcggcg
     8641 caggtggcga cgttgaccgt gagcacgccg acatcgctgg gcagcccgcc ggcgggcacc
     8701 gtgcgcccga gcagcgcctt gatcagcatt ttctcggcgc cctgcggata cttgaccggc
     8761 agcagctcca cgccgacctc gagccccgcg gggcgctgcg cgcgcagcac cccggcggca
     8821 tcggccttgt tggcctcgat gccgatcagc gcgcgccgcg cgccgctgac cttcagcagg
     8881 tagcggatgc cctggaagat gtcgcgtggc tgctccagca tgacccggtg gtcggtgctc
     8941 aggtagggct cgcattccac gccgttgatc accagcgtgt cgacgggctt gccgggcggg
     9001 ggcttcagtt tgacgtgggt cggaaacgcc gcgccgccca gcccgacgat gccggcccac
     9061 tggatcgcct gcaggatctc ctcggggctc gccgtgtcca catcgcaggg cggtccctcg
     9121 atcgcctcct gggtcgagcc ggggtgcggt ttgagataga tcgccggctg catgcgccca
     9181 ctgatcgacg gcgccagctc aatgccctcg atacgaccgg cggccggcgc gtgcatggcg
     9241 accgacagcg ggccgtcggg ctcggccagc agttcgccgc gcaacacctc ctggcccgcg
     9301 cgcaccaccg gcagtgccgc tttgccggca tgctgcagca gcggcacgat catcagcggc
     9361 gcgaacggga actgccggat cggttgcgcg gccgtgtcgg ccttcgactc gggcgggtgg
     9421 atgccgtggg cgaagccgcg ccgcggcagc aggcggctca gcttcggcaa catggttcgc
     9481 tgctacgctc agttgtactt ctcaccgcgc tctatccatt tggcgatatc gggcgcactg
     9541 cggtcggcgg gcagcccggg atggatgacc cgcgcggtgc acttctccgc ggccctgacc
     9601 aggtcgccgt agggcccgcc ggccgggtcc ttgatgtacg ccttgccgtc ggcgccgtag
     9661 gcgaagatcg cgtcgttgat gcgcgtgcac tcgtcgcagg aggtgcactc ggccgaatcc
     9721 agccacggcg ccatggcctc gccgccgccc gccgccggcg tcgttgccgt ggcgaggtcc
     9781 gcgccgggca cgccggccgg ctcgagcgcg gccacgctgc ccggatcgct cgccaggcgc
     9841 agcaggccgg cggtgaggcg cgccatgacc gcgcgctcga tctcggcctc gccgcgcgcg
     9901 gcgggcgccg tcgcgctagc cgtgcgcgcc aggtcgcgca gcaggaacca gaaatcgcga
     9961 cgctcctcgg tgctgcgcac catcgtggcg tcgaccagca ggcgaccgag ctgctggcgc
    10021 cggtccaccg tccagatgaa cggatacttg ccctcgcgct cgtccgcctc gagcgcgagg
    10081 aactcggcca gcgggaccat gttctcgtgc caggtgtcgg gcggcgcggt acggaaatgc
    10141 ttgcgaaagc gcgcctcggt catcgcgaaa tcggcgaagg tcagcggcag ctgcatcgac
    10201 tgcgcgcgcc cgccctcgcg gtaatccagc cggtagctcg gccaggtttc ggcctgcgcc
    10261 gggtttcccg acaggtcgat gcactcggcg agcgtgcgcc cgcgcgcggg gtcgtagcga
    10321 aacagcgggt aggcgcgcga ctcgaccgcc aggcgcgcct gctcgtgccc catgtcatcg
    10381 ccgatgccgt gctcgggctg gcaggaggtg tagaggttga acagggcggg gcggcgcgct
    10441 tgcaggccct ggatgaagcc ctcgatcatg tggccggggt gcgcgatcgt gctctgcatc
    10501 acgtagctgg tgcgatgcgc caggccgatc aggccgatct ccttgcggat ctcctgcttg
    10561 cccgactgcg cggcaccgaa ctgcgccatg tccgagacct gcccgaggaa tccggaggtg
    10621 caggcctggc cgccggtatt cgaatacacc tgcgtgtcga gaaccaccac cttgatcggc
    10681 ttgcccgaca tcatcgcccg cgacaggttc tggaaaccga tgtcgtacat cgccccgtcg
    10741 ccgcccagcg cgaccaccgg cggacacagc gcgaactcct cgtcgctgaa ctgctgccag
    10801 tcgaagcggg cgaggaactc ctcgtgctcg gcggcgacgt agcggccctc gagctcgagc
    10861 tcggcgctgc gcaccaggcg aaagcccgcg gccatcttgg ccatgtgccc ttcgaacacg
    10921 cccatcgcga gcgaggggga atcctggaac aggtggttgg cccacgggaa cgggtaggga
    10981 ttgaacggcc aggtgctgcc ccagaccgag gagcagcccg tggagttcag catgcccatg
    11041 ttcgagcgcc cgcgcccggt cgtgccctcg cggtagcgcc agcgcaactc cttcagcccg
    11101 gccaccaggg aattcatccg cttcagccag gccgagtcga tgggctgcga gccctgctgc
    11161 gcctcgatcc gccgcgcgag cccggccagc gtgagctcgc cgccgtcctc ggccgcgagt
    11221 gccgcggcga tggcctcggt gtcgtcgacg ttaacctcgg cgatcaggcg cgtctgcatg
    11281 tggcgctcca ggcgcccgat cagcccgtcc aggtaggcga cgtggcgctc cacgcgaggt
    11341 cgcatcagcg cctccaccgt cgcgacgaac aggtgcacca cggtcttttc cgagcagccg
    11401 aggcaggcgc cgtcgccgct ggcgaaagac aggtaggcct gcttgtcgag caggatggtc
    11461 tccagcgcgc cgatgccctg ctcgaggtcc tcgacgcgga tgtagcgcgg cggcgtggtc
    11521 ggcaggtcgc gccagaaatc ccagttgcgc cgcagctcct cgacgctcgc cggcgtctgc
    11581 accagcgcct cgagcgcgtt gtcgtcgcag accgcgacgc actccatgca gcccttgcag
    11641 gtgtaggggt tgaccgtgat gctgagcagg ccgccgctgc cggcctcgcc cttttccggg
    11701 acacggaagt agggtttgct cagcgcgaac tggaagctgc cgatctcctc gaggaaggca
    11761 tcgaattccg cgcgcagcgt cgagccctgc ccggcatcga tctgggccga caggtagtcg
    11821 ccgacggcca tgcgcagcat ttgcggcacc gcgtcctcgt ccgccgcggc gttgagcagc
    11881 tggcgcagcc gcggctcgag ccggcgcagg tgcccgggga gctgttgcgc cgcgatgccg
    11941 cgccggcgca ggcgcgtcac cacggtgtcg aataccgcgc cgatctcgct gaccaggccg
    12001 ggaatcgcgg tgtccgggca cacggtgtag cacttgccgc aggcggtgca gttctccgcg
    12061 atccagcgcg ggtgctggaa gcggatgccg ctcatgtcgc ggaacatcgc cgagctggcg
    12121 ggcatgacgc cgaggctgat gaaggggtcg gcgatgttgt ccgagccctg gccgcgggcg
    12181 tagaagttgc cggtctgctc ccagaaacgg tggatgtcgc tctgcgccgc gcggctctgc
    12241 ggcagctcgc gcaccggcac cggcagctcg ccggcgccct ggcgcgcggc gggcgccttc
    12301 tcgcccacgc tgcggttggt gatctcgcgc acctcgtcga agccgcggcg caccacgcgg
    12361 atgttgtcct cgaccacgcg cgcgcccttg gcgccgaact tgtgctgcag ctgcgcgcga
    12421 atcgcgccga gcagcttgtc cccggtcagc cccgcctcct gcatcagcgg cgaggtggcg
    12481 aagaaggcgc cctggaacgc gatgccctgc atgcgcagct gcagctccgg atcggtggcc
    12541 tcggcgcgcg cgatcgcgaa ggcgtcgagg tagaagacgc ggatgtcgcg atcgacgatc
    12601 tgctggcggt agtgctgcgc aatgctggcc cacacctcgt cgggcgagcc gagatcgctc
    12661 tgcagcacga agaccccgcc gtcgcgcagt cccgccagcg cgttggtgtg cgcgagcacg
    12721 tggggatcgg gcgacagcac cacgtcgacg tagtagaact cgcagttgat gcggatcggt
    12781 tccggcgcgg cggcgaggta gtaggtcgtc ggctggccct tcttctccga accgtacttg
    12841 gggttggcct tgatgtgcag gccgagcagc tcgtggagcg tcatcgcgag gttcttgccc
    12901 gtcgtgatcg ccccccagcc cccgatcgag tgcaggcgca ccgcgatgct gcccttgggc
    12961 atcaggttcg ggttctcgct gccgcgcagc gccaggttgc ggatcccggg gtaggccgcg
    13021 gcaatcgcct cctgctgcaa cgcctccttg ggcgtgcgcg cctcggcgtg caggaaatcg
    13081 atcgacaggt agtacttccg gcgctgcgcg cccgcgggca gcatgttctc gatcgcgccg
    13141 atcagcccct cgggttgcag gtcgcggctg ccaaggccga aggcgccgga gtacagcggt
    13201 ggcagctcgt cgagatcgcg gtaggtctcc agcgccggcc acggcgggtc gcgcggctgc
    13261 ttgccgttct ccaggcactt gcccagcgtc gcgcggatct cgcgcatcag cggcaggtcc
    13321 tcggccagcg gctggtcggt gcgctcgagc accgcgacgc cccggcg
//
LOCUS       DAORUQ010000088        13162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4661_length_13162_cov_5.871977, whole genome shotgun sequence.
ACCESSION   DAORUQ010000088 DAORUQ010000000
VERSION     DAORUQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_13162_cov_5.871977"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..1859)
                     /locus_tag="PLW13_09135"
     CDS             complement(348..1859)
                     /locus_tag="PLW13_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY70579.1"
                     /translation="MQHHHTAPVSSWLRFRDVSVRLHGKLFLRGLDWSAAPGEQWAVL
                     GPNGAGKTVLAQLIARALHPASGELTLAADLTPARIAWVSFEAQRELCAADARHDISD
                     YLENAVDAGTTVRALLAAAARRPERVEEIAAMLGIEALMERGIRFLSSGETRRTLFAR
                     ALLMEPRLMVLDNPYEGVDRASRQVLRALVDELLGGPTHILLLTRRPQDIAPGISHVL
                     LLDHGQVLDRGPREAVLEGDASQRLFGPGASCAPGPLPAAPDAAPARPCAGALIELED
                     VHAHYGEHEVLDGVSLRLAPGEHLCIAGPNGCGKSTLLALICGDNPRAYGQHVKLFGR
                     LRGSGESVWEIKRRFGIVSNQLHLSYPARTRAFDVVASGFFDTLGLYDTCGPQQARLA
                     REWLAVLGLSARAGERFDALSFGEQRMMLVARAMVKNPPILILDEPCSGLDEANRRRV
                     LELIERIGHETNTQILYVSHEAEEMPACITRRLEFRRDAAGRYRLEPTELRPA"
     gene            1969..2562
                     /locus_tag="PLW13_09140"
     CDS             1969..2562
                     /locus_tag="PLW13_09140"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQY70580.1"
                     /translation="MSPALRDIGAAVQLLAEHRPRRKLVRRILARSAVGLYLSDHLPG
                     ALSVLMIKRAEREGDPWSGHMAFPGGRKDRGDRNTLATAKRETLEEVALDADRHARLL
                     GRLSDIATQPGLRGAPMIITPYVFQLHSLPPLVPNHEVAGLVWVPLPFLADRANREKM
                     RLAPNGVPIELPCYMFGEHRIWGLSLMMLDELLDALA"
     gene            2731..5049
                     /locus_tag="PLW13_09145"
     CDS             2731..5049
                     /locus_tag="PLW13_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQY70581.1"
                     /translation="MTERWLTLCERLLLARPWHSLLVILALLLIVGAHIPDLRFDAAP
                     DTLLADDPASGFAAEIAAEFGPQEFLLVTYRPQRDDLMAERVLERIARLRAALAAVAG
                     VHDVSSILEVPLLFSPPIRFADIGGELQRLQHTGVDTELARAEFRQSPLYRQSLLSGD
                     GQETALRVTLEPGAAEALLQGQQRLLDDLQAVLERHRDEATLFLGGPTLTAAAMPAFV
                     QRDLVRFGAATLAVMALVLVALLRRLPWALVPPAAATACGVLLLGLLGWSGRPLGGVS
                     AYALPVALVAAATMALRLVCAYGECLARQPARAPREVLGETLRAALAPLLGGAAVAAV
                     AAAALASSPLVAVAALGVTAASGIALAALVACVLVPVLLLLGAGGGRQPVAPARAGVL
                     LGGLAGRYGISVALLALALLAGGLAGMQKLQPEDRLLERFGPSSELFQGMLNIEREFG
                     GAETLAIVLESDTPQQAGAGSANPWYSARGLQRLRQAHDYLDALPESAAVRSLALFGA
                     TATELLDGPADDKQLAAAWRGMPAARRALLLDPWLSASTARTLIVVGLNASGPAPARH
                     ELIERIRAQLVGELGFAPGQVRIGGLAVLEGNALRTLWRTQFIAVAAACAALALLVLA
                     RWRSPLLALLALAPSLCTAVVVGGAMGWFGVPLGTLSVAFAALAAASCAAATLHCLWR
                     VGADRASAPQRCGAGTAALLCYIPLGVGAPFALLAASDFRPSAHFGLLCPLALAAASG
                     FAALLPPLLRWSRLPAGRRPRAGDDDFHSADA"
     gene            5057..5659
                     /locus_tag="PLW13_09150"
     CDS             5057..5659
                     /locus_tag="PLW13_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQY70582.1"
                     /translation="MNTEELPLFPLSAVLLPGGRVPLQIFEPRYLDLVSRCMKTDSGF
                     GIVRITSGHEVIIAQGTTPPQTAPIGTLARIVDWDALPAGRLRITVEGSRRFRVATTR
                     VQADGLLWGEVQWLAEPGNGPVADEHQFLQDIMSGLAEHPALARLGVRGSWADRASLS
                     WALAQYLPLEEAERYAVLAEPEPLARLAYLERLVKAMGSS"
     gene            complement(5637..6263)
                     /locus_tag="PLW13_09155"
     CDS             complement(5637..6263)
                     /locus_tag="PLW13_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HQY70583.1"
                     /translation="MKLEFFFDCSSPWTYLAFEGVQPIAERHGVAIDWRPILVGGVFN
                     AVNQEVYAARANPNPRRWQYTMKDLADWARYYGLEIGWPAVFPVNSVKAMRGALVALD
                     EGRLVPYARAVFQRYWRDLADISQPQVVADIAAAVGFDGPAFLERIEQPALKERLRRN
                     TEELVARGGFGTPTLFLDGEDMYFGNDRLVLVERAIVTQRGLRNSPSP"
     gene            complement(6276..6926)
                     /locus_tag="PLW13_09160"
     CDS             complement(6276..6926)
                     /locus_tag="PLW13_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQY70584.1"
                     /translation="MPFASFDASALQPWLEQHPAWVAAAIALISFAESFAILGIAVPG
                     VALLGVAAFVAGSGALGLPECLLAAFAGAVAGDGVSFLLGRIFHDDIKRAWPFRRNPE
                     WIANGERFFARHGAKSVAIGRFVGPIRPVIPLVAGILNMRARPFFLINAVSALAWAPV
                     YILPGYYLGAAVETHLASPRILLTMVVVALAAAGLVFWIHRRFLAGNGGGPVPPHD"
     gene            7075..8331
                     /locus_tag="PLW13_09165"
     CDS             7075..8331
                     /locus_tag="PLW13_09165"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQY70585.1"
                     /translation="MDIGSYMADVGREARAASRLMARATTQAKNNALQEAGAAIAAAR
                     AELVAANGDDMTRAAAQGLDAAALDRLKLTDARIDAMIEGLEQVATLPDPVGAITDLA
                     YRPSGIQVGRMRVPLGVVGIIYESRPNVTADAASLCLKSGNAAILRGGSESLASNRVI
                     ASCMHRGLRAAGLPERALQVIATADRGAVGLLITMPEYVDVIVPRGGKGLIERISAEA
                     RVPVIKHLDGICHVYIDEAADLDKAFAVALNAKTQRYGTCNTMETLLVAAPVAAAILP
                     RLATAYTEHGVELRGCARTRELLPRALAATEEDWGTEYLAPILAIRVVAGIDEAMEHI
                     ARYGSQHTDAIVTEDYSRARRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKI
                     HARGPVGLEGLTSQKYVVLGDGHVRR"
     gene            8350..9033
                     /gene="nadD"
                     /locus_tag="PLW13_09170"
     CDS             8350..9033
                     /gene="nadD"
                     /locus_tag="PLW13_09170"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQY70586.1"
                     /translation="MNDSAARRIGILGGTFDPVHNGHLRTALELAELLALDELRLVPS
                     SVPPHRGAPLATAAERLRMLQLATDAHPGFVVDDRELVRGGASYTIDTLRSLRAELGA
                     AARLCLVVGLDAFAAIDTWREWRALGEHAHIVVVQRPGVAPALHEAVARWASARLAAD
                     AGTALCAAAHGAVLRLELTQLAISATHIRELLAQGRSPRYLLPEAVLDYIREHRIYTR
                     APAHTGDTD"
     gene            9030..9374
                     /gene="rsfS"
                     /locus_tag="PLW13_09175"
     CDS             9030..9374
                     /gene="rsfS"
                     /locus_tag="PLW13_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQY70587.1"
                     /translation="MSKKDSAELAALAVRAIEDLKGQDIVRLDVRKLTDVTDYLVIAT
                     GTSSRHVKSIANNVLEELRNAGERPIGVEGTEAGEWILADFGGVVVHVMGAEPRRFYA
                     IEKLWSEMPETR"
     gene            9374..9835
                     /gene="rlmH"
                     /locus_tag="PLW13_09180"
     CDS             9374..9835
                     /gene="rlmH"
                     /locus_tag="PLW13_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQY70588.1"
                     /translation="MAARLRILAVGTRMPAWVQAGFAEYHKRIPRDFAVELREIPVSR
                     REGARAVAEEGEALLAALGADEHVVALEVGGEPWSTGDLASRLRVWRDEGRRAAFLIG
                     GPDGLADACRARAASQWSLSRLTLPHPLVRIVLMEQIYRAWSILGAHPYHR"
     gene            9843..11858
                     /gene="mrdA"
                     /locus_tag="PLW13_09185"
     CDS             9843..11858
                     /gene="mrdA"
                     /locus_tag="PLW13_09185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQY70589.1"
                     /translation="MAERLAIKDTLTETRIFSKRVVIAAAGVLALAAVLLGRYFHLQI
                     VDHEKYRTESDRNRIHARAVPPRRGLIFDRNGTLLADNRPIFNIMVTRERALDIDAML
                     AELRTIIEVTEDDEQRFRKQLARHLPFEAVPLKFDLNEEEIARLAVNRYRLPGVEVEA
                     ELVRHYPYGELLAHAVGYVGRINEQELAEIDRLDYSGTHLIGKNGIEKFYEDELHGSV
                     GYENVETNARGRALRVLDHTDPVRGQDLTLELDLEVQRVAYEALGSELGAVVAIDPRT
                     GGVIAIASAPSFDPNLFVSGISSADYAALRDSPARPLLNRTMQGQYPPGSTVKPMYAL
                     AGLYYGVNTPQSAVRDPGFYRLGGRGRKYRDWKKWGHGWSVDLDLAIAQSCDVYFYDL
                     AHRLGIERLHDFSVKFGLGSATGVDQTSERPGVMPSNAWKQKALGQPWYPGETLSAGI
                     GQGYVLMTPMQLAVMTATLANRGRHYRPHLLREVDGKPVTPELLHTVEIADPAHWDAV
                     VTGMENVVHSPRGTAKIINTSDLRYRIAGKTGTAQVVGIAQNASYNSAALRKTQRDHA
                     LFIGFAPADDPQIAVAVIVENGEHGSSAASPVARKVMDAFLLPRLPAPATEAAADDSA
                     AAAALPTMDGVAPAAPAQPAQEAAPSGDAGPSAGERTPQSAAPAREG"
     gene            11860..13008
                     /gene="rodA"
                     /locus_tag="PLW13_09190"
     CDS             11860..13008
                     /gene="rodA"
                     /locus_tag="PLW13_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQY70590.1"
                     /translation="MARGDFIRQMPESARDFGRKPTLYERLHIDLPLLGILFALCVYG
                     LVVLYSASGRQIDPVLRQGGFMAMGFAAMFAVAQLSAQTLRRWTPWAYLLAVLLLLAV
                     DIVGIGAKGAQRWLAIPGLPRFQPSEIMKLVMPMMVAGFLAATVLPPRFLHVCITLAI
                     VLVPTALVLVQPDLGTAVLIAAAGFIVLFLAGLSWRYVMAVVLAVATAAPLMWQFVLY
                     DYQKRRVLTLLDPDSDKLGAGWNIIQSKTAIGSGGVAGKGWLLGTQSHLDFLPEGHTD
                     FIIAVLAEEWGFRGVVLLLALYLALIGRALWIALRAQDTFSRLTVGGLSLTFFVYIVV
                     NMGMVSGLLPVVGVPLPLISQGGTALISLLAGFGIIMSVATERRRMLG"
BASE COUNT         1841 a         4528 c         4781 g         2012 t
ORIGIN      
        1 ggggtgaggc ggattccggc gaagcactgc ttcgccccgc cgaacgcccg aaatctacga
       61 tttcgggccg ggccccggag ggggattcac ggcgtgtccg cggcaccgat gggtgctcgg
      121 gcgccgatgg ctcgcgatac gcgggattgt cgggctcgtt atcccgagca cagacggttt
      181 tacaacaccc tctgtatctc cgcgccgcgt cctgcggcac aagggtccgc aagccaagag
      241 cgggcagcac agccagcgac gcaccgggcc gctcgcccgc aacctgatgg cctttgcgac
      301 aatctctgat gcccgacctg cggcatgtcg caccatgaac ccgcctacta ggctggtcgg
      361 agttccgttg gctcgagccg gtagcgcccc gcggcatcgc ggcggaactc cagccggcgc
      421 gtgatgcagg ccggcatttc ctcggcctcg tggctcacgt agaggatctg cgtgttcgtc
      481 tcgtggccga tgcgctcgat gagttccagc acgcggcggc ggttcgcctc gtcgagaccc
      541 gagcacggct cgtcgaggat caggatcggg gggttcttca ccatcgcgcg cgcgaccagc
      601 atcatgcgct gctcgccgaa ggacagcgcg tcgaagcgct cgcccgcacg tgccgacagc
      661 cccagtaccg ccagccattc gcgcgccagc cgcgcctgct gcggaccgca ggtgtcgtag
      721 aggcccagtg tgtcgaagaa gccgctggcc accacgtcga aggcgcgcgt gcgcgccggg
      781 taggacaggt gcagctggtt gctgacgatg ccgaagcgcc gcttgatctc ccataccgac
      841 tcgccgctgc cgcgcaggcg gccgaagagc ttcacgtgct ggccgtaggc gcgcgggttg
      901 tcgccgcaga tcagcgccag cagcgtcgac ttgccgcagc cgttcggccc cgcgatgcac
      961 aggtgctcgc ccggcgcgag gcgcaggctg acgccgtcca gcacctcgtg ctcgccgtag
     1021 tgcgcgtgca cgtcctccag ctcgatcagc gcgccggcac agggccgggc gggcgcggca
     1081 tcgggcgccg ccggcagcgg ccccggcgcg cagctcgcgc cagggccgaa caggcgctgt
     1141 gacgcgtcgc cctcgagcac ggcctcgcgc ggcccgcggt ccagcacctg cccgtggtcg
     1201 agcagcagca cgtggctgat gccgggcgcg atgtcctgcg gccggcgcgt cagcaacaga
     1261 atgtgcgtgg gcccgccgag caactcgtcg accagcgcgc gcaggacctg ccgcgaggcg
     1321 cggtcgacgc cttcgtaggg gttgtccagc accatcaggc gtggctccat cagcagggcg
     1381 cgggcgaaca gcgtgcggcg ggtctcgccg ctcgagagga agcggatgcc gcgctccatc
     1441 agcgcctcga tgccgagcat cgcggcgatc tcctcgacgc gttccgggcg gcgcgccgcc
     1501 gctgcgagca gcgcgcgcac ggtggtgccg gcgtcgacgg cgttctccag gtaatcgctg
     1561 atgtcgtggc gcgcgtcggc ggcgcacagc tcgcgctgcg cctcgaacga gacccaggcg
     1621 atccgcgccg gcgttaggtc ggcggcgagc gtgagctcgc cgctcgccgg atgcagtgcg
     1681 cgggcgatca gttgcgccag caccgtcttg ccggcgccgt tcgggccgag cacggcccac
     1741 tgctcgcccg gcgcggcgct ccagtcgagg ccgcgcagga acagtttgcc gtgcaggcgc
     1801 acggaaacgt cgcgaaagcg caaccaggat gagaccgggg cggtgtggtg gtgctgcatg
     1861 cggtgaaaat ccctcgtgga tcggctattg tccggtctcg tcgccgaaga gcaagcgcga
     1921 cgatcgcagc ggtagatcca gatggccggt aaacagcagg aaaacagtgt gagccccgcg
     1981 ctgcgcgaca tcggcgcggc ggtgcaattg ctggcggaac accgcccgcg acgcaagctg
     2041 gtgcgccgta tcctggcgcg ctccgcggtg ggtctctatc tcagcgatca cttgcccggc
     2101 gcgctctcgg tgctgatgat caagcgtgcc gagcgcgagg gcgacccctg gtccgggcac
     2161 atggccttcc cgggcgggcg caaggaccgc ggcgaccgca acacgctggc gaccgccaag
     2221 cgcgagacgc tggaagaggt ggcgctggat gccgatcgcc atgcccgcct cctcggcagg
     2281 ctttccgaca tcgccacgca gccgggcctg cgcggcgccc cgatgatcat cacgccctac
     2341 gtgttccagt tgcattcgtt gccgccgctc gtgcccaacc acgaggtggc cggcctggtg
     2401 tgggtgccgc tgccgttcct ggccgatcgc gccaaccgtg aaaagatgcg cctggcaccg
     2461 aacggggtgc cgatcgagct gccgtgctac atgttcggcg agcaccggat ctgggggctg
     2521 tcgctgatga tgctcgacga actgctggat gcgctggcct gagccggacc gatgatgcta
     2581 gagtcgcgcc cgctctgggg cacccccagc gcgcgtgcca gccccccttt gtccccccga
     2641 tcgaaccgct gcctgcagct tgcccggggc gccgcgatgg catgggcgat gccgtatccc
     2701 gatgccgtac cgcgaggatg attgcgcacg atgaccgaac gatggctgac actgtgcgag
     2761 cggctgctgc tggcgcggcc ctggcacagt ctgctggtga tcctggcgct gctgctgatc
     2821 gtcggcgcgc atatccccga tctgcgcttc gacgccgcgc ccgacacctt gctggcggat
     2881 gacccggcga gcggcttcgc cgccgagatt gccgcggagt tcggcccgca ggaattcctc
     2941 cttgtcacct accggccgca gcgcgacgac ctgatggccg agcgcgtgct ggagcgcatc
     3001 gcgcggctgc gcgcggcgct ggcggcggtg gcgggggtgc acgacgtgag cagcatcctc
     3061 gaggtgccgc tgctgttcag tccgccgatt cgcttcgccg acatcggtgg ggagttgcag
     3121 cggctgcagc ataccggtgt ggacaccgag ctcgcgcgcg cggaattcag gcaaagcccg
     3181 ctgtatcgcc agtcgctgct ctccggtgac ggccaggaaa ccgcgctgcg cgtcacgctg
     3241 gagcccggag ccgcggaggc cctgctgcag ggtcagcagc gattgctcga cgatctgcag
     3301 gcggtgctgg agcggcatcg cgacgaggcc accctgttcc tcggcggccc gacgctcacc
     3361 gccgccgcga tgccggcctt cgtgcagcgg gatctcgtgc gcttcggggc ggcgacgctc
     3421 gccgtgatgg cgctggtgct ggtcgcgctg ctgcgccggc tgccgtgggc actggttccg
     3481 cccgccgccg ccaccgcgtg tggcgtgctg ctgttggggc tgctcggctg gagcgggcgc
     3541 ccgctcggcg gcgtctcggc ctatgcgctg ccggtcgcgc tggtcgcggc ggcgaccatg
     3601 gcgctgcgcc tggtctgcgc ttacggtgag tgcctggcgc ggcagcccgc gcgtgcgccg
     3661 cgcgaagtcc tcggcgagac gctgcgcgct gcgctggcgc cgctgctcgg cggcgctgcc
     3721 gtggccgccg tggccgctgc tgcgctcgcc tcgagcccgc tggtggcggt cgccgcgctg
     3781 ggcgtgacgg ccgcatccgg tatcgcgctg gcggctctgg tcgcctgcgt gctggtgccg
     3841 gtgctcctgc tgctgggggc agggggcggg cgccagcccg tggcgcccgc gcgtgccggc
     3901 gtgttgctgg gggggctggc cgggcgctac ggcatcagcg tggcgctgct ggcactggcg
     3961 ctgctggccg gcggacttgc cggcatgcag aagctgcagc cggaggatcg cctgctcgag
     4021 cgctttggcc catcgagcga gctgtttcaa ggcatgctga atatcgagcg cgagttcggc
     4081 ggcgccgaaa cgctggcgat cgtgctcgag tcggatacgc cacagcaggc gggtgccggt
     4141 tccgcgaacc cgtggtacag cgcgcgcggc ctgcagcgcc tgcggcaggc gcacgactac
     4201 ctggacgcgc tgccggagag cgccgcggtg cgctcgctgg cgttgttcgg cgcgaccgcc
     4261 accgagctgc tggacggccc ggccgatgac aagcaactgg ctgcggcctg gcgaggcatg
     4321 ccggccgcgc ggcgcgcatt gttgctcgat ccctggctgt cggcctccac ggcgcggacc
     4381 ctgatcgtgg tagggctgaa cgcgagcggg ccggcgccgg cgcgccatga gctgatcgag
     4441 cgcatccgcg cgcagctggt gggagagctc ggcttcgcgc ccgggcaggt gcgcatcggc
     4501 gggctcgcgg tgctggaggg caatgcgctg cgcacgctgt ggcgcaccca gttcatcgcg
     4561 gtcgcggcgg cctgcgccgc gctcgcgctg ctggtgctgg cgcgttggcg ttcgccgctg
     4621 ctggcgctgc tggcgctggc gccgagcctg tgcacggccg tggtggtcgg cggtgcgatg
     4681 gggtggttcg gcgtgccgct cggcacgttg tcggtggcgt tcgcggcgct cgcggcggcg
     4741 agctgcgccg ccgccaccct gcactgcctg tggcgcgtcg gcgccgatcg ggcgagcgcg
     4801 ccgcagcgct gcggcgccgg tacggcggcg ctgctgtgct acattccgct gggcgtcggc
     4861 gcgccgtttg cgctgctcgc ggcatcggat ttccgcccga gcgcgcattt cgggctgctg
     4921 tgcccgctgg cgctggcggc tgcctcgggc tttgcggcgc tgctgccgcc attgttgcgc
     4981 tggtccaggc ttcccgccgg ccgcaggccg cgggcgggcg acgacgattt ccattccgcg
     5041 gacgcatgag gcgcgcatga ataccgaaga gctgccgttg ttcccgctga gcgccgtgct
     5101 gctgcccggc gggcgcgtcc cgctgcagat attcgagccg cgctatctcg atctcgtgag
     5161 ccgctgcatg aagaccgatt cgggtttcgg catcgtgcgc atcaccagcg ggcacgaggt
     5221 gatcatcgcg cagggcacca cgccgccgca gaccgcgccg atcggcaccc tggcgcgcat
     5281 cgtcgactgg gatgcgctgc ccgcggggcg cctgcgcatc acggtggagg gctcgcgccg
     5341 attccgtgtg gcgaccacgc gcgtgcaggc cgacggactg ctgtggggcg aggtgcagtg
     5401 gctggcggaa cccgggaacg ggccggtcgc ggacgagcac cagttcctgc aggacatcat
     5461 gagcgggttg gccgaacacc ccgcgctggc gcgcctgggc gtgcgcggca gctgggccga
     5521 ccgtgcgagc ctttcctggg cgctggcgca atacctgccg ctggaggaag ccgagcgcta
     5581 cgcggtgctg gccgaacccg aaccgctggc gcggctcgcg tacctggagc gcctcgtcaa
     5641 ggcgatgggg agttcctgag gccccgctgc gtcacgatcg cccgctccac cagcaccagc
     5701 cggtcgttgc cgaagtacat gtcctcgccg tcgaggaaca gggtcggcgt gccgaagcct
     5761 ccgcgtgcca cgagctcctc ggtgttgcgg cgcaggcgct ccttcagcgc cggctgctcg
     5821 atgcgctcga ggaatgccgg gccgtcgaag cctaccgcgg cagcgatgtc cgccacgacc
     5881 tgcggctgcg agatatcggc caggtcgcgc cagtagcgct ggaacacggc gcgcgcgtag
     5941 ggcaccagcc ggccctcgtc gagcgccacc agcgcgccgc gcatcgcctt cacgctgttc
     6001 accgggaaca ccgcgggcca gccgatctcc agcccgtagt agcgtgccca gtccgcgagg
     6061 tccttcatcg tgtactgcca gcgacgcggg ttcgggttcg cgcgcgcggc gtagacctcc
     6121 tggttcaccg cgttgaacac gccgccgacc aggatcgggc gccagtcgat cgccacaccg
     6181 tggcgctcgg cgatcggctg gacgccctcg aaggcgaggt aggtccaggg gctggagcaa
     6241 tcgaagaaga attccagttt cacgtcgtga tctccctagt cgtgaggcgg aaccgggccg
     6301 ccgccgttgc cggcgaggaa gcgccggtgg atccagaata cgagacccgc ggccgccagc
     6361 gcgaccacga ccatcgtcag caggatccgc ggcgaggcca ggtgcgtttc caccgcggcg
     6421 ccgaggtagt agccgggcag gatgtagacc ggtgcccagg ccagcgccga gaccgcgttg
     6481 atcaggaaga acgggcgcgc ccgcatgttg aggatgcccg ccaccagcgg gatcaccggg
     6541 cggatcggac cgacgaagcg gccgatggcc acgctcttgg caccgtggcg cgcgaagaag
     6601 cgctcgccat tggcgatcca ctcgggattg cggcgaaacg gccaggcgcg cttgatgtcg
     6661 tcgtggaaga tgcgcccgag caggaagctg accccgtcgc cggccacggc gcccgcgaac
     6721 gccgcgagca ggcattcggg caggccgagc gcgcccgagc cggccacgaa ggcagccacg
     6781 ccgagcagcg ccacgccggg caccgcgatg ccgaggatcg cgaaggattc cgcgaaggag
     6841 atcagcgcga tcgccgcggc tacccaggcg ggatgctgtt ccagccaggg ctgaagggcg
     6901 ctcgcatcga aggaggcaaa gggcatcggc gacggtattc ctgcacgggt tgcggtcacc
     6961 cgccgtcggg ccggtgccgg ggcggcgcgt gcggcgggcg gagggcgggg atataatgcc
     7021 acgcctttgc gccaatcccg gcgcgtctca ctttcattcg gaaccggaca tcgcatggat
     7081 atcgggtcgt atatggccga cgtcggccgc gaagcgcggg ccgcctcgcg gctgatggcg
     7141 cgcgccacga cgcaggccaa gaacaacgcg ctgcaggaag cgggcgcggc gatcgcggcc
     7201 gcgcgtgccg aactcgtcgc ggccaatggc gacgacatga cgcgcgcggc ggcgcagggg
     7261 ctggacgccg cggcgctgga ccgcctgaag ctcaccgatg cgcgcatcga cgccatgatc
     7321 gaggggctcg agcaggtcgc cacgctgccc gaccccgtcg gcgccatcac ggacctcgcc
     7381 taccgcccca gcggcatcca ggtcgggcgc atgcgcgtgc cgctcggcgt ggtcgggatc
     7441 atctacgagt cgcgcccgaa cgtgaccgcc gacgccgcga gcctgtgcct gaagtcgggc
     7501 aatgccgcga tcctgcgcgg cggctcggag tcgctggcat cgaaccgcgt gatcgcctcc
     7561 tgcatgcacc gcgggctgcg cgccgcgggc ctgccggaac gtgcgctgca ggtgatcgcg
     7621 acggcggacc gcggcgcggt cgggctgctg atcacgatgc ccgaatacgt cgacgtgatc
     7681 gtgccgcgcg gcggcaaggg gctgatcgag cgcatttccg ccgaggcacg ggtgccggtg
     7741 atcaagcacc tcgatggcat ctgccacgtc tacatcgacg aggcggcgga cctcgacaag
     7801 gccttcgcgg tggcgctgaa cgccaagacg cagcgctacg gcacctgcaa caccatggaa
     7861 acgctgctgg tggcggcccc cgttgccgcg gcgatcctgc cgcggctcgc aacggcttac
     7921 accgagcacg gcgtggagct gcgcggctgc gcgcgcacgc gcgagctgct gccccgggcg
     7981 ctggcggcca cggaagagga ctggggcacc gaatacctcg ccccgatcct cgccatccgc
     8041 gtggtggccg ggatcgacga ggcgatggag cacatcgcgc gctacggctc gcagcacacc
     8101 gacgcgatcg tgaccgagga ctacagccgc gcgcggcgct tcctgcgcga ggtcgattcg
     8161 agttccgtga tggtgaacgc ctcgacgcgc tttgccgacg gcttcgagta cgggctcggc
     8221 gcggagatcg gcatttccac cgacaagatc cacgcgcgcg gcccggtcgg tctcgagggg
     8281 ctgacctcgc agaagtacgt ggtgctgggc gacgggcatg tgcgccgctg aacacgcgcg
     8341 ggacgccggg tgaacgacag cgcggcgcgc cgcatcggta tcctcggcgg aaccttcgat
     8401 ccggtccaca acggtcatct gcgcaccgcg ctcgagttgg ccgagctcct cgcgctcgac
     8461 gagttgcgcc tggtgccgtc cagcgtgccg ccgcaccgcg gtgcgccgct cgccaccgct
     8521 gccgagcggc tgcgcatgct gcagctggcg accgacgcgc accccggttt cgtggtcgac
     8581 gaccgcgagc tcgtgcgcgg cggtgcctcg tacaccatcg acacgctgcg ctcgctgcgc
     8641 gcggagctcg gcgcggcggc gcggttatgc ctggtcgtcg gcctggacgc cttcgccgcg
     8701 atcgatacct ggagggagtg gcgcgcgctc ggcgagcacg cgcacatcgt ggtggtgcag
     8761 cgtcccggcg tcgcgccggc gctgcacgag gcggttgcgc gctgggcctc ggcacggctc
     8821 gccgccgatg ccggcacggc gctgtgcgcc gcggcgcacg gtgccgtgct gcgcctggag
     8881 ctgacgcagc tcgcgatctc cgcgacccat atccgggaac tgctcgcgca ggggcgctcg
     8941 ccgcgttacc tgctgccgga ggcggtgctc gactacatcc gggaacaccg catctacaca
     9001 cgcgcccctg cgcacacggg agatacagat tgagcaagaa ggattcggcg gaactggccg
     9061 cgctcgccgt gcgcgccatc gaggatctca agggccagga catcgtgcgc ctggacgtgc
     9121 gcaagctcac cgatgtcacg gattacctgg tcatcgccac cgggacctcg tcgcgccacg
     9181 tcaagtcgat cgccaacaac gtgctggagg aactgcgcaa tgccggcgag cgcccgatcg
     9241 gcgtcgaggg caccgaggcc ggcgagtgga tcctggcgga tttcggcggc gtggtggtgc
     9301 acgtgatggg cgccgagccg cggcgcttct acgccatcga gaagctctgg tcggaaatgc
     9361 cggagacgcg ctgatggcgg cgcgtctgcg cattctcgcg gtgggcacgc gcatgccggc
     9421 ctgggtgcag gccggattcg ccgagtacca caagcgcata ccgcgcgatt tcgccgtaga
     9481 actgcgcgag atcccggtgt cgcgccgcga gggtgcgcgc gcggtggccg aggagggcga
     9541 ggccctgctc gcggcgctcg gcgccgacga gcacgtggtg gcgctcgagg tcggcggcga
     9601 gccgtggagc acaggggacc tcgcgtcccg gctgcgcgtc tggcgcgacg agggccggcg
     9661 cgcggccttc ctgatcggcg ggcccgacgg tctcgcggat gcgtgtcgcg cgcgcgcggc
     9721 gagccagtgg tcgctctcgc ggctcacgct gccgcatccg ctggtgcgca tcgtgctgat
     9781 ggaacagatc tatcgcgcgt ggagcatcct cggcgcccat ccgtaccacc gctgagcgcg
     9841 agatggccga gcgactcgcg ataaaggaca ccctcaccga gacgcgcatc ttcagcaagc
     9901 gcgtggtgat cgccgccgcc ggcgtgctcg cgctcgccgc ggtgctgctg gggcgctatt
     9961 tccacctgca gatcgtcgat cacgagaagt atcgcaccga gtccgatcgc aaccgcatcc
    10021 acgcgcgcgc ggtgccgccg cgacgcggcc tgatcttcga tcgcaatggc acgctgctcg
    10081 ccgacaaccg gccgatcttc aacatcatgg tcacgcgcga gcgcgcgctc gacatcgacg
    10141 cgatgctggc ggaattgcgc acgatcatcg aggtgacgga agacgacgag cagcgctttc
    10201 gcaagcagct cgcgcgccac ctgcccttcg aggcggtgcc gttgaagttc gacctgaacg
    10261 aggaagagat cgcgcgtctc gccgtgaacc gttatcgcct gcccggggtc gaggtggagg
    10321 ccgagctggt gcgccattac ccctatggcg agctgctcgc gcacgcggtg ggttacgtcg
    10381 ggcgcatcaa cgagcaggaa ctcgcggaga tcgaccgcct cgactacagc ggcacgcacc
    10441 tgatcggcaa gaacggtatc gagaagttct acgaggacga gctgcacggc agcgtcggct
    10501 acgagaacgt cgagaccaac gcgcgcggac gcgcgttgcg ggtgctggac cacaccgatc
    10561 cggtgcgcgg gcaggacctg acgctcgagc tcgacctgga ggtgcagcgc gtcgcctacg
    10621 aggccctcgg cagcgagctc ggcgcggtcg tcgccatcga tccgcgcacg ggcggcgtga
    10681 tcgcgatcgc cagcgcgccc agcttcgatc cgaacctgtt cgtgtcgggc atcagcagcg
    10741 ccgactatgc ggcgctgcgc gattcgccgg cacgcccgct gctgaaccgc accatgcagg
    10801 ggcagtatcc gccgggctcc acggtgaaac cgatgtacgc actggccggg ctttactacg
    10861 gcgtcaacac gccgcagagc gcggtgcgcg atccgggttt ctaccggctg ggtgggcgcg
    10921 ggcgcaagta ccgcgactgg aagaagtggg gccatggctg gtcggtggac ctggatctcg
    10981 ccatcgcgca atcctgcgac gtgtatttct acgacctggc gcatcggctc ggcatcgagc
    11041 ggctgcacga tttctcggtg aagttcggcc tgggctcggc gaccggtgtc gaccagacca
    11101 gcgagcgtcc gggcgtgatg ccatcgaacg cctggaagca gaaggcgctg ggccagccct
    11161 ggtaccccgg cgagacgctg agcgccggca tcggccaggg ctacgtgctg atgacgccga
    11221 tgcagctcgc ggtgatgacg gccacgctcg ccaatcgcgg caggcactac cgcccgcacc
    11281 tgctccggga ggtcgatggc aagccggtca cgcccgagct gctgcacacg gtggagatcg
    11341 ccgacccggc ccactgggac gcggtggtga cgggaatgga gaacgtggtg cacagcccgc
    11401 gcggcactgc caagatcatc aacaccagcg acctgcgcta ccgcatcgcg ggcaagaccg
    11461 gcacggcaca ggtggtgggc atcgcgcaga acgcgagcta caactcggcg gcgctgcgca
    11521 agacgcaacg cgaccacgcg ctgttcatag gattcgcgcc ggccgacgac ccgcagatag
    11581 cggtcgcggt gatcgtcgag aacggcgagc acggcagctc cgccgcctcg ccggtggccc
    11641 gcaaggtgat ggacgccttc ctgctgccgc gcctgccggc gccggcaacg gaggctgcgg
    11701 cggacgactc agcggcagcg gctgcgctgc cgaccatgga cggtgtggcg cctgccgcac
    11761 ccgcgcaacc cgcgcaggaa gcggccccgt cgggtgatgc cggcccatcc gccggtgagc
    11821 gcactccgca atccgccgcg ccggcacgag agggctgaga tggcacgcgg cgatttcatc
    11881 cggcagatgc ccgagtccgc gcgcgacttc gggcgcaagc cgaccctcta cgagcgcttg
    11941 cacatcgacc tgccgctgct cgggatcctg ttcgcgctct gtgtctacgg gctggtggtg
    12001 ctgtacagcg cgagcgggcg ccagatcgac ccggtgctgc gccagggcgg gttcatggcc
    12061 atgggctttg ccgcgatgtt tgccgtggcg caactgagcg cgcagacgct caggcgctgg
    12121 acgccatggg cctatctgct ggccgtgctg ctgctgctgg cggtcgacat cgtcggtatc
    12181 ggggccaagg gcgcgcagcg ctggctcgcg attcccgggc tgccgcgatt ccagccgtcc
    12241 gagatcatga agctggtgat gccgatgatg gtggcgggct tcctcgcggc caccgtgctg
    12301 ccgccgcgtt tcctgcacgt ctgcatcacg ctcgcgatcg tgctggtgcc gaccgcgctg
    12361 gtgctggtgc agcccgatct cggcaccgcg gtgctgatag cggcggcggg gttcatcgtg
    12421 ctgttcctgg ccgggctcag ctggcgctac gtgatggcgg tggtgctcgc cgttgccacc
    12481 gcggcgccgc tgatgtggca gttcgtgctc tacgactacc agaagcggcg cgtgctgacc
    12541 ctgctcgacc cggacagcga caagctgggg gcggggtgga acatcatcca gtccaagacc
    12601 gcgatcggct ccggcggcgt ggcgggcaag ggctggcttc tgggaacgca gtcgcatctc
    12661 gatttcctgc ccgaaggcca caccgatttc atcatcgcgg tgctcgccga ggaatggggc
    12721 tttcgcggcg tcgtgctgct gctggccctg tatctcgcgt tgatcgggcg ggcgctgtgg
    12781 atcgccctgc gcgcccagga caccttctcg cgcctgaccg tgggaggtct cagcctgacc
    12841 tttttcgtct atattgtggt gaacatgggc atggtttccg gcctgttgcc ggtggtcggg
    12901 gtgccgctgc cgctgatcag ccagggcggc accgccctta tatcgctgct cgcgggtttc
    12961 ggtatcatca tgtccgtcgc caccgaacgt cgccgcatgc tgggctgacc gtgctcccgg
    13021 cagcgcgacg ccatcccggg tcctacaggg gttttgcttt gtttcgtgtc ctgatcctct
    13081 tcattgccct gctcgccggg ccggccctgg catccggtta cacggatcac gcgggctatg
    13141 ccgccttcga ggcgcgcatg gt
//
LOCUS       DAORUQ010000089        13129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4687_length_13129_cov_5.613890, whole genome shotgun sequence.
ACCESSION   DAORUQ010000089 DAORUQ010000000
VERSION     DAORUQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_13129_cov_5.613890"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLW13_09195"
     CDS             complement(<1..392)
                     /locus_tag="PLW13_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70591.1"
                     /translation="MALFSAAAGAGELYLRNGARIPGELRSVDGRNIVWRAELIGDIK
                     VETKSIASVDSTTAARLQVGASDALENCHLSGDANNVALRCTEGTTATAQWRDIETAE
                     AESSGKVTASLTRERGNSFSDDYEVDARA"
     gene            538..1812
                     /locus_tag="PLW13_09200"
     CDS             538..1812
                     /locus_tag="PLW13_09200"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQY70592.1"
                     /translation="MPDSSHCAADDTALEPAAGRTSRDGVLGHDAMAARLAAISIELN
                     NHEALWRALAFREPVLAWEAAHPALAAALRALSLETAEALHADPRACADWLARWLPVE
                     RWRALAEVGAWPRHALRPWPREFQRDVPGRKWAQIEAFAGAVRDGGHACIDWCAGKGH
                     LARAVSQQWNQRAVTSLERDEALVAAGAALARRARLPVVPHACDVLSEAVLPWLRAPH
                     HALALHACGGLHLRLLHAGAAARLPAISCAPCCYHLGAQAGRLVISVAGHTHAPRLRP
                     EDLRTAVQETVTAPGHARRHRCRVQQWQLGFDLLQREVRGVDAYLPLPPIDAATLAGG
                     FAAWCRALAERKQMRVPEDVDLARVECAGQERFRVVTALDLVRHQFRRLLELWLVLDR
                     ALYLSEQGYLVGVGEFCPRELSPRNLLIDARL"
     gene            complement(1843..3273)
                     /locus_tag="PLW13_09205"
     CDS             complement(1843..3273)
                     /locus_tag="PLW13_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70593.1"
                     /translation="MSHKVTLDETIEVARPLHEVFAYVSEFSRIEQWDPAVARGTRLT
                     PGAPGLDSEYRIDMKAGFSLHYRVIEWEPGRRMLMTVDSRLFTAREEILFSATARGTR
                     LRYIATFDFPAPLAAMSRVFPSVMDRVGKNTMEGLQRALEDNFEPPRESGATALADRL
                     LLPGLCRFTRLGYRAARKRWKPVSAWLGGRHALVTGATSGIGFAAARELAALGARVTI
                     VGRDPAKTEQAARAIRDSTGNPQVAFDIADLSLMADVNALAERLLARGEPLDILVNNA
                     GALFNPRQLTAEGLEKSFALLLLSPWLLTERLHPLLAKAGSARVVNVLSGGMYSQRID
                     VDDLQSRNGRYSGAVAYARAKRGLMILTEEWAEKWRADGIVVNAMHPGWADTPGVESS
                     LPAFYRSTRAALRSPEEGADTVVWLGAATEAGKVTGRFWLDREQHPSHVFRHTRETPE
                     ERRRLLEALGGYLTSTRTARRSRRQA"
     gene            complement(3270..4067)
                     /locus_tag="PLW13_09210"
     CDS             complement(3270..4067)
                     /locus_tag="PLW13_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70594.1"
                     /translation="MKHLVFAALACATTMGSAWAQPVVPEIIEGVALRLPERTEVYHE
                     RHEIGTLGHRIEYRAPDGTLIARNALDYHCSESAPAFEQHDLRSDSRIGARWQHGEYL
                     LLRGGEMRALEPAATLVASSGFDRFVRANWEALAAGDSIDVDFALPARLQTLRLRMER
                     TEAPEELPGSALWLRIVPVQPLLRAFVDPIELAYDSGRRLVLYRGLSNLDGADGKALN
                     VEIRYRPVSGEDRAREREIAATAPAMTEPAADAPRTPDQLHCSRENS"
     gene            complement(4064..4582)
                     /locus_tag="PLW13_09215"
     CDS             complement(4064..4582)
                     /locus_tag="PLW13_09215"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HQY70595.1"
                     /translation="MTNARFLNAIAYQCGWLACVAGGNAWALPAGALILALHGWLMER
                     SARGWAVIAVAALLGLAMDLGWQQLGLLEFHGTLGAGIPPWLVVLWLLFATTFAHSLA
                     WLHERLALGALLGAVLGPLSYVAGVELGAATTRFPLWQLALAMAPAWALLLPLLLFLA
                     RAPRAVATEALS"
     gene            complement(4639..6042)
                     /gene="phrB"
                     /locus_tag="PLW13_09220"
     CDS             complement(4639..6042)
                     /gene="phrB"
                     /locus_tag="PLW13_09220"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQY70596.1"
                     /translation="MSTVVWFRSDLRADDNSALAEAARDGARVTGLFLLTPAQWRAHD
                     WGARKQVFVWRHVLALRERLAALGIPLWVRLVPEFAGAPAAVAEFARAQAATAVYANA
                     EYAVNEVARDRLVAEALRAQGTAWRNLHDFTVLAPGTVRTQQGAPFKVFSPFRRAWLA
                     QVRGEPVDCVAAPTARAAVEPPPLPDWEPPVATLDPDWWPIGEVAARARLADFVEHGL
                     ARYHEDRDVPALDGTSRLSPYLAIGALSVRRCIEAALAHNHGEWDSGSRGAQTWLSEL
                     VWREFYTHILAEFPRVSRARPFRPETEAIAWREPGVDFTRWCEGRTGYPLVDAAMRQL
                     AATGWMHNRLRMVTAMFLTKYLLVDWRHGERFFCEQLVDIELAANNGGWQWSASTGTD
                     AAPYFRVLSPVRQAERFDAEGAFVRRWLPQLAALPAKAVLQPGHPALLATGYPEPMVE
                     LKGARERVLLAFRGLAP"
     gene            complement(6039..7301)
                     /locus_tag="PLW13_09225"
     CDS             complement(6039..7301)
                     /locus_tag="PLW13_09225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQY70597.1"
                     /translation="MSTARTRELELPQTARLPWSARIARGKIFERIAALPRGRLVIED
                     AEGTWRFGSASAREELVAHVQVTDSAFYHELASGGSVGAAESWVRGHWKTPDLLKVMQ
                     LMAANIDLLNAIDDRSSALERVALRALHLLNRNSKAGSRRNIEAHYDLGNEMFALFLD
                     PTMMYSSAIFPHAGATLEEASRAKLARICRRLELGPDDHLLEIGTGWGGMAEYAARHT
                     GCRVTTTTISRQQYDYATQRIAAAGLSERVTVLLQDYRELEGQYDKLVSIEMIEAVGH
                     RYLPQYFAACNRLLKPEGLMLLQSILMPDQRYARSRHNVDFIQRYIFPGGFLPSTGAI
                     ATLTGTHTDMQLIDTLDITAHYADTLAAWRRRFHAGTEAIRALGYSEDFVRLWDYYFC
                     YCEGGFRERVIGTAQFLLAKPQYRPPAA"
     gene            complement(7298..8074)
                     /locus_tag="PLW13_09230"
     CDS             complement(7298..8074)
                     /locus_tag="PLW13_09230"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HQY70598.1"
                     /translation="MVSTSAIYTGEVRHRRFTPATHAFRYRMCMLLLDVEKLPTLFAG
                     SRLWSAARPAPGWFRRKDFLGDPKRPLAECVRELVAAHTGRRPAGPVMLLANLRYFGV
                     LMNPISCYYCYGADGETLQAVVAEVTNTPWGERHAYVLPVEDNAGVLRAEFAKSFHVS
                     PFNPMDMRYRWMSRTPGERIGIHLENHRDGAKVFDATLSLQREPLDARALRRLLLVRY
                     PLMSLRILAAIYFEALRLFAKRVPFHSHPPASMPIKESTG"
     gene            complement(8068..9318)
                     /locus_tag="PLW13_09235"
     CDS             complement(8068..9318)
                     /locus_tag="PLW13_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70599.1"
                     /translation="MKIAIVGAGIAGLSAAWLLSRRHEVTVFEGEERIGGHTATIDVS
                     LDGERHAIDTGFIVFNDRTYPNFIRLMDELGVASQPTAMSFSVSSELEGIEYAGSNLN
                     SLFAQRGNLLRPAFWRMLRDILRFNRSAAADLEQGRIPEGMLLGDYLARNGYSREFRD
                     WYLVPMGAAIWSSGTRGMAEFPVEFFVRFFRNHGLLTVTDQPQWRTIRGGSREYLGPL
                     TRRFAGAIRTGDPVERITREADGVSVRTRAGFSERFDHVVLGCHSDQALALLGDPSAE
                     ESGILSAIPYQDNDVALHTDTRVLPRAPRAWSSWNYRLRPGADELPVLSYDMNILQRL
                     ESRHTFCVTLNDTATIDPSRILGRYRYSHPVFSVEGVRAQQRWAEINGVRRTWFCGAW
                     WANGFHEDGVASALRVAEALGAPW"
     gene            9593..10174
                     /locus_tag="PLW13_09240"
     CDS             9593..10174
                     /locus_tag="PLW13_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY70600.1"
                     /translation="MNISAVPVDREPDAWTTRLEAVARERDRQAYAELFRHFAPLLKS
                     FALSSPGMSSNALAEELVQEVMLKIWNRASTFDAGKASATTWIYTLARNCRIDLLRKK
                     FRVREEVDVDELWDLGTEDDVLEDEVYQKRIEVDVRESLGGLPAEQRQIITKVYMEGK
                     SHSEVAEELGLPLGTVKSRVRLAMSKLKVMLDR"
     gene            10171..10854
                     /locus_tag="PLW13_09245"
     CDS             10171..10854
                     /locus_tag="PLW13_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HQY70601.1"
                     /translation="MSAATMTHYHPDQFLLNDYVAGSLPGAVALPIAVHLEYCAQCRE
                     EVAQLSRLGAELFSELDPVPVGDDAFARLLDQLDGKGAVPVTPSAPRRPARERDGLPR
                     ALRSLAPAGVEELDWKRISTSLRASRLGFGDPQREVALHHIRAGGKVLAHGHAGSEIT
                     VVLQGSFSDQDGRYAQGDFLVRGPGDEHRPVAAADSDCLCLAVLDAPIRLGGLLGRLA
                     NPFLRIHPR"
     gene            10867..11544
                     /locus_tag="PLW13_09250"
     CDS             10867..11544
                     /locus_tag="PLW13_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70602.1"
                     /translation="MSDDARTDGIGMEKDADAGQSQQEPGAAPSSAPQPERRDSDKRL
                     VLIVSHLLVAMLVLSMWAAADTWQAVTGLALASLLSVLTAFLAGFVLGTLVHEWGHFL
                     GARRAGASYTIPGKPGLFVFNFDFARNSASQFLTMSYGGQLGGALAVLLLWLAVPTDT
                     AGRAMLVAATGGGAVFAAAIEWPVIRAVRAGGEPAAQLGKIDRAVLSSSALKGLVGTA
                     LLWLILL"
     gene            complement(11757..12932)
                     /locus_tag="PLW13_09255"
     CDS             complement(11757..12932)
                     /locus_tag="PLW13_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY70603.1"
                     /translation="MSAAARTDGPAMARSVRETVMRNAGANEQARTLNAETVEALWNS
                     GLMQFMNPHCAGGHEPSFRELIDTWQELAWQDGSVGWIGIANFPGAAFAAAYLPEAGY
                     DEVFVQHGNRSTVGGQFAPNGLGQRVEGGYRLSGSWNFGSGTGHSNFVVGGFIPMHGS
                     EMQLGELGMPEMLVAVMPREQVNFTDGWFVQGLKATGSYDYNVQELFVPEARLFPLFT
                     RAPLRGGPLYRFGIMPITAAGHAAWALGVARSALDDVKTLALEKVRMGDMSTLAHRPT
                     FQRGLAHHEGMWRAARLLITDTFEGFETSVAAGAAVTPAMRAEMRLAATYATEACREV
                     VQWAHLAAGTTAIREGNRLERAFRDIYTGTQHTFIGEKTYTDAAQIMLGLIPDSPAL"
BASE COUNT         1926 a         4519 c         4674 g         2010 t
ORIGIN      
        1 gcccgcgcgt cgacctcgta gtcgtcggag aaactgttgc cgcgctcgcg cgtgagcgag
       61 gcggtgacct tgccgctgct ctcggcctcg gcagtctcga tgtcacgcca ttgggcggtg
      121 gccgtcgtgc cctcggtgca gcgcagcgct acgttgttcg cgtcgccgct gaggtggcag
      181 ttctccaggg cgtcgctggc gccgacctgc agtctcgccg cggtggtgct gtcgacggag
      241 gcgatcgact tggtctccac cttgatgtcg ccgatcagtt ccgcgcgcca cacgatgttc
      301 ctgccgtcga cgctgcgcag ttcgcccgga atccgtgcac cgttgcgcag gtacaactcc
      361 ccggcgccgg ccgccgcgga gaacagcgcc atgcaaatcg tcacaatcca cgtcccgcgc
      421 atcgccgctc cctcgctcgc accatatcga atcatgataa caacgcgccg atgtgatttc
      481 tccagccgtt caccgccgtc gttgacaagc cacggcggcg cctcctaccg tgcatccatg
      541 cctgattcca gccattgcgc agccgacgac acggcgctcg agcccgccgc cggccggacg
      601 tcacgtgacg gcgtgctcgg tcacgatgcg atggcggcgc gcctggctgc aatcagcatc
      661 gagctgaaca atcacgaagc actgtggcgc gcgctcgcgt ttcgcgagcc ggtgctggcg
      721 tgggaagcgg cgcatcccgc gctggcggcg gcgttgcgcg cactgtcgct ggagacggcc
      781 gaggccctgc acgccgatcc gcgcgcttgc gctgactggc tggcccgctg gttgcccgtc
      841 gagcgctggc gggcgctggc cgaggttggg gcctggccgc gccacgccct gcggccctgg
      901 ccgcgcgagt tccagcgcga tgtgccgggg cgcaagtggg cgcagatcga ggctttcgcg
      961 ggcgcggtgc gcgacggcgg gcacgcgtgc atcgactggt gcgcgggcaa gggtcacctg
     1021 gcgcgcgccg tgtcgcagca gtggaaccag cgggcggtga cgtcactgga gcgtgacgag
     1081 gcgctggtcg cggcgggcgc ggcactggcg cgccgcgcgc ggctcccggt cgtgccgcac
     1141 gcctgcgacg tgttgtcgga ggcggtgctg ccgtggcttc gggcgccgca ccatgcgctc
     1201 gcgctgcatg cctgcggcgg gctgcacctg cgcctgctgc acgccggcgc ggccgcgcgc
     1261 ttgcccgcga tcagctgcgc gccgtgctgc tatcacctgg gcgcgcaagc gggtcggctg
     1321 gtgatttctg tcgcgggcca cacgcacgcg ccgcgactgc gcccggagga tctgcgcacc
     1381 gcggtgcagg aaaccgtgac cgcgccgggg catgcgcgcc gccatcgctg ccgcgtgcag
     1441 caatggcagc tcggcttcga cctgctgcag cgcgaagtgc gcggcgtcga tgcgtacctg
     1501 ccgctgcctc cgatcgacgc ggcgacgctg gcgggcggtt tcgccgcctg gtgccgtgca
     1561 ctcgccgagc gcaagcagat gcgggtgccg gaggacgttg acttagcgcg ggtggagtgc
     1621 gcggggcagg agcgctttcg cgtcgtcacc gcgctcgacc tggtgcgtca ccagttccgg
     1681 cgcctgctcg agctgtggct ggtgctcgac cgcgcgctgt acctctcgga gcagggctac
     1741 ctagtcgggg ttggcgagtt ctgcccgcgc gagctcagcc cgcgcaacct gctgatcgac
     1801 gcgcgcctgt aggtcgccat gaatggcgcc ctacggcggg atctacgcct ggcgacgcga
     1861 gcgcctggcg gtgcgcgtcg acgtcaggta gcccccgagc gcctcgagca gtcgccggcg
     1921 ttcctcgggc gtctcgcgcg tgtgccggaa cacgtgactc gggtgctgct cgcggtcgag
     1981 ccagaacctg cccgtgacct tgccggcctc cgttgcggcg ccgagccaca cgacggtgtc
     2041 ggcaccttcc tcgggactgc gcagcgccgc gcgcgtgctg cggtagaagg cgggcagcga
     2101 gctctcgacg ccgggcgtat cggcccagcc gggatgcatc gcgttcacca cgatgccgtc
     2161 agcgcgccat ttttcggccc attcctcggt caggatcatc agcccgcgct tggcgcgggc
     2221 gtaggccacg gcgccggagt agcggccgtt gcggctctgc aggtcgtcga cgtcgatgcg
     2281 ctgcgagtac atgccgcccg agagtacgtt caccacgcgc gccgaaccgg ccttcgcgag
     2341 cagcgggtga aggcgctcgg tgagcagcca gggcgagagc agcagcagcg cgaagctctt
     2401 ttccagcccc tctgccgtga gctggcgcgg gttgaacagg gcgccggcat tgttgaccag
     2461 gatgtcgagc ggctcgccgc gggccagcag ccgctcggcc agggcgttca cgtccgccat
     2521 cagcgagagg tcggcgatgt cgaacgccac ctgcgggttg ccggtgctgt cgcggatcgc
     2581 gcgcgcggct tgttccgtct tcgccggatc acggcccacg atggttacgc gagcgcccag
     2641 cgcggcgagc tcgcgcgccg cggcgaagcc gatgcccgag gtcgcgccgg tgaccagcgc
     2701 gtggcgaccg ccgagccacg ccgacaccgg cttccagcgc ttgcgcgcgg cgcggtagcc
     2761 caggcgcgtg aagcggcaaa gccccggcag cagcaggcgg tcggcgagcg cggtggcgcc
     2821 cgattcgcgc ggcggctcga aattgtcctc gagcgcgcgc tgcaggccct ccatggtgtt
     2881 cttgccgacc cggtccatca ccgaggggaa gacgcgcgac atcgcggcca gcggcgccgg
     2941 aaaatcgaag gtcgcgatgt agcgcagccg ggtgccgcgc gcggtggcgc tgaacaggat
     3001 ttcctcgcgc gcggtgaaca gccgcgagtc caccgtcatc agcatgcgcc gcccgggttc
     3061 ccattcgatg acgcgatagt gcaacgagaa gccggccttc atgtcgatgc ggtactcgct
     3121 gtccagcccg ggcgcgccgg gcgtcagccg ggtgccgcgc gcgacggcgg ggtcccactg
     3181 ctcgatgcgc gaaaattccg acacgtaggc aaacacctcg tgcaacgggc gggcgacttc
     3241 gatcgtttcg tccagcgtga ccttgtggct catgagttct cccgggagca gtgcaactgg
     3301 tccggtgtac gcggcgcgtc agctgccggt tcagtcatgg cgggcgcagt tgcggcaatt
     3361 tcacgttcgc gggcgcggtc ctcgcctgac acgggccggt agcggatctc gacgttgagg
     3421 gccttgccgt cggcaccatc caggttcgac aggccgcgat acagcacgag acgccgcccg
     3481 ctgtcgtagg cgagctcgat cgggtcgacg aatgccctca gcaaaggctg caccggcacg
     3541 atgcgcagcc acaaggcgct gccgggaagt tcctcgggcg cctcggtgcg ctccatgcgc
     3601 aggcgcagcg tctgcagtcg cgcgggcagc gcaaagtcga cgtcgatgct gtcgcccgcg
     3661 gcgagcgctt cccagttcgc gcgcacgaag cggtcgaaac ccgaactcgc caccagcgtg
     3721 gcggcaggct cgagtgcgcg catttccccg ccgcgcagca gcaggtactc gccgtgctgc
     3781 cagcgcgcgc cgatgcgcga atcactgcgc aaatcgtgct gctcgaaggc cggcgcgctc
     3841 tcgctgcagt ggtaatcgag ggcattacgc gcgatcagtg tgccgtccgg cgcgcggtac
     3901 tcgatgcggt ggccgagcgt gccgatctcg tgccgttcgt gatagacctc ggtgcgctcc
     3961 ggcagccgca gcgccacccc ctcgatgatt tccggcacaa cgggctgtgc ccatgcgctg
     4021 cccatcgtcg ttgcgcatgc cagcgcggcg aacacgaggt gtttcacgac agggcctccg
     4081 tggcaacggc gcgcggggcg cgcgcgagga aaaggagcag cggcagcaac agcgcccacg
     4141 ccggcgccat cgccagcgcg agctgccaca gcgggaagcg ggtggtggcg gcgcccagct
     4201 ccacgcccgc cacgtagctg agcgggccga gcacggcccc gagcagcgcg cccagcgcca
     4261 gccgctcgtg cagccatgcc agcgaatgcg cgaacgtggt cgcgaacagc agccacagca
     4321 ccaccagcca gggcggaata cctgcgccca gcgtgccgtg aaactccagc aaccccagtt
     4381 gttgccagcc gagatccatc gcgagaccga gcagggcggc gaccgcgata acggcccagc
     4441 cgcgggcgct acgctccatc agccacccgt gcagcgccag gatcagtgcg ccggcgggca
     4501 gcgcccacgc gttgccaccg gccacgcagg cgagccagcc gcactggtag gcgatggcgt
     4561 tgagaaacct ggcgttggtc atgcaatgtc tccgagcgca gctagagaag gatgcagcat
     4621 gtcggcatga atgccggcct acggcgccag cccgcgaaag gcgagcagca cgcgctcgcg
     4681 cgcgcccttc agttccacca tcggctccgg gtagccggtc gccagcagcg ccgggtggcc
     4741 cggctgcagc actgccttcg cgggcagcgc ggcgagctgc ggcagccagc ggcgcacgaa
     4801 cgcgccctcg gcatcgaagc gctcggcctg gcgcaccggg ctcaacacgc ggaaataggg
     4861 ggcggcgtcg gtgccggtgg aggctgacca ctgccagccg ccgttgttgg ccgcgagctc
     4921 gatgtccacc agttgctcgc agaagaagcg ttcgccgtgg cgccagtcga ccagcaggta
     4981 cttggtgagg aacatcgccg tgaccatgcg caggcggttg tgcatccagc cggtcgccgc
     5041 gagctggcgc atggccgcat ccaccagcgg ataacccgtg cggccctcgc accagcgcgt
     5101 gaaatccacg ccgggttcgc gccaggcgat cgcctcggtc tcgggtcgga acgggcgggc
     5161 gcggctgacg cgggggaact ccgccaggat gtgggtgtag aactcgcgcc agaccagttc
     5221 gctcagccag gtctgcgcgc cgcgcgagcc gctgtcccat tcgccgtggt tgtgcgccag
     5281 cgcggcttcg atgcagcggc gcaccgagag cgcgccgatc gccagatacg gcgacagacg
     5341 cgaggtgccg tccagcgccg gcacgtcccg gtcctcgtgg tagcgcgcga ggccgtgctc
     5401 cacgaagtcc gccagccgcg cgcgcgcggc gacctcgccg atcggccacc agtcgggatc
     5461 cagcgtcgcc acgggcggct cccagtccgg cagcggcggg ggctcgaccg ccgcgcgcgc
     5521 cgtcggcgcc gcgacgcagt ccaccggctc accgcgcacc tgcgccagcc aggcgcggcg
     5581 gaacgggctg aacaccttga acggcgcgcc ctgctgcgtg cgcacggtgc cgggcgccag
     5641 caccgtgaag tcgtgcaggt tgcgccacgc ggtgccctgc gcgcgcaggg cctcggcaac
     5701 caggcggtcg cgcgccacct cgttgaccgc gtattccgcg ttggcataca ccgcggtcgc
     5761 agcctgcgcg cgcgcgaact ccgccaccgc tgccggagca ccggcgaact ccggtaccag
     5821 gcgcacccag agcggtatgc cgagcgccgc gagcctctcg cgcagcgcca gcacgtgccg
     5881 ccacacgaac acctgcttgc gcgcgcccca gtcgtgcgcg cgccactggg ccggcgtgag
     5941 caggaacagt ccggtcacgc gggcgccgtc gcgggcggcc tcggccagcg cgctgttgtc
     6001 gtcggcgcgc aggtcggagc ggaaccagac caccgtgctc atgcggctgg cggccggtac
     6061 tgtggcttgg cgagcaggaa ctgcgcggtg ccgatcacgc gttcgcggaa tccgccctcg
     6121 cagtagcaga agtagtagtc ccacagacgc acgaagtcct cgctgtagcc gagcgcgcgg
     6181 atggcctcgg tgcccgcgtg gaagcgccgg cgccaggcgg ccagcgtgtc ggcgtagtgc
     6241 gccgtgatgt cgagcgtgtc gatcaactgc atgtcggtgt gcgtccccgt gagcgtcgcg
     6301 atggcgccgg tcgagggcag gaagccgccc gggaagatgt agcgctggat gaagtccaca
     6361 ttgtggcgcg agcgcgcgta gcgctggtcc ggcatcagga tcgattgcag cagcatcagg
     6421 ccctcgggct tgagcaggcg gttgcaggcc gcgaagtact gcggcaggta gcggtggccc
     6481 accgcctcga tcatctcgat cgataccagc ttgtcgtact ggccctcgag ctcgcggtag
     6541 tcctgcagca gcacggtcac gcgctccgag agcccggccg cggcgatgcg ctgcgtggcg
     6601 tagtcgtact gctggcgcga gatcgtggtg gtggtcacgc ggcagccggt gtggcgcgcc
     6661 gcgtattccg ccatgccgcc ccagccggtg ccgatctcga gcaggtggtc atcgggcccc
     6721 agctccagcc ggcggcagat gcgcgcgagc ttggcgcgcg aggcctcctc gagcgtggcc
     6781 ccggcgtgcg ggaagatcgc ggaggaatac atcatggtcg ggtcgaggaa cagcgcgaac
     6841 atctcgttgc cgagatcgta gtgcgcctcg atgttgcgcc gcgagccggc cttgctgttg
     6901 cggttgagca ggtgcagcgc gcgcaacgcc acgcgctcga gcgccgagct gcgatcatcg
     6961 atggcgttca gcaggtcgat gttcgccgcc atcagctgca tcaccttcag caggtccggc
     7021 gtcttccagt gcccgcgtac ccaggactcg gccgcgccca cgctgccgcc gctggccagc
     7081 tcgtggtaga aggcgctgtc ggtgacctgc acgtgcgcca ccagttcctc gcgcgcgctc
     7141 gcgctgccga agcgccaggt gccctcggcg tcctcgatca ccagccgccc gcgtggcagc
     7201 gccgcgatgc gctcgaagat cttgccgcgc gcgatacgcg cggaccaggg caggcgcgcc
     7261 gtttgcggca gttccagttc gcgtgttctc gcggtgctca tcctgtggac tccttgatcg
     7321 gcatcgatgc gggtgggtgg gaatggaagg gcacgcgctt ggcgaacagg cgcagcgcct
     7381 cgaaatagat cgccgcgagg atgcgcaggc tcatcagcgg gtaacgcacc agcagcagcc
     7441 ggcgcagcgc gcgggcatcg agcggctcgc gctgcaggct gagcgtggcg tcgaagactt
     7501 tcgcgccgtc gcggtggttt tccaggtgga tgccgatgcg ctcgccgggg gtacggctca
     7561 tccagcggta acgcatgtcc atcgggttga acggcgagac gtggaaactc ttggcgaatt
     7621 ccgcgcgcag caccccggcg ttgtcctcga cgggcagcac gtaggcgtgg cgttctcccc
     7681 atggcgtgtt cgtgacctcg gcgaccacgg cctgcagcgt ctcgccgtcg gcgccgtagc
     7741 agtaataaca ggagatcggg ttcatcagca cgccgaagta gcgcaggttc gccagcagca
     7801 tcaccgggcc ggcggggcgg cggcccgtgt gcgcggcaac cagctcgcgc acgcactcgg
     7861 ccagcggccg cttcgggtcg ccgaggaaat ccttgcgccg gaaccagccc ggggccggac
     7921 gcgcggcgga ccacagccgg ctgccggcga acagtgtcgg cagcttctcg acgtcgagca
     7981 gcagcatgca catccggtag cggaaagcgt gcgtcgccgg cgtgaagcgc cggtggcgga
     8041 cctcgccggt gtagatcgcg ctcgtgctca ccatggcgcg cccagtgcct cggcgacgcg
     8101 caacgcgctc gccacgccgt cctcgtggaa accgttggcc caccacgcac cgcagaacca
     8161 ggtgcggcgc acgccgttga tctcggccca gcgctgctgg gcgcgcacgc cctcgacgct
     8221 gaataccggg tggctgtagc ggtagcgccc gaggatgcgc gacggatcga tggtggcggt
     8281 gtcgttcagc gtcacgcaga aggtgtggcg tgactccagc cgctgcagga tgttcatgtc
     8341 gtagctgagc acgggcagtt cgtcggcccc gggacgcagg cggtagttcc agctcgacca
     8401 cgcgcgcggc gcgcgcggca acacgcgggt atccgtgtgc agcgccacgt cgttgtcctg
     8461 gtaggggatc gccgacagga tgccgctctc ctctgcggag gggtcgccga gcagcgcgag
     8521 cgcctggtcg ctgtggcagc cgagcaccac gtggtcgaag cgctcgctga accccgcgcg
     8581 cgtacgcacg ctgacgccgt cggcctcgcg ggtgatgcgc tcgaccgggt caccggtgcg
     8641 gatcgcaccg gcgaagcggc gcgtcagcgg cccgaggtac tcgcgcgaac cgccgcggat
     8701 ggtgcgccat tgcggctggt cggtgacggt cagcaggccg tggttgcgaa agaaacgcac
     8761 gaagaactcg accgggaact cggccatgcc gcgcgtgccg gaggaccaga tcgccgcgcc
     8821 catcgggacc aggtaccagt cgcggaactc gcgcgagtag ccgttgcgcg ccaggtagtc
     8881 gccgagcagc atgccctcgg ggatgcggcc ctgctccaga tcggcagcgg cgctacggtt
     8941 gaagcgcagg atgtcgcgca acatgcgcca gaacgccggg cgcagcaggt tgccgcgctg
     9001 cgcgaacagg ctgttgaggt tgctgccggc atattcgatg ccctcgagct cgctgctgac
     9061 gctgaagctc atcgcggtag gctgcgaggc gacgccgagc tcgtccatca ggcggatgaa
     9121 gttcgggtag gtgcgatcgt tgaagacgat gaagccggtg tcgatggcat gacgctcccc
     9181 gtcgaggctg acatcgatgg tagcagtgtg gccgccgatg cgctcttcgc cctcgaacac
     9241 ggtcacctcg tgccgccggc tcagcagcca tgccgccgag agccccgcga tgcccgcccc
     9301 tacgatagct atcttcaccc gcgctcccgc catgaccctt gttgccgcga gtctacgctg
     9361 gcggggcggc ggtggatcaa ccccgtagcg ccggcttcgt gggtccggct tcagccggac
     9421 actgtcccga tgagtgccga cccacaggtg atccgccccg gtaccgctgg cgtaaacggg
     9481 ctcaacaact agccatcgaa cggagaaaag gtcgatgtca gcatccacgg tcctgcgctt
     9541 cccggggggg catggtttac atgcccggtc ccagccccga tcattacccc ccatgaacat
     9601 atccgcagtt ccggtggacc gggaacccga cgcatggacc acgcgcctgg aggcagtggc
     9661 gcgcgagcgc gaccggcagg cctatgcgga gcttttccgc cactttgcgc cgctgctgaa
     9721 atcctttgcg ctctccagcc ccggcatgtc ctccaacgcg cttgccgagg aactggtgca
     9781 ggaggtcatg ctcaagatct ggaaccgggc ctccaccttc gacgccggca aggccagtgc
     9841 gaccacctgg atctacaccc tcgcccggaa ctgccgcatc gacctgctgc gcaagaagtt
     9901 cagggtgcgc gaggaagtcg atgtcgatga actgtgggac ctgggcaccg aagacgatgt
     9961 gctggaggac gaggtctacc agaagcgcat cgaggtcgat gtgcgcgagt cgctcggcgg
    10021 gctgccggcc gaacagcgcc agatcatcac caaggtctac atggagggca agtcgcacag
    10081 cgaagtggcc gaggagctcg ggctgccgct ggggaccgtg aagtcgcggg tccgcctcgc
    10141 gatgagcaaa ttgaaagtga tgctggaccg atgagcgctg caaccatgac gcattaccat
    10201 cccgaccaat tcctgttgaa cgattacgtg gccggcagcc tgccgggcgc ggtggcgctg
    10261 ccgatcgcgg tgcacctgga gtactgcgcg cagtgccgcg aggaagtcgc gcagctcagc
    10321 cgtctcggcg ccgagctgtt cagcgagctc gatcccgtac ccgtgggcga cgatgccttc
    10381 gcgcggctgc tggatcaact ggacggcaag ggcgccgtgc cggtgacgcc gtcagcgccg
    10441 cggcgcccgg cgcgcgagcg cgatggcctg ccgcgtgcgc tgcgcagcct ggcgcccgcg
    10501 ggcgtggagg agctcgactg gaagcgcatc agcacctcgc tgcgcgcctc gcgcctgggc
    10561 ttcggcgacc cgcagcgcga ggtcgcgctg caccacatcc gcgccggcgg caaggtgctg
    10621 gcacacgggc acgccggcag cgagatcacg gtggtgctgc aggggagttt ctcggaccag
    10681 gacggccgtt atgcgcaggg ggatttcctg gtgcgcgggc cgggcgacga gcaccgtccc
    10741 gttgccgccg ccgactccga ttgcctgtgc ctggcggtgc tggatgcgcc gatccgcctc
    10801 ggcgggctgc tcgggcgcct cgcgaatcct ttcctgcgca tacatccacg ctgagcggga
    10861 ggggagatga gcgacgacgc ccggacagac ggcatcggaa tggagaagga cgccgacgca
    10921 gggcagtcgc agcaggagcc cggcgcggca ccatccagcg cgccgcagcc cgagcgtcgc
    10981 gacagtgaca agcgccttgt gctgatcgtc agtcacctgc tggtcgccat gctggtactc
    11041 tcgatgtggg cggcggcgga tacctggcag gcggtgaccg gcttggcgct ggccagtctg
    11101 ctctcggtgc tgacggcgtt cctcgccggc ttcgtgctcg gcaccctggt gcacgagtgg
    11161 gggcatttcc tcggcgctcg ccgcgcgggc gcgagctaca ccattcccgg gaagccggga
    11221 ctcttcgtgt tcaatttcga tttcgcccgc aacagcgcga gccagttcct gaccatgtcc
    11281 tatggcggtc agctgggcgg cgcgctggcg gtgctgctgc tctggcttgc ggtgccgacg
    11341 gacaccgcgg ggcgcgcgat gctcgttgcg gcaaccggcg gcggggcagt cttcgcggcg
    11401 gcgatcgagt ggccggtcat ccgtgcggtg cgcgcgggag gcgaacccgc cgcgcagctt
    11461 ggcaagatcg atcgcgcagt actgagcagc agtgcgctga aggggctcgt cggcaccgcg
    11521 ctgctctggc tgatcctgct ctagcgaatg gcggcatgtg cctcgctccc ggggcttgct
    11581 gcgcggccgt ggtgctccgt cgcccggccg cggcgggtcc aggcatgctg cgggacacgc
    11641 cgtgaatacg tccctgtagg ctcgactgcg gcatccatgc cgcagacggt cccgcatcat
    11701 ccctggcccc gccgcgacct gcgctggcaa tccgtcggcg ctgctcgcgc aggcatctag
    11761 agcgccggac tgtccggaat cagtcccagc atgatctgcg cggcgtcggt gtaggtcttc
    11821 tcgccgatga aggtgtgctg cgtgccggtg tagatgtcgc ggaaggcacg ctccaggcgg
    11881 ttgccctcgc ggatggccgt ggtgccggcg gcgaggtgag cccattgcac gacttcacgg
    11941 caggcctcgg tggcataggt cgcggcgagg cgcatctcgg cgcgcatcgc cggcgtgacc
    12001 gcggcgccgg cggcgacgga ggtctcgaaa ccctcgaagg tgtcagttat caacagccgc
    12061 gcggcacgcc acatgccctc gtggtgcgcg aggccgcgct ggaacgtggg cctgtgcgcg
    12121 agcgtgctca tgtcgcccat gcgcaccttc tccagtgcga gcgtcttcac atcgtcgagc
    12181 gcgctgcgcg ccacacccag cgcccaggcc gcgtggccgg ccgcggtgat cggcatgatg
    12241 ccgaaacggt acaggggccc gccacgcagc ggcgcgcgcg tgaacagcgg gaacaggcgc
    12301 gcctcgggca cgaacagctc ctgcacgttg tagtcgtagc tgcccgtcgc tttcagcccc
    12361 tgcacgaacc agccgtcggt gaagttcacc tgttcgcgcg gcatcaccgc gaccagcatc
    12421 tcgggcatgc ccagctcgcc caactgcatc tcgctgccgt gcatcgggat gaaaccgccg
    12481 accacgaagt tcgagtgccc ggtgccgctg ccgaaattcc agctgccgct caggcggtag
    12541 ccgccctcga cgcgctggcc gagcccgttc ggcgcgaact gcccgccgac cgtgctgcgg
    12601 ttgccgtgct gcacgaagac ctcgtcgtag ccggcctcgg gcaggtaggc ggcggcgaag
    12661 gcggcgccgg gaaagttcgc gatgccgatc cagcccacgg aaccatcctg ccacgcgagc
    12721 tcctgccagg tgtcgatcag ctcgcgaaac gagggttcgt gcccgccggc gcaatgcggg
    12781 ttcataaact gcatcagtcc gctgttccac agcgcctcga ccgtctcggc attcagcgtg
    12841 cgcgcctgtt cgttggcacc ggcattgcgc atcacggtct cgcgcacgga tcgggccatc
    12901 gcggggccgt cggttcgggc tgctgcgctc atgttcacct cgggtgtgtc gttctcgctg
    12961 cgccggcgag tgtcgcagaa gccggcacgg caccgcacgt tacgcggcgc taatgttctg
    13021 tgggttcggc ttcagcgggt gtcgcaagag ccatcaggtt gcgagagagc cgcttggtgc
    13081 gtcgccggct gtgctgcccg ccattgtcct gcggggcacg cacgagtac
//
LOCUS       DAORUQ010000090        13046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4735_length_13046_cov_7.040720, whole genome shotgun sequence.
ACCESSION   DAORUQ010000090 DAORUQ010000000
VERSION     DAORUQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4735_length_13046_cov_7.040720"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLW13_09260"
     CDS             <1..504
                     /locus_tag="PLW13_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQY70604.1"
                     /translation="HDPGHAVNAGEMLLEIASGDALVALGSAPPRLAGQIASGMRATT
                     ASGIEGEVTAVSGALDPATHALAVRFRLPGGAAAPGQMIELAIARPAPSGAVEVPAVA
                     VVDLAGTPTVFVARGDGFAAQPVDLLARDGASAWMTGVRADERVVTRGVLALKSLAES
                     DTDAGGE"
     gene            506..3583
                     /locus_tag="PLW13_09265"
     CDS             506..3583
                     /locus_tag="PLW13_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQY70605.1"
                     /translation="MLARLVEFALSQRLLMALLAVATIVGGGIAWRELPIDAFPDISP
                     TQVKLILKAPGMTPAEVEQRVIVPLEMELLGIPRQAMLRSSVKYAIADVTLDFAAGTD
                     IYWARQQVAERFAAVQADLPAGIEGGLAPIATPLSDLYMFTIEGEGYSLQERRTLLDW
                     VIRPALRTVPGVADVNALGGQVRTFEVVPDRIALTEAGLGIAELRAALERNNGNDGAG
                     RLDEGEEALVVRVTGALRGLDDIAATVVAMRADRVLRVGDLAEVREGSLTRYGAVTLD
                     GREETVQGLVVGLRGANAAQVVAGVKARLEVLGTSLPPGVRIEPFYDRSDLIQRATGT
                     VTRALFEATVLVVVLLMVFLGNLRAALVVAVTLPLAVLLTFVVMRLTGLTANLMSLGG
                     LAIALGMLVDASVVVVENAVEHLAEPEQSGAPPRLHRIWRAAAEVAAPVASGVAIIAL
                     VFMPLLTLQGLEGKLFAPVALTIVFALAGSLLLSFTVIPVLSSWLLQAGHHAEPWLMR
                     RLRGPYARALDFVLSRPRHASMIAATAFALALAAWFATGKTFMPTMDEGTVLVQLEKL
                     PSANIAASAAVDLAVQRAILARVPEVRSVIARVGADELGFDPMGLNQTDSFLVLAPKS
                     EWRHPDKEWLTGELRAVLEDFPGTAYAFTQPIEMRVSEMLTGARGDVAIKVFGPDLDV
                     LETLSADIERVVGGIPGAQDVYRLTNEGVQYLEIAVDRLAAGRMGFDVAGMQEELRAQ
                     VEGQVIGNVIENGRRVPLAIRGDESLRTDPARFADLPLAAPAGGTVRVGELASVARVS
                     GPVKIDRENGSRYALVQSNVAGRDLVGFVEDARAAVAAGVELPPGYTVSWGGQFENQQ
                     RAAARLALVVPVALGLIFLVLFTSFGSVRQAMLVLANIPFALVGGVLALWLSGEYMSV
                     PASVGFIALLGIAVLNGTVMVSHFNDLLARGRSVADTVVEGAQRRLRPVLMTASITAL
                     GLVPLLLASGPGSEIQRPLAVVVVGGLLSATPFTLLFLPLIFRRFGHATPGRN"
     gene            3583..3897
                     /locus_tag="PLW13_09270"
     CDS             3583..3897
                     /locus_tag="PLW13_09270"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HQY70606.1"
                     /translation="MDTALVQLALAFPVTVEEQLLAELRTIDPDLPGFTTLRGQGHGH
                     GYTRASVREQVRGRTDRGVLLMVLAPERATLIVEALRERLPLPEIAWWIVPVLGFGRL
                     GR"
     gene            3894..5177
                     /locus_tag="PLW13_09275"
     CDS             3894..5177
                     /locus_tag="PLW13_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY70607.1"
                     /translation="MNRLLRKAVLAVVAGICADAALAGEAALPEALRAALPSREAAVA
                     AIDGDTLVLRASAERAAAAARAQGYERGPHDWVGGAGYASRDVAQDQRYDEWELSVQR
                     GIRLPAKADADRRLARMEQEAATFAFADARHAAAVALLEAWIAWRGAMDQERITREAA
                     AMAVEDLRIVELRVRNGDAAAADLDAAQAAQAQAASSTRMAALAVEEARITLDVRFPG
                     LALPASPAALPEPAAPTVALPAWAERVVEHNHEIELAQARADLAALQAQRARLDRRGD
                     PVVGIRALSERGGDEQAVGVFVNVPLGSGARRFAAAEQADLANAAATVAVQVRREVQR
                     DAQVLLARAQGSLEAWQSAAAATRAAASHRERLQRGLALGGVRVSELLEARRRLLEAE
                     RAELAARATALTAIARLLLDAHAYWIDDGDHAEEP"
     gene            5298..6155
                     /gene="pbpG"
                     /locus_tag="PLW13_09280"
     CDS             5298..6155
                     /gene="pbpG"
                     /locus_tag="PLW13_09280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HQY70608.1"
                     /translation="MALLAGLMYSLLACAAALAEPRALDPHRLQLQSTKAVVFDARTG
                     QILYAKNAEATAPIASITKLMTAMVVLDAKLPMDEMLTISKDDVDRLKGTYSRLQIGT
                     RQSRKEMLRLALMSSENRAASALGRHYPGGRAAFIAQMNRKAAALRMSHTHFDDTTGL
                     SSRNLSSAGDLVKMTQAASQYALIREFTTTPERTVSFPQPRYSLGFVNTNRLVRAGSW
                     DIELSKTGYTNEAGRCLVMKTRVGDRPLVMVLLNSQGKLTPVGDATRIKQWLETSNYL
                     VLARRTTSA"
     gene            complement(6230..7978)
                     /locus_tag="PLW13_09285"
     CDS             complement(6230..7978)
                     /locus_tag="PLW13_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY70609.1"
                     /translation="MSKGTLARLLALATRDRRAFATAAALVLVAAAAEVGGPLLIKVF
                     IDEHVQTGSYPLATVLGLAGAYVVLQLIAAAAGYAQGVQLARIALAAVASLRERAFAT
                     ALRLPVAWFDRTPVGAVVSRLTNDTEAVKDFYVNVLGVVVANSARVIGMAVAMLLLDW
                     RLAIPCLAFLPAALAVMWAYQRVSGARFRAVRQALASINGALSESIGGVRAIQLTRQA
                     GRFDARFGELCSTHYRARMASLRLDAMMLRPLADVLMACSMAALVLWSGTEAMGGGIE
                     IGVIYVFVNYLGRLAEPVIDVTHRLSMFQSAMVSGARVFELSERSDARAPRLQGPLPA
                     DTRLRAEGVGFAYDAGPAVLHDIGFDIAPGRSLALVGATGSGKSTIAGLLLRFHEPAA
                     GTITLGGVPLADIDDGTFGRLVGYVPQDPFLLAGTIAENIDFGLGAGGARILEVAQRA
                     GLGPFLASLEPGLQTPVGERGAALSAGQRQQVILARALLRDPAILILDEATASIDSAT
                     EELLQQALHALRGKVSMVVIAHRLSTLREVDEIVVLARGHIEERGSHAQLIARGGLYR
                     RLWELQRMESPVDLSV"
     gene            complement(7975..9720)
                     /locus_tag="PLW13_09290"
     CDS             complement(7975..9720)
                     /locus_tag="PLW13_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY70610.1"
                     /translation="MNIFWELRGFFRAHWHRYAVAGLMLTAVALLGLLPPAIVGRVVD
                     GIHAQTLDAGELWLYAGAIVAAALGIYGLRFLWRQILYGASYSLGRELRRQIHAHLLG
                     LSPAVLGDFPAGDLMARATNDVQAVEMTAGEAVLSIFDGVMTGVLVLGVMVFTLSGWL
                     TLMALAPWPLMSWAMWRLGQQLHQHFDAAQGAFSRLNAVTQESVAGLRALRGLGAEAH
                     AEALLARASDEATEANLRVARVDSRYDPIIYLTVGASFLISIGGGAWLISLGRLTIGE
                     LTTFSLYLGQLVWPMFAFGWVANLVQRGKAAWERIQAFLRTPSAVPDSGTLAVVEDHR
                     LEVRVRSFSYPGRTRPALADLAFTLAPGRTLGIVGPTGSGKSTLLALLARFYDAPGVR
                     IRLGGRELAAYRLEALREAVALVMQESMLFSDTLRANLVLSRPAADDAAVMAAVRMAA
                     LDGDLAQLPAGLATEVGERGVTLSGGQRQRLCLARALLGEARILLLDDALSAVDAETE
                     HRILGELERHTRHLSRIVVSHRLSALQSAEEILVLREGCVAERGTHAALLAAGGWYAQ
                     TWRYQQLAATVEQRA"
     gene            9843..10463
                     /locus_tag="PLW13_09295"
     CDS             9843..10463
                     /locus_tag="PLW13_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY70611.1"
                     /translation="MTAYEHLRPAPASAASEGNATGRAVTRIEDVPDETLVAALARGD
                     QRALAQLVQRHGGWAARFAERMTGSPHVAEDVVQNAFLRLWNSAERWEGRSRFTTWFY
                     RVLHNLAVDELRRRRSGHEELDEALEDPAATPPQQLEQERRAARVRAALERLPERQRA
                     ALVLRHYEGCSQGEAAQILGISEGALESLLSRGRATLREQLRGELH"
     gene            10476..10907
                     /locus_tag="PLW13_09300"
     CDS             10476..10907
                     /locus_tag="PLW13_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70612.1"
                     /translation="MDIHRFSELAGAHGAARERWPEPDRALYDRFAASPEGLGILADA
                     GRIDAFLDGWQAPADDLGRVARIVAAANTPAHGARDTAPVPAARTGSPSRRIAAWLST
                     GFAASALCGFLLGFTQASANSDEEVYMDLLLGSNTVMEEFL"
     gene            10913..11398
                     /locus_tag="PLW13_09305"
     CDS             10913..11398
                     /locus_tag="PLW13_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQY70613.1"
                     /translation="MQTLKRPVVLGTLLAVSAAGNLFLGGILAGRIGGQAMHPPRYER
                     DFESRLRAVPEERRREIRDLLRQGKPAMRAQHEAMRELHEALATELTRDHPDRALLEK
                     QMADMRTRNLAMQEALHKSFLDTALTLAPEERRKMLDAMQEQRERGWRRGQADRHDAR
                     P"
     gene            11382..12422
                     /locus_tag="PLW13_09310"
     CDS             11382..12422
                     /locus_tag="PLW13_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY70614.1"
                     /translation="MTPDHDSSAPAAQPTVPLELVRAHLVAAAAAGLDLDALATRAGC
                     AGVFARPDEHRIPIDAFARLIRLTWDTLGSETAGFTEQPQRPGLFAMMCHATITCPNL
                     RRALLRAARFYALVQDEIAIELVEHGDEAQLRFAHRNRRGLDDRAFIESLMVIWVRWS
                     SWLIQRELLPDRIEFAFAPGSYREEYARMFPCDHYFGARENCVVFPARYLDHPVTRGS
                     AELAEFLNSAPESLMRRYIETPTLSSTVRRMLRRRDDIAGAGVEEIAALLGISAQTLR
                     RRLREEGNGFQEIKDSVRKESAIDQLLNSARTANEIAESIGFSEPSAFHRAFRKWTGT
                     TPGAYRDAALRR"
     gene            12553..>13046
                     /locus_tag="PLW13_09315"
     CDS             12553..>13046
                     /locus_tag="PLW13_09315"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQY70615.1"
                     /translation="MTDSRLFPHALDTLLALILCCLMPHGAIAAAPATDARAANVDLI
                     REQLAEHVGTLAYVWGYPMVDMATQMHNETHRVAPDQAVLAPRNAFHRREYLVTPASA
                     GNLRAPNNDTLYLSGWFDLAKEPVIVHVPDTAGRYYTLAVTDFFNEVTHIGRRTTGTA
                     EGYFA"
BASE COUNT         1985 a         4685 c         4576 g         1800 t
ORIGIN      
        1 cacgatcccg gccatgccgt gaacgccggc gagatgctgc tcgagattgc ctcgggcgat
       61 gcgctggtcg cgctcggcag cgcacccccg cgactcgccg gacagatcgc ttccggcatg
      121 cgcgcgacca ccgcgtccgg catcgagggc gaagtcacgg cggtcagcgg cgcgctcgat
      181 cccgcgacac acgctctggc ggtgcgtttc aggctgccgg gcggcgcggc cgcgccggga
      241 cagatgatcg agctcgcgat cgcgcggccg gctccgagcg gcgcggtcga ggtcccggca
      301 gtcgcggtcg tggacctggc cggcacgccc acggtgttcg tcgcgcgcgg cgatggtttc
      361 gcggcgcagc ccgtggacct gctggcgcgt gacggtgcct cggcctggat gaccggtgtg
      421 cgcgccgacg agcgcgtggt gacacgcggc gtgctggcgc tgaaatcgct ggccgaaagc
      481 gataccgatg cgggaggcga atgagatgct ggcccgcctg gtcgaattcg cgctgtcgca
      541 gcgcctgctg atggcgctgc tcgcggtcgc gacgatcgtc ggcggcggta tcgcctggcg
      601 cgaactgccg atcgacgcct tccccgacat ttccccgacc caggtcaagc tgatcctgaa
      661 ggcccccggc atgacgccag cggaggtcga gcagcgcgtg atcgtaccgc tcgagatgga
      721 actgctgggc atcccccgcc aggcgatgct gcgctcctcg gtcaagtacg caatcgccga
      781 cgtcacgctc gactttgccg cgggcaccga catctactgg gcgcgccagc aggtcgcgga
      841 acgtttcgcg gcggtgcagg ccgacctgcc cgccggcatc gagggcggac tcgcgccgat
      901 cgcgacccca ctttccgatc tctacatgtt caccatcgag ggcgagggct acagcctgca
      961 ggagcggcga acgctgctcg actgggtgat ccgcccggcg ctgcgcacgg tgccgggggt
     1021 cgccgacgtc aatgcgctcg gcggccaggt gcgcacgttc gaggtcgtgc ccgaccggat
     1081 cgccctgacc gaggccggcc tcggcatcgc ggaactgcgt gcggcgctgg agcgcaacaa
     1141 cggcaacgac ggtgcgggcc ggctggacga gggcgaggaa gcgctggtgg tgcgcgtgac
     1201 cggcgcgctg cgcggcctcg acgacatcgc cgcgacggtg gtcgcgatgc gcgccgaccg
     1261 ggtacttcga gtcggcgacc tggccgaagt gcgcgagggc tcactcaccc gctacggagc
     1321 agtcacgcta gatgggcgcg aggaaaccgt gcagggcctg gtagtggggc tgcgcggcgc
     1381 caatgccgcg caggtggtag ccggcgtgaa ggcgcggctc gaggtgctcg gcacaagcct
     1441 gcctcccggg gtgcgcatcg agcccttcta cgaccgcagc gacctgatcc agcgcgccac
     1501 cggcaccgtc acgcgcgcac tgttcgaggc caccgtgctg gtggtggtgt tgttgatggt
     1561 gttcctcggc aacctgcgcg cggcgctggt ggtcgcggtg acgctgccgc tcgcggtgct
     1621 gctcaccttc gtggtgatgc gcctgaccgg actcacggca aacctgatga gtctcggggg
     1681 gctggcgatc gcgctgggga tgctggtcga tgcctccgtg gtggtggtgg aaaacgccgt
     1741 ggagcacctg gccgagccgg agcagtccgg cgccccgccg cgactgcacc gcatctggcg
     1801 tgccgcggcc gaagtcgcgg caccggtggc ctcgggggtg gcgatcatcg cgctggtatt
     1861 catgcccctg ctgacccttc agggactgga aggcaagttg ttcgcgccgg tcgcgctcac
     1921 catcgtgttc gcgctggccg gctccctgct gctgtcgttc acggtgatcc cggtgctctc
     1981 gtcgtggctt ctccaggcgg gccaccacgc cgagccctgg ctgatgcgcc gcctgcgcgg
     2041 cccctacgcg cgcgcactgg acttcgttct gtcacggccg cgccacgcct cgatgatcgc
     2101 agccaccgcg ttcgcgctcg cgctcgccgc ctggttcgcc accggcaaga cgttcatgcc
     2161 cacgatggac gagggcacgg tgctggtgca gctggagaag ctgccctcgg ccaacatcgc
     2221 ggccagcgcc gcggtcgacc tcgcggtgca gcgcgccatc ctcgcgcggg tgcccgaggt
     2281 gcgctcggtg atcgcacgcg tcggtgccga cgagctcggc ttcgacccga tgggactcaa
     2341 ccagaccgac tccttcctgg tgctcgcgcc caagtccgag tggcgccacc ccgacaagga
     2401 gtggctcacc ggcgaactgc gcgccgtgct cgaggacttt cccggcaccg cctacgcgtt
     2461 cacgcagccg atcgagatgc gcgtttccga gatgctgacc ggcgcgcgcg gggacgtggc
     2521 gatcaaggtc ttcgggccgg acctggacgt gctcgagacg ctctcggccg acatcgagcg
     2581 cgtggtcggc ggcatcccgg gcgcacagga cgtctaccgc ctcaccaacg agggtgtgca
     2641 gtacctcgag atcgcggtcg accggctcgc cgccgggcgc atgggcttcg acgtcgccgg
     2701 catgcaggag gaactgcgcg cgcaggtgga aggccaggtg atcggcaacg tgatcgagaa
     2761 cggccggcgt gtcccgctcg cgatccgcgg cgacgagagc ctgcgtaccg atcccgcgcg
     2821 cttcgccgat ctgccgctcg cggcgccggc cggcggcacc gtgcgcgtcg gcgagctcgc
     2881 cagcgttgcg cgtgtcagcg gacccgtgaa gatcgatcgc gaaaacggct cgcgctacgc
     2941 gctggtgcag tcgaacgtgg cgggacgcga cctggtgggc ttcgtcgagg acgcgcgcgc
     3001 cgcggtcgcg gccggggtcg aactgccgcc gggctacacc gtgagctggg gtggccagtt
     3061 cgagaaccag caacgcgccg cggcccgcct cgcgctggtg gtaccggtcg cactggggtt
     3121 gatcttcctg gtgctgttca ccagtttcgg cagcgtgcgc caggccatgc tggtgctggc
     3181 gaacatcccc ttcgcgctgg tcggcggcgt gctcgcgctg tggctctcgg gcgagtacat
     3241 gtcggtgccg gcctcggtgg gcttcatcgc gctgctcggc atcgcggtgc tcaacggcac
     3301 ggtgatggtg agccacttca atgacctgct cgcccgcggc aggagcgtgg ccgataccgt
     3361 ggtcgagggc gcgcagcgac gactccggcc ggtgctgatg accgcctcca tcacggcgct
     3421 cggcctggtg ccgctgctgc tcgcgagcgg cccgggttcc gaaatccagc gcccgctggc
     3481 agtcgttgtg gtgggcggcc tgctgagcgc gaccccgttc acgctgctct tcctgcccct
     3541 gatattccgg cggttcggcc acgccacgcc ggggaggaac tgatggacac ggcactggta
     3601 caactcgcgc tggccttccc ggtgacggtg gaggagcagc tcctcgcgga gctgcgcacg
     3661 atcgacccgg acctgcccgg cttcaccacg ctgcgcggac agggccacgg tcacggctat
     3721 acccgcgcct cggtgcgcga gcaggtgcgc ggacgcaccg atcgcggcgt gctgctgatg
     3781 gtgctggcgc cggagcgcgc cacgttgatc gtcgaggcgc tgcgcgagcg cttgccgcta
     3841 ccggaaatcg cctggtggat cgtgccggtg ctgggcttcg ggaggctggg ccgatgaatc
     3901 gcctgctgag aaaagccgtg ctggcggtgg tggccgggat ttgcgccgac gccgcgctcg
     3961 cgggcgaagc ggcgctgccc gaggcgctgc gcgcggcatt gccgtcgcgc gaggcggcgg
     4021 tggcggccat cgatggcgac acgctggtgc tgcgggcgag cgcggaacgc gccgctgccg
     4081 cagcccgggc acaaggctac gaacgcgggc cgcacgactg ggtgggcggc gccggttatg
     4141 cctcgcgcga tgtggcgcag gaccagcgct acgacgagtg ggagctgagc gtgcagcgcg
     4201 gcatacgcct gcccgccaag gctgacgccg accgccgcct cgcccgtatg gagcaggagg
     4261 ccgcgacctt cgccttcgcc gacgcccggc acgcggccgc ggtcgcgctg ctggaggcgt
     4321 ggatagcctg gcgcggtgcc atggaccagg aaaggatcac gcgcgaggcg gccgcaatgg
     4381 cggtcgagga cctgcgcatc gtggagctgc gggtgcgcaa cggcgatgcc gcggccgccg
     4441 acctcgacgc ggcgcaggcc gcccaggcgc aggccgcaag ctcgacgcgc atggcagcgc
     4501 tcgcggtgga ggaagcgcgg atcacgctgg atgtccgctt ccccggcctg gcgctgccgg
     4561 cctcgcccgc ggcgctgccc gagccggcag ccccgaccgt tgcgctgccg gcctgggccg
     4621 agcgggtggt cgagcacaac cacgagatcg agctggcgca agcgcgggcc gatcttgccg
     4681 cactgcaggc gcagcgcgcg cggctggacc ggcgcggcga tccggtagtc ggcatcaggg
     4741 cgctgtcgga acgcggcggc gacgagcagg ccgtcggtgt tttcgtcaac gtgccgctag
     4801 gcagcggggc gcgccgtttc gccgcggccg aacaggcgga tctcgccaac gcggcggcga
     4861 cagtcgccgt gcaggtgcgg cgcgaagtgc agcgcgacgc acaggtcctg ctcgctcgcg
     4921 cccagggcag cctcgaggcc tggcagagtg ccgcggcggc cacccgcgcg gcggcgagcc
     4981 atcgcgaacg gcttcagcgc ggcctcgcgc tgggcggcgt aagggtcagc gaactgctgg
     5041 aggcgcggcg gcgcttgctc gaggccgaac gcgcggagct tgccgcgcgc gcgacggcac
     5101 tcaccgcaat cgcacgcctg ctgctcgatg cacatgccta ctggatagac gacggcgacc
     5161 acgcggagga gccctgagcg ccccatttgg ggttgcagcc atgatttcta ctggctgatc
     5221 cggaacttat ctattactct tcatgtgatc ggcgaacatt ggagagtatc ggcatgaagc
     5281 gcgggaaaag tacgtccatg gccctgctgg cggggctcat gtacagcctg ctcgcctgcg
     5341 ccgcggctct cgccgaacca cgggcgctgg acccgcaccg cctgcaactg caatcgacca
     5401 aggccgtggt gttcgacgcg cgcaccggtc agatccttta cgcaaagaac gccgaagcca
     5461 ccgcgccgat cgcctcgatc accaagctga tgaccgcgat ggtggtgctc gacgccaagc
     5521 tgccgatgga cgagatgctg accatctcga aggacgacgt cgatcggctc aaaggaacct
     5581 actcgcggct gcagataggt acccggcagt cgcgcaagga aatgctgcgc ctggcgctga
     5641 tgtcatccga gaaccgcgcg gcctcggcgc tgggccgcca ctacccgggt gggcgcgccg
     5701 ccttcatcgc gcagatgaac cgcaaggccg ccgcgctgcg catgagccac acccacttcg
     5761 acgacaccac cgggctgtcg agccgcaacc tctcctcggc cggcgacctc gtgaagatga
     5821 cgcaggccgc cagccagtac gcgctgatcc gcgagttcac gaccaccccg gagcgcacgg
     5881 tgagttttcc gcagccgcgc tattcgctcg gtttcgtgaa caccaatcgc ctggtgcgcg
     5941 cggggtcgtg ggacatcgag ctgagcaaga ccggatacac caacgaagcc ggccgctgcc
     6001 tggtcatgaa aacccgcgtc ggcgaccgtc cgctggtgat ggtgttgctg aattcgcagg
     6061 gcaagctcac tcccgtgggt gacgccacgc gcatcaagca gtggctcgaa accagcaatt
     6121 acctcgtcct cgcccgccgc accacgtccg cctgagcctc gccgtcggtc cggattcgtc
     6181 cggacaaatg tccgcaccgc gcaggtccgc attcatgagg acaggggctt cagaccgaga
     6241 gatccaccgg cgattccatg cgctggagct cccatagccg gcggtagagg ccgccgcgcg
     6301 cgatcagttg cgcatgggag cctcgctcct cgatgtggcc gcgcgcgagc accacgatct
     6361 cgtcgacttc gcgcagcgtc gagaggcggt gggcgatcac caccatcgac accttgcccc
     6421 gtagcgcatg cagcgcctgc tgcagcagtt cctcggtcgc gctgtcgatg ctcgccgtgg
     6481 cctcgtcgag gatcagtatc gcgggatcgc gcagtagcgc ccgcgccaga atcacctgct
     6541 ggcgctggcc ggcggacagc gctgcgcccc gctcgcccac cggtgtctgc aatccgggct
     6601 ccaggctggc gaggaaggga ccgaggccgg cgcgctgggc aacttccagg atccgcgccc
     6661 ccccggcacc cagcccgaag tcgatgttct ccgcgatcgt gcccgcgagc aggaagggat
     6721 cctgcggcac atagcccacc agccgcccga aagtaccgtc gtcgatgtcg gcgagcggca
     6781 ctccgcccag cgtgatcgtg cccgccgcag gttcatggaa acgcagcagc agccccgcga
     6841 tcgtgctctt gccgctgccg gtcgccccca ccagcgccag cgaccgcccg ggcgcgatgt
     6901 cgaagccgat atcgtgcagc accgccggcc cggcgtcgta ggcgaagccg acaccctcgg
     6961 cgcgcagccg cgtgtctgcc ggcagcggtc cctgcagccg tggcgcgcgc gcatcgctgc
     7021 gctcgctcag ctcgaacacc cgcgcgcccg agaccatcgc gctctggaac atgctgagcc
     7081 ggtgcgtgac gtcgatcacc ggctcggcaa gccgtccgag gtagttcacg aacacgtaaa
     7141 tcacgccgat ctcgatgccg ccccccatcg cctcggtgcc ggaccacagc accagcgcgg
     7201 ccatgctgca cgccatgagc acgtccgcca gcgggcgcag catcatcgcg tcgagccgca
     7261 acgaggccat gcgcgcgcgg tagtgcgtgc tgcacagctc gccgaagcgc gcgtcgaagc
     7321 gcccggcctg gcgcgtgagc tggatggcgc gcacgccgcc gatcgactcc gacagcgccc
     7381 cgttgatcga cgccagcgcc tggcgcaccg cgcgaaagcg cgcgccgctc acgcgctggt
     7441 aggcccacat caccgccagc gcggccggca ggaaggcgag acagggaatc gccaggcgcc
     7501 agtcgagcag cagcatcgcg accgccatgc cgatcacgcg cgcgctgttg gcaaccacca
     7561 cgcccagcac gttgacgtag aagtccttca ccgcctcggt gtcgttggtg agccgcgaca
     7621 ccaccgcgcc caccggcgtg cggtcgaacc aggccaccgg caggcgcagc gccgtggcga
     7681 aggcccgctc gcgcaggctc gcgaccgcgg cgagcgcgat gcgcgccagc tgaacgccct
     7741 gcgcgtagcc cgcggccgcg gcgatcagct gcagcacgac gtaggcaccc gccagtccca
     7801 gcaccgtcgc gagcgggtac gagcccgtct gcacgtgctc gtcgatgaac accttgatca
     7861 gcagcgggcc gcccacctcg gccgcggccg cgaccagtac cagcgcggcg gcagtcgcga
     7921 aggcgcgccg atcgcgcgtg gccagcgcga gcagccgcgc cagggtaccc ttgctcatgc
     7981 gcgctgctcc acggtggccg ccagctgctg gtagcgccag gtctgcgcgt accagccacc
     8041 ggccgcgagc agcgcggcgt gggtgccgcg ctcggcaacg cacccctcgc gcagcaccag
     8101 gatctcctcg gccgactgca gcgccgagag acgatggctg acgactatgc ggctgaggtg
     8161 acgcgtatgg cgttcgagct cgccgaggat gcggtgctcg gtttcggcgt ccacggccga
     8221 cagcgcgtcg tcgagcagca ggatcctcgc ctcgccgagc agcgcgcgcg ccaggcacaa
     8281 gcgctggcgc tgcccgcccg agagcgtgac cccgcgctcg cccacctcgg tggccagccc
     8341 cgcgggcagc tgcgcgaggt cgccgtccag cgcagccatg cgcaccgcgg ccatgaccgc
     8401 ggcgtcgtcc gccgcgggcc gcgacagcac caggttcgcg cgcagcgtgt cggagaacag
     8461 catcgattcc tgcatcacca gcgccacggc ctcgcgcagc gcctccagcc ggtacgccgc
     8521 gagctcgcgc ccgccgaggc ggatgcgcac gccgggcgcg tcgtagaaac gcgcgagcag
     8581 cgcgagcagc gtggacttgc cagagccggt cgggccgacg atgccgaggg tgcgtcccgg
     8641 cgccagcgtg aacgcgagat ccgccagcgc aggccgggtg cggcccgggt agctgaagga
     8701 ccggaccctc acctcgaggc ggtgatcctc gaccacggcg agggtgccgc tgtcgggcac
     8761 cgccgagggc gtgcgcagga acgcctggat gcgctcccag gcagccttgc cacgctgcac
     8821 caggttcgcc acccagccga aggcgaacat cggccacacc agttgcccca ggtacaggct
     8881 gaaggtggtg agctcaccga tcgtgagccg tcccaggctg atcagccagg caccgccgcc
     8941 gatgctgatc aggaacgagg cgcccacggt gaggtagatg atcgggtcgt agcgcgaatc
     9001 gacgcgcgcc acgcgcaggt tcgcctcggt cgcctcgtcc gaggcgcgcg ccagcagggc
     9061 ctcggcatgc gcctcggcgc ccagcccgcg cagcgcgcgc agcccggcca cgctctcctg
     9121 ggtcaccgcg ttcagccggc tgaaggcgcc ctgcgccgcg tcgaaatgct ggtgaagttg
     9181 ctgccccagg cgccacatcg cccagctcat cagcggccac ggtgccagcg ccatcagcgt
     9241 cagccagccg ctcagcgtga acaccatcac cccgagcacc agtacgccgg tcatgacgcc
     9301 atcgaaaatc gagagcaccg cctcgcccgc ggtcatctcc acggcctgga cgtcgttggt
     9361 cgcgcgcgcc atcaggtcac cggcaggaaa gtcgccgagc accgccggcg acagcccgag
     9421 caggtgagcg tggatctgcc ggcgcagctc gcgcccgagc gaataggacg cgccgtagag
     9481 gatctgccgc cacaggaagc gcaacccgta gatgcccagc gccgcagcca cgatcgcgcc
     9541 ggcgtacagc cacagctcgc cggcgtccag cgtctgcgcg tggataccat cgacgacgcg
     9601 gcccacgatg gccggtggca gcaggccgag caatgcgacc gcggtcagca tcaggcccgc
     9661 caccgcgtag cggtgccaat gcgcgcggaa gaagccgcgc agttcccaga agatgttcat
     9721 gtccgcacga tacccgatcg cgtcggagcg aaggaaagca cggcgcgtgt ccgtcaaaga
     9781 atgcacacga atggcccgcg ctccggttgg cgcggtggcc tggcaacggg gactttgcga
     9841 acatgacggc gtacgagcac ttgcgcccgg caccggcatc ggccgccagc gagggcaacg
     9901 ctacggggag agccgtgacg cgcatcgagg atgtcccgga cgaaaccctg gtcgcagcgc
     9961 tcgcgcgagg cgaccagcgc gcgctcgcgc aactggtgca gcgccacggc ggctgggccg
    10021 cgcgcttcgc cgagcgcatg acgggcagtc cgcatgtcgc cgaggacgtg gtgcagaacg
    10081 cgttcctgcg gctgtggaac agcgccgagc gctgggaagg ccgttcgcgg ttcacgacct
    10141 ggttctaccg cgtgctgcac aacctggcgg tggacgagct gcgccggcgc cgcagcggcc
    10201 acgaggagct ggacgaagcg ctcgaggatc ccgccgccac gccaccgcaa cagctcgagc
    10261 aggagcgccg cgccgcgcgc gtgcgcgccg ccctcgagcg cctgcccgag cgccagcgcg
    10321 cggcactggt gctgcgccac tacgagggct gctcgcaggg cgaggcggcg cagattctcg
    10381 gcatcagcga gggcgcgctg gaatcgctgc tctcgcgcgg gcgcgccacg ctgcgcgagc
    10441 aactgcgcgg cgaactgcac taggaggacg gacgcatgga catccaccgt ttcagcgaac
    10501 tggccggcgc ccacggcgcc gcgcgcgaac gctggcccga gcccgatcgc gcgctgtacg
    10561 accgtttcgc cgccagcccc gagggcctgg gaatcctggc cgatgccggg cgcatcgacg
    10621 ccttcctcga cggctggcag gcgccggccg acgacctcgg gcgcgtggcg cgcatcgtgg
    10681 cggcggcgaa cacgcccgcg cacggcgcgc gggataccgc gccggtgccc gctgcccgta
    10741 cggggagccc ctcgcgcagg atcgcggcgt ggctctcgac cggcttcgcg gcctcggcgc
    10801 tgtgcggctt cctgctcggc ttcacgcagg ccagcgcgaa cagtgacgag gaggtctaca
    10861 tggacctgct gctcggcagc aacaccgtga tggaggaatt cctgtgagcc tcatgcagac
    10921 cctgaagcgc ccggtcgtgc tgggcacgct gctcgcggtg tccgccgcgg gcaacctgtt
    10981 cctcggcggc atcctggccg ggcgcatcgg cgggcaagcg atgcacccgc cgcgctacga
    11041 gcgcgacttc gagtcgaggc tgcgtgccgt gcccgaggag cgccggcgcg agatccgcga
    11101 cctgctgcgc cagggcaagc ccgccatgcg cgcacagcac gaggcgatgc gcgaactgca
    11161 cgaggcgctc gccaccgagc tcacgcgcga tcatccggat cgcgcactgc tcgaaaagca
    11221 gatggccgac atgcgtacgc gcaacctcgc gatgcaggaa gcgctgcaca agagcttcct
    11281 ggataccgcc ctgacgctgg cgccggagga gcggcgcaag atgctcgacg ccatgcagga
    11341 acaacgcgaa cgcggctggc gccgcgggca ggccgaccgg catgacgcca gaccatgatt
    11401 cctccgcgcc ggcggcgcag ccgaccgtgc cgctcgaact ggtgcgcgcc cacctggtgg
    11461 ccgcggccgc ggcggggctg gacctcgacg cgctggctac ccgggccggc tgcgcaggcg
    11521 tgttcgcgcg ccccgacgag caccgcatcc cgatcgatgc cttcgcgcgc ctgatccgcc
    11581 tcacctggga cactctcggc tcggaaaccg ccggcttcac cgagcagccc cagcgaccgg
    11641 ggctgtttgc catgatgtgc cacgccacga tcacctgccc caacctgcgc cgggcgctgc
    11701 ttcgagccgc gcgcttctat gcgctggtgc aggacgagat tgccatcgag ctggtggagc
    11761 acggcgacga ggcgcagctg cgcttcgcgc accgcaaccg ccgcggactc gacgaccgcg
    11821 ccttcatcga atcgctgatg gtgatctggg tgcgctggtc gagctggctg atccagcgcg
    11881 aactgctgcc cgaccgcatc gagttcgcct tcgcgccagg cagctaccgc gaggagtacg
    11941 cgcgcatgtt cccctgcgac cattacttcg gggcgcgcga gaactgcgtg gtgttccccg
    12001 cgcgctatct cgaccacccg gtgacgcgcg gcagcgccga gctcgcggaa ttcctcaaca
    12061 gcgcgccgga gtcgctgatg cgccggtata tcgagacccc gacgctctcg agcaccgtgc
    12121 gccgcatgct gcgccggcgc gacgacatcg ccggcgcggg cgtcgaggag attgccgcgc
    12181 tgctcggcat cagcgcccag acgctgcgcc gccggctgcg cgaggaaggc aacggcttcc
    12241 aggagatcaa ggactcggtg cgcaaggaga gcgccatcga ccagctgctg aactccgctc
    12301 gcaccgcgaa cgagatcgcc gagtcgatcg gcttctcgga acccagcgcc tttcaccgcg
    12361 ccttcaggaa gtggaccggc accacgccgg gcgcgtaccg cgacgcggcg ctgcgccgct
    12421 agcacgcgtt ggcgcccacc atgggcagag gtttgccggc tgtgtacccg gcaggcccag
    12481 ccgcggttaa gctgcttcca tcatcgccgc gcccgcgggc cacgcatgcg ctcaaagcat
    12541 aaaggagaac aaatgaccga ctcacgcctg ttcccccacg ccctcgacac gcttctcgcg
    12601 ctgatactct gctgcctgat gccgcacggt gccatcgccg cagcccccgc cacggacgcg
    12661 cgtgccgcca acgtcgacct gatccgcgag cagctggccg agcacgtcgg cacgctggcc
    12721 tacgtctggg gctatccgat ggtcgacatg gcgacgcaga tgcacaacga gacgcaccgc
    12781 gtggcgccgg accaggcggt gctggcgccg cggaacgcgt tccatcgccg cgagtacctg
    12841 gtcacaccgg ccagcgccgg caacctgcgc gcgcccaaca acgacacgct gtacctgagc
    12901 ggctggttcg acctcgcgaa ggagccggtg atcgtgcacg tgccggacac cgccgggcgc
    12961 tactacacgc tggcggtcac cgatttcttc aacgaggtca cgcacatcgg ccggcgcacc
    13021 accggcaccg cggaaggcta tttcgc
//
LOCUS       DAORUQ010000091        13001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4768_length_13001_cov_5.655801, whole genome shotgun sequence.
ACCESSION   DAORUQ010000091 DAORUQ010000000
VERSION     DAORUQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_13001_cov_5.655801"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..788)
                     /locus_tag="PLW13_09320"
     CDS             complement(66..788)
                     /locus_tag="PLW13_09320"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70616.1"
                     /translation="MPPRPRSDPSAQTRKRILDTAERLFGERGVAAVSLRDINRTAGI
                     SQGVLHYHFGGRDALIEAILQRWLPGVNAERRQMYDAILAAGRTPSERDVVEILCLPL
                     ARLATARQPAGRRFLRCLARLGQEENPVWARTTRTQMSGVELHEALQACAPGASPALI
                     EWYFSMMLNVIHGTLSEAGRAARSWQRALAAEPMSPEDQAEALVEFICEGVGGLVRRN
                     QPAAGTPRAQESRPRGRGAARA"
     gene            complement(807..3089)
                     /locus_tag="PLW13_09325"
     CDS             complement(807..3089)
                     /locus_tag="PLW13_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY70617.1"
                     /translation="MRNTRGSLRLGATLFALSAGLGMVQYAAAAAPPAEAPVVTRQLA
                     PAGAPNIVVVLLDDVGFGAAGTFGGPVETPALDALAHDGLRYNRFHTTAICSPTRAAL
                     LTGRNSHAAGVGSVMNTATSYPGYRGVLREDTATIARILGQNGYATSAWGKWHLVPDW
                     EASQTGPFTRWPTGAGFDKFYGFLGGETDQYEPTLYDGTTPVVRERAPGYHLTEDMAE
                     QAVAWMQMQRAVDPARPFFVYFAPGAAHAPLQPPAAWLERYRGKFDQGWDRLREETFA
                     RQQAAGVIPRDAVLTPRPDALPAWDSLTPEEQRVSARLMELYAAFLAHTDAQVGRLRD
                     ALKDMDQYDNTLFVYIVGDNGASAEGGLKGSSNYMGDLQGLVSEVARFGDAPQTLSGE
                     NTYAHYNSGWAWGTNTPFQWTKQVASHLGGTRNPMVLAWPERIRDRGGLRSQFGHVND
                     IVPTILEVTGIAAPASVDGVAQRPMDGVSLAYSFDDAKAAERHATQYFEIFGNRSIYH
                     EGWMASAFRGRAPWQVNSAARDEDFDADRWELYDLRADFSQGTDLAAREPARLRELQQ
                     LFAQQADANDVLLHNPTPENMRFPSLAGDRTRFTFHAGTIAVAEKEAPNTKTRSHAIE
                     ARLRVPDGGASGVLATLGGRSAGWSLYLDQGGVPVYRLRIFDAEEITLRGTTALAPGE
                     HVLRYEFTTTGAGPVPGGKGRLLVDGTEVARGEVTRTATFFSIDETFDIGLDTGSSPA
                     DYRAPYPFTGSIGQVTIELL"
     gene            3320..5926
                     /locus_tag="PLW13_09330"
     CDS             3320..5926
                     /locus_tag="PLW13_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQY70618.1"
                     /translation="MSTRMYPDCAAPRKPLAAAVGWTMALACYAAMDAAPARAQGAAL
                     EEVIVTAQKRQENVQDIPATVNAVTASQIADFRVVSLDDVQALTPGVLMVRNDARRQS
                     VTIRGITADPDNNAAAPISYYWNDAPLRSQQAFTTFFDIERLEVLRGPQGTLQGRTDP
                     AGAILIHTKRPDLQAVSGTVRQSFSDNSGMQTEAGVSVPLVKDVFGVRLAGLWDESDG
                     SEYENIVNGQDESQQTGSYRISLDWMPLEALEISLVHERSDVDAEVPEGAQGDVGNVI
                     NAITAQRNGASFVFNQTLAGINNPNALAGAAFAAAPSAFIPGQTNAGALVQTNIARRA
                     VAPTWLAGVSLDDDDRQSIQLGRNDLDMRSEFTVLRAVWELENHTLTSIGSLKDWRQN
                     NWLDMNFAYLSPVAQMQNTHSEAEDWSQEIRFASNDNAFWNYTAGMFWEDFDGFTNNY
                     TDLGGPFAMYGSMAGITNGDMVQHLQIPNGRETLAFFLHNRLELSEATNLQLGIRWQE
                     IDGEQIVRAEVIDNYAPPAESGPVTNPLLAGALNPRLTSAGVWNIFKGNIRTGDLIPN
                     ELQTTEEREWTGGVKLSHYLNDDDMVYASLDRSFRPSGATITPTALSADSLLFDSETS
                     ASLELGYKAMAFDGNLRFSAAAYYQQYSDYLPRASNVNALVATGAGRVPQVVQGGLVF
                     NADATVMGVEAEWTALLGERFQFGGGLSYSEATFDDDEPGPCNRPFTPAEIADPTVEI
                     ATCDIGGEDVSSQPNWSATLNAEYTLPLSFGELFVRPLLAYTGEREDRLVPAQSFDDY
                     VLVNLYAGVRSEDGVWEGTVWAKNLFDEDANSVFSNPYFGPLGGNSNFTRAVMVPPRL
                     LGVTVSYNFAGG"
     gene            5926..7818
                     /locus_tag="PLW13_09335"
     CDS             5926..7818
                     /locus_tag="PLW13_09335"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY70619.1"
                     /translation="MGSMPRRLLAVPTLLAACSGAVCAGESYSPYAGDAHPARVYWGD
                     THLHTNMSADANIAGDMRMGPDEAYRFAKGETVRAQNGMSVRLQRPLDFLVVADHAEY
                     LGLTVGLLADDPQLLADPVGKRWHALLKANTATRIESAADATGYTKEMMVESFLPQRE
                     VLRDEAFAASAWKQVAANADRHYSPGTFTAFAGYEWSSMPEIDNLHRVVIFRDGFDKT
                     SQVRAFSAFDSQDPEQLWRYLEEYERMTGGAAIAIPHNSNASNGRMFELKDMQGRPLT
                     RDYAERRARWEPLLEVTQIKGDSEAHPLLSPDDAFADYETWDRGNLLFSKPKEPWMLQ
                     HEYARSALKLGLQQYAALGVNPFKFGMIGSTDAHTGLAAVEENNFWGKVIASEPSAER
                     WRKPFFKGEALEVRNSELAASGYAAVWATENTREAIFAAMKRRETYATTGPRMTVRFF
                     GGWEFVPGDEHAPDPARRGYAGGVPMGGDLPAPATAGAAPGFLVAALKDPEGANLDRI
                     QIVKGWHDADGELHEKVYDVALADGRGPGADAASIGSSVDVARASYSNSIGDAELAAV
                     WRDPGFDPAQSAFYYARVIEIPTPRWTARDAEYFGVGMDREVTMITQERAYTSPIWYT
                     PANERR"
     gene            7841..8605
                     /locus_tag="PLW13_09340"
     CDS             7841..8605
                     /locus_tag="PLW13_09340"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY70620.1"
                     /translation="MIGRVANKVAMVTGGASGLGEATSLLLAREGARVAVADVAQEAG
                     EHLAERIRAEVGDAMFVPLDVSSESAWDAAMAQVQQRCGKLNILVNNAGIAPEGDMEM
                     SFELWRKVMSVNLDGTFLGTRAGIRAMRSSGEPGSIINISSTMAMTAFPTTAAYSASK
                     GGVRSLTKAAAMYCGAHRLPIRVNSIHPGMCVTPLVAGYYENNPEALQAQIDMHPIGH
                     LGTAEDIAEGVLYLASEASRFVLGSELVIDGGFLAR"
     gene            8660..10162
                     /locus_tag="PLW13_09345"
     CDS             8660..10162
                     /locus_tag="PLW13_09345"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQY70621.1"
                     /translation="MNNSSVFRLLATACILGALAAPLRAGTAPSPIDPGLAPERVAAL
                     TEQAFYWGINIAGFYELRHLYTQLEGHPAFRGINRMQPHKQLFDASVRIATTVNASTL
                     YSGGAFDVSREPIVVEAGAVGDGRYWSVQAADQNANWFFMAGSQFSGNAAQRYLILGP
                     KWRGRLPAAFRSTQIVRASSDSFTLAVRVAVTTRDAQDMRAAGAVIDNVLVAPLSQWE
                     ANGGTLPPLERQPVVKGDYRFFPRMSAIGDIGKSMTPVDYLQLLSLAINDPALTKRTD
                     SAKELATLAALAPLGLREGLIFDPAGLTAEQQSAIARGFARGRANAKLALEKSLIDMN
                     GWKLQSSLFHDDLDYVAKAGANDVAWGTPVPYQSHSIAYVFRDSKGRALDGRHRYTLT
                     FDLENMPPVTEFWELPVYDEYGYFIANAIDRNSVTSYLHKAGAYAEGNGRLTFYLQPE
                     KPSDPEQARNWLPTSATGGFQLAARFYGPMAPLIDGSYAMPAIVRSDDAD"
     gene            10217..12571
                     /locus_tag="PLW13_09350"
     CDS             10217..12571
                     /locus_tag="PLW13_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY70622.1"
                     /translation="MTVARSTRIASRRSRLGGALLALALALAAAPALAQTVLPRPAEP
                     FTGKIGTTYADSTPAFPAPVTAPAGAPNVVLVLTDDVGFGAASTFGGAIPTPHLDRLA
                     EEGLRFNRFHTTAMCSPTRAALLTGRNHHAVGNGIVANLTTGFPGYNNLMPKSAATIA
                     EVLRQHGYNTAMFGKHHNAPEAHVSAAGPFDMWPTGLGFEYFFGFMAAETNQFTPALY
                     RGITPVPTLEEGVLDKALADDAIHWLHQQQAAAPGKPFFMYYATGTAHAPLQAPADWI
                     ARFEGKFAGGYDRMREEVVERQKRMGLVPADTVNTPRPEEIAAWDALTPEQKKINERF
                     MEVYAAMLAYQDEQVGRLFAEIGRMGLRDNTLVIFIEGDNGAAAEGGMHGSMNPMSNF
                     ANGTQESEATLLANLERIGGPEAVAQYGYGWTWATNAPFRLAKQYASHLGGTRNGMVV
                     SWPQRIKSSGIRPQFSHVTDIAPTIYEAAGVTAPDTVNGVEQQPIDGSSLLYAFDNAA
                     AAERHDTQYFEMMGNQAIYHQGWLASSTPAIAPWARDRRNDTLPSEYPWELYDLRADF
                     SQAHDLASAHPEKLAQMKALFLEEARRNQVLPLDNRLSMERFLAASSQQRKANTYTYW
                     GAGVSVPAVSAAPLLNRSFTISAEIDAPAPAQGVVLALGSRFGGWSFFVQDGTPVALM
                     AASQLEGDQSRVVASGRLPAGKSVLSYQFVSDGGVNAGGEMRISVDGREIGRGRIART
                     ISKLPEMTDTLDIGFDADTPVTAEYRDGGRFNGMLSRVDVSIAN"
     gene            12627..>13001
                     /locus_tag="PLW13_09355"
     CDS             12627..>13001
                     /locus_tag="PLW13_09355"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY70623.1"
                     /translation="MNTSKRRAGGISFGAWICFCVLLAPVPGAAAGDDGPRPGAAAEE
                     SGALASSRRRVLFGELHLHTSYSFDAFGFSPSRIDPDTAYRFARGEAVDYLGKPARRH
                     APLDFMAVTDHAEYMGFLNTIED"
BASE COUNT         2139 a         4375 c         4459 g         2028 t
ORIGIN      
        1 agtatcccgt agacggcggc tggaacgcgg ggttgcgcaa cgagcggttg ccgggctcct
       61 gagcctcagg cgcgcgcggc gccccggccg cgtggccggc tctcttgtgc gcgcggagtt
      121 cccgcagccg gctggttccg ccgcaccagg ccgccgacgc cctcgcagat gaactccacc
      181 agtgcctctg cctggtcctc ggggctcatc ggctccgcgg cgagcgcgcg ttgccaggag
      241 cgcgcggcgc gcccggcttc gctgagcgtg ccgtgaatca cgttcaacat catgctgaag
      301 taccactcga tgagcgcggg gctcgcgccg ggtgcgcagg cctgcagcgc ctcgtgcagt
      361 tccacaccgc tcatctgcgt ccgcgtggtg cgcgcccaga cgggattctc ttcctggccc
      421 agtcgtgcga gacagcgcag gaagcgccgg ccagcgggct gtcgagcagt ggccagccgg
      481 gccagcggca ggcacaggat ctcgaccacg tcgcgttcgc tgggcgtccg gcccgccgcc
      541 aggatcgcgt cgtacatctg gcggcgctcg gcattcacgc cgggcaacca gcgttgcagg
      601 atcgcttcga tcagcgcgtc gcggccgccg aagtggtagt gcagcacgcc ctggctgatg
      661 ccggcggtgc ggttgatgtc gcgcaacgac accgctgcca cgccgcgctc gccgaacagg
      721 cgttcggcgg tgtcgaggat gcgcttgcgg gtctgcgcgg acgggtccga acgcgggcgt
      781 ggcggcacga gggctcctgg cgtcgtttac agcaattcga tcgtaacctg cccgatgctg
      841 cccgtgaacg ggtacggcgc gcggtagtcc gcaggtgagg agccggtgtc cagcccgatg
      901 tcgaaggtct cgtcgatgga gaagaacgtt gccgtgcgcg tcacctcgcc gcgcgcgact
      961 tcggtgccgt cgaccagcag gcgtcccttg cccccgggca ccggacccgc cccggtggtc
     1021 gtgaattcgt agcgcagcac gtgttcgccg ggcgcgagcg ctgtcgtgcc gcgcagcgtg
     1081 atttcctcgg cgtcgaagat gcgcaggcga tagaccggta cgcccccctg gtcgaggtac
     1141 aacgaccagc ccgcgctgcg tccacccagc gtcgcgagca cgccgcttgc gccaccgtcg
     1201 ggaacgcgca gtcgtgcctc gatcgcgtgc gagcgcgtct tcgtgttcgg cgcctccttc
     1261 tcggccacag cgatcgtgcc ggcgtggaac gtgaagcggg tgcgatcgcc ggccaggctc
     1321 ggaaagcgca tgttctccgg cgtcgggttg tgcagcagca cgtcgttggc atcggcctgc
     1381 tgcgcgaaca gctgttgcag ttcgcgcagc ctggccggct cgcgcgccgc gaggtccgtg
     1441 ccctggctga agtccgcgcg caggtcgtag agctcccagc ggtcggcgtc gaagtcctcg
     1501 tcgcgcgcgg cgctgttcac ctgccacggc gcgcgtccgc gaaaggcgga ggccatccag
     1561 ccctcgtggt agatcgagcg gttgccgaag atctcgaagt actgcgtggc atggcgctcg
     1621 gcggctttcg cgtcgtcgaa cgagtaggcc aggctcacgc cgtccatcgg gcgctgcgcc
     1681 acgccatcga cgctcgcggg tgccgcgatg ccggtgactt cgaggatcgt cggcacgatg
     1741 tcgttcacat ggccgaactg cgaacgcaga ccgccgcggt cgcggatgcg ctcgggccac
     1801 gccagcacca tcgggttgcg cgtaccaccc aggtgcgagg ccacctgctt ggtccactgg
     1861 aacggcgtgt tggtgcccca ggcccagccc gagttgtaat gcgcgtaggt gttctcgccg
     1921 ctcagcgtct gcggggcatc gccaaagcgc gccacctccg agaccaggcc ctgcagatcg
     1981 cccatgtagt tgctgctgcc cttcaggccg ccctcggcgc ttgcgccgtt gtcgccgacg
     2041 atgtagacga acagcgtgtt gtcgtactgg tccatgtcct tcagcgcatc gcgcagtcgc
     2101 ccgacctgcg cgtcggtgtg tgcgaggaaa gcggcataga gctccatcag tcgcgccgag
     2161 acgcgttgtt cctccggtgt cagcgaatcc caggccggca gcgcgtcggg gcgtggcgtc
     2221 agcaccgcgt cgcgcggtat cacacccgcg gcctgctggc gcgcgaaggt ctcctcgcgc
     2281 agccggtccc agccctggtc gaacttgccg cggtagcgtt ccagccaggc tgccggcggt
     2341 tgcagcggcg cgtgcgcggc gccgggcgcg aagtagacga agaacgggcg cgcggggtcc
     2401 acggcacgct gcatctgcat ccacgcgacc gcctgctccg ccatgtcctc ggtgaggtgg
     2461 tagcccggcg cgcgctcgcg caccacgggc gtggtgccgt cgtagagcgt gggctcgtac
     2521 tggtccgttt cgccgccgag gaagccgtag aacttgtcga agccggcacc ggtcggccag
     2581 cgcgtgaacg gtcccgtttg cgaagcctcc cagtccggca ccaggtgcca cttgccccac
     2641 gccgaggtcg cgtagccgtt ctggccgagg atgcgcgcga tggtcgcggt gtcctcgcgc
     2701 agcacgccgc ggtagccggg gtacgaggtg gccgtgttca tcaccgaacc cacgccggcg
     2761 gcgtgcgagt tgcgcccggt gagcagggcc gcgcgcgtcg gcgagcagat tgccgtggtg
     2821 tggaagcggt tgtaacgcag cccgtcgtgt gccagcgcgt ccagcgccgg cgtctcgacc
     2881 gggccgccga aagtgccggc ggcgccgaag cccacgtcgt cgagcagcac caccacgatg
     2941 ttcggcgcgc cagccggcgc cagctggcgc gtcaccaccg gcgcctcggc cggtggggcg
     3001 gccgcggcag cgtactgcac catgccgagt cccgcgctca gcgcgaatag cgtcgccccc
     3061 aggcgcaggg agcctctggt gttgcgcatt tcctgtttct ccgttgtgcg tggtggatgt
     3121 cctgcggccg acgatacagg ctgcggaggg cgcctgcaac aaagcatttg gtttgagcac
     3181 ttgatgaaaa aaatgaaagt ggctacgatg cgcctcgccg ggtatcgcgc ctgccccgcc
     3241 gggggcgccc ggcgataacg attacaaccg gtccggcccc gccacggcgg cgccgggcaa
     3301 tccaacagga agcatccgca tgagcactcg catgtacccg gactgtgccg cgccccgcaa
     3361 accgctcgcc gccgccgttg gctggaccat ggcgctggcc tgctacgcgg ccatggatgc
     3421 cgcgccggcg cgcgcccagg gggctgcgct cgaggaagtc atcgttaccg cgcagaagcg
     3481 ccaggagaac gtgcaggaca ttcccgcgac ggtgaacgcg gtcacggcga gccagatcgc
     3541 ggattttcgc gtcgtgtcgc tcgacgacgt gcaggcgctg acgccgggcg tgctgatggt
     3601 gcgcaacgat gcgcgccggc agagcgtcac gatccgcggc atcacggccg acccggacaa
     3661 caacgcggca gcgccgatct cgtactactg gaacgatgcg ccgctgcgct cgcagcaggc
     3721 cttcacgacc ttcttcgaca tcgagcgtct cgaggtgctg cgcggtccgc agggtacctt
     3781 gcaggggcgc accgatccgg ccggcgcgat cctgatccac accaagcgtc ccgacctgca
     3841 agccgtgagc ggcaccgtgc gccagagctt ttccgacaat tccggcatgc agaccgaggc
     3901 cggggtcagc gtgccgctgg tgaaggacgt gttcggcgtg cgcctggccg gactctggga
     3961 cgagagtgat ggctcggagt acgagaacat cgtcaacggc caggacgaat cccagcagac
     4021 cggctcctat cgcatcagcc tggactggat gccgctggag gcgctcgaga tcagcctggt
     4081 ccacgagcgc tccgacgtgg acgccgaggt tcccgagggc gcgcagggcg acgtcggcaa
     4141 cgtgatcaat gccatcaccg cccagcgcaa tggcgcttcg ttcgtcttca accagacact
     4201 ggccgggatc aacaacccga atgcgctggc gggcgccgcg ttcgcggcgg cgccgagtgc
     4261 gttcatcccc ggccagacca acgcgggggc attggtccag accaacatcg cgcgtcgcgc
     4321 ggtggcgccg acctggctcg ccggcgtgtc gctggacgac gacgatcgcc agtccatcca
     4381 gctcgggcgc aacgatctgg acatgcgcag cgagttcacg gtgctgcgcg ccgtatggga
     4441 gctggagaat cacacgctca cctcgatcgg cagcctgaag gactggcgcc agaacaactg
     4501 gctggacatg aacttcgcct acctgagtcc tgtcgcgcag atgcagaaca cgcactccga
     4561 ggccgaggac tggtcgcagg agatccgctt cgccagcaac gacaatgcgt tctggaacta
     4621 cacggccggc atgttctggg aggattttga cggcttcacg aacaactaca cggatctcgg
     4681 tggtcccttc gcgatgtacg gcagcatggc cggcatcacc aacggcgaca tggtccagca
     4741 cctgcagatt cccaacggtc gcgagacgct ggcattcttc ctgcacaatc gcctcgagct
     4801 gagcgaggcg acgaacctgc agctcggtat ccgctggcag gaaatcgacg gcgagcagat
     4861 cgtgcgcgcg gaagtgatcg acaactacgc gccgcccgcg gaatccggtc ccgtgaccaa
     4921 cccgctgctg gcgggcgcac tcaatccgcg cctgaccagc gccggcgtgt ggaacatctt
     4981 caagggcaat atccgcaccg gcgacctgat cccgaacgag ctgcagacca ccgaggagcg
     5041 cgagtggacg gggggcgtga agctctcgca ctacctcaat gacgacgaca tggtctatgc
     5101 ctcgctcgac cgcagcttcc gtccgagcgg cgcgaccatc acgccgacgg cgctgtcggc
     5161 ggactccctg ctcttcgaca gcgagaccag cgcttcgctc gagctcggct acaaggccat
     5221 ggccttcgac ggcaatctgc gcttcagcgc cgcggcctat taccagcagt actccgatta
     5281 cctgccgcgg gcgagcaacg tgaacgcgct ggtcgcgacc ggcgcgggcc gcgtgccaca
     5341 ggtggtgcag ggggggctgg tgttcaatgc cgatgcgacc gtcatgggcg tggaggccga
     5401 atggacggcg ctgctcggag agcgcttcca gttcggcggt ggattgagct actccgaggc
     5461 caccttcgac gacgacgaac cggggccctg caaccggccg ttcacgccgg ccgagatcgc
     5521 cgatccgacg gtggagatcg cgacctgcga tatcggtggc gaggacgtga gctcgcagcc
     5581 caactggtcg gcgacgctca atgccgagta cacgctgccg ctgtccttcg gcgaactgtt
     5641 cgtgcggccg ctgcttgcct acaccggaga gcgcgaggac cggctggtcc ccgcccagag
     5701 ttttgacgat tatgtgctgg tgaacctcta tgccggcgtg cgcagcgagg acggcgtctg
     5761 ggagggaacc gtgtgggcca agaacctctt cgacgaggac gccaacagcg tcttcagcaa
     5821 tccgtacttc gggcccctcg gcggcaacag caatttcacg cgcgcggtca tggtgccgcc
     5881 gcgcctgctc ggcgtgaccg tgagttacaa cttcgccgga gggtgatggg atccatgccg
     5941 cgccgcctgc tcgccgtgcc gaccctgctc gccgcctgct ccggagcagt gtgcgcgggg
     6001 gagtcctatt ccccttacgc cggtgacgcg catcccgcgc gcgtttattg gggtgatacg
     6061 cacctgcaca cgaacatgtc ggccgacgcc aacatcgcgg gcgacatgcg catggggccc
     6121 gacgaggcgt accgcttcgc gaagggtgag acggtgcgag cgcagaacgg gatgagcgtg
     6181 cggctccagc gtccgctcga tttcctcgtc gtcgcggatc acgccgaata cctcggtctc
     6241 acggtgggcc tgctggccga cgacccgcaa ctgctggccg acccggtcgg caagcgctgg
     6301 cacgcgttgt tgaaagccaa taccgccaca cggatagaga gcgcggccga cgcgacgggc
     6361 tacacgaagg agatgatggt ggagtccttc ctgccgcagc gcgaagtgct gcgcgacgag
     6421 gccttcgccg cctcggcgtg gaagcaggtt gcggccaatg ccgaccgtca ctacagcccc
     6481 ggcaccttca cggccttcgc cggctacgag tggtcctcga tgccggaaat cgacaacctg
     6541 caccgcgtgg tcattttccg ggacggcttc gacaagacct cgcaggtgcg cgccttctcg
     6601 gccttcgaca gccaggatcc cgagcaactc tggcgctacc tcgaggagta cgaacgcatg
     6661 accggcggtg cggcgatcgc gataccgcac aactccaatg cgagcaatgg ccgcatgttc
     6721 gagttgaagg acatgcaagg acggccgctt acgcgcgatt acgccgagcg ccgggcgcgc
     6781 tgggaaccgc tgctggaggt cacgcagatc aagggcgaca gcgaggcgca tccgctgctc
     6841 tcgcccgacg atgccttcgc cgactacgag acctgggacc gcggcaacct gctgttctcg
     6901 aagcccaagg aaccgtggat gctgcagcac gagtacgcgc gctccgcgct gaagctcggc
     6961 ctgcagcagt atgcggcgct cggcgtgaac ccgttcaagt tcggcatgat cggctcgacc
     7021 gatgcgcata cggggctggc ggcggtggag gagaacaatt tctggggcaa ggtgattgcc
     7081 agcgaaccct cggccgagcg ctggcgcaaa ccctttttca agggcgaggc gctggaagtg
     7141 cgcaactccg aactcgccgc ctcgggctat gccgcggtct gggccacgga gaacacccgc
     7201 gaggccatct tcgccgcgat gaagcgacgc gagacctatg ccaccaccgg cccgcgcatg
     7261 acggtgcgct tcttcggtgg ctgggagttc gtgccgggcg atgagcacgc accggatccg
     7321 gcgcgccgcg gctatgccgg cggcgtgccg atgggcggcg acctcccggc accggcgacc
     7381 gcgggcgcgg ctcctggctt cctggtggcg gcgctgaagg atcccgaggg cgccaacctc
     7441 gaccgcattc agatcgtgaa gggctggcac gacgcggacg gcgaactgca cgagaaggtg
     7501 tacgacgtcg cgctcgccga cgggcgcggc ccgggcgcgg acgcggcctc gataggcagc
     7561 agcgtcgacg tggcgcgcgc gagttattcg aacagcatcg gcgacgcgga actggccgcc
     7621 gtgtggcggg atcccggctt cgacccggcg cagtcggcgt tctactacgc gcgcgtgatc
     7681 gagatcccga cgccgcgctg gaccgcgcgc gacgcggagt acttcggggt cgggatggat
     7741 cgtgaggtca cgatgatcac gcaggagcgg gcctatacct cgccgatctg gtacacaccg
     7801 gcgaacgagc gtcgctgatc ggacatcgcg gaggcaggag atgatcggaa gggttgcgaa
     7861 caaggtggcc atggtcacgg gcggtgcgag cggtctgggc gaggcgacat cgctactgct
     7921 ggcgcgcgag ggcgcgcgcg tcgcggtggc ggacgttgcg caggaggccg gcgagcacct
     7981 ggccgagcgc atccgcgccg aggtcggcga cgcgatgttc gtgccgctgg acgtcagcag
     8041 cgagagcgcg tgggacgcgg cgatggcgca ggtgcagcaa cgctgcggga agctgaacat
     8101 cctggtcaac aatgccggca tcgcgcccga gggcgacatg gaaatgtcct tcgaattgtg
     8161 gcgcaaggtc atgagcgtca atctcgacgg caccttcctc ggcacgcgcg cgggcatccg
     8221 cgcgatgcga tcgagcggcg aacccggttc gatcatcaat atctcctcga ccatggccat
     8281 gacggccttt cccaccacgg cggcctatag cgcgagcaag ggcggggtgc gcagcctgac
     8341 caaggccgcg gcgatgtact gcggcgcgca caggctgccg atccgcgtga actcgatcca
     8401 cccgggcatg tgcgtcacgc cgctggtcgc cggttactac gagaacaacc ccgaggccct
     8461 gcaggcgcag atcgacatgc atccgatcgg ccatctgggc accgcggaag atatcgccga
     8521 gggcgtgctg tacctggcat cggaggcctc gcgcttcgtg ctcggttccg agctcgtgat
     8581 cgacggcggc ttcctggcgc gctgagggcg ggcgcctgtc ccggcgtgca cacagggaca
     8641 acaggaacag aacatgacga tgaataacag ttccgtgttc cgcctgctgg cgacggcgtg
     8701 catcctgggc gcgctggcgg cgccgctgcg cgccggcacg gcgccctcgc cgatagatcc
     8761 gggccttgcg cccgagcgcg tcgccgcgtt gaccgagcag gccttctact ggggcatcaa
     8821 tatcgccggg ttctacgagc tgcgccatct ctacacgcag ctggaggggc atccggcctt
     8881 tcgcggcatc aaccggatgc agccgcacaa gcagctgttc gacgccagcg tgcgcatcgc
     8941 gaccacggtc aacgcctcga cgctctactc gggaggagcc ttcgacgtct cgcgcgaacc
     9001 gatcgtcgtc gaggccgggg cggtcggcga cggacgttac tggagcgtgc aggcggccga
     9061 ccagaacgcg aactggttct tcatggccgg ttcgcagttc agcggcaacg cggcccagcg
     9121 ctacctgatc ctcgggccga agtggcgcgg caggctgccc gccgcgttcc gctcgaccca
     9181 gatcgtccgc gcaagctcgg actccttcac gctcgccgtg cgcgtcgcgg tgaccacgcg
     9241 tgacgcgcag gacatgcgcg ccgcgggcgc cgtcatcgac aacgtgctgg tcgctccgct
     9301 cagccagtgg gaggccaacg gcggcaccct gccgccgctc gaaaggcagc cggtcgtgaa
     9361 gggcgactat cgtttctttc cccgcatgag cgcgattggc gatattggca agtcgatgac
     9421 gcccgtcgat tatctgcagc tgctcagcct cgcgatcaac gatcccgcgc tcacgaagcg
     9481 tacggactcg gccaaggaac tcgcgacgct cgcggcgctc gcgccgctcg gcctgcgcga
     9541 gggccttatc ttcgacccgg ccggcctcac cgccgagcag cagtccgcca tcgccagggg
     9601 ctttgcccgg gggcgtgcga acgccaagct cgcgctggaa aaatcactga tcgacatgaa
     9661 cggctggaag ctgcagtcga gcctgttcca cgacgatctc gactacgtgg cgaaagcagg
     9721 cgccaacgat gttgcgtggg gtacgcccgt gccgtatcag tcgcacagca tcgcctacgt
     9781 ctttcgcgac agcaaagggc gggcgctgga cggcagacac cgctacactc tgacgttcga
     9841 tctggagaat atgccgcccg tcactgagtt ctgggaactt ccggtctatg acgaatacgg
     9901 ctatttcatc gccaatgcga tcgatcgcaa cagcgtcacc agctatctgc acaaggccgg
     9961 tgcctacgca gagggtaacg gcaggctcac gttctacctg cagcccgaaa agccctcgga
    10021 tccggagcag gcgagaaact ggctgccgac gtcggccacg ggcggcttcc agctcgccgc
    10081 gcgcttctac gggccgatgg cgccgctgat cgacggctcc tacgcgatgc ccgccatcgt
    10141 gcgcagcgac gatgccgatt gacgcccgga tgccacgaga cagccccgga cacacgaaat
    10201 acaggagtgc aagttgatga ctgttgcccg ctcgacacgc atcgcaagtc gtcggtctcg
    10261 cctcggcggg gcgctgctcg cgctcgcgct cgcgctcgcg gcggcgcccg cgctggcgca
    10321 aaccgtgctg ccgcggccgg ccgagccctt caccggcaag atcggcacga cctatgccga
    10381 ctccacgccg gcgttcccgg cgccggtcac ggcgccggcc ggagcgccca acgtcgtgct
    10441 ggtcctgacc gacgatgtgg gcttcggcgc ggcgagcact ttcggcgggg cgattccgac
    10501 cccgcacctg gatcgactgg ccgaggaggg cttgcgcttc aaccgcttcc acaccaccgc
    10561 gatgtgctcg cccacgcgtg ccgcgctgct gaccgggcgc aatcatcacg cggtcggcaa
    10621 cggcatcgtg gcaaacctga cgacgggctt ccccgggtac aacaacctga tgccgaagag
    10681 cgcggcgacc atcgccgagg tgctgcgcca gcacggctac aacaccgcga tgttcggcaa
    10741 gcaccacaac gcgccggagg cgcacgtgtc cgcggccggc ccgttcgaca tgtggcccac
    10801 cggtctgggc ttcgagtact tcttcggctt catggctgcc gagaccaacc agttcacgcc
    10861 ggcgctctat cgtggcatca cgccggtgcc gaccctcgag gaaggcgtgc tggacaaggc
    10921 gctggccgac gatgcgatcc actggctgca ccagcagcag gccgcggcgc ccggcaagcc
    10981 cttcttcatg tattacgcga ccggcaccgc ccacgcgccg ctgcaggcgc cggcggactg
    11041 gatcgcccga ttcgagggca aattcgcggg tggctatgac cggatgcgcg aggaagtcgt
    11101 cgaacgccag aaacggatgg gcctggttcc cgcggatacc gtgaacacgc cgcggccgga
    11161 ggaaattgcc gcgtgggacg cgctcacgcc ggagcagaaa aagatcaacg aacgcttcat
    11221 ggaagtctat gccgcgatgc tcgcgtacca ggacgagcag gtcgggcgcc tgttcgccga
    11281 gatcgggcgc atggggttgc gcgacaacac gctggtgatc ttcatcgagg gcgacaacgg
    11341 ggcggcggcc gagggtggca tgcacggcag catgaatccg atgtccaatt tcgccaacgg
    11401 cacgcaggag agcgaggcga cgctgctggc gaatctcgag cggatcggcg gtccggaggc
    11461 ggttgctcaa tacggctacg gctggacatg ggccaccaat gcgccgtttc gcctggccaa
    11521 gcagtacgcc tcgcacctgg gtggcacgcg caacggcatg gtggtgtcgt ggccgcaacg
    11581 catcaagtcg agcggtatcc gccctcagtt cagccatgtc acggatatcg cgccgaccat
    11641 ctacgaagcc gccggcgtca cggcgccgga tacggtcaac ggcgtggagc agcagccgat
    11701 agacggcagc agcctgctgt acgcattcga caacgccgct gccgcggagc gccacgacac
    11761 gcagtacttc gagatgatgg gcaaccaggc gatctatcac cagggctggc tggcgagcag
    11821 cacgccggcg atcgccccgt gggctcgtga ccggcgcaac gacacgctgc cgagcgagta
    11881 tccgtgggag ctgtacgatc tgcgcgcgga tttctcgcag gcgcatgatc tggcgtcggc
    11941 gcacccggaa aaactcgcgc agatgaaggc cctgttcctg gaggaggccc ggcgcaacca
    12001 ggtgctgccg ctcgacaatc gcctgtcgat ggagcgcttc ctcgcggctt ccagccagca
    12061 gcgcaaggcg aacacctaca cctactgggg cgccggcgtc agcgtgccgg cggtcagtgc
    12121 cgcgccgctg ctgaaccgtt cgttcactat cagtgccgag atcgatgcgc ccgcgccggc
    12181 gcagggtgtc gtgctggcgc tgggcagccg gttcggcggc tggagtttct tcgtgcagga
    12241 cggcacgccg gtggcattga tggcggcctc ccagctcgag ggcgaccagt cgcgcgtggt
    12301 ggcgagcggc aggctgccgg ccggaaaatc ggtgctgagc taccagttcg tctccgatgg
    12361 cggcgtcaac gcgggcgggg aaatgcgcat cagcgtcgac gggcgcgaga tcggccgcgg
    12421 acgcatcgcg cgcacgatct cgaagctccc ggagatgacc gacacgctcg acatcggctt
    12481 cgacgccgac acgcccgtca ccgccgagta ccgcgacggg ggccgcttca acggcatgct
    12541 gagccgtgtg gatgtgagca tcgcaaattg aagcccggac ccggacggat cacgggtgga
    12601 gcgggtgcga cgcgaggaaa gccgacatga atacatcgaa gcggcgcgcc ggtggtattt
    12661 cattcggcgc ctggatctgc ttctgcgtgc tgctggcccc ggtgccgggt gcggcagcgg
    12721 gtgacgacgg cccgcgtccg ggcgcggcgg ccgaggaatc gggcgcgctg gcgtcttcgc
    12781 ggcgccgcgt cctgttcggc gaactgcacc tgcatacctc gtactccttt gatgccttcg
    12841 gcttcagccc gtcgcgcatc gatccggaca ccgcctaccg gttcgcccgc ggcgaagcag
    12901 tcgattacct gggcaagccg gcccgccgcc atgcgccgct ggatttcatg gccgtgacgg
    12961 atcatgcgga gtacatgggc ttcctgaaca cgatcgagga c
//
LOCUS       DAORUQ010000092        12951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4784_length_12951_cov_7.791873, whole genome shotgun sequence.
ACCESSION   DAORUQ010000092 DAORUQ010000000
VERSION     DAORUQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12951
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_12951_cov_7.791873"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLW13_09360"
     CDS             <1..286
                     /locus_tag="PLW13_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQY70624.1"
                     /translation="IEAEIPGVKREDVRVGVEDGVLTIRGHRELPRAAGAESHRHRTE
                     RTGGRFARSFALPEFADPERITARVEDGVLSITVAKRAAGGSRSIEIRVG"
     gene            complement(283..990)
                     /locus_tag="PLW13_09365"
     CDS             complement(283..990)
                     /locus_tag="PLW13_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HQY70625.1"
                     /translation="MNRTAAICRAAATVCALLLVAAPLATRLGLVSWKLGLPLTALAI
                     LGGAVLLLLALVLLALPAWRAARAPLAASALLAALPVLAGTALVAPGLGLPAIHDVST
                     DIADPPQFVALVAVRGPDANALARSPEVDAAQRNAWPGLGPLDSPLPPAEAFARAAAT
                     AHALGWRIHAQDAAAGQIEASATTFWFGFVDDVVIRVRPHAEGSRIDLRSVSRVGQGD
                     LGANARRIARFSAAFSG"
     gene            complement(987..1661)
                     /locus_tag="PLW13_09370"
     CDS             complement(987..1661)
                     /locus_tag="PLW13_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70626.1"
                     /translation="MKTRDRIIHASLELFNREGEPNVTTVDIANELDISPGNLYYHFK
                     GKEELVRELFERFYEQFNVILRAPVQNPLRLEDNWFYLVVVFEHMHAYRFLYRNISLI
                     LQRYEQIQRPFRRLIHLKQDAARAICVQLRTAGIMQIDDAGIDMLARSIALTITYWFN
                     FDNLLGDRPGDDEDLIHDGVLQALSLIAPHLGAMQREFMDTALAIHSRLGRAAVQATR
                     TKGGRK"
     gene            1773..2360
                     /locus_tag="PLW13_09375"
     CDS             1773..2360
                     /locus_tag="PLW13_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQY70627.1"
                     /translation="MARTRKRKPLESGETVARKIWLAGLGAYGKSVEDAHGQIDKASQ
                     EASKFFHDLVSKGQSIEDHSREALKERISEAKEMISEARERISEAADSNTRSVEEMIG
                     RVRERMGVDDAVHMRLDALARQVNALAQKVTGLSLRKGAAKGKARKPPARAAAKPAAR
                     KPAAKAPVRKSAAKPAARKTGATPARRGRPPKSAR"
     gene            complement(2817..3590)
                     /locus_tag="PLW13_09380"
     CDS             complement(2817..3590)
                     /locus_tag="PLW13_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70628.1"
                     /translation="MQDRLDMQGKVVIVTGGAKGVGAGISRSFLEAGASVAIVGRTPP
                     VELPACGANAASFWQADVRDYEQLSACTDAIVARYGRIDVLVNNAGGAPATDAATASP
                     RFSESIIRLNLLAPLNFSQLANRVMQQQESGGTIINIASVSAIRPSPGTAAYGAAKAG
                     LVNLCTSLAVEWAPRVRVVAVISGLVVTEQAHLHYGDEAGIAAVGRTIPLGRMAVPAD
                     IGNVCLFLASPLASYISGSAITVHGGGEAPAFLAASNAD"
     gene            3717..4415
                     /gene="npdG"
                     /locus_tag="PLW13_09385"
     CDS             3717..4415
                     /gene="npdG"
                     /locus_tag="PLW13_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HQY70629.1"
                     /translation="MSETTGAGSKRIAILGGTGALGTGLVRRWTQAGHAVIIGSRTLE
                     KAEAALAELKQVMAEWGATKLDVAAMENLDAARAADIVVLTVPFAHQKSTLEHVRPAL
                     EGKILVDVTVPLMPPKVGTVQLPPEGSAGMAAQQLLGDGVKVVSAFQNVAAHHLQEGH
                     GIECDVLVCGNDKDARAEVIGLVEACGMRGFHAGPIANSAAAEALTSVIININRAFKC
                     HAGIRITGLDSAGE"
     gene            4408..5169
                     /gene="cofE"
                     /locus_tag="PLW13_09390"
     CDS             4408..5169
                     /gene="cofE"
                     /locus_tag="PLW13_09390"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HQY70630.1"
                     /translation="MSNTLALIPLTGLPLVEPGDDIAAMLLAAAARCAGGLLDGDLLV
                     VAQKIVSKAENRYVDLDTVVPSARARELAGPADKDPRLIEVILSESREVMRQRPGALI
                     VEHRLGYVHANAGVDRSNVADPNKVLLLPLDPDASARQLRERARRDAGCCISVIINDS
                     AGRAWRNGIIGFAIGCAGFRPVVNRIGAPDLFGRKLEITEIAVADELAAAASFVMGQA
                     DEAIPAVLVRGAKLEPSEEGMAALLRARERDLFRQ"
     gene            5166..6116
                     /gene="cofD"
                     /locus_tag="PLW13_09395"
     CDS             5166..6116
                     /gene="cofD"
                     /locus_tag="PLW13_09395"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HQY70631.1"
                     /translation="MSAARRVLAISGGVGGAKLAFGLAALLDPAELLIVANTGDDFEH
                     LGLPICPDIDTLIYTLAGLADPLRGWGLAGETWQCMGALGTLGGPDWFRLGDRDLATH
                     LQRGALLRAGLSLSEATARLATALGVRHAIVPMSDAPVRTLVDTERGTFAFQEYFVRE
                     RCEPRVRGVRFDNAAAARPAPALGDWLEGPRRPLVVICPSNPWLSVDPVLAVPGLRER
                     LAALPVVAVSPIVGGRALKGPAAKMMAELGLPVSALEVARHYVGLADAFVIDREDEAQ
                     GDAIRALGMRVLVTDTVMSDAGSKRRLAADILGLLDTFGA"
     gene            6113..6826
                     /gene="cofC"
                     /locus_tag="PLW13_09400"
     CDS             6113..6826
                     /gene="cofC"
                     /locus_tag="PLW13_09400"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HQY70632.1"
                     /translation="MSPPALWVLVPLKNLGRAKERLSGALAALARRELVEAMARDVIE
                     ALQAVPVAPAHIVLVSDDDDVAALAAQYGVALFRPAAATADPLNAALTEAMRHACAEG
                     AGHVLVMHADLPLARAAQLRGLIDAHLAALEARGAPLATLVTDRAGEGSNCLLSTPPD
                     AVPYRFGAGSRARHHEAARAAGVQCVEHHAAGLERDIDQPEDLAELVAICQSAENAGG
                     AHSTRLLLRRGSEGNGHTF"
     gene            6845..9316
                     /gene="cofH"
                     /locus_tag="PLW13_09405"
     CDS             6845..9316
                     /gene="cofH"
                     /locus_tag="PLW13_09405"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HQY70633.1"
                     /translation="MLSRNPLDALAEAARCVDGLSDEQALALAGIHDTAALMHVAAGL
                     RDAAHGNVISYSRKVFIPLTHLCRDVCHYCTFAQTPKRLDSPYMPLEQVLASARDGAR
                     LGCKEALFTLGEKPELRYRAAREALQTLGFATTIEYLAHVARAVLDETGLFPHINAGN
                     MDAREIALLRPLAPSMGIMLESASERLCAKGMPHHGSPDKAPAARLHTMRLAGEARVP
                     FTSGILIGIGETRAERIESLLALRAIHREHGNLQEVIVQNFRAKPGTRMASAPEPDLE
                     ELLWSIAVARLILGPAMNIQAPPNLSPGVLARLVEAGINDWGGVSPVTPDFVNPEAPW
                     PHLGQLAAETAGAGKFLQERLTIYPSYVREAARWVDESLHARLLAATDAEGFPRIDDW
                     LTGSSTELPALERELLAGKGHGGGVSARIEALLARAGAGEELDEAGVTALLGARGADF
                     ARVCAQADALNRRLHGERVSYVVNRNINYTNVCYFKCQFCAFSKGKAHDHLRGTPYDL
                     DDAELARRVTEAWARGATEVCLQGGIHPAYTGQKYLDVCRLVKEAAPGMHIHAFSPLE
                     IWQGAKTLGLPLGDFIAQLREAGLATLPGTAAEILHDEVRAVLCPDKIDSAQWLEVME
                     AAHSQGVRTTATMMYGHIEQPRHQAHHLLAIRELQRKYHGFTEFVPLPFVAGEAPMYL
                     RGRARRGPTFREAVLLHAVARIVLYPDVPNIQASWVKMGAEGVAACLHAGANDVGGTL
                     MNESITRAAGAAHGEEFPPAELEQLIRDAGRQPWQRTTLYEAAPPERHETALRAAPLT
                     EYVNTPVRRAAPGARKGGVIARAAH"
     gene            9365..10264
                     /locus_tag="PLW13_09410"
     CDS             9365..10264
                     /locus_tag="PLW13_09410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70634.1"
                     /translation="MEYWLSITTIPEQDQLLALARIAEEAGFDGITVADHVLMPTRIA
                     SRYPYTEDGAIWWPADTPWPDPWVTLAAMGAVTTKLRLASNICLAALRDPATLAKSVS
                     TAAVLTGGRIVLGVASGWLREEYELLGVDFATRGRRLDELLTVARKFWSGRPVTHEGE
                     FFCYAESIMCPAPAAPVPVWCGGAVPAALGRAVRNDGWLGLPLDRAGAAPVLAALLQQ
                     RDAAGLARENFAINIALTEAHEPQAIADVAAMGVTGLMIMSPWLPNPFFAAPWYRADA
                     DPARLETKREAIMRFADQVLARR"
     gene            10305..10748
                     /locus_tag="PLW13_09415"
     CDS             10305..10748
                     /locus_tag="PLW13_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70635.1"
                     /translation="MAATPAQVREVIGKYMEAWATGNKELLLSIFAEDATWEDPVGSP
                     AFVGLEGVSKFWDFARQGADEGRTISPRIDQVIACGNEGILRFTMQVRVPAQNQGLDL
                     AVVDHFVLNDAGKIAKARAFWDESCAGVPAGMDFFAPNIEQAYTQ"
     gene            complement(10762..11142)
                     /locus_tag="PLW13_09420"
     CDS             complement(10762..11142)
                     /locus_tag="PLW13_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HQY70636.1"
                     /translation="MSGQNPYQLLGGEDGLRRLCAAFYRIMDESSEATGIRAMHGESL
                     AAIEEKLFEYLSGWMGGPPLYVRRTGTVCLTKPHQPYAIGAAERDQWLLCMDRALEEV
                     GASAEVKDMLREPMFRIADMMVNR"
     gene            11220..11912
                     /gene="ung"
                     /locus_tag="PLW13_09425"
     CDS             11220..11912
                     /gene="ung"
                     /locus_tag="PLW13_09425"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQY70637.1"
                     /translation="MNGATSRIDLEPSWLAEIGNEFEQPYMRQLREFLRAEKAAGKLI
                     YPASANWFNAFRCAPFASVEVVILGQDPYHGPGQAHGLCFSVPRGVDVPPSLRNIFQE
                     LKRDLDLPAPAHGCLESWATQGVLLLNSVLTVERGRAASHQGRGWERFTDRVVEVLSA
                     RREHLVFLLWGSYAERKGRIIDRERHLVLSSPHPSPLSAHRGFIGNGHFSAANRYLEA
                     HGKRPIDWRLPP"
     gene            complement(12490..>12951)
                     /locus_tag="PLW13_09430"
     CDS             complement(12490..>12951)
                     /locus_tag="PLW13_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70638.1"
                     /translation="LGSLSGPPEVWFMRSSRLHGAARAGLRALMLIATLLATGTVQAL
                     SLGNASLQSRVGEPLRVSIPLGSLGALTQEEIIVGRAPEADYARFGIDPAALSTPLRF
                     ELLVDKRGNASVEVRSEKPLSEPFIDVVVEVRWPTGRLVREYTLLLDLPPR"
BASE COUNT         1954 a         4419 c         4672 g         1906 t
ORIGIN      
        1 gatcgaggcc gagatacccg gcgtgaagcg cgaggacgtg cgcgtcggcg tcgaggatgg
       61 cgtgctgacg atccgggggc atcgcgagtt gccgcgcgcc gctggcgccg agagccatcg
      121 gcaccgcacg gagcgcaccg gcgggcgttt cgcgcgcagc ttcgcgctgc cggagttcgc
      181 ggatcccgag cgcatcacgg cgcgggtcga ggatggcgtg ctctcgataa ccgtcgccaa
      241 gcgtgccgcg ggcggcagcc gcagcatcga gatccgcgtc ggctagccgg agaacgccgc
      301 gctgaagcgc gcgatgcgcc tggcgttggc gccgaggtcg ccctggccga cgcggctcac
      361 cgagcgcagg tcgatgcgcg agccctcggc gtgcggcctg acgcggatca cgacgtcatc
      421 cacgaatccg aaccagaagg tggtcgcgct ggcctcgatc tggcccgccg cggcgtcctg
      481 cgcgtgaatc cgccaaccca gggcgtgtgc ggtggcggca gcgcgcgcga acgcctccgc
      541 cggcggcagc ggcgagtcca ggggccccag tccgggccag gcgttgcgct gggcggcatc
      601 cacctcgggt gagcgtgcca gcgcgtttgc atccgggccg cgcacggcta cgagcgccac
      661 gaactgcggc gggtccgcga tatcggtgct gacgtcgtgg atcgccggca gcccgaggcc
      721 gggcgcaacc agggcagtgc cggcgagcac cggcagcgcg gccagcaacg cgctcgccgc
      781 cagcggtgcg cgcgcggcgc gccacgccgg gagcgccagc agcaccagcg cgagcagcag
      841 cagcacggcg ccacccagga tggccagcgc ggtcagcggc agcccgagct tccaggacac
      901 caggcccaag cgcgtggcca gcggcgcggc gacgagcagc aatgcgcaca cggtggccgc
      961 ggcgcggcaa atggcggcgg tgcgattcat tttcttcctc cctttgtgcg cgtggcctgc
     1021 acggcggcac gacccaggcg cgagtggatc gccagcgcgg tgtccatgaa ttcgcgctgc
     1081 atcgcgccga ggtgcggcgc gatcagcgac agcgcctgca gcaccccgtc gtggatcagg
     1141 tcctcgtcat cgccggggcg atcgccgagc aggttgtcga agttgaacca gtacgtgatc
     1201 gtgagcgcga tgctgcgcgc cagcatgtcg atgcccgcgt cgtcgatctg catgatgccg
     1261 gccgtgcgca actgcacgca gatggcacgc gcggcgtcct gcttgaggtg gatcaggcgc
     1321 ctgaacgggc gctggatctg ctcgtagcgc tgcagtatca gcgagatgtt gcggtacagg
     1381 aagcggtagg cgtgcatgtg ctcgaacacc accacgaggt agaaccagtt gtcctccagg
     1441 cgcaggggat tctgcacggg tgcgcgcagg atcacgttga actgctcgta gaaacgctcg
     1501 aacagctccc ggaccagttc ttccttgccc ttgaagtggt agtacaggtt gcccgggctg
     1561 atgtcgagtt cgttggcgat atcgaccgtg gtgacgttgg gctcaccctc gcggttgaac
     1621 agctccaggc tggcgtgaat gatgcggtcg cgtgttttca tttgccggaa cattactggt
     1681 atgttgttgc caattccagc cgttagcatg cgctggtcga aaaatgcagc ggttgccccg
     1741 gcgccgcgcg gcacccaacg gaggacacgg cgatggcacg cacacgcaag cgcaaaccgc
     1801 tcgagagcgg agagaccgtg gcccgcaaga tctggctcgc gggcctcggc gcctacggca
     1861 agagcgtcga ggacgcacac ggacagatcg acaaggcgag ccaggaggcc tcgaagttct
     1921 tccacgacct ggtgagcaag ggacagtcga tcgaggatca cagccgcgag gcactcaagg
     1981 agcgcatcag cgaggcgaag gaaatgatca gcgaggcgcg cgagcgcatc agcgaggcgg
     2041 ccgacagcaa cacccgcagc gtggaggaga tgatcgggcg cgtgcgcgag cgcatgggcg
     2101 tcgatgatgc cgtgcatatg cgtctcgatg cgctggcgcg gcaggtcaat gcgctggcac
     2161 agaaagtcac gggactgtcg ttgcgcaagg gcgcggcgaa gggcaaggcg cgcaagccgc
     2221 cggcccgggc ggccgcgaaa ccggctgcgc gcaagcccgc ggcgaaggcc ccggtccgca
     2281 agtccgcggc gaagcccgcc gcacgcaaga cgggcgcgac gccggcccgt cgtggccggc
     2341 cgccgaaatc cgcgcgctga ggcggtcccg cgcgctcagg ctcccatcaa actcgctcgt
     2401 ctgcgggaac tgcctgcccc gggtaggtcc ggattcatcc ggacattgtc cggttgaaga
     2461 gggtgtcgca aaaccatctg tgctcgggaa gcggcccgac aatccggcgt atcgcgagcc
     2521 cctcgccgtg gtggaagccc tcgaaaaggg gtgaattacc ggatttgcca gatgcggcac
     2581 acggtctacc gagcggctgt gctgcccgct gttggcttgc ggggccttgc gccgcaggac
     2641 gcggcgcgga gctacaggga cgtactcgtg cgtgccccgc aggacaatgg cgggcagcac
     2701 ggccagcgac gcacgaagcc gctggttcgc aacgtaaagg ctgttccgac atcctctaaa
     2761 acccgaacta cggtttgctg cgcggtagtg caccacacgc cggggtgcgc ggactctcag
     2821 tcggcgttcg atgccgccag gaaggccggc gcctcgccgc cgccgtgcac cgtgatcgcg
     2881 ctgccggaga tgtaactcgc cagcggcgag gccaggaaca ggcacacgtt gccgatgtcg
     2941 gccggcaccg ccatgcgccc gagcgggatc gtgcgcccca cggcggcgat gccggcctca
     3001 tcgccgtagt gcaggtgcgc ctgctcggtc acgaccagcc ccgagatcac cgcgaccaca
     3061 cgcaccctgg gcgcccattc caccgcgagc gaggtgcaca ggttcaccag gcccgccttc
     3121 gccgcgccgt aggcggcggt tcccggcgag ggacggatcg cgctgacgct ggcgatgttg
     3181 atgatggtgc cgccgctctc ctgctgctgc atgacgcggt tggccagctg cgaaaaattg
     3241 agcggggcca gcaggttcag ccggatgatc gactcggaga agcgcggcga ggcggtcgcg
     3301 gcatcggtgg ccggcgcgcc gccggcgttg ttgaccagca cgtcgatgcg cccgtaccgc
     3361 gccacgatgg cgtccgtgca ggcgctcagc tgctcgtagt cgcgcacgtc ggcctgccag
     3421 aagctcgccg cattggcccc gcaggcggga agctccacgg gcggggtgcg cccgacgatg
     3481 gccacgctcg cgcccgcctc gaggaagctg cggctgatgc cggcgccgac gcccttggcg
     3541 cctccggtca cgatcacgac tttgccctgc atgtccagcc tgtcctgcat ttccgcctcc
     3601 cgggtcgaga attcacttgc gccgctggtt gcgactagaa tggcgcgcca ttatagccag
     3661 ccctgcgcat ggcgcggcgg gtcggcggtt cgatacacag acatcgtgga gggtgcatga
     3721 gcgagacgac gggtgcgggt tcgaagcgga ttgcgattct gggcggcacc ggcgcgctgg
     3781 gcaccgggct ggtgcggcgc tggacccagg ccgggcacgc ggtgatcatc ggttcgcgca
     3841 cgctggagaa ggccgaggcc gcgctcgccg agctgaaaca ggtcatggcc gaatgggggg
     3901 caacgaagct cgacgtggcc gcgatggaga acctcgacgc cgcccgcgcg gccgacatcg
     3961 tggtgctgac cgttcccttc gcgcaccaga agagcacgct cgagcacgtg cgcccggcgc
     4021 tggagggcaa gatcctcgtc gacgtcacgg tgccgctgat gccgcccaag gtgggtacgg
     4081 tgcagctgcc gcccgagggc tccgcgggca tggccgccca gcagctgctc ggcgatgggg
     4141 tcaaggtcgt gtcggcgttc cagaacgtcg ccgcccacca cctgcaggaa ggccacggca
     4201 tcgagtgcga cgtgctggtc tgcggcaatg acaaggacgc gcgcgccgag gtgatcggcc
     4261 tggtcgaggc ctgcggcatg cgcggcttcc atgccggccc gatcgccaat tcggccgcgg
     4321 ccgaggcgct gacctcggtg atcatcaaca tcaaccgcgc cttcaagtgc catgccggca
     4381 tccgcatcac cgggctcgat tcggccggtg agtaacacgc tcgcgctgat cccgctgacc
     4441 gggctgccgc tggtggagcc cggtgacgac atcgcggcga tgctgttggc ggccgccgcg
     4501 cgctgcgccg gtggcctgct cgacggcgac ctgctggtgg tggcgcagaa gatcgtctcc
     4561 aaggccgaga accgctacgt cgacctggat acggtggtgc cctcggcgcg tgcccgcgag
     4621 cttgccgggc cggccgacaa ggacccgcgc ctgatcgagg tgatcctctc ggagtcgcgc
     4681 gaggtgatgc gccagcgccc cggcgcgctg atcgtcgagc accgtctcgg ctatgtgcac
     4741 gccaacgcgg gagtcgaccg ctccaacgtg gcggacccga acaaggtgct gttgctgccg
     4801 ctcgaccccg acgccagcgc gcggcaattg cgcgagcgcg cgcggcgcga cgccgggtgc
     4861 tgcatcagcg tgatcatcaa cgacagcgcc ggacgcgcct ggcgcaacgg catcatcggc
     4921 ttcgcgatcg ggtgcgcggg tttccgtccg gtggtgaacc gcatcggcgc gccggacctg
     4981 ttcgggcgca agctcgagat caccgagatc gcggtggccg acgagctggc ggccgccgcg
     5041 tccttcgtga tggggcaggc cgacgaggcg atcccggccg tgctggtgcg cggtgcgaag
     5101 ctggagcctt ccgaggaggg catggcggcc ctgctgcgcg cgcgcgagcg cgacctgttc
     5161 cgccagtgag cgctgcgcgc cgcgtcctcg cgatctcggg cggcgtgggc ggcgccaagc
     5221 tggccttcgg tctcgcggcg ctgctcgatc ccgccgaact gctgatcgtc gccaacacgg
     5281 gcgatgattt cgagcacctc gggctgccga tctgcccgga catcgacacg ctgatctaca
     5341 cgctggcggg cctggccgat ccgttgcgcg gctgggggct cgcgggcgag acctggcagt
     5401 gcatgggggc gctgggcacg ctcggcggcc cggactggtt ccgcctcggg gatcgcgatc
     5461 tcgccacgca cctgcagcgc ggcgcgctgc tgcgcgcggg gctcagcctg agcgaggcga
     5521 cggcgcgcct tgccacggca ctcggcgtgc gtcacgcgat cgtgccgatg agcgatgcgc
     5581 cggtgcgcac gctggtcgac accgaacgcg gcaccttcgc cttccaggaa tatttcgtgc
     5641 gcgagcgctg cgagccgcgg gtgcgcggcg tgcgtttcga caatgcggcc gccgcccggc
     5701 cggcgccggc gctcggcgac tggctcgagg gcccgcggcg gccgctcgtg gtgatctgcc
     5761 cctcgaatcc ctggctcagc gtcgatccgg tgctggccgt gccggggctg cgcgaacgcc
     5821 tcgccgcgct gcccgtggtc gcggtgtcgc cgatcgtggg cggacgcgcg ctcaaggggc
     5881 cggcggccaa gatgatggcc gagctcgggc tgccggtctc ggcgctcgag gtggcgcgtc
     5941 actacgtcgg gcttgccgat gccttcgtga tcgaccgcga ggacgaggcc cagggcgatg
     6001 cgatccgcgc cctgggcatg cgcgtgctgg tgaccgatac cgtgatgagc gatgccggca
     6061 gcaagcgccg gctcgccgcg gatattctcg ggcttctgga taccttcggc gcatgagccc
     6121 gcccgcgctg tgggtgctgg tgccgctgaa gaacctggga cgcgcgaagg agcgcctgtc
     6181 cggcgcactg gcggcgctcg cgcggcgcga gctcgtcgag gcgatggccc gcgacgtcat
     6241 cgaggcgctg caggcggtgc cggtggcgcc ggcccacatc gtgctggtct ccgacgacga
     6301 cgacgtggcc gcgctcgcgg cgcagtacgg cgtggccctg ttccggcccg ccgccgcgac
     6361 ggcggatccg ctgaatgccg cgctcaccga ggccatgcgc cacgcctgcg ccgagggcgc
     6421 cgggcacgtg ctggtgatgc acgccgacct gccgctggcg cgcgcggcgc aactgcgcgg
     6481 cctgatcgac gcgcacctgg cggcgctgga ggcgcgtggc gcaccgctcg ccaccctggt
     6541 caccgatcgt gccggcgagg gcagcaactg cctgctgagc acgccaccgg atgccgtgcc
     6601 gtaccgcttc ggcgccggca gccgcgcgcg tcaccacgag gccgcgcgcg cggccggggt
     6661 gcagtgcgtg gagcatcacg ctgccggcct cgagcgggac atcgatcaac ccgaggacct
     6721 ggccgagctt gtcgccatct gccaaagcgc agagaatgcc ggcggagccc actccacgcg
     6781 gttgcttctg cgccgtggca gcgagggcaa tggccacacc ttttgaggta gtaccgacgt
     6841 gcagatgctg agcaggaatc ccctggatgc gctggccgag gcggcgcgtt gcgtggacgg
     6901 tctgtcggat gaacaggcgc tcgcgctggc cggaatccat gacaccgctg cgctgatgca
     6961 cgtcgccgcc ggactgcgcg atgccgcgca cggcaacgtg atcagctatt cgcgcaaggt
     7021 gttcatcccg ctcacgcacc tgtgccgcga cgtctgccac tactgcacct tcgcccagac
     7081 gcccaaacgc ctggattcgc cctacatgcc gctcgagcag gtgctcgcga gcgcgcgcga
     7141 cggcgcgcgg ctcggctgca aggaggcgct gttcacgctg ggagagaaac ccgagctgcg
     7201 ctatcgcgcc gcgcgcgagg cgctgcagac gctgggcttc gccaccacga tcgagtacct
     7261 cgcgcatgtg gcccgcgcgg tgctcgacga gaccggcctg ttcccgcaca tcaacgcggg
     7321 caacatggac gcgcgcgaga tcgcgttgct gcgcccgctc gcgccctcga tggggatcat
     7381 gctggagtcg gcctccgaac ggctgtgcgc caagggcatg ccgcaccacg gctcgcccga
     7441 caaggcgccc gcggcacgcc tgcataccat gcgcctcgcc ggcgaggcgc gcgtgccgtt
     7501 cacctccggc atcctgatcg gtatcggcga gacgcgcgcc gaacgcatcg agtcgctgct
     7561 ggccttgcgc gccatccacc gtgagcacgg caacctgcag gaagtgatcg tgcagaattt
     7621 ccgcgccaag ccgggcaccc gcatggccag cgcgccggag cccgatctcg aggagctgct
     7681 gtggagcatc gcggtggcgc ggctgatcct cggcccggca atgaacatcc aggcgccgcc
     7741 gaatctcagc cccggcgtgc tggcgcgcct cgtcgaggcg ggcatcaacg attggggcgg
     7801 cgtgtcgccc gtgacgcccg acttcgtcaa tcccgaggcg ccgtggccgc acctcgggca
     7861 gctggcggcc gagaccgcgg gcgcgggcaa gttcctgcag gagcgcctga cgatctatcc
     7921 gtcctacgtg cgcgaggccg cgcgctgggt cgatgagtcg ctgcacgcgc gcctgctggc
     7981 cgcgaccgat gccgagggct tcccgcgcat cgatgactgg ctgaccggtt cgtccacgga
     8041 gctgcccgcg ctggagcgcg aactgctggc gggcaagggg cacggcggtg gcgtgagcgc
     8101 gcgcatcgag gcgctgctgg cgcgcgccgg ggccggcgag gaactggatg aggccggtgt
     8161 cacggcgctg ctgggcgcgc gcggcgcgga tttcgcgcgc gtctgcgcgc aggccgacgc
     8221 gctgaaccgc cgcctgcacg gcgagcgcgt cagctacgtc gtgaaccgca acatcaacta
     8281 caccaacgtg tgctacttca agtgccagtt ctgcgccttc tccaagggca aggcgcacga
     8341 tcacctgcgc ggcacgccct acgatctcga cgacgccgaa ctcgcgcggc gcgtgacaga
     8401 ggcctgggcg cgcggcgcca ccgaggtctg cctgcagggt ggtatccatc cggcctacac
     8461 cggccagaaa tatctcgacg tgtgccgcct ggtgaaggag gcggcgcccg gtatgcacat
     8521 ccacgccttt tcgccgctgg agatctggca gggcgcgaag acgctgggcc tgccgctcgg
     8581 ggacttcatc gcgcagctgc gcgaggcggg cctggcgacg ctgccgggca ccgccgccga
     8641 gatcctgcac gacgaggtgc gcgcggtgct gtgccccgac aagatcgaca gcgcgcaatg
     8701 gctcgaggtc atggaggccg cgcacagcca gggcgtgcgc acgaccgcga ccatgatgta
     8761 cggccacatc gagcagccgc gccaccaggc gcatcacctg ctcgcgatcc gcgagctgca
     8821 gcgcaagtat cacggcttca cggagttcgt gccgctgccg ttcgtggccg gcgaggcgcc
     8881 gatgtacctg cgcggtcgcg cgcggcgcgg gcctaccttc cgcgaggccg tgctgctgca
     8941 cgcggtggcg cgcatcgtgc tgtacccgga cgtgccgaat atccaggcct cgtgggtgaa
     9001 gatgggtgcc gagggtgtgg cggcctgcct gcacgcgggc gccaacgacg tcggcggcac
     9061 gttgatgaac gaatcgatca cgcgtgccgc gggcgcggcg catggcgagg aattcccgcc
     9121 cgccgaactc gagcagctga tccgcgacgc cgggcgccag ccgtggcagc gcaccacgct
     9181 gtacgaggca gcgccgccgg agcgccacga gacggcgctg cgcgcggcgc cgctcacgga
     9241 atacgtgaac acgccggtgc ggcgcgccgc accgggggcg cgcaagggcg gcgtgatcgc
     9301 gcgcgcggcg cactgagggc gcgcgaggcg ccgcttttgc cacgaacagg gagcgcacgt
     9361 tcgcatggaa tactggctgt cgatcaccac gattcccgag caggaccagc tgctcgcgct
     9421 ggcacgcatc gccgaggagg ccggcttcga cggcatcacg gtggccgacc acgtgctgat
     9481 gccgacacgc atcgcgagcc gctacccgta caccgaggac ggcgcgatct ggtggcccgc
     9541 ggacacgccg tggcccgatc cctgggtcac gctggccgcg atgggtgccg tgaccacgaa
     9601 actgcggctc gcctcgaaca tctgcctggc ggcgctgcgc gacccggcga cgctggccaa
     9661 atcggtttcc accgcggcgg tgctcacggg cgggcgcatc gtgctcggcg tggcctcggg
     9721 ctggctgcgc gaggaatacg agctgctcgg cgtggatttc gccacgcgcg gacgccgcct
     9781 cgacgagctg ctgacggtcg cgcgcaagtt ctggtccggg cggccggtga cgcacgaggg
     9841 cgagtttttc tgctacgcgg aatccatcat gtgccccgcg ccggcggcgc cggtgccggt
     9901 gtggtgcggc ggcgcggtgc ccgcggcgct ggggcgcgcg gtgcgcaacg acggctggct
     9961 cggcctgccg ctggatcgcg ccggcgcggc gccggtgctc gcagcactgt tgcagcagcg
    10021 cgacgccgcg gggctggcgc gcgagaattt cgcgatcaac atcgcgctca ccgaggcgca
    10081 cgagccgcag gccatcgcgg acgtggcggc gatgggcgtc accgggctga tgatcatgag
    10141 cccgtggctg ccgaacccgt tcttcgcggc gccgtggtac cgcgccgatg ccgatcccgc
    10201 gcgtctcgag accaagcgcg aggcgatcat gcgtttcgcc gaccaggtgc tggcgcggcg
    10261 ctgaaacgat tcattccatc accacgacat caaaaggagt tctcatggca gccacacccg
    10321 cgcaggtacg cgaagtcatc ggcaagtaca tggaggcctg ggccaccggc aacaaggaac
    10381 tgctgctcag cattttcgcc gaggacgcga cctgggagga tcccgtgggc tcgccggcct
    10441 tcgtcgggct cgagggcgtg tccaagttct gggatttcgc gcgccagggt gccgatgagg
    10501 gacgcacgat ctcgccgcgc atcgaccagg tgatcgcctg cggcaacgag ggcatcctgc
    10561 gtttcacgat gcaggtgcgc gtccccgcgc agaaccaggg gctcgacctg gcggtcgtcg
    10621 atcacttcgt gctgaacgac gccggcaaga tcgcgaaagc gcgcgccttc tgggacgaga
    10681 gctgcgcggg tgtgcccgcg ggaatggact tcttcgcacc gaacatcgag caggcgtaca
    10741 cgcagtagcg tgtcgccccg ctcagcggtt caccatcatg tcggcgatgc ggaacatcgg
    10801 ctcgcgcagc atgtccttca cctcggcaga ggcgcccacc tcttccagcg cgcggtccat
    10861 gcacagcagc cactggtcgc gttccgcggc cccgatggca tagggctggt gcggcttcgt
    10921 caggcagacc gtgccggtgc ggcgcacgta gagcggcggg ccgcccatcc agcccgagag
    10981 gtactcgaag agtttctcct cgatggcggc gagcgactcg ccgtgcatcg cgcgtatgcc
    11041 ggtcgcctcg gacgactcgt ccatgatgcg atagaacgcg gcgcacaatc tgcgcaaccc
    11101 gtcttcgccg ccgagcaact ggtagggatt ctggccgctc acgtcgtctc ctcctcggat
    11161 gtggcgcgga tgatagccgc tcgcgggacg ccgtgcatcg tccgaagaag gtacggcgca
    11221 tgaacggcgc gacctcgcgc atcgacctgg aaccgtcgtg gctggccgag atcggcaacg
    11281 agttcgaaca gccctacatg cggcagctgc gcgaattcct gcgcgccgag aaggctgccg
    11341 gcaagctcat ctatccggcc agcgcgaact ggttcaacgc ctttcgctgt gcgccgttcg
    11401 cctcggtgga ggtcgtgatc ctcggccagg atccctacca cggaccgggg caggcgcacg
    11461 ggctgtgctt ctcggtgccg cgcggcgtcg atgtgccgcc gtcgctgcgc aacatcttcc
    11521 aggaactgaa gcgcgacctc gatctgccgg cgcccgcgca cggctgcctg gagagctggg
    11581 ccacgcaggg tgtgctgctg ctcaacagcg tgctgaccgt ggagcgcggc cgcgccgcct
    11641 cgcaccaggg caggggctgg gagcgattca cggaccgcgt ggtggaggtg ctgagcgcgc
    11701 ggcgcgagca cctggtgttc ctgctgtggg ggagttacgc cgagcgcaag ggacgcatca
    11761 tcgatcgtga gcgccacctc gtgctgtcct cgccgcaccc ctcgccgttg tcggcgcatc
    11821 gcggcttcat cggcaacggg cacttctcgg cggcgaaccg ctacctcgag gcgcacggca
    11881 agcggcccat cgactggcga ctgccgccgt agttcctcat tcatccggac aagccgtggg
    11941 tccggattca tccggacagt gcccgcctta ggccgcattc atgcggacaa ttgttcggct
    12001 gaagagggtg ttgcaaaacc atctgtgctc gggagagcgg tctggcgcgt cgctggctgt
    12061 gctgcccgcc attgtcctgc ggggcacgca cgagtacgtc cctgtagctc cgcgccgcgt
    12121 cctgcggcgc aaggccccgc aagccaacag cgggcagcac agccgctcgg tcgagcgcgt
    12181 gccgcatcgg gcaaatccgg gaatgcgcgc cttttcgggg gtttccacca cggcgagggg
    12241 ctcgagcacc catcggagcg gcggacactc tgtcgccagg acggcgacag gtagcctaca
    12301 tggatgtatt cacggcgtgt ccgcggctcc gatgggtgct cgagcgccga tggctcgcga
    12361 tacgccggat tgtcgggccg cttcccgagc acagatggtt gtgcgacacc ctttaaagcc
    12421 gcacctgcgg tcggacgttt tgcttcgcga gcgcgcacca cacatcgggg tgcgcgggct
    12481 atcaggcgct cagcgcggcg gcaggtcgag cagcagcgtg tattccctga cgaggcgccc
    12541 ggtcggccag cgcacctcga ccaccacgtc gatgaacggt tcgctgagcg gcttttcgct
    12601 gcggacctcg acactggcgt tgccgcgctt gtcgacgagc agttcgaagc gcagcggggt
    12661 gctgagcgcg gcggggtcga tgccgaagcg cgcgtaatcg gcctccgggg cacgcccgac
    12721 gatgatctcc tcctgcgtga gcgcaccgag gctgccgagc gggatcgaca cgcgcagcgg
    12781 ctcgccgacg cgcgactgca ggctggcgtt gcccaggctg agcgcctgca ccgtgccggt
    12841 ggcgagcagg gtcgcgatca gcatcagggc gcgcaggccc gcgcgcgcgg cgccgtgcaa
    12901 tctggatgaa cgcatgaacc acacctctgg cgggccggac agtgagccga g
//
LOCUS       DAORUQ010000093        12933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4798_length_12933_cov_5.924522, whole genome shotgun sequence.
ACCESSION   DAORUQ010000093 DAORUQ010000000
VERSION     DAORUQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4798_length_12933_cov_5.924522"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..612
                     /locus_tag="PLW13_09435"
     CDS             301..612
                     /locus_tag="PLW13_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70639.1"
                     /translation="MPTNDTDHRLFGEYLHWIAHKELALGAVRAELWASAVVQCAGDE
                     DKARGIYLESRAQSLREEASIAEKFLRLLRQSEDSAVQVHDAVVFYLARPAAGRVESP
                     R"
     gene            complement(625..1188)
                     /locus_tag="PLW13_09440"
     CDS             complement(625..1188)
                     /locus_tag="PLW13_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HQY70640.1"
                     /translation="MTLEHHRDLAHEFPELKQRFHDLKTESAGFRRLYAHYEALDNEI
                     HRIEQEIETPSDAYTEKLKLRRAQLKDRLYGLLTGRIHLPADTEEYVVRHKFRQPVDH
                     GEVSRAWSEQGYSCQSCTAAADPDWRDAAPGSDEVLVVGEGCLEVEMHGVRYALNPGD
                     EMFIPRAAPYRLRNACARETRWFHGHD"
     gene            complement(1208..2641)
                     /gene="pyk"
                     /locus_tag="PLW13_09445"
     CDS             complement(1208..2641)
                     /gene="pyk"
                     /locus_tag="PLW13_09445"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY70641.1"
                     /translation="MQRRTKIVATLGPASSSEGVIAALIDAGVDVFRLNFSHGQVDDH
                     RARAALVRTLAARAGREVALLGDLQGPKIRIRSFRDGAVELRNGAPFTLDCGLPDGEG
                     DESAVGVDLASLPGSVNPGDILILDDGRISLTVAAVHGQRVECTVRMGGRLSNRKGLN
                     RLGGGLSAPALTDRDCRDIEHAAELGLDYLAVSFPLTAADIEHARSLLQATGCQARII
                     AKIERAEVVHDERVLDAMILASDGIMVARGDLGVEVGDAELIGIQKQLIRKARRADRV
                     VITATQMMESMVQNPIPTRAEVFDVANAVLDGTDAVMLSAETASGKYPVETVTSMAST
                     CLGAETYPDVRRSQHRMDREFSRVDETIAMAAVYAANHLERVAGMVCLTESGTTALRM
                     SRLSSGLPIYALSRHPEIVRRMCLYRGVRPILFDYTVCAAGEVAQEAVNELLRRGLVG
                     SGERLILTRGDLLGVGGSTTTLKIVEL"
     gene            2801..4297
                     /locus_tag="PLW13_09450"
     CDS             2801..4297
                     /locus_tag="PLW13_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HQY70642.1"
                     /translation="MVELFRRAAAHGARIAFRSPAGETSYATLLARSASIAQALLAGR
                     ADLAEERIAFMLPAGADYALAQWGIWRAGGIALALNAAAAPEELEHMLRTAGIRRVIV
                     RAEAGAALAATCAALGVHCVGLDSLQGATDGTLPALGPERRAMIVFTSGTTSKPKGVV
                     STHGVIEAQIRTLVEAWEWQAGDCIPLFLPMHHVHGIVNVMSCALWSGACVEPFEGFQ
                     QEAVLGRVAAGAYSVFMAVPTIYVKLIQALEQMDAAQRDRLCAAFAGMRLMVSGSAAL
                     PANVHARWEQLTGQRLLERYGMTEIGMALSNPLHGERRPGSVGLPLPGVAVKLVTEAG
                     AEVSGEDEPGEIRVRGPGVFREYWNNPEATAKSFVDGWFCTGDVAVRERGYYRIMGRQ
                     SIDIIKSGGYKISALEIENVLLAHPAIRECAVFGIEDPTWGEIIAVAAVLREGAALGL
                     DALNAWCAGRLSAYKFPRRLAIVASLPRNAMGKVLKPALRPLFSGGSG"
     gene            complement(4254..5168)
                     /locus_tag="PLW13_09455"
     CDS             complement(4254..5168)
                     /locus_tag="PLW13_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQY70643.1"
                     /translation="MNASIRQLKAFVAVAQTRSFAQACGLLHLSQPALSIAIRNLEQA
                     VGGTLFVRTTRSIALSPEGEAFLPLAQRLLRDWDSAFGDLRELFGRQRGKLIVAAMPS
                     FASSLLPEVLLGFHRQYPNINISVQDVVAENVVAMVREGRAELGVSFAPHAPGELQFE
                     ALFLDRFVAVLPREHELLRQRSLRWRQLQPYPFIALQQPSAIREQIEQFLHEQSLQLP
                     VRMESHQLATIGRMVAAGLGLSVVPALCTGPMEEMGNVCRRLSGPNISRAVGILTRRH
                     QPLSAAAAAMRSALLTRFPPKKADAGRV"
     gene            5273..5980
                     /locus_tag="PLW13_09460"
     CDS             5273..5980
                     /locus_tag="PLW13_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQY70644.1"
                     /translation="MAGFDKVVQTYDEALAGLRDGMTVIAGGFGLCGIPEGLIAQIRR
                     MGVRDLTVVSNNCGVDGFGLGILLEDRQVRKMISSYVGENKLFEQQFLSGELEVELTP
                     QGTLAEKMRAGGAGIPAFFTATGYGTPVGEGKEVREFNGRPYILEEAITGDFAIVKAW
                     KADRYGNAIYRHTAQNFNPMAATAGRITVLEVEEIVEPGALEPSQIHTPGIYVNRVIR
                     GTFEKRIEQRTVRAKGG"
     gene            5985..6641
                     /locus_tag="PLW13_09465"
     CDS             5985..6641
                     /locus_tag="PLW13_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HQY70645.1"
                     /translation="MALTREQMAQRVARELQDGYYVNLGIGIPTLVANYIPEGVHVML
                     QSENGLLGMGPFPTEDEVDADMINAGKQTVTAITGAAIFSSAESFAMIRGGHVDLTVL
                     GAFEVDVNGDIASWMVPGKLVKGMGGAMDLVAAARNIIVIMTHADKAGNSKLLDACTL
                     PLTGKGCIRRVLTDLAWLEIDDGAFLLRERAPGVSVEEIRAKTAGKLVVPADVPEMRF
                     "
     gene            complement(6689..7690)
                     /locus_tag="PLW13_09470"
     CDS             complement(6689..7690)
                     /locus_tag="PLW13_09470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HQY70646.1"
                     /translation="MRAIVCSEYGPPSALVLRELPDPQPGPGQVLVRIHAAGVNFVDA
                     LLIGGSYQIRIPPPFVPGGDCAGTVIATGAGVTGFAPGDRVLASPGIGAYAELITLAG
                     AQLAHMPANMDFAGAAVFLQANATAYFALVNRGALKAAETLLVLGAAGGTGAAAVQVG
                     KALGARVIAAASSAEKLQACRALGADVLIDYAAGDLRAELKAATGGRGVDLVFDPVGG
                     ALAEPALRSCAENGRYLVIGFASGEIPKLPFNLPLLKSCQVVGVDWGGFAGRHPEGGR
                     AVTDAVLALYAQGRLGDPPLAHYPLERTAEAIADLAARRIAGKCVIDVDLDPSGHRP"
     gene            complement(7706..8914)
                     /locus_tag="PLW13_09475"
     CDS             complement(7706..8914)
                     /locus_tag="PLW13_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY70647.1"
                     /translation="MSLPAIELYDPDVYAAGVPHEQMRWLREHAPVYWHRHPDGGGFW
                     LVSRHADVVAVSRDHRSFSSERGFVMIDDLAADILADTRGQLLGMDPPRHGPIRRIVI
                     ERFTQRMLEQLEPRVRAITRGIFAAARAKPDCDFVYDIAGQLPTAVICTMLGVPEEMW
                     HQVREWSDKSTSASDPDIGCSPEEQREAGIAMGMYGYSLAAERKDRGGDDLISLLVNV
                     EVDGHKVSEAEFASLFLQITVAGNETTRALIATGMHELLQRPALYRELEAEPARLPGA
                     IEEMLRWSTPLHHFRRTATRDCEIAGQPVRENDRVVLLYTSANFDEAVFADPMRFDIR
                     RNPNPHLAFGHGIHLCLGANLARLEARVFFEEFFRHFSGIEATGEARRIRSNLVNGHK
                     YMPVRLHPRT"
     gene            complement(8922..9428)
                     /locus_tag="PLW13_09480"
     CDS             complement(8922..9428)
                     /locus_tag="PLW13_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HQY70648.1"
                     /translation="MASVELPTPVKITELPETPLTSGHINHNDLPWVEQGGGIEMKLL
                     RVSDDTGHWISMNRFQPGAQLPPHRHSGGVFAFTISGTWGYLESDFLSTAGSVVREPA
                     NTAHTLYVPADAKEPAVVIFFIEGSLVHYLPEGSIWGISDGHTQIADYIALAQAQGHP
                     VTRDMLLK"
     gene            complement(9504..11288)
                     /locus_tag="PLW13_09485"
     CDS             complement(9504..11288)
                     /locus_tag="PLW13_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70649.1"
                     /translation="MRLPAAALGICALAFCAGAGSIELTVRDSAGTPVASAMVTRSPA
                     GTTPADTSDNGYPAPGRPNLAPAQTTRFSDAAGKVRFEPTAELVNLRVRKPGYADSRV
                     QGIRGDEAPELLLAPQTDPLTLAESKPSNLWLSLLDFDGDARAREHFLLHCAFCHQQA
                     SPFMRRPRSGEEWRDAIDRMNRYGAQMAHEFRQPLARYLNARFEAIRDRPELLPDFEP
                     WEPHLAGVEITEWPIGNSLSQMHDFLPHPNGMVYVGDNLMDRIYELDPLSGEYRVYPI
                     PHAEGAPLGGILGNRFAAGYPKVDNYLGVHSFALSPVDGHIFITPSMAQSLLEFDPVS
                     KRFTEWKMDEGFYPHTIRVDAGDRVWFTLALSNQVAMFDRAREEFSYFDLPPRSLRER
                     VVSAVVKWRLGRGDVSEPPEYDWDNTGFPMPYGIDIAPDGTVWVARLYAGDIVRIDPA
                     SGETQAIPAPFTGPRRLRCDAAGNPWISGFSSGLIARYDVSQHRFDTWPLPVVSETPY
                     ALNVDRARNVVWVNGNQSDTLLRFDIASATWKVYPLARYRSFTRDIEIDEDGAVYTSV
                     SNFPAWQIEDAQPTLIRLREAGAKLASP"
     gene            11517..12008
                     /locus_tag="PLW13_09490"
     CDS             11517..12008
                     /locus_tag="PLW13_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQY70650.1"
                     /translation="MEQLTTIELAKEYMKFSAAHFTIFSATERERLHGHNFRVAASIT
                     APVGDNGMCFSYRILKDKLERLCEELDEYMLLPALSPHLRIGEQGAQYRVEFAGEELF
                     FPRSDTLLLPVRNSTVEEYARYLMQRLLEDSALIGGRDIREVGIKVSSGPGQWGTSHW
                     RRD"
     gene            12012..12713
                     /locus_tag="PLW13_09495"
     CDS             12012..12713
                     /locus_tag="PLW13_09495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70651.1"
                     /translation="MKTLVITGGSKGIGLATARLFAAEGYRVVNISRSPIALPGAVQL
                     GIDIAEPDWVARHAEAVRAAVAGSERIALVHNAGLLTKDSVANVDAAALHRVLQVNVV
                     ACVQLDQLLLPLMGAGSSIVYVGSTLGEKAVAGACAYVVSKHALIGLMRATCQDLAGR
                     GIHTACVCPGFTDTEMLRAHVGDAFEVLEALAGNVTFGRLIEPGEVARTIHFCAHNAV
                     INGAVIHANLGQVER"
BASE COUNT         2001 a         4304 c         4611 g         2017 t
ORIGIN      
        1 gcacattcct actacgcgat gtgggccgtg agcgcggaca agccgtagac cggcattgga
       61 tcgtgcgtcg gcatgaatgc cgacctgcac gatgagggtc cggccacgcc ggacccgcca
      121 ccaccccgtc attgacgtcg attgcccgtc accgccgcgt ttgtggcctg tttgggtcat
      181 gacgggcacg cacccatctg gtatcaagag cgcgtcgccg ccgaccccga ggtcgatcga
      241 gactggtgca atgactcgct aattcgactg gggccgcgaa cggacccggg agaccgaccg
      301 atgcccacga atgacaccga tcatcgcctg tttggcgaat acctgcactg gattgcgcac
      361 aaggaactgg cgctcggcgc ggttcgcgcc gagctctggg ccagcgcggt ggtccagtgc
      421 gcgggcgacg aagacaaggc gcggggcata tacctcgagt cgcgggcgca gtcgctgcgc
      481 gaagaggcat cgattgcgga gaaattcctg cgcctgctcc gccagagcga ggacagcgcc
      541 gtgcaggtgc acgacgccgt ggttttttac ctcgcgcggc ccgccgcggg gcgcgtcgaa
      601 tctccgcgat aaggccccgg ccgctcaatc gtgaccatga aaccagcgcg tttcccgcgc
      661 gcaggcattg cgtaagcggt atggcgccgc gcgcgggatg aacatttcgt cgcccgggtt
      721 cagggcgtaa cgcacgccgt gcatttccac ctccaggcag ccctcgccga ccacgagcac
      781 ctcgtcgctg ccgggcgccg cgtcgcgcca gtccggatcc gcagctgcgg tgcagctctg
      841 gcaactgtag ccctgttcgc tccaggcgcg tgacacttcg ccgtgatcca ccggctggcg
      901 gaacttgtgg cgtaccacgt actcctcggt gtctgccggc aggtggatgc ggccggtcag
      961 caggccatac aggcgatcct tcagctgcgc gcgccgaagc ttcagcttct cggtgtaggc
     1021 atcggagggc gtctcgatct cctgctcgat gcggtggatc tcgttgtcca gcgcctcgta
     1081 gtgcgcatac aggcggcgga acccggcgct ttcggtcttg aggtcgtgga aacgctgttt
     1141 cagttcgggg aattcgtgcg cgaggtcgcg gtggtgctcg agagtcatgg gtcggtctct
     1201 tccggattca aagttccaca attttcagcg tggtcgtcga gccgccgacg ccgagcaggt
     1261 cgccgcgcgt caggatgagc cgctcgccgc tgccgaccag tccgcggcgc agcagttcgt
     1321 tcactgcctc ctgcgccacc tcgccggccg cgcaaaccgt gtagtcaaac aggatcgggc
     1381 gcacgccgcg atacaggcac atgcggcgca cgatctccgg gtggcggctc agcgcgtaga
     1441 tcggcaagcc ggagctgagg cgcgacatac gcagcgcggt cgtgcccgat tcggtcaggc
     1501 acaccatgcc ggcgacgcgt tccagatgat tcgcggcgta gacggcggcc atcgcgatgg
     1561 tctcgtcgac gcgcgagaat tcgcggtcca tgcggtgctg cgagcggcgc acgtcggggt
     1621 aggtctcggc cccgaggcag gtcgaggcca tcgatgtcac ggtctccacc gggtacttgc
     1681 cgctggcggt ctccgccgag agcatcaccg cgtcggtgcc gtcgagcacg gcgttggcga
     1741 cgtcgaacac ctcggcgcgc gtcgggatcg ggttttgcac catcgattcc atcatctgcg
     1801 tggcggtgat caccacgcgg tcggcgcgcc gcgccttgcg gatcaactgc ttctggatgc
     1861 cgatcagctc ggcgtcgcct acctccacgc cgaggtcgcc gcgcgcgacc atgatgccgt
     1921 cggaggcgag gatcatcgca tccagcaccc gctcgtcgtg cacgacctcg gcgcgctcga
     1981 tcttcgcgat gatgcgcgcc tggcagccgg tggcctgcag caggctgcgc gcgtgctcga
     2041 tgtcggccgc ggtcagcggg aaggacaccg cgaggtagtc gaggccgagt tcggccgcgt
     2101 gctcgatgtc cctgcagtcg cgatcggtca gcgcgggcgc cgacagcccg ccgccgaggc
     2161 ggttgagtcc cttgcggttc gacagccgcc cgcccatgcg caccgtgcat tccacgcgct
     2221 gcccgtgcac cgcggccacg gtgaggctga tgcggccgtc gtcgaggatc aggatgtcgc
     2281 cgggattgac actcccgggc aggctcgcga ggtcgacacc cacggcactc tcgtcgccct
     2341 cgccgtcggg caacccgcag tcgagcgtga agggtgcgcc gttgcgcagt tcgaccgcgc
     2401 cgtcgcgaaa gctgcggatg cggatcttcg gtccctgcag gtcgccgagc agcgcgacct
     2461 cgcggcccgc gcgtgccgcc agggtgcgca ccagcgcggc gcgagcgcgg tgatcatcga
     2521 cctggccgtg cgagaagttg aggcggaata cgtccacgcc ggcgtcgatc agcgccgcga
     2581 tgaccccttc agaactgctg gccggcccca gcgtggccac gatcttggtg cgtcgctgca
     2641 cgcccatgca aaactccctg gcgatggtgt gcgcgctcgc atccggcgag ggcgcggcgg
     2701 cgattatgcc ccagtcacgg cccgcggtgg catcgtccgg ttgacggcgt gcgtggcgct
     2761 tcggtatacc ggcgcctcct ttgcgcatcg aggtgccccg atggtcgagc tgtttcgtcg
     2821 cgccgcggca cacggtgcgc gcatcgcgtt tcgctccccc gcgggcgaaa cctcctacgc
     2881 gacgctgctg gcgcgctcgg cgtccatcgc gcaggcgctg ctcgccgggc gcgcggacct
     2941 ggcggaggag cgcattgcct tcatgctgcc cgcgggagcg gattacgcgc tcgcgcagtg
     3001 gggcatctgg cgcgccggcg gcatcgcgct ggcgctgaac gccgcggcgg cacccgagga
     3061 gctcgagcac atgctgcgca cagcgggcat acggcgcgtg atcgtgcgcg ccgaagccgg
     3121 ggcggcgctc gcggcgacct gcgccgcgct cggcgtgcac tgcgtagggc tggactcgtt
     3181 gcagggggca accgacggca cgctgccggc gctggggccc gagcggcgcg cgatgatcgt
     3241 gttcaccagc ggcaccacca gcaagcccaa gggcgtggtg tccacgcacg gcgtgatcga
     3301 ggcgcagatc cgcacgctgg tcgaggcctg ggagtggcag gccggggact gcatcccgct
     3361 gttcctgccg atgcaccacg tccacggcat cgtcaatgtg atgagctgtg cgctgtggag
     3421 cggagcctgc gtggagccgt tcgagggttt ccagcaggag gcggtgctcg ggcgcgtcgc
     3481 ggcgggtgcc tacagcgtgt tcatggccgt gccgaccatc tacgtgaagc tgatccaggc
     3541 gctggagcag atggatgcgg cgcagcgcga tcggctgtgc gccgcgttcg cgggcatgcg
     3601 cctgatggtg tcgggctcgg cggcgctgcc ggcgaacgtg cacgcacgct gggagcaact
     3661 caccgggcag cgcctgctcg agcgctacgg catgaccgag atcggcatgg cactgtctaa
     3721 cccgctgcac ggcgagcgcc gtccgggcag cgtggggctg ccgctgcccg gcgtggcggt
     3781 gaagctggtc acggaagcgg gtgccgaagt gtccggggag gacgagccgg gcgagatccg
     3841 ggtgcgcggc cccggcgtgt tccgcgagta ctggaacaac cccgaggcca cggcgaagag
     3901 cttcgtcgac ggctggttct gcaccggcga cgtggcggtg cgcgagcgcg gctactaccg
     3961 catcatgggg cggcagtcga tcgacatcat caagtccggg ggctacaaga tctcggcgct
     4021 ggagatcgag aacgtgctgc tcgcgcaccc ggcgatccgc gaatgcgcgg tattcgggat
     4081 cgaggatccg acctggggcg agatcatcgc ggtggccgcg gtgttgcgcg agggtgcggc
     4141 gctcggcctc gatgcgctga atgcctggtg cgccggcagg ctgtcggcct acaagttccc
     4201 gcgtcgcctc gccatcgtcg cctcgctgcc gcgcaacgcc atgggcaagg tgctcaaacc
     4261 cgccctgcgt ccgctttttt cggggggaag cgggtgagga gggcgctgcg catggccgcg
     4321 gcggcggcgg acaggggttg gtggcggcgg gtgagtatgc cgacggcgcg cgagatgttc
     4381 ggcccgctca ggcgccggca cacattgccc atttcttcca tcgggccggt gcacagcgcc
     4441 ggcaccacgc tcaggccgag cccggcggcg accatgcgcc cgatggtcgc gagctggtgg
     4501 ctctccatgc gcaccggcag ctgcagcgac tgctcgtgca ggaactgctc gatctgctcg
     4561 cggatggccg agggctgctg cagcgcgatg aacgggtagg gctgcagctg gcgccagcgc
     4621 aggctgcgct ggcgcagcag ttcgtgctcg cgcggcagca ccgcgacgaa gcgatccagg
     4681 aacagcgcct cgaactgcaa ctcgccgggc gcatgcggcg caaagctgac gccgagctcg
     4741 gcgcgcccct cgcgcaccat cgccacgaca ttctccgcca ccacgtcctg cacgctgatg
     4801 ttgatgttcg ggtactggcg atggaagccg agcagcacct cgggcagcag gctcgaagcg
     4861 aaggacggca tcgccgcgac gatcagcttg ccccgttgcc tgccgaacag ttcgcgcaaa
     4921 tcgccgaagg cgctgtccca gtcgcgcagc aggcgttgcg cgagcggcag gaaggcctcg
     4981 ccctcgggcg acagcgcgat cgagcgcgtg gtgcgcacga acagcgtgcc gccgaccgcc
     5041 tgctcgagat tgcggatcgc gatgctgagc gcgggctgcg acaggtgcag caggccgcag
     5101 gcttgcgcga agctgcgcgt ctgcgccacg gccacgaagg ccttcagctg ccggatgctt
     5161 gcattcattt ggaataatga tgaatcgcta gaaagaatca attggctaaa tataaggagc
     5221 tgcggcacga tcctcaacgc cgatcggccg gcaagcgcaa ggagcagtga acatggcagg
     5281 tttcgacaag gtggtgcaga cctacgacga ggcgctcgcg ggcctgcgcg acggcatgac
     5341 cgtgattgcc ggaggcttcg gcctgtgcgg catacccgag gggctgatcg cacagatccg
     5401 ccgcatgggc gtgcgcgatc tcacggtggt ctcgaacaac tgcggtgtcg acggtttcgg
     5461 gctcggcatc ctgctcgagg accgtcaggt ccgcaagatg atttcctcct acgtcggcga
     5521 gaacaagctg ttcgagcagc agttcctgag cggcgagctc gaggtcgagc tcacgccgca
     5581 gggcacgctc gccgagaaga tgcgcgccgg cggcgccggc atcccggcgt tcttcaccgc
     5641 caccggctac ggcaccccgg tgggcgaggg caaggaagtg cgcgagttca acgggcggcc
     5701 gtacatcctc gaggaggcga tcaccggcga cttcgcgatc gtgaaggcgt ggaaggccga
     5761 ccggtacggc aacgcgatct accgtcacac ggcgcagaac ttcaacccga tggcggccac
     5821 cgccgggcgc atcaccgtgc tcgaggtcga ggagatcgtc gagcccggcg cgctggagcc
     5881 cagccagatc cacacgcccg gcatctacgt caaccgcgtt atccggggca ctttcgagaa
     5941 acgcatcgag cagcgcaccg tgcgcgccaa gggaggctga aacgatggca ctcacccgcg
     6001 aacagatggc gcagcgcgtc gcgcgcgaac tgcaggacgg ttactacgtg aacctcggca
     6061 tcggcatccc gacgctcgtg gcgaactaca tccccgaggg cgtgcacgtg atgctgcagt
     6121 ccgagaacgg cctgctcggc atgggcccgt tcccgaccga ggacgaggtc gacgcggaca
     6181 tgatcaacgc gggcaaacag accgtcaccg cgatcacggg cgcggcgatc ttctcctcgg
     6241 cggaatcctt cgcgatgatc cgcggcggtc acgtggacct cacggtgctc ggcgccttcg
     6301 aggtggacgt gaatggcgat attgcctcgt ggatggtgcc gggcaagctg gtgaagggca
     6361 tgggtggcgc gatggatctc gtggccgcgg cgcgcaacat catcgtgatc atgacgcacg
     6421 cggacaaggc cggtaactcg aagctgctcg atgcgtgcac cctgccgctg accggcaagg
     6481 gctgcatccg ccgggtgctc accgatctcg cctggctcga gatcgatgac ggggccttct
     6541 tgctgcgcga gcgcgcgccc ggagtcagcg tcgaggagat ccgcgcgaag acggcaggca
     6601 aactggtcgt gccagcggat gttcccgaga tgcgcttctg attccgtggg tccggcttca
     6661 gccggacaat gtccggatga atccggacct acggtcgatg tccggatgga tcgaggtcca
     6721 cgtcgatgac gcacttgccc gcgatgcgcc gcgccgcgag gtcggcgatg gcctcggcgg
     6781 tgcgctccag cgggtagtgc gccagcggcg gatcgccgag ccggccctgc gcatacagcg
     6841 cgagcactgc atcggtcacc gcgcggccgc cctcgggatg gcgtcccgcg aagccgcccc
     6901 agtccacccc caccacctgg cagctcttca gcagcggcag gttgaacggc agtttcggaa
     6961 tctcgccgct ggcgaagccg attaccaggt agcgcccgtt ctcggcgcag gaacgcagcg
     7021 ccggctcggc cagcgcgccg ccgaccgggt cgaacacgag gtccacgccg cgcccgccgg
     7081 tggcggcctt gagctcggcg cgcaggtccc cggcggcgta atcgatcagc acatcggcgc
     7141 cgagcgcgcg gcaggcctgg agcttctccg cgctcgaggc ggcggcgatc acccgtgcgc
     7201 ccagcgcctt gcccacctgc accgcggcgg cgccggtgcc gccggccgcg cccagcacca
     7261 gcagcgtctc tgcggctttc aacgcgccgc gattcaccag cgcgaaatac gccgtggcat
     7321 tggcctgcag gaacaccgcc gcgcccgcga aatccatgtt cgcgggcatg tgcgcgagct
     7381 gcgcgcccgc cagcgtgatc agctcggcgt aggcgccgat gccggggctc gcgagcactc
     7441 ggtcgcccgg cgcgaaaccc gtgacgccgg cgccggtcgc gatcaccgtg ccggcgcaat
     7501 cgccgccggg cacgaagggc ggcgggatgc ggatctggta gctgccgccg atcagcagcg
     7561 catcgacgaa gttcacgccg gcagcgtgga tgcgcaccag cacctgcccg ggtcccggct
     7621 gcgggtcggg caactcgcgg agtaccaggg cggaaggcgg cccgtattcg ctgcaaacga
     7681 tggcgcgcat ggcgtgatgt tcccttcagg tgcgcgggtg caggcgcacc ggcatgtact
     7741 tgtgcccgtt caccaggttg gaacggatgc gccgcgcctc gccggtggcc tcgatgccgc
     7801 tgaaatgacg aaagaactcc tcgaagaaca cccgcgcttc cagccgcgcc aggttcgcgc
     7861 ccaggcacag gtggatgccg tggccgaagg cgaggtgcgg gttcgggttg cggcggatgt
     7921 cgaagcgcat cggatcggcg aacaccgcct cgtcgaaatt ggccgaggtg tacagcagca
     7981 ccacgcggtc attctcgcgc accggctggc ccgcgatctc gcaatcacgc gtggcggtgc
     8041 ggcggaagtg gtgcagcggc gtgctccagc gcagcatctc ctcgatggcc cctggcagcc
     8101 gtgccggctc ggcctcgagc tcgcggtaca gtgcggggcg ctgcagcagt tcgtgcatgc
     8161 cggtggcgat cagggcgcgc gtggtctcgt tgccggccac cgtgatctgc aggaacagcg
     8221 aggcgaactc cgcctcgctg accttgtgcc cgtcgacctc cacgttcacg agcagcgaga
     8281 tcaggtcgtc gccgccgcgg tccttgcgct cggccgccag cgagtagccg tacatgccca
     8341 tcgcgatccc ggcctcgcgc tgctcctcgg ggctgcagcc gatgtccggg tccgaagcgg
     8401 aggtgctctt gtccgaccac tcgcgcacct ggtgccacat ctcctcgggc actccgagca
     8461 tggtgcagat caccgcggtg ggcagctggc cggcaatgtc gtagacgaag tcgcagtcgg
     8521 gtttcgcgcg cgccgcggcg aagatgccgc gcgtgatggc gcgcacccgc ggctcgagct
     8581 gctcgagcat gcgctgcgtg aagcgctcga tcacgatgcg gcggatcggg ccatggcgcg
     8641 gcggatccat gccgagcagc tgtccgcgcg tgtcggcgag gatgtcggcc gcgagatcgt
     8701 cgatcatcac gaaaccgcgc tcggaggaaa acgaacggtg gtcgcgcgac accgcgacca
     8761 cgtcggcatg gcggctcacc agccagaagc cgccgccgtc cgggtgacgg tgccagtaca
     8821 ccggcgcgtg ctcgcgcagc caccgcattt gctcgtgcgg cacgcctgcg gcgtagacgt
     8881 cgggatcgta gagctcgatg gcgggcaggc tcatgcgcgc ctcacttcag cagcatgtcg
     8941 cgcgtgaccg ggtgcccctg cgcctgcgcc agcgcgatgt agtcggcgat ctgcgtgtgt
     9001 ccgtccgata tgccccagat gctgccttcg ggcaggtagt gcaccaggct gccctcgatg
     9061 aagaagatca ccacggccgg ctccttcgca tcggcgggca catacagcgt gtgcgcggtg
     9121 ttggcgggct cgcgcaccac cgatcccgcg gtggagagaa agtcgctctc gaggtagccc
     9181 caggtccccg agatcgtgaa cgcgaacacg ccgccgctgt ggcggtgcgg cggcagctgc
     9241 gcgccgggct ggaagcggtt catgctgatc cagtggccgg tatcgtcgga tacccgcagc
     9301 agcttcatct cgatgccccc gccttgctcc acccacggca ggtcgttgtg gttgatatgt
     9361 cccgaggtga ggggggtctc ggggagttcg gtgatcttta ccggtgtcgg gagctctacg
     9421 cttgccatga ggggactctt cgcgcggtgg aaagggagcc gcaatctacg cccggccccg
     9481 gcgtggagta aagcgcgcgg gcattacgga ctcgcaagct tggcgcctgc ctcgcgcaaa
     9541 cggatcaggg tcggctgcgc gtcctcgatc tgccacgccg ggaagttcga cacactggtg
     9601 tagaccgcgc cgtcctcgtc gatctcgatg tcgcgcgtga acgagcggta gcgcgcgagc
     9661 ggatagactt tccaggtcgc gctcgcgatg tcgaaacgca gcagcgtgtc ggactggttg
     9721 ccattcaccc acaccacgtt gcgcgcgcgg tcgacgttca gcgcataggg cgtttcgctg
     9781 acgaccggca gcggccaggt gtcgaagcga tgctgcgaga cgtcgtagcg cgcgatgagg
     9841 cccgaggaga aacccgagat ccacgggttg ccggcggcgt cgcagcgcag gcggcgtggc
     9901 ccggtgaagg gggcgggaat ggcctgcgtc tcgcccgaag ccggatcgat gcgcacgatg
     9961 tcgccggcat agagccgcgc cacccagacc gtgccgtcag gtgcgatgtc gatgccgtag
    10021 ggcatcggga agccggtgtt gtcccagtca tactcgggcg gctcgctgac gtcgccgcgc
    10081 cccaggcgcc acttcaccac ggcgctcacc acgcgctcgc gcaggctgcg tggcggcagg
    10141 tcgaagtagc tgaactcctc cctcgcgcgg tcgaacatcg ccacctggtt gctcaatgcc
    10201 agcgtgaacc agacgcgatc cccggcatcg acgcggatgg tgtgcggata gaagccctcg
    10261 tccatcttcc attcggtgaa gcgcttgctc accgggtcga attcgagcag cgactgtgcc
    10321 atcgagggcg ttatgaagat gtgcccgtcg acgggcgaca gcgcgaagga gtgcacgccc
    10381 aggtagttat cgaccttggg gtagccggcg gcgaaacggt tgccgaggat gccgccgagc
    10441 ggcgcgcctt cggcgtgcgg gatggggtag acgcggtatt cgcccgacag cgggtcgagt
    10501 tcgtagatgc ggtccatcag gttgtcgccg acgtagacca tgccgttggg gtggggcagg
    10561 aagtcgtgca tctgcgagag cgagttgccg atgggccact cggtgatctc gacgccggcg
    10621 aggtgcggct cccacggctc gaagtccggc agcagctcgg ggcggtcgcg gatcgcctcg
    10681 aagcgcgcgt tcaggtagcg cgccagcggc tggcggaatt cgtgcgccat ctgcgcgccg
    10741 tagcggttca tgcggtcgat ggcgtcgcgc cattcctcgc ccgagcgggg cctgcgcatg
    10801 aacggcgacg cctgctggtg gcagaacgcg cagtgcagca ggaagtgctc gcgcgcgcgg
    10861 gcatcgccgt cgaagtcgag cagcgagagc cacaggttcg acggcttgga ttcggccagc
    10921 gtgagaggat cggtttgcgg cgcgagcagc agctccggcg cctcgtcgcc gcggatgccc
    10981 tgcacgcgcg agtcggcgta gcccggcttg cgcacgcgca ggttcaccag ctcggcggtg
    11041 ggctcgaaac gcaccttgcc ggccgcgtcg ctgaagcgcg tggtctgcgc cggcgccagg
    11101 ttcgggcgcc ccggcgccgg gtagccgttg tcggaggtat cggcgggtgt ggtaccggcg
    11161 ggactgcgcg tgaccatcgc gctggcgacc ggcgtacccg cgctgtcgcg cacggtgagc
    11221 tcgatggacc cggcgcccgc gcagaaggca agggcgcaga ttcccagggc cgcggctggc
    11281 aggcgcatgc gtcggtctcc cgttggctgg cgcgccgatg gtgaccgttc ggcgcgcctc
    11341 atgcaaaccg gcgcccgcgc gcggaccggg cccggcgcgc ggggcgccgg gtcgaaacca
    11401 cgccccggca ggtccatgca aggcgcagga acggcgttcc acgtcgggcc acgccacgcc
    11461 ggcgcggtct cggctatagt gtcggctcgg gcaggcagca gcgggagtac accggcttgg
    11521 aacaactgac cacgatcgaa ctggcgaagg agtacatgaa gttctctgcc gcgcatttca
    11581 cgattttctc cgccacggag cgcgaacgcc tgcacggcca taacttcagg gtggcggcca
    11641 gcatcaccgc gccggtgggc gacaacggca tgtgcttcag ctaccgcatc ctcaaggaca
    11701 agctcgagcg gctgtgcgag gagctcgacg agtacatgct gctgccggcg ctctcgccgc
    11761 acctgcgcat cggcgagcag ggcgcgcagt accgcgtgga gttcgccggc gaggagctgt
    11821 tcttcccgcg cagcgacacg ctgctgctgc cggtgcgcaa ctccacggtc gaggagtacg
    11881 cgcgctacct gatgcagcgg ctgctcgagg acagcgcgct gatcggcgga cgcgatatcc
    11941 gcgaggtcgg catcaaggtt tcctccggcc ccgggcagtg gggcaccagc cactggcgca
    12001 gggactgagc aatgaagaca ctggtcatca cgggcggcag caagggcata gggctggcca
    12061 cggcgcggct gttcgcggcc gagggctacc gggtcgtcaa catctcgcgc agccccatcg
    12121 cgctgccggg cgcggtgcaa ctcggcatcg acatcgccga acccgactgg gtggcgcgcc
    12181 acgcagaggc cgtgcgcgcc gcggtggccg gcagcgagag gatcgcgctg gttcacaacg
    12241 ccggcctgct gaccaaggac agcgtcgcga acgtggatgc ggcggcgctg catcgcgtgc
    12301 tgcaggtcaa cgtggtcgcc tgcgtgcagc tcgaccagct gctgctgccg ctgatgggcg
    12361 ccggctcctc gatcgtctac gtgggctcga cgctgggcga gaaggcggtg gcgggcgcct
    12421 gtgcctacgt ggtgtccaag catgccctga tcggcctgat gcgcgcgacc tgccaggatc
    12481 tcgccgggcg cggcatccac accgcctgcg tctgtccggg cttcaccgat accgagatgc
    12541 tgcgcgccca cgtcggcgat gcgttcgagg tgctggaggc gctggccggc aacgtgacct
    12601 tcgggcgcct gatcgagccg ggggaggtcg cgcgcaccat ccacttctgt gcacacaacg
    12661 cggtgatcaa cggcgccgtc atccacgcca acctggggca ggtcgagcgc tgagcgctcg
    12721 ctgcgccgcg gcgggctgtt gtagccggcc gcatgctcgg ctagagtcgt ggcgccgccg
    12781 cgtggcggcg cgaacaaaca tactggagaa cacgaccatg atgcgtctgc cgatggcgac
    12841 cctgaccctc agtctcagcc ttttcgccgt cgctgcgacg gcggaatgca gccgtgaagc
    12901 cgcaccggcg atacccgacg gcaccgtggc gac
//
LOCUS       DAORUQ010000094        12824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4861_length_12824_cov_6.726212, whole genome shotgun sequence.
ACCESSION   DAORUQ010000094 DAORUQ010000000
VERSION     DAORUQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_12824_cov_6.726212"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLW13_09500"
     CDS             complement(<1..1527)
                     /locus_tag="PLW13_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HQY70652.1"
                     /translation="MDIKKLTPALSVAGQIAAADLPEIAGAGFRSIICNRTDGEAADQ
                     PGFSEIERAARSAGLEVRYLPAESGKVSDEQGAEFGRMMEELPRPVLAYCRSGMRSAT
                     MWALSQSGKIPLPEIIETGAKAGYDLRGLVRRIMNGGRTPVEVADASHEIVIIGGGAA
                     GVAVASSLLARRPGLDIAIIDPADVHYYQPGWTLVGAGVFKPAFTARTMAAVLPRGVH
                     WIKSAVAAFEPERNAVILDGCRVVSYRQLVVCPGIKLDWQGIEGLPETLGRNGVTSNY
                     RYDLAPYTWQLVQNLRGGRAVFTQPPMPIKCAGAPQKAMYLSADHWRRAGVLSQLDIR
                     FCNAGAVLFGVPDYVPALMEYVGAYGIGLEFNRNLVAVDGPARKATFRQTNADGSQQT
                     VNLDFDMIHVVPPQKAPDFIRVSPLADAAGWVDVDPATLRHRGFDNVFALGDAGNTTN
                     AKTAAAARKQAPVVAHNLLVTRGEARGDARYDGYGSCPLTVERGKIVLAEFTYGGKLA
                     P"
     gene            1816..3513
                     /locus_tag="PLW13_09505"
     CDS             1816..3513
                     /locus_tag="PLW13_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY70653.1"
                     /translation="MIRNLPAKWDLEADVVAVGSGGGGLAAAITAHDHGASALVLERC
                     DQVGGVTAYSMGEVWIPGNHLEAALGIEDSPESGFAYIKRLSMGYGDDLAILNQMVHG
                     PVALRFFEERIGLKMCVVRDLPDYYYPHVEASLAEGRCLEALPFPAQTLGDWQSRTRL
                     SPHVPYGMTHEDIFHNGGMANMMKWDYSVMAERLAKDERCAGPGLAAWFVKGVLDRGI
                     AMHTGVDVQELVVDGGRVVGLRARVDGRDLFVKANRGVVIAVSSYERNPGYAKTLGHQ
                     LDPVSMVMPTIDGAHLRLAGQAGARIARVPDVSMLGFHVPGEEQEDEVALWRGALPFM
                     GLPHTIVVNRAGKRFANEAFYRSVYFALGAIDGATQTHPNYPCWAIFDSQAREKYPFG
                     SVMPGEELPEAMGVTAHSIAELAARIGVDAHGLADTVARFNDYSARGEDPEFARGTFP
                     WGALMCGDPLQKPNANLGPLLKAPFHAVRLHQMAGGGIAATGILADHHCRAIGWDDEP
                     IEGLYVAGNSVARLDNGALMQSGITNARGMTHGYLAGRHAAGHPSALLQQERLRLGL"
     gene            3522..4064
                     /locus_tag="PLW13_09510"
     CDS             3522..4064
                     /locus_tag="PLW13_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY70654.1"
                     /translation="MIEAQNSIVIGAPIGNVWDYVQDISRWANLFPGCRECTVIDDHN
                     SRWLIKVGAGGLVRTVNVMVRVERWDGPERVDFSYALEAEPVVGGGSYVAAMKEPHET
                     EITLKVRVEGSGSMAPMWEAVSRPLLPQLAKSFSGRLKTEIENGATAALATASATPAR
                     PAPLRLIATWLRRTWQKLFG"
     gene            4043..4735
                     /locus_tag="PLW13_09515"
     CDS             4043..4735
                     /locus_tag="PLW13_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70655.1"
                     /translation="MAKAVRIDATVPSRRAAPVTRIRSNRARGRPSPEDAAAIDHELL
                     DIALGLFLQHGYGGTSMRQIVAAAGVSKTTLYSRFTSKADLFRAIMIQQIDRLSPSAS
                     LQSEGGPLALEAGLRSYANQMLAVSLEGDVLGVNRLIYSESHRFPELGAAAAERTALG
                     IARISDFVRTCARNAGRSCSDPKGIAEAFIFMIRGWYVNVLLTNREVPVAERERWVKR
                     AVRALVCGQHEW"
     gene            complement(4787..5839)
                     /locus_tag="PLW13_09520"
     CDS             complement(4787..5839)
                     /locus_tag="PLW13_09520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQY70656.1"
                     /translation="MIRTDDLRITQTQPLITPAVLCEELPHSDAGAVLVNSSRRTIEA
                     ILRGADRRLLCIVGPCSVHDPAAALDYARQMQLRTAGLGDALFIVLRVYFEKPRTVVG
                     WKGLINDPDLDGSFHINKGLHLARRLLLDVTALGLPAATEFLDTTFGQYYADLVSFGA
                     IGARTVESQVHRELASGLSMPVGIKNRTDGDVGVAIDAIRAAAHPHWFPSITKEGSPA
                     ILRSSGNPDCCLVLRGGNVSGPNFGAAAVNDALQRLQDAGLEAGLVVDCSHGNSGKDP
                     ARQADVAADLASQIRAGQRGIRGVMIESHLQGGAQKLGPRDTLRYGQSVTDACLALDD
                     TLALLEGLAGALRAAR"
     gene            complement(5836..6927)
                     /locus_tag="PLW13_09525"
     CDS             complement(5836..6927)
                     /locus_tag="PLW13_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY70657.1"
                     /translation="METDGNEQQRELARATDVAIRLGALALLIGWCALIVAPFLLAIA
                     WGCIIAIALHGPFLVLSQWLGGRRVLAATLLVLALLALIIVPGVMLTGTVVDDVQAVM
                     GAFQEGKLKVPPPSDKVAAWPIVGERVHALWLQASQNLESVFATLGPQMKPVAGWFLS
                     SAAQFGLGLLQFVFAIAISGALLASATTGGNVARALATRLVGERGAEFTTLAAATVRG
                     VTRGILGVALIQALLAGAALFLAGVPAAGFWTALALVLGVVQLGVGIIMVPAAIWLFS
                     TADLFTAAAFLAWTLLLMPLDNVLKPLLMGRGLNAPVAVIFVGAIGGFITQGIIGLFV
                     GAVVLVLGNELLRAWIAPQAAAAPGSTEP"
     gene            complement(6959..7546)
                     /gene="smrA"
                     /locus_tag="PLW13_09530"
     CDS             complement(6959..7546)
                     /gene="smrA"
                     /locus_tag="PLW13_09530"
                     /inference="COORDINATES: protein motif:HMM:NF033154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HQY70658.1"
                     /translation="MQKNTSEDEDFRELMSDVRPLAGDVQLHLKRAPEITPGTLARRL
                     AAESLVRKDGNFLSDSHIPLVEPHAELSFIRPGVQYGVFKRLRLGDYRFDSRLDLHGL
                     TVEQSREALWRFVADCMKHDVRAALINHGKGAGRSTPALLKSCVAMWLPQFAEVLAFH
                     SAPRHLGGTGATVILLRKSERARQENFEKHSRRAR"
     gene            7758..9383
                     /locus_tag="PLW13_09535"
     CDS             7758..9383
                     /locus_tag="PLW13_09535"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HQY70659.1"
                     /translation="MLGLIMNRQLTITSILEHARKFHGDAEVVSVTADQPLHRYTYRE
                     AFARSARLANALARLGIRPGERIGTLAWNDYRHLEVYFATSCAGFVCHTINPRLFPEQ
                     IEYIVEHAEDRWIFVDPAFVPLLEALKHRLGCVEGFVILGPAGAAANTTLRNAVDYET
                     LIAGEPDTFDWPALDENSACAMCYTSGTTGNPKGVVYSHRALVLHTYACLMPDVMSLS
                     AREVVMPVVPMFHVNAWSIPYGAAAVGAKLVFPGPKMGDGETLQRLIEGEGVTVTAGV
                     PTVWLALLTHLDRTGKQVPSLRRVIVGGSACPRTIMEEFEHKHGVHVHHAWGMTEMSP
                     MGTFNSPSADFDTLSSEEQWAQRLRVGRPVYGVEVKIVDAAGKELPWDGVSFGALKVR
                     GPWICAEYFRIGKGDAHDDEGWFETGDVAAIDRRGYVAITDRTKDVIKSGGEWISSID
                     LENTAVAHPAVAEAAVIGAYHPRWDERPLLCVVLHPGEQLTREDMLAWFEGKVAKWWV
                     PDDVVFVDGLPHTATGKLSKKDLRERMKDYRFP"
     gene            9508..11286
                     /locus_tag="PLW13_09540"
     CDS             9508..11286
                     /locus_tag="PLW13_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY70660.1"
                     /translation="MSDYKAPVTEIAFVLDEHAGMAGIAALPGLEEATPDVVQAVLEG
                     VATLTEEVIAPLNWPGDRQGTRIENRAVVQADGFKEAYWQYVEGGWGSLPFPAELGGQ
                     GMPVVLATAVLEMVQSGGIAFGLCPLLTQGATDAILAHGSDELKRTYLPKMISGEWTG
                     TMNLTEPQAGSDLAAVAARAQRDGDHYRIFGNKIFITWGDHGMTENVIHLVLARLPDA
                     PPGVRGISLFLVPKFLVNADGSPGARNDVYPVSVEHKLGIHASPTCVMGFGDDGGAIG
                     YLVGEEGKGLACMFTMMNHARLSVGLQGVAVSERSLQRAVAFARERVQGAVAGQPGRA
                     PIVHHPDVRRMLLQMKALTEAGRALAYVAMGSQDRIHHSPDAAERAFHEERIALLTPI
                     VKGWCTEMSMEITSLGVQVHGGMGFIEETGAAQHLRDARILPIYEGTNGIQALDLVGR
                     KFLRDGGAGMKSLLAEMRGVATALGAGALADIGTRLARAIDALQDAADWIRANADAAG
                     VVPGAAAYNFLMLAGTVAGGWQSGVAALAAQRRLAAGADNADFLRAKLLTARFYAEQI
                     LPRAQMHAEAVQAGAQTIMAFDEAML"
     gene            11368..12798
                     /locus_tag="PLW13_09545"
     CDS             11368..12798
                     /locus_tag="PLW13_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY70661.1"
                     /translation="MQRCEQFYINGQWVDPVERRTLEVINPATEQPIATIALGSAADV
                     DKACTAARAAFPAYSRTSRAERIALLERIIAGYQARYGEFAAVISQEMGAPITLATRA
                     QAAMIVGHFSAALQVLKEFRFEEDIGTTRVLREPIGVCAFITPWNWPANQIACKVAPA
                     LATGCTMVLKPSEIAPLDAYLFAQVLHDAGVPPGVFNLVNGDGPGVGSALAAHPEVDM
                     VSFTGSTRAGIQVAKNAADTVKRVCQELGGKSANILLDDADFQKAVTRDVLGCFSNSG
                     QSCNAPTRMLVPAARLDEVKSIARAAAAMVKEGDPQAESTTMGPVVSKAQYDRIQRLI
                     QAGIDEGAELVCGGTGKPEGLETGYYVKPTIFANVRNDMTIAREEVFGPVLAILPYRD
                     EAEAIRIANDTVYGLSGYVTSASIERARNVAAQLRTGMVHLNGASTDIAAPFGGYKQS
                     GNGREWGEHGFHDFLETKAVMGFNPG"
BASE COUNT         2082 a         4331 c         4475 g         1936 t
ORIGIN      
        1 cggggccaac ttgccgccgt aggtgaactc ggccagcacg atcttgccgc gctcgacggt
       61 gagggggcag gagccgtagc cgtcatagcg tgcgtcgccg cgggcctcgc cgcgcgtcac
      121 cagcaggttg tgcgcgacca ccggcgcctg cttgcgcgct gccgcggccg tcttggcgtt
      181 cgtggtgttg cccgcgtcgc cgagcgcgaa gacgttgtcg aagccgcgat ggcgcagcgt
      241 cgccgggtcc acgtcgaccc agcccgcggc gtcggccagc gggctgacgc ggatgaagtc
      301 gggcgccttc tgtggcggca cgacgtggat catgtcgaag tcgaggttca cggtctgctg
      361 gctgccgtcg gcgttggtct ggcggaatgt cgccttgcgc gcagggccgt ctacggcgac
      421 caggttgcgg ttgaactcca gcccgatgcc ataggccccg acgtattcca tcaacgccgg
      481 cacgtagtcg ggcacgccga acaataccgc gcccgcgttg cagaaccgga tgtcgagctg
      541 cgacagcacg ccggcgcggc gccagtgatc ggccgagagg tacatggcct tctgcggcgc
      601 gccggcgcac ttgatcggca tcggcggctg cgtgaagacc gccctgccgc cgcgaagatt
      661 ctgcaccagc tgccaggtat agggggcgag gtcgtagcgg tagttggagg tcacgccgtt
      721 gcgcccgagc gtctcgggca gcccctcgat gccctgccag tcgagcttga tgcccggaca
      781 caccacgagc tgccggtacg agaccacgcg gcagccgtcg aggatcacgg cgttgcgctc
      841 gggttcgaag gcggccacgg cggacttgat ccagtgcacg ccgcgcggca gcaccgcggc
      901 catcgtgcgc gccgtgaacg cgggcttgaa cacgcctgcg cctaccagcg tccagccggg
      961 ctggtagtag tgcacgtcgg cgggatcgat gatcgcgatg tcgagcccgg gtcggcgcgc
     1021 cagcaggctc gaggccaccg cgacacctgc cgcgccgccg ccgatgatca cgatctcgtg
     1081 cgaagcatcg gccacctcca ccggcgtcct gccgccgttc atgatgcggc gcaccagacc
     1141 gcggaggtcg tagccggcct tcgcgcccgt ctcgatgatc tcgggcagcg ggatcttccc
     1201 ggattgcgac agcgcccaca tcgtggccga gcgcatgccg ctgcggcagt aggccagcac
     1261 gggcctgggc agttcctcca tcatccgccc gaactcggcg ccctgctcgt cgctgacctt
     1321 gcccgattcc gcgggcaggt agcgcacctc gagccccgcg ctgcgcgcgg cgcgctcgat
     1381 ttcgctgaag ccgggctggt cggcggcctc cccgtcggtg cggttgcaga tgatcgagcg
     1441 gaatcccgcg ccggcaattt ccgggagatc ggcggctgcg atctgccccg cgaccgagag
     1501 cgcgggggtc agtttcttga tgtccacggt ttcagctcct ggcgaatctg cgcaatcaag
     1561 gcattacaaa catacatgac aatccatggt tttcctccag atcgttgcgc gcaatggcca
     1621 gcgggtggtc ggtggcatgc ggtgggacaa tcgtccgcag gaatgcgggc ccacgccggg
     1681 gaggacaatc gtccgcatga atgcggaccc gcgggggcgg gttgaccgat atagtaccca
     1741 tcggtatttt aagtcacccg ggtatgccgc cgaacgatcg attgcaccgc tacacaccgg
     1801 ccgagagcgc accacatgat cagaaatctt ccggcgaaat gggatctcga ggcggacgtc
     1861 gtggccgtgg gatcgggggg cggcggcctg gcggctgcca tcaccgccca tgaccacggc
     1921 gccagcgcgc tggtcctgga gcgctgcgac caggtcggtg gcgtcaccgc ctactcgatg
     1981 ggcgaggtgt ggattccggg caatcatctc gaagccgcgc tgggcatcga ggattcgccc
     2041 gagagcggct tcgcctacat caagcgcctg tccatgggct acggtgacga cctcgcgatc
     2101 ctcaaccaga tggttcacgg gccggttgcg ctcaggtttt tcgaggagcg gatcgggctg
     2161 aagatgtgcg tggtccgcga tctgcccgat tactactacc cgcacgtcga ggcgagcctt
     2221 gccgaaggcc gctgcctcga ggcgctgccg tttcccgcgc aaacgctcgg cgactggcaa
     2281 tccaggacgc gcctgtcgcc gcacgtgccc tacggcatga cgcacgagga catattccat
     2341 aacggcggca tggccaacat gatgaagtgg gactacagcg tcatggcgga gcgcctcgcg
     2401 aaggacgagc gctgcgccgg tcccgggctg gcggcctggt tcgtcaaggg tgtgctcgat
     2461 cgcggcatcg cgatgcacac cggcgtcgat gtgcaggaac tggtggtcga tggcgggcgt
     2521 gtcgtcggcc tgcgcgcgcg cgtcgatggc cgggacctct tcgtcaaggc caatcgcggt
     2581 gtcgtcatcg ccgtcagcag ctacgagcgc aatccgggct acgcaaagac actggggcat
     2641 cagctcgacc cggtgtcgat ggtgatgccg accatcgacg gggcgcatct gcgcctggca
     2701 gggcaggccg gggcgcgcat cgcgcgggtg ccggacgtgt cgatgctcgg tttccacgtg
     2761 ccgggcgagg agcaggagga cgaggtggcg ctgtggcgcg gcgcgctgcc cttcatgggg
     2821 ctgccgcaca cgatcgtcgt caaccgcgcc ggcaagcgct tcgccaacga ggcgttctac
     2881 cgctcggttt atttcgcgct cggcgccatc gacggcgcga cccagaccca tcccaactac
     2941 ccctgctggg cgatcttcga cagccaggcg cgcgagaagt atcccttcgg ctcggtgatg
     3001 cccggcgagg aactgcccga ggccatgggc gtcacggcgc attcgattgc cgaactggca
     3061 gcgaggatcg gcgtggacgc gcacgggctc gccgacaccg ttgcccgttt caacgattac
     3121 tccgcgcgcg gggaggatcc cgaattcgcg cgaggcacct tcccgtgggg tgcgctgatg
     3181 tgcggcgatc cgctgcagaa acccaacgcc aacctcggtc cgctgctgaa ggccccgttc
     3241 catgcggtcc ggctgcacca gatggccggc ggcggcatcg ccgccacggg catcctggcc
     3301 gaccatcact gccgcgccat cggctgggat gacgagccga tcgaggggct ctacgtcgcc
     3361 ggcaactcgg tcgcgcggct cgacaacggc gccctgatgc agagcggcat caccaacgcg
     3421 cgcggcatga cgcacggcta tctggccgga cggcacgccg ccggccaccc cagcgcgctg
     3481 ttgcagcaag agcgcttgcg cctgggcctc tgagaaagag catgatcgaa gcacagaact
     3541 ccatcgtcat cggcgccccc atcggcaacg tctgggacta cgtgcaggac atcagccgct
     3601 gggcaaacct ctttccgggt tgccgcgaat gcaccgtcat cgacgaccac aactcgcgct
     3661 ggctgatcaa ggtcggcgcc ggcgggctgg tgcgcaccgt caacgtgatg gtgcgggtcg
     3721 agcgctggga cgggccggaa cgggtcgact tctcctacgc gctggaggcc gagccggtcg
     3781 tcggcggcgg cagctacgtc gcggcgatga aggagccgca cgagacagaa atcacgctga
     3841 aggtgcgtgt cgagggcagc ggctcgatgg cgccgatgtg ggaggccgtc agcaggccgc
     3901 tgttgccgca attggcgaag agtttctccg gcaggctgaa gacggaaatc gagaacgggg
     3961 cgactgctgc cctcgcgacc gcctccgcga cgccggcaag gcccgcgccg ctcaggctca
     4021 tcgctacatg gctgcgcagg acatggcaaa agctgttcgg atagacgcca ccgtgcccag
     4081 ccggcgggct gccccggtca cacggatccg gtccaaccgc gcgcgcgggc gtcccagccc
     4141 cgaggacgcc gccgccatcg accacgagct gctggatatc gcgctcgggt tgttcctgca
     4201 acacggctac ggcggcacct cgatgcgcca gatcgtggct gccgcgggcg tctccaagac
     4261 gacgctgtac tcccgcttca cctccaaggc cgatctgttc cgggccatca tgatccagca
     4321 gatcgaccgg ctgtcgccat cggcctcgct gcaatccgaa ggcggaccgc tcgcgctcga
     4381 agcgggtttg cgctcctacg ccaaccagat gctcgccgtc agcctggagg gcgatgttct
     4441 cggcgtgaac cgcctgatct acagcgaatc ccatcgcttc ccggaactgg gcgccgccgc
     4501 ggcggagcgc accgcgctgg gtatcgcgcg catatccgac ttcgtcagga cctgcgcgcg
     4561 caacgccgga cgcagctgca gcgatcccaa gggcatcgcg gaagcgttca tcttcatgat
     4621 tcgtggctgg tacgtgaatg tccttctcac caatcgcgag gtcccggtcg ccgaacgcga
     4681 acgctgggtg aagcgcgccg tgcgcgcgct ggtgtgcgga cagcacgaat ggtgaatccg
     4741 cgacggggcg tggccgggcg cgcggcagcg ccagggcctg cgcggttcag cgcgccgcgc
     4801 gcagcgcccc ggcaagccct tcgagcagcg ccagcgtgtc gtcgagcgcg aggcaggcgt
     4861 cggtgacgct ctggccgtaa cgcagcgtgt cgcgcgggcc gagtttctgc gcgccgccct
     4921 gcaggtggct ctcgatcatc acgccgcgaa tgccgcgctg ccccgcgcgg atctgtgacg
     4981 caagatcagc cgccacatcc gcctggcgcg ccggatcctt gccgctgttg ccatggctgc
     5041 aatcgaccac caggcccgcc tcgaggccgg catcctgcag gcgttgcagc gcatcgttca
     5101 cggcggcggc gccgaaattc ggccccgaga cgttaccgcc gcgcaggacc aggcagcaat
     5161 ccggattgcc gctcgagcgc agtatcgcgg ggctgccttc cttggtgatc gaggggaacc
     5221 agtgcggatg cgcggcggcg cggatcgcgt cgatggcgac accgacgtcg ccgtcggtgc
     5281 ggttcttgat gcccaccggc atcgacaggc ccgaggcgag ctcgcggtgg acctggctct
     5341 cgacggtgcg cgcgccgatc gcgccgaagc tgaccaggtc cgcgtagtac tggccgaacg
     5401 tggtgtcgag gaactccgtg gccgcgggca ggccgagcgc ggtcacgtcg agcagcagcc
     5461 ggcgcgcgag atgcaagccc ttgttgatgt ggaagctgcc gtcgaggtcc ggatcgttga
     5521 tcaggccctt ccagccgacc acggtgcgcg gcttctcgaa atacacgcgc agcacgatga
     5581 acagcgcgtc cccgaggccc gcggtgcgca actgcatctg ccgggcgtaa tcgagagccg
     5641 ccgccggatc gtgcacggaa cagggcccga cgatgcacag caggcggcgg tccgcgccac
     5701 gcaggatcgc ctcgatcgtg cgccgcgagc tgttgaccag caccgcgccg gcgtcgctgt
     5761 gcggcagttc ctcgcacagc acggccggcg tgatcagcgg ctgggtctgg gttatgcgca
     5821 ggtcgtcggt gcggatcatg gctcggtact ccccggcgcc gcggccgctt gcggcgcgat
     5881 ccaggcgcgc aacagctcgt tgccgagcac cagcaccacc gcgccgacga acagtccgat
     5941 gatgccctgg gtgatgaagc ccccgatggc gccgacgaat atcacggcga cgggcgcgtt
     6001 caggccgcgt cccatcagca gcggcttcag cacgttgtcc agcggcatca gcagcagcgt
     6061 ccaggcgagg aaggccgccg cggtgaagag gtcggctgtc gagaacagcc agatcgcagc
     6121 cggcaccatg atgatgccca caccgagctg caccacgccg agcaccagcg ccagcgccgt
     6181 ccagaatccg gccgcgggaa cgccggccag gaacagggcg gcgccggcca gcagggcctg
     6241 gatcagcgcc acgccgagga tgccgcgcgt gacaccgcgc acggtggcgg ccgcgagcgt
     6301 cgtgaactcg gcaccgcgct ccccgacgag ccgcgtcgcc agtgcgcgtg ccacgttgcc
     6361 cccggtcgtc gcactggcca gcagcgcgcc gctgatcgcg atggcgaaca cgaactgcag
     6421 caggcccaga ccgaactgcg ccgccgatga caggaaccag cccgccaccg gtttcatctg
     6481 cggacccagg gtggcgaaca cggattccag gttctgcgac gcctgcagcc agagcgcatg
     6541 cacgcgttcc ccgacgatgg gccacgcagc gaccttgtcc gacggcggcg gcaccttgag
     6601 cttgccttcc tggaaggccc ccatcaccgc ctggacgtcg tcgaccacgg tacccgtcag
     6661 catcacgccg gggacgatga tcagcgcgag cagggccagc accagcagcg tggcggcgag
     6721 gacgcggcgc ccgccgagcc actggctgag caccaggaac ggaccgtgga gcgcgatggc
     6781 gatgatgcag ccccaggcga tcgccagcag gaagggtgcg acgatcaggg cgcaccagcc
     6841 gatcagcagc gccagtgcgc cgagccggat cgccacgtcg gtggcgcgcg cgagctcgcg
     6901 ctgctgttcg tttccgtccg tctccatggc gggtctcccg atcggtgtgc gccggcgctc
     6961 agcgagcgcg cctgctgtgt ttctcgaaat tctcctgccg tgcccgctcg ctcttgcgca
     7021 gcaggatcac ggtcgcgccg gtgccgccga ggtggcgcgg cgcggaatgg aaggccagca
     7081 cctcggcgaa ctgcggcagc cacatcgcca cgcagctctt cagcagagcc ggcgtgctgc
     7141 gcccggcgcc cttgccgtgg ttgatcagcg cggcgcgcac gtcgtgcttc atgcagtccg
     7201 cgacgaagcg ccacagggcc tcgcgcgact gctccaccgt gaggccgtgc aggtcgaggc
     7261 gcgaatcgaa gcggtagtcg cccaggcgca ggcgcttgaa cacgccgtat tgcacgccgg
     7321 ggcggatgaa gctcagttcc gcatgcggtt cgaccagcgg aatgtgggaa tcggagagga
     7381 agttgccgtc cttgcgcacc agggattcgg ccgcgagccg ccgggccagc gtgccgggcg
     7441 tgatctcggg cgcgcgcttg agatgcagtt gcacatcgcc ggcgagcggt cgcacatcgc
     7501 tcatcagctc gcgaaaatcc tcgtcctcgg acgtgttttt ctgcatcccc ggttcccctg
     7561 tccggcgccc gcgcgggccg tgccggcggc acctgccgtg gcggcgcagt atacgcgcaa
     7621 tccgcgccgc ggatgccggg catgagccat cgaaatggct gaattgccgg gtgcttgccg
     7681 ctacactgac agcccgctgc cgtgcgccac ctgcgacaat gcgaggcctc gatgacaaca
     7741 agaatggagg aaacccgatg ctgggtctga tcatgaacag gcagctgacg atcacctcga
     7801 tcctggagca cgcgcgcaag ttccatggcg acgccgaagt cgtgtcggtc acggccgacc
     7861 agccgctgca ccgttacacc tatcgcgagg ccttcgcgcg cagcgcgcgg ctggccaatg
     7921 cgctggcgcg cctcggtatt cgcccgggcg agcgcatcgg cacgctggcc tggaacgact
     7981 accgccacct cgaggtctac ttcgcgactt cctgcgcggg cttcgtgtgc cacacgatca
     8041 acccgcgcct gttcccggag cagatcgaat acatcgtcga gcacgccgag gaccgctgga
     8101 tcttcgtcga cccggccttc gtgccgctgc tggaagcgct gaagcaccgg ctcggctgcg
     8161 tcgagggttt cgtgatcctc gggccggcgg gcgcggcggc gaacaccacg ctgcgcaatg
     8221 ccgtcgacta cgagacgctg atcgccggcg agcccgatac cttcgactgg ccggcactcg
     8281 acgagaacag cgcctgcgcg atgtgctaca cctccggcac cacgggaaat cccaagggag
     8341 tggtctacag ccatcgcgcg ctcgtactgc acacctacgc ctgcctgatg cccgacgtga
     8401 tgagcctctc ggcgcgcgag gtcgtgatgc cggtggtgcc gatgttccac gtcaacgcct
     8461 ggagcatccc ctacggcgcc gccgcggtcg gcgcgaagct ggtgttcccg ggtccgaaga
     8521 tgggcgacgg cgagacgctg cagcggctga tcgagggcga gggcgtcacg gtgacggccg
     8581 gcgtgcccac ggtctggctc gcgctgctga cgcacctgga ccggaccggc aagcaggttc
     8641 cctcgctgag gcgggtgatc gtcggcggct cggcctgccc gcgtacgatc atggaagagt
     8701 tcgagcacaa gcatggcgtg cacgtgcacc atgcctgggg catgaccgag atgagcccga
     8761 tggggacctt caattctccg agcgcggatt tcgacacgct gagcagcgag gagcaatggg
     8821 cgcagcgcct gcgcgtcggg cggcccgtct acggggtgga ggtcaagatc gtcgacgccg
     8881 cgggcaagga actgccctgg gacggtgtat ccttcggcgc gctcaaggtg cgcgggccgt
     8941 ggatctgtgc ggagtacttc cgcatcggca agggcgatgc gcacgacgac gagggctggt
     9001 tcgagaccgg cgatgtcgcg gccatcgacc ggcgcggcta cgtcgcgatc accgaccgca
     9061 ccaaggacgt gatcaagtcc ggcggcgagt ggatcagctc gatcgatctc gagaacaccg
     9121 cggtggcgca cccggccgtg gccgaggcgg cggtgatcgg cgcgtatcat ccgcgctggg
     9181 acgagcggcc gctgctctgc gtcgtgttgc atccgggtga acagttgacg cgcgaggata
     9241 tgctcgcgtg gttcgagggc aaggtcgcga agtggtgggt tcccgacgac gtggtcttcg
     9301 tcgacggact cccgcacact gcaacgggca agttgagcaa gaaggatctg cgcgagcgca
     9361 tgaaggatta ccgcttcccg taggtcgcca ttcatggcga cgggtgccct gccggcggct
     9421 gcagtcgcca tgaatggcga cccacgggtc gacggcagca cccgggcatg cacacagatg
     9481 aatctggtct tgtaacggag agacgatatg agcgattaca aagcccctgt cacggagatc
     9541 gccttcgtgc tggatgagca cgccggcatg gcaggaatcg ccgcgctgcc ggggctggag
     9601 gaagcgaccc ccgacgtcgt gcaggcggtg ctggagggcg tggccacgct gaccgaggag
     9661 gtgatcgcgc cgctcaactg gccgggcgac cgccagggca cgcgcatcga gaaccgcgcc
     9721 gtggtgcagg ccgacggttt caaggaggcc tactggcagt acgtcgaggg cggctggggc
     9781 agcctgccgt ttccggccga gctcggcggg cagggcatgc ccgtggtgct cgccaccgcg
     9841 gtgctcgaga tggtccagtc gggcggcatt gccttcggcc tgtgcccgct gctcacccag
     9901 ggcgccaccg atgcgatcct cgcccacggc agcgatgaac tgaagcgcac gtacctgccg
     9961 aagatgatct cgggcgaatg gaccggcacc atgaacctca ccgaaccgca ggcgggctcc
    10021 gatctcgccg cggttgcggc acgcgcgcaa cgcgacgggg atcattaccg catcttcggc
    10081 aacaagatct tcatcacctg gggtgaccac gggatgacgg agaacgtgat ccacctggtg
    10141 ctcgcacgcc tgcccgacgc gccgccgggc gtgagaggca tctcgctgtt cctggtcccg
    10201 aagttccttg tgaatgccga cggcagcccg ggcgcgcgca acgacgtcta cccggtctcg
    10261 gtcgagcaca agctcggcat tcacgccagc ccgacctgcg tgatgggctt cggcgacgac
    10321 ggcggcgcca tcggctacct ggtcggcgag gagggcaagg gcctcgcctg catgttcacg
    10381 atgatgaacc acgcgcggct ctcggtgggg ctgcaggggg tggcggtctc cgagcgatcg
    10441 ctgcagcgcg ccgtggcctt cgcgcgcgag cgggtacagg gcgcggtggc cggacagccc
    10501 gggcgtgccc cgatcgtcca tcaccccgac gtgcggcgca tgctgctgca gatgaaggcg
    10561 ctgaccgagg ccgggcgcgc gctcgcctac gtcgcgatgg gcagccagga ccgcatccat
    10621 cattcgcccg atgccgccga gcgagcgttc cacgaggaac gcatcgcgct gctcacaccg
    10681 atcgtgaagg gctggtgcac ggaaatgtcg atggagatca cctcgctcgg cgtgcaggtg
    10741 catggcggca tgggtttcat cgaggagacg ggcgccgcgc agcacctgcg cgatgcgcgc
    10801 atcctgccga tctacgaagg caccaacggc atccaggcgc tggatctggt ggggcgcaag
    10861 ttcctgcgtg acggcggtgc cgggatgaag tcgctgctcg ccgagatgcg cggcgtcgcg
    10921 acggcgctcg gcgcgggcgc gctcgccgac atcgggacgc ggctcgcgcg cgcgatcgat
    10981 gcgctgcagg atgcggccga ctggatccgc gcgaatgccg atgccgccgg cgtggtgccg
    11041 ggcgcggcgg catacaactt cctgatgctc gcgggcaccg tggccggtgg ctggcaatcg
    11101 ggcgtggcgg cgctggccgc gcagcgccgg ctcgcggcgg gtgccgacaa cgcggacttc
    11161 ctgcgcgcca agctgctcac ggcgcgcttc tacgccgagc agatcctgcc gcgcgcgcag
    11221 atgcatgccg aggccgtgca ggccggtgcg cagacgatca tggccttcga cgaggcgatg
    11281 ttgtaggtcg ccattcatgg cgacatgtgc attcggccag gcacccgtcg gcatgaatgc
    11341 cgacccacac cagaaggagt gatgacgatg caacgctgcg aacagttcta catcaacggt
    11401 cagtgggtcg atccggtcga gcgccgcacg ctggaggtga taaacccggc caccgagcag
    11461 ccgatcgcga caattgcgct gggctcggcg gcggacgtcg acaaggcctg cacggccgcg
    11521 cgtgctgcat tcccggcgta ttcgcgcacc tcgcgcgccg agcgcatcgc gctgctcgag
    11581 cgcatcatcg cgggctacca ggcgcgctat ggcgaattcg ccgcggtcat ctcgcaggag
    11641 atgggagcgc cgatcacgct cgcgaccagg gcccaggcgg ccatgatcgt cgggcatttc
    11701 agcgccgcgc tgcaggtgct gaaggaattc cgcttcgagg aggacatcgg gaccacgcgc
    11761 gtgttgcgcg agcccatcgg cgtgtgcgcg ttcatcacgc cgtggaactg gcccgcgaac
    11821 cagatcgcct gcaaggtggc cccggcactc gccaccggct gcaccatggt gctgaagccg
    11881 agcgagatcg cgccgctcga cgcgtacctg ttcgcgcagg tgctgcacga tgccggcgtg
    11941 ccgccgggcg tgttcaacct cgtcaacggc gacggtcccg gcgtgggcag cgcgctggcc
    12001 gcgcacccgg aagtcgacat ggtctcgttc accggctcca cgcgcgcggg catccaggtg
    12061 gcgaagaacg ccgccgacac ggtcaagcgc gtgtgccagg aactcggcgg caagtcggcc
    12121 aacatcctgc tcgacgatgc ggacttccag aaggccgtga cgcgcgacgt gctcggctgc
    12181 ttcagcaaca gcggccagtc ctgcaacgcg cccacgcgca tgctggtgcc tgcagcgcgt
    12241 ctcgacgagg tcaagtcgat cgcgcgcgcc gccgccgcga tggtcaagga aggcgacccg
    12301 caggcggaaa gcaccacgat gggcccggtg gtcagcaagg cgcaatacga caggatccag
    12361 cgcctgatac aggccggcat cgacgagggc gcggaactgg tctgcggcgg caccggcaag
    12421 cccgaggggc tggagaccgg ctactacgtg aagcccacga tttttgccaa cgtgcgcaac
    12481 gacatgacca tcgcgcgcga ggaagtcttc gggccggtgc tcgcgatcct gccctaccgc
    12541 gacgaggccg aggcgatccg catcgccaac gacaccgtct acgggctgtc gggctacgtc
    12601 acctcggcga gcatcgagcg cgcgcgcaac gtggcggcgc aattgcgtac cggcatggtg
    12661 cacctgaacg gcgcctcgac cgacatcgcc gcgcccttcg gcggctacaa gcagtcgggc
    12721 aacggccgcg agtggggcga gcacggcttc cacgacttcc tcgagaccaa ggcggtgatg
    12781 ggattcaacc cgggctgatg ccggggcccc tgcgcgcatc ggcc
//
LOCUS       DAORUQ010000095        12763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4896_length_12763_cov_6.155020, whole genome shotgun sequence.
ACCESSION   DAORUQ010000095 DAORUQ010000000
VERSION     DAORUQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12763
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4896_length_12763_cov_6.155020"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1486
                     /locus_tag="PLW13_09550"
     CDS             116..1486
                     /locus_tag="PLW13_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HQY70662.1"
                     /translation="MDPILTLAAGAVLQRPPPAHDLAIWHGVSPALWMSVIALLGGAL
                     LYSGRRPLFALQQRLEGSLDARAVFERVLAGLRAAARRITALLENGSLQRQAALFIAA
                     ALVLGGTGMLSSAAPLSGPRALLPVDGVSVAAALALAAAALGATLLHRQRLHALILTG
                     AVGLIVALVFVRFSAPDLALTQLSVEVVTVVLLLLALYFLPQNTAPESGRARRWRDGV
                     IALAAGCGTALLAWAVLTREANPISAFYLENSVPGGGGSNVVNVILVDFRGFDTFGEI
                     TVLALAALGIGALLDTLHLPGPASDAQGRAWDQDMHPLILATFARLLLPLALLVSVFI
                     FLRGHNQPGGGFIAGLVTAIALIMQYLANGASWAQARLPARTEPLIALGLGAAFLTGA
                     ASWGFGRPFLTSTFGHAQLPLVGGFELASAMAFDLGVYLVVVGATLLILINLGLAHGA
                     SHEGER"
     gene            1486..1812
                     /locus_tag="PLW13_09555"
     CDS             1486..1812
                     /locus_tag="PLW13_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HQY70663.1"
                     /translation="MEALIAMAIGVLTGCGVYLVLRARTFALVLGLTLLSYAVNLFLF
                     ATGRLVTGRPPVIGAAASHADPLPQALVLTAIVIGFAMTAFLIVLSLRARADLGNDHV
                     DGREDA"
     gene            1809..3332
                     /locus_tag="PLW13_09560"
     CDS             1809..3332
                     /locus_tag="PLW13_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HQY70664.1"
                     /translation="MSAHLAIAPVLAPFVAGLALLALAGMPLGLKRAVSVLALLAQAG
                     IAMALLAAVAQGDILVYRLGDWPAPWGIVLVADRLAAAMVLITTLLALFAAIHAGDGA
                     DRHGRHFHVLFQLQLFGLAGAFLTGDLFNLFVFFEVLLIASYGLLLHGGGAERTRAGL
                     HYVVLNLLGSTVFLFAAGLLYATLGTLNLADLAQKAAALPAQDAGLARAGGLLLLAVF
                     ALKAALLPLHLWLPAAYAGTSAPVAALFAIMTKVGAYAILRTATLLFGDTDTALANLF
                     EPWLLPLALATLGIGMLGALAAESLGRLAAYLVVASVGTLLTAFTLGAAGIAAGLYYL
                     SHGTFASAALFLLADAIRRRRPANADRLRPGPDLPRHALWGALFFVAAIAAAGLPPLS
                     GFLGKFLLLRAAPGQPLVWLVILLGALAGVIALAGAGSRLFLSSSTADGAPLQQPLPL
                     GAAGAPTPRELLAVSGLLGLIAALTLGAGPAVEFAQATAAQLLAPAAYIHAVLGVTP"
     gene            3329..3826
                     /locus_tag="PLW13_09565"
     CDS             3329..3826
                     /locus_tag="PLW13_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HQY70665.1"
                     /translation="MNGFARWLPHPLLTPVLMATWLLLNNSLAPGHLVLGLLLGLVIP
                     RFTLAFWPERVRIRRPRALLGLAGVFLQDVLLANFAVARLVLAGPRALRPAFVVVPLA
                     LRDELAISLLANIICLTPGTVSARLSADRRELLVHALDCADTVALVATIKSRFEAPLR
                     EIFEC"
     gene            3820..4089
                     /locus_tag="PLW13_09570"
     CDS             3820..4089
                     /locus_tag="PLW13_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HQY70666.1"
                     /translation="MLNVALAIAFALVGAAFVLSVVRLLRGPHAPDRILALDTLYINA
                     IALLVLFGIRLASPLYFEAALLIALMGFVGTVALAKFLLRGDIIE"
     gene            4086..4451
                     /locus_tag="PLW13_09575"
     CDS             4086..4451
                     /locus_tag="PLW13_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HQY70667.1"
                     /translation="MNPALEILLSVLVVVGALFTFIGSLGLVRLQDFYTRLHGPTKAT
                     TLGVGCLLVASSVYFSVAGEGLSLHELLVSLFLFITAPISAHLLARAALHLDLPGAVA
                     RPPPDASASPESARDTPRH"
     gene            4605..6215
                     /locus_tag="PLW13_09580"
     CDS             4605..6215
                     /locus_tag="PLW13_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY70668.1"
                     /translation="MNDYASTRRDYRWDCPARFNFARDVIDRWAAADPAQPAMLWVDD
                     HGREERRTFADIATASCRAANVLAAAGVVPGDRVVLLLGRQIAWWEVLTACLRMGAVA
                     SPGTAQLSAKDLAFRINAAQATCVVTDTGNAAKVDTVLGACPSLKARVLVDGSRDGWI
                     DYAAALRAASEAFVTVDSAAEDEALCYFTSGTTGQPKMCMHAHSYGLAHQTTGRYWLD
                     LKPSDLHWNLSDTGWAKAAWSSYFGPWNCGAAIFVHHTQGFSPRRALELLATQPITTF
                     CGAPTIYRMFVQEDLARYRFPRLRHCVGAGEPLNPEVIETWRKATGLVIRDGYGQTET
                     VLLCGNYPSLEPRLGSMGKPAPGIGLAVIDMQGRELAPGAEGDVAVRVKPRRPPGLFL
                     EYKGDPRKTAASYRGDWYLTGDRATVDADGYFWFVGRSDDVILSAGYRIGPFEVESAL
                     IEHPAVAESAVVSSPDAQRGEIVKAFVVLAPGHEASEALATELQAHVKRVTAPYKYPR
                     RIEFVEELPKTVSGKIRRVELRNREWGR"
     gene            6220..7911
                     /locus_tag="PLW13_09585"
     CDS             6220..7911
                     /locus_tag="PLW13_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY70669.1"
                     /translation="MTLALSHCSGPDDRPLLEQTIGEAFVATAGRFPDRPALVVPHQG
                     IRWNYTEYRREVDRLAAALLVLGIEPGDRLGIWAPNCVEWCLTQYASARIGAIMVCIN
                     PAYRLHELEYALNKVQCKALITAERFRSSDYLGMLQTLAPELARATPGQLDAHRLPHL
                     KAVIRMGEEHTPGMFNFDSIRAAVEAPDIARVEAVAAQLRPADPINIQFTSGTTGNPK
                     GATLTHRNILNNGSIVGEGMRLTEHDRLCVPVPLYHCFGMVMGSLACVTHGAAAIFPA
                     EAFDALATLQAVAAERCTALHGVPTMFIAQLDHPRFAQFDLSTLRTGIMAGAPCPVEV
                     MNRVLTQMHLPEILIAYGQTETSPVNHMTRADDPIGKRVGSVGRAAAHCEIRIVDAEG
                     RTLPVGERGEICCRGYAVMHGYWDDEERTRETIDAQGWLHSGDIGVMDAEGYVQVTGR
                     IKDMIIRGGENIYPREVEEFLYTHPDIQDVQVFGVPDAKYGEQVCAWIRLRPGAALDA
                     DRVRDFCRERIAHFKVPRFIRFVDDFPMTVTGKVQKFRMREQMIAELGPADPRPS"
     gene            complement(7908..8318)
                     /locus_tag="PLW13_09590"
     CDS             complement(7908..8318)
                     /locus_tag="PLW13_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70670.1"
                     /translation="MDAAAANKAIVMRYFDIIGGRLAAPLEECFTPDVRWHVPPSNPM
                     IKPNPACGIDGVMQVLSSGVGVYAPGSLDVHLEAVIADRHDVAAQFTLNALLANGAAY
                     ENRYFFRFRMADGRIAEVWEYLDTLRQHELGALG"
     gene            complement(8350..9909)
                     /locus_tag="PLW13_09595"
     CDS             complement(8350..9909)
                     /locus_tag="PLW13_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY70671.1"
                     /translation="MHQLSALDRLYLTQESPRCAAHLSALLFYSQQTAPGGRVRLRDI
                     MNVFAERAREVPFLHCRVQEVPFGLDNPYWVHDGHFNIESHVNHVALPKPGDWRQLCI
                     MAARIHARPVDRSRPLWEATVIEGLDNIEFLPRGSFAILLKVHYAAIDGLAALQALER
                     LHDKAPHAAHEAAGEAQADDGELPVREMLARAGLHALGAPLRWGRLAGNLVPALGRMV
                     AAVREQHGARSQAAVNTRFNGRISSYRVVESTFFSLAELKVVRAAVPQSTLNDVVVTV
                     IGGALRRYLQSRGELGDVSPVTIAPLSVREAEARELGGNRVSAMAIPLHTTIEDPLQR
                     LAAVQRDAAGARRIAEALGPHNALDLIEAVPSQLASLGFLEFGTRLLLATGIAVPVNT
                     VITSVPGPQTPLYLAGARLVTMAGFGPVMDSMGLFHAVMSYDGKVSIAINSCREMMPD
                     PAFYAECIVASFEELREAAKAVRPGRSATRKSAPAGARKAAGRAREPARAATARAGRK
                     PAVRRAARRRD"
     gene            complement(10007..10585)
                     /gene="amrA"
                     /locus_tag="PLW13_09600"
     CDS             complement(10007..10585)
                     /gene="amrA"
                     /locus_tag="PLW13_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQY70672.1"
                     /translation="MAPGSSAECAFGPAERATLLALAGQAIRAGLDGERLRVEVEAFA
                     PALAQPRASFVTLNLAGRLRGCMGTLEARRALVLEVAEMAHAAAFGDPRFPALAREEF
                     ERIELHISVLSPPELLVFDSEEELLGQLRPGIDGLILSERHARGTFLPSVWEQLPEPR
                     EFLARLKQKAGLDCAHWSDAVRVERYTTESIP"
     gene            complement(10563..11348)
                     /gene="amrB"
                     /locus_tag="PLW13_09605"
     CDS             complement(10563..11348)
                     /gene="amrB"
                     /locus_tag="PLW13_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQY70673.1"
                     /translation="MRAVRKPAVAGVFYPADPESLHEEVRAFLSVVPQGGEIPKAIIV
                     PHAGYVYSAPVAAAAYARLAPARGRVKRVVLLGPSHRVAFHGLSVPSCESYETPFGRI
                     ELDHAAIAALSALAQVVCLDAAHAHEHSLEVHLPFLQEVLGDFSLVPLVVGEASAEEV
                     AEVLERVWDGPGTLVVVSTDLSHYYDYETAQRLDRTTCAAIEALRFEDLGRDSACGRV
                     PLGGLLCLARRRGLRVTTVDLRNSGDTAGDRERVVGYGAWLVG"
     gene            11450..12550
                     /gene="amrS"
                     /locus_tag="PLW13_09610"
     CDS             11450..12550
                     /gene="amrS"
                     /locus_tag="PLW13_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQY70674.1"
                     /translation="MTGSERFEGSFPTRHWHTLSDGRVQCDVCPRFCKLHPGQRGLCF
                     VRGNQDDAIVLTTWGRSSGFCVDPVEKKPLNHFLPGTPILSFGTAGCNLACKFCQNWD
                     ISKSREWDTLADAATPETIARAARELGCRSVAYTYNDPAIFPEYAIDVAAACRELDIR
                     SVAVTAGYMTPAPRAELFRHMDAANVDLKCFTEDFYHKLCGAHLASILETLLYLRHET
                     TVWLELTTLLIPGLNDSDREIEEMTQWVVENLGPDVPWHFTAFHPDWKMRDIPPTPLG
                     TLLRAHAIARRNGVRYAYTGNVHDEAHGATHCHTCDTRLIGRDGYRITDWNLGPGGTC
                     RSCGSACAGLFDADPGTWGARRQPVRLLDYHV"
BASE COUNT         1836 a         4648 c         4297 g         1982 t
ORIGIN      
        1 caacaccgac cgatcgagct agtcaggtgc ataggtgaaa tcgtccgtca gggtctcgta
       61 gcgctcagcg cgcgtgcgcc tgtccctcac cgggatgtca tagcccgccc ggacggtgga
      121 cccgatcctc acgctggcgg cgggcgcggt gctgcagcgg ccaccgcccg cgcacgacct
      181 cgcgatctgg cacggtgtca gcccggcgct gtggatgagc gtgatcgcgc tgctcggcgg
      241 cgcgctgctc tactccggac gccggccgct gttcgcgctg caacagcgcc tggaaggatc
      301 gctggacgcg cgcgccgtgt tcgagcgcgt actcgcgggc ctgcgcgccg cggcgcggcg
      361 catcaccgcg ctgctggaaa acggctcgct gcagcgccag gccgcgctgt tcatcgccgc
      421 ggcgctcgtg ctgggtggca ccggcatgct ctcctccgct gcccccctga gcggcccgcg
      481 tgcgctgttg cccgtggacg gcgtgagcgt ggcggccgca ctggcgctag cggcggccgc
      541 gctcggcgcg accttgctgc atcgccagcg tctgcacgcg ctgatcctca cgggcgccgt
      601 ggggctcatc gtcgcgctgg tcttcgtcag gttttccgcg cccgatctcg cgctcacgca
      661 actctcggtc gaggtggtca ccgtcgtgtt gctgctgctg gcgctgtatt tcctgccgca
      721 gaacacggcg cccgaatcgg ggcgcgcccg ccgctggcgc gatggcgtca tcgcgctcgc
      781 cgcaggctgc ggcacggccc tgctggcctg ggcggtgctg acgcgcgagg caaacccgat
      841 ctcggccttc tacctcgaaa acagcgtgcc gggcggtggc ggcagcaacg tggtcaacgt
      901 gatcctcgtc gatttccgcg gcttcgacac cttcggcgag atcaccgtgc tcgcgctggc
      961 ggcgctcggc atcggcgcgc tgctcgacac gctgcacctg ccggggcctg ccagcgatgc
     1021 gcagggccgc gcctgggatc aggatatgca tccgctgatc ctcgcgacct tcgcgcgcct
     1081 gttgctgccg ctggcactgc tggtttccgt gttcattttc ctgcgcggtc acaaccagcc
     1141 gggcggtggc ttcatcgcgg gcctggtcac ggccatcgcg ctgatcatgc agtatctggc
     1201 caacggcgcg agctgggcgc aggcgcgtct tcccgcccgt accgagcccc tgatcgcgct
     1261 gggcctcggc gccgccttcc tgaccggcgc ggcgagctgg ggcttcggcc gaccgttcct
     1321 gacctccacc ttcggtcacg cgcaactgcc gctggtcggg ggcttcgagc tggcctcggc
     1381 catggccttc gatctcgggg tttacctggt cgtggtcggc gcaacgctgc tgatactgat
     1441 caacctcggc ctggcgcacg gcgcgagcca cgagggagaa cggtgatgga ggccctgatc
     1501 gcgatggcca tcggcgtgct cacgggctgc ggcgtctacc tggtattgcg cgcgcgcacc
     1561 ttcgcgctgg tgctggggct gaccctgctc tcctatgccg tcaacctctt cctgttcgcc
     1621 accggccgtc tggtgactgg ccggccgccg gtgatcggcg ccgctgccag ccatgcggac
     1681 ccgctgccgc aggcgctcgt gctcaccgcg atcgtcatcg gcttcgcgat gacggccttc
     1741 ctgatcgtgc tgagcctgcg cgcgcgcgcg gacctcggca acgaccacgt cgacggacgc
     1801 gaggacgcat gagcgcgcat ctcgccatag cccccgtgct ggcaccgttc gtcgccggcc
     1861 tcgcgctgct cgcgctcgcc ggcatgcctt tggggctcaa gcgcgcggtc tcggtgctgg
     1921 ccctgctggc gcaggccggc atcgcgatgg cgcttctcgc cgccgtggcg cagggcgaca
     1981 tcctcgtcta tcgtctcggc gactggccgg ccccctgggg catcgtgctg gtggcggacc
     2041 gcctcgccgc ggcgatggtg ctgatcacca cgctgctggc gctgttcgcc gcgatccatg
     2101 ccggcgacgg cgccgaccgg cacggacggc atttccacgt actgttccag ctgcagctgt
     2161 tcgggctcgc gggcgccttt ctcaccggcg acctgttcaa cctcttcgtg ttcttcgagg
     2221 tgctcctgat cgcctcctac ggcctgctgc tgcacggcgg cggggccgaa cgcacgcgcg
     2281 ccgggctgca ctacgtggtg ctcaacctgc tcggctcgac ggtgttcctg ttcgccgccg
     2341 gactgctcta cgccacgctg ggcaccctga acctcgccga cctcgcacag aaggccgcgg
     2401 ccctgcccgc ccaggacgcg ggactggcac gcgccggcgg cctgctgctg ctggccgtct
     2461 tcgccctgaa ggcagcgctg ctgccgctgc acctgtggct acccgcggcc tatgccggca
     2521 ccagcgcgcc ggtggcggca ctgttcgcga tcatgaccaa ggtgggcgcg tacgcgatcc
     2581 tgcgcaccgc cacgctgctc ttcggcgaca cggacaccgc gctggccaac ctgttcgaac
     2641 cctggctgct gccgctcgcg ctggccacgc tgggcatcgg catgctgggc gcgctcgccg
     2701 ccgagagcct ggggcgtctt gccgcctacc tcgtggtggc ctcggtgggc acgctgctca
     2761 ccgccttcac gctgggtgcc gcgggcattg ccgccgggct ctactacctg tcgcacggca
     2821 ccttcgcgag cgccgcgctg ttcctgctgg ccgatgcgat cagaaggcgt cgcccggcga
     2881 acgcggaccg cctgaggccg ggcccggacc tgccgcgcca cgcgctctgg ggcgccctgt
     2941 tcttcgtcgc cgcgatcgcc gcggccggcc tgcccccgct gtcgggcttc ctcggcaagt
     3001 tcctgctcct gcgcgcggcg cccggccagc ccctggtgtg gctcgtgatc ctgctcggcg
     3061 cgctggccgg cgtgatcgcg ctcgcgggcg cgggtagccg gctcttcctg agctccagca
     3121 cagccgatgg cgcgccgctg cagcagccgc tgccgttggg cgccgcggga gcgcccacgc
     3181 cacgcgaact gctcgccgtg agcggcctgc tcggactgat cgccgcgctc acgctgggcg
     3241 cggggccagc ggtggaattc gcgcaagcca ccgccgcgca actgctcgcg ccggcggcct
     3301 acatccacgc ggtgctggga gtgacgccat gaacgggttc gcgcgctggc tgccgcatcc
     3361 gctgctcacc ccggtgctca tggcaacgtg gctgctgctg aacaacagcc tcgcgccggg
     3421 acacctcgtg ctcgggctgc tgctggggct ggtgatcccg cgcttcacgc tcgccttctg
     3481 gcccgagcgg gtgcgcatcc gccgcccccg cgcgctgctc ggcctcgcgg gcgtgttcct
     3541 gcaggatgtg ctgctcgcca atttcgccgt cgcgcggctg gtccttgcgg gcccgcgcgc
     3601 gctgcgcccg gccttcgtcg tggtgccgct cgcgctgcgc gacgagctcg cgatcagcct
     3661 gctcgccaac atcatctgcc tcacgccggg cacggtatcg gcacggctct cggcggaccg
     3721 acgggagctg ctggtccatg cactggattg cgccgacacc gtggcgctgg tcgccaccat
     3781 caagtcgcgc ttcgaggcgc cgctcaggga gatcttcgaa tgctgaacgt cgcgctggcc
     3841 atcgccttcg cgctggtcgg ggccgccttc gtgctcagcg tcgtgcgact gctgcgcggg
     3901 ccgcacgcgc cggatcgcat cctggcgctc gacacgctct acatcaacgc catcgccctg
     3961 ctggtgctgt tcggcatccg gctggcgagc ccgctctatt tcgaggcggc cctgctcatc
     4021 gccctgatgg gcttcgtcgg caccgtggcg ctggcgaagt tcctgctgcg cggagacatc
     4081 atcgaatgaa ccccgcgctc gaaatcctgc tgagcgtcct ggtggtcgtg ggcgcgctct
     4141 tcaccttcat cggctcgctg ggactggtcc gcctgcagga tttctacacg cgcctgcacg
     4201 gcccgaccaa ggcgacgacg ctgggggtgg gttgcctgct cgtcgcgtcg tcggtctatt
     4261 tcagcgtcgc cggcgagggc ctcagcctgc acgagttgct ggtgtcgctg ttcctgttca
     4321 tcaccgcgcc gatttccgcg cacctgctgg ccagggccgc gctgcacctg gacctgcccg
     4381 gtgccgtggc gcgtccaccg cccgacgcga gcgcatcgcc cgaatcggcg cgcgacacgc
     4441 cacggcactg aaccgcccgt cgcgcggcca gccccagcgg cattgacggc gcccggcgca
     4501 tcgtcatact gcccgagccc gggccgccgg tgttcaaacc atcagcggcg aagcgctatc
     4561 gatcgatcgc ggccgggcgc aaagcgataa cgaaacggca gacgatgaac gactacgcaa
     4621 gcacccgccg cgactaccgc tgggattgcc cggcacgttt caacttcgcg cgcgacgtga
     4681 tcgaccgctg ggcagcggcc gatcccgcgc agcccgcgat gctgtgggtc gacgatcacg
     4741 gccgcgagga aaggcgcact tttgccgata ttgccacggc ctcgtgccgc gccgccaacg
     4801 tgctcgccgc cgcgggggtg gtgccaggcg atcgcgtcgt gctgctgctg ggccggcaga
     4861 tcgcgtggtg ggaagtactg accgcctgcc tgcgcatggg cgcggtcgcg agccccggca
     4921 cggcgcagct ctcggcgaag gatctcgcct ttcgcatcaa cgcggcgcag gccacctgcg
     4981 tggtgaccga caccgggaat gccgcgaagg tcgacacggt gctcggcgca tgcccgagcc
     5041 tgaaggcgcg cgtgctggtg gacggatccc gcgacggctg gatcgactac gccgccgcgc
     5101 tgcgcgcggc atccgaggcg ttcgtcaccg tcgacagcgc ggccgaggac gaggcactgt
     5161 gctatttcac ctcgggcacg accggtcagc cgaagatgtg catgcacgcg cattcctacg
     5221 ggctggcgca ccagaccacg gggcgctact ggctggacct gaagccctcg gacctgcact
     5281 ggaacctgag cgacaccggc tgggccaagg cggcgtggag cagctatttc gggccgtgga
     5341 actgcggcgc ggcaatcttc gtgcaccaca cgcagggctt cagccccagg cgcgcgctcg
     5401 agctgctggc gacgcagccg atcaccacgt tctgcggcgc cccgaccatc taccgcatgt
     5461 tcgtgcagga ggacctcgcg cgttaccgct tcccccgcct gcgccactgc gtgggcgccg
     5521 gcgagccgct gaaccccgag gtgatcgaga cctggcgcaa ggccacggga ctggtgatcc
     5581 gcgacggcta cggccagacc gagaccgtgc tcctatgcgg caactacccg tcgctggagc
     5641 cgcgcctggg ctcgatgggc aagcccgcgc ccggcatcgg gctggcggtg atcgacatgc
     5701 aaggccgcga actggcgccg ggcgccgagg gcgacgtggc ggtgcgcgtg aagccgcggc
     5761 gcccgcccgg gctgtttctc gagtacaagg gcgatccgcg caagacggcc gcgagttacc
     5821 gcggcgactg gtacctcacc ggcgatcgcg ccacggtgga cgccgacggt tacttctggt
     5881 tcgtcggccg ctccgacgac gtgatcctct cggccggcta ccgcatcggt cccttcgagg
     5941 tcgagagcgc gctgatcgag cacccggccg tggccgaatc cgcggtcgtc agcagcccgg
     6001 acgcgcagcg cggcgaaatc gtgaaggcct tcgtggtgct ggcgcccggc cacgaggcca
     6061 gcgaggcgct ggccaccgaa ctgcaggcgc acgtgaagag ggtcaccgca ccgtacaagt
     6121 acccgcgccg catcgagttc gtcgaggagt tgccgaagac cgtgagcggc aagatccggc
     6181 gcgtggaact gcgcaaccgc gaatggggac gctgaggccg tgacgctcgc gctcagccat
     6241 tgcagcggtc ccgatgaccg cccgctgctc gagcagacca tcggcgaggc cttcgtcgcc
     6301 accgccgggc gcttccccga ccgcccggcg ctggtggtgc ctcaccaggg catccgctgg
     6361 aactacaccg aataccggcg cgaggtcgat cgcctcgcgg ccgcactgct cgtgctcggc
     6421 atcgagcccg gcgatcgcct cggcatctgg gcgcccaact gcgtcgagtg gtgcctcacg
     6481 cagtacgcga gcgcgcgcat cggcgcgatc atggtgtgca tcaaccccgc ctaccgactc
     6541 cacgagctcg agtacgcgct gaacaaggtg cagtgcaagg cgctgatcac ggccgagcgc
     6601 ttcaggagca gcgactacct cggcatgctg cagacgctgg caccggaact cgcgcgcgcc
     6661 acgccggggc agctcgatgc acaccggctg ccgcacctga aggccgtgat ccgcatgggc
     6721 gaggagcaca cgcccggcat gttcaatttc gattcgatcc gcgccgcggt ggaagcgccc
     6781 gacatcgcgc gcgtcgaagc cgtcgccgcg caactacgcc ctgccgaccc gatcaacatc
     6841 cagttcacca gcggcaccac cggcaacccc aagggcgcga cgctgacgca ccgcaacatc
     6901 ctgaacaacg gcagcatcgt cggcgagggc atgcgcctca cggagcacga caggctgtgc
     6961 gtgcccgtgc cgctctacca ctgcttcggc atggtgatgg gcagcctggc gtgcgtcacg
     7021 cacggtgccg cggcgatctt cccggcggag gccttcgatg cgctcgccac gctgcaggcc
     7081 gtggccgccg agcgctgcac cgcgctgcac ggcgtgccca cgatgttcat cgcgcagctc
     7141 gatcacccgc gcttcgcgca gttcgacctg tcgacgcttc gcaccggcat catggccggc
     7201 gccccctgcc cggtcgaggt gatgaaccgc gtgctgacgc agatgcacct cccggagatc
     7261 ctgatcgcct acggccagac cgagaccagc cccgtgaacc acatgacgcg cgccgacgac
     7321 ccgatcggca agcgcgtggg ctcggtgggc cgcgcggccg cgcattgcga gatcaggatc
     7381 gtcgatgccg agggacgcac gctgcccgtg ggcgagaggg gcgagatctg ctgtcgcggc
     7441 tacgccgtga tgcacggcta ctgggacgac gaggaaagga cgcgcgagac catcgacgcg
     7501 cagggctggc tgcactcggg cgacatcggc gtcatggacg ccgagggcta cgtgcaggtc
     7561 accggccgca tcaaggacat gatcatccgc ggcggcgaga acatctatcc gcgcgaggtc
     7621 gaggaattcc tctacaccca tcccgacatc caggacgtgc aggtattcgg cgtgcccgac
     7681 gcgaaatacg gcgaacaggt ctgcgcgtgg atccgcctgc gccccggcgc ggcgctggat
     7741 gccgatcggg tcagggactt ctgcagggag cggatcgcgc acttcaaggt gcctcggttc
     7801 atccgcttcg tcgacgactt ccccatgacc gtcaccggca aggtgcagaa attccgcatg
     7861 cgcgagcaga tgatcgcgga gctcggcccg gctgatccgc ggccttccta gcccagggcc
     7921 ccgagctcgt gctggcgcag ggtatcgagg tactcccaga cttccgcgat gcgcccgtcg
     7981 gccatgcgga aacggaagaa atagcggttc tcgtaggcgg caccgttcgc gagcaacgcg
     8041 ttcagcgtga actgcgccgc cacgtcgtgg cggtcggcga tcacggcttc gaggtgcaca
     8101 tctaggctcc ccggcgcgta gacgcccacg cccgagctca gcacctgcat cacgccgtcg
     8161 atgccgcacg cggggttggg cttgatcatc gggttgctgg gcggcacgtg ccagcgcaca
     8221 tccggcgtga agcattcctc gagcggcgcg gcgagcctgc cgccgatgat gtcgaaatag
     8281 cgcataacga tggccttgtt ggccgctgcg gcatccatga tccctcctgc ttcccgtgcc
     8341 tcgggacact cagtcgcgcc ggcgcgcggc acggcgcacg gccggctttc gcccggcccg
     8401 cgcggtcgcg gcgcgcgccg gttcacgcgc tcgtccagcc gccttgcgtg cgcctgccgg
     8461 cgcgctcttc ctcgtcgcgc ttcggcccgg ccgtactgcc ttcgccgcct cgcgcagttc
     8521 ctcgaacgag gccacgatgc actcggcgta gaacgccggg tccggcatca tctcgcggca
     8581 cgagttgatg gcgatggaca ccttgccgtc gtagctcatg acggcatgga agagccccat
     8641 ggaatccatc accggaccga atccggccat ggtgaccagg cgcgcgccgg ccaggtacaa
     8701 gggcgtctgc ggcccgggca cgctggtgat cacggtgttc accggcaccg cgataccggt
     8761 ggcgagcagc aggcgcgtgc cgaactccag gaaacccagc gaggcgagct gcgagggcac
     8821 ggcctcgatg aggtccagcg cgttgtgcgg gcccagtgcc tcggcaatcc tgcgggcgcc
     8881 ggcggcatcg cgctgcaccg ccgccaggcg ctgcagcgga tcctcgatcg tggtgtggag
     8941 ggggatcgcc atcgcgctca cccggttgcc accgagttcg cgcgcctcgg cttcgcgcac
     9001 gctgagcggc gcgatcgtca ccggcgacac gtcgccgagt tccccgcgcg actgcaggta
     9061 cctgcgcaac gcgccgccga tcaccgtgac caccacgtcg ttcagggtgc tctggggcac
     9121 cgcggcgcgc accaccttca actcggccag ggagaagaag gtactctcga ccacgcggta
     9181 cgatgaaatc cgcccgttga aacgcgtgtt gaccgcggcc tgggagcgcg cgccgtgctg
     9241 ctcgcgcacc gcggcgacca tgcgcccgag cgccggcacc aggttgccgg ccaggcgccc
     9301 ccagcgcagc ggcgccccca gcgcgtgcag tccggcgcgc gcgagcatct cgcgcaccgg
     9361 caattcgccg tcatcggcct gcgcttcgcc cgcagcctcg tgcgcggcgt gcggcgcctt
     9421 gtcgtgcagc ctctcgaggg cttgcagcgc cgccaggccg tcgatggccg cgtagtgcac
     9481 cttcagcagg atcgcgaaac tgccgcgcgg caggaactcg atattgtcca gcccctcgat
     9541 cacggtcgcc tcccacaacg gccgcgagcg gtcgacgggg cgcgcgtgga tccgcgccgc
     9601 catgatgcag agctggcgcc agtcacccgg cttgggcagc gccacgtggt tcacgtgcga
     9661 ctcgatgttg aagtgcccgt cgtgcaccca gtacggattg tccagaccga acggcacctc
     9721 ctgcacgcgg cagtgcagga acggtacctc gcgggcacgc tcggcgaaga cgttcatgat
     9781 gtcgcgcagg cgcacgcgcc cgccgggcgc cgtctgctgg gaataaaaca gcaacgcgct
     9841 caggtgcgcc gcgcagcgcg ggctttcctg ggtgagatac agccgatcca gggcgctcaa
     9901 ctggtgcatt cgcttctcct tctgttggcg gcgccgacga cgccgcgcaa ttctccgccc
     9961 gcagggccgg cgcgcgattg atcgaggtca accgcgcggc acggattcac ggtatcgatt
    10021 cggtggtgta gcgctcgacg cggacagcgt ccgaccagtg agcgcaatcc aggccggcct
    10081 tctgcttcag gcgcgccagg aattcgcgcg gctcgggcaa ctgctcccac accgagggca
    10141 ggaaggtgcc gcgcgcgtgg cgctcgctga ggatcagccc gtcgatgccg ggacgcagct
    10201 ggccgagcag ctcttcctcg gagtcgaaca cgagcagctc cggcgggctc agcaccgaga
    10261 tatgcagttc gatgcgctcg aactcctcgc gagcgagcgc ggggaagcgc ggatcgccaa
    10321 aggccgcggc atgcgccatc tcggccacct cgagaaccag cgcgcgccgt gcctccagcg
    10381 tacccatgca cccgcgcagg cgcccggcca ggttcagcgt gacaaagctc gctcgtggtt
    10441 gggcgagcgc aggcgcgaac gcctcgacct ccacccgcag gcgctcgcca tcgagccccg
    10501 cgcggatcgc ctggcccgcc agcgccagca gcgtggcgcg ctcggccggg ccgaaggcgc
    10561 actcagccga cgagccaggc gccatagccc accacgcgct cgcgatcgcc cgccgtatca
    10621 ccggaattgc gcaggtccac cgtggtcacg cgcaggccgc ggcggcgcgc caggcacagc
    10681 agcccgccca gcggcacgcg cccgcaggcc gagtcgcgcc ccaggtcctc gaagcgcagc
    10741 gcctcgatag ccgcgcaggt cgtgcggtcc aggcgctgcg cggtctcgta atcgtaatag
    10801 tgactgaggt ccgtgctgac gacgaccagc gtgcccgggc cgtcccagac gcgctcgagc
    10861 acctcggcaa cctcttcggc cgatgcctcg ccgaccacca ggggaaccag ggagaaatca
    10921 cccagcactt cctgcaggaa cggcaggtgc acctccaggc tgtgctcgtg ggcgtgggcc
    10981 gcatcgaggc agaccacctg cgccagcgcc gacagcgccg cgatcgccgc gtgatcgagc
    11041 tcgatgcgtc cgaacggggt ctcgtagctc tcgcagctcg gcaccgaaag accgtgaaac
    11101 gcaacccggt gcgagggacc gagcagcacg acgcgcttca cgcgaccgcg cgccggcgca
    11161 agccgggcat aggccgcggc ggccacgggc gccgaataca catagcccgc atggggcacg
    11221 atgatggcct tcgggatctc gccaccctgc ggcacgacgc tcaggaacgc ccgcacctct
    11281 tcatgcaggc tctcgggatc cgccggataa aacacccccg ccaccgctgg tttgcgcacc
    11341 gccctcatgg catgccctcc atggtcgagg gttccaaaat gcgccccttg ccgtgcattt
    11401 tcaagcaccg cgtgctgaac tcgcccgcga tcctgcgctc caatggcgca tgaccggcag
    11461 cgaacgtttc gaaggctcct ttcccacccg gcactggcac acgctctccg acgggcgcgt
    11521 gcagtgcgat gtctgcccgc gcttttgcaa gttgcacccg gggcagcgcg gattgtgctt
    11581 cgtgcgcggc aaccaggacg acgccatcgt gctgaccacc tggggacgct ccagcgggtt
    11641 ctgcgtcgac cccgtcgaga agaagccgct gaaccatttc ctgcccggca cgccgatcct
    11701 gtccttcggc accgccggct gcaacctggc ctgcaagttc tgccagaact gggacatcag
    11761 caagtcgcgc gaatgggaca cgctggccga tgccgcgacg cccgaaacga tcgcgcgcgc
    11821 ggcgcgggaa ctgggctgcc gcagcgtggc ctacacctac aacgaccccg cgatcttccc
    11881 cgagtatgcg atcgacgtcg ccgcggcctg ccgcgagctc gacatcaggt cggtggccgt
    11941 gaccgcgggc tacatgacgc cggcgccgcg cgcggagctg ttccgccaca tggacgccgc
    12001 caacgtcgac ctgaaatgct tcaccgagga tttctaccac aagctgtgcg gcgcgcatct
    12061 cgcctccatc ctggagacgc tgctctacct caggcacgag acgacggtgt ggctggagct
    12121 caccacgctg ctgatcccgg gcctgaacga ctccgatcgc gagatcgagg agatgacgca
    12181 gtgggtggtg gaaaacctcg gcccggacgt accgtggcac ttcacggcct ttcaccccga
    12241 ttggaagatg cgcgacatcc cgcccacccc gctcggcacg ctgctacgcg cgcatgcgat
    12301 cgcgcgcagg aacggcgtgc gctacgccta cacgggaaac gtgcacgacg aagcgcacgg
    12361 cgccacccac tgccacacct gcgacacgcg cctgatcggg cgtgacgggt accgcatcac
    12421 cgactggaac ctcggacccg gcggcacgtg ccgcagctgc ggcagcgcgt gcgcgggcct
    12481 gttcgacgcc gacccgggca cctggggcgc caggcgtcag ccggtgcgac tgctcgacta
    12541 ccacgtctga ggccggtctg gcgtgagttc tgtgtgggtt gagcttcagc ccgacaaggg
    12601 ttcggcgtgg gtcaggctgc agagggtgtc gcaaaaccat ctgtgccctg gatagcgata
    12661 gcggcccgac aatatcccgc gtatcgcgag ccatcggcgc tcaagcagcc atcggtgccg
    12721 cggacacgcc gtgaatcccc ctccggggcc cggcccgaaa tcg
//
LOCUS       DAORUQ010000096        12642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_4971_length_12642_cov_5.731390, whole genome shotgun sequence.
ACCESSION   DAORUQ010000096 DAORUQ010000000
VERSION     DAORUQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4971_length_12642_cov_5.731390"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLW13_09615"
     CDS             <1..282
                     /locus_tag="PLW13_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQY70675.1"
                     /translation="ELARPQQTVVFYMGLVGLPLICERLIAHGMPESTPVALVQQGTT
                     PAQRVLVSTLAAMPGRLRAAQFHAPTLAIVGSVVSLHERLSWFQPAAET"
     gene            418..660
                     /locus_tag="PLW13_09620"
     CDS             418..660
                     /locus_tag="PLW13_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70676.1"
                     /translation="MSIDIPRVLPIVTPPAAVLDDELRVKQSARVDPIPSDPGKPRVE
                     RRVVPERRTRTEQRRAALELRARRDRRTQRGIDVNA"
     gene            678..2033
                     /gene="gorA"
                     /locus_tag="PLW13_09625"
     CDS             678..2033
                     /gene="gorA"
                     /locus_tag="PLW13_09625"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HQY70677.1"
                     /translation="MNTFDFDLFVIGAGSGGVRAARISAGYGARVAVAEERYVGGTCV
                     NVGCVPKKLFVYAAHYAEDFHDAAGFGWALGAAAFDWPRLRDNKDREIARLNGIYRNL
                     LDSKGVTLIEGRARVLDAHSVAVGQRHYRARHILIATGAWPFVPDFPGREHVITSNEA
                     FHLERLPRRAVVVGGGYIAVEFAGILNGLGVETALLYRGPLFLRHFDRDLRETLAEEM
                     PKKGVTLRFDTTVQAVEKRPDGSFELQLGDGSRLATDLVLYATGRRPLVQDLGLEACG
                     VELREDGSIVVDDYYQSTVPSIHAIGDVIGGPELTPLATAQGMALASTLFLGDKRSVS
                     LENLPTAIFSQPAVGTVGLSEEQARARYPAVLVYRTRFRPLKHTLSGNPEQTFMKLVV
                     DGESDRVLGVHMVGPEAGEIVQGMAVALQAGATKRVFDATLGIHPTSAEEFVTLREPV
                     R"
     gene            complement(2064..4178)
                     /locus_tag="PLW13_09630"
     CDS             complement(2064..4178)
                     /locus_tag="PLW13_09630"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQY70678.1"
                     /translation="MQQSRTLRAPVVVRAILLLSLACPGLLQAGAEQKPLGYDRTQSL
                     TTLDIIERLGRHHYARVQIDDALGERLLENYLDNLDPGRNVFLAADIEEFRKLRHQLD
                     DQLLAGDVSAGFAIYARYRERMDARLGHVTENLHATIRAMDFSKDEVLLTDRDKLDWP
                     ADEQEAEELWHKQLKSSVLGLKLAGKEMGAIEDLLGKRYKDQLRRMAQVNSEDVYQLY
                     MNSFTELFDPHTNYLSPRSSENFNMNMRLSLEGIGAVLQQDDEFTKVARLVPAGPAAK
                     QGQLRQSDRIVAVGQDGGGEMVDVVGWRLDDVVDLIRGKKGSTVRLEVVRGSGASEEH
                     HFISIVREQVKLEEQAAQSRVLEIPYEDHTRKIGVIDIPAFYADFEAMQRGDPNYRST
                     TRDVARLLAELQEQQVDGVIIDLRENGGGSLQEANALTGLFIESGPTVQIRHSNERVE
                     RKQKFRDDSFYAGPLLVLINRLSASASEIFAGAIQDYERGLVVGSPSFGKGTVQSLSP
                     LNHGQLKMTESKFYRISGESTQHRGVVPDIALPSIYNLNEVGESALDNALPWDRIAPV
                     RHRNYLDLGSILPTLRERHEARTSKDPDFAYLRDEIAFAEKLDAVTSVSLSEPVRLAE
                     RQQQREEELKLENRRRVAKGEQPLASVEELEKDKQAAEDEAAVTAQDTTRADDEPDVL
                     LTEAGNILLDAIQLNQRIAVSN"
     gene            4318..5361
                     /locus_tag="PLW13_09635"
     CDS             4318..5361
                     /locus_tag="PLW13_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HQY70679.1"
                     /translation="MRLGLNIGYSGKRATAPVELVQQAEAAGFDSVWVAEAYGSDAVS
                     VAAWLLARTTRIRVGTAIMQIPARTPANAAMTAMTLAQLSGDRFLVGLGASGPQVVEG
                     WHGVPYGKPVTRMREYIQIMRKIMEREAPVEFDGEIYQLPWRGPGATGLGKPLKSILE
                     ATPGIPIYCASFTPAGLAAAAEVADGVFPIWLSPEQGGLIEDHLAPGFARRSDGRTRA
                     DFDVAPTLIVVVGDDVAACRRPVKEMLALYVGGMGARDKNFYNRYARDLGYEAAAVAI
                     QDLFLAGRKEEAVQAVPDAFVDEIALVGPLARIGERASAWKRLGAEGKVGTLILGMRQ
                     PEYLGAIAGILLG"
     gene            5358..7118
                     /locus_tag="PLW13_09640"
     CDS             5358..7118
                     /locus_tag="PLW13_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQY70680.1"
                     /translation="MSVPASMTVPVIRSVEDLERLHFGHAAESEAKLVSTLAESGLVE
                     AEALDRALAYRHKNGTSLLQAIDDLGVLDTSHRPAVVASKLGIPVARIEEFTVEPAVL
                     RAIPVQVALKHRVFPLARHDGNLVVAVSDPADLEAINTITFAVGQRVELVVAPLEDIN
                     RLIDRHFLILEEQNLIAEIGDDAREIRPQHPAEEHLHDIEKRAQEKPVVRLVDAIIRR
                     AVRMRASDINIRPTEHGAEVYYRIDGEMVWQRLLTPDLTAPIVCRIKIMAGMNIAERR
                     LPQDGHAGLVEAGQAVDLRVSVIPMVTGESVVIRVLDKSQGLIGLEQLGIAAEDMRRL
                     ERILQHSYGIFLVTGPTGSGKSTTLYAVINERLKNNPHIITVEDPVEYHISGVEQIQI
                     KPTIGYTFAEALRHILRHDPDEILIGEMRDFETSEIAIKAALTGHFVMSTLHTNDAAS
                     AITRLQDMGLESYLVSSTVVGIMAQRLVRRICSACRTEDAGSAELRDYFGLPAGETFY
                     RGAGCGKCFDTGYKGRAMVGEIIEVTSALREMITRKPGAEEIRTLAIREGMTPLTQNA
                     LAMARAGITTLDEVFSVKLE"
     gene            7127..8767
                     /gene="gpmI"
                     /locus_tag="PLW13_09645"
     CDS             7127..8767
                     /gene="gpmI"
                     /locus_tag="PLW13_09645"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQY70681.1"
                     /translation="MLSRSSIFPGVAGPVVTVVMDGIGQRGATAGNAVADAHTPTLDR
                     LFARYPHTLLKAHGTAVGMPSDEDMGNSEVGHNALGSGQVYAQGAALVNDAIASGSLF
                     AGAAWGEIVANVLASGGTLHLLGLFSDGNVHSHIEHLKALVTAARGAGVGRVRIHALL
                     DGRDVPATSALDYVLPFEQFLAGLRSEAFDARIASGGGRMHITMDRYEADWDMVARGW
                     ATHVLGEGRRFASAAEAIATLRGEKSGIGDQDLPAFVIATEGAPLGPIVDGDSVVFFN
                     FRGDRAIEISRAFTEEEFTPFARARVPRLCYAGMLQYDGDLQIPRRFLVAPPAIRDTM
                     GEYLSGAGVSQFAISETQKFGHVTYFWNGNRSGRFDEDLERWLEIPSDRVPFEERPWM
                     KAAEITDALIAELKTGRHRVARVNYANGDMVGHTGNYYAAVMAVEAVDLALARLLPVI
                     DALGGVALVTADHGNAEEMFEIDRKSGAPSRDAAGHTRPKTSHTLNPVPLVLYDNQSG
                     GRLSVVEGGLANVAATTLELLGFAAPGMWRPSLLRVAQ"
     gene            complement(8772..9515)
                     /locus_tag="PLW13_09650"
     CDS             complement(8772..9515)
                     /locus_tag="PLW13_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70682.1"
                     /translation="MSRAVLITGASSGIGEALAYEFARRGYRLAVLARRVAALQELEI
                     ALRAAGSPQVVVHALDLRDPAEIEPALSACAEMLGGLDIVVANAGIAPRTPVGRGALA
                     DLQETIAVDLTGACATVDAAVRHFRARGGGQVVGITSIARYRGLPNFGAYSAAKEGLH
                     RYLQALRAESWHEQITVTELAPGYIDTPLNRAMRSRPFVIDVEKGARLMAGLIERRVA
                     YATVPALPWRIAGRLLQLLPTRLLAPRGR"
     gene            complement(9512..>11327)
                     /locus_tag="PLW13_09655"
     CDS             complement(9512..>11327)
                     /locus_tag="PLW13_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQY70683.1"
                     /translation="HCATCHANPATRSPTLASLQQMPLARLLSAMEFGKMQVQAAALT
                     AQQRLALGQWLAAPEDARRDAWIARHACTHPPGEIPLSGAQNWGFGAHNTRFLREGVA
                     ITAANIGQLELAWSLALPQVTDMRSQPVAAGSLLFLGTQTGNLLALDQASGCVYWASR
                     AVGGIRSALTLAATPDGVATLFFADDLGTVYAVDARTGIARWHVDVRLFPSSVVSGSP
                     SYHDGRLFVPLSSFEVAAAGMPTHACCRSHGGVRALDARSGKVLWTWHSTPPAEPTGK
                     SAAGVQRWGPSGASVWSTPTIDAKRGLLYVGTGQNFSRPATATSDAVIALGIDDGRPR
                     WQFQALADDVWNGACQLAGPNCPEHPGPDWDIGASVVLSTSPAGRDTLLAGQKSGELF
                     ALDPDARGKLLWRKRLSQGTPNGGNSGIHWGMASDGTHVFAPVSDPERELPGYTPRPG
                     VYALRIVDGSLLWEHPVTRGCAVDPADVPLAGLAAMREGATGAARSPWPACSFFYAHS
                     AAAVLANDVVYAGALDGRLRALDAATGRELRTLETARAFRAGNGLDGHGGSIDVGGVL
                     PHGRFLFVVSGYGMFRQMPGNMLLAWALPGTPAREEAP"
     assembly_gap    11328..11427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11573..>12642
                     /locus_tag="PLW13_09660"
     CDS             11573..>12642
                     /locus_tag="PLW13_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70684.1"
                     /translation="MSSGTIGPLLDADEFFNHQIVDTFATVSQSDYSWTEKVCGMAAA
                     RDGSLSIGFGFGKYPNRNVVDAYGGASRGVEQWTVRGSRALASDPNSIDVGPLKYAVI
                     EPLKQVRVVLEPNATQPVAFDLVLEGIVPCMLEEREDRRNLHGFRHTANQIRYHQTGS
                     ARGWVEVAGVRTEVTPETWIMTRDHSWGVRPDVGVRIPDLAPDPMDGLVPKALAIWNP
                     LFFRKPDGSTYAFHQYYLSYSGPGFKHERVQGGYEYADGRRETLVGITPELRFDPKNK
                     RLLGGEFRVQMADGSERRLTAEAISDTGFHLGAGLYHGFDGQHHGSWRGALHVEGEYF
                     ADCSTPESVARLNQFRDCIIRV"
BASE COUNT         1893 a         4273 c         4458 g         1918 t
ORIGIN      
        1 gagctggcgc ggccacagca gaccgtggtg ttctacatgg ggctggtcgg gctgccgctg
       61 atctgcgagc ggctgatcgc gcacggcatg ccggagtcca cccccgtggc gctggtgcag
      121 cagggcacca cgcccgcgca gcgcgtactg gtatcgaccc tcgccgcgat gcccgggcgc
      181 ttgcgcgcgg cgcaattcca cgcgcccacg ctggcgatcg tgggctcggt cgtgagcctg
      241 cacgagcgcc tgagctggtt ccaaccggcg gccgaaacct agaatcaact ttaagttttt
      301 tagataagat ccggaacctg ttgatttcgt tgaattaaca tcaagaaact tctcaagttc
      361 gctcccgcgc tgccgattca atactgccca agcagtcttg cctcacggag tccagccatg
      421 agcatcgaca tcccccgcgt acttcccatc gtgaccccgc cggcagccgt gctcgacgac
      481 gaactgcggg tgaagcagtc ggcgcgggtc gacccgatac cgtccgaccc gggcaagccg
      541 cgtgtcgagc gccgggtggt gccggagcgg cgcacccgca ccgagcagcg gcgcgcggcg
      601 ctggagttgc gcgcgcggcg cgaccggcgc acccagcgcg gcatcgacgt gaatgcctga
      661 gtccacggac ggatgccatg aatacctttg atttcgacct cttcgtgatc ggcgccggat
      721 cgggcggggt gcgcgccgcc cgcatctcgg ctggctacgg tgcccgcgtc gcggtggccg
      781 aggagcgcta cgtgggcggc acctgcgtca atgtcggctg cgtgcccaag aaactgttcg
      841 tgtatgccgc gcattacgcg gaggatttcc acgacgccgc cgggttcggc tgggcgctcg
      901 gggcggcagc cttcgactgg ccgcggctgc gcgacaacaa ggatcgcgag atcgcgcgcc
      961 tcaacgggat ctaccgcaac ctgctcgaca gcaagggggt cacgctgatc gagggccgcg
     1021 cgcgcgtgct cgacgcccac tcggtcgcgg tggggcagcg gcactaccgc gcgcgccata
     1081 tcctgatcgc caccggggcc tggcctttcg tgccggactt cccggggcgc gagcacgtga
     1141 tcacctcgaa cgaggcattt cacctcgagc gcttgccgcg gcgcgcggtg gtggtgggcg
     1201 gcggctacat cgcggtggag ttcgcgggca tcctgaacgg cctgggggta gagaccgcgc
     1261 tgctgtatcg cggcccgctg ttcctgcgcc atttcgaccg cgacctgcgc gagacgctgg
     1321 ccgaggagat gccgaagaag ggcgtgacgc tgcgcttcga caccacggtg caggcggtcg
     1381 aaaagcgtcc ggacggcagt ttcgagctgc agctgggcga cggctcgcgg ctggcgacgg
     1441 acctggtgct gtacgcgacc ggccgccgcc cgttggtgca ggatctgggc ctggaggcct
     1501 gcggcgtgga gctgcgcgag gacggttcga tcgtcgtgga cgattactac cagagcacgg
     1561 tgccctcgat ccacgccatc ggcgatgtca tcggcggccc ggagctgacg ccgctcgcga
     1621 ccgcgcaggg aatggcgctc gcgagcacgc tgttcctggg cgacaagcgc agcgtcagcc
     1681 tcgagaacct gccgaccgcg atattctcgc agccggcggt gggcaccgtg ggcctgagcg
     1741 aggagcaggc ccgcgcccgc tatcccgccg tgctggtgta ccgcacccgc ttccggccgc
     1801 tcaagcacac gctgagcggc aatcctgaac agaccttcat gaagctggtg gtagatggcg
     1861 agagcgaccg cgtgctgggc gtgcacatgg tggggccgga ggccggggag atcgtccagg
     1921 gcatggcggt ggcgctgcag gcgggtgcca ccaagcgcgt gttcgacgcc acgctcggca
     1981 tccatccgac ctcggccgag gaattcgtga ccctgcgcga gccggtgcgc tgagaacggg
     2041 ggggcttagc cccccgtgag ggttcagttg ctgacggcga tgcgctggtt cagctgtatc
     2101 gcgtcgagca ggatgttgcc cgcttcggtc agcagcacgt ccggctcgtc gtcggccctg
     2161 gtggtgtcct gcgcggtaac cgccgcctcg tcctcggcgg cctgcttgtc cttttccagc
     2221 tcttcgaccg aggcgagcgg ctgctcgccc ttcgcgacgc gccgccggtt ctcgagcttc
     2281 agttcttcct cgcgctgctg ctggcgctcg gcgaggcgga ccggctcgct gagcgagacc
     2341 gatgtcacgg cgtcgagctt ctcggcgaag gcgatctcgt cgcgcaggta ggcgaagtcc
     2401 gggtccttgc tggtgcgggc ctcgtggcgc tcgcgcagcg tcggcaggat cgagccgagg
     2461 tcgaggtagt tgcggtggcg caccggggcg atgcggtccc acggcagcgc gttgtcgagc
     2521 gcgctctcgc cgacctcgtt caggttgtag atcgagggca gcgcgatgtc gggcaccacg
     2581 ccgcgatgct gcgtgctctc gcccgagatg cggtagaact tcgactcggt catcttgagc
     2641 tggccgtggt tcagcgggct cagggactgc accgtgccct tgccgaagga gggcgagccc
     2701 accaccagcc cgcgctcgta gtcctggatc gcgccggcga agatctccga ggccgaggcg
     2761 ctcaggcggt tgatcagcac cagcagcgga ccggcgtaga agctgtcgtc gcgaaacttc
     2821 tgcttgcgct cgacgcgctc gttactgtga cggatctgca ccgtcgggcc cgactcgatg
     2881 aacaggccgg tgagcgcatt ggcttcctgc agcgagccgc cgccgttctc gcgcaggtcg
     2941 atgatcacac catcgacctg ctgctcctgc agttcggcga gcagtcgcgc cacgtcgcgc
     3001 gtggtgctgc ggtaattcgg gtcgccgcgc tgcatggcct cgaaatcggc atagaaggcc
     3061 gggatgtcga tcacgccgat cttgcgcgtg tggtcctcgt aggggatctc cagcacgcgg
     3121 ctctgcgccg cctgctcctc gagcttgacc tgctcgcgca cgatgctgat gaagtgatgc
     3181 tcctcgctgg cgccgctgcc gcgcacgacc tccaggcgca cggtgcttcc cttcttgccg
     3241 cggatcaggt cgaccacgtc atcgaggcgc cagccgacca catcgaccat ctcgccgcca
     3301 ccgtcctggc cgacggcgac gatccggtcg gactggcgca actggccctg cttggccgcc
     3361 ggcccggccg gcaccaggcg agcgaccttg gtgaactcgt cgtcctgctg cagcaccgcg
     3421 ccgatgccct ccagcgacag gcgcatgttc atgttgaaat tctcggagct gcgcggcgag
     3481 aggtagttgg tgtgcgggtc gaacagctcg gtgaacgagt tcatgtagag ctggtagaca
     3541 tcctcgctgt tgacctgcgc catgcggcgc agctggtcct tgtagcgctt cccgagcagg
     3601 tcctcgatgg cgcccatctc cttgcccgcg agcttcagcc cgagcacgct ggacttcagc
     3661 tgcttgtgcc agagctcctc cgcctcctgc tcgtcggccg gccagtcgag cttgtcgcgg
     3721 tcggtcagca gaacttcgtc cttgctgaag tccatggccc ggatcgtcgc gtgcagattt
     3781 tccgtcacgt gcccgagccg tgcgtccatg cgctcgcggt agcgcgcgta gatcgcgaag
     3841 ccggcactga cgtcgccggc cagcagctgg tcatcgagct ggtggcgcag cttgcggaac
     3901 tcctcgatgt cggcggcgag gaacacgttg cgccccgggt cgaggttgtc caggtaattc
     3961 tcgagcagcc gctcgcccag ggcgtcgtcg atctgcacgc gcgcgtagtg gtgccggccg
     4021 agacgttcga tgatgtcgag ggtggtcagg ctctgggtgc ggtcgtagcc gagcggcttc
     4081 tgctcagcgc cggcctgcag cagcccggga caggccagcg acagcaacag gatggcacga
     4141 acgacgaccg gggcgcggag agtgcgggat tgctgcataa ggacttggcg aatgggctcg
     4201 gtagcaatga cttcgcggcc attattaccc ataatcaacg cgggatacat ggtcattaac
     4261 ggcccgggaa acgtgaattc tcggcgccgc agcgtcaacg aagggggaaa tcggcgcatg
     4321 cgcctcggtc tgaacatcgg gtattcggga aagcgggcca cggcaccggt cgagctggtg
     4381 cagcaggccg aggcggcggg attcgattcg gtctgggtgg cggaagccta cggctccgac
     4441 gcggtttcgg tggcggcctg gctgctggcg aggaccacgc gcatccgcgt aggcaccgcg
     4501 atcatgcaga ttccggcccg cacgccggca aacgccgcca tgaccgcgat gacgctggcg
     4561 caactctccg gcgatcgatt cctggtcggg ctcggcgcct cgggcccgca ggtggtcgag
     4621 ggctggcacg gcgtgcccta cggcaagccg gtcacgcgca tgcgcgagta catccagatc
     4681 atgcgcaaga tcatggagcg cgaggcgccg gtggagttcg acggcgagat ctaccagctg
     4741 ccctggcgcg gtcccggcgc cacgggcctc ggcaagccgc tgaagtccat cctcgaggcg
     4801 acgccgggga tcccgatcta ctgcgcgagc ttcaccccgg ccggcctcgc ggcagcggca
     4861 gaagtcgccg acggcgtgtt cccgatctgg ctctcgccgg agcagggcgg gctgatcgag
     4921 gatcacctgg ccccgggctt cgcgcggcgc agcgacggca ggacgcgcgc ggacttcgat
     4981 gtggcgccca cgctgatcgt cgtggtcgga gacgacgtcg cggcctgccg gcgtccggtc
     5041 aaggagatgc tggcgctcta cgtggggggc atgggcgcgc gcgacaagaa cttctacaac
     5101 cgctacgcgc gggatctcgg ctacgaggcc gcggcggtcg cgatccagga cctgttcctt
     5161 gccggacgca aggaagaagc cgtgcaggcg gtccccgacg cgttcgtcga cgagatcgca
     5221 ctggtcggtc cgctggcgcg cattggcgag cgcgcgagcg cgtggaagcg cctcggcgcc
     5281 gagggcaagg tcggcacgct gatcctcggc atgcgccagc cggagtatct cggggccatc
     5341 gccgggatcc tgctgggatg agcgtgccgg caagcatgac cgtgccggtg atccgcagcg
     5401 tggaggacct cgagcggctg catttcgggc atgccgccga gagcgaggcg aagctcgtga
     5461 gcacgctggc cgagtccggg ctggtcgagg ccgaggcgct cgaccgggcg ctggcgtacc
     5521 ggcacaagaa cggcacctcg ctgctgcagg ccatcgacga cctcggcgtg ctcgatacct
     5581 cgcaccggcc tgcggtggtc gcctcgaagc tcggcattcc ggtggcgcgc atcgaggagt
     5641 tcacggtcga acccgccgtg ctgagggcca tcccggtgca ggtggcgctg aagcaccgcg
     5701 tgtttccgct ggcgcgccac gacggcaacc tcgtggtggc ggtgtcggac ccggccgatc
     5761 tggaggcgat caacacgatc accttcgcgg tcgggcagcg cgtggaactg gtggtggcgc
     5821 cgctggagga catcaaccgc ctcatcgacc gccacttcct gatcctcgag gagcagaacc
     5881 tgatcgccga gatcggcgat gacgcgcgcg agatccgccc gcagcacccg gccgaggagc
     5941 acctgcacga catcgagaag cgcgcgcagg aaaaacccgt ggtgcgcctc gtcgatgcga
     6001 tcatccgccg cgccgtgcgc atgcgcgcct cggacatcaa catccgcccg accgagcacg
     6061 gcgccgaggt ctactaccgc atcgacggcg agatggtgtg gcagcgcctg ctcacgccgg
     6121 acctgaccgc gcccatcgtc tgccgcatca agatcatggc cggcatgaac atcgccgagc
     6181 gccggctgcc gcaggatggc cacgccggcc tggtcgaggc cgggcaggcc gtcgacctgc
     6241 gcgtctcggt gatcccgatg gtcacgggcg agtcggtggt gatccgcgtg ctcgacaaga
     6301 gccaggggct gatcgggctg gagcagctcg gcatcgccgc cgaggacatg aggcgcctcg
     6361 agcgcatcct gcagcacagc tacggcatct tcctcgtgac cggtcccacc ggttcgggca
     6421 agagcaccac gctgtatgcg gtgatcaacg agcgcctcaa gaacaatccg cacatcatca
     6481 cggtcgagga tccggtcgag taccacatca gcggtgtcga gcagatccag atcaagccga
     6541 cgatcggcta caccttcgcc gaggcgttgc gccacatcct gcgccacgac cccgacgaga
     6601 tcctgatcgg ggagatgcgc gacttcgaga cctcggagat cgcgatcaag gccgcgctga
     6661 ccggtcactt cgtgatgagc acgctgcaca ccaacgatgc ggccagcgcg atcacgcgcc
     6721 tgcaggacat ggggctcgag tcctacctgg tgagctccac ggtggtcggc atcatggcgc
     6781 agcgcctggt gcggcgcatc tgcagcgcgt gccgcaccga ggacgcgggc agcgcggagc
     6841 tgcgcgacta cttcggtctg ccggccgggg agaccttcta ccgcggcgcc ggctgcggca
     6901 agtgcttcga caccggctac aagggacgcg cgatggtcgg ggagatcatc gaggtcacca
     6961 gcgcgctgcg cgagatgatc acgcgcaagc cgggcgccga ggagatccgc acgctggcga
     7021 tccgcgaggg catgacgccc ctgacgcaga acgcgctggc gatggcgcgc gccggcatca
     7081 ccacgctcga tgaagtgttc tcggtcaagc tcgaataagg ggttccatgc tttccaggtc
     7141 cagtattttt cccggcgtgg ccgggccggt ggtcacggtc gtgatggacg gcatcgggca
     7201 gcgcggcgcc acggcgggca atgccgtggc cgacgcgcac accccgacgc tcgaccggct
     7261 gttcgcgcgt tatccgcaca cgctgctgaa ggcccatggc accgccgtcg gcatgccgag
     7321 cgacgaggac atgggcaatt ccgaggtcgg gcacaacgcg ctcggctccg ggcaggtcta
     7381 tgcgcagggc gcggcgctgg tcaacgacgc catcgccagc ggcagcctgt tcgccggcgc
     7441 ggcctggggc gagatcgtgg ccaacgtgct ggccagcggc ggcacgctgc atttgctggg
     7501 gctgttctcc gacggcaacg tgcattcgca catcgagcac ctgaaggcgc tggtcacggc
     7561 ggcgcgcggc gccggcgtgg gccgcgtgcg catccatgcg ctgctcgacg ggcgcgacgt
     7621 gcctgcgacc tcggcgctcg actacgtgct gcccttcgag cagttcctgg ccggcctgcg
     7681 cagcgaggcc ttcgatgcgc gcatcgccag cggcggcggg cgcatgcaca tcacgatgga
     7741 tcgctacgag gcggactggg acatggtggc gcgcggctgg gccacgcacg tgctcggcga
     7801 gggacgccgc ttcgccagcg ccgccgaggc gatcgcgacc ttgcgcggtg aaaagtccgg
     7861 tatcggcgac caggacctgc cggccttcgt gatcgccacc gagggcgcgc cgctcggtcc
     7921 gatcgtcgac ggcgactcgg tggtcttctt caatttccgc ggcgaccgtg ccatcgagat
     7981 ctcgcgcgcc ttcaccgagg aggagttcac gcccttcgcg cgcgcccggg tgccgcggct
     8041 ctgctacgcc ggcatgctgc agtacgacgg cgacctgcag atcccgcgcc gcttcctggt
     8101 cgcgccgccc gcgatccgcg acaccatggg cgagtacctg agcggcgccg gcgtctcgca
     8161 gttcgcgatc tcggagacgc agaagttcgg ccacgtcacg tatttctgga acggcaaccg
     8221 cagcgggcgt ttcgacgaag atctcgagcg ctggctcgag atcccctccg accgcgtgcc
     8281 cttcgaggag cggccgtgga tgaaggcggc cgagatcacc gatgcgctga tcgcggagct
     8341 gaagaccggc cgccaccgcg tggcgcgcgt caactacgcc aacggcgaca tggtcggtca
     8401 caccggcaac tactacgccg cggtgatggc ggtggaggcg gtggatctcg cgctggcgcg
     8461 gctgctgcct gtgatcgatg cgctcggtgg cgtggcgctg gtcacggccg accacggcaa
     8521 cgccgaggag atgttcgaga tcgaccgcaa gagcggcgcg cccagccgcg atgccgccgg
     8581 tcatacgcga cccaagacca gccacacgct gaacccggtg ccgctggtgc tctacgacaa
     8641 ccagagtggc gggcggttga gcgtggtgga gggcgggttg gcgaacgtgg cggccaccac
     8701 gctggagctg ctgggattcg cggcgccggg catgtggcgg ccgagcctgt tgcgcgtggc
     8761 gcagtgagct ttcagcgccc gcgcggcgcg agcaggcgcg tcgggagcag ttgcagcagc
     8821 cggcccgcga tgcgccacgg cagcgcgggc acggtggcgt aagcgacgcg gcgctcgatc
     8881 aggccggcca tcaggcgcgc gcctttttcc acgtcgatca cgaacgggcg cgagcgcatg
     8941 gcgcggttca gtggcgtgtc gatgtaaccg ggcgcgagct cggtcaccgt gatctgctcg
     9001 tgccagctct cggcccgcag cgcctgcagg tagcggtgca ggccttcctt cgcggcgctg
     9061 taggcgccga aattcggcag cccgcgatag cgtgcgatcg aggtgatacc caccacctgc
     9121 ccgccgccgc gggcgcgaaa gtggcgcacc gcggcatcga cggtggcgca ggcgccggtg
     9181 aggtccaccg cgatggtttc ctgcagatcg gcgagcgcgc cgcgcccgac cggtgtgcgc
     9241 ggcgcgatac ccgcgttggc caccacgatg tccagcccgc cgagcatctc ggcgcaggcg
     9301 ctgagcgcgg gctcgatctc ggcgggatcg cgcaggtcca gcgcgtgcac caccacctgc
     9361 ggcgagcccg cggcgcgcag cgcgatctcc agttcctgca gcgcggccac gcgccgcgcc
     9421 agcaccgcca ggcggtagcc gcggcgcgcg aactcgtatg ccagcgcctc gccgatgccg
     9481 ctcgaggcgc cggtgatcag cacggcccgg ctcatggcgc ctcctcgcgc gccggcgttc
     9541 cgggcagcgc ccaggcgagc agcatgttgc ccggcatctg ccggaacatg ccgtagccgg
     9601 agaccacgaa caggaaacgc ccgtgcggca gcacgccgcc gacgtcgatc gaaccgccgt
     9661 gcccgtcgag gccgttgccg gcgcggaacg cgcgcgcggt ctcgagcgtg cgcagttccc
     9721 gtcccgtggc ggcatccagc gcgcgcagcc tgccgtcgag tgcgccggcg tagaccacgt
     9781 cgttggccag caccgcggcg gcggaatgcg cgtagaagaa ggagcaggcg ggccacggcg
     9841 agcgcgcggc gcctgttgcg ccctcgcgca tcgcggccag cccggccagc ggcacgtcgg
     9901 ccggatcgac cgcgcagccg cgcgtcaccg ggtgctccca cagcaggctg ccgtcgacga
     9961 tgcgcagggc atagacgccc ggacgcggcg tgtagccggg cagttcccgt tccggatcgg
    10021 acaccggcgc gaacacgtgc gtgccgtcgc tcgccatccc ccagtggatg ccgctgttgc
    10081 cgccgtttgg tgtgccctgg ctgaggcgct tgcgccacag cagcttgccc cgcgcgtcgg
    10141 ggtccagcgc aaacagctcg ccggatttct gccccgccag cagcgtgtcg cggcccgcgg
    10201 gcgaggtgct cagcaccacg gaggcaccga tgtcccagtc cgggccggga tgctccgggc
    10261 aattggggcc ggcgagctgg caggcgccgt tccacacgtc gtcggcgagc gcctggaact
    10321 gccagcgcgg cctgccgtcg tcgatgccga gcgcgatgac cgcgtcactg gtcgcggtcg
    10381 ccgggcgcga gaaattctgg ccggtgccga cgtagagcag gccgcgcttg gcgtcgatgg
    10441 tcggggtgct ccacaccgag gcgcccgagg ggccccagcg ctgcacgccg gcggcgctct
    10501 tcccggtggg ctcggccggc ggcgtgctgt gccaagtcca gagcaccttg cccgatcgcg
    10561 catccagcgc gcgcacgccg ccgtgcgatc ggcagcatgc gtgcgtcggc atgccggcgg
    10621 ccgcgacctc gaaggaggac agcggcacga acaggcgccc gtcgtggtag ctcggcgagc
    10681 cgctcacgac cgagctcggg aacaggcgca catcgacgtg ccagcgcgcg ataccggtgc
    10741 gtgcatcgac ggcgtagacc gtgccgaggt cgtcggcgaa gaacagcgtc gccacgccgt
    10801 cgggcgtggc ggccagcgtc agcgcggagc ggatgccgcc gacggcgcgc gacgcccagt
    10861 acacgcagcc gctcgcctgg tccagcgcca gcaggttgcc ggtctgcgtg cccaggaaca
    10921 gcaggctgcc cgccgcgacc ggctgcgagc gcatatcggt gacctgcggc agcgccagcg
    10981 accacgccag ttccagttgc ccgatgttcg ccgcggtgat cgcaacgccc tcgcgcagga
    11041 agcgcgtgtt gtgcgcgccg aaaccccagt tctgcgcgcc gctgagcggt atctcgccgg
    11101 gcgggtgcgt gcaggcgtgg cgcgcgatcc aggcatcgcg ccgggcatcc tcgggcgcgg
    11161 ccagccactg ccccagcgcc aggcgctgtt gcgccgtgag ggccgcggcc tgcacctgca
    11221 tcttgccgaa ctccatcgcg ctcagcagcc gcgccagcgg catctgctgc aggctcgcga
    11281 gcgtcgggga gcgcgtggcc gggttcgcat ggcaggtggc gcagtggnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg agaaataccc gtttcatgcg accccgcgaa
    11461 catgcacccg tcgtgcgccg attgtgggcg ccgtcgcggc gcacgcgcaa gcgttcgctc
    11521 ccgcgcttgg gctatgatgc gcgcgtttca ggcccccgca caggaagatc ccatgagttc
    11581 aggcaccatc ggaccgctgc tcgacgcgga cgagttcttc aatcaccaga tcgtcgacac
    11641 cttcgccacg gtgtcgcaga gcgactactc gtggaccgag aaggtctgcg gcatggcggc
    11701 ggcgcgcgac ggctcgctgt cgatcggctt cggcttcggc aagtacccca accgcaacgt
    11761 ggtcgacgcc tacggcggcg cgagccgcgg ggtcgagcag tggaccgtgc gcggcagccg
    11821 cgcgctcgcc tcggatccca acagcatcga cgtcgggccg ctgaagtacg ccgtcatcga
    11881 gccgctgaag caggtgcgcg tggtcctgga gcccaacgcc acgcagccgg tggccttcga
    11941 cctggtgctc gagggcatcg tgccctgcat gctcgaggag cgcgaggacc ggcgcaacct
    12001 gcacggcttt cgccacaccg ccaaccagat ccgctaccac cagaccggca gcgcgcgcgg
    12061 ctgggtggag gtggccggcg tgcgtacgga ggtcacgccg gagacgtgga tcatgacgcg
    12121 tgaccacagc tggggcgtgc gccccgacgt gggcgtgcgc atcccggatc tcgcgccgga
    12181 tccgatggac ggcctggtgc cgaaagcgct ggcgatctgg aatccgctgt ttttccgcaa
    12241 gcccgacggt tcgacctacg ccttccacca gtactacctc agctacagcg ggccgggctt
    12301 caagcacgag cgcgtgcagg gcggttacga atacgccgac gggcggcgcg agaccctagt
    12361 cggcatcacg ccggagctgc gtttcgaccc gaagaacaag cgactgctcg gcggcgagtt
    12421 ccgcgtgcag atggccgacg gcagcgagcg gcggctcacg gccgaggcga tctcggacac
    12481 gggttttcac ctcggcgccg ggctctacca cggcttcgac ggccagcatc acggctcgtg
    12541 gcgcggcgcg ctgcacgtcg agggcgagta cttcgccgac tgctcgacgc cggagagcgt
    12601 ggcgcggctg aaccagttcc gcgactgcat catccgcgtg ca
//
LOCUS       DAORUQ010000097        12335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_5168_length_12335_cov_6.527769, whole genome shotgun sequence.
ACCESSION   DAORUQ010000097 DAORUQ010000000
VERSION     DAORUQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_12335_cov_6.527769"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="lysA"
                     /locus_tag="PLW13_09665"
     CDS             complement(<1..1155)
                     /gene="lysA"
                     /locus_tag="PLW13_09665"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQY70685.1"
                     /translation="MTEAFHYRDGVLCAEDVALDDIAARFGTPVYVYSRAAIESQYDA
                     FAAALAAAPSHLICYAVKANSNLAVLNLLARRGAGFDIVSGGELRRVLMAGGDPGRIV
                     FSGVAKSHEDIVAALEARILCFNIESPAELERLQAVAAGHGTTAPISIRVNPDVDAQS
                     HPYISTGLRENKFGVSISEAPALYARAAALPNIRVTGIDCHIGSQLTALGPFVDALER
                     VLAMVDELAAQGITLDHIDVGGGLGVVYGEEHPPTPAEYAAALLARFGNRPHTLLTEP
                     GRAIVARAGALLTRVEYLKDNGDKRFAIVDAGMNDLIRPSLYGAWMGIEAVRPRHDIE
                     HAVYDVVGPVCESSDFLGKNRSLALAGGELLAVTGAGAYSFAMSSNYTSRC"
     gene            complement(1152..1268)
                     /locus_tag="PLW13_09670"
     CDS             complement(1152..1268)
                     /locus_tag="PLW13_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HQY70686.1"
                     /translation="MRLLILSTLLGVALLAGCGQKGPLFLPPETPPGQAGTP"
     gene            complement(1299..4172)
                     /locus_tag="PLW13_09675"
     CDS             complement(1299..4172)
                     /locus_tag="PLW13_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HQY70687.1"
                     /translation="MPTPAAISPADEGIDRKELRRLLTRFRAISAARLERVRGMLAPR
                     QRQFLDLLPLLLHIDHPALPGYVSQHTPCGISGYTPSATELAQARRLAISFSPQRLVT
                     PEPRLHAVYLMGSSGTLGHSDGSDLDIWICYPDELGSEELALLARKAVLLQQWAHGIG
                     LEAHLFLMNGEKFKRGQREALTGENCGTAQHYLLLDEFYRTAILLAGRLPAWWLIPPQ
                     HEQHYDEYCSHLMRKRYAGRAGLVDFGGIAELPAGEFLGAGIWQLYKAIDSPYKSVLK
                     LLLFEVYAADHPAAACLSADYKRAVCAGLEDIDELDPYVMVYRRIERHLLAREQPARL
                     EIVRRCLYYKAGKKLSQPPRAGIPSWQRLLMERLVAEWGWDRDKLALLDSRSHWKIQD
                     VEAERNELVKELTSSYRFLSEFARRGDAARAIEAREFNILGRRLFAAYERKAGKIDAV
                     NPGRAINVTENEISIGTLRVADGAPALWAAWAGVTRGSAMPAGRALRQAPSLSELLCW
                     CLVNGVIGERTRIAVRERADGLSAGEVQGMLRELREHVLGDHADVAPGDEAFASAARV
                     TDLLVFVNVAADPMRLAREQGLQRVSSRIDSLGYSALRENLVLNVETVARNSWGELVS
                     ARHAGVEGLLRCLRDFLPLAHGNAGLRVAVRCFCPSRAEPIATRVGEVFGDLAHHVQR
                     ERGASARYVLEIRNQLHVLQHRRGAGSSASADSLAALYEVLGEPQPQWSPLAIDRHAL
                     ERTPLPVIAARMTPWRCSVFSEARAEAIAVTIVDEHGSFLRAEYAGAREGTLLGAIAQ
                     FLESVRYRQNGSGGADPAVTRATEPSRFFRLVRAAGGDYSAQELPAPPGYRPGSFLEV
                     QAIAEAHEGEPLFTVYCDGVAFSQRELGAEFHARLGAYILGRRHEASAYPVYLTDLDL
                     SAVAPVADAPLQTVHYLRHRQRLEASINAALTGSDAIRDGLAI"
     gene            complement(4204..5607)
                     /gene="argH"
                     /locus_tag="PLW13_09680"
     CDS             complement(4204..5607)
                     /gene="argH"
                     /locus_tag="PLW13_09680"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQY70688.1"
                     /translation="MEKQQTSTLWGGRFSESTDAFVQRFTASVDFDRRLALHDIRGSL
                     AHAAMLARAGLLSAQEHASIVAGLGEIRREIEEGRFEWSVELEDVHMNIEARLTARIG
                     IAGKKLHTGRSRNDQVATDVRLYLRDEIDAIAAELTRLQQGLVELAAREAATIMPGFT
                     HLQTAQPVTFGHHMLAWNEMLERDYARLLDCRARMNVSPLGAAALAGTSYPIDREFTA
                     AELGFSAPTRNSLDSVADRDFAIEFCAFAALLMTHLSRFSEELVLWTSAQFGFVNLPD
                     RFCTGSSIMPQKKNPDVPELVRGKSGRVTGHLMSLLMLMKSQPLAYNKDNQEDKEPLF
                     DTIDTVRDSLHAFADMMPALTVNEATLREAARRGFATATDLADYLVRKGMPFRDAHEV
                     VGKAVAAGVATRTDLADMPLETLRGFSATIGADVFEVLSLEGSVSARDHIGGTAPGRV
                     AQAAAEARARLAARTAS"
     gene            5876..6694
                     /locus_tag="PLW13_09685"
     CDS             5876..6694
                     /locus_tag="PLW13_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY70689.1"
                     /translation="MQRLLRPASSLIDTSAASPVPAGTLIYRCNVCSSTCSVPVAALE
                     RDGGNCAVCDSNVRFRSLVHLLSLRLFGRSLAISAFPAQARHLRGIGMSDTGRYAELL
                     AQRTGYTNTFYHKAPRLDIQAPASGYLAAHDFVISSDVFEHVVPPVATAFTNLRALLK
                     PGGVLVFTVPFSLDSETVEHFPELHEFEIRQRGGEHVLVNRTMDGRTQEFTRLVFHGG
                     PGSTLEMRLFSEAGLRRELERAGFVDIRVHREPCFEAGIYWHHPWSVPVTAIAG"
     gene            6853..7899
                     /locus_tag="PLW13_09690"
     CDS             6853..7899
                     /locus_tag="PLW13_09690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQY70690.1"
                     /translation="MSDTAAGARGFLLPDLCSATAVLFLLLLSVLLAFTLELFAGGLK
                     AFDWIGLGRASAFILWNMLLSAALLCGLRRRIAGWPLAAGAAASYALVLAVCLASNLA
                     CQSFLGGLRAGGAPTIDPWWLARDLLICALLGGAALRYFHLQGELLRQQRAEMDARIA
                     ALQARIRPHFLFNSMNIIASLIPVDPDGAERAVEDLSALFRASLREGATTVPLADEIE
                     LCERYLRLEQLRLGARLVIERDIGVLPAALRIPPLSVQPLLENAVYHGVQPRPEGGCV
                     RLAVRCEPRMLRIEVSNPLPAAEAGASNGSGMALANIRDRLAAIYGDRAALETGSLEG
                     SFVARLAIPLEGGA"
     gene            7896..8624
                     /locus_tag="PLW13_09695"
     CDS             7896..8624
                     /locus_tag="PLW13_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQY70691.1"
                     /translation="MKLLVVDDEPLARERLKRLLAARGDCAAVAEAANGIEAIAANAT
                     HEPDVILLDIRMPGMDGIEAARHMMAAPHPPAIIFCTAYDEYAIAAFESRAVGYLLKP
                     VQREKLDAALAAARASTRLQLNALATAAREPRRFFSSHTGGATRLIPVADVRVLLADH
                     KYVTACCPGASALLDESLREIEQEFPRQFLRVHRNALVATAHITGIVRRPGGDHALTL
                     AGIELQPQISRRHLAEVRQVIESL"
     gene            8712..9647
                     /gene="hemC"
                     /locus_tag="PLW13_09700"
     CDS             8712..9647
                     /gene="hemC"
                     /locus_tag="PLW13_09700"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQY70692.1"
                     /translation="MTDRILRIATRKSQLALWQAGHVRDRLLAAHPGLRVELLPLSTQ
                     GDRILDVPLAKIGGKGLFVKELETAMLEGRADLAVHSMKDVPMDFPPELGLGAILARE
                     DPHDAFVSNAYPVLEALPEGARVGTSSLRRQCQLRAVRPDLRILDLRGNVNTRLRKLD
                     DGEYDAIVLASAGLLRLGLGERIRARIPATVSLPAVGQGAIGVEIRNDDAGLRALLAP
                     LDDAGCGDCVRAERAMNRAMQGGCQVPIAGFAVIEGANQLWLRGLVGSNDGARIVRAE
                     ARGARTEAERIGAQLAATLLAQGAEAILRELWDAG"
     gene            9652..10467
                     /locus_tag="PLW13_09705"
     CDS             9652..10467
                     /locus_tag="PLW13_09705"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQY70693.1"
                     /translation="MDGALQGIRALVTRPAGQGGALVAAIRAAGGEAHEFPLLEVVAL
                     GVQDAQARATVDDTLGRLGSFDAAIFISTNAVRALLARLADSARTWPAQLQCFAIGSA
                     TAQCLREAGIGCASAAAAMDSEELLAHPGLGPVAGRRIVIFKGVGGRDLLARVLVERG
                     ATVTQCALYRRRLPATASQRELHALIEGRAINVLLLSSAEALANLLGLPGADTAGTIP
                     ADLALVTPGERVAAQARDAGFSAVEPAANATDAAMLAALERLAATLRRRVHTG"
     gene            10464..11570
                     /locus_tag="PLW13_09710"
     CDS             10464..11570
                     /locus_tag="PLW13_09710"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQY70694.1"
                     /translation="MTDAGSKPTFEVLSVETNPPVRRASRLPAALALLLAGAALGVSA
                     WHWFAMREVRAELEEVRASANELAARAGRGPDPRLAEALRGLEQGEARMTGLDLRIDA
                     LGTDTAALRERLAAITVADRRDWALAEAEYLERLASQSLLMGREVRGALALLGAADGI
                     LRDLDDPALHASRAALATDIASLRAVAEFDVEGLYLRLAALVEQVPALALMERSRAEA
                     TGGDAAAEPAGAGSWWSRLTAVLGRYVVVRQRNESIKPLLPLAEEGYLRMNLRLGLEQ
                     AKLALLAAEPAVYRGALHQVQQFAGTYLSPTVVANRAFLDEVTALAAMDVAPALPDIS
                     GSLRALRERPGAAAPAAPAPAPAPAPADATTGGQ"
     gene            11574..>12335
                     /locus_tag="PLW13_09715"
     CDS             11574..>12335
                     /locus_tag="PLW13_09715"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HQY70695.1"
                     /translation="MRRLFVVLLLVLAAAASFAYLVRLDAGYVLIELQGYTVETTAWV
                     AALLVLLGLIAFYYLGRALVLLADLLSRLFTGRPRRHAGFLERWHARRRSHTVRGVLA
                     FAEGRWRVAVKQLARGARHADAPLLNHLLAARASAELGDRELAEGFLQLAREVPGAQQ
                     AVALAQAEAALARGENREALAILDGAGLDPAAQPAGIALQLEALERLGEWARIGTLLP
                     EARRHKVRPEAMLEGVEDRTYRALLSLEGMKPEALR"
BASE COUNT         1682 a         4313 c         4504 g         1836 t
ORIGIN      
        1 gcagcggctg gtgtagttgg agctcatcgc gaaggagtac gcgcccgcgc cggtcacggc
       61 gagcagctcg ccgccggcca gggccaggct gcggttcttg ccgaggaagt cgctggattc
      121 gcacaccggc ccgaccacgt catagacggc gtgctcgatg tcgtggcgcg ggcgcactgc
      181 ctcgatgccc atccaggcgc cgtacagcga gggccggatc aggtcgttca tgccggcatc
      241 gacgatcgca aagcgcttgt cgccgttgtc cttcaggtac tcgacgcggg tgagcagcgc
      301 gccggcgcgc gccacgatcg cgcgcccggg ctcggtgagc agggtgtgcg ggcgattgcc
      361 gaagcgcgcg agcagcgccg cggcgtattc ggcgggcgtg ggcggatgct cctcgccgta
      421 gaccacgccg agcccgccgc ccacgtcgat gtggtcgagc gtgatgccct gcgccgcgag
      481 ctcgtcgacc atcgccagca cgcgctcgag cgcatcgaca aaggggccga gcgcggtcag
      541 ctgcgagccg atgtggcagt cgatgccggt gacgcggatg ttcggcaggg ccgcggcgcg
      601 cgcgtagagc gcgggcgcct cgctgatcga gacgccgaac ttgttctcgc gcaggccggt
      661 ggagatgtac gggtggctct gcgcgtcgac gtccgggttg acgcgaatcg agatcggagc
      721 ggtggtgccg tgcccggcgg cgaccgcctg cagtcgctcg agctcggcgg gcgattcgat
      781 gttgaagcag aggatgcggg cctcgagcgc ggcgacgatg tcctcgtgcg acttggcgac
      841 gccggagaac acgatgcgcc cggggtcgcc cccggccatc agcacgcgcc ggagctcgcc
      901 gccggagacg atgtcgaaac ccgcgccccg gcgcgcgagc aggttcagca ccgcgaggtt
      961 cgagttcgcc ttcacggcgt agcagatcag gtgcgagggc gccgccgcca gcgccgcggc
     1021 gaaggcgtcg tactgcgact cgatcgccgc gcgcgaatag acgtagaccg gtgtgccgaa
     1081 gcgcgcggcg atgtcatcca gcgcgacgtc ctcggcgcac agcacgccgt cacggtagtg
     1141 gaaagcctcc gtcatggcgt gccggcctgg ccgggcggcg tctcgggggg caggaacagc
     1201 gggcccttct ggccgcagcc ggcgagcagc gcgacgccga gcaatgtcga gagaatgagc
     1261 aagcgcacgg tggccggtcc gcaggaaatg gacggcgatt atatagccag cccgtcgcgg
     1321 atggcatcgc tccccgtgag cgcggcgttg atcgaggcct ccagccgctg ccggtggcga
     1381 aggtagtgca cggtctgcag cggcgcgtcg gcgaccggcg ccacggcgct caggtccaga
     1441 tcggtgaggt agaccgggta ggcactcgct tcgtggcgcc gcccgaggat gtacgcgccg
     1501 agtcgcgcgt ggaattcggc gccgagctcg cgctgcgaga aggccacgcc atcgcagtac
     1561 accgtgaaca gcggctcgcc ctcgtgcgcc tcggcgatgg cctgcacctc caggaaggac
     1621 ccgggacggt agcccggggg cgcgggcagc tcctgggcgc tgtagtcccc gccggccgcg
     1681 cggaccaggc ggaaaaagcg cgacggctcg gtcgcgcgcg tcacggcggg atcggcgccg
     1741 ccgctgccgt tctggcgata gcgcaccgat tcgaggaact gcgcgatggc gccgagcagc
     1801 gtgccctcgc gcgcgccggc gtactccgcg cgcaggaagg acccgtgctc gtcgacgatc
     1861 gtgacggcga tggcttcggc ccgcgcctcg ctgaagacgc tgcagcgcca cggcgtcatg
     1921 cgggcggcga tcaccggcaa cggcgtgcgc tccagtgcat ggcggtcgat cgcgagcggg
     1981 gaccactgcg gctgtggctc gcccaggacc tcgtagagcg ccgccaggct gtcggcgctg
     2041 gcactgctgc ccgcgccgcg ccggtgctgc agcacgtgca gctggttgcg gatctccagc
     2101 acgtagcgcg cgctggcgcc acgctcgcgc tggacgtggt gtgcgagatc gccgaacact
     2161 tcccccacgc gggtcgcgat cggctcggcg cggctgggac agaaacagcg caccgcgacg
     2221 cgcaggcccg cattgccgtg cgccagcggc aggaagtcgc gcaggcatcg cagcaggccc
     2281 tccacgccgg catgccgcgc gctgaccagc tcgccccagc tgttgcgtgc cacggtctcg
     2341 acgttgagca cgaggttctc gcgcagcgcg ctgtagccga gggagtcgat gcggctgctg
     2401 acgcgctgca gcccctgttc gcgcgcgagg cgcatcggat ccgcggcgac gttcacgaac
     2461 accagcaggt ccgtgacccg cgcggcgctc gcgaacgcct cgtcgcccgg tgccacgtcg
     2521 gcatgatcgc ccagcacgtg ctcgcgcagt tcacgcagca tgccctgcac ctcgccggcg
     2581 ctcaggccgt cggcgcgctc gcgcaccgcg atgcgcgtgc gctcgccgat cacgccgttc
     2641 acgaggcacc agcacagcag ttcgctcagg ctgggcgcct gcctgagcgc gcgcccggcg
     2701 ggcatggcgc tgccgcgcgt gacgccggcc caggccgccc acagcgccgg cgccccgtcg
     2761 gccacgcgca aggtgccgat gctgatctcg ttctcggtga cgttgatcgc gcgccccggg
     2821 ttcacggcgt ctatcttgcc ggccttgcgc tcgtaggccg cgaagagcct gcgcccgagg
     2881 atgttgaact cgcgcgcctc gatggcgcga gccgcatcgc cgcggcgcgc gaactccgac
     2941 aggaagcggt agctcgaggt gagctccttc accagttcgt tgcgctcggc ctcgacgtcc
     3001 tggatcttcc agtgcgagcg cgagtcgagc agcgcgagct tgtccctgtc ccagccccac
     3061 tcggcgacca ggcgctccat cagcagccgc tgccacgacg gtatgcccgc gcgcgggggc
     3121 tgcgacagct tcttgcccgc cttgtagtac aggcagcggc gcacgatctc caggcgcgcg
     3181 ggctgctcgc gcgccagcag gtgacgctcg atgcgccggt agaccatcac gtaggggtcg
     3241 agctcgtcga tgtcctcgag tccggcgcag accgcgcgct tgtagtcggc gctgaggcag
     3301 gcggcggccg ggtgatcggc cgcatagacc tcgaacagca gcagcttgag taccgatttg
     3361 tagggcgagt cgatggcctt gtagagctgc cagatgccgg cgccgaggaa ctcgcccgcc
     3421 ggcagctccg cgattccccc gaaatccacc agcccggcac ggcctgcgta gcgcttgcgc
     3481 atcaggtggc tgcagtactc gtcgtagtgc tgctcgtgct gcggcgggat cagccaccat
     3541 gccggcaggc gaccggcgag caggatcgcg gtgcggtaga actcgtcgag cagcaggtag
     3601 tgctgcgccg tcccgcagtt ctcgccggtc agcgcctcgc gctggccgcg cttgaatttc
     3661 tcgccgttca tcaggaacag gtgcgcttcc agcccgatgc cgtgcgccca ctgctgcagc
     3721 agcacggcct tgcgcgccag cagcgcgagc tcctcgctgc cgagctcatc ggggtagcag
     3781 atccagatat cgaggtcgct gccgtcggag tgcccgagcg tgcccgagct gcccatcagg
     3841 tagaccgcgt gcaggcgcgg ctcgggcgtg accaggcgct gcgggctgaa gctgatcgcg
     3901 aggcgcctgg cctgcgcgag ttccgtggcc gagggcgtgt agccgctgat gccgcagggg
     3961 gtgtgctgcg agacgtagcc cggcagggcg gggtgatcga tgtgcagcag cagcggcagc
     4021 aggtcgagga actggcgctg gcgcggtgcc agcatgccgc gcacgcgctc gagccgcgcg
     4081 gcgctgatcg cgcgaaagcg cgtgagcagc cgacgcaact ccttgcggtc tatgccttcg
     4141 tcggcaggtg agattgcggc aggcgtgggc atgccgccaa gtatagcggg ccggcgtggc
     4201 ggctcaggac gcggtgcgcg cggcgaggcg cgcgcgcgcc tcggccgccg cctgcgcgac
     4261 ccgccccggc gcggtgccgc cgatgtggtc gcgcgcgctc accgagcctt ccaggctcag
     4321 cacctcgaac acgtccgctc cgatcgtcgc gctgaagccg cgcagcgtct ccagcggcat
     4381 gtcggccagg tcggtgcgcg tggccacgcc cgcggcgacg gccttgccca ccacctcgtg
     4441 cgcgtcgcga aacggcatgc ccttgcgcac caggtagtcg gcgaggtcgg tggcggtcgc
     4501 gaagccgcgg cgcgcggcct cgcgcagggt ggcttcattg acggtcagcg ccggcatcat
     4561 gtcggcgaag gcgtgcagcg agtcgcgcac cgtgtcgatg gtgtcgaaca gcggctcctt
     4621 gtcctcctgg ttgtccttgt tgtatgccag cggttgcgac ttcatcagca tcagcaggct
     4681 catcaggtgg ccggtgacgc gtccgctctt gccgcgcacc agttcgggta cgtccgggtt
     4741 cttcttctgc ggcatgatcg aggagccggt gcagaagcga tcgggcaggt tcacgaagcc
     4801 gaactgcgcc gaggtccaca gcaccagttc ctcgctgaag cgcgagaggt gggtcatcag
     4861 cagcgcggcg aaggcgcaga attcgatcgc gaaatcgcgg tcggcgaccg agtcgaggct
     4921 gttgcgcgtg ggcgcgctga agccgagctc ggccgcggtg aattcgcggt cgatcgggta
     4981 gctggttccg gcgagcgccg cggcaccgag cggcgagacg ttcatgcgcg cacgacagtc
     5041 gagcaggcgc gcgtagtcgc gctccagcat ctcgttccat gccagcatgt ggtgaccgaa
     5101 ggtcacgggc tgggcggtct gcaggtgcgt gaagcccggc atgatggtcg cggcctcgcg
     5161 cgcggcgagc tcgaccagcc cctgctgcag gcgcgtgagc tcggcggcga tggcgtcgat
     5221 ctcgtcgcgc aggtacaggc gcacatcggt ggcgacctgg tcgttgcgcg agcgccccgt
     5281 gtgcagtttc ttgcccgcga tgccgatgcg cgcggtgagg cgcgcctcga tgttcatgtg
     5341 cacgtcctcg agctccacgc tccactcgaa gcggccctcc tcgatctcgc ggcggatctc
     5401 gccgaggccg gcgacgatcg acgcgtgctc ctgtgcgctg agcagcccgg cgcgggcgag
     5461 catcgcggcg tgggccagcg agccgcggat gtcgtgcagc gcgaggcggc ggtcgaagtc
     5521 caccgaggcg gtgaagcgct gcacgaaggc gtcggtgctc tcgctgaaac gcccccccca
     5581 gagggtcgaa gtctgctgct tttccatggt tttccttgat cgcgggctcg agcgggccgc
     5641 gattatagca gcagccccgc agcccgctgc gccgctcgcc gggcgcgcgt caccgatcgg
     5701 gcagcatgcc ggcccgtgcg agtgatacgc aggggattgc gcgcgctcgg ggtacaatcc
     5761 ggaccgtacg gcactgccct cgcgcgcggg tgcccaccca accatcacgt accacggggc
     5821 gcacatgacc acgattcgag ccatgctgga gatggttccc cgcctgagac atcgcatgca
     5881 gcgtctcctg cggccggcgt ccagcctgat cgatacctct gccgccagtc ccgtgcccgc
     5941 cggcacgctg atctaccgct gcaacgtgtg ctcatcgacg tgctcggtcc ctgttgcggc
     6001 gctggagcgc gacggcggga actgcgccgt ctgcgattcg aacgtgcgct ttcgcagcct
     6061 cgtccatctg ctctcgctca ggctgttcgg ccggtcactg gcgatctccg cgtttcccgc
     6121 gcaagccagg cacctgcgcg gcatcggcat gagcgacacc gggcgctacg cggagctgct
     6181 ggcgcaaagg acgggctaca cgaacacctt ctatcacaag gctccgcggc tcgacatcca
     6241 ggcccccgcg tccgggtatc tcgcggccca cgacttcgtg atcagttccg atgtcttcga
     6301 gcacgtcgtg ccgccggtcg cgacggcgtt caccaacctc cgcgcgctgc tcaagcccgg
     6361 cggagtgttg gtgttcacgg tgccattttc gctcgacagc gagacggtgg agcattttcc
     6421 ggagctccac gagttcgaga tacgccagcg cgggggcgag cacgtgctgg tcaaccgcac
     6481 gatggacggg cggacccagg aattcacccg gctggtgttt catggcggac cgggcagcac
     6541 cctcgagatg cggctcttct ccgaggcggg actcaggcgc gagctggaac gggcaggttt
     6601 cgtcgatatc cgcgttcacc gcgagccctg cttcgaggcc ggcatctact ggcatcaccc
     6661 ctggtcggtg ccggttaccg cgattgccgg ctaggcagcc cctgttgcgc cagccgcgcg
     6721 atggctggtg tttgccgggc cggtctgcgg cggcgaggaa cgcgggcgac gaatacgggc
     6781 tagactgtgg cgtgttttca ctgtccgcag cgccatgacc cgcacctcag cccccgcgcg
     6841 gaactcccgc acatgagcga caccgccgcc ggcgcgcgcg gcttcctgct ccccgacctg
     6901 tgctcggcca ccgcggtgct gttcctgctg ctgctcagcg tgctgctggc cttcacgctg
     6961 gagctgttcg cgggcggcct gaaggccttc gactggatcg ggctcgggcg cgcctcggcg
     7021 ttcatcctgt ggaacatgct gctgagcgcg gcgctgctgt gcggcctgcg ccggcgcatc
     7081 gcgggctggc cgctggccgc gggcgccgcg gcgagctacg cgctggtgct ggcggtgtgc
     7141 ctggcttcga acctcgcgtg ccagtccttc ctcggggggt tgcgggcggg tggcgcgccg
     7201 acgatcgatc cctggtggct cgctcgcgac ctgttgatct gcgcgctgct cggcggggcg
     7261 gcgctgcgct atttccacct gcagggcgag ctgctgcgcc agcaacgcgc cgagatggac
     7321 gcgcggatcg ccgcgctgca ggcgcgcatc cgcccgcatt tcctgttcaa cagcatgaac
     7381 atcatcgcca gcctgatccc ggtcgacccc gatggcgccg agcgcgcggt cgaggacctc
     7441 tcggcgctgt tccgcgccag cctgcgcgag ggggcgacca cggtgccgct ggccgacgag
     7501 atcgagctct gcgaacgcta cctgcgcctc gagcagctgc gcctgggcgc gcggctcgtc
     7561 atcgagcgcg acatcggcgt gctgccggcg gcgttgcgca tcccgccgct cagcgtgcag
     7621 cccttgctgg agaacgcggt gtaccacggt gtgcagccgc ggcccgaggg cggttgcgtg
     7681 cgcctggccg tgcgctgcga gccgcgcatg ctgcgcatcg aggtgagcaa cccgctgccc
     7741 gcggcggagg cgggcgcgag caacggcagc ggcatggcgc tggcgaacat ccgcgaccgg
     7801 ctggcggcga tctacggcga tcgcgcggcg ctcgaaaccg ggagcctcga gggcagcttc
     7861 gtcgcgcgcc tggcgatccc gctggagggc ggcgcatgaa actgctggtc gtcgacgacg
     7921 agccgctggc gcgcgagcgc ctgaagcggc tgctcgccgc ccgcggggac tgcgcggccg
     7981 tggccgaggc cgccaacggc atcgaggcca tcgcggcgaa cgccacgcac gagcccgatg
     8041 tcatcctgct cgacatccgc atgcccggca tggacggcat cgaggcggcg cgccacatga
     8101 tggccgcgcc gcatccgccc gcgatcatct tctgtaccgc ctacgacgag tacgcgatcg
     8161 cggctttcga gagccgcgcc gtgggctacc tgctcaagcc ggtgcagcgc gagaagctcg
     8221 acgccgcgct cgccgcggca cgcgcgagca cgcgcctgca gctgaacgcg ctggcaaccg
     8281 cggcgcgcga gccgcgccgc ttcttcagca gccacaccgg cggcgcaacg cgcctcattc
     8341 cggttgcgga tgtgcgcgtg ctgctggcgg accacaagta cgtcaccgcc tgctgtcccg
     8401 gcgccagcgc gctgctcgac gagagcctgc gcgagatcga gcaggaattc ccgcgccagt
     8461 tcctgcgggt gcaccgcaac gcgctggtcg ccaccgctca catcaccggc atcgtgcgtc
     8521 gccccggcgg tgatcacgcc ctgacgctcg cgggcatcga gctgcaaccc cagatcagcc
     8581 ggcgccacct ggccgaggtg cgccaggtca tagaaagtct ttgaaacccg tccgcaacag
     8641 caagccgccg gcacgccatt cgcgccgggc tgccgtatca tgtcgccctc acttccgtcg
     8701 cagttcctga catgaccgac cgcatcctcc gcatcgcaac gcgcaagagc cagctggccc
     8761 tgtggcaggc cgggcacgtg cgcgatcgcc tgctcgccgc gcacccggga ttgcgcgtcg
     8821 agctgctccc gctatcgacg cagggcgatc gcatcctcga cgtgccgctg gcgaagatcg
     8881 gcggcaaggg cctgttcgtg aaggagctcg agaccgcgat gctcgagggg cgcgcggacc
     8941 tcgcggtgca ctcgatgaag gacgtgccga tggatttccc gcccgagctg gggctgggcg
     9001 cgatccttgc gcgcgaggac ccgcacgacg ccttcgtttc caatgcgtac ccggtgctgg
     9061 aagcgctgcc cgagggagcg cgcgtgggta cgtccagcct gcgccgccag tgccagctgc
     9121 gcgcggtgcg gccggacctg cgcatcctcg acctgcgcgg caacgtgaac acgcggctgc
     9181 gcaagctcga tgacggcgag tacgacgcca tcgtgctcgc gagcgcggga ctgctgcgcc
     9241 tggggttggg tgagcgcatc cgcgcgcgca ttcccgccac ggtttcgctg cccgcggtgg
     9301 ggcagggggc catcggcgtc gagatccgca atgacgacgc cggcttgcgc gcgctgctgg
     9361 cgccgctgga cgacgccggc tgcggtgact gcgtgcgcgc cgagcgcgcg atgaaccgcg
     9421 cgatgcaggg cggctgccag gtgcccatcg cgggctttgc ggtcatcgag ggcgcgaacc
     9481 agctgtggct gcgcgggctg gtcggcagca acgacggcgc gcgcatcgtg cgcgccgagg
     9541 cgcgcggtgc gcgcaccgag gccgaacgca tcggtgcgca gctcgcggcc acgctgctcg
     9601 cgcagggcgc cgaggcgatc ctgcgcgagc tctgggacgc gggctgatca cgtggatggc
     9661 gccctgcagg gcatccgcgc gctggtgacg cgcccggcgg ggcagggcgg tgcgctggtc
     9721 gcggccatcc gcgcggccgg cggcgaagcg cacgaatttc cgctgctcga ggtggtggcg
     9781 ctgggcgtgc aggatgcgca ggcccgcgcg acggtcgacg ataccctggg ccggctcggt
     9841 tcgttcgatg ccgcgatctt catcagcacc aatgccgtgc gcgcgctgct ggcgcgcctt
     9901 gccgacagcg cgcgcacatg gccggcgcaa ctgcaatgct tcgcgatcgg cagcgccacg
     9961 gcgcagtgcc tgcgcgaggc cggaatcgga tgtgcgagcg cggccgcggc gatggacagc
    10021 gaggaactgc tcgcgcaccc cgggctcggt ccggtcgcgg gccggcgcat cgtgatcttc
    10081 aagggcgtcg gcgggcgcga tttgctggcc cgggtgctcg tggagcgcgg cgcgacggtg
    10141 acgcagtgcg cgctgtaccg tcgtcgcctg cccgccactg catcgcagcg ggaactgcat
    10201 gcgctgatcg aggggcgcgc catcaacgtg ctgctgctga gcagcgccga ggcgctggca
    10261 aacctgctcg gcttgcccgg tgcggacacg gctggcacga tacctgcgga cctggcgctg
    10321 gtcacaccgg gtgaacgcgt cgcggcacag gcgcgcgatg ccggtttcag cgctgtcgag
    10381 cctgcagcca acgccaccga cgcggcaatg ctcgcggcgc tggaacggct ggcagccacc
    10441 ctacgcagac gagtgcacac aggatgacag acgcaggcag caagcccaca ttcgaggtgc
    10501 tctcggtgga aaccaacccg ccggtacgcc gcgcgtcccg gctccctgcc gcgctggcgc
    10561 tgctgctggc gggcgccgcg ctcggcgtga gcgcttggca ctggttcgcg atgcgcgaag
    10621 tccgtgccga gctcgaggag gtgcgcgcga gcgccaacga gctcgcggcg cgagccgggc
    10681 gcggccccga tccccgcctg gccgaggcct tgcgcggcct ggagcagggc gaggcgcgca
    10741 tgaccgggct cgatttgcgc atcgacgcgc tgggcaccga caccgccgcg ctgcgcgagc
    10801 ggctcgccgc gatcacggtg gccgatcgtc gcgactgggc gctggccgag gccgaatacc
    10861 tggagcggct ggcgagccag agcctgctga tgggccgcga ggtgcgcggt gcgctggcgc
    10921 tgctcggcgc cgccgacggc atcctgcgcg acctcgacga tcccgcgttg cacgcctcgc
    10981 gcgccgcgct cgccactgac atcgccagcc tgcgcgcggt ggcggaattc gatgtcgaag
    11041 gcctgtacct gcgcctcgcc gccctggtgg agcaggtgcc cgcgctcgcg ttgatggagc
    11101 gttcgcgcgc cgaggccacc ggcggagatg ccgctgccga acccgcgggc gccgggagct
    11161 ggtggtcgcg cctcaccgcc gtgctcggtc gctatgtcgt ggtgcgccag cgcaacgaat
    11221 cgatcaagcc gttgctgccg ctcgccgagg aggggtacct gcgcatgaac ctgcgtctgg
    11281 ggctggagca ggcgaagctc gcgctgctcg cggcggagcc cgcggtctac cgcggcgcgc
    11341 tgcaccaggt gcagcagttc gccggcacct acctgagccc gacggtcgtg gccaaccggg
    11401 cttttctcga cgaagtgacg gccctcgccg cgatggacgt ggcgcctgcg ctgcccgata
    11461 tctcgggttc gctgcgtgcg ctgcgcgagc ggccgggtgc ggcggcgcca gcagcgccgg
    11521 cacctgctcc cgcaccggcg cccgccgacg cgaccaccgg cgggcaatag accatgcgcc
    11581 gcctcttcgt cgtcctgctg ctggttctcg ccgccgcggc gagcttcgcc tatctcgtgc
    11641 gcctcgatgc cggctacgtg ctgatcgagt tgcagggcta caccgtcgag accacggcgt
    11701 gggtggcggc gctgctggtg ctgctcgggc tgatcgcgtt ctattacctg ggccgcgcgc
    11761 tggtgctgct ggccgacctg ctgagccgcc tgttcaccgg acgcccgcgc cggcatgcgg
    11821 gtttcctgga acgctggcac gcgcggcggc gcagccacac cgtgcgcggc gtgctggcct
    11881 tcgccgaggg gcgctggcgc gtggccgtca agcagctcgc gcgcggcgcg cgccatgccg
    11941 atgcaccgct gctgaaccac ctgctggcag cgcgggcgag cgcggaactg ggcgaccggg
    12001 aactggccga gggcttcctg caactcgcca gggaagtgcc cggcgcgcaa caggcggtgg
    12061 ctctggcgca ggccgaggcc gcgctcgcgc gtggcgagaa cagggaggcg ctcgcgatac
    12121 tggacggcgc gggcctcgat ccggcggcgc agccggccgg catcgcgctg cagctcgagg
    12181 cgctcgagcg cctgggtgag tgggcgcgca tcggcacgct gctgcccgag gcgcgccgcc
    12241 acaaggtgcg tcccgaggcg atgctggagg gtgtcgagga ccgcacctac cgcgcactgc
    12301 tgtcgctcga gggcatgaag cccgaggcgc tgcgc
//
LOCUS       DAORUQ010000098        12325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_5179_length_12325_cov_5.667971, whole genome shotgun sequence.
ACCESSION   DAORUQ010000098 DAORUQ010000000
VERSION     DAORUQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_12325_cov_5.667971"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLW13_09720"
     CDS             complement(<1..554)
                     /locus_tag="PLW13_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQY70696.1"
                     /translation="MLVDLHTHTSASDGELAPLALLQMQAGAGVGLMAFTDHDTLDGW
                     DRIAAMETTAAGLPRLLPGIEFSVDAAGREIHVVGLGFDPAHPAIRAGIAAQQLRRRA
                     RAERIAARLEYLGVHGALAGAEHEAAGAGLGRPHFARFLVASGRVRTLDEAFHRYLGK
                     GKPAACRMEWPALDEATGWIRAAGG"
     gene            670..1266
                     /locus_tag="PLW13_09725"
     CDS             670..1266
                     /locus_tag="PLW13_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HQY70697.1"
                     /translation="MKQLAELLPVVLFFVVYQLDGRSLALGGWEYHFDGIYSATAVLM
                     IATVLQLALTRLLSGHIEKRLLLLTAVVCAFGAATLLLRNQLFIQWKPTIFNWVLAAV
                     FVGTHTLTGKTVMERAIGAQLSLPAVAWRRLNSLWVANFVVVGALNLFVAYRFSEAAW
                     VSYKLYSAIGFTLLVTALTVALIMPHVKDGEEPPAGAG"
     gene            1293..1592
                     /locus_tag="PLW13_09730"
     CDS             1293..1592
                     /locus_tag="PLW13_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQY70698.1"
                     /translation="MYYAIIAEDAENSLERRLATRPDHLARLTRLRDEGRLLLAGPHP
                     ALDCEDPGAAGFTGSLVVAHFDDLAAAQAWAADDPYVHAGVYARVTVKPFRRVLP"
     gene            1697..2350
                     /locus_tag="PLW13_09735"
     CDS             1697..2350
                     /locus_tag="PLW13_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HQY70699.1"
                     /translation="MFSRLVGALLLSSAVIMPVAAQEQRYVEDTRAVPLREGAALDQR
                     TLLDVPTGTPVTVVQEQRDTGWALVRTREGAEGWMPLRFIKREPGARYQVVNALRLLG
                     QPDDGSVTLSAAVEQGRKELADMAAARDALQAELTELKQLSSNATQLDASNRDLNEQL
                     HVLKNRIGVLEADNQRLRDDSWQKWFINGVWATGIGGLLTLLLPRLIPQRKRRSEWV"
     gene            2379..2963
                     /locus_tag="PLW13_09740"
     CDS             2379..2963
                     /locus_tag="PLW13_09740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY70700.1"
                     /translation="MITRLLAVLALLGGALLFVATPAQTDADANPRVRIGTNAGDITI
                     ELYPDEAPLTVANFLSYVDSGFYAGTVFHRVIPNFMIQGGGFTQELAEKPTGEPVKNE
                     SRNHLHNERGTIAMARTDDPDSAGAQFFINVRANLTLDFNYVTRKPGYTVFGRVIDGM
                     DVVDGISLVDTQRAGHLDDVPVNPIVIESITRLE"
     gene            2960..3733
                     /locus_tag="PLW13_09745"
     CDS             2960..3733
                     /locus_tag="PLW13_09745"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQY70701.1"
                     /translation="MSICLSVNLNKIALIRNSRAGDYPSVTEHARLCIEAGADGITVH
                     PRPDQRHIRAADVPALAAMLAVELNVEGNPFAGPARSHRADVADYPGFMTLVRAVRPA
                     QCTLVPDSDAQLTSDHGFDLAREGERLRPLIEELRALGIRVSLFMDPDPAQIRRAATC
                     GADRVELYTGPYAAAFAAGADPQAILRPHVEAARAALDAGLGVNAGHDLNLLNLGAYA
                     AAVPGLQEVSIGHAFTVDALRLGIDATVRAYQRCLGQAV"
     gene            3730..5154
                     /locus_tag="PLW13_09750"
     CDS             3730..5154
                     /locus_tag="PLW13_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQY70702.1"
                     /translation="MNDAFAEGVSGEFSVCDCDRYAEHLADISVRRRMVIASEDVRNQ
                     FGVLLLARGAPLGRFAFGRMHGHRLNRPIDEALAMSRCLDNGALASRLRELLDCAPDL
                     QRIAVATGLAPRLEALCETAALGPALLQKLSVLEEVLPAVFHRALFCACFGVLLAGER
                     RLDATEARLLFQAGLLHDLGLLHIDPALACGNAGIGPGEWDEIRRHVPIGATIAARLA
                     TAPARLAAVIRGHHERADGTGYPAGSTGEDIDPLAQMLALCDMLHALRFHGPDAGTLA
                     DCMPYLRVNRHLFGEQNYRAVGRLLAGARGLAAPAAPRSLPRQLLLDTNRSLVVLRGS
                     FATLRALLRQFPADPLPASVQRLVEQFERISDASGLGLEACAAALGESIGADTGEAGL
                     IEIQRTAQELFWLVRRIERQLRALAGATAAGASQRMLRELAHGVEFELGRAWQRFEGA
                     SAPRLQRGNDALTPLPLRASLPRR"
     gene            5176..6036
                     /locus_tag="PLW13_09755"
     CDS             5176..6036
                     /locus_tag="PLW13_09755"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HQY70703.1"
                     /translation="MRADDEERITEPRATGRGLARPALFVLLALALVAVTTLGVRELL
                     RATPTPGAIPAAPAPPGAASAPAPPAATTPGAAQAAPAEPMPPAEPAAPPLPALAQSD
                     VEVRTTLADIVPAPAHPSLAPDNLLARAAALADGFGRGKLVRDKLPLPAIAGKLIVVE
                     RGERIFLDTANYARYDAVVAAFAELDTAALARWYLRYEPLLQQAYEELGNGEERVREA
                     ILDGIELMLAAPAPAGELELKQPAVFYKYADPALEALPESQKLLIRMGPRNRAIIVAQ
                     LQRLRQALGS"
     gene            complement(6031..7407)
                     /locus_tag="PLW13_09760"
     CDS             complement(6031..7407)
                     /locus_tag="PLW13_09760"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HQY70704.1"
                     /translation="MTERDKTTLKRIRTGSFERRLSLTRSGLLAGTRYMAQSASSLLL
                     PRARRAEHRRAALGAQARYLVEELGKLKGSVVKIGQMMALYGEHFLPDEVTVALHVLE
                     DRTVALEWPVIRRVLERELGRQRLAELDIDPVPIGAASLGQVHLARRLADDRALCLKV
                     QYPGVADSIDADIDAVVRMLELGRMVAPTEEFRSWLEEVREMLHREVDYTLEAAKTQL
                     FHERLKRDPRFVVPAVIPEFSTAHVLATSFEQGYAVTSADAEALPLARRNALGRAFLE
                     LFLREIFEWKELQTDPNFGNYRVRPAATARGRDRLVLLDFGAVQQYPDSFLDPLKQMI
                     RGAYQGDIEQVRQGALELRFIRPEFPAHVQQSFAEVCAGVIEPLVCRPGNVPAQALNR
                     EGQYRWRDSDLPSRIASRAAKAAFSTYFRVPPREFVFLNRKLIGVYTFIAVLGAEFNG
                     ADILEKYL"
     gene            7494..8579
                     /locus_tag="PLW13_09765"
     CDS             7494..8579
                     /locus_tag="PLW13_09765"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQY70705.1"
                     /translation="MSNDDLLIRVALLLAVSLAAIALAWRARQYNRRLAPVTRGTGIA
                     SAGAAPAPAIPPGTPFRDPMADAMLRELNRCAFGVARFAHALPDEHETMLETLRRAAL
                     APVAEPPALLVPLLAAGGDDAALHGLLDTLRRDPPLAGAVLELAADPAFGHCGEPFTG
                     LEHALEALGAAPLRALIASALLQPAFALPAQPFAAFGRAAWQLGLQSALAAAAFARAG
                     ASGDPVAAHVLGLVHALGAVTVFRTALAHYQATPGLAPRAEVFVRLVTERADAAAHAL
                     AVAWNLPAGMRSALAEHSAQLAPGAMSAPGRALYAGRLFGLAAVLREHDALDHAGFGE
                     LLARKGLDDAHRQWRPSTPGAARQAAR"
     gene            8576..9325
                     /gene="cmoA"
                     /locus_tag="PLW13_09770"
     CDS             8576..9325
                     /gene="cmoA"
                     /locus_tag="PLW13_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HQY70706.1"
                     /translation="MSTKHTPRHGARDAVYAEAREQIAAFRFDAAVAEVFADMIARSV
                     PGYALTLQLIAVAARRFAQPGSRCYDLGCSLGASTLAIRQNAPAACHVIGIDNSADMV
                     ERCRAIVATDPSPVPVEIRCEDVCETALGDASLVTLNFTLQFIPPAGRAALLARIATG
                     MRPGGCLVLSEKLAFDDPAEQELLQTLHHDFKQLQGYSALEVAQKRAAIENVLIPETL
                     ATHRRRLLAAGFRRVTLGLQCLNFASLLAEK"
     gene            9322..10296
                     /gene="cmoB"
                     /locus_tag="PLW13_09775"
     CDS             9322..10296
                     /gene="cmoB"
                     /locus_tag="PLW13_09775"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HQY70707.1"
                     /translation="MSLLAGLGDALATAPLGGAVAPLGEALQRCLAQLRHGDAGDWEA
                     ALAALPALRPTDVDLGNAAVRIGSAAQIDPEARARLHEALQQLHPWRKGPFSLFGIDI
                     DTEWRSDLKWARIAPHLDLGGRRVLDVGCGNGYYALRMLGAGAGYVLGIDPTLRFLAQ
                     FRALQHYLDDPRALLLPLRAEDLPPRLGCFDTVFSMGVLYHRRSPFEHLGELLDALRP
                     GGELVLETLVVDGDAERVLVPADRYAMMRNVWFIPSPAAMLGWLRRAGFRDVRLVDVA
                     ATTTQEQRRTPWMRFESLADFLDPADPRLTREGYPAPLRAVFMATRAR"
     gene            10469..11518
                     /locus_tag="PLW13_09780"
     CDS             10469..11518
                     /locus_tag="PLW13_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY70708.1"
                     /translation="MNQPATPRKPHIIVADTMPERYARGWHVLDKASEFTTKPRMFDV
                     FGTRLAVYRGDKDGQVHVLDAYCPHMGADLSKGYVKDDSLVCPFHAWSWGGDGVCNDI
                     PYAKKIPDKAVIKSWPVLEKNGLLFVWNDPENNPPIAAQEPARMEDYYSGAWTDWTMT
                     QVPIENNCRELVDNMADLGHFGPVHYSRVKSFKNIQDGHRYIQVMEGGHEILEDGTTG
                     MTTTAIYEGPAYMTTTMTGSLYGRPVTVHLLVSHYPVHMNKFLINFGVMMKKDPNLPD
                     VVNRDMVDKYTRMSIDSFIQDVEIWDNKVRVGNPVLCDGDGPIHMLRKWYSQFYLNVA
                     DIPEDLVKHKEHVSL"
     gene            11550..>12325
                     /locus_tag="PLW13_09785"
     CDS             11550..>12325
                     /locus_tag="PLW13_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY70709.1"
                     /translation="MIDTKALGYVVIETTDLAKWEHFGTRVMGLMVAPSVQEDGVLYL
                     KMDEYPYRFRIVQGEQDRFGAAGWEVAGPQEFAQAVRELEAAGIACERAPAGACKARL
                     LSEMVAFTAPGGLRCELFYYLQLDYLPLVSPVGVKAFVTGFNGTMGLGHIAAATPQLE
                     ASHRFFTEVMGFGQTDYLHLHFSPDPADPGQGLHFLHASNPRHHSLALFQSKDPLPGD
                     LVHLMVEVPDLDTLGGFMDRVHSNGVRVITCLGRHTNDRMV"
BASE COUNT         1862 a         4520 c         4162 g         1781 t
ORIGIN      
        1 ccgccggcgg cgcggatcca gcccgtcgcc tcgtccaggg cgggccattc catgcggcag
       61 gccgccggct tgcccttgcc gaggtagcgg tggaaggcct cgtccagggt acgcacccgc
      121 ccgctcgcca ccaggaaacg cgcgaaatgc ggccgcccca ggccggcgcc ggccgcctcg
      181 tgctcggctc ccgccagcgc gccgtgcacg cccaggtact cgagccgtgc cgcgatccgc
      241 tcggcgcgcg cgcgccggcg cagctgctgc gcggcaatgc cggcgcggat cgccggatgc
      301 gccgggtcga agcccagccc caccacgtgg atctcgcgcc ccgcggcatc caccgagaat
      361 tcgatgccgg gcagcaggcg cggcagcccg gcggcagtcg tctccatggc ggcgatgcgg
      421 tcccacccgt ccagcgtgtc atggtcggtg aaggccatga gccccacgcc ggcgcccgcc
      481 tgcatctgca gcagcgcgag cggcgccagc tcgccgtcgg aggcgctggt gtgggtgtgc
      541 aaatcgacga gcacggaaaa agcctgtgtg gaaacggcgg cggggacgat agaatagcgg
      601 cattcgcggc ccggtgccgc ggcaaattcc cgcagcgcgc cgcgccgcgc ccgatacggc
      661 cccgatccca tgaagcaatt ggcggaactc ctccctgtcg tgctgttctt cgtcgtctac
      721 cagctggacg ggcggtcgct ggcgctgggg ggctgggaat accacttcga cggcatctac
      781 tcggccaccg ccgtgctgat gatcgccacg gtgctgcagc tggcactgac acggctgctg
      841 agcgggcaca tcgagaagcg cctgctgttg ctcaccgcgg tggtctgcgc cttcggcgcc
      901 gccacgctgc tgctgcgcaa ccagctgttc atccagtgga agcccacgat cttcaactgg
      961 gtgctcgcgg ccgtcttcgt cggcacccac accctgaccg gcaagaccgt gatggaacgg
     1021 gcgatcggcg cccagctgag cctgcccgcc gtcgcctggc gacgactgaa cagcctctgg
     1081 gtggcgaatt tcgtggtcgt cggcgcgctg aacctcttcg tggcctaccg cttcagcgaa
     1141 gccgcgtggg tcagctacaa gctctacagc gccatcggct tcacgctgct ggtgaccgcg
     1201 ctgaccgtgg cgctgatcat gccccacgtc aaggacggcg aggaaccgcc ggccggcgcc
     1261 ggctgacaca cccgcaacgg agcaaacgca tgctgtatta cgcgatcatc gccgaagacg
     1321 ccgagaactc cctggagcgg cggctcgcga cccgtcccga ccacctggca cggctcacgc
     1381 gcctgcgcga cgagggccgg ctgctgctcg ccgggccgca cccggcgctc gactgcgagg
     1441 atccgggtgc cgcgggcttc accggcagcc tggtcgtggc gcacttcgac gacctcgccg
     1501 cggcccaggc atgggccgcg gacgatccct acgtgcatgc cggcgtctat gcccgcgtga
     1561 ccgtcaagcc gtttcgcagg gtgctgccct gagccgcccg cgccggcttc cccgccggcg
     1621 catttgacat cggattaatc gaaactagat taatgggtta gtgcagtttt tgaatccatc
     1681 atcagagctt ttcgccatgt tttccagact cgtcggcgcg ctgctgctca gcagcgccgt
     1741 gatcatgccc gttgcggcgc aggagcagcg ctatgtcgag gacacccgcg cggtgccgtt
     1801 gcgcgagggc gccgccctgg accagaggac cctgctcgac gtgccgaccg gcactcccgt
     1861 gaccgtggtg caggagcagc gcgacaccgg ctgggcgctg gtgcgcaccc gcgagggcgc
     1921 cgagggctgg atgccgctgc gcttcatcaa gcgcgagccc ggggcgcgct accaggtggt
     1981 caacgcgctg cgcctgctcg ggcagcccga cgacggcagc gtgacgctgt cggcggcagt
     2041 agagcagggg cgcaaggaac tcgccgacat ggccgccgcg cgcgacgcgc tgcaggccga
     2101 gctcacggaa ctcaagcagc tctccagcaa cgccacgcag ctcgacgcca gcaaccgcga
     2161 cctgaacgag cagctgcacg tgctgaagaa ccgcatcggg gtgctcgagg ccgacaacca
     2221 gcgcctgcgc gacgactcct ggcagaaatg gttcatcaac ggcgtctggg cgaccggcat
     2281 cggcgggctg ctcacgctgc tgctgccgcg cctgatcccg cagcgcaagc gccgctcgga
     2341 atgggtctga gccacaacca cacgggacca atgacaccat gatcacccga ctgctcgccg
     2401 tgctcgccct gctgggcggc gccctgttgt tcgtcgcgac accggcacag accgacgccg
     2461 acgccaatcc acgggtgcgc atcggcacca atgccggtga catcaccatc gagctctatc
     2521 cggacgaagc gccactgacc gtggcgaact tcctgtcgta cgtcgacagc ggcttctacg
     2581 ccggcaccgt gttccaccgc gtgatcccca acttcatgat ccagggcggg ggcttcacgc
     2641 aggagctcgc cgagaagccg accggcgagc cggtgaagaa cgaatcgcgc aatcacctgc
     2701 acaacgagcg cggcaccatc gcgatggcac gcaccgacga ccccgacagc gcgggggcgc
     2761 agttcttcat caacgtgcgc gcgaacctga cgctcgactt caactacgtc acgcgcaagc
     2821 ccggctacac ggtattcggg cgcgtgatcg acggcatgga cgtcgtcgac ggcatctcgc
     2881 tggtcgatac gcagcgcgcc gggcacctcg acgacgtgcc ggtgaacccg atcgtgatcg
     2941 agtcgatcac gcgactcgaa tgagcatctg cctctcggtc aacctgaaca agatcgcgct
     3001 gatccgcaat tcgcgcgcgg gcgactatcc cagcgtgacg gagcacgcgc gcctgtgcat
     3061 cgaggccggc gccgacggca tcaccgtgca tccgcgcccc gaccagcgcc acatacgcgc
     3121 cgccgacgtg cccgcgctgg cggcaatgct ggcggtggaa ctcaacgtcg agggcaaccc
     3181 cttcgccggc cccgcgcgca gccaccgcgc cgacgtcgcc gactatcccg gtttcatgac
     3241 gctggtgcgc gcggtgcgcc cggcgcagtg cacgctggtg cccgacagcg acgcgcagct
     3301 cacctcggac cacggcttcg acctcgcgcg cgaaggcgaa cgcctgcgcc cgctgatcga
     3361 ggagctgcgc gcgctgggca tccgcgtcag cctgttcatg gatcccgatc ccgcgcagat
     3421 ccgccgcgcc gccacctgcg gcgcggaccg cgtcgagctc tacaccgggc cctacgcggc
     3481 ggcctttgcg gccggcgccg acccgcaggc gatcctgcgc ccgcatgtcg aggctgcgcg
     3541 cgccgcgctc gatgccggcc tcggcgtgaa tgccggccac gacctgaacc tgctgaacct
     3601 cggcgcctac gcagcggccg tgccggggct gcaggaagtc tccatcggcc acgccttcac
     3661 cgtcgatgcc ctgcgcctgg gcatcgacgc gaccgtgcgc gcctaccagc gctgcctggg
     3721 acaagccgtt tgaacgacgc cttcgccgag ggggtcagcg gcgaattctc ggtctgcgac
     3781 tgcgaccgct acgccgagca cctcgcggac atcagcgtgc gacgacgcat ggtcatcgcc
     3841 agcgaggacg tgcgcaacca gttcggcgtg ctgctgctcg cgcgcggcgc gccgctcggg
     3901 cgcttcgcct tcggcaggat gcacggccat cgcctgaacc gccccatcga cgaagcgctc
     3961 gcgatgagcc gctgcctcga taacggcgca ttggcctcgc gcctgcgcga actgctggac
     4021 tgcgcgcccg acctgcagcg catcgccgtg gccaccggcc tcgccccgcg cctggaggcg
     4081 ctgtgcgaga cggccgcgct gggccccgcc ctgctgcaga aactcagtgt gctcgaggaa
     4141 gtcctgccgg ccgtgttcca ccgcgcgctg ttctgcgcct gctttggcgt gctgctggcc
     4201 ggggagcggc gcctcgatgc caccgaggcc aggctgctgt tccaggccgg cctgttgcac
     4261 gacctcgggc tgctgcatat cgatccggcg ctcgcctgcg gcaacgccgg catcggcccc
     4321 ggcgaatggg acgagatccg gcgccacgtg cccatagggg caaccatcgc cgcgcgcctc
     4381 gccacggcgc cggcgcggct ggcggcggtg atacgcgggc accatgaacg cgccgacggc
     4441 accggctatc cggcaggcag caccggtgag gacatcgatc cgctggcgca gatgctcgcg
     4501 ctctgcgaca tgctccacgc gctgcgcttc cacggtcccg acgccgggac gctggccgac
     4561 tgcatgcctt atctgcgggt caaccggcac ctcttcggcg agcagaacta ccgcgcggtg
     4621 gggcgcctcc tcgccggcgc gcgcggtctc gcggcgccgg ccgcgccacg cagcctgccg
     4681 cggcaactgc tgctcgacac caaccggtcg ctggtcgtgc tgcgcggcag cttcgcgacg
     4741 ctgcgcgcgt tgctgcgcca gttcccggcc gatccgctgc ccgccagcgt gcagcggctg
     4801 gtggaacagt tcgagcggat cagcgacgcc tcgggcctgg gcctcgaagc ctgcgcggcc
     4861 gcgctgggcg agagcatcgg cgcagacacc ggcgaggccg ggctgatcga gatccagcgc
     4921 accgcgcagg agctgttctg gctggtgcgc cgcatcgagc gccagctgcg cgcgctggcc
     4981 ggggccaccg ccgcgggcgc ctcgcagcgc atgctgcgcg aactggcgca cggcgtggaa
     5041 ttcgagctcg gccgcgcctg gcagcgcttc gagggagcga gcgcgccgcg cctgcaacgc
     5101 ggcaatgacg cattgacccc cctgcccctg cgcgctagcc tgccgcgccg atgaacaccc
     5161 cgggaggcgc cgcccatgcg cgcggacgac gaggaacgaa tcactgagcc gcgcgcgacg
     5221 ggccgcggcc tggcacgccc ggcgctgttc gtgctgctgg cactggcgct ggtggcggtg
     5281 acgacgctgg gcgtgcgcga gctgctgcgc gccacgccga cgccgggcgc gatcccggcc
     5341 gcgcccgcgc cccccggcgc ggcctccgca cccgcgcccc ctgccgccac gacacccggc
     5401 gcggcgcagg ccgcgcccgc cgaaccgatg ccccctgccg agcccgcggc gccaccgctg
     5461 ccggcgctgg cgcagagcga cgtcgaggtg cgcaccaccc tggccgacat cgttcccgcg
     5521 ccggcgcatc ccagcctcgc accggacaac ctgctcgcgc gtgccgccgc gctcgccgac
     5581 ggtttcgggc gcggcaagct ggtgcgcgac aagctgccgt tgcccgcgat tgcgggcaag
     5641 ctgatcgtgg tggaacgcgg cgagcggata ttcctcgata ccgcgaacta cgcgcgttac
     5701 gacgccgtgg tcgcggcctt cgcggaactg gacaccgcgg ctctggcgcg ctggtacctg
     5761 cgctacgaac cactgctgca gcaggcgtac gaggaactcg gcaacggcga ggagcgcgtg
     5821 cgcgaggcga tcctcgacgg catcgagctg atgctggccg cgcccgcccc cgcgggcgag
     5881 ctggaactga agcagccggc agtgttctac aagtacgccg acccggcgct ggaggcgctg
     5941 ccggagtcgc agaagctgct gatccgcatg gggccgcgca atcgcgcgat catcgtcgcg
     6001 cagctccagc ggctgcgcca ggcgctgggc tcataggtac ttctcgagga tgtcggcgcc
     6061 gttgaactcg gcccccagca ccgcgatgaa ggtgtagacc ccgatcagct tgcggttgag
     6121 gaacacgaac tcgcgtggcg gcacgcggaa ataggtgctg aaggccgcct tcgcggcgcg
     6181 cgaggcgatg cgcgagggca gatcgctgtc gcgccagcgg tactggccct cgcggttcag
     6241 cgcctgcgcc ggcacgttgc ccgggcggca caccagcggc tcgatcacgc cggcgcagac
     6301 ctcggcgaag gattgctgca cgtgtgcggg gaattccggg cggatgaacc tgagctccag
     6361 cgcgccctgg cgcacttgct cgatgtcacc ctggtacgcg ccgcggatca tctgcttcag
     6421 cggatcgaga aaactgtcgg ggtactgctg cacggcgccg aaatcgagca ataccaggcg
     6481 gtcgcgcccg cgcgccgtcg cggcgggccg cacgcgatag ttgccgaagt tcggatcggt
     6541 ctgcagttcc ttccactcga atatctcgcg caggaacaac tcgaggaacg cgcgccccag
     6601 cgcgttgcgc cgcgccagcg gcagtgcctc ggcatcggcg ctggtcaccg cgtagccctg
     6661 ctcgaagctg gtggccagca cgtgcgcggt tgagaactcc ggtatcaccg cgggcaccac
     6721 gaagcgcggg tcgcgcttca gccgctcgtg gaacagctgc gtcttcgcgg cctcgagcgt
     6781 gtaatcgacc tcgcggtgca gcatctcgcg cacctcctcg agccaggaac ggaactcctc
     6841 ggtgggcgcc accatgcgtc cgagctcgag catccgcacc accgcgtcga tatcggcgtc
     6901 gatcgagtcg gccacgcccg gatactgcac cttcagacac agcgcgcgat cgtccgcgag
     6961 ccggcgcgcc aggtgtacct gccccagcga ggcggcgcct atggggaccg ggtcgatgtc
     7021 gagctccgcc agccgctggc gcccgagctc gcgctcgagc acgcgccgga tcacgggcca
     7081 ctccagcgcg acggtgcgat cctccagcac atgcagcgcg accgtgactt catcgggcag
     7141 gaaatgctcg ccgtagagcg ccatcatctg cccgatcttg accacgctgc ccttgagctt
     7201 gccgagctcc tcgacgaggt agcgggcctg cgcgccgagc gcggcacgac ggtgctcggc
     7261 gcgccgcgcc cgcggcagca gcagcgagga ggcggactgc gccatgtagc gcgtgccggc
     7321 caggagcccg gagcgcgtga ggctcaggcg gcgctcgaag ctgccggtgc ggatgcgttt
     7381 cagcgtggtc ttgtcgcgct cggtcatggc cattccgccg cagggacttc gtctaatgta
     7441 ccccatcgcg gctatactgc gcctgcctcc ccgccccccc agccagggaa acgatgtcca
     7501 acgacgacct gctcatccgc gtcgcgctgc tgctcgcggt gagcctcgcg gcgatcgcct
     7561 tggcctggcg cgcgcgccag tacaaccgcc gcctcgcccc cgtgacgcgc ggcacgggca
     7621 tcgccagcgc cggcgcggcg ccggcaccgg cgatcccgcc cggcacgccg tttcgcgacc
     7681 ccatggccga tgcgatgctg cgcgagctga accggtgcgc gtttggcgtc gcccgcttcg
     7741 cccacgcgct gcccgacgag cacgagacga tgctcgagac gctgcgccgc gccgcgctcg
     7801 cacccgtcgc cgagccgccc gcgctgctgg tgccgctgct cgccgccggc ggggatgatg
     7861 cggcgctgca cggcttgctc gacacgctgc ggcgcgatcc cccgctcgcc ggcgcagtgc
     7921 tggaactcgc cgccgacccg gctttcggcc attgcggcga gcccttcacc gggctcgaac
     7981 acgcgctgga ggcgctcggc gccgcgccgc tgcgcgccct gatcgccagc gccctgttgc
     8041 agccggcctt tgcgctgccc gcgcaaccct tcgccgcctt cggccgcgcc gcctggcagc
     8101 tcggcctgca atcggcgctg gcggcagccg ccttcgcgcg cgccggcgca agcggcgacc
     8161 cggtcgcggc gcacgtgctc gggctggtgc acgcactggg cgccgtgacg gtgttccgca
     8221 ctgcgctggc gcactaccag gcgacgcccg gtctggcgcc gcgcgcggaa gtgttcgtgc
     8281 gcctggtcac ggaacgcgcc gacgccgcgg cgcatgcgct ggcggtggcc tggaacctgc
     8341 cggccggcat gcgctcggcg ctcgccgagc acagcgcgca gctcgcgcct ggcgcgatga
     8401 gcgcgccggg acgcgcgcta tatgccgggc ggctgttcgg gctggcggcg gtgctgcgcg
     8461 agcacgacgc gctggaccac gcgggctttg gcgaactgct ggcccgcaag ggcctcgacg
     8521 acgcgcaccg gcagtggcgt ccctccactc ctggcgccgc gcgacaggcg gcgcgatgag
     8581 cacgaagcac acgccgcgcc acggcgcgcg cgacgcggtc tacgccgagg cgcgcgagca
     8641 gatcgccgcc tttcgcttcg atgccgcggt tgccgaggtc ttcgccgaca tgatcgcgcg
     8701 ctcggtgccc ggctacgcgc tcacgctgca actgatcgcg gtcgcggcgc gccggttcgc
     8761 ccaacccggc agccgctgct acgacctcgg ctgctcgctg ggcgcttcca cactggcgat
     8821 ccggcagaat gcacccgcgg cttgccacgt gatcgggatc gacaactccg ccgacatggt
     8881 cgagcgctgc cgcgcgatcg tcgccaccga cccctcgcca gtgccggtgg agatccgctg
     8941 cgaggacgtg tgcgagaccg cactgggcga tgcctcgctg gtcacgctga atttcacgct
     9001 gcagttcatc ccgcccgcgg gccgcgccgc gctgctcgcg cgcatcgcca ccggcatgcg
     9061 tcccggcggc tgcctcgtcc tctccgagaa gctcgccttc gacgaccccg cggaacagga
     9121 actgctgcag acgctgcacc acgacttcaa gcagttgcag ggctacagcg cgctcgaggt
     9181 tgcgcagaag cgcgccgcga tcgagaacgt gctgatcccg gagacgctgg cgacgcatcg
     9241 ccgccgcctg ctcgcggccg gcttcaggcg cgtcacgctc gggctgcagt gcctgaattt
     9301 cgcctcgctg ctggccgaga aatgagcctg ctcgcagggc tcggggatgc gctggcaacg
     9361 gcgccgctgg gcggcgccgt cgcaccgctg ggcgaagcgc tgcagcgctg cctcgcgcag
     9421 ctgcgccacg gcgatgccgg cgactgggag gcggcactgg cggcgctgcc cgcgctgcgc
     9481 ccgaccgacg tcgacctcgg caacgcggcg gtgcgcatcg gcagcgccgc gcagatcgac
     9541 cccgaggccc gcgcacgcct gcacgaggcg ctgcagcaac tgcacccgtg gcgcaagggg
     9601 cccttctcgc tgttcggcat cgatatcgac acggagtggc gctccgacct gaaatgggca
     9661 cgcatcgcgc cgcacctgga tctcggcggg cgccgcgtgc tcgacgtcgg ctgcggcaac
     9721 ggctactacg cgctgcgcat gctgggcgcc ggtgccggct acgtgctcgg catcgacccg
     9781 acactgcgct tcctggcgca gtttcgcgcg ctgcagcact acctcgatga tccgcgcgca
     9841 ctgctgctgc cgctgcgcgc cgaggacctg ccgccgcgcc tgggctgctt cgacacggtg
     9901 ttctcgatgg gcgtgctcta ccaccgccgc tcgcccttcg agcacctcgg cgagctgctc
     9961 gatgcgctgc gccccggggg cgagctggtg ctggaaacac tggtggtcga cggcgatgcc
    10021 gagcgcgtgc tggtgcccgc ggaccgctac gcgatgatgc gcaacgtctg gttcatcccc
    10081 agccccgcgg cgatgctcgg ctggctgcgg cgcgcgggct ttcgcgatgt gcggctggtc
    10141 gatgtcgcgg ccaccacgac gcaggagcag cgccgtacgc cgtggatgcg cttcgagtcg
    10201 ctcgcggatt tcctcgaccc ggcggacccg cggctgaccc gcgagggcta cccggcgccg
    10261 ctgcgcgcgg tgttcatggc cacgcgcgcg cgctgagcac gcatcgggcg cacgcggcat
    10321 gagcgcaccg gcatttcttg atccagatca ttcagcactg caatagcctg gtccatcctc
    10381 cgttcggacg attcatgtcg caagccggca acaaagaatg gcgccacgcc agacaatcat
    10441 gccagccggc accaacagga gagcgcagat gaaccagccc gccacccccc gcaaaccgca
    10501 catcatcgtc gccgacacga tgcccgagcg ctacgcgcgc ggttggcacg tgctcgacaa
    10561 ggcatccgag ttcacgacca agccgcgcat gttcgacgtc ttcggcacgc gcctcgccgt
    10621 gtaccgcggc gacaaggacg ggcaggtcca cgtactcgac gcctactgcc cgcacatggg
    10681 cgccgacctc agcaagggct acgtgaagga cgactcgctg gtctgtccgt tccacgcctg
    10741 gagctggggc ggtgacggcg tgtgcaacga catcccctac gccaagaaga tccccgacaa
    10801 ggccgtgatc aaatcctggc ccgtgctgga gaagaacggc ctgctgttcg tgtggaacga
    10861 cccggagaac aacccgccga tcgccgcgca ggagcccgcg cgcatggaag actactactc
    10921 cggcgcgtgg accgactgga ccatgacgca ggtgccgatc gagaacaatt gccgcgaact
    10981 cgtcgacaac atggcggatc tcggccactt cggccccgtg cactattcgc gcgtcaagag
    11041 cttcaagaac atccaggacg ggcaccgcta catccaggtg atggagggcg gccacgagat
    11101 cctcgaggac ggcaccaccg ggatgaccac gaccgcgatc tacgaaggcc cggcctacat
    11161 gaccaccacg atgaccggca gtctctatgg ccgtccggtg accgtgcacc tgctggtcag
    11221 ccattacccg gtgcacatga acaagttcct catcaacttc ggcgtcatga tgaagaaaga
    11281 cccgaacctg cccgacgtcg tgaaccgcga catggtcgac aagtacacac gcatgagcat
    11341 cgactccttc atccaggacg tcgagatctg ggacaacaag gtgcgcgtgg gcaacccggt
    11401 gctgtgcgac ggcgacggcc cgatccacat gctgcgcaag tggtacagcc agttctacct
    11461 gaacgtggcc gacatcccgg aagacctcgt gaagcacaag gaacacgtgt ctctctgagg
    11521 ccggccacag cgacaggagt ttgcagcgca tgatcgatac caaggcactg ggctatgtcg
    11581 tcatcgagac gaccgacctg gcgaagtggg agcacttcgg cacccgggtg atgggcctga
    11641 tggtggcgcc gtccgtgcag gaggacggcg tgctctacct caagatggac gagtacccgt
    11701 accgctttcg catcgtgcaa ggcgagcagg accgcttcgg ggccgccggc tgggaagtcg
    11761 ccggcccgca ggagttcgcg caggccgtgc gcgaactcga ggcagccggc atcgcttgcg
    11821 agcgcgcccc ggccggggcg tgcaaggcgc ggctgctgag cgagatggtc gccttcaccg
    11881 caccgggcgg cctgcgctgc gaactcttct actacctgca actcgattac ctgccgctgg
    11941 tttcgccggt cggcgtgaag gccttcgtca ccggcttcaa cggcaccatg gggctgggcc
    12001 atatcgccgc agccacgccg cagctggagg cctcgcaccg cttcttcacc gaagtcatgg
    12061 gattcggcca gacggattac ctgcacctgc acttctcgcc cgatccggcg gatcccgggc
    12121 aggggctgca tttcctgcat gcgagcaacc cgcgccacca cagtctcgcg ctgttccagt
    12181 ccaaggatcc gctgcccggg gacctggtgc acctgatggt ggaagtgccg gacctcgaca
    12241 cgctgggtgg cttcatggac cgggtgcact cgaacggggt gcgcgtgatc acctgcctcg
    12301 ggcggcacac caacgaccgc atggt
//
LOCUS       DAORUQ010000099        12153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_5304_length_12153_cov_5.922301, whole genome shotgun sequence.
ACCESSION   DAORUQ010000099 DAORUQ010000000
VERSION     DAORUQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_12153_cov_5.922301"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PLW13_09790"
     CDS             <1..1856
                     /locus_tag="PLW13_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY70710.1"
                     /translation="PVFVTADMQRGAGPRASGGEAGSRYLVGYYHLRVSLDEVWRSVQ
                     GYALRVALACALFVALTALLTLRVTRRISAPLRALEQLVERVSAGNLEQKLIVKGGGE
                     VDRITSTINALISELHTYKLQTDTSNVLLSRKVDERTEQLSRRNEELNKAVQQVTKSK
                     EQLHQLAYYDSLTALPNRRLFIEQLDVLLRLTRREQRIVALLFVDLDNFKRINDSLGH
                     AAGDVMLKEVAGRLSRCLRSSDFLAVGSGGDVPSSVSRLGGDEFTVVLSNIDRPESAG
                     MVAERLLQSLRAPMLIEGHELVVTPSIGIALAPDHADSVSELLKLADTAMYHAKSAGR
                     NNYAYYSPEMSGSGVGRLKLEAELRRALERRELVLHYQPQVKVATGEIVGAEALVRWN
                     HPERGLVPPGQFIPLAEEMGLIIELGAWAVVEACGQIRAFQRQGLTPPKISVNVSSLQ
                     FNAAFTALVQKTIADFGIDPGMLELELTEGVLMSNAEASIRSLHDLKRLGVSLSVDDF
                     GTGYSSLSYLSEFPLDELKIDRSFVIAFDSGPRSASLVSAIIAMGKSLNLRLVAEGVD
                     SGAQYRFLHEAGVDVIQGFLFSKPVPAEDFARLLGSNRFAEQIRTLEQEAA"
     assembly_gap    2035..2134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2135..3392
                     /locus_tag="PLW13_09795"
     CDS             <2135..3392
                     /locus_tag="PLW13_09795"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQY70711.1"
                     /translation="MSKVLRTRPGYFHAAARQARISVQALKVGMRVVELDCPWTDTPF
                     VFQGFRLENVQQIAEVAQYCREVVVEYDEDQWVPPTAHALPGTPARRLRPVVAHAPTV
                     KAYESAARLQDDARALTRSIMDDVRLGRVINVKEIRSTVSAAVKNVIDSPDAVMWLSR
                     IRHRDAYTSEHCVNVGLLAINFARHLGHGEEDLNTIGIAAMLHDVGKTLTPLEVLNKE
                     GALTQEEFELMKAHTTEGRNILMAHRNLSHGPVDVAYGHHEALDGTGYPRKLKASGIP
                     EMTRIVTLCDVYDAVTSDRCYRKGQPSLRGLDVLQRGRGSKYDERLVDEYVRCIGLYP
                     TGSVVELVNGAHGIVLSTNYRNRHLPRVLLVRDEQHEPCAERVLDLQKLSGQGGMEGW
                     LTRAVVPNGAHGIRVEEYVRRGVQIM"
     gene            3403..4047
                     /locus_tag="PLW13_09800"
     CDS             3403..4047
                     /locus_tag="PLW13_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY70712.1"
                     /translation="MGIYERLILPRIIHLACGSSPITKQRLKIVPLASGRVLDVGTGS
                     GHNLPLYDPHRVELVWGLEPNHGMRELARHNVARSRVELHWLDLPGEQVPLHDHSVDT
                     VVLTFTLCTIPDWRAALAQMLRVLKPGGRLLFLEHGAAPDAPVRRWQERMNAGWRRCA
                     GGCNLNRDIPALLREAGFDIVRLETMYLPGTPRIAGYNYWGEAIARCAAPARTC"
     gene            complement(4010..4408)
                     /locus_tag="PLW13_09805"
     CDS             complement(4010..4408)
                     /locus_tag="PLW13_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70713.1"
                     /translation="MSHAHLRNAIAATVLGAALATPAAALPLLGPSCLEKLTAQTRAW
                     AECTERFDRVDNRCRQPAAKMNAYMQECASKGKSKARIDAAMTEGYRQAGNRPVGAKG
                     GTPKPPATTQSPATMENVLSKYAQEPRSAQ"
     gene            4572..6269
                     /locus_tag="PLW13_09810"
     CDS             4572..6269
                     /locus_tag="PLW13_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70714.1"
                     /translation="MAGSGNWAYETDVVVVGSGAGALTAAVRARDLGLEVLVIEKTGQ
                     YGGSSAMSGGVLWLPNSPLVAGAGGSDSAEEGKRYMKSVIDEPHLDKRIDAYVEQIPA
                     FIDFLHRSTHLRLAPLPQFPDMYPDNPDAKMHRCHEAVPFHARALPADEVLNLRPQHP
                     QTALFGLIGWTPSESLILQARGKGWLKVAFSMVARYLLDIPWRLRSRRDRRLVLGGAL
                     VGALRHSMLDRGVPLWLNSPFEDFIEEDGRVAGVLARHDGVRARIRARKGVILAAGGF
                     EKNNALRQQYLDQPTDAAWTAGSPGNTGDTIAAATRLGAKLEFMAEGWWGPTFLVPGE
                     PQARMLIIEKNLPGSIIVNKLGQRFVNESSSYTKVTRGLFAANKPGAESIPAYMIFDA
                     TYRQRYPIGPMLPSTFQPDFAVPGAIKKAIPSAMDIRELARKLGVDPEGLAATVSRFN
                     GFARSGKDEEFQRGDANYDRYYGDQSVGPNSCLGPIEKAPFYGVKIYPGELGTKGGFA
                     VDENARVLREDGSVIEGLFAIGNCSSPVTGTTYPASGSTLGPAMVFGYVAANCIASA"
     gene            complement(6440..7837)
                     /locus_tag="PLW13_09815"
     CDS             complement(6440..7837)
                     /locus_tag="PLW13_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HQY70715.1"
                     /translation="MNNSRRRIVIMGAAGRDFHNFLQVFRDDGNVEVVAFTAAQIPGI
                     AGRRFPASLAGSAYPEGIAIVDEQFLEQLVSEHGVDEVVFSYSDISHLAVMHQASRVL
                     ASGASFALLGPARTMLRSRRPVIAVSAVRTGCGKSQVARWLVQRLHAAGLKSAVIRHP
                     MPYGDLEAQRVQRFASRADLQRADCTIEEREEYEPHIERGATVFAGVDYAAVLAEAER
                     EAEVIVWDGGNNDFPFVRPQLHIVLLDPMRAGDEATHHPGEAVLRMADIALIAKSDTA
                     TPAQIAQLIDSVQALNPRATILRGASPVTLEGAATLAGKRVLVIEDGPTITHGGMAHG
                     AGWRAASDAGALIVDPRRWAGPELQETYRRYPHIGPVLPALGYYAAQIEALRDCIDAC
                     DADAVVSGTPIDLAALLGVNKPVLRARYEFADLESPGLAGALREFLREHGPGQSSRPL
                     PCPPVAPHSCGQLSG"
     gene            8032..>12153
                     /gene="uvrA"
                     /locus_tag="PLW13_09820"
     CDS             8032..>12153
                     /gene="uvrA"
                     /locus_tag="PLW13_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY70716.1"
                     /translation="MEDSIGIEGARQNNLRNLSLELALGELTVITGVSGSGKSSLAFD
                     TIYAEGQRRYVETFSPYARQFLERMDKPQVDAIRGIPPAIAIEQSNPVRTSRSTVGTM
                     TELNDYLKLLYARVATLHCRGCERPVSADSADAVARTLLTDPTLAEQAATVSFRVELL
                     PGMDAAQMTQMLAAQGYTRVSEAGAGMLAVVQDRLRLREENRSRLTESLETAFRLGRG
                     KLAVAAGEPARELRFSNMLHCAQCDIAYAEATPGAFSFNSPLGACDTCRGFGRSIGID
                     YRLVVPDPALSLRKGAIKPWQTESFRECQQDMERYAKKRGIPLDVPWHDLAPAQRDWV
                     VEGEGEIDTGVWYGARRFFAWLESKSYKMHIRVLLSKYRSYELCSACHGARLKPDALL
                     WRIASAAGGERGLDFHAFSKLPVSRALAFVEGLRLPALDDEASAMVLAEIRSRLRFLV
                     EVGVGYLALDRQSRTLSGGEVQRINLTTALGSSLVNTLFVLDEPSIGLHPRDVGRVVT
                     VLERLRDAGNTLLVVEHDPQIMLAADRLIDMGPGPGKEGGDIVFHGPARGIGAAAGSL
                     TGAYLRGERRVERAQRRTVDPAGPCVELLGVSANNLKHIDVRFPLGALVCVTGVSGSG
                     KSTLIGDVLYQAARQQLGRPLELPGAHRELRGLAQLQDVVFVDQSPIGKSARSNPASY
                     VGAWGAIRNLFARTALAAERGYTPGTFSFNAGDGRCPACTGSGFEHVEMQFLSDVYLR
                     CPDCDGKRFRPEVLEVRLEGGCSIAEVLAMTVSEALAQFAAHPEVTAMLAPLVDVGLD
                     YLALGQPVPTLSGGEAQRLKLAGHLAEAGARRRGAKGSLFVFDEPTTGLHFEDIARLL
                     GAFDKLLAAGNSLLVIEHNLDVIDCADWLIELGPEGGEAGGELVFCGTPEAIVALPGC
                     HTGVALAEYRKARGRMPRGRGKRAQRTRPAAAIEIHRAREHNLKDIDLAIPREAFTVI
                     TGISGSGKSTVAFDILFAEGQRRYLESLNAYARQFVQPAVRPDVDSIRGIPPAVAVEQ
                     RTSRGGRKSTVATMTEVHHYLRLLFVKLGTQYCPDCDIPIDAQPAEVIVGRVLREFRG
                     QRVQVLAPLVAGRKGYYTELAKAAARRGQAHLRVDGQMLPTEPWPRLDRYREHWIELP
                     LAVLDVKAREEKRLREAIMEGLAAGNGTVQVLALGGARAGELQGYSTRRSCPRCERSF
                     AELDPRLFSYNSAHGWCASCYGTGLRMEGFDAEQSGEEHFWNDWWQGQEQPCGACHGQ
                     RLRPEALAVRLWDRGPAAYNAMTVDAAERCFATLKLDGREREIARDILPELRSRLGFL
                     RQVGLGYLALERSAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDN"
BASE COUNT         1836 a         4092 c         4277 g         1848 t
ORIGIN      
        1 tacccgtgtt cgtgacggcc gacatgcagc gcggtgccgg gccgcgtgcc tccggcggcg
       61 aggcaggcag ccgctacctg gtcggctatt accacctgcg cgtcagcctc gacgaggtgt
      121 ggcgatcggt gcagggctac gcgctgcggg tcgcgctggc ctgcgccctg ttcgtggcgc
      181 tcacggccct gttgacgctg cgcgtcacgc ggcgcatctc cgcccccctg cgcgcactgg
      241 aacagctcgt cgagcgcgtt tccgccggca acctggagca gaagctgatc gtcaagggcg
      301 ggggcgaggt cgatcgcatc acctcgacga tcaatgccct gatatcggaa ctgcatacct
      361 acaagctgca gaccgatacc agcaatgtgc tgctgagcag gaaggtggac gagcgtaccg
      421 agcagctgtc gcggcgcaac gaggagctca acaaggccgt gcagcaggtc acgaagagca
      481 aggagcagct gcaccagctc gcgtattacg acagtctcac cgcgctgccc aatcgccgcc
      541 tgttcatcga gcagctggac gtgctgttgc gcctgacccg gcgcgagcaa cgcatcgtcg
      601 cgctgctctt cgtcgatctc gacaacttca agcgcatcaa cgactcgctc ggccacgcgg
      661 ccggcgacgt gatgttgaag gaagtggcgg gcaggctctc ccgttgcctg cgcagcagcg
      721 acttcctcgc tgtcggctcg ggcggggacg tgccgtcgag cgtctcgcgc ctcggcggcg
      781 acgagttcac ggtggtgctc agcaacatcg accgccccga gagcgcgggc atggtggcag
      841 agcggctgct gcagtcgctg cgcgcaccga tgctgatcga ggggcacgag ctggtggtca
      901 cgcccagcat cggcatcgcg ctggcgccgg accacgcgga ttcggtgagc gagttgctca
      961 agctcgccga caccgcgatg tatcacgcga agtcggccgg aaggaacaac tacgcctatt
     1021 actcccccga gatgagcggc agcggggtcg gacggctgaa gctggaggcc gaactgcgcc
     1081 gggcgctcga gcggcgcgaa ctggtgctcc actaccagcc ccaggtgaag gtggcaacgg
     1141 gcgaaatcgt cggcgccgag gccctggtgc gctggaatca ccccgagcgc gggctggtgc
     1201 cgccggggca gttcatcccg ctcgccgagg agatgggcct gatcatcgag ctcggcgcct
     1261 gggcggtggt cgaggcctgc ggccagatcc gcgcgttcca gcgccagggt ctgacgccgc
     1321 ccaagatatc cgtcaacgtg tccagcctgc agttcaacgc ggccttcacc gccctggtgc
     1381 agaagaccat cgcggacttc ggcatcgacc ccggcatgct cgaactggag ctcacggagg
     1441 gggtgctgat gagcaacgcc gaggcctcga tccgctcgct gcacgacctg aagcggctgg
     1501 gggtgagcct gtcggtcgac gatttcggca ccggctattc ctcgctcagc tacctcagcg
     1561 agtttccgct ggacgagctc aagatcgatc gcagcttcgt gatcgccttc gacagcgggc
     1621 cgcgcagcgc gagtctcgtc agcgccatca tcgcgatggg caagagcctg aacctgcgcc
     1681 tggtcgccga gggcgtggat tccggcgcgc agtaccgctt cctgcacgag gcgggggtgg
     1741 acgtgatcca ggggttcctg ttcagcaagc cggtccccgc cgaggatttc gcgcggttgc
     1801 tgggcagcaa ccgcttcgcc gagcagatcc gcacgctcga gcaggaggcg gcctgagcgc
     1861 cggctaccct gcgttccgtg acgggggtcg cggaaacggc gcccggcgct ttggctgtta
     1921 cgcgggtgct cgactatctt tcagggggat gcgctgtctg cattgtccac gggatgcccg
     1981 atgtcgaagg tgctcagaac gagaccggga tacttccacg ccgccgccag gcagnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatgtc gaaggtgctc agaacgagac
     2161 cgggatactt ccacgccgcc gccaggcagg cgcgtatttc cgtgcaggcg ctgaaagtcg
     2221 gcatgcgcgt ggtggagctc gactgcccgt ggaccgacac gccgttcgtg ttccagggtt
     2281 tccggctcga gaacgtgcag cagatcgcgg aggtcgcgca gtactgccgg gaagtggtgg
     2341 tcgagtacga cgaggaccag tgggtgccgc cgaccgcgca cgcgctgccc gggaccccgg
     2401 cgcgtcgcct gcggcccgtt gtcgcgcacg ccccgacggt caaggcctac gagagcgcgg
     2461 cccgcctgca ggacgatgcg cgcgcgctga cgcgcagcat catggacgac gttcgcctcg
     2521 ggcgcgtgat caacgtgaag gagatcagga gcacggtttc cgcggcggtg aagaacgtga
     2581 tcgacagtcc cgacgcggtg atgtggctgt ccaggatccg ccaccgcgat gcctacacct
     2641 cggagcattg cgtcaacgtc gggctgctgg cgatcaattt cgcgcgccac ctcggccatg
     2701 gcgaggagga tctcaacacc atcggcatcg ccgcgatgct gcacgatgtg ggcaagacgc
     2761 tcacgccgct ggaggttctc aacaaggagg gcgcgctgac gcaggaggag ttcgagctca
     2821 tgaaggccca caccaccgag ggccgcaata tcctgatggc gcaccgcaat ctcagccacg
     2881 gcccggtcga cgtggcctac gggcaccacg aggcgctcga tggcaccggc tatccgcgca
     2941 agctcaaggc ctcgggcatc ccggagatga cgcgcatcgt cacgctgtgc gatgtctacg
     3001 acgcggtgac ctccgatcgt tgctaccgca agggccagcc ctcgctgcgc gggctggacg
     3061 tgttgcagcg cgggcgcggc agcaagtacg acgagcgact ggtcgatgag tatgtgcgct
     3121 gcatcggcct gtaccccacg ggcagcgtgg tggagctggt gaacggcgcg catggcatcg
     3181 tgctcagcac caactaccgc aaccgtcatc tgccgcgcgt gctgctggtg cgcgacgagc
     3241 agcacgagcc ctgcgccgag cgcgtgctcg acctgcagaa gctttccggg caggggggca
     3301 tggaaggctg gctgacccgg gccgtggtac ccaacggtgc ccacggcatc cgggtcgagg
     3361 aatacgtgcg ccgcggcgtg cagatcatgt agtctgcgcc ccatgggcat ctacgaacga
     3421 ctgatcctcc cgcgcatcat tcacctcgcc tgcggtagct cgccgatcac gaagcaacgg
     3481 ctgaagatcg tgccgctggc ctcgggccgg gtgctcgacg tgggcaccgg atcgggacac
     3541 aacctgccgc tctatgaccc gcacagggtg gaactggtgt ggggcctcga gcccaaccac
     3601 ggaatgcgtg agctcgccag gcacaacgtg gcgcgctcgc gtgtcgaact gcactggctc
     3661 gacctgcccg gcgagcaggt gcccctgcac gaccacagcg tcgataccgt ggtgctgacc
     3721 ttcacgctgt gcacgatccc cgactggcgt gcggcgctcg cgcagatgct gcgcgtgctg
     3781 aaacccgggg ggcgcctgct gttcctggag cacggtgcgg cgcccgatgc gccggtgcgg
     3841 cgctggcagg agcggatgaa tgcgggctgg cggcgctgcg ccggcggctg caacctcaac
     3901 cgcgacatcc ctgcgctgct gcgcgaggcg ggcttcgata tcgtgcggct ggagacgatg
     3961 tacctgccgg gcaccccgcg gatcgcgggc tacaactact ggggcgaggc tattgcgcgc
     4021 tgcgcggctc ctgcgcgtac ttgctgagca cgttctccat ggtggcgggc gattgcgtcg
     4081 tcgccggcgg cttcggggtg ccgcccttcg cgcccaccgg gcggttgccc gcctggcgat
     4141 aaccctccgt catcgcggca tcgatccggg ccttgctctt gcccttgctc gcacactcct
     4201 gcatgtaggc attcatcttc gccgccggct gcctgcaacg gttgtcgaca cggtcgaacc
     4261 gctccgtgca ttcggcccat gcccgcgttt gcgcggtgag cttctccagg cagctcggcc
     4321 cgagcagtgg caacgcggcc gcgggcgtgg caagcgccgc accgagcacg gtggcggcga
     4381 tggcgttgcg caggtgtgcg tgcgacatgc tggaaggtcc gcggtgatga tcccgggcaa
     4441 gtatagccga cgctttagcg tggcaccgtc cggcgcagcc gacctcgtcc gttcgggggt
     4501 actagggtgg atgcgatccg ggttaagctt cgcgccggcg tcaattcgga tcgactgggg
     4561 tggaacaagg catggcgggt agcggcaact gggcatacga aacggacgtg gtggtggtgg
     4621 gatccggcgc cggagcgctg acggcggcgg tacgtgcccg cgatctcggg ctcgaggtgc
     4681 tggtgatcga gaagaccggg cagtacggcg gttcctcggc gatgtcgggc ggcgtgctgt
     4741 ggctgccgaa cagcccgctg gtggcgggcg ccggcggctc ggactccgcc gaggaaggca
     4801 agcgctacat gaagagcgtg atcgacgagc cgcacctcga caagcgcatc gatgcctacg
     4861 tagagcagat cccggcgttc atcgacttcc tgcaccgcag cacccacctg cgcctggcgc
     4921 cgttgccgca gttccccgac atgtaccccg acaacccgga tgcgaagatg caccgctgcc
     4981 acgaggcggt gccgttccac gcccgcgcgc tgccggccga tgaagtgctg aacctgcgcc
     5041 cgcaacaccc gcagaccgcg ttgttcggcc tgatcggctg gacaccgagc gagtcgctga
     5101 tcctgcaggc acgggggaag ggctggctga aggtcgcctt cagcatggtc gcgcgttacc
     5161 tgctcgacat tccgtggcgc ctgcgctcgc gccgcgaccg ccgcctggtg ctgggcggcg
     5221 cgctggtcgg cgcgctgcgc cactcgatgc tggaccgcgg cgtgccgctg tggctgaaca
     5281 gccccttcga ggacttcatc gaggaagacg gccgggtggc cggcgtgctg gcgcgccacg
     5341 acggcgtgcg tgcgcgcatc cgcgcgcgca agggcgtgat cctcgccgcg ggcggcttcg
     5401 agaagaacaa cgcgctgcgc cagcagtacc tcgaccagcc caccgacgcg gcgtggaccg
     5461 cgggcagccc gggcaacacc ggcgacacca tcgcggcggc cacgcgcctc ggcgcgaaac
     5521 tggaattcat ggccgagggc tggtgggggc cgaccttcct ggttcccggg gagccacagg
     5581 cgcgcatgct gatcatcgag aagaacctgc ccggctcgat catcgtcaac aagctgggcc
     5641 agcgcttcgt caacgagagc tcttcgtaca ccaaggtgac gcgcggtctg tttgcggcca
     5701 acaagccggg cgcggaatcc attcccgcct acatgatctt cgatgcgacc tatcgccagc
     5761 gctacccgat cgggccgatg ctgccgagta ccttccagcc cgatttcgcg gtgccggggg
     5821 cgatcaagaa ggccattccg agcgcgatgg acatccgcga gctcgcccgc aagctgggcg
     5881 tcgatcccga gggcctggcg gccaccgtga gccgcttcaa cggctttgcg cgcagcggca
     5941 aggacgagga gttccagcgc ggcgatgcga actacgaccg ttactacggc gaccagagcg
     6001 tggggcccaa ctcgtgcctg ggaccgatcg agaaggcgcc tttctatggc gtgaagatct
     6061 accccgggga actcggcacc aagggcggtt tcgcggtcga cgagaacgcc cgcgtgctgc
     6121 gcgaggacgg cagcgtgatc gagggtctct tcgccatcgg caattgctcc tcgccggtca
     6181 ccggcaccac ctatccggcc tccggctcga cgctgggacc ggcgatggtc ttcggctacg
     6241 tggcagccaa ctgcatcgcc tccgcgtagg tccggattca gagggtgtcg caagagccat
     6301 caggttgcga gcgagcggcc tggtgcgtcg ctggctgtgc tgcccgccat ttcgcgggtt
     6361 tccaccacgg cgaggggccc gcgatgcgcg ggattgtcag gcagctatcc agagcacgga
     6421 tagttttacg acaccctctt cagccggaca attgtccgca tgaatgcgga gctacggggg
     6481 ggcaaggtag cggccggctg ctttgaccag ggccgtgctc gcgcaggaat tcgcgcagcg
     6541 cccccgcgag cccggggctc tcgaggtcgg cgaactcgta gcgcgcgcgc agcaccggtt
     6601 tgttcacgcc gagcagcgcc gcgaggtcga tcggcgtgcc gctcaccacg gcatcggcat
     6661 cgcaggcgtc gatgcagtcg cgcagcgcct cgatctgcgc ggcgtaatag ccgagcgcgg
     6721 gcagtaccgg tccgatgtgg gggtagcgcc ggtaggtctc ctgcaactcc gggccggccc
     6781 atcggcgcgg gtccacgatc agggcgccgg catcgctggc cgcgcgccag cccgcgccgt
     6841 gcgccatgcc gccgtgcgtg atcgtggggc cgtcctcgat caccagcacg cgcttgccgg
     6901 cgagcgtcgc ggcgccctcg agcgtcaccg gtgacgcgcc gcgcaggatc gtcgcgcgcg
     6961 gattcagcgc ctgcacgctg tcgatgagct gcgcgatctg cgcgggcgtg gcggtgtcgg
     7021 acttggcgat cagcgcgatg tcggccatgc gcagcacggc ctcgccgggg tggtgcgtgg
     7081 cctcgtcgcc ggcgcgcatc gggtcgagca gcacgatgtg cagttgcggg cgcacgaacg
     7141 ggaagtcgtt gttgccgccg tcccagacga tcacctcggc ctcgcgctcg gcctcggcca
     7201 gcacggcggc gtaatccacg cccgcgaata ccgtcgcgcc gcgctcgatg tgcggttcgt
     7261 attcctcgcg ctcctcgatg gtgcagtcag cgcgctgcag gtcggcgcgc gaggcgaagc
     7321 gctgcacgcg ctgggcctcg aggtcgccgt agggcatcgg atggcggatc acggcgctct
     7381 tcaggcccgc cgcgtgcagg cgctgcacca gccagcgcgc gacctgcgac ttgccgcacc
     7441 cggtacgcac cgctgagacc gcgatcaccg ggcgcctcga gcgcagcatg gttcgcgcgg
     7501 gaccgagcag cgcgaagctc gcgccgctgg cgagcacgcg cgaggcctgg tgcatcaccg
     7561 cgaggtggct gatgtcgctg taggaaaaca ccacctcgtc gacgccgtgc tcgctgacca
     7621 gctgctccag gaactgctcg tcgacgatgg cgatgccctc ggggtaggcg ctgccggcga
     7681 gcgaggcggg gaagcgtcgt ccggcgatgc ccgggatctg cgccgcggtg aacgccacca
     7741 cctcgacgtt gccatcgtcg cggaacacct gcaggaaatt atggaaatcg cgtccggccg
     7801 cgcccatgat cacgatcctg cgccggctgt tgttcatgct gcttgctctc cgctgctgcg
     7861 cgcgtgtgcg accgcccgga acttagcggc tgcggtagcg gttttgaagc accggatggc
     7921 ctttccttga ccggggtcaa gcgccggcgg cggccgttgc aatgcttccc gcggctcggc
     7981 gaagatgccg ctcgcatccc ccctgcgaca tcaccggatt acgggcacga cgtggaagac
     8041 agcatcggca tcgaaggcgc gcgccagaac aacctgcgca acctcagcct cgagcttgcg
     8101 ctcggggaac tcacggtgat cacgggggtg agcggatcgg gcaagtcctc gctggccttc
     8161 gacaccatct acgccgaggg ccagcgccgc tacgtcgaga ccttctcgcc ctatgcgcgg
     8221 cagttcctcg agcgcatgga caagccgcag gtcgacgcga tccgcggcat cccgccggcg
     8281 atcgccatcg agcagagcaa cccggtgcgc acctcgcgct ccaccgtggg cacgatgacc
     8341 gagctcaacg attacctgaa gctgctgtac gcgcgcgtgg cgacgctgca ctgccgcggc
     8401 tgcgagcgcc cggtgagcgc cgacagcgcc gatgccgtgg cccggacgct gctcaccgac
     8461 ccgacgctgg cggaacaggc cgccacggtg agctttcgcg tcgagctgct gcccggcatg
     8521 gacgctgcgc agatgacgca gatgctcgcg gcgcagggct acacgcgcgt cagcgaggcc
     8581 ggcgccggca tgctcgcggt ggtgcaggat cgactgcggc tgcgcgagga gaaccgctcg
     8641 cggctcaccg agagcctgga gacggcgttt cgcctcgggc gcggcaagct cgcggtcgct
     8701 gccggcgagc ccgcgcgcga gctgcgcttc tcgaacatgc tgcactgcgc gcagtgcgac
     8761 atcgcctacg ccgaggccac gccgggcgcc ttctcgttca actcgccgct cggggcctgc
     8821 gatacctgcc gcggcttcgg acgctcgatc ggcatcgact accgcctggt ggtgcccgat
     8881 cccgcgctga gcctgcgcaa gggcgcgatc aagccgtggc agaccgagag ctttcgcgaa
     8941 tgccagcagg acatggagcg ctacgcgaag aagcgcggca tcccgctcga tgtgccgtgg
     9001 cacgatctcg cgccggcgca gcgcgactgg gtggtggagg gcgagggcga gatcgacacc
     9061 ggcgtgtggt acggcgcgcg gcgcttcttc gcgtggctcg aatcgaagag ctacaagatg
     9121 cacatccgcg tgctgctgtc gaagtaccgc agctacgagc tctgcagcgc ctgccacggc
     9181 gcgcgcctga agcccgacgc actgctgtgg cgcatcgcca gcgctgccgg tggcgaacgc
     9241 gggctcgatt tccacgcttt ctcgaaactg ccggtgagcc gcgcgctcgc cttcgtcgag
     9301 ggcctgcggc tgccggcgct cgacgacgag gccagcgcga tggtgctcgc ggagatccgc
     9361 tcgcgcctgc gtttcctggt cgaggtgggc gtgggttatc tcgcgctgga ccgccagtcg
     9421 cgcacgctct ccggcggcga ggtccagcgc atcaacctca cgaccgcgct cggcagctcg
     9481 ttggtcaaca ccctgttcgt gctcgacgag cccagcatcg ggctgcaccc gcgcgacgtg
     9541 gggcgcgtgg tcacggtgct ggagcgcctg cgcgatgccg gcaacacgct gctggtggtc
     9601 gagcacgacc cgcagatcat gctcgcggcg gaccgcctga tcgacatggg ccccggcccg
     9661 ggcaaggagg gcggcgacat cgtgttccac ggcccggcgc gcgggatcgg ggcggcagcc
     9721 ggctccctca cgggcgccta cctgcgcggg gagcgccgcg tcgagcgcgc gcagcggcgc
     9781 accgtggacc cggcgggtcc gtgcgtggag ttgctcggcg tcagcgcgaa caacctcaag
     9841 cacatcgacg tgcgcttccc gctcggtgcg ctggtctgcg tgaccggggt gagcggttcg
     9901 ggcaagtcga cgctgatcgg cgacgtgctg taccaggccg cgcgccagca actcggccgc
     9961 ccgctcgagc tgcccggggc gcaccgggag ttgcgcgggc tggcgcaact gcaggacgtg
    10021 gtgttcgtcg accagtcgcc gatcggcaag agcgcgcgct ccaacccggc gagttacgtc
    10081 ggcgcctggg gcgcgatccg caacctgttc gcgcgcacgg cgctggcggc cgaacgcggc
    10141 tacacgccgg gcaccttcag cttcaatgcc ggcgacggac gctgcccggc ctgcaccggc
    10201 agcggcttcg agcacgtcga gatgcagttc ctgagcgacg tgtacctgcg ttgcccggac
    10261 tgcgacggca agcgcttccg tcccgaggtg ctcgaagtac gtcttgaggg cggctgctcg
    10321 atcgccgagg tgctggcgat gacggtgagc gaggcgctcg cgcagttcgc cgcccatccc
    10381 gaggtgaccg cgatgctggc gccgctggtc gacgtggggc tcgattacct cgcgctcggt
    10441 cagccggtgc cgacgctctc cggcggcgag gcgcagcgcc tgaagctggc cggccatctc
    10501 gccgaggccg gcgcgcggcg caggggcgcg aagggctcgc tgttcgtgtt cgacgagccg
    10561 acgacaggat tgcacttcga ggatatcgcg cggctgctgg gcgccttcga caagctgctc
    10621 gcggcgggca actcgctgct ggtgatcgag cacaacctcg acgtgatcga ttgcgccgac
    10681 tggctgatcg agctcggccc cgaggggggc gaggccggtg gcgagctggt gttctgcggc
    10741 acgccggagg cgatcgtcgc gctgccgggc tgccataccg gcgtcgcgct cgccgagtac
    10801 cgcaaggcgc gcggacgcat gccgcgcggt cgggggaagc gggcgcagcg cacgcgccct
    10861 gccgcggcga tcgagatcca ccgcgcgcgc gagcacaacc tgaaggacat cgacctcgcg
    10921 ataccgcgcg aagccttcac ggtgatcacc ggcatcagcg gcagcggcaa gagcacggtg
    10981 gccttcgaca tcctgttcgc cgagggccag cggcgttacc tcgaatcgct gaacgcctac
    11041 gcgcgccagt tcgtgcagcc ggcggtgcgc ccggacgtcg actcgatccg cggcatcccg
    11101 cccgccgtgg ccgtcgagca gcgcaccagc cgcggcgggc gcaagagcac ggtcgcgacc
    11161 atgaccgagg tgcaccacta cctgcggctg ctgttcgtga agctcggcac ccagtactgc
    11221 ccggactgcg acatcccgat cgacgcacag ccggcggagg tcatcgtcgg gcgcgtgctg
    11281 cgcgaattcc gcggccagcg cgtgcaggtg ctcgcgccgc tggtggccgg gcgcaagggt
    11341 tattacaccg agctggcgaa ggcggccgcc aggcgcggcc aggcgcactt gcgcgtggac
    11401 ggccagatgc tgccgaccga gccctggccg cggctcgacc gctacaggga gcactggatc
    11461 gagctgccgc tggccgtgct cgacgtcaag gcgcgcgagg agaaacggct gcgcgaggcg
    11521 atcatggagg ggctcgcggc cggcaacggc acggtgcagg tgctcgcgct cggcggcgcg
    11581 cgtgccggcg aactgcaggg ctactcgacg cggcgttcct gtccgcgctg cgagcgcagc
    11641 ttcgccgagc tcgacccgcg gctgttctcg tacaactccg cgcacggctg gtgcgcctcg
    11701 tgctatggca cgggcctgcg catggagggc ttcgacgccg agcagagcgg cgaggaacat
    11761 ttctggaacg actggtggca gggccaggag cagccctgcg gcgcctgcca cggccagcgc
    11821 ctgcgccccg aagcgctcgc ggtgcggctg tgggaccgcg gtcccgcggc ctacaacgcg
    11881 atgacggtgg atgcggccga gcgctgcttc gcaacgctga agctcgacgg gcgcgagcgc
    11941 gagatcgcgc gcgacatcct gcccgagctg cgctcgcgcc tcggtttcct gcgccaggtg
    12001 gggttgggct acctcgcgct ggagcgctcg gccccgacgc tctccggcgg cgaggcgcag
    12061 cgtatccgcc tcgccgcgca gctgggatcg aacctgcgcg gcgtgtgcta catcctcgac
    12121 gagccgacca tcggcctgca cccgcgcgac aac
//
LOCUS       DAORUQ010000100        11510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_5772_length_11510_cov_6.575993, whole genome shotgun sequence.
ACCESSION   DAORUQ010000100 DAORUQ010000000
VERSION     DAORUQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5772_length_11510_cov_6.575993"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1656)
                     /locus_tag="PLW13_09825"
     CDS             complement(40..1656)
                     /locus_tag="PLW13_09825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY70717.1"
                     /translation="MRQLGIIDSAFINLENPTVPQHIGGLGIYDPSTAPGGFVRFKDV
                     LAGFERRLARMPIFRTRLVEVPFGLDRPYWVLDPHFDVEFHIRHIALPKPGDWRQLCI
                     QIARLHSRPIDMSRPLWEAYIIEGLDNIPDLPKGSFAVYTKMHHSLVDGGGGANFMAA
                     IHDLEPVPPAPDESRDPPARFGDLQPSDVRLISKSLLRTGTRLVSTVRGTGRLLGEFT
                     SMARRILNEELPPAQLSSPKTRFDQPVGHNRVFEATQFTMDEIKALKNAANVTVNDIC
                     VAIVAGAMRQYLLAHNELPDESLAASMPVNMRSRVGETQDNNQVGAMQAQLHTNIKDP
                     LERLAAIKKSLDAAKAYIDTPLVRIVALPGALPPLIAKPLARTYVRNKLTRYLPMGQA
                     TVITNVPGPPFDLYCAGARMVTYYPLGLINPGLGLFHAIFSCAGKVAISITGDRDQMP
                     DPEFYRECLDASYDELRKALLGTGVPALAAKATAKRTAKVVRKAEAKATRAAKPRAKA
                     KPQAKAKAKAKAKAKAKAKAAPKRARRSQP"
     gene            complement(1740..3206)
                     /locus_tag="PLW13_09830"
     CDS             complement(1740..3206)
                     /locus_tag="PLW13_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY70718.1"
                     /translation="MKRLGIIDSAFINMENPTVPQQIGSLGIYDPSTAPGHFVRFKDV
                     LASFERRLQRLPLFRTRLVEVPFGLDRPYWVLDPAFDVEFHIRHIALPHPGDWRQLCI
                     QVARLHARALDMSRPLWEVYVIEGVDRIPGLPKGSFAVYTKMHHSMVDGAGSQNFMAA
                     IHDLEPSPPKDAPLLLPAKSQLPDISATDVHLLGRTLLNALPDTVARVRATMDVAGRL
                     TRMAVKMARKELPPAQMQGPKTRFDAPVGPNRVFEARLFDLEDLKALKNAAGMTLNDV
                     AVAIVAGALRQYLLAHGELPRESVLGSMPVNMRTRVGETGDNNQVGAMTAKIHTDIAD
                     PIERLHAIKKSLDEAKAYIDTPLVEMSKVMGMLPPLLSKPIAATYVRNQLTRLLPLGV
                     ATVITNVMGPPFPVYCAGAQLVQFYPLGLLNPGLGLFHAVFSMSGNVSITVTADRDQM
                     PDPEFYGECIAASYEELRDALLAKPAPPRRKSVGRASA"
     gene            complement(3259..4479)
                     /locus_tag="PLW13_09835"
     CDS             complement(3259..4479)
                     /locus_tag="PLW13_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70719.1"
                     /translation="MVDVIKLGIKRPVTRPLLPDPEPRPVKYTLISADDHLMEPPHTF
                     EGRLPAKLQERAPRIVETEEGHQVWSIEGKPYFQVGFMCVAGRSREDQRMEPARFDEI
                     RAGCWDADARIRDMDIGGIWASVNFPSGVTGFGGHLFSQFQDQELGLACVRAWNDWLF
                     EEWYSKHPERIVPLGITHLTDPVKGAEEIRRNARRGFKAVTMPEQPHNVGLPPVFDTA
                     HWDPIIRACAETGTVLNLHVGSAGFAKMPPGAPLLELGATLFGPMALTSCAEWLWSGF
                     PARYPELKIAMSEGGIGWAAMLMDRLDDIMSRSGYGAGWPDKKNSPSEVLKRNFWFCM
                     IEDASTICTRHRIGVENILFESDYPHGDGTWPDTQKVIERVLGALPVEEIRMITHANA
                     AKLYRHPLPAKIVP"
     gene            complement(4494..5381)
                     /locus_tag="PLW13_09840"
     CDS             complement(4494..5381)
                     /locus_tag="PLW13_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70720.1"
                     /translation="MATDIGRLGVWSFIDNLSAPEAAAFAQQLEAWGYSALWIPEAVN
                     RDPFAIIGYLAAKTSKLILATGIANIYARDPMTMKAIRKTLAELAPGRIVIGLGVSHS
                     HLVHNVRGHEYSNKPVSTMRAYLEGMEKALYMGKEPAEEAPLVLAALRQNMLKLAAEK
                     TQGAHPYFVTPEHTARARATMGPDAWLCPEQMVLLETDPAKARAVARKNMGIYLGLPN
                     YQNNLREYGFGNADFADGGSDRVIDAICAWGDEATIRARIKAHHDAGATHVCIQAFRT
                     DGEMGPDLRTLELLAPNRK"
     gene            complement(5528..6106)
                     /locus_tag="PLW13_09845"
     CDS             complement(5528..6106)
                     /locus_tag="PLW13_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HQY70721.1"
                     /translation="MQITDIIARMGGLQSMARELGVSEAQAANGAEALLPAILGGFKK
                     QAQSQPTGLEGLGGLLSQLGGGGLLDEVLAPRATNLEHGNDVLGQIFGSRDVSRTVAQ
                     NAASQSGLDPALLKKMLPMLAMLVAGFMAKQGDTGSVTQAAPAGGGLGDLLGSLLGGQ
                     TGGAPGIASMLDLNGDGNPLDDILRMVGQARR"
     gene            complement(6143..6538)
                     /locus_tag="PLW13_09850"
     CDS             complement(6143..6538)
                     /locus_tag="PLW13_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HQY70722.1"
                     /translation="MGFFSKILAKLGIGQAQAAPAPEPAPAAAPVSTAPVAPPAPTPI
                     AMVDVVAQLEQRAAANPQKLNWRTSIVDLLKLLEIDSSLAARKELAIELGCPADLMDD
                     SAQMNMWLHKTVLKRIADNGGNVPPDLLD"
     gene            complement(6709..7038)
                     /locus_tag="PLW13_09855"
     CDS             complement(6709..7038)
                     /locus_tag="PLW13_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70723.1"
                     /translation="MLERLRAKKQSPRPALGLAWYDEANWAMVKATALDPHRLENSFA
                     EWLAMATQTLDHLKRSGADATPVHIVAAELIAWCRACRRQNDAAARAAYVAARMRVPK
                     KPAEKLP"
     gene            complement(7189..7458)
                     /locus_tag="PLW13_09860"
     CDS             complement(7189..7458)
                     /locus_tag="PLW13_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HQY70724.1"
                     /translation="MAIELPKEIRTRAVGSIQRYFRENMEEPIGNMDAEALLDFFLQE
                     IGPLAYNKAVADVQERLQLRVSEIDLEVHEDEFPYWHKSGRQGRK"
     gene            complement(7475..9265)
                     /locus_tag="PLW13_09865"
     CDS             complement(7475..9265)
                     /locus_tag="PLW13_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQY70725.1"
                     /translation="MPDVDLSLRPPRPGARRALAALCLLAFAHTSAAQPGVIRGFGDS
                     LLDTGRVCARTGFPSGDYGTCSNGKGTLQWLPDYAPLVFDRSTNLAEGGAGTGAFNVA
                     SLIVPDAAGAATQVQRFVTSGARIGATDLVVYSAGPNNRYLLGPAGPLFGSQYNPALS
                     GEGLAERSLAETHASIGALIDAGGRNVVVFGGRARTVEGSPEQRYMAAMNNGLAQALN
                     GFAGEGVRLRIFDFQSIYLRAITDPQRYGLDEPFLYSDYIHPTEAGHRLFARYIAALI
                     ASADAIAAQVDTAEAATHAFSDAVLERLQAVRPVASASQSGFGVYLQPLAVRGERADF
                     FSADGAATGFDYAVDGLLAGIEYRDGAQTRYGATLGYAGADTDLDDSAGTSDTSTTQG
                     SVYLSHDRETLFADAILAYADHEIDIERRGVIDTLVADTGGRSALAGARAGYLFQHGN
                     VRVGPLLTLAYTDTGIDGYSERGDDVLAQRVGDQGLESLEIGAGIRLRAALRLAGCAV
                     EPALDMSLVNDVMADERRLRTANVFAPSLPVYTLIDDDEREDRFARIAAGVGFALTQD
                     LALTLAAKARLPHEGNHEYGVSASLELALP"
     gene            9432..10667
                     /locus_tag="PLW13_09870"
     CDS             9432..10667
                     /locus_tag="PLW13_09870"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY70726.1"
                     /translation="MRSVVFSLWALFAGLSLIIVGNGLQSTLLGVRASLSGFPTGVTG
                     AVMACYYLGFMLGSWTTPRLIARVGPIRVFAALASLFSFAPLAHALFVDPVAWAIVRL
                     LSGACIVGIYIIVESWLNAEATNANRGRLLSLYLIVSQGSVAAGQFLLQAADPLGFEL
                     FVLISALGSLAVVPLMLTRHPAPRHEQHAAISLRALTRRVPLTVVGILLVSLSYGAFY
                     AIGPVYALEIGFEPSQIAWFMAAAIVGSTLLQWPIGMLSDRMDRRRVLVGLSAMVSVI
                     AAALALMPGHAALPIYALMFLYGGLSFPLYGVFVSLAGDQMRGDELVAASSKVLLVNG
                     AGSAIGPVLVAWLMDAVDARAYLLFVALIHVIVCALVVYNLLRTGTGTVVPVTHHVNA
                     TPQGSVVAAEMAGRVAGEP"
     gene            10690..>11510
                     /locus_tag="PLW13_09875"
     CDS             10690..>11510
                     /locus_tag="PLW13_09875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY70727.1"
                     /translation="MNEATRPLAGLRVLELGQLLAGPFTASILAYFGAEVIKVEPPEG
                     DPIRGWRQLDEGGTSYWWRSLGRNKKCISVDLKKARGQDMVRRLAERSDVLIENFSPG
                     VLERWGLAPGVLKAINPRLVIARISGYGQDGPYASKPGFASVCEGISGFRYLNGFPGE
                     APVRPNLSIGDTIAALHAALGILIALRARDALPGAGGQVVDVALYESMFNLMEAVVPE
                     YSGAGLIREPAGTTVTGIVPTNTYRCADGRFVVIGGNGDSIFRRLMEGIGRQDLA"
BASE COUNT         1650 a         3743 c         4026 g         2091 t
ORIGIN      
        1 tgaatgccga tccaagccca cggttcggta aagccgaacc tacggttgtg accgtcttgc
       61 gcgcttgggc gccgcctttg cctttgcctt tgcctttgcc tttgcctttg cctttgcctt
      121 cgcctgtggt tttgccttcg cccgtggctt cgccgcacgc gtggctttgg cctctgcctt
      181 gcgcactact ttcgccgtgc gcttcgcggt cgcctttgcc gcgagggctg gcacgcccgt
      241 gcccagcagc gccttgcgca gttcatcgta ggaggcgtcg aggcactcgc ggtagaactc
      301 cgggtccggc atctggtcgc gatcgccggt aatcgatatc gcgaccttgc cggcgcagct
      361 gaagatcgcg tggaacaggc ccaggcccgg gttgatgagt cccagcgggt agtaggtgac
      421 catgcgcgcc ccggcacagt agagatcgaa gggcgggccg ggcacgttgg tgatcaccgt
      481 ggcctgtccc atcggcaggt agcgcgtgag cttgttgcgc acgtacgtgc gcgcgagcgg
      541 cttggcgatc agcggcggca gggcgcccgg tagcgccacg atcctgacca gcggcgtgtc
      601 gatgtaggcc ttggctgcgt cgaggctctt cttgatcgcg gccaggcgct cgagcgggtc
      661 cttgatgttg gtgtgcagct gcgcctgcat ggcgccgacc tggttgttgt cctgtgtctc
      721 gccgacgcgt gaacgcatgt tcaccggcat cgaggcggcg agtgactcgt ccggcagctc
      781 gttgtgcgcg agcaggtact ggcgcatcgc gcccgcgacg atcgcgacac agatgtcgtt
      841 caccgtgacg ttggcggcgt tcttcagcgc cttgatctcg tccatcgtga actgcgtcgc
      901 ctcgaacacg cggttgtggc ccacgggctg gtcgaagcgc gtcttcgggc tcgagagctg
      961 ggcgggcggc agctcctcgt tcagtatgcg cctggccatc gaggtgaact cgccgaggag
     1021 ccgccccgtg cccctcacgg tcgacaccag tcgcgtgccg gtgcgcagca gcgacttgct
     1081 gatcaggcgc acgtcggagg gctgcaggtc gccgaagcgt gccggtgggt cgcgcgattc
     1141 gtccggcgcg ggcggcacgg gctcgaggtc gtgtatcgcg gccatgaagt tggccccgcc
     1201 gccgccgtcg accagtgaat ggtgcatctt ggtgtagacg gcgaaggaac ccttcggcag
     1261 gtccgggatg ttgtcgaggc cctcgatgat gtacgcctcc cacagcgggc ggctcatgtc
     1321 tatcgggcgc gagtgcagcc gcgcgatctg gatgcagagc tggcgccagt cgcccggttt
     1381 gggcagcgcg atgtggcgga tgtggaattc cacgtcgaag tgcgggtcga gcacccagta
     1441 cgggcggtcg agaccgaacg gtacctcgac caggcgggtg cggaatatcg gcatcctcgc
     1501 cagccgccgc tcgaagccgg ccagcacgtc cttgaaacgc acgaagccgc ccggagcggt
     1561 cgaggggtca tagatcccga ggcccccgat atgttgcggc accgtcgggt tttccagatt
     1621 gatgaacgcg gagtcgatga tgccgagctg gcgcatgcgg attgtcccgg gtaagagcct
     1681 gttgcgagcg agtgtacaga caccaagtgg cgcgtgtcat gaggcacggg aattgtgggt
     1741 caggctgaag cccgacccac ggacttgcgg cggggcgggg cgggtttggc gagcagcgca
     1801 tcacgcagct cttcatagct ggcggcgatg cactcgccgt agaactcggg gtccggcatc
     1861 tggtcgcggt cggccgttac cgtgatgctc acattgccgg acatgctgaa caccgcgtgg
     1921 aacaggccga ggccggggtt cagcaggccc aacgggtaga actgcaccag ttgcgcgccg
     1981 gcgcagtaca cggggaaggg cggtcccatg acgttggtga tcaccgtcgc cacgccgagc
     2041 ggcagcagcc tggtcagctg gttgcgcacg taggtcgcgg cgatgggctt cgacagcagc
     2101 ggtggcagca ttcccatgac cttcgacatc tcgaccagcg gcgtgtcgat gtaggccttg
     2161 gcctcgtcca ggctcttctt gatcgcgtgc aggcgctcga tcgggtcggc gatgtcggtg
     2221 tggatcttcg cggtcatcgc gccgacctgg ttgttgtcgc cggtctcgcc cacgcgggtg
     2281 cgcatgttca ccggcatcga gcccagcacc gactcccggg gcagctcgcc gtgcgccagc
     2341 aggtactggc gcagcgcacc cgcaacgatg gccaccgcca cgtcgttcag cgtcatgccg
     2401 gccgcgttct tgagcgcttt caggtcctcg agatcgaaca gccgcgcctc gaacacgcgg
     2461 ttcggcccca cgggcgcatc gaaacgcgtc ttcggtccct gcatctgcgc gggcggcagc
     2521 tccttgcgtg ccatcttcac tgccatgcgt gtcaggcggc ctgccacgtc catggtggcg
     2581 cgcacgcgcg cgaccgtgtc gggcagggca ttcagcagtg tgcggccgag caggtgcacg
     2641 tcggtggcgc tgatgtcggg gagctgcgac ttggccggca gcagcagcgg cgcgtccttc
     2701 ggcggcgagg gctcgaggtc gtgtatcgcg gccatgaagt tctggctgcc ggcgccgtcg
     2761 accatgctgt ggtgcatctt ggtgtagacc gcgaaggagc ccttcggcag ccccggaatg
     2821 cggtccacgc cctcgatcac gtacacctcc cacagcgggc ggctcatgtc gagcgctcgc
     2881 gcgtgcaggc gcgcaacctg gatgcacagc tggcgccagt cgccggggtg cggcagcgcg
     2941 atgtggcgga tgtggaattc cacgtcgaag gcgggatcca gcacccagta cgggcggtcg
     3001 aggccgaacg gcacttccac cagccgcgtg cggaacagcg gcaggcgctg cagccgccgc
     3061 tcgaagctgg ccagtacgtc cttgaagcgc acgaaatggc ccggcgccgt cgaggggtcg
     3121 tagatcccca ggctgccgat ctgctgcggc accgtggggt tctccatgtt gatgaatgcc
     3181 gagtcgatga tgcccagtct tttcatgtgt gctgtctccg cagccttgga cgtttcggtc
     3241 gccccgatat cgatgcgctc agggcacgat cttcgccggc agcgggtggc ggtagagctt
     3301 cgccgcattg gcatgcgtga tcatgcggat ctcctcgacc ggcagcgcgc ccagcacgcg
     3361 ctcgatcacc ttctgcgtat cgggccaggt gccgtcgccg tgcgggtagt ccgattcgaa
     3421 caggatgttc tcgacgccga tgcgatggcg cgtgcagatg gtcgaggcat cctcgatcat
     3481 gcagaaccag aagttgcgtt tcagcacctc gctcggcgag ttcttcttgt cgggccagcc
     3541 cgcgccgtag cccgagcggc tcatgatgtc atcgaggcga tccatcagca tggcggccca
     3601 gccgatgccg ccctcggaca tcgcgatctt cagttccggg tagcgcgccg ggaaaccgct
     3661 ccacagccac tcggcgcagc tggtgagcgc catcggcccg aacagcgttg cgcccagttc
     3721 cagcagcggc gcgccgggcg gcatcttcgc gaagccggcg gaacccacgt gcaggttgag
     3781 cacggtcccg gtctcggcgc aggcgcggat gatcgggtcc cagtgcgcgg tgtcgaacac
     3841 cggcggcagt cccacgttgt gcggttgctc gggcatggtc acggccttga agccgcgcct
     3901 ggcgttgcga cggatctcct cggcgccctt caccggatcg gtgagatgcg tgatgcccag
     3961 cggcacgatg cgctcggggt gcttcgagta ccactcctcg aacagccagt cgttccaggc
     4021 gcgcacgcag gcgagcccca gttcctgatc ctggaactgc gagaacaggt gcccgccgaa
     4081 gccggtcacg cccgagggga agttcaccga ggcccagatg ccgccgatgt ccatgtccct
     4141 gatccgcgca tccgcgtccc agcacccggc gcggatctcg tcgaaacgcg cgggctccat
     4201 gcgctggtcc tcgcgcgagc ggcccgccac gcacatgaaa cctacctgga agtagggctt
     4261 gccctcgatc gaccacacct ggtggccttc ctcggtctcc acgatgcgtg gcgcgcgctc
     4321 ctgcagcttc gccggcaggc ggccctcgaa ggtgtgcggc ggctccatca ggtgatcgtc
     4381 cgcggagatc agcgtgtact tgaccgggcg cggctcgggg tcgggcagca acggcctggt
     4441 gaccggacgc ttgatgccga gcttgatgac gtcgaccatg gttgaatccc cggttatttg
     4501 cggttcggcg cgagcagttc cagcgtgcgc aggtccgggc ccatctcgcc gtccgtgcgg
     4561 aaggcctgga tgcatacgtg cgtggcgccg gcgtcatggt gcgcctttat gcgggcgcgg
     4621 atcgtggcct cgtcgcccca ggcgcagatc gcatcgatca cgcggtcgct gccgccgtcg
     4681 gcgaagtccg cgttgccgaa gccgtactcg cgcaggttgt tctggtaatt gggcagcccc
     4741 aggtagatac ccatgttctt gcgcgcgacc gcgcgtgcct tcgccgggtc ggtctccagc
     4801 agcaccatct gctcggggca cagccaggca tccggcccca tcgtggcgcg cgcgcgcgcg
     4861 gtgtgctcgg gcgtgacgaa gtacgggtgt gccccctggg tcttctcggc ggccagcttc
     4921 agcatgttct ggcgcagcgc cgccagcacc agcggtgctt cctcggcggg ctccttgccc
     4981 atgtacaggg ctttctccat gccttccagg taggcgcgca tggtcgagac gggcttgttc
     5041 gagtactcgt gcccgcgcac gttgtgcacc aggtgcgagt gcgagacgcc gagcccgatc
     5101 acgatgcgcc cgggcgcgag ctccgccagc gtcttgcgaa tcgccttcat cgtcatcggg
     5161 tcgcgggcat agatgttcgc gatgcccgtg gccaagatga gcttgctggt cttcgccgcc
     5221 aggtaaccga tgatcgcgaa ggggtcgcgg ttcacggcct cgggaatcca cagcgcggaa
     5281 tagccccagg cttcgagttg ctgcgcgaag gccgctgcct ccggcgcgga aaggttgtcg
     5341 atgaacgacc agacgcccag gcggccgatg tcggttgcca tgtcctgctc cctgttgtgc
     5401 tgtcgatgag gctggggggg ccgagtgtat ccaatgcccg ggccggcttc catgacggct
     5461 cgtaaatgtc tgcggggagg agtagttcgg ctggaggccc cccgcagcta gtgcgggggg
     5521 tagtcgttca gcgcctcgcc tggccgacca tgcgcaggat atcgtcgagc gggttgccgt
     5581 cgccgttcag gtccagcatc gaggcgatcc ccggtgcgcc gccggtctgg ccgccgagca
     5641 ggcttcccag caaatcgccg agccctccgc ccgctggcgc ggcctgcgtc acgctgcccg
     5701 tgtcgccctg cttcgccatg aagcccgcga ccagcatggc cagcatgggc agcatcttct
     5761 tcagcagcgc ggggtcgagt cccgactgcg aggccgcatt ctgcgcaacc gtgcggctca
     5821 cgtccctgga gccgaagatc tgtccgagca cgtcgttgcc gtgctccagg ttggttgccc
     5881 gtggcgcgag cacctcgtcg agcagcccgc ccccaccgag ctgcgagagc agcccgccga
     5941 gcccttccag ccccgtgggc tgcgactgtg cctgcttctt gaacccgccg aggatggcag
     6001 gcagcagggc ctcggccccg ttcgcagcct gggcttcgct gacgccgagt tcccgtgcca
     6061 tcgactgcaa gccgcccatc cgggcgatga tgtccgtgat ctgcatgagc gtgactcctt
     6121 cgcgtggtgt ggcggttcct gctcagtcga ggagatccgg gggcacgttg ccgccgttgt
     6181 cggcgatgcg cttgagcacg gttttgtgca gccacatgtt catttgtgcc gaatcgtcca
     6241 tcaggtcggc cggacagccc agctcgatgg ccagttcctt gcgcgccgcg aggctgctgt
     6301 cgatctcgag cagcttgagc aggtcgacga tggacgtgcg ccagttgagc ttctgcgggt
     6361 tcgccgcggc acgttgctcg agttgcgcga ccacgtccac catcgcgatc ggggtgggcg
     6421 cgggcggtgc aacaggagcc gtgctcaccg gggcggcggc gggtgccggt tcgggtgcgg
     6481 gggcggcttg cgcctggccg atgccgagtt ttgcgaggat cttgctgaag aatcccatga
     6541 agtctctcct gatcttgatt ggtagtggcg cgagcatacc gcattgcggc tgctacgcct
     6601 cgctcggccc gaatatgccg gattccgtca cgggcggaag atttgcgccg acattggccc
     6661 gcgcggggtc gaaaacggta atggcgaact gattcaacgc ggcgcggatc atggcagctt
     6721 ttctgcaggc tttttcggga cccgcatgcg tgctgcgacg tacgcggcac gcgccgcggc
     6781 gtcgttctgt ctgcggcagg cgcggcacca ggcgatcagt tccgccgcga cgatgtgcac
     6841 cggcgtggca tccgcgccgg agcgcttcag gtggtccagc gtctgggtgg ccattgcgag
     6901 ccactcggcg aaggagttct ccaaacggtg gggatcgagc gccgtcgcct tcaccatcgc
     6961 ccagttggcc tcgtcgtacc aggcgagccc cagcgcgggt cggggcgatt gcttcttggc
     7021 gcgcagtcgt tcgagcaagc tcggtttgtg catggacgac tcccctcagg atgttcgccg
     7081 cggattctag ccgccgattc gagcccgcgt catgcgctcc ggctgcctgt ctgtgcgact
     7141 cgcccacgac cgaccccccg gcatgaaaga agcccgctcc cggtctgatc attttctgcc
     7201 ctgccgcccg gacttgtgcc agtacgggaa ttcgtcttcg tgcacctcga gatcgatttc
     7261 cgagacgcga agctgcagac gttcctgcac gtctgcgacc gccttgttgt aggcgagcgg
     7321 gccgatctcc tgcaggaaga aatcgagcag ggcctcggcg tccatgttgc cgatcggctc
     7381 ttccatgttt tcgcggaaat agcgctggat ggatccgaca gcccgggtcc ggatttcctt
     7441 ggggagttcg atggccatga aattcctgtt cctgtcaggg cagcgccagc tcgaggcttg
     7501 cgctgacgcc gtactcgtgg ttgccctcgt gcggcaggcg cgccttggcg gccagcgtca
     7561 gcgcgaggtc ctgcgtgagc gcgaagccca cgccggcggc aatgcgcgcg aagcggtcct
     7621 cgcgctcgtc atcgtcgatg agcgtgtata ccggcagcga cggagcgaag acgttcgccg
     7681 tgcggagcct gcgctcgtcg gccatcacgt cgttcaccag gctcatgtcg agcgccggct
     7741 ctaccgcgca tccggcgagg cgcagcgccg cgcgcaggcg gatccccgca ccgatctcca
     7801 gcgattcgag gccctggtcg ccgacgcgct gcgccagcac atcgtcgccg cgttcggagt
     7861 agccgtcgat cccggtgtcg gtgtaggcga gcgtgagcag cgggccgaca cggacattgc
     7921 catgctggaa caggtaaccc gcgcgcgcgc cggccaaggc gctgcggccg ccggtatccg
     7981 cgaccagcgt gtcgatcacg ccgcgccgct cgatgtcgat ctcgtgatcg gcataggcca
     8041 ggattgcatc ggcgaacagg gtctcccgat cgtgcgatag gtagacgctg ccctgcgtgg
     8101 tcgaggtgtc ggaggtgccg gcggaatcat cgagatcggt atccgcgccg gcgtagccga
     8161 gcgtcgcgcc gtagcgcgtc tgtgcgccgt cacggtactc gatgccggcg agcagtccgt
     8221 ccaccgcgta atcgaaaccg gttgccgcgc cgtcggccga gaaaaaatcc gcgcgctcgc
     8281 cgcgcaccgc aagcggctgc aggtagaccc cgaaacccga ttgcgacgcg gacgcaacgg
     8341 ggcggaccgc ctgcagccgc tcgagcacgg catcgctgaa cgcgtgcgta gccgcctcgg
     8401 cagtgtcgac ctgtgccgca atggcgtcgg cggaagcaat gagggcggcg atgtagcgcg
     8461 cgaacaggcg atgcccggct tcggtggggt ggatgtagtc gctgtagagg aacggctcat
     8521 cgagtccgta gcgttgcggg tccgtgatgg cgcgcaggta gatgctctgg aaatcgaaga
     8581 tgcgcagccg cactccttct cccgcgaagc cgttcagtgc ctgcgcaagc ccgttgttca
     8641 tcgcggccat gtagcgctgc tccggggagc cttcgacggt gcgcgcgcgt ccgccgaata
     8701 ccaccacgtt gcggccgccg gcatcgatca gcgccccgat gctggcatgc gtctcggcga
     8761 gtgagcgctc cgcgaggccc tcgccgctca gtgccgggtt gtactgcgag ccgaacagcg
     8821 ggcccgccgg cccgagcaga tagcgattgt tgggcccggc cgaatacacc acgaggtcgg
     8881 tcgcgccgat gcgggcaccc gaggtgacga agcgctgcac ctgcgtggcg gcgcccgcgg
     8941 cgtcgggaac gatcagcgac gccacgttga atgcgccggt gcccgcgccg ccctcggcga
     9001 ggttggtgga gcgatcgaat accagtggcg cgtaatcggg caaccattgc agtgttccct
     9061 tgccgttgct gcaggtgccg tagtcgccgg acggaaatcc ggttcgcgcg cagacgcgcc
     9121 cggtgtcgag caggctgtcg ccgaagcccc ggatcacgcc gggttgcgct gcgctcgtgt
     9181 gcgcaaatgc gagcaggcac agggcagcca gcgcgcgccg cgcgccgggt cgcggcgggc
     9241 gcagcgatag atctacatcg ggcatgagcg cgatccctga attcgcattg gcaactcgcc
     9301 agcagttcat tctttcaggc ctgcgccccg agcgccaatc cggaatcgcc gtcgtagtcc
     9361 agcaccgcga ttctggccac cccccatgcc cgctgctagc atgccgtctt cagaaacagg
     9421 tcgttcgtgt catgcgcagc gtcgtattct ccctgtgggc actgttcgcg gggctttcgc
     9481 tcatcatcgt gggcaacggg ctgcagagca cgctgctcgg ggtgcgcgcg agcctgtcgg
     9541 gtttcccgac cggtgtcacc ggcgcggtga tggcctgcta ctacctcggc ttcatgctgg
     9601 gctcctggac caccccgagg ctcattgcgc gggtcgggcc cattcgcgtg ttcgcggcgc
     9661 tggcctcgct gttctccttc gccccgctgg cgcacgccct gttcgtggat ccggtggcgt
     9721 gggccatcgt gcgcctgctg agcggtgcct gcatcgtcgg catctatatc atcgtcgaga
     9781 gctggctgaa cgccgaggcc accaacgcga accgcggccg gctgctctcg ctgtacctga
     9841 tcgtcagcca gggctcggtg gcggcggggc agttcctgtt gcaggcggcg gatccgctcg
     9901 gcttcgagct cttcgtgctg atctcggcgc tgggctcgct ggcggtggtg ccgctgatgc
     9961 tcacgcgcca tccggcgcca cgtcatgagc agcacgccgc catcagcctg cgggctctca
    10021 cgcgccgggt gccgctcacg gtcgtgggca tcctgctggt ctcgctgagc tacggcgcct
    10081 tctatgcgat cgggccggtg tatgcgctgg agatcggctt cgagccatcg cagatcgcgt
    10141 ggttcatggc ggcggccatc gtcggcagca cgctgttgca gtggcccatc ggcatgctgt
    10201 cggaccgcat ggaccggcgc cgcgtgctgg tgggcctgag cgccatggtc agcgtgattg
    10261 ccgcagcgct ggcgctgatg cccgggcacg cggcgttgcc catctacgcg ctgatgttcc
    10321 tctacggcgg cttgtccttt ccgctgtacg gggtgttcgt gtcgctggcc ggcgaccaga
    10381 tgcggggcga cgaactggtt gccgccagca gcaaggtgct gctggtgaac ggggcgggct
    10441 cggccatcgg gccggtgctg gtggcctggc tgatggacgc ggtggacgcg cgtgcctacc
    10501 tgttgttcgt ggcgcttatc cacgtgatcg tgtgcgcgct ggtcgtatac aacctgctgc
    10561 gaaccggtac gggcacggtg gtgccggtca cgcatcacgt gaacgcgacg ccgcagggtt
    10621 cggtggtggc ggcggagatg gccgggcgcg tggcgggtga accctgatcg gggagtgtcg
    10681 gaggcatcga tgaacgaagc gaccaggccg ctggccgggc tgcgggtgct ggaactgggg
    10741 caactgctgg cgggaccgtt cacggcgtcg atcctggcgt atttcggcgc cgaggtgatc
    10801 aaggtcgagc cgcccgaggg cgacccgatc cgcggctggc gccagctcga cgagggcggc
    10861 acctcgtact ggtggcgcag cctcgggcgc aacaagaagt gcatcagcgt cgacctgaag
    10921 aaggcgcgcg gccaggacat ggtgcggcgc ctcgcggagc gctccgacgt gctgatcgag
    10981 aacttcagtc ccggcgtgct ggagcgctgg gggctggcgc ccggggtgct gaaggcgatc
    11041 aacccgcggc tggtgatcgc gcgcatttcc gggtacgggc aggacgggcc ctatgcctcg
    11101 aagccgggct ttgcctcggt ctgcgaaggc atcagcggct tccgttacct gaacggcttt
    11161 cccggcgagg cgccggtacg gccgaacctg agcatcggtg acaccatcgc ggcgctgcac
    11221 gcggcgctcg gcatactgat cgcgctgcgt gcgcgcgatg cgctgcccgg cgccggcggt
    11281 caggtggtcg acgtggcgct gtacgaatcg atgttcaacc tgatggaagc cgtggtgccg
    11341 gagtactcgg gcgcggggtt gatccgcgaa ccggccggca ccacggtgac cggcatcgtg
    11401 cccaccaaca cgtaccgctg cgccgacggc aggttcgtgg tgatcggggg caacggcgat
    11461 tcgatcttca ggcggctgat ggaaggcatc ggccgccagg acctggccga
//
LOCUS       DAORUQ010000101        11310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_5947_length_11310_cov_5.773345, whole genome shotgun sequence.
ACCESSION   DAORUQ010000101 DAORUQ010000000
VERSION     DAORUQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5947_length_11310_cov_5.773345"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PLW13_09880"
     CDS             <1..1343
                     /locus_tag="PLW13_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70728.1"
                     /translation="AQFAIQCLMCHQQGSSTTRIARSEQDWHGIFDMMAEFGAVMTQQ
                     LYDEAPAVLNAAWDWNTRALDSFPDHPAVLDPAAAGVVIDEWAVGEQTSFLHDGVLGK
                     DGRLYAVDWIGDKLYRLDPRTNETKAWQVPTGELREGGILGILAERGRRYTHHTPTVA
                     PHSIQVAPDGKLWMTLSVGKGLASFDPVTEQFRTFDHPDRVKYPHTLRFDREGNIWYT
                     ASMTNHVVRFDTATESFEVFDLPTRNWSQWLMARAMGAIVWAANTFKVKGQSVVADPE
                     INPVPYGIDVAPDGRVWFSQFNNSRLGVITPATGAIEMIDTPFPAPRRFRIDSRGVLW
                     VPSYHEGKFFRFDPATRDFREYALPTGSGDMVYALAIDTRDDSIWLCGTNSDTLIHFD
                     PRTETFSTYLLPSRVSFTRELEVDAQGNVWTTTSNLPYYQMEGGQGKVIRLSFR"
     gene            1357..2169
                     /locus_tag="PLW13_09885"
     CDS             1357..2169
                     /locus_tag="PLW13_09885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70729.1"
                     /translation="MSSNGQGRLHGRVAVITGGASGIGAAAARLFVAEGARVVLGDLA
                     QGAGDALEQELNALAGAPVARFLRSDVTQLHEVEALVAAAERDFGRLDIMFNNAGIGS
                     FGDTVTLDVAQWKQVIDVDLNGVFHGCKAAIPALRRAGGGAIVNTASISGIGADYGFA
                     AYNAAKAAVINYTRTVALDHAAQNIRCNAVCPGWIDTPLTEATRGVEPLARAWREAIP
                     MRRAGNPAEIARVALFLASDEASYVTGTTVVADGGLTASNGQPNIPALLAGL"
     gene            2273..5725
                     /locus_tag="PLW13_09890"
     CDS             2273..5725
                     /locus_tag="PLW13_09890"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQY70730.1"
                     /translation="MLDPVLRQRLHHALASREAWWRRPRFLVPVGVLAAYALLGFLAL
                     PAWLRHALPDFARGQLGAQATVGELRINPFLATLEARDVALADAAGEPVLGFARLFID
                     FEFSSPLHRAWTFADLQLEQPVLDLQLARDGRLNLQRLLEALPPAEPAAAGEPARVLL
                     QRLAVVGGRLRYADLRGAEAVRAEFGPLSLQLDDIATIAGQRGQGSAQIGLPGGARLA
                     WSGELGLHPLASGGAVQLQGLEPRQLWDFARGRLRLAPPAGVIDASFDYEFATDGGAP
                     TLRADALRVGLRDLALQRSDDAAPLLALAAIELEGGSVDLAARSIGVESLRVNGGRVA
                     AAIDAAGVLDWSTLVPDAPGAAAPAPVASGAPARPWRLDLEAFALQDVALALADASRA
                     APLALDLAARSLGFGVSGVIGAGAPQLQLSGGALELADVALVAGAQREALARLASLSL
                     AGVRADLGARRVQAQRLAMHGLQTALERSADGTIRQLALLAATGDAGAVAVPESAARE
                     AAPAWSVALGELAVEGIEAALADASTQPALAYTVAGGKLVLRDIDTAAAAPLALEATL
                     PIAQGGRIGLGGSAAADGSAASLRLDVEQLALAPLAPLVTQRTVLALGSGSLFADAAL
                     DYRRGANGKPVLAVTGSAGVDGLLLTEAATGERFLAWRKLLATGIDLGLGPDRLSVRE
                     LRLTEPGAKFVVLEDRSTNLAQVLRASPPAVPAADVPEQAAAPQAAAAFPVTVERVRV
                     ENATVDFSDHSLVLPFAARIVQLNGSARGISPGAESRATLAFAGRVGEFGEAAIDGRL
                     APFDPARFTDIEVRFRNIAMNPLSPYSATFAGRTIERGDMDVALDYKMQDGILAGENR
                     IVLQDLQLGEKIESPGALDLPLDLAIALLSDSEGRIDVEVPVSGDVGDPQFSYGHVIG
                     QAIGKLITGVVTAPFRALGGLFGGAADSESLAVVRFTSGSAELMPRERERIARLADAL
                     AARPRLGVDVRPGVAPARDGYAVRRLIVRRAHAEELGLELASGEDPGAVAYDEAKSQR
                     ALEKLLEAREGEDAPAAFAAAYAREHGREPARVNPALALVGRASEDHEYYRALYLHLV
                     HGTPEPGAELQALARGRAEAVVAELAARGVEPPRLRIAAPAELAEARPSGVLLALELV
                     ARDG"
     gene            5843..7186
                     /locus_tag="PLW13_09895"
     CDS             5843..7186
                     /locus_tag="PLW13_09895"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQY70731.1"
                     /translation="MNPEASSRIVCKFGGSSLASAAQFRKVRAIVEADPRRRIVVPSA
                     PGKRDARDAKITDLLYLCHHAASIDAEFSSPFAQIVDRFVGIERELGLDAGIEAQLER
                     LREEFARGVTADYAASRGEHFCARLLAAYLGAEFVEAAECIVIGPQGIVDPESYALLG
                     ARLSDPGGRYVVPGFYGRDAEGHVRTFSRGGSDVSGAIAARAAHAALYENWTDVSGLL
                     MADPRIVESPRPMAEVTYAEIRELSYMGASVLHDEAMAPVREVGIPVNIRNTNDPAHP
                     GTRIVAKLSPEIEKNTLIAGVAGKTSFAMITIGKHLMNREVGFVYRLLGILEHHGVAF
                     EHCPSSIDSVSVVVDAGQMEGKAEDVLADIRRTLQPDELDYVPQVALIAIVGEGMSHA
                     IGIAAKVFAALAEARVNVRLINQGPSELNIIVGVAPEDYPMALRAIYAAFVAGAQ"
     gene            7265..9862
                     /locus_tag="PLW13_09900"
     CDS             7265..9862
                     /locus_tag="PLW13_09900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY70732.1"
                     /translation="MHRRRNLILNLAILVSIAATCAGLWAYFNRPVATPAWPENVWGY
                     SFSPFRAGQDPTRNVYPSEQEIAADIEMLAEQTNRLRTYSVRDSLGEIPRIAQEYGMN
                     VTLGVWISSDLVANEYEINRAIDIANRERNIDLVMVGNEAVYRGDVTPEQLSAYIDRV
                     RDNVKTLVTTAEPWHIWMKYPELADNVKVIGAHVLVYWEKEPSEYAVQGTIERAKLVR
                     KQFPKKRVLLAEVGWPSHGRAQGQAEAKPAEQAIYLRTLLNRLNAAGYEYFVIEAFDQ
                     PWKTGDEGDVGAYWGVYNEGRQQKFPFEGPVVAIPEWRSLAAVSIVLAVLAFGLLLID
                     GSGLKQRGRAFLALIAFGVASFLVFVAYDYSRQYLGLLEYVLGALLVLSAIGVFTVLF
                     TEAHELAETIWATRRRSFASVREPRGVLPKVSVHVPCYNEPPEMMIRTLDALARLDYP
                     HFEVLVIDNNTPDEETWRPVEAHCLALGPRFRFFHVRPLAGFKAGALNYALERTAPDA
                     EIVAVIDADYIVEPTWLQYLTPHFGNDPRIAVVQAPQDYRDGDESLFKKLCYAEYKGF
                     FHIGMVTRNDRDAIIQHGTMTMIRRDVLDRLRWGEWTITEDADLGLRVFAAGYSAAYV
                     VESHGRGVMPDRFIDYKKQRFRWAYGAMQIMKRHAGMLLLGRDTALTRGQRYHFLAGW
                     LPWCADGLNLFFTAGALVWTSAMLLVPKQALPPAMIFALPPLLLFFGKLAKILYVYLR
                     HMRVPVGTSIGAAVAGLALSHTIGKAVIYGLVTRTIPFFRTPKLAMRGGVMQALAEAR
                     EELYILILLWGAMAGLLVVHEMDTTDAYAWLAMLLLQSLAYLAAVVMSFLAVMPPRES
                     PLTLTQPAS"
     gene            9864..>11310
                     /locus_tag="PLW13_09905"
     CDS             9864..>11310
                     /locus_tag="PLW13_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucans synthase"
                     /protein_id="HQY70733.1"
                     /translation="MSRSRGAFVLLLVVAASVAFWWLLGREQHVPGALAGGQRLQCAS
                     YTPFTGTETPADFVAARRDQLERDFALLAPQVGCVRIYSVRGMEQIPEVAREHGMKVI
                     AGAWISRDDADTEREIAGLIALANRYPDVISAVLVGNEVLLRRERTAAQLLGYMQRVQ
                     AAVAQPVSYGDVWDFWLQNPSIVAGADFVTIHLLPYWENDPSDIGAAIAAVTHAHEQT
                     AKAFPGKDILIGETGWPSQGRQREGAVPGRVNQARFVRGFVAHADAEGWHYNLIEAFD
                     QPWKRGQEGAVGGYWGLFDATRHDKHILRGPVSNLPEWCECLGAALLLGAGLLLAGGG
                     WHAPGAMLVAALAANGVVFHLQQMRLFSRSASETGWFLLLAALAFALGWLGCRRLRGR
                     PAARWHEALIGIVAALAAIEMLGLAFDSRYRHFPLAVFAAPALVAWLFAPRAGPWRER
                     NRALGVLLVLCMPVVLWQETPLNLQALGWLAV"
BASE COUNT         1590 a         3912 c         4089 g         1719 t
ORIGIN      
        1 gcgcgcagtt cgccatccag tgcctgatgt gccaccagca gggttccagc accacgcgca
       61 tcgcgcgcag cgaacaggac tggcacggca tcttcgacat gatggcggag ttcggcgcgg
      121 tgatgacgca gcagctctac gacgaggcgc ccgcggtgct gaatgcggcc tgggactgga
      181 acacgcgcgc gctggacagc ttccccgacc atccggcggt gctcgatccc gcggcagcgg
      241 gggtcgtgat cgacgagtgg gcggtcggcg agcagacgtc cttcctgcac gacggcgtgc
      301 tcggcaagga cgggcgtctg tacgcggtgg actggatcgg tgacaagctc taccggctgg
      361 acccgcgcac gaatgaaacg aaggcctggc aggtgcccac cggggaactg cgcgaaggcg
      421 gcattctcgg gatactggcc gagcgcgggc gacgctacac ccaccacacg cccacggtgg
      481 ccccgcacag catccaggtg gcgcccgacg gcaagctgtg gatgacgctg agcgtgggca
      541 agggcctcgc cagcttcgat cccgtcaccg agcagttccg caccttcgat cacccggacc
      601 gcgtgaagta cccgcacacg ctgcgcttcg atcgtgaggg caacatctgg tacacggcga
      661 gcatgacgaa ccacgtggtg cgcttcgaca cggccacgga gtccttcgag gtgttcgacc
      721 tgcccacgcg caactggagc cagtggctga tggcgcgcgc gatgggcgcg atcgtctggg
      781 ccgccaacac cttcaaggtg aagggccagt ccgtggtcgc cgatcccgag atcaacccgg
      841 tgccctacgg catcgacgtc gcgcccgacg ggcgggtgtg gttctcgcag ttcaacaaca
      901 gccgcctcgg cgtgatcacg ccggccaccg gcgcgatcga gatgatcgac acgccgttcc
      961 cggcgccgcg gcgctttcgc atcgacagcc gcggcgtgct gtgggtgccc tcgtaccacg
     1021 agggcaagtt cttccgcttc gacccggcga cgcgcgactt ccgcgagtat gcgctgccca
     1081 cggggagcgg cgacatggtc tacgcgctcg cgatcgatac gcgcgacgat tccatctggc
     1141 tgtgcggcac caactcggac acgctgatcc atttcgatcc gcgcacggag acattcagca
     1201 cctacctgct gccgagccgc gtgagcttca cgcgcgagct cgaggtggac gcgcagggca
     1261 atgtctggac cacgacatcg aacctgccct actaccagat ggaaggcggg cagggcaaag
     1321 tgattcgact gagtttcagg tgagggaggc gcaggcatga gcagcaacgg gcagggcagg
     1381 ctccacggca gggtcgcggt gatcacgggc ggggcctcgg gtataggggc ggcggccgcg
     1441 cgcctgttcg tcgccgaggg ggcgcgcgtg gtgctgggcg atctcgcgca gggcgcgggt
     1501 gatgcgctcg agcaggaact gaacgcgctg gccggtgcgc ccgtggcgcg gttcctgcgc
     1561 agcgatgtca cgcagttgca cgaggtcgag gcgctggtgg ccgcggccga gcgcgacttc
     1621 ggccgcctcg acatcatgtt caacaacgcc ggcatcggca gcttcggcga caccgtcacg
     1681 ctcgatgtcg cgcagtggaa gcaggtgatc gacgtcgacc tcaacggcgt cttccacggc
     1741 tgcaaggcgg caattcccgc gttgcgccgc gccggcggcg gcgccatcgt caacaccgcc
     1801 tcgatctccg gcatcggtgc ggactacggc tttgccgcct acaacgccgc aaaggccgct
     1861 gtgatcaact acacgcgcac ggtggcgctc gatcacgccg cgcagaacat ccgctgcaac
     1921 gcggtctgcc cgggctggat cgacacgccg ctcacggagg ccacgagggg cgtcgagccc
     1981 ctcgccagag cctggcgcga ggcgatcccg atgcgccgtg ccggcaatcc cgcggagatc
     2041 gcccgggtcg cgctgttcct ggcgagcgat gaagccagct acgtcaccgg caccaccgtg
     2101 gtcgccgacg gtggcctcac ggcgagcaac ggccagccga acattcccgc gctgctggcg
     2161 ggcctctagg cggcgccgcg cgcccgcttt ctcgcggcgc gcgggcccgg ggtttcgcgg
     2221 cgccgggcat atcggctaac gttcggcgac gacccctgag ggagcgactc acgtgctcga
     2281 tccggttctg cgccagcgcc tgcaccatgc attggccagt cgcgaggcat ggtggcgccg
     2341 gccgcgtttc ctggtgccgg tcggtgtgct ggccgcctat gcgctgctcg gattcctggc
     2401 gcttccggcc tggctgcgcc acgcgctgcc ggatttcgcg cgcgggcagc tgggcgcgca
     2461 ggccacggta ggggaattgc gcatcaatcc cttcctcgcc acgctggagg cgcgcgacgt
     2521 cgcgctggcc gacgccgccg gcgagccggt gctcggtttc gcgcggctgt tcatcgactt
     2581 cgagttctcc agcccgctgc accgcgcctg gaccttcgcc gatctgcagc tcgaacagcc
     2641 cgtgctggac ctgcagctgg cgcgcgacgg gcgcctgaac ctgcagcgcc tgctggaggc
     2701 gttgccgccg gccgaaccgg cagccgcggg cgagccggcg cgcgtgctgc tgcagcgcct
     2761 cgcggtggtc ggcggacggc tgcgctacgc cgatctgcgc ggcgccgaag cggtgcgcgc
     2821 ggagttcggg ccgctctcgc tgcagctcga cgacatcgcc accatcgccg gacaacgggg
     2881 ccagggcagc gcgcagatcg ggctgcccgg cggcgcgcgg ctcgcgtgga gcggggagct
     2941 cggcctgcac ccgctcgcct cgggcggcgc ggtgcagctg cagggcctgg agccgcgcca
     3001 gctgtgggat ttcgcgcgcg ggcggctgcg gctggcaccg cccgcgggcg tgatcgacgc
     3061 atcgttcgac tacgagttcg cgaccgacgg cggggcgccg acactgcgtg ccgacgcgct
     3121 gcgcgtcggg ttgcgcgatc tcgccttgca gcgctccgat gacgcggccc cgctgctcgc
     3181 gctggcggcc atcgagctcg agggcgggag cgtggatctc gccgcgcgca gcatcggggt
     3241 ggagtcgctg cgcgtgaacg gcgggcgcgt cgcggccgcc atcgatgccg ccggcgtgct
     3301 cgactggtcc acgctggtgc ccgatgcgcc gggcgcggcg gcgcccgcgc cggtggcctc
     3361 gggcgcgccg gcgcggccct ggcgcctgga cctggaggcc ttcgcgctgc aggacgtggc
     3421 gctcgcgctg gccgatgcga gtcgtgccgc gccgctggcg ctcgatctcg ccgcccgcag
     3481 cctgggcttc ggtgtctccg gcgtgatcgg ggcgggtgcg ccgcaactgc agttgagtgg
     3541 cggcgcgctc gaactcgcgg atgtcgcgct cgtggccggt gcgcagcgcg aggcgctggc
     3601 tcgcctcgcg agcctctcgc tcgccggggt gcgggcggat ctcggggcgc gccgcgtgca
     3661 ggcgcagcga ctcgcgatgc acgggctgca gaccgcgctc gagcgcagtg ccgacggcac
     3721 catccgccag ctcgcgctgc tcgctgccac cggcgatgca ggcgcggtgg ccgttcccga
     3781 gtcggcagct cgcgaggcgg cgccggcgtg gagcgtcgcg ctcggcgagc tcgccgtcga
     3841 gggcatcgag gccgcgctgg ccgatgcgtc gacgcagccc gcgctcgcct acacggtcgc
     3901 tggcggaaag ctcgtgctgc gcgacatcga caccgccgcc gccgcgccgc tcgcgctcga
     3961 ggccacgctg ccgatcgcgc agggcgggcg catcggcctg ggcggcagcg cggccgcgga
     4021 tggcagcgcg gcctcgctgc ggctcgacgt cgaacagctg gcgctggcgc cgctcgcgcc
     4081 gctggtgacg cagcgcaccg tgctggcgct gggttccgga tcgctgttcg ccgatgctgc
     4141 cctcgactac cgccgtggcg ccaacggcaa gcccgtgctc gcagtgaccg gcagtgcggg
     4201 cgtggacggg ctgctgctca ccgaggcggc caccggtgag cgcttcctgg cgtggcgcaa
     4261 gctgctggcg accggcatcg atttgggcct cggtccggac cgcctgtcgg tgcgcgaact
     4321 gcgactgacg gagccgggcg cgaagttcgt cgtgctcgag gatcgcagca ccaacctcgc
     4381 gcaggtgctg cgcgcgtcgc cgccggcagt gcccgcggcg gatgtgcccg agcaggccgc
     4441 ggcaccgcag gccgcggccg cgttcccggt cacggtggaa cgcgtgcgcg tcgagaatgc
     4501 cacggtggac ttttcggatc acagcctggt gctgccgttc gcggcgcgca tcgtgcagct
     4561 gaacggctcg gcgcggggca tttccccggg cgccgagagc cgcgcgacgc tggccttcgc
     4621 cgggcgcgtg ggtgagttcg gagaggccgc gatcgacggc cggctcgcac ccttcgatcc
     4681 tgcgcgcttc acggacatcg aggtccgttt ccgcaatatc gcgatgaatc cgctctcgcc
     4741 ctacagcgcc accttcgccg ggcgcaccat cgagcgcggc gacatggacg tggcgctcga
     4801 ttacaagatg caggacggga tcctcgccgg cgagaaccgc atcgtgctgc aggatctgca
     4861 gctcggcgag aagatcgaga gccccggcgc gctcgacctg ccgctcgatc tcgcgatcgc
     4921 gctgctgagc gattccgagg ggcgaatcga cgtcgaggtg ccggtcagcg gcgatgtcgg
     4981 tgatccgcag ttcagctacg gccacgtgat cgggcaggcc atcggcaagc tcatcaccgg
     5041 cgtggtcacg gcgccgttcc gcgcgctcgg cggactgttc ggcggcgccg cggacagcga
     5101 atcccttgcc gtcgtgcggt tcacctcggg cagcgcggaa ctcatgccgc gcgagcgcga
     5161 gcgcatcgcg cggctggccg atgcgctcgc ggcacgcccg cgcctcggtg tggacgtgcg
     5221 gcccggcgtc gcgcctgcgc gcgacggcta cgcggtgcgg cgcctcatcg tgcgtcgcgc
     5281 gcacgccgag gagctcgggc tcgaactggc gtcgggcgaa gacccgggcg cggtcgccta
     5341 cgacgaggca aaaagccagc gcgcactgga aaagctgctc gaggcgcgcg agggcgagga
     5401 tgcacccgcg gcgtttgccg cggcttacgc acgcgagcac ggccgggagc ccgcgcgcgt
     5461 caatcccgcg ctggcactgg tggggcgcgc cagcgaggat cacgagtatt accgcgcgct
     5521 gtacctgcac ctggtgcacg gcacgccgga accgggcgcg gagctccagg cgctggcacg
     5581 cgggcgcgcc gaggcggtgg tggcggagct ggccgcgcgc ggcgttgagc cgccgcgcct
     5641 gcgcatcgcg gcgccggcag agctggccga ggcccggcca tccggcgtgc tgctggcgct
     5701 cgagctggtg gcgcgcgacg gatagcggca aaaggtgacc tgtttagttt cgctccggga
     5761 tgcgagaatg cgcgccgccc gggccggcgc cgccccgacg ccgcgcaccg gccgaatcgg
     5821 atcacccagg acggaaaacc tcatgaaccc ggaagcctcc tcccgcatcg tctgcaagtt
     5881 cggcggctcc agcctcgcca gcgccgcgca gttccgcaag gtgcgcgcca tcgtcgaggc
     5941 cgatccgcgg cgtcgcatcg tggtgccctc ggcgccgggc aagcgcgacg ccagggacgc
     6001 caagatcacc gacctgctgt acctgtgcca ccacgcggcc agcatcgatg ccgagttctc
     6061 ctcgccgttc gcgcagatcg tcgaccgctt tgtcggcatc gagcgcgagc tggggctcga
     6121 tgcgggcatc gaggcgcagc tcgagcgcct gcgcgaggag ttcgcccgcg gcgtcactgc
     6181 cgattacgcg gcttcgcgcg gcgagcactt ctgcgcccgg ctgctggcgg cctacctcgg
     6241 cgccgagttc gtcgaggcgg ccgagtgcat cgtgatcgga ccgcagggca tcgtggaccc
     6301 ggagagctac gcgctgctgg gcgcgcgcct gtccgatccc ggagggcgct acgtggtgcc
     6361 gggtttctac gggcgcgacg ccgagggcca tgtgcgcacc ttctcgcgcg gcggctcgga
     6421 cgtgagcggc gcgatcgccg cgcgggcggc ccatgccgcg ctctacgaga actggaccga
     6481 cgtctccggg ctgctgatgg ccgacccgcg catcgtcgag agcccgcggc cgatggccga
     6541 agtgacctat gccgagatcc gcgagctgtc gtacatgggc gcctcggtgc tgcacgacga
     6601 ggccatggcg ccggtgcgcg aagtgggcat cccggtcaac atccgcaaca ccaacgatcc
     6661 cgcgcatccc ggcacgcgca tcgtcgcgaa gctctcgccc gagatcgaga agaacacgct
     6721 gatcgccggc gtggcgggca agaccagctt cgcgatgatc acgatcggca agcacctgat
     6781 gaaccgcgag gtcggattcg tctatcgcct gctcggcatc ctggagcatc acggggtcgc
     6841 cttcgagcac tgcccgtcga gcatcgacag cgtcagcgtg gtggtggatg ccgggcagat
     6901 ggagggcaag gccgaggacg tgctggccga catccgccgc acgctgcagc ccgacgagct
     6961 ggattacgtg ccgcaggtcg cgctgatcgc gatcgtcggc gaaggcatgt cgcacgcgat
     7021 cggcatcgcc gcgaaggtct tcgccgcgct cgccgaggcg cgcgtgaacg tgcggctgat
     7081 caaccagggg ccctcggagc tcaacatcat cgtcggcgtg gcgccggagg attaccccat
     7141 ggcgttgcgc gccatctacg ccgcgtttgt cgcgggagcg caatgaatga tccgtgcgca
     7201 tgacgtccac ggtcccgtac ccgccgcggc gcgcggctga tctcccggag tggttcgcac
     7261 gcagatgcac agacgacgca acctgatcct gaacctcgcg atcctcgtga gcatcgcggc
     7321 gacctgcgcc gggctctggg cctatttcaa ccgcccggtg gccacgccgg cgtggcccga
     7381 gaacgtctgg ggctactcgt tctcgccgtt tcgcgccggg caggacccga cgcgcaacgt
     7441 ctatcccagc gagcaggaga tcgcggccga catcgagatg ctcgccgagc agaccaaccg
     7501 gctgcggacc tactcggtgc gcgacagcct gggcgaaatc ccgcggatcg cgcaggaata
     7561 cgggatgaac gtcacgctgg gcgtgtggat cagcagcgac ctcgtcgcca acgaatacga
     7621 gatcaaccgc gccatcgaca tcgccaaccg cgagcgcaac atcgatctcg tgatggtcgg
     7681 caacgaggcc gtgtatcgcg gcgacgtcac gccggagcag ctcagcgcct acatcgatcg
     7741 cgtgcgcgac aacgtgaaga ccctcgtcac cacggccgag ccctggcaca tctggatgaa
     7801 gtacccggag ctcgccgaca acgtcaaggt gatcggggcg cacgtgctgg tgtactggga
     7861 gaaggagccc tcggaatacg cggtgcaagg caccatcgag cgggccaagc tcgtgcgcaa
     7921 gcagttcccg aagaagcgcg tgctgctggc cgaggtgggc tggccctcgc acggtcgcgc
     7981 ccaggggcag gccgaggcga aaccggccga acaggcgatc tacctgcgca ccctgctgaa
     8041 ccggctgaat gccgccggct acgagtactt cgtgatcgag gccttcgacc agccgtggaa
     8101 gaccggcgac gagggcgacg tcggtgcgta ctggggcgtc tacaacgagg gccgccagca
     8161 gaagttcccg ttcgaggggc cggtggtggc gatccccgag tggcgttcgc tcgcggcggt
     8221 ctcgatcgtg ctcgcggtgc tggccttcgg cctgctgctg atcgacggct cggggctgaa
     8281 gcagcgcggg cgcgccttcc tcgcgttgat cgcctttggc gtggcctcgt tcctggtctt
     8341 cgtcgcctac gactactcgc gccagtacct gggtctgctg gagtacgtgc tgggcgcgct
     8401 gctggtgtta tcggcgatcg gcgtgttcac ggtgctgttc accgaggcgc acgagctcgc
     8461 cgagaccatc tgggcgacgc gccggcgctc gttcgcctcg gtgcgcgaac cgcgcggcgt
     8521 gctgcccaag gtgtcggtgc acgtgccctg ctacaacgag ccgccggaga tgatgatccg
     8581 cacgctcgat gcgctggcgc gactcgatta cccgcacttc gaggtgctgg tgatcgacaa
     8641 caacacgccc gacgaggaaa cctggcgccc ggtcgaggcg cactgcctgg cgctcgggcc
     8701 gcgctttcgc ttcttccacg tgcgcccgct cgccggcttc aaggcgggcg cgctgaacta
     8761 cgcgctggag cgcaccgcgc cggacgccga gatcgtcgcc gtgatcgacg ccgattacat
     8821 cgtcgagccg acctggctgc agtacctcac gccgcatttc ggcaacgatc cgcgcatcgc
     8881 cgtggtgcag gcgccgcagg actatcgcga cggcgacgag tcgctgttca agaaactctg
     8941 ctacgccgag tacaagggct ttttccacat cggcatggtc actcgcaacg accgcgacgc
     9001 catcatccag cacggcacga tgacgatgat ccgccgcgac gtgctggacc ggctgcgctg
     9061 gggcgagtgg accatcaccg aggacgccga cctcggcctg cgcgtgttcg ccgcgggcta
     9121 ttcggcggcc tacgtggtgg aatcgcacgg tcgcggcgtg atgcccgacc gcttcatcga
     9181 ctacaagaag cagcgtttcc gctgggccta tggcgcgatg cagatcatga agcgccacgc
     9241 cggcatgctg ctcctcgggc gcgacaccgc gctcacgcgc gggcagcgtt atcatttcct
     9301 cgccggctgg ctgccctggt gcgcggacgg gctgaacctg ttcttcacgg ccggcgcgct
     9361 ggtgtggacc tccgcgatgc tgctggtgcc caagcaggcg ctgccgccgg cgatgatctt
     9421 cgcgttgccg ccgctgctgc tgttcttcgg caagctggcg aagatcctgt acgtatacct
     9481 gcgccatatg cgcgtgccgg tcggcacctc gatcggtgcc gccgtggcgg gcctggcgct
     9541 gtcgcacacc atcggcaagg ccgtgatcta cgggctggtc acgcgcacca tcccgttctt
     9601 ccgcacgccg aagctggcca tgcgcggcgg cgtgatgcag gcgctggcgg aggcgcgcga
     9661 ggagctctac atcctgatcc tgctgtgggg cgcgatggcc gggctgctgg tggtgcacga
     9721 gatggacacc accgacgcct atgcctggct ggcgatgctg ctgctgcagt cgctggctta
     9781 cctggccgcg gtggtgatgt ccttcctcgc ggtgatgccg ccgcgcgaga gccccctcac
     9841 gctcacgcag ccggccagct gagatgtcgc gttcccgggg cgcgttcgtg ctgctgctcg
     9901 tcgtggcggc cagcgtggcg ttctggtggc tgctgggtcg cgagcagcac gtgcctggcg
     9961 cgctggccgg ggggcagcgc ctgcagtgcg cgtcgtacac gccgttcacc ggcaccgaga
    10021 cgccagccga tttcgtcgcc gccaggcgcg atcaactcga gcgggacttc gcgctgctcg
    10081 cgccgcaggt gggctgcgtg cgcatctact ccgtgcgcgg catggagcag atcccggagg
    10141 tcgcgcgcga gcacggcatg aaggtgatag cgggcgcctg gatcagccgt gacgatgccg
    10201 acaccgagcg cgagatcgcc ggcctgatcg cgctcgcgaa ccgttatccc gacgtcatca
    10261 gcgcggtgct ggtcggcaac gaggtgctgc tgcggcgcga gcgcaccgcg gcacagctgc
    10321 tcggctacat gcagcgcgtg caggccgccg tggcgcagcc cgtgagctac ggcgacgtgt
    10381 gggatttctg gctgcagaac ccgtccatcg tcgcgggcgc cgatttcgtt accatccacc
    10441 tgctgcctta ctgggagaac gaccccagcg acatcggtgc ggcgatcgcg gcggtgacgc
    10501 acgcgcacga gcagaccgcc aaggcattcc ccggcaagga catcctgatc ggcgagaccg
    10561 gctggccgtc gcagggacgc cagcgcgaag gcgccgtgcc ggggcgcgtg aaccaggcgc
    10621 gcttcgtgcg cggcttcgtg gcgcacgccg acgccgaggg ctggcactac aacctgatcg
    10681 aggccttcga ccagccgtgg aagcgcgggc aggaaggggc cgtcggcggt tactggggac
    10741 tgttcgacgc gacgcgccac gacaagcaca tcctgcgcgg accggtgtcg aacctgcccg
    10801 agtggtgcga gtgcctgggg gccgcgctgc tgctgggcgc cggattgctg ctcgccggcg
    10861 gcggctggca tgcgcccggc gcgatgctgg tcgcggcgct tgccgccaac ggtgtggtct
    10921 tccacctgca gcagatgcgc ctgttcagtc gcagcgcgag cgaaaccggc tggttcctgc
    10981 tgctggccgc gctcgcgttt gcgctcggct ggctgggttg ccggcgcctg cgcgggcgcc
    11041 cggcggcgcg ctggcacgag gcgctgatcg gcatagtggc ggcgctggcc gccatcgaga
    11101 tgctcgggct cgccttcgac tcgcgctatc gccacttccc gctggcggta ttcgccgcac
    11161 cggcgctcgt cgcctggctg ttcgcgccgc gcgccggccc gtggcgcgag cgcaaccgcg
    11221 cgctgggggt gttgctcgtg ctgtgcatgc cggtggtgct gtggcaggaa acgccgctga
    11281 acctgcaggc gctgggctgg ctggcggtga
//
LOCUS       DAORUQ010000102        11214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6005_length_11214_cov_5.704723, whole genome shotgun sequence.
ACCESSION   DAORUQ010000102 DAORUQ010000000
VERSION     DAORUQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6005_length_11214_cov_5.704723"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLW13_09910"
     CDS             complement(<1..347)
                     /locus_tag="PLW13_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70734.1"
                     /translation="MPGRPAGNGSIEEGLNIEDIPKRLLTLRALAALLATLALIQGVG
                     GLYRLADYRADRETALAATHWATAFGPPVWFTLLLVTLLGALSLMLSWRLPQSAPARW
                     LALFCALLAGGYGV"
     gene            complement(365..2470)
                     /locus_tag="PLW13_09915"
     CDS             complement(365..2470)
                     /locus_tag="PLW13_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70735.1"
                     /translation="MYPRLTSELRIGNMRVRNRIMLAAMGTELCEPDGCCGEPLLAYY
                     EARARGGTGLVMLETTSVSWPAGASMPRMVGISNEAHLPGLQAFTGRMHRHGAMAGVQ
                     LNHCGKVATEDRVQGRPLLVPSVPHFDKSDMFEALTRAEIASFVGGASQPVYQVMTQQ
                     DIDWLVGQFAAAAALAVRARFDCVEIHAGHGYVLSSFLSPHYNRRDDAYGGSAEKRAR
                     LLVEVLRAVRATVGPDFPILCRLDANEFRVSDGITAQDAVITARLAVEAGCNAIDVSA
                     YGNPTSGIAFTEAPIPHAPGAFLGFARAIRRAVDVPVIAVGRIEADAAERGIAAGHFD
                     MVAMGRKLLADPELPNKLAANDPLAIRPCIYCYVCVSQIFLNRHICCAVNASTGREAQ
                     DALIGPAERRKRVLVVGGGPAGLEAARVAAQRGHEVTLIEREPVLGGTARIAALAYEP
                     NARLVRHLEDAVARLPVIVCTRTTATADSIAALQPEAVLVATGARRIAPPIAGRELKH
                     VLDGEELRGLLLGGNAEAMRKLGLLQRAAVGAAQLTGITRNLDWMRALSRVYMPIGKR
                     VAIIGGGLVGLEVAELLAERGRELTVIEPGRDLGAELALVRRWRVLHTLDEHGVRLLR
                     GTTVAAIDANGLDCATRDGAERIAADTVIIALGTQPDTSLADALRARGIAAGTIGDCD
                     EIGYIQGAIDSGRAAALAL"
     gene            complement(2488..2937)
                     /locus_tag="PLW13_09920"
     CDS             complement(2488..2937)
                     /locus_tag="PLW13_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HQY70736.1"
                     /translation="MDDETLARALPGAWALERWEIRYSDGRAPSLPFGAEATGLLLYT
                     PQGYMSAGIARAARPRFDSPSTRHVPPERKCAAFDSYFHYQGRYRTRDGCVLHDVSDS
                     LNPDFPGSTQVRKATLDGDSLVLSAEDLLPGTTVKREHRLCWRRAGA"
     gene            3083..4420
                     /locus_tag="PLW13_09925"
     CDS             3083..4420
                     /locus_tag="PLW13_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70737.1"
                     /translation="MSIHQKSSAQTQEAVIFFAGGEIRREMLYPEFEALLDCVVPIRD
                     FAGKTLEAAYVRLDARLAPVTVVLFLIPFDSDGFPEQHWDVPLRMLAERGVRGPDLGG
                     GPVRLVTRGQCPLPQYEDQLWDIRFGAGGNTLAQLQERVLMNRLGLDVADQDLAQPPP
                     GAWPGAAAPAAPVPAPAAAPPAQGSEASDTLLALMQQSSQQMALAREQHQRESMALQQ
                     QLAVLQQHARALEDEKHALLARAEQQARTLEEQRSAADSRTRQLAEQARREIEAARQT
                     LEGERDAALAERDARMREALARLERERETQEDQLHSLRSELTELRRDKLRLMGEGADK
                     FFAQLKEKGVKLVAFQPGAGHLTIPMEDLNRYLEETEAFVAEKCGVSPEHYRRWLAHY
                     NSPLCQGTAGSGAPCAKPLTKLLKPAEFVAGMHDRCEIHKQFPRTQAAAEMPG"
     gene            4417..5355
                     /locus_tag="PLW13_09930"
     CDS             4417..5355
                     /locus_tag="PLW13_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQY70738.1"
                     /translation="MSRDRRHDFPELPAVETLRDARYAARGVTLDVLRLDRLHPLWGG
                     NKVFKLLPNLHRAEALGLRRVISFGGAYSNHLHALAAWGAEHGVQTVGIVRGEAPPRP
                     GPTLRELHERGMHIRHVSRADYRRRHDPAWVEEVTRALMPALVIPEGGDNAEGFAGCR
                     ELGRSIAALAGGWDVIALACGTGTTLAGVVAGSGAFVLGIAALRDAAGITARAAAHLA
                     PTAPRFHVLDRFAGRGFARIDAQLAAFMAQFTQRSGVPLDPVYTGKLFLGLHTLVGEG
                     RFPPGTRVLALHTGGLQGARGASPGRILPAAHAVPL"
     gene            5385..6272
                     /locus_tag="PLW13_09935"
     CDS             5385..6272
                     /locus_tag="PLW13_09935"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HQY70739.1"
                     /translation="MSQFRSVGLMGRVGSAAVLETLVHLRELLHARGLRVVVEREVAE
                     HLDPTPGEVRGREDIGRDTDLVIVVGGDGSLLGAARSLAPQNAPVLGVNRGRLGFLTD
                     ISPQQLEAQLDEVLAGRYLIESRFLLDVAVTRGDEVLARGDALNDVVLNSGISARMIE
                     FELYIEEQFVYTQRSDGLIISTPTGSTAYSLSGGGPIMHPGLDALVLVPMFPHTLGSR
                     PLVIHGSSSIRIVINEYNELHPTISCDGQLNITASPGDMIHVHKKPEHLRLLHPLDHN
                     FYDICRAKLGWGSRPDGDE"
     gene            6307..7188
                     /locus_tag="PLW13_09940"
     CDS             6307..7188
                     /locus_tag="PLW13_09940"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY70740.1"
                     /translation="MPAFKAIETRIGTELAQFSRYLWAQRVAHRIFDAPDGTQVLVVA
                     REQDVAPVREAFARWRAGELSLDDAAPAPRPAPGRIARAWRAQPVTLAGILLSVLGAA
                     IVMFDTRLEWLRWLAFTDFRALPGGRLDFESVAATYARGEYWRLLTPVFLHFGVLHLA
                     FNSLWLWELGRRIERARGHLTLLALLVLTGAGGNIAQYLFDGGVMFGGMSGVIYGLLG
                     YAWVWNRVSGAPGLVLPPGLMAVMLGWLLLCMSGLVEAIGFGAIANAAHGAGLALGAL
                     LGLGAALLYSGPPDPQP"
     gene            7203..7490
                     /locus_tag="PLW13_09945"
     CDS             7203..7490
                     /locus_tag="PLW13_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HQY70741.1"
                     /translation="MDFNTIVEMITPEVHASLRRAVEIGKWADGVALTREQREQCLQA
                     VIAWEARHLASEARTGYIDRGAKAEGELCATHPHDEEPAVIRIVRDSTGLG"
     gene            7490..8317
                     /locus_tag="PLW13_09950"
     CDS             7490..8317
                     /locus_tag="PLW13_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HQY70742.1"
                     /translation="MQELGSGALDKLRAALTAPVTYAMPVGESEVPLNEHLGRRLRIE
                     FLGLISCTHCGRRSKKSFGQGYCYPCFQKLARCDSCIVKPENCHFAQGTCREPEWGQQ
                     FCMTEHIVYLANSSGLKVGITRASQVPTRWIDQGAVQALPLLRVATRHQSGLAEILFG
                     ASVADKTNWRAMLKGPAEPLDLATEAAALLARHRNGLEDLQIRFGYDAIRTLDAATVP
                     IDYPVLEYPGKPQSLNLDKTPLIEGVLLGIKGQYLILDCGVLNVRNFTAYHVRLAAA"
     gene            8346..10997
                     /gene="pepN"
                     /locus_tag="PLW13_09955"
     CDS             8346..10997
                     /gene="pepN"
                     /locus_tag="PLW13_09955"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010793218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HQY70743.1"
                     /translation="MKDAQPRAILLSEYRVPAFLVSHVDLHVALGEETTRIRSRLTIA
                     RNPDGTEPGAALRLNGQALALVSLALDGAPLAAQDYSYDGEELCIAGVPDRFVLESEV
                     ELRPQDNTALEGLYRSRSMFCTQCEPEGFRRITFFPDRPDVLSLYSTTIVAERARFPV
                     LLSNGNRVDGGGLEDGRHWVRWEDPFPKPCYLFALVAGDLACVEDSFVTRSGRRVALR
                     IFVEAKDLDKCDFAMRSLQQAMRWDEEVYGREYDLDVFMIVAVDDFNMGAMENKGLNI
                     FNTSAVLANPEITTDASFLRIAAIVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDA
                     EFSADTGSRAVRRIENVNLLRTSQFAEDAGPMAHPVRPDSYIEINNFYTLTVYEKGAE
                     VVRMIRELIGSEAFRRGSDLYFARHDGQAVTTEDFVRAMEEASGRDLAQFRRWYTQAG
                     TPVLAVRGDYDAARRVYRLTVAQSCPATPGQPRKEPFHVPLRMGLVGAAGDLPLRLAG
                     DDSAPTERVLELTQPEHSFEFLDVAEPPIPSLLRQFSAPVKLRFDYSRDELAALMQRD
                     SDGVARWDAGQGLAVQVLEEQIAARRAGATKPLDPRLAAAMRVLLADTGSDAAMIAQM
                     LVLPGEAYLAELAETIEPLAIHHARMDARRALAAELRHELLACYERNRCDSAYRVEAE
                     DVARRSLKNACLAYLLLLDDAPCRELARRQYYAQDNMTDVLAALTAIVNSDSAAGRDA
                     ARELLADFHTRWRDEALAMNLWFQVQALRPGAGTLDDVKRLREHADFDVRNPNKVRAL
                     LGAFVHSNPGAFHRADHAGYRFLGEQVAAIDAMNPQVASRLLTPLTRWRRFEPVYAEG
                     MRTALKQLLERPGLSRDSFEVVSKSLA"
     gene            complement(11005..>11214)
                     /locus_tag="PLW13_09960"
     CDS             complement(11005..>11214)
                     /locus_tag="PLW13_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70744.1"
                     /translation="ASGLQVPMLFKQYTDAVTAGGAAFLDFGIDPDFGNCVDGLILVD
                     LSRLRDSHRARYLQPRMAAPESDRQ"
BASE COUNT         1624 a         3992 c         4006 g         1592 t
ORIGIN      
        1 acgccgtagc cgcccgcgag cagcgcgcag aagagcgcga gccagcgcgc gggcgcgctc
       61 tgcggcagcc tccagctcag catcagcgac agcgccccga gcagggtgac gagcaggagg
      121 gtgaaccaca cgggcgggcc gaaggcggtt gcccagtgcg tcgcggccag tgccgtctca
      181 cggtccgccc ggtaatcggc gagccggtag aggccaccga cgccctggat gagcgcgagc
      241 gtcgccagca gcgccgccag tgcgcgaagt gtcagcagtc gcttcggtat atcctcgatg
      301 ttcaggccct cctcgatgct gccgttccct gcggggcgcc ccggcaaccg caacccccct
      361 ccgctcagag cgcgagagcc gccgcgcggc cgctgtcgat ggcgccctgg atgtagccga
      421 tctcgtcgca gtcgccgatg gtgccggccg cgatgccgcg cgcgcgcagc gcatccgcga
      481 gcgaggtgtc gggctgcgtg cccagcgcga tgatcacggt gtccgccgcg atgcgctcgg
      541 cgccgtccct ggtggcgcaa tcgagcccgt tggcgtcgat ggccgcgacc gtggtgccgc
      601 gcagcaggcg caccccgtgt tcgtccagcg tgtgcagcac gcgccagcgc cgcaccaggg
      661 cgagttccgc gccgaggtcg cgtcccggct cgatcacggt gagctcgcgg ccgcgctcgg
      721 ccagcaactc cgcgacctcc agccccacca gcccgccgcc gatgatcgcc acccgcttgc
      781 cgatcggcat gtagacgcgg ctcagcgcgc gcatccagtc gagattgcgc gtgatgccgg
      841 tgagctgcgc ggcgccgacg gccgcgcgct gcaacaggcc gagcttgcgc atggcctccg
      901 cgttgccccc gagcagcagt ccgcgcagct cctcgccgtc gagcacgtgc ttcagttcgc
      961 gtcccgcgat gggcggggcg atgcgccggg cgccggtggc caccagcacc gcctcgggct
     1021 gcagcgcggc gatggaatcc gccgtggccg tggtgcgcgt gcagacgatc acgggcaggc
     1081 gcgccacggc gtcctcgagg tggcgcacga ggcgcgcatt gggttcgtag gcgagcgcgg
     1141 cgatgcgcgc ggtgccgccg agcacgggtt cgcgctcgat cagcgtcacc tcgtggccgc
     1201 gctgcgccgc gacgcgcgcc gcctcgagtc ccgcgggtcc gccgccgacc acgagcacgc
     1261 gcttgcgccg ctcggcgggc ccgatcaggg cgtcctgcgc ctcgcgcccg gtcgatgcgt
     1321 tcaccgcgca acagatatgg cggttcagga agatctggct gacgcagacg tagcagtaga
     1381 tgcagggacg gatcgccagc gggtcgttgg cggcgagctt gttcggcagc tccgggtcgg
     1441 cgagcagctt gcgtcccatc gcgaccatgt cgaagtgccc cgccgcgatg ccccgttccg
     1501 cggcgtcggc ttcgatgcgc ccgaccgcga tgacgggcac gtcgacggcg cggcggatcg
     1561 ctcgggcaaa gccgaggaag gcgcccggtg cgtgcgggat cggtgcctcg gtgaacgcga
     1621 tgccgctggt ggggttgccg taggcgctga catcgattgc attgcagccg gcctctaccg
     1681 ccaggcgcgc ggtgatcacg gcgtcctgcg ccgtgatgcc gtcgctcacg cggaattcgt
     1741 tggcatcgag gcggcagagg atcggaaagt ccggccccac cgtggcgcgc acggcgcgca
     1801 gcacctcgac cagcagccgt gcgcgctttt ccgcgctgcc gccgtaggcg tcgtcgcgcc
     1861 ggttgtagtg cggcgacagg aacgacgaca gcacgtagcc gtggccggcg tggatctcga
     1921 cgcagtcgaa gcgggcgcgc accgcgagcg cggccgccgc cgcgaactgc ccgaccagcc
     1981 agtcgatatc ctgctgcgtc atcacctggt acaccggctg cgaggcgccg ccgacgaagc
     2041 tggcgatctc ggcgcgggtg agggcctcga acatgtcgct cttgtcgaag tgcggcaccg
     2101 acggcaccag cagcgggcgg ccctgcacgc gatcctcggt cgcgaccttg ccgcagtggt
     2161 tcagctgcac gccggccatc gcgccgtggc ggtgcatgcg cccggtgaac gcctgcagtc
     2221 cgggcaggtg cgcctcattg gagataccca ccatgcgcgg catgctggcg cccgcgggcc
     2281 acgagaccga ggtggtctcg agcatgacca gtccggtgcc gccgcgcgca cgcgcttcgt
     2341 agtaggcgag cagcggttcg ccgcagcatc cgtcgggctc gcacagctcg gtgcccatgg
     2401 cggccagcat gatgcggttg cgcacgcgca tgttccctat gcgcagttcc gatgtgaggc
     2461 gtgggtacat ggcggacctc ccgcgtgtca ggcgccggcg cgccgccagc acagccgatg
     2521 ttcgcgcttc accgtggtgc cgggcaacag gtcctcggcc gagagcacca gggaatcgcc
     2581 gtccagcgtg gccttgcgca cctgcgtgct gcccgggaag tccgggttca gcgagtcgct
     2641 gacgtcgtgc agcacgcaac catcgcgggt gcggtagcga ccctggtagt ggaagtagct
     2701 gtcgaaggcg gcgcacttgc gttcgggcgg cacgtggcgc gtgctggggc tgtcgaagcg
     2761 cgggcgcgcg gcgcgcgcga tacccgccga catgtacccc tgcggggtgt acagcagcag
     2821 gccagtggcc tctgcgccga agggcaggct gggcgcgcga ccgtccgagt agcggatctc
     2881 ccagcgctcc agcgcccagg ctccgggcag ggcgcgggca agagtttcgt cgtccattgg
     2941 tctacgcttc ctggggcggc atgtggcgtg accctagcag gggggggccg cgggcggcaa
     3001 cccgggcggc ggctgttaac atgcggtgcg atccagcgac cgcggggttc cctcccgcgg
     3061 cggcgacgat acgggagtcc ggatgagcat tcaccagaaa tcctccgcgc agacgcagga
     3121 ggccgtgatc ttcttcgccg gcggggaaat ccgccgcgag atgctgtacc cggaattcga
     3181 ggccttgctc gactgcgtgg tgccgatccg cgatttcgcc ggcaagaccc tggaggccgc
     3241 ctatgtgcgc ctcgatgcgc gcctcgcccc ggtcacggtg gtgctgttcc tgattccctt
     3301 cgacagcgac ggcttcccgg agcagcactg ggacgtgccg ctgcgcatgc tggccgagcg
     3361 cggtgtgcgc ggacccgatc tcgggggcgg accggtgcgg ctggtgacgc gcggccagtg
     3421 cccgctgccc cagtacgagg atcagctctg ggacatccgc ttcggcgcgg gcggcaacac
     3481 cctggcgcag ctgcaggagc gggtgctgat gaaccgcctc gggctcgacg tggccgatca
     3541 ggacctggcc cagcccccgc ccggagcctg gcccggggcc gcggcgccgg cggctccggt
     3601 gccggcgcca gcggccgcgc cccccgcgca gggcagcgag gcctccgaca cgctgctggc
     3661 cctgatgcag cagagcagcc agcagatggc gctggcgcgc gaacagcacc agcgcgagag
     3721 catggcgttg cagcagcagc tggccgtgct gcagcagcat gcgcgggcgc tcgaggacga
     3781 gaagcacgcg ctgctggcgc gcgcggaaca gcaggcccgc acgctggagg agcagcgcag
     3841 cgctgccgac agcaggaccc gccagctggc cgagcaggcg cgccgcgaga tcgaggccgc
     3901 gcgccagacg ctcgagggcg agcgcgacgc cgcgctggcc gagcgcgatg cgcgcatgcg
     3961 cgaggcgctg gcgcgcctgg agcgcgagcg cgagacccag gaggaccagc tgcacagcct
     4021 gcgcagcgag ctcacggagc tgcgccgcga caagctgcgg ctgatgggcg agggcgcgga
     4081 caagttcttc gcccagctca aggagaaggg cgtcaagctg gtggccttcc agcccggtgc
     4141 cgggcacctg acgatcccga tggaagacct caaccgctac ctcgaggaaa ccgaagcctt
     4201 cgtggccgag aagtgcggcg tgagccccga acactaccgg cgctggctgg cgcactacaa
     4261 cagcccgctc tgccagggca ccgcgggcag cggcgcgccc tgcgccaagc cgctgaccaa
     4321 gctgctcaag cccgcggagt tcgtcgccgg catgcatgat cgctgcgaga tccacaagca
     4381 gtttccgcgc acgcaggcgg cagcggaaat gcccggttga gccgggaccg gcgtcacgac
     4441 tttcccgagc tgcctgccgt cgagacgctg cgcgatgcgc ggtacgctgc gcgcggcgtg
     4501 acgctcgacg tgctgcgcct cgataggctg cacccgctgt ggggcggcaa caaggtgttc
     4561 aagctgctgc cgaacctgca tcgggccgag gcgctgggac tgcggcgcgt gatcagcttc
     4621 ggcggcgcgt attcgaacca cctgcacgcg ctggcggcct ggggtgccga gcacggggtg
     4681 cagaccgtgg gcatcgtgcg cggcgaagcg ccaccgcggc ccggtcccac gctgcgcgag
     4741 ctgcacgagc ggggcatgca catccgccac gtctcgcgcg ccgattaccg gcgccgccac
     4801 gacccggcgt gggtggagga ggtgacgcgc gcgctgatgc cggcgctggt gatccccgag
     4861 ggcggcgaca acgccgaggg cttcgcgggc tgccgcgagc tggggcgctc gattgccgcg
     4921 ctggcgggcg ggtgggacgt gatcgcgctg gcctgcggca ccggcaccac gctggcgggc
     4981 gtcgtggccg gcagcggggc cttcgtgctc ggcatcgccg cgttgcgcga tgccgccggc
     5041 atcaccgccc gcgccgccgc ccacctggcc cccacggctc cccgcttcca cgtcctcgat
     5101 cgcttcgccg gccgcggttt cgcgcgcatc gacgcgcagc tggccgcgtt catggcgcaa
     5161 ttcacgcagc gcagcggcgt gccgctcgat ccggtctaca cgggcaagct gttcctcggc
     5221 ctgcacaccc tggtcggcga aggccgtttc ccgcccggca cgcgggtact ggcgctgcac
     5281 accggcgggc tgcagggtgc gcgcggcgca tcacccgggc gtatcctgcc tgcggcgcac
     5341 gccgtgccgc tataatccgc gcgctttcaa tggagccagc gagcatgagc caattccgca
     5401 gcgtcggact catgggccgg gtgggcagcg ccgcggtgct ggagaccctg gtccatctgc
     5461 gcgagctgct gcacgcgcgc ggcctgcgcg tggtagtgga acgcgaggtg gccgagcacc
     5521 ttgacccgac gcccggcgag gtgcgcgggc gcgaggacat cgggcgcgac accgatctgg
     5581 tgatcgtggt cggcggcgac ggcagcctgc tcggcgcggc gcgctcgctg gcgccgcaga
     5641 acgcgccggt gctcggcgtg aaccgcggcc ggctcggttt cctcaccgat atctcgcccc
     5701 agcagctcga ggcgcagctc gacgaggtcc tcgccgggcg ctacctgatc gaatcgcgct
     5761 tcctgctcga cgtggcggtg acgcgcggcg acgaggtgct ggcgcgcggc gacgcgctga
     5821 acgacgtggt gctgaactcc ggcatctcgg cgcgcatgat cgagttcgag ctctacatcg
     5881 aggagcagtt cgtctacacc cagcgctccg acgggctgat catctcgacg cccaccggca
     5941 gcacggcgta ttcgctctcg ggcggcgggc cgatcatgca ccccgggctc gatgccctcg
     6001 tgctggtgcc gatgttcccg cacacgctgg gcagccgccc gctggtgatc cacggcagca
     6061 gcagcatccg catcgtgatc aacgagtaca acgagctgca tccgaccatc agctgcgacg
     6121 gccagctgaa catcacggcc agccccggcg acatgatcca cgtccacaag aagcccgagc
     6181 acctcaggct gctgcacccg ctggaccaca acttctacga catctgccgc gccaagctcg
     6241 gctggggcag ccgtcccgat ggcgacgaat gaggccggca tgacgagcgc ggcagactga
     6301 atcgcgatgc cggcattcaa ggccatcgag acgcgcatcg gcaccgagct ggcgcagttc
     6361 agccgctacc tgtgggcgca gcgcgtggcg caccgcatct tcgacgcccc cgatggcacc
     6421 caggtgctgg tggtggcgcg cgagcaggat gtcgcgccgg tgcgcgaggc cttcgcgcgc
     6481 tggcgcgccg gcgaactcag cctcgatgac gccgcgccgg ccccgcgacc cgcgccgggc
     6541 cggatcgcgc gcgcctggcg cgcgcagccg gtcacgctcg ccggcatcct gctcagcgtg
     6601 ctgggtgcgg cgatcgtgat gttcgacacc cggctcgagt ggctgcgctg gctcgcgttc
     6661 acggatttcc gggcgctgcc cggcgggcgg ctcgacttcg agagcgtggc cgcgacctac
     6721 gcgcgcggcg agtactggcg cctgctgacg ccggtgttcc tgcacttcgg cgtgctgcac
     6781 ctggcgttca actcgctttg gctgtgggaa ctgggccggc gcatcgagcg cgcgcgcgga
     6841 cacctcacgc tgctcgcgct gctggtgctc accggggccg gcggcaacat cgcgcagtac
     6901 ctcttcgacg gcggcgtgat gttcggcggc atgtccggcg tgatctacgg gctgctcggc
     6961 tacgcctggg tgtggaaccg cgtttcgggc gcgcccgggc tggtgctgcc gccggggctc
     7021 atggccgtga tgctgggctg gctgctgctg tgcatgagcg gcctggtcga ggccatcggc
     7081 ttcggcgcga tcgccaacgc cgcgcacggc gcggggctcg cgctcggcgc gctgctcggg
     7141 ctgggggcgg ctttgttata ctcggggccc cccgatccgc agccctgagc agccacttca
     7201 tcatggattt caacaccatc gtggaaatga tcacccccga ggtgcatgcg agcctgcgcc
     7261 gcgccgtcga gatcggcaag tgggccgacg gtgtcgcgct cacgcgcgag cagcgcgagc
     7321 agtgcctgca ggccgtcatc gcctgggagg cgcgccacct cgcgagcgag gcgcgcaccg
     7381 gctacatcga ccgcggcgcc aaggccgagg gcgagctctg cgccacgcac ccgcacgacg
     7441 aggagcccgc ggtgatccgc atcgtgcgcg acagcacggg gctcggctga tgcaggagct
     7501 cggcagcggc gcgctcgaca agctgcgcgc cgcgctcacg gcgccggtca cctacgccat
     7561 gccggtgggc gagagcgagg tgccgctgaa cgaacacctc gggcggcgcc tgcgcatcga
     7621 gttcctcggc ctgatcagct gcacgcactg cgggcggcgc agcaagaaga gcttcggcca
     7681 gggctactgc tacccgtgct tccagaagct cgcgcgctgc gactcgtgca tcgtgaagcc
     7741 cgagaactgc cacttcgcgc agggcacctg ccgcgagccc gagtggggcc agcagttctg
     7801 catgaccgag cacatcgtgt acctggcgaa ttcctccggg ctcaaggtcg gtatcacgcg
     7861 cgcctcgcag gtgcccacgc gctggatcga ccagggcgcg gtgcaggcat tgccgctgct
     7921 gcgcgtggcg acgcgccacc agtcggggct ggccgagatc ctgttcggcg cctcggtggc
     7981 ggacaagacc aactggcgcg cgatgctgaa gggaccggcc gagccgctcg acctcgccac
     8041 cgaggccgcc gcgctgctcg cacgtcaccg caacgggctc gaggacctgc agatccgctt
     8101 cggctacgac gcgatccgca ccctggacgc cgcgaccgtg cccatcgact acccggtgct
     8161 cgaatacccc gggaagccgc agtcgctgaa cctcgacaag acgccgctga tcgagggcgt
     8221 gctgctcggc atcaagggcc agtacctgat cctcgattgc ggcgtgctga acgtgcgcaa
     8281 tttcaccgcc taccacgtgc gcctcgccgc cgcctgaggc cgcggccaac acggagtcat
     8341 ctgccatgaa agacgcgcag ccccgcgcca tcctgctcag cgaataccgc gtgcccgcct
     8401 tcctcgtgag ccacgtcgac ctgcacgtgg cgctcggcga ggagaccacg cgcatcagga
     8461 gccggctcac gatcgcgcgc aacccggacg gcacggagcc gggcgcggcg ctgcggctga
     8521 acggccaggc gctcgcgctg gtgtcgctcg cgctggacgg cgcgccgctc gccgcgcagg
     8581 actacagcta cgacggcgag gaactgtgca tcgccggcgt gcccgatcgc ttcgtgctgg
     8641 agagcgaggt cgagctgcgc ccgcaggaca acaccgcgct cgagggcctg taccgctcgc
     8701 gcagcatgtt ctgcacccag tgcgagccgg agggtttccg ccgcatcacg ttctttcccg
     8761 accgccccga cgtgctctcg ctctacagca ccacgatcgt cgccgagcgc gcgcgcttcc
     8821 cggtgctgtt gagcaacggc aaccgcgtcg atggcggcgg gttggaggac gggcgccact
     8881 gggtgcgctg ggaagacccg ttccccaagc cctgctacct gttcgcgctg gtcgcgggcg
     8941 atctcgcctg cgtcgaggac agcttcgtga cgcggtccgg acgccgggtg gcgctgcgca
     9001 tcttcgtcga ggccaaggat ctcgacaaat gcgatttcgc gatgcgctcg ctgcagcagg
     9061 cgatgcgctg ggacgaggag gtctacgggc gcgagtacga tctcgatgtc ttcatgatcg
     9121 tggcggtcga tgacttcaac atgggcgcga tggaaaacaa ggggctcaac atcttcaaca
     9181 cctcggcggt gctggcgaac ccggagatca ccaccgacgc ctcgttcctg cgcatcgccg
     9241 cgatcgtcgc ccacgagtac ttccacaact ggtcgggcaa ccgcgtgacc tgccgcgact
     9301 ggttccagct gagcctgaag gaaggcttca cggtgttccg cgacgcggag ttctccgccg
     9361 acaccggatc gcgcgcggtg cggcgcatcg agaacgtgaa cctgctgcgc accagccagt
     9421 tcgccgagga cgccggcccg atggcgcacc cggtgcgccc ggactcctac atcgagatca
     9481 acaacttcta cacgctcacg gtctacgaga agggcgccga ggtggtgcgc atgatccgcg
     9541 agctgatcgg cagcgaggcc ttccgccgcg gctcggacct gtacttcgcg cgccacgacg
     9601 gccaggccgt gaccacggag gatttcgtgc gcgccatgga agaagcgagc ggtcgcgatc
     9661 tcgcgcagtt ccgccgctgg tacacgcagg ccggcacgcc ggtgctggcg gtgcgcgggg
     9721 actacgacgc ggcgcggcgc gtctaccgcc tcacggtggc gcagagctgc ccggccacgc
     9781 cggggcagcc gcgcaaggaa ccgttccacg tcccgctgcg catgggactg gtgggtgctg
     9841 ccggcgacct gccgctgcgc ctggccggcg atgacagcgc gcccaccgag cgcgtgctcg
     9901 agctgacgca gcccgagcac agcttcgagt tcctcgacgt cgccgagcct ccgatcccca
     9961 gcctgctgcg ccagttctcg gccccggtga aactgcgctt cgactattcg cgcgacgagc
    10021 tcgcggcgct gatgcagcgc gacagcgacg gcgtggcgcg ctgggacgcg ggacagggac
    10081 tcgcggtgca ggtgctggag gagcagatcg cggcgcggcg tgccggcgcc acgaagccgc
    10141 tggatccgcg cctcgccgcg gcgatgcgcg tgctgctcgc cgacaccggc tcggacgccg
    10201 cgatgatcgc gcagatgctg gtgctgcccg gcgaggccta cctcgccgag ctcgccgaga
    10261 ccatcgagcc gctggcgatc caccacgcgc gcatggacgc gcgccgcgcg ctggccgccg
    10321 agctgcgcca cgagctgctg gcctgctacg agcgcaaccg ctgcgactcg gcctaccgcg
    10381 tcgaggccga agacgtcgcg cggcgcagcc tgaagaacgc ctgcctcgcc tacctgctgc
    10441 tgctcgacga cgcgccgtgc cgcgagctcg cccgccgcca gtactacgcg caggacaaca
    10501 tgaccgacgt gctggccgcg ctgacggcca tcgtcaacag cgacagcgcc gccgggcgcg
    10561 acgcggcgcg cgagctgctc gccgatttcc atacccgctg gcgcgacgag gcgctggcga
    10621 tgaacctgtg gttccaggtg caggcgctgc gcccaggcgc cggcacgctg gacgacgtga
    10681 aacgcctgcg cgagcacgcg gacttcgacg tgcgcaaccc gaacaaggtg cgcgcgctgc
    10741 tcggggcctt cgtgcattcg aaccccggcg ctttccaccg cgccgatcac gcgggttacc
    10801 gcttcctcgg cgagcaggtc gccgcgatcg atgcgatgaa tccgcaggtg gcctcgcgcc
    10861 tgctcacgcc gctgacgcgc tggcgccgct tcgagcccgt ttacgccgag ggcatgcgca
    10921 ccgcgctgaa gcaactgctc gaacgccccg gactctcgcg cgacagcttc gaggtggtgt
    10981 ccaagagcct ggcctgatcg ccgctcattg ccggtccgac tccggcgccg ccatcctggg
    11041 ctggaggtag cgcgcgcggt ggctatcgcg cagccgagac aggtcgacga ggatgagccc
    11101 gtcgacacag ttgccgaaat cgggatcgat accgaaatcg aggaaggcgg caccacccgc
    11161 ggttaccgcg tcggtgtact gcttgaacag catcggcacc tgcaggccgc tcgc
//
LOCUS       DAORUQ010000103        11068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6129_length_11068_cov_5.491056, whole genome shotgun sequence.
ACCESSION   DAORUQ010000103 DAORUQ010000000
VERSION     DAORUQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6129_length_11068_cov_5.491056"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1361)
                     /locus_tag="PLW13_09965"
     CDS             complement(195..1361)
                     /locus_tag="PLW13_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HQY70745.1"
                     /translation="MELKDKACIVGIGETAYCRKPGSGLSEESLQLHASVAALRDAGL
                     RGSQIDGVLAFPNVGKCEAFAASLGSENLHFAAIIHMGGAAPVASLRLAAMAVVSGAA
                     NHVLIPAGWNGYSGARVRQTVTTDKHSIPGGAIAWDYYLPQGLTVPPQWYALMARRHM
                     HEYGTTHEQLGAVALAMRRHAQLNPAALMHGKPLTMADYLASPMLADPYRVNDCCLET
                     DGAAACIVTTVERARDLGKPPVYIMGAAAGQPYPADEITNRKDFHRTGLSIAAPEAFR
                     MAGVTPADADFAQIYDCFTFEVIQQIEEAGFCRRGEGGPFVENGAIELGGRLPVNTHG
                     GLLSEAHSLGIGHIVEAVKQLRGSAGARQVADAEIGVVTGWGDFGDGSIAILRR"
     gene            complement(1383..2291)
                     /locus_tag="PLW13_09970"
     CDS             complement(1383..2291)
                     /locus_tag="PLW13_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY70746.1"
                     /translation="MSEPEATSSWSAWSSPGGEGLDNFAEILYEKKSHARLGGAVARV
                     TLNKPDKMNTLTLATVDEMFRAFYDANHDPMVGVIIVAAKGKHFGAGGDVAWERWGLR
                     EAFYNRYPHNRLMRMSRKPVIAQVQGYCLAGHNHMAYCCDFTIAADNAVFGQAGARVS
                     SPADGFFVPYLTKVVGAKKARELWMLCRKYPAAQALEMGLVNTVVPLDRLEAEVDQWC
                     EEMLALSPGCLEVLKAAFDQEMDGYRESCITSAAMFPDWFDMPEGKEGGAAFMEKRAP
                     EFWDIRRREAEARARLLEEYETRHEG"
     gene            complement(2334..3542)
                     /locus_tag="PLW13_09975"
     CDS             complement(2334..3542)
                     /locus_tag="PLW13_09975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY70747.1"
                     /translation="MARALEGIRVIDLTVWFQGPVSAQYLADFGAEVIKVERPQGGDQ
                     ARGVRSIKSLEIGDWNQYFLVNNRNKKSLVIDLRKPEGQQIMYRLVEKADVFLSNLTP
                     AMLDGWNLSWERLREINPRLVYATNTGYGRFGGASRPAFDMTVQALTGIMAQLGEPGQ
                     PPIYLGMGSGDAYGGIMSAFAIVTALYQRNRAGHGQCIDASLYGAQLFLAAPQLQSFL
                     AGNSERALQHSRREAANPLWNLYPTRGKWVYVCEPNEDARFRLLCEALGDGALIEDPR
                     FASAEGRAVNNVELIAEFDALAAALDHEALLAHCAALDIVAAPINTLADVVRDPQAWD
                     NDYLLKTWCEEVQREVEVRGLPVTLSKTPGEVNTLGLQLGQDTELLMMDLLEYSWDEI
                     EAFKAAGAIP"
     gene            complement(3558..4766)
                     /locus_tag="PLW13_09980"
     CDS             complement(3558..4766)
                     /locus_tag="PLW13_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY70748.1"
                     /translation="MSGALDDIVVLDVTRGFAPSMTAAFLGDFGARVIRLEPLGGASH
                     RRGVDEDGARSGTWNHEADLIHRNKQSLALDVATARGRAVLDDLLARCDVVLTDWPLA
                     ELAALGLDYAAACARRPDIVVARLSGFGPKGKDRELPAIDELAAARTGMMPIVQQPGE
                     PPVYTGAGAMHAAVLLAFGVVTALLHRQHSGEGQQVDTSLFAGNMYGASLDLQAFLAI
                     GSGDRLLNPISRLDVSNPMSGSLYASADGRWVTLTMPDTDRWWPDLAEMTGLDAGDPR
                     FDTHEKRCDLNRLELIHAFEAAFARQSAGHWRALFAARGMSADIIERFDYPASDPVAR
                     ANRYVVELDHPSFGRVKSLGFPIYMSESPARLASLAPCAGQHTLDVLHGLLGYPVETI
                     HELGACGVVA"
     gene            complement(4870..7302)
                     /locus_tag="PLW13_09985"
     CDS             complement(4870..7302)
                     /locus_tag="PLW13_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQY70749.1"
                     /translation="MSTRIPTSLDELPTTARRFVGKEVRRVEDRALVTGRAEFIDNLV
                     LPDMLHCAIQRSPHPHARIVRIDTSRAEAHPGVAAVLTGADLERWCNPMGTAPAGWGP
                     YSLAVGKTRFVGEPVAAVAASSRYIAEDACELIDVEYELLPVVADPCVATDPGSALLY
                     EENGTNLMLDRAFTWGDIDGVFAAADKVFSAKFRWNRVGANPTETFGCVCQWDLVQER
                     LTCHGAYQTPGFMALGRAHTLKLAANQINIISHPQGGGFGGKGGPRGTDIAALLSRKA
                     GGRPVKYIEDRMEYLLAGGGQSWDRYYDAALAVRADGTVTGFRVKLLDDQGASAEGYG
                     TISVAKPLAAFTGCYRIEAAQYDVKVVSTNRAPSYPYRGYGPPPHNFVLESLMDIAAR
                     GLGLDTAEMRRRNYIRPEQFPYTIPSGNEYDSGNYEATLDKVLEIADYGRLREEQARA
                     RAEGRLVGIGVASTIEPGVFDWNAYATVGVPGVGVPEGASVGINILGEVTVRVGFNLQ
                     GQGQYTLAAQLLADYFGVEMDAVRVVYADTDNAPPHFGPGGSRLGVAITGAILGACQA
                     ITEKLRHVAAVLLQAPFEQVELMDGMLRVRGVPEAQMPMAQVVGALLSRSDLLPPGME
                     ANASTTSVWTAPGRTAPDADGRCKSYLTAANACHVVLVEVDPETGKTEILKYFIADDC
                     GTRLNPATVEGMIQGGVAQGVGAALLEQYVYNENAQPLVSTFMDYLVPSINEVPMTEK
                     AALVTPSPFAPLGAKGCGEGAIHTTPAAIICAINDALAPLGAMVRETPASPHQVWSLI
                     RAARAASPHVPK"
     gene            complement(7328..7825)
                     /locus_tag="PLW13_09990"
     CDS             complement(7328..7825)
                     /locus_tag="PLW13_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY70750.1"
                     /translation="MTQQHKVRLSVNGRAYEREVEPRTTLVDFLRHELGLTGTHVGCE
                     HGVCGACTIIKDGKAVRSCLMLAVQADGAELQTVEGLHGENGLHPIQEAFIEKHGLQC
                     GFCTPGFLMTTVELLGDHPDPSDEDIREALGGNLCRCTGYQSIMESVRLAAAKLRAGA
                     AVQAD"
     gene            complement(7869..8750)
                     /locus_tag="PLW13_09995"
     CDS             complement(7869..8750)
                     /locus_tag="PLW13_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQY70751.1"
                     /translation="MKPVPFEYYAPETLQEAIALLAGFEEDGVEAKILAGGQSLMPML
                     SIRVARPDVLVDLRKLRLLDYIRDDGETIAIGAMATKRAAEDSSLIRERQPLFHAATL
                     NIGHRQIRSQGTVGGSFAHADPAAEYPAAAIALDMRMKAVGPQGERTIDARDFFVTYL
                     TTDLASSEILTEVCMPVLAAGTGWSFREVSRRKGDFALAGAAVLLSLAGGRIAGARIV
                     VFGVNATAVRLPSAEQLVLGQAPDDALFREAGLAGSVEVEEPLADVHASADFRRHLVS
                     VVVARGLAEALSRARPS"
     gene            8965..10071
                     /locus_tag="PLW13_10000"
     CDS             8965..10071
                     /locus_tag="PLW13_10000"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY70752.1"
                     /translation="MRNPLHTRLCDQLGIEYPIVAFTHCKDVAVAVINAGGFAVLGEA
                     MHPPEHIAADIRWIRERVHGKPFGIDLVLPASVPEEKSLEELYAMIPRAQREYTEMIK
                     QKYQVPDPKEKLEISNWGGLDQKRSLDQLEVLFDEKVPVIASGLGSPEFMLKRAHQLG
                     IQVWGLVGKPRQAKKQIAAGTDVIIAQGYDAAGHTGNIGTFSIVPQVVDAARSSGVPV
                     LAAGGVTTGRHLAAALALGADGVWTGSLWLASRESDVNLPMKEKLIESSADDTSYSDC
                     ISGYTMRTIRCPWHVEWSREDAPRALKPPLQMLLSNEYITGANDYLRKDLMTEAAGQG
                     IDYVTEMKPARQILSDMVEEALDVFDRFAADDEG"
     gene            10058..10348
                     /locus_tag="PLW13_10005"
     CDS             10058..10348
                     /locus_tag="PLW13_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70753.1"
                     /translation="MTRAESGDGAPFRAGFDAARAGRRAECDGGAAVAGTRFAGRQFF
                     AGTLTGDYRDFGDYPWRWYLMSSLSHAPQGYPHATVWCEAGSLEIEEDPGGE"
     gene            10429..>11068
                     /locus_tag="PLW13_10010"
     CDS             10429..>11068
                     /locus_tag="PLW13_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQY70754.1"
                     /translation="MRIVVCIKEVLDPSAVNNYALAGSLKIAADGRTLDVPGIPRLIN
                     AYDEQALEAALRLRDAGCECHITAVSIGRELQDPLKHCAALGADEIVSIDADPGALDH
                     HVVANILAAYIRSSGGADLVLCGRQASDDDQGVVPALLGESLDMPVAPIVRALTLQDG
                     RLRVTRVTPDGDEVLEGALPAVVTISNELGEPRFPSARDKMAARKKQALAVSV"
BASE COUNT         1731 a         3851 c         3733 g         1753 t
ORIGIN      
        1 cgaccaggcg aaggaattgc attgcgcgca gatctcgcgc ggcacgtgac ggaacgtccc
       61 gcagtccgcg cagcgctgga agcgcagctc gccctgccgg caccatgcgt agaacccggc
      121 cgtcaggcct tctagcgccg gcagcggctt gtcgtaggga atcgtttgcc tgctcatcgg
      181 gtgtcgctcc tccgctagcg ccgcaggatc gcgatgctgc cgtcgccgaa gtcgccccag
      241 ccggtgacca cgccgatctc ggcatcggcc acctggcgcg cgccggcgct gccgcgcagc
      301 tgcttcaccg cctcgacgat gtggccgatg ccgagcgagt gcgcctccga cagcaggccg
      361 ccgtgcgtgt tcaccggcag gcgcccgccg agctcgatcg cgccgttctc gacgaaggga
      421 ccgccctcgc cgcgccggca gaagccggct tcttcgatct gctggatcac ctcgaaggtg
      481 aagcagtcgt agatctgcgc gaaatccgca tccgcgggcg tcacgcccgc catgcggaac
      541 gcctcgggcg cggcgatgct caggccggtg cgatggaagt ccttgcgatt ggtgatctcg
      601 tcggccggat agggctgacc cgccgcggca cccatgatgt agaccggcgg cttgcccaga
      661 tcgcgcgcac gctccaccgt ggtgacgatg caggcggcgg cgccgtcggt ctccaggcag
      721 cagtcgttga cgcgataggg gtcggccagc atcggcgagg cgaggtagtc ggccatcgtg
      781 agcggcttgc cgtgcatcag ggccgcgggg ttcagctgcg cgtggcggcg catcgccagc
      841 gcgaccgcgc ccagctgctc gtgcgtggtg ccgtactcgt gcatgtggcg gcgcgccatc
      901 agcgcgtacc actgcggcgg caccgttagc ccctgcggca ggtagtagtc ccaggcgatc
      961 gcgccgccgg gaatcgaatg cttgtcggtg gtgacggtct ggcgcacgcg cgcccccgag
     1021 tagccattcc agccggcggg gatcagcacg tggttcgcgg cgcccgagac caccgccatc
     1081 gcggccaggc gcagtgaggc gaccggcgcc gcgccgccca tgtggatgat cgcggcgaaa
     1141 tgcaggtttt ccgagcccag gctcgcggcg aatgcctcgc acttgccgac gttcggaaag
     1201 gcgagcacgc cgtcgatctg gctgccacgc aggccggcat cgcgcagcgc cgcgaccgat
     1261 gcgtgcagtt gcagcgactc ctcgctcagc ccggagccgg gcttgcggca gtaggcggtc
     1321 tcgccgatgc cgacaatgca cgccttgtcc ttcaattcca tcgcagcttc cctcgacgcg
     1381 aatcagccct cgtggcgcgt ctcgtattcc tcgagcagcc gcgcgcgcgc ctcggcctcg
     1441 cgccggcgga tgtcccagaa ttcgggggcg cgcttttcca tgaacgcggc accgccttcc
     1501 ttgccctcgg gcatgtcgaa ccagtcgggg aacatcgccg ccgaggtgat gcacgactca
     1561 cgatagccgt ccatctcctg gtcgaaggcg gccttcagca cctcgaggca accggggctc
     1621 agcgccagca tctcctcgca ccactggtcc acttccgcct cgaggcgatc cagcggcacc
     1681 accgtgttga ccagccccat ctccagcgcc tgcgccgccg ggtatttgcg gcacagcatc
     1741 cacaactcgc gcgccttctt cgcacccacc accttggtca ggtagggcac gaagaaaccg
     1801 tcggcgggcg aggacacgcg cgcaccggcc tggccgaaca cggcgttgtc ggccgcgatc
     1861 gtgaaatcgc agcagtaggc catgtggttg tggccggcga ggcagtaacc ctgcacctgc
     1921 gcgatgacgg gcttgcgcga catgcgcatc aggcggttgt gcgggtaacg gttgtagaag
     1981 gcctcgcgca ggccccagcg ctcccaggcc acgtcgccgc cggcgccgaa gtgcttgcct
     2041 ttcgcggcca cgatgatcac cccgaccatc ggatcgtggt tggcgtcgta gaaggcgcgg
     2101 aacatctcgt cgacggtcgc cagcgtcagc gtgttcatct tgtcgggctt gttcagcgtc
     2161 acgcgcgcca ccgcgccacc caggcgcgcg tgcgacttct tctcgtagag gatctcggcg
     2221 aagttgtcga gcccctcgcc acccggcgag gaccacgcgc tccagctcga cgtcgcttcg
     2281 ggctcgctca tgtgtcaccg ccttccatga tcatggttgt cgccgtggcc ggctcagggg
     2341 atcgcgcccg cggccttgaa cgcctcgatc tcgtcccagg aatactcgag caggtccatc
     2401 atcaacagct cggtgtcctg gccgagctgc agacccagcg tgttcacttc acccggcgtc
     2461 ttcgacagcg tgaccggcag tccgcgcacc tcgacctcgc gctggacttc ctcgcaccag
     2521 gtcttaagca ggtagtcgtt gtcccaggcc tgcggatccc tgaccacgtc ggccagcgtg
     2581 ttgatcggcg cggcgacgat gtcgagcgcc gcgcaatgcg cgagcagcgc ctcgtgatcg
     2641 agtgccgcgg cgagcgcatc gaactccgcg atcaactcga cgttgttcac cgcgcgaccc
     2701 tcggcgctcg cgaagcgcgg atcctcgatg agcgccccgt cgccaagggc ctcgcacagc
     2761 aaccggaagc gcgcgtcctc gttcggctcg cagacgtata cccacttgcc gcgcgtcgga
     2821 tacaggttcc acagcgggtt cgccgcctcg cggcgcgagt gctgcagggc gcgctcgctg
     2881 ttgcccgcga ggaaggactg caattgcggc gcggcgagaa acagctgcgc accgtagagg
     2941 gaggcatcga tgcactgccc gtggcccgcg cggttgcgct ggtagagcgc cgtcacgatg
     3001 gcgaacgccg acatgatgcc gccgtaggca tcacccgagc ccatgccgag gtagatcggc
     3061 ggctgtcccg gctcgccgag ctgcgccatg atgccggtca acgcctgcac ggtcatgtcg
     3121 aaggcaggcc gcgacgcgcc gccgaagcgg ccatagccgg tgttggtcgc gtagaccagc
     3181 cgcggattga tctcgcgcag ccgctcccag gacaggttcc agccatcgag catcgcgggc
     3241 gtgaggttgg agaggaacac gtcggccttc tcgaccagcc ggtacatgat ctgctggccc
     3301 tcgggcttgc gcaggtcgat gaccaggctc ttcttgttgc ggttgttcac caggaagtac
     3361 tggttccagt cgccgatctc gagcgacttg atgctgcgca cgccgcgcgc ctggtcgccg
     3421 ccctgcgggc gttcgacctt gatcacctcg gcgccgaagt ccgcgagata ttgcgccgac
     3481 accgggccct ggaaccacac cgtcaggtcg atgacgcgga tgccctcgag tgctctggcc
     3541 accttgccct cccgcgctca ggcgacgacg ccgcaggcgc cgagttcgtg aatggtttcc
     3601 accgggtagc cgagcagccc gtggagcacg tcgagcgtgt gctgtcccgc gcagggcgcg
     3661 agactcgcga gccgcgccgg gctctcgctc atgtagatcg ggaatccgag gctcttcacg
     3721 cggccgaagc tcgggtgatc gagctcgacc acataacggt tggcacgcgc caccggatcg
     3781 ctcgccgggt aatcgaagcg ctcgatgata tccgccgaca tgcccctggc ggcgaacagc
     3841 gcgcgccagt gcccggcact ctggcgcgcg aacgctgcct cgaacgcatg gatcagttcc
     3901 aggcggttca gatcgcaacg cttctcgtgg gtgtcgaaac gcggatcccc ggcatccagc
     3961 ccggtcatct cggcgagatc cggccaccac cggtcagtgt cgggcatcgt gagcgtcacc
     4021 cagcgcccgt cggcgctggc gtagagcgag ccggacatcg ggttcgagac gtcgaggcgc
     4081 gagatcgggt tcagcagccg gtcgccggaa ccgatcgcga gaaaggcctg caggtcgagg
     4141 ctcgcgccgt acatattgcc ggcgaacagc gaggtatcga cctgctgccc ctcgccgctg
     4201 tgctggcgat gcagcagcgc ggtgacgacg ccgaaggcga gcagcacggc cgcgtgcatc
     4261 gcgcccgctc cggtgtagac cggcggctcg cccggctgct gcacgatcgg catcatgccg
     4321 gtgcgcgccg ccgcgagttc gtcgatcgcc gggagctcac ggtccttgcc cttgggcccg
     4381 aagccggaga gacgcgccac gacgatatcc gggcgccgcg cgcacgcagc ggcgtaatcg
     4441 agcccgagcg cggcgagctc cgcgagcggc caatcggtga gaaccacgtc gcagcgggcc
     4501 aggagatcgt cgagcaccgc acgcccgcgc gccgttgcca cgtcgagcgc gagactctgc
     4561 ttgttgcgat ggatcaggtc ggcctcgtgg ttccaggtgc cggagcgcgc gccgtcctcg
     4621 tcgacgccgc ggcgatgcga tgcacctccg agcggctcga gccggatgac gcgcgcgccg
     4681 aaatcgccga ggaacgcggc cgtcatcgag ggtgcgaagc cgcgcgtcac atcgagaacc
     4741 acgatatcgt ccagcgcacc actcatgccc gtcacccgtc gctgtgcatc accaagccga
     4801 tggtcctatc ctaatcgctg cgcccgttgc gacccgtttc gcagcgcatc aattccggcc
     4861 ttcgcgattt catttcggga catgggggct ggcagcccgc gcggcgcgga tcaacgacca
     4921 cacctggtgc ggcgaggccg gcgtctcgcg aaccatcgcg ccgagcggcg cgagcgcatc
     4981 gttgatggcg cagataatcg cggcgggcgt ggtgtggatc gcaccctcgc cgcagccctt
     5041 ggcgccgagc ggcgcgaacg gcgacggcgt caccagcgcc gccttctcgg tcatcggcac
     5101 ttcgttgatc gacggcacca ggtagtccat gaaagtcgat accagcggct gcgcgttctc
     5161 gttgtacacg tactgctcca gcagtgcggc gcccacgccc tgcgccacac ccccctggat
     5221 catgccctcg accgtcgcgg gattgaggcg cgtgccgcag tcatcggcga tgaagtactt
     5281 caggatctcg gtcttgccgg tttcggggtc cacctcgacc agcaccacgt ggcaggcatt
     5341 cgcggcggtg agatagctct tgcagcgtcc gtcggcgtcg ggtgcggtgc ggcccggcgc
     5401 ggtccagaca ctggtcgtgg aggcattcgc ctccatgccc ggcggcagca ggtcgctgcg
     5461 cgagagcagc gcgccgacga cctgcgccat cggcatctgc gcctcgggca cgcccctgac
     5521 gcggagcatc ccgtccatga gttcgacctg ctcgaacggc gcctgcagca gcaccgccgc
     5581 cacgtgacgc agcttttccg tgatcgcctg gcaggcgccg aggatcgcgc cggtgatcgc
     5641 cacgccgaga cggctgccgc cgggtccgaa gtgcggcggc gcgttgtcgg tgtcggcata
     5701 gaccacgcgc accgcgtcca tctccacgcc gaagtaatcg gccagcaatt gcgccgcgag
     5761 cgtgtactgg ccctgcccct gcaggttgaa gcccacgcgc acggtgacct cgccgaggat
     5821 gttgataccc accgacgcgc cctcgggcac gccgacgccg ggcacgccga ccgtggcata
     5881 ggcgttccag tcgaacaccc ccggctcgat ggtgctggcc acgccgatcc cgaccagacg
     5941 accctcggca cgcgcgcgcg cctgctcctc gcgcaagcgc ccgtaatcgg cgatctccag
     6001 caccttgtcg agcgttgcct cgtagttgcc gctgtcgtac tcgttgccgc tcggaatggt
     6061 gtaggggaac tgctccggac gaatgtagtt gcgccggcgc atctccgcgg tatcgaggcc
     6121 gaggccgcgc gcggcgatgt ccatcagcga ctcgagcacg aagttgtgcg gcggcggccc
     6181 gtagccgcgg taggggtagg aaggcgcgcg gttggtgctc accaccttca cgtcgtattg
     6241 cgccgcctcg atgcggtagc agccggtgaa ggccgccagc ggcttcgcga ccgatatcgt
     6301 gccataaccc tccgcgctcg cgccctggtc atcgagcagc ttcacgcgaa atccggtgac
     6361 cgtgccgtcg gctcgcaccg cgagcgccgc atcgtagtag cggtcccagg actggccgcc
     6421 gccggccagc aggtattcca tgcggtcctc gatgtacttc accggtcgcc cgccggcctt
     6481 gcgcgagagc agcgccgcga tgtcggtgcc gcgcggcccg cccttgccgc cgaatccccc
     6541 gccctgcgga tggctgatga tgttgatctg gttcgcggcc agcttcagcg tgtgcgcgcg
     6601 ccccagcgcc atgaagcccg gcgtctggta ggcgccgtgg caggtcaggc gctcctgcac
     6661 caggtcccac tggcacacgc agccgaaggt ttcggtcgga ttggcgccga cgcggttcca
     6721 gcggaacttg gcgctgaaca ccttgtcggc cgcggcgaag acaccgtcga tgtcgcccca
     6781 ggtgaaggcg cggtccagca tcaggttggt gccgttctcc tcgtagagca gcgcgcttcc
     6841 cgggtcggtc gccacgcagg ggtcggccac caccggcagc agctcgtact ccacgtcgat
     6901 cagctcgcag gcgtcttccg cgatgtagcg gctgctggcg gcgaccgcgg ccaccggctc
     6961 gccgacgaag cgcgtcttgc cgaccgcgag gctgtacggg ccccagcccg cgggcgcggt
     7021 gcccatcggg ttgcaccagc gctcgaggtc ggcgccggtg agcaccgcgg caacgcccgg
     7081 gtgcgcttcg gcccggctgg tatcgatgcg cacgatgcgc gcatgcggat gggggctgcg
     7141 ctggatcgcg cagtggagca tgtcgggcag cacgaggttg tcgatgaatt ccgcgcgtcc
     7201 cgtgaccagc gcgcgatcct cgacgcgccg gacctccttg cccacgaaac ggcgtgcggt
     7261 agtcggcaac tcgtccagcg aggtgggtat tctggtgctc atggcgtgtc ccttcccggt
     7321 ttgttgctca gtcggcctgc acggccgcac ccgcgcgcag cttcgcggcc gccagacgca
     7381 ccgattccat gatgctctgg tatccggtac agcggcacag gttgccgccc agcgcctcgc
     7441 ggatgtcctc gtcactcggg tccgggtgat cgccgagcaa ttccaccgtg gtcatcagga
     7501 accccggcgt gcagaagccg cactgcagcc cgtgcttctc gatgaaggct tcctggatgg
     7561 ggtgcaaccc gttttcaccg tgcagaccct cgacggtctg caactcggca ccgtcggcct
     7621 gcaccgcgag catgaggcag ctgcgcacgg ccttgccgtc cttgatgatc gtgcaggcgc
     7681 cgcacacacc gtgctcgcag ccgacgtggg tgcccgtgag gccgagctcg tggcgcagga
     7741 aatcgacgag cgtcgtgcgc ggttcgacct cgcgctcgta cgccctgccg ttcacgctga
     7801 gccggacctt gtgttgctgc gtcatggtca gttgcctcct tccgtgcaat cggtgcaata
     7861 cgcccgcgtc atgatggccg cgcgcgcgac aacgcctcgg ccagcccgcg cgccaccacc
     7921 accgacacga ggtggcggcg gaaatccgcc gaggcgtgca cgtcggcgag gggttcctcg
     7981 acctcgaccg acccggcgag cccggcctcc cggaacagcg cgtcatccgg cgcctgcccg
     8041 agcacgagtt gttccgcgga cggcagccgc accgccgtcg cgttgacgcc gaacaccacg
     8101 atgcgcgcgc cggcgatgcg cccgcccgcc agcgagagca gcaccgccgc gccggcaagc
     8161 gcgaagtcgc ccttgcgtcg cgagacctcg cggaacgacc agccggtgcc cgcggccagc
     8221 acgggcatgc acacctcggt caggatctcg ctcgaggcga gatccgtggt caggtaggtc
     8281 acgaagaaat cgcgcgcatc gatcgtgcgc tcgccctggg gtccgaccgc cttcatgcgc
     8341 atgtccagcg cgatcgcagc ggccggatat tccgcggccg gatcggcgtg cgcaaacgag
     8401 ccgccaaccg tgccctggct gcggatctgg cggtggccga tgttcagcgt cgccgcgtgg
     8461 aacagcggct ggcgctcgcg aatgagcgac gaatcctcgg cggcgcgctt ggtggccatc
     8521 gcgccgatgg cgatcgtctc gccgtcgtca cggatgtaat cgagcagccg cagcttgcgc
     8581 aggtcgacca gcacatcggg gcgcgcgacc cgtatgctca gcatcggcat caggctctgg
     8641 ccgccggcga ggatcttggc ttcgacgccg tcctcctcga agccggcgag caacgcaatg
     8701 gcctcctgca gggtttcggg tgcgtaatat tcgaaaggca ccggcttcat cgcgattacc
     8761 cgttgttggc tgtgacggct gtcttcgatc gcattctatg cgctgcgccc gtggcgccac
     8821 cagttgccgc gaatcaaatc aggccgatcg gatctcatat ccggtcaggt gcgcgatccg
     8881 gcgcaccagc cggtttcaat tgcgccggcg agcgattaga ttcgcttcgt cagagacgca
     8941 attccaacct ccgggaatac agacatgcgc aatccactgc acaccagact ctgcgaccag
     9001 ctcggcatcg agtacccgat cgtcgcattc acgcactgca aggacgtggc cgtggccgtc
     9061 atcaacgcgg gcggcttcgc ggtgctcggc gaggcgatgc atccgccgga gcacatagcc
     9121 gccgacatca ggtggatccg cgagcgcgtg cacggcaagc ccttcggcat cgacctggtg
     9181 ctgccggcct cggttcccga ggaaaagagc ctcgaggagc tctacgcgat gatccctcgc
     9241 gcgcaacgcg agtacaccga gatgatcaag cagaagtacc aggtcccgga tccgaaggag
     9301 aaactcgaga tctcgaactg gggcgggctc gaccagaagc gttcgctcga ccagctcgag
     9361 gtgctgttcg acgagaaggt gcccgtcatc gcctccggcc tgggttcgcc ggagttcatg
     9421 ctcaagcgcg cgcaccagct gggcatccag gtctggggcc tggtcggcaa gccgcgccag
     9481 gcgaaaaagc agatcgccgc cggcaccgac gtgatcatcg cccaaggcta cgacgccgcc
     9541 ggacacaccg gcaacatcgg cacgttctcg atcgtgccgc aggtggtcga tgccgcgcgc
     9601 agttccggtg tacccgtgct cgcggcgggc ggcgtgacca cgggacgtca cctggcggcg
     9661 gcgcttgcgc tgggagccga cggcgtgtgg acgggatcgc tgtggctcgc ctcgcgcgag
     9721 tccgacgtga acctgccgat gaaggagaag ctgatcgagt cgagcgcgga cgacacctcg
     9781 tactccgact gcatatcggg ttacacgatg cgcacgatcc gctgcccctg gcacgtggag
     9841 tggagccgcg aggatgcccc gcgcgcgctc aagccgccgc tgcagatgct gctgtcgaac
     9901 gagtacatca ccggggccaa cgattacctg cgcaaggatc tgatgaccga ggccgccgga
     9961 cagggcatcg actacgtcac ggagatgaag ccggcgcgcc agatcctctc cgacatggtc
    10021 gaggaggcgc tcgatgtctt cgaccgcttc gcggcagatg acgagggctg agagcggcga
    10081 cggcgcgccg tttcgcgccg gcttcgacgc cgcgcgagcg ggccggcgcg ccgagtgcga
    10141 cggcggggcc gccgtcgccg gaacgcgctt cgcgggtcgc cagttcttcg ccggcacgct
    10201 gaccggggac taccgggact tcggcgacta tccgtggcgc tggtacctga tgagctcgct
    10261 cagtcacgca ccgcagggtt acccgcacgc gaccgtgtgg tgcgaggcgg gcagcctcga
    10321 gatcgaggaa gaccccggcg gggaatagat tcgggagccg gctgcggccg gagggaagtt
    10381 tccggccgca accggaccgg agcaacgcag ggataacggg agatcgacat gcgaatcgtg
    10441 gtgtgcatca aggaagtgct ggaccccagt gccgtgaaca actatgcact cgccggcagc
    10501 ctgaagatcg cggccgacgg caggacgctc gacgtgccgg ggataccccg gctgatcaac
    10561 gcctacgacg agcaggcgct ggaggcggcg ctgcgactgc gcgacgccgg ctgcgaatgc
    10621 cacatcacgg cggtgtcgat agggcgcgaa ctgcaggatc cgctcaagca ctgcgcggcg
    10681 ctcggcgccg acgagatcgt ctccatcgac gcggatcccg gcgcgctcga tcatcacgtg
    10741 gtggcgaaca tcctggcggc ttatatccgc tccagcgggg gtgccgacct ggtcctttgc
    10801 ggccgccagg cctcggacga cgaccagggc gtggtgccgg cgctgctcgg cgagtcgctc
    10861 gacatgccgg tggcgcccat cgtgcgcgcg ctgacgctcc aggatggacg cctgcgtgtc
    10921 acgcgcgtga caccggacgg cgacgaagtg ctggaagggg cgctgcccgc cgtggtcacg
    10981 atcagcaacg aactcgggga gccccggttc cccagcgcgc gcgacaagat ggcggcacgc
    11041 aagaagcagg cgctcgcggt gtccgtcg
//
LOCUS       DAORUQ010000104        10951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6228_length_10951_cov_5.789097, whole genome shotgun sequence.
ACCESSION   DAORUQ010000104 DAORUQ010000000
VERSION     DAORUQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_10951_cov_5.789097"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLW13_10015"
     CDS             <1..314
                     /locus_tag="PLW13_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HQY70755.1"
                     /translation="WIDSSISKTINVPTDIPFEDFKDVYFYAYQNGLKGCTTFRFNPE
                     AFQGVLVKEKDLEKTTYQFKLEDGTTVELKGNEEVEYDGEMHTAANLFEAIKEGYYGK
                     F"
     gene            329..1021
                     /locus_tag="PLW13_10020"
     CDS             329..1021
                     /locus_tag="PLW13_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HQY70756.1"
                     /translation="MTVKIAKRIVGYKILDAVPEKPAVAEELERAIESMNENVSRPET
                     LPGATYKLKTPLSEHALYVTINDIVLNPGTAHEQRRPYEIFINSKNMDHFQWVLALTR
                     VVSAVFRKGGDCTFLVEELKAVFDPKGGYFKKGGRFMPSLVAEIGECIEKHLMDIGMI
                     QAPEIPEHAKRIMDEKRVQFEARSLNAAPAPEERNPYPAGAQLCNKCMTKAMIMMDGC
                     MTCLSCGDSKCG"
     gene            1177..2382
                     /locus_tag="PLW13_10025"
     CDS             1177..2382
                     /locus_tag="PLW13_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HQY70757.1"
                     /translation="MDLDAPVMERPGEAYTRQFRRDVETRSDQAQDEAMRSIDDVDVA
                     HLPQSPVAPFVMLEAEVAPGTSARLAWQPAESFDGIEVATPVLVVNGAAEGPVLCLTA
                     AVHGDELNGIEMVRRVLYDIDPQQLHGTVVGVPIVNLIGFKRASRYLSDRRDLNRHFP
                     GNPRGSSASRIAHSFFNQVISHCTILVDLHTGSFYRSNLPQIRGDLRRPEVVRVAEAF
                     TSTAIIHSPGAAGSLRRAAVERGIPAVTLEAGEPMRLQVDEVEHGVRGIFALIDQLGM
                     YRKLRVWGNPVAVHYQSRWVRSETGGILFGKAELGQQVAEGDLLGSVTDPITNMRADI
                     VSPYDGRVIGKAVDQVVLPGFAAFHIGIETSAEDLYEPRALVPAKDEEEDEERPDVDP
                     ESRDSLEDS"
     gene            2444..3400
                     /gene="tal"
                     /locus_tag="PLW13_10030"
     CDS             2444..3400
                     /gene="tal"
                     /locus_tag="PLW13_10030"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HQY70758.1"
                     /translation="MNQLEQLRAFTTVVADTGDIGSLAEFAPTDTTTNPSLLLQAAQK
                     PAYAHLLDDALGFGARVDAGVRDDAIMDKLAVNFGREILALIPGRVSTEVDARLSFDT
                     GATIARAERLIALYEDAGIGRERVLIKIASTWEGIRAAAELERRGIHCNLTLLFSFAQ
                     AAACAEAGVTLISPFVGRILDWYRKHEGRDAYPAAEDPGVRSVTRIYNYFKRNGYRTQ
                     IMGASFRNSDEIRALAGCDLLTIAPALMAELAAASAPLQRMLDPRTAMELGEAKAAPT
                     EAAFRWQMNEDAMATEKLAEGIRKFAEDTQKLQAFLHRRAGE"
     gene            3468..5600
                     /locus_tag="PLW13_10035"
     CDS             3468..5600
                     /locus_tag="PLW13_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HQY70759.1"
                     /translation="MPQLPASELPSRDPLLEALVYVARFHGKQISRESLTAGLPLHEN
                     RMVPSLVERAAERAGLETRIVECSPEALGGEAFPAILLLREDQACVLLGRDDGHLRVV
                     EAGGTETRITAQALGERYLGNAILARVAYGFDERAPELGGSAQRHWFWGAVLEHRALY
                     RDVLLAAAMISLFALALPLFTMNVYDRVVPNRATETLWMLAAGVVIVLGADFTLRSMR
                     GYFLDLASKRIDLKLSGYIMERVLGMRLEQRPASVGSFASNLRSFESARDFITSATVT
                     AFIDLPFALTFVIVIGWIAWPMLLPVLAAMVLVVGYAVTVGARMHELSETTYRAGAMR
                     NATLIESLVGLESVKALGAEGVMQSRWERSAAYLADRGAQLRLLANSTLNAAMLAQQL
                     ASVCTIVIGVYLISAGKLTMGGLIACSMLAARALAPMGQVAGLMTQYHNAITSLAALD
                     QIVENPVERPRGAHFLSRQSFRGDIEFRDVSFAYPGTDMNALRGVSLRINAGDKVAIL
                     GRTGSGKSTLLRLILGLYRPSSGAVLVDGIDLRQLDPGELRRNIGYVPQDVMLFYGTL
                     RENITIGAPQIEDAAIVRATQIGNIAEFVNQHPKGIDMPVGERGESLSGGQREGVAIA
                     RAVINDPPILLLDEPTGSMDHSGEEAVKAQLREFARERTVVIVTHRTSLLDMVDRIIV
                     TDNGRIVADGPKESVVEALRQGRVGRGV"
     gene            5597..7006
                     /locus_tag="PLW13_10040"
     CDS             5597..7006
                     /locus_tag="PLW13_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQY70760.1"
                     /translation="MSGSRIADTLRRMARRWIALSSPWLERVLERWNRPTPIDSVDWA
                     ADAHAARLARDPLRARTLLHVIVLVVLLLILWAALARIDEVTRGEAKVVPSRQLQVVQ
                     SLDGGIVSEIHAREGQVVDAGQLLVRIDATRFASALGENRVQRLSLLAKTARLAALAE
                     GKPFDVPPQVLAEAPELAAAETRLYQSSLAELEGQTGIARQQLAQRRQELQETTAFYQ
                     QSSRNYELAAQELSQTEPLLASGAVSEVEILRLKRDVSRLRGEREQSAAQMTRARAAI
                     DESERKVEQVTLDFRNAKRNELAEATATLNSLAEGSVGLSDRVKQAEVRSPVRGTIKR
                     LLVNTEGGVVLPGRDIVEIVPLDDTLLLEAKIAPRDIAFLSPGQHALVKFTAYDFVVY
                     GGLEAEVESIGADTITDDEGNAFYLVRVRTNKAMLRDKLPIIPGMVAEVDIMTGRKSV
                     LSYLLKPVLRAKQYALTER"
     gene            7003..7620
                     /locus_tag="PLW13_10045"
     CDS             7003..7620
                     /locus_tag="PLW13_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY70761.1"
                     /translation="MTQSLLITTDPEVRERWSGAFPGAPCLACAAEAQPWLDTDTIVW
                     LHLPHDDAAARELLWSSASRLAPARIVALSDTPSDEQALALMERGAVGYCHSHAGAAM
                     LRQVETVVSNHGLWVGPNLLQRLIASALSSDVRKPVVAGPLSTLSTREREVAMAVSRG
                     ASNKEIARELMITERTVKAHLSAIFLKLGVRDRLHLALMMRSQGT"
     gene            7677..8528
                     /locus_tag="PLW13_10050"
     CDS             7677..8528
                     /locus_tag="PLW13_10050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQY70762.1"
                     /translation="MARPLSEDWREWLRLNRERGCALGELYQSAASQGFSGDDISAEL
                     GGYRPAEPVEPVAPPHAASNDACGREEPWYSLFHAPLTRPEHKPRAWRLDTDLAQVYE
                     IPNLLTQEECEILMSVIDTSLQPSTVTRGPADYRTSRTCHMRRADPGLMKQIDARIAQ
                     LIGVDPAYSESIQGQRYDEGQYFKAHTDWFAPDTEEYEPHTNPGGQRTWTVMIWLNRV
                     DEGGATRFERIGRDFFPLPGAAVAWNNCDIDGKPNHNTLHEALPVIRGSKYVITKWFR
                     EKTGRND"
     gene            8541..9191
                     /locus_tag="PLW13_10055"
     CDS             8541..9191
                     /locus_tag="PLW13_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70763.1"
                     /translation="MSPVHPPRIAVCALRKIATPTPLQARILAEYARMNFAPVGGEAT
                     YDEEYGARQRGGISASGEAAPDIAYAPISGELFDACYETLTPMLEEWAGCPLTRSFGH
                     GIRSYGRGSILHLHRDRVDTHVISCIIHVDDRSDVPWPLDFIDHDGVLHQVTFTTGET
                     LFYESLCPHARLTPFQGDYYRNMYLHWRPTEWDPARYSGLKSKYASLQECLAQWGR"
     gene            9167..10348
                     /locus_tag="PLW13_10060"
     CDS             9167..10348
                     /locus_tag="PLW13_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70764.1"
                     /translation="MPRAMGSMRYLLYKDYASGLFNNWISFELGAGLAHLTKRRLVLY
                     GSVGEERRLLHIRGGNYQYLRPGFSAVLDNSRHPTVLDLMDTATVELLDYPAYRDAAQ
                     GLETLESDIKLTRAAYVHGARSAEDAMAQHGERLAEFLAGRDILVDPNEASWHLHACN
                     LGFYSRFFFNPTPELPSLLNNIRPRADYRNLAEKVSLALGPFNGVHIRLTDFKQFLPQ
                     GADYAGTILETLGSTFPRDELLVICTDESHNREFFAPITAAFPRHVFLDEFIVGEFAR
                     EFLGLPFTDEQSLGFVCNIVMWDAREFSGTPGSTYSGMIHRHWYSRRRAQDARSDPPF
                     RFINCGEAGKGGSEPGYFENGAFVECRKGHFSWTRTAIRRASETLSWYREWPECAIDS
                     R"
     gene            10469..>10951
                     /locus_tag="PLW13_10065"
     CDS             10469..>10951
                     /locus_tag="PLW13_10065"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HQY70765.1"
                     /translation="MPTGQPIATVVTLTGAAFARDAEGSLRRLRVGETVRQGEEIVTE
                     SGARVEIEFLSGGTLAIGESRTIAISPELAPEEAPDATESAVDSASVEQLLQSLGDSG
                     DLLDKLPATSAGFDGGVPGDGHDFVRVLRIVESVEPTPSPTPAPTPEPTPVPTPAPTP
                     A"
BASE COUNT         1968 a         3710 c         3524 g         1749 t
ORIGIN      
        1 agtggatcga ctcgtcgatc tccaagacca tcaacgtgcc gaccgacatc ccgttcgagg
       61 acttcaagga cgtctatttc tacgcctacc agaacggcct gaagggctgc accacgttcc
      121 gcttcaaccc ggaagccttc cagggtgtcc tggtcaagga aaaggacctc gagaagacga
      181 cctaccagtt caagctcgag gacggcacca ccgtagaact gaaaggcaac gaggaagtgg
      241 agtacgacgg cgagatgcac accgccgcga acctcttcga ggccatcaaa gaaggctact
      301 acggtaaatt ctgaggagta cccgcaacat gaccgtcaag attgccaagc gcatcgtggg
      361 ctacaagatc ctcgatgccg tgccggagaa accggccgta gccgaagagc tcgagcgcgc
      421 gatagagagc atgaacgaga acgtctcgcg ccccgagacg ctgcccggcg ccacctacaa
      481 gctcaagacg ccgctctcgg agcacgcgct gtacgtcacg atcaacgaca tcgtgctgaa
      541 cccgggcacc gcgcacgagc agcgccgccc ctacgagatc ttcatcaact cgaagaacat
      601 ggaccacttc cagtgggtgc tcgcgctcac gcgcgtggta tcggccgtgt tccgcaaggg
      661 cggtgactgc accttcctgg tcgaggagct gaaggcggta ttcgacccca agggcggcta
      721 cttcaagaaa ggcggccgct tcatgccctc gctggtcgcc gagatcggcg agtgcatcga
      781 gaagcacctg atggacatcg ggatgatcca ggcgccggag atccccgagc acgcgaagcg
      841 gatcatggac gagaagcgcg tgcagttcga ggcccgcagc ctcaacgcgg cccccgcccc
      901 cgaggagcgc aacccctacc ccgctggcgc gcagctgtgc aacaagtgca tgaccaaggc
      961 catgatcatg atggacggct gcatgacctg cctgagttgc ggcgactcga agtgcggctg
     1021 agcgcccccg ccgctgccac cgccggcctc gtgctggcac tggcagcggc ctgcttcgcc
     1081 cgcgccgcgg acgaggcact tcccgtacct gatgctcccg catcggcgac ggaaacgaca
     1141 gtttccgaca cgccgccgcg gcggcgcgca cgcaacgtgg acctcgacgc cccggtgatg
     1201 gagcgtccgg gcgaggccta cacgaggcaa ttccgccgcg acgtcgagac ccggtccgat
     1261 caggcccagg acgaggcgat gcgcagcatc gacgatgtcg acgtggcgca tctgccccag
     1321 agtcccgtcg ctcccttcgt gatgctcgag gccgaggtgg ccccgggcac ctcggcacgc
     1381 ctggcctggc agccagccga atccttcgat ggcatcgagg tggccacgcc ggtgctggtg
     1441 gtgaacgggg ccgccgaggg cccggtgctg tgcctgacgg cagcggtaca cggcgatgag
     1501 ctgaacggca tcgagatggt gcgccgcgtg ctctacgaca tcgatccgca gcagttgcac
     1561 ggcaccgtgg tcggggtgcc gatcgtgaat ctcatcggct tcaagcgtgc ctcgcgctac
     1621 ctgtcggacc gccgcgacct gaaccggcac ttcccgggca acccgcgcgg cagctcggcc
     1681 tcgcgcatcg cgcactcctt cttcaaccag gtcatttccc attgcacgat cctggtcgac
     1741 ctgcacaccg gatccttcta ccgcagcaac ctgccgcaga tacgcggtga cctgcgtcgc
     1801 ccggaggtgg tgcgcgtcgc cgaagcgttc acctcgaccg cgatcatcca cagccccggt
     1861 gccgcgggca gcctgcggcg cgcagcggtc gagcgcggca ttcccgccgt caccctggag
     1921 gcgggcgaac cgatgcgcct gcaggtcgat gaggtcgagc acggggtgcg cggcatcttc
     1981 gcgctgatcg accagctcgg catgtaccgc aagctgcgcg tctgggggaa cccggtagcg
     2041 gtgcactacc agtcgcgctg ggtacgctcc gaaaccggcg gcatcctgtt cggcaaggcc
     2101 gaactcggac agcaggtagc cgagggcgat ctgctcggca gcgtcaccga cccgatcacc
     2161 aacatgcgcg ccgatatcgt ctcgccctac gacggccgcg tgatcggcaa ggccgtcgac
     2221 caggtggtgc tgcccgggtt cgccgccttc cacatcggca tcgagaccag cgccgaggac
     2281 ctctacgagc cgcgcgccct ggtgccggcc aaggacgagg aggaggacga ggaacgcccc
     2341 gacgtggacc ccgagtcgcg tgacagcctc gaggattcgt gaaagcacgc gcaagctgca
     2401 ctatcctgcg tgaccggcag cacacgcaac aggttgagac ccgatgaacc agctcgaaca
     2461 actgcgcgcc ttcaccaccg tcgtcgccga taccggcgat atcggatcgc tggcggaatt
     2521 tgcacccacc gacaccacca ccaatccctc gctgctgctg caggcggcgc agaagcccgc
     2581 ctacgcccac ctgctggacg acgcgctggg cttcggcgcc cgcgtcgatg ccggcgtgcg
     2641 cgacgacgcc atcatggaca agctggcggt caatttcggc cgcgagatcc tggcgctgat
     2701 tcccgggcgc gtctccactg aagtcgatgc aagactgtcc ttcgacacgg gcgccacgat
     2761 cgcgcgcgcg gaacgcctga tcgcgctgta cgaggacgcc ggcatcggcc gcgagcgcgt
     2821 gctgatcaag atcgcctcga cctgggaggg catccgcgcc gcggcagagc tcgaacggcg
     2881 cggcattcac tgcaatctga ccctgctgtt cagcttcgcc caggccgcgg cctgcgccga
     2941 ggccggggtg accctcatat cgcccttcgt gggacgcata ctcgactggt accgcaagca
     3001 cgaggggcgc gatgcctacc ccgccgccga ggacccgggc gtgcgctccg tgacgcgcat
     3061 ctacaattat ttcaagcgca acggctatcg cacccagatc atgggcgcga gctttcgcaa
     3121 cagcgacgag atccgcgcac tggccggctg cgacctgctg accatcgcgc cggcgctgat
     3181 ggccgagctc gccgcggcaa gcgcgccact gcagcgcatg ctcgatccgc gcaccgcgat
     3241 ggagctcggc gaggcgaagg cggcgcccac cgaggcggcg ttccgctggc agatgaacga
     3301 ggacgcgatg gcgacggaaa agctcgccga gggcatccgc aagttcgccg aggacacgca
     3361 gaagctccag gccttcctgc atcgccgcgc gggcgaataa ggccgactat actgtgcagt
     3421 cgaccggcgc cgatgggtgc gccgacaccg cacggacacc agcgcagatg cctcaacttc
     3481 ccgcttccga gctgccctca cgggatcccc tgctcgaagc ccttgtctac gtggcgcgct
     3541 tccacggcaa gcagatctcg cgcgagagcc tgaccgccgg tttgcctctg cacgagaacc
     3601 gcatggtgcc ttcgctggtc gaacgcgccg ccgagcgcgc agggctcgag acgcgcatcg
     3661 tcgaatgcag cccggaggcg ctcggcggcg aggcgtttcc cgcgatactg ctgctgcgcg
     3721 aagaccaggc ctgcgtgctg ctcgggcgcg atgacgggca tctgcgcgtg gtcgaggcag
     3781 gcggaaccga gacccgcatc acggcgcaag cgctcgggga gcgctacctc ggcaacgcga
     3841 tcctcgcgcg cgtggcctac ggcttcgacg agcgcgcgcc cgaactcggc ggcagcgcgc
     3901 agcgtcactg gttctggggc gccgtgctgg agcatcgcgc gctgtaccgc gatgtgctgc
     3961 tcgccgccgc catgattagc ctgttcgcgc tggcgctgcc gctgttcacg atgaacgtct
     4021 acgaccgcgt ggtccccaac cgcgcgacgg agacactgtg gatgctcgcg gccggcgtcg
     4081 tcatcgtgct cggtgccgat ttcacgttgc gctcgatgcg cggctatttc cttgatctcg
     4141 ccagcaagcg gatcgatctg aagctttcgg gctacatcat ggagcgcgtg ctcggcatgc
     4201 gcctcgagca gcgcccggcc tcggtcggtt ccttcgcctc gaacctgcga tccttcgaat
     4261 cggcgcgcga cttcatcacc tccgccacgg tcacggcgtt catcgatctg ccctttgccc
     4321 tgacattcgt catcgtgatc ggctggatcg cgtggcctat gctgctgccg gtgctggccg
     4381 cgatggtgct cgtcgtggga tacgcggtca cggtcggggc gcgcatgcac gagttgtccg
     4441 agaccaccta ccgggccggc gccatgcgca atgccacgtt gatcgagagc ctggtgggac
     4501 tcgagtcggt caaggcgctc ggcgccgagg gcgtgatgca gagccgctgg gagcgcagcg
     4561 ccgcgtacct cgccgatcgc ggcgcgcagt tgcgcctgct cgcgaactcc acgctgaacg
     4621 ccgcgatgct cgcccagcag ctcgcctcgg tgtgcacgat cgtgatcggc gtctacctga
     4681 tatcggctgg caagctcacg atgggcgggc tcatcgcgtg ctcgatgctg gccgcgcgcg
     4741 cactggcacc gatgggacag gtggcgggcc tgatgacgca gtaccacaac gcgatcacct
     4801 cgctggcggc actcgaccag atcgtggaaa acccggtcga gcgcccgcgc ggtgcccact
     4861 ttctcagtcg ccaatccttt cgcggcgaca tcgagtttcg cgacgtcagc ttcgcctacc
     4921 ccggaaccga catgaatgcg ctgcgcggcg tgagcctgcg catcaacgcc ggcgacaagg
     4981 ttgcgattct cggtcgcacc ggatcgggca agagcacgct gctgcgactc atcctcggtc
     5041 tctaccgccc gagctccggt gcggtgctgg tcgacggcat cgatctgcgc cagctggacc
     5101 ccggcgagtt gcgccgcaac atcggctacg tgccgcagga tgtcatgctc ttctacggca
     5161 cgctgcgcga gaacatcacc atcggggcgc cgcagatcga ggacgccgcg atcgtgcgcg
     5221 ccacgcagat cggcaacatc gccgagttcg tgaaccagca tccgaagggc atcgacatgc
     5281 ccgtcggcga gcgtggcgaa tcgctatccg gcggccagcg cgagggtgtc gcgatcgcgc
     5341 gcgcggtgat caacgatccg ccaatcctgc tgctcgacga acccaccggt tccatggacc
     5401 actcgggcga ggaagcggtg aaggcgcaac tgcgagaatt cgcgcgcgag cgcaccgtgg
     5461 tgatcgtcac ccaccgcacc tcgctgctcg acatggtcga tcgcatcatc gtcaccgaca
     5521 acggccggat cgtcgctgac ggcccgaagg agagcgtggt cgaggcactg cgccaggggc
     5581 gcgtcgggag gggcgtatga gcggatcgcg catcgccgat acgctcaggc gcatggcgcg
     5641 gcgctggatc gccctcagct caccgtggct cgagcgggtg ctcgagcgct ggaaccgccc
     5701 cacccccatc gacagcgtcg actgggccgc cgatgcacac gccgcgcgcc tcgcgcgcga
     5761 tcccctgcgc gcacgcacgc tgctgcacgt catcgtactg gtggtgctgc tgctcatcct
     5821 gtgggccgcc cttgcgcgca tcgacgaggt cacgcggggc gaggccaagg tggtcccttc
     5881 acgccagctg caggtcgtgc agtcgctcga tggcggcatt gtttccgaga tccacgcgcg
     5941 cgaaggacag gtcgtcgatg ccggccaact gctggtgcgc atcgacgcca cgcgcttcgc
     6001 ctcggcgctc ggcgaaaacc gcgtgcagcg cctgtcactg ctcgccaaga ccgcacgcct
     6061 ggcggcgctt gccgagggca agccctttga cgtgccgccg caggtgctcg ccgaggcgcc
     6121 cgagctcgcc gcggccgaaa cccgcctgta ccagtcgagc ctcgccgagc tggaaggcca
     6181 gaccggcatc gccaggcagc agctcgccca gcgccgccag gaattgcagg agaccacggc
     6241 gttctaccag cagtcgtcgc gaaactacga actggccgcg caggaactca gccagaccga
     6301 gcccctgctc gcgtccggcg cggtgtcgga ggtggaaatc ctgcgtctca agcgcgacgt
     6361 ctcgcgcctg cgtggcgagc gcgagcagtc cgccgcgcag atgacccgcg ccagggcggc
     6421 tatcgatgag tccgaacgca aggtcgagca agtcacgctc gactttcgca acgccaagcg
     6481 caacgaactc gcggaagcca ccgcaacgct caacagcctt gcagagggca gcgtcggcct
     6541 gtccgaccgc gtgaagcagg ccgaggtccg ttcgccggtg cgcggcacca tcaagcggct
     6601 gctggtcaac accgagggtg gcgtggtgct gccggggcgc gacatcgtcg agatcgtgcc
     6661 gctcgatgac acgctgctgc tggaagcgaa aatcgcgccg cgcgacattg cctttctcag
     6721 ccccggccag catgcgctgg tgaaattcac cgcctacgat ttcgtggtct acggcgggct
     6781 cgaggccgag gtcgagagca tcggcgccga cacgatcacc gacgatgaag gcaacgcgtt
     6841 ctacctggtg cgcgtgcgca cgaacaaggc gatgctgcgc gacaagctgc cgatcattcc
     6901 cggcatggtg gccgaagtgg acatcatgac gggccggaaa agcgtgctaa gctacctgct
     6961 gaaacccgtc ttgcgcgcca agcagtacgc actcaccgaa cgatgacgca gagccttctg
     7021 atcacgaccg accccgaggt gcgggagcgc tggtccgggg ctttccccgg ggcaccgtgc
     7081 ctggcttgtg ctgctgaagc ccagccctgg ctggacaccg acaccattgt ctggttgcac
     7141 ctgccgcacg acgacgccgc tgccagggag ttgctgtgga gcagcgcctc gcggctcgcg
     7201 ccggcgcgca tcgtggcgct gtccgacacc ccctccgacg agcaggcact cgcattgatg
     7261 gaacgcggag ccgtgggcta ctgccactcg cacgcgggcg ctgcgatgct gcgacaggtc
     7321 gagacggtgg tcagcaatca cggcttgtgg gtcggcccca acctgctgca acggctcata
     7381 gcctctgcgc tgtcatcgga tgtgcgcaaa cccgtggtcg cgggaccgct cagcacgctg
     7441 tcgacacgcg agcgcgaggt ggcaatggcg gtgagccgcg gcgctagcaa caaggaaatc
     7501 gcgcgcgaac tgatgatcac ggagcgcacg gtcaaggcgc acctgagcgc gatcttcctc
     7561 aagctcggcg tgcgcgaccg gttgcacctg gcgctgatga tgcgctccca gggcacctga
     7621 gattccgctg taccgggcaa gagagacctg aggacaccat gcgaggacaa ggcactatgg
     7681 cgcgtccgct ttccgaggac tggagggaat ggctgcgcct gaaccgcgaa cgcggctgcg
     7741 cactgggtga actgtatcag agcgcagcga gtcagggctt ttccggggac gacatctccg
     7801 ccgaattggg tggctaccgt cccgccgagc cggtggaacc ggtggcaccg ccgcatgcag
     7861 catccaacga cgcctgcggg cgcgaggaac cgtggtacag cctcttccac gcgccgctga
     7921 cgcgcccgga gcacaaaccg agggcgtggc gactggatac cgacctcgcc caggtctacg
     7981 agattcccaa cctgctgacg caggaggaat gcgaaattct gatgagcgtc atcgacacct
     8041 cgctgcagcc ttcaacggtc acgcgcggac cggcggatta ccgcaccagc cgcacgtgcc
     8101 acatgcgccg ggcggatccc gggctgatga agcagatcga tgcgcgaatc gcgcaattga
     8161 tcggcgtcga tccggcctac tccgaatcga tccagggcca gcgctacgac gaagggcagt
     8221 acttcaaggc gcacaccgat tggttcgcac cagacaccga ggaatacgag ccgcacacga
     8281 atccgggagg gcagcgcacc tggaccgtga tgatctggct gaaccgtgtg gatgaaggcg
     8341 gcgcgacgcg cttcgagcgc atcgggcgcg atttctttcc gctgcccggc gccgccgtgg
     8401 cgtggaacaa ctgcgacatc gacggcaagc ccaaccacaa taccctgcac gaggcactgc
     8461 ctgtgatcag gggcagcaag tacgtgatca ccaagtggtt ccgggaaaag accggccgca
     8521 acgactgacg gaacagattc atgtccccag tgcatccacc tcgtatcgcg gtctgcgcgt
     8581 tgcgcaagat cgcgaccccc acccccttgc aggcccgcat ccttgccgaa tacgcccgca
     8641 tgaatttcgc gcctgtcggc ggtgaggcca cctatgacga ggagtacggc gcgcgccagc
     8701 gcggcggaat atcggcctcc ggcgaagcag cgcccgatat cgcctacgcg ccgatcagcg
     8761 gcgaactgtt cgatgcctgc tacgagacct tgacgcccat gctcgaggaa tgggccggtt
     8821 gtccgctcac gcgcagtttc ggccacggga tacgcagcta cgggcgcggg tcgatcctgc
     8881 acctgcaccg cgaccgcgtg gacacgcacg tgatcagctg catcatccat gtcgatgatc
     8941 gctcggatgt gccgtggccg ctcgacttca tcgatcacga cggcgtgctg catcaggtca
     9001 cgttcaccac cggagagacg ctgttctacg agagcctctg cccgcacgcc cgtctcacac
     9061 cgttccaggg cgactactac cgcaacatgt atctgcactg gcggcccacc gagtgggatc
     9121 cggcgcgtta cagcggcctg aaatccaagt acgcatcgct gcaggaatgc ctcgcgcaat
     9181 ggggtcgatg aggtatctgc tttacaagga ttacgcctcc ggtctgttca acaactggat
     9241 ctccttcgag ctcggggcgg gacttgctca cctgacgaag cgtcgcctgg tgctttacgg
     9301 ctcggtcgga gaggaacgaa gactgctcca catccgcggc ggcaactacc agtacctgcg
     9361 gccgggtttc agcgccgtgc tcgacaacag caggcatccc acggtgttgg acctgatgga
     9421 caccgcgacc gtggaattgc tcgactaccc tgcgtatcgc gatgccgcgc agggcctgga
     9481 gacgctggaa agcgacatca agctgacgcg ggcggcctac gtgcacggcg cgcggagcgc
     9541 cgaagatgcg atggcgcaac acggggagcg cctggcggaa ttccttgccg gccgtgacat
     9601 cctggttgac cccaacgagg cctcgtggca cctgcacgcc tgcaatctcg gcttttactc
     9661 gcgattcttt ttcaacccga ccccggaact tccgtcgctg ctgaacaata tccgaccgcg
     9721 cgcggattac cgcaatctcg cagagaaggt ttcgctcgcg ctggggccgt tcaacggcgt
     9781 acacatcagg ctgacagact tcaaacagtt cctgccgcaa ggcgcggatt atgccggaac
     9841 gatcctcgag accctgggaa gcaccttccc gcgcgacgaa ttgctggtga tctgcactga
     9901 cgagtcccac aaccgggaat tctttgcgcc gatcaccgcg gccttccccc gtcatgtctt
     9961 tctcgacgag ttcatcgtgg gcgaatttgc acgggaattc ctgggtctgc cgttcaccga
    10021 cgagcagtcg ctgggcttcg tgtgcaatat cgtgatgtgg gatgcgcgcg aattctccgg
    10081 cacgcccggc agcacctaca gcggcatgat ccatcggcac tggtactcca gacgtcgtgc
    10141 gcaggatgcg cgttcagacc ctccgtttcg cttcatcaac tgcggagaag caggtaaagg
    10201 cggcagcgaa cccggctatt tcgagaacgg cgctttcgtc gagtgccgca agggacattt
    10261 cagctggacc cgtaccgcca tccgccgggc gagtgagacc ctgtcgtggt accgggaatg
    10321 gccggagtgc gcgattgact cccgatgacc ctgggttccg ggcggcgaat tgttcttcgg
    10381 tacagtgtcg agttgaccgg tccccgccta cactttcgct tcagcatgcc caacgcttga
    10441 gccgtcgtcg tcacaggagc gcgtacaaat gccaactggt cagcccatcg cgaccgtcgt
    10501 cacacttacc ggagcagcct ttgcgcgcga cgccgaaggc agcttgcgca ggctgcgagt
    10561 cggcgagacg gttcgccagg gcgaggagat cgtcactgaa agcggcgccc gtgtcgaaat
    10621 agagttcctt agcggcggga cgctggccat cggtgaaagc aggacgattg cgatcagtcc
    10681 cgaactggcg ccggaggagg cgcctgacgc caccgagagt gcagtcgatt ccgccagcgt
    10741 ggaacagttg ctgcagtccc tgggtgatag tggggacctc ctggataagc tgcctgcgac
    10801 gtctgccgga tttgacggcg gcgttcctgg tgacgggcac gacttcgttc gggttctgcg
    10861 gatcgtggag agcgtagagc cgactccgtc acccacaccg gcgccgacgc cagagcccac
    10921 gccggtcccg acacccgcac cgaccccggc t
//
LOCUS       DAORUQ010000105        10897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6264_length_10897_cov_6.827430, whole genome shotgun sequence.
ACCESSION   DAORUQ010000105 DAORUQ010000000
VERSION     DAORUQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_10897_cov_6.827430"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..513
                     /gene="rpsJ"
                     /locus_tag="PLW13_10070"
     CDS             202..513
                     /gene="rpsJ"
                     /locus_tag="PLW13_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQY70766.1"
                     /translation="MQNQRIRIRLKAFDHRLIDISAQEIVDTAKRTGAQVRGPIPLPT
                     RKEKYTVLISPHANKDARDQYEIRTHKRVLDIVEPTEKTVDALMKLDLAAGVEVQISL
                     G"
     gene            610..1245
                     /gene="rplC"
                     /locus_tag="PLW13_10075"
     CDS             610..1245
                     /gene="rplC"
                     /locus_tag="PLW13_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQY70767.1"
                     /translation="MTIGIVGRKTGMTRVFTADGTSIPVTVVEATPNRVTQVKTADTD
                     GYSAVQITVGERKASRVSKPEAGHFAKAGVAAGRGLWELRLADGVDSPAVGEEITVSS
                     FEAGQKVDVTGQSRGKGFQGVIKRWNFRMQDATHGNSRSHRAPGSIGQNQSPGRVFKG
                     KKMAGHMGDERVTVQTLEVVRVDVERNLLLIKGAIPGAPGADVIVRPAEKA"
     gene            1268..1885
                     /gene="rplD"
                     /locus_tag="PLW13_10080"
     CDS             1268..1885
                     /gene="rplD"
                     /locus_tag="PLW13_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017135963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQY70768.1"
                     /translation="MELSITAPGNASAGTVQVSDVTFAREFNEDLVHQAVVAFMSGAR
                     QGSRQQKTRSEVSGGGRKPFKQKGSGRARAGSIRSPLWRSGGVTFAAVPQDHSQKLNR
                     KMYRAAMRSILSELARQDRLVVVESIALAEPKTKLLIEQLAGYRLERALIVADEVDRN
                     LYLASRNIPHVEVRDVAGVDPVSLVGCEKVLVTVAALRKFEEMLG"
     gene            1882..2178
                     /gene="rplW"
                     /locus_tag="PLW13_10085"
     CDS             1882..2178
                     /gene="rplW"
                     /locus_tag="PLW13_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQY70769.1"
                     /translation="MNQERVFNVLLGPHVSEKSAAATELANQYAFRVARDATKPEIKT
                     AVEQLFKVKVSSVRVLNVKGKVKLNKTGVAKRSDWKKAYVRLESGQDIDFTVTD"
     gene            2194..3021
                     /gene="rplB"
                     /locus_tag="PLW13_10090"
     CDS             2194..3021
                     /gene="rplB"
                     /locus_tag="PLW13_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQY70770.1"
                     /translation="MAVVKKKPTSPGRRFVVQVVNPDLHKGAPHRALVEAQGRNGGRN
                     NAGRVTVRHQGGGHRQHYRLVDFKRTKDGIPAKVERLEYDPNRTAHIALLLYRDGERR
                     YVIASRNMKAGDEVVSGENAPIKEGNALPLRNIPLGSVVHCIEMKPGKGAQIARSAGA
                     GVQLVAREGQYATLRLRSGEMRKVLVDCRATIGEVSNSEHNLRSLGKAGAKRWRGVRP
                     TVRGVVMNPVDHPHGGGEGRTSGGRHPVSPWGTPTKGYKTRSNKRTDKMIVRRRGKK"
     gene            3063..3338
                     /gene="rpsS"
                     /locus_tag="PLW13_10095"
     CDS             3063..3338
                     /gene="rpsS"
                     /locus_tag="PLW13_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQY70771.1"
                     /translation="MPRSLKKGPFIDLHLVKKVEVAAAKGDKRPIKTWSRRSMVSPDM
                     VGLTIAVHNGKQHVPVHVSEEMVGHKLGEFAATRTYKGHAGDKKTKK"
     gene            3362..3694
                     /gene="rplV"
                     /locus_tag="PLW13_10100"
     CDS             3362..3694
                     /gene="rplV"
                     /locus_tag="PLW13_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQY70772.1"
                     /translation="MEVMAKLSGARISAQKARLVADQIRGKPVEEALNTLAFSTKKAA
                     VLVKKVLNSAIANAEHNEGADVDELKVSSVFVDEGTTLKRIMPRAKGRADRIAKRTCH
                     ITVKVAGR"
     gene            3713..4372
                     /gene="rpsC"
                     /locus_tag="PLW13_10105"
     CDS             3713..4372
                     /gene="rpsC"
                     /locus_tag="PLW13_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQY70773.1"
                     /translation="MGQKVNPTGIRLGIVRKHNSVWYADSKNYAKNLITDLKARAYIE
                     SRLKNASVSRVVIERLAQTARVIVHTARPGIVIGKKGEDVDRLRKDLTQRMGVPVQIN
                     IEEVRKPDLDAKLLAQGVAQQLERRIMFRRAMKRVVQSAMRAGAEGVKVQVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYATFEALTTYGIIGVKVWVFKGEVIGRPGETQLAA
                     K"
     gene            4406..4819
                     /gene="rplP"
                     /locus_tag="PLW13_10110"
     CDS             4406..4819
                     /gene="rplP"
                     /locus_tag="PLW13_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQY70774.1"
                     /translation="MLQPKRTKFRKQFKGRNRGLAQRGSKVSFGEFGLKTTDRGRLTA
                     RQIESARRALTRHIKRGGKIWIRVFPDKPISKKPLEVRMGAGKGSVEYWVAQIQPGKV
                     LYEIEGVSEELAREALALAAAKLPIPTVFVKRTVM"
     gene            4819..5010
                     /gene="rpmC"
                     /locus_tag="PLW13_10115"
     CDS             4819..5010
                     /gene="rpmC"
                     /locus_tag="PLW13_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQY70775.1"
                     /translation="MNVSELRGKSVEDLNKELLRLLEARFKLRMQKATGQLGQTHLLK
                     AQRHDIARVKTILKEKAGK"
     gene            5013..5288
                     /gene="rpsQ"
                     /locus_tag="PLW13_10120"
     CDS             5013..5288
                     /gene="rpsQ"
                     /locus_tag="PLW13_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQY70776.1"
                     /translation="MTAEASKSVRTVTGRVVSDKMDKTITVLIERRVKHPVYGKYITR
                     SSKVHAHDEKNECRTGDLVTVRETRPISRSKTWALVSIDERPVRVDA"
     gene            5340..5708
                     /gene="rplN"
                     /locus_tag="PLW13_10125"
     CDS             5340..5708
                     /gene="rplN"
                     /locus_tag="PLW13_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQY70777.1"
                     /translation="MIQTESYLEVADNSGARRVMCIKVLGGSHRRYARVGDVIKVTVK
                     DAIPRGRVKKGQVMNAVVVRTRKGVRRPDGSIIRFDDNAAVLLNNQLAPIGTRIFGPV
                     TRELRTEKFMRIISLAPEVL"
     gene            5738..6055
                     /gene="rplX"
                     /locus_tag="PLW13_10130"
     CDS             5738..6055
                     /gene="rplX"
                     /locus_tag="PLW13_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQY70778.1"
                     /translation="MAKIRRNDEVIVITGKDKGKRGKITRVLENGRVVVAGINMVKRH
                     TRANPQLGTAGGIIGKEASVHASNVALVNPVSGKADRVGFKVLEDKTKVRVFRSSGEL
                     IDG"
     gene            6061..6600
                     /gene="rplE"
                     /locus_tag="PLW13_10135"
     CDS             6061..6600
                     /gene="rplE"
                     /locus_tag="PLW13_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQY70779.1"
                     /translation="MARFKEIYNSEIVPKLMGEFGYQSVMQVPRITKITLNMGVGEAV
                     GDKKILDNAMGDMAKISGQKPVMTKAKTSIAGFKIREGWPVGCKVTLRRERMYEFLER
                     LIDIAIPRIRDFRGISAKSFDGRGNFSMGVSEQIIFPEIDYDKIDALRGMDIAIATTA
                     RTDDEARALLRAFNFPFRG"
     gene            6608..6913
                     /gene="rpsN"
                     /locus_tag="PLW13_10140"
     CDS             6608..6913
                     /gene="rpsN"
                     /locus_tag="PLW13_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQY70780.1"
                     /translation="MAKTSMIMREKKRQRTVAKYARKRAALKESIRDPKLSDEARWEA
                     TQALQKLPRDASPSRLRTRCEITGRPNGVYRRFGLGRNKLREAAMRGDIPGLVKSSW"
     gene            6936..7331
                     /gene="rpsH"
                     /locus_tag="PLW13_10145"
     CDS             6936..7331
                     /gene="rpsH"
                     /locus_tag="PLW13_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQY70781.1"
                     /translation="MSMQDPIADMLSRIRNAQARAKPSVKMPSSTKKVAIARVLRDEG
                     YVAGFKVTAEDGKPVLEVTLKYFEGKAVIAEIDRVSRSSLRRYAGKDELPKVRGGLGI
                     AIVSTSKGVMTDRAARAAGVGGEVLCTVF"
     gene            7361..7894
                     /gene="rplF"
                     /locus_tag="PLW13_10150"
     CDS             7361..7894
                     /gene="rplF"
                     /locus_tag="PLW13_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQY70782.1"
                     /translation="MSRVAKNPVILPKGVEVAFNGNAITVKGSKGSLEMTVHESVQVT
                     QEDNVLSFAPRTAETSADALAGTMRVLVGNMVAGVSSGFERKLQLIGVGYRAAAKGDT
                     VSLTLGLSHPVEYALPKGVTAETPTQTEIVLKSADKQLLGQAAAEIRSYRPPEPYKGK
                     GIRYSDENVHRKEAKKK"
     gene            7908..8255
                     /gene="rplR"
                     /locus_tag="PLW13_10155"
     CDS             7908..8255
                     /gene="rplR"
                     /locus_tag="PLW13_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQY70783.1"
                     /translation="MNKQTARVRRAKRSRAKMRELGVYRLSVHRTPRHIYAQVISPAG
                     DRVLASASTLDKELRSGSTGNVEAATAVGKLVAERARAAGVSQVAFDRAGFRYHGRVK
                     ALADGAREGGLEF"
     gene            8265..8774
                     /gene="rpsE"
                     /locus_tag="PLW13_10160"
     CDS             8265..8774
                     /gene="rpsE"
                     /locus_tag="PLW13_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQY70784.1"
                     /translation="MAFEQQDSGANEGFQEKLVQVNRVAKVVKGGRIFAFTALTVVGD
                     GKGKVGFGRGKAREVPAAIQKAMDAARRNMIQVDLDRGTIQYAVRAAHGASKVYMQPA
                     SEGTGVIAGGAMRAVLEIAGVHNVLAKCYGSTNPVNVVRATIAALKSVSSPREVAAKR
                     GKSVADITG"
     gene            8792..8983
                     /gene="rpmD"
                     /locus_tag="PLW13_10165"
     CDS             8792..8983
                     /gene="rpmD"
                     /locus_tag="PLW13_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQY70785.1"
                     /translation="MTNHEKTIKVTLVRSTAGRLQSHRACVKGLGLRRIGHVVQVEDT
                     PAVRGMINRVNYLLKVEGA"
     gene            9001..9435
                     /gene="rplO"
                     /locus_tag="PLW13_10170"
     CDS             9001..9435
                     /gene="rplO"
                     /locus_tag="PLW13_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQY70786.1"
                     /translation="MRLNTLSPAPGRRHAPKRVGRGIGSGLGKTCGRGHKGQKARKSG
                     NVRPGFEGGQMPLQQRLPKYGFTSRIARVTAQLRTAELNQVEGEIVDLETLKAANVIS
                     RNIERVRVFASGEVRRALQLKGIAVTAGARSAIEAAGGKVED"
     gene            9436..10755
                     /gene="secY"
                     /locus_tag="PLW13_10175"
     CDS             9436..10755
                     /gene="secY"
                     /locus_tag="PLW13_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQY70787.1"
                     /translation="MAKLPLGGVNQAGLGELWGRIRFLFLGLIVYRIGTHIPVPGINP
                     DQLKVLFNQNQGTIVGLFNMFSGGALERMSVMALGVMPYISASIIVQLMSSVLPQLEQ
                     LKKEGEAGRRKITQYTRYGTLLLAAVQASGMAVGMGAQGLFYASGFSVIFVAIVSLVA
                     GAMFMMWLGEQITERGLGNGISMLIFAGIVAGLPAALGQTLEQARQGELNILVLLVIL
                     AIAVGVIFLIVRIERGQRRITVNYAKRQVGRRVVQGQASHLPLKVNMAGVIPAIFASS
                     ILLFPASIAQWFGQSASGMEWLQNLAFMIGPGQPLNIVLFTALIVFFCFFYTALVFNP
                     KEVADNLKKSGAYVPGIRPGEQTAKFIDSVLSRLTLFGAAYIAAVCLLPQFLVVVWNV
                     PFYLGGTSLLIVVVVVMDFMAQVQSHLMSHQYDSLLKKSNLQSFGRR"
     gene            10780..10896
                     /gene="rpmJ"
                     /locus_tag="PLW13_10180"
     CDS             10780..10896
                     /gene="rpmJ"
                     /locus_tag="PLW13_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQY70788.1"
                     /translation="MKVQASVKKVCRNCKIVRRKGAVRVICKVEPRHKQRQG"
BASE COUNT         2194 a         3206 c         3784 g         1713 t
ORIGIN      
        1 ggcgggcgca cggtgggcgc gggcgtggtg gcgaagatca tcgagtgatc cgccgaaaaa
       61 cgcagcgtga atagttggaa tttctgcagc ccccggcgaa ttgtgttgac acaccggggt
      121 gctgaaaata gaattccgct tcttttttga ctgcccccga aacgggacag atgaaggctt
      181 gaaccggagc tcagtggtca gatgcaaaat cagcgtatcc gaattcgact caaggctttt
      241 gaccatcgcc tcatcgacat ttccgcccag gagatcgtcg acacggccaa gcgcacgggt
      301 gcgcaggtcc gcggtccgat tccgctgccc acgcgcaagg agaagtacac ggtcctgatc
      361 tcgccgcacg ccaacaagga tgcgcgcgat cagtacgaga tccgcaccca caagcgcgtg
      421 ctggacatcg tggagccgac ggaaaagacc gtcgatgcgt tgatgaagct ggatctcgca
      481 gccggcgtag aggtgcagat cagtctcggt tgagaatgcc gggacctgcc cggccgtgac
      541 cgggcatgtg taacgcacgc tcgcgtgcgg ccatagaggg ttcagccccg tacacttgag
      601 gatgacaaga tgactattgg tattgtcggt cgcaagaccg gtatgacccg cgtgttcacc
      661 gctgacggga cttcgatccc cgtcacggtc gtcgaagcaa cgcccaaccg cgttacccag
      721 gtcaagaccg ccgacaccga cggctacagc gccgtgcaga tcaccgtcgg cgagcgcaag
      781 gcctcgcgcg tcagcaagcc cgaagccggg cacttcgcca aggccggcgt cgccgccggg
      841 cgcggcctgt gggagcttcg tctcgccgac ggcgttgact ctcccgcggt cggcgaggaa
      901 atcaccgtct cctccttcga agcggggcag aaggttgacg tgacgggcca gtcgcgcggc
      961 aagggattcc agggcgtgat caagcgctgg aacttccgca tgcaggacgc gacgcacggt
     1021 aactcgcgct cgcaccgcgc acccggctcg atcggtcaga accagtcgcc gggccgcgtg
     1081 ttcaagggca agaaaatggc aggccacatg ggcgacgagc gcgtgaccgt gcagaccctc
     1141 gaggtcgtgc gcgtggacgt cgaacgcaat ctgctgctca tcaaaggtgc aatcccgggc
     1201 gcgccggggg ccgacgtcat cgtgcgtccg gccgagaaag cctgaggatc cctgggggta
     1261 tatgcgcatg gaattgagta tcaccgctcc tggcaacgca tccgcgggaa ccgtgcaggt
     1321 ttccgacgtg actttcgccc gggaattcaa cgaggacctg gtccaccagg ccgtggtcgc
     1381 gttcatgtcg ggtgcccgcc agggttcccg ccagcagaag acgcgctcgg aagtgagcgg
     1441 cggcggccgc aagccgttca agcagaaggg ctcgggccgc gcccgcgccg gcagcatccg
     1501 cagcccgctg tggcgctcgg gcggcgtgac cttcgccgcg gtgccgcagg accactcgca
     1561 gaagctgaac cgcaagatgt atcgcgcggc gatgcgctcg atcctgtccg agctggcgcg
     1621 ccaggaccgc ctggtcgtgg tggagtccat cgccttggcc gagccgaaga ccaagctgct
     1681 gatcgagcag ctcgccggct accggctcga gcgcgcgctg atcgttgcgg acgaagtgga
     1741 tcgcaacctt tacctggcgt cgcgcaacat tccgcacgtc gaggttcgtg acgtggcggg
     1801 cgtggatccg gtcagcctgg tcgggtgcga gaaggtgctg gtcaccgtgg cggcgctgcg
     1861 caagttcgag gagatgctgg gatgaatcag gagagagtgt tcaacgtcct gctcgggccg
     1921 catgtctcgg agaaatccgc ggcggccacc gagctggcga accagtacgc cttccgtgtt
     1981 gcacgcgatg ccaccaagcc ggaaatcaag acggcggtgg agcagctctt caaggtcaag
     2041 gtttccagcg tccgtgtcct gaacgtcaag ggcaaggtca agctgaacaa gaccggtgtg
     2101 gcgaagcgct ccgactggaa gaaggcgtat gtgcgcctgg agtccggcca ggacatcgac
     2161 ttcacggtca cggattaaac gggagcaggg aagatggcag tcgtaaagaa gaagccgact
     2221 tcgccgggca ggcgtttcgt ggtgcaggtg gtcaatccgg acctgcacaa gggcgcaccg
     2281 catcgtgcgc tggtcgaggc gcagggcagg aacggcgggc gcaacaacgc cggtcgcgtc
     2341 accgtgcgcc accagggcgg cgggcatcgc cagcattatc gcctggtgga tttcaagcgc
     2401 accaaggatg gcataccggc caaggtcgag cgcctggaat acgacccgaa ccgcaccgcg
     2461 catatcgcgc tgctgctgta ccgtgatggc gagcgccgct acgtgatcgc ctcgcgcaac
     2521 atgaaggcgg gcgatgaggt ggtatcgggc gagaacgcgc cgatcaagga aggcaacgcg
     2581 ctgccgttgc gcaatatccc gctgggctcc gtggtgcatt gcatcgagat gaagccgggc
     2641 aagggcgccc agatcgcccg cagcgccggt gccggcgtgc agctggtggc ccgcgaaggc
     2701 cagtacgcga cgctgcgcct gcgttcgggc gagatgcgca aggtgctggt cgactgccgc
     2761 gcgaccatcg gcgaagtgtc gaattcggag cacaacctgc gctcgctcgg caaggccggc
     2821 gcgaagcgct ggcgcggcgt gcgcccgacc gtgcgcggtg tcgtgatgaa cccggtcgac
     2881 cacccgcacg gcggtggcga agggcgcacc agcggcggcc gtcatccggt cagcccgtgg
     2941 ggcactccga ccaagggtta caagacgcgg tccaacaagc gcaccgacaa gatgatcgtg
     3001 cgccgccgcg gcaagaaata aggcgccagt caagcgcgaa acagctcgag aggattcagt
     3061 ctgtgccacg ttcgttgaag aaaggaccgt tcatcgacct tcatctcgtg aagaaggtcg
     3121 aggtcgccgc cgccaagggc gacaagaggc cgatcaagac ctggtcgcgc cgttcgatgg
     3181 tgtcccccga catggtgggc ctgaccattg ccgtccacaa cgggaagcag catgtgccgg
     3241 tgcacgtctc cgaggagatg gtcgggcaca agctgggtga atttgccgcc acgcgtacct
     3301 acaagggtca cgccggcgac aagaagacga agaagtaagc gtaccgggcg agggtccaac
     3361 gatggaagtc atggcaaaac tcagtggcgc gcggatatcc gcccagaagg cccgcctggt
     3421 ggcggaccag attcggggca agcccgtcga agaggcgctg aacacactcg cgttcagcac
     3481 caagaaggca gctgtgctcg tgaagaaagt gctgaattcc gcgatcgcta acgcggagca
     3541 caacgagggc gccgatgtcg acgagctgaa ggtcagttcg gtgttcgtgg acgagggcac
     3601 gacgctcaag cgcatcatgc cgcgtgccaa gggtcgcgca gatcgcattg cgaagcgcac
     3661 ctgtcacatt acggtcaaag ttgccggtcg ctgaggcaaa acgggagaca cgatgggtca
     3721 gaaagtcaac cctaccggga ttcggctcgg tatcgtccgc aagcacaatt cggtgtggta
     3781 tgcggacagc aagaactacg cgaagaacct gatcacggat ctcaaggcgc gtgcgtacat
     3841 cgagtcgcgc ctgaagaacg catcggtcag ccgcgtggtg atcgagcgtc tggcgcagac
     3901 tgcgcgggtc atcgtgcaca cggcgcgccc gggcatcgtg atcggcaaga agggcgagga
     3961 tgtcgatcgc ctgcgcaagg atctgacgca gcgcatgggc gtcccggtgc agatcaacat
     4021 cgaggaagtg cgcaagcccg acctggatgc gaagctcctg gcgcagggcg tggcccagca
     4081 gctcgagcgg cggatcatgt tccgtcgcgc gatgaagcgg gtcgtgcaga gcgcgatgcg
     4141 cgccggcgcc gaaggcgtga aggtgcaggt cagcggccgc ctgggcggcg ccgagatcgc
     4201 ccgcacggaa tggtaccgcg agggacgcgt tccgctgcac acgctgcgtg ccgacatcga
     4261 ctacgcgacc ttcgaggcgc tcacgaccta cggcatcatc ggtgtcaagg tgtgggtgtt
     4321 caagggcgaa gtgatcggcc ggccgggcga aacacagctg gcggccaagt aaggccggac
     4381 gaaacaattc cgagagcaga aagcgatgtt acagccgaag cgtaccaagt ttcgcaagca
     4441 gttcaagggg cgcaaccgtg gcctggccca gcgtggcagc aaggtgagct tcggggaatt
     4501 cgggttgaag acgaccgatc gcggccgcct cacggcacgc cagatcgagt cggcccgtcg
     4561 cgcgctgacc cgccacatca agcggggcgg caagatctgg atccgcgtgt tccccgacaa
     4621 gccgatctcc aagaagccgc tggaagtgcg gatgggcgcg ggcaagggca gcgtggagta
     4681 ctgggtggcc cagatccagc ccggcaaggt gctctatgaa atcgagggag tgagcgagga
     4741 actcgcgcgc gaagcgctcg cgctggccgc cgcgaagctg cccattccga ctgtattcgt
     4801 gaaaagaacg gtgatgtgat gaacgttagc gaactgcgcg ggaagtccgt ggaagacctg
     4861 aacaaggagc tgctgcgcct gctcgaagcg cgcttcaagc tgcgcatgca gaaggcgacg
     4921 gggcagcttg gccagacgca tctgttgaag gcccagcggc acgatattgc gcgggtcaag
     4981 acgatcctca aggaaaaggc aggtaagtga ccatgacggc tgaagcgagc aagagcgtgc
     5041 gcacggtgac gggccgcgtt gtcagcgaca agatggacaa gacgatcact gtcctgatcg
     5101 agcgtcgcgt gaagcacccg gtatacggca agtacatcac gcgctcgtcc aaggtgcacg
     5161 cccacgacga gaagaacgag tgccgcaccg gcgacctggt gacggtgcgg gaaacgcgtc
     5221 cgatctcgcg ctccaagacc tgggcgctgg tgagcatcga cgagcgtccc gtgcgcgtgg
     5281 atgcgtgagc ggcagacggc acaagcgata gcggcggcag tggcgagttg gagtagaaca
     5341 tgattcagac tgaatcgtac ctggaagtgg cggacaacag cggcgcgcgt cgcgtgatgt
     5401 gcatcaaggt gctcggcggt tcgcaccgcc gctatgcgcg ggtcggtgac gtgatcaagg
     5461 tcacggtaaa ggatgcgatc ccgcgcggtc gcgtcaagaa gggccaggtg atgaacgcgg
     5521 tggtggtgcg cacccgcaag ggcgtacgcc gccccgacgg ttcgatcatc cgcttcgacg
     5581 acaacgcggc cgtgctgctg aacaaccagc tggcaccgat cggcacgcgc atcttcgggc
     5641 cggtgacgcg cgagctgcgc accgaaaagt tcatgcgcat catttcgctg gctcccgaag
     5701 tgctctgagc acgacggcaa cggcaagagg caaggcaatg gcaaagatca ggcgcaatga
     5761 cgaggtgatc gtcatcaccg gaaaggacaa gggcaagcgc ggcaagatca cgcgcgtgct
     5821 cgagaacggc cgcgtggtcg tggcggggat caacatggtc aagcggcaca cgcgtgccaa
     5881 cccgcagctc ggcaccgccg gcggcatcat cgggaaggaa gcgtccgtgc acgcctccaa
     5941 cgtggcgctg gtgaaccccg tgagcggcaa ggccgatcgc gtgggcttca aggtgctcga
     6001 ggacaagacc aaggtgcggg tgttccgctc ctccggtgaa ctcatcgacg ggtaatggca
     6061 atggcaaggt tcaaagagat ctacaacagc gaaatcgtcc ccaagctgat gggcgagttc
     6121 ggctaccaga gcgttatgca ggtgccccgc atcaccaaga tcaccctgaa catgggtgtg
     6181 ggcgaggccg tgggcgacaa gaagatcctg gacaacgcca tgggcgacat ggccaagatc
     6241 tccggtcaga agccggtcat gaccaaggcc aagacctcga tcgccggctt caagatccgc
     6301 gaaggctggc cggtgggctg caaggtcacg ctgcgccgcg agcgcatgta cgaattcctc
     6361 gagcgcctga tcgacatcgc gatcccgcgc atccgggact tccgcggcat cagcgccaag
     6421 agcttcgacg gtcgcggcaa tttctcgatg ggcgtatccg agcagatcat cttcccggag
     6481 atcgattacg acaagatcga cgccctgcgc ggcatggaca tcgcgatcgc gacgacggcg
     6541 cgcaccgatg acgaagcgcg cgcgttgctg cgggcattca actttccttt caggggctga
     6601 gtctcagatg gccaagacgt cgatgatcat gcgggagaag aagcgccagc gcaccgtggc
     6661 gaaatacgcc aggaagcgtg ccgcgctgaa ggaaagcatc cgcgatccga aactgagcga
     6721 cgaagcgcgc tgggaagcga cgcaggcgct gcagaagctg ccgcgcgatg cgagcccctc
     6781 gcggctgcgc acccgttgcg aaatcacggg ccgtccgaac ggtgtgtatc gccgcttcgg
     6841 tctgggccgc aacaaattgc gcgaggcggc catgcgcggg gacatcccgg gcctggtcaa
     6901 gtccagctgg taagtccacg cgaggtggaa tcgatatgag catgcaggat cctattgcgg
     6961 acatgctgag ccgcattcgc aacgcccagg cgcgtgcgaa gccctcggtg aagatgccct
     7021 cgtcgaccaa gaaggtcgcc attgcgcgcg tcctgaggga cgaaggctat gttgccggtt
     7081 tcaaggtcac tgccgaggac ggcaagccgg ttctcgaggt gacgctgaag tatttcgaag
     7141 gcaaggcggt gatcgccgag atcgaccgcg tgagccgctc gagcctgcgc cgctatgccg
     7201 gcaaggacga gctgccgaaa gtgcgcggcg gccttggcat tgccatcgtg tcgaccagca
     7261 agggcgtgat gactgaccgg gcggcccgcg cggccggtgt cggcggtgaa gtcctttgca
     7321 ccgtgttctg atgtgacgaa ttgaccgaca ggtaagaacc atgtccagag ttgcgaagaa
     7381 tcccgtaatc ctgcccaagg gcgtcgaagt ggcgttcaat ggcaacgcga ttacggtcaa
     7441 gggaagcaag ggctccctgg aaatgaccgt gcacgagtcg gtgcaggtaa cccaggaaga
     7501 caacgtgctg agcttcgcgc cgcgcaccgc cgagacctcg gccgatgcgc tcgccggaac
     7561 catgcgcgtg ctcgtcggca acatggttgc cggcgtctcg agcggtttcg aacgcaagct
     7621 gcagctgatc ggcgttggtt accgcgccgc ggcgaagggc gacaccgtga gcctcacgct
     7681 cgggctgtcg cacccggtcg aatacgcact gccgaaaggc gtgacggccg agacgccgac
     7741 ccagaccgag atcgtgctga agagcgcgga caagcagctg ctcgggcagg cggctgcgga
     7801 gattcgttcg taccggccgc cggagcccta caagggcaaa ggcatccgtt attcggatga
     7861 gaatgttcat cgcaaggaag cgaagaagaa gtagggccaa agacatgatc aacaagcaga
     7921 cagcaagagt gcgtcgcgcc aagcgcagcc gcgccaagat gcgcgaactg ggcgtttatc
     7981 gtctcagcgt gcaccggacc ccgcgtcata tctacgcgca ggtcatctcg cccgcgggcg
     8041 acagggtgct cgcgagcgcg tccacgctgg acaaggagct gcgcagcggc agcaccggca
     8101 acgtggaagc ggcgacggcg gtcggcaagc tggttgccga gcgcgccagg gcggcgggcg
     8161 tgagccaggt tgccttcgat cgcgcgggct tccggtatca cgggcgcgtg aaggctctgg
     8221 ccgatggtgc gcgcgaaggc ggattggaat tctagggtaa gactatggcg ttcgagcagc
     8281 aggattcagg tgctaacgag ggcttccagg agaagctggt ccaggtcaac cgcgtggcca
     8341 aggtcgtgaa gggcggacgc atattcgcgt tcaccgcgct cacggtcgtg ggcgacggca
     8401 agggcaaggt cggcttcggc cgcggcaagg cgcgcgaagt gccggccgcg atccagaagg
     8461 cgatggatgc ggcgcgccgc aacatgatcc aggtcgacct cgatcgcggc acgatccagt
     8521 atgcggtgcg agccgcgcat ggcgcctcca aggtgtacat gcagccggcg tccgagggta
     8581 ccggtgtcat cgccggcggt gcgatgcggg ccgtgctgga aattgccggc gtgcacaacg
     8641 tgctggccaa gtgctacggc tcgaccaacc cggtcaacgt ggtgcgtgcg accattgccg
     8701 cgctgaagtc cgtgagttcg ccgcgcgaag tggcggccaa gcgtggcaag agcgtggccg
     8761 atatcaccgg ctgaggcaaa ggacacaggt catgacgaac cacgaaaaga caatcaaggt
     8821 tacgctggtg cgcagcaccg cgggcaggct ccagagccac cgcgcctgcg tcaaggggct
     8881 ggggctgcgc cgtatcgggc acgtggtcca ggtcgaggac acgccggcgg tacgcggaat
     8941 gatcaacagg gtcaattacc tgttgaaagt tgaaggtgca tgatcatgag cgatgaaacg
     9001 atgcgtctga acacgctgag ccccgcgccg ggtcgccgcc acgcgcccaa gcgcgtcggg
     9061 cgcggcatcg gcagcggtct cggcaagacc tgtggccgtg gccacaaggg tcagaaggca
     9121 cgcaaaagcg gtaacgtgcg ccccggcttc gaaggcggcc agatgccgct gcagcagcgc
     9181 ctgccgaaat acggcttcac ctcgcgcatc gcgcgcgtga cggcacagct gcgcaccgcg
     9241 gagctgaacc aggtcgaggg tgagatcgtc gacctcgaaa cgctgaaagc ggcaaacgtg
     9301 atctcgcgca acatcgagcg cgttcgcgtg ttcgcctcgg gcgaggttcg cagggcgctc
     9361 cagctgaagg gaatcgccgt gacggccggt gcgcgcagcg cgatcgaagc ggcaggcggc
     9421 aaggtcgagg actaaatggc aaagctgccg ctgggcgggg tgaaccaggc gggcctgggc
     9481 gagctctggg gccggatccg gttcctgttt ctcgggctga tcgtctatcg catcgggacg
     9541 cacatcccgg tgcccgggat caacccggac cagctgaagg tgctgttcaa ccagaaccag
     9601 gggaccatcg tcggcctctt caacatgttc tcgggcggtg cgctggaacg gatgagcgtc
     9661 atggcgctcg gtgtgatgcc ctacatctcg gcatcgatca tcgtgcagct gatgtcctcg
     9721 gtgctgccgc agctggaaca gttgaagaag gaaggcgagg ccggacggcg caagataacg
     9781 cagtacacgc gttacgggac gctgttgctg gccgcggtac aggccagtgg catggcggtc
     9841 ggcatgggcg cgcaggggct gttctacgcg agcggcttca gcgtgatatt cgtcgccatc
     9901 gtatcgctgg tggcgggcgc gatgttcatg atgtggctcg gcgagcagat caccgagcgc
     9961 ggcctgggca acggcatctc gatgctgatc ttcgccggca tcgtggcggg cctgccggcg
    10021 gcgctcggcc agacgctgga gcaggcgcgc cagggggagc tgaacatatt ggtgctgctg
    10081 gtgatactgg cgatcgcggt gggtgtgatc ttcctgatcg tgcgcatcga gcggggccag
    10141 cggcgcatca cggtgaatta cgccaagcgc caggtcggac ggcgggtggt gcaggggcag
    10201 gcgagccacc tgccgctgaa ggtgaacatg gcgggcgtga tacccgcgat cttcgccagc
    10261 agcatcctgc tgttcccggc ctcgatcgcg caatggttcg ggcagtcggc gtccggaatg
    10321 gaatggttgc agaacctggc cttcatgatc gggccgggcc agccgctcaa catcgtgttg
    10381 ttcacggcgt tgatcgtgtt cttctgcttc ttctatacgg cgttggtgtt caacccgaag
    10441 gaagtggcgg acaacctgaa gaaatccggc gcctatgtgc cggggatccg tccgggcgag
    10501 cagaccgcga agttcatcga cagcgtgctc agccggctga cgttgttcgg tgcggcgtat
    10561 atcgcggcgg tgtgtctgct gccgcagttc ctggtcgtgg tatggaacgt gccgttctac
    10621 ctcggcggga cctcgctgct gatcgtggtg gtggtcgtga tggatttcat ggcgcaggtc
    10681 cagtcgcacc tgatgtcgca ccagtacgac tcgctgctga agaaatcgaa cctgcagagt
    10741 tttgggcgcc gttgaggcgc gtgttgaagg agatccggga tgaaagtaca ggcttccgtc
    10801 aagaaagtgt gtcgcaactg caagatcgta cgtcgcaagg gcgcagtgcg cgtgatctgc
    10861 aaggtcgaac cgcgccacaa gcagcgccag ggctgag
//
LOCUS       DAORUQ010000106        10879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6286_length_10879_cov_5.973854, whole genome shotgun sequence.
ACCESSION   DAORUQ010000106 DAORUQ010000000
VERSION     DAORUQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6286_length_10879_cov_5.973854"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLW13_10185"
     CDS             complement(<1..491)
                     /locus_tag="PLW13_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70789.1"
                     /translation="MVALAMAGALAAAAAMIWREYRAARDGVFAHALLLAEADARTDR
                     AVQERDIALISERLARFMALPGVKYAAVRDADGREIVARRGEDADGYPEADFGRARRG
                     VAAFDQGVATDERRAGLERFSDLTVPVFVTAGMQRGAGPRASGGETGSRYLVGYYHLR
                     VSL"
     gene            complement(591..1823)
                     /locus_tag="PLW13_10190"
     CDS             complement(591..1823)
                     /locus_tag="PLW13_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY70790.1"
                     /translation="MDLVPYAVPFFLLAIGLEYAWGRARGNDTYRLNDSINSLSCGVL
                     STSIKLLGLDIGGRVFTQAEQHWALARLDAQSPWTWLLALLLYDFCYYWFHRISHERT
                     LLWAAHVVHHQSEEFNLTTALRQTSTGFLVGWVFYIPCFVAGVPAAVFVTVASAHLIY
                     QFWIHSRHIPKLGPLEWVLVTASNHRVHHAQNPAYVDKNYGGLLIVWDRLFGTFEEER
                     EAEPAVYGILGPLKSWNPLWANLHIYAQMLRDAWYTRRWSDKLRVLLARTGWRPDDVA
                     ARFPVVKRPLAEFRRYDPPLAPRAAWYAGLQFLALMAFALWLEQAAPAMHATGRAALF
                     ACLLFGLVAIGVMLEQLPRGRALERLRLGVTLAAVLGLAVTGVAAWPVAAVLIAYLAA
                     SVILLARTSSRGAALSDS"
     gene            1923..4136
                     /gene="selD"
                     /locus_tag="PLW13_10195"
     CDS             1923..4136
                     /gene="selD"
                     /locus_tag="PLW13_10195"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQY70791.1"
                     /translation="MQAHAPHPREPLSRELLLIGGGHSHVAVVRALGMRPVPGLRVTV
                     VSRDVHTPYSGMLPGLVAGHYGFDDCHIDLRPLCEASGVRLIHGEVDALDPARQSVRI
                     AGRESLRYDWASINIGSRPALGGIEGATAHGISVKPIDRFLAHWLALLEDLRANPRPC
                     RLLIVGGGAAGVEIALACAWRARQALGAQAALFSVGIVNAAPVLLPSHNALVRRYFRR
                     ELERRGIVLHSGARVAAVDAQGVMLADGRRLQAEQLVWATQAGAPAWPAAAGLACDAN
                     GFVRVDACLRSLSHPNVFAAGDIAALPEPCPKSGVYAVREGQALALGLRRVALGGEPV
                     VYRPQRRFLSLVSTGDRRAVVSRGPLFAAGAWAWRWKDRIDRAFMAMYRVEPAAPPEP
                     GEAPAMRCGGCAAKIGGDALGRVLADLARPPREGVEAGMDIGDDAAVLAPPPGQRLIQ
                     SVDYFRGFIDDPFLVGQVAAVHALGDLLAMGARPHSALAIATVPYAAARVMEQTLGQL
                     MQGALKTLDAEGVALVGGHSSEGAELAFGLSVNGYAAPGEILYKSGLRDGHALVLTKA
                     IGTGTLLAANGAAAAVGRWIDGALAGMTQSSRAAADILRRHGAAACTDITGFGLLGHL
                     LEMLRASGTGARLSIDAVPVLEGARECLARGYRSSLHESNRANLAALVHPLSAAPAQA
                     LEVLLDPQTAGGLLAGIPPARAQSCVAELRAAGYPACAVIGSCSAAAGPGGVLID"
     gene            complement(4126..5223)
                     /gene="mnmH"
                     /locus_tag="PLW13_10200"
     CDS             complement(4126..5223)
                     /gene="mnmH"
                     /locus_tag="PLW13_10200"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HQY70792.1"
                     /translation="MRPEIVGPEQFDTLLREGAHFIDVRAEIEFARGAIPGAVNLPIL
                     TTTEREQVGTTYKRKGQEAAVALGHRLVSGSTREERIARWCAVLQEHPDALVHCWRGG
                     MRSGLATQWIAEAGVAVRLVQGGYKALRHRLLRELEAPEPREPLYVIGGRTGSAKTDL
                     VKALPWGVDLEELAHHRGSSFGRRVGATPAQTDFENRLALLLLRRRHAAPGAALFLED
                     ESRTIGSISVPLELYQAMKAAPLVIIDAPFASRVEQILRDYICEDLRERVALDPEHGF
                     AQFGAALHESLQRIQRRLGGERYAMAEGLMRAALHQQGTRGDPSGHRAWIELLLRDYY
                     DPMYEYQLGKSAGRVVFRGDFAAVREWCLSR"
     gene            5361..5603
                     /locus_tag="PLW13_10205"
     CDS             5361..5603
                     /locus_tag="PLW13_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HQY70793.1"
                     /translation="MENSNTSFSRLQRGDRARVLGFGPMPEYYRNRLLSLGLTPGTEF
                     TVAREAPLGDPVQINLRGFRLSVRRAEAAGLEVELL"
     gene            5600..7918
                     /gene="feoB"
                     /locus_tag="PLW13_10210"
     CDS             5600..7918
                     /gene="feoB"
                     /locus_tag="PLW13_10210"
                     /inference="COORDINATES: protein motif:HMM:NF007105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HQY70794.1"
                     /translation="MNKPFTIALVGNPNCGKTTLFNRLTGTHQSVGNWPGVTVERKTG
                     SFTHEGRQFLVVDLPGTFSLHVSRDDDAIDQQIAQAYVLAREADLIVNIIDASSIARG
                     LYLSSQLFDASVPVVVALNMVDVAQSQGVHVDAHRLAACLGCPVVPLVASRGEGVRAL
                     IEEVCAGLQTRQPPPQRMALDATLESALLRVQAALDPADAGPAGRMLAGALLARDAAV
                     LERIGEAARARVLAIVDELEATLGGNAGDALIGARYQAIGTIVDQAVRTEDAKRFNLT
                     DALDRVLLNRVLAFPLLLGVMYLMFMFTINVGGAFIDFFDIAAGALFVEAPRLALDAL
                     GLPAWLVTAVADGVGGGVQLVASFIPIIATLFLFQSFLEDSGYMARAAFILDRLMRAI
                     GLPGKSFVPLIVGFGCNVPAVMATRSLDTQQDRLLTAMMAPFMSCGARLTVYALFAAA
                     LFQRNGQNVVFALYLIGIALAIVTGLLVRRRLLARDLSPFLLELPPYHVPTLRGLLTH
                     TWFRLQGFVLRAGKAIVAVVLLLNFVNSIGTDGSFGNQNTERSLLSAIGKSITPVFAP
                     MGLTEANWPATVGIFSGVFAKEVVVGTLDALYTNMARADDVAPEPVSIGAMLEEAVAS
                     VGTNLAALGDTFGDPLGMGNLDDSAAIAEEQAVHSGTLGLMATLFDGQLGAFSYVLFV
                     LLYMPCVATLGAIYKELGGFWAFFSATWNTVIAYTLAVTVYQAGTFAAHPVSSAAWIA
                     GCAAALLGCYAWLMHLGRQRAGSGKLIPVVNL"
     gene            complement(7932..8825)
                     /locus_tag="PLW13_10215"
     CDS             complement(7932..8825)
                     /locus_tag="PLW13_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HQY70795.1"
                     /translation="MCRLYALMANEPTRAECSLVRSQNSLMRQSEGDAEGVVHGHGWG
                     VADYTDGAPSVEKQAWAAYHGEHFPRKAARVHAHAVVAHVRRATVGQTSVENTHPFRH
                     GRFTFAHNGTIPDFGQVRQRMLAETDPLHRAAIRGQTDSEHLFHYLLTRWSRGPQDDL
                     PGTVRAGLERVLAWCHEIAPGSKVGLNIVLSDGRQMVASRLNRSLWYLCRDAIVRCSV
                     CGRAHVHHEPGAAYRAVEVASEPVTPEEPWRLLPDASVLGVDPDYRLRILSLRVPAQS
                     APRRGSESLFGCAFGSRTMSA"
     gene            9017..9559
                     /locus_tag="PLW13_10220"
     CDS             9017..9559
                     /locus_tag="PLW13_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HQY70796.1"
                     /translation="MPRPFLPVIASVASLLLLGGCARAPGLPQETAPSAFVIERDLEG
                     ATVARGEFSAINGVRRGFTVYLEGVRSGDTFTLKERFEYDDGERDHKTWVLRLAGDGQ
                     YTGTREDLVGAARGWQDGPAFRLEYDVRLPGENGEPGMKVRFRDVMVKTADGRVLNHA
                     SVGKWGVRVASVELVIEQAP"
     gene            9615..10403
                     /locus_tag="PLW13_10225"
     CDS             9615..10403
                     /locus_tag="PLW13_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQY70797.1"
                     /translation="MQRRCFVFLLGGAAVAALTGTSGCSASRIGEAVALSQRSEPLQQ
                     APPDAVQRLLIVGDSTGVGTGASSAQGSLAGLIARDHPRLHIDNRSRDGATFADVVQQ
                     LEGEERHDVILVLAGGNDVTRMRALDAVRTDIDRVASMASARATYVVLMPAGNVGNAR
                     FFVPPVSWLMTSRSRTLHDLVRAAAMRYQLAYVNLFMEPEADPFARAPHLSAADGLHP
                     SDAGYLVWYRELLAQTDLGDRLSISRDGAESATVPRQAAPPRRT"
     gene            complement(10461..>10879)
                     /locus_tag="PLW13_10230"
     CDS             complement(10461..>10879)
                     /locus_tag="PLW13_10230"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQY70798.1"
                     /translation="APLPAAELAQSAYAARFREAMDDDFNTPGALALMHEIARELNTA
                     AREGDAARARALAAQLRGFGAILGILGDDPLAFRRGGGGDASEDAGIDALVAERVQAK
                     KAKDFARADAIREELRQRGIVIEDTREGSVWRRETV"
BASE COUNT         1570 a         3906 c         3778 g         1625 t
ORIGIN      
        1 tcgaggctga cgcgcaggtg gtaatagccg accaggtagc ggctgcctgt ctcgccgccg
       61 gaggcacgcg gcccggcacc gcgttgcatg ccggccgtca cgaacacggg taccgtcaga
      121 tcgctgaagc gctccagccc cgcccgtcgc tcgtcggtgg caacgccctg atcgaatgcg
      181 gccacgccgc ggcgcgcgcg cccgaagtcc gcctcgggat acccgtcggc atcctcgccc
      241 cgtcgcgcca cgatttccct gccgtcggcg tcgcggaccg ccgcatactt cacgcccggc
      301 agggccatga aacgcgccag ccgttcactg atcagcgcga tgtcacgctc ctgcaccgcc
      361 cgatcggtgc gcgcgtccgc ctcggccagc agcagcgcat gcgcgaacac cccatccctg
      421 gcggcacgat attcgcgcca tatcatggcg gcggcggcgg ccagcgcccc cgccatggcc
      481 agcgcgacca tgaacaggga gagcctctgg gctatggaac cgcgcaattt catccgctga
      541 gcacccgcat cgctgttacc gggcaccgtc gccccaccca cgaggataga tcaggaatcg
      601 gataacgccg cgccgcggga ggatgttcgg gccagcagga tgacagaggc cgcgagatag
      661 gcgatcagca ccgcggcaac aggccaggcc gccacgccgg tgacggccaa gcccagcacc
      721 gccgccagcg tgaccccgag gcgcagccgc tccagcgccc tgccccgtgg cagctgttcg
      781 agcatgaccc cgatggcaac cagcccgaac agcaggcagg cgaacagcgc cgcgcgtccg
      841 gtcgcgtgca tggccggtgc ggcctgctcc agccacagcg cgaaggccat cagggcgagg
      901 aactgcagcc ccgcgtacca cgccgcgcga ggcgccagcg gcgggtcata gcggcgaaac
      961 tccgcgagcg ggcgcttcac caccgggaag cgcgccgcga catcgtcggg gcgccagccg
     1021 gtgcgcgcga gcagcacgcg cagcttgtcg ctccagcgcc gcgtgtacca cgcatcgcgc
     1081 agcatctggg cgtagatgtg caggttcgcc cacagcgggt tccagctctt cagcgggccg
     1141 aggatgccgt agacggcggg ctcggcctcg cgctcctcct cgaaggtgcc gaacagacgg
     1201 tcccacacga tcagcagccc gccgtagttc ttgtcgacgt aggcgggatt ctgggcatgg
     1261 tgcacgcggt gattcgaagc agtgaccagc acccactcga gcgggcccag cttcgggatg
     1321 tggcgcgagt ggatccagaa ctgatagatc aggtgcgccg aggccaccgt gacgaacacc
     1381 gccgccggca cgcccgcgac gaagcacggg atgtagaaca cccagcccac gaggaagccg
     1441 gtgctggtct ggcgcagcgc cgtggtgagg ttgaactcct cgctctggtg gtgcaccacg
     1501 tgcgcggccc acaacagcgt gcgctcgtgg ctgatgcggt ggaaccagta gtagcagaaa
     1561 tcgtagagca gcagcgccag cagccaggtc cacggcgact gcgcatcgag ccgcgccagc
     1621 gcccagtgct gctcggcctg cgtgaacacc cgcccgccga tatcgaggcc gagcagcttg
     1681 atcgaggtgc tgaggacgcc gcagctcagg ctgttgatgc tgtcgttgag gcggtaggtg
     1741 tcgttgccgc gcgcgcggcc ccacgcgtac tcgaggccga tcgcgagcag gaaaaacggt
     1801 accgcgtagg gaaccaggtc catcggggct gtttccgccg aagtgagacc acgccattct
     1861 agcagcggac gccgccgcgc acgttgatcg gcgcgccgcg accggctaag gtcgcgcccg
     1921 atatgcaagc ccatgccccc cacccgcgcg aacccctgag ccgcgaactg ctgctgatcg
     1981 gcggcggcca cagccacgtg gccgtggtgc gcgccctcgg catgcgcccg gtgccgggcc
     2041 tgcgcgtcac ggtggtgagt cgcgacgtgc acacgcccta ctccggcatg ctgcccgggc
     2101 tggtggccgg ccattacgga ttcgacgact gccacatcga cctgcgcccg ctgtgcgagg
     2161 ccagcggggt gcggctgatc cacggcgagg tcgatgcgct ggaccccgcg cgccagagcg
     2221 tgcgcatcgc cgggcgcgaa tcgctgcgct acgactgggc ctcgatcaac atcggctcgc
     2281 gcccggcgct gggaggcatc gagggcgcca cggcgcacgg catcagcgtg aagccgatcg
     2341 accgcttcct cgcgcactgg ctggcgctgc tcgaggatct gcgcgccaac ccgcgtccct
     2401 gccggctgct catcgtgggc ggcggcgccg ccggggtgga aatcgcgctg gcctgcgcct
     2461 ggcgcgcgcg ccaggcgctc ggcgcgcaag ccgcgctgtt cagcgtgggc atcgtcaacg
     2521 ccgcccccgt gctgctgccc tcgcacaacg cgctggtgcg ccgctacttt cgccgcgaac
     2581 tcgaacgccg cggcatcgtg ctgcactcgg gcgcgcgtgt cgcggcggtc gacgcgcagg
     2641 gcgtgatgct cgcggacggt cggcgcctgc aagccgagca gctggtctgg gccacgcagg
     2701 ccggcgcccc cgcgtggccc gccgcggccg ggctcgcctg cgatgccaac ggcttcgtgc
     2761 gcgtcgacgc atgcctgcgc agcctgtcgc acccgaacgt cttcgccgcc ggcgatatcg
     2821 cggccctgcc cgaaccctgc cccaagagcg gcgtctacgc ggtgcgcgaa ggccaggcgc
     2881 tggcgctcgg cctgcgccgc gtcgcgctgg gcggggaacc cgtggtctat cgcccgcaac
     2941 ggcgcttcct cagcctggtg agcacggggg atcgccgcgc cgtggtctcg cgcggcccgc
     3001 tgttcgccgc cggcgcctgg gcctggcgct ggaaggaccg catcgatcgt gccttcatgg
     3061 cgatgtaccg cgtggagccc gccgcgccgc ccgagcccgg cgaggcgccc gcgatgcgct
     3121 gcggcggctg tgccgcgaag atcggcggcg acgcgctggg gcgcgtgctg gcggatctcg
     3181 cgcgcccgcc gcgcgagggc gtcgaggccg gcatggacat cggtgacgac gccgcggtgc
     3241 tcgccccccc gcccggacag cgcctgatcc agagcgtcga ctacttcagg ggcttcatcg
     3301 acgacccgtt cctggtcggg caggtggcgg cggtgcacgc gctcggcgac ctgctcgcga
     3361 tgggcgcgcg cccgcactcg gcgctggcga tcgcgaccgt gccctacgcc gcagcgcgcg
     3421 tcatggagca gacgctcggc cagctgatgc agggggcgct gaaaacgctg gatgccgagg
     3481 gtgtcgcgct ggttggcggg cacagcagcg agggcgccga gctcgccttc gggctcagcg
     3541 tgaacggcta cgccgcgccg ggcgaaatcc tctacaagag cgggctgcgc gacgggcacg
     3601 cgctggtgct caccaaggcc atcggcaccg gcacgctgct ggcggccaac ggcgccgcgg
     3661 cggccgtggg gcgctggatc gacggcgcgc tggccggcat gacgcagtcc tcgcgcgcgg
     3721 cggcggacat cctgcgccgg cacggggccg ccgcctgcac cgacatcacc ggcttcgggc
     3781 tgctcgggca cctgctggag atgctgcgcg cctcgggcac cggggcgcgg ctgagcatcg
     3841 acgccgtacc ggtgctggag ggcgcgcgcg agtgcctggc gcgcggctac cgcagctcgc
     3901 tgcacgagag caaccgcgcc aacctggcgg cgctggtgca cccgctgagc gcagcgcccg
     3961 cgcaggcgct cgaagtcctg ctcgacccgc agacggcggg cgggctgctg gcggggattc
     4021 cgcctgcccg ggcgcagagt tgcgtcgccg agctgcgcgc ggcgggctac cccgcctgcg
     4081 ccgtgatcgg cagctgcagc gcggcggcgg gtccaggcgg cgtcctcatc gactgaggca
     4141 ccattcacgc acggcggcga aatcgccgcg aaacaccacg cgtccggcgc tcttgccgag
     4201 ctggtactcg tacatcgggt cgtagtaatc gcgcagcagc agttcgatcc aggcgcggtg
     4261 cccggagggg tcgccacgcg tcccctgctg gtgcagcgcg gcgcgcatca acccctcggc
     4321 catggcgtaa cgctcgccgc cgaggcggcg ctggatgcgc tgcaggctct cgtgcaaggc
     4381 ggcgccgaac tgcgcgaagc cgtgctccgg gtccagcgcc acgcgctcgc gcaggtcctc
     4441 gcagatgtag tcgcgcagga tctgctccac gcgcgaggcg aagggcgcgt cgatgatcac
     4501 cagcggcgcg gccttcatgg cctggtacag ttcgagcggc acgctgatcg agccgatcgt
     4561 gcgactctcg tcctcgagga acaacgccgc cccgggcgcg gcatggcgcc ggcgcagcag
     4621 caacagcgcg agccggttct cgaagtcggt ctgcgccggc gtcgcgccca cgcggcgccc
     4681 gaaactcgag ccgcggtgat gcgccagttc ctccaggtcg acgccccagg gcagcgcctt
     4741 gacgaggtcc gtcttcgccg accccgtgcg cccgccgatc acgtacagcg gttcgcgcgg
     4801 ctccggcgcc tccagttcgc gcaacagccg gtgccgcagg gccttgtaac cgccctgcac
     4861 caggcgcact gccaccccgg cctcggcgat ccactgcgta gccagccccg agcgcatccc
     4921 accgcgccag cagtggacca gcgcatccgg atgctcctgc agcaccgcgc accagcgcgc
     4981 gatgcgctcc tccctggtgc tgccgctcac cagccggtgg cccagcgcga cggccgcctc
     5041 ctgccccttg cgcttgtacg tggtgcccac ctgctcgcgc tcggtggtgg tgaggatcgg
     5101 caggttcacg gcgcccggga tcgcgccgcg ggcgaactcg atctcggcgc gcacgtcgat
     5161 gaagtgcgcg ccctcgcgca gcagcgtgtc gaattgttcc gggccgacga tttcggggcg
     5221 catgccgggt cctgggtcgg atggcggggc tgcgatgcta accgcgccgc ccccgccctg
     5281 ccagcgccgc tggcggaacg gggatatatg acaatccgta cactttgcga atgattcgca
     5341 ttttccgcta gactccgcgc atggaaaaca gcaataccag tttttcccgg ctgcagcgtg
     5401 gcgaccgtgc gcgcgtgctg ggctttggcc ccatgcccga gtactaccgc aaccggctgc
     5461 tcagcctggg cctgaccccc ggcaccgagt tcacggtcgc acgcgaggcg ccgctaggcg
     5521 acccggtgca gatcaacctg cgcggtttcc gcctgagcgt gcgccgcgcc gaggccgcgg
     5581 gcctggaggt ggaactactg tgaacaagcc cttcacgatc gcgctggtcg gcaaccccaa
     5641 ctgcggcaag accacgctgt tcaaccgcct gaccggcacc caccagagcg tgggcaactg
     5701 gcccggcgtg actgtcgagc gcaagaccgg cagcttcacg cacgagggcc gccagttcct
     5761 ggtggtcgac ctccccggca ccttctcgct gcacgtctcg cgcgacgacg acgcgatcga
     5821 tcagcagatt gcccaggcct acgtgctggc gcgcgaggcc gacctgatcg tcaacatcat
     5881 cgacgcctcg agcatcgcgc gcgggctgta cctgagctcg cagctgttcg atgccagcgt
     5941 gcccgtggtg gtggcgctga acatggtcga cgtcgcgcag tcgcagggcg tgcacgtgga
     6001 cgcgcaccgc ctcgcggcct gcctcggctg ccccgtggtg ccgctggtcg cgagccgcgg
     6061 cgagggcgtg cgcgcactga tcgaggaagt gtgcgcggga ctgcagaccc ggcagccgcc
     6121 gccgcagcgc atggcgctgg acgccacgct cgaatcggca ctgctgcgcg tgcaggccgc
     6181 gctcgatccc gcggatgcgg gcccggccgg acgcatgctc gccggagcct tgctcgcgcg
     6241 cgacgccgcc gtgctggaac gcatcggcga ggccgcacga gcgcgcgtgc tcgcgatcgt
     6301 cgacgagcta gaggccacgc tcggtggcaa cgcgggcgat gcgctgatcg gcgcgcgcta
     6361 ccaggccatc ggcaccatcg tggaccaggc ggtgcgcacc gaggacgcca agcgcttcaa
     6421 cctcacggat gcgctcgatc gcgtgctgtt gaaccgcgtg ctcgctttcc cgctgctgct
     6481 cggcgtgatg tacctgatgt tcatgttcac gatcaacgtg ggcggggcct tcatcgattt
     6541 cttcgatatc gccgccggcg cgctgttcgt cgaggcgccg cgcctggcgc tggatgcgct
     6601 cggcctgccg gcctggctcg tgaccgccgt cgccgatggc gtcggcggcg gcgtgcaact
     6661 ggtcgcgagc ttcatcccga tcatcgccac gctgttcctg ttccagagtt tcctcgagga
     6721 ctcgggctac atggcgcgcg cggccttcat cctcgatcgc ctgatgcgcg cgatcggcct
     6781 gccgggcaag tccttcgtgc cgctgatcgt cggcttcggt tgcaacgtgc ccgcggtgat
     6841 ggccacgcgc agcctcgaca cccagcagga ccgcctgctc accgcgatga tggcgccgtt
     6901 catgtcctgc ggcgcgcggc tcaccgtgta tgcgctgttc gccgccgcgc tgttccagcg
     6961 caacggccag aacgtggtgt tcgcgctgta cctgatcggc atcgcgctgg cgatcgtgac
     7021 cgggctgctg gtgcgccggc gcctgctggc gcgcgatctg tcgccgttcc tcctcgaact
     7081 gccgccctac catgtgccga cactgcgcgg gctgctcacc cacacctggt ttcgcctgca
     7141 gggcttcgtg ctgcgcgccg gcaaggcgat cgtggcggtg gtgctgctgc tgaacttcgt
     7201 gaactccatc ggcaccgacg gttccttcgg caaccagaac accgagcgct cgctgctgtc
     7261 ggcgatcggc aagagcatca cgccggtctt cgcgccgatg gggctgaccg aggccaactg
     7321 gccggcgacg gtcggcatct tcagcggcgt gttcgccaag gaagtggtgg tgggcacgct
     7381 ggacgcgctc tacaccaaca tggcgcgcgc ggatgacgtg gcgccggagc ccgtgagcat
     7441 cggcgcgatg ctcgaggagg ccgtggcctc ggtgggcacc aaccttgccg cgctcggcga
     7501 caccttcggc gatccgctgg gcatgggcaa cctcgacgac agcgccgcca tcgccgagga
     7561 acaggcggtg cattcgggca cgctcggcct gatggccacg ctgttcgacg gccagctcgg
     7621 cgcgttcagc tacgtgctgt tcgtgctgct gtacatgccc tgcgtggcca cactgggcgc
     7681 gatctacaag gagctcgggg gtttctgggc gttcttttca gcgacctgga acacggtgat
     7741 cgcctacacg ctggcggtga ccgtctacca ggccggcacc ttcgccgcgc atccggtttc
     7801 atccgcggcg tggatcgccg gttgcgccgc ggcgctgctc ggctgctatg cgtggctcat
     7861 gcacctgggg cgccagcgcg cgggcagtgg caagctgatc ccggtggtga acctgtaacg
     7921 tgggtccgca ttcatgcgga catggtccgg ctgccgaacg cgcatccgaa cagggattca
     7981 gacccacggc gcggcgccga ttgcgccggc acgcgcagcg acaggatgcg cagccgatag
     8041 tcgggatcca cgccgaggac gctggcatcg ggcagcagac gccacggctc ctccggtgta
     8101 accggctcgg aggccacctc caccgcgcgg taagcggctc ccggttcgtg gtgcacgtgc
     8161 gcccgcccgc agacggaaca ccgcacgatc gcgtcgcgac agaggtacca caggctgcgg
     8221 ttcagccgcg aggccaccat ctgccggcca tcgctcagca cgatgttcag cccgaccttg
     8281 ctgccgggag cgatctcgtg acaccacgcc agcacgcgct ccaggcccgc acgcaccgtg
     8341 ccgggcagat cgtcctgcgg tccgcgcgac cagcgcgtga gcaggtagtg gaacaggtgc
     8401 tcgctgtcgg tctggccgcg gatcgcggcg cggtgcagcg gatcggtctc cgcgagcatg
     8461 cgctggcgca cctgcccgaa gtccgggatc gtcccgttgt gcgcgaaggt gaagcgcccg
     8521 tggcggaaag gatgcgtgtt ctcgacgctg gtctggccca ccgttgcgcg ccgcacatgg
     8581 gcaacgaccg catgcgcgtg cacgcgtgcg gccttcctcg ggaaatgctc gccgtggtag
     8641 gccgcccagg cctgcttctc gacactcggc gcgccatccg tatagtccgc cacgccccag
     8701 ccatgcccgt gcacgactcc ctcggcatcc ccctcgctct ggcgcatcag gctgttctgc
     8761 gagcgcacga ggctgcattc cgcacgcgtg ggttcgtttg ccatcagcgc gtaaagccgg
     8821 cacatgtcga ggcgctctcg gggatgaact tatcctgagt aagagcacag atccggcttc
     8881 atgcaaggcc gatgtgtgat tttcatgggc tttccgcata atttacgcca catcggcagc
     8941 tgccgacgcg gggtagccac ggctggtcca tacggtgacg gttcgcgtag aaccggcgta
     9001 cccccggaga actgccatgc cccgcccctt cctgccagtc atcgccagtg tcgcgtccct
     9061 gctgctgctc ggcggctgcg cccgcgcgcc cgggttgccg caagagaccg ccccctcggc
     9121 gttcgtcatc gagcgcgatc tcgagggcgc cacggtcgcg cggggcgagt tctcggcgat
     9181 caacggcgtc aggcgcggct tcaccgtcta cctcgaaggc gtgcgctccg gcgacacgtt
     9241 cactttgaag gagcgtttcg agtacgacga cggagagcgc gaccacaaga cctgggtgct
     9301 gaggctcgcg ggagacggac aatacaccgg gacgcgcgaa gatctcgtgg gagcggcgcg
     9361 aggctggcag gacggcccgg cctttcgcct cgaatacgat gtgcgcctgc cgggcgaaaa
     9421 cggcgagccc ggcatgaagg tgcgtttccg ggacgtgatg gtgaagacgg ccgacgggcg
     9481 cgtcctgaac cacgcgtcgg tcggaaaatg gggtgtgcgc gttgcctcgg tggaactcgt
     9541 catcgagcag gcgccatgaa cgtcgtcccc cgcaagaatt cctgaagcgt caccttcccg
     9601 gctgcgcaat ggccatgcaa cggcgctgct tcgtgttcct cctcggggga gcagctgtcg
     9661 ccgcgctgac tggcacatcc ggttgttcgg cctcgcgcat cggcgaagcc gtggcgttgt
     9721 cgcagcgcag cgagccgctg caacaggcac cgcccgacgc cgtgcaacgg ctgctgatcg
     9781 tgggcgacag caccggggtg ggcaccggcg ccagctcggc gcagggcagc ctggccgggc
     9841 tgatcgcacg cgaccaccca cgcctgcaca tcgacaaccg ctcccgcgac ggcgccacct
     9901 ttgccgacgt cgtgcagcag ctggagggcg aggagcgcca cgacgtgatc ctggtgctgg
     9961 ccggaggcaa cgacgtcacc cgcatgcgcg cgctcgatgc ggtgcgcacc gacatcgacc
    10021 gcgtggcgtc gatggccagc gcccgcgcca cgtacgtggt gctgatgcca gccggcaacg
    10081 tgggcaatgc gcggttcttc gtgccgccgg tctcgtggct gatgacttcg cgctcgcgca
    10141 ccctgcacga tctggttcgc gccgcggcca tgcgctatca gcttgcctac gtgaacctgt
    10201 ttatggagcc cgaagcggat cccttcgcga gggctccgca cctgagcgca gcggatggtt
    10261 tgcaccccag cgacgcgggc tacctggtgt ggtaccgcga gttgctggcg cagacggatc
    10321 tcggcgaccg gctgtcgata tcgcgcgacg gcgctgaatc ggcgacggtg ccgcgccagg
    10381 cagcgccgcc gcggcggacg taacggacaa aacatgaatg cgcgggtagc gggcggggcc
    10441 cgctcccgca ccagtgccgc tcacaccgtc tcgcggcgcc agaccgagcc ttcgcgggtg
    10501 tcctcgatca cgatgccgcg ctggcgcagt tcctcgcgga tggcgtcggc gcgcgcgaag
    10561 tccttcgctt tcttggcctg cacgcgctcg gccaccagcg catcgatccc ggcgtcctcg
    10621 ctcgcatcgc caccgccgcc gcgacggaag gccagcgggt cgtcaccgag aatcccgagg
    10681 atggcgccga agccgcgcag ttgcgccgcc agcgcgcggg cccgtgccgc atcgccctcg
    10741 cgcgccgcgg tattgagctc gcgcgcgatc tcgtgcatca gggccagcgc acccggcgtg
    10801 ttgaagtcgt cgtccatcgc ctcgcgaaag cgcgcggcgt acgcactctg cgccagctcc
    10861 gcggccggca gcggcgcca
//
LOCUS       DAORUQ010000107        10828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6334_length_10828_cov_5.420960, whole genome shotgun sequence.
ACCESSION   DAORUQ010000107 DAORUQ010000000
VERSION     DAORUQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_10828_cov_5.420960"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="PLW13_10235"
     CDS             complement(<1..1754)
                     /locus_tag="PLW13_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70799.1"
                     /translation="MQKPIPGGRAALLLALLPAAPGIAAQLETVVVTGSRAPQPLSEL
                     AASIRAVGSEELQLVRAVHISEALSRVPGTWISRGNGQEHLTAIRSPVFTGPGSCGAF
                     YLAEDGLRLRPAGTCNVNELSEVNSEQAARIEVLRGPGTAVHGGNAQHGVINVISAPP
                     PEARDASLTLTGGPNGYARALGSYGERTDNGAWRLSLNTTHDGGYKDDSGYDLQKLNY
                     RHDAEWSGVTLQSLLSLGNLEQETAGFVIGQDAYEDSGRQKENPNPEAFRDNQVARWY
                     GRFSLDAGPGVLSVTPFYRYQDMRFLQHFLLGQPLEENGFKSSGWQSEWRVALDDALE
                     LVTGLDGEWSTSFLQETQRDAVPGNSVLPAGKHYDYEVEGLNGALFAQLAWTPLAATR
                     IDAGLRLEHQRYDYDNRMLDGATREDGTPCGRPGAPVPCRYSRPADREDEFTEPALNL
                     GLVQDIGGGQELVLSYAHGFRPPQSAELYRLQAGQLVADIDSEQVDSIEAGWRGTRET
                     LSWQLAAYYMQKENIIFQDAERRNVGDARSEHRGIEYSVDWRFAEQWALAADGTYARH
                     RYDGNAPLQGLPAGTGIDG"
     gene            complement(1779..>2025)
                     /locus_tag="PLW13_10240"
     CDS             complement(1779..>2025)
                     /locus_tag="PLW13_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70800.1"
                     /translation="RYHETRPEYRSAEVPVIRLLIVLAVVILMAFYMNYNSPRGGGQA
                     TSPEQQYEQATGEAQALEQQMQQQAQSQLDAIDAGTQ"
     assembly_gap    2026..2125
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2129..3628)
                     /locus_tag="PLW13_10245"
     CDS             complement(2129..3628)
                     /locus_tag="PLW13_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQY70801.1"
                     /translation="MDDNTLLIGAAEQPVRLPLCIANRHGLVAGATGTGKTITVQGLA
                     EGFSAAGVPVFVADVKGDLAGLAQPGEPSERIAKRWQKMGLPQPSFAGAPVALWDVYG
                     EAGTPLRLSISELGPQMLARMLELNETQESVLSLAFRFADDEGLLLLDMADLNTTLGH
                     LLEHADELGAAYAAFSRPSVAAIQRRMMLLENAGGAGFFGEPAVRLEDLMRTARDGRG
                     VVNLLDARKLINQPRLYTTFLLWLMSELFEDLPEIGDPERPRMVFFFDEAHLLFDGAA
                     RALSDRVEQVVRLIRSKGVGIYFISQSPGDIPDDVLAQLGNRVQHALRAYTPAEQKAV
                     RTAADSFRVNPAFDTRAVIGELGVGEALVSTLDEHGVPMIVQRALVRPPASRMGPLSE
                     AERQALLAACPMHARYAKTQDPVSAHEILQQRAGERLQREEREAREAAEQKEELARER
                     EQRRREPAPRRSNRQSTTEALVKSVARTVGTGLGRSILRGILGSMTRRR"
     gene            complement(3634..5232)
                     /locus_tag="PLW13_10250"
     CDS             complement(3634..5232)
                     /locus_tag="PLW13_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY70802.1"
                     /translation="MPRILYPTLLALLLTALATAPLRADDALARALAQLAQHGELRVG
                     AARVRDSALLHEFYVLRAQQPAWTDPAARAHLLAAVQGVESEGLRPADYHLAALLAAP
                     PDEPAEAPGMELLRSDALIRLAAHHLGGKLDPLSVEAEIDLHSPELGAAAIAQLAAAV
                     DSGDIAGTLQRLAPQSPVYQGLKRGLAEYRGIAARGGWPQIPEGPTLRPGEHSPRVAL
                     LRQRLLASGDLAADTGADPQFYDPALEAAVRHFQARHLLAADAAVGKRTLAAANVSVD
                     ARIDQIRVNLERARWLLHDLPPTHVLVDIAGFEVRYVREGVELLRSRAAVGRPYRKTP
                     IFRSAIRYLEFNPTWTVPPTILTNDVLPEIRKNLGYLAKRHMRVLGADGREVDPASVD
                     WRRYSGKNLPWVIRQDPGPENALGQVKFMFPNAHAVYLHDTPERKVFLQAERAFSSGC
                     IRIEEARRLALLLLEAEGRSEAEVEAKFADLKTQRVALARPVPILLYYWTVAIEDDGD
                     VLFKRDLYDRDAELLAALDTTDGD"
     gene            5353..5940
                     /locus_tag="PLW13_10255"
     CDS             5353..5940
                     /locus_tag="PLW13_10255"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HQY70803.1"
                     /translation="MQYPTDPSAFPRPDSRRGFLKRLAAAGALGAMGTRALAALPGPV
                     STPAPVRELGFLNLHTGEKLRVPYWENGRYLQDALAEINHVLRDHRANAVHAIDTGLL
                     DLLARLQAAVGNSRAFEVISGYRAPTSNEMLRARSSGVAQGSLHLQGKAIDIRLPGSD
                     LLRLHQAAVGLKGGGVGLYRASNFIHVDTGRVRYW"
     gene            complement(5960..6904)
                     /locus_tag="PLW13_10260"
     CDS             complement(5960..6904)
                     /locus_tag="PLW13_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70804.1"
                     /translation="MAEHLLIPKRLARALPGLKRAAWWLEAAFIRGVIGLLRALSPER
                     ATALAMRVTGVLGPLLPMWDKVARNLAIAFPALPADERARLRRRIFAHLGAAIAELVQ
                     SERIWAERERRLEFRADPSIRGLQEGGRPMVLVTAHVGAWQLSNLVGAHFGLPITSLY
                     ASESNPLIAAQVLQLRERLRVRWIPSTGGIRSLITELRAGHSVGLACDTRLDQGEAVP
                     YFGHAAMTNTVPARLALKQDCELVPVRVERLGGARYRVTMGAPIRPRDAGAAQAAQAL
                     DMSAQLNALFESWIRETPGEWMCLARRFPKELDKAARG"
     gene            complement(6927..7565)
                     /locus_tag="PLW13_10265"
     CDS             complement(6927..7565)
                     /locus_tag="PLW13_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQY70805.1"
                     /translation="MADNTRTLQQEPSIRLFRNDFLEALTHVHPIVPLLFWSPVVAWL
                     LWRSVFVHELPAAGLLGVALAGLIVWTLSEYLLHRFVFHYPATGRVGKYLVFMFHGVH
                     HDAPRDKTRLVMPPAGAIIVMFLLWQVFRLVVPAPWIEPFCAFFIVGYLVYDYIHYAT
                     HHFAMKHPWLHYLKVYHLQHHYGAEGKRFGVSSPLWDKVFGTFPADGVARRA"
     gene            complement(7558..8580)
                     /locus_tag="PLW13_10270"
     CDS             complement(7558..8580)
                     /locus_tag="PLW13_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY70806.1"
                     /translation="MARFILGHRLRALGERHGAIEAVLYRLDRAFFGTLLWLIRRLPV
                     DAASRLGARIGAALGPRFRKGRALDDNLRLAFPDKSEDERKRIARQAWGNAGAVFAEY
                     AHLDEIAFGENGARIAIEVLGDIRTLRDATQPSVFVSAHQANWEVIAAAIARRNVPLA
                     AVYAPPTNPLLDELLNRWRTRIGCELLARDESMRPMLHALRQGTSVGIVMDRRVDSGK
                     DVALFGHPKCTTLIPARLALRYGFDLVPIRVERLQGARFRVTFHPPVAVPAGGDEIER
                     AIRMTGSVHALFEQWIRARPGDWFPSKRLWPKSAYAVRRGRVQNHAAPTPPAANPSPA
                     RELTDG"
     gene            8769..10052
                     /locus_tag="PLW13_10275"
     CDS             8769..10052
                     /locus_tag="PLW13_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HQY70807.1"
                     /translation="MIEIDIGVAQRFLVALLIGALVGVEREKSQSASGHRSIAGLRTF
                     ILLALLGSVSAWIALHVGGALVFAITLGAVALAVVTGYVLEIRAQPDKLGLSSEFAAI
                     AVFLLGGVVMYGYPELAVALGIVTSAVLAFKQPLHGMVARVGLDDIYAVLKLLIASFI
                     VLPLLPNHAVDPWGALNPYTLWLLVILISGLSLVGYVAVRLLGEAHGTVLTGFAGGLV
                     SSTAVSLSFARQSRTDPGPVAADALAAGILTAWAVMFVRVMITVAIVNRELLPTLVRG
                     FGVMALAAAGMALAFYVRGLRRASVTARGDSLPVKNPFSLGSAVQFALLFAVVLVIVE
                     LTQKYAPVEGLYVVAAVAGLTDVDAITLSMAQGSRGGIGFAVATHAIGIAAVSNTLVK
                     CTMVLALGRGPLRPRLAVATAVLVACGGLAALIVN"
     gene            complement(10149..10828)
                     /locus_tag="PLW13_10280"
                     /pseudo
     CDS             complement(10149..10828)
                     /locus_tag="PLW13_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009314343.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
BASE COUNT         1455 a         3632 c         3941 g         1700 t
ORIGIN      
        1 ccgtcgatgc cggtgccggc gggcagcccc tgcaacggcg cgttaccgtc gtagcggtgc
       61 cgggcatagg tgccgtcggc cgcgagcgcc cactgctcgg cgaagcgcca gtccacggag
      121 tactcgatgc cgcggtgctc gctgcgcgca tcgcccacgt tgcgccgctc ggcatcctgg
      181 aaaatgatgt tctccttctg catgtagtag gcggcgagct gccacgagag ggtctcgcgc
      241 gtgccgcgcc agccggcctc gatgctgtcg acctgttccg agtcgatgtc cgcgacgagc
      301 tgcccggcct gcaggcggta gagctcggcg gactgcggcg ggcggaagcc gtgcgcatac
      361 gacagcacca gttcctgccc gccgccgatg tcctgcacca gcccgaggtt cagtgccggc
      421 tcggtgaatt cgtcctcgcg atccgccggg cgcgagtagc ggcagggaac cggcgcgccg
      481 gggcgcccgc agggcgtgcc gtcctcgcgc gtcgcgccat cgagcatgcg gttgtcgtag
      541 tcgtagcgct ggtgctccag gcgcaggccc gcgtcgatgc gtgtggccgc cagcggcgtc
      601 cacgcgagct gcgcgaacag cgcgccgttc aggccctcga cctcgtagtc gtagtgcttg
      661 cccgccggca gcacgctgtt gcccggcacc gcgtcgcgct gcgtttcctg caggaagctc
      721 gtgctccact cgccgtcgag gccggtcacg agctcgagcg catcgtccag cgccacccgc
      781 cattcgctct gccagccgct gctcttgaag ccgttttcct cgagcggctg cccgagcagg
      841 aaatgctgca ggaagcgcat gtcctggtag cggtagaacg gcgtcacgct gagcacgccg
      901 ggcccggcgt ccagggagaa gcgcccgtac cagcgcgcga cctggttgtc gcgaaaggcc
      961 tccgggttgg ggttttcctt ctgccggccg gagtcctcgt aggcgtcctg gccgatcacg
     1021 aagccggcgg tttcctgctc caggttgccg aggctgagca gcgattgcag cgtgacgccg
     1081 gaccattcgg cgtcgtggcg gtagttcagc ttctgcaggt cgtagcccga gtcgtccttg
     1141 tagccgccgt cgtgcgtggt gttcaggctg agacgccagg cgccgttgtc cgtgcgctcg
     1201 ccgtagctgc cgagcgcgcg cgcgtagccg ttgggcccgc ccgtcagcgt gaggctggca
     1261 tcgcgcgcct cgggcggcgg cgcgctgatc acgttgatca cgccgtgctg cgcgttgccc
     1321 ccgtgcaccg cggtgcccgg gccgcgcagc acctcgatgc gcgcggcctg ctcgctgttg
     1381 acctcggaca gttcgttgac gttgcaggtg ccggccgggc gcaggcgcag gccgtcttcg
     1441 gcgagataga acgcgccgca gctgccgggg ccggtgaaca ccggcgagcg gatcgcggtg
     1501 aggtgctcct ggccgttgcc gcggctgatc caggtgccgg gcacgcgcga gagcgcctcg
     1561 ctgatgtgca ctgcgcgcac cagttgcagt tcctcgctgc cgacggcgcg gatggatgcc
     1621 gcgagctcgg agagcggttg cggcgcgcgg ctgccggtga ccaccacggt ctcgagctgg
     1681 gcagcgatgc cgggcgcggc gggaaggagg gcgagcagca gcgcggcgcg cccgcccggg
     1741 atcggtttct gcatgggagc cttgtcgtgg tcttgtgttt actgcgtgcc ggcgtcgatg
     1801 gcatcgagct ggctctgcgc ctgctgctgc atctgctgtt ccagcgcctg ggcttcgccc
     1861 gttgcctgtt cgtactgctg ctcggggctg gtggcctggc caccgccgcg cgggctgttg
     1921 tagttcatgt agaaggccat caggatcacg acggcgagca cgatcagcag gcgtatcacg
     1981 ggaacctccg cggaacggta ttcggggcgc gtttcatgat agcgcnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnngcgct agcgccggcg cgtcatcgag ccgagtatgc
     2161 cgcgcaggat gctgcgcccg agacccgtgc ccacggtgcg cgcgacgctt ttgaccagcg
     2221 cctcggtggt gctctggcgg ttcgaacggc gcggtgccgg ctcgcggcgg cgttgctcgc
     2281 gctcgcgcgc gagttcctct ttctgttccg cggcctcgcg cgcctcgcgc tcctcgcgct
     2341 gcagccgctc gccggcgcgc tgctgcagga tctcgtgcgc cgagaccgga tcctgcgtct
     2401 tggcgtagcg cgcgtgcatc gggcaggcgg ccagcagcgc ctggcgctcg gcctcgctca
     2461 gcgggcccat gcgcgaagcg ggcgggcgca ccagcgcgcg ctgcacgatc atcggcaccc
     2521 cgtgctcgtc gagcgtcgag accagcgcct cgcccacgcc gagctcgccg atcaccgcgc
     2581 gggtgtcgaa ggccgggttc acgcggaagg aatcggccgc ggtgcgcact gccttctgct
     2641 cggcgggggt gtaggcgcgc agcgcgtgct gcacgcggtt gcccagctgc gccagcacgt
     2701 cgtcggggat gtcgcccggg ctctggctga tgaaatagat gcccacgccc ttggagcgga
     2761 tcaggcgcac gacctgctcg acgcgatcac tgagggcgcg cgccgcgccg tcgaacagca
     2821 ggtgcgcctc gtcgaagaag aacaccatcc gcggccgctc gggatcgccg atctcgggca
     2881 gatcctcgaa cagttccgac atcagccaca gcaggaaggt ggtgtagagg cgcggctggt
     2941 tgatgagctt gcgcgcatcg agcaggttga ccacgccgcg gccgtcgcgc gcggtgcgca
     3001 tcaggtcctc gagacgcacc gcgggctcgc cgaagaagcc ggcgccgccg gcgttctcca
     3061 gcagcatcat gcgccgctgg atcgcggcga ccgaggggcg cgagaaggcg gcgtaggcgg
     3121 cgcccagctc gtcggcatgc tccagcagat ggcccagcgt ggtgttgaga tccgccatgt
     3181 cgagcagcag caggccctcg tcgtcggcga agcgaaacgc cagcgacagc accgattcct
     3241 gggtctcgtt gagctcgagc atgcgcgcga gcatctgcgg ccccagctcc gagatgctca
     3301 ggcgcagcgg cgtgccggcc tcgccgtaga cgtcccacag cgccaccggc gcgccggcga
     3361 aggacggttg cggcaggccc atcttctgcc agcgcttggc gatgcgctcg ctcggctcgc
     3421 cgggctgcgc cagccccgcg aggtcgccct tgacgtcggc gacgaacacc ggcacgccgg
     3481 ccgccgagaa gccctcggcg agtccctgca ccgtgatggt cttgccggtg ccggtggcgc
     3541 cggccaccag gccgtggcgg ttggcgatgc acagcggcag ccgcacgggc tgctccgcgg
     3601 cgccgatcag cagggtgttg tcgtccatcg gtctcagtct ccgtcggtgg tgtcgagcgc
     3661 cgcgagcagt tcggcatcgc gatcatacag gtcgcgcttg aagagcacgt cgccatcgtc
     3721 ctcgatcgcg acggtccagt agtagagcag gatcggcacc ggtcgcgcga gcgccacgcg
     3781 ctgtgtcttc aggtcggcga acttcgcctc gacctcggcc tcgctgcgtc cctcggcctc
     3841 gagcagcagc agggcgaggc ggcgcgcctc ctcgatgcgg atgcagcccg agctgaatgc
     3901 ccgctcggcc tgcaggaaga ccttgcgctc gggcgtgtcg tgcaggtaga cggcatgggc
     3961 gttggggaac atgaacttca cctgccccag cgcgttctcc ggccccggat cctggcggat
     4021 cacccagggc aggttcttgc cggagtagcg ccgccagtcg acgctggccg gatcgacctc
     4081 gcgcccgtcc gcgcccagca cgcgcatgtg gcgcttggcg agatagccga ggttcttgcg
     4141 tatctccggg agcacgtcgt tggtgaggat ggtgggcggt acggtccagg tggggttgaa
     4201 ttccaggtag cggatggcgg agcggaagat cggggtcttg cggtaggggc gcccgaccgc
     4261 cgcgcggctg cgaagcagtt cgacgccctc gcgcacgtaa cgcacctcga agccggcgat
     4321 gtccaccagc acgtgggtgg gcggcaggtc gtgcagcagc cagcgcgcgc gctcgaggtt
     4381 gacgcggatc tggtcgatgc gcgcgtccac gctcacgttg gccgcggcga gcgtgcgctt
     4441 gcccacggcg gcgtccgccg cgagcaggtg gcgtgcctgg aagtggcgca ccgcggcctc
     4501 cagcgcagga tcgtagaact gcggatcggc gccggtatcc gccgcgaggt cgccgctggc
     4561 gagcaggcgc tggcgcagca gcgccacccg cggcgagtgt tcgccggggc gcagcgtggg
     4621 gccttcggga atttgcggcc agccgccgcg cgcggcgatg ccgcggtact ccgccagccc
     4681 gcgcttcagg ccctggtaga cggggctctg cggggccagg cgctgcagcg tgcccgcgat
     4741 atcgccggag tccaccgctg cggcgagctg cgcgatggca gcggcgccca gttccgggct
     4801 gtgcaggtcg atttccgcct cgacgctgag cggatcgagc ttgccgccca ggtggtgggc
     4861 ggcgaggcgg atcagcgcat cgctgcgcag cagctccatg cccggagcct cggcgggctc
     4921 gtcgggcggt gccgccagca gcgcggcaag gtggtagtcg gcgggccgca gcccctcgct
     4981 ctcgacaccc tgcacggcgg cgagcaggtg tgcgcgcgct gcgggatccg tccacgccgg
     5041 ctgttgcgcc cgcaggacgt agaactcgtg cagcagcgcg ctgtcgcgca cgcgcgcggc
     5101 gccgacgcgc agctccccgt gctgcgcgag ctgcgccagc gcgcgcgcca gggcgtcgtc
     5161 ggcgcgcagc ggggcggttg caagggcagt gagcagcagg gcgagaagcg tcgggtacag
     5221 gatgcgcggc attgagtcaa gctttaccaa tcagtcactt gcggctattt tgacgaagca
     5281 ccctcacgat ccgctggctc cgaccgggtg caccttagca gcttcgctac caataatcgc
     5341 actggagtcg tcatgcagta tccgaccgat ccttctgcgt tcccgcgtcc ggacagccgg
     5401 cgcggattcc tcaagcgcct ggccgcggcc ggcgctctgg gcgcgatggg tacccgtgcc
     5461 ctcgccgcgc tcccgggccc ggtgagcacg ccggcaccgg tgcgcgagct cggtttcctc
     5521 aacctgcaca ccggcgagaa gctgcgcgtg ccctactggg aaaacggccg ctacctgcag
     5581 gacgcgctgg ccgagatcaa ccacgtgttg cgcgatcatc gcgccaacgc ggtgcatgcc
     5641 atcgacaccg ggctgctcga cctgctggcc cggctgcagg ccgcggtcgg caattcgcgc
     5701 gccttcgagg tcatctcggg ctaccgcgcg ccaaccagca acgagatgct gcgcgcgcgc
     5761 agctccggcg tggcgcaggg cagcctgcac ctgcagggca aggccatcga catccgcctg
     5821 ccgggttcgg acctgctgcg cctgcaccag gctgcggtcg ggctgaaagg gggcggcgtg
     5881 gggctctacc gtgcgtcgaa cttcatccac gtcgacaccg gtcgcgtgcg ctactggtga
     5941 tcccccgggc gcgcggcgtt cagccgcgcg ccgccttgtc gagctccttc gggaagcgtc
     6001 gcgcgaggca catccattcg ccgggtgtct cgcgaatcca tgactcgaac aacgcattga
     6061 gctgggcgct catgtcgagc gcctgcgcgg cctgcgccgc gccggcatcg cgcgggcgga
     6121 tcggcgcgcc catcgtcacg cggtagcgcg cgccgcccag gcgctccacg cgcaccggca
     6181 ccagttcgca gtcctgcttc agcgcgagcc gcgcgggcac cgtgttggtc atcgcggcat
     6241 ggccgaagta cggcaccgcc tcgccctggt cgaggcgcgt gtcgcaggcg aggccgacgg
     6301 aatgcccggc gcgcagctcc gtgatcaggc tgcggatgcc gcccgtgctg ggtatccagc
     6361 gcacccggag ccgctcgcgc agctgcagca cctgcgcggc gatgagcggg ttgctctcgc
     6421 tcgcgtacag cgaggtgatg ggcagcccga agtgcgcgcc gaccaggttg gagagttgcc
     6481 aggcccccac gtgcgcggtg accagcacca tcggccgccc gccttcctgc aggccgcgga
     6541 tcgaggggtc ggcgcggaac tcgaggcgcc gctcgcgctc ggcccagatg cgctcgctct
     6601 gcaccagttc ggcgatcgcg gcgccgaggt gcgcgaagat gcgtcggcgc agccgcgcgc
     6661 gctcgtcagc cgggagcgcg ggaaaggcga tcgcgagatt gcgtgccacc ttgtcccaca
     6721 tcggcagcag cgggcccagc acgccggtca cgcgcatcgc gagcgcggtg gcgcgctcgg
     6781 gcgacagggc gcgcagcagc ccgatcacgc cacggatgaa ggcggcttcc agccaccacg
     6841 cggcgcgctt gaggccgggc agcgcacgcg cgagccgctt cgggatcagc aggtgctcgg
     6901 ccatgccgct tgcgccgctt gcgcgctcag gcgcgccgcg ccacgccgtc ggccgggaag
     6961 gtgccgaaga ccttgtccca cagcggcgag ctgacgccga agcgcttgcc ctcggcgccg
     7021 tagtggtgct gcaggtggta gaccttgagg taatgcagcc acgggtgctt catcgcgaaa
     7081 tggtgcgtgg cgtagtggat gtagtcgtag accaggtagc cgacgatgaa gaaggcacag
     7141 aagggctcga tccacggcgc cggcacgacc aggcggaaca cctgccacag caggaacatc
     7201 acgatgatcg cgccggccgg cggcatcacc aggcgcgtct tgtcgcgcgg ggcgtcgtgg
     7261 tgcacgccgt ggaacatgaa gaccaggtac ttgccgacgc ggccggtggc cgggtagtgg
     7321 aacacgaagc ggtgcagcag gtactcgctc agcgtccaca cgatgaggcc cgcgagcgcg
     7381 acgccgagca ggcccgcggc gggcagttcg tgcacgaaga cgctgcgcca cagcagccag
     7441 gccacgaccg ggctccagaa cagcagcggc acgatcgggt gcacgtgcgt cagtgcctcg
     7501 aggaagtcgt tgcgaaacag ccggatcgag ggttcctgct gcagggtgcg ggtgttgtca
     7561 gccatcggtg agctcccttg ctggcgacgg attggcggcg ggcggcgtcg gcgcggcatg
     7621 attctgcacg cgcccgcgtc gcacggcgta ggcggatttg ggccacaggc gcttggacgg
     7681 gaaccagtcg cccggacgcg cgcggatcca ctgctcgaac agcgcatgca cgctcccggt
     7741 catgcggatg gcgcgctcga tctcgtcccc gcccgcgggc accgcgacgg gcggatggaa
     7801 agtcacgcga aagcgcgccc cctgcaggcg ctcgacgcgg atcgggacca ggtcgaaacc
     7861 gtagcgcagc gcgaggcgcg cggggatcag cgtggtgcac ttggggtggc cgaacagcgc
     7921 gacgtccttg ccgctgtcga cgcgccggtc catcacgatg ccgaccgagg tgccctggcg
     7981 cagcgcgtgc agcatcgggc gcatgctctc gtcgcgcgcg agcagttcgc agccgatccg
     8041 cgtgcgccag cggttcagca actcgtcgag caggggattc gtgggcggcg catacactgc
     8101 cgcgagcggc acgttgcggc gcgcgatcgc ggcggcgatg acttcccagt tggcctggtg
     8161 cgcgctgacg aacaccgagg gctgcgtggc gtcgcgcagg gtgcggatgt cgccgaggac
     8221 ctcgatggca atgcgcgcac cgttctcgcc gaaggcgatt tcgtcgaggt gggcgtattc
     8281 ggcgaacacc gcgccggcgt tgccccaggc ctgacgcgcg atgcgcttgc gctcgtcctc
     8341 ggacttgtcg ggaaaggcga gacgcaggtt gtcatcgagc gcgcgcccct tgcgaaagcg
     8401 cggcccgagc gccgcgccga tgcgcgcgcc gaggcgcgag gcggcatcca ccggcaggcg
     8461 gcggatcagc cacagcagcg tgccgaaaaa ggcgcggtcg aggcggtaga gcacggcctc
     8521 gatggcgccg tggcgttcgc cgagcgcgcg cagtcgatgt ccgaggatga agcgtgccat
     8581 gcgaatcggt cagccctgac gagttacgcg cggagcagtg agcatggccg aagtctatcg
     8641 cagcgcgccg ggaggggcac ggcataactg acggcgcgaa agcggaactt tttaccgatt
     8701 ccgcgctgct cggcgtggtg ccggcaggct cgctatactg cgcgcaccgc ctgcatcggg
     8761 acacccgcat gatcgagatc gacatcggcg tcgcgcagcg cttcctggtg gccctgctga
     8821 tcggggcgct cgtcggcgtg gagcgcgaga agagccagag cgcgagcggt caccgttcga
     8881 tcgccgggct gcgcaccttc atcctgctgg cgctgctcgg ttcggtgtcg gcgtggattg
     8941 cgctgcacgt gggcggcgcg ctggtgttcg ccatcaccct gggcgcggtc gcgctggcgg
     9001 tggtgaccgg gtatgtgctg gagattcgcg cgcagcccga caagctggga ctgtcgagcg
     9061 aattcgcggc gatcgcggtg ttcctgctcg gcggcgtggt catgtacggc taccccgagc
     9121 tcgcggtggc gctcggcatc gtgacctcgg cggtgctcgc cttcaagcag ccgctgcacg
     9181 gcatggtggc gcgcgtgggc ctggacgata tctacgcggt gctgaagctg ctgatcgcga
     9241 gcttcatcgt gttgccgctg ctgcccaacc acgcggtgga tccctggggg gcgctgaatc
     9301 cctacacgct gtggctgctg gtgatcctga tctcgggcct gtcgctggtc gggtatgtcg
     9361 cggtcaggtt gctgggcgag gcgcacggta ccgtgctcac cggcttcgcc ggcgggctgg
     9421 tgtcatccac cgcggtgagc ctcagcttcg cgcgccagag ccgcaccgat ccaggcccgg
     9481 tggccgccga tgcgctcgcc gccggtatcc tgaccgcgtg ggcggtgatg ttcgtgcgcg
     9541 tgatgatcac cgtcgcgatc gtcaaccgcg agctgctgcc cacgctggtg cgcggattcg
     9601 gcgtgatggc gctggcggcg gcagggatgg cgctggcctt ctacgtgcgc gggctgcgcc
     9661 gtgccagcgt caccgcgcgc ggcgattcgc tgccggtgaa gaacccgttc agcctcggct
     9721 cggcggtgca gttcgcgctg ctgttcgcgg tggtgctggt gatcgtggag ctcacgcaaa
     9781 agtacgcgcc ggtcgaaggc ctctacgtcg tggccgccgt cgcggggctc accgacgtgg
     9841 atgcgatcac gctgtcgatg gcgcagggct cgcgcggtgg catcggcttc gcggtggcca
     9901 cgcacgcgat cggcatcgcc gcggtgtcga acacgctggt gaaatgcacc atggtgctgg
     9961 cgctggggcg cggcccgctg cgcccgcgac tcgcggtcgc gacggccgtg ctcgtcgcct
    10021 gcggggggct ggcggcgctc atcgtcaact gaacccccag accgcctgcc tgcgcgccga
    10081 cggcagctgg tcgatccatt gaacccgtga cctacgaggg acggggcttg cgtggccggg
    10141 tgctgcggtg cgggggcggc gtttcgccgc ggcgattgcc ggtgcccgcg ggctggaagc
    10201 tcggcaccag gtgcttcttg ccgttgccga tcagctcggc gcggcccatg cgtttgagcg
    10261 cctcgcgcag cagcggccag ttctcggcgt cgtggtagcg caggaaggcc ttgtgcaggc
    10321 ggcgcgtctt catcgcgcgc gccgtttcga cttcctcgcc gtcgcggcgc acgcgcttga
    10381 gcgggttgcg gcgcgagtgg tacatcgtgg tcgcgagcgc catcggggtc ggcaggaagg
    10441 tctgcacctg gtcgaggcga aagccgttgc gcttgagcca cagcgccagg ttcagcatgt
    10501 cctcgtcgcg cgtgccgggg tgtgccgcga tgaagtaggg gatcaggtac tgctccttgc
    10561 cggcctcgcg cgagaagcgg tcgaacatct ccttgaaacg ctcgtaggtg ccgatgcccg
    10621 gcttcatcat cttcgacagc gggccttcct cggtgtgctc gggcgcgatc ttcaggtagc
    10681 ccccgacgtg gtgctgcgcc agctccttga cgtactcggg gctttgcacg gccaggtcat
    10741 agcgcaggcc cgatccgatc aggatcttct tgatgccctt gagcccgcgc gcacggcggt
    10801 agatcttgac cagcgggcca tggtcggt
//
LOCUS       DAORUQ010000108        10797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6360_length_10797_cov_5.460343, whole genome shotgun sequence.
ACCESSION   DAORUQ010000108 DAORUQ010000000
VERSION     DAORUQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_10797_cov_5.460343"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PLW13_10285"
     CDS             <1..1855
                     /locus_tag="PLW13_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70808.1"
                     /translation="AASDEDLYDFREFTSALVPSERSNVYASAQLDLNERTAAIAEFG
                     FADNESEATLAPTPVFTAFESLPLTVSAANAYNPFGTDITDLRRRFVELGAREQLNRS
                     QTARTVLGLEGLAGSNWEWDATWHWSRTESEEILERLIDGDRLQHGIGPAADCRADAG
                     CVPVNLFGPPGSVDARQLDYLRAATQVSGQSRLYGLEANVSGPLASHAAGEIVLAAGA
                     SWRHESTASHTEPGNPAHALGGSDISAASGNRSVGEGWGEVLVPLWADGEDAHRVDLD
                     LAGRYSHYSDFGHTANGKVGLRLRPVPSVLLRGTWSQGFRAPSLLDLYETGQHTQAFL
                     TDPCAVAANVGTLPGCALPGDPTRTQYLAVIGGNAELEAEQSNSLTAGLVWTPAGAPG
                     LMVALDAFRIEQRDVVDASAQYILDLNARSGAFAERVLRDAEGNLQRVNATNINVGER
                     QVAGADLDLRYRLPALPVGRLTLSLETSYIHEYLDQLDPSAPSEDITGQFADEASGGM
                     GAIPQWKSRLGLQWSRAHWEASYRLYYASALKEQVPNSMRRREIDDWLVHDVQLVRQF
                     GLLEGLRVALGIDNVLDRAAPFSASAFNDNVDGRTHDLRGRYWYARLTQEF"
     gene            1858..>3523
                     /locus_tag="PLW13_10290"
     CDS             1858..>3523
                     /locus_tag="PLW13_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY70809.1"
                     /translation="MSARTRHLEAARRFQGLNQPAAAEAACRELLAREPGDREALELL
                     AWALVQRGELHGALDALQALSRAWPAEPRYLTKLAELALRTGASGLARAAYERLIAMH
                     PRVPLFRYLFAKVLTEANAPRAALAEYARALELGIDQPEETLVCMASLHSALHETAQA
                     EALLQRATGCKPDYVPALFNLATLVEERGERATALALYERVIALDPGFEQVFARVAQL
                     RRFERADDPLIARMLAELESPALSPGARESLHYGLGKAYDDCAEYVRAFRHYAAANAL
                     SRSRVPPYDPAAQERLIDAIITQCSREWFERVAPVADASPVFICGMFRSGTTLIEQVL
                     GSHPSITTGGELVYFPRRVAADIVPYPAALASIAPAVLQAIGSGYLDYLRESFPGAAR
                     VTDKRPDNFLYLGLIKALFPRARIVYASRNVLDNALSLWAQNLDARFNYANDLRHIVH
                     YNAQLQRLMAHWGTLFGEDILEVNYDRFVADQRPQTARLLEFCGLDWSDAALRFHERD
                     STVRTASLWQVRQPLYQRSSGRWRHYRDELGEILDYLRELGLAGMAE"
     assembly_gap    3524..3623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3728..4210
                     /locus_tag="PLW13_10295"
     CDS             3728..4210
                     /locus_tag="PLW13_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70810.1"
                     /translation="MHAPRRPLLHALLLATALLGANAGHAAGAPDVRELMSAEEFGAA
                     GLGRLTPAEIEALNAWLIRYTAGEAPVLRASSPAVREEVRKSEATVIRSRIAGPFAGW
                     SGRTLFRLENGQVWQQRMSGSWRHRADSPEVEIRKNLLGMWEMRVLESGRQVGVTRIE
                     "
     gene            4350..7124
                     /locus_tag="PLW13_10300"
     CDS             4350..7124
                     /locus_tag="PLW13_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70811.1"
                     /translation="MYKRSRLALSVAAAVGAIASTGSLPALAQDPAAQQIEEVVVTGS
                     RIRRTDLTSMSPISVFDEAALDRQGSTTLERFFVSLPSVSGADFGSGVNNGTAGWSTV
                     SLRGLGPTRTLVLLNGNRMASAGVNGFVDLNLIPSSLIERVEVLRDGASTIYGSDAIA
                     GVVNLITKSDFDGVEVEGQYDESGEGDGEQKLFAITFGKSFERGNIVVNGQYQERDEI
                     WQGERSFSECAFRERGSVAAGTRERFCGGSGTAYPATAYISANDFASPFVKDGGVPRP
                     YNAATDAFNYAAASYMVTPQDVYSFFSSGNYDLIESSPVGTVSAFMETQFSNRESAQL
                     LAPEGTFWGPLVPTTNPGNVFGEPAYAYRRLVETGGRRAEQDVNVWRGLIGLKGELNN
                     GWGWDLSYNRSEYRDDSVDLYRGNVPKMNTLIDPELCAADPACPGVWDPFAINTLTPE
                     MIAYGLSNARFTDESTLRTIQFNLTGDFGGLELPGGKPQWAVGYETREEEGSLTYDDN
                     VLAGNVYSYAGANTAADYEVDEFYAELNLPLIADMPFAHAVTAEISTRVSDYDFLGDE
                     EDVWKYALEWAPIADLRLRYTYSEGFRAPNIDEQASPEENTAATYTEPCVEWGANPDA
                     IVRANCAADGLRPDFTLSSDQAQSLQGGNANLAPEESESTTFGVVWTPSFLEDFSATV
                     DWYEIEIEGAIGSLGVDDIVTGCYSSANFSSPLCSLILGPAAVGENANPNSPRRNVIG
                     LISGPDIRLGNLSTFETKGIDFSMNYVFNAVWDGALNLSLDGTWLDSYEYTSGPGIPA
                     TELSGYYGTDPYQGDVPAAFPELKMNFVAAYSRDNWGGSYTIRWMDEVEDVASTCAGS
                     QLSCDADAYDYHDVQGYYEWKNVTFTAGVRNLWDEEPPYVSNFGDMNTLNFSYDTAGR
                     YYYTRISAKF"
     gene            7255..>8461
                     /locus_tag="PLW13_10305"
     CDS             7255..>8461
                     /locus_tag="PLW13_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY70812.1"
                     /translation="MPDPRRTLAEALELFRGKRYAQCARTLEPLCAAGEPAGALSLAA
                     MALSLAGEDEAATRCFERYLARQSADAAAWNNFGNHLRTREPGRAIDCYGKAIALKPD
                     FADARFNLALLHNERRAHRAALTALAPGAHAPCTPREHALAASLHLRLHEWNAAAPHL
                     EAQLAHEPADARTRLNLAVAQRMRGEPEAALRTLTAAGDAAPGALRFERASILLETGR
                     IGAARALFEHVIAQDPAHLGAHEALDKIHFETGAHERVGQSLAQALAQSADPALATAL
                     ALQYERIGARERAREVIERLAPAQRGTEAMRLHARLLVVAGHAEQAERELAACLAQHP
                     GERGARLDLARLLMRADRAPEAAQLLEHALAQHPGDQLLLANLGTAWKLCADARHEAL
                     FDVARLVRSY"
     assembly_gap    8462..8561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8562..9185
                     /locus_tag="PLW13_10310"
     CDS             <8562..9185
                     /locus_tag="PLW13_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HQY70813.1"
                     /translation="ADARHEALFDVARLVRSYTLLDFGLDADFLAELAALLRGMHHAT
                     HHPPDQSLRGGTQTVGNLLTRREAPIVRLRGAIEAVLAQYVAQLPTDAEHPFLRRRPE
                     RWRFAGSWSVLLRSQGFHVPHTHGQGWISSALYVALPQGLQGREGWLEFGRSDLQLPL
                     AQDPPALALAPQVGRLVLFPSYLWHGTVAFAAPGERLTVAFDVSPAN"
     gene            complement(9189..10526)
                     /locus_tag="PLW13_10315"
     CDS             complement(9189..10526)
                     /locus_tag="PLW13_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HQY70814.1"
                     /translation="MSDTGAAVAPLIGQARQCVARQEFARADALYGQLLEQGPVAARI
                     ESLRYFAMRAFRAGALDRAVDFSRRVTELEPGVSAHWLNLGVACQEAADLPAARAAFA
                     EAFRRDARNHTAALCIGEVLEATGDRTGAAMAYWRAIIDAQARGAWRNNETTPPALRP
                     RVVHAMDFVDVERPRLLQQVLAPLLEAYGEDEMRRIRAALRIYLGQEMPRYPDPRQKP
                     GFLYVPGLPTAAFLPLDVFPWIEELQARTAAIRDEMLAAVAAREGLQPFHDREQLNQL
                     VAGASAEGSWDALFFYRHGERFDANCARCPVTTAALERLPRVAIRDHGPEILFSLLRP
                     GAHILPHRGVTNARVVAHLPLVVPPGCALHVLGEEPHAWREGEVMVFDDTFEHEAWNR
                     SGELRVILLMDTWNPWLSEAERLACTRLVEGIGDFRLGGERIAERSRSDELFL"
     gene            complement(10523..>10797)
                     /locus_tag="PLW13_10320"
     CDS             complement(10523..>10797)
                     /locus_tag="PLW13_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY70815.1"
                     /translation="NDPLQSTILPVQWVGAMRQWIRTFREHPVTDFAPLIIQGSADET
                     VDWRWNLPMIRQKFPHAQLCMVDGARHHLANEVRHLREQVFAALAL"
BASE COUNT         1650 a         3808 c         3614 g         1525 t
ORIGIN      
        1 cgccgcctcc gacgaggacc tctacgactt tcgcgagttc acctcggccc tggtgccctc
       61 cgagcgcagc aatgtctacg cgagcgcgca gctggacctg aacgagcgca ccgcggcgat
      121 cgcggaattc ggcttcgcgg acaacgagtc cgaggccacg ctggccccca cgccggtatt
      181 cacggccttc gagagcctgc cgctgacggt ctccgcggcg aatgcgtaca accccttcgg
      241 cacggacatc acggacctgc gccggcgctt cgtcgaactc ggcgcgcgcg agcagctgaa
      301 tcgctcgcag accgcgcgca ccgtgctggg gctggaaggg ctggcgggca gcaactggga
      361 gtgggacgcg acgtggcact ggagccgcac cgagagcgag gagatcctcg agcgcctgat
      421 cgacggcgac cggctgcagc atggcatcgg ccccgccgcg gattgtcgcg ccgacgccgg
      481 ctgcgtgccg gtgaacctgt tcggcccgcc cggctcggtc gatgcgcggc agctggacta
      541 cctgcgcgcc gcaacgcagg tgagcggcca gtccaggctg tacgggctgg aggccaacgt
      601 ctccgggcca ctcgcgagcc acgcggccgg cgagatagtg ctcgccgccg gcgccagctg
      661 gcgccacgaa tccaccgcct cgcacaccga accgggcaac cccgcccatg cgctgggcgg
      721 cagcgatatc agcgcggcct cgggcaaccg ctcggtggga gagggctggg gcgaagtgct
      781 ggtaccgcta tgggccgacg gcgaggacgc tcaccgcgtg gacctggacc tggccgggcg
      841 ctactcgcac tacagcgatt tcggccacac cgccaacggc aaggtcggcc tgcgcctgcg
      901 accggtgccg agcgtcctgc tgcgcggcac ctggtcgcag ggattccgcg caccctcgct
      961 gctcgatctc tacgaaaccg gccagcacac gcaggctttc ctcaccgacc cctgcgcggt
     1021 ggccgcgaac gtcggcacgc tgcccggctg cgcgctgccc ggcgacccca cgcgcacgca
     1081 gtacctcgcg gtgatcggcg gcaacgccga gctcgaggcc gagcagtcga acagcctgac
     1141 cgccggcctg gtctggacgc ccgccggtgc gcccggactg atggtcgcgc tggatgcctt
     1201 tcgcatcgag cagcgcgacg tggtcgatgc cagcgcgcag tacatcctcg acctgaacgc
     1261 gcgcagcggc gccttcgccg aacgcgtgct gcgcgatgcc gagggcaacc tgcagcgcgt
     1321 caacgccacc aacatcaatg tcggcgagcg ccaggtcgcg ggggcggacc tcgacctgcg
     1381 ctaccgcctg cccgcgctgc ccgtcggccg gctgacgctg tcgctggaaa cctcgtacat
     1441 ccacgaatac ctcgaccagc tcgacccctc ggcgcctagc gaggacatca cgggccagtt
     1501 cgccgacgag gcatcgggcg gcatgggcgc cattccgcaa tggaagtcgc gcctcgggct
     1561 gcaatggagc cgcgcgcact gggaggcgag ctaccgcctg tactatgcga gcgcgctgaa
     1621 ggaacaggta cccaactcca tgcgccggcg cgagatcgac gactggctgg tgcacgatgt
     1681 tcagctggtg cgccagttcg ggctgctcga gggcctgcgc gtggccctcg gcatcgacaa
     1741 cgtgctggat cgcgcggcgc ccttctccgc ctcggccttc aatgacaacg tcgatggccg
     1801 cacccacgat ctgcgcggcc gttactggta cgccaggctg acccaggaat tctgagcatg
     1861 agcgcacgca cccggcacct cgaggcggca cggcgcttcc agggcctgaa ccagccggcg
     1921 gcagccgagg cagcgtgccg cgaactgctc gcgcgggaac ccggggaccg cgaagccctg
     1981 gagctgctcg cctgggcgct ggtgcagcgc ggcgagctcc acggcgcgct cgacgcgctg
     2041 caggcgctga gccgcgcctg gcccgccgag ccccgctatc tcacgaagct ggcggagctc
     2101 gcgctgcgca ccggcgcgag cgggctcgcg cgcgcggcct acgagcgact gatcgccatg
     2161 cacccgcgcg tgccgctgtt ccgctacctg ttcgccaagg tgctcacgga ggccaacgcg
     2221 ccgcgcgcgg cgctggccga gtacgcgcgc gcgctggagc tgggcatcga tcagcccgag
     2281 gaaacgctgg tgtgcatggc cagcctgcac agcgcgctgc acgagaccgc gcaggccgag
     2341 gccttgctgc agcgcgcgac cgggtgcaag cccgactatg tgccggcact cttcaacctg
     2401 gccacgctgg tcgaggaacg cggcgagcgc gccacggcac tcgcactcta cgagcgcgtc
     2461 atcgcgctcg acccgggttt cgagcaggtc ttcgcgcgcg tggcgcagct gcgcaggttc
     2521 gagcgcgccg acgatccgct gatcgcgcgc atgctggccg agctggagtc accggcgctc
     2581 tcgccggggg cgcgcgagag cctgcactac ggcctcggca aggcctacga cgactgcgcc
     2641 gagtacgtac gcgccttccg gcattacgcc gccgcgaatg cgctgagccg ctcacgcgtg
     2701 cctccctacg atcctgccgc gcaggagcgc ctcatcgacg cgatcatcac gcagtgctcg
     2761 cgcgagtggt tcgagcgcgt ggcgccggta gcggacgcga gcccggtgtt catctgcggg
     2821 atgttccgct ccggcaccac gctgatcgag caggtgctgg gctcgcaccc ctcgatcacc
     2881 acgggcggcg agctggtgta cttcccgcgc cgtgtcgccg ccgacatcgt gccctacccc
     2941 gcggcgctcg catcgatcgc gcccgcggtg ctccaggcga ttggcagcgg ctacctcgac
     3001 tacctgcgcg agtccttccc cggggccgcg cgcgtgaccg acaaacgccc cgacaacttc
     3061 ctctatctcg ggttgatcaa ggcgttgttt ccgcgcgcgc gcatcgtgta cgccagccgc
     3121 aacgtgctcg acaacgcgct gtcgctctgg gcccagaacc tcgatgcgcg cttcaactac
     3181 gccaacgacc tacgccacat cgtgcactac aacgcccagc tgcagcggct gatggcgcac
     3241 tggggcacgc tgttcggcga ggacatcctc gaggtgaact acgaccgctt cgtggccgac
     3301 cagcggccac agaccgcgcg cctgctcgag ttctgcggcc tggactggag cgacgcggcg
     3361 ctgcgctttc acgagcgcga cagcacggtg cgcaccgcga gcctgtggca ggtgcgccag
     3421 ccgctgtacc agcgctcctc gggccgctgg cggcattacc gcgacgagct cggcgaaatc
     3481 ctcgactacc tgcgcgagct gggcctggcc gggatggccg aggnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnctcggcg aaatcctcga ctacctgcgc gagctgggcc
     3661 tggccgggat ggccgaggac ccggcctgag cccgtatttc tccttcatcg aacaggaaca
     3721 gccaccaatg cacgctccgc gacgcccgct tctgcacgca ctgctgctcg ccaccgccct
     3781 gctcggcgcc aacgccggcc acgccgcggg ggcgcccgac gtgcgcgagc tcatgagcgc
     3841 ggaagaattc ggcgccgccg ggctgggccg gctgacgccc gcggagatcg aggcgctgaa
     3901 cgcctggctg atccgctaca ccgccgggga agcgccggtg ctgcgcgcga gcagccccgc
     3961 cgtgcgcgag gaggtgcgca agagcgaggc caccgttatc cgcagccgca tcgccggccc
     4021 gttcgcgggc tggagcgggc gtacgctgtt tcgcctcgag aacggccagg tctggcagca
     4081 gcgcatgagc gggagctggc gccaccgcgc ggactcgccc gaggtcgaga tccgaaagaa
     4141 cctgctcggc atgtgggaaa tgcgcgtgct cgaaagcggg cgccaggtcg gggtgacgcg
     4201 gatcgagtga tggcggcggc ggtaattttt cacatttctt tcgtggtcac cgattgagga
     4261 agtgtcatct tcgttcgtct caggtcttgt gaacggcttc cggccggcgg tcgggccgta
     4321 tccaattcca atcgacgagg agacaacgca tgtacaagag atcgcgtctg gcgctatccg
     4381 tagcagccgc cgtgggagcc atcgcatcga ccggcagcct gccggccctt gcccaggacc
     4441 cggctgccca gcagattgaa gaggtcgtcg tcaccggttc gcgcatccgg cgcaccgacc
     4501 tgaccagcat gagccccatt tccgtgttcg acgaggccgc gctggaccgc cagggctcca
     4561 cgacgctgga gcgcttcttc gtgagcctgc cctcggtgag cggcgccgac ttcggctccg
     4621 gcgtcaacaa cggcaccgcg ggctggtcca cggtgtcgct gcgcggcctc gggccgacgc
     4681 gcacgctggt gctgctgaac ggcaaccgca tggcctcggc cggcgtcaac ggcttcgtgg
     4741 acctgaacct gatcccgtcc tcgctgatcg agcgcgtcga ggtgctgcgc gacggcgcct
     4801 ccaccatcta cggttcggac gcgatcgccg gcgtggtcaa cctgatcacc aagtccgact
     4861 tcgacggcgt cgaggtcgag ggccagtacg acgagtcggg cgagggcgac ggcgagcaga
     4921 agctgttcgc gatcaccttc ggcaagagct tcgagcgcgg caatatcgtc gtcaacggcc
     4981 agtaccagga gcgcgacgag atctggcagg gcgagcgcag cttctccgag tgcgccttcc
     5041 gcgagcgcgg cagcgtcgcc gccggcacgc gcgagcgctt ctgcggcggc tcgggcactg
     5101 cctaccccgc gacggcctac atttcggcca acgatttcgc cagccccttc gtgaaggacg
     5161 gcggcgtacc gcgcccctac aacgccgcga ccgacgcgtt caactacgcg gccgcctcgt
     5221 acatggtgac gccgcaggac gtctacagct tcttcagctc gggcaattac gacctcatcg
     5281 agagcagccc ggtcggtacc gtcagcgcct tcatggaaac ccagttctcc aaccgcgaat
     5341 cggcccagct gctggcgccg gaaggcactt tctggggccc gctggtgccc accaccaacc
     5401 ccggcaacgt cttcggcgag ccggcctacg cctaccgccg cctggtggag accggcgggc
     5461 gccgcgccga gcaggacgtc aacgtctggc gcgggctgat cggcctgaag ggcgagctga
     5521 acaacggctg gggctgggac ctgtcgtaca accgctccga gtaccgcgac gactcggtgg
     5581 acctgtaccg cggcaacgtg cccaagatga acacgctgat cgaccccgag ctgtgcgcgg
     5641 ccgacccggc ctgccccggc gtctgggatc cgttcgcgat caacacgctg acgccggaaa
     5701 tgatcgccta cgggctgtcg aacgcgcgct tcaccgacga gtcgacgctg cgcacgatcc
     5761 agttcaacct gaccggtgac ttcggcggcc tcgagctgcc cggcggcaag ccgcagtggg
     5821 cggtcggtta cgagacgcgc gaggaagagg gcagcctgac ctacgacgac aacgtgctgg
     5881 ccggcaacgt ctacagctac gcgggcgcca acaccgcggc cgattacgag gtcgacgagt
     5941 tctacgccga actgaacctg ccgctgatcg ccgacatgcc cttcgcgcac gcggtgacgg
     6001 ccgagatctc gacgcgcgtg tccgactacg acttccttgg cgacgaggag gacgtgtgga
     6061 agtacgccct ggagtgggcg ccgatcgcgg acctgcgcct gcgctacacc tactccgagg
     6121 gcttccgcgc gccgaacatc gacgagcagg catcgccgga agagaacaca gcggccacct
     6181 acaccgagcc ctgcgtggaa tggggcgcca accccgacgc gatagtgcgc gccaactgcg
     6241 cggcagacgg cctgcgcccg gacttcacgc tgtcctcgga ccaggcacag agcctgcagg
     6301 gcggcaacgc gaacctggcg cccgaggaat cggaatccac cactttcggc gtcgtgtgga
     6361 ccccgagctt cctcgaggac ttctcggcca cggtggactg gtatgagatc gagatcgagg
     6421 gcgccatcgg ctcgctgggc gtcgatgaca tcgtgaccgg ctgctactcc agcgcgaact
     6481 tctcctcgcc gctgtgctcg ctgatcctcg ggccggctgc cgtgggcgag aatgcaaacc
     6541 cgaactcgcc gcggcgcaac gtgatcggcc tgatctcggg gccggacatc cgcctcggca
     6601 acctctcgac cttcgagacc aagggcatcg acttctcgat gaactacgtg ttcaacgcgg
     6661 tgtgggacgg ggcgctgaac ctctcgctgg acggcacctg gctcgacagc tacgagtaca
     6721 cctcggggcc gggcataccg gccaccgaac tctcgggcta ttacggcacg gatccctacc
     6781 agggtgacgt cccggccgcg ttccccgagc tgaagatgaa cttcgtggcc gcgtactcgc
     6841 gcgacaactg gggcggcagc tacaccatcc gctggatgga cgaagtggag gacgtggcca
     6901 gcacctgcgc cggcagccag ctgtcgtgtg acgccgacgc ctacgactac cacgacgtgc
     6961 agggctacta cgagtggaag aacgtcacgt tcaccgccgg cgtacgcaac ctgtgggacg
     7021 aggagccgcc ctacgtgtcg aacttcggcg acatgaacac gctgaacttc agctacgaca
     7081 ccgcgggccg ttactactac acccgcatca gcgcgaagtt ctgatccggc gtgaccgggg
     7141 gtcgccgcga ggcgaccccg catccttcaa gcccctctcc ggaggggctt ttttgttgtg
     7201 ggtcggcccg atcctgcaat aatccgcgcc tccaaccgtc acgacccgaa gccgatgccc
     7261 gacccgcgcc ggaccctcgc cgaagctctc gagctgttcc gtggcaagcg ttacgcgcaa
     7321 tgcgcgcgca cgctcgagcc gctgtgcgcg gccggcgaac ccgcgggcgc gctgtcgctg
     7381 gcggcgatgg cgctgtcact ggcgggcgag gacgaggcgg cgacgcgctg cttcgagcgc
     7441 tatctggcgc gccagtccgc ggatgccgcg gcgtggaaca acttcggcaa ccacctgaga
     7501 acgcgcgagc ccgggcgcgc gatcgactgt tacggcaagg cgatcgcgct gaagccggat
     7561 tttgcggatg cgcgcttcaa cctggcgctg ctgcacaacg agcgtcgcgc gcatcgcgcg
     7621 gcgctcacgg cgctcgcgcc cggtgcgcac gcaccctgta cgccgcgcga acacgcgctg
     7681 gcagcctcgc tgcacctgcg tctgcacgaa tggaacgcgg ccgcgccgca cctcgaggcg
     7741 caactcgcgc acgagcccgc ggatgcgcgc acgcgactga acctcgcggt ggcgcaacgc
     7801 atgcgtggcg agcccgaggc ggcgctgcgc acgctcaccg cggcaggcga tgcggccccc
     7861 ggggcgctgc gtttcgagcg ggcatcgatc ctgctcgaga ccggccgcat cggggcagcg
     7921 cgcgcgctgt tcgagcacgt catcgcgcag gaccctgcgc acctcggtgc gcacgaggcg
     7981 ctcgacaaga tccacttcga aaccggcgcg catgagcggg tagggcagag cctggcacag
     8041 gctctcgcgc agagcgccga tccggcgctc gccaccgcgc tggcgctgca atacgagcgg
     8101 atcggcgcgc gcgagcgtgc acgggaagtc atcgagcgcc tggcgcccgc ccagcgcggc
     8161 accgaggcca tgcgcctgca cgcgcgcctg ctggtggttg cgggccatgc ggagcaagcc
     8221 gagcgcgagc tcgccgcctg cctcgcgcag cacccgggtg aacgcggcgc gcggctggat
     8281 ctcgcgcgcc tcctgatgcg ggcagaccga gcgcccgagg ccgcgcagct gctggagcac
     8341 gccctggcgc aacatcccgg ggaccagctg ctgctggcaa acctcggcac agcctggaaa
     8401 ctctgcgccg atgcgcgcca cgaggcgctg ttcgatgtcg cgcgcctggt gcgcagctac
     8461 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgatgcg cgccacgagg
     8581 cgctgttcga tgtcgcgcgc ctggtgcgca gctacacact gctggacttc gggctggatg
     8641 cggacttcct cgccgagctc gccgccttgc tgcgcggcat gcaccacgcc acgcaccacc
     8701 cgccggacca gtcgctgcgc ggcggcacgc agacggtggg caacctgcta acgcgccgcg
     8761 aagcgcccat cgtgcgcctg cgcggtgcga tcgaggccgt gctggcgcag tacgtggcgc
     8821 agctgcccac cgatgcggag catcccttcc tgcgacggcg ccccgaacgc tggcgcttcg
     8881 cgggctcgtg gtcggtgctg ctgcgcagcc agggtttcca cgtgccgcac acccatggcc
     8941 agggctggat cagctcggcg ctctacgtcg cgctgccaca ggggctgcag ggcagggagg
     9001 gctggctgga gttcgggcgc agcgacctcc agctgccgct tgcgcaggac ccgcccgcgc
     9061 ttgcgctggc gccgcaggtg ggccggctgg tgctgttccc ctcgtatctg tggcacggca
     9121 cggtggcgtt cgccgcgccg ggcgagcggc tgaccgtcgc cttcgacgtg agccccgcga
     9181 actgagccct agaggaatag ctcgtcgctg cggctgcgtt cggcgatacg ctcgccgccg
     9241 aggcgaaagt cgccgatgcc ctcgacgagc cgcgtgcatg ccagtcgctc ggcctcgctg
     9301 agccagggat tccaggtgtc catcagcagg atcacgcgca gctcgccgct gcggttccac
     9361 gcctcgtgct cgaaggtatc gtcgaagacc atcacctcgc cctcgcgcca cgcgtgcggt
     9421 tcctcaccga gcacgtgcag cgcgcagccg ggcggcacca ccagcggcag gtgcgccacc
     9481 acgcgcgcgt tggtcacacc gcgatgcggc aggatgtggg cgccggggcg cagcagcgag
     9541 aacaggattt ccgggccgtg gtcgcggatc gcgacgcgcg gcagccgctc cagcgccgcc
     9601 gtggtaacgg gacagcgcgc gcagttcgcg tcgaagcgct cgccgtggcg gtagaagaac
     9661 agcgcgtccc agctgccctc cgccgatgct cccgcgacca gctggttcag ctgctcgcgg
     9721 tcgtggaagg gctgcaggcc ctcgcgcgcc gccaccgcgg ccagcatctc gtcgcggatt
     9781 gccgcggtgc gcgcctgcag ttcctcgatc catggaaaga catcgagcgg caggaaggcc
     9841 gcggtcggca ggccgggcac atagaggaaa ccgggtttct gccgcggatc cgggtagcgc
     9901 ggcatttcct gcccgaggta gatccgcagc gcggcgcgga tgcggcgcat ctcgtcctcg
     9961 ccataggctt ccagcaacgg cgccagcacc tgctgcagca ggcgcgggcg ctcgacatcg
    10021 acgaagtcca tcgcgtgcac cacgcgcggg cgcagtgccg gcggcgtggt ctcgttgttg
    10081 cgccaggcgc ccctggcctg cgcatcgatg atcgcgcgcc agtaagccat cgccgcaccg
    10141 gtgcgatcgc ccgtggcctc gagcacctcg ccgatgcaca gcgccgcggt gtggttgcgc
    10201 gcgtcgcggc gaaacgcctc ggcgaaggcc gcgcgcgcgg cgggcagatc cgcggcctcc
    10261 tggcaggcga cgccgagatt gagccaatgt gccgacacgc cgggctcgag ctccgttacc
    10321 cggcggctga agtccacggc gcgatcgaga gcgcccgcac ggaaggcgcg catcgcgaag
    10381 tagcgcaggc tctcgatgcg cgcggcgacc ggaccctgtt ccagcagctg gccgtagagc
    10441 gcgtcggcgc gcgcaaactc ctggcgcgcc acgcactggc gcgcctgccc gatcagtggc
    10501 gcaacggctg cgccggtgtc gctcacagcg cgagcgccgc gaatacctgc tcgcgcaggt
    10561 ggcgtacctc gttcgccagg tgatgacgcg cgccgtcgac catgcacagc tgcgcgtgcg
    10621 ggaacttctg ccggatcatc gggaggttcc accgccagtc caccgtctcg tcggcgctgc
    10681 cctgaatgat cagcggcgcg aaatccgtga ccggatgctc gcggaacgtg cggatccact
    10741 gccgcatcgc gccgacccac tgcaccggca ggatcgtgct ctgcagcgga tcgttgc
//
LOCUS       DAORUQ010000109        10701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6459_length_10701_cov_6.769021, whole genome shotgun sequence.
ACCESSION   DAORUQ010000109 DAORUQ010000000
VERSION     DAORUQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6459_length_10701_cov_6.769021"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLW13_10325"
     CDS             complement(<1..1040)
                     /locus_tag="PLW13_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQY70816.1"
                     /translation="MKRRTLLKQAVLAPLCLASSRLRAETAGAGWRSYALDYEIELPA
                     LKGPGQLWLPLPQDAGDYQRVGEITWRGDAPGATLRRDPVYGAPIFHAAWDGARGPRP
                     LTVSAVFATRDRPMAHKAPTPEASEAERFLQPTAHMPVDGIVAETAARIVGEAQAATP
                     DERARAIYDWIVDNTFREPTVRGCGLGDIKFMLESGNLGGKCADINSLFVGLARAAGI
                     PAREVYGLRVGPSAQFNCLGKPAGDVSGAQHCRAEFLSATHGWVPVDPADVRKAVLEE
                     KLPLEDARIAALRERLFGYWEMNWVAFNHARDFELAPKARAPLPYLMYPYAEFGDTVL
                     DGRDPAAFGFRIV"
     gene            complement(1216..1479)
                     /locus_tag="PLW13_10330"
     CDS             complement(1216..1479)
                     /locus_tag="PLW13_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQY70817.1"
                     /translation="MCRRALVSGTVQGVGYRAFARRAALELGIRGRAVNLPDGRVEVL
                     ACGEAAALDAFIAELNTGPKWSRVTQVEVSAAQCTAQPGFGSG"
     gene            complement(1482..1946)
                     /locus_tag="PLW13_10335"
     CDS             complement(1482..1946)
                     /locus_tag="PLW13_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQY70818.1"
                     /translation="MTPRIDRLRRAPGTLLLGVLLALAGCAREEEPTKAADTLRRPGQ
                     WLLVNYWAEWCKPCLEEIPELDAFAGKHAGTARVVLVNFDGVTGEALQQQARTLGIPP
                     ELLLDRDPATELGLARPQALPSTFVIDPSGAQRTVLLGPQTVASLEAATGSP"
     gene            complement(1930..3234)
                     /locus_tag="PLW13_10340"
     CDS             complement(1930..3234)
                     /locus_tag="PLW13_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HQY70819.1"
                     /translation="MTAHSRPPSGHADTGLRAMLADLGGFLHHLYARMTEDRTRQSAS
                     ALTFVSLFALVPMLTVSYAILSMVPDFEALDEQIQDFVFRHFVPSTGAEVQQYLRGFA
                     EQARRLTAAGTVMLVLSAYLMLKTIETQFNHIWRVREHRHGVANFLIYWAVLSLGPLL
                     LGAALLVSTYLFSLTVFSDAPQTLRVKSLALQALPQLFTFATFTLMYRVVPNCHVPLR
                     HAALGGALAALGFEAGKWLFTWTVGQGSYTLIYGTFAALPLFLLWIHLSWQILLGGAE
                     FVYALSTYRSRRAAQLPDLLVALGVLERLYRLHQTGSALRESELTGRDWLFGRYSTEP
                     VRWQRLSERLLDARLLRETQQGEYVLGIDADSVSLWSLYTLVGTAGSLPDASEREQLP
                     PWCRTALATIEAAERGLRQALAPSLEDIFTTGEQETGNDTAH"
     gene            3299..3652
                     /gene="arsC"
                     /locus_tag="PLW13_10345"
     CDS             3299..3652
                     /gene="arsC"
                     /locus_tag="PLW13_10345"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353290.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HQY70820.1"
                     /translation="MTEVSIYHNSRCSKSREALALLEERGIAPRVIAYMEAGLQEAEL
                     RALLAKLGIPARELLRTSEAAYRERGLGDPGLAEAALIGAMCAEPRLMQRPIVVRGKR
                     AVVARPAGRALELLA"
     gene            3649..>4172
                     /gene="wrbA"
                     /locus_tag="PLW13_10350"
     CDS             3649..>4172
                     /gene="wrbA"
                     /locus_tag="PLW13_10350"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQY70821.1"
                     /translation="MSDPWVLVLYYSRHGATARLADAVCEGVDSVRGIGARLRTVPAV
                     SAVCEAVAADVPDSGPLYCEEQDLRDCAGLVLGSPTRFGNMAAPLKYFIDGTSSLWLS
                     GALIDKPAAAFTSTASLHGGQESTLLSMMLPLLHHGMVFCGLPYSEAGLMQTRGGGTP
                     YGASHLAGAQGERAA"
     assembly_gap    4173..4272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4273..4418
                     /locus_tag="PLW13_10355"
     CDS             <4273..4418
                     /locus_tag="PLW13_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016501124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HQY70822.1"
                     /translation="GTPYGASHLAGAQGERAADGHELALAQALGRRIARLALALHAAR
                     GAH"
     gene            4418..4795
                     /locus_tag="PLW13_10360"
     CDS             4418..4795
                     /locus_tag="PLW13_10360"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HQY70823.1"
                     /translation="MDAVPAPRTALMRRISLACYTVLVAALSLQIFGIERLEVATRGF
                     LWVIWIGPLLVFLPGMVRGTWKTYLWLCFMLLVYFMVVVGNLFGPRAGPSDWLQLVLI
                     CVLFSTAMFYSRWRQRELAGQGA"
     gene            4811..6652
                     /gene="sppA"
                     /locus_tag="PLW13_10365"
     CDS             4811..6652
                     /gene="sppA"
                     /locus_tag="PLW13_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQY70824.1"
                     /translation="MDEKRKGPVRRLFGTLWSFLGWLRVSLANLVFLLVLLVLLALLT
                     LDRRPAVPDGGALVLDPSGAVVEQLSFVDPLAELLSGDENGQRETLLKDIVDAVRHAK
                     DDERIAMIVLATDAMEGAGVTKIGDIAKALEAFRATGRKVIAVGDAFSQDQYLLAAQA
                     DEIWMHPMGTVELSGYASYRNYYAGTLEKLKIDLRVFRSGTFKSAFEFLERGDMSEAD
                     RMATGDLLREIWSAYTTMVTAQRKLPPESVDHYANTLDAIYEKHGGDAGAAALEYGFV
                     DQLKSRIEMDQALKDMVGEDAEGNVNAMNFRDYLGAVRPVFALGETADKPAVGVIVAS
                     GMILDGERPSGSVGGDTLARLILQAADDDDIAALVLRIDSPGGSGFASEIIREALLAY
                     RDSGKPLVASMGSVAASGGYWIAAPADEIWASPTTITGSIGVLSAFPSFSRGLAELGV
                     GNDGVATTRTAGGLDPARPLSPAMAKVLELANHDAYRRFIGVVAEGRNMAPERVAEVA
                     EGRAWSGAQAAQNGLVDHLGDLDGAIAAAARLATLEDYRTQWIEEPRSWAGALLERLS
                     LSPQSLAATLLARGLGIDAAAEPLAPLLEGWRDPRAQYAICTVCAVY"
     gene            6637..7740
                     /locus_tag="PLW13_10370"
     CDS             6637..7740
                     /locus_tag="PLW13_10370"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQY70825.1"
                     /translation="MRGVLGLSLALVLALPAGCASVSYLGQAAYGQVQVLAAREPLQR
                     VIDDPHTEPELRARLELVRDLRAFAVSDLAMHEASGFEDYAALRRDFAVWNVVAAPEF
                     SLRPLTWCFPVAGCVAYRGYFGRDAAQRQGAALARQGLDTHVYGVAAYSTLGWFDDPV
                     LDTWVHRRESALAALIFHELAHQLVYAPDDTAFSESFARVVEREGVRRWFARSGSEAQ
                     LDAWLREEAVSAQFNALLASARRRLEALYVQPLAPEQMRARKAELFDGLRADYQRLSH
                     TWPEDMRFDAWMAAPLNNARLASVASYERWTPALTQLLQEHGGELESFYGAARTLAAL
                     APAAREARLLALERAANMAPPSVPDGAQQESPG"
     gene            7737..8078
                     /locus_tag="PLW13_10375"
     CDS             7737..8078
                     /locus_tag="PLW13_10375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQY70826.1"
                     /translation="MIRGRIASFGHAFEGLAVMIRTQPNAWIHSVATLAVVAAGAWMG
                     LAAHDWALLVVAIALVWVAEALNTAIEFLADAAVPDPHSLIKHAKDVAAGAVLLASAS
                     AVVIGALVFLP"
     gene            complement(8161..9261)
                     /locus_tag="PLW13_10380"
     CDS             complement(8161..9261)
                     /locus_tag="PLW13_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HQY70827.1"
                     /translation="MRAAVFEAAGQPLVIRDDVEIIAPRAGEVRVRVHYCGLCHSDYS
                     VVSGSFPFMGPVIVGHEASGVVESVGPGVTHLAPGDPVILTPLPPCGSCYFCQRGQHS
                     LCVNGIGLATMALPDGNTGLSRGGETLLRGVGLGALAEYVITPANGAIKVDPDVPLEV
                     ACVIGCAVQTGVGAVLNTAQVEAGATVLIQGLGGVGLATVQGARIAGAALIVAVDPVA
                     SRREAALAMGATHAFDPAAVNLGAEVMALTGNIGVDYAFETAGIAKLIESSLALTRSG
                     GMTVCVGAPPLEEKVTIENVVIFASMEKKLCGCLLGSCNSLREIPRMIALWRAGQLDL
                     EGLITARRPLAEVNEGFADMKAGRGIRTVIAI"
     gene            9471..10334
                     /locus_tag="PLW13_10385"
     CDS             9471..10334
                     /locus_tag="PLW13_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HQY70828.1"
                     /translation="MTVFTAVAAIGALTFVLAFLLNVANRRLRVFEDPRIDVVEEMLP
                     HANCGACGYPGCRAFAEAVVSGAALPGKCTVGTDEQRQRIAGFLGVAAGAEERRVARL
                     ACAGGANVARQRAHYHGPSGCRAAQAVGGGGKGCAWGCLGFGDCERSCNFNAIRMDGF
                     GLPRVDEAACTACGDCVSVCPRDLFSLQALGNRLWVACRNLEHGDTVLAECEVGCTAC
                     GRCAMDAADGLVSMQGNLPAVDYGCKQDRIPIERCPTGAIVWIDPALGPVRGAASAPV
                     VRQSRLRDGAS"
     gene            10380..>10701
                     /locus_tag="PLW13_10390"
     CDS             10380..>10701
                     /locus_tag="PLW13_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70829.1"
                     /translation="MNSKHSPCRFPGMPAAMDGNSAVIHCEREASDAAGAYPITPSTQ
                     MGEYWAEAVAQGHINVSGRPLIFIEPEGEHAAAAVTAGLAMTGLRAANFSSGQGIAYM
                     HESLY"
BASE COUNT         1485 a         3646 c         3910 g         1560 t
ORIGIN      
        1 acgatgcgaa agccgaaggc ggccggatcg cggccgtcga gcaccgtgtc gccgaactcg
       61 gcgtacgggt acatcaggta gggcagcggc gcgcgggcct ttggcgcgag ctcgaaatcg
      121 cgcgcgtggt tgaacgcgac ccagttcatt tcccagtagc cgaacagccg ctcgcgcagc
      181 gccgcgatgc gtgcgtcctc cagcggcagc ttttcctcca gcacggcctt gcgcacgtcg
      241 gccgggtcca ccggcaccca gccgtgggtc gcgctcagga actcggcgcg gcagtgctgc
      301 gcgcccgaga cgtcgcccgc gggcttgccg aggcagttga attgcgccga ggggccgacg
      361 cgcaggccgt agacctcgcg cgccgggatg ccggcggcgc gcgccaggcc cacgaacagc
      421 gaattgatgt cggcgcactt gccgccgagg ttgccgctct ccagcatgaa cttgatgtcg
      481 cccaggccgc agccgcgcac cgtcggctcg cggaaggtgt tgtcgacgat ccagtcgtag
      541 atcgcccgcg cgcgctcgtc gggcgtcgcg gcctgcgcct cgcccacgat gcgcgcggcc
      601 gtctcggcca cgatgccgtc gaccggcatg tgggcggtgg gctgcaggaa gcgttcggcc
      661 tcgctcgcct cgggcgtcgg ggccttgtgc gccatcgggc ggtcgcgcgt cgcgaacacg
      721 gcgctgacgg tcagcgggcg cgggccgcgc gcgccgtccc aggcagcgtg gaagatcggg
      781 gcgccataga ccggatcccg gcgcagtgtc gcgccaggcg cgtcgccacg ccacgtgatc
      841 tcgcccacgc gctggtagtc gccggcgtcc tggggcagcg gcagccacag ctggccgggc
      901 cccttcagcg ccgggagctc gatctcgtag tcgagcgcgt agctgcgcca gcccgcgccg
      961 gcggtttcgg cgcgcagcct ggacgaggca aggcagagcg gggcgaggac tgcttgtttc
     1021 aggagggtgc ggcgtttcat ggcgctcgat ccggatgaca tcggtgacgg ggagccaacc
     1081 ttacaccggt cgccggcacg gggcgagcgt ccgtcggctg gcgaatttcc cacgtcgcac
     1141 gtaggtcgcc attcatggcg acaacggcat ccggaacgca catgtcgcca taaatggcga
     1201 cctacggcgc tgtgctcagc cgctgccgaa gccgggctgc gccgtgcact gcgccgcgct
     1261 gacctccacc tgcgtgaccc gcgaccactt cggtcccgtg ttcagctcgg cgatgaaggc
     1321 gtcgagcgcc gcggcctcgc cgcaggccag cacctcgacg cgaccgtcgg gcaggttcac
     1381 ggcgcggccg cggatgccca gctccagcgc ggcgcggcgc gcgaaggcgc ggtaacccac
     1441 gccctgcacc gtgccgctga ccagcgcgcg ccggcacatg ctcagggact cccggtcgcg
     1501 gcctcgaggc tcgcgaccgt ctgcggcccc agcagcacgg tgcgctgcgc gcccgacggg
     1561 tcgatcacga aggtcgaggg cagcgcctgt ggccgcgcga ggccgagctc ggtggccgga
     1621 tcgcggtcga gcagcagctc gggcgggatg ccgagcgtgc gggcctgctg ctgcagcgcc
     1681 tcgccggtga cgccgtcgaa gttcaccagc accacgcgcg ccgtgccggc gtgcttgccg
     1741 gcgaaggcgt cgagctcggg tatctcctcc aggcagggct tgcaccactc ggcccagtag
     1801 ttcaccagca gccactgtcc cggccgacgc agcgtgtcgg cggccttggt gggctcctcc
     1861 tcgcgggcgc agccggccag cgccagcagc acgcccagca gcagcgtgcc cggcgcgcgc
     1921 cgtaatctgt caatgcgcgg tgtcattgcc tgtttcctgt tccccggtgg tgaagatgtc
     1981 ctcgaggctg ggcgccagcg cctggcgcag cccgcgctcg gcggcctcga tcgtggcgag
     2041 cgccgtgcgg caccacggcg gcagttgctc ccgttcgctg gcatcgggta gcgagcccgc
     2101 ggtgccgacc agcgtgtaca gcgaccacag cgacacgctg tcggcatcga tgccgagcac
     2161 gtattcgccc tgttgcgtct cgcgcagcag gcgcgcgtcg agcagccgct cgctcaggcg
     2221 ctgccagcgc acgggctccg tgctgtagcg cccgaacagc cagtcgcggc ccgtgagttc
     2281 gctctcgcgc agcgcgctgc cggtctggtg caggcgatag aggcgctcca gcacgccgag
     2341 cgcgaccagc aggtcgggca gttgcgcggc gcgccggctg cggtaggtcg agagcgcgta
     2401 gacgaactcg gccccgccga gcaggatctg ccatgacagg tggatccaca gcaggaacag
     2461 cggcagcgcg gcgaaggtgc cgtagatcag cgtgtagctg ccctggccca cggtccaggt
     2521 gaacagccat ttgccggcct cgaacccgag tgcggccagc gcgccgccga gcgcggcatg
     2581 gcgcagcggc acgtggcagt tgggcaccac gcggtacatc agcgtgaagg tggcgaaggt
     2641 gaacagctgc ggcagcgcct gcagcgccag ggacttcacg cgcagggtct gcggcgcgtc
     2701 gctgaaaacc gtcagcgaga acaggtaggt gctgaccagc agcgcggcgc cgagcagcag
     2761 cggccccagg ctcagcacgg cccagtagat caggaaattc gccaccccgt ggcgatgctc
     2821 gcgcacgcgc cagatgtgat tgaactgggt ctcgatggtc ttcagcatca ggtaggccga
     2881 gagcaccagc atcaccgtgc cggcggccgt gagccgccgc gcctgctcgg cgaagccgcg
     2941 caggtactgc tgcacctcgg cgccggtgga gggcacgaaa tggcggaaca cgaagtcctg
     3001 gatctgctcg tccagcgcct cgaagtccgg caccatcgag aggatcgcgt aggacacggt
     3061 gagcatcggc accagcgcga acagcgatac gaaggtgagt gccgaggcgc tctggcgcgt
     3121 gcggtcctcc gtcatgcgcg cgtagaggtg gtgcaggaag ccgccgaggt cggccagcat
     3181 ggcgcgcagg cccgtgtcgg cgtggccgga gggtggccgg gaatgtgctg tcatgcgcgt
     3241 gcggttatac tgcgcggcct cgaagtgcgg cattagaaca gaagctcgcg gatacgtcat
     3301 gaccgaagtc tcgatctacc acaactcccg ctgctccaag tcgcgcgagg cgctggcgct
     3361 gctggaggag cgcggcatcg ccccgcgcgt gatcgcctac atggaggccg gcctgcagga
     3421 ggcggaactg cgcgcgctgc tggcgaagct cggcatcccg gcgcgcgagc tgctgcgcac
     3481 ctcggaggca gcgtaccgcg agcgcgggct gggcgatccg ggcctcgccg aggccgcgct
     3541 gatcggggcg atgtgcgccg agccgcgcct gatgcagcgc ccgatcgtgg tccggggcaa
     3601 gcgcgccgtg gtggcgcgcc cggccgggcg cgcactggag ctgctcgcat gagcgatccc
     3661 tgggtgctgg tgctgtacta cagccgccac ggcgccacgg cgcggctcgc cgacgcggtg
     3721 tgcgagggcg tggattcggt gcgcggcatc ggcgcgcggc tgcgcacggt gccggcggtg
     3781 tcggcggtct gcgaggcggt ggccgcggac gtgcccgaca gcgggccgct ctactgcgag
     3841 gagcaggacc tgcgcgactg cgccggcctg gtgctgggca gcccgacgcg tttcggcaac
     3901 atggccgcgc cgctcaagta tttcatcgac ggcacctcga gcctgtggct gagcggggcg
     3961 ctgatcgaca agccggccgc ggccttcacc tcgaccgcga gcctgcacgg cgggcaggag
     4021 agcacgctgc tcagcatgat gctgccgctg ctgcatcacg gcatggtgtt ctgcgggctt
     4081 ccctacagcg aggccggact gatgcagacc cgcggcggcg gcacgcccta cggcgcctcg
     4141 cacctggccg gcgcgcaggg cgagcgcgcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nngcggcacg ccctacggcg cctcgcacct ggccggcgcg cagggcgagc
     4321 gcgccgccga cgggcacgag ctcgcgctgg cgcaggcgct gggacggcgc atcgcgcgcc
     4381 tcgcgctggc gctgcacgcg gcgcgggggg cgcattgatg gacgccgtcc ccgccccccg
     4441 caccgcgctg atgcgccgca tctcgctcgc ctgctacacc gtgctcgtgg cggcgctgtc
     4501 gctgcagatc ttcggcatcg agcggctcga ggtcgccacg cgcggtttcc tgtgggtgat
     4561 ctggatcggg ccgctgctgg tattcctgcc ggggatggtg cgcggcacct ggaagaccta
     4621 cctgtggctg tgtttcatgc tgctggtcta cttcatggtc gtggtgggca atctgttcgg
     4681 gcccagggca gggccttccg actggctgca gctcgtgctg atctgcgtgt tgttcagcac
     4741 cgcgatgttc tactcccgct ggcggcagcg ggaacttgcc ggccagggtg cctgaggaga
     4801 aatgcaagca atggacgaga agcgcaaggg gccggttcgc cgcctgttcg ggacgctgtg
     4861 gtcgttcctg ggctggttgc gcgtctcgct cgcgaacctg gtgttcctgc tggtgctgct
     4921 ggtgctgctg gcgctgctga ccctggaccg gcggcccgcg gtgcccgatg gcggcgcgct
     4981 ggtgctcgat ccctcgggcg cggtggtcga gcagctgagc ttcgtcgatc cgctggcgga
     5041 actgctgagc ggcgacgaga atggccagcg cgagacgctg ctgaaggaca tcgtggatgc
     5101 cgtgcgccac gcgaaggacg acgagcgcat cgcgatgatc gtgctcgcca ccgatgcgat
     5161 ggaaggcgcc ggcgtgacca agatcggcga tatcgccaag gcgctggaag ccttccgcgc
     5221 cacggggcgc aaggtgatcg cggtgggcga tgctttcagc caggaccagt acctgctcgc
     5281 ggcgcaggcc gacgagatct ggatgcaccc gatgggcacg gtcgagctgt ccggctacgc
     5341 ctcctaccgc aattactacg ccggcacgct ggagaaactg aagatcgacc tgcgcgtgtt
     5401 ccgctccggg accttcaagt ccgccttcga gttcctcgag cgcggcgaca tgtcggaggc
     5461 cgaccgcatg gccacgggcg acctgctgcg cgagatctgg agcgcctaca ccacgatggt
     5521 caccgcgcag cgcaagctgc cgcccgaatc ggtggaccac tacgccaaca cgctcgatgc
     5581 catctacgag aagcacggcg gcgacgccgg cgcggcggcg ctggaatacg gtttcgtcga
     5641 ccagctgaag agtcgcatcg aaatggacca ggcgctcaag gacatggtcg gcgaggacgc
     5701 cgagggcaac gtgaacgcga tgaacttccg cgactacctc ggcgccgtgc gcccggtctt
     5761 cgcgctcggc gagacggcgg acaagccggc tgtcggcgtg atcgtcgcca gcggcatgat
     5821 cctcgacggc gagcggccct cgggcagcgt cggcggcgat acgctggcgc ggctgatcct
     5881 tcaggcggcc gatgacgatg acatcgccgc gctggtgctg cgcatcgact cgcccggcgg
     5941 cagcggcttc gcctcggaga tcatccgcga ggcgttgctc gcctatcgcg acagcggcaa
     6001 gccgctggtg gcctcgatgg gcagcgtcgc ggcctcgggc ggttactgga tcgccgcgcc
     6061 cgccgacgag atctgggcca gcccgaccac gatcaccggc tcgatcgggg tgctcagcgc
     6121 ctttccgagc ttctcgcgcg gtcttgccga gctcggcgtc ggcaacgacg gcgtggcgac
     6181 cacccggacc gccggcgggc tcgacccggc gcgtccgctg tcaccggcga tggcgaaggt
     6241 gctggagctc gcgaaccacg acgcctaccg gcgcttcatc ggcgtcgtgg ccgagggacg
     6301 caacatggcg cccgagcgcg tcgccgaggt ggccgagggg cgcgcgtgga gcggcgcgca
     6361 ggcggcgcag aacgggctcg ttgaccacct cggcgacctc gatggcgcga tcgcggccgc
     6421 cgcgcgcctg gcgacgctcg aggattaccg cacgcagtgg atcgaggagc cgcgctcctg
     6481 ggccggcgcg ctgctcgagc gcctgtcgct gtcgccgcaa tcgctggccg cgacgctgct
     6541 cgcgcgcggg ctcggcatcg atgccgccgc cgagccgctc gcgccgctgc tcgagggctg
     6601 gcgcgacccg cgcgcgcagt acgcgatctg cacggtgtgc gcggtgtact aggtctttcg
     6661 ctggcgctcg tgctggcgct gccggccggc tgcgccagcg tgtcctacct ggggcaggcc
     6721 gcctacggcc aggtgcaggt gctggcggcg cgcgagccgc tgcagcgcgt gatcgacgat
     6781 ccgcacaccg agccggagct gcgcgcccgt ctcgagctgg tgcgcgatct gcgcgccttt
     6841 gccgtttccg atctagccat gcacgaggcc tcgggattcg aggactatgc ggcgctgcgg
     6901 cgcgacttcg ccgtatggaa cgtcgtggcc gcgcccgagt tctcgctgcg cccgctcacc
     6961 tggtgctttc ccgtggccgg ctgcgtggcg tatcgcggct acttcggccg cgacgccgcg
     7021 cagcgccagg gcgccgcgct ggcgcggcag gggctcgata cgcatgtcta cggtgtggcc
     7081 gcctactcca cgctgggctg gttcgatgat ccggtgctcg acacctgggt gcatcggcgc
     7141 gagagcgcgc tggccgcgct gatcttccac gagctcgcgc accagctcgt ctacgccccc
     7201 gacgacaccg ccttctccga gagcttcgcg cgcgtggtcg agcgcgaggg cgtgcggcgc
     7261 tggtttgcgc gcagcgggag cgaggcgcag ctcgacgcct ggctgcgcga agaggcggtc
     7321 agcgcgcaat tcaacgcgtt gctcgccagc gcgcgcaggc ggctggaggc cctttacgtg
     7381 cagccgctcg cgcccgagca gatgcgcgcg cgcaaggcgg agctgttcga cgggttgcgc
     7441 gccgactacc aacgcctcag ccacacctgg cccgaggaca tgcgcttcga tgcgtggatg
     7501 gcggcgccgc tcaacaacgc gcgcctggcg agcgttgcca gttacgagcg ctggacgccg
     7561 gcgctgacgc agttgctgca ggagcacggc ggcgagctgg agtccttcta cggcgcggcg
     7621 cgcacgctgg ccgcgctcgc gccggcggcg cgggaggcgc gattgctggc gctggagcgc
     7681 gcggctaaca tggcgccgcc gtccgtgccg gacggcgcgc agcaggagag cccaggatga
     7741 tccgcggtcg catcgcgagc tttggccatg ccttcgaggg gctggccgtg atgatccgca
     7801 cccaacccaa cgcctggatc cacagcgtgg cgacgctcgc ggtggtggcg gcaggggctt
     7861 ggatggggct ggcggcgcac gactgggcgc tgctggtcgt cgcgatcgcg ctggtgtggg
     7921 tggccgaggc gctgaacacg gcgatcgagt tcctggccga tgcggcggtg cccgatccgc
     7981 actcgctgat caagcacgcc aaggacgtgg cggcgggcgc ggtgctgctg gcctcggctt
     8041 cggctgtggt gatcggcgcg ctggtgttcc tgccgtagct gcgcctgtgg gtcgccattc
     8101 atggcgacag cagcattgcc gaaaggcaca tgtcggcatg aatgccgacc cacggagcgc
     8161 tcagatcgcg atcacggtgc ggatgccgcg gccggccttc atgtccgcga agccctcgtt
     8221 gacctcggcg agcgggcggc gcgcggtgat gagtccttcc aggtcgagct gccccgcgcg
     8281 ccacaacgcg atcatgcgcg ggatctcgcg cagcgagttg cagctgccga gcaggcagcc
     8341 gcagagtttc ttctccatcg aggcgaagat caccacgttc tcgatcgtga ccttttcctc
     8401 gagcggcggc gcgccgacgc acacggtcat gccacccgag cgcgtcagcg ccagcgaact
     8461 ctcgatcagt ttcgcgatgc ccgcggtctc gaaggcgtag tcgacgccga tattgccggt
     8521 gagcgccatc acctcggcgc cgagattcac cgcggccggg tcgaacgcat gcgtcgcgcc
     8581 catcgcgagc gcggcctcgc ggcgcgaggc gaccgggtcc accgcgacga tgagcgccgc
     8641 accggcgatg cgcgcgccct gcaccgttgc gagccccaca ccgccgagcc cctggatcag
     8701 caccgttgcg ccggcctcga cctgcgcggt gttcagcacc gcgcccacgc cggtctgcac
     8761 cgcgcagccg atcacgcagg ccacctcgag cggcacgtcg gggtcgacct tgatcgcgcc
     8821 gttcgccggc gtgatcacgt actcggccag cgcgccgagg ccgacgccgc gcagcagcgt
     8881 ctcgccgccg cgcgagagcc cggtgttgcc gtcgggcagc gccatcgtgg cgagtccgat
     8941 gccgttcacg cacaggctgt gctggccgcg ctggcagaaa tagcagctgc cgcagggcgg
     9001 cagcggcgtg agtatcaccg ggtcgccggg cgcgagatgg gtgacgcctg ggcccaccga
     9061 ctcgaccacg ccggaggcct cgtgacccac gatcaccggg cccatgaacg ggaagctgcc
     9121 ggagaccacg ctgtagtcgg agtggcacag gccgcagtag tgcacgcgca cgcgcacctc
     9181 gccggcgcgc ggcgcgatga tctcgacgtc gtcacggatg accagcggct ggcccgcggc
     9241 ttcgaacacg gctgcacgca tcactcttgc tccggaatcg ctcgggggcc cgcagcctag
     9301 ccagccggtc gcgccacggt caagacgcag gcggcagatc catccgtggt tgatgtggat
     9361 caagcggatc cgtggccggt tgcatgcggg cgcgtggcgt tttgacagcc cccgccacgg
     9421 cgcatagagt cgtgcctcga ttcctgcgaa tggcacgggc gtgggtgcgc atgactgtat
     9481 tcacggcggt ggccgccatc ggcgctctga ctttcgtgct ggcgttcctg ctgaatgttg
     9541 ccaatcgcag gttgcgcgtg ttcgaggatc cgcgcatcga tgtggtcgag gagatgctcc
     9601 cgcacgccaa ctgcggtgcc tgcgggtatc cgggttgccg ggccttcgcc gaggccgtgg
     9661 tgagcggcgc ggcactgccg ggcaagtgca ccgttggcac cgacgagcag cgccagcgca
     9721 tagccggctt cctcggcgtg gccgcgggcg ccgaggagcg gcgcgtggcg cggctggcct
     9781 gcgccggcgg ggcgaacgtg gcgcgccagc gcgcgcatta ccacgggccg tcgggttgcc
     9841 gcgcggcaca ggccgtcggt ggcggcggca agggctgcgc ctggggttgc ctcggcttcg
     9901 gcgactgcga gcgcagctgc aatttcaacg cgatacggat ggacgggttc gggctgccgc
     9961 gggtggacga agcggcctgc acggcctgcg gggattgcgt cagcgtctgc ccgcgcgacc
    10021 tgttctcgct gcaggcgctc gggaaccggc tgtgggtggc gtgtcgcaac ctcgagcacg
    10081 gcgatacggt gctcgcggag tgcgaggtcg ggtgcaccgc ctgcgggcgc tgcgcgatgg
    10141 atgccgccga tggcctggtg agcatgcagg gcaacctgcc ggcggtcgat tacggttgca
    10201 aacaggacag gataccgatc gagcgctgcc cgaccggggc catcgtctgg atcgatcccg
    10261 cgctcgggcc ggttcggggc gcggcgagcg cgccggtcgt gcggcaatcg cgcctgcgcg
    10321 acggcgcgag ctgaccgggc cagcgctccg cacgggcaca gcatgagcat ggagaacgga
    10381 tgaacagcaa gcacagcccc tgcaggttcc cgggcatgcc cgcggcgatg gacggcaaca
    10441 gcgccgtgat ccactgcgag cgcgaggcca gcgacgccgc cggcgcctac ccgatcacgc
    10501 cctcgacaca gatgggcgag tactgggccg aggcggtggc ccagggccac atcaacgtct
    10561 cgggccgccc gctgatcttc atcgagcccg agggcgagca cgcggcggcg gcggtgaccg
    10621 cgggcctggc gatgaccggg ctgcgggccg cgaacttctc ctccgggcag ggcatcgcct
    10681 acatgcacga gtcgctctac g
//
LOCUS       DAORUQ010000110        10517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6655_length_10517_cov_5.612694, whole genome shotgun sequence.
ACCESSION   DAORUQ010000110 DAORUQ010000000
VERSION     DAORUQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_10517_cov_5.612694"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLW13_10395"
     CDS             <1..779
                     /locus_tag="PLW13_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQY70830.1"
                     /translation="FGDAAYEVIPVYGGRVFRPAQHFERLRASLAVLGIAPPHDAAGW
                     SAIIARLLADAGPREHSLYIHVSRGVDAGRDAAHFTGLTPTVLAMVEPVTLDRAVLRQ
                     GIRAALLEDTRWHNCHVKSTSLLGNLLLRRRALDDGAAEALLHRNGELTEGTSSNVFA
                     VLGGELRTAPADRRILRGITRDVVIELAGACAIPVAERAPALTELAQAAELFICSSTR
                     EIVPVTVLDGRPVGDGAPGPQTRRLIAAFDDFKRAFMEAA"
     gene            776..1045
                     /locus_tag="PLW13_10400"
     CDS             776..1045
                     /locus_tag="PLW13_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQY70831.1"
                     /translation="MSDIEAPRIEFPCDYPVKIIGDAAADFRAVVEDIVERHAGGYDR
                     ALTTERSSANGRFVSLTVTIVATGPAQLQSLFADLKATGRVHMVL"
     gene            1069..1731
                     /gene="lipB"
                     /locus_tag="PLW13_10405"
     CDS             1069..1731
                     /gene="lipB"
                     /locus_tag="PLW13_10405"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQY70832.1"
                     /translation="MLEVARLDRVDYALAWRAMQAYTDTRTPQSPDRIWLLQHPCVFT
                     LGQAGKREHLRDTGATPVIHVDRGGQVTWHGPGQCVAYLMLDLRRMGLGVRELVERIE
                     AAIIATLGHFGIEGVARRDAPGVYVDGAKIAALGLRVRRGCCFHGLALNIDPDLGAFA
                     RIDPCGHPGMAVTRIADLVPVGTDVSRARVEALLLGELLAVFGYDPARVRECGATELP
                     AP"
     gene            1865..3907
                     /locus_tag="PLW13_10410"
     CDS             1865..3907
                     /locus_tag="PLW13_10410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQY70833.1"
                     /translation="MKRSLSLATLCVAALFGGFAHGAPSASPYANASGIDRGGFDDKV
                     RPQDDFFDYVNGGWIAATAIPPDRAWWGVVPELRAESEARQRAIIEEMAARSDLGPDS
                     VARKIGAFFASLTDQSLVEGKGVAPIEGTLARIAAIDSTESLAAAFGAAQLLGIDAPL
                     AVGVVQDPGAADRYVPYIWQSGIALPDRDYYLRDDEKFRKIRAAYPGYIAKLLGLGGF
                     GGTPEQGQAVFAIEQRLAELHWPAQENRDMKKLYNMVPAAQLTAVAPDFAWSQYIDAA
                     GLGARPELMAAQLSYVKQIGAIVGEFPLEAWKDYLRYHALNSAAPWLSGEFEQANFEF
                     MQREVLGLTELAPEWKRAVRAIDALAGEAVGQVYVERYFPAGHKQAMLGLVDDLMEAF
                     RTGITELEWMSPETRAKAEAKRARLATKIGYPDKWRDYSGLEVRADDPLGNLARASAF
                     EYRHELAKLDQPIDRAEWSMTPQTVNAYHQPFMNEIVFPAGFLQPPNFNMAADPAVNY
                     GAIGYVIGHEIGHAFDDKGRAFDPYGKLNDWWSAADAEHFEQAAAKLVEQYNAFRPLP
                     DMSINGKLTLGENIADLTGITIAYRAYRASLGGKEAPVIDGLSGDQRFFIGFAQANRA
                     SMREEMLRSMLVSDPHSPARYRVLGVLRNFTPFYAAFGVKEGDGMYLPPDERVKIW"
     gene            4016..4999
                     /gene="lipA"
                     /locus_tag="PLW13_10415"
     CDS             4016..4999
                     /gene="lipA"
                     /locus_tag="PLW13_10415"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQY70834.1"
                     /translation="MTEQSTSSRPAPRKAVQGEKLRGAEKVARIPVKVIPTVELPRKP
                     DWIRVRVPASPEVTRIKEILRRHKLASVCEEAACPNLGECFSGGTATFMIMGEICTRR
                     CPFCDVAHGRPNPLDAGEPRGLAEAIAEMKLRYVVVTSVDRDDLHDGGAAHFAACIGE
                     TRALNPGIRVEVLVPDFRGRMDVALDILEREPPDVFNHNLETVPRLYLQSRPGSDYAW
                     SLDLLRQYKQRCPEVLTKSGLMLGLGETNEEVVEVMRDLRAHDVDMLTLGQYLQPSRN
                     HLPVDRFVPPAEFEELGRIAQSLGFRSVASAPLVRSSYHADKQVDLSLLKS"
     gene            5131..>5455
                     /locus_tag="PLW13_10420"
     CDS             5131..>5455
                     /locus_tag="PLW13_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQY70835.1"
                     /translation="MYELYIANKNYSSWSLRPWVLMRELRIAFDERLVPFAPGSNFER
                     FRAFSPTGKVPCLHDGATVVWDSLAIAEYLAERHAGVWPSDARARAWARSAAAEMHSG
                     FGALRQ"
     assembly_gap    5456..5555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5556..5972
                     /locus_tag="PLW13_10425"
     CDS             <5556..5972
                     /locus_tag="PLW13_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQY70836.1"
                     /translation="WARSAAAEMHSGFGALRQRASMSCGQRVRLKELPADLRADLARL
                     EALWTEGLARFGGPFLAGAAFGAVDAFYAPVAFRLQTYALPLAEPAAAYAMRLLALAA
                     MRDWYAAALAEPWRDEPHEADIAAAGTITQDLRRAP"
     gene            complement(6030..7037)
                     /gene="holA"
                     /locus_tag="PLW13_10430"
     CDS             complement(6030..7037)
                     /gene="holA"
                     /locus_tag="PLW13_10430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQY70837.1"
                     /translation="MRIRVEDFPARLKARLFPFYLLFGDEPLLVQECADALRAACRNG
                     GIGERQAFDVDAGFDWSRLSAECSGLSLFGDRKLIEVRLAGAKLGAEGSAMLRELAAQ
                     PASDNVVLVLGGKLDRDVEKSAWFRALDEAGATVRAWPLRRNELPGWIGQRLRAAGFK
                     PSPDAVALLAERVEGNLLAARQEIDKLALLAEPGPLDAATLAALVNDSARYSVYDLCD
                     RALEGDRAAAVRTLTGLRAEGTEAAIVLWALAEEFRRLLRIGRRRATGEGSEQATKAE
                     RGKPYSEAAFRRLGHQGLQQLLRLAAEVDRCIKGLDKRDAWDALLELVVRAGRPANPR
                     P"
     gene            complement(7046..7558)
                     /gene="lptE"
                     /locus_tag="PLW13_10435"
     CDS             complement(7046..7558)
                     /gene="lptE"
                     /locus_tag="PLW13_10435"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQY70838.1"
                     /translation="MRNTLLRSVSLALITVLTLAACGWHPRGSQEVPLQMRQLRLVTE
                     QPNAALTLKLRRALQGAGVELVDVAGAGPSLHIGTEQRVSRKISLDRDARSAEQEMRV
                     SVAFDLRNADGEVVFGPRTASANRIYAFDPNSIIAKQDEENLIYQELLDNVVGQVLRQ
                     LRRAQAGLAP"
     gene            complement(7565..10033)
                     /gene="leuS"
                     /locus_tag="PLW13_10440"
     CDS             complement(7565..10033)
                     /gene="leuS"
                     /locus_tag="PLW13_10440"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041656989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQY70839.1"
                     /translation="MEELYDAERTEADAQAFWEHNRSFVVTEDPAREKFYCLSMFPYP
                     SGRLHMGHVRNYTLGDVIARFHRMQGRNVLQPMGWDAFGLPAENAAIQNNTAPARWTH
                     ANIDYMRGQLKRLGFAYDWSRELATCQPAYYRWEQWFFTRLYEKGLAYKKVSAVNWCP
                     KDATVLANEQVIEGCCWRCDTPVERREIPQWFIRITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGIEMSFPLATPLAGITEVPIYTTRPDTLLGVTYLAIAGEHPLAKALAE
                     GRPELADFIAECRRSSVAEADMATMAKKGLDTGLKALHPLSGREVPIWVANFVLMEYG
                     TGAVMSVPGHDQRDWEFARAYGLPIEQVIAPLDPATPCDLASAAFTEKGRLLNSGEFD
                     GLGFEQAFDAIAARLAANGNGRVTLNYRLRDWGVSRQRYWGAPIPMLYLADGGEIPVP
                     ADRLPVLLPEDVEMSGVQSPIKANPAWRRSTLAGQEVEHETDTFDTFMESSWYYARFT
                     CPDLETAMLDPARANYWLPVDQYIGGIEHAILHLLYSRFFHKLMRDEGLVTSDEPFTR
                     LLCQGMVLKDGAKMSKSKGNTVDPEALIARYGADTVRLFMMFAAPPEQTLEWSDEGVA
                     GASRFLKRLWKAVHAHLAEGKVAGDIDVAALDDEQRALRCKTHETIAKVSDDYGRRQT
                     FNTAIAAVMELLNEIGRRAERSSPAGLAVEREALEAAVLLLAPVVPHAAHALWLALGH
                     DTPVIDTPWPLADARAMQRSHIELVVQVNGKLRGRVSAPADAAREELERLALADEHVQ
                     RFIDGKPVRKIIVVPGKLVNVVLG"
     gene            10180..>10517
                     /locus_tag="PLW13_10445"
     CDS             10180..>10517
                     /locus_tag="PLW13_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY70840.1"
                     /translation="MPVIDNSKLEILELPGLRHQTIAGHKQGATTMEVWSQTMAPGAA
                     TPVHRHACEEVILVLSGHGECIVDGQAHSFGPNSTLILEPDVVHQLVNTSGEEMRLVA
                     ALGMAPVRVRT"
BASE COUNT         1550 a         3625 c         3612 g         1630 t
ORIGIN      
        1 tgttcggtga tgccgcctac gaagtcatcc cggtctatgg cgggcgcgtg ttccgccccg
       61 cgcagcactt cgagcggctg cgggccagcc tcgcggtgct cggcatcgcg ccgccgcacg
      121 acgccgccgg ctggagcgcc atcattgccc gcctgctggc cgacgccggg ccgcgtgaac
      181 actcgctcta catccacgtc agccgcggcg tggacgcggg tcgcgacgcg gcgcacttca
      241 ccggcctgac gcccacggtg ctggccatgg tcgagccggt cacgctggac cgcgccgtgc
      301 tgcgccaggg catccgcgcc gcgctgctcg aggatacgcg ctggcacaac tgccacgtga
      361 agtcgacctc gctgctcggc aacctgctgc tgcgccgccg cgcgctcgat gacggagcgg
      421 ccgaggcgct gctgcatcgc aacggcgagc tgaccgaggg cacgtcctcg aacgtgttcg
      481 cggtgctcgg cggcgagttg cgcaccgcgc cggcggaccg acgcatcctg cgcggcatca
      541 cgcgcgacgt ggtgatcgag ctggccggcg cctgcgccat accggtcgcc gagcgtgcac
      601 cggcgctgac ggagctcgcg caggccgcgg aactgttcat ctgcagctcc acgcgcgaga
      661 tcgtgccggt taccgtgctc gacggccgcc ccgtcggcga cggtgccccg gggccgcaga
      721 cacgccggct gatcgcagcc ttcgacgact tcaagcgcgc gttcatggag gcggcatgag
      781 cgatatcgag gcgccgcgca tcgagtttcc ctgcgactac ccggtcaaga tcatcggcga
      841 cgcagcggcc gatttccgcg ccgtggtcga ggatatcgtg gagcgccacg ccggcggcta
      901 cgaccgggcg ctcaccaccg agcgctccag cgccaatggc cgcttcgtgt cgctgaccgt
      961 caccatcgtc gccacgggac cggcgcagtt gcagtccctg ttcgcggacc tgaaggccac
     1021 cggccgggtg cacatggtgc tgtgaacatc gcggcggcgc acaccaccct gctcgaggtc
     1081 gcccgtttgg accgggtcga ttacgcgctc gcctggcgcg caatgcaggc ctacaccgac
     1141 acccgcacgc cgcagagccc ggaccgcatc tggctgctgc agcacccctg cgtgttcacg
     1201 ttggggcagg ccggcaaacg cgagcacctg cgcgacaccg gtgcgacccc ggtgatccac
     1261 gtcgaccgcg ggggccaggt gacctggcac gggccggggc agtgcgtggc ctacctgatg
     1321 ctcgacctgc gccggatggg cctgggcgtg cgcgagctgg tcgagcgcat cgaggcggcg
     1381 atcatcgcga cgctgggcca cttcggcatc gagggggtgg cgcgccgcga cgcgcccggt
     1441 gtttatgtcg atggcgcgaa gatcgcggcg ctcggcctgc gcgtgcgacg cggctgctgc
     1501 tttcacgggc tcgccctgaa catcgatccg gacctcggag cgttcgcgcg catcgatccc
     1561 tgcggtcacc ccggaatggc cgtgacgcgc atcgcggacc tggtgccggt gggcaccgac
     1621 gtgtcgcgcg cgcgggtcga ggcgctgctg ctcggcgaac tgctggcggt gttcggttac
     1681 gacccggcgc gcgtgcgcga gtgcggcgcc acggagctcc cggcgccatg agcccggtgc
     1741 attccgtcgc ggcgcgtggt gcccggcgcg catccgggcg cgctgttccc atgatccgtg
     1801 cctgcggcgt tgtcgtaccg cgggccgcca ctcccgggcc ctacgtcccg ccaaggaatc
     1861 caggatgaaa cgcagccttt ccctcgccac gctctgtgtc gccgcgctct tcggcggatt
     1921 cgcgcacggc gcgccctccg catcgcccta tgccaacgcc agcggcatcg accgcggcgg
     1981 cttcgatgac aaggtgcgcc cgcaggacga cttcttcgac tacgtgaacg gcggctggat
     2041 cgccgccacc gcgatcccgc cggaccgcgc ctggtggggc gtggtgccgg aactgcgcgc
     2101 ggagagcgag gcgcgccagc gcgcgatcat cgaggaaatg gccgcgcgca gcgacctagg
     2161 gccggattcc gtggcgcgca agatcggtgc cttcttcgcc agcctcaccg accagtccct
     2221 ggtcgagggc aagggcgtgg caccgatcga gggcacgctg gcgcgcatcg ccgcgatcga
     2281 ttcgaccgaa tccctggcgg cggccttcgg cgccgcgcaa ttgcttggca tcgacgcgcc
     2341 gctcgccgtc ggcgtggtgc aggacccggg cgccgcggac cgttatgtgc cctatatctg
     2401 gcagagcggc attgcgctgc cggatcgcga ctactacctg cgcgacgacg agaagttccg
     2461 caagatccgc gcggcctatc cgggctacat cgcgaagctg ctgggactcg gcggcttcgg
     2521 cggcacgccg gaacaggggc aggcggtgtt cgcgatcgag cagcgcctcg ccgagctgca
     2581 ctggccggcg caagaaaacc gcgacatgaa gaagctctac aacatggtgc ccgccgcgca
     2641 gctgacggcg gtggcgcccg atttcgcgtg gtcgcagtac atcgacgcgg ccgggctcgg
     2701 cgcgcggccc gagctgatgg cggcgcagct gagctacgtg aagcagatcg gcgcgatcgt
     2761 gggcgagttc ccgctcgagg cgtggaagga ctacctgcgt taccacgcgc tgaactccgc
     2821 cgcgccctgg ctctcgggcg agttcgagca ggcgaacttc gaattcatgc agcgcgaggt
     2881 gctggggttg acggaactgg cgccggaatg gaagcgcgcg gtgcgcgcca tcgacgcgct
     2941 ggccggcgag gccgtgggac aggtctacgt cgagcgctac ttccccgcag gccacaagca
     3001 ggcgatgctc gggctggtcg acgacctgat ggaggccttc cgcaccggca tcaccgagct
     3061 ggagtggatg agcccggaga cgcgggcgaa agccgaggcc aaacgcgcgc ggctcgccac
     3121 caagatcggc taccccgaca agtggcgcga ctattcgggg ctggaggtcc gcgccgacga
     3181 cccgctcggc aacctcgcgc gcgcctcggc gttcgagtac cgccacgaac tcgcgaagct
     3241 cgaccagccg atcgatcgcg ccgagtggtc gatgacgccg cagaccgtca acgcctacca
     3301 ccagccgttc atgaacgaga tcgtgttccc ggcgggcttc ctgcagccgc cgaacttcaa
     3361 catggccgcc gacccggcgg tgaactacgg cgccatcggc tacgtgatcg gtcacgagat
     3421 cgggcatgcc ttcgacgaca agggccgcgc cttcgatcct tacggcaaac tcaacgactg
     3481 gtggagcgcc gcggatgccg agcacttcga gcaggccgcc gcgaagctcg tcgagcagta
     3541 caacgccttc cgcccgctgc cggacatgtc gatcaacggc aagctcacgc tgggcgagaa
     3601 catcgcggat ctgaccggca tcaccattgc ctaccgcgcc taccgggcgt cgctgggcgg
     3661 caaggaagca ccggtgatcg acggcctgag cggcgaccag cgcttcttca tcggcttcgc
     3721 gcaggccaac cgcgccagca tgcgcgagga aatgcttcgc tcgatgctgg tcagcgatcc
     3781 gcactccccg gcgcgctatc gcgtgctcgg ggtgctgcgc aacttcacgc ccttctacgc
     3841 cgccttcggc gtgaaggaag gcgacggcat gtacctgccg cccgacgagc gcgtgaagat
     3901 ctggtaaatt ggcgccgtcg gatgatgatc ctgtaggagc gggccatgcc cgcgaacggt
     3961 cgcgggcatg gcccgctcct acgcctgttc cttgtagagc cgtgggggca cacccgtgac
     4021 agaacaatcg acttcctcgc gccccgcgcc acgcaaggcc gtgcagggcg agaagctgcg
     4081 cggcgccgag aaggtcgcgc gtatcccggt gaaggtcatt cccaccgtcg agttgccgcg
     4141 caagccggac tggatccgcg tgcgcgtgcc ggcctcgccc gaggtcacgc gcatcaagga
     4201 gatcctgcgc cgccacaagc tcgcctcggt ctgcgaggaa gcggcctgcc cgaacctcgg
     4261 ggaatgcttc agcggtggca ccgcgacctt catgatcatg ggcgagatct gcacgcgacg
     4321 ctgcccgttc tgcgatgtcg cccacgggcg tccgaacccg ctcgatgccg gggagccgcg
     4381 cgggctggcc gaggcgatcg cggaaatgaa gctgcgctac gtggtcgtga cctcggttga
     4441 ccgcgacgac ctgcacgacg gcggagcagc gcatttcgcg gcctgcatcg gcgagacgcg
     4501 ggccctgaat ccgggcatcc gcgtcgaggt gctggtgccc gatttccgcg gccgcatgga
     4561 cgttgcgctc gacatcctcg agcgcgagcc gccggatgtg ttcaaccaca atctcgagac
     4621 cgtgccgcgc ctctacctgc agtcgcgtcc cggctcggat tacgcgtggt cgctggacct
     4681 gctgcggcag tacaaacagc gttgccccga ggtgctcacg aagtccggac tgatgctcgg
     4741 tctcggggaa accaacgagg aagtggtcga ggtgatgcgc gacctgcgtg cccacgacgt
     4801 ggacatgctg acgctcggac agtacctgca gcccagccgc aaccacctgc cggtcgaccg
     4861 cttcgtgccg ccggcggagt tcgaggaact cgggcgcatc gcgcaatcgc tgggctttcg
     4921 cagcgtggcg agcgcgccgc tggtgcgctc ctcctatcac gccgacaagc aggtggacct
     4981 cagcctcctg aaatcctgat tccggggtgc gggtccggct tcatccggat acgcagcgcc
     5041 tttttgtggg tccggattca tccggacaag ggttcgacgc aagccgaatc cgcagaggca
     5101 cggtcacgcc ggcaaccggg aggagacccc atgtacgaac tctacatcgc caacaagaac
     5161 tattcgtcgt ggtcgctgcg cccgtgggta ctgatgcgcg aactccgtat cgccttcgac
     5221 gagcgcctgg tccccttcgc gccgggttcg aacttcgagc gcttccgcgc gttctcgccc
     5281 acgggcaagg tgccgtgcct gcatgacggc gcaacggtgg tgtgggattc gctcgcgatc
     5341 gcggaatacc tcgccgaacg ccacgccggc gtctggccgt ccgatgcgcg cgcccgcgct
     5401 tgggcgcgct cggccgcggc cgagatgcat tcgggcttcg gtgcgctgcg ccagcnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgggc gcgctcggcc gcggccgaga
     5581 tgcattcggg cttcggtgcg ctgcgccagc gcgcgagcat gagctgcggg cagcgcgtgc
     5641 ggttgaagga gctccccgcg gacctgcgcg ccgacctcgc gcgtctcgag gccttgtgga
     5701 ccgaaggcct ggcccgtttc ggcggaccct tcctggcggg agcggccttc ggcgcggtgg
     5761 acgcgttcta cgcgccggtg gcgtttcgct tgcagaccta tgccctgccg ctcgcagagc
     5821 ccgcggcggc ctacgcgatg cggctgctcg ctctggcggc gatgcgcgac tggtacgccg
     5881 ccgcgctcgc cgaaccctgg cgcgacgagc cgcacgaagc ggatatcgcg gcagcgggga
     5941 cgatcacgca ggatttgcgg cgcgcgccgt aggtgccatt catggcgtca gcagcgcccg
     6001 gccaggcacc agtcggcatg aatgccgacc tacgggcggg ggttggcggg acggcctgcg
     6061 cgcaccacca gctccagcag cgcgtcccag gcatcgcgct tgtccagtcc cttgatgcag
     6121 cgatccacct cggcggccag tcgcagcagt tgctgcaggc cctgatgccc gaggcgacgg
     6181 aatgcggcct cgctgtaggg cttgccacgc tcggctttcg tggcctgctc gctgccttcc
     6241 ccggtggcac ggcggcgccc gatacgcagc aggcgacgga actcctcggc gagcgcccac
     6301 aacacgatgg ccgcctcggt gccctcggca cgcagtcccg tgagcgtgcg caccgcggcg
     6361 gcgcgatcgc cctcgagcgc gcgatcgcag agatcgtaga ccgagtagcg cgccgagtcg
     6421 ttcaccagcg ccgccagcgt ggcggcgtcc agcggaccgg gttccgccag cagcgccagc
     6481 ttgtcgatct cctggcgcgc ggcgagcagg ttgccttcga cgcgctcggc cagcagcgcc
     6541 accgcgtcgg ggctgggctt gaatcccgcg gcgcgcaggc gctggccgat ccagcccggc
     6601 agttcgttgc gcctgagcgg ccaggccctg accgtggcgc cggcctcgtc cagcgcgcgg
     6661 aaccacgcgg atttttccac gtcgcggtcg agcttgccgc cgagcaccag caccacgttg
     6721 tcggatgccg gctgcgccgc gagctcgcgc agcatcgcgc tgccctcggc gcccagcttg
     6781 gcgccggcga ggcgcacctc gatcagcttg cggtcgccga acagcgacag accgctgcac
     6841 tccgcgctga ggcggctcca gtcgaagccg gcgtccacgt cgaacgcctg ccgctccccg
     6901 atgccgccgt tgcggcaggc cgcacgcagc gcgtcggcgc attcctgcac cagcagcggc
     6961 tcgtcgccga agagcaggta gaacggaaac agccgcgcct tgagcctggc ggggaaatcc
     7021 tcgacgcgga tacgcacggt ggtgcctagg gcgccaggcc ggcttgcgcg cggcgcagct
     7081 ggcgcaagac ctggcccacg acgttgtcca gcaactcctg gtagatgagg ttctcctcgt
     7141 cctgcttggc gatgatgctg ttggggtcga aggcgtagat gcggttcgcg ctggcggtgc
     7201 gcggcccgaa caccacctcg ccgtcggcgt tgcgaagatc gaaggccacc gagacgcgca
     7261 tctcctgctc ggcgctgcgc gcgtcgcggt cgagcgagat cttgcgcgac acgcgctgct
     7321 ctgtgccgat gtgcagcgac gggccggctc cggcgacatc tacgagctcc acgccggcgc
     7381 cctgcagcgc gcggcgcagc ttcagggtga gcgcggcgtt gggctgctcc gtcacgaggc
     7441 gcagctggcg catctgcagc gggacttcct gcgagccgcg cggatgccag ccgcaggcgg
     7501 ccagggtcag caccgtgatc agggcgagcg agacgctacg gagcagagtg ttgcgcatgg
     7561 cggtctaccc gagcacgacg ttcacgagct tgcccggcac cacgatgatc ttgcgcaccg
     7621 gcttgccgtc gatgaagcgc tgcacgtgct cgtcggcgag cgccaggcgt tcgagctcct
     7681 cgcgcgcggc gtcagccggc gcgctcacgc gcccgcgcag cttgccgttc acctggacca
     7741 ccagctcgat gtggctgcgt tgcatcgccc tagcatcggc cagcggccac ggtgtgtcga
     7801 tcaccggcgt gtcgtggccc agcgcgagcc acagcgcgtg cgcggcgtgc ggcaccaccg
     7861 gtgccagcag cagcaccgcc gcttcgagcg cctcgcgctc caccgcgagt cccgccggcg
     7921 agctgcgctc agcgcgcctg ccgatctcgt tgagcagttc catcacggcc gcgatggcgg
     7981 tgttgaaggt ctggcggcgc ccatagtcgt cgctgacctt cgcgatggtc tcgtgggtct
     8041 tgcagcgcag tgcgcgctgt tcgtcgtcga gtgcggcgac atcgatatcg cccgccacct
     8101 tgccctcggc caggtgcgcg tgaacagcct tccacaaccg cttgaggaag cggctggcgc
     8161 cggcgacgcc ctcgtcggac cactcgagcg tctgctcggg cggcgcggcg aacatcatga
     8221 acagccgcac ggtgtcggcg ccgtagcgcg cgatcagcgc ctcgggatcc accgtgttgc
     8281 ccttggactt ggacatcttg gcgccgtcct tcagcaccat gccctggcac agcagccgcg
     8341 tgaacggctc gtcgctggtc accagcccct cgtcacgcat cagcttgtgg aagaagcgcg
     8401 agtacagcag gtgcaggatc gcgtgctcga taccgccgat gtactggtcg accggcagcc
     8461 agtagttcgc gcgcgcgggg tcgagcatcg cggtctcgag gtcggggcag gtgaagcgcg
     8521 cgtagtacca cgacgattcc atgaaggtgt cgaaggtgtc ggtctcgtgc tccacttcct
     8581 ggccggccag cgtgctcctg cgccacgccg gattggcctt gatcggcgac tgcacgccgc
     8641 tcatctcgac gtcctcgggc agcagcaccg gcaggcgatc cgcgggcacc gggatctcgc
     8701 cgccgtcggc gagatagagc atcgggatcg gcgcgcccca gtagcgctgg cgcgacacgc
     8761 cccagtcgcg caggcggtag ttcaacgtca cgcggccatt gccgttggcg gccaggcgcg
     8821 cggcgatcgc atcgaaggcc tgctcgaatc cgagcccgtc gaactcgccg gaatttagca
     8881 gccgtccctt ctcggtgaac gcggcgctcg cgaggtcgca gggcgtggcc ggatccagcg
     8941 gcgcgatcac ctgctcaatc ggcagcccgt aggcgcgcgc gaactcccag tcgcgctggt
     9001 cgtggccggg cacgctcatc acggcgccgg tgccgtactc catcagcacg aagttcgcga
     9061 cccagatcgg cacctcgcgt ccgctgagcg ggtggagggc cttcaatccg gtatcgaggc
     9121 ccttcttcgc catggtggcc atgtcggcct cggcaacgga gctgcgccgg cactcggcga
     9181 tgaagtccgc gagctccggg cggccttcgg ccagcgcctt cgccagcgga tgctcgccgg
     9241 cgatcgcgag ataggtcacg ccgagcaggg tgtcgggacg ggtggtgtag atcggcacct
     9301 cggtgatgcc cgccagcggc gtggccaggg ggaagctcat ctcgatgccg cggctcttgc
     9361 cgatccagtt gcgctgcatc gtcttgacct gttcgggcca gtgctcgagc ttgtccaggt
     9421 cgttcagcag ttcctcggcg taatccgtga tgcggatgaa ccactgcggg atctcgcgcc
     9481 gctccaccgg cgtgtcgcag cgccagcagc agccctcgat gacctgctcg ttggcgagca
     9541 cggtggcgtc cttcgggcac cagttcaccg ccgagacttt cttgtacgcg aggcccttct
     9601 cgtagagacg cgtgaagaac cactgctccc agcggtaata cgcgggttgg caggtggcga
     9661 gctcgcggct ccagtcgtag gcgaagccga ggcgcttgag ctgcccgcgc atgtagtcga
     9721 tgttggcgtg ggtccagcgc gccggcgcgg tgttgttctg gatcgcggcg ttctcggccg
     9781 gcaggccgaa ggcgtcccag cccatcggct gcagcacgtt gcgcccctgc atgcggtgaa
     9841 agcgcgcgat cacgtcgccc agcgtgtaat tgcgcacgtg ccccatgtgc aggcgtccgc
     9901 tcgggtacgg gaacatcgac aggcagtaga atttctcgcg cgcgggatct tcggtgacga
     9961 cgaagctgcg attgtgctcc cagaatgcct gcgcatcggc ctcggtgcgt tcggcgtcgt
    10021 agagttcttc catggtctgg gcgggttctg gcggaaaaaa ggcgcacagg atagcgcacg
    10081 gggctcggcg cgggccagca cggggtagcc gcagggttgc acactcactg ccccgtaggt
    10141 aaggattcgc tccgcctgca caattacccg ggaggcgaca tgccggtgat cgacaacagc
    10201 aaactggaaa tcctggaact gcccggcctg cgccaccaga cgatcgccgg ccacaagcag
    10261 ggcgcgacga cgatggaggt ctggtcgcag acgatggcgc ccggcgcggc aacgccggtg
    10321 caccggcacg cttgcgagga agtcatcctc gtgctctcgg gccacggtga atgcatagtg
    10381 gatggccagg cgcattcctt cggccccaac tcgacgctga tcctcgagcc ggacgtggtg
    10441 caccaactcg tgaacacctc gggcgaggag atgcgcctgg tcgccgcgct cggcatggcg
    10501 ccggtgcggg tcaggac
//
LOCUS       DAORUQ010000111        10466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6703_length_10466_cov_5.344059, whole genome shotgun sequence.
ACCESSION   DAORUQ010000111 DAORUQ010000000
VERSION     DAORUQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6703_length_10466_cov_5.344059"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLW13_10450"
     CDS             <1..243
                     /locus_tag="PLW13_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70841.1"
                     /translation="AEGIAGRLRAAGLWSEAPVKWVAEDHRASYRAQLALVAALRYRH
                     QGTELLATRFDHPKFPSDAARWAAWLGVLDTALVRA"
     gene            291..1163
                     /gene="nudC"
                     /locus_tag="PLW13_10455"
     CDS             291..1163
                     /gene="nudC"
                     /locus_tag="PLW13_10455"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HQY70842.1"
                     /translation="MELKPGFTGATLDRADALRTNDERLAAARADPRARLLGLNGLEP
                     LTGGDGRLLWRPMHEAAAGNELVLLGLDGGEPRFAELRVEEADSVNRSMAVMQALARL
                     DAADAALYGGARSVIDWHYRHLFCARCGAATALFRAGWGRKCARCNTEHFPRVDPVVI
                     MLAEFGGRVLLGRQPMFPPKFFSALAGFVEPGESLEEAVRRELLEEAGIRTGAVRYVV
                     SQPWPFPSSLMIGCVAEALDEAITLDTTELQDAMWVGRAAVEAALAGEAEAPFVPPPP
                     VALAHSLLRAWLAS"
     gene            complement(1170..3386)
                     /locus_tag="PLW13_10460"
     CDS             complement(1170..3386)
                     /locus_tag="PLW13_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY70843.1"
                     /translation="MIKRMLVNALLALATAAALALPTGAAGAEVPRVGLVLAGGGARG
                     LAHIGVIKYLEEHGIRVDAIAGTSMGAVVGGMYASGLDAAAMEEIATTLDWKYALDDT
                     TPRDQLSFRRKQQDLDFLVRAKLRFRDGKLRLPMGAIEGQHLNLVLHDIVGHVSRIND
                     FDALPIPFRAVATDIETGDPVILARGDLAVSMRASMSIPGIFSPVDLGGKLLVDGGIA
                     NNVPVDVVQAMGVERLIVIDVGTPLYDRGKLENVLSLMDQLTTIMTRKNSEQQLARMG
                     PGDILVSPALDEAGVLTMGFDKAELAIRLGYEAAAAMGPQLAALAAPQAPHPAERPRL
                     RLPRIDEIRVDSNAPVSKALLRNMIKQPLGKKLDREQMERDIAEIYGLDEFSRVDYDV
                     EREDERYVLTLRATAHPAGVNYLRLGMSWDQDSRGSSEFGLRASWLQKGINSLGAEWY
                     TVGQLGGRSRFGTEFHQPLDVGRRYFVDARYDYQQRLINISESGDVRARALVDDHRLE
                     LAPGMHFANLASVRAGGYLGTANTDIQIGSPLLSSNSDDDGGFFTELRYDSLDRAFFP
                     RRGLRVVSRYDNGLESWGAEDYEAWSTTGVLSRSWGRHTVNATARWSQLDLDADETVL
                     TLPSQVFTLGGFLSLSGYTRDSLAGNYLGLANLVYYRRLNQQSILPTDLPVYAGASIE
                     AGNTWFYKEDVALDDLLYAGSLFLGVDSPLGPVYLGVGFGENDQRALYLQIGQVFD"
     gene            3538..3822
                     /locus_tag="PLW13_10465"
     CDS             3538..3822
                     /locus_tag="PLW13_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70844.1"
                     /translation="MNPLAATRAIALALILCLLALDGAHHVHVPDAGTAQTDCSLCAA
                     GSAAKAPASGVVVPVIPFHRVPVAARALALVARAPAQHVCIRGPPATSVA"
     gene            3906..5990
                     /locus_tag="PLW13_10470"
     CDS             3906..5990
                     /locus_tag="PLW13_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70845.1"
                     /translation="MQELRPHAARLGAAALLASAVAAHAADPRLEEIIVTAPMHKGEA
                     ETVHPTSLIAGNELRRRIAATLGETLKQEPGVAFSSFGPGVGQPVIRGQGAPRVLVLQ
                     NSMPVGDAANTSADHVNATEPVLADRIEVLRGPATLLYGSGAIGGVVNVIDNRVPATV
                     PQAPGGALEYRHGSNAGSDVLAGRLDAGAGDVALHLDGFGRESGDVEIPGNALRDGGG
                     SEGSIENSEAQASGATAGASWVFGRGFLGLAVSRLDNEYGVPPGAHDHAEHDGHDEDG
                     DHALALEAAGGEEEAGAGDVRIDLQQTRYDLRAEFADPLRGVELLRSYLAYSDYRHDE
                     IESGVTGTRYGNEAWDGRIEAVHRSSDALHGAIGLQLHSRKFTAAGEEAFVPDADGES
                     WGVFLVEDLHVGDVVYEFGLRAGRDSHDPARGDARDFGTVSGSVSALWSLDDVQTLKF
                     ALSGAGRAPAMEELYSEGVHVATRSYELGDESLDEERSLNADLGYHLHQGAVDFRIDA
                     FYNRYDDYIFQRATGAFFNPELALLEPVCSADREDECLEVLQWAAAEADFYGLESELR
                     LELPRSFTLAIFGDHVRGEFDDGGDVPRVPPGRLGAELAWQHGGWDLAARVTEVLEQD
                     RTGEGERSVPGYTLLAARAEYVFAAGGGEWTLFLRGENLLDEEIRNASSLLRDVAPEA
                     GLNIEAGVRLAF"
     gene            6109..7974
                     /locus_tag="PLW13_10475"
     CDS             6109..7974
                     /locus_tag="PLW13_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70846.1"
                     /translation="MQSEHFRPCLAVALIVLMIAAAPAGVRAAASDGLEASYRFLVSD
                     RREQGGALFTATAGTLNGLQLPLSYRDSADYWGDYVCRLPGHDCAVLDVYDDAAYTLR
                     PPRGAAGELQMERVNVHNGANIYDAATWQIAVMLGSVVLRLPGPAPQAAWALASGQNA
                     LLAAGHDAEAPHFAPGSNRAVTRGELFRYNGRAITDSRHAFAYRMPGRRWLADDPFID
                     TRHAAWIRTTALPAGNSDYRPGKVSWSDWKAFTGENAWAFLIGPLQAAHIHFVRHLQQ
                     PCVPFGEPAVQAALQLLPAFAAMQAPIGGVHYAPSGTLRNRLDEPVDPREIVVENNVS
                     LYAGLRILEATLRAQLQHDKALAAADRASIDRALALISVMIHGGRHPDGAPTAGMLAF
                     LRDHAWRDGEFVQGGLALGPQEGNAWVPTTSPRAVDANTWGVLALGPGTVDGWFGFGA
                     ALRNWQQLKRWGGYGEGTTLWGVGYSELDGNGIDANGMYRAGILSSEWTAGAITLVRS
                     LAAHYGRIAPQAPQYAEARGWLEELRRDEQSMLAGLGRLRLDAYRATPFAGKPPDYER
                     LLPRQGDTQPYVYASRRYAIPFGWYANPLPSTCATAWVIMLAAGFDPFGYGGVPN"
     gene            8020..>10466
                     /locus_tag="PLW13_10480"
     CDS             8020..>10466
                     /locus_tag="PLW13_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQY70847.1"
                     /translation="MLQHNVTLDDKYTLASGRAYMTGIEALVRLTMLQRERDRARGLN
                     TAGFVSGYRGSPLGGLDQALWKARPHLARHDVVFRPGVNEDLAMTAVRGTQEVTLFPG
                     ARVDGVYAMWYGKGPGLDRSMDVLKHANAVGTSRHGGVLAVVGDDHACKSSTLPHQCE
                     HAFMGASAPVLNPANVQEILDLGLLGWELSRYCGCWVGFKVITENCDAAASVDIDPHR
                     ISVKIPEEFRLPPGGVHARLHDKPLAQEERLNLYKIYAAREFARVNHLNYVAIDSPRP
                     RLGIVSTGKSWLDVLQALEDLGIDCDQAAALGIRVFKVGMPWPLDPVATHAFAEGLEE
                     ILVVEEKRSIIEDQLTGQLYNWPVERRPRVIGEFDEHGRSLLPNLAELTPAMVARAIA
                     SRIGRYYQGEDMQRRLEFLDRKERSLATPRAIIERTPHYCSGCPHNTSTVVPGGSRAG
                     GGIGCHYMVTWMDRNTDTFTQMGGEGATWIGQTPFTDTAHVFQNLGDGTYFHSGILAI
                     RAAVAAGVNITYKILYNDAVAMTGGQPIDGQLTVAQLIEQLRGEGVKRIALVSDEPEK
                     YPRALHHAAGVTIHHRDELDAVQREFREICGTTAIVYDQTCAAEKRRRRKKGAYPDPE
                     RRVFINELVCEGCGDCGVQSNCLSVLPKETAFGRKRMIDQSACNKDYSCLKGFCPSFV
                     TVEGGRLRKSRAVLAEVALPTLPEPVLPALERPWNTVVTGIGGTGVLTVSAILGMAAH
                     LEGKGCTVLNQSGLAQKFGPVVSHVRIGRRQADIHAVRVAAGDADLLLGCDLVVSAAE
                     DPLAKVNIERSHAVVND"
BASE COUNT         1537 a         3586 c         3745 g         1598 t
ORIGIN      
        1 gccgagggca tcgccgggcg gctgcgcgcg gccgggctgt ggtccgaggc gccggtgaag
       61 tgggtcgccg aggatcaccg cgccagttac cgcgcgcagc tcgcgctggt cgcggcgctg
      121 cgctaccgtc accagggcac tgaactgctg gccacgcgtt tcgatcaccc gaagttccct
      181 tccgacgccg cgcgatgggc ggcatggctg ggcgtgctcg ataccgccct ggtgcgcgcc
      241 tgagcgtcgc gcggcacgcg aacaccgatc ttcgaacgga tcaccagcac atggaactca
      301 aacccggatt caccggcgcg acgctggatc gcgccgacgc acttcgcacc aatgacgaga
      361 ggctcgccgc ggccagggcc gatccgcgtg cgcgcctgct tggcctgaac gggctggagc
      421 cgctgaccgg cggcgacggc cggctgctgt ggcgcccgat gcacgaagcc gccgcaggta
      481 acgagctcgt gctgctcggc ctggacggcg gcgagccgcg ctttgccgag ctcagggtcg
      541 aggaggccga cagcgtgaac cgctcgatgg ccgtgatgca ggcgctggcg cgcctggacg
      601 ccgcggacgc cgcgctctac ggcggcgcgc gcagcgtcat cgactggcac taccggcacc
      661 tgttctgcgc ccgctgtggc gccgcgaccg cgctgtttcg cgccggctgg ggccgcaagt
      721 gcgcgcgttg caacaccgag cacttcccgc gcgttgaccc ggtggtgatc atgctcgcgg
      781 agttcggggg ccgcgtgctg ctcgggcgcc agccgatgtt cccgccgaag ttcttctcgg
      841 cgctcgcggg cttcgtggag ccgggcgaat cgctggaaga ggcggtgcgc cgcgagctcc
      901 tcgaggaggc gggcatccgc actggcgcgg tgcgctacgt ggtgagccag ccgtggccgt
      961 tcccctcgtc gctgatgatc ggctgcgtgg ccgaggcgct ggacgaggcc atcacgctcg
     1021 acaccacgga gctgcaggac gcgatgtggg tcggtcgtgc cgccgtggag gccgcgctgg
     1081 caggcgaggc cgaggcgccc ttcgtaccac cgccgccggt cgcgctggcg cactcgctgc
     1141 tgcgcgcctg gctggcgtcc tgagcggcgt cagtcgaaga cctgcccgat ctgcaggtag
     1201 agcgcgcgct ggtcgttctc gccgaaaccg acgccgagat acaccgggcc gagcggcgag
     1261 tccacgccga ggaacaggct gccagcgtac agcaggtcgt cgagcgcgac gtcctctttg
     1321 tagaaccagg tgttgcccgc ctcgatgctc gcgcccgcgt aaaccggcag gtccgtgggc
     1381 aggatcgatt gctggttgag ccggcggtag tacaccaggt tcgccaggcc caggtagttg
     1441 cccgcgagcg aatcgcgcgt gtaccccgac agcgacagga agccgccgag cgtgaatacc
     1501 tgtgagggca gggtgagcac cgtctcgtcc gcatcgagat cgagttgcga ccagcgcgcc
     1561 gtcgcgttca ccgtgtggcg cccccagctg cgcgagagca cgccggtcgt gctccaggct
     1621 tcgtagtctt ccgcgcccca ggattccagg ccgttgtcgt agcggctgac cacgcgcagg
     1681 ccccggcgcg ggaagaaggc gcggtcgagc gagtcgtagc gcagctccgt gaaaaagccg
     1741 ccatcgtcgt cgctgttcga gctgagcagc ggcgagccga tctggatgtc ggtattcgcg
     1801 gtcccgaggt acccgccggc gcgcaccgag gcgaggttgg cgaaatgcat gcccggcgcc
     1861 agctccaggc gatggtcgtc gaccagcgcg cgggcgcgca cgtcgccgct ttcggaaatg
     1921 ttgatcagcc gctgctggta gtcgtacctg gcgtcgacga agtaccgccg tcccacgtcg
     1981 agcggctggt ggaactccgt gccgaagcgc gagcgaccgc cgagctgccc cacggtgtac
     2041 cactccgcgc cgagggagtt gatgcctttc tgcagccagc tcgcgcgcag cccgaactcg
     2101 ctgctgccgc ggctgtcctg gtcccagctc atgccgagcc tcaggtagtt gacgccggcg
     2161 gggtgcgcgg tggcgcgcag cgtcagcacg taacgttcgt cctcgcgctc cacgtcgtaa
     2221 tccacgcgcg agaactcgtc caggccgtag atctcggcga tgtcgcgctc catctgctcg
     2281 cggtcgagct tcttgccgag cggttgcttg atcatgttgc gcagcagcgc cttcgatacc
     2341 ggcgcattgc tgtcgacgcg gatctcgtcg atgcgcggca gtcgcaggcg cgggcgctcg
     2401 gccgggtgcg gcgcctgcgg cgcggcgagt gccgcgagct gcggccccat tgccgcggca
     2461 gcctcgtagc cgagccggat cgcgagctcg gccttgtcga agcccatcgt cagcacgccg
     2521 gcctcgtcga gcgcgggtga taccaggatg tccccgggtc ccatgcgtgc gagctgctgc
     2581 tcggagttct tgcgcgtcat gatcgtggtg agctggtcca tcagcgacag cacgttctcg
     2641 agcttcccgc ggtcgtagag cggcgtgccc acgtcgatga cgatcaggcg ctcgaccccc
     2701 atcgcctgca ccacgtcgac cggcacgttg ttggcgatac cgccgtcgac cagcagcttg
     2761 ccgccgagat ccacgggcga gaagatgccc ggaatcgaca tgctcgcgcg catcgacacg
     2821 gcgagatcgc ctcgcgcgag aatcaccggg tcgccggtct cgatgtcggt ggccacggcg
     2881 cggaagggga tcggcaacgc atcgaagtcg ttgatccgcg acacgtggcc gacgatgtcg
     2941 tgcagcacga ggttcaggtg ctggccctcg atcgcgccca tcggcaggcg caacttgccg
     3001 tcgcgaaagc gcagcttggc gcgcaccagg aagtcgagat cctgctgctt gcgccggaac
     3061 gagagctggt cgcgcggcgt ggtgtcgtcg agggcgtact tccagtccag cgtggttgcg
     3121 atctcctcca ttgccgcggc gtcgagtccc gaggcataca tcccgcccac gacggcgccc
     3181 atgctggtgc ccgcgatggc atcgacgcgg atgccgtgct cctcgaggta cttgatcacg
     3241 ccgatgtgcg ccagtccgcg cgcgccgccg ccggcgagca cgagaccgac gcgcggcacc
     3301 tcggcgccag cggcgcccgt cggcagggcc agcgccgctg ccgtggcgag cgccagcagg
     3361 gcattgacga gcatgcgctt gatcatcccg atgtcctcat ggtccgggcc ggagggccga
     3421 agcggcattt tccgcagcgg gccggtgccc ggtccacgac aattaccggg catttggagc
     3481 cggtcatgcc gctgccctat aatcgcgccg cctccatccg acggaccccg ctagccgatg
     3541 aacccgctgg cagcgacacg cgccattgcg ctcgcgctga tcctgtgcct gctggcgctg
     3601 gacggggctc atcacgtcca tgtcccggat gccggtaccg cccagaccga ttgttccctg
     3661 tgcgccgcgg gcagtgccgc caaggctccc gcgagcggtg tcgtggtacc ggtcatcccg
     3721 tttcatcgcg tgccggtcgc ggcgcgggcc ctcgcgctcg ttgcccgcgc cccggcgcag
     3781 cacgtctgca tccgcggccc gcccgcaacc tccgtcgcct gaaacctgag cggggccgcg
     3841 aagccccgcg agcacgggcc gtctgccgac ggtccgtatc ctgattgtcc gatggagtag
     3901 tcaccatgca agaattgcgt ccgcacgcgg cgcgcctcgg cgccgctgcc ctgctcgcga
     3961 gcgctgtcgc cgcccatgcc gccgatccgc gcctcgagga gatcatcgtc acggccccga
     4021 tgcacaaggg cgaggccgag accgtgcacc cgaccagcct gattgccggc aacgagctgc
     4081 gccggcgcat cgcagcgacg ctcggcgaga cgctgaagca ggagcccggt gtcgcgttct
     4141 cctccttcgg tccgggcgtg gggcagccgg tgatccgcgg ccagggcgcg ccgcgcgtgc
     4201 tggtgctgca gaacagcatg ccggtgggcg atgccgccaa caccagcgcc gatcatgtca
     4261 atgccaccga gcccgtgctg gccgatcgca tcgaggtgct gcgcgggccg gccacgctgc
     4321 tctacggcag cggtgccatc ggcggcgtgg tcaacgtgat cgacaaccgt gtgccggcca
     4381 cggtgccgca ggcgccgggc ggagccctcg agtaccgcca cggcagcaac gccgggagcg
     4441 atgtgctggc cggtcggctc gatgcgggtg ccggcgacgt ggcgctgcat ctcgacggct
     4501 tcgggcgcga gagtggcgac gtggagattc cgggcaacgc gcttcgcgat ggcggcggca
     4561 gcgagggatc gatcgagaac agcgaggcgc aggcctccgg cgccaccgcc ggcgcgtcgt
     4621 gggtgttcgg tcgcggcttc ctcgggttgg cggtgagccg gctcgacaac gagtacggtg
     4681 tgccgccggg tgcacacgac cacgcggagc acgacgggca cgacgaggac ggtgatcacg
     4741 cgctggcgct ggaggcggcg gggggcgagg aggaggcggg cgccggggac gtgcgcatcg
     4801 atctgcagca gacccgctac gacctgcgcg cggagttcgc cgatccgctg cgcggcgtgg
     4861 agttgttgcg cagctatctc gcgtattccg attatcggca cgacgagatc gagagtggtg
     4921 tcacgggaac ccggtacggc aacgaggcct gggacggccg catcgaggcg gtgcaccgct
     4981 cgagcgatgc gctgcacggc gccatcggcc tgcaactgca ttcgcgcaaa ttcacggccg
     5041 cgggagagga ggccttcgtg ccggacgccg acggcgagtc ctggggcgtg ttcctggtcg
     5101 aggacctgca tgtcggcgac gtggtctacg agttcggcct gcgcgcgggc cgcgacagcc
     5161 acgatccggc gcgcggcgat gcgcgcgatt tcggcaccgt gagcggctcg gtctcggcgc
     5221 tgtggtcgct cgatgacgtg cagacgctga agttcgcgct gtccggcgcc gggcgcgcgc
     5281 ctgcgatgga ggagctctac tccgagggcg tgcacgtggc cacgcgcagc tacgagctcg
     5341 gcgacgagag cctcgacgag gagcgctcgc tgaatgccga cctcggctat cacctgcacc
     5401 agggcgccgt ggatttcagg atcgacgcgt tctacaaccg ctacgacgac tacatcttcc
     5461 agcgcgcgac cggcgcgttc ttcaacccgg agctggcgct gctggagccc gtgtgcagcg
     5521 ccgaccgcga ggacgagtgc ctcgaggtac tgcagtgggc ggcggcggag gcggatttct
     5581 acggcctgga gtccgaactg cgcctggagt tgccgcgcag tttcacgctg gcgattttcg
     5641 gcgatcatgt gcgcggcgag ttcgacgacg gcggcgatgt gccgcgcgtt ccgcccgggc
     5701 gcctgggcgc cgagctggcg tggcagcacg gggggtggga cctggcggcg cgggtgaccg
     5761 aggtgctgga gcaggatcgc accggcgagg gcgagcgttc cgtgccgggc tacacgctgc
     5821 tggcggcacg cgccgaatac gtattcgcgg ccggtggtgg tgagtggacg ctgttcctgc
     5881 gcggcgagaa cctgctcgac gaggagatcc gcaatgccag ctcgctgctg cgcgacgtgg
     5941 cgccggaggc ggggctcaac atcgaggcgg gggtgcggct ggcgttctga gctacccgcg
     6001 cccggccagg gccccgcgcg ctcgcgcgtg catgcgccgg cggggcgccc ccgtgctggc
     6061 gcgggtgcgt gcgttgtgat gcaatcgggc ggggacagga cgcaaaccgt gcaatccgaa
     6121 cacttcaggc cgtgcctcgc agtcgcgctg atcgtgctga tgatcgctgc cgcgccggcc
     6181 ggcgtgcgcg ctgccgctag cgacggactc gaggccagtt accgctttct cgtcagcgat
     6241 cgccgggagc agggtggcgc gctgttcacc gcgacggccg gcacattgaa cgggctgcag
     6301 ctgccgctca gctaccgcga cagcgcggat tactggggcg actacgtgtg ccggctgccc
     6361 ggccacgatt gtgccgtgct cgatgtctat gacgacgccg cctacacgct gaggccgccg
     6421 cgcggtgcgg ccggcgagtt gcagatggag cgcgtcaatg tgcacaacgg cgccaacatc
     6481 tacgatgccg cgacctggca gatcgcggtg atgctcggca gcgtggtgct gcgtctgccg
     6541 gggccggcgc cgcaggcggc ctgggcgctg gccagcgggc agaacgcgct gctcgctgcg
     6601 ggtcacgacg ccgaggcgcc gcatttcgcg ccgggttcga accgtgcggt cacccgggga
     6661 gaactgttcc gttacaacgg ccgggcgatc accgattccc ggcacgcctt cgcctaccgc
     6721 atgccggggc ggcgctggct ggccgatgat ccgttcatcg acacgcgcca cgcggcgtgg
     6781 atccgcacca cggcgctgcc cgctggcaat tccgactacc gcccgggcaa ggtgagctgg
     6841 agcgactgga aagccttcac cggcgagaac gcctgggcct tcctgatcgg gccgctgcag
     6901 gccgcgcata ttcacttcgt gcgccatctc cagcagccct gcgtgccctt tggcgagccg
     6961 gcggtgcagg cggcgctgca gctgctgccc gccttcgccg cgatgcaggc gccgatcggc
     7021 ggtgtgcact acgcgccttc gggcacgctg cgcaaccgcc tcgacgagcc cgtggatccg
     7081 cgcgagatcg tggtcgagaa caacgtttcg ctctatgcgg ggctgagaat cctcgaggcc
     7141 acgctgcgcg cacagttgca gcacgacaag gcgctcgcag ccgcagacag ggcctccatc
     7201 gaccgcgcgc tcgcgctgat cagcgtgatg atccacggcg ggcggcaccc ggacggtgcg
     7261 cccaccgccg ggatgctggc gttcctgcgc gatcacgcgt ggcgcgacgg cgagttcgtg
     7321 cagggcgggc tcgcgctcgg gccgcaggag ggcaatgcct gggtgccgac caccagcccg
     7381 cgcgccgtcg atgccaacac ctggggtgtc ctcgcgctgg gccccgggac tgtcgacggc
     7441 tggttcggtt tcggcgcggc cttgcgcaac tggcagcagc tcaagcgctg gggcggctac
     7501 ggcgagggca ccacgctgtg gggcgtgggc tattccgagc tcgacggcaa cggcatcgat
     7561 gcgaacggga tgtaccgcgc gggtatcctg tcctcggaat ggactgccgg cgccatcacg
     7621 ctggtgcgca gcctggcggc gcactacggc cgcatcgcgc cgcaggcacc gcagtacgcc
     7681 gaggcgcgcg gctggctcga ggaactgcgc cgcgacgagc agtcgatgct cgcgggcctg
     7741 gggcggctgc gtctggatgc gtaccgcgcc acgccttttg cgggcaagcc gccggattac
     7801 gagcgcctgc tgccgcgcca gggggacacg cagccgtatg tctatgccag tcgccgctac
     7861 gcgattccgt tcggctggta tgccaacccg ctgcccagca cctgcgcgac cgcctgggtg
     7921 atcatgctgg ccgcgggctt cgaccccttc ggttacggcg gggtcccgaa ctgagacatc
     7981 atcgctcgcg cataccccga taacagggag cctcgcgcca tgctgcagca caacgtcacc
     8041 ctcgacgaca agtacacgct cgccagcggg cgtgcctaca tgaccggcat cgaggcgctg
     8101 gtgcgcctga cgatgctgca gcgcgagcgc gaccgcgcgc gcggcctgaa caccgcgggc
     8161 ttcgtctcgg gttatcgcgg ctcgccgctc ggtggcctcg accaggcgct gtggaaagcg
     8221 cgaccccatc tcgcgcggca cgacgtggtg ttccgtcccg gcgtcaacga ggatctggcg
     8281 atgaccgcgg tgcgcggcac gcaggaggtc acgctgttcc ccggcgcgcg cgtcgatggg
     8341 gtctacgcga tgtggtacgg caaggggccg gggctcgatc gcagcatgga cgtgctgaag
     8401 catgccaacg ctgtcggcac ctcgaggcac ggcggcgtgc tcgccgtggt cggcgacgat
     8461 cacgcctgca agtcctcgac gctgccgcac cagtgcgagc acgcgttcat gggcgcttct
     8521 gctcccgttc tcaaccctgc caatgtgcag gagatcctcg acctcggcct gctcggctgg
     8581 gagctgtcgc gttactgcgg ctgctgggtc ggcttcaagg tcatcaccga gaactgcgac
     8641 gcggccgcct cggtggacat cgacccgcac cgcatcagcg tgaagatccc cgaggaattc
     8701 aggctgccgc caggcggcgt gcacgcgcgg ctgcacgaca agccgctggc gcaggaagaa
     8761 cggctcaacc tgtacaagat ctacgcggcg cgcgagttcg cgcgcgtgaa ccacctgaac
     8821 tacgtcgcga tcgacagccc gcgtccgcgc ctgggcatcg tgagcaccgg caaatcctgg
     8881 ctcgacgtgc tgcaggcgct ggaggatctc ggcatcgact gcgaccaggc cgccgcgctc
     8941 ggcatccgcg tgttcaaggt cggtatgcct tggccgctgg atccggtggc gacgcacgcc
     9001 ttcgccgagg ggctcgagga aatcctcgtg gtcgaggaga agcgctcgat catcgaggac
     9061 cagctcaccg ggcagctcta caactggccc gtcgagcggc gtccgcgcgt gatcggggaa
     9121 ttcgacgagc acggccgctc gctgctgccg aacctcgccg agcttacgcc ggcgatggtc
     9181 gcgcgcgcga tcgcgagccg catcgggcgc tattaccagg gcgaggacat gcagcggcgc
     9241 ctggagttcc tcgaccgcaa ggaacgctcg ctcgccacgc cgcgcgcgat catcgagcgc
     9301 acgccgcact actgctcggg ttgcccgcac aacacctcga ccgtggtgcc cgggggcagc
     9361 cgcgccggcg gcggcatcgg ctgccactac atggtcacgt ggatggaccg caacaccgac
     9421 accttcacgc agatgggtgg cgagggcgcg acctggatcg ggcagacccc gttcacggac
     9481 acggcgcacg tgttccagaa cctcggcgac ggcacctatt tccactccgg catcctcgcg
     9541 atccgcgccg cggtcgccgc cggcgtcaac atcacctaca aaatcctcta caacgatgcg
     9601 gtcgcgatga ccggcgggca gccgatcgac ggccagctca cggtggcgca gctcatcgag
     9661 cagctccgcg gcgagggcgt caagcgcatc gcgctggtca gcgacgagcc ggaaaaatac
     9721 ccgcgcgcgc tgcatcacgc cgccggcgtg acgattcatc accgtgacga gctcgatgcc
     9781 gtgcagcggg agtttcgcga aatttgcggc accaccgcga tcgtctacga ccagacctgc
     9841 gcggccgaga agcggcgccg gcgcaagaag ggcgcctatc ccgatcccga gcggcgcgtg
     9901 ttcatcaacg agctggtgtg cgagggctgc ggcgactgcg gcgtgcagtc gaactgcctc
     9961 tcggtgctgc cgaaggaaac cgccttcggg cgcaagcgca tgatcgacca gagcgcctgc
    10021 aacaaggact acagctgcct gaagggcttt tgtccgagct tcgtgaccgt cgagggcggg
    10081 cggctgcgca aaagccgcgc ggtgctggcc gaggtcgcgc tccccacgtt gcccgaaccc
    10141 gtgctgcccg cgctggagcg gccctggaac acggtggtca cgggcatagg cggcaccggc
    10201 gtgctgaccg ttagcgccat cctcggcatg gccgcgcacc tcgagggcaa gggctgcacg
    10261 gtgctgaacc agagcgggct ggcgcagaag ttcggtcccg tcgtgagcca tgtgcgcatc
    10321 ggacgccgcc aggccgacat tcacgcggtg cgcgtggccg ccggcgatgc ggacctgctg
    10381 ctcggctgcg atctcgtggt gagcgcggcc gaggatccgc tggcgaaggt gaacatcgag
    10441 cgctcgcacg cggtcgtgaa cgactt
//
LOCUS       DAORUQ010000112        10342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6806_length_10342_cov_6.101585, whole genome shotgun sequence.
ACCESSION   DAORUQ010000112 DAORUQ010000000
VERSION     DAORUQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_10342_cov_6.101585"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1098
                     /locus_tag="PLW13_10485"
     CDS             <1..>1098
                     /locus_tag="PLW13_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQY70848.1"
                     /translation="PATDGRATRELAPGLLEAASRQPPVSVIPAPAGHAAAGAQPPAR
                     DALARALDRGAAGTHAREATPVRAEAAVAAPGGGLMVAASADALEPARGDADPRGFSV
                     GPAALPSMPAVAPGAADSGESAVQPRAPGTHEKPGNAPGVAMPVAMAVVPPAPRADSA
                     VTGPLAAGAATQRQQAHAPALQAGHPPAPPAPPAPPEVQADARVADNATTSWLQALDE
                     ARELAAGRGLAPQGGDATGASAPFAGERGVAAVPALQALAVATTRAEAGAGPGVFAAP
                     LAVGQAGAQWAGELEARVQWLAGRNLRSAEIRLDPPELGPLQVQVQSHRDGASVHFTT
                     HSATVRDLVEQSLPRLREMLESSGMNLLDVNV"
     assembly_gap    1099..1198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1299..1799
                     /locus_tag="PLW13_10490"
     CDS             1299..1799
                     /locus_tag="PLW13_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HQY70849.1"
                     /translation="MAGEKTADTASGGNNNKKKILLIGAAAALVVLALGATAWFLLGG
                     EAAEEEAQATSAPVAAPAAIYASLGEKFVVTLQEQGRQRYFQASLSALTREESVVKAL
                     ELHAPLIRGRLVALLGEQDFAALRTEAGKLALRDRILATLQDVLQTETGSAGVEQVFF
                     TEFVLQ"
     gene            1835..2797
                     /gene="fliM"
                     /locus_tag="PLW13_10495"
     CDS             1835..2797
                     /gene="fliM"
                     /locus_tag="PLW13_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HQY70850.1"
                     /translation="MQDLLSQDEIDALLHGVGGGEIETAPEVPPPEVQFYDLTSQDRI
                     VRGRMPALERTNERFARQLRSGFRGLLRRGLDVNYEGMQIQKFGEYANSLLAPISINV
                     VRLNPLPGHALVVMDARLVFLLVDNFFGGGRFHAKVEGREFTPSETLVVDRVLETVFA
                     ELEKAWGGSSAVKVQMVQKEASPEAAGIFNANDIAVVSSFSVEFDVSGGKLHLVVPYG
                     MIEPIKELLDRSAVNDANARDSRWESALKENIKDTKVRLNCRVADTEVSLREVIDLEV
                     GDVIPIEAPQPVLGVDDIPLFRVRLGTLKGNLALQVLNNVERPK"
     gene            2925..3182
                     /gene="fliN"
                     /locus_tag="PLW13_10500"
                     /pseudo
     CDS             2925..3182
                     /gene="fliN"
                     /locus_tag="PLW13_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            3340..4836
                     /gene="gltX"
                     /locus_tag="PLW13_10505"
     CDS             3340..4836
                     /gene="gltX"
                     /locus_tag="PLW13_10505"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQY70851.1"
                     /translation="MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGKFILRIEDT
                     DQVRSTRESEAAILESLRWLGLDWDEGPDVGGPHAPYRQSERGEMYREHADYLLEHGH
                     AFRCFCTTERLDALRREQTAAQQQPGYDGHCLTLSQAEVTARLEAGDPWVVRMKVPRE
                     GVCQVQDMLRGQIDFEWTQVDMQVLLKADGMPTYHLANVVDDHLMGITHVLRGEEWIN
                     SAPKHLLLYEYFGWQAPVLCHLPLLRNPDKSKLSKRKNPTSILYYRRMGYLPEALLNY
                     LGRMGWSMPDEREKFTLGEMLCHFDIMRVSLGGPVFDLDKLSWLNALWIREDFTLEQL
                     ADRMIAWAYNRENLLRVLPHLQPRMERLSDFAPAAAYLASGMLPLTEQSFAEVKLERE
                     ALAQLLQFAQWRLEGVAPWEREAIFAAMKTLADQMGLKIRDFLAPLFIAIAGTAASIS
                     VIDSMHMLGSDLSRARIRHALEVLGGISKKKMKDVEKAYAALGAAAGG"
     gene            complement(4856..5650)
                     /locus_tag="PLW13_10510"
     CDS             complement(4856..5650)
                     /locus_tag="PLW13_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70852.1"
                     /translation="MGILELFRLDGKVAVITGAGRGIGAACARAYAEAGASVVIGART
                     RAQIDGVAADIRAAGGKALALPTDVMREEQLEALVEAAIREFGRLDIVVNNAGGTAPG
                     PVLKTSTQDFVNSFRFNVGTAFALSRIAAPRMVESAGGGAIVNISSVAGEHPAAGFAT
                     YGTAKGALSLLTRELAQEFAPKIRVNAIAVGSTRTEALGTVLSNPAIEKKMVELTPMG
                     RLGEVEDIAACALYLASPAASYLTGDIIGVNGGLTTLNLPMPRAFG"
     gene            5798..5873
                     /locus_tag="PLW13_10515"
     tRNA            5798..5873
                     /locus_tag="PLW13_10515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5831..5833,aa:Ala,seq:ggc)
     gene            6083..7882
                     /locus_tag="PLW13_10520"
     CDS             6083..7882
                     /locus_tag="PLW13_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQY70853.1"
                     /translation="MGQYTPPLRDMQFVLHEMLDVETRLRMLPRHADIDIATIDAILE
                     AGGQFCAEVLYPLNAVGDREGCVLHADGSVTTPSGFKEAYRQFAEAGWASLGADPAYG
                     GQGLPHVIDSAFMEMLNSANQAWTMYTGLSHGAYNALQAFGTEEQKALYLPKLVSGDW
                     TGTMCLTEAHAGTDLGMLRTRAEAQADGSYAITGTKIFISAGEHDLSANIVHLVLARM
                     PDAPPGTKGISMFIVPKFLPDADGAPGARNAVRCGALEHKMGIHGNATCVINLDGAKG
                     WMVGQPNKGLNAMFVMMNAARLGVGMQSLGLAEVAYQNSLAYAKERLQGRALTGPKHA
                     DKPADPIIEHPDVRRMLLTQKAYVEAARAFTYWAGLAIDFEHKHPDPQVRQDNADMVS
                     LLTPVVKGFITDNGYESTTLAMQVLGGHGYIVESGLEQHVRDARINMIYEGTNTIQAM
                     DLLGRKVLGDMGAKLMKFGREIDQLVAMHAANPAMQEFTAPLAALWGEVQTVTAEIGQ
                     KALSNPDDAGAAAVPYLRLIGHLVFSFAWCFAVGVALRKLPADGSAGDPFYTAKLATA
                     RFYFARLYPETAALLRQVRAGSAPLMALSPEMF"
     gene            7930..8610
                     /locus_tag="PLW13_10525"
     CDS             7930..8610
                     /locus_tag="PLW13_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY70854.1"
                     /translation="MELYEAMRTTFAAREFTGDPLPDDVLYRILDNARFAPSGGNRQG
                     ARVLVIRAPETRHALAELALPGARRYIAQLRAGENPWTSVVPCGVDPQTIAGTEVPRS
                     MTQPLLDAAVVLVVCIDLRVVASTDMELPRVGVVSGASVYPLVWNILLAARNEGFGGT
                     ITTVAIAEEPAIKALLGIPEHCAVCAVLPLGKPVRQLTRLKRLDVPAFTMLERWDGAP
                     LAKPGSPD"
     gene            complement(8695..9558)
                     /locus_tag="PLW13_10530"
     CDS             complement(8695..9558)
                     /locus_tag="PLW13_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HQY70855.1"
                     /translation="MHLGFSSMNTLFDPPPPVLARYLEERGFESLWYGEHSHIPCAQK
                     TPYPGGGELPTPYKHMMDPYVSLMAAAAATTKLRLGTGIALLMERDVFSQAKTIATLD
                     QLSGGRVIIGTGVGWNREEFGNVSQQPWNKRYGVLRETVAAMRALWRDEEAGYRGEYL
                     NFDPVWLYPKPKQAGGPAIVFGAMGEIGVRHAAQWADGWFPVDIAMPDIPAAIAAFRQ
                     AVRDHGRDPDAVPVTLQAMATPDLDTLKRYRDMGIHRVNVGVAIDMWDKPEQVLPMID
                     RFAPVIPELAG"
     gene            9855..>10342
                     /locus_tag="PLW13_10535"
     CDS             9855..>10342
                     /locus_tag="PLW13_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY70856.1"
                     /translation="METVAQAGSAQAGVDRAALLRALRALLPQASVLARDEELAPYEC
                     DGLSAYRRRPLVVVLPDTIEQVQAVLRLCSARGVPVVARGAGTGLSGGALPLADGVLL
                     SLAKFNRILAVDPLARTARVQPGVRNLAISQAVEHLGLYYAPDPSSQIACTIGGNVAE
                     NSG"
BASE COUNT         1594 a         3482 c         3619 g         1547 t
ORIGIN      
        1 cccggcgacg gatggcaggg cgacccggga gctggcgccg gggctgctcg aggcagcatc
       61 gcgccagccc ccggtcagcg tgataccggc accggccggg cacgcggccg ctggcgcgca
      121 gcccccggca cgggacgcac tggcgcgtgc gctggatcga ggcgccgctg gcacgcacgc
      181 gcgcgaagcg accccggtgc gcgccgaggc tgcagtggcg gcgccgggcg gtggcctcat
      241 ggttgcggcg agcgccgatg ccctcgaacc ggcgcgcggc gatgcggacc cgcgcgggtt
      301 ttcggtcggg cccgcggcgc taccctccat gcccgccgtg gcgccgggcg cggcggatag
      361 cggcgagtcc gcggtgcaac cgcgcgcccc cgggacgcat gaaaagcccg ggaacgcccc
      421 gggcgtggcg atgccggttg ccatggccgt ggtgccaccg gctccccgcg cggacagtgc
      481 agtgacgggg ccgcttgccg ctggcgccgc gacgcagcgc cagcaggcgc acgcgccagc
      541 gttgcaggcc gggcatcccc ctgcgccgcc ggcgccgccg gcgccgccag aagtgcaggc
      601 ggatgcccgc gttgccgaca acgccacgac atcgtggctg caggcgctcg acgaggcgcg
      661 ggaactcgcc gccgggcgcg gattggcgcc gcagggcggc gatgccacgg gcgcatcggc
      721 accgtttgcc ggcgagcgtg gcgttgccgc ggtgccggcg ctgcaggcgc ttgcggtcgc
      781 aacgacgcgc gccgaggcgg gcgccggacc gggcgtgttc gcggccccgc tggcggtcgg
      841 gcaggccggt gcgcagtggg ccggcgagct ggaagcccgt gtgcagtggc tggccgggcg
      901 taacctgcgt tccgccgaga tccggctcga tccgcccgag ctgggcccgc tgcaggtgca
      961 ggtgcagtcc catcgcgacg gcgccagcgt gcacttcacc acccacagcg cgacggtgcg
     1021 cgacctggtc gagcagtcgc tgccgcgcct gcgcgagatg ctggagagca gcggcatgaa
     1081 cctgctcgac gtcaatgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     1201 gtgtgctaag tttcgcgccc tgacaggggc agggcgtcct tgctggcatg ttgattgctc
     1261 gacatgaggc aagggcacca cagtgacgga actttggcat ggcgggtgag aagacggcgg
     1321 acaccgcgtc cggcgggaac aacaacaaga aaaagatcct gctgatcggc gccgcggcgg
     1381 cgctagtggt gctggcgctt ggcgcgacag cgtggttcct gctcggcggc gaggctgccg
     1441 aggaggaagc gcaggcaacg agcgcaccgg ttgcggcccc cgcggcgatc tacgcttcgc
     1501 tcggcgagaa gttcgtggtg acgctgcagg agcaggggcg tcagcgctac ttccaggcca
     1561 gcctgagcgc gctgacgcgc gaggagtcgg tggtcaaggc gctggaactg cacgcgcccc
     1621 tgatccgcgg acgcctggtg gcgctgctcg gcgagcagga cttcgcggcg ctgcgcaccg
     1681 aggcgggcaa gctcgcgctg cgcgatcgca tcctcgcgac gctgcaggac gtgctgcaga
     1741 ccgagaccgg ttccgccggc gtcgagcagg tcttctttac cgaattcgtc cttcaatgac
     1801 ccgtcgcgcg gtcgcttgag agcggagagc cagcgtgcag gacttgctgt cacaggacga
     1861 gatcgatgcg ctgctccacg gcgtgggcgg cggcgagatc gaaaccgcgc ccgaggtgcc
     1921 gccgcccgag gtccagttct acgacctcac cagccaggac cgtatcgtgc gcgggcgcat
     1981 gccggcgctg gagcgcacca acgagcgttt cgcgcggcag ctgcgcagcg gttttcgcgg
     2041 gctgctgcgc cgcggcctcg acgtgaacta cgagggcatg cagatccaga agttcggcga
     2101 gtacgccaac agcctgctgg cgcccatcag catcaacgtg gtgcggctga atccgctgcc
     2161 cggacacgca ctggtggtga tggacgcgcg gctcgtgttc ctgctggtcg acaatttctt
     2221 cggcggcggg cgcttccacg ccaaggtcga ggggcgcgag ttcaccccct ccgagacgct
     2281 ggtggtggac cgcgtgctcg agaccgtctt cgcggagctc gagaaggcct ggggcggcag
     2341 ctccgcggtg aaggtgcaga tggtgcagaa ggaggcgagc ccggaggccg ccgggatctt
     2401 caacgccaac gacattgcgg tggtcagctc cttcagcgtc gagttcgacg tgagcggcgg
     2461 caagctgcac ctcgtggtgc cctacggcat gatcgagccg atcaaggagc tgctcgaccg
     2521 ctccgcggtg aacgacgcga acgcgcgcga cagccgctgg gagtcggcgc tgaaggaaaa
     2581 catcaaggac acgaaagtca ggctgaactg ccgcgtcgcg gataccgagg tgtcgctgcg
     2641 cgaggtgatc gacctggagg tcggcgacgt gatccccatc gaagcgccgc aacccgtgct
     2701 gggcgtcgac gacatcccgc tgttccgggt acgcctgggc acgctgaagg gcaacctggc
     2761 gctgcaggtg ctgaataacg tcgagcgacc gaaatgagag ccgaatccga ggaagtgctg
     2821 caatgagcaa tgaagacacc ctgccgggcc agggcgggcg cgccacggtg caggccgatg
     2881 cgatgcccga cttcggcggc gtcgccccga cgcgtgccct cagccccgac ctcgagatga
     2941 tcctcgacat cccggtccgg ctctcgatgg aagtgggcaa cacgcagatc tccatccgcg
     3001 acctgctgca actggggcag ggctcggtga tcgagctgga tcgtctcgcg ggcgaaccgc
     3061 tcgacgtgct ggtcaacggc acgctgatcg cccacggcga ggtggtcgtc gtgaacgaca
     3121 agttcggcat ccgcatgacc gatgtgatca gcccttcgga gcggatcaag aagctcggct
     3181 gaggcggcgc gcacagaacc tgccgagcgg gccttgccgg ccggcgacgc tccctgcgcg
     3241 accgtcgccg gtgtcacgcc tgctggctat actagcgccc ccatttcgac cgctcgtgct
     3301 gccttgctct gcgcgggcct tgccaggatt cgtttccaga tgaccgttcg tacccgcatc
     3361 gccccgtcgc ccaccggcga cccgcacgtc ggcaccgcct acatcgccct gttcaacctc
     3421 tgcttcgcgc gccagcacgg gggcaagttc atcctgcgca tcgaggacac cgaccaggtg
     3481 cgcagcacgc gcgaatccga ggccgcgatc ctcgaatcgc tgcgctggct cgggctcgac
     3541 tgggacgagg gtcccgacgt cggcggcccg cacgccccgt accgccagag cgagcgcggc
     3601 gagatgtacc gcgagcacgc ggattacctg ctggagcacg ggcacgcgtt ccgctgcttc
     3661 tgcaccaccg agcgactgga tgccctgcgc cgcgagcaga ccgccgccca gcagcagccg
     3721 ggctacgacg gccactgcct gacgctcagc caggccgagg tgacggcgcg gctcgaggcg
     3781 ggcgacccct gggtcgtgcg catgaaggtg ccgcgcgagg gcgtgtgcca ggtgcaggac
     3841 atgctgcgcg ggcagatcga cttcgagtgg acgcaggtcg acatgcaggt gctgctgaag
     3901 gccgacggca tgccgaccta ccacctcgcc aacgtggtcg atgaccacct gatgggcatc
     3961 acgcacgtgc tgcgcggcga ggagtggatc aactcggcgc ccaagcacct gctgctctac
     4021 gaatacttcg gctggcaggc gccggtgctg tgccacctgc cgctgctgcg caaccccgac
     4081 aagagcaagc tcagcaagcg caagaacccg accagcatcc tgtactaccg gcgcatgggt
     4141 tacctgcccg aggcgctgct caactacctc ggccgcatgg gctggtcgat gcccgacgag
     4201 cgcgagaagt tcacgctggg cgagatgctg tgccatttcg acatcatgcg cgtctcgctg
     4261 ggcgggccgg tgttcgacct cgacaagctg agctggctga acgcgctgtg gatccgcgag
     4321 gacttcacgc tcgagcaact cgccgaccgc atgatcgcct gggcctacaa ccgcgagaac
     4381 ctgctgcgcg tgctgccgca cctgcaaccg cgcatggagc gcctgagcga tttcgcgccc
     4441 gccgcggcct acctcgcctc gggcatgttg ccgctcacgg aacagagctt cgccgaggtg
     4501 aagctcgagc gcgaggcgct ggcgcagttg ctgcagttcg cgcaatggcg cctcgagggc
     4561 gtggcgccct gggagcgcga ggcgatcttc gcggcgatga agacgctggc cgaccagatg
     4621 ggcctgaaga tccgcgattt cctcgcaccg ctgttcatcg cgatcgcggg caccgcggcc
     4681 tcgatctcgg tgatcgactc gatgcacatg ctgggctcgg atctttcgcg cgcgcgcatc
     4741 cgccacgcgc tggaggtgct cggcggcatc tcgaagaaaa aaatgaagga cgtcgagaaa
     4801 gcctacgccg cgctgggcgc ggcggcgggc ggctgacgcg aagcgccgcg cgggctcagc
     4861 cgaaggcgcg cggcatcggc aggttcagcg tggtcagccc gccgttcacg ccgatgatgt
     4921 cgccggtcag gtaggacgcg gcgggggagg ccaggtacag cgcgcacgcg gcgatgtcct
     4981 cgacctcgcc gaggcggccc atcggcgtaa gttccaccat tttcttctcg atggccgggt
     5041 tcgacagcac cgtgcccagg gcctcggtgc gcgtcgagcc gaccgcgatc gcgttcacgc
     5101 ggatcttcgg cgcgaattcc tgcgccagct cacgcgtcag cagcgacagc gcgcccttgg
     5161 cggtgccgta ggtcgcgaat ccggccgccg ggtgctcgcc ggcgaccgag gaaatgttca
     5221 cgatggcgcc gccgccggcg ctctcgacca tgcgcggcgc ggcgatgcgc gagagcgcga
     5281 acgcggtgcc cacgttgaag cgaaaggaat tcacgaagtc ctgcgtggag gtcttcagta
     5341 ccggtcccgg cgccgtgccc ccggcgttgt tcaccacgat gtcgagacgg ccgaactcgc
     5401 ggatcgcggc ctcgaccagc gcttcgagct gctcctcgcg catcacgtcc gtcggcagtg
     5461 ccagcgcctt gccacccgcg gcgcggatgt ccgcggcgac gccgtcgatc tgcgcgcggg
     5521 tgcgcgcgcc gatcaccacg ctggcgccgg cctcggcata ggccctggcg caggccgcgc
     5581 cgatgccgcg cccggcgccg gtgatcacgg cgaccttgcc gtcgaggcgg aacagttcga
     5641 ggatgcccat gggtttttgc ctgttatgtg acgggaaggg cgcaggctag caagcgcctg
     5701 cgcggccgcc aagagcctcg tgatttgcat tcgcggggtg ccgattttca cgcttgacag
     5761 tcctctggcg gctccctacc atgcgcgctc ctttcgaggg ggcatagctc agctgggaga
     5821 gcgcttgcat ggcatgcaag aggtcggcgg ttcgatcccg cctgtctcca ccaaacaccg
     5881 agaaagcgcc gcatgtcggc gcttttttcg ttctggcgcc caggactccc tgcgggatcc
     5941 ccgcgtgtct acaccaaagt ccgagaaggc gccgcatgct cgacacgcgg tggacgctgg
     6001 cacgggactg ctgatccggc gggctggatt tggagtaacc ttgtcccaga cgtgccaacc
     6061 ccgttaatgc cggagacctt ccatgggcca gtacaccccg ccgcttcgcg acatgcagtt
     6121 cgtgctgcac gagatgctcg acgtcgagac ccggctgcgg atgctcccgc ggcacgccga
     6181 catcgacatc gccaccatcg acgcgatcct cgaggccggc ggccagttct gcgccgaggt
     6241 gctgtacccg ctgaacgcag tgggcgaccg cgagggctgc gtgctgcatg ccgacggcag
     6301 cgtcaccacg ccgagcgggt tcaaggaagc ctaccgccag ttcgccgagg ccggctgggc
     6361 gtcgctcggg gcggatccgg cctacggcgg tcagggcctg ccgcacgtca tcgacagtgc
     6421 cttcatggaa atgctgaact ccgccaacca ggcctggacc atgtacacgg gcctgtctca
     6481 cggggcctac aacgcgctgc aggccttcgg caccgaggag cagaaggcgc tgtacctgcc
     6541 gaaactcgtc tcgggcgact ggaccggcac gatgtgcctg accgaagcgc acgccggcac
     6601 cgacctcggc atgctgcgca cgcgcgccga agcgcaggcc gacggcagct acgcgatcac
     6661 cggcaccaag atcttcattt cggcgggcga acacgacctg tcggcgaaca tcgtgcacct
     6721 cgtgctcgcg cgcatgcccg acgcgccgcc cggcaccaag ggcatctcga tgttcatcgt
     6781 gccgaagttc ctgccggatg ccgacggcgc gcccggcgcg cgcaacgcgg tgcgctgcgg
     6841 cgcgctcgag cacaagatgg gaatccacgg caacgcgacc tgcgtgatca acctcgacgg
     6901 tgcgaagggc tggatggtgg gtcagcccaa caagggcctg aatgcgatgt tcgtgatgat
     6961 gaacgccgcg cgactcggcg tcggcatgca gtcgctcggg ctggccgagg tcgcctacca
     7021 gaactcgctc gcctacgcga aggagcggct gcagggacgc gcgctgaccg gcccgaagca
     7081 cgccgacaag ccggccgacc cgatcatcga gcacccggac gtgcggcgca tgctgctcac
     7141 gcagaaggcc tacgtcgagg ccgcgcgcgc cttcacctac tgggccgggc tcgcgatcga
     7201 tttcgagcac aagcaccccg acccgcaagt gcgccaggac aacgcggaca tggtgtcgct
     7261 gctgaccccg gtggtgaagg gcttcatcac cgacaacggc tacgagagca ccacgctcgc
     7321 gatgcaggtg ctgggcggcc acggctacat cgtcgaatcg gggctagagc agcacgtgcg
     7381 cgatgcgcgc atcaacatga tctacgaggg caccaacacg atccaggcca tggacctgct
     7441 gggccgcaag gtgctcggcg acatgggcgc gaaactgatg aagttcggcc gcgagatcga
     7501 tcagctcgtc gccatgcatg ccgcgaaccc ggcaatgcag gaattcacgg ccccgctcgc
     7561 cgcgctgtgg ggcgaggtgc agaccgtcac ggccgagatc ggccagaagg cgctctccaa
     7621 tcccgacgat gccggcgccg ccgcggtgcc ctacctgcgc cttatcgggc acctggtgtt
     7681 ctccttcgcg tggtgtttcg cggtgggtgt cgcgctgcgc aagctcccgg ccgatggcag
     7741 cgcgggcgac ccgttctaca ccgcaaagct ggcgacggcg cgcttctact tcgcgcggct
     7801 ctacccggag actgcggcgc tgctgcgcca ggtgcgtgcc ggctcggcgc cgctgatggc
     7861 gctcagcccg gagatgttct gagagtcgcc gccccgctga cggctccagg cggtccgctg
     7921 tcgtctgaca tggaactgta cgaggcgatg cgcaccacct tcgcggcgcg ggaattcacc
     7981 ggcgacccgc tgccggatga cgtcctctac cgcatcctgg acaatgcgcg cttcgcgccg
     8041 agcggcggca atcgccaagg ggcgcgggtg ctggtgatcc gtgctccgga gacgcgccac
     8101 gcgctggccg agctcgcgct gccgggtgcc aggcgctaca tcgcccagtt gcgggcgggc
     8161 gagaatccgt ggacaagcgt ggtgccctgc ggcgtggatc cacagaccat cgcgggcacc
     8221 gaggttccgc gctcgatgac gcagccgctg ctcgacgcgg cggtggtgct ggtggtctgc
     8281 atcgacctgc gcgtggtcgc ctccaccgac atggaactgc cccgcgtggg cgtggtgagc
     8341 ggcgcctcgg tctatcccct ggtctggaac atcctgctgg ccgcgcgcaa cgaaggcttc
     8401 ggcggcacca ttacgaccgt cgccatcgcc gaggagccgg ctatcaaggc cctgctcggc
     8461 ataccggagc actgcgcggt gtgcgccgtg ctgccgctcg gcaagccggt caggcagctg
     8521 acgcgcctga aacggctgga cgtgcccgcg ttcacgatgc tggagcgctg ggacggcgcg
     8581 ccgctggcta agcccgggtc gccggactga gtacaaagcc ctgcgccacg gcggggagca
     8641 gggcgtttgc cgatgtcatg cccgcgatac agggcaacgc gtacagttgg agcctcagcc
     8701 ggccagttca gggatcaccg gcgcgaaccg gtcgatcatc ggcagcacct gctcgggctt
     8761 gtcccacatg tcgatggcca cgccgacgtt cacgcggtgt atacccatgt cgcggtagcg
     8821 cttcagggtg tcgaggtcgg gtgtggccat cgcctgcagc gtgacgggca ccgcatcggg
     8881 atcacggccg tggtcgcgca cggcctggcg gaaggcggca attgccgcgg ggatatcggg
     8941 catcgcgata tcgaccggga accagccgtc ggcccactgc gcggcatgcc tgacgccgat
     9001 ctcgcccatg gcgccgaaga cgatcgcggg gcctccggcc tgtttgggct tggggtagag
     9061 ccagaccgga tcgaaattga ggtactcgcc gcggtagccg gcttcctcgt cgcgccacag
     9121 cgcgcgcatc gccgcgacgg tctcgcgcag cacgccgtag cgcttgttcc acggctgctg
     9181 gctcacgttg ccgaactctt cccggttcca gcccacgccg gtgccgatga tcacgcgccc
     9241 gcccgagagc tggtccagcg tggcgatggt cttggcctgc gagaacacgt cgcgctccat
     9301 cagcagggcg atgccggtgc ccaggcgcag cttcgtggtg gccgccgccg ccgccatcag
     9361 cgagacatag ggatccatca tgtgcttgta gggcgtcggc aattcgccgc cacccgggta
     9421 gggcgtcttc tgcgcacagg ggatatggct gtgctcgccg taccacagcg actcgaagcc
     9481 gcgctcctcg aggtagcgcg cgagcaccgg cggtggcggg tcgaacaggg tgttcatcga
     9541 actgaagccc aggtgcatgg atctctccgt gttgcccgcg cgcgggcgcg gtcttgttgt
     9601 gaaacggcgt ggcgcggctc gtgcgcaccg cggtaacgga taataccatc aataaaaaat
     9661 gtttttcgat gggtggcggc gcaacgctgc gcagccgggg ccgattatgc gccgcccggt
     9721 ggcaggcgca tcgctcgttc gggtgaagcg ccgcagccgc ccgggcctgc gtccctgcgc
     9781 caggccgcgg ccaacacggc tgtggcggca tttgctagca tgcggcggga tcggacgaac
     9841 ggacgcaagg atcaatggag actgtcgccc aggctggcag cgcgcaggca ggcgtggatc
     9901 gcgcggcctt gctgcgcgcg ctccgggctt tgctgccgca ggcctcggtg ctggcgcgtg
     9961 acgaggagct ggcgccctac gagtgcgatg gcctgtccgc ctaccgccgc cgcccgctcg
    10021 tggtcgtgtt gcccgatacc atcgagcagg tgcaggcggt gctgcggctg tgcagcgcgc
    10081 gcggcgtgcc ggtggtcgcg cgcggggcgg gcacggggct ctcgggcgga gcgctgccgc
    10141 tcgccgacgg tgtgctgctc agcctggcga aattcaaccg tatcctggct gtcgatccgc
    10201 tggcgcgcac cgcgcgcgtg cagccgggcg tgcgcaacct cgcgatctcg caggcggtgg
    10261 agcacctggg gctgtactac gcgcccgatc cctcgtcgca gatcgcctgc accatcggcg
    10321 gcaacgtggc cgagaattcc gg
//
LOCUS       DAORUQ010000113        10341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6808_length_10341_cov_6.292242, whole genome shotgun sequence.
ACCESSION   DAORUQ010000113 DAORUQ010000000
VERSION     DAORUQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_10341_cov_6.292242"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="mazG"
                     /locus_tag="PLW13_10540"
     CDS             complement(<1..815)
                     /gene="mazG"
                     /locus_tag="PLW13_10540"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016715459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQY70857.1"
                     /translation="MRRHDIGDLRELMRRLRDPQTGCPWDLKQDHASLLEHTLEEVYE
                     LADAIERGDSAQMRDELGDYLFQAVFYAQIAAENDEFDFDDVTDGIVRKLLRRHPHVF
                     PDGTLASRRDPGSAPQTREIKATWERLKEEERRTRALGAILDDVPVALPALQRAEKLQ
                     RRAASAGFDWTSWDGVVVKLEEELEEVREAARGDDAAAREDELGDLLFCCVNLARHLG
                     VNAENALRRANRKFETRFRAVEEEIRRRGLAIGDCELALLDSIWDEVKARSRR"
     gene            complement(830..3055)
                     /locus_tag="PLW13_10545"
     CDS             complement(830..3055)
                     /locus_tag="PLW13_10545"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQY70858.1"
                     /translation="MVKIRDAHSLEGPLPDDGASLLARVPAAQAVNGAQRVAAALDAV
                     LAAYPAEEDAAHRRERIGAALDILETLATLGLDADALCAGVLCVPVAEGRIGAEALAD
                     GFGAGVAALIAGVQRMDALGLDPHEHTRRHSFANRQRQGENLRRMLVSMIDDPRVALV
                     KLAERVQALRALGTGNDSSGAVQTARAAMDIYAPLAHRLGVGQIKWELEDLAFRHLQP
                     GDYRGIAKLLDERRADREQFIAEATARLREALAAAGVKAELSGRPKHLYSIWRKMQRK
                     GIGITEVYDVRALRVLVGSVADCYSTLGIVHGLWRNLPGEFDDYIANPKPNGYRSLHT
                     AVIGDGGRVLEVQIRTGDMHQEAELGICAHWLYKSGEPVPAGRDYEDKIGWLRQVLGW
                     GGELDAGELISEHLRREVGAERVYVLTPDGHVVDLPAGATPVDFAYQVHSELGHRCRG
                     ARVDGRIVSLDYTLSTGERVEIIRGKNAAPNRDWLRAGNEFVHTARARSKIRHWFREQ
                     DREGLIAAGRALLERELRRVALTPPQLPALARGLGFASADDLLASLGSGETGAGQVLG
                     FLAPEEQGLPATVPEPARAAARKRVRAAVAVEGIDNLMTNFAGCCKPLPGEAIAGFIT
                     GGRGVSVHRRDCSKLQRLAQDNPARIIDVHWQDAQPRTHAVDLRVVAHDRAGLLKDLV
                     TLLGNERVNVLDLGSTVDRARQLATVRLTIEIASLEALGRILEKLGRVGGVIAVQRHS
                     E"
     gene            complement(3065..4012)
                     /gene="cysM"
                     /locus_tag="PLW13_10550"
     CDS             complement(3065..4012)
                     /gene="cysM"
                     /locus_tag="PLW13_10550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HQY70859.1"
                     /translation="MYRHQNDPAASAAAAPAHVEFPTVEASVGETPLVRLQRMQAGVN
                     NTILLKLEGNNPAGSVKDRPALSMIRRAEERGEIRPGDTLIEATSGNTGIALAMAAAI
                     RGYRMVLIMPAHMSEERRQVMRAFGAELIDVPREGGMEAARDLALRMQSEGRGRVLDQ
                     FANPDNPLAHYEATGPEIWRQSHGRITHFVSSMGTTGTIMGVSRYLKEMNPAIRIVGL
                     QPADGSSIPGIRRWPHEYLPRIFERERVDDVLDIAQHEAESTMRALASREGILCGVSS
                     GGAVAGALRVAAHARDAVIVAIVCDRGDRYLSTGVFDAR"
     gene            complement(4105..4821)
                     /gene="recO"
                     /locus_tag="PLW13_10555"
     CDS             complement(4105..4821)
                     /gene="recO"
                     /locus_tag="PLW13_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQY70860.1"
                     /translation="MDRQPAFVLHRRAWRETSLLLDLLTRDFGRIGVIARGARGGRRG
                     SGASCQPFQPLLVSWSGRADLKTLSAAEAVAPAPAIAGERLYTALYVNELLIRLLMQH
                     DAHSALFDAYAALLPGIASAEDLEPLLRRFELLLLRELGYGMEFVHDSSTGQALEPAG
                     QYLLSHDGGFHAAPASADPRAYPGRVLAQIGREDFSGPETRRYAKRLMREALAQLLGA
                     RPLHSRGYFRARHAPPADAI"
     gene            complement(4862..5758)
                     /gene="era"
                     /locus_tag="PLW13_10560"
     CDS             complement(4862..5758)
                     /gene="era"
                     /locus_tag="PLW13_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQY70861.1"
                     /translation="MSTERCGMVAIVGRPNVGKSTLLNHLLGQKISITSRKPQTTRHR
                     ILGVSTHGAAQIVYVDTPGLHQGEGKAINRAMNRAALSAVRDVDLVLMLVDRLQWLEP
                     DEQVLAAVRTAGKPVLLIINKTDQIEQKDALLPHIAALQAKYPFAAIVPVSALKGHNL
                     EALEREIQTLLPEAPHLFPPDQITDRSERFMVAEIVREKLMRQLGEELPYANAVQIEE
                     FRQEGKTIHISALILVEKAGQKAIVIGRAGARLKLIGAEARIDMERLLGTKVMLKLWV
                     KVKSGWSDDERALKSLGYQEES"
     gene            complement(5761..6456)
                     /gene="rnc"
                     /locus_tag="PLW13_10565"
     CDS             complement(5761..6456)
                     /gene="rnc"
                     /locus_tag="PLW13_10565"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQY70862.1"
                     /translation="MPRDAGPDIARLQSRIGHEFARRELLELALTHASFGAGNNERLE
                     FLGDAVLNLGIAAELYARYPALREGQLHRLRVSLVRGETLADIGRELGLGAVLRLGAG
                     EASSGGRERDSILADAVEALLGAICLDAGTDAALGVVRRWYAARLAGLEPGISHKDPK
                     TELQEYLQARRAPLPRYELAATSGAPGQQHFRVLCHVRGLAVPVEAQAGTKRAAEQRA
                     ARTALARLQGAEQ"
     gene            complement(6446..6859)
                     /locus_tag="PLW13_10570"
     CDS             complement(6446..6859)
                     /locus_tag="PLW13_10570"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HQY70863.1"
                     /translation="MNSSSRSRQAGFTIWQWMVIVLVGGFLLTVGFSLAPLYINNYTV
                     RATVQALQNEPELGRKSTQEIRLAVERKFDVNQIEAIQAVCRDKARPCLKVEKTKTHL
                     IIDANYEARTHVMGNVDAIVNFDKNRVEIVIPGAS"
     gene            complement(6910..7749)
                     /gene="lepB"
                     /locus_tag="PLW13_10575"
     CDS             complement(6910..7749)
                     /gene="lepB"
                     /locus_tag="PLW13_10575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQY70864.1"
                     /translation="MDIDFPLILVALTFVALVIWALDVLWLRPARRRRDAALRARFPR
                     WSEAGSADALGYAAEAGKALKEPVPVEYARSFLPVLLIVLVLRSFLVEPFQIPSSSMV
                     PTLLVGDYILVNKFAYGIRLPVLNTKVLDVGTPQRGDVMVFFPPNDKRYFIKRVIGLP
                     GDRVAYRDKVLYVNGEQMPQSLIAQLPPLHPSYQILEEELAGVPHVVRKELMRRIPDD
                     FEHTVSPGHYFMMGDNRDNSSDSRVWGEVPERNIVGKAFAIWMHWGSFFTMPSFDRVG
                     TIR"
     gene            complement(7761..9563)
                     /gene="lepA"
                     /locus_tag="PLW13_10580"
     CDS             complement(7761..9563)
                     /gene="lepA"
                     /locus_tag="PLW13_10580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQY70865.1"
                     /translation="MTALNRIRNFSIIAHIDHGKSTLADRFIQVCGGLSGREMQEQVL
                     DSMDIERERGITIKAQSVTLAYKARDGETYQLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAGQGVEAQSVANCYTAIEQGLEVLPVLNKMDLPQAEPERVAGEIEDIIGIDASH
                     ACRVSAKSGLGIDELLEELVRVIPPPEGDPDAPLQALIIDSWFDTYLGVVSLVRVMNG
                     TLRVRDRIVTKSIGRAHEVDQVGVFTPKRLQRQSLSAGEVGFIVAGIKDIHGAPVGDT
                     ITHLKTADSAPALPGFQKVKPQVYAGLFTVNSEDYEDFRDALAKLTLNDASLFYEPET
                     SDALGFGFRCGFLGTLHMEIIQERLEREYDLDLITTAPTVIYEVLTARGETIMVDNPS
                     RLPDPGMVEEMREPIVRANILVPQDYVGAAINLCVEKRGVQKDMQFLGRQVSLTWELP
                     LSEVVLDFFDRLKSLTRGYGSLDYSFERFQAANLVRLDVLINGDRVDALAIIVHRDQA
                     ASRGRVLVEKMRELIPRQMFDVAIQAAIGGHIIARQTVKALRKNVIAKCYGGDVTRKK
                     KLLEKQKEGKKRMKQVGRVEIPQAAFLAVLKVDS"
     gene            complement(9726..>10341)
                     /locus_tag="PLW13_10585"
     CDS             complement(9726..>10341)
                     /locus_tag="PLW13_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQY70866.1"
                     /translation="RGWLGVGIQDVDRALAESFGLDKPAGALISQVEPGGPAEKAGVR
                     VGDVVIEFDGQPVNESADLPHIVGMVKPGTRSAMKVIREGKKTDLRITVGELEGAQET
                     GAVAQASATGGRIGLVIDDLDQQQKAQLRINGGVVVREVARGKAGDRAGLRPGDVITL
                     IGSESVRDARRFSEIVAALPSGTHVPVRLLRGGQAGFVAIRIED"
BASE COUNT         1494 a         3661 c         3510 g         1676 t
ORIGIN      
        1 ctgcggcgcg aacgcgcctt gacctcgtcc cagatgctgt cgagcagcgc gagctcgcaa
       61 tcgccgatcg cgaggccgcg acgccggatc tcctcctcga cggcgcgaaa gcgcgtctcg
      121 aacttgcggt tggcgcgacg cagcgcgttc tcggcgttga cgccgaggtg acgcgcgagg
      181 ttgacgcagc agaacagcag gtccccgagc tcgtcctcgc gcgccgcggc atcgtcgccg
      241 cgcgcggcct cgcgcacctc ctccagttcc tcctcgagct tcacgaccac gccgtcccag
      301 ctcgtccagt cgaaaccggc ggacgcggcg cggcgctgca gtttctcggc gcgctgcagc
      361 gccggcagcg ccaccggcac gtcgtcgagg atggcaccga gggcgcgagt gcggcgctcc
      421 tcctccttca gccgttccca ggtggccttg atctcgcgcg tctgcggcgc gctgccggga
      481 tcgcggcggc tcgcgagcgt gccgtcggga aacacgtgcg gatggcgacg cagcagcttg
      541 cgcacgatgc cgtcggtgac gtcgtcgaag tcgaactcgt cgttttccgc cgcgatctgg
      601 gcgtagaaca cggcctggaa caggtagtcg ccgagttcgt cgcgcatctg tgcggaatca
      661 ccgcgctcga tggcgtcggc gagctcgtag acctcctcca gcgtgtgctc gagcagcgag
      721 gcgtgatcct gtttcaggtc ccaggggcag cccgtctgcg ggtcacgcag ccggcgcatg
      781 agttcgcgca gatcgccgat gtcgtgacgc cgcatgccgg tctcccctgt cattccgaat
      841 gacgctgcac cgcgatcacg ccgccgacgc gcccgagctt ctcgaggatg cgccccagcg
      901 cctcgagcga ggcgatctcg atcgtgagcc gcacggtcgc aagctgccgc gcacggtcca
      961 ccgtgctccc aaggtcgagc acgttgaccc gttcgttgcc gagcagcgtg accagatcct
     1021 tcagcagccc cgcgcggtcg tgcgcgacga cccgcaggtc gacggcgtgc gtgcgcggct
     1081 gcgcgtcctg ccagtgcaca tcgatgatgc gcgcgggatt gtcctgcgcc agccgctgca
     1141 gcttggagca gtcgcgccgg tgcacgctga cgccgcgccc tccggtgatg aagcccgcta
     1201 tcgcctcgcc cggcaagggc ttgcagcagc ccgcgaagtt cgtcatcagg ttgtcgatgc
     1261 cctcgaccgc caccgcggcg cgcacccgct tgcgcgccgc ggcgcgcgcg ggctccggca
     1321 cggtcgccgg caggccctgt tcttccggcg cgaggaagcc cagcacctgc ccggcgccgg
     1381 tctcaccgct gccgagcgag gcgagcagat cgtcggccga cgcgaatccc aggccgcgcg
     1441 cgagcgccgg caactgcggc ggcgtcagcg ccacgcggcg cagttcgcgc tcgagcagcg
     1501 cgcgcccggc cgcgatcagg ccctcgcgat cctgctcgcg gaaccagtgg cggatcttgc
     1561 tgcgcgcgcg cgcggtgtgc acgaactcgt tgccggcacg cagccagtcg cggttcggcg
     1621 ccgcgttctt gccgcggatg atttcgacgc gctcgccggt cgacagcgtg tagtcgaggc
     1681 tgacgatacg cccgtcgacg cgcgcgccgc ggcagcggtg accgagctcg ctgtgcacct
     1741 ggtaggcgaa atcgaccggt gttgcgcccg ccggcaggtc caccacgtgc ccgtccggcg
     1801 tcagcacgta gacgcgctcg gcaccgactt cgcggcgcag gtgctcggag atgagctcgc
     1861 ccgcatcgag ctcgccgccc cagcccagca cctggcgcag ccagccgatc ttgtcctcgt
     1921 agtcgcggcc cgcgggcacg ggctcgccgc tcttgtagag ccagtgcgcg cagatgccga
     1981 gctcggcctc ctggtgcatg tcgccggtgc ggatctgcac ctccagcacc ctgccgccgt
     2041 cgccgatgac cgcggtatgc agcgagcgat agccgttggg cttggggttg gcgatgtagt
     2101 cgtcgaactc gccggggagg ttgcgccaca gtccgtgcac gatcccgagc gtcgagtagc
     2161 agtcggccac cgaaccgacc agcacgcgca gcgcgcgaac gtcgtagacc tcggtgatgc
     2221 cgatgccctt gcgctgcatc ttgcgccaga tgctgtagag gtgcttgggg cgaccggaga
     2281 gctcggcctt cacgcccgcc gccgcgagcg cctcgcgcag ccgcgcggtc gcctcggcga
     2341 tgaactgctc gcggtcggcg cgccgctcgt cgagaagctt cgcgatgccg cgataatcgc
     2401 cgggctgcag atggcggaac gcgaggtcct cgagttccca cttgatctgg cccacgccga
     2461 ggcggtgtgc cagcggcgca tagatgtcca tcgcggcgcg cgccgtctgc acggcgccgg
     2521 acgagtcgtt gccggtgccg agcgcgcgca gcgcctgcac gcgctcggcg agtttcacca
     2581 gcgcgacgcg cggatcgtcg atcatcgaca ccagcatgcg gcgcaggttc tcgccctggc
     2641 gctggcggtt ggcgaagctg tggcgccgcg tgtgctcgtg cggatcgagt ccgagcgcat
     2701 ccatgcgctg cacgccggcg atcagcgccg ccacacccgc gccgaagcca tcggccagcg
     2761 cctcggcgcc gatgcgcccc tcggcgaccg gcacgcacag cacgccggca cagagcgcgt
     2821 cggcgtccag gccgagcgtc gccagcgttt cgaggatgtc cagcgccgcg ccgatgcgct
     2881 cgcgccggtg cgcggcgtct tcctccgcgg ggtaggctgc gagcacggca tcgagggcgg
     2941 cggcaacacg ctgcgccccg ttcaccgcct gcgcggcagg cacgcgcgcg agcaggctcg
     3001 cgccgtcatc gggcagcggc ccctcgagcg agtgggcgtc gcggatcttg accatggtcg
     3061 ggactcagcg cgcgtcgaac acgccggtgg acaggtagcg atcgccgcgg tcgcagacga
     3121 tcgcgacgat caccgcatcg cgcgcgtgcg ccgccacgcg cagcgcgccg gcgaccgcgc
     3181 cgcccgagga cacgccacag agtatgcctt cgcggctcgc gagcgcgcgc atcgtgctct
     3241 cggcctcgtg ctgcgcgatg tcgagcacgt catcgacgcg ctcgcgctcg aagatgcgcg
     3301 ggaggtattc gtgcggccag cggcggatgc cgggaatgct cgagccgtcc gccggctgca
     3361 gcccgacgat gcggatcgcg ggattcattt ccttcaggta gcgcgagacg cccatgatcg
     3421 tgccggtggt acccatcgag ctgacgaagt gcgtgatgcg tccgtggctc tggcgccaga
     3481 tctccgggcc ggtggcctcg taatgcgcga gcgggttgtc ggggttggcg aactggtcga
     3541 gcacgcggcc cctgccctcg gactgcatgc gcagcgcgag gtcacgcgcc gcctccatgc
     3601 cgccctcgcg cggcacgtcg atcagctcgg cgccgaaggc gcgcatgacc tggcggcgct
     3661 cctcgctcat gtgcgcgggc atgatcagca ccatgcggta gccacggatc gccgcggcca
     3721 tcgcgagcgc gatgccggtg ttgccgctgg tggcctcgat cagcgtgtca ccggggcgga
     3781 tctcgccgcg ctcctcggcg cgacggatca tgctgagcgc gggccggtcc ttcaccgagc
     3841 ccgcgggatt gttgccctcg agcttgagga ggatggtgtt gttcacgccg gcctgcatgc
     3901 gctgcagccg caccagcggc gtctcgccga cgctggcttc cacggtgggg aactccacgt
     3961 gcgccggggc tgcagcggcg ctcgccgcgg gatcgttctg gtgtcggtac atgctgcgtc
     4021 gcacccactc ggatcaaagg cgcatcatac gtgcctgctc cgcgcgggca aacgcacttg
     4081 cgctgataag ctgaaaaccc gtcgctatat cgcgtcggca ggcggagcgt ggcgcgcccg
     4141 gaagtaacca cggctgtgca ggggcctggc gcccagcaat tgcgccagcg cctcgcgcat
     4201 cagccgcttg gcgtagcgac gcgtttcggg cccggaaaaa tcctcgcgcc cgatctgcgc
     4261 cagcacccgg cccgggtagg cgcgcggatc ggccgacgcc ggcgcggcat ggaatcctcc
     4321 gtcgtggctg agcaggtatt ggcctgccgg ctcgagcgcc tgaccggtcg aactgtcgtg
     4381 cacgaattcc atgccgtagc cgagctcgcg cagcagcagc aattcgaagc gacgcagcag
     4441 cggttcgagg tcctcggccg acgcgatccc gggcagcagc gcggcgtagg catcgaaaag
     4501 ggcgctatgg gcgtcgtgct gcatcagcag ccgtatcagc agctcgttca cgtacagcgc
     4561 cgtgtagagc cgctcgcccg cgatcgcggg cgcgggcgcc acggcctcgg cggcgctcag
     4621 cgtcttcagg tcggcgcgtc cggaccacga caccagcagc ggctggaagg gctggcagga
     4681 ggccccggag ccgcgccgcc cgccgcgcgc accgcgggcg atgacgccga tgcggccgaa
     4741 gtcgcgcgtc agcaggtcga gcagcaggct ggtctcgcgc caggcgcgcc ggtgcagcac
     4801 gaaggcgggt tgccggtcca cacgcgaacc cgcggcgctc accgtgacac ccccgccacg
     4861 atcagctttc ttcctggtac ccgaggctct tcagcgcgcg ctcgtcgtcg gaccacccgc
     4921 tcttcacctt gacccagagc ttcagcatga ccttggtgcc gagcagacgc tccatgtcga
     4981 tgcgcgcctc ggcgccgatg agcttcagcc gcgccccggc gcggccgatc acgatggctt
     5041 tctgccccgc cttctcgacc aggatcagcg cgctgatatg gatggtcttg ccttcctggc
     5101 ggaattcctc gatctgcacc gcgttggcgt agggcagttc ctcgccgagc tggcgcatca
     5161 gtttttcgcg cacgatctcc gccaccatga agcgctcgct gcggtcggtg atctggtccg
     5221 gcgggaacag gtgcggtgcc tcgggcagca gcgtctggat ctcgcgctcg agcgcctcga
     5281 ggttgtgccc tttcagcgcc gacaccggca cgatggccgc gaacggatac ttagcctgca
     5341 gcgccgcgat gtgcggcagc agcgcgtcct tctgctcgat ctggtcggtc ttgttgatga
     5401 tcagcagcac cggcttgccc gcggtgcgca cggcggccag cacctgctcg tcaggctcca
     5461 gccactgcag ccggtcgacc agcatcagca cgaggtccac gtcgcgcacc gcgctgagcg
     5521 cggcgcggtt catggcgcgg ttgatcgcct tgccctcgcc ctggtgcagg ccgggggtgt
     5581 cgacgtagac gatctgcgcc gccccgtgcg tgctcacgcc gaggatgcga tggcgcgtgg
     5641 tctgcggctt gcgcgaggtg atgctgatct tctgcccgag caggtggttc agcagggtgg
     5701 acttgcccac gttggggcga cccacgatcg ccaccatgcc acatcgttcg gtgctcatcg
     5761 ctattgctcc gctccttgca ggcgggcgag cgccgtgcgc gccgcccgct gttccgccgc
     5821 acgcttggtg ccggcctgcg cctccaccgg caccgccagg ccgcgcacat ggcacagcac
     5881 gcgaaaatgc tgctgtcccg gcgcgccgct ggtggcggcg agctcgtagc gcggcaacgg
     5941 tgctcgccgc gcctgcaggt actcctgcag ctcggtcttg ggatccttgt gcgagatgcc
     6001 gggctcgagc ccggcgaggc gcgcggcata ccagcgccgc acgacgccga gcgccgcatc
     6061 ggtgcccgca tcgaggcaga tggcgccgag cagcgcctcg accgcgtcgg cgagtatcga
     6121 gtcgcgctcg cgtcccccgc tggaggcttc gccggcgccc aggcgcagca cggcgcccag
     6181 gcccagctcg cgaccgatgt cggccagcgt ctcgccgcgc accaggctga cccgcaggcg
     6241 gtgcaactgc ccttcgcgca gcgcagggta gcgcgcatag agctcggccg cgatgccgag
     6301 gttcagcacc gcgtcgccga ggaactcgag gcgttcgttg ttgccggcgc cgaagctcgc
     6361 gtgcgtcagc gccagttcga gcagctcgcg gcgcgcgaat tcgtgcccga tacgggactg
     6421 caggcgcgcg atatcggggc cagcgtcacg aggcaccggg aatgacgatc tccacgcggt
     6481 tcttgtcgaa gttcacgatg gcgtccacgt tgcccatcac gtgcgtgcgg gcctcgtagt
     6541 tggcgtcgat gatcaggtgg gtcttggtct tctcgacctt caggcacggc ctggccttgt
     6601 cgcggcagac ggcctggatg gcctcgatct ggttgacgtc gaacttgcgc tccaccgcca
     6661 ggcggatttc ctgcgtcgac ttgcgcccca gctcgggctc gttctgcagc gcctgcacgg
     6721 tcgcgcggac cgtgtagttg ttgatgtaga gcggcgccag gctgaacccc acggtcagca
     6781 ggaatccccc cacgagcacg atgaccatcc actgccagat ggtgaaaccc gcctggcggc
     6841 tcctgctgga actgttcatc gtcgcttgct ccccgtatcc gaccggatcg cgctcaattg
     6901 tagacgcatc tagcggatgg tgcccacgcg gtcgaagctc ggcatggtga agaagctgcc
     6961 ccagtgcatc cagatcgcga aggccttgcc gacgatgttg cgctccggca cctcgcccca
     7021 gacgcggctg tcgctgctgt tgtcgcggtt gtcgcccatc atgaaatagt gtccgggcga
     7081 taccgtatgc tcgaaatcgt ccgggatgcg ccgcatcagt tccttgcgca ccacgtgcgg
     7141 cacccccgcg agttcctctt cgaggatctg gtagctcggg tgcagcggcg gcagctgcgc
     7201 gatcaggctc tgcggcatct gctcgccgtt cacgtacagc accttgtcac ggtaggcgac
     7261 gcggtcgccc ggcagcccga tgacgcgctt gatgaaatag cgcttgtcgt tcggcgggaa
     7321 gaacaccatc acgtcgccgc gctgcggcgt gccgacgtcg aggaccttgg tgttcagcac
     7381 cgggagccgg atgccgtagg caaacttgtt caccaggatg tagtcgccga ccagcagcgt
     7441 gggcaccatc gacgaggacg ggatctggaa cggctcgacg aggaagctgc gcagcaccag
     7501 cacgatcagc agcacgggca ggaaggagcg cgcgtactcc acgggcacgg gctccttcag
     7561 cgccttgccg gcctcggcgg catagccgag cgcatcggcg ctgccggcct cggaccagcg
     7621 cgggaagcgc gcgcgcagcg cggcgtcgcg gcgcctgcgc gcgggacgca gccacagcac
     7681 gtccagcgcc catatcacca gggcgacaaa agtcagcgct accagaatca gcggaaaatc
     7741 gatgtccatc gccccacctg tcaactgtcg actttcagca ccgcgaggaa cgcggcctgc
     7801 gggatctcga cgcgccccac ctgcttcatg cgcttcttgc cttctttctg cttttccagc
     7861 agctttttct tgcgcgtcac gtcgccgccg tagcacttgg cgatcacgtt cttgcgcagc
     7921 gccttcacgg tctggcgcgc gatgatgtgc ccgccgatgg cagcctggat cgccacgtcg
     7981 aacatctgcc gcgggatgag ctcgcgcatc ttctcgacca gcacccgccc gcggcttgcc
     8041 gcctggtcac ggtgcacgat gatcgccagc gcatcgacgc gatcgccgtt gatgagaacg
     8101 tcgagccgca ccaggttcgc ggcctggaaa cgctcgaagc tgtagtcgag cgaaccgtag
     8161 ccgcgggtca gcgacttgag gcggtcgaag aagtccagca ccacttccga cagtggcagc
     8221 tcccaggtga gcgagacctg gcgcccgagg aactgcatgt ccttctgcac gccgcgcttc
     8281 tcgacgcaga ggttgatcgc cgcgcccacg tagtcctgcg gcaccaggat gttcgcgcgc
     8341 acgatgggct cgcgcatctc ctcgaccatg ccggggtccg gcaggcgcga ggggttgtcg
     8401 accatgatgg tctcgccccg cgcggtgagc acctcgtaga tcacggtggg ggccgtggtg
     8461 atcaggtcga ggtcgtattc gcgttccagc cgctcctgga tgatttccat gtgcagcgtg
     8521 ccgagaaagc cgcagcggaa gccgaaaccg agcgcgtcgg aggtctctgg ctcgtagaac
     8581 agcgaggcgt cgttcagcgt cagcttggcc agcgcgtcgc ggaaatcctc gtagtcctcg
     8641 gagttcacgg tgaacagccc ggcgtagacc tgcggcttga ccttctggaa gccgggcagc
     8701 gcgggcgcgc tgtcggcggt cttcaggtgc gtgatggtat cgcccaccgg cgcgccgtgg
     8761 atgtccttga tgccggcgac gatgaagccg acctcgcccg ccgacagcga ctggcgctgc
     8821 agacgcttgg gcgtgaacac gccgacctgg tccacctcgt gggcgcggcc gatggacttg
     8881 gtgacgatgc ggtcgcgcac gcgcagcgtg ccgttcatga cccgcaccag cgacaccacg
     8941 ccgaggtagg tgtcgaacca ggaatcgatg atcagcgcct gcagcggcgc gtcgggatcg
     9001 ccctcgggcg gcgggatcac ccgcaccagt tcctcgagca gttcgtcgat gccgagcccg
     9061 ctcttggcgc tgacgcggca ggcgtgcgag gcatcgatgc cgatgatgtc ctcgatctcc
     9121 ccggccacgc gctcgggctc ggcctggggc aggtccatct tgttgaggac cggcaggact
     9181 tccagcccct gctcgatggc cgtgtagcag ttggcgaccg attgcgcctc cacgccctgc
     9241 ccggcatcga ccaccagcag cgcgccctcg caggccgcca gcgagcgcga gacctcgtag
     9301 gagaagtcca cgtgcccggg ggtgtcgatg aagttgagct ggtaggtctc gccgtcccgt
     9361 gccttgtagg ccagcgtgac gctctgcgcc ttgatggtga tgccgcgctc gcgctcgata
     9421 tccatcgagt cgagcacctg ctcctgcatc tcgcggccgc tcaggcctcc gcagacctgg
     9481 atgaaacggt cggcgagcgt cgatttgcca tgatcgatgt gcgcgatgat ggaaaaattg
     9541 cgtatccggt tgagggctgt cacccgcgct tcaccacgtt ccgatccaga gtctgggggt
     9601 tccttgacga ccgcgtactc tacgtgatga cggctgcgtt gaccgcccgc aaaagcgcgc
     9661 aatactaccc gatcgccccc ggatcccgag cggcggcgcg tccctgcgcc gccgcgaggc
     9721 tcgcctcagt cctcgatgcg gatggccacg aagccggcct gcccgccgcg cagcagtcgc
     9781 accggcacgt gcgtgcccga aggcagcgcg gccacgattt cgctgaaccg cctggcgtca
     9841 cgaacggact cgctgccgat cagcgtgatc acgtcgcccg ggcgcagacc cgcgcggtcg
     9901 ccggccttgc cgcgcgccac ctcgcgcacg accacgccgc cgttgatgcg caactgcgcc
     9961 ttctgctgct ggtcgaggtc atcgatgacc agcccgatgc gcccgccggt cgcgctggcc
    10021 tgggccaccg cgcccgtttc ctgcgcgccc tcgagctcac ccaccgtgat gcgcaggtcc
    10081 gtcttcttgc cctcgcggat caccttcatg gccgagcgtg tgcccggctt gaccatgccc
    10141 acgatatgcg gcagatcggc ggactcgttg acgggctgcc catcgaattc gatgaccaca
    10201 tcgccaacgc gcacaccggc cttctcggcc gggccgcccg gctcgacctg cgagatcagg
    10261 gcgccggcgg gcttgtccag cccgaagctc tcggccaggg cacggtccac gtcctggatg
    10321 cccacgccca gccagccgcg c
//
LOCUS       DAORUQ010000114        10310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_6841_length_10310_cov_6.703657, whole genome shotgun sequence.
ACCESSION   DAORUQ010000114 DAORUQ010000000
VERSION     DAORUQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6841_length_10310_cov_6.703657"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLW13_10590"
     CDS             <1..722
                     /locus_tag="PLW13_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQY70867.1"
                     /translation="IKVNDRTDLEEGLQSPIVKFLPVEVRRQLLDRVGAVDGDLIFFG
                     ADRARVVNEALGALRCKLGADLELYTCDWAPLWVVDFPMFERDDKGRWSPLHHPFTRP
                     ACDPQALIDDPGAAISQAYDMVLNGTELGGGSIRIHTSEMQQSVFRILGIGEAEAREK
                     FGFLLDALRYGCPPHGGLAFGLDRLVMLMTRSASIRDVIAFPKTQSASCLLTEAPGVV
                     DAQQMKDLHIRLRLPAAEAAG"
     gene            761..1279
                     /gene="ruvC"
                     /locus_tag="PLW13_10595"
     CDS             761..1279
                     /gene="ruvC"
                     /locus_tag="PLW13_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQY70868.1"
                     /translation="MPVILGVDPGSRITGFGVIRAEGARLEYLSSGCIKVAEEELPGR
                     LKRIFECLSEIIEEHRPTEFAIEQVFMAKSAGSALKLGQARGAAIVAAVRLGLPVYEY
                     SARRIKQSVVGTGAAGKEQVQHMVRTLLKLPAAPQADAADALAAALCHAHTRQSLGRM
                     PGASAARRGRVR"
     gene            1302..1925
                     /gene="ruvA"
                     /locus_tag="PLW13_10600"
     CDS             1302..1925
                     /gene="ruvA"
                     /locus_tag="PLW13_10600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQY70869.1"
                     /translation="MIGRIHGIILEKQAPEVLIKAHGVGYEVQAPLSTFYRLPPVGQE
                     VVLHTHFVVREDAQQLYGFWHRDDRSLFRALIRVSGVGPKLALAILSGMDAEEFARCV
                     QRNDASALVKLPGVGKKTAERLMIEMRDVLREWRPDLALAKGEAPAPDIAEVSRNRLV
                     QDAESALIALGYKPQEAARAVSAALDEDVRESEELIRRALKGFASAR"
     gene            1936..2985
                     /gene="ruvB"
                     /locus_tag="PLW13_10605"
     CDS             1936..2985
                     /gene="ruvB"
                     /locus_tag="PLW13_10605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQY70870.1"
                     /translation="MIETDRLVAPDGKQNEDALDRAIRPRALADYVGQPVVREQMEIF
                     VAAARGRREALDHTLIFGPPGLGKTTLAHIIANEMGVSLKSTSGPVLEKAGDLAALLT
                     NLERGDVLFIDEIHRLSPHVEEALYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVQRLEFYSVADLAWIVGRSGSILGIEIDDKGAREIARRARG
                     TPRIANRLLRRVRDFAQVRHDGRITAAIADQALDMLSVDQQGFDHMDRRLLLAMIEKF
                     DGGPVGVDSLAAAISEERGTIEDVLEPYLIQQGYMLRTPRGRMVTRAAYLHFGLQPRG
                     ALATPDMLADAGESA"
     gene            3000..3455
                     /gene="ybgC"
                     /locus_tag="PLW13_10610"
     CDS             3000..3455
                     /gene="ybgC"
                     /locus_tag="PLW13_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HQY70871.1"
                     /translation="MDQELRLPVRVYIEDTDAGGIVFYVNYLKYFERARTEFMRRLGF
                     AKAALLGGEFMFVVHSMDVQYLRPAQLDDELAAGARVVEARGARLVFAQRVLRGAEEI
                     CRARVTVACVNSATHRPCMIPPRLREALVAAGALAQPPDTRQQSMEIPA"
     gene            3452..4150
                     /gene="tolQ"
                     /locus_tag="PLW13_10615"
     CDS             3452..4150
                     /gene="tolQ"
                     /locus_tag="PLW13_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HQY70872.1"
                     /translation="MNQELSLWALVAEASITVQMVMFVLFMASVVSWGMIVQRALLLR
                     RAKMQFAEFEQTFWSGIDLGQLYRDGSSRLEANGQIEGVENLFRAGFKEFTRLRQQPG
                     IESDAIMEGAQRAMRVAVSREIEYLERHLPFLATVGSTSPYVGLFGTVWGIMHSFRGL
                     ANMHQATLATVAPGISEALVATAMGLFAAIPAVIAYNRYSAQVDNLVANYDTFSEEFS
                     SILHRQAHAGRKSA"
     gene            4167..4583
                     /gene="tolR"
                     /locus_tag="PLW13_10620"
     CDS             4167..4583
                     /gene="tolR"
                     /locus_tag="PLW13_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQY70873.1"
                     /translation="MGRRERRRPMAEINVVPYIDVMLVLLIIFMITAPMLMQGVEVEL
                     PDAAAEPIENQEDEPLIVSVRPDGTYYITLGANPEQPEKLDVIKEKVSKILKAKPKTP
                     VLVWGDEKVPYGIVVVLMSELQEAGAKNVGLVTENP"
     gene            4587..5462
                     /locus_tag="PLW13_10625"
     CDS             4587..5462
                     /locus_tag="PLW13_10625"
                     /inference="COORDINATES: protein motif:HMM:NF018252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY70874.1"
                     /translation="MRSLEGYIVPATVTLLLHAALLAALTMNWQSSQEPLVKPAPRHV
                     KARIVSLEKSGAKAAARPAAVPPKPQPPKPEPPKPKPEPPKPKPEPPKPAPAPPPPKP
                     QPKPTPKPQPKPKPVPPKPKPEPPKPDPKVQEEARKKEQVRREAEQRQQQQREQQVRE
                     QKVREQKVREQQRLEQEMALAMAAEDEALAEGAETTTYEEAIAMAIEDNWSRPPSARR
                     DMQVVLRIQLIPTGEVVGVSVLKSSGDQAFDRSAINAVNKATRFPEVAEAPPQVFERR
                     LRSLQLVFRPEDLRQ"
     gene            5462..6754
                     /gene="tolB"
                     /locus_tag="PLW13_10630"
     CDS             5462..6754
                     /gene="tolB"
                     /locus_tag="PLW13_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQY70875.1"
                     /translation="MKRLGVLVLWLFACVTQAAELSIEITRGADNPTSIAVVPFGATP
                     GLLPPTAIDAVVSANLQRSGLFAPMKVSDMIAQPHEQSQVFYNDWRLLGSDYLVIGRM
                     SRDAAGLYRVQFDLVDVLRQSLLLQETVNGSENQLRDIAHYISDKVYEKLTGVKGAFS
                     TKVAYVSVAQTGNRQNFRLIKADADGERAQMILESPEPILSPAWSPDGRQLAYVSFED
                     RRPGIYRQVIATGEREKLTSFPGLNGAPSWSPDGRRLAMVLSKDDNPEIYMMDLATRQ
                     LTRLTNHFAIDTEPSWMPDGRSIVFTSDRGGKPHIYQLDVATRDLKRLTFEGVYNSRP
                     RPMPDGSGIVYVHRSEAGGPFHIALQNLQRNTVRVLTETSYDESPSVAPNGSMIIYAT
                     KRLGRGILAVVSVDANVKYFLPSAAREVREPAWSPFMR"
     gene            6797..7324
                     /gene="pal"
                     /locus_tag="PLW13_10635"
     CDS             6797..7324
                     /gene="pal"
                     /locus_tag="PLW13_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQY70876.1"
                     /translation="MSNKLVRVTSAIFLAVLVAACSGNKAGKGASTADMAGGTAVGDG
                     SAASVGVGYEGGLSPEEMAARAAAGGLQTVFYFDFDQSALAPETRAALDAQASVLRNQ
                     SSAVRLEGHADERGSREYNLALGERRAKAIANYLILQGIDRSRIETVSYGEERPAALG
                     SDEESWSRNRRVELR"
     gene            7338..8162
                     /gene="ybgF"
                     /locus_tag="PLW13_10640"
     CDS             7338..8162
                     /gene="ybgF"
                     /locus_tag="PLW13_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQY70877.1"
                     /translation="MRRFALLTAAVLLAAPALSQVEVVEPGGATTGPVYSAPAAPAQN
                     RRTQSQPSPAATSGSGELFYQLQLLQDEVMKLRGIVEEQGEQLRQLKQQRLDDYISLD
                     KRIGALGGGSAPMPADGAAGNAGAAPVDASAAPTPPPAAAARSAATPQEEQAYQAAYE
                     LVRARKFPDATTAFKSFVGQYPSGDYAGNAHYWLGELYLLDGDSASAKKHFETLLGQF
                     GDNRRVPDAMFKLGRIYHQEGDVARAKDILDRVVNEYAGSDSTAPRLAREYLQQNF"
     gene            8221..8296
                     /locus_tag="PLW13_10645"
     tRNA            8221..8296
                     /locus_tag="PLW13_10645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8254..8256,aa:Lys,seq:ttt)
     gene            8511..8993
                     /locus_tag="PLW13_10650"
     CDS             8511..8993
                     /locus_tag="PLW13_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70878.1"
                     /translation="MSEDYIEILETVYCYATGIDMRDWSLYRSIFLGEVEMDFESWDG
                     IAAHRTSADDLAANIAVYFAGLDATQHSMTNPRVDIRGDRAVCTMYMQATHFLGKGPA
                     AAEFTIGGYYTDHLLKTAAGWKLAAVKLTVLWERGSKEIMQNAFHLGTQRLQQRSAAI
                     "
     gene            9157..9897
                     /locus_tag="PLW13_10655"
     CDS             9157..9897
                     /locus_tag="PLW13_10655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQY70879.1"
                     /translation="MPEIHLDDGRRVPYSIRISPRSRRVRLTLNPRDGLVMVSPPGVD
                     SAELAALATSWRDWIARQLDVMGIADLDARERRRTLPENIALPALGEDWGVLYRYAPA
                     AAPRVREKGASAIVLGGDVSDHPACVSALRRWLMRRARQTLPECLERVAAETRLDFAT
                     VSIRGQRSRWGSCSATGDISLNYQLLFLPPHLLRHVLVHELCHTVELNHSPRFWRTVA
                     RFEPDLAQLREEMRRSWSHVPGWLMAAI"
     gene            10018..>10310
                     /gene="nadA"
                     /locus_tag="PLW13_10660"
     CDS             10018..>10310
                     /gene="nadA"
                     /locus_tag="PLW13_10660"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQY70880.1"
                     /translation="MAAEAELVKAFLRSKHPVELNEAEIADYKARITARLREKDAVLV
                     AHYYTSPLVQALAEETGGCVSDSLQMARFGHDHPAATLVVAGVRFMGETAKILT"
BASE COUNT         1770 a         3376 c         3538 g         1626 t
ORIGIN      
        1 acataaaggt caacgaccgc acggacctcg aggaggggct gcagtcgccg atcgtgaaat
       61 tcctgccggt ggaagtgagg cggcagctgc tcgatcgcgt cggcgcggtc gatggcgacc
      121 tgatcttctt cggcgccgat cgcgcccggg tggtcaacga ggcgctcggt gcgctgcgct
      181 gcaagctcgg cgcggatctg gagctctaca cctgcgactg ggcgccactg tgggtggtcg
      241 acttcccgat gttcgagcgc gacgacaagg ggcgctggag cccgctgcac catccgttca
      301 cgcgcccggc ctgcgatccg caggcgctca tcgacgatcc gggcgcggcg atttcgcagg
      361 cctacgacat ggtgctgaac ggcaccgagc tcggcggcgg ctcgatccgc atccacacca
      421 gcgagatgca gcagtccgtg ttccgcatac tcggcatcgg cgaggcggag gcccgcgaga
      481 aattcggctt cctgctcgat gcgctgcgct acggctgtcc gccgcacggc gggctcgcct
      541 tcggtctcga ccgcctggtg atgctgatga cgcgctcggc ctcgatccgc gacgtgatcg
      601 ccttcccgaa gacgcagagc gcgagctgcc tgctcaccga ggcgccgggc gttgtcgacg
      661 cgcagcagat gaaggatctg cacatccgcc tgcgcctgcc ggcggccgag gcggccggct
      721 gagcgcgctc gcgggtcggt tcgacgccgc ggtgacgcgc gtgccagtga tcctcggggt
      781 agatccgggc tcgcgcatca ccggcttcgg cgtgatccgc gccgaggggg cgcgcctcga
      841 gtacctcagc agtggttgca tcaaggtggc ggaggaagaa ctgccgggac gcctgaagcg
      901 catcttcgaa tgcctcagcg agatcatcga ggagcaccgg cccacggaat tcgcgatcga
      961 gcaggtgttc atggccaaga gcgcgggctc ggcgctgaag ctgggccagg cgcgcggcgc
     1021 ggccatcgtg gcggcggtgc gcctggggct gccggtgtac gagtattcgg ctcggcgcat
     1081 caagcagtcg gtggtcggca ccggggcggc cgggaaggag caggtgcagc acatggtgcg
     1141 cacgctgctg aagctgccgg cggcgccgca ggccgacgcc gccgatgcgc tcgccgccgc
     1201 cctgtgccat gctcacaccc ggcagagcct cgggcgcatg ccgggcgcga gcgcagcgcg
     1261 acgcggtcgc gtgcgctgag gtcgggacga aacagaaaat catgatcgga cgaattcacg
     1321 gcatcattct cgaaaaacag gcgccggaag tgctgatcaa ggcgcatggc gtcggctacg
     1381 aggtgcaggc gccgctcagc accttctacc gcctgccacc ggtcggccag gaagtggtat
     1441 tgcacaccca tttcgtggtg cgcgaggacg cccagcagtt gtacggcttc tggcaccgcg
     1501 acgaccgcag cctgttccga gcgctgatcc gcgtcagcgg ggtggggccg aagctcgcgc
     1561 tcgccatcct ttccgggatg gatgccgagg agttcgcgcg ttgcgtgcag cgcaacgatg
     1621 ccagcgcgtt ggtgaaattg cccggtgtcg gcaagaagac cgccgagcgg ctgatgatcg
     1681 agatgcgcga cgtgctgcgc gaatggcgcc ccgacctggc gctggccaag ggcgaagcgc
     1741 cggctcccga catcgccgag gtgagccgca accgcctggt gcaggatgcc gagagcgcgc
     1801 tgatcgcgtt ggggtacaag ccgcaagagg ccgcgcgcgc ggtatcggcg gcactggacg
     1861 aggacgtccg tgaaagcgag gaattgatcc ggcgcgcgct caagggcttc gcgagcgcgc
     1921 gttgaggaag ccgcgatgat agaaaccgac cgcctggtgg cacccgacgg gaagcagaac
     1981 gaggacgcgc tcgatcgcgc gatccgcccg cgcgcgctcg ccgactacgt gggccagccg
     2041 gtggtgcgcg agcagatgga aatcttcgtc gccgccgcgc gcggccggcg cgaggcgctg
     2101 gaccacacac tgatcttcgg gccgccgggg ctgggcaaga ccacgctggc gcacatcatc
     2161 gccaacgaga tgggcgtctc gttgaagagc acctcggggc cggtgctcga gaaggccggc
     2221 gatctggccg cgctgctgac gaatctcgag cgcggcgacg tgctgttcat cgacgagatc
     2281 caccgcctca gcccgcacgt cgaggaggcc ctgtatccgg ccatggagga cttccagctc
     2341 gacatcatga tcggcgaggg cccggcggcg cgctcgatca agctcgatct gccgccgttc
     2401 acgctggtcg gggcgaccac gcgcgcgggc ctgctgacat cgccgctgcg cgaccgcttc
     2461 ggcatcgtgc agcggctgga gttctattcg gtcgcggatc tggcctggat cgtcgggcgc
     2521 tcggggagca tcctcggcat cgagatcgac gacaagggtg cgcgcgagat cgcccgccgc
     2581 gcgcgcggca cgccgcgcat cgccaaccgc ctgctgcggc gcgtgcgcga tttcgcccag
     2641 gtgcgtcacg acgggcgcat caccgcggcg atcgccgacc aggcgctcga catgctgagc
     2701 gtcgaccagc agggcttcga ccacatggac cgtcgcctgc tgctcgcgat gatcgagaaa
     2761 ttcgacggcg ggccggtcgg cgtggacagc ctggcggcgg cgatcagcga ggagcgtggc
     2821 accatcgagg acgtgctgga gccgtacctg atccagcagg gctacatgct gcgcacgccg
     2881 cgcgggcgca tggtcactcg cgcggcctac ctgcacttcg gactgcaacc gcgcggcgcg
     2941 ctagcgacac cggacatgct cgccgacgcg ggggaatccg cgtgaacgat gtcgcgttca
     3001 tggaccagga actccgcctg cccgtgcggg tctacatcga ggataccgat gccggcggca
     3061 tcgtgttcta tgtcaactac ttgaaatact tcgagcgcgc gcgcacggaa ttcatgcgcc
     3121 ggctcggctt tgccaaggcg gctctgctgg gcggggagtt catgttcgtg gtgcattcga
     3181 tggacgtcca atacctgcgt ccggcgcaac tcgacgacga gctcgcggcc ggcgcgcgcg
     3241 tggtcgaggc gcgcggtgcg cggctggtgt tcgcgcagcg cgtgctgcgc ggcgccgagg
     3301 agatctgccg cgcgcgggtc accgtggcct gcgtgaacag cgcgacgcac cgtccctgca
     3361 tgattccgcc gcgcctgcgc gaggcgctcg tcgccgccgg cgctctggcg caaccgccgg
     3421 atacacgtca acagtcaatg gagattcctg cgtgaaccaa gagttgtcgc tctgggccct
     3481 ggttgccgaa gcgagcatca cggtgcagat ggtcatgttc gtgctgttca tggcctcggt
     3541 cgtgtcctgg ggcatgatcg tgcagcgcgc gctgctgctg cggcgcgcga agatgcagtt
     3601 cgcggaattc gagcagacgt tctggtccgg gatcgatctc ggtcagctct accgcgacgg
     3661 cagcagcagg ctcgaggcga acggccagat cgagggcgtg gagaacctgt tccgcgccgg
     3721 cttcaaggaa ttcacccgtc tgcgccagca gccgggtatc gaatccgatg ccatcatgga
     3781 aggggcgcag cgcgcgatgc gcgtggccgt gtcgcgcgag atcgagtacc tcgagcgcca
     3841 cctgccgttc ctcgccacgg tcggttccac cagtccctat gtcgggctgt tcggcacggt
     3901 gtgggggatc atgcactcgt tccgggggct cgcgaacatg caccaggcca cgctggccac
     3961 ggtcgccccc ggcatctcgg aggcgctggt ggctaccgcg atgggcctgt tcgccgccat
     4021 tccggcggtg attgcctaca accgctactc ggcgcaggtc gacaacctgg tggcgaacta
     4081 cgacaccttc tccgaggagt tctccagcat cctgcaccgc caggcgcatg ccggcagaaa
     4141 gagcgcctga gcggaggatc gtgacgatgg gacgacggga acgccgcaga ccgatggccg
     4201 agatcaatgt cgtgccgtac atcgacgtga tgctcgtgtt gctgatcatc ttcatgatca
     4261 ccgcgccgat gctgatgcag ggagtcgagg tggagttgcc cgatgcggcg gcggaaccga
     4321 tcgagaacca ggaggacgag ccgctgatcg tctcggtcag gcccgacggc acctactaca
     4381 tcaccctggg cgccaacccc gagcagcccg agaagctgga cgtgatcaag gagaaggtga
     4441 gcaagatcct caaggccaag cccaagaccc cggtgctggt gtggggcgac gagaaggtgc
     4501 cctacggcat cgtggtggtg ctgatgagcg aactgcagga agccggcgcg aagaacgtcg
     4561 ggctggtgac cgagaatccc tgaacgatgc gttccctcga gggttacata gtccccgcga
     4621 cggtcacgct gctgctgcac gcggccctgc tggccgcgct cacgatgaac tggcagtcct
     4681 cccaggaacc gctggtgaag cccgcgccgc gtcatgtgaa ggcgcgcatc gtctcgctgg
     4741 agaaatccgg cgccaaggcg gcggcgcgcc cggccgctgt gccgcccaag ccccagccgc
     4801 ccaagcccga accgccgaag cccaagcccg agccgccgaa gcccaagccc gagccgccca
     4861 aaccggcgcc cgcgccgccg ccccccaagc cgcagccgaa gccgacgccc aaaccgcaac
     4921 cgaagcccaa gccggtgcca ccgaagccca agccggagcc accgaagccg gatcctaagg
     4981 tgcaggagga agcgcgcaag aaggagcagg tgcgccgcga agccgagcag cgccagcaac
     5041 agcagcgcga gcagcaggtc cgcgagcaga aagtacgcga gcagaaggtg cgcgaacagc
     5101 agcggctcga gcaggaaatg gcgctggcaa tggcagccga agacgaggcg ctggcggaag
     5161 gagccgagac aacgacctac gaggaggcca tcgcgatggc catcgaggac aactggagcc
     5221 gtccgcccag cgcgcgacgc gatatgcagg tcgtgttgcg gatacaattg atcccgaccg
     5281 gcgaagtcgt cggtgtcagt gtgctgaaga gcagtggcga ccaggcattc gaccgcagtg
     5341 cgatcaatgc ggtcaacaag gccaccaggt ttcccgaagt ggccgaggcg ccgccgcagg
     5401 tgttcgagcg ccgcctgcgc agtttacagc tcgtgttccg tccggaggat ctgcgccagt
     5461 gatgaagcgt ctgggtgtgt tggtgttgtg gctcttcgcg tgcgtgacgc aggcggccga
     5521 gctgagtatc gagatcacgc gcggggccga caatccgacc tctatcgcgg tggtgccctt
     5581 cggcgccacg ccggggctgc tgccgcccac ggcgatcgac gcggtggtct ccgcgaacct
     5641 gcagcgcagc ggtctgttcg cgccgatgaa ggtctcggac atgatcgcgc agccgcacga
     5701 gcagagccag gtgttctata acgactggcg cctgctcggc agcgattatc tcgtgatcgg
     5761 gcgcatgagc cgcgacgcgg cggggctgta ccgcgtgcag ttcgacctgg tcgacgtgct
     5821 gcgccagtcg ctgttgctgc aggagacggt aaacggctcc gagaaccagc tccgtgacat
     5881 cgcccactac atctccgaca aggtctacga aaaactgacg ggggtgaagg gcgcgttctc
     5941 gaccaaggtg gcctacgttt ccgtggcgca gaccggtaac cggcagaact tccgcctgat
     6001 caaggccgat gccgacggcg agcgcgcgca gatgatcctg gagtcgcccg agcccatcct
     6061 ttcgccggcc tggtcgccgg acggtcgcca gctcgcctac gtctcgttcg aggatcgccg
     6121 cccgggcatc tatcgccagg tgatcgcgac cggtgaacgc gagaagctga cctcgttccc
     6181 cggcctcaac ggtgcgccgt catggtcgcc cgacggccgg cgcctcgcga tggtgctatc
     6241 caaggacgac aaccccgaaa tctacatgat ggacctggca acgcggcagc tgacgcgcct
     6301 gaccaaccat ttcgcgattg acaccgagcc gtcctggatg ccggacggcc gctcgatcgt
     6361 gttcacctcg gaccgtggcg gcaagccgca catctaccag ctggatgtgg cgacgcgcga
     6421 cctcaagcgc ctgaccttcg agggcgtcta caactcgcgt ccacggccga tgcccgatgg
     6481 ctcgggcatc gtctacgtgc accgcagcga agccggcggt ccgttccaca tcgccctgca
     6541 gaacctgcag cgcaacacgg tgcgcgtgtt gactgaaacc agttacgacg agtcgcccag
     6601 cgtcgcgccc aacggcagca tgattattta cgcgacgaag cgcctgggta ggggtatact
     6661 cgcggtcgtg tcggtggacg cgaatgtcaa gtatttcctg ccatcggccg cgcgcgaagt
     6721 gcgtgagccg gcgtggtccc ccttcatgcg ttgaacccca ttcagatccc aagaggaaga
     6781 gcgagctgtg agcagcatga gcaataaact ggtgcgcgtg acgagcgcga ttttcctggc
     6841 agtactggtt gcggcctgca gcggcaacaa ggccggcaaa ggcgcgtcga ccgcggacat
     6901 ggccggcggt accgccgtgg gtgacggcag cgcggcctcg gtcggcgttg gatatgaagg
     6961 cggtctctcg cccgaggaga tggcggcgcg cgcagcagcc ggtggtctgc agaccgtgtt
     7021 ctatttcgac ttcgaccagt ctgcgctggc tcccgagacg cgcgccgcgc tggatgcgca
     7081 agcgagcgtg ctgcgcaacc agagcagtgc cgtgcgcctg gaaggccatg cagacgagcg
     7141 cggctcgcgt gagtacaacc tcgcgctggg cgagcgtcgc gccaaggcga tcgcgaacta
     7201 tctgatcctg cagggcatcg atcgcagccg tatcgaaacg gtcagctatg gtgaggagcg
     7261 tcccgcggcg ctgggctcgg acgaagagtc ctggtcgcgc aatcgccggg tcgaactgcg
     7321 ctgagggctg agcatccatg cgccggtttg cgctgctgac ggcagccgtg ctgcttgccg
     7381 cgccggccct ctcccaggtg gaggtggtcg aaccgggcgg cgcgacgaca gggcccgtct
     7441 acagtgcgcc ggctgcaccg gcgcagaacc ggcgtacgca gtcgcaaccg tcaccggcag
     7501 cgacctccgg cagcggcgag ttgttctatc aactgcagtt gctgcaggac gaagtgatga
     7561 agctccgcgg catcgtcgag gagcagggcg agcagctgcg ccagctgaag cagcagcggc
     7621 tcgacgacta catcagcctg gacaagcgca tcggtgcgct ggggggtggc agcgcaccga
     7681 tgcctgcgga cggtgcggcc ggcaacgccg gcgcggctcc tgtcgatgcc tcggccgcgc
     7741 ccactccgcc gccagcggcc gctgcgcgca gcgcggcgac cccgcaggaa gaacaggcct
     7801 accaggcggc ttacgagctg gtgcgtgcgc ggaaattccc cgatgccacc accgctttca
     7861 agagcttcgt cggccagtac ccctcgggag actacgccgg caacgcgcat tactggctgg
     7921 gcgagctgta cctgctggat ggtgacagtg cctcggcgaa gaagcatttc gagacgctgc
     7981 tcgggcagtt tggcgacaac cgcagggtcc cggacgcgat gttcaagctc gggcgcatct
     8041 atcaccagga aggagatgtc gccagggcga aggatattct cgaccgcgtg gtgaacgagt
     8101 acgccggatc ggacagcacg gcgccgcggc ttgcacgcga atacctgcag cagaatttct
     8161 gagccggtca ttaaaggctt gttttttgcg gctcaatccg taccatacgc gtcctctaac
     8221 gggtcgttag ctcagtcggt agagcagttg gcttttaacc aattggtcgc tggttcgaat
     8281 ccagcacgac ccaccagtac aggctcccgg aaagctccgg ggagcgacaa ggaggcctgg
     8341 attttccggg cttttttgtt tctgcctctc gcatagcgcc tgcgcggcgc cgggctcgcc
     8401 agccggcact atcccggccg cagggcaccc cgtcgcggcg caaggcgctt gctgcgggcc
     8461 gccggcctcg accattccgt aaaggcgtcc gtcacggaga gacaccgggc atgtccgagg
     8521 actacatcga aattctcgag accgtctatt gctacgcaac cggcatcgac atgcgggact
     8581 ggtcgctgta ccgatcgatc ttcctgggcg aggtcgaaat ggatttcgag tcgtgggacg
     8641 ggattgccgc ccatcgcacg agcgctgacg atctggctgc caacatcgcg gtttatttcg
     8701 ctggcctgga cgccacgcag cattcgatga cgaacccccg cgtggatatc cggggcgatc
     8761 gcgccgtgtg cacgatgtac atgcaggcta cgcacttcct cggcaagggt cccgccgccg
     8821 ctgaattcac catcggcggc tactacaccg accatctcct gaagactgct gcgggatgga
     8881 agctcgcggc ggtaaagctc acggtgttgt gggagcgtgg cagcaaggaa atcatgcaga
     8941 acgcattcca cctcggcacg cagcgattgc agcaacgctc ggccgcgatc tgaagcgctg
     9001 tcaaaagagg cgatgcgcgg atcgtgcggt cacatcgcgt atttgcgtga ccggcgaccg
     9061 acaaatccat gtcgcgcttt gtcgcggtga accgcgccgc cggagtggcg taaacttgcg
     9121 tggcgaacga ctgccaaacc gcatccagca gggcccatgc cggaaatcca tctcgacgac
     9181 gggcgccgcg tgccctacag catccgtatt tcgccgcgca gccggcgcgt gcgcctcacc
     9241 ctgaacccgc gcgacgggct ggtcatggtc agtccgcccg gcgtcgacag cgccgagctg
     9301 gcggcgctcg cgactagctg gcgcgactgg attgcccggc agctcgacgt gatgggcatt
     9361 gccgacctcg atgcccgtga acggcgtcgc acgctgccgg agaacattgc cctgcccgcg
     9421 ctgggcgagg actggggcgt gctgtaccgc tacgcgcccg cggccgcgcc gcgcgtgcgc
     9481 gagaagggcg cctctgccat cgtgctcggc ggtgacgtgt cggatcaccc ggcctgcgtc
     9541 tcggcgttgc gccgctggct gatgcggcgt gcgcggcaga cgctgccgga gtgcctcgag
     9601 cgcgtcgcgg ccgagacgcg gctcgacttc gccaccgtgt cgatccgcgg tcagcgcagc
     9661 cgctggggca gctgctcggc gacgggcgac atcagcctca actaccagct gctgttcctg
     9721 ccgccgcacc tgctccgcca cgtgctggtg cacgagctct gccacacggt cgaactcaac
     9781 cactcgccgc gattctggcg gaccgttgcc cgcttcgagc ccgatctggc gcagctgcgc
     9841 gaggagatgc gccgctcctg gagtcatgtg ccaggctggc tcatggccgc catctagggt
     9901 cctgccgcgc cgcgcgggcc actgcggtcg ctgtgctatg atcggcccat catcgatttc
     9961 cccacccttc gcgcggaaag cagcatgacc gcaaacgacc cgatgcatct tggccacatg
    10021 gccgccgagg ccgaactggt gaaggcgttc ctgcgctcga agcatccggt ggagctgaac
    10081 gaggccgaga tcgccgatta caaggcacgc atcaccgcgc ggctgcgcga gaaggacgcc
    10141 gtgctcgtgg cgcactacta cacctcgccg ctggtgcagg cgctcgccga ggaaaccggc
    10201 ggctgcgtgt ccgattcgct gcagatggcg cgcttcggcc atgatcatcc ggccgccacc
    10261 ctggtcgtgg cgggcgtgcg tttcatgggc gagaccgcga aaatcctcac
//
LOCUS       DAORUQ010000115        10091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7053_length_10091_cov_6.018733, whole genome shotgun sequence.
ACCESSION   DAORUQ010000115 DAORUQ010000000
VERSION     DAORUQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7053_length_10091_cov_6.018733"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /locus_tag="PLW13_10665"
     CDS             complement(<1..2487)
                     /locus_tag="PLW13_10665"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQY70881.1"
                     /translation="MKFTPLALVLALVMAAFGGILLGGGGALLGLVLGALAGWQLALN
                     ERLRKIEIALASAQPAAPATGHPDAPLRDDGAAAAIRAGAALTPAPPLAAAGTGWQEP
                     ETPAPQPQHVPVEARRHEAPRTPNAVDRAIAWLKDYATTGNVVAKAGVLVLFVGVSFL
                     LKYAVDRNALPIEVRLAGTAAAALALIVTGWRLRARRASFGLILQGGGIGILYLTIFA
                     AARLYQLLPLGFTFVLLLALVALSAALAVLQDARALATLGTIGGFLAPVLTSTGSGSH
                     VALFSYYALLNLGIVGIAWFKAWRVLNLTGFVFTFVIAAAWGQRYYRPEYFASTEPFL
                     VLSFLFYVAVAALFAHRHPPRLRGYVDGTLVFGLPLVCFALQWPLVRDFEFGRAWTAL
                     GMGAVYLLVARWLWRRGLAELRMLNESFLALGVMALSVAIPFAFDGHITAAAWALEGA
                     GLVWIGLRQQRVLARAWGVLLQFGAGFAFWLIASAGAAHTAVLNAQFLGGVFISAGAL
                     ASACLLYRGQGLLRPRERHLHLLLLGWGLLWWFGNGAHEIGLHVPARLETAALLGFGG
                     ASVAALAVAAARLDWKPAQRAALLWLPALAWFVLPDYLAHHAAGPLAGWRAAGWLAAL
                     AALYLALRRAEGSCSRALIGAAHAGALWLVVFVMAWSAHWSINELLPGAHAWAFVAWG
                     LVPAIALALLPAWSRRGGWPAGRFAALYPANAATGLALAALAWVLAANLDAGDPRPLP
                     FLPLLNPLELAQATALLLVLAWWRQVRHIDDFVLHRVPALLPVALGASAFLWLNAATA
                     RAVHHLGGVGFSVHALHRSPVFQGSISVLWA"
     gene            complement(2484..3341)
                     /locus_tag="PLW13_10670"
     CDS             complement(2484..3341)
                     /locus_tag="PLW13_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY70882.1"
                     /translation="MQDNQAQVEFWNGEGGQRWAEHDAQFERTIGPLTAPLLARIAPT
                     GATRALDIGCGCGNQTLALARHLGPGAHVTGVDISAPMLALARRRAAAAAGAAPLEFL
                     LADASTHRFEPQSFGLLFSRFGVMFFADPLQAFRNLHAACAPGARLVFCCWQAPALND
                     WLRLPLQAALRHVPAPAATDPHAPGPFAFADRARVSAILEAAGFGAVQFESLETELCF
                     AEGETLAAATTAMVEAGPVNALLAPLDAEARARALDDIRATLAPHYRDGKMMMRSAAW
                     LVSADRIAS"
     gene            3465..4613
                     /locus_tag="PLW13_10675"
     CDS             3465..4613
                     /locus_tag="PLW13_10675"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQY70883.1"
                     /translation="MPVLLLGALVCFARVAGAAVEPHGAQLMPRAAQSLLIDIARAGE
                     RLVAVGERGHVLYSDDSGQRWTQAPVPVSVMLTAVWFLDAQRGWAVGHDGMIIATDDG
                     GTSWRLQRDGLAEQQARDRAALEQARRDLAAREGSGADAAALEEARLALGEAEDRASQ
                     PAYAPPLMDVWFADADNGIAVGAYGSYLRTSDGGRSWIHDAARIDNPDELHYYAIASG
                     GAGRLLIVGESGAMFRSRDGGASWEALSHAYEGTLFGALAFTPGDAVCAFGLQGTVLV
                     SPDFGDTWQRLDSPTESVLAGGTIARGGEALIVGNVGTMLAARLATGELEDRSRVALR
                     DNLAGVIEAPDGSLVVIGQGGAVRVPAAKAGDSAVVAGEAGAAPAGGG"
     gene            4615..6963
                     /locus_tag="PLW13_10680"
     CDS             4615..6963
                     /locus_tag="PLW13_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQY70884.1"
                     /translation="MAPLDDTVARQEEQFIERLVFGYRPALIAIFLVLTAFFTWQTAQ
                     LRPDASFEKMIPMKHPFVQAMMRHIAELGASGTTIQIAVASTRGDIFDPHYLEVLRKV
                     SDEVFYIEGVDRNRLKSLWTPNVRWTEVTESGFEGSAVMPDTYDGSPESLATLRHNIM
                     RSGQVGRLVANDMRSSIVEFPIFDKDPITGKQLDYHAFSQELETRVRERFQDDSVSIH
                     IVGFAKIVGDLLEGVTAIFLFALITIVITSVLLFLYTRALWSTVAPVACSIMAVIWQM
                     GLLTTLGYGLNAYSILIPFLIFAIGVSHGIQVVNAIGAEAVAGHVPLQAARRGFRGLY
                     IAGMAALLTDAVGFLTMMLIDIEVIQDLGIAASVGVGVIILTNLVLLPVIMSYAGVSP
                     RGLRRIGQRQAADAPHWRLLSRCATPRFAAVSLALALAGYMVGIVGGQNMQIGDLDRG
                     APELHPDSRYNKDNDYLTQNYSTSSDVLVVLVETRPETCFLYDNLALIDRFGWAMQNV
                     PGVESAVSLADVARLVTMGFNEGSLKWATLIRNQRALDTTFGMVPPQLVNADCSLAPV
                     ALFLADHKAETLDTVVKAVQSFAAQHDSDAIRFVLAAGNSGIEAATNQEITKAQTQIL
                     ILVYSVVAALVFLSFRSFTAVICIMVPLGLTSALCNALMAWLGIGVKVATLPVIALGV
                     GVGVDYAIYLYNRLQIFLRQGMKLQDAYYHALRHTGAAVSLTGIALAIGVATWMWSPI
                     KFQSDMGKLLTFMFLWNMIGALWLMPALAHYLIGRKARARTH"
     gene            7009..8592
                     /locus_tag="PLW13_10685"
     CDS             7009..8592
                     /locus_tag="PLW13_10685"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HQY70885.1"
                     /translation="MRRRLAAAALLLAASRAGAFNFETESGLSIDLDTTLTYGVQWRV
                     EDADDNLLRRADPSWDFAQQLSFLSDPDTVIAQNMDDGNNNFDTGLVSNRATALVDIE
                     IEKDNYGAFLRAKAFYDHVYASRRTAMDEAGYLTMNNAPATRRGDFPDETEDEHGAHA
                     EFLDAYAYASFDVGERVLDLRVGSQMINWGEATFFPGINGMQNRFDASVANVPGTEVK
                     EIFLPTGAVYAQLDLTSSLALQTYYQYEWRHTILNGVGSYFSQQDYLGPGAQTFFVGA
                     RGLFGLDPRIPRLADDEPGDQGQYGVALRYSLESGTELAFYYVNAHNKAPSFERQDIA
                     IAGQALPVSYRIRYFDDIRTTGASFSTLVGDTQVNGEVSYRANTPFADAAGDPEREEL
                     LQAQVGFTQVVRPTALWDDLTVVGELVGVDVMGRSGDDLAFDARAFSYAVRADFAYKN
                     VLQALDTNVVLFVMHTLDGTIREAQMVDDAVVAGVTLRGTWLDRIIAELSYANYSGGG
                     FDHWIIDRDNVAFNIKYSF"
     gene            8616..9989
                     /locus_tag="PLW13_10690"
     CDS             8616..9989
                     /locus_tag="PLW13_10690"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQY70886.1"
                     /translation="MRGLLIGGLTLALAAPLALGKVSEEEAARLGTQLTPTGAEAAGN
                     ADGSIPAWSGRIIGVPPGLEFAGPATVYPDPYAGEKPLFSITAQNVEQYAARLSAGQR
                     ALFQTYPKTFRMDVYPTHRDFGVTKDAAQRARHNATHAELYNGDDGVNGYTGGLPFPI
                     PTMGAHPIWNSRLSTPSYNMEGLTDDVVVYPDGNRSSRRGVTYMKVLFSNPDIPIGAD
                     FKSLSKYAAYIWFAVEEPVRDKGSITLIHEPLDYTQTPRSVWRYLPGSRRIRQAPNVG
                     YDTPDGPGGFLTIDDTLGFNGAMDRFEWTLLGRQEMYVPFHSYRFDDPALTRDQLLTV
                     GHANPDYMRYELRRVWVVEATLREGFRHIYGKRRFYVEEDGWHIAITENYDNRGELWK
                     TVMINSLYLFDQKGYDIRSQMFHDLRAGVYNTTRMTNWTRPWNFSAPDPGEAFFTVDN
                     LRKSGKR"
BASE COUNT         1652 a         3503 c         3494 g         1442 t
ORIGIN      
        1 cgcccacagc accgagatgc tgccctggaa taccggcgag cggtgcagcg cgtgcacgct
       61 gaatcccacg ccgccgaggt gatgcacggc gcgcgccgtg gccgcgttca accacaggaa
      121 ggcgctggcg cccagcgcta ccggcagcag cgccggcacc cggtgcagca cgaaatcgtc
      181 gatgtggcgc acctgtcgcc accacgcgag caccagcagc agcgcggtgg cttgtgcgag
      241 ctccagcggg ttcagcaggg gcaggaaagg gagcgggcgc ggatcgccgg catcgaggtt
      301 cgccgccaac acccaggcca gggccgcgag cgcgagcccg gtggccgcgt tggcggggta
      361 cagcgccgcg aagcgtcccg ccggccagcc gccgcgacgc gaccaggcgg gcagcagcgc
      421 cagcgcgatt gccggtacca ggccccaggc gacgaaggcc caggcgtgcg cccccggcag
      481 cagctcgttg atgctccagt gtgcgctcca ggccatcacg aacaccacca gccagagcgc
      541 gccggcgtgc gccgcgccga tcagcgcccg gctgcaactg ccctcggcgc ggcgcagcgc
      601 caggtacagc gcggcgagcg ccgcgagcca gcccgcggcg cgccatccgg ccagcgggcc
      661 agccgcgtga tgcgccaggt agtcgggcag cacgaaccag gcgagggcgg gcagccacag
      721 cagcgcggcg cgttgcgcgg gcttccagtc gaggcgcgcc gcggccacgg ccagcgccgc
      781 gacgctggcg ccgccgaaac cgagcagcgc cgccgtctcg aggcgcgccg gcacgtgcag
      841 tccgatctcg tgcgcgccgt tgccgaacca ccacagcagg ccccagccga gcagcaacag
      901 gtgcaggtgg cgctcgcgcg ggcgcagcaa cccctgaccg cggtacagca ggcaggccga
      961 ggccagcgcg cccgcgctga tgaacacgcc gccgaggaac tgcgcgttca gcaccgcggt
     1021 gtgcgcggcg ccggcgctgg cgatcagcca gaacgcgaag ccggcgccga actgcagcag
     1081 cacgccccag gcgcgcgcca gcacgcgctg ctggcgcagc ccgatccaga ccagcccggc
     1141 gccttccagc gcccaggccg cggcggtgat gtggccgtcg aaggcgaagg ggatcgcgac
     1201 gctgagcgcc atcacgccga gcgcgaggaa actttcgttc agcatgcgca gctcggcgag
     1261 cccgcggcgc cacagccagc gcgcgaccag caggtagacc gcgcccatgc cgagcgcggt
     1321 ccaggcgcgg ccgaactcga agtcgcgcac cagcggccac tgcagcgcga agcacaccag
     1381 tggcagcccg aagaccagcg tgccgtcgac gtagccgcgc aggcgcggcg gatggcggtg
     1441 cgcgaacagc gccgcgaccg cgacgtagaa caggaacgac agcaccagga agggctcggt
     1501 gctggcgaag tactcgggcc ggtagtagcg ctggccccag gcggcggcga tcacgaaggt
     1561 gaacacgaag ccggtgaggt tcagcacccg ccaggccttg aaccaggcga tgccgacgat
     1621 gccgaggttc agcagtgcgt agtagctgaa cagcgccacg tggctgccgc tgccggtgga
     1681 ggtgagcacc ggcgcgagga agccgccgat ggtgcccagc gttgccagcg cgcgcgcatc
     1741 ctgcagcacc gcgagcgcgg ccgacagcgc caccagcgcg agcagcagca cgaaggtgaa
     1801 gccgagcggc aacagctggt acaggcgcgc cgccgcgaat atcgtgaggt agaggatgcc
     1861 gatgccgccg ccctgaagga tcagtccgaa ggacgcgcgg cgcgcgcgca ggcgccagcc
     1921 ggtgacgatc agtgcgagcg ccgctgcggc cgtgcccgcc aggcgcactt ctatcggcag
     1981 cgcgttgcga tcgaccgcgt acttcagcag gaatgagacg cccacgaaca gcaccagcac
     2041 gccggccttg gcgacgacgt tgccggtggt cgcatagtcc ttcagccacg cgatggcgcg
     2101 atcgacggcg ttgggcgtgc gcggcgcctc gtgccgccgg gcctccacgg gcacgtgctg
     2161 cggctgcggc gcgggcgttt cgggttcctg ccagccggtg ccggcggccg caagcggcgg
     2221 agcgggtgtg agcgcggcgc cggcgcgaat ggctgccgcg gcgccgtcat cgcgcagcgg
     2281 tgcatcgggg tgcccggttg ctggcgccgc cggttgcgcg ctcgcgagcg cgatctcgat
     2341 cttgcgcagg cgctcgttca gcgcgagttg ccagcctgcc agcgcgccga gcacgaggcc
     2401 caacagcgcg ccaccgccac cgagcaggat gccgccgaag gcggccatga ccagcgccag
     2461 cacgagcgcg agcggcgtga atttcaactg gcgatccggt ccgccgagac cagccaagcc
     2521 gccgagcgca tcatcatctt cccgtcgcga tagtgcggcg cgagcgttgc gcggatgtca
     2581 tccagcgccc gggcgcgcgc ctcggcgtcg agcggcgcga gcagtgcatt caccggcccg
     2641 gcctccacca tcgcggtggt ggccgcggcg agggtttcgc cctcggcgaa gcagagttct
     2701 gtttccaggc tctcgaactg cacggcgcca aagccggccg cctcgaggat ggcgctgacg
     2761 cgcgcgcggt cggcgaaggc gaagggtccc ggtgcgtgcg gatcggtcgc tgccggcgcg
     2821 ggcacgtggc gcagcgcggc ctgcagcggc aaccgcagcc agtcgttcag cgccggcgcc
     2881 tgccagcagc agaacaccag ccgcgcgccc ggcgcgcagg ccgcgtgcag gttgcggaag
     2941 gcttgcagcg ggtcggcgaa gaacatcacg ccgaagcgcg agaacagcaa cccgaagctc
     3001 tgcggctcga aacggtgtgt cgaggcgtcg gcgagcagga attcgagcgg agcggcaccc
     3061 gcggccgcgg ccgcgcgacg gcgcgccagc gccagcatgg gcgcggagat gtcgacgccg
     3121 gtgacgtgcg cacccgggcc gaggtggcgg gccagcgcca gcgtctggtt gccgcagccg
     3181 cagccgatgt ccagtgcgcg cgtggcgcca gtcggtgcga tgcgcgcgag cagcggcgcg
     3241 gtcagcgggc cgatggtgcg ctcgaactgg gcatcgtgct cggcccagcg ctgcccaccc
     3301 tcgccgttcc agaattcgac ctgtgcctgg ttgtcctgca ttgcgttccc ccccgcgttg
     3361 atgcatatca tgcgcaatcg cgagcggcgt gcctagaatc cgtcgcggaa gttcccggat
     3421 aacacaacag gatcgggatg cgcagttcaa ggaaccatcc gtttttgcct gtcctcctgc
     3481 tgggggcgct cgtgtgcttc gcccgcgtgg ccggcgcagc cgtggagccg cacggtgcgc
     3541 agctgatgcc gcgcgccgcg cagagcctgc tgatcgacat cgcgcgcgcc ggcgagcgcc
     3601 tggtcgcggt gggcgagcgc ggtcacgtgc tctattccga cgattcggga cagcgctgga
     3661 cccaggcgcc ggtgccggtc tcggtgatgc tcacggccgt gtggttcctc gacgcgcagc
     3721 gtggctgggc ggtgggccac gacgggatga tcatcgccac cgatgacggc ggcacgagct
     3781 ggcgcctgca gcgtgacggc ctggcggagc agcaggcgcg cgatcgcgcg gcgctggagc
     3841 aggcgcggcg cgacctcgct gcgcgcgagg gttccggcgc cgacgccgcg gcgctcgaag
     3901 aggcccgtct cgcgctcggg gaagccgagg accgcgcgag ccagccggcc tacgcgccgc
     3961 cgctgatgga cgtctggttc gcggatgcgg acaacggcat cgcggtgggt gcctacggca
     4021 gctacctgcg caccagcgac ggcggacgca gctggatcca cgacgcggcg cgcatcgaca
     4081 accccgacga actccactac tacgcgatcg cctcgggcgg cgccggacgc ttgctgatcg
     4141 tcggcgaatc gggcgcgatg ttccgctcgc gcgatggcgg ggcgagctgg gaggcgctct
     4201 cccacgccta cgagggcacc ctgttcggtg cgctcgcctt cacgccaggc gacgccgtgt
     4261 gcgcattcgg cctgcagggc acggtgctgg tctcgccgga tttcggcgac acctggcagc
     4321 gcctcgacag cccgaccgag tcggtgctgg ccggcggcac catcgcccgg ggtggcgagg
     4381 cgctgatcgt cggcaacgtc gggacgatgc tggccgcgcg tctcgcgacc ggagaactgg
     4441 aagatcgcag ccgcgtggcg ctgcgcgaca atctcgccgg cgtgatcgag gcgcccgacg
     4501 gcagcctggt cgtgatcggg cagggcggcg ctgtccgcgt accggcagca aaggccggcg
     4561 atagcgccgt ggtcgctggt gaagccggtg ccgcgccggc gggaggaggc tgagatggcg
     4621 ccgctcgacg ataccgtcgc gcgccaggag gagcagttca tcgagcggct cgtgttcggt
     4681 taccgcccgg cgctgatcgc gatcttcctg gtgctgaccg ccttcttcac ctggcagacc
     4741 gcgcagctgc gccccgacgc cagcttcgag aagatgatcc cgatgaagca tcccttcgtg
     4801 caggcgatga tgcgccacat cgcggagctc ggcgcctcgg gtaccacgat ccagatcgcg
     4861 gtggcgagca cgcggggcga cattttcgac ccgcactacc tcgaggtgct gcgcaaggtc
     4921 agcgacgagg tcttctacat cgagggcgtg gaccgcaacc ggctcaagtc gctgtggacg
     4981 ccgaacgtgc gctggaccga ggtcacggag tcgggcttcg agggcagcgc ggtgatgccc
     5041 gacacctacg acggctcgcc cgagagcctc gcgacgctgc gccacaacat catgcgctcg
     5101 ggccaggtgg gacgcctggt ggcgaacgac atgcgctcga gcatcgtcga gttcccgatc
     5161 ttcgacaagg acccgatcac cggcaagcag ctcgactacc acgcgttctc gcaggagctg
     5221 gagacgcgcg tgcgcgagcg cttccaggac gacagcgtca gcatacatat cgtcggcttc
     5281 gccaagatcg tcggcgacct gctcgagggc gtgacggcga tcttcctgtt cgcgctgatc
     5341 acgatcgtga tcacctcggt cctgctgttc ctgtacacgc gtgcgctgtg gtccacggtg
     5401 gcgcccgtcg cttgctccat catggcggtc atctggcaga tgggcctgct caccacgctc
     5461 ggttacggcc tcaacgccta ctcgatactg attccgttcc tgatcttcgc gatcggcgtg
     5521 agccatggca tccaggtcgt caacgcgatc ggcgcggagg ccgtggccgg acatgtgccg
     5581 ctgcaggcgg ccaggcgcgg cttccgcggt ctctacatcg cgggcatggc ggcgctgctg
     5641 accgacgccg tcggctttct cacgatgatg ctgatcgata tcgaggtgat ccaggatctg
     5701 ggcatcgcgg cgagcgtggg cgtcggcgtg atcatcctga ccaacctggt gctgctgccg
     5761 gtgatcatgt cctacgccgg cgtctcgccg cgtggcctga ggcgcatcgg gcagcgccag
     5821 gcggcggatg ccccccactg gcgcctgctg tcgcgctgcg ccacgccgcg cttcgcggcg
     5881 gtttcgctgg cgctggcgct ggccggctac atggtcggaa tcgtcggcgg gcagaacatg
     5941 caaatcggcg acctcgatcg cggcgccccc gaactgcatc cggactcgcg ctacaacaag
     6001 gacaacgact acctcacgca gaattactcg acgagctcgg acgtgctggt cgtgctggtc
     6061 gagacccggc ccgagacctg ctttctctac gacaacctgg cgctgatcga ccgcttcggg
     6121 tgggcgatgc agaacgtgcc cggggtcgag tcggcggtgt cgctggccga tgtcgcacgg
     6181 ctcgtgacca tgggcttcaa cgaaggcagc ctgaagtggg ccacgctgat acgcaaccag
     6241 cgcgcgctcg acaccacctt cggcatggtg ccgccgcagc tggtgaacgc agactgcagc
     6301 ctggcgccgg tggcgctgtt cctggccgac cacaaggccg agaccctcga taccgtggtg
     6361 aaggccgtgc agtccttcgc ggcgcaacac gacagcgatg ccatccgctt cgtgctggcg
     6421 gcgggcaact ccggcatcga ggccgccacc aaccaggaga tcaccaaggc gcagacgcag
     6481 atcctgatcc tggtgtactc ggtggttgcg gcgctcgtct tcctcagctt ccgctccttc
     6541 accgcggtga tctgcatcat ggtgccgctc ggcctcacct cggcgctgtg caacgcgctc
     6601 atggcctggc tcggcatcgg cgtgaaggtg gccacgctgc cggtgatcgc gctcggggtg
     6661 ggggtgggcg tcgactacgc gatctacctc tacaaccgcc tgcagatatt cctgcgccag
     6721 ggcatgaaac tgcaggatgc ctactaccac gccctgcgcc acaccggcgc ggccgtgagc
     6781 ctgaccggca tcgcgctcgc catcggggtc gcgacctgga tgtggtcgcc gatcaagttc
     6841 cagtccgaca tgggcaagct gctgaccttc atgttcctct ggaacatgat cggcgcgctg
     6901 tggctgatgc cggcgctcgc gcattacctg atcggtcgca aggcccgcgc taggacgcac
     6961 tgagatgcag caaaggggga atacaccgtg aacccgtcga atcgtgcaat gcgccgccgc
     7021 ttggccgcgg ccgcgctgct gctcgcggcc agccgcgcgg gcgcgttcaa cttcgagacg
     7081 gagtcgggcc tttcgatcga cctcgacacg acgctcacct acggcgtgca gtggcgcgtc
     7141 gaggacgccg acgacaatct gctgcgccgg gccgatccct cctgggactt cgcgcagcag
     7201 ttgtcctttc tctcggatcc cgacaccgtc atcgcgcaga acatggacga cggcaacaac
     7261 aacttcgaca ccggcctcgt ctcgaaccgc gccaccgcgc tcgtggacat cgagatcgag
     7321 aaggacaact acggcgcatt cctgcgcgcc aaggccttct acgaccatgt gtacgcgagc
     7381 cgccgcaccg cgatggacga ggccggctat ctcaccatga acaacgcgcc cgccacgcgg
     7441 cgcggcgatt tccccgacga gaccgaggac gagcacggcg cgcacgcgga gttcctcgat
     7501 gcctacgcct acgcgagctt cgacgtcggc gagcgcgtgc tcgatctgcg cgtgggcagc
     7561 cagatgatca actggggcga ggcgaccttc ttccccggca tcaacggcat gcagaaccgc
     7621 ttcgacgcat cggtcgcgaa cgtgcccggc accgaggtca aggagatctt cctgcccacg
     7681 ggtgcggtgt acgcccagct cgatctcacg tcctcgctcg cgctgcagac ctactaccag
     7741 tacgagtgga ggcacacgat cctgaacggc gtgggcagct acttcagcca gcaggattac
     7801 ctcggacccg gcgcgcaaac cttcttcgtc ggcgcgcgcg ggctgttcgg cctcgatccg
     7861 cgcattccgc gcctcgccga cgacgagccc ggcgaccagg gccagtacgg cgtggcgctg
     7921 cgctacagcc tggagtcggg cacggagctg gcgttctact acgtgaatgc gcacaacaag
     7981 gcgccgagtt tcgagcgcca ggacatcgcc atcgcgggcc aggcgctgcc ggtcagctat
     8041 cgcatacgct atttcgacga catccgcact accggcgcga gcttcagcac gctggtcggc
     8101 gacacccagg tcaatggcga ggtctcgtac cgggccaaca cgccattcgc cgatgccgcc
     8161 ggcgaccccg aacgcgagga actgctccag gcgcaggtgg gcttcacgca ggtggttcgt
     8221 cccaccgcgc tgtgggacga cctcacggtg gtcggcgagc tcgtgggcgt cgatgtcatg
     8281 ggccgcagcg gcgacgacct ggcgttcgac gcgcgggcct tcagctacgc ggtgcgcgcg
     8341 gatttcgcct acaagaacgt gctgcaggcg ctggatacga acgtcgtgct gttcgtcatg
     8401 cacacgctcg acggcacgat ccgcgaggcg caaatggtcg atgacgcggt ggtggccggc
     8461 gtcacgctgc gcggcacctg gctcgacagg atcattgccg agctgagcta tgccaactat
     8521 tccggcggtg gcttcgacca ctggatcatc gaccgcgaca acgtcgcgtt caacatcaag
     8581 tacagcttct gacaggcgca acgggggaga cgggcatgcg tggacttctg atcggcggac
     8641 tgaccctggc gctcgcggcg ccgctggcac tgggcaaggt gagcgaggag gaggcggcca
     8701 ggctcggcac gcagctcacg ccgaccggcg cggaggcggc cggcaacgcc gacggatcga
     8761 ttccggcgtg gagcggcagg atcatcggcg tgccgccggg actcgagttt gcaggccccg
     8821 cgaccgtcta tcccgacccc tacgccgggg agaagccgct gttttcgatc acggcgcaga
     8881 acgtggagca gtacgccgcg cgcctcagcg cgggacaaag ggcgctgttc cagacctatc
     8941 ccaagacctt ccgcatggac gtctatccca cgcaccgcga tttcggcgtg accaaggacg
     9001 cggcgcagcg cgcgcgccac aacgccaccc acgcggagct gtacaacggc gacgacggcg
     9061 tcaacggcta taccggcggc ctgccctttc cgatacccac gatgggcgcc cacccgatct
     9121 ggaactcgcg tctcagcact ccctcctaca acatggaggg gctcaccgac gatgtggtcg
     9181 tctaccccga cggcaaccga tcgtcgcgcc gcggcgtgac gtacatgaag gtcctgttct
     9241 ccaatccgga catccccatc ggcgccgact tcaagtcgct gagcaagtat gccgcctaca
     9301 tctggttcgc ggtcgaggag ccggtgcgcg acaagggctc gatcacgctg atccacgaac
     9361 cgctcgacta cacccagacg ccgcgcagcg tctggcgcta tctgcccgga tcgcggcgca
     9421 tccgccaggc gccgaacgtc ggctacgaca cgcccgatgg ccccggcggt ttcctcacga
     9481 tcgacgacac gctgggattc aacggcgcga tggaccgctt cgagtggacg ctgctcgggc
     9541 gacaggaaat gtacgtgccg ttccacagct accgtttcga cgaccccgcg ctgacgcgcg
     9601 atcaactgct gacggtcggg cacgccaacc cggactacat gcgttacgaa ctgcggcgcg
     9661 tctgggtggt cgaggccacg ctgcgcgagg gcttccgtca catctacggc aagcggcgct
     9721 tctatgtcga ggaagacggc tggcacatcg ccatcaccga gaactacgac aaccgcggcg
     9781 agctgtggaa gacggtgatg atcaactcgc tgtacctgtt cgaccagaag ggctacgaca
     9841 tccgcagcca gatgttccac gatctgcgcg ccggcgtgta caacaccacg cgcatgacca
     9901 actggacccg gccgtggaac ttctcggcgc cggacccggg cgaggccttc ttcaccgtcg
     9961 acaacctgcg caagtccggc aagcgctgac gccttgcggg tcgggcttgt aggtcgggct
    10021 tcagcccgac aatccggcgt atcgcgagcc atcggcgctc gagcacccat cggtgccgcg
    10081 gacacgccgt g
//
LOCUS       DAORUQ010000116         9991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7151_length_9991_cov_5.134561, whole genome shotgun sequence.
ACCESSION   DAORUQ010000116 DAORUQ010000000
VERSION     DAORUQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7151_length_9991_cov_5.134561"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1479)
                     /locus_tag="PLW13_10695"
     CDS             complement(277..1479)
                     /locus_tag="PLW13_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY70887.1"
                     /translation="MAVTNPILATIEKSGAAALGNTLPPRLISADSHVTEPPHCYVDR
                     IDPAFRDRAPKVVRDSDGGDVFVIDGMASAVPLGIVAAAGKDPREIRKSECRVEDLHR
                     GGWDGKARLADQDRDGIAAEIIYPSVGMVICNHPDADYKHACMWAYNRWLQEEFCAAA
                     PDRLFGMGQTAVRSVAETIEEFRRFREMGFRGVMMPGNPATAEDYDHPSFDPLWRAAV
                     ELDLPLGFHILTSRSDGANAIGASGQRQGSAHRGPPQNAAQMLIKSIQDIIGTFIWGR
                     VFERHPALKVVCVEADAGWAPHFAYRMDHGYNRHRFWLKMGDMARTPSEYFYENVYMT
                     FQDDWVALKLVDMLNPRRLMWANDFPHSDSTWPWSRELLLGQTQHLTAQQKAWILHDN
                     AAELYRIA"
     gene            complement(1648..2274)
                     /locus_tag="PLW13_10700"
     CDS             complement(1648..2274)
                     /locus_tag="PLW13_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQY70888.1"
                     /translation="MSADTRRSRRPRAARWLFATCLCAICAALPAWAQPRDFVIDPDH
                     FGLAFRVRHIGYADTLGMFLRASGRFVYDEATRTLLSARVVVDTASVFSNHDKRDEHL
                     RNADFLDVRAHPEAVFEATGYEAAPDGTGRLRGKLTLLGQTRPVELAVRLNKSAVYPF
                     PPGLAKHTLGVSATTTLRRSDWGMDYAVANGLVGDEVHMSFELEAHRQ"
     gene            complement(2264..3058)
                     /locus_tag="PLW13_10705"
     CDS             complement(2264..3058)
                     /locus_tag="PLW13_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQY70889.1"
                     /translation="MRSSPRWLDHSGVIAELALGLLGAVLLSSLVRIGLGAPRAFLVT
                     AVLVYLAMAAVLVWHWPHGNRSLGAANRITLARGVLVCQLAAALVAPSWLASHAHWLV
                     GAAALALACDGLDGWVARRTGTETAFGARFDMELDALLILVLSFCVLVTGKAGSWVLA
                     IGAMRYALVAAMRPWPWLGAPLPESRRRKIVCVWQVASLLCALSPWVPGPLAQALLSV
                     SLALLAWSFAMDVSWLRRASRVPTAPYPEAAPLTPPTRCKEPEHER"
     gene            3146..4126
                     /locus_tag="PLW13_10710"
     CDS             3146..4126
                     /locus_tag="PLW13_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HQY70890.1"
                     /translation="MAQSTEAFWVIAPGQGEIRREPLRDAGPGEVLVRTLFTAISRGT
                     ESLVMRGEVPASEFQRMRAPFQEGEFPFPVKYGYASVGVVESGDADLLGRRVFCLYPH
                     QSRYVVAAGAVTPIPEALPPARAVLAANMETALNAIWDAAPGPGDRITVIGAGVIGAL
                     VAYLCARLPGTDVELLDIDPARSALAGILGCRFRLPEQASGERDLVIHASGHPVGLRT
                     ALRLAGEEATVLELSWYGTREVELPLGGAFHSKRLSLRSSQVGRLPAARVPRWSSGRR
                     LETALALLQDARLDALVNEESPFPLLPASLRRLAADAGGVLCHRIRYEEP"
     gene            4167..4565
                     /locus_tag="PLW13_10715"
     CDS             4167..4565
                     /locus_tag="PLW13_10715"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQY70891.1"
                     /translation="MYSVNVRDHFMIAHSFRGEIFGPAQRLHGATYVVDLTFRRGELD
                     DDGLVVDIGLAASLLKQVLEALDYRNLDEDPAFSGRNTTTEFMARVIFDRVAAATRTG
                     ALGAGGRGLSVLSVTLHESHVAWASYEGAL"
     gene            4562..5611
                     /locus_tag="PLW13_10720"
     CDS             4562..5611
                     /locus_tag="PLW13_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQY70892.1"
                     /translation="MRPSCSFVVAGSPAQLTGGYLYDARIAAGLRELGWRVATHGLEG
                     RFPLPDGEARRAFAECLASHPDGACVVIDGLVLGGLPEVARAHAARLELVALVHHPLA
                     QESGLDPAARRAFAHSERLALACVSRTVVTSAFTARQLAEYGVPAARIHVVEPGVDTA
                     PLAAADHDPARLLCVASLTPRKGHLVLVESLAALRSRAWSCTLVGSTARDPAHAARVI
                     AAIAANDLSRRIALPGELAPPLLREHYLGADLFVLPSLFEGYGMVVSEAVAHGLPVLA
                     THGGALAETLPAGAGVLVPPGDAAALARALQVLLRDTGARARLREGARRARANLRDWH
                     HAAGLFADALAAGPS"
     gene            5608..6519
                     /locus_tag="PLW13_10725"
     CDS             5608..6519
                     /locus_tag="PLW13_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70893.1"
                     /translation="MSEGERGRDPHPDSRFGADWLALRERADADARAPGLTELAGAWL
                     ARRTHRPLVCVDLGAGGGSNPRFLAPRLPGPQAWLLLDHDRDLLERATRDCAMLCDAA
                     GDPVALRVECRDLSVPAPADFAGVDLVCASALIDLAGKTWLDGLALACARSGAAALLS
                     LSVDGEWHFRRAGVADVDADDALARAAFNAHQRRDKGVGGALGPDAPAYLAQQLRLRA
                     CRVRMAPSPWQLAMNRPRDAALAAALLDGWLAAALEQEPASAARLRSWHARRSDALCD
                     PAFALTVGHVDLFACPAQGGPASAAGT"
     gene            6516..7478
                     /locus_tag="PLW13_10730"
     CDS             6516..7478
                     /locus_tag="PLW13_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQY70894.1"
                     /translation="MSAVRASPRRRIGAWLRALSSLLLLAGILAWIRPASLLATVDTV
                     SVAWLGVALLLGVAQVLLSAWRWRLTARLLGMALPLRSAVSEYYRGSFLNQVMPGGVV
                     GDATRAWRHARAQVSGREAWHAVVIERASGQLMLLACALLALPFAPALREVLLAWLAR
                     SGASTAAIAAAAALLAVLAARRAGSAPRRFLRDLSRALLARTAWPRQVLVSLALVCSY
                     IAVYLCCARAIGVQSSALVLAPLVCWVLLAMALPFSLAGWGIREGAAAALWLLAGLPA
                     AEGVAVSLLYGSIVLVSALPGAWFLLAGDRARSTTAARASGAPA"
     gene            complement(7461..8336)
                     /locus_tag="PLW13_10735"
     CDS             complement(7461..8336)
                     /locus_tag="PLW13_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HQY70895.1"
                     /translation="MSRDDTTQAEAWSLILRELRASAGSDATVRDTADGYGIRLPADR
                     SWLSERPVSAAARALLDIFVPLIARGSTLVIAQLGQSLDGRIATANGVSHYINGEAAR
                     THLHRLRAIVDAVVIGVGTLNADDPQLTVRHVEGRNPVRVLLDPEGRARPGSRVFHDG
                     LAPVIHVTASRVGEGGSRMPAGVEPLVLPLHPVHGFDPATLLGALAARGLGRVLVEGG
                     GLTVSRFLQAGVLHRLHIMVAPMLIGSGRPGVCLPPVTSLEGALRPACRVFACGDDSL
                     FDLALDGTSASGGRA"
     gene            8400..9548
                     /locus_tag="PLW13_10740"
     CDS             8400..9548
                     /locus_tag="PLW13_10740"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HQY70896.1"
                     /translation="MRQTERAIFDLHRGLPVLLRDEQGDTLVHPVESLTHASLQQFRA
                     ICPDGVELLLTRQRLAAMSRRIIDESAALAIPLPVSPEQLLRLAAHRGQVLAADTVLG
                     ECSASARVAVRMLRRARALPAAIAARVPAQRAAVAEAEVREGRLLRVRCEEAAALCCG
                     APPRLIRAGEARVPLGEAGDCRFVLFRESGGLHEHVAVLIGDSARWPEAVPVRLHCAC
                     LTGDVFGSQRCDCGEQLQRGIAAISAQGGGILLYLAQEGRGIGLGNKLRVYCLQDEGL
                     DTIDADRTIGFGDDERDFRIAHDILAAIGVTRITLLTNNPAKIAALRSAGTEVANRQP
                     LFGLLTSHNRRYLTAMADRHGHLLHALLDEQEEVRPGVAAGAERDLAS"
     gene            complement(9553..>9991)
                     /locus_tag="PLW13_10745"
     CDS             complement(9553..>9991)
                     /locus_tag="PLW13_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQY70897.1"
                     /translation="RAVVDLAALALRESRGMLVVRANYFQFALPPEALPGAELRHGLH
                     GGALETSMMLHLAPQSVRLDRLPDARSLGETMAAHGQVLGPEAEAGFAWMAEDLNPGG
                     TTGNARLASAALGARLVSHYGRTLARVIEESFDFDLARLGRQG"
BASE COUNT         1375 a         3577 c         3568 g         1471 t
ORIGIN      
        1 cgcgtcgatc tcctcgcgcc cgcgctgcgc gagcccctcg cccaccgcgg tgatccgccc
       61 ccccgtgatc gcgacatcgc cggtgaaggg ctccgccccg gtgccgtcga cgatggtgcc
      121 gccacggatg atcagatctg ctgtttcgcg catgtgccgt gccgttccac ttctgtcgcc
      181 aattatcgat tcatgactgt aggagcggcc catggccgcg aatcatcgcg cgcatggcgc
      241 gctcctacac aaggacgcac cggcgcgcac cgatcatcag gcaatgcggt agagctcggc
      301 cgcgttgtcg tgcaggatcc acgccttctg ctgcgccgtc aggtgctgcg tctgccccag
      361 cagcagctcg cggctccacg gccaggtcga atcgctgtgc gggaagtcgt tggcccacat
      421 caggcggcgc ggattcagca tgtcgacgag cttcagcgcg acccagtcgt cctggaaggt
      481 catgtacacg ttctcgtaga aatactcgct cggcgtgcgc gccatgtcgc ccatcttcag
      541 ccagaagcgg tggcggttgt agccgtggtc catgcggtag gcgaagtgcg gcgcccagcc
      601 cgcgtcggcc tcgacgcaaa ccaccttcag cgccgggtgg cgctcgaaca cgcggcccca
      661 gatgaaggta ccgatgatgt cctggatcga tttgatcagc atctgcgcgg cgttctgcgg
      721 cggaccgcga tgcgccgagc cctggcgctg gccgctggcg ccgatggcgt tggcgccgtc
      781 gctgcggctg gtgaggatgt ggaagccgag cggcagatcg agctccaccg cggcgcgcca
      841 cagcggatcg aagctcgggt ggtcgtagtc ctcggcggtg gcggggttgc ccggcatcat
      901 cacgcccctg aagcccattt cgcggaagcg gcggaactcc tcgatggtct ccgccaccga
      961 gcgcaccgcg gtctggccca tgccgaacag ccgatccggc gccgcggcgc agaattcctc
     1021 ctgcagccag cggttgtagg cccacatgca cgcgtgcttg tagtcggcgt ccgggtggtt
     1081 gcagatcacc atgccgacgg aagggtagat gatctcggcc gcgatgccgt cgcggtcctg
     1141 gtcggcgaga cgcgccttgc cgtcccagcc gccgcggtgc aggtcctcga cgcggcattc
     1201 gctcttcctg atctcgcgcg gatccttacc cgccgccgcc acgatgccca gcggcaccgc
     1261 agaggccatg ccgtcgatca cgaacacgtc gccgccgtcg ctgtcgcgca ccactttcgg
     1321 cgcgcggtcg cgaaaggccg gatcgatgcg gtcgacgtag cagtgcggcg gttcggtgac
     1381 gtgcgagtcg gccgagatca gccgcggcgg cagcgtgttg cccagcgccg cggcaccgga
     1441 cttttcaatg gtcgccagga tcggattcgt cacggccatc gcactgctct cctgtcatgc
     1501 ctgtcgtgga gccttggtca cccgccggcg cgggagcccg tggagattac gccagccgcg
     1561 gcgagaaaat aaatgcctgc gcaggcgttg cgcggcatcg gcaaagcacg cgcgacaggc
     1621 gcgccggaga tgtcgcctta ccaccggcta ctggcgatgg gcctccagct cgaagctcat
     1681 gtgcacctcg tcgccgacca ggccattcgc gaccgcgtag tccatgcccc agtcgctgcg
     1741 gcgcagcgtg gtggtggccg agactccgag cgtgtgcttc gccagcccgg ggggaaacgg
     1801 gtagacggcg gacttgttca gcctgaccgc gagctcgacc ggccgcgtct ggccaagcag
     1861 cgtcagcttg cccctgagcc ggcccgtccc gtccggggcc gcctcgtagc cggtggcctc
     1921 gaataccgcc tccggatgcg cccgcacgtc caggaagtcc gcgttcctca ggtgctcgtc
     1981 gcgcttgtcg tgattgctga agacactggc ggtgtccacc accacgcgcg ccgaaagcag
     2041 cgtgcgcgtg gcctcgtcgt agacgaaccg gccgctggcc cgcaggaaca tgccgagcgt
     2101 atcggcatag ccgatgtgcc tcacccggaa ggcgaggccg aagtgatcgg gatcgatcac
     2161 gaaatcccgc ggctgcgccc acgccggcag cgcggcgcat atcgcgcaga ggcaggtggc
     2221 gaacagccac cgagcggcac gagggcgacg ggaacgacgg gtgtcagcgc tcatgttcgg
     2281 gctccttgca acgagtggga ggggtgaggg gcgctgcctc cggatacggt gctgtcggca
     2341 cgcgcgaagc acgacgcaac cacgacacgt ccatcgcgaa agaccacgcc agcaacgcga
     2401 gcgagacgga caggagcgct tgcgcgagcg gtcccggcac ccatggcgac agcgcgcaga
     2461 gcaggctcgc cacctgccac acgcagacga tcttgcgccg ccggctctcg ggcagcgggg
     2521 cgcccagcca gggccacggc cgcatcgccg ccaccagcgc atagcgcatg gcgccgatcg
     2581 cgagcaccca gctgccggct ttgcccgtca cgagtacgca gaagctgaga acgaggatca
     2641 gcaaggcatc gagttccatg tcgaagcgcg cgccaaaggc ggtctcggtt ccggtgcgac
     2701 gcgctaccca gccgtccagc ccgtcgcagg ccagcgccag tgccgcggcg ccgaccagcc
     2761 agtgcgcatg cgaggcgagc cacgacggcg cgaccagcgc cgccgcgagc tggcaaacca
     2821 gcacgccccg cgcgagcgtg atccggttcg ccgcgccgag actcctgttg ccgtgcggcc
     2881 agtgccacac cagcaccgcg gccatcgcca ggtacacgag cacggcggtc acgaggaaag
     2941 cgcgcggtgc gccgagcccg atgcgcacca gcgacgagag cagcactgcg ccgagcagcc
     3001 ccagcgcgag ctcggcgatt acgccggaat ggtccagcca cctgggagaa gaccgcatgg
     3061 atgtcgggta ctcgcgttgc tgcgtagaag atacactcac cgggcccact gccggcacgc
     3121 cgcacccggc gcaggaatca tacacatggc gcagagcacc gaggcattct gggtcatcgc
     3181 ccccggccag ggcgagatcc gtcgcgaacc gctgcgcgat gcgggcccgg gagaggtgct
     3241 ggtgcgcacg ctcttcacgg ccatcagccg cgggaccgaa agcctggtga tgcgcggaga
     3301 agtgcctgcc agcgagttcc agcgcatgcg cgcgccgttc caggagggcg agtttccgtt
     3361 tccggtcaag tacggctatg ccagcgtcgg tgtggtcgaa tccggcgacg ccgacctgct
     3421 cggtcgccgc gtgttctgtc tctacccgca ccagtcgcgc tacgtggtcg ccgccggggc
     3481 ggtcacgccc atccccgaag cgctgccacc cgcccgcgcc gtgctcgccg cgaacatgga
     3541 aaccgcgctg aacgccatct gggacgcggc tccggggccc ggcgaccgga tcacggtgat
     3601 cggcgcgggc gtgatcggcg cgctggtcgc ttacctgtgc gcgcgccttc cgggcacgga
     3661 tgtcgagctg ctcgacatcg atcccgcgcg cagcgcgctc gccgggattc tcggctgccg
     3721 cttccgcctg ccggagcagg cgagcggcga gcgcgacctg gtgatccacg ccagcggaca
     3781 tcccgtagga cttcgcacgg cgctgcgcct ggccggggag gaggcgaccg tgctggaact
     3841 cagctggtac gggacccgcg aggtggagtt gcctctcggc ggagcgttcc actcgaagcg
     3901 gctgtcgctc aggtcgagcc aggtcggaag gctgccggcg gcgcgggtac cgcgctggag
     3961 cagcggccgc aggctggaga cggcgctcgc gctgctgcag gacgcacggc tggatgcgct
     4021 ggtgaacgag gagagccctt ttcctttgtt gcccgccagc ctgcggcgcc tcgcggcgga
     4081 tgcggggggt gtgctctgcc atcgcatccg ctacgaggaa ccctgaggcg cgtcccgcgt
     4141 cttgtcgccc gcacaggagc cgagccatgt acagcgtcaa cgtccgcgat cacttcatga
     4201 tcgcccacag cttccggggc gagatcttcg gtccggcgca gcgtcttcac ggcgcgacat
     4261 acgtggtgga tctgacattc cggcgcgggg aactcgacga cgacgggctg gtcgtggaca
     4321 tcgggctcgc cgcatcgctg ctgaagcagg tgctcgaggc actcgactac cgcaacctcg
     4381 atgaggatcc cgcgttctcg ggtcgcaaca ccacgaccga gttcatggcg cgggtgatct
     4441 tcgatcgtgt cgcggccgcc acgcgcaccg gggcgctggg cgccggcggg cgcggactga
     4501 gcgtcctgtc ggtcaccctc cacgagtccc acgtcgcctg ggcaagctac gaaggagcct
     4561 tgtgaggccg agctgcagct ttgtcgttgc cggctccccg gcgcagctca ccgggggtta
     4621 cctctacgat gcgcgcatcg ccgcgggcct gcgcgaactc ggctggcggg ttgcgacgca
     4681 cggactcgag ggccgctttc cgctgcccga tggcgaggcg cgccgcgcgt tcgcggagtg
     4741 cctcgcgagc caccccgacg gtgcctgcgt ggtgatcgac ggcctggtgc tcggcggatt
     4801 gcccgaggtc gcccgcgcgc acgcggcgcg gctggagctc gtcgctctcg tgcatcaccc
     4861 gctcgcgcag gagagcggcc tggatccggc cgcgcgccgc gccttcgcgc acagcgagcg
     4921 cctggcgctg gcgtgcgtga gccgcaccgt cgtcaccagc gcgttcaccg cgcgccagct
     4981 cgcggagtac ggggttcccg ccgcgcggat ccacgtggtc gaacccggtg tcgatacggc
     5041 acccctggcc gcggccgacc acgatccggc gcggcttctg tgcgtggcga gcctcacccc
     5101 gcgcaaggga catctggtgc tggtcgagtc gctcgcggcg ctgcgttcgc gggcgtggtc
     5161 ctgcacactg gtcgggagta ccgcgcgcga ccccgcgcat gccgcgcgcg tgatcgcggc
     5221 catcgccgcg aacgacctgt cgcggcgcat cgcattgccc ggcgagctgg ccccgccgct
     5281 gctccgggaa cactacctcg gcgccgacct gttcgtactg ccctcgctgt tcgagggcta
     5341 cggcatggtg gtgagcgagg ccgtcgccca cggcctgccg gtgctcgcca cgcacggcgg
     5401 ggcgctcgcc gagacgctgc ccgccggggc cggcgtgctg gtgccgcccg gcgatgccgc
     5461 cgcgctggcc agggcgctgc aagtgctgct gcgggatacc ggggcgaggg cgcgcctgcg
     5521 cgaaggcgcg cgcagggcac gcgcgaacct gcgggactgg caccacgcgg ccgggctgtt
     5581 cgccgacgca ctcgccgcgg gtccatcgtg agcgagggcg agcgagggcg cgacccccac
     5641 cccgacagcc gcttcggcgc ggactggtta gcgctgcgcg agcgcgccga tgccgacgcc
     5701 cgcgcccccg ggctcacgga actcgcgggc gcctggctgg cccggcgcac acaccgtccg
     5761 ctcgtctgcg tcgacctggg cgccgggggc ggctcgaatc cgcgcttcct cgcgccgcgc
     5821 ctgcccggtc cgcaggcctg gctcctgctc gatcacgacc gggacctgct cgagcgcgcc
     5881 acgcgtgact gcgccatgct gtgcgacgcg gcgggcgatc cggtcgccct gcgcgtggaa
     5941 tgccgggatc tgtccgtccc ggcacccgcg gatttcgccg gcgtggacct cgtgtgcgcg
     6001 tcggcgctga tcgacctggc cggcaaaacg tggctcgacg gcctggcgct tgcctgcgca
     6061 cgctccggcg cggcggcgct gctcagcctg tcggtggacg gcgagtggca cttccggcgc
     6121 gccggtgtgg ccgacgtcga tgcggacgac gcgctcgcgc gggcggcgtt caacgcgcat
     6181 cagcgccgtg acaagggtgt cggcggtgcg ctggggccgg atgcgcccgc ctacctcgcg
     6241 cagcagttgc ggctccgggc ctgtcgcgtt cgcatggcgc cgagcccctg gcagctcgcg
     6301 atgaaccgcc cgcgcgacgc ggcgctcgcc gccgcgttgc tcgacggttg gctggccgcc
     6361 gcactggaac aggagcctgc cagcgccgcg cgcctgcggt cgtggcacgc gcggcgcagc
     6421 gacgcgctgt gcgatcccgc gttcgcgctg accgtgggtc acgtggatct cttcgcctgc
     6481 ccggcgcagg gcgggccggc atccgcggcg ggcacatgag tgcggtgcgc gcttccccgc
     6541 ggcggcgaat cggcgcctgg ctgcgcgcac tgtcgagcct gctgctgctc gcggggattc
     6601 tcgcctggat ccggccggcc agcctgctcg cgacggtgga cacggtctcc gtcgcctggc
     6661 tgggtgtcgc gctgctgctc ggcgttgcac aggtgctgct ctccgcgtgg cgctggcggc
     6721 tcaccgcgcg gctgctgggc atggcgctgc cgctgcgcag cgccgtgagc gagtattacc
     6781 gcggcagttt cctgaaccag gtgatgccgg gcggcgtcgt gggcgacgcg acccgcgcgt
     6841 ggcgccatgc ccgcgctcag gtctcgggcc gggaggcatg gcacgcggtg gtgatcgagc
     6901 gcgcctcggg ccagctcatg ctgctggcct gcgccttgct ggcattgccg ttcgcgccgg
     6961 cattgcgcga ggtactgctc gcatggctgg cgcggagcgg ggcgagcaca gcggcgatag
     7021 ccgctgccgc ggcactgctc gccgtcctcg ccgcgcgccg ggccgggtcc gcgccgcgtc
     7081 gcttccttcg cgatctctcg cgggccctgc tggcgcgaac ggcctggccg cggcaggtgc
     7141 tggtctcgct ggcgctggtg tgcagctata tcgccgtcta tctctgctgc gcccgcgcga
     7201 tcggggtcca gtcttccgcg ctggtgctgg ctccgctggt ttgctgggtg ctgctcgcga
     7261 tggccctgcc gttttcgctg gccggctggg gtatccgcga gggagccgcc gccgcgctgt
     7321 ggctgctggc cggcctgccc gcggccgagg gcgtggcggt gtcgctgctc tatggatcga
     7381 tcgtgctggt atcggcgctt cccggcgcgt ggttcctgct cgccggggac cgggcgcgga
     7441 gcacgaccgc cgctcgcgcc tcaggcgcgc ccgcctgagg cggacgtgcc atcgagtgcc
     7501 aggtcgaaca ggctgtcgtc gccgcaggcg aagacgcggc aggccggacg caacgcgccc
     7561 tccagcgatg tgacgggcgg caggcagaca ccggggcgtc ccgagccgat cagcatcggc
     7621 gccaccatga tgtgcaggcg atgcagcacg cccgcctgca ggaagcgcga gaccgtcagg
     7681 cccccgccct cgaccagcac gcgaccgagg ccgcgtgccg ccagcgcccc gagcagcgtg
     7741 gccggatcga atccgtgcac gggatggagg ggcagtacca ggggttccac gcccgccggc
     7801 atgcgcgaac ctccctcgcc gacgcgggag gcggtcacgt gaatcaccgg agccaggccg
     7861 tcatggaaca cgcggctgcc gggtcgtgcg cgaccctcgg gatccagcag cacacgaacc
     7921 gggttgcgcc cctcgacgtg acggaccgtg agctgcggat cgtcggcgtt cagcgtaccg
     7981 accccgatca ccacggcatc gacgatcgcg cgcaggcggt gcaggtgggt tcgcgccgcc
     8041 tcgccgttga tgtagtgcga cacgccgttc gcggtggcga tgcgaccgtc gaggctctgg
     8101 cccagctgcg cgatcaccag ggtgctgccc cgggcgatca atggcacgaa gatgtcgagc
     8161 agcgcgcggg cggctgccga aaccggccgc tcggacaacc aggaccgatc cgccggcagc
     8221 cggatcccgt acccgtccgc tgtgtcacgg accgtggcgt cgctgccggc gcttgcgcgc
     8281 agctcccgga ggatcagcga ccaggcctcg gcttgcgtag tatcgtcacg cgacattcac
     8341 gagtccttcc gtgcgcttcg cacgggggtg gagagtggga gcaccctggg ggaaagctga
     8401 tgcgacaaac cgagcgagcg atcttcgatt tgcaccgggg cctccccgtc ctgctgcgag
     8461 acgaacaggg agacaccctg gtccatccgg tcgagagcct gacgcatgcg agcctgcagc
     8521 agttccgcgc gatctgcccg gacggcgtcg agctgctgct cacccggcag cggctcgccg
     8581 cgatgtcgag gcgcattatc gacgaatccg ccgcgctggc catcccgctg cctgtttcgc
     8641 cggagcagct gctgcggctc gcggcgcatc gcggacaggt cctcgccgcg gacacggtac
     8701 tgggagaatg ctcggcgagc gccagggtgg cggtgcgaat gctgcggcgc gcacgcgcgc
     8761 tcccggccgc cattgccgca cgggtgcccg cgcaacgcgc cgctgtcgcc gaggccgagg
     8821 tacgcgaggg caggctgctc cgggtgcgct gcgaggaagc tgccgcgctc tgttgcggtg
     8881 ccccgccccg cctgatccgc gcgggcgaag cgcgggtgcc gctcggcgag gccggtgatt
     8941 gccgcttcgt gctgttccgc gagagcggcg gactccacga gcacgtcgcc gtgctgattg
     9001 gcgactccgc gcgctggccc gaggcggtac cggtgcggct gcactgcgcc tgcctcaccg
     9061 gcgacgtgtt cggcagccag cgttgcgact gcggcgagca gctgcagcgc gggatcgcgg
     9121 ccatcagcgc gcagggcggc ggcatcctgc tctacctggc gcaggagggc cggggcatcg
     9181 gactcggcaa caagctgcgc gtctactgcc tgcaggacga gggtctcgac accatcgacg
     9241 ccgaccgcac catcggcttc ggcgacgacg agcgggactt ccgcatagcg cacgacatcc
     9301 tcgcggcgat cggcgtcacc cgcatcaccc tgctcaccaa caacccggcc aagatcgcgg
     9361 cgctgcgctc ggcgggcacg gaggtcgcga accgccagcc cttgttcgga ctcttgacca
     9421 gccacaatcg ccgctacctc accgcgatgg cggaccggca cggtcacctg ctgcatgccc
     9481 tcctcgacga gcaggaggag gtccgccccg gggtcgcggc aggcgccgaa cgcgacctcg
     9541 cctcctgaga attcagcctt gtcgcccgag acgcgccaga tcgaagtcga aactctcctc
     9601 gatgacacgc gccagcgtcc tgccgtagtg actgacaagg cgcgcgccga gcgccgcgct
     9661 ggcgaggcgc gcattcccgg tcgtgccgcc gggattgaga tcctccgcca tccaggcgaa
     9721 gcccgcctcg gcctccggcc ccagcacctg accgtgggcg gccatcgtct cgccgaggct
     9781 gcgtgcgtcc ggaagccggt cgaggcgaac ggactgcggc gcgaggtgca gcatcatgga
     9841 cgtctcgagc gcaccgccgt gcaggccgtg gcgcagttca gcccccggca gcgcctcggg
     9901 cgggagggcg aactggaagt agttcgcgcg caccaccagc atgccccgcg attcccgcag
     9961 cgcgagcgcg gccaggtcca ctaccgcgcg g
//
LOCUS       DAORUQ010000117         9983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7161_length_9983_cov_6.220991, whole genome shotgun sequence.
ACCESSION   DAORUQ010000117 DAORUQ010000000
VERSION     DAORUQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_9983_cov_6.220991"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PLW13_10750"
     CDS             <1..1176
                     /locus_tag="PLW13_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HQY70898.1"
                     /translation="STGCGGCAALLKAVVEHELEARGVSVDRSLCPHFPFTRQQIFHL
                     CKVGEIRTFDELLARHGKGRGCEICKPAVASVLASLWNEHILARPHVALQDTNDNFLA
                     NMQKDGSYSIVPRIPGGEITPEKLIVLGEVGRKYGLYTKITGGQRIDLFGARVEQLPA
                     IWQELVDAGFETGHAYGKALRTVKSCVGSTWCRYGVQDSVGMAIAIEHRYKGLRSPHK
                     LKSAVSGCTRECAEAQSKDFGVIATEKGWNLYVCGNGGMKPRHADLFATDLDDATLIR
                     TIDRFLMFYVRTADRLQRTSVWLDNLEGGIDYLREVILRDKLGICAELEAQMEAVVGT
                     YQCEWKTTLEDPQKLKRFRQFVNAPGARDDNVVFVRERGQIRPAREDERAALIARSA"
     gene            1205..1555
                     /gene="nirD"
                     /locus_tag="PLW13_10755"
     CDS             1205..1555
                     /gene="nirD"
                     /locus_tag="PLW13_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HQY70899.1"
                     /translation="MAMQLVSASKWQRLCTLDDLVPEAGVCALVNGAQVAIFYLPDEP
                     RRLYAIGNRDPIGNANVLYRGLVAEIGGELTVASPLYKQHFSLVSGACLEQPGLSVPV
                     YELALEGNDVLIGA"
     gene            1559..4243
                     /locus_tag="PLW13_10760"
     CDS             1559..4243
                     /locus_tag="PLW13_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY70900.1"
                     /translation="MNAPAVPSCRTTCPYCGVGCGVDARSAAGEIVAVAGTPDHPANR
                     GRLCVKGSALHETGGLAGRLLHPVVDGERATWDQALARVAGGFARVIAEHGPQAVAFY
                     LSGQLLTEDYYVANKLMKGFIGSANVDTNSRLCMASAVAGYKRAFGADAVPCCYEDLE
                     CCDLLVLSGSNAAWTHPVLYQRIEAARRERPGLRVVVIDPRRTASCDIADLHLAIRPG
                     SDAFLFTALLAYLQASGSVDHDFVAAHTEGFEAALAAAGAWDPVRASAALGIAPAALQ
                     TFCDWFAATERTVTFYSQGINQSATGTDKCNAIINCHLATGRIGRAGMGPFSITGQPN
                     AMGGREVGGLANQLAAHMDFTTEAIARVGRFWGAPNMASAPGLKAVELFEAIESGAVK
                     AVWIMATNPVVSLPDADRVRRALARCELVVVSDCIEKTDTTACAHVLLPATGWGEKDG
                     TVTNSERRISRQRALVPAPGEARHDWRIVCDIAARMGFGHAFDYPGPAAIFREHAALS
                     GFENAGTRAFDIGALANLPDDAYEALAPVQWPVNAAHPAGTRRLFGDGRFFTPSGRAR
                     LVAVHAALPCQEGAAEYPFVMITGRVRDQWHTMTRTARAARLLRHIDAPAVSIHPGDA
                     ARLGITNGALVEVGNARGRLCLPAELSDDLKPGELFVPMHWSAQYASAARMGALIAPR
                     TDPVSGQPESKFVPVRVRPVAAACWMQLVVRAPLDVSDADFWVKTPLANGVRYLLAFT
                     ALGPAEAFARVRSLVATSDASALDWLEFADAARGEHRLLGRDGAGVALMLYAARERAR
                     LPEAGWLAESLACGTDDWLLLAGCGSAGAGAGRQVCSCFEVGEQQIARAIRAGANSVR
                     ALGETLRCGTNCGSCVPELKGLIAREARVGRTCFNANT"
     gene            4348..5454
                     /locus_tag="PLW13_10765"
     CDS             4348..5454
                     /locus_tag="PLW13_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY70901.1"
                     /translation="MKLRRWLIVLVICLLVVAALAGIKYQQIRAAIAFGESFPEPSAA
                     VESQRVAASLAGSHIAMIGEIVAPEFLELRNEFEGRVSAVDMVPGSRVRKGDILLQFD
                     ISEESARLEAAKASATLAALNLRRLQQLLTNNSVSRDRVDQARAEADIALANVRQLEA
                     SIAKKTLRAPFDAVVGLHQLEAGEYLAANTAIVTLVGISEFLWVDFNLPPAQGSVDIG
                     ATVAVFVAGHADAALQARVIARDPAVSPQSRNLRYRARLDAAPGIPPGSVVNVHVPTG
                     RSSLIRVPTPAVQRDDMGSYVFVLEGSEDGDGYRARRRPVRLGSEDASVAAVLEGLAP
                     GETIATHGAFKLRDGMRAVVRERVPGAPAAQAAE"
     gene            5459..8560
                     /locus_tag="PLW13_10770"
     CDS             5459..8560
                     /locus_tag="PLW13_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY70902.1"
                     /translation="MTSPAARPRFTDVFVIRPVLAVVISLALIIGGLQAARSIPVLEF
                     PQIESSSLVISTAYVGAPASVVQGFVTEPIERVASTVPGVDYVDSSTTAGSSTVTVWL
                     KLNEDSTAALAELSSRLGQIRYELPAGAEDPAVTVQRADRSGALFYLDVITGEFPRAA
                     VTDYLERHVTPLIGAIDGVQRALVEPGRNPAMRIWIDPARMAAFNISAQEVQAALVAN
                     NVIATIGTSENRLQRLNLIANTTLQTVEDFERLVLRESEGALLYLRDIARVELGEEEG
                     EVSARYDQQDALYLSIWPVPGANEIAIGDDLYEVLETVNATLPDGLRIRIAYDGTLYM
                     RDAIREIFITLAETIVLVGIVVLALMGSVRTALVPLVAIPISLLGAVAAITLAGFSLN
                     LLTILAIVLSVGLVVDDAIVVVENVSRYMRAGKTRIQAALASSRQLLAPIVGMTITLA
                     VVYAPIGFLSGLTGVLFREFAFTLAVAVLISGVVAITLSPIMSAYANHEGGREGRMTR
                     WVNARFDALHRRYGALLARVLRWRAQVLAAGLFFSLLMVPFYLFSQKELAPIEDQSSI
                     NIVVDAPSSASLEYTTGYMHDVIAAVLGTPGVRYVWQVVRSSGAFGGIVFVHPSEREH
                     SVQELLPEVFARMSAITGLRVFPMLFPALPSAGQFDVELVVQSSDSPEQMKRHAVALL
                     EAAHASGRFMFVDTDLKIDMPQLRFVLDRERIAELGLDLDAVSRQLSLLLSGDYVNRF
                     DLKGKAYQVIPMARAQDRADPEALLDLQLRTPAGELVPLSAIASLEREVGPRVLGKFQ
                     QKNAFRILGGVLPGTTKEQGLAALEQAAAQLLPASYSIDYAGESRQLRQEGNTLTGIL
                     GFALLFVYFALAIQFNSFRDPLVVLLGSVPLALAGALMLPFLGLTTINIYSQIGFITL
                     VGLVAKNGILIVEFANHLQSEGFGKLEAIMSAAETRLRPVLMTTAATVLGHFPLVLVT
                     GAGAEARNSIGIVLVAGMLIGTLFTLFVLPCLYLALAKAHAREEVDPQHDAERAAEVS
                     YAS"
     gene            8602..9417
                     /locus_tag="PLW13_10775"
     CDS             8602..9417
                     /locus_tag="PLW13_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70903.1"
                     /translation="MDMGLQGKVAIVTGATANIGRAIALDLASEGARLIAIGRDEEAG
                     ARVVAEARERGAADAHFLAIDLLEPDACERIARFAATTFGGVDVLVNNVGGNVAAGLF
                     AESEPATWQKDIDITLGTVLGVTRAILPLMIGRGGGRIVNLGSTAGTVGDYMLSVYSA
                     AKGAVHAFTRVLAKEVGVHDITVNCVAPYGTLASDPAAFSSGSRFHPEHGFFRQAFAG
                     VDPAERAKMSRSGPLRRTFATPEEVAGAVLYLASSRAGFITGQILHVDGGTLL"
     gene            complement(9443..>9983)
                     /locus_tag="PLW13_10780"
     CDS             complement(9443..>9983)
                     /locus_tag="PLW13_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70904.1"
                     /translation="GPLFKGKRVAVIGGGNSGIEAAIDLAGIVGHVTVLEFMDTLRAD
                     AVLQQKLRSLPNVTVIMNAQTTEVSGDGEKVNGIVYTDRASGASHRIDLEGIFVQIGL
                     LPNTDWLKGTVELARSGEIQVDARGQTSVPGVFAAGDATTVPYKQIIIAMGEGSKASL
                     SAFDYLIRNSLPVAAAAAA"
BASE COUNT         1451 a         3388 c         3557 g         1587 t
ORIGIN      
        1 agcacgggct gcggcggctg cgcggcgctg ctcaaggccg tggtggagca cgaactggaa
       61 gcgcgcggcg tcagcgtgga caggagcctg tgtccgcact tcccgttcac gcgccagcag
      121 atattccacc tgtgcaaggt cggggaaatc cggaccttcg acgaactgct ggcgcgccac
      181 ggcaagggca ggggctgcga gatctgcaag cccgccgtgg cctccgtgct ggcctcgctg
      241 tggaacgagc acatcctggc gcgcccgcac gtggcgctgc aggacaccaa cgacaatttc
      301 ctcgccaaca tgcaaaagga cggcagctac tcgatcgtgc cgcgcatacc cggcggcgag
      361 atcacgcccg agaagctgat cgtgctcggc gaggtcggca ggaagtacgg gctctacacc
      421 aagatcaccg gtggccagcg catcgacctg ttcggcgcgc gcgtcgagca gctgccggcg
      481 atctggcagg agctggtgga tgccggcttc gagaccgggc acgcctacgg caaggcgctg
      541 cgcaccgtga aatcctgcgt cggctccacc tggtgccgct acggcgtgca ggacagcgtc
      601 ggcatggcga tcgcgatcga gcaccgctac aagggcctgc gctcgccgca caagctcaaa
      661 tccgcggtct cgggctgcac ccgcgagtgc gccgaggcgc agtcgaagga tttcggcgtg
      721 atcgccaccg aaaagggctg gaacctgtac gtctgcggca acggcggcat gaagccgcgc
      781 cacgcggatc tcttcgccac cgatctcgac gacgcgacat tgatccgcac catcgaccgc
      841 ttcctgatgt tctacgtgcg caccgccgac cgcctgcagc gcacctcggt gtggctcgac
      901 aacctcgagg gtggcatcga ctacctgcgc gaggtgattc tgcgcgacaa gctcgggatc
      961 tgtgccgagc tcgaggcgca gatggaggcc gtggtcggca cctaccagtg cgagtggaag
     1021 accacgctcg aggatccgca gaaactcaag cgcttccgcc agttcgtcaa cgcgccgggt
     1081 gcgcgcgacg acaacgtggt gttcgtccgg gagcgcggcc agatccggcc cgcgcgcgag
     1141 gacgagcgtg ccgcgctcat cgcccgatcg gcctgagtcc ggtcggtcca cacagcagcg
     1201 aggaatggct atgcaactgg tatctgcgag caaatggcag cggctgtgca cgctggacga
     1261 tctggtgccc gaggcgggtg tctgcgcgct ggtgaacggc gcgcaggtcg cgatcttcta
     1321 cctgcccgac gaaccgcggc ggctgtacgc gatcggcaac cgcgacccga tcggcaacgc
     1381 gaacgtgctc taccgcggcc tggtcgccga gatcggtggc gagctcacgg tggcctcgcc
     1441 gctgtacaag cagcacttca gcctcgtgag cggcgcctgc ctggagcagc ccgggctgag
     1501 cgtgccggtc tacgagctcg cgctcgaggg caacgacgtg ctgatcggcg cctaggcgat
     1561 gaacgcgccg gccgtgccga gctgtcgcac cacctgcccg tactgcggcg tgggctgcgg
     1621 cgtggatgcg cgctcggcgg cgggcgagat cgtcgcggtg gccggcaccc ccgatcaccc
     1681 ggccaaccgc gggcggctgt gcgtgaaggg ttccgcgctg cacgagaccg gcgggctcgc
     1741 ggggcgcctg ctgcacccgg tggtcgacgg cgagcgcgcg acctgggacc aggcgctggc
     1801 gcgcgtggcc ggcggcttcg cgcgcgtgat cgccgagcac gggccgcagg cggtagcgtt
     1861 ctatctctcg gggcagctgc tcaccgagga ctactacgtc gccaacaagc tgatgaaggg
     1921 cttcatcggc agcgccaacg tcgataccaa ctcgcgcctg tgcatggcct cggcggtggc
     1981 gggctacaag cgcgccttcg gcgccgacgc ggtgccgtgc tgctacgagg atctcgagtg
     2041 ctgcgacctg ctggtgctga gcggttccaa tgccgcgtgg acgcatccgg tgctgtacca
     2101 gcgcatcgag gcggccaggc gcgagcgacc gggcctgcgc gtcgtggtga tcgatccgcg
     2161 gcgcaccgcg agctgcgata tcgccgacct gcacctggcg atccgcccgg gcagcgatgc
     2221 gttcctgttc accgcgctgc tggcctacct gcaggcgagc ggtagcgtcg atcacgattt
     2281 cgtggctgcg cacaccgagg gattcgaagc cgcgctcgcg gcggcgggcg catgggaccc
     2341 ggtgcgcgcg agcgcggcgc tgggcatcgc gcccgcggcg ctgcagactt tctgcgactg
     2401 gttcgcggcc accgagcgca ccgtcacctt ctactcgcag ggcatcaacc agtcggccac
     2461 gggcaccgac aagtgcaacg cgatcatcaa ctgtcacctc gccaccgggc gcatcgggcg
     2521 cgcgggcatg ggaccgttct cgatcaccgg ccagcccaac gcgatgggcg ggcgcgaggt
     2581 gggcgggctg gcgaaccagc tcgctgcgca catggatttc acgaccgagg ccatcgcgcg
     2641 cgtggggcgt ttctggggcg cgccgaacat ggcgagcgcg ccgggcctga aggcggtgga
     2701 gctgttcgag gcgatcgaga gcggcgcggt gaaggcggtg tggatcatgg ccaccaatcc
     2761 ggtggtcagc ctgcccgatg ccgaccgcgt gcggcgcgcg ctggcgcgct gcgagctggt
     2821 ggtggtgtcg gactgcatcg agaagaccga taccaccgcc tgcgcgcacg tgctgctgcc
     2881 ggccaccggc tggggcgaga aggatggcac cgtgaccaat tccgagcggc gcatctcgcg
     2941 ccagcgcgcc ctcgtgccgg cgccgggcga agcgcgccac gactggcgga tcgtgtgcga
     3001 tatcgccgcg cgcatgggct tcggtcatgc cttcgactac ccggggcctg ccgcgatctt
     3061 tcgcgagcac gcggctctgt cgggcttcga gaacgcgggc acccgcgcct tcgatatcgg
     3121 tgcgctggcg aatttgccgg atgacgcata cgaggcgctg gcgccggtgc agtggccggt
     3181 gaacgcggcc catcccgcgg gcacgcgccg cctgttcggt gacgggcgct tcttcacgcc
     3241 ctcgggccgg gcgcgcctgg tcgcggtcca cgccgcgttg ccgtgtcagg aaggggctgc
     3301 ggaatatccc ttcgtgatga tcaccggtcg cgtgcgcgac cagtggcaca ccatgacgcg
     3361 taccgcgcgc gccgcgcggc tgctgcgcca catcgacgcg ccggccgtga gcatccatcc
     3421 gggtgatgcc gcgcgcctcg gcatcaccaa cggcgcgctg gtcgaagtcg ggaacgcacg
     3481 cggccggctc tgccttcccg ccgagctgag cgacgacctc aagcccggcg agctgttcgt
     3541 gccgatgcac tggagcgcgc aatacgcctc ggcggcgcgc atgggcgcgc tcatcgcgcc
     3601 gcgcacggat cccgtctcgg gccagcccga gagcaagttc gtgccggtgc gcgtgcgccc
     3661 ggtggcggcg gcctgctgga tgcagctggt ggtgcgcgcg ccactcgatg tcagcgacgc
     3721 ggatttctgg gtgaagacgc cgctggccaa cggagtgcgc tacctgctgg cgttcacggc
     3781 gctggggcct gccgaggcgt tcgcacgggt gcggtccctg gtcgcgacga gcgatgcgtc
     3841 cgcgctcgac tggctggagt tcgcggacgc cgcccgcggc gagcaccggc tgctcgggcg
     3901 tgatggcgcc ggcgtggcgc tgatgctcta cgcagcgcgc gagcgcgcgc gcctgcccga
     3961 ggccggctgg ctcgccgaga gcttggcgtg cggcacggat gactggctgc tgctggccgg
     4021 ttgcggctcg gccggggccg gggcgggccg gcaggtgtgc tcctgcttcg aggtgggcga
     4081 acagcagatc gcgcgtgcga tccgcgccgg cgcgaactcg gtgcgcgcgc tcggggagac
     4141 gctgcgctgc ggcacgaact gcggctcctg cgtcccggaa ctgaagggcc tgatcgcgcg
     4201 cgaggcgcga gtcgggagga cgtgtttcaa cgccaacact tgagcaggag ctgatccggc
     4261 gccaggatcc gcgacgattc ggttaccatg cgcggcgata tcaggccggg ccatcgtccg
     4321 gcgccaccgc atggatcccg ctccgtcatg aaactgcgcc gctggttgat cgtcctcgtc
     4381 atctgcctgc tggtagtggc tgccctggcg ggcatcaagt accagcagat ccgcgcggcc
     4441 atcgccttcg gcgagtcgtt tccggagccc tccgcggcgg tcgagtcgca acgcgtcgcc
     4501 gcgagcctgg cggggagtca tatcgccatg atcggcgaga tcgtcgcgcc cgaattcctg
     4561 gagctgcgca acgagttcga gggccgggtc agcgcggtcg acatggtgcc cggcagccgg
     4621 gtgcgcaagg gtgacatcct gctgcagttc gacatttccg aggagagcgc ccggctcgag
     4681 gcggcgaagg ccagcgcgac gctggcggcc ctgaacctca ggcggttgca gcagctgctg
     4741 acgaacaatt ccgtcagcag ggaccgggtc gaccaggccc gcgccgaggc cgatatcgcg
     4801 ctggccaatg tgcgccagct ggaggcgtcg atcgccaaga agaccctgcg ggcaccgttc
     4861 gacgcggtag tcggcctgca ccagctcgag gccggcgagt acctggcggc gaacacggcg
     4921 atcgtcacgc tggtgggcat cagcgaattc ctgtgggtcg atttcaacct gccgcccgcg
     4981 cagggcagcg tggacatcgg cgccacggtc gcggtgttcg tcgcgggcca tgccgatgca
     5041 gcgctgcagg ctcgcgtgat cgcgcgcgat cccgccgttt cgccgcagtc gcgcaacctg
     5101 cgctaccggg cacgactcga cgcagccccg gggatcccgc ccggatccgt ggtcaatgtg
     5161 catgtgccca cgggccggag cagcctgatc cgggtgccca cgccggccgt gcagcgcgac
     5221 gacatgggca gctacgtgtt cgtgctcgag gggagcgagg acggtgacgg ctaccgcgcc
     5281 cggcgccgcc cggtccgcct gggcagcgag gacgcgagcg tggctgccgt gctcgagggg
     5341 ctcgcacccg gcgagaccat tgccacgcac ggcgccttca agctgcgcga cggcatgcgc
     5401 gccgtcgtgc gcgaacgcgt gcccggcgcg cccgccgcgc aggccgcgga gtagcgccat
     5461 gacatcgccc gcggccaggc cgcgcttcac cgatgttttc gtgatccgcc cggtgctggc
     5521 ggtggtgatt tcgctggcgc tgatcatcgg cggcctgcag gccgcccgtt cgatcccggt
     5581 gctggaattc ccgcagatcg agagctcctc gctggtgatc agcaccgctt acgtgggtgc
     5641 gcctgccagc gtggtgcagg gcttcgtgac ggagccgatc gagcgcgtgg cgtccacggt
     5701 gcccggcgtc gattacgtcg attcctcgac caccgcgggc tcgagcaccg tcacggtctg
     5761 gctcaagctg aacgaggaca gcacggccgc gctcgcggaa ctgtcctcgc ggctcggcca
     5821 gatccgctac gagttgcccg cgggcgccga ggatccggcc gtcacggtgc agcgcgccga
     5881 ccgctccggg gcgctgttct acctcgacgt gatcaccggc gagttcccgc gcgccgcggt
     5941 caccgattat ctcgagcgcc acgtgacccc gctgatcggc gcgatcgacg gagtgcagcg
     6001 cgccctggtc gagcccgggc gcaaccccgc gatgcggatc tggatcgatc cggcgcgcat
     6061 ggccgcgttc aacatcagcg cgcaggaagt gcaggccgcg ctggtcgcga acaacgtgat
     6121 cgccaccatc ggtaccagcg agaaccgcct gcagcgtctc aacctgatcg ccaacaccac
     6181 gctgcagacg gtggaggact tcgagcgcct ggtgctgcgc gagagcgagg gcgcgctgct
     6241 gtacctgcgc gacatcgcgc gcgtggagct cggcgaggaa gagggggagg tcagcgcacg
     6301 ctacgaccag caggatgcgc tgtacctctc gatctggccg gtgccgggcg ccaacgagat
     6361 cgcgatcggc gacgacctct acgaggtgct cgagacggtc aatgcgacgc tgcccgacgg
     6421 cctgcgcatc cgcatcgcct acgacggcac gctgtacatg cgcgatgcga tccgcgagat
     6481 attcatcacg ctggccgaga ccatcgtgct ggtcggcatc gtggtgctgg cgctgatggg
     6541 ctcggtgcgc acggcgctgg tgccgctggt ggccatcccg atctcgctgc tcggcgccgt
     6601 cgcggcgatc acgctggcag gtttctcgct gaacctgctg acgatactgg ccatcgtgct
     6661 ctcggtgggc ctcgtggtgg acgacgcgat cgtggtcgtc gagaacgtct cgcgttacat
     6721 gcgcgcgggg aagacgcgca tccaggccgc gctcgccagc tcgcgccagc tgctggcgcc
     6781 gatcgtcggc atgacgatca cgctggcggt ggtctacgcg cccatcggtt tcctctccgg
     6841 gctgaccggc gtgctgtttc gcgagttcgc cttcacgctg gcggtggcgg tgctgatttc
     6901 cggcgtggtc gcgatcacgc tgtcgccgat catgagcgcc tatgccaacc acgagggcgg
     6961 gcgcgaggga cgcatgacgc gctgggtcaa cgcgcgcttc gacgcgctgc accgccgcta
     7021 cggcgcgctg ctggcgcggg tgcttcgctg gcgtgcccag gtgttggcgg cgggcctgtt
     7081 cttctcgctg ctgatggtgc cgttctacct gttctcgcag aaggagctcg cgccgataga
     7141 ggaccagagc tcgatcaaca tcgtggttga tgcaccttcg agcgcttcgc tcgaatacac
     7201 caccggctac atgcacgacg tgatcgccgc cgtgctcggg acgcccggcg tgcgctatgt
     7261 ctggcaggtg gtgcgatcga gcggggcctt cggcggcatc gtcttcgtgc acccctcgga
     7321 gcgcgagcac agcgtgcagg agctgctgcc ggaggtcttc gcgcgcatga gcgcaatcac
     7381 cggcctgcgc gtgttcccga tgctgttccc ggcgttgccc tcggcgggac agttcgacgt
     7441 ggagctggtg gtgcagtcct cggattcccc cgagcagatg aagcgccatg ccgtggcgct
     7501 gctcgaggcc gcccacgcca gcgggcgctt catgttcgtc gatacggatc tcaagatcga
     7561 catgccgcag ctgcgcttcg tgctcgatcg cgagcgcatc gcggagctcg ggctggatct
     7621 cgacgccgtg agccggcaac tctcgctgtt gctctcgggt gactacgtca accgcttcga
     7681 cctgaagggc aaggcctacc aggtgatccc gatggcgcgg gcgcaggacc gcgccgatcc
     7741 tgaagccctg ctcgacctgc agctgcgcac gcccgccggg gaactggtgc cgctgtcggc
     7801 gatcgcgtcg ctggagcgcg aggtcgggcc gcgcgtgctc ggcaagttcc agcagaagaa
     7861 cgccttccgc attctgggcg gcgtgctgcc gggcaccacc aaggagcagg gactcgcggc
     7921 gctggagcag gccgccgcgc agttgctgcc ggcgtcctac tcgatcgatt acgcgggcga
     7981 atcgcggcaa ctgcgccagg aaggcaacac gctcacgggc attctcggct tcgcgctctt
     8041 gttcgtgtat ttcgcgctcg cgatccagtt caacagcttc cgcgacccgc tggtggtgct
     8101 gctgggctcc gtgccgctgg cgctggccgg tgcgctgatg ctgccgtttc tcggcctgac
     8161 cacgatcaat atctactcgc agatcggctt catcacgctg gtggggttgg tggcgaagaa
     8221 cggcatcctg atcgtggagt tcgccaacca cctgcagtcg gaaggcttcg gcaagctcga
     8281 ggcgatcatg agtgcggcgg aaacccggct gcgaccggtg ctgatgacca ccgcggcgac
     8341 cgtgctcggt cacttcccgc tggtgctggt caccggcgcc ggcgccgagg cgcgcaacag
     8401 tatcggcatc gtgctggtgg cgggcatgct gatcggcacg ctgttcaccc tcttcgtgct
     8461 gccctgcctg tacctggcgc tggcaaaggc ccatgcgcgc gaggaggtcg acccgcaaca
     8521 cgatgccgaa cgggcggccg aggtcagcta cgccagctga gcgcgagccg catcccccga
     8581 caccggcaag gagactcacg catggacatg ggactgcagg gcaaggtggc catcgtcacc
     8641 ggcgccaccg ccaacatcgg tcgcgcgatc gcgctcgatc tcgccagtga aggcgcacgg
     8701 ctgatcgcca tcggccgcga cgaggaggcg ggcgcgcgcg tcgtcgccga ggcgcgtgag
     8761 cggggtgctg ccgacgcaca ctttctcgcg atagacctgc tcgagccgga cgcctgcgag
     8821 cgcatcgcgc gcttcgccgc cacgaccttc ggcggcgtgg acgtgctggt gaacaatgtc
     8881 gggggcaacg tggcggccgg gttgttcgcg gaatcggagc cggcgacgtg gcagaaggac
     8941 atcgacatca ccttgggcac cgtgctgggc gtcacccgcg cgatcctgcc gctgatgatc
     9001 gggcgcggcg gcgggcgcat cgtgaacctg gggtcgaccg ccggaacggt gggcgactac
     9061 atgctgtcgg tctattccgc cgccaagggc gcggtgcacg cctttacccg cgttctcgcg
     9121 aaggaagtgg gggtgcacga catcacggtc aattgcgtcg cgccctatgg caccctggcc
     9181 agcgatccgg cggccttcag cagcggcagc cgctttcacc cggagcacgg gttcttccgg
     9241 caggccttcg ccggggtcga tcccgccgag cgggccaaga tgagccgcag cggcccgctg
     9301 cggcgcacgt tcgccacgcc ggaggaggtc gccggcgcgg tgctgtacct cgcgtccagc
     9361 cgcgccggtt tcatcaccgg ccagatcctg cacgtggacg gcggaacgct gctctaggga
     9421 gcagcgccgc cgggaacccc gatcacgcgg ccgcggctgc ggccaccggc agcgagttgc
     9481 ggatcaggta gtcgaaggcg ctcaggctcg ccttcgatcc ctcgcccatc gcgatgatga
     9541 tctgcttgta gggcacggtc gtcgcgtcgc ccgccgcgaa tacgccgggt accgaggtct
     9601 ggccgcgcgc atccacctgg atctcgccgc tgcgcgccag ttcgacggtg cccttgagcc
     9661 agtcggtgtt gggcagcagt ccgatctgca cgaagatgcc ctccagatcg atgcggtggc
     9721 tggccccgct tgcgcggtcg gtgtagacga tgccgttgac cttctcgccg tcgccgctga
     9781 cctcggtggt ctgcgcgttc atgatcaccg tgacgttggg caggctgcgc agcttctgct
     9841 gcagcaccgc gtcggcgcgc agcgtgtcca tgaactccag caccgtcacg tgcccgacga
     9901 tgccggcgag gtcgatggct gcctcgatac ccgagttgcc gccgccgatc accgccacgc
     9961 gcttgccctt gaacagcggg ccg
//
LOCUS       DAORUQ010000118         9937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7217_length_9937_cov_6.285469, whole genome shotgun sequence.
ACCESSION   DAORUQ010000118 DAORUQ010000000
VERSION     DAORUQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7217_length_9937_cov_6.285469"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..777
                     /gene="adk"
                     /locus_tag="PLW13_10785"
     CDS             136..777
                     /gene="adk"
                     /locus_tag="PLW13_10785"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQY70905.1"
                     /translation="MRVILLGAPGAGKGTQAQFIMEHFGIPQISTGDMLRAAVKEGSE
                     LGRQAKEIMARGGLVSDDIIIALVKERIARDDCRNGFLFDGFPRTIPQAEAMKLAGVK
                     IDHVLEIDVPDAVIIGRLAGRRVHPASGRVYHVEFNPPRAAGRDDVTGDELVHREDDR
                     EETVRQRLGVYHDQTAQLIGYYQGEQASGTRYHRIPGVGAVEEIRARVLAALA"
     gene            803..1837
                     /gene="hemH"
                     /locus_tag="PLW13_10790"
     CDS             803..1837
                     /gene="hemH"
                     /locus_tag="PLW13_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQY70906.1"
                     /translation="MNLPEAAPGGGAPDWNPSSAVILVNLGSPRAATPRGVSAFLAEF
                     LSDRRVVELPPLLWQPILRGIVIPLRARRVAHAYASIWNGDSPLRAISQRQQQALQQH
                     LRQAHGDAAPWVRLAMTYQGPSIAATIADCQRRGIERICVLPLYPQYSATTTGAVFDQ
                     VSRYLLGARSLPELHLVRDYHDATAYIDALAASVREHWREHGRAEHLLLSFHGIPEAN
                     VLKGDPYARQCERTAQLLGARLELPANAFSLAYQSRFGRAKWLMPYTSETLRELARRG
                     VRSVDVMSPAFAADCLETLEEMAVENRAVFLESGGSEYRFIACLNDRDDHVGMMAQIV
                     CGAGSFASRA"
     gene            1991..2365
                     /locus_tag="PLW13_10795"
     CDS             1991..2365
                     /locus_tag="PLW13_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70907.1"
                     /translation="MAIAKKAAAKKAPAAKKKPAAKPRAKKQRASLADRIVQLEKDAE
                     KQLKQLAAALEKRAKADLRSAIDKFEKKWLKERKKSHAKRLTSVQKKAQAKLAALRKK
                     ATAKKKAAAKKKPARKAAAATA"
     gene            2478..3182
                     /gene="tsaB"
                     /locus_tag="PLW13_10800"
     CDS             2478..3182
                     /gene="tsaB"
                     /locus_tag="PLW13_10800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQY70908.1"
                     /translation="MPLLLGIETSTSRCSVAFGDGDTVQELVDERPREHHALILPMVQ
                     ELLRGAGVSLRDLDAIAFGRGPGSFTGLRIAASITQGLAFGAGLPVIPVSSLRALAAD
                     ADAQAGAARGLDHVLVATDAHMGGIYWGLYRRGAQGLCAVRDDALAHADAFAAAQICA
                     LTSTILAGDAWRVYPRILPPQAPWLESAAPTAREVVRLAARMERSAWRSAEEAEPVYV
                     RGASVWKKIAEQPAPF"
     gene            3146..3598
                     /locus_tag="PLW13_10805"
     CDS             3146..3598
                     /locus_tag="PLW13_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70909.1"
                     /translation="MEEDRGAARAILIAPPRTVSLVRGRLLYFAPSRGNAYRGIPGMK
                     IPSGFIGSFQPPSVAELRRTREPQAREAGTGDAAGERFSADGAAAARGELLRQRADAF
                     ESGRVRRIDEPDTYSGPGRSAVAAYLSVARSPVEAAVGAELVGIDVIV"
     gene            3637..4539
                     /locus_tag="PLW13_10810"
     CDS             3637..4539
                     /locus_tag="PLW13_10810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70910.1"
                     /translation="MLAGNNVSRAAFIGLGVMGYHMAGHLARAGYAVTVFNRTRARAL
                     QWSAQFGGSVAATPAQAAAGADAVFCCVGDDDSVRAMVRGPDGALAAMRAGAVLVDHT
                     TASAELARELAAIARETGVGFVDAPVSGGEAGARQGVLTVMAGGEETDFTRVEPLIRS
                     YARAARLLGPAGAGQLCKMVNQICIAGVMAGLAEALHFARRAGLDAEAVIDVISQGAA
                     QSWQMEHRHRTMLAGEYDHGFAVDWMRKDLGIVLSEARRNRASLPATALVDQFYADVQ
                     AMGGGRWDTSSLLARLERTTEDKA"
     gene            4536..5834
                     /locus_tag="PLW13_10815"
     CDS             4536..5834
                     /locus_tag="PLW13_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HQY70911.1"
                     /translation="MNEAARDAFNHELLRFLGASPTPFHAVGALAALLEEGGFRELRE
                     QDAWACEAGGRYFVRRNGSSLIAFVRGRAAAEDAGLRMAGAHTDSPCLRLKPRPELSR
                     SGYLQLGVEVYGGALLNPWFDRDLSIAGRVSYATAGGVAHALIDFREPVAVIPSLAIH
                     LDREANSGRAVNAQNHLPALLALADSEPRDFAAIVLGRLREEHPDCGAERVLDCELSL
                     YDTQPPRLVGLHREFIAGARLDNLLSCFAGVRALMESDGEFDAIMVCNDHEEVGSVSA
                     AGAQGPMLNSLLARLLPERERRERALALSLMISADNAHGVHPNFADKHDGNHGPALNA
                     GPVIKVNASQRYATNSETAALFRWLCEAEGVPVQSFAVRSDMACGSTIGPLTAANVGV
                     RTLDVGVPTFAMHSPRELAGTRDLHALARVLSRFFRRPAL"
     gene            complement(5962..6753)
                     /locus_tag="PLW13_10820"
     CDS             complement(5962..6753)
                     /locus_tag="PLW13_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY70912.1"
                     /translation="MATFSTLDFVIDSGIARITLNRPDAANGISMQLAQELLQASLAC
                     RCSTAVRAVLLTGKGRMFCAGGDLNSFVENAANLEGFIKELTTYLHAAIANFAHMRAP
                     LIVAVNGAAAGAGFSLAACGDLVLAARSAKFSMAYTGAGLVPDGSASYFLPRIIGLRR
                     AQELILTNRRLGAEEALEWGLLTRVVDDAELAAEAEKLAAQIATGPTGAYGAVKKLLA
                     SSFEATLETQMELEGAAIAAAAISRDGQEGVAAFLGKRAPVFSGM"
     gene            6900..7895
                     /locus_tag="PLW13_10825"
                     /pseudo
     CDS             6900..7895
                     /locus_tag="PLW13_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
     gene            7984..8418
                     /locus_tag="PLW13_10830"
     CDS             7984..8418
                     /locus_tag="PLW13_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70913.1"
                     /translation="MIWWILLAVAVAGAVFALRGRAAAPSRKLAPRTGSRGASAAPKL
                     PGRGKAPSEAIDDYRGAMLFPQKDCCEAVLKLRGHTFAEHHAATLPVPGCDRDACGCK
                     LHQVIGRRRGPRRLVPDRRDDVRFKEDRRQGKDRRSGVDVWK"
     gene            complement(8527..8742)
                     /locus_tag="PLW13_10835"
     CDS             complement(8527..8742)
                     /locus_tag="PLW13_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQY70914.1"
                     /translation="MSDHHVYKKVEIVGSSRISIEDAINNALAECSKSIQNLEWFEVV
                     ETRGHVQHGQVGHYQVVLKVGFKIQGS"
     gene            complement(8861..9478)
                     /locus_tag="PLW13_10840"
     CDS             complement(8861..9478)
                     /locus_tag="PLW13_10840"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY70915.1"
                     /translation="MRKLDHDQRRREIAEVAARLIAADGMAALTTRSIARAMGCSIGV
                     LSHYFESKAGIVTAAFAWASARIEQRLARALSRGVSIDNLLPLIEAALPHDRQSRIEW
                     GVRLQLWSHAASDAGAAAVLRNDMERNRALLASVIVQLQHQQEVRPAIDANNVAQMLM
                     DLMHGMGFAALLRLHHAGHDPVAAFNVLVDDLRNGNSGATRAGAA"
     gene            9613..>9937
                     /locus_tag="PLW13_10845"
     CDS             9613..>9937
                     /locus_tag="PLW13_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70916.1"
                     /translation="MKAASLDHDYVHRPDHKDLSHLPGAYGLPYLGMMVPLLKNPYEV
                     IKRHYDRYGAVSRTRLTGQHMVWALGPDYNRELVMDPQQVYSARMGYDAPLGDFFAGG
                     LLVRDF"
BASE COUNT         1528 a         3353 c         3486 g         1570 t
ORIGIN      
        1 ttcgcgccgc agctgagcgg cgcggcgcct cggcttgtta tcgcgcgggg tcctgtgtaa
       61 tgttccccgt cgtcgaattg accgttcatc cgaccggcgc cgcctgcggc acggggtcgt
      121 tacaggagtc catgcatgcg cgtcattctt ttgggtgccc ccggcgcggg aaagggtacc
      181 caggcccagt tcatcatgga gcacttcggt attccgcaga tctccaccgg ggacatgctg
      241 cgcgcggcgg tgaaggaggg atcggaactc gggcgccagg cgaaggaaat catggcgcgc
      301 ggcggactgg tgtcggacga catcatcatc gcgctggtca aggagcgcat cgcgcgcgac
      361 gactgccgca acggcttcct gttcgacggc ttcccgcgca cgatcccgca ggccgaggcg
      421 atgaagcttg ccggcgtgaa gatcgaccac gtgctcgaga tcgacgtgcc cgatgcggtg
      481 atcatcggcc gcctggccgg acggcgcgtg catccggcgt cgggacgcgt ctaccacgtc
      541 gagttcaatc cgccgcgcgc cgcaggacgt gacgacgtga ccggcgacga actggtgcat
      601 cgcgaggacg accgcgagga aaccgtgcgc cagcgcctgg gtgtctatca cgaccagacc
      661 gcgcagctga tcggctacta ccagggcgag caggcgagcg gcacgcgcta tcaccgcatc
      721 ccgggtgtcg gtgcggtcga ggagatccgc gcgcgcgtgc tcgcggcgct cgcctgaacg
      781 cgccgcggcc gcagtcccgc gcatgaacct gcccgaagcg gcgcccggtg gcggcgcacc
      841 ggactggaat ccttcctccg cggtcatcct ggtcaatctc ggctcgccgc gcgccgcgac
      901 gccgcgcggg gtctccgcgt ttctcgccga gttcctctcc gaccgccgcg tcgtcgagct
      961 gccaccgttg ctgtggcagc cgatcctgcg cggtatcgtg attccgctgc gcgccaggcg
     1021 cgtcgcgcac gcctacgcct cgatctggaa cggggattcg ccgttgcgcg ccatctcgca
     1081 gcgccagcag caggccctgc agcagcactt gcgccaggcg catggcgacg ccgcgccatg
     1141 ggtgcggctc gcgatgacct accaggggcc gtcgatagcc gcgaccatcg cggactgcca
     1201 acgccgcggc atcgagcgca tctgcgtgct gccgctgtac ccgcagtact ccgcgacgac
     1261 gaccggcgcc gttttcgacc aggtcagccg ctacctgctc ggtgcgcgct cgctgcccga
     1321 actgcacctg gtgcgcgact accacgacgc caccgcttac atcgacgcgc tcgccgccag
     1381 cgtgcgcgag cactggcgcg agcacgggcg cgccgagcac ctgctgctct cgttccatgg
     1441 catccccgag gcgaacgttc tcaagggtga tccctatgcg cggcaatgcg agcgcacggc
     1501 gcagctgctg ggggcgcggc tggaattgcc tgcgaacgcg ttctcgctcg cataccagtc
     1561 gcgcttcggt cgcgcgaagt ggctgatgcc ctataccagc gaaacgctgc gcgagctcgc
     1621 gcgccgcggc gtgcgctcgg tcgacgtgat gagcccggcg ttcgcggcgg attgcctcga
     1681 aacgctggag gaaatggcgg tggagaatcg cgcggtgttt ctcgagagcg gtggcagcga
     1741 gtaccgtttc atcgcatgcc tcaacgatcg cgatgatcac gtgggcatga tggcgcagat
     1801 cgtctgcggc gcgggttcct tcgcatcgcg cgcatgagtt tcgcggttgc ggtttgcgcg
     1861 agcgcaaaca agtaccgttg attgcgcgtt gcatttcttg tacggcattt gatgaagtgc
     1921 tattttgcat gcgtgatagt tgtgtgttga caacgtcgat ccggataccc agtcaccaag
     1981 gagtgcaatg atggcaatcg ccaagaaagc agcggcaaag aaagctcccg cagcgaagaa
     2041 gaagccggcc gcaaagccgc gcgcgaagaa gcaacgcgcg tcgcttgctg accgcatcgt
     2101 gcaactcgag aaggatgcgg aaaagcagct caagcagctg gctgccgcgc tcgagaagcg
     2161 ggccaaggcg gatctgcgca gcgcaatcga caagttcgag aagaagtggc tcaaggagcg
     2221 caagaagtcg catgccaagc gcctgacgtc ggtgcagaag aaggcacagg ccaagcttgc
     2281 tgcgctgagg aagaaggcaa ccgccaagaa gaaggcggct gcgaagaaga agcctgctcg
     2341 caaggcagca gccgcgaccg cctgagcccg ggttcccggg ccggcaagga agcccgtctc
     2401 ccgcgcaagc gggagggcgg gcttttcatt ttatgtgtcc cgcaattcct ggcggcgacc
     2461 cgtgcagagc atccccagtg ccactgttgc tcggaatcga aacctcgaca tcgcgctgct
     2521 cggtcgcgtt cggtgacggc gataccgtgc aggaactcgt cgacgagcgc ccgcgcgagc
     2581 accacgcgct gatcctgccg atggtgcagg agttgctgcg cggcgcgggc gtttcactgc
     2641 gcgatctcga cgcgatcgcc ttcggccgcg gtcccggctc cttcaccgga ttgcggattg
     2701 ccgcaagcat cacgcagggc ctcgctttcg gcgccggtct gccggtgatt cccgtatcct
     2761 cgctccgggc gctggccgcc gatgctgatg cccaggccgg cgcggcccgc ggcctcgatc
     2821 acgtcctggt cgccacggat gcgcacatgg gcgggatcta ctggggcctg taccgccgcg
     2881 gcgcgcaggg gctctgcgca gtgcgcgacg atgcgctggc gcatgccgac gctttcgccg
     2941 ccgcgcagat ctgcgcgctc acaagcacga ttcttgccgg cgacgcgtgg cgcgtgtatc
     3001 cgcggatcct gccgccgcaa gcgccatggc tggaatcggc cgcgcccacc gcgcgcgagg
     3061 tcgtgcgcct ggcggcgcgc atggagcgct cggcctggcg cagcgccgag gaggcggagc
     3121 cggtctacgt gcgcggggcc tccgtgtgga agaagatcgc ggagcagccc gcgccattct
     3181 gattgcgccg ccgcggacgg tttcgctggt gcgcgggcgg ttgttatact tcgcgccgtc
     3241 tcgaggcaac gcgtaccgtg gcattccagg catgaagatc ccgtcgggtt tcatcggctc
     3301 gttccaaccg ccatccgtgg cggagctgcg ccgcacgcgc gagccgcagg cgcgcgaagc
     3361 cggcaccggt gacgctgccg gcgagcgttt ctctgccgac ggcgccgctg ccgcgcgcgg
     3421 cgagttgctg cgccagcgtg ccgatgcctt cgaatccggt cgcgttcggc gcatcgacga
     3481 gcccgacacg tattccgggc cgggacgcag cgctgtcgcg gcctatctgt ccgttgcccg
     3541 cagcccggtc gaggctgccg tcggtgcgga gctggtcggc atcgacgtca tcgtctgatc
     3601 ggcgcgcccg cgccggccac atccaaagca cagcgcatcc tggcggggaa caacgtgtcg
     3661 cgagctgcat tcatcggtct cggggtcatg ggctaccaca tggcggggca tctcgcgcgt
     3721 gcgggatacg cggtcacggt tttcaaccgc acgcgcgcca gggcgctgca atggtcggcg
     3781 cagttcggcg ggtccgtcgc cgcgactccg gcacaagccg ctgcgggcgc cgatgcggtg
     3841 ttctgctgcg ttggcgacga cgacagcgtg cgcgcgatgg tgcgcggtcc cgatggggcg
     3901 ctcgcggcga tgcgcgccgg cgcggtgctc gtcgaccaca ccaccgcgtc ggcggaactg
     3961 gcgcgcgagc tcgcggcgat tgcacgcgaa accggcgtcg gctttgtcga tgcgccggtc
     4021 tcgggcggcg aggcgggagc acggcagggc gtgctcacgg tgatggccgg gggcgaggag
     4081 accgatttca cgcgggtgga acccctgatc cgcagctacg cgcgtgccgc gcggctgctc
     4141 gggcccgcgg gtgccggcca gctctgcaag atggtcaacc agatctgcat cgccggcgtg
     4201 atggcgggac tggccgaggc gcttcacttc gcgcgccgcg ccgggctcga cgccgaggcc
     4261 gtcatcgacg tgatctcgca gggcgcggcg cagtcgtggc agatggagca ccgccatcgc
     4321 acgatgctcg caggcgagta tgaccacggt ttcgcggtgg actggatgcg caaggacctc
     4381 ggcatcgtgc tgagcgaggc gcgccgcaac cgcgccagcc tgccggcgac ggcgctggtg
     4441 gaccagttct acgccgacgt gcaggcgatg ggcggaggac gctgggatac ctccagcttg
     4501 ctggcccggc tggaacgcac tacggaggac aaggcatgaa cgaggccgcg cgtgacgcgt
     4561 tcaaccacga actgctgcgc tttctcggcg catcgcccac gccgttccat gccgtgggcg
     4621 cgctggctgc gctactggaa gagggcggtt tccgcgagct gcgcgagcag gatgcctggg
     4681 cctgcgaggc gggcggacgc tacttcgtgc gccgcaacgg ctcctcgctg atcgccttcg
     4741 tcaggggccg cgcggccgcc gaggacgcgg gcctgcgcat ggccggggcg cacaccgaca
     4801 gcccttgcct caggctgaag ccgcgcccgg agctgagtcg cagcggttac ctgcagctcg
     4861 gcgtcgaggt ctacggtggc gcgctgctca atccctggtt cgatcgcgac ctctcgatcg
     4921 ccgggcgtgt cagctacgcc acggcgggcg gcgtggcgca tgcgctgatc gattttcgcg
     4981 agccggtggc ggtgattccc agcctcgcca ttcacctcga tcgcgaggcc aactcggggc
     5041 gcgcggtgaa cgcgcagaat cacctgcccg cactgctggc gctggccgac agcgagccgc
     5101 gcgatttcgc ggcgatcgtg ctcgggcgcc tgcgcgagga gcacccggac tgcggcgccg
     5161 agcgcgtgct cgactgcgag ctgtcgctct acgacacgca gccgccacgg ctggtgggcc
     5221 tgcaccgcga gttcatcgcc ggcgcgcgcc tcgacaacct gctcagctgc ttcgccggcg
     5281 tgcgcgcgct gatggagtcc gacggcgagt tcgacgcgat catggtctgc aacgaccacg
     5341 aggaggtggg cagcgtctcg gccgccgggg cgcagggccc gatgctgaac tcgctgctgg
     5401 cgcggctgct gcccgagcgc gagcggcgcg agcgcgcgct ggcgctgtcg ctgatgatct
     5461 cggcggacaa tgcacacggt gtgcacccga acttcgccga caagcacgac ggcaaccacg
     5521 gcccggcgct caacgccggg ccggtgatca aggtcaatgc cagccagcgc tacgcgacca
     5581 acagcgagac cgcggcgctg ttccgctggc tgtgcgaggc cgagggtgtg ccggtgcaga
     5641 gcttcgcggt gcgcagcgac atggcctgcg gcagcaccat cggcccgctg acggcggcga
     5701 acgtgggcgt gcgcacgctc gatgtgggcg tgcccacctt cgcgatgcac tcgccgcgcg
     5761 agctggccgg caccagggat ctccacgcgc tcgcgcgcgt gctgtcgcgc ttcttccgcc
     5821 ggccggcgct gtaggtcggg cttcagcccg acaagggttc ggccgagccg aacccgtcgt
     5881 gccagtcgcc atgaatggcg acatcagcat ttggcaaggc acccgtcggc atgaatgccg
     5941 acccacagca tgaacgccgg cctacatccc gctgaacacc ggcgcgcgct tgccgaggaa
     6001 tgcggcgaca ccttcctggc cgtcgcgcga gatcgcggcc gcggcgatcg ccgcgccctc
     6061 gagctccatc tgcgtctcca gcgtcgcctc gaagctcgag gccagcagtt tcttcaccgc
     6121 gccgtaggcc ccggtgggac ccgtggcgat ctgcgcggcc agcttttcgg cttccgcggc
     6181 cagttcggcg tcgtcgacca cgcgcgtcag cagtccccac tcgagcgcct cctcggcgcc
     6241 gaggcggcga ttcgtgagga tcagttcctg cgcacggcgc aggccgatga tgcgcggcag
     6301 gaaatagctg gcgctgccgt ccggcaccag gccggcaccg gtgtaggcca tcgagaactt
     6361 cgccgagcgc gccgccagca ccaggtcgcc gcaggcggcg aggctgaaac cggcgccggc
     6421 cgcggcgccg ttgaccgcga cgatcagcgg tgcgcgcatg tgcgcgaaat tggcgatagc
     6481 agcgtgcagg taggtcgtga gctccttgat gaagccctcc aggttggcgg cgttctcgac
     6541 gaagctgttg aggtcgccgc cggcgcagaa catgcgtccc ttgccggtca gcagcaccgc
     6601 gcgcaccgcc gtggagcagc ggcaggcgag cgacgcctgc agcagctcct gcgccagctg
     6661 catgctgatg ccgttggcgg catcgggtcg gttcagggtg atgcgcgcga tgccgctgtc
     6721 gatgacgaag tcgagggtag agaacgttgc catttgccgg ggtctccgca gaggggggct
     6781 ggaatgtagg gccggcgcga gcataggcag cgtgcgcgga gcggtcaatc gcggggcgcg
     6841 cattggcgca tcgcggcacc gatacgtatg atacgcggcc tcatccaggg cagtcccgca
     6901 tgaaaaagca gcgcgttctc accggcatca ccaccaccgg cacgccgcat ctcggcaact
     6961 acgtcggcgc catccgtccc gcgatcgcag cgagcctgca gccggacgtg cagtcgttct
     7021 atttcctcgc cgacctgcac tcgatgatca agagccagga cgcgcaggcg ctggggcgct
     7081 ccacgcgcga gatcgccgcg acctggctgg ctctggggct ggataccgag cgcgtggtgt
     7141 tctatcgcca gtcggacgtg ccggagatca ccgagctcac gtggatgctg acctgcatga
     7201 ccgccaaggg cctgatgaac cgcgcacacg cctacaaggc cgcggtgcag gccaaccagg
     7261 aggccgggca ggacgacgat ttcggcgtca ccatgggcct gtacagctac ccggtgctga
     7321 tggcggcgga catcctgatg ttcaacgccg atcgcgtgcc ggtagggcgc gaccagatcc
     7381 agcatgtcga gatggcgcgc gacatcgcgc agcgcttcaa ccaccactac ggcgagcact
     7441 tcgtgctgcc gaccgcggtc gtcgatgagc acgtcgccgt gctcgcgggc ctcgacgggc
     7501 gcaagatgag caagagctac ggcaacacca tcccgctgtt cctgcccgag aagcagttgc
     7561 agaagcacat caaccgcatc aagaccaacc ttcaggagcc cggggagccg aagaatccgg
     7621 acgaatcgac ggtgttccag gtctgggccg cgttcgcgac gcccgggcag cgcgaggaga
     7681 tgcggcgcga gttcgcgaac ggcatcgcgt ggggcgaagc caaacgccgc ctgttcgaac
     7741 tggtcaacgc ggagctgcgc gaaccgcgcg agcgctacga ggcgctgatg gcgcagcccg
     7801 cactgatcga gaaactgctg cgcgagggcg cggagcgcgc gcgcgccttc agccggccgt
     7861 tcatgcagga gctgcgcgcc gcggtcggcc tgcgtcccgt ggacgcatga gccgcgggcg
     7921 ccctggccta tacttcgggc tggtccctgg cgctgcgggg caaatcgggg aggaggtgtc
     7981 ctgatgatct ggtggattct gctcgctgtg gccgtggccg gtgccgtgtt cgcgctgcgc
     8041 ggccgcgccg cggcgccgtc acgcaagctc gcgccacgca ccggatcacg tggcgcgagc
     8101 gccgccccca agctgcccgg gcgaggcaag gcgccgtcgg aggccatcga cgattaccgc
     8161 ggagcgatgc tgttcccgca gaaggattgc tgcgaggcgg tactgaagct gcgcggccac
     8221 acctttgccg agcaccatgc ggcgacgctg cccgttcccg gctgcgaccg cgacgcttgc
     8281 ggctgcaagc tgcaccaggt catcgggcgg cgccgtggac cgcgccgcct cgtgccggac
     8341 cggcgcgacg acgtgcgctt caaggaagac cgccgccagg gcaaggacag gcgctcgggc
     8401 gtggatgtat ggaagtaggg gcgccgagcg cgtcgccgga cgcgggcgtc gatccgcggg
     8461 cttcgacctg aaccgcggct tgccgcgcct aatcgccgcg cccgggcgtg gcacgcagcc
     8521 aggggctcag ctgccctgga tcttgaaacc caccttcagc acgacctggt aatgccccac
     8581 ctggccatgt tggacatggc cgcgggtctc cacgacctcg aaccattcca ggttctggat
     8641 gctcttgctg cactcggcca gcgcgttgtt gattgcatcc tcgatgctga tgcgtgagga
     8701 accgacgatc tcgaccttct tgtagacatg atgatcggac atggcacacc tccttcggga
     8761 tggatctgct cggtcgcgga accagtatgc cagatcggac gcggccaacc catgcggcac
     8821 ggggggcggc tgcggcgtac gacgtttgcc ttgctgcggt tcaggcggcg ccggcgcgcg
     8881 tggcgccgct gttgccgttg cgcaggtcat cgaccagcac gttgaatgcc gcgaccgggt
     8941 cgtgtccggc gtggtgcagc cgcagcaggg cggcgaagcc catgccgtgc atcaggtcca
     9001 tcagcatttg cgccacgttg ttggcgtcga tcgcgggccg cacctcctgc tggtgctgca
     9061 gttgcacgat cacggaggcc agcagcgcgc ggttgcgctc catgtcgttg cgcagcaccg
     9121 ccgctgcccc ggcatccgac gccgcgtggc tccacaactg cagtcgcacg ccccactcga
     9181 tgcgcgattg ccggtcgtgt ggcagcgcgg cctcgatcag cggcagcagg ttgtcgatgc
     9241 tcacaccgcg ggacagcgcg cgcgccaggc gctgctcgat gcgcgcgctg gcccacgcga
     9301 aggccgccgt gacgatgccg gccttgctct cgaagtagtg cgacagcacg ccgatcgagc
     9361 agcccatggc gcgcgcgatg ctgcgcgtgg taagggccgc catgccgtcg gcggcgatga
     9421 ggcgggccgc cacctcggcg atttcccggc gtctctggtc gtggtcgagc ttgcgcacgg
     9481 cgctgagttc tccggctctg gcgcgacgtt tgtttgcatt gcgtcgcaac gggtgttcgc
     9541 atagattata tacatatcaa ccgtccggca tgtttgccgg atgcgcactg gcatcagcgg
     9601 agcaccggcg ccatgaaagc ggcaagtctc gaccacgatt acgtgcaccg gcccgatcac
     9661 aaggatctct cgcacctgcc cggcgcctac ggcctgccgt atctgggcat gatggtgccc
     9721 ctgctgaaga atccctacga ggtgatcaag cgccactacg accgctacgg cgcggtctcg
     9781 cgcacgcggc tcacggggca gcacatggtg tgggcgctgg gcccggacta caaccgcgag
     9841 ctggtgatgg atccgcagca ggtctattcc gcgcgcatgg gctacgacgc gccgctcggg
     9901 gacttcttcg ccggcggcct gctggtgcgc gatttcg
//
LOCUS       DAORUQ010000119         9901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7255_length_9901_cov_5.782145, whole genome shotgun sequence.
ACCESSION   DAORUQ010000119 DAORUQ010000000
VERSION     DAORUQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_9901_cov_5.782145"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLW13_10850"
     CDS             <1..435
                     /locus_tag="PLW13_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQY70917.1"
                     /translation="PLAKAIELVLAAHRNRCRSQRGVGLQLETSPGNGVLDIVVKPLI
                     SDRLLESRDRPAAVVYINDCRQPGIELDPAVLVGMYGFTNCESQLAALLARGTNLSDA
                     AGHLGVSINTVKTHLRGVYEKMGTNKQARVVAQLNHSTARLL"
     gene            complement(445..1677)
                     /locus_tag="PLW13_10855"
     CDS             complement(445..1677)
                     /locus_tag="PLW13_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY70918.1"
                     /translation="MNQRSASGFLGWRMVALAFLAQNLAIGLTFGSFGVLIKPVASEL
                     HASRAMASLGIAVIMLLMGLSGPLLGVLLARYPIRRVMSAGAVLMAGGFALAASAQSF
                     PVFLLGFSVIGGAGCAALGVIPATTLASNWFVARAGLAIGLVSIPLFIAITPPLVAWI
                     VDAYGWRAALYALAAGFVAVLPLLQLVVSRPEDVGQHALGADVAAPTGRADVPPEAPR
                     QLSAFVRSAWYWGALLSAGLLSCGGIVLMSHLVPFATDRGISTTSAALLLSVNGVFSM
                     AGALLFGQVADRIGARATLGLIGLVQAACWSGIIFADSFLLFLPFLVGIGLCGGGTHT
                     AFSALLNAAYGRASFPNALGLATLLMLPFTFVAAPLAGWIFDSTGSYVIAFQVQIAAF
                     LAAAVVYLVIYRGRPAPA"
     gene            complement(1687..2160)
                     /locus_tag="PLW13_10860"
     CDS             complement(1687..2160)
                     /locus_tag="PLW13_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70919.1"
                     /translation="MNTSDAVQQLLDLEAIRTLKHRYIRCMTQSRWDELETLLAPDVE
                     ASYSDGKYVFSDRATLMGFLRQQDPTGVPQLSYWHVTMPEITLHAPDRASGIWAMYHF
                     HLQKKEQQQLEMFAYYSDEYRKLDGRWLIARTGYERVMEQSLDRSTLPALQLLVG"
     gene            2367..3236
                     /locus_tag="PLW13_10865"
     CDS             2367..3236
                     /locus_tag="PLW13_10865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70920.1"
                     /translation="MQFGMSIAFTDPADYVPLAVAAEENGFAAITLPDHLIYPQQLSV
                     PYPYTEDGVPRFVPDDPFPDPWVSAVAMATATKTLWFYTSVFVLPARNPVHVAKILAS
                     AACLSGNRIALGAGMGWMPEEFRIGEQPFEQRGKRADEMIEVMRKLWSGEWVEHHGEF
                     YDFAPVKMRPVPATPIPIYVGGFSKPALRRAARNDGWISDLHTLKELEALIREVRAYR
                     AEAGKANEPFRILGFSCSDAWGPDGFRAMRDIGLDVVSTMPGAFYGVDMKAPVARKID
                     AIKRFADDVIAKI"
     gene            3277..4071
                     /locus_tag="PLW13_10870"
     CDS             3277..4071
                     /locus_tag="PLW13_10870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70921.1"
                     /translation="MRIDLRGLTALVTGSTRGIGFAVARGLAECGASVIVNGRREEAV
                     AQAVADLSQRVPGASFLPACADVGRAEGVGRLFAEYPRVDILVSNAATFDWTPFFDTD
                     DADWQRHFDINVMAGVRLARRYLPWMLEQNWGRVVLVASEAGLNIPADMIHYGVSKAA
                     EIALARGLAELTAGTGVTVNSVLPGPTASSDAVRFLDDYARDASVPRSDAERHFVASA
                     RPSSLLGRMATVEEVANMIVYASSPLASATNGAALRVEGGILRHPG"
     gene            4227..5156
                     /locus_tag="PLW13_10875"
     CDS             4227..5156
                     /locus_tag="PLW13_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQY70922.1"
                     /translation="MTGKRQQAPRNAALAYETNNYSPRLAPFYEPAAGFWPPLAEQLE
                     AAAQAGFGCVVLDSGSLETCMRQGGSLPGLRARLDVLALHCLAVAEISIVEDTESVIG
                     AARPLLDAVRALGAPFLQASVMGVAGNCLASARALEQLAAAAGATVALEFLPFGAVNS
                     IAAAQAFVRAAELCNTRLLLDSWHFFQGPSSWEQLRGLWPQQIAYLQFSDHAAIGVEH
                     ALHATVNERLLPGEGVFDLARFCAELRSIGFDGVLSCEVLSSAWRERTPAQYALAEFA
                     AARRYWGDDAGNRGRGLHRALIADRGTATDDNP"
     gene            5170..5667
                     /locus_tag="PLW13_10880"
     CDS             5170..5667
                     /locus_tag="PLW13_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70923.1"
                     /translation="MDPVQFLLDAHQIGQCLLKYPVALDSRDFDLLESLFTPDARIDI
                     PAAGVFDPGGYRAASAAGLGRLDATQHFVGQPLLKIEGDRAWARSYLLAQHAVNALAP
                     AGTLTIGAWYNDELVRREGSWLIAARTGNAVWWSGNPAVLGLEGVPNAFPRHEGHLSP
                     AWLGS"
     gene            5767..6741
                     /locus_tag="PLW13_10885"
     CDS             5767..6741
                     /locus_tag="PLW13_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQY70924.1"
                     /translation="MDQSIPGQGPRCPTDPDFTDALYHRVAIARAMEICMERHTREHR
                     FSGWWHPGEGQEAAGVGATAAMRQDDYLFYQGRGCAWAIGKGMAPDPIFGDLFGRITG
                     ATRGKGGGVPHWADYSIGVMGEGATLGSVYPLAAGAALAARLRRTDQVALANFGDGTA
                     TRGTFHETLVHAAAWKLPLIYFCENNGLLVGTRLASVSPTENIADYAAGYGIPGVIVD
                     GQDALAVFEATTAAIARARAGDGPTLIEAKVFRRRGHYAGDPQHYRAPDYASAYRDPL
                     ALLEARLAPDRALALRSAAQAQVQTAYEAALAAPGADSSVIYRDLYHG"
     gene            6734..7726
                     /locus_tag="PLW13_10890"
     CDS             6734..7726
                     /locus_tag="PLW13_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQY70925.1"
                     /translation="MAEHGTAVMAYNAAMGLALIEEMRRDERVFVLGQGVATGGWFGA
                     EKGLADEFGRERVIDSNIAEAFEAGVAAGAAIVGMKPVINMGFGDFALIAGDEIFHKL
                     AKWRYMHGLDVPMTAVVIFPIGVMGGAGPEHSSCPEVLGMHFPGLKVVVPATPADAKG
                     LMAAALREPNPVLFHSVNSLGFQRGEVPLDPDFIVPIGKGVVRRAGNAITAITYGSMV
                     DRTLAAADRVADEGIDVEVIDLRSLVPLDWELVLSSVRRTHRAMVVHEAVKTAGPGAE
                     IAAQIQERLFFELEAPILRVAAKDVPLPQNGDLEQLCIPSVDEIADAARRLIAI"
     gene            7738..8997
                     /locus_tag="PLW13_10895"
     CDS             7738..8997
                     /locus_tag="PLW13_10895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQY70926.1"
                     /translation="MVSLGELRIPHMGSVENARVVAWHVEEGAGFSVGQLLYEVETDK
                     TLTEVTADVDGVLARRLAHEGDELKVGELVGWWAAAGSDAGVIAAALAAQPAAPQMAA
                     ADTAFTAVSRASAAAPAAPICSADEASQHSPRVRKLAREHGVDLATLRGSGPGGRISG
                     EDVLQAVAARSGGRGSEEQGDPLPAGYEDVPCESRPNSLRRRTIARRLAETARSVPHL
                     TADMQIDMGAVLASRARLNARQPAAPMSVLSLVAAAVCRLLPQHPELNATFTDAHLLC
                     WRVVNLGIAVDTPEGLVVPVLRDAGRMSLEELNGAIATLAARARNGTLRANELEGGTF
                     TISNPGSLGPVLRAEAILNAPQVALLGLPGMVHEPVAVADATNGYRIAVRPLLRPSLT
                     FDHRALDGGHVIRFLNALKRELEQMSG"
     gene            complement(9031..>9901)
                     /locus_tag="PLW13_10900"
     CDS             complement(9031..>9901)
                     /locus_tag="PLW13_10900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY70927.1"
                     /translation="MKFWQMLPWMEPDQIIAAARFAEDVGFHGVMLADHGIYPLELKA
                     PYPYSADGRPPMGSDAWYPDVWATLGAISASTTRLHMSVAVYVLALRNPFEVARAIGT
                     LDILSGGRVALGAGTGWMKDEYDLYGVDFATRGQRMDEMIEILRGFWREGIVEYHGKH
                     FDFPRVEISPAPKRNVPIYIGGAAPVALRRAARCEGWIGAGNAPAEVPALLAQIRDYR
                     AGLGLTMDGFETVIGLTTPFDPALLASLEEQGMTAGVNPPFRFLLGERSSLDAKKRNM
                     EDFAKRFIVNQRA"
BASE COUNT         1598 a         3229 c         3504 g         1570 t
ORIGIN      
        1 ccgctcgcca aggccatcga gctcgtgctc gcggcgcacc gcaatcgttg ccgctcgcag
       61 cgcggcgtcg ggctgcaact cgagaccagc ccgggcaacg gcgtgctcga catcgtggtc
      121 aagccgctga tcagcgatcg cctgctggag tcgcgcgaca ggccggccgc ggtggtctac
      181 atcaacgatt gccgccagcc gggcatcgag ctcgatccgg cggtgctggt ggggatgtac
      241 gggttcacca actgcgagag ccagctcgcg gcgctgctcg cgcgaggcac aaacctgagc
      301 gacgccgccg ggcatctggg cgtcagcatc aacaccgtga agacccacct gcgcggcgtc
      361 tacgagaaga tgggcaccaa caagcaggcc cgggtcgtcg cgcagctcaa tcattccacc
      421 gcgcggctgc tctgaacccg ggcttcaggc cggcgccggg cgcccgcggt agatcaccag
      481 gtagaccacg gccgcggcga ggaacgccgc gatctggacc tggaaggcaa tgacgtaact
      541 gccggtgctg tcgaagatcc agccggcgag cggcgcggcc acgaacgtga agggcagcat
      601 caaaagcgtg gccagaccca gcgcattggg gaaggacgcg cggccgtagg cggcgttcag
      661 cagcgcgctg aaggcggtat gggtgccgcc gccgcacagg ccgatgccga cgaggaacgg
      721 caggaacagc aggaagctgt cggcgaaaat gatcccgctc cagcacgccg cctgcaccag
      781 gccgatcagt cccagtgtcg cgcgcgcgcc gatgcgatcg gcgacctgcc cgaacaacag
      841 cgcaccggcc atggagaaaa ccccgttcac cgacaacagc agggcggccg aggtcgtgga
      901 aatgccgcga tcggtcgcga agggcaccag gtggctcatc agcacgattc cgccgcagct
      961 cagcagcccg gcgctcagca atgcgcccca gtaccaggcg ctgcgcacga aggcggagag
     1021 ttggcgcggc gcttcgggcg gcacatccgc acggccggtt ggcgccgcca cgtcggcgcc
     1081 gagtgcgtgc tggcccacgt cctccggccg gctcacgacc agctggagca gcggcagcac
     1141 ggcgacgaag cccgcggcca gcgcgtagag cgccgcgcgc cagccgtagg cgtccacgat
     1201 ccaggccacc agtggcggtg tgatggcgat gaacagcgga atgctgacca gcccgatcgc
     1261 cagccccgcg cgcgctacga accagttgct ggccagcgtg gtcgcgggga tcacgcccag
     1321 ggcggcgcat ccggcgccac cgatcacgct gaagccgagc aggaacacgg gaaagctctg
     1381 cgcgctggcc gcgagcgcga aaccgccagc catcaatacc gcaccggcgc tcatcacgcg
     1441 ccgtatcggg tagcgcgcca gcaggacgcc gagcagcggg ccggaaagcc ccatcagcag
     1501 catgatcacg gcgatgccca gcgaggccat ggcccgcgag gcatgcagtt cgctggccac
     1561 cggcttgatc agcacgccga agctgccgaa agtcaggccg atggcgagat tctgcgccag
     1621 gaaggccagc gcgaccatgc gccagccgag gaagcctgaa gccgatcgct ggttcattgg
     1681 cgcgcctcag ccgaccagta gttgcagggc gggcagggta ctgcgatcca gcgactgttc
     1741 catgacccgc tcgtaacccg tgcgggcgat gagccagcgt ccgtcgagct tgcggtattc
     1801 gtcgctgtag taggcgaaca tctccagctg ctgctgctct ttcttctgca ggtggaagtg
     1861 gtacatcgcc cagatgccac tcgccctgtc cggcgcatgc agcgtgatct cgggcatggt
     1921 cacatgccag tagctgagct gggggacgcc ggtgggatcc tgctggcgca ggaagcccat
     1981 cagggtcgcg cgatcggaga acacgtattt gccgtccgaa tacgaggctt cgacgtcggg
     2041 ggcgagcaag gtctccagtt cgtcccagcg cgattgcgtc atgcagcgga tgtagcggtg
     2101 cttgagcgtc ctgatggcct cgagatcgag cagttgctgc acggcgtcac tggtgttcat
     2161 ggcaggatcc tttttgtcgt gggtcgaagc atgccgtgcg cgggcgagca tggccaacgc
     2221 ggcgccaggc cgacagtatt cgcccgggcg ggtccgcggg attcatccgc gcggatgaag
     2281 gcgcccggtt catgccatcg ggtgatgcgg gccgggccgc gctggcgcag catcggcact
     2341 gaccatccat cctggagtga atccccatgc aattcggcat gtccatcgcg ttcaccgatc
     2401 ccgcggacta cgtgccgctg gcggtcgcgg ccgaggagaa cggcttcgcc gcgatcacgc
     2461 tgcccgatca cctgatctat ccgcagcagc tctcggtgcc gtacccgtac acggaagacg
     2521 gcgtgccgcg cttcgtgccg gacgatccct tccctgaccc ctgggtgtcg gcggtcgcga
     2581 tggccacggc gacgaagacg ctgtggttct acaccagcgt gttcgtgctg ccggcgcgca
     2641 atcccgtgca cgtcgcgaag atcctcgcga gcgccgcctg cctgagcggc aaccgcatcg
     2701 cgctgggtgc cggcatgggc tggatgcccg aggagttccg catcggcgag cagcccttcg
     2761 agcagcgcgg caagcgcgcc gacgagatga tcgaggtgat gcgcaagctc tggagcggcg
     2821 agtgggtgga gcaccacggc gagttctacg acttcgcgcc ggtgaagatg cgcccggtgc
     2881 ccgcgacgcc gatcccgatc tacgtcggcg gcttctcgaa gcccgcattg cgccgtgccg
     2941 cgcgcaacga cggctggatc tccgacctgc acacgctgaa ggaactggag gcgctgatca
     3001 gggaggtgcg cgcttaccgg gcggaggcgg gcaaggcgaa cgagccgttc aggatcctcg
     3061 gcttcagttg cagcgacgcc tgggggccgg acggttttcg cgcgatgcgc gacatcggcc
     3121 tcgatgtggt gtccaccatg cccggcgcgt tctacggcgt cgacatgaag gcgccggtgg
     3181 cgcggaagat cgatgcgatc aagcgcttcg cggacgacgt gatcgcgaag atctgaaacg
     3241 ggcgtcttgg cgccaccggc gtaacaggga caatccatgc gtatcgattt gcgcggcctc
     3301 accgcgctgg tcaccggatc gacccgcggg atcggctttg cggtcgcccg gggccttgcc
     3361 gaatgtggcg cgagcgtgat cgtcaacggc cgccgcgagg aagcggtcgc gcaggccgtg
     3421 gccgatttgt cgcagcgcgt gccgggagcg tcgttcctcc cggcgtgcgc cgatgtcggc
     3481 cgcgcagaag gtgttggccg gttgtttgcc gagtatccgc gagtcgatat cctggtttcc
     3541 aacgccgcga cattcgactg gacccccttc tttgatacgg acgacgccga ctggcaacgg
     3601 catttcgata tcaatgtgat ggctggggtt cgcctggcgc gacgctacct gccgtggatg
     3661 ctggagcaga actgggggcg cgtggtcctg gtcgcgtccg aggcgggcct gaatattccg
     3721 gcggacatga tccattacgg cgtcagcaag gccgccgaga tcgcgctcgc ccgggggctc
     3781 gcggagctca ccgcggggac cggtgtcacg gtcaacagcg tgcttccggg gccgacggcc
     3841 tcgagcgatg ctgtccggtt tctggacgac tatgcccgcg acgcaagcgt gccgcgcagc
     3901 gatgccgaac gtcatttcgt cgcctcggcg cggccgagct cgctgcttgg acgcatggca
     3961 acggtggagg aagtggcgaa catgatcgtg tatgccagct cgccactggc ctcggcgacc
     4021 aatggcgccg cgctgcgcgt ggaggggggg atactgcgac atccggggtg atgcgcggtt
     4081 cgttcccgat cccggcccgg ctggcgcgcc tcgccgcctg cttcattcga acgcgcgaag
     4141 cgcggcgggc gggtcacgcc ttaggctgcg cacaacgagc acggaggagt caggatgttc
     4201 ccgccagcgc catgcgtgga caccgcgtga cgggcaagcg ccagcaggcg ccgcgcaatg
     4261 ccgcgctcgc ctacgaaacg aataattatt cgccgcgcct ggcgccattc tacgaaccgg
     4321 cggcagggtt ctggccgccg ctggccgagc aactcgaggc cgcggcgcaa gcgggcttcg
     4381 ggtgcgtcgt gctcgacagc ggctcactcg agacctgtat gcgccagggc ggctcgctgc
     4441 ccggtttgcg cgcgcggctc gatgtgctgg cgctgcattg cctcgcggtg gcggagatca
     4501 gcatcgtgga ggacaccgag tccgtgatcg gcgcggcgcg gccgctgctc gatgcggttc
     4561 gagccctcgg ggcgccgttc ctgcaagcga gcgtgatggg cgttgcgggc aattgcctgg
     4621 cgtcggcgcg cgcgctggag cagcttgccg ccgcggccgg tgcgacggta gcgctggagt
     4681 tcctgccctt cggcgccgtc aacagcatcg ccgcggcgca ggcatttgtg cgggccgcgg
     4741 agctgtgcaa tacccgtctg ctgctcgaca gctggcattt cttccagggc ccgagcagct
     4801 gggagcaact gcgcgggctg tggccgcagc agatcgcgta cctgcagttc tccgatcatg
     4861 ccgcaatcgg ggtggagcac gcgctgcatg cgacggtcaa tgagcgcctg ctgcccggtg
     4921 agggtgtgtt cgacctggcg cgattctgcg cggagctgcg cagcataggt ttcgacggtg
     4981 tgctgagctg cgaggtactg tcgagcgcgt ggcgcgagcg cacgcccgca cagtatgcgc
     5041 tcgcggaatt cgcggctgct cgccgctatt ggggggatga cgccggcaac cggggtcggg
     5101 gcctgcaccg ggccctgatc gcggaccggg ggacggcaac ggatgacaac ccatgacgag
     5161 gagccgacga tggaccctgt gcaattcctg ctcgacgcgc accagatcgg gcaatgcctg
     5221 ctgaagtacc cggtggcgct ggattcgcgc gacttcgatc tgctcgagtc gctgttcacc
     5281 ccggatgcac gcatcgacat tccggccgcc ggggtgttcg accccggcgg ctatcgcgcg
     5341 gcgtcggcgg cgggactggg gcgcctcgat gccacgcagc atttcgtggg ccagccgctg
     5401 ctgaagatcg agggcgatcg cgcctgggca cgcagctacc tgctcgcgca gcatgcggtg
     5461 aatgcgctgg cgcccgccgg caccctgacg atcggcgcgt ggtacaacga tgaactcgtg
     5521 cgccgcgagg gctcgtggct gattgccgcg cgcaccggca acgcggtgtg gtggagcggg
     5581 aatcccgcgg tacttggtct ggagggagta ccgaatgcgt ttccccggca cgaggggcac
     5641 ctcagccccg cgtggctggg cagttagcac tgcatatttc tcctgcgatt tcccccgttt
     5701 gcatgaggtc gcgacagtcg cttcgcgcga aagtgaagct cgacagtggc acggggagcg
     5761 cggcaaatgg atcaatcgat acccgggcaa gggccacgct gccccacgga tcccgacttc
     5821 accgatgcgc tgtaccatcg ggtggcgatt gcccgtgcga tggaaatttg catggagcga
     5881 cacacccggg aacatcggtt ctccgggtgg tggcatcctg gcgaggggca ggaggcggcg
     5941 ggcgtcggcg cgactgccgc gatgcggcaa gatgactacc tcttctacca gggtcgcggc
     6001 tgcgcctggg caatcggcaa gggcatggcg ccggacccga tcttcggtga cctgttcggc
     6061 aggatcacgg gcgcgacgcg cggcaagggc ggcggtgttc cgcactgggc cgattactcg
     6121 ataggcgtga tgggcgaagg ggcgacactc ggcagcgtgt acccgcttgc ggccggtgcc
     6181 gcactggcgg cgcgactgcg gcgtacggac caggtggcac tcgcgaactt cggcgacggc
     6241 accgcgaccc gcggcacctt tcacgaaacg ctggttcacg ccgcggcatg gaaactgccg
     6301 ctgatctact tctgtgagaa caacggcctg ctcgtcggca ctcgcctcgc gtccgtcagc
     6361 cccaccgaga acatcgccga ttacgccgcg ggctacggca tccccggtgt gatcgtcgac
     6421 ggacaggatg cgctggcggt attcgaggca accaccgccg cgatcgccag ggcgcgcgcg
     6481 ggcgacgggc caaccctgat cgaggccaag gtctttcgcc gtcgcggcca ctacgcgggc
     6541 gacccgcagc actaccgcgc gccggactac gccagcgcat accgcgatcc gctcgcgctg
     6601 ctcgaggcgc gcctcgcgcc ggatcgagcg ctcgccctgc gcagtgcagc gcaggcgcag
     6661 gtgcagacgg cctacgaagc cgcgctcgcg gcgccgggcg cagactcatc tgttatttat
     6721 cgggaccttt accatggctg aacacggcac ggcagtgatg gcctacaacg cggcgatggg
     6781 cctggcgctg atcgaggaaa tgcgtcgcga cgaacgagtc ttcgtactcg ggcagggggt
     6841 tgcgaccggc ggatggttcg gcgccgagaa gggactggcc gacgagtttg gccgcgagcg
     6901 cgtgatcgac agcaatatcg cggaagcgtt cgaggcaggt gtcgccgccg gtgcggcgat
     6961 cgtgggcatg aagcccgtga tcaacatggg tttcggcgat tttgcgctga tcgccggcga
     7021 cgagatcttc cacaagctcg ccaagtggcg ctacatgcac gggctcgatg ttccgatgac
     7081 agccgtggtg atctttccta tcggcgtgat gggcggcgcg ggccccgagc actccagttg
     7141 ccccgaggtg ctgggcatgc actttcccgg actgaaggtc gtggttccgg cgactcccgc
     7201 cgatgccaag ggtttgatgg cggcggcatt gcgcgagccc aacccggtgc tgttccacag
     7261 cgtcaacagc cttggcttcc agcgcgggga agtgcccctg gatccggatt tcatcgtgcc
     7321 gatcggcaag ggcgtggtgc ggcgagcagg caacgccatt accgcgatca cctacggctc
     7381 gatggtcgat cgaacactcg cggccgccga tcgggtcgcc gatgagggca tcgacgtcga
     7441 ggtcatcgac ctgcgctcgc tggtaccgct cgactgggag ctggtgctgt cctcggtgcg
     7501 gcgcacacac cgcgcgatgg tggttcacga ggccgtgaag accgcggggc ccggcgcgga
     7561 gatcgccgca cagatccagg aacgcttgtt cttcgaactc gaggcgccga tcctgcgcgt
     7621 ggcagccaag gacgtgccgc tgccgcagaa cggggatctg gagcagctgt gcatcccgtc
     7681 ggtcgacgag atcgccgacg cggcgcgccg gctcattgct atctgagagc agagatcatg
     7741 gtcagcttgg gcgaattgcg gatcccgcac atgggctcgg tcgagaacgc gcgcgtcgtc
     7801 gcctggcacg tagaggaggg ggccggcttt tccgtcggcc agctgctcta cgaagtagag
     7861 accgacaaga cgctgaccga ggtgaccgcc gacgtcgatg gtgtactggc gcgccgcctg
     7921 gcacacgagg gcgacgagct gaaagtcggt gagctcgtgg gctggtgggc tgcggcgggt
     7981 tcggatgccg gggtgatcgc cgcggccctt gcggcgcagc ccgctgcgcc gcagatggcg
     8041 gcggccgaca cggcgttcac cgcggtgtcg cgcgcttcgg ccgcggctcc cgcggcgccg
     8101 atttgcagtg cggatgaggc ctcgcaacat tcgccgcgag tgcgcaagct tgcgcgcgag
     8161 cacggtgtgg acctcgccac gctgcgcggc agcggcccgg gcggacggat cagcggcgag
     8221 gacgttctgc aggccgtcgc agcgcgctct ggcgggcgcg ggtcggagga gcagggtgac
     8281 ccgctgccag cgggctacga ggacgtcccc tgcgagtcgc ggccgaacag cctgcggcgg
     8341 cgcaccatcg cgcggcgtct ggcggaaacc gcgcgctcgg tgccgcatct cacggcggac
     8401 atgcagatcg atatgggcgc tgtgctggcc tctcgcgcgc gcctcaatgc ccgtcagccg
     8461 gcggcaccga tgtcggtgct gagcctcgtc gcagcggcgg tatgccggct gctcccacaa
     8521 cacccggagc tgaacgcgac gttcacggac gcgcatttgc tgtgctggcg cgtggtgaat
     8581 ctcggtatcg ccgtcgacac ccccgagggg ctcgtggtgc ccgtgctcag ggatgcaggg
     8641 cggatgtcgc tcgaggaatt gaacggcgcg atcgccacgc tcgccgcacg tgcccgcaac
     8701 ggcacgctgc gcgccaacga actggaggga ggcacgttca cgatttccaa cccgggatca
     8761 ctcgggccgg tgctgcgcgc cgaggcgata ctgaacgcgc cacaggtcgc gctgctcgga
     8821 cttcccggca tggtgcatga gccggtcgcg gtggccgatg cgacaaacgg ttaccggatc
     8881 gcggtgcggc cgctgcttcg gccctcgctg acattcgatc atcgcgcgct cgacggaggg
     8941 cacgtgatcc gctttctgaa tgcattgaaa cgcgagctgg agcaaatgtc cgggtaacgc
     9001 tccactgcgc ttcgatcgcc gcttcctgcg ctaggcacgc tggttaacga tgaagcgctt
     9061 cgcgaagtcc tccatgttgc gcttcttcgc atcgagcgag gagcgctcgc ccagcaagaa
     9121 gcgaaacggc gggttcaccc cggccgtcat gccctgttcc tcgaggctcg cgagcagcgc
     9181 gggatcgaat ggcgtggtga gcccgatcac ggtctcgaag ccgtccatcg tcaagccgag
     9241 cccggcgcgg tagtcgcgga tctgcgccag cagcgcgggc acttccgcgg gcgcgttgcc
     9301 ggcaccgatc cagccctcgc agcgcgcagc gcggcgcagc gcgacgggtg cggcgccgcc
     9361 gatatagatc ggcacgttgc gtttcggggc gggggagatt tccacgcgcg gaaaatcgaa
     9421 gtgcttgccg tggtattcca cgatgccctc gcgccagaag ccgcgcagga tctcgatcat
     9481 ctcgtccatg cgctgcccgc gtgtcgcgaa atcgacgccg tacagatcgt actcgtcctt
     9541 catccagccg gtgccggcgc cgagtgccac gcgcccgccg ctcaggatgt ccagggtgcc
     9601 gatggcccgc gcgacctcga agggattgcg cagcgccagc acgtagaccg ctaccgacat
     9661 gtgcaggcgc gtggtcgacg ccgagatcgc gccgagcgtg gcccacacgt cgggatacca
     9721 ggcgtcggag cccatcggcg gcctgccgtc ggcggaatag gggtagggcg ccttcagttc
     9781 gagcggatag atgccgtgat cggcgagcat cacgccgtgg aatcccacgt cctcggcgaa
     9841 tcgcgccgcc gcgattatct gatcgggttc catccacggc agcatttgcc agaatttcat
     9901 c
//
LOCUS       DAORUQ010000120         9778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7385_length_9778_cov_6.335082, whole genome shotgun sequence.
ACCESSION   DAORUQ010000120 DAORUQ010000000
VERSION     DAORUQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_9778_cov_6.335082"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLW13_10905"
     CDS             complement(<1..358)
                     /locus_tag="PLW13_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70928.1"
                     /translation="MAARKIVVVGNGMVGHHYVEQLLASGLELDITVLGAEPRPAYDR
                     VHLSEFFAGRKPEELALTTREHYRAQDVKAHFGDAVTAIDRDAKVVATAAGRRFAYDT
                     LVLATGSYPFVPPIPGG"
     gene            complement(662..1891)
                     /locus_tag="PLW13_10910"
     CDS             complement(662..1891)
                     /locus_tag="PLW13_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY70929.1"
                     /translation="MARERLLIVGSGMAAARLLEELAARGCADEITVVGEEPQPSYNR
                     ILLSSLLCGEKQPADLPLLDRDWYRAHGVLLLTGEAVTRVDAGERVAWTSRARRIRFD
                     RLVFATGARGQLPVIPGVASERVLAFRTLQDLAALRRLAVPGEPAVVVGGGLLGLEAA
                     HGLDALGLQVTVVHRQPYPMNRQLDPQAGALLQQQLQRRGIRFALGASPVAVGSLQGA
                     VASVRLDDGRSLPARLVVFAAGIAPNVEVAAAAGVPCARAIRVDRWLRTGMPGIHALG
                     ECCELEGRGFGLVAPVWRQARVLAEVLTGQAGAGFAHVEDPVQLKVSGVELFSAGAMP
                     FAADAESQVLQDTARGIYRRLVFAGGRLVGAILLGDRRGGSWYGELIAAGQDVSALRP
                     RLMFGREFCAADVAVAA"
     gene            complement(1891..3240)
                     /locus_tag="PLW13_10915"
     CDS             complement(1891..3240)
                     /locus_tag="PLW13_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQY70930.1"
                     /translation="MMNRTMRRGAHHLLALAVIAASAAQAEADPLTDALMKSKVSGNF
                     RVRQESVDLDSATVDDASALTLRSRLGIETAPQKGLTAILEFEDTRALFGQDEYAPET
                     AGYAVVVDPENTEVNRAYLRYRGISRLDMGLGRQRLVLDNQRFVGNVGWRQDEQTFDA
                     FSASYTGVPDWSLFYAYVDHVNGIADVEPTFNFDIDSSDHLLNAAWTGLKFGKLSAYC
                     YALENEEPDAALRNLPPRVNALNPTLRYRENATAGLRFDGNYTLPVTLPVRLLYTAEY
                     AIQELENATGLDFDTDYSLIDAGAGYVTGIGVVSARLARETLGSDRAGNAMQGFQTPY
                     ATKHAFNGWVDMFLNTPAGGLVDTYLTLAADLTPYGIKLMAVYHEYAEDDGPLRGGAP
                     RDFGSEWNLQGTRQFGAHYTVGLKYGNYEADGDVPVLIGTTANVDTEKFWLWAEYNF"
     gene            complement(3278..4255)
                     /locus_tag="PLW13_10920"
     CDS             complement(3278..4255)
                     /locus_tag="PLW13_10920"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY70931.1"
                     /translation="MPRRREINSPGDRTVKTLLELSQVGIEFKTDKGPFKALDNVNLK
                     IREGEYVSLIGHSGCGKSTVLNIVAGLLRATTGGVVLDGREVTEPGPERAVVFQNHSL
                     LPWLSAYQNVELAVRQVFRAKKSRAEMREWIEHNLKLVHMEHALHKRPGEISGGMKQR
                     VGIARALAMQPRVLLMDEPFGALDALTRAHLQDSLMEIQRELANTVIMITHDVDEAVL
                     LSDRIVMMTNGPAATIGEILDVDLERPRNRLELAEDPRYNHCRQEVLRFLYEKQRKVD
                     GTARASPGASQPEPAHAPLAAKAPAGAQMPQPARGERAVVTPLHGKHVA"
     gene            complement(4255..5259)
                     /locus_tag="PLW13_10925"
     CDS             complement(4255..5259)
                     /locus_tag="PLW13_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY70932.1"
                     /translation="METTRTPAWFAARARAWDARGAGRQLASLTRALAMPLLGIAIFL
                     LLWQAGAARVQTTLGQLPGPALVWSEAKGLHREYLMDREREAAFHARQQERAAKMAAA
                     NPGAAAAPVRPYTASPTFYTQIVTSLKTVMSGFLLSALLAIPVGIAIGLSGPLHGAVN
                     PLIQVFKPVSPLAWLPLVTLVVASVHDNPDPAVPKSFITSLITVALCCVWPTVINTAV
                     GVASVSDDLNNVSKVLRLGWLQHVRRIVLPAAVPMIFTGLRLSLGVAWMVLIAAEMLS
                     QNPGLGKFIWDEFQNGSSQSLGRIMVAVITIGLIGFLLDRVMLALQRVVSWDKNAVTR
                     "
     gene            complement(5306..6700)
                     /locus_tag="PLW13_10930"
     CDS             complement(5306..6700)
                     /locus_tag="PLW13_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY70933.1"
                     /translation="MTGRHEGRTRGWRARVAGILGLCAAGLLAGAPASAAIGEPEKWE
                     LKFGFIKLTDMVPLAIAYERGYFEDEGLYVTLEAQANWKVLFDRVVSGELDGAHMLAP
                     MPLGAATGVITKSEIVTPFTLSYNGAAITVSNAVWAEMEKTVPVEDGKPKHPVSAAAL
                     KPVLESYKAQGRTFNLAMTFPVGTHNYHLRYWLAAGGINPGVYAPQKGDNSGSLGAEA
                     LLSVVPPPQMVPTMEAGTTAGYCVGEPWNQQAVTKGLGVPVITSENIWTDGADKVFGM
                     TGEFVQKNPNTTVRVVKALIRASYWLDENGNANRPEAVKIIARPAYVGADEKVIANSM
                     TGTFEFEKGDKRPVPDFNKFFRGQYGIPYYSDAIWWLTQMRRWGHITEHQPDSWYLET
                     AKRAFRPDIYVAAAKELIADGVMQASDFPDLDKATFIKAPQAGPLDGIVYDGTQPNAY
                     IDSFAIGLKGSAQP"
     gene            complement(6973..8175)
                     /locus_tag="PLW13_10935"
     CDS             complement(6973..8175)
                     /locus_tag="PLW13_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY70934.1"
                     /translation="MSRDKQLPPPEKSALTLGFMRLSDSAPLVMARETGLFARYGLEV
                     ALAREVSWANLRDKLVVGKLDAAHLLAPLPMMTGLGAGGMRANLLTGLSLGLNGNAIT
                     LSRALWEALGGERDASAPGMAAALHERIAGSPGAGPLTLATVHLFSMHTFQLRMWLRA
                     GGVDPDREVRIIVLPPEQMCDSLARGIIDGFCVGEPWNTLAVQQGIGAVAATGHEIWN
                     NAPEKVLGVTEAWHNHHPATHLRLRIAIMAACQALAEPAQRELAADILSQAQYLGLPA
                     KVLLPALTGRFCRAKHLAPMDAPDFHVFGRYCAGFPWRSQAQWMVRSSGALLGKDIAE
                     EQCRALVQQSWRPDLYREAARHLGLPAPSRDARPEGLHAGCWEFEPGVVLGPDLMIGG
                     GQFRLSAG"
     gene            complement(8172..8792)
                     /locus_tag="PLW13_10940"
     CDS             complement(8172..8792)
                     /locus_tag="PLW13_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HQY70935.1"
                     /translation="MNTDSSKPPARVMLVDDSPERAQLVEERLWAAGFEVVAVIASAK
                     GLLFQIQQHAPDVILIDIESPDRDVLESLAVVNHHNPRPVVMFTQQDDPDYIEQAVGA
                     GISTYLVGGINPEQVKPVIDVAIAQFRAFQALREELHDTRIQLEDRKLIEQAKGLLMA
                     YQKLSEDGAHRMLTRLAMDANQRLPDVARTVLATLSPGGKGKGRSR"
     gene            9068..9472
                     /locus_tag="PLW13_10945"
     CDS             9068..9472
                     /locus_tag="PLW13_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQY70936.1"
                     /translation="MSTYSIGELARRAGVAIDTVRYYERNQLLDPAGRLASGYRRYGD
                     SELRRLRFIRRAKALGFSLADVRALLSLSDERNVAKVKRAAEARLAEVEQRLAELERI
                     RNALQALVAACPGHGRAEACPILNALNQEDAP"
     gene            9469..>9778
                     /locus_tag="PLW13_10950"
     CDS             9469..>9778
                     /locus_tag="PLW13_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HQY70937.1"
                     /translation="MNTLHSPAPSPADASAHCSHGHAAATFIDPVCGMTVKPESPHHA
                     VHAGREYRFCSAGCRGKFVADPARWLEPRDPEPATALPGATYTCPMHPEIIRDHPSSC
                     P"
BASE COUNT         1481 a         3489 c         3235 g         1573 t
ORIGIN      
        1 caccccccgg aatcggcggc acgaaggggt acgatccggt cgccagcacc agggtgtcgt
       61 aggcgaagcg gcgccctgcg gcggtggcca cgactttcgc gtcgcgatct atcgccgtca
      121 cggcatcccc gaaatgcgcc ttcacgtcct gcgcgcggta atgctcgcgc gtggtgagcg
      181 ccagttcctc gggcttcctg ccggcaaaga attccgagag gtgcacgcgg tcgtaggccg
      241 gacgcggctc ggcgcccagc accgtgatgt ccagttcgag cccgctcgcg agcaattgct
      301 ccacgtagtg gtgacccacc atgccgttgc ccaccaccac gatcttcctg gctgccatcg
      361 aagctctccc tcgtcgttgc ccgcgcggtg cggcgaacgg caacgctccc tgccgccagt
      421 ggcggcaagg gcgtcgttac ccggggagag ttgcagctga ccggcggcgc cgtcggcggc
      481 gtccgcgcga tgttgttgaa ccagcgtcgc acgctgtctg ctcgatcaat acaaacgccg
      541 tgccatccct gcgatccacc gcctttggcg gcgaatcacg gcgttttcac tcccttcgcg
      601 gcgcagaggc gcaccaaagc ggtgagcgca gggcgccgtg acagcaagaa tggtgcgcaa
      661 gctacgccgc gactgccaca tccgccgcgc agaattcgcg cccgaacatc agccgcggac
      721 gcagtgccga gacgtcctgc cccgcggcga tcagttcgcc gtaccagctg ccgccgcgcc
      781 ggtcacccag caggatcgcg ccgaccagtc gcccgcccgc gaacaccagc cggcggtaga
      841 tcccgcgcgc cgtgtcctgc agcacctggc tctcggcatc cgccgcaaac ggcatcgcgc
      901 cggcagagaa cagctcgacg cccgagacct tgagttgcac cggatcctcc acgtgcgcga
      961 agcccgcgcc ggcttgcccc gtcagcacct cggctagcac ccgcgcctgg cgccacaccg
     1021 gcgccaccag gccgaagccg cggccctcga gctcgcagca ttcaccgagg gcgtggattc
     1081 ccggcatgcc cgtgcgcaac cagcggtcga cgcggatcgc gcgcgcgcag ggcacaccgg
     1141 ccgcggcggc cacctcgacg ttgggcgcga tgcccgcggc gaacaccacg agccgcgccg
     1201 gcagcgagcg tccgtcgtcc aggcgcacgc tcgcgaccgc cccctgcagg ctcccgaccg
     1261 ccaccgggct cgcgcccagc gcgaagcgga tcccgcgccg ttgcagttgc tgctgcagca
     1321 gggcgccggc ctgcggatcg agctggcggt tcatcgggta gggctggcgg tgcaccaccg
     1381 tcacctgcag cccgagcgca tccaggccat gggccgcctc gagcccgagc aggccgccgc
     1441 cgacgaccac cgccggctcg ccgggcacgg ccaggcggcg cagcgccgcc aggtcctgca
     1501 gggtgcggaa tgccagcacc cgctccgagg ccacgccggg aatcaccggc agctggccgc
     1561 gggcgccggt ggcgaacacg aggcgatcga agcggatgcg gcgcgcgcgc gaggtccacg
     1621 cgacccgctc gcccgcatcc acccgcgtca cggcttcgcc cgtcagcagc agcacgccgt
     1681 gcgcgcggta ccagtcgcga tcgagcagcg gcaggtccgc gggttgcttt tcgccgcaca
     1741 gcagcgagga cagcaggatg cggttgtagc tcggctgcgg ctcctcgccc accaccgtga
     1801 tctcgtcggc gcagccacgc gcggccagct cctcgagcag ccgcgccgcg gccatgccgc
     1861 tgccgacgat cagcagtcgc tcgcgagcca tcagaagttg tactccgccc acagccagaa
     1921 cttctcggtg tccacgttgg ccgtggtgcc gatcagcacc ggcacgtcgc cgtcggcctc
     1981 gtagttgccg tacttcaggc ccacggtgta gtgcgcgccg aactgcctgg ttccctgcag
     2041 gttccactcg ctgccgaagt cgcgcggggc accgccgcgc aacgggccgt cgtcctcggc
     2101 gtattcgtgg tacaccgcca tcagcttgat gccgtacggc gtgaggtccg ccgcgagcgt
     2161 gaggtaggtg tccacgagac cgccggcagg cgtgttgagg aacatgtcga cccagccgtt
     2221 gaaggcatgc ttggtcgcgt acggcgtctg gaagccctgc atggcgttgc cggcacggtc
     2281 cgagccgagc gtctcgcgcg ccaggcgcgc cgacaccacg ccgatgccgg tgacatagcc
     2341 cgcgccggcg tcgatcaggc tgtagtcggt gtcgaagtcc agcccggttg cattctccag
     2401 ttcctggatc gcatattccg cggtgtagag caggcgcacg ggcagcgtta ccggcagcgt
     2461 gtagttgccg tcgaatcgca ggccggccgt cgcgttctcg cggtagcgca gcgtcgggtt
     2521 gagcgcgttc accctgggcg gaaggttgcg cagcgcggca tccggctcct cgttctcgag
     2581 tgcgtagcag tacgcggaga gcttgccgaa cttcagcccg gtccatgccg cgttgagcag
     2641 gtggtcggag ctgtcgatat cgaagttgaa cgtgggttcc acgtcggcga tgccgttgac
     2701 gtggtcgacg taggcgtaga acaggctcca gtccggcacg ccggtgtagc tcgcgctgaa
     2761 ggcgtcgaag gtctgttcgt cctggcgcca gcccacgttg cccacgaagc gctggttgtc
     2821 gagcacgagg cgctggcgcc cgagccccat gtccagccgc gagatgccgc ggtagcgcag
     2881 gtaggcgcgg ttcacctcgg tgttctcggg atccaccacc acggcatagc ccgccgtttc
     2941 cggcgcgtac tcgtcctggc cgaacagcgc gcgcgtgtcc tcgaactcga ggatcgcggt
     3001 gagacccttc tgcggcgcgg tctcgatgcc gaggcggctg cgcagcgtca gcgccgaggc
     3061 gtcgtccacg gtggccgaat cgaggtccac cgattcctgg cgcacgcgga agttgcccga
     3121 taccttgctc ttcatcagcg catcggtcag cggatcggct tcggcctgcg cagccgacgc
     3181 cgcgatcacg gccagggcca gcaggtggtg cgcgccgcgg cgcatcgtgc gattcatcat
     3241 gtcggtactc cggtgaatgc tgcgttgaaa caggggctca ggcgacatgc ttgccgtgca
     3301 gcggcgtcac cacggcgcgt tcgccacgcg ccggttgcgg catctgcgcg ccggcgggcg
     3361 ccttcgcggc gagcggcgcg tgcgccggct ccggctggct cgcgccgggc gaggcgcgcg
     3421 cggttccatc caccttgcgc tgcttctcgt agaggaagcg cagcacttcc tggcggcaat
     3481 ggttgtagcg gggatcctcg gcgagctcca ggcggttgcg cggacgttcg aggtccacgt
     3541 cgaggatctc gccgatggtg gcggcaggac cgttggtcat catcacgatg cgatccgaca
     3601 gcagcaccgc ctcgtcgacg tcgtgcgtga tcatgatcac ggtgttggcg agctcgcgct
     3661 ggatctccat cagcgagtcc tgcaggtgcg cgcgcgtcag cgcatcgagc gcaccgaagg
     3721 gctcgtccat cagcagcacg cgcggctgca tcgccagcgc gcgcgcgatg cccacacgct
     3781 gcttcatgcc gccggagatc tcgcccgggc gcttgtgcag cgcgtgctcc atgtgcacga
     3841 gcttcaggtt gtgctcgatc cactcgcgca tctcggcgcg cgatttcttc gcgcggaaca
     3901 cctgccgcac ggccagctcg acgttctggt aggcgctgag ccacggcagc agcgagtggt
     3961 tctggaacac cacggcgcgt tcgggcccgg gctcggtcac ttcccggcca tccagcacca
     4021 cgccaccggt ggtggcccgc aacaggccgg ccacgatgtt cagcacggtc gacttgccgc
     4081 agcccgagtg cccgatcagc gacacgtact cgccctcgcg gatcttgagg ttgacgttgt
     4141 cgagcgcctt gaagggcccc ttgtcggtct tgaactcgat ccctacctgg ctcagctcca
     4201 gcagtgtctt cacggtacga tctcctggtg aattgatctc gcggcggcgc ggcatcagcg
     4261 cgtcacggcg ttcttgtccc acgacaccac gcgctgcagc gcgagcatca cgcgatcgag
     4321 caggaagccg atcaggccga tcgtgatcac cgcgaccatg atccggccga gcgactgcga
     4381 gctgccgttc tggaactcgt cccagatgaa cttgccgagg cccgggttct gcgacagcat
     4441 ctcggcggcg atcagcacca tccacgccac gccgagcgac agccgcaggc ccgtgaagat
     4501 catcggcacg gccgccggca gcacgatgcg gcgcacatgc tgcagccagc ccaggcgcag
     4561 caccttgctc acgttgttca ggtcgtcact caccgaggcc acgcccaccg cggtgttgat
     4621 cacggtcggc cacacgcaac agagcgcgac ggtgatcaac gaggtgatga aggacttggg
     4681 caccgcgggg tcgggattgt cgtggaccga ggccaccacc agcgtcacca gcggcaacca
     4741 ggcgagcggc gacaccggct tgaatacctg gatcaagggg ttcaccgcgc catggagcgg
     4801 cccgctcagc ccgatcgcga tgcccaccgg gatcgccagc agcgccgaga gcaggaatcc
     4861 gctcatcacg gtcttcaggc tggtcacgat ctgggtgtaa aacgtggggg acgcggtgta
     4921 gggtcgcacc ggtgccgccg cggccccggg attggctgcc gccatcttcg ccgcgcgctc
     4981 ctgctggcgc gcgtggaagg ccgcttccct ttcgcgatcc atcaggtatt cgcggtgcag
     5041 acccttcgct tcgctccaca ccagcgccgg acccggcaac tgccccagcg tggtctgcac
     5101 ccgcgccgcg cccgcctgcc acagcaacag gaagatcgcg atgccgagca gcggcatcgc
     5161 cagcgcacgc gtgaggctcg cgagctggcg cccggcgccg cgcgcatccc acgcccgggc
     5221 gcgggcggcg aaccaggcgg gcgttctcgt cgtctccatt ctcttcactc ctgcggatgg
     5281 atgtcggatt cgtcggcgac gcgactcagg gttgcgcgct gcccttcagg ccgatcgcga
     5341 agctgtcgat gtaggcattg ggctgcgtgc cgtcgtagac gatgccatcg agcggcccgg
     5401 cctgaggcgc cttgatgaac gtcgccttgt cgaggtccgg gaagtcggag gcctgcatca
     5461 cgccatcggc aatgagttcc ttcgcggccg ccacgtagat gtcggggcgg aaggcgcgct
     5521 tcgcggtctc gaggtaccag ctgtcgggct ggtgctccgt gatgtggccc cagcggcgca
     5581 tctgcgtcag ccaccagatc gcgtcggagt agtaaggaat gccgtactgg ccgcggaaga
     5641 acttgttgaa gtccggcacc gggcgcttgt cacccttctc gaactcgaag gtgccggtca
     5701 tgctgttggc gatgaccttt tcgtcggcgc ccacgtacgc cgggcgcgcg atgatcttca
     5761 ccgcttcggg gcgattcgcg ttgccgttct cgtccagcca gtacgaggcg cggatcaacg
     5821 ctttcaccac gcgcaccgtg gtgttcgggt tcttctgcac gaactcgccg gtcatgccga
     5881 acaccttgtc ggcgccgtcg gtccagatgt tctcgctggt gatcaccggc accccgagcc
     5941 ccttggtcac cgcctgctgg ttccacggct cgccgacgca gtagcccgcc gtcgtgcccg
     6001 cctccatcgt cggcaccatc tgcggcggcg gcaccaccga gagcagggcc tcggctccca
     6061 gcgaaccgct gttgtcgccc ttctgcggtg cgtacacgcc cgggttgatg ccgcccgcgg
     6121 ccagccagta gcgcaggtgg taattgtgcg tacccaccgg gaaggtcatg gccaggttga
     6181 aagtcctgcc ctgcgccttg tacgactcga gcaccggctt cagcgcggcc gcgctgaccg
     6241 gatgcttcgg cttgccgtcc tccaccggaa cggtcttttc catctcggcc cacaccgcgt
     6301 tcgacacggt gatggcggca ccgttgtagg agagcgtgaa cggggtgacg atttccgact
     6361 tcgtgatcac gccggtcgcc gcacccagcg gcatcggcgc cagcatgtgc gcaccgtcca
     6421 gctcgccgct gacgacacgg tcgaacagca ccttccagtt ggcctgtgcc tcgagcgtga
     6481 cgtagagacc ctcgtcctcg aagtaaccgc gctcgtaggc gatggccagc ggcaccatgt
     6541 cggtgagctt gatgaagccg aacttcagct cccacttctc cggttcgccg atcgcggccg
     6601 atgccggcgc gccggccagc agcccggccg cgcagaggcc gaggatcccc gccacccttg
     6661 cacgccatcc gcgagtcctg ccctcgtgtc gtcctgtcat gtgcgctctc cgcatgaaaa
     6721 tcgatccaaa aaaaatgccc acgtaccgcg tcggcggttc gtgggcatca ttacccggtg
     6781 gttgagaggt tcgggctcgg cacctgcgaa gaagcaagtg cccggcctga aatcagcaat
     6841 gcagtttgcg tgccagtata atttctctca tagtttcaat cagttgcacc gaaaccgtcc
     6901 tggtcctgac cctgaaatgc gcgcgccggg caatccaaag tggtgcgcgc tgcaccgcgc
     6961 gcgggcatcg gatcagcccg cggacaggcg aaactgcccg ccgccgatca tcaggtcggg
     7021 tccgagcacc acgccgggct cgaactccca gcacccggcg tgcagcccct cgggcctggc
     7081 gtcgcgcgag ggcgcgggca gcccgagatg gcgcgccgcc tcgcggtaga ggtcgggccg
     7141 ccaggactgc tgcaccagcg cgcggcattg ctcctcggcg atgtccttgc ccagcagcgc
     7201 gccgctgctc ctgaccatcc attgcgcctg cgagcgccac gggaagccgg cgcaatagcg
     7261 gccgaagaca tgaaaatccg gcgcatccat cggcgcgagg tgcttggcgc gacagaacct
     7321 gcctgtcagc gcgggcagca gcaccttcgc cggcaacccg aggtactgcg cctggctgag
     7381 gatgtccgcg gcgagttcgc gctgcgcggg ctcggccagc gcctggcagg cggccatgat
     7441 cgcgatgcgc aggcgcaggt gcgtggccgg atggtggttg tgccaggcct cggtgacgcc
     7501 gagcaccttt tccggcgcgt tgttccagat ctcgtggccg gtcgcggcca ccgcgccgat
     7561 gccctgctgc accgcgagcg tgttccacgg ctcgccgacg cagaagccgt cgatgatgcc
     7621 gcgcgcgaga ctgtcgcaca tctgctcggg cggcagcacg atgatgcgca cctcgcgatc
     7681 gggatccacg ccgccggcgc gcagccacat gcgcagctgg aaggtgtgca tcgagaacag
     7741 gtgcacggtg gcgagcgtga gcggcccggc gcccgggctg ccggctatcc gctcgtgcag
     7801 tgccgcggcc atgcccgggg cgctggcgtc gcgctcaccg ccgagcgctt cccacagcgc
     7861 ccgcgacagc gtgatcgcgt tgccgttcag gccgagcgaa aggccggtca gcaggttggc
     7921 gcgcatgccg ccggcgccga gcccggtcat catcggcagc ggcgccagca ggtgcgcggc
     7981 gtcgagcttg cccaccacca gcttgtcgcg cagattcgcc cacgacacct cgcgcgcgag
     8041 cgcgacctcg agcccgtaac gcgcgaacag cccggtttca cgcgccatca ccagcggcgc
     8101 gctgtcgctg agacgcatga agcccagcgt cagcgcggac ttttccggcg gcggaagttg
     8161 cttgtcgcga ctcatcggct cctgcccttg cccttgcccc cgggcgacag cgtggccagc
     8221 acggtcctgg ccacgtccgg cagtcgctgg ttggcgtcca tcgcgagccg ggtgagcatc
     8281 cggtgtgccc cgtcctcgct gagtttctgg taggccatca gcaggccctt ggcctgctcg
     8341 atcaacttgc ggtcctcgag ctggatgcgc gtgtcgtgca gttcctcgcg cagcgcctgg
     8401 aaggcgcgga actgcgcgat cgcgacgtcg atcacgggct tgacctgctc gggattgatg
     8461 ccgcccacca ggtaggtgct gatgcccgcg ccgaccgcct gctcgatgta gtccgggtcg
     8521 tcctgctgcg tgaacatcac caccggacgc gggttgtggt gattcacgac ggcgaggctc
     8581 tccagcacgt cgcggtccgg cgactcgatg tcgatcagta tcacgtcggg cgcgtgctgc
     8641 tggatctgga acagcagccc cttcgccgag gcgatgacgg ccaccacctc gaagcccgcg
     8701 gcccacagcc tctcctcgac cagttgcgcg cgctccggac tgtcgtccac cagcatcacg
     8761 cgggcgggtg gcttgctgct gtcggtgttc aaccaggtgt tccggcgggg gaatctcagc
     8821 gcaataccat caacattcgt gccatcaggc ttcgtgatgc acattgcgcc attcccgtgc
     8881 gcgctcggcg tgccgactcc ggctggtcgc gcaccaaagc gaggcgtatg cacgccagct
     8941 gcaccatggc gcggcacagg ggcacgcatt tcccgctggc acgcgtcgta cgtttccgcg
     9001 ttaagctcgg cccttgactc tggaccatgg tcaagggtgc agagtgcctg cacacaggag
     9061 atcggcaatg tccacgtaca gcatcggtga actcgccagg cgcgccggcg tcgccatcga
     9121 cacggtgcgc tactacgagc gcaaccagct gctcgacccg gccgggcgcc tcgcctccgg
     9181 ctaccgccgc tatggcgaca gcgaactgag gcgactgcgc ttcatccggc gcgccaaggc
     9241 gctgggcttc tcgctcgcgg acgtgcgcgc gctgctgtcg ctgagcgatg agcgcaacgt
     9301 cgccaaggtc aagcgcgccg ccgaagcgcg gctggccgag gtcgagcagc gtctcgccga
     9361 actggaacgc atccgcaacg cgctgcaggc gctggtagcc gcctgccccg gccatggccg
     9421 cgcggaggcc tgcccgatcc tcaacgctct gaaccaggag gacgccccgt gaacacgctc
     9481 cattcccccg ccccgtcgcc ggcggatgcc tcggcgcact gcagtcacgg ccatgcggcc
     9541 gcgactttca tcgacccggt ctgcggcatg accgtgaagc cggagtcgcc gcaccacgcc
     9601 gtgcacgccg ggcgcgaata ccgtttctgc tccgcgggct gccgcggcaa gttcgtcgcc
     9661 gacccggccc gctggctcga accgcgcgat ccggagcctg ccaccgccct acccggcgcc
     9721 acgtacacct gcccgatgca tcccgagatc atccgcgacc acccgagcag ctgcccgc
//
LOCUS       DAORUQ010000121         9627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7553_length_9627_cov_5.956749, whole genome shotgun sequence.
ACCESSION   DAORUQ010000121 DAORUQ010000000
VERSION     DAORUQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_9627_cov_5.956749"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLW13_10955"
     CDS             complement(<1..423)
                     /locus_tag="PLW13_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70938.1"
                     /translation="MKTGLWMQRTALMFLLGCAGALAQAACPEHTEGFTCREYESPPR
                     HASTPPGRYVMYFYIETCPHCQDARPKVDAWRKTLPQDLKYIEHVAKWRDREGAAFAY
                     RIESVPSFVVDGKYLIAIDSWEPAHTDAAIALVSELAGL"
     gene            complement(558..1895)
                     /locus_tag="PLW13_10960"
     CDS             complement(558..1895)
                     /locus_tag="PLW13_10960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY70939.1"
                     /translation="MRVFPTVAIALTLAASCVSAAELSAVVHRPSVEVHAQPVFDSPT
                     LATLQRDARVSISSQQGLWYELQMPAGKSGYVRVNDVRLAYAGAEAGEANVQVLMSGK
                     AGVGRITETAGVRGIDESDLKSSAFDTAQLDAMTAQRVDGPVAAGYAQEQGWEATQVD
                     FAGEAEARRADPAEQPAAARAETVKAVGGLLGSLGGGLGSGLGSMLGGASRLVPKSEG
                     ELAGEELALGPEITGRILGARPLWNDTAAQRRVNLVGRWVASQTSRPELPWTFGVIDT
                     PEVNAFAAPGGFILVTRGLYQLLSSDSELAAALGHEISHCVQRDHYNVIRKQELASSG
                     KELAMSKVELGNSSPAAAYARNYVETHGATIMMSALDREAEYRADEGAEHYLARAGMN
                     PLALYSLLQKLTALGSDSAMLAQLYKTHPPLDERIDRIDQRGFGALQQYTMRE"
     gene            complement(1895..3739)
                     /locus_tag="PLW13_10965"
     CDS             complement(1895..3739)
                     /locus_tag="PLW13_10965"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQY70940.1"
                     /translation="MPGRLADRIFSWIDASLAALGHGFIQQWTKVERSYRRPLSWLAF
                     RLKFAFYPLLALGAIAWLAWDWSDTRSLDSAEDAIFDQVVQWRPFEPKPSGRVVVVEI
                     DECSIAYFRARGEGGWPWSRQRHADLLDQLDRAGVRAVGYDVLFADPSQDDPLGDQTL
                     EAMALGGAGRFVFGSTRLHPDYDESSRLRASQAPGAFALVPAPRVDPRVALLLPYGEA
                     MTRYSAIANVSRNKDGVLRDIPLRESAGDWALPSLALRLALSAMPPSAHPPAAAVRPN
                     WRQDTRLPHVSAADLLSGGKAVCRDASGSLPALNGRVALVGYTASGLNDAKPTPVDPV
                     MPGVEVLAEATEALLADSAIRTPPGWLKYVLATLLTLLTTFAFWRGEPAPDIDSIFVA
                     VNAALLGAAFVGLTFFGFFFDIFASVGFVSLVFGSCRAYAGVQRGRAVGNGDFLREFA
                     ADQDRWLAVARLRFVPDAQLDDTSSARRQREYQRRLRRFLYAGSEAVMLEGVVERKSW
                     LHDALSDTMILVWHGANAAAARTAALADLARLERGLAEYDERLPDDGSVLLAFACARI
                     DDEPGSSGDEERLRLRELIGRVLATPTEWPLTRRSAISAATDSSPGGA"
     gene            complement(3832..4170)
                     /locus_tag="PLW13_10970"
     CDS             complement(3832..4170)
                     /locus_tag="PLW13_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70941.1"
                     /translation="MAQNKLYVGNFPYSVDEAQLRSLFSTYGDITDIALIMDRETGRP
                     KGFGFITFATQAAAEAALELDGRDLGGRPLKVNMAMERDSRSGGGGRPGGGGRPGGGG
                     RPGGGGGGRW"
     gene            complement(4189..4380)
                     /locus_tag="PLW13_10975"
     CDS             complement(4189..4380)
                     /locus_tag="PLW13_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70942.1"
                     /translation="MAKPNYSFEKRQREIAKKKKKEEKLQEKNQRKGSKGPAPDDVTA
                     EPSLTAPAPADADGDAGNN"
     gene            4585..5298
                     /locus_tag="PLW13_10980"
     CDS             4585..5298
                     /locus_tag="PLW13_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HQY70943.1"
                     /translation="MQLERILQRNGFGTRRACRVLIAQGRVAIGGVVRADPAAEIATE
                     GLVFSVDGVDWPHAEFATVVLNKPAGYECSRQPQHHRSVLELLPLPLRERGVQPVGRL
                     DADTTGLLLLTDDGPLNHALASGKRMVAKVYRVTTKHPLDERQIGQLRAGVVLRDNPE
                     PVAAVACEQLGAHHLRLTVSEGKYHQVKRMLAAVGNRVEALHREAVGGFVLPPDLAPG
                     EWRWLSAAELLQLGQCGTR"
     gene            complement(5333..6655)
                     /locus_tag="PLW13_10985"
     CDS             complement(5333..6655)
                     /locus_tag="PLW13_10985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY70944.1"
                     /translation="MQFKDLGLIEPLLQALDELGHATPTPIQLKAIPAVLAGRDLIAA
                     AQTGTGKTGSFALPLLQKLGELPPPEPNSISALVLTPTRELAQQVADDLREYGRRLPV
                     RVLVAYGGVSLNPQMMALRRGADILVATPGRLLDLHGKNALRLGQLRVLVLDEADRML
                     DMGFSRELEQILALLPRRRQTLLFSATFSDPIRTLASGLLHHPLEVDASPRNTAVASV
                     KQRVIPVDKQRKLELFCHLLRRQRWPQVLVFAKTRKRVDELVTSLQYQEVSTAAIHGD
                     IPQHARLAALRRFNAGQVRVLVATDVAARGLDIEALPLVVNLDLPIAPEGYVHRIGRT
                     GRAGQEGEAISLVCADEAMQLAAIESLIGKMLARREVEGFEPKHRVPATGPNKRPPPV
                     VDAKPAKPRAGKKKKKSAATGKPKSRAATPRTTGKAAKPAPARPPRQT"
     gene            complement(6681..7886)
                     /locus_tag="PLW13_10990"
     CDS             complement(6681..7886)
                     /locus_tag="PLW13_10990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY70945.1"
                     /translation="MSAIGSAGFSLADEARRMRRRLIAHIEAGGTTDLVTGTLDVPAS
                     VYTDPARFAAEQHDIFGELPLLAGLSGDIARPGELMLFDAAGPEIIIVRGHDGTVRAF
                     LNICRHRGARLVTHCEPRQRISCPFHNWNYDLDGRLVGIPVGAAFDGLDRAAHGLLRV
                     PVAEWHGMIFVIARPGEETIDVAGFLGPAAPLFAALDLAGATPVHADTLPVRANWKLA
                     LDSFCEPYHVPAAHPRTLAPALVPWVAIYDRFGIHQRYASPGAEVKGYAGMPDTELPE
                     PYYQGVHSLFPNTTFTVGRLVGIGNREPFIAFYRIFPGDSVGEAVAHGSIYLPRGGDP
                     ATIPDLERAHASIMQVVSGEDFVIAADSWKRLASAPPGYRLTFGRNEMLLQQNHRLIA
                     DLIGMPIVS"
     gene            complement(7975..9345)
                     /locus_tag="PLW13_10995"
     CDS             complement(7975..9345)
                     /locus_tag="PLW13_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HQY70946.1"
                     /translation="MKTRTWALLALLGAIAAAHAAAAPVTPQQFAWRFAIEAPRQAPL
                     QRLALDEALYGSVARADLGDLRVFNAEGEVLEHAIVAPREPAAAAREPVALRLFPLRV
                     GDTPAGSGSLRIRLDASGALLDLSGPDTGAQPVSAYLIDAGTEHAAIAALRLDWRDDS
                     PDFVSTVTLESSTDLRAWRAAGTAAVASLTHAGQRLARHEIALHDSPDRYLRLQWPAA
                     AKGVELSGASALLRERAAARARHWTRIEGAAAADGPHAREFDARGWLPVDRLRIAFAE
                     PNSMATLRVLSRPAVTEPWRERADALFYRLQAEGTELASPELTIERRRDRYWRIEARE
                     GNGSAPALELGWAPDELVFIARGGAPFLLAAGSARIAAAAQPVERLLADLDATRREAL
                     TGSAALGGRSRLAGPDALRPLPPPVPWQRYLLWALLIGAVLVLLAMALRLYRQMNPPP
                     AGPPAA"
     gene            complement(9342..>9627)
                     /locus_tag="PLW13_11000"
     CDS             complement(9342..>9627)
                     /locus_tag="PLW13_11000"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQY70947.1"
                     /translation="HALHRSPVFQGSISVLWALTALAVMFVATRSARRWLWLCGGALL
                     AALVLKLFFVDLAGSGTVARIVSFLGAGGLMVLIGYLSPAPPRQARETQA"
BASE COUNT         1359 a         3366 c         3455 g         1447 t
ORIGIN      
        1 gaggcccgcg agctcgctga ccagcgcgat cgcggcatcg gtgtgcgcgg gctcccagct
       61 gtcgatggcg atcaggtatt tgccgtcgac gacgaagctc ggcacgctct cgatgcgata
      121 cgcgaacgcc gctccctcgc gatcgcgcca cttcgccacg tgctcgatgt acttcaggtc
      181 ctgcggcagc gtcttgcgcc aggcatcgac cttgggtcgc gcgtcctggc agtgcgggca
      241 ggtctcgatg tagaagtaca tgacgtagcg tcccggcggc gtcgaggcgt ggcgcggcgg
      301 actctcgtac tccctgcagg tgaagccttc ggtgtgctcg gggcaggctg cctgcgccag
      361 cgcgcccgcg cagccgagca gaaacatcag cgcggtgcgc tgcatccaca gtcctgtttt
      421 cacttcgtgt ctccgccatt gccaggtcat gcgcctgccg tttcgccgcg agttcgcggt
      481 gaccggcatc cggcgcgaac gcggcattgt ccacgaggcg ccggcttcgc gccagcgcct
      541 ccccgccggg ggcggtctca ctcgcgcatc gtgtactgct ggagtgcgcc gaagccgcgc
      601 tggtcgatgc ggtcgatgcg ctcatcgagg ggcggatgcg tcttgtagag ctgggccagc
      661 atggccgagt cgctgcctag cgcggtgagc ttctgcagca gcgagtacag cgccagcggg
      721 ttcatgccgg cgcgcgcaag gtaatgctcg gcgccctcgt cggcgcgata ctcggcctcg
      781 cgatccagcg cgctcatcat gatcgtggcg ccgtgcgtct cgacgtagtt gcgcgcatag
      841 gcggccgccg ggctgctgtt gcccagttcg accttgctca tcgcgagctc cttgcccgag
      901 ctcgccagct cctgcttgcg gatcacgttg tagtggtcgc gctgcacgca gtgactgatc
      961 tcgtggccca gcgccgccgc cagctcgctg tcggaggaca gcagctggta cagcccgcgc
     1021 gtgaccagga tgaagccgcc cggcgcggcg aaggcgttga cctcgggcgt gtcgatcacg
     1081 ccgaaggtcc acggcagttc tgggcgactg gtctgcgagg caacccagcg tccgaccagg
     1141 ttgacgcgcc gctgtgcggc cgtatcgttc cacaacggcc gcgcgccgag gatgcggccg
     1201 gtgatttccg ggccgagcgc gagttcctcg ccagccagtt cgccctcgga tttggggacc
     1261 agcctggagg cgccgcccag catcgagccg aggccgctgc cgagccctcc gcccagcgag
     1321 ccgagcaggc cgcccacggc cttcacggtc tcggccctgg ccgcggcagg ctgctccgcc
     1381 ggatcggcgc gccgcgcctc ggcctcgccg gcaaagtcca cctgcgttgc ttcccagccc
     1441 tgctcctgcg catagccggc ggccacgggg ccatcgacgc gctgggccgt catcgcgtcg
     1501 agttgcgcgg tatcgaacgc ggaggatttc aggtcgctct cgtcgatccc gcgtaccccc
     1561 gcggtctcgg tgatccgtcc cacgccggcc ttgccgctca tcagcacctg cacgttggct
     1621 tcgccggcct cggcgccggc gtaggcgagg cgcacgtcgt tgacgcgcac gtagccggat
     1681 ttcccggcgg gcatctgcag ctcgtaccac aggccctgtt gcgaggaaat cgataccctg
     1741 gcatcgcgct gcagggtcgc gagcgtgggc gagtcgaata cgggttgcgc atgcacttcc
     1801 acggacgggc ggtgaacgac ggcggacaat tcggccgcgc tcacgcacga cgccgccagc
     1861 gtcaacgcaa tcgcgacggt ggggaaaacg cgcatcacgc tcctcctggg gaagaatcgg
     1921 ttgcggcgga aatggcgctc cgccgtgtca gtggccattc ggtcggggtg gcgagcacgc
     1981 gcccgatcag ctcgcgcagg cgcaggcgct cctcgtcgcc cgaggacccc ggctcgtcgt
     2041 cgatacgtgc gcaggcaaat gccagcagca cgctgccatc gtcgggcagg cgctcgtcgt
     2101 actcggccag tccccgctcg agccgcgcga ggtccgccag cgctgcagtg cgcgccgcgg
     2161 ctgcattcgc gccgtgccat accagaatca tcgtatcgct cagcgcatcg tgcaaccaac
     2221 tcttgcgttc gaccacgccc tccagcatca ccgcctcgct gccggcgtac aggaaccgtc
     2281 gcagccggcg ctggtactca cgctggcgcc gcgccgagga ggtatcgtcg agttgcgcat
     2341 cgggcacgaa gcgcaggcgc gccacggcga gccagcggtc ctggtcggca gcgaattccc
     2401 gcaggaaatc gccgttgccg accgcgcgtc cgcgctgaac gcccgcgtag gcgcgacagg
     2461 atccgaacac caggctgacg aaaccgacac tggcgaagat gtcgaagaag aagccgaaga
     2521 acgtgaggcc gacgaacgcg gcgcccagca gcgcggcgtt gaccgcgacg aagatcgagt
     2581 cgatgtcggg agccggttcg ccgcgccaga aggcaaaggt ggtgagcagc gtcagcaggg
     2641 tggcgagcac gtatttcagc cagccgggtg gtgtgcggat cgcgctgtcg gcgagcaggg
     2701 cctcggtggc ctcggccagg acctcgacgc ccggcatgac cggatcgacg ggcgtgggct
     2761 tggcgtcgtt gaggccggag gcggtgtagc cgaccagcgc cacgcgcccg ttcaacgcag
     2821 gcaggctgcc cgatgcatcg cggcagacgg ccttgccgcc gctcagcagg tcggccgcgc
     2881 tgacatgcgg cagccgcgtg tcctgccgcc agttcggccg caccgcggcg gcgggcggat
     2941 gcgccgatgg cggcatggcg ctcagcgcca ggcgcagcgc cagcgagggc agggcccagt
     3001 cgccggcgga ctcgcgcagg gggatgtcgc gcagcacccc gtccttgttg cgcgatacgt
     3061 tggcgatcgc actgtagcgg gtcatcgcct cgccgtaggg cagcagcagt gcgaccctcg
     3121 gatccacgcg cggagccggc acgagcgcaa atgcccccgg ggcctgcgag gcgcgcaggc
     3181 gcgaactctc gtcatagtcc gggtgcagcc gtgtcgagcc gaacacgaag cgcccggcgc
     3241 cgcccagtgc catggcttcc agggtctggt cgccgagggg gtcgtcctgc gagggatcgg
     3301 cgaacagcac atcgtagccg accgcgcgca ccccggcgcg atccagctgg tcgagcaggt
     3361 cggcgtggcg ttgccggctc cacggccagc cgccttcgcc gcgcgcgcgg aaatacgcga
     3421 tggagcactc gtcgatctcg accacgacca cgcggccaga cggcttcggt tcgaacgggc
     3481 gccattgcac gacctggtcg aagatcgcgt cctcggccga gtcgagcgag cgggtgtcgc
     3541 tccagtccca ggccagccag gcaatcgcgc cgagggcgag cagcgggtag aacgcgaatt
     3601 tcaggcggaa agcgagccat gacaagggcc ggcggtagct gcgctcgacc ttggtccatt
     3661 gctggatgaa gccgtggccg agcgccgcga ggctcgcatc gatccacgag aatatgcggt
     3721 ccgccagcct gccgggcatc gacacttccc tgcgctcagg tcatccacaa aaaagcccgg
     3781 caattgccgg gctctcttgt ttgcgtcgcg gggacgcggg gagggctgga cttaccagcg
     3841 gccaccgccg ccgccgccgg gacggccacc gccacccgga cggccaccgc cacccggacg
     3901 gccaccgccg ccgctgcggc tgtcacgctc catcgccatg ttgaccttga gcggacgacc
     3961 gcccaggtca cggccgtcca gctccagtgc tgcttcagcg gccgcctgcg tggcgaaggt
     4021 gatgaagccg aagcccttcg ggcgaccggt ttcacgatcc atgatcagcg cgatgtcagt
     4081 gatgtcgccg tacgtcgaga acagtgagcg caactgcgcc tcatccaccg agtacgggaa
     4141 gttgccgacg tacagtttgt tctgtgccat gtaaatagag ctccgatttc agttattacc
     4201 agcgtcaccg tcggcatcgg caggtgccgg tgctgtcaac gacggttcgg ccgtcacgtc
     4261 atccggcgca gggcctttgg agcctttgcg ctggttcttc tcctgcagtt tctcttcttt
     4321 tttcttcttc ttggcgatct cgcgctgccg cttttcgaaa gaataattgg gtttggccac
     4381 gattcacctg caggaactaa agggtttcga gcggggggga atgcgattgc ccggaccaag
     4441 ggggaagcgc aaacggcccg cgggcagtac atacgttgcg ccgatactac cccgaatcgc
     4501 gcgggattgc gcgttttttt ctgaggtagg gcggaaagtg ggccgatcct gccggcgggc
     4561 cggccgggtg ctagcattcc cgccatgcag ctcgaacgta tcctccagcg caacggcttc
     4621 ggcacccgcc gcgcctgccg cgtgctcatc gcgcagggac gggtggcgat cgggggcgtc
     4681 gtccgtgccg accccgcggc cgagattgcc accgaggggc tggtgttctc ggtcgacggg
     4741 gtggactggc cccatgccga attcgccacc gtggtgctga acaagcctgc gggctacgag
     4801 tgctcgcgcc agccgcagca ccaccgcagc gtgctggagt tgctgccgct gcccctgcgc
     4861 gaacgcggcg tacagccggt cgggcgcctc gacgcggaca ccacggggct gttgctgctg
     4921 accgacgacg gcccgctcaa ccatgcgctg gcctcgggca agcgcatggt ggcgaaggtc
     4981 taccgggtca ccacgaaaca tccgctggac gagcggcaga tcgggcaact gcgcgccggc
     5041 gtggtgctgc gcgacaaccc cgagcccgtg gctgccgttg cctgcgaaca actcggggcg
     5101 catcatctgc gcctgaccgt gagcgagggc aagtaccacc aggtcaagcg catgctggcg
     5161 gcggtgggca accgggtcga ggcgctgcac cgcgaggccg tgggcggctt cgtgctgccg
     5221 ccggacctgg cgccgggcga gtggcgctgg ctgtcggccg ccgagctgct gcaactcggc
     5281 cagtgcggca cgcgctgaat cccggcccac ccgcgcgtcc ggtgggcggc attcaggtct
     5341 ggcgcggcgg acgcgccggc gccggctttg cggccttgcc cgtcgtgcgc ggcgtcgccg
     5401 ccctcgattt cggtttgccg gtggcggccg attttttctt cttcttgccg gccctgggct
     5461 tggccggctt cgcatccacg acgggcggcg ggcgcttgtt cggccctgtc gccggcacgc
     5521 ggtgtttcgg ctcgaagccc tcgacttcgc gccgcgccag catcttgccg atcaggctct
     5581 cgatcgcggc cagctgcatc gcctcgtcgg cgcacaccag cgagatcgcc tcgccctcct
     5641 gcccggcgcg gccggtgcgc ccgatgcggt gcacgtagcc ctcgggggcg atcggcaggt
     5701 cgaggttcac caccagcggc agcgcctcga tgtcgaggcc tcgcgccgcc acgtcggtgg
     5761 cgaccagcac ccgcacctgc ccggcgttga agcgcctgag cgcggcgagc cgcgcgtgct
     5821 gcgggatgtc gccgtggatc gcggcggtgc tcacctcctg gtactgcaga ctggtgacca
     5881 gttcgtccac gcgcttgcgc gtcttcgcga acaccagcac ctgcggccag cgctggcggc
     5941 gcagcaggtg gcagaacagt tccagcttgc gttgcttgtc caccgggatc acgcgttgct
     6001 tcacgctcgc caccgcggtg ttgcgcgggc tggcgtcgac ctcgagcggg tggtgcagca
     6061 gcccgctcgc cagcgtgcgg atgggatccg agaaagtggc cgagaacagc agcgtctggc
     6121 gccgccgcgg cagcagcgcg aggatctgct cgagctcgcg gctgaagccc atgtccagca
     6181 tgcggtcggc ctcgtcgagc accagcaccc gcagctgccc gaggcgcagc gcgttcttgc
     6241 cgtgcaggtc cagcaggcgt ccgggtgtcg cgaccaggat gtcggcgccg cggcgcaggg
     6301 ccatcatctg cgggttcagg ctgacgccgc cgtaggcgac gagtacgcgc accggcaggc
     6361 ggcggccgta ctcgcgcaga tcgtcggcca cctgctgcgc gagctcgcgc gtcggggtga
     6421 gcaccagggc gctgatgctg ttgggctcgg gcggtggcag ctcgcccagc ttctgcagca
     6481 gcggcagcgc gaagctgccg gtcttgccgg tgccggtctg ggcggcggcg atcaggtcgc
     6541 gcccggcgag caccgcgggg atggctttca actggatcgg cgtgggcgtg gcgtgcccga
     6601 gctcgtccag ggcttgcagc agcggctcga tgaggccgag atccttgaat tgcatgggcg
     6661 gtgtgtccgg tgtggggcgc tcaggaaacg atcggcatcc cgatcaggtc ggcgatcagg
     6721 cggtggttct gctgcagcag catctcgttg cgcccgaagg tgagccggta gccgggcggc
     6781 gcgctcgcga gccgcttcca gctgtcggcg gcgatcacga agtcctcgcc gctgaccacc
     6841 tgcatgatgc tcgcgtgcgc cctttcgaga tccgggatgg tggccgggtc gccgccgcgc
     6901 ggcaggtaga tcgagccgtg cgccaccgcc tcgccgaccg aatcgccggg gaagatgcgg
     6961 tagaaggcga tgaagggctc gcggttgccg ataccgacca gtcgccccac ggtgaacgtg
     7021 gtgttcggga acagcgagtg cacgccctgg taatagggct cgggcagttc ggtgtccggc
     7081 atgccggcgt agcccttcac ctcggcgccc ggcgaggcat agcgctggtg gatgccgaag
     7141 cggtcgtaga tcgcgaccca cggcaccagt gcgggcgcca gggtgcgcgg gtgcgccgcg
     7201 ggcacgtggt agggctcgca gaaggaatcg agcgcgagct tccagttggc gcgcacgggc
     7261 agcgtgtcgg cgtgcaccgg tgtggcgccg gcgagatcca gcgccgcgaa cagcggcgcg
     7321 gcgggcccga ggaacccggc gacgtcgatc gtctcctcgc ccgggcgcgc gatcacgaag
     7381 atcatgccgt gccattcggc caccggcacg cgcagcaggc cgtgcgccgc gcggtcgagt
     7441 ccgtcgaagg ccgccccgac cgggatgccg accaggcggc cgtcgaggtc gtagttccag
     7501 ttgtggaacg ggcagctgat gcgctggcgc ggctcgcagt gcgtcaccag ccgtgcgccg
     7561 cggtggcggc atatgttgag gaaggcgcgt accgtgccgt cgtggccgcg cacgatgatg
     7621 atctcggggc ccgcggcgtc gaacagcatc agctcgccgg ggcgcgcgat gtcgccggac
     7681 agccccgcca gcagcggcag ctcgccgaag atatcgtgct gctcggcggc gaagcgcgcc
     7741 gggtcggtgt agacgctggc cggcacgtcc agtgtcccgg tcaccaggtc ggtggtgccg
     7801 ccggcctcga tgtgcgcgat caggcgccgg cgcatgcggc gcgcctcgtc ggccagcgag
     7861 aaacccgcgc tcccgatcgc gctcatgcct gctcccgtgc gtgtcgtgct gtcatggccg
     7921 cgcattgtgc ggcatggagc gggcgcgcgc gaggcgcgcc gccatcggcc gtgttcaggc
     7981 ggcaggcggt cccgccggcg gcgggttcat ctgccggtac agccgcagcg ccatcgccag
     8041 cagcaccagc acggcgccga tcagcagcgc ccacagcagg tagcgctgcc acggcaccgg
     8101 cggcggcagc ggtcgcaggg cgtcgggacc ggcgaggcgg ctgcgtccgc ccagcgcggc
     8161 gctgccggtc agcgcctcgc gccgcgtcgc gtcgagatcc gcgagcaggc gctcgaccgg
     8221 ttgcgcggcc gctgcgatgc gcgcgctgcc cgcggcgagc aggaacgggg cgcccccgcg
     8281 cgcgatgaac accagttcgt ccggcgccca gccgagctcc agcgccggcg cgctgccgtt
     8341 gccctcgcgc gcctcgatgc gccagtagcg gtcacgtcgg cgctcgatgg tcagctcggg
     8401 gctcgccagt tccgtgccct cggcctgcag ccggtagaac agcgcatcgg cgcgctcgcg
     8461 ccacggctcg gtgacggcgg ggcgcgacag cacgcgcaag gtcgccatgc tgttgggctc
     8521 ggcgaaggcg atgcgcagcc ggtcgacggg cagccagccg cgcgcgtcga attcccgggc
     8581 gtgcggccca tccgccgccg cggcgccctc gatgcgcgtc cagtgccgcg cgcgtgctgc
     8641 ggcgcgctcg cgcagcaagg cgctcgcgcc cgacagttcc acgcccttgg cggccgcggg
     8701 ccattgcagg cgcaggtagc ggtccgggct gtcgtgcagt gcgatctcgt ggcgggcgag
     8761 acgctgcccc gcgtgcgtga ggctcgccac cgccgccgtg cccgcggcgc gccacgcacg
     8821 caggtccgtg ctgctctcca gcgtcacggt gctgacgaaa tcgggggagt cgtcgcgcca
     8881 gtccagcctg agcgcggcga tcgcggcgtg ctccgtgccg gcgtcgatca ggtaggcgct
     8941 gaccggctgc gcgccggtgt ccgggcccga caggtcgagc agcgcgccgc tggcgtcgag
     9001 cctgatccgc agcgagccgc tccccgccgg cgtgtcgccg acgcgcaacg ggaacaggcg
     9061 cagcgccacg ggttcgcgcg ccgcggccgc gggttcgcgc ggcgccacga tcgcgtgttc
     9121 cagcacttcg ccctcggcat tgaacacgcg caggtcgccc aggtcggcgc gcgccacgct
     9181 gccatagagc gcctcgtcga gcgcgagacg ctgaagcggc gcctgccgcg gcgcctcgat
     9241 cgcgaagcgc cacgcgaact gctgtggcgt caccggcgcc gcggccgcat gcgccgcagc
     9301 gatcgcgccg agcagcgcca gcaaggccca cgtccgggtc ttcatgcctg tgtctcccgc
     9361 gcctggcgcg gcggcgccgg tgacaagtag ccgatcagta ccatcaggcc tcccgcgccg
     9421 aggaacgaga cgatgcgcgc cacggtgccg ctgcccgcca gatcgacgaa gaacagcttc
     9481 agcaccagcg ccgccagcag cgcgccgccg cagagccaca gccagcgccg ggcgctgcgc
     9541 gtggcgacga acatcaccgc gagcgccgtg agcgcccaca gcaccgagat gctgccctgg
     9601 aataccggcg agcggtgcag cgcgtgc
//
LOCUS       DAORUQ010000122         9608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7581_length_9608_cov_6.222548, whole genome shotgun sequence.
ACCESSION   DAORUQ010000122 DAORUQ010000000
VERSION     DAORUQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_9608_cov_6.222548"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1230)
                     /gene="apbC"
                     /locus_tag="PLW13_11005"
     CDS             complement(136..1230)
                     /gene="apbC"
                     /locus_tag="PLW13_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HQY70948.1"
                     /translation="MSAIDLQAARRVLASCRVEGIEAPLGEWTELTALERRGDIAVAT
                     VHAGIPAAGLREPIAAVVRHALAPLVAGAALRMDFEVRIAPTPRRGDAPGLDSVRNLI
                     AVASGKGGVGKSTTAVNLALALQREGARVGLLDADIYGPSQQIMLGVPEHQRPEGREG
                     NFIVPIEAHGLQTMSMGYLVTEKTAMVWRGPMASGALQQMIAQTLWRDLDYLVVDMPP
                     GTGDIQLTLAQKAPVSGAVIVTTPQDIALLDCKKGIEMFSKVGIPVLGVVENMAVHVC
                     SHCGHAEHLFGAGGGERIAREYGVPLLGALPLDIRIREQTDGGKPTVAADPDGPLGAI
                     YRDVARRMGLELLRRNAAQGQVPSITVTND"
     gene            1395..3431
                     /gene="metG"
                     /locus_tag="PLW13_11010"
     CDS             1395..3431
                     /gene="metG"
                     /locus_tag="PLW13_11010"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQY70949.1"
                     /translation="MPAQRRILVTSALPYANGPLHLGHMIEQIQTDIWVRFQRLRGHA
                     CTYVCASDAHGTPVMLRAEKEGLTPEQLIERIQASHRADSAGFLVSCDNFYTTHSPEN
                     RHFAELIYTRLAARGHIARRSIEQAFDPEKGLFLPDRYIKGGCPRCGAADQYGDSCES
                     CGATYTPLDLKNPVSVLSGATPVRRESEHFFFRLGDFEAMLREWTAGEGVQPEVANKL
                     QEWFSQGLNDWDISRDAPYFGFEIPGEPGKYFYVWVDAPIGYMASFRDLCNRTGEDFD
                     SYWAEGGSTELYHFIGKDIAYFHTLFWPAMLSGAGFRRPDAVFCHGFLTVNGQKMSKS
                     RGTFIMARTYLEHLDPEYFRYYIAAKLGAGLEDIDLNLEDFVLRVNSDLVGKVVNIAS
                     RCAGFISKRFEGRLAAGDEAAALLADFLAERDTVAAYYEGREYGKAIRLIMALADRAN
                     QYIDHEKPWALAKDPAQSARVQAVCSAGIELFRVLMIYLAPVLPAMAAKAAGFLRSEL
                     DWAALAGPLGAHTIDTFSPLMNRVDPERVAAMVEASREDVAQGSAPAVAAAPVAAQHP
                     PIAGTVGIEDFAKIDLRVARILAAEPVEGADKLLALTLDLGDDIRRNVFAGIKSAYAP
                     AELIGRLTVVVANLAPRKMRFGTSEGMVLAAGPGGKDIFLLAPDSGAVPGMQVK"
     gene            3453..4028
                     /gene="rsxA"
                     /locus_tag="PLW13_11015"
     CDS             3453..4028
                     /gene="rsxA"
                     /locus_tag="PLW13_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HQY70950.1"
                     /translation="MSDLALFLVGAVLVNNFVLVQFLGLCPFMGVSSKLESALGMAMA
                     TTFVLTLTSTCTHLVFRYVLAPLGLEYLQTISFILVIAAVVQFTEMFVRKTSPLLYKV
                     LGVFLPLITVNCAVLGVALLNVNKHHDFLHSVLYGFGAAAGFSLVLVLFAAMRERIAA
                     ADVPQPFRGAAIGMITASLMSLAFMGFAGLV"
     gene            4025..4636
                     /gene="rsxB"
                     /locus_tag="PLW13_11020"
     CDS             4025..4636
                     /gene="rsxB"
                     /locus_tag="PLW13_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HQY70951.1"
                     /translation="MIELLTQHPVAAALVALAALALGFGALLGFASVRYAVTGNPIVE
                     QILEKLPQTQCGQCGHPGCRPYAEAIAAGEPINKCPPGGQATINALADLLHVEAPALD
                     AEHGEEKPRTVAFIREDECIGCTKCIQACPVDAILGAAKQMHTVIASECTGCDLCVEP
                     CPVDCIEMRPVPSTAQGWSWELPRRAAPQTSLIASDAPAERAA"
     gene            4633..6552
                     /gene="rsxC"
                     /locus_tag="PLW13_11025"
     CDS             4633..6552
                     /gene="rsxC"
                     /locus_tag="PLW13_11025"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQY70952.1"
                     /translation="MRRLSDIPGGIHPPQNKTQSLRHPIARAPLAPELVLPLSQHSGA
                     PAEPVVAPGDQVLKGQMIARASGALSAALHAPSSGEIVAIEPRPVPHPSGMDAMAIVL
                     RPDGAERWIERRPMPDYRERTREEVLAKIRDAGVAGLGGAGFPTALKLAPGPARRIRT
                     LIINGAECEPYITADDMLMRERAAEVVAGARIIAWLLEAGETLIAIEDNKPEAIAAMR
                     AAAGTDIEVVPFPTKYPSGGEKQLIQIMTGQEVPSGKLPADIGICCQNVGTAAEIHRA
                     VERGEPLIARITTVTGAAVDDPRNFEVLLGTPISLLLAAAGFHPERAARLVMGGPMMG
                     FALPHAELPVVKTTNCVLAPSAAELPLPPPAQACIRCAMCAQVCPASLLPQQLFWYAQ
                     AEDHVNLEKQHLFDCIECGACSWACPSSIPLVQYYRASKGAIRQAREQQQKAENARRR
                     FEQRQARIEQQEQEKEARRAARQQEARKPATPAAEAAAAPAPAPAAAVAAAAPAAAPA
                     VAPAPDPLAAARASQVEAAVERVKAKKLAAAGEHRDVVADAIERARRQALERAATIPA
                     APAPAAEPPSRETLERRVATMEQKLARATEKLLAAEAAGETETVALLSRARGDLEKRL
                     ADARQELEMLAAVQE"
     gene            6560..7600
                     /gene="rsxD"
                     /locus_tag="PLW13_11030"
     CDS             6560..7600
                     /gene="rsxD"
                     /locus_tag="PLW13_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HQY70953.1"
                     /translation="MALPRFSSPHAHGPARTSWVMQQVLLATVPGVLALTVCFGWGTL
                     ANILWAVPVALACEGAMLRLRGRDVRFSLRDCSALVTALLLAIALPPGSPWWLVTTGT
                     AFAIVIAKQLYGGLGSNPFNPAMAGYVVLLISFPLEMTRWMAPAAAGIAAPLGPLEAL
                     QASLGLLDAARLDALTMATPLDVLRQNQSLLVSELWAKNPQFGRIAGAGWEWVNAGFL
                     AGGLYLLARRIFTWHAPLAMLGALALMAAIFHDGGSAASGGSPLFHLFSGATMFGAFF
                     IVTDPVTCATSNRGRLIFGALVGVLVYLIRHWGNYPDAVAFAVLLMNFAAPLLDHYTQ
                     PRSYGHDRGRSA"
     gene            7597..8244
                     /gene="rsxG"
                     /locus_tag="PLW13_11035"
     CDS             7597..8244
                     /gene="rsxG"
                     /locus_tag="PLW13_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HQY70954.1"
                     /translation="MKLADAVGRNSLVLGLVALLITLALAGTQLLTREQIAAQRRAAE
                     ARAYNEILPPARYDNVLLDDFRLVDDRELLGLQQARKALVARLGGEAQAVIVPATAPD
                     GYGGAIELIVGINADGTVAGVRVVAHKETPGLGDKIDARKSRWIDSFAGRSLADPAPA
                     QWTVHKDGGVFDQFTGATITPRAVTAAVKRTLQYFEANREALLAPAATGSEARHE"
     gene            8237..8953
                     /locus_tag="PLW13_11040"
     CDS             8237..8953
                     /locus_tag="PLW13_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HQY70955.1"
                     /translation="MSEPRIGEITLNGLWKNNPALVQLLGLCPLLAVSGSVVNALGMG
                     LATALVLMGTNTLVSMVRHVVPDTIRLPAFVLIIAGAVTLVELLMQAYAYGLYEVLGI
                     FLPLITTNCVVLGRAEAFASKNPVPLAALEGLMMGLGFSAVLVVLGALRELLGNGTLF
                     ADMQLLFGPMAADWEVRPFGEQRHFLLAVLPPGAFLFTGLLIALKNGIDAQIKARAEA
                     ARAPQVPAGSKRVRVTGHIS"
     gene            8950..9597
                     /gene="nth"
                     /locus_tag="PLW13_11045"
     CDS             8950..9597
                     /gene="nth"
                     /locus_tag="PLW13_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQY70956.1"
                     /translation="MNGAKRREIFRRLQAANPAPTTELEFASPFELLVAVILSAQATD
                     ISVNKATRKLYPVANTPAAILALGEEGLKPYIRHIGLFNSKAKNVIATCALLIERHAS
                     QVPAERKALEALPGVGRKTANVILNTAFGEPTMAVDTHIFRLANRIRLAPGKTVRAVE
                     ENLLKTTPPEYLRDAHHWLILHGRYVCTARNPNCRECVISDLCEYPGRKKILQQP"
BASE COUNT         1442 a         3470 c         3287 g         1409 t
ORIGIN      
        1 gcgaatccac ctgtccgatt tgatcgacat cgctgtgctg cttcccgatt gagtcgtggc
       61 gcccggttcg atgcccgcgc cggggggcgc catcttagcg gctggcgggg gccgcgcaaa
      121 gccgcgcgcg caagctcagt cgttggtgac ggtgatgctg ggcacctgcc cctgcgccgc
      181 attgcgccgc agcagttcca gccccatgcg acgcgccacg tcgcggtaga tcgcgccgag
      241 cgggccgtcg ggatcggcgg ccacggtggg cttgccgccg tcggtctgct cgcggatgcg
      301 gatgtcgagc ggcagcgcgc cgagcagcgg cacgccgtat tcgcgcgcga tgcgctcgcc
      361 gccgccggca ccgaagaggt gctcggcgtg cccgcaatgc gagcacacgt gcaccgccat
      421 gttctcgacc acgccgagca ccgggatgcc gaccttggag aacatctcga tacccttctt
      481 gcagtcgagc agcgcgatgt cctgcggcgt ggtgacgatc accgcgccgc tgaccggcgc
      541 cttctgcgcg agggtcagct gaatgtcgcc ggtgcccggc ggcatatcga cgaccaggta
      601 gtcgaggtcg cgccaaagcg tctgcgcgat catctgctgc agcgcgccgc tggccatcgg
      661 gccgcgccag accatcgcgg tcttctccgt gaccaggtag cccatcgaca tggtctgcag
      721 gccgtgcgcc tcgatcggca cgatgaaatt gccctcgcgc ccctcgggac gctggtgctc
      781 gggcacaccg agcatgatct gctggctggg gccgtagatg tcggcgtcca gcagcccgac
      841 ccgcgcgccc tcgcgctgca gggcgagcgc caggttcacc gcggtggtcg acttgccgac
      901 gccgcccttg cccgaggcga ccgcgatcag gttgcgcacg ctgtcgagcc cgggcgcatc
      961 gccgcgccgt ggcgtgggcg cgatgcgcac ctcgaagtcc atgcgcagcg cggcgcccgc
     1021 caccagcggt gcgagggcat ggcgcaccac cgccgcgatc ggctcgcgca gcccggcggc
     1081 gggaatgccc gcgtgcacgg tggcgaccgc gatgtcaccg cgccgctcca gcgccgtgag
     1141 ttcggtccat tcgccgagcg gcgcctcgat gccttccacg cggcaactgg cgagtacgcg
     1201 gcgggccgcc tgcaggtcga tggcgctcat gtctgactcc cggaaagcgg aaaatgccgc
     1261 atcttagcgg cctcgccacg ggcgcggaag gcgcgccgcg cgggccggta ctatccacga
     1321 tgcaaagccc gggggatatc gttatatttc ccgtttttac cgcgggaatc ccgcgcaacg
     1381 gaccccatcg cgccatgccc gcccaacgcc gcatcctcgt taccagcgcc ctgccctatg
     1441 ccaacgggcc gctgcacctc ggccacatga tcgagcagat ccagaccgac atctgggtgc
     1501 gcttccagcg cctgcgcggg cacgcctgca cctacgtgtg cgcgagcgac gcgcacggca
     1561 ccccggtgat gctgcgcgcc gagaaggaag ggctgacacc agagcagctg atcgagcgca
     1621 tccaggccag ccaccgcgcc gattccgccg gcttcctggt gagctgtgac aatttctaca
     1681 ccacgcactc ccccgagaac cgccacttcg ccgagctgat ctacacccgc ctcgccgcgc
     1741 gcggccacat cgcgcggcgc tcgatcgagc aggccttcga ccccgagaag ggcctgttcc
     1801 tgccggatcg ctacatcaag ggcggctgcc cgcgctgcgg cgcagccgac cagtacggcg
     1861 attcctgcga atcctgcggc gccacctaca caccgctcga cctgaagaac ccggtctccg
     1921 tgctctcggg cgccacgccg gtgcggcgcg agtcagagca cttcttcttc aggctcggcg
     1981 atttcgaggc gatgctgcgc gaatggaccg ccggcgaggg cgtgcagccc gaagtggcga
     2041 acaagctgca ggagtggttc tcccaaggcc tcaacgactg ggacatctcg cgcgacgcgc
     2101 cctatttcgg cttcgagatc ccgggcgagc cgggcaagta tttctacgtc tgggtcgatg
     2161 ccccgatcgg ctacatggcg agcttccgcg atctgtgcaa ccgcaccggc gaggacttcg
     2221 acagctactg ggccgagggc ggcagcacgg agctctacca cttcatcggc aaggacatcg
     2281 cgtatttcca cacgctgttc tggcccgcga tgctgagcgg tgcgggcttt cgccgccccg
     2341 acgcggtgtt ctgccacggc ttcctcacgg tcaacgggca gaagatgtcg aagtcgcgcg
     2401 gcaccttcat catggcgcgc acctacctgg agcacctcga tcccgagtat ttccgctact
     2461 acatcgcggc caagctcggc gcggggctgg aggacatcga cctcaacctc gaggacttcg
     2521 tgctgcgcgt gaattcggac ctggtcggca aggtcgtgaa catcgcgagc cgctgcgccg
     2581 gcttcatctc caagcgcttc gagggccggc tcgccgccgg cgacgaggcc gccgcgctgc
     2641 tggcggactt cctcgccgaa cgcgacaccg tcgccgctta ctacgaaggc cgcgaatacg
     2701 gcaaggcgat ccgcctgatc atggcgctcg ccgaccgcgc caaccagtac atcgaccacg
     2761 aaaagccctg ggcgctggcg aaggaccccg cccagtccgc gcgcgtgcag gcggtgtgct
     2821 cggcgggcat cgagctgttt cgcgtgctga tgatctatct ggcgccggtg ctgccggcaa
     2881 tggccgcgaa ggctgccggc ttcctgcgca gcgaactgga ctgggccgcg ctcgcgggcc
     2941 cgctcggcgc gcacaccatc gacaccttca gcccgctgat gaaccgtgtc gaccccgagc
     3001 gcgtggccgc gatggtcgag gccagcaggg aagacgtggc gcaggggagc gcaccggcgg
     3061 tggcagccgc accggtggcg gcgcagcatc cgccgatcgc cgggaccgtc ggcatcgagg
     3121 acttcgcgaa gatcgacctg cgcgtggcgc gcatcctggc ggccgaaccg gtggaaggcg
     3181 ccgacaagct gctggcgctg acgctggacc tcggtgacga catccgccgc aatgtgttcg
     3241 cgggcatcaa gtccgcgtat gcgccggccg aactcatcgg acggctcacg gtggtggtcg
     3301 ccaacctcgc gccgcgcaag atgcgcttcg gcacctccga gggcatggtg ctggcggccg
     3361 gccccggcgg caaggacatc ttcctgctcg cgcccgacag cggcgcggtg ccgggaatgc
     3421 aggtcaagta gagcgcgggg gcctcgcaga tgatttccga tctcgcgctg ttcctggtcg
     3481 gcgccgtgct ggtcaacaac ttcgtgctgg tgcagttcct cgggctgtgc cccttcatgg
     3541 gcgtgtcctc gaaactggaa tccgcgctcg gcatggcgat ggcgaccacc ttcgtgctga
     3601 cgctcacctc gacctgcacc cacctggtgt tccgctacgt gctcgcgccg ctcgggctgg
     3661 aatacctgca gacgatctct ttcatcctgg tgatcgcggc ggtggtgcag ttcaccgaga
     3721 tgttcgtgcg caagaccagc ccgctgctgt acaaggtgct cggcgtgttc ctgccgctga
     3781 tcacggtgaa ctgcgcggtg ctgggcgtgg cgctgctgaa cgtcaacaag caccacgact
     3841 tcctgcactc ggtgctgtac ggcttcggcg ccgccgcggg cttctcgctg gtgctggtgc
     3901 tgttcgcggc catgcgcgag cgcatcgccg cggccgacgt gccgcaaccc ttccgtggcg
     3961 ccgcgatcgg catgatcacg gccagcctga tgtcgctcgc cttcatgggc ttcgccggcc
     4021 tcgtatgatc gagctgctca cccagcaccc cgtcgcggcg gcgctcgtcg cgctcgccgc
     4081 gctcgcgctc ggcttcggcg cgctgctcgg cttcgccagc gtgcgctacg cggtcaccgg
     4141 caacccgatc gtcgagcaga tcctcgagaa gctgccgcag acgcaatgcg gccagtgcgg
     4201 acaccccggc tgccggccct acgccgaggc catcgccgcc ggcgagccga tcaacaagtg
     4261 tccgcccggc gggcaggcca cgatcaacgc gctcgccgac ctgctgcatg tcgaagcgcc
     4321 cgcgctcgac gccgagcacg gcgaggagaa gccgcgcacg gtggccttca tccgcgagga
     4381 cgagtgcatc ggctgcacca agtgcatcca ggcctgcccg gtggacgcga tcctcggcgc
     4441 cgccaagcag atgcacacgg tgatcgcctc ggagtgcacc ggctgcgatc tgtgcgtcga
     4501 accctgcccg gtggactgca tcgagatgcg cccggtgccg agcacggcac agggctggag
     4561 ctgggaactg ccgcgccgcg ccgcgccgca gacatcgctc atcgcgagcg atgcgcccgc
     4621 cgagcgcgcg gcatgaggcg gctcagcgac atccccggcg gcattcatcc gccgcagaac
     4681 aagacgcagt cgctgcgcca cccgatcgcg cgcgcgccgc tggcgccgga actggtgctg
     4741 ccgctctcgc agcacagcgg cgcgccggcc gagcccgtcg tcgcgccggg cgatcaggtg
     4801 ctgaagggac agatgatcgc gcgcgccagc ggcgcgctga gcgcagcgct gcacgcgccg
     4861 agttcgggcg agatcgtggc gatcgagccg cggcccgtgc cgcacccctc cggcatggac
     4921 gcgatggcga tcgtgctgcg ccccgacggc gccgagcgct ggatcgagcg ccggccgatg
     4981 ccggactacc gcgaacgcac gcgcgaggag gtgctggcga agatccgcga tgccggcgta
     5041 gccgggctgg gtggcgcggg cttcccgacc gcgttgaagc tggccccggg gccggcgcgg
     5101 cgcatccgca cgctgatcat caacggcgcg gaatgcgagc cctacatcac ggccgacgac
     5161 atgctgatgc gcgagcgcgc ggccgaggtc gtggccggtg cgcgtatcat cgcgtggctg
     5221 ctggaggccg gggaaaccct gatcgcgatc gaggacaaca agcccgaggc gatcgcggcg
     5281 atgcgcgcgg cggcggggac ggacatcgag gtcgtgccgt tcccgaccaa gtacccctcg
     5341 ggcggcgaga agcagctgat ccagatcatg acgggccagg aagtgccctc cggcaagctg
     5401 cccgccgata tcggcatctg ctgccagaac gtcggcaccg cggcggagat ccaccgcgcg
     5461 gtcgaacgcg gcgaaccgct gatcgcgcgc atcaccacgg tgacgggcgc ggcggtggac
     5521 gatccgcgca acttcgaggt gctgctgggc acgccgatca gcctgctgct ggccgcggcg
     5581 ggcttccatc ccgagcgcgc ggcgcggctg gtgatgggcg ggccgatgat gggctttgcg
     5641 ctgccgcacg ccgaactgcc cgtggtcaag accaccaact gcgtgctcgc gccgagcgcc
     5701 gcggaactgc cgcttccgcc gccggcgcag gcctgcatcc gctgcgcgat gtgcgcccag
     5761 gtctgcccgg cctcgctgct gccccagcag ctgttctggt acgcgcaggc cgaggaccac
     5821 gtgaacctcg agaagcagca cctgttcgac tgcatcgagt gcggcgcctg ctcgtgggcc
     5881 tgcccgagca gcattccgct ggtgcagtac taccgtgcct cgaagggcgc gatccgccag
     5941 gcccgcgagc agcagcagaa agccgagaac gcgcgccggc gcttcgaaca gcgccaggcc
     6001 cgcatcgagc agcaggagca ggaaaaggag gcgcgccgcg ccgcgcgcca gcaggaggcg
     6061 cgcaagcccg ccacgcccgc cgccgaagcg gcagcagcac ccgcacccgc ccctgcagca
     6121 gcagtggcag ccgcagcgcc cgctgccgcg cctgccgtcg cgccggcacc ggacccgctc
     6181 gccgccgccc gcgccagcca ggtcgaggcg gcggtggagc gcgtgaaggc gaagaagctc
     6241 gcggccgccg gcgagcaccg cgacgtggtg gccgacgcca tcgagcgcgc gcgccgccag
     6301 gcgctggagc gcgccgccac gatccccgcc gcgccggcgc ccgccgccga gccgccgtcg
     6361 cgcgagaccc tcgagcgccg cgtcgcgacg atggaacaga aactcgcgcg cgccaccgaa
     6421 aaactgctcg ctgccgaagc cgcgggcgag accgaaaccg tcgcgttgct ctctcgcgcg
     6481 cgcggcgacc tcgaaaagcg cctcgccgat gcgcggcagg aactcgaaat gctcgccgcg
     6541 gtgcaggagt agcacgacca tggccctgcc ccgcttcagc tcgccgcacg cccacgggcc
     6601 ggcgcgcacc tcatgggtga tgcagcaggt gctgctggcg acagtgcccg gtgtgctcgc
     6661 gctgacggtg tgcttcggct ggggcacgct ggcgaacatc ctctgggcgg tgcccgtggc
     6721 gctcgcctgc gagggcgcga tgctgcgcct gcgcgggcgc gacgtgcggt tcagcctgcg
     6781 cgactgcagc gcgctggtga ccgcgctgct gctcgcgatc gcgctgccgc cgggctcgcc
     6841 gtggtggctc gtcacgacag gtaccgcgtt cgcgatcgtg atcgcgaagc agctctacgg
     6901 cggcctcggc agcaacccgt tcaacccggc aatggccggc tacgtggtgc tgctgatatc
     6961 gttcccgctg gagatgacgc gctggatggc gccggcagcg gccggcatcg ccgcgccgct
     7021 cggtccgctc gaggccctgc aggcctcgct gggactgctc gatgccgcgc gcctcgacgc
     7081 gctgaccatg gccaccccgc tcgacgtgct gcgccagaac cagtcgctgc tggtctccga
     7141 gctgtgggcg aagaacccgc agttcggccg catcgccggc gccggctggg aatgggtcaa
     7201 tgccggcttc ctcgccggcg ggctctacct gctggcacgg cgcatcttca cctggcacgc
     7261 gccgctcgcg atgctgggcg cgctggcgtt gatggccgcg atcttccacg acggcggcag
     7321 cgcggccagc ggcggctcgc cgctgttcca cctgttctcg ggcgcaacca tgttcggcgc
     7381 gtttttcatc gtgaccgacc cggtgacctg cgcgaccagc aaccgtggtc ggctgatctt
     7441 cggcgcgctg gtcggcgtgc tggtctacct gatccggcac tggggcaact atcccgacgc
     7501 ggtggccttc gccgtgctgc tgatgaactt cgccgcgccg ctgctcgacc actacacgca
     7561 gccgcgcagt tacggccacg accgcgggag atccgcatga agctggccga cgccgtcggg
     7621 cgcaacagcc tcgtgctggg actggtcgcg ctgctcatca ccctggcgct ggcgggcacg
     7681 cagctgctga cgcgcgagca gatcgccgcg cagcggcgcg ccgccgaagc gcgcgcctac
     7741 aacgagatcc tgccgccggc gcgctacgac aacgtgctgc tcgacgattt ccgcctggtc
     7801 gatgaccgcg agctgctcgg actgcagcag gcgcgcaagg cactggtggc gcgtctgggc
     7861 ggcgaagcgc aggcggtgat cgttccggcc acggcgcccg acggctacgg cggggcgatc
     7921 gagctgatcg tcggcatcaa cgccgacggc acggtggccg gcgtgcgcgt ggtggcgcac
     7981 aaggagacgc cggggctcgg cgacaagatc gatgcccgca agtcgcgctg gatcgactcc
     8041 ttcgcgggcc gctccctcgc cgatcccgcg ccggcgcaat ggacggtgca caaggacggc
     8101 ggcgttttcg accagttcac gggcgcgacg atcacgccgc gcgcggtgac cgcggcggtc
     8161 aagcgcacgc tgcagtattt cgaggccaac cgcgaggcgc tgctcgcccc cgcggcaaca
     8221 gggagcgaag cccgccatga gtgagccccg catcggcgaa attacgctga acggcctgtg
     8281 gaagaacaac ccggccctgg tgcaattgct cgggctgtgc ccgctgctcg cggtcagcgg
     8341 ctcggtggtc aacgcgctcg gcatggggct cgcgacagcc ctcgtgctga tgggcaccaa
     8401 cacgctggtg tcgatggtgc gccacgtggt gcccgacacg atccgcctgc ccgccttcgt
     8461 gctgatcatc gccggcgcgg tgacgctggt cgaattgctg atgcaggcat acgcctacgg
     8521 gctctacgag gtgctgggca tcttcctgcc gctgatcacc acgaactgcg tggtgctcgg
     8581 gcgcgccgag gccttcgcca gcaagaaccc ggtgccgctc gcggcgctcg aggggctgat
     8641 gatggggctg ggcttcagcg ccgtgctcgt ggtgctcggc gcactgcgcg agctgctcgg
     8701 caacggcacg ctgttcgccg acatgcaact gctgttcggc ccgatggccg ccgactggga
     8761 ggtccgcccc ttcggcgagc agcgccactt cctgctcgcg gtgctgccac ccggcgcctt
     8821 cctgttcacc gggctgctga tcgcgttgaa gaacggcatc gacgcgcaga tcaaggcgcg
     8881 cgccgaggcc gcgcgcgcgc cgcaggttcc cgccggcagc aagcgcgtgc gcgtcaccgg
     8941 gcacatcagt tgaacggcgc gaagcgccgc gagatcttcc gccggctgca ggccgccaac
     9001 ccggcgccga ccaccgagct cgagttcgct tcgcccttcg agctgctggt cgcggtgatc
     9061 ctctcggcgc aggccaccga catcagcgtg aacaaggcca cgcgcaagct ctacccggtg
     9121 gccaacaccc ccgcggcaat actcgcgctc ggcgaggaag ggctgaagcc ctacatccgc
     9181 cacatcggac tgttcaacag caaggcgaag aacgtgatcg ccacctgcgc gctgctgatc
     9241 gaacgccacg catcgcaggt gcccgccgag cgcaaggcac tcgaggcact gcccggcgtg
     9301 ggccgcaaga ccgccaacgt gatcctcaac accgccttcg gcgagccgac catggccgtc
     9361 gacacgcaca tcttccgcct cgccaaccgt atccgtctcg cccccggcaa gacggtgcgc
     9421 gcggtcgagg aaaacctgct caagaccacg ccgcccgagt acctgcgcga tgcgcaccac
     9481 tggctgatcc tgcacgggcg ctacgtctgc accgcgcgca acccgaattg ccgcgagtgc
     9541 gtgatcagtg acctctgcga ataccccggc cgcaagaaga tcctgcagca gccgtaggtc
     9601 ggcattca
//
LOCUS       DAORUQ010000123         9470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7766_length_9470_cov_6.126819, whole genome shotgun sequence.
ACCESSION   DAORUQ010000123 DAORUQ010000000
VERSION     DAORUQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7766_length_9470_cov_6.126819"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLW13_11050"
     CDS             complement(<1..436)
                     /locus_tag="PLW13_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70957.1"
                     /translation="MPAIGLTGPAVLQGKPAKENRMSTGKVLDPTAVRADDIASPGPD
                     AGSLAGKIVGLRLDEIWRAWDWIAKIWAEEFSRAGAEVKFWRSNQGRTGAEGERMARE
                     LDEFLNAIDIAVVGLGNCGSCTGWTIRDALAAADKGLPTTAVC"
     gene            522..1259
                     /locus_tag="PLW13_11055"
     CDS             522..1259
                     /locus_tag="PLW13_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQY70958.1"
                     /translation="MLVKPIANLFSLMDFFVRARRPLSVRDIVAEFSWPRSSVFNMVS
                     TMVEHGYLHQPVPRGGYYPTTRWMELARDLSESQPLPESVHKLLVELANRSGETLFLA
                     AAEGTSAVFLDVVESSADIRFIANVGQRLPIHVTAAGRAILAQYSPAERAATLKRIKY
                     QPYEKDTFMTPESVERDVRESARNGWYVNPGIYAPGVAGIAVPFPFRGRRNAIALGGP
                     ASRIEKHVDDLGKLLRESIDRFLAVNE"
     gene            1256..2170
                     /locus_tag="PLW13_11060"
     CDS             1256..2170
                     /locus_tag="PLW13_11060"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY70959.1"
                     /translation="MKAEAARGSRKRQKNEVLEDMQMIDIPVEPISPGLGARVLIRAE
                     HLLDEGVAQQCSNLLDEHLVLVFPQIAVSDDVQIAFSNRLGKMQGSRLRAKADTAADK
                     MGIYPVTLDPGRAKFLDYIHSNEHWHMDGTTYDIPPRATNLKCEVPPASGGDTEFANL
                     FAAYDDLPASRKPELENLRVVHSAATANLRSFSKPSVLDLQRWLSDGPPHEQPLVWQQ
                     ASGRRSLVIGSTADHIVGMDLDKGRALLDELLAWCTQPRYCYRHTWQKGDMVIWNNPA
                     LLHRAHHYTADSGRLMHRTTIMGTEAFA"
     gene            2238..2996
                     /locus_tag="PLW13_11065"
     CDS             2238..2996
                     /locus_tag="PLW13_11065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HQY70960.1"
                     /translation="MTGLSNKVAVVTGGAAGIGAAVCRFLARDGADIAIWDWNADAAR
                     DLVHEIAAMGRKVVACKVDVSSKNDIESAVKAVHDQLGPVGILVNNAAISPEKDFVDI
                     TPQDWDRVFDINTKGVFMCTQAVIDDMITARWGRIINISSSSAQSGARRMVHYAASKG
                     AVIAFTKSLAQEVGALGITVNNIPPSTVYTAGLQSVEARLPGGIEGYVNSTIPVKRVG
                     QPEDIANAVAFLASEASGYITGLTLSVNGGRYMQ"
     gene            3125..3415
                     /locus_tag="PLW13_11070"
     CDS             3125..3415
                     /locus_tag="PLW13_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HQY70961.1"
                     /translation="MLKHNSYFDGNVQSVGFSRHGLLATVGAIAPGEYRFNTGDAERM
                     TVVSGQLHARLAGGEWQSYAAGTYFEVPANSSFDVQAVGGPAAYLCEFLGKP"
     gene            3458..>3865
                     /locus_tag="PLW13_11075"
     CDS             3458..>3865
                     /locus_tag="PLW13_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQY70962.1"
                     /translation="MPSTQRELHARSLADPEGFWGEAARAIDWNVAPRTVLDRSRTPF
                     DRWFPDGELNACHNALDRHVDAGRGGEAALIYDSPVTGTVRSYTWAELRDEVARLAGA
                     LQEMGVTRGDRVLVYMPMIPEAVMAMLACARIGA"
     assembly_gap    3866..3965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3966..>5102
                     /locus_tag="PLW13_11080"
     CDS             <3966..>5102
                     /locus_tag="PLW13_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY70963.1"
                     /translation="VPATHPLYILYTSGTTGQPKGVVHDTGGYLVALAWSMRNIYDVL
                     PGDIFWAASDVGWVVGHSYITYGPLVNGSTTVLYEGKPVGTPDAGAFWRVIAQHRVKA
                     LFTAPTALRAVRKDDPHAKLLADYDISCLRSLFLAGERCDPETLNWAHEILQLPVIDH
                     WWQTETGWSICANCTGLGLLPVKAGSPTLPVPGYRLEVLAADGTPRAPGELGALTIAL
                     PLPPGTFTTLWNNDERFLKSYFTTYPGHYETGDAGYVDEEGYVFVMARTDDVINVAGH
                     RLSTGAIEEALATHPDVAECAVVGVHDELKGQIPLGLLVLKAGVTREHRAIVDEAVKR
                     VRERIGPVAAFKTCAIVQRLPKTRSGKILRATMRAIADGESYRPP"
     assembly_gap    5103..5202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5203..5831)
                     /locus_tag="PLW13_11085"
     CDS             complement(<5203..5831)
                     /locus_tag="PLW13_11085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY70964.1"
                     /translation="MQSITLEYTADGLSMKSDLYLPDAAGKRPGVLVFPEAFGIGDHA
                     RARARRLAEAGYVALACDLHGNAHRYDRIEEVMGLIGPLRGEVARIRARAAGGLEALR
                     ARPEVDPARIAAIGFCFGGTMSLELARGGADIRAVTGFHSGLATVAPDDAKHIRARVL
                     VCIGADDPGISAEERAAFEQEMRAGGVSWQMHLYGGVVHSFTNEKAAAMG"
     gene            complement(5942..6856)
                     /locus_tag="PLW13_11090"
     CDS             complement(5942..6856)
                     /locus_tag="PLW13_11090"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY70965.1"
                     /translation="MLADIRQQLRLPAIQAPMFLVSGPAMVIAACRAGIIGSFPTQNV
                     RDDAELERWFAEIDASTGVRGGCAPYAVNVIVHPSYPRREQDIAAVLKWKPPIVITAL
                     GSPREVVDAIHSYGGIVLADVISLRFARKALDAGVDALVLVAAGAGGHTGPIAGFAFV
                     EEVRRFYAGPLVLGGGIGSGRAIRAAEILGADFAYLGTRFLATAESMVSEEQKRMVVA
                     SGSEDIVCSDSFTGVKANWLRQSIVDYGLDPDHLPEGQIDFSRQDLKRWRDIWSAGQG
                     VGAVDRICPLAEVVAQLEREYHAACQMK"
     gene            complement(6846..8537)
                     /gene="paaN"
                     /locus_tag="PLW13_11095"
     CDS             complement(6846..8537)
                     /gene="paaN"
                     /locus_tag="PLW13_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HQY70966.1"
                     /translation="MSSWFEKHVATLDKALDACARRYSWTAYPESPSSRVHGTELPAR
                     GRREFEALLGERFGIGLLPGHDGWVGGEVSPFSREALGIRYPRLDVDTAFAAARRAWP
                     AWRDAAMNERIGICLEMLARAGDPLALFVNAHATMHTAGQSYIMAFAGCGANALDRGL
                     EALAYAWKAMRDVPQSAQWERGFGGPENARLEKHYRILPRGPAVVITCATFPQWNGYP
                     AVMANLATGNPVILKPHPNAILPVALFLRSARELLVEAGFDPNLLTMVADDRDHQVTQ
                     QLLRHPDTALIDFTGSARFGSWIEDNCRRAQVYTETAGCNSVLLESTDDLAATAQAIA
                     HSLCQASAQMCTSVQNIYIPAAGIRSGQETVSFDAVCAALRDAVDAHLRDPAKAAVLC
                     GALVNDGVAQTVEAMREQGARRGRIVRDSAPYAHPDFPAARTATPLMLAVDLAAADLY
                     GEERFGPVSFLIRAPSREAALADATARVRERGAITSHVYSTDAGFLQRAVDAYQVAGA
                     SVACNLVGMPINFAAAYSDFHVTGLTPAGNACLTDLAFVTNRFRIVQAKWRSDAR"
     gene            8653..>9470
                     /locus_tag="PLW13_11100"
     CDS             8653..>9470
                     /locus_tag="PLW13_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY70967.1"
                     /translation="MNAAHILPSSPIAKALAVVGDRWSALVMREMFLGTTRFGELAAA
                     TGASRATLSKRLRALLANGLLYQNPYQMRPQRVEYRLTEKGFGLYDFALAIWTWEARW
                     SRGADRRLPPRLLHRGCGHKTLPTACCARCGKPIDIHELRYEAGMARSAESAVNDGAL
                     RRSTRALPEAGDGKGLLLHAIDLIADHWTPLVLAAAFMGIRRYDDIRRELGIATNILS
                     HRLKLLVEAGVLQQAPGTLSGKRLEYRLSEKGRALIVPALALHQWSLKWLPAGKG"
BASE COUNT         1587 a         3173 c         3056 g         1454 t
ORIGIN      
        1 tgcagactgc ggtggtcggc aatcccttgt ccgcggcagc cagcgcatcg cgaatggtcc
       61 aaccggtaca ggatccgcag ttgcccagtc cgaccacagc gatgtcgatg gcattcagga
      121 attcgtccag ctccctggcc atgcgctcgc cttcggcacc cgtgcggccc tggttcgaac
      181 gccagaactt cacttccgcg ccggccctgc tgaactcctc tgcccagatt ttcgcaatcc
      241 agtcccacgc ccgccagatc tcgtccaggc gcaaaccgac gatcttccct gccagggagc
      301 ccgcatcggg acccggcgag gcgatatcgt ccgcgcgcac cgcggtcggg tccagtactt
      361 tgcctgtgct catcctgttc tccttcgcag gtttcccctg caacacggct gggccggtca
      421 ggccgattgc tggcatgcat cacaagctga gcgcttgaca catagtccag cattctggac
      481 tatcaaaacc attatgatgg acatactcgg atacgcggtt catgcttgtc aaacccattg
      541 ccaacctgtt cagccttatg gacttcttcg tccgagccag gagaccgctg tcggtgcgcg
      601 atatcgtcgc ggagttttcc tggccccggt ccagcgtgtt caacatggtc tcgacgatgg
      661 tggagcacgg ctacctgcat cagccggtgc cacggggcgg ctattacccg accacccgat
      721 ggatggagct ggcgcgggat ctctccgaat cccagccatt gccggaatcg gtgcacaagc
      781 tgctcgtcga gctcgcgaac cggagcggtg aaacgttgtt cctcgccgcg gccgagggaa
      841 ccagcgcggt attcctggat gttgtggagt cgtcggcgga catccgcttc attgccaacg
      901 tcggccaacg gcttcccata cacgtgacgg ccgcgggcag ggccatcctg gcccagtact
      961 cgcccgctga acgcgcggca acgctcaagc gcatcaagta tcagccctac gagaaggaca
     1021 ccttcatgac gcccgaatcg gtggagcggg atgtgcgcga gagcgcgagg aatggctggt
     1081 acgtcaatcc gggcatctac gcacccggtg tggcggggat tgccgtgcct tttccgtttc
     1141 gcggcaggcg caatgccatc gccctgggcg gacccgcttc acgcatcgag aagcatgtag
     1201 atgacctggg caagctgctg cgcgaatcga tcgatcgctt tctcgcggtc aacgaatgaa
     1261 ggccgaagcc gcgcgcggat ccaggaagag acaaaaaaat gaagtgctgg aggacatgca
     1321 gatgatcgat attcctgtag agcccatttc gcccggcctg ggtgcccgtg tgctgattcg
     1381 cgccgaacac ctgctcgacg agggcgtggc gcaacagtgc tcgaatcttc tggatgagca
     1441 cctggtgctg gtgtttccgc aaatcgctgt cagtgatgac gtgcagatag ccttcagcaa
     1501 ccggctggga aagatgcagg gctcccggct tcgcgcgaaa gcggataccg cggccgacaa
     1561 gatgggtatc tatccggtaa ccctggatcc cgggcgcgcc aagttcctcg actacatcca
     1621 ttccaacgaa cactggcata tggacggaac gacctacgac atcccgccca gggccaccaa
     1681 cctcaagtgc gaggtacccc ccgccagtgg cggtgacacg gagtttgcaa atctgtttgc
     1741 cgcctatgac gacctgccgg cgtccagaaa accggagctg gagaatctgc gtgtcgttca
     1801 cagcgcagcg acggccaacc tgagatcctt cagcaagccc tcggtcctgg acctgcagcg
     1861 ttggctcagc gatggcccgc cgcacgagca accgctggtg tggcaacagg cgagtggccg
     1921 cagatccctg gtcatcggct cgacggccga ccatatcgtc ggcatggacc tggacaaggg
     1981 gcgcgcgttg ctcgatgagc tgctcgcatg gtgcacgcag ccccgctact gctaccggca
     2041 cacgtggcag aagggcgaca tggtcatctg gaacaacccg gcactgttgc accgggctca
     2101 ccattacacc gccgattcag ggcgcctgat gcaccgcacc accatcatgg gtaccgaagc
     2161 gttcgcgtga acggggcggc cggggctggc cgccgcacga cagtgaaaat atcagtatct
     2221 gaagagaggg aactgccatg acgggtttga gcaacaaggt tgccgtggtg actggcggag
     2281 ccgcgggtat cggcgctgca gtttgtcgct ttctcgcccg cgacggcgcg gacattgcca
     2341 tctgggactg gaatgcggat gcggcgcggg acctcgtgca cgagattgcc gcgatgggcc
     2401 gcaaggtcgt ggcctgcaaa gtcgatgtgt cgagcaagaa tgacatcgag tccgccgtaa
     2461 aggccgtgca cgaccagctc ggccccgtcg gcatcctggt caacaacgcc gccatttcgc
     2521 ccgaaaagga tttcgtggat atcacgccgc aggattggga ccgggtgttc gatatcaaca
     2581 ccaaaggggt gttcatgtgc acgcaggcgg tcatcgacga catgatcacg gcccgatggg
     2641 gacggatcat caatatctcc tcctcgagcg cacagtccgg cgccaggcgc atggtgcatt
     2701 acgcggccag caaaggtgcc gtcatcgcgt tcaccaagtc gctggcacag gaagtgggcg
     2761 ccctgggaat taccgtgaac aacattcccc ccagcaccgt gtacacggcc ggactccagt
     2821 cggtcgaagc gcgcctcccg ggagggatag agggatacgt gaacagcacg atacccgtca
     2881 agcgcgtggg acagcccgag gacatcgcca acgccgtcgc cttcctcgca tccgaagcgt
     2941 ccggatacat caccgggttg accctgagcg tcaacggtgg caggtacatg cagtgatccg
     3001 gcagcccggc gctgcaggag cgatgtgcgc gcgggcaggt cgcggactta tactgccgcc
     3061 ccggccgcag gcgcgaaaac ccggcccggg agcgacgatc acccttcacg caggagcagc
     3121 aaaggtgctg aaacacaatt cctatttcga cggcaacgtg cagagcgtgg gcttttcacg
     3181 ccacggcctg ctcgcgacgg tcggcgcgat agcgccgggc gaataccgtt tcaataccgg
     3241 cgatgccgag cgcatgacgg tggtcagcgg ccagctgcac gcacgccttg cgggcggcga
     3301 atggcagagc tatgcggcgg gcacgtattt cgaggttccc gccaacagca gcttcgacgt
     3361 gcaggcagtg ggcggccccg cggcctacct gtgcgaattc ctcggcaagc cgtaggtccg
     3421 gattcatccg gacatcacgc cttcgggggg aatacgtatg ccatcgacgc aacgtgaact
     3481 gcacgcccgc tcgctcgccg accccgaggg gttctggggc gaggcggcgc gcgccatcga
     3541 ctggaacgtg gcgccgcgca ccgtgctcga ccgcagccgc acgcccttcg accgctggtt
     3601 ccccgacggc gagctgaacg cctgccacaa cgcgctggat cgccacgtcg acgccgggcg
     3661 cggcggcgag gccgcgctga tctacgacag ccccgtgacc gggacggtgc gcagctacac
     3721 ctgggcggag ctgcgcgacg aggtcgcacg gctggccggt gcgctgcagg agatgggtgt
     3781 cacccgaggc gaccgcgtac tggtctacat gccgatgatc cccgaggcgg tgatggcgat
     3841 gctcgcctgc gcgcgcatcg gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnncgtgc cggcaacgca cccgctctac atcctctaca cctcgggcac caccgggcag
     4021 cccaagggcg tggtgcacga caccggcggc tacctggtgg cgctggcgtg gagcatgcgc
     4081 aatatctacg acgtcctgcc tggcgacatc ttctgggccg cctccgacgt gggctgggtg
     4141 gtggggcatt cctacatcac ctacggtccg ctggtgaacg gctccaccac ggtgctctac
     4201 gagggcaagc ccgtgggcac gccggatgcc ggcgcgttct ggcgcgtgat cgcgcagcac
     4261 cgcgtgaaag cgctgttcac ggcacccacg gccctgcgcg cggtgcgcaa ggacgacccg
     4321 cacgcgaagc tgctcgccga ttacgacatc tcctgcctgc gctcgctgtt cctcgccggc
     4381 gagcgctgcg atcccgagac actgaactgg gcgcacgaga tactgcagct gcccgttatc
     4441 gatcactggt ggcagaccga gaccggctgg tcgatctgcg ccaattgcac cggcctcggc
     4501 ctgctgccgg tgaaggccgg ttcgccgacg ctgccggtgc cgggctaccg cctcgaggtg
     4561 ctggccgccg acggcacgcc gcgcgccccg ggcgaactgg gtgcgctcac gatcgcgctg
     4621 ccgctgccgc ccggcacctt caccacgctg tggaacaacg acgagcgctt cctgaagtcc
     4681 tatttcacga cctatcccgg acactacgag accggggacg cgggctacgt ggacgaagag
     4741 ggttacgtgt tcgtgatggc gcgcaccgac gacgtgatca acgtcgccgg gcaccggctt
     4801 tccaccgggg cgatcgagga agcgctggcc acgcaccccg acgtcgccga atgcgcggtg
     4861 gtgggggtgc acgatgaact caagggacag ataccgctcg gactgctggt gctgaaggcc
     4921 ggcgtcacgc gcgagcaccg cgcgatcgtc gacgaggccg tgaagcgcgt gcgcgagcgc
     4981 atcgggccgg tggctgcatt caagacctgc gcgatcgtgc agcggctgcc gaagacccgc
     5041 tcgggcaaga tcctgcgcgc cacgatgcgc gcgatcgccg acggcgagag ctaccgcccg
     5101 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccatcgc cgctgccttc
     5221 tcgttggtga agctgtgcac cacgccaccg tagaggtgca tctgccagct cacgcctccg
     5281 gcacgcatct cctgttcgaa ggcggcgcgc tcctcggccg agatgcccgg atcgtcggcg
     5341 ccgatgcaca ccagcacgcg cgccctgatg tgcttcgcgt catccggcgc caccgtggcc
     5401 agcccgctgt ggaaaccggt cacggcgcgg atgtcggcgc cgccgcgcgc cagctcgagc
     5461 gacatcgtgc cgccgaagca gaagccgatc gcggcgatac gcgcgggatc gacctccggg
     5521 cgcgcacgca gcgcctcgag accgccggcg gcgcgggcac ggatgcgcgc cacctcgccg
     5581 cgcaacggcc cgatcagccc catgacttcc tcgatcctgt cgtagcggtg cgcattgccg
     5641 tgcagatcgc aggcgagcgc cacgtaacca gcttcggcca ggcgccgcgc gcgtgcgcgc
     5701 gcgtgatcgc cgatgccgaa ggcttcgggg aacaccagca cgcccgggcg cttgcccgca
     5761 gcatccggca ggtacagatc gctcttcatg gacaggccgt cggcggtgta ttcgagggtg
     5821 atgctttgca tgtcgctgcg tctctccgcg tgctattcga tgtaggtcgg gcttcagccc
     5881 gacagatgtc cggctgaagc cggacccgcg atagtcggat ccggcgcgag ccgcgcccgc
     5941 atcatttcat ctggcaggcc gcatggtact cgcgctcgag ttgcgcgacg acctccgcca
     6001 gcgggcagat gcggtccacg gcacccacgc cctgccccgc cgaccagata tcgcgccagc
     6061 gcttcaggtc ctggcgcgag aagtcgatct gcccttcggg aaggtggtcc ggatcgagcc
     6121 cgtagtcgac gatcgactgc cgcagccagt tcgccttcac gccggtgaag gagtccgagc
     6181 acacgatatc ctcgctgccg gacgccacca ccatgcgctt ctgctcctcc gagaccatgc
     6241 tctccgcggt ggcgagaaag cgcgtgccca ggtacgcgaa atccgcgccg aggatctcgg
     6301 cggcacggat cgcgcgcccg ctgccgatgc cgccgcccag caccagcgga ccggcgtaga
     6361 aacgccgcac ttcctcgacg aaggcgaagc ccgcaatcgg tccggtgtgg ccgcccgcgc
     6421 cggccgcgac cagcaccagc gcgtcgacac cggcatcgag cgccttgcgc gcaaagcgca
     6481 gcgagatcac gtcggcgagc acgatgccgc cataggagtg gatcgcatcg accacctcgc
     6541 gcgggctgcc gagcgcggtg atcacgatgg gtggcttcca cttcagcacc gcggcgatgt
     6601 cctgctcgcg gcgcgggtag ctcgggtgca cgatcacgtt gacggcatag ggcgcgcaac
     6661 cgcccctgac gccggtgctg gcatcgattt cggcgaacca gcgttcgagc tccgcgtcgt
     6721 cgcgcacgtt ctgggtcggg aagctgccga tgatgccggc gcggcaggcc gcgatcacca
     6781 tcgccggccc cgagaccagg aacatgggag cctggatcgc cgggagacgc agttgttgcc
     6841 tgatgtcagc gagcatcgct tctccacttc gcttgcacga tacggaaacg gttggtcacg
     6901 aaggcgagat ccgtcaggca ggcattgccc gccggcgtca ggccggtgac gtgaaaatcg
     6961 ctgtaggcgg ccgcgaaatt tatcggcatg ccgaccaggt tgcaggcgac cgaagcgccc
     7021 gcgacctggt aggcatccac cgcgcgctgc aggaagcccg cgtcggtcga gtacacgtgc
     7081 gaggtgatcg cgccgcgctc gcgcacgcgc gcggtggcat ccgccagcgc cgcctcgcgc
     7141 gagggcgcgc ggatcaggaa ggacaccggc ccgaagcgct cctcgccgta gagatccgcc
     7201 gccgcgagat cgaccgccag catcagcggc gtggcggtgc gcgcggcggg gaagtccggg
     7261 tgcgcgtagg gcgccgagtc gcgcacgatg cggccgcgcc gggcgccctg ttcgcgcatc
     7321 gcctcgacgg tctgcgcgac gccgtcgttg accagcgctc cgcagagcac tgcggccttg
     7381 gcgggatcgc gcaggtgcgc gtcgacggca tcgcgcagcg cggcgcagac cgcatcgaac
     7441 gacacggtct cctgacccga gcggatgccc gcggccggga tgtagatgtt ctgcacgctg
     7501 gtgcacatct gcgccgaggc ctggcacagc gagtgcgcga tcgcctgtgc cgtggcggcc
     7561 aggtcatcgg tggattccag cagcaccgaa ttgcagcccg ccgtttcggt gtagacctgc
     7621 gcgcggcgac agttgtcctc gatccagctg ccaaagcgcg ccgagccggt gaaatcgatg
     7681 agcgccgtgt cgggatggcg cagcagctgt tgcgtcacct ggtggtcgcg atcgtcggcg
     7741 accatggtca gcaggttcgg atcgaagccc gcctcgacga gcagctcgcg cgcgctcctg
     7801 aggaacagcg ccaccggcag gatcgcattc gggtgcggct tcaggatcac cgggttgccg
     7861 gtggcgaggt tcgccatcac ggccgggtag ccgttccact gcgggaaggt cgcgcaggtg
     7921 atcaccacgg ccggcccgcg cggcaggatg cggtaatgct tctcgagccg cgcattttcc
     7981 ggcccgccga agccgcgctc ccactgcgcg ctctgcggca cgtcgcgcat ggccttccac
     8041 gcgtaggcca gcgcctcgag gccgcgatcg agcgcattcg cgccgcagcc ggcgaaggcc
     8101 atgatgtagc tctgccccgc ggtgtgcatg gtcgcgtgcg cgttcacgaa cagcgccagc
     8161 ggatctcccg cgcgcgcgag catctcgagg cagatgccga tgcgctcgtt catcgcggcg
     8221 tcgcgccatg ccggccacgc gcgccgcgcc gcggcgaacg cggtatcgac gtccaggcgc
     8281 ggataacgga tgccgagcgc ctcgcgactg aacggcgaga cctcgccgcc cacccagccg
     8341 tcgtgtccgg gcagcagccc tatcccgaag cgctcgccga gcagcgcctc gaactcgcgc
     8401 ctgccgcgcg cgggcagttc cgtgccgtgc acacgactgc tcggcgactc cggatacgcg
     8461 gtccaggaat agcggcgcgc acaggcatcg agtgccttgt cgagggtggc gacgtgcttc
     8521 tcaaaccagg aactcatcgt ctacgctgtg cccggatcgc ggtttttccg gattctactc
     8581 gctttcatta aaagaccaat agcgccgcgc gcacgcgacg ctcacggtcg caacgcagcc
     8641 cggagcagcc cgatgaacgc ggcgcacatc ctgccctcca gcccgatcgc caaggcgctc
     8701 gcggtggtcg gcgaccgctg gagcgcgctg gtgatgcgcg agatgttcct gggcaccaca
     8761 cgcttcggcg agctcgccgc ggccaccggc gcgagccgcg ccacgctgag caagcgcctg
     8821 cgcgcactcc tcgccaacgg cctgctctac cagaacccct accagatgcg cccgcagcgg
     8881 gtggagtacc gcctcaccga aaagggcttc ggactctacg atttcgcgct cgcgatctgg
     8941 acctgggaag cccgctggag ccggggcgcc gaccgccgcc tgccgcccag gctgctgcac
     9001 cgcggctgcg gccacaagac cctgcccacg gcctgctgcg cgcgctgcgg caagcccatc
     9061 gatatccacg agctgcgcta cgaggccggg atggcgcgca gcgcggagag cgcggtcaac
     9121 gacggtgcgc tgcgccgctc gaccagggcg ctgcccgagg ccggcgatgg caagggactg
     9181 ctgctgcatg ccatcgacct gatcgccgat cactggacac cgctggtgct ggcggcagcc
     9241 ttcatgggca tccgccgcta cgacgacatc cgccgcgaac tcggcatcgc gaccaacatc
     9301 ctctcgcacc gcctcaagct gctggtggag gccggggtgc tgcagcaggc gcccggcacg
     9361 ctctcgggca agcgcctgga gtaccgcctg agcgaaaagg ggcgcgcgct gatcgtgccc
     9421 gcgctcgcgc tgcaccagtg gagcctgaaa tggctgcccg ccggcaaggg
//
LOCUS       DAORUQ010000124         9441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7808_length_9441_cov_5.621457, whole genome shotgun sequence.
ACCESSION   DAORUQ010000124 DAORUQ010000000
VERSION     DAORUQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7808_length_9441_cov_5.621457"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLW13_11105"
     CDS             <1..1307
                     /locus_tag="PLW13_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQY70968.1"
                     /translation="GRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLVVSGCIMMR
                     KCHLNTCPVGVATQDPVLRRKFAGKPEHVVNFFFFIAEEVRQIMAQLGIRRFDELIGR
                     SDLLDTKKGIEHWKARGLDFSRVFYRPDVPEDVARRQVEEQDHGLERALDASLIARCA
                     PALERGERVQILDDVRNVNRTVGAMLSGELIRRHPEGLPDQTVWVQLNGTGGQSFGAF
                     LARGITLYLAGEANDYTGKGLSGGRVVIRPSIDFRGDSTENIIVGNTVLYGATSGEAF
                     FRGVAGERFAVRLSGATAVVEGTGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVY
                     DEDGEFASRCNTAMVALEDVLPAREQKASVDPAIWHKGQVDEELLRGLIEQHHRWTGS
                     LRARELLDDWDEARERFVKVFPHEYKRALAELNAAVPAAASA"
     gene            1336..2802
                     /locus_tag="PLW13_11110"
     CDS             1336..2802
                     /locus_tag="PLW13_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HQY70969.1"
                     /translation="MGKVTGFLEYERLEEGYEPVEARLKNYREFVKVLDVGQAQQQGA
                     RCMDCGIPFCNNGCPVNNVIPDFNDLVYRGDWHNALAVLHSTNNFPEVTGRICPAPCE
                     AACTLNFNNVPVGIKSIEHAIIDRGWDEGWVLPQIPAVKTGKRVAVVGSGPAGLAAAQ
                     QLARAGHDVTVFEKNDRVGGLMRYGIPDFKLDKSHIDRRMAQMNAEGVSFRTGVLVGS
                     MPEGSKVTNLSQQTVAPEELLNDFDAVLLAGGAEQPRDLPAPGRELDGIHFAMEFLPQ
                     QNRVNAGDKVKKQISAAGKHVIVIGGGDTGSDCVGTSNRHGAASVTQFELLPMPPEQE
                     NKALTWPYWPIKLRTSSSHVEGCERVFAISTREYIGEKGKVTAIRTVNVEMKDGKFIE
                     IEGSEQVLKADLVLLAMGFLSPVGTVLDAFGVAKDPRENARAATEGAEAYRTNVDKVF
                     AAGDMRRGQSLVVWAIREGRQAARAVDEYLMGSSTLPR"
     gene            complement(2942..3349)
                     /locus_tag="PLW13_11115"
     CDS             complement(2942..3349)
                     /locus_tag="PLW13_11115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY70970.1"
                     /translation="MATVERINPPGLFELEGFSQVVVARGGRTAYIAGQGPFDAGMNL
                     IGRGDLHAQTVQAFRNLRTALQALGVGPERVVSSTMYVVGLDQERSATFVRAMNVALD
                     GEPFPPNASSLIGVARLGHPEMLVEISAIAVLD"
     gene            complement(3420..4298)
                     /locus_tag="PLW13_11120"
     CDS             complement(3420..4298)
                     /locus_tag="PLW13_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQY70971.1"
                     /translation="MAVWRYPVKSMAGEAVAQALVDARGLAGDRHWAVIDGDAREIRS
                     AKRWPELLGCVARYVGGSTPATDAYAAAVLPVEIVGPDGRILHSDAADRDAELSAWLQ
                     RAVRLSPRAPAHDRDHYRLARSRTPESTAAEMDLQAHEAPPDFSLLSDAAAVFMQDLA
                     DCATPPGSYVDAFPLHLMSRNALAWLRARSGLDTDVRRFRPNLLIDIDGAGAEPGEDA
                     WIGWHLAIGEARLRIDSRTVRCAMPGRAQPQFGLAAQPTLARALVEHSARQLGVNVIV
                     EQAGVIRAGDAIERVG"
     gene            complement(4496..6493)
                     /locus_tag="PLW13_11125"
     CDS             complement(4496..6493)
                     /locus_tag="PLW13_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQY70972.1"
                     /translation="MRTDGPDGQLADFEVKYTFGLEPLQQYLVELPGGRLQALALGWD
                     TRPASAGGQRWFRQYPDEHIDYRDELHWTGRAQNWNFMCADCHSTDVRKGFDADAFAT
                     RWSEISVGCEACHGPGSAHLSWSKAPDASDPSKGLTVALDERRGAHWSIDPASGNAQR
                     STPRDSERELQVCAQCHSRRSQFAEGYRAGRPFHDHYLPSTLEEGLYHPDGQQRDEVF
                     TWGSFRQSRMHQAGVTCGDCHEPHGQKLRAEGNAVCGQCHAAAKYDAPSHHFHPVGSP
                     GAQCVNCHMPATTYMVVDPRRDHSFRVPRPELSMALGTPNACTGCHRDQQAQWAADAV
                     AKWYGPGRRQEAHFGAAIDAGRRAGPGAAAALLALVRDSERPAIVRATAIELLGRYPG
                     AAAHDAIRHALRDTDAQVRHAAVTAQQAAAPEHIALLLAPLLGDPTRAVRMEAARVIA
                     AAGALPAPMAMAAFNAAIAEFESAQRENLDRPEAWLNLGNLAGWRGDAAGAEEAYRRA
                     LQRDPRFVPAAINLADLYRATGREAQGEQVLRDGLQAALQAPALHEALGLALVRQGRK
                     TEALVELATAHRAAPDEPRHAYVYAVALADAGQRSEAIRVLEENARRAGGSRDVLLAL
                     AAFRSDSGDAAGAGQALQSLAAVNPDDPALAATRLPQQPGP"
     gene            complement(6448..6945)
                     /locus_tag="PLW13_11130"
     CDS             complement(6448..6945)
                     /locus_tag="PLW13_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQY70973.1"
                     /translation="MTRKRKPRSVPAPALAAGTRPAISATTAHRRWWPGLLAALAALG
                     IAAWLLSPRDAPPAAPAPATEAAATGAPTPPSRPAGASAAPPEVAGFVGSEACGECHR
                     AEHAAWQGSQHARAMQHATPATVLVISMTPASTSRASSRASSGATAASSCVPTDPTAS
                     SPTSR"
     gene            7067..9217
                     /locus_tag="PLW13_11135"
     CDS             7067..9217
                     /locus_tag="PLW13_11135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQY70974.1"
                     /translation="MAYEVMMAAMTQPILPFAATSLAASAANGPASLNRAQREAVAHG
                     PGAAVPAGPLLVIAGAGSGKTNTLAHRVARLIADGADPGRILLLTFTRRAAEEMGRRV
                     RRILAEAATARPALARAQLSWSGTFHGIGARLLRDYAGRIGLDPGFTIHDREDSADLL
                     NLVRHELGLSSTRRRFPQKGTCLAIYSAAVNTRAPIGEVLQSAFPWCAEWAEELKGLF
                     RAFVEAKQAQQVLDYDDLLLYWAQMVAEPALGAEIGGLFDHVLVDEYQDTNHLQAAIL
                     LAMKPDGRGLTVVGDDAQSIYAFRGATVRNILDFPAAFTPPATVVTLEQNYRSTRPIL
                     EAANAVIALAAERFTKNLWSERDSTARPRLVSVKDEADQAAFVADTALARREEGLRLR
                     QQAVLFRAGAHSARLELELARRNIPFVKYGGLKFLEAAHIKDVLALLRWAENPRDRVS
                     GFRAIQLLAGMGPKSAARVMDNTLAAGGALRDAAGVPPLAREGWCGFAALIDRLAGRA
                     DPPSVLTPQWPADFELACRWYEGQMPRLYEDAAVRLLDIRQLARIAASYPDRERFLTE
                     LTLDPPSATSDEAGVPLADEDYLILSTMHSAKGQEWNAVSVLNVVDGCIPSDIATRNR
                     AEIEEELRLLYVAMTRAKDTLDLVVPQRFYVTQQSGTGDRHVYAGRSRFIPNALLRYF
                     EETGWPPAPAVAEPDQASRQMAVDLAAKMREMWR"
BASE COUNT         1410 a         3276 c         3374 g         1381 t
ORIGIN      
        1 gcgggcgcat ccgcgtgcag gccgacggcc agatgaagac cgggcgcgac gtggtgatcg
       61 gcgcgctgct cggggccgac gagttcggct ttgccaccgc gccgctggtg gtgagcggct
      121 gcatcatgat gcgcaagtgc cacctgaaca cctgccccgt gggcgtggcg acgcaggacc
      181 cggtgctgcg ccggaagttc gccggcaagc ccgagcacgt ggtgaatttc ttcttcttta
      241 tcgccgagga agtccgccag atcatggcgc agctgggcat ccgccgcttc gacgagctga
      301 tcgggcgctc ggatctgctc gacaccaaga agggcatcga gcactggaag gcgcgcggcc
      361 tggatttcag ccgcgtgttc taccgccccg acgtgcccga agacgtggcg cgccgccagg
      421 tagaggagca ggaccacggg ctcgagcgcg cgctcgacgc ctcgctgatc gcgcgctgcg
      481 cgccggcgct cgagcgcggc gagcgggtgc agatcctcga cgacgtgcgc aacgtcaacc
      541 gcaccgtggg cgctatgctc tcgggcgagt tgatccgccg ccaccccgag ggattgcccg
      601 accagaccgt gtgggtgcag ctcaacggca cgggcggtca gagcttcggc gccttcctcg
      661 ccaggggcat cacgctgtac ctggccggtg aagccaacga ctacacgggc aaggggctct
      721 cgggcgggcg cgtggtgatc cgcccgagca tcgattttcg cggcgactcg accgagaaca
      781 tcatcgtggg caacacggtg ctctacggcg cgaccagcgg cgaggccttc ttccgcggtg
      841 tcgcgggcga acgcttcgcg gtgcggctct cgggcgcgac tgccgtcgtc gagggcaccg
      901 gcgatcacgg ctgcgagtac atgaccggcg gcaccgtggt ggtgctcggc aagaccgggc
      961 gcaacttcgc ggccggcatg tcgggcggcg tggcctacgt ctacgacgag gacggcgagt
     1021 tcgcgagccg ctgcaacacc gcgatggtgg cgctcgagga cgtgctgccg gcgcgcgagc
     1081 agaaggcgag cgtcgacccg gcgatctggc acaagggcca ggtcgacgag gagttgctgc
     1141 gcgggctgat cgagcagcac caccgctgga ccggctcgct gcgcgcgcgc gaactgctgg
     1201 acgactggga cgaggcccgc gagcgcttcg tgaaggtgtt cccgcacgaa tacaagcgcg
     1261 cgctcgccga gctgaacgcg gcagtgccgg ccgcggccag cgcctgacca ccacttcgcc
     1321 tggagtacaa ggatcatggg taaagtcacg ggttttctcg aatatgagcg cctcgaggaa
     1381 ggttacgagc cggtcgaggc gcggctgaag aactaccgcg agttcgtgaa ggtgctggac
     1441 gtcgggcagg cgcagcagca gggcgcgcgc tgcatggatt gcggcatccc gttctgcaac
     1501 aacggctgtc cggtcaataa cgtgatcccg gacttcaacg acctggtgta tcgcggcgac
     1561 tggcacaacg ccctggccgt gctgcattcg accaacaact tccccgaggt gaccggccgc
     1621 atctgcccgg cgccctgcga ggcggcgtgc acgctgaact tcaacaacgt gccggtgggc
     1681 atcaagtcga tcgagcacgc catcatcgat cgtggctggg acgagggctg ggtgctgccg
     1741 cagatccccg ccgtgaagac cggcaagcgc gtggccgtcg tgggctcggg cccggcggga
     1801 ctcgccgccg cgcagcaact ggcgcgcgcc gggcacgacg tcaccgtgtt cgagaagaac
     1861 gatcgcgtcg gcggcctgat gcgctacggc atccccgact tcaagctcga caagtcgcac
     1921 atcgacaggc gcatggcgca gatgaacgcc gagggcgtga gctttcgcac cggggtgctg
     1981 gtcggcagca tgcccgaggg cagcaaggtc accaacctgt cgcagcagac cgtcgcgccc
     2041 gaggaactgc tgaacgactt cgacgccgtg ctgctggctg gcggcgcgga gcagccgcgc
     2101 gacttgcccg cgccggggcg cgagctcgac ggcatccact tcgcgatgga gttcctgccg
     2161 cagcagaacc gcgtcaacgc gggcgacaag gtgaagaagc agatctcggc cgcgggcaaa
     2221 cacgtgatcg tgatcggcgg cggcgacacc ggcagtgact gtgtcggcac ctcgaaccgt
     2281 cacggcgcgg ccagcgtcac gcagttcgag ctgctgccga tgccgcccga gcaggagaac
     2341 aaggcactga cctggccgta ctggcccatc aagctgcgca cctcctcgag ccacgtggaa
     2401 ggctgcgagc gcgtgttcgc gatctccacc agggagtaca tcggcgagaa gggcaaggtc
     2461 acggcgatcc gcaccgtcaa cgtcgagatg aaggacggca agttcatcga gatcgagggc
     2521 agcgagcagg tgctgaaggc cgacctggtg ctgctggcga tgggcttcct gagccccgtc
     2581 ggcaccgtgc tcgatgcctt cggcgtggcg aaggacccgc gcgagaacgc gcgcgccgcc
     2641 acggaaggtg ccgaggccta ccgcaccaac gtcgacaagg tgttcgcggc cggcgacatg
     2701 cgtcgcggcc agtcgctggt ggtgtgggcg atccgcgagg gccgccaggc cgcgcgcgcg
     2761 gtcgatgagt acctgatggg ctcgagcacg ctgccgcgct gatgcgggtc ggcattcatg
     2821 ttgtaggtcg ccattcatgg cgacctacgg ccatgagtgg cgacctacgg cgatgaatga
     2881 cgcccggcct gccggtcaat agccgaccgt caggcgcatc acgatcagtt gagcgctcga
     2941 ctcagtcgag cacggcgatc gcggagattt ccaccagcat ctcggggtgg cccagccgcg
     3001 ccacgccgat caggctcgaa gcgttcggcg ggaacggttc gccgtcgagc gcgacgttca
     3061 tcgcgcgcac gaacgtcgcg cttcgctcct gatcgagacc gaccacgtac atcgtcgacg
     3121 acaccacgcg ctccggcccg acgccgagcg cttgcagtgc ggtgcgcagg ttgcggaagg
     3181 cctgcaccgt ctgggcgtgc agatcacctc gcccgatcag gttcatgccg gcatcgaagg
     3241 gcccctggcc cgcgatgtag gcggtgcggc cgccccgggc gactaccacc tgggaaaagc
     3301 cttcgagctc gaacaggccg ggaggattga tgcgttctac cgtcgccatc gcaggcactc
     3361 cattttgtga ttgttgtgaa ccgcacgcgc atgcgctcgc ccggcgatgg gcggctcgct
     3421 cagccgacgc gctcgatcgc atctccggcc cggatcacgc cggcctgttc cacgatcacg
     3481 ttgacgccga gctgccgcgc actgtgctca accagcgccc gtgccagcgt cggctgcgcc
     3541 gccagcccga attgcggctg cgcccgcccc ggcatggcgc agcgcaccgt gcgcgagtcg
     3601 atgcgcagac gcgcttcgcc gatggccaga tgccagccga tccaggcatc ctcgcccggc
     3661 tccgcgccgg caccgtcgat atcgatcaac agattcggac gaaaccggcg tacgtcggtg
     3721 tcgaggccgc tacgagcgcg cagccacgcc agcgcattgc gactcatcag atgaagaggg
     3781 aaggcatcga cataactgcc cggcggcgtc gcgcagtcgg cgagatcctg catgaagaca
     3841 gcggcggcgt ccgacaggag gctgaaatcc ggtggtgcct cgtgcgcctg cagatccatc
     3901 tcggcggccg tcgattccgg cgtgcgcgag cgcgccagcc ggtagtgatc gcgatcgtgt
     3961 gcgggcgcgc gcgggctcag ccgcaccgcg cgctgcagcc acgcgctgag ttcggcgtcg
     4021 cggtccgctg cgtcgctgtg caggatgcgg ccgtccggcc ccacgatttc caccgggagc
     4081 actgccgcgg cataggcgtc ggtcgcgggc gtgctccccc cgacgtagcg cgcgacgcag
     4141 cccagcagct caggccagcg cttggcgctg cgaatctccc gggcgtcgcc gtcgatcacc
     4201 gcccagtgac gatcgcccgc caggccccgg gcatccacca gcgcttgcgc caccgcttcg
     4261 cccgccatgc tcttcaccgg atagcgccag accgccacca ccttacctac ttgcatatcg
     4321 catcctcgag ccgagccgcc gccccggttc cagccgccac catacgatat tgcgcacgga
     4381 agcaggaacc cggcagggcg cggagtgaat gagcaggccc tcgcgggtcg gcattcgtgc
     4441 cgaaaaaggg ttcgtcggcg gcacctgtcg ccatgaatgg cgacccgcgt tgcggtcagg
     4501 gtcccggctg ttgcggtaac cgtgtggcgg cgagcgccgg gtcgtccgga ttgactgccg
     4561 ccagtgactg cagcgcctgc ccggcgcccg ccgcgtcgcc gctgtcgctg cgaaacgccg
     4621 ccagggccag tagcacgtcg cgcgagcctc cggcgcggcg cgcgttctcc tcgagcaccc
     4681 ggatcgcctc gctgcgttgc cccgcatcgg cgagcgcgac ggcgtacaca taagcgtggc
     4741 gcggctcgtc cggtgcggcg cggtgcgcgg tcgccagttc gaccagggct tcggtcttcc
     4801 tgccctggcg caccagtgcc aggccgagcg cctcgtgcag ggccggtgct tgcagtgccg
     4861 cctgcagacc gtcgcgcagg acctgttccc cctgcgcttc ccgcccggtc gcccggtaca
     4921 gatcggcgag gttgatcgcg gccggcacga agcgcggatc gcgttgcagc gcgcgccggt
     4981 aggcctcctc ggcgcccgcg gcgtcgccgc gccagccggc caggttgccg aggttcagcc
     5041 acgcttcggg gcggtcgagg ttctcgcgtt gcgcggattc gaattccgcg atcgccgcat
     5101 tgaatgcggc catggccatc ggggccggca gggcgccggc agcggcgatc acgcgcgcgg
     5161 cttccatgcg caccgcgcgc gtcggatcgc cgagcagcgg cgcgagcagg agcgcgatat
     5221 gttccggcgc tgccgcctgc tgtgcggtga ccgcggcgtg acgcacctgc gcatcggtat
     5281 cgcgcagcgc gtgtcggatc gcgtcgtgcg cggccgcgcc ggggtagcga ccgagcaact
     5341 cgatcgccgt ggcgcgcacg atcgccgggc gctcggaatc ccgcacgagg gcgagcagcg
     5401 cggccgcggc gcccggcccg gcacgccgac cggcgtcgat ggccgcgccg aaatgtgcct
     5461 cctgccgccg ccccggccca taccacttcg ccaccgcatc cgcagcccat tgcgcttgct
     5521 ggtcgcggtg gcagccggtg caggcattcg gcgtgcccag cgccatcgac agctcgggac
     5581 gcggcacacg gaagctgtgg tcacggcgcg ggtccacgac catgtaggtc gtcgccggca
     5641 tgtggcagtt cacgcactgg gcgcccggcg agcctaccgg gtggaagtgg tgcgacgggg
     5701 cgtcgtattt cgccgcggcg tgacattggc cgcagacggc gttgccctcg gcgcgcagct
     5761 tctgtccgtg cggctcgtgg cagtcgccgc aggtgacgcc ggcctggtgc atccggctct
     5821 ggcggaacga accccaggtg aatacctcgt cgcgctgctg gccgtcgggg tggtagaggc
     5881 cctcctccag agtggaaggc aggtaatggt cgtggaacgg ccggccggcg cgatagccct
     5941 cggcgaactg cgaacggcgc gagtggcact gcgcgcacac ctgcaactcg cgctcgctgt
     6001 cgcgcggcgt gctgcgctgc gcgttgccgc tggccgggtc gatcgaccag tgcgcgccgc
     6061 ggcgctcgtc cagtgccacc gtcaggccct tcgacggatc ggatgcatcc ggggccttgc
     6121 tccatgacag gtgcgctgat cccggtccgt ggcaggcctc gcagcccacg ctgatttccg
     6181 accagcgggt agcgaaggcg tcggcgtcga agcccttgcg cacgtcggtc gaatggcagt
     6241 cggcgcacat gaagttccag ttctgcgcgc gccccgtcca gtgcagctca tcgcggtaat
     6301 cgatgtgctc gtccgggtac tggcggaacc agcgttgtcc gccggcgctc gccgggcgcg
     6361 tgtcccagcc cagtgccagg gcctgcaggc gcccacccgg cagttcgacg aggtattgct
     6421 gcagcggctc gaggccaaag gtgtacttca cctcgaagtc ggcgagctgg ccgtcgggtc
     6481 cgtcggtacg cacgaagaag cggccgtcgc gccggaagaa gcgcgactcg acgccctgga
     6541 cgttgaggcg ggcgtcatcg aaatcaccag caccgtggcg ggcgtggcgt gctgcatcgc
     6601 gcgcgcgtgc tgcgaaccct gccaggccgc atgctcggca cgatgacatt cgccgcaggc
     6661 ctcgctgccg acaaatccgg cgacctcggg cggggcggca ctggccccag ccggacggga
     6721 tggcggcgtc ggggcgccgg tcgcggcggc ttcggttgcc ggcgccgggg cggctggcgg
     6781 tgcgtcgcgc ggcgaaagca accacgcggc gatgccgagc gcggccagcg cggcgagcag
     6841 tcccggccac caccgacggt gcgcggtcgt ggctgagatc gcggggcgcg tgccggcagc
     6901 caacgcgggg gcgggtacgg atcggggctt gcgcttcctg gtcatgcgag gcatcggttg
     6961 tgggcggtcg tcagcgcagc cgcggcgccg gcgcgcactt tagcagatgc catcaggaat
     7021 gccggcaggc gagcgcgagg ggcggacgcg cgacagggac cggggcgtgg catacgaggt
     7081 tatgatggcc gccatgaccc agccgatcct cccgttcgct gccacctcgc tcgctgcgtc
     7141 cgccgccaac gggccggcca gcctcaatcg cgcccagcgc gaggccgtag cgcacggtcc
     7201 cggcgccgcc gtgcccgccg gtccgctgct ggtgatcgcc ggcgcgggct cgggcaagac
     7261 caacaccctg gcgcatcgcg tcgcgcgcct gatcgccgat ggcgccgacc ccgggcgcat
     7321 cctgctgctc accttcacgc gccgtgccgc cgaggagatg gggcggcgcg tgcgacgcat
     7381 cctggccgag gccgcgacgg cccgcccggc gctggcgcgc gcgcaactgt cgtggtcggg
     7441 caccttccac ggcatcggcg cgcggctgct gcgcgactat gccgggcgca tcggtctcga
     7501 tccgggcttc accatccacg accgcgagga ttcggcggac ctgctgaacc tggtgcgcca
     7561 cgagctcggg ctgtcgtcca cgcgccggcg cttcccgcag aagggcacct gcctggcgat
     7621 ctattccgcg gcggtgaaca cgcgcgcgcc gatcggtgag gtgttgcagt cggcctttcc
     7681 gtggtgcgcg gagtgggcgg aggaactgaa gggcctgttc cgcgccttcg tcgaggccaa
     7741 gcaggcgcag caggtgctcg attacgacga cctgctgctc tactgggcgc agatggtggc
     7801 cgagcccgcg ctcggcgccg agatcggcgg cctgttcgac cacgtgctgg tcgacgagta
     7861 ccaggacacc aaccacctgc aggccgcgat cctgctggcg atgaagcccg acggtcgcgg
     7921 cctcaccgtg gtgggcgacg acgcgcagtc gatctacgcc ttccgcggcg ccacggtgcg
     7981 caacatcctc gattttccgg ccgccttcac gccgccggcg accgtggtga cgctggagca
     8041 gaattaccgc tccacgcgcc ccatcctcga ggccgcgaac gcggtgatcg cgctcgccgc
     8101 cgagcgtttc acgaagaacc tgtggtcgga gcgcgactcc accgcgcgcc cgcgcctggt
     8161 atccgtgaag gacgaggccg accaggccgc cttcgtcgcc gacaccgcgc tggcgcggcg
     8221 cgaggaaggg ctgcggctgc gccagcaggc ggtgctgttt cgcgccggcg cgcacagcgc
     8281 ccgcctcgag ctggagctcg cgcggcgcaa tatcccgttc gtgaagtacg gcgggctgaa
     8341 gttcctcgag gcggcgcaca tcaaggacgt gctggcgctg ctgcgctggg ccgagaaccc
     8401 gcgcgaccgc gtgtccggtt ttcgcgccat ccagttgctg gcgggcatgg gtccgaagtc
     8461 ggctgctcgc gtgatggaca ataccctggc cgcgggcggg gcgctgcgcg atgccgccgg
     8521 cgtgccgccg ctggcgcgcg aaggctggtg cggtttcgcg gcgctgatcg atcgcctcgc
     8581 gggccgcgcc gatccgccgt ccgtcctgac tccgcaatgg cccgcggatt tcgagctcgc
     8641 ctgccgctgg tacgagggac agatgccgcg cctctacgag gacgccgcgg tacggctgct
     8701 cgacatccgc cagctcgcac gcatcgcggc gagctacccg gaccgcgagc gtttcctcac
     8761 cgagctcacg ctggatccgc ccagcgccac cagcgacgag gccggcgtgc cgctcgcgga
     8821 cgaggactac ctgatcctgt cgacgatgca ctcggccaag ggccaggagt ggaacgcggt
     8881 cagcgtgctc aatgtggtcg acggctgcat tccttccgac atcgccacgc gcaaccgcgc
     8941 cgagatcgag gaggagctgc gcctgctgta cgtggcgatg acgcgcgcga aggacacgct
     9001 cgacctggtc gtgccgcagc gcttctacgt cacgcagcag tccggcacgg gggacaggca
     9061 cgtctacgcc gggcgcagcc gcttcatccc gaacgcgctg ctgcgttact tcgaggagac
     9121 cggctggccg ccggcgccgg cggtcgcaga accagatcag gcctcgcggc agatggcggt
     9181 ggacctcgca gcgaagatgc gcgagatgtg gcgctgaaac accgagcgct gacggcggcc
     9241 tcgggtcgac gacaggcgct ggtcgcgggg ctatcccgcc aacgccccgg ccagcaatgg
     9301 cgcgagatcg atggcgttgc tggcgtgcgg gatcgaattc gtcgtgacga tgcgtccggc
     9361 gccggcggcg agcagggctt cgtaggcatc cccggcgaag ataccgtgca ccgcgagcac
     9421 cagcggcgcg cgcatgccgg c
//
LOCUS       DAORUQ010000125         9375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7903_length_9375_cov_5.988412, whole genome shotgun sequence.
ACCESSION   DAORUQ010000125 DAORUQ010000000
VERSION     DAORUQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7903_length_9375_cov_5.988412"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1836)
                     /gene="recD"
                     /locus_tag="PLW13_11140"
     CDS             complement(4..1836)
                     /gene="recD"
                     /locus_tag="PLW13_11140"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HQY70975.1"
                     /translation="MSLEALTALHGAGLLRPVDLHFARLMAGYADSGHEAIALIAALL
                     SVELGRGHVCVELAAVDGKPFAGLESPLAPRLDAAQLRAALVAEARLCAGPASTAPLP
                     LVLDGDCVYLHRYWAGEGRVAARLRALATRPVAAAPDARALLDRLYPDEAAGSAGQKL
                     ACAMALTRALGVISGGPGTGKTTTVARLLVMLAAAAAAEGRSLAIRLAAPTGKAAARV
                     GEALAREVAALARGGQVAPQLLAQLPTSAQTLHRLLGAGSRGLRHDAANPLAADVVVL
                     DESSMVDLRLLDALLAALPRHARLLLLGDRDQLAAVEAGNVFGALCSRARGPSAQRAA
                     ELTALTGLPIAGNDHDDPIADAIAVLRHSYRFDPASGIGRFAEAVNAGDATRAAAVLA
                     QRPGDLGSICCTERPDGAAITRMREGWRDIASAARGHADPATLVRLQESFRVLCALRE
                     GEWGVAGVNRALTQTLAEAGLAERGRTHYAGRPLLVTRNDYGLGLYNGDMGIVIEDED
                     GHPQALFAQAGGGMKRLPAARLPEHETAYALTVHKSQGSEFDEVVLVLPPAPASGAGI
                     AGRELLYTAITRARRRLELVLPGGTLDARWLRASEYRSGLAERL"
     gene            complement(1826..5344)
                     /gene="recB"
                     /locus_tag="PLW13_11145"
     CDS             complement(1826..5344)
                     /gene="recB"
                     /locus_tag="PLW13_11145"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HQY70976.1"
                     /translation="MIRLDPCTLPLAGRCLIEASAGTGKTYTITSIVLRLLLGHAGAD
                     GPPVPPRRVQEILIVTFTKAATEELRGRIRKRLRAAREAFETGAPPPADTVIAALLAA
                     SEDRAEDSRRLRLAEMELDLASIFTIHGFANRMLKRNAFESRISFAAELSESDAAVVT
                     EAIRDIWRERAYPLPPLPASLLLAEHDCDGFVAAVRRLLGKQGLELRNVPEQDWDGVL
                     AQMAAAESALLAWWRASAATAREALAGTALNKGGRTELAAALPRLDAAAAGHATLDRR
                     TLAGVSRTFLSGELTKRKGNLLPELALFDLAETLLVAARALWPRLLADALAIARERLA
                     AIKERTGLLGYEDLLRLLDEGLRAPGGERLAAAIRSQYPVALIDEFQDTDPLQWSVFS
                     AVYAGDDTALFLVGDPKQAIYAFRGADVQAYLQVRREAAGTIRSLDTNYRSVQPLVEA
                     VNALFRLRSGGDPFLAGDDMPFQAVLASGAPDKAPLRRHGQPVTPLAVLHLASAQPLS
                     GTAYRARMAAAAANHLAGLLGAADSGGVQLGDEPLAPGHIACLVRDWQDAKRLAAELD
                     RRGVPHVYRSRDSVYAGPQALDLYQLLAAVLEPGSERLLRAALGSALLGRTAAELDAL
                     RSDEAAFAREQGQFLGFSEELRLHGVQAMLRRLLFAYEVPARLLATPAGERALTDCLH
                     LVELLQAEREGLDSDEALLARLAERIAGADGEQEAQQLRLESDAKRVQIVTLHSSKGL
                     EYPVVYLPFMPIFKPAQDAFFHDPDTLVACYDLQGTEASRALAERERLAEDMRLLYVG
                     LTRAAQLCVLGIGDTKRGNHSKSELWRSALGHLVGAGPGDGAAAAAIARLGTLPGTKL
                     IDVDALPFVTLARASAPVPALAARRFDTPIERDWQSSSYSALTRHADARAAQLFRQPE
                     LAADASAAQARERSLFGFPRGARHGSFMHGLLERIDFSQRADAPATRQLIARALVREG
                     YEADWLPVFTAMIEDVLGCALDGARLRLGALAPQQRVVEMGFEFPLAELDCARLNALL
                     AAHDPLARAAPPLAFAATRGMLTGFIDLVFEHEGRFYVADYKSNHLGNELGHYEPARL
                     GASIAEHRYDLQYALYTVALTRLLRARLGAAYDYDTHVGGVFYLYLRGMRAAAGPAHG
                     VFHARPPRALVEALDALFAGDNRDVA"
     gene            complement(5341..8571)
                     /gene="recC"
                     /locus_tag="PLW13_11150"
     CDS             complement(5341..8571)
                     /gene="recC"
                     /locus_tag="PLW13_11150"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HQY70977.1"
                     /translation="MLYQYHGNDMEALRDAACTLLGAAPAPPLVAERLIVPNVGMAKW
                     LRQGIAARLGVAANLRNDSPAVFLNALAGAVLDDAGPAGATAWSKEQLAIRLMRVLPG
                     LLEEPAFAPVRRYLEAATPERRLHALCRRLATLFDQYLLYRPHWLLAWEAGLPAPDCP
                     LAGNEWQASLWRALVQQIGAEHPASTHGAARLERLVARLGTGAPLPCALPERVIGFGL
                     GALAPVFVEALAALATRTDVHLFQFNPCGEYWYDIVSERTRARWQLLAPQRAEHAETG
                     NPLLASWGTLGRNGLELLLDRDGGELRQCFREPVQEGVLGELQRDILWLETPAAPRLL
                     APQDHGLVFAEAHSELREVEALQDQLLHLLATLPGLQPRDITVMAPDIGAYGGLINAV
                     FAKSRQDPRHIPFSIADRDASAGNAVVQSFLHLLGLPESRFAASAVLALLGVPALGAR
                     FDIDAEVLESVRARVRDSGIRWGLDGRRGAPGAPGPMRNSWRFGLERMLLGVALDEGA
                     VFEGATPFEAGGQDSIEEIGRLAEFIERLAHYAGTLAAPRAMDDWIALLRQLIADFYA
                     DTPESAADLAELYAAIAALGARLDEAGYRDTLTREVLLDMLQQELADAEASHQFLRGG
                     INFCQLTPLRSIPFRVVCLLGMNAEAFPRNTLAPAFDLMAREPRHGDPSRRDDDRYLF
                     LEALLSARDCFYISYVARDERSNEARERAVPVSELRDYIDRHWAAAPDDSPASAALTR
                     RHRLKPFHPAYFEAGGTLFSYRHEWLPAARAGAAPLPFCPEALPGRAVEELALPELLR
                     FFRNPCQGFFNIRLGVRFGEAEEMATDNEPFALDGLTAWALRDEMLATALAGGDTEAC
                     AARQVAAGLLPHGQAGQFALQAQRARSAALVATAEDWARLEPASAELALDIAGTRLHG
                     TVANLRGGALRQLNASKASGAARLQFWITHLCCCAAQLVCAPSELQHEDERVELPLLA
                     PEAATAHLADLLALHAEGLRRPLPLFPKSAWAFVQLLQKHDTEHALQQARKSFEDGFQ
                     HEGEGSNAYIARAFADVEAALGEEFAALAQRVFVPLLAAEAAEGAP"
     gene            8722..9075
                     /locus_tag="PLW13_11155"
     CDS             8722..9075
                     /locus_tag="PLW13_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQY70978.1"
                     /translation="MSPHLSTIVLLLCSNVFMTFAWYAHLKNLADKPWIVAALLSWGV
                     ALFEYLFQVPANRIGHQVMSVGQLKILQEVITLLVFVPFSLFYLRERLTLDYLWAGLC
                     ILGAVFFMFRAKLGL"
BASE COUNT         1275 a         3366 c         3434 g         1300 t
ORIGIN      
        1 gacctacagg cgttcggcca gcccggaacg gtactcgctg gcgcgcagcc agcgcgcatc
       61 gagcgtgccg ccgggcagca cgagctcgag gcgccggcgc gcgcgcgtga tcgcggtgta
      121 gagcagctcg cgtccggcga tgccggcgcc ggacgcgggg gcgggcggga gcaccagcac
      181 gacctcgtcg aactccgagc cctggctctt gtgcacggtg agcgcatagg cggtctcgtg
      241 ctcgggcagg cgcgccgccg gcaggcgttt catcccgcca ccggcctgcg cgaacagcgc
      301 ctgcggatgc ccgtcctcgt cctcgatcac gatgcccatg tcgccgttgt agagccccag
      361 accgtagtcg ttgcgcgtca cgagcagcgg gcgcccggcg tagtgcgtgc gcccgcgttc
      421 cgcgaggccc gcctccgcca gcgtctgcgt gagcgcgcgg ttcacgccgg ccacgcccca
      481 ttcgccctcg cgcagcgcgc acagcacgcg gaaggactcc tgcaggcgca ccagcgtggc
      541 agggtcggcg tgcccgcgcg ccgcgctcgc gatgtcgcgc cagccctcgc gcatgcgcgt
      601 gatcgccgcg ccgtcgggcc gctccgtgca gcagatgctg ccgagatccc cggggcgctg
      661 cgcgagcacg gccgcagcgc gcgtcgcgtc gccggcattg acggcttcgg cgaagcgccc
      721 gatgccgctc gcggggtcga agcggtaact gtgacggagc accgcgatcg cgtcggcgat
      781 cgggtcgtcg tggtcgttgc ccgctatcgg caaaccggtg agcgcggtga gctccgccgc
      841 gcgctgcgcg ctcgggccgc gcgcgcggct gcacagcgcg ccgaacacgt tgccggcctc
      901 cacggccgcg agctggtcgc gatcgccgag tagcagcagg cgcgcgtggc gcggcagcgc
      961 ggcgagcagc gcatcgagca ggcgcaggtc caccatcgag gattcgtcca gcaccactac
     1021 gtcggccgcg agcgggttcg cggcgtcgtg gcgcaagccg cgcgagccgg caccgagcag
     1081 gcggtgcagg gtctgcgccg aggtgggcag ctgtgccagc agttgcggcg ccacctgccc
     1141 gccgcgcgcg agcgcggcga cttcgcgcgc cagcgcctcg cccacgcgcg ccgcggcctt
     1201 gccggtgggc gcggcgaggc ggatcgcaag actccgtccc tccgccgcgg ccgcggcggc
     1261 gagcatcacc agcagtctcg cgacggtggt ggtcttgccg gtgccggggc cgccgctgat
     1321 cacccccagc gcgcgcgtca gtgccatcgc gcaggcgagc ttctgcccgg cgctgcctgc
     1381 cgcctcatcc ggatacagcc gatccagcag ggcgcgcgcg tcgggtgccg ccgcgacggg
     1441 ccgggtcgcc agcgcgcgca gacgcgccgc cacgcggccc tcgccggccc agtagcggtg
     1501 caggtagacg caatcgccgt cgagcaccag cggcagtggc gcggtggacg ccggtccggc
     1561 gcacagccgc gcctcggcca ccagcgccgc gcgcagttgc gcggcatcga ggcgcggcgc
     1621 cagcggcgat tcgagcccgg cgaagggctt gccatcgacg gcggcgagct ccacgcagac
     1681 gtggccgcgt ccgagctcca cgctgagcag cgcggcaatc agtgcgatcg cctcgtgccc
     1741 gctgtcggca tagccggcca tcaggcgcgc aaaatgcagg tccaccggcc gcagcagccc
     1801 ggcgccgtgc agtgcggtga gcgcctcaag cgacatcgcg gttgtctccg gcaaacagcg
     1861 cgtcgagcgc ctcgaccagc gcgcgcggtg ggcgcgcgtg gaacacgccg tgcgcggggc
     1921 cggcggcggc gcgcatgccg cgcaggtaca ggtagaacac gccgccgacg tgcgtgtcgt
     1981 agtcgtaggc cgcgccgagc cgcgcgcgca gcaggcgcgt cagcgccacg gtgtagagcg
     2041 cgtactgcag gtcgtagcga tgctccgcga tcgaggcgcc gaggcgcgcc ggctcgtagt
     2101 gcccgagctc gttgcccaga tggttcgact tgtagtcggc cacgtaaaag cggccctcgt
     2161 gttcgaatac caggtcgatg aagcccgtga gcatgccgcg cgtcgcggcg aaggcgagcg
     2221 gcggcgcggc gcgcgccagc gggtcgtgcg cggcgagcag cgcgttcagg cgcgcgcaat
     2281 cgagctcggc cagcggaaac tcgaagccca tttccaccac gcgttgctgc ggtgcgagtg
     2341 cgccgaggcg cagcctcgcg ccgtccagcg cacagccgag cacgtcctcg atcattgccg
     2401 tgaacaccgg cagccagtcc gcctcgtagc cttcgcgcac cagggcgcgc gcgatcaact
     2461 gccgggtcgc gggcgcgtcg gcgcgctggc tgaaatcgat gcgttccagc agcccgtgca
     2521 tgaagctgcc gtggcgtgca ccgcgcggga agccgaacag gctgcgctcg cgcgcctgcg
     2581 cggcgctcgc gtccgcggcc agttccggct ggcggaacag ttgcgccgcg cgcgcgtcgg
     2641 cgtgacgcgt cagcgccgaa tagctgctcg attgccagtc gcgctcgatc ggggtgtcga
     2701 agcggcgcgc cgccagcgcg ggcacgggcg cgctcgcgcg cgccagcgtg acgaacggca
     2761 gtgcatcgac gtcgatcagc ttggtgcccg gcagcgtgcc caggcgcgcg atcgcggccg
     2821 cggcggcgcc gtccccgggt cctgccccga ccaggtggcc cagcgcgctg cgccacagct
     2881 cgctcttgct gtggttgccg cgcttcgtgt caccgatgcc cagcacgcac agctgcgccg
     2941 cgcgcgtgag gcccacgtag agcaggcgca tgtcctcggc caggcgttcg cgctcggcca
     3001 gcgccctgct cgcctcggtt ccctgcaggt cgtagcaggc gaccagcgtg tcgggatcgt
     3061 ggaagaacgc gtcctgcgcc ggcttgaaga tcggcatgaa cggcaggtac accaccgggt
     3121 attccagccc cttcgacgaa tgcagcgtga cgatctgcac gcgcttcgcg tcgctctcga
     3181 ggcgcagttg ttgcgcctcc tgctcgccgt cggccccggc gatgcgctcg gccagccgcg
     3241 cgagcagggc ctcgtcgctg tcgagccctt cgcgctcggc ctgcagcagt tcgaccaggt
     3301 gcaggcaatc ggtcagcgcg cgctcgcctg cgggcgtggc gagcaggcgc gccggcactt
     3361 cgtaggcgaa cagcagccgg cgcagcatgg cctgcacgcc gtgcaggcgc agttcctcgg
     3421 aaaagccgag gaattgtccc tgctcgcgcg cgaacgccgc ctcgtcgctg cgcaaggcgt
     3481 cgagctccgc ggcggtgcgt ccgagcagcg cgctgcccag cgcggcgcgc agcaaccgct
     3541 ccgaacccgg ctccagcacg gcggccagca gctggtacag gtcgagtgcc tgcgggccgg
     3601 cgtagacgct gtcgcgactg cgatacacgt gcggtacgcc gcgccggtcg agctcggcgg
     3661 cgaggcgttt cgcgtcctgc cagtcgcgta ccaggcaggc aatgtggccg ggcgccaggg
     3721 gctcgtcgcc cagctgcacg ccgccgctgt ccgcggcgcc cagcagcccg gccaggtggt
     3781 tcgccgctgc agccgccatg cgcgcgcggt aggcggtgcc cgagagcggc tgtgcgctcg
     3841 cgaggtgcag caccgccagc ggcgtgacgg gctggccgtg gcgcctgagc ggcgccttgt
     3901 cgggagcgcc cgaggcaagc accgcctgga agggcatgtc gtcgcctgcc aggaagggat
     3961 cgccgccgct gcgcagccgg aacagcgcgt tcacggcctc gaccagcggt tgcaccgagc
     4021 ggtagttggt gtccagcgag cggatcgtgc cggcggcctc gcgccgcacc tgcaggtagg
     4081 cctgcacgtc ggcgccgcgg aaggcgtaga tcgcctgctt ggggtcgccg accaggaaca
     4141 gcgcggtatc gtcgcccgcg tacaccgcgg agaacacgga ccactgcaac ggatcggtgt
     4201 cctggaattc gtcgatcagc gccaccgggt actggctgcg gattgccgcc gcgagccgct
     4261 cgccgcccgg tgcgcgcagc ccctcgtcca gcagccgcag caggtcctcg tagccgagca
     4321 acccggtgcg ctccttgatc gcggcgagcc gctcgcgcgc gatcgcgagt gcgtcggcga
     4381 gcaggcgtgg ccacagcgcc cgggcggcga ccagcagggt ctctgccagg tcgaacagcg
     4441 ccagctccgg cagcaggttg cctttccttt tcgtcagctc gccgctgagg aaggtgcgtg
     4501 agacgcccgc gagcgtgcgc cggtccagcg tggcatgtcc ggccgccgcg gcgtccaggc
     4561 gcggcagcgc cgccgcgagc tcggtgcgcc cgcccttgtt cagcgcggtg ccggcgagcg
     4621 cctcgcgcgc ggtcgcggcg ctggcgcgcc accaggccag caacgccgac tcggcggccg
     4681 ccatctgcgc cagcacgccg tcccagtcct gctccggcac gttgcgcaac tccagcccct
     4741 gttttccgag caggcggcgc acggcggcga cgaacccgtc gcagtcgtgc tccgccagca
     4801 gcagcgaggc cggcaacggc ggcagcggat aggcgcgctc gcgccagatg tcgcggatcg
     4861 cctcggtgac caccgccgcg tcgctctccg agagttccgc ggcgaaggag atgcggctct
     4921 cgaaggcgtt gcgtttcagc atgcggttgg caaagccgtg gatcgtgaag atgctcgcga
     4981 ggtcgagctc catttccgcg agccgcagcc gccggctgtc ctcggcgcgg tcctcgctcg
     5041 ccgcgagcag cgccgcgatc accgtgtcgg cgggcggcgg ggcgcccgtc tcgaaggcct
     5101 cgcgcgcggc gcgcaaccgc ttgcggatgc gcccgcgcag ttcctcggtg gcggccttgg
     5161 taaaggtcac gatcaggatc tcctgcacgc ggcgcggcgg caccgggggg ccgtccgcgc
     5221 cggcgtggcc cagcagcagg cgcagcacga tgctcgtgat cgtgtacgtc ttgccggtgc
     5281 cggcgctggc ttcgatcagg cagcgaccgg ccagcggcag cgtgcagggg tcgaggcgga
     5341 tcatggcgcg ccctccgcgg cttccgccgc aagcagcggc acgaacacgc gctgcgccag
     5401 tgcggcgaat tcctcgccga gcgcggcctc gacgtcggca aaggcgcggg cgatataggc
     5461 gttgctgccc tcgccctcgt gctggaagcc gtcctcgaag ctcttgcgcg cctgctgcag
     5521 cgcgtgttcc gtatcgtgct tttgcagcag ttgcacgaat gcccaggcgc tcttcgggaa
     5581 cagcggcagc ggacggcgca ggccctctgc gtgcagcgcg agcaggtcgg cgagatgcgc
     5641 ggtcgccgcc tccggcgcca gcaacggcag ttcgacgcgc tcgtcctcgt gctgcagttc
     5701 gctcggcgcg cagaccagct gtgccgcgca gcagcacagg tgcgtgatcc agaactgcag
     5761 ccgcgccgcg ccgctggcct tcgaggcgtt cagctggcgc agcgcgccgc cgcgcaggtt
     5821 cgccaccgtg ccgtgcagtc gcgtacccgc gatatccagc gcgagctccg cgctcgcggg
     5881 ctcgagcctt gcccagtcct cggcggtggc gaccagcgcc gcgctccgcg cgcgttgcgc
     5941 ctgcagcgcg aactgtccgg cctggccgtg cggcagcaag ccggccgcga cctggcgcgc
     6001 cgcgcaggct tcggtgtcgc cgccggcgag cgcggtggcg agcatttcgt cgcgcagcgc
     6061 ccaggccgtg agcccgtcga gcgcgaaggg ctcgttgtcg gtggccatct cctcggcttc
     6121 cccgaagcgc acgccgaggc ggatgttgaa gaacccctgg cagggattgc ggaagaagcg
     6181 cagcagttcg ggaagcgcga gctcctcgac ggcgcgcccg ggcaaggctt ccgggcagaa
     6241 gggcagcggc gcggccccgg cgcgcgcggc cggcagccat tcgtggcgat agctgaacag
     6301 cgtgccgccg gcttcgaaat acgccgggtg aaacggcttc aggcggtggc ggcgggtcag
     6361 cgccgccgag gccggggagt cgtccggcgc ggcggcccag tgccggtcga tgtagtcgcg
     6421 cagctccgag accggcacgg cccgctcgcg tgcctcgttg ctgcgctcgt cgcgcgccac
     6481 gtagctgatg tagaagcagt cgcgcgccga gagcagcgcc tcgaggaaca ggtagcggtc
     6541 gtcgtcgcgg cgtgacgggt ccccgtggcg cggctcgcgc gccatcaggt cgaaggccgg
     6601 tgcgagcgtg ttgcgcggga aggcctcggc gttcatgccg agcaggcaca cgacgcggaa
     6661 cgggatgctg cgcagcggcg tcagctggca gaagttgatg cccccgcgca ggaactggtg
     6721 cgaggcctcg gcgtcggcca gctcctgctg cagcatgtcg agcagcacct cgcgcgtgag
     6781 cgtgtcccgg tagccggctt catcgagccg tgcgccgagt gcggcgatcg ccgcgtacag
     6841 ctccgcgaga tccgcggcgc tctcgggcgt atcggcgtag aaatccgcga tcagctggcg
     6901 caacagcgcg atccagtcgt ccatcgcgcg cggtgcggcg agcgtgccgg cgtagtgggc
     6961 gagccgctcg atgaattccg ccaaccggcc gatctcctcg atgctgtcct ggccaccggc
     7021 ctcgaacggg gtggcgccct cgaataccgc gccttcgtcg agcgcgacgc cgagcagcat
     7081 gcgctcgagg ccgaagcgcc agctgttgcg catggggccg ggcgcaccgg gggcgccgcg
     7141 ccgcccatcg agcccccagc ggatgccgct gtcgcgcacg cgggcgcgca cggactcgag
     7201 cacctcggca tcgatgtcga agcgcgcgcc gagtgccggc acgcccagca gcgcgagcac
     7261 cgccgaggcg gcgaaacggc tttcgggcag cccgagcagg tgcaggaagc tctgcaccac
     7321 cgcgttcccg gcgctggcgt cgcggtcggc gatgctgaac gggatatggc gagggtcttg
     7381 ccgtgacttg gcgaagaccg cgttgatcag cccgccgtag gcgccgatgt cgggcgccat
     7441 caccgtgatg tcgcgcggct gcaagcccgg cagcgtggcc agcaggtgca gcagctggtc
     7501 ctgcagcgcc tcgacctcgc gcagctcgct gtgcgcctcg gcgaacacca gcccatggtc
     7561 ctgcggcgcg agcaggcgtg gcgcggccgg tgtttccagc cacaggatgt cgcgctgcag
     7621 ctcgccgagc acgccctcct gcacgggctc gcggaagcac tggcgcagct cgccgccgtc
     7681 gcgatcgagc agcaactcca ggccgttgcg ccccagcgtg ccccaggatg ccagcaacgg
     7741 gttgccggtc tccgcgtgct cggcacgttg cggcgccagc agctgccagc gtgcgcgcgt
     7801 acgctcgctg acgatgtcgt accagtactc gccgcaggga ttgaactgga acaggtgcac
     7861 gtcggtgcgt gtcgcgagcg cggccagcgc ctcgacgaac accggcgcga gcgcgccgag
     7921 cccgaagccg atcacgcgct cgggcagcgc gcacggcagc ggcgcgcccg tgccgaggcg
     7981 cgcgaccagc ctctcgaggc gcgccgcacc gtgcgtgctg gcgggatgct ccgctccgat
     8041 ctgctgcacc agcgcgcgcc acagcgaggc ctgccactcg ttgcccgcga gcgggcagtc
     8101 gggcgcgggc aagcctgctt cccaggccag cagccagtgc gggcggtaca gcaggtactg
     8161 gtcgaacagg gtggcgaggc gccggcacag cgcatggagc cgcctctccg gggtcgcggc
     8221 ctccaggtag cggcgcacgg gagcgaaggc gggctcctcg agcaggccgg gcagcacgcg
     8281 catcagccgg atcgcgagct gctccttgct ccaggcggtg gcgcccgccg gccccgcatc
     8341 gtccagcacc gcgcccgcca gcgcgttcag gaataccgcg ggcgagtcgt tgcgcaggtt
     8401 cgcggccacg cccaggcgcg ccgcgatgcc ctggcgcagc cacttggcca tgccgacgtt
     8461 cggcacgatc agccgctccg ccaccagcgg cggcgcaggc gccgcgccga gcagcgtgca
     8521 ggccgcgtcg cgcagcgcct ccatgtcgtt gccgtggtac tggtagagca agcgccgtcg
     8581 tcctggcccg cggggagcgg cgattgtgac ccagtgccac ggcaaccgga aggcggatac
     8641 gccgcgcgtc ggcgttgccg tgcgcgccga ccgtacgcgc attatcctgt ggcgcatgcc
     8701 accggccgga aacctctccc catgtccccg cacctgagca ccatcgtgct gctgctctgc
     8761 agcaacgttt tcatgacctt cgcctggtat gcgcacctga agaacctcgc cgacaagccg
     8821 tggatagtcg cggcgctgct cagctggggg gtggcgctgt tcgagtacct gttccaggtg
     8881 ccggccaacc ggatcgggca ccaggtgatg agcgtgggcc agctgaagat cctgcaggag
     8941 gtgatcacct tgctggtgtt cgtgccgttc tcgctgttct acctgcgcga gcgcctcacg
     9001 ctcgactacc tgtgggcggg cctgtgcatc ctgggtgcgg tgttcttcat gttccgcgcc
     9061 aaactcggcc tgtagcgttg cccgatccgt gaagcgccgc acgcgccgga catcgtgccc
     9121 gcacggggcg ctacgcagtg tgcccgggtg ctctacgctc tgatctgcgg cggcggttgt
     9181 tttgccaatg cgggaggacg gcggcagcag ggtgcaggcg ggatgtttgc gctagatgta
     9241 gcgccaatga tgaagttgta ccacaagata ttgtggaaac ttcgtggggc gcgcactact
     9301 atcgagcgaa cttgcacgtt tgcatagcct gtctatactg tatgcaaaca cagtatgtat
     9361 ggaagcacag ccatg
//
LOCUS       DAORUQ010000126         9305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_7992_length_9305_cov_6.395243, whole genome shotgun sequence.
ACCESSION   DAORUQ010000126 DAORUQ010000000
VERSION     DAORUQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7992_length_9305_cov_6.395243"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLW13_11160"
     CDS             <1..709
                     /locus_tag="PLW13_11160"
                     /inference="COORDINATES: protein motif:HMM:NF005853.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY70979.1"
                     /translation="GGGIGEGYANAFAARGMKVVVAELNEEGGERVAAGIRAAGGEAL
                     FVRTDVSDEASAKACAEAASSAYGGIDYLVNNAAIFGDMLKGGYLTVDLAYLEKFMRV
                     NAHGCLIMTRAALPYLQKRPGAAIVNQSSTAAWMNAGYYGVAKLTMNGITTSLARELG
                     PLGIRINAIAPGPTETSALQKTAGEYAQQMIKTMPLARLGQPQDMADAVLFLLSDQAS
                     WITGHILNVDGGQLMRV"
     gene            complement(883..3315)
                     /locus_tag="PLW13_11165"
     CDS             complement(883..3315)
                     /locus_tag="PLW13_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70980.1"
                     /translation="MKAPFRRSALCAAIFTTLAPAALAQIQLEEIIVTATKRTESLQD
                     VPISVNAVSGMKMEQVGITNLEKLTAYVPNFSMNQTGISSAITVRGISSGINQSFEQS
                     VGMYNDGIYFGKAQLTRLPLFDMERVEVLRGPQPILFGKNSIAGAVSLITAKPEDEFG
                     GSAQVLYEPNADEQDYRFVVTGPLTDTLSGRLSVLYREIDGWVENTALNDRDETQEEE
                     QVVRLGLHWQPTDALGIQFKYENAQFDTVGRNIELVGDVIREDLPPGTGTSYLTALPR
                     FVANLNSLIDAGVRTGPKITSFVPDDGEYNDKRTAGAWDTSENDVDNFLFDANYEWCD
                     HTLSFVTGYVEYDTSETYDVDYTAAPILDDSRLSEDYDQFSQEIRLTSPGGETVDYIV
                     GAFYQDSSLDFGDSINVPTDSLLRLLANPFAGISTRRVFEQDTEVWAAFGQATWNVTE
                     AWRLILGARYTQEDKDGYRKQAHFDTAGLEVAATDPRSALYNVLWGRLAIEPNELEDS
                     RSEDAFTPQVILQWDATGDLMLYASYVEGFKSGGYDNRSNAHPDPAVVVPGTGPNVVG
                     AWEFDKEEATSFEVGFKSTLADGAAELNGALFYTEYDDLQTSVFDGVVGFNVQNAGKA
                     TTQGMELDGRWLLHEYFTLSGSLGYLDFEFDDFDVAQCWFGQAQLEPDRVTNAALSQC
                     DASGERKEFTPEWKGVVTADFNYPVTDALELRAMVDLQYTDSYLWTPQLDPRTEQDAF
                     TKVNARLALAESSSDTWEVALIGSNLTDERTHSYGGTGVLAGTLTGGTGMAYYAFTDR
                     PRSYSLQGTYRF"
     gene            complement(3459..4520)
                     /locus_tag="PLW13_11170"
     CDS             complement(3459..4520)
                     /locus_tag="PLW13_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HQY70981.1"
                     /translation="MTATLFDPVQLGDIQLANRIVMAPMTRSRAGPGDAPTELNVEYY
                     RQRASAGLIVSEGVQPSANGKGYCRTPGLHTEPQVAGWKAVTDAVHAEGGRIVAQIMH
                     CGRVGSHLNKDPGAETVAPSAVRAKGQIYTDAAGMVDLDEPRALRLEEIPGVIGEYRH
                     ATQCALRAGFDGVELHCTSGYLPAQFLSTGTNQRTDAYGGSVQNRLRFVLEVLEAMTA
                     VAGSGRVGMRICPGNPFNDLHDDNPHETFAALLDAVSPMRLAYLHVIRSPRRSLDSFA
                     LAREHFRGPLIVNDSFDRASADRVLEAGEAAAVSFGRSYIGNPDLVRRLREGLPLADF
                     DLATLYTPGPKGYTDYPAA"
     gene            complement(4587..5216)
                     /locus_tag="PLW13_11175"
     CDS             complement(4587..5216)
                     /locus_tag="PLW13_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY70982.1"
                     /translation="MSASTDLLARIDRLESTEAIRQLAARYSLSLDMRDLDAHVNLFA
                     PDIRVSREKTGRAHLKRWLDDTLRLQFTGTSHHIGNHIIEFDDADHAHGVVYSKNEHE
                     TGPEWVIMQMLYWDNYERVEGTWYFRRRLPCYWYATDLNKPPIGDRKMRWPGREPYDG
                     AYHELFPSWKEFWANPPTGDEPEVAAPAPVGEFLKRMRRTTAEPRIRVR"
     gene            complement(5227..6024)
                     /locus_tag="PLW13_11180"
     CDS             complement(5227..6024)
                     /locus_tag="PLW13_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70983.1"
                     /translation="MAGRLDGKVALVTGGGTGIGAATARRFAEEGATVTVCGRRIEPL
                     EQVVHAIQADGGSARAVLLDVSDRQAFADVVTGIERQDGRLDILVNNAYSMVGATIAD
                     SSEEDWHANFRVTLDGTFHGIQAALRVMEFQRSGAIVNVSSVCGLLGSMYTAAYGAAK
                     AGVLSLTRTAALEGAPHNIRVNAVVPGVVMTPGTEAAIPTRAAQLATAAGVPLRRIAD
                     PLELANAILFLASDEASYITGIQLVVDGGKTCELATGAATMNEFDAT"
     gene            complement(6063..6890)
                     /locus_tag="PLW13_11185"
     CDS             complement(6063..6890)
                     /locus_tag="PLW13_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY70984.1"
                     /translation="MRLQGKVALVTGAGLGMGRAMAEVFAREGAAVVVVDLTARDAEE
                     TLALINARGDCCALTADVSDSAQVASVYAEVERRYGRLDVLVNNAGIGAAPNDGYDKF
                     YERMAAMNEQIARGEAPTVHLDHIIDMQDDGWQKVLDINLNGLFYNCREAVRLMIRCG
                     VKGSIINISSTAGLNGTGAVHYCSSKAAVLGFTKCLARELGGRGIRVNAICPGAMNTR
                     MMASISPDYAKAMLASIPLGRMGEPVEVANTALFLASDEASYFTGQTLAANGGWQML"
     gene            complement(7062..7709)
                     /locus_tag="PLW13_11190"
     CDS             complement(7062..7709)
                     /locus_tag="PLW13_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQY70985.1"
                     /translation="MTRRFEAFLFDFGGVFTDSPFTAVEDFGRAIGADPSRVSAIVFG
                     SYEHDGEHPWHRLERGEIALEAARESILALGREGGLEVDIYQLFARMAGNDAGAASRG
                     VLLEAVRGLRGAGYRTGIVTNNVREFGDGWRALIPVEELFEFVVDSSHAGVRKPDPRI
                     FALALERLGRPDPARCVFLDDHPANVAAAQALGMRAIHVGPDPAVTVEAIERLLD"
     gene            complement(7726..8853)
                     /locus_tag="PLW13_11195"
     CDS             complement(7726..8853)
                     /locus_tag="PLW13_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70986.1"
                     /translation="MSKWQAAQSLSRMDDYPLHQIADTMRHVGTSDRNFYDRYYFNLH
                     NSSGELFMVMGMGQYPTLAVQDAFVALRRGNTHRVVRASRELDDRADTSVGPIRIEVL
                     EGLQKLRFVLEPNEWGLAMDLVWEGAIPAFLEPRQYVRRKGRVFFDSVRFAQTGCWSG
                     WLEIDGERHAVTPDRWKGSRDRSWGIRPVGEHEPAGIHHGTHSMQGMWNYCPMQFDDH
                     SILYICNELDDGRRELEEAMRIWADPEREPEWLGRPEYEHTFDAAKEFITASTIRFPG
                     APGGAFEVQCTPLLPMYLFIGTGYGVDADWKHGMHKGPLVVEGLTLDHERDRGRFFGL
                     IDSPCRFETGGKVGWGLFEYCFMGPFERYGISNPLFWNLKL"
     gene            complement(8850..>9305)
                     /locus_tag="PLW13_11200"
     CDS             complement(8850..>9305)
                     /locus_tag="PLW13_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70987.1"
                     /translation="IDIDSLRYYAHWTTPPIMPKRYATWFFVARAPADDAVTVDGSEI
                     HEHQWVRPAEALEKRARGEIEMMPPTFVTLTELSACADVAEVLARVARRDPPVFEPHF
                     VVRAGLPTESLYAGDAGYAAADPDVPGSRHRVVMGHQAWQYHNEGGVAW"
BASE COUNT         1452 a         3171 c         3134 g         1548 t
ORIGIN      
        1 cggcggcggc atcggcgagg gctatgccaa tgccttcgcg gcacgcggca tgaaagtggt
       61 ggtggcggag ctgaacgagg aaggtggcga acgcgtcgcg gcgggcatcc gcgccgccgg
      121 cggcgaggcg ctgttcgtgc gcacggacgt gagcgacgag gcctcggcga aggcctgcgc
      181 cgaggcggcg tccagcgcct acggcggcat cgactacctg gtgaacaatg ccgcgatctt
      241 cggcgacatg ctcaagggcg gctatctcac cgtcgatctc gcctacctcg agaagttcat
      301 gcgcgtgaat gctcacggct gcctgatcat gacgcgcgcc gcgctgccct acctgcagaa
      361 acgcccgggt gcggccatcg tgaaccagtc ctcgaccgcg gcgtggatga acgcgggcta
      421 ctacggcgtg gcgaagctca cgatgaacgg catcaccacc tcgctggcgc gcgagctcgg
      481 cccgctcggc atccgcatca acgcgatcgc gcccggcccc accgagacca gcgcgctgca
      541 gaagaccgcc ggcgaatatg cccagcagat gatcaagacc atgccgctgg cgcgcctcgg
      601 ccagccgcag gacatggccg atgccgtgct cttcctgctc tcggaccagg cgagctggat
      661 caccggccac atcctcaacg tcgacggcgg tcagctgatg cgcgtgtagg tcagcattta
      721 tgccgacatg tgcttgcggg tgggcattcg tgccgacctg caaaagcctc tgggcgattt
      781 cgcgtggcgg gttcggcgtg gccgaaccct tgtcgggctg aagcccgacc tacatgcccg
      841 acccacgccc gacctgggag ccccgcccaa acgaaccggc gctcagaagc ggtaggtgcc
      901 ctgcaacgag tagctgcgcg gccggtcggt gaaggcgtag tacgccatcc ccgtaccgcc
      961 ggtcagcgtg ccggccagca cacccgtgcc gccatagctg tgcgtgcgct cgtcggtgag
     1021 gttgctgccg atcagcgcaa cctcccaggt gtcgctgctg ctctcggcca gcgcgaggcg
     1081 cgcattgacc ttggtgaatg catcctgctc ggtgcgcgga tcgagttgcg gcgtccacag
     1141 gtacgagtcg gtgtactgca ggtccaccat cgcgcgcagc tccagcgcgt ccgtcaccgg
     1201 gtagttgaaa tcggccgtca ccacgccttt ccattcgggc gtgaactcct tgcgctcgcc
     1261 gctggcatcg cactgcgaca gcgctgcatt ggtcacgcga tcgggctcca gctgcgcctg
     1321 cccgaaccag cactgcgcca cgtcgaagtc atcgaactcg aaatccaggt aaccgagcga
     1381 gcccgacagc gtgaagtact cgtgcagcag ccagcgtccg tcgagctcca tgccctgcgt
     1441 ggtggccttg ccggcattct gcacgttgaa gcccaccacg ccgtcgaaca ccgaggtctg
     1501 caggtcgtcg tactcggtat agaacagcgc gccgttcagc tccgcggcgc cgtcggccag
     1561 cgtggacttg aagccgacct cgaagctcgt cgcctcttcc ttgtcgaact cccaggcgcc
     1621 gaccacgttc gggccggttc caggtaccac cacggccggg tcggggtggg cattggaacg
     1681 gttgtcgtag ccgcccgact tgaagccctc gacatagctc gcgtagagca tcaggtcccc
     1741 ggtggcatcc cactgcagga tcacctgcgg cgtgaaggca tcctcgctgc ggctgtcctc
     1801 gagttcgttg ggctcgatgg cgagccgccc ccacagcacg ttgtacagcg ccgagcgcgg
     1861 atccgtggcc gcgacctcca ggccggcggt gtcgaagtgc gcctgtttgc ggtagccgtc
     1921 cttgtcctcc tgcgtatagc gcgcgcccag gatcaggcgc cacgcctcgg tgacgttcca
     1981 ggtcgcctga ccgaaggctg cccacacctc cgtgtcctgc tcgaacacgc gccgggtgct
     2041 gatgccggcg aacggattcg ccagcaggcg cagcaggctg tcggtcggca cgttgatact
     2101 gtcgccgaaa tcgaggctgc tgtcctggta gaaggcgccc acgatatagt ccacggtttc
     2161 gccgccgggc gaagtcagcc ggatttcctg gctgaactgg tcgtagtcct ccgacagccg
     2221 gctgtcgtcg agtatcggcg cggcggtgta gtcgacgtca taggtctcgc tggtgtcgta
     2281 ctcgacgtag ccggtcacga aactcagcgt gtggtcgcac cattcatagt tcgcgtcgaa
     2341 caggaaattg tcgacgtcgt tctcgctggt atcccaggcg cctgccgtgc gcttgtcgtt
     2401 gtactcgccg tcgtcgggca cgaagctcgt gatcttcggc ccggtgcgca caccggcatc
     2461 gatcagcgaa ttgaggttgg cgacgaagcg cggcagcgcc gtcaggtacg aggtgccggt
     2521 gcccggggga aggtcctcgc ggatcacgtc acccaccagc tcgatgttgc ggcccacggt
     2581 gtcgaactgt gcgttctcgt acttgaactg gatgcccagc gcgtcggtgg gttgccagtg
     2641 caggccgagg cgcacgacct gctcctcttc ctgcgtctcg tcgcggtcgt tcagcgcggt
     2701 gttctccacc caaccgtcga tctcgcggta cagcaccgac aggcgaccgc tcaacgtgtc
     2761 ggtgagcggg ccggtcacca cgaagcggta gtcctgctcg tcggcattag gctcgtagag
     2821 cacctgcgcc gaaccgccga actcgtcctc gggcttcgcg gtgatcaggc tgacggcgcc
     2881 ggcaatgctg ttcttgccga acaggatcgg ctgcgggccg cgcagcacct cgacgcgctc
     2941 catgtcgaac agcggcaggc gcgtcagctg cgccttgccg aaatagatgc cgtcgttgta
     3001 catgccgacg gactgctcga aggactggtt gatgccggag ctgatgccgc gcaccgtgat
     3061 cgccgaggaa atcccggtct ggttcatcga gaagttcggc acgtaggccg tcagcttctc
     3121 gaggttggtg atgccgacct gttccatctt catgcccgac accgcgttca cggagatcgg
     3181 cacgtcctgc aggctctcgg tgcgcttggt ggcggtgacg atgatctctt cgagctggat
     3241 ctgagccagg gccgcgggtg ccagggtcgt gaaaatagca gcacagagtg ccgagcgccg
     3301 gaacggtgct ttcatagcgt gtctcccaca ggttcttgta attggttctt gcagctacag
     3361 acaatgctgc agcgagcata gaccgccctg cgcgggaagg gcaaatgaaa tttcgcgggg
     3421 cacgggggca acgccaacgc cgcccccgcg cggtgcactc aggcggcggg atagtccgtg
     3481 tagcccttcg ggccaggggt gtagagcgtc gcgaggtcga agtccgcgag cggcagcccc
     3541 tcgcgcaggc gccgcaccag gtcggggttg ccgatgtagg agcggccaaa ggacaccgcc
     3601 gcggcttcac ccgcctcgag cacacgatcg gccgaggcgc ggtcgaagct gtcgttgacg
     3661 atcagcggcc cgcggaaatg ctcgcgcgcg agcgcgaagg agtccaggct cctgcgcggc
     3721 gagcggatca cgtgcaggta ggcgagccgc atcgggctca ccgcatcgag cagcgccgcg
     3781 aaggtctcgt gcgggttgtc gtcgtgcagg tcgttgaacg ggttgcccgg gcagatgcgc
     3841 atgccgacac gcccgctgcc ggccaccgcc gtcatcgcct ccagcacctc gagcacgaag
     3901 cgcaggcggt tttgcacgct gccgccgtag gcatcggtgc gctggttggt gccggtcgag
     3961 aggaattgcg ccgggaggta gccgctggtg cagtgcagtt ccacgccgtc gaagccggcc
     4021 ctcagcgcgc attgcgtggc gtgacggtat tcgccgatca cgcccgggat ctcctcgagg
     4081 cgcagcgcgc gcggttcgtc gaggtcgacc atgccggcgg catcggtgta gatctggccc
     4141 ttggcgcgca ccgccgacgg ggccacggtc tcggcgccgg gatccttgtt caggtggctg
     4201 cccacgcgcc cgcaatgcat gatctgcgcg acgatgcgcc cgccctcggc atgcaccgcg
     4261 tcggtgacgg ccttccagcc cgccacctgc ggctcggtat ggaggcccgg cgtgcggcag
     4321 taacccttgc cattggcgct gggctgcacg ccctcgctga cgatcagccc ggcactggca
     4381 cgctggcggt agtactccac attcagttca gtgggcgcgt cgcccgggcc ggcgcggctg
     4441 cgcgtcatcg gcgccatcac gatgcggttg gcgagctgga tgtcaccgag ttgcacggga
     4501 tcgaacagcg ttgcggtcat ggaaacagga ctcctgtgat ctggtcgcga atagggcgag
     4561 gccgccacag ggacggccgc gggatatcaa cgtacgcgga tgcgcggctc cgccgtggtg
     4621 cggcgcatgc gcttcagaaa ctcgccgacc ggcgcggggg cggcgacttc gggttcgtcg
     4681 ccggtcggcg ggttggccca gaactccttc cacgagggaa acagttcgtg gtacgcgccg
     4741 tcgtagggct cgcggccggg ccagcgcatc ttcctgtcgc cgatcggcgg cttgttgaga
     4801 tcggtggcgt accagtagca cggcaggcgg cggcggaaat accacgtgcc ctcgacacgc
     4861 tcgtagttgt cccagtagag catctgcatg atgacccact cgggcccggt ctcgtgctcg
     4921 ttcttggaat acaccacgcc gtgcgcgtga tcggcgtcgt cgaactcgat gatgtggttg
     4981 ccgatatggt gcgaggtgcc ggtgaactgc aggcgcaggg tatcgtcgag ccagcgcttg
     5041 aggtgcgcgc gcccggtctt ctcgcggctg acgcggatgt cgggcgcgaa caggttcacg
     5101 tgcgcatcga gatcgcgcat gtcgagcgac agcgagtagc gcgccgcgag ctggcgaatg
     5161 gcctcggtgg actcgagacg gtcgatgcgg gccaggaggt cggtggatgc gctcatgcag
     5221 cctccctcag gtcgcgtcga attcgttcat cgtggccgcc ccggtcgcca gctcgcaggt
     5281 cttgccgcca tcgaccacca gctggatgcc cgtgatgtag gaggcctcgt cggaggcgag
     5341 gaacaggatc gcgttggcca gttcgagcgg atcggcgatg cgccgcagcg gcacgcccgc
     5401 ggcggtcgcc agttgcgccg cgcgggtcgg gatcgccgct tccgtgcccg gcgtcatcac
     5461 cacgcccggc accacggcat tgacccggat attgtgcggc gcgccctcga gcgcggcggt
     5521 gcgcgtcagg ctcagcacac cggccttcgc cgcgccgtag gcggcggtat acatcgagcc
     5581 gagcagtccg cacaccgagg agacgttcac gatggcgccg ctgcgctgga attccatcac
     5641 gcgcaacgcg gcctggatgc cgtggaaggt gccatcgagc gtgacgcgga agttcgcgtg
     5701 ccagtcttcc tcgctcgagt cggcgatcgt ggcgccgacc atcgagtagg cgttgttgac
     5761 gaggatgtcg agccggccat cctggcgctc gatgccggtc acgacatcgg cgaaggcctg
     5821 gcggtcgctc acgtcgagca gcaccgcgcg cgccgaaccg ccatcggcct ggatcgcgtg
     5881 caccacctgc tccagcggct cgatgcgccg cccgcacacg gtcacggtcg cgccctcctc
     5941 ggcgaaacgc cgtgcggtgg ccgcacctat gcccgtaccg ccgccggtga cgagggcaac
     6001 cttgccgtcg agtcttcctg ccatgttcgt gtcctcgtga aaggtgggtg cgcgcgtggc
     6061 gctcagagca tctgccagcc gccattggcg gccagcgtct gcccggtgaa gtacgatgcc
     6121 tcgtcgctgg ccaggaacag cgccgtgttg gccacttcca ccggctcgcc catgcgcccg
     6181 agcgggatcg aggcgagcat cgccttggcg tagtcggggc tgatgctggc catcatgcgg
     6241 gtgttcatgg caccgggaca gatcgcgttg acgcggatgc cgcggccgcc gagttcgcgc
     6301 gcgaggcact tggtgaagcc gagcaccgcg gccttcgacg agcagtagtg caccgcgccc
     6361 gtgccgttca ggcccgcggt gctggagatg ttgatgatcg agcccttgac cccgcacctg
     6421 atcatcaggc gcacggcctc acggcagttg tagaacaggc cattcaggtt gatgtcgagc
     6481 accttctgcc agccgtcgtc ctgcatgtcg atgatgtggt cgagatgcac cgtcggcgcc
     6541 tcgccgcgcg cgatctgctc gttcatcgcc gccatgcgct cgtagaactt gtcgtagccg
     6601 tcgttgggcg ccgcgccgat gccggcgttg ttgaccagca cgtcgagccg cccgtagcgg
     6661 cgctcgacct cggcgtagac cgacgcgacc tgcgcgctgt ccgagacgtc cgccgtgagg
     6721 gcgcagcagt cgccgcgcgc attgatcagg gccagcgtct cttcggcgtc ccgcgcggtg
     6781 aggtccacca ccaccaccgc cgcgccctcg cgcgcgaaca cctcggccat cgcgcgaccc
     6841 atgccgagac ccgctccggt caccaaagcc accttgccct gcagtcgcat cgcgcgcact
     6901 ccttcttgtt cgccgcccat cgttgacccg ccactatagc gacgcgcatg cggcggcgaa
     6961 agcacgcaac cgcgcccgga atgtgcaaaa gagttcgcgc cgggcggcgc gatccatgtg
     7021 gcaagagtgc gaaaggagac cggtctgcgg gccggcggcg ctcagtcgag cagccgttcg
     7081 atcgcctcga ccgtgaccgc ggggtcgggc ccgacgtgga tggcgcgcat gccgagcgcc
     7141 tgcgccgccg ccacgttggc cgggtggtcg tcgaggaaca cgcaacgcgc gggatcgggg
     7201 cgcccgagcc gctccagcgc cagggcgaag atgcgcgggt ccggcttgcg caccccggcg
     7261 tggctggaat cgaccacgaa ctcgaacaac tcctcgaccg ggatcagcgc gcgccagccg
     7321 tcgccgaact cgcgcacgtt gttggtgacg ataccggtgc ggtagccggc accgcgcagc
     7381 ccgcgcaccg cctcgagcag cacgccgcgc gatgccgcac ccgcgtcgtt gcccgccatg
     7441 cgcgcgaaca actggtagat gtcgacctcg agcccgcctt cgcgcccgag cgcgaggatg
     7501 ctctcgcgcg ccgcctcgag agcgatctcg ccgcgctcca ggcggtgcca aggatgctcg
     7561 ccgtcgtgct cgtaggaacc gaacacgatg gcgctgaccc ggctcgggtc ggcgccgatc
     7621 gcgcgcccga agtcctcgac ggccgtgaac ggcgagtcgg tgaacacgcc gccgaaatcg
     7681 aacaggaagg cctcgaagcg acgcgtcatg ccctgccccc ccgcctcaca gcttcaggtt
     7741 ccagaacagc gggttgctga taccgtagcg ctcgaacggc cccatgaagc agtattcgaa
     7801 caggccccag cccaccttgc cgccggtttc gaagcggcag ggcgaatcga tcaggccgaa
     7861 gaagcggccg cggtcgcgct cgtgatcgag cgtgagccct tccaccacca gcggaccctt
     7921 gtgcataccg tgcttccagt cggcatcgac cccgtagccg gtgccgatga acaggtacat
     7981 cggcagcagc ggcgtgcact gcacctcgaa cgctcccccg ggcgccccgg ggaaacggat
     8041 cgtggaggcc gtgatgaact ccttcgccgc gtcgaaggtg tgctcgtact ccgggcgccc
     8101 cagccactcc ggctcgcgct ccggatcggc ccagatgcgc atcgcctcct cgagctcgcg
     8161 gcgcccgtca tcgagctcgt tgcagatgta caggatggag tggtcgtcga actgcatcgg
     8221 gcagtagttc cacatgccct gcatgctgtg cgtgccgtgg tggatgcccg ccggctcgtg
     8281 ctcgcccacc ggccggatgc cccaggagcg gtcgcggctg cccttccagc gatccggggt
     8341 caccgcgtgg cgctcgccgt cgatctccag ccagccgctc cagcagccgg tctgggcgaa
     8401 gcgcaccgag tcgaagaaca cgcgcccctt gcggcgcacg tactgtcgcg gctccaggaa
     8461 ggccgggatc gcgccctccc acaccaggtc catcgccagc ccccactcgt tcggctcgag
     8521 cacgaaacgc agcttctgca gcccttccag cacctcgatg cgaatgggac cgaccgaagt
     8581 gtcggcacgg tcatcgagct cgcgcgaggc ccgcaccacg cggtgcgtgt tgccgcggcg
     8641 cagcgcgacg aaggcgtcct gcaccgcgag cgtggggtac tggcccatgc ccatcaccat
     8701 gaacagctcg ccactggagt tgtgcaggtt gaagtagtag cggtcgtaga agttgcggtc
     8761 gctggtgccg acgtggcgca tcgtgtcggc gatctggtgc agcgggtaat cgtccatccg
     8821 cgacaatgac tgcgccgcct gccatttgct caccatgcca cgcccccctc gttgtggtat
     8881 tgccatgctt ggtgccccat caccacgcgg tggcggctac cgggtacgtc gggatccgca
     8941 gcggcatagc cggcatcacc ggcgtagagc gactccgtcg gcagccccgc gcgcaccacg
     9001 aagtgcggct cgaacaccgg cggatcgcgt cgcgcgacgc gcgccagcac ttccgccacg
     9061 tccgcgcagg cgctcagctc ggtcagggtc acgaaggtcg gcggcatcat ctcgatctcg
     9121 ccgcgcgcgc gcttctcaag cgcctcggcc gggcgcaccc actggtgctc gtggatctcg
     9181 ctgccgtcca ccgtgaccgc atcatccgcg ggcgcgcgcg ccacgaagaa ccacgtggcg
     9241 tagcgcttgg gcatgatcgg cggcgtggtc cagtgcgcgt agtagcgcag cgaatcgatg
     9301 tcgat
//
LOCUS       DAORUQ010000127         9245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8068_length_9245_cov_7.021219, whole genome shotgun sequence.
ACCESSION   DAORUQ010000127 DAORUQ010000000
VERSION     DAORUQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_9245_cov_7.021219"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="efp"
                     /locus_tag="PLW13_11205"
     CDS             complement(<1..536)
                     /gene="efp"
                     /locus_tag="PLW13_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQY70988.1"
                     /translation="MASFSTNEFKPGLKVILDGDPCSILENEYVKPGKGQAFNRVKLR
                     NLKNGRVWERTFKSGDSLEGADAVDKELQYLYNDGEFWNFMDPETFEQYTASKDIVGD
                     AGQWLKEQDTCTVTLWNDSPLTVSPANFVELEIVETDPGLRGDTATGGTKPAKLSTGA
                     TVRVPLFLDVGEVVRVDTR"
     gene            803..>2724
                     /locus_tag="PLW13_11210"
     CDS             803..>2724
                     /locus_tag="PLW13_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY70989.1"
                     /translation="MFRDAGHSVRAHRITSLRDLDDMLRDEQWDLFIAHEKHPEVAPA
                     EALALLTGRASDLPCIVRAAEPLGAEALGWLRAGARDVVGADDGARLLLAACREIRAR
                     RAHRELATLRVQHAEIAERCELLLAASRDAIAYVVDGMHVHANELYAELFGYAGVEEL
                     GSIPLVDLIAAQKQQEFKDALKRYRAHPEAQTAIDFTGTRADGSEFAGQLVLTSASFE
                     GEPCMQVLVRAAPAGTAAAPAPATAGAGGLAVLASALSANAGGQLLLIAVDGYNQHCR
                     HLGVGAANRLVDELGAFIARSVGWTQAPLRAADGVLAVLLADSDPERALAQARRAVEA
                     VAGHIHEVGTQSVSCSICVEVCPLGEGRDATVDALLDRCWSALLGTVERAQRLRAADP
                     ERIGLASAAAPDAGAVAATASGPSIEEALRRGELRILFQPIVSLRGDSSEYYEAQARH
                     QPSDRPALDWLAETNPGASSAELDQLVAQQALQQLAAHRAAHPETRLILPIGGGSMLD
                     ADFADWLAATLRAAAVPPDCLVIAIAHRTASSHLKQAKQLAERLSSLGCQLCVAEVHS
                     GDNPLPELLHLRPQFARIAEALAPALADTESTSTLLKPLVESLHREQIASIMPGVEGA
                     GVLAVLWQLGVNFIQG"
     assembly_gap    2725..2824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2825..4474)
                     /locus_tag="PLW13_11215"
     CDS             complement(2825..4474)
                     /locus_tag="PLW13_11215"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY70990.1"
                     /translation="MRPLSSLFVLLLAVCVGCVEAAPGARPNFLVIVADDLGYSDLGA
                     FGGEISTPSLDALASDGLRLSGFHTAATCSPTRAMLLTGTDHHLAGMANMSELITPSQ
                     RGRPGYDGHLADDVVTVAELLRDAGYRTLMSGKWHLGTRPEDDPSRRGFERVFSLIEG
                     GHNHFGKPNLPPPELGGAHYTEDGRPASIPADFYSSDYFTDKLLQFMTEDRGRPFFAY
                     LPFTAPHWPLHAPPEIIARYAGRYDAGWEILLRERLARQRALGLLPPGASEELPPTLR
                     DWESLSPEEQRRQARKMEIYAAMVERMDWNVGRVIESLRASGRLEDTVIVFFSDNGAA
                     PDTVAGLLKKVEGFAPLEEGAFADWGSAESMLSYGPHWAQAATAPRRLYKSVVSEGGI
                     ASPAIVRFPGFARQNGNLGASFATVMDVAPTLLEIAGVAPPGTSYRGRAVEPMRGRSM
                     LPYLQGRAERVHAADEAFGWELFGQRALRQGDWKAVWLSLPNGPGRWALFDLARDPGE
                     RHDLAQQEPARLEALVRLWDGYAKDMGVILEEQVVSPHTNL"
     gene            complement(4487..6661)
                     /locus_tag="PLW13_11220"
     CDS             complement(4487..6661)
                     /locus_tag="PLW13_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY70991.1"
                     /translation="MKPTLKPLARSALIAGASLATLATVPLGHAQSAGLEEVIVTAQK
                     REENLQDTPISLVALDARQLEKLGISSIGDLGSNVPNLQLSPHPNSATTPRVFIRGVG
                     NFDDQITQDPSVAVYMDGVYVGRNQGMGVEVADIERIEVLRGPQGALYGRNATGGAIN
                     FLTRAPELGEWGFSQQLTAGNRDRFRSRTMLNAPLGEQLAARLFYLNSRQDGFVRNAG
                     RGTESYGAEDHDAVRAELLFRPSEALDVRYAFDRSTMEDSPYYLAPVTRGVPPQRPSR
                     SHAGNQLIKRGDVTTLGHHLTLNWLLHEDLTLRSITAYRDLDSYIFQDYLSGTGRPNT
                     PLFIDNYVDQDQFSQEIQLLGSAFGEQLEYTLGLYYFTEDGTGDTVNWLPGFGIRQFT
                     GAEIENEALAAYGQATYTPVALERLHITVGLRSSKDEREADLTRYNVLLSTNTILPAS
                     VGRGSGDKDFDDLSPSLTVAWDLSEDASFYAKVADGYKTGGFNMRASTIAYFEQGFDE
                     ETLRSYEVGLKAEWMDNRLRSNIAVFEADYQDIQINAQTSLTNPAVADILNGGEATIQ
                     GAELELSALVTDSLTLTLQYGYLDAGYDEVLDGFGRDVTDQFVFVNAPEHSYTVDVSW
                     DIAATPVGELAANLNYTWQDEKYITATTNFGVYTIDAYGLLNARVTLSEIPVAGQGQL
                     TLAAWGKNLEDNEYYFLNAPGFGGYVAWGEPRSYGIDLSYEF"
     gene            6882..8078
                     /locus_tag="PLW13_11225"
     CDS             6882..8078
                     /locus_tag="PLW13_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY70992.1"
                     /translation="MSLLNEALLIGAAQGALLCIVILSLPSANRTANRLLASYVGLES
                     LHLFFLYTSYMDDAAAPDAWLRLLFGLRALDGPAVYLYVRALSEADFRWHPGLLRHLW
                     VLLFLFGWFAVLISDPAWLALSTRELQQLPSTVAMSAYQSLVLAAYGLVAWRRLAAHQ
                     RRLRQALSAVDRVNLDWLSWLMGALIATNALHLALDLLRLLGLVGADSKVLVNLCMTL
                     LLIYLISIGGLRQPKVFTDPVREALAAVDEPAPAVETGQNGGADRSKYQKSGLDEARR
                     TEIWNRLQALLQQERPHLDPGLSLPGLARLLGVRPQELSEVINTAYGGSFYDLVNRAR
                     VDMAKVLLREPRARRRKMLDIALSVGFSSQSTFYSQFRKQTGMTPTVWRDKGEGWPEG
                     GETVDA"
     gene            complement(8113..9222)
                     /locus_tag="PLW13_11230"
     CDS             complement(8113..9222)
                     /locus_tag="PLW13_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007997428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQY70993.1"
                     /translation="MLPAARAATPDPATPWHAFCDRLKEAGDQILRPEAPQDPFNRAE
                     GYRYLSRLLRAALEQEVECSDPQFPVLYQLSNETIKIGADNPDNFYQNAQIDGRYDYR
                     IRGSRGTVNYMVLSTKAGSYGKSSSLQHTGAIDTTTLQVAPDGSFEILLSAARKPGNW
                     LPMSAESSMLLVRQTFLDRGTERPAALEIECLNGPARPAPLDPERLSVQLLAAADFVQ
                     GTARIFADWSQRFKANPNAFTVLDQAEFLRQGGDPAIFYAHGYWALGPEQALLIDVPP
                     SENEFWNLQIDNYWMESLDYRYFTIHLNKRTTRYRPDGSAVLVLAHRDPGVPNWLDTA
                     GHDLGTMLYRVVRGADPAYPRTRVVALAEVREALG"
BASE COUNT         1403 a         3077 c         3142 g         1523 t
ORIGIN      
        1 cgggtgtcca cgcgaaccac ttcgcccacg tcgaggaaca gcggcacgcg cacggtggcg
       61 ccggtggaca gcttggcagg cttggtgccg ccggtggcgg tatcgccgcg cagccccggg
      121 tcggtctcga cgatctccag ctcgacgaag ttcgccggcg acacggtgag cggcgagtcg
      181 ttccacagcg tcaccgtgca ggtgtcctgc tccttcagcc actggccggc atcgcccacg
      241 atgtccttcg aggcggtgta ctgctcgaag gtctccgggt ccatgaagtt ccagaactcg
      301 ccgtcgttgt agaggtactg cagctccttg tcgaccgcgt cggcgccttc caggctgtcg
      361 ccggacttga aggtacgctc ccacacgcgg ccgttcttca ggttgcgcaa cttcacgcgg
      421 ttgaaggcct gccccttgcc gggcttgacg tactcgttct ccaggatcga gcagggatcc
      481 ccgtccagga tcaccttcag gccgggcttg aattcattgg tggaaaagga cgccatgtgt
      541 gcctctgcag ggttcgcggc aaaatgatgg gcgcggatga tactccgatc ggccgctcga
      601 tggcagcgtg gcatgggtca cagctcgcgc ggcccgtccc ctggcccggt tggcgccctc
      661 gccgggggtc tcctacactt gccggaccgg ccctgcgacg atggcgtcgc ggccctgatg
      721 gcccattcga atgccgcata acgacaatct acagctcctg gtcagctgcg aatcacaggc
      781 tgcgctggaa agctgcctcg cgatgtttcg tgacgccggt cacagtgtgc gcgcgcaccg
      841 catcacctcg ctgcgcgatc tcgacgacat gctgcgtgac gagcagtggg acctgttcat
      901 cgcgcacgag aaacaccccg aagtcgcgcc cgccgaggcg ctggccctgc tcaccggacg
      961 cgcgagcgat ctgccctgca tcgtgcgcgc cgccgaaccg ctcggggccg aggcgctggg
     1021 ctggctgcgc gccggtgccc gcgacgtggt cggcgccgac gacggcgcgc ggttgctgct
     1081 ggcggcctgc cgcgagatcc gcgcgcgccg cgcgcaccgc gagctcgcga cgctgcgcgt
     1141 gcagcacgcc gagatcgccg agcgctgcga gctgctgctc gcggcctcgc gcgacgccat
     1201 cgcctatgtc gtcgatggca tgcacgtgca tgccaacgag ctctacgccg aactgttcgg
     1261 ctacgcgggt gtcgaggagc tgggctccat tccgctggtc gacctgatcg cggcgcaaaa
     1321 gcagcaggag ttcaaggacg cgctgaagcg ttaccgcgcc catcccgagg cgcagacggc
     1381 gatcgacttc accggcacgc gcgccgacgg cagcgagttc gccgggcagc tcgtgctcac
     1441 gagcgcgagc ttcgagggcg agccctgcat gcaggtgctg gtgcgcgccg cgcccgccgg
     1501 aactgccgcg gcacccgcgc cggcaacggc gggcgccggt ggcctggccg tgctggcttc
     1561 ggccctttcc gccaacgcgg gcgggcagtt gctgctgatc gcggtggacg gctacaacca
     1621 gcactgccgc catctcggcg tcggcgcggc gaaccggctg gtggacgaac tgggcgcctt
     1681 catcgcgcgc agcgtcggct ggacccaggc accgctgcgc gccgccgacg gcgtgctcgc
     1741 cgtgctcctg gcggacagcg acccggagcg tgcgctcgcg caggcgcgcc gcgccgtcga
     1801 ggcggtcgcg gggcacatcc acgaagtcgg cacgcaaagc gtgagctgca gcatctgcgt
     1861 cgaggtctgt ccgctcggcg aggggcgcga cgccacggtg gacgccctgc tcgaccgctg
     1921 ctggagcgcg ctgctcggca ccgtcgagcg cgcgcagcgc ctgcgcgccg ccgaccccga
     1981 gcgcatcggg ctcgccagtg ccgcggcgcc ggacgcaggc gcggtcgcgg cgacggccag
     2041 cggcccctcg atcgaggagg cgctgcgccg cggtgagctg cgcatcctgt tccagccgat
     2101 cgtcagcctg cgcggcgaca gcagcgagta ctacgaggcg caggcgcggc accagccttc
     2161 cgaccgccct gcgctcgact ggctggccga gaccaacccg ggcgcatcga gcgccgagct
     2221 cgaccagctg gtggcgcagc aggcgctgca acagctggcc gcgcatcgcg ccgcgcatcc
     2281 cgaaacgcgc ctgatcctgc cgatcggcgg cggcagcatg ctggatgccg atttcgcaga
     2341 ctggctggca gcgacactgc gcgcggccgc ggtgccgccc gattgcctcg tgatcgcgat
     2401 cgcgcaccgc accgcgagct cgcacctgaa gcaggcaaag caactcgccg agcggctgtc
     2461 cagtctcggc tgccagctgt gcgttgccga ggtgcacagc ggcgacaacc cgctcccgga
     2521 actgctgcac ctgcgtcctc agttcgcgcg catcgccgag gcgctggcgc cggcgctggc
     2581 cgacaccgag agcaccagca cgctgctcaa gccgctggtc gaatcgctgc accgcgagca
     2641 gatcgcctcg atcatgccgg gagtggaagg cgcgggcgtg ctcgccgtgc tgtggcagct
     2701 cggcgtcaat ttcatccagg gcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnctagag gttggtgtgc gggctgacga cctgctcctc caggatcacg cccatgtcct
     2881 tcgcgtagcc gtcccatagg cgtaccagcg cttccagccg agccggctcc tgctgcgcga
     2941 gatcatgccg ttcgccggga tcgcgcgcca agtcaaagag cgcccagcga ccgggtccgt
     3001 tgggcaggct cagccagacg gccttccagt cgccttggcg cagggcgcgc tgcccgaaga
     3061 gttcccagcc gaaggcctcg tccgctgcgt gcacgcgctc cgccctgcct tgcaggtagg
     3121 gcagcatcga gcgcccgcgc atcggttcca ccgcgcgccc gcggtagctc gtccccgggg
     3181 gcgcgacgcc ggcaatctcg aggagcgtgg gcgccacgtc catcacggtg gcgaaggacg
     3241 cgcccaagtt gccgttctgc cgcgcaaagc ccggaaagcg gacaatcgcc gggctggcaa
     3301 tgccgccttc gctcacgacc gacttgtaga gccggcgtgg cgccgtcgcc gcctgcgccc
     3361 agtgtggccc gtaggacagc atcgattcgg cgctgcccca gtccgcgaac gcgccctcct
     3421 cgagcggcgc aaagccctcc accttcttca agaggccggc cacggtatcc ggcgcggcgc
     3481 cgttgtcgga aaagaacacg atcacggtgt cctcgagtcg cccgctcgcg cgcagcgact
     3541 cgatcacgcg gcccacgttc cagtccatcc gctcgaccat ggctgcgtag atctccatct
     3601 tccgcgcctg gcgacgctgc tcctccgggg agagggattc ccagtcgcgc aacgtcggcg
     3661 gcagctcctc gctggcgccg ggcggtagca gcccgagcgc gcgctggcgg gccagccgtt
     3721 cgcgcagcag gatctcccag ccggcgtcgt agcgtcccgc gtagcgagcg atgatctcgg
     3781 gcggcgcatg cagtggccag tgcggggccg tgaacggcag ataagcgaag aagggcctgc
     3841 cgcggtcctc cgtcatgaac tgcagcaact tgtcggtgaa gtagtcggaa gagtagaagt
     3901 ccgcggggat ggaggccgga cgtccgtcct ccgtgtagtg ggcgccgccc agctcgggcg
     3961 gcggcaggtt cggcttgccg aagtggttgt gtccgccctc gatcagcgag aagacccgtt
     4021 cgaatccgcg gcgcgaggga tcgtcctcgg ggcgtgtgcc cagatgccac ttgccgctca
     4081 tcagcgtgcg gtagccggca tcgcgcagga gctccgcaac ggtcaccacg tcatctgcga
     4141 ggtgaccatc gtagccgggt cgcccgcgct gtgaaggggt gatcagctcg ctcatgttgg
     4201 ccatgccggc aaggtggtgg tcggtacccg tcagcagcat ggcgcgcgtg ggcgaacagg
     4261 tcgccgcagt gtggaagccg ctcaggcgca gcccgtcgct cgccagggca tcgaggctcg
     4321 gtgtgctgat ctcgccaccg aatgcaccca ggtccgaata gccgaggtcg tcggcgacga
     4381 tgacgagaaa attcggtcgc gcgccgggcg cggcctccac acacccgacg caaaccgcca
     4441 gcagcagaac gaaaaggctg gacagaggac gcatggccgg cacggctcag aactcgtagc
     4501 tcaggtcgat gccgtagcta cgcggctcgc cccacgcgac gtagccgccg aagccgggcg
     4561 cgttgaggaa gtagtactcg ttgtcctcca ggttcttgcc ccaggccgcg agggtcagct
     4621 gtccctgacc ggcgacggga atctccgaaa gcgtgacccg ggcattcagc agcccgtacg
     4681 cgtcgatggt gtagaccccg aagttggtgg tggcggtgat gtacttctcg tcctgccagg
     4741 tgtaattgag gttcgcggcg agctccccga ctggcgtcgc ggcaatgtcc cagctgacat
     4801 ctaccgtgta gctgtgctcg ggcgcgttga cgaacacgaa ctggtcggtc acgtcgcgtc
     4861 cgaatccgtc cagcacctcg tcgtagccgg catcgagata gccgtactgc agggtcagcg
     4921 tcaggctgtc ggtaaccagt gcgctcagtt ccagctcggc cccctggatc gtcgcctcgc
     4981 caccgttgag gatgtccgcc actgcggggt tggtcaggct cgtttgggcg ttgatctgga
     5041 tgtcctggta gtcggcctcg aagaccgcga tgttgctgcg caggcggttg tccatccact
     5101 cggccttcag gcctacttcg taggagcgca gcgtctcctc gtcgaagccc tgctcgaaat
     5161 aggcgatcgt ggaagcacgc atgttgaagc cgccggtctt gtagccgtcg gcgaccttgg
     5221 cgtagaaact ggcgtcttcg ctcagatccc aggccaccgt caagctgggg ctgaggtcgt
     5281 cgaagtcctt gtcgcccgat ccgcgcccga cagaggccgg caggatcgtg ttcgtggaga
     5341 gcaacacgtt gtagcgggtg aggtccgcct cgcgctcgtc tttggaagag cgcagtccca
     5401 ccgttatgtg cagcctttcg agcgcgaccg gcgtataggt cgcctggccg taggcggcca
     5461 gcgcctcgtt ttctatttcg gcgccggtga actggcggat gccgaaaccg ggcagccagt
     5521 tcacggtgtc acccgtgcca tcctcggtga agtagtaaag gccgagcgtg tactcgagtt
     5581 gctcgccgaa ggcgctgccg agcagctgga tctcctggct gaactggtcc tggtcgacgt
     5641 agttatcgat aaacaggggg gtattggggc gccccgtgcc ggagagatag tcctgaaaaa
     5701 tgtagctgtc gaggtcgcga taggcggtga tcgagcgcag cgtcaggtct tcgtgcagca
     5761 gccagttcag cgtcaggtgg tgaccgagag tggtgacgtc gccgcgcttg atcagctggt
     5821 tgcccgcgtg gctgcggctc gggcgctgcg gcggcacccc gcgcgttacc ggtgccaggt
     5881 agtacggcga gtcctccatg gtcgagcgat cgaaggcgta acgcacatcg agcgcctccg
     5941 acgggcggaa cagcagctcc gcgcgcacgg cgtcgtgatc ctccgcaccg tagctctcgg
     6001 tacctcgccc ggcattgcgc acgaagccat cctggcggga attcagatag aacaggcggg
     6061 cggcgagctg ttcgccgagc ggggcgttga gcatggtgcg cgaacggaag cggtcccggt
     6121 tcccggccgt cagctgctgg ctgaaccccc attcgcccag ttccggggca cgggtcagga
     6181 aattgatggc gccgccggtg gcgttgcgtc cgtacagcgc gccctgcggc ccacgcagga
     6241 cctcgatgcg ttcgatgtcg gccacctcga cgcccatgcc ctggttgcgg ccgacgtaga
     6301 ccccgtccat gtatacggcg acgctcggat cctgcgtgat ctgatcgtcg aagttcccga
     6361 ccccgcggat gaatacccgc ggcgtggtgg cgctgttggg gtgcgggctc aactgcaggt
     6421 tgggcacgtt gctgccgagg tctccgatgc tggagatgcc gagcttctcg agttggcgcg
     6481 cgtccagcgc gaccagcgag atcggcgtgt cttgcaggtt ctcctcgcgc ttctgggcgg
     6541 tcacgatcac ttcctccagt cctgcggact gggcatgccc gagcggcaca gtggcgagcg
     6601 tcgcgagcga ggcgccggcg atcagggccg atctggccaa aggcttcagc gtgggtttca
     6661 tgtcggtgtc tcctgccgtg ggtctggaac gcctgaagcg gcgtgttgtt atgcgacttc
     6721 tgcgatacga atatggcgca ggcacggggc gcccgcctcc ggctgcatcg ggagactctc
     6781 cgcgccgatc ggcgcggaaa ccgcccgcgg gggcttggag cgcgcggagc cagccgctaa
     6841 ggtgggaccc ggcggaactc aaggctcgga tggcggcgaa catgtcactc ctcaacgagg
     6901 cgttgcttat cggtgcggcc cagggggcgt tgctgtgcat cgtcatcctg agcctgccgt
     6961 cggccaaccg cacggccaat cgcctgctgg catcctacgt cgggctcgag tcgctgcacc
     7021 tcttcttcct ttacacgagc tacatggacg acgcggccgc gccggacgcg tggctgcgcc
     7081 tgctgttcgg tttgcgtgca ctcgacggac cggccgtcta cctgtacgtg cgcgcgctca
     7141 gcgaagcgga tttccgctgg caccccgggc tgctccggca cctgtgggtg ctgctcttcc
     7201 tgttcggctg gttcgcggta ctgatcagcg acccggcctg gctcgccctg tcgacccggg
     7261 aactgcagca gctgcccagc accgtcgcga tgtcggccta ccagagcctg gtgcttgcgg
     7321 cctacggcct ggtggcctgg cgcaggctgg cggcgcacca gcgccggctg cgtcaggcgc
     7381 tgtcggcggt cgaccgggtc aacctcgact ggctgtcgtg gctgatgggc gcgctcatcg
     7441 cgaccaatgc gttgcatctc gcgctcgacc tgctgcgact gctgggactg gtaggcgccg
     7501 attccaaggt actggtgaac ctgtgcatga cactgctgct catctacctg atttcgatcg
     7561 gcggactgcg ccagcccaag gtgttcaccg acccggtgcg cgaggcactc gcggcggtcg
     7621 acgaacctgc acccgcagtg gaaaccgggc agaacggcgg cgcggatcgc agcaagtacc
     7681 agaaatccgg cctcgacgag gctaggcgca cggagatctg gaaccgtctg caggcgctgc
     7741 tgcagcagga gcgaccgcac ctggacccgg gactgagcct gcccggcctc gctcgcctgc
     7801 tcggcgtgcg gccgcaggag ctcagcgagg tgatcaacac cgcctacggc ggcagtttct
     7861 atgatctggt caatcgcgcg cgcgtcgaca tggccaaggt cctgctgcgc gaaccgcgcg
     7921 cccgccgtcg caagatgctg gacatcgccc tgtcggtggg cttcagcagc cagtccacgt
     7981 tctacagcca gttccgcaag cagaccggca tgaccccgac ggtctggcgg gacaagggcg
     8041 agggctggcc ggagggcggc gagaccgtcg acgcctagac gccaaagtgc tgccggacgt
     8101 gggcgcagcg catcagccga gagcctcccg cacctccgcc agcgccacga cccgcgtccg
     8161 gggataggcc ggatccgcac cgcgtaccac gcgatagagc atggtgccca agtcgtggcc
     8221 cgcggtgtcg agccagttcg gcacgccggg atcgcggtgc gccagcacca gcacagcgct
     8281 gccgtcgggc cggtagcggg ttgtgcgctt gttcaggtgg atggtgaaat agcggtaatc
     8341 aagcgactcc atccagtagt tgtcgatctg caggttccag aattcgtttt cgctgggtgg
     8401 cacgtcgatc agaagcgcct gttcgggccc gagcgcccag tagccatgcg cgtagaagat
     8461 cgccggatca ccgccctggc gcaggaactc ggcctgatcg agcaccgtga acgcgttcgg
     8521 gttcgccttg aaacgctgag accagtcggc aaagatccgc gccgtaccct gtacgaagtc
     8581 ggcagccgcg agcaactgca cggacagccg ctcagggtcg agcggcgccg gccgcgccgg
     8641 tccgttcaga cactcgatct ccagcgcggc gggcctctcc gtgccgcggt cgaggaaagt
     8701 ctgccgtacc agcagcatcg agctctctgc gctcatcggc agccagttgc cgggcttgcg
     8761 cgcggcgctc agcaggatct cgaaactgcc atccggcgcg acctgcagcg tcgtggtgtc
     8821 gatggcgccg gtatgctgca gcgaactgct cttgccatag ctgccggcct tggtggacag
     8881 caccatgtaa ttcaccgtgc cacggctgcc gcggatgcga tagtcgtaac gaccgtcgat
     8941 ctgcgcgttc tggtagaagt tgtccgggtt gtcagcgccg atcttgatgg tttcgttgga
     9001 gagctggtaa agcaccggga actgcgggtc cgagcattcc acctcctgct ccagcgcggc
     9061 gcgcagcagg cgcgagagat agcggtaacc ctcggcacga ttgaacggat cctgcggcgc
     9121 ctccggccgc aggatctggt ccccggcttc cttcagtcga tcacagaatg cgtgccaagg
     9181 cgtggcggga tccggcgtgg cggcgcgcgc ggccggcagc atgctgccca ggccgagggc
     9241 gctga
//
LOCUS       DAORUQ010000128         9120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8225_length_9120_cov_5.717264, whole genome shotgun sequence.
ACCESSION   DAORUQ010000128 DAORUQ010000000
VERSION     DAORUQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_9120_cov_5.717264"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PLW13_11235"
     CDS             <1..1300
                     /locus_tag="PLW13_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY70994.1"
                     /translation="QHFFVNGAMGTNSFFGMVELNPFEERERGAQEVQSDLTTRLAGV
                     SGLRINAFNMPSIPGASPDLPIKFVVSGTGSYELLFQVAQDIERKARESGLFVYAESE
                     LRFDKPEVRLDIDRDKAAQLGIDVAAIGSTLSTFLNENESSRLSLDSRSYEVILQLER
                     EYRLSADMLDTYYLRTGSGELVPLSAVVSQARENRPNKLTEFQQLNSATVGALMAPGV
                     SLGQGIAWFEETGRSLLPAGFRADYVGQSRQFMTEGNVLLFTFLFSFVLIFLVLAAQF
                     ESFSDPLTVLVSVPLSVCGALVPLALGMATMNIYTQVGLLTLIGLISKHGILIVDFAN
                     RQQEIRDAGAVDAVIEAASVRLRPILMTTAAMVFGVMPLLFASGAGAQARVSIGIVIT
                     FGMLVGTLFTLFVVPVIYSYVARRHVRMVDDQVVRKRDLS"
     gene            1542..1793
                     /locus_tag="PLW13_11240"
     CDS             1542..1793
                     /locus_tag="PLW13_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70995.1"
                     /translation="MTPDIFKPLHNAIIWAQHCADPLIWREAVRRFDAADRALGGLPC
                     RERLHLEERLYGMLPSDWPLWMESCHQSMSAMQRRALTH"
     gene            1864..3312
                     /locus_tag="PLW13_11245"
     CDS             1864..3312
                     /locus_tag="PLW13_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQY70996.1"
                     /translation="MQESTGVQRLLRGARLCSGPLAAALWFALGASAMAHDARVVLGL
                     AIWMALWWMTEAVPLAATALLPLVILPLFTSIGFGAAAAPYASNIVFLFMGGFMLGLA
                     LQRCGLHRRIALAMLVTVGSGQRRLVGGFMLVTALLSMWLSNTATMMMLAPIGISVLQ
                     TRREQRPGAEADGSEAFGAALILGIAYAASIGGMGTPIGTPPNLIMSGYLRAHYGIDV
                     TMLGWMRVGVPVILLLLPLAWLWLCFGAFRLPAAGFAGDEAQLRAQRARLGAASAAER
                     RTGAVFLLVALGWVLRPQLVAWFGLPGLDDGVIALIGALLLFVLPAQGLEGERLLDWN
                     TARGIPWDILLLFGGGLSLAAALTASGADAPIAQWASGLGSVPVALIVGVIAVAIVFT
                     GELTSNTAAATAIMPVLAGLASARGIDPLPLFMVATLAGSCGFMLPVATPPNAIAYGT
                     GFVPLRAMLRAGFGLNLIAVAVVVGFVWFVAF"
     gene            3323..4447
                     /gene="mtnA"
                     /locus_tag="PLW13_11250"
     CDS             3323..4447
                     /gene="mtnA"
                     /locus_tag="PLW13_11250"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQY70997.1"
                     /translation="MTVLPGWERSPAFVFDAARCALSVLDQAHLPFEQRYLQPGSVAD
                     CARAIRDMNVRGAPLIGLVAAAGMAFAARADASDAALEAAAALLIASRPTAVNLRWAV
                     EDLQAVLRTAPLAARAAAAAARVLALQHSESACCARIGEHGLGIMRALADTPRVRERG
                     VLNVLTHCNAGWLATGAWGTALAPVYRAAAAGIAVHVWVDETRPRNQGARLTAWELGE
                     GGIAHSIVADNCAGHLMQRGLVDVCIVGSDRTTRAGDVCNKIGTYQKALAAADNGVPF
                     YVALPCSTIDWTITDGVREIEIEERDASEVLQIEGAGADGVLRSVRHAPLASRAANFA
                     FDVTPARLVTGLICEHGVFAADATALAPLRAVAGAAPERV"
     gene            4480..5481
                     /locus_tag="PLW13_11255"
     CDS             4480..5481
                     /locus_tag="PLW13_11255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQY70998.1"
                     /translation="MENSSGLADLIGNTPLVRLRQVSEQTGCEILGKAEFMNPGGSVK
                     DRTALGIIRDAERRGVLRQGGRIVEGTAGNTGIGLSLLANALGYRTTIVIPETQSPEK
                     IEMLMLCGAELRLVPAVPASDPNHYVKISGRLAQEYAARETQGAVWANQFDNTANRAI
                     HRDTTGREIWEQTRGRVDGFICSVGTGGTLAGVAEYLRAQDPRVRIGLADPGGAALYN
                     HYAHGELRVTGESITEGIGQSRLTGNLEGFTPDFACQVTDAEALPCVFDLLQHEGLCL
                     GGSSAINIAGAVRLAREMGPGHTIVTILCDNGTRYQSKLFNPSFLRRKNLPVPAWLD"
     gene            complement(5591..5902)
                     /locus_tag="PLW13_11260"
     CDS             complement(5591..5902)
                     /locus_tag="PLW13_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY70999.1"
                     /translation="MIVSATLRKALKLAPALAAAALLAAPGSASASTTSGDAFGICRA
                     KVRATFGEEARTKLKRIKERGSFIVEIKVSGVAEKPFTAKCEVARDGTLSSFEHDGPA
                     K"
     gene            complement(6071..6436)
                     /locus_tag="PLW13_11265"
     CDS             complement(6071..6436)
                     /locus_tag="PLW13_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71000.1"
                     /translation="MKSPVALLVLLLIVFAPPPPAADALPLFDGLGSYSRTIATASPE
                     AQRYSDQELAFMYAFDHDEAIRACTRAIEADRRYTARAADRARDRCRHPDQIAVPVSA
                     GRPDVRPRSFVRAGFIKIG"
     gene            complement(6532..7536)
                     /locus_tag="PLW13_11270"
     CDS             complement(6532..7536)
                     /locus_tag="PLW13_11270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HQY71001.1"
                     /translation="MSNETSDHTGHPFDTLTPDAVIDAIESTGRTSDLRIFALNSYEN
                     RVYQVGIEGGAPLIVKFYRPERWSEEAIREEHDFTRELLEAELPVVAPMADERGDTLL
                     RFGEFRFALFPRVGGHVPDLDQPEQLLSLGRILGRMHAIGASRPFAHRPRVDIESYAE
                     TSWRLIAAHFIPPDLEAPYRSLCEALVQKLRERFRPDEATLIRTHGDLHPGNILARDG
                     ALCLVDLDDCRSAPAIQDLWMLLSGDRHERLAQLAEIVDGYEEFNDFPGAELPLVESL
                     RTLRLMHHAAWLAARWHDPAFPRYFPWFNTPRYWSEHILELREQLAALDEEPLALQRW
                     "
     gene            7734..8675
                     /locus_tag="PLW13_11275"
     CDS             7734..8675
                     /locus_tag="PLW13_11275"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY71002.1"
                     /translation="MSRFPFPIPYGWFHVGYSAELSSGEVRAVRYFGRDLALWRDQDG
                     NAHLQDAYCPHLGANIAVGGKVVGNLIECPFHKWRFTGEGRVAEIDYAQRLNDKACLA
                     SYPVQERHGVVMAWYHPQGVAPLYQLPDVPECSNPEFVGPFSTGHTIRTCLQEMAENT
                     ADAAHFVTIHQHPGQAHYDEFSFDGASMIMKSTQKFPSSHGPVEGTLNTDTAGFGFAI
                     VRYKTLVEICMVTTTAPVDAETSQQHNHVWYRNPARDPKIDRIGQAFVKEVNRQFTDD
                     IPIWENKIYVDKPNLCDGDGPIARFRKWAQQFYVGAA"
     gene            complement(8657..>9120)
                     /locus_tag="PLW13_11280"
     CDS             complement(8657..>9120)
                     /locus_tag="PLW13_11280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY71003.1"
                     /translation="RRLTEECERREREGSPLALAVCDIDNFKRVNDTFGHAAGDAVIV
                     AVAATLRAALRDDHFVARYGGEEFVLLLPGLGSPEAAQDLERLRAALESVPVAHAEQR
                     ITVTGSFGLAEFHPGDSAERVFMRADAALYRAKSGGRNRVCVYEDDQAAPT"
BASE COUNT         1379 a         2992 c         3263 g         1486 t
ORIGIN      
        1 gcagcatttc ttcgtcaacg gtgcgatggg taccaacagc ttcttcggca tggtcgagct
       61 caatccgttc gaggagcgcg agcgcggggc gcaggaggtc cagagcgacc tgaccacgcg
      121 gctcgcgggc gtgagcggcc tgcgcatcaa cgcgttcaac atgccctcga taccgggcgc
      181 ctcgcccgac ctgccgatca agttcgtggt ctcgggcacc ggctcctacg agttgctgtt
      241 ccaggtggcc caggacatcg agcgcaaggc gcgcgagagc ggcctgttcg tgtacgccga
      301 gtcggagctc aggttcgaca agcccgaggt gcggctggac atcgaccgcg acaaggcggc
      361 gcagctgggc atcgacgttg ccgccatcgg cagcacgctc tcgacgttcc tgaacgagaa
      421 cgagtcctcg cgcctcagcc tggacagccg cagctacgag gtgatcctgc agctcgagcg
      481 cgagtaccgg ctctctgccg acatgctcga cacctattac ctgcgcaccg gcagcggtga
      541 gctggtgccg ctgtccgccg tggtgtcgca ggcgcgcgag aaccggccca acaagctcac
      601 cgagttccag cagctcaatt ccgcgaccgt cggcgcgctg atggccccgg gggtgtcgct
      661 ggggcagggc atcgcgtggt tcgaggaaac cggccgcagc ctgctgccgg cgggctttcg
      721 cgccgattac gtcggccagt cgcgccagtt catgaccgaa ggcaacgtgc tgctgttcac
      781 cttcctgttc tccttcgtgc tgatcttcct ggtgctcgcg gcgcagttcg agagcttcag
      841 cgatccgttg accgtgctgg tgagcgtgcc gctgtcggtg tgcggggcgc tggtgccgct
      901 cgcgctcggc atggccacga tgaacatcta cacccaggtc ggcctgctga cgctgatcgg
      961 gctgatcagc aagcacggca tcctgatcgt ggacttcgcc aaccgccagc aggagatccg
     1021 cgatgccggc gcggtcgatg cggtgatcga ggccgcctcg gtgcggctgc gcccgatcct
     1081 gatgaccacg gcggcgatgg tcttcggcgt catgccgctg ctgttcgcca gcggcgccgg
     1141 cgcccaggcg cgggtctcga tcggcatcgt gatcaccttt ggcatgctgg tgggcacgct
     1201 gttcacgctg ttcgtggtgc cggtgattta cagctacgtg gcacggcggc acgtgcgcat
     1261 ggtcgacgac caggtggtgc gcaagcgcga tctttcctga gcgcccgggc aagcggcgcg
     1321 catttcggct atacatgctg acgtcctccg acgcgcggcc catatgggtc ggcgcgccgg
     1381 gtggttgacg acaaaatgac accgcaatca tgcgattcgc cttgggtctc gggagacgat
     1441 ttgttatcaa aaacaatgat atacggaaca tttttcatac tgtttttaca tggcatcatt
     1501 cttgattcaa gctcctcgca cagcgcaagg agcacgacaa aatgacgccg gacatcttca
     1561 agcccctgca caacgcgatc atctgggcgc agcactgtgc cgacccgctg atctggcgcg
     1621 aagcggtgcg gcgtttcgac gcggccgacc gcgcgctcgg cggactgccc tgccgcgagc
     1681 gcctccatct ggaggagcgc ctgtacggca tgctgccgag cgactggccg ctgtggatgg
     1741 agagttgcca ccagtcgatg agcgccatgc agcgccgcgc gctgacccac tgagcgcgcc
     1801 cgtttccgcc cggcggcttc cccgcggggc gcgcggctat actcgcgttc cacgggagac
     1861 gcgatgcaag agagcacggg agttcagcgc ctgctgcgcg gtgcgcgttt gtgcagcggg
     1921 ccactggcgg cggcactgtg gttcgccctc ggcgccagcg ccatggcgca cgatgcgcgc
     1981 gtggtgctcg ggctcgcgat ctggatggcg ttgtggtgga tgaccgaagc ggtgccgctt
     2041 gccgccaccg cgctgctgcc gctggtcatc ctgccgctgt tcacttcgat cggcttcggc
     2101 gccgccgcgg cgccctacgc cagcaacatc gtgttcctgt tcatgggcgg cttcatgctg
     2161 gggctggcgc tgcagcgctg cgggctgcac cggcgcatcg cgctggcgat gctggtcacg
     2221 gtgggctcgg gacagcgccg cctggtcggc ggcttcatgc tggtcaccgc gctgctgtcg
     2281 atgtggctgt cgaacaccgc cacgatgatg atgctggccc cgatcgggat cagcgtgctg
     2341 cagacccgcc gcgagcagcg ccccggcgcc gaggcggacg gcagcgaggc tttcggcgcg
     2401 gcgctgatcc tcggtatagc ctacgcggcc tcgatcggcg gcatgggcac cccgatcggc
     2461 acgcccccga acctcatcat gtccggttac ctgcgtgcgc actacggcat cgacgtcacg
     2521 atgctcgggt ggatgcgcgt cggcgtgccg gtgatcctgc tgctgttgcc gctggcctgg
     2581 ctctggttgt gcttcggggc cttccgcctg ccggcggccg gtttcgccgg cgacgaggcg
     2641 caactgcgcg cccagcgcgc gcggttgggc gcggcgagcg ccgcagagcg gcgcaccggc
     2701 gcggtgttcc tgctggtagc gctgggctgg gtgctgcgcc cgcaactggt ggcctggttc
     2761 ggtcttcccg gcctcgatga cggcgtgatc gcattgatcg gcgcactgct gctgttcgtg
     2821 ctgcccgcgc agggtctgga aggcgagcgc ctgctcgact ggaacaccgc gcgcggcatt
     2881 ccctgggaca tcctgctgct gttcggcggc ggcttgagcc tggccgcggc gctcacggcc
     2941 tcgggcgccg atgcgccgat cgcgcagtgg gcgtcggggc tcggatccgt gccggtggcg
     3001 ctgatcgtgg gcgtgatcgc cgtggcgatc gtgttcaccg gggaactgac cagcaatacc
     3061 gcggccgcca cggctatcat gccagtgctt gcggggctgg ccagcgcgcg cggcatcgat
     3121 cccttgccgc tgttcatggt cgccacgctg gccggtagct gcggtttcat gctgcccgtg
     3181 gcgacaccgc cgaatgcgat tgcctatggc accggattcg tgccgctgcg ggcgatgttg
     3241 cgggccggct tcgggctgaa cctgatcgct gtcgcggtgg tggtcggctt cgtgtggttc
     3301 gtggcgttct gaaggagttc cgatgactgt actgcccggc tgggagcgtt ccccggcctt
     3361 cgtgttcgac gccgcgcgct gcgcgctgtc cgtgctcgac caggcgcacc tgccgttcga
     3421 gcagcgctac ctgcagcccg gctcggtggc ggattgcgcg cgggcgatcc gcgacatgaa
     3481 cgtgcgcggc gcgccgttga tcgggctggt ggcggcagcc ggcatggcct tcgccgcgcg
     3541 cgccgacgcg agcgacgccg cgctcgaagc ggcggccgcg ctgctgatcg cgagccggcc
     3601 gaccgcggtc aacctgcgct gggccgtgga ggacctgcag gcggtgctgc gcaccgcgcc
     3661 gctggccgcg cgtgccgcgg cagccgccgc ccgggtgctg gcactgcagc actccgaatc
     3721 cgcatgctgc gcgcgcatcg gcgaacacgg cctcgggatc atgcgcgcgc tcgccgacac
     3781 gccccgggtg cgcgaacgcg gggtgctgaa cgtgctcacg cactgcaacg ccggctggct
     3841 ggcgaccggc gcctggggca cggcactggc gccggtgtac cgcgccgcgg ccgcgggtat
     3901 cgcggtgcac gtctgggtgg acgagacccg cccgcgcaac cagggggcgc ggctcaccgc
     3961 gtgggaactc ggcgagggcg gcatcgcgca cagcatcgtg gcggacaact gcgccgggca
     4021 cctgatgcag cgtggcctgg tggatgtctg catcgtcggc agcgaccgca ccacgcgcgc
     4081 gggcgacgtg tgcaacaaga tcggcacgta ccagaaggcg ctggcggccg ccgacaacgg
     4141 ggtgccgttc tacgtggccc tgccgtgctc gaccatcgac tggacgatta ccgacggcgt
     4201 gcgcgagatc gagatcgagg agcgcgacgc gagcgaggtg ctgcagatcg agggcgcggg
     4261 cgccgacggc gtgctgcgca gcgtgcgcca cgcgccgctg gcgagccggg cggcgaactt
     4321 cgccttcgac gtgacgccgg cgcggctggt gaccgggctg atctgcgagc acggcgtgtt
     4381 cgcggccgat gcgacggcgc tggcgccgtt gcgcgccgtg gccggcgctg caccggagcg
     4441 ggtatagttg cgcccgggtt gaaacctccg gggagcggca tggagaacag cagcggactg
     4501 gcggacctga tcggcaacac gccgctggtt cgcctgcgtc aggtgtcgga acagacgggg
     4561 tgcgagatcc tcggcaaggc agaattcatg aacccgggcg gctcggtcaa ggaccgcacc
     4621 gcgctcggca tcatccgcga cgccgagcgc cgtggcgtgc tgcgccaggg cgggcgcatc
     4681 gtcgagggca cggcgggcaa caccggcatc gggctgagcc tgctggccaa tgcgctcggc
     4741 tatcgcacca cgatcgtgat acccgagacc cagagccccg agaagatcga gatgctgatg
     4801 ctctgcggcg ccgagctgcg gctggtgccg gccgtgcccg ccagcgatcc caaccattac
     4861 gtgaagatct ccgggcggct ggcgcaggaa tacgcggcgc gcgaaacgca gggtgcggtc
     4921 tgggccaacc agttcgacaa caccgccaac cgcgcgatcc accgcgacac caccgggcgc
     4981 gagatctggg agcagacgcg cgggcgcgtc gatggcttca tctgctcggt gggcaccggt
     5041 ggcaccctcg ccggcgtggc cgagtacctg cgcgcgcagg atccgcgtgt gcgcatcggt
     5101 ctcgcggatc ccggcggcgc ggcgctgtac aaccattacg cgcacggcga gctgcgcgtg
     5161 acgggagaat cgatcaccga gggtatcggg cagtcgcgcc tgacgggcaa cctcgagggc
     5221 ttcacgcccg attttgcctg ccaggtgacg gacgccgagg cgctgccctg cgtcttcgac
     5281 ctgttgcagc acgaggggct ctgcctggga ggttcctcgg cgatcaacat cgccggtgcc
     5341 gtgcggctcg cgcgcgagat ggggcctggc cacaccatcg tgaccatcct gtgcgacaac
     5401 ggcacgcgct accagagcaa gctgttcaac ccgtcgttcc tgcggcgcaa gaacctgccg
     5461 gtgccggcgt ggctggactg aaaatcgtga agggtatgag gtcgggggcg ggcagagcgt
     5521 cacctgattg cccggcgtcg gggaaggcga acggggccgg cgcgctggca gcgccccggc
     5581 ccccggcggc ttacttggcg gggccgtcgt gctcgaagga gctcagcgtg ccgtcgcgtg
     5641 ccacttcgca cttcgcggtg aaaggcttct cggcaacgcc ggacaccttg atttccacga
     5701 tgaagctgcc gcgctccttg atgcgcttga gcttggtgcg ggcttcctcg ccgaaggtcg
     5761 cgcggacctt cgccctgcag atgccgaaag catcgccgga ggtggtcgag gcgctggcgc
     5821 tgccgggggc agcgagcagg gcggcggcgg ccagggccgg tgcgagtttc agtgctttac
     5881 gcagggtagc ggagacgatc attggcaggt agtcctcgag ttattgggca ataacgcccg
     5941 atggcatggg caaaagtgcc ctgaacccgg gaggttaatg cgctactcgg gaaaacgcac
     6001 tgaaagaatg gcgtaatttt caacaaaact gggtactttc gtaacagggc gcgccccgtt
     6061 tgggggcgca ttacccaatc ttaatgaagc cggcgcgaac gaatgagcgc gggcggacgt
     6121 ctggacgccc ggcagacaca ggcacggcga tttgatccgg atgtcggcaa cgatctcgcg
     6181 cacggtctgc agcgcgcgcg gtgtagcgac ggtcggcctc gatggcgcgc gtgcaggcgc
     6241 ggatcgcctc gtcgtggtcg aaggcataca tgaacgccag ctcctggtcg gaatagcgct
     6301 gcgcctcggg cgaagccgtg gcgatggtgc gcgaatacga gccgaggccg tcgaacagcg
     6361 gcagcgcatc ggcggcgggt ggcgggggcg cgaacacgat gagcagaagc acgagcaggg
     6421 caacagggga cttcatggca gtgctcctcc gtgtgcggga gtgatcgtgc gcggggggat
     6481 gcgccatcca tacgcccgcc acggccgcga tgccagcgca tcgcgccgcc gtcaccagcg
     6541 ctgcagcgcc agcggttcct cgtccagggc ggccagctgc tcgcgcagct ccaggatgtg
     6601 ctccgaccag tagcgcggcg tgttgaacca cgggaagtag cgcgggaagg ccgggtcgtg
     6661 ccagcgcgcc gccagccagg cggcgtggtg catcaggcgc agggtgcgca gcgattcgac
     6721 cagcggcagc tcggcgcccg ggaagtcgtt gaattcctcg tagccatcga cgatctcggc
     6781 gagctgcgcg aggcgctcgt gacgatcgcc gctgagcagc atccacaggt cctgtatcgc
     6841 cggggcgctg cggcagtcgt cgagatccac caggcacagc gcgccgtcgc gcgccaggat
     6901 gttgcccggg tgcaggtcgc cgtgggtgcg gatcagggtc gcctcgtccg ggcggaagcg
     6961 ctcgcgcagc ttctggacca gtgcctcgca caggctgcgg tagggcgcct cgaggtccgg
     7021 cggaatgaag tgcgcggcga tcaggcgcca gctcgtctcg gcgtagctct cgatatcgac
     7081 gcgcgggcgg tgcgcgaaag ggcggctggc gccaatcgcg tgcatgcgcc cgaggatgcg
     7141 cccgagcgac agcagctgct cgggctggtc gaggtcgggc acgtggccac cgacgcgagg
     7201 gaaaagcgcg aagcggaact cgccgaagcg cagcagggtg tcgccgcgct cgtcggccat
     7261 cggggccacg acgggcagtt cggcctcgag cagctcgcgc gtgaagtcgt gctcttcgcg
     7321 gatcgcctct tcgctccagc gctccgggcg gtagaacttc acgatcagcg gcgcaccgcc
     7381 ctcgatcccg acctggtaga cgcggttctc gtagctgttc agcgcgaaga tgcgcaggtc
     7441 cgaggtgcgc cccgtgctct cgatggcatc gatcacggca tcgggcgtca aggtgtcgaa
     7501 ggggtgcccg gtgtggtcgg aagtctcgtt gctcatggcg ggccggtact gctcagggcg
     7561 ggacagggta cgggcccgca ggtccgtcca caagggcggg ctccagatta caaaaccgtc
     7621 gtgttgcaat ctttttttga cactgtgggg gcgcgatccc tagaatgctc cggacacggc
     7681 gccgggcgct tgggcccgcc gctcgaccat gcaccgcagc agagggcaaa cgcatgagcc
     7741 gctttccgtt tccgattccc tatggctggt tccacgtcgg gtattcagcg gagctctcgt
     7801 cgggggaggt ccgcgcggtg cgctatttcg gccgcgacct cgcgctgtgg cgcgaccagg
     7861 acggcaacgc gcacctgcag gacgcgtact gtccgcacct gggcgccaac atcgcggtcg
     7921 gcggcaaggt ggtcggcaac ctgatcgagt gcccgttcca caagtggcgc ttcaccggcg
     7981 aggggcgtgt cgcggagatc gactatgcgc agcgcctgaa cgacaaggcc tgcctggcga
     8041 gctacccggt gcaggagcgt catggcgtgg tgatggcgtg gtatcacccg cagggggtcg
     8101 caccgctcta ccagctgccc gacgtacccg agtgcagcaa tcccgagttc gtggggccgt
     8161 tcagcaccgg ccatacgatc cgtacctgcc tgcaggagat ggcggagaac accgccgacg
     8221 ccgcgcactt cgtgaccatc caccagcatc cgggccaggc gcactacgac gagttcagct
     8281 tcgacggcgc gtcgatgatc atgaagagca cgcagaagtt cccgagctcg cacggaccgg
     8341 tggaaggaac gctcaacacc gataccgcgg gcttcggctt tgcgatcgtg cgctacaaga
     8401 cgctggtcga gatctgcatg gtcaccacca cggcgccggt ggacgcggag acctcgcagc
     8461 agcacaacca cgtctggtac cgcaacccgg cgcgcgatcc gaagatcgac cgcatcggcc
     8521 aggccttcgt gaaggaagtg aaccgccagt tcaccgacga catcccgatc tgggagaaca
     8581 agatctacgt cgacaaaccg aacctctgcg atggcgacgg cccgatcgcg cgctttcgca
     8641 agtgggcgca gcagttctac gtcggcgcgg cctgatcgtc ctcgtagacg cacacgcggt
     8701 tgcggccccc gctcttggcg cgatacagcg cggcgtcggc gcgcatgaac actcgttcgg
     8761 cggagtcgcc ggggtggaat tcggcgaggc cgaaggagcc ggtgaccgtg atgcgttgct
     8821 ctgcgtgcgc caccggcacg ctctccagcg cggcgcgcaa tctttccagg tcctgcgcgg
     8881 cctcgggtga gccgagtccc ggcagcagca ggacgaattc ctcgccgcca tagcgggcga
     8941 cgaagtgatc gtcgcgcagc gcggcacgca gggtcgcggc caccgcgacg atcacggcgt
     9001 cgccggccgc gtgcccgaag gtgtcgttga cgcgcttaaa attgtcgata tcgcacaccg
     9061 ccagcgccag cggcgagccc tcgcgctcgc ggcgctcgca ctcttcggtc agccgtcgtt
//
LOCUS       DAORUQ010000129         9039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8338_length_9039_cov_5.844056, whole genome shotgun sequence.
ACCESSION   DAORUQ010000129 DAORUQ010000000
VERSION     DAORUQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8338_length_9039_cov_5.844056"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..546)
                     /locus_tag="PLW13_11285"
     CDS             complement(40..546)
                     /locus_tag="PLW13_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71004.1"
                     /translation="MAIKTFGIDHIHVNVRSMDRFRDLMKQLFDLDATRIGHIDSIQA
                     DNATVRLRGTGAGQPFLDVFESGNPRSPINRLVEKRGPCVSFISFRVENIEEAAAHAT
                     RCGLQEISRVGFAGVEKQVQYNTMELLGFNLEFVEHEPGYAAAIEDIQRRLAAGEPVD
                     GIGASREF"
     gene            complement(613..1335)
                     /locus_tag="PLW13_11290"
     CDS             complement(613..1335)
                     /locus_tag="PLW13_11290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY71005.1"
                     /translation="MRRDLRPYWVKRVYLLFRSWYAEHFLRPQFDYLGDNGTYMKPWY
                     VDVNGPNVRLGHCATVIGDVDKRVNIAVWGRGPGQGRITIGDYALITPGVRISASDSI
                     EIGDNCMFASGAYITDSDWHGIYDRIERNAEPAPIRIGNNVWVGDRATVLKGVTIGDN
                     SIVGAGAVVARDVPANVVVAGNPARVVKELDPNGPFVTRADFYRDPTSMFAESDRLDR
                     EFLAGNTFFGWLRALFFPTTRD"
     gene            1474..2292
                     /gene="cysE"
                     /locus_tag="PLW13_11295"
     CDS             1474..2292
                     /gene="cysE"
                     /locus_tag="PLW13_11295"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQY71006.1"
                     /translation="MPIPPAKPHSPDEIWLSIRASAERAAREEPMLASFLHATILNHD
                     TLEAALSFHLAQKLDSPAAPALLVREVIEQAFASDPSIGAAIRCDLRAVNERDSACPN
                     HAVAFLFFKGFHALESYRISHWLWQQQRRPLALFLQNRISSEFGVDIHPAARVGRGIL
                     LDHATGVVIGETAVVGDNVSIMQAVTLGGTGKESGDRHPKVEAGVLIGAGAEILGNIR
                     IGEGAKVSAGSVVLADVAPHTTVAGVPAKVVGKPCSEQPALDMNHALSPDMANV"
     gene            complement(2554..3615)
                     /gene="hemE"
                     /locus_tag="PLW13_11300"
     CDS             complement(2554..3615)
                     /gene="hemE"
                     /locus_tag="PLW13_11300"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HQY71007.1"
                     /translation="MAQLKNDRLLRALRREPVDRTPVWIMRQAGRYLPEYRATRARAG
                     GIMELFRSPELACEVTLQPLDRFDLDAAILFSDILTVPDALGLGLYFEAGEGPRFRNP
                     VRSAADIAALPELDPERDLAYVMDAVRTIVKALDGRVPLIGFSGSPWTLATYMVEGGS
                     TRDFARIKTLAFTDPARMHQLLALLADAVTRYLEAQIRAGAQAVQVFDTWGGVLGHAA
                     YREFSLQYMTRIVAGLRAGVGRDIPVILFTKGGGLWIEDIAATGCDCIGLDWTADLGA
                     ARRHTGARVALQGNMDPAILRAPPERIRAEVAALLDSFGPGSGHVFNLGHGITPEIDP
                     EHVRVLVEAVRELSPAYHR"
     gene            complement(3776..5293)
                     /locus_tag="PLW13_11305"
     CDS             complement(3776..5293)
                     /locus_tag="PLW13_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY71008.1"
                     /translation="MPQEDWYLHYGLDRDPFAEAGVQGLFYPGGARQESIEQLQHLAR
                     FGDCVLLVTAAPGAGKSATIEHFVAQCAPDTRCAVVEVALLDGPEQILRRILAGFGMA
                     AEGSANLAHDLQRLELFCAQCHDEGVLSWLVFDDAQHLHVDALGLLASLLERTGGRLR
                     LLFFAEPGWEAVLREVLPGTVVHVIGLPAFDRDETLAYIHYRMKTAGLEAEPPFNASE
                     LEHIHLWSGGLPGRINGHARQALIEALGVVQRPLSTLPVWHFGVVAVTLLALLLLYAW
                     SAFDDDGAEDAVPRPGPVAGVVAAPEPVILAEEPAPDAAGGMDVAQAQAEPVDTDVAE
                     EAPAPEQAAADSRAASGPVGAPPPAATDVEQPPAVEMKQEVPALPPAERAPPPAQVQA
                     PVAAPPPAPGAGSLAADEDYLLSLDGSMYVLQIMGSDDAAQVRRFATRQSLALRQYSK
                     MNAGKRWYALVHGEFSDRAAAERAARDIAVQLPGTQPWLRRLDAVQGEIRQGRAR"
     gene            complement(5311..6408)
                     /gene="aroB"
                     /locus_tag="PLW13_11310"
     CDS             complement(5311..6408)
                     /gene="aroB"
                     /locus_tag="PLW13_11310"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQY71009.1"
                     /translation="MPAEILEVDLGERSYPIYIGSGLLDDAGLLLRHVPRGRIAVVSN
                     TTVAPLYLERVRAALGERLAVVKILPDGEQYKTLETLASIYDALLEARCDRHTTVLAL
                     GGGVVGDMAGFAAATYQRGVPFLQVPTTLLAQVDSSVGGKTGVNHARGKNMIGAFHQP
                     CCVIADTDTLATLPEREYAAGLAEVIKYGLIGDRPFLDWLRANVAALRRRDSAPLTEA
                     IRRSCANKARVVALDEREEGARALLNFGHTFAHAIETATGYSRFLHGEAVAIGMVMAA
                     ELSARMGWLAASDVPMVRELLLAAGLPVDPPAGLGEEQFLGLMAADKKVAAGKLRLVL
                     LRAPGEATVTADFPFELLRGLLADFLRRPAA"
     gene            complement(6511..7029)
                     /gene="aroK"
                     /locus_tag="PLW13_11315"
     CDS             complement(6511..7029)
                     /gene="aroK"
                     /locus_tag="PLW13_11315"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HQY71010.1"
                     /translation="MESSRNVVLVGPMGAGKSTIGRALADELGVVFQDTDSLIEERSG
                     ASIAWIFDVEGEEGFRDRETATIRDLAASRGAVIATGGGAVLREENRRLLRAMGAVVY
                     LETSVKQQLSRTRRDRKRPLLRSGDREEVLRELLALRDPLYREIADLVVRTDGRGAKS
                     VVQEIMEFLRAP"
     gene            complement(7040..>9039)
                     /gene="pilQ"
                     /locus_tag="PLW13_11320"
     CDS             complement(7040..>9039)
                     /gene="pilQ"
                     /locus_tag="PLW13_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HQY71011.1"
                     /translation="TPPSPQSYTIDKPARIALDLPGVSSALEQKKHTLAFDNAQSVVV
                     VESGGRTRVIVNLVEVAPYQASVEGNELVVSVGSGASGQYLTDAGGSVGATMGAASRA
                     GVHSGIKDFNFRRGANGEGRIELQLADPKADVDVRLEGNMIKVSFFDTTVPAELQRRY
                     DVVDFATPVEMIDVSQSGSQASVGIKVTGEYDYLAYQADTTYVVAVKPLTPQEAEAKK
                     KEFEFTGEKLSLNFQDIEVRAVLQLIADFTDLNLVASDTVTGRITLRLQNVPWDHALD
                     LILKAKGLDKRQVGNVLMVAPAAEIAERERQEIETNRQLEELAPLQTEFIKIKYANAQ
                     DLFKLFGNRGSQNVATGASQSAGSDANRNQREASILSPRGQVIVDERTNSLMVTETAE
                     KLEELRRLIKLIDVPIRQVLIEARIVVASTDFSQQIGVEWGGAGKDEDGNKIWSIGGS
                     QNTLAEINNGEDITFPDALAVDLGVTGQGTSSIAIGFTSNDLLLNAELSALEASGNGE
                     IVSQPKVITGDKQQATIRQGTEVPYQTSSASGETTVEFKDAVLQLDVTPHITPDGRVL
                     MKLLINQDSVGEFINGEFGSQIPTIDTTALNTEVLVGNGETVVLGGVFQTEEIVSQTK
                     TPVLGDIPYVGRLFRKDVTETSKNETLIFITPRILSEKLID"
BASE COUNT         1349 a         3169 c         3105 g         1416 t
ORIGIN      
        1 gcgggcagag ccgctcgccc cggctcgcgg gcacgggcat cagaattccc gggaggcgcc
       61 gatgccgtcc acgggttcgc cggcagccag gcgccgctgg atgtcctcga tggcggcggc
      121 gtagcccggc tcgtgctcga cgaactccag gttgaagccc agcagctcca tcgtgttgta
      181 ctgaacctgc ttctctacac cggcgaagcc cacgcgggag atctcctgca gcccgcatcg
      241 cgtcgcatgc gcggctgcct cctcgatgtt ctcgaccctg aaggagatga aggaaacgca
      301 cgggccacgc ttctccacca gtcgattgat gggactccgg ggattcccgg attcgaacac
      361 gtcgaggaac ggctggccgg cgccggtgcc acgcaacctg accgtggcgt tgtcggcctg
      421 gatggaatcg atgtggccga tgcgtgttgc atcgaggtcg aagagttgct tcatcagatc
      481 gcggaaccgg tccatgctcc gcacgttgac gtggatgtga tcgatgccga aggtcttgat
      541 tgccactgcc ggtgcctctg gtgcttgcga tggatacgga gtgcttcggt accgctgcgc
      601 cagcgcggca cctcaatcac gcgtggtcgg gaagaacagg gcgcgcaacc agccgaagaa
      661 ggtgttgccc gcgaggaact cgcggtccag ccgatcggac tccgcgaaca tcgaggtggg
      721 gtcgcgatag aagtcggcgc gtgtcacgaa cggcccgttc ggatccagct ccttcacgac
      781 gcgcgcgggg ttgcccgcga ccacgacgtt ggccggcacg tcgcgcgcca ccaccgcacc
      841 ggcgccgacg atgctgttgt cgccgatggt cacgcccttc agcacggtgg cgcgatcgcc
      901 gacccacacg ttgttgccga tgcggatcgg cgccggctcg gcgttgcgct cgatgcggtc
      961 gtagatgccg tgccagtcgg aatcggtgat gtaggcgccg ctcgcgaaca tgcagttgtc
     1021 gccgatctcg atcgagtcgc tggcgctgat gcgcacgccc ggggtgatca gcgcgtagtc
     1081 gccgatcgtg atccgcccct gcccggggcc gcgcccccac accgcgatgt tgacgcgctt
     1141 gtcgacgtcg ccgatcaccg tggcgcagtg gccgagccgc acgttcgggc cgttgacgtc
     1201 gacgtaccag ggcttcatgt aggtgccgtt gtcgccgagg tagtcgaact gggggcgcag
     1261 gaagtgctcc gcgtaccagc tgcgaaagag caggtatacc cgcttgaccc agtagggcct
     1321 cagatcccgg cgcatgcgat tccccccgtg agattcaggc cgccagcata catgcccgca
     1381 ccggcccgcc gcgaggcgcg acgaccgatg ctttgtcatg ccgcgcccgc tcggctacgc
     1441 tgtgcttcct cgccgatccc gccggagaca ccgatgccga tcccgcccgc caagccgcat
     1501 tcgccggacg agatctggct gtcgatccgc gcctcggccg agcgtgccgc gcgcgaggag
     1561 ccgatgctcg cgagctttct gcacgcgacc atactcaacc acgacacgct ggaggcggcg
     1621 ctgagcttcc acctcgcgca gaagctcgac agccccgccg cgccggcgct gctggtgcgc
     1681 gaggtcatcg agcaggcctt cgcgagcgat ccttcgatcg gcgcggcaat ccgctgcgat
     1741 ctgcgcgccg tgaacgaacg cgactcggcc tgccccaacc acgccgtggc cttcctgttc
     1801 ttcaagggct tccacgcgct cgagtcctac cgcatctcgc actggctgtg gcagcagcag
     1861 cgccgcccgc tcgccctgtt cctgcagaac cggatttcct ctgaattcgg cgtcgacatc
     1921 cacccggcgg cgcgcgtcgg gcgcggcatc ctgctcgatc acgccaccgg ggtggtgatc
     1981 ggcgagaccg cggtggtcgg cgacaacgtc tcgatcatgc aggcggtgac gctgggaggc
     2041 accggcaagg aaagcggcga ccgccacccg aaggtcgagg cgggcgtgct gatcggcgcc
     2101 ggcgcggaaa tcctcggcaa catccgcatc ggcgagggcg ccaaggtcag cgccggcagc
     2161 gtggtgctcg ccgacgtggc gccgcacacc accgtcgccg gcgtgcctgc aaaggtcgtc
     2221 ggcaagccct gcagcgagca gcccgcactg gacatgaacc acgcgctgtc gccggatatg
     2281 gcgaacgtct gagcgctctt gcgactgttg cgcatcgctt tcaacaggtc tgatgcgagc
     2341 gtccggcgcg cgagccactg cccctgccgc ggacacggcg taaatacgtc cctgtagcct
     2401 ctctgtcgcc atccatggcg acagaaggtc cgcggcgggg gcagtggctc gcgcgcctcg
     2461 ccttggtgga tcccggaatc gaacgtattc ggcaagccgc agcggcggca ccacctggcc
     2521 ggcgacgctc cagcctgacg attctgatcc acctcagcgg tgataagccg gcgacagctc
     2581 gcgcacggcc tcgaccagca cgcgcacgtg ctcggggtcg atctccggcg tgatgccgtg
     2641 gccgaggttg aagacgtggc cggagcccgg cccgaaggaa tcgagcagcg cggcgacctc
     2701 ggcgcggatg cgctcgggcg gggcgcgcag gatcgcggga tccatgttgc cctgcagcgc
     2761 cacgcgcgcg ccggtgtggc ggcgtgccgc gccgaggtcg gcggtccagt cgagaccgat
     2821 gcaatcgcaa ccggtggcgg cgatgtcctc gatccacaat cccccgccct tggtgaacag
     2881 gatgaccggg atgtcgcgcc cgacgccggc gcgcagcccc gcgacgatgc gcgtcatgta
     2941 ctgcagcgag aactcgcggt acgcggcgtg tcccagcacc ccgccccagg tgtcgaagac
     3001 ctgcaccgcc tgcgcgccgg cgcggatctg cgcctccagg tagcgcgtca ccgcatcggc
     3061 gagcagcgcc agcagctggt gcatgcgggc aggatcggtg aaagccagcg tcttgatgcg
     3121 cgcgaagtcg cgcgtcgagc cgccctcgac catgtaggtc gccagggtcc acgggctgcc
     3181 ggagaagccg atcagcggca cgcgaccgtc gagcgccttc acgatggtgc gcaccgcgtc
     3241 catcacgtaa gcgaggtcac gctcgggatc gagctcgggc agcgcggcga tgtccgcggc
     3301 gctgcgcacc gggttgcgaa agcgcggccc ctctccggcc tcgaaataga ggcccagccc
     3361 cagcgcatcg gggacggtga ggatgtccga aaacaggatc gccgcgtcca ggtcgaagcg
     3421 gtccagcggt tgcagcgtga cctcgcaggc cagctccgga ctgcggaaca gctccatgat
     3481 gccgccggcg cgggcacgcg tggcgcggta ctcgggcagg tagcgcccgg cctggcgcat
     3541 gatccagacg ggggtgcggt ccaccggttc gcggcgcagc gcgcgcagca ggcggtcgtt
     3601 cttcagctgt gccatggtca cctcggcgcg gccggggcga catgtgcgac cggccgatgc
     3661 atgcatccgg tccgcgggag gcgggccggt ggaaaaacag ggggcgattc tagcggtcgg
     3721 ggcgcgtggc cacaaaccgg cgtccgcgca agcgcgatgc aggtttcacg ggctctcagc
     3781 gcgcgcggcc ctggcggatc tcgccttgca cggcgtcgag acggcgcagc cacggctggg
     3841 tccccggcag ttgcaccgcg atgtcgcgcg ccgcgcgctc ggctgccgcg cggtcgctga
     3901 actcgccgtg caccagcgca taccagcgtt ttcccgcgtt catcttgctg tactgcctta
     3961 gcgccagcga ctggcgagtc gcgaagcgcc ggacctgcgc cgcatcgtcc gagcccatga
     4021 tctgcagcac gtacatcgag ccatccaggg acagcaggta gtcctcgtcc gcggcaagcg
     4081 agcccgcgcc aggcgcgggc ggcggcgccg ctacgggggc ctggacctgc gccggcggcg
     4141 gcgcgcgctc cgcagggggc agcgccggca cctcctgctt catctcgacg gccggaggct
     4201 gttccacgtc ggtagccgcc ggcggcggtg cgccaacggg gccggacgcg gcccgggagt
     4261 ccgccgcggc ctgttccggg gcgggcgcct cctcggcgac gtcggtgtcc acgggctctg
     4321 cctgcgcctg cgcgacgtcc atcccgccgg cggcatccgg cgccggctcc tcggcgagga
     4381 tcacgggctc cggcgcggcg acgacacccg ccaccggccc cgggcgcggc accgcgtcct
     4441 cggcgccgtc gtcatcgaag gcgctccagg cgtagagcaa cagcagtgcc agcagcgtga
     4501 cggccaccac gccgaaatgc cacaccggca gcgtcgacag ggggcgctgc accacgccca
     4561 gcgcctcgat gagcgcctgg cgggcatggc cgttgatgcg ccccggcagg ccgcccgacc
     4621 acaggtggat gtgctcgagc tcggaggcgt tgaacggcgg ctccgcctcg agtcccgcgg
     4681 tcttcatgcg gtagtggatg tacgccaggg tttcatcgcg gtcgaacgcg ggcagcccga
     4741 tcacgtgcac cacggtgccc ggcagaacct cgcgcagcac ggcttcccac cccggctcgg
     4801 cgaagaacaa cagccgcagc cgcccgcccg tgcgctcgag caacgacgcc agcaggccaa
     4861 gcgcgtccac gtgcaggtgc tgcgcatcgt cgaacacgag ccagctcagc accccctcgt
     4921 catggcattg cgcgcagaag agctccaggc gctgcaggtc gtgcgcgagg ttggcgctgc
     4981 cctcggccgc catgccgaag ccggcgagga tgcgccgcag gatctgctcg gggccgtcga
     5041 gcagcgccac ctcgaccacg gcgcagcgcg tgtccggggc gcactgggcc acgaaatgct
     5101 cgatcgtcgc cgacttgccg gcgccgggcg ccgcggtgac cagcagcacg caatcgccga
     5161 atcgcgccag gtgctgcaac tgctcgatgc tctcctggcg cgcgccgccg ggatagaaca
     5221 ggccctgcac cccagcctcg gcaaagggat cgcggtcgag gccgtaatgc aggtaccagt
     5281 cttcctgggg catccgcgcc tcccgtgcgc tcaggccgcc ggccggcgca ggaagtccgc
     5341 gagcaggccg cgcagcagct cgaacgggaa atccgcggtg accgtggcct cgcccggcgc
     5401 gcgcagcagc accagccgca gcttgccggc ggcgaccttc ttgtcggcgg ccatcagccc
     5461 gaggaactgc tcttcgccga gcccggcggg cggatccacc ggcaggcccg cggcgagcag
     5521 cagctcgcgc accatcggca cgtcggaggc cgccagccag cccatgcgcg ccgaaagctc
     5581 cgccgccatg accatgccga tcgcgacggc ctcgccgtgc aggaagcggc tgtagccggt
     5641 cgcggtctcg atcgcgtggg cgaaggtgtg gccgaagttc agcagcgcgc gcgcgccctc
     5701 ctcgcgctcg tccaacgcca ccacgcgcgc cttgttggcg caggaacggc ggatcgcctc
     5761 ggtcagtggc gcgctgtcgc gtcgacgcag tgccgcgacg ttggcgcgca gccagtcgag
     5821 gaacggccgg tcgccgatca gcccgtactt gatcacctcc gccagccccg cggcgtattc
     5881 gcgctcgggc agcgtcgcga gcgtgtcggt gtcggcgatc acgcagcagg gctggtggaa
     5941 ggcaccgatc atgttcttgc cgcgcgcatg gttcacgccg gtcttgccgc ccaccgagga
     6001 gtcgacctgc gccagcagcg tggtgggcac ctgcaggaag ggtacgccgc gctggtaggt
     6061 cgcggccgcg aagcccgcca tgtccccgac cacgccgccg ccgagcgcca gcacggtggt
     6121 gtgacggtcg cagcgcgcct cgagcagcgc atcgtagatg gaggcgagcg tctcgagggt
     6181 cttgtactgt tcgccgtcgg gcaggatctt caccaccgcg agccgctcgc ccagcgccgc
     6241 gcgcacgcgc tccaggtaca gcggcgccac cgtggtgttg ctcaccacgg caatgcggcc
     6301 gcggggaacg tggcgcagca gcagtccggc gtcatcgagc aggcccgaac cgatgtagat
     6361 cgggtagctg cgctcgccga ggtcgacttc gagaatttca gcaggcatgg aggcttctgg
     6421 cagctgttga gcggagccgc cgcccggcgc gctccccggg cgaagcttaa cggcgaatgc
     6481 gccgggccac cagtcagccg gtgcacggcc tcagggcgcg cgcaggaatt ccatgatttc
     6541 ctgcaccacg gacttggcgc cgcgtccgtc cgtgcgcacc accaggtcgg cgatctcgcg
     6601 atacagcggg tcgcgcagcg cgagcagttc gcgcagcacc tcctcgcggt cgcccgagcg
     6661 cagcagcggg cgcttgcggt cgcgccgcgt gcgcgagagc tgctgcttca cggaggtctc
     6721 aagatagacc acggcgccca tggcgcgcag caggcggcgg ttctcctcgc gcagcaccgc
     6781 gccgccgccg gtggcgatca ccgcgcccct gctggcggcg agatcgcgga tcgtcgctgt
     6841 ttcacgatcg cggaagcctt cctcgccctc gacgtcgaag atccacgcga tgctcgcgcc
     6901 cgagcgctcc tcgatcagcg aatcggtgtc ctggaagacc acgccgagct cgtcggcgag
     6961 cgcgcgaccg atggtgctct tgccggcccc catggggccg acgagcacca cgttcctcga
     7021 agactccatc ccggcgcggt cagtcgatca gcttttcgct gaggatgcgc ggcgtgatga
     7081 agatcagcgt ctcgttcttg ctggtctcgg tcacgtcctt gcggaacagg cgcccgacgt
     7141 aggggatgtc gcccagcacc ggggtcttgg tctggctgac gatctcctcg gtctggaaca
     7201 cgccgcccag caccacggtc tcgccgttcc ccaccagcac ctcggtgttg agcgccgtgg
     7261 tgtcgatggt cgggatctgc gaaccgaact cgccgttgat gaattcgccc accgagtcct
     7321 ggttgatcag cagcttcatc agcacccggc cgtccggcgt gatgtgcggc gtcacgtcga
     7381 gctgcagcac cgcgtccttg aattccacgg tggtctcgcc cgaggcagaa gaggtctggt
     7441 acggaacctc ggtaccctgc cggatggtgg cctgctgctt gtcgccggtg atcaccttgg
     7501 gctgcgaaac gatctcgccg ttgccggagg cctccagggc ggagagctcg gcgttcagca
     7561 gcaggtcgtt ggaggtgaag ccgatcgcga tggagctggt gccttggccg gtgacgccga
     7621 ggtcgaccgc gagcgcgtcg gggaaggtga tgtcctcccc gttgttgatc tccgccagcg
     7681 tgttctggct gccgccaatg ctccagatct tgttgccgtc ttcgtccttg ccggcgccgc
     7741 cccattccac gccgatctgc tgcgagaaat cggtggaggc gaccacgatg cgcgcctcga
     7801 tcagcacctg gcggatcggc acgtcgatca gcttgatcag gcggcgcagt tcctcgagct
     7861 tctcggcggt ctcggtgacc atcagcgagt tggtgcgctc gtcgacgatg acctgtccgc
     7921 gcggcgacag gatgctcgcc tcacgctggt tgcggttcgc atcgctgccg gcggactgcg
     7981 aggcgccggt ggcgacgttc tggctgccgc ggttgccgaa cagcttgaac aggtcctgcg
     8041 cattggcgta cttgatcttg atgaactcgg tctgcagcgg cgccagttcc tcgagctggc
     8101 ggttggtctc gatctcctgg cgctcgcgct cggcgatctc ggcggccggt gcaaccatca
     8161 gcacgttgcc gacctggcgc ttgtcgaggc ccttggcctt gaggatcagg tcgagcgcgt
     8221 ggtcccaggg cacgttctgc aggcgcagcg tgatgcgccc ggtcaccgtg tcgctggcca
     8281 cgaggttgag gtcggtgaaa tccgcgatca gctgcagtac cgcgcgcacc tcgatgtcct
     8341 ggaagttgag cgacagcttc tcgccggtga actcgaactc cttcttcttc gcctcggcct
     8401 cctggggcgt cagcggcttc accgccacca cgtaggtggt atcggcctgg taggcgaggt
     8461 aatcgtattc accggtgacc ttgatgccca cgctcgcctg cgaacccgac tggctcacgt
     8521 cgatcatttc caccggcgtg gcgaaatcga ccacgtcgta gcggcgctgc agttcggccg
     8581 gcacggtggt gtcgaagaac gacaccttga tcatgttgcc ctcgaggcgc acatcgacgt
     8641 ccgccttggg atcggccagc tgcaattcga tgcgaccctc gccgttggcg ccgcggcgga
     8701 agttgaagtc cttgatgccc gagtgcacgc ccgcgcgcga cgcagcgccc attgtcgcgc
     8761 cgacgctgcc gccggcatcc gtgaggtact gcccgctcgc gcccgagccg acactgacca
     8821 ccagctcgtt gccctcgacg ctcgcctggt aaggcgcgac ctcgaccagg ttcacgatca
     8881 cccgcgtgcg cccacccgac tcgacgacca ccacgctctg cgcgttgtcg aaggccagcg
     8941 tgtgcttctt ttgctcgagc gcgctcgata cgccaggcag gtcgagcgcg atgcgcgccg
     9001 gcttgtcgat ggtgtaggac tgcggcgacg gcggcgtgc
//
LOCUS       DAORUQ010000130         8724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8810_length_8724_cov_5.506517, whole genome shotgun sequence.
ACCESSION   DAORUQ010000130 DAORUQ010000000
VERSION     DAORUQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8810_length_8724_cov_5.506517"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLW13_11325"
     CDS             <1..606
                     /locus_tag="PLW13_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71012.1"
                     /translation="MNHPGLTVHAMYTMGGERTNGVFFDDVYVPDEYRVGELNRGFQY
                     IAEALDIERFTLFTFSPIRARMELLADYVKRTTRDGKPLREDPVIRQRIAQLATECEV
                     GRLLGVKFISAAMQGGKTPTVEASCYKLYATELSLRIANATMDIVGPGAQLALDTEDA
                     PLRGRPERCYTYTVIDTIGGGSSEVQKNIIARRHLGLPKNF"
     gene            622..1782
                     /locus_tag="PLW13_11330"
     CDS             622..1782
                     /locus_tag="PLW13_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQY71013.1"
                     /translation="MSFLQGFKVLDLASVGPAARASRILADYGMAVIKVAPVSRKGQK
                     QTEPVYHAYGAGRGTRRIRVDLESAEGRDTVYRLARASDVLIESFRPGVAQRIGVGYE
                     QLRTHNPRIVYCSTSGYGQHGPCAQWAGHDINYLAMGGFLACSGRDAAGRPAIPGATV
                     ADSAGGGMHAALAIVAALVNRLRCDEGAYLDVAITDGVLNLMSLHVDEYLATGVETGP
                     GSALLTGKYACYGVYATRDGGALAVGAIETRFFANLCRLLGVDALAAAQYDDARQDEL
                     KAALSEKFLARDRDAWTELLAGENTCVTPVLGVAEVAANAHFAARGSFVEARHPVHGA
                     FRQLAPVLAGNARECPPHQVAAAGATDTDAVLGDAGFDATEIARLRRDGVVE"
     gene            1818..2342
                     /locus_tag="PLW13_11335"
     CDS             1818..2342
                     /locus_tag="PLW13_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HQY71014.1"
                     /translation="MDEASNGIPAAVQALIGVAQHAEESSFPIEMAYVYNTCAAVQNG
                     NPLYWDEAVAREITHGWITPPTMMSVWFRPHYWVPGASGERLALQLHFDLKRLFDLPE
                     AIIAGNETVFGVPVRPGDRLRAHQVLRSVSAVKTTRVGVGRFWVIDLECQNQHGEFVG
                     RDSYDCFGYRRPAA"
     gene            2339..2827
                     /locus_tag="PLW13_11340"
     CDS             2339..2827
                     /locus_tag="PLW13_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQY71015.1"
                     /translation="MSIGELRILTLEQVREGDELPPLAHQVTATTVVLGALASRDWRP
                     MHHDREFAISRNGVRDIFINTPNNAAWFERYVTDWTGPRGRLGRMKFRMGEPVFPGDE
                     MILSGRVTRAAADDTGCGWVDLDLAVSVSGTVKTSCRARVAIPCDGDDNPWERKDERW
                     KP"
     gene            2879..4039
                     /locus_tag="PLW13_11345"
     CDS             2879..4039
                     /locus_tag="PLW13_11345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71016.1"
                     /translation="MDLNFTEEQTLLRDMVRNLCEEHASTRVVRDMENDPLGVPPGLW
                     TQMKDTGLLAMMLPEAYGGIGLDMLDRAVIYHEFGRALAPGPHFASSVMGALAIEKGG
                     SQAQKDALLTAIGQGGLIVAPAWLEPDNGFGPQGVQLRAEASADGYRLDGVKRHVFHA
                     RAAQKLIVLARTGEAPDAIDLLLVDTDAPGVTLEQQLSMASDTQYKVRFDGVRVPVAN
                     RLGAPQSGWATWEACMYEGIILLAAQAVGGAERALEITVEYSKGREQFDKPIGSFQAL
                     AHYMADAVAVIEGAATLVLEAAWAHTRGKSIARLAPMAKLFACNAFRDVTAKCEQIHG
                     GYGFTLEYDIQLFFRRAKQQQMNWWDSRYLEGLIAAEVLDGMGRTIEDPFAV"
     gene            4113..6575
                     /locus_tag="PLW13_11350"
     CDS             4113..6575
                     /locus_tag="PLW13_11350"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY71017.1"
                     /translation="MSAGALAGIKVIDFGQMVSAPFCAKLFADYGADVIKVELPAGDA
                     ARATGPFPGDVADPEKSGLYFINNTNKRGISCDVSTEAGRAVFLRLAAWADLLIENNA
                     PAQMRAWGLDYATLAAANPDLVVVSITPFGQTGPYSDWNGHDLNAYHLTGASSRYCGR
                     PEETPLEHGTFAADYFGAVAAAAWGLAAVHGRRQAGGGQHLDVSTAEAIAAAFVGGQN
                     IGGYAQDRVFDKRTGVGMPQGAPATIMPCKDGHVWMLALEPGQWNGLRKVMGDPEWAD
                     LELFQDMFQRARNADLIYSFIEEWTMQHGKMEIMEMCQAAGCPVTAVLTVAEAADQPH
                     LAARDFFVDIAHPVLGTVRTLGAPFKLPVSPGGPRTAAPLLGQHTGEVLREIAAPDGA
                     AIGTPARDGGEGRARAPRAEHQTVSALPLKGIRVINFGWVWAGPVAGQTLGFLGAEVL
                     KIESRARIDMTRNLPPFAEGVPGPDRSLSNHACWAGNGSVSLNLKQPQAKRLALELIA
                     KSDVVIENFGPGVMEQLGLGYAQLRKVKHDLVMFSMPAAGLYGPLREVRTYGLSVTST
                     TGLDSLVGYKGGGPVPVENAFSDPFVGIFGSFAILAALHHRDASGEGQHIDFSQQEAI
                     MQMVGPAYMDYVLNGRIGGPKGNEHPLAAAAPHGVFPCAGDDRWISIAVPTDAEWQAL
                     VRAMGGPEWATLDCFATSALRVAHIQELHAGLAAWTAGFDDRALAAMLQAHGVAAAPV
                     LNVADLLTDPHYRARGTFIEVRHPLGFRETIYGAYVKTSRTVPDVQPGPVIGRDNERV
                     FRDILGMPQERYRQLVEEKVIY"
     gene            6662..7552
                     /locus_tag="PLW13_11355"
     CDS             6662..7552
                     /locus_tag="PLW13_11355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY71018.1"
                     /translation="MKFALSVPGLLNYPPAMAPWEVRATGKDIIRFARHADGLSWDWL
                     TVPEHLFMPDGMVDVMGRRFPEGITACAVLAGATERIHMLTYVLVVPYRNPVLLAKQI
                     ATMEFIAGGRFTLGAAPGHLKREFEVLGIPFEERGWITDEYIRAMIECWTSDTPTFEG
                     KYVSFKGIVMEPKPVQKPYPPIFIGGNTEIAMKRAVRHGDGWIPWLITTDELRRCIDF
                     MSTQPGWEEKKDRFEIVMPTTEYKVEDYSHAEKGETEIVMDRDRILFELEELQKAGAT
                     VAQVMTPPVDAVDQCLEWIE"
     gene            7617..8447
                     /locus_tag="PLW13_11360"
     CDS             7617..8447
                     /locus_tag="PLW13_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY71019.1"
                     /translation="MAREDDMLLFRIEDGVGIITLNRPHKLNAISWDLAEELSRLLYL
                     LRDDDAVRVVLLHGAGRAFCAGGDLEFISGDSERPMPGTSDPSRPMPRSQRKSPGGPF
                     VDVVRQIVALDKPVIAAIHGAAVGAGLGYAMACDRRFGDTTTRMSAIFTNVGVSPDCG
                     VSWFLPRLVGLSNALMLVETAKILNAEESRAIGLLDELVPEGRAFEAALDYARLIARR
                     ASVAVDMARRLIYRGQTTTLEDILDYEAAVGTMVASAFDAREGATAFLEKRTPVFRGN
                     "
     gene            complement(8512..>8724)
                     /locus_tag="PLW13_11365"
     CDS             complement(8512..>8724)
                     /locus_tag="PLW13_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71020.1"
                     /translation="SARDKMAARKKQALAVSVADLGLTDEQLTPGVVLAKQYLPEIHG
                     HCEFIEGTPADAAAALIARLRADKLI"
BASE COUNT         1453 a         2849 c         3103 g         1319 t
ORIGIN      
        1 atgaaccacc cggggctcac ggtgcacgcg atgtacacga tgggcggcga gcgcaccaac
       61 ggcgtgttct tcgacgacgt ctacgtcccc gacgagtatc gcgtcggcga actgaaccgg
      121 ggcttccagt acattgccga ggcgctcgac atcgagcgat tcacgctgtt caccttctcg
      181 ccgatccgcg cgcgcatgga attgctcgcc gattacgtca agcgcacgac gcgcgacggc
      241 aagccgctgc gcgaggatcc ggtgatccgc cagcgcatcg cgcaactggc gaccgagtgc
      301 gaggtcggcc gcctgctggg cgtcaagttc atcagcgccg cgatgcaggg cggcaagacg
      361 ccgaccgtcg aggcgtcctg ctacaagctc tacgcgacgg aactgtcgct gcgcatagcc
      421 aacgccacga tggacatcgt cgggccgggg gcgcagctcg cgctggacac cgaggacgcg
      481 ccgctgcggg ggcgtccgga gcgctgctac acgtacacgg tgatcgacac catcggcggt
      541 ggcagttccg aggtgcagaa gaacatcatc gcgcgccgtc atctcggcct gccgaagaac
      601 ttctgagcag ggagtgccgc gatgtccttc ctgcaggggt tcaaggtgct cgacctggcc
      661 agcgtgggtc cggcggcgcg cgcctcgcgc atcctcgccg actacggcat ggcagtcatc
      721 aaggtcgcgc cggtctcgcg caaggggcag aagcagaccg agccggtcta tcacgcctat
      781 ggcgcgggac gcggcacgcg taggatccgc gtcgacctgg aatcggccga gggccgtgac
      841 accgtctacc gcctcgcgcg ggcgagcgac gtgctcatcg agagcttccg tcccggcgtc
      901 gcgcagcgca tcggcgtcgg gtacgaacag ctgcgcacgc acaacccgcg catcgtgtac
      961 tgctcgacca gcggctacgg gcagcacggt ccgtgcgcgc aatgggcggg tcatgacatc
     1021 aactatctcg cgatgggtgg ctttctcgcc tgcagcggcc gcgatgcggc gggtcgtccc
     1081 gcgatccccg gcgccacggt ggccgacagc gccggaggcg gcatgcacgc agcgctcgcg
     1141 atcgtcgcgg cgctggtgaa ccggctgcgc tgcgacgagg gggcatatct cgacgtcgcg
     1201 atcaccgacg gcgtgctcaa cctgatgtcg ctccacgtcg acgaatacct cgcgaccggt
     1261 gtcgaaacgg gaccggggag cgcattgctc accgggaaat atgcctgtta cggcgtctac
     1321 gccacgcgcg atggcggcgc gctcgcggtg ggcgcgatcg agacgcgctt cttcgcgaac
     1381 ctgtgccgcc tgctcggcgt ggacgcgctg gccgcggcgc agtacgacga cgcgcggcag
     1441 gacgaactga aagcggcatt gagcgagaag ttcctcgcgc gcgaccgcga cgcatggacc
     1501 gagctgctcg cgggcgagaa cacctgcgtg accccggtgc tgggagtagc cgaggtggcc
     1561 gcgaatgccc atttcgcggc gcgcggcagc ttcgtcgagg cgcggcaccc ggtgcatggc
     1621 gcctttcgcc agctcgcacc cgtgctggcc ggcaacgcgc gcgaatgccc gccgcaccag
     1681 gtcgccgccg cgggagccac cgataccgat gccgtgctgg gcgatgcggg cttcgatgcg
     1741 acggaaatcg cgcgcctgcg tcgtgacggt gtcgtggaat gaacgcaaca gcgccggacc
     1801 ggccggaggc aacggacatg gatgaggcga gcaacgggat tccggccgcg gtgcaggccc
     1861 tgatcggcgt cgcgcagcac gccgaggaat cgagcttccc gatcgagatg gcctatgtct
     1921 acaacacctg tgccgcggtg cagaacggca atccgctgta ctgggacgag gcggtcgcgc
     1981 gcgagatcac gcacggctgg atcacgccgc cgacgatgat gtccgtgtgg ttccggccgc
     2041 actactgggt gccgggggcg agcggcgagc gtctggcgct gcaattgcat ttcgacctca
     2101 agcgcctgtt cgacctgccc gaggcgatca tcgccggcaa cgaaaccgtt ttcggcgtgc
     2161 cggtgcgtcc cggcgaccgg ctgcgcgccc accaggtgct gcgctcggtc agcgcggtga
     2221 agaccacgcg cgtcggtgtg ggccggttct gggtgataga cctcgagtgc caaaaccagc
     2281 atggcgagtt cgtgggccgc gactcctacg actgcttcgg ctaccggagg cccgcagcat
     2341 gagcatcggc gagctcagga tactgaccct ggagcaggtc agggagggcg acgagctgcc
     2401 gccgctggcg caccaggtca ccgcgaccac ggtggtactg ggcgcgctcg cgagccgcga
     2461 ctggcgcccg atgcaccacg accgcgaatt cgcgatcagc cgcaacggcg tcagggacat
     2521 cttcatcaac acgcccaaca acgccgcgtg gttcgagcgc tacgtcaccg actggaccgg
     2581 cccgcggggt cggctcgggc gcatgaaatt ccgcatgggc gagcccgttt ttcccggcga
     2641 cgagatgatt ctctccgggc gtgtgacgcg cgctgcggcg gacgacacgg gctgcggatg
     2701 ggtggatctc gacctggcgg tatccgtgtc gggcacggtc aagacctcgt gccgggcgcg
     2761 cgtggcgatt ccgtgcgacg gggacgacaa cccgtgggaa aggaaggacg agcgctggaa
     2821 gccctgaggg gatgccggca cccgatggtt gcacacgatt gagcgagggg cgacagccat
     2881 ggatttgaac ttcaccgaag agcagacgct tctgcgcgac atggtgcgca acctctgcga
     2941 ggagcacgcc agcacgcgcg tcgtgcgcga catggagaac gatcccctcg gcgttcctcc
     3001 cggcctgtgg acgcagatga aggacaccgg cctgctggcg atgatgcttc ccgaggcgta
     3061 cggcggcatc ggcctcgaca tgctggaccg cgcggtcatc taccacgagt tcggccgcgc
     3121 gctggccccg ggtccgcact tcgcgagctc ggtaatgggc gcgctggcga tcgagaaagg
     3181 cggcagccag gcgcagaagg acgccctgct cacggcgatc ggccagggag gcctgatcgt
     3241 cgcgccggcc tggctcgagc ccgacaacgg cttcggtccg caaggcgtgc agctgcgtgc
     3301 cgaggcgagc gccgacggct accggctcga cggcgtgaag cggcacgtgt tccacgccag
     3361 ggcggcgcag aagctcatcg tgctggcgcg cacgggcgag gcgcccgatg cgatcgacct
     3421 gctgctcgtc gacacggacg cgccgggcgt cacgctggag cagcagctgt cgatggcctc
     3481 ggatacccag tacaaggtcc ggttcgacgg agtccgggtg ccggtcgcga accggctcgg
     3541 cgcgccgcag tcgggctggg cgacctggga ggcatgcatg tacgagggga tcatcctgct
     3601 ggccgcgcag gcggtgggtg gtgccgagcg cgcgctggag atcacggtcg aatactccaa
     3661 gggccgggag cagttcgaca agccgatcgg ctccttccag gcgctggccc actacatggc
     3721 ggatgcggtc gcggtcatcg agggcgctgc aaccctggtg ctcgaggctg cctgggcgca
     3781 cacgagggga aaatccatcg cgcgcctggc gccgatggcg aagctgttcg cgtgcaacgc
     3841 ctttcgcgat gtcaccgcca agtgcgagca gattcacggc ggctacggct tcacgctgga
     3901 gtacgatatc cagctcttct tccgccgcgc caagcagcag cagatgaact ggtgggattc
     3961 gcgttatctc gagggcctga tcgccgccga ggtactcgac ggaatgggcc gcaccatcga
     4021 ggacccgttc gcggtgtgag cgtcctggcc gcctgggcgc ggcgtatggc ggtggacaag
     4081 gggcgggagt gcgcaattcg ggaggtgggc acatgagcgc cggagcgctg gcgggcatca
     4141 aggtcatcga tttcgggcag atggtctccg cgccgttctg cgcgaaactg ttcgccgact
     4201 acggtgccga cgtgatcaag gtcgaactgc ccgcgggcga tgccgcgcgc gcgaccgggc
     4261 cgtttccggg cgatgtggcg gatcccgaga agagcgggct ctacttcatc aacaacacca
     4321 acaagcgcgg catcagctgc gacgtgagca ccgaggcggg gcgggcggtg ttcctgcgtc
     4381 tcgcggcgtg ggcggatctg ctgatcgaga acaacgcgcc ggcgcagatg cgcgcatggg
     4441 ggctcgatta cgccacgctg gccgccgcga acccggacct cgtcgtggtc tcgatcacgc
     4501 cattcggcca gaccggtccc tacagcgact ggaacggcca cgacctcaac gcgtaccacc
     4561 tcaccggcgc gagcagccgc tactgcgggc gcccggagga aacgccgctc gagcacggca
     4621 cctttgcggc ggattacttc ggcgcggtcg cggcggcagc ctggggactg gcagcggtcc
     4681 acggtcggcg gcaggcgggc ggcgggcagc acctggacgt gtccacggcc gaggccatcg
     4741 cggccgcgtt cgtgggcgga cagaacatcg gtggctacgc gcaggaccgc gtcttcgaca
     4801 agcgcacggg cgtgggcatg ccgcagggcg cgccggcgac gatcatgccc tgcaaggacg
     4861 gccacgtctg gatgctggcg ctggagccgg ggcagtggaa cgggttgcgc aaggtcatgg
     4921 gcgatccgga atgggccgat ctcgaactgt tccaggacat gttccagcgc gcccggaacg
     4981 ccgacctgat ctattcgttc atcgaggaat ggacgatgca gcacggcaag atggaaatca
     5041 tggagatgtg ccaggcggcg ggttgcccgg tgacggccgt gctcacggtg gccgaggccg
     5101 cggaccagcc gcacctcgcc gcgcgcgact tcttcgtcga catcgcccat cccgtgctgg
     5161 gaaccgtgcg cacgctgggg gcgcccttca agctgccggt ctcgcccggt ggcccgcgca
     5221 cggcggcgcc gctgctcggg cagcacaccg gggaggtgct gcgcgagatc gccgcaccgg
     5281 acggcgcagc aatcggcacg ccggcgcgcg acggcgggga aggccgggcg cgcgcgccgc
     5341 gcgcggaaca tcagacggtc agcgcattgc ccctgaaggg catccgggtg atcaatttcg
     5401 gctgggtctg ggcgggcccg gtggccgggc agacgctggg ctttctcggc gccgaggtgc
     5461 tcaagatcga gtcgcgcgcg cgcatcgaca tgacgcgcaa cctgccgccc ttcgccgaag
     5521 gcgtgccggg ccccgaccgc agcctgtcca accacgcctg ctgggccggc aacggctcgg
     5581 tatcgctgaa cctgaagcag ccgcaggcga agcgactggc gctcgagctg atcgcgaaat
     5641 cggacgtggt gatcgagaac ttcggtcccg gcgtgatgga gcagctgggc ctcggctacg
     5701 cgcaactgcg caaggtcaag cacgacctgg tgatgttctc gatgcccgcg gcgggcctct
     5761 acggaccgct gcgcgaggtg cgcacctacg ggctcagcgt cacctcgacc accggcctgg
     5821 acagcctcgt cggctacaag gggggcggtc cggtcccggt ggagaacgca ttctcggatc
     5881 ccttcgtcgg gatcttcgga tcctttgcca tcctggctgc gctgcatcat cgcgacgcca
     5941 gcggcgaggg gcagcacatc gatttctcgc agcaggaagc catcatgcag atggtgggac
     6001 ccgcgtacat ggactacgtg ctgaacggac gcatcggcgg accgaagggc aacgagcacc
     6061 cgcttgcagc ggctgcgccg cacggcgtgt tcccgtgcgc cggcgatgat cgctggatca
     6121 gcatcgcggt gccgaccgat gccgagtggc aggcgctggt gcgcgcgatg ggcggccccg
     6181 agtgggcgac actggactgc ttcgcgacat cggcgctgcg ggtggcgcac atccaggaat
     6241 tgcatgcggg gctcgccgcc tggaccgccg gcttcgacga ccgcgcgctc gccgcgatgc
     6301 tgcaagcgca cggggtcgcg gcggcgccgg tgctcaatgt ggcggacctg ctcaccgacc
     6361 cgcactaccg ggcgcgcggc accttcatcg aggtgaggca tccgctgggt ttccgcgaaa
     6421 ccatctacgg cgcctacgtg aagaccagtc gcacggtgcc ggacgtgcag ccggggcccg
     6481 tgatcgggcg cgacaacgag cgcgtgttca gggacatcct cggcatgccg caggagcgct
     6541 accggcagct cgtggaggaa aaggtcatct attagcggca gcgccgcgcg gtcgtggcgg
     6601 cgcgatgcct ccgcgaccgg caggcaacgc caatccgatc gccgagtcga aggaatacat
     6661 catgaaattc gccttatccg tgcccggtct gctgaattac ccgcccgcga tggcgccgtg
     6721 ggaggtgcgc gcgaccggaa aggacatcat ccgcttcgcc aggcatgcgg acgggctgag
     6781 ctgggactgg ctgacggtgc cggagcacct gttcatgccc gacgggatgg tcgatgtgat
     6841 ggggcgacgc tttcccgagg gaatcacggc ctgcgcggtg ctggccggcg cgaccgagcg
     6901 catccacatg ctcacctacg tgctggtggt gccttaccgc aacccggtgc tgctcgccaa
     6961 gcagatcgcg acgatggagt tcatcgcggg cgggcgtttc acgctgggcg cggcgcccgg
     7021 tcacctgaaa cgcgaattcg aggtgctcgg gataccgttc gaggaacgcg ggtggatcac
     7081 ggacgagtac atccgcgcga tgatcgaatg ctggacctcg gacaccccca ccttcgaggg
     7141 caagtacgtg agcttcaagg gcatagtcat ggagcccaag ccggtgcaga agccctaccc
     7201 gccgatcttc atcggcggca ataccgagat cgcgatgaag cgcgccgtgc gacacggcga
     7261 cggctggatt ccatggctca tcaccacgga cgagctcagg cgctgcatcg acttcatgag
     7321 cacgcagccg ggttgggaag agaagaagga tcgcttcgag atcgtgatgc cgaccacgga
     7381 atacaaggtc gaggattatt cgcacgccga gaagggcgag acggagatcg tgatggatcg
     7441 cgaccggatc ctgttcgagc tcgaggaact gcagaaggcg ggtgccacgg tggcccaggt
     7501 catgacgccg cccgtcgatg ccgtcgacca gtgcctggaa tggatcgaat gatacgacag
     7561 cgacatcatt ccgaacttcc gctgatatcc gcacaacgac caggagggaa ttaccgatgg
     7621 cccgggaaga cgacatgctg ctgttccgga tcgaggacgg ggtgggcatc atcacgctca
     7681 accgtccgca caagctgaac gccatcagct gggacctggc cgaggagctc tcgcggctgc
     7741 tctacctgct gcgcgacgac gacgcggtgc gcgtggtgtt gctgcacggc gcggggcggg
     7801 ccttctgcgc cggcggcgat ctcgaattca tctccggtga cagcgagcgc ccgatgccgg
     7861 gtacctcgga tccgtcgcgt cccatgccgc gttctcagcg caagagcccc ggcggcccct
     7921 tcgtggacgt ggtgcgccag atcgtcgcgc tggacaagcc cgtgatcgcg gccatccacg
     7981 gcgcggcggt cggcgcgggg ctcggctatg ccatggcctg cgatcggcgc ttcggcgaca
     8041 ccacgacgcg gatgtcggcg atcttcacca atgtcggggt gtcgcccgac tgcggcgtgt
     8101 catggttcct cccgcgcctg gtcggcctgt ccaacgcgtt gatgctggtc gagacggcga
     8161 agatactgaa cgccgaggaa tccagggcga tcgggttgct ggacgaactc gtgcccgagg
     8221 gtagggcatt cgaggcggcg ctcgactacg cgcgcctgat cgcgcgccgc gccagcgtgg
     8281 cggtcgacat ggcgcgcagg ctcatctatc gcgggcagac caccaccctc gaggacatcc
     8341 tggactacga agcggcggtg gggaccatgg tggcctcggc cttcgacgcc agggagggcg
     8401 ccacggcctt cctcgagaag cgcacgcccg tcttccgcgg gaactgaggc gcgcgaatcc
     8461 tgcagccgcc ggtgcacatc ccgcaccggc cggcaacgaa tccacgggct ctcagatcag
     8521 cttgtcggcg cgcagcctgg cgatgagtgc ggcggccgca tcggcaggcg taccctcgat
     8581 gaactcgcaa tgcccatgga tctctggcag gtactgcttc gcgagcacca ccccgggcgt
     8641 gagctgttcg tccgtgaggc cgaggtcggc gacggacacc gcgagcgcct gcttcttgcg
     8701 tgccgccatc ttgtcgcgcg cgct
//
LOCUS       DAORUQ010000131         8699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8853_length_8699_cov_5.916705, whole genome shotgun sequence.
ACCESSION   DAORUQ010000131 DAORUQ010000000
VERSION     DAORUQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_8699_cov_5.916705"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLW13_11370"
     CDS             complement(<1..861)
                     /locus_tag="PLW13_11370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71021.1"
                     /translation="MTEWTLEGKTVLITGATGGIGLAAAVAMARQGAHLVLVGRNQTR
                     GLDAISEIRRRAGSDRAEFLRADFSSLAEVRRLALALLAEDRPLHVLVNNAGTMNTSR
                     KLTVDGFEEMFAVNHLAHFLLTRLLLPRLQACAPSRIVHVASNAHSFCKGMRWHDLSH
                     EREFSAFPAYGHSKLANILFSNELARRLRGSGVTSNALHPGAVATGIGSNNGITGRVV
                     PLLLKPFFRSPARGAATTVYLASAAEIDGVTGKYFYDSKPADPKPWALDTNAAGRLWV
                     TSEKLLGDLLA"
     gene            complement(904..1530)
                     /locus_tag="PLW13_11375"
     CDS             complement(904..1530)
                     /locus_tag="PLW13_11375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQY71022.1"
                     /translation="MTSATSPKIVAPALEDTIESCSRLADALRISDAPDAVLEDVRTR
                     IESVIDLLRAHARPGPHSQSVLNQHTAEFFSTHMLAPHDVMPYSPIIGRLNPVSPRLE
                     FHAVDNRIEGRGVFPVRFVGAPQTVHGGMVAMALDELMGLVNFLNGAGAFTGTMTVRY
                     HRPTPIERELTFLGEMVEADGRKVKSRAEIRCDGELMASAEGLFIRPR"
     gene            complement(2184..2483)
                     /locus_tag="PLW13_11380"
     CDS             complement(2184..2483)
                     /locus_tag="PLW13_11380"
                     /inference="COORDINATES: protein motif:HMM:NF039725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HQY71023.1"
                     /translation="MTDKRQTDTNTARSWFRSGSRVFVDRGQWYFNTREGTVEGPYAD
                     KQSAERFLESYVQVLSSKFVPGTEFSLMEDSAYDTPVARSADSPDLGLGRFIQAR"
     gene            complement(2690..2766)
                     /locus_tag="PLW13_11385"
     tRNA            complement(2690..2766)
                     /locus_tag="PLW13_11385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:tct)
     gene            3298..3513
                     /locus_tag="PLW13_11390"
     CDS             3298..3513
                     /locus_tag="PLW13_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71024.1"
                     /translation="MRQSIVIAVFAAGLLAGIIGSTLASDPGNRQISAAGEGAWILGA
                     NARVTYCWTRSNPTGSKEIDCTVSASP"
     gene            complement(3553..4356)
                     /locus_tag="PLW13_11395"
     CDS             complement(3553..4356)
                     /locus_tag="PLW13_11395"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQY71025.1"
                     /translation="MSFVKITRPRPQVALVTLDRPERMNAMAFDVMVPLREALEELSF
                     DNSVRVVVLTGAGDGFCSGADLVDPGYLSIFDGLTMPGISRRAMKVLDDVVKSLRDMH
                     QPVIAAINGPAIGGGFCLAMATDIRVAADEAYFRAAGINNGLTAAELGLSFTLPRAIG
                     STRAFEIMLSGRDVGAEEAERIGIVSQRCARAELLETCFGMAERIAGYSQVGVELTKQ
                     MLWAGLEAGSMHSHMNHEGHAQLFVRMTTRNFEEAIKARKDKRKPEFKD"
     gene            complement(4425..4985)
                     /locus_tag="PLW13_11400"
     CDS             complement(4425..4985)
                     /locus_tag="PLW13_11400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY71026.1"
                     /translation="MKFCSSCGGAISRRIPVNDDRERHVCDSCETVHYQNPRIVVGCL
                     PVFGEQVLLCRRAIQPRRDYWTLPAGFMENGETSLEGARRETWEEARARVEGESLYTI
                     FDLPHISQVYMFYLARLSAPEFAAGPESLEVALFDEDAIPWDELAFPVVNATLERFFA
                     DRRHGIFPVHNATLRPGDWGRGVRRS"
     gene            complement(4991..5467)
                     /locus_tag="PLW13_11405"
     CDS             complement(4991..5467)
                     /locus_tag="PLW13_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQY71027.1"
                     /translation="MSFDSLQLRNALGRFATGVCVITANPEGHAPFGMTVNSFSSLSL
                     TPPLVLWSLQKNSDTMEAFRVARQYCVNILSQEQKALSGRYAKKGDHALLAEEFTIGE
                     SGLPVLRHSLASFECDMDARHDGGDHVILVGRVTHMTVATGGRPLVFYEGGYRELA"
     gene            complement(5464..5940)
                     /gene="moaE"
                     /locus_tag="PLW13_11410"
     CDS             complement(5464..5940)
                     /gene="moaE"
                     /locus_tag="PLW13_11410"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF007959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HQY71028.1"
                     /translation="MQPEVEVREADFGIDEEYARLRGEPGSAGACVLFTGLVREWSDG
                     AQALELEHYPGMTEASIAAIVRDARARWQLGAVRVIHRVGRLAPGDQIVLVGVSSAHR
                     HEAFAACEFIMDYLKTRAPIWKKEIGTGGACWVESRDSDVLAASRWRESATNEEMT"
     gene            complement(5955..6206)
                     /gene="moaD"
                     /locus_tag="PLW13_11415"
     CDS             complement(5955..6206)
                     /gene="moaD"
                     /locus_tag="PLW13_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HQY71029.1"
                     /translation="MLRVLFFASIRERLGRRELALACDAACTSIAGLVARLESELPGC
                     AALLLAEKTIVAVNREVVTREQALRDGDEIAFYPPVTGG"
     gene            complement(6210..6692)
                     /gene="moaC"
                     /locus_tag="PLW13_11420"
     CDS             complement(6210..6692)
                     /gene="moaC"
                     /locus_tag="PLW13_11420"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQY71030.1"
                     /translation="MNSGLTHISDAGEARMVDVGAKPASARAAVAEAWVRMRADTLRL
                     IVEGGHHKGDVFAVARIAGIQAAKRCAELIPLCHPLGLDAADVQLEAVPEQSAVRIRV
                     ECRLHGRTGVEMEALTGASVAALTVYDMCKAVDKGMVVDGVRLLEKRGGRSGSWQAEA
                     "
     gene            6797..7879
                     /locus_tag="PLW13_11425"
     CDS             6797..7879
                     /locus_tag="PLW13_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71031.1"
                     /translation="MRAAASLLFALALLGGCGEKFPPVNGCVPAGGMNPTCSFSSPED
                     MELLPDGRTLLISQMGTADGRRPGSFALFDTGSETITRLPQFSASDEPPWGDAACTAA
                     PGAAFSPHGIHLGLRADGQWQVLAVNHGGRESVEFFQLLEESEGYRLAWRGCALAPPD
                     SYLNDVAALPGGGFVATHMFPRSSASIGPVSLALLRGMLGFDTGHVLHWDGQRFAVAT
                     GTRMPFPNGIQASRDGKALFVNAYIAGEVRKIGFPGGELLGKTSVKGPDNSQWDAEGH
                     LLVASHTAGIVEMGTCFGITEGACGAAFAIVAVDPGSLKAETILEHRGAPMGAATVAQ
                     QVGENLYLGSFVGDRIVRAPMPAQAR"
     gene            complement(7920..>8699)
                     /locus_tag="PLW13_11430"
     CDS             complement(7920..>8699)
                     /locus_tag="PLW13_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY71032.1"
                     /translation="PLWMTHGEYYVGRVFSQTGMEGPFWEGMDFYRRAGAAPEFLENI
                     RLRMSGFSGMVEPLPRSFHRVRGGDGFVIGGSRWEAVVGHGHSPEHLCLLNRERRLLI
                     SGDQVIPLITSNVSVMAIEPEGDPLADWLESHRRFLELPDDVLVLPAHNTPFYGLHAR
                     LRYLIAHHEDHLAALEEACVTPSTVRELLPVIFRRTLDAEQTTLALGECIAHMNFLLR
                     AGRVRCEPGADGLHRYIAPAPAVAARAGRVTHQRDDDPLMV"
BASE COUNT         1426 a         3035 c         2846 g         1392 t
ORIGIN      
        1 tgccagcaga tctcccagca acttctcact cgtcacccac aaccgccccg cggcattcgt
       61 atccagcgcc cacggcttcg gatccgcggg cttcgaatcg tagaagtact tccccgtgac
      121 gccgtcgatc tccgcggccg aggcgagata gaccgtggtc gcggcccccc tggcggggga
      181 gcggaagaac ggcttcagca gcaacggcac cacgcgcccg gtgatgccgt tgttggagcc
      241 gatgccggtc gcgaccgctc ccggatggag cgcattggaa gtcacgccgc tgccacgcag
      301 gcgccgcgcg agttcgttgg aaaacaggat gttcgcgagc ttcgagtgac cataggccgg
      361 gaacgcggaa aactcgcgct cgtgcgagag gtcgtgccag cgcatcccct tgcagaacga
      421 atgggcgttg gaggccacgt gcacgatgcg cgagggcgcg caagcctgca agcgcggcag
      481 caacaggcgt gtgagcagga agtgcgcgag gtggttgacc gcgaacatct cctcgaaacc
      541 gtcgacggtg agcttgcgcg aggtgttcat cgtcccggca ttgttgacga gcacgtgtag
      601 cggccggtcc tcggcaagca gggccagcgc gaggcgccgt acctcggcga gcgaggaaaa
      661 atccgcgcgc aggaactcgg cgcggtcgct gccggcgcgc cggcgtatct ccgagatcgc
      721 gtccaggcca cgcgtctggt tgcgcccgac cagcacgagg tgcgcgccct ggcgcgccat
      781 cgcgacggca gccgcgagcc ctatgccacc ggtggcgccg gtgatcagca ccgtcttccc
      841 ttccagcgtc cactcagtca ctgcggcacc tccacctggc gctctcggct gcaagcaagc
      901 ggttcaacgc ggccggatga acaggccctc ggccgatgcc atcagttccc cgtcacagcg
      961 gatctcggcg cggctcttca ccttgcggcc atcggcttcc accatctcgc cgaggaacgt
     1021 gagctcgcgc tcgatcggtg tcgggcggtg gtagcgcacc gtcatcgtgc cggtgaaggc
     1081 gcccgcgcca ttcaggaaat tcaccagccc catgagttcg tccagcgcca tcgcgaccat
     1141 gccgccgtgg acggtctgcg gcgcacccac gaaacgcacc gggaacaccc cgcgcccctc
     1201 gatgcggttg tcgacggcgt ggaactcgag ccgcggggac accggattga ggcgcccgat
     1261 gatcgggctg tagggcatca cgtcatgggg ggcgagcatg tgggtgctga agaactcggc
     1321 cgtatgctgg ttcagcaccg actgcgaatg cggtcccggg cgggcgtgcg cgcgaagcag
     1381 atcgatcacg ctctcgatgc gtgtacgcac gtcctcgagc accgcgtcgg gcgcgtcgct
     1441 gatccgcagc gcatcggcca ggcgcgagca actctcgatg gtgtcttcaa gcgccggggc
     1501 cacgatcttc ggggatgtcg cggatgtcat tgcatgcctc tgttctactg cttatgcgag
     1561 gcattatgac catcattacc gggagcggcc agagcgcacg aacgtcaggc agacgcaacc
     1621 ccggagatat tcgccgggat cgccgcgccg cgttaccgga gcagcactga tgccagcctc
     1681 ccgggcatcc ccctgccctc gtgaaggcgg ctagcggtct ttccgggtgc cgaaaaatcg
     1741 ctcgaaattg acatatcgcc gcagcagcca ccagcggctg gagcgggcgc tgtcacgttc
     1801 ggcacgccgg tctgtctgcc tgcgtttgtc ctctcccgga cccgctggcg cgtcgtcacg
     1861 cgaagcccgt cgatcggcgc cactgcgggg atcatcgaac attcggggct ccggcatacg
     1921 ggatggtgaa atcggtggtt acgccaggct ggccgtagcc catttgggct acgagcgccg
     1981 gtgtgcgagt tcaaattaga agatttcgat gcaattgcaa gttcgaaggg tcatcggccc
     2041 cgcgctggct agcaaccgct acgaactaca ttgggcactc gttggcgatc ctggatcagc
     2101 caacatgcgg gcggaccctg gacgaggggc ccaaagcaac ggtaacagtg gacgcgccgg
     2161 tggcgggccg cggtgtcgat cgtctagcga gcctggatga accgtccgag gcccagatca
     2221 ggcgaatccg cggagcgcgc aacgggggtg tcgtaagcgc tgtcctccat gagactgaac
     2281 tccgtgcccg gcacgaactt cgagctgagg acctgcacgt agctctcgag aaagcgttcg
     2341 gccgactgct tgtcggcgta gggaccctcg acggtgcctt cacgggtatt gaaataccac
     2401 tgtcccctgt ccacgaatac gcgcgacccg ctccggaacc atgaccgtgc cgtattggta
     2461 tcggtttgcc gtttgtcagt cattgacgta actcgactcc cacctgacgc taccgtgatt
     2521 ctaggcacgg ctgcaggctc gatcacgccc attgtgtgaa cttcatctca aaagaggagc
     2581 gtggccggtg cctgtgaaaa tgtcgggctc gggcggcggc gccgcacttc ggtatgcact
     2641 ctgcgcttcc cggtcaccga gtcgaaaagt cctaactaat tgatttgatt ggcgcgcccg
     2701 gagggactcg aacccccgac catccgctta gaaggcggat gctctatcca gctgagctac
     2761 gggcgcaaaa ctctctgatt ccacccaccg gcggagtcgc atcaagcggc aggcctgtcg
     2821 gcctgctgat gctccccgtc gccttgcggc gccgggtccg gccactcgcc ggcaggctcg
     2881 tggcgttcgc acgggccgac gcgatgcgtc ggctccctcc gtgctcaccc agctgagcta
     2941 cggacgcaaa actctctgat tccacccacc ggcggagtcg catcaagcgg caggcctgtc
     3001 ggcctgctga tgctccccgt cgccttgcgg cgccgggtcc ggccactcgc cggcaggctc
     3061 gtggcgttcg cacgggccga cgcgatgcgt cggctccctc cgtgctcacc cagctgagct
     3121 acgggcgcaa aggaaaaggc cgcattatag ccacaccggc ccccagtggc ccagccgaga
     3181 acggctgggc gcgccgtgca tggatcggga ggatgcgcta aggtcgcgcc ggcagcgtcc
     3241 cagcctctcg aatcctcacc tcgcacggga ctctatggat caacgaggag aatcatcatg
     3301 cgccaatcca tcgtcatcgc cgtcttcgcc gccggcctgc tcgccggcat catcgggtcc
     3361 accctggcct ccgatcccgg caatcgccag atcagcgccg ccggcgaagg cgcgtggatc
     3421 ctcggggcca acgcccgcgt gacctattgc tggacgcgct cgaatcccac gggcagcaag
     3481 gaaatcgact gcacggtttc cgcatcgccc tgagcgcgcg acgcaacgtc cgccacggca
     3541 cgcggcgggc gctcagtcct tgaattcggg cttgcgcttg tccttgcgcg ccttgatcgc
     3601 ttcctcgaag ttgcgcgtgg tcatgcgcac gaagagctgc gcgtggccct cgtggttcat
     3661 gtgcgagtgc atgctgcccg cctcgaggcc cgcccagagc atctgcttgg tgagttccac
     3721 cccgacctgg ctgtaaccgg cgatgcgctc ggccatcccg aagcaggtct ccagcagctc
     3781 cgcgcgcgcg cagcgctgcg acacgatgcc gatgcgctcg gcttcctcgg caccgacgtc
     3841 gcggccgctc agcatgatct cgaaggcccg cgtggaaccg atggcacgcg gcagcgtgaa
     3901 gctgaggccg agctccgcgg cggtgagccc gttgttgatg cccgcggcgc ggaaataggc
     3961 ctcgtcagcc gcaacgcgga tgtcggtggc catcgccagg cagaagccgc cgccgatcgc
     4021 cggaccgttg atcgcggcga tcacgggctg gtgcatgtcg cgcagggatt tcacgacgtc
     4081 gtccagcacc ttcatcgcgc gccgcgagat gccgggcatc gtgagcccgt cgaagatcga
     4141 gaggtaaccc ggatcgacca ggtccgcgcc cgaacagaag ccatcgcccg cgccggtcag
     4201 caccaccacg cgcacgctgt tgtcgaagct gagctcctcc agcgcctcgc gcagcggcac
     4261 catcacgtcg aaggccatcg cgttcatgcg ctcgggccga tcgagcgtga ccagcgcgac
     4321 ctgcgggcgg ggacgggtga tcttgacgaa ggacatgggc agggctccgg gatggcttcg
     4381 ccctagccta gctgctgcgc cggcacgcgc ccgttacggc actgtcatga tcggcgcacg
     4441 ccgcgacccc agtcgcccgg gcgcagcgtg gcgttgtgca ccgggaagat gccgtggcgg
     4501 cggtcggcga agaagcgctc caaggtcgcg ttcaccaccg ggaaagcgag ctcgtcccag
     4561 ggaatcgcgt cctcgtcgaa cagcgcgacc tcgaggctct cggggcccgc cgcgaattcg
     4621 ggtgcgctga ggcgcgcgag gtagaacatg tagacctggc tgatgtgcgg caggtcgaag
     4681 atcgtgtaca gcgactcgcc ctcgacgcgc gcccgcgcct cctcccaggt ctcgcgccgc
     4741 gcgccttcca gcgaggtttc gccgttctcc atgaagccgg ccgggagcgt ccagtaatcg
     4801 cgccgcggct ggatcgcgcg tcggcacagc agcacctgct cgccgaacac gggcaggcat
     4861 ccgaccacga tgcgcgggtt ctggtagtgg acggtctcgc agctgtcgca gacgtggcgc
     4921 tcgcggtcgt cattgacggg aatgcggcgc gaaatcgcgc cgccacagct gctgcagaac
     4981 ttcatcgggt tcaggcgagc tcgcggtagc cgccttcgta gaacaccagc ggccggccac
     5041 ccgtggccac cgtcatgtgt gtcacgcggc cgacaaggat gacgtgatcg ccgccgtcat
     5101 ggcgcgcgtc catgtcgcac tcgaaggacg cgagcgagtg cctcagcacc ggcagcccgc
     5161 tctcgccaat ggtgaactcc tccgcgagca gcgcgtgatc gcctttcttc gcgtagcggc
     5221 ccgaaagcgc cttctgctcc tgcgacagga tgttcacgca gtactgcctg gcgacgcgga
     5281 aggcctccat cgtgtccgag ttcttctgca ggctccacag caccagcggg ggcgtcagcg
     5341 acagcgagga aaaggaattc accgtcatgc cgaagggagc gtggccctcg ggattcgcgg
     5401 tgatcacgca cacgccggtg gcgaagcgcc cgagcgcgtt gcgcaactgc agactgtcga
     5461 aactcatgtc atctcctcgt tggttgcaga ttcccgccag cgcgacgcgg caagcacgtc
     5521 actgtcccgg ctctcgaccc agcacgcgcc acccgtgccg atttccttct tccagatcgg
     5581 ggcccgcgtc ttcaggtagt ccatgatgaa ctcgcacgcc gcgaacgcct cgtgacgatg
     5641 cgcgctgctc acgcccacca gaacgatctg gtcgcccggc gccagcctgc cgacacggtg
     5701 gatcacgcgc accgcgccga gctgccagcg cgcgcgcgca tcgcgcacga tcgcggcaat
     5761 gctcgcctcg gtcatgccgg ggtagtgctc gagctccagc gcctgagcgc catcgctcca
     5821 ttcgcgcacg agcccggtga acagcacgca ggccccggcg ctccccggct cgccgcgcag
     5881 ccgcgcatat tcctcgtcga tgccgaagtc cgcctcgcgg acctccacct cgggttgcat
     5941 cacgccgcct cccgtcaacc gccggtgacc ggcgggtaga acgcgatctc gtcgccgtcg
     6001 cgcaatgcct gctcgcgcgt gaccacctcg cggttcaccg ccacgatggt cttctcggcc
     6061 agcagcaacg cggcgcagcc cggcaattcg ctctccaatc gtgccaccag gccggcgatg
     6121 ctggtgcacg ccgcgtcaca ggccagcgcc agttcgcgtc ggccgagccg ctcgcggatg
     6181 ctggcgaaga acagcacgcg cagcatcgct caggcctcgg cctgccagga cccggaacgc
     6241 ccgccgcgct tctcgagcag gcgcacgcca tcgaccacca tgcccttgtc caccgccttg
     6301 cacatgtcgt agacggtcag cgccgccacc gaggcgcccg tcagtgcctc catctcgacg
     6361 ccggtgcgcc cgtgcagccg gcactcgaca cggatgcgca ccgcggactg ttccggcacc
     6421 gcctcgagtt gcacatccgc ggcatcgagg ccgagcggat ggcacagcgg aatcagttcg
     6481 gcgcagcgct tggccgcctg gatgcccgcg atgcgcgcaa ccgcgaacac atcgcccttg
     6541 tggtgtccgc cctcgacgat cagtcgcagc gtatcggcac gcatcctgac ccacgcctcg
     6601 gccaccgcag cgcgcgcgct agccggcttg gcgccaacat cgaccatgcg cgcctcgccg
     6661 gcatcgctga tatgggtcaa tccggaattc acacctgtca ccgtccgctt gcgcctgttg
     6721 ttcccgtgcc gggactggcc ggcatcgcag cggcacacta ccatagcggc gccagccacg
     6781 ggagtgagat aagaccatgc gcgccgccgc ctcgttgctg ttcgcgctcg cgctgctcgg
     6841 cggttgcggc gagaagttcc cgcccgtcaa cggctgcgtg cccgcgggcg ggatgaatcc
     6901 gacctgcagc ttcagcagtc ccgaggacat ggaactgctg cccgacgggc gcacgctgct
     6961 gatcagccag atgggcactg cggacggacg ccgtccgggc tctttcgcgc tgttcgacac
     7021 cgggagcgaa accatcacgc gcctgccgca gttcagcgcc tccgacgagc cgccgtgggg
     7081 agatgccgcc tgcaccgcgg cgcccggagc cgctttctcg ccgcacggca tccacctcgg
     7141 cctgcgtgcg gacggccagt ggcaggtgct cgcggtcaat cacgggggac gcgagagcgt
     7201 cgagttcttc cagttgctcg aggagagcga gggctaccgg ctggcgtggc gcggttgcgc
     7261 gctggcgccg cccgacagct acctcaacga cgtggcggca ctcccggggg gcggcttcgt
     7321 cgccacgcac atgttcccgc gcagcagcgc gtcgatcggg ccggtgagcc tggcgctgct
     7381 caggggcatg ctcggcttcg acaccggcca cgtgctgcac tgggacggtc agcgcttcgc
     7441 ggtggccact ggcacccgca tgcccttccc caacggcatc caggccagcc gcgacggcaa
     7501 ggcgctgttc gtcaatgcct atatcgcggg cgaggtgcgc aagatcgggt ttcccggcgg
     7561 cgaattgctc ggcaagacaa gcgtgaaagg cccggacaac agtcagtggg atgccgaggg
     7621 tcacctgctg gtggcctcgc acaccgccgg catcgtggaa atgggcacct gcttcggcat
     7681 taccgaggga gcctgcgggg cggcgttcgc gatcgttgcg gtggatccgg gcagcctgaa
     7741 ggcggagacc atcctcgagc accgcggcgc gccgatgggc gcagccacgg tggcccagca
     7801 ggtcggtgag aatctctatc tcggatcctt cgtcggtgac cgcatcgtgc gcgccccgat
     7861 gcccgcgcag gcacgctgaa cggcacgcta gcgctggcgc cgcgggccga gcggcccgtc
     7921 tagaccatca gcggatcgtc gtcgcgctgg tgcgtcaccc tgcccgcgcg cgcggccacc
     7981 gccggagcgg gcgcgatgta acggtgcagg ccgtcggcgc cgggttcgca gcgcacgcgc
     8041 ccggcgcgca gcaggaaatt catgtgcgcg atgcactcgc ccagcgccag ggtggtctgc
     8101 tccgcgtcga gcgtgcgcct gaatatcacc ggcagcagtt cgcgcacggt gctgggcgta
     8161 acgcacgcct cctccagcgc cgcaaggtga tcctcgtgat gggcgatcag gtagcgcagc
     8221 cgtgcatgca ggccgtaaaa cggcgtgttg tgcgccggca gcaccagcac gtcgtccggc
     8281 aattcgagga agcgccggtg cgactccagc cagtcggcga gcggatcacc ctcgggctcg
     8341 atcgccatca cgctgacgtt cgaggtgatc agcggaatca cctggtcgcc ggagatcagc
     8401 agcctgcgct cgcggttgag caggcacagg tgttccggcg aatggccgtg cccgacgacc
     8461 gcttcccagc gcgagccgcc gatgacgaat ccgtcgcccc ccctgacacg atggaacgag
     8521 cgcggcagcg gttcgaccat gcccgagaag ccgctcatgc gcaggcggat gttctcgagg
     8581 aattccggcg ccgcgccggc gcggcggtag aaatccatcc cttcccagaa tggcccctcc
     8641 atgccggtct gcgagaatac ccgcccgacg tagtattcgc cgtgcgtcat ccacagcgg
//
LOCUS       DAORUQ010000132         8681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8890_length_8681_cov_6.667517, whole genome shotgun sequence.
ACCESSION   DAORUQ010000132 DAORUQ010000000
VERSION     DAORUQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8890_length_8681_cov_6.667517"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLW13_11435"
     CDS             <1..722
                     /locus_tag="PLW13_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQY71033.1"
                     /translation="LLHNWALHPGTWRPDPSIMTEFDLWTFNSKVFPAIEPLVARTGE
                     RVRIRVGNLSMHEHPVHLHGVQFHVTGGDGGRLPQALWRTEVTELVGVGQMRDIEFVA
                     IAGDWALHCHKSHHTMNAMGHGIANPLGVDQRGVEQDIRALLPGYMAMGEKGMGEHQD
                     HTDSGHMQGPENTLPMMMGKGPFGNLEMGGMFTVLKVRDELAPGDFRDPGPYRNPPGT
                     VARRISNDPDFGRPVRRAAAR"
     gene            complement(670..933)
                     /locus_tag="PLW13_11440"
     CDS             complement(670..933)
                     /locus_tag="PLW13_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HQY71034.1"
                     /translation="MESPVHALGDLFRQLGLPDDAASIEQFIATHRPLAPHVPLAEAC
                     CWSASQAQFLREQIADDADWAEVVDSLNARLRGAPDGRNPGRC"
     gene            complement(1025..2395)
                     /locus_tag="PLW13_11445"
     CDS             complement(1025..2395)
                     /locus_tag="PLW13_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQY71035.1"
                     /translation="MSALLTEKDVFPWAERVLQGRITASTRQGGREAGGRPGWFVDVA
                     GGGTSAKYYIRGDRGGDFGYIQEYGLQREVQLLKLLRDEGIPVPEVIASSDEPNAAIL
                     GFVEGVNDFTRIASAAERDAVARDFARVMARWHAIPAGKFAEIGLAVPLNAEQLVGND
                     LSVWERGHFPLLSEPVPLVTFACQWLRRNLPPMPEKLVLVQGDTGPGQFLFRDGRVQA
                     VVDWELGALGDPMRELAHIRTRDVWYPTGNLPDWFRYYSEFSGTPLDDDRIRYYSVIG
                     MLTTALALAPVVQKLNPRDEHAEWLAQDVWSKCATAEAIAEAMGITLEDVALPAAARP
                     YVSALFDTLRENLEQEQLPHIEDSFRRHRLQMDLRLLARLRNQAEIGPEIEAMELADM
                     QALLGRRPRSLAEGQRSIDALVRAAGPGRDEEFVRYFHRHARREEALMRGGMGRAANA
                     RAAAIR"
     gene            complement(2467..4620)
                     /gene="glgP"
                     /locus_tag="PLW13_11450"
     CDS             complement(2467..4620)
                     /gene="glgP"
                     /locus_tag="PLW13_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQY71036.1"
                     /translation="MFPGERDIDRAVAELQEGLPEQLWPLARVAYNYRWCWAAEGPAV
                     FAAIDPERWARTGGNPLRLLTEAHRSTLGRAARDAGFVDRVGRLAHELAVDRARPWRA
                     GVASPDRPIAFCCAEFGVHGSLPIYSGGLGILAGDILKEASDLALPMVGVGLLYRTGY
                     FHQRIDVSGLQHEYWLDADPDRLPCVRVTDSDGRPLTVEVPINDESVTAQVWRVDVGR
                     VALYLLDTDLPVNSAVGRWVTSRLYESNRTIRLAQYAVLGVGGVRALRALGIAPAVYH
                     VNEGHGALGVFELLAQARAAGGVGYDAAWQRVREQVVFTTHTPVPAGNETYSREEILP
                     VLGRIADLAGEREQLLAAGRIDPGNAGQPSGMSALAVRASRRVNAVSRRHAEVARSMW
                     QPLFPGRALDEVPISHVTNGVHVPTWLRGPMRELLDRYLGEGWLGRADDPATWGRVDD
                     IPATELWAARCAARGQLVDMITQRSTAHRLSRGDPTDYAEAARNGFDPGRLTIGFARR
                     LATYKRLHLLALLPERAFAVLGGERPIQFIFAGKAHPADADAKEIVRHLFALKRAPSV
                     AGRAAFLEDYDMAIAGQLVAGCDVWVNLPRPPLEASGTSGMKSCLNGGLQLSVLDGWW
                     AEAWDGSNGWAINGDVDGDHQAQDYRDANTLFDLLQHEVIPLFHERDADGVPQRWVAM
                     MRRSLQTNGPRFCATRMVREYMQRIYPSGCLPSYQ"
     gene            complement(4751..5965)
                     /locus_tag="PLW13_11455"
     CDS             complement(4751..5965)
                     /locus_tag="PLW13_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71037.1"
                     /translation="MSKAQRSHVADLRGASRLAIDATAGLAGLVEAIHANVARTPTKL
                     GGPIVGGAVNGITGLVYNSVRGVTRAVGGGLDLALRAVPSALGEMESMPARERLVAVV
                     NGVLGDYLVATDNPLAITMRMRRDGKPLALSREALTSVIPSTSTKLLVLVHGLCMNDL
                     QWTRDGHNHGVALAKDLGYTSVYLHYNTGLHISTNGRAFAEALEALVAAWPVPLEELA
                     IVAHSMGGLVTRSALDMATGAGQAWPQLLRKLVFLGTPHHGAPLERGGHWIHTVLGKT
                     PYIAPLERLGRIRSAGITDLRHGSVHDEDWQGRDRFAHGNDPRRPLPLPRDVRCYAIA
                     GTIGSAETQLREQLLGDGLVPVPSALGQHRDARFTLAFPKSHQWIAADTSHFDLLGRA
                     AVYRRIRKWLAA"
     gene            6176..7288
                     /locus_tag="PLW13_11460"
     CDS             6176..7288
                     /locus_tag="PLW13_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71038.1"
                     /translation="MSHTLWIATRKGAFALRSDAARRNWTLSGPQFLGHIVHHIVQDP
                     RDPGVVLMAAKTGHLGPTMYRSTDRGRKWTEVKQPPAFRTAAHGEASRAVEAVFWLTP
                     GNREEPGAWYAGTIPAGLFRSEDGGDRWEPVAGFNDHPLRAQWAPGSGTPGGEILHSI
                     LVDPRDARHLYIGISIGGIFESTDGGQDWKPLNKGVEADFIPDPSIEFGHDPHCVALH
                     PLHPDRLYHQNHCGIYRIDRPAQEWKRIGRAMPRRIGDIGFPIVLHPRDPDTAWVFPM
                     DGTTVWPRTSIAGKPAVYVTRNGGRSWQRQDKGMPAAQAWFTVYRQAMCADTQQRVGV
                     YFGTTQGEVWASSNEGESWRSIARNLPQIHAVTHAV"
     gene            7308..7574
                     /locus_tag="PLW13_11465"
     CDS             7308..7574
                     /locus_tag="PLW13_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQY71039.1"
                     /translation="MSCTVRIASPLRSYTAGAAVVPATGDSVTEVLADLDQRFPGMRF
                     RMIDEQHRIRRHIRLFVNTTEVTALSQPVVDGDTVHLICALSGG"
     gene            7576..>8681
                     /gene="moeB"
                     /locus_tag="PLW13_11470"
     CDS             7576..>8681
                     /gene="moeB"
                     /locus_tag="PLW13_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HQY71040.1"
                     /translation="MSLSPAEVARYSRHLALREIGVAGQAKIRAAKVLVIGAGGLGSP
                     SVLYLAAAGVGTIGIVDFDVVDISNLQRQLLFDTASVGEPKADAAARRLRALNPGIDV
                     IAHRVELRASNVIGIVADYDVVLDGTDRFGTRYLVNDACVILGKPLVCAAIHRFEGQA
                     LTCIPGGGPCYRCLFPEPPAEGLVPNCAQAGVLGVLPGVLGTIQATEAIKIITGTGEL
                     LVGRLLTYDALEMRFDEFRFARREDCAVCGSDPAISAPQDAPQPCSVSSPEPVRCLSA
                     RQLQALLGDPSLVLVDVREPYEFEAGFLSGALNIPLAQLADRLLELPPLRTPVFVCRS
                     GTRSLAACALASQAGIQSPAHLEGGLLAWAQEVD"
BASE COUNT         1362 a         3047 c         2911 g         1361 t
ORIGIN      
        1 tcctgctgca caactgggcg ctgcacccgg gcacctggcg ccccgatccc tcgatcatga
       61 cggagttcga tctctggacc ttcaactcga aggtgtttcc cgcgatcgag ccgctggtcg
      121 cgcgcacggg cgagcgggtg cgcatccgcg tcggcaacct gtcgatgcac gagcacccgg
      181 tccacctgca cggcgtgcag ttccacgtca cgggcggcga cggcgggcgc ctgccgcaag
      241 cgctctggcg caccgaggtg acggagctcg tcggcgtcgg ccagatgcgc gatatcgagt
      301 tcgtcgcgat tgccggcgac tgggccctgc actgccacaa gtcgcaccac acgatgaacg
      361 ccatggggca cggcatcgcc aacccgctcg gcgtggacca gcgcggcgtg gaacaggaca
      421 tccgcgcgct gctgccgggc tacatggcga tgggtgaaaa gggcatgggc gagcaccagg
      481 accacaccga ctccgggcat atgcagggcc ccgagaacac cttgccgatg atgatgggca
      541 agggcccgtt cggaaacctg gagatgggcg gcatgttcac cgtgctgaag gtgcgcgacg
      601 agctcgcgcc gggcgacttc cgcgatcccg gcccctaccg caatccgccg ggcaccgtgg
      661 cgcggcgcat cagcaacgac ccggatttcg gccgtccggt gcgccgcgca gccgcgcgtt
      721 gaggctgtcg acgacttccg cccagtcggc gtcgtcggcg atctgttcgc gcaggaactg
      781 cgcctgcgag gcactccagc aacacgcttc cgccaatggg acatgcggcg ccagcggtcg
      841 gtgggtcgcg atgaattgct cgatgctcgc ggcgtcatcg ggcaggccca gctggcggaa
      901 caggtcgccc agggcatgaa caggactttc catggtcatt tcctcccgtg cgttggcgtg
      961 gcaacgatcc gcagcgcgca cgcgcagatc cgctcgtgac gatggccccg catccgcgct
     1021 cgcgctagcg gattgccgcg gcccgcgcat tcgccgcccg gcccatgccg cctcgcatca
     1081 gcgcctcttc gcgtcgcgca tggcgatgga agtagcgcac gaattcctcg tcgcgcccgg
     1141 gccccgcggc gcgcaccagc gcgtcgatgc tgcgctgccc ctcggccagg ctgcgcgggc
     1201 gcctgccgag cagcgcctgc atgtccgcaa gctccatcgc ctcgatctcg ggcccgatct
     1261 cggcctggtt gcgcagccgc gccagcaggc gcagatccat ttgcagccga tggcggcgaa
     1321 aggaatcctc gatgtgcggc agctgctcct gttcgagatt ctcccggagc gtgtcgaata
     1381 gcgccgacac atagggccgt gcggccgcgg gcagcgcgac gtcctcgagc gtgatgccca
     1441 tcgcctcggc aatcgcctcg gcggtggcgc acttggacca cacgtcctgc gcgagccact
     1501 cggcatgctc gtcgcgcgga ttcagcttct gcaccacggg cgcgagcgcc agtgccgtgg
     1561 tgagcatgcc gatcacgctg tagtaacgga tcctgtcgtc atcgagcggc gtgccgctga
     1621 actcgctgta gtagcggaac cagtccggca ggttgcccgt gggataccag acatcgcgcg
     1681 tgcggatgtg cgcgagttcg cgcatcgggt cgccgagcgc accgagctcc cagtccacca
     1741 cggcctgcac gcgcccgtca cggaacagga actgcccggg gccggtgtcg ccctgcacca
     1801 gcaccagctt ctccggcatc ggcggcagat tgcgcctgag ccactggcag gcgaaggtca
     1861 ccagcggcac cggctcgctg agcagcggaa aatgcccgcg ctcccagacc gacaggtcat
     1921 tgcccaccag ctgctcggcg ttgagcggca cggccagccc gatctcggcg aacttgccgg
     1981 ccgggatcgc gtgccaccgg gccatcacgc gcgcgaagtc gcgcgccacg gcgtcgcgct
     2041 cggcggcgct tgcgatccgg gtgaaatcgt tgacgccctc gacgaagccg aggatcgccg
     2101 cgttcggctc gtcgctgctc gcaatcacct cgggcaccgg gatgccttca tcgcgcagca
     2161 gcttcagcag ctgcacctca cgctgcagcc cgtactcctg gatgtaaccg aagtcgccgc
     2221 cgcgatcgcc gcggatgtag tacttcgcgc tcgtgccgcc gccagcgacg tcgacgaacc
     2281 agcccggacg gccgccggcc tcgcgcccgc cctggcgggt actcgcggtg atgcgcccct
     2341 gcagcacgcg ctcggcccag gggaatacgt ccttctcggt gagcagtgcg gacacggaac
     2401 ggcctccagg gaattcctcc gctctggcaa ccagcggcgc cggagcggcg gggaatcatc
     2461 gcgcgatcat tgatacgaag gcaaacagcc gctcgggtat atccgctgca tatactcgcg
     2521 gaccatgcgc gtggcgcaga agcgcggccc gttcgtctgc aggctgcggc gcatcatcgc
     2581 cacccagcgc tgcggcacgc cgtcggcgtc gcgttcgtgg aacagcggga tcacctcgtg
     2641 ctgcagcagg tcgaacaacg tgttggcgtc gcggtagtcc tgcgcctggt gatcgccgtc
     2701 cacgtcgcca ttgatcgccc agccgttgct cccgtcccag gcctccgccc accagccgtc
     2761 gagcaccgac agctgcaggc caccgttgag gcacgacttc atgccgctgg tcccgctggc
     2821 ctcgagcggc gggcgcggca ggtttaccca cacgtcgcag ccggcgacga gttgcccggc
     2881 gatggccatg tcgtagtcct ccaggaacgc agcccgcccg gcaacgctcg gcgcgcgctt
     2941 gagcgcgaac aggtggcgca cgatctcctt ggcgtcggcg tcggcgggat gggccttgcc
     3001 ggcgaagatg aactggatcg ggcgttcgcc gccaaggacg gcgaatgccc gttccggcaa
     3061 cagcgcaagg aggtgcaggc gcttgtaggt cgcgagacgg cgggcgaagc cgatggtgag
     3121 gcgcccggga tcgaacccgt tgcgcgcggc ctcggcgtag tccgtcgggt cgccacgact
     3181 cagtcgatgg gccgtgcttc gctgcgtgat catatcgacc agctgcccgc gtgccgcgca
     3241 gcgcgccgcc cagagctcgg tcgcggggat gtcgtccacc cgcccccagg ttgccggatc
     3301 atcggcgcga cccagccagc cttcacccag gtatcggtcc agcagttcgc gcatcggacc
     3361 acgcagccag gtgggcacgt gcacgccgtt cgtgacatgg ctgatcggga cctcgtccag
     3421 cgcacggccc gggaacagcg gctgccacat cgagcgggcc acctcggcgt ggcgcctgct
     3481 gaccgcgttg acccgacggc tcgcgcgcac ggcgagcgcc gacatgcccg aaggctggcc
     3541 ggcattgccc gggtcgatgc ggccggcggc gagcagttgc tcacgctcgc cggccaggtc
     3601 ggcgatacgc cctagcaccg gcagtatctc ttcgcgcgaa taggtctcgt tgccggccgg
     3661 caccggggtg tgggtcgtga aaaccacttg ctcgcgcacc cgctgccacg ccgcgtcgta
     3721 gccgacaccg ccagcagccc gggcctgggc cagcagctcg aatacgccga gcgcgccgtg
     3781 cccctcgttg acgtgataga cggccggagc gatgccgagc gcgcgcaggg cccgcacccc
     3841 gccgacgccg agcacggcgt actgcgccag ccggatcgtg cggttgcttt catagagccg
     3901 tgacgtgacc cagcgtccga ccgcgctgtt gaccgggaga tcggtgtcga gcaggtagag
     3961 cgccactcgc cccacgtcga cgcgccacac ctgcgcggtg acgctttcat cgttgatggg
     4021 aacctcgacg gtgagcggcc gcccgtcgct atcggtgacc ctgacgcacg gcagccggtc
     4081 gggatcggcg tcgagccagt attcgtgctg caggcccgag acgtcgatgc gctggtggaa
     4141 ataacccgtg cgatacagca gcccgacccc gaccatcggc agcgccaggt cggaggcttc
     4201 cttcaggata tcgccggcca gtatgccgag cccgcccgag tatatgggca gcgaaccatg
     4261 cacaccgaat tcggcacagc agaacgcgat cgggcgatcc gggctggcca caccggctcg
     4321 ccagggcctc gcccggtcaa ctgcgagttc gtgcgccaga cgcccgaccc ggtcgacgaa
     4381 tccggcgtcg cgggcggcgc gcccgagcgt cgagcggtgc gcctcggtga gcaggcgcaa
     4441 cggattgccc ccggtgcgcg cccagcgctc gggatcgatc gccgcaaaga ccgcgggacc
     4501 ctcggcggcc cagcaccacc ggtagttgta ggccacgcgc gcgagcggcc acagctgctc
     4561 gggaaggccc tcctgcaatt cggcgacggc gcgatcgatg tcgcgctcac cggggaacat
     4621 gattcactgc catctgaagg gtttccggat accaggtgcg cgaggctcac tatctttgtg
     4681 cgcccgcgct cctgtcaatc cggtccgccg gacgcatcct gtcgatgtgc acccgggctg
     4741 acgcacctcg tcatgccgcg agccacttgc ggatccggcg atacaccgcg gcccggccga
     4801 gcagatcgaa gtggctggtg tccgcggcga tccactggtg cgacttcgga aacgccagcg
     4861 tgaagcgcgc gtctcgatgc tgcccgagcg cgctcggcac cggtacgagg ccgtccccga
     4921 gcagctgctc acgcaactgc gtttccgcgc tgccgatcgt tcccgcgatc gcgtagcagc
     4981 gcacatcgcg cggcagcggc aaggggcgcc gcggatcgtt gccgtgcgcg aagcgatcgc
     5041 ggccctgcca gtcctcgtca tgcacgctgc cgtgacgcag atcggtaatg ccggcgctgc
     5101 gaatccgtcc gaggcgctcc agcggcgcga tgtagggcgt cttcccgagc acggtgtgga
     5161 tccagtgccc gccccgctcg agcggcgcgc catgatgcgg cgtaccgaga aaaacgagct
     5221 tgcgcagcaa ttgcggccac gcctgaccgg cgcccgtggc catatcgagc gcgctgcgcg
     5281 tgaccagccc acccatgctg tgcgccacga tggcgagttc ctcgagcggc accggccagg
     5341 cggcgacgag agcttcgagt gcttcggcga acgcacgtcc gttggtcgaa atgtgcagcc
     5401 cggtgttgta gtgcagatag acggacgtgt agcccaggtc cttcgcgagc gccacaccgt
     5461 ggttgtgccc gtcgcgcgtc cactgcagat cgttcatgca cagaccgtgg acgaggacca
     5521 gcagcttcgt gctcgtgctc ggtatcaccg aggtgagcgc ctcgcggctc agcgcgagcg
     5581 gcttgccgtc acggcgcatg cgcatcgtga tcgccagcgg gttgtccgtc gcgaccaggt
     5641 agtcgccgag cacaccgttc accacggcca ccaggcgttc gcgcgccggc atcgactcca
     5701 tttctccgag cgcggatggc accgcgcgca gtgcgagatc gaggccgccc cccaccgcgc
     5761 gcgtgacgcc cctgacgctg ttgtagacca gtccggtgat cccgttcacc gcgccaccga
     5821 cgatggggcc acccagcttc gtcggcgtgc gcgcgacgtt ggcgtgaatc gcctcgacca
     5881 gcccggcgag ccccgccgtg gcatcgatag ccaggcggct ggcgccgcga agatcagcaa
     5941 cgtgactgcg ttgcgccttg ctcatggatg atcccctgat tgcatgaacc ggataccccg
     6001 gcgtcccggc gaatcgactc gcggcccggc accgccccga agaatcctcc cgaatcctgg
     6061 agcaatcaac ccaactcgtc gcgacatatg gcaggcgatg ctgtccgcgt gcgagttgac
     6121 aggcatcggc ttcggcgcga tcgtgacatc ccgcctggat ctccgggagt gcgttatgag
     6181 ccacacactc tggatcgcca cgcgcaaggg cgcgttcgcg ctgcgcagcg atgccgcccg
     6241 gcgcaactgg acattgagcg gcccgcagtt cctggggcac atcgtccacc acatcgtgca
     6301 ggacccgcgt gatcccgggg tggtcctgat ggccgcgaaa acgggacacc tcggtccgac
     6361 gatgtatcgc tcgacggatc gcgggcgcaa gtggacggaa gtgaagcagc cgccggcgtt
     6421 tcgcaccgca gctcacggcg aggcgagccg cgccgtggag gcggtgttct ggctgacgcc
     6481 cggaaaccgg gaggagccgg gcgcgtggta cgcgggcacg attccggcgg gcctgttccg
     6541 ctcggaagac ggtggcgatc gctgggaacc ggtggcaggc ttcaacgatc atccgctgcg
     6601 cgcgcaatgg gcgccgggct cgggcacgcc gggcggcgag atcctgcact ccatcctcgt
     6661 cgatccgcgc gatgcgcggc acctgtatat cggcatatcg attggcggga tcttcgagtc
     6721 caccgacggc ggccaggact ggaagccgct gaacaagggc gtggaagcgg atttcattcc
     6781 ggatccctcc atcgagttcg gtcacgaccc gcactgcgtg gcgctgcatc ccctgcatcc
     6841 cgatcgcctg tatcaccaga accactgcgg catctaccgc atcgatcgtc ctgcacagga
     6901 atggaagcga atcggcaggg ccatgcccag gcgcatcggc gacatcggct tcccgatcgt
     6961 gctgcatccg cgcgacccgg ataccgcgtg ggtgttcccc atggacggca ccaccgtctg
     7021 gccgcgaacc tccatcgccg ggaagccggc ggtgtacgtg acgcgcaacg gcggcaggag
     7081 ctggcaacgc caggacaagg gcatgcccgc cgcgcaggcg tggttcacag tgtatcgcca
     7141 ggccatgtgc gccgatacgc agcaacgcgt cggcgtttac ttcggcacga cgcaagggga
     7201 ggtatgggcg agcagcaatg agggcgaatc atggcggagc atcgcgcgca acctgccgca
     7261 gattcatgcg gtcacgcacg cggtataggc caccccggct cccccccatg tcctgcaccg
     7321 tcagaatcgc cagccccctg cgctcctaca ccgcaggcgc cgctgtagtg cctgccaccg
     7381 gcgattcggt gacagaggtg ctcgccgacc tggatcagcg cttccccggc atgcgctttc
     7441 gcatgatcga cgagcagcat cgcatccgcc gccatatccg cctgttcgtg aacaccaccg
     7501 aggtgacagc gctgtcgcaa cccgtggtcg acggcgacac ggtgcacctg atctgcgccc
     7561 tgagcggagg ctgaggtgag cctgtcgccg gccgaagtgg cccgctacag ccgtcacctc
     7621 gcgctgcgcg agatcggggt cgcgggacag gcaaaaatca gggccgcgaa ggtgctcgtc
     7681 atcggcgccg gcggcctggg ctcgccatcg gtcctctacc tcgccgcggc gggcgtgggt
     7741 acgatcggca tcgtcgattt cgacgtggtc gatatctcca atctccagcg ccagctgctg
     7801 ttcgacaccg ccagcgtggg cgaacccaag gccgatgcag cggcacggcg cctgcgtgcg
     7861 ctgaatcccg ggatcgacgt gatcgcgcat cgcgtcgagc tgcgtgcgag caacgtgatc
     7921 gggattgtcg cggattacga tgtcgtgctc gacggcacgg atcgcttcgg cacgcgttac
     7981 ctcgtcaacg atgcctgcgt gatcctcggc aaacccctgg tgtgcgcggc cattcatcgc
     8041 ttcgaaggcc aggcactgac ctgtattccc ggtggcggtc cgtgctatcg ctgcctcttc
     8101 cccgagccgc ctgccgaggg gctggtgccc aattgcgcgc aggccggcgt gctcggtgta
     8161 ttgcccgggg tgctcggcac gatccaggcg acggaagcga tcaagatcat cacgggcacc
     8221 ggcgagctgc tggtcgggcg cttgctcacg tatgacgcgc tcgagatgcg cttcgatgaa
     8281 ttccggttcg cgcgccgcga ggactgtgcc gtgtgcggct ccgatcccgc catttctgcg
     8341 ccgcaggatg cgccgcaacc gtgcagcgtc tcatcgcctg aaccggttcg ctgcctgtcg
     8401 gcacgccagc tgcaagcgct gctcggcgat ccctcgctgg tgctcgtgga tgtgcgcgag
     8461 ccgtatgaat tcgaggccgg tttcctgagc ggtgcgctga acattcccct ggcgcaactt
     8521 gccgatcgtc tgctcgagct gccgccattg cgcacgccgg tctttgtctg ccgcagtggc
     8581 acccgcagcc tcgccgcatg tgcgctcgcc tcgcaagcgg gcatccagtc cccggcgcac
     8641 ctcgaaggcg gcctgctggc gtgggcgcag gaggtggatg a
//
LOCUS       DAORUQ010000133         8641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_8955_length_8641_cov_5.284300, whole genome shotgun sequence.
ACCESSION   DAORUQ010000133 DAORUQ010000000
VERSION     DAORUQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8955_length_8641_cov_5.284300"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLW13_11475"
     CDS             complement(<1..371)
                     /locus_tag="PLW13_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71041.1"
                     /translation="MRERNELILADLIALRAEQKPELDVLTFEHYSLDDGATADEVRT
                     YAQLFTNANRLAAWLLARGMQPGDRFVITLRNHPEFVEAMVAASMTGTVFVPVDPRTR
                     ADKLAFMVNNSGSRGIVCANY"
     gene            complement(392..1603)
                     /locus_tag="PLW13_11480"
     CDS             complement(392..1603)
                     /locus_tag="PLW13_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY71042.1"
                     /translation="MRVNAIVAGVGMTPFGKHMDKSIKWLGGQPALEAIQDAGISPTE
                     IEAAYVGNCAAGLVTGQESIRGQVVLSSIGLGKFPIINIENACGSGSTALNQAAMMIS
                     AGYYDVVLVVGFEKLFHENKAISYSAFGGAIDVELRDRFMAANTAGQSEGSGTKRSMF
                     ADFYGVLAREFMASHGTELRHFAMVSAKNSVHGSLNPRAQFRKAVTVEEVLAEPVIVD
                     PLTRPMICPLADGGAAVVLVSERKARALGIRKPVRIVASVVNSYFEHPDGATENVSSI
                     AIADAYHEAGVGPGDLSLVELHDSSAVTELITYESLGLCAPEDIAGALERGETSLGGR
                     IPVNTSGGLLRKGHPVGATGVAQLAEIAWQLQGRAGARQVEGARVGLCHNGGGTLGND
                     TAVMNITIAMR"
     gene            complement(1626..2414)
                     /locus_tag="PLW13_11485"
     CDS             complement(1626..2414)
                     /locus_tag="PLW13_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY71043.1"
                     /translation="MELREAIGTRRSYRYLDPDRPVELEKIQRMLEAARIASHFGNNN
                     AARALVVDRHSATQEQIDSLPSPVGGFQIQQAPIVILWYVEGTAMDYAADRLIELVDI
                     GALGYGPDRYEELNNTLVPVFRKSADALKGPGMVDMDLGQAVAQATLMAIEQGLGCCC
                     QGSANWKKVEKAFNFGEQQRVVVVQTVGYPAESKEAGGQRPRQPFEQLFQLNSLANPF
                     PRSQAVVEELTRDRFFQAPAKLPGREEELEALRQKYNLPRAGMI"
     gene            complement(2459..3541)
                     /locus_tag="PLW13_11490"
     CDS             complement(2459..3541)
                     /locus_tag="PLW13_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY71044.1"
                     /translation="MIAGDSPLWGVLEGFKALGFAGGASVDDPSLAPAEKALLHCIVL
                     EELSAGDVGLFITCGLLNIAPEIARASGRADLTEFFGARDELCCLALTEPGHGSDIVA
                     FTEPAYRNPRIKPALKCRRDGANYVLNGQKAAWVSGGTLAGSGIVFASLENSPVGLAD
                     GAVFLMPFDLPGVSKGKPLEKIGQRALNQGEIFFDNVEVPAQFLLGEGPELYPLIWEM
                     NLKGANIAMGQQFVGVARAAYDLALDYARQWVQGGCPIIEHQNVKTRIFGMFQKLEVA
                     RSHVRRVAIADATLPGGVPFQYAASAKVFATQTAFEVAHDAIQVLGGNGLTHEYLAEK
                     LFRDARSSLIEDGENGMLGLMAASRL"
     gene            complement(3736..4434)
                     /locus_tag="PLW13_11495"
     CDS             complement(3736..4434)
                     /locus_tag="PLW13_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71045.1"
                     /translation="MKKATTGSGAVPPRRRGRPEGSGVATVTREKILRAAVYCFARSG
                     YAQTSNRDIAQAAGITSGSLYHYFDSKSAIFQEALRQCTLALVETYRAASVEAAGRSC
                     VDQLCLGLERVILLSRDWPGIIRFGGNAAAEIRHNRELEWLRADVADAFPAFFRELMQ
                     RAEARGELAEGVGVDDAASLLLTLTMGLSISHETDGDEDQFAARVRAYERLLRGQLLR
                     TPHDPAQSGQRQPQ"
     gene            complement(4431..5423)
                     /locus_tag="PLW13_11500"
     CDS             complement(4431..5423)
                     /locus_tag="PLW13_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY71046.1"
                     /translation="MTFSTALRLNMTGLQPDAREEGKRYRAALDMASYVDAHGFDAVA
                     LEEHHGAGNGWLPSPLTMAAAVAARTRRVRISVTALLVTLYDPVRLAEDIAVLDLISD
                     GRFSFVAGSGYRPEEYHAVGKDWDARGRLMDEVIDTLLEAWRGEPFDYKGRRIRVTPV
                     PQSRPHPLFFVGGMSAVAARRAARFGLPFYPPFEDPALEKVYREALEHHGKRGFVVHP
                     GAGNTMLFVDPDPGQAWERLGPYFLREMQEYDSWKVEGVRRPSEGAAASVDELRRSGR
                     FAIMTPAEARALLGDGGKHNAVLHPLAGGVPLDAAWASLRLFVEQVLQPARNRE"
     gene            5653..6861
                     /gene="pcaF"
                     /locus_tag="PLW13_11505"
     CDS             5653..6861
                     /gene="pcaF"
                     /locus_tag="PLW13_11505"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HQY71047.1"
                     /translation="MARDAFICDAVRTPIGRYGGVLAGVRTDDLGAVPLRALMARNPG
                     VDWSGVDDVYYGCANQAGEDNRNVARISALLAGLPLTVPGVTLNRLCGSGMDAIGSAA
                     RAIRCAEASLVLAGGVESMSRAPFVMPKADSAFSREAQIYDTTLGWRFVNPLIELQYG
                     VDSMPETGENVAAGFGIARADQDLFAWRSQQRAGAAITSGFFAREIVPVSIARRRQEP
                     LLIDRDEHPRPDTTLEALARLPTPFRDNGTITAGNASGVNDGACALLLASGDAAKQHG
                     LTPRARVVAMAVAGVEPRIMGMGPVPATRKVLALAGLQLGQMDVIELNEAFAAQALAV
                     LRELGLPDDAAHVNPNGGAIALGHPLGMSGARLVTTALCELERRGGRYALCTMCIGAG
                     QGIAMIIERV"
     gene            7136..8404
                     /locus_tag="PLW13_11510"
     CDS             7136..8404
                     /locus_tag="PLW13_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY71048.1"
                     /translation="MRDETMLIHAGYAGDPTTHSVATPIYQTVAYEFDSAQHGADLFN
                     LAVPGNIYTRIMNPTTDVLEKRVAALEGGVGALALSAGSAAINYAVLNIATVGRNIVS
                     TPQLYGGTYTLFAHMLPAQGIKVKFAKSDRPEDIAAEIDADTRAIFCESIGNPAGNIA
                     DLAGLAAVANAHGVPLIVDNTVATPMLTKPFAFGAHIVVHSLTKYMGGHGNSLGGIIV
                     DSGTFPWAQHAAKFPMMTTPEPSYHGVVYTEAMGPAAYIGRARTVPLRNTGSALSPFN
                     AFLLLQGIETLALRMERHCSNALAVANYLQSHPRVSIVSYAGLPGHPYHALAQKYCTG
                     GLPASLLTFEVKGGMDEAVRFYDALQLFKRLVNIGDAKSLACHPASTTHRQMTPEEQA
                     RAGVTPGMIRLCVGIEHIDDIVADLAQALG"
BASE COUNT         1317 a         2952 c         2972 g         1400 t
ORIGIN      
        1 ttgtagttcg cgcacacgat gccgcggctg ccggagttgt tcaccatgaa cgcgagcttg
       61 tcggcgcgcg tgcgcgggtc caccggcacg aacacggtgc cggtcatcga ggcggcgacc
      121 atcgcctcga cgaactccgg gtggttgcgc agggtgatca cgaagcggtc gcccggctgc
      181 atgccgcgcg cgagcagcca ggcggcgagg cggttggcgt tggtgaacag ctgcgcgtac
      241 gtgcgcacct cgtcggccgt ggcgccgtca tcgaggctgt agtgctcgaa agtcagcacg
      301 tcgagctcgg gtttctgttc cgcgcgcagc gcgatcaggt cggccaggat cagttcgtta
      361 cgctcgcgca cggtggtggc tctcccttcg atcagcgcat ggcgatcgtg atgttcatca
      421 ccgcggtgtc gttgccgagc gtgccgccgc cgttgtggca caggccgacg cgcgcgccct
      481 cgacctggcg cgcgccggcg cgcccctgca gctgccaggc gatctccgcg agctgcgcga
      541 cacccgtggc gcccacgggg tggcccttgc gcagcagtcc gcccgaggtg ttcaccggta
      601 tacgcccgcc gagcgaggtt tcgccgcgct ccagcgcacc cgcaatgtcc tccggtgcgc
      661 acaggccgag gctttcgtag gtgatcagtt cggtaacggc ggaggagtcg tgcagctcga
      721 ccagactcag gtccccgggg cccacgccgg cctcgtgata ggcgtcggcg atcgcgatgc
      781 tgctgacgtt ctcggtcgcg ccgtcggggt gctcgaagta ggagttcacc accgaggcca
      841 cgatgcgcac gggcttgcgg atgccgagtg cccgcgcctt gcgctcgctg accagtacca
      901 cggccgcgcc accatcggcg agggggcaga tcatcgggcg cgtcagcgga tcgacgatta
      961 ccggctctgc cagtacctcc tcgaccgtca cggccttgcg gaactgcgcg cgcggattga
     1021 ggctgccgtg cacggagttc ttcgccgaga ccatcgcgaa atggcgcagc tccgtgccgt
     1081 gcgaggccat gaattcgcgt gccagcacgc cgtagaagtc ggcgaacatc gagcgcttcg
     1141 tgcccgagcc ctcgctctgg cccgcggtgt tggcagccat gaaccggtcg cgcagctcga
     1201 cgtcgatcgc gccaccgaag gcgctgtatg aaatggcctt gttctcgtgg aacagcttct
     1261 cgaagcccac caccagcacc acgtcgtaat agcccgcgga gatcatcatc gccgcctggt
     1321 tcagcgccgt cgagcccgag ccgcaggcat tctcgatatt gatgatcggg aacttgccga
     1381 gaccgatcga gctcagcacc acctggccgc ggatcgattc ctgcccggtc acgaggccgg
     1441 ccgcgcagtt gcccacgtag gcggcctcga tctccgtcgg gctgatgccg gcatcctgta
     1501 tcgcctcgag cgccggctgg ccgccgagcc acttgatgct cttgtccatg tgcttgccga
     1561 acggggtcat gcccacaccg gcgacgatcg cattcactcg catggcagca gccctcccgg
     1621 ggtggtcaga tcatgccggc gcgcggcagg ttgtacttct ggcgcagggc ctcgagctct
     1681 tcctcgcgac ccggcagctt ggccggtgcc tggaagaagc ggtcgcgcgt cagctcctcg
     1741 accaccgcct gcgagcgcgg gaaggggttg gcgagcgagt tcaactggaa cagctgctcg
     1801 aagggttggc gcggacgctg gccgccggcc tccttcgact cggccgggta gcccacggtc
     1861 tgcaccacca cgacgcgctg ctgctcgccg aaattgaagg ccttctcgac cttcttccag
     1921 ttcgccgagc cctggcaaca gcagccgagg ccctgctcga tggccatcag cgtggcctgc
     1981 gcaacggcct ggccgaggtc catgtcgacc atcccgggcc ccttcagcgc gtcggctgat
     2041 ttgcgaaaga ccggtaccag cgtgttgttc agttcctcgt agcggtcggg cccgtagccg
     2101 agcgcgccga tgtcgacgag ctcgatcagg cgatcggccg cgtagtccat cgcggtgcct
     2161 tccacgtacc acaggatcac gatcggcgcc tgctggatct ggaagccgcc gaccggcgag
     2221 ggcagggagt cgatctgctc ctgcgtggcc gaatggcggt ccaccaccag cgcgcgcgcg
     2281 gcgttgttgt tgccaaagtg cgaggcgatg cgcgcggcct ccagcatgcg ctggatcttt
     2341 tccagctcca ccgggcggtc cgggtcgagg tagcggtagg agcggcgggt gccgatggct
     2401 tcgcgaagtt ccatgtgact gtcctttggg agtgcggttg ggtgagttga agggttggct
     2461 agaggcgcga ggcggccatc aggccgagca tgccgttctc gccgtcctcg atcagcgagg
     2521 agcgcgcgtc gcggaacaac ttctcggcca ggtactcgtg cgtgagcccg ttgccgccga
     2581 gaacctggat cgcgtcgtgc gcgacttcga aggcggtttg cgttgcgaac accttggccg
     2641 acgccgcgta ctggaagggt acaccgccgg gcagcgtcgc gtcggcgatc gccacgcggc
     2701 gcacgtgcga gcgggcgacc tcgagcttct ggaacatgcc gaagatgcgg gtcttcacgt
     2761 tctggtgctc gatgatcggg cagccaccct gcacccactg cctggcgtag tccagcgcca
     2821 ggtcgtaggc cgcgcgcgcc acgccgacga actgctggcc catcgcgatg ttggcgccct
     2881 tgaggttcat ttcccagatc agagggtata gctcgggtcc ctcgccgagc aggaactgcg
     2941 ccggcacctc gacgttgtcg aagaagatct cgccctggtt cagcgcgcgc tggccgatct
     3001 tctccagcgg cttgcccttg ctcacaccgg gcaggtcgaa aggcatcagg aacaccgccc
     3061 cgtcggcgag ccccaccggg gaattctcta agctggcgaa cacgatgccc gagcccgcca
     3121 gcgtgccgcc cgagacccag gcggccttct gcccgttcag cacgtagttc gccccgtcgc
     3181 ggcggcattt cagcgcgggc ttgatgcgcg ggttgcggta ggcgggctcg gtgaaggcca
     3241 cgatgtcgct gccgtgcccg ggctcggtga gcgcgagaca gcacagctcg tcgcgcgcgc
     3301 cgaagaactc ggtcaggtcc gcacgcccgg aggcgcgggc gatctccggg gcgatgttca
     3361 gcaggccgca ggtgatgaaa aggcccacgt caccggcgct cagttcctcc agcacgatgc
     3421 agtgcagcag cgccttctct gccggtgcga gcgaagggtc gtcgaccgag gcgccgccgg
     3481 cgaagccgag cgccttgaag ccctcgagca cgccccacag cggcgagtcc cctgcgatca
     3541 cggcgtcggc tgacatccgg tccagcgcta tgccggcggg acgcatgact tcggtcgcga
     3601 atcggcggca ggtgtcgcgg attgcccgca tttcatccga aagtccagga tcgagatcga
     3661 aatccatggg gcaccctcgc cagtgtcgct cgtcatgtca gtattggatc agccaataat
     3721 caactcgtca atagattatt gtggctggcg ctgcccactc tgggccgggt cgtgcggcgt
     3781 gcgcagcagt tggcctcgca gcaggcgctc gtaggcgcgc acgcgcgccg cgaactggtc
     3841 ctcgtcgccg tcggtttcat gcgaaatcga gagccccatg gtcagcgtca acagcaggct
     3901 ggcggcgtcg tcgacgccga cgccctcggc cagttcgccg cgcgcctcgg cgcgttgcat
     3961 caactcacgg aagaaggctg ggaaagcgtc ggcgacatcc gcgcgcagcc attccagctc
     4021 gcggttgtgg cgtatctcgg cggcggcgtt gccaccgaat cggatgattc ccggccagtc
     4081 gcgcgacagc aagatcacgc gctccagccc caggcacagc tggtcgacgc agctgcgccc
     4141 ggcggcctcg acgctggcgg cacggtaggt ctcgaccagc gcgagggtgc actggcgcag
     4201 cgcctcctgg aagatcgcgg acttggagtc aaagtagtgg tacagcgagc cgctggtgat
     4261 gcccgcggcc tgtgcgatgt cgcggttgct ggtctgcgca taacccgagc gcgcgaaaca
     4321 gtagaccgcg gcacgcagga ttttctcgcg cgtgaccgtg gccacgccgc tgccctcggg
     4381 acgtccgcgg cggcgcggtg gcacggcgcc ggatcccgtg gtggctttct tcattcgcgg
     4441 ttgcgcgccg gctgaagcac ctgctcgacg aacagccgca acgatgccca ggccgcatcg
     4501 agcggcacgc cgccggccag cgggtgcaat accgcgttgt gcttgccacc gtcgccgagc
     4561 agcgcgcgcg cctcggcagg cgtcatgatc gcgaagcgac cgcttcgcct gagctcatcg
     4621 acgcttgccg cggcgccttc gctggggcgt cgcacgccct cgactttcca gctgtcgtac
     4681 tcctgcatct cgcgcaggaa ataggggccg aggcgttccc aggcctgccc gggatccggg
     4741 tccacgaaca gcatcgtgtt gcccgcgccc gggtgaacga cgaagccgcg cttgccgtgg
     4801 tgctcgaggg cctcgcgata gaccttctcc agcgcgggat cctcgaacgg cggatagaag
     4861 ggcagcccga agcgcgcggc gcggcgtgcg gccacggcgc tcatgccccc gacaaagaac
     4921 agcgggtgcg gacgcgattg cggcacgggc gtcacgcgga tgcgccgccc cttgtagtcg
     4981 aacggttcgc cgcgccacgc ctcgagcagg gtgtcgatca cctcgtccat cagtctcccg
     5041 cgcgcgtccc agtccttgcc gacggcatgg tattcctcgg gccggtagcc gctgccggcc
     5101 acgaagctga agcgtccgtc gctgatcagg tccagaacgg cgatgtcctc cgccaggcgc
     5161 acggggtcgt agagcgtcac cagcagggcg gtcacgctga tgcgcacgcg ccgcgtgcgc
     5221 gccgcgacgg cggccgccat ggtcagcggg ctgggcagcc agccgttccc ggcgccgtga
     5281 tgctcttcca gcgccacggc atcgaagccg tgcgcatcga cgtaggacgc catgtccagt
     5341 gccgcccggt agcgtttgcc ctcttcacgg gcgtcgggct gcaggcccgt catgttgagg
     5401 cgcagggcgg tgctgaacgt catgggttgc gcaattgcat ggcttgcccc tggcgcggcg
     5461 tgatgatggg agaatcgcgg gctggagcat atccccggct ctcgccgcgg cgcaaacgcg
     5521 gtgtttccgc gcgccggttg cctgcgctga tcgaggcccc ggcgcgagcg cgccccgggc
     5581 aattgacgag cgtgcgctgc gctgtggcat gatcctgctc ccgccggcga gcagtgacga
     5641 gggaggcgaa tcatggccag ggacgcattc atctgtgacg ccgtgcgcac gccgatcggg
     5701 cgctacggcg gtgtgctggc cggcgtgcgc accgacgacc tcggtgccgt gccgctgcgg
     5761 gcgctgatgg cgcgcaaccc gggcgtcgac tggtctggcg tggacgacgt gtactacggt
     5821 tgcgcgaacc aggcgggcga ggacaaccgt aacgtggcgc gcatcagcgc gctgctggca
     5881 ggcttgcccc tgaccgtgcc cggtgtgacg ctcaaccgcc tgtgcggcag cggcatggac
     5941 gcaattggtt cggcggcgcg cgcgatccgc tgcgccgagg cctcgctggt gctcgccggc
     6001 ggtgtcgagt cgatgtcgcg tgcgcccttc gtgatgccca aggccgacag cgccttcagc
     6061 cgcgaggcgc agatatacga caccacgctc ggctggcgct tcgtgaatcc gctgatcgag
     6121 ttgcagtacg gcgtggactc gatgccggag accggcgaaa acgtcgccgc ggggttcggc
     6181 atcgcgcgcg ccgaccagga cctgttcgcg tggcgcagcc agcagcgcgc gggcgcggcc
     6241 atcacctccg gctttttcgc ccgcgagatc gtgccggtca gcatcgcgcg gcgccggcag
     6301 gagccgctgc tgatcgatcg cgacgagcat ccgcgcccgg acaccacgct ggaggcgctg
     6361 gcgaggctgc ccacgccgtt tcgcgacaac ggcacgatca cggcgggcaa tgcctcgggc
     6421 gtgaatgacg gcgcctgcgc gttgctgctg gcctcgggcg atgccgcgaa gcagcacggg
     6481 ttgacgccgc gcgcgcgggt ggtggcgatg gccgtcgcgg gcgtggagcc gcgcatcatg
     6541 ggcatggggc cggtgccggc cacccgcaag gtgctcgcgc tcgcggggct gcaactcggg
     6601 cagatggacg tgatcgagct gaacgaggcc ttcgcggcgc aggccctcgc ggtgctgcgc
     6661 gagctggggc tgccggatga cgcggcgcac gtgaacccca acggtggtgc catcgcgctc
     6721 ggtcatccgc tcggcatgag cggtgcgcgc ctggtgacca ccgcgctctg cgagctggaa
     6781 cgccgtggcg ggcgctatgc gctctgcacc atgtgcatag gcgcggggca gggcatcgcg
     6841 atgatcatcg agcgggtgtg agcgcaagca gacgggcgaa gcatcgcgtt ttcgttgcgg
     6901 ccaacttgct gcgggctggc gaggccggag atccagccgc gacacgccgt aaatacatcc
     6961 ctgtaggctc gactgcggca tccatgccgc agacggtcgc cgctggagtt ccggccttgc
     7021 cagcgtgcgc gttcggtggc tacggtccgc agcaatggtg cttggcacaa ccgctttgtg
     7081 ctccttaaga tggcggtctg cgaatcaact ccacaacaag accaggtgcc agcaaatgcg
     7141 cgatgaaacc atgctgatcc acgccggcta cgccggtgac ccgaccacgc actcggtcgc
     7201 cacaccgatc taccagacgg tcgcctacga gttcgacagc gcgcagcacg gcgcggacct
     7261 gttcaacctc gcggtgccgg gcaacatcta cacgcgcatc atgaacccga ccaccgacgt
     7321 gctcgagaag cgcgtcgccg cgctcgaggg cggcgtgggc gcgctcgcgc tgagcgccgg
     7381 cagtgccgcg atcaactacg cggtcctcaa catcgcgacc gtcgggcgca acatcgtctc
     7441 gacgccgcag ctctacggcg gcacctacac gctgttcgcg cacatgctgc ccgcgcaggg
     7501 catcaaggtg aagttcgcca agagcgacag gcccgaggac atcgccgccg agatcgatgc
     7561 cgacacgcgc gcgatcttct gcgagagcat cggcaacccg gccggcaaca tcgccgacct
     7621 ggcgggcctc gcggcggtag ccaacgcgca cggtgtgccg ctgatcgtcg acaacaccgt
     7681 ggccacgccg atgctgacca agccctttgc cttcggcgcg cacatcgtgg tgcattcgct
     7741 cacgaagtac atgggcgggc acggcaattc gctgggcggg atcatcgtgg attcgggcac
     7801 ctttccctgg gcgcagcacg cggcgaagtt cccgatgatg accacgcccg agccttccta
     7861 ccacggcgtg gtgtacacgg aggcgatggg gcccgcggcc tatatcgggc gcgcgcgcac
     7921 ggtgccgctg cgcaacaccg gctcggcgct ctcgccgttc aacgccttcc tgctgctgca
     7981 gggcatcgag acgctggcgc tgcgcatgga gcgccactgc agcaacgcgc tggcggtggc
     8041 gaactacctg cagtcgcacc cgcgcgtgag catcgtgagc tacgccggcc tgcccgggca
     8101 tccgtaccac gcgctggcac agaagtactg cacgggcgga ttgcccgctt cgctgctcac
     8161 cttcgaggtc aaggggggca tggacgaggc ggtgcgcttc tacgacgcgc tgcagctgtt
     8221 caagcggctg gtcaacatcg gcgacgccaa gtcgctggcc tgccacccgg cctcgaccac
     8281 gcaccgccag atgacgcccg aggagcaggc ccgcgccggg gtcacgcccg gcatgatccg
     8341 gctctgcgtc ggcatcgagc acatcgacga catcgtggcc gacctggcgc aggcgctggg
     8401 ctgatcgcgg cccggcgcgc cgagggttct gtccccggcc attgccaggc tggctctcct
     8461 gctcggacac gccataaata cccctccggg gcccagcccg aaatcataga tttcgggcgc
     8521 tcgccggcgc gaagcagtgc ttcgcgtgaa tccgcctcgc ccctgtaggc tcgactgcgg
     8581 catccatgcc gcagacggtc ctcgcaagac agccagcctg gcactggcct cgggctgatt
     8641 c
//
LOCUS       DAORUQ010000134         8547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9098_length_8547_cov_5.983396, whole genome shotgun sequence.
ACCESSION   DAORUQ010000134 DAORUQ010000000
VERSION     DAORUQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9098_length_8547_cov_5.983396"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLW13_11515"
     CDS             complement(<1..1057)
                     /locus_tag="PLW13_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71049.1"
                     /translation="MIANHFLDTDAAAELALFADSVDRFCEARIEPFYAGWEREGQVP
                     RELFRECGEAGLLCVDVPEAWGGLGADPRFSFVIVDVMSRRGYAGFVGGLQVHNDIIP
                     SYLLHCGSEEQKQYWLPKMVSGEVVAAIGMTEPGAGSDLKNIRTVARREGDEYVVNGS
                     KIFISNGQHADLVVLAATIDRGAGSHGVSLFLVDTRTPGFTRGRNLEKVGQHAADTSE
                     LFFDDMRIPASALLGEEGKGFAMMMQELPRERLIVGIQALGLAKGARDLAVRYVQERE
                     AFGQPLAKLQNTRFTLAEAETAIAACEAFANACVEAYARGELTSAAASAFKLHASEMA
                     CRVADDCLQLFGGYGYMA"
     gene            1183..1989
                     /locus_tag="PLW13_11520"
     CDS             1183..1989
                     /locus_tag="PLW13_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQY71050.1"
                     /translation="MHASPLIVQRDGPVLTVTLDRPEKRNPISDPDMVEALVAVMQDA
                     DADLGVRAVILTGAGRAFSSGGDVAKMRAEAGGMRAHLPAQTRRNYKSGIQRLPLVFQ
                     ALEVPVIAAVNGPAIGAGCDLACMCDVRIAAESARFAESFVKLGLIPGDGGAWLLPRI
                     IGFSRATELALTGDQIDAATALEYGLVSRVVPDAQLMDAARAVAMKIAANPPHAVRMT
                     KRLLREAQVSTLPNILELSAAMQGLAHQTTDHDEAIAAFVEKRAPAFRGD"
     gene            2034..2915
                     /locus_tag="PLW13_11525"
     CDS             2034..2915
                     /locus_tag="PLW13_11525"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQY71051.1"
                     /translation="MDYQTLKYDIDDGLLLLTLNRPDHLNAFTVQMADELVAAYERAS
                     LDDAVRVIVVTGAGKAFCAGMDLSVGGNVFGLDESQAPTLQDMRERLEDPAIHRGVRD
                     TGGRVALAMFACRKPIVGAINGAAVGIGATMTLPMDFRFASEKARIGFVFGRLGITPE
                     ACSTWFLPRIVGLEQAMEWMYSARIFDAAEALDRKLVRAVCAPEQLLGEACAFARTLI
                     SERSPVSVALIRQMMYRNSAAPSPLEAHRIESLAMFYSSIGDGKEGVQAFLDKRPPGF
                     SASASEMPPFFAPWSDT"
     gene            2912..4147
                     /locus_tag="PLW13_11530"
     CDS             2912..4147
                     /locus_tag="PLW13_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQY71052.1"
                     /translation="MNAESGATGPLAGIKVLDLSRVLAGPWATQILGDLGAEIIKVEQ
                     PGHGDDTRRWGPPFLQDGSGDAAYYLCANRNKQSIAIDIAHPEGQALIRELARRSDIV
                     VENFRVGGLAKYGLDYASLSAQNPALVYCSITGFGQTGPYKDRGGYDFLIQGMSGLMS
                     ITGRPDGEPGEGPLKVGIPLADLFTGLYATVSILAALGHAKATGTGQHIDCALLDTQV
                     SLLANQASNYLNGGMIPRRLGNEHPNTVPYQDFATADGSILVALGNDRQFRAFCALIG
                     RADLPDDPRFVDNAHRSPNRKALQAEMGAAIATWKSAELLAAMDAAGLPGGRVNSIPD
                     ILADPQIAARELLHHMERSDGTPVAVLGFPARFSGTPANYRRAPPRSGEDTLDVLAHV
                     LRLGDEDIAALRQQGVIPA"
     gene            4257..5204
                     /locus_tag="PLW13_11535"
     CDS             4257..5204
                     /locus_tag="PLW13_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQY71053.1"
                     /translation="MGYDIIGDIHGHARTLEALLETLGYSLVDGAYRHPTRKVVFLGD
                     FIDRGPSQRGVIRIVRPMIDGGHALAVMGNHEFNAIAYATPRAGGGFFREHNAHNYQQ
                     HKVFLDAYEYTQDYHELIDWFGRLPLWLDLEELRIVHACWDHVAIGRIADKYDRYGSL
                     SNSLLYASCTPGNWEFRAVETLLKGKEIPLPRAQSFTDKDGITRHNIRVRWWDRGATN
                     YRDAFLGTESMATAIPEDDIEGDHLVEYSHDDVPVFMGHYWLTGTPAPLAPNIACLDY
                     SVAKPGGKLVAYRWDGERVLDPAKFVAETAVEFALTETL"
     gene            5204..6148
                     /locus_tag="PLW13_11540"
     CDS             5204..6148
                     /locus_tag="PLW13_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015372437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQY71054.1"
                     /translation="MPLPARVRLVEVGPRDGLQNENQPVATTTKVELIRRLGSAGLRS
                     IEATAFVSPTWVPQMADSAAVMAAIERRPGVSYPVLTPNLKGFEAALAAGATEVAVFA
                     AASEAFSQRNTNCSIAESLARFHPVLERARAAAVRVRGYVSCALGCPYEDAVAPARVR
                     DVAGALFEAGCDEISLCDTIGVGTPGATQDLIGCVAARVPLAQLAGHYHDTYGMAIAN
                     IYASLQMGVSVFDSSVAGLGGCPYAVGASGNVATEDVVYLMQGLGIETGVDLEALMDC
                     AEWISAALGRRPDARVTQARRGSGFIQVEPATEREPHR"
     gene            6145..6906
                     /locus_tag="PLW13_11545"
     CDS             6145..6906
                     /locus_tag="PLW13_11545"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71055.1"
                     /translation="MTSFDYTDKVVLITGAASGFGRLAAAAFAEAGALLALADLNLDA
                     LDAAVSDAGIAPERVLTMRCDVAVNSDVQQFVAATVTRFGRLDVAINNAGIGHPLARL
                     EDCDEETFDRNIAVNLKGVFLCLRAELPQMVRQGGGVILNVSSVAGIAGAPMLGPYAA
                     AKHGVIGLTKTVALEYARRNIRVNALCPGYTHTPLLDELRARPGTLDHMAAAIPMQRL
                     AQPGEIVQAMLWACSAQNSFMTGQAIALDGGLTAG"
     gene            complement(6928..8136)
                     /locus_tag="PLW13_11550"
     CDS             complement(6928..8136)
                     /locus_tag="PLW13_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71056.1"
                     /translation="MDFDFSDKSAQLQSRLQAFMDEHVYPAEHAYERQLAAAANRFAM
                     LPLVEELKDKARAAGLWNLFLPAHHGGLSNLDYAPLAEIMGRVLWCPEVFNCSAPDTG
                     NMEVLMKYGTPAQQERWLKPLLAGEIRSAYAMTEPGVASSDATNIETSIRREGEEYVI
                     NGRKWFITNAMYERTKLFIVMGKSDPDNPNRHLQQSQVLVPRETPGLRIVRALTTLGY
                     DDAPQGHAEIVFEDVRVPVANILLGEGRGFEIAQGRLGPGRIHHCMRLVGCAQRALEL
                     ACGRSVARSTFGRKLAEHQSVREDIAKSFCEIEQARLLTLKTAARMDALGAKEARDFI
                     AAAKIAVPLMAQTVIDRCMQIHGAGGLTGDYFMAEAFSYARWCRQADGPDQVHMMALG
                     KQLIARYAGG"
     gene            8281..>8547
                     /locus_tag="PLW13_11555"
     CDS             8281..>8547
                     /locus_tag="PLW13_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71057.1"
                     /translation="MELSGKRAIVVGGASGMALASVELLIRNGVKVAILDLPTSRGAE
                     EAARLGIAFYPCNVTDFAGTEDAIRAAATALGGLHIVVNTAGGGI"
BASE COUNT         1345 a         3063 c         2850 g         1289 t
ORIGIN      
        1 tggccatgta gccgtagccg ccgaagagct gcaggcagtc atcggccacg cggcaggcca
       61 tctcgcttgc gtgcagcttg aacgccgagg ccgccgcgct cgtgagttcc ccgcgcgcat
      121 aggcctcgac gcaggcgttg gcgaaggcct cgcaggccgc gatcgcggtc tcggcctcgg
      181 ccagcgtgaa tcgggtgttc tgcagcttcg cgagcggctg gccgaatgct tcgcgctcct
      241 gcacgtagcg cacggccaga tcccgcgccc ccttggcgag acccagcgcc tggatgccca
      301 cgatcaggcg ctcgcgcggc aactcctgca tcatcatcgc gaagcccttg ccctcctccc
      361 cgagcagtgc cgaggccggg atgcgcatgt cgtcgaagaa gagttccgag gtgtcggccg
      421 cgtgctggcc caccttctcc aggttgcgac cgcgcgtgaa gcccggcgtg cgcgtgtcga
      481 ccaggaacag cgacacgccg tgcgaacccg cgccgcgatc gatcgtcgcg gcgagcacca
      541 ccaggtccgc atgctgcccg ttggagatga agatcttcga gccgttcacc acgtattcgt
      601 cgccctcgcg gcgcgccacg gtgcggatgt tcttcaggtc gctgcccgca cccggctcgg
      661 tcatgccgat ggcggcgacc acctcccccg acaccatctt cggcagccag tactgcttct
      721 gctcctcgct accgcaatgc agcaggtagg acgggatgat gtcgttgtgc acctgcaggc
      781 caccgacgaa gccggcgtag ccgcgtcgtg acatcacgtc cacgatcaca aacgagaagc
      841 gcgggtccgc gccgagcccg ccccaggcct cgggcacatc gacgcagagc agccccgcct
      901 cgccgcactc tcggaacagc tcgcgcggca cctggccctc gcgctcccag ccggcataga
      961 agggctcgat ccgggcttcg cagaaccggt ctacggagtc cgcgaacagc gcgagctcgg
     1021 cggcggcatc ggtgtccagg aaatggtttg caatcatctg gggtctccgt caatctcggt
     1081 ttccggcacg ggccacccgg acccgtttac atttatctta catattatta aattgcgcgg
     1141 gcttttgccc accccgcccg ccatccgcaa caggatctgc cgatgcacgc ttcacccctc
     1201 atcgtacagc gcgatggccc cgtactcacc gtcaccctcg accgccccga gaagcgcaac
     1261 ccgatctcgg acccggacat ggtcgaggcg ctggtcgcgg tcatgcagga tgccgacgcc
     1321 gatctcggcg tgcgcgccgt gatcctgacc ggcgccgggc gggcgttctc ctccggcggc
     1381 gacgtcgcga agatgcgagc ggaggcgggc gggatgcgcg ctcacctgcc ggcgcagacg
     1441 cggcgcaatt acaagagcgg catccagcgc ctgcccctgg tgttccaggc gctcgaggtg
     1501 ccggtgatcg cggccgtcaa cggcccggcc atcggcgccg gctgcgacct cgcctgcatg
     1561 tgcgacgtgc gcatcgcggc cgagagcgca cgcttcgccg agagcttcgt gaagctcggg
     1621 ctcattcccg gcgacggggg tgcctggctg ctgccgcgca tcatcggctt ctcgcgcgcg
     1681 accgagctgg cgctgaccgg cgaccagatc gatgcggcga ccgcgctcga gtacggcctg
     1741 gtctcgcgcg tggtgcccga tgcgcagctg atggacgcgg cgcgggcggt cgccatgaag
     1801 atcgcggcca acccgccgca cgcggtgcgc atgaccaagc gactgctgcg cgaggcgcag
     1861 gtaagcacgc tgccgaacat cctcgagctc tccgcggcga tgcaggggct ggcgcaccag
     1921 accacggacc acgacgaggc catcgccgct ttcgtggaga agcgcgcgcc ggcattccgc
     1981 ggcgattgat tcccgcggca atcatgggcg cgagacgccc gcaaggagtg aacatggact
     2041 accagaccct gaaatacgac atcgacgacg gcctgctgct gctcacgctc aaccggccgg
     2101 atcatctgaa cgccttcacc gtgcagatgg ccgacgagct ggtcgccgcc tacgagcgcg
     2161 ccagcctgga cgatgccgtg cgcgtgatcg tggtcaccgg cgcgggcaag gccttctgcg
     2221 ccggcatgga cctgtcggtg ggcggcaacg tcttcggcct cgacgagagc caggcaccga
     2281 cgctgcagga catgcgcgag cggctggagg atccggcaat ccatcgcggc gtgcgtgaca
     2341 ccggtggccg cgtggcactg gcgatgttcg cctgccgcaa gcccatcgtg ggcgcgatca
     2401 acggcgcggc ggtcggcatc ggcgcgacca tgaccttgcc gatggatttc cgcttcgcct
     2461 cggaaaaggc gcgcatcggc ttcgtgttcg gccgcctcgg catcacgccg gaagcctgct
     2521 cgacctggtt cctgccgcgc atcgtcggcc tggagcaggc catggaatgg atgtacagcg
     2581 cccggatctt cgacgccgcc gaggcgctcg acaggaaact ggtgcgcgcg gtctgcgcac
     2641 cggagcaact gctcggggag gcctgtgcct tcgcgcgcac cctgatcagc gagcgctcac
     2701 cggtcagcgt cgcgctgatc cggcagatga tgtaccgcaa cagcgccgca ccctccccgc
     2761 tggaggcaca tcgcatcgaa tcgctggcga tgttctacag cagcatcggt gacggcaagg
     2821 aaggcgtaca ggcattcctc gacaagcgcc cccccggatt ctcggccagt gcctcggaga
     2881 tgccgccctt cttcgcgccg tggtcggaca catgaacgcc gaatcgggcg cgaccggccc
     2941 cctggccggc atcaaggtgc tcgatctctc gcgcgtgctc gccggcccct gggccacgca
     3001 gatcctcggc gatctcgggg ccgagatcat caaggtcgag cagccgggcc acggcgacga
     3061 cacgcggcgc tggggaccac ccttcctcca ggatggctcg ggcgacgcgg cgtactacct
     3121 gtgcgcgaac cgcaacaagc agtcgatcgc gatcgacatc gcccaccccg aggggcaggc
     3181 gctgatccgc gagctggccc ggcgcagcga catcgtggtg gagaacttcc gcgtcggcgg
     3241 cctggcgaag tacggcctgg actacgcctc gctcagcgcg cagaacccgg cactcgtcta
     3301 ctgctcgatc acgggcttcg gccagacggg accgtacaag gatcgcggcg gctacgactt
     3361 cctgatccag ggcatgagcg ggctgatgag catcaccggc cgccccgacg gcgagcccgg
     3421 cgaaggcccg ctgaaggtgg gcatacccct cgccgatctc ttcaccggac tgtacgccac
     3481 cgtttcgatt ctcgcggcgc tggggcacgc gaaggcgacg ggcaccggcc agcacatcga
     3541 ctgcgcgctg ctcgacaccc aggtgagcct gctggccaac caggcgtcga actacctcaa
     3601 cggcgggatg atcccgcgtc gcctcggcaa cgagcatccc aataccgtgc cgtaccagga
     3661 tttcgcgacc gccgacggca gcatcctggt cgcgctcggc aatgaccgcc agttccgcgc
     3721 cttctgcgcg ctgatcggcc gcgcggacct gccggacgat ccgcgcttcg tcgacaacgc
     3781 ccatcgcagc cccaaccgca aggccctgca ggccgagatg ggagcggcca tcgcaacctg
     3841 gaaatccgcc gagctgctgg ccgcgatgga cgccgccggg ctgcccggcg gccgcgtcaa
     3901 ctccatcccc gacatcctcg ccgacccgca gatcgccgcg cgcgaactgc tccatcacat
     3961 ggaacgcagc gacgggacgc ccgtcgcggt gctcggcttc ccggcccgct tctccgggac
     4021 gccggcgaac tatcgccgcg cgccgccgcg ctcgggcgag gatacgctgg acgtgctggc
     4081 ccatgtgctg cgcctgggcg atgaggacat cgcggcactg cgccaacagg gcgtgatccc
     4141 ggcctgacaa gacccgaacg ggtcatcggt gcagcagcgc tgcggtggtc gcggccagac
     4201 cccgcctccg ctagaatggc ggcgtgaaca gcagcctcag gcagggaact cctctcgtgg
     4261 gatacgacat catcggcgac atccatggtc acgcgcgcac gctggaggcg ctgctcgaga
     4321 cgctgggtta cagcctcgtc gacggcgcat accgccaccc gacgcgcaag gtcgtcttcc
     4381 tcggcgactt catcgaccgc ggcccctcgc agcgcggcgt gatccgcatc gtgcgcccga
     4441 tgatcgacgg cggccacgcg ctggcggtga tgggcaacca cgaattcaac gcgatcgcct
     4501 acgccacccc gcgcgccggc ggcggtttct tccgcgagca caacgcgcac aactaccagc
     4561 agcacaaggt cttcctcgac gcctacgagt acacgcagga ctaccacgag ctcatcgact
     4621 ggttcggccg cttgccgctg tggctggacc tcgaggagct gcgcatcgtg cacgcctgct
     4681 gggaccacgt ggcgatcggg cggatcgcgg acaaatacga ccgctacggc tcgctgagca
     4741 acagcctgct gtatgcctcg tgcacgccgg gcaactggga gttccgcgcg gtggagaccc
     4801 tgctcaaggg caaggaaatc cccctgccgc gcgcgcagag cttcaccgac aaggacggca
     4861 tcacgcgaca caacatccgc gtgcgctggt gggaccgcgg cgccacgaat taccgcgacg
     4921 ccttcctcgg caccgagagc atggccaccg cgatccccga ggacgacatc gagggcgacc
     4981 acctggtcga gtacagccac gacgacgtgc cggtgttcat gggccactac tggctgaccg
     5041 gcacgcccgc cccgctcgcc cccaatatcg cctgcctcga ctacagcgtg gcgaagcccg
     5101 gtggcaagct ggtcgcctac cgctgggacg gcgagcgcgt gctggatccg gcgaagttcg
     5161 ttgccgagac cgcggtcgag ttcgcattga ccgagacgct gtaatgccgc tgcccgctcg
     5221 cgtgcggctg gtcgaggtgg gcccgcgcga cgggctgcag aacgagaacc agccggtagc
     5281 cacgaccacc aaggtggaac tgatccggcg cttgggcagc gcgggcctgc gcagcatcga
     5341 ggcgaccgcg ttcgtatcgc ccacatgggt gccgcagatg gccgacagcg ccgccgtgat
     5401 ggcggcgatc gagcgccggc ccggcgtcag ctaccccgtg ctcacaccca acctgaaggg
     5461 cttcgaggcc gcgctcgcgg ccggcgccac cgaggtcgcc gtgttcgccg ccgccagcga
     5521 ggccttttcg cagcgcaaca ccaattgctc gatcgccgag tcgctggcgc gtttccaccc
     5581 cgtcctggag cgcgcgcgcg cagcggccgt acgcgtgcgc ggctacgtgt cctgcgcgct
     5641 gggttgcccc tacgaggacg cagtcgcgcc cgctcgcgtg cgcgacgtgg ccggcgcgct
     5701 gttcgaggcc ggctgtgacg agatcagcct gtgcgacacc atcggggtcg gcacgcccgg
     5761 cgccacgcag gacctgatcg gctgcgtcgc cgcgcgcgtg ccgctcgcgc agctcgccgg
     5821 ccactaccac gacacctacg gcatggcgat cgcgaacatc tacgcctcgc tgcagatggg
     5881 tgtgagcgtg ttcgacagct cggtggccgg actcggcggc tgcccgtacg cggtgggcgc
     5941 ctccggcaac gtcgcgacgg aggacgtggt gtacctgatg cagggactcg gcatcgagac
     6001 cggggtggac ctcgaggcgt tgatggactg cgccgagtgg atcagtgccg cgctcgggcg
     6061 gcgcccggac gcgcgcgtca cacaggcgcg ccgtgggtcc ggattcatcc aagtcgaacc
     6121 ggcaacagaa cgggagcccc accgatgacg agcttcgatt acacggacaa ggtagtgctg
     6181 atcaccggcg ccgccagcgg cttcgggcgc ctcgcggcgg cggccttcgc cgaggccggc
     6241 gcgctgctgg cgctggcgga cctgaacctg gacgcgctcg atgccgccgt gagcgacgcg
     6301 ggcatcgcgc cggagcgcgt gctcacgatg cgctgtgacg tggccgtgaa cagcgatgtg
     6361 cagcagttcg tggccgccac cgtgacgcgc ttcggccgtc tggacgtcgc gatcaacaac
     6421 gccggcatcg gccacccgct ggcgcggctc gaggactgtg acgaggaaac cttcgaccgc
     6481 aacatcgcgg tgaacctgaa gggggtgttc ctgtgcctgc gcgccgaact gccgcagatg
     6541 gtgcgccagg gcggcggcgt gatcctcaac gtgagctcgg tcgcaggcat cgccggggcg
     6601 ccgatgctcg ggccctacgc cgcggccaag cacggcgtga tcgggctcac gaagaccgtc
     6661 gcgctcgaat acgcgcggcg aaatatccgc gtgaacgcgc tgtgccccgg ctacacgcac
     6721 acgccgctgc tcgacgaact ccgcgcccgc cccggaacgc tggatcatat ggccgccgcc
     6781 atcccgatgc agcgcctcgc gcagcccggg gagatcgtgc aggccatgct gtgggcctgc
     6841 tcggcgcaga acagcttcat gaccggccag gccatcgcgc tggacggcgg cctgaccgcg
     6901 ggctgaaccg gcgttgccgc tgccggctca gccgccggcg tagcgcgcga tcagctgctt
     6961 gcccagcgcc atcatgtgca cctggtccgg gccgtcggcc tggcggcacc agcgcgcgta
     7021 gctgaaggct tcggccatga agtaatcgcc ggtgagcccg cccgcgccgt ggatctgcat
     7081 gcagcggtcg atcacggtct gcgccatcag cggcaccgcg atcttcgccg ccgcgatgaa
     7141 atcccgtgcc tccttggcac cgagcgcgtc catgcgcgcg gcggtcttca gcgtcagcag
     7201 gcgcgcctgc tcgatctcgc agaacgactt cgcgatgtcc tcgcggaccg actggtgctc
     7261 ggcgagcttg cgcccgaagg tgctgcgcgc caccgatcgc ccgcaggcga gctccagcgc
     7321 gcgctgcgcg cagccgacca ggcgcatgca gtggtggatg cggcccggcc cgaggcgacc
     7381 ctgcgcgatc tcgaagccgc gaccctcgcc gagcaggatg ttcgccaccg gcacgcggac
     7441 gtcctcgaac acgatctccg cgtgtccctg cggcgcgtcg tcgtagccca gcgtggtgag
     7501 cgcgcgcacg atgcgcagcc ccggcgtctc gcgcggcacc agcacctggc tctgctgcag
     7561 gtgccggttc gggttgtcgg ggtcggactt gcccatgacg atgaacagct tcgtgcgctc
     7621 gtacatcgcg ttggtgatga accacttgcg cccgttgatc acgtactcct cgccttcgcg
     7681 ccggatcgag gtctcgatgt tggtggcatc gctcgaggcc acgccgggct ccgtcatcgc
     7741 gtaggccgag cggatctcgc cggccagcag cggcttcagc cagcgctcct gctgcgccgg
     7801 cgtgccgtac ttcatcagca cttccatgtt gccggtgtcg ggcgcgctgc agttgaacac
     7861 ctcggggcac cacagcacgc ggcccatgat ctcggccagc ggcgcatagt ccaggttcga
     7921 caggccgcca tgatgcgcgg gcaggaacag gttccacagc cccgcggcac gcgccttgtc
     7981 cttcagttcc tccaccagcg gcagcatcgc gaagcggttc gcggccgccg cgagctggcg
     8041 ttcgtatgcg tgctccgccg gatacacgtg ctcgtccatg aatgcctgca ggcggctctg
     8101 cagttgcgcg ctcttgtcgc tgaagtcgaa gtccacgctt gcctcccggc tcgttgtcga
     8161 tggtgctgcc gtggcgccgc tgcgccatgc cagatcaggg atcttaagat aacatcagcg
     8221 ccgccggtac ggcgctgccg tccccgcgtc ccgggccatc caatcgagga gaacaggatc
     8281 atggaactga gtggaaagcg cgcaatcgtc gtcggtggcg catcgggcat ggcgctcgcg
     8341 agcgtcgaac tgctgatccg caacggcgtg aaggtcgcca ttctcgacct gccgacgtcc
     8401 cggggagccg aggaagccgc gcgactcggc atcgcctttt acccctgcaa cgtcacggac
     8461 ttcgccggca ccgaggacgc gatccgcgcc gccgccaccg cgctgggcgg gctgcacatc
     8521 gtggtcaata ccgcgggcgg cggcatc
//
LOCUS       DAORUQ010000135         8495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9177_length_8495_cov_6.959123, whole genome shotgun sequence.
ACCESSION   DAORUQ010000135 DAORUQ010000000
VERSION     DAORUQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_8495_cov_6.959123"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLW13_11560"
     CDS             <1..711
                     /locus_tag="PLW13_11560"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQY71058.1"
                     /translation="ARFRAIADKVGAYLLVDMAHVAGLVAAGLYPNPVPHADVVTTTT
                     HKTLRGPRGGLIIARKNDEITKKLNSAVFPGGQGGPLMHVIAAKAVSFKEALEPEFKV
                     YQQQVVDNARAMARTFIERGIDIVSGGTDNHLMLVNLIGKPYTGADADAALGEANITV
                     NKNAVPNDPRPPAVTSGLRVGTPAITTRGFGIDETVQLTHWMCDVLASLEDGSSERVI
                     AEVRAKVLDICKRYPVYA"
     gene            815..2494
                     /locus_tag="PLW13_11565"
     CDS             815..2494
                     /locus_tag="PLW13_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQY71059.1"
                     /translation="MNKNNGQPTLRAVAAIAGLCLLALLPLHGASAATREDAALDRHL
                     ALEVETSPVMLDGEVLFRVRGTSLVPPAKRAGAIAERLKWLADNESIPVDALHIESDA
                     ELSRILAGEQLIMVLADIDAEGVPRDRLAELVMSDMSKAVQAYRAARAPRVLLQHTGY
                     VVLATLAAAALLYGLLRLRRWTLARLRGRFVAGGADLKIQSVQLVQSRQVWLAVRGLV
                     NAAITVSVLAVIYLYLSTVLGLYPWTAPLADQLFQLFLQPLQVMGGAVLASLPDLGFI
                     VVLVVVVRFVLKALQLMFQGIETGAVSFENFDPEWAQPTYKIVRILVIAFAVVVAYPY
                     IPGSGSQAFKGVSLFLGVIFSLGSSSVISNVIAGYTMTYRRAFRLGDRIAVGEITGFV
                     TETSLLVTRLRSFKNEEVVLPNSEILGKAVINYSALARREGLILHTTVGIGYETPWRQ
                     VEAMLLLAAERTAGLEREPRPFVLQKSLGDFCVVYEINATTRDATQIGLLYSALHRNI
                     LDVFNEYGVQIMTPAYEGDPEVPKLVPREQWHLAPAAPEPAGPTPVGQLSG"
     gene            complement(2543..4558)
                     /locus_tag="PLW13_11570"
     CDS             complement(2543..4558)
                     /locus_tag="PLW13_11570"
                     /inference="COORDINATES: protein motif:HMM:NF021639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71060.1"
                     /translation="MAAGTDGFSAALATALAAVRALEPGADALPALAALTAALRPRNS
                     REAASRVRWLELLRQLEQEPATQLALREALLGFFAARRQVAFFSDSGLLPNSGFFSEA
                     ARILGHKLLPEPRDRDDLRSGIRELFPTARDAAWIGTISDDERHAFWRLLRLSGSENA
                     NALQQFIAQLLDAALVLSHRICGMGLEPELARVCPRLLEPGVESPFIALNAELLGVVE
                     GIRHTLLGDEERDHGGHVFVLLEQCAEVVQRARQASATFGTSMRLSFILVRLTQHLER
                     LRLLLEVLTLQTRPDAPAALSASLGNFLHAALAGELRRNSLRRHAAQLLGLVALRITG
                     NAARTGEHYIATDRADWIAMWRRAGGAGLLIALLALLKILASTLHLPPVTQAWLNAGI
                     YAGGFALIHMLHFVIATKQPSMTAATLAAAISQAGGRLRDTERIVDLLAATLRSQLAA
                     IAGNVLVALPVAVLVGLALAQQAPPPVSAAKATRLLAELDAVRSPLVAHAAIAGIWLF
                     LTGLVSGHLDNRAASMRLEERIAQLRWLGAVLGGRRAARIGRYLAEHAGGLGGNLFFG
                     LMLGLTPAAGLLLGLPLDIRHVAFSSANLGYALAATGFAPPEGTLARACAGVVLIGFI
                     NLAVSFGLALWVAMRSHGIGFARLAPVLPRLAARLRSDPRSFVAPPA"
     gene            complement(4566..6980)
                     /locus_tag="PLW13_11575"
     CDS             complement(4566..6980)
                     /locus_tag="PLW13_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71061.1"
                     /translation="MNAPTRRAPRPAAGLTLLAAATLAASAAAQEAPRDGAAMALEEI
                     VVTARMREESLQNAPIAISAYTGASLAYRGVSRLDDAAKFVPGLTLENNPSFGGASNS
                     AAIYLRGVGQKEFLPTTDPGVGLYVDGVYVARSVGAILDVVDIERVEVLRGPQGTLFG
                     RNTIGGAISVTTVKPEPGGEFGGYVSGATGTDDLLRLKGSVHLPLGETLAARLSVADL
                     NQDGYVERTDGVDLGDDDTTTGRAALAWHPNERFAADLAFEATRDRENGQPMRLIGID
                     FTDLSQLQGLVASVPPPMAFIHNVTFGATAPGVPCAATDAAGNGVTYNPAAPNCYDAR
                     YIGKDGKNQGTAPAFSEADLRGASATLRYELTPALTLKSISAWRDLDAEFARDGDDSP
                     HRISQFYDELGQEQYTQELQLLGSHERLNWILGLYYFTEEGENTNILDFTVSNFRSGG
                     DFENESWAAFAQATYDITSTLHLTLGARQTEEEKSFRPDQVIFQNYFAGISQVVPPGN
                     PLAALDAPFLQAGERVLPYLWKDIDTSEFTPMANLSWDAREDLMLYLGYSEGFKSGGF
                     TQRVFPPIVAGYTAPPGTPDIDLIPTYDPEFVDVWELGFKWSGLDGQLRLNGAAFRTE
                     YEDMQVQVFDSVAPVTRNIGKATIDGLELELQAAPGAGWLIDASATWLDPGYDEIDTA
                     NTLIGEDYEFERVPEYAASLGVSKEFDLESLGFVTVRGDWSYRDATYNDAYNTPLLQT
                     DSYDLIDASVRWQDCQRLWTVALSGRNLGDEEYLVTGVYGTAFQSLEGTFDRGRQWQL
                     ELRRDF"
     gene            complement(7020..8384)
                     /locus_tag="PLW13_11580"
     CDS             complement(7020..8384)
                     /locus_tag="PLW13_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQY71062.1"
                     /translation="MSSDYTKILLEEHEMPTRWYNIVPDLPEPPPPPLHPGTLKPATA
                     DDFKALFPPALIAQEMSTERYIDIPPEVLDVYRLWRPSPLFRARRLEKLLKTPAKIFY
                     KYEGVSPAGSHKPNTAVPQVWYNAQAGIRKLTTETGAGQWGSSLAFACAQFGLECEIW
                     QVAASYRAKPYRRTMMEIWGGKVHPSPSTVTAFGRSLLAQDPEHPGSLGIAISEAVSE
                     ALKDPHTRYALGSVLNHVLLHQTVIGEEALLQLKKVGETPDVLVGCTGGGSNFGGLAF
                     PFLREKLAGRMNPVIRCVEPTACPTLTRGEYRYDFGDTEGLTPLMKMHTLGAHFIPEP
                     IHAGGLRYHGMAPLVSHVYHLGLVEAIAIPQTECFAGAIQFARTEGIVPAPEPTHAIA
                     AAVREAIACRESGEPKVILTALCGHGHLDLASYDKYLHGGMQDLDLSDEVLAEAMRHV
                     PEIA"
BASE COUNT         1262 a         2926 c         2991 g         1316 t
ORIGIN      
        1 gcgcgctttc gcgccatcgc cgacaaggtc ggcgcctacc tgctggtcga catggcccac
       61 gtggcgggcc tggttgccgc cggcctctac ccgaacccgg tgccgcacgc cgacgtggtc
      121 accaccacca cgcacaagac gctgcgcggc ccgcgcggcg ggctgatcat cgcgcgcaag
      181 aacgacgaga tcaccaagaa gctgaactcc gcggtgttcc ccggcggcca gggcggcccg
      241 ctgatgcacg tgatcgcggc caaggccgtg agcttcaagg aagcgctgga gccggaattc
      301 aaggtctacc agcagcaggt cgtggacaac gcgcgcgcga tggcgcgcac cttcatcgag
      361 cgcggcatcg acatcgtctc gggcggcacc gacaaccacc tgatgctggt gaacctgatc
      421 ggcaaaccct acaccggcgc cgatgccgat gcggcgcttg gcgaggcgaa catcacggtg
      481 aacaagaacg ccgtgccgaa cgatccgcgc ccgccggcgg tgaccagcgg cctgcgcgtg
      541 ggcacgcccg cgatcaccac gcgcggcttc ggcatcgacg agacggtgca gctcacgcac
      601 tggatgtgcg acgtgctggc ctcgctcgag gacggcagca gcgagcgcgt gatcgccgag
      661 gtgcgcgcga aggtcctcga catctgcaag cgttaccccg tctacgcctg accgcactgt
      721 ccggctgaag ccggacctgc gggtgcccct tttcctgcgc gtggggcggc gtgctaggct
      781 ggtgccggtc gcgttcacaa cggatcgttc caccatgaac aagaacaatg gacagccgac
      841 gttgcgcgcc gttgccgcga tcgcgggact ttgcctgctc gccctgcttc ccctgcacgg
      901 cgcgagcgcc gcgaccaggg aggacgcggc gctggaccgc catctggccc tcgaggtcga
      961 gacctcgccg gtgatgctcg atggcgaggt gttgtttcgc gtccgcggca cctccctggt
     1021 gccgcctgcc aagcgcgccg gcgcgatcgc cgaacgcctc aagtggctcg ccgacaacga
     1081 gtcgataccc gtcgacgccc tgcacatcga gtccgatgcc gagctgtcgc gcatcctcgc
     1141 cggtgagcag ctcatcatgg tgctcgccga catcgacgcc gagggcgtgc cccgcgaccg
     1201 cctggccgaa ctcgtcatgt cggacatgag caaggccgtg caggcctacc gcgccgcgcg
     1261 cgccccgcgc gtgctgctgc agcacaccgg ctatgtcgtg ctggcgacac tggcggccgc
     1321 cgcgctgctg tacgggctgt tgcggctgcg acgctggacg ctggcgcggt tgcgcgggcg
     1381 cttcgtcgcc ggcggagcgg atctgaagat ccagtcggtg cagctcgtgc agtcgcgcca
     1441 ggtgtggctg gcggtgcgcg gcctggtcaa tgccgcgatc acggtgtcgg tgctcgcggt
     1501 gatctacctg tacctcagca cggtgctggg cctgtatccg tggacggccc cgctggccga
     1561 tcaactgttt cagcttttcc tgcagccgct gcaggtgatg ggcggcgccg tgctggcttc
     1621 gctccccgat ctcggcttca tcgtggtgct cgtggtggtc gtgcgcttcg tgctgaaggc
     1681 actgcagctc atgttccagg gcatcgagac cggcgcggtc agcttcgaga acttcgaccc
     1741 cgagtgggcg cagcccacct acaagatcgt gcgcatcctc gtgatcgcct tcgccgtggt
     1801 ggtcgcgtac ccgtacatac cgggctccgg gtcgcaggcc ttcaagggcg tgtcgctgtt
     1861 cctgggggtg atattttcgc tgggctcctc gtccgtgatc tcgaacgtga tcgccggtta
     1921 caccatgacc taccgtcgcg ccttccgcct gggcgatcgc atcgccgtgg gcgagatcac
     1981 cggcttcgtg accgaaacct cgctgttggt gacgcgcctg cgctcgttca agaacgaaga
     2041 agtggtgttg ccgaactcgg agatcctcgg caaggccgtc atcaactaca gcgcgctggc
     2101 acgccgcgaa ggcctgatcc tgcacaccac ggtgggcatc ggctacgaga cgccgtggcg
     2161 ccaggtcgag gcaatgctgc tgctggcggc cgagcgaacc gccgggctcg agcgcgaacc
     2221 gcgccccttc gtgctgcaga agtcgctggg cgacttctgc gtcgtctacg agatcaatgc
     2281 gaccacgcgg gacgcaacgc agatcggtct gctgtactcc gcgctgcaca ggaacatcct
     2341 cgacgtcttc aacgagtacg gcgtgcagat catgacgccg gcctacgagg gcgatcccga
     2401 ggtgccgaag ctggtgccgc gcgagcagtg gcacctggcg cctgccgcac ccgaacccgc
     2461 aggtccgact ccagtcggac aattgtccgg ataaatcctg acccacggga cccgactgtc
     2521 cggctggagc cggacctacg cgctacgccg gcggcgccac gaagctgcgc gggtcgctgc
     2581 gcagccgcgc cgcgaggcgc ggcagcaccg gcgccaggcg ggcgaaaccg ataccgtgcg
     2641 agcgcatcgc tacccacagc gcgagaccga agctcacggc caggttgatg aagccgatca
     2701 gcaccacgcc ggcgcaggcg cgcgcgagcg tcccctcggg cggtgcgaag cccgtggcgg
     2761 cgagcgcgta gccgaggttt gccgaggaga acgccacgtg acggatgtcg agcggcaggc
     2821 ctagcagcag gcccgccgcg ggcgtcaggc cgagcatcag gccgaagaac aggttgccgc
     2881 cgagtccacc cgcatgctcc gccaggtagc ggccgatgcg cgcggcgcgc cgcccgccga
     2941 gcacggcacc gagccagcgc agctgcgcga tgcgctcctc gagccgcatc gaggccgcgc
     3001 ggttgtcgag gtggcccgag accagcccgg tcaggaacag ccagatgccc gcgatcgcgg
     3061 catgcgccac cagcgggctg cgcaccgcat cgagctcggc gagcaggcgc gtcgccttgg
     3121 ccgcggacac cggtggcggc gcctgttgcg ccagcgccaa tcccaccagc accgccacgg
     3181 gcagcgccac cagcacgttg ccggcgatcg cggcgagctg gctgcgcagc gtcgcggcca
     3241 gcaggtcgac gatgcgctcg gtgtcgcgca gccgcccgcc ggcctggctg atggccgccg
     3301 cgagcgtggc ggccgtcatc gacggctgct tggtggcgat cacgaagtgc agcatgtgga
     3361 tcagcgcgaa gccgcccgcg tagatgccgg cgttcagcca cgcctgcgtg acgggtggca
     3421 ggtgcagggt gctggccagg atcttcagca gcgcgagcag cgcgatcagc aggccggcac
     3481 cgccggcgcg gcgccacatc gcgatccagt cggcgcgatc ggtcgcgatg tagtgttccc
     3541 ccgtgcgcgc ggcgttgccc gtgatgcgca gcgccaccag cccgagcagt tgcgcggcgt
     3601 gacggcgcag gctgttgcgc cgcagctcgc cggcgagcgc ggcgtggaga aagttgccca
     3661 ggctcgcgct cagcgccgcc ggcgcatcgg gacgcgtctg cagcgtgagc acttccagca
     3721 gcagccgcag gcgctccagg tgctgcgtga gccgcacgag gatgaacgac aggcgcatgc
     3781 tggtgccgaa ggtcgccgac gcctggcggg cacgctgcac cacctcggcg cactgctcca
     3841 gcagcacgaa gacatgcccg ccgtgatcgc gctcctcgtc gccgagcagg gtgtggcgaa
     3901 tgccctccac cacgccgagc agctccgcgt tgagcgcgat gaagggcgac tccaccccgg
     3961 gctcgagcag ccgcggacac acccgcgcga gctccggctc gaggcccatg ccgcagatgc
     4021 ggtgcgacag caccagcgcc gcatccagca gctgggcaat gaactgctgc agcgcgttgg
     4081 cgttctccga gccgctgagg cgcagcagcc gccagaaggc atgccgctcg tcgtcgctga
     4141 tcgtgccgat ccacgcggca tcgcgggccg tgggaaacag ctcgcgaatg ccggagcgaa
     4201 gatcgtcgcg gtcgcgcggc tcgggcagca gcttgtgccc gaggatccgc gcggcctcgc
     4261 tgaagaaccc gctgttgggg agcaggcccg aatcgctgaa gaatgccacc tggcgccgcg
     4321 cggcgaagaa acccagcagc gcctcgcgca gggcgagctg cgttgccggc tcctgctcca
     4381 gttgccgcag cagttccagc cagcgcacgc gcgatgccgc ctcgcgcgag ttgcgcggac
     4441 gcagcgccgc ggtgagcgcg gcgagcgcgg gcagcgcatc ggccccgggc tccagcgcgc
     4501 gcacggccgc caacgccgtc gcgagcgccg cggagaaccc gtcagtgccc gcggccatcg
     4561 tcacttcaga aatcgcggcg cagctccagc tgccactggc ggccgcgatc gaaggtgcct
     4621 tccagcgact ggaacgcggt gccgtagacg ccggtcacca ggtactcctc gtcgccgagg
     4681 ttgcgccccg acagcgccac cgtccacagc cgctggcagt cctgccagcg cacgctggcg
     4741 tcgatgaggt cgtaggagtc cgtctgcagc agcggcgtgt tgtaggcgtc gttgtaggtc
     4801 gcgtcgcggt agctccagtc gccgcgcacg gtcacgaagc cgagcgattc gaggtcgaac
     4861 tccttcgaca cgcccaggct cgcggcgtac tccggcacgc gctcgaactc gtagtcctcg
     4921 ccgatcagcg tattggcggt gtcgatctcg tcgtagcccg ggtcgagcca ggtggcgctg
     4981 gcgtcgatca gccagcccgc gcccggcgcg gcctgcagct cgagctcgag accgtcgatg
     5041 gtcgccttgc cgatgttgcg cgtcaccggc gccacgctgt cgaagacctg cacctgcatg
     5101 tcctcgtatt cggtgcggaa cgccgcgccg ttcaggcgca gctgcccgtc gagcccgctc
     5161 cacttgaacc caagctccca cacgtcgacg aactcggggt cgtaggtcgg gatcaggtcg
     5221 atgtcgggcg tgccgggcgg cgcggtgtag ccggccacga tcggggggaa cacgcgttgc
     5281 gtgaagccac cggacttgaa gccctccgag tagcccaggt agagcatcag gtcctcgcgg
     5341 gcgtcccacg acagattcgc catcggcgtg aactccgagg tgtcgatgtc cttccacagg
     5401 tagggcagca cgcgctcgcc cgcctgcagg aagggcgcat cgagcgccgc cagcgggttg
     5461 cccggcggca ccacctggct gatgcccgcg aagtagttct ggaagatcac ctggtcgggg
     5521 cggaaggact tctcctcctc ggtctggcgc gcgccgagcg tcaggtgcag cgtgctcgtg
     5581 atgtcgtagg tcgcctgcgc gaaggctgcc cacgactcgt tctcgaagtc gccgccggag
     5641 cggaagttcg agaccgtgaa gtcgaggatg ttggtgttct cgccttcctc ggtgaagtag
     5701 tagagcccca ggatccagtt cagccgctcg tgcgagccga gcagctgcag ctcctgcgtg
     5761 tactgctcct gcccgagctc gtcgtagaac tgcgagatgc ggtgcggcga gtcgtcgccg
     5821 tcgcgcgcga actcggcgtc gagatcgcgc caggcgctga tcgacttcag cgtgagcgcg
     5881 ggcgtcagtt cgtagcgcag cgtggccgag gcgccgcgca ggtcggcctc cgagaaggcc
     5941 ggcgccgtgc cctggttctt gccgtccttg ccgatgtagc gcgcgtcgta gcagttgggc
     6001 gccgccgggt tgtaggtgac gccgttgccg gcggcatcgg tcgcggcgca gggcacgccc
     6061 ggcgcggtcg cgccgaaagt gacgttgtgg atgaatgcca tcggcggcgg caccgaggcc
     6121 accaggccct gcagctggct gaggtccgtg aagtcgatgc cgatcaggcg catcggctgg
     6181 ccgttctcgc gatcgcgcgt ggcctcgaag gcgagatcgg ccgcgaagcg ctcgtttgga
     6241 tgccacgcga gcgccgcgcg cccggtggtg gtgtcgtcgt cgccgagatc cacgccgtcg
     6301 gtgcgctcga cgtagccatc ctggttcagg tcggccaccg agagccgcgc ggccagcgtc
     6361 tcgccgagcg gcaggtgcac cgagcccttc aggcgcagca ggtcgtcggt gccggtggcg
     6421 ccgctgacgt agccgccgaa ctcgccgccc ggctccggct tcacggtggt gaccgagatc
     6481 gcgccgccga tggtgttgcg cccgaacagc gtgccctgcg ggccgcgcag cacctcgacg
     6541 cgctcgatgt cgaccacgtc gaggatcgcg cccactgagc gcgcgacgta gacgccgtcg
     6601 acgtagaggc ccacacccgg atccgtggtc ggcaggaact ccttctggcc cacgccgcgc
     6661 aggtagatcg ccgccgagtt cgaggcgccg ccgaagctcg ggttgttctc cagcgtcagg
     6721 cccggcacga acttcgcagc atcgtccagc cggctcacgc cgcggtaggc gagcgatgcg
     6781 ccggtgtagg cggagatggc gatcggggcg ttctgcaggc tttcctcgcg catgcgcgcg
     6841 gtgaccacga tctcttccag tgccatcgcg gcgccatcgc gcggcgcctc ctgggcggcg
     6901 gcgctggcgg ccagggtcgc ggccgccagc agggtgagtc cggccgcagg ccgcggcgct
     6961 cgtcgggtgg gtgcgttcat ggctgtgttt cccctttcta ttttctatct attttctgtt
     7021 caggcgatct cgggcacgtg gcgcatcgct tcggcgagca cctcgtcgga gagatcgaga
     7081 tcctgcatgc cgccgtgcag gtacttgtca taggaggcca ggtccaggtg cccgtggccg
     7141 cagagcgcgg tgaggatcac cttcggctcg ccgctctcgc ggcaggcaat ggcctcgcgc
     7201 accgccgcgg cgatcgcgtg cgtgggctcg ggcgccggca cgatgccctc ggtgcgcgcg
     7261 aactggatcg cgcccgcgaa gcactcggtc tgcgggatcg cgatcgcctc gaccaggccc
     7321 aggtggtaga cgtgcgagac cagcggcgcc atgccgtggt agcgcagccc cccggcgtgg
     7381 atcggctcgg ggatgaagtg cgcgcccagc gtgtgcatct tcatcagcgg ggtcaggccc
     7441 tcggtgtcgc cgaagtcgta gcggtactcg ccccgcgtga gcgtggggca ggcggtgggc
     7501 tccacgcagc ggatcaccgg gttcatgcgc ccggccagct tctcgcgcag gaaggggaac
     7561 gccaggccgc cgaagttcga gccgccgccg gtgcaaccga ccagcacgtc cggcgtctcg
     7621 ccgaccttct tcaattgcag cagcgcctcc tcgccgatca ccgtctggtg cagcagcacg
     7681 tggttcagca cgctgccgag cgcgtagcgc gtgtgcgggt ccttcagcgc ctcgctcacc
     7741 gcctcggaga tggcgatgcc gagcgagccg gggtgctcgg gatcctgcgc cagcagcgag
     7801 cgcccgaacg cggtgacggt cgaggggctc gggtgcacct tgccgcccca gatctccatc
     7861 atcgtgcggc ggtagggctt ggcgcggtac gaggccgcca cctgccagat ctcgcactcc
     7921 agcccgaact gcgcgcaggc gaaggcgagc gagctgcccc actgcccggc gccggtctcg
     7981 gtggtgagct tgcggatgcc cgcctgcgcg ttgtaccaga cctgcggcac cgcggtgttg
     8041 ggcttgtgcg agcccgccgg gctcacgccc tcgtacttgt agaagatctt cgccggcgtc
     8101 ttcagcagtt tctcgagacg gcgcgcgcgg aacagcgggc tcggccgcca caggcgatag
     8161 acgtcgagca cctcgggcgg gatgtcgatg tagcgctcgg tgctcatctc ctgcgcgatc
     8221 agcgccggcg ggaacagcgc cttgaaatcg tccgcggtcg ccggcttgag cgtgcccggg
     8281 tgcagcggcg gcggcggggg ctcgggcagg tccggcacga tgttgtacca gcgcgtcggc
     8341 atctcgtgtt cttcgaggag gatcttggtg tagtcgctgc tcatggcatc cgggtccgtt
     8401 gtgcgttgct gtgaagtggc gtcagccgcc gcgggcgaat cataagcaaa atcgcatcaa
     8461 ccggagggaa tcagggggat taaattttga tcaag
//
LOCUS       DAORUQ010000136         8433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9284_length_8433_cov_5.353664, whole genome shotgun sequence.
ACCESSION   DAORUQ010000136 DAORUQ010000000
VERSION     DAORUQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9284_length_8433_cov_5.353664"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="trpB"
                     /locus_tag="PLW13_11585"
     CDS             complement(<1..1199)
                     /gene="trpB"
                     /locus_tag="PLW13_11585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQY71063.1"
                     /translation="MGLFDFAPGREGYYGKYGGAFLPEILHSTIEELSAGFRACKNDP
                     AFWQEYQQLMREYSGRPTPVTYLANLSRQLGGARIYVKREDLNHTGSHKINNVMGQGL
                     LCKRLGKTRVIAETGAGQHGFATATMAAKFGFRCRIYMGAVDIARQRPNVFWMENLGA
                     EVVSVEDGQRTLKDAINEAMRDWATNMADTHYVLGTACGAHPFPEMVSWFQSIIGLEA
                     REQILAAAGKLPDRVYACVGGGSNAIGLFQGFLDDAGVELVGCEAGGFGPGKGLHASR
                     LAYHDASVGVAQGFKTFFLQDDQGNMLETHSVAAGLDYIGVNPILVWLWEQGKVRFEA
                     ATDAEVAETLRLVMRTEGLIPALESTHAFVRAVKEAPAMGKDEVILINQSGRGDKDIF
                     TVAKHLG"
     gene            complement(1261..1833)
                     /locus_tag="PLW13_11590"
     CDS             complement(1261..1833)
                     /locus_tag="PLW13_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQY71064.1"
                     /translation="MNRFCSNCGAALAAPPDGTTRCAACGALALPGPKVLVSCFVFQG
                     DRLLWMRRAHDPQRGFWAVPAGFMELGETLEQAAARELREETGLALDPSVLRLYSLGS
                     ITQINEVYVAFRASVGEACCAPGPEALDVRFFSEAELPWDQVAFPRANYSIQMAYDDI
                     RRGRFGLYLAQHDRGELVDQFEHLVESDRC"
     gene            complement(1830..2243)
                     /locus_tag="PLW13_11595"
     CDS             complement(1830..2243)
                     /locus_tag="PLW13_11595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HQY71065.1"
                     /translation="MSNYKSFGKFHTWVYRLSGGKVLGTLGLGRQVLLLTTTGRKSGA
                     RRTTPLIYMPDGERFVLYGSNGGQENPPAWLLNLEADPRADVEIGRRRIPVRAHIAEG
                     AEHERLLPVAHAYNPHWKGYQEHAQRRIPLVVLTP"
     gene            complement(2344..3066)
                     /locus_tag="PLW13_11600"
     CDS             complement(2344..3066)
                     /locus_tag="PLW13_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71066.1"
                     /translation="MVALVAGAAGGIGAALGAEILERYRLRRLFVVSRGDAPAGFAAE
                     RVVALRADLLAPGATVELARSVAGFEPRLDLVFNCCGLLHDGALKPEKSVAAIDAGAL
                     ERLFAVNATLPALLARDLGPLLRRSPDAVFASLSARVGSIADNRAGGWYGYRASKAAH
                     NMLLRTLALEWRHAQPRTVCLMLQPGTVVTPLSRPFLSARTHEHAMTPAQAARHLLDV
                     VERVAPADSGSFLDWQGRAVPW"
     gene            complement(3098..3397)
                     /locus_tag="PLW13_11605"
     CDS             complement(3098..3397)
                     /locus_tag="PLW13_11605"
                     /inference="COORDINATES: protein motif:HMM:NF039469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6482 family protein"
                     /protein_id="HQY71067.1"
                     /translation="MVDLLIPSRIAAAEVRAACIRSLEGSIYLVELECAGGRRTLGDG
                     RGRAVFRSLPAARAALAGLGAIPVRLVHDSVYDQMIGLGAGVPNRLEVALGEAGA"
     gene            3461..4033
                     /locus_tag="PLW13_11610"
     CDS             3461..4033
                     /locus_tag="PLW13_11610"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQY71068.1"
                     /translation="MPAWLFATAHRALLMATLVLSAAPAVTAPAQPAPLATVPAVDIA
                     RYAGLWYEIAAIPMWFQRNCLGDTTARYTPNPDGTIDVLNRCRTSEGTDEAHGLARIV
                     DTQGNARLEVSFFSILGWRPVWGDYWIIALAPDYSWAVVGTPERRYGWILARTPALEA
                     ATLATIRQRLEELGYDPASFVPTAQQAAPR"
     gene            4035..4493
                     /locus_tag="PLW13_11615"
     CDS             4035..4493
                     /locus_tag="PLW13_11615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY71069.1"
                     /translation="MDGWDHAHPFLHAVRVQPAHIDVLAHANNIAYIGWCQDAAWRHS
                     AALGLAPDAYQTLDRAMVVRKARYEYLLAAMAGDELDVGTWLTGSDGRLQMTRHFQVR
                     RGADGATLFRGDWELVCIRISNGRPVRMPQVFMDSYLPAVIDTSGNAAPG"
     gene            complement(4470..4679)
                     /locus_tag="PLW13_11620"
     CDS             complement(4470..4679)
                     /locus_tag="PLW13_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71070.1"
                     /translation="MAEQQAAVIDERDELRKNILLLVRYCAFIGPSSNAEIVLHVLAE
                     VDRLKARYRDLDPGRTGGLSRSGVS"
     gene            complement(4719..5549)
                     /locus_tag="PLW13_11625"
     CDS             complement(4719..5549)
                     /locus_tag="PLW13_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71071.1"
                     /translation="MGALDARIAVVTGASRGIGVGIARRFAAEGATVVVVARTLEAHD
                     KLPGSLRETVAMIERDGGRAHALTADLTDPGSRRGLIERCAQAAGAAPHILVNNAAAA
                     IYLPLAGYPAKRFNLSIELNLHAPLDLAQQCLPAMMERGEGWILNISSATAKHPARPY
                     LPWDVAGGSGIYGASKAALNRMSAALAAELLQHNIAVNTLAPVSAVLTPGVEMLGIDQ
                     SDPNFRTEPVEAMAEAALVLCSERAAVRTGETTYSVEFLRRIGRTVRGLDGTGEISID
                     "
     gene            5630..6403
                     /locus_tag="PLW13_11630"
     CDS             5630..6403
                     /locus_tag="PLW13_11630"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71072.1"
                     /translation="MTMPQSVFDMSGRRVLITGGGTGLGQRFAHTLAAAGATVILAAR
                     RADRLEQTARSLRERGASAHCVTIDVASAASVANAFRAIAAIGPLDVLVNNAGTAADK
                     SLLDIGEDEWDRVMNANLKGAWLVARAAATAMIAAGTGGAIVNIASVLGSAVQKHTGP
                     YSAAKAGLLHLTRAMALEWARHGIRVNAIAPGYYRTDMAADFLASDAGRLLQKRIPQR
                     RLGDPQELDGAILLLASGASSYMTGSVITVDGGLSLAVV"
     gene            complement(6437..7504)
                     /gene="corA"
                     /locus_tag="PLW13_11635"
     CDS             complement(6437..7504)
                     /gene="corA"
                     /locus_tag="PLW13_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQY71073.1"
                     /translation="MSYLGKSYHPPGTAPGTLDDRKRGAIEIRLVDYDGTDYVERTLE
                     RAEDCTPYLDRVSRTWIQVNGRPDADTLRSLGRLFDLHELALEDVLNTGQRPKLDIFG
                     DQLFVVLAMPVHRNGSVVSEQVSIFAGPDYLVCFSTTDADPFEPLRKRMRPPSTSRLR
                     SQGIDYLLYGIVDFVIDAAFPVLEEFGVEIEQLEEVLLAAPGRDAIARIHHIRRELLL
                     LRRMLWPQRELVSRLQRDEIELISDSTMPFLRDCYDHCVQIIDLLESFREMSASLLEV
                     YLSSISNRTNEVMRVLTIISTIFIPLTFIVGVYGMNFDTERSPWGMPELGWYYGYPLV
                     WGVMIVIAATLMWFFRRKHWL"
     gene            7635..8402
                     /locus_tag="PLW13_11640"
     CDS             7635..8402
                     /locus_tag="PLW13_11640"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HQY71074.1"
                     /translation="MNSEDKAQAPPAIRSVAAGRGLDWITGGWSLFLRAPGPWIALTV
                     LVFVVSMICTVVPVLGNLLSPFVSAVLSGGALLAARNVQGSGTLQVADLFAIFSHPAL
                     KSVLIVAGIYVGLILVAVMVFAGITIGVGGVAMLAGAMAGDASATAGGLLGVVFAALV
                     FLALMAPITAMYWFAIPAVLFQGAEPWSAMKQSLSACLANLVSMLVYGVLGLIAFSVA
                     MIPFMLGLLIVVPVLFASWLLSYQDIFGVESPIPPTA"
BASE COUNT         1316 a         2997 c         2818 g         1302 t
ORIGIN      
        1 aacccaaggt gtttggccac ggtgaagatg tccttgtcgc cgcgcccgga ctggttgatc
       61 aggatcacct cgtccttgcc catcgcgggc gcctccttca cggcgcgcac gaaagcatgc
      121 gtgctctcga gcgcgggaat caggccttcc gtgcgcatca ccaggcgcag cgtctcggcg
      181 acctcggcat cggtcgcggc ctcgaagcgc accttgccct gctcccacag ccacaccagg
      241 atcgggttca ccccgatgta gtcgaggccc gccgcgaccg aatgggtctc cagcatgttg
      301 ccctgatcgt cctgcaggaa gaaggtcttg aagccctggg ccacgccgac cgaggcgtcg
      361 tggtaggcga ggcgcgaggc gtgcaggccc ttgccgggcc cgaagccgcc ggcctcgcaa
      421 ccgaccagct ccacgccggc atcgtcgagg aaaccctgga acagcccgat agcgttcgaa
      481 ccgccaccga cgcaggcata gacgcgatcg ggcagcttgc ccgcggccgc gaggatctgc
      541 tcgcgcgcct cgaggccgat gatcgactgg aaccagctca ccatctccgg gaagggatgc
      601 gcgccgcagg ccgttcccag cacgtagtgc gtgtcggcca tgttggtggc ccagtcgcgc
      661 atcgcctcgt tgatcgcgtc cttcagcgtg cgctggccgt cctcgaccga gaccacttcg
      721 gcaccgaggt tctccatcca gaacacgttg gggcgctggc gcgcgatgtc gaccgccccc
      781 atgtagatgc ggcaacggaa gccgaacttc gccgccatcg tggccgtggc gaagccgtgc
      841 tggcccgcgc ccgtctccgc gatcacgcgg gtcttgccaa ggcgtttgca cagcaggccc
      901 tgccccatca cgttgttgat cttgtgcgag ccggtgtggt tcaggtcttc gcgcttgacg
      961 tagatccgcg cgccacccaa ctggcgcgaa aggttcgcga ggtaggtcac cggcgtcggg
     1021 cgccccgagt actcgcgcat cagctgctgg tactcctgcc agaaggccgg gtcgttcttg
     1081 caggcgcgaa agcccgcgct gagctcctcg atggtcgagt gcaggatctc gggcaggaac
     1141 gcgccgccgt acttcccgta atagccctcg cgtccggggg cgaaatcgaa caggcccatg
     1201 cgctggaact cccgtgaaaa gccgtcaagg atacgcaaaa gcgcgccgcg cagaaaccgc
     1261 tcagcagcgg tcggattcca ccagatgctc gaactggtcg accagttcgc cgcgatcgtg
     1321 ctgcgcgaga taaagtccga agcgcccgcg gcggatgtcg tcgtaggcca tctggatcga
     1381 ataattggcg cgcggaaagg caacctggtc ccacggcagc tcggcttcgg agaaaaagcg
     1441 cacgtccagc gcctccggtc cgggcgcgca gcaggcctcg ccgaccgagg cgcggaacgc
     1501 gacgtagacc tcgttgatct gcgtgatcga gccgaggctg tagagcctga gaacggacgg
     1561 atcgagcgcc agtccggtct cctcgcgcaa ctcgcgcgcg gcggcctgct ccagcgtctc
     1621 accgagctcc atgaagccgg cgggcaccgc ccagaaaccg cgctgcgggt cgtgcgcgcg
     1681 acgcatccac aacagcctgt cgccctggaa cacgaaacag gacaccagca ccttgggccc
     1741 gggcagggcg agcgcgccgc aggccgcgca gcgcgtcgtg ccgtccggcg gcgcggccag
     1801 cgcggcgccg cagttgctgc agaagcggtt cacggcgtca gcacgacgag cgggatgcgc
     1861 cgctgcgcgt gctcctggta gcccttccag tgcggattgt aggcgtgcgc cacgggcagc
     1921 agccgctcgt gctcggcgcc ctcggcgatg tgcgcccgca cggggatgcg gcgccgcccg
     1981 atctcgacgt cggcgcgcgg atcggcctcg aggttcagca gccacgccgg cgggttctcc
     2041 tgcccgccgt tggagccgta gagcacgaag cgctcgccat cgggcatgta gatcagcggc
     2101 gtggtgcgcc gcgcaccgct cttgcgcccg gtggtcgtga gcagcaggac ctggcgcccg
     2161 agccccaggg tgccgaggac cttgccgccg ctcagccggt aaacccaggt gtggaatttt
     2221 ccgaaggact tgtagttcga catcgcgacc atccgttgct gttgccgcgg cgcccgaccg
     2281 tgccggcgcc gcgtggggcg ccaccttagc ggatatcgcc ccaacaatca tccgcgccgc
     2341 cgctcaccac ggcaccgccc gaccctgcca atcgaggaag ctgccggagt ccgccggtgc
     2401 cacgcgttcg accacgtcga gcaggtgccg ggcagcctgc gccggcgtca tcgcgtgctc
     2461 gtgcgtgcgg gcactcagga acggccgcga tagtggcgtg acgaccgttc cgggctgcag
     2521 catcaggcac accgtgcgcg gctgcgcatg gcgccactcc agtgccagcg tgcgcagcag
     2581 catgttgtgc gccgccttgc tcgcgcggta gccgtaccag ccgcccgcgc ggttgtccgc
     2641 gatgctgccg acccgcgccg acaggctcgc gaataccgca tcgggcgagc gccgcagcag
     2701 cggcccgagg tcgcgcgcga gcaacgcggg cagcgtcgcg ttgaccgcga acaggcgctc
     2761 gagcgccccg gcgtcgatgg cggccacgct cttctccggc ttcagggcgc cgtcgtgcag
     2821 cagcccgcag cagttgaaca ccaggtccag ccgcggctcg aagccggcga cgctgcgcgc
     2881 cagttccacc gtcgctccgg gcgcgagcag gtcggcgcgc aacgccacca cgcgctctgc
     2941 ggcgaaacca gccggcgcat ctccccgcga gaccacgaac agccgcctca gccggtagcg
     3001 ttcgagaatc tccgcgccga gcgcggcgcc aatgccaccc gcagcccctg ccaccagtgc
     3061 gaccatcccg tcaccgtggc tgcgccatgc gcgcgcctca ggcaccggct tcaccgagcg
     3121 cgacttcgag ccggttgggg acgcctgcgc ccagaccgat catctggtcg tagacgctgt
     3181 cgtgaaccag gcgcaccggt atggcgccta gacccgccag cgcggcacgc gcggccggca
     3241 agctgcggaa aaccgctcgc ccccggccgt caccgagcgt gcgccggcca ccggcgcact
     3301 ccagttcgac gaggtagatg ctgccctcta gcgaacggat gcacgccgct cgcacctcgg
     3361 ctgcggcaat gcgggacggg atcaagagat cgaccatggg attcactccg tgatgacgct
     3421 ggtgtacggt gagcgccact ttccggatcg gatcccgcac atgcctgcct ggcttttcgc
     3481 gaccgcgcac cgcgccctgc tcatggcaac cctcgtcctg tccgcggcgc cggcggtcac
     3541 ggcgcccgcg caacccgcgc cactggcgac ggtgccggcg gtcgacatcg cccgctacgc
     3601 cgggctctgg tacgagattg cggcgatccc gatgtggttc cagcgcaact gcctcggcga
     3661 taccaccgcg cgctataccc cgaatccgga cggcacgatc gacgtgctga atcgctgccg
     3721 caccagcgag ggcaccgacg aggcacacgg actcgcgcgc atcgtcgata cgcaaggcaa
     3781 cgcgcgcctg gaggtgagct ttttcagcat cctgggctgg cgtccggtgt ggggcgacta
     3841 ctggatcatc gcgctcgcgc ccgactacag ctgggccgtg gtcggcacgc ccgagcgccg
     3901 ctacggctgg atcctggcgc gcacgccagc gctggaagcc gcgacgctgg ccacgatccg
     3961 ccaacggctc gaggaactgg gctacgatcc cgcgagcttc gttcccaccg cgcagcaggc
     4021 cgcgccacgc tgaaatggac ggctgggatc acgcgcatcc cttcctgcac gccgtgcgcg
     4081 tgcagcccgc acacatcgat gtgctcgcgc acgccaacaa catcgcctac atcggctggt
     4141 gccaggacgc tgcgtggcgg cattcggcgg cgctgggcct ggcaccggac gcctaccaaa
     4201 cgctcgatcg cgcgatggtg gtgcgcaagg cgcgctacga atacctgctc gcggccatgg
     4261 caggcgatga actcgacgtc ggcacctggc tcaccggcag cgacggccgg ctccagatga
     4321 cgcgtcactt ccaggtgcgc cgcggcgccg acggggcgac gctgtttcgt ggcgactggg
     4381 aactggtgtg catccggatc tccaacggca ggccggtgcg catgccgcag gtattcatgg
     4441 acagctacct gcccgcggtg atcgacacct caggaaacgc cgctcctgga taacccgccc
     4501 gtgcgccccg gatcgaggtc gcgatagcgt gccttcagcc ggtccacctc ggccagcacg
     4561 tgcagcacga tctcggcgtt gctgctcggc ccgatgaagg cgcaatagcg caccagcagg
     4621 aggatgttct tcctgagctc gtcacgctca tcgatgaccg ctgcctgctg ctctgccatg
     4681 tgcgtccccc cgcaaggcgc gcgccgattg tagcgcgctc agtcgatgct aatctccccc
     4741 gtgccgtcca ggccgcgcac cgtgcgaccg atccggcgca ggaattcgac gctgtaggtg
     4801 gtctcgccgg tccgcaccgc tgcgcgctcg ctgcagagca ccagagccgc ctcggccatg
     4861 gcctccaccg gctcggtgcg gaaattcggg tccgactggt cgatgcccag catttccacg
     4921 cccggcgtga gcaccgccga gaccggcgcc agcgtgttca ccgcgatgtt gtgctgcagc
     4981 agctcggcag ccagcgcagc cgacatgcgg ttcagcgccg ccttcgaggc gccatagatc
     5041 cccgagccgc cggccacgtc ccagggcagg taggggcgcg ccggatgctt cgcagtcgcc
     5101 gagctgatgt tcaggatcca gccctcgccg cgctccatca tcgcgggcag gcactgctgc
     5161 gcgaggtcga gcggcgcgtg caggttcagc tcgatcgaca ggttgaagcg cttggcggga
     5221 tacccggcca gcggcaggta gatcgcggcg gcggcgttgt tcaccaggat gtgcggcgca
     5281 gcgccggcgg cctgtgcgca gcgttcgatg agcccccggc gcgagccggg atcggtgagg
     5341 tccgcggtga gcgcatgcgc ccggccgccg tcgcgctcga tcatcgcgac cgtttcgcgc
     5401 agggaacccg gcagcttgtc gtgcgcctcc agcgtccggg ccacgaccac caccgtggcg
     5461 ccctcggcgg cgaagcggcg cgcgatcccc accccgatgc cacggctcgc gccggtgacc
     5521 accgcaatcc ttgcgtccag cgctcccatc gtgcttctcc tgccgcgaac cctgcgttaa
     5581 attcccggca gcttaagcat tcaaccgatc cggggccaga gaggcgacca tgacgatgcc
     5641 gcaatccgtg ttcgacatga gcgggcgacg cgtgctgatc accggcggcg gcaccgggct
     5701 gggacagcgc tttgcgcaca ccctcgcggc cgcgggcgcc accgtgatcc tggccgcgcg
     5761 gcgtgcggac aggctggagc agacggcgcg cagcctgcgc gagcgcggcg ccagcgcgca
     5821 ctgcgtgacc atcgacgtcg ccagcgccgc gagcgtcgcg aacgccttcc gggcgattgc
     5881 cgcgatcggc ccgctcgacg tgctggtgaa caacgcgggg acggccgccg acaagtcgct
     5941 gctggacatc ggcgaagacg aatgggaccg ggtcatgaac gccaacctca agggcgcctg
     6001 gctggtggcc cgcgccgccg ccacggcgat gatcgccgcc ggcacgggcg gcgcgatcgt
     6061 gaacatcgcc tcggtgctgg gctctgccgt ccagaagcac accggcccct actccgccgc
     6121 caaggcggga ctgctgcatc tcacgcgcgc gatggcgctg gaatgggcgc gccacggcat
     6181 ccgcgtcaac gcgatcgcgc cgggctatta ccgcaccgac atggcggcgg atttcctcgc
     6241 atccgacgcg ggcaggctgc tgcagaaacg catcccgcaa cggcgcctgg gcgatccgca
     6301 ggagctcgac ggcgcgatcc tgctgctcgc ctccggggct tcctcctaca tgaccggctc
     6361 ggtgatcacg gtcgatggcg ggctttcgct ggcggtggtg taggtcggct gaagccggac
     6421 ccgcgcccga gccgcattac agccagtgct tgcgccggaa gaaccacatc agggtcgccg
     6481 cgatcacgat catcacgccc cacaccagcg gatagccgta gtaccagccg agctccggca
     6541 tgccccaggg cgagcgctcg gtgtcgaagt tcatgccgta gacgcccacg atgaaggtca
     6601 gcgggatgaa gatcgtcgag atgatcgtga gcacccgcat cacctcgttg gtgcggttgc
     6661 tgatgctcga gagatagacc tcgagcaggc tcgccgacat ctcgcgaaag ctctcgagca
     6721 ggtcgatgat ctgcacgcaa tggtcgtagc agtcgcgcag gaacggcatc gtgctgtcgc
     6781 tgatcagctc gatctcgtcg cgctgcagcc ggctcacgag ctcgcgctgc ggccacagca
     6841 tccgccgcag cagcagcagt tcgcgcctga tgtggtgaat ccgcgcgatc gcatcgcgac
     6901 cgggcgcagc cagcaacacc tcctcgagct gctcgatctc gacgccgaac tcctcgagca
     6961 ccgggaacgc cgcatcgatc acgaaatcga cgatcccgta gagcaggtaa tcgatgccct
     7021 ggctgcgcag gcgcgacgtc gagggcggcc gcatgcgctt gcgcagcggc tcgaagggat
     7081 ccgcgtccgt agtcgaaaag cagaccaggt aatcggggcc ggcgaagatg ctgacctgtt
     7141 cgctcaccac gctgccgttg cgatgcaccg gcatcgcgag caccacgaag agctgatcgc
     7201 cgaagatgtc cagcttcggg cgctgtcccg tgttcagcac gtcttccagc gcgagctcgt
     7261 gcaggtcgaa caggcgcccg aggctgcgca gggtgtcggc atcgggccgg ccgttgacct
     7321 ggatccaggt gcgcgagacg cgatcgagat acggcgtgca gtcctcggca cgctccagcg
     7381 tgcgctcgac gtagtcggta ccatcgtaat ccacgagccg gatctcgatc gcgccgcgct
     7441 tgcgatcatc gagcgtgccc ggcgccgtgc ccggcggatg gtagctcttg cccagatagc
     7501 tcatggcgtc attccgcggc gattcatccc gcgaagctta gcgcatggct gccgcgactg
     7561 cccccgttgc cgcgcgtgct attctcaatc gcgatgcagc gcatccgtgc ccgcccgcca
     7621 aggagaacaa ccgcatgaac agcgaagaca aggcgcaagc ccctcccgcc atacgcagcg
     7681 tcgccgccgg ccgcggcctc gactggataa ccgggggctg gtcgctgttc ctgcgcgcgc
     7741 ccgggccgtg gatcgcgctg acggtgctgg tgttcgtggt ctcgatgatc tgcaccgtgg
     7801 tgccggtgct cggcaacctg ctcagcccct tcgtctcggc cgtgctttcg ggcggcgccc
     7861 tgctggcggc tcgcaacgtg cagggtagcg gcacgctgca ggttgccgac ctgttcgcga
     7921 tcttctcgca cccggcgctg aagtccgtgc tgatcgtggc gggcatctac gtcgggctga
     7981 tcctggtcgc ggtgatggtg ttcgccggga tcacgatcgg ggtgggcggc gtggcgatgc
     8041 tcgccggcgc gatggccggt gatgcgtccg ccaccgccgg cggcctgctc ggcgtcgtct
     8101 tcgcggccct ggtgttcctc gcactgatgg cgccgatcac ggcgatgtac tggttcgcga
     8161 tccccgccgt gctgttccag ggcgccgagc cgtggagcgc gatgaagcag agcctgtcgg
     8221 cctgcctggc gaacctggtg tcgatgctgg tctacggcgt gctcggcctg atcgcgttca
     8281 gcgtcgccat gattccgttc atgctcggcc tgctcatcgt ggtgccggtg ctgttcgcct
     8341 cgtggctgct cagctaccag gacatcttcg gcgtggaatc tccaataccc cccactgctt
     8401 gaaccagcga cccggcgcgg cgagggacgg gcc
//
LOCUS       DAORUQ010000137         8424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9297_length_8424_cov_6.264309, whole genome shotgun sequence.
ACCESSION   DAORUQ010000137 DAORUQ010000000
VERSION     DAORUQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9297_length_8424_cov_6.264309"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="PLW13_11645"
     CDS             complement(<1..1810)
                     /locus_tag="PLW13_11645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQY71075.1"
                     /translation="MSIEALKVRTTSRSAEVPAALQPASEDIWDRKYRLKDKAGNPLD
                     ETIDGTYRRVAQALSEVESEEQRQHWYERFVWALRNGAIPAGRIISNAGAQEHKPATS
                     TINCTVSGTIEDSMLGILEKNLEAGLTLKAGCGIGYEFSTLRPKGAYVSGAGAYTSGP
                     LSFMDIFDKMCFTVSSAGGRRGAQMGTFDISHPDVVEFIRAKRENGRLRQFNLSLLVT
                     EEFMQAVARDEDWPLSFPISAKELEDGDYDIGNSDEVIWRHFPHARGYVSDAEGRVAC
                     RIYKSVKARKLWNVIMISTYDFAEPGFILIDKVNEQNNNWFCENIRATNPCGEQPLPP
                     YGSCLLGSVNLTRFVQAPFREDARFDWTTFREVVAVFTRMLDNVVEMSGLPLEQQRHE
                     IENKRRHGMGFLGLGSTLTMLRIKYGSTEAVEFTERVSRELALTGWRTGLELAREKGP
                     APIMNEAFTVTAEMLAKRPEMVRDGFKVGDLVPGRILHARYSRYMQRVASVEPELVEA
                     LAQEGCRFTHHSSIAPTGTISLSLANNASNGIEPSFAHQYSRNVIREGKKSKEKIDVF
                     SYELLAYRELVNARATPYSSDPDEQLPEYFLTSEDIA"
     gene            2127..2318
                     /locus_tag="PLW13_11650"
     CDS             2127..2318
                     /locus_tag="PLW13_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71076.1"
                     /translation="MRASALGLPLLCCCIAGARAIGPGLREEFAIHQAGIYAVGAVDA
                     GGGEAMVGPVHDMALDTNA"
     gene            2315..3085
                     /locus_tag="PLW13_11655"
     CDS             2315..3085
                     /locus_tag="PLW13_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQY71077.1"
                     /translation="MTRQLRILTYNIHKGYCSGNRRFVLEGMRALIAETGADLVFLQE
                     IHGRHSGHARHEGRYSYPEQPHFEYLADQSWPHYAYGRNAIYRRGDHGNAILSKFPFV
                     RWENINVALFPRSSRSILHGVIEIPESGFRLHTLCVHLGLLEQERRGQLETLAERIES
                     HVPHDEPLIIAGDFNDWRRRAGRHLHASLGVDELFLELEGRHARSFPVWQPLLAMDRI
                     YYRGVRPLSCQRLSAGPWRELSDHAALMGEFSLPRKRR"
     gene            complement(3088..6195)
                     /locus_tag="PLW13_11660"
     CDS             complement(3088..6195)
                     /locus_tag="PLW13_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQY71078.1"
                     /translation="MPDRHPKMSYAELHCISNFSFLRGASHPEELVAEAARQGYRALA
                     ITDECSLAGVVKAHVAAREHGLHLIIGAEFRLDGELLLVLLAPDRRAYAELSGLITLA
                     RRRSPKGEYAVQLRDFEVNVRHCLAIWLPGTAAIEPLQRQGEALGTLFAGRLWLGVEL
                     LLGAGEQQRYLRLYQLAARLGLPMVACGGVEMHVAQRKALADTLCAIRLNTPVQQLGT
                     RLAANAERHLRALERLRRLYPAALLAESVRIAQRCSFTLGELRYEYPEEVVPPGMTPT
                     AWLRRLVLAGAQQRWPDGVPRRAQALIEKELVLIAELAYEYYFLTVHDLVQFARGNGI
                     LCQGRGSAANSAVCYCLFITEVDPDRSSVLFERFISKERNEPPDIDVDFEHERREEVI
                     QYIYRKYTRERAALAATIISYRPRSAVRDVGKALGLDPLFVEQLAKSLSWWDRHGDLA
                     RRFEEEHLAGASSMAGHFMRLVREILGFPRHLSQHVGGFIITRSPIAALVPVENAAMM
                     DRTVIQWDKNDIEALGLLKVDILALGMLSALRRAFALIRAAHGVEIDMSRIPAEDPCT
                     FAMLARADSVGVFQVESRAQMAMLPRLKPRTFYDLVIEVAIVRPGPIQGDMVHPYLKR
                     RQGLEPVTYPNEEVRAVLERTLGVPIFQEQVIKLAMVAAGFSGGEADQLRRAMAAWKR
                     NGTLAAFEDKVINGMLARGHSREFAERLFNQMKGFGDYGFPESHAASFALLAYASAWL
                     KCHYPAAFCCALLNSQPMGFYSPSQLVQDLRRHGVTVLPVDVDRSEWDCALERCDGQS
                     PALRLGLRQISGFNAAAAGRIVAARRRRAFDDLHDLARRALLDRSELDLLAGAGALVR
                     LGGHRHQAHWQAAAIEEPRPLFDRAETAQLLHDAVELPAPGEAEDLVQDYRSIGLTLG
                     RHPLALLRARQPFAGCCSAHQLHALNSGRFVRVAGLVTGRQRPGTASGVLFLTLEDET
                     GNINVVVWRDRQERFRKALLRGKLLLVKGVMESRDSVVHVIAGEILDHSAALGALQTR
                     SRDFH"
     gene            6281..6733
                     /locus_tag="PLW13_11665"
     CDS             6281..6733
                     /locus_tag="PLW13_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HQY71079.1"
                     /translation="MAAPRAAPFWSSKTLDQLNREEWESLCDGCGLCCLQKLEDEYTR
                     RVYYTNVACKLLDLESCQCSNYARRQEYVPDCIALTPGDADAYRWLPETCAYRLVAQG
                     DELPPWHHLVCGDRGAVHRAGISRAGQMLPEAAVPEDAWEEHIIFRIG"
     gene            6788..7960
                     /locus_tag="PLW13_11670"
     CDS             6788..7960
                     /locus_tag="PLW13_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71080.1"
                     /translation="MSLDFDSARLRDPNLRPEHEEWRTQLRRFIDREIMPRVDEWDEA
                     CRIPDELWPKAAAIGLLGLGYPEAYGGTAEGIDIYHLNIVAEELARTSLGGINSTLLV
                     HGIGLPPVVNFASDALKEAVIPPILRGEKRISLAITEPSGGSDVANIQTTARRDGEHY
                     IVNGAKTFISGGMGANWFTTAVRTGGEGGRGISVLLIPADSAGVARTPLARKQGWWCS
                     DTATIHFDEVRVPAANLVGPENMGFLVLMANFNHERMAMAVGMEAFARVCLEEAVSWA
                     RERRTFGKRLADHQVIRHKIAMMKQRINATQAYIQMCSRQIVDGTVQPGDIALLKVQA
                     SETMEFCAREAMQVLGGAGYLRGNRVERIYREVRVNAIGGGSEEIMRDLAARQFGF"
     gene            8113..>8424
                     /locus_tag="PLW13_11675"
     CDS             8113..>8424
                     /locus_tag="PLW13_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71081.1"
                     /translation="MRFTEEHQAIRATTAKFIDAEINPHADEWEAAGIFPAHEVFRKM
                     GALGLLGIAKPAECGGLGLDYSYQIVFSEELGRIKSGGVGMGIGVQTDMATPALAKFG
                     SD"
BASE COUNT         1358 a         2805 c         2872 g         1389 t
ORIGIN      
        1 gggctatgtc ctcgctggtg aggaagtact ccggcagctg ctcatcggga tcgctcgagt
       61 acggcgtggc gcgcgcgttc accagctcgc ggtaggccag cagctcgtag gagaacacgt
      121 cgatcttctc cttggatttc ttgccctcgc ggatgacgtt gcgcgagtac tggtgcgcga
      181 aactcggctc gatgccgttc gaggcgttgt tggcgagcga cagcgagatc gtgccggtcg
      241 gcgcaatcga ggagtggtgc gtgaagcggc agccctcctg ggcaagtgct tccaccagct
      301 ccggttccac cgaggcgacg cgttgcatgt agcggctgta gcgcgcgtgc aggatacgtc
      361 cgggcacgag atcgccgacc ttgaagccgt cacgcaccat ttccgggcgt tttgccagca
      421 tttccgccgt caccgtgaac gcctcgttca tgatcggcgc gggtcctttc tcgcgcgcca
      481 gctcgagccc ggtgcgccag cccgtcagcg cgagctcgcg cgagacgcgc tcggtgaact
      541 ccaccgcctc ggtgctgccg tacttgatgc gcagcatcgt cagggtggac ccgaggccga
      601 ggaaacccat gccgtggcgg cgcttgttct cgatctcgtg gcgttgctgc tcgagcggca
      661 ggccgctcat ctccaccacg ttgtcgagca tgcgtgtgaa caccgccacc acctcgcgga
      721 aggtggtcca gtcgaagcgg gcgtcctcgc gaaacggcgc ctgcacgaag cgggtgaggt
      781 tcaccgagcc gagcaggcag ctgccgtagg gcggcagggg ctgttcgccg caggggttgg
      841 tggcgcggat gttctcgcag aaccagttgt tgttctgctc gttgactttg tcgatcagga
      901 tgaagcccgg ctcggcgaag tcgtaggtcg agatcatgat gacgttccag agcttgcgcg
      961 ccttcacgct cttgtagatc cggcaggcga cgcgcccctc ggcgtcgctg acgtatcccc
     1021 gggcgtgcgg gaaatgccgc cagatcacct cgtcgctgtt gccgatgtcg tagtcgccgt
     1081 cctcgagttc cttggccgag atcggaaagg acaggggcca gtcctcgtcg cgcgccaccg
     1141 cctgcatgaa ttcctccgtc accagcagcg acaggttgaa ctggcgcagg cgcccgttct
     1201 cgcgcttggc gcggatgaac tcgaccacgt ccgggtggct gatgtcgaag gtgcccatct
     1261 gcgccccgcg gcgcccgccg gccgaggaca cggtgaaaca catcttgtcg aagatgtcca
     1321 tgaacgacag cgggccgctg gtgtaggccc cggcgcccga gacataggcg cccttggggc
     1381 gcagcgtcga gaattcgtag ccgatgccgc aaccggcttt aagcgtcagg cccgcctcga
     1441 ggttcttctc gaggatgccg agcatcgagt cctcgatggt gcccgaaacg gtgcagttga
     1501 tggtcgaggt cgcgggcttg tgctcctggg cgccagcgtt ggaaatgata cgcccggccg
     1561 ggatcgcgcc attgcgcaag gcccacacga aacgctcgta ccagtgctgg cgctgctctt
     1621 cggactccac ttcggacagc gcctgtgcca cccggcgata ggtgccgtcg atggtttcgt
     1681 ccagcgggtt acccgccttg tccttcaggc ggtacttgcg atcccagatg tcttccgagg
     1741 ccggttgcag ggccgcgggg acctccgcgc tgcggctggt ggttctgacc ttgagtgctt
     1801 ctatggacat gtgcgcctcg ctcgtattct tggccggttg attttgcgag agtttttctg
     1861 gttggtcgtt gccgacgcat gcaaatcccc cgcatcggca gatgacgtca tcgtgcgttc
     1921 ggggaatcaa aactcaagat gcagtgtaat ggaggtacat caaaacacaa tatgtagtgt
     1981 caaagttttt tggcctgcct gccgttggca gggactttgc acggtcccgg cgctccctcc
     2041 gggcctccgt cgtaggggga gcatattgcc tgcccgaagc ccttttcgct agcatccgcc
     2101 ccgcttgcct ctacgggaat tcctccatgc gtgcatcggc cctcggcctc ccgctgcttt
     2161 gctgctgcat tgcgggtgcg cgcgccatcg ggcccgggtt gcgcgaggag ttcgccatcc
     2221 accaggccgg aatctacgcg gtcggcgcgg tcgatgccgg ggggggcgag gcgatggtcg
     2281 gtcccgtgca cgacatggcc ctggacacca acgcttgacg cgacagctgc gcatactcac
     2341 ctacaacatc cacaagggct attgctccgg caaccgccgt ttcgtcctcg agggtatgcg
     2401 ggcgctgatc gccgagacgg gcgccgacct cgtgttcctg caggagatcc atggccgcca
     2461 cagcggccat gcccgccacg aggggcgcta cagctatccc gaacagccgc atttcgagta
     2521 tctcgccgac cagtcgtggc cgcactacgc ctacgggcgc aatgccatct accgcagggg
     2581 cgatcacggc aacgcgatcc tcagcaagtt tcccttcgtg cgctgggaga acatcaacgt
     2641 cgcgctgttt ccgcgctcca gccgcagcat cctgcacggg gtgatcgaga tccccgagag
     2701 cggctttcgc ctgcacacgc tgtgcgtgca cctcgggctg ctcgagcagg agcgccgcgg
     2761 ccagctcgag acgctcgccg aacgcatcga gtcgcacgtg ccgcacgacg agccgctgat
     2821 cattgccggc gacttcaacg actggcgccg ccgcgccggg cgccacctgc acgccagcct
     2881 cggggtggac gagctgttcc tcgagctcga ggggcggcat gcgcgcagct tcccggtctg
     2941 gcagccgctg ctcgcgatgg accggatcta ctatcgcggg gtgcggccgc tctcgtgcca
     3001 gcgtctcagt gccgggccct ggcgcgagct ctcggaccac gcggcgctga tgggggagtt
     3061 cagtctcccg cgcaagcgtc gctgatctca gtgaaaatcg cgtgagcgtg tctgcagcgc
     3121 gcccagcgcc gcgctgtggt cgagaatctc gccggcgatc acgtggacca cgctgtcgcg
     3181 gctctccatc acgcccttga ccagcagcag cttcccgcgc agcagcgcct tgcgaaagcg
     3241 ttcctggcgg tcgcgccaga ccacgacgtt gatgttgccg gtctcgtctt ccagcgtgag
     3301 gaacagcacg ccgctcgcgg tgccggggcg ctggcggccg gtgaccagtc ctgccacgcg
     3361 cacgaaacgg ccgctgttca gcgcgtgcag ctggtgcgcg ctgcagcagc ccgcgaaggg
     3421 ctgacgcgcg cgcagcagcg ccagcggatg gcgccccagc gtgaggccga tgctgcgata
     3481 gtcctgcacc aggtcctcgg cctccccggg cgcgggcagc tcgaccgcat cgtgcagcag
     3541 ctgcgcggtc tcggcgcggt cgaacagggg acggggctcc tcgatcgccg cggcctgcca
     3601 gtgcgcctgg tggcggtggc cgccgaggcg caccagcgcg ccggcgccgg ccagcaggtc
     3661 cagctcgctg cgatcgagca gtgcgcggcg cgcgagatcg tgcaggtcgt cgaaggcccg
     3721 gcgccggcgc gcggcgacga tgcgcccggc cgcggcagcg ttgaagccgc tgatctggcg
     3781 caggccgagg cgcagcgcgg ggctttgccc gtcgcacctc tccagcgcgc agtcccactc
     3841 gctgcggtcc acgtcgaccg gcagcaccgt gacgccgtgg cggcgcaggt cctgcaccag
     3901 ctgcgagggt gagtagaagc ccatcggctg gctgttcagc agcgcgcagc agaaggcggc
     3961 cgggtagtgg cacttcagcc aggccgaggc gtaggcgagc agcgcgaagc tcgccgcgtg
     4021 cgactcggga aagccgtagt ccccgaagcc cttcatctgg ttgaacaggc gctcggcgaa
     4081 ctcgcggctg tggccgcgtg cgagcatgcc gttgatgacc ttgtcctcga aggcggcgag
     4141 cgtgccgttg cgcttccagg ccgccatcgc acggcgcagc tggtcggcct cgccgccgct
     4201 gaacccggcc gcgaccatcg cgagcttgat cacctgctcc tggaagatcg gcacgcccag
     4261 ggtgcgttcc agcacggcgc gaacctcctc gttgggatag gtgacgggct cgagtccctg
     4321 gcggcgcttc aggtaggggt ggaccatatc gccctggatc ggccccgggc gcacgatcgc
     4381 gacctcgatc accaggtcgt agaaggtgcg cggcttcagt cgcggcagca tggccatctg
     4441 cgcgcgcgat tcgacctgga acacgcccac gctgtcggcg cgcgcgagca tcgcgaaggt
     4501 gcagggatcc tcggccggga tacggctcat gtcgatctcc acgccgtgcg cggcgcggat
     4561 caaggcgaag gcgcgccgca gcgcgctcag catgccgagc gcgaggatgt cgaccttcag
     4621 cagacccagc gcctcgatgt cgttcttgtc ccactggatc acggtgcgat ccatcatcgc
     4681 ggcattctcg accggcacca gcgccgcgat ggggctgcgc gtgatgatga agccgccgac
     4741 atgctgcgag aggtgacgcg ggaaaccgag gatctcgcgc accaggcgca tgaaatgccc
     4801 ggccatgctg ctcgcgccgg cgagatgctc ctcctcgaaa cgccgcgcga gatcgccgtg
     4861 gcggtcccac cacgacagcg acttcgccag ctgctcgacg aacagaggat ccagccccag
     4921 cgccttgccc acgtcgcgca cggcgctgcg cgggcgataa ctgatgatgg tcgcggccag
     4981 cgcggcgcgt tcgcgcgtgt acttgcggta gatgtactgg atgacttcct cgcgccgctc
     5041 gtgctcgaag tcgacgtcga tgtcgggcgg ttcgttgcgc tccttcgaga tgaagcgctc
     5101 gaacagaacg gagctgcgat cggggtcgac ctcggtgatg aacaggcagt agcacacggc
     5161 ggaattggcc gccgagccgc gtccctggca caggatgccg ttgccgcgcg cgaactgcac
     5221 gaggtcgtgc acggtcagga aatagtattc gtaggcaagc tcggcgatca ggacgagctc
     5281 cttctcgatc agcgcctgcg cgcgccgcgg cacaccgtcc ggccagcgct gctgtgcgcc
     5341 cgcgagcacc agccggcgca gccaggccgt cggcgtcatg cccggcggca cgacttcctc
     5401 ggggtattcg tagcgcagct ccccgagcgt gaaggaacag cgctgcgcga tgcgcacgct
     5461 ctccgcgagc agcgccgccg ggtagaggcg gcgcagccgc tcgagcgcgc gcaggtggcg
     5521 ctcggcattg gcggcaaggc gcgtgcccag ttgctgcacc ggggtgttga ggcggatcgc
     5581 acacagcgtg tcggcgagcg ccttgcgctg cgcgacgtgc atctcgacgc cgccgcaggc
     5641 gaccatcggc aagccgagcc gggcggcgag ctggtagagc cgcaggtagc gctgctgctc
     5701 gccggcgccg agcagcagtt cgaccccgag ccagagcctc ccggcgaaca gcgtgccgag
     5761 cgcctcgccc tggcgctgca ggggctcgat cgcggcggtg cccggtagcc agatcgccag
     5821 gcagtggcgc acgtttacct cgaagtcgcg caactgcacc gcgtattcac ccttggggct
     5881 gcgccggcgc gccagcgtga tcagtcccga gagctcggca taggcacggc ggtcgggcgc
     5941 cagcagcact agcagcagtt caccgtcgag gcggaactcg gcgccgatga tcaggtgcag
     6001 gccgtgctcg cgcgccgcca cgtgggcctt gaccacgccg gccagcgagc actcgtcggt
     6061 gatcgcgagc gcgcggtagc cctggcgcgc ggcctcggcg accagctcct cggggtgcga
     6121 ggcgccacgc aggaagctga aattgctgat gcagtgcagt tcggcgtagg acatcttcgg
     6181 gtggcggtcg ggcatttact gtatttatat acagtacata aagcctttgc ggctgccaac
     6241 cgctaacatg cgcacctcga taccgcagat ggatcccccg atggccgccc cccgcgccgc
     6301 acccttctgg agcagcaaga ctctcgatca gctgaatcgg gaagaatggg agtcgttgtg
     6361 cgacggctgc gggctgtgct gcctgcagaa gctcgaggac gagtacacac gtcgggtcta
     6421 ctacaccaat gtggcctgca agctgctcga cctggagagc tgccagtgca gcaactacgc
     6481 gcggcgccag gagtacgtgc ccgactgcat cgcgctcacg cccggcgatg ccgatgccta
     6541 ccgctggctc cccgagacct gcgcctaccg cctggtcgcc cagggtgacg agctaccccc
     6601 ctggcaccac ctggtctgcg gcgatcgtgg cgcggtgcat cgtgccggca tctcgcgcgc
     6661 cggccagatg ctgcccgagg ccgccgtgcc cgaggacgcc tgggaagagc acatcatctt
     6721 ccgcatcggc tgaggccggc ccgccatgaa aagaatccgt tccgtgtttg tacaaggaaa
     6781 ctgaatcatg tcgctcgatt tcgacagcgc gcgcctgcgt gatcccaacc ttcgccccga
     6841 gcacgaggag tggcgcacgc agctgcgccg cttcatcgac cgcgagatca tgccccgcgt
     6901 cgatgagtgg gacgaggcct gccgcattcc cgacgagctg tggccgaagg cggccgcgat
     6961 cgggttgctc gggctcggct acccggaagc ctacggcggc accgccgagg gcatcgacat
     7021 ctaccacctg aacatcgttg ccgaggaact cgcgcgcacc agccttggcg gcatcaacag
     7081 cacgctgctg gtgcatggca tcgggctgcc tcccgtggtg aatttcgcca gcgacgcgtt
     7141 gaaggaggcg gtgatcccgc cgatcctgcg cggcgagaag cgcatctcgc tggcgatcac
     7201 cgaaccctcg ggcggctcgg acgtcgccaa catccagacc accgcgcggc gcgacggcga
     7261 gcactacatc gtcaacggcg ccaagacatt catctccggc ggcatgggcg cgaactggtt
     7321 caccaccgcc gtgcgcacgg gcggcgaggg cggacgcggc atctcggtgc tgctcatacc
     7381 ggccgacagc gccggcgtgg cgcgcacgcc gctggcacgc aagcagggct ggtggtgctc
     7441 ggacaccgcg acgatccact tcgacgaggt gcgcgtgccg gcggcgaacc tggtcggtcc
     7501 cgagaacatg ggcttcctgg tgctgatggc caacttcaac cacgagcgca tggcgatggc
     7561 ggtgggcatg gaagccttcg cgcgcgtgtg cctggaggaa gccgtgagct gggcgcgcga
     7621 gcggcgcacc ttcggcaagc gcctcgcgga ccaccaggtc atccgccaca agatcgcgat
     7681 gatgaagcag cgcatcaacg ccacgcaggc ctacatccag atgtgctcgc gccagatcgt
     7741 cgacggcacg gtgcagcccg gcgatatcgc gctgctgaaa gtgcaggcca gcgagaccat
     7801 ggagttctgc gcgcgcgagg cgatgcaggt gctcggtggc gcgggttacc tgcgcggcaa
     7861 ccgcgttgag cgcatctacc gcgaggtgcg cgtgaacgcg atcggcggcg gctcagagga
     7921 gatcatgcgc gatctcgcgg cgcgccagtt cggcttctga gctgtgggtc gggatttatc
     7981 ccgacaaggg ctcggcggcg ccgagcccgc catgccgatc agggcaattg tcggcaagaa
     8041 tgccgaccca caggataaag atcagtcgtc ggcatgaatg ccgacccaca atggcagggc
     8101 aagggggcac cgatgcgttt taccgaagaa caccaggcga ttcgcgccac gacggcgaaa
     8161 ttcatcgatg ccgagatcaa tccccacgcc gacgagtggg aggcagccgg gatattcccg
     8221 gcgcacgagg tgttccgcaa gatgggcgcg ctcgggctgc tcggcatcgc gaagcccgcg
     8281 gaatgcggcg ggctggggct cgattacagc taccagatcg tgttttccga ggaactcggg
     8341 cgcataaagt cgggcggtgt cggcatgggc atcggggtgc agaccgacat ggccacgccg
     8401 gcgctggcca agttcggcag cgac
//
LOCUS       DAORUQ010000138         8409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9327_length_8409_cov_6.030524, whole genome shotgun sequence.
ACCESSION   DAORUQ010000138 DAORUQ010000000
VERSION     DAORUQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9327_length_8409_cov_6.030524"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..840)
                     /gene="ahpC"
                     /locus_tag="PLW13_11680"
     CDS             complement(274..840)
                     /gene="ahpC"
                     /locus_tag="PLW13_11680"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HQY71082.1"
                     /translation="MQSLINTEVKPFKATAFQNGKFETVTEADLKGKWSVVFFYPADF
                     TFVCPTELGDLADNYAEFQRMGVEIYSVSTDTHFTHKAWHDTSETIGKIRYPMIGDPT
                     GAITRNFGVMIEEAGLAERGTFVIDPQGRIQIVEINAGGIGRDATELLRKVKAAQYVA
                     AHPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1150..1920
                     /locus_tag="PLW13_11685"
     CDS             1150..1920
                     /locus_tag="PLW13_11685"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQY71083.1"
                     /translation="MNTVLLEKREGYAIVTLNRPEHMNALSRELRRDFVAVFRDICAD
                     ANIRVVILTGAGAAFCAGFDLKELGSGNSENAAEEVGNELAGAMEAFDGPIIGAINGH
                     AVTGGFEMALACDVLIASENARFADTHARVGILAGWGLSQKLPRMIGMSRAKEISFTG
                     APVFARQACEWGLVNHVVPAAELLPRAVKMAEDMCACVPHILKQYKRLIDDGYSMPYK
                     EALAWETGQSIASARLAAAHLIEARRQGVLAKGRSEKA"
     gene            1917..2684
                     /locus_tag="PLW13_11690"
     CDS             1917..2684
                     /locus_tag="PLW13_11690"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71084.1"
                     /translation="MKGKVALVMGGGAGTGRATAMAFAREGARVVVADINETLAQKTA
                     GLIQAAGGEALAVRADMARAADIQAVMEAARSTWGGLHLVSNNAAAAPPNKALTDITE
                     EEWDRCMSVTLRGVWLCLKHELPLIEASGGGAIVNVASLSGLRGDPHQGAYAAAKGGV
                     LALSKTAATEYARRGVRVNTVCPGGIATRGMEFYLQGMPELRQRTLDVHAMGRLAEPA
                     EIADAVVYLCSDRASFITGHDLVVDGGVLVRSNVIDL"
     gene            2769..4376
                     /locus_tag="PLW13_11695"
     CDS             2769..4376
                     /locus_tag="PLW13_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71085.1"
                     /translation="MNKLEFKDIHDRHIGRSIRMQAEQNGDTRFLVFDRTVYTFHETN
                     EKVNELATGLRRLGIGRGDRVVFYMSSAPEVMFLVLAVNKLGAVWVPVNSDYKGAWLE
                     DTINRGRAKLLVTDTSHAERIAAVAGGLNVERIAVLGDHTCLPGALDFGELYAPSAGE
                     PDLRGMDYGDTSSVLWTSGTTGKSKGVMQSHNVWFEAALGGDVMYDTRPGDVAYNVMP
                     MYNSGAWATSLFRSLFCGITLAIDPAFSVSHFWDRVRFYGATQSFTIGAMHMFLWSAP
                     ARPDDADNPLRELQAVPMPSEIKEPFSERFGVKIMGQGMSQSEAMLILRQDARKRSSW
                     PVGSCGNPVDDIDVMLADDAGNEVAVGEPGELWVRPHRPFLIFNGYFDDPGATANAFS
                     GAWYKTGDLLRRDAGDNYFFVDRKKDAVRYKGRNISTYEVELVARRHPAIADCAAFGI
                     PSEALKSEDEIKLNVILKPGAEATEREIAQFINDNAPYFFVPRYIEFVTELPYTPNQK
                     IQKYKLREAGLGVATWDADKAGFKAAR"
     gene            4465..4731
                     /locus_tag="PLW13_11700"
     CDS             4465..4731
                     /locus_tag="PLW13_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71086.1"
                     /translation="MIRDVLGYAGFAVFGWWLWTGPVHDMRHLPQDEALRFNAQNMAR
                     CMESREFASSLTAPTRADPQRACARKYGLYQSEGRWLAANSPRD"
     gene            4870..5988
                     /locus_tag="PLW13_11705"
     CDS             4870..5988
                     /locus_tag="PLW13_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY71087.1"
                     /translation="MIGFLLGAGPAMADIAVPPDPVDGAYGVLERLGLLDKDASAPPA
                     AVPAPVPASAPVSAATPAPAPQESAEAHAVLQRLGLLPAQERTAPASAAAVSAAPAPA
                     APVAAAPVAASAEPAASARSRFAAGAVGMLERLGMVYKAPPRAPASDAATASATAAAP
                     APVTASGTAIAETEVRKDSVASATTVAAGAAVTTQAVTPPPEPPSYQVLRRLGLLKPL
                     PGAAPGPLAGAGPRVDRVVVEKSKRKLYLVRNGEKFREYPILLGAMPRGPKTQAGDLR
                     TPEGVYTLDWRNPKSRFYKAMHISYPSPSDRERAQEKGVDPGGMVMIHGEHYEPAMRR
                     VLRRNPKDWTEGCIALNNEHIDELWHTVPVGTPIEIKP"
     gene            6012..6314
                     /locus_tag="PLW13_11710"
     CDS             6012..6314
                     /locus_tag="PLW13_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY71088.1"
                     /translation="MTPEPAAMFGIEFPLFAFSHCRDVVVAVSRAGGLGVFGALHHTP
                     EQLREELRWIEAHVDGHPYGIDLVMPEKFVRGCRQMVQEFRESLADALVRIADGGI"
     gene            6331..7272
                     /locus_tag="PLW13_11715"
     CDS             6331..7272
                     /locus_tag="PLW13_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71089.1"
                     /translation="MTYRAHPDDRFHPPASDSPYWTETCWFTFAVPQRRLSGQIYPFF
                     QPNLGVCSAGVYLWDDCGHEPHTIVFGKNFWHLPMPAGDLDDIELANGVSIRCTEPLR
                     RYELRYRDPDADEVAIDLEFTAICAPNYLQGGHYEHAGRYRGTIRIGSEVIPVDSYGM
                     RDRSWSVRSQFGADIHGTGAVRGGYSYATADESHAFHMISMAFVADECIGIHGYYLRE
                     GQFARLAGGKRTVLARDPATRAPVHVLIEARDELGRELRAEGRCLNRIGLHLNPNLFT
                     WNCLTEWCFDGRTAFGEDHDNWTAAGARHFFRRNAGA"
     gene            7276..>8409
                     /locus_tag="PLW13_11720"
     CDS             7276..>8409
                     /locus_tag="PLW13_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HQY71090.1"
                     /translation="MRILHTSDWHLGRQFHHVSLLEDQRHVLEQIVAIVRERAVEVVL
                     VAGDIYDRSVPPAEAVQLLDEVVHRLSAECGVALILIAGNHDGAERLGFGARQLARAG
                     VHIRGPLEPEPEPVILQDAHGPVAFYPLPYAEPATVRSELGLEVQGHEQAMRALTARI
                     RAHNGDSRRSVLIAHCFVDGFAACESERPLSIGGVETVPADCLEGFDYVALGHLHGPQ
                     ARGAKHLRYSGSILKYSFSEENQRKSVTLVDMNAQGACTIEQVALRPRRDLRVLEGAL
                     EDLLEQGRRDPAAQDYLLVRLQDRHAILDPMGKLRTVYPNVLHLERPALAQAGERRAP
                     DRERLREGELPMFRDFFRQMSGEALDEAGEAAVAALLERLHRGE"
BASE COUNT         1353 a         2750 c         2926 g         1380 t
ORIGIN      
        1 tgcgccgagg tggcgacgat ctgctccagc agtacgcgca tctcctctgc cctggcgctg
       61 tcgtcgagcg aggcgacgag ttcgatcggg gccgcgaggc gttccatgta agtcttcaac
      121 tggccggcaa tgttcgcgtc gagcatggta cttctcctgg tgaggactgc ggatgacaag
      181 agaaaggcgc cgcgcctgcg gcacggcggc gcctttgtgt tgtgatcctg aaggggatct
      241 gcgcccggag cggactccgg gcgcaggcgc gactcagatc ttgccgacca ggtcgagcga
      301 cggagcgagc gtcgcctcgc cttccttcca cttcgccggg cagacttcgc cggggtgagc
      361 ggcgacgtac tgcgccgcct tgaccttgcg cagcagttcg gtggcgtcgc ggccgatgcc
      421 gccggcgttg atctccacga tctggatcct gccctgcggg tcgatcacga aggtgccgcg
      481 ctcggccagg cccgcctcct cgatcatcac gccgaagttg cgcgtgatgg cgccggtggg
      541 gtcaccgatc atcgggtagc ggatcttgcc gatggtttcc gaggtgtcgt gccacgcctt
      601 gtgggtgaag tgcgtgtcgg tggacaccga gtagatctcc acgcccatgc gctggaactc
      661 ggcgtagttg tcggccaggt ctccgagctc ggtgggacac acgaaggtga agtcggccgg
      721 gtagaagaac accacggacc acttgccctt cagatcggcc tcggtgacgg tttcgaactt
      781 gccgttctgg aacgcggtgg ccttgaacgg tttgacttcg gtgttgatca gcgattgcat
      841 cgttactgct cctgtgggtt gctgcatcgg gttgtcgttg gcgacgggag caactctagg
      901 ggaacccaat tgatcaatcc aattgaatgt aagggatgag acgatcgctt ttatgaatgg
      961 tttggcgggc cgccgggagt tcccgccgac gcggtgtgat ccgggccgag gcacccggta
     1021 cggtcgcgcg acagcggttc tcgttctgtc gcgtttcgtg cggcaatgtc atcgcatggc
     1081 ttgcggtgtg gcgtcgcgga cgccaatcta cgcacacttg cgacaacccc tttggagcgg
     1141 agccccgcaa tgaacacagt gctgctcgaa aagcgcgagg gttacgccat cgtcaccctc
     1201 aaccgtccgg agcacatgaa cgcgctgtcg cgcgagctgc gccgggattt cgtcgcggtg
     1261 ttccgtgaca tctgtgccga tgcgaacatc cgcgtcgtga ttctcaccgg cgcgggcgcg
     1321 gccttctgtg cgggtttcga cctcaaggag ctcggctcgg gcaactcgga gaatgcggcc
     1381 gaggaggtcg gcaacgagct ggccggggca atggaagcct tcgacggccc gatcatcggc
     1441 gcgatcaacg gccacgcggt caccggcggc ttcgagatgg cgctggcctg cgatgtgctg
     1501 atcgcctccg agaacgcgcg cttcgccgat acgcatgcgc gcgtgggtat cctcgccggc
     1561 tgggggctca gccagaagct gccgcgcatg atcggcatgt cgcgggcgaa ggagatttcg
     1621 ttcaccggtg cacccgtgtt cgcgcgccag gcctgcgagt ggggactggt gaaccacgtg
     1681 gtgcccgccg cggagctgct gccgcgcgcg gtgaaaatgg ccgaggacat gtgtgcctgc
     1741 gtgccgcata tcctcaagca gtacaagcgc ctgatcgacg acggttattc gatgccctac
     1801 aaggaggcgc tggcctggga gaccgggcag tcgatcgcct cggcgaggct cgcggcggca
     1861 cacctgatcg aggctcggcg ccagggcgtg ctggcgaagg gccggagcga aaaggcatga
     1921 agggcaaggt cgcactggtg atgggtggcg gcgcgggcac gggccgtgcc acggcgatgg
     1981 ccttcgcgcg cgagggcgcg cgcgtcgtgg tggccgacat caacgagacc ctggcgcaga
     2041 agaccgccgg actgatccag gcggcgggcg gcgaggctct cgcggtccgt gccgacatgg
     2101 cgagggcggc ggacatacag gcggtgatgg aggccgctcg ctccacctgg ggcgggctgc
     2161 acctggtgtc gaacaatgcc gcggcggcgc cgccgaacaa ggcgctgacc gacatcaccg
     2221 aagaggaatg ggaccgctgc atgagcgtga cgctgcgcgg cgtgtggctg tgcctcaagc
     2281 acgagctgcc gctgatcgag gcttcgggcg gcggcgcgat cgtcaacgtc gcatcgctgt
     2341 cgggcctgcg cggggatccg caccagggcg cctacgccgc ggccaagggc ggcgtgctgg
     2401 cgctgagcaa gacggcggcc acggagtacg cgcgtcgcgg cgtgcgcgtc aacacggtgt
     2461 gtcccggcgg catcgccacc aggggcatgg agttctatct gcagggcatg ccggaactgc
     2521 gccagaggac cctggacgtc cacgccatgg gccggctcgc cgagcccgcg gagatcgccg
     2581 atgcggtggt ctatctctgc tcggatcgcg ccagcttcat caccggccac gacctcgtcg
     2641 tcgatggcgg tgtgctggtg cgctcgaacg tgatcgacct gtagctgcgc atcgatgcgg
     2701 ataggggcgg gggaatgtcc ggataaatcc gggcctacgg tatgaatagg aaggaagctc
     2761 gcaacgcgat gaacaagctc gaattcaagg acatccacga ccgccacatc ggccgctcga
     2821 tccgcatgca ggccgagcag aacggcgaca cccgcttcct ggtgttcgac cgcaccgtct
     2881 acacgttcca cgagaccaac gaaaaggtga acgagctcgc cacgggcctg cgccggctcg
     2941 gcatcgggcg cggcgaccgc gtggtgttct acatgtcgag cgccccggaa gtgatgttcc
     3001 tggtgctggc ggtcaacaag ctcggagcgg tctgggtgcc cgtcaacagc gactacaagg
     3061 gcgcgtggct cgaggacacc atcaaccgcg gccgcgcgaa acttctggtc accgacacca
     3121 gtcatgccga gcgcatcgcg gcggtcgcgg gcgggctgaa cgtggagcgc atcgcggtgc
     3181 tgggcgatca cacctgcctg cccggcgcgc tggacttcgg ggagctctac gcgccttcgg
     3241 ccggcgagcc ggacctcagg ggcatggact acggcgatac cagctcggtg ctgtggacct
     3301 caggcaccac gggcaagtcc aagggcgtga tgcagagcca caacgtgtgg ttcgaggccg
     3361 cgctcggcgg cgacgtgatg tacgacacgc gccccggcga cgtcgcctac aacgtgatgc
     3421 cgatgtacaa ctccggcgcc tgggcgacct cgctgtttcg cagcctgttc tgcggcatca
     3481 cgctggcgat cgatcccgcg ttctccgtga gccatttctg ggaccgtgtg cgcttctacg
     3541 gcgccacgca gtcgttcacg atcggcgcga tgcacatgtt cctgtggagc gctccggcgc
     3601 gccccgatga cgccgacaac ccgctgcgcg aactgcaggc cgtgccgatg cccagcgaga
     3661 tcaaggagcc cttcagcgag cgcttcggcg tgaagatcat ggggcagggc atgtcgcaga
     3721 gcgaggccat gctgatcttg cgccaggacg cgcgcaaacg ctcgagttgg cccgtcggca
     3781 gctgcggcaa cccggtcgac gacatcgacg tcatgcttgc cgacgatgcc ggcaacgagg
     3841 tggcggtggg cgagccgggc gagctgtggg tgcgtccgca ccgcccgttc ctcatcttca
     3901 acggttattt cgacgacccc ggcgccacgg cgaacgcctt ttccggcgcg tggtacaaga
     3961 ccggtgacct gctgcgccgg gacgccgggg acaactactt cttcgtcgac cgcaagaagg
     4021 acgcggtgcg ctacaagggc cgcaatatct cgacctacga ggtggagctg gtggcgcgcc
     4081 gccatcccgc cattgccgac tgcgcggcct tcggcattcc ttccgaagcg ctgaagagcg
     4141 aggacgagat caagctgaac gtgatcctga agcccggcgc cgaggcgacc gagcgcgaga
     4201 tcgcgcagtt catcaacgac aatgcgccat acttcttcgt gccgcgctac atcgagttcg
     4261 tcaccgagct gccttacacg ccgaaccaga agatccagaa gtacaagctg cgcgaggccg
     4321 gcctgggcgt ggcgacctgg gacgcggaca aggcgggctt caaggccgcg cgttgatcgc
     4381 gagcgtgatt tgcgaccggc atcacgccgt gtgtcgcgtg cagcagccat acttgcgccg
     4441 gttcagctga acaggagcgc gcgcatgatc cgggacgtgc tcggatacgc gggttttgcg
     4501 gtcttcgggt ggtggttgtg gactggcccg gtccatgaca tgcgccacct gccgcaggac
     4561 gaggcgctca ggttcaacgc acagaacatg gcgcgatgca tggagtcgag ggagttcgcc
     4621 tcgagtctca ctgcgccgac gcgtgccgat ccccagcgcg cctgtgcccg caagtacggc
     4681 ctctaccaga gcgagggccg ctggctcgcg gcgaattcgc cgcgcgactg agcggttttc
     4741 ggtctttcgc atcgcggact gctggcactg gcagtctgcg cgtctataat tgcccgcagt
     4801 cgaagaattt ctggctttgg agggcgacct ggcgtgtctg gatccgtgct gcggcattgc
     4861 gtgatatcga tgatcgggtt tttgctgggt gccggtccgg ccatggccga tatcgcggtg
     4921 ccgccggatc cggtcgatgg ggcctacggc gtgctggagc gcctcggtct gctggacaag
     4981 gacgcctcgg ccccgcccgc ggcggtgccg gcacccgtgc ccgcatcggc gccggtttcc
     5041 gcggccacac cggcgcccgc gccgcaggag tccgccgagg cccatgcggt gctgcaacgc
     5101 ctgggactgc tgccagcgca ggagcgaacc gcgccggcat ccgctgccgc cgtatccgcc
     5161 gcaccggcac cggctgcacc ggtagctgcc gcgcccgtgg cggcatccgc cgaacctgct
     5221 gcctccgcgc gctcgcgttt cgcggccggg gccgtcggca tgctcgagcg cctcggaatg
     5281 gtgtacaagg cgccgccgcg cgcgcctgca tctgatgccg cgacggcctc cgccactgcg
     5341 gctgcacccg cgccggtgac ggcgagcggc acggctatag ccgagaccga ggtacgcaag
     5401 gactcggtcg cgagcgcgac aacagtggcc gctggcgccg cggttacgac ccaagccgtg
     5461 acgccgccac ccgagccgcc ttcctaccag gtgctgcgcc ggctcggact gttgaagccg
     5521 ctcccgggtg ctgccccggg tcccctggcc ggtgccgggc cccgcgtcga tcgcgtggtc
     5581 gtcgagaaga gcaagcgcaa gctctacctg gtgcgcaacg gcgagaaatt ccgcgaatac
     5641 ccgatcctgc tcggcgccat gccgcgcggt ccgaaaacgc aggccggcga tctgcgcacg
     5701 cccgagggcg tgtacacgct ggactggcgc aacccgaaga gccgcttcta caaggccatg
     5761 cacatttcct acccgagccc gagcgatcgc gaacgggccc aggagaaagg agtggatccg
     5821 ggcggcatgg tgatgatcca cggcgagcat tacgagcctg ccatgcgccg tgtcctgcgc
     5881 cgcaatccca aggactggac cgagggctgc atcgcgctca acaacgagca catcgacgag
     5941 ctctggcaca cggtgccggt tggcacgccg atcgagatca agccctgatc gcgcgcgcat
     6001 tgatgcttca catgacgccg gaacctgccg cgatgttcgg catcgaattc ccgctgttcg
     6061 ccttcagcca ctgtcgcgac gtggtggtgg ccgtgagcag ggcgggcggt ctcggggtat
     6121 tcggtgcgct gcaccacacg ccggaacaat tgcgcgagga actgcgctgg atcgaggcgc
     6181 acgtggacgg gcatccttac ggcatcgacc tggtgatgcc ggagaaattc gtgcgcggct
     6241 gccgccagat ggtgcaggaa ttccgcgaat cccttgccga cgccctcgtc cggatagcgg
     6301 acggagggat ctgattgacg ggggcccaga gtgacttacc gcgcgcatcc cgacgaccgc
     6361 ttccacccgc ccgcgagcga cagcccgtac tggacagaaa cctgctggtt caccttcgcg
     6421 gtgccgcagc gccggctgtc gggacagatc taccccttct tccagcccaa tctgggcgtg
     6481 tgctcggcgg gcgtctacct ctgggatgac tgcgggcacg aaccgcacac gatcgtgttc
     6541 ggaaagaatt tctggcacct gccgatgccg gccggggacc tggacgacat cgagctcgcc
     6601 aacggcgtca gcattcgttg caccgagcct ctgcgccgct acgaactgag gtaccgcgat
     6661 cccgatgccg acgaagtggc gatcgacctc gagttcaccg cgatctgcgc gcccaattac
     6721 ctgcagggcg ggcattacga gcacgcgggc cgctaccgcg gcaccatccg catcggcagt
     6781 gaagtcatcc ccgtcgacag ctacggcatg cgtgaccgct cgtggagcgt gcgctcgcag
     6841 ttcggcgccg acatccacgg cacgggggcc gtgcgcgggg gctacagcta cgcgacggcc
     6901 gacgagagtc acgcgttcca catgatcagc atggccttcg tggccgacga gtgcatcggc
     6961 atccacggct attacctgcg cgaggggcag ttcgcgcgcc tggccggcgg caagcgcacg
     7021 gtgctggcgc gcgacccggc gacgcgcgcg ccggttcacg tgctgatcga ggcgcgcgac
     7081 gaactcgggc gcgagttgcg cgccgagggg cgctgcctga accggatcgg gctgcacctg
     7141 aatcccaacc tcttcacctg gaactgcctc accgaatggt gcttcgacgg gcgcaccgcc
     7201 tttggcgagg atcacgacaa ctggaccgcc gccggtgcac gccacttctt ccgccgcaac
     7261 gccggcgcct gagcgatgcg gatcctgcat acctcggact ggcacctggg gcgacagttt
     7321 caccacgtct cgctgctgga agaccagcgt cacgtgctgg aacagatcgt ggcgatcgtg
     7381 cgcgagcgcg ccgtcgaggt ggtgctggtt gccggtgaca tctacgaccg ttcggtgccg
     7441 cccgccgagg cggtgcaatt gctcgacgag gtggtccatc gcctgagcgc ggagtgcggc
     7501 gtggcgctga tcctgatcgc cggcaaccac gacggcgccg agcgtctggg cttcggggcg
     7561 cggcaactgg cgcgcgcggg cgtgcacatc aggggcccgc tcgagcccga gcccgagccc
     7621 gtgatactgc aggacgcaca cggtcccgtg gcgttctatc ccctgcctta cgctgagccg
     7681 gcaacggtgc gcagcgagct cggcctcgag gtgcagggcc acgagcaggc gatgcgcgcg
     7741 ctcaccgcgc gcatccgcgc gcacaacggc gacagccggc gcagcgtgct gatcgcccat
     7801 tgtttcgtcg acggcttcgc cgcctgcgag tccgagcgcc cgctgtcgat cggcggggtg
     7861 gagacggtgc cagcggactg cctcgagggc ttcgattacg tcgcgctggg ccacctgcat
     7921 ggcccgcagg cgcgcggcgc gaagcacctg cgctactcgg gctcgatact caagtactcg
     7981 ttctcggagg agaaccagcg caaatcggtg acgctggtcg acatgaacgc gcagggcgcc
     8041 tgcaccatcg agcaggttgc gttgcgcccg cgtcgcgatc tgcgcgtgct ggagggcgcg
     8101 ctcgaggatt tgctcgagca gggccgccgt gaccccgccg cgcaggacta cctgctggtg
     8161 cggttgcagg accggcacgc gatcctcgat ccgatgggca agctgcgcac ggtctatccg
     8221 aacgtgctgc acctcgagcg tccggcgctg gcgcaggccg gcgagcgccg cgcgccggac
     8281 cgcgagcgcc tgcgcgaggg cgaattgccg atgttccgcg atttcttccg ccagatgagc
     8341 ggcgaggccc tggatgaggc gggcgaggcg gccgttgccg cgttgctgga acgccttcac
     8401 cgcggcgag
//
LOCUS       DAORUQ010000139         8374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9382_length_8374_cov_6.019594, whole genome shotgun sequence.
ACCESSION   DAORUQ010000139 DAORUQ010000000
VERSION     DAORUQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_8374_cov_6.019594"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLW13_11725"
     CDS             complement(<1..482)
                     /locus_tag="PLW13_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY71091.1"
                     /translation="MEQALHSNPIPQAGTAAVQPAWTSAIERHRSGDTEGAIVLYQRH
                     LKRNPQDAPGWMNLGAALRRTGRADAALVCYQRALRLRPDDAAVWSNLGNLWKQFEEH
                     EQSLYCHRRALQLDPQRMQLQLNCAVSLREAGHFTAAEDIIEQCLAREPQRPDLLYER
                     A"
     gene            complement(485..1870)
                     /locus_tag="PLW13_11730"
     CDS             complement(485..1870)
                     /locus_tag="PLW13_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQY71092.1"
                     /translation="MIKRIPPMSPREPRPDDARFRPAAIALRDGEPAMPGRWLLRAVF
                     LLLAVLLAWAMIGRVDVVAVATGRVIPDGRSKVLQSLDGGIVRAIHVREGEAVQEGQV
                     LLELDGAELLAELARVRGELQAERLALGAALALWRLLESGDADGTGPVLAAALREAGS
                     APDAEQFALQAWLLESRLREQARRHDALAQRFDARMAAQRAIAANLVRLEHGVPILAE
                     RAASARELHERHLLARQQWQQQDLERVAMEQELQGERERGAALAREIAEIAAEREAFE
                     AGSLREALMAVEARRRGADALARDRERLEERAARLELRSPATGTVQQLAVRAVGAVLE
                     PAEPVLVVVPRDAALEVEAFVLNRDIGFVGSGQQAAVKVDTFEFTRYGLLPARVRSVS
                     AEAVEHPQFGAVYPARVALERDWIAVGEGRATLVPGMAVQVEIRTGTRKIIDYFLSPL
                     GRVVHDSIRER"
     gene            complement(1881..4040)
                     /locus_tag="PLW13_11735"
     CDS             complement(1881..4040)
                     /locus_tag="PLW13_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004665125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HQY71093.1"
                     /translation="MDKTPSAFLPDSGPPPTDSGLAALVLVARLHGLATDPDTLRHRH
                     GSGDGPLDAAALVRAARDTGLRARLRSATDDGLDTLPLPALAETRAGGWLVLARVSAE
                     RVLVQETGATQPRIMARAEFLATWSGRLLLVARRGAGAQCTRFGIGWFLRAARPYRRI
                     LAEVLLGSAVLQVLGLATPVFFQVVVDKVLVHKVLGTLQVLVAGFVVVSVFEVLLGGL
                     RTWLLAHTTNRIDVTLGARLFAHLLALPLRYFEVRRVGEIAARVRELETLRAFVTGSA
                     LTLLIDLGFTVIAFALMYHYSPLLCAVVALSLPLYLLLSLAAAPALRAQVEEKFRLGA
                     ENQSFLVESLAGMHTLKAMALEPPMQRRWEEQLAGYVSAAYRATLTANWSAQVANLVS
                     RLVSAGLLWFGAGAVIAGTLSVGELIAFNMLAMRSGAPVLRLFQLWQDFQQARVSLAR
                     LGDILNSPGETGVHASRAALPALAGAIRFERVSFRYEPERAHALCELDLAIAPGEVIG
                     IVGRSGSGKSTLARLVQRLYVPEQGRILIDGCDIALAAPDALRRQIGVVPQECFLFNA
                     SVRENIALGDPGMPLERVMNAARLAGAHEFIAELPYGYDTVLGEHAHNLSGGQRQRLA
                     IARALVRDPRILVFDEATSALDYESESVVRANLEAICAGRTVIIIAHRLSALARCERI
                     LVLDRGRLVEQGTPAALLAAGGLFARLHALQSPARAA"
     gene            4229..5815
                     /locus_tag="PLW13_11740"
     CDS             4229..5815
                     /locus_tag="PLW13_11740"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HQY71094.1"
                     /translation="MQNTVTNLFGDSQQPVAAAHRARVVAITSGKGGVGKTSITANIA
                     VALALAGKRVCIVDADTGLANINIVMGLTPRHTLEDFLDGALPIEAILMEGPRGVRVV
                     PGASGIVEYADLGRHHQQSLLNGMQRLEEQFDYLLIDTAAGIADSVIHFVLAAERALL
                     VISPDPTSLTDAFALTRILKRNNYTGRLHALVNMADSVESARKVYQRFAQAVHKYMQV
                     DIDWFGHVSADRAVVTSVRLQHPVILVQPDAPASRCFHALAQGLEQACSGEQGPGLAE
                     HLRSLQPETAVDPASAAIDMIRSAQPTRPVAREANLGELHRQFIDCIQESKGSAEELV
                     AAIKPIIDAYVARFHSFPLDMREAIYRYLEMGDFPDHEIRNQVMMLEQLYEKRYQRPL
                     MDKEDSLFRLLNQVRDSEADFASTIARLQQSYERQYRPMPQEALAALTERLRQPEVEE
                     QDVAQCIEALHSLYEDRFARRYVTEDRALRERVGALATELASREAARRDTLAIIAGEI
                     EQSLQHDERLQAVLRELAAP"
     gene            complement(5925..8312)
                     /gene="lon"
                     /locus_tag="PLW13_11745"
     CDS             complement(5925..8312)
                     /gene="lon"
                     /locus_tag="PLW13_11745"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQY71095.1"
                     /translation="MTDQPTTDKEAVPGVPGSTSLVLPNQALPEILYLMPVTTRPFMP
                     AQIQPMMADASAWGDTVQRVAQTEHKVMGLFFVDTDDVASIKPADIPLVGCAVRLLNA
                     AGDGKRVQFVAQGTGRARIKRWINRTPPYVVQVEYPKDELERNDETRAWAMALVGAIK
                     ELVPLNPLYNEDLKHYLMRFSPEDPSPLTDFAATLTSAGGRELQEVLETLPVVERMRM
                     VLPLLKKEIEVARLQDRISTQVNETVSERQREFFLREQLKVIKQELGLSKDDRSADVE
                     RFEARLAGLTVPEHAMERIRDELDKLAILETGSPEYGVTRNYLDWATSVPWGVHSEDK
                     LDLAHARGVLEREHDGLEDVKERILEFIAVGAYKGAISGSIILLVGPPGVGKTSIGKS
                     IAHALGREFYRFSLGGMRDEAEIKGHRRTYIGAMPGKFVQALKQVKVANPVIMLDEID
                     KVGASYQGDPASALLEALDPEQNAEFLDHYLDLRVDLSKVLFVCTANQLDTIPAPLLD
                     RMEVIRLAGYIAEEKLAIAKHHLWPRLLERAGVKPVRLRISDAALRHVIDGYAREAGV
                     RNLEKRLASLVRKSVVKLLGNPGAKLRIGREEVEQMLGAPVFAKEKQLRGVGVVTGLA
                     WTSMGGATLPVEATRVHSLNRGFKLTGKLGEVMKESAEIAYSYVMSHLAHYDADPKFF
                     DESLVHLHVPEGATPKDGPSAGVTMATALLSLVRKEKLARPLAMTGELTLTGQVLPVG
                     GIREKVIAARRVGIRELVLPEANRKDFDELPEYLREDFEVHFASRYDDVFRAVFA"
BASE COUNT         1276 a         3042 c         2845 g         1211 t
ORIGIN      
        1 gcgcgctcgt acagcaggtc cgggcgctgc ggctcgcgcg ccaggcactg ctcgatgatg
       61 tcctcggcgg ccgtgaaatg cccggcctcg cgcagcgaca cggcgcagtt gagctgcagc
      121 tgcatgcgct gcggatccag ttgcagcgcg cggcggtggc agtagagcga ctgctcgtgc
      181 tcctcgaact gcttccacag gttgccgagg ttgctccaca ccgcggcatc gtcgggccgc
      241 aaccgcagcg cgcgctggta gcacaccagc gccgcgtcgg cgcgcccggt gcgccgcagc
      301 gccgcgccga ggttcatcca gcccggcgcg tcctgcgggt tgcgcttgag atggcgctgg
      361 tagagcacga tcgcgccctc ggtgtcgccg ctgcggtggc gctcgatggc cgatgtccac
      421 gccggctgca ccgcggcggt gccggcttgg ggtatggggt tcgagtgcaa tgcctgttcc
      481 atggctaacg ctccctgatg ctgtcgtgaa ccaccctgcc gagcggcgac aggaaataat
      541 cgatgatctt ccgggtgccg gtcctgatct ccacctgcac ggccatgccg ggcaccagcg
      601 tggcgcgccc ctcgccgacc gcgatccagt ctcgctcgag cgcgacacgc gcggggtaca
      661 ccgcgccgaa ctgcggatgc tcgacggcct cggccgacac cgagcgcacg cgcgccggca
      721 gcaggccgta gcgcgtgaac tcgaaggtat cgaccttcac cgcggcctgc tgcccgctgc
      781 cgacgaagcc gatgtcgcga ttgagcacga aggcctcgac ctccagcgcg gcatcgcgcg
      841 gcacgacgac cagcaccggc tcggcgggct cgagcaccgc accgaccgcg cgcaccgcga
      901 gctgctgcac tgtgccggtc gccggtgagc gcagctcgag acgggcggcg cgctcctcga
      961 ggcgctcgcg atcgcgcgcc agcgcatcgg cgccgcgacg gcgcgcctcg accgccatca
     1021 gcgcctcgcg caggctgccc gcctcgaagg cctcgcgctc ggccgcgatc tcggcgatct
     1081 cgcgcgcgag cgctgcgcca cgctcgcgct cgccctgcag ttcctgctcc atcgccacgc
     1141 gctccagatc ctgctgctgc cactgctggc gcgcgagcag atggcgctcg tgcaactcgc
     1201 gcgccgaggc cgcgcgttcc gcgagaatcg gcacgccgtg ctcgagcctg acgaggttgg
     1261 cggctatcgc gcgctgcgcc gccatgcgcg catcgaagcg ctgcgccagc gcatcgtgcc
     1321 ggcgtgcctg ctcgcgcagc cgcgattcga gcagccacgc ctgcagggcg aactgctcgg
     1381 cgtcgggcgc gctgccggcc tcgcgcaagg ccgcggccag cacggggcct gtcccgtcgg
     1441 cgtcgcctga ctccagcagc cgccacagcg ccagcgcggc tcccagcgcg aggcgctcgg
     1501 cctgcagttc gccgcgcacg cgggcgagct ccgccaacag ctccgcgccg tcgagctcga
     1561 gcagcacctg cccttcctgc accgcctcgc cctcgcgcac gtggatcgcg cgcacgatgc
     1621 cgccgtccag cgactgcagc accttgctgc gcccgtcggg aatcacgcgc cccgttgcga
     1681 ccgccaccac gtcgacgcgc ccgatcatcg cccacgccag cagcaccgcg agcagcagga
     1741 acaccgcgcg cagcagccag cgaccgggca tcgcgggctc gccgtcgcgc agcgcaatcg
     1801 ccgccggccg gaagcgcgca tcgtcaggcc gcggttcgcg cggcgacata gggggaattc
     1861 gcttgatcat gcgcgcctcc tcaggccgcc cgcgccggcg actgcagcgc gtgcaggcgc
     1921 gcgaacaacc caccggcggc gagcagcgcg gccggcgtgc cctgctccac caaccgtccg
     1981 cgatccagca ccaggatgcg ctcgcagcgc gccagcgcgc tcaagcggtg cgcgatgatg
     2041 atcacggtgc gcccggcgca gatcgcctcg aggttggcgc gcaccacgct ctccgattcg
     2101 tagtcgagcg cgctggtcgc ctcgtcgaag acgaggatgc gcgggtcgcg caccagcgcc
     2161 cgcgcgattg cgagacgctg gcgctgcccg ccggagaggt tgtgcgcgtg ctcgcccagc
     2221 acggtgtcgt agccgtaggg cagctcggcg atgaactcgt gcgccccggc gaggcgcgcc
     2281 gcgttcatca cgcgctccag cggcatgccc gggtcgccca gcgcgatgtt ctcgcgcacg
     2341 ctggcgttga acaggaagca ctcctgcggc accacgccga tctggcggcg cagcgcatcg
     2401 ggcgcggcca gcgcgatgtc gcagccgtct atgaggatgc gtccctgctc cggcacatag
     2461 aggcgctgca ccagccgcgc cagcgtgctc ttgcccgagc ccgagcgccc gacgatgccg
     2521 atcacctcgc ccggcgcgat ggccaggtcg agttcgcaga gcgcgtgcgc gcgctcgggc
     2581 tcgtagcgga acgacacgcg ctcgaaacgg atcgcgccgg cgagcgccgg cagtgcggcg
     2641 cgtgacgcat gcacgccggt ctcgccgggg ctgttcagga tgtcgccgag gcgcgccagc
     2701 gagacgcgcg cctgctggaa gtcctgccac agctggaaca gccgcagcac gggcgcgccg
     2761 ctgcgcatcg cgagcatgtt gaaggcgatc aattcgccca cgctcagcgt gccggcgatc
     2821 accgcgccgg cgccgaacca cagcagcccc gcgctcacca ggcggctgac caggttcgcg
     2881 acctgcgccg accagttcgc cgtcagcgtg gcgcggtagg ccgcgctcac gtagcccgcg
     2941 agctgctcct cccagcgtcg ctgcatcggc ggttccagcg ccatcgcctt cagcgtgtgc
     3001 atgccggcca gcgattcgac caggaaggac tggttctcgg cgccgaggcg gaacttctct
     3061 tccacctgcg cgcgcagcgc cggcgcggct gcgagcgaga gcagcaggta gagcggcagc
     3121 gacagcgcga ccaccgcgca cagcagcggg ctgtagtggt acatcagggc gaaggcgatc
     3181 acggtgaagc caagatcgat cagcaaggtg agcgccgagc cggtgacgaa ggcgcgcagc
     3241 gtctccagct cgcgcacgcg cgcggcgatc tccccgacgc ggcgcacctc gaagtagcgc
     3301 agcggcagcg ccagcaggtg cgcgaacagc cgcgcgccga gcgtgacgtc gatgcggttg
     3361 gtggtgtgcg cgagcagcca ggtgcgaagc cccccgagca gcacctcgaa caccgacacc
     3421 accacgaagc ccgcgaccag cacctgtagc gtgccgagca ccttgtgcac cagcaccttg
     3481 tcgaccacga cctggaagaa caccggcgtg gcgagcccca gcacctgcag caccgccgag
     3541 cccagcagca cctcggcgag gatgcgccgg tagggccgcg cggcgcgcag gaaccagccg
     3601 ataccgaaac gcgtgcactg cgccccggcg ccgcgccgcg cgaccaacag cagccggccg
     3661 ctccaggtgg cgaggaactc ggcgcgcgcc atgatgcgcg gctgagtcgc gccggtctcc
     3721 tgcaccagca cacgctcggc ggagacccgc gccagcacca gccagccgcc cgcgcgcgtc
     3781 tcggcgagcg cgggcagcgg cagcgtgtcg agcccgtcgt ccgtggcgct gcgcaggcgc
     3841 gcgcgcagcc cggtgtcgcg cgccgcgcgc accagcgccg cggcatccag cggcccgtcg
     3901 ccgctgccgt ggcgatgcct cagggtgtcg gggtcggtgg cgagaccgtg cagccgcgcg
     3961 acgagcacga gcgccgcgag gccgctgtcg gtcggcggag ggccggaatc cggaaggaaa
     4021 gcagaagggg tcttgtccat gttacgtccc tgtaacggtc aagcacggcc ccacgctacg
     4081 cccgtccccg gcggcgtgca agcccccgtt ggcaagctgg ccgcaaatca ccttcgaagt
     4141 ccgtaggtcg ggtttcaacc cgacatgggt tctgtcggca tgaatgccga cctacaatgc
     4201 atggacaatt ccggcgcgac gggaccccat gcagaacacg gtgaccaatc tcttcggcga
     4261 ttcgcagcaa ccggtcgcgg cagcgcaccg cgcgcgcgtg gtcgcgatca ccagcggcaa
     4321 gggcggcgtc ggcaagacca gcatcacggc caacatcgcg gtggcgctgg cgctcgcggg
     4381 caagcgggtg tgcatcgtcg atgccgacac gggcctggcg aacatcaaca tcgtgatggg
     4441 cctgacgccg cggcacacgc tggaggattt cctcgacggc gcgctgccga tcgaggcgat
     4501 cctgatggaa ggcccgcgcg gcgtgcgcgt ggtgcccggg gcatccggga tcgtggaata
     4561 cgccgacctc ggccgccacc accagcagtc cttgctcaac ggcatgcagc ggctggagga
     4621 gcagttcgac tacctgctga tcgacaccgc ggccggcatc gccgactcgg tgatccactt
     4681 cgtgctcgcg gccgagcgcg cgctgctggt gatatccccg gatcccacct cgctcaccga
     4741 cgccttcgcg ctcacgcgca tcctcaagcg caacaactac acgggccggc tgcacgcgct
     4801 ggtgaacatg gccgacagcg tggaatccgc gcgcaaggtc taccagcgtt tcgctcaggc
     4861 ggtgcacaag tacatgcagg tcgacatcga ctggttcggc cacgtcagcg ccgaccgcgc
     4921 cgtggtcacc tcggtgcggc tgcagcaccc ggtgatcctg gtgcagcccg acgcgccggc
     4981 cagccgctgc ttccacgcgc tggcgcaggg gctcgagcag gcctgttccg gcgagcaggg
     5041 accggggctc gccgagcacc tgcgctcatt gcagcccgag accgcggtcg atccggcctc
     5101 ggccgcgatc gacatgatcc gcagcgcgca gccgacgcgc cccgtggcgc gcgaggcgaa
     5161 cctcggcgag ctgcaccgcc agttcatcga ctgcatccag gagagcaagg gcagcgccga
     5221 ggagctggtg gcggcgatca agccgatcat cgacgcctac gtggcgcgct ttcattcctt
     5281 tccgctcgac atgcgcgagg cgatctaccg ctacctggaa atgggggact tccccgacca
     5341 cgagatccgc aaccaggtga tgatgctcga gcagctctac gagaagcgct accagcgtcc
     5401 gctgatggac aaggaagaca gcctgttccg gctgctcaac caggtacgcg attccgaagc
     5461 cgatttcgcc tcgaccatcg cgcgtctgca gcagagctac gagcgccagt accgcccgat
     5521 gccgcaggag gccctcgccg cgctcaccga gcggctgcgc cagccggaag tcgaggagca
     5581 ggacgtggcg cagtgcatcg aggcgctgca ctcgctctac gaggaccgct tcgcgcggcg
     5641 ctacgtcaca gaggaccgcg cgctgcgcga gcgcgtgggc gcgctcgcca ccgagctggc
     5701 atcacgcgaa gccgcgcgcc gcgacaccct cgcgatcatc gccggcgaga tcgagcaatc
     5761 actgcagcac gacgaacggc tgcaggcggt gttgcgggag ctcgctgcgc cgtagggttg
     5821 atatggtcgc gggcatggcc cgctcctaca cccatcccgg cgccgtcgtg tattcgggct
     5881 tgtaggaacg ggccatgccc gcgaccacaa ctcctcccgg taacctacgc gaagaccgcg
     5941 cggaacacat cgtcgtagcg cgaggcgaaa tgcacctcga agtcctcgcg caggtactcg
     6001 ggcaactcgt cgaagtcttt gcggttcgcc tcgggcagca ccagctcgcg gatgccgacg
     6061 cggcgcgccg cgatcacctt ctcgcggatt ccgcccacgg gcagcacctg cccggtgagc
     6121 gtcagctcgc cggtcatcgc gagcgggcgt gcgagctttt ccttgcgcac cagcgacaac
     6181 agcgcggtgg ccatggtgac gccggcgctg gggccgtcct tgggcgtggc gccctcgggc
     6241 acgtgcaggt gcaccagcga ctcgtcgaag aacttcggat cggcgtcgta gtgcgccagg
     6301 tgcgacatca cgtagctgta ggcgatctcc gccgattcct tcatcacctc gccgagcttg
     6361 ccggtgagct tgaagccacg gttcagcgag tgcacgcgcg tggcctccac cgggagcgtg
     6421 gcgccgccca tcgaggtcca cgccagcccg gtgaccacgc ccacgccgcg caactgtttc
     6481 tccttcgcaa aaaccggcgc gccgagcatc tgctcgacct cctcgcgccc gatgcgcagc
     6541 ttcgcgcccg ggttcccgag cagcttcacc acgctcttgc gcaccaggct cgcgaggcgc
     6601 ttctcgaggt tgcgcacgcc ggcctcgcgc gcgtagccgt cgatcacgtg gcgcagcgcg
     6661 gcatcgctga tgcgcagccg caccggcttc acgccggcgc gctccagcag ccgcggccac
     6721 aggtggtgct tggcgatcgc gagtttttcc tcggcgatgt aaccggcgag gcggatcact
     6781 tccatgcgat ccagcagcgg cgcgggaatg gtgtcgagct ggttcgcggt gcacacgaac
     6841 aataccttcg agagatccac gcgcaggtcg agatagtgat cgaggaactc ggcgttctgc
     6901 tcggggtcga gcgcttcgag cagcgccgat gccgggtcgc cctggtagga ggcgccgacc
     6961 ttgtcgatct cgtcgagcat gatcacgggg ttggcgacct tcacctgctt caacgcctgc
     7021 acgaacttgc cgggcatggc accgatgtag gtgcgccggt ggcccttgat ctcggcctcg
     7081 tcgcgcatgc cgcccaggct gaagcggtag aactcgcgcc ccagcgcatg cgcgatggat
     7141 ttgccgatcg aggtcttgcc cacgcccggc gggccgacca gcaggatgat cgagccgctg
     7201 atcgcgccct tgtacgcgcc gaccgcgatg aactcgagga tgcgctcctt cacgtcctcc
     7261 agcccgtcgt gctcgcgctc cagcacgccg cgcgcgtgcg ccagatcgag cttgtcctcg
     7321 gagtgcacgc cccacggcac cgaggtggcc cagtcgaggt agttgcgcgt gacgccgtac
     7381 tcgggcgagc cggtctcgag gatcgcgagc ttgtcgagct cgtcgcggat gcgctccatc
     7441 gcgtgctcgg gcaccgtgag gccggcgagg cgcgcctcga agcgctcgac atcggcgctg
     7501 cgatcgtcct tcgacaggcc gagctcctgc ttgatgacct tcagctgctc gcgcaggaag
     7561 aactcgcgct ggcgctcgct gaccgtctcg ttcacctgcg tgctgatgcg gtcctgcagc
     7621 cgcgcgacct cgatctcctt cttcagcagc ggcagcacca tgcgcatgcg ctccaccacc
     7681 ggcagcgttt ccagcacctc ctgcagctcg cgcccgccgg cgctggtgag cgtggcggcg
     7741 aagtccgtga gcggcgaggg gtcctcgggg ctgaagcgca tcaggtagtg tttcaggtcc
     7801 tcgttgtaga gcgggttgag cggcaccagc tccttgatcg cgcccaccag cgccatcgcc
     7861 caggcgcgcg tctcgtcgtt gcgctccagc tcgtccttcg gatattcgac ctgcaccacg
     7921 tagggcggcg tgcggttgat ccagcgcttg atgcgcgcgc gccccgtgcc ctgcgcgacg
     7981 aactgcacgc gcttgccgtc acctgcggca ttgagcaggc gcacggcgca gccgaccagc
     8041 gggatgtccg cgggcttgat cgaggcgacg tcgtcggtgt cgacgaagaa cagccccatc
     8101 accttgtgct cggtttgcgc gacgcgctgc accgtgtcgc cccaggcgct ggcgtcggcc
     8161 atcatcggct ggatctgcgc cggcatgaac gggcgcgtcg tgaccggcat gaggtagagg
     8221 atttcgggca gtgcctggtt cggcagcacc aggctggtgc tgccggggac gccggggacg
     8281 gcttccttgt cggtcgtcgg ctgatcggtc atcgtgaatc tccgttggtc tcatccggca
     8341 tatggggcct ggcgtacact aaatcaacca cccg
//
LOCUS       DAORUQ010000140         8259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9591_length_8259_cov_5.778401, whole genome shotgun sequence.
ACCESSION   DAORUQ010000140 DAORUQ010000000
VERSION     DAORUQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_8259_cov_5.778401"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PLW13_11750"
     CDS             complement(<1..1622)
                     /locus_tag="PLW13_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HQY71096.1"
                     /translation="MSASEIQHAIRHGLYDPSREHDACGVGFVAHIKGQRSHSIIEQG
                     LKILENLDHRGAVGADKLMGDGAGVLIQIPDELYREEMAKQGLQLPPPGEYGVGMVFL
                     PKEHASRLACEQELERAVRAEGQVLLGWRDVPVDREMPMSPTVRAKEPVIRQIFIGRG
                     QDIIVPDALERKLYVIRKTASSAIKRLQLTHSHEYYVPSMSCRTVIYKGLLLAGQVGI
                     YFRDLADPRAVSALALAHQRFSTNTFPEWPLAHPYRMVAHNGEINTVKGNFSWMRARE
                     GVMKSPVLGDDLQKLYPISFEGQSDTATFDNTLELLTMAGYPLAHAVMMMIPEPWEQH
                     TLMDPRRRAFYEYHAAMLEPWDGPAAIAFTDGRTIGATLDRNGLRPSRYIVTNDDLVV
                     MASESGVLPIDESRIVKKWRLQPGKMFLIDLDQGRLIHDEELKQQFASAKPYRQWIDN
                     VRVPLESLPAETQPVVYRESLLDRQQAFGFSQEDIKFLLDPMAVTGEEGVGSMGNDSP
                     LAVLSSRNKPFFNYFKQLFAQVTNPPIDPIREA"
     gene            complement(1759..2445)
                     /locus_tag="PLW13_11755"
     CDS             complement(1759..2445)
                     /locus_tag="PLW13_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY71097.1"
                     /translation="MARMSRLAVAGHPHHLFQRGNDRTALFRDDADRERYLDCLREAA
                     VAAKVALHAYVLMDDHVHLLATPAGTEGLSRMMQALGRNYVGWFNHRHQRSGTLWEGR
                     FRCGLIEADTWLLRCMRYIELNPLRAGIAAEAGAFRWSSARHHLGQATDALLTDHALF
                     WSLGNTPFEREAAWRDFLGQPVLVAEAQQITASCLRGLALGSAGFLERMSAVSGRRLV
                     PAKRGRPRKH"
     gene            2612..3487
                     /locus_tag="PLW13_11760"
     CDS             2612..3487
                     /locus_tag="PLW13_11760"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQY71098.1"
                     /translation="MSDDGLLRLPTAEEAEAFWRDGVVCLRGVLDPAYVLAMAPAVER
                     LIRESLGTTMVDMSAMGEELARAGETVLSDARAGGGRFVSGIDHWRVDADCARFACRS
                     AVPAIVARLLNATRLNLWEDSILVKEPGTPERTAWHQDLSYFHVSGEQVCTTWIPLDL
                     ADAETGAMRFARGSHRWADLYRPNMFVSNMPMVGTVGEQVPDIDAMADAGAVELLQFA
                     LGPGDMTVHHARTLHAAGGNLSRERRRRAISLRYCGDDARYHFRPGAPRKPHHDFVHE
                     GEALGGPDCPEVWRA"
     gene            3553..3993
                     /locus_tag="PLW13_11765"
     CDS             3553..3993
                     /locus_tag="PLW13_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HQY71099.1"
                     /translation="MTALLVILHVLGATVWTGGHLVLALAVLPRVLRERAPAELLRFE
                     SGFEKIGIPALLLQVLTGVLLAARMLPPALWLELHNPVARLVCIKLGLLALTALLAVD
                     ARLRIIPRLSASNLRALAWHIVPVTLIAVLFVITGVGFRFGWLA"
     gene            3990..5075
                     /locus_tag="PLW13_11770"
     CDS             3990..5075
                     /locus_tag="PLW13_11770"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY71100.1"
                     /translation="MSAAAHALCRALGISLPIIQAPMAGVQGSALAIAVSGAGALGSL
                     PCAMLGPAAIASELQAIRGATRQPYNVNFFCHAPPEADDARQARWREALRPYYAEFGL
                     DPDHATPTPPRLPFDAETLDAIAPFAPPVVSFHFGLPAPPLLQRVKAWGATVLASATT
                     LEEALWLERHGADVIIAQGLEAGGHRGMFLEGDITTQLGTFALVRGIVRAVRTPVIAA
                     GGIADRAGIGAALALGAAGVQLGTVYLLCPESTTSAVHRAALQSPAARHTALTNVFTG
                     RPARAIVNRVMRERGPMSTLAPAFPLAIGAIAPLRAAAEARGSGDFSPLWAGQDTSGC
                     RAVAAAELTRELGADWDATGAKTTRNA"
     gene            5129..6991
                     /locus_tag="PLW13_11775"
     CDS             5129..6991
                     /locus_tag="PLW13_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQY71101.1"
                     /translation="MSGDNKPVASLTLGAMGVVYGDIGTSPLYAFKECFHDAHGLEPG
                     RAEIFGVLSLIFWSVMLVVTLKYVIFMMRADNRGEGGTLSLLTLATKATRSPRLSALL
                     GVLGIFAAALFYGDSMLTPAISVLSAVEGLGLVAPALGHFVVPVTLAIICALFMIQRH
                     GTASVGTFFGPVMSVWFAVLAGLGVWHIAQAPEILAALNPMHVVHLFQQHRLVAYIAL
                     GSVVLSLTGAEALYADMGHFGKRPIRIAWTAFVSPALVLNYFGQGALVLRDPALVENP
                     LYRMVPGAALLPLIVLAALATVIASQAVISGAFSVTRQAFQLKLLPRIATIHTSASEE
                     GQIYIPFINWSLFIAVVALVLGFRSSSALAAAYGVAVTGTMLIDSLLLAVVMLLTWSW
                     NRALTAACAVTFIVVDLAFFTSNALKIPHGGWFPLVIAVVIFTLLTTWRTGRRLLIAQ
                     LREAALPVELFLHGLRDVPRVPGTAVFLTSNAEGAPPALLHNLKHNKVVHETVILLTV
                     HTVDYPGTQAAERLVLNELGQGFHRAEMYFGYRDDQDVPQTLAQLAPATLPLDPMQTS
                     YFLSRETLLPARKPGMLLWREHLFAWMVRNAATPLRTFHLPPNRVVELGQQITI"
     gene            7039..7785
                     /locus_tag="PLW13_11780"
     CDS             7039..7785
                     /locus_tag="PLW13_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQY71102.1"
                     /translation="MQLHLQTWPAIEHYLRDNRTIIMPIGSTEQHGPNGLLGTDAITA
                     EAIARGVGESTGTLVAPVIAVGMAQHHLAFPGSMALRPGTLIAVIRDCVESLALHGFE
                     RFFFINGHGGNVATATAAFSEIYAAASFGGGAGTRLRCTLRNWYETPGVATLSRELYG
                     EQEGSHATPSEVALTWHLHPDRAMPMNLDPPLAPRGSFTDAADLRRNFPDGRIGSNPG
                     LATIGHGKRIYDAAVNDLSRELHRWTCPSS"
     gene            complement(7827..7952)
                     /locus_tag="PLW13_11785"
     CDS             complement(7827..7952)
                     /locus_tag="PLW13_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71103.1"
                     /translation="MQTLFIMLVGMLVAGVGIALGIVARDEALARQAHARCSPDE"
BASE COUNT         1239 a         2916 c         2735 g         1369 t
ORIGIN      
        1 gcctcgcgga tcgggtcgat cggcgggttg gtgacctgcg cgaacagctg cttgaaatag
       61 ttgaagaacg gcttgttgcg cgaggacagc accgccaacg gcgagtcgtt gcccatcgag
      121 cccacgcctt cctcgccggt gaccgccatc gggtcgagca ggaacttgat gtcctcctgg
      181 ctgaagccga aggcctgctg gcggtcgagc agcgactcgc ggtacaccac cggctgcgtt
      241 tcagcgggca gcgactcgag gggcacgcgc acgttgtcga tccactgccg gtagggcttg
      301 gccgaggcga actgctgctt gagttcctcg tcgtggatca ggcggccctg gtcgagatcg
      361 atcaggaaca tcttgcccgg ttgcaggcgc cacttcttca cgatgcggct ctcgtcgatc
      421 ggcagcacgc ccgactcgga ggccatcacc acaaggtcgt cattggtcac gatgtagcgc
      481 gaggggcgca ggccgttgcg gtccagcgtg gcgccgatcg tgcgcccgtc ggtgaaggcg
      541 atcgcggcgg ggccgtccca cggctcgagc atcgcggcgt ggtactcgta gaacgcgcgc
      601 cggcgcgggt ccatcagcgt gtgctgctcc cacggctcgg ggatcatcat catcaccgcg
      661 tgcgccagcg ggtagccggc catcgtcagc agttccagcg tgttgtcgaa ggtggcggtg
      721 tccgactggc cctcgaagct gatcgggtag agcttctgca ggtcgtcgcc gagcaccggc
      781 gatttcatca cgccttcgcg cgcgcgcatc cagctgaagt tgcccttgac cgtgttgatc
      841 tcgccgttgt gcgcgaccat gcggtagggg tgcgccagtg gccattccgg gaaggtgttg
      901 gtggaaaagc gctggtgcgc cagcgccagc gccgacaccg cgcgcggatc ggcgaggtcc
      961 ctgaagtaga tgcccacctg gccggccagc agcagtccct tgtagatcac cgtgcggcaa
     1021 ctcatgctcg gcacgtagta ctcgtggctg tgcgtgagct gcagccgctt gatcgcgctc
     1081 gaggcggtct tgcggatcac gtagagctta cgctccagtg cgtccggcac gatgatgtcc
     1141 tgcccccggc cgatgaagat ctggcggatc accggctcct tggcgcgcac cgtgggcgac
     1201 atcggcatct cgcgatcgac cggcacgtcg cgccagccca gcagcacctg cccttcggcg
     1261 cgcaccgcgc gctcgagctc ctgctcgcag gcgaggcgcg aggcgtgctc cttgggcagg
     1321 aacaccatgc ccacgccgta ctcgccgggc ggcggcagct gcaacccctg cttcgccatc
     1381 tcctcgcgat agagctcgtc gggaatctgg atcagcacgc ccgcgccgtc gcccatcagc
     1441 ttgtcggcac cgaccgcgcc gcgatggtcg aggttctcga ggatcttcag cccctgctcg
     1501 atgatcgagt gggaacgctg gcccttgatg tgcgccacga agcccacgcc gcaggcgtcg
     1561 tgctcgcggc tcgggtcata gagaccgtga cggatggcgt gctggatttc agaagccgac
     1621 atgactgacc tcgcgtgcgg gaggattcgt tggaggcggc agcatactcc acgcgcccgg
     1681 cagggccaag catattaaat agggtcggag ttgatttaaa acaacatgct ctgtctgcga
     1741 gatttatatg gggacacatc aatgcttgcg cggtcgtccc cgctttgccg gcacgagccg
     1801 tcgccccgac accgcgctca tccgctccag gaagccggcg gaacccagcg ccaggccccg
     1861 caggcaggac gccgtgatct gctgtgcctc cgccaccagc accggttgcc cgaggaagtc
     1921 ccgccacgcc gcctcgcgct cgaagggcgt gttgcccaac gaccagaaca acgcgtgatc
     1981 ggtcagcagc gcatcggtgg cctgcccgag atgatgacgc gcgctggacc agcggaatgc
     2041 ccccgcttcg gcggcgattc cggcgcgcag cgggttcagc tcgatgtagc gcatgcagcg
     2101 cagcagccag gtatcggcct cgatcaggcc gcagcgaaag cgcccctccc acaacgtgcc
     2161 gctgcgctgg tggcgatggt tgaaccagcc cacgtagttg cgacccagcg cctgcatcat
     2221 gcggctcaaa ccctcggtcc cggcgggcgt ggccagcagg tgcacgtgat cgtccatcaa
     2281 cacgtaggca tgcagcgcga ccttcgccgc caccgccgcc tcgcgcaggc agtcgaggta
     2341 gcgctcgcgg tcggcgtcgt cgcgaaacag cgcggtgcgg tcgttgccgc gctggaacag
     2401 gtgatgcggg tggccggcga ctgccagccg ggacatgcgc gccatggggg gtacggatcc
     2461 ggaaccggga cggggcgtcc agtataggcg cagcgcgcag cgcatcatcg agattgtaat
     2521 cggcgccccg gcggtgtgaa atcagtggct tgtcgatcca acccactcgc cgcccggcaa
     2581 tggcggcggc acgaacccgg agaccacccg catgagcgat gacggactgc tgcgcctgcc
     2641 caccgccgag gaagccgagg ccttctggcg cgacggcgtg gtctgcctgc gcggtgtgct
     2701 ggaccccgct tacgtgctgg cgatggcgcc ggcggtggaa cggctgatcc gcgaatcact
     2761 gggcacgacc atggtcgaca tgagcgccat gggcgaggag ctcgcgcgcg ccggcgagac
     2821 cgtgctgagc gacgcgcgtg ccggcggcgg acgcttcgtc tcgggcatcg accactggcg
     2881 cgtggacgct gattgcgccc gcttcgcctg ccgctccgcg gtaccggcca tcgtcgcacg
     2941 gctgctcaac gccacgcgcc tcaacctctg ggaggacagc atcctggtga aggagcccgg
     3001 cacccccgag cgcaccgcct ggcaccagga cctgtcgtac ttccacgtca gcggcgagca
     3061 ggtctgcacc acctggatac cgctggatct ggccgacgcc gagaccggcg cgatgcgctt
     3121 cgcgcgcggc tcgcaccgct gggccgacct gtaccgcccg aacatgttcg tctccaacat
     3181 gccgatggtc ggcacggtgg gcgagcaggt gccggacatc gacgccatgg cagacgcggg
     3241 cgccgtggag ctgctgcaat tcgcgctggg ccccggcgac atgacagtac atcacgcgcg
     3301 cacgctgcac gcggccggcg gcaacctgag ccgcgagcgc cgtcgccgcg cgatcagcct
     3361 gcgctactgc ggcgatgacg cgcgctacca cttccgtccc ggtgcgccac gcaagccgca
     3421 tcacgacttc gtgcacgagg gcgaggcgct cggcggcccc gattgccccg aggtctggcg
     3481 ggcctgaatc ccgatggtgc cgcgccggcg actgcgctat ggtcggcgga tcgtcctgca
     3541 cagggcttcg ccatgaccgc tctgctcgtg atcctccatg tgctcggcgc caccgtctgg
     3601 accggcggcc acctcgtgct cgcgctggcg gtgctgccgc gcgtgctgcg cgagcgcgca
     3661 cctgccgagc tgctgcgctt cgagtcgggc ttcgagaaga tcggcatccc ggcgctgttg
     3721 ctgcaggtgc tcaccggcgt gctgctcgcc gcgcggatgc tgccgccggc gctctggctc
     3781 gagctgcaca acccggtggc acgcctggtc tgcatcaagc tcgggctgct cgcgctgacg
     3841 gcactgctgg ccgtggacgc gcggctgcgc atcatcccgc ggctcagcgc gtcgaacctg
     3901 cgcgcgctcg cgtggcatat cgtgccggtc acgctgatcg cggtgctgtt cgtgatcacg
     3961 ggcgtgggct ttcgtttcgg ctggctggcg tgagcgcagc cgcgcacgcg ctgtgccggg
     4021 cgctcggcat ctcgctgccg atcatccagg cgccgatggc cggagtgcag ggcagcgcgc
     4081 tggcgatcgc cgtgagcggg gccggcgcgc tgggctccct gccctgcgcg atgctgggtc
     4141 ccgcggcgat cgcctccgaa ctgcaggcaa tacgtggcgc cacccggcaa ccgtacaacg
     4201 tcaatttctt ctgccacgcg ccaccggagg cggatgacgc gcgccaggcg cgctggcgcg
     4261 aggcgttgcg cccgtactac gccgaattcg gcctcgatcc ggaccacgca accccgacgc
     4321 cgccacgcct gcccttcgat gccgagacgc tggacgcgat cgcgcccttc gccccgcccg
     4381 tagtcagctt tcatttcggc ctgcccgcgc ctcccctgct gcagcgcgtg aaagcctggg
     4441 gcgcgacggt gctggcatcg gccaccacgc tcgaggaggc gctgtggctg gaacgccatg
     4501 gcgccgacgt gatcatcgcg cagggactgg aggccggcgg ccatcgcggc atgttcctcg
     4561 aaggcgacat caccacgcag ctcggcacct tcgcgctggt gcgcggcatc gtgcgcgccg
     4621 tgcggactcc cgtgatcgcc gccggcggca tcgccgatcg cgccggcatc ggcgcggcgc
     4681 tggcgctggg cgccgccggc gtgcagctcg gcaccgtcta cctgctctgc cccgagtcca
     4741 cgaccagcgc cgtgcaccgg gccgcgctgc agagtccggc ggcacgccac accgccctca
     4801 ccaatgtgtt caccgggcgc ccggcgcgcg ccatcgtgaa tcgcgtgatg cgcgagcgcg
     4861 ggccgatgag cactctcgcg ccggcatttc cgctcgccat cggcgcgatc gcgccgctgc
     4921 gcgccgccgc cgaggcacgc ggcagcggtg acttctcgcc gctatgggcg ggccaggaca
     4981 ccagcggctg ccgcgccgtc gccgcagcag agctcacgcg cgagctgggt gcagattggg
     5041 acgccacggg tgcaaagacc acacgaaacg cgtaggcttt gcatcgctca tgtgaccgca
     5101 tggcgcgctc tcgaggaatc ctgttttcat gtccggcgac aacaagcccg tagcgtccct
     5161 gacccttggt gccatgggcg tggtttacgg cgacatcggc accagtccgc tgtacgcctt
     5221 caaggaatgc ttccacgacg cgcacgggct ggagcccgga cgcgccgaga tcttcggcgt
     5281 gctgtcgctg atcttctggt cggtcatgct ggtcgtgacg ctgaaatacg tgatcttcat
     5341 gatgcgcgcc gacaatcgcg gcgaaggcgg cacgctgtcg ctgctgacgc tggcgacgaa
     5401 agccacgcgc agtccgcgcc tctccgcgct gctgggggtg ctcggaatat tcgccgccgc
     5461 gctcttctac ggcgacagca tgctcactcc cgcgatctcg gtactcagcg ccgtcgaagg
     5521 cttgggactg gtcgcacccg cgctcggcca tttcgtagtc ccggtgacgc tggccatcat
     5581 ctgcgccctg ttcatgatcc agcgccacgg caccgcttcc gtcggcacct ttttcgggcc
     5641 ggtgatgtcg gtgtggtttg ccgtcctcgc cggactcggc gtctggcaca tcgcgcaggc
     5701 acccgaaatc ctggccgcgc tgaatcccat gcacgtggtg cacctgttcc agcagcaccg
     5761 gctggtcgcc tacatcgcgc tcggctcggt ggtgctgtcg ctcaccgggg ccgaggccct
     5821 ttacgccgac atgggccact tcggcaagcg cccgatccgc atcgcgtgga ccgcgttcgt
     5881 ttcgccggca ctggtgctca actacttcgg gcagggcgcg ctggtgttgc gcgacccggc
     5941 gctggtggag aatccgttat accgcatggt gcctggcgcc gcgctgctgc cgctgatcgt
     6001 gctcgccgcg cttgcgaccg tgattgcctc gcaggcggtg atatcgggcg cgttctcggt
     6061 aacacggcag gccttccagc tgaagttgct gccgcgcatc gccacgatcc acacctccgc
     6121 atccgaagaa ggccagatct acatcccgtt catcaactgg agcctgttca tcgccgtggt
     6181 cgcgctggtg ctcggattcc gcagttccag cgccctcgcc gcggcctatg gcgtggcggt
     6241 cacaggcacc atgctgatcg actcactgct gctggccgtg gtgatgctgc tgacgtggtc
     6301 gtggaaccgc gcgttgaccg ccgcttgcgc cgtcacgttc atcgtggtcg atctcgcctt
     6361 cttcacctcg aacgcgttga agatcccgca tggcggctgg ttcccgctgg tgatcgccgt
     6421 ggtgatcttc acgctgctga cgacctggcg cacgggtcgc cggctgctga tcgcgcagtt
     6481 gcgcgaagcg gcgcttcccg tcgagctgtt cctgcacggc ttgcgcgatg tgccgcgcgt
     6541 ccccggcacc gccgtgttcc tcaccagcaa tgccgagggt gcacccccgg cgttgctgca
     6601 caacctcaag cacaacaagg tggtgcacga gaccgtgatc ctgctgaccg tgcacacggt
     6661 cgactacccc ggcacgcaag ccgcggagcg cctggtgctc aatgaactcg gccagggttt
     6721 ccatcgcgcc gagatgtact tcggctatcg cgacgaccag gacgtgccgc aaacactggc
     6781 gcagctcgca cctgccacgc tgccgctcga cccgatgcag acctcctact tcctgagccg
     6841 cgaaacgctg ttgcccgcgc gcaagcccgg catgctgctg tggcgcgagc acctgttcgc
     6901 ttggatggtg cgcaatgccg caaccccgct gcgcacgttc catctgcccc cgaaccgggt
     6961 cgtggaactt ggacagcaga tcacgatctg aggaccgcaa cgccggcgcc gactcacccg
     7021 atcaggaacg attgcccgat gcaactgcac cttcagacct ggcccgcgat cgagcactac
     7081 ctgcgcgaca atcgcaccat aatcatgccg atcggctcca ccgagcagca cggcccgaac
     7141 ggcctgctcg gcaccgatgc gatcaccgcc gaggccatcg cgcgcggcgt cggcgagtcg
     7201 accggcacac tcgtggcgcc ggtcatcgcg gtcggcatgg cgcagcatca cctcgcgttc
     7261 cccggctcga tggcgctgcg cccgggcacg ctgatcgcgg tgatccgcga ctgcgtcgag
     7321 agcctggcgc tgcacggctt cgagcgcttc ttcttcatca atggtcatgg cggcaacgtc
     7381 gccacggcca cggccgcctt ctcggagatc tacgccgcgg ccagctttgg cggcggcgca
     7441 ggcacccggc tgcgctgcac gctgaggaac tggtacgaaa cgcccggcgt cgccacgctg
     7501 tcgagggagc tttacgggga acaggaaggc agccacgcca cgccgtcgga agtggcgctg
     7561 acctggcacc tgcatccgga ccgcgccatg cccatgaacc tcgatccgcc gctagctccc
     7621 cgaggctcgt tcaccgacgc cgccgatctg cgccgcaact tccccgacgg ccgcatcggc
     7681 tccaaccccg gtctcgcgac gatcgggcac ggcaagcgca tctacgacgc cgccgtgaac
     7741 gacctcagcc gggaattgca ccgctggact tgcccctctt cctgaagagc gcccacaggg
     7801 attaaagcca gtcgctcaca atcggatcac tcgtcgggcg agcagcgggc gtgggcctgg
     7861 cgtgcgagcg cttcgtcgcg cgcgacgatg ccgagcgcga tgccgacacc ggccaccagc
     7921 atgccgacca gcatgatgaa cagcgtttgc atgaactacc tccacgaatt gcacgccttg
     7981 ccggggctac atccgcgaca cctgccacgg cgccacgaaa gccattccat ggccacccgc
     8041 accacgccac cccccggcag cgcgctccgt gccccgcatc cttgcgcagc cggatacgcc
     8101 aacggtggcg ggatggatca gcgcggcgcg cggtgcgcgc cgtggttcag aggttgtcgc
     8161 ataggccaag gcgttgcgag cgagcggtct ggtgcgtcgc tggctgtgcc gcatcgggca
     8221 gatccgggaa tgcgcgtttc caccacggcg aggggctcg
//
LOCUS       DAORUQ010000141         8229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9667_length_8229_cov_5.414730, whole genome shotgun sequence.
ACCESSION   DAORUQ010000141 DAORUQ010000000
VERSION     DAORUQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9667_length_8229_cov_5.414730"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1166)
                     /locus_tag="PLW13_11790"
     CDS             complement(<1..>1166)
                     /locus_tag="PLW13_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY71104.1"
                     /translation="YPGASPEAIENDLLKPIENAINTVDGIQRIYATAREGSGYMMVE
                     FRFDTDMAAATQELRDKIAQLAPALPEDAEDPLVSRTNEQDNAAPVVNLSIHSKTRSL
                     RELSTLTEQVVVKRLQNVAGVGSVVVNGAVARQIKVLLRPEQMRAHGVGVDEVIAAIQ
                     AANQDLPAGSIRRGNSEQLVRVEGRIRDPREFGRIIVTTRGGAMYLQQGGLPIHLDQV
                     AEIVDGEAEPESIARLNGAPALSLNIFKVQGSNVVAVGDGVRDAVEELTKRLPPDVEF
                     TIVQSSAETVKAQLSSVKMTILEGAALTVFIVFLFLHSWRSTIITGLTLPISVIATFI
                     VLYACGFTLNFVTLMALSLCIGLLIDDAIVVRENIVRHLGMGKSHRAAAQEGTE"
     assembly_gap    1167..1266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1267..1955)
                     /locus_tag="PLW13_11795"
     CDS             complement(<1267..1955)
                     /locus_tag="PLW13_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY71105.1"
                     /translation="MATATGPGKGRVTALLTLLAGAWLFAGCSGDADVPAAQAADTTA
                     STLELAADDVVTAERRVLARRLPISGSLTPLVQSQIRSRVAGEVLEVSRREGEAVRRG
                     EVLVRIDTRSQEAQVASQKAALDKARADLGIARLNVENSERLLAKKFIAQNVLDTQTS
                     VYAAALASVKLAEAQLRLAEIGLADAVVRAPFDAVVARRFAEPGEKVSSDSALLELVD
                     LSRLELQAAAPA"
     gene            complement(2207..2824)
                     /locus_tag="PLW13_11800"
     CDS             complement(2207..2824)
                     /locus_tag="PLW13_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71106.1"
                     /translation="MPTDASLRPFVGRVLLLLPFAFALWYVTAAWHLAPVVWLSELIL
                     RSWMPEAVAGLRLQGGALLLVTHFGEVAGRLVADPPPGENLGFLSNPLSYSYSLPVFA
                     ALALATPGPREWWRLLAGLAILTLVAVASMLATLLKTLAFDVGPVFVAQQQWGALARN
                     GAAMAYQFGSLLLPMVTPVVLWLALYRGYLAELAPGIASRWRGAR"
     gene            complement(2860..3363)
                     /gene="xrtH"
                     /locus_tag="PLW13_11805"
     CDS             complement(2860..3363)
                     /gene="xrtH"
                     /locus_tag="PLW13_11805"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HQY71107.1"
                     /translation="MGRFLFLFVFLLAAMFAIEMLQPMQRHVVLPWTSLLADASGWLM
                     RLFDDQVAVSGKIISSTVKPFSVSIEPGCNGVEAMIVLLAAILAVRAPLGYKLMGLLG
                     GFVAIQGLNLVRIISLFYLGLWDRESFEWAHLYVWQALIILDALVVFLVWARFLPPEP
                     RSGVVDA"
     gene            complement(3579..4484)
                     /locus_tag="PLW13_11810"
     CDS             complement(3579..4484)
                     /locus_tag="PLW13_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71108.1"
                     /translation="MSELSGKVAIVTGAGRGLGRVEAIQLARQGARVIINEIGLPAAR
                     EAAESVKEEIKAFGGEALTVYGDCADWNDSEALFKTAIDTFGDVNIVVNNAGFCRDKM
                     IFTMSEEEFDSVVRVHLKGHFVNMRHAAAYWREKAKSSGGTVYGRLVSTSSEAFLYGS
                     VGQPNYAAAKAGIVAMTMGAAQALVKYGVTANVIMPRARTDMTMSGGTAAMFEKPAEG
                     FDHFDPAHVAPFVGYLASPRSQRISGYVFVVWGKQVTVVDGPKLGTVFENDKQWTVDA
                     LDTAMSPHFEKLRPILDGFAVDPMA"
     gene            complement(4506..5681)
                     /locus_tag="PLW13_11815"
     CDS             complement(4506..5681)
                     /locus_tag="PLW13_11815"
                     /inference="COORDINATES: protein motif:HMM:NF005889.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HQY71109.1"
                     /translation="MREAVIVEALRTPIGRGKPVIGDLFGFHPAHLLAIAQRAVIEKA
                     GVKPEEVEQLIGGCVTQAGEQAGNLCRNAWLSMGTSYVPGATTIDTQCGSAQQANHLI
                     SALVASGSIDIGIACGAEVMSHVGLGANVYNGPGFFQPPNWPWDGSPDQFGSAERIAK
                     NRGITRADVDAFALESQRRAAAAQAAGYFDREIIPVDAPVFGEDGKPTGATKRVTRDQ
                     GVRATTPEGLAGLKPVAEGGIHTAGNSSQVSDGACAVLWMTREEAERRGLKPRARIIC
                     GVTVGADPYYHLDGPIDATNALFRKTGMNMGDIDLVEINEAFAAVVLSWASVFKPDMA
                     KVNRNGGAIALGHPVGSTGARLITTALHELERTDKTTALITMCCGASIGTGTIIERI"
     gene            5934..6398
                     /locus_tag="PLW13_11820"
     CDS             5934..6398
                     /locus_tag="PLW13_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQY71110.1"
                     /translation="MKQYRFDDVEALQELVSTEFGPWSNELEMTQELIDRFAELSGDD
                     YWIHTDPERCKTQSPFGTTIAHGFLTLILLPRLKTPQGYEITGFNNMLNYGSDKLRFT
                     GAVPTGSRIHARNRVKSIERLPKGTRFTLEQNVHVVGQERPALVYELVFIYQ"
     gene            complement(6403..7212)
                     /locus_tag="PLW13_11825"
     CDS             complement(6403..7212)
                     /locus_tag="PLW13_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY71111.1"
                     /translation="MSGARYLVIVDPAATGHPAFDRALLAARDSRGHIHLFGFSSHPP
                     LAANGESSSDAQERYRSEALATLGELAAHAEAQGIGVSMELGSSADVRQEMVQAAARS
                     RADMVFKYSSERTLAHRDPGDWLLMRLAPCPVLMVKNVRDWSSRRVLGAVNFGSHDLA
                     HIKLNNQIIGEAQRFAHDFGSEAHFVNAYADQNRAPDRIELARVCGVAPERMHTVQGA
                     AASVIRDTAERIGADLILIGTVARSGLMGTVVGNTAERILDETRSDVLVLN"
     gene            complement(7310..8164)
                     /locus_tag="PLW13_11830"
     CDS             complement(7310..8164)
                     /locus_tag="PLW13_11830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71112.1"
                     /translation="MGSKVAFITGASRGIGKASAIALAQKGFDVVVTARTLEEGEVYE
                     HGSRDSEVKPMSGSLRATAAEIENLGRRALPIRLDLLDLASIDAAVERVYAEWGRIDL
                     LVNNGIYQGPGIMSPVDELTQQHLHDIFRGNVFANVHTIQRVLPRMLAQGEGCIINVV
                     SESGMIDPPAAAGKGGWGFAYGASKAALIRMAGVLKVEYADTPLNFFNMEPGLVMTEA
                     MDMRPDREDFINFYGGAPMKVPAAVVAWLATAPQARELNGQTIMAQRHCLKLNLVPEW
                     QPRRRSKS"
BASE COUNT         1336 a         2808 c         2684 g         1301 t
ORIGIN      
        1 tcctcggtgc cttcctgcgc cgcggcgcgg tggctcttgc ccatgccgag gtggcgcacg
       61 atgttctcgc gcaccacgat ggcgtcgtcg atcagcagcc cgatgcagag gctgagcgcc
      121 atcagcgtga cgaagttcag ggtgaagccg caggcgtaca gcacgatgaa ggtcgcgatc
      181 accgagatcg gcagcgtgag accggtgatg atcgtggagc gccaggagtg caggaacagg
      241 aacacgatga acaccgtgag cgccgcgccc tcgaggatgg tcatcttgac gctggagagc
      301 tgcgccttga cggtctcggc gctcgactgc acgatcgtga attcgacgtc cggcggcagg
      361 cgcttcgtca gttcctccac ggcatcgcgc acgccgtccc cgaccgcaac cacgttcgat
      421 ccctgcacct tgaagatgtt gagactgagc gcgggcgcac cgttcaggcg cgcgatcgac
      481 tcgggctccg cctcgccatc gacgatctcg gccacctggt cgaggtggat cggcagcccg
      541 ccttgttgca ggtacatcgc gccgccgcgc gtggtgacga tgatgcggcc gaactcgcgc
      601 gggtcccgga tgcggccctc cacgcgcacc agctgctcgc tgttgccgcg gcggatggaa
      661 cccgccggca ggtcctggtt cgcggcctgg atcgccgcga tcacctcgtc cacgcccacg
      721 ccgtgcgcgc gcatctgctc gggtcgcagc agcaccttga tctggcgcgc cacggcaccg
      781 ttgaccacca cgctgcccac accggccacg ttctgcaggc gcttcaccac cacctgctcg
      841 gtcagcgtcg agagttcgcg cagcgaacgc gtcttggagt ggatcgagag attcaccacc
      901 ggagccgcgt tgtcctgctc gttggtgcgc gagaccagcg ggtcctcggc gtcttccggc
      961 aacgcggggg cgagctgcgc gatcttgtcg cgcagttcct gcgtcgccgc ggccatgtcg
     1021 gtgtcgaacc tgaactcgac catcatgtag ccggagccct cgcgcgccgt ggcatagatg
     1081 cgctggatgc cgtcgaccgt gttgatggcg ttctcgatcg gcttcagcag gtcgttctcg
     1141 atcgcctcgg gcgaggcgcc cgggtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnngcgg gcgccgcggc ctgcagctcg agccgcgaca ggtccacgag ttcgagcagc
     1321 gcggaatcgc tggacacctt ctcgccgggt tcggcgaagc ggcgcgccac caccgcgtcg
     1381 aagggggcac gcaccacggc atcggcaagc ccgatctcgg cgagacgcag ctgcgcctcg
     1441 gccagcttca cgctggcaag agccgcggcg tagacacttg tctgggtgtc gagcacgttc
     1501 tgcgcgatga acttcttcgc cagcaggcgc tcgctgttct cgacattgag ccgcgcgatg
     1561 cccagatccg cgcgcgcctt gtccagcgcc gccttctggc tcgcaacctg cgcctcctgg
     1621 ctgcgcgtgt cgatgcgtac cagcacctcg ccgcggcgca ccgcctcgcc ctcgcggcga
     1681 ctgacctcga gcacctcgcc ggccaccctg gacctgatct ggctctgcac cagcggcgtc
     1741 agcgaacccg agatgggcag tcggcgcgcc agcacgcgcc gctcggcggt cacgacgtca
     1801 tcggcggcca gttcgagcgt gctggccgtc gtgtccgcgg cctgcgccgc aggcacgtcg
     1861 gcatcgcccg agcacccggc gaacagccag gcgcccgcga gcagcgtcag cagcgctgtg
     1921 acacgcccct tcccgggacc tgttgctgtt gccatgtgga agcttccccg caattctcgg
     1981 cccgattccg cattacgcgg accacaccac ggcggatctg gagccatccg ccggaccacc
     2041 atgatgcacc gcaagactac gggctttgcg tggcggagtt cggcaaaatg cacgtcccgc
     2101 gcaggtccgg cttcatccgg acgatgtcag ctcgaagagg gtgtcgcaaa cgccatccgg
     2161 ttgcgagcga gcggcctggt gcgtcgctgg ctgtgctgcg cgccggtcag cgcgccccgc
     2221 gccaacgcga ggcgatcccg ggtgcgagct cggcgagata accccggtac agagcaagcc
     2281 acagcaccac gggcgtcacc atgggcagca gcagcgagcc gaactgatac gccatcgcgg
     2341 caccgttgcg ggccagcgcg ccccattgct gctgcgccac gaagacgggt cccacgtcga
     2401 aggccagggt cttgagcagc gtcgcgagca tcgaggccac tgccaccagc gtcaatatgg
     2461 ccaggccggc gagcagccgc caccattcgc gcggccctgg cgtcgccagt gccagcgccg
     2521 cgaatacggg caacgagtag ctgtagctca gcgggttgct gaggaagccg aggttttcgc
     2581 ctggcggcgg gtcggccacg agccggcccg ccacttcgcc gaaatgcgtg accagcagga
     2641 gtgcgccgcc ctgcagccgc agaccggcca ccgcctcggg catccaggac cgcaggatca
     2701 gctccgacag ccagaccacc ggcgcgagat gccacgcagc cgtgacgtac cagagtgcga
     2761 acgcaaacgg gagcaggagc agcacgcgcc ccacgaacgg gcgcagcgag gcatcagtgg
     2821 gcatcgcgaa cggtcccgaa acggtttgcg cggcgattct caggcatcga caacccctga
     2881 tcgtggctcg gggggcagga agcgcgccca taccaggaac accaccagcg catccaggat
     2941 gatcaacgcc tgccatacgt acaggtgggc ccattcgaag ctctcgcggt cccagaggcc
     3001 gaggtagaac aggctgatga tccgcaccag gttgagcccc tggatggcca cgaagccgcc
     3061 cagcaatccc atgagcttgt aaccgagcgg cgcgcgtacg gccaggatgg ccgccagcag
     3121 cacgatcatg gcctcgaccc cgttgcaccc cggctcgatg gacacgctga aaggcttcac
     3181 ggtgctgctg atgatcttgc cggacacggc aacctggtcg tcgaacaggc gcatgagcca
     3241 gccgctggca tccgccagca ggctggtcca gggcaacacc acgtggcgct gcatcggctg
     3301 caacatctcg atggcgaaca tcgccgccag caaaaagacg aacaggaaca ggaatctgcc
     3361 caacaccgct ctccttggcg tttgctgtgc aggatacctg atcgccagcg gcacatgcac
     3421 acccgaccca cgctgcgaaa cgcgtcgtga cggatcggga gcgatccgcc tgcatccaat
     3481 gatgggcggg aagcgcttac ggggtttgca cgcggctggg tgagctgcac gccggcggcg
     3541 cccaggcgcc accggcatgc gatgaacggc gggaggaatc aggccatcgg gtcgacggcg
     3601 aagccgtcga ggatcggacg cagcttctcg aagtgcggcg acatcgcggt gtcgagcgca
     3661 tcgacggtcc actgcttgtc gttctcgaac acggtgccga gcttcggccc gtcgaccacg
     3721 gtgacctgct tgccccacac cacgaacacg taacccgaga tccgctgcga gcgcggcgag
     3781 gccaggtaac ccacgaaggg cgcgacgtgg gccggatcga agtggtcgaa accctcggcg
     3841 ggcttctcga acatcgcggc cgtgccgccg ctcatggtca tgtcggtacg cgcgcgcggc
     3901 atgatcacgt tcgcggtgac gccgtacttc accagcgcct gcgccgcgcc catggtcatg
     3961 gccacgatgc cggccttggc ggcggcatag ttgggctggc ccacggagcc gtagaggaaa
     4021 gcctccgagg aggtgctgac caggcggccg tacacggtgc cgccgctgga cttggccttc
     4081 tcgcgccagt aggccgcggc gtggcgcatg ttcacgaagt ggcccttcag gtgcacgcgc
     4141 accacggagt cgaattcctc ctcgctcatc gtgaagatca tcttgtcgcg gcagaaaccg
     4201 gcgttgttga ccacgatgtt gacgtcgccg aaggtatcta tcgccgtctt gaacagcgcc
     4261 tcggaatcgt tccagtcggc gcagtcgccg tagaccgtga gcgcctcgcc gccgaaggcc
     4321 ttgatctcct ccttcacgct ctcggccgcc tcgcgcgccg ccggcaagcc gatctcgttg
     4381 atgatcacgc gcgcgccctg gcgcgcgagc tggatggcct cgacgcgacc gagcccgcgg
     4441 cccgcgccgg tgacgatggc taccttgccg ctcagttcac tcattgctca ctcctcgcga
     4501 tgggttcaga tacgttcgat gatcgtgccg gtgccgatcg aggcgccgca gcacatcgtg
     4561 atcagcgcgg tggtcttgtc ggtgcgctcg agctcgtgca gcgccgtggt gatcaggcgc
     4621 gcgccggtcg agccgaccgg gtggccgagc gcgatggcgc cgccgttgcg gttcaccttg
     4681 gccatgtcgg gcttgaacac gctggcccag gacagcacga ccgccgcgaa ggcctcgttg
     4741 atctcgacga gatcgatgtc gcccatgttc atgccggtct tgcggaacag cgcgttggtc
     4801 gcatcgatcg ggccgtcgag gtggtagtag ggatccgcgc ccaccgtgac gccgcagatg
     4861 atgcgcgcgc gcggcttcag cccgcggcgc tcggcttcct cgcgggtcat ccacagcacc
     4921 gcgcaggcgc cgtcggaaac ctgcgaggag ttgccggccg tgtggatgcc gccctcggcg
     4981 accggcttca gccccgcgag cccttccggg gtcgtcgcgc gcacgccctg gtcccgggtc
     5041 acgcgtttgg tggcaccggt gggcttgccg tcctcgccga acaccggggc gtcgacggga
     5101 atgatctcgc gatcgaagta cccggcagcc tgcgccgccg cggcgcggcg ctgcgactcc
     5161 agcgcgaagg cgtcgacgtc ggcgcgcgtg atgccgcggt tcttcgcgat gcgctcggcc
     5221 gaaccgaact ggtcggggct gccgtcccac ggccagttcg gcggctggaa gaagcccggg
     5281 ccgttgtaga cgttggcgcc caggcccacg tggctcatga cctcggcgcc gcaggcgatg
     5341 ccgatgtcga tggagccgga cgcgaccagc gccgagatca ggtggttggc ctgctgcgcc
     5401 gatccgcact gggtgtcgat cgtggtggcg ccgggcacgt agctggtacc catcgacagc
     5461 caggcattgc ggcacaggtt gccggcctgc tcgccggcct gcgtgacgca accgccgatc
     5521 agctgctcca cttcctcggg cttgacgcct gccttctcga tcacggcgcg ctgcgcgatc
     5581 gccagcaggt gcgcgggatg gaagccgaac agatcgccga tgaccggctt gccgcgtccg
     5641 atgggagtac gcagggcttc aacgatcact gcttcacgca tgactgactc cttggtcgag
     5701 ggtgggccgc gacgcgcgcg gaaaacagaa agactgcgcg gcccgcccgg gtcgcgtcag
     5761 ggcgcagaga ttagccgaaa acgccgccgg aaaacgatga cattaggctt cgcgcccggc
     5821 tgacatctcc actcaggcat accgggttgc cggggtctag gtggcggcgg ggctggcggc
     5881 tatgatagcg ggctttcgcc gagaccaacc ccgccgcgcc acaggagcac ccgatgaaac
     5941 agtaccgatt cgacgacgta gaggccctcc aggaactggt gtccaccgag ttcggcccgt
     6001 ggagtaacga actggagatg acccaggaac tgatcgaccg cttcgccgaa ctctcgggcg
     6061 atgactactg gatccacacc gacccggagc gctgcaagac ccagagcccc ttcggcacca
     6121 cgatcgcgca cggtttcctc acgctgatcc tgctgccgag gctgaagacc ccgcaaggct
     6181 acgagatcac cggcttcaac aacatgctca actacggctc ggacaagctg cgcttcaccg
     6241 gcgcggtacc gacgggctcg aggatccatg cgcgcaatcg cgtgaagtcc atcgagcgcc
     6301 tgcccaaggg cacgcgcttc acgctcgagc agaatgtgca cgtcgtcggg caggagcgcc
     6361 cggcgctggt ctacgaactg gtgttcatct accagtaggc cctcagttca gcaccagcac
     6421 gtccgagcgc gtctcgtcga ggatgcgctc ggcggtgttg cccaccaccg tacccatcag
     6481 gccgctgcgc gccacggtgc cgatcaggat gaggtcggcg ccgatgcgct cggcggtgtc
     6541 gcgaatgacg ctggccgccg cgccctgaac cgtgtgcatg cgctcgggcg cgacaccgca
     6601 gacgcgcgcc agctcgatgc gatccggtgc gcggttctgg tccgcgtagg cgttcacgaa
     6661 atgcgcttcc gaaccgaagt cgtgcgcaaa gcgctgcgcc tccccgatga tctggttgtt
     6721 cagcttgatg tgcgccaggt cgtgggagcc gaaattcacc gccccgagca cgcgcctgct
     6781 gctccagtca cgcacgttct tcaccatcag caccggacac ggcgccaggc gcatcagcaa
     6841 ccagtcgccg gggtcgcggt gtgccagggt cctttcgctc gagtacttga acaccatgtc
     6901 ggcacgcgag cgcgccgcgg cctgcaccat ctcctgtcgc acgtcggccg aggagcccag
     6961 ctccatgctc acgccgatgc cctgtgcttc ggcatgggcg gcgagctcac cgagggtggc
     7021 cagcgcctcc gatcgatagc gctcctgcgc gtcgctcgaa gactcaccgt tggccgcaag
     7081 cggagggtgg ctcgagaaac cgaagaggtg gatatgcccg cgcgaatcgc gagccgcgag
     7141 cagtgcccgg tcgaaggcag gatgcccggt tgccgccggg tcgacgatca cgaggtagcg
     7201 cgcaccagac atctgggatg actccgtctc tttcgattgc ttgtttgcgg gctgaacgcc
     7261 cctgcgccgg attctttcgc gccggcggat gcccgcgcca tgaccggcat caagatttgc
     7321 ttcgtcgccg cggctgccac tccggcacca ggttcagctt caggcagtgg cgctgcgcca
     7381 tgatggtctg gccgttcagc tcgcgcgcct gcggcgccgt cgccagccac gcgacgaccg
     7441 ccgccggcac cttcatcggc gcgccgccgt agaaattgat gaaatcctcg cggtcggggc
     7501 gcatgtccat cgcctcggtc atgaccagcc cgggttccat gttgaagaaa ttgagcggcg
     7561 tgtccgcata ttccaccttg agcacgccgg ccatgcggat cagcgccgcc ttggaagcgc
     7621 cgtaggcaaa gccccagccg cccttgcccg ccgctgccgg cggatcgatc atccccgatt
     7681 ccgacaccac gttgatgatg cagccctctc cctgcgcgag catgcgcggc agcacgcgct
     7741 ggatcgtgtg cacgttggcg aacacgttgc cgcggaagat gtcgtgcagg tgctgctggg
     7801 tcagttcgtc caccggcgac atgatgcccg gcccctggta gatgccgttg ttgaccagca
     7861 ggtcgatgcg gccccactcg gcatagacgc gctcgaccgc ggcgtcgatg gaggcgagat
     7921 cgagcaggtc cagacgtatc ggcaacgcgc gccggcccag attctcgatc tcggcggcgg
     7981 tggcgcgcag gctgccggac atcggcttca cctcggaatc gcgcgagccg tgctcgtaga
     8041 cctcgccctc ctccagcgtg cgcgccgtga ccacgacgtc gaagcccttt tgcgccagcg
     8101 cgatggccga ggccttgccg atgccgcggc tcgcgccggt gatgaacgcc actttcgatc
     8161 ccatgtcggt tccgctcctg gtccgggaat tttccctaac aggctatcac gcgggcgcgg
     8221 gaaaatgcg
//
LOCUS       DAORUQ010000142         8193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9736_length_8193_cov_5.696363, whole genome shotgun sequence.
ACCESSION   DAORUQ010000142 DAORUQ010000000
VERSION     DAORUQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9736_length_8193_cov_5.696363"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PLW13_11835"
     CDS             complement(<1..1505)
                     /locus_tag="PLW13_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY71113.1"
                     /translation="MDGQWDAIVIGAGHNGLAAARVLAQRGRRVLVLEKNRYAGGMAG
                     TREIFPGCRNEVGASALFPIAPEVLDFLQFERHGVEFIELPVMAINLAGPGHKPIMFY
                     RSARRQLWHLLRHHGPAALAGFAKFAKFVAYPASVMDRFNPGCTPRSLDELLSGAPDE
                     ARREQLRLAFTGSAMDVIDRFFPDPRKHHTFRSLLAFAAIQSTYKGPYTPGSALCLVY
                     TFAQSGDSGLMRRVKGGMGSLSEALARSIEAVGGTVRLGATVDQVLLEDGRATGVVLK
                     GGETLRARVVLSNLDRPATFQRLVGERHLDETIANKVAAAEHRGAWVHMLFRLDGLPA
                     YGGEWAWLNSDVHNRFGGAMVASPEELQRSFETCDAGQLPDWLPVAFQFPSVMDPSLA
                     PAGQHVASAYGFCFPCKAPKSERGRLRDAAADKVIDTICGYLPDFRARILDRAVFSSD
                     HFASMHGATEGDFTHGLIHPENMLGERAMVPGSAHRTPIEGLWMCGSACHPG"
     gene            1592..2791
                     /locus_tag="PLW13_11840"
     CDS             1592..2791
                     /locus_tag="PLW13_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY71114.1"
                     /translation="MSTTAAIVSVLGSSGRLLADAVLDWHPRTLPRRGRAVEDAAFLN
                     RLLAEHGNDARSRAARAATVRVRATRPPSSNCSNRVLEVDWAHGEGMPRTLFLKLPGE
                     PLATRFFCNAIRVWELECAFFRSFAHDFPLRLPRVHAIATRGSRFALLQEDLGADPAV
                     RLFTNRAMLEGVPLELARRCIEAFATFHAHYHGLDAQARERRLPLARHPFLSPHMSAI
                     SQAINRAAVEPCRRKAPAVFGAAQAARFRAAMGRWQDLQAWWYREPLTLVHGDSHIGN
                     FFVDGERMGMLDFQAAHWSKGIRDLQYFLVNSMRAEELACHERALVSEYAAALRARGV
                     ALADDEAWAQYRGFAFQPLMTIVTSLGLGPLTERDALMEAVLARALALIERTAFHDWL
                     DALPRAA"
     gene            2821..4113
                     /locus_tag="PLW13_11845"
     CDS             2821..4113
                     /locus_tag="PLW13_11845"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71115.1"
                     /translation="MPRPALPLPVRLLNTGFAALGRTGPRLLPLDPERLMEAAARTAG
                     LDDFGSPYFREPLARLCDSLEREARLTALGRMIARQDILRLLGNRLRWVDISRRHPEI
                     GAGRVVAPVFILGMPRTGTTSMHELLALDPQFRVPLSWEVAHPFPPPETASYRSDPRI
                     AQVDAELARVDRLLPEFRNMHPMGAELPQECVGLFAHDFVSMIFDVQFRLPAYQEWLI
                     REDMGEVFRNHRGWLQLLQWKKPGDTWVLKSPQYLWNIEDMLREYPDARVIQMHRDPV
                     TVAMSVGSLTATLRGLASDTVDLKEVTQHYADLLDFGTRRTMAARASGLLGAGRVIDV
                     QFADFRADALAEIRRIYAHLGLRLDDAVAEAMRRFLDAGRASARHARHRYDLAASGID
                     LARERERFADYQQAYGIGSEISGGSYQNGEEYARSCST"
     gene            complement(4038..5933)
                     /locus_tag="PLW13_11850"
     CDS             complement(4038..5933)
                     /locus_tag="PLW13_11850"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY71116.1"
                     /translation="MRSLKAPLLFTLLLGGCDAPEQPAATEPGAVPAATPVEAPAQPV
                     APPHPERVLFGDLHIHTSFSPDAFAVGNRTLPDDAYRFAKGEQTLHAAGYPVQLHRPL
                     DFAAVTDHSEYMGVLPQLADPQSPLSKLPLAARFMSADPGEAQTALTEVVGTLNSGEP
                     IAELVSPQVTSSVWQQIVAAANRHNDPGAFTALVGYEWTSTIDGNNLHRNVIFRGDTV
                     PERPFSSFDSPDPEQLWAWLEEKRAAGIDSLAIPHNQNASNGLMFGTRTFDGRPFDAD
                     YVSRRMRNEPVHEIIQIKGQSEVHPVLAPNDEFANFEIFPITLKMGPEPTISQPAGSY
                     VREALGVGLELDASGIGNPYHFSFIGSSDSHNSTSPIEEDNYHGKLPLIDGTPEQRLD
                     IKPASELHRGLGRIMSAQGLAAVWATGNTREAIFDALRRGETYATSGPRIGLRVFGGW
                     GYEQALLGDLAYLDKAYAGGVPMGGDLPARPADAGAPRLLIRAAKDPDGANLDRVQVI
                     KGWLDAGGQGQEKIFEVAWSGDRVPDPATGKLPPVGSTVDVAAASYTNTIGAAALEVL
                     WEDPEFDPATRAFYYVRVLEIPTPRWSTHDAAKLKVEAPAPQVLQERAYSSPFWYEPP
                     EISEPMP"
     gene            6107..7258
                     /gene="ppk2"
                     /locus_tag="PLW13_11855"
     CDS             6107..7258
                     /gene="ppk2"
                     /locus_tag="PLW13_11855"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQY71117.1"
                     /translation="MTRDRNPSQSGTQEATTPGHRPRAVQSGDVPPSMQPAALERHQA
                     DRALERAYTVQSMVVTDALSAASAHEPAVLAEVHAMLDGVSPQDARALRRMLAQRLAV
                     TGDTASDPDTALAPNWRDGAYPYRNLLSRRSYERQKYRLQVELLKLQAWVKDTGARVV
                     ILFEGRDAAGKGGAIKRFMEHLNPRGARVVALEKPSEIERGQWYFQRYIEHLPTRGEI
                     VLFDRSWYNRSGVERVMDFCTEGEYDEFLRQCPEFERQLVRSGIHLFKFWFSVSRKEQ
                     RRRFKERKLHPLKQWKLSPVDLASLDKWDDYTLAKEAMFLHCDTSDAPWTVIKSDCKK
                     RARLNAMRYVLHRMPYTRKDPSLLGPVDPLIVGRPSLVPSVGDDQLAGS"
     gene            complement(7316..7918)
                     /locus_tag="PLW13_11860"
     CDS             complement(7316..7918)
                     /locus_tag="PLW13_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HQY71118.1"
                     /translation="MKKPEHDTDTSNDYRLEPLPSLIFSSRWLQLPLYLGLIVAQGVY
                     VVLFVKELLHLVHGAFEMNEQQVMLIVLALIDVVMISNLLMMVIVGGYETFVSRLRLE
                     KHPDQPEWLSHVNASVLKVKLAMAIIGISSIHLLKTFIEAGMVGAYPLCSTVEAGVRC
                     ATATQEGIILQTVIHTVFILSAIGIAWTDRIMNGTKPADH"
BASE COUNT         1278 a         2759 c         2852 g         1304 t
ORIGIN      
        1 cccgggtggc aggcggagcc gcacatccac aggccctcga tcggggtgcg gtgcgcggag
       61 cccggcacca tcgcgcgctc gccgagcatg ttctcgggat ggatcaggcc atgggtgaaa
      121 tcgccctcgg tcgcgccgtg catgctcgcg aagtgatccg aggaaaacac cgcccggtcg
      181 aggatgcgcg cgcggaaatc gggcaggtag ccgcagatgg tatcgatgac cttgtccgcg
      241 gccgcatcgc gcaggcgccc gcgctcgctc ttcggcgcct tgcacgggaa acagaagccg
      301 taggcgctgg cgacatgctg gcccgccggc gccaggctcg ggtccatcac cgaagggaac
      361 tggaaggcga ccggcagcca gtcgggcaac tgcccggcat cgcaggtctc gaagctgcgc
      421 tgcagttcct cggggctcgc gaccatcgcg ccgccaaagc ggttgtggac gtcgctgttg
      481 agccacgccc actcgccgcc gtaggcaggc agcccgtcga ggcgaaacag catgtgcacc
      541 caggcgccgc ggtgctcggc cgctgccacc ttgttcgcga tggtctcatc gaggtggcgc
      601 tccccgacca ggcgctggaa ggtggcggga cgatcgaggt tcgacagcac gacgcgcgcg
      661 cgcagcgtct cgccgccctt gagcaccacg ccggtggcgc gtccgtcctc gagcagcacc
      721 tgatcgaccg tggcgccgag gcgaaccgtg ccgccaacgg cctcgatgct gcgcgccagc
      781 gcctcgctga gcgaacccat gccgcccttc acgcgccgca tcagcccgct gtcgccgctc
      841 tgcgcgaagg tatagaccag gcacagcgcg gagcccggcg tgtacggtcc cttgtaggtc
      901 gactggatcg cggcaaaggc cagcagcgag cggaaggtgt ggtgcttgcg cgggtccggg
      961 aagaagcggt cgatcacgtc catcgccgaa ccggtgaacg cgagccgcaa ctgttcgcgt
     1021 cgggcctcgt ccggagcccc gctcagcagc tcgtcgaggc tgcgcggcgt gcaaccgggg
     1081 ttgaaacggt ccatgacgct cgcggggtag gcgacgaact tcgcgaattt cgcgaagccc
     1141 gcgagcgcag cgggcccgtg gtggcgcagc aggtgccaca gctgccggcg cgccgagcgg
     1201 tagaacatga tcggcttgtg cccggggccg gcaaggttga tcgccatgac gggcagctcg
     1261 atgaactcca cgccgtggcg ctcgaactgc aggaagtcca gcacttcggg ggcgatcggg
     1321 aacagcgcgc tggcgccgac ctcgttgcgg cagcccggga agatctcccg cgtacccgcc
     1381 atgccgcccg cgtagcggtt cttctccagc acgagcacgc gccgcccgcg ctgggccagc
     1441 acccgcgcgg ccgccagccc gttgtggccg gcgccgatca cgatggcatc ccactggccg
     1501 tccatcgcac accccgctgt cgatgttttg tgttttgtgg tgcaaccggg caatcttgcg
     1561 gtcattttcc gcgagcgcgc aagcaggccc gatgtcgacc accgccgcga tcgtctccgt
     1621 gctaggcagc agcgggcgcc tgctggcgga tgccgtgctc gactggcacc cgcgaacgct
     1681 gccgcggcgt ggccgcgccg tggaggacgc cgcgttcctg aaccgcttgc tggccgagca
     1741 cggcaacgat gcgcgcagcc gcgcggcgcg ggccgcgacg gtgcgggtgc gcgcgacgcg
     1801 cccgccgagc agcaactgca gcaaccgcgt gctggaggtg gactgggcgc acggcgaggg
     1861 catgccgcgc acgctgttcc tcaagctccc gggcgaaccg ctcgcaaccc gcttcttctg
     1921 caatgcgatc cgcgtctggg aactggaatg cgcgttcttc cgctcgttcg cgcacgactt
     1981 cccgctgcgc ctgccccggg tgcatgcaat cgcgacgcgc ggcagccgtt tcgcgctgct
     2041 gcaggaggat ctcggcgccg atcccgcggt gcggctgttc acgaaccgcg cgatgctcga
     2101 gggggtgccg ctggagctgg cgcggcgctg catcgaggca ttcgccacgt ttcatgcgca
     2161 ctaccacggc ctggatgcgc aggcgcgcga gcgccggttg ccgctcgcgc ggcacccgtt
     2221 cctgtcgccg cacatgtccg cgatctcgca ggccatcaac cgtgcggcgg tggaaccctg
     2281 ccggcgcaag gcgcccgcgg tgttcggcgc agcgcaggcg gcacggtttc gtgccgcgat
     2341 ggggcgctgg caggacctgc aggcctggtg gtaccgcgag ccgctgacgc tggtgcacgg
     2401 cgacagccac atcggcaact tcttcgtcga cggcgagcgc atgggcatgc tcgatttcca
     2461 ggccgcgcac tggagcaagg gcatccgcga cctgcagtac ttcctggtga actcgatgcg
     2521 cgcggaagag ctcgcgtgcc acgagcgcgc gctggtttcg gagtacgccg ccgcgctgcg
     2581 tgcacgcggc gtcgcgttgg cggacgacga ggcctgggcg cagtaccgcg gcttcgcctt
     2641 ccagccgctg atgacgatcg tgacctcgct ggggctgggc ccgctgaccg agcgcgatgc
     2701 gctgatggaa gcggtgctgg cgcgcgcact cgcgctcatc gagcgcaccg ctttccacga
     2761 ctggctcgat gcgctgccgc gcgcagcctg aaccgatacc tgccgccagg agccccacgc
     2821 atgccccgcc ccgcgctgcc gctgcccgta cgcctgctga acaccggctt cgccgcgctg
     2881 gggcgcaccg gtccgcgact gctgccgctc gacccggaac gcctgatgga agccgcggcg
     2941 cgcaccgcgg ggctggacga cttcggctcg ccgtactttc gcgagccgct ggcacgtctg
     3001 tgcgattctc tggagcgcga ggcgcggctg acggcgctag ggcgcatgat cgcgcgccag
     3061 gacatcctgc ggctgctcgg caaccgcttg cgctgggtcg atatctccag gcgccacccg
     3121 gagatcggcg cggggcgcgt cgtcgcgccc gtgttcatcc tcggcatgcc gcgcaccggc
     3181 accaccagca tgcacgagct gctggcgctc gacccgcagt tccgcgtgcc gctcagctgg
     3241 gaggtggcac acccctttcc gccgccggag accgcgagct atcgcagcga cccgcgcatc
     3301 gcgcaggtgg atgcggagct ggcacgggtc gaccggctgc tgcccgagtt ccgcaacatg
     3361 cacccgatgg gcgccgagct gccgcaggag tgcgtggggc tgttcgcgca cgacttcgtg
     3421 tcgatgatct tcgacgtgca gttccggctg cccgcgtacc aggagtggct catccgcgag
     3481 gacatgggcg aggtgttccg caaccaccgc ggctggctgc agctgctgca gtggaagaaa
     3541 cccggcgaca cctgggtgct gaaatcgcca cagtacctat ggaacatcga ggacatgctg
     3601 cgcgagtacc cggacgcgcg cgtgatccag atgcaccgcg acccggtgac ggtggcgatg
     3661 tcggtgggca gcctgacggc cacgctgcgc ggcctggcct cggacacggt cgacctgaag
     3721 gaagtgacgc aacactacgc ggacctgctc gacttcggca cgcgccgcac gatggccgcg
     3781 cgtgcgagcg ggctgctggg cgccgggcgc gtgatcgacg tgcagttcgc ggactttcgg
     3841 gccgatgcac tggccgagat ccggcgcatc tacgcgcacc tcggcttgcg cctggacgat
     3901 gccgtcgccg aggcgatgcg gcgctttctc gatgcgggca gggcgagcgc tcgccacgcg
     3961 cgccaccgct acgacctggc ggcctcgggc atcgacctgg cgcgcgagcg ggagcgcttc
     4021 gccgactatc agcaggccta cggcatcggt tcggagatct cgggcggctc gtaccagaac
     4081 ggcgaggaat acgcgcgctc ctgcagcacc tgaggcgcgg gcgcctcgac cttcagcttc
     4141 gcggcgtcgt gggtgctcca gcgcggcgtc gggatctcga gcacgcgcac gtagtagaac
     4201 gcgcgtgtcg ccggatcgaa ctcgggatct tcccacagca cctcgagcgc ggcggcgccg
     4261 atggtgttcg tgtagctcgc cgcggccaca tcgactgtcg agcccacggg aggcagcttg
     4321 ccggtggcgg ggtcgggcac gcgatcgccg ctccaggcga cctcgaagat cttctcctgg
     4381 ccctggccgc ctgcgtcgag ccagcccttg atgacctgca cgcggtcgag gttggcgccg
     4441 tcggggtcct tggccgcgcg gatcagcagg cgcggcgcgc cggcgtccgc cgggcgtgcg
     4501 ggcaggtcgc cgcccatcgg cacgccgccc gcgtaggcct tgtcgaggta ggcgaggtcg
     4561 ccgagcagcg cctgttcgta gccccagccc ccgaagacgc gaaggccgat gcgcggcccc
     4621 gaggtcgcgt aggtctcgcc gcgacgcagc gcgtcgaaga tcgcctcgcg cgtgttaccg
     4681 gtggcccaca cggccgcgag gccctgcgcg ctcatgatcc gaccgagtcc gcggtgcagt
     4741 tcggaggccg gcttgatgtc gaggcgctgc tcgggcgtac cgtcgatgag cggcagcttg
     4801 ccgtggtagt tgtcttcctc gatcggcgag gtggagttgt gcgaatcgct cgagccgatg
     4861 aacgagaagt ggtacgggtt gccgatgccg ctggcgtcga gctcgaggcc caccccgagg
     4921 gcctcgcgca cgtagctgcc ggcgggctgc gagatcgtcg gctccgggcc catcttcaac
     4981 gtgatcggga agatctcgaa gttcgcgaac tcgtcgttcg gcgcgagcac ggggtgcact
     5041 tccgactggc ccttgatctg gatgatctcg tgcacgggct cgttgcgcat gcgcctgctg
     5101 acgtagtcgg cgtcgaaggg gcgaccgtcg aaggtgcgcg tgccgaacat cagcccgttg
     5161 ctcgcattct ggttgtgcgg tatcgcgagg ctgtcgatgc cggcggcgcg cttctcctcg
     5221 agccaggccc agagctgctc cgggtccggc gagtcgaaac tgctgaacgg ccgctcgggc
     5281 acggtgtcgc cgcgaaagat cacgttgcga tggaggttgt tgccgtcgat ggtgctggtc
     5341 cactcgtagc cgaccagcgc ggtgaaggcg cccgggtcgt tgtggcggtt ggccgcggcc
     5401 acgatctgct gccagacgct ggaggtcacc tgcgggctca cgagttccgc gatgggctcg
     5461 ccgctgttca gcgttccgac cacttccgtg agcgcggtct gtgcttcgcc cgggtctgcc
     5521 gacatgaagc gcgccgccag cggcagcttc gacagcgggc tctgcggatc ggcgagctgc
     5581 ggcagcacgc ccatgtattc cgaatggtcc gtgaccgcgg cgaaatcgag cgggcggtgc
     5641 agttgcaccg gataaccggc cgcgtgcagc gtctgctccc ccttcgcgaa gcggtaggcg
     5701 tcgtcgggta gcgtgcggtt gccgaccgcg aaggcatcgg gcgagaagct ggtgtggatg
     5761 tgcaggtcgc cgaacagcac gcgctcggga tggggcggcg ctaccggctg cgccggggcc
     5821 tcgacaggcg tcgccgcggg cacggcgccc ggctcggttg ccgccggctg ctccggggca
     5881 tcgcaaccgc cgaggagcag ggtgaacagg aggggcgctt tcaggcttct catgtggact
     5941 ctccgtgtgt cagcctgcgg gatgatcgca attcagcggc agttcaggca attcggaatt
     6001 gcgtacgtcg accccgcttg cgctgcagga tttgacacgc atcaacgcga ccggaccgtc
     6061 ttcgggaaca ttatgaacgc ttttcccgcc accatgaagg aacgccatga cccgggatcg
     6121 aaacccatcg caaagcggca cccaagaagc caccacgccc ggacatcggc cgcgcgcggt
     6181 gcagtccggt gacgtgccac cgtccatgca acccgctgcg ctggagcggc accaggccga
     6241 tcgcgcgctg gaacgcgcgt acacggtaca gtcgatggtg gtgaccgacg cgctgagcgc
     6301 tgcgagcgcg cacgagccgg cggtgctggc cgaggtgcat gcgatgctcg acggcgtgtc
     6361 gccccaggat gcgcgcgcgc tgcgccgcat gctggcgcag cgcctggccg tcaccggcga
     6421 caccgcgagc gatcccgaca ccgcgctagc ccccaactgg cgggacggcg catatccgta
     6481 ccggaatctg ctttcgcggc gcagttacga acgccaaaag taccggctgc aggtcgaact
     6541 gctgaagctg caggcctggg tcaaagacac aggtgcccgc gtcgtgatcc tgttcgaggg
     6601 ccgcgacgcg gccggcaagg gtggcgcgat aaagcgcttc atggagcacc tgaaccctcg
     6661 cggcgcgcgc gttgtcgcgc tcgagaagcc cagcgagatc gagcgcggcc agtggtactt
     6721 ccagcgctac atcgagcatc tgcccacgcg aggcgagatc gtgctgttcg accgcagctg
     6781 gtataaccgc tccggcgtcg aacgcgtgat ggacttctgc accgaaggtg agtacgacga
     6841 gttcctgcgt caatgcccgg agttcgaacg tcagctcgta cgttccggaa tccacctctt
     6901 caagttctgg ttctctgtca gccgcaagga gcagcggcgc cgcttcaagg agcgcaagct
     6961 gcatccgctg aagcagtgga agctcagccc ggtcgacctg gcgtcattgg acaagtggga
     7021 tgactacacg ctcgcgaagg aggcaatgtt cctgcactgc gacacctccg acgcgccgtg
     7081 gacggtgatc aagagcgatt gcaagaagcg cgcgcggctg aatgcgatgc gctatgtcct
     7141 gcaccgcatg ccgtatacgc gcaaggatcc gtcgttgctg ggcccggtgg acccgctgat
     7201 agtcggccgg ccgtcgctgg tgccaagcgt cggcgacgac cagctcgccg ggagctgagc
     7261 gccggcggcc gggacggccg atccggcgtc tggcaccgac gcgtgcgtgc ccgtctcaat
     7321 ggtccgccgg cttggtcccg ttcatgatgc ggtcagtcca cgcgatgccg atcgccgaca
     7381 ggatgaatac cgtatggatc acggtctgca ggatgatgcc ttcctgggtg gccgtggcac
     7441 agcgcacgcc cgcttccacc gtcgagcaca gcgggtacgc gcccaccatg ccggcttcga
     7501 tgaaggtctt cagcaggtgg atggaggaaa tgccgatgat ggccatcgcg agcttcacct
     7561 tgagcaccga ggcgttcacg tggctcagcc attccggctg gtcggggtgc ttctccaggc
     7621 gcaggcgcga cacgaaggtc tcgtagccgc ccacgatgac catcatcagc aggttggaga
     7681 tcatcaccac gtcgatcagc gccagcacga tcagcatcac ctgctgctcg ttcatctcga
     7741 atgcgccgtg caccaggtgc agcaactctt tcacgaacag caccacatag acgccctggg
     7801 cgacaatcag gcccaggtag agcggcagct gcagccagcg cgaggaaaag atcaggctgg
     7861 gcaatggctc cagccggtaa tcgtttgatg tatcggtgtc gtgctcgggc tttttcatgc
     7921 gctgggatca agggatgcga agacgtaatt ctaaaccagc tccatgaaca cctgacgagg
     7981 tatttttgcc acgtgcgagc agaagatccc gagacgccat ccaggcctca aaacgagacc
     8041 gcgatgccca tgcccggacc gtgctggcgc aggtcgtagc gctggtagtc gcgaccgctc
     8101 ccgtcctcgt agtcgtaagc gatcgcccgg taaccgacgt aaaaactgaa gcgctccgag
     8161 ctctggtacg tgaacttcgt cagtgcgtgc cag
//
LOCUS       DAORUQ010000143         8160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9795_length_8160_cov_6.222455, whole genome shotgun sequence.
ACCESSION   DAORUQ010000143 DAORUQ010000000
VERSION     DAORUQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_8160_cov_6.222455"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..573
                     /locus_tag="PLW13_11865"
     CDS             58..573
                     /locus_tag="PLW13_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQY71119.1"
                     /translation="MATAPVPATATEREARLGQMAGLDEIDRRLIELLRGDGRMAFRA
                     LADTLGVTESAVRARVRQLETSGAMKVVAVADAEAVGYELLLAVGVEVDGRPALAVAE
                     ALAGIPEVFSVSLVVGTHDIELLMVARDHEHLAELVTDRLVNIPGVRRLLTGLAVDVL
                     KNQPDWVPFDD"
     gene            566..1069
                     /locus_tag="PLW13_11870"
     CDS             566..1069
                     /locus_tag="PLW13_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQY71120.1"
                     /translation="MTKRRFDEIDQRILDYLSRDARTSNRRIADELRVTEGTVRARIK
                     RMEDEGQIRITAITNIRKLANPALAFVWIEVDRGSQGREVAAALARHPQIGFVGIMVG
                     RFDILAITMVQSAEQLSEFLHSTVHSIPGVRHTECSLGARFVKHDYRLSRIVTQPRTY
                     EAESENA"
     gene            1216..2094
                     /locus_tag="PLW13_11875"
     CDS             1216..2094
                     /locus_tag="PLW13_11875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQY71121.1"
                     /translation="MNKLMSAGDVVGQLASGMTIGIGGWGPRRKPMALVREILRSDLK
                     DLTVVAYGGADVGMLCAAGKVRKVVFAFVSLDFIPLEPYFRLARQKGELEIMEIDEGM
                     YLLGLRAAAMRVPFLPTKIGLGTDVLRHNPQIKTIVSPYADGRELVAMPAIELDAALI
                     HADRADARGVCQIKGPDHYMDDVMARAAGKTYVSCDELVDSSFFASPEEARYVFWERS
                     ETTGVVHIPGGAHPSSCSPLYGFDVPHFKEYQASAGQENGWSDYVQKYVGDSEAYYQQ
                     QVGGVEAIRKLPLPTY"
     gene            2112..2909
                     /locus_tag="PLW13_11880"
     CDS             2112..2909
                     /locus_tag="PLW13_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HQY71122.1"
                     /translation="MAQEYTLAELMICAASEAWRNDGEVLATGIGTLPRLAASLSMLS
                     TNPDLMMTDSEAFLLSAPNPIGPRAADFKPAYETWMGFSRIFDNVWGGKRHAMVGPTQ
                     IDRFGQANISCIGDYARPKAMMLGVRGFPGNSISHANSFFVPGHNTRVFKDGECDVVA
                     SIGYNPARLAKGYSLDEVDIRLVITDLCVMDFGGPGHQIRLRSLHPGITVEQVQQNTG
                     FPVFVPEDVITTPAPTAEQLEIIRRMDPHNLRARELKDNPPGDRTAR"
     gene            2926..3993
                     /locus_tag="PLW13_11885"
     CDS             2926..3993
                     /locus_tag="PLW13_11885"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY71123.1"
                     /translation="MLQTRLTELLGCRYPVVQTAMGWVADPKLVAATCNAGGFGFLAG
                     ATIPAEQMERDILRTKELTQQPFGVNFHMYQPNAADIVGLVVKHGVRAVSYSRSPGKE
                     FIRKLKDAGVVCMPTVGAVKHAVKAVELGADVVTVQGGEGGGHTGSVPTTLLLPEVVR
                     GVKVPVVAAGGFKDGRGLVAAMAWGAEGIAMGTRFLMTADSPVPQATKERYLACRNPA
                     DIIVSTAMDGMPQRMVMNEILAELERAGPLKKLLIALRNGLAFRKHSGATISQLLQSA
                     IAMQREDGLTPAQAIMSANAPMIIQRAMVEGRPAEGVLPSGQVAGVIDDLPSCAELIA
                     RIAAEAEECLAALAKRAGPAA"
     gene            4492..5250
                     /locus_tag="PLW13_11890"
     CDS             4492..5250
                     /locus_tag="PLW13_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HQY71124.1"
                     /translation="MSKPFKTTISEGIAELVFDHPPVNAFDSAGWAAIAAEIEALGGN
                     DDVNVIIIAAEGRGFCAGVDIKELAADRNLIVKVNKGNYDTFKAVHLNPKPVIVAVHG
                     FVLGGGIGISGAADIVVASECARFGVPEVDRGAMGGGAHLQRLFPVQKVRYMYFTGEM
                     IDAQEAYRLGAIERVVPRDQLRATARDIAAKIAAKSPAMIRLAKEALNGIEDGNLEDK
                     YRSEQGFTLEAYTTKDSQEARDAFVQKRDAKFDG"
     gene            5278..6444
                     /locus_tag="PLW13_11895"
     CDS             5278..6444
                     /locus_tag="PLW13_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY71125.1"
                     /translation="MDLAYTEKQKAFRREVREWLAANVPPEPLASFDTAEGFRQHRAW
                     EAKLNSGRWGMVTWPAELGGRGCDLVEWLIFEEEYWRAGAPLRVNQNGIFLLGPTLME
                     YGTAEQKKRFLPKMATGEEVWCQGWSEPGAGSDMAAIRARAERQGDHYVINGQKTWST
                     RAVWADWCFGMFRTDPASERHHGLTFILVPLNLEGITVRPIPQLDGLPGFAEIFFDNV
                     KVPVDCRLGDEGKGWKVAMATAGFERGLMLRSPARFQQTAARLVQLYKANRASADRDP
                     TVRDAVLRAWMDAEAYCLTTYQTASRLNAGGRIGEESSTNKIFWSELDQRMHETALSI
                     LGARAELLPHAPDAGGVGNWLDGFLFAQSGSIYAGTNEIQRNIIAQRMLGMPRA"
     gene            6456..7505
                     /locus_tag="PLW13_11900"
     CDS             6456..7505
                     /locus_tag="PLW13_11900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71126.1"
                     /translation="MDFRFSDDQKLFQQTVREFLVAEITPERIRASWQTDTGRSDALW
                     AQLAELGLTGITVPEAHGGLGMNELDFVLLAEEIGYVALPEPLIDTVLVGVPCLRDCG
                     NEQLAAEWLPKIAAGEAKLAIGLDCNPLVADAHVAGLLLLAHGDELHAVPRADVTLTA
                     NECLDPSRRLFRVDWTPSAVTRLAGGAAGRTLIDAALNRAALGAAAQCLGLAQRMIEI
                     SVAYTRDRQQFGQAIGTFQAVQHLMANVAVKLEYARAPVHRAAYDIAHGNPRASVSVS
                     HAKIAATEAALLGARNSIQVHGAMGYTWEVDLHLFAKRAWTLDSAWGDRAFHKQRVAD
                     FVLADNAAIGAGATF"
     gene            7521..>8160
                     /locus_tag="PLW13_11905"
     CDS             7521..>8160
                     /locus_tag="PLW13_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQY71127.1"
                     /translation="MAEAYIVDALRSPTGKRKGSLAAVHGADLGAHVIRAIVERNAIP
                     AEDYDDVIFGCVDTIGALAGDIARTAWLAAGMPLHVPGTTVDRQCGSSQQAVHFAAQA
                     VMSGTQDVVLAGGVQTMSSIPISSAMLAGQPLGFTTPFAESLGWQKRFGDAPVNQFYA
                     AQRIADHWGASREAMEVFAKESHDRALKAIAEGRFDREIVPYTLPDGTVFRMD"
BASE COUNT         1372 a         2707 c         2855 g         1226 t
ORIGIN      
        1 gctcgcaaat cgacggtctc gcgacggccg tcgaaagatt cacaggctct gaggccgatg
       61 gccacggcgc cggtaccggc aaccgcgacc gagcgcgagg cgcgcctcgg ccagatggcg
      121 ggcctcgacg agatcgatcg ccgcctgatc gagttgctgc gcggcgatgg ccgcatggcg
      181 ttccgcgcgc tggccgacac gctgggcgtc accgagtcgg cggtgcgggc gcgggtgcgc
      241 cagctcgaga cttccggcgc gatgaaggtc gtcgcggtcg ccgacgccga agcggtcggc
      301 tacgagttgc tgctcgcggt cggcgtcgag gtcgacgggc ggcccgcgct cgcggtggcc
      361 gaggcgctgg ccggcattcc cgaggtgttc tcggtgagcc tggtcgtggg cacgcacgat
      421 atcgagttgc tgatggtggc gcgcgaccac gagcacctcg ccgaactggt cacggaccgg
      481 ctggtgaaca tcccgggggt gcgccgcctt ctcaccgggc tcgccgtcga cgtgctcaag
      541 aaccagcccg actgggtgcc cttcgatgac taaacggcgt ttcgacgaga tcgaccagcg
      601 catcctcgat tacctctcgc gtgacgcgcg caccagcaac cggcgcatcg ccgacgaact
      661 gcgcgtcacc gagggcaccg tgcgcgcgcg catcaagcgc atggaggacg aggggcagat
      721 ccgcatcacc gcgatcacca atatccgcaa gctcgccaat ccggcgctcg ccttcgtctg
      781 gatcgaggtc gatcgcggta gccagggccg cgaggttgcc gccgcgctgg cgcgccatcc
      841 gcagatcggc tttgtcggca tcatggtggg gcgtttcgac atcctggcga tcaccatggt
      901 gcagtcggcc gagcagctga gcgagttcct gcacagcacg gtgcactcga taccgggcgt
      961 gcgccacacc gagtgctcgc tgggcgcgcg tttcgtgaag cacgactacc gcctcagccg
     1021 catcgtgacg cagccgcgca cgtacgaggc tgaaagtgaa aatgcgtagc agactggatt
     1081 ccagcgcggg aattccggac ttgtcggctt gatatgaatc attatgcgta gaattcggcc
     1141 agatatttta ctgttttcgg ttgaatgagt ttatactgcc ggcccggtga tcggccaagg
     1201 cgaggaagct tcacgatgaa caagctgatg agtgcgggag atgtggtcgg gcagctcgcg
     1261 agcggcatga ccatcggcat cggcggctgg gggccgcgtc gcaagccaat ggcgctggtg
     1321 cgcgagatcc tgcgatccga cctgaaggat ctcacggtgg ttgcctatgg cggcgccgac
     1381 gtggggatgc tgtgcgcggc cggcaaggtg cgcaaggtgg tattcgcctt cgtgtcgctc
     1441 gacttcatcc cgctcgagcc gtacttccgc ctcgcgcggc aaaagggcga gctcgagatc
     1501 atggagatcg acgagggcat gtatctgctg ggcctgcgcg ccgccgcgat gcgcgtgccc
     1561 ttcctgccga ccaagatcgg gcttggcacc gacgtgctgc gccacaaccc gcagatcaag
     1621 accatcgtct cgccctacgc cgacggccgc gagctggtcg cgatgccggc gatcgagctg
     1681 gacgccgcgt tgatccacgc cgaccgcgcc gacgcgcgcg gcgtctgcca gatcaagggc
     1741 ccggatcact acatggacga cgtgatggcg cgcgcggcgg gcaagactta cgtcagctgc
     1801 gacgagctgg tcgactcgag cttcttcgcc tcgcccgagg aggcgcgcta cgtgttctgg
     1861 gagcgctccg agaccaccgg cgtggtgcac atccccggcg gcgcgcaccc gagcagctgc
     1921 agcccgctgt atggcttcga cgtgccgcac ttcaaggaat accaggcatc tgccgggcag
     1981 gagaacggct ggagcgacta cgtgcagaaa tacgtgggcg attcggaggc ctactaccag
     2041 cagcaggtcg gcggagtcga ggcgatccgc aagctgccgc tcccgacgta ctgagcaaga
     2101 ggtcaccgac gatggcacag gaatacactc tggcggaact gatgatctgc gcggcctccg
     2161 aggcctggcg caacgacggc gaggtgctcg ccaccggcat cggcacgctg ccgcggcttg
     2221 ccgcttcgct gagcatgctg agcacgaacc ccgacctgat gatgacggac tcggaagcct
     2281 tcctgctgtc ggcgccgaac cccatcggtc cgcgcgcggc ggatttcaag ccggcctacg
     2341 agacctggat gggcttctcg cgcatcttcg acaacgtctg gggcggcaag cgccacgcga
     2401 tggtcgggcc gacgcagatc gatcgcttcg gccaggccaa catctcgtgc atcggcgact
     2461 acgccaggcc caaggcgatg atgctgggcg tgcgcggctt cccgggcaac tcgatcagcc
     2521 acgccaattc cttcttcgtg cccggtcaca acacgcgcgt gttcaaggat ggcgagtgcg
     2581 acgtggtggc ctcgatcggc tacaacccgg cgcgcctggc caagggctac agcctcgacg
     2641 aggtcgacat ccgcctggtg atcaccgatc tgtgcgtgat ggacttcggc ggccccgggc
     2701 accagatccg gctgcgctcg ctgcaccccg gcatcacggt ggagcaggtg cagcagaaca
     2761 ccggcttccc cgtgttcgtg cccgaggacg tgatcaccac gccggcgccc accgcggagc
     2821 agctcgagat catccgccgc atggacccgc acaacctgcg cgcgcgcgaa ctcaaggaca
     2881 acccgcccgg cgatcgcacc gcgcgctgaa ccaggacccg cgatcatgct gcaaacccgc
     2941 ctcaccgagc tgctcggctg ccgctacccc gtggtgcaga ccgcgatggg ctgggtagcc
     3001 gacccgaagc tggtcgccgc gacctgcaac gcgggcggct tcggtttcct cgccggtgcc
     3061 acgatcccgg ccgagcagat ggagcgcgac atcctgcgca cgaaggagct tacgcagcag
     3121 cccttcggcg tgaacttcca catgtaccag ccgaacgcgg cggacatcgt cgggctcgtg
     3181 gtgaaacacg gcgtgcgcgc cgtgagctac agccgctcgc cgggcaagga gttcatccgc
     3241 aagctgaagg atgccggggt ggtgtgcatg ccgacggtgg gcgcggtgaa gcacgcggtg
     3301 aaggccgtgg aactcggcgc cgacgtggtg accgtgcagg gcggcgaagg cgggggccac
     3361 accggctcgg tgcccaccac gctgctgctg cccgaggtgg tgcgcggcgt gaaggtgccg
     3421 gtggtcgctg cgggtggatt caaggacggc cgcggtctgg tcgcggccat ggcctggggt
     3481 gccgagggca tcgcgatggg cacgcgcttc ctgatgaccg ccgacagccc ggtgcctcag
     3541 gcgaccaagg aacgctacct cgcgtgcagg aacccggccg acatcattgt ctcgaccgcg
     3601 atggacggga tgccgcagcg catggtcatg aacgagattc tcgccgagct cgagcgcgcc
     3661 gggccgctga agaagctgct gatcgcgctg cgcaacgggc tggccttccg caagcacagc
     3721 ggtgcgacga tttcgcagtt gctgcaatcc gcgatcgcga tgcagcgcga ggacgggctg
     3781 acccccgcgc aggcgatcat gtcggccaat gcgccgatga tcatccagcg cgcgatggtc
     3841 gagggcaggc ccgccgaggg cgtgctgccg agcgggcagg tggccggcgt gatcgacgat
     3901 ctgccgagct gcgccgagct gatagcacgt atcgccgccg aggccgagga gtgcctggct
     3961 gcgctggcga agcgggcggg cccggcggct tgaattgttc gttcgcaccc gttgcgttgc
     4021 aaggcgtgtg gcatcggcgc tgagggagcg tgggcgcggg agcgtttatg tctgctggga
     4081 cacgccgtga atacgtccct gtaggctcga ctgcggcatc cctgccgcag acggtccccg
     4141 cagccacaaa cgctcccgcg cctcggagac gcagtgccag ttgcaggcat ccagaggcac
     4201 aaggcctgac tggtatgtcg aagttgatgt gatcgcattc ccgtcccggt gggtcgatgt
     4261 gttcgggccg cgacgacttt tggaacgagg aattgaattg gtgccgggtg ccaccggcgt
     4321 cgaggtcgga gaagggggtg cagtgtgacg ggaccgtctg tcgcatggat gcgacagtcg
     4381 agcctacagg gatgtattca cggcgtgtcc cgacacactg cacccccttc tccggccgca
     4441 tcaggcccgc cgcactagca agcggcgaac gatcaccgca ggagaaatgc aatgagcaaa
     4501 ccattcaaga ccacgatcag cgagggcatc gcggagctgg tgttcgacca cccgccggtg
     4561 aatgcgttcg acagcgcggg ctgggcggcg atcgcggcgg agatcgaggc gctgggcggc
     4621 aacgacgacg tcaacgtcat catcatcgcg gccgaggggc gcggcttctg cgcgggcgtg
     4681 gacatcaagg agctcgcggc cgatcgcaac ctgatcgtga aggtgaacaa gggcaactac
     4741 gacaccttca aggcggtgca cctgaatccc aagccggtga tcgtcgccgt gcacggcttc
     4801 gtgctgggtg gcggcatcgg catcagcggc gcggcggaca tcgtggttgc atccgaatgc
     4861 gcacgcttcg gcgtacccga ggtggaccgc ggcgcgatgg gtggtggcgc gcacctgcag
     4921 cggctgttcc cggtgcagaa ggtgcgctac atgtacttca ccggcgagat gatcgacgcg
     4981 caggaggcgt accgtctcgg cgccatcgag cgcgtggtgc cgcgcgatca gctgcgcgcc
     5041 acggcgcgcg atatcgccgc gaagatcgcc gcgaagagcc cggcgatgat ccggctcgcg
     5101 aaggaagcgc tgaacggcat cgaggacggc aacctcgagg acaagtaccg cagcgaacag
     5161 ggcttcacgc tggaggcgta cacgaccaag gactcgcagg aagcgcgcga tgccttcgtg
     5221 cagaagcgcg acgcgaagtt cgacggctga caggcaaaac ccggagaatc gagcatcatg
     5281 gacctggcat acaccgagaa gcagaaggcc ttccggcgcg aggtgcgcga atggctggcc
     5341 gccaacgtgc cgcccgagcc gcttgcgagc ttcgacacgg ccgagggctt tcgccagcac
     5401 cgcgcatggg aagcgaagct gaacagcggc cgctggggca tggtgacctg gcccgcggaa
     5461 ctcggcgggc gcggctgcga cctcgtcgag tggctgatct tcgaggaaga gtactggcgc
     5521 gcgggcgctc cgttgcgcgt gaaccagaac ggcatcttcc tgctcggccc cacgctgatg
     5581 gaatacggca cggcggagca gaagaagcgc ttcctgccga agatggcgac cggcgaggag
     5641 gtgtggtgcc agggctggtc cgagccgggc gccggctccg acatggcggc gatccgcgcg
     5701 cgcgccgagc gccaggggga tcactacgtg atcaacggcc agaagacctg gtcgacgcgc
     5761 gcggtgtggg ccgactggtg cttcggcatg ttccgcaccg atcccgcctc cgagcgccac
     5821 cacggcctca cgttcatcct ggtgccgctg aacctcgagg gcatcacggt gcgcccgatc
     5881 ccgcagctcg acggcctgcc cggtttcgcg gagatcttct tcgacaacgt gaaggtgccg
     5941 gtggactgcc gcctcggcga cgagggcaag ggctggaagg tggcgatggc caccgccggc
     6001 ttcgagcgcg gcctgatgct gcgcagcccg gcgcgattcc agcagaccgc cgcgcgcctg
     6061 gtgcagctct acaaggccaa ccgtgccagc gccgaccgcg accccacggt gcgtgacgcg
     6121 gtgctgcgcg cgtggatgga tgccgaggcc tattgcctca cgacctacca gaccgcgagc
     6181 cgcctgaacg ccggcggcag gatcggcgag gagtcgagca ccaacaagat cttctggtcc
     6241 gagctcgacc agcgcatgca cgagaccgcg ctcagcatcc tcggcgcgcg tgccgagctg
     6301 ctgccgcacg cgccggatgc gggcggcgtg ggtaactggc tcgacggttt cctgttcgcg
     6361 cagtcgggca gcatctacgc cggcaccaac gagatccagc gcaacatcat tgcccagcgc
     6421 atgctcggca tgccgcgcgc gtaaggggaa cgaacatgga tttccgcttc agcgacgacc
     6481 agaaactgtt ccagcagacg gtgcgcgagt tcctcgtggc cgagatcacg cccgagcgca
     6541 tccgcgcgtc atggcaaacg gacaccgggc gctcggatgc cctgtgggcg cagctcgccg
     6601 agctcggact caccggcatc acggtgcccg aggcgcacgg cggcctcggc atgaacgagc
     6661 tcgacttcgt gctgctcgcc gaggagatcg gctacgtggc gctgcccgag ccgttgatcg
     6721 acaccgtgct ggtgggcgtg ccctgcctgc gcgactgcgg caacgagcag cttgccgccg
     6781 agtggctgcc gaagatcgcc gccggcgagg cgaagctcgc gatcggcctc gactgcaacc
     6841 cgctggtggc cgacgcgcac gtggccgggc tgctgctgct cgcgcacggc gatgagctgc
     6901 acgcggtgcc gcgcgccgac gtcacgctga ccgccaacga gtgtctcgat ccctcgcgcc
     6961 ggctgttccg cgtcgactgg acgccctcgg ccgtcacgcg gctcgccggc ggcgccgccg
     7021 gccgcacgct gatcgacgcc gcgctgaacc gcgcggcgct cggcgccgcg gcgcagtgtc
     7081 tcgggctcgc gcagcgcatg atcgagatct cggtggccta cacgcgcgat cgccagcagt
     7141 tcggccaggc gatcggcacc ttccaggcgg tacagcacct gatggcgaac gtggccgtga
     7201 agctcgagta cgccagggcg ccggtgcacc gcgccgccta cgacatcgcg cacggcaacc
     7261 cccgcgccag cgtgtcggtc tcgcacgcga agatcgccgc gaccgaggcc gcgctgctcg
     7321 gcgcgcgcaa cagcatccag gtgcacgggg cgatgggcta cacgtgggag gtggacctgc
     7381 acctcttcgc caagcgcgcg tggacgctgg acagcgcctg gggcgatcga gccttccaca
     7441 agcagcgcgt ggcggatttc gtgctcgccg acaatgccgc gatcggcgcc ggcgcaacat
     7501 tctgaacgga ggatctgacc atggctgaag cctatatcgt cgacgcgctg cgctcgccca
     7561 ccggcaagcg caagggctcg ctggccgccg tgcacggcgc cgatctcggg gcgcacgtga
     7621 tcagggccat cgtggagcgc aacgcgatcc cggccgagga ctacgacgac gtgatcttcg
     7681 gctgcgtgga cacgatcggc gctctggccg gcgacatcgc gcgcaccgcg tggctcgccg
     7741 ccggcatgcc gctgcacgtg cccggcacca cggtcgatcg ccagtgcggc agctcgcagc
     7801 aggcggtgca tttcgcggcg caggccgtga tgagcggcac ccaggacgtg gtgctcgccg
     7861 gcggcgtgca gacgatgagc agcatcccga tttcctcggc catgctcgcc ggccagccgc
     7921 tcggcttcac cacgcccttc gccgagagcc tgggctggca gaagcgcttc ggcgatgcgc
     7981 cggtcaacca gttctatgcc gcgcagcgca tcgccgatca ctggggcgcc agccgcgagg
     8041 cgatggaagt cttcgccaag gagagccacg accgcgcgct gaaggcgatc gccgagggcc
     8101 gcttcgaccg cgagatcgtg ccctacacgc tgcccgacgg cacggtgttc cgcatggacg
//
LOCUS       DAORUQ010000144         8156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9802_length_8156_cov_7.026046, whole genome shotgun sequence.
ACCESSION   DAORUQ010000144 DAORUQ010000000
VERSION     DAORUQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9802_length_8156_cov_7.026046"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="epmA"
                     /locus_tag="PLW13_11910"
     CDS             <1..858
                     /gene="epmA"
                     /locus_tag="PLW13_11910"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HQY71128.1"
                     /translation="EVDTPQLCPVTATDPLLESIPALPFGDDSPWFLQTSPEFAMKRL
                     LAAGSGPIYQMAHAFRRRESGPRHNPEFTMLEWYRPGFDTAALLAEVAQLVGEVIGAR
                     PTRRDAWGALFLRHAGLDPFAASDAALAERAAAIAGEAARGWPRDELLDLLFSELVEP
                     RLGHDCLQFVDAFPASRASLARTTLDAAGNPVAERFELFIDGYEIANGYNELCDADEL
                     RRRMDADNRARAARGLPPSPPDERLLAAMASGLPECAGVALGVDRLIMIALGAQHIGE
                     VMAFSAERA"
     gene            complement(1377..2231)
                     /gene="asd"
                     /locus_tag="PLW13_11915"
     CDS             complement(1377..2231)
                     /gene="asd"
                     /locus_tag="PLW13_11915"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459167.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HQY71129.1"
                     /translation="MRERLFIAMQHLLPQHLLSRLTGMLASSGFQPLKDWLIRRFIAQ
                     FAVDMSEAAEPDPGAYPHFNAFFTRALREGMRPVDRAPQAITSPADGAVSQLGAIEDG
                     RMFQAKGHWFSAAELLADPADARLFANGSFATIYLSPRDYHRVHMPLGARLRTLRYVP
                     GKLFSVNQVTAESVPGLFARNERLVCLFDTEHGPLAMVLVGAMVVAGIETVWTGPVTP
                     AGRQVLNVDYELDAAARTLEKGAEMGRFQLGSTVILLLPPGVASWDEALAAGSPLRMG
                     QRIGSLSN"
     gene            complement(2249..3226)
                     /gene="rsgA"
                     /locus_tag="PLW13_11920"
     CDS             complement(2249..3226)
                     /gene="rsgA"
                     /locus_tag="PLW13_11920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HQY71130.1"
                     /translation="MAGRKLSQNQARRVEKLQRRRTEHAASEPDPDSPALDGVVISRF
                     GKRVDVEDPRAAGTVCRCHMRSNIDSLVAGDRVVWHRDGEGGIVTARHERATVLSRPD
                     MHGTLRPAAANLDRIVIVIAPEPPPHANLLDRYLAAAEDVGLQALILLNKTDLLTDPA
                     ALEPLLGVYRAIGYAVLHASAHAGGLDELQAALAGHTTAFVGQSGVGKSSLISALLPH
                     EQDIRIGELSHAVTKGRHTTTAARLYHLPAGGDLIDSPGIREFSLAHLDAARTAHGFV
                     EFRPHLGRCRFRDCVHRNEPGCALREAVAAGTVSAARYASFLQITGEPS"
     gene            3360..3908
                     /gene="orn"
                     /locus_tag="PLW13_11925"
     CDS             3360..3908
                     /gene="orn"
                     /locus_tag="PLW13_11925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HQY71131.1"
                     /translation="MMLEDGLVWVDLEMTGLDPDRDVVIEIATIVTDAHLNVIAEGPV
                     FAIHQGDAVLATMDEWNTRQHGQSGLTQRVRESRLDEEAVQDLTLEFLRRHVPPGKSP
                     MCGNSICQDRRFMARRMPRLEAFFHYRNLDVSTVKELARRWRPDVLATVTKQGAHLAL
                     DDIRDSIAELRHYREHFFRMTP"
     gene            complement(4220..5308)
                     /gene="queG"
                     /locus_tag="PLW13_11930"
     CDS             complement(4220..5308)
                     /gene="queG"
                     /locus_tag="PLW13_11930"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQY71132.1"
                     /translation="MITTDSAREDATGSLAALAVDIRAWARELGFPSLGICGTDLSPH
                     DERLREWVAQGLHGELDYMTRHGARRWRPEELLPGTLRVISVRMDYHRADSAEPSGIL
                     NDAERAYVSRYALGRDYHKLMRARLARLADRILAARPGSAQRVFVDSAPVLERGVAQK
                     AGLGWIGKNTLLIDPRAGSWFFLGEIYTDIPLPLDAEFSGMHCGSCTACLDVCPTAAF
                     VSPFVLDARRCISYLTIELQGAIPQDLRPLIGNRVFGCDDCQLVCPWNKFARPSCEAD
                     FAPRHGLDAATLTELFAWSAEQFAERAAGTPLYRLGHERWLRNLAVALGNGPATDAAR
                     AALLARSEHPSELVREHVTWALEQLATQ"
     gene            5423..6952
                     /locus_tag="PLW13_11935"
     CDS             5423..6952
                     /locus_tag="PLW13_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019582198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQY71133.1"
                     /translation="MPDAELQPALYTAAETRALDRAAIDGAGIPGIVLMSRAGRAVFE
                     LTCARYPGVLPVHVVCGAGNNGGDGFIVARLARERGLAVTVSLLGEPARIAGDARLAL
                     EAALAAGVQVRPFTPQLRFDAGVIVDALLGTGLGGPVREAQAQAIAAMNASGLPIVAV
                     DIPSGLCSDTGCVRGVAVRAAHAVTFIGRKRGLYTAAARDHCGELHFDALGVPAQTYD
                     AVPAAAELLSLEDLLAALAPRARAAHKGSFGHVLVIGGGRGMAGAAAMAAESAARCGA
                     GLVSLATWPGNVAATLARRPEIMAHGVEHGHELEPLLARAGVLVIGPGLGRGPWAQQL
                     LVRALATGKPAVLDADALNAIAEGEVPAELPRAQCVVTPHPGEAARLLGVTAAGIEAD
                     RFVALQGLRALFPGAVVLKGAGTLVASGAAGGRLGVCPYGNPGMASGGMGDVLSGVLG
                     ALLAQGLAPGDAARLGVCLHARAADLAAAADGERGLLATDLVPWLRRLLNRLAPAQAR
                     A"
     gene            6949..7434
                     /gene="tsaE"
                     /locus_tag="PLW13_11940"
     CDS             6949..7434
                     /gene="tsaE"
                     /locus_tag="PLW13_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQY71134.1"
                     /translation="MNADQSCELRDEDATVGFGARLAAALASGAVVYLHGDLGAGKTT
                     LARGLLRALGHTGSVKSPTYTLVEPYAIAGRTVFHFDLYRLADPEELEFLGVRDYLQP
                     GAILLIEWPERGEGHLPAPDLEIRLEPAGAGRRASWRACNSRGAAIVASLGAGDGRAQ
                     S"
     gene            7512..>8156
                     /locus_tag="PLW13_11945"
     CDS             7512..>8156
                     /locus_tag="PLW13_11945"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HQY71135.1"
                     /translation="MRIGLALVFALGSLQAGAGVAVEGLRLWRGTDHTRLVFDVNGPV
                     RHSLTEMHDPERLVIDVENGELAAALHAVDISSTQVERIRSGKYGPDRLRIVLDLHGK
                     VKQKSFVLGPSSGQGNRLVVDLFGPVPRQAPAPVVPTTPAPPVARAPAASPVPPQPQA
                     QAQVQSPAQPEPQPVARSEEPAPVVRAPLPKQPLRGRSILVAIDAGHGGEDPGAI"
BASE COUNT         1142 a         2796 c         3018 g         1200 t
ORIGIN      
        1 gaggtcgaca cgccgcagct ctgccccgtc accgcgaccg acccgctgct cgagagcata
       61 cccgccctgc ccttcggcga tgactcgccc tggttcctgc agaccagtcc ggagttcgcg
      121 atgaagcgcc tgctggccgc cggcagcgga ccgatctacc agatggcgca cgccttccgc
      181 cgccgcgagt cggggccgcg ccacaacccc gaattcacga tgctcgagtg gtaccgtccc
      241 ggcttcgata ccgcggcgtt gctcgccgag gtcgcgcaac tggtcggcga ggtcatcggc
      301 gcgcgcccca ctcgccgcga cgcctggggc gcgctgttcc tgcgccacgc cggactcgat
      361 cccttcgccg ccagcgacgc ggcgctcgct gagcgcgccg cggccatcgc gggcgaggcc
      421 gcgcgcggct ggccccgcga cgagctgctc gacctgctgt tcagcgagct ggtggagccg
      481 cggctcgggc acgattgcct gcagttcgtg gacgccttcc cggcctcgcg cgcctcgctc
      541 gcgcgcacca cgctcgatgc cgccggcaac ccggtggccg agcgcttcga gctcttcatc
      601 gacggctacg agatcgccaa cggctacaac gaactgtgcg atgccgacga gttgcgccgg
      661 cgcatggatg ccgacaaccg cgcgcgcgcc gcgcgcggcc tgccgccgag cccgcccgac
      721 gagcgcctgc tcgcggcgat ggccagcggc ctgccggaat gcgcgggcgt ggcgctgggc
      781 gtcgaccgcc tgatcatgat cgcgctgggg gcgcagcaca tcggcgaggt gatggcgttt
      841 tcggccgagc gcgcgtgagt ggcgcggtcg tgcgccttcg cggagatggt ccgatggcat
      901 gagcggaccc ggacacagat ccgatccggc gcgccgaggg gcgggcccgt ttcgcgcgcg
      961 gccgccgctt ggcacgtccc tgtgcagcgg ctacgcgtgt tgccgaaatc gctcccggcg
     1021 atttcggcat ccacatgtcc gctcgcgaaa cggacccacc cctcggcgcg cctgccgttg
     1081 gcgctgcctg gtcggtgtgc agtttcgacg aaaacaggtg accgctgcgg ctgaaacgcg
     1141 ttggatcgcg tagattcgac agattgcaca aggactatga gcggtgtcgg agcaccaagg
     1201 cagccggtgc catgctggct gtcttgcgag gaccgtctgt cgcctggatg gcgacagtcg
     1261 agcctacagg gatgtattca cggcgtgtcc gagcaggaga gccggcatgg caccggcggc
     1321 gatggaccgc aggtctatcg caagcgtggc ttacgtccaa cgcctggcgt cgggcgtcag
     1381 ttcgacagcg atccgatccg ctgccccatg cgcagcggcg agcccgcggc gagcgcctcg
     1441 tcccagctcg cgacgccggg cggcagcagc aggatgaccg tggagccgag ctggaagcga
     1501 cccatctcgg cgcccttttc cagcgtgcgt gccgcggcat cgagttcgta atccacgttg
     1561 agcacctgcc ggccggccgg cgtcaccggg ccagtccaca cggtctcgat gcccgcgacc
     1621 accatcgcgc ccaccagcac catcgccagc ggaccgtgct cggtgtcgaa caggcagacc
     1681 aggcgctcgt tgcgcgcgaa cagccccggc accgactcgg ccgtgacctg gttcaccgag
     1741 aacagctttc ccggcacgta gcgcaacgtg cgcaggcgag cgccgagcgg catgtgcacg
     1801 cggtggtagt cgcgcggcga gagatagatc gtggcgaagg agccgttggc gaacaggcgc
     1861 gcgtcggcgg ggtcggccag cagctcggcg gcgctgaacc agtgaccctt ggcctggaac
     1921 atgcggccgt cctcgatcgc gccgagctgg ctcaccgcgc cgtcggcggg cgaggtgatc
     1981 gcctgcggtg ccctatcgac ggggcgcatg ccctcgcgca gcgcgcgcgt gaagaaggcg
     2041 ttgaagtgcg ggtaggcgcc cgggtccggc tcggccgcct cgctcatgtc gaccgcgaac
     2101 tgcgcgatga agcggcggat cagccaatcc ttcagcggct ggaagcccga gctcgcgagc
     2161 atgccggtca ggcgcgacag caggtgttgc ggcagcaggt gctgcatcgc gatgaagagg
     2221 cgctcgcgca ggctgcgtgc gtcggcattc atgatggttc tccggtgatt tgcaggaagc
     2281 tcgcgtatcg cgccgcgctg acggtgcccg ccgcgacggc ctcgcgcagc gcgcagccgg
     2341 gctcgttgcg gtgtacgcag tcgcggaagc ggcagcgccc gaggtgcgga cggaactcga
     2401 cgaagccgtg cgcggtgcgc gccgcatcga ggtgcgcgag gctgaactcg cggatgccgg
     2461 gcgagtcgat caggtcgccg cccgcgggca ggtggtagag ccgcgctgcc gtggtggtgt
     2521 ggcgcccctt ggtcaccgcg tgcgacagct caccgatgcg gatgtcctgc tcgtgcggca
     2581 gcagcgccga gatcagcgag gatttgccca cgccggactg gccgacgaag gcggtggtgt
     2641 gcccggccag cgcggcctgg agctcgtcca ggccgccggc atgcgccgag gcgtgcagca
     2701 ccgcgtagcc gatggcgcgg tagacgccga gcagcggctc gagcgcggcc gggtcagtca
     2761 gcagatcggt cttgttcagc aggatcagcg cctgcagccc gacatcctcg gctgccgcga
     2821 ggtagcggtc gagcaggttg gcatgcggtg gcggttccgg cgcgatcacg atcacgatgc
     2881 gatcgaggtt cgcggcggcg gggcgcagcg tgccgtgcat gtccgggcgg ctcagcacgg
     2941 tcgcgcgctc gtggcgcgcg gtgacgatgc cgccttcgcc gtcgcgatgc cacaccacgc
     3001 gatcgccggc gaccagcgag tcaatgttgg agcgcatgtg gcagcggcac acggttcccg
     3061 ctgcacgcgg atcctccacg tcgacccgct tgccgaagcg cgatatcacc acgccgtcga
     3121 gcgcgggaga gtcgggatcg ggttcgctgg cggcgtgctc ggtgcgccgg cgctgcaact
     3181 tctcgacccg gcgcgcctgg ttctggctca gcttgcgtcc ggccatgggc tgttcagcgt
     3241 gtcagcgcgg cgaacgccgc agcgcgcggg ggcgagggca ggcgaagcgg gaaaaggctt
     3301 gttgcataat gcgggcgtaa cggggtctta tcatcgaacg tttggcgcgg gagtctagca
     3361 tgatgctcga ggacggattg gtctgggtcg acctggaaat gaccgggctc gacccggatc
     3421 gcgacgtggt catcgagatc gcgaccatcg tgaccgatgc gcacctgaac gtgatcgccg
     3481 aaggcccggt attcgcgatc caccagggcg acgcggtgct ggcgacgatg gacgagtgga
     3541 acacgcgcca gcacggccag tcggggctga cccaacgcgt gcgcgagagc cgcctcgacg
     3601 aggaggccgt ccaggacctg acgctggagt tcctgcgccg tcacgtgccc cccggcaagt
     3661 cgccgatgtg cggcaacagc atctgccagg accgacgctt catggcgcgc cgcatgcccc
     3721 gcctcgaggc cttcttccat taccgcaacc tcgacgtcag caccgtgaag gagctcgcgc
     3781 gccgctggcg tcccgatgtg cttgccaccg tcaccaagca gggcgcgcat ctcgcgctcg
     3841 acgacatccg cgactcgatc gcggagttgc gtcactaccg cgagcacttt tttcgcatga
     3901 cgccctgagg tcgtagcgca tcgctggctg tgctggcccc ccggtcgttc gggggacgct
     3961 ttgaatacat ccctgtacgc tcgagcgccg catccttgcg gcgcacgccc cctcacgcct
     4021 gtgcgtccag cacagccgct cggcaaagtt cgtggtacca caaacgcagg tcgcggcgcg
     4081 ggccaggcgt gatgcgggac cgtctgcggc atggatgccg cagtcgagcg tacatggacg
     4141 tattcacagc gtgtcccgca acatgcctgg cccgcgccgg gaccgggcta cggagttgtg
     4201 ccttgagaaa cgggcctcgt cattgagtgg cgagttgctc cagagcccac gtgacatgct
     4261 cccggaccaa ttccgaggga tgttctgacc gcgccagcag cgccgcgcgt gccgcatccg
     4321 tggcgggtcc gttgccgagc gccacggcga ggttgcgcag ccagcgttcg tggccgaggc
     4381 ggtagagcgg agtgccggcg gcgcgctcgg cgaattgctc cgcgctccac gcgaacagtt
     4441 cggtgagcgt tgcggcatcg aggccgtggc gcggcgcgaa gtcggcctcg cagcttgggc
     4501 gcgcgaactt gttccacggg cacaccagct gacagtcgtc gcagccgaag acgcggttgc
     4561 cgatcagcgg gcgaagatcc tgcgggatcg cgccctgcaa ctcgatcgtg agatatgaaa
     4621 tgcagcgccg cgcgtcgagc acgaagggcg acacgaaggc agcggtggga cagacgtcga
     4681 gacaggcggt gcaactgccg caatgcatgc cgctgaattc ggcgtccagc ggcagcggga
     4741 tgtcggtata gatctcgccg aggaagaacc acgagccggc gcgcgggtcg atcagcaggg
     4801 tgttcttgcc gatccagccg aggccggcct tctgcgccac gccgcgctcc agcaccggcg
     4861 cgctgtcgac gaacacgcgc tgcgcgctgc cggggcgcgc ggcgaggatg cgatccgcca
     4921 gccgcgccag ccgcgcgcgc atcagcttgt ggtagtcgcg tcccagcgcg tagcgcgaga
     4981 cataggcgcg ctcggcgtcg ttcaggatgc cggaaggctc ggcgctgtcg gcacggtggt
     5041 agtccatgcg caccgagatc acgcgcagcg tccccggcag caactcctcg gggcgccagc
     5101 gcctggcccc gtggcgcgtc atgtagtcga gctcgccgtg caggccctgc gctacccact
     5161 cccgcaggcg ctcgtcgtgg ggagacaggt cggtgccgca gatgccgagc gaggggaagc
     5221 ccagttcgcg cgcccaggcg cggatatcga cggcgagcgc cgccagcgag cccgtggcgt
     5281 cttcccgggc tgaatcggtc gtgatcatcg gcagcgccct gcagcgtgga gcgatcgggt
     5341 caggggagtg ccgcccgacc cgcggccagc ataacggagg catcgcgtac aatgaaaacc
     5401 ggtccaacgg agagccctcg cgatgcctga tgccgagctg cagcccgcgc tctacaccgc
     5461 cgcggagacg cgcgcgctcg atcgcgccgc gatcgacggt gcgggcattc cgggcatcgt
     5521 gctgatgagc cgcgccgggc gcgcggtgtt cgagctcacg tgcgcgcgtt accccggcgt
     5581 gctgccggtc catgtcgtct gcggggccgg caacaacggc ggcgacggct tcatcgtggc
     5641 gcggctcgcg cgcgaacgcg gcctggcggt gacggtgtcg ctgctcggcg agccggcgcg
     5701 catcgcgggt gatgcgcggc tcgcgctgga ggccgcgctc gcggcgggcg tccaggtgcg
     5761 tccgttcacg ccgcagctgc gtttcgatgc gggcgtcatc gtcgatgcgc tgctcggcac
     5821 cgggctcggc gggccggtgc gcgaggcgca ggcgcaagcc atcgcggcga tgaacgccag
     5881 cgggctgccc atcgtggcgg tcgacattcc ctcggggctg tgcagcgata ccggttgcgt
     5941 gcgcggcgtg gcggtgcgcg ccgcgcacgc cgtgacattc atcgggcgca agcgcgggct
     6001 gtacacggcg gcagcgcgcg atcattgcgg cgagctgcat ttcgatgcgc tcggcgtacc
     6061 ggcgcaaacc tatgacgcgg tgccggccgc ggccgagctg ctgtcgctcg aggacctgct
     6121 ggcggcgctg gcgccgcgcg cgcgcgctgc gcacaagggg agtttcgggc acgtgctggt
     6181 gatcggcggc gggcgcggca tggcgggcgc cgccgcgatg gcggcggagt cggccgcgcg
     6241 ttgcggcgcg gggctggtgt cgctcgccac ctggccgggc aacgtggccg cgacgctggc
     6301 gcgccgtccc gagatcatgg cgcacggcgt ggagcacggc catgagctgg agccgctgct
     6361 cgcgcgcgcc ggcgtgctgg tgatcggccc ggggctgggt cgcgggccct gggcgcagca
     6421 gctgctggtg cgcgcgctcg ccaccggcaa gcccgcggtg ctggacgccg atgcgctgaa
     6481 cgccattgcc gagggcgagg tgcccgcgga gctgccgcgc gcgcagtgcg tcgtgacgcc
     6541 ccatcccggc gaggcggcgc ggctgctcgg cgtgacggcc gccgggatcg aggccgaccg
     6601 tttcgtggcg ctgcagggcc tgcgtgcgct gtttccaggc gcggtggtgc tcaagggcgc
     6661 cggcaccctg gtggcgagcg gcgcggccgg cgggcgcctc ggcgtctgcc cctacggcaa
     6721 tcccggcatg gcgagcggtg gtatgggcga cgtgctgagc ggagtgctcg gcgcgctgct
     6781 ggcgcagggg ctcgcacccg gcgatgccgc gcgtctgggc gtctgcctgc acgcgcgcgc
     6841 cgcggatctc gcggcggccg ccgacggcga acgcggcctg ctcgccaccg acctggtgcc
     6901 gtggctgcgg cgcctgttga accgcctcgc gccggcgcag gcccgggcat gaacgccgac
     6961 caatcctgcg aactgcgcga cgaggacgcg acggtcggct tcggggcgcg gctggcggcc
     7021 gcgctcgcca gcggcgcggt ggtctacctg catggcgacc tgggcgcggg caagaccacg
     7081 ctcgcgcgcg ggctgctgcg cgcgctcggc cacaccggtt cggtgaagag tcccacctac
     7141 accctggtcg agccctatgc gatcgcgggg cgcaccgttt tccatttcga tctctaccgg
     7201 ctcgcggatc ccgaggagct cgaattcctc ggcgtgcgcg attacctgca gcccggggcg
     7261 atcctgctga tcgagtggcc ggagcgcggc gaggggcacc tgccggctcc ggatctcgag
     7321 atccggctgg aacccgccgg cgcggggcgc cgtgcgagct ggcgtgcatg caacagccgc
     7381 ggtgccgcaa tagttgcctc gctcggcgcc ggcgatgggc gtgcgcaatc ctgagcggtt
     7441 gctatatttt ggatcggcat attccgcgga tccggggacg catgacttta cggccaagaa
     7501 actcagtgtg cgtgcgcatc ggcctcgccc tggtgttcgc cctgggcagc ctgcaggcgg
     7561 gcgccggtgt tgcggtcgag ggcctgcgcc tgtggcgggg cacggatcac acgcgcctgg
     7621 tgttcgacgt gaacggcccg gtgcgccaca gcctcacgga aatgcacgac cccgagcggc
     7681 tggtcatcga cgtcgagaac ggcgagctcg ccgcggcgct gcacgcggtg gacatcagca
     7741 gcacacaggt ggagcgcatc cgtagcggca agtatggccc cgaccggttg cgcatcgtgc
     7801 tcgacctgca cggcaaggtg aaacagaaga gcttcgtgct gggcccgagc agcggccagg
     7861 gcaaccgcct cgtggtggac ctgttcggcc cggtgccgcg ccaggcgccg gcgcccgtgg
     7921 tgcccacgac tcccgcgccg ccggtggcgc gggcgcccgc cgcgagtccc gtgccgccac
     7981 agccccaggc gcaggctcag gttcaatcgc cggcccagcc cgagccgcag ccggtggcgc
     8041 gtagcgagga gcccgccccg gttgtgcgcg cgccgttgcc gaaacagccg ctacggggtc
     8101 gcagcatcct ggttgcgatc gacgccgggc acggcggtga ggatccgggt gccatc
//
LOCUS       DAORUQ010000145         8132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9833_length_8132_cov_6.316702, whole genome shotgun sequence.
ACCESSION   DAORUQ010000145 DAORUQ010000000
VERSION     DAORUQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_8132_cov_6.316702"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLW13_11950"
     CDS             <1..455
                     /locus_tag="PLW13_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY71136.1"
                     /translation="RGARFDDYISAMKKVWSGELVEHHSPFLDWSGFKSYPLPVQKPF
                     PVIIGGSKGKAFERIARYGTGWYAPCESTDDLAPMLDALKHACSALGRDFAQIEITAM
                     WRPELGIERVHAMRELGVHRLVARMIPDPRHDLLAQIARVGEDVIAKL"
     gene            591..>2773
                     /locus_tag="PLW13_11955"
     CDS             591..>2773
                     /locus_tag="PLW13_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71137.1"
                     /translation="MRRCVRAIRPSFLIAPLAFAIQCVPVHADAGLELEEIVVTAQRR
                     AESILEVPISMAVETGVTLEKKGIATLEGLSLQTPSLITQDGGRVSSVAMRGLGSPGL
                     DTVESSVGIYIDEVYFGRSRLSRNPMFDMDRIEVLRGPQGTLYGRNTIAGAISMYTAR
                     PSDEFSARVLGEYGNIDSHKVEGYVSGPITDTLSGRIAGINSERGTYLDNSIGPDGGG
                     QDSEGYRLSVGWTPAETVYVFAKYEHMTHENIGMYDQLVGDPFGVWADYPGIDLKQDD
                     KQQVNGPGLQGLNEPGGRYDADIAALHVNWDLPGDYALKSVTGWSEYDARSRDWISAS
                     PDNALTINGLTERNEYYSQELRIESPTDRRFHFVAGGFVDSFDVHTLPRAEDFAALNL
                     GTQILPPLVDGLATNPQLGFLGDLQDNVAQGYANGTAEYFRLITPSGSPDGRISNLDQ
                     EIRTWSLFFEGEYEFTEQWHLVLGVRYTDEENEFALSKGTWYTNGLGLPWGAYPSANE
                     IAASALAADPSLAPWAALLPVIYGATSSAEARPGYTVSQLPTYIAAPGGTPRAEDRLE
                     DDQWIPTAKLQYFLDDDTMLYFTVASGFKAGGVNSGNINVYTREGDTFGAEEALSFEL
                     GGKFTLLDGAAQLNVALFRTDFDDLQTATITPQGAVTVVNAAAAVTQGVDLDATWRLS
                     ESVTVGGAYSWLDAYYDDSEELNCGSYMTNVRAAAGEDFSTTPCT"
     assembly_gap    2774..2873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2874..3295
                     /locus_tag="PLW13_11960"
     CDS             <2874..3295
                     /locus_tag="PLW13_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71138.1"
                     /translation="TNVRAAAGEDFSTTPCTYRLDDMPNGDDALQRAPEHTVTLWGEY
                     FAPVADGWDLQLYGSVNYRDESSTTLDNYFYADDLTMVGARVALHDTRRKWSVALFGN
                     NLLDEDGLVLHQENSAGAIKGMITTPRTYGLQLVKEW"
     gene            complement(3316..3948)
                     /locus_tag="PLW13_11965"
     CDS             complement(3316..3948)
                     /locus_tag="PLW13_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71139.1"
                     /translation="MRVAGETSAHAHHHPRRHTARLLGAGLAGGVVVLAATLLFAGGD
                     DSWALAARHTARFSALWFMVAFVAGPLARITRASWARVLVRERRGIGLGFCAAHTVHL
                     AAILLWLGHGGKADAATLAGGGLAYLFLLAMAATSNDAAVRRLGARRWLALHTVGQFY
                     LWLIFAQTYLGRVLAPDARPVHLALAVGMAAAMGLRLFTVLTRFDRRRSA"
     gene            4152..5117
                     /locus_tag="PLW13_11970"
     CDS             4152..5117
                     /locus_tag="PLW13_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY71140.1"
                     /translation="MKTIPLPRTGLTVSQLCLGTNQFGTAQDDARAHEMLDTFRALGG
                     NFIDTAHGYADWIPTAPRAASEHCIGRWLARQRRDSVVIATKGCEFDYRAGFTPRVTP
                     EHLGADLEGSLAALGTDYIDLYWLHRDDPSHPVEAILDALIAHQRAGRIRHFGCSNWS
                     VARIEQAQRYAASIGHEGFVACQPMWGLAEPDRAAMQQFAPGGYYEDGYRALHAGGLT
                     MIPYSGQSRGIFTRLAAGQEESIAADVRALYLSDTNRRKLGVVRELALQHNATVNAVV
                     LAYLTSQPLPTVPIIGASSLAQLRESVGSCALVLSVEELDTLRNA"
     gene            5140..6786
                     /locus_tag="PLW13_11975"
     CDS             5140..6786
                     /locus_tag="PLW13_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQY71141.1"
                     /translation="MSRADAIADVCIVGSGAGGGVMAAELARAGIGVVLLERGPERTS
                     EDFLHRQDELTNVLRQDFFAPGYRETLRASEREQAVPGRYSMLAQTLGGSTVHWGAWS
                     WRFRADEMRMLSRDGPIAGASLADWPIDYAELEPFYAQAEQALGIAGRAGSNPFETPR
                     TGAYPNPPHVYRPSSRLLEAGAKKLGLHPFPLPMAINSRVFDGRAKCMNGGQCAGFGC
                     PVNAKASPLAIHIPAARATGRAEVITGARALEIGVDAEGRARGVHYRAADGATREVRA
                     RHVIVCAGSIGSATLLLGSRSARFPNGLANGSDQVGRNLMCHIFSLVNFEMREPSLGQ
                     LGPPGNVGVDDFHPSDARRGFVGGAVLGEAIDPLPIVSALKSKDYLRGQRAWGEPLSR
                     YLERYPHYCGMIAIGEDLPVASNRVDLDPDHRDAEGVALPRITHTNHPNDVALARYME
                     ARMSEIATAGGALKTFATDYVGARTATGHIMGTCRMGSDPRNSVLDRWCRSHEVANLW
                     VVDGSFFPTSGGYNPTLTIFANAYRVAAHFIAEARRLNLG"
     gene            6791..7345
                     /locus_tag="PLW13_11980"
     CDS             6791..7345
                     /locus_tag="PLW13_11980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQY71142.1"
                     /translation="MERREFLQVFGAALGAALLARPLAAAAAASTQQALVLNASECAL
                     LEAICARILPPVGGVSTREAGCVNFIDKLLAHEERALAPAYRAGLAALDALARGFAAL
                     EAAQQVHLLEQLEDGTLQPWPEAAGTQATLFGLLRMHTLLGFLAAPSFGGNRDGAGWK
                     AVGHVGHLHGAGGVSDEQVAGKSV"
     gene            7495..>8132
                     /locus_tag="PLW13_11985"
     CDS             7495..>8132
                     /locus_tag="PLW13_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71143.1"
                     /translation="MSNAIRIDDLREPRLSPAQQAVIDATAGLQVELSVEEVLGAARE
                     RTGLADFGPEDFLERLGLLVDEWSSDAAMRPLQRLNLRNYLVRYAANRLLLQDLWTRH
                     PEILETQIRQPIIVAGLPRSGTTHLVNLLAADRRFHSLPLWESYEPVALPGEPLLPDG
                     TDPRYGRCAEAWAGMQATLPLLAAMHPMNPDHIHEELELMGPDFASYNFEWL"
BASE COUNT         1308 a         2888 c         2691 g         1145 t
ORIGIN      
        1 accgcggcgc gcgctttgac gattacattt cggcaatgaa aaaggtctgg tcgggcgagc
       61 tggtcgaaca ccacagcccg ttcctggact ggagcggctt caagagttac ccgctgccgg
      121 tgcagaagcc cttccccgtg atcatcggcg gcagcaaggg caaggccttc gagcggatcg
      181 cccggtacgg caccggctgg tacgcgccgt gcgaatccac cgacgatctc gcgcccatgc
      241 tcgacgcgct gaagcacgcc tgcagcgcgc tcgggcgcga cttcgcgcag atcgagatca
      301 ccgcgatgtg gcgacccgag ctcggcatcg aacgcgtgca cgcgatgcgc gagctcggcg
      361 tgcaccgact ggtggcgcgc atgatccccg acccgcgcca cgatctgctc gcacagatcg
      421 cgcgcgtggg cgaggacgtc atcgccaagc tctgaaacac ccgctcgcga ttaccatcgc
      481 actcgattgc attgacgcat cttactgggt actctccgga gcgttcctgg cgctccgcac
      541 tgccggacgc cggctcccga tgtccataac aattaccgag gagagggttc atgcgtcgtt
      601 gcgtccgtgc cattcgtccg tccttcctga tcgctccgct cgccttcgcc atccagtgcg
      661 ttcccgtgca tgccgatgcc ggcctcgagc tcgaggaaat cgtggtcacc gcgcagcggc
      721 gcgccgagag catcctcgaa gtgccgatct cgatggcggt cgagaccggc gtgacgctgg
      781 aaaagaaagg catcgccacg ctcgagggac tgtcgctgca gacgcccagc ctgatcacgc
      841 aggacggcgg gcgggtgtcc tcggtggcga tgcgcggcct cggcagcccg gggctcgaca
      901 ccgtcgagag ctcggtcggg atctacatcg acgaggtcta cttcgggcgc agccgcctgt
      961 cacgcaaccc gatgttcgac atggatcgca tcgaggtgct gcgcggcccg cagggcacgc
     1021 tgtacgggcg caacaccatt gccggcgcga tcagcatgta taccgcgcgg ccttcggacg
     1081 agttcagcgc gcgcgtgctc ggcgaatacg gcaatatcga ttcgcacaag gtcgaaggct
     1141 atgtctcggg gccgatcacc gacacgctgt cgggccgcat cgcggggatc aacagcgagc
     1201 gtggcaccta cctcgacaac agcatcggcc ccgatggcgg cggacaggac agcgaggggt
     1261 atcgcctcag cgtcggctgg acgcctgccg agacggtcta cgtgttcgcc aagtacgagc
     1321 acatgacgca cgagaacatc ggcatgtacg accagctggt cggtgatccc ttcggcgtgt
     1381 gggcggacta tccgggcatc gacctgaagc aggacgacaa gcagcaggtg aacggtccgg
     1441 ggctgcaggg gctcaacgag cccggcgggc gctacgacgc cgacatcgcg gcgctccacg
     1501 tgaactggga tctcccgggt gactacgccc tgaaatcggt gacgggctgg agcgagtacg
     1561 acgcgcgctc gcgcgactgg atctccgcct cgcccgacaa cgcgctcacg atcaacggcc
     1621 tgaccgagcg caacgagtac tacagccagg agctgcgcat cgagtcgccc accgaccgtc
     1681 gtttccactt cgtggccggc ggcttcgtcg acagcttcga cgtccacacg ctgccgcgcg
     1741 ccgaggactt cgccgcgctc aacctcggca cgcaaatcct gcctcccctg gtggacggcc
     1801 tcgcgaccaa cccccagctc ggcttcctcg gcgacctgca ggacaacgtc gcccagggct
     1861 acgccaacgg caccgccgag tacttccgcc tgatcacgcc ttcgggatcg cccgacgggc
     1921 gcatcagcaa cctcgaccag gagatcagga cctggtcgct gttcttcgag ggcgagtacg
     1981 agttcacgga gcagtggcat ctggtgctcg gcgtgcgcta caccgacgag gaaaacgagt
     2041 tcgcgctgtc caagggcacc tggtacacca acggactcgg cctgccgtgg ggcgcctacc
     2101 cctccgccaa cgagatcgcg gcgagcgcgc tggcggcgga tccctcgctg gcgccatggg
     2161 cggcgctgct gccggtgatc tacggcgcca ccagcagcgc ggaggcccgt cccggctata
     2221 ccgtcagcca gttgcccact tacatcgccg cgcccggcgg gacgccgagg gccgaggacc
     2281 ggctggaaga cgaccagtgg atccccacgg ccaagctgca gtacttcctg gacgacgaca
     2341 ccatgctgta cttcacggtc gcctcgggct tcaaggccgg cggcgtcaac agcggcaaca
     2401 tcaacgtcta cacccgcgaa ggcgacacct tcggtgccga ggaggcgctg tccttcgaac
     2461 tgggcggcaa gttcacgctg ctcgatggcg cggcccagct gaacgtcgcg ctgttccgca
     2521 ccgatttcga cgacctgcag accgccacga tcacgccgca gggcgcggtc accgtggtga
     2581 acgcggcggc cgcagtcacg cagggcgtgg acctcgacgc cacctggcgc ctgagcgaga
     2641 gcgtcacggt gggcggcgcc tactcgtggc tcgacgccta ttacgacgac tccgaggaac
     2701 tgaactgcgg cagctacatg acgaacgtgc gcgcggcggc cggcgaggac ttcagcacca
     2761 cgccctgcac ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgacgaa
     2881 cgtgcgcgcg gcggccggcg aggacttcag caccacgccc tgcacctacc gcctcgacga
     2941 catgcccaat ggcgacgacg cgctgcaacg cgcgccggag cacacggtca cgctgtgggg
     3001 cgagtacttc gcgccggtcg ccgatggctg ggatctgcag ctctacggct ccgtgaacta
     3061 ccgtgacgag tcctcgacca cgctcgacaa ctacttctat gccgacgacc tgacgatggt
     3121 gggcgcacgc gtggcgctcc acgacacgcg acgcaagtgg tcggtggcgc tgttcggtaa
     3181 caacctgctc gacgaggacg gcctggtgct gcaccaggag aactcggcgg gcgcgatcaa
     3241 ggggatgatc accacgccgc gcacctacgg gctgcaactc gtcaaggagt ggtagcgcgc
     3301 accgtgggtc cgcattcatg cggatcgcct gcggtcgaat cgtgtcagca ccgtgaacag
     3361 gcgcaagccc atggccgcgg ccatcccgac cgccagcgcc aggtgaaccg gcctggcgtc
     3421 gggcgcgagc acgcggccga gataggtctg cgcgaagatc aaccacaggt agaactgccc
     3481 gaccgtgtgc agcgccagcc agcgccgcgc gccgagccgg cgcacggcgg cgtcgttcga
     3541 cgtggccgcc atcgcgagca ggaacaggta cgcaaggcca ccacccgcca gggtcgccgc
     3601 atccgctttg ccgccatgac cgagccacag caggatcgct gccaggtgca ccgtgtgcgc
     3661 agcgcagaat cccaggccga tgccgcggcg ctcacgcacc agcacgcgcg cccacgacgc
     3721 gcgcgtgatg cgcgccagcg gaccggcgac aaaggccacc atgaaccaca gcgcggagaa
     3781 gcgtgccgta tgccgcgccg ccagcgccca actgtcgtcg ccgccggcga acagcagcgt
     3841 tgccgccagc accacgacgc cgcccgcgag acccgcgccc agcaggcgcg ccgtgtgccg
     3901 ccgcggatgg tgatgcgcat gcgcgctggt ttcgcccgcc accctcatcg ccgcctccct
     3961 tgccgcgcct tcgtcacgca cgcactctac cccgccgcgc gtgtcgtttc atgcctgccc
     4021 ggtcggcggc atggcgccgg cgggtggctg agcccctgcg tcccgcggtt gagcgccgcc
     4081 gtcctgtaca gaccgccgca atcggctagg atcggtaccc cggttcaacc gccaagcaca
     4141 gagagaacgc catgaagacg attccgctgc cccgcaccgg cctcacggtc agccagctgt
     4201 gcctcggcac gaaccagttc ggcaccgcgc aggacgacgc gcgcgcccac gagatgctcg
     4261 acaccttccg cgcgctcggc ggcaatttca tcgataccgc gcacggctac gcggactgga
     4321 tcccgaccgc gccgcgcgcg gcctccgagc actgcatcgg ccgctggctg gcgcgccagc
     4381 ggcgcgacag cgtggtgatc gccaccaagg gttgcgaatt cgactaccgc gccggcttca
     4441 cgccgcgcgt gacaccggag cacctcggcg ccgatctcga gggcagtctc gccgcgctcg
     4501 gcacggacta catcgacctg tactggcttc accgcgacga tccctcgcac ccggtcgagg
     4561 ccatcctcga cgcgctgatc gcacaccagc gcgccggacg gatccgccat ttcggctgct
     4621 cgaactggag cgtggcgcgc atcgagcagg cgcaacgcta cgcggcctcc atcggccacg
     4681 agggcttcgt cgcctgccag ccgatgtggg gcctggccga gccggatcgc gccgcgatgc
     4741 agcagttcgc cccgggtggc tactacgagg acggctaccg cgcgctgcac gcaggcgggc
     4801 tcacgatgat cccctactcg ggccagagcc gcggcatctt cacccggctc gccgccgggc
     4861 aggaggaatc gatcgctgcg gacgtgaggg cgctgtacct ctcggacacc aaccggcgca
     4921 agctcggcgt ggtgcgcgag ctggccctgc agcacaacgc cacggtgaac gccgtggtgc
     4981 tcgcctacct caccagccag ccgctgccga cggtcccgat catcggcgcg agcagcctgg
     5041 cgcagctgcg cgagagcgtg ggctcctgcg cgctggtgct gtcggtcgag gagctcgaca
     5101 cgctgcgcaa cgcatgaagg cggcggcgga gcgcgcgcga tgagccgcgc cgacgcgatc
     5161 gccgacgtgt gcatcgtcgg ctcgggcgcg ggcggcggcg tgatggccgc cgagctcgcg
     5221 cgcgccggca tcggcgtggt gctgctggaa cgcggcccgg agcgcaccag cgaggacttc
     5281 ctgcaccgcc aggacgaact gacgaacgtg ctgcgccagg acttcttcgc gcccggttat
     5341 cgcgagacgc tgcgcgccag cgagcgcgag caggccgtgc ccggtcgcta cagcatgctg
     5401 gcgcagacgc tgggcggcag cacggtgcac tggggcgcct ggagctggcg tttccgcgcc
     5461 gacgagatgc gcatgctgag ccgcgacggc cccatcgccg gcgccagcct cgccgactgg
     5521 ccgatcgact atgccgagct cgagcccttc tacgcgcagg ccgagcaggc gctcggcatc
     5581 gcggggcgcg ccggctcgaa ccccttcgag acgccgcgca cgggcgccta cccgaacccg
     5641 ccgcacgtct atcggccgtc gtcgcgcctg ctcgaggccg gcgcgaaaaa gctcgggctg
     5701 caccccttcc cgctgccgat ggcgatcaac agccgcgtct tcgacggtcg cgccaaatgc
     5761 atgaacggcg ggcagtgcgc gggcttcggt tgcccggtta atgccaaggc ctcgccgctg
     5821 gcgatccaca tccccgcggc acgcgccacc ggcagggccg aggtcatcac cggggcgcgc
     5881 gcgctcgaga tcggcgtgga cgccgagggg cgggcgcgcg gcgtgcacta tcgcgccgcg
     5941 gacggcgcca cgcgcgaggt gcgcgcgcgc cacgtgatcg tctgcgccgg ttcgatcggc
     6001 agcgccacgc tgctgctcgg ctcgcgctcg gcgcgcttcc cgaacggact ggccaacggc
     6061 agcgaccagg tcgggcgcaa cctgatgtgc cacatctttt cgctggtgaa cttcgagatg
     6121 cgcgagccct cgctggggca gctcggcccg cccggcaacg tcggcgtcga cgacttccat
     6181 ccctcggacg ccaggcgcgg cttcgtcggc ggcgcggtgc tgggcgaggc catcgacccg
     6241 ctgcccatcg tgagcgcgct gaagagcaag gactacctgc gcgggcagcg tgcctggggc
     6301 gaaccgctca gccgctacct cgaacgctac ccgcactatt gcggcatgat cgcgatcggc
     6361 gaggacctgc cggtcgcatc gaaccgcgtc gacctcgacc cggatcatcg cgatgccgag
     6421 ggcgtcgcgc tgcctcgcat cacgcacacc aatcatccga acgatgtcgc gctggcgcgt
     6481 tacatggagg cgcgcatgag cgagatcgcc acggccggcg gcgcgctgaa aaccttcgcc
     6541 accgactatg tcggcgcgcg caccgcgacc ggccacatca tggggacctg ccgcatgggc
     6601 agcgacccgc gcaactcggt gctggaccgc tggtgccgca gccacgaggt ggcgaacctg
     6661 tgggtggtcg acggcagctt cttcccgacc tcgggcggct acaacccgac gctcaccatc
     6721 ttcgccaacg cctaccgcgt ggccgcgcac ttcatcgccg aagcgcgccg gctgaacctg
     6781 ggctgatcgc atggaacgcc gcgaattcct gcaagtcttc ggcgccgccc tgggtgccgc
     6841 gctgcttgcc cgtccgctcg cggctgccgc cgcggcatcc acgcagcagg cactggtgct
     6901 gaacgcatcc gagtgcgcgc tgctcgaggc aatctgcgcg cgcatcctgc caccggtcgg
     6961 cggcgtgtca acgcgcgagg ccggctgcgt gaacttcatc gacaagctgc tcgcgcacga
     7021 ggagcgggcg ctggcaccgg cgtatcgcgc cggactcgcc gcgctcgacg cgctggcgcg
     7081 cgggttcgcg gcgctcgagg ccgcgcagca ggttcacctg ctcgagcaac tggaagacgg
     7141 gacactgcag ccctggcccg aggccgccgg cacgcaggcc acgctgttcg gcctgctgcg
     7201 catgcacacg ctgctcggct tcctcgcggc gccgtccttc ggcggcaacc gtgatggcgc
     7261 cggctggaag gcggtgggtc atgtcgggca cctgcacggg gcgggcggcg tcagcgacga
     7321 gcaggtggcg ggcaaatccg tctagacgag cgcggccgca gtggactgcc ggtcgtgccc
     7381 gggcggcgct gtcaggggtt tcgatttcag ccgaacgcat gagccacggc gatcgtgcct
     7441 tgggctatat tgcgccgtgc gccgaactga acaccgcgaa ggagatattt cgacgtgagc
     7501 aatgcaatcc ggatcgacga cctgcgcgaa ccgcgcctga gccccgcgca gcaggccgtg
     7561 atcgatgcga ccgccgggct gcaggtcgaa ctctcggtcg aggaggtgct gggcgccgcg
     7621 cgcgaacgca ccgggctggc ggacttcgga cccgaggact tcctcgagcg cctggggctg
     7681 ctggtcgatg aatggagctc ggacgccgcc atgcgcccac tgcagcgcct gaacctgcgc
     7741 aactatctcg tgcgctacgc cgcgaaccgg ctgctgttgc aggacctgtg gacgcgccac
     7801 ccagaaatac tggaaacgca gatccgccag ccgatcatcg tcgccggact gccgcgctcg
     7861 ggcacgacgc acctggtgaa cctgctcgcc gccgaccgcc gattccattc cctgccgctg
     7921 tgggaatcct acgaaccggt ggcactgccg ggcgagcccc tgttgcccga cggcacggac
     7981 ccgcgctacg gtcgctgcgc cgaggcctgg gccggcatgc aggccacgtt gccgctgctg
     8041 gcggcaatgc acccgatgaa cccggaccac atccacgagg agctcgagct gatgggcccg
     8101 gacttcgcgt cctacaactt cgagtggctg ag
//
LOCUS       DAORUQ010000146         8108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9876_length_8108_cov_5.671675, whole genome shotgun sequence.
ACCESSION   DAORUQ010000146 DAORUQ010000000
VERSION     DAORUQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9876_length_8108_cov_5.671675"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLW13_11990"
     CDS             <1..847
                     /locus_tag="PLW13_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71144.1"
                     /translation="GEIWCQGFSEPNAGSDLASLKTRAERRGDRYVINGQKIWSTMAQ
                     YADRCLLLARSGRSGPPQAGLTYLLLDMRSKGVSVRPIAQITGDDEFAEIFFDDVEVP
                     VEDLVGAEGEGWAVAQATLASERGLTLVELSQRMRYALPLLGAAMREAGRQGDAALRR
                     DLGRVMAKVDGACALADWYLLRRISGEEAPGDASMVKLNYARTLREFTSLGLRIGGLQ
                     SQYHRGFMRGATQETGNWMVDFMNSYNWSIAGGSDEIQRNIIAERLLRMPREPKTWQL
                     PEERA"
     gene            844..1896
                     /locus_tag="PLW13_11995"
     CDS             844..1896
                     /locus_tag="PLW13_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71145.1"
                     /translation="MNADLLELQQSVRQVLAGRAVAADEQDTWPLVVELGWLLAGVPE
                     ELGGLGQGLAGARVLQIELGRRLAAVPFLPAALALDAVCQSALADRAQWVERLTTGEY
                     VAAPLAESALDLEPAGGGTARLSGLASGVQSADRASHALVWTRGGDCVALAPLERAGV
                     EIVARPTWDRTHRLFDLRFAGLELDETLVLARGAAAQALVRRLGALRDLGLAADAVGG
                     ANALLELTVEHLQVRRQFGRPLALFQALKHRCADLKTLIAAAEALLLDSLGRLGDSVA
                     DAEAELKAETAKYLASSTYAKVAEEALQLHGGIGMAAEHQCHLFLKRALLNEQLGRQD
                     ERYALDIADSLLARLD"
     gene            1944..2927
                     /locus_tag="PLW13_12000"
     CDS             1944..2927
                     /locus_tag="PLW13_12000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HQY71146.1"
                     /translation="MFSAVVINKGESGQSVELTQVDESQLPEGDVTIDVEYSTLNYKD
                     GLAITGSSPVVRKFPMVPGIDLAGVVRESSHADWKAGDRVILNGWGVGETHWGGLAQK
                     ARLKGEWLVPLPSAFSSRQAMAIGTAGYTASLCVDALLAHGVRPEQGEILVTGATGGV
                     GSVAVALLVKAGFSIAAATGKAAEADYLKQLGASTIIDRAELAAPGKPLQKERWAGVV
                     DCVGSHTLVNACAQTRYGGAVAACGLAQGADFPATVMPFILRGVSLLGVDSVMAPKAR
                     RLVAYERLARDLDGAALDVIATEIGLADAIATAADLLQGKVRGRVVVNVNK"
     gene            complement(2959..3627)
                     /locus_tag="PLW13_12005"
     CDS             complement(2959..3627)
                     /locus_tag="PLW13_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71147.1"
                     /translation="MAGDEKRAAKAAQSSTASGKRSTRSRPALREALLSLLEERPFEQ
                     VTVRELTARAGVGYATFFRHYPDKEALLHDLAAHEISKLLAMAQPILYTVDSRASAEA
                     LCSYVWEYRKLWSALLTGGAAAMLKDEFVRQARQIAAERSSRHAWLPGDLSVVFSVAS
                     TVEILAWWLRQSKPPSVQRMAEILDQLVIIPSMPPVKNAGAGAPPERGSTRRRPGGRR
                     RSTS"
     gene            3776..6277
                     /locus_tag="PLW13_12010"
     CDS             3776..6277
                     /locus_tag="PLW13_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71148.1"
                     /translation="MNRVAVRKILRGSSLAVLGAVSPAYAQGEAGAGELENIIVTARR
                     VEERVQDVPISITVFNQQQLTKQNVVNSADLAAFTPSLSANSNFGNENSSFAIRGFVQ
                     DLGTLPSVGVYFADVVAPRGSSNGSFPVGDGAGPGSFFDLQNVQVLKGPQGTLFGRNT
                     TGGAVLLVPQRPTHELGGYVQASAGNLDMQRLQGVVNAPLGDKAAFRLGVDKQTRDGY
                     VENTSGIGPDDFGDVDYTAYRASLTVELTPDLENYAIFSRSDSRTNGVFGKLVAADAA
                     VGLGFFAAQQLADQEADGDGFYDARQDLANPLSDLQQWQLINTTTWQVHDDLTFKNII
                     SYAELTQDLRSAMFGTAFVTPALGALLPSYRVGFASIQPIPGGHTGDQSTFTEEMRLD
                     GAGFDNALTWQTGAYVERSEPLSAVGSQSPVAISCTDVAALECTDVLGFLGSLATGTY
                     APAGAINYTSGKTEFNNVGLYAQGTYQFSGNLALTAGLRYTWDRQESTSTKLAYQFNP
                     PLDPVTFAPLPPEVTAVRCANPDAPTDSCTLDFEQETDEPTWLIGLDYTPADDLLLYA
                     KYARGYRAGTIASDLSAPFNYIRPERVDAYELGSKFTFHGTVSGIFNAAVFFNDFENQ
                     QLQLGFNDNPADDIVVAPTAGPVNAGASEIYGVELEAAITPFERFTIEAGYTYLNTEI
                     TEMPVFIVPPSSPYVITGKQQEGDELALSPEHKYTIAARYTLPLDESMGEVTLGVMFI
                     HTDEQLSNYVDDGLIGSDFEGLSYLPETDIVNANLSWDSVGGSAFDLLVFGTNLTDEE
                     YFSFVPGLAEGAGFETAQLGLPRMYGVQLRYRFGN"
     gene            6319..7596
                     /locus_tag="PLW13_12015"
     CDS             6319..7596
                     /locus_tag="PLW13_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71149.1"
                     /translation="MTTQSSGERDYFSDHSVLLDPYGYFEEMRARGPVCQLDKRDILM
                     VTGYDEAIEVLRNTRDFSSSISVPGAAVPLPFDPVGDDISEQIEAHRDRMLGGDMVVT
                     YDDARHAAVRSLVSRLFTPSRLRANESFMHEFAEGLVRDAVANGRCEIVNEIATPYVT
                     LVIADLLGVPAQDRELFRKAIDAGPPPGNMDDEEKPTTTATLEYLGGFFYQYVQDRRA
                     NPRDDVLTELATATYPDGSLPDILEVVRLAVFLFAAGQDTSAKLLGNAMRRIADTPAL
                     QQQLRGNPGLIPSFLEEVLRLEGSTKATFRLARRKTRIGEFEVPAGKRVVVALAAANR
                     DPRRWDEPQEFRFDRPRIREHLAFGRGVHTCIGAPLARAEVRVILEHFLRHTSAISLS
                     EEKHGKPGNRRLDYEPSFIIRGLANLHVELAGK"
     gene            7603..>8108
                     /locus_tag="PLW13_12020"
     CDS             7603..>8108
                     /locus_tag="PLW13_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71150.1"
                     /translation="MTRAGQLMTGGLAPGHDLVFPARPENPDMRESTSIWLYEENGAF
                     GFPRLGIEAEASSWDDRRVQGNFAMAGGRVLNAAGTGAAHSPLGPDGRPTVLGAGPLA
                     LRCITPFRKWRVSFAGTAVDGTVDQQIAGTLDPDKRTAVRLDVDLHMVTPGLGAPLAP
                     PMAAAPIAA"
BASE COUNT         1331 a         2688 c         2771 g         1318 t
ORIGIN      
        1 gggggagatc tggtgccagg gtttctccga gcccaatgcc ggttctgacc tcgcctcgct
       61 gaaaacgcgc gcggagcggc gcggcgatcg ctacgtgatc aacggccaga agatctggtc
      121 gacgatggcg cagtacgcgg atcgctgcct gctgctggcg cgcagcggca ggtccgggcc
      181 cccgcaggcc ggcctcacct acctgttgct cgacatgcgc agcaagggcg tgtcggtgcg
      241 accgattgcg cagatcaccg gcgacgacga gttcgccgag atcttcttcg acgacgtgga
      301 agtgccggtt gaggatctcg tcggcgcgga gggcgagggc tgggcggtgg cgcaggccac
      361 gctggcctcg gagcgcggcc tcacgctggt ggagctgagc cagcgcatgc gctacgcgct
      421 gccgctgctc ggtgccgcga tgcgcgaggc ggggcgccag ggcgatgcgg cgctgcgccg
      481 cgacctgggg cgcgtgatgg cgaaggtcga cggcgcctgc gcgctggccg actggtacct
      541 gctgcggcgc atttccggcg aggaagcgcc gggcgatgcc tcgatggtca agctgaacta
      601 tgcccgcacg ctgcgcgaat tcacctcgct cgggctgcgc atcggcgggc tgcagagcca
      661 gtaccaccgc ggcttcatgc gcggcgccac gcaggaaacc ggcaactgga tggtcgattt
      721 catgaactcc tacaactggt cgatcgccgg cggcagcgac gagatccagc gcaacatcat
      781 cgccgagcgc ctgctgcgca tgccgcgcga gccgaagacg tggcaactgc cggaggagcg
      841 cgcatgaacg cggatctcct ggagttgcag caatcggtgc gacaggtgct ggccgggcgc
      901 gcggtggccg cggacgagca ggatacctgg ccgctcgtgg tcgaactggg ctggctgctg
      961 gcgggggtcc ccgaggagtt gggcgggctc ggccagggtc ttgcgggcgc ccgcgtgctg
     1021 cagatcgaac tgggccgccg gctcgcggcc gtgcccttcc tgccggccgc gctggcgctg
     1081 gacgccgtgt gccagtccgc gctggccgat cgcgcgcaat gggtcgagcg attgaccacg
     1141 ggtgaatacg tggcggcgcc gctggccgaa tccgcgctcg atctcgagcc ggccggcggc
     1201 ggcaccgcgc ggctctcggg gctggcctcg ggcgtgcagt cggcggatcg cgccagccac
     1261 gcgctggtgt ggacccgcgg cggcgactgc gtcgcgctgg cgccgctgga gcgcgcgggc
     1321 gtggagatcg tcgcgcgtcc gacctgggac cggacgcatc gcctgttcga cctgcgcttc
     1381 gcgggcctgg agctcgatga gacgctcgtg ctcgcgcgcg gtgcggcggc gcaggcgctg
     1441 gtgcgcaggc tgggcgcgct gcgcgatctc gggctcgcgg ccgacgccgt gggcggtgcc
     1501 aatgccctgc tggagctgac ggtggaacac ctgcaggtgc ggcgccagtt cggtcgtccg
     1561 ctggcgctgt tccaggcgct gaaacatcgc tgcgccgatc tgaagacgct gatcgcggcc
     1621 gccgaggccc tgctgctcga cagcctcggc cggctcggcg attccgtcgc ggatgccgaa
     1681 gccgaactga aggccgagac ggccaagtac ctcgcgagct ccacctacgc gaaggtggcc
     1741 gaggaagcgc tgcagctgca cgggggcata ggcatggcag ccgagcacca gtgccacctg
     1801 ttcctgaaac gcgcgctgct gaacgaacag ctgggccggc aggacgagcg ctatgcgctc
     1861 gacattgccg acagcctgct ggcaagattg gactgagcgg ccacggcgcc tgtcgacaac
     1921 ccaaccagaa ggaatagcta tccatgtttt ctgctgtcgt gatcaacaag ggcgaatccg
     1981 gccagtccgt cgagctcaca caggtcgatg aatcgcagct ccccgagggc gatgtcacga
     2041 tcgacgtcga gtattcgacc ctgaactaca aggacgggct cgcgatcacg ggcagctcgc
     2101 ccgtggtgcg caagttcccg atggtgccgg gcatcgacct cgcgggcgtc gtgcgcgaga
     2161 gcagccacgc cgactggaaa gcgggcgacc gcgtgatcct gaacggctgg ggcgtcggcg
     2221 agacgcactg gggcgggctg gcgcagaaag cgcgcctgaa gggcgagtgg ctggtgccgc
     2281 tgcccagcgc gttcagcagc cgccaggcga tggcgatcgg caccgccggc tacacggcca
     2341 gcctgtgcgt ggatgcgctg ctcgcccacg gcgtgcgccc cgagcagggc gagatcctgg
     2401 tcaccggcgc caccggtggc gtgggcagcg tggccgtcgc gctgctggtc aaggccggct
     2461 tcagcattgc cgccgcgacc ggcaaggccg ccgaggccga ttacctgaag cagctcgggg
     2521 ccagcacgat catcgatcgc gccgagctcg cggcacccgg caagccgctg cagaaggagc
     2581 gctgggccgg cgtggtcgat tgcgtcggca gccacacgct ggtcaatgcc tgcgcgcaga
     2641 cgcgctacgg cggcgcggtc gcggcctgcg gcctggccca gggcgcggat ttccccgcga
     2701 cggtgatgcc gttcatcctg cgtggcgtgt ccttgctggg cgtcgacagc gtgatggcgc
     2761 cgaaggctcg ccggctggtg gcttatgagc gactggcgcg cgatctcgat ggcgccgcac
     2821 tcgacgtcat tgccacggaa atcgggctgg ccgacgcgat cgcgacggcc gccgatttgc
     2881 tgcagggcaa ggtgcgcgga cgtgtcgtgg tgaacgtcaa caagtgagcg ttcgcgggcg
     2941 ccggatttcc ggcgttcgtc aggatgtgct ccgtcgccgc cctcccgggc ggcggcgggt
     3001 gcttccgcgc tccggaggcg ctccggcgcc ggcattcttg accggcggca tgctggggat
     3061 gatgaccagc tgatccagta tctccgccat tcgttgcacc gacggcggtt tgctctggcg
     3121 cagccaccac gcgaggatct ccaccgtcga tgccacggag aagaccacgc tgagatcgcc
     3181 cggcagccag gcgtgccggc tggatcgttc agcggcaatc tgccgggcct ggcggacgaa
     3241 ttcgtccttt agcatggcgg cggcgccgcc cgtcagcagc gccgaccaga gcttgcgata
     3301 ctcccagaca tagctgcaca gcgcctcggc cgacgcgcgg ctgtcgaccg tgtagagaat
     3361 tggctgggcc atcgcgagca gcttgctgat ctcgtgcgcc gcgaggtcgt gcagcagggc
     3421 ttccttgtcc gggtagtgcc ggaagaaggt ggcatagcca acaccggcgc gcgcggtgag
     3481 ttcccgcacg gtgacttgtt caaacggcct ttcttcgagc aacgacagca atgcctcgcg
     3541 tagcgcgggc cgcgagcgcg tgctccgctt tccgcttgct gtcgacgatt gcgcggcttt
     3601 tgccgcacgc ttttcatcac ctgccacgcc ttgtccccgg gcctcgcgca cgcgcttgac
     3661 gaaatccggc gcggctgtta ttggtacacc gtatatcaat aacagcggcg ctcccgcaag
     3721 ccgatacaca taacaggggc gggtccgccg tccgaacgac acccgagggg gaaacatgaa
     3781 ccgcgttgct gtccggaaaa ttctgcgtgg ctccagtctg gcggtattgg gggccgtctc
     3841 gcccgcatac gcgcaaggcg aggcgggcgc cggggaactc gagaacatca tcgtgacggc
     3901 caggcgggtc gaggagcgcg tgcaggacgt cccgatctcc atcacggtct tcaaccagca
     3961 gcaactcacg aaacagaatg tcgtgaactc cgctgacctc gcggccttca cgccgtcgct
     4021 gtcggcgaac agcaacttcg gcaacgagaa ttcgagcttc gcgatccgcg gcttcgtgca
     4081 ggacctgggc acgttgccgt cggtgggtgt ctatttcgcc gacgtggtgg ccccgcgcgg
     4141 ttcctccaac ggcagcttcc cggtcggcga tggcgcgggc cccggctcct tcttcgatct
     4201 ccagaacgtg caggtgctga agggaccgca aggcacgctg ttcgggcgca acaccaccgg
     4261 cggcgcggtg ctgctcgtgc cccagcgccc gacacacgag ctgggaggct atgtgcaggc
     4321 ctcggccggc aatctggaca tgcagcgcct gcagggtgta gtgaacgcgc cgctgggcga
     4381 caaggccgcg tttcgcctcg gcgtcgacaa gcagacgcgc gatggctacg tcgagaacac
     4441 cagcggcatc ggcccggacg atttcggcga tgtcgactac acggcctacc gggccagcct
     4501 gaccgtggag ctgacacccg atctcgagaa ctacgcgatc ttctcccgct ccgattcccg
     4561 cacgaacggt gtgttcggca agctggtggc cgccgacgcg gcggtgggcc tgggattctt
     4621 tgccgcgcag cagcttgccg accaggaggc cgacggcgac ggcttctacg acgcgcgaca
     4681 ggatctcgcc aacccgctgt ccgacctgca gcaatggcag ctcatcaaca ccacgacctg
     4741 gcaggtgcac gacgatctca cgttcaagaa catcatcagt tatgccgagc tgacccagga
     4801 tctgcgctcg gcgatgttcg gcaccgcctt cgtcacgccc gccctcggcg ccttgttgcc
     4861 cagctatcgc gtcggcttcg ccagcatcca gccgattccc gggggccata ccggtgacca
     4921 gtcgacgttc acggaagaaa tgcggctcga cggcgcgggc ttcgacaacg cgctgacctg
     4981 gcagacgggc gcctatgtcg agcgcagcga gccgctgagc gcggtgggat cgcagtcgcc
     5041 ggtggcgatt tcctgcaccg acgtcgccgc actggagtgc acggatgtgc tgggtttcct
     5101 gggcagcctg gccaccggca catacgcgcc ggccggtgcg atcaactaca ccagcggcaa
     5161 gaccgagttc aacaacgtcg gtctctacgc gcagggtact taccagttca gcgggaacct
     5221 cgccctgacc gctggcctgc gctacacctg ggatcgccag gagagcacca gcacgaagct
     5281 ggcctaccag ttcaatccgc cgctcgatcc ggtcaccttc gccccgctgc caccggaagt
     5341 caccgcggtg agatgcgcca acccggacgc gccgacggat agctgcacgc tcgatttcga
     5401 acaggaaacc gacgagccga catggctgat cgggctggat tacacgcccg ccgacgatct
     5461 gttgctgtat gccaagtacg cccgcggcta ccgggcgggc accattgcct cggacctcag
     5521 tgccccgttc aactacatcc gtccggagcg ggtcgacgcc tatgagctcg gctcgaagtt
     5581 cacgttccac ggcactgtca gcggcatctt caacgcggcg gttttcttca acgacttcga
     5641 gaaccagcaa ttgcagctgg gcttcaatga caaccccgcg gacgacatcg tcgttgcgcc
     5701 gacagccggg ccggtcaatg ccggcgcctc ggagatctat ggcgtcgaac tggaggcggc
     5761 gatcacgccg ttcgagcgtt tcacgatcga ggccggttac acctacctga acaccgagat
     5821 cacggagatg ccggtattca tcgtgccgcc gtcatcgccg tacgtcatca ccggcaaaca
     5881 gcaggagggc gatgagctgg cgctgtcgcc cgagcacaaa tacacgatcg cggcgcgcta
     5941 cacgctcccg ctcgacgaga gcatgggcga ggtgacgctg ggggtgatgt tcatccacac
     6001 cgatgagcaa ctcagcaact atgtcgatga cgggctcatc ggctccgact tcgagggcct
     6061 gagctacctt ccggagaccg acatcgtcaa tgccaacctg agctgggatt cggttggcgg
     6121 atccgcattc gacctgctgg tgttcggcac caacctcacg gacgaggaat acttcagctt
     6181 cgtccccgga ctcgcggagg gcgcgggttt cgaaaccgcc cagctcggcc tgccgcgcat
     6241 gtatggcgtg cagctgagat accggttcgg gaactgagct ccagattctt gccctcggcc
     6301 atgacacagg aaacatcgat gacgacacaa agctccgggg agcgcgatta tttttcggac
     6361 cattccgtcc tgctggatcc ctacgggtat ttcgaggaaa tgcgcgccag gggcccggtc
     6421 tgccagctcg acaagcgcga catcctgatg gtgaccgggt acgacgaggc gatcgaggtg
     6481 ctcaggaata cccgggactt ctcgtcgtcc atctcggtgc ccggcgcggc cgtaccgctg
     6541 ccattcgatc cggtcggcga cgacatctcg gagcagatcg aggcgcatcg cgaccgcatg
     6601 ctgggtggcg acatggtggt gacctatgac gatgcccggc atgccgccgt gcgctcgctg
     6661 gtcagccgcc tgttcactcc ctcgcgactc agggcaaacg aatcgttcat gcacgaattt
     6721 gccgaagggc tcgtgcgcga cgctgtcgcg aacggacgct gcgagatcgt caacgaaatc
     6781 gcgacaccct acgtgacgct ggtgatcgcc gatctgctcg gggtcccggc gcaggaccgc
     6841 gagctgtttc gcaaggccat cgacgcgggg ccgccgcccg gcaacatgga tgacgaggaa
     6901 aagcccacga ccaccgcgac gctcgagtac ctcgggggtt tcttctacca gtacgtccag
     6961 gaccggcgcg ccaatccgcg ggacgatgtc ctgacagagc tcgcgaccgc gacctacccg
     7021 gatggctcgc tgcccgacat actggaggtg gtccggcttg ccgtgttcct gtttgccgcg
     7081 gggcaggaca ccagcgcgaa gctgctaggc aacgcgatgc gccgcatagc cgacacgccc
     7141 gctttgcagc agcagctgcg cggcaatccc gggttgatcc cgtcattcct ggaggaagtc
     7201 ctgcgcctcg aaggctccac caaggccacc ttccggctgg cgcgcaggaa gaccaggatt
     7261 ggcgagttcg aggttccggc gggcaagcgg gtggtggtcg cgctcgcggc ggccaaccgc
     7321 gacccgcgcc gctgggatga accgcaggag ttccgcttcg atcgtccgcg gatcagggag
     7381 cacctggcat tcggccgggg cgtgcatacc tgcatcgggg caccgctggc gcgcgcggag
     7441 gtccgggtca tcctggagca cttcctgcgg cacacctccg cgataagcct gtccgaggag
     7501 aagcatggca agcccggcaa ccgccgcctc gactacgagc cgagcttcat cattcgtggc
     7561 ctggcgaatc tgcacgtcga gcttgccggg aaatgacggg cgatgacacg tgccgggcaa
     7621 ttgatgacgg gcggcctggc gcccgggcat gacctggtat tcccggctcg cccggagaat
     7681 ccggacatgc gggaaagcac ctcgatctgg ctctacgagg aaaatggcgc ctttggcttt
     7741 ccccgtctcg gtatcgaggc ggaggcctcc agctgggatg atcgccgcgt ccagggcaat
     7801 ttcgccatgg cgggcggacg cgtactgaac gctgccggca ccggggctgc ccattcgccg
     7861 ctgggtcccg atgggcggcc gacggttctc ggcgccggac ccctggcgtt gcgctgcatc
     7921 accccgttcc gcaaatggcg cgtcagcttc gcgggaaccg cggtcgatgg cacggtcgat
     7981 cagcagattg ccggcacgct ggatcccgac aagcgcaccg cggtacgcct ggacgtcgat
     8041 ctgcacatgg tcacgccggg gctcggtgcg ccgctggcac ctcccatggc tgcggccccg
     8101 atcgccgc
//
LOCUS       DAORUQ010000147         8056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_9966_length_8056_cov_6.740532, whole genome shotgun sequence.
ACCESSION   DAORUQ010000147 DAORUQ010000000
VERSION     DAORUQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9966_length_8056_cov_6.740532"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLW13_12025"
     CDS             complement(<1..1253)
                     /locus_tag="PLW13_12025"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQY71151.1"
                     /translation="MPGTSRARANNQRVLSVIMGGGAGTRLYPLTRERSKPAVPLAGK
                     YRLVDIPISNCLNSGLRRVYVLTQFNSASLHRHIAQSYLLDHFGGGFVQILAAEQTPT
                     TQSWYQGTADAVRKNLQHLLSHDFDHVLILSGDQLYRMDFRLLIEQHVDTGADLTVAT
                     LAIGREAAPALGIMQLGEGNRVARFVEKPQDPQLLDSLRLDAAAAAALGLAASGERFL
                     ASMGIYVFRRQVLLDLLDNTLTDFGKHIIPGAIDSHRVHAFVFDGYWEDIGTIRSFFE
                     ANLDTTRELPQFDFFNMAAPIFTHPRFLPASKINGAQIDHAVISDGCIINPSRISHSL
                     IGIRSIVGAGSTLHRVVSFGGDYYESDASIREAAARGWPRIGIGEHTHISNAIIDKNA
                     RIGSHVVISPQGKPENFDHDLYCIR"
     gene            complement(1365..1700)
                     /locus_tag="PLW13_12030"
     CDS             complement(1365..1700)
                     /locus_tag="PLW13_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71152.1"
                     /translation="MKTIYGVVLTAVVAGMASTVSAASMSVAEAATACKAQAASQYAS
                     GERPARVKFKGVYGGNDSRKVRMQVLPADGGKAFLAICEVNGRSGEVIGLAPRARGTR
                     PELAMSAAR"
     gene            1816..2727
                     /locus_tag="PLW13_12035"
     CDS             1816..2727
                     /locus_tag="PLW13_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY71153.1"
                     /translation="MQSINWDDLRYCLAVAKEGSVTAAARKLAVNHTTVSRRISALEQ
                     ELNVTLFDRSTTGWLITPVGETILGSAEHMADEVNSIRRLVQADRQELSGKLRVTSVD
                     ICIQRLLLPPLRAFARQYPDIDIELIASTEVIDLSVHDADIAFRTTNQPPANVVGKHI
                     ADFAYAVYATPELHEQFRADPRKVSAITWNAMASASTAPAWLAKCFPGMPMRYRVNSL
                     TIAFDLARQGMGFTLLPCAIGDIAPELRRVPVDYREPPVGFWVLSHIDLRTTARIRIF
                     RDFMLEAIAPYVPLIEGERENAWQASR"
     gene            complement(2717..3631)
                     /gene="nhaR"
                     /locus_tag="PLW13_12040"
     CDS             complement(2717..3631)
                     /gene="nhaR"
                     /locus_tag="PLW13_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HQY71154.1"
                     /translation="MFNYRQLHYFQAVAKSGGIARAAEQLHLTPQTLSGQIGQLEDRL
                     GVELFRRAGRRLELTEAGRLALSYADEIFQVGGELEEALRNWHAERPFLFRVGIADVV
                     PKSIAYRLLAPALALPEQVRMVCREDKLERLLAELAVHRLDMVVADSPMPPGMDVKGY
                     SHRLGDCGTTFLAAPALAATLAGEFPQSLDRAPLLIPGEDSALRAPLLRWLHAANIHP
                     RLVGEFDDGALMKAFGQAGAGVFAMPSAIAAEVARQYGVVAVGATEAVRERFFLITVE
                     RRISHPAARVVTEAARSGLFTAAATLNG"
     gene            3747..4094
                     /locus_tag="PLW13_12045"
     CDS             3747..4094
                     /locus_tag="PLW13_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQY71155.1"
                     /translation="MQVDIQCRGFELTGSLREHVRKRLACNLPAGGTAIQRVTVRLSD
                     INGPRGGEDKRCHIALRLKGLPRLVIEDTEADLYVAIDRAAERAGRTLVRRLARQLRP
                     APGFPEAAPAVDA"
     gene            4094..4603
                     /gene="purE"
                     /locus_tag="PLW13_12050"
     CDS             4094..4603
                     /gene="purE"
                     /locus_tag="PLW13_12050"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQY71156.1"
                     /translation="MAPAQPLVGIVMGSDSDWPVMQAAAQVLEEFGVAHEARVVSAHR
                     TPDLLFEYAAAARGRGLRAIIAGAGGAAHLPGMLAAKTPLPVLGVPVPSRHLKGLDSL
                     LSIVQMPRGIPVATFAIGEAGAANAALFALALLAANGTADAPLAHALDAFRARQRDRA
                     LGMRLPVGT"
     gene            4600..5760
                     /locus_tag="PLW13_12055"
     CDS             4600..5760
                     /locus_tag="PLW13_12055"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQY71157.1"
                     /translation="MILPPATLGVLGGGQLGRYFVLAARELGYRTLVLDPDPHGIAGA
                     VADEHVVAAYDDAVALERVAGRCAAVTTEFESVPAAALARLERAVPVRPGAATVAICQ
                     DRSAEKGFLRRHDLPHAPYADILREEDCEHCAPTLLPGILKLARCGYDGKGQARVADR
                     AGLRAAFRDFGTRACVLERLLPLDGELSVVLARDAAGNTRCFPATANHHRNGILDRSI
                     APAPEGALTDAAPRAQAIAAVIAARMDYVGTLGVEFFLCGGELLVNEIAPRPHNSGHY
                     TLDACVTNQFEQQVRTLCGLPLGDTDAHCAAVCVNLLGDLWYPDGASVPREPDWRRLL
                     ERPGLRLHLYGKDQPRPGRKMGHFTVIGRDRHEVLLAAMAARAAIGIRDDRG"
     gene            5774..6745
                     /locus_tag="PLW13_12060"
     CDS             5774..6745
                     /locus_tag="PLW13_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQY71158.1"
                     /translation="MNPDTAVSIGEPWMWAAFVGFVLVMLALDLFVFGGRQAHKVSVK
                     EAAAWSMVWVSLALAFNAGLWWHLNGTVGEEIADRKALEFLTGYLIEKSLSVDNVFVF
                     LLIFSSFHVAAEYQRRVLLYGVLGAIVMRAVMILAGAWLVREFHWVLYLFGVFLVITG
                     LRMLVMAEKEPDLEKNPLLRFARRHLRITEDYHGERFTVVKDGVRWFTPLFLVLLLVE
                     ASDLVFAVDSIPAIFAITTDPFIVFTSNIFAILGLRALYFLLADVAGRFHLLKYGLAM
                     VLTFIGTKMLIAPWYHVPVAASLAIVAVLIGASVIASLVATRGRPAA"
     gene            6742..7680
                     /locus_tag="PLW13_12065"
     CDS             6742..7680
                     /locus_tag="PLW13_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HQY71159.1"
                     /translation="MIDVVVMFFLFGLAAGLLRSELKLPASLYDTLSLFLLLAIGLKG
                     GEGLAQQALGPLVPQLLAVIALGVLQTLIAFAILRVLPGFGRADAAATAAHYGSVSVA
                     TFAVGVNWLVSREIAFEPQMTIFLAVMEVPAIVVGIVLARGIGRRTDWGALAHEAFLG
                     KGVTLLLGGMAIGWAAGPEGLAPVKGFFYELFKGALALFLLEMGLIVSRQVGELRRSG
                     AALLGFGLLMPLLSAGLGLGCGLLLGLSTGGLTLLATLAASASYIAVPAAMRLAVPEA
                     RPALSLAAVLGVTFPLNILAGIPLYHRLALQLTGSA"
     gene            7680..7988
                     /locus_tag="PLW13_12070"
     CDS             7680..7988
                     /locus_tag="PLW13_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY71160.1"
                     /translation="MTLPTEKRTLLTVITEATIEQALLREFDRLGVRGYTVWDARGRG
                     NRGMRDGSWEESANIRIEMVCPRSQAEAVLGYLQARYYADYAMVGFLQDVEVLRPGKF
                     "
BASE COUNT         1185 a         2773 c         2760 g         1338 t
ORIGIN      
        1 tcgcggatgc agtacagatc gtggtcgaaa ttctccggct tgccctgcgg cgagatcacg
       61 acgtggctgc cgatgcgcgc gttcttgtcg atgatcgcat tgctgatgtg cgtgtgctcg
      121 ccgatgccga tgcgcggcca gccgcgcgcc gcggcttcgc gtatcgaggc gtcggactcg
      181 tagtagtcgc cgccgaaact caccacgcga tgcagcgtcg atcccgcgcc cacgatgctg
      241 cggatcccga tcaggctgtg cgagatacgc gaggggttga tgatgcatcc gtcggagatc
      301 acggcgtggt cgatctgcgc gccgttgatc ttcgaggccg gcaggaagcg cgggtgggtg
      361 aagatcggcg ccgccatgtt gaagaagtcg aattgcggca gctcgcgcgt ggtgtcgagg
      421 ttggcctcga agaacgagcg gatcgtgccg atgtcctccc agtagccgtc gaacacgaag
      481 gcgtgcacgc gatggctgtc tatcgcgccc ggaatgatgt gcttgccgaa atccgtgagc
      541 gtgttgtcca gcaggtcgag cagcacctgg cgcctgaaca cgtaaatccc catcgaggcg
      601 agaaagcgtt cgccgctggc tgcgagcccg agcgcggcgg cggccgccgc atcgaggcgc
      661 agcgagtcca gcagttgcgg gtcctgcggt ttctcgacga agcgcgcgac gcggttgccc
      721 tcgccaagct gcatgatgcc gagcgcggga gccgcctcgc gcccgatcgc gagcgtcgcg
      781 accgtgagat cggcgccggt gtcgacgtgc tgctcgatga gcaggcggaa atccatgcgg
      841 tagagctggt cgccgctgag gatcagcacg tgatcgaagt cgtggctcag caggtgctgc
      901 aggttcttgc gcaccgcgtc ggccgtgccc tggtaccagc tctgcgtggt cggggtctgc
      961 tccgcggcga ggatctgcac gaaaccgccg ccgaaatgat cgagcaggta ggactgggcg
     1021 atgtgccggt gcagcgaggc cgagttgaac tgtgtcagca cgtagacgcg gcgcaggccg
     1081 gaattgaggc agttggagat cgggatgtcc accaaccggt acttgccggc cagcggtacc
     1141 gcgggcttgg atcgttcgcg cgtcagcgga tagagccgcg tgccggcgcc tccgcccatg
     1201 atcacgctga gtacgcgctg gttgttggct ctggctcgtg aagttccggg catgaatcgg
     1261 cgccgttgca ggggcaaggc gccggattat tgcatagcgg gtcgatgtgt cgtgcgcgct
     1321 gctgcgatgc gggccggacc gcagcccggc ccaagggtcg gttctcagcg cgcggcgctc
     1381 attgccagct cgggccgcgt gccgcgggcg cgcggggcca gcccgatcac ttcaccactg
     1441 cggccgttca cctcgcagat cgccaggaaa gccttgccgc catcggccgg cagtacctgc
     1501 atgcgcacct tgcgactgtc gttgccacca tagacaccct tgaacttgac ccgcgcgggg
     1561 cgttcgcccg atgcgtactg cgatgccgcc tgcgccttgc acgccgtggc cgcctcggcc
     1621 accgacatcg atgccgccga taccgtcgag gccatgcctg cgaccactgc cgtcaatacc
     1681 acgccgtaga tcgtcttcat tgccgttctc ctcatgtacc gttggggaat ccgtgcttgt
     1741 tcaccgacac ggctccactg tagtctgtgc tttggcgcac gagaatagat gtttgagcac
     1801 attcgaggtg caaatatgca gagcatcaat tgggacgacc tgcgctattg cctcgcggtc
     1861 gcaaaagagg gctcggtcac ggccgccgcg cgcaagctgg cggtcaacca caccacggtg
     1921 tcgcggcgca tcagcgcgct ggagcaggaa ctgaacgtca ccctgttcga ccgctccacc
     1981 acgggctggc tgatcacgcc ggtcggcgag accatcctcg gatcggccga gcacatggcc
     2041 gacgaggtca acagcatccg ccgcctggtg caggcggacc gacaggagct gagcggcaag
     2101 ctgcgcgtca cctcggtcga catctgcatc cagcgcctgc tgctgccgcc gctgcgcgcc
     2161 ttcgcgcgcc agtatcccga catcgacatc gagctgatcg cctccaccga ggtgatcgac
     2221 ctctcggtgc acgacgccga catcgcgttt cgtaccacga accagccgcc cgcgaacgtg
     2281 gtcggcaagc acatcgccga cttcgcctat gccgtctacg ccacacccga actgcacgag
     2341 cagttccgcg cggacccgcg caaggtcagt gcgatcacct ggaacgcgat ggcgtcggcg
     2401 agcaccgcgc cggcctggct cgcgaagtgc tttcccggga tgcccatgcg ctatcgcgtg
     2461 aattcgctga ccatcgcctt cgacctggcg cgccagggca tgggcttcac gctgctgccc
     2521 tgcgccatcg gcgacatcgc gcccgaactg cgtcgcgtcc ccgtcgacta ccgcgagccg
     2581 ccggtcggct tctgggtgct gtcgcacatc gacctgcgca cgacggcgcg catccgcatc
     2641 ttccgcgact tcatgctgga ggcgatcgcg ccttacgtgc cgttgatcga gggcgaacgc
     2701 gagaacgcct ggcaggctag ccgttgagcg tcgccgcggc ggtgaacagc ccgctgcgcg
     2761 ccgcctcggt cacgacgcgc gccgccgggt ggctgatgcg acgctccacc gtgatcagga
     2821 agaagcgctc gcgcaccgcc tcggtggcgc ccaccgccac cacgccgtat tggcgcgcca
     2881 cctcggcggc aattgcgctc ggcatggcga acacccccgc gccggcctgg ccgaaggcct
     2941 tcatcagcgc gccgtcgtcg aactccccga ccaggcgcgg gtggatgttc gcggcgtgca
     3001 accagcgcag cagcggcgcg cgcaacgcgc tgtcctcgcc cgggatcagc agcggcgcgc
     3061 gatcgagcga ttgcggaaac tcccccgcga gagtggcggc cagcgcgggc gccgcgagga
     3121 aggtcgtgcc gcaatccccg agccggtggc tgtaaccctt cacgtccatg ccgggcggca
     3181 tcgggctgtc ggccaccacc atgtccaggc gatgcaccgc cagctccgcc agcagacgct
     3241 ccagcttgtc ctcgcggcac accatgcgca cctgctcggg tagcgcgagc gcgggcgcaa
     3301 gaaggcggta ggcgatcgat ttcggcacca cgtcggcaat gcccacgcga aacaggaagg
     3361 ggcgctcggc gtgccagttg cgaagcgcct cctccagttc gccaccgacc tggaagatct
     3421 cgtcggcata ggacagcgca aggcgcccgg cttcggtgag ttcgagccgg cgcccggcac
     3481 ggcgaaacaa ctccacgccc agccggtcct cgagctgccc gatctggccc gacagcgtct
     3541 gcggcgtgag gtgcagttgc tcggctgcgc gcgcgatgcc gccggacttg gccacggcct
     3601 ggaagtaatg cagttggcga tagttgaaca cggtccaccc atacttcgtt aaaaacgatc
     3661 aatgtgaaaa taataaacga attttattga tgcaatcgat ctcttagcat ggcctcccga
     3721 tgcatgaacc tgcgaggaga gaagcgatgc aagtggatat ccagtgccgc ggtttcgaac
     3781 tgacagggag cctgcgcgag cacgtgcgca agcgcctggc ctgcaacctt cccgccggcg
     3841 ggaccgcgat ccagcgcgtg acggtgcgac tgtccgatat caacggtccg cggggcggcg
     3901 aggacaagcg ctgccacatc gccttgcgcc tgaagggcct gccgcgcctg gtgatcgagg
     3961 ataccgaggc ggacctctac gtggcgatcg accgcgccgc cgagcgtgcc gggcgcacgc
     4021 tggtgcgtcg cctggcgcgc cagttgcgcc cggcgcccgg atttccggag gcggcgccgg
     4081 cggtggacgc gtgatggccc cggcacaacc cctggtcggc atcgtgatgg gatcggattc
     4141 ggactggccc gtcatgcagg cggccgcgca ggtgctggag gagttcggtg tcgcacacga
     4201 ggcgcgcgtc gtctcggcgc atcgcacccc cgacctgctg ttcgaatacg ccgccgcggc
     4261 acgcgggcgc gggttgcgcg ccatcatcgc gggcgccggc ggcgcggccc acctgcccgg
     4321 catgctggcc gcgaaaacgc cgctgccggt gctgggggtg cccgttccct ccaggcacct
     4381 gaagggactg gattcgctgc tctccatcgt gcagatgccc aggggcatcc cggtggcgac
     4441 cttcgcgatc ggcgaggccg gcgctgcgaa tgccgcgctg ttcgcgctcg ccctgctcgc
     4501 cgccaacggc acggccgacg cgccgctggc gcacgcgctc gatgccttcc gggcccgcca
     4561 gcgcgatcgc gcgctcggca tgcgcctgcc ggtgggcaca tgatcctgcc gcccgcgacg
     4621 ctcggcgtgc tcggcggagg gcaactcggg cgctacttcg tgctggccgc acgcgagctg
     4681 ggttaccgca ccctggtgct ggacccggac ccgcacggca tcgccggggc tgtcgccgac
     4741 gaacacgtgg tcgcggccta cgacgacgcg gtggcgctgg agcgggtcgc cgggcgctgc
     4801 gccgccgtca ccacggaatt cgagagcgtt cccgccgccg cgctcgcacg actggaacgc
     4861 gccgtgcccg tgcgcccggg cgccgcgaca gtcgcgatct gccaggaccg ctcggccgag
     4921 aagggcttcc tgcgccgcca cgacctgccg cacgccccct atgccgacat cctccgcgaa
     4981 gaggactgcg agcactgcgc gccgacactg ctaccgggca tcctgaagct ggcgcgctgc
     5041 ggctatgacg gcaagggcca ggcccgcgtc gcggatcgcg ccgggttgcg cgcggccttc
     5101 cgcgatttcg gcacccgggc ctgcgtgctc gaacggctcc tgccgctcga cggcgagctc
     5161 tccgtggtgc tggcgcgcga cgcggcgggc aacacgcgct gcttcccggc aacggcgaac
     5221 catcaccgca acggcatcct cgaccgctcc atcgcacccg cgcccgaggg tgcgctcacc
     5281 gacgcggccc cgcgcgccca ggcgatcgcc gccgtcatcg ccgcgcgcat ggattacgtg
     5341 ggcacgctgg gcgtcgagtt cttcctttgc ggcggcgaac tgctggtgaa cgagatcgcg
     5401 ccgcgcccgc acaactccgg ccattacacg ctcgacgcct gcgtcaccaa ccagttcgag
     5461 cagcaggtgc gcacgctctg cggcctgccg ctgggcgaca ccgacgcgca ctgtgccgcc
     5521 gtctgcgtga acctgctagg ggacctgtgg tacccggacg gcgcgagcgt gccgcgcgag
     5581 ccggactggc gacggctgct cgaacgcccc ggcctgaggc tgcacctcta cggcaaggac
     5641 cagccgcggc ccggccgcaa gatggggcac ttcaccgtga tcggacgcga ccggcacgag
     5701 gtcctcctcg ccgcaatggc ggcacgcgcc gccatcggca tccgcgacga tcgcggctga
     5761 cgaggacatg gccatgaacc cggacaccgc ggtatcgatc ggcgaaccct ggatgtgggc
     5821 ggccttcgtc ggcttcgtgc tcgtgatgct ggcgctcgac ctgttcgtgt tcggcggacg
     5881 gcaggcgcac aaggtcagcg tgaaggaggc ggcggcctgg tccatggtct gggtcagcct
     5941 ggcgctcgcg ttcaacgcgg gactgtggtg gcacctgaac ggcacggtcg gcgaggagat
     6001 cgcggaccgc aaggcgctcg aattcctcac cggctacctg atcgagaagt cgctctcggt
     6061 cgacaacgtc ttcgtgttcc tgctgatctt ctcctcgttc cacgtggccg ccgagtacca
     6121 gcggcgcgtg ctgctctacg gcgtgctcgg cgccatcgtc atgcgcgccg tcatgatcct
     6181 cgccggcgcc tggctggttc gggagttcca ctgggtgctg tacctcttcg gtgtcttcct
     6241 ggtgatcacg gggctgcgca tgctggtgat ggccgagaag gagcccgacc tggaaaagaa
     6301 cccgttgttg cgcttcgcgc gccggcacct gcgcatcacc gaggactacc acggcgagcg
     6361 cttcaccgtg gtgaaggacg gcgtgcgctg gttcacgccg ctgttcctgg tgctgctgct
     6421 ggtcgaggcc tctgacctgg tgttcgcggt cgactcgatc ccggcgatct tcgcgatcac
     6481 caccgatccc ttcatcgtgt tcacctcgaa catcttcgcc atcctcggct tgcgggcgct
     6541 ctacttcctg ctcgccgacg tcgcggggcg tttccacctg ctcaagtacg gcctcgcgat
     6601 ggtgctgacc ttcatcggca ccaagatgct gatcgcgccc tggtatcacg tgccggtggc
     6661 cgcctcgctg gcgatcgtcg cggtgctgat cggcgccagc gtgatcgcga gcctggtggc
     6721 cacgcggggc aggcccgccg catgatcgat gtggtggtga tgttcttcct gttcggcctc
     6781 gcggccggat tgctgcgctc ggagctgaag ctgccggcct cgctctacga cacgctgtcg
     6841 ctgttcctgc tgctcgcgat cgggctcaag ggcggcgagg gactggcgca gcaggcgctc
     6901 ggcccgctcg tgccgcagtt gctcgcggtg atcgcgctcg gggtgctgca gaccctgatc
     6961 gcattcgcga tcctgcgcgt cctgccgggc ttcgggcgcg ccgacgccgc cgccaccgcc
     7021 gcgcactacg gctcggtcag cgtggcgacg ttcgcggtcg gggtgaactg gctggtgtcg
     7081 cgcgagatcg ccttcgagcc gcagatgacc atcttcctcg cggtgatgga ggtgccggcg
     7141 atcgtggtcg gcatcgtgct cgcgcgcggc atcggccgcc gcaccgactg gggcgcgctg
     7201 gcacacgagg cctttctcgg caagggcgtc acgctgctgc tgggcggcat ggcgatcggc
     7261 tgggcggcag ggcccgaagg gctcgcgccg gtcaagggtt tcttctacga gctgttcaag
     7321 ggcgcgctgg ccctgttcct gctcgagatg gggctgatcg tgagccgcca ggtcggcgaa
     7381 ctgcgccgca gcggggcggc attgctcggc ttcggactgt tgatgccact gctgtcggca
     7441 gggctcggcc tgggctgcgg cttgctgctg gggctgtcga cgggcgggct gacgctgctc
     7501 gcgacgctcg cggccagcgc ctcgtacatc gcggtgcctg cggcgatgcg cctcgcggtg
     7561 ccggaggcca ggccggcgct ttcgctggcc gccgtgctcg gggtaacctt tccgctcaac
     7621 atcctggccg gcataccgct gtatcaccgg ctggccctgc aactcacggg atccgcttga
     7681 tgacgctgcc cacggaaaaa cgcaccctgc tcacggtgat caccgaggcg acgatcgagc
     7741 aggcgctgct gcgcgaattc gaccggctcg gcgtgcgcgg ctacacggtg tgggacgctc
     7801 gcgggcgcgg caaccgcggc atgcgcgacg gctcctggga ggagtcggcc aatatccgca
     7861 tcgagatggt ctgtccgcgc tcgcaggccg aggcggtgct cggctacctc caggcgcgct
     7921 actacgccga ctacgcgatg gtaggcttcc tgcaggacgt cgaggtcctg cgtcccggaa
     7981 agttctgagt cggtcagcgc ctgcaagcac tcccggcgcg cgtgacctcc acggcgcgac
     8041 gaggtaacag accgcg
//
LOCUS       DAORUQ010000148         7947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10164_length_7947_cov_7.692980, whole genome shotgun sequence.
ACCESSION   DAORUQ010000148 DAORUQ010000000
VERSION     DAORUQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10164_length_7947_cov_7.692980"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..631
                     /locus_tag="PLW13_12075"
     CDS             347..631
                     /locus_tag="PLW13_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71161.1"
                     /translation="MELIVSHPNLHPVWAKVLGLQAEPARATHDTSSASPLAAAIAAM
                     TDRRGDSEGWQATHRPQRPGAPVAMKKRLGPGLWISTVVKPGDAGYPTHS"
     gene            655..1485
                     /locus_tag="PLW13_12080"
     CDS             655..1485
                     /locus_tag="PLW13_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71162.1"
                     /translation="MRLNPFSKKTTTGYYDRLKAECADLALQVQEAKAAAEDAKADYE
                     AKVQASRDMEVRYADKHFLTEQESQARRERDDAYNRAEELRRKAWELEHKHNSLRQAV
                     EAPARLEAAKQTIEHLSKERAALRDEVVKTEALIAKLRRRVEDLEQRIARETQAASRS
                     MVDADGEFVMPEALTRLDVELRVTRATLAERQTKLAELKDALELIPDQVREAQRTYGS
                     ARAVVAEIELQEQLPNWLEVIALAALSASSDARKYVIEIPREVAEAARAKLAADMPKA
                     "
     gene            1655..2104
                     /locus_tag="PLW13_12085"
     CDS             1655..2104
                     /locus_tag="PLW13_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71163.1"
                     /translation="MQVEDVEGGCNTAALLPIGRDTSVVELMVMGWFGAGHEVLWIPI
                     IEVGPGRDGAEQARVDAEQIGEPRVLRALADLGGLPQGIKGWLFPAHRDDTCIVACRE
                     DGATVTFGSLTHPITGRTLTFRTDTESGLLDLRRLNAATAASGQSAV"
     gene            2164..2601
                     /locus_tag="PLW13_12090"
     CDS             2164..2601
                     /locus_tag="PLW13_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HQY71164.1"
                     /translation="MTQPSLAARLAALPTLPMPQLWRLWDEHFPRRPQRVNRRYLEAR
                     LAYRVQEAALGGLPRVIKEHLADCGERHSKLKTGRGPELRLMPGTVLVREWDAREYRV
                     KVTDDRRYELDGQSYKSLSAVARAITGAHWSGPAFFGLKGTRK"
     gene            2598..3953
                     /locus_tag="PLW13_12095"
     CDS             2598..3953
                     /locus_tag="PLW13_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQY71165.1"
                     /translation="MNPVAPKRCAVYARVSSDERLAQSFNSIDAQREAGAAFVASQRA
                     EGWIVTGDDYLDPGYSGGNMDRPGLKRLMADIEAGRVDTVIVYKIDRLTRSLADFARL
                     VEVFDRNGVSFVSVTQQFNTTTSMGRLTLNILLSFAQFEREVTGERIRDKIAASKAKG
                     MWMGGVVPLGYRVDARRLLVEPVEAENVRRIFTRFAECRSTTDLVRWLAAQGIASRRG
                     TPFSKQALWKLLNNRIYLGEIVHKGQVFRGQHEAIIDTSLWDAVQAVFAVNHNERRHA
                     TWRKRGPDFLLTGLVVTEQGERLQPSFTTKGRTGQHYRYYVPKSVQAFGTKRAGHAVG
                     RLAAEPLEELVLAQIHAALQTPERIQGAWDAVRAQGAALTEPEVVLPMLHNFAGVWQA
                     LYPAEQQRIARLLIESVVVSEQAVSIVWRDAGWAELAREMRPDGIGAELLELEAEGAA
                     V"
     gene            3950..4429
                     /locus_tag="PLW13_12100"
     CDS             3950..4429
                     /locus_tag="PLW13_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase resolvase"
                     /protein_id="HQY71166.1"
                     /translation="MTKVLQTGAAQAGPKLTTVIPVRIKRSGGRRWMIPAAGGADGGA
                     EAPKHEMPVLTALSRAFHWQRLLDEGRVASGSEIARREGLHPTTVNELLRLTLLSPAV
                     VRALLAGKQPRTLSLIWLKNNEVPRSWDDQERLFGRMDGFESAPVAGVDREPPRASR"
     gene            complement(4618..4693)
                     /locus_tag="PLW13_12105"
     tRNA            complement(4618..4693)
                     /locus_tag="PLW13_12105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4658..4660),aa:Ala,seq:cgc)
     gene            complement(4718..5080)
                     /locus_tag="PLW13_12110"
     CDS             complement(4718..5080)
                     /locus_tag="PLW13_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HQY71167.1"
                     /translation="MRERRMGIDILSDDDGDDPILSVVNIVDVFLVIIAVLLIAVIEN
                     PLNPFATQDAVVIRNPGKPDMAMLIRQGNELKEYRSSGQIGEGEGAKAGTAYRLKNGT
                     MVYVPETDGAAAGGAEAR"
     gene            complement(5073..5597)
                     /locus_tag="PLW13_12115"
     CDS             complement(5073..5597)
                     /locus_tag="PLW13_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY71168.1"
                     /translation="MNAIENFLYEVSRFFLTPVLVLLSLMFVYALFCLGMLIFESALR
                     AARGQGRRPLAQWLRRRPGASPEALELQVLRQLEMQRIVSRIAPMLGLVATMIPMGPA
                     LIAVANGNTHGMAQNLVVAFAAVIVALLSAAMTFVVHSVRRRWLLEELEGLLQGRADS
                     FAAAKGETQGAAYA"
     gene            complement(5649..>7947)
                     /locus_tag="PLW13_12120"
     CDS             complement(5649..>7947)
                     /locus_tag="PLW13_12120"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HQY71169.1"
                     /translation="VGNTPVVYPYIVDNIGEAVHVKRRGRGVIVSHQTPAFAPAGLSS
                     DFVRIGDLIREYASLDQGLVKDNNRGLIIAQAVKMNIHRDMGWTVADLERDFEGFLRD
                     IEDYLEELGASMQPLGLHTLGRDAERAHLVSNVMQMLGQPLYERTGAGSARAAFAGDY
                     RQLQQSAPYRFVDEVVFADGPLDGIADLELRRLAQRGRQFRDALRADREIDGVLRGLA
                     ARWIDPSYGGDPIRNPDALPTGRNMYGFDPSRIPTRAAWEAGRQAVQQLIERHREQQG
                     RFPEKLAFSMWSTESMRHLGMLEAQVLYAMGARPVWDEGGRVTGVEPIPQVELGRPRI
                     DAVISLTGLYRDQFPNVIERFNEAVVMLSALDEPAQQNFVRANTERIRAQLSQRGVKP
                     EQAREFALTRVFGNESGDYSTRLTDATLASDKWEAGDGRLEQLYLSRMSWAYGPDPAN
                     WSRKLHDEGGAEINAYAEHLRGTSAAVFSRSSNLRGLLDTDHPFEYLGGISMAVKHLD
                     GRNPQLYISNMRDPRRAKLESASRFMATELRSVYQHPNWVAEMQKEGYAGTLQMLNAV
                     NNFWGWQVMDRNVVRDDQWQEFHEVYLKDRYRLGMRKWFENANPTALAQIAERMIEAI
                     RKDYWKADERTRRELVRVYAELAKKHDVYTPNEAFRAYVVEAARGFGVEGVKPRVPAQ
                     SAPPAPRPRASPEPRPAEAAPPPPAPRPPEIVRGQELREVRNEARLERLVWIYAWMIG
                     LVIVGGAGFQAWVTWRDRRRTTLPA"
BASE COUNT         1301 a         2757 c         2697 g         1192 t
ORIGIN      
        1 cgacaagatg ggtcctggcc gcgtcatccc gatggccgag atctaccgca agggtccgtc
       61 ggcgatccgc agtgccggtg cggcgcgcaa ggtgctccaa gtgctcgtgc agcacggctg
      121 ggtggcgcca gccgcgcacc ccacggccaa agaagccttc cagctggtgg cggtgtgatg
      181 aacccgccca cttccgtccc cgcgccagcg aattctgcga atgacgcgaa agaccgcgtc
      241 agcactcgcg tctcgcgttc gcactgccgt gcgaggcctg cgaatccgtc cgcgcacggt
      301 taccccgttc acgccacgcc acccggcatc gagggtggcc ttcgagatgg agctcatcgt
      361 gagtcacccc aatctgcacc ccgtctgggc caaggtcctc ggccttcaag ccgagcccgc
      421 ccgcgccacc cacgacacca gctcggcatc gccgctggcg gccgccatcg ccgcgatgac
      481 tgaccggcgc ggtgactccg agggctggca agccacccat cgcccgcagc gccccggcgc
      541 gccggtcgcc atgaagaagc gactcggccc gggcctttgg atcagcaccg tcgtgaaacc
      601 cggcgacgcc ggctacccca cccattcctg accgcaccct ccggagacac cgacatgaga
      661 ctgaacccgt tcagcaagaa gaccaccacc ggctactacg accgcctgaa ggccgagtgc
      721 gccgacctgg ccctgcaagt ccaggaggcc aaggccgccg ccgaagacgc gaaagcggac
      781 tacgaggcca aggtgcaggc cagccgcgac atggaagtcc gctacgccga caagcacttc
      841 ctgaccgagc aggagagcca ggcgcggcgg gagcgcgacg acgcctacaa ccgggccgag
      901 gaactgcgcc gcaaagcctg ggagctggag cacaagcaca acagcctgcg ccaggccgtc
      961 gaggccccgg cccggctgga agcggctaag caaaccatcg agcacctgag caaggagcgc
     1021 gcggcactgc gcgatgaggt ggtgaagacc gaagccctga tcgccaagct gcggcgtcgg
     1081 gtcgaggacc tggagcagcg catcgcacgc gagacccagg cggccagccg gtcgatggtg
     1141 gatgcggacg gcgagttcgt gatgcccgag gccttgaccc gcctggacgt ggagctgcgc
     1201 gtcacgcgcg ccacgctggc cgagcggcag accaagctgg ccgagttgaa ggacgcgctg
     1261 gagctgatcc cggaccaggt gcgcgaagcg cagcgaacct atggcagtgc gcgcgcggtg
     1321 gtcgcggaga tcgagctgca ggagcagttg ccgaactggc tggaggtcat tgccctggcg
     1381 gccctgtcgg cgtccagcga tgcgcgcaag tacgtcatcg agatcccgcg tgaggtggcc
     1441 gaggcggccc gggcgaagct ggctgcggac atgccgaagg cctgacacct gaagtggaag
     1501 ggtgggcggc gggcagccgg ggcatatccg ccgttcgcct cggtctggcc tccgatcgtt
     1561 cagccagatc gctcctgccc atgccccgta cggcccaccg gcgcccagcg cgaacggcat
     1621 cgcgccaagc cggccccgaa cccaggagaa ccgaatgcaa gttgaagatg ttgaaggcgg
     1681 gtgcaacacc gccgcgctgt tgcccatcgg ccgcgacacc tcggtcgtcg aactgatggt
     1741 gatgggctgg ttcggtgccg gccacgaggt gctgtggatt ccgatcattg aagtcgggcc
     1801 cggcagggat ggcgccgagc aggcccgcgt ggacgccgag cagatcggcg aaccccgcgt
     1861 gttgcgcgcg ctggccgatc tgggaggtct gccccagggc atcaagggct ggctgttccc
     1921 cgctcaccgg gacgacacct gcatcgtggc ctgccgcgag gacggcgcga cggtgacctt
     1981 cggatcgctg acccacccga tcaccgggcg cacgctgacc ttccgcaccg acacggaaag
     2041 cggcctgctg gatctgcgcc gtctcaacgc cgcgacagca gcgtcggggc agagcgcggt
     2101 ttgacttggc ttctgcttgc cacagcgcgt tcatgcaact ccctcaaccc tcggagtccg
     2161 accatgaccc aaccctccct ggcggcgcgc ctcgcggcac tgccaaccct gccgatgccg
     2221 cagctgtggc ggctgtggga tgaacacttc ccgcgccgcc cgcagcgcgt caaccgccgc
     2281 tacctcgaag cacgcctggc ctatcgcgtg caggaggcgg cactgggcgg cctgccgcgt
     2341 gtgatcaagg agcatctggc cgactgcggc gagcggcact ccaagctcaa gaccggccgc
     2401 ggccccgaac tgcggttgat gcccggcacg gtgctcgtcc gcgagtggga tgcccgtgaa
     2461 taccgcgtca aggtgacgga cgaccgacgc tacgaactcg atggccagtc gtacaagagc
     2521 ctgtcggcgg tggcgcgggc gatcaccggt gcgcactggt cgggcccggc cttcttcggc
     2581 ttgaagggga cgcgcaaatg aacccggtgg caccgaagcg ctgcgcggtc tacgcccgcg
     2641 tctccagcga cgagcggctg gcccagtcct tcaactccat cgacgcacag cgcgaggcgg
     2701 gtgccgcctt cgtcgccagc cagcgggccg aaggttggat cgtcacgggc gacgactacc
     2761 tcgaccctgg ctactccggc ggcaacatgg accgaccggg gctcaagcgc ctgatggccg
     2821 acatcgaagc cggccgggtc gacaccgtga tcgtctacaa gatcgaccga ctgacacgca
     2881 gcctggccga cttcgcccgc ctggttgaag tgttcgaccg caacggcgtg tcctttgtct
     2941 cggtcacgca gcagttcaac acgacgacgt cgatggggcg gctcacgctc aacatcctgc
     3001 tgtccttcgc ccagttcgag cgcgaggtca ccggcgagcg catccgcgac aagatcgcgg
     3061 ccagcaaggc caagggcatg tggatgggcg gcgtcgtgcc cctgggctac cgggtcgacg
     3121 cgcggcgact gctggtcgag cctgtcgagg ccgagaacgt gcggcggatc ttcacgcgct
     3181 ttgccgaatg ccgctcgacc accgatctgg tgcgatggct ggccgcccag ggcatcgcct
     3241 cgcgccgtgg cacgccgttt tcgaagcagg cgctgtggaa gctgctgaac aaccgcatct
     3301 acctcggcga gatcgtgcac aagggccagg tgttccgcgg ccagcacgag gccatcatcg
     3361 acaccagcct gtgggacgcg gtgcaggcgg tgttcgcggt caatcacaac gagcgccgcc
     3421 acgccacctg gcgcaaacgc ggcccggact tcctgctaac cggtctggtc gtcaccgagc
     3481 agggcgagcg cctgcaaccg tcgttcacga ccaagggcag gacggggcag cactaccgct
     3541 actacgtgcc gaagtcggtg caggccttcg gcaccaagcg cgccggtcac gccgtcggcc
     3601 gcctggccgc cgagccgctg gaagaattgg tgctggcgca gatccacgcc gccctgcaaa
     3661 cgcccgagcg gattcagggt gcgtgggacg cggtgcgggc gcagggggcc gcgctcaccg
     3721 agcctgaagt ggtgctgccg atgctgcaca acttcgcggg cgtctggcag gcgctgtatc
     3781 cggccgagca gcagcgcatc gcccggctcc tgatcgagtc cgtggtggtc agcgagcagg
     3841 cggtgtctat cgtctggcgc gacgcgggtt gggctgagct ggctcgggag atgcgcccgg
     3901 acggcattgg ggcggagttg ctggaactgg aagccgaagg agccgccgta tgaccaaggt
     3961 cctgcagacc ggggccgcgc aggcgggccc gaagctcacc accgtgatcc cggtgcgcat
     4021 caagcgcagc ggcgggcgcc ggtggatgat cccggccgcc ggtggggcgg acggcggcgc
     4081 cgaggcgccg aagcacgaga tgcccgtgct caccgctttg tcgcgcgcct ttcactggca
     4141 gcgtctgctc gacgaagggc gggtggccag cggcagcgag atcgctcggc gcgagggcct
     4201 gcatccgacg acggtcaacg aactgctgcg actgaccttg ctgtcgcctg ccgtggtgcg
     4261 ggcgctgctg gccggcaagc agccgcgtac tctgagcctg atctggctga agaacaacga
     4321 ggtgcccagg tcctgggacg atcaggagcg gttgttcggt cgcatggacg ggttcgagtc
     4381 tgcgccggtc gccggcgttg accgcgaacc accgcgcgca agtcgttgat ttatcgaccg
     4441 accgtgcatc ggccagccac aggtcgcatg gagaaaagag aggctgggcg ggtcggaagg
     4501 gcggggagag tcacccgctg accagcggcc aggcaccgca tcaacccgcg aacccgcgca
     4561 agtgctggcg atccgggcaa gaaaaaagcc aggcgagacg cctggcttcc aaaacggtgg
     4621 tggagcggag gaggatcgaa ctcccgacct tcgcattgcg aacgcgacgc tctcccagct
     4681 gagctaccgc cccgaagacg aaaaattata cacgccttta cctggcctcg gcgccgccgg
     4741 ccgccgcccc gtcggtttcg ggcacgtaga ccatcgtccc gttcttcagc cgataagccg
     4801 tcccggcctt cgcgccctcc ccctcgccga tctggcccga agaccggtac tccttcaact
     4861 cgttgccctg cctgatcagc atcgccatgt cgggcttgcc ggggttacgg atgaccaccg
     4921 cgtcctgcgt ggcgaacggg ttcagcgggt tctcgatcac cgcgatcagc agcaccgcga
     4981 tgatcaccag gaacacgtcg acgatgttca ccacgctgag gatcggatcg tcgccgtcgt
     5041 cgtccgacag aatgtcgatg cccatgcggc gctcacgcat atgccgctcc ttgcgtctcg
     5101 cctttggccg ccgcaaagga gtccgcacgc ccctgcaaca ggccctcgag ctcctccagc
     5161 agccagcgac ggcgcaccga atgcacgacg aaggtcatcg ccgccgacag cagcgcgacg
     5221 atcaccgcgg caaaggcgac caccaggttc tgcgccatgc cgtgggtgtt gccgttggcg
     5281 acggcgatca gcgccggccc catcgggatc atcgttgcca ccagcccgag catcggcgcg
     5341 atgcggctga cgatgcgctg catctccagt tgcctgagca cctgcaattc cagcgcctcg
     5401 gggctcgcgc ccggccggcg gcgcagccat tgcgcgagcg gtcgccgccc ctgcccacgc
     5461 gcggcgcgca gcgccgactc gaaaatcagc atgcccaggc agaacagcgc gtagacgaac
     5521 atcagcgaca acagcaccag cactggcgtg aggaagaacc gggagacctc gtagaggaag
     5581 ttctcgatcg cgttcatggt gtttccggtt ggggttcgat cccgaatgag gaagacgggg
     5641 gaaggtggtc aggccggcag cgtggtgcgg cgccgatcgc gccaggtgac ccacgcctgg
     5701 aagccggcgc cgccgacgat gaccagcccg atcatccagg cgtagatcca gaccagccgc
     5761 tcgagacgcg cctcgttgcg gacttcgcgc agctcttgcc cgcgaactat ctcgggcggg
     5821 cgcggcgcag gaggcggcgg cgccgcctcc gcgggccgag gttcaggcga cgcgcgcggc
     5881 cgcggggcgg gcggggccga ttgcgcgggt acccgcggct tcacgccttc cacgccgaag
     5941 ccgcgcgccg cctcgaccac gtaggcccgg aaagcctcgt tgggcgtgta gacgtcgtgc
     6001 ttcttcgcga gttcggcgta gacccgaacc agctcgcgcc gcgtgcgctc gtcggccttc
     6061 cagtagtcct tgcggatcgc ctcgatcatg cgctcggcga tctgcgccag cgcagtcggg
     6121 ttcgcgttct cgaaccactt gcgcatgccc agccggtagc ggtccttcag gtagacctcg
     6181 tggaattcct gccactgatc gtcgcgcacc acgttgcgat ccatgacctg ccagccccag
     6241 aagttgttca ccgcgttgag catctgcagc gtgccggcat agccttcctt ctgcatttcg
     6301 gcgacccagt tcgggtgctg gtagaccgag cgcagctcgg tggccatgaa cctcgaggcg
     6361 ctctccagct tcgcgcgccg aggatcgcgc atgttcgaga tgtagagctg cgggttgcgg
     6421 ccgtcgaggt gcttcaccgc catcgagatg ccgcccaggt actcgaacgg gtggtcggtg
     6481 tcgagcaggc cgcgcaggtt cgacgagcgc gagaacaccg ccgcgctggt gccgcgcaaa
     6541 tgctcggcgt aggcgttgat ctcggcgccg ccttcgtcgt gcagcttgcg gctccagttg
     6601 gccggatcgg gcccgtaggc ccaggacatc cgcgacaggt agagctgctc gagcctgccg
     6661 tcgccggctt cccacttgtc ggaggcgagc gtcgcgtcgg tgagccgcgt gctgtagtcg
     6721 ccgctctcgt tgccgaagac gcgcgtgagc gcgaactcgc gcgcctgctc cggcttcacg
     6781 ccacgttgcg acagctgcgc ccggatgcgc tcggtattgg cgcggacgaa gttctgctgc
     6841 gcaggctcgt cgagcgccga cagcatcacc accgcctcgt tgaagcgctc gatcacgttc
     6901 gggaactggt ctcggtacag gccggtgagc gagatcaccg cgtcgatgcg cgggcggccc
     6961 agctcgacct gcgggatcgg ttcgacgccg gtgacgcgcc cgccctcgtc ccagaccggc
     7021 ctcgccccca tcgcgtacag cacctgcgcc tccagcatgc cgaggtgccg catggattcg
     7081 gtgctccaca tgctgaacgc gagcttctcc gggaagcgcc cctgctgctc gcgatggcgc
     7141 tcgatcagct gctgcaccgc ctgccggccc gcttcccagg ccgcgcgggt cggaatgcgc
     7201 gacggatcga agccgtacat gttgcggccg gtcggcagcg cgtcgggatt gcggatcggg
     7261 tcgccgccgt acgaggggtc gatccagcgc gccgccagtc cgcgcagcac gccgtcgatc
     7321 tcccggtcgg cgcgcagtgc gtcgcggaac tggcggccgc gctgcgcgag gcgccgcagc
     7381 tccaagtcgg cgatcccgtc gagcggcccg tccgcgaaca ccacctcgtc gacgaagcgg
     7441 tacggcgcgc tttgctgcag ttgccggtag tcgccggcga atgcggcgcg ggcgctgccg
     7501 gcgccggtgc gctcgtacag cggctggccc agcatctgca tcacgttgct caccaggtgc
     7561 gcgcgctcgg cgtcgcgccc cagcgtgtgc agcccgagcg gctgcatcga cgcgcccagt
     7621 tcttcgagat agtcctcgat gtcgcgcagg aagccctcga agtcgcgctc caggtcggcc
     7681 accgtccagc ccatgtcgcg gtggatgttc atcttcaccg cctgcgcgat gatcagcccg
     7741 cggttattgt ccttcaccag gccctggtcc agcgaagcgt attcgcgaat gaggtcgccg
     7801 atgcgcacga agtcgctcga caggccggcc ggagcgaagg ccggcgtctg gtggctgacg
     7861 atcacgccgc ggccgcgccg cttcacgtgc accgcctcgc cgatgttgtc cacgatgtac
     7921 gggtacacca ccggcgtgtt gccgacc
//
LOCUS       DAORUQ010000149         7903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10249_length_7903_cov_5.400102, whole genome shotgun sequence.
ACCESSION   DAORUQ010000149 DAORUQ010000000
VERSION     DAORUQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_7903_cov_5.400102"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLW13_12125"
     CDS             <1..270
                     /locus_tag="PLW13_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71170.1"
                     /translation="LHDGLALAETPDCALAELHGAQSWLLLATAELAVPAGAVLEMDV
                     GWPWSADFATAAAAMARAEAPAAAPTPPRGWLARLTRHVFRTPPP"
     gene            complement(369..>2465)
                     /locus_tag="PLW13_12130"
     CDS             complement(369..>2465)
                     /locus_tag="PLW13_12130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY71171.1"
                     /translation="LGRRLRRVRYLVRRVPLALHVLAEDGPAALAARARAKLRAPRFV
                     ATAPPPAGLEAIGPLRLGTCDDARPPAVSIVIPVYGHHDHTFNCLHSLARFTALERVE
                     VIVVDDASPEPAAQALAGVEGVRWLRAAVNGGFIASCNLGAAAARGEFLVLLNNDIQV
                     TAGWLEALLAVFDSRPDAGLVGARLVYPDGSLQEAGGIVWRDGSAWNWGRGDDPERPP
                     YRYLRAVDYCSGACLALRMSDWQALEGFDRAYAPAYYEDTDLAFRVRQLGKRVYYQPQ
                     ATIIHYEGISSGTEESQGVKRHQVINRETFLARWRGVLATHRPNAVEPALEADRGAVA
                     RVLVVEACMITPDHDSGSVRMQAMLELMVGLGCKVSFVADNLEYRQPYVRDLQQAGVE
                     VWHHPFVASVEQLLQERGGEYDLIVLCRHYIAINYIDAVRRWAPRARIVFDTVDLHYL
                     REQRLAELEQSAALAGAAATTRRQELGVIARADITLVVSPVEQQLLATEAPGADVRVL
                     SNIHELRPDPRPWAERQGLIFVGGFRHPPNVDAVNWFVAEVWPAVRARLPGVELSIVG
                     SNMPAAIRDLAVAGVRVLGYVEDIDPLLDAARVSIAPLRYGAGVKGKINQAMAFGLPV
                     VATAMAAEGMGLREGEELLVAESAQAFADAVVRLYGDEALWQRLAEGGRDNVRRHFSR
                     AHAREILADMLGLPLP"
     assembly_gap    2466..2565
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2566..3817)
                     /locus_tag="PLW13_12135"
     CDS             complement(<2566..3817)
                     /locus_tag="PLW13_12135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY71172.1"
                     /translation="MPSRPRLLLISHDLGGGVERHVRDLRALLGACADADLLYPHDAS
                     TVVMEAAGAGPAWWRFQDWDDVVAALAARGYDRLGIHHVHGFTSAILDLPSRLGLPYD
                     LTIHDFYPYCPIHSLSAPEGGYCGEPDAEGCARCVLGRPHPWGLEIGEWRARMYRFLA
                     GAARVIVPSRFVAGRLARHFPDVRYDVRPHPPRQEWLEPARERVKVGLLGGLAAVKGL
                     ATVLASARLARDTGQPLAYCVLGYPESAIPTWPELPVQVRGEYRDADLPELLALERCD
                     VLWFPGRIPETYSYTLDVALASGLPIVASDLGAVAERLRDAGRGTLLPADAPAERWNE
                     ALLAAGASAREALAVIRGAEARREYQDWFLAPVTMAVRDVVPPVAPPGSLEVLGQPPE
                     SASPPLSLETLYEHGVECGHRESRL"
     gene            complement(3835..4695)
                     /locus_tag="PLW13_12140"
     CDS             complement(3835..4695)
                     /locus_tag="PLW13_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY71173.1"
                     /translation="MNTALSQWRQTLRSHAAFAAWHAADGAVLDDQWRPTIALQAALR
                     DAKASASVPGYCWPCQAPSQFLYDLKYSNGRDVNWRERLVCTRCGLNNRLRLTVQVIE
                     AEGLRGRGYLTEHVTPLAHLLLERIPGLVTSEYLGPDHAPGQVSRKGIRHEDLCRLSL
                     ADASVDFVVSCDVLEHIADYPAALRELARVLVPGGLALITVPFLPHAADNLVRARLEG
                     GRVVHLLEPEYHGDPVGAQEGVLCYYHFGWQLLEDLRRAGFADAWLDLYWSADYANLG
                     GPQVFVMARR"
     gene            complement(4824..5702)
                     /locus_tag="PLW13_12145"
     CDS             complement(4824..5702)
                     /locus_tag="PLW13_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY71174.1"
                     /translation="MDRLEAMRLFTRVAELGSFAAAAQQLGVARSVVTRQVAALEAHL
                     GVKLMARSTRRLTLTSAGTAYLEKCRVILGLVDAAETDIAAERVAPRGPIRASLPLSF
                     GFARLAPLLLDFAARYPEIGLDMDFSDRRVNLIEEGMDLSIRITRRLEPGDVARRIGS
                     GRLLVAAAPDYLARHGRPRQPADLAHHECLGYTAAGSQRWSFMVDGQTEHVPVRSRIS
                     ANNGEVLAEAAAQGMGIACQPDFILARFLDAGRLQTVLEEFPVPELGIYAMLPGNRQI
                     PHRVRVLLDFLVEGLA"
     gene            5798..6610
                     /locus_tag="PLW13_12150"
     CDS             5798..6610
                     /locus_tag="PLW13_12150"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQY71175.1"
                     /translation="MTTTQPALFVPHGAPSFALRPGAAGAAMAAFAASLARPRAIVVA
                     SAHWDTAAPRVGSAGRLRTIHDFHGFPAPLYALDYPATGSPEAAAEVLEALRAAGIPG
                     EADAARGLDHGAWVPLRLMFPEADVPVIPLSIQGHDGPRAAWRLGRALAPLRERGFLT
                     IGSGNVTHNLRDYALARREGGRTPAYVRAFADWLAERLAARDIDALLDYRRLAPDAAR
                     AHPEEEHLLPLFVALGAGGDTAQATRLHAGIDDYVLAMDAWAFEPSTSGDRA"
     gene            6607..7152
                     /locus_tag="PLW13_12155"
     CDS             6607..7152
                     /locus_tag="PLW13_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQY71176.1"
                     /translation="MNHYTATAKTLHWLMALLLIGLFTLGVYMHELPLSPRKLQLYSW
                     HKWAGVTVFLLVALRLAWRLGHRPPALPGTMPRLLRRVASATHGLLYLLMFAIPLSGW
                     LMSSAKGFQTVYFGVLPLPDLLAKDAALGDALLQLHFSLNMLFAVLVIGHAGAALKHH
                     FIDRDDILARMLPRRTTEKKS"
     gene            7149..7724
                     /locus_tag="PLW13_12160"
     CDS             7149..7724
                     /locus_tag="PLW13_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQY71177.1"
                     /translation="MKRLALALAFGAALAPGANALEYTALRPEASAITFAYQQMGVGL
                     EGRFTRFDGQISFDPAQPEAASARIEVELASIDTGSPEGNDEVAGKAWFDTPSFPRAR
                     FVSQAVKALGDSRYEVSGTLAIKGREQAVVVPASVTTQGDSAVFEGRFTIKRGDFAVG
                     EGAWSAFDIVANEVHVTFRITAGSGTNPQGE"
BASE COUNT         1146 a         2860 c         2750 g         1047 t
ORIGIN      
        1 ctgcacgacg ggctggcgct ggccgaaacg ccggactgcg cgctcgcgga actgcacggc
       61 gcgcagtcgt ggctgctgct cgccacggcg gagctggcgg tcccggccgg cgcagtgctg
      121 gagatggacg tgggctggcc gtggtcggcg gacttcgcga ccgcggccgc cgcgatggcg
      181 cgcgccgagg cgccagccgc cgcgccgacg ccgccgcgcg gctggctggc gcggctgacg
      241 cggcacgtct tccgcacccc tcccccgtag gagcgggcca tgcccgcgac aatgtgcgat
      301 taccgaacgc acaatcgcgg gcgtggcccg ctcctacggg gacatctggt cgcgggcatg
      361 gcccgctcct acgggagggg gaggcccagc atgtcggcca ggatctcgcg ggcatgcgcg
      421 cgcgagaagt ggcggcgcac gttgtcgcgt ccgccctcgg ctagccgctg ccacagggcc
      481 tcgtcgccgt agagacgcac gacggcatcg gcgaaggcct gggccgattc ggcgaccagc
      541 agctcctcgc cctcgcgcag gcccatgccc tctgccgcca tcgccgtcgc gaccacgggc
      601 aggccgaagg ccatggcctg gttgatcttg cccttgacgc cggcgccgta gcgcagcggc
      661 gcgatcgaga cgcgcgccgc gtcgagcagc ggatcgatgt cctcgacata gcccagcacc
      721 cgcacgcccg cgacggctag gtcgcggatg gccgccggca tgttgctgcc aacgatgctg
      781 agctccacgc cgggcagccg cgcgcgcacc gcgggccaga cctcggcgac gaaccagttg
      841 accgcatcga cgttgggcgg atggcggaag ccgccgacga agatcagccc ctggcgctcg
      901 gcccagggac gcgggtcggg gcgcagctcg tggatgttcg agagcacgcg cacgtccgcg
      961 ccgggcgcct cggtggccag aagttgctgt tccacggggc tcaccaccag cgtgatgtcg
     1021 gccctggcga tgacccccag ctcctggcgg cgcgtggtgg cggccgcccc cgccagcgcg
     1081 gccgattgct ccagctcggc caggcgctgc tcgcgcaggt agtgcaggtc gaccgtgtcg
     1141 aagacgatgc gcgcccgcgg cgcccagcgg cgcaccgcgt cgatatagtt gatggcgatg
     1201 tagtggcggc agagcacgat caggtcgtac tcgccgccgc gctcctgcag cagctgctcc
     1261 accgatgcca cgaaggggtg gtgccagacc tccactcccg cctgctgcag gtcgcgcacg
     1321 tagggctggc ggtattcgag gttgtcggcc acgaagctca ccttgcagcc cagcccgacc
     1381 atcagttcca gcatggcctg catgcgcacc gagccggagt cgtggtcggg cgtgatcatg
     1441 caggcctcca ccaccagcac gcgcgcaacg gccccgcgat cggcctccag cgccggctcg
     1501 accgcgttgg gacggtgggt ggcgagcacg ccgcgccaac gcgccaggaa ggtctcgcga
     1561 ttgatcacct ggtggcgctt gacgccctgg gactcctcgg tgccggagct gatgccctcg
     1621 taatggatga tggtcgcctg cggctggtaa tagacgcgct tgcccagctg ccgcacgcgg
     1681 aacgcgaggt cggtgtcctc gtagtaggcc ggcgcgtagg cgcgatcgaa gccctccagg
     1741 gcctgccagt cgctcatgcg cagcgccaga caggcgccgg agcagtaatc gaccgctctc
     1801 agatagcggt agggcggcct ttcgggatcg tcgccgcgcc cccagttcca cgccgagccg
     1861 tcgcgccaca cgatgccacc agcctcctgc aggctgccgt cgggatacac cagccgcgcc
     1921 ccgaccaggc cggcgtcggg acggctgtcg aacacggcca gcagcgcctc gagccagccg
     1981 gccgtgacct ggatgtcgtt gttgagcagc accaggaatt cgccccgcgc cgccgcggcg
     2041 ccgaggttgc agctggcgat gaaaccgccg ttcacggcgg cgcgcagcca gcgcacgccc
     2101 tcgaccccgg cgagcgcctg cgcggccggc tccggggagg cgtcgtccac cacgatgacc
     2161 tcgacgcgct ccagcgcggt gaagcgggcc aggctgtgca ggcagttgaa agtgtggtcg
     2221 tggtgcccgt agaccgggat cacgatcgag accgcgggcg gcctcgcgtc gtcgcaggtc
     2281 ccgaggcgca gcggcccgat cgcctccagc ccggccggcg gcggggccgt cgcgacgaag
     2341 cgcggtgcgc gcagcttcgc ccgcgcgcgc gccgcgaggg ccgccggccc gtcctcggcg
     2401 aggacgtgca gtgccagcgg cacgcgccgc acgaggtagc ggacgcgccg cagccgccgg
     2461 ccgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccagg cgcgattcgc
     2581 ggtgaccgca ttcgaccccg tgctcgtaga gcgtctcgag cgataacggg gggcttgccg
     2641 actccggtgg ttgcccgagc acttccaggc tgccgggggg agccacgggt ggcacaacgt
     2701 cgcgcaccgc catcgtcacc ggggccaaga accagtcctg gtactcgcgc cgggcttcgg
     2761 cgccgcggat caccgcgagt gcttcgcgag cgctcgcacc tgccgccagc agggcctcgt
     2821 tccagcgctc ggcgggcgcg tcggcgggaa gcagcgtgcc cctgcccgca tcgcgcaggc
     2881 gctccgccac ggcgcccaga tcgctggcga caatgggcaa ccccgacgcc agggccacat
     2941 cgagggtgta ggaatacgtc tccggtatgc ggcccgggaa ccacagcacg tcgcagcgct
     3001 cgagcgcgag cagctccggc aggtcggcat cgcggtactc gccgcggacc tggaccggca
     3061 actcgggcca ggtcgggatg gcgctctcgg ggtagccgag cacgcagtag gcgagcggct
     3121 gccccgtgtc gcgggccagg cgcgcgctgg cgagcacggt cgccagcccc ttgaccgcgg
     3181 cgaggccgcc gagcaggccc actttcacgc gctcgcgcgc cggctcgagc cattcctgcc
     3241 gcggcgggtg cggccgcacg tcgtaccgca cgtcgggaaa gtggcgagcc agccgcccgg
     3301 cgacgaagcg cgagggcacg atcacccgcg ccgccccggc caggaagcga tacatgcgcg
     3361 cgcgccactc cccgatttcc aggccccagg ggtgcgggcg cccgagcacg cagcgcgcgc
     3421 agccctcggc atcgggctcg ccgcagtagc caccctccgg cgccgacagc gagtggatcg
     3481 ggcaataggg gtagaagtcg tggatcgtca ggtcataggg caggcccagt cgcgagggca
     3541 gatccaggat ggcgctcgtg aagccgtgca cgtggtgaat gcctagccgg tcatagccgc
     3601 gcgcggcaag ggcggcgacc acgtcgtccc agtcctggaa gcgccaccag gccggcccgg
     3661 ccccggccgc ctccatcacc acggtcgagg catcgtgcgg atacagcaga tcggcgtcgg
     3721 cgcaggcgcc cagcagtgcg cgcaggtcgc ggacgtggcg ctctacgccc ccgcccaggt
     3781 cgtgggagat cagcagcagg cggggacgcg atggcatgcg cagcgcccgg cggttcagcg
     3841 gcgcgccatg acgaaaacct gggggccgcc caggttcgca tagtcggcgg accagtacag
     3901 gtcgagccag gcgtcggcga aacccgcccg gcgcaggtcc tcgagcagct gccagccgaa
     3961 gtggtaatag cagagcacgc cctcctgggc gccgacggga tcgccgtggt actcgggctc
     4021 cagcaggtgg accacccgcc cgccctccag ccgcgcgcgc acgagattgt ccgcggcgtg
     4081 cggcaggaac ggcacggtga tgagcgcgag tccgccgggg accagcacgc gcgccagttc
     4141 gcgcagggcc gcggggtagt cggcgatgtg ctcgagcacg tcacagctga ccacgaagtc
     4201 cacgctggcg tcggccaggc tgaggcggca caggtcctcg tggcggatgc ccttgcgcga
     4261 gacctgcccc ggcgcatggt ccggtcccag gtattcgctg gtgaccagcc ccgggatgcg
     4321 ctccagcagc aggtgcgcca gcggcgtcac gtgctcggtc aggtagcccc ggccgcgcag
     4381 gccctcggcc tcgatgacct gcacggtcag gcgcaggcgg ttgttcagcc cgcaacgcgt
     4441 gcacaccagg cgctcgcgcc agttgacgtc acgcccgttc gaatacttga ggtcataaag
     4501 gaactgcgag ggcgcctggc atggccagca gtaaccgggc accgaggcgc tggccttcgc
     4561 atcgcgcaac gcggcctgca gcgcgatggt cgggcgccac tggtcgtcga gcaccgcgcc
     4621 gtcggcggca tgccatgcgg cgaacgccgc gtggctgcgc aacgtctgcc gccattggga
     4681 aagtgccgtg ttcatccctg aggtgtccaa tcagtgccgc tgccgggggc gattgccggc
     4741 gacggcgcaa aagcgcgcag cataaggccc gtctcgaccc ggaaccagcc cccgggcgcg
     4801 gcggcacatc cgcgcctgcg gcctcaggcg aggccctcga ccaggaaatc gagcagcacc
     4861 cgcacccggt gcgggatctg gcggttgccg ggcagcatgg cgtagatgcc gagctccggc
     4921 accggaaact cctcgagcac cgtctgcagg cgtccggcgt ccaggaaacg cgcgaggatg
     4981 aaatcgggct ggcaggcgat gcccatgccc tgcgccgcgg cctcggccag cacctcgccg
     5041 ttgttggcgc tgatgcggct gcgcaccggc acgtgctcgg tctgcccgtc caccatgaag
     5101 ctccagcgct ggctgccggc cgcggtgtaa ccgaggcact cgtggtgcgc caggtccgct
     5161 ggctggcgcg gccggccgtg acgcgccagg taatcgggcg cggcggcgac cagcaggcgt
     5221 ccgctgccga tgcgccgcgc cacgtcgccg ggctcgagcc ggcgcgtgat gcggatcgag
     5281 aggtccatgc cctcctcgat caggttcacg cgccggtcgc tgaagtccat gtcgaggccg
     5341 atctccggat agcgcgccgc aaaatccagc agcaagggcg ccagccgcgc gaagccgaag
     5401 ctcaggggca ggctggcgcg gatgggtccg cgcggcgcca cccgttcggc cgcgatgtcg
     5461 gtttccgccg catcgaccag gccgaggatc acgcgacact tctcgagata agccgtgccc
     5521 gccgaggtca gcgtcagccg gcgcgtgctg cgcgccatga gcttcacgcc gaggtgggcc
     5581 tccagcgccg cgacctggcg cgtgaccacc gagcgcgcca cgccgagctg ttgcgccgcg
     5641 gcggcaaagc tgccgagctc cgccacccgg gtgaacaggc gcatcgcctc gagccgatcc
     5701 attgttgcgt ttccagcaat aaagatttgc cgattgtctg ctatttgagc atccaggggg
     5761 agcgtacagt tcacccatcg tttggaggcc cacacccatg accacaaccc agcccgccct
     5821 cttcgttccg cacggcgcac cgagcttcgc gttgcgcccg ggcgcggccg gggcggcgat
     5881 ggcagccttc gccgcctcgc tcgccaggcc acgggcgatc gtcgtcgcca gcgcgcactg
     5941 ggacaccgcg gcaccgcgcg tcggcagcgc cgggcggctg cgcacgatcc acgatttcca
     6001 cggttttccc gccccgctct acgcgctcga ctacccggcc accggcagcc ccgaggcggc
     6061 cgccgaggtg ctcgaggcac tgcgcgcggc aggcatcccg ggcgaggccg atgccgcgcg
     6121 cggcctcgac cacggcgcct gggtgccgct gcgactgatg ttccccgagg ccgacgtgcc
     6181 ggtgataccg ttgtcgatcc agggccacga cggcccgcgc gcggcgtggc gcctgggccg
     6241 ggcgctggcg ccgctgcgcg agcgcggctt cctgacgatc ggctcgggca acgtcacgca
     6301 caacctgcgc gactacgcgc tggcgcgtcg cgagggcggg cgcacgcccg cttacgtgcg
     6361 cgccttcgcc gactggctgg ccgaacggct cgccgcgcgc gatatcgacg ccctgctcga
     6421 ctaccggcgc ctggcgccgg acgccgcgcg cgcacacccg gaggaggaac acctgctgcc
     6481 gctgttcgtc gcgctcggcg ccggcggcga caccgcacag gcgacccgcc tgcatgccgg
     6541 catcgacgat tatgtgctcg cgatggacgc ctgggccttc gagccctcca ccagcggaga
     6601 ccgcgcatga accactacac cgcgaccgcg aaaaccctgc actggctgat ggcgctgctg
     6661 ctgatcgggc tgttcacgct cggcgtctac atgcacgagc tgccgctgtc gccgcgcaag
     6721 ctgcaactct attcgtggca caagtgggcc ggcgtcacgg tcttcctgct ggtggcgctg
     6781 cggctcgcct ggcgtctcgg gcaccgcccg ccggcgctgc ccgggaccat gccgcggctc
     6841 ctgcgccgcg tggcgtcggc gacgcacggg ctgctctacc tgctgatgtt cgcgatcccg
     6901 ctgtcgggct ggctgatgag ctcggccaag ggcttccaga ccgtgtactt cggcgtgctg
     6961 ccgctgcccg acctgctggc aaaagacgcc gcgctgggcg acgcgctgct gcagctgcac
     7021 ttcagcctga acatgctgtt cgccgtgctg gtcatcggcc acgccggcgc cgcgctgaaa
     7081 caccatttca tcgaccggga cgacatcctc gcgcggatgc tgccccgccg caccacggag
     7141 aagaaatcat gaaacgcctc gcactcgcgc tcgccttcgg cgccgcactc gccccgggcg
     7201 caaacgcgct cgagtacacc gcgcttcgcc ccgaggcgag cgcgatcacc ttcgcgtacc
     7261 agcagatggg cgtggggctc gaaggccgct tcacccgctt cgacgggcag atcagcttcg
     7321 accccgcaca gcccgaggcg gccagcgcgc gcatcgaggt ggagctggcc agcatcgaca
     7381 ccggctcgcc cgagggcaac gacgaggtgg ccggcaaggc ctggttcgac acgccgtcgt
     7441 tcccgcgggc acgcttcgtc tcgcaggccg tgaaggcgct cggcgattcg cgctacgagg
     7501 tgagcggcac gctggccatc aagggccgcg agcaggccgt cgtggtgccc gcgagcgtca
     7561 ccacgcaggg cgacagcgcg gtcttcgagg gccgcttcac gatcaaacgc ggcgatttcg
     7621 cggtcggcga gggcgcctgg tcggctttcg acatcgtcgc caacgaagtt cacgtcacgt
     7681 ttcgtatcac cgccggttcc ggcaccaacc cccagggaga atgaccatga agaaactcgc
     7741 cacgctttcc gccgccctcg tgctcgcggg cgccgctgcc gcgccggcat tcgcggcgcc
     7801 gcagacctac gtgatcgacg gcacccacac gctgccgcgc ttctcttaca gccacttcgg
     7861 cttctcgacc cagctcagcc ggttcaacaa gaccaccggc acg
//
LOCUS       DAORUQ010000150         7813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10425_length_7813_cov_4.870972, whole genome shotgun sequence.
ACCESSION   DAORUQ010000150 DAORUQ010000000
VERSION     DAORUQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_7813_cov_4.870972"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..2142)
                     /locus_tag="PLW13_12165"
     CDS             complement(217..2142)
                     /locus_tag="PLW13_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71178.1"
                     /translation="MKNKNQHGKKHLACLALGLTHAMALLAVDAAPELKSTLVLPEIV
                     VVGKRMAQAPAITFHETTALDFAAWNATTAADALTRTPGVNVQYGAESGEARAWIRGF
                     RDRSVLVLFDGIPVASGFEGTIDLNEISIENVSRIKTMTAAPSVIYGTNGIGGVIDVV
                     PEALPEERRIASRVEVGDNDARLYSASYADRAESFGILAAASHAEADDFRLSGDHASE
                     VNQPGSRRVNSDYQRRSALLRLSNESQRLGESAVLLNLSDTERGTPPQAGVDDPDFQR
                     LTESKRRTVGLSHSFAAVPVAVKLFHNHYEYELITYTDANYDEIDEIEQAEDYAFGGK
                     AYATVGIGQRNTLVASLAAVEEVYEAQEVFPGADEASLRTYNAALEDEWWIRDNVSVG
                     LGAIYTYFDQIEIGESSSAINPQVVASWQLSNGLSLRASAAQRSRFPKLRELYRLRYG
                     NPGLQEEVANSYEIGLRHQLDNGLGLDMAVFTIDLDGMIDRPTRNSPYQNLDDSEIKG
                     FELSASGWVSEQVYLLAGYSLVDAAEELPDGSARQLRSRPEHTVTGELRYRFPNRLLL
                     SLNAIYVDGLHDLDGDDVYTKISSYVVAHMKASLPLTEVLQCYASVGNLADADYEQKL
                     GYPREGRTLRVGLELEL"
     gene            complement(2180..3067)
                     /locus_tag="PLW13_12170"
     CDS             complement(2180..3067)
                     /locus_tag="PLW13_12170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY71179.1"
                     /translation="MPLSPESILPDEPLRESAPVAYRLAHRHCPTGPDGCRDYHAMWQ
                     YFRQIGLLTTIGTNTDFLVATLRRLADADSCRRVLISATADYGMLAHVLHAYRLAGVV
                     PDVTVVDMCETPLALNRWYAERAGVTIRTCRANLLEFRADAPFDLVCTHSFLGRLTQE
                     VRAAVLDKWHEVLRPGGHVVTVARVRYGAPDGGVTRYEPAEIVEFRDRALALARAKQA
                     EIDLPAEAIADAAEHYAVSKRSRGFGSTGMLRALFEDHGFALERFDSGGEEERRRDRP
                     SCPTLGWKSERFRIVAVRR"
     gene            3200..5698
                     /locus_tag="PLW13_12175"
     CDS             3200..5698
                     /locus_tag="PLW13_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQY71180.1"
                     /translation="MQIESVKMLPGRHRAGDCTALELVLSCAPETAAALRRQADALGA
                     WARSQLAGALHATPALDAEGEPAPSAGNPLEDVARCHAGLALWLQRAGGHEARWFALS
                     GPEGAHGFRYCLEYDHHEVAASACALALRLMAGFLRAQGIEPDEYRAAGAPPAEPLPQ
                     AVAAFLELARATALPLDTTVLIDIARERDIPWMKGDRPPFASLSTQRIRRNGALRLGF
                     GDRQHVLDGTLCLDRAGPWPGLLDGIDAMYRLLEEVGAQLPQRTACSSPLRARRNAHA
                     LGYPVAVRPQSRRAPEGAPQPARRHVDSDAGLEQALQALRALDGGVVIERDPAGTLHH
                     LVVANNRLAAVLAEGRPLDIEAVHPELLAVIARLCDRLALGVFALDVIATDIAAAPMA
                     AGTRVVDLSLAPELDRLIADRALHREVLEAYMQWLFPRGEDGRIPVVAITGTNGKTTT
                     TRMIDAVLRAAGHRTGMACTDGVFIAGQTVNSGDSAGSVFHYQVLQDLRISHAVLETA
                     RGAVADFGFAWDACDVAVCTNVTAEHLGQFGIETVEDMARLKQCVLRRARRGVVLNAD
                     DAHARSMSVGLQAPAICWSSTTQPVAQLAQAHRDAGGAGKAASFVVVEALDGADWIVL
                     YTAHNAMRTPVMAVDAIPATFAGRAAHNVSNALQAVAATQMLGAPIGATRQALGAFDT
                     AWENTPGRLNIIRESPFTAIIDYAHNPDSYRALVAFIDRWPVARRKILMIRCPGRSSR
                     EAVGQIAGLVAGHFDVFVCADAREPVNFAQGELSALIGQELLAHGVAADAVTVVPVEA
                     EAIAHALALATTGDLVVLCTTASMKENARQQILA"
     gene            complement(5720..7630)
                     /locus_tag="PLW13_12180"
     CDS             complement(5720..7630)
                     /locus_tag="PLW13_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HQY71181.1"
                     /translation="MIFADALAGLGAYVDDPAVLAALLVAVPLGVLAGVVPGLGGKLS
                     IVMAIPFLVGADPTVGAVFLLAMHSVIHTGGPIPSILLGVPGSGPDAATVVDGHPMAR
                     SGQAGRALGAALASSCVGGIFGALVLALALPLVKPVLLSFGPPEVFMLAVLGITFIAA
                     VSGDRLARGLMVGCIGLLVSFVGMDPIGGVARYSAGQLFLMDGVDLITAVVGLFAIPE
                     MLALMRQQAHHGALPRGGFEERGILEGMLDVWRHRYLTLRASLIGTVIGIIPGLGGDV
                     ASWVSYGHAVQSSSRPGEFGKGRVEGVIAPEAANNSKEGGSLLPTLFFGIPGSSGMAV
                     LLGAFVTLGITPGPAMAGQGLALVWTLIWALVLANVLAVAVFLPSAGLLARVAGVSGA
                     AMFPFVLCFALIGTYLSALHWQTFFVLLGFGLLGVALKHHEWPRGPFAIGLVLGTPAE
                     IALHQSLTIWGGSFLLRPLTLVLIALVLASLWQSLRRYRRSGDVHAPDPGLLGVCAGV
                     FGLALVTALRYPAPSSVLPALIAGVGALLALACGVVMLRNRPLSLAPDGAVGWRSHLP
                     ALAWLALFVVAVLALGLSPGLPLALLVFHLAARRGGVMRALLAAVAMLVIVEGVFAGI
                     LGVSIYRGLVGG"
     gene            complement(7627..>7813)
                     /locus_tag="PLW13_12185"
     CDS             complement(7627..>7813)
                     /locus_tag="PLW13_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71182.1"
                     /translation="VAPPGVPPERLAVLREAYLDTLADPELLREAAAMRLPIEALDGA
                     AVQERISRLLAQAVPGP"
BASE COUNT         1264 a         2811 c         2666 g         1072 t
ORIGIN      
        1 tgccctcgtt gtccggagtc gattatctgt gcctggcgcc aacgatacac cgctgacgcc
       61 tggtgtccat gaccctggcg ttccggccgc acgcatgccg gcgttgagcg ggccgggaac
      121 cctggatcat cagcgatact agccccctga cgccgcgccg ccatcgttca aacggacgag
      181 acgcgcccgc acgcaccgcg ccggcccgcg ggtcgttcac agctctaact ccagccccac
      241 ccgcagcgtg cgcccttcgc gcggatagcc aagcttctgc tcgtagtccg cgtcggcgag
      301 attgcccacc gaggcatagc attgcagcac ttcggtgagc ggcagcgagg ccttcatgtg
      361 cgcgaccacg tagctggata tcttcgtgta cacgtcatcg ccgtcgagat cgtgcaggcc
      421 atcgacgtag atcgcgttca gcgacagcag cagccggttg gggaagcggt agcgcagctc
      481 gccggtcacc gtgtgctccg ggcggctgcg cagctgccgc gcgctgccat cgggcagttc
      541 ctcggcggca tcgacgaggc tgtagcccgc gagcaggtag acctgctcgc tcacccatcc
      601 gctcgccgag agctcgaagc ccttgatctc ggagtcgtcg aggttctggt acgggctgtt
      661 gcgcgtcggg cggtcgatca tgccgtcgag atcgatcgtg aacacggcca tgtcgagccc
      721 gaggccattg tcgagctggt ggcgcaggcc gatctcgtag ctgttggcga cctcctcctg
      781 caggccgggg ttgccgtagc gcagccggta gagctcgcgc agcttgggga agcggctgcg
      841 ctgcgccgcc gaggcgcgca acgacaggcc attggacagc tgccacgagg cgaccacctg
      901 cgggtttatt gcgctgctgc tctcgccgat ctcgatctgg tcgaaatacg tgtagatggc
      961 tccgagaccg acggagacgt tgtcgcgaat ccaccactcg tcctcgagcg ccgcgttgta
     1021 ggtcctgagg ctggcctcgt ccgcacccgg aaacacttcc tgcgcctcgt agacctcctc
     1081 gaccgcagcg aggctggcga caagcgtgtt gcgctggccg atgccgacgg tggcgtaggc
     1141 cttgccgccg aaggcgtaat cctcggcctg ctcgatctcg tcgatctcgt cgtaattcgc
     1201 gtcggtgtag gtgatcagtt cgtattcgta gtggttgtga aagagcttca ccgccaccgg
     1261 cacggcggcg aagctgtgcg acaaccccac ggtgcgccgc ttcgactcgg tgaggcgctg
     1321 gaagtcgggg tcgtccacac cggcctgggg cggcgtgccg cgctcggtgt ccgacaggtt
     1381 cagcagcacg gccgactccc cgagccgctg cgattcgttg ctgaggcgca gcagcgcgct
     1441 gcgccgctgg tagtcgctgt tgacgcgccg gctgcccggc tggttcacct cggatgcgtg
     1501 gtcgccggag agccggaaat cgtccgcttc cgcgtgactg gcggcagcca gtatgccgaa
     1561 ggattccgcg cggtcggcgt agctggcgct gtagaggcgc gcgtcgttgt ctccgacctc
     1621 gacccggctc gcgatccggc gctcctcggg cagcgcctcg gggaccacgt cgatcacgcc
     1681 gccgatgccg ttggtgccgt agatgaccga cggcgccgcg gtcatggtct tgatgcgcga
     1741 gacgttctcg atcgagatct cgttgaggtc gatggtgccc tcgaagcccg atgccacggg
     1801 aatgccgtcg aagagcacca gcacgctgcg gtcgcggaaa ccgcgaatcc acgcgcgcgc
     1861 ctctcccgac tcggcgccgt actgcacgtt gacgccaggc gtccgggtca gggcatcggc
     1921 ggcggtggtg gcgttccagg ccgcgaagtc gagcgccgtg gtttcgtgaa aggtgatggc
     1981 gggcgcttgc gccatgcgct tgccgaccac cacgatctcg ggcagcacca gggtcgattt
     2041 cagttccggc gcggcatcga ccgcgagcag cgccattgcg tgcgtcaatc ccagcgcgag
     2101 gcaggcaaga tgctttttcc cgtgctggtt cttgtttttc atgacgggtc tcctgtacgg
     2161 attccgacgc gcgcccggtt cagcgacgca cggcgacgat gcggaagcgc tcggatttcc
     2221 agcccagcgt ggggcaggat ggacggtcac gccggcgttc ctcctcgcca ccgctgtcga
     2281 agcgctcgag cgcgaagccg tgatcctcga acagcgcgcg cagcatcccg gtcgatccga
     2341 aaccgcgaga acgcttgctg acggcgtaat gctccgccgc atccgcaatc gcttccgcgg
     2401 gcaggtcaat ctcggcctgc ttcgcacgcg ccagcgcgag cgcccgatca cgaaattcca
     2461 cgatctccgc gggttcgtag cgcgtgaccc ccccatcggg cgcgccatag cgcacccgcg
     2521 ccaccgtcac cacgtgcccg cccgggcgca gcacctcatg ccacttgtcc agcactgcgg
     2581 cacgcacctc ctgcgtcagc cggcccagga aggaatgcgt gcacaccagg tcgaagggcg
     2641 catccgcgcg gaactcgagc aggttcgcgc ggcaggtgcg gatagtcacc ccggcccgtt
     2701 cggcgtacca gcggttcagc gcgagcggcg tctcgcacat atcgaccacc gtgacatcgg
     2761 gcacgacgcc agcgaggcgg taggcgtgca gcacatgcgc cagcatgccg tagtccgcgg
     2821 tggccgagat cagcacgcgg cggcagctat cggcatcggc gagccggcgc agcgtggcga
     2881 cgagaaaatc ggtgttggtg ccgatcgtgg tgagcagccc gatctggcgg aagtactgcc
     2941 acatggcgtg gtagtcgcgg caaccgtcgg gcccggtggg gcaatgccgg tgagccagcc
     3001 ggtaggccac cggcgcgctc tcgcgcagcg gttcgtcggg caggatggat tcgggcgaca
     3061 gcggcatgaa gactcctcga tacgatgcgg cgcgacgatg actaggcagg ccggcacgaa
     3121 agctgcaatc ctaacaccgg cgctgcccgg ttcgcccccc gccggagcgc gccaacgact
     3181 gttccccagg aaatgcccga tgcagatcga gtccgtcaag atgctccccg gccgccaccg
     3241 cgcgggagac tgcaccgcgc tggaactggt gctctcctgc gcgcccgaaa ccgcggcagc
     3301 gctgcggcgc caagcggacg cgctcggcgc ctgggcccgc tcgcaacttg ccggggcact
     3361 gcacgcgacg cctgcactcg atgccgaggg cgagccggcg ccatcggcgg ggaacccgct
     3421 ggaagacgtt gcgcgctgtc acgccgggct cgcgctgtgg ctgcagcgcg cgggcgggca
     3481 cgaagcgcgc tggttcgcgc tgagcggccc cgagggcgcg cacggcttcc gctattgcct
     3541 cgagtacgac caccacgagg tcgccgcgtc cgcctgcgcg ctggcgctgc gcctgatggc
     3601 ggggttcctg cgcgcccagg gcatcgagcc cgacgagtac cgcgccgccg gcgcgccgcc
     3661 agcggaaccg ctgccacagg cggttgccgc cttcctcgaa ctggcgcgcg ccacggcatt
     3721 gccgctcgac accacggtgc tcatcgacat cgcccgcgaa cgcgacatcc cgtggatgaa
     3781 gggcgatcgc ccgccgttcg cctcgttgag cacgcagcgc atccgccgca acggcgcgct
     3841 gcggctcggt ttcggcgatc gccagcacgt gctcgacggc acgctgtgcc tggatcgcgc
     3901 ggggccctgg ccgggactgc tcgacgggat cgacgcaatg taccggctac tcgaggaagt
     3961 cggcgcgcag ctgccgcaac gcacggcctg cagctcgccg ctgcgcgcgc gccgcaacgc
     4021 ccacgcgctg ggctacccgg tggcggtacg gccgcaatca cggcgcgcgc ccgagggcgc
     4081 gccgcagccg gctcgccggc acgtcgattc cgacgccggc ctcgagcagg cgctgcaagc
     4141 gctgcgtgcc ctcgacgggg gagtcgtgat cgagcgcgat cccgccggca cgctccacca
     4201 cctggtggtc gcgaacaacc gcctcgcggc ggtgctggcc gaggggcggc cgctggacat
     4261 cgaggccgtg cacccggaac tgctcgcggt gatcgccagg ctgtgcgatc ggctcgcgct
     4321 cggcgtgttc gcgctcgacg tgatcgcgac ggacattgcc gccgccccga tggccgccgg
     4381 cacccgcgtc gtggacctct cgctcgcccc cgagctcgac cggctgatcg ccgaccgtgc
     4441 gctgcaccgt gaggtgctgg aggcgtacat gcagtggctg ttcccccgcg gggaggacgg
     4501 gcgcataccg gtcgtcgcga tcaccggcac caacggcaag accacgacga cgcggatgat
     4561 cgacgcggtg ctgcgcgcgg cgggccaccg caccggcatg gcctgcaccg acggcgtgtt
     4621 catcgccggg cagaccgtca acagcggcga cagcgcgggc tcggtgttcc attaccaggt
     4681 gctgcaggac ctgcgcatct cgcacgcggt gctggaaacg gcccggggcg cggtcgcgga
     4741 cttcggcttc gcctgggacg cctgcgacgt ggcggtgtgc accaacgtca ccgccgagca
     4801 cctcgggcag ttcggcatcg agacggtcga ggacatggca cggctgaagc agtgcgtgct
     4861 gcgccgggcg cggcgcggcg tggtgctcaa tgccgacgat gcgcatgcgc gcagcatgag
     4921 tgtcggcctg caggctcccg cgatctgctg gagctccacg acacagccgg tcgcgcagct
     4981 cgcgcaagcg caccgcgacg cgggcggcgc cggcaaggcg gcgagcttcg tggtcgtcga
     5041 ggcgctcgat ggcgctgact ggatcgtgct ctacacggcg cacaacgcca tgcgcacgcc
     5101 ggtgatggcg gtcgatgcga tccccgccac cttcgccggc cgcgccgccc acaacgtcag
     5161 caacgcgctg caggcggtcg ccgccacgca gatgctgggc gcgccgatcg gtgcgacccg
     5221 gcaggcgttg ggcgccttcg acacggcctg ggagaacacg ccggggcgac tcaacatcat
     5281 ccgtgaatcg ccgttcaccg cgatcatcga ctacgcgcac aatcccgaca gctaccgcgc
     5341 gctggtcgcc ttcatcgacc gctggccggt ggcgcggcgc aagatcctca tgatccgttg
     5401 cccggggcgc tccagccgcg aagcggtcgg gcagatcgcc gggctcgtcg cgggacattt
     5461 cgacgtcttc gtgtgcgccg acgcgcgcga gccggtcaac ttcgcgcaag gcgagctctc
     5521 tgcgctgatc gggcaggaac tgctcgcgca cggcgtcgca gcggacgcgg tgacggtggt
     5581 ccccgtggag gccgaggcaa tcgcgcatgc gctcgcgctg gccacaaccg gcgacctcgt
     5641 ggtgctgtgc accaccgcct cgatgaagga gaacgcgcgc cagcagatcc tcgcctgaga
     5701 gcccggggca acgcgcggct cagccgccga cgagcccgcg gtagatgctg acgccgagga
     5761 ttccggcaaa cacgccctcc acgatcacca gcatcgccac tgccgccagc agcgcgcgca
     5821 tcaccccgcc ccggcgcgcc gccaggtgaa agaccagcag cgccagcggc aggccgggcg
     5881 acaggcccaa tgcgagcacc gccacgacaa acagcgcaag ccaggcgagc gccggcaggt
     5941 gggagcgcca gccaacggcg ccatcgggcg cgagcgacag cgggcggttg cgcaacatga
     6001 ccacaccaca agccagcgcg agcaacgcac ccacgccggc aatcagcgcc ggcaggaccg
     6061 atgagggcgc cgggtagcgc agggctgtca ccagcgcgag cccgaacacg ccggcacaca
     6121 cacccagcag gcccggatcc ggcgcgtgca cgtcgcccga gcggcggtaa cggcgcaggc
     6181 tctgccacag gctggcgagc accagcgcga tcagcaccag cgtgagcggg cgcagcagga
     6241 acgagccccc ccagatcgtc agcgactggt gcagggcgat ttcggccggc gtgccgagca
     6301 ccagccctat cgcgaagggc ccgcgcggcc attcatggtg cttcagcgcc acaccgagca
     6361 gcccgaagcc caacagcacg aagaaagtct gccagtgcag cgcgctgagg taggtgccga
     6421 tcagcgcgaa gcagagcacg aaggggaaca tcgccgcgcc cgacacgccc gccacccgcg
     6481 ccagcagtcc cgcgctcggc aggaacacgg cgaccgccag cacattagcc agcaccagcg
     6541 cccagatcag cgtccacacc agcgccagcc cctgcccggc cattgccggc ccgggcgtga
     6601 tgcccagcgt gacgaaggcg cccagcagca cggccatgcc cgagctgccg gggatgccga
     6661 agaacagcgt agggagcagg ctgccgcctt ccttggagtt gttcgccgcc tccggggcga
     6721 tgacgccctc gacgcgcccc ttgccgaact ccccgggccg cgacgaactc tgcaccgcat
     6781 gcccgtagga cacccaggac gcgacatcgc cgccaaggcc ggggatgatg ccgatcacgg
     6841 tgccgatcaa cgaggcacgc agcgtgaggt aacggtgtcg ccagacatcc agcatgccct
     6901 cgaggatgcc gcgctcctcg aagccacccc ggggcagtgc gccgtgatgt gcctgctggc
     6961 gcatcagcgc gagcatttcc ggtatcgcga acagccccac cacggccgtg atcaggtcca
     7021 cgccgtccat caggaacagc tgtcccgcgc tgtagcgcgc cacgccgccg atgggatcca
     7081 tgcccacgaa ggacaccagc agaccgatac agccgaccat cagcccgcgc gccaggcggt
     7141 caccgctgac cgcggcgatg aaggtgatgc ccagcaccgc cagcatgaac acttccggcg
     7201 gaccgaagga cagcagcacc ggcttgacca gcggcagcgc cagcgccagc accagcgcgc
     7261 cgaagatgcc gccgacgcag gacgaggcca acgccgcccc cagggcgcgc ccggcctggc
     7321 cgctacgtgc catcgggtgg ccatcgacca cggtcgcggc atccgggccg ctgccgggca
     7381 caccgagcag gatgctggga atggggccgc cggtgtggat caccgaatgc atcgcgagca
     7441 ggaatacggc gcccacggtc ggatccgcgc ccaccaggaa gggaatggcc atcacgatcg
     7501 acagcttgcc acccaggccc ggaacaaccc cggccagcac gcccagcggc actgccacca
     7561 gcagcgccgc gagcaccgcg ggatcatcga cgtaggcgcc cagtccggcg agcgcatcgg
     7621 cgaatatcac gggccgggca ccgcctgcgc cagcaggcgc gagatccgct cctgcaccgc
     7681 cgcgccgtcc agcgcttcga tcggcagcct catcgccgcc gcctcacgca gcagctcggg
     7741 atcggcgagc gtgtcgagat acgcctcgcg cagcaccgcc agccgctcgg gcggcacgcc
     7801 tggcggcgcg acc
//
LOCUS       DAORUQ010000151         7805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10442_length_7805_cov_5.517419, whole genome shotgun sequence.
ACCESSION   DAORUQ010000151 DAORUQ010000000
VERSION     DAORUQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10442_length_7805_cov_5.517419"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..795
                     /locus_tag="PLW13_12190"
     CDS             1..795
                     /locus_tag="PLW13_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71183.1"
                     /translation="MSSMLDRFRLDGQVAIITGSGRGIGAACALAFADAGADVVLSAR
                     SLAELEETAARVRARGRRALVVPCDVMEAAQRDALVARAVQEFGRLDILVNNAGGWGP
                     KPALDTTDEDFETCFRFNVTTAFSMSRLSVPHMITTAGKGAIINISSMAGSHPQPGFV
                     AYGVGKAAMSWMTQELAQDFAPKVRVNAIGVGATRTTALTNFMTDDMERTMAARTPMA
                     RLGDPEDVAACALYLASPASAYVTGEVIGVNGGIVTSQVTMPRANI"
     gene            887..2962
                     /locus_tag="PLW13_12195"
     CDS             887..2962
                     /locus_tag="PLW13_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HQY71184.1"
                     /translation="MFRFVLAVGFSLAAAIAGAAPAPAAVDDARLRAADADTANWMSH
                     GRTYSEQRFSPLAQIDEGNVARLGLAWSQTLDFDRGVQATPLVVDGVMYLTGPWSVVY
                     AFDAATGTPLWQHDPQVDRAQAVEGCCGPVNRGVAVWEGRVYVGTLDGRLVALDAASG
                     KEAWSVDTLIDASRGYNITGAPRVVKGKVLIGNGGADMGARGYVSAYDAMTGAMAWRF
                     FTVPGDPAKGFENATMAMAAKTWSGEEWWKWGGGGTVWDAMSYDPELDLLYIGVGNSS
                     LYPRARRSPDGGDNLFVSSIVALRPDTGEYVWHYQLAPAEQWDYPATTQMVLLDIPFQ
                     GEMRKLLVQVPKHGFVYVLDRVTGKLLSAEKFTKVTWASGYDLASGRPIENAEADYSK
                     TGKAALVYPGPLGGHNWNPVSWNPGTGLLYFSEVQMPSVFKADNAVGFRERGRRYNMA
                     LDMAGMMDNPGFLAELKSPRGSLIAWDVANAKIAWQVPQPMPLNGGTLSTAGNLVFHG
                     TADGRLVAYAADSGKQLWEGHTFGGVQPGPVSYAVDGRQYIATGIGWGGGHGAAMPDG
                     GKLAGLTNASRIAVYTLDGKAALEPPAPFTGTIDAPPLSATPEQIERGAQLYMVHCGY
                     CHASGSGGVPDLSYMSRQTHSEFMAIVRGGLRLHKGMPAFTEQLTDEEAQAVHAFLIA
                     QAQRAKAGP"
     gene            complement(3017..3280)
                     /locus_tag="PLW13_12200"
     CDS             complement(3017..3280)
                     /locus_tag="PLW13_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQY71185.1"
                     /translation="MHWSVYIIESTTGTLYTGVSTDVQRRFREHAGGRRGARYFRSAK
                     PRSVVFVEDGHTRSSACRREAAIKRLSREGKLALIAARDLPLP"
     gene            complement(3284..3622)
                     /locus_tag="PLW13_12205"
     CDS             complement(3284..3622)
                     /locus_tag="PLW13_12205"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HQY71186.1"
                     /translation="MRLPLAALLALMPFAAHAGFDSGNRLYEDCGSENYFNRGYCGGY
                     ITGIVDTIEAMQQNGQLPKNTLCIPDNVTKGQLADAVKMYLGSNPSRRHLDAGSLVPE
                     ALKRSFPCGG"
     gene            complement(3702..>4487)
                     /locus_tag="PLW13_12210"
     CDS             complement(3702..>4487)
                     /locus_tag="PLW13_12210"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQY71187.1"
                     /translation="AARFCPQCGQKQGTGIPGALAYLHEIVNHYVALDGKLWRTLWLL
                     LARPGMLVTEYIAGRRQRYIGPLKLYLTFSVIFFVLASLAPAADVRVAVALEPGSAQA
                     RELEQALEQIPQGLRQTVERTLAEAERNTAQPAPAAREIGDRLQSQAPRAMFLLLPAF
                     AGLSLFAFRRRPQHYAVHLMLALHAHAVAFLLLILRLALPAAIGGKLALLLPLWLLFA
                     FRHVFGGRWWALAARAGFVSAGYALLLVATVAAGALLLAATPA"
     assembly_gap    4488..4587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4588..5211
                     /gene="queC"
                     /locus_tag="PLW13_12215"
     CDS             <4588..5211
                     /gene="queC"
                     /locus_tag="PLW13_12215"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQY71188.1"
                     /translation="FETHALSFRYGQRHVVELDCARTLAQVLGAARHVIADIDLRVFG
                     HSALTADIEVPKGRDPRHMDEDIPVTYVPARNTIFLSFATAWAEVLGAADIFIGVNAL
                     DYSGYPDCRPAFIEAFACMANLATRAGVEGTTELHIRTPLIELSKAAIIRLGTDLGVD
                     FALTSSCYDPVPDGRPCGGCDSCLLRARGFAEAGLADPLLARFAGTP"
     gene            5208..6275
                     /locus_tag="PLW13_12220"
     CDS             5208..6275
                     /locus_tag="PLW13_12220"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQY71189.1"
                     /translation="MNGTLLHVAAAPFEAAVSVPVLPQGHRSRRLHGHSFLAAVRSRD
                     GAALGERLREALAPLDYGYLNERITVPTDENIARWIRDALAGPAIETVSVQSTRHQGV
                     DIDRANHAHLWRRFRFEAAHRLPCVAPGHPCGRMHGHGFEVILHVNQDLAAADMGIDF
                     DRLGELWQPLHATLHLACLNDIAGLQNPTSEMLACWIWQRLHEVLPELSWVTVYETVT
                     AGCHYDGRHFRIWKEQRFESAVRLVNAAPGDARRRLHGHSYITRLHLSAPLDAMRGWT
                     IDYGEVREAFTPVFRELDHHALNESTGTDGDLPALLGWMREHVAPVLPALDRIDLYAT
                     PHTGAILHWGEEGPALPGQAP"
     gene            6272..6913
                     /gene="queE"
                     /locus_tag="PLW13_12225"
     CDS             6272..6913
                     /gene="queE"
                     /locus_tag="PLW13_12225"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HQY71190.1"
                     /translation="MSYAVKEIFYTLQGEGANAGRPAVFCRFAGCNLWSGREKHRAKA
                     VCRFCDTDFVGTDGPGGGRFADAPGLAQRIAAQWPREGVARARPLVVFTGGEPLLQLD
                     AALIEAVHALGMEIAVETNGTRPAPPGIDWLCVSPKAGAPLVLLAGDELKLVYPQAEA
                     PPERFAALAFHDFYLQPMDGPDYARNLAASVEYCLRHPQWRLSLQLHKLAGIP"
     gene            6910..>7805
                     /locus_tag="PLW13_12230"
     CDS             6910..>7805
                     /locus_tag="PLW13_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71191.1"
                     /translation="MKTLDTRFYTGHNVYAATTVAFRQFELEPAELDSLAGLGERLSR
                     ELGAELARAGVIDAPAAFPALPPGAQARALFAHCLVHTAIVLQRCSRHRVAYAEWRLE
                     DDSRRVVALFEFEALEVGQRAAELALALLCHCWHALDRDIAAPADATPLAQHVLSFAR
                     LAVPLVLPRDTQAIVDAARRAGIPYLKMDRFPFEPRQGAFRIRPNGLLKLGHCCHQHI
                     VDGTFCVDRSNAVAPLLRDREQILALLHALGLPLARRDPQQGNCATLSRAQRVASRIG
                     YPVVLRPLVRERGDAPAPAIRN"
BASE COUNT         1155 a         2629 c         2780 g         1141 t
ORIGIN      
        1 atgagcagca tgctggatcg ctttcgcctc gatggccagg tcgccatcat caccggttcc
       61 gggcgcggca ttggcgcggc ctgtgcgctg gcattcgccg atgccggcgc cgacgtggtg
      121 ctctccgcgc gctctctggc cgagctcgag gaaaccgcgg ccagggtgcg cgcacgcggg
      181 cggcgcgcgc tggtggtgcc ctgcgacgtc atggaggcag cccagcgcga tgcgctggtc
      241 gcgcgtgcgg tgcaggaatt cggccggctc gacatcctgg tgaacaacgc cggcggctgg
      301 ggcccgaaac cggcgctgga taccacggac gaggacttcg agacctgttt tcgcttcaac
      361 gtgaccacgg cgttttcgat gtcgcggctc agcgttccgc acatgatcac gacggccggc
      421 aagggcgcga tcataaacat ctcctcgatg gccggcagtc atccgcagcc gggcttcgtg
      481 gcctacggcg tgggcaaggc ggcgatgtcg tggatgacgc aggagctcgc gcaggacttc
      541 gcgcccaagg tgcgcgtcaa tgccatcggc gtgggagcca cgcgcaccac ggcgctgacc
      601 aatttcatga ccgacgacat ggagcgcacg atggccgccc gtacgccgat ggcacgtctc
      661 ggcgacccgg aggatgtcgc ggcctgcgcg ctctacctcg catcgcccgc ctcggcctac
      721 gtgaccggcg aggtgatcgg cgtcaacggc ggcatcgtga cctcgcaggt gacgatgccg
      781 cgcgcgaaca tctgagcgca gcgcgcgccc ggattaccgc taattaacgc aggccccggc
      841 gcggatctgg aatgatgcgc cgctgaaaat ctgccggaga tcattgatgt tcaggtttgt
      901 gctcgccgtg ggtttctcgc tcgccgccgc catcgccggc gcggccccgg cgccggctgc
      961 ggtcgatgac gcgcgtctgc gcgcggccga cgccgatacc gccaactgga tgagccacgg
     1021 tcgcacctat tccgagcagc gcttcagccc gctggcgcag atcgatgaag gcaacgtggc
     1081 acggctcggg ctggcgtgga gccagacgct cgatttcgac cgcggcgtcc aggccacgcc
     1141 gctggtggtc gatggcgtga tgtacctcac cgggccgtgg agcgtggtgt atgccttcga
     1201 tgccgccacc ggcacgccgc tgtggcagca cgatccccag gtggatcgcg cgcaggccgt
     1261 ggaaggctgc tgcggtccgg tgaaccgcgg cgtcgcggtg tgggaagggc gcgtgtacgt
     1321 cggcacgctc gacggccgcc tggtcgcgct cgatgccgcg agcggcaagg aagcgtggag
     1381 tgtcgacacg ctgatcgacg cctcgcgcgg ctacaacatc accggcgcgc cgcgcgtcgt
     1441 gaagggcaag gtgctgatcg gcaacggcgg cgccgacatg ggcgcgcgcg gctatgtctc
     1501 ggcctacgac gcgatgacgg gcgcgatggc atggcgcttc ttcaccgtcc cgggtgatcc
     1561 cgcgaagggc ttcgagaacg cgaccatggc gatggccgcg aagacctgga gcggcgagga
     1621 gtggtggaag tggggcggcg ggggcacggt gtgggacgcg atgtcctacg acccggaact
     1681 ggacctgctc tacatcggtg tcggcaacag cagcctgtat ccacgcgcgc ggcgcagccc
     1741 ggacggcggt gacaacctgt tcgtgtcctc gatcgtggcg ttgcgccccg acacgggcga
     1801 atacgtctgg cactaccagc tcgcgcccgc cgagcagtgg gattacccgg ccacgacgca
     1861 gatggtgctg ctggatatcc cgttccaggg cgagatgcgc aagttgctgg tgcaggtgcc
     1921 caagcacgga ttcgtctacg tgctggatcg cgtcaccggc aagctgctgt cggcggagaa
     1981 gttcacgaaa gtgacctggg ccagtggcta cgatcttgcg agcggccgcc cgatcgagaa
     2041 cgccgaagcg gattacagca agaccggcaa ggcggcgctg gtgtacccgg gtccgctcgg
     2101 cggacacaac tggaacccgg tgtcgtggaa ccccggcacc ggcctgctgt atttcagcga
     2161 agtgcagatg ccgagcgtgt tcaaggccga caacgcggtg ggttttcgcg agcgcggacg
     2221 tcgctacaac atggcgctgg acatggcggg gatgatggac aacccggggt ttctcgccga
     2281 gctgaagagc ccgcgcggtt cactgatcgc ctgggacgtg gcgaacgcga aaatcgcctg
     2341 gcaggtgccg cagccgatgc ccctgaacgg cggcacgctg agcaccgcgg gcaacctggt
     2401 gttccacggc accgccgacg ggcgcctggt ggcctacgcg gccgacagcg gcaagcaact
     2461 gtgggaaggc cacacctttg gtggcgtgca gcccggcccg gtcagctacg cggtggacgg
     2521 gcgccagtac atcgccaccg gtatcggctg gggcggcggt cacggcgcgg cgatgcccga
     2581 cgggggcaag ctggccggac tgacgaatgc ctcgcgcatc gcggtgtaca cgctcgacgg
     2641 caaggcggcg ctggagccgc ccgcgccatt caccggcacg atcgatgcgc cgccgctgag
     2701 cgcgacaccg gagcagatcg agcgcggcgc gcaactgtac atggtgcatt gcggctactg
     2761 ccacgcctcg ggcagcggtg gcgtgccgga cctcagctac atgagccgac agacgcacag
     2821 cgagttcatg gcgatcgtgc gcggcggcct gcgcttgcac aagggcatgc cggcgttcac
     2881 cgagcagttg acggacgagg aggcgcaagc ggtacatgcc ttcctgatcg cgcaggcgca
     2941 gcgcgcgaaa gccgggccgt aggtccgcat tcatgcggac atttcccggg cggacatcgt
     3001 ccggctgaag ccggacctac gggagcggga gatcgcgcgc ggcgatcagg gccagcttgc
     3061 cttcgcggct caggcgcttg atcgccgcct cgcgacggca ggcgctggaa cgcgtgtggc
     3121 cgtcttccac gaacaccacg gacctgggct tcgcgctgcg gaagtagcgc gcgccgcggc
     3181 gtcccccggc gtgctcgcga aagcgtcgct gcacgtcggt gctgacgccg gtgtagagcg
     3241 tgcccgtggt gctctcgatg atgtagaccg accagtgcaa ggctcaaccg ccgcagggga
     3301 atgagcgctt gagcgcctcg ggcaccaggc tgccggcatc gaggtggcga cgcgaggggt
     3361 tgctgccgag gtacatcttc accgcgtcgg cgagctgtcc cttggtgacg ttatcgggga
     3421 tgcacagcgt gttcttcggc agctgcccgt tctgctgcat ggcctcgatg gtatcgacga
     3481 tgccggtgat gtagccgccg cagtagccgc ggttgaagta gttctccgag ccgcagtcct
     3541 cgtagaggcg gttgccggag tcgaaacccg cgtgcgcggc gaagggcatc agggcgagga
     3601 gggcggcgag cggaaggcgc atggtggctc cggtggtcgg ttgggatcgg gcgctactct
     3661 agtgcaggag cgcggggctc gccaatgcgc cggggaccgt ttcaggccgg cgtggcggcg
     3721 agcagcagcg cgccggccgc gaccgtggcg accagcagca gcgcatagcc cgcgctcaca
     3781 aagccggcgc gcgcggccag cgcccaccag cgtccgccga agacgtggcg aaaggcgaac
     3841 agcagccaca gcggcagcag cagcgccagc ttgcccccga tggcggccgg cagcgccagc
     3901 cgcaggatca gcaacaggaa ggccaccgcg tgcgcgtgca gcgcgagcat gaggtggacc
     3961 gcgtagtgct gcgggcggcg gcggaatgcg aacagcgaga gtcccgcgaa cgcgggcagc
     4021 agcaggaaca tcgcgcgcgg cgcctgcgac tgcaggcgat cgccgatctc gcgtgcggcc
     4081 ggcgccggct gcgccgtgtt gcgctcggcc tcggcgagcg tgcgttcgac cgtctggcgc
     4141 agtccctgcg ggatctgctc cagcgcctgc tcgagctccc gggcttgcgc gctgcccggc
     4201 tcgagcgcga cagccacgcg gacatcggcc gcgggcgcca gcgaggcgag cacgaagaag
     4261 atcacggaga aagtcaggta gagcttgagc gggccgatat agcgctggcg gcgcccggcg
     4321 atgtactcgg tgaccagcat ccccgggcgg gcgagcaaca gccacagcgt gcgccagagc
     4381 ttgccatcga gcgcgacgta gtggttcacg atctcgtgca ggtaggcgag cgcgccgggt
     4441 attcccgtgc cctgcttctg gccgcattgc gggcaaaaac gcgccgcnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnttc gagacccatg cgctgtcgtt ccgctacggg
     4621 cagcgccacg tcgtggagct cgattgcgcg cgcacgctgg cgcaggtgct cggcgcggcg
     4681 cggcacgtga tcgccgacat cgacctgcgc gtgttcgggc actcggcgct gaccgcggac
     4741 atcgaggtcc ccaaggggcg cgacccgcga cacatggacg aggacatccc ggtcacctac
     4801 gtgcccgcgc gcaacaccat cttcctgtcc ttcgcgacag cctgggccga ggtgctgggc
     4861 gcggcggaca tcttcatcgg cgtgaacgcg ctcgactact cgggctaccc ggattgccgg
     4921 ccggcgttca tcgaggcttt cgcgtgcatg gcgaacctgg cgacgcgcgc cggcgtcgag
     4981 ggaacgaccg agctgcacat ccgcaccccg ctgatcgagc tcagcaaggc ggcgatcatc
     5041 cgcctcggca cggatctcgg ggtggatttc gcgctcacca gcagctgcta cgacccggtg
     5101 cccgacgggc gaccctgcgg cggttgcgat tcctgcctgc tgcgggccag gggcttcgcc
     5161 gaggcgggcc tggcggaccc gctgctcgcg cgtttcgcgg ggacgccgtg aacggcacgc
     5221 tgctgcacgt ggccgccgcg cccttcgagg ctgccgtcag cgtgccggtg ctgccgcagg
     5281 ggcatcgttc gcggcgcctg cacgggcaca gcttccttgc cgcggtgcgt tcgcgcgacg
     5341 gcgcggcgct cggcgagcgc ctgcgcgagg cgctcgcgcc gctcgattac ggctatctca
     5401 acgagcgcat cacggtgccc accgacgaga acatcgcgcg ctggatccgc gacgcgctcg
     5461 ccgggcccgc gatcgagacg gtcagcgtgc agagcacgcg ccaccagggc gtcgacatcg
     5521 accgcgccaa ccacgcgcac ctgtggcggc gctttcgctt cgaggcggcg caccggttgc
     5581 cgtgtgtggc acccgggcat ccctgcggtc gcatgcacgg tcacggattc gaggtgatcc
     5641 tgcacgtgaa ccaggacctc gccgcggccg acatgggcat cgatttcgac cgcctgggcg
     5701 agctgtggca gccgctgcac gcgacgctgc accttgcctg cctgaacgac atcgcgggac
     5761 tccagaatcc caccagcgag atgctcgcct gctggatctg gcagcggctg cacgaggtgc
     5821 tgcccgagct gtcgtgggtc acggtctacg agaccgtcac cgcgggctgc cactacgacg
     5881 ggcgccattt ccgcatctgg aaggagcagc gcttcgagag cgcggtgcgg ctcgtgaacg
     5941 ccgcacccgg tgatgcgcgc cggcggctgc acgggcacag ctatatcacg cgcctgcacc
     6001 tgagcgcgcc gctggacgcc atgcgcggct ggaccatcga ctacggcgag gtgcgcgagg
     6061 ccttcacgcc ggtgttccgc gagctcgacc accacgcgct gaacgaatcc accggcaccg
     6121 acggcgacct gccggcgctg ctgggctgga tgcgcgagca cgtcgcgcca gtgctgccgg
     6181 cgctggatcg catcgatctc tacgccacgc cgcacacggg cgcgattctg cactggggcg
     6241 aggaggggcc ggcgctgccg gggcaggcgc catgagttac gcggtcaagg aaatcttcta
     6301 cacgctgcag ggcgagggcg ccaacgccgg gcgcccggcg gtgttctgcc gcttcgccgg
     6361 ctgcaacctg tggtcgggac gcgagaagca ccgcgcgaag gcggtgtgcc ggttctgcga
     6421 taccgacttc gtgggcacgg acggccccgg cggcgggcgc ttcgccgatg cgccgggcct
     6481 ggcgcaacgc atcgccgcgc aatggccgcg cgagggggtg gcgcgcgcgc gcccgctggt
     6541 cgtgttcacc ggcggcgagc cgctgctgca actcgacgcg gcgctgatcg aggcggtgca
     6601 cgccctgggc atggagatcg cggtcgagac caacggcacg cggcccgcgc ccccgggcat
     6661 cgactggttg tgcgtctcgc ccaaggccgg cgcgcccctc gtgctcctgg caggcgacga
     6721 gctgaagctg gtgtacccgc aggccgaagc gccgcccgag cgcttcgccg cgctcgcctt
     6781 ccacgatttc tacctgcagc cgatggacgg ccccgactac gcgcgcaacc tcgccgcgag
     6841 cgtcgagtac tgcctgcgcc acccgcaatg gcggctgagc ctgcaattgc acaagctggc
     6901 cgggatcccg tgaagacgct ggatacccgc ttctacaccg ggcacaacgt ttacgccgcg
     6961 accaccgtgg cgttccggca gttcgagctg gaaccggcgg agctcgattc gctcgcgggg
     7021 ctcggggagc gactgtcgcg cgagctgggc gccgagctcg cgcgcgccgg cgtcatcgac
     7081 gcgccggcgg cgtttccggc gctgccgccc ggcgctcagg cgcgcgcgct gttcgcacac
     7141 tgcctcgtcc acaccgcgat cgtgctgcag cgctgctcgc gccatcgcgt tgcgtatgcc
     7201 gagtggcggc tcgaggacga tagccggcgc gtggtggcgc tgttcgaatt cgaggcgctc
     7261 gaggtgggcc agcgcgcggc ggagctcgcg ctggcgctgc tttgccattg ctggcacgcg
     7321 ctcgatcggg atatcgccgc accggcggac gccacgccgc tcgcgcagca cgtgctttcc
     7381 ttcgcgcgcc tcgcggtgcc actggtcttg ccccgcgaca cgcaggcgat cgtcgatgcc
     7441 gcgcggcgcg ccggcatccc gtacctgaag atggaccgct tccccttcga gccccggcag
     7501 ggcgcgttcc gcatccgccc caacggtctg ctgaagctgg ggcactgctg ccaccagcac
     7561 atcgtcgacg gcactttctg cgtcgatcgc tcgaacgccg tcgcgccgct gttgcgcgat
     7621 cgcgagcaga tcctcgcgct gctgcacgcc ctgggcctgc cgctggcgcg gcgcgacccg
     7681 cagcaaggca actgcgcgac cctgagccgc gcacagcgcg tggcctcgcg catcggctac
     7741 ccggtggtgc tgcggccgct cgtgcgcgag cgtggcgacg ccccggcgcc ggccatccgc
     7801 aacga
//
LOCUS       DAORUQ010000152         7785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10490_length_7785_cov_5.579948, whole genome shotgun sequence.
ACCESSION   DAORUQ010000152 DAORUQ010000000
VERSION     DAORUQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10490_length_7785_cov_5.579948"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLW13_12235"
     CDS             <1..722
                     /locus_tag="PLW13_12235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008004553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71192.1"
                     /translation="GAGQGVGQGIAFALAAEGARVAVTGRTLAKLENTVAEIERRGGT
                     ALPVQCEVKSLESLQSCVKTVADHFGGINILVNNAQEVPNGELFKVSDEAFEAGFQSG
                     PLATMRLMKLCYPYLKGDGSIVNLASSATKRWDMSGYGAYAAVKDAIRQLTRAAACEW
                     GKDNVRTNVILPHASSPGLVWWMEKNPEEAAAFFKTLPMGRVGDCEQDIGRFVAVLCS
                     DYSRYVNGQAIGLDGGQANIG"
     gene            747..1484
                     /locus_tag="PLW13_12240"
     CDS             747..1484
                     /locus_tag="PLW13_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQY71193.1"
                     /translation="MEIYGHRGAAGEAPENTLGGFEHAWERGVRRFELDVQLAADEQL
                     VVLHDARVNRTTGRSGAVDALTSRELGAMDARRGAPPWPRREGVPTLEAVMRAFPRTE
                     SWQLEVKKPARADRLRITLEALHELVTRLRLGPRVIVTSSNTAVLDMARQVAPRLARG
                     YVCTKMDPLPIAEEFGCRMVVANLFTCTPGLRLGAWRRGIELSAWTVNDPISIRNLYR
                     MRAHSIITDYPSMALPLVGQLGGGRAA"
     gene            1495..2079
                     /locus_tag="PLW13_12245"
     CDS             1495..2079
                     /locus_tag="PLW13_12245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY71194.1"
                     /translation="METLPRLRNRYFALRHGSSEANARGIIVSDPRTGEDGYGLTAAG
                     RTEVRDALTGWHEDVALIVSSPFRRASESASIARTVLGGEAPLVIDARLRERHFGALE
                     GQRTTRYEEIWSADAKDPHHCRHGAETAAAVRSRAVDLILDLEREHAELCILLVAHGD
                     TLQLLWTAFARRDAAAHRQRPPLLPAGLEEFRQH"
     gene            2116..2976
                     /locus_tag="PLW13_12250"
     CDS             2116..2976
                     /locus_tag="PLW13_12250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HQY71195.1"
                     /translation="MEYWLGIFHEDNEQMKTLARAAEQMGFAGIALPDHVAMPAGYKA
                     VHPSGRRHIEHDTRFPCPFITAANITAVTERLRVMTYIYVLPMREPFTVAKQVATLCA
                     QSDYRFDFGIGAGWCEDEFRIMGPDFGTRGARMDEMLSIMRQFWETGECAFEGSHFSF
                     PTVGQQPRPARRIPVWIGGKSRAALRRAARNDGWLGMNYGMEEIHALLARLEEERARY
                     LDGGGTDHGSFRRFVIPETTPSHDVYRQLEDWGVDGTVALCWGIDDPAYFPLEKKLEG
                     MEAFARTYLR"
     gene            3216..4184
                     /locus_tag="PLW13_12255"
     CDS             3216..4184
                     /locus_tag="PLW13_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQY71196.1"
                     /translation="MDFKDYYRILGVPDTADEKEIKASYRKLARKYHPDVSKERDAEN
                     RFKELGEAYEVLKDPEKRARYDQLRRLGAQQPDGGFRPPPGWQSAGGFYEGSFSGDDA
                     RQFSDFFESVFGRARTARDGFAHEGGAHGVRMRGEDIHHTISLFLEEAYRGCEHQIRL
                     LVPEIDESGSVSRREKTLNIKIPPGVTPGQRIRLAGQGGPGIGGAAAGDLFLEVQLAP
                     HPYFSVDGRNILLTLPVASWEAALGATVEVPTLGGKVKLKIPGGSSSGDRLRIKGKGL
                     PGKRAGDQLVVLEVTLPRHHGSVAESLYRQIAVQESTFNPRSAWED"
     gene            4188..4520
                     /locus_tag="PLW13_12260"
     CDS             4188..4520
                     /locus_tag="PLW13_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HQY71197.1"
                     /translation="MATPVIYFAELQDDGSLFSLGEICERCGVPADIVVEMVEYGIVT
                     PVTDLTNNDHWQFDTEALLRMSRAQRLRRDLELDPAGLALALELLDEIDTLQRQVRTL
                     QQQLRHAR"
     gene            4551..5684
                     /locus_tag="PLW13_12265"
     CDS             4551..5684
                     /locus_tag="PLW13_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQY71198.1"
                     /translation="MIKSYFVTPVRFDFGARALLPKELKALGIARPLLITDPGMVATG
                     TAARVLELMPAAAAMFSDLPGNPNADNVDAAVAAYRAGSHDGLVALGGGSAIDCAKGA
                     AVMATHPGELRDYDIMKVIRREGRAITPAAAPIVAIPTSAGTGSEVSTIAVLSVGPGE
                     KIVLINNNLLPRVTLADPELTLSLPRGLTAGTGMDALSHCLEAYFSSNENPPAKAVAL
                     DGITRIVANIRTAYNEPQNRQARWEMMMGALEGGMTMLNGCGAMHAMAHAIGGRHDLA
                     LHHGTLNAVLMPVVLELNRPHCTALFARLEKTLELPGGTNFIDVVRRLNADLDIPRGL
                     GAMGVTAAMIPALAEAAVRDVNTLTNPVRLDAAGYEGLLRAAM"
     gene            complement(5747..6979)
                     /locus_tag="PLW13_12270"
     CDS             complement(5747..6979)
                     /locus_tag="PLW13_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQY71199.1"
                     /translation="MQLLRLLGPGILVAATGVGAGDLATASFAGSQLGAAILWAAVLG
                     AFLKFVLNEGLARWQLATGDTLLEGAVLRFGRIVGWLFLPYLFLWSFFVGAALMSACG
                     AALQALFPLFDDAERGRMVFGALSSLLGLVLVRAGGFRLFERVMSVCIGVMFATVVVM
                     AVMLWPGTTEVLRGLLLPGIPDLRGEGVVWTVALMGGVGGTLTVLCYGYWIREEGRAH
                     IGDLPLCRIDLGLGYAMTALFAIAMVIIGSQVQIDGRGTGLLLALADTLGAELGEAGR
                     WLFLVGVLGAVMSSLLGVWQAVPYLFADLWSMTFARHEPGAVRPAVDMRAAPYRVYLW
                     LLALAPMLGLVLSFKEIQKLYATMGATFIPLLALALLLMNGRRAWVGAHVNRWPTVIV
                     LLATLLFFAALGWFRIGE"
     gene            complement(6986..7705)
                     /locus_tag="PLW13_12275"
     CDS             complement(6986..7705)
                     /locus_tag="PLW13_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQY71200.1"
                     /translation="MDSRNTLGALLLGIAIAIGLAALGYQLGNAAIRFKEYERFVAVK
                     GLSEHEYPADIVIWPIQFSAADNDLESLYASIEANTASIREFLLAQGIGADAISVAPP
                     SITDKSAQRFGDGQRAEFRYTADQIVTVYSAEVGQVRGVMDKLAQLGKQGIAFAGGEY
                     ANQPEYLFTRLNEVKPGMIEEATMKAREVAAKFAADSGSRLGKIRQASQGQFSIEARD
                     RNNPHIKRVRVVSTVEYYLSD"
BASE COUNT         1363 a         2682 c         2652 g         1088 t
ORIGIN      
        1 ccggcgccgg ccagggcgtg ggccagggca tcgccttcgc gctcgccgcc gaaggcgccc
       61 gcgtggcggt taccggccgt acgctggcga aactcgagaa caccgtcgcc gagatcgagc
      121 gccgcggtgg cacggcgctc ccggtgcaat gcgaggtgaa gagcctggag tcgctgcagt
      181 cctgcgtgaa gaccgtggcc gatcacttcg gcggcatcaa catcctggtc aacaacgcgc
      241 aggaagtgcc caacggagag ctgttcaagg tgagcgacga ggccttcgag gccggcttcc
      301 agtcgggtcc gctcgcgacc atgcgcctga tgaagctctg ctacccgtac ctgaagggcg
      361 acggctcgat cgtgaacctg gcgagctcgg ccaccaagcg ctgggacatg tcgggctacg
      421 gcgcctacgc cgccgtgaag gacgccatcc gccagctcac gcgtgccgcc gcctgtgaat
      481 ggggcaagga caacgtccgc accaatgtca tcctgccgca cgcgagctcg ccggggctcg
      541 tgtggtggat ggagaagaac cccgaggagg ccgcggcatt cttcaagacg ctgccgatgg
      601 ggcgcgtggg cgattgcgag caggacatcg gccgcttcgt ggcggtgctc tgctcggact
      661 actcgaggta cgtcaacggc caggccatcg gcctcgacgg cggccaggcg aacatcggct
      721 gagcccgggg cacaggtagc gcgcgcatgg agatttacgg gcatcgcggc gccgccggcg
      781 aggcgcccga gaacacgctc ggcggtttcg agcacgcctg ggaacgcggc gtgaggcgct
      841 tcgagctcga cgtgcagcta gccgccgacg agcaactggt cgtgctgcac gacgcgcgcg
      901 tgaatcgcac gaccgggcgc agcggcgcgg tcgatgccct caccagccgc gagctcggcg
      961 ccatggatgc gcggcgcggc gcgccgccgt ggccgcggcg cgagggggtg cccacgctcg
     1021 aggccgtgat gcgcgccttc ccgcgcacgg agtcgtggca gctcgaagtc aagaagcctg
     1081 cccgcgccga ccgcctgcgc atcaccctgg aggcactgca cgagctggtg acgcggctgc
     1141 ggctcggccc gcgcgtcatc gtcacgtcgt cgaacaccgc ggtgctggac atggcgcgcc
     1201 aggtggcacc acgcctggcg cgcggctatg tctgcacgaa gatggacccg ttgccgattg
     1261 ccgaggaatt cggctgtcgc atggtcgtgg ccaacctctt cacctgcacg ccggggctgc
     1321 gcctcggcgc ctggcggcgc gggatcgagc tctccgcgtg gaccgtgaac gacccgatct
     1381 cgatccgcaa cctctaccgc atgcgcgcgc acagcatcat cacggactac ccgtcgatgg
     1441 cgctgccgct ggtcggccag ctgggcggcg gaagggccgc ctgagcgcgg gcgcatggag
     1501 acgctcccac ggctgcgcaa ccgctacttc gcgctgcgtc acggcagcag cgaggccaat
     1561 gcgcgcggca tcatcgtcag cgacccgcgc accggcgagg acggttacgg cctcaccgcg
     1621 gcgggacgaa cggaggtgcg cgacgcgctc accggctggc acgaggacgt ggcgctgata
     1681 gtcagctcgc cattccggcg cgccagcgag agcgcctcga tcgcgcgaac cgtgctcggc
     1741 ggcgaggcac cgctcgtgat cgatgcccgc ctgcgcgaac gtcacttcgg cgcgctcgag
     1801 ggccagcgca ccacgcgcta cgaagagatc tggagcgccg acgccaagga cccgcaccac
     1861 tgccggcacg gcgccgaaac cgccgccgcg gtgcgctcac gcgccgtcga cctgatcctg
     1921 gacctggaac gcgagcacgc cgagctctgc atcctgctgg tggcgcacgg cgacaccctg
     1981 cagttgctgt ggaccgcgtt cgcccgccgc gatgcggcgg cgcatcgcca gcgaccaccc
     2041 ctgcttcccg cagggctcga ggaattccgg cagcactgag tgctgccatc acgacaacgc
     2101 aacaggagag cgaaaatgga atactggctg ggcatcttcc acgaagacaa cgagcagatg
     2161 aagaccctgg cgcgtgccgc ggagcagatg ggattcgccg gcatcgcatt gcccgaccac
     2221 gtggcaatgc ccgccggcta caaggccgtg cacccctcgg ggcggcgcca catcgagcac
     2281 gatacgcgct tcccctgtcc gttcatcacg gccgcgaaca tcacggccgt gaccgaacgc
     2341 ctgcgcgtga tgacctatat ctacgtgctg ccgatgcgcg aacccttcac ggtcgccaaa
     2401 caggtggcca cgctttgcgc ccagtcggac taccgcttcg acttcggcat cggcgccggc
     2461 tggtgcgagg acgagttccg gatcatgggg ccggacttcg gcacccgtgg cgcgcgcatg
     2521 gacgaaatgc tgtcgatcat gcgccagttc tgggaaaccg gcgagtgcgc cttcgagggc
     2581 agtcacttca gtttccccac cgtgggccag cagccgcggc cggcgcgacg cattccggtg
     2641 tggatcggcg gcaagtcacg cgccgcgctg cgccgcgccg cgcgcaacga cggctggctg
     2701 ggcatgaact acgggatgga ggagatccat gcgctgctgg cccggctcga ggaggagcgc
     2761 gcgcgctacc tggatggcgg cggcaccgac cacggcagtt tccgccgctt cgtgattccc
     2821 gagacgacgc cctcgcacga tgtgtaccgg caactggagg attggggggt cgatggcacc
     2881 gtggcattgt gctgggggat cgacgatccg gcgtacttcc cgctcgagaa gaaactcgag
     2941 gggatggaag cgtttgcccg cacctacctg cgctgattgc cgcgccgggc caggactgcc
     3001 catgtgaccg ggtaaatgtg tcgcgcgcgt ggtgttatgc tgcgccgagc gcaggatcaa
     3061 ccacgagagg aaaccgacat ggcaaaaggc atggacaaga agaaggaaac caagaaaaag
     3121 ccggccaaga cgcagaagga aaagcgggcc gacaaggcag ccaggaaggg ctgaggcgcc
     3181 tggcgggcag gaatcgcgcc ggggagttcc gctgaatgga cttcaaggac tactacagga
     3241 tcctcggcgt gcccgacacg gccgacgaga aggagatcaa ggccagttac cgcaagctgg
     3301 cgcggaaata ccatcccgac gtcagcaagg agcgcgatgc cgagaaccgc ttcaaggagc
     3361 tcggcgaggc ctacgaggtc ctgaaggacc cggaaaagcg cgcccggtac gaccagctgc
     3421 gcaggctggg tgcgcaacaa cccgacggcg gctttcgccc cccgccaggc tggcaatcgg
     3481 caggcgggtt ttacgagggc agcttcagcg gcgacgacgc acggcaattc agcgatttct
     3541 tcgagtcggt tttcggtcgg gcccgcaccg cgcgggacgg gttcgcgcac gagggcggcg
     3601 cacatggcgt gcgcatgcgc ggcgaggaca tccaccacac gatttcgctg ttcctggagg
     3661 aagcctaccg cggctgcgaa catcagatca ggctgctcgt cccggagatc gacgaatccg
     3721 gctcggtctc ccggcgcgaa aaaacgctga acatcaagat cccgccgggc gtgacccccg
     3781 ggcagcgcat ccgcctggcc ggccagggcg gcccgggcat cggcggggcc gcggccggcg
     3841 atctgttcct cgaggtccag ctcgcgccgc acccttattt cagcgtcgat ggccgcaata
     3901 tcctcctcac gctgccggtg gccagctggg aggccgcgct gggcgcgacg gtggaggtgc
     3961 cgacgctcgg cggcaaggtc aagctgaaga tacccggggg ctcgagcagc ggcgaccggt
     4021 tgcgcatcaa gggcaagggc ttaccgggca agcgcgcggg agatcaactg gtcgtgctcg
     4081 aggtcaccct gccgcgacac cacggctccg tggccgaatc actctatcgc cagatcgccg
     4141 tgcaggaatc cacgttcaac ccgcgctccg cctgggagga ctagggcatg gccacacccg
     4201 tgatctactt cgccgaactg caggacgacg gctcgttgtt cagtctcggc gagatctgcg
     4261 agcgttgcgg cgtccctgcc gacatcgtgg tcgagatggt cgaatacggc atcgtcactc
     4321 ccgtcaccga cctgaccaac aacgatcact ggcagttcga taccgaagcg ctgttgcgca
     4381 tgagccgcgc acaacgcctg cgccgcgacc tcgagttgga cccggccggg ctcgccctgg
     4441 cgctggaatt gctggatgaa atcgacacgc tgcaacgcca ggttcgcacg ctgcagcagc
     4501 agctgcggca cgcccgctag gcctgctcac tcgtcaccct ggaggcgccc gtgatcaaga
     4561 gctacttcgt gaccccggta cgctttgact tcggcgcccg cgcgctgctg ccgaaggagc
     4621 tgaaagcgct cggcatcgcg cgtccgctgc tgatcaccga tccgggcatg gtcgccaccg
     4681 gcaccgcggc gcgcgtgctc gagctgatgc cggccgcagc cgcgatgttc agcgacctgc
     4741 cgggcaaccc gaacgccgac aacgtcgatg cggccgtggc cgcgtaccgc gcgggctcgc
     4801 acgacggcct ggttgcgctg gggggcggct cggcgatcga ctgcgccaag ggtgccgcgg
     4861 taatggccac gcacccgggg gagctgcgcg actacgacat catgaaggtg atcaggcgcg
     4921 agggacgcgc gatcacgccg gccgcggcgc cgatcgtcgc gatcccgacc tcggcgggga
     4981 ccggctccga agtgtcgacg atcgcggtgc tgagcgtggg gccgggcgaa aagatcgtgc
     5041 tgatcaacaa caacctgctg cccagggtca cgctggccga ccccgagctc acgctctcgc
     5101 tgccgcgcgg gctgaccgcg ggcaccggca tggacgcgct gtcgcactgc ctcgaagcct
     5161 acttctccag caacgagaat ccgccggcca aggcagtcgc tctggacggc atcacgcgca
     5221 tcgtcgcgaa catccgcacc gcgtacaacg agccgcagaa ccgccaggcg cgctgggaga
     5281 tgatgatggg cgccctcgag ggcggcatga cgatgctcaa cggctgcggc gcgatgcacg
     5341 cgatggcgca cgcgataggc ggccggcacg atctcgccct gcaccacggc acgctgaacg
     5401 cggtgctgat gccggtggtg ctggaactca accgcccgca ctgcacggcg ctgttcgcgc
     5461 gcctcgaaaa gactctggag ctgcccggcg gcacgaactt catcgacgtg gtgcgccgcc
     5521 tgaacgcgga cctggacatc ccgcgcggcc tgggtgcgat gggcgttacc gcggccatga
     5581 tcccggcact cgccgaagcg gcggtacgcg acgtcaacac gctcaccaac ccggtgcggc
     5641 tcgacgcggc gggctacgag ggccttttgc gcgccgcgat gtaggtccgg cttcagccgg
     5701 gcattcggct ggattgcgcg caaaacatgt ccggataaat ccggacctac tcaccgatgc
     5761 ggaaccagcc gagtgcggcg aagaacaaca gcgtcgcgag cagcacgatc acggtgggcc
     5821 agcggttcac gtgcgccccc acccaggcgc gccgcccgtt catcagcagc agcgccagcg
     5881 cgagcagcgg gatgaaggtc gctcccatcg tcgcgtagag tttctggatt tccttgaagc
     5941 tcagcaccag ccccagcatg ggcgccagcg cgagcagcca caggtagacg cgatacggcg
     6001 ccgcgcgcat gtccacggcc ggcctcacgg cgccgggctc gtgacgggcg aatgtcatcg
     6061 accacaggtc cgcgaacagg tagggcaccg cctgccacac accgagcagg ctcgacatca
     6121 ccgcgcccag cacgccgacc aggaacagcc agcgtcccgc ttcgccgagc tcggcgccca
     6181 gcgtatcggc cagtgccagc agcaacccgg tgccgcggcc gtcgatctgc acctggctgc
     6241 cgatgatcac catcgcgatc gcgaacagcg cggtcatcgc atagccgagg cccagatcga
     6301 tgcggcatag cggcaggtca ccgatgtgcg cgcggccctc ctcgcggatc cagtagccgt
     6361 agcacagcac cgtgagcgtg ccacccacgc cgcccatcag cgcgaccgtc cacaccacgc
     6421 cctcgccgcg caggtccgga atgcccggga gcagcaggcc gcgcagcacc tcggtggttc
     6481 cgggccagag catgacggcc atcaccacca cggtcgcgaa catcacgccg atgcagacgc
     6541 tcatcacgcg ctcgaacagg cgaaaaccgc cggcgcgcac cagcacgagg ccgagcaggc
     6601 tggacagcgc gccgaagacc atgcggcctc gctccgcgtc atcgaacagc gggaacagcg
     6661 cctgcagggc tgcgccgcag gcgctcatca gcgcggcgcc cacgaagaac gaccagagga
     6721 acaggtaggg caggaacagc cagccgacga tgcgcccgaa gcgcagcacc gcgccctcga
     6781 gcagggtgtc gccggtggcg agctgccagc gcgcgagccc ttcgttcagc acgaacttga
     6841 ggaaagcacc gagcaccgcg gcccacagga tcgcggcgcc cagctggctg cccgcgaacg
     6901 aggctgtcgc cagatcgccg gcgcccacgc cggtggccgc gacgaggatg cccggcccca
     6961 gcagtcgcag cagctgcacg cgcgatcagt ccgagaggta gtactcgacc gtagacacca
     7021 cgcgcaccct cttgatgtgc gggttgttgc ggtcacgcgc ctcgatcgag aactgcccct
     7081 gcgaggcctg gcggatcttg ccgaggcggc tgcccgagtc ggcggcgaac ttcgcggcga
     7141 cctcgcgcgc cttcatggtc gcctcctcga tcatgccggg cttgacctcg ttcaggcgcg
     7201 tgaacaggta ctcgggctgg ttcgcgtact cgccgccagc gaaggcgata ccctgcttgc
     7261 cgagctgcgc gagcttgtcc atcacgccac gcacctgccc gacttcggcc gagtagacgg
     7321 tgacgatctg gtcggcggtg tagcggaact ccgcgcgctg gccgtcgccg aagcgctgcg
     7381 ccgacttgtc ggtgatcgat ggcggcgcca cgctgatcgc atcggcgccg atgccctggg
     7441 cgagcaggaa ctcgcggatg ctggcggtgt tcgcctcgat gctcgcgtac agcgactcga
     7501 ggtcgttgtc ggcggcgctg aactggatcg gccagatcac gatgtcggcc gggtattcgt
     7561 gttcggacag gcccttcacc gcgacgaaac gctcgtactc cttgaagcgg atagccgcgt
     7621 tgcccagctg gtagccgagc gccgcgaggc cgatcgcgat ggcgatgccg agcagcagcg
     7681 cgccgagcgt gttgcgagag tccatctatg cttcctccgc ggaacaggca tcgacccttc
     7741 cagtgggccg ccatccctgt gggtcgccat tcatggcgac gacag
//
LOCUS       DAORUQ010000153         7620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10827_length_7620_cov_6.035823, whole genome shotgun sequence.
ACCESSION   DAORUQ010000153 DAORUQ010000000
VERSION     DAORUQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10827_length_7620_cov_6.035823"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLW13_12280"
     CDS             <1..950
                     /locus_tag="PLW13_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71201.1"
                     /translation="GPWTLILSQHFKSAEELIATTAGMVPEGVTPTLDMFLSPVFRGY
                     FSYGSVCIRPEIEDCFYNTKFLALVRDYWKAEYAEPEGMLFNIQGPCSGAGAPHVDAT
                     RFRGMGLDNTPIWLMNTMAKSGLFKRWQARKAQVVTWYYRGSVGGGFVYWPDGPHAQP
                     RVLQAPMWGRAAVVENEMMFHTAQTNGPAAMRKPAGLALNSVMEADPEVAGGWRISTD
                     GTVIQQIPEQEFRFLVHWGANVYMDLEELKVTRDHTDDLTHEMAFDILISDLRARGEQ
                     FEMPTDPMTDKAFIGLLTRVYDVGLPLHFPPEPDEAQAA"
     gene            1085..1879
                     /locus_tag="PLW13_12285"
     CDS             1085..1879
                     /locus_tag="PLW13_12285"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71202.1"
                     /translation="MSERRIAGRAALVTGAGRGIGLAMARRFAAEGAGVLIAEFDDAK
                     GEAAAAAIRDAGGNALFRHCDVTRKDEVEAAIAACVEHFGGIDILVNNAYRGPGLARI
                     ENKSDEVFDQGLAINFWAGKWSMMAAFPHMRRKQWGRIINICSLNGVNAHMGTAEYNI
                     GKEALRTLTRSAAREWAQYGICANVICPGAATSSYKQFRAMQPEMADAIAAANPMRRM
                     GDPDRDIAGVALFLASDDACYLTGNTLYVDGGGHINGVAWAPELPD"
     gene            1885..2679
                     /locus_tag="PLW13_12290"
     CDS             1885..2679
                     /locus_tag="PLW13_12290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71203.1"
                     /translation="MSDKRVAVVTGARRGIGKAAALALAAAGFRVAVTSSNPGLPVAG
                     ARPAAGLSSTVDAITSAEGEALALPMDLLDRASIDRVVDTVIAQWGHIDVLVNNAIYQ
                     GDKLQRTVMDSDPDMLQRVFLGQVVNTTCLTQRVIAAALDRHPVTVINVGSAGGRFDD
                     AQPHPVGQAGTSFVYAASKAAFHKLAPALHTELAHAGVTAFTLNPGVVQTEALAEFVG
                     SVAGASPVEMPARVIAWLATAPEAATLSGTYQDAVTLHARYCCTGS"
     gene            2805..3356
                     /locus_tag="PLW13_12295"
     CDS             2805..3356
                     /locus_tag="PLW13_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQY71204.1"
                     /translation="MDAIALFVRAVDALNDRIGRAAAWLTLGCVLTCFAVVLLRYVFG
                     LGYPWMQELYVWQHAVVFMAGAGYTFLHGGHVNVDVLYGRLDARRRAWIDILGTLFLL
                     FPWLGVLMLTSTHFVLSSWSIREASGTANGMPGVFLLKSLLWVFCALVFAQGLALIAR
                     RGLFLAGRPLHEHDEEPVTDERI"
     gene            3381..4934
                     /locus_tag="PLW13_12300"
     CDS             3381..4934
                     /locus_tag="PLW13_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQY71205.1"
                     /translation="MSTVLVGELLSVAMFLATIVAVMAGYPVAFTLAGVGLMFATLGL
                     AFDVFDQALLSALAGRYFGTMTNETLVAVPLFVFMGVMLERSKIAEALLTTMGELFGS
                     LRGGLGYSVVIVGALLAASTGIVGATVVTMGLLSLPAMMRAGYDPKLASGTICAAGTL
                     GQIIPPSTVLIFIGDILQGANQQAQLALGNMAPEPISVGQLFAGAMLPGLLLVGLYLA
                     WIVFKAVTAPASCPPLAMSAEHRRGLGRRVLGALAPPLVLILAVLGSILAGIATPTES
                     ASVGAVGAMLLAAAKRQLNLAMLREVMRSTLTINSMVFVILLGASVFSLVFRGLGGEN
                     LVHEALAALPGGATTAVLATMLVIFLLGFFLDTFEIIFIVVPIAAPALIKLGVDPIWL
                     GVMIGMNLQTSFLTPPFGFALFYLRGVAAKFVATLDIYRGALPFVGLQLLGLALLWVL
                     PGLATELPARLFASPEPAALLAPASSGLPQAPAGSPVADDFSDLLEPPSSGRNPPWMD
                     DFRDLLSPP"
     gene            complement(5003..6103)
                     /locus_tag="PLW13_12305"
     CDS             complement(5003..6103)
                     /locus_tag="PLW13_12305"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HQY71206.1"
                     /translation="MKRRSLLKAAAVGGGAVVGAPAIAQERREWRMVTSWPKGLPGLG
                     AGAERLAANITAMSGGRLTVRVYGAGELVPALECFSAVANGTAQMGHDGAYYHTGKSE
                     GSAFFTAFPFGFTADEMQAWVMYGNGQQLWDELYAPFGIRGFLAGSTGTQMLGWFKRE
                     IRSLSDLEGLKFRAPGNQGRVLQKLGVTPVSLAGGDIYPALQSGAIDGAEWIGPYNDL
                     ALGFYQVCKYYYAHGYHEPGAALQLMINEAAWRTLTSELQSIVKAACAHAYTDMLAEF
                     NARSGPALRTLVQKHGVIVRALPEEVLLACGEKSNEVLNEIYAADRSPNRIVQRIIED
                     FLAFREQVMPWTRIGEQAFLNARRLPFEFRLR"
     gene            6386..>7620
                     /locus_tag="PLW13_12310"
     CDS             6386..>7620
                     /locus_tag="PLW13_12310"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY71207.1"
                     /translation="MSTARLPITDTQGLRQALAEANLPTLLMVYVQYTQDEAYLERFA
                     PCIRSMFSIEPTNIPEELAADLRERLFELLTRADPPQEKPLSREFVRRMMSVNVGEEV
                     AAEQVPVLYDQMAFEKPVPRKELPGRKQPPADFKVAVIGAGMGGIAAGVKLGEAGYEF
                     TIIEKNADFGGTWLENVYPGVAVDTPSHFYSYSFEQNPDWSSYYPKGAEIERYLLGVA
                     EKYGLRDKVLFNTRVLGCAWDEAKQLWRVSTSSGGREQVLEFNAIINGHGVLNRWSMP
                     DIPGLKDFKGPAMHSAGWDPSVDLRGKRVALIGTGASGAQLGTATAPVAAHLTVFERS
                     KHWVMHNPELRHDVTEGIRFALREIPHFKEWFRFRVYWFSGDGLYGNVLGDPDWPHQD
                     VSISAQNHGAREYALGYMR"
BASE COUNT         1201 a         2613 c         2585 g         1221 t
ORIGIN      
        1 acggcccgtg gacgctgatc ctctcgcagc acttcaagtc cgccgaggaa ctgatcgcca
       61 ccacggccgg catggtgccc gagggcgtga cgccgacgct ggacatgttc ctcagcccgg
      121 tgttccgcgg ctacttcagt tacggcagcg tgtgcatccg gcccgagatc gaggactgct
      181 tctacaacac gaaattcctc gcgctggtgc gcgactactg gaaggccgaa tacgccgaac
      241 ccgagggcat gctgttcaac atccaggggc cctgctccgg cgccggtgcg ccgcacgtcg
      301 atgccacgcg ctttcgtggc atgggcctcg acaacacgcc gatctggctg atgaacacga
      361 tggccaaatc gggcctgttc aagcgctggc aggcgcgcaa ggcccaggtc gtgacctggt
      421 actaccgcgg cagcgtgggc ggcggcttcg tgtactggcc ggacggcccg catgcgcagc
      481 cgcgcgtgct ccaggccccg atgtggggcc gcgccgcggt ggtcgagaac gagatgatgt
      541 tccacaccgc gcagaccaac ggcccggccg cgatgcgcaa gcccgccggc ctcgcgctga
      601 actccgtgat ggaggcagac ccggaggtag cgggtggctg gcgcatcagc accgacggca
      661 cggtgatcca gcagatcccg gaacaggagt tccgcttcct ggtgcactgg ggcgccaacg
      721 tctacatgga cctcgaggaa ctgaaggtca cgcgcgacca caccgacgac ctcacgcacg
      781 agatggcctt cgacatcctg atcagtgacc tgcgcgcgcg cggcgagcag ttcgagatgc
      841 ccaccgaccc gatgacggac aaggccttca tcgggttgct gacgcgcgtc tacgacgtgg
      901 gcctgccgct gcatttcccg ccggagccgg acgaagcgca ggccgcctga cgcaaacgac
      961 ctgatccgga tccggatacc cgcgatgagg cctgccgcga acagtccggc aggcgtctcg
     1021 acgcgcgtgc ccgttcgtac ggaatgcacg ctgcgctgaa caactgaatg aaggagtcgc
     1081 gggaatgagc gaaagaagaa ttgccgggcg tgccgcgctg gtcaccggcg cgggccgcgg
     1141 catcgggctg gcgatggcgc ggcgattcgc ggccgagggc gcgggcgtgc tgatcgccga
     1201 attcgacgac gcgaagggcg aggcggcggc cgcggcgatc cgggacgcgg gcgggaacgc
     1261 gctgttccgg cattgcgacg tcacgcgcaa ggacgaggtc gaggcagcaa ttgcggcttg
     1321 cgtcgagcac ttcggcggaa tcgacatcct cgtcaacaac gcctaccggg gccctggcct
     1381 ggcgaggatc gagaacaagt ccgacgaggt gttcgaccag ggactggcga tcaatttctg
     1441 ggccggcaag tggtcgatga tggcggcgtt cccgcacatg cgccgcaagc aatgggggcg
     1501 aatcatcaat atctgctcgc tgaacggcgt gaacgcccac atgggtacgg cggaatacaa
     1561 catcggcaag gaggccctgc gcacgctgac gcgcagtgcc gcgcgcgaat gggcgcagta
     1621 cggcatctgc gccaacgtga tctgccccgg cgcagccacc tcgtcctaca agcagttcag
     1681 ggccatgcag ccggaaatgg ccgatgccat cgccgcggcg aacccgatgc gccggatggg
     1741 cgacccggac cgcgacatcg ccggcgtggc cttgttcctg gccagcgacg atgcctgcta
     1801 cctgaccggc aacacgctgt acgtcgatgg tggcggccac atcaacgggg tggcatgggc
     1861 gcccgaactg ccggattgaa tgcgatgagc gacaagcgcg tcgcggtggt gaccggcgcc
     1921 aggagaggca tcggcaaggc ggcggcgctg gcgctcgccg cagccggttt ccgcgtcgcg
     1981 gttacgagca gcaatccggg tttgcccgtc gctggagcgc ggcccgcagc cggcctgtcg
     2041 tccaccgtcg atgccatcac gagcgcggaa ggagaggcgc tggcgctgcc gatggacctg
     2101 ctcgatcgcg ccagcatcga ccgcgtggtc gacaccgtga tcgcacagtg gggccacatc
     2161 gacgtgctcg tgaacaacgc gatctaccag ggcgacaagc tgcagcgcac cgtgatggac
     2221 agcgaccccg acatgctgca gcgcgtgttc ctcgggcagg tggtgaacac cacctgtctc
     2281 acgcagcgcg tcatcgctgc ggcgctcgac cggcaccccg tcacggtgat caacgtaggg
     2341 tccgcgggcg gtcgcttcga tgacgcacaa ccgcacccgg tgggccaggc cggcacctcc
     2401 ttcgtctacg cggccagcaa ggccgcgttc cacaagctgg cgcccgcgct gcacacggag
     2461 ctcgcgcacg caggcgtgac cgccttcacc ctgaatcccg gcgtggtgca aaccgaagcg
     2521 ctcgcagaat tcgtcggctc cgtcgcgggt gcaagcccgg tggagatgcc cgcgcgcgtg
     2581 atcgcctggc tggccaccgc acccgaggct gcgacgctgt caggcaccta ccaggacgcc
     2641 gtcacattgc acgcgcgcta ctgctgtacc ggcagctgag cagggcagga accgggagag
     2701 gcgcaggcgg cctgatccgc gcaggtccgg ccgcgcgccg ggctatcgcc agcagacccg
     2761 aatcttcgta ccatccgcgc ctgcacaacg gggtggaaat gcgggtggat gcgatagcgc
     2821 tcttcgtcag ggccgtcgac gcgctgaacg accggatcgg tcgcgcggcc gcgtggctga
     2881 cgctcggctg cgtgctcacc tgcttcgcgg tggtgctgct gcgctacgtc ttcggcctcg
     2941 gctacccgtg gatgcaggag ctctacgtgt ggcagcacgc ggtcgtgttc atggccggcg
     3001 cgggctacac cttcctgcac ggcgggcacg tcaacgtcga cgtgctctac gggcgcctcg
     3061 acgcgcgccg ccgcgcgtgg atcgacatcc tcggcaccct gttcctgctc ttcccctggc
     3121 ttggcgtgct gatgttgacc tccacgcact tcgtgctctc gtcctggagc atccgcgagg
     3181 cttccggcac cgccaacggc atgcccggcg tgttcctgct gaaatcgctg ctgtgggtgt
     3241 tctgcgcact ggtcttcgcc caggggctgg cgctgatcgc gcggcgcggc ctgttcctgg
     3301 ccgggcgccc gctccacgag cacgacgagg agcccgtcac cgatgagcgc atctgagcca
     3361 cgcgtacggc agggcaatca atgagcaccg tcctggtcgg cgaactgctg tcggtcgcga
     3421 tgttcctcgc cacgatcgtg gcggtgatgg ccggctaccc ggtggccttc acgctggccg
     3481 gcgtcgggct gatgttcgcg acgctggggc tggccttcga cgtcttcgac caggcgctgc
     3541 tctcggcact cgccggccgc tacttcggca ccatgaccaa cgagacgctg gtggccgtgc
     3601 cgctgttcgt gttcatgggc gtgatgctgg agcgctcgaa gatcgccgag gccctgctca
     3661 ccacgatggg cgagctgttc ggaagcctgc gcggcgggct cggctattcg gtggtgatcg
     3721 tcggcgccct gctcgccgcc tccaccggca tcgtgggcgc cacggtggtc acgatggggc
     3781 tgctctcgct gccggcgatg atgcgcgcgg gctacgaccc caagctcgcc tcgggcacga
     3841 tctgcgccgc cggcacgctg gggcagatca tcccgccctc gaccgtgctg atcttcatcg
     3901 gcgacatcct gcagggcgcg aaccagcagg cgcagctcgc gctcggcaac atggcgccgg
     3961 aaccgatctc ggtgggacag ctcttcgccg gcgcgatgct gcccggcctg ctgctggtgg
     4021 gcctgtacct cgcgtggatc gtgttcaagg ccgtcacggc gcccgcatcc tgcccgccgc
     4081 tcgccatgag cgcggagcat cgtcgcggac tgggccgtcg cgtgctgggc gcgctggcgc
     4141 cgccgctggt gctgatcctc gccgtgctgg gctcgatact cgcgggcatc gccacgccga
     4201 ccgagtcggc ctcggtgggc gccgttggcg cgatgctgct ggcggccgcg aaacgccagc
     4261 tgaacctggc gatgctgcgc gaggtcatgc gcagcacgct cacgatcaac tcgatggtct
     4321 tcgtgatcct gctcggcgcc agcgtgttca gcctggtgtt ccgcggcctc ggcggcgaga
     4381 acctggtgca cgaggcgctg gcggcgctgc ccggcggcgc caccacggcc gtgctcgcca
     4441 ccatgctggt gatcttcctg ctcggtttct tcctcgacac cttcgagatc atcttcatcg
     4501 tggtgccgat cgcggcgccg gcgctgatca agctgggcgt ggacccgatc tggctcggcg
     4561 tgatgatcgg gatgaacctg cagaccagct tcctgacgcc acccttcggc ttcgcgctgt
     4621 tctacctgcg cggcgtggcg gcaaaattcg tcgccacgct cgacatctac cgcggggcac
     4681 tgcccttcgt cgggttgcag ctgctgggac tggcgctgct gtgggtactg ccggggctgg
     4741 ccaccgagtt gcccgcacgc ctgttcgcga gccccgagcc cgcagcgctg ctcgcgcccg
     4801 cgtcgagcgg cctgccgcag gcgcccgccg gctcgccggt ggccgacgac ttcagcgacc
     4861 tgctggaacc gccctcctcg ggccgcaacc caccgtggat ggacgacttt cgcgacctgc
     4921 tgtcgccacc gtagctgcca cccggggaac cgtgcacgaa ctgcccgggg tcgccgcagg
     4981 cgggcggttc gtgcacggtt tatcagcgaa gacggaactc gaagggcagc cggcgcgcgt
     5041 tcaggaaggc ctgctcgccg atgcgcgtcc acggcatcac ctgttcgcgg aaggccagga
     5101 agtcctcgat gatgcgctgc acgatgcggt tcggggaacg gtctgccgcg tagatttcgt
     5161 tcagcacctc gttggacttc tcgccgcagg cgagcagcac ctcctcgggc agcgcgcgca
     5221 cgatcacgcc atgcttctgg accagggtgc gcagcgccgg acccgagcgc gcgttgaact
     5281 cggcgagcat gtcggtgtag gcgtgcgcgc aggccgcctt cacgatggac tgcagctcgg
     5341 acgtcagcgt gcgccacgcc gcctcgttga tcatcagctg cagcgcggcg ccgggctcgt
     5401 ggtagccgtg cgcgtagtag tacttgcaga cctggtagaa accgagcgcc aggtcgttgt
     5461 aggggccgat ccactcggcg ccgtcgatcg cgcccgactg cagcgccggg tagatgtcgc
     5521 cgcccgcgag cgagaccggc gtgaccccga gtttctgcag cacgcggccc tggttgccgg
     5581 gcgcgcggaa cttcagcccc tccaggtcgc tcagcgaacg gatctcgcgt ttgaaccagc
     5641 cgagcatctg cgtaccggtg ctcccggcca ggaagccgcg aatgccgaag ggcgcgtaga
     5701 gctcgtccca gagctgctgg ccgttgccgt acatgaccca ggcctgcatc tcgtcggccg
     5761 tgaagccgaa gggaaatgcc gtgaagaagg cagaaccctc gctcttgccc gtgtggtagt
     5821 aggcaccgtc gtggcccatc tgcgccgtgc cgttcgccac cgcgctgaag cactcgagcg
     5881 ccggcaccag ttcaccggcg ccgtacacgc gcacggtgag ccgcccgccg ctcatggcgg
     5941 tgatgttcgc cgcgagccgc tcggcgccgg cgccgagtcc cggcaggccc ttgggccagc
     6001 tcgtgaccat gcgccactcc cggcgctcct gcgcgatcgc cggcgcgccc accacggcgc
     6061 caccgcccac ggcggcggcc ttcaagaggg aacggcgttt cacgcgctca cacgcccgtg
     6121 gcgggtcgcg ccatcttcgg gttgagtcct gtcgagtcgt agagcttcat cgcggttcct
     6181 cgttgtcgcc ggtggcgcgc cgccgggccc gtgcggcgcc gaggatggta ggaacaatgc
     6241 cccgtccggg caagcggcgc caccacccct cgcgaaaaat tgacctcgcg caatgccgat
     6301 gccctcgacg cagattaact tacacctaag tttttatgcc ataatgcgcc accgatcctc
     6361 gcgcaacagc cagccggagc acgccatgag cacagccaga cttcccatca ccgacacgca
     6421 gggcctgcga caggcgctcg cggaagccaa tctgcccaca ctgctgatgg tctacgtcca
     6481 gtacacgcag gacgaggcct atctcgagcg cttcgcgccg tgcatccgct cgatgttctc
     6541 gatcgagccg accaatattc ccgaggagct cgcggcggat ctgcgcgagc gcctgttcga
     6601 gttgctcacg cgtgccgatc cgccgcagga aaagccgctg tcgcgcgagt tcgtgcgccg
     6661 catgatgagc gtcaacgtgg gcgaggaagt cgccgcggag caggtgccgg tactctacga
     6721 ccagatggcg ttcgagaagc cggtgccgcg caaggaactg ccgggccgca agcaaccgcc
     6781 cgccgacttc aaggtggccg tgatcggcgc cggcatgggc ggcatcgccg cgggggtcaa
     6841 gctcggcgag gcgggctacg agttcacgat catcgagaag aatgccgatt tcggcggcac
     6901 ctggctggag aacgtctatc ccggcgtggc cgtcgacacg cccagccatt tctactccta
     6961 ctccttcgag cagaatcccg actggagcag ctattacccc aagggcgcgg agatcgagcg
     7021 ctacctgctc ggcgtcgcgg agaagtacgg actgcgcgac aaggtgctgt tcaacacgcg
     7081 cgtgctcggc tgcgcctggg acgaagcaaa gcagctgtgg cgcgtcagca cgagttccgg
     7141 cggcagggaa caggtactgg agttcaatgc catcatcaac ggccacgggg tgctgaaccg
     7201 ctggtcgatg ccggacatcc cggggctgaa ggatttcaag ggtcccgcga tgcactccgc
     7261 aggctgggat ccctccgtgg acctgcgcgg caagcgcgtg gcgctgatcg gcaccggcgc
     7321 cagcggcgcg cagctcggca ccgccacggc acccgtggcc gcgcacctga cggtattcga
     7381 gcgctcgaag cactgggtga tgcacaaccc cgaactgcgg cacgatgtca ccgagggcat
     7441 ccgcttcgcg ctgcgcgaga tcccgcattt caaggaatgg ttccgcttcc gcgtgtactg
     7501 gttctccggc gacggtctct acggcaacgt gctgggcgac ccggactggc cgcaccagga
     7561 cgtctcgatc tcggcgcaga accacggcgc gcgcgagtac gcgctgggct acatgcgcga
//
LOCUS       DAORUQ010000154         7598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_10858_length_7598_cov_5.009810, whole genome shotgun sequence.
ACCESSION   DAORUQ010000154 DAORUQ010000000
VERSION     DAORUQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_7598_cov_5.009810"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLW13_12315"
     CDS             complement(<1..1454)
                     /locus_tag="PLW13_12315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQY71208.1"
                     /translation="MFEKILIANRGEIACRVIRTARRLGIHTVAVYSDADRDALHVSM
                     ADEAVHIGAAAPRESYLCGERIIAAARATGAQAIHPGYGFLSENAAFAQLCADNGLVF
                     IGPPPEAIAAMGSKSAAKRIMENAAVPLVPGYHGENQDDALLLAEAGLIGYPVLLKAA
                     AGGGGKGMRRVDSAAEFPEALAAARRESDKAFGDSHMLVEKCLIRPRHVEIQVFCDNH
                     GNGVYLFERDCSLQRRHQKVVEEAPAPGMTPAVRSAMGHAALRAAEAIGYRGAGTVEF
                     LLSAGGEFYFMEMNTRLQVEHPVTEMITRQDLVEWQLRVAAGEPLPRRQEELAVHGHA
                     LEVRIYAEDPRNDFLPVTGRLAWLQPPQESEHVRVDTGVVQGDDVSIHYDPMIAKLVV
                     WDETRPRALARLKRALAEYRIGGLTTNIAFLHNLVAHPAFAAEEVHTAFIEQHADAIF
                     RDAGHSRARQLPLAALYLLLARERQVAERANRSA"
     gene            complement(1530..3137)
                     /locus_tag="PLW13_12320"
     CDS             complement(1530..3137)
                     /locus_tag="PLW13_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY71209.1"
                     /translation="MNVIQSALNTRSQEFADNRAAMQAQVQDLRGKLAAARGGGGEKA
                     RALHTSRGKLLPRERIDALLDPGAPFLEFSALAAMGVYDEDVPAAGIVTGIGRVSGQE
                     CVIVANDATVKGGTYYPLTVKKHLRAQTIAAENNLPCIYLVDSGGANLPRQDEVFPDR
                     EHFGRIFFNQANMSARNIAQIAVVMGSCTAGGAYVPAMADEAIIVRQQGTIFLGGPPL
                     VRAATGEIVSAEDLGGADVHCRTSGVADHYARNDHHALELARRAVARLNRVKNLTLDV
                     APVREPRYAADEIYGVIPRDTRKPYDVREVIARIVDASEFDEFKSLYGTTLVCGFARI
                     WGYPVGIVANNGILFSESAQKGAHFVELCAQRGIPLVFLQNITGFMVGRQYETGGIAK
                     HGAKMVTAVACAAVPKFTVLIGGSFGAGNYGMCGRAYDPRLLFMWPNARVSVMGGEQA
                     AGVLAQIKRDQKERQGETWSAEEEAGFKRPIVERYEEQGHPYFASARLWDDGVIDPAE
                     TRMVLGLGISASLNAPIEPTRFGVFRM"
     gene            complement(3157..4320)
                     /locus_tag="PLW13_12325"
     CDS             complement(3157..4320)
                     /locus_tag="PLW13_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HQY71210.1"
                     /translation="MSWPALNFDLGADIDMLRDTVAAFCAAEIAPRAAAIDRENNFPA
                     DLWRKFGELGLLGMTVAEQYGGSGMGYLAHAVAMEEVSRASAAVGLSYGAHSNLCVNQ
                     IHRNGSEAQKSRYLPQLVSGEHVGALAMSEPNAGSDVVSMRLRAEKRGDRYVLNGNKM
                     WITNGPDASVYVIYARTDPAAGSRGITAFIVERDFPGFSRAQKLDKLGMRGSNTCELV
                     FEDCEVPAANVLGRENGGVAVLMSGLDYERVVLSGGPVGIMQACLDVVVPYMHERKQF
                     GQSIGEFQLVQGKIADMYAALNASRAYLYAVAKACDRNETARKDAAAVILYTAEAATK
                     AALDAIQILGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFNETR"
     gene            complement(4323..4781)
                     /locus_tag="PLW13_12330"
     CDS             complement(4323..4781)
                     /locus_tag="PLW13_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQY71211.1"
                     /translation="MQKSYTIRELAQEFGITARTMRHYEELGLLAPARRGQARVYSAA
                     DRTRLKLIVRGKRLGLSLEESREIIAMYDPEHGNEQQLRRLLERVREQRVALQARLAD
                     LQAMLAELDAVEAGCLASLADVDADAEADAEAEADAEAAPTRPARRRANR"
     gene            5119..6273
                     /locus_tag="PLW13_12335"
     CDS             5119..6273
                     /locus_tag="PLW13_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71212.1"
                     /translation="MSAAPPTDWNPASPEVLNDQIAAYDAMRRRCAIAHSDYLWWSLF
                     RHADLMRVLHDPASFSNAASNHLSIPNAMDPPEHTPYRQIIERYFSVERMADFEPTCR
                     AIVDALIAALPRGEPFEAMAGFAQPFALRIQCAFMGWPDRLHEPLRQWIADNHRATRA
                     GDRSAMARIALEFDGYIKQQLVQRRDAGAAAADDVTSRLLGERIHGRALSDEEIVSIV
                     RNWTVGELGTIAASVGILLHYLATHPALQDELRRRPGELPAAIDEILRIHPPLIANRR
                     VTTCPVEIGGRRLDAGERLTLIWAAANRDEAVFGDPDAFDPARNAGNNLLYGAGVHVC
                     PGAPLARLELRVLMQALLAGTHAIGLTAESAPQRASFPGSGFAVLPLRLQ"
     gene            complement(6284..6892)
                     /locus_tag="PLW13_12340"
     CDS             complement(6284..6892)
                     /locus_tag="PLW13_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQY71213.1"
                     /translation="MDAQPMRRWQPAPWLHGHVHHSGHQRIGATRVLVDPARSCARRR
                     GREPGVRHRSVHRTGLRALNMNAPQSLDAAIVEHIAEAVVFANRDGVIERWNAAAAAM
                     FGFSAAEATGQRLDLMIPEHLRAAHWRGFDAAMISGTLRLNGHATLTRGLHKSGRKLY
                     VEMSFALVRDGAGAVVGSVAVARDVTERVEREKAARAAAPPG"
     gene            complement(6900..>7598)
                     /gene="hda"
                     /locus_tag="PLW13_12345"
     CDS             complement(6900..>7598)
                     /gene="hda"
                     /locus_tag="PLW13_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HQY71214.1"
                     /translation="LMPTQLPLPVRLPDEASFANFLVCAQNRAAVEALHHFLAVGQGS
                     LFLHGPGGSGRTHLLQAACHAVEARGGAIVYLPLGELQDAPPAELLAGLEAHALVCLD
                     DADAVAGRRDWEEAMFHLYNRCLETGCRLLLAAALPPQQAGLALADLRSRLQGGAIGA
                     LAEPDDEGKIAALVLRAHNRGITLEPGVARFVWQRSPRGMAALIAVLDRLDTASLGAG
                     RRLSVPFVKEVMGW"
BASE COUNT         1126 a         2710 c         2582 g         1180 t
ORIGIN      
        1 tcggcgctgc ggttcgcgcg ctcggcgacc tggcgctcgc gcgccagcag caggtagagc
       61 gccgccaacg gcagctggcg cgcgcgcgag tgaccggcat cgcggaatat cgcatcggcg
      121 tgctgctcga tgaacgcggt gtgcacctcc tccgcggcaa aggcagggtg cgcgacgagg
      181 ttgtgcagga aggcgatgtt ggtcgtgagc ccgccgatgc ggtactcggc gagcgcgcgc
      241 ttgaggcgcg ccagcgcgcg cgggcgcgtc tcgtcccaca cgaccagctt ggcaatcatc
      301 ggatcgtaat ggatgctgac atcgtcaccc tgcaccacgc cggtgtccac gcgcacgtgc
      361 tcgctctcct gcggtggctg cagccaggcc agccgcccgg tgaccggcag gaaatcgttg
      421 cgcggatcct cggcgtagat gcgcacctcg agcgcgtgcc cgtgcaccgc gagctcctcc
      481 tggcgccgcg gcagcggctc cccggccgcc acgcgcagct gccattccac gaggtcctgg
      541 cgcgtgatca tctccgtgac cgggtgctcc acctgcagcc gcgtgttcat ctccatgaaa
      601 tagaactcgc cacccgcgct gagcaggaac tccacggtgc ccgcaccgcg gtaaccgatc
      661 gcctcggccg cgcgcagcgc cgcgtgtccc atcgcgctgc gcaccgccgg cgtcatgccg
      721 ggcgccggcg cctcctccac gactttctgg tggcgccgct gcagcgagca gtcgcgctcg
      781 aacaggtaga cgccgttgcc atggttgtcg cagaagacct ggatctcgac gtggcgcgga
      841 cggatcaggc atttctcgac cagcatgtgg ctgtcgccga aggccttgtc ggattcgcgc
      901 cgcgccgcgg ccagcgcctc gggaaattcg gccgcgctgt cgacgcgccg catgcccttg
      961 ccgccgccgc cggccgcggc cttgagcagc accggatagc cgatgagccc ggcctcggcg
     1021 agcagcagcg cgtcgtcctg gttctcgccg tggtaacccg gcaccagggg cacagcggcg
     1081 ttttccatga tgcgcttggc cgcgctcttg gagcccatcg ccgcgatcgc ctccggcggc
     1141 gggccgatga acaccaggcc gttgtcggcg cacagctgcg cgaaagcggc attctcggac
     1201 aggaagccgt agccgggatg gatggcctgg gcgcccgtgg cgcgcgcggc cgcgatgatg
     1261 cgctcgccgc agaggtagga ctcgcgcggc gccgcggcac cgatgtgcac cgcctcgtcg
     1321 gccatgctga catgcagtgc atcgcggtcg gcatcggagt acaccgccac ggtgtggatg
     1381 ccgaggcggc gcgcggtgcg gatcacgcgg caggcgattt cgccgcggtt ggcgatcagg
     1441 atcttctcga acattttcac ccacctcgtg ggtccggctt cagccggacg tcttttcgga
     1501 acgattgcga tgttcggctg aagccgaacc tacatgcgga acacgccgaa acgcgtgggc
     1561 tcgatcggcg cgttcagcga cgccgaaata cccagcccca gcaccatgcg cgtctcggcg
     1621 gggtcgatca cgccgtcgtc ccacaaccgc gccgaggcga agtagggatg gccctgttcc
     1681 tcgtaacgct cgacgatcgg gcgcttgaac ccggcctcct cctcggcgct ccaggtctcg
     1741 ccctggcgtt ccttctggtc gcgcttgatc tgcgccagca cgccggcggc ctgctctccg
     1801 cccatcaccg agacgcgcgc gttgggccac atgaacagca ggcgcgggtc gtaggcgcgc
     1861 ccgcacatgc cgtagttgcc ggcaccgaag gagccgccga tcagcacggt gaacttgggc
     1921 accgcggcgc acgcgaccgc ggtcaccatc ttcgcgccgt gcttggcgat gccgccggtc
     1981 tcgtactggc gccccaccat gaagccggtg atgttctgca ggaacaccag cggaatgccg
     2041 cgctgcgcgc acaactcgac gaagtgcgcg cccttctgcg ccgactcgct gaacaggatg
     2101 ccgttgttgg cgacgatgcc caccgggtag ccccagatgc gcgcgaagcc gcagaccagc
     2161 gtggtgccgt agagcgactt gaactcgtcg aattccgagg cgtccacgat gcgcgcgatc
     2221 acctcgcgca cgtcgtaggg cttgcgtgtg tcgcgcggaa tcacaccgta tatctcgtcc
     2281 gcggcgtagc gcggctcgcg cacgggcgcc acatcgagcg tgaggttctt cacgcggttc
     2341 aggcgcgcca cggcacggcg tgccaactcc agcgcgtgat ggtcgttgcg cgcatagtgg
     2401 tccgcgaccc cggaggtgcg gcagtgcacg tcggcgccac ccaggtcctc ggcgctcacg
     2461 atctcgccgg tcgccgcgcg caccagcggc ggaccgccga ggaagatcgt gccctgctgg
     2521 cgcacgatga tcgcctcgtc ggccatcgcc ggcacgtagg ccccgcccgc ggtgcaggag
     2581 cccatcacca ccgcgatctg cgcgatattg cgcgccgaca tattggcctg gttgaagaag
     2641 atgcgcccga agtgctcgcg gtccgggaac acctcgtcct ggcgcggcag gttcgcaccg
     2701 cccgaatcca ccaggtagat gcacggcagg ttgttctccg cggcgatggt ctgcgcccgc
     2761 aggtgctttt tcacggtgag cggatagtag gtgccgccct tgacggtcgc gtcgttggcg
     2821 acgatcacgc actcctgccc gctgacgcgc ccgatgccgg tgacgatgcc cgccgccggc
     2881 acgtcctcgt cgtagacgcc catcgcggcg agcgcggaga actcgaggaa gggcgcgccg
     2941 ggatcgagca gcgcgtcgat gcgctcgcgc ggcaacagct tgccgcggct ggtgtgcagc
     3001 gcgcgcgcct tctcgccgcc gccgccgcgc gccgcggcca gcttcccgcg caggtcctgc
     3061 acctgcgcct gcatggccgc gcggttgtcg gcgaactcct ggctgcgcgt gttgagcgcg
     3121 ctctggatca cgttcatgac tggtccctgt gcccgctcag cgggtttcgt tgaacaattc
     3181 gcgcccgatc agcatgcggc gtatctcgga ggtgcccgcg ccgatctcgt agagcttggc
     3241 gtcacgcagc agccgcccgg tcgggtagtc gttgatgtag ccgttgccgc cgaggatctg
     3301 gatcgcatcg agcgccgcct tcgtcgccgc ctcggccgtg tacaggatca ccgcggcggc
     3361 gtccttgcgc gcggtctcgt tgcgatcaca ggccttcgcc acggcataga ggtaggcgcg
     3421 actggcgttg agcgcggcgt acatgtcggc gatcttgccc tggaccagct ggaactcgcc
     3481 gatggactgg ccgaactgct tgcgctcgtg catgtaggga acgaccacgt cgagacaggc
     3541 ctgcatgatc cccacggggc cgcccgagag caccacgcgc tcgtagtcca gccccgacat
     3601 cagtaccgcc acgccgccgt tctcgcgccc gagcacgttc gcggccggca cctcgcagtc
     3661 ctcgaacacc agctcgcagg tgttggagcc gcgcatgccg agcttgtcga gcttctgcgc
     3721 gcgcgagaag ccggggaaat cgcgctcgac gatgaaggcc gtgataccgc gcgagccggc
     3781 cgcgggatcg gtcctggcgt agatcacgta gacgctggcg tcgggcccgt tggtgatcca
     3841 catcttgttg ccgttgagca cgtagcggtc gccgcgcttc tcggcgcgca ggcgcatgct
     3901 gaccacgtcg gaaccggcgt tcggctcgct catcgccagc gcgccgacgt gctcgccgct
     3961 caccagttgc ggcagatagc ggctcttctg cgcctcgctg ccattgcggt ggatctggtt
     4021 cacgcagaga ttggagtgcg cgccgtagga gagccccacc gccgccgagg cgcggctcac
     4081 ctcttccatc gcgacggcgt gcgccaggta gcccatgccg gagccgccgt actgctcggc
     4141 gacggtcatg ccgagcagcc cgagctcgcc gaacttgcgc cacaagtcgg cgggaaaatt
     4201 gttttcgcga tcgatcgccg cggcgcgcgg cgcgatctcg gcggcgcaga aggccgcgac
     4261 ggtatcgcgc agcatgtcga tatccgcgcc gagatcgaag ttcaacgcgg gccaggaaat
     4321 gctcatcggt tcgccctccg gcgtgcgggt cgggtgggtg cggcttcggc gtcggcttcg
     4381 gcttcggcgt cggcttcggc gtcggcgtcg acgtcggcga gcgaggcaag gcagccggcc
     4441 tcgacggcgt cgagctcggc cagcatcgcc tgcagatccg ccagccgcgc ctgcagcgcc
     4501 acgcgctgct cgcgcacgcg ttcgagcaga cgccgcaact gctgttcgtt gccgtgctcc
     4561 ggatcgtaca tcgcgatgat ctcgcggctc tcctcgaggc tgagcccgag gcgcttgcca
     4621 cgcacgatca gcttgagacg ggtgcggtcg gccgccgagt agacgcgcgc ctgtcctcgg
     4681 cgcgccggcg ccagcaggcc gagctcctcg taatggcgca tggtgcgcgc ggtgatgccg
     4741 aactcctgcg cgagctcgcg gatcgtgtag ctcttctgca tcgcagcgtc cccggatggt
     4801 ggcgcggaaa cgatacctgc cgtttacgtt aacgtcaact tcgtgcgcca tccggggcac
     4861 gcaggccgcc attcatggcg acaggcgcgg ccgacgaacc ctctgtcggc atgaatgccg
     4921 acctagatgg tcgtaaccag cccatcaccg atttcacaaa gggaacactg agccgctgaa
     4981 aggccgcgtg ccgcgcagca agccccattc gctcgcaagt tgcgcccgcg caaacgctgc
     5041 agcgggcagc gttgcgccga ggcccgcgct gcagcgatga tgtccgccat gtcagccccg
     5101 aagacagcga ggacatcgat gagcgctgcc ccgccgaccg actggaaccc ggcgtcgccg
     5161 gaagtgctga acgaccagat cgccgcctac gatgcgatgc gccggcgctg cgcgatcgcg
     5221 catagcgact acctgtggtg gtcgctgttc cggcatgccg atctgatgcg cgtgctgcac
     5281 gacccggcga gcttcagcaa tgccgcgtcg aaccatctgt cgattccgaa tgcgatggac
     5341 ccgccggaac acacgccgta ccggcagatc atcgagcgct atttcagcgt cgagcgcatg
     5401 gccgacttcg agcccacgtg ccgggcgatc gtcgatgcgt tgatcgcggc cctgccgcgc
     5461 ggcgaaccct tcgaggcgat ggccggcttc gcccagccgt tcgcgctgcg catccagtgc
     5521 gcgttcatgg gctggcccga ccgcctgcac gagccgctgc gccagtggat cgccgacaac
     5581 catcgcgcca cgcgcgccgg cgaccgctcg gcaatggcgc ggatcgcgct cgagttcgac
     5641 ggctacatca agcagcaact ggtgcagcgg cgcgatgcgg gcgcagcagc ggccgacgac
     5701 gtgaccagca ggctgctggg cgagcgcatc cacggccgcg cgctgagtga cgaggaaatc
     5761 gtcagcatcg tgcgcaactg gacggtcggc gaactgggta cgatcgccgc gagcgtcggc
     5821 atcctgctgc actacctggc cacgcatccg gcgctgcagg acgagctgcg ccgacggcct
     5881 ggcgagctgc cggcggccat cgacgagatc ctgcgcatcc acccgccgct gattgccaac
     5941 cgccgcgtca ccacctgccc ggtcgagatc ggtggccgcc ggctcgacgc cggcgagcgc
     6001 ctgacgctga tctgggcggc agccaaccgc gacgaagcgg tgttcggcga ccccgatgcc
     6061 ttcgaccccg cgcgcaacgc cggcaacaac ctgctgtatg gcgccggcgt gcatgtctgc
     6121 cccggggcac cgttggcccg gctcgaactg cgcgtgctga tgcaagcgct gctggcgggc
     6181 acgcatgcaa tcgggctcac cgccgagtcc gccccgcaac gcgcgagctt cccgggcagc
     6241 ggttttgcgg tgttgccact ccgcctgcag taacaggcga ccgtcagccc ggcggcgcgg
     6301 cggcgcgcgc cgccttctcg cgctcgaccc gctcggtgac gtcgcgcgcc accgcgaccg
     6361 aaccgacgac cgcgcccgcg ccgtcgcgca cgagcgcgaa gctcatctcg acatagagct
     6421 tgcgccccga cttgtgcaac ccgcgggtca gcgtcgcgtg gccgttcagg cgcagcgtgc
     6481 cgctgatcat cgccgcgtcg aagccgcgcc agtgggcggc gcgcaggtgc tcggggatca
     6541 tcaggtccag ccgctggccg gtggcctctg cggcgctgaa accgaacatt gctgccgctg
     6601 cggcgttcca gcgttcgatc acgccgtcgc ggttggcgaa gaccaccgcc tcggcgatgt
     6661 gctcgacgat cgctgcgtcc agggattgcg gtgcgttcat gttcagggct ctcaatccag
     6721 ttcggtgtac agatcgatgt cgaacgccgg gttctcgacc acgccgccgg gcgcatgacc
     6781 gcgcggggtc gaccagaacg cgtgtcgcgc cgatgcgctg atggccgctg tggtgcacgt
     6841 ggccgtgcaa ccacggcgcc ggctgccagc ggcgcatcgg ctgcgcgtcc acacggccct
     6901 caccagccca tcacttcctt cacgaaggga acactgagcc gccgcccggc gcccagcgat
     6961 gcggtatcca gacgatccag cacggcgatc agcgccgcca tgccgcgcgg actgcgctgc
     7021 cagacgaagc gcgccacccc gggctccagc gtgatgccgc gattgtgcgc gcgcagcacc
     7081 agcgcggcga tcttgccctc gtcatcgggc tcggccagcg cgccgatcgc cccgccctgc
     7141 aggcgcgagc gcaggtccgc gagcgcgagc ccggcctgct gcggcggcag cgccgcggcc
     7201 agcagcaggc ggcagccggt ttccaggcag cggttgtaga gatggaacat cgcctcttcc
     7261 cagtcgcgtc ggcccgcgac cgcgtccgcg tcgtcgaggc agaccagtgc gtgcgcctcg
     7321 aggcccgcga gcaactccgc cggtggcgca tcctgcaact cgccgagcgg caggtagacg
     7381 atcgcacccc cgcgcgcctc gaccgcgtgg caggctgcct gcagcaggtg ggtgcgcccg
     7441 ctgccgcccg gaccgtgcag gaacaggctg ccttggccga ccgcgaggaa atggtgcaat
     7501 gcctcgaccg ccgcgcgatt ctgcgcgcag accaggaagt tcgcgaacga ggcctcgtcg
     7561 gggaggcgca ccggcagcgg caattgcgtg ggcatcag
//
LOCUS       DAORUQ010000155         7451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_11185_length_7451_cov_6.311655, whole genome shotgun sequence.
ACCESSION   DAORUQ010000155 DAORUQ010000000
VERSION     DAORUQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11185_length_7451_cov_6.311655"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLW13_12350"
     CDS             <1..785
                     /locus_tag="PLW13_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQY71215.1"
                     /translation="EEFREVAMDTAQAAAYRDLSGRLTAELKQALAKRDTTLLGVVLN
                     VLLAWSDCCFRSETVVHPRTRQTLAFVPAQFNELEVMPKERELIEICKQEKAEGRKTL
                     VYSVYTGTRDTTSRLKVLLEQEGFRVAVLRASVDASRREDWIAEQLDRGIDVLITNPE
                     LVKTGLDLLEFPTIVFMQSGYNVYSLQQAARRSWRIGQKQAVRVIYLGYAASSQMTCL
                     GLMAKKIMVSQSTSGDVPESGLDVLNQDGDSVEVALARQLVH"
     gene            complement(819..1526)
                     /locus_tag="PLW13_12355"
     CDS             complement(819..1526)
                     /locus_tag="PLW13_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQY71216.1"
                     /translation="MLDYAHPSIAGLVAARGWSMLPVFQRIGAVYDYVRDEVAFGYNV
                     SDDLPASRVLAEGIGQCNTKGTLLMALLRAVGVPCRFHGFTIDKQLQKGAVTGLWYLL
                     APRSIIHSWVEVFFEGRWVNLEGFILDKPYLSSLQRRFAHQSGSFCGYGAATPDLQNP
                     AVDWKGCDTYIQREGINRDYGVFNSPEAFYARHGTNLKGFKRWLYANVVRHLMNRNVE
                     NIRRGVLSRGREAVHQK"
     gene            complement(1608..4130)
                     /locus_tag="PLW13_12360"
     CDS             complement(1608..4130)
                     /locus_tag="PLW13_12360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQY71217.1"
                     /translation="MSAADSYPSAGLSSEQAARLLAQEGPNELPRPPKRTVWRILREV
                     VREPMFQLLAAAVTIYLVLGDVSEAGVLLAFLVVIVAITLVQERRTERVLEALHDMTS
                     PRALVWRDGQRQRIAGTELVVGDRIELAEGDRVPADARLLEANDLAVDESLLSGESLP
                     VLKSADHDPSAHAAVVQVHAGTMVVAGQAVACVTATGARSEIGRIGKTLGAIETQATP
                     LHRQTRQLVRWFSALGLVVSVAVGLLFAWTRGDWLGGALAGITMAMSMLPQEFLLILT
                     VFMAMGAWRLSRHRVLTRRAATIEALGAATVLCTDKTGTLTQNKMAIEALMRFGPEGR
                     TAWRARDGDPGEAFDELRRYGVLASEREPVDPMERAFHQSAQRRPGHVPADWELVHEY
                     SLSPQWPVMSHVWRMPGSDRHVVAAKGAPETVALLCGLPDDERDQMLAEAGTLADQGM
                     RVLAVAGAAWSGTEWPDTQQGFEFRLLGLVGFADPLRDDVPQAVEDCRRAGIRVVMIT
                     GDHPGTALAIARQAGIDGRGGALRGHEVALLDEPALRRSVATTTVFARVAPEQKLRIV
                     QALKANGEVVAMTGDGVNDAPSLKAAHIGIAMGGRGTDVAREASSLVLLDDDFATLVR
                     AIRLGRRIYDNLRKAMRFVFAVHVPIAGLTLVPLMFGWPLLFTPMHIAFLEIVIDPVC
                     SIAFEAEDEERDVMQRPPRDPGAPLFSLSMIARSLLQGTVVLTLVGLLFWLLLRLGWS
                     DEAARTSAFIALVASNIALILANRSFSAGVGQIMRLHNRALWGMVLATLALLVIVIGI
                     PLLRHLFGFALPDLPSVAAATGVAVAVLLLLLLQKRLEAAWG"
     gene            complement(4135..4716)
                     /locus_tag="PLW13_12365"
     CDS             complement(4135..4716)
                     /locus_tag="PLW13_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQY71218.1"
                     /translation="MMESLLVSTGVVALAEIGDKTQLLAFILAARFKKPFPIILGILA
                     ATTVNHGLAGALGAWITANVSPEILRWVLGLSFIGMAVWTLIPDKIEEEETQVARKFG
                     VFGATLVTFFLAEMGDKTQIATVAMAAHYGAPLIVIIGTTLGMLIADVPAVFVGDKLA
                     AKIPMKLVHSIAAAIFALLGLATLFGAGAKLGL"
     regulatory      complement(4717..4895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            5058..5660
                     /locus_tag="PLW13_12370"
     CDS             5058..5660
                     /locus_tag="PLW13_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HQY71219.1"
                     /translation="MQPLTCVAHRLAVSAVLAGCVLVTGCAATGTASNSTVHDSLPEP
                     AVLVRTIAPEPEPSLIPVARYGRYTLVEMVPEPAQRDLLRQVIEISIPRTLDASVGDA
                     LRHVLLRTGYRLCDAPDAASLFTLPLPAAHLRLGPLPLRDALLVLAGSAWELSVDDAS
                     RSVCFTRAAASRSASASPAPATPSSSAPVAEPAQLEESQP"
     gene            5657..6301
                     /locus_tag="PLW13_12375"
     CDS             5657..6301
                     /locus_tag="PLW13_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71220.1"
                     /translation="MIVPQLQSENAGRARWLKIAAAAWLLLVSALAVVNSVGLSRLTE
                     QSQASAQDAHVQAINTRVAELEQQAAASKNQPKPVTQPDFEAARKTLDERLAQVEQAQ
                     AGDTHAGDFQALQARVGDIEARMKRTAAPVAIPRRAAEPAKPKVPEPPFNVVGVDLRG
                     GERFLSVAAPRASSVLDVWLLREGDAVGAWHLLAIEARAAVFSVDGQTQRIALP"
     gene            6311..7033
                     /locus_tag="PLW13_12380"
     CDS             6311..7033
                     /locus_tag="PLW13_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HQY71221.1"
                     /translation="MKRAAICFAGILVLVASAARAQSAPVASSRAVPAQVQSSNDAAL
                     DERLARDWGLRPEEWARYRQLMQGPLGIYSPNLDPLTALGIEARSDEERNRYAELQVQ
                     AESKRVGKTLAYQRAYDAAWKRLYPGQQRVSMPGAKAPGAGNRGSGRLAVFVKAECPP
                     CEQRVRQLQAAGTAFDLYMVGSRQEDARIRQWATQAGIDAGRVRSRTITLNHDAGRWL
                     SLGLPGDLPAVVREVNGQWQRQ"
     gene            7018..>7451
                     /locus_tag="PLW13_12385"
     CDS             7018..>7451
                     /locus_tag="PLW13_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQY71222.1"
                     /translation="MAAAVAATTLVHRACCTALVLGLCASAAFAFGQEVPPPAYQLAA
                     QQAGVPSPVLFAVALQESGARLRGRLIPWPWTLNVAGRAERYATRAEACAGIRRALAR
                     TPANRVDVGLGQVNLGYHAHRYEHPCDLLDPYRNLAIAAEIL"
BASE COUNT         1254 a         2568 c         2461 g         1168 t
ORIGIN      
        1 acgaggagtt ccgcgaggtg gcgatggaca cggcgcaagc cgcggcctat cgcgatctgt
       61 ctggccggct gaccgcggag ctcaagcagg ctctggcgaa gcgggacacg acgctgctcg
      121 gtgtggtcct caacgtgctg ctggcctggt cggattgctg cttccggtcg gagacggtag
      181 tgcatccgcg cacgcgccag accttggcct tcgtcccggc tcagttcaac gagctggagg
      241 tgatgccgaa ggagcgcgag ctgatcgaga tctgcaagca ggagaaggcg gaaggtcgca
      301 agacgctggt ctattcggtc tacaccggca cgcgcgacac cacgtcgcgc ctgaaggtgc
      361 tgctggagca ggaaggtttt cgagtggcgg tgctgcgcgc aagcgtggac gcctcccgcc
      421 gcgaagactg gatcgccgag cagttggatc gtgggatcga cgtgctcatc accaacccgg
      481 agctggtcaa gacgggcctc gatctgttgg agtttccgac gatcgtgttc atgcagtcgg
      541 gctacaacgt ctacagcctg cagcaggccg caagacgctc atggcgtatc ggccagaagc
      601 aggccgtgcg cgtgatctat ctcggctacg ccgcttcctc gcagatgacc tgcctgggac
      661 tgatggccaa gaagatcatg gtctcgcaaa gcacgtcggg agacgtaccc gaatcgggac
      721 tggatgtgct gaatcaggac ggtgattccg tcgaggtggc actggcccgg caattggtgc
      781 attgatccca catatcttgg cggcccctcg gggccgcttt atttttggtg aacggcttcg
      841 cgccctcggc tcagaactcc tcgtcgaatg ttctcgacgt ttcggttcat caagtgcctg
      901 acgacgttcg cgtacagcca gcgcttaaag cccttcaagt tcgttccgtg tctggcgtag
      961 aacgcctccg gcgaattgaa aactccgtag tcccggttga ttccctctcg ctggatatag
     1021 gtgtcgcaac ctttccagtc caccgccggg ttctgcaagt ccggcgtcgc ggctccatat
     1081 ccgcagaagg aacccgactg atgggcgaag cgcctctgga gcgaagacag ataaggcttg
     1141 tcgaggatga acccttccag gtttacccaa cggccttcga agaagacttc tacccaactg
     1201 tggatgatgc tgcgcggtgc aaggagatac cacagaccag tgacagcacc tttctgaagc
     1261 tgcttgtcta tggtgaagcc atggaatcga catggcacgc ccactgcacg cagcagggcc
     1321 atcaacaagg tgcctttcgt gttgcattgg ccgatacctt cggccagaac tcgtgaagcc
     1381 ggcaagtcgt cggacacgtt gtagccgaag gccacctcgt ctctcacgta atcgtagaca
     1441 gcgccaatgc gctggaatac cggcagcatc gaccagccac gtgcggcaac aagcccggca
     1501 atgctgggat gggcgtagtc gagcaaggct gtgggctcgg tcaagtcgct tgtaggactg
     1561 ttatcaacca tggctttagg tgaaacacaa tgcattgtcg gtagtgccta gccccaggcc
     1621 gcctccaggc gcttctgcaa cagcaacagc agcagaaccg cgaccgccac ccctgttgcc
     1681 gcggccacgg agggcaggtc tggcagcgca aagccgaaca gatgccggag cagcgggatg
     1741 ccgatcacga tgaccagaag ggccagcgtc gcaagcacca tcccccacaa cgctcgattg
     1801 tgcaaccgca tgatctgtcc cacaccggcg ctgaacgagc gattggccag gatcagcgca
     1861 atgttcgagg ccaccagcgc gatgaaggcg gaggtcctcg ccgcttcgtc ggaccacccg
     1921 agccgcagca gcaaccagaa cagcagaccg accagcgtca gcaccacggt accctgtagc
     1981 aggctccgcg cgatcatgga cagcgagaac agcggcgcgc cgggatcgcg cggcggccgc
     2041 tgcattacat cgcgttcttc gtcttccgcc tcgaaggcga tcgagcagac gggatcgatc
     2101 acgatttcga ggaacgcgat gtgcatcggc gtgaacagca gcggccagcc gaacatcagg
     2161 gggaccagcg tcagcccggc gatcgggacg tgcaccgcga agacgaagcg catcgccttg
     2221 cgcaggttgt cgtagatgcg ccgacccagc cggatcgccc gcaccagcgt tgcgaaatcg
     2281 tcgtccagca gcacgagcga cgatgcctcg cgcgcgacgt ccgtgccgcg cccgcccatc
     2341 gcgatgccga tgtgagcagc cttgagcgag ggcgcgtcgt tgacgccgtc accggtcatc
     2401 gcgaccactt cgccgttggc cttcagggcc tggacgatgc gcagcttctg ctcgggcgca
     2461 acccgcgcga agaccgtcgt cgtcgccacg ctgcgccgca gggccggctc gtccagcaat
     2521 gcgacctcgt ggccccgaag cgcgccgccc cggccgtcga tgcccgcctg gcgggcgatg
     2581 gccagcgccg tccccgggtg gtcgccggtg atcatcacca cgcggatgcc cgcgcgccgg
     2641 caatcctcga ccgcctgcgg cacgtcgtcc cgcagcggat cggcgaaccc caccagtccg
     2701 agcaaccgga attcgaagcc ttgctgggtg tcgggccact cggtgccgga ccaggctgca
     2761 cccgccaccg ccagcacgcg catgccctgg tcggccagcg tgcctgcctc ggccagcatc
     2821 tggtcgcgct cgtcgtcggg caggccgcac agcaacgcca cggtctccgg cgctcccttg
     2881 gccgccacga catgccgatc gctgccaggc attcgccaga catgggacat cacgggccat
     2941 tgcgggctca ggctgtactc gtgcaccagc tcccaatcgg ctggaacatg cccggggcga
     3001 cgttgcgccg attggtggaa cgcacgctcc atcggatcga cgggctcgcg ctcgctggcc
     3061 agcacgccgt agcgccgcag ctcgtcgaac gcctcgcccg gatcgccgtc gcgggcgcgc
     3121 caggccgtcc tgccttcggg accgaagcgc atcagcgcct cgatggccat cttgttctgg
     3181 gtcagggtgc ccgtcttgtc ggtgcacagc accgtggccg cacccagcgc ttcgatggtg
     3241 gccgcgcggc gcgtcagcac gcgatgccgc gacagccgcc acgcgcccat ggccatgaag
     3301 accgtgagga tcagcaggaa ctcctgcggc agcatggaca tcgccatggt gatgccggcc
     3361 agcgcgccgc ccagccagtc gccgcgggtc caggcgaaca gcaggccgac ggccacgctc
     3421 accacgaggc cgagcgcgga gaaccagcgc acgagctgac gggtctgccg gtgcagcggc
     3481 gtggcctgcg tctcgatggc cccgagcgtc ttgccgatgc ggccgatctc gctgcgcgcg
     3541 ccggtggccg tcacgcatgc caccgcctgt ccggcgacca ccatcgtgcc ggcatggacc
     3601 tggaccacgg cagcatgcgc ggaagggtcg tggtcggccg acttgagcac cggcagcgat
     3661 tcgccgctca agagggattc gtcgacggcc aggtcgttgg cctccagcag ccgggcatcg
     3721 gccggcacgc ggtcgccctc ggccagctcg atccggtcgc cgaccacgag ttcggtgccc
     3781 gcgatccgtt gtcgctgccc gtcccgccag accagcgcgc gcgggctggt catgtcgtgc
     3841 agcgcttcca atacccgctc ggtgcgccgc tcctgcacca gggtgatggc gacgatcacg
     3901 accaggaagg ccagcagcac gccggcttcc gagacgtcgc ccagcacgag gtagatggtc
     3961 acggccgccg ccaggagctg gaacatcggc tcgcgcacca cctcgcgcag gatgcgccac
     4021 accgtccgct tcggcggccg cggcagttcg ttcggcccct cctgtgcgag caagcgggcc
     4081 gcctgctcgg aactgagccc cgcggacgga tagctgtcgg ctgcgctcat ggtgctagag
     4141 gcccagtttc gcgccggcgc cgaacagggt agcgagacct agcagcgcga agatggcggc
     4201 ggcgatggag tgcaccagct tcatcggaat cttggcggcg agcttgtcgc cgacgaacac
     4261 cgccggcaca tcggcgatca acatcccgag cgtggtgccg atgatcacga tcagcggcgc
     4321 gccgtagtgc gcggccatgg ccaccgttgc gatctgcgtc ttgtcgccca tctcggccag
     4381 gaagaaggtg acgagggttg cgccgaagac gccgaacttc cgggcgacct gggtttcctc
     4441 ttcctcgatc ttgtcgggga tcagcgtcca gaccgccatc ccgatgaagg acaggcccag
     4501 cacccagcgc aggatctccg ggctgacgtt ggcggtgatc caggccccga gcgcgccggc
     4561 caggccgtga ttgacggtgg tcgcggccag gatccccagg atgatcggaa acggcttctt
     4621 gaagcgagcc gcgaggatga acgcgaggag ctgcgtcttg tcgccgatct cggcgagcgc
     4681 cacgaccccg gtcgaaacga ggagagattc catcagagta cccaggccgg acgcgagacg
     4741 attgaccacg acgtatcccc ggccttgtcg gagacatcgt ggtcaaaggt cttgccaagc
     4801 ttgggactgc ttacgccatg acctgcgggt caagtgtgtt gacgtaagcc tcttcgcgat
     4861 gaagagcggc tactccccaa tgacgcgcac attgtaacag gcccgtcgcg ccggatcgag
     4921 gagggagtgc cgctgcagtg ttctccgcac tggtggcgat cacgccatga atcgggtgca
     4981 ttccagttcc ctttgtttgc tgcccgagcg ctctatggcg gcgatggtga gggctccgtg
     5041 ttccagggaa tcgccccatg caaccactca cctgcgtcgc tcatcgcctg gctgtgtccg
     5101 ccgtgctggc cggctgcgtg ctcgtcaccg gctgcgccgc gacgggcacg gcgagcaact
     5161 cgactgtcca tgactcgctt cccgagcctg cggtgttggt caggaccatc gccccggagc
     5221 ccgagccgag cctgatcccg gtggcccgct acggccgcta cacgctggtc gagatggtgc
     5281 cggagccggc gcagcgcgac ctgctgcgcc aggtgatcga gatttcgatt ccgcggaccc
     5341 tggacgcaag cgtcggcgat gccctgcggc acgtgctgct gcgcaccggc taccgccttt
     5401 gcgatgcgcc ggacgccgca tcgctgttca cgctgccgct gccggcggcg catctgcggc
     5461 tcgggccgct gccgctgcgc gatgccttgc tcgtacttgc cggctcggcc tgggaactgt
     5521 ccgtcgatga cgcttcgcgc tcggtgtgct tcacgcgcgc tgccgcctcg cgctccgcga
     5581 gcgcaagccc cgccccggcc accccatcat cgagcgcgcc ggtcgccgaa cccgcccaac
     5641 tcgaggagtc acagccatga tcgtcccgca gctccagtcc gagaacgccg gccgtgcgcg
     5701 ctggttgaag atcgcagccg ccgcgtggct gttgctcgtg agcgctctgg ctgtggtcaa
     5761 cagcgtaggc ctgtcgcgcc tgaccgagca aagccaagcc agcgcccagg atgcgcacgt
     5821 gcaggcgatc aacacgcgcg tcgccgaact cgaacagcaa gcggcggcct cgaagaacca
     5881 gcccaagccc gtcacccaac ccgacttcga ggccgcccgc aagacgctgg acgaacgcct
     5941 ggcgcaggtt gagcaggccc aggcgggaga cacccatgcg ggtgacttcc aagcgctgca
     6001 ggcgcgcgtg ggcgacatcg aagcgcgcat gaagagaacc gccgcgcctg tcgcgatacc
     6061 ccggcgtgcc gccgagccgg ccaagcccaa ggtgccagag cctccgttca acgtcgtggg
     6121 cgtggacttg cgcggcggcg agcgcttcct gtcggtggcg gcacctaggg cctcgtcggt
     6181 gctggacgtt tggctgctgc gcgaaggcga tgccgtgggc gcttggcatt tgctggcgat
     6241 cgaggcgcgc gccgccgtgt ttagcgtgga cggccagacc cagcgcatcg cgctgccgta
     6301 ggagccggcc atgaagcgcg ccgcaatctg cttcgccggc atcctcgttc tcgtcgcgag
     6361 cgcggcccgg gcacagtcgg cgccggtcgc ctcctcgcgc gcggtgcccg cccaggtcca
     6421 gagcagcaac gatgccgcgc tggacgaacg cctggcacgc gattggggcc tgcggcccga
     6481 ggagtgggcg cgctaccgcc aactgatgca gggaccgctg ggcatctact ccccaaacct
     6541 cgatccgctc acggcgctgg gcatcgaggc gcgcagcgac gaggaacgca accgctatgc
     6601 ggagctgcag gtgcaggcgg aatcgaagcg cgtgggcaag acgctggcct accagcgggc
     6661 ctacgacgcg gcctggaagc ggctctatcc ggggcagcag cgcgtgagca tgccaggcgc
     6721 gaaggcgccg ggcgccggca accggggctc gggccggctg gctgtcttcg tgaaagccga
     6781 gtgcccgccg tgcgaacagc gcgtgcgcca gttgcaggcg gccggcaccg ccttcgacct
     6841 ctacatggtc ggcagccgcc aggaagacgc ccgcatccgc cagtgggcga cgcaggccgg
     6901 catcgacgcc ggccgcgtgc gctcgcgcac catcacgctc aaccatgacg ccgggcgctg
     6961 gctgtcgctc ggcctgcccg gcgatctgcc cgccgtggtg cgcgaggtca acggccaatg
     7021 gcagcggcag tagcggcaac aacgttggtg caccgcgcct gctgcacggc cctggtcctc
     7081 ggcctctgcg ccagcgcagc cttcgccttc ggccaagagg tgccaccgcc cgcctaccag
     7141 ctcgccgcac agcaggcggg cgtgccgtcc ccggtgctgt tcgcggtagc gctgcaggag
     7201 agcggcgcac ggctgcgcgg ccgcctgatc ccctggccgt ggacgctcaa cgtcgccggc
     7261 cgggccgaac gctacgccac acgtgcggaa gcctgtgcag gcatccgtcg ggcgctcgcc
     7321 cgcacgccgg ccaaccgcgt cgacgtcggc ctcggtcagg tcaacctcgg ctaccacgcg
     7381 caccgctacg agcatccctg cgatctgctc gacccatatc gcaacctcgc catcgccgcg
     7441 gaaatcctgc a
//
LOCUS       DAORUQ010000156         7352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_11419_length_7352_cov_6.829519, whole genome shotgun sequence.
ACCESSION   DAORUQ010000156 DAORUQ010000000
VERSION     DAORUQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11419_length_7352_cov_6.829519"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..246)
                     /locus_tag="PLW13_12390"
     CDS             complement(28..246)
                     /locus_tag="PLW13_12390"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HQY71223.1"
                     /translation="MSEAIRLDQMSTAEKLSTMEAIWDDLCRHADDVSSPEWHEGVLA
                     EREVRYRSGAESAEDWATAKESIRRQIP"
     gene            677..1930
                     /locus_tag="PLW13_12395"
     CDS             677..1930
                     /locus_tag="PLW13_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY71224.1"
                     /translation="MAQVDRESYPHWDRLRRLPAPAGLTNHEWWLATKFARQLSYRET
                     PLTDREGKPFVIGKPDALLQLLPRLDNQLGGKIGLQELTVNQDTRSRYVISSLMEEAV
                     TSSQLEGAATTRADAIAMLRTGRKPRDRSERMILNNYLAMQRIGQLRNEPLTPDLVLE
                     LHAVVTEDTLDEAHSAGRMQTPTDLRVKVWDEQTGEILHDPPPADQLPDRLRLMCDFA
                     NGTVEAQGYLHPIVKAILLHFWLAHDHPFADGNGRTARALFYWHMLREGYWLTEFISI
                     SSLIRKAPVKYARSFLWTETDANDLTYFVLAQLGIIAQSIDALENYIARKSSELRSVD
                     QRLRALDRINHRQKALLAHALKNRHAVYSIQSHCRSHGVAYATGRADLLELCRLGLLT
                     QLRQGKAFKFRAPDDLSSRLMLLEE"
     gene            complement(1959..2951)
                     /locus_tag="PLW13_12400"
     CDS             complement(1959..2951)
                     /locus_tag="PLW13_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71225.1"
                     /translation="MTALETALTGLSLGTPVTHANLTLFPLLGGDAGESAYLTLGEAL
                     AAGQARITEVSEQGSVPELRFVNEATRPVLLLDGEELVGAKQNRIVNLTILVPANSEL
                     VIPVSCVEAGRWRHESASFMAAERAHFARGRAQKMADVSHSLASRGERRSDQGAVWEE
                     IRMKAGRMQAESSTAAAAAMYEAHAHRLEDFVQAMPPAPGQCGAAFAVNGALLGVDLF
                     DASATYAKQAKKLLTSYALDAIDESRHEAPAARAEDCQALLADVLRADCRAFKAVGLG
                     EDLRLSGAGVKGAALELDGRVVHLCAFREQSRQAQRGEGAAERMVRASRRRNLH"
     gene            complement(3045..3365)
                     /locus_tag="PLW13_12405"
     CDS             complement(3045..3365)
                     /locus_tag="PLW13_12405"
                     /inference="COORDINATES: protein motif:HMM:NF027074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HQY71226.1"
                     /translation="MSSKSPAHSKRAAIPRASDFSRTFRKDWDRLSRSGRFDMRRLKE
                     AMLLLIANDGPLGAEWRDHPLKGNWADHRECHIGGDFLLIYQADSTQIVFVRAGTHAD
                     LFNE"
     gene            complement(3337..3618)
                     /locus_tag="PLW13_12410"
     CDS             complement(3337..3618)
                     /locus_tag="PLW13_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HQY71227.1"
                     /translation="MAAHTSMLHVRVDDDVKEQATEALAAMGLTVSDAVRLFLRRVVV
                     EQAFPLELKVPNAETRAAMDESRALMAARRARFSTPEALIGELEKPGTQ"
     gene            complement(3743..4033)
                     /locus_tag="PLW13_12415"
     CDS             complement(3743..4033)
                     /locus_tag="PLW13_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQY71228.1"
                     /translation="MSISPGDVVTLSQARAQLSDLADQAKAGAEKIITKNGESYVALI
                     DAARLDYYHGLERERIHLLLIDEAARALDDVSAGRTRPARAALQAMKRRRGR"
     gene            complement(4112..5035)
                     /locus_tag="PLW13_12420"
     CDS             complement(4112..5035)
                     /locus_tag="PLW13_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY71229.1"
                     /translation="MRYTNRDGIQRIAGVGELLRWQLGLHEEKRARTPATGVPVPFVA
                     NDGALLRRATKDALTWIGHASFLVQLGGSSVLIDPVLSPSLGGVVPRNVPPGLDWNAL
                     PRRIDAVLVTHNHRDHMDAPTLKRLGPDPVYLVPEGLGGWFRRAGLKRVVEMRWWQTE
                     RIAGLDISFVPAQHWSRRGIGDTNASWWGGFVIERGGIRLYHSGDTAWFDGFAEIGER
                     CGEIHAAMLPIGAYAPRWFMRHQHMNPEDAVNAFSALGAARFVAMHWGTFKLTDEDLR
                     EPPQLLRAIWREQGCKPSQLEIPAIGQTLIF"
     gene            5183..6160
                     /locus_tag="PLW13_12425"
     CDS             5183..6160
                     /locus_tag="PLW13_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HQY71230.1"
                     /translation="MDRTERFYKINQQLDNGHCVSFEKLREACEVSRATLQRDLEYMR
                     TRLNAPILYDRERNGYCLSRETPQVGAQYELPGLWFNSQEIHALLTMQHLLANLDPGG
                     LLAQHIEPLQLRLNTLLGAAEDAVSDIRHRVLVVGTGKRTLKLAHFERVGSALLRRKR
                     LQINYYARSTGETTAREVSPLRLVHYRENWYLDAWCHMRNDLRNFAVDSILDVDVLER
                     RARDIARRTVESVLGPGYGIFAGNAVATAQLRFTPERARWVGAESWHPHQRGEYEPDG
                     SYLLRVPYADDRELIMDILRYGADCEVLGPPALRKAVARELRKAATRYA"
     gene            complement(6232..>7352)
                     /locus_tag="PLW13_12430"
     CDS             complement(6232..>7352)
                     /locus_tag="PLW13_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQY71231.1"
                     /translation="MTMSLTVPPPIAVPAPERRSRYTACLLGGAIGDALGAPVEFMSR
                     QEILRRFGPAGIREHASAYGRTGAITDDTQMTLFTAEGLLRAAAAGHPPGHHARVAEV
                     AAAYQRWLLTQDEDPARHADAGGLLGIAALHSRRAPGTTCLAALRQVAARGSREIVAA
                     RGRSHNGEPGVDLRANNNSKGCGGVMRVAPAGLLLAGLIADEAQLAREAFALGSDLAA
                     LTHGHPSGCLPAGVLAAVIALLVREVPLRDALARTKPVLATRAGCEETLAAITAAERL
                     ASQGAANHDTLAQLGGGWVGEEALAISLYCALAAPDLESAVVLAVNHAGDSDSTGAIT
                     GNLCGAALGMDALPARWLEGLELDRTTRELALALCAVS"
BASE COUNT         1167 a         2519 c         2486 g         1180 t
ORIGIN      
        1 ggctggcagc gcgcaggatc ctgatcttca cgggatttga cgcctgatgc tctctttggc
       61 cgtggcccag tcttcagctg attccgcgcc gctgcggtag cggacttcgc gctcggcaag
      121 gacaccctcg tgccactcgg gagaggagac gtcgtccgca tggcggcaca ggtcgtccca
      181 gattgcctcc atcgtgctca gcttttctgc ggtgctcatc tgatcgagcc tgattgcttc
      241 gctcatgccg ccctccaagt ctggctggtc tgcggggacc acaatatgca atcggcgaat
      301 gcgagtacag cgtccggtgc ctgcgcccgc cctacgctca cttcggtccg cgccgtcgcg
      361 actcccgcac catccgcccc gcggcagtgg agaggccggc acaccgcgcc ccgggaggcc
      421 accgagtctt gattgtcggc gatcaggagc gattggatgc tttgcctcgg cgctcctgct
      481 atcaatgaac tatcaatttg tatcgaattg atagtttctt ccaagatgcc tactatcagc
      541 catctatcaa ttgctaccaa attgatactt ggcttggatg actgccatga agcggccggt
      601 acgaccccct tcgcttcccg agttgcttgg caagctcgag ccggcgcgtc tcgcggaaat
      661 attgaccctg taccccatgg cgcaggtcga ccgcgaaagc tacccgcact gggaccgcct
      721 gcgccggctg cctgcgccag cagggctcac aaatcacgaa tggtggctgg ccaccaagtt
      781 cgcccggcag ctgtcgtacc gcgaaacgcc gctgaccgac cgcgagggca aacccttcgt
      841 catcggcaag cccgacgcgc tgctgcagtt gcttccgcgg ctcgacaacc agttgggcgg
      901 gaagatcggt ctccaggaac tgaccgtcaa ccaggacacg cgctcgcgct acgtcatcag
      961 ttcgttgatg gaggaggcgg tgacgtccag ccaactcgag ggggcggcca cgacgcgagc
     1021 cgacgccatc gcgatgctgc gcaccgggcg caagccgcgc gaccggagcg agcgcatgat
     1081 cctcaacaat tacctcgcga tgcaacggat cgggcagctg cgcaacgagc cactgacgcc
     1141 ggacctggtc ctcgagttgc acgccgtcgt taccgaggac accctggacg aggcacactc
     1201 ggccggccgc atgcagacac ccacagacct gcgagtgaag gtctgggatg agcagacggg
     1261 cgagatcctt cacgatccgc cgcccgccga ccaattgccg gaccgtctcc ggctgatgtg
     1321 cgacttcgcg aacggcacgg tcgaggcgca gggctacctg caccccatcg tcaaggccat
     1381 cctcctgcat ttctggctcg cgcacgacca cccctttgcc gacggcaacg ggcgcacggc
     1441 cagggctctg ttttactggc acatgctccg cgagggttac tggctgacgg agttcatctc
     1501 gatttccagc ctgatccgca aggcacccgt gaaatacgcg cgatcattcc tgtggacgga
     1561 aacggatgcg aacgacctga cgtacttcgt gctcgcgcag ctcggtatca tcgcgcaatc
     1621 cattgacgcg ctggagaact acattgcgcg caagtcgagt gaattgagat cggtcgacca
     1681 gcggctcagg gcgctggacc gcatcaatca tcgtcagaag gcgctgttgg cgcacgcgct
     1741 gaagaaccgc catgccgtct attccatcca gtcgcattgc cgctctcacg gtgtcgccta
     1801 cgcgaccggt cgcgccgacc tgctggagct gtgccggctg ggcctgctca cacagctgcg
     1861 gcagggaaaa gccttcaagt tccgcgcgcc ggacgatctg tcctcgcgac tgatgctgtt
     1921 ggaggagtga gctcccgggc gccgtttgcc ggagtggttc agtgcagatt acgccgccgc
     1981 gacgcccgca ccatccgctc ggccgcaccc tcgccccgct gcgcctgccg ggactgctcg
     2041 cggaacgcgc acaggtgcac cacgcgcccg tccagctcca gcgccgcgcc cttcacgccg
     2101 gctccggaga ggcgcaggtc ctcgcccaga cccacggcct tgaaggcgcg gcagtcggcg
     2161 cgcagcacgt cggccagcag tgcctggcag tcttcggccc tcgcggccgg ggcctcgtgc
     2221 ctggactcgt cgatggcgtc cagcgcgtag ctcgtcagca gcttcttcgc ctgcttcgcg
     2281 taggtggcac tggcgtcgaa caggtccacg ccgagcagcg cgccgttcac cgcgaaggcg
     2341 gcgccgcact gcccgggcgc gggcggcatg gcctgcacga agtcctcgag ccggtgtgcg
     2401 tgggcctcgt acatggcggc ggcggccgcg gtggacgatt cggcctgcat gcggcccgcc
     2461 ttcatgcgga tctcctccca caccgcgccc tggtcgctgc gccgctcgcc gcggctcgcc
     2521 agcgagtggc tgacgtcggc catcttctgc gcgcggcccc tggcgaagtg ggcgcgctcg
     2581 gcggccatga agctcgccga ttcgtggcgc cagcggccgg cctcgacgca ggagaccggg
     2641 atcacgagct cgctgttggc cggcaccagg atggtgaggt tcacgatgcg gttctgcttg
     2701 gcgcccacca gttcctcgcc gtcgagcagc agcacggggc gcgtcgcctc gttgacgaag
     2761 cgcaactccg gcacgctgcc ctgctcggag acctcggtga tgcgggcctg cccggccgcc
     2821 agcgcctcgc cgagcgtgag gtaggcggac tcgcccgcat cgccgccgag cagcgggaac
     2881 agggtgaggt tggcgtgcgt gacgggggtg ccgaggctga ggccggtgag ggcggtttcg
     2941 agtgcggtca tgggcgctcc tgcggacgaa tggctatgga gcgccagtat ggggaggggg
     3001 gagcatcagg gggtgaggct ggtacggggc gcggcggcga gcgactattc gttgaagagg
     3061 tccgcgtgtg tccctgcgcg aacgaagacg atctgggtcg aatcagcctg gtagatcagc
     3121 aagaagtcgc cgccgatatg gcattcgcgg tgatctgccc aattgccctt gagcgggtga
     3181 tcccgccact cggcaccgag cggcccgtcg ttcgcgatca ggagaagcat tgcctccttc
     3241 aatcgccgca tgtcgaaccg tcctgatcgc gacaggcggt cccagtcctt gcggaatgtt
     3301 cggctgaagt ccgaggcgcg cgggattgca gcccgcttac tgtgtgccgg gcttttcgag
     3361 ctcaccgatc agcgcctcgg gagtggaaaa gcgcgctcgg cgcgccgcca tcagcgcacg
     3421 cgactcgtcc attgcggcac gtgtctcggc attggggacc ttgagctcga gcgggaaggc
     3481 ctgctcgaca acgacgcgac gcaggaacag cctgacggca tccgataccg tcagccccat
     3541 cgccgccagc gcttcggtcg cctgctcctt gacgtcatca tccacccgga catgaagcat
     3601 cgaagtgtgg gctgccatgg tgtattcctc aaaaacaggt ccattttgta tctcaaaagc
     3661 gatacggtca accgcccgtc gatcgtgaaa caaaccggca tagcgttacg cgccagtcga
     3721 tcatcaagat ctggctcgcc gatcagcgcc cccggcgccg cttcatcgcc tggagcgcgg
     3781 cccgtgccgg gcgggtcctg cccgccgaaa cgtcgtcgag cgccctggca gcctcgtcga
     3841 tgagcaacag gtggatccgc tcgcgctcga gcccgtggta gtagtcgagc cgggcggcat
     3901 cgatcagcgc gacgtagctc tcgccgttct tggtaatgat cttttcggcg ccggccttgg
     3961 cctggtcggc cagatccgag agctgggcgc gcgcctgcga cagggtgacc acgtccccgg
     4021 gcgatatcga cattcgcgac ctcccgctca ttcaatgttt ggaatagcgt acgggatatg
     4081 gccacacgct atcacccgcg atttgttcgg gtcaaaagat cagggtctga ccgatcgcgg
     4141 gaatctccag ctgcgatggc ttgcagccct gctcccgcca gatcgcgcgc agcagctgtg
     4201 gcggctcgcg caggtcctcg tcggtgagct tgaaggtgcc ccagtgcatc gcgacgaagc
     4261 gcgcggcgcc cagcgccgag aacgcattca cggcatcctc gggattcatg tgctggtggc
     4321 gcatgaacca gcgcggcgcg taggcgccga tcggcagcat cgcggcgtgg atctcgccgc
     4381 agcgctcgcc aatctccgca aagccgtcga accacgcggt atcgcccgag tggtacaggc
     4441 gaatgccgcc gcgctcgatc acgaagccgc cccaccagct ggcgttggtg tcgccgatgc
     4501 cgcgccggct ccagtgctgc gccggcacga agctgatgtc gagcccggcg atgcgctccg
     4561 tctgccacca ccgcatctcc acgacgcgct tcagacccgc gcggcgaaac cagccgccca
     4621 gtccttcggg caccaggtaa accggatcgg ggccgaggcg cttcagcgtc ggcgcgtcca
     4681 tgtggtcgcg gtggttgtgc gtgaccagca ccgcgtcgat gcgccgcggc aacgcgttcc
     4741 agtcgagccc cggcggcacg ttgcgcggca ccacgccgcc cagcgagggc gacagtaccg
     4801 ggtcgatcag cacgctgctg ccgccgagct gcaccaggaa cgaggcgtgc ccgatccagg
     4861 tcagcgcgtc cttcgtcgcg cggcgcagca acgccccgtc gttcgcgacg aaaggcaccg
     4921 gcacgccggt ggcgggcgtg cgcgcgcgtt tttcctcgtg caggccgagc tgccagcgca
     4981 gcaattcgcc gacaccggcg atgcgctgga tgccgtcacg gttggtgtaa cgcatgggct
     5041 cggttcgcca tccaattcga agtagccatt gtgctcgaaa atgccggaga cgggcgatgg
     5101 cggcagaggg tctagaatgc ttgtaccctg ccggttcagg ccgttgcccg actgccgcgg
     5161 cgcaggcatg aggatcggct cgatggaccg caccgaacgc ttctacaaga tcaaccagca
     5221 gctcgacaac gggcactgcg tgagcttcga aaagctgcgc gaggcctgcg aggtctcgcg
     5281 cgccacgctg cagcgcgacc tcgagtacat gcgcacgcgg ctcaacgcgc cgatcctcta
     5341 cgaccgcgag cgcaacggct actgcctctc gcgcgaaacg ccgcaggtgg gcgcgcagta
     5401 cgagctgccg gggctgtggt tcaactcgca ggagatccac gcactgctga ccatgcagca
     5461 cctgctggcg aacctcgacc cgggcgggct gctggcgcaa cacatcgagc cgctgcagct
     5521 gcgcctgaac acgctgctcg gcgcggccga ggacgcggtg agcgacatcc gccatcgcgt
     5581 gctggtggtg ggcaccggca agcgcacgct gaagctcgcg cacttcgagc gcgtgggctc
     5641 ggcgctgctg cggcgcaagc ggctgcagat caactattac gcgcgcagca ccggcgagac
     5701 caccgcgcgc gaggtctcgc cgctgcgcct ggtgcactac cgcgagaact ggtacctcga
     5761 cgcgtggtgc catatgcgca atgacctgcg caatttcgcg gtcgattcga tcctggacgt
     5821 ggatgtgctc gagcggcgcg ccagggacat cgcgcggcgc accgtcgaga gcgtgctcgg
     5881 gcccggctac ggcatcttcg ccggcaacgc ggtcgccacg gcgcagctgc gtttcacgcc
     5941 cgagcgcgcg cgctgggtgg gcgccgagag ctggcatccg caccagcgcg gcgaatacga
     6001 gcccgacggc agctacctgc tgcgcgtgcc ctacgccgac gaccgcgagc tgatcatgga
     6061 catcctgcgc tacggcgccg actgcgaggt gctgggcccg ccggcgctgc gcaaggcggt
     6121 ggcgcgcgaa ctgcgcaagg ccgccacgcg ttacgcgtag gtccgcattc atgcggacgg
     6181 gtgcgtgccc gatgaatttt gtccggctga agccggaccc acggggagcg ctcagcttac
     6241 cgcgcacagg gccagtgcaa gctcgcgcgt ggtgcggtcc agttcgagcc cctcgagcca
     6301 gcgcgccggc agcgcgtcca tgccgagcgc ggcgccgcag aggttgccgg tgatcgcgcc
     6361 ggtcgaatcg ctgtcgccgg cgtggttcac ggcgagcacg acggcgctct cgagatcggg
     6421 cgcggcaagg gcgcagtaga gactgatcgc cagcgcctcc tcgccgaccc agccgccgcc
     6481 cagttgcgcc agcgtgtcgt ggttcgccgc accctggctc gcgaggcgtt cggccgcggt
     6541 gatggccgcg agcgtttcct cgcaacccgc ccgcgtggcg agcaccggct tcgtgcgggc
     6601 cagtgcatcg cgcagcggca cctcgcgcac cagcagggcg atcaccgcgg ccagcacgcc
     6661 ggcgggcagg cagccgctcg ggtggccgtg cgtcagcgcg gcgaggtcgg agcccagcgc
     6721 gaaggcctcg cgtgcgagct gcgcctcgtc ggcgatcagc cccgccagca gcaggccggc
     6781 gggtgccacg cgcatcacgc cgccgcagcc cttggaattg ttgttcgcac gcaggtcgac
     6841 gccgggctcg ccattgtggg agcggcccct ggccgcgacg atctcgcgcg agccgcgggc
     6901 tgcgacctgc cgcagcgctg ccaggcaggt ggtaccgggc gcgcggcgac tgtgcagcgc
     6961 ggcgatgcca agcaggcccc cggcgtcggc gtggcgggcg ggatcctcgt cctgcgtgag
     7021 cagccagcgc tggtaggcgg cggccacttc cgccacccgg gcgtggtgcc ccggcggatg
     7081 gcctgcggcg gcggcacgca gcaagccctc ggcggtgaac aacgtcatct gggtgtcatc
     7141 ggtgatcgcg ccggtgcggc cgtaggcgct ggcgtgttcg cggatgcccg ccgggccgaa
     7201 gcggcgcagg atctcctggc gcgacatgaa ttccaccggc gcgccgagcg cgtcgccgat
     7261 ggcgccgccc agcaggcagg cggtgtagcg cgagcgccgc tcgggagcag gcacggcgat
     7321 gggaggcggg acggttagcg acatggtcat gc
//
LOCUS       DAORUQ010000157         7309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_11521_length_7309_cov_7.736697, whole genome shotgun sequence.
ACCESSION   DAORUQ010000157 DAORUQ010000000
VERSION     DAORUQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11521_length_7309_cov_7.736697"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1306)
                     /gene="cfa"
                     /locus_tag="PLW13_12435"
     CDS             complement(158..1306)
                     /gene="cfa"
                     /locus_tag="PLW13_12435"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HQY71232.1"
                     /translation="MATQPHVHPAAAETAEHRALAQLLEPADVSLDGKRPWDIVVHDA
                     RMPARVLAQGSLGAGESYMDGWWDCAQLDEMLARVLRSELDRRLHPAGALKLATLAAL
                     RNPQSLRRAFIVGRAHYDIGDDLFERMLDTRMIYSCAFWDRAGDLATAQEAKLDLVCR
                     KLGLASGMRVLDIGCGWGGAAQFAAERYGVEVTGITVSKHQAEAAAARCSGLPVDIRL
                     EDYRRHSASYDRIWSIGMFEHVGVRNYRAYLRKVRELLRPDGLFLLHTIGSNTSVRST
                     DPWIDKYIFPNSMLPSVAQIARAAERWWVIEDWHGFGSHYDHTLLAWYRNFEARWPEI
                     AARYGERFRRMWRFWLLGSAASFRARRNQLWQFVLSPHGVPGGYRAVR"
     gene            complement(1378..2109)
                     /locus_tag="PLW13_12440"
     CDS             complement(1378..2109)
                     /locus_tag="PLW13_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY71233.1"
                     /translation="MTDPVTLGAALLLGLVASGHCVLMCGGITAALGIATAKRADGRP
                     QTTLLAGYQFGRIASYSLAGLIFGGALGAAVDWLDIEAVRRGLRMLAALALLVGALVA
                     LGILRDPGGGLGRRLWPKLAPLGRRLLPVKTLPRAFGFGMVWGWMPCGIVYTMLMIAG
                     LQLDALQSAATMAAFGLGTAPAMFATGFGAQRFAGFAAQPTARRVAGVALLAAASLTF
                     AGPWLATYAPGIAHWLPFDCAAMSH"
     gene            complement(2106..2318)
                     /gene="ccoS"
                     /locus_tag="PLW13_12445"
     CDS             complement(2106..2318)
                     /gene="ccoS"
                     /locus_tag="PLW13_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQY71234.1"
                     /translation="MNILLVMIPLSILLLIGAGIAFFWAVDHDQFDDLDTPRLLPLLD
                     DPRSPPQSDARDEASSDGMPSSESPP"
     gene            complement(2315..4771)
                     /locus_tag="PLW13_12450"
     CDS             complement(2315..4771)
                     /locus_tag="PLW13_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQY71235.1"
                     /translation="MTAGGCWHCGEPLPASDAPQARVAGTSHPVCCQGCRAAAEWIDQ
                     LGLADYYRLRSAPAPRAPDAAEAARTGTTWARPELERHVVRSLSGETREAIVLIEGLR
                     CTACVWLIERALAALPGVVSTQVSAAARRARIVWDATRCSLATILETLMRAGYRPLPL
                     DAAALDDARRHEQRTALKQLVVAGFGSMQAMMYASGLYFGAFDGIDTTTRDLLRWIGL
                     LVATPVVFYAARPFFAGALRTLRARRLGMDVPVAIAIALIYSASVIEAWRGGAEVYFD
                     SVSMFVFFLLTGRYLEMRARHRASDLTDALARLTPTFADRVRADGSLERIGAIELVPG
                     DRVHVADGGAVPADGALEGNRCRVDESLVSGESAPVVKERGDRLVAGSVLVDGSAQMT
                     VTRVGADTVLAGILALIARAATERPRLAAAGERAAAGFVARVLTLAALTAIGWSLVDP
                     SRALTATLAVLVVSCPCAFALAVPAALTRALGVLAQRGVLVARPDAIEGLAGATHVLF
                     DKTGTLTEPSLSLERIDTFTGVDRHQALALAAVLARGSRHPASRAISGAAASLRPNDL
                     LPAAQAQRAEPGAGLEGIVEGRRLRLGRADFALARDAIPEEFADAVVLADDAGPIAAF
                     RLAERLRADAGPTIEALAAAGLGLEIVSGDDAGKVAAMAARLRIARWRARLHPADKLA
                     RLAELRASGARVIAVGDGINDAPVLAGADVSVALASGAELAQTASDIVLTGERLGGLA
                     EARRIARQTLAILRQNQRWALVYNLSVIPLGALGFVPPWLAALGMSTSSLIVVLNALR
                     IGRGAERADSAPAMQAQAAT"
     gene            complement(4959..>5150)
                     /locus_tag="PLW13_12455"
     CDS             complement(4959..>5150)
                     /locus_tag="PLW13_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HQY71236.1"
                     /translation="LGAPRLSDSTWTWGKGDLAMIENTITNGAHGVMPAWNTRLTADD
                     TRVIIAWLRSQTRERPARL"
     assembly_gap    5151..5250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5251..5702)
                     /locus_tag="PLW13_12460"
     CDS             complement(<5251..5702)
                     /locus_tag="PLW13_12460"
                     /inference="COORDINATES: protein motif:HMM:NF026065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQY71237.1"
                     /translation="MNSFWSLWVMFLVVFNASITLFLFLWAQKVEIPVLEDGTTGHVW
                     AHGVLRESVRRLPRWWVAASAAFYIWGVGFLLLYPGFGGFKGLLGWSSHGEHALQVEV
                     NGANLADLHSQFADLSPEQLSANPVALRLGERLFVDNCAACHERDARG"
     gene            complement(5699..5854)
                     /locus_tag="PLW13_12465"
     CDS             complement(5699..5854)
                     /locus_tag="PLW13_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HQY71238.1"
                     /translation="MTAAWGQVAGIVTVVMMLLFIGIWIWVWNDGHKPKYDRLARLPM
                     ADAEDRP"
     gene            complement(5851..6480)
                     /gene="ccoO"
                     /locus_tag="PLW13_12470"
     CDS             complement(5851..6480)
                     /gene="ccoO"
                     /locus_tag="PLW13_12470"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQY71239.1"
                     /translation="MAKGYKYFEAVEKHAGKLGILTAIMVSIGGLAEITPLFVEAHAI
                     TAVEGVKPYDPLRLAGKDIYVREGCYTCHTQMIRALRAETQRYGHYSISSESVYDRPH
                     QWGSKRTGPDLARVGGKYSDEWQRLHLMDPRAVVQASNMPAYPWLAEEKLDADDISAR
                     MRALRRLGDPYSDADISGVPAALEGKTALDALVAYLQGLGVSPMVETAP"
     gene            complement(6491..>7309)
                     /locus_tag="PLW13_12475"
     CDS             complement(6491..>7309)
                     /locus_tag="PLW13_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY71240.1"
                     /translation="LGMMYYFVPRQAQQPLWSYRFSIVNFWALISVYMWAGSHHLLYT
                     ALPDWVQSVGMAFSILLLMPSWGSAANGLFTFNGAWQRVASDPAAKFMVMALVFYAAA
                     TTEGSLMAVKTVNSLSHYTDWTVAHVHSGAIGWVAMITIGSLYAMAPRALGRPAMHSK
                     RSMELHFWMHTAGLILYVAAMWTAGVTEGLMWRATDANGALIYPFIDSLIAVRPFYLI
                     RFLGGLLIVGGMIAMAWNLWHTAADARARLIKPIPVPIPEPEPLQVPAPVAARA"
BASE COUNT         1239 a         2452 c         2494 g         1024 t
ORIGIN      
        1 ccggtcacga gcgcttccat ggcgatgagt tccggttcga tgtcgttcag cacgcccgcc
       61 ggcaggcgat gcctcaggac gctgcgcagc agccagtcgt ccgaccatgc atagggcggc
      121 gtgggcggcg actgaatgaa cggcgccgtg ccagccatca gcgcaccgcc cgatagccac
      181 cgggcacgcc gtgaggcgac agcacgaatt gccacagctg attgcgccgg gcgcgaaagg
      241 aggcggccga gcccagcagc cagaagcgcc acatgcggcg gaagcgctcg ccgtagcgcg
      301 ccgcaatctc cggccagcgc gcctcgaagt tgcgatacca ggcgagcagc gtgtgatcgt
      361 agtgcgagcc gaagccgtgc cagtcctcga tgacccacca gcgctccgcc gcgcgcgcga
      421 tctgcgcgac cgagggcagc atcgagttcg gaaagatgta tttgtctatc cacgggtcgg
      481 tgctgcgcac cgaagtgttc gagccgatcg tgtgcaacag gaagagccca tccggcctca
      541 gcaactcgcg cactttgcgc aagtaagccc ggtagttgcg cacgccgacg tgttcgaaca
      601 tgccgatgga ccagatgcgg tcgtagctcg cgctgtggcg acgatagtcc tcgagccgga
      661 tatcgaccgg gagcccgctg cagcgcgcgg cggcagcctc ggcctgatgc ttcgagacgg
      721 tgatgccggt cacctcgaca ccgtagcgct cggccgcaaa ctgcgccgcg ccgccccacc
      781 cgcaaccgat gtcgagcacg cgcatgccgc ttgcgagccc gagcttgcgg cacacgagat
      841 cgagcttggc ttcctgcgcc gtggcgagat cgcccgcgcg atcccagaag gcgcagctgt
      901 agatcatgcg tgtatcgagc atgcgctcga aaagatcgtc accgatatcg tagtgcgcgc
      961 gaccgacgat gaaggcacgc cgcaggctct gcggattgcg cagtgccgcg agcgtcgcga
     1021 gcttcagggc gcccgcgggg tgcagccggc gatcgagttc gctgcgcagc acgcgggcca
     1081 gcatttcatc gagctgcgcg cagtcccacc agccgtccat gtaagactcg ccggctccga
     1141 gcgagccctg cgcgagtacc cgcgccggca tgcgcgcgtc atgcacgacg atgtcccatg
     1201 ggcgcttgcc gtcgagcgaa acatccgctg gttcaagcag ctgcgcgagc gcgcgatgtt
     1261 ccgcagtctc ggccgccgca ggatggacgt gaggctgcgt ggccatacgg ccccaacgtt
     1321 acgactgccg aggcgaaggt gcaatgatgt atgtagttgt acggtgtctg gcgggggtca
     1381 gtgcgacatc gcggcgcagt cgaacggcag ccagtgcgcg atccccggcg cgtacgttgc
     1441 gagccacggg cccgcgaagg tgagggaggc cgcagcgagc agtgcgacgc ccgcgacacg
     1501 ccgggcagtg ggctgcgcgg cgaagccggc aaatcgttgt gcgccaaagc cggttgcaaa
     1561 catcgcgggc gccgtgccga gaccgaaggc cgccatcgtt gctgccgatt gcagggcatc
     1621 gagctgcagc cctgcgatca tcagcatcgt gtagacgatg ccgcacggca tccagcccca
     1681 caccatgccg aagccgaatg cgcgcggcag tgtcttgacc ggcaggagtc gtcggccgag
     1741 aggcgcaagc ttcggccaca ggcgccgtcc gaggccgccg cctggatcgc gcaggatgcc
     1801 gagcgcgacg agggcgccga ccagcagcgc gagcgcggcg agcatgcgca gcccgcgtcg
     1861 cacggcctcg atgtcgagcc agtcgaccgc ggcgccgagc gcgccgccga aaatcaggcc
     1921 cgcaagcgag taggaagcga tgcggccaaa ctggtatcca gcgagcagcg tggtctgcgg
     1981 gcggccatcg gcgcgcttcg ccgtcgcgat gccgagcgcg gcagtgatgc cgccgcacat
     2041 cagcacgcag tggccgctcg cgacgaggcc gagcagcagc gcggcgccga gcgtaaccgg
     2101 gtcggtcatg gcggcgactc gctggagggc attccgtcgg acgatgcctc gtcgcgcgca
     2161 tcgctttgcg gtggcgaccg cggatcatcg agcagcggca gcaggcgcgg tgtgtcgaga
     2221 tcgtcgaact ggtcgtgatc gaccgcccag aaaaacgcga tgccggcgcc gatcagcagc
     2281 aggatcgaaa gcgggatcat gacgagcaga atgttcatgt ggcagcctgt gcctgcatcg
     2341 ccggcgccga gtcagcgcgc tcagcgcccc gcccgatgcg cagcgcgttc agcacgacga
     2401 tgagggagct cgtcgacatg ccgagcgccg cgagccaggg cggcacgaag ccgagcgcgc
     2461 cgagcggaat gaccgagagg ttgtagacga gcgcccagcg ctggttctgg cgcaggatcg
     2521 cgagcgtctg gcgcgcgatg cgccgcgctt cggcgaggcc gcccagccgc tcaccggtca
     2581 gcacgatatc gctggcggtc tgcgcgagct ccgcgccgct cgcaagtgcc actgatacat
     2641 cggcgccggc gaggacgggc gcatcgttga tgccatcgcc gactgcgatg acgcgtgcgc
     2701 cgctggcgcg cagctcggcg aggcgcgcga gcttgtcggc cgggtgcagc cgcgcccgcc
     2761 agcgcgcgat acgcagtcgc gcggccatcg ctgcgacctt gcccgcgtca tcgccactca
     2821 cgatttcgag cccgagcccc gcggcggcaa gtgcttcgat cgtgggcccg gcatcggcac
     2881 gcagtcgctc tgcgagccgg aatgccgcga tcggccctgc atcgtccgcg agcaccacgg
     2941 catcagcaaa ttcttcgggt attgcatcgc gcgcaagcgc gaagtcggcg cggccgaggc
     3001 gcagtcgccg gccttcgacg atgccttcga ggcccgcgcc gggttcggcc cgctgggctt
     3061 gtgcagccgg cagcaggtcg tttggccgca gcgaagccgc cgcgcccgaa atcgcccggg
     3121 aagcgggatg gcgactgccg cgcgcgagca cggcggcgag cgcaagggcc tggtgtcgat
     3181 cgacgcctgt gaacgtgtcg atgcgttcga gcgaaaggga aggctccgtg agcgttccgg
     3241 tcttgtcgaa caggacatgc gtggcgcctg cgagcccttc gatggcgtcg gggcgcgcga
     3301 ccagcacgcc gcgttgcgcc agcacgccga gcgcgcgcgt gagggcggcc ggcacggcga
     3361 gcgcgaacgc acaggggcag gagacgacga gcacggcgag cgtggcggtg agggcgcgcg
     3421 aggggtcgac gagactccag ccgatcgcgg tgagcgcagc gagcgtcagc acccgggcga
     3481 cgaagcccgc cgccgcgcgc tcgcccgctg cggcgagtcg cggccgctcc gtcgcagcgc
     3541 gcgcgatgag cgccaggatc cctgcgagca ccgtgtcggc gcccacgcgc gttaccgtca
     3601 tctgtgcgga tccatcgacg agtacgctgc ccgcgaccag cctgtcgcca cgctccttca
     3661 cgacgggcgc ggactcaccg gacaccagcg actcatcgac acgacaacga ttgccctcga
     3721 gcgcgccgtc cgcgggcacc gccccgccgt cggcgacgtg cacccgatca ccggggacga
     3781 gttcgatggc accgatgcgt tccagggatc cgtcggcacg cacccggtcg gcgaaggtcg
     3841 gcgtgaggcg ggcgagggcg tcagtgaggt cgcttgcgcg atgacgcgca cgcatttcga
     3901 gatagcggcc ggtgagcagg aagaagacga acatgctgac cgagtcgaag tacacctctg
     3961 cgccgccgcg ccacgcttcg atcacgcttg cggagtagat cagcgcgatc gcgatcgcca
     4021 cgggcacgtc catgccgagc cgtcgcgcgc gcagcgtgcg cagcgctccc gcaaaaaaag
     4081 gtcgcgcggc atagaagacg accggggtcg cgacgagcag gccgatccag cgcaacagat
     4141 cgcgtgtggt cgtgtcgatg ccgtcgaagg cgccgaagta gaggccgctg gcatacatca
     4201 tcgcctgcat cgaaccgaag cccgccacca cgagctgctt gagggcagtg cgttgttcgt
     4261 ggcgtcgcgc gtcatccagc gccgccgcgt cgagcggcag cgggcgatag ccggcgcgca
     4321 tcagcgtctc gaggatcgtc gcgagtgagc accgcgtggc atcccacacg atgcgcgcgc
     4381 ggcgagctgc ggcgctcacc tgggtactga cgacccccgg cagcgcagca agtgcgcgct
     4441 cgatcagcca cacgcacgcc gtgcagcgca agccctcgat cagcacgatc gcctcccggg
     4501 tctcgccgga cagcgatcgc acgacgtggc gctcgagttc ggggcgtgcc cacgtcgtac
     4561 ccgtgcgagc cgcttcagcg gcatcgggcg cgcgcggcgc cggtgcgctg cgcagtcggt
     4621 agtagtcggc gagcccgagc tggtcgatcc attcggcggc cgcgcgacag ccctggcaac
     4681 acacgggatg gctggtaccg gcgactcgcg cctgcggcgc gtcgctcgcc gggagcggct
     4741 cgccgcagtg ccagcagccg cccgcggtca ctgcggtgtc tcgggaggcg gggcgcgccc
     4801 cgcaggcatc ctgaggacgg tactgcctga ggtgggcagc gcctcgtcat cgtggcgcgc
     4861 cccgagtacg agcagcgcga tgcagcccgc gatcgtcgcc gcaaaaagca cggcacccag
     4921 ccacagaacg ggctggcgat accatgccgc ctgcgcgttc acagccttgc cgggcgctct
     4981 cgcgtctgcg agcgcaacca cgcgatgatc acgcgcgtgt cgtccgcggt gagccgcgtg
     5041 ttccacgcgg gcattacgcc gtgcgcgccg ttcgtgatcg tattctcgat catggcgaga
     5101 tcgcccttgc cccaggtcca ggtcgagtcg gagagccggg gcgcgcccag nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgccgcggg cgtcacgctc gtgacacgcc
     5281 gcacagttgt cgacgaacag gcgctcacct agccgcagcg ccacgggatt tgcgctgagt
     5341 tgctccggac tgaggtcggc gaactgcgag tgcagatccg cgaggttcgc cccgttcact
     5401 tctacctgga gcgcatgttc cccgtgcgac gaccatccga gcagcccttt gaagccgccg
     5461 aatcccggat agaggagcag gaagccgacg ccccagatgt agaacgccgc ggatgcggct
     5521 acccaccagc gcggcagccg gcgcacgctt tcgcgcagca cgccgtgcgc ccagacgtgg
     5581 cccgtggtgc catcctccag caccggaatc tcgaccttct gcgcccacag gaaaagaaac
     5641 agcgtgatgc tcgcgttgaa cacgaccaga aacatcaccc acagcgacca gaacgaattc
     5701 atgggcgatc ctccgcgtcg gccatcggca gacgtgccag ccggtcgtat ttcggcttgt
     5761 gaccgtcatt ccagacccag atccagatgc cgatgaacag cagcatcatc acgacggtca
     5821 cgatgcccgc gacctggccc cacgcggcag tcacggcgcg gtctccacca ttggcgatac
     5881 gccgagacct tgcagatacg ccaccagggc atcgagcgcc gtcttgccct cgagcgctgc
     5941 gggcactccc gagatgtccg cgtcgctgta cgggtcgccg aggcgcctga gcgcacgcat
     6001 gcgcgcgctg atgtcgtcgg cgtcgagctt ctcttctgcg agccacggat aggccggcat
     6061 gttcgaagcc tgcacgacgg cgcgcggatc catgaggtgc agccgctgcc attcatccga
     6121 gtacttgccg ccgacccgcg cgagatcggg cccggtgcgc ttcgagcccc actggtgcgg
     6181 gcggtcgtag accgattcgc tcgagatcga gtaatggcca tagcgctgcg tttcggcgcg
     6241 cagcgcacgg atcatctgcg tgtggcaggt gtagcagcct tcccgcacgt agatgtcctt
     6301 gccggcgagg cgcagcggat cgtagggctt gacgccctcc accgcggtga tggcatgtgc
     6361 ctcgacgaac agcggcgtga tctcggcgag cccgccgatc gacaccatga tcgcagtcag
     6421 gatgccgagc ttgccggcgt gcttctcgac cgcttcgaag tacttgtaac ccttggccat
     6481 gtccttctcc tcaggcgcgc gccgcaacgg gcgcgggcac ctggagcggt tcgggctccg
     6541 gaatgggtac cggaatcggc ttgatcagcc gtgcgcgggc atccgccgcc gtgtgccaca
     6601 ggttccacgc catcgcgatc atgccgccga cgatcaggag accgccgagg aagcggatca
     6661 gatagaaggg ccgcacggcg atcaggctgt cgatgaaggg gtaaatgagc gcgccgttgg
     6721 catcggtcgc gcgccacatg aggccctcgg tgacacccgc cgtccacatc gcagcgacgt
     6781 acaggatgag acctgcggtg tgcatccaga agtgcagttc catcgagcgc ttcgaatgca
     6841 tcgccgggcg tccgagcgcg cgcggcgcca tcgcgtagag cgagccgatc gtgatcatcg
     6901 cgacccaacc gatcgcgccc gagtgcacgt gcgcgacggt ccaatcggtg tagtgcgaga
     6961 gcgagttgac ggttttcacc gccatcagcg agccttcggt ggtcgcagcc gcatagaaaa
     7021 cgagcgccat caccatgaat ttcgcggcgg ggtcggaggc gacccgctgc caggcgccgt
     7081 tgaacgtgaa gagaccgttc gcggccgagc cccagctcgg catcagcagc agtatcgaga
     7141 acgccatgcc caccgactgc acccagtcgg gcagtgccgt gtaaagcagg tggtgcgagc
     7201 ccgcccacat gtacaccgag atcagcgccc agaaattgac gatcgagaag cggtagctcc
     7261 acagcggctg ctgggcctgt cgcgggacga agtagtacat catcccgag
//
LOCUS       DAORUQ010000158         7307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_11530_length_7307_cov_4.702978, whole genome shotgun sequence.
ACCESSION   DAORUQ010000158 DAORUQ010000000
VERSION     DAORUQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11530_length_7307_cov_4.702978"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLW13_12480"
     CDS             complement(<1..390)
                     /locus_tag="PLW13_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQY71241.1"
                     /translation="MEGAAKRWIGAQELYEDSLRLALAVLDSGFAPTLLLGLWRGGSP
                     VAITVHETLAYFGAQAEHLPLRTSLYTGIDARAPRIRIHGIEALAVAGFDCQRVLIID
                     DVFDTGVTLEQTLDALRAVPATHASEIR"
     gene            complement(390..1250)
                     /locus_tag="PLW13_12485"
     CDS             complement(390..1250)
                     /locus_tag="PLW13_12485"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQY71242.1"
                     /translation="MDPTILSLAPFADGIGFGEAPRWHQGELWLSDIVQRQVLRVAAD
                     GQLHKVLDTPGEPSGLGWLPDGTLLVVQMQEHEVWAHAHGKQRRYSGTAPMTRVRLND
                     MVVDRNGRAWVSSFGFDYERGEAVQPTNLVCIDPGGHAWIAADNVWCPNGMAIDDSGE
                     VLFVGQSASPELLEFTIDANGVLKNRRVFGTLPPDAVADGICVDRSQALWIASPTTRE
                     FLRMERGGTITHRVPTGERIAIACVLGGPERRTLFGITAATMSLHAAHGRRDGRIGQV
                     EAPVGGAGVP"
     gene            1391..1663
                     /locus_tag="PLW13_12490"
     CDS             1391..1663
                     /locus_tag="PLW13_12490"
                     /inference="COORDINATES: protein motif:HMM:NF018334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 domain-containing protein"
                     /protein_id="HQY71243.1"
                     /translation="MKDAPAKIITLAFYAAALVSLFVTWPPLLESILQIGTLILFAAH
                     VIELVVSFRWIRLYEGPLAVSILLTLLFGFMHWMPYKRRAQQGGAR"
     gene            1671..3539
                     /locus_tag="PLW13_12495"
     CDS             1671..3539
                     /locus_tag="PLW13_12495"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY71244.1"
                     /translation="MALHRSGAGWHRQFVAALLLAAASVAAPAQDGGGCPDFNPLRNV
                     YAGDLHVHTALSLDAATQDTRARPADAYRFARGEPLAVQPYDEEGRALRELRLARALD
                     FAAVTDHAELLGEVSLCQTPGSASFGSWQCLLYRHVPRAAYYLFNYTASKTRRLGNCG
                     EDGALCREAAAGPWTEIRQAAADALDASGQCRFTSFVAYEWTGARGVDAGNMHRNVIF
                     RGDAVPRLPITFVDTGSPEKLWQLLDSECRNAVPGCEVLAIPHNSNLSDGFMFDGLRV
                     DGTPYTAADAALRARSEPLFEIMQHKGASECYAGSTRSIAADEYCGFEQLPYDAFAGQ
                     NFESRRQAPGPETGFFREILRDGLRLEQTLGTDPYRMGVIGSTDTHLGAAGGVSEADF
                     IGHGGAGVPAREGVPQGLPDRPEFGPGGLAMIWAEQNTRDALFAAMQRRETYATSGTR
                     PQLRFFGGWDFPADFCASAQLVREAYARGVPMGGELKRAAGSVAAPVFVVAALRDALE
                     GSPLQQLQLVKGWVDADGESRERVVTVASADAGADSLCAVWRDPAYEPGHSAWYYARL
                     LEMPTPRWSARICAANKVDCADPGSIGEGLEACCAPEHRASIQERAWSSPIWVSAE"
     gene            3559..4512
                     /locus_tag="PLW13_12500"
     CDS             3559..4512
                     /locus_tag="PLW13_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY71245.1"
                     /translation="MPWLNRILRYRPAHFVLAGFAGWLLLAPPAQQGEWREPVTLGPE
                     RIEALREGWAAATGREPDEAELQSLQRQEIDDEILFREALARSLHRLDPVVKQRLVLN
                     MRFLDPQTRAGDEALFEQALALGMQGNDVVVRRRLVQIMELSIQEGADRSPASETELA
                     AMFEKRREEFALPARWRITQVYFSADRRKERAPADARAALEQLRREALEPPAALALGD
                     PFLGGHQLPLLNALQLAGQFGEDFARALGACATGQWCGPIASSYGEHLVRVEEAEAGR
                     MPGLDEPDVRRRLESDVLRERARRTLAEAMVSLRQKYGVQS"
     gene            4539..5507
                     /locus_tag="PLW13_12505"
     CDS             4539..5507
                     /locus_tag="PLW13_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQY71246.1"
                     /translation="MLLLLFAGSALAHKLAPSLLAIHEQADGSYAVTWKTPRFASTPV
                     PLEPQLPASCSETGERRWEYEGTGVRIDWSMRCAEPLHGQELRVTGLAQNQSAALVRI
                     EWSDGVVAQGMLNATQPVFRIAARQDALAVAADYVKMGVGHILGGIDHLLFVLGLLLL
                     VVGWKRLVWTITAFTVGHSVTLALAALGFLRYPVELVEFAIALSIFVVAVELTREKTD
                     AHWLRHWPWLAAGGFGLLHGMGFAGALLEVGLPAHDIPLALLAFNVGIEIGQLLFVGA
                     VIAVHQAWLRLPLPRWPWLRWAPVYLIGCASAYWCFERGAAAFGGA"
     gene            5544..6026
                     /locus_tag="PLW13_12510"
     CDS             5544..6026
                     /locus_tag="PLW13_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71247.1"
                     /translation="MAKFTRAELEQALAIYNAARDAASSSGDWRIWAELFTDDAHYIE
                     HAYGEMHGRQAICDWITGVMAPFPKMTFPQDWVCFDEDNGAIVFQCQNAFPEPFDAAT
                     GKPFSFPNWTRLVYAGNGKFSSEEDIYNPARDSRNTVRGWIAAGGRFLTPEQVKMRHH
                     "
     gene            6035..6430
                     /locus_tag="PLW13_12515"
     CDS             6035..6430
                     /locus_tag="PLW13_12515"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQY71248.1"
                     /translation="MPGKLPARMPAGLRRAIRVLFAVALVLVFVLSVLKVSVGPQFRF
                     GDKVGHALVFFLLAALAQASHAERRVVARLMLALLAYGLFIELVQALLPWRDFSLWDW
                     GADGVGIALCVALAYRLRRRLGAPWRAAP"
     gene            6427..6885
                     /locus_tag="PLW13_12520"
     CDS             6427..6885
                     /locus_tag="PLW13_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQY71249.1"
                     /translation="MKKKLLVVHHSQSGSTERLAHAAVAGAREEDAVELRWLRAAEAG
                     AADLAWCDALLLATPENFGFMSGGMKEFLDRTFYAASAQQLNRACALIISAGNDGSGA
                     VRQLHRILQGYPMREVAEPLLVRGEPGPADLERAADTGRALAAGLELGIF"
     gene            6912..>7307
                     /locus_tag="PLW13_12525"
     CDS             6912..>7307
                     /locus_tag="PLW13_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQY71250.1"
                     /translation="MESAEQEVLGALAGWLREGQRAWLCTIVEISGSSPRPLGSIMAC
                     AADGRVCGSLSGGCVEEDLIERMRAGELASTGPVLRTYGLSAEENERLGLPCGGRLGV
                     VAEPCAAPTHLAHIEELLGGLAARRCVRRE"
BASE COUNT         1018 a         2429 c         2716 g         1144 t
ORIGIN      
        1 gcggatctcg gaggcgtgcg tcgcgggcac cgcgcgcagc gcatcgagcg tctgttccag
       61 cgtgacgccg gtgtcgaaca cgtcatcgat gatcagcacg cgctggcagt cgaagccggc
      121 gaccgcgagc gcctcgatgc cgtggatgcg gatgcgcggt gcgcgcgcgt cgatgccggt
      181 gtagagcgag gtgcgcagtg gcaggtgctc ggcctgcgcg ccgaagtacg cgagcgtctc
      241 gtgcaccgtg atcgccaccg gggagccgcc gcgccacagt ccgagcagca gcgtgggggc
      301 gaaaccgctg tcgagcacgg ccagagcgag gcgcagcgaa tcctcgtaga gctcctgcgc
      361 gccgatccag cgcttggcgg cgccttccat cagggcacgc ccgcgccgcc aaccggcgcc
      421 tcgacctgcc cgatgcgccc gtcgcgcctg ccgtgcgcgg cgtgcaggct catcgtcgcc
      481 gcggtgatgc cgaagagcgt gcggcgctcc ggtccgccca gcacgcaggc gatcgcgatg
      541 cgctcgcccg tcggcacgcg gtgcgtgatg gtgccgccgc gctccatgcg caggaactcg
      601 cgcgtggtgg gcgaggcgat ccacagcgcc tgcgagcggt cgacgcagat gccgtcggcg
      661 accgcgtcgg ggggcagggt gccgaacacc cggcggttct tcagcacccc gttggcgtcg
      721 atagtgaatt ccagcagttc cggcgaggcg ctctgaccga cgaacaggac ctcgccggag
      781 tcgtcgatgg ccatgccgtt ggggcaccag acattgtccg ccgcgatcca cgcgtgccca
      841 cccggatcga tgcagaccag gttggtcggc tgcacggctt cgccacgctc gtagtcgaag
      901 ccgaagctgc tgacccaggc gcgcccgttg cgatccacga ccatgtcgtt gagccgcacc
      961 cgcgtcatcg gtgcggtacc ggaatagcgg cgctgcttgc cgtgcgcgtg cgcccagacc
     1021 tcgtgctcct gcatctgcac caccagcagc gtgccgtcgg gcagccagcc caggcccgag
     1081 ggttccccgg gcgtgtcgag caccttgtgc agctgtccgt cggccgctac gcgcagcacc
     1141 tggcgctgca cgatgtcgga aagccacagt tcgccctggt gccagcgcgg ggcctcgcca
     1201 aaaccgatcc cgtcggcgaa gggggccagc gaaagtatcg tcgggtccat ggatgtcctc
     1261 ctcggcggca ttgatagcat cggcggtcga gcaggcgcaa gcgattcgtt cgcagcgcgg
     1321 cgcttccatt cgctgcgccg attgcgcacc atagcgcccg gccgaaccgg cccataacaa
     1381 ggagactgcg atgaaagacg cgcccgcgaa gatcataacc ctcgcattct atgccgcggc
     1441 actggtgtcg ctgttcgtca cctggccgcc gctgctcgag agcatcctgc agatcggcac
     1501 cctgatcctg ttcgcggcgc acgtgatcga gctggtggtg agcttcaggt ggatcaggct
     1561 ctacgagggg ccgctggcgg tcagcatcct gctgacgctg ctgttcggct tcatgcactg
     1621 gatgccctac aagcgtcgcg cccagcaggg cggggcgcga tgaaatgctg atggcattgc
     1681 accgctcggg tgcgggctgg caccggcagt tcgtggcggc actgctgctg gcggcggcat
     1741 ccgttgccgc gccggcgcag gatggcggcg gatgtcccga tttcaacccg ctgcgcaacg
     1801 tctacgccgg cgacctgcac gtgcacacgg ccctctcgct ggatgccgcg acgcaggaca
     1861 cgcgggcgcg cccggcggat gcctaccgct tcgcgcgcgg cgagccgctc gcggtgcagc
     1921 cctacgatga agagggccgg gcgctgcgcg agctgcggct cgcgcgggcg ctggatttcg
     1981 cggccgtcac cgaccatgcg gaactgctcg gtgaagtgag cctgtgccag acgcccggct
     2041 ccgccagctt cggcagctgg caatgcctgc tgtaccggca cgtgccgcgc gccgcctatt
     2101 acctgttcaa ctacaccgcg agcaagactc ggcggctcgg caactgcggc gaggacggtg
     2161 cgctctgccg cgaggccgcg gcggggccgt ggaccgagat ccgccaggcc gccgccgatg
     2221 cgctcgacgc cagcggtcag tgccgcttca ccagcttcgt ggcctacgaa tggaccggcg
     2281 cgcgtggcgt ggatgccggc aacatgcacc gcaacgtgat cttccgcggc gatgcggtgc
     2341 cgcgcttgcc gatcaccttc gtcgataccg gcagcccgga gaagctgtgg cagctgctgg
     2401 acagcgaatg ccgcaacgcg gtgccgggtt gcgaggtgct ggcgatcccg cacaactcga
     2461 acctcagcga tggcttcatg ttcgacgggc tgcgcgtcga cggcacgccc tacaccgcgg
     2521 cggacgccgc gctgcgcgca cgctcggagc cgctgttcga gatcatgcag cacaagggcg
     2581 cctccgagtg ctacgccggg agcacccgga gcatcgcggc cgacgagtac tgcggcttcg
     2641 aacaactgcc ctacgacgca ttcgcggggc agaacttcga gtccaggcgc caggcgccgg
     2701 gcccggaaac gggtttcttc cgcgagatcc tgcgcgacgg gctgcgcctc gaacaaaccc
     2761 tgggaaccga tccctaccgc atgggcgtga tcggcagcac cgacacccac ctcggcgcgg
     2821 ccggcggcgt atcggaggcg gatttcatcg gtcatggcgg tgcgggtgtg ccggcgcgcg
     2881 agggtgtgcc gcagggtctg ccggaccgtc ccgaattcgg gccgggcggg ctcgcgatga
     2941 tctgggccga gcagaacacg cgcgatgcgt tgttcgcggc gatgcagcgg cgcgagactt
     3001 acgcgaccag cggcacgcgc ccgcagctgc gcttcttcgg cggctgggat tttcccgccg
     3061 atttctgcgc cagcgcgcag ctggtgcgcg aggcctacgc gcgcggcgtg ccgatgggcg
     3121 gtgaactgaa gcgcgcggcg ggatcggtgg cggcgccggt gttcgtggtc gcggcgctgc
     3181 gcgatgcgct cgagggctcg ccgttgcagc agctgcagct cgtcaagggc tgggtcgatg
     3241 ccgatggcga gtcgcgcgag cgcgtggtga ccgtggcgtc ggcggatgcg ggcgccgatt
     3301 cgctgtgcgc ggtgtggcgc gatccggcgt acgagcccgg tcacagtgcc tggtattacg
     3361 cgcggctgct ggagatgccg acgccgcgct ggagtgcgcg catctgcgcg gcgaacaagg
     3421 tcgattgtgc cgatcccggc agcatcggcg agggtctcga ggcgtgctgc gcgcccgagc
     3481 atcgcgccag catccaggag cgggcctgga gttcgccgat ctgggtgagt gccgagtgag
     3541 cagcggggaa gcatctgcat gccgtggttg aacaggatcc tgcgctaccg gccggcgcac
     3601 ttcgtgctgg ccgggttcgc gggctggctg ctgctggcgc cgccggcgca gcagggcgag
     3661 tggcgcgagc cggtgacgct gggtcccgag cgcatcgagg cgctgcgcga gggctgggcc
     3721 gcggcgacgg gacgcgagcc ggacgaggcc gagctgcagt ccctgcagcg ccaggagatc
     3781 gacgacgaga tcctgtttcg cgaggcgctg gcgcgttcgc tgcatcgcct cgatccggtg
     3841 gtgaagcagc gcctggtgct gaacatgcgc tttctcgatc cgcaaaccag ggcaggcgat
     3901 gaagcgctct tcgagcaggc gctcgcactc ggcatgcagg gcaacgacgt ggtggtgcgc
     3961 cgccgcctgg tgcagatcat ggagctctcg atccaggagg gtgccgaccg ttcgccggcg
     4021 agcgagacgg aactcgccgc gatgttcgag aagcgtcgcg aggagttcgc gttgccggcg
     4081 cgctggcgca tcacgcaggt ctatttcagt gccgaccggc gcaaggagcg tgcgcccgcc
     4141 gatgcgcgcg ccgcgctgga gcagctgcgg cgcgaggcgc tggagccacc ggcggcgctc
     4201 gcgctcggcg atcccttcct cggcggccac cagctgccgc tgctgaacgc gctgcagctc
     4261 gcgggccagt tcggcgagga tttcgcgcgc gcgctcggcg cgtgcgcgac ggggcagtgg
     4321 tgcggcccca tcgcttcgag ctacggcgaa cacctggtgc gcgtggagga ggccgaggcc
     4381 ggccgcatgc cgggcctgga cgagcccgat gtgcggcggc gcctcgagag cgacgtgctg
     4441 cgcgagcgtg cccggcgcac gctggccgag gcgatggtgt cgctcaggca gaaatacggg
     4501 gtgcaatcat gaggcgccgg gtgcggtatt gcgcggccat gctgctgctc ctgttcgcgg
     4561 gcagcgcgct ggcccacaag ctcgcgccct cgctgctggc gatccacgag caggccgacg
     4621 ggagctacgc cgtgacgtgg aagacacccc ggtttgcctc gacgccggtg ccgctggaac
     4681 cgcaactgcc ggcctcgtgc agcgagaccg gcgaacggcg ctgggaatac gagggcaccg
     4741 gggtgcgcat cgactggagc atgcgttgcg ccgagccgtt gcacgggcag gaactgcgcg
     4801 tcaccgggct ggcgcagaac cagtccgcgg cgctggtgcg catcgagtgg agcgacggcg
     4861 tggtggcgca gggcatgctg aatgccaccc agccggtgtt ccgcatcgcg gcgcgccagg
     4921 acgcgctcgc ggtggcggcc gactacgtga agatgggcgt cgggcacatc ctcggcggca
     4981 tcgaccacct gctgttcgtg ctcgggctgc tgttgctggt cgtgggctgg aagcgcctgg
     5041 tgtggacgat caccgcgttc acggtgggcc atagtgtcac gctcgcgctc gcggcgctgg
     5101 gtttcctgcg ctacccggtg gagctggtcg agttcgcgat cgcgctcagc atcttcgtgg
     5161 tggccgtgga gctcacgcgc gagaagaccg acgcgcactg gctgcgccac tggccgtggc
     5221 tcgccgcggg cggtttcggc ttgctgcacg gcatgggttt cgcgggcgcg ctgctcgagg
     5281 tgggcctgcc ggcgcacgac atcccgctgg cgctgctcgc cttcaacgtc ggcatcgaga
     5341 tcgggcagct gctgttcgtg ggcgcggtga tcgccgtgca ccaggcgtgg ctgcgcctgc
     5401 cgctgccgcg ctggccctgg ctgcgctggg cgccggtgta cctgatcggc tgcgcctcgg
     5461 catactggtg cttcgagcgc ggcgccgccg cgttcggcgg cgcttgatca cacgcatcat
     5521 tcatcacgca ggagcacgca gacatggcga aattcacccg ggcggaactc gaacaggcgt
     5581 tggcgatcta caacgccgcg cgcgacgcgg catcgagcag cggcgactgg cgcatctggg
     5641 ccgagctgtt caccgacgat gcccattaca tcgagcacgc ctatggcgag atgcacggac
     5701 gccaggcgat ctgtgactgg atcaccgggg tgatggcgcc gttcccgaag atgaccttcc
     5761 cgcaggactg ggtgtgcttc gacgaggaca acggggcgat cgtgttccag tgccagaacg
     5821 cctttcccga acctttcgat gccgccaccg gcaagccctt ttcgttcccg aactggacgc
     5881 ggctggtcta cgccggcaac ggcaagttct ccagcgagga ggatatctac aacccggcgc
     5941 gcgattcgcg caacacggtg cgcggctgga tcgcggcggg cggcaggttc ctgacacccg
     6001 agcaggtcaa gatgcgccac cattgagcgg ccccatgccc gggaagttgc ccgcgcggat
     6061 gcctgccgga ctgcgccgtg ccatccgtgt gctgtttgcg gtggcgctgg tgctggtgtt
     6121 cgtgctctcg gtgctgaagg tgtcggtggg gccgcagttt cgcttcggtg acaaggtcgg
     6181 acacgcgctc gtgttcttcc tgctcgccgc gctcgcgcag gccagccatg ccgagcggcg
     6241 cgtcgtcgcg cgcctgatgc tggcgctgct ggcttacggg ctgttcatcg agctggtgca
     6301 ggcgctgctg ccctggcgtg acttctcgct gtgggactgg ggcgccgatg gcgtcggcat
     6361 cgcgctgtgc gtggcgctcg cgtaccggct gcgccggcgc ctcggcgcac cgtggcgggc
     6421 ggcgccgtga agaagaagct gctcgtcgtg caccactccc agagcggcag caccgaacgc
     6481 ctggcgcatg cggcggtggc gggcgcgcgc gaggaagacg ccgtggagct gcgctggctg
     6541 cgcgccgccg aggcgggcgc ggcggacctc gcgtggtgcg acgcgctgct gctggcgacg
     6601 ccggagaatt tcggtttcat gtccggcggc atgaaggaat tcctcgaccg cacgttctac
     6661 gcggcctcgg cgcagcaact gaaccgggct tgcgcactga tcatcagcgc gggcaacgac
     6721 ggcagcgggg cggtgcgcca gttgcaccgc atcctccagg gttatccgat gcgcgaggtg
     6781 gcggagccgc tgctcgtgcg cggcgagccc ggacccgcgg atctcgagcg tgccgccgac
     6841 accgggcgcg cgctggcagc gggcctggag ctcggtattt tctgaaggac attcgagggc
     6901 gggcgatgca ggtggaatcg gcggaacagg aagtgttggg ggcgctggcc ggctggctgc
     6961 gcgagggaca gcgcgcgtgg ttgtgcacca tcgtcgagat cagcggctcc tcgccgcgtc
     7021 cgctcggttc gatcatggcc tgcgccgccg acgggcgcgt ctgcggctcg ctctcgggcg
     7081 gctgcgtcga ggaggacctg atcgagcgga tgcgcgccgg cgagctcgcg agcacgggcc
     7141 cggtgctgcg tacctacggc ctgagcgctg aggagaacga gcgcctcggc ctgccctgcg
     7201 gcggacggct cggcgtggtg gcggagcctt gcgcggcgcc gacgcatctg gcccacatcg
     7261 aggaactgct gggcggactc gcggcgcggc gctgcgtgcg ccgcgag
//
LOCUS       DAORUQ010000159         7112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12027_length_7112_cov_4.097067, whole genome shotgun sequence.
ACCESSION   DAORUQ010000159 DAORUQ010000000
VERSION     DAORUQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12027_length_7112_cov_4.097067"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLW13_12530"
     CDS             complement(<1..370)
                     /locus_tag="PLW13_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY71251.1"
                     /translation="MHFAAADYDTLVFDCDGVILDSNRIKSAAFRTVTAPFGAEASAR
                     FLDYHHRHGGISRNEKFAHFLDAIVTVAPADRERLLGGLLSDYAAICARELLACPLIP
                     GVAALLASIPPRVAAHVVTGG"
     gene            complement(351..1094)
                     /locus_tag="PLW13_12535"
     CDS             complement(351..1094)
                     /locus_tag="PLW13_12535"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQY71252.1"
                     /translation="MPATARSDCAIVIPARFASSRFPGKPLADILGKPMIRHVWERCV
                     QALGPEHVYVATDDARIRAACEGFGARVLMTSSACLTGTDRVAEAARALDYEFLINVQ
                     GDEPMLDPADILAVAQAWRCHGGAVINAMTAIAEEREYWSRNVPKVVATQDGRLMYMS
                     RAAIPANKGGSFEKAWKQVCIYCLSPAQLAAFASVSAKGPIEQIEDIEILRFLDLGVE
                     VRMVELASTSIAVDTPEDLERVVDALRGG"
     gene            complement(1078..2703)
                     /locus_tag="PLW13_12540"
     CDS             complement(1078..2703)
                     /locus_tag="PLW13_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HQY71253.1"
                     /translation="MTLRILDCTLRDGGYYNNWCFARELAEEYLQAMAAAGVDIVEIG
                     FRNFPQASFLGPYAYSTDAFLDTLALPAGPALAVMVDAKALLGCGLAPAAAVDALFQP
                     RDRSRVEWVRIAAHLGEIASCRALAARLRELGYRVGLNMMQAAGKPAGQITTLAREVA
                     SWGAVEVLYFADSFGSMDGTEVGRVVDALAAGWNGAIGIHTHNNKSLAVRNTLAAIER
                     GVSFLDATVTGMGRGAGNAELEILLCELDARGIKASRLEELSSLCVNGFQPLKDQYGW
                     GASFFYHYSALHDIHPMFAQTLMADPRYSAREKFSALRSLAARNSTSFSLTEVERSLH
                     SFRDDRPLDASAPPTDLSAFRDADVLLVGAGPSVQQYAADIELFIRDHRLLVLTLNHQ
                     GAIDPALVDGIICVDQFRLLYEADFLAGCGKPIYSARRFQDEGTRAALGAAELHEYDC
                     VLQPGGFEARANGCVIPVPLAFGYALALCIAGRARRIFLVGFDGFHADDSRQAEMLQL
                     FRVVAPAISDIALTALTPTNYPVAQGSIYASYR"
     gene            complement(2843..3967)
                     /locus_tag="PLW13_12545"
     CDS             complement(2843..3967)
                     /locus_tag="PLW13_12545"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HQY71254.1"
                     /translation="MHDTEPKQATGAQPLIWLLLADKQGDNKQVFSLADNLGCPYETR
                     FVYPKPRFAKGKPFFRPSVAHIDLARSDAIAPPWPDVIITIGRRPTMAALWIRRRSGG
                     HTRIILIGRQPRYLREFALVIASCQYITANDPRIVRIALPLDDGLRRVNAPEGAVAAP
                     AGSDAATETALLIGGPTRSFRLGVPEAREMLRLATLHGGGAPLRIVTSRRTPADVCAW
                     FSANLPGGSRLSAWQPGAHTTSDYAQALAQCARFIVTGDSVSMICDVVRERKALAIYR
                     LPMRNALYERWHRLILAAAPIDDGRVRTGWLAALARRIVDSRLIRLPRSFDAFYSYLF
                     RHGSASDLAEGFLPAYSFTPEQDLERATQHARRVVQACRG"
     gene            complement(3967..4893)
                     /locus_tag="PLW13_12550"
     CDS             complement(3967..4893)
                     /locus_tag="PLW13_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71255.1"
                     /translation="MPVFGPLYQGDERARAALAALLDALQTRHAGAVDAVLFYGSCLR
                     SGDLHDGLADLYVIVRDYRGAHRTLRAAAANRALAPNVYYLQTGTANGMVRCKYAVLS
                     MADLERGVSRNWFESYIWGRFCQPVAVAHHRDETTLERVRAALRTATITFLDRALPAI
                     AASGTVASLWTDALALSYATELRAESGKRSGTLVTMGAEYFSAATREVATALRWPLAL
                     DGERYAATIPPAQRRAAAVSWGLRRVQGKLLSVARLLKALLTFEGGFDYIAWKLERHS
                     GRPVVIPPRVRKYPLIFVWGLMWRLYREGILR"
     gene            complement(4897..5910)
                     /locus_tag="PLW13_12555"
     CDS             complement(4897..5910)
                     /locus_tag="PLW13_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQY71256.1"
                     /translation="MHATTPATARGLPARPGAAPRLGVITNPHSGRNRRQLAEVRRLL
                     ETSGRVRHIVTEDSAAIPHALAAFADEGVGIVGINGGDGSVAHVLTTLMHASPFASPP
                     LLCALPGGTTNVTVGDVGIRGGLCAAILALLGWLHSGDARALVVQRPVIGVSGAGGAP
                     LGCGLVFGAGAVVDGIEYWQEQVRARGMRSELSSGVAMIRTLWGMVHGHDGFGEALRI
                     GITPAGSARSDGEFMLLAISALERLFLGIHPFWGGGDGRLYFTAIERHAAHFARAVPS
                     LLRGRAAAFMSRDNGYHSARLDSVQLDFRSAFTLDGELHHPAPEDAPMRVGCAGTARF
                     LRI"
     gene            complement(5952..6866)
                     /locus_tag="PLW13_12560"
     CDS             complement(5952..6866)
                     /locus_tag="PLW13_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71257.1"
                     /translation="MTSTHVQLSEQRPSASARLRIAGLLLALFAAPALAGGTQCWREL
                     RFSASNGWLGASSTLRYEPPVPVARAGLSAPKQGEALQPRGAQVGRIHATFAAMSNTG
                     ELDTWFDPQTLGVLQTRRLSQGKNSRLKTHRFLGNGVWRERLEPGDEARWTARSAALI
                     PYPAHPDAGAPVLTPVMLVARAAELVRAGRKTSDQLVFSDTQLYHVRLAASATDSIDV
                     NLRLVVDGKERRVREPRQAQRVAVMPRLLGTAAEKEAFSLLELTGDLAIFIDTESGLP
                     LRIQGTWLRVGTVPADLARAELVPGCAG"
BASE COUNT         1100 a         2397 c         2578 g         1037 t
ORIGIN      
        1 cgccgcccgt gaccacgtgc gccgcgacgc gcggcgggat cgaggcgagc agcgccgcga
       61 cgccgggtat caaagggcag gcgagcagtt cgcgcgcgca gatggcggca tagtcgctca
      121 gcaggccgcc gagcaggcgt tcgcggtctg cgggagccac cgtgacgatc gcgtcgagga
      181 aatgggcgaa cttctcgttg cggctgatgc cgccgtggcg gtggtggtag tcgagaaagc
      241 gcgcgctcgc ctcggcgcca aaaggtgcgg tgacggtgcg aaaggccgcg ctcttgatgc
      301 ggttggaatc gaggatgacc ccgtcgcagt cgaagaccag tgtgtcgtaa tcagccgccg
      361 cgaagtgcat ccaccacgcg ctccaggtcc tcgggcgtgt cgaccgcaat gctggtcgag
      421 gccagttcga ccatgcgcac ctcgacgccg aggtcgagga agcgcaggat ctcgatgtcc
      481 tcgatctgct cgatcggtcc cttcgcgctc acgctggcga aggccgcgag ctgcgccggc
      541 gagaggcagt agatgcatac ctgcttccac gccttctcga agctgccgcc cttgttcgcc
      601 gggattgccg cgcggctcat gtacatcagg cgcccgtcct gcgtggcgac cactttgggg
      661 acgttgcgcg accagtactc gcgctcctcg gcgatggcgg tcatcgcgtt gatcaccgcg
      721 ccaccgtggc accgccaggc ctgcgccacg gcgaggatgt cggccgggtc cagcatcggc
      781 tcgtcgccct gcacgttgat caggaactcg tagtcgagcg cgcgcgcggc ctcggccacg
      841 cggtcggtgc ccgtcaggca cgcggaagag gtcatcagca cgcgcgcgcc gaagccctca
      901 caggccgcgc ggatgcgcgc atcgtcggtg gcgacgtaga cgtgctcggg ccccagcgcc
      961 tggacgcagc gctcccagac gtggcggatc atcggcttgc cgaggatgtc ggcgagcggc
     1021 ttgccgggaa agcgcgagga ggcgaagcgc gccggaatga cgatcgcgca atcggaccta
     1081 gcggtagctg gcatagatcg atccttgggc cacggggtag ttggtgggcg tcagcgcggt
     1141 cagcgcgatg tcgctgatcg cgggcgccac gaccctgaac agctgcagca tctcggcctg
     1201 gcgcgagtca tcggcatgga atccgtcgaa gccgaccagg aagatacgcc tggcgcgccc
     1261 ggcgatgcac agcgccagcg cgtagccgaa ggcgagcggc accgggatca cgcaaccgtt
     1321 ggcgcgcgcc tcgaagccgc cgggttgcag cacacaatcg tactcgtgca attcggccgc
     1381 gccgagcgcc gcgcgcgtgc cctcgtcctg gaagcggcgc gctgaataga tcggcttgcc
     1441 gcagccggcg aggaaatcgg cctcgtacag cagccggaac tggtcgacgc agatgatgcc
     1501 atcgaccagc gcgggatcga tcgcgccctg gtggttcagc gtcagcacca gcagccggtg
     1561 gtcgcggatg aagagctcga tgtcggcggc atactgctgc acgctgggtc ccgcgccgac
     1621 cagcagcacg tcggcatcgc ggaacgcgga gaggtccgtg ggcggcgccg aggcgtcgag
     1681 cggacgatca tcgcgaaagg aatgcagcga gcgctcgacc tcggtcaggc tgaagctggt
     1741 cgagttgcgc gcggccagcg agcgcagcgc ggagaacttc tcgcgcgcgc tgtagcgcgg
     1801 gtcggccatc agtgtctgcg cgaacatcgg gtggatgtcg tgcagcgccg agtaatggta
     1861 gaagaagctc gcgccccagc cgtactggtc cttgagcggc tggaagccgt tcacgcacag
     1921 cgagctcagc tcctcgaggc gcgaggcctt gattccgcgc gcgtccagtt cgcacagcag
     1981 gatttcgagc tcggcgttgc cggcgccgcg ccccatgccg gtcacggtcg cgtcgaggaa
     2041 gctcacgccg cgctcgatcg cggccagcgt gtttcgcact gccagcgact tgttgttgtg
     2101 ggtgtggatg ccgatggcgc cattccagcc ggcggccagc gcatcgacca cgcgcccgac
     2161 ctcggtgcca tccatcgagc cgaaggaatc ggcgaagtag agcacctcga ccgcgcccca
     2221 cgacgccact tcgcgcgcga gcgtggtgat ctgcccggcg ggcttgccgg cggcctgcat
     2281 catgttcagc ccgacgcggt agcccagctc gcgcaggcgt gccgccagcg cgcgacagct
     2341 cgcgatttcg cccaggtgcg ctgcgatgcg cacccattcg acgcgggagc gatcgcgcgg
     2401 ctgaaacagc gcatcgacgg cggcagccgg cgcaagaccg cagccgagca gcgccttggc
     2461 gtcgaccatc accgcgagcg cgggaccggc gggcaaggcc agggtgtcca ggaacgcgtc
     2521 ggtcgaatag gcgtaggggc cgaggaagct tgcctgcggg aaattgcgga aaccgatctc
     2581 gacgatgtcg acgcccgccg ccgccatggc ctgcaggtac tcctcggcga gctcgcgcgc
     2641 aaagcaccag ttgttgtagt agccgccgtc gcggagcgtg cagtcgagaa tgcggagcgt
     2701 catggcgaat gcgtaccgga tgggccgaaa cggcgcggcc tgcagcggcg cgcgtcatgg
     2761 gtgactcgac gcattatatg ggccgtccgt ggcaccggac acccctcggc ggcggtagaa
     2821 attgcaggcc gccgcgcgcg cctcagccgc ggcaggcttg cacgacgcgt cgcgcatgct
     2881 gcgtggcgcg ctccaggtcc tgctccggcg tgaagctgta ggcgggcagg aacccctcgg
     2941 cgagatccga agccgagccg tggcgaaaga gataggaata gaacgcgtcg aagctgcgcg
     3001 gcaggcgtat cagccggctg tcgacgatgc gcctggccag tgcggccagc cagcccgtgc
     3061 gcacgcgccc gtcgtcgatc ggggcggcgg cgaggatcag ccggtgccag cgctcgtaga
     3121 gcgcgttgcg catcggcaag cggtatatcg cgagcgcctt tctctcgcgc accacgtcgc
     3181 agatcatcga gacgctgtcg ccggtgacga tgaagcgtgc gcactgcgcc agcgcctggg
     3241 cgtagtcgga ggtcgtgtgc gcgccgggct gccaggcaga cagccgggag ccgcccggca
     3301 ggttggcgct gaaccacgcg cagacatcgg cgggcgtgcg ccggctggtc acgatgcgaa
     3361 gcggcgcacc gccgccgtgc agcgtggcca ggcgcagcat ttcccgtgcc tcgggtacgc
     3421 cgaggcggaa gctgcgcgtg gggccgccga tgagcagggc cgtttccgtg gcagcgtcgg
     3481 agccggctgg cgcggcaacc gcgccctcgg gcgcgttgac gcgccgcaat ccgtcgtcca
     3541 gcggcagcgc gatgcgcacg atgcgcggat cgttcgcggt gatgtactgg cacgaggcga
     3601 tcaccagcgc gaattcgcgc aggtagcgcg gttggcgccc gatcaggatg atgcgcgtgt
     3661 gcccccccga gcggcgccgt atccacagcg cggccatcgt cgggcggcgg ccgatcgtga
     3721 tgatcacgtc cggccacggc ggcgcgatcg cgtcggagcg cgcgaggtcg atgtgcgcga
     3781 cgctggggcg aaagaacggc ttgcccttcg cgaacctggg cttcggatag acgaagcgcg
     3841 tctcgtacgg gcagccgaga ttgtcggcca ggctgaagac ctgcttgttg tcgccctgct
     3901 tgtcggctag cagcagccag atcagtggct gcgcgccggt cgcctgcttt ggttcggtgt
     3961 cgtgcatcag cgcaggattc cctcgcggta gaggcgccac atcaggcccc agacgaagat
     4021 cagcgggtac ttgcgcaccc gcggcgggat caccacgggc ctgccgctgt ggcgttcgag
     4081 cttccacgcg atgtagtcga agccgccctc gaaggtgagc agcgccttca gcagccgcgc
     4141 gacggacagc agcttgccct gcacgcgccg cagtccccag gagactgcgg ccgcgcgtcg
     4201 ctgcgccggc ggtatcgttg ccgcatagcg ctcgccatcg agcgcgagcg gccaacgcag
     4261 cgccgtcgcg acctcgcgcg tagcggcgct gaaatactcc gcgcccatcg tcaccagcgt
     4321 tcccgagcgc ttgccgctct cggcgcgaag ctcggtcgcg taactcagcg cgagcgcatc
     4381 ggtccacagt gaagcgaccg tgccactcgc ggctatcgct ggcagcgcgc gatcgaggaa
     4441 ggtgatcgtg gccgtgcgca gcgccgcgcg cacccgttcc agcgtggtct cgtcgcgatg
     4501 gtgcgcaacc gccacgggct ggcagaagcg gccccagatg taggactcga accagttgcg
     4561 cgagacgccg cgctcgaggt ccgccatgga gagcacggca tacttgcagc gcaccatacc
     4621 gtttgcagtc ccggtctgca ggtagtagac gttcggcgcc agcgcgcggt tcgcggccgc
     4681 cgcgcgcagc gtgcggtgcg cgccgcgata gtcgcgcacg atcacgtaca ggtctgccag
     4741 cccgtcgtgc aggtcgccgc tgcgcaggca ggaaccgtag aacagcaccg catcgacggc
     4801 gccggcatga cgcgtttgca gcgcgtcgag caacgccgcg agcgcggcgc gtgcgcgctc
     4861 gtcgccctgg tacagcggac cgaacaccgg catggatcat atcctcagga agcgggccgt
     4921 gcccgcgcag ccgacacgca tcggcgcgtc ttccggcgcc gggtggtgca gttcgccgtc
     4981 tagcgtgaag gcgctgcgga agtcgagttg caccgaatcc agccgcgcgc tgtggtagcc
     5041 gttgtcgcgg ctcatgaacg ccgcggcgcg gccgcgcaac agcgagggca cggcgcgcgc
     5101 gaagtgcgcg gcgtggcgtt ctatcgcggt gaaatacagg cgcccgtcgc cgccgcccca
     5161 gaaagggtgt atgccgagaa acaggcgctc cagcgcgctt atcgccagca gcatgaattc
     5221 gccgtcgctg cgcgccgagc cggcgggcgt gatgccgatg cgcagggcct cgccgaagcc
     5281 gtcgtgacca tggaccatgc cccacagcgt gcggatcatc gccacgccgg agctcagttc
     5341 gctgcgcatg ccgcgcgcgc gcacctgctc ctgccagtac tcgatgccgt cgaccaccgc
     5401 gcccgcgccg aataccagcc cgcagccgag tggcgcgccg ccggcgccgc tcacgccgat
     5461 cacggggcgc tgcacgacca gcgcccgggc atcgccgctg tgcagccacc ccagcagcgc
     5521 caggatcgcc gcgcacaggc cgccgcggat gcccacatca cccaccgtca cgttcgtggt
     5581 gccgccgggc agtgcgcaca gcagcggtgg cgaggcgaag ggcgaggcgt gcatcagcgt
     5641 cgtcagtacg tgcgccacgc tgccgtcgcc gccgttgatg ccgacgatgc cgacaccttc
     5701 gtcggcgaac gcggccagcg cgtgtggtat tgcggcactg tcctcggtga cgatgtggcg
     5761 cacgcggccg gaggtttcca gcagccggcg tacctcggcc agctgacggc ggttgcgccc
     5821 gctgtgcggg ttggtgatga cgcccaggcg cggcgcggcg cccggtcggg ccggtaatcc
     5881 gcgggcggtg gcgggcgtgg ttgcgtgcat gatggctgcg agtatagccg cgcggcgctc
     5941 ggccaacggg gctagccggc gcagccgggc acgagttcgg cgcgagccag gtccgcgggg
     6001 acggtgccca cccgcagcca ggtgccctgg atgcgcagcg gcagtccgct ctcggtatcg
     6061 atgaagatcg cgaggtcccc ggtcagttcc agcaggctga aggcttcctt ctccgcggcg
     6121 gtcccgagca ggcgcggcat cacggcgaca cgctgcgcct gccgcggttc acggacgcgg
     6181 cgttccttcc cgtcgacgac gaggcgcagg ttgacgtcga tgctgtcggt ggccgacgcg
     6241 gcgagccgca cgtggtagag ctgcgtgtcg ctgaacacca gctgatcgga ggtcttgcgg
     6301 ccggcgcgaa ccagttcggc cgcgcgcgcc accagcatca ccggcgtgag cacgggagcg
     6361 ccggcgtcgg ggtgagcggg ataggggatc agcgcggcgc tgcgcgcggt ccaccgggcc
     6421 tcgtccccgg gctccagccg ctcgcgccag acgccgttgc cgaggaagcg gtgggtcttg
     6481 aggcgactgt tcttgccctg gctcaggcgc cgggtttgca gaacccccag cgtctgcggg
     6541 tcgaaccagg tgtccagctc gccggtgttg ctcatcgccg cgaaggtcgc gtggatcctg
     6601 ccgacctgcg ctccccgcgg ttgcagcgcc tcgccttgct tcggcgcgga gaggcccgcg
     6661 cgcgccactg gcacgggcgg ttcgtatcgc agcgtcgacg aggcgccgag ccagccgttg
     6721 ctggccgaga agcgcagctc gcgccagcac tgtgtgccgc cggcgagggc aggcgcggcg
     6781 aacagcgcga gcagcagacc ggcgatgcga aggcgcgccg aggcggacgg tcgttgttcc
     6841 gatagctgga cgtgcgttga ggtcatggcg gctattgaag ccgatcagcg ggggattgca
     6901 cggtaatttt ccgggttgcc gtgaacgcgc cggatgccgg tgccagccgt ggaccgggct
     6961 cgaggctgta ttcgccggtt ccgcaaccgt ggcctcgtgg cgcaggagcc agaacgaaaa
     7021 aggggccagc ttgcgctgcc cccttcgtct accactgcaa tgtggctccg cctgttgggc
     7081 tcgaaccaac gacccgctga ttaacagtca gc
//
LOCUS       DAORUQ010000160         7019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12282_length_7019_cov_6.494687, whole genome shotgun sequence.
ACCESSION   DAORUQ010000160 DAORUQ010000000
VERSION     DAORUQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12282_length_7019_cov_6.494687"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..3113
                     /gene="uvrA"
                     /locus_tag="PLW13_12565"
     CDS             270..3113
                     /gene="uvrA"
                     /locus_tag="PLW13_12565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY71258.1"
                     /translation="MATIQVRGARTHNLKNVDLDIPRDQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSTYARQFLSVMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARAGEPRCPQHGLALTAQTVSQMVDAVLAMPEGAKLMLLAPVVRERKG
                     EHAQLLEQLRGSGFVRVRVDGRVAELDDVPALDKQRKHTIEVVVDRFRVRADLGLRLA
                     ESFETALGLAEGLAIIAPMDEPGEEQVFSARFACPHCGHSIPALEPRLFSFNNPAGAC
                     PSCDGLGVQQFFDVERIVQNPGASLAQGAIRGWDRRNVYYFHLLGSLAEHYGFSPDTP
                     FAELAPEQRERVLYGSGEEAIRFNYVNDRGDVIQRTHHFEGIVNNLERRYRETDSALV
                     REELAKFIGTRRCGACQGSRLRPEARHVFVDDRSLPQLSALGVGEAQAYFENLELAGR
                     RGQIADKILKELRSRLGFLVNVGLEYLTLDRSADTLSGGEAQRIRLASQIGAGLVGVM
                     YILDEPSIGLHQRDNERLLATLTHLRDIGNTVIVVEHDEDAIRAADYLIDIGPGAGAH
                     GGEIVAAGSAVDIMAAPRSVTGDYLAGRRAIALPARRTPRDPKRQLVLRGARGNNLKN
                     VTASIPVGLFTCVTGVSGSGKSTLVNNTLYPLAATALNGASTLDAATHGAIAGLEHFD
                     KCIDIDQSPIGRTPRSNPATYTGLFTPIRELFAGTPEARSRGYGPGRFSFNVKGGRCE
                     ACQGDGVTRVEMHFLPDVYVPCDICKGQRYNRETLDILYKGRNIRDVLEMTIEEALAF
                     FSAVPALARKLETLMEVGLSYIHLGQAATTLSGGEAQRVKLSRELSKRDTGKTLYILD
                     EPTTGLHFHDVQQLLNVLHRLRDHGNTVVVIEHNLDVIKTADWILDLGPEGGDGGGRI
                     VAEGTPEQVAANAASHTGTFLAQLLSSAKRAVP"
     gene            3282..4289
                     /gene="galE"
                     /locus_tag="PLW13_12570"
     CDS             3282..4289
                     /gene="galE"
                     /locus_tag="PLW13_12570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQY71259.1"
                     /translation="MKILVTGGAGYIGSHTCVELLAAGHEVVVVDNLANSSAEALERV
                     RLISGRPLGFHRLDLREGAALREVFARSHFDAVIHFAGLKAVGESVQKPLHYYDNNVT
                     GTLRLLECMREAGLKRLVFSSSATVYGDPASVPIREDFALSASNPYGRTKLFIEEILR
                     DEQRANPDWDIVLLRYFNPVGAHASGLIGEDPAGIPNNLMPYVSQVAVGQLDCLNVWG
                     NDYPTPDGTGVRDYIHVVDLALGHLAALERAAGPGVRAYNLGTGRGYSVLEMIAAFER
                     ASGNKVPYRFAPRRPGDVAACYADPALARQQLGWTAERDVDAMCRDTWHWQSRNPRGY
                     G"
     gene            complement(4431..5333)
                     /locus_tag="PLW13_12575"
     CDS             complement(4431..5333)
                     /locus_tag="PLW13_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HQY71260.1"
                     /translation="MRWTIAIIAPLAALSAAAGAPVWADTSWAFTCTTGCGTSSGTAG
                     NTRDATSAGITVTASAFSNTGGTSNTLLETAYLSWWNDGDGNAPPTGGLGVTNRDRCT
                     TSGCSGDYNEGSSPEHGVDNNGRWDAVLFEFSEGINLTDIGLSWYSNDADISVLAYQP
                     DAPGGAAYVKPATDPTLVGRTYGGTSTGLLANGWSLVGNYADLQTRPNWTADLSTNLV
                     SSYWLVMAYNTVFSGCNDNSQMSGDGCTNGTTSHYDYMKIGLLAGTQPGVPPSGNAPE
                     PGTMLLFGAGLAGLSLVRRRKVAA"
     gene            complement(5475..6926)
                     /gene="prsT"
                     /locus_tag="PLW13_12580"
     CDS             complement(5475..6926)
                     /gene="prsT"
                     /locus_tag="PLW13_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HQY71261.1"
                     /translation="MTVMYIKLGEFEAAVSMAGKVAERDQGAPGWNLLGVARQAGGDK
                     PGARAAYLQALALDAAFLPAELNLTRLDVEEGNLAAARDRLQRILQKRPEDPQATFEL
                     AALEEAAGNDTEALRWLEKLHANDHRNVPATARLVDLLLRGGMKEKALTVARETSAVA
                     PEDLAALAALGKVHVALGDDKQAQVFFDRMTRLAAFDAGWQHRIAQYQLSANNPEGAI
                     HSLERALESEPRHLPALVLLTEVELQLSRFEQAEQRARSLAADQATESTGQRLLGDIA
                     LAQGRAEEAVASYQSALRGQPDPELALRLYRAYFAVGQQDDAVEFMESWSRDHPQDAL
                     ARRALAEGYLRAGRLPQARSSYEALLRDQPDAPDLLNNLANVLARQGDPRALEYAQRA
                     HDLAPQDAAIRDTLGWLLVGSGQAEAGLRHLREARLRAPQNPTIRYHLAAALAAIGRE
                     DEARTELGEALSTAAEFDELEDARKLQQRLGSR"
BASE COUNT         1160 a         2395 c         2331 g         1133 t
ORIGIN      
        1 gtaggccttg aagcaggtga gttcgggggt ggtcatgcgg aagggtcctt ggtggtagcg
       61 atacgtgccg cggcaacggt cgccgggaac tataggaagc gaacgcacag ttgtcatggt
      121 gggcaggcgg cggccgcgcg cggcacgggt tcgcccctcg gcgcgccggg tacaccgccg
      181 tcgcgcaggg cgcgctccca caatgggcca tgcctgcaac agggcgacgc gggtatactc
      241 gcaggtttgg tacccctcac cggaaagcga tggccaccat acaggtacgc ggtgcgcgca
      301 cccacaacct gaagaacgtc gatctcgaca tcccgcgcga ccagctcatc gtgattaccg
      361 ggctgtcggg ctcgggcaag tcctcgctcg ccttcgacac gctgtatgcc gagggccagc
      421 ggcgctatgt cgaatcgctg tcgacctacg cgcggcagtt cctgtcggtg atggagaagc
      481 ccgacgtcga ccacatcgag ggcctgtcgc cggcgatctc gatcgagcag aaatcgacct
      541 cgcacaaccc gcgctccacc gtgggcacca tcaccgagat ccacgactac ctgcgcctgc
      601 tgttcgcgcg tgccggcgag ccgcgctgcc cgcagcacgg gctggcgctg accgcgcaga
      661 ccgtgagcca gatggtcgac gcggtgctgg cgatgccgga gggcgcgaag ctgatgctgc
      721 tggcgcccgt ggtgcgcgag cgcaagggcg agcacgcgca gttgctggag cagctgcgcg
      781 gcagcggctt cgtgcgcgtg cgcgtcgacg ggcgcgtggc cgagctcgac gacgtgcccg
      841 cgctcgacaa gcagcgcaag cacaccatcg aggtggtggt cgatcgcttt cgcgtgcgcg
      901 ccgacctcgg gctgcgcctc gcggaatcct tcgagaccgc gctggggctg gccgaggggc
      961 tcgcgatcat cgccccgatg gacgagccgg gcgaggagca ggtgttctcg gcgcgctttg
     1021 cctgcccgca ctgcggccac agcatcccgg cgctggagcc gcggctgttc tcgttcaaca
     1081 acccggccgg ggcctgcccg agctgcgacg ggctcggggt gcagcagttc ttcgacgtgg
     1141 agcgcatcgt gcagaacccc ggcgccagcc tcgcgcaggg cgcgatccgc ggctgggacc
     1201 ggcgcaacgt ctattacttc cacctgctcg gctcgctggc cgagcactac ggtttcagcc
     1261 cggacacgcc cttcgccgag ctggcgcccg agcaacgcga gcgcgtgctc tacggcagcg
     1321 gcgaggaggc catccgtttc aactacgtga acgaccgcgg cgacgtgatc cagcgcacgc
     1381 accacttcga aggcatcgtg aacaacctcg agcgccgcta ccgcgagacc gattcggcgc
     1441 tggtgcgcga ggagctggcg aagttcatcg gcacgcgccg ctgcggggcc tgccagggca
     1501 gccgcctgcg ccccgaggcg cgccacgtct tcgtcgacga ccgttcgctg ccgcagctgt
     1561 ccgcgctcgg ggtgggcgag gcgcaggcct atttcgaaaa tctggagctc gccggccggc
     1621 gtggccagat cgccgacaag atcctgaagg aactgcgcag ccgcctcggc ttcctggtca
     1681 acgtggggct cgagtacctc acgctggacc gcagcgccga cacgctctcc ggcggcgagg
     1741 cgcagcgcat ccgcctggcg agccagatcg gcgcggggct ggtcggcgtg atgtacatcc
     1801 tcgacgagcc ctcgatcggg ctgcaccagc gcgacaacga gcggctgctg gccacgctga
     1861 cgcacctgcg cgacatcggc aacacggtga tcgtggtcga gcacgacgag gacgcgatcc
     1921 gggcagccga ttacctcatc gacatcggtc ccggcgcggg cgcgcacggc ggcgagatcg
     1981 tggccgcggg cagcgcggtg gacatcatgg ccgcgccgcg ctccgtcacc ggcgactacc
     2041 tcgccgggcg ccgcgcgatc gcgctgccgg cgcgacgcac gccacgcgac cccaagcgcc
     2101 agctggtgct gcgcggcgcc aggggcaaca acctcaagaa cgtgaccgcg tcgatcccgg
     2161 tcggtctgtt cacctgcgtg accggggtgt cgggctccgg caagtccacc ctggtcaaca
     2221 acacgctgta cccgctggcc gccaccgcgc tgaacggcgc cagcacgctg gacgccgcga
     2281 cgcacggcgc gatcgcggga ctcgagcact tcgacaagtg catcgacatc gaccagagcc
     2341 cgatcgggcg cacgccgcgc tccaacccgg cgacctacac cggccttttc accccgatcc
     2401 gcgaactgtt cgccggcacc ccggaggcac gctcgcgcgg ctacgggccg gggcgcttca
     2461 gcttcaacgt gaagggcggg cgctgcgagg cctgccaggg cgacggcgtg acgcgggtgg
     2521 aaatgcactt cctgcccgac gtctacgtgc cctgcgacat ctgcaagggg cagcgctaca
     2581 accgcgagac gctcgacatc ctgtacaagg gccgcaacat ccgcgacgtg ctggaaatga
     2641 ccatcgagga ggcgctcgcc ttcttcagcg cggtacccgc gctcgcgcgc aagctcgaga
     2701 cgctgatgga ggtggggctg tcctacatcc acctcgggca ggccgcgacc acgctctcgg
     2761 gcggcgaggc gcagcgcgtg aagctctcgc gcgagctctc caagcgcgac accggcaaga
     2821 cgttgtacat tctcgacgag ccgaccacgg ggctgcactt ccacgacgtg cagcagctgc
     2881 tgaacgtgct gcaccgcctg cgcgaccacg gcaacacggt ggtcgtgatc gagcacaatc
     2941 tcgacgtgat caagaccgcg gactggatcc tcgacctagg gcccgagggc ggcgacggcg
     3001 gcgggcgcat cgtggcggag ggcacgccgg agcaggtggc cgcgaacgcg gcctcgcaca
     3061 ccggaacatt cctcgcgcag ctgctcagct cggccaagcg cgccgtgcca tgaatatgca
     3121 cgtaggtccg gattcatccg gacaattgct tcgtaggtcc ggattcatcc ggacaattgc
     3181 ttcgtaggtc cggattcatc cggacgattg tccgcctgaa ggcggaccta cggcgctgca
     3241 caatgtccgg ataaatccgg acccacgggg gacggcgagg gatgaagatt ctggtcaccg
     3301 gcggcgccgg ctatatcggc tcgcacacct gtgtcgagct gctggcggcg ggccacgagg
     3361 tcgtggtcgt cgacaacctc gccaacagca gcgcggaagc cctcgagcgc gtgcgcctga
     3421 tcagcggccg cccgctcggg ttccaccggc tggacctgcg cgagggcgcg gcgctgcgcg
     3481 aggtgttcgc gcgcagtcac ttcgacgcgg tgatccactt cgccggtctg aaggccgtcg
     3541 gcgaatcggt gcagaagccc ttgcattact acgacaacaa cgtcaccggg acgctgcggc
     3601 tgctcgagtg catgcgcgag gccgggttga aacggctggt gttcagctcc tcggcgacag
     3661 tctacggcga cccggcatcg gtgccgatac gcgaggactt cgcgctgtcg gcgagcaacc
     3721 cctatgggcg caccaagctg ttcatcgagg agatcctgcg cgacgagcag cgcgccaacc
     3781 cggactggga catcgtgctg ctacgctact tcaacccggt gggcgcgcac gccagcgggc
     3841 tgatcggcga ggatccggcc ggcataccca ataacctgat gccctatgtg agccaggtcg
     3901 ccgtgggcca gctggactgc ctgaacgtct ggggcaacga ttaccccacg cccgacggca
     3961 ccggggtgcg cgactacatc cacgtggtgg acctcgcgct cggacacctc gcggcgctcg
     4021 agcgcgcggc gggaccgggg gtgcgcgcct acaacctggg cacggggcgc ggctattcgg
     4081 tgctggagat gatcgcggcc ttcgagcgcg cctcggggaa caaggtgccg taccgcttcg
     4141 cgccgcggcg acccggcgac gtggccgcct gctacgccga cccggcgctg gcgcgccagc
     4201 aacttggctg gaccgccgag cgggacgtgg acgcgatgtg ccgcgacacg tggcactggc
     4261 agtcgaggaa tccgcgaggc tacggatagt aataccggca acgcaaaaaa cgggggccgg
     4321 tcaagggcac cggcccggtt gcggtatcag cccaaaaaag gagcgccggc gtaaaccggc
     4381 gttcatctct cacaatttcc ggaagacgca gcctccgcga aacccagcgt ctatgctgcc
     4441 accttgcggc gccggaccaa ggacagcccc gcgagacccg caccgaagag cagcatggtg
     4501 ccaggctccg gcgcattgcc gctgggcggt actccgggct gtgtaccggc cagcaaacca
     4561 atcttcatgt agtcgtagtg cgacgtggtg ccatttgtac acccatcacc ggacatctga
     4621 ctgttgtcgt tgcagcctga aaacaccgtg ttataggcca tcacaagcca gtaactcgac
     4681 accaggttgg tgctcagatc cgcagtccag ttgggcctgg tctggagatc ggcatagttg
     4741 cctacgagac tccaaccatt agcgagcaaa ccggtggacg tccctccata tgttctgccg
     4801 acaagtgtcg gatcagtggc cggtttgaca tatgccgcgc cgccgggcgc atccggctgg
     4861 tatgcaagga cagagatgtc tgcatcgttg gaataccagc tcaagccgat atcggtgaga
     4921 ttgatcccct cgctaaactc aaaaagtacc gcatcccacc gaccattgtt atccacgcca
     4981 tgttcagggc tactgccttc gttatagtca cccgagcagc ccgaggtggt gcagcgatcc
     5041 cgattggtca cgccgagacc tccggttggc ggcgcattgc cgtcaccgtc gttccaccag
     5101 gacagataag cggtttcgag cagagtattc gaagtcccgc cggtgttgga gaaagcgctc
     5161 gccgtaaccg ttatgccggc actcgtggcg tcgcgcgtat tgcctgcagt cccgctgctc
     5221 gtgccacaac ccgtcgtaca ggtgaatgcc caactcgtgt ctgcccaaac tggcgcgccg
     5281 gcagcggccg acagcgccgc gagtggagca atgattgcga ttgtccagcg catggtcatt
     5341 tgctcttgtg gttgcgtgtt gcggtttgat cagcaagata tgcgcctaaa agtatttttg
     5401 ccatgtaatc aatgaattat cattgctcgc cgccgcacag ggtgccgcac tgtaaaaatt
     5461 ttcgacaaat tgcctcatcg cgagcccagc cgttgctgca gcttgcgagc gtcctccagt
     5521 tcatcgaact cagccgctgt gctgagtgct tcgcccagtt cggtgcgagc ctcgtcctca
     5581 cgcccgatcg ccgcgagggc ggcggccaga tggtagcgga tggtcggatt ttgcggcgca
     5641 cgcaagcgtg cttcgcgcag gtggcgcagc cctgcctcgg cttgccccga tcccaccagc
     5701 agccagccca gcgtgtcgcg tatcgctgcg tcctgcggcg ccaggtcgtg ggcgcgctgc
     5761 gcgtactcga gcgcgcgcgg gtcgccctgg cgcgccagca cgttcgccag gttgttgagc
     5821 aggtcggggg catcgggctg gtcccgcagc agtgcctcgt aggacgagcg ggcctgcggc
     5881 aggcgccccg cgcgcaggta gccctcggcc agcgcgcgcc gcgcgagggc atcctgtgga
     5941 tgatcccgcg accaggattc catgaattcc accgcgtcgt cctgctgccc caccgcaaaa
     6001 taggcccggt acaggcgcag cgccagttcg ggatccggct ggccgcgcag ggccgactgg
     6061 tagctcgcca cggcctcctc ggccctgccc tgggccaggg cgatgtcgcc gagcaggcgc
     6121 tggcccgtgc tttcggtcgc ctgatcggcc gcgagcgatc gcgcccgctg ctccgcctgc
     6181 tcgaaccggc tcagctgcag ttcgacctcg gtcagcagaa ccagcgccgg gagatggcgc
     6241 ggctcactct ccagcgcccg ctcgaggctg tggatcgccc cctcgggatt gtttgcgctc
     6301 agctggtatt gcgcaatgcg gtgctgccag ccggcgtcga atgccgccag ccgtgtcatg
     6361 cggtcgaaga agacctgcgc ctgcttgtcg tcgcccaggg cgacatgcac cttgccgaga
     6421 gcggctagcg cggccagatc ctccggcgcg acggcgctgg tctctcgcgc caccgtgagc
     6481 gccttctcct tcataccgcc acggagcaga agatcgacga gccgtgcggt ggcaggcacg
     6541 ttacggtgat cattggcgtg cagtttctcc agccagcgca acgcttcggt gtcattcccc
     6601 gccgcctcct cgagcgccgc cagctcgaag gtggcctgcg gatcttccgg gcgtttctgg
     6661 aggatccgct gcaaccggtc ccgcgccgcg gcgagattgc cttcctcgac gtcgaggcgg
     6721 gtcaggttca gctcggcggg gaggaaggcg gcatcgagtg ccaacgcctg caggtacgcg
     6781 gcgcgggccc ccggcttgtc tccgcccgcc tggcgggcga ccccgagcag gttccaccct
     6841 ggcgcgccct ggtcccgttc cgccaccttg cccgccatcg acaccgccgc ttcgaactcg
     6901 cccagcttga tgtacatcac cgtcagcgcg ttgccttttc gagattggga gggctcctac
     6961 atctacactc tttccatgct ggacacgctc gccatctgct gcctcgacgt cgagaaacg
//
LOCUS       DAORUQ010000161         6966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12418_length_6966_cov_5.112864, whole genome shotgun sequence.
ACCESSION   DAORUQ010000161 DAORUQ010000000
VERSION     DAORUQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12418_length_6966_cov_5.112864"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..2387)
                     /locus_tag="PLW13_12585"
     CDS             complement(177..2387)
                     /locus_tag="PLW13_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71262.1"
                     /translation="MRKARGIWFLGAAAAAPVAALAAPAQAPSSVIEEVRIVGQRAEA
                     RQLSGSAHAIDAAELRKFSQTDINRVLRAVPGLYMREEEGYGLRPNIGIRGSGAERSS
                     KISLMEDGVLIAPAPYADPAAYYFPTAGRMSAIEVLKGPETMRYGPFTVGGAMNMVST
                     PIPRQAGGRVLAEVGGNGEQRVMGNYGAGAEQYGWLLETVQHQADGFHDIDRSGKDTG
                     FEIEDYVAKARLNSAEGARFHQQLDVKLQYSEETSQASYLGLSDEDFDRDANRRYGLS
                     ALDEMTNDHQGYSARYRFEFSEQLGLNLLWYRNEFTRDWFKLDRVNAQSMSGMISAIN
                     AGAATAPLYQGWLDGTLDVNNVEIKHNNREYEAEGLQFTLDARFATGAWEHELEFGAR
                     RHEDEIDRYQPVERYNQVGGQLVYASTLAPNLASNNAVGTGDADSFWLLDRITHGALT
                     LVPLIRVEDIETRNRQWSNDPPRAGAASVKGNRLGNETSLGLGATWDIDGHWQLLAGV
                     HEGFAPPGADSVDGTEAEESLNYEGGVRYTDERYSADAIFFYSDYTNTIKNCSVANPC
                     TGGQTVGTESLGESEIQGVEIGLNTGFDGPAGTTWPLRFSYTWTDAEVTEDSETGNVL
                     EGDTLADIPEQVAALSLGVVHPSGWDSYLNASYYDQTCSTYTCDRAGVDDRFLRTDDL
                     FLVDWTAGYRLFEGLRVYAKLNNVFDEQVIVSRKPDGARSNMPRTAYLGADWSF"
     gene            complement(2507..4843)
                     /locus_tag="PLW13_12590"
     CDS             complement(2507..4843)
                     /locus_tag="PLW13_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQY71263.1"
                     /translation="MLSIQERIAAELNVGVSQVAAAVGLLDDGATVPFVARYRKEVTG
                     GLDDVQLRALEQRLAYLRELEDRREAIVKSITEQGKMTPALETSIREADSKARLEDLY
                     LPYKPKRRTKGQIAREAGIEPLADLLLTDPMRDPATEAVAFLNAEAGFADTKAVLDGA
                     RHILMERFAEDAELVGRVRQLLREQGQLQARVADGKEAEGAKFADYFAHAEPLAKVPS
                     HRALAMFRGRNEGVLYLAVDLPGAAETSAHPCEGMVAAHAGISDKGRPADAWLMEVAR
                     WTWRVRLSVHVVGELLTELREAAEAEAIRVFAANLRDLLLAAPAGHRACIGLDPGLRT
                     GVKVVALDGTGKLLEYTTIYPHVPQNKWRQSLDTLAALARKHGSDLIAIGNGTASRET
                     EKLAAELIAAHPELGLTRVVVSEAGASVYSASELASQEFPDIDVSIRGAISIGRRLQD
                     PLAELVKIEPKSIGVGQYQHDLNQLRLSKSLDAVVEDCVNSVGVELNSASAALLSRVS
                     GLGGSLAQAIVAHRDQHGPFRSREALRQVPRLGARTFEQCAGFLRIRLGDNPLDASAV
                     HPESYPVVERIAAAQGCSVSELIGNAERVRGLDPCRFVTAEAGELTVRDILRELEKPG
                     RDPRPAFKTATFKEGVEELKDLQPGMVLEGSVTNVANFGAFVDIGVHQDGLVHVSALS
                     NRFVKDPREVVKAGDVVKVKVLEVDIARKRIALTMRLDDEVPAEGAPRQKGGGARPQP
                     GRANEQRQHQPRRDEPRATAPKTGTLGELLLAAQKRQR"
     gene            5074..5373
                     /locus_tag="PLW13_12595"
     CDS             5074..5373
                     /locus_tag="PLW13_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQY71264.1"
                     /translation="MEYDDSNKIFFGRLAGMRDVVSFHGEFVAELEAAFHEAVDDYVA
                     ACAKLGRTPEKPASGRLMLRIPPEVHGAALVAAQAAGTSLNQWAAKVLAEAAHVD"
     gene            complement(5664..5740)
                     /locus_tag="PLW13_12600"
     tRNA            complement(5664..5740)
                     /locus_tag="PLW13_12600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5704..5706),aa:Arg,seq:acg)
     gene            5859..6515
                     /locus_tag="PLW13_12605"
     CDS             5859..6515
                     /locus_tag="PLW13_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQY71265.1"
                     /translation="MNTRKALLWDLPVRLFHWSLPLLVVTAWISAEYDAMDVHEWCGY
                     TVLVLVGFRIVWGFIVSRHARFSDFLRSPAAALRYLRGLEPERPGHNPAGGWSVIAML
                     TLLAAQGVTGLFNSDDVSFEGPLVRALDRGSVDALAAWHEPNFWLLLALVALHIGTVL
                     FYLLRRQKNLIAPMLNGGESSAAGTPRPLWLALVVIAVLSGLLYWGLSLAPPPPAPFL
                     "
     gene            complement(6708..>6966)
                     /locus_tag="PLW13_12610"
     CDS             complement(6708..>6966)
                     /locus_tag="PLW13_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQY71266.1"
                     /translation="ELAALAGLNQMRGFPEGSDKGDTHAKPEIWKNMDDFEKKMEEFQ
                     LAAAKLGKVAAGGDRKAISTEIEATGKTCGACHDDYKEKDD"
BASE COUNT         1044 a         2378 c         2316 g         1228 t
ORIGIN      
        1 gcctggcgcg actacttcga cctggcccag ttccagaacg gcatggcggg ctgagcggcc
       61 cgcgcagcaa accgaaacac cgtgggtccg ctttcaagcg gactttgtcg ggctgaagcc
      121 cgacctacgg cggactttgt ccggatgaat ccggacccac cgggtgggag gtatcgtcag
      181 aagctccagt cggcgccgag gtaggccgtg cgcggcatgt tggagcgcgc cccatcgggc
      241 ttgcgcgaca cgatcacctg ttcgtcgaag acgttgttga gcttcgcata gacgcgcagc
      301 ccctcgaaca ggcggtagcc ggccgtccag tcgaccagga acagatcgtc ggtgcgcagg
      361 aagcggtcat cgacgccggc acggtcgcag gtgtaggtcg agcaggtctg gtcgtagtac
      421 gaggcgttca ggtagctgtc ccagccggac ggatgcacca cgcccaggct gagcgcggcc
      481 acctgctcgg ggatatccgc cagggtatcg ccctccagca cgttgccggt ctcggaatcc
      541 tccgtcacct cggcgtcggt ccaggtgtag gagaatcgca gcggccacgt ggtccccgcc
      601 ggaccgtcga acccggtgtt caggccgatc tcgacgccct ggatctccga ctcgccgagg
      661 ctctcggtcc cgacggtctg gccgcccgtg cagggattgg ccactgagca gttcttgatg
      721 gtgttggtgt agtcgctgta gaaaaagatc gcgtcggcgc tgtagcgctc gtcggtgtag
      781 cgcacgccgc cctcgtagtt gaggctctcc tcggcctcgg tgccgtcaac cgaatcggcc
      841 cccggcggcg cgaagccctc gtgcacaccg gcgagcaact gccagtggcc gtcgatatcc
      901 caggtggcgc ccagtcccag gctggtctcg ttgccgaggc ggttgccctt cacggacgct
      961 gcgccggcgc gcggcggatc gttgctccac tggcggttgc gcgtctcgat gtcctcgacg
     1021 cgtatcaatg gcaccagcgt cagtgcgccg tgcgtgatgc gatccagcag ccagaaggaa
     1081 tcggcatcgc cggtgcccac ggcattgttg gatgccaagt tcggcgcgag cgtcgaggcg
     1141 tagaccagct gtccgccaac ctggttgtaa cgctccaccg gctggtagcg gtcgatctcg
     1201 tcctcgtgac gccgcgcgcc gaactccagc tcgtgctccc aggcgccggt ggcgaaacgg
     1261 gcatccagcg tgaactgcag gccctcggcc tcgtactcgc ggttgttgtg cttgatctcg
     1321 acgttgttca cgtccagcgt gccatcgagc cagccctggt agagaggcgc cgtcgccgcg
     1381 ccggcgttga tggcggaaat catcccgctc atcgactgcg cgttcacgcg gtcgagcttg
     1441 aaccagtcgc gcgtgaactc gttgcgatac cacaggaggt tcagccccag ctgttcgctg
     1501 aactcgaagc ggtagcgcgc actgtagccc tggtgatcgt tcgtcatctc gtcgagcgcc
     1561 gagagcccgt agcggcggtt cgcgtcgcga tcgaaatcct cgtcgctgag ccccaggtag
     1621 gaggcctgcg aggtctcctc ggagtactgc agcttcacgt ccagctgctg atggaagcgc
     1681 gcgccctcgg ccgagttcag ccgcgccttg gcgacgtagt cctcgatctc gaaaccggtg
     1741 tccttgccgc tgcggtcgat gtcatgaaaa ccgtcggcct ggtgctgcac cgtctccagc
     1801 aaccagccgt actgctcggc gccggcgccg tagttgccca tcacgcgctg ttcgccgttg
     1861 ccaccgactt cggcgagcac gcgcccgcct gcctggcgcg ggatcggggt ggacaccatg
     1921 ttcatcgcgc cgccgaccgt gaacgggccg tagcgcatgg tttcggggcc cttcagcacc
     1981 tcgatggcgg acatgcggcc cgccgtgggg aagtaatacg cggcgggatc cgcgtaggga
     2041 gccggggcga tcagcacgcc gtcttccatc aggctgatct tgctgctgcg ctcggcaccg
     2101 gaaccgcgga tgccgatgtt cggccgcagg ccgtagccct cctcttcgcg catgtacagg
     2161 ccgggcacgg cgcgcagcac ccggttgatg tcggtctggg agaacttcct gagctccgcg
     2221 gcatcgatcg cgtgggccga gccgctcagc tggcgtgcct cggcacgctg acccacgatg
     2281 cggacttcct cgatcaccga tgagggcgct tgcgccggcg cggcgagcgc tgccacggga
     2341 gcggccgccg ccgcgcccag gaaccaaata cccctggcct tgcgcatcgt gatgtctccc
     2401 cttgttcgcg attcgtgtcc ggcatcacgc cggtacggaa agggagacta gcagattgaa
     2461 tctaaatgcg aaacattatt gtttagatta gtagctcgat ggcccgtcag cgctggcgct
     2521 tttgcgccgc cagcagcaac tcgcccagcg tgccggtctt cggcgcagtg gcgcgcggct
     2581 cgtcgcggcg cggctggtgc tgacgctgct cgttcgctcg ccccggctgc gggcgcgccc
     2641 cgccaccctt ttgccgcggc gcgccctcgg ccggcacctc gtcgtcgagg cgcatggtca
     2701 gggcgatgcg cttgcgcgcg atgtcgacct ccagcacctt caccttgacc acgtcgccgg
     2761 ccttcaccac ctcgcgcgga tccttcacga aacggttgct gagcgccgag acgtgcacca
     2821 gaccgtcctg gtgcacgccg atgtcgacga aggcgccgaa gttcgcgacg ttggtcacgg
     2881 agccttcgag caccatgccc ggctgcaggt ccttcagttc ctcgacgccc tccttgaatg
     2941 tcgcggtctt gaaggccggg cgcgggtcgc gcccgggctt ctccagctcg cgcaggatgt
     3001 cgcgcaccgt gagctcgccg gcttcggcag tcacgaaccg gcagggatcg aggccacgca
     3061 cgcgctcggc gttgccgatc agttccgaca cggagcaacc ctgcgccgcc gcgatgcgct
     3121 cgaccaccgg gtaggactcc gggtgcaccg ccgaggcatc gagcgggttg tcgccgagcc
     3181 ggatgcgcag gaagcccgcg cactgttcga aggtacgcgc gccgaggcgc ggcacctggc
     3241 gcagcgcctc gcgcgagcgg aacggcccgt gctggtcgcg gtgcgccacg atggcctgcg
     3301 ccagcgagcc gcccaggccc gagacgcggc tcagcagcgc cgccgaggca ctgttcagtt
     3361 ccacgcccac gctgttcacg cagtcctcga ccacggcgtc gaggctcttc gacagcctca
     3421 gctggttcag gtcgtgctgg tactggccga caccgatcga tttgggttcg atcttgacca
     3481 actcggccag cggatcctgc aggcgacggc cgatcgagat cgcgccgcgg atgctgacat
     3541 cgatgtcggg gaattcctgc gaggccagct cggaggccga gtacaccgag gcgccggcct
     3601 cgctgaccac cacgcgcgtg aggccgagct cgggatgtgc cgcgatgagc tccgcggcca
     3661 gcttctcggt ttcgcgcgag gcggtgccgt tgcctatcgc gatcaggtcg ctgccgtgct
     3721 tgcgcgcgag cgccgccagc gtgtcgagcg actggcgcca cttgttctgc ggcacgtgcg
     3781 gatagatggt ggtgtattcg agcagcttgc cggtgccgtc gagcgccacc accttcacgc
     3841 cggtacgcag tcccggatcc agtccgatgc aggcgcgatg ccccgccggc gcggccagca
     3901 gcaggtcgcg caggttggcc gcgaacacgc ggatcgcctc ggcctcggcc gcctcgcgca
     3961 gctccgtgag cagttcgccc accacgtgca ccgagaggcg cacgcgccag gtccagcgcg
     4021 ccacctccat cagccaggcg tctgccgggc ggcccttgtc actgatgccg gcgtgcgcgg
     4081 ccaccatccc ctcgcagggg tgcgcgctcg tctcggcggc gcccggcagg tccacggcca
     4141 ggtagagcac gccctcgttg cgcccgcgga acatcgccag cgcacggtgc gaaggcacct
     4201 tggccagcgg ctcggcatgc gcgaagtaat cggcgaactt ggcgccctcg gcctccttgc
     4261 catcggccac gcgcgcctgc agttgccctt gctcgcgcag cagctggcgc acgcgcccca
     4321 cgagctcggc gtcctcggcg aagcgttcca tcaggatgtg acgcgcgccg tcgagcacgg
     4381 ccttggtgtc ggcgaaaccc gcctcggcgt tcaggaacgc caccgcctcg gtggccggat
     4441 cgcgcatggg atcggtgagc agcagatccg cgagcggctc gatacccgcc tcgcgcgcga
     4501 tctggccctt ggtgcggcgc ttgggcttgt acgggaggta gagatcctcg aggcgcgcct
     4561 tggaatcggc ctcgcggatc gaggtttcca gcgccggcgt catcttgccc tgctcggtga
     4621 tgcttttcac gatcgcctcg cggcgatcct ccagctcgcg caggtaagcg agacgctgct
     4681 ccagcgcgcg cagctgcacg tcatcgagcc cgccggtcac ttccttgcga tagcgcgcca
     4741 cgaagggcac cgtggccccg tcgtcgagca gcccgaccgc cgcggcgacc tggctgacgc
     4801 cgacgttcag ttccgcggca atgcgttctt gaatcgacaa catgcgggtg actccatcct
     4861 gcgaggcggc ggattatgcg caccgccgga cccggccgcg agacttgtgg aagccggcgc
     4921 ttttgggtag ggacgaatga catcgcgtcg ggtgcgcatc gctgatgccg gcgaggaggc
     4981 gctgcgatat cgcgtcatcg cggcgcgcga gtttctcgaa aagatcggaa tccaaccatg
     5041 aacgccatga cctactaggg ctataccgcc cgcatcgaat acgacgacag caacaagatt
     5101 ttctttggcc ggctggccgg gatgcgcgac gtcgtatcgt tccacggcga gttcgttgcc
     5161 gagctcgaag cggcgtttca cgaggccgtc gatgattatg tcgccgcctg tgcgaaactc
     5221 ggccggacgc ccgagaagcc cgccagcggc agattgatgc tgcgcatccc gccggaggtg
     5281 catggcgcag cgctggtggc ggcgcaagcg gccggaacga gcctcaacca atgggcggcg
     5341 aaggtgttgg ccgaagcggc tcacgtcgat tgaccatcag cagatgagct cgcccgtcga
     5401 ggacgcgaag gccttgcaca acagcccggc ctgccagccc gccattcccg tgggtcggca
     5461 tgcatggcga cgacagcgcg cggcacggct aggcggagag attgactcgc gccatccatg
     5521 gcgctcgccc ctgcggggcg cctacgcttc gctgcggcgt ccaaatcggc agtccctgcc
     5581 gatttgtcga actggggagt tctctgttcc actctccacg cacgccaaac gaaaaagccc
     5641 agcgcaagct gggctttttc gtttggcgca cccggagaga ttcgaactcc cgacccccta
     5701 gttcgtagcc aggtactcta tccaactgag ctacgggtgc gaaggccgcg tactatattg
     5761 agccggtccg gcttcgtcaa cctcgatgga aagcgaagtt ttacccatcg ctacggacta
     5821 gaattcgcgg caagtccgtg accggagaag tgacgccgat gaacacccgc aaagccctgc
     5881 tatgggacct cccggtgcgc ctgttccact ggtcgctgcc tctgctggtg gtaacggcgt
     5941 ggatcagcgc ggaatacgac gcgatggacg tgcacgagtg gtgcggctac acggtgctgg
     6001 tgctggtggg ttttcgcatc gtgtggggtt tcatcgttag ccggcacgcg cgcttcagtg
     6061 acttcctgcg ctcgccagcg gcggcgctcc gctacctgcg cgggcttgaa cccgagcgcc
     6121 ccgggcacaa cccggccggc ggctggtctg tcatcgcgat gctgacgctg ctcgcggcgc
     6181 agggcgtgac cgggttgttc aactccgatg acgtcagctt cgagggaccg ctggtgcggg
     6241 cgctggatcg tggcagcgtc gatgcgctgg cggcatggca cgagccgaac ttctggctgc
     6301 tgctcgcgct ggtggcgctg cacatcggca cggtgctgtt ctacctcctg cgcaggcaga
     6361 agaacctgat cgcgccgatg ctgaacggag gcgagtcttc cgccgcaggc acgccgcgcc
     6421 cgctgtggct ggccctcgtg gtgatcgccg tgctttcggg gctgctgtac tgggggctgt
     6481 cgctggcgcc accgccgcca gcgccgttcc tctgagaacg ccgaaaaacg ccatctgcgc
     6541 ccgcccccgc aggtgcgggt tcagccgcac aactgtccgc atgaatgcgg acctacgggt
     6601 attgctcccc acgtgcgcga ccatcgcgcg catggcgcgc tcctacagcc agcggtcatg
     6661 tcgtggcggg gctgccccgc cacacgcgac tctgcgcggc gagggactca gtcgtccttt
     6721 tccttgtagt cgtcgtggca ggcaccgcag gtcttgccgg tcgcctcgat ctcggtcgag
     6781 atggccttgc gatcgccgcc cgccgccact ttgcccagct tcgcggccgc cagctggaac
     6841 tcctccatct tcttctcgaa gtcgtccatg ttcttccaga tctcgggctt ggcgtgcgtg
     6901 tcgcccttgt cggagccctc cgggaatccg cgcatctggt tgagccccgc gagggcggcg
     6961 agttcc
//
LOCUS       DAORUQ010000162         6954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12453_length_6954_cov_6.269314, whole genome shotgun sequence.
ACCESSION   DAORUQ010000162 DAORUQ010000000
VERSION     DAORUQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12453_length_6954_cov_6.269314"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLW13_12615"
     CDS             complement(<1..410)
                     /locus_tag="PLW13_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY71267.1"
                     /translation="MHKQRLLPSAALAALLGLAALGAQATERVYKWVDAEGVVHYGQQ
                     PPATAKAEAIKVQKGYSTSDPDAPVDLTDEQKKAAADAETCRTATENHKALSGAGEVT
                     RVDEYGEKHTMSEEEKNAEAARAKAAMERFCKPS"
     gene            839..2587
                     /locus_tag="PLW13_12620"
     CDS             839..2587
                     /locus_tag="PLW13_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HQY71268.1"
                     /translation="MELLSGGEMVVQALEDEGVEYVFGYPGGAVLHIYDALFKHGGKL
                     THVLVRHEQAATHAADGYSRSTGKPGVVLVTSGPGATNAITGIATAYMDSIPMVVISG
                     QVPRDKIGEDAFQETDMVGISRPIVKHSFMVTQAEQIPETIKKAFHIATTGRPGPVVV
                     DIPKDVTSPAEKFEYAYPERVRMRSYNPVTRGHGGQIRKAVQLLLTAKRPVIYSGGGV
                     ILGNGSAQLRKLAEVLNYPVTSTLMGLGAYPASSPRFLGMLGMHGTYEANMAMHHSDV
                     IIAIGARFDDRVTNTPNRFCPDAKIIHVDVDPTSISKTIVADLPITGPVDVVLDEMLS
                     QIEDVQRSAQGGPDRAALANWWNEIERWREAHGIYRKPRYDSSGPLIMPQDVIKCLHE
                     VTAGNAFVTSDVGQHQMFAAQYYLFEEPRRWINSGGLGTMGFGLPAAMGVKLAHPDAD
                     VACVTGEGSIQMCIQELSTCTHYSLPVKIINMNNGYLGMVKQWQDMQYAGRHSHSTYQ
                     DSLPDFVKLAEAYGHVGMRVESKEELRPAMEKAFALKDRLVFMDIVVDPHEHVYPMHI
                     APGSLRDMWLSKTERT"
     gene            2589..3080
                     /gene="ilvN"
                     /locus_tag="PLW13_12625"
     CDS             2589..3080
                     /gene="ilvN"
                     /locus_tag="PLW13_12625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQY71269.1"
                     /translation="MRRIISLLMENEAGALSRVIGLFSQRGFNIETLNVAPTEDPTLS
                     RLTLTTKGDDRVIEQITKQLNKLVDVVKLSDLTEGAHIERELMLIKLRATGAQRDEIK
                     RCADIFRGQIVDVSSSLYTVLLTGTQQKLDAFIQAVGDAVILEVVRSGVAGIARGDRV
                     LSL"
     assembly_gap    3101..3200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3301..4776)
                     /gene="ilvC"
                     /locus_tag="PLW13_12630"
     CDS             complement(3301..4776)
                     /gene="ilvC"
                     /locus_tag="PLW13_12630"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQY71270.1"
                     /translation="MSDNYFNTLPLRLQLEQLGKCRFMDRSEFAHETQALRGKKVVIV
                     GCGAQGLNQGLNMRDSGLDVSYTLRQSAIAEKRQSWKNATENGFKVGSYEELIPTADL
                     VCNLTPDKQHTAVVKEIMPLMKQGACLAYSHGFNIVEEGMQIRPDLTVIMVAPKCPGT
                     EVREEYKRGFGVPTLIAVHPENDPRGEGLELAKAYAAGTGGHRAGVLESSFIAEVKSD
                     LMGEQTILCGMLQAGSLLCFDKMVEKGIPKAYACKLVQYGWETVTEALKHGGITNMMD
                     RLSNPAKIAAFELAEQLKGIMRPLFNKHMDDIMSGEFSRTMMADWAADDRNLLGWREA
                     TGKTAFEQANADPAATISEQQYFDQGILMVAMIKAGVELAFETMVSSGIIEESAYYES
                     LHETPLIANLIARKKLYEMNKVISDTAEYGCYLFAHAAVPMLADFMRDIDTDVIGKGL
                     AGDHGVDNQELIAVNAAIRNHPVEIVGRALRSHMTAMKKIV"
     gene            4897..5790
                     /gene="ilvY"
                     /locus_tag="PLW13_12635"
     CDS             4897..5790
                     /gene="ilvY"
                     /locus_tag="PLW13_12635"
                     /inference="COORDINATES: protein motif:HMM:NF008722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HQY71271.1"
                     /translation="MDSRDLEIFLLLAETLHFGRAAEHGNLSPSALSRTIQRLESELD
                     CRLFDRGNREVRLTRQGRLFQAQARDLLERMRDLRRRVRADSAALQGSVTLYCSVTAS
                     YSLLAGLLPEFRARYPGVEINVHTGDEASALRRVAQGREDLAIAARPDQLPANIAFLE
                     LATTPLVFIAPASSQFERLPGATPGLDWSLLPMILPETGQARERVDHWFADMGVAPNV
                     YAQVSGNEAIVSMVALGCGVGVVPQLVLKSNPFGDSVHVLEVMPELHPFRIGLCCRQA
                     SLANPLVGALWGLAGEGLRRG"
     gene            5846..6676
                     /gene="pssA"
                     /locus_tag="PLW13_12640"
     CDS             5846..6676
                     /gene="pssA"
                     /locus_tag="PLW13_12640"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQY71272.1"
                     /translation="MDQDADKSGEDTQEIELGLPVDEHIEEVSVGGLRERRRGVYLLP
                     NLLTTGALFAGFYAIVASMNAQFEAASLAIFVAMFFDGLDGRVARLTNTQSAFGVQYD
                     SLSDMVSFGVAPALVAFSWALSSMGKLGWAAAFVYVAGAALRLARFNTQAATSDNSFF
                     RGLASPAAAALLASLVWSATDLGFQGDELPSVIAALATFVTAGAGLLMVSNFRYQSFK
                     KFDLRRRVPFVIMLLMVLVVGVVTVDPPRILLLVAVVYALSGPGQLAVQRLRMRRNAP
                     "
     gene            complement(6754..>6954)
                     /locus_tag="PLW13_12645"
     CDS             complement(6754..>6954)
                     /locus_tag="PLW13_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71273.1"
                     /translation="LLDLFFLNNCGYASVPPPLLGLSPSGKAHYTDRFRRGKHRGKKV
                     RLYFSRLGIGRFSLTAGPPRAR"
BASE COUNT         1119 a         2167 c         2280 g         1288 t
ORIGIN      
        1 tcggaaggct tgcagaagcg ctccatggcg gccttggcac gcgcggcctc ggcgttcttt
       61 tcttcctcgc tcatcgtgtg cttctcgccg tattcgtcga cgcgggtcac ctcgccggcg
      121 ccagagagcg ccttgtggtt ctcggtggcc gtgcggcagg tctcggcgtc ggcggccgcc
      181 ttcttctgtt cgtcggtcag gtcgacgggc gcatccggat cgctggtgga atagcccttc
      241 tgcaccttga tggcctcggc cttggctgtg gccgggggct gctgcccgta atgcaccacg
      301 ccctcggcgt ccacccactt gtagacgcgt tcggtggcct gcgcgcccag tgccgcgagg
      361 cccaggaggg ccgccagcgc cgcgctcggc agtaatcgtt gcttgtgcat gggtcgactt
      421 ccgcaaggtg gccacagttc ggctccatta tagtcagtgc gcgccgaatc gaccgccggc
      481 gccgcgacgg tgcggccaaa gcgtgcggtt gcgcaatatt gcggttgact tgtttgcagt
      541 gcgccgggaa gaatgaacgc tccccggggc cttgccgtga tctcacccgt gatcggcggg
      601 gccaggaaag acgaaaacag cgtgtttccg cccccggcat tcagacgaca ggcgcttaat
      661 caactgcttt gctccgggat cacaggcatc atcaggctgg cgctccctgg catctgatca
      721 gaattcagca aaggacttgt cgtggccggc ccctgcgggg tgcgatgttg ccgcggcatc
      781 actgcgattc atggcgaacg gaagcgcgcg gcgtgctcaa ctcacggagg tgatctacgt
      841 ggaactgttg tctggcggtg aaatggtggt acaggcgctc gaggacgagg gcgtcgagta
      901 cgtcttcggt tacccgggtg gagccgtcct tcacatctac gacgccttgt tcaagcacgg
      961 cggcaagctg acgcacgtgc tggtgcgcca cgagcaggct gccacgcacg cggccgacgg
     1021 ctactcgcgc agcaccggca aaccgggcgt ggtgctggtg acctcggggc cgggggccac
     1081 gaacgcgatc accggcatcg ccactgccta catggactcg atccccatgg tggtgatttc
     1141 cgggcaggtg ccgcgcgaca agatcggcga ggacgccttc caggaaaccg acatggtcgg
     1201 catctcgcga ccgatcgtga agcacagctt catggtcacg caggccgagc agattcccga
     1261 gacgatcaag aaggcgttcc atatcgctac caccggccgc ccgggcccgg tggtcgtcga
     1321 catcccgaag gacgtcacca gtcccgcgga gaagttcgag tacgcctacc ccgaacgcgt
     1381 acgcatgcgt tcctacaacc cggtgacccg cggtcacggc ggccagatcc gcaaggccgt
     1441 gcagctgctg ctgacggcga agcgtccggt catctacagc ggcggcgggg tgattctcgg
     1501 caacggctcg gcgcagctgc gcaagctcgc cgaggtgctg aactacccgg tcaccagcac
     1561 gctgatgggg ctgggcgcgt acccggccag cagcccgcgc ttcctcggca tgctcgggat
     1621 gcacggcacc tacgaggcca acatggcgat gcatcacagc gacgtgatca tcgcgatcgg
     1681 tgcgcgtttc gatgatcgcg tcaccaacac gccgaaccgt ttctgtcccg atgcgaagat
     1741 catccacgtc gatgtggacc cgacctcgat ctccaagacc atcgtggccg acctgccgat
     1801 caccggcccg gtcgatgtgg tgctcgacga gatgctctcg cagatcgagg acgtgcagcg
     1861 ctcggcgcag ggcggccccg atcgcgccgc gctggccaac tggtggaacg agatcgagcg
     1921 ctggcgcgag gcgcacggca tctatcgcaa gccgcgctac gacagcagcg gcccgctgat
     1981 catgccgcag gacgtgatca agtgcctgca cgaggtcacc gcgggcaatg ccttcgtgac
     2041 ctccgacgtg ggtcagcacc agatgttcgc ggcgcagtac tacctcttcg aggagccgcg
     2101 gcgctggatc aactccggcg ggctgggcac catgggcttc ggcctgccgg cggcgatggg
     2161 cgtgaagctc gcgcacccgg acgccgacgt ggcctgcgtg accggtgagg gcagcatcca
     2221 gatgtgcatc caggagctct cgacctgcac gcactactcg ctgccggtga agatcatcaa
     2281 catgaacaac ggctacctcg gcatggtcaa gcaatggcag gacatgcagt acgccgggcg
     2341 gcactcgcac agcacctacc aggattcgtt gccggatttc gtgaagctcg ccgaggccta
     2401 tggccacgtc ggcatgcgcg tcgagtcgaa ggaggaactg cgtcccgcga tggagaaggc
     2461 gttcgcgctg aaggatcgcc tggtgttcat ggacatcgtg gtggacccgc acgaacacgt
     2521 gtacccgatg cacatcgcgc ccggctccct gcgggatatg tggctcagca agacggagag
     2581 gacctgagat gcgccggatc atttccctgt tgatggagaa cgaggcgggt gccctgtcgc
     2641 gcgtgatcgg gcttttctcg cagcgcggtt tcaacatcga gacgctgaat gtcgcgccga
     2701 ccgaggatcc cacgctgtcg cgtctcacgc tgacgacaaa gggcgacgat cgcgtcatcg
     2761 agcagatcac caagcagctg aacaagctgg tcgacgtggt gaaactgagc gatctcaccg
     2821 aaggcgcgca catcgagcgc gagctgatgc tgatcaagct gcgcgcgacc ggcgcgcagc
     2881 gagacgagat caagcgctgc gcggacatct tccgcggcca gatcgtcgat gtgagcagct
     2941 cgctctacac ggtcctgctg acgggcacgc agcagaagct cgacgccttc atccaggcgg
     3001 tgggcgatgc ggtgattctg gaagtggtgc gcagcggtgt cgccggcatt gcgcgcgggg
     3061 accgggtgct gagcctgtag gtcgggctcg taggtcgggc nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn tccggcgaag tcgagccctc tacggtcgct gtcgccatga
     3241 atggcaaccc acatcaaggg caggcgcgcc cggagccctc cgggcgcgcg ccgctcttca
     3301 tcagacgatt ttcttcatcg cggtcatgtg cgagcgcagc gcgcgaccta cgatctcgac
     3361 cgggtggttg cggatcgccg cgttcaccgc gatcagctcc tggttgtcga cgccgtggtc
     3421 acccgccagg cccttgccga tcacgtcggt gtcgatgtcg cgcatgaagt ccgcgagcat
     3481 cggcacggcg gcgtgcgcga acaggtagca gccgtattcc gccgtgtccg aaatgacctt
     3541 gttcatttcg taaagcttct tgcgcgcgat caggttggcg atcagcggcg tctcgtgcag
     3601 cgattcgtag taggccgatt cctcgatgat gcccgaggac accatggtct cgaacgccag
     3661 ctccacgccg gccttgatca tcgcgaccat caggatgccc tggtcgaagt actgctgttc
     3721 ggaaatggtc gccgccggat cggcgttcgc ctgttcgaag gcggtcttgc cggtggcctc
     3781 gcgccagccg agcaggttgc ggtcgtcggc ggcccagtcg gccatcatgg tgcgcgagaa
     3841 ttcgccgctc atgatgtcgt ccatgtgctt gttgaacagc gggcgcatga tgcccttcag
     3901 ctgctcggcg agctcgaagg cggcgatttt cgccgggttc gacagccggt ccatcatgtt
     3961 ggtgatgccg ccgtgcttca gcgcttccgt gacggtttcc cagccgtact gcaccagctt
     4021 gcacgcgtag gccttcggga tgcccttctc gaccatcttg tcgaagcaca gcagcgaacc
     4081 ggcctgcagc atgccgcaga ggatcgtctg ttcgcccatg aggtcggact tcacttcggc
     4141 gatgaacgag gattccagca cgccggcgcg atggccgccg gtgccggccg cataggcctt
     4201 ggccagctcg aggccttcgc cgcgcggatc gttctccggg tgcaccgcga tcagcgtcgg
     4261 cacgccaaag ccgcgcttgt attcctcgcg cacctcggtg ccggggcact tcggcgcgac
     4321 catgatcacg gtcaggtcgg gacggatctg catgccttcc tcgacgatgt tgaaaccgtg
     4381 cgagtacgcg aggcacgcgc cctgtttcat cagcggcatg atttccttca ccaccgcggt
     4441 gtgctgcttg tcgggcgtca ggttgcacac gaggtcggcg gtggggatca gttcctcgta
     4501 gctgccgacc ttgaagccgt tctcggtggc gttcttccat gactggcgct tttccgcgat
     4561 ggcgctctgg cgcagcgtgt agctcacgtc cagtccgctg tcgcgcatgt tgaggccctg
     4621 gttcaggccc tgggcgccgc agcccacgat cacgaccttc ttgccgcgca gtgcctgggt
     4681 ctcgtgcgcg aattcgctgc ggtccatgaa gcggcacttg ccgagctgct cgagttgcag
     4741 gcgcagcggc agcgtattga aataattgtc ggacatgatg gggctcctgt gatgcgccgc
     4801 gcccggggcg gggcggtcga gggggcacga tacgtcggcg ctcgtgttgc gtaaagtgat
     4861 atattgagaa cgttatattg cggaaaacgc aacgatatgg attcgcgtga cctcgagata
     4921 ttcctgctcc tcgccgaaac gctgcacttt ggccgggccg ccgagcacgg caacctgtcg
     4981 ccctcggcgc tgagtcgtac catccagcgc ctcgagtccg aactggattg ccggctgttc
     5041 gaccgcggca atcgcgaggt gcgcctcacg cggcagggtc ggctgttcca ggcacaggcg
     5101 agggatctgc tcgagcgcat gcgcgacctg cgccgccggg tgcgcgcgga ttccgcggca
     5161 ttgcagggca gcgtgacgct ctattgttcc gtgacggcga gctacagcct gctcgcgggc
     5221 ctgctgccgg aattccgcgc ccgctacccg ggtgtggaga tcaacgtgca cacaggtgac
     5281 gaggccagcg cgctgcggcg cgtggcgcag gggcgcgagg atctcgcgat cgccgcgcgc
     5341 cccgaccagt tgccggccaa tatcgccttc ctcgagctgg ccacgacgcc gctggtgttc
     5401 atcgcgcccg cctccagcca gttcgagcgc ctccccggcg cgacgccggg gctcgactgg
     5461 agcctgctgc ccatgatcct gcccgaaacg gggcaggcga gggagcgggt ggatcactgg
     5521 tttgccgaca tgggggtggc gccgaatgtc tacgcgcagg tctccggcaa cgaggccatc
     5581 gtcagcatgg tggcgctcgg ctgcggtgtc ggcgtggtgc cccagctggt actgaaatcg
     5641 aaccccttcg gcgacagcgt tcacgtgctc gaagtgatgc cggaactgca tccgttccgg
     5701 atcgggctgt gttgccgcca ggcatcgctg gccaacccgc tggtaggtgc gctgtggggg
     5761 ctggccggcg agggtctgcg tcgcggctga cacccccgtc gacgctgcct tatagtagaa
     5821 gcaattcatg cgaaggagtc attcggtgga ccaggacgca gacaagagcg gcgaggacac
     5881 ccaggaaatc gaactcgggt tgccggtcga cgagcacatc gaggaagtat ccgtgggagg
     5941 gctccgggag cgccggcgtg gcgtgtacct gttgccgaac ctgctgacga cgggggcgct
     6001 ctttgccggc ttctatgcaa tcgtcgcgag catgaacgcg cagttcgagg ccgcttcgct
     6061 cgcgatcttc gtcgcgatgt tcttcgacgg actcgacggg cgcgttgcgc gcctgaccaa
     6121 cacgcagagc gccttcggcg tgcagtacga cagcctgtcg gacatggtct cctttggcgt
     6181 ggcgcctgcg ctggtcgcgt tcagctgggc gctctcgtcg atgggcaagc tgggctgggc
     6241 cgcagcattc gtgtacgtgg ccggcgcggc gttgcggctg gcgcgattca atacccaggc
     6301 cgccacatcc gacaacagct tttttcgcgg actcgccagc cccgcggcgg cggcgttgct
     6361 ggcctcgctg gtgtggtccg ccaccgatct gggattccag ggcgatgagt tgcccagcgt
     6421 gatcgcggca ctggcgacat tcgtcacagc gggcgcgggc ttgctgatgg tcagcaattt
     6481 ccgttaccag agtttcaaga agttcgacct gcgccggcgt gttcccttcg tgatcatgct
     6541 gctgatggtg ctcgtcgtgg gtgtcgtgac ggtcgatccg ccgcgcatac tgctgctcgt
     6601 ggccgtcgtc tacgcgctct caggcccggg gcaactcgcc gtgcagcgct tgcgcatgcg
     6661 ccggaacgct ccttgaaaag cgcattttcc aggacgtttt ggcgattttc tgcagcgcaa
     6721 tgcattcctg atgcgcgctg tggcggatga tcttcatcgg gctctcggtg gccctgctgt
     6781 caagctgaaa cgcccgattc ccagccgtga aaaatacagg cgcacttttt tgccgcgatg
     6841 cttgccacgt ctgaatcgat ccgtataatg cgccttccct gacgggctga ggccaagaag
     6901 ggggggcggc accgaagcgt agccgcagtt gtttaagaag aagagatcaa gcaa
//
LOCUS       DAORUQ010000163         6910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12576_length_6910_cov_5.011962, whole genome shotgun sequence.
ACCESSION   DAORUQ010000163 DAORUQ010000000
VERSION     DAORUQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12576_length_6910_cov_5.011962"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..785
                     /locus_tag="PLW13_12650"
     CDS             204..785
                     /locus_tag="PLW13_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY71274.1"
                     /translation="MHIRNSFRSLLEWQSWDSARIIHSRCGWLFIRTRQRQPEDRMSV
                     RGVNRIIVAVRDIEAAKRYYHELLGAEFRDANWTGAPFGIAVAIAWNVGIELCAPLPG
                     REHDSAVSGFLTQHGEGVMNVYFDVADAEAALARATACGVACQHALDYSQAEIDAHLG
                     GLFRRYQEFVLGTTAQCGFGISLARIEPQGSTG"
     gene            836..1777
                     /locus_tag="PLW13_12655"
     CDS             836..1777
                     /locus_tag="PLW13_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY71275.1"
                     /translation="MATESSRGEWRDASQWQRFLGRLYPGVSIATDPAAGPFRARYRN
                     WSLDHIEFAEIRAISAQRLAVTQPSPQAPESWYLPLQLRGDFRVGQCDQELRAAAGQL
                     VILDSEMPHWRSLEPGSWLLNVRIPKALLGRRHPVLRTRLASPLCAEDGQARIVRDFV
                     LSVWRQRHAITDGQRTALVDALLSLTGVLVFGGAEGVGATPRRSELRRLRLLDCIESH
                     LADPLLDVAFAAAACGMSTRYVHRLMSASGSSFSRHVRDARLQRACRALESDPDAHRT
                     ITRIALDCGFTDPAHFSSTFRRRYGQTPSAWRRRHSP"
     gene            complement(1851..>2300)
                     /locus_tag="PLW13_12660"
     CDS             complement(1851..>2300)
                     /locus_tag="PLW13_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HQY71276.1"
                     /translation="FASMVDPANGISVTVLAQYFTVLLTLVFLASNGHLAMLEVLLES
                     FRYLPAGSGAFGPEPSWTLVSAASWLFLSGLLLALPTVIALLVVNLGFGVMTRAAPQL
                     NVFSLGFPVSMVFGLFIVWASMREFMPRFDMLSAQAFALMRQLAGVP"
     assembly_gap    2301..2400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2479..2748)
                     /locus_tag="PLW13_12665"
     CDS             complement(2479..2748)
                     /locus_tag="PLW13_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HQY71277.1"
                     /translation="MSPDMMVQLLGEGFLLIVLMVSVLIVPSLVVGLVVSVFQAATQI
                     NEQTLSFLPRLLTTLVTVMAAGPWLLAQLIEFTRHVTDTFALVLA"
     gene            complement(2763..3461)
                     /gene="fliP"
                     /locus_tag="PLW13_12670"
     CDS             complement(2763..3461)
                     /gene="fliP"
                     /locus_tag="PLW13_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582812.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HQY71278.1"
                     /translation="MLDAGGALGVPAMKVTTNPDGSQDYTVTIQILALMTALTLLPAL
                     LMMVTAFTRIIVVFAILRQALGLQQTPSNQILLGLALFLTLFVMQPVLDVADEQALQP
                     YLREEIGAREALERVREPFARFMLAQTRESDLDMFLRISGTGPVAGPDAVPFMVLAPA
                     FVTSELKTAFQIGFLLFVPFLVIDLVVASVLMAMGMMMLSPMIISLPFKIMLFVLVDG
                     WALVIGSLASSFAI"
     gene            complement(3566..3904)
                     /gene="fliO"
                     /locus_tag="PLW13_12675"
     CDS             complement(3566..3904)
                     /gene="fliO"
                     /locus_tag="PLW13_12675"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HQY71279.1"
                     /translation="MGEGIGLAPLAEIAAGLALVVVVIFAIAWVLRRLQVGGVAGGRQ
                     MKVVAGLALGPRERVLVVDVGGTQLLLGVTPTQITRLHEFPDPVIKVQPGSGDFALRL
                     RQALGRGESS"
     gene            complement(3835..4305)
                     /locus_tag="PLW13_12680"
     CDS             complement(3835..4305)
                     /locus_tag="PLW13_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71280.1"
                     /translation="MIDMPELTDARLTLDDAGVATLILARDDVRNALTGTGLIPDIVN
                     TCDWINRNRAVGALVLTGDGRAFSSGGNINGMRDTRGGLAPEWAPRGAHFFGSRKGCA
                     VLARGLLLHRESGRRRKIGATAAWRYRQQPHPWARESAWRPWRKSPRGSRSWWW"
     gene            complement(4320..5348)
                     /locus_tag="PLW13_12685"
     CDS             complement(4320..5348)
                     /locus_tag="PLW13_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY71281.1"
                     /translation="MNEQQRMLADTVGRLFPGIAAEQRGHGARGDFAGAWERVRELGI
                     DEVLVPEEAGGFGGNWQDAAVVMHACGQHALPLPVAETLVARKLLCGTDIAAPPGAAI
                     TLAACAGASVAPGGVTLRLSSVPWGGGEACVLTAFEHEGVEYLALYDTAAASQRRARD
                     CAASEPRFDLVLDESALLASCRCEHARRDLRACGALLRAVQTGGALQGALALTIAHVN
                     ERVQFGRALSKFQVVQHQLALLAEEAAAVSCAAGAACLAADTGDATLAIACAKLRANR
                     SVHQATSIAHQLHGAIGFTREHALHHFTQRLWAWRSDFGNDRFWADSLGRMVTRGGAS
                     NLWSRVTG"
     gene            complement(5345..6493)
                     /locus_tag="PLW13_12690"
     CDS             complement(5345..6493)
                     /locus_tag="PLW13_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71282.1"
                     /translation="MNSFNFEPLALPAQTAGLRREVREFLREELAGMTPRERAHTWQG
                     FDAGFSRKLGEKGWIGMTWPREYGGQERSVLERYVLLEELLAAGAPVAAHWIADRQSG
                     TLLLRFGREEVRRRYLPAMACGELFCCIGMSEPNSGSDLASIRTRATRVDGGWKVNGS
                     KLWTTNADKAHLMIALVRTSREEDSRHAGLSQFIIELSSPGVTVKPVVDHTGAAHFGE
                     VFFSDVFVADEMLLGNEGDGWAQVNAELALERSGPERYLSSYRLLEELVAAAGPDPSP
                     RLTELIGELTAELWTLRQMSLSVAGQLAGGGTPALEAAIVKDLGARFEQELPPLVQAA
                     LDDEALGSSGTALQDVLDYLLRASPAFSLRGGTREILRGIIAKGLGLR"
     gene            complement(6503..>6910)
                     /locus_tag="PLW13_12695"
     CDS             complement(6503..>6910)
                     /locus_tag="PLW13_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71283.1"
                     /translation="ARLAARRAYREAGIADPLEELDLVEVHDCFSITELITMEDLGLA
                     QVGQGWRDVLAGKFDRDGELPCNVDGGLKCFGHPLGATGLRMAYEIYLQIHGRAGERQ
                     LVDPKLGLTHNLGGFPNRNVASVAIVGRHDLDN"
BASE COUNT         1154 a         2382 c         2234 g         1040 t
ORIGIN      
        1 gcaggacgcg gcgcggagct acatggacgt actcgtgcgt gcctcgcagg acaatggcgg
       61 gcagcacagc gggcgacgca ccatgtcact cgccgcaacc tgatggctct tgcgacacca
      121 tctgaaagcg gacccgcagc cgaacgaatg tccgcgcagg ttcagcctgt ggccgaacaa
      181 tggtcttcgc caaaggaagg tctgtgcaca tccgcaacag cttccgttcg ctcctggaat
      241 ggcagtcgtg ggattccgcg cgcatcatcc acagccgctg cgggtggttg ttcatccgca
      301 cacgacaaag acaaccggag gatcggatga gcgtcagggg agtcaaccgg atcatcgtgg
      361 cggtgcgtga catcgaggcc gccaagcgtt attaccacga actgctgggc gccgagtttc
      421 gcgacgcgaa ctggactggc gccccgttcg gcatcgccgt tgccattgca tggaacgtcg
      481 gcatcgaact gtgcgccccc ttgccggggc gcgagcacga cagtgcggtg tcgggctttt
      541 tgacgcagca tggtgagggg gtcatgaacg tctatttcga cgtggccgat gccgaagctg
      601 cgctggcccg ggcaaccgcc tgcggagtgg cctgtcagca tgcgcttgat tacagccagg
      661 cggaaatcga tgcacatctg ggcggactgt tccgccgtta ccaggagttc gtgctcggaa
      721 ccacggcaca gtgcggattc ggcatctctc tggcgcgtat cgagccccaa ggatcgacag
      781 gctgaccgca ttgcggctag ggtgagcgca ttcgaggcgc atgggtatcc ggaacatggc
      841 gacggaatcg agtcgagggg agtggcgcga tgcctcgcaa tggcaacgct tcctgggtcg
      901 gctttatccc ggggtgtcga tcgcgaccga tcccgccgcc gggccgtttc gcgcccgcta
      961 ccgcaactgg tctctggacc acatcgaatt cgccgagata cgcgctatct cggcccagcg
     1021 actggcggtt acgcaaccta gtccgcaggc accggagtcc tggtacctgc cgttgcagtt
     1081 gcgcggggat ttccgcgtgg ggcaatgcga ccaggaatta cgtgcagcgg ccggccagct
     1141 ggtgattctg gacagcgaga tgccgcattg gcgcagcctc gagcccggct cgtggctgct
     1201 caacgtgcgc atacccaagg cgctgctcgg acgacgccat cccgttctgc gcactcgatt
     1261 ggcctctccg ttatgcgcgg aagacgggca ggcgcgtatc gtccgggact tcgtattgtc
     1321 ggtctggcgg cagcgccacg cgatcaccga cggacagcgc accgcactgg tggatgcgtt
     1381 gctcagcctg accggagtgc tggtgttcgg cggcgccgaa ggtgtcggcg cgaccccacg
     1441 gcgcagcgaa ctgcgccggc tgcgtctgtt agactgcatc gagtcccatc tcgcggatcc
     1501 tctgctcgac gtggccttcg cggcggctgc ctgcgggatg tcgacgcgtt atgtgcaccg
     1561 gctgatgagc gcgtcgggaa gcagtttctc gcggcatgtg cgcgatgcgc gcctgcagcg
     1621 tgcctgccgc gcgctggaga gcgatcccga tgcgcaccgg acgatcacca ggatcgcact
     1681 ggattgcggc ttcaccgatc ccgcgcattt cagcagcacg ttccggcgcc gatatggcca
     1741 gacaccgagc gcgtggcggc gccggcactc accttgatcg gtgggattgt tgttccgctc
     1801 tccgaccggg ggagaatgtc cgcgcaccac acatcggggt gcgcgacctc tcacggcaca
     1861 ccggcaagct ggcgcatcag tgcgaaggcc tgcgccgaca gcatgtcgaa gcgcggcatg
     1921 aactcgcgca tgctcgccca gacgatgaac agcccgaaca ccatcgagac cgggaagccg
     1981 agcgagaata cattcagctg cggcgcggcg cgcgtcatca cgccgaagcc aaggttcacg
     2041 accagcagcg cgatcacggt gggcagagcg agcagcaagc cgctcaagaa cagccacgat
     2101 gccgccgaca ccagggtcca gctcggctcg ggaccgaagg cccctgagcc tgccggcagg
     2161 tagcggaagc tctccagcag cacctctagc atcgcgagat gaccgttgct ggcgagaaaa
     2221 accagcgtca gcagcaccgt gaaatattgc gccagcaccg tgacgctgat gccgttggcc
     2281 ggatcgacca tcgaggcgaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 ccgctcaggc gaaacagcgg gaacagccac gcgcccagcc attgcgacag cgcgggcagc
     2461 gcgagatcga tcatggcttc aggcgagcac cagcgcgaag gtgtcggtga cgtgccgcgt
     2521 gaactcgatg agctgcgcca gcagccaggg tccggccgcc atcaccgtga ccagggtggt
     2581 cagcaggcgc ggcaggaagc tcagcgtctg ctcgttgatc tgcgtggcgg cctggaacac
     2641 gctgaccacc aggcccacca cgagactggg cacgatcagc acgctcacca tcagcacgat
     2701 cagcaggaag ccctcgccga gcagctgcac catcatgtcg gggctcatcg tggcacccct
     2761 ccctagattg cgaagctcga ggccagcgag ccgatgacca gcgcccagcc gtccaccagc
     2821 acgaacagca tgatcttgaa cggcagcgag atgatcatcg gcgagagcat catcatgccc
     2881 atcgccatca gcacgctggc gaccacgagg tcgatcacca ggaacgggac gaacagcagg
     2941 aacccgatct ggaacgcggt cttcagctcg ctggtcacga aggccggcgc cagcaccatg
     3001 aacggcacgg cgtcgggccc ggccaccggg ccggtaccgg agatgcgcag gaacatgtcg
     3061 agatcgctct cgcgcgtctg tgccagcatg aacctggcga aaggttcgcg cacgcgctcc
     3121 agcgcctcgc gcgcgccgat ctcctcgcgc aggtagggct gcaacgcctg ttcgtcggcc
     3181 acatccagca ccggctgcat cacgaacagc gtgaggaaca gcgccagccc cagcaggatc
     3241 tggttcgagg gcgtctgctg caagccgagc gcctggcgca ggatcgcgaa caccacgatg
     3301 atgcgcgtga aggcggtcac catcatcagc agcgccggca gcagcgtcag cgccgtcatc
     3361 agcgccagga tctggatcgt gaccgtgtag tcctggctgc cgtcgggatt ggtggtgacc
     3421 ttcatcgccg gcacgccgag cgcgccgccg gcatcgagaa tcgaggcagc gccggtctgc
     3481 gccagcgcga gtccgggcag caacagcagc gcggccagca gccacgctgc ggcgccgagc
     3541 ggccgcgccg aacccgcgcg gagcctcacg atgactcgcc ccggccgagc gcctgtcgca
     3601 agcgcaacgc gaagtcgccg ctgcccggct ggaccttgat caccgggtcg gggaattcgt
     3661 gcaggcgcgt gatctgcgtc ggcgtgaccc cgagcagcag ctgcgtgccg ccgacatcga
     3721 ctaccagcac ccgctcacgc ggtccgagcg caagcccggc caccaccttc atctgcctgc
     3781 ccccggccac accaccgacc tgcagccggc gcagcaccca ggcgatcgcg aagatcacca
     3841 ccaccacgag cgcgagcccc gcggcgattt ccgccagggg cgccaggccg attccctcgc
     3901 ccacgggtgt ggttgctgcc gataccgcca tgctgctgtc gcgccaattt tccgtcgtcg
     3961 tcccgattca cggtgcaaca gcaagccgcg tgccagcacg gcgcaacctt ttcgcgaacc
     4021 aaagaaatgg gccccgcgag gggcccattc gggagcaagc ccccccctgg tgtcgcgcat
     4081 cccgttgatg ttgccgcccg aggaaaaggc cctgccatcc ccggtgagca ccagtgcgcc
     4141 gaccgcgcgg ttgcggttga tccagtcgca ggtattcacg atgtcgggaa tcagaccggt
     4201 gccggtcagg gcgttgcgga catcgtcgcg cgccaggatc agcgtggcga caccggcgtc
     4261 gtccagcgtg agacgcgcgt cggtgagctc cggcatgtcg atcatcgtta cactcccttt
     4321 cagccggtga cccggctcca caggttcgag gcgccaccgc gcgtcaccat ccgtcccagg
     4381 ctgtccgccc agaaacggtc gttgccgaaa tcggagcgcc acgcccacag gcgctgcgtg
     4441 aaatggtgca gcgcgtgctc gcgcgtgaag ccgatcgcgc cgtgcaactg gtgcgcgatc
     4501 gaggtggcct ggtgcacgga gcggttggca cgcagcttcg cgcaggcgat cgccagggtg
     4561 gcatcgcccg tatcggcggc aaggcaggca gctcccgccg cgcagctcac cgcggcggcc
     4621 tcctcggcca gcagcgccag ctggtgctgc acgacctgga acttgctcag tgcgcgcccg
     4681 aactgcacgc gctcgttgac gtgcgcgatg gtcagcgcga gcgctccctg cagtgcgcca
     4741 cccgtctgca ccgcgcgcag caatgcgccg caggcgcgca ggtcgcgccg tgcgtgctcg
     4801 cagcggcagg acgccagcag cgcgctctcg tcgagcacca gatcgaagcg cggttcgctg
     4861 gcggcgcaat cgcgcgcgcg ccgctgcgac gcggccgcgg tgtcgtacag cgcgaggtat
     4921 tccacgccct cgtgctcgaa agcggtcagt acgcaggcct cgcccccgcc ccagggcaca
     4981 ctggagaggc gcagcgtcac gccccccggt gcaacgcttg ctccggcgca cgcggcaagg
     5041 gtgatcgcgg ccccgggcgg cgcggcaatg tcggtgccgc agagcagctt gcgcgccacc
     5101 agcgtctctg cgaccggcag cggcagcgcg tgctggccgc aggcatgcat cacgaccgcc
     5161 gcatcctgcc agttcccgcc gaagccgccc gcctcctccg gtaccagcac ctcgtcgatg
     5221 ccgagctcgc gcacgcgctc ccacgcgccg gcgaagtccc cgcgcgcgcc gtgcccgcgc
     5281 tgctctgccg cgatccccgg aaacaggcgc ccgaccgtat cggcgagcat gcgttgctgt
     5341 tcgttcatct gagtccgagc cccttggcga tgatgccgcg caggatttcg cgggtgccgc
     5401 cgcgcagcga gaaagccggc gatgcccgaa gcaggtagtc cagcacatcc tgcagcgccg
     5461 tgcccgagga cccgagggcc tcgtcgtcca gcgcggcctg caccagcggc ggcagctcct
     5521 gttcgaagcg cgcaccgaga tccttcacga tggccgcctc gagcgcgggt gtgccgcccc
     5581 cggcgagctg tcccgcgacc gatagcgaca tctggcgcag cgtccacagc tccgccgtga
     5641 gctcgcctat cagttccgtg agccggggcg aggggtcggg tccggcggcc gccaccagtt
     5701 cctcgagcag acggtagctg ctgagataac gctccggccc gctgcgctcg agcgccagtt
     5761 ccgcattgac ctgcgcccag ccgtctccct cgttgccgag cagcatctcg tcggcgacga
     5821 agacgtcgct gaagaatacc tcgccgaaat gcgccgcgcc ggtgtggtcg accaccggtt
     5881 tcaccgtgac gcccggactg gacagctcga tgatgaactg gctgagcccg gcgtggcggc
     5941 tgtcttcttc cctgctggtg cgcaccagcg cgatcatcag gtgcgccttg tcggcgttgg
     6001 tggtccacag cttggaaccg ttgaccttcc agcccccgtc gacgcgcgtg gcgcgggtcc
     6061 tgatcgaggc caggtcggac cccgaatttg gctcgctcat gccgatgcag cagaacagct
     6121 cgccgcacgc catcgccggc aggtagcgcc ggcgcacctc ctcgcggccg aagcgcaaca
     6181 gcagcgtgcc gctctggcgg tcggcgatcc agtgcgcggc gaccggcgca cccgcggcca
     6241 gcagttcctc gagcaatacg tagcgctcca gcacgctgcg ctcctggccg ccgtactcgc
     6301 gcggccaggt catcccgatc catcccttct cgccgagctt gcggctgaac ccggcatcga
     6361 agccctgcca ggtgtgcgcg cgctcgcgcg gcgtcatgcc cgcgagttcc tcgcgcagga
     6421 attcgcgcac ttcccggcgc agccccgcgg tttgcgccgg aagcgccagc ggttcgaaat
     6481 tgaaactgtt cacgcccgct cctcagttgt ccaggtcatg gcgcccgacg attgcgacgc
     6541 tggcgacatt acgattggga aagccgccaa gattatgcgt caggcccagt ttcgggtcga
     6601 ccagctggcg ctcgccggcg cgcccgtgga tctgcaggta gatctcgtag gccatgcgca
     6661 accccgtggc gcccagcgga tgaccgaagc atttcaggcc gccatcgacg ttgcacggca
     6721 gctcgccgtc gcggtcgaac ttgccggcca gcacgtcccg ccagccctgc cccacttgcg
     6781 ccagtcccag gtcctccatc gtgatcagct cggtgatcga gaagcaatcg tgcacctcga
     6841 ccaggtcgag ttcctcgagc gggtccgcga tgcccgcctc gcggtaggcg cggcgcgcgg
     6901 ccaggcgcgc
//
LOCUS       DAORUQ010000164         6834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12790_length_6834_cov_6.286325, whole genome shotgun sequence.
ACCESSION   DAORUQ010000164 DAORUQ010000000
VERSION     DAORUQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12790_length_6834_cov_6.286325"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLW13_12700"
     CDS             complement(<1..688)
                     /locus_tag="PLW13_12700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQY71284.1"
                     /translation="MPDLDHPDNPFASEEITPAYSAEWDARRRVAAAIRRLTEVLVSS
                     TPPIPQLHAIADTLELTADSFAAQPRIYGRALFAGGGAHGSMGQISHELNPVAGISNP
                     LAPPLNVWIADGVTHGRATLGWAYEGPPGSVHGGFVAALFDQFMGVAQASGGQPGMTG
                     TLSTRYHRRTPLNTELHFRGWVEKIEGRKTFVRAEIHAGEVLTASCDGVFVQPLGGMD
                     RLHREYAQSPS"
     gene            complement(739..1692)
                     /gene="glk"
                     /locus_tag="PLW13_12705"
     CDS             complement(739..1692)
                     /gene="glk"
                     /locus_tag="PLW13_12705"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HQY71285.1"
                     /translation="MPPRPQRLVADIGGTNTRFALADARGRLHAPLTLATRRFDSLAD
                     AARSYLESTGARPLEGCIAVAGPVSGDAVRLTNHRWEFSIAALREGLGLQRLRVINDF
                     EAIALALPALAADELLQIGGGEPVRGSPMAVLGPGTGLGVAQLIATRGGFQPVSTEGG
                     HASIGPCDEAELQILATLMRNGQHLCREALLSGPGIERIYWALCLAAGSAYHGYSAAE
                     IQGHAAAGSDATCRAALEHFCAFLGTAAGDQALCCGAQGGVFIAGGIVPRFTEFLRHS
                     RFRERFEAKGPMRDYVSAIPVFVITRENPGMLGAARADLHA"
     gene            1919..2740
                     /locus_tag="PLW13_12710"
     CDS             1919..2740
                     /locus_tag="PLW13_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY71286.1"
                     /translation="MRFSRLDALLLVAVIAAIVLALSGVLEPGPRLPRDAVAAVNGRA
                     IGAGAFAAQLDAVAVQLERAPDVSERAALLERVIDEELLLQQGLALELPRKDARLRAQ
                     LVQEVIRQALAQSARAPVTEDELRAFHGAHSGFFRQPATYRVWRFDCRDATAARALQA
                     GLGAGADPDPRGGCRRNALLPDAWLGAAKLGDLLGAELASAAVALAPGETLLSERADG
                     ASVLLLLALAPAREPAFEQVRAQVETEFRRRADEAALAAYLERLRAGAELRIGGG"
     gene            2737..3912
                     /locus_tag="PLW13_12715"
     CDS             2737..3912
                     /locus_tag="PLW13_12715"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQY71287.1"
                     /translation="MTVRALLLLALALAGGAARADWQARSWSRWQFEGSVAEAVFGIE
                     RTELARLRGSTDAAWFTAELRASVALQEGGQACALRDAVALGTGDTVRVQLRWRCPGE
                     IRAPQVRVGLLLDVERDPLHHASFTHADGRHEEVLFTRDAPQRALAAAPRPLPAAATV
                     LRYLGLGFSHILAGLDHVAFLLCMLLVAARLSSRLWMITGFTLGHSITLSLSVLGLVS
                     VRSAAVEALIGYTVALLAAEACLRHGLRSAPWQLWLVVAGIGAITWAGSGALAPPTVV
                     ALLLLTPAYLVLAQRCHGNDALHLAMTAVFGLVHGLGFAGVLNAIGVPDGQRGWALAG
                     FNIGVELGQIALLAAFALLLQAPHLLRSPALARGATRASAIALCALGVYWLAGRSVG"
     gene            4052..5155
                     /gene="gcvT"
                     /locus_tag="PLW13_12720"
     CDS             4052..5155
                     /gene="gcvT"
                     /locus_tag="PLW13_12720"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQY71288.1"
                     /translation="MKQTPLHALHLELGARMVPFAGYEMPVQYPAGIMREHLHTRESA
                     GLFDVSHMGQVLVRGAGATAALERLVPADLVALAPGRQTYSLFTNAEGGVLDDLIITR
                     WSDDTFFVVVNAGCKEQDLAHLRAGLPGIAVEALAGQALLALQGPRAAAVMTRFAPAL
                     ASLVFMSGTHCEIGGVACFVTRSGYTGEDGFEISVPADAAEALARRLLACPEVMPIGL
                     GARDSLRLEAGLCLYGHDLNPQVTPVEAGLQWAIAPARRAGGARAGGYPGAARIAAQM
                     QSGPARRRIGLLGDGKAPVREGSVLTDLAGRAVGEVCSGGFGPTVGGPVLMAYAESAC
                     AAPGTQLLALVRDKPRPVTVTKMPFVPQRYHRG"
     gene            complement(5143..5667)
                     /locus_tag="PLW13_12725"
     CDS             complement(5143..5667)
                     /locus_tag="PLW13_12725"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQY71289.1"
                     /translation="MTEQRRGVTLIGMPGAGKSTVGVLLSKRLALDFVDSDLAIQVRE
                     GRTLQQIIDAGGHLRLREIEAAVLLGIDPHGRVVATGGSAIYSDAAMRHLGAGSTIVY
                     LDVPLAELRRRITDYDTRGIARRPEQGFEELFAERTRLYRRYAAIRIDCADLGQEQLL
                     ERISAALLSGSQPR"
     gene            5748..>6834
                     /locus_tag="PLW13_12730"
     CDS             5748..>6834
                     /locus_tag="PLW13_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQY71290.1"
                     /translation="MRAEGTARGADAAWEALAGHFAQSRGLDLREAFVTDPGRAQRLR
                     VSAAGLTLDYSKNLVNARTIELLCALARAREVSGRARAQLGGAMVNGTEERAVLHTAL
                     RGTDAGERLAQAREVQAVHRRMAELVRRVRAGDWAGWSGEPVRDVVNIGIGGSHLGPV
                     FATEALGHLHGDGTRCHFVSNVDPRDLEVVLAGLAPSRTLFIVASKTFSTLETLENAR
                     SARRWLQAAGCPQTALDRHFLAVSSNVPRAVDFGIAADNVLPMWDWVGGRYSLWSAIG
                     LPIAMAVGMEAFDELLAGAHAMDLHFRDAPPESNMTILLALIGVWYRNFHGTSSHAVI
                     PYDQSLARLPEFLQQLVMESNGKSVARD"
BASE COUNT          859 a         2327 c         2581 g         1067 t
ORIGIN      
        1 ccgacggcga ctgcgcgtac tcgcgatgca gccggtccat gccgcccagg ggctgcacga
       61 aaacgccgtc gcagctcgcc gtgagcactt cgccggcgtg gatctcggca cgcacgaagg
      121 tcttgcggcc ctcgatcttc tcgacccagc cgcgaaagtg cagctcggtg ttgagcggtg
      181 tgcgccggtg atagcgcgta ctgagcgtgc cggtcatccc cggctgcccg ccgctcgcct
      241 gcgccacgcc catgaactgg tcgaacaggg ccgcgacgaa cccaccgtgc acgctgccgg
      301 gcggtccttc ataggcccag cccaaggttg cgcgcccgtg ggtcacgccg tcggcaatcc
      361 acacgttgag cggcggtgcc agcggattgc tgattccggc gacgggattc agttcatggc
      421 tgatctgtcc catgctgccg tgagcgcccc cgccggcgaa gagggcacgg ccgtagatcc
      481 tcggctgtgc ggcaaagctg tcggcggtca gttccagtgt gtcggcgatg gcatggagct
      541 gcgggatcgg cggggtgctg ctgaccagca cctcggtgag ccggcggatc gccgcggcga
      601 cgcggcggcg cgcgtcccac tccgcgctgt aggcgggtgt gatctcctcc gaggcgaagg
      661 ggttgtcggg atggtcgagg tccggcaagt ctttcactcc tgcatcgatg cgtgtgacag
      721 ggcgctgatt gtagcgcctc aggcgtggag atcggcgcgc gccgcgccga gcatgccggg
      781 attctcgcgc gtgatcacga acaccgggat cgcgctcacg tagtcgcgca tcggcccctt
      841 ggcctcgaag cgctcgcgaa agcggctgtg acggaggaat tccgtgaagc gcggcacgat
      901 accgccggcg atgaagacgc caccctgcgc gccgcagcac agcgcctggt cgccggcggc
      961 ggtgccgagg aaggcgcaga aatgttccag cgccgcgcgg caggtcgcgt ccgatcccgc
     1021 cgccgcgtgc ccctgtattt cggctgccga gtagccgtgg taggcgctgc ccgcggccag
     1081 gcacagcgcc cagtagatgc gctcgatgcc gggacccgag agcagcgcct cgcggcagag
     1141 gtgctggccg ttgcgcatca gggtggcgag gatctgcagt tccgcctcgt cgcagggtcc
     1201 gatcgaggcg tggccgcctt cggtggagac cggctggaag ccgccgcgtg tcgcgatcag
     1261 ttgcgccacg ccgagcccgg tgccgggtcc cagcaccgcc atcgggctgc cgcgcaccgg
     1321 ctcgccgccg ccgatctgca gcagttcgtc cgcggccagc gccggcagcg ccagcgcaat
     1381 ggcctcgaaa tcgttgatca cgcgcagccg ctgcagcccc agcccttcgc gcagggccgc
     1441 aatcgagaac tcccagcggt gattggtcaa tcgcaccgcg tcgccgctca ccgggccggc
     1501 gacagcaatg caaccctcga gcgggcgcgc tccggtcgac tcgaggtagc tgcgggccgc
     1561 gtcggccagc gagtcgaagc ggcgcgttgc cagcgtgagc ggcgcgtgca gccggccccg
     1621 cgcgtcggcc agcgcgaagc gggtgttggt gccgccgata tcggcgacca gtcgctgggg
     1681 acgaggtggc atctgggtcg gtgcgcgtgt gccgggatgg ccggattctg gatgcatgcc
     1741 ccgccgagat caatgcatac ttgcccgggc gccgcgcaac gtccagcaac cacggccgcg
     1801 atggagttcc tcatgagcga tcccgcatca ccgcccggac gcgacgcagc cggcgccacc
     1861 gcgcgttgca tgcgccggca cgttgcgccc tgacgcacgg cctgagcgcg tggtcccgat
     1921 gcgattttcg cgcctcgatg cgctgctgct ggtcgcggtg atcgccgcca tcgtgctggc
     1981 gctgtccggt gtcctcgagc ccggaccgcg actgccccgg gacgcggtgg ccgcggtgaa
     2041 cggcagggcg ataggtgccg gggctttcgc ggcgcaactc gatgccgttg ccgtgcagct
     2101 cgagcgcgcc cccgacgtgt ccgagcgcgc cgcgctgctc gagcgcgtga tcgacgagga
     2161 actgttgttg cagcaggggc ttgcgctcga gttgccgcgc aaggacgcgc ggctgcgcgc
     2221 gcagctggtg caggaggtca tccgccaggc gctggcgcag tcggcacgcg cacccgtcac
     2281 cgaggacgag ttgcgcgcat tccatggcgc tcattcgggc ttcttccgtc agcctgccac
     2341 ctaccgcgtc tggcgtttcg attgccgtga cgccaccgcg gcgcgtgcgc tgcaggccgg
     2401 gctcggcgcg ggtgctgatc cggacccgcg gggcggctgc cggcgcaacg cgctgctgcc
     2461 cgatgcctgg ctgggcgctg cgaagctcgg cgatctgctc ggcgcggagc tcgcgtccgc
     2521 cgcggtcgcg ctcgcccccg gcgagaccct gctgagcgag cgcgccgatg gcgcgagcgt
     2581 gctgttgctg ctcgcgctgg ctcctgcgcg cgaaccggca ttcgagcagg tgcgcgcgca
     2641 ggtggagacg gaattccgcc gccgcgccga cgaggcggcg ctggccgcgt acctcgagcg
     2701 cctgcgtgcg ggcgccgaac tgcggatcgg cggcgggtga ccgtgcgcgc cttgctgctg
     2761 ctcgcgctcg cgctcgccgg cggggctgcg cgggccgact ggcaggcgcg ctcctggtcg
     2821 cgctggcagt tcgaaggctc ggtcgccgag gcggttttcg gcatcgagcg aacggagctg
     2881 gcgcggctgc gcggcagcac cgacgcggcc tggttcaccg cggagctgcg ggcgagcgtg
     2941 gcgttgcagg agggcgggca ggcgtgcgcg ctgcgcgatg ccgtggcgct gggaaccggc
     3001 gacacggtgc gggtccagtt gcgctggcgc tgtcccgggg aaatccgcgc gccgcaggtc
     3061 cgggtgggac tgttgctgga cgtggaacgc gatcccctgc atcacgccag cttcacccat
     3121 gccgacggcc gccacgagga ggtgctgttc acgcgcgacg cgccgcagcg cgcgttggcg
     3181 gcggcgccgc gcccgctgcc ggccgcggct accgtgctgc gttatctcgg gcttggtttc
     3241 tcgcacatcc tggccgggct cgatcacgtc gctttcctgc tgtgcatgct gctggtggcc
     3301 gcgcggctct ccagccgcct gtggatgatc acgggcttca cgctcggaca cagcatcacg
     3361 ctttcgctgt cggtcctggg cctggtgagc gtgcgcagtg ccgcggtcga ggcgctgatc
     3421 ggctacacgg tggcgctgct cgcggccgag gcctgcctgc gccacgggct gcgctcggcg
     3481 ccgtggcagc tgtggctcgt ggtggcaggt atcggtgcaa tcacctgggc cgggagtggc
     3541 gcgctcgcgc cgccaaccgt ggtcgcgctg ctgctgctca cgccggccta cctcgtactg
     3601 gcgcagcgct gtcacggcaa tgacgcgctg catctggcga tgacggcggt gttcgggctg
     3661 gtgcacgggc tcggctttgc cggcgtgctg aacgcgatcg gcgtgccgga tgggcagcgc
     3721 ggctgggcgc tcgcgggctt caacatcggt gtggagctgg ggcagatcgc gctgctcgcg
     3781 gcgttcgcgc tgctgttgca ggcaccgcac ctgctgcggt cgccggcgct ggcgcgcggc
     3841 gccacccggg cgagcgcgat cgcgctgtgc gcgctcggcg tgtactggct cgccgggcgc
     3901 agcgtcggct gagagggccg gaccacgctg cgccgggtct tgcgtgaccc gcgcgcttta
     3961 cgcccgcgca tcgaatgcgc agaatgcccg ccttcgcggt gcctggaccc gggcgccgtg
     4021 cctcaggcct gacatcccac ggggggtcgc gatgaaacag acgccgctgc atgcgctgca
     4081 cctggaactc ggtgcgcgca tggtgccttt cgccggttac gagatgccgg tgcagtaccc
     4141 ggcggggatc atgcgcgaac acctgcacac ccgcgagagc gcgggcctgt tcgatgtctc
     4201 gcacatgggc caggtgctgg tgcgcggcgc cggggcgacg gccgcgctgg agcgcctggt
     4261 gcccgcggac ctcgtggcgc tggcgccggg acgccagacc tactcgctgt tcaccaatgc
     4321 cgagggcggc gtgctcgacg acctgatcat cacgcgctgg tcggatgaca ccttcttcgt
     4381 ggtcgtgaac gcaggctgca aggagcagga cctcgcgcac ctgcgcgccg gactgccggg
     4441 catcgccgtc gaggcgctgg ccgggcaggc gctgctcgcg ctgcagggcc cgcgcgcggc
     4501 cgcggtgatg acgcgcttcg cgccggccct ggcctcgctg gtgttcatga gcggcacgca
     4561 ctgcgagatc gggggcgtcg cctgcttcgt gacccgctcg ggctatacgg gagaggacgg
     4621 cttcgagatc tcggtgcccg cggacgccgc cgaggccctg gcgcggcgcc tgctcgcgtg
     4681 tcccgaagtg atgccgatcg gcctgggggc gcgcgattcg ctgcgcctcg aggcgggtct
     4741 ttgtctgtac gggcacgacc tgaatccgca ggtcacgccg gtcgaggcgg ggctgcagtg
     4801 ggccatcgca ccggcgcgac gtgccggtgg ggcgcgcgcc ggcggctatc cgggtgcggc
     4861 gcgcatcgcg gcgcagatgc agtccggacc ggcgcgccgg cgcatcgggc tgctcggcga
     4921 cggcaaggcg ccggtgcgcg agggctcggt gctgacggat ctcgcggggc gcgcggtcgg
     4981 cgaggtgtgc agcggcggct tcggtcccac cgtgggcggg ccggtcctga tggcctatgc
     5041 cgagagcgcg tgcgcggcgc cgggtacgca gctgctggcg ctggtgcgcg acaagccgcg
     5101 cccggtgacc gtcacgaaga tgccgttcgt gccgcagcgc tatcaccgcg gctgagagcc
     5161 cgagagcagc gcggcgctga tgcgctcgag cagctgctcc tggccaaggt cggcgcagtc
     5221 gatgcggatc gcggcgtagc gccggtagag tcgcgtgcgc tcggcgaaca gttcctcgaa
     5281 gccctgttcc gggcgcctgg cgatgccgcg ggtgtcgtag tcggtgatgc gccggcgcag
     5341 ctcggcgagc ggcacgtcga ggtaaacgat ggtgctgccg gcgccgaggt ggcgcatggc
     5401 ggcgtcgctg tagatcgcgc tgccgccggt ggcgaccacg cgcccgtgcg gatcgatgcc
     5461 gagcagcacg gcagcctcga tctcgcgcag ccgcaggtgg cccccggcgt cgatgatctg
     5521 ctgcagcgtg cgcccctcgc gcacctggat cgccagatcg gaatccacga agtccagcgc
     5581 caggcgcttc gagagcagca ctcccaccgt gctcttgccg gccccgggca tgccgatcag
     5641 cgtgactccg cgtcgttgtt ccgtcattgc gtggtgctat acagtgcaag ggggctcgcg
     5701 cattctgcga gccggtatga cggaccgcaa tagccgagga ggaacgggtg agggcagagg
     5761 gaacggcgcg tggcgccgac gcggcgtggg aagcgttggc cgggcatttc gcgcagtcgc
     5821 gcggcctgga tctgcgcgag gcctttgtca cggatccggg acgcgcgcag cgcctgcgag
     5881 tgagcgccgc gggactgacg ctcgactact cgaagaatct ggtcaatgcg cgcacgatcg
     5941 agctgctttg cgcgctggcg cgcgcccgcg aggtgtccgg tcgcgcgcgc gcgcagctcg
     6001 gcggcgcgat ggtgaacggc accgaggagc gcgcggtgct gcacaccgcg ctgcgcggca
     6061 ccgatgccgg ggagcggctg gcgcaggcgc gcgaggtgca ggccgtgcac aggcgcatgg
     6121 cggagctggt gcggcgcgtg cgcgccggcg actgggccgg atggagcggc gagccggtgc
     6181 gcgatgtcgt gaatatcggc atcggtggct cgcacctggg gccggtcttc gcgaccgagg
     6241 ccctgggtca cctgcacggc gacggcacgc gctgccattt cgtgtccaat gtcgatccgc
     6301 gcgatctcga ggttgtgctc gccgggctcg cgccgtcacg cacgctgttc atcgtggcct
     6361 ccaagacctt cagcaccctg gagacgctcg agaacgcgcg ctcggcgcgg cgctggctgc
     6421 aggccgcggg atgtccgcag acggcgctgg accgccactt cctcgcggtc tcgtcgaacg
     6481 tgccgcgcgc ggtcgacttc ggtatcgccg ccgacaacgt gctgccgatg tgggactggg
     6541 tcggcggacg ctactcgctg tggtcggcga tcgggctgcc gatcgcgatg gccgtgggca
     6601 tggaggcctt cgacgagctg ctcgccggcg cgcacgcgat ggacctgcat ttccgcgatg
     6661 ccccgcccga gtcgaacatg acgatattgc tggcgctgat cggcgtgtgg taccgaaatt
     6721 ttcacggcac ctcctcgcac gcggtgattc cctacgacca gtcgctcgcg cggttgcccg
     6781 agttcctgca acagctggtg atggagagca acggcaaatc agtcgcgcgt gacg
//
LOCUS       DAORUQ010000165         6824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_12820_length_6824_cov_6.446152, whole genome shotgun sequence.
ACCESSION   DAORUQ010000165 DAORUQ010000000
VERSION     DAORUQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12820_length_6824_cov_6.446152"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="dapE"
                     /locus_tag="PLW13_12735"
     CDS             complement(<1..1146)
                     /gene="dapE"
                     /locus_tag="PLW13_12735"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HQY71291.1"
                     /translation="MSMTQPAWPSATLALAAELIARPSVTPDDAGCQQLLAARLEAMG
                     FACTHLRYGEVDNLWAVRGGEGPLFVFAGHTDVVPPGPLAAWTHPPFEPRIENGMLHG
                     RGAADMKGSLAAMVTACERLLATGAVQGRIAFLLTSDEEGPSIDGTRRVVEWLRERGE
                     QIRWCVVGEPSSTHRVGDVIKVGRRGSLGAELTVRGVQGHVAYPERARNPIHEALAAL
                     AELVATRWDEGNAHFPATSLQISNIHAGQGATNVIPGELYVVFNLRFSTESDAATLKS
                     RITALLARHGIDHDIAWQLHGEPFLTARGELLDSAVAGIAELGAPTPELSTTGGTSDG
                     RFIAPLGCQVIELGPCNATIHKVDECVRAADLDTLSAMYEAIMRRLLC"
     gene            complement(1143..2180)
                     /gene="dapD"
                     /locus_tag="PLW13_12740"
     CDS             complement(1143..2180)
                     /gene="dapD"
                     /locus_tag="PLW13_12740"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQY71292.1"
                     /translation="MTAPLFAIGLGTGTRGARGNWLEVFYPAPLRNVDAALAAALATH
                     CGYAGGNAALEPSAQQLAALATALGAAGHEAQAAIAGQLAGGRGAPVITVLATDAAPA
                     GVPEAYLKLHLLSHRLCRPHEQKLDGLFAVLPNVAWTSEGAIEVAELPARQLAARVAG
                     RTLTVHGVDKFPRMSDYVVPAGVRIADAARVRLGAWLGEGTTIMHEGFVNFNAGTEGP
                     GMIEGRISAGVFVGAGSDLGGGCSTMGTLSGGNKALIAVGRECLIGANAGIGISLGDR
                     CTIEAGLYVTAGSRVTVIDEAGAVVKVVAARELSGGSDMLFRRNSQSGAIECLSNRAA
                     IALNEALHAHN"
     gene            complement(2208..2558)
                     /locus_tag="PLW13_12745"
     CDS             complement(2208..2558)
                     /locus_tag="PLW13_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HQY71293.1"
                     /translation="MLNVYGIANCDSVSAARRWLKEHGIAHEFHDLRKEHVDAQTLEQ
                     WLAALGRERLVNKSSATWRTLERSQREQIESGNPVPVLIANPTLIKRPVLAGDGLLHC
                     GFNPTDYELLFSRS"
     gene            2715..3365
                     /locus_tag="PLW13_12750"
     CDS             2715..3365
                     /locus_tag="PLW13_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQY71294.1"
                     /translation="MSMLPKVEIFAGLSEEEQAALENSCVTRNYPRNTVVINENDFAD
                     SLYVIESGRVKVYCSDKNGKEYIMNTLGPGDYFGELALLDDDRRSASVRTLEKSTFTI
                     MFKDEFHAIMERHPNIATTLIRNLTRRVRKLTENVKSLALQDVYGRVTKVLMTLAEPR
                     GDKLYIEERLTQQDIADRVGASREMVARILKDLAIGDYIAFEGKNIIINGKLPTSY"
     gene            3509..3652
                     /locus_tag="PLW13_12755"
     CDS             3509..3652
                     /locus_tag="PLW13_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71295.1"
                     /translation="MSGRRPQAAWWQELYRSERFNGAERLLLGVAALALTGALVVLVR
                     VVS"
     gene            complement(3662..4567)
                     /locus_tag="PLW13_12760"
     CDS             complement(3662..4567)
                     /locus_tag="PLW13_12760"
                     /inference="COORDINATES: protein motif:HMM:NF001464.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HQY71296.1"
                     /translation="MWFKNLRIYRLTDLLPCPVDELGERLLATSFVPCGGLDTQRSGW
                     VSPMGPTATHLVHSAANFHLVCARTQQRLLPAAAIREVLDEKILEIEAAEGRELRKRE
                     RANLKDEVIQTLLPRALTRSHLTYAFLAESRGLLLIDSSSPARAEDLLNLLRDSLGRL
                     PVKPLVPRHSPVDLMTRWLHGGRLPPGYRLGQQCDMRDPLNAANVVRCRQQELATQEV
                     RQHLDAGKQVSALGLEWNERIGFVLAEDLAVKAIKYSEIVQKDAGAEDEMEAAARFDA
                     DFALMSMELDQLCESLIEVFGIEED"
     gene            4696..5331
                     /locus_tag="PLW13_12765"
     CDS             4696..5331
                     /locus_tag="PLW13_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY71297.1"
                     /translation="MLELIKSGGWVMLPIILGSIAAVAISIERWWTLDYRKVVPPNTL
                     AEVWQLIRRNELSPERLRALKASSPLGRILAAGLANAHAGRDVMKESIEEAASHVIHD
                     LERYLNSLGTIAAIEPLLGLLGTVLGMIQVFSDVMLHGTGNAELLAGGISKALITTAA
                     GLIVAIPALMLHRHFLRRVDTIVVGLEQEAIKLVDAMHGGRPVDVREKPGT"
     gene            5328..5750
                     /locus_tag="PLW13_12770"
     CDS             5328..5750
                     /locus_tag="PLW13_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY71298.1"
                     /translation="MKFRRRQRVEVGLDITPLIDVVFLMLIFFMVSTTFTREAHLQIS
                     LPEADAAPALDEKDRLELIVAASGSYALNGRVLVNSRIETLLAALGEASGGDVTQPLV
                     ITADAKTPHELVVRAMDAAGRLGFSRLSITTRLPQEGG"
     gene            5800..6564
                     /gene="kdsB"
                     /locus_tag="PLW13_12775"
     CDS             5800..6564
                     /gene="kdsB"
                     /locus_tag="PLW13_12775"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQY71299.1"
                     /translation="MTFSVIIPARFASTRLPGKPLLDIAGQPMIRHVWERACRSSAAR
                     VVIATDDERILEAARAFGAEVCLTGAHHQSGTDRLQETVATLGLGAGEIVVNVQGDEP
                     LIPPAVIDQVAANLAANRAHGIATLCEEITAIGHFLDPNVVKVVCAEDGSALYFSRAP
                     IPWPRDAFARSREEMPAGLRAYRHIGIYAYRVDFLNRFVGWPVGTLEGFESLEQLRAL
                     EHGVGIHVAQACAPISGGVDTPADLARVDALLRGDG"
BASE COUNT         1050 a         2314 c         2384 g         1076 t
ORIGIN      
        1 gcagagcaac cggcgcatga tcgcctcgta catcgcgctc agcgtgtcga gatcggcggc
       61 gcgcacgcat tcgtcgacct tgtggatggt ggcgttgcag gggccgagct cgattacctg
      121 gcagccgagc ggtgcgatga agcgcccgtc ggaggtgccg ccggtggtgg agagctcggg
      181 cgtgggcgcg cccagctccg cgatgcccgc caccgcgctg tcgagcagct cgccgcgcgc
      241 ggtgaggaag ggctcgccgt gcagctgcca cgcgatgtcg tgatcgatgc cgtggcgcgc
      301 gagcagggca gtgatgcgcg acttcagcgt cgcggcgtcg gactcagtgc tgaagcgcag
      361 gttgaacacc acgtacagct cgccgggaat cacgttggtg gcgccctggc cggcgtggat
      421 gttggagatc tgcagcgagg tcgcggggaa atgcgcgttg ccttcgtccc agcgcgtggc
      481 cacgagctct gcgagcgcgg cgagcgcctc atggatcggg ttgcgtgcgc gctcggggta
      541 ggccacgtgg ccctgcacgc cgcgcaccgt gagctcggcg ccgagcgagc cgcgccgacc
      601 caccttgatc acgtcgccga cgcggtgcgt gctggagggt tcgccgacca cgcaccagcg
      661 gatctgttcg ccgcgctcgc gcaaccactc gaccacgcgt cgcgtgccgt cgatcgaggg
      721 gccttcctcg tcgctggtga gcaggaaggc gatgcgcccc tgtaccgccc cggtggccag
      781 cagccgttcg caggccgtga ccatcgccgc gaggctgccc ttcatgtccg cggcgccgcg
      841 cccgtgcagc atgccgttct cgatgcgcgg ctcgaagggc ggatgcgtcc acgcggccag
      901 cggtccgggc ggcaccacgt cggtgtggcc cgcgaacacg aacagcggtc cctcgccgcc
      961 gcgcacggcc cagaggttgt cgacctcgcc gtaacgcagg tgcgtgcacg cgaaacccat
     1021 ggcctccagg cgcgcggcga gcagctgctg acagccggca tcgtcgggcg tcaccgaggg
     1081 gcgggcgatc agttcggcgg cgagcgccag cgtcgcggaa ggccaggcgg gctgcgtcat
     1141 gctcagttgt gcgcgtgcag ggcttcgttc aacgcgatcg ccgcgcggtt gctcagacac
     1201 tcgatcgcgc cgctctgcga gttgcggcgg aacagcatgt cggagccgcc gctcagttcg
     1261 cgtgccgcga ccaccttcac gaccgcgccg gcctcgtcga tcaccgtcac gcgcgagccg
     1321 gccgtcacgt agaggccggc ctcgatggtg caacggtcgc cgagcgagat gcctatgccc
     1381 gcgttggcgc cgatgaggca ttcgcgtccc accgcgatga gcgccttgtt gccgcccgag
     1441 agcgtgccca tggtcgagca gccgccgccc agatcggaac ccgcgccgac gaagacgccg
     1501 gccgagatgc ggccctcgat catgccggga ccttcggtgc cggcattaaa gttcacgaag
     1561 ccctcgtgca tgatcgtggt gccttcgccg agccaggccc cgaggcgcac gcgcgcggca
     1621 tcggcgatgc gcacgcccgc cggcaccacg taatcgctca tgcgcggaaa cttgtcgacg
     1681 ccgtgcacgg tgagcgtgcg gcccgccacg cgcgcggcga gctggcgcgc gggcagctcg
     1741 gcgacctcga tcgcgccctc gctggtccag gcgacgttcg gcagcaccgc gaacaggccg
     1801 tcgagcttct gctcgtgcgg ccggcacagg cggtgcgaga gcaggtgcag cttcaggtag
     1861 gcctccggca cgccggccgg cgcggcgtcc gtggccagca ccgtgatcac gggcgcaccc
     1921 cggccgccgg cgagctgtcc ggcgatcgcg gcctgcgcct cgtggcccgc ggccccgagc
     1981 gccgtggcga gcgccgccag ctgctgcgct gagggttcga gcgccgcgtt gccgccggcg
     2041 tagccgcagt gcgtggcgag cgcggcggcg agggcggcgt cgacgttgcg cagcggcgcg
     2101 ggatagaaca cctcgagcca gttgccgcgc gccccgcgcg taccggttcc gagtccaatg
     2161 gcaaacagcg gcgctgtcat gggcgatgca atctccgggg caggttgtca ggaacgggag
     2221 aacagcagct cgtagtcggt cgggttgaaa ccgcagtgga gcaggccgtc acccgcgagt
     2281 accgggcgct tgatcagcgt cgggttggcg atgagcacgg gaacggggtt gcccgactcg
     2341 atctgctcgc gctggctgcg ctcgagcgtg cgccaggtgg cgctggactt gttcaccagg
     2401 cgctcgcgcc cgagcgcggc gagccattgc tcgagcgtct gcgcatccac gtgttccttg
     2461 cgcaggtcgt ggaactcgtg ggcgatgccg tgctccttca gccagcggcg cgcggcgctg
     2521 acggagtcgc agttggcgat gccgtacacg ttcaacacgg attgtgctcc agcgggtgga
     2581 cgaagggggc cgagcttagc aaatgcgggc ggcgcagacc acgctcgggg ggctgacgcg
     2641 ggtaatcctt gtgctatagt tgccggcatt cacaggacgc ccagaaaccg cgtgcgcccg
     2701 gagggcaatc ccgcatgtcg atgctaccga aggtggaaat tttcgccggc ctctcggagg
     2761 aggagcaggc cgcgctcgag aattcctgcg tcacgcgcaa ctatccgcgc aacaccgtgg
     2821 tcatcaacga gaatgacttc gccgattcgc tgtacgtgat cgagagcggc cgcgtgaagg
     2881 tctattgcag cgacaagaat ggcaaggaat acatcatgaa cacgctgggg ccgggggact
     2941 acttcggcga gctggcgctg ctcgacgacg accgccgttc cgcctcggtg cgcacgctgg
     3001 agaagtcgac cttcacgatc atgttcaagg acgagttcca cgcgatcatg gagcgccatc
     3061 cgaacatcgc cacgaccctg atccgcaacc tcacgcggcg cgtgcgcaaa ctcaccgaga
     3121 acgtgaagag cctggccctg caggacgtct acggccgcgt caccaaggtg ctgatgacgc
     3181 tcgccgagcc gcgcggcgac aagctctaca tcgaggagcg cctgacgcag caggacatcg
     3241 ccgatcgcgt cggcgcctcg cgcgagatgg tcgcgcgcat cctgaaggat ctggccatcg
     3301 gcgactatat cgccttcgag ggcaagaaca tcatcatcaa cggcaagctg ccgaccagtt
     3361 actgagggtc ttccgtggcg ggctcccggg cccgcccggc ccacggcatt attcacggtt
     3421 ccccccgaag ttcccccaga cgggccggtg atatattcac ccgcccgtga ccgcaaagcc
     3481 tttgcggccc gcaacacccc ggacccctat gagcggacgt cgaccccagg ccgcctggtg
     3541 gcaggagctc taccgcagcg agcgcttcaa cggcgcagaa cggttgctgc tcggtgttgc
     3601 ggcactggcg ctgaccgggg cgctggtggt gctggtgcgc gtcgtgagct gaggccgcgc
     3661 ctcagtcctc ctcgataccg aacacctcga tcaggctctc gcacagctgg tcgagctcca
     3721 tcgacatcag cgcgaagtcg gcgtcgaaac gcgccgctgc ttccatctcg tcctcggccc
     3781 cggcgtcctt ctgcacgatc tcgctgtact tgatcgcttt caccgcgaga tcctcggcca
     3841 gcacgaaccc gatgcgctcg ttccattcga gccccagcgc gctgacctgc ttgccggcgt
     3901 cgaggtgctg gcgcacctcc tgggtcgcga gctcctgctg gcggcagcgc accacgttcg
     3961 cggcgttgag cggatcgcgc atgtcgcact gctgtccgag acgatagccc ggcgggaggc
     4021 gcccgccgtg cagccagcgc gtcatcaggt cgacggggct gtggcgcggc accagcggct
     4081 tgaccggcag gcgacccagg ctgtcgcgca gcagattgag caggtcctcg gcgcgtgccg
     4141 gcgaggagga atcgatcagc agcaggccgc ggctctcggc caggaaggca taggtgaggt
     4201 gcgagcgcgt cagcgcgcgc ggcagcagcg tctggatcac ctcgtccttg aggttggcgc
     4261 gctcgcgttt gcgcagttcg cgcccctcgg cggcctcgat ctcgaggatc ttctcgtcca
     4321 gcacctcgcg gatcgcggcc gccggcagca gtcgctgctg cgtgcgggcg catacgaggt
     4381 ggaagttcgc ggcgctgtgc accaggtgtg tcgccgtcgg gcccatcggc gagacccagc
     4441 ccgagcgctg cgtgtcgagc ccgccgcagg gcacgaagct cgttgccagc agccgctcgc
     4501 cgagctcatc gacggggcag gggagcaggt cggtgaggcg gtagatgcgc agattcttga
     4561 accacatcgg gggagccagg ccagtcaggg ggggcggatt atggcggaag ccatgacgaa
     4621 tggccacgtg caattgcggg ggcggcaaag gccgctacaa tgcagccaga catcgcctcg
     4681 cggggaagct caccgttgct cgaactcatc aaatccggcg gctgggtgat gctgcctatc
     4741 atcctgggct cgatcgccgc cgtggcgatc agcatcgaac gctggtggac gctcgactac
     4801 cgcaaggtgg tgccgccgaa cacgctggcc gaagtctggc agctgatccg ccgcaacgag
     4861 ctctcgcccg agcgtctgcg cgcgctcaag gcctcctcgc cgctggggcg catcctggcc
     4921 gccgggctgg cgaacgccca cgccgggcgc gacgtgatga aggagagcat cgaggaagcc
     4981 gcgagccacg tgatccacga cctcgagcgc tacctgaact cgctgggcac cattgccgcg
     5041 atcgagccgc tgctcggcct gctcggcacc gtgctcggga tgatccaggt gttctccgac
     5101 gtgatgctgc acggcacggg caatgcggaa ctgctggccg gcggtatctc gaaggcgctg
     5161 atcaccaccg cggccggcct gatcgtcgcg attcccgcgc tgatgctgca ccgccacttc
     5221 ctgcgccgcg tcgacaccat cgtggtcggt ctcgagcagg aggcgatcaa gctggtcgat
     5281 gccatgcacg gcgggcgtcc ggtcgatgtg cgcgagaagc cgggcacgtg aagttccgcc
     5341 gcaggcaacg cgtcgaggtc gggctcgaca tcacgccatt gatcgacgtg gtgttcctga
     5401 tgctgatctt cttcatggtc tcgacgacct tcacgcgcga ggcgcacctg cagatcagcc
     5461 tgccggaagc cgatgccgcg ccggccctcg acgagaagga cagactcgaa ctgatcgtcg
     5521 ctgcttcagg ctcctacgcg ctcaacggca gggtgctggt gaattcgcgc atcgagacgc
     5581 tgctggccgc gctgggcgaa gcctccggcg gcgacgtgac gcagccgctg gtgatcacgg
     5641 ccgacgcgaa gacgccgcac gaactcgtgg tgcgcgccat ggacgcggcc ggtcgcctcg
     5701 gtttctcgcg cctcagcatc acgacgcggc tgcctcagga gggcggctga gcttttccgt
     5761 gctccgtcga tccgcgccat attccccgag gaacccagcg tgacattcag cgtaatcatt
     5821 cccgcgcgat tcgcctccac ccggcttccc ggcaagccgc tgctcgacat tgccgggcag
     5881 ccgatgatcc gccacgtctg ggagcgcgca tgccgcagca gcgccgcgcg cgtggtgatc
     5941 gcgaccgacg acgagcgcat tctcgaagcc gcgcgcgcat tcggcgccga ggtctgcctc
     6001 acgggcgccc accaccagtc cggcaccgac cggctgcagg agaccgtcgc gacgctgggc
     6061 ctcggcgccg gggagatcgt ggtgaacgtg cagggcgacg agccgctgat tccgccggct
     6121 gtgatcgacc aggtcgcggc gaatctcgcc gcgaaccgcg cgcacggcat cgcgacgctg
     6181 tgcgaggaga tcaccgcgat cgggcacttc ctcgatccca acgtggtgaa ggtggtgtgc
     6241 gccgaggacg gcagcgcgct ctatttcagc cgcgcgccga ttccgtggcc gcgcgatgcc
     6301 ttcgcgcgct cgcgcgagga gatgcccgcg ggtctgcgcg cgtaccgcca tatcggcatc
     6361 tatgcctacc gcgtggattt cctgaaccgc ttcgtcggct ggccggtcgg cacgctggaa
     6421 ggtttcgaat cgctggaaca actgcgcgcg ctcgagcacg gggtaggcat ccacgtcgcg
     6481 caggcctgcg cgccgatttc cggcggcgtg gacacgcccg cggacctcgc tcgcgtcgat
     6541 gcgctgttgc gtggcgatgg ctgaagatcg cggcggctgt cggtccgtga gccgggccgg
     6601 catgtattgt ctatgggccg gcaggcgatc atggtaaaca cccggattcg ccgtaccgca
     6661 tcccggcggc acgacagcga ggaggaagcg tcatggcgcg ggttcaggat ttcttgcggc
     6721 gccaccatgg tgtgatggat tatcaccctg cgtgggaatc cggggcgcac ctcgccaatc
     6781 gccggcggct ggtcgtgttc accggcgtgt tcgccgtgct ctgc
//
LOCUS       DAORUQ010000166         6758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_13019_length_6758_cov_6.158586, whole genome shotgun sequence.
ACCESSION   DAORUQ010000166 DAORUQ010000000
VERSION     DAORUQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13019_length_6758_cov_6.158586"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLW13_12780"
     CDS             <1..895
                     /locus_tag="PLW13_12780"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY71300.1"
                     /translation="GLDMTDEMLELAERNRAEAGIDNAHFVKGTIENIPLPDAIVDVV
                     ISNCVINLSTDKDRVLAEAFRVLRPGGRFAVADVVLLRDLEPRLMTVVELWPGRGWYT
                     EVLAPYLRDKGKLYAAHFPAQSNVEYYQKGLAAFKAMLAANPALYDRVVLTELGPPDS
                     LAVAPPASADAVLTFRNMHNWMGQGNDVKVMQAAFAALKPGGVLGVVEHRAAANATAE
                     QVKDSGYMTEEMVIKAAEAAGFVLEAKSEINANPRDTRDHPKGVWTLPPSLALGDTDR
                     EKYVAIGESDRMTLRFRKPTQ"
     gene            892..1152
                     /locus_tag="PLW13_12785"
     CDS             892..1152
                     /locus_tag="PLW13_12785"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HQY71301.1"
                     /translation="MTALEIPWRELSADALQGVIEEFATREGTEYGLSEVPLATKVAQ
                     IRRQLERGEVLVFFDTQLESCQLLTREQAAQLRAALATEPGA"
     gene            1149..1385
                     /gene="tusA"
                     /locus_tag="PLW13_12790"
     CDS             1149..1385
                     /gene="tusA"
                     /locus_tag="PLW13_12790"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HQY71302.1"
                     /translation="MSAPVESTLDTRGLYCPEPVMLLHNRIRDMAPGDVVVVLASDPS
                     TQRDIPRFCEFLGHALLAHEEPDGEFRYRIRKGG"
     gene            1390..1950
                     /locus_tag="PLW13_12795"
     CDS             1390..1950
                     /locus_tag="PLW13_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HQY71303.1"
                     /translation="MPRPSPDCDALCALFDELFLASENTRLVKGGDEPVYLPANAECP
                     EHRIVFRLDYAPSALHEIAHWCIAGAQRRLLPDYGYWYSPDGRNAAAQREFERLEARP
                     QALEWLLNEACGLRFRPSIDNLDGAAGDTRAFAAAIAREAAAYCVNGLPPRAARLHAA
                     LAARFGGRAVLRPGDYALQRLLEGGG"
     gene            1953..2849
                     /locus_tag="PLW13_12800"
     CDS             1953..2849
                     /locus_tag="PLW13_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HQY71304.1"
                     /translation="MQPHYLLDASIYIFRAYFALAPGWRAANGLPTHAVQGYAGFLCD
                     FLRRAAPRHVLAAFDESLGSCFRNQIHPGYKCSRALPDAALEFQLRACRELTGLLGIA
                     SVASARFEADDLIASAACAARGQGDCAVVISRDKDLGQVLRGADDRLWDFPAAAPLDR
                     ATYRARHGIAPEQVPDLLALCGDPVDDVPGVPGIGVKTAVALLQRHPDVHALLGALDT
                     IAASSLRGAARIARLLEAHGEQILMARRLTALAEDAFATQELPASARQPAQRAALAHF
                     ADFHGLGERLRARLLAVADAAT"
     gene            2846..3973
                     /locus_tag="PLW13_12805"
     CDS             2846..3973
                     /locus_tag="PLW13_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HQY71305.1"
                     /translation="MNLLIDENIPGAREAFARFGSVRLMAGRAIDAAAVRDADALLVR
                     SVTRVDRALLAGSRVRFVATATAGTDHVRIAELDALGIRFAHAPGSNADSVVDYVLGV
                     LALAYPAPRGLAGRTVGIVGCGQVGARLLVRLRALGVACRVYDPLLTRDIPPESCALQ
                     ETLACEVLSLHVPLTREGAHATRHLLGARELARLRDDALLINAARGPVVDNAALLEAM
                     RRRGALRAALDVWEHEPQLDRALLARVLIGTPHIAGYAHDGKLRGARQVYAALRAFLG
                     AGASPADPSELRPAPAGVLRAGDCGSWQQAVLACYDPRGDDARLRAAMLDEDEGARAA
                     AFDRLRRDYPLRREFSAWRIGTAAAPGTPVHAALLAAGFAL"
     gene            3985..5130
                     /locus_tag="PLW13_12810"
     CDS             3985..5130
                     /locus_tag="PLW13_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HQY71306.1"
                     /translation="MSSGQAFRLGLIVNPLAGIGGAVGLKGSDGAAIVAEARERGAEP
                     RAHARAVRALRMLAPLAGRIRIHAGAGAMGEDAAREAGFAVELAGGAIPAQTCAAHTV
                     ALARALAALPVDLLLFAGGDGTARDICGAIGTAVPALGIPAGVKMHSGVYAVSPEAAG
                     EIVAQLVAGTLVRVAPGEVRDLDEDAFRAGRVSARHFGELLVPEESRYVQHVKQGGVI
                     ADESAVAEIAAEMTQSMEPGCLYILGPGTTTFAIKQALGIAGTLLGVDVVRDGQCLAR
                     DAAEAQLLALLAAHAGPAAIVVTAIGGQGHVFGRGNQQISARVIRAVGIANITIVATR
                     HKIAALGGRPLLADTNDANLDRELHRYLRVVTGYREAILYRVGYDTP"
     gene            complement(5165..6382)
                     /locus_tag="PLW13_12815"
     CDS             complement(5165..6382)
                     /locus_tag="PLW13_12815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQY71307.1"
                     /translation="MREYLKSHKLANVCYDIRGPVHARAAAMEDEGHRIIKLNIGNPA
                     PFGFEAPDEILIDVIHNIPNSQGYSDSRGLYSARKAVMQYAQQLRIPNVEVNDIYLGN
                     GVSELIVMVTQALANTGDEILIPAPDYPLWTAATTLAGGTPVHYRCDEQSDWYPDLAD
                     IRAKITDRTRAILVINPNNPTGAVYPREILEGIVQIAIEHGLVILADEIYDKILYDDA
                     QHTALASISHEPLCITFNGLSKTYRLAGFRSGWMIISGAKHKARDFIEGLDILSTMRL
                     CANVPAQHAIQTALGGYQSINDLVLPGGRLREQRDKAWEMINAIPGVSCTKPKGAIYM
                     FPRLDPKVHRILDDEKMIFDLLTQEKMLLVQGTAFNYPDPDHFRIVTLPLVADLEEAI
                     GRLGRFLQTYSQV"
     gene            6525..>6758
                     /locus_tag="PLW13_12820"
     CDS             6525..>6758
                     /locus_tag="PLW13_12820"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020319446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HQY71308.1"
                     /translation="MSTEKTEEQWREQLTPEQFNICRGKGTERAFTGKYWDTKTPGTY
                     RCACCGEALFDSATKYDSGSGWPSFYAPVAEAAL"
BASE COUNT          945 a         2325 c         2448 g         1040 t
ORIGIN      
        1 cggcctcgac atgaccgacg agatgctgga gttggcggaa cggaaccggg ccgaagccgg
       61 catcgacaac gcccacttcg tcaagggcac catcgagaac atcccactcc cggacgccat
      121 cgtcgacgtg gtcatctcca actgcgtgat caacctctcc accgacaagg accgggtgct
      181 ggccgaggcg ttccgggtgc tgcgcccggg cggccggttc gccgtcgccg acgtcgtgct
      241 gttgcgtgat cttgagccgc gcctgatgac cgtggtcgag ctgtggccgg gtcgcggctg
      301 gtacaccgag gtgctggcgc cgtacctgcg cgacaagggc aagctctacg cggcgcattt
      361 cccggcgcag agcaatgtcg agtactacca gaagggcctc gcggcattca aggcgatgct
      421 ggcggcgaac ccggcgctct acgaccgcgt cgtcctcacc gagctcgggc cgcccgacag
      481 cctcgcggtc gcgccgccgg caagcgcgga tgcggtgctc accttccgca acatgcacaa
      541 ctggatggga cagggcaacg acgtcaaggt catgcaggcc gccttcgcgg cgctgaagcc
      601 cggtggcgtg ctcggcgtgg tcgagcaccg tgccgcggcg aacgccactg ccgagcaggt
      661 caaggacagc ggctatatga ccgaggaaat ggtcatcaag gcggccgagg ccgcgggctt
      721 cgtgctcgag gcgaagagcg agatcaacgc caacccgcgc gacacacgcg atcaccccaa
      781 gggtgtgtgg acgctgccgc cctcgctcgc gctgggcgac acggaccgcg agaaatacgt
      841 cgcgatcggc gagagcgacc gcatgacgct gcgtttccgc aaaccgacgc aatgacggca
      901 ctggagatcc cgtggcgcga actctccgcc gatgcgctcc agggcgtgat cgaggagttc
      961 gcgacccgcg aaggcaccga gtacggcctc tccgaggtgc cgctggccac caaggtcgcg
     1021 cagatccgcc gccagctcga gcgcggcgag gtgctggttt tcttcgacac gcagctggag
     1081 agctgccagc tgctgacccg cgaacaggcc gcgcagctgc gcgcggccct cgccacggaa
     1141 cccggggcgt gagcgctccc gtcgaatcca cgctcgacac gcgcggcctg tactgtcccg
     1201 agccggtgat gctgctgcac aaccgcatcc gcgacatggc gcccggcgac gtggtcgtgg
     1261 tgctcgccag cgatccctcg acgcagcgcg atattccgcg cttctgcgaa tttctcggcc
     1321 acgcgctgct cgcgcacgag gagccggatg gcgaattccg ctaccgcatc cgcaagggcg
     1381 gctgaacccg tgccgcgccc gtcgcccgat tgcgacgcgc tgtgcgcgct gttcgacgag
     1441 ttgttcctcg ccagcgagaa cacgcgcctg gtgaagggcg gcgacgaacc ggtctacctg
     1501 cccgccaacg ccgaatgccc cgagcaccgc atcgtgttcc gcctcgatta cgcgccgagc
     1561 gcgctgcacg agatcgcgca ctggtgcatc gcgggcgcgc aacgccgcct gctgcccgac
     1621 tatggttact ggtacagccc cgacggccgc aatgccgcgg cgcagcgcga gttcgagcgc
     1681 ctcgaggcgc gcccgcaggc actcgagtgg ctgctgaacg aggcctgcgg cctgcgtttt
     1741 cgcccgagca tcgacaacct cgacggcgcc gcgggcgata cgcgcgcctt tgccgcggcc
     1801 atcgcgcgcg aggccgcggc gtactgcgtc aacggcttgc cgccgcgcgc ggcacgcctg
     1861 cacgcggcgc tcgcggcgcg cttcgggggc cgcgccgtgc tgcgccccgg ggactacgca
     1921 ttgcagcggc tgctcgaggg cggcggctga cgatgcagcc gcactacctg ctcgacgcct
     1981 cgatctacat cttccgcgcc tacttcgcgc tggcgcccgg gtggcgcgcc gccaacgggc
     2041 tgcccacgca cgcggtgcag ggctacgcgg gtttcctctg cgatttcctg cgccgcgccg
     2101 cgccgcggca cgtgctcgcg gccttcgacg agagcctcgg cagctgtttt cgcaaccaga
     2161 tccaccccgg ctacaagtgc agccgcgcgc tgcccgacgc ggcactcgag ttccagttgc
     2221 gcgcctgccg cgagctcacc ggattgctcg gcatcgcgag cgttgccagc gcgcgcttcg
     2281 aggccgacga cctgatcgcc tccgcggcct gcgcggcgcg cgggcagggc gattgcgcgg
     2341 tggtgatcag ccgcgacaag gacctcgggc aggtgctgcg cggggcggac gaccgcctgt
     2401 gggatttccc cgcggccgca ccgctcgatc gcgcgaccta tcgcgcgcgc cacggcatcg
     2461 cgcccgagca ggtgccggat ctgctggcgc tgtgcggcga tccggtcgac gacgtgcccg
     2521 gcgtgccggg catcggggtg aagacggctg tcgcgctgct gcagcgccac cccgacgtgc
     2581 acgcgctgct cggcgcgctc gatacgattg ccgcctcctc gctgcgcggc gcggcacgca
     2641 tcgcgcggtt gctggaagcg catggcgaac agatcctgat ggcgcggcgc ctgaccgcgc
     2701 tcgccgagga cgcattcgcg acgcaggaac tccccgccag cgcgcgccag cccgcgcagc
     2761 gcgccgcgct ggcgcacttt gccgactttc acggcctggg cgagcgcttg cgcgcgcgcc
     2821 tgctggccgt cgcggacgcc gcgacgtgaa cctgctgatc gacgagaaca ttcccggcgc
     2881 gcgcgaggcc ttcgcgcgct ttggcagcgt gcgcctcatg gccgggcgcg cgatcgatgc
     2941 cgccgccgtg cgcgatgccg acgcgctgct ggtgcgctcg gtcacgcgcg tcgatcgcgc
     3001 gctgctggcg ggcagccgcg tgcgcttcgt cgcgaccgcg accgcgggca cggatcatgt
     3061 gcgcatcgct gagctcgacg cgctcggcat ccgcttcgcg cacgcgccgg gcagcaacgc
     3121 ggattccgtc gtcgattacg tgctgggcgt gctcgcactg gcgtacccgg cgccgcgggg
     3181 actggccgga cgcacggtcg gcatcgtggg ttgcgggcag gtgggcgctc ggctgctcgt
     3241 acgcctgcgc gcgctcggcg tggcgtgtcg cgtgtacgac ccgctgctca cgcgcgacat
     3301 tccgccggaa tcctgcgcgc tgcaggagac gctggcctgc gaggtgctga gcctgcacgt
     3361 gccgctgacg cgcgagggcg cgcacgccac gcgccacctg ctcggcgcgc gggaactggc
     3421 gcgcctgcgc gacgatgccc tgctgatcaa cgccgcccgc ggccctgtgg tggacaacgc
     3481 ggcactgctc gaggccatgc gccggcgagg ggcgttgcgt gccgcgctgg acgtgtggga
     3541 gcacgagcca cagctggatc gcgcgctgct ggcgcgggtg ctgatcggca cgccgcacat
     3601 cgccggctat gcgcacgacg gcaagttgcg cggcgcgcgc caggtctacg cggcgctgcg
     3661 cgccttcctc ggcgccgggg cctcgcccgc cgatccgtcc gagctgcgcc cggcgccggc
     3721 cggcgtgttg cgggccgggg actgcggctc gtggcagcag gcggtgctcg cctgctacga
     3781 cccgcgcggc gacgacgcgc gcctgcgcgc cgcgatgctc gacgaggacg agggcgcgcg
     3841 cgccgccgcc ttcgaccgcc tgcggcgcga ttacccgctg cggcgcgaat tctccgcttg
     3901 gcgcatcggc acggccgcgg cgcccggcac gccggtgcac gcggcactgc tcgcggcggg
     3961 cttcgccctg tgaacgaggt cgaaatgtcg agcgggcagg cgtttcgcct cgggctgatc
     4021 gtgaatccgt tggccggcat cggcggcgcc gtggggctga agggcagcga cggtgccgcg
     4081 atcgtggccg aggcgcgtga gcgcggcgcc gagccgcgcg cccatgcccg cgccgtgcgc
     4141 gcgctgcgca tgctcgcgcc gctggccggg cgcatccgca tccatgccgg cgccggtgcc
     4201 atgggcgagg atgcggcgcg cgaggccggc ttcgccgtcg agctggcggg cggcgcgatt
     4261 cccgcgcaga cctgtgcggc gcacaccgtc gcgctcgcgc gcgcgctcgc ggcgctgccg
     4321 gtggacctgc tgctgttcgc cggtggcgac ggcaccgcgc gcgacatctg cggcgccatt
     4381 ggcaccgcgg tgcccgcgct cggcattccc gccggcgtga agatgcactc gggggtctac
     4441 gcggtctcgc ccgaggcggc cggcgagatc gtggcgcaac tcgtcgccgg tacgctggtg
     4501 cgtgtggcac cgggcgaggt gcgcgatctc gacgaggacg cctttcgcgc cgggcgtgtc
     4561 agtgcgcgcc acttcggcga gctgctggtg cccgaggaaa gccgctacgt gcagcacgtg
     4621 aagcagggtg gcgtgatcgc cgatgagagc gcggtcgcgg aaatcgcggc cgagatgacg
     4681 cagagcatgg agcccgggtg cctgtacatc ctcggccccg gcaccaccac tttcgcgatc
     4741 aagcaggcgc tgggcatcgc gggcacgctg ctcggtgtgg acgtggtgcg cgacgggcag
     4801 tgccttgcgc gcgatgccgc cgaggcacaa ctgctggcgc tgctcgcggc gcacgccggc
     4861 ccggcggcca tcgtggtcac cgcgatcggc ggccaggggc atgtgttcgg tcgcggcaac
     4921 cagcagatca gtgcgcgcgt gatccgcgcc gtgggcatcg ccaacatcac gatcgtcgcg
     4981 acccgccaca agatcgccgc gctcgggggg cgcccgctgc tggccgatac caacgacgcc
     5041 aatctggacc gcgaattgca ccgctacctg cgcgtggtca cgggataccg cgaggcgatt
     5101 ctctaccggg tcggatacga caccccgtag gtcgccatga atggcgaccc acggggcctg
     5161 gcgatcagac ctggctgtag gtctgcagga agcgcccgag acgtccgatc gcttcctcga
     5221 ggtcggccac cagcggcagc gtcacgatgc gaaagtgatc cgggtcggga tagttgaagg
     5281 ccgtgccctg caccagcagc atcttctcct gcgtcagcag gtcgaagatc atcttctcgt
     5341 cgtcgaggat gcggtgcacc ttcgggtcga ggcgggggaa catgtagatc gcccccttgg
     5401 gcttggtgca gctcacgccc gggatcgcgt tgatcatctc ccaggccttg tcgcgctgct
     5461 cgcgcaggcg cccgccgggc agcaccaggt cattgatgct ctggtagccg ccgagcgcgg
     5521 tctggatcgc gtgctgtgcc ggcacgttgg cgcacaggcg catcgtggac aggatgtcca
     5581 gcccctcgat gaaatcgcgc gccttgtgct tggcgcccga gatgatcatc cagcccgagc
     5641 gaaagccggc cagtcgatag gtcttcgaca gtccgttgaa ggtgatgcac agcggttcgt
     5701 gggagatgct ggcgagcgcg gtgtgctgcg cgtcgtcgta gaggatcttg tcgtagatct
     5761 cgtcggcgag gatcacgagg ccgtgctcga tggcgatctg cacgatgccc tcgaggatct
     5821 cgcgcgggta caccgcgccg gtggggttgt tggggttgat caccaggatg gcgcgcgtgc
     5881 ggtcggtgat cttggcgcgg atatcggcca gatccgggta ccagtcggat tgctcgtcgc
     5941 agcggtagtg aaccggcgtg ccgccggcca gcgtagtggc ggcggtccac agcgggtagt
     6001 cgggggcggg gatgagaatc tcgtcgccgg tgttggcgag cgcctgcgtc accatcacga
     6061 tcagctcgct gacgccgttg ccgaggtaga tgtcgttgac ctcgacgttg gggatgcgca
     6121 gctgctgggc gtactgcatc accgccttgc gcgcggaata gagtcccctg gagtcgctgt
     6181 agccctgcga attcgggatg ttgtggatga cgtcgatgag gatctcgtcg ggagcctcga
     6241 agccgaaggg ggcagggttg ccgatgttca gcttgatgat gcgatggccc tcgtcctcca
     6301 tcgccgccgc gcgcgcatgt accggaccgc ggatgtcgta gcagacgttg gccagcttgt
     6361 gggacttcag gtattcgcgc atcggtcggg ttctggagcg tgattcgggg cgctagtata
     6421 ggcgatgggt cccggcgctt cctatggcgc aggccgcgca gcggggctgc ggcggggcga
     6481 ttcgcgcatt tgtgcacaac agcggatgga ggagggtcga ggcaatgagc acggagaaga
     6541 ccgaggagca gtggcgcgag caactcacgc cggagcagtt caatatctgc cgcggcaagg
     6601 gcacggagcg cgccttcacc ggcaagtact gggacacgaa gacgccgggt acctatcgct
     6661 gcgcctgttg cggcgaggcg ttgttcgatt ccgccaccaa gtacgactcg ggttccggct
     6721 ggccgagctt ctatgccccg gtagccgagg cggcgctg
//
LOCUS       DAORUQ010000167         6554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_13650_length_6554_cov_6.008155, whole genome shotgun sequence.
ACCESSION   DAORUQ010000167 DAORUQ010000000
VERSION     DAORUQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13650_length_6554_cov_6.008155"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLW13_12825"
     CDS             <1..1045
                     /locus_tag="PLW13_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQY71309.1"
                     /translation="PEPGGEMSGVLCFTSINYHYLAKARVLASSLKRFHPDWHFVLCV
                     TDAMPDGGTLDLADEPFDELLITQEVLGDLGPGWFFKHDVVEVCTAVKGPLLEKFCDR
                     GFDAIVYLDPDIAVFNPLQGLIEALERSDILLTPHQLEPDDQYYQIVDNEVCSLRHGI
                     FNLGFVAIKPSAEGRRFARWWSARLTDFCVSDERLGLFVDQKWCDHVPVFFDGVKILR
                     DPGYNVASWNLSRRKISIDRSGRILCNRKLLYFYHFTKLGLVGDAMTQRYAKDNIEVY
                     ELWSWYRRKVEDFAFDDLPDGWWALGQFSNGAKITSEQRRCYRDRPDLQTYFPDPYLA
                     EGNSFYEWCGRNA"
     gene            1116..1658
                     /locus_tag="PLW13_12830"
     CDS             1116..1658
                     /locus_tag="PLW13_12830"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71310.1"
                     /translation="MNGCAWKERVFVLGDSRTGTSSFHQLFLLAGLKSIHFYEEEAGM
                     LPHVAGNHAENSRRLLRYIGNSGIQCFSDYPTRLYYKELHAAYPEALFILTKRVNVKV
                     WCESMLNYFDHITDIDELAFHYLRINADISRYFAASGNFCEIAIDAPGFAGAFCRIKR
                     HLRIDGDVEFPHVNRWQDKE"
     gene            1739..3028
                     /locus_tag="PLW13_12835"
     CDS             1739..3028
                     /locus_tag="PLW13_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQY71311.1"
                     /translation="MLRDPNIVAVDLTPVLPGGENGGAKVFVLELLRGLAAQAPQMRF
                     VLLTRVETHDELAVLDGPNVERRLVLGAAQPTPARPALWVRVAHRLARYLPGRIRALA
                     SRAGYRLMRHRRRAAAPGLLQGIGAKLLFCPFTAPTYAEPGVPVVSVVHDLQYLSYPQ
                     FFEAADIGHRHQVFRAACNEATLLVAISDYARDSVIRQGAIAPDRVRTIHHRLGKRLG
                     SAAGADESVLARLGLARGRYLVYPANFWKHKNHEMLLTAFGIAAHGGLPQDMRLVCTG
                     APGARRDFLAQAADAMGLGSRVVFPGYVPTEALAALLGNSAGMVFPSLYEGFGMPVIE
                     AMAAGVAVACSNATSLPEIATGAALLFDPRIPGQIAAAMLELVNDPARRADLVAAGLE
                     RAAGFSDAGRMADEYRDAFRQAMGQALPRPGRGGAGA"
     gene            3025..3891
                     /locus_tag="PLW13_12840"
     CDS             3025..3891
                     /locus_tag="PLW13_12840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY71312.1"
                     /translation="MKVSVITPSFNQGRFIERSIRSVATQGVAELEHVVMDGGSTDET
                     RAVLERCGATVRWVSEKDRGQTDALNKGIRATNGEIIGWLNSDDVYYPGAIARVLEVF
                     RTQPGVDVVYGMADHIDIDDRAFEEYPTEAWDLERLKETCFICQPALFLRRRVVEQHG
                     LPDESLHYCMDYEYWLRLGKGGARFHYLEEKLAGSRLYGENKTLGARVAVHREINDMM
                     RRHFGRVPERWLFNYAHAVVEPRVERARAPRRFVLCLALHSIAAALRWNHGIGPAMRR
                     VLWHWGRDAYRL"
     gene            3875..5032
                     /locus_tag="PLW13_12845"
     CDS             3875..5032
                     /locus_tag="PLW13_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQY71313.1"
                     /translation="MHIGFDISQTGAGKAGCGYYADAMLQALLRLAPAHRFSLYPGFG
                     DFYFDARMPLRNPYPGANAEYGPRHLTRESAAEFWNAPALADALGRPDIVHANNFWCP
                     PRLEGSRLVYTLYDLAFLVDPSWTTEANRIGCFEGVFRSSIAADWVVAISQASRGDYL
                     RTFPHFPAERVRVIHPCSRFFAADDLPGERHERLRHVAPGRFWLSVGTIEPRKNQRRL
                     AEAFAGYLAGGGEPMPLVLAGGEGWRMEGFPLFLEELGIAEHVIVTGYVTDAELIWLY
                     RNCYANLYPSLFEGFGLPVLEGMQFGAPTIASCSSSIPEVAGEAAVLLAPEDARAWTR
                     AMLELAAHPVQRDAMAGRSRERAACFDWNRSAGQLLALYDEALSQPARSRT"
     gene            5029..6042
                     /locus_tag="PLW13_12850"
     CDS             5029..6042
                     /locus_tag="PLW13_12850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY71314.1"
                     /translation="MKRALITGITGQDGSFLAEFLLARGYAVYGLARRESWFRANNAS
                     HLAGRVEILFGDMTEGIDIASAVQDARPDEIYNLASQSRPGESWARAPETLMINGMAA
                     IRLFETVRHACPAAKVYHASSSEMFGRTTGVAQDEATPFNPQNPYAAAKVYAHQMARI
                     YRESYGMFIATGILFNHESERRPLHFVTQKIAYGAACAALGIGDSPDLNERGRPIVEH
                     RHLALGNLEVARDWGHARDFVRAMWLILQQGTADDFVVGTGIVHTLRDLCDAAYAHVG
                     LDWREHVVSDPALVRPLETGRTVANPARARARLGWEPEIGFAQMVHGMVDAQLARLRE
                     AAA"
     gene            6085..>6554
                     /locus_tag="PLW13_12855"
     CDS             6085..>6554
                     /locus_tag="PLW13_12855"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY71315.1"
                     /translation="MKKALVTGITGQDGAYLAELLLSKGYEVHGIKRRASLLNTDRID
                     HLYQDPHVERRNLLLHYGDLTDSSALTRVIQTVQPDEIYNLGAMSHVAVSFEVPEYTA
                     NADALGALRILEAIRLLGLERKTRFYQASSSELYGLVQEVPQKETTPFYPRSPYA"
BASE COUNT         1061 a         2100 c         2233 g         1160 t
ORIGIN      
        1 tcctgaaccc ggaggcgaaa tgagcggcgt cctctgcttc acgagcatca attaccatta
       61 ccttgcgaag gcgcgtgtac tcgcgtcgag cctcaagcgc ttccatcccg actggcactt
      121 cgtgctatgt gtgaccgatg ccatgccgga tggcggcact ctggatcttg cggatgagcc
      181 cttcgatgaa ttgctgatca cccaggaggt tctgggcgac ctgggcccgg gctggttctt
      241 caaacacgat gtcgtcgagg tttgcacggc ggtgaagggc ccattgctgg aaaaattctg
      301 tgatcggggt ttcgacgcga tcgtttatct cgatcccgac attgcggtgt tcaatccgtt
      361 gcaaggcctc atcgaggcgc tggagcgctc ggacatcctg ttgaccccgc accagctgga
      421 gcccgatgac cagtactacc agatcgtcga caatgaagtg tgctcactga ggcacggaat
      481 tttcaacctc ggcttcgtgg cgataaagcc ttcggccgaa ggcaggcgat ttgcccggtg
      541 gtggagcgcg cggctcacgg atttctgcgt ttccgatgag cgcctcgggc tgttcgtcga
      601 ccagaagtgg tgcgatcacg tcccggtctt tttcgacgga gtgaagatcc tgcgagatcc
      661 cggctacaac gtggcgagct ggaacctcag caggcggaag atcagcatcg acagatcggg
      721 caggatcctc tgcaaccgca agcttcttta tttctatcat ttcaccaagc tggggctggt
      781 cggcgatgca atgacccagc gctatgccaa ggacaacatc gaggtctacg agctctggtc
      841 gtggtaccgg cgaaaggtag aggattttgc cttcgacgac ttgcccgatg gctggtgggc
      901 gctgggacaa ttctccaacg gcgcgaagat cacgagcgaa caacggcgat gctaccggga
      961 caggcccgat ctccagactt atttccctga tccatatctg gcagaaggaa attcgtttta
     1021 cgaatggtgc gggcgaaacg catgatcggc tcccacggcg ctgcacgcgt agcggtcggg
     1081 caacaatact ttcgattggt tggcggtgag ggttcatgaa cgggtgtgcc tggaaggagc
     1141 gggtttttgt tcttggcgac agcaggacgg gcacctcctc gttccatcag ttgtttttgc
     1201 ttgcgggcct gaaatccatt cacttttacg aagaggaagc cgggatgctg ccgcatgttg
     1261 cgggcaatca tgccgagaat tccaggcgcc ttcttcgcta catcgggaat tccggcattc
     1321 aatgcttctc ggactatccg actcgcctct actacaagga gctccatgct gcgtatccgg
     1381 aagcgctttt catactgacg aagcgggtga acgtgaaagt ctggtgtgaa agcatgttga
     1441 attacttcga tcacatcacc gatatcgacg agcttgcgtt ccactatctg aggataaatg
     1501 cggacatctc gagatatttc gcggcgtccg gcaatttctg cgagattgcg attgacgctc
     1561 ctggtttcgc cggggctttc tgccggatca agcgtcactt gcggatcgac ggcgatgtgg
     1621 agttcccgca tgtcaaccga tggcaggaca aggagtaagt cactgccgtt caccctgatt
     1681 tccgaattgc gctagactgg ccgactttcc gataattgga ccgggcagaa atccgcaaat
     1741 gttgcgcgac ccgaacatcg tggcggtgga cctcacgccg gtcctgccgg gaggtgaaaa
     1801 cggcggggcc aaggtcttcg tgctggagct gctgcgaggt ctcgcggcgc aggcaccgca
     1861 gatgcgcttc gtcctgctaa cgcgcgtgga aacccatgac gagctcgccg tcctggacgg
     1921 gccgaacgtc gagcgccgcc tggtgctggg cgccgcgcaa ccgacccccg cgcggcccgc
     1981 cctgtgggtt cgcgtggcgc accggctggc gcgttacctc cccgggcgta tccgtgcgct
     2041 ggcctcccgc gcgggttacc ggctgatgcg ccaccggcgc cgcgctgccg ccccgggcct
     2101 gctgcagggg atcggcgcca agttgctgtt ctgcccgttc acggctccca cctacgccga
     2161 gcccggggtg ccggtggtca gcgtggtgca cgacctgcag tacctgagct atccccagtt
     2221 cttcgaggcc gccgatatcg gtcatcgcca ccaggtgttc cgcgccgcgt gtaacgaggc
     2281 gacgctgctg gtggcgattt ccgactacgc gcgcgactcc gtgatccgcc agggtgcgat
     2341 cgcgccggat cgcgtgcgca ccatccatca tcgcctcggc aagcgcctgg gcagcgccgc
     2401 gggcgcggac gagtcggtgc tcgcgcgact ggggctggcg cgcggacgct acctggtcta
     2461 cccggccaat ttctggaagc acaagaacca cgagatgctg ctgacggcgt tcgggatcgc
     2521 ggcgcatggc ggattgccgc aggatatgcg gctcgtgtgc accggagcgc ccggtgcgcg
     2581 gcgcgatttc ctggcgcagg cagccgatgc gatggggctg ggctcgcgcg tcgtctttcc
     2641 cggctacgtt ccgaccgagg cacttgcggc actgctcggc aacagcgcgg gcatggtgtt
     2701 cccctcgctc tacgagggct tcgggatgcc cgtgatcgag gcgatggcgg ccggcgtcgc
     2761 ggtggcatgc agcaacgcga cctcgttgcc cgagatcgcc accggggcgg cgctcctgtt
     2821 cgatccgcgc attcccgggc agatcgcggc ggcgatgctc gaactggtca acgaccccgc
     2881 gcggcgcgcc gatctggttg ccgcggggct cgagcgcgcg gccggattca gcgatgccgg
     2941 gcggatggcg gacgaatacc gcgacgcgtt ccgccaggcg atggggcagg cgctgccacg
     3001 cccgggccgg ggaggcgcgg gcgcgtgaaa gtcagcgtca tcacgccatc gttcaaccag
     3061 ggccgcttca tcgagcgctc catccgcagc gtcgccacac agggcgtggc ggaactggag
     3121 cacgtcgtga tggacggcgg cagcaccgac gagacgcgcg ccgtgctcga acgctgcggc
     3181 gccacggtgc gctgggtttc ggaaaaggac cgcgggcaga ccgacgcgct gaacaagggc
     3241 atccgcgcca ccaacggcga gatcatcggc tggctgaatt cggacgacgt ctactacccc
     3301 ggcgcgatcg cgcgggtgct ggaggtgttc cgtacgcagc ccggggtcga cgtggtctac
     3361 ggcatggcgg accacatcga catcgacgac cgtgccttcg aggaatatcc caccgaagcg
     3421 tgggacctgg aacggctcaa ggagacctgc ttcatctgcc agccggcgct gttcctgcgc
     3481 cgccgcgtgg tcgagcagca cgggctgccc gacgaatcgc tgcactactg catggactac
     3541 gagtactggc tgcgcctggg caagggcggc gcgcgcttcc actacctcga ggagaagctg
     3601 gccgggtcgc ggctctacgg cgagaacaag acgctggggg cgcgggtggc ggtgcatcgc
     3661 gagatcaatg acatgatgcg gcggcatttc gggcgcgtgc ccgagcgctg gctgttcaat
     3721 tacgcgcatg cggtggtcga gccccgggtc gagcgcgcgc gcgcgccgcg gcgcttcgtg
     3781 ctgtgcctgg cgctgcactc gatcgccgcg gcgttgcgct ggaaccacgg catcggtccc
     3841 gcgatgcggc gcgtgctctg gcactggggg cgcgatgcat atcggctttg atatctcgca
     3901 gacgggcgcg ggcaaggcgg gatgcggcta ctacgccgac gccatgctgc aggcgctgct
     3961 caggctcgcg cccgcgcacc gcttctcgct ctatccgggt tttggcgatt tctacttcga
     4021 tgcgcgcatg ccgctgcgca atccgtaccc gggcgcgaac gccgaatacg gcccgcgcca
     4081 cctgacgcgc gagagcgcgg ccgagttctg gaatgcgccc gccctggccg atgcgctggg
     4141 ccggccggac atcgtgcacg ccaacaactt ctggtgcccg ccacggctcg agggctcgcg
     4201 gctggtgtat acgctctatg atctcgcgtt cctggtcgat ccgtcgtgga ccaccgaggc
     4261 caaccgcatc ggctgtttcg agggtgtgtt ccgttcttcc atcgccgcgg actgggtcgt
     4321 ggcgatctcg caagcctcgc gtggcgacta cctgcgcacc tttccgcact ttcctgccga
     4381 gcgggtacgc gtgatccacc cgtgctcgcg ctttttcgcc gccgacgacc tgccgggcga
     4441 gcgccatgag cgcctgcgcc acgtggcgcc gggacgcttc tggctgagcg tgggcaccat
     4501 cgagccgcgc aagaaccagc gccggctggc cgaggcattc gccgggtacc tcgcgggcgg
     4561 cggcgagccg atgccgctgg tgctggcggg aggggagggc tggcgcatgg agggctttcc
     4621 cctgttcctc gaggaactcg gcatcgcgga gcacgtgatc gtcaccggct acgtcacgga
     4681 cgccgagctg atctggctgt accgcaactg ctacgccaac ctctatccct cgttgttcga
     4741 gggtttcggg ctgccggtgc tggaaggcat gcagttcggg gcgccgacga tcgcctcgtg
     4801 cagcagttcc atccccgagg tcgcgggcga ggcggcggtg ctgctggcgc ccgaggatgc
     4861 ccgcgcgtgg acgcgggcca tgctggagct ggccgcccat cccgtgcagc gcgatgcgat
     4921 ggccgggcgc agccgggagc gggcggcgtg tttcgactgg aatcgcagcg cggggcagct
     4981 gctggcgctc tatgatgagg ccctgtcgca acctgccagg agccgcacgt gaaacgagcg
     5041 ctgatcaccg ggatcacggg acaggacgga tccttcctgg cggagttcct gctcgcgcgg
     5101 ggctacgcgg tgtacgggct ggcgcgccgc gagagctggt tccgcgccaa caacgccagc
     5161 cacctggccg ggcgcgtgga gatcctgttc ggcgacatga ccgagggcat cgacatcgcc
     5221 tccgcggtgc aggacgcgcg ccccgacgag atctacaacc tggcctcgca gtcgcgtccc
     5281 ggcgagtcgt gggcgcgcgc gcccgagacc ctgatgatca acggcatggc cgcgatccgg
     5341 ctgttcgaga ccgtgcgcca cgcctgtccg gcggcgaagg tctaccatgc ctcctcctcg
     5401 gagatgttcg ggcgcaccac cggggtggcc caggacgagg ccacgccgtt caacccgcag
     5461 aacccctacg ccgcggccaa ggtctacgcg caccagatgg cgcgcatcta ccgcgagagc
     5521 tacggcatgt tcatcgcgac cggcatcctg ttcaatcacg agagcgagcg ccggccgctg
     5581 cacttcgtga cccagaagat cgcctatggc gcggcctgcg cggcgctcgg tatcggcgac
     5641 tcgccggacc tgaacgagcg cgggcgcccc atcgtcgagc accgacacct cgcgctcggc
     5701 aacctcgagg tggcgcggga ctggggccat gcgcgcgatt tcgtgcgcgc gatgtggctg
     5761 atcctgcagc agggcacggc ggatgacttc gtggtgggga ccggtatagt gcacaccctg
     5821 cgtgatctct gcgatgcggc ctatgcccac gtcgggctgg actggcgcga gcacgtggtg
     5881 agcgatccgg cgctggtgcg gccgctggaa acggggcgca cggtggcgaa tcccgcgcgg
     5941 gcccgggcgc ggctcggctg ggagcccgag atcgggttcg cgcagatggt gcacggcatg
     6001 gtggatgcgc agctggcgcg gctgcgcgag gcggccgcct gaagcggcgg caaacattcg
     6061 ggttacaggc aggattttcg cggcatgaag aaagcgttgg tgacgggaat cacggggcag
     6121 gacggcgcct acctggccga gctgctgctg tccaagggct acgaggtgca cggcatcaag
     6181 cgccgcgcct cgctgctgaa caccgatcgc atcgaccacc tgtaccagga cccgcacgtc
     6241 gagcggcgca acctgctcct gcactacggg gacctgacgg attcgagcgc cctcacccgc
     6301 gtgatccaga ccgtgcagcc cgacgagatc tacaacctcg gcgcgatgag ccacgtggcg
     6361 gtcagcttcg aggtgccgga gtacacggcc aacgccgacg cgctgggggc gctgcgcatc
     6421 ctcgaggcga tccgcctgct cgggctggaa cgcaagaccc gcttctacca ggcgtccagc
     6481 tccgagctct acgggctggt gcaggaagtg ccgcagaagg agaccacgcc gttctacccg
     6541 cgcagcccct atgc
//
LOCUS       DAORUQ010000168         6498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_13835_length_6498_cov_6.107248, whole genome shotgun sequence.
ACCESSION   DAORUQ010000168 DAORUQ010000000
VERSION     DAORUQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13835_length_6498_cov_6.107248"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..862)
                     /locus_tag="PLW13_12860"
     CDS             complement(134..862)
                     /locus_tag="PLW13_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY71316.1"
                     /translation="MNPTQLRWSLSLVAAAVLHGVAFVWIRTSPTPAEPIALGSDVIE
                     LNFEVVAAVAEPTVPAHAAPTPPEPSPAPEPPPKPKPSPKPAPAPEPAPQPTPQPAEP
                     EPVTTTVSEPAPVEPAPASVPASAAPVAAVSTAKTRFTGKPARNVRSYFGQISAWIDA
                     NKDYPTEVKKKKQQGTVVVRFTIGREGQLLASTIKQSSGHVLLDRAALETLARAAPFP
                     PIPEFVGRETLSIAVPIDYTLITD"
     assembly_gap    1135..1234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1235..1734
                     /locus_tag="PLW13_12865"
     CDS             <1235..1734
                     /locus_tag="PLW13_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQY71317.1"
                     /translation="RLEGGSKAARRRLEGGEGAHEWAGYALAALAVLRIAWGCVGSRH
                     ARFCDFFPTPARLRRYLGRFPPDHEAWPGQNPLGALMVLVLWFMLLLTAVSGWMQEFD
                     AFWGEEWLQRLHELTADAVMIAVTIHVAAVLLMQRLTAAPLIRAMITGRREAWRGCPQ
                     RALRT"
     gene            1885..3252
                     /gene="chrA"
                     /locus_tag="PLW13_12870"
     CDS             1885..3252
                     /gene="chrA"
                     /locus_tag="PLW13_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQY71318.1"
                     /translation="MTDPAAARRDEAAIAPPSPVRLCEALRFWLKLGFIGFGGPAGQI
                     AIMHQELVERRRWISERRFLHALNYCMLLPGPEAQQLATYLGWLMHRTPGGILAGTLF
                     VLPSLFILIALSWVYIAFGHVALVAGLFYGIKPAVTAIVLHAAHRIGTRALKNRVLWG
                     IAAAAFVAIFALGLPFPLIVAGAALVGVVGGRFAPRYFRAGGGHGTPTASFGPALLDD
                     DTPPAAHVRFRWSGLLRVALVGALLWLLPMAALCAAFGWEHTLTQMGWFFTKAALLTF
                     GGAYAVLPYVYQGGVETYGWLSGPQMIDGLALGETTPGPLIMVVAFVGFVGGYVQALF
                     GPQAQFLAGALAACLVTWFTFLPSFLFILAGGPLVEATHNDLKFTAPLTAITAAVVGV
                     VLNLALFFGYHVLWPQGFGGAFDWAAALIALAAASALFRFGRGVMEVIAVCALLGLAL
                     RYAGA"
     gene            complement(3237..4553)
                     /locus_tag="PLW13_12875"
     CDS             complement(3237..4553)
                     /locus_tag="PLW13_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQY71319.1"
                     /translation="MEIAVLFALILLNGLFALSEVALLTARKARLQAGVEAGDKAAAA
                     ALRLGADPTRFLSTIQIGITSIGVLNGIVGEAALALPLAREMEELGARAPYSLYAATG
                     LVVLLITYITIVVGELVPKRIAQTHPEAIARLVARPLDWLAIATRPFVRLLSLSTHAL
                     LRVLRVPENGGATVTEEEIHAVLAEGTSAGVIELQEHAMVRNLFRLDDRRIGSLMVPR
                     SEVVCLDLDLAPEENLRRIGESAFARFPLVRGGLDNVLGVVSARGWLLHQLRGATPET
                     ESLALEPQLYVPESVTGMELLDKFRAGAAHLACVIDEYGEVQGIVTLHDLVEAITGEL
                     HPHDAGTSWAVQRGDGSWLLDGYIPVPELKDRLGLRAVPDEARGRYHTLSGMIMLLLG
                     RLPEVADGVDWEGWRFEIVDMDGKAIDKVLATRMPAGGDTRAQAPA"
     gene            4664..5176
                     /locus_tag="PLW13_12880"
     CDS             4664..5176
                     /locus_tag="PLW13_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQY71320.1"
                     /translation="MAADSPAPGWQVSRWFNTASPLDLAALRGRVVLLHAFQMLCPGC
                     VGHALPQAEQVHRLYSQQGVAVVGLHTVFEHHAAMTPVALEAFLHEYRITHPVGVDSA
                     VAGDPIPATMRAYGMQGTPTLVLLDRAGRIRLHQFGRVDDLPLGMLLGRLLAEGCDSN
                     PPMQSRGDAQ"
     gene            5173..6360
                     /locus_tag="PLW13_12885"
     CDS             5173..6360
                     /locus_tag="PLW13_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY71321.1"
                     /translation="MTERSKQGLATRSIHGQGLRDAHGSPHVPVYDTTTFAFGSTAEL
                     LDVIDGRKPGSLYTRYGMNPSILALENTLAGLEGAEMAWAFSSGMAAESALFLAHGRD
                     GIVCIGDAYGGTLELLAKQLPLLGITTHLLLGSEMDRLEAVLAGGARLVFLETPTNPT
                     LDVFDIRAIAHQAHAHGALLAVDNTFASPVNQRPLELGADLVTHSATKYLGGHSDLTA
                     GVLMGARDLLLPVWEWRKNLGSTIAPATASLLSRSLRTLVVRVRQHNASAQRIAEAMS
                     RHPAVRRVFYPGLAEFPGHALAKRQMYGFGGMLTIEVAGDGADATRVADRLELFALAP
                     SLGGVESLVTQPCTTTHHEIGAGERARRGISDAMLRLSVGLEDTEDLIADLEQALRAA
                     LGR"
BASE COUNT          901 a         2131 c         2250 g         1116 t
ORIGIN      
        1 ggccagtgcc acggccagtg tcagcgcgct gcgagctacc acggcgccgc gcggctgcgg
       61 gaggcgcagg ctcttcggtg ccttgatgcc ggtcgatggg tgcttcacgg ttgtctcctt
      121 gcggtggtgg ttgctagtcg gtgatcagcg tgtaatcgat cggcacggcg atgctgagag
      181 tctcgcggcc gacgaattcg ggaatggggg gaaagggcgc ggcgcgcgcc agggtttcga
      241 gagcggcccg gtccagcaac acgtggccgg agctctgctt gattgtgctg gcgagcagct
      301 gtccctcgcg cccgatggtg aacctgacca ccaccgtgcc ctgctgcttt ttcttcttca
      361 cctcggtcgg gtagtccttg ttggcatcta tccaggcgct gatctggccg aagtagctgc
      421 gcacgttgcg cgcaggcttg ccggtgaagc gggttttggc agtggagacg gcggccaccg
      481 gtgccgcgct ggcaggaacg cttgccggtg cgggctcgac cggcgcgggc tcgctcaccg
      541 ttgttgtcac cggctctggc tcggccggct gcggcgtggg ttgcggtgcg ggttccggcg
      601 ccggcgccgg ttttggactg ggcttgggct ttggaggtgg ttcgggtgcc ggtgacggtt
      661 cggggggcgt cggcgcggcg tgcgccggca cggtcggctc cgcgaccgcg gcgaccactt
      721 cgaaattgag ctcgatgacg tcgctgccca gtgcgatcgg ctcggcgggc gttgggctcg
      781 tcctgatcca cacgaacgcg accccgtgca ggacggcggc agccaccagt gacagcgacc
      841 accggagctg ggtcggattc atcgcgcgct tcttgggccg cggggccgga cgacccgcga
      901 caatgcgacg ccgcagccgg gcacagggcc ctgcggcggg cgtgagacgg tggaacgggg
      961 gcacacacga ggcatttcga gaattgtaag cttaataaga atgattcgta tttgtatata
     1021 ctgggtgtga cgatgtcaat accacatgga acgtcgcgca cggttcctgc tcgactactg
     1081 gcggctcgaa ggcggctcga aggcggctcg aaggcggctc gaaggcggcg agggnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcggc tcgaaggcgg ctcgaaggcg
     1261 gctcgaaggc ggctcgaagg cggcgagggt gcgcacgaat gggccgggta cgcgctcgcc
     1321 gcgctcgctg tgctgcgcat cgcctggggt tgcgtgggca gccggcatgc gcgcttttgc
     1381 gacttcttcc caacaccggc gagactccga cgctacctag gtcgcttccc gcctgaccat
     1441 gaggcctggc ccggccaaaa cccgctgggt gcgctgatgg tgctggtcct gtggttcatg
     1501 ctgctgctca cggccgtcag cggttggatg caggagttcg acgcgttctg gggcgaggag
     1561 tggttgcagc gcctgcacga actcaccgcc gatgccgtga tgatcgcggt gaccatccac
     1621 gtggcggccg tgctgctgat gcagcgactg acagccgcgc ccctgatccg cgcgatgatt
     1681 accggccggc gcgaagcctg gcgcggttgc ccgcagcgag cgctacgcac atgaccgcgc
     1741 agtagcgccg tccagccggg tcccggtgcg gtgagctgcg ttgtcatccg attgcggccg
     1801 tgatgttgca caatcacccg tgcggcacgc tcgcgtggca cagccggatg cgcacaccta
     1861 caagccccga ggaattttcg ccccatgacg gatcctgctg ccgcgcgtcg cgacgaggct
     1921 gcgatcgcgc caccatcccc cgtccgcctg tgcgaggcgc tccgtttctg gctgaaactc
     1981 ggctttatcg gcttcggcgg acccgccggg cagatcgcga tcatgcacca ggagctggtg
     2041 gagcgccggc gctggatctc ggagcgccga ttcctgcacg cgctcaacta ctgcatgctg
     2101 ctccccggcc ccgaggcgca gcagctggcc acctatctcg gctggctgat gcaccggacc
     2161 ccgggcggca ttctcgccgg gacgctgttc gtgttgccct cgctgttcat cctgatcgcg
     2221 ctgtcctggg tctacatcgc cttcggccac gtcgcgctgg tcgccggatt gttctacggc
     2281 atcaagcccg cggtgaccgc gatcgtgctg cacgccgcgc accgcatcgg cacgcgagcc
     2341 ctgaagaacc gcgtgctgtg ggggatcgcg gcggcggcct tcgtggcgat cttcgccctc
     2401 gggctgccgt tcccgctcat cgtggcgggc gcggcgctgg tgggcgtggt gggcgggcgg
     2461 ttcgcgccgc ggtatttccg cgccggtggc gggcacggta ccccgacggc ctctttcggt
     2521 ccggcgctgc tcgacgacga cacgccaccc gcggcgcacg tgcgctttcg ctggtcgggg
     2581 ctgctgcgcg tggcgctcgt cggggcgcta ctgtggctcc tgccgatggc cgccctgtgc
     2641 gccgcgttcg ggtgggagca cacgctgacg cagatgggct ggttcttcac caaggcggcg
     2701 ctgctcacct tcggcggcgc ctatgcggtg ctgccctacg tctatcaggg cggcgtggaa
     2761 acctacggct ggctcagcgg cccgcagatg atcgacggcc tggcgctggg cgagaccacg
     2821 ccggggccgc tgatcatggt ggtggccttc gtcggcttcg tcggtggcta tgtgcaggcg
     2881 ctgttcggcc cgcaggcaca gttccttgcc ggcgcgctgg ccgcctgcct ggtcacgtgg
     2941 ttcactttcc tgccgtcgtt cctgttcatc ctcgccggcg ggccgctggt ggaggctacg
     3001 cacaacgacc tgaagttcac cgcaccgctc accgcgatca ccgcggccgt ggtcggcgtg
     3061 gtgctcaacc tcgcgctgtt cttcggttac cacgtgctgt ggccgcaggg cttcggcggc
     3121 gccttcgact gggctgccgc actgatcgcg ctggccgcgg ccagcgcgct gttccgcttc
     3181 gggcgcggcg tgatggaagt gattgccgtg tgtgccttgc tggggctggc cttgcgctat
     3241 gccggggcct gagcgcgggt gtccccgccc gcaggcatgc gtgtggccag caccttgtca
     3301 atggccttgc cgtccatgtc gacgatctcg aagcgccagc cttcccagtc cacgccgtcg
     3361 gcgacttccg gcagccgccc gagcagcagc atgatcatcc cgctcagcgt gtggtagcgc
     3421 ccgcgcgcct cgtcgggtac ggcgcgcagc ccgagacggt ccttgagctc gggcaccggg
     3481 atgtagccgt cgagcagcca ggagccgtcg ccgcgctgca cggcccagga agtaccggca
     3541 tcgtgcggat gcaactcgcc ggtgatcgcc tcgaccaggt catgcagcgt gacgatgccc
     3601 tgcacctcgc cgtactcgtc gatcacacag gccaggtgcg cggcgccggc acggaacttg
     3661 tccagcagct ccatgccggt gacgctttcg gggacgtaga gctgcggttc gagcgccagg
     3721 ctctcggtct ccggggtcgc gccgcgcagc tggtgcaaca gccagccgcg cgcgctcacc
     3781 acgccgagca cattatcgag cccgccgcgt accagcggga aacgcgcgaa agccgattcg
     3841 cctatgcgcc gcaggttttc ttccggcgcc aggtcgagat cgaggcacac gacttccgaa
     3901 cgcggcacca tcagcgaacc gatccgccgg tcgtcgaggc ggaacaggtt gcgcaccatg
     3961 gcgtgctcct gcaactcgat cacgcccgcg ctggtgcctt cggcgagcac ggcgtggatt
     4021 tcctcctcgg tcaccgtcgc ccccccgttc tcggggacgc gcagcacgcg caacagcgcg
     4081 tgcgtcgaca gcgacagcag gcgcacgaac ggcctggtgg cgatggccag ccagtcgagc
     4141 ggccgcgcca ccagccgcgc gatcgcctcc gggtgcgtct gcgcgatccg cttgggcacg
     4201 agttcgccaa cgacgatggt gatgtaggtg atgagcagca ccaccagccc cgtggccgcg
     4261 tacaggctgt agggcgcgcg cgcacccagc tcctccatct cccgggccag tggcagggcc
     4321 agcgccgctt cgccgacgat gccgttcagc acgccgatcg atgtgatgcc gatctggatc
     4381 gtcgacagga aacgcgtggg gtcggcgccg aggcgcaggg ccgcggcggc ggctttgtcg
     4441 ccggcctcga ctcctgcctg cagcctggcc ttgcgcgcgg tgagcagggc gacttccgac
     4501 agcgcgaaca ggccgttcag cagtatcagc gcaaacagga ccgcgatttc catggcgtgg
     4561 gccggtgtgg gcggggcggg tgattgtaaa cgctgcggcc cgggctatcg tgacgatgta
     4621 cgatacgaac gtatgggtgg agcggtgtaa aggagggcgt gcgatggcgg cggacagtcc
     4681 agcacccggg tggcaagtga gccgctggtt caatacggca tcgccgctgg acctggccgc
     4741 gttgcgcggc cgcgtcgtgc tgctgcacgc cttccagatg ctgtgccccg gctgcgtcgg
     4801 tcacgcgctg ccgcaggccg agcaggtgca ccggctctac tcgcagcaag gggtcgccgt
     4861 cgtgggcttg cacacggtgt tcgagcatca cgccgcgatg acccccgtgg cgctcgaagc
     4921 cttcctgcac gaataccgca tcacgcaccc ggtgggcgtg gacagcgccg tcgctggcga
     4981 cccgattccg gcgaccatgc gcgcctacgg catgcagggc acgccgacgc ttgtcctgct
     5041 cgatcgcgcc ggacgcatcc gcctgcacca attcggccgt gtcgatgacc tgccgctggg
     5101 catgctgctg ggacggctgc tggcggaggg ttgcgactcg aatccaccca tgcaatcccg
     5161 gggagacgcg cagtgactga acgcagcaag caaggcctgg cgacgcgcag catccacggc
     5221 cagggtttgc gcgacgcgca tggcagtccg cacgttccgg tctacgacac gaccacgttc
     5281 gcgttcggtt cgacagcgga gctgctcgac gtgatcgacg ggcgcaagcc gggcagcctg
     5341 tatacccgct acggcatgaa cccgtcgata ctcgcgctgg aaaatacgct ggccggcctt
     5401 gaaggcgcgg aaatggcctg ggcgttcagc tccggcatgg cggcggaatc tgccctgttc
     5461 ctggcccacg gacgcgatgg catcgtctgc atcggcgatg cctacggcgg cacgctggaa
     5521 ctgctggcca agcagctgcc gttgctgggg atcacgactc atctgctgct cggcagcgag
     5581 atggaccgcc tcgaggccgt gctggcgggc ggtgcgcggt tggtgttcct cgagacgccg
     5641 accaatccga cgctggacgt gttcgacatc cgcgcgatcg cgcaccaggc gcatgcccac
     5701 ggcgcgctgc tggcggtcga caacacattc gcctcgccgg tgaaccagcg cccgctggaa
     5761 ctcggcgccg atctggtcac gcacagcgcg acgaagtatc tcggtggcca cagcgatctc
     5821 accgcgggcg tgctgatggg cgcgcgggac ctgctgctgc cggtgtggga atggcgcaag
     5881 aacctcgggt cgacgatcgc gcccgcaacc gcatccttgc tgtcgcgcag cctgcgcacc
     5941 ctggtcgtgc gcgtgcgcca gcacaatgcc tcggcgcaac ggatcgccga agccatgagc
     6001 cgccatcccg cggtgcgtcg tgtcttctat cccggtctcg cggagtttcc gggtcatgca
     6061 ctggcgaagc ggcagatgta cggcttcggg ggcatgctca ccatcgaggt ggcaggcgac
     6121 ggggcggacg ccacgcgcgt ggccgaccgg ctcgagctgt ttgcgctcgc acccagcctc
     6181 ggcggtgtcg aaagcctggt gacgcagccg tgcacgacca cccatcacga aatcggtgcc
     6241 ggggaacgcg cccggcgcgg catctcggac gcgatgctgc ggctgtccgt ggggctcgag
     6301 gacaccgagg accttatcgc cgacctggag caggcgcttc gcgccgcgct cggacggtag
     6361 tgacggattc gcgtttactt gatgcaaacg tcgttaatat ctgctttttt cgggttttga
     6421 tcgcgcttat cgtgccgcct ccatccggca acaggagtct ggccatggag aatcgctcgt
     6481 accgcaaccc gaacgtcg
//
LOCUS       DAORUQ010000169         6410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14154_length_6410_cov_4.691896, whole genome shotgun sequence.
ACCESSION   DAORUQ010000169 DAORUQ010000000
VERSION     DAORUQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14154_length_6410_cov_4.691896"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLW13_12890"
     CDS             <1..369
                     /locus_tag="PLW13_12890"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HQY71322.1"
                     /translation="RFPEAARWILMEFGLMVFIVGVGLQAGGQIVETFMDAGATLILA
                     AVLAVSLPLLLGYAFGRKVLHLHPVLLLGALTGAMTSAAAMSLVNQEAKSTMPALGYT
                     GTYAFANVILTVAGTLIMFV"
     gene            complement(383..1306)
                     /locus_tag="PLW13_12895"
     CDS             complement(383..1306)
                     /locus_tag="PLW13_12895"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HQY71323.1"
                     /translation="MSPGATRLHGPAWLSGALHLFILAGAAHSERPEAWPWALFAMAA
                     VSFFAWMANYRRYRQIHDLPTSRIASAAQGYVELFGRCESLPASALASPLSATACCWH
                     AWRIRRRNSRDQWETVDSGRSVAPFQLIDDTGHCLVMPEGAEVLSGHTKTWREGEREY
                     TEWLLLPYTPLYAIGAFATTAADTVTAREEHRAMADLLAEWKNRPAELHARFDLDSDG
                     QLDLREWELARLEARRELRRRRAAAPAARDLHTMQRPRDGRLFLLANELPERIGLRYR
                     WWARGHLMLFLVLGAAGLWLLRLPGGGSALP"
     gene            complement(1323..1898)
                     /locus_tag="PLW13_12900"
     CDS             complement(1323..1898)
                     /locus_tag="PLW13_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY71324.1"
                     /translation="MGAMMMIVPAVLLVAAAYAVMLYNGLVQLKHNVSRAWANIDVLL
                     KQRHDELPKLVETCKQYMKFEQETLTRVMEARARVATARESGSVGSLGQAEGGLRATL
                     GQLFAVAEAYPDLKTDQTFQQLQTRISALENAIADRREFYNESVNVNNVRIEQFPASL
                     LAGFFGFKPAELLEFAAAETADVDLGTLFRS"
     gene            complement(1936..3588)
                     /gene="panP"
                     /locus_tag="PLW13_12905"
     CDS             complement(1936..3588)
                     /gene="panP"
                     /locus_tag="PLW13_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative pyridoxal-dependent aspartate
                     1-decarboxylase"
                     /protein_id="HQY71325.1"
                     /translation="MSDAHKTAEATEENLRRIFTVPEAPDSTLARLEAEIARNLHGFL
                     NARIVATQRSLADIEADFRDARIPEQPIFVCEQADFLLEKVVAESVHTASPAFVGHMT
                     SALPYFMLPLAQIMIALNQNVVKTETSKAFTPLERQVLGMLHHLVYAREEPFYAQFLQ
                     DSRHALGAFCSGGTIANLTALWVARNNALPADGDFAGVGHEGMHRALRHHGYDDAAIL
                     VSRLGHYSLNKAADVLGIGRKSVHAIEVDACNRIDLRALEARCRQLRAGRSKIIALVG
                     IGGTTETGNIDPLASMAAIAREHGAHFHVDAAWGGPTLFSARWRHLLAGIELADSVTL
                     DAHKQLYVPMGAGMALFRDPAAVHAIEHHARYIIREGSRDLGSKTLEGSRPGMAMLVH
                     SGLRILGRRGYELLIDGGIDKARWFADEIAATPDFELTSAPELNILTYRYMPAAARQA
                     LAGADAGTVARINGLLNWITRDIQRLQRDAGKSFVSRTQLGLARYGDEAIDVFRVVLA
                     NPLTDRQVLREVLSEQRGIAASAALAAQRAQLDAALAPAVAV"
     gene            3805..4812
                     /locus_tag="PLW13_12910"
     CDS             3805..4812
                     /locus_tag="PLW13_12910"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HQY71326.1"
                     /translation="MLRRTRKGFPVGRVFVLVSGLAWGAAVAAGSLRDCAGVAEAAAR
                     LACFDRLAAEAAATRIDEVPVAAAKPAATGGALQSRFRKESNLLENPFGLTAHKPNYM
                     LPVTYNSKIHEEPWTSIYPDANMDDIEAKFQISFKAQLWDISERFGLWAAYTQESWWQ
                     VYNDEESAPFRETNYQPEIYLSYDTRAELFGFRVAQLNLGFNHQSNGRSALLSRSWDR
                     IVAGALLERDNLAISPRLWYRLPEDKEDDDNPDTEDYYGYGDLRIAYLWHDQTFSALA
                     RNNLQSDDNRGALQLDWSFPLNRRFKGYVQYFYGYGESLIDYDVKTNRLGVGVMLTDW
                     L"
     gene            5023..5853
                     /locus_tag="PLW13_12915"
     CDS             5023..5853
                     /locus_tag="PLW13_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71327.1"
                     /translation="MANTFSGQLVLVTGAGSGIGLEIALAFARAGARLVITDVNTSAL
                     EVAEAAVTALGAPCMAARCDVTSEQEVLALAERVKAQAGVPEILVNNAGIGYFGQFLD
                     TPPAAWRRVLDVNVMGVVHGIRAFLPAIRQAGGERWLVNVASTAGFAPAPSMSAYAAS
                     KHAVVGLSEVLAMELDGSTVRVMVVCPGVINTPIVAGNAQLAPEIPRSQIERLQAYYV
                     AHGCPPREVADALLRALARGEQLLFAGPLARSASILMRISRRLARRVTLRKAHDMGYL
                     "
     gene            complement(5915..>6410)
                     /locus_tag="PLW13_12920"
     CDS             complement(5915..>6410)
                     /locus_tag="PLW13_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY71328.1"
                     /translation="ESVFVNVKNRSKTITAELEVPQGGANGTILAQGGRFGGWSLYVK
                     DGVPAYNYNFLGLEQTSIAASKPLAPGKATLKFDFAYDGGGPGKGGVGTLYVNGEKVA
                     EGRIEQTQGGIFSADETADVGIDLGTPVVEAVGAEAGSKFNGHIPKLSVEVRAARIAA
                     AREP"
BASE COUNT          966 a         2171 c         2221 g         1052 t
ORIGIN      
        1 agatttccgg aggcggcacg gtggatactg atggaattcg gcttgatggt cttcatcgtc
       61 ggtgtcggct tgcaggccgg cggccagatc gtggagacct tcatggacgc gggggctacc
      121 ctgatcctgg ccgcggtact ggccgtctcg ctgcctctgc tgctcggcta cgcattcggt
      181 cgcaaggtgc tgcatctgca tcccgtgctg ctcctgggag cgctgaccgg tgccatgacc
      241 agcgcggccg cgatgagcct ggtcaaccag gaggcgaaga gcacgatgcc ggcgctcggt
      301 tatacgggaa cctatgcgtt cgccaatgtc atcctgacgg tggccggcac cctgatcatg
      361 ttcgtgtgag gcgggtcgcg cctcacggca gcgcgctgcc gccgcccggc aggcgcagca
      421 accacagccc ggcagcgccc aggacaagga acagcatgag gtgcccccgg gcccaccacc
      481 ggtagcgcag cccgatgcgt tcgggcagtt cgttggcgag caggaacagc cgcccgtcgc
      541 gcggacgctg catcgtgtgc agatcccggg ctgcgggggc agcggcgcgg cgccggcgca
      601 gctcgcggcg cgcctcgagc cgcgcgagct cccattcgcg caggtcgagc tgcccgtcgc
      661 tgtccaggtc gaagcgcgcg tgcagttctg cgggcctgtt cttccactcg gccagcagat
      721 ccgccatcgc gcggtgttcc tcgcgcgctg tcacggtgtc ggctgcggtg gtggcgaagg
      781 caccgatggc gtagagcggg gtgtacggga gcagcagcca ttcggtgtat tcgcgctcgc
      841 cctcgcgcca ggtcttcgtg tgtccggaca gcacttcggc gccctcgggc atcacgagac
      901 agtggcccgt gtcgtcgatg agctggaatg gcgcgacgct gcggcccgaa tccacggtct
      961 cccactggtc gcggctgttc ctgcggcgta tgcgccaggc atgccagcag caggcggtgg
     1021 cgctcagcgg gctcgcgagc gccgaggcgg gcagcgactc gcagcggccg aacagttcga
     1081 cgtagccctg cgcggccgag gcgatgcgcg aggtcggcag gtcgtggatc tggcggtagc
     1141 ggcggtagtt cgccatccac gcgaaaaagc tcaccgcggc catcgcgaac agcgcccagg
     1201 gccaggcctc ggggcgctcg gagtgcgcgg cgccggcgag gatgaacaga tgcagcgcgc
     1261 ccgagagcca tgcaggcccg tgcagccgag ttgccccggg gctcatgcgc gcgcgtccgg
     1321 gctcaggaac ggaacagcgt gccgaggtcg acgtcggcgg tttcggcggc cgcgaactcg
     1381 agcagctccg ccggcttgaa gccgaagaag cccgcgagca gcgaggccgg gaactgctcg
     1441 atgcgcacgt tgttcacgtt gaccgactcg ttgtagaact cgcgccggtc ggcgatggcg
     1501 ttctccagcg ccgagatgcg cgtctgcagc tgctggaagg tctggtcggt cttcaggtcg
     1561 gggtaggcct cggccaccgc gaagagctgg ccgagcgtgg cgcgcagccc gccctcggcc
     1621 tgccccagcg aacccacgct gccggattcg cgcgccgtgg ccacccgcgc ccgcgcctcc
     1681 atcacgcgcg tgagcgtctc ctgctcgaac ttcatgtact gcttgcaggt ctcgaccagc
     1741 ttcggcagct cgtcgtggcg ctgcttcagc agcacatcga tgttggccca cgccctggac
     1801 acgttgtgct tgagctgcac caggccgttg tagagcatca cggcgtaagc cgcggccacc
     1861 agcagcactg ccggcacgat catcatcatt gcacccatgg ttgcctccgc aagcgacgtt
     1921 catccctgtc cgattctaga ccgccacggc tggagccagc gcggcgtcga gctgcgcgcg
     1981 ctgcgccgcc agcgccgccg aggccgcgat cccgcgctgc tcactcagca cctcgcgcag
     2041 cacctgccga tcggtgagcg ggttggcgag cacgacgcgg aacacgtcga tcgcctcgtc
     2101 gccgtagcgc gcgagcccga gctgcgtgcg cgagacgaag gacttgcccg cgtcgcgctg
     2161 cagacgctgg atgtcgcgcg tgatccagtt cagcagcccg ttgattcgcg ctaccgtgcc
     2221 cgcatccgcg cctgcgagcg cctggcgcgc ggcggcgggc atgtaacggt aggtgaggat
     2281 gttcagctca ggcgcgctgg tgagctcgaa gtccggcgtc gcggcgattt catcggcgaa
     2341 ccagcgtgcc ttgtcgatgc cgccgtcgat cagcagctcg taaccgcggc gcccgaggat
     2401 gcgcaggccc gagtgcacca gcatcgccat gcccgggcgc gagccctcca gggtcttgct
     2461 gccgagatcg cgcgaaccct cgcggatgat gtagcgcgcg tggtgctcga tggcgtgcac
     2521 ggcggcgggg tcgcgaaaca gtgccatgcc cgcgcccatc ggcacgtaga gctgcttgtg
     2581 cgcgtccagc gtgacggagt ccgcgagctc gatgccggcg agcaggtggc gccagcgcgc
     2641 ggagaacagc gtcggcccgc cccaggccgc gtcgacgtga aaatgcgcgc cgtgctcgcg
     2701 cgcgatcgcg gccatcgagg cgagcggatc gatattgccg gtttcggtgg tgccgccgat
     2761 gccgaccagc gcgatgatct tcgagcggcc cgcgcgcagc tggcggcagc gcgcctcgag
     2821 cgcgcgcaga tcgatgcggt tgcaggcatc gacctcgatc gcgtgcaccg actttcgccc
     2881 gatgccgagc acgtcggccg ccttgttcag cgagtaatgg cccaggcgcg ataccaggat
     2941 cgcggcatcg tcgtagccgt ggtggcgcag cgcgcggtgc atgccctcgt ggcccactcc
     3001 cgcgaagtcg ccgtccgcgg gcaaggcatt gttgcgcgcc acccacagcg cggtgagatt
     3061 ggcgatggtg ccgccggagc agaaggcgcc gagcgcatgg cggctgtcct gcaggaactg
     3121 cgcgtagaac ggctcctcgc gcgcgtagac caggtgatgc agcatgccca gcacctggcg
     3181 ctccagcggg gtgaaggcct tggaggtctc ggtcttcacg acgttctggt tcagcgcgat
     3241 catgatctgc gccagcggca gcatgaagta gggcagcgcc gaggtcatgt gaccgacgaa
     3301 ggcgggcgat gccgtgtgca ccgactcggc gacaaccttt tcgagcagga agtctgcctg
     3361 ctcgcagacg aaaatcggct gttcgggaat gcgtgcgtcg cgaaaatccg cttcgatgtc
     3421 cgccaggctg cgctgcgtgg cgacgatgcg cgcgttcagg aagccgtgca ggttgcgcgc
     3481 gatctcggcc tcgaggcggg ccagcgtgga gtccggcgcc tcggggacgg tgaagatcct
     3541 ccgcaggttc tcctcggtcg cttccgctgt cttgtgcgca tcgctcatgg cagccctgat
     3601 cctcccgccg ggcgcgaccg gtctggccgc cacagcgccc gatcatggcg tgcgcgcctg
     3661 tgcgatgcaa cgttcggcag gctggcaacg gcacggctgt tcggccctcg cgacgacacg
     3721 gcgacaattt cacactgacg cctgcgttgc cgcgaacgcg gcgccgttat actcgcaagt
     3781 tcaaatgtca acggttccag tccaatgcta cggcgtacgc gcaagggttt tcctgtcggc
     3841 agggtgttcg tgttggtgtc gggtcttgcc tggggcgcgg cggttgcggc ggggagcctg
     3901 cgcgattgtg cgggcgttgc cgaggccgcg gcgcgtctgg cgtgcttcga ccggctggcg
     3961 gccgaggcgg cagccacgcg catcgacgag gtgccggtcg ccgcggcgaa gccggccgcc
     4021 acgggtggcg cgctgcaatc gcgctttcgc aaggagtcga acctgctgga gaatcccttc
     4081 ggcctgaccg cgcacaagcc gaactacatg ctcccggtca cctacaacag caagatccac
     4141 gaagagccct ggacgtccat ctaccccgac gcgaacatgg acgatatcga ggcgaaattc
     4201 cagatcagct tcaaggcgca gctgtgggac atcagtgaac gtttcggcct ttgggcggcc
     4261 tacacgcagg aaagctggtg gcaggtctac aacgacgagg agtccgcgcc gttccgcgaa
     4321 accaactatc agcccgagat ctacctgtcc tacgacaccc gggccgagtt gttcgggttc
     4381 cgcgtggcgc agctcaacct cggattcaac caccagtcga acggccgcag cgcactgctc
     4441 tcgcgcagct gggaccgcat cgtcgccggg gccctgctgg agcgtgacaa cctcgcgatc
     4501 tcgccgcgcc tctggtaccg gctgcccgag gacaaggaag acgacgacaa ccccgacacc
     4561 gaggactact acggttacgg ggatctgcgc atcgcgtatc tctggcatga ccagaccttc
     4621 tcggcgctgg cgcgcaacaa tctgcagagc gacgacaacc gcggcgcgct gcagctcgac
     4681 tggagctttc cgctcaaccg ccgcttcaag ggctatgtgc agtacttcta cggctacggc
     4741 gagagcctga tcgactacga cgtgaagacc aaccgtctcg gcgtcggcgt gatgctgacc
     4801 gactggctgt aacgcccctg accggccgca tctggcgcag gccggcgaac cgtagctgcc
     4861 ccggccgctg tatggatcct gtctcgcttc gcgggtgatg attcaacggc cagggccacc
     4921 gacgccaggg acaggcgccc ggagtgcccg agcatgtagt ctggcccgat cgtacaggcc
     4981 ggcagtgcgt gtgcctgatc cctggttgcg gagacttcag cgatggccaa cacgttcagc
     5041 ggccaactcg tgctcgtgac gggcgccggc tccggcatcg gcctggagat cgcgctcgcc
     5101 ttcgcgcgcg caggtgcgcg gctggtcatc accgacgtga acacgagcgc gctggaagtc
     5161 gccgaggcgg ccgtgaccgc gctcggcgct ccctgcatgg cggcgcgctg cgacgtcacc
     5221 agcgagcagg aggtgcttgc cctcgcggaa cgggtgaagg cgcaggcggg ggtcccggag
     5281 atactcgtca acaatgcggg catcggctat ttcggccagt tcctcgatac gccccccgcg
     5341 gcgtggcgtc gcgtgctcga cgtgaacgtg atgggcgtgg tgcacggcat ccgcgccttc
     5401 ctgcccgcga tccgccaagc cggcggcgag cgctggctcg tcaacgtcgc ctccacggca
     5461 ggcttcgcgc ccgcgccgtc gatgagcgcc tatgccgcgt ccaagcacgc cgtggtcggg
     5521 ctgtccgaag tgctcgcgat ggagctcgac ggcagcacgg tgcgcgtgat ggtggtctgt
     5581 cccggcgtca tcaatacccc gatagtggcg ggcaacgcgc agctggcgcc cgagatcccg
     5641 cgcagccaga tcgagcgcct gcaggcgtat tacgtcgcgc acggctgccc gccgcgcgaa
     5701 gtcgccgacg cgctgctgcg ggcgctggca cgcggcgagc agctgctgtt cgccgggccg
     5761 ctggcgcgct cggcgtcgat cctgatgcgg atctcgcggc ggctggcgcg gcgcgtgacg
     5821 ctgcgcaagg cgcacgatat gggctacctg tgatgcccgc agcgttcccg cagttgcacg
     5881 cccggggata aggatctcgg aatcgtcctg tcgttcatgg ctcccgggca gctgcgatcc
     5941 gggcggcgcg aacttccacg ctcagcttcg gtatgtgccc gttgaacttc gacccggcct
     6001 cggcgccgac ggcctcgacc acgggcgtgc cgaggtcgat gccgacgtcg gccgtctcgt
     6061 ccgcggagaa gatcccgccc tgggtttgct cgatccgtcc ctcggcgacc ttctcgccgt
     6121 tgacgtagag cgtgcccacg ccgcccttgc cgggcccgcc gccgtcgtag gcaaagtcga
     6181 atttcagcgt cgccttgccc ggcgccagcg gcttgctcgc cgcgatgctg gtctgctcca
     6241 ggcccaggaa gttgtagttg taggccggca cgccgtcctt gacgtacagc gaccagccgc
     6301 cgaagcggcc accctgcgcc aggatggtgc cgttggcgcc gccctgcggc acttccagtt
     6361 ccgccgtgat ggtcttggaa cggttcttca cgttgacgaa cacgctctcc
//
LOCUS       DAORUQ010000170         6403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14179_length_6403_cov_5.498425, whole genome shotgun sequence.
ACCESSION   DAORUQ010000170 DAORUQ010000000
VERSION     DAORUQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14179_length_6403_cov_5.498425"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLW13_12925"
     CDS             <1..247
                     /locus_tag="PLW13_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71329.1"
                     /translation="ICGASQCRAVGEKGEAEALAKVHRLGIVCPVLRAVTIANGRLAC
                     LRRYAARPGLLRLRRESAREAARCHPRPTLAAGHPCG"
     gene            298..1788
                     /locus_tag="PLW13_12930"
     CDS             298..1788
                     /locus_tag="PLW13_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY71330.1"
                     /translation="MRRMIAAGERLFRPHRMRLLMATALVPAVVLVACSGQPRQQQAE
                     AKTPDELYAAMEQSLTRYRDGVQALREGDNDTARQLLTSAATELATGGDACVSTPGCE
                     PQRFMSAYGNLLSLRSAVLTNAAEGFAQIEPRPQVEAAMLSSDVPEAQRSVNLLHGHD
                     LAKIIKINGPVKAALHDWLTWMRPNLIDAYENYEYMRHLMWPAYEEAGLPEALLFGIM
                     AKESGGKVHAVSQSGAAGPLQFMYHTGLRFGLNNQNGFDTRYDPAAAARANVAYLNEQ
                     FRHLNNNLEMALAAYNGGEGRMRRLAQSSRSKNYWSGEIQGKLPRETQEYVPYVLAAA
                     WLFLHPEDYGLEFPRVDASPGQVQLVKAMTLSELTVCLGQAGSRDGWFRTLRNLNPRV
                     DPNKRLPAGSSVAMPAPLAEVYRSQCVDGRMAELAMLLQDARQPGGVANGGGKRYVVK
                     RGDTLASIARKQGCDSPQALARANRIAAPRYTIKPAQELTLLGCNG"
     gene            complement(1808..2473)
                     /locus_tag="PLW13_12935"
     CDS             complement(1808..2473)
                     /locus_tag="PLW13_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQY71331.1"
                     /translation="MHAHAVTNPGAALLACMQRIAAAGLSQGSSGNASVRSAKGFWIT
                     PTGMAPQRMHADDAVEIDPGGRVLAGTLRPSSEWPMHAAIYRARPDAQAIVHCHSRHA
                     TALACCGRDIPAFHYMVAVAGGDSIRCSPYALFGSQALAEQAVAALRERRACLLGNHG
                     QIAIGDSLESALALAIEVEELAAQYGAALAIGEPRLLDGAQMREVIGRFAGYGQPQRR
                     DEP"
     gene            2592..5033
                     /locus_tag="PLW13_12940"
     CDS             2592..5033
                     /locus_tag="PLW13_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71332.1"
                     /translation="MATMVRPLAAFVRAAGALCAGAAVLGTVAPVALAQPPVSRVELE
                     EIVVTAQKREQESLDVPVAVGTFTSRDILNTGALTLQQIDDFIPGFEADGETFTQQSY
                     NVRGISSPNISTGGDPSVATFYDEVYLPRAATTMAFADMERVEVLMGPQGTLFGRNAA
                     VGVINMVPRAPADEFEAFVNVRGGNYNLLRVEGMVNVPVSDTFALRANVLSNERDGIA
                     RNVGPSSAQAADRDNQAARIAAAWDATDATRAYLAYDWDRFDQSPTMAIGISPYAYGT
                     DPFAGRYENDVREAEETRDMYGVTGKLFHDFSEAWSGKLILSYREWETTNREDEDGTA
                     DATRYFDTNNREDSDIFYSEMQFNFANDRIDAVFGANYSRENTFQSTDANALGDSITR
                     LVTNQLNSDLGLGLDHLWNPEEFASALNLFGIPVTGAEVAATGDFWYDTVAGALNEPM
                     IFGPSFRGVEWTEAIQNEGDFTNWGVYGDVAYHLSERLDVIGGLRYSEDEKEFSWLFP
                     PTSFAALRPGVSNQIFMLDPQYASAYTTPLESSDSWSQVTGRAVVQYFLSEDFMGYLS
                     YSTGYKSGGFDSLTISSAAEPLEPEESEMVEIGLKGDLLEGRLRAQVALFNLQVDGRQ
                     TTVDSRQPGTPNAIPTVINIDNENEGLELTLDWMVTDSLKLGGLTTVRNEDSTSGTFY
                     NSDAELTDMNNSGDTATTYTLRLDWSLEVPGGELLVHADYVYEENTIDENAPNFFEEL
                     RDLPRYFDDSEMLNARLAWISGDGHYEIALWGKNLLDKELIQGVRTITRSSFGTTFVG
                     IEDPLTWGLEGRYTW"
     gene            5039..>6403
                     /gene="aceK"
                     /locus_tag="PLW13_12945"
     CDS             5039..>6403
                     /gene="aceK"
                     /locus_tag="PLW13_12945"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HQY71333.1"
                     /translation="MNQAQSSTLSPAARRVARTILNGFESYFAEYQNITLGAKRRFET
                     ANWQGVHEASTERIDLYKSKISRVVRLVREVTSQDLSRLELWHEARSAYAQLVSSHSN
                     YEIAETFFNSIYCRIHDHSPIHDRYIFVKPSRALEDKPLPDYSIYVRYEMAGGLVDLV
                     SRILDDFAFTVPWEDKRRDVMSIVNLLQDQLLPGLDCPLEGLRVEMLESVFYRNKAAY
                     LVGRVSHAGGVIPVILPCLNNEKGGIYVDTVIHDQDGASIIFSFTRSYFMVDAPIPSR
                     YVRFLLSLMPQKGVAELYNSIGFNKHGKTEFYRDMIRHMKASEDLCVIAPGIKGMVMT
                     VFTLPSYGVVFKVIKDRFDPPKQVTEEIVREKYRFVSRADRAGRMADTQEYKNFIFYR
                     NRFSQELIDELQRVAPSKLWMDEKWIIIRHLYVERRMVPLNLYLRDAGEAEVYDAMDE
                     YGNAI"
BASE COUNT         1141 a         2114 c         2144 g         1004 t
ORIGIN      
        1 gatctgcggc gccagccagt gccgggcggt tggtgaaaag ggtgaggcgg aagcgctcgc
       61 caaggttcat cggctcggta tcgtgtgccc ggtgctgagg gcggtgacaa tagcaaacgg
      121 ccggctcgcc tgtctccggc gctacgctgc gcggcccggc ctgctccggc tgcgtcggga
      181 atccgcgcgt gaggccgcgc gttgccatcc gcgtccgacg cttgccgcag ggcacccctg
      241 cggctaacct tcgcggcagg acaacaggca acgctggctc aacgaaaggt atacaggatg
      301 cgcagaatga tcgccgccgg cgagcggctg ttccggccgc accgcatgcg cttgctgatg
      361 gcgacggcgc tggtgccggc cgtggtgttg gtcgcctgtt ccggccagcc gcgccagcag
      421 caggccgagg cgaagactcc cgacgagctg tatgcggcca tggagcagtc gctgacgcgc
      481 tatcgcgacg gcgtgcaggc actgcgcgaa ggcgataacg acaccgcgcg gcaactcctc
      541 accagcgcgg cgaccgagct ggccaccggt ggcgacgcct gcgtcagcac gccgggctgc
      601 gagccgcagc gcttcatgtc ggcctacggc aacctgctgt ccctgcgcag cgccgtgctg
      661 accaacgcgg cggagggttt cgcgcaaatc gagccgcgac cgcaggtcga ggccgcgatg
      721 ctctccagcg atgtgcccga ggcgcagcgc tcggtgaacc tgctgcatgg ccatgatctg
      781 gcgaagatca tcaagatcaa cggcccggtg aaggccgcgc tgcacgactg gctgacctgg
      841 atgcggccca acctcatcga cgcctacgag aactatgaat acatgcgtca cctgatgtgg
      901 ccggcctacg aagaggccgg cctgccggag gcgctgctgt tcgggatcat ggccaaggag
      961 tccggcggca aggtgcacgc ggtgtcgcaa tccggtgccg cgggtccgtt gcagttcatg
     1021 taccacacgg ggctgcgttt cgggctgaac aaccagaacg gcttcgacac gcgctacgat
     1081 ccggcggctg cggcgcgcgc caacgtggcg tacctgaacg agcagttccg ccatctcaat
     1141 aacaatctcg aaatggcgct cgcggcctac aacggcgggg aaggccggat gcggcgcctg
     1201 gcgcagagct cgcgcagcaa gaactactgg tccggcgaga tccagggaaa gctcccgcgc
     1261 gagacgcagg agtacgtgcc ctacgtgctg gcggcggcgt ggttgttcct gcacccggag
     1321 gactacgggc tggagttccc gcgggtcgat gcctcgccgg gccaggtgca gctggtgaag
     1381 gcgatgacgc tgagcgagct caccgtctgc cttggccagg ccggcagccg cgacggctgg
     1441 ttccgcaccc tgcgcaacct caatccgcgc gtcgatccca acaagcgcct gcccgcaggc
     1501 tccagcgtgg cgatgccggc cccgctggcc gaggtctacc gcagccagtg cgtcgacggc
     1561 cgcatggcgg agctcgccat gttgttgcag gacgcgcgcc agcccggcgg cgtggccaac
     1621 ggtggcggca agcgctacgt ggtgaagcgc ggcgatacgc tggcgagcat cgcgcgcaag
     1681 cagggctgcg acagcccgca ggcgctggcg cgcgccaacc ggatcgccgc gccgcgctac
     1741 acgatcaagc cggcgcagga gctcacgctg ctgggctgca acggctgaag cccgctccct
     1801 gcgcgggtca gggttcgtcg cgccgttgcg gctgcccgta accggcgaat cgtccgatca
     1861 cctcgcgcat ctgcgcgccg tcgagcaggc gcggctcgcc gatcgcgagc gctgcgccat
     1921 actgcgccgc gagctcctcc acctcgatcg ccagcgccag cgccgattca agcgagtcgc
     1981 cgatagcgat ctggccgtgg ttgccgagca ggcaggcgcg acgttcgcgc agggccgcca
     2041 cggcttgttc ggccagtgcc tgcgagccga acagcgcata gggcgaacag cggatcgagt
     2101 cgccgccggc cacggccacc atgtagtgga acgccgggat gtcgcgcccg cagcaggcga
     2161 gcgcggtggc gtggcgcgag tggcaatgca cgatcgcctg cgcgtcgggg cgcgcgcggt
     2221 agatcgccgc atgcatcggc cattcgctgg acgggcgcag cgtcccggcg agcacgcggc
     2281 cccccggatc gatctcgacc gcatcgtcgg cgtgcatgcg ttgcggcgcc attcccgtcg
     2341 gcgtgatcca gaaacccttc gcactgcgaa cggaagcgtt gcccgagctg ccctgcgaca
     2401 gcccggccgc cgcgatgcgc tgcatgcatg ccaggagcgc ggcgcccggg ttggtgactg
     2461 cgtgtgcgtg catcggatgc cctgctgacc gctggatggg tggatgctaa catccccggg
     2521 ccggcttcat cacgacgcga gcggcgccgg atccggcaca caacaacaac agcgattcgc
     2581 aaggggtatg catggcaacc atggtccggc cgctggccgc tttcgttcgc gccgcaggcg
     2641 cgctttgcgc cggcgcagcc gtgctgggca cggtggcgcc ggtggcgctg gcgcaaccgc
     2701 cggtctcgcg cgtggagctc gaggagatcg tcgtgacggc acagaagcgc gagcaggaat
     2761 cgctggatgt gccggtggcc gtgggtacct tcacgtccag ggacatcctg aacaccggcg
     2821 cgctgaccct gcagcagatc gacgatttca tcccgggttt cgaggctgac ggcgagacct
     2881 tcacgcagca gagctacaac gtgcgtggca tctcgagccc gaacatctcc accggaggcg
     2941 atccctcggt cgcaaccttc tacgacgagg tctacctgcc gcgtgccgcc accacgatgg
     3001 ctttcgccga catggagcgc gtcgaggtgc tgatggggcc gcagggcacg ctgttcgggc
     3061 gcaatgccgc ggtcggcgtg atcaacatgg tgccgcgcgc gccggcggat gaattcgagg
     3121 ccttcgtgaa cgtgcgcggc ggcaattaca acctgctgcg cgtcgagggc atggtgaacg
     3181 tgccggtgag cgatacgttt gccctgcgcg ccaacgtgct ctcgaacgag cgcgacggca
     3241 ttgcccgcaa cgtgggaccc tcgtccgcgc aggcggccga tcgcgacaac caggcggcac
     3301 gcatcgcggc cgcctgggac gcgacggacg cgacccgcgc ctacctggcc tacgactggg
     3361 accgcttcga ccagtcgccg acgatggcca tcggcatcag tccctacgcc tatggcacgg
     3421 atccgttcgc gggcaggtac gagaacgacg tgcgcgaggc cgaggagacc cgcgacatgt
     3481 acggcgtgac gggcaagttg ttccacgatt tcagcgaggc gtggagcggc aagctgatcc
     3541 tgagctaccg cgagtgggaa acgacgaacc gcgaggacga ggacggtacc gcggatgcga
     3601 cccgctactt cgacaccaac aaccgcgagg attccgacat cttctactcg gagatgcagt
     3661 tcaatttcgc caacgatcgc atcgacgcgg tgttcggcgc gaactacagc cgcgagaaca
     3721 ccttccagag caccgatgcg aatgcgctgg gcgattcgat cacgcgcctg gtgacgaacc
     3781 agctgaacag cgacctcgga ctgggcctgg atcacctgtg gaaccccgag gagttcgcct
     3841 ccgcgctgaa cctgttcggc attccggtca ccggcgccga ggtcgcggcg accggcgact
     3901 tctggtacga caccgtcgcc ggcgcgctga acgagccgat gatcttcggc ccctccttcc
     3961 gcggcgtgga atggaccgag gcgatccaga acgaggggga cttcaccaac tggggcgtct
     4021 acggtgacgt cgcctaccac ctgagcgagc ggctcgacgt gatcggaggt ttgcgctata
     4081 gcgaggacga gaaggagttc tcgtggctgt ttccgccgac cagcttcgcg gcgctgcgcc
     4141 ccggtgtgag caaccagatc ttcatgctcg atccgcagta tgcctcggca tacaccacgc
     4201 cgctcgagag cagtgacagc tggagccagg tcacggggcg cgcggtcgtg cagtacttcc
     4261 tcagcgagga tttcatgggc tacctgagct actcgaccgg ctacaagtcg ggcggcttcg
     4321 attcgctgac gatttcgagc gcagcggagc cgctggaacc cgaggaatcg gagatggtcg
     4381 agatcgggct gaagggcgac ctgctcgagg gccgcttgcg cgcgcaggtt gcgctgttca
     4441 acctgcaggt cgacggacgc cagaccacgg tcgattcgcg ccagcccggc acgcccaacg
     4501 cgatacccac ggtgatcaac atcgacaacg agaacgaggg cctcgagctc acgctcgact
     4561 ggatggtcac ggactcgctg aagctcggcg gcctgacgac cgtgcgcaac gaggattcga
     4621 cctcgggcac gttctacaac tccgatgccg agctcaccga catgaacaac tcgggcgaca
     4681 ccgcgacgac ctacaccctg aggctcgact ggtccctcga ggtcccgggc ggcgagctcc
     4741 tggtgcatgc cgactacgtg tacgaggaaa ataccatcga cgagaacgcc ccgaacttct
     4801 tcgaggaact gcgcgacctg ccgcgttatt tcgacgactc cgagatgctg aacgcacggc
     4861 tggcgtggat cagcggcgac gggcattacg agatcgcgct ctggggcaag aacctgctcg
     4921 acaaggagtt gatccagggc gtgcgcacca tcacgcgctc gagcttcggt accaccttcg
     4981 tcggcatcga ggatccgctg acctggggcc tcgaaggccg gtacacctgg tagggcgcat
     5041 gaaccaggcc cagagcagca ccctgtcgcc cgccgcgcgt cgcgtggcgc gcaccattct
     5101 gaacggcttc gagtcctact tcgccgaata ccagaacatc acgctgggcg cgaagcggcg
     5161 tttcgagacc gcgaactggc agggcgtgca cgaggccagc accgagcgca tcgacctcta
     5221 caagagcaag atcagtcgcg tggtgcgcct ggtgcgcgag gtgaccagcc aggacctgtc
     5281 caggctcgag ctctggcacg aggcgcgctc cgcctatgcg cagctggtca gcagtcattc
     5341 caattacgag atcgccgaga ccttcttcaa cagcatctat tgcaggatcc acgaccactc
     5401 gccgatccac gaccgctaca tcttcgtgaa gccctcgcgc gcgctcgagg acaagccgct
     5461 gcccgactac agcatctacg tgcgctacga gatggccggc gggctcgtcg acctggtgtc
     5521 gcgcatcctc gacgacttcg cgttcacggt gccctgggag gacaagcggc gcgacgtcat
     5581 gtccatcgtg aacctgctgc aggatcagct gctgcccgga ctggactgcc cgctcgaagg
     5641 gcttcgcgtc gagatgctgg agtcggtgtt ctaccgcaac aaggccgcgt acctcgtggg
     5701 gcgcgtgagc cacgccggcg gcgtgatccc ggtgatactg ccctgcctca acaacgagaa
     5761 gggcggcatc tacgtcgaca cggtgatcca cgaccaggat ggcgccagca tcatcttcag
     5821 tttcacgcgc tcgtacttca tggtcgacgc gccgatcccc tcgcgttacg tgcgcttcct
     5881 gctgtcgctg atgccgcaaa agggcgtcgc ggaactgtac aactcgatcg gcttcaacaa
     5941 gcatggcaag accgagttct accgcgacat gatccgccac atgaaggcga gcgaggatct
     6001 gtgcgtgatc gcgcccggca taaagggcat ggtgatgacg gtgttcacgc tgccctccta
     6061 cggcgtggtg ttcaaggtaa tcaaggaccg cttcgacccg cccaagcagg tcaccgagga
     6121 gatcgtgcgc gagaagtacc gcttcgtttc gcgcgccgat cgcgccgggc gcatggccga
     6181 cacgcaggag tacaagaact tcatcttcta ccgtaaccgg ttcagccagg agctgataga
     6241 tgagttgcag cgcgtcgcgc cgtcgaaact gtggatggac gagaagtgga tcatcatccg
     6301 ccacctctac gtcgagcggc gcatggtgcc gctgaacctc tacctgcgcg atgccggcga
     6361 ggcggaggtc tacgacgcga tggacgagta cggcaacgcg atc
//
LOCUS       DAORUQ010000171         6367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14308_length_6367_cov_6.493029, whole genome shotgun sequence.
ACCESSION   DAORUQ010000171 DAORUQ010000000
VERSION     DAORUQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14308_length_6367_cov_6.493029"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLW13_12950"
     CDS             complement(<1..996)
                     /locus_tag="PLW13_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HQY71334.1"
                     /translation="MRGEHSMDPVLGCSGRARRASLLGWLFAACAMLGASGAMAASDA
                     AEFFRDKRLTYIVPTKPGGGYDTYARLIARHLPKHLPVKAVEVRNVPGASHLIGVQQL
                     FAAPPDGLTLGTFNSGLVYAQINRTEGMNRDLRELSWIGKAASDARVFVVGAHTGIAS
                     VEQLRRGGRTLHLATSGKSSASSYETGMVIRLLGLDATPVHGFVGTEGELAVMRGDID
                     GLLGYHSALRDFVEQGHGRILFRIGAGPAELAALPELGSLLPAPEAQRLVELVAAQAS
                     LGRLTVAPPGVPPGRLAVLREAYLGTLADPELLREAAAMRLPIEALDGAAVQERIS"
     gene            complement(993..2288)
                     /locus_tag="PLW13_12955"
     CDS             complement(993..2288)
                     /locus_tag="PLW13_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQY71335.1"
                     /translation="MKNIALHWQILIAIVLAAIAGSLSGTEAGIGSLTFHGVYEFIGT
                     IFIKALKMIIVPLIFSSIVVGITGIGRAEDLGRLGAKTIGLYLVTGLLAVLVGLAFVN
                     TVRPGFENGAPVGAQLALQAGAQDVRQNLESSGGFGELVKMFERMVPENVVATASSNG
                     DLLALIFFALLFGFFIARVGDEHAEPLFNFWNAVFAVMMKITELIMRFAPIGVFGLVA
                     SVVAETGFDAVRPLGVFALTVVAALLTHALLVMPLLVWLLARVNPWRLYRAMSPALLT
                     AFSTASSSGTLPVTLDCMEKNAGISNRISSFVLPLGATVNMNGTALYECMAVMFLAQA
                     YGVELGFATQFLIVVSALITSIGVAGVPSASMVAIGMILTAVGIPVEAMGVLFVFDRV
                     LDMLRTSVNVLGDGATALVVARMQGEQTLLASPAQAHRA"
     gene            2432..3736
                     /locus_tag="PLW13_12960"
     CDS             2432..3736
                     /locus_tag="PLW13_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71336.1"
                     /translation="MTTRSFSIITGTLVALQAGALWLAGTQTAAAMPEFARRYNMSCA
                     ACHAAFPRLNKFGEEFAANNMRLPHWKDSTVKTGDSRLALPAYPPLAVRAQAYAQARD
                     GESVDPITGATDSATTDFQSPYLVKLLSSAPLSDNITYYFYAILAEKGTNGEALVEDA
                     WFRHADIFGTGIGMQLGQFQLSDLMFPREVRLSFQDFMPYRMAGITYDRGVLFDRDLG
                     PLELALGLSNGNGIEDNLNINSPGYRRPDKMFDNDSSKSVFGRVGTELGPVAAGVFGL
                     SAEQPSVATGNAGTISGDRDTDKRVVGLDLSGDFGGKTYWYAQYLWNSWDGFLDADPG
                     EDYRWDGGFAGVDYIPNERWAFSLLYNYADAGDLADTDTIYEGIDINSLSFTTSYYFM
                     RNVKGVIELNYDFLDQIDKDGEFWTGHLNKENYILFGFDTAF"
     gene            3738..4286
                     /locus_tag="PLW13_12965"
     CDS             3738..4286
                     /locus_tag="PLW13_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71337.1"
                     /translation="MHRPPCAARLRAGVAGLLGALLALPAPAQQPSLQQLRIESDPPG
                     AEVLLIGGSAGHTPLSVGERAIYPNDYSDEQAPLYGLVTLRRAGCATHVQRVTLEDIE
                     RGMHVTLDCDPAVATSAPDGQASPVATGKAGPVHATAPVASESVSQRRLRQLQVLQEL
                     LDEGLLSAAEEQRIRRELLRSP"
     gene            complement(4290..4718)
                     /locus_tag="PLW13_12970"
     CDS             complement(4290..4718)
                     /locus_tag="PLW13_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71338.1"
                     /translation="MQVEEKKRLLEQSLERVVEHVGDITRPTMARYYGRFPAAMEAFE
                     RLWPGNHAQLEGEMVERALYCLMYWFESPGEIEIMLGGSVLHHNDTLRVPPEWYAGLV
                     DATIDVIVATIPPGNGPELQVWAELRRELGGLVEQSRQLL"
     gene            complement(4785..5741)
                     /locus_tag="PLW13_12975"
     CDS             complement(4785..5741)
                     /locus_tag="PLW13_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71339.1"
                     /translation="MNNPFDIHPDFQSIKARTLSLKRWALALMRNLLAAVNALHRRKY
                     KAIVSRERIDGPDGHRIPVQIIRPEHPSVPCPALIYYHGGAFVMKHAPQHLENAVRYA
                     REAGCCVIFVEYRLAPVHPFPAGFDDCHAALRWALANAGTLGIDARRVAVGGDSAGGA
                     LAAAVAQRARHEDGIELCGQMLIYPVADVACTRPSTSAFVDVPPFKAASLASLWEVYF
                     RDPLAAGVPRYASPIHGELAGLAPAYVETAEFDMLHDQGHAYAEALIASGVEVARNDI
                     KGAVHGFDLLAADSEVSKAAMQSRIGFLRRIFGCAGTAPGTT"
     gene            complement(5774..>6367)
                     /locus_tag="PLW13_12980"
     CDS             complement(5774..>6367)
                     /locus_tag="PLW13_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71340.1"
                     /translation="RAPGFLARLEHDACRFLAHEAVIKRFLQGNRDLVALCHWNAHID
                     NAWFWRDAAGALQCGLIDWGRVRQMNVAYALWGSLSGATPEVWDRHLQEFLELFAREF
                     HAHGGPRLDVEELKLHLQLYVATMGLAWMLEAPARILFRLPEAAGASGPRDPVFRRSE
                     TARNQLHIFTVFLHLWQTQDFGGSLDRMLSRHGHPSA"
BASE COUNT         1127 a         2249 c         2010 g          981 t
ORIGIN      
        1 cgagatccgc tcctgcaccg ccgcgccgtc cagcgcttcg atcggcagcc tcattgccgc
       61 cgcctctcgc agcagctcgg gatcggcgag cgttccgaga tacgcctcgc gcagcaccgc
      121 cagccgcccg ggcggcacgc ccggcggcgc gacagtcagc cggccgagcg aagcctgcgc
      181 ggcgacgagc tcgacgaggc gctgcgcctc gggcgcgggc agcaggctgc cgagctcggg
      241 tagcgccgcg agctcggcgg gccccgcgcc gatgcggaac aggatgcggc catgcccctg
      301 ctccacgaaa tcgcgcagcg cgctgtggta gccgagcagg ccgtcgatgt cgccgcgcat
      361 caccgcgagc tcgccctcgg tgcccacgaa gccgtgcacc ggcgtggcat ccaggccgag
      421 cagccggatc accatgccgg tctcatagct ggaggcactc gacttgcccg acgtggccag
      481 gtgcagcgtg cgcccgcccc ggcgcagctg ctcgacggag gcgatgccgg tgtgcgcgcc
      541 gacgacgaag acgcgcgcat cgctggccgc cttgccgatc cagctcagct cgcgcaggtc
      601 gcggttcatg ccctcggtcc ggttgatctg ggcgtagacg agaccggaat tgaaggtccc
      661 cagcgtcaat ccgtcgggcg gcgcggcgaa cagctgctgc acgccgatca ggtggctggc
      721 gccgggcacg ttgcgcacct ccaccgcttt caccggcagg tgcttgggca ggtgccgcgc
      781 aatcagccgc gcgtaggtgt cgtagccgcc gcccggcttg gtcggcacga tgtaggtcag
      841 gcgcttgtcg cgaaagaatt ccgccgcatc ggaagcggcc attgcgccgc tcgcgccgag
      901 catcgcgcac gcggcgaaca gccagcccag cagcgacgcc cggcgcgcgc gaccggagca
      961 accgagaaca ggatccatcg aatgctcgcc cctcatgccc ggtgcgcctg cgccggcgag
     1021 gcgagcaggg tctgctcgcc ctgcatccgc gccaccacga gcgccgtcgc gccgtcgccc
     1081 agcacgttga cgctggtgcg cagcatgtcg agcacgcgat cgaacacgaa cagcacgccc
     1141 atcgcctcca ccggtatgcc gaccgccgtg aggatcatgc ctatcgccac catcgaggcc
     1201 gacggcacgc cggcaacgcc gatcgaggtg atcagcgcgc tcaccacgat caggaactgc
     1261 gtggcaaagc cgagctccac cccgtaggcc tgggcgagga acatcaccgc catgcattcg
     1321 tagagcgccg tgccgttcat gttcacggtc gcacccagcg gcagcacgaa gctcgagatg
     1381 cggttcgaga tgccggcatt cttctccatg cagtccagcg tgaccggcag cgtgccagag
     1441 gacgaggcgg tggaaaacgc cgtgagcagc gccggcgaca tcgcgcggta caagcgccat
     1501 gggttcaccc gcgccagcag ccagaccagc agcggcatca ccagcagcgc gtgcgtcagc
     1561 agcgccgcca ccaccgtgag cgcgaacacg ccgagcgggc gcactgcatc gaagcccgtt
     1621 tccgcgacga ccgaagccac cagcccgaac acgccgatcg gggcgaagcg catgatgagc
     1681 tcggtgatct tcatcatcac cgcgaacacc gcgttccaga aattgaacag cggctcggcg
     1741 tgctcgtcgc cgacgcgcgc gatgaagaag ccgaacagca gcgcgaagaa gatcaatgcc
     1801 agcagatcgc cgttgctgga ggccgtggcg accacgttct ctggcaccat gcgctcgaac
     1861 atcttcacca gttcgccgaa gccgccgctg gactcgaggt tctgccgcac atcctgcgcg
     1921 ccggcctgca gcgcgagctg cgcgccgacc ggcgctccat tctcgaaacc cggtcgcacc
     1981 gtgttcacga aggccagccc gaccagcacc gcgagcagcc ccgtgaccag gtacaggccg
     2041 atcgtcttcg cgcccagcct gccgaggtcc tcggcgcgcc cgatgccggt gatgccgacc
     2101 acgatcgagg aaaagatcag cggcacgatg atcatcttca aggccttgat gaagatcgtg
     2161 ccgatgaatt cgtagacgcc gtggaaggtg agcgacccga ttcccgcctc ggtgccgctc
     2221 agcgaaccgg cgatggcggc cagcacgatg gcgatcagga tctgccagtg cagcgcgatg
     2281 ttcttcatgc atgcttcccc tttgagcgcc ataagcatac acaagggcct gcgacgcgac
     2341 gaacccgcgt cggcttgatc aatcgggatt gacagatccg gagcctttcc atagttttcg
     2401 gtcacatgac gaaatgcagg gggcaacagg catgacaacg cgcagcttca gcattatcac
     2461 gggcactctc gtggcactgc aggccggggc gctctggttg gcgggcacgc aaacggccgc
     2521 ggcgatgcct gagttcgcgc gccgctacaa catgagctgc gccgcctgcc atgccgcctt
     2581 cccgcgcctg aacaagttcg gcgaggagtt cgccgccaac aacatgcgcc tgccgcactg
     2641 gaaggacagc accgtcaaga ccggcgacag ccgcctcgcg ctgccggcct atccgccgct
     2701 cgcggtgcgc gcgcaggcct acgcgcaggc gcgcgacggc gagtccgtgg acccgatcac
     2761 cggcgcgacc gactcggcca ccaccgactt ccagtcgccc taccttgtga agctgctctc
     2821 gagcgcgccg ctcagcgaca acatcactta ctacttctac gccattctcg ccgagaaggg
     2881 caccaacggc gaggcgctgg tcgaggatgc ctggttccgc cacgccgaca tcttcggcac
     2941 cggcatcggc atgcagctgg ggcagttcca gctctccgac ctgatgtttc cgcgcgaggt
     3001 gcggctcagc ttccaggact tcatgcccta ccgcatggcc ggtatcacct acgaccgcgg
     3061 cgtgctgttc gaccgcgacc tcggcccgct cgagctcgcg ctcggtctca gcaacggcaa
     3121 tggcatcgag gacaacctga acatcaacag tcccggctac cgccgcccgg acaagatgtt
     3181 cgacaatgac agctcgaagt cggtgttcgg tcgtgtcggt accgagctcg gaccggtggc
     3241 cgcaggggtg ttcggcctga gcgccgaaca gcccagcgtg gcaacaggca acgccggaac
     3301 gatcagcggc gatcgcgaca ccgacaagcg tgtcgtgggc ctcgatctct ccggggattt
     3361 cggcggcaag acgtactggt acgcccagta cctgtggaat tcctgggacg gcttcctcga
     3421 cgcagacccc ggcgaggact accgctggga cggtggcttc gccggcgtcg actacatccc
     3481 caacgagcgc tgggcgttct cgctgctcta caactatgcc gatgccggcg atctcgccga
     3541 taccgacacc atctacgaag gcatcgacat caacagcctc agcttcacga cctcgtacta
     3601 cttcatgcgc aatgtgaagg gcgtgatcga gctcaactac gacttcctcg atcagatcga
     3661 taaggacggc gaattctgga ccggtcacct gaacaaggag aactacatcc tgttcggctt
     3721 cgacaccgcg ttctgagatg catcgccccc cttgcgccgc gcggctgcgc gccggcgtcg
     3781 ccggcctgct cggcgcgctg ctcgcgctgc ccgccccggc gcaacagccg agcctgcagc
     3841 agctgcgcat cgaatccgat ccccccggcg ccgaagtgct gctgatcgga ggcagtgccg
     3901 ggcacacgcc gctcagtgtg ggcgagcgcg cgatctaccc caacgactac agcgacgagc
     3961 aggcgccgct gtacggcctg gtgacgctgc gccgtgccgg ctgtgccacc catgtgcagc
     4021 gcgtcacgct cgaggacatc gagcgcggca tgcatgtgac gttggactgc gaccctgcag
     4081 tcgccacatc ggcgcccgac ggccaggcgt caccggtggc gacgggcaaa gccggtccgg
     4141 tgcacgccac tgctcccgtc gccagcgaat cggtttccca gcgtcgactg agacagctgc
     4201 aggtgctgca ggaactgctc gacgagggat tgctcagcgc cgcggaggaa cagcgaatcc
     4261 ggcgcgagtt gctgcgatcg ccatagcgct cacagcaact gacgactctg ctcgaccagt
     4321 ccgccgagtt cacggcgcaa ctcagcccac acctgcagtt ccggaccgtt cccgggcgga
     4381 atcgtcgcga cgatgacatc gatcgtcgca tccaccaggc ccgcgtacca ttcgggtggc
     4441 acacggagcg tgtcgttgtg atgcaacacg gacccgccga gcatgatctc gatctcgccc
     4501 ggcgattcga accagtacat caggcagtac agagcccgct cgaccatttc gccctcgagc
     4561 tgcgcgtggt ttcccggcca gagcctctcg aacgcttcca tggcagccgg gaagcgtccg
     4621 taataccgcg ccatcgtggg ccgggtgata tcgccgacat gctcgaccac tcgttccagg
     4681 ctttgttcga gcagccgctt tttttcctcg acctgcatga ccgccctcct cgtaccgggt
     4741 gatgtggacg tgcccgatgg tatctgccgg cgcccggccg atcgtcacgt cgtgccgggc
     4801 gccgtgcctg cacagccgaa tatcctgcgc aggaacccga tccggctctg cattgccgcc
     4861 ttcgagacct cgctgtccgc tgccagcaga tcgaaaccgt gcaccgcgcc cttgatgtcg
     4921 ttccgcgcca cctcgacccc gctggcgatc agggcctcgg cataggcgtg gccctggtcg
     4981 tgcaacatgt cgaattccgc cgtctcgacg taggccgggg caaggccggc caattcgcca
     5041 tggatgggcg atgcgtaacg cggtacgccg gccgccaacg gatccctgaa atacacctcc
     5101 catagtgacg caagcgatgc cgccttgaat ggcggcacat cgacgaaggc cgaggtcgag
     5161 ggccgcgtgc atgccacgtc ggcgaccggg tagatcagca tctgcccaca caactcgata
     5221 ccgtcctcgt gccgcgccct ttgcgccacc gcggcggcca gtgcgccgcc tgcgctgtcg
     5281 ccgcccaccg ccacccgccg cgcatcgatg ccgagtgtcc cggcattggc caaagcccag
     5341 cgcaaagccg cgtggcaatc gtcgaatccc gccggaaacg ggtgcacggg tgcgagccgg
     5401 tattcgacga agatcacgca gcagcccgct tcgcgcgcgt agcgcacggc attctcgaga
     5461 tgctgcggcg catgcttcat cacgaatgcg ccgccgtgat agtagatcag tgccgggcac
     5521 gggacggacg ggtgctccgg ccggatgatc tgcaccggga tgcggtggcc gtcgggacca
     5581 tcgatccgct cgcgcgacac gatcgccttg tacttgcggc gatgcagcgc gttcacggcg
     5641 gccagcagat tgcgcatcag agccagcgcc cagcgtttca gcgagagcgt gcgcgccttg
     5701 atcgactgga aatcggggtg gatgtcgaag gggttgttca cttgccgtta ccgtgatgaa
     5761 tgtaggtcgg cattcatgcc gacgggtgcc catgcctgct cagcatgcga tccagactgc
     5821 caccaaagtc ctgcgtctgc cacaggtgca ggaacaccgt gaagatgtgc aactggttgc
     5881 gtgcggtctc gctcctgcga aagaccggat cgcgcggccc ggacgcgccg gccgcttccg
     5941 gaaggcggaa caggatccgc gccggcgcct ccagcatcca cgcgagaccc atcgtcgcga
     6001 catacaactg caggtggagc ttgagttcct ccacatccag gcgcggtccg ccgtgcgcgt
     6061 ggaactcgcg cgcgaacagc tccaggaact cctgcaggtg ccgatcccag acctcgggag
     6121 tggccccgct caggctcccc cacagcgcgt aggccacgtt catctgcctg acccgccccc
     6181 agtcgatcag cccgcactgc agcgcgcccg ccgcatcgcg ccagaaccat gcgttgtcga
     6241 tatgggcgtt ccagtggcac agcgcgacga ggtcgcgatt gccctgcagg aagcgcttga
     6301 tcaccgcttc gtgcgcgagg aagcggcagg cgtcgtgttc cagcctcgcc aggaaaccgg
     6361 gcgcgcg
//
LOCUS       DAORUQ010000172         6343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14395_length_6343_cov_6.310115, whole genome shotgun sequence.
ACCESSION   DAORUQ010000172 DAORUQ010000000
VERSION     DAORUQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14395_length_6343_cov_6.310115"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLW13_12985"
     CDS             complement(<1..885)
                     /locus_tag="PLW13_12985"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQY71341.1"
                     /translation="MTALFNLGFRPFFLGAGVFASLSMAAWLTIYAAWYQPAMQGISA
                     SQWHAHEMIYGYTMAVIAGFLLTAIRNWTGIQTLQRTPLALLFACWAVARLALLAGTT
                     FIAIAAAADLLFAALLIAACAVPIVRARQWRQMAVLSKLVLLGIGNALFYAGAFGLLD
                     EGVRWSLYGAFYVVIALILTMARRLVPLFTASGVEPKVILRNSLWLDMSSLVLLVVFW
                     IAEVFLQRHGIAAGASALMFLVNATRLAFWYTPGIWSRPLLWSLHGAFALITAAFLLH
                     ALSAVTTLNPSLALHAFAV"
     gene            complement(984..3077)
                     /locus_tag="PLW13_12990"
     CDS             complement(984..3077)
                     /locus_tag="PLW13_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71342.1"
                     /translation="MRILKPLACALATVGACATPALAAPCAGTACDGTLLEEIVVTAR
                     RAPRIVLDASETIGVIDREQLARSTAYGLADAMRSMPGVQVADSGQPGLKRVRIRGEE
                     SRRTAILIDGQEITDHWEVGTPLSLNPAMVERIEVVRGSGSVLYGPRALSGVVNFITR
                     KGGTEPLQLEASSGWNSATRGEERFASFYGDAGGLEYRVAAADSEHGKRYTPQGKIEN
                     TAFDNRSAYAWAGRDFGTQRLELAYDRYESATGVFVEEEERTAFPLTDFRIDVPQRDR
                     EKLALFYEWHEVASFLPVIKANVHRQWNQRRFDTFSATLVPGARPVATERSVFSDASL
                     EVDDGVLQLDWTPHADHYIVSGLQYTEERVNQERHVDTVTNGITGRPENIHDEAAIET
                     VALFAQDEWRLAERTTLTLGARQYRVEGRLEETNRAGLAPSTQDDAQLITSLGLNHEL
                     ADNVVLRANVAQGYVYPSLLQFAIGAYAASRFVNPNPDLDPETALSWEGGLRLRAAGW
                     VFDAGVFRTASEDYIDHVFCTPTDGCLSARDKIYVNIGESDAQGLELYTARAEGDLRW
                     RPYASLTWMKRENRYENFVTEDSGTPSLSGTLGVTWEPLFATAMDGWLDLGLRGESAS
                     KREEPSSSGLSTLDEDDAWLTLNLSGGLRFGEAQRLGLVVELENLGDATYSTSAENLL
                     APGRSVSARVTFALD"
     gene            complement(3065..3487)
                     /locus_tag="PLW13_12995"
     CDS             complement(3065..3487)
                     /locus_tag="PLW13_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71343.1"
                     /translation="MSRGSTAVAVARLALALLAMSGGAFAADADMVNERPPVAPEQLE
                     QHWGVDCARLRRELLAAGTVSAPDAPPAPTHEDTQARITRWHAGLRLCAAIHNAPGDR
                     ETPHCPDYARAARALDENATGRDDTMRAQIQEALRCES"
     gene            3596..5194
                     /locus_tag="PLW13_13000"
     CDS             3596..5194
                     /locus_tag="PLW13_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQY71344.1"
                     /translation="MNRIDPRRLFRRAAALFAAVMVCLSLDAAPASRLETLDTAAVRK
                     VFAGADRLGAPLDVAPVRAVFAGGEFQGWVFLSDDVHPVPAYSGKPITVLVGLRPDAR
                     IAGASIMAHEEPILVIGVSDADLQRFIGQYAGIGATEKVRVGALGREGYRGIDAITGA
                     TITVMVLNQSLMRSAQEVAAALGLPLAPGGAAPGAEPAAAPLPVTPVVAAADASASEP
                     EWLQLWQERRLRVAVLLAALGLLVAMLVFQDWLVRRPRLFRRLRTGYLLFTVLFVGFY
                     SMGQLSIVNMLAFGRVMAGGFSWDTLLLEPVTFLLWGFVAVTIILWGRGVYCGWLCPF
                     GALQELIHAAAERFKLPKWEFPAMVHERLWAIKYLILIALFGLSLESMARAATVAEVE
                     PFKTVFALHFAREWYFTLYALGLLAISAVNSKFYCKYLCPLGAALSFFTRFRIFDWLR
                     RRRECGNPCQTCAAQCQIGAIRPTGEIIENECHYCLECQVTYLDDHRCPPLVEKRKRR
                     ETREVRAAEFAAVRILDLDHRNSD"
     gene            5353..>6343
                     /locus_tag="PLW13_13005"
     CDS             5353..>6343
                     /locus_tag="PLW13_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQY71345.1"
                     /translation="MKTFRSKLISVACALASVTLLGNAVVAQEKSDHPGTVGEDVRYR
                     GAPAMQPEVKQVTSPGAPPVTEAEFARATKIYFERCAGCHGVLRKGATGKPLTPDITQ
                     QRGTEFLKILINMGSPAGMPNWGTSGDLSAEEIDIMARFLQHEPPTPPEWGMKEMKES
                     WKVLVPVDQRPTKQMNNFNLDNIFAVTLRDSGEVALIDGDSKKIIKIIKTGYAVHISR
                     ASNSGRYVYTIGRDAKIDMIDLWMAEPQVVATIKIGLEARSVETSKYKGYEDKLAIAG
                     AYWPPQYVIMDGPTLEPKKIVSTRGMTVDTQEYHPEPRVAAIVASHEHPEFIVN"
BASE COUNT          988 a         2128 c         2184 g         1043 t
ORIGIN      
        1 gaccgcgaag gcgtgcagtg cgagcgaggg attcagcgtc gtcaccgcac tcagcgcatg
       61 cagcaggaaa gccgcagtga tcagcgcgaa ggccccgtgc aggctccaca gcagcggccg
      121 gctccagatt ccgggcgtgt accagaacgc cagccgcgtg gcgttgacca ggaacatcag
      181 cgcgctcgcg ccggcggcga tgccgtgacg ctgcaggaag acctcggcga tccagaacac
      241 caccagcagc accaggctcg acatgtcgag ccacagcgaa ttacgcagta tcaccttggg
      301 ctcgacgccg cttgccgtga acagcgggac caggcgccgg gccatcgtca ggatcagcgc
      361 gatcaccacg tagaacgcgc cgtacaggct ccagcgcacc ccctcgtcga gcaggccgaa
      421 cgcgccggcg tagaacagcg cgttgccgat gcccagcagc accagcttcg acagcaccgc
      481 catctgccgc cactggcgcg cgcgcacgat cggcaccgcg cacgcggcga tcagcagcgc
      541 ggcgaacagc aggtccgcgg cggccgcgat ggcaatgaac gtggtgccgg cgagcagcgc
      601 gaggcgcgcc accgcccagc aggcgaacag cagtgccagc ggcgtgcgct gcagcgtctg
      661 gatcccggtc cagttgcgga tggcggtcag caggaagccc gcgatcacgg ccatggtgta
      721 gccgtagatc atctcgtgcg catgccactg gctggcgctg atgccctgca tcgcgggctg
      781 gtaccacgcg gcgtagatcg tcagccatgc ggccatcgac agcgaggcga aaacgccggc
      841 gccgaggaag aaggggcgaa aacccaggtt gaacagtgcg gtcatgcggt gccttcctgc
      901 agctatcgtg attgcccccg tgggtccgaa tttattcgga cgattgtccg gctgaagccg
      961 gacccacggg tcggacccac gggtcagtcg agcgcgaacg tcacgcgggc cgagacgctg
     1021 cgccccggcg ccagcaggtt ctcggccgag gtcgagtagg tcgcgtcgcc caggttctcg
     1081 agttccacga cgaggccgag gcgttgcgcc tcgccgaacc gcaggccgcc gctcaggttg
     1141 agcgtgagcc aggcgtcgtc ctcgtcgagc gtcgacagcc cgctgctcga tggctcctcg
     1201 cgcttcgagg cgctctcgcc gcgcagcccg aggtcgagcc agccgtccat ggctgtggcg
     1261 aacagcggct cccacgtcac gccgagcgtg ccgctcaggc tcggggttcc cgagtcctcg
     1321 gtcacgaagt tctcgtagcg gttctcgcgc ttcatccagg tgaggctcgc gtacgggcgc
     1381 cagcgcagat cgccttcggc gcgcgcggtg tagagctcca ggccctgggc gtcggactcg
     1441 ccgatgttca cgtagatctt gtcgcgtgcc gacaggcagc cgtcggtcgg tgtgcagaag
     1501 acgtggtcga tgtagtcctc ggacgcggtg cggaacacgc cggcgtcgaa cacccagccc
     1561 gccgcgcgca ggcgcagccc gccttcccac gacagcgcgg tctcggggtc cagatcgggg
     1621 ttcgggttca cgaagcgcga ggccgcgtag gcgccgatcg cgaactgcag cagcgagggg
     1681 tagacgtaac cctgcgcgac atttgcgcgc agcaccacgt tatccgcgag ctcgtggttc
     1741 agcccgaggc tcgtgatcaa ctgcgcgtcg tcctgcgtcg atggcgccag tcccgcgcgg
     1801 ttcgtctcct cgagccggcc ctctacccgg tactggcgcg cgcctagcgt gagcgtcgtg
     1861 cgctcggcca gccgccattc gtcctgcgcg aacagcgcca ccgtctcgat cgccgcctcg
     1921 tcgtggatat tctcgggccg ccccgtgatg ccgttggtca cggtgtcgac gtggcgctcc
     1981 tggttcacgc gctcctcggt gtactgcaac ccgctgacga tgtagtggtc ggcatgcggc
     2041 gtccagtcga gctgcagcac gccgtcgtcc acctcgaggc tcgcatcgct gaagacgctg
     2101 cgctcggtcg cgacgggcct cgcgcccggg accagcgtgg cgctgaaggt atcgaaccgg
     2161 cgctggttcc actggcgatg gacgttcgcc ttgatcacgg gcaggaaact cgcgacctcg
     2221 tgccattcgt agaacagcgc gagcttctcg cggtcgcgct gcggcacgtc gatgcgaaag
     2281 tcggtgagcg ggaaggcggt gcgctcctct tcctccacga agaccccggt cgccgattcg
     2341 tagcggtcgt aggcgagctc gaggcgctgc gtgccgaaat cccgtcccgc ccaggcgtag
     2401 gcgctgcggt tgtcgaaggc ggtattctcg atcttgccct gtggcgtata gcgcttgccg
     2461 tgctcgctgt cggccgcggc cacgcggtac tcgaggccgc cggcatcgcc gtagaagctc
     2521 gcgaagcgct cctcgccgcg cgtcgcggag ttccagccgc tgctcgcctc cagttgcagc
     2581 ggctcggtgc cgcccttgcg cgtgatgaag ttcaccacgc cgctcagcgc gcgcggcccg
     2641 tacagcaccg aacccgagcc gcgcaccacc tcgatgcgtt ccaccatcgc cgggttcagg
     2701 ctgagcggcg tgccgacctc ccagtggtcg gtgatctcct ggccgtcgat gagtatcgcg
     2761 gtgcggcgcg attcctcgcc gcggatgcgc acgcgcttca ggcccggctg cccggagtcg
     2821 gcgacctgca cgccgggcat gctgcgcatg gcgtcggcga gcccgtaagc ggtggaacgt
     2881 gcgagctgct cgcgatcgat cacgccgatg gtctcgctgg cgtcgagcac gatgcgcggc
     2941 gcgcggcgcg cggtgaccac gatctcctcc agcagcgtcc cgtcgcaggc cgttcccgca
     3001 cacggcgccg cgagggccgg ggtcgcgcag gcgccgaccg tggcgagcgc gcaggccagt
     3061 ggcttcagga ttcgcatcgc agggcttcct gtatctgcgc tcgcatcgtg tcgtcgcggc
     3121 ccgtcgcatt ttcgtcgagc gcccgcgcgg cgcgggcata gtcgggacag tgcggggtct
     3181 cccggtcgcc gggagcgttg tggatagccg cgcacaggcg caggcccgca tgccagcgcg
     3241 tgatgcgcgc ttgcgtgtcc tcgtgcgtcg gcgcgggcgg ggcatcgggc gccgataccg
     3301 tgccggctgc cagcaactcg cgtcgcagcc gcgcgcagtc gacgccccag tgctgctcga
     3361 gctgctcggg cgcgaccggt gggcgctcgt tgaccatgtc cgcgtcggcg gcgaacgcgc
     3421 cgccgctcat ggccagcagc gccagcgcaa ggcgtgccac ggcgacggcg gtcgatcctc
     3481 ggctcaagat gcatttcccg tcgggtggtt gtatacaccg cgcatgatcc gctgattcgg
     3541 gcaacgaatc cagttggctg gcgcgcggcg ccttatggcg tatgatcgtc cgcgtatgaa
     3601 tcgcatcgat ccgcgccgcc ttttccgtcg ggctgccgcc ctgtttgcgg cagtgatggt
     3661 ctgcctgtcg ctcgatgcgg cccccgccag ccggctcgaa accctcgata ccgctgccgt
     3721 gcgcaaggtc tttgccggag ccgatcgcct cggcgcgccg ctcgacgtcg cgccggtgcg
     3781 cgcggtattc gcgggcggcg agttccaggg ctgggtgttc ctcagcgacg acgtgcaccc
     3841 ggtgccggcg tattcgggca agccgatcac ggtgctggtc gggctgcggc ccgatgcgcg
     3901 catcgccggc gcgagcatca tggcgcacga ggagccgatc ctggtgatcg gcgtcagcga
     3961 cgccgacctg cagaggttca tcggacagta cgccggcatc ggcgccaccg agaaggtgcg
     4021 cgtgggcgcc ctcggacgcg aaggctaccg cggcatcgac gcgatcaccg gcgcgaccat
     4081 cacggtgatg gtgctgaacc agagcctgat gcgctccgcg caggaagtgg ccgcggctct
     4141 cggcttgccg ctcgccccgg gcggtgccgc gccgggcgcg gagcccgccg cggcgccgct
     4201 gccggtcacg ccggtggtag ccgctgccga cgcgtccgcc agcgaacccg aatggctgca
     4261 gctctggcag gagcgccgct tgcgcgttgc ggtgttgctt gccgcgctcg gactgctcgt
     4321 cgcgatgctg gtgttccagg actggctggt gcggcgaccg cggctgttcc gccgcctgcg
     4381 cacgggctac ctgctgttca cggtgctgtt cgtcggtttc tacagcatgg gacagctgtc
     4441 gatcgtcaac atgctcgcct tcggccgcgt catggcgggc gggttctcgt gggatacgct
     4501 gctgctggag ccggtcacgt tcctgctgtg gggcttcgtg gcggtcacca tcattctgtg
     4561 ggggcgcggg gtgtactgcg gctggctctg tcccttcggc gcgctgcagg agctgatcca
     4621 tgccgccgcg gagcgcttca agctgccgaa gtgggagttc cccgcgatgg tccacgagcg
     4681 cttgtgggcc atcaagtacc tcatcctgat cgcgctgttc gggctgtcgc tggagtcgat
     4741 ggcgcgcgcg gcgaccgtcg cggaagtgga gccgttcaag accgtgttcg cgctgcactt
     4801 cgcgcgtgag tggtacttca cgctgtacgc gctgggcctg ctggccatca gcgcggtgaa
     4861 cagcaagttc tactgcaagt acctgtgccc gctgggggcg gcgctgagct tcttcacgcg
     4921 ctttcgcatc ttcgactggc tgcgccggcg ccgcgagtgc ggcaacccct gccagacctg
     4981 cgccgcgcaa tgccagatcg gcgcgatccg gcccacgggc gagatcatcg agaacgaatg
     5041 tcactactgc ctggaatgcc aggtcaccta tctcgacgat caccggtgtc cgcccctggt
     5101 cgagaagcgc aagcgccgcg agacgcgcga ggtgcgggcc gccgagttcg cggcggtacg
     5161 aatacttgat ctggatcatc gtaattccga ttgaaacgct cggggatgga gcttgccgat
     5221 ccgagtgcca tccgttattg atatggcgca ataaatccga caggaccact cgggtacatt
     5281 gcgttcacgg ggatcagcgt tgaaaacacc aatacggttc ctcgattccg aaccggcaac
     5341 aggagcgatg caatgaagac cttcaggagc aagttgatca gtgtggcgtg tgccttggcc
     5401 tcggtgacgt tgctgggcaa tgcggtggtg gcccaggaaa aatccgatca ccccggcacg
     5461 gtcggtgagg acgtgcgcta tcgcggcgca ccggcgatgc agcccgaggt gaagcaggtg
     5521 accagcccgg gggctccccc ggtgacggag gccgaattcg cgcgggcgac caagatctat
     5581 ttcgagcgtt gcgccggctg tcatggcgtg ctgcgcaagg gcgccacggg caagccgctg
     5641 acccccgaca tcacgcagca gcgcggcacc gagttcctga agatcctgat caacatgggc
     5701 tcgccggccg gcatgccgaa ctggggcacc tcgggtgatc tctcggccga agagatcgac
     5761 atcatggcac gcttcctcca gcacgagccg cccacgccgc ccgaatgggg catgaaggag
     5821 atgaaggaaa gctggaaggt gctggtgccg gtggaccagc gcccgacgaa gcagatgaac
     5881 aacttcaacc tcgacaatat cttcgcggtg acgctgcgcg acagcggcga ggtggcgctg
     5941 atcgacggcg actcgaagaa gatcatcaag atcatcaaga ctggctacgc ggtgcacatc
     6001 tcgcgtgcct cgaactccgg gcgctacgtg tacaccatcg gtcgtgacgc gaagatcgac
     6061 atgatcgacc tgtggatggc cgaaccccag gtggttgcca cgatcaagat cgggctcgag
     6121 gcgcgttcgg tcgagacgtc caagtacaag ggctacgagg acaagctcgc gattgccggc
     6181 gcctactggc cgccgcagta cgtgatcatg gacggcccga cgctggagcc gaagaagatc
     6241 gtctccacgc gcggcatgac ggtcgacacg caggaatacc acccggagcc gcgcgtggcc
     6301 gcgatcgtgg cctcgcacga gcaccccgag ttcatcgtca acg
//
LOCUS       DAORUQ010000173         6279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14623_length_6279_cov_5.521690, whole genome shotgun sequence.
ACCESSION   DAORUQ010000173 DAORUQ010000000
VERSION     DAORUQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14623_length_6279_cov_5.521690"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLW13_13010"
     CDS             complement(<1..446)
                     /locus_tag="PLW13_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQY71346.1"
                     /translation="MRRVVVTGMGIVSCLGNTPEAVTASLREGRSGIKFIPEYAEMGF
                     RSHVAGRPQVDFAAEIDRKQLRFMGDAAAFAYIAMKQAIADAGLTEAEVSNERTGIIA
                     GSGGASSSNQVEAADIMREKGVKRVGPYRVTQTMASTVSACLATPF"
     gene            complement(465..1004)
                     /gene="fabA"
                     /locus_tag="PLW13_13015"
     CDS             complement(465..1004)
                     /gene="fabA"
                     /locus_tag="PLW13_13015"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HQY71347.1"
                     /translation="MPARREPSTQHSFSKEELIACGRGTLFGPGNAQLPVDNMLMLDR
                     ITHISGEGGAHGKGEIIAELDIHPGLWFFGCHFVGDPVMPGCLGLDAMWQLVGFFLGW
                     RGNPGRGRALGAGEIKFTGQVLPEATKVVYHIHLKRVMERKLVMGIADGELSVDGKVI
                     YTAHDLRVGLFTTAADALG"
     gene            1179..2528
                     /locus_tag="PLW13_13020"
     CDS             1179..2528
                     /locus_tag="PLW13_13020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQY71348.1"
                     /translation="MRIAVIGTGYVGLVTGACFAEMGNHVVCVDNDPAKLARLQAGEI
                     PIFEPGLQPVVEQAVQAGLLTYSADLAAAVGAAEYIFIAVGTPPGEDGSADLRYVLAV
                     ARDIGRHLCRYAVVVDKSTVPVGTADKVRAAISEELAARGASIGFDVVSNPEFLKEGA
                     AVSDFRSPDRVIVGTDSEKARRRMTELYGPLLRNHDRMLFMGVRDAEMTKYAANAMLA
                     TRISFMNEIANLCDRLGVDVENVRKGIGSDSRIGYSFIYPGCGYGGSCFPKDVKALVQ
                     SSRACDFEPSLLQAVEARNERQKRVLFDKVVAHFGADLNGRRFGVWGLSFKPNTDDMR
                     EAPSLVLIEQLLAAGARVAAYDPVVGNDGHAGMPRDWLESGRVELSKYQYDALREADA
                     MILVTEWKRFRQPDFDAMRDLLLTPVIFDGRNQYDPVLMSDYGFTYYGIGRSNVAAG"
     gene            2695..4113
                     /locus_tag="PLW13_13025"
     CDS             2695..4113
                     /locus_tag="PLW13_13025"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HQY71349.1"
                     /translation="MLIPVLLSGGVGSRLWPVSRELYPKQFLPLADEKLSLFQQTLER
                     LGGMPALGEPVVVCNEEHRFLVAQQLRDMGHGGASIILEPIGRNTAPAIALAAIDALR
                     QTEDAVLLVLPADHVIRDCDALQRAAQTGMALAGAGHLVTFGIVPQAPETGYGYIRRG
                     AALDDQVYAVSHFAEKPDLETANAYVASGEYFWNSGMFLFRADRYLEELDKYAPQMLA
                     ACRKAYEQASDDLDFIRVHKESFTACPSDSIDYAVMERTDRAAMVPLDCGWNDVGSWG
                     ALWQTGTPDARGNVSRGDVILRNVSNAYVRAESRLVAAVGLEDVVIVETADAVLVANK
                     DSSQDVKHIVAHLKLEGREEALLHTRVYRPWGSYESLVESERFQVKRIIVNPGQQLSL
                     QMHHHRAEHWVVVKGTARVTHGEQETMLGEDQSTYIPLGTKHRLANPGVIPLEIIEVQ
                     TGSYLGEDDIVRFNDVYGRAGS"
     gene            4220..5452
                     /locus_tag="PLW13_13030"
     CDS             4220..5452
                     /locus_tag="PLW13_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71350.1"
                     /translation="MIRLLASLFTLALVALLGMAVLLFSAIDTRPLVDRGETISTNAI
                     DQAPKLLLANDPRRLRPGEERSALIPATLLDEGVNYVATQLLHCRGALSLQDDSAEIR
                     LSLPLPGTPGYLNLRATVPEAAGEPRIAALRVGDVQIPAVFADQALDLALNSLGYGQQ
                     WRMARAAVRELAFDPARGMVDVRYAWDPALLDSARAAALDPADVDRIREAQAAFATLL
                     EGKPRGERVTLAEVLAPLMSGNGQGREQRRAALLVLASSLAGGKLANIVPEAASWPQP
                     PRVAITLRGRLDSAQHFVISAALAAWAGEPVATAIGLYKELEDARRGSGFSFADLAAD
                     RAGTRFGELVAANAARLDEVLAGTPGDAELLPPLDGLPEFLPEAEFRRRYGGPGEPAY
                     EKVADEIERRLSRLALYR"
     gene            5807..>6279
                     /locus_tag="PLW13_13035"
     CDS             5807..>6279
                     /locus_tag="PLW13_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY71351.1"
                     /translation="MIKKCLFPVAGYGTRFLPATKSMPKEMLPIVNKPLVQYGVEEAL
                     DAGLFEIAFVTGRGKRTINDHFDISYELEHQIAGTDKEQTLVGIRNVIDRAVFSTTRQ
                     REMKGLGHAILTGQTLIGDEPFGVILADDLCFNEGGDGVMAQMVKIYKQVRCSIVA"
BASE COUNT         1035 a         2143 c         2061 g         1040 t
ORIGIN      
        1 ttgaacggcg tggccaggca ggccgacacg gtgctggcca tggtttgcgt cacgcgatac
       61 ggtccgacgc gcttcacgcc cttctcgcgc atgatgtcgg ccgcttccac ctggttcgac
      121 gacgaggcgc cgccggaacc ggcgatgatg ccggtgcgct cgttggagac ctcggcctcg
      181 gtgagccccg cgtccgcgat ggcctgcttc atcgcgatgt aggcgaaggc cgccgcatcg
      241 cccatgaagc gcagctgctt gcggtcgatc tcggccgcga aatccacctg ggggcggccc
      301 gccacgtggc tgcgaaagcc catctcggca tattccggga tgaatttgat gcccgatcgg
      361 ccctcgcgca gcgacgcggt gacggcctcc ggggtgttgc ccaggcacga gacgattccc
      421 atgccggtga cgacgactct gcgcattgcg atttcctccg tgacctagcc caaagcatcc
      481 gcggccgtgg tgaacagacc cacgcgcagg tcatgcgcgg tgtagatcac cttgccgtcg
      541 accgacaact cgccgtcggc gatgcccata accagcttgc gttccatcac ccgcttcagg
      601 tggatgtggt agacgacctt cgtcgcctcg ggcagcacct gcccggtgaa cttgatctcg
      661 cccgcgccca gcgcgcgccc gcggcccggg ttgccgcgcc agccgaggaa gaagcccacc
      721 agctgccaca tcgcgtccag tcccaggcag cccggcatca ccgggtcgcc cacgaagtgg
      781 cacccgaaga accacagccc cgggtggatg tcgagctcgg cgatgatctc gcccttgccg
      841 tgcgcaccgc cctcgccgct gatatgcgtg atgcggtcga gcatcagcat gttgtcgacc
      901 ggcagctgcg cgttgccggg cccgaacagg gtgccgcgtc cgcaggcgat gagttcttcc
      961 ttgctgaagg aatgctgggt cgacggctcg cgccgcgcgg ggatggtgct caacagcggg
     1021 atctctgggg gagtgtgcaa gtgcgcgaat tgtagtgagt ttgcccatgg ccgtcacctg
     1081 cgccagccgg cgtccggatc cgcagggccg atctgacaag cagcggtcca tccactagac
     1141 tttgggccgg caaacagcag cgcaaggaat ctcccagggt gcgaatagca gtcatcggca
     1201 cgggatacgt gggcctggtc acgggtgcct gtttcgcgga aatgggcaac cacgtggtgt
     1261 gcgtcgacaa cgaccccgcc aagctcgcgc gcctgcaggc cggcgagatc cccattttcg
     1321 agccgggcct gcagccggtg gtggagcagg cggtgcaggc cgggctgctt acatacagcg
     1381 ccgacttggc cgccgcggtg ggcgcggccg agtacatctt catcgcggtc ggcacgccgc
     1441 cgggcgagga cggctcggcg gacctgcgct acgtgctcgc ggtggcgcgc gacatcgggc
     1501 gccacctgtg ccggtacgcg gtcgtggtcg acaagtcgac ggtgccggtg ggcaccgccg
     1561 acaaggtgcg cgccgccatc agcgaggagc tggccgcgcg tggcgcgagc atcggcttcg
     1621 acgtggtcag caaccccgaa ttcctcaagg agggcgcggc ggtgagtgac ttccgcagcc
     1681 ccgatcgcgt catcgtgggc accgattccg agaaggcgcg ccggcgcatg accgaactct
     1741 acggtccgct gctgcgcaac cacgaccgca tgctgttcat gggcgtgcgt gatgccgaga
     1801 tgaccaagta cgccgccaac gcgatgctgg ccacgcggat ctcgttcatg aacgagatcg
     1861 ccaacctgtg cgaccgcctc ggcgtggatg tcgagaacgt gcgcaagggc atcggttccg
     1921 acagccgcat cggctactcg ttcatctatc ccggttgcgg ctacggcggc tcctgtttcc
     1981 cgaaggacgt gaaggcgctg gtgcagagca gccgtgcctg cgatttcgag ccctcgctgc
     2041 tgcaggccgt ggaagcgcgc aacgagcgcc agaagcgcgt gctgttcgac aaggtcgtgg
     2101 cgcatttcgg cgccgatctg aacgggcggc gcttcggcgt gtggggtctt tccttcaagc
     2161 ccaacaccga cgatatgcgc gaggcgccgt cgctggtgct gatcgaacag ctgctcgcgg
     2221 ccggcgcccg tgtcgcggcc tacgacccgg tggtcggcaa cgacggccac gccggcatgc
     2281 cgcgcgactg gctggaatcg ggccgcgtcg agctcagcaa gtaccagtac gacgcgctgc
     2341 gcgaagccga cgcgatgatc ctggtcaccg aatggaaacg cttccgccag cccgatttcg
     2401 acgcgatgcg cgacctgctg ctcacgccgg tcatcttcga tggccgcaac cagtacgacc
     2461 cggtgctgat gagcgattac ggcttcacct attacggcat cgggcgcagc aatgtggcgg
     2521 cggggtgatg catcgaattt cttggtcgcg ggcatggccc gctcctacgg caccggcacc
     2581 ggcctcccta acccgtagga gcgggccatg cccgcgatcg gatgttgttg atcatcaatc
     2641 gcgggcatgg cccgctcctg catcgaccaa ttacctcatg agagattcga acccatgttg
     2701 attcctgttc tactctccgg tggcgtcggc agccgcctct ggccggtctc ccgcgagctc
     2761 tatcccaagc agttcctgcc gctcgccgat gagaagctct cgctgttcca gcagacgctg
     2821 gagcgcctcg gcggcatgcc ggcgctgggc gagcccgtcg tggtgtgcaa cgaggaacac
     2881 cgcttcctgg tggcgcagca gttgcgtgac atggggcacg gtggcgcctc gatcatcctg
     2941 gagcccatcg ggcgcaatac ggcgccggcc atcgcgctgg ccgcgatcga tgcgctgcgc
     3001 cagaccgagg acgccgtgct gctggtgctt cctgccgatc acgtgatccg cgactgcgac
     3061 gcgctgcagc gcgcggcgca gaccggcatg gcgctggccg gtgccggcca cctggtcacc
     3121 ttcggcatcg tgccgcaagc ccccgaaacc ggctacggct acatcaggcg cggcgccgcg
     3181 ctggacgatc aggtctacgc ggtcagccat ttcgccgaaa agccggacct cgagaccgcc
     3241 aacgcctacg tcgcaagcgg tgaatacttc tggaacagcg gcatgttcct gtttcgcgca
     3301 gaccgttacc tggaggagct cgacaagtac gccccgcaga tgctggccgc ctgccgcaag
     3361 gcctacgagc aggccagcga cgacctcgac ttcatccgcg tgcacaagga gtcgttcacc
     3421 gcctgcccca gcgactccat cgactacgcc gtgatggagc gcaccgaccg tgccgcgatg
     3481 gtgccgctcg attgcggctg gaacgacgtg ggctcctggg gagcgctgtg gcagaccggc
     3541 acgccggacg cgcgcggcaa cgtgagccgc ggcgacgtga tcctgcgcaa cgtctcgaat
     3601 gcctacgtgc gcgccgaatc gcggctggtc gcggccgtgg ggctggagga cgtggtgatc
     3661 gtggaaaccg ccgacgcggt actggtcgcc aacaaggatt cctcgcagga cgtgaagcac
     3721 atcgtggcgc acctcaagct cgagggtcgc gaggaagcgc tgctgcacac gcgcgtctat
     3781 cggccgtggg gctcctacga gagcctcgtc gagtccgagc gcttccaggt caagcgcatc
     3841 atcgtcaatc cgggccagca gctgtcgctt cagatgcacc accaccgcgc cgagcactgg
     3901 gtggtggtga agggcacggc gcgcgtgacc catggcgagc aggaaacgat gctcggcgag
     3961 gaccagtcga cctatatccc gctcggcacc aaacaccgcc ttgccaaccc cggcgtgatc
     4021 ccgctggaga tcatcgaggt gcagaccggc agttacctcg gcgaagacga tatcgtgcgt
     4081 ttcaacgacg tctacgggcg ggccggttcc tgatgccggc gcatgccctc ctgtaggagc
     4141 gggccatgcc cgcgaccgat gaacgacaac atccaatcgc gggcatggcc cgctcctaca
     4201 tggggaatga atcgtcccca tgatccgcct cctcgccagc ctgttcacgc tcgccctcgt
     4261 ggccctgctg ggcatggccg tgctgctgtt ctccgccatc gacaccaggc cgctggtcga
     4321 ccgcggcgaa accatctcca ccaacgccat cgaccaggcc cctaagctgc tcctcgccaa
     4381 cgacccccgc cgcctgcgtc cgggagagga gcgctcggcg ctgatcccgg caaccctgct
     4441 cgacgaaggc gtcaattatg tcgccacgca gcttctgcac tgtcgcggcg cgctgtcgct
     4501 gcaagatgac tcggcagaaa tacgtctcag cctgccgctt ccgggcacgc ccggctacct
     4561 gaacctgcgc gccacggtgc ccgaagccgc gggcgagccg cggatcgcgg cgctgcgggt
     4621 gggtgatgta cagattcctg ccgtcttcgc ggaccaggcc ctggatctcg cgctgaattc
     4681 cctcggttac gggcagcaat ggcgcatggc gcgcgccgcg gtgcgcgaac tcgccttcga
     4741 cccggcgcgc ggcatggtcg atgtgcgcta tgcctgggat ccggcgctgc tggatagcgc
     4801 ccgcgccgcc gcgctcgatc ccgcggacgt ggatcgcatc cgcgaggcgc aggcggcctt
     4861 cgcgacgctg ctcgaaggga aaccaagggg cgagcgagtc actctcgccg aagtcctggc
     4921 gccattgatg tccggcaacg ggcagggccg cgagcaacgc cgcgcggcgc tgctcgtgct
     4981 cgcgagctcg ctcgctgggg ggaagctggc gaacatcgtc cccgaggcag cctcctggcc
     5041 gcagccgcca cgggttgcca tcacgctgcg cggccggctc gacagcgccc agcatttcgt
     5101 gatctcggcc gcgctggccg cctgggccgg tgaacccgtt gccaccgcca tcggtctcta
     5161 caaggaactg gaggacgcgc gccgcggcag cggcttctcg ttcgcggatc tggccgccga
     5221 ccgcgccggc acccgcttcg gcgaactggt cgccgctaat gcggcgcgcc tcgacgaggt
     5281 gctggccggc acgcccggtg acgccgagct gctcccgccg ctggacggcc tgcccgaatt
     5341 tctcccggaa gccgagttcc gccgccgcta cggcggcccc ggcgagcccg cctacgagaa
     5401 agtcgcggac gagatcgagc ggcggctgtc gaggctggcg ctataccggt gagggaatgg
     5461 acctgccgct ggccccggat tcgttgtcat cgtttatgcc aatcggctaa cgtgatccat
     5521 acttgctacc gttgtgtcgc gggcatggcc cgctcctaca cggttgggcg gtttttgggt
     5581 cgcgggcatg gcccgctcct acgtcactgg cgctggctct cctgtgggtc gccattcatg
     5641 gcgacattgt ccgcatgaat gcggacccac gggccgtagg agcgggccat gcccgcgacc
     5701 aatgtgttcg cggcttggca ccatgcgcgc gatcaatgtt tcgcgaattc acgtcgtgat
     5761 caaatagaaa acgtttatga acaaaccaac ccgggctacc aaatcaatga tcaagaaatg
     5821 cctcttcccc gtcgccggct atggcacgcg cttcctgccg gccaccaagt ccatgcccaa
     5881 ggaaatgctg cccatcgtca acaagccgct ggtgcaatac ggggtagagg aggcgctcga
     5941 cgccggtctg ttcgagatcg ccttcgtcac cggccgcggc aagcgcacga tcaacgacca
     6001 cttcgacatc agctacgaac tggagcacca gatcgccggc accgacaagg agcagaccct
     6061 ggtcggcatc cgcaacgtca tcgaccgcgc ggtgttctcc accacgcgtc aacgcgagat
     6121 gaagggcctg ggtcacgcga tcctcaccgg ccagacgctg atcggcgacg agcccttcgg
     6181 cgtgatcctc gccgacgacc tctgcttcaa cgagggcggc gacggcgtga tggcgcagat
     6241 ggtcaagatc tacaagcagg tgcgctgcag catcgtggc
//
LOCUS       DAORUQ010000174         6276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14635_length_6276_cov_6.713551, whole genome shotgun sequence.
ACCESSION   DAORUQ010000174 DAORUQ010000000
VERSION     DAORUQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_6276_cov_6.713551"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLW13_13040"
     CDS             complement(<1..686)
                     /locus_tag="PLW13_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71352.1"
                     /translation="MKNAFASLLLLCCVLVARAAAAREPWEYAWTETRSPHFVVLSAL
                     GSGRGAELAQSLEDFRFAAKIVTNTGRFDERVPTYVYLVPRTDTDLGFGRYVLGYLRP
                     TMRANYAVLAPDRQLGTDIVLKHEYAHFLMHNRDHTIYPAWYEEGFAELVSSLELEGD
                     AIALGKPVPRRVQALAAGDWVGFARLISLRHTADLHGMDQTMFYAQSWLLTHYLSLGR
                     EGRDFAADTQR"
     gene            749..1603
                     /locus_tag="PLW13_13045"
     CDS             749..1603
                     /locus_tag="PLW13_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY71353.1"
                     /translation="MTNLGYDATSRVLETAKGRIHYHEAGSGPVLLMIHGSGPGVSGW
                     ANFQGNLPVFARHFRCVFIDLPGYGGSDPVEGAPVPACVDAVLRLMDGLGIERAHILG
                     NSLGGIVGSFVAAYHPQRVQRFVTIGGIGMNLFTAFPGEGLRLLTAFAEEPTRENIET
                     WLRAMVFDQSIITDELIESRLKAATDPVTLATSRQLYSRASADALAAEFRGPNATQRV
                     AHLAAIQAPTLITWGRDDRVSPLDMALLPMRVIPHAELHVFPNCGHWAMIECKEAFET
                     LVTGFLSL"
     gene            complement(1750..2622)
                     /locus_tag="PLW13_13050"
     CDS             complement(1750..2622)
                     /locus_tag="PLW13_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71354.1"
                     /translation="MSEENRPVAMVTGASRGAGKGIALALGGRGYTVYVTGRSQRAGD
                     APLPGTIHETAAAIDRLGGHGIAVSCDHGDDEQVRALFERVAREQDGRLDILVNNACA
                     IPDELVQPGAFWEKPLSLLSLLNVGMRSNYVASYYAAPLMVARRCGLIVHTSSFGGTC
                     YMHGPAYGAGKAAVDKMAHDMGVELRPHNVAVVSIWMGLLKTERTDVALSDPELARHY
                     EHLLETMESPQLTGLVIDALARDAHLMDRSGRIWIGAELAQQYGIHDVDGRQPPSHRP
                     MLGDPTVFSAAVIA"
     gene            complement(2644..3042)
                     /locus_tag="PLW13_13055"
     CDS             complement(2644..3042)
                     /locus_tag="PLW13_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HQY71355.1"
                     /translation="MGSDNTHSKIIGYLLWIFGFMGAHRFYFGKPVSGTIWFLTLGLL
                     GIGWIIDLFLIPGMHRDAERRFREGPIDYDLAWILLTFLGIFGVHRFYMGKWLSGLLY
                     LFTGGVFLLGVLYDYWTLNRQIDELAGQAG"
     gene            3130..5823
                     /locus_tag="PLW13_13060"
     CDS             3130..5823
                     /locus_tag="PLW13_13060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HQY71356.1"
                     /translation="MTIRNLDKLFSPASVAVIGASERAGSIGSVLTRNLVTGGFAGPV
                     WGVNPHYDTTVHGAPCFPSVAALPAAPDLAIIVTPMASAPGLVAELGARGTRAAVVIS
                     ASREDAEGAAIKTAILQAARPYTMRIVGPNCLGVMVPATGLNASFAHLPPIRGRLAFV
                     TQSGAIVTSMIDWAQSRDIGFSHVVSLGDMSDVDIGDMLDYLANDGGTDAILLYIEGI
                     TGARKFVSAARAAARTKPVIVVKGGRFAAGARAAATHTGAMMGRDEVYDAVFRRTGMV
                     RVYTLQELFNAAETLSRVHRLRGERLAILTNGGGLAVLAADALASLEGTLATLGENTL
                     AALDALLPPNWSRANPVDVIGDASPARFAEAARILAADKGIDALMIIHCPTAVCPGPE
                     AARAVIEALAGRQAPQLLTSWVGGATALAARELFAAAHVPTYPTPEQAIRGFMHAVNH
                     ARGQRILLETPELAPEAREPELRAVADILAQARAAGRPWLDSAQTRAVLKAYGFAVIE
                     VEQVATPAQAGAAAARIGAAVALKIVSPDIPHKTDFGGVVLDLIGPEATRAAAEAMQA
                     SIRARLPEALIEGFSVERMVHRPGAYELILGMSPDPQFGPVLVFGHGGTATETIADTA
                     LALPPLNLHLARSLISRTRIARLLEGARGMPGADLDAIALALVNLSQLVCDFPGIAEL
                     DINPLLADAQGVIVLDARIRLGDAVAPGTDRLAIKPYPRELEEDVPLADGRVLWLRPV
                     LPEDEPALQRAFSKLTPDEIYLRFFAPLKALSHAMATRFTQIDYDRQMVLILTEHGAP
                     GTTEYYGSVNIVCDPDLQQAEYAILVRHDMTGKGLGGLLMRRIIAYARSRGIGEIFGE
                     VLAQNHSMLSLCQQLGFRLETNPHDQSVVTVRLPLDDARTD"
     gene            complement(6014..>6276)
                     /locus_tag="PLW13_13065"
     CDS             complement(6014..>6276)
                     /locus_tag="PLW13_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71357.1"
                     /translation="LDRHFREDPEAARLFYEGFEVLRVYYGDDNPNKAFLDGFPDFEM
                     FPHFFVVETDGRVLASVTADVLIADAKYQTALIRSFAKHWKK"
BASE COUNT         1030 a         2205 c         2091 g          950 t
ORIGIN      
        1 cgctgcgtat cggcggcgaa gtcgcgcccc tcgcggccca ggctcagata atgcgtgagc
       61 agccaggact gcgcgtagaa catcgtctga tccatgccgt gcagatcggc cgtgtggcgc
      121 agcgagatca ggcgggcgaa tccgacccag tcaccggcgg cgagtgcctg cacgcgcctc
      181 ggaacgggtt tgcccagcgc gatcgcatca ccctccagct ccaggctgga caccagctcg
      241 gcgaagccct cctcgtacca cgccggatag atcgtgtggt cacggttgtg catcaggaaa
      301 tgcgcgtact cgtgcttcag cacgatgtcg gtgcccagct gccggtccgg tgccagcacc
      361 gcataattgg cccgcatggt cggccgcaga taacccagca cgtaacgccc gaagccgagg
      421 tcggtatcgg tgcggggcac gaggtacacg taggtgggca cccgttcatc gaagcgcccg
      481 gtgttcgtga cgatcttcgc cgcgaagcgg aagtcctcga ggctctgcgc gagttcggcc
      541 ccgcgcccgc tgccgagcgc actcagcacc acgaagtgcg gcgaacgggt ctccgtccac
      601 gcatactccc acggttcgcg cgctgccgcg gcacgggcga cgagcacgca acacagcagc
      661 agcagtgatg cgaatgcgtt tttcattttt ttctccgcca gcgcatagaa tgcctggcgc
      721 acgatcgcat aaacaaggac agcaggacat gaccaatctg ggctacgacg ccaccagccg
      781 cgttctggaa accgccaagg gccgcatcca ctaccacgag gccggcagcg gccccgtgct
      841 gctgatgatc cacggctccg ggcccggcgt gagcggctgg gcgaatttcc agggcaacct
      901 gccggtgttc gcgcggcact ttcgctgcgt tttcatcgac ctgcccggct acggcggcag
      961 cgacccggtc gagggcgcgc cggtgccggc ttgcgtcgat gcggtgctgc gcctgatgga
     1021 cgggctcggc atcgagcgcg cgcacatcct cggcaattcg ctgggcggta tcgtcggttc
     1081 cttcgtggcc gcgtatcacc cgcagcgcgt gcagcgcttc gtcaccatcg gcggcatcgg
     1141 catgaacctg ttcacggcct tccccggcga ggggctgcgc ctgctcacgg ccttcgccga
     1201 ggagcccacg cgcgagaaca tcgagacctg gctgcgcgcg atggtcttcg accagtcgat
     1261 catcaccgat gagctgatcg agtcgcggct gaaggccgcc accgacccgg tgaccctcgc
     1321 gaccagccgc cagctctact cgcgcgcttc agccgacgcg ctggcggcgg agttccgcgg
     1381 cccgaacgcg acacagcgcg tggcgcacct cgccgcgatc caggcgccca cgctgatcac
     1441 ctggggacgc gacgaccgcg tcagcccgct cgacatggca ctgctgccga tgcgcgtgat
     1501 cccgcacgcc gagctgcacg tttttccgaa ctgcgggcac tgggcgatga tcgagtgcaa
     1561 ggaagcgttc gagacgctgg tgaccggatt cctctctctc tgacgggatt ttttgttcgt
     1621 gggtcgggat tcatcccggc aagggttcgg cagcgccgga cccgccattt ccccgcaggc
     1681 agctgtcggc atgaatgccc atcaacacac gaatgccgac ccacggcact gtgtccgcat
     1741 gatgcggacc tacgcgatga ccgcggcact gaaaaccgtc gggtcgccga gcatcgggcg
     1801 gtgcgagggc ggctggcggc cgtcgacgtc gtggatgccg tactgctgcg ccagctccgc
     1861 gccgatccag atgcgcccgc tgcgatccat caggtgcgca tcgcgcgcca gcgcgtcgat
     1921 caccaggccc gtgagttgcg ggctctccat ggtttccagc aggtgctcgt aatggcgcgc
     1981 cagctcggga tccgacagcg ccacgtcggt gcgctcggtc ttcagcaggc ccatccagat
     2041 cgagacgacc gcgacgttgt gcggacgcag ctccacgccc atgtcgtgcg ccatcttgtc
     2101 gaccgcggcc ttgcccgccc cgtaggcggg cccgtgcatg tagcaggtgc cgccgaagga
     2161 ggaggtgtgc acgatcagcc cgcagcggcg cgccaccatc agcggggcgg catagtagct
     2221 cgcgacatag ttcgagcgca tgcccacgtt caacaggctg agcagcgaca acggcttctc
     2281 ccagaacgcg cccggctgca ccagttcgtc gggaatggcg caggcattgt tgaccaggat
     2341 gtcgaggcga ccgtcctgct cgcgcgcgac gcgctcgaac agcgcgcgca cctgttcgtc
     2401 gtcgccatga tcgcaggaca ccgcgatgcc atggccgccg aggcggtcga tcgcggcggc
     2461 ggtttcgtgg atcgtgccgg gcagcggcgc atcgcccgcg cgctgcgaac gcccggtgac
     2521 gtagaccgtg taaccacggc caccgagggc gagcgcgatg cccttgcccg caccgcggct
     2581 cgcgcccgtg accatcgcta ccgggcgatt ctcttcactc atcgggttct ctcctgtatg
     2641 gcttcagccg gcctggccgg ccagttcgtc gatctgccgg ttcagcgtcc agtagtcata
     2701 gagcacgccg agcaggaaca cgccgcccgt gaacaggtac agcagcccgc tcagccactt
     2761 gcccatgtag aagcggtgca cgccgaagat cccgaggaag gtcagcagga tccacgccag
     2821 gtcgtagtcg atcgggccct cgcgaaagcg ccgctcggca tcgcggtgca tgcccgggat
     2881 caggaacagg tcgatgatcc agccgatgcc cagcaggccc agcgtcagga accagatcgt
     2941 gcccgagacc ggcttgccga agtagaaacg gtgcgcgccc atgaaaccga atatccacag
     3001 caggtagccg atgatcttcg aatgcgtgtt gtcgctgccc atcacgagcc tccggatgcg
     3061 ggaaggacat agtgtaacca atccgtgttt caatcagcga acccgaaacg caccggccgg
     3121 atctcgacga tgaccatacg caacctcgac aagctgttct cgcccgcctc ggtcgccgtg
     3181 atcggcgcga gcgaacgcgc cggcagcatc ggcagcgtgc tcacccgcaa cctggtgaca
     3241 ggcggcttcg ccggcccggt gtggggcgtc aacccgcatt acgacacgac cgtgcacggc
     3301 gcgccctgct tcccctcggt ggcggcgctg cccgccgcgc cggatctcgc gatcatcgtg
     3361 accccgatgg cctccgcccc cgggctggtc gcggaactgg gcgcgcgcgg cacgcgcgcc
     3421 gcggtcgtga tcagcgcgag ccgcgaggat gccgagggcg ccgcgatcaa gaccgcgata
     3481 ctccaggccg cgcgccccta caccatgcgc atcgtggggc ccaactgcct cggcgtgatg
     3541 gtgccggcaa caggactgaa cgccagcttc gcgcacctgc cgccgatcag ggggcggctc
     3601 gccttcgtca cgcagtccgg cgcgatcgtg acctcgatga tcgactgggc gcaatcgcgc
     3661 gacatcggct tctcgcacgt cgtctcgctc ggcgacatga gcgacgtgga catcggcgac
     3721 atgctcgatt acctcgccaa cgacggcggc accgacgcca tcctgctcta catcgagggg
     3781 atcacgggcg cgcgcaaatt cgtttccgcc gcgcgcgcgg ccgcgcgcac caagcccgtg
     3841 atcgtggtga agggcgggcg cttcgccgcc ggcgcgcgcg ccgcggccac gcacaccggc
     3901 gcgatgatgg gacgcgacga ggtctacgac gcggtattcc gccgcaccgg catggtgcgc
     3961 gtgtacacgc tgcaggagct cttcaacgcc gccgagacgc tgagccgcgt gcaccggctg
     4021 cgcggcgaac gcctcgcgat actcaccaac gggggcggcc tcgcggtact cgctgccgac
     4081 gcgctggctt cgctggaagg cacactggca acgctgggcg agaacaccct cgccgcgctg
     4141 gacgcgttgc tgccgccgaa ctggtcgcgt gccaacccgg tcgacgtgat cggcgacgcc
     4201 tcgcccgcgc gtttcgccga ggcggcgagg atcctcgccg ccgacaaggg catcgacgcc
     4261 ctgatgatca tccactgccc gaccgccgtg tgcccgggcc cggaggcggc acgcgccgtg
     4321 atcgaggcat tggccggcag gcaagcgccg cagctgctca cgagctgggt gggtggcgcg
     4381 accgcgctgg ccgcgcgcga gctttttgcc gccgcgcacg tgccgaccta tcccacgccc
     4441 gaacaggcca tccgcggctt catgcacgcg gtgaaccacg cccgcggaca gcgcatcctg
     4501 ctggagaccc cggagctcgc gcccgaggcg cgcgaaccgg aactgcgcgc ggtggcggac
     4561 atcctggcgc aggcgcgcgc cgccgggcgg ccgtggctcg acagcgcgca aacccgcgcg
     4621 gtgctgaagg cctacgggtt cgcggtgatc gaagtcgagc aggtcgccac acccgcgcag
     4681 gccggcgcgg cggcggcgcg catcggcgcc gcggtggcgc tgaagatcgt ctcgcccgac
     4741 atcccgcaca agaccgattt cggcggcgtc gtgctcgacc tgatcggccc cgaggccacg
     4801 cgtgccgcgg ccgaggcgat gcaagcgagc atccgcgcgc gcctgcccga agcgctcatc
     4861 gaaggcttca gcgtcgagcg catggtgcac cgccccggcg cctacgagct gatcctcggc
     4921 atgagcccgg acccgcagtt cggcccggtg ctcgtcttcg gccacggcgg caccgcgacc
     4981 gagaccatcg ccgacaccgc gctggcgctg ccgccgctga acctgcacct ggcgcgctcg
     5041 ctgatctcgc gcacgcgtat cgcgcgcctg ctggagggtg cgcgcggcat gcccggcgcc
     5101 gacctcgacg cgatcgcgct ggcgctggtc aacctgtcac agctggtgtg tgatttcccc
     5161 gggatcgccg agctcgacat caatccgctg ctcgccgacg cgcagggcgt gatcgtgctc
     5221 gatgcgcgca tccgcctcgg cgatgccgtg gcgccgggaa ccgaccggct cgccatcaag
     5281 ccctacccgc gcgaactgga ggaggacgtg ccgctcgccg acgggcgcgt gctgtggctg
     5341 cgcccggtgc tgccggagga cgagccggcg ctgcagcgcg ccttcagcaa gctgacgccg
     5401 gacgagatct acctgcgttt cttcgcgcca ttgaaggccc tgtcgcacgc gatggccacg
     5461 cgcttcacgc agatcgacta cgaccgccag atggtgctga tactgacgga gcacggcgcg
     5521 ccgggaacca cggagtacta cggctcggtg aacatcgtct gcgacccgga cctgcagcag
     5581 gccgagtacg cgatcctggt tcgccacgac atgaccggca aggggctcgg cggcctgctg
     5641 atgcgccgca tcatcgccta cgcgcgcagc cgcggcatcg gcgagatttt cggcgaagtg
     5701 ctggcccaga accacagcat gctcagcctg tgccagcagc tgggattccg cctagagacg
     5761 aacccgcacg accagagcgt ggtgacggtc aggctgccgc tggatgacgc caggacggac
     5821 tgaacggcgg caaactgaac gctgggtacc accacgacag ccagtgccac gctggctgtc
     5881 gtgcgagggc cgtctgcggc atggatgccg cagtcgagac tacagggatg tattcacggc
     5941 gtgtccgagc aggatagcca gcgtggcact ggcgggccgg aaatacctgc gttgtcgcgg
     6001 gcgtggcccg ctcctacttc ttccagtgct tcgcaaacga acggatcagc gcggtctggt
     6061 atttcgcgtc ggcgatcagc acgtcggcgg tcaccgaggc gagcacgcgg ccgtcggtct
     6121 ccaccacgaa gaaatgcggg aacatctcga agtccgggaa accgtcgagg aaggccttgt
     6181 tgggattgtc gtcgccgtaa tagacgcgca gcacctcgaa gccctcgtag aacaggcgcg
     6241 ccgcttcggg atcctcgcgg aaatggcggt ccagca
//
LOCUS       DAORUQ010000175         6237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14786_length_6237_cov_7.073277, whole genome shotgun sequence.
ACCESSION   DAORUQ010000175 DAORUQ010000000
VERSION     DAORUQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14786_length_6237_cov_7.073277"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLW13_13070"
     CDS             <1..736
                     /locus_tag="PLW13_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQY71358.1"
                     /translation="IGPDLERLGKLERQRGMGTWISDHTEADLPNVFAALGRSQRFVR
                     CNPPHACLAQEIERLVACGAQVIMLPHFHSVGEAGHFVRAVDGRARTVLLVETVSAAS
                     RVGQLCRIEGLDEIHFGLNDLSLDLGVKNHFAVLCSTLLEDACATLAAVAFPFAVGGI
                     GRALDATLPVPSELVYAQYPRLGATGALVSRVFFKDLPEEQFAAAVTRARERLTYWSQ
                     QSAGRLREAREELDALVVAGMSRAGR"
     gene            733..2724
                     /locus_tag="PLW13_13075"
     CDS             733..2724
                     /locus_tag="PLW13_13075"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY71359.1"
                     /translation="MSGAAPPPGAVSRWSACFQAGRLDECQRLAQELVRDCPDSGKAW
                     QLLGMSRFARGDLRQALAHLRRAAAIEGGDRSIWDNLGIVLHKSGDHAGARAAFTTAL
                     ALAPAAAGIWSNAAGNELDAGNAAEAQRLAARAVALAPQLATGWLQLGNALSRLQRSV
                     EAEAALREALRIQPRHPDALISLGTTLAAQNRLREACAAAEAALRIDPGLSRAHVNLG
                     SIHDRLGDTRTARDHYRKARELDPADLGSWSSELYCLSHDASLDAQQVFDAHKAFGEQ
                     AEARFGTQPAPHANDRDPARRLRVGVVSGDFRDHPVARFLEPVWRERDRAQLQLLAYD
                     TNPAADAMAQRLRALADAWADVSTLSDEALAARIRADGIDILIDHSGHTAGNRLGVFA
                     RKAAPVQVMWVGYPGTSGLRAMDYRLVDPVLAPPGRFDHLFIERLAYLPVMLVFGKPE
                     RLPALTPPPLARNGYITYGSFNRMNKLGDAVIALWAEILRRTPGSRLVLGAVTDDAVA
                     EALRGRFAARGIAHGRIAFLPRLDMAGYLAAHERIDLLLDAFPWASSTTAQFGLWMGV
                     PTLTLAGDSLVARLGTAAMSAAGLDRFVAGSAPQYVEIAVRTAAQPDELQRLRYSLRN
                     RLEEDRHRVPAQVARALEVRLRQMWQRWCAGLAPCVLDS"
     gene            2742..3257
                     /locus_tag="PLW13_13080"
     CDS             2742..3257
                     /locus_tag="PLW13_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HQY71360.1"
                     /translation="MGNDGIKDTQTVAADGRMLRELIDGVHLHEMQNIITRNGTTTEV
                     VRRDWDIGRLAINHMIHVTLRAHAISAWHSHHLQTDRIFVTDGTLRIVLFDDREGSPT
                     KGKLDVLHLGRMRPATLLIPPFVWHGIQNLENQASSFVNFFDRAYCYENPDEWRLPTD
                     TDAIPYRFAPD"
     gene            complement(3282..4814)
                     /locus_tag="PLW13_13085"
     CDS             complement(3282..4814)
                     /locus_tag="PLW13_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HQY71361.1"
                     /translation="MNKGIVVGAVLALAGFGAGMYFAKQDQPLPAATTGSAGASYAGG
                     FKAEGEAAEAVKAGVASGNIKAVAGPAGEAVIVVKDGESIQEAVKRAAPGTTIQVLPG
                     TYHETVYIDKDGIRLIGVIEEGRRAVLDGQGKLNDAILYSGNNVVVENLAITKYKGNG
                     IMSQAGNNFEIRNNLIVDTGVYGIFPQLGQNGVVEHNVVSGIEDAAIYVGMSDNIHVA
                     HNDVFDSVAGIEIENSRHAIVENNYVHNNTGGILAFVTPGLPIKTTFDVIIRHNFIVG
                     NNHDNFGAPGSTVAGIPPGTGVLIMAADDVVIEGNIISDNKTAGILVTDHANAANITV
                     DPESEPNSDRVKILDNTMLDNGYDTIPEVKALMLTELQRGNPDIVRVGPSPGSCIRNR
                     GRYVTVGVKDFAECDFSNTAATETYLLPPVPARVIAPDERGKIAYLGVCSGCHIYTGR
                     MIGPPVQVIQALYMDNPQGLADYIANPVKKRDDYPEMPPQNYLDAATRLAVAEYMLQV
                     KN"
     gene            complement(4832..6061)
                     /locus_tag="PLW13_13090"
     CDS             complement(4832..6061)
                     /locus_tag="PLW13_13090"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQY71362.1"
                     /translation="MGVGDEKRGDDIVLLRRHAGKALAATALAAPAEEFRLREHCPPS
                     FEKTAAGRCELRSLYQFYDSLEGKGVGGTQTGLPPQRDGFSPQQIDLGRYLFFDPLLS
                     GEGKMSCASCHHPDLGFSDGRARSLGPHGEDVGRAAPTLWNVAFLKTFFWDARAHSLE
                     EQATGPLYSDKEMGNTPERLMSTLGGNAIYARLFREAFPRDSEAVTLDQVYTALAAFQ
                     SSLISLNSRYDRYAQGYHEALSPREIEGMNVFRSFVARCAECHTPPLFTNQQVAVLGT
                     PEPEGRPLDVGAEKTFAAPKLKGAFKVPTLRNVAQTAPYMHSGRFATLRETVAFYTGG
                     RGHAIPPGVAMQPHWHIWDPKLDDEELDRLVDFLQALSDETFSPEVPVAVPSGLPPVG
                     NSPLAKSPAVAHAARTR"
     gene            6008..>6237
                     /locus_tag="PLW13_13095"
     CDS             6008..>6237
                     /locus_tag="PLW13_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQY71363.1"
                     /translation="MATKEDDVVTTLFVANTHTQLLFFTTDGMVYKLKTWRLPLAGRT
                     AKGKAIIIILPIPQGVSIAAIMPVDVPEEEWET"
BASE COUNT          930 a         2160 c         2125 g         1022 t
ORIGIN      
        1 tatcggccca gacctggaac gcctgggcaa gctggaacgc cagcgcggga tgggcacctg
       61 gatttccgac cacaccgagg cggacctccc gaacgttttc gcggcgctag gccgctcgca
      121 gcgcttcgtg cgctgcaatc cgccccacgc ttgcctcgcg caggagatcg agcgcctggt
      181 cgcttgtggc gcccaggtca tcatgctgcc gcacttccat tcggtcggcg aagccgggca
      241 tttcgtgcgc gcggtcgatg gcagggcgag gacggtgctg ctggtcgaga ccgtgagcgc
      301 ggcatcccgg gtcgggcagc tgtgccggat cgagggcttg gacgagatcc acttcgggct
      361 gaacgacctg tcgctggacc tcggcgtgaa gaaccacttc gcggtgctct gcagcacgct
      421 gctcgaagac gcctgcgcga cactggcggc ggtcgctttc ccctttgccg taggcggcat
      481 cggccgcgcg ctggacgcga cgctccccgt tccctccgag ctcgtgtacg cgcaataccc
      541 gcgcctgggc gcgaccggcg cactggtgtc gcgcgtgttc ttcaaggacc tgccggaaga
      601 gcagttcgcc gcggcggtga cccgggcccg cgagcggctg acgtactggt cgcaacagtc
      661 cgccggcagg ctacgcgagg cccgcgagga actggatgca ctggtggtgg caggcatgag
      721 ccgggccggc cggtgagcgg cgccgcgccg ccgcccgggg ccgtgtcgcg ctggagcgcc
      781 tgcttccagg cgggccggct cgacgagtgc cagcggctgg cgcaggagct ggtccgggac
      841 tgccccgatt cgggcaaggc ctggcagctg ctcggcatga gccgcttcgc ccggggcgac
      901 ctgcgccagg cgctggcgca tctgcgccgc gccgcggcaa tcgagggcgg cgaccgctcg
      961 atctgggaca acctcggcat cgtgctgcac aaatccggcg atcatgccgg cgcacgcgcg
     1021 gcgttcacga cggcactggc gctcgcaccc gccgcggcgg gcatctggtc gaacgccgcg
     1081 ggcaacgagc tcgacgccgg caacgccgcc gaggcgcagc gactggcggc cagggcggtc
     1141 gccctcgcgc cgcaactcgc caccggctgg ctgcagctcg gcaacgcgct ctcgcggctg
     1201 cagcgcagcg tggaggccga ggccgcgctg cgcgaggcgc tgcgcatcca gccccgccat
     1261 cccgatgcgc tgatcagcct gggcacgacg ctggcggcgc agaaccgact gcgcgaagcc
     1321 tgcgcggcgg cggaggcggc gctgcggatc gaccccggcc tttcccgcgc gcacgtcaac
     1381 ctcggcagca ttcacgaccg actcggtgac actcggaccg cgcgcgatca ctaccgcaag
     1441 gcccgcgaac tggatccggc ggacctcggc agctggagca gcgagcttta ctgcctgtca
     1501 cacgacgcga gcctggacgc acaacaggtc ttcgacgccc acaaggcatt cggcgagcag
     1561 gccgaggccc gcttcggcac gcaaccggcg ccgcatgcga acgaccgtga tcccgcgcgc
     1621 cggctgcgcg tgggcgtcgt gtcgggcgat ttccgggatc atccggtggc gcgcttcctg
     1681 gagccggtgt ggcgcgaacg ggatcgcgcg cagctgcagc tcctcgccta cgacaccaac
     1741 ccggccgcgg acgccatggc gcagcgcctg cgcgcgctcg ccgacgcgtg ggccgacgtg
     1801 tcgacgctca gcgacgaggc gctcgcggcg cgcatccgcg ctgacggcat cgacatactc
     1861 atcgaccatt ccgggcacac ggccgggaac cgcctcggtg tcttcgcccg caaggccgcg
     1921 ccggtgcagg tgatgtgggt cggctatccc gggaccagcg gcctgcgcgc gatggattac
     1981 cggttggtcg atccggtgct ggcgccgccc gggcgcttcg accacctgtt catcgagcgc
     2041 ctcgcctacc tgcccgtcat gctcgtcttc ggcaagcccg agcggttgcc ggcgctgacg
     2101 ccgccaccgc tcgcgcgcaa cggctacatc acctacggca gcttcaaccg gatgaacaag
     2161 ctcggcgacg cggtgatcgc gctgtgggct gaaatcctcc ggcgcactcc cggatcacgg
     2221 ctcgtgctgg gcgccgtgac cgacgatgca gtcgccgagg cgctgcgcgg gcgcttcgcg
     2281 gcccgcggca tcgcgcacgg gcgcatcgcc ttcctgccgc gcctcgacat ggccggctac
     2341 ctcgcggcgc acgaacggat cgacctgctg ctggatgcct ttccgtgggc cagttcgacg
     2401 acggcccagt tcgggctgtg gatgggtgtg cccacgctga cgctggcagg cgattcgctg
     2461 gtggcgcgcc tcggcactgc cgccatgtcg gcagccgggc tggaccggtt cgtggccgga
     2521 tcggcgccac agtatgttga aattgcagtg cgcacggcgg cgcagcccga cgagctgcag
     2581 cgcctgcgct actcgctcag gaaccggctc gaggaagacc gccaccgcgt cccggcgcag
     2641 gtagcgaggg cgctggaagt ccgactgcgc cagatgtggc agcgttggtg cgccggactt
     2701 gctccgtgcg tgctggatag ctaaggaaac caggaggcct catgggcaac gacggcatca
     2761 aggacacgca aacggtggca gccgacggcc gcatgctgcg cgagctgatc gacggtgtgc
     2821 atctccacga gatgcagaac atcatcacgc gcaacggcac caccaccgag gtggtgcgtc
     2881 gcgactggga catcggccgc ctcgcgatca accacatgat ccacgtcacg ctgcgtgcgc
     2941 acgccatcag cgcgtggcac agtcaccacc tgcagaccga caggatcttc gtcaccgacg
     3001 ggacgttgcg catcgtgctg ttcgacgacc gggagggctc gccgacgaag ggcaagctgg
     3061 acgtgctgca cctgggccgc atgcgccccg cgacgctgct gatcccgccc ttcgtctggc
     3121 acgggatcca gaacctcgag aaccaggcca gcagcttcgt caatttcttc gatcgcgcct
     3181 attgctacga gaacccggac gagtggcggc tgccgacgga caccgacgcc attccctacc
     3241 ggttcgcgcc cgactgaacg ggcgcggcgc ccggtgcggg atcagttctt cacctgcagc
     3301 atgtactcgg ccaccgcgag gcgtgtcgcc gcgtcgaggt agttctgcgg cggcatctcg
     3361 ggatagtcgt cgcgcttctt caccgggttg gcgatgtagt ccgcgagccc ctgcggattg
     3421 tccatgtaca gcgcctgtat cacctgcacc ggcgggccga tcatgcgccc ggtatagatg
     3481 tggcagccac tgcagacgcc gaggtaggcg atcttgccgc gctcgtcggg cgcgatcacg
     3541 cgcgccggca ccggcggcag caggtaggtt tcggtcgccg cggtgttgct gaagtcgcat
     3601 tcggcgaaat ccttcacgcc gacggtcacg tagcgcccgc ggttgcggat gcagctgccg
     3661 gggctgggac cgacgcgcac gatgtcggga ttgcctcgct gcagttcggt cagcatcagc
     3721 gccttcacct cggggatggt gtcgtagccg ttgtcgagca tcgtgttgtc gaggatcttc
     3781 acgcggtcgg agttcggctc cgactccggg tcgaccgtga tgttcgcggc gttcgcgtga
     3841 tcggtcacga ggatgcccgc ggtcttgttg tccgagatga tgttgccctc gatcaccacg
     3901 tcgtcggcgg ccatgatcag cacaccggtg cccggcggga tacccgcaac cgtggagccc
     3961 ggcgcgccga agttgtcgtg gttgttcccg acgatgaagt tgtgccggat gatcacgtcg
     4021 aaggtggtct tgatcggcag ccccggggtc acgaaggcga gtatcccgcc ggtgttgttg
     4081 tgcacgtagt tgttctcgac gatcgcgtga cggctgttct cgatctcgat gccggccacg
     4141 ctgtcgaaga cgtcgttgtg cgccacgtgg atgttgtcgc tcatgccgac gtagatcgcc
     4201 gcgtcctcga ttcccgagac cacgttgtgc tcgaccaccc cgttctgccc cagctgcggg
     4261 aagatgccgt agacgccggt gtcgacgatc aggttgttgc ggatctcgaa gttgttgccg
     4321 gcctggctca tgatgccgtt gcccttgtac ttcgtgatcg cgaggttctc caccaccacg
     4381 ttgttgccgg agtacaggat cgcgtcgttc agcttgccct ggccgtccag aaccgcgcgc
     4441 cggccttcct cgatcacgcc gatcaggcgg atgccgtcct tgtcgatgta gacggtctcg
     4501 tgataggttc ccggcaggac ctggatcgtg gtgccgggcg cggcgcgctt caccgcttcc
     4561 tgtatcgatt cgccgtcctt caccacgatc acggcctcgc ccgccggccc cgccaccgcc
     4621 ttgatgttgc cgctcgcgac accggccttc accgcctcgg ccgcttcgcc ctcggccttg
     4681 aagccaccgg cgtagctcgc tcccgcgctg ccggtggtcg cggccggcaa cggttggtcc
     4741 tgcttcgcga aatacatccc ggcgccgaaa ccggccagcg ccagcacggc gcccacgacg
     4801 atgcccttgt tcatcaggag tctccctgtg gttatctggt tcgcgcggcg tgtgccacgg
     4861 cgggggattt ggccagcggc gagttgccga ccggcggcag cccggagggc accgcaacgg
     4921 gcacctcggg gctgaaggtc tcgtcgctga gcgcctgcag gaaatcgacc aggcggtcga
     4981 gctcctcgtc gtccagtttc ggatcccaga tatgccagtg cggctgcatc gccacgccgg
     5041 gcgggatcgc gtggccgcgc ccgccggtat agaaagccac cgtctcgcgc aacgtggcaa
     5101 agcggcccga gtgcatgtac ggcgccgtct gcgcgacatt gcgcagcgtg ggcaccttga
     5161 acgcaccctt gagcttcggt gcagcgaagg ttttctcggc gcccacgtcg agcgggcgac
     5221 cctcgggctc cggggtgccg agcaccgcga cctgctggtt ggtgaacagc ggcggcgtgt
     5281 ggcattcggc gcagcgcgcc acgaaggagc ggaacacgtt catgccctcg atctcgcgcg
     5341 ggctcagcgc ctcgtggtat ccctgcgcgt agcgatcgta gcggctgttg agcgagatca
     5401 gcgaactctg gaacgccgcg agcgccgtat agacctgatc gagtgtcacc gcctcgctgt
     5461 cgcgcggaaa agcctcgcgg aacagccgcg cgtaaatcgc gttgccgccc agcgtcgaca
     5521 tcaggcgctc gggcgtgttg cccatttcct tgtccgaata cagcggcccc gtcgcctgct
     5581 cctccagcga gtgcgcgcgc gcatcccaga aaaaagtctt caggaaggcg acgttccaca
     5641 gcgtgggcgc ggcgcggccc acgtcctcgc cgtgcggacc gagcgaacgg gcgcgcccgt
     5701 cgctgaagcc cagatccggg tggtggcagc tggcgcagga catcttcccc tcgccggaga
     5761 gcaacgggtc gaagaacagg tagcggccga ggtcgatctg ctgcggactg aaaccgtcgc
     5821 gctgcggcgg caacccggtc tgcgtgccgc ccacgccctt gccctcgagg gagtcgtaga
     5881 actggtacag cgagcgcagc tcgcagcgcc cggccgcggt cttctcgaag ctcggcgggc
     5941 agtgctcgcg cagccggaat tcctccgcgg gcgcagccag cgccgtggcg gcaagggcct
     6001 ttccggcatg gcgacgaagg aggacgatgt cgtcaccacg cttttcgtcg ccaacaccca
     6061 cacccagctt ctgttcttca ccaccgacgg catggtctac aagctcaaga cctggcggct
     6121 gccgctggcc ggccgcaccg ccaagggcaa ggccatcatc atcatcctgc ccatcccgca
     6181 gggcgtctcc atcgccgcga tcatgccggt ggacgtgccc gaggaggaat gggaaac
//
LOCUS       DAORUQ010000176         6186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_14985_length_6186_cov_5.850922, whole genome shotgun sequence.
ACCESSION   DAORUQ010000176 DAORUQ010000000
VERSION     DAORUQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14985_length_6186_cov_5.850922"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PLW13_13100"
     CDS             complement(<1..170)
                     /locus_tag="PLW13_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY71364.1"
                     /translation="MNPAPRVAIIGAGIAGLSCATHLVQHGCTVSLFDRSRGPAGRMS
                     TRRGEGWQCDHGA"
     gene            complement(283..2151)
                     /locus_tag="PLW13_13105"
     CDS             complement(283..2151)
                     /locus_tag="PLW13_13105"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY71365.1"
                     /translation="MNRNTARLAALALACAVATETSAAEQSSYSPYAGRSFPERLLWG
                     DTHLHTNMSADAGSFGNRDVGPQDAYRFARGETVTAHNGMALRLARPLDFLVVADHSE
                     YLGLFPHLRAGDPNLLATETGRRWADIIAKGGRANAQMLMEWGAANFANKDVIGSAEF
                     RSSTWQEVVTNAERFNEPGRFTAIVGYEWTSMPNGDNLHRVVVFADGADKAGKTVPFS
                     SIDSSDPEKLWAYLADYEQQTGGQALAIPHNSNVSGGLMFAPRDFNGAAFDRDYAERR
                     SRWEPVMEVTQYKGDSETHPLNSPEDEFAGYEIWDRINLGGMTPQTPQMQPFQYARSA
                     LRIGLQQEAALGANPFKFGLIGSTDAHTGFAAAEENNFWGKSTKGEPAPGRWHESLFD
                     NPTVPELTFRESDMAASGYMAVWARENTRAAIFEAMKRREVYATTGPRIALRFFGGYG
                     FAAADASAPDLAATGYARGVPMGSDLPAASAGTAPTFLVAAARDALSGKLDRIQIVKG
                     WLDAKGETHERVYDVAWSGERKPDRKGRLPPVGNTVDAKNASWSNSIGAAELSTVWTD
                     PDFDPAERAFWYVRVIEIPTPRWTAYDRKFFGVEMPDSIPMSTQERAYSSPIWYTP"
     gene            complement(2175..2609)
                     /locus_tag="PLW13_13110"
     CDS             complement(2175..2609)
                     /locus_tag="PLW13_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY71366.1"
                     /translation="MSLRILVAVDGSEYSLRAVEHVLRLRDAHCDIEAHLLNVQMPLE
                     SGHVKQFIAREAIEEYYRDEGLAQLQAASAALEAAGQPCTTHIAVGHIAPTVTRYAAE
                     MHFDLIVIGTHGRTGLRHAVMGSVATEVIKLATVPVTVVRNA"
     gene            complement(2629..3369)
                     /locus_tag="PLW13_13115"
     CDS             complement(2629..3369)
                     /locus_tag="PLW13_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQY71367.1"
                     /translation="MPEFLFDPTFWLSVGQIILIDILLGGDNAVVIALACRKLPEEQR
                     RKGIFWGVAGAIGLRVVLIFFALQLLALPLLKVVGALLLLWIGVKLLTPEDEDGHANV
                     AGSSTLMGAIKTIIVADAVMSLDNVIAVAGAAKGSMALVVFGILISIPIIVWGSQFVL
                     RLMDRFPVVITLGAALLGWIAGEMLVTDVTVKPYLEGAPGWLHYASAAAGALFVVALG
                     TWLGRRRRPAGLEPIREIPLEASRDRNA"
     gene            complement(3463..3969)
                     /locus_tag="PLW13_13120"
     CDS             complement(3463..3969)
                     /locus_tag="PLW13_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQY71368.1"
                     /translation="MPGASPRQIDVRDIDALRAMLSENFGDWSEALVITQDMVNRFAE
                     LTGDRQWIHVDVERARRESPFGAPIVHGFLLLSLMPSLRAPPSFELLNVGSAANYGSG
                     GLRFLSPVTVGSSVHLRQRLKDVQAHRRGTLLTSEVAIHVVGQERPALLYDLQVLYVP
                     ASTPAAPA"
     gene            4065..4793
                     /locus_tag="PLW13_13125"
     CDS             4065..4793
                     /locus_tag="PLW13_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY71369.1"
                     /translation="MYRTLLVERRDRVAVITLDRPDQLNAYTVQMGEDLVHAFDALRA
                     DDSVHVAVLTGAGRAFCAGLDLNALRETPEPALPALGEERLIRSFGAELFNYPKPVIA
                     AINGAAAGIGVTMTLPCDIRIAAAGAKLAFPFAKLGIVPGLGSSFLLPRLLGPGQAKI
                     LALTGQTISAEEAQAIGLVDRVVAPESLLAETLELARAIAANSPQIVGLIKEALNAGA
                     TATSIDAALAFEHAQNARRSRILS"
     gene            complement(4835..6070)
                     /locus_tag="PLW13_13130"
     CDS             complement(4835..6070)
                     /locus_tag="PLW13_13130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY71370.1"
                     /translation="MTADATAPLAGVRVIDCSLLSPGATAAHLADLGAEVIKVEAPGG
                     DYVRSLTWPIIEGDSLLHHHVSRGKRSLTLDLRTESGVAVFRDLVRRADVVIEAMRPG
                     ALARRGLGYEALRLIRPGLVFCALSGYGMSGPYKDAPAHGVAFDSWAGLVKPGVDAEG
                     FCFIPEHPSIGMHAGPLIAALSICAALLRARATGTGAFLELGQSDAAAYMDWLRIETW
                     KAYERPQSEVTGNQTDDYVRRAPGTAGMEKGVRYQYYASRDGHILFMASEQEFWKNFC
                     TGVGRLDLFERWPGKRYGDHATGNRELQAILRDIFATRTTQDWMAFAVQWNTAIAPLN
                     TPRTIAADPHFQARFPWLPASEHVADMLPFPVHFVGEELPPPAPAPRAGQHNDSVLRE
                     LLGYSEEQLAALRQSGALG"
BASE COUNT          972 a         2153 c         2083 g          978 t
ORIGIN      
        1 gcgccgtgat cgcattgcca gccctcgccg cggcgcgtgc tcatgcgccc ggcaggaccg
       61 cggctcctgt cgaacaggct caccgtgcac ccgtgctgca cgagatgcgt ggcgcaggac
      121 agcccggcga tgccggcgcc gatgatggcg acgcggggtg cgggattcat ggacagactg
      181 cctcggagct cggggcgtct cagtacgcct gcgcccggcg gaccggatca acgaacggca
      241 cctctcaccc ggtcatcgcg cgcaggccgc gctcccacca atctagggcg tgtaccagat
      301 cggcgagctg taggcgcgct cctgcgtgct catcggtatc gagtccggca tctcgacgcc
      361 gaagaacttg cggtcgtagg cggtccagcg cggcgtgggg atctcgatca cacgcacgta
      421 ccagaacgcg cgctcggctg gatcgaaatc ggggtcggtc cataccgtcg acagctcggc
      481 cgcgccgatg ctgttgctcc agctcgcgtt cttcgcatcg accgtgttgc ccaccggagg
      541 caacctgccc ttgcgatcgg gtttgcgctc gcccgaccag gccacgtcat acacccgctc
      601 gtgcgtctcg cctttcgcgt cgagccagcc cttgacgatc tggatgcggt cgagcttgcc
      661 gctcaacgca tcgcgcgctg ccgccaccag gaaggtgggt gccgtgcccg cggatgcggc
      721 gggcagatcg ctgcccatcg gcacgccgcg cgcatagccc gtggccgcga ggtccggcgc
      781 gctcgcatcc gccgcggcga acccgtaacc gccgaagaag cgcagcgcga tgcgcggccc
      841 ggtggtcgcg tagacctcgc ggcgcttcat cgcctcgaag atcgccgcgc gcgtgttctc
      901 gcgcgcccat accgccatgt agcccgaagc cgccatgtcg gattcgcgga atgtcagttc
      961 cggtaccgtg ggattatcga acaggctctc gtgccagcgg cccggtgccg gttcaccctt
     1021 ggtgctcttg ccccagaaat tgttctcctc ggccgcggcg aagcccgtgt gcgcgtcggt
     1081 gctgccgatc aggccgaact tgaacgggtt tgcgccgagc gccgcctcct gctgcagccc
     1141 gatgcgcagc gccgaacgtg cgtactggaa gggctgcatc tgcggtgtct gcggcgtcat
     1201 gccgccgagg ttgatccggt cccagatctc gtagcccgcg aactcgtcct cgggggaatt
     1261 gagcggatgg gtctccgagt cacccttgta ctgcgtgact tccatcaccg gttcccagcg
     1321 gctgcgccgc tcggcatagt cgcggtcgaa ggccgcaccg ttgaagtcgc gcggcgcgaa
     1381 catcaggccg ccgctgacat tggagttgtg cgggatcgcg agcgcctggc cgccggtctg
     1441 ctgctcgtaa tcggcgaggt aggcccagag cttttccgga tcgctgctgt cgatggagct
     1501 gaagggcacg gtcttgccgg ccttgtcggc gccgtcggcg aacaccacca cgcggtgcag
     1561 gttgtcgccg ttgggcatcg aggtccactc gtaaccgacg atggccgtga agcggcccgg
     1621 ctcgttgaag cgctcggcgt tcgtcaccac ctcctgccag gtcgagctcc tgaactcggc
     1681 gctgccgatc acgtccttgt tggcgaagtt ggccgcaccc cactccatca gcatctgcgc
     1741 gttggcgcgc ccgcctttcg cgatgatgtc ggcccagcgc ctgccggtct cggtggcgag
     1801 caggttggga tctcccgcgc gcaggtgcgg gaacagcccg aggtattcgc tgtggtccgc
     1861 gaccaccagg aaatcgagcg gccgcgccag ccgcagcgcc atgccgttgt gcgccgtcac
     1921 ggtctcgccg cgcgcgaagc ggtaggcgtc ctgcgggccg acatcgcggt tgccgaagct
     1981 gccggcgtcg gccgacatgt tggtgtgcag gtgcgtgtcg ccccagagca ggcgctcggg
     2041 gaagctgcgc ccggcatagg gggaatagct ggattgctcc gcggccgacg tttcggtggc
     2101 aacggcgcag gcaagagcca gcgccgcgag gcgcgcggta ttcctgttca tgccgatctc
     2161 ctgcagtgtt ttttttatgc gttcctgacc accgtcaccg gcacggtcgc cagcttgatc
     2221 acctcggtcg cgaccgaacc catcaccgcg tggcgcaggc cggtgcgccc gtgcgtgccg
     2281 atcacgatca ggtcgaagtg catctcggcg gcgtagcggg tgacggtcgg cgcgatgtgg
     2341 cccaccgcga tatgagtggt gcagggctgc ccggccgcct cgagcgcggc gctcgcggcc
     2401 tggagctgcg cgaggccctc gtcgcggtag tactcctcga tcgcctcgcg cgcgatgaac
     2461 tgcttcacat gacccgattc gagcggcatc tgcacgttca gcaggtgcgc ctcgatgtcg
     2521 caatgcgcat cgcgcaggcg cagcacgtgc tccaccgctc gcagggaata ctcggagccg
     2581 tcgacggcta ccaggatgcg caggctcatg caggtactct ccgcgggttc aggcgttgcg
     2641 atcacgggac gcttcgagcg ggatctcgcg gatcggctcc aacccggcag gcctcctccg
     2701 ccgtccgagc caggtgccca gcgccaccac gaacagcgcg cccgcggcgg cactcgcgta
     2761 atgcagccag cccggcgcgc cctcgagata cggcttgacg gtgacgtcgg tgaccagcat
     2821 ctcgccggcg atccagccga gcagcgccgc accgagcgtg atcaccaccg ggaaacggtc
     2881 catcaggcgc agcacgaact ggctgcccca gacgatgatc gggatgctga tcaggatgcc
     2941 gaagaccacc agtgccatgc tgcccttggc ggcgccggcc acggcgatca cgttgtcgag
     3001 gctcatcacg gcatcggcca cgatgatggt cttgatcgcg cccatcagcg tgctgctgcc
     3061 cgcgacgttg gcgtgcccgt cctcgtcctc gggagtcagc agcttgacgc cgatccacag
     3121 cagcagcagc gccccgacca ccttcagcag cggcagcgcc agcaactgca gcgcgaagaa
     3181 gatcagcacc acgcgcaacc cgatcgcgcc ggccacgccc cagaagatgc ccttgcgccg
     3241 ctgctcctcg gggagcttgc ggcaggcgag cgcgataacg acggcattgt cgccgccgag
     3301 caggatgtcg atcaggatga tctggcctac cgacagccag aacgtggggt cgaacaagaa
     3361 ctccggcatc tgtcacccgt cgcatgcgga acacggtcga cgccggccgg cgtcacgagc
     3421 cgcgcaggat agagggactc gcaaggcaag tccaccgccc gctcaggcgg gcgcggccgg
     3481 cgtggacgcg ggcacataga gaacctgcag gtcgtacagc agcgcgggcc gctcctgccc
     3541 caccacgtgg atcgcgactt cgctggtgag cagcgtgccg cgccggtgcg cctgcacgtc
     3601 cttcaggcgc tgacgcaggt ggacgctgga gcccaccgtg accggcgaca ggaagcgcag
     3661 cccgccggaa ccgtaattcg cggcgctgcc gacattcagc agttcgaatg acggcggcgc
     3721 gcgcaggctc ggcatcagcg agagcagcag gaagccgtgc acgatcggcg caccgaaggg
     3781 gctctcgcgg cgcgcgcgct cgacatcgac gtgtatccac tgccggtcac cggtgagctc
     3841 ggcgaagcga ttcaccatgt cctgcgtgat cacgagcgct tcggaccagt cgccgaagtt
     3901 ctcgctcaac atggcgcgca gtgcatcgat gtcgcgcacg tcgatctggc ggggagaggc
     3961 accgggcatg accatgtcct gcattggatg tgtgagcaga tgataggatc ggcatcacgc
     4021 ggcgccaatc gaggcgcgcc ggctttgcca acggaaacct gaccatgtac cgcacactgc
     4081 tcgtcgaacg ccgcgaccgg gtcgccgtca tcacgcttga ccggcccgat cagctcaatg
     4141 cctacaccgt gcagatgggc gaggacctgg tgcatgcctt cgatgcgctg cgcgccgacg
     4201 actcggtgca cgtcgcggtg ctcacgggcg ccgggcgcgc gttctgcgcg ggcctggacc
     4261 tgaatgcgct gcgcgagacg ccggagccgg cactgccggc gctgggcgag gaacgcctga
     4321 tccgcagctt cggcgccgag ctgttcaact acccgaaacc cgtcatcgcg gccatcaatg
     4381 gcgcggccgc gggcatcggc gtcacgatga ccctgccctg cgacatccgc atcgccgcgg
     4441 ccggcgcgaa gctcgccttt ccgttcgcga agctcggcat cgtgcccgga ctgggcagct
     4501 ccttcctgct gccccgcctg ctcggacccg gacaggccaa gatcctcgcg ttgacgggcc
     4561 agaccatcag cgccgaggaa gcgcaggcca tcggcctggt ggatcgcgtc gtggcgccgg
     4621 aatcgctgct cgccgagaca ctggaactcg cccgggcgat cgcggcgaat tcgccgcaga
     4681 tcgtcgggct catcaaggaa gccctgaacg cgggtgccac agccacttcg atagacgccg
     4741 cgctggcctt cgagcatgcg cagaacgcgc gccggagcag gatcctctcc tgaacgacga
     4801 accatcgggc cggggaatcg cctcgccgcc gccctcaacc cagcgcgccg ctttgccgca
     4861 gcgccgcgag ttgctcctcg ctgtaaccca gcagctcgcg cagcacgctg tcgttgtgct
     4921 ggccggcgcg cggcgcgggc gcgggcgggg gcagttcctc gccgacgaaa tgcaccggaa
     4981 acggcagcat gtccgccacg tgttcgctgg ccggcagcca gggaaagcgc gcttggaagt
     5041 gcgggtcggc ggcgatggtg cgcggcgtgt tgagcggcgc gatcgcggta ttccactgca
     5101 ccgcgaacgc catccagtcc tgcgtggtcc tggtggcgaa gatatcgcgc agtatcgcct
     5161 gcagctcgcg attgccggtg gcgtgatcgc cgtagcgctt gcccggccag cgctcgaaga
     5221 ggtccagccg cccgactccg gtgcagaagt tcttccagaa ctcctgctcc gaggccatga
     5281 acaggatgtg tccgtcgcgc gatgcgtagt actggtaacg cacgcccttt tccatcccgg
     5341 ccgtacccgg ggcacgacgc acgtaatcat cggtctggtt gcccgtgact tcggattgcg
     5401 ggcgctcgta ggccttccag gtctcgatgc gcagccagtc catgtaggcg gccgcatcgc
     5461 tctgcccgag ttcgaggaag gcgcccgtgc cggtggcgcg cgcgcgcagc agcgccgcgc
     5521 agatcgacag cgccgcgatg agcggaccgg cgtgcatgcc gatcgagggg tgctcgggaa
     5581 tgaagcagaa accctcggcg tcgacacccg gcttcaccag gccggcccag ctgtcgaagg
     5641 ccacgccgtg tgccggcgca tccttgtagg ggccgctcat gccgtaaccg gacagggcac
     5701 agaacaccag cccgggcctg atcagtcgca gcgcctcata gcccaggccg cgacgcgcga
     5761 gcgcgccggg gcgcatggcc tcgatgacca cgtccgccct gcgcaccagg tcgcggaaca
     5821 ccgcgacgcc cgattccgtg cgcaggtcca gcgtcaggct cctcttgccg cgcgacacgt
     5881 ggtgatgcag cagcgagtcg ccctcgatga tcggccaggt caggctgcgc acgtagtcgc
     5941 ctccgggcgc ctcgaccttg atcacctcgg cgcccagatc cgccaggtgc gccgccgtgg
     6001 cgccgggact gagcagcgaa caatcgatca cgcgcacccc cgccaggggt gctgttgcat
     6061 cagccgtcat tgcagggctc cacgccggat gagagggaat gtgacgtcga agtgaagttg
     6121 cagcggcttc acggtgccgc ggcgaactcg gcgagcagcg tcgcacgcgc cgtagccgcc
     6181 tgctcc
//
LOCUS       DAORUQ010000177         6166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15075_length_6166_cov_6.287351, whole genome shotgun sequence.
ACCESSION   DAORUQ010000177 DAORUQ010000000
VERSION     DAORUQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15075_length_6166_cov_6.287351"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PLW13_13135"
     CDS             complement(<1..1496)
                     /locus_tag="PLW13_13135"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71371.1"
                     /translation="MKKTEMAARIACASALYVCVAGANAQQAGDDGRSAGLEEIVVTA
                     QKREENLQTVPIAVTALGTDMLESFRVTDIQDLNGLAPNLVMIDQGVRSIPTITIRGI
                     SSGVSNNAVDPKVGMYLDGVYIGRSVGAIFDMAEIERVEVLRGPQGTLFGRNATGGAI
                     SLITSAPTGEFGLKQDVSFGSNDAFRARTNLNLPSWGPLSVKFSYLHDEDEGTADNLI
                     GGQRIDLRPFDPDLGVLRYDDDLGSKDTDAFMFAARLALSEELTLDYKYDRTDSDTVG
                     NPTQQLGITGGALQPLLQLIDSLQPLYGGITNVSTDRLDDVAAATSVQPLEVFGHNLT
                     MNWDITDEVNFKSITAWREMEQDPNIYDLAATGGWKFTTAQLAGALSGDFGAIFNPAN
                     APGPNDSFFNLLTARSTSQRQFSQELQLTITKDLFDLVGGIFYFTEHSPGLDALGIMK
                     PITNGVVEPAWFDSIFGTGTNESIVDNDSVAIFAQGTWHATEQLDLTAG"
     gene            complement(1600..>2196)
                     /locus_tag="PLW13_13140"
     CDS             complement(1600..>2196)
                     /locus_tag="PLW13_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71372.1"
                     /translation="RQDCGQITDGACALVLASGEFAARHAARRGARLEQLPRIQGWGH
                     RNAGLRYHDKIARSRDEALVFPHVRDTITDAFRRAGIEGVGQLDGIETHDCFSVTEYM
                     ALDHFGITPPGQSWQAIESGLIERGGRLPVNPGGGLIGCGHPVGATGARMLFDAAKQV
                     TGQAGEMQVEGARRFATLNIGGSCATVASFVVGVDDPR"
     assembly_gap    2197..2296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2297..2664)
                     /locus_tag="PLW13_13145"
     CDS             complement(<2297..2664)
                     /locus_tag="PLW13_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71373.1"
                     /translation="MHSSTYILGGYQTDFARAWSREGLDISDMVREAALGALENCRLD
                     AQDVRSVHVGNAFGEAQRRQGHLGAMVAQVVPALWGVPAMRHEGACASSSLAVLAAMA
                     EIEAGRYDCVLVLGVEEFRNL"
     gene            complement(2730..3683)
                     /locus_tag="PLW13_13150"
     CDS             complement(2730..3683)
                     /locus_tag="PLW13_13150"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQY71374.1"
                     /translation="MSQFRNDSFEFVKQVSAEASADPCATSACAEQDATRAAAFAFVR
                     QLAQDLSDGSFELPAFPEVALRVREALNDPEVSVDKVARIVLSEPVLSARLLRIANSA
                     LMRRGSLEITDVRMAISRLGFEMVRNTAVSLAVDSAFKAPAHGPVRRQVESTRRHSVR
                     VAVLSYVLAKRLADGVKPDEAMLTGLLHAIGRFYILMRADAFPELFEHPDVLQGLMQD
                     WHPGVGRAIVEAWGFPEPIAVAVDEHEVLERDHAGRADVADIVLVANLLASRGEGAQA
                     DLPAMASIPACMKLKIDEQIGMRVLAESEQEILSMAQALGG"
     gene            complement(3912..4733)
                     /locus_tag="PLW13_13155"
     CDS             complement(3912..4733)
                     /locus_tag="PLW13_13155"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71375.1"
                     /translation="MQITGLEGKVAVVTGAGRMRSIGRPIAVELARAGCDVVLTGTGR
                     APGRYPDDERAAGWRDIESVAEEVRALGRRALALVSDVADPQAVDALVARTLDEFGRV
                     DIVVNNAGAARGEDRAAVVDLAIDAWRTVIDVNLNGTFYMSRAFGRALMEQGRGGSIV
                     NISSIGGKMMAAQTAAYAASKAGVHALTCAMAAELGPHGVRVNAVCPGIIDTFRMDDV
                     GRGEQWEKMIAAYVPLRRAGSGLDVANMVVYLCSEQGSWISGQLYSVDGGMVPGR"
     gene            complement(4832..>6166)
                     /locus_tag="PLW13_13160"
     CDS             complement(4832..>6166)
                     /locus_tag="PLW13_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HQY71376.1"
                     /translation="IASPVPMQYGLHELREIAHKLHPRAYVAAAHFRGEDFTARHAGA
                     FAPDTRLIALDHELIGADRAADESVIATGVSADEVYTICWTSGTTGTPKGVPRSHNQW
                     LSQTLAMHGIGLEPGMRMLCPFPMVNMASITGFFFPWLQLGGTLVLHHPMDVAVFLAQ
                     IRDERVAYTIAPPAVLNMLLQKRELLAGADLSSLRLVASGSAPLAPWMVRAFQEEFGI
                     VVVNIFGSNEGMAFASSGLDVPDPEQRATLFPRFGVEGLAWSNPIAARMRSRLVDIDS
                     GSVITEPGHPGEMQIQGPNVIDGYFDAPQGNAGVFSADGWFRSGDMFEIAPEDPRFYR
                     FTGRCKDIIIRGGMKISPDELDTLLGAHPQLAEAAVTGYPDAVMGERVCAVVVARPGE
                     QPTLESITQYLEGRHLARNKLPEKLVIVDALPRNPLGKVVRAALRGLVIDEP"
BASE COUNT          933 a         2140 c         2012 g          981 t
ORIGIN      
        1 cccgcggtga ggtcgagctg ctcggtggca tgccaggtgc cctgggcgaa gatggcgacc
       61 gagtcgttgt cgacgatcga ttcgttggtg ccggtgccga agatgctgtc gaaccacgcc
      121 ggctcgacga caccgttcgt gatcggcttc atgatcccca gcgcatccag gcccggcgag
      181 tgctcggtga aatagaatat gccgccgacc aggtcgaaca ggtccttggt gatcgtgagc
      241 tgcagttcct ggctgaactg gcgctgcgag gtcgagcgcg ctgtcagcag gttgaagaac
      301 gagtcgttcg gacccggcgc gttggccgga ttgaagatgg caccgaagtc gccgctcaac
      361 gccccggcca gctgggccgt ggtgaatttc cagccgccgg tggccgcgag atcgtagatg
      421 ttcgggtcct gctccatctc gcgccaggcc gtgatgctct tgaagttcac ctcgtccgtg
      481 atgtcccagt tcatcgtcag gttgtggccg aagacctcga gcggctgcac gctggtggcc
      541 gcggccacgt cgtcgagccg atccgtgctg acattggtga tgccgccgta caggggctgc
      601 agcgaatcga tgagctggag caagggctgc agcgctccgc ccgtgatgcc gagctgctgg
      661 gtcggattgc ccaccgtgtc gctgtcggtg cggtcgtact tgtagtcgag cgtcagctcc
      721 tcgctgagcg ccagcctggc ggcgaacatg aaggcatccg tgtccttgct gccgaggtcg
      781 tcgtcgtacc tcagcacgcc gagatcggga tcgaaggggc gcaggtcgat gcgctggccg
      841 ccgatcaggt tgtccgccgt gccctcgtcc tcgtcgtgca ggtaggagaa tttcacgctg
      901 agcgggcccc acgacggcag gttcaggttg gtgcgcgcac ggaaggcgtc gttgctgccg
      961 aacgagacat cctgcttcag gccgaactcg ccggtagggg ccgaggtgat caggctgatc
     1021 gccccgccgg tggcattgcg tccgaacagc gtgccctgcg ggccacgcag cacctcgacg
     1081 cgctcgatct cggccatgtc gaagatcgcg cccacgctgc gcccgatgta gacgccgtcg
     1141 agatacatgc cgaccttcgg atcgacggcg ttgttggata cccccgacga gatgccgcga
     1201 atggtgatgg tggggatgga acgcacgccc tggtcgatca tcaccaggtt cggtgccaga
     1261 ccgttcaggt cctggatatc ggtgacgcgg aagctctcca gcatgtccgt gcccagcgcg
     1321 gtcaccgcga tcggcaccgt ctgcaggttc tcctcgcgct tctgcgcggt gaccacgatc
     1381 tcctcgagcc cggcgctgcg cccgtcatcg cccgcctgct gcgcattagc gccggcaacg
     1441 catacataga gcgccgaagc acaggcaatc ctggcggcca tttccgtctt tttcatagtg
     1501 agtcatcccc tgtcagtgaa attgtcttta tggaaacgcg cgccgagcct aacgcgggca
     1561 cgatttcacg ccctcatgcc aacgaatgag gccgacgact caacgcgggt cgtcgacacc
     1621 caccacgaag ctcgccaccg tcgcacagga gccgccgatg ttcagcgtcg cgaagcgccg
     1681 cgcgccctcg acctgcatct cgcccgcctg ccccgtgacc tgtttcgccg catcgaacag
     1741 catccgcgcg ccggtcgcgc cgaccgggtg gccgcatccg atgaggcccc cgcccgggtt
     1801 caccggcagc ctgccgccgc gctcgatcag gccgctctcg atggcctgcc acgactgccc
     1861 cggcggcgtg ataccgaagt ggtccaacgc catgtactcg gtcaccgaga agcagtcgtg
     1921 cgtctcgatg ccgtccagtt ggcccacgcc ctcgatgccc gcgcggcgaa aggcatccgt
     1981 gatggtgtcg cgcacgtgcg ggaagaccag cgcctcgtcg cggctgcgcg cgatcttgtc
     2041 gtggtagcgc aggcccgcgt tgcgatggcc ccagccctgg atgcgcggca gctgctcgag
     2101 acgcgcgccg cggcgtgcgg cgtgccgggc cgcgaactcc cccgaggcca gcaccagcgc
     2161 gcaggcgccg tcggtgatct ggccgcagtc ctggcgnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnaggt tgcggaactc ctcgacgccc agcaccagca cgcagtcgta
     2341 gcgcccggcc tcgatctcgg ccatcgccgc cagcaccgcg agcgagctcg aggcgcaggc
     2401 gccctcgtgg cgcatcgcgg gcacgcccca cagcgcgggc accacctgcg cgaccatcgc
     2461 gccgaggtga ccctgcctgc gctgcgcctc gccgaaggcg ttgcccacgt ggacggagcg
     2521 cacgtcctgc gcgtcgaggc ggcagttctc cagcgcgccg agcgcggcct cgcggaccat
     2581 gtcggagatg tccagcccct cgcgcgacca cgcccgcgcg aaatcggtct ggtagccgcc
     2641 gagaatgtac gtggagctgt gcatgtgcgg cctgttcccc cgtgatcccc gcagcgcggg
     2701 caactgcagc cggttgcgca ggctggccct cagccgccca gcgcctgcgc catcgagagg
     2761 atttcctgtt ccgattccgc gagcacccgc atgccgatct gctcgtcgat cttcagcttc
     2821 atgcaggcgg gaatggaagc catggcaggc agatccgcct gtgcgccctc gccgcggctc
     2881 gcgagcaggt tcgccaccag cacgatgtcg gcaacatcgg cacgaccggc gtgatcgcgc
     2941 tccagcacct cgtgctcatc caccgccacc gcgatcggct ccgggaatcc ccacgcctcg
     3001 acgatggcgc gccccacgcc ggggtgccag tcctgcatca gcccctgcag cacgtccgga
     3061 tgttcgaaca gttcgggaaa ggcatcggcg cgcatcagga tgtagaaacg gccgatcgcg
     3121 tgcagcagcc cggtcagcat ggcctcatcg ggcttgacac cgtcggccag gcgcttggcc
     3181 agtacgtacg acagcaccgc cacgcgcacg ctgtggcgac gcgtgctctc gacctggcgg
     3241 cgaaccggtc cgtgcgcggg tgccttgaag gcgctgtcga cggccagcga caccgcggtg
     3301 ttgcgcacca tctcgaagcc gagacggctg atcgccatcc tgacgtcggt gatctcgagc
     3361 gagccgcggc gcatcagcgc cgaattcgcg atgcgcagca gccgcgccga cagcaccggc
     3421 tcggacagca cgatgcgcgc gaccttgtcc acgctgacct cgggatcgtt cagcgcctcg
     3481 cgcacgcgca gcgccacctc gggaaacgcc ggcagctcga agctgccgtc cgacaggtcc
     3541 tgcgccagtt gcctgacgaa tgcgaaggcg gcagcacgcg tggcgtcctg ttcggcgcaa
     3601 gcgctggtcg cgcacgggtc ggcggacgcc tctgcggaaa cctgtttgac gaattcgaag
     3661 gagtcgttgc gaaattgcga catgctggcg gtggcctgtg tgtccctgcg gttgccctgg
     3721 ccctgccccg ccacgctcgg cggctagccg cgcagtcccg cgcgcagaca ggaggttttt
     3781 tgtgcgacaa aggctagcac ctcgtcgcag gaacgcacct gcgcgcagga cgatttcgcc
     3841 ccatttgggt cgtgaatttt gcgcatcgcc gccgacgatg cgcactggcc gggcctgcgt
     3901 aggcgcgcgg ctcagcgccc gggcaccatc ccgccatcca cgctgtagag ctgcccgctg
     3961 atccacgaac cctgctcgct gcacaggtag acgaccatgt tggcaacgtc gaggccgctg
     4021 cccgcgcggc gcagcggcac gtaggcggcg atcatcttct cccactgctc gccgcgcccg
     4081 acgtcgtcca tgcggaaggt gtcgatgatg ccggggcata ccgcgttcac gcgcaccccg
     4141 tgcggcccga gctcggccgc catcgcgcag gtgagcgcgt gcacgccggc cttggaggcg
     4201 gcataggcgg cggtctgtgc cgccatcatc ttgccgccga tggaggagat gttgacgatg
     4261 ctgccaccgc ggccctgctc catcagcgcg cgaccgaagg cgcggctcat gtagaacgtg
     4321 ccgttcaggt tcacgtcgat cacggtgcgc caggcatcga tggcgagatc gacgacggcg
     4381 gcgcgatcct cgccgcgcgc ggcgccggca ttgttcacca cgatgtcgac gcggccgaac
     4441 tcgtccagcg tgcgcgccac cagcgcatcc acggcctgcg gatccgcgac gtcgctgacc
     4501 agcgcgagcg cccggcgacc gagcgcgcgc acctcctcgg caaccgattc gatgtcgcgc
     4561 cagccggcgg cgcgttcgtc atccgggtaa cgcccgggcg cgcgccccgt gcctgtcagc
     4621 acgacgtcgc aaccggcgcg cgcgagctcc accgcgatcg ggcgcccgat ggagcgcatg
     4681 cgcccggcgc cggtgacgac cgcgaccttc ccctcgagac ccgtgatctg cattcaccct
     4741 cctttcctgc cctgtgggtc ggcattcatg ccgacatgtg cctgccccac tgctgctgtc
     4801 gccatgaatg gcgacccacg tccatgcgga cctatggttc gtcgatgacc agcccgcgca
     4861 gcgccgcgcg caccactttg ccgagcggat tgcgcggcag cgcgtcgacg atgacgagct
     4921 tctcggggag cttgttgcgc gcgaggtgcc gcccctcgag atactgtgtg atgctctcca
     4981 gcgtcggctg ctcgccgggc ctcgccacca ccaccgcgca cacgcgttcg cccatcaccg
     5041 cgtcgggata gcccgtgacc gcagcctcgg cgagctgcgg atgcgcgccg agcagcgtgt
     5101 cgagttcgtc gggcgagatc ttcatgccgc cgcggatgat gatgtccttg caacgcccgg
     5161 tgaaacggta gaagcgcgga tcctccggcg cgatctcgaa catgtcgccg ctgcggaacc
     5221 agccgtccgc gctgaacacc ccggcgttgc cctgcggtgc atcgaaatag ccgtcgatca
     5281 cgttcggccc ctggatctgc atctcgccgg ggtggcccgg ttccgtgatc acgctgccgc
     5341 tgtcgatgtc gaccaggcgc gagcgcatgc gcgcggcgat cgggttcgac cacgcgaggc
     5401 cctcaacgcc gaagcgcggg aacagcgtcg cgcgctgctc gggatcgggc acgtcgaggc
     5461 ccgaggacgc gaaggccatg ccctcgttgg agccgaagat gttcacgacc acgatgccga
     5521 attcctcctg gaacgcacgc accatccacg gcgccagcgg cgcgctgccc gaggcgacca
     5581 gcctcaggct ggagaggtcc gcgcccgcca gcagctcgcg tttctgcagc agcatgttca
     5641 gcaccgcggg cggcgcgatc gtgtaggcca cgcgctcgtc gcggatctgc gccaggaaca
     5701 ccgcgacatc catcgggtgg tgcagcacca gcgtgccgcc gagctgcagc cacgggaaga
     5761 agaagcccgt gatgctcgcc atgttcacca tcgggaacgg gcacagcatc ctcatgccgg
     5821 gctcgagccc gatgccgtgc atcgccagcg tctgcgagag ccattgattg tggctgcgcg
     5881 gcacgccttt gggcgtgccg gtagtgccgg aggtccagca gatcgtgtag acctcatcgg
     5941 ccgacacgcc ggtcgcgatc accgactcgt ccgccgcgcg atcggccccg atcagttcgt
     6001 ggtcgagcgc gatcaggcgc gtgtcgggcg cgaaggcgcc ggcgtggcgc gccgtgaaat
     6061 cctcgccgcg aaaatgcgcg gccgcgacgt aggcccgcgg gtgcagcttg tgcgcgatct
     6121 cgcgcagctc gtgcaggccg tactgcatcg gcaccgggct cgcgat
//
LOCUS       DAORUQ010000178         6091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15357_length_6091_cov_5.165341, whole genome shotgun sequence.
ACCESSION   DAORUQ010000178 DAORUQ010000000
VERSION     DAORUQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15357_length_6091_cov_5.165341"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..3571
                     /gene="smc"
                     /locus_tag="PLW13_13165"
     CDS             65..3571
                     /gene="smc"
                     /locus_tag="PLW13_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQY71377.1"
                     /translation="MRLKTIKLAGFKSFVDPTTVKLPSNLCGVVGPNGCGKSNIIDAV
                     RWVLGEASAKHLRGESMADVIFNGSTGRKPVGQAAIELVFDNTDGTLGGEYASYAEIA
                     IRRTVSRDGLSHYYLNGTRCRRRDITDIFLGTGLGPRSYAIIEQGMISRLIEARPEDL
                     RVYIEEAAGISKYKERRKDTENRIRRTRENLERLTDFREELERQLQHLKRQAAAAERY
                     SELKTEERRISAELLALQWRQLDAQGAGLREGVLRHEVELAAVNARHTRVDADIEKAR
                     EQHSGLNEALGGVQERYYAIGADIARTEQSVQLQQERARQLRADLEQTRRSREEAERH
                     LRDDEGRLARLGAELEEVIPALAEAEAAEASAQEALALADEAMHQWQLEWEEFSSASE
                     KPRQEVEVQQSRIRHLEEALAQAQQRLERLEHEAAELDTTAAETERETLDERVQELEE
                     ISRGWQERADTVRAQIDVARASITQFTGQLDEVRGELQEQRGRFASLQALQQAALGQT
                     DEVVTGWLQAQGLARNQRVAERLTVEPGWETAVETVLGSHLQAVCVDDMNPLAAALER
                     FGAGTLGLFSPRTATGAGAAPQGLAAKVRAPFDVGGLLAGILVADSLAQALALQEGLA
                     PGESVVTPEGIWLGRDWLRLARDTDARAGVIQRSRELAGLEAAMDAGREREALLLETL
                     EHSREELRELEAGMLQQQQELRAATARYTEARSALSACNARLEQMLLRDERNRSEQQQ
                     TRERRDAQRAELQGARSVLEAALDAMHRDSQRRETLQLQRDMARQALEAARQKARQDK
                     DSAHQLAMRRQSLGSQTESLRAAIARIQGQFAQLEARTGALAAQIEATEEPIAEGREL
                     LEAQLQDRLAVEGELTAARARVQEVEHALREAERSRHAIEQETEAVRSALEQERIAAQ
                     GVQVRLGAIVEQLHKGHADLAAVLAGLPEDAQETAWQAQLERLGGQLARLGPINLAAI
                     EEHRQQSERKNYLDAQNADLVEALETLENAIRKIDRETRTRFKDTFDKVNSGLQELFP
                     RVFGGGHAYLELTGDDLLDTGVAIMARPPGKRNATIHLLSGGEKALTAISLVFSIFRL
                     NPSPFCMLDEVDAPLDDANVGRFARLVREMSETVQFIVITHNKITMEMAHHLMGVTMQ
                     EPGVSRLVSVDVEEAVALAHTA"
     gene            3679..4572
                     /gene="zipA"
                     /locus_tag="PLW13_13170"
     CDS             3679..4572
                     /gene="zipA"
                     /locus_tag="PLW13_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HQY71378.1"
                     /translation="MELSVQDWLIVIGALLIVGVLLDAFRRYRNESRNPIRMGRKPGF
                     WKLGDGFRDDAEAPALPNAELPGGGARVRDRGGIGHDNRGMDDFEAPAPRVAPRVEPV
                     LEPEPPAVHVPAPPQPRRETPPEQAVLPGTEPITAPVREKRARAAAAPAPETDSEVVV
                     IHVLAREAGGFPGEALVKIFHACDVRHGEMDIFHRHEQEKGQGPVQFSVANVSRPGTF
                     DIAAMDALHLPGLSFFLRLPGPTRPLEAFDCMLETARVVARNLDGELQDESHSVFTTQ
                     TAEHCRQRIREFAHRNSRARR"
     gene            4649..>6091
                     /gene="ligA"
                     /locus_tag="PLW13_13175"
     CDS             4649..>6091
                     /gene="ligA"
                     /locus_tag="PLW13_13175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQY71379.1"
                     /translation="MSPGADAAGEIERLREQVRHHNHRYYVLDDPLIPDSEYDRLLHR
                     LQELERAHPELVSADSPTQRVGAGPLAVFATVRHEIPMLSLDNVFSDDELRAFDRRVR
                     ERLGDEAGVDYCCEPKLDGVAVSIVYEHGVLVRAATRGDGTTGEDITANVRTIASVPL
                     RLPAAVPPQRLEVRGEVYMPHRGFEEMNRRAAQRGEKTFVNPRNAAAGSLRQLDPRIT
                     AARPLEFCAYGLGVLDGASMPSTQSGALAWLRELGVRTSGELRVARGVEECLAYYAQM
                     QERRASLRYDIDGIVFKVDSVALQQRLGFVSRAPRWAVAHKFPAQEEMTTLLGVEFQV
                     GRTGAITPVARLAPVFVGGVTVSNATLHNMDEVRRLGLMVGDTVIVRRAGDVIPQVVR
                     VLEEQRAPHARAIALPVNCPVCGSPVEQGEGEAVARCSGGLVCAAQQKETIRHFASRR
                     ALDFEGLGEKLIEQLVDQGVVRNVADVYALD"
BASE COUNT          959 a         2060 c         2277 g          795 t
ORIGIN      
        1 tagacttgcg cgctttcctc gtgccccggg cccgcccggc ggccttctgt ccggcggatc
       61 cgcaatgcga ctcaagacca tcaagctggc ggggttcaag tccttcgtcg accccacgac
      121 cgtgaagttg ccgagcaacc tgtgcggggt ggtcggtccc aacggctgcg gcaagtccaa
      181 catcatcgat gccgtgcgct gggtgctcgg ggaggcctcc gcgaagcatc tgcgcggcga
      241 gtccatggcc gacgtcatct tcaacggctc gaccgggcgc aagccggtgg ggcaggcggc
      301 gatcgaactg gttttcgaca acaccgacgg cacgctcggc ggcgagtacg cgagctatgc
      361 cgagatcgcg atccgccgta ccgtcagccg cgacgggttg tcgcactact acctcaacgg
      421 cacgcggtgc cggcggcgcg atatcaccga catctttctc ggcaccggcc tcggcccgcg
      481 cagctacgcg atcatcgagc agggcatgat ctcgcgcctg atcgaagcca ggcccgagga
      541 tctgcgcgtc tacatcgagg aggccgcggg catctccaag tacaaggagc ggcgcaagga
      601 taccgagaac cgcatccgcc gcacgcgcga gaacctcgag cgcctgacgg actttcgcga
      661 ggaactcgag cgccagttgc agcacctcaa gcgccaggcc gcggccgccg agcgctactc
      721 ggagctgaag accgaggagc ggcgcatcag cgccgagctg ctggcgctgc agtggcgcca
      781 gctcgatgcc cagggcgcgg gactgcgcga gggtgtgttg cgccacgagg tcgaactcgc
      841 ggcggtcaat gcgcgccaca cgcgcgtcga cgcagacatc gagaaggctc gcgagcagca
      901 ttccgggctg aacgaggcgc tcggcggtgt tcaggagcgc tattacgcca tcggcgccga
      961 tatcgcgcgc accgagcagt cggtgcagct gcagcaggaa cgcgcgcggc agttgcgcgc
     1021 ggatctcgag cagacgcggc gctcgcgcga ggaggccgag cgtcacctgc gcgatgacga
     1081 gggccgcctc gcgcgcctgg gcgccgagct cgaggaggtg ataccggcgc tcgccgaggc
     1141 cgaggccgcc gaggcgagcg cccaggaggc gctggcgctg gccgacgagg ccatgcacca
     1201 gtggcagctc gagtgggagg agttcagctc cgcctccgaa aagccgcgcc aggaagtcga
     1261 ggtgcagcag tcgcgcatcc gccatctcga ggaggcgctg gcgcaggcgc aacagcgcct
     1321 ggaacggctg gagcacgagg ccgccgagct cgacaccacg gccgccgaga cggagcgcga
     1381 gacgctggac gagcgcgtgc aggagctcga ggagatcagc cgcggctggc aggagcgtgc
     1441 cgacacggtg cgcgcgcaga tagacgtggc gcgcgccagc atcacgcagt tcaccgggca
     1501 gctcgacgag gtgcgcggcg agttgcagga gcagcgcggg cgcttcgcct cgctgcaggc
     1561 gttgcagcag gccgccctgg ggcagaccga cgaggtcgtt accggctggc tgcaggcgca
     1621 gggcctggcg cgcaaccagc gcgtggccga gcgcctgacg gtcgagccgg gctgggaaac
     1681 cgcggtggag accgtgctcg gcagccacct gcaggcggtg tgcgtcgatg acatgaaccc
     1741 gctggccgcg gcgctggagc gcttcggcgc cggcacgctg gggctgttca gcccgcgaac
     1801 ggcgacaggc gccggggcgg cgccgcaggg cctcgcggcc aaggtgcgcg cgccattcga
     1861 tgtcggcgga ttgctggccg ggatccttgt cgcggactcg ctggcgcagg cgctggcgct
     1921 gcaggaaggg ctcgcaccgg gcgagtcggt ggtcacgccg gagggcatct ggctcggtcg
     1981 cgactggctg cgcctggcgc gcgacaccga tgcgcgtgcc ggcgtgatcc agcgcagccg
     2041 cgagctcgcc gggctcgagg ccgcgatgga cgccgggcgc gaacgcgagg ccctgctgct
     2101 ggagacgctg gagcactcgc gcgaggaact gcgcgagctc gaggccggga tgttgcagca
     2161 gcagcaggag ctgcgcgcgg cgaccgctcg ctacaccgag gcgcgttcgg cgctcagcgc
     2221 ctgcaacgcg cgcctggaac agatgctgct gcgcgacgag cgcaaccgct ccgagcagca
     2281 acagacccgc gagcggcgcg acgcacagcg cgccgagttg cagggcgcgc gcagtgtgct
     2341 cgaggccgcg ctcgacgcca tgcatcgcga cagccagcgg cgcgaaacgc tgcagctgca
     2401 gcgcgacatg gcccgccagg cgctcgaggc cgcccggcag aaggcgcgtc aggacaagga
     2461 ttcggcgcac cagctggcga tgcgtcgcca gtcgctgggc tcgcaaaccg agagcctgcg
     2521 cgcggcgatc gcgcgcatcc agggccagtt cgcccagctc gaggcgcgca ccggggcgct
     2581 ggccgcgcag atcgaggcca ccgaggagcc gatcgccgag gggcgcgagc tgctcgaggc
     2641 gcagctgcag gaccgtcttg ccgtcgaggg cgagctgacg gccgcgcgcg cccgggtgca
     2701 ggaggtcgag cacgcgctac gcgaggccga gcgcagccgc cacgccatcg agcaggagac
     2761 cgaggccgtg cgcagcgcgc tggaacagga gcgcatcgcg gcgcagggcg tgcaggtgcg
     2821 cctgggcgcc atcgtcgagc agctgcacaa ggggcacgcg gatctcgcgg ccgtgctggc
     2881 aggcctgccc gaagacgcgc aggagacggc gtggcaggcg cagctggaac gcctcggggg
     2941 ccaactggcg cgactcgggc cgatcaacct cgcggcgatc gaggaacatc gccagcagtc
     3001 ggagcgcaag aactacctcg atgcgcagaa cgccgacctg gtcgaggcgc tggagacgct
     3061 ggagaatgcg atccgcaaga ttgaccgcga gacgcgcacg cgcttcaagg acacgttcga
     3121 caaggtcaac agcggtctgc aggagctgtt cccgcgcgta ttcggaggcg ggcacgccta
     3181 cctcgaactg accggcgacg acctgctgga taccggggtc gcgatcatgg cgcggccccc
     3241 gggcaagcgc aatgcgacga tccacctgct ctcgggcggg gagaaggcgc tcacggcgat
     3301 ctcgctggtg ttctcgatct tccgcctgaa cccgtcgccg ttctgcatgc tcgacgaggt
     3361 cgatgccccg ctcgatgacg ccaacgtcgg gcgattcgcg cggctcgtgc gggagatgtc
     3421 cgagacggtg cagttcatcg tgatcacgca caacaagatc acgatggaaa tggcgcatca
     3481 cctgatgggc gtcacgatgc aggagccggg cgtctcgcga ctggtcagcg tggatgtcga
     3541 ggaggccgtg gcgctggcgc acacggcgtg aaaggcgttg ccggcgcgat gttctatagt
     3601 gacgtttggt tatagaatgc cgcggcaacg cgggaacaga caggccattc cacccggttc
     3661 acaggatctg ctagcggcat ggagttgagt gtacaggact ggttgatcgt cattggcgcg
     3721 ctcctcatcg tgggggtgct gctcgatgcg ttcaggcgct atcgcaacga gagccgcaac
     3781 ccgattcgca tgggccgcaa gcccggcttc tggaagctgg gcgacggttt ccgcgacgat
     3841 gccgaagccc cggcgctgcc caacgccgag ctgccgggcg gcggcgcgcg cgtgcgcgac
     3901 cgtggcggca tcggccacga caaccgcggc atggacgatt tcgaggcgcc ggcgccacgg
     3961 gtggcaccgc gcgtcgagcc ggtgctggaa cccgaaccgc cggccgtgca cgtccccgcc
     4021 cccccgcagc cgcgccgcga gacgccgccg gaacaggcgg tccttcccgg caccgagccg
     4081 atcacggccc ccgtgcgcga gaagcgcgcc agggccgctg cggcgccggc gccggagaca
     4141 gacagcgaag tcgtggtgat ccatgtgctg gcgcgcgagg ccggggggtt tcccggcgag
     4201 gcgctggtga agatcttcca cgcctgcgat gtccgccacg gcgagatgga tatcttccac
     4261 cgccacgagc aggagaaggg ccaggggccg gtgcagttca gcgtggcgaa cgtgtcgcgc
     4321 cccggcacct tcgacatcgc ggccatggac gcgctgcacc tgcccgggct cagtttcttc
     4381 ctgcgcctgc ccgggccgac gcgcccgctc gaggccttcg actgcatgct cgagacggcg
     4441 cgcgtcgtgg cgcgcaatct cgacggcgaa ctgcaggacg agagccacag cgtgttcacc
     4501 acgcagaccg ccgagcattg ccgccagcgc atccgcgagt tcgcgcaccg caactcgcgc
     4561 gcgcggcgct agccttgtcc ccgccggggc cggggccggg gccggggccg gggccggggc
     4621 cgggggtcgc gggcgcggag ccgccgcgat gagccctggc gccgatgccg ccggggagat
     4681 cgagcggctg cgcgagcagg tccgccacca caaccaccgc tactacgtcc tcgacgaccc
     4741 gctgatcccg gacagcgagt acgaccggct cctgcaccgg ctccaggagc tcgagcgcgc
     4801 gcatcccgaa ctggtcagcg cggattcccc gacgcagcgc gtcggcgccg ggccgctggc
     4861 ggtcttcgcg acggtgcgtc acgagatccc gatgctgtcg ctggacaacg tcttcagcga
     4921 cgatgaactg cgcgccttcg atcggcgcgt gcgcgagcgc ctcggcgacg aggcgggcgt
     4981 ggactactgc tgcgagccca agctcgacgg tgtggcggtc agcattgtct acgagcacgg
     5041 cgtgctggtg cgcgccgcga cgcgcggcga cggcacgacg ggcgaggaca tcaccgcgaa
     5101 cgtgcgcacc atcgcctcgg taccgttgcg cctgccggcg gcggtcccgc cgcagcgcct
     5161 cgaagtgcgc ggcgaggtgt acatgccgca ccggggcttc gaggagatga accggcgcgc
     5221 cgcgcagcgc ggcgagaaga ccttcgtgaa cccgcgcaac gccgccgccg gcagcctgcg
     5281 ccagctcgac ccgcgcatca ccgcggcgcg cccgctggag ttctgcgcct acggtctggg
     5341 cgtgctcgat ggtgcctcga tgccgtccac gcagtcgggg gcgctggcgt ggctgcgcga
     5401 gctcggtgtg cgcaccagtg gcgagttgcg tgtcgcgcgc ggtgtcgagg agtgcctggc
     5461 ctattacgcg cagatgcagg agcggcgcgc gtccctgcgc tacgacatcg acggcatcgt
     5521 gttcaaggtc gacagcgtgg cgctgcagca gcggctcggc ttcgtgtcgc gggcgccgcg
     5581 ctgggcggtc gcgcacaagt ttccggcgca ggaggaaatg accacgctgc tcggggtgga
     5641 gttccaggtc gggcgcaccg gcgcgatcac gccggtggcg cggctggcgc cggtgttcgt
     5701 gggcggtgtg acggtcagca acgccacctt gcacaacatg gacgaggtgc gccgcctcgg
     5761 cctgatggtc ggcgacaccg tgatcgtgcg ccgtgccggc gacgtgatcc cgcaggtggt
     5821 gcgcgtgctc gaggagcagc gtgcgccgca cgcgcgcgcg atcgcgctgc ccgtgaactg
     5881 cccggtgtgc gggtcgccgg tcgaacaggg cgagggcgaa gcggtggcac gctgctccgg
     5941 cgggctggtg tgcgccgcgc agcagaagga gaccatccgc cacttcgcct cgcgccgcgc
     6001 gctggacttc gaggggctgg gcgagaagct gatcgagcag ctggtcgacc agggcgtggt
     6061 gcgcaatgtc gccgacgtct acgcgctcga c
//
LOCUS       DAORUQ010000179         6052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15518_length_6052_cov_5.667167, whole genome shotgun sequence.
ACCESSION   DAORUQ010000179 DAORUQ010000000
VERSION     DAORUQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15518_length_6052_cov_5.667167"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3066
                     /locus_tag="PLW13_13180"
     CDS             <1..3066
                     /locus_tag="PLW13_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HQY71380.1"
                     /translation="SPEMRPLTLAMTAFGPFAGTASIDFRRLGENPLFLINGPTGAGK
                     TSILDAICFALYGETTGGERQGAEMRSHHAAPELLTEISFEFELAGKHYRIRRVPDQE
                     RPKARGEGTTQHKAEAQLDELGADGSARNLVPRKVGEANQRVMELTGLDADQFRQVMV
                     LPQGRFRELLLAPSQEREAIFQQLFQTHVYARLERALKDGANALSAEVERRGERSRGL
                     LEGCGLASGDALATALSEQLDQIAVAEAARDAALAQHGEVQRALEAARLLEQRFVAHD
                     GARGALERLVAEEPLRAQERSRAGAAAAARALAPHHESVAQRAAEHERATGRWREAEA
                     ALARARDASGHADAAWREATAQEPELLRLRDEMLRLRALEPVAIGLQQGERLLRERRA
                     ERARADAALETARAAHEAAAKALADVDARITGLRQRTEALAQAPLARERCDARLLVRR
                     RQEELTATRDDLRAAGDTAARRLDAAALAQRRAGESLRALRQAWQAGQAVVLARELRH
                     GAPCPVCGSAEHPAPAAGAQEVPAPDTLELALEVDERAREALEGARIERVRVDEAIAA
                     QEAELARLRDELGDHAREPLAALEQQAATLAADVQAHDLAQAELRDAERARADCIERV
                     TQLASATDSMRLAAHTAERAEGEAAAAFAAQQRQLPEALRAPGAVAEALRVATAGAER
                     LGTALEAARSRQQQASNGLVGAQVEAAGRHDEMQRAAALLQEAEATWQQLLAASPFAD
                     TTAFRQALLEPGELQRLEQEIRDRENALLLARRALEDTAGAIAGAERPLLPALEQAEL
                     VARSARDAAVGDVRVLGERQAALERTQHALAQLLAEQAALEAEYAVLGRLARIANGGN
                     AHNLSLQRFVLSVLLDEVLVEAGHRLRLMSRGRYQLSRREEVRDARSKSGLELDVEDA
                     YTGRVRSVATLSGGESFMAALALALGLSDVVQAHSGGIRLDTLFIDEGFGSLDADALE
                     LAIRTLVDLQAGGRMVGIISHVPELKQQMDVQIVVEPGASGSHLRLVVP"
     gene            3081..3503
                     /locus_tag="PLW13_13185"
     CDS             3081..3503
                     /locus_tag="PLW13_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71381.1"
                     /translation="MRGNRTITTALVQTRIRRDRDGGFRALVSQQFDGRTLRRELQFS
                     VSPEQLAESGGAAMYCPSCRARRRCDAITFTRVGPENGWSRRVRMCRSCFYHFVTAEV
                     SESLILEMGEMLDEKDEGGAPAGPGASTVIPFRRGRPR"
     gene            complement(3500..4021)
                     /locus_tag="PLW13_13190"
     CDS             complement(3500..4021)
                     /locus_tag="PLW13_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71382.1"
                     /translation="MTQEIYQRAAQKIIAFHREAEQRNEWTFFVDEMYAPECVYVCEY
                     AGTMRVCATGIDEIKATHYGRDMQRGWEGWTFPYLDVYVGNEGRVVTHWMNRGPGKRP
                     DGSWFETPGISFITFNGAGRITAQFDVFDLAHQMHLCDELEAHGLLSAELKQNWVIPM
                     KTRLITMLQANMP"
     gene            complement(4040..4534)
                     /locus_tag="PLW13_13195"
     CDS             complement(4040..4534)
                     /locus_tag="PLW13_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71383.1"
                     /translation="MVRPKSEGRVEIDASGDQAGALCELLRDAQHIVRIFSDALSPQL
                     FDHAGLASELSRVARQGRACEVRILLKDSQFLTRRTHRIGALHQRLVSSVLLRKLTYC
                     PDHYVANYVLVDDGGIFFIPNEDDKVCFWNRDDRPLVRHYTEQFDELWQRSSPDPELR
                     FMPM"
     gene            complement(4545..5930)
                     /gene="purB"
                     /locus_tag="PLW13_13200"
     CDS             complement(4545..5930)
                     /gene="purB"
                     /locus_tag="PLW13_13200"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQY71384.1"
                     /translation="MQLSELSAISPLDGRYGAKTASLRPIFSEFGLIRFRVLVEVRWL
                     QCLAALPGVPEFGPFDTATSAALDAIASGFALADAERVKAIEATTNHDVKAVEYFLKE
                     RIHAIPGAAAGLEFVHFACTSEDINNLSHALMVNAARAEVMLPAMDRIIEALAAMAED
                     WAAVPLLSRTHGQPATPSTMGKEIANVAYRLHRQRRQVAGVRLLGKINGAVGNYNAHL
                     AAYPAVDWEANARDFVTSLGLDWNPYTTQIEPHDCLAELFAAMTRFNTILLDFARDVW
                     GYISIGYFRQRTRAGEVGSSTMPHKVNPIDFENAEGNLGLANAVFGHLIEKLPISRWQ
                     RDLTDSTVLRNLGGAFAQSLIAYEATLKGIGKLELDATRLLADLDANWEVLAEPIQTV
                     MRRYAVPEPYEKLKALTRGQRLGAAEMRAFIDGLAIPAEARRSLAEMTPASYIGNAAA
                     QARRIRDHLHD"
BASE COUNT          879 a         1986 c         2280 g          907 t
ORIGIN      
        1 agtcctgaga tgcgcccgct caccctcgcc atgaccgcct tcggtccctt cgccggcacc
       61 gcgagcatcg atttccgccg cctcggcgag aatccgctgt tcctgatcaa cggtcccacc
      121 ggcgccggca agaccagcat cctcgatgcg atctgcttcg cgctctacgg cgagaccacc
      181 ggcggcgagc gccagggcgc cgagatgcgc tcgcaccacg cggcccccga gctgctgacc
      241 gagatcagct tcgagttcga gcttgcggga aagcattacc gcatccgccg tgtgcccgac
      301 caggagcggc cgaaggcgcg cggcgaaggc acgacgcagc acaaggccga ggcgcagctc
      361 gatgagctcg gcgccgacgg cagcgcgcgc aacctggtgc cacgcaaggt cggcgaggcg
      421 aaccagcgcg tgatggagct caccgggctc gatgccgacc agttccggca ggtcatggtg
      481 ctgccgcagg gcaggtttcg cgagttgctg ctggcgccct cgcaggagcg cgaggcgata
      541 ttccagcagc tgttccagac ccacgtctat gcgcgcctgg aacgcgcgct gaaggacggc
      601 gcgaacgcgc tgagtgccga ggtcgagcgg cgcggcgaac gcagtcgcgg cctgctggag
      661 ggttgcgggc tggcatcggg cgatgcgctc gcgacggcgc tgagcgagca actcgatcag
      721 atcgcggtgg ctgaagcggc gcgcgacgcc gcgctcgcgc agcacggcga ggtgcagcgt
      781 gcgctggagg cggcgcgcct gctcgaacag cgcttcgtgg cgcacgacgg cgcgcgcggc
      841 gcgctcgaac gcctcgtggc cgaagagccg ttgcgcgcgc aagaacgcag cagagccggt
      901 gccgcggcgg cggcgcgcgc gctggcgccg catcacgaga gcgtggcgca acgcgcggcc
      961 gagcacgagc gcgccaccgg gcgttggcgc gaagccgagg cggcgctggc gcgcgcgcgg
     1021 gacgcatccg ggcacgccga cgctgcgtgg cgcgaggcca cggcacagga gccggagctg
     1081 ctgcgcttgc gtgacgaaat gctccggttg cgcgcgctgg aacccgtcgc catcgggttg
     1141 caacagggcg agcgcctgtt gcgcgagcgt cgcgccgagc gcgcgcgggc cgatgcggcg
     1201 ctggagacgg cgcgggccgc gcacgaggct gccgccaagg ccctggccga cgtcgatgcg
     1261 cgcatcaccg gcttgcgcca gcgcacggag gcgctggcgc aggcgccgct ggcgcgcgag
     1321 cgctgcgacg cacggctgct cgtgcgccgg cggcaggagg aattgacggc aacgcgggat
     1381 gacttgcgcg cggcgggtga tacggccgcg cggcggctcg acgctgccgc cctcgcgcag
     1441 cgccgggcag gtgaatcctt gcgcgccctg cgccaggcct ggcaggccgg gcaggcggtg
     1501 gtgctggcgc gcgagctgcg ccacggcgcg ccgtgtccgg tgtgcggcag cgcggagcat
     1561 cccgcgcccg ccgccggggc gcaggaggtg ccggcgccgg atacgctgga gcttgcgctc
     1621 gaagtcgacg agcgggccag ggaggcgctc gaaggggcgc ggatcgaaag ggtccgggtc
     1681 gacgaggcga tcgcggcgca ggaggcggaa cttgcgcgcc tgcgcgatga gctgggcgat
     1741 catgcccggg agccgctggc agcgcttgaa cagcaggccg ccacgctggc tgctgatgtg
     1801 caggcgcacg acctggcgca ggcagagctg cgtgatgccg agcgcgcccg cgctgattgc
     1861 atcgagcggg tcacgcagct cgcgagtgcc acggactcca tgcgcctggc ggcgcacacg
     1921 gccgagcgcg ccgaaggcga ggcagccgcc gccttcgcgg cgcagcagag gcaactgccc
     1981 gaggcgctgc gcgcgcccgg cgccgtggcc gaggcgctgc gggtggcgac ggctggcgcc
     2041 gagcgcctcg gcacggcgct cgaagcggca cgctcgaggc agcagcaagc gagtaacggc
     2101 ctggtcgggg cgcaggtgga ggccgctggc aggcatgacg agatgcagcg tgcggcggcg
     2161 ctgctgcagg aagccgaggc gacgtggcag cagcttctgg cggcgagtcc cttcgccgac
     2221 accacggcgt ttcgccaggc cctgctcgaa cccggggagc tgcagcggct ggagcaggaa
     2281 atccgcgacc gggagaacgc gctgctgctg gcgcgtcgag cgctcgagga taccgccggc
     2341 gccatcgcgg gtgccgagcg accgctgttg ccggcgctgg aacaggcgga actcgtggcg
     2401 cgcagcgcgc gcgatgcggc cgtcggcgac gtgcgggtgc tgggggagcg ccaggcggcg
     2461 ctggagcgaa cgcagcacgc gctggcgcag ctgctcgcgg agcaggccgc gctggaggcc
     2521 gaatatgctg tgctggggcg cctcgcgcgc atcgccaacg gtggcaatgc gcacaacctc
     2581 agcctgcagc gcttcgtgct cagcgtgctg ctcgacgagg tgctggtcga ggccgggcac
     2641 cggttgcggt tgatgagccg gggccgctac cagctgtcac ggcgcgagga ggtgcgtgat
     2701 gcgcgcagca agtcgggcct cgagctcgat gtcgaggatg cctacaccgg gcgcgtgcgc
     2761 tcggtcgcca cgctttccgg tggcgagagc ttcatggccg cgctggcgct ggcactcggg
     2821 ctttccgacg tggtgcaggc acactcgggc ggcatacggc tcgatacgct gttcatcgac
     2881 gagggcttcg gcagcctgga tgccgatgcg ctggagctgg cgattcgcac cctggtcgat
     2941 ctgcaggcgg gcgggcgcat ggtgggcata atctcgcacg tgcccgaact gaaacagcag
     3001 atggacgtgc agatcgtggt ggaaccgggc gccagcggca gccatctgcg gctggtcgtg
     3061 ccgtagggag tatgcagcgc atgcgaggca accgcacgat cacgaccgcg ctggtgcaga
     3121 cgcgcatccg ccgcgatcgt gacggcggct tccgtgcgct ggtgagccag cagttcgacg
     3181 gtcggacgct gcgccgggag ctgcagttca gcgtgagtcc ggagcagttg gcggagtccg
     3241 gcggcgcggc gatgtattgc ccgagctgcc gcgcgcggcg tcgctgcgat gcgatcacgt
     3301 tcacgcgcgt cgggccggag aacggctggt cgcggcgcgt gcgcatgtgc cgcagctgct
     3361 tctaccactt cgtcaccgcc gaagtgtccg agtcgctgat cctcgagatg ggcgagatgc
     3421 tcgacgagaa ggacgagggc ggcgcgcccg ccggtcccgg cgcctcgacg gtgattccct
     3481 tccggcgcgg gcggccgcgc tagggcatgt tcgcctgcag catggtgatc agcctggtct
     3541 tcatcggtat cacccagttc tgcttcagtt cggccgagag caggccgtgc gcctcgagct
     3601 cgtcgcacag atgcatctgg tgcgccaggt cgaacacgtc gaactgcgcg gtgatcctgc
     3661 ccgcgccgtt gaaggtgatg aaggagatcc cgggcgtctc gaaccaggag ccgtcgggcc
     3721 gcttgcccgg gccgcgattc atccagtgcg tcaccacgcg gccctcgttg ccgacgtaga
     3781 catcgagata gggaaaggtc cagccttccc agccgcgctg catgtcgcga ccgtagtgcg
     3841 tggccttgat ctcgtcgatg ccggtggcgc acacgcgcat ggtgccggcg tactcgcaga
     3901 cgtagacgca ctcgggcgcg tacatctcgt cgacgaagaa ggtccactcg ttgcgctgct
     3961 ccgcctcccg gtgaaaggcg atgattttct gcgcggcccg ttgatagatt tcctgcgtca
     4021 cgcggtcggt ctcctgtgat tacatgggca tgaagcgcag ttcgggatcg gggctgctgc
     4081 gctgccagag ctcgtcgaac tgctcggtgt aatgcctgac gagggggcgg tcgtcgcggt
     4141 tccagaagca caccttgtcg tcctcgttcg ggatgaagaa gatcccgccg tcgtcgacca
     4201 gcacgtagtt cgccacgtag tggtccgggc aatacgtgag cttgcgcaac agcaccgagg
     4261 agaccaggcg ctggtgcagg gcgccgatgc ggtgggtgcg ccgcgtcagg aactggctgt
     4321 ccttgagcag gatgcgcacc tcgcaggcgc gaccctggcg cgccacgcgc gacagttcgc
     4381 tcgcgagacc ggcatggtcg aagagctgcg ggctcaaggc gtcgctgaag atgcgcacga
     4441 tatgctgcgc gtcccgcagc agttcgcaca gcgcgccggc ctggtcgccg ctggcatcga
     4501 tctcgacacg cccctcgctc ttcggtcgca ccatcgtcct cgcctcagtc gtgcaggtga
     4561 tcgcggatgc gtcgcgcctg cgcggcggca ttgccgatgt agctcgccgg cgtcatctcc
     4621 gccaggctgc gcctggcctc ggccgggatc gccaggccgt cgatgaaggc gcgcatctcg
     4681 gcggcaccca ggcgctggcc gcgcgtcagc gctttcagct tctcgtaggg ttccggcacc
     4741 gcgtagcggc gcatcaccgt ctggatcggc tcggccagca cctcccagtt ggcgtcgagg
     4801 tccgccagca ggcgcgtggc gtcgagctcc agcttgccga tgcctttcag cgtggcctcg
     4861 taggcgatca ggctctgcgc gaaggcgccg ccgaggttgc gcagcaccgt ggagtccgtg
     4921 aggtcgcgct gccagcgcga gatcggcagc ttctcgatca ggtggccgaa caccgcgttg
     4981 gcgaggccga ggttgccttc ggcgttctcg aagtcgatcg ggttcacctt gtgcggcatc
     5041 gtcgaggagc ccacttcgcc ggcccgggtg cgctggcgga agtagccgat cgagatgtag
     5101 ccccagacat cgcgcgcgaa gtcgagcagg atcgtgttga agcgcgtcat cgcggcgaac
     5161 agttcggcga ggcagtcgtg cggctcgatc tgcgtggtgt aggggttcca gtcgagcccg
     5221 agcgaggtca cgaagtcgcg tgcgttcgcc tcccagtcca cggcggggta ggccgcgaga
     5281 tgcgcgttgt agttgccgac cgcgccgttg atcttgccga gcaggcgcac gccggcgacc
     5341 tgccggcgct ggcggtgcag gcgataggcc acgttcgcga tttccttgcc catcgtcgag
     5401 ggcgtggcgg gctggccgtg cgtgcgcgag agcagcggga ccgcggccca gtcctcggcc
     5461 atcgcggcca gcgcctcgat gatgcggtcc atcgccggca gcatgacctc ggcgcgcgcc
     5521 gcgttcacca tcagcgcatg gctcaggttg ttgatgtctt ccgaggtgca ggcgaagtgc
     5581 acgaattcca gcccggcggc ggcacccggg atcgcatgga tgcgctcctt gaggaagtac
     5641 tcgaccgcct tgacgtcgtg gttggtcgtt gcctcgatgg ccttgacgcg ctcggcgtcg
     5701 gcgagcgcaa agcccgaggc gatcgcatcc agcgcggccg aggtcgcggt gtcgaaggga
     5761 ccgaactccg gcacgcccgg cagcgcggcg aggcactgca gccagcgcac ctcgaccagc
     5821 acgcggaagc ggatcaggcc gaattcgctg aagatcgggc gcaggctggc cgtcttggcg
     5881 ccgtagcgcc cgtcgagggg ggagatggcg ctgagttcgc tgagctgcat ggtgttccct
     5941 gtttgcggcg cgccgcggcg cgcgtgtcgt ggtggatgat agcgcaagct ttgtggcgtg
     6001 ggttgatggg atggtcccct ccctccttac cggcatcgag tgccagactg gg
//
LOCUS       DAORUQ010000180         6026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15603_length_6026_cov_6.615810, whole genome shotgun sequence.
ACCESSION   DAORUQ010000180 DAORUQ010000000
VERSION     DAORUQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_6026_cov_6.615810"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLW13_13205"
     CDS             complement(<1..467)
                     /locus_tag="PLW13_13205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQY71385.1"
                     /translation="MRNSVPVHCQRVSPAEWSGRVAALAAADARLLTLWGSDERDRGA
                     GELLRAAFLEGGGIVVLELALAGDAPDYPRIDAQFPSAARMQRAVRDLSGIAAQGADA
                     RPWLRHGAWSQHEHPLRRDFVLGTPDPAHYRFVRVQGEGVHEIAVGPVHAGII"
     gene            complement(473..1924)
                     /locus_tag="PLW13_13210"
     CDS             complement(473..1924)
                     /locus_tag="PLW13_13210"
                     /inference="COORDINATES: protein motif:HMM:NF005043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HQY71386.1"
                     /translation="MEMLILLALPLCGAALLALVGARRRAAEVNVAFSLATLVAGGFL
                     TARVIEQGPQLYWNEAFFVDPFNVFLVALTAFVNFTTALFSRRYMRIEERHGRLSASR
                     LRLYHSMYQLFMFTMLVALLTNNMGILWVAMEAATLSTVLLVSLYRTSASLEAAWKYF
                     ILCGVGIAQALFGTILLYFAAERVLGHAGMSALLWTQLDAVKGELEPTVLAIAFAFLL
                     VGYGTKVGLAPLHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVIRCKVLVEGSIRSEL
                     PGQMLMFFGLVSVLVAAFMLWRQKDIKRLFGYSSIEHMGIITFAFGMGGPVANFAALL
                     HMTVHSLTKSAIFFAVGHAVQKAGTQQMDDIRGLLTLSPALGWGLMLGSLAILGMPPF
                     GVFASEFLILTTAMREQPWATPLLLLALGVAFAAVFGRAQSMVFGPPSVAKLPEPPSL
                     LPVFVHLALVLMLGLWIPPFLAGWYRLAAGMIG"
     gene            complement(1932..2603)
                     /locus_tag="PLW13_13215"
     CDS             complement(1932..2603)
                     /locus_tag="PLW13_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71387.1"
                     /translation="MPMQAIPLPEQFIHLLASVVLLLSFAMLAQRRIRTLIHLFALQG
                     AALVCSTLIVAWSSGQHHLYYSAALTLALKVIVLPLILYRLTDRLDLRWDTETGLRIP
                     TTMLVGVVLVVFAFGLAQPISSLAGSVTQSTLGIAIAVVMLAFLMMITRRKAVTQVIG
                     FLAMENGLFFAATSATNGMPMVVEFGIALDVLVGAIILGIFFFQIREQFDSLDLHHLE
                     RLKEE"
     gene            complement(2603..3553)
                     /locus_tag="PLW13_13220"
     CDS             complement(2603..3553)
                     /locus_tag="PLW13_13220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQY71388.1"
                     /translation="MTGAALITQLFAVAVAVLVAPLFLGWVNMCRAWLQNRSAPPLTQ
                     PYRMIRKLLRKDAVIATDASPLFVFAPYVIFGSMVTAAAIIPALALDLPFARAADAIA
                     LVGLFATARVFVALAAIDTGTAFGTLGARREMMLGFLAEPALLMVFFTASLIAGSTEL
                     PVIADMHQYHALAIHPGLAFAAIAFLMVLLAENARIPVDNPATHLELTMIHEAMVLEY
                     SARHLALIEWASALKLFNYACIGFALFVPWGVARDLDSPQVLALALVVLCAKLLVCGA
                     VLALIETLNAKIRIFRAPEFLALAFMLAVLGLLVRLLLQS"
     gene            complement(3550..5553)
                     /gene="hyfB"
                     /locus_tag="PLW13_13225"
     CDS             complement(3550..5553)
                     /gene="hyfB"
                     /locus_tag="PLW13_13225"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HQY71389.1"
                     /translation="MPEPRLALIAALAVALGWLVVGLAGLVRPQSTFVVSRLMFPLGA
                     LGGVLLAIAGLASLAAPPVELVLPLGLPDLPMHLRLDSLSAFFLFLLGSAVAGISVFA
                     AGYFRSGEGTSPGLLCLQYHVFLASMTLVLLANDAYAFMVSWESMALASYFLVITQHR
                     IPEIRAAGFLYLLLAHVGAIAILLCFGVLQGGSWQFTFDAMREAELPVARASLAFGLA
                     VLGFGAKAGLVPLHVWLPEAHPAAPSPVSALMSGVMLKTAVYGVLRVSFDLLPAPLWW
                     WGAVLLALGLFSALYGVVFAAVQTDMKRLLACSSIENLGIVFAGIGLALLFHAEGMGA
                     PAALALAATLYHCLNHAMFKSLLFLATGSVLHATGERNLGRLGGLIRRMPWVAWLALI
                     GTLAIAGLPPLNGFVSEWLLLQAFLLTPDVPQPFMNMLVPLGASLLALTAALAAYVMV
                     KFYGVIFLGQMRERALAEAHDCGRLERLGLLWLAAWCVLLGLLPVAVIGLLDEVGMQL
                     LGASLAHAGSSAFGLVPVSAERAAYRPIVFLVGIALVLIVTVLVAKRLYHGRTRRAPP
                     WDCGFAAQDARMQDTAEGFGQPIRHIFDAFFSMRRELPSPFDEHPVYRVTVEDRFWGL
                     LYQPLARAVKRAAAQVGRLQTGRISVYLAYSFVTLIVLLVIVL"
     gene            5706..5918
                     /locus_tag="PLW13_13230"
     CDS             5706..5918
                     /locus_tag="PLW13_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQY71390.1"
                     /translation="MEQRTARLTVLIDPGKKAVFEHLCAREDQTPSQVVRRLVRDYIE
                     SRLGHPWRPGDTVESALAQNLANEAN"
BASE COUNT         1167 a         2046 c         2069 g          744 t
ORIGIN      
        1 tcgatgatgc cggcatgcac cggcccgacc gcgatctcgt gcacgccctc gccctgtacg
       61 cgcacgaagc ggtaatgcgc gggatcgggc gtaccgagga cgaagtcccg ccgcagcgga
      121 tgttcgtgct gcgaccacgc cccgtggcgc aaccagggcc gcgcatcggc gccctgcgcg
      181 gcgatgcccg acaggtcgcg gaccgcacgc tgcatccgcg cggcggaggg aaactgcgca
      241 tcgatgcgcg gatagtccgg cgcatcaccc gccagcgcga gctcgagcac cacgatgccg
      301 ccgccctcga gaaaggccgc gcgcagcagc tcgccggcgc cgcggtcgcg ttcgtcgcta
      361 ccccacagcg tgagcaggcg cgcatccgcc gccgccagcg cggcgacgcg cccggaccat
      421 tctgcagggg atacgcgctg gcaatgcacg ggcaccgagt tgcgcatcgc gttcacccga
      481 tcatcccggc ggcgaggcgg taccagccgg cgagaaacgg cggtatccac aggccgagca
      541 tcagcaccag cgcgaggtgc acgaacaccg gcagcagcga cggcggctcg ggcagcttcg
      601 cgacgctcgg gggaccgaac accatcgatt gcgcgcggcc gaacaccgcg gcgaaggcga
      661 cgccgagcgc gagcagcagc agcggagtcg cccacggctg ttcgcgcatc gcggtggtga
      721 ggatcaggaa ctcgctcgcg aacacgccga agggcggcat gccgagtatc gcgagcgatc
      781 cgagcatcag gccccagccg agtgcgggcg agagcgtcaa caggccgcgg atgtcgtcca
      841 tctgttgggt gcctgccttc tgcaccgcat gccccacggc gaagaagatc gcgctcttgg
      901 tcagcgaatg caccgtcatg tgcagcagcg cggcgaagtt tgccaccggc ccgcccatgc
      961 cgaaggcgaa ggtgatgatg cccatatgct ctatcgacga gtagccgaag agccgcttga
     1021 tgtccttctg ccgccacagc atgaaagccg cgaccagcac cgataccagc ccgaagaaca
     1081 tcagcatctg ccccgggagc tccgagcgta tcgagccctc gaccagcacc ttgcagcgga
     1141 tcaccgcgta cagcgccacg ttcagcagca agcccgagag caccgccgag accggtgtgg
     1201 gaccctcggc atgcgcgtcg ggcagccagt tgtgcagcgg cgcgagcccg accttggtgc
     1261 cgtagccgac cagcaggaac gcgaaggcga tcgcgagcac ggtgggctcg agctcgccct
     1321 tcaccgcgtc gagctgcgtc cacagcagcg cgctcatgcc ggcgtgcccg agcacgcgct
     1381 cggccgcgaa gtacagcagg atggtgccga acagcgcctg cgcgatgccg acaccgcaca
     1441 ggatgaagta cttccacgcc gcttccaggc tggccgaagt gcggtacagc gagaccagca
     1501 gcaccgtcga cagcgtcgcc gcttccatcg cgacccacag gatgcccatg ttgttggtga
     1561 gcagcgccac cagcatcgtg aacatgaaca gctggtacat gctgtggtac aggcgcaggc
     1621 gcgacgccga gagccggccg tggcgctcct cgatgcgcat gtagcggcgc gagaacagcg
     1681 cggtcgtgaa gttcacgaag gcggtgagtg cgaccaggaa cacgttgaag ggatcgacga
     1741 agaaggcctc gttccagtac agctgcgggc cctgctcgat cacgcgcgcg gtcaggaagc
     1801 cgccggcgac cagtgtcgcg aggctgaacg ccacgttcac ttccgcggcg cggcggcgcg
     1861 cccccacgag cgcgagcagc gcggcgccgc agagcggcag ggcgagcagt atcagcattt
     1921 ccatgggcgg ctcattcctc cttcagccgt tccaggtggt gcaggtcgag gctgtcgaac
     1981 tgctcgcgga tctggaagaa gaagatgccg aggatgatcg ccccgaccag cacgtcgagc
     2041 gcgatgccga actccacgac catcggcatg ccgttggtcg cgctggtggc ggcgaagaac
     2101 aggccgtttt ccatagcgag gaagccgatg acctgcgtga cggccttgcg ccgcgtgatc
     2161 atcatcagga aggccagcat caccaccgcg atcgcgatgc ccagcgtgct ctgcgtcacg
     2221 ctgccggcga ggctggatat gggctgcgcg aggccgaagg cgaataccac cagcaccacg
     2281 ccgaccagca tggtggtggg gatgcgcagg ccggtctcgg tgtcccagcg caggtccagc
     2341 cggtcggtca gccggtacag gatcagcggc agcacgatca ccttgagcgc cagcgtgagt
     2401 gcggcggagt agtacaggtg gtgctgaccg ctggaccagg ccacgatcag ggtcgagcac
     2461 accagtgccg cgccctgcag cgcgaacagg tggatcagcg tgcggatgcg ccgctgcgcg
     2521 agcatcgcga aggacagcag cagcaccacc gaggcgagca ggtggatgaa ctgttccggc
     2581 aacgggattg cttgcatcgg catcaactct gcagcagcag gcgcacgagc aggccgagca
     2641 cggccagcat gaaggcgagc gcgaggaatt ccggcgcgcg gaagatgcgg atcttcgcgt
     2701 tcagggtctc gatcagcgcc agcaccgcgc cgcagacgag cagcttcgcg cacagcacca
     2761 ccagcgcgag cgccagcacc tgcggcgaat cgagatcgcg cgcgacaccc cacggcacga
     2821 acagcgcaaa gccgatgcag gcgtagttga agagcttcag cgccgatgcc cactcgatca
     2881 gcgccaggtg gcgcgcggaa tactcgagca ccatcgcctc gtggatcatc gtgagctcga
     2941 ggtgcgtggc cggattgtcg accgggatac gggcgttctc ggcgagcagc accatcagga
     3001 aggcgatggc ggcgaaggcg agccccgggt ggatggcgag cgcatggtac tggtgcatgt
     3061 ccgcgatcac cggcaactcg gtcgaaccgg cgatcagcga ggcggtgaag aacaccatca
     3121 gcagcgccgg ctcggcgagg aagccgagca tcatctcgcg ccgcgcgccg agcgtgccga
     3181 aggcggtgcc ggtgtcgatc gcggccagcg cgacgaagac gcgcgcggtg gcgaagagcc
     3241 cgaccagcgc gatcgcgtcc gcggcgcgcg cgaacggcag gtcgagcgcc agcgccggga
     3301 tgatcgcggc ggccgtcacc atcgagccga agatcacgta gggcgcgaac acgaacagcg
     3361 gcgaggcgtc cgtggcgatc accgcgtcct tgcgcaacag cttgcggatc atccggtagg
     3421 gctgcgtgag cggcggggcg gagcggttct gcagccacgc ccggcacatg ttgacccagc
     3481 cgaggaacag cggcgcgact agcacggcca ccgccaccgc gaagagctgg gtgatcagcg
     3541 ccgcgcccgt catagcacga tcaccagcag cacgatcagc gtgacgaagc tgtatgccag
     3601 gtacaccgag atgcggcccg tctgcagccg gcccacctgg gccgcggcgc gcttcaccgc
     3661 ccgcgccagc ggttggtaca gcaggcccca gaagcggtcc tcgacggtga cgcggtagac
     3721 gggatgctcg tcgaagggcg agggcagctc gcggcgcatg ctgaagaagg cgtcgaagat
     3781 gtggcggatc ggctgcccga agccctcggc ggtgtcctgc atgcgcgcat cctgggccgc
     3841 gaagccgcaa tcccacggcg gtgcgcgacg cgtgcggccg tggtacaggc gcttggcgac
     3901 cagcacggtg acgatcagca ccagcgcgat gccgaccagg aacacgatcg ggcggtaggc
     3961 cgcgcgctcc gccgacaccg gcaccaggcc gaaggcgctg gaaccggcat gcgcgagcga
     4021 ggcaccgagc agctgcatgc cgacctcgtc gagcaggccg atcacggcca ccggcagcag
     4081 cccgagcagc acgcaccagg ccgccagcca cagcagcccc aggcgctcga ggcggccgca
     4141 gtcgtgcgcc tcggcgagcg cgcgctcgcg catctgcccg aggaagatca cgccgtagaa
     4201 cttcaccatc acgtaggcgg ccagcgccgc ggtgagcgcc agcagcgagg cgccgagcgg
     4261 caccagcatg ttcatgaacg gttgcggcac atcgggcgtg agcaggaatg cctgcagcag
     4321 cagccactcg gatacaaagc cgttcagcgg cggcagcccc gcgatcgcca gcgtcccgat
     4381 cagcgccagc cacgccaccc acggcatgcg ccggatcagg ccgccgagcc tgccgaggtt
     4441 gcgctccccc gttgcgtgca gcaccgagcc ggtggcgagg aacagcaggc tcttgaacat
     4501 cgcgtggttc aggcagtggt agagcgttgc cgcgagcgcc agcgccgcgg gcgcgcccat
     4561 gccctcggcg tggaacagca gcgccagccc gatgcccgcg aacacgatgc cgagattctc
     4621 gatggacgag caggcgagca gccgcttcat gtcggtttgc accgcggcga acaccacgcc
     4681 gtagagcgca ctgaacaggc cgagcgccag cagtaccgcg ccccaccacc acagcggcgc
     4741 cggcagcagg tcgaagctca cgcgcaacac gccgtagacg gcggtcttca gcatcacgcc
     4801 gctcatcagc gcggaaaccg gcgatggcgc ggccggatgc gcctcgggca gccacacgtg
     4861 cagcggcacc agcccggcct tggcgccgaa gccgagcacc gccaggccga aggcgaggct
     4921 cgcgcgcgcc acgggtagct cggcctcgcg catcgcgtcg aaggtgaact gccagctgcc
     4981 gccctgcagc acgccgaagc agagcaggat cgcgatcgcc ccgacgtgcg cgagcagcag
     5041 gtagaggaaa ccggcggcgc ggatctcggg gatgcggtgc tgggtgatga cgaggaaata
     5101 cgaggccagc gccatcgatt cccagctgac catgaacgcg taggcatcgt tggcgagcag
     5161 caccagcgtc atgctggcga ggaagacgtg gtactgcagg cacagcagcc cgggcgacgt
     5221 gccttcgccg gaacggaaat agccggccgc gaacacgctg atcccggcca cggcgctgcc
     5281 aagcaggaac aggaagaagg cggacagcga atcgagtcgc aggtgcatcg gcagatcggg
     5341 aagcccgagc ggcagcacca gttcgaccgg cggcgcggcg agcgaagcca acccggcgat
     5401 ggcgagcagc acgcctccca gcgcgccgag cggaaacatc aggcggctga cgacaaaggt
     5461 actttgcggg cgcaccagcc ccgccaatcc cacgaccagc cagccgagcg caaccgccag
     5521 cgcggcgatc agcgccagcc ttggctcagg cacccgagcc gtccgcgccg cgacaagcct
     5581 gcatccggtc acccgtcatc acaccacccc tgaaagaagc atagcttctt gccggattcg
     5641 atgagtgcta atctaatgtg cgtgtgatgt gtcgtcaacg caactttggt acacaccgta
     5701 tgagcatgga acagcgcacc gccaggctga ccgtcctgat cgatccgggc aaaaaggccg
     5761 ttttcgaaca cctgtgcgcg cgcgaggacc agacgccctc gcaagtggtg cgccgcctgg
     5821 tgcgcgacta catcgaaagc cgcctcggcc atccctggcg cccgggcgac acggtggagt
     5881 cggcactcgc gcagaacctt gcgaacgagg cgaactgagc gcccgtgtgg gtcgccattc
     5941 atggcgacag ctgcgttcgg gaacgcacaa agtcggcatg aatgccgtat atggtcccct
     6001 ccctgtttgc aaggtttcca gatctg
//
LOCUS       DAORUQ010000181         5994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15710_length_5994_cov_5.908907, whole genome shotgun sequence.
ACCESSION   DAORUQ010000181 DAORUQ010000000
VERSION     DAORUQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15710_length_5994_cov_5.908907"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1619)
                     /locus_tag="PLW13_13235"
     CDS             complement(48..1619)
                     /locus_tag="PLW13_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HQY71391.1"
                     /translation="MADGTALPLVMPELLAWAAARHSERIALRDGEVTLDFITLAARA
                     RDASRAFLAAGIRRGDRFAIWAPNRYEWILAALGAQNIGAILVPLNTRMKGAEAAYIL
                     ERSGARLLFTVGEFAGNRYPEMLTGIALPALERTVLFGEAAGGPGGWEDFVAAGRAVS
                     DAEVRAAETAVMPDDTLDLMFTSGTTGKPKGVMTGHAQNIRSFDTWGRTVGLGADDNY
                     LIINPFFHSFGYKAGWLAAIIRGARILPVASFDVDQVLRRIQFERVSMLPGPPTIYQS
                     LLAHPERQSYDLSSLRLAVTGAAAVPVDLIRRMRSELGFRTVLTAYGLTETCGVVTIT
                     GAEDEPELIATTAGRAMPGVEVICVNSAGEPVPAGEPGEVCVRGFNVMQGYFDDPEAT
                     AEAITPDGWLKTGDIGVLNEAGYLRITDRIKDMFIVGGFNCYPAEIENLLCSLGGVAQ
                     AAVIGVPDERMGEVAKAFVVRRAGSTLTEQEVIDWCRRNMANYKVPRSVAFVDALPLN
                     AAGKVLKTALRERSG"
     gene            1875..3110
                     /locus_tag="PLW13_13240"
     CDS             1875..3110
                     /locus_tag="PLW13_13240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY71392.1"
                     /translation="MSATSDPAQTAYLAQRPGVNAHWRRRFALALLCVAAGLGLSHFL
                     SGSLRADGALAPPERFVLVFAYLGCIALLALLADPGPLTRRARVRTLLDDAWMALLMP
                     GAGALATLDATKGDLTGYLLVCLPVAIFHSTAAARAVALQLCAFGCAAPGIVLYGGLD
                     NGATALWLSVVGACTAVFAYIAVAIETGRHNAFITAYELELRSAALTEANARLESNNR
                     KLELRTAELARLNADLEESSAALETANKRLEELAGTDPLTGVANRRQLFEAIAREFGR
                     ARRYGSELALAVIDLDDFGPINKRHGVIAGDEVLAEFAAALRAQTRAVDLVARYGGEE
                     FVVLMPGCAAGAAAQLLERVRASIAGVPLGSRRITLTFSAGVAELAADDTDPVNVVHR
                     ADAALRRAKAGGKNRVERS"
     gene            3107..4483
                     /locus_tag="PLW13_13245"
     CDS             3107..4483
                     /locus_tag="PLW13_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQY71393.1"
                     /translation="MNAARAPQGGDAAVSSTAILALALPSSTMFLLATLVGIAMIRFA
                     AALGPDAVAAVNAGARLYNVFIALAAGINAGALALIANAWGAGRRDEAERYLALSLAV
                     AALLGAAITLLTWLGAGPLLGLFDLDPGAHTESVSYTRWLALFYAPIALYLVLASGLR
                     AAGDARTPMLFALLVNGVSLGLAWRWSIAPPFGLQPHVRYIALGLGLGNVAGVVAALA
                     LWRARRLALAPARRDAGSRQRLKALWQVGYPAALEQGLLQLGIIAFLWVVAQYGTAAF
                     TAYGTSISLLSLAMVIGFGFSVAVSVMVGQQLGAGSPANARLVALRALRLTIVTLSVP
                     GLILACYAHPVALWLTGDEGIAAHAVPILYAFTLALPMLAVEFCLGGALRGAGDTRFP
                     LLNVLAGLIVVRFGLALLFYYQGLGVNWIYATLIADYAVKNALLVWRFRSGRWMKLLR
                     AETPLARP"
     gene            complement(4548..5363)
                     /locus_tag="PLW13_13250"
     CDS             complement(4548..5363)
                     /locus_tag="PLW13_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQY71394.1"
                     /translation="MTYENPRIPEGINVARESVPAEFLRLLAGLLLVVVVAALVLHLA
                     GGWLARQVPFALERSWVGERLLVPPGIGTDAADAQAQERVQALARELAAGMGLPQGMT
                     VDVHLVREEVPNAFATLGGHVLVTDGLYRRLPSENALAMVLAHELAHIRARDPIAAAG
                     GGAALTLLMLALGGDAEALAPQLARLVQLGYSRQAEARADEAAIAALLGHYGHAGGAA
                     GLFEALADYDGAGRELPALLATHPADAARIARLQQAARGWDATRQPLVALPAL"
     gene            complement(5360..5830)
                     /locus_tag="PLW13_13255"
     CDS             complement(5360..5830)
                     /locus_tag="PLW13_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HQY71395.1"
                     /translation="MEAMLQLLVFAALLLAGLLFGRAAERRHYRDIVAREEALGDILV
                     FSERRIPDFAAPPRTTLVVGSVVIAEDYFKRIAASLKSLVGGRLTAYESLMDRGRREA
                     VLRMKEEARGLGADMVFNVRFETASMNDSAGGRQAMFSAEFIAYGTALIPSRAP"
BASE COUNT          881 a         2185 c         2024 g          904 t
ORIGIN      
        1 gccgcaaagc ggcgggcgca gcaggcgggc gaaagattca caggctctca gcccgagcgc
       61 tcgcgcagcg cggtcttcaa taccttgccc gcggcgttga gcggcagcgc gtcgacgaag
      121 gcgacgctgc gcggcacctt gtagttcgcc atgttgcgcc ggcaccagtc gatcacttcc
      181 tgctcggtga gcgtgctgcc ggcacggcgc acgacgaagg ccttcgcgac ctcgcccatg
      241 cgctcgtcgg gcacaccgat caccgcggcc tgcgcgacac cgccgaggga acacaacagg
      301 ttctcgatct cggccggata gcagttgaag ccgccgacga tgaacatgtc cttgatgcgg
      361 tcggtgatgc gcagatagcc cgcctcgttc agcacgccga tgtcgccggt cttcagccag
      421 ccgtccggcg tgatcgcctc tgcggttgcc tcgggatcgt cgaaatagcc ctgcatgacg
      481 ttgaagccgc gcacgcagac ctcgccgggc tcacccgccg gcaccggctc tccggcgctg
      541 ttcacgcaga tcacctccac gccgggcatc gcgcgccccg cggtggtcgc gatcagctcg
      601 ggctcgtcct cggcgccggt gatcgtgacc acgccacagg tctcggtgag gccataggcc
      661 gtcaacacgg tgcgaaaacc cagttcgctg cgcatgcgcc ggatcaggtc gaccggaacc
      721 gccgccgcgc cggtgaccgc gagccggagc gaggacaggt cgtaactctg gcgctcggga
      781 tgcgcgagca gcgactggta gatcgtgggc ggcccgggca gcatgctcac gcgctcgaac
      841 tggatgcgcc gcagcacctg atccacgtcg aagctggcca ccggcaggat gcgcgcgccg
      901 cggatgatcg cggccagcca cccggccttg tagccgaagg aatggaagaa ggggttgatg
      961 atcaggtagt tgtcgtcggc gccgagccct acggtgcggc cccaggtgtc gaaggaacgg
     1021 atgttctgcg catgcccggt catcacgccc ttgggcttgc cggtggtgcc ggaggtgaac
     1081 atcaggtcga gcgtgtcgtc cggcatgacc gccgtctcgg ccgcccgcac ctcggcatcg
     1141 ctcacggcgc ggcccgcggc cacgaagtct tcccatccgc ccgggccgcc ggcggcctcg
     1201 ccgaacagca cggtgcgctc gagcgccggc agcgcgatac ctgtcagcat ttccgggtag
     1261 cggttacccg cgaactcgcc gaccgtgaac agcagtcgcg cgccgctgcg ctcgaggatg
     1321 tacgccgcct cggcgccctt catgcgcgtg ttgagcggca ccaggatcgc gccgatgttc
     1381 tgcgcgccaa gggccgccag gatccattcg tagcggttgg gcgcccagat cgcgaaacgg
     1441 tcgccgcgac ggataccggc ggcgaggaaa gcccgggacg cgtcgcgggc ccgcgccgcc
     1501 agcgtgatga aatccagcgt gacctcaccg tcacgcagcg cgatgcgctc actgtggcgc
     1561 gcagccgccc atgccagcag ctcgggcatg accagtggca aagccgttcc gtccgccatt
     1621 cgagcctccc gctagaaatc gcacttctat ttgtataaat aaacgcaaat aatattcttc
     1681 ttcccctcgg gacacaataa ttcgtaatga aagcgatgaa tttctgcgca tttggatggc
     1741 atcaccagtc gtcgggccgc gttccgggcc aattcgcccc gcgaaccggc gctgcgccgg
     1801 gccgggccgc agcacgacgt ctatcctgaa tgagcctcgc ggggcccata cccagccttt
     1861 catcgagccc agagatgtcc gcaaccagcg acccggccca gaccgcctac ctcgcgcagc
     1921 gcccgggcgt gaacgcccac tggcgccggc gattcgccct ggcgttgctg tgcgtcgcgg
     1981 cggggctcgg cctgtcgcac ttcctgagcg gctcgctgcg cgccgatggc gcgctcgcgc
     2041 cacccgaacg cttcgtcctg gtcttcgcct acctgggatg cattgccctg ctcgcactgc
     2101 ttgcggaccc gggaccgctc acgcgcaggg cgcgcgtgcg cacgctgctc gacgacgcct
     2161 ggatggcgct gctgatgccg ggcgcgggcg ccctggcgac gctggatgca accaagggcg
     2221 acctcaccgg ctacctgctg gtctgcctgc cggttgcgat attccacagc accgccgctg
     2281 cgcgcgctgt cgccctgcaa ctatgcgcat ttggctgcgc cgcacccggc atcgtgctgt
     2341 acggcgggct ggacaatggc gccaccgccc tgtggctgtc ggtggtcggc gcctgcaccg
     2401 cggtgttcgc ttatatcgcg gtggcgatcg aaaccggtcg acacaacgct ttcatcaccg
     2461 cctacgagct cgagctgcgc tcggcggcac tgaccgaggc caacgccaga ctcgagagca
     2521 acaaccgcaa gctggagctg cgcaccgccg aactggcgcg cctcaacgcc gacctcgagg
     2581 aaagcagcgc agcgctggaa accgccaaca agcgcctcga ggagctcgcg ggcaccgatc
     2641 cgctgaccgg cgtggcgaac cgccgccagc tcttcgaggc gatcgcgcga gagttcggcc
     2701 gcgcgcgacg ctacggcagc gagctcgcac tggcggtcat cgatctggac gatttcgggc
     2761 ccatcaacaa gcggcacggc gtcatcgcgg gcgacgaggt gctcgcggaa ttcgccgccg
     2821 cgctgcgcgc gcagacgcgc gcggtcgacc tggtggcccg ctacgggggc gaggagttcg
     2881 tggtgctcat gcccggttgc gcggccggcg cggccgcgca gctgctggag cgcgtgcgcg
     2941 cgtccatcgc cggagtgccg ctcggctcgc ggcgcatcac gctcactttc agtgccggcg
     3001 tggcggaact ggccgccgac gacaccgacc cggtcaacgt ggtgcatcgc gccgacgcgg
     3061 cactgcgccg cgccaaggcc gggggcaaga accgcgtgga acgcagttga acgcggcacg
     3121 agcgccgcag ggcggcgacg ccgccgtatc gtccacggcc atcctcgcgc tggcgcttcc
     3181 ttcgagcacg atgttcctgc tcgccacgct ggtcggcatt gcgatgatcc gcttcgcggc
     3241 ggcgctggga cccgatgcgg tagcggcggt gaacgcggga gcgcgactct acaacgtgtt
     3301 catcgcgctc gcggccggca tcaacgccgg ggcgctggcg ctgatcgcca atgcctgggg
     3361 cgcgggcagg cgcgacgagg ccgagcgtta cctcgcgctg tcgctcgcgg tcgccgcgct
     3421 gctcggcgcc gccatcacgc tgctcacctg gctcggcgcc gggccgctgc tcgggctgtt
     3481 cgacctcgac ccgggcgcgc acacggaatc ggtgagctac acccgctggc tggcgctgtt
     3541 ctacgcgccg atcgcgctct acctggtgct cgcctcgggc ttgcgcgcgg ccggcgacgc
     3601 gcgcacaccg atgctgttcg ccctgctggt gaacggcgtg tcgctgggcc tcgcgtggcg
     3661 ctggagcatc gccccgccgt tcgggctgca accgcacgtg cgctacatcg cgctcgggct
     3721 gggactgggc aacgtggcgg gtgtggtggc ggcgctcgcc ctgtggcgag cccggcgcct
     3781 ggcgctcgcc ccggcgcgcc gcgatgcggg cagccgccag cgcctgaagg cactgtggca
     3841 ggtgggctac ccggccgcgc tcgagcaggg actgctgcaa ctgggcatca tcgcgttcct
     3901 gtgggtggtc gcgcagtacg gcaccgcggc attcacggcc tacggcacca gcatcagcct
     3961 gctgtcgctc gcgatggtga tcggcttcgg cttctcggtc gcggtgtcgg tgatggtcgg
     4021 gcaacaactc ggcgcaggct cgcccgccaa tgcgcgcctg gtggcactgc gcgcgctgcg
     4081 cctgaccatc gtgacgctga gcgttcccgg attgatactc gcgtgttacg cgcatcccgt
     4141 cgcgctgtgg ctcaccggcg atgagggcat cgccgcccac gcggtgccga tcctctacgc
     4201 gttcacgctc gcgttgccga tgctggcggt ggaattctgc ctcggcggcg cgctgcgcgg
     4261 ggccggcgac acgcgctttc cgctgctgaa cgtgctggcc ggcctgatcg tggtgcgttt
     4321 cggcctggcc ctgcttttct actaccaggg cctcggggtg aactggatct acgccacgct
     4381 gatcgccgat tacgcggtca agaacgcgct gctggtgtgg cgtttccgtt ccgggcgctg
     4441 gatgaaactg ctgcgcgcgg aaacgccact cgctcgcccg taggtcctca ttcatgcgga
     4501 cattgtccgg ctgaagccgg aaccacgaga cccgcagcgc acaggaatca cagcgcgggc
     4561 agggcaacca gcggctgccg ggtcgcgtcc cagccgcgcg cggcctgctg caggcgcgcg
     4621 atgcgcgcgg catcggccgg gtgcgtggcc agcaacgcgg gcagttcacg gcccgccccg
     4681 tcgtagtccg ccagcgcctc gaacagcccc gccgcgccac cggcgtggcc gtagtggccc
     4741 agcagcgccg cgatcgcggc ctcgtcggcg cgcgcttcgg cctggcgcga atacccgagt
     4801 tgcaccaggc gcgcgagctg cggcgccagc gcttcggcat cgccacccag cgccagcatc
     4861 agcagcgtga gtgccgcacc gccgccggcc gcggcaatcg ggtcgcgcgc gcgaatgtgc
     4921 gcaagctcgt gcgccagcac catcgccagg gcgttctcgc tgggcaggcg ccggtagagc
     4981 ccgtcggtga cgagcacatg cccgcccagc gtcgcgaagg cgttcggcac ctcctcgcgc
     5041 accaggtgca cgtccaccgt catgccctgc ggcagcccca tgccggcggc gagctcgcgc
     5101 gcgagcgcct gcacgcgctc ctgcgcctgc gcgtcggcgg catcggtgcc gattcccggc
     5161 ggcaccagca gccgctcgcc gacccaggaa cgctcgagcg cgaacgggac ctgccgcgcc
     5221 agccagccgc cggccaggtg cagcaccagc gccgccacca cgaccacgag cagcaagccg
     5281 gcaagcaggc gcaggaactc cgcgggcacg ctctcgcgcg ccacgttgat gccttcggga
     5341 atgcgcgggt tctcgtatgt cacggcgcgc gtgacgggat cagcgccgtg ccataggcga
     5401 tgaattccgc cgagaacatc gcctgccgtc cgccggcgct gtcattcatc gacgcggtct
     5461 cgaagcgcac gttgaatacc atgtcggccc cgaggccccg cgcctcctcc ttcatgcgca
     5521 gcaccgcctc gcgccgaccg cgatccatca acgactcgta ggccgtgagg cgcccgccga
     5581 ccaggctctt caggctcgcc gcgatacgct tgaaatagtc ctccgcgatc accaccgagc
     5641 ccacgaccag cgtcgtgcgc gggggcgccg caaagtcggg gatgcggcgc tcgctgaaca
     5701 cgagaatgtc gcccagcgcc tcctcgcgcg cgacgatgtc acggtagtgg cgccgctccg
     5761 cggcgcgccc gaacagcagc cccgcgagca gcagggcagc gaagaccagc aactgcagca
     5821 ttgcctccat cgtcggctac ccgatcacca ccgcggtgcc gtaggccatc acctccgcgg
     5881 cccccgaggc gatgttcgag gtcgagaagc gcacgttcac caccgcgttc gcgccgaccg
     5941 cctcggcctg ctgcaccatg cgcgccaccg cctccttgcg cgactccacc agca
//
LOCUS       DAORUQ010000182         5984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15749_length_5984_cov_4.884804, whole genome shotgun sequence.
ACCESSION   DAORUQ010000182 DAORUQ010000000
VERSION     DAORUQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15749_length_5984_cov_4.884804"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="motD"
                     /locus_tag="PLW13_13260"
     CDS             complement(<1..770)
                     /gene="motD"
                     /locus_tag="PLW13_13260"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HQY71396.1"
                     /translation="MARRIIREDTVSHERWMVSYADFLTLLLAFFVVMYAVSQRNEDK
                     FRVLSHTLSEAFNMPERSINPIQVGEPVTAVNPGNVPDAIESPEQLPGSGRIEEDEPK
                     ATTSEPQLPEEFRRISRRVEEQFADLVARDLISVQGNEEWLEIELKSSLLFASGEATA
                     SAEARPIMQEMAAVVRDSELPIRVEGFTDDRPIATGIYPSNWELSSARAATIVKLLTE
                     FGVGPTRLAAIGYGEFQPVAGNDSEEGRAANRRVVLMLS"
     gene            complement(787..1527)
                     /locus_tag="PLW13_13265"
     CDS             complement(787..1527)
                     /locus_tag="PLW13_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003257303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HQY71397.1"
                     /translation="MDVLSIIGIVLAFTAVVGGNLLEGGELSALANLPAALLVVGGTV
                     GAGLLQMPLAGLRRALAMMRWVFVPPRHGFGEGVKQVVRWATTARREGLLGLEAVAEL
                     EPDAFARRGLQLLVDGNEPEAIRSVLESESGLREQRDVDAAKFYESMGGYAPTIGIIG
                     AVVGLIHVMGNLADPANVGPGIAVAFVATIYGVALANLFFLPIAAKLRSCVREAAQYR
                     EMIIEGIIAIADGENPRAIELRLNGYLE"
     gene            complement(1559..2266)
                     /locus_tag="PLW13_13270"
     CDS             complement(1559..2266)
                     /locus_tag="PLW13_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HQY71398.1"
                     /translation="MYAKTGARAQPQELVEQHGPLVRRIAHHLLARLPSSVSVEDLIQ
                     SGMIGLLEAAASYDATRGASFETFAGIRIRGAMIDEVRRGDWSPRSVHRNARRVAAGI
                     RNVELRSGGDAGDAAVARELGVEIAEYHAMLQDTLGSRLFSLDELMGEEHGERSGFAS
                     AALNPAEQAARERLAGAIGAEIARLPERERLVLSLYYDDELNLKEIGLVLEVSESRVS
                     QILSQATKRLRARLQDW"
     gene            complement(2291..3127)
                     /locus_tag="PLW13_13275"
     CDS             complement(2291..3127)
                     /locus_tag="PLW13_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HQY71399.1"
                     /translation="MNSAHGAGRPVQVVAVTGGKGGVGKTSVSINLAIALAARGRRVV
                     LLDADLGLANVDVLLGLRPKRNLADVLAGGCGLEDIMLDGPGGIRIIPASSGTQSMAS
                     LSRLEHAGLIHAFSAIGSGMDVLLIDTAAGIADSVLSFVRAAQECLVVVCDEPSSIAD
                     AYALMKLLHRDHGMQHFRLLGNMVRSAQEGASLYHKLAQVCDRFLDITLLDAGHIPYD
                     DMVRKCIQRQRPVLLGAPQSRAAAAFRELAARVERWPIPAGPSGHLQFFVEQLLDHAA
                     AG"
     gene            complement(3153..>3929)
                     /gene="flhF"
                     /locus_tag="PLW13_13280"
     CDS             complement(3153..>3929)
                     /gene="flhF"
                     /locus_tag="PLW13_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HQY71400.1"
                     /translation="VDAGTAWDAAWRATQARLVEGLGTVGDVVASGGVWAFVGPTGAG
                     KTTTICKLAVRHVLEHGPGGLALLSMDAARLGGADMLRAVARLLGVPFHAADSGESIG
                     EALRRVPANALVLVDTAGVSRRAHVRSRQLDELGTLREGVRTLLVLAANAQLSWLHAA
                     LADYAACVPVGAVVTKLDETASLGELIGALRRERLPVAYSTDGPEIPDDLRVADAAEL
                     VAQALALEPGEEAAREGTPAAAGIGHGAPDAAGVNAARIA"
     assembly_gap    3930..4029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4030..4636)
                     /locus_tag="PLW13_13285"
     CDS             complement(<4030..4636)
                     /locus_tag="PLW13_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71401.1"
                     /translation="MELKRIIGPDVKTALRLVREQLGPDAMILSNRRVAGGVEIVAAP
                     EAPPAPALPASRTSAGEPMLRAFDLAAGAAPVAPRPRPSAPERAFARVEDPVPAAAGA
                     APTAAPAAFAVASPTAGEGPTLAPQWLEMQSELRSMRTLLEERLTALRSERTVDGPGT
                     EGRIWRRLTRMGLPNELVRELVAKVDAGTAWDAAWRATQARL"
     gene            complement(4686..>5984)
                     /locus_tag="PLW13_13290"
     CDS             complement(4686..>5984)
                     /locus_tag="PLW13_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HQY71402.1"
                     /translation="VTTSNDMGDQILGQMFDSPRALAVAAALLAIMGLVPGMPHVAFI
                     GLAALAGAGAWLIRRRRREEASGPAAASELARVAPAPAPELGWDDVAPVDVIGLEVGY
                     RLIPMVDKAQGGALLGRIKGVRKKLSQEMGFLVPPVHIRDNLELLPNAYRILLMGVAM
                     GEAEVFPERELAINPGQVFGRLDGLECRDPAFGLDALWIERNQHERAQTLGYTVVDAS
                     TVVATHLNQVLLEHIDELIGHEEVEQLLQKLARMSPKLAEELVPGTLTINQLLGVLKS
                     LLGEGVPIRDIRSIAEAIAAQAVRSKDPAALSAAARVALGRTIFQQISGNAASLPVIT
                     LEPQMEQVLMKSLQRGGAAGAEADDIVLEPSLAERLQRAMNEAAQRQEVQGKAPVLLV
                     PAPLRAVLARFMRHTNRATRVISYQEIPGNKQVTIEATVG"
BASE COUNT          832 a         2221 c         1962 g          869 t
ORIGIN      
        1 gagagcatga gcaccacgcg ccggttggcc gcgcgtccct cctcgctgtc gttgcccgcc
       61 acgggctgga actcaccata cccgatggcg gcaaggcggg tgggcccgac accgaattct
      121 gtgagcagct tcacgatggt cgccgcgcgc gccgaggaca actcccagtt cgacgggtag
      181 atgccggtgg cgatgggccg gtcgtcggta aagccctcga cgcggatcgg cagttcgctg
      241 tcgcgcacca ccgccgccat ctcctgcatg atcggacgcg cctcggcgct cgcggtggcc
      301 tcgccgctgg cgaacagcag gctggacttg agctctatct cgagccattc ctcgttgccc
      361 tgcaccgaga tcaggtcgcg cgccaccagg tccgcgaact gctcctcgac gcgccgcgag
      421 atgcggcgga attcctccgg cagttgcggc tccgaggtgg tcgccttcgg ctcgtcctcc
      481 tcgatgcgtc cggagccggg cagctgctcc ggcgactcga tcgcatcggg cacgttgccc
      541 ggattcaccg cggtcaccgg ctcgccgacc tggatcggat tgatgctgcg ctcgggcatg
      601 ttgaaggctt cgctcagcgt gtgcgagagc acgcggaact tgtcctcgtt gcgctgcgac
      661 accgcgtaca tcaccacgaa gaaggcgagc agcagcgtga gaaagtccgc gtaggaaacc
      721 atccagcgct catggctgac cgtgtcctcc cggatgatcc gcctggccat cgccgcctcg
      781 ccccgcctac tcgaggtagc cgttcaggcg cagctcgatc gcgcgcggat tctcgccgtc
      841 ggcgatcgcg atgatgccct cgatgatcat ttcgcggtac tgcgccgcct cgcgcacgca
      901 actgcgcagc ttggcggcga tgggcaggaa gaacaggttc gccagcgcca cgccgtagat
      961 cgtcgcgacg aaggccaccg cgatgcccgg ccccacgttt gccggatccg cgagattgcc
     1021 catcacgtgg atcaggccga ccacggcgcc gatgatgccg atcgtgggcg catagccgcc
     1081 catgctctcg tagaacttcg cggcgtccac gtcgcgctgc tcgcgcagcc ccgattcgga
     1141 ctcgagcacg ctgcggatcg cctcgggctc gttgccgtcg accagcaact gcaggccgcg
     1201 gcgcgcgaag gcatcgggtt ccagttccgc gaccgcctcg agcccgagca gcccctcgcg
     1261 ccgcgcggtg gtggcccagc gcaccacctg cttcacgccc tcgccaaagc catggcgcgg
     1321 cggcacgaag acccagcgca tcatggccag cgcgcgcctc agtcccgcga gcggcatctg
     1381 cagcagtccg gcgcccacgg tgccgcccac gaccagcagc gccgccggca ggttcgccag
     1441 cgccgacaac tcgcccccct cgagcaggtt ccccccgacc acggcggtga aggcgagcac
     1501 gatgccgatg atgctcagta cgtccatggt gcctcgctgc tcccgccctg acccacgctc
     1561 accagtcctg cagccgcgcg cgcaggcgct tggtcgcctg gctcaggatc tggctcacgc
     1621 gcgattcgct gacctcgagc acgaggccaa tctccttgag gttcagttcg tcgtcgtagt
     1681 agagcgacag caccagccgc tcgcgctcgg gcaggcgcgc gatctcggcg ccgatcgctc
     1741 ccgccaggcg ctcgcgcgca gcctgctccg cggggttgag cgcggcgctg gcgaaacccg
     1801 agcgctcgcc gtgctcctcg cccatcagtt cgtcgaggct gaacaggcgg ctgccgagcg
     1861 tgtcctgcag catcgcgtgg tactcggcga tctccacccc cagctcgcgc gccaccgcgg
     1921 cgtcgccggc atcgccgccg ctgcgcagct ccacattgcg tatgcccgcg gcgacgcgcc
     1981 gcgcgttgcg gtgcaccgag cgtggcgacc agtcgccgcg gcgcacctcg tcgatcatcg
     2041 cgccacggat gcggatgccc gcgaaggtct cgaagctggc accgcgcgtc gcgtcatagc
     2101 tcgcggcggc ctccagcagc ccgatcatcc ccgactggat caggtcctcg acgctgacgg
     2161 aggacggcag tcgcgccagc aggtggtggg cgatacggcg caccagcggc ccgtgctgct
     2221 cgaccagctc ctgcggctgc gcccgcgcgc ccgtcttcgc gtacatggca agacccgccg
     2281 acaccgccgc tcagcccgcg gcggcatggt cgagcagctg ctcgacgaag aactgcaggt
     2341 ggccgctggg gccggcgggg atgggccagc gctcgacgcg cgcggcaagc tcgcgaaagg
     2401 cggcggccgc gcgcgactgc ggcgcgccca gcagcaccgg gcgctggcgc tggatgcact
     2461 tgcgcaccat gtcgtcgtag ggaatatgcc ccgcatcgag cagcgtgata tcgaggaagc
     2521 ggtcacagac ctgcgccagc ttgtgataga ggctcgcgcc ctcctgcgcg ctcctgacca
     2581 tgttgccgag caggcgaaag tgctgcatgc cgtggtcgcg atgcagcagc ttcatcagcg
     2641 cgtaggcgtc ggcgatcgac gacggttcgt cgcacaccac gaccaggcac tcctgggcgg
     2701 cacgcacgaa actcagcacc gaatccgcga tgccggccgc ggtgtcgatc agcagcacgt
     2761 ccatcccgga accgatcgcg ctgaaggcgt ggatcagccc ggcgtgctcg aggcggctca
     2821 gcgaggccat cgactgcgtg ccggaggatg ccgggatgat gcggattccg ccgggcccgt
     2881 cgagcatgat gtcctcgagg ccgcagccgc cggcgagcac gtcggcgagg ttgcgcttgg
     2941 gccgaaggcc cagcagcacg tcgacgttgg ccagcccgag gtccgcgtcg agcagcacca
     3001 cgcggcgccc gcgcgcggcc agcgcgatag cgaggttgat ggagacgctg gtcttgccca
     3061 caccgccctt gccgccggtg acggcgacga cctggaccgg cctgccggcc ccgtgagcgg
     3121 aattcattcg accttcctcg cgctgggcat gatcaggcta ttctagctgc attgacgccg
     3181 gccgcatcgg gcgctccgtg cccgatgccc gccgcggccg gagttccctc cctggccgct
     3241 tcctccccgg gttcgagggc gagcgcctgc gccaccagct cggccgcgtc cgccacgcgc
     3301 agatcgtccg ggatctccgg tccgtccgtg ctgtaggcca ccggcaggcg ctcgcgccgc
     3361 agcgcgccga tcagctcgcc gaggctcgcg gtctcgtcga gcttggtcac gacggcgccc
     3421 accggcacgc aggccgcgta gtcggccagg gccgcatgga gccacgacag ctgcgcgttg
     3481 gccgcaagca ccagcagcgt gcgcacacct tcgcgcagcg tgcccagctc atcgagttgg
     3541 cggctgcgca cgtgcgcgcg ccggctcacg cccgcggtgt ccacgaggac cagcgcattg
     3601 gccggtacgc ggcgcagcgc ctcgccgatc gattcaccgc tgtcggcggc atggaaaggc
     3661 acgcccagca ggcgcgccac cgcgcgcagc atgtcggcac cgccgaggcg cgcggcatcc
     3721 atgctgagca gcgccagccc cccgggacca tgctccagca cgtggcgcac cgcgagcttg
     3781 cagatggtcg tggtcttgcc ggcgccggtg ggaccgacga aggcccacac cccgcccgag
     3841 gccaccacgt cgcccaccgt gccgagcccc tcgaccagcc gcgcctgcgt ggcgcgccac
     3901 gctgcgtccc aggcggtccc ggcatcgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnc cagccgcgcc tgcgtggcgc gccacgctgc gtcccaggcg gtcccggcat
     4081 cgaccttggc taccagttcg cgcaccagct cgttgggcag gcccatgcgc gtcaagcgcc
     4141 gccagatgcg cccctcggtg cccggcccgt cgacggtgcg ttcgctgcgc agcgctgtca
     4201 ggcgctcttc cagcagcgtg cgcatgctgc gcaactccga ttgcatctcc agccactgcg
     4261 gcgccaaggt gggcccttcg ccggctgtag ggctcgcgac tgcgaacgcc gccggcgcag
     4321 cagttggcgc cgcgccggcg gctgccggta ccgggtcctc cacgcgggcg aatgcgcgct
     4381 ccggcgccga cgggcgcggc cgcggcgcca cgggtgcagc ccccgccgcc aggtcgaacg
     4441 cccgcagcat cggttccccc gcgctcgtgc gcgacgccgg cagcgcgggg gcaggaggcg
     4501 cttccggcgc ggcgacgatc tcgacgcccc cggcgacgcg ccggttcgac aggatcatgg
     4561 cgtcgggacc gagctgctcg cgcacgagcc gcaacgccgt cttcacgtcc gggccgatga
     4621 ttcgcttcag ttccatgtcc tcgctctccc gcagtgacgc ccgcgcgggc gccgatgaga
     4681 attcgtcagc cgacggtcgc ctcgatcgtc acctgcttgt tgccgggaat ttcctggtac
     4741 gagatgaccc gggtcgcgcg attggtgtgg cgcatgaagc gcgccagcac cgcgcgcagc
     4801 ggcgccggca ccagcagcac cggcgccttg ccctgcactt cctggcgctg tgccgcttcg
     4861 ttcatcgcgc gctgcagccg ctctgccagg ctgggctcga gcacgatgtc atcggcctcc
     4921 gcccccgcgg cgccgccgcg ctgcagcgac ttcatcagca cctgttccat ctgcggctcc
     4981 agcgtgatca ccggcaggct cgcggcgttg ccgctgatct gctggaagat ggtgcgaccg
     5041 agggccacgc gcgccgccgc gctcagtgcc gccgggtcct tgctgcgaac ggcctgcgcg
     5101 gcgatcgcct cggcgatgct gcggatgtcg cgtatcggca ctccctcgcc cagcaggctc
     5161 ttcagcacgc cgagcagctg gttgatggtg agcgtgccgg gcaccagttc ctcggcgagc
     5221 ttcggggaca tcctggcgag cttctgcagc agctgctcca cctcttcgtg gccgatcagc
     5281 tcgtcgatgt gctcaagcag cacctggttg aggtgcgtgg cgaccaccgt gctggcatcc
     5341 accacggtgt agccgagggt ctgcgcacgc tcgtgctggt tgcgctcgat ccacagcgca
     5401 tcgagcccga aggccggatc gcggcattcg aggccgtcga gccggccgaa cacctggccc
     5461 ggattgatcg ccagctcgcg ttccgggaac acttccgcct cgcccatcgc cactcccatc
     5521 agcaggatcc ggtaggcatt gggcagcagc tccaggttgt cgcggatgtg caccggcggc
     5581 accaggaagc ccatttcctg cgacagcttc ttgcgcacgc ccttgatgcg acccagcaac
     5641 gcaccgccct gcgccttgtc caccatcggg atcagccggt agccgacctc cagcccgatc
     5701 acgtcgaccg gcgccacgtc gtcccagccc agttcgggcg ccggcgccgg cgccacgcgc
     5761 gcgagctcgg aggccgcggc cggcccgctc gcctcctcgc gccggcgacg gcggatcagc
     5821 cacgccccgg cgcctgcgag cgcggccagc ccgatgaacg ccacgtgcgg catccccggc
     5881 accagcccca tgatcgccag cagcgccgct gccacggcca gcgcgcgcgg cgaatcgaac
     5941 atctgcccga ggatctggtc gcccatgtcg ttcgacgtgg tcac
//
LOCUS       DAORUQ010000183         5954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15869_length_5954_cov_5.828106, whole genome shotgun sequence.
ACCESSION   DAORUQ010000183 DAORUQ010000000
VERSION     DAORUQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15869_length_5954_cov_5.828106"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLW13_13295"
     CDS             <1..186
                     /locus_tag="PLW13_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQY71403.1"
                     /translation="QCGFCTPGVVMTLAALTIDGQEPDEERLLGALSGHVCRCTGYQG
                     MRRAARKLAGAAAGEAP"
     gene            183..2924
                     /locus_tag="PLW13_13300"
     CDS             183..2924
                     /locus_tag="PLW13_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQY71404.1"
                     /translation="MSAPGITTGRYIGQRVRRKEDPRLLTGRGQFVDDIALPGMLHVA
                     FARSPVARGRILSIDTAVARDIPGVHAIYTQADLARFNVNMISFYFSPQDAETTPLAN
                     ARVAYVGEPVALVIADSRHVAEDAASLVEVEYAEEDPVVTIADARRGAPVHPGMTDNV
                     NAQMGDEEIDEELKALLEGAPHLITHKVVHQRISQSPMETRGVVVTRDGTEELTLYIT
                     CQSPHLVARWVSLALGLPQAAIRVIAKDVGGAFGLKNHPWKEEVAVILAALAFGRPLK
                     WIEDRYENLIGANQAREQEMVLQVAFDAEGKLIASHGDYSVNVGAFPQLSECNIAVHM
                     FLWAAYKMPAYGFLTRGWYSNTVGYAAYRGPWAMESLIRETALDVAARRIGIDPIEIR
                     RRNLVTLKDQPATSCMGLPLEDITPAECLEKLLAYFDVAAFRREQEAARGQGRYLGLG
                     IAAYIEPTGAAGSIGTMTGELAQVRIEPTGKVTATLSTHSQGHGTATTMAQIFADRIG
                     VPYEDVAVFEGDSSRGGFSPGAAGSRQGVIAGGAAIMASELLLARVKRLAAHLNGVDP
                     EAIRIEDGMVYVEGEPGKSCPLRAIAEVAYGEPDRLPGGFEAGLEAQYRYQPPPMTFA
                     SAANACVVEVDADTGFVKILRWISSEDCGTPINPAVVEGQIAGGLAQAIGMVLLEQVS
                     FDARGNPKAATYKDYMLPLIADIPDFEFVHANTPSKSVGGMRGVGEGGAIIGPPTLVN
                     AIADALAPFGEVPLELPLTPAKLLGVIEGRDLALCEEPKSGSGTTVVERAAEPSPSVA
                     AAVSAAGAQAAETPESAPAGVDGDWKMVLATPMGPQEMTGHFETRGQSLGGYLRSAEG
                     EQGFSGTVEGNRLKFDLKVEKPMKITLKYDLTVDGDRISGKVKMGIFGSARLSGHRA"
     gene            3016..4203
                     /locus_tag="PLW13_13305"
     CDS             3016..4203
                     /locus_tag="PLW13_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71405.1"
                     /translation="MSSDDSVYYDPYKVEITLDPYQVYRRLREEAPLYYNEQHDFYAV
                     SRYDDVEAGLSDNETFSSARGGILEIIKMNVEMPSGVFIFEDPPLHTVHRGVLSRVFT
                     PKKMNALEPKIREFCARCLDPLVGSGRFDFVADLGAQMPMRVIGMMLGIPEQDLSDVR
                     ERSDASLRTEAGRPMEDFDPSGGDMMQEYIDWRIKHPSDDLMTELLTAEFTDETGTRR
                     RLTREEVIIFCNVLAGAGNETTNRLIGWTGKLLAEHPEQRRQLAADHSLIPNAIEEIL
                     RYEPPGPHIGRYVTRDTEFHGRKVPEGSAILFLVGAANRDHRRFPDGDRFDIRREKRP
                     NLAFGWGIHTCIGAALTRVEGRVALEEVLKRFPEWDVEWDKAKLSPTSTVRGWETLPV
                     VTC"
     gene            complement(4245..4916)
                     /locus_tag="PLW13_13310"
     CDS             complement(4245..4916)
                     /locus_tag="PLW13_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71406.1"
                     /translation="MTRAKTKPSRKARATSSEPATQRRGYNSPVRRQQSAETRERIIA
                     AGAELVHGFPAWDWKNLSAGAVGEHAGISERTVHRYFSTERLLRDAVLQRLVEESGVS
                     LEGLELDDFAGVIARMFSYLSSFAVAPSQVSDPTFASMDRQRREALLNAVIGATPGWP
                     DHDQETAAAVLDILWSLPPYERMITSWGFGAERAIGAITWLIGLIEEAIRNGRRPDLA
                     VSVLR"
     gene            5101..>5954
                     /locus_tag="PLW13_13315"
     CDS             5101..>5954
                     /locus_tag="PLW13_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQY71407.1"
                     /translation="MSEAFIIDAVRTPRGIGKPGKGALSGMHPQHLAATVLKALQERN
                     GLNTADVDDVIWGTSAQKGGQGGDLGRMAALDAGFDIRVSGVTLDRFCGSGLTATNLA
                     AAQIMSGMEDLLIAGGTEMMSLITALGQQEMAAGLRPAGGIGSGNARLQLRHPQSHQG
                     LCADAIASMEGFDRATLDAFALESQRRAAVAIREGRFDRSIVPVIDDEGKVVLASDEH
                     PRPQTTAQELAALKPSFAQIADMAPGADGVTFRQLINRRYPDLKIEHFHHAGNSSGVV
                     DGAAALLLA"
BASE COUNT         1018 a         1929 c         2068 g          939 t
ORIGIN      
        1 caatgcggct tctgcacgcc gggcgtggtg atgacgctcg ctgcactcac catcgacggg
       61 caggagccgg acgaggagcg gttgctcggg gcgctgtccg gccacgtctg ccgctgcacc
      121 ggttaccagg gtatgcgccg cgcggcgagg aagctggctg gcgccgctgc gggagaagcc
      181 ccatgagcgc ccccgggatc accacggggc gctacatcgg ccagcgcgtg cgacgcaagg
      241 aagacccgcg cctgctgacc gggcggggcc agttcgtcga cgacatcgcg ctgcccggca
      301 tgctgcacgt cgccttcgcg cgcagtcccg tggcgcgcgg caggattctc tcgatcgaca
      361 ccgccgtggc gcgcgacatc cccggcgtgc atgcgatcta tacgcaggcg gacctggctc
      421 gcttcaacgt caacatgatc agcttttact tctcgccgca ggacgcggag accacgccgc
      481 tggccaacgc gcgcgtggcc tacgtcggcg agccggtggc gctggtcatt gccgacagcc
      541 gccatgtcgc ggaagatgcg gcctcgctgg tcgaggtcga gtacgcggaa gaggatccgg
      601 tcgtcacgat cgccgacgcg cggcgcggcg cgcccgtgca tccgggcatg acggacaacg
      661 tcaatgcgca gatgggtgac gaagagatcg acgaggaact gaaggcgctg ctcgagggcg
      721 cgccgcacct gatcacccac aaggtcgtgc accagcgcat ctcgcagtcg ccgatggaga
      781 cgcgcggcgt ggtcgtcacg cgcgacggca ccgaggaact gacgctctac atcacctgcc
      841 agagcccgca cctggtggcg cgctgggtgt cgctggcgct cggcctgccg caggcggcta
      901 tccgcgtgat cgccaaggac gtgggcgggg ccttcgggtt gaagaaccac ccgtggaagg
      961 aggaagtcgc ggtgatcctc gccgcgctgg cattcggccg gccgctgaaa tggatcgagg
     1021 accgctacga gaacctgatc ggcgccaacc aggcgcgcga gcaggaaatg gtgctgcagg
     1081 tcgcgttcga tgccgagggc aagctgattg cttcgcacgg ggactacagc gtcaacgtcg
     1141 gcgcctttcc gcagctttcg gaatgcaata tcgcggtcca catgttcctg tgggcggcgt
     1201 acaagatgcc cgcttacggc ttcctgacgc gcggctggta ctcgaacacc gtgggctatg
     1261 ccgcctatcg cggcccttgg gcgatggaat cgctgatccg cgaaaccgcg ctggatgtgg
     1321 cggcgcggcg tatcggcatc gatccgatcg agatccgccg ccgcaatctc gtgacactga
     1381 aggaccagcc cgcgacctcc tgcatgggtc tgccgctgga ggacatcacg cccgccgagt
     1441 gcctggagaa gttgctcgcg tacttcgacg tcgccgcgtt tcgccgcgag caggaggccg
     1501 cgcgcgggca gggtcgctac ctcgggctcg gcattgccgc ctacatcgaa ccgacggggg
     1561 ccgcgggcag catcggcacg atgaccggcg agctcgcgca ggtgcgcatc gagccgaccg
     1621 gcaaggtcac cgcgacgttg agcacgcact cgcagggtca tggcacggca accacgatgg
     1681 cgcagatctt cgccgaccgt atcggcgtgc cctacgagga cgtcgccgtg ttcgagggcg
     1741 acagttcgcg tggcggtttc tctcccggcg cggcgggcag tcgccagggc gtgatcgcgg
     1801 gcggtgcggc gatcatggcc tcggaactgc tgctcgccag ggtgaagcgg ctggccgctc
     1861 acctgaacgg tgtcgatccc gaggcgatcc gcatcgagga cggcatggtc tacgtcgagg
     1921 gtgaacccgg gaagtcctgc ccgctgcgcg cgatcgcgga ggtggcctac ggcgagcccg
     1981 atcgcctgcc gggcgggttc gaggccgggc tggaggcaca gtaccgttac cagccgccgc
     2041 cgatgacctt cgccagtgcc gccaatgcct gcgtggtcga ggtggacgcc gataccggct
     2101 tcgtcaagat cctgcgctgg atatcctcgg aagactgcgg cacgccgatc aaccccgcgg
     2161 tggtggaggg ccagatcgcc ggtggcctgg cccaggccat cggcatggtg ctgctggagc
     2221 aggtgtcctt cgacgcgcgc ggcaacccga aggcggcgac ctacaaggac tacatgctgc
     2281 ccctgatcgc cgatatcccg gacttcgagt tcgtccacgc caatacgccg tcgaaatcgg
     2341 tgggtggcat gcgcggtgtg ggcgagggcg gtgcgatcat cggcccgccg acgctggtca
     2401 acgccatcgc cgacgcgctg gcgcccttcg gcgaggtgcc cctggagttg ccgctgacgc
     2461 cggcgaagct gctcggcgtc atcgaggggc gcgatctggc gctttgtgaa gagccgaagt
     2521 ccggcagtgg cacgaccgtt gtcgagcgtg ccgccgagcc gtcgccgtcc gtcgccgccg
     2581 cggtatcggc ggccggcgcg caggcggccg agactccgga gtccgcgccg gcgggcgtag
     2641 acggggactg gaaaatggtg ctcgccaccc cgatgggccc gcaggaaatg acgggccatt
     2701 tcgaaacccg gggccagtcg ctcggcggtt acctgaggag cgcggaaggc gagcagggct
     2761 tcagcggcac cgtggagggc aatcgcctga agttcgatct caaggtcgag aagccgatga
     2821 agatcacgct gaaatacgac ctgacggtgg acggtgaccg catcagcggc aaggtcaaga
     2881 tgggcatctt cggatcggcc aggctgagcg ggcaccgggc gtgacagccg gcgtgagctg
     2941 attcgccgcc ctctccttga ccggcatcag aattggtgtc atatagtgga caccaattat
     3001 gcgcaggggc atctcatgag cagcgacgac agcgtctact acgatccgta caaggtagag
     3061 atcacgctcg atccgtacca ggtctatcgg cgcctgcgcg aggaggcgcc gctctattac
     3121 aacgagcagc acgacttcta cgccgtcagc cgctacgacg acgtggaagc gggcttgtcc
     3181 gacaacgaga ccttcagctc ggcgcgtggc ggcatcctcg agatcatcaa gatgaacgtc
     3241 gagatgccat cgggcgtgtt catcttcgag gatccgccgc tgcacacggt tcaccgcggc
     3301 gtgttgtcgc gggtattcac gcccaagaag atgaacgcgc tcgagccgaa gatccgcgag
     3361 ttctgcgcgc gctgcctcga tccgctggtc ggcagcggca ggttcgactt cgtcgccgac
     3421 ctcggcgccc agatgccgat gcgcgtcatc ggcatgatgc tcggcatccc ggaacaggat
     3481 ctgagcgatg tgcgcgaacg ctccgatgcg agcctgcgca ccgaggccgg ccggcccatg
     3541 gaggatttcg atcccagcgg cggcgacatg atgcaggagt acatcgactg gcgcatcaag
     3601 catccctcgg acgacctgat gaccgagttg ctgaccgccg agttcacgga tgaaacgggc
     3661 acccggcggc ggctcacccg cgaggaagtg atcatcttct gcaacgtgct cgccggtgct
     3721 ggcaacgaga ccaccaaccg gctgatcggc tggaccggca agctgctcgc cgagcatccc
     3781 gagcaacgcc gccagctggc cgcggaccac tcgctgatcc ccaacgcgat cgaggagatc
     3841 ctgcgctacg agccgccggg gccgcacatc gggcgatacg tcacgcgcga caccgaattc
     3901 cacggccgga aagtgcccga gggcagcgcc atactgttcc tggtcggggc ggccaatcgc
     3961 gaccaccggc gcttccccga cggcgatcgc ttcgatatcc gccgggagaa acgtcccaac
     4021 ctcgcgttcg ggtggggtat ccacacctgc atcggcgcgg cgctgacccg tgtcgaagga
     4081 cgcgtggcgc tggaggaggt cctcaagcgc tttcccgagt gggacgtgga atgggacaag
     4141 gcgaagctgt cgccgacctc cacggtgcgc ggctgggaga cgctgcccgt ggtgacgtgc
     4201 tgagagcgga tcccgggaga agcgtggcgt gagcgcgggc gcagtcagcg cagcacgctg
     4261 actgcgagat cggggcgccg accgttgcga atggcttcct cgatcaggcc gatcagccag
     4321 gtgatggcgc cgatggcacg ctcggcaccg aagccccagc tggtgatcat gcgctcgtag
     4381 ggtggaaggc tccacagtat gtccagcacg gccgccgcgg tctcctggtc gtgatccggc
     4441 cacccgggcg tcgcgccgat caccgcgttc agcagcgcct cgcgccgttg ccgatccatc
     4501 gatgcaaagg tgggatcgct cacctgcgac ggggcgacgg caaacgatga caggtaactg
     4561 aacatgcggg cgatgacgcc cgcgaaatcg tccagctcca atccctccag cgagacgccg
     4621 gactcctcga ccaggcgctg caacacggcg tcgcgcagca ggcgctcggt cgaaaagtag
     4681 cggtggaccg tccgctcact gatgcccgcg tgctcgccga ccgcgccggc gctgaggttc
     4741 ttccagtccc acgcgggaaa accatgcacg agctcggcgc cggcggcgat gatgcgctcg
     4801 cgggtctcgg cgctctgctg tcggcgcacg gggctgttgt agccgcgccg ctgcgtcgcc
     4861 ggttcgctcg aggtcgcgcg ggctttacgt gacggcttgg tcttggctct tgtcatgtgc
     4921 atcgctgccg ggatggctgg tacggatgtg cggatgctac cagtgtatgc gccggccggt
     4981 cattcgcacg catcgacgca ctagacaaca tcgtcgacca atagccgggg aacgtgtata
     5041 taatgaaaaa tgttttccgg tcggttgtgc gcggccgcag tctagcagag ggagagtgat
     5101 atgtcggaag cgttcatcat cgacgccgtg cgcacgcccc gcggtatcgg caagccgggc
     5161 aagggcgcgc tgtcgggcat gcatccgcag cacctcgcgg caaccgttct caaagcgctg
     5221 caggagcgca atggcttgaa taccgctgac gtcgatgacg tcatctgggg aaccagcgcg
     5281 cagaagggcg ggcagggcgg ggacctcggg cgcatggccg cgctggatgc gggattcgat
     5341 atccgcgtga gcggcgtgac gctggaccgc ttctgcggca gtggtcttac ggccaccaat
     5401 ctcgccgcgg cgcagatcat gtccggtatg gaagacctgc tcatcgccgg cggcaccgaa
     5461 atgatgtcgc tcatcacggc gctgggacag caggaaatgg cggccggctt gcgcccggcg
     5521 ggaggaatcg gttcgggcaa cgccaggctg cagttgcgcc atccgcaatc gcaccagggc
     5581 ctgtgtgccg acgcgatcgc atcgatggaa ggtttcgatc gcgcaacgct ggatgccttt
     5641 gccctcgaga gccagcgacg cgccgccgtc gcgatccgcg aagggcgatt cgaccgatcg
     5701 atagtccccg tgatcgatga cgagggcaag gtcgtgctcg cgagcgacga gcacccgcgc
     5761 ccgcagacca cggcgcagga gctcgcggcg ctgaagccga gcttcgccca gatcgccgac
     5821 atggcgccgg gcgctgacgg ggtcaccttc cgccagctca tcaaccggcg ctatccggat
     5881 ctgaagatcg agcacttcca tcatgcgggc aattcctccg gtgtggtcga cggtgccgcg
     5941 gcgctgctgc tggc
//
LOCUS       DAORUQ010000184         5934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_15959_length_5934_cov_5.637353, whole genome shotgun sequence.
ACCESSION   DAORUQ010000184 DAORUQ010000000
VERSION     DAORUQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15959_length_5934_cov_5.637353"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLW13_13320"
     CDS             <1..286
                     /locus_tag="PLW13_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HQY71408.1"
                     /translation="GAPGAYDYGPERCSWLTHHVTNWMGDDGFLRKSKCQIRRHNPDG
                     DAIFIDGTVSRKFVEGDRHLVEISQKAETHRGETSAFGTSIVELPSRTGK"
     gene            362..1576
                     /locus_tag="PLW13_13325"
     CDS             362..1576
                     /locus_tag="PLW13_13325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY71409.1"
                     /translation="MHESGSSSWAGPLRGIRVLDFTRVLSGPAAALALADLGAEVLKI
                     EPPGAGDETRTFPPMRAQESHYFLSVNRGKKSIVIDLKTEAGVKLARDLARECDILIE
                     NYRPGVMDRLGLGYDTLSALNPRLIYCAISGFGMTGPLRDRPSFDIVMQALSGALSVN
                     GEPGREPTKLGIPLGDLVGGVNGPIAILAALYERSLTGRGRLIDISLLDGLIGMLGYL
                     PQLAWFTGQDPAPQGSQHPNLVPYGVFPASDGSIVIACLTNSFWANICRSLGMDDWIA
                     DPRFDTIEKRRDTRALVNERICAITSGRTVNELVEIFVAHQVPHAPILGIREALSQPQ
                     AVAREMVVETEHRSLGRIPIVNRSIRFPGDPQPVPTAPPVLGQHTDEILGDILGLSAE
                     QIGELRASGVVA"
     gene            1648..2580
                     /locus_tag="PLW13_13330"
     CDS             1648..2580
                     /locus_tag="PLW13_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71410.1"
                     /translation="MTQLRFEDRVAVITGAGRGLGRAYALLLAARGAKVVVNDPGASL
                     QGESTDAGPAQAVVREIEAAGGEAVACTESVATPAGGKAIIQSALDNYGRIDILIHNA
                     GIVRRGSLQELSYEDFELVLDVHLRGGFHVVRPAFPLMCRAGYGRIVLTGSINGLYGN
                     RNVANYSVAKAGLIGLSNVAALEGAEHGVKSNIILPAAVTRMAEGLDTSAYPPMDPEL
                     VAPAVGWLAHESCSVTGEMLIAMAGRVARAYAAETPGVYHPAWSIEQVGEEIDAIRNT
                     DAPVVFPVVPSGHVDHLRYSFGMAAAGDAREPGK"
     gene            2605..3057
                     /locus_tag="PLW13_13335"
     CDS             2605..3057
                     /locus_tag="PLW13_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQY71411.1"
                     /translation="MAGRYFDDWQVGNRIEHEIRRTVTETDNLLFSVMTHNPQPLHID
                     VEAAKASEFGQILVNGTFTFALMIGLTISDTTLGTLVANLGYDKLVMPKPVFIGDTMR
                     ATTEIAELRESKSRPNAGIVVFAHELINQRDEVVCRCLRTALINRRPV"
     gene            3330..3818
                     /locus_tag="PLW13_13340"
     CDS             3330..3818
                     /locus_tag="PLW13_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQY71412.1"
                     /translation="MKDAGSNIDTVRVLAREFRALPGALLPLLHAIQDELGYVPEPAI
                     PVIAQELNLSRAEVHGVISFYHHFRREPPGRHIVQLCRAESCQALGARTLEAEVKALL
                     GIDFHQTTPDRCITLEPVYCLGNCACSPAIRIDDEIHGMVDARELAALLEDLRTQIIE
                     VR"
     gene            3820..5385
                     /locus_tag="PLW13_13345"
     CDS             3820..5385
                     /locus_tag="PLW13_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQY71413.1"
                     /translation="MSRITVYVPCDTSAVSLGADRVAERLARECRDQPVDIVRNGSRG
                     AFWLEPLLEVEAGGQRIAYGPVRPDDIPRLLEEGMLSASTRSALCLGVVDEIPFLKQQ
                     QRLTFARQGVVDPVSVASYRAHGGFAGLERALGMSPRDIVEEVTESGLRGRGGAAFPT
                     GIKWQTVLNAPGERKYIVCNADEGDSGTFADRMLMEGDPMSLIEGMIIAGLAVGATRG
                     YIYLREEYPRAGRMLQQAIAAVREAGYLGERIYGSAHGFDMELRIGAGAYICGEETSL
                     LESLEGKRGVVRAKPPLPAIAGLFGQPTVVNNVISLATVPVILARGAPFYRDFGMGRS
                     RGTLAFQLAGNIARAGLVELAFGSTLRELLFDFGGGTRSGRPLRAVQVGGPLGAYLPE
                     AHWDTPMDYEAFAAVGAMVGHGGIVAFDDTVNMREQARFAMAFCAEESCGKCTPCRIG
                     STRGVEVIDRIAAGSDVDANLVLLQELCEAMTDGSLCALGGMTPYPVLSALGHFAEDF
                     GVRGESSGSRESA"
     gene            5385..>5934
                     /locus_tag="PLW13_13350"
     CDS             5385..>5934
                     /locus_tag="PLW13_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQY71414.1"
                     /translation="MLWIYDPATQGAARDYGTPAPVPEYSAEYAALEQVSLSIDGRQV
                     SVPRGTSVLRAAALAGINIPKLCATDALDAFGSCRLCLVEIAGRKGYPASCTTPVEEG
                     VAVTTQNGKLARLRRNVMELYISDHPLDCLTCPANGNCELQDMAGAVGLREVRYGYDG
                     RNHLDAKKDTSNPYFTFDPSKCI"
BASE COUNT          970 a         1943 c         2077 g          944 t
ORIGIN      
        1 gggcgcgccg ggcgcctacg actacggtcc ggagcgttgc tcgtggctga cgcaccacgt
       61 caccaactgg atgggcgacg acggcttcct gcgcaagagc aagtgccaga tccgccgcca
      121 caatcccgat ggcgacgcga tcttcatcga cggcaccgtc tcccgcaagt tcgtcgaggg
      181 cgacaggcac ctggtggaaa tcagccagaa ggccgagacg caccgtggcg aaacctccgc
      241 cttcggtacg tccatcgtcg aactgccgag ccgcaccggc aagtaagcgg ctgtccgtcc
      301 cggccgcact ccctggcggg agtgcggccg acggcacgca actcatcgca aggcggcgcg
      361 tatgcatgag agtggcagtt cgagctgggc cggcccgctg cggggaatcc gcgtgctcga
      421 cttcacccgc gtgctgtcgg ggccggcggc ggcgctggcg ctggccgatc tcggtgccga
      481 ggtgctgaag atcgagccgc cgggcgcggg cgacgaaacc cgtaccttcc cgccgatgcg
      541 cgcgcaggag agccactatt tcctgagcgt gaaccgcggc aagaagagca tcgtcatcga
      601 cctgaagacc gaggccggcg tgaagctggc ccgggatctc gcgcgcgagt gcgacatcct
      661 gatcgagaac taccgccccg gagtcatgga tcgcctcggg ctcggctacg acacgctgtc
      721 ggcgctcaat ccgcggctga tctattgcgc gatctcgggc ttcggcatga ccggcccgct
      781 gcgcgaccgg ccgtccttcg atatcgtgat gcaggcgttg tcgggcgcgc tcagcgtcaa
      841 cggcgagccc gggcgcgaac ccaccaagct ggggattccc ctcggcgacc tggtgggcgg
      901 ggtgaacggt ccgatcgcca tcctcgcggc gctgtacgaa cgcagcctca cgggccgcgg
      961 acgcctgatc gacatcagcc tgctcgatgg cctgatcggc atgctcggct acctgccgca
     1021 actggcgtgg tttaccgggc aggatcccgc gccgcaggga tcgcagcatc ccaacctggt
     1081 gccctacggg gtatttccgg cgagcgacgg ctcgatcgtc attgcctgcc tgaccaacag
     1141 tttctgggcg aacatctgcc gctcgctcgg catggacgac tggatcgccg acccgcgttt
     1201 cgacacgatc gagaagcgcc gagacacgcg cgcgctggtg aacgagcgga tctgcgcgat
     1261 caccagcggc aggaccgtga acgaactcgt ggagatcttc gtcgcccacc aggtcccgca
     1321 tgcgccgatc ctgggcatcc gcgaggcgct gtcgcagccg caggcggttg cccgggaaat
     1381 ggtggtcgag accgagcaca ggagcctggg aaggatcccc atcgtgaacc gatcgatccg
     1441 ctttcccggc gatccgcagc cggtcccgac cgcgccaccg gtgctcgggc agcacaccga
     1501 cgagatcctg ggggatatcc tcgggctctc ggccgagcag atcggggagc tgcgcgcgtc
     1561 cggggtcgtg gcctgagccg gccgcccgat cccggcgaga gcgctgcgac tggcctcgtc
     1621 cgacaccgaa cagcagagga gagtccgatg acacagctca gattcgaaga tcgcgtggcg
     1681 gtcatcaccg gcgcgggacg aggcctgggc cgtgcctatg cgctgctgct cgccgcgcgg
     1741 ggcgcgaaag tcgtcgtcaa cgatccgggt gccagcctgc agggcgaaag caccgatgcc
     1801 ggccccgcgc aagcggtggt ccgggagatc gaggcggcgg gcggtgaggc ggtggcctgc
     1861 accgaatcag tggcaacgcc cgcgggcggc aaggccatca tccagtcggc gctcgacaac
     1921 tacgggcgca tcgacatcct gatccacaac gccggcatcg tgcgccgcgg atccctgcag
     1981 gagctgtcgt acgaggattt cgagctggtc ctcgacgtgc atctgcgcgg cggcttccat
     2041 gtcgtgcgcc ctgcatttcc cctgatgtgc agggccggct atggtcgcat cgtgctgaca
     2101 ggctcgatca acggcctcta tggcaatcgc aacgtggcga actacagcgt ggcgaaggcg
     2161 ggcttgatcg gcctgtccaa cgttgctgcg ctggaaggcg ccgaacacgg cgtgaagagc
     2221 aatatcatcc tgcctgccgc ggtcacgcgc atggccgagg ggctcgatac cagcgcctac
     2281 ccgccgatgg accccgagct cgtggccccc gcggtgggct ggctcgccca cgaatcctgc
     2341 tcggtcacgg gcgagatgct gatcgccatg gcgggccgtg tcgcgcgggc ctatgctgcc
     2401 gagacgcccg gtgtctacca tccggcgtgg tcgatcgagc aggtgggcga ggagatcgat
     2461 gcgatccgca acaccgatgc gccggtggtg ttcccggtcg tgccctcggg gcatgtcgac
     2521 catcttcgct acagtttcgg gatggccgcg gccggcgatg cgcgggagcc gggcaagtaa
     2581 tacggatacg gaaggaggac ggggatggcg gggcgctatt tcgacgattg gcaggtcggg
     2641 aatcgcatcg agcacgagat ccgccgcacc gtgaccgaga ccgacaacct gctgttctcg
     2701 gtcatgaccc acaacccgca gccgctgcat atcgacgtcg aggcggccaa ggcctccgag
     2761 ttcgggcaga tcctcgtgaa cggcaccttt accttcgcgc tgatgatcgg cctgaccatc
     2821 agcgacacca cgctcggcac tctcgtcgcc aacctcggct acgacaagct ggtgatgccc
     2881 aagcccgtgt tcatcggcga caccatgcgc gccaccaccg agatcgcgga gctgcgcgag
     2941 tcgaagtcgc ggccaaacgc cggcatcgtc gtctttgccc acgaactgat caaccagcgc
     3001 gacgaggtcg tctgccgctg cctgcgcacc gcgctgatca accgcaggcc ggtgtaggat
     3061 ccatcgggat cgataccggg ggcgcaggca ttgcagcgct gccagttccg tttgtgctgc
     3121 ctgtggcaga aatcggacag gaatatctgc tgcccggtgt gaaggcgcgg ggtggagctc
     3181 gttgtctgaa tggagtacgt ttgcctccgg gcgcagccct cggcgaggcg aatcaccaaa
     3241 gaagaacgtg gcgcgcgcgt cggggttccc cggggctgtg gggcgatggc gcgggcaaag
     3301 gacatttttc ccgggacaag gatcgattgg tgaaagacgc cggctcgaac atcgacaccg
     3361 tgcgcgtcct ggcgagggaa ttccgtgcgc tgcccggagc cttgctgccg ttgctgcatg
     3421 ccatccagga tgagctgggg tacgtgccgg agccagccat tcccgtgata gcgcaggagc
     3481 tgaacctctc gcgcgcggaa gtgcacggcg tcatcagttt ctatcatcat ttccgccgcg
     3541 agccgccggg gcgccacatc gtgcagctct gtcgtgccga gtcctgccag gcgctcgggg
     3601 cacgcacgct ggaagccgag gtaaaggcgc tgctgggcat cgatttccac cagaccacgc
     3661 cggatcgctg catcacgctg gagcccgtgt actgcctcgg caattgcgcc tgttcgccgg
     3721 ccatccgcat cgacgacgag attcacggca tggtggatgc gcgcgagctg gcggcgctgc
     3781 tggaagatct gcgcacccag atcatcgagg tgcgctgaga tgtcgcgaat cacggtttat
     3841 gtgccctgcg acaccagcgc cgtttccctc ggcgccgacc gggtggccga gaggctggca
     3901 cgggaatgtc gcgaccagcc ggtggacatc gtgcgcaacg gctcgcgcgg ggccttctgg
     3961 ctggagcccc tgctggaggt cgaggccgga ggtcagcgca tcgcctacgg gccggtacgg
     4021 ccggacgaca tcccgcgcct gctggaggag ggaatgctga gcgccagcac acgctcagcg
     4081 ctgtgcctgg gcgtggtgga cgagattccg ttcctgaagc agcagcagcg actgaccttc
     4141 gcgcgccagg gcgtggtcga cccggtgtcg gtggcgagct atcgcgcgca cggcggcttc
     4201 gcggggctcg agcgcgcgct cggcatgtcg ccgcgcgata tcgtcgaaga ggtcaccgaa
     4261 tcggggctgc gcggacgcgg tggcgccgcg ttcccgaccg gcatcaagtg gcagaccgtg
     4321 ctgaacgcgc ccggggagcg gaagtacata gtctgcaatg ccgacgaggg cgattccggc
     4381 acgtttgcgg atcgcatgct gatggaaggc gacccgatga gcctgatcga gggcatgatc
     4441 atcgccggcc tggcggtggg cgcgacccgc ggctacatct atctgcgcga ggagtacccg
     4501 cgggccgggc ggatgctgca gcaggccatc gccgccgtgc gtgaagcggg gtacctgggc
     4561 gagcgcatct acggcagcgc ccacggcttc gatatggaac tgcgcatcgg cgccggcgcg
     4621 tacatctgcg gcgaggagac atcgctgctg gaaagcctgg agggcaagcg cggggtggtc
     4681 cgggccaagc cgccgttgcc ggcgatcgcc gggctgttcg gccagccgac agtggtcaac
     4741 aacgtgatct cgctcgccac ggtgccggtg attctcgccc gcggcgcgcc gttctatcgt
     4801 gatttcggca tgggacgctc gcgcggcacg ctggcgttcc agctcgcggg caacatcgca
     4861 cgcgccgggc tggtggagct cgccttcggc agcacgctgc gcgagttgct gttcgatttc
     4921 ggcggcggca cgcgcagcgg gcggccgctg cgcgcggtgc aggtgggcgg accgctcggc
     4981 gcctatctgc ccgaggcaca ctgggatacg cccatggact acgaggcttt cgcggccgtc
     5041 ggggccatgg tggggcacgg cggcatcgtg gctttcgatg acaccgtgaa catgcgcgag
     5101 caggcgcgtt tcgccatggc cttctgcgcc gaggagtcct gcggcaagtg cacgccctgc
     5161 cgcatcggca gcacccgtgg cgtggaggtg atcgaccgga ttgccgcggg cagcgatgtc
     5221 gacgccaacc tcgttctgct gcaggagctc tgcgaggcca tgaccgacgg ctcgctgtgt
     5281 gcgctgggcg gcatgacgcc ctacccggtg ctgagcgcgc tggggcattt cgcggaggat
     5341 ttcggcgttc ggggggaatc gtccggttcc cgggagagtg catgatgctg tggatctacg
     5401 accccgcgac ccagggcgcc gcccgggatt acggcacccc ggcgcccgta ccggaataca
     5461 gcgcggaata tgccgcactg gagcaggtga gcctgagcat cgacggccgg caggtgtcgg
     5521 tgcccagggg aacctcggtg ctgcgcgcgg ccgcgctcgc gggcatcaac atcccgaagc
     5581 tctgtgccac cgatgcactc gatgccttcg gctcctgccg cctgtgcctg gtggaaatcg
     5641 ccgggcgcaa gggctacccg gcctcctgca ccacgccggt ggaggagggc gtggcggtca
     5701 cgacgcagaa cggcaagctg gcgcggctgc ggcgcaacgt gatggagctc tacatctcgg
     5761 atcacccgct cgactgcctg acctgcccgg ccaacggcaa ctgcgagctg caggacatgg
     5821 ccggcgcggt cggcctgcgc gaggtgcgct acggttacga cggcaggaat cacctcgacg
     5881 cgaaaaagga cacctcgaac ccctatttca ccttcgaccc gagcaagtgc atcg
//
LOCUS       DAORUQ010000185         5900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_16114_length_5900_cov_5.604448, whole genome shotgun sequence.
ACCESSION   DAORUQ010000185 DAORUQ010000000
VERSION     DAORUQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16114_length_5900_cov_5.604448"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLW13_13355"
     CDS             complement(<1..760)
                     /locus_tag="PLW13_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71415.1"
                     /translation="MAIERGDVTGLVIPAHAEALRAAGAVFLSAAFQAFGSLDPGTRV
                     ARITRLENCPGGSTGQKLFLSVDYDPPAPHLHTDLFVKFSRDFNDPLRDRGRFEMASE
                     VRFAALSRLADFPVSVPRAYYADYHQDSGTGVLISQRIPFGVDGIEPQHQKCLDHELA
                     DPASYYRAIVRALARLAGAHKGGRLTADVDVQFPFDAQAAAAADRIRYDERQLRELLA
                     AYAEFATKYPQLLPANVRAPGFLARLEHDACRFLA"
     gene            complement(786..1604)
                     /locus_tag="PLW13_13360"
     CDS             complement(786..1604)
                     /locus_tag="PLW13_13360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71416.1"
                     /translation="MPSVADVSSRSITELNSLAGRSAVVTGGGRGLGKAIARRLAEAG
                     ANVLVGDIDAALARSAAEELDASGSARVLGAVMDVTDSATVKVAADRAVREFGGLDIW
                     VNNAGIFPCVPLPEMSDELWDRVLAVNTRGVFAGAREAARCMRAGARGGVIVNVASTA
                     GFKGVAPGLAAYVSSKHAVRGMTRQLALELAPDAIRVLGVAPTFCVTEGNLLAMSEHT
                     DVAITAEQIPAMLSSRLGRVGVPDDVARAVLFCASDMSVFMTGSTLLVDAGETI"
     assembly_gap    1906..2005
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2138..3373)
                     /locus_tag="PLW13_13365"
     CDS             complement(2138..3373)
                     /locus_tag="PLW13_13365"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71417.1"
                     /translation="MDIEQPDWVRRLNYLGAMVGGSRRLVSLAPEELIEEARASTGLA
                     DFGEPGWEDAFRALVESLQTEVDLHTIGRLVTRAELLRGLRNRLLVTEACRAQPAIRH
                     EQVVAPLLIAGQGRTGTSILFELLALDTNSRAPLAWEAASPAAPPGATLAEGITREQI
                     AETVNEFLSDVQPAIRAAHEHRWDLPVECIRFMDSEFSSDWWAMLYAAWGWLKWRAEH
                     PTDAPYRWHRRVLQLLQYGHGDARRWLLKSPAHLGNLDRLLRQYPDIRIIHTHRDPVR
                     SVPSTVSLSTLMRTSRANGVDANLLGQMVVMGYGGMLRKVIEERGGGVIPPGQITDVH
                     FKDLMNDPVGTVERTYDALGLPFTAAFAEAIGDYLAKKPRDRFGKHRYAAEDYGLSNA
                     GIREAFRFYTDHYGIALED"
     gene            3540..4643
                     /locus_tag="PLW13_13370"
     CDS             3540..4643
                     /locus_tag="PLW13_13370"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQY71418.1"
                     /translation="MTESRRAFRELLELLGQIDAQYVGETGEYKSALDVADGHRFILH
                     GLRRALGSQLEADTRRPVFQRAVTPTTKFGGDSPDAIYHECNVSADCSYRIRGDMAGA
                     VYVSLSVQSGGGEAEGVGASINSEQFEVRPDGSFEILLSRAPPADSRNWVRMPEGALN
                     VLTRHYFEEAGNVMADTARRLDLEIDALGDFPAPREPTVESITAGIERVSRWMRSRTL
                     EESKGRTTMPAWVSVVPNRFNEPEKPGADMAYAALDIAYAMAPFHVPPGKALVISGRF
                     PACRYAGVVLWSRFRQSFDYMNRRVSLNRAQTVTDGAGNYRIVLAERDPGVPNWLDTG
                     GRASGFMYWRFLLPEEPITAPQCELVDIDSLLR"
     gene            complement(4657..>5900)
                     /locus_tag="PLW13_13375"
     CDS             complement(4657..>5900)
                     /locus_tag="PLW13_13375"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY71419.1"
                     /translation="MYAHEAPAMSNPPNTDHPRTDDVPVAGFCDPAFAEVEHAFRENF
                     RTRNELGAAACVRIGGRVVIDLWGGHRDAGRTETWQRDTLVNIYSVGKGIASMLVLGL
                     VERGELALDRPLREIWPEIAAAGKERLTLRMLLAHRGGLPGVRRPLPSDAMYDWSTMT
                     VELSAQAPYWEPDAAHGYHVNTHGFLIGEPVVRRLGMPFAAALRERLTGPLEADYHVG
                     LPLREHARVAAIVEADAAPITDGQSAVRAHLASGDPATDAMLASVYFNPPGISGFGIV
                     NRPQWRSAAIPSTNAHATARAVALLYDVFMHRDAAAGGIVGPGLRAQACSIQSDGPDR
                     VLGKHSRFGLGFQLSRPGRRIGGSERGYGHFGYGGSLGFADPASGVAFAYVTNRPGKR
                     FESARTDALLEPLYHALGASG"
BASE COUNT          938 a         2053 c         1946 g          863 t
ORIGIN      
        1 gcgcgaggaa gcggcaggcg tcgtgttcca gcctcgccag gaaaccgggc gcgcgaacat
       61 tggccggcag gagctgtgga tacttcgtcg cgaactccgc gtacgcggcg agcagctcgc
      121 gcaactggcg ctcgtcgtag cgaatccggt cggccgcggc cgccgcctgc gcatcgaagg
      181 gaaactgcac atcgacatcg gccgtcaggc ggccgccctt gtgcgcgccg gccaggcggg
      241 ccagcgcccg gacaatcgct cggtaatagc tcgccgggtc ggccagctcg tggtcgagac
      301 acttctggtg ctgcggctct atgccgtcca cgccgaaggg aatgcgctgc gagatcagca
      361 cgccggtgcc ggagtcctga tggtagtcgg cgtagtaagc cctgggcacg ctgaccggaa
      421 agtccgccag tcgcgacagg gcggcgaagc gcacctccga ggccatctcg aacctgccac
      481 ggtcgcgcag cggatcgttg aaatcacgcg agaacttgac gaagagatcg gtatgcagat
      541 gcggcgcggg cggatcgtaa tcgaccgaca ggaacagctt ctggcccgtg ctgccgcccg
      601 ggcaattctc caggcgagtg atccgcgcca cgcgcgtacc gggatcgagc gagccgaagg
      661 cctggaacgc cgcgctcaga aagactgcac ccgcggcgcg cagcgcctcg gcgtgcgccg
      721 ggatcaccag gcccgtgaca tcgccgcgct cgatcgccac tgaatcctcc acggctggca
      781 gcaaatcaga tcgtctctcc cgcatccacg agcagcgtgc tgcccgtcat gaagacagac
      841 atgtccgagg cgcagaacag gaccgcacgc gccacatcgt cgggaacccc cacgcgcccc
      901 aggcggctac tcagcatcgc ggggatctgc tctgccgtga tcgcgacgtc ggtgtgctcc
      961 gacatggcca gcagattgcc ctcggtcacg cagaaggtgg gcgccacgcc gagtacgcgg
     1021 atcgcatcgg gcgccagctc cagcgccagc tggcgggtca ttccgcgcac ggcgtgcttg
     1081 gacgacacgt aggccgcgag cccgggcgcc acgcccttga aaccggcggt ggacgccacg
     1141 ttcacgatga ctccaccccg cgcgccggcc cgcatgcagc gagcggcctc gcgtgcgccg
     1201 gcgaacacgc cccgggtatt gacggccagc acccggtccc acagttcgtc gctcatttcc
     1261 ggcagcggga cacagggaaa aatgcccgcg ttgttcaccc atatgtccag gccgccgaac
     1321 tcccggaccg cgcgatccgc cgcgaccttc accgtcgccg aatcggtgac atccatgacc
     1381 gcgccgagca cccgcgctga gccgctcgca tccagctcct ccgccgccga gcgtgccagc
     1441 gctgcgtcga tgtctccgac cagcacattg gcaccggcct ccgccaggcg ccgcgcgatg
     1501 gccttgccca gaccgcgccc gccccccgtg acaaccgcgc tgcggccggc gagggagttg
     1561 agttcggtga tggatcggct cgaaacatcg gctactgatg gcatgtgaac gctctccgtg
     1621 agtatcggga tccgtgattg agccgaccgg gcgtggaacg cggaatcgcc agcgcgctgt
     1681 cagttccggt cccttgccgt ggtggcagtg gtgccactcc tgcagcgcgc catggctgct
     1741 atcataccgc acagcacctg aatttttaat gccctcgact cataagcact tatgtcgtgg
     1801 aaaatggcat tattcgggtc atatgctgtt cattagctca gcatatctga ggctcaggcg
     1861 cgacagcggc cgtggccgcc gcaccatgcc cgcagtcact gatagnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnggctc aggcgcgaca gcggccgtgg ccgccgcacc
     2041 atgcccgcag tcactgatag ctgctgccat tccgcgcagg ccggcattca tgcgcacagg
     2101 cgcccttgcc gggctgaagc ccgagctgca gggaggttca gtcctccagc gcgataccgt
     2161 agtgatcggt gtagaagcgg aacgcctcgc ggatgcccgc gttgctgagt ccgtaatcct
     2221 ctgcggcgta gcggtgcttg ccgaaccggt cgcgcggctt cttcgcgagg tagtcgccaa
     2281 tcgcctcggc gaaggcggcc gtgaacggca gccccagcgc atcgtaggtg cgctccaccg
     2341 tgcccacggg gtcgttcatg aggtccttga agtgcacgtc cgtgatctgt cccggcggga
     2401 tcacgccgcc gccgcgctcc tcgatcacct tgcgcagcat cccgccgtag cccatcacca
     2461 ccatctgacc gagcaggttc gcatccacgc cgttggcgcg actggtgcgc atcagcgtgg
     2521 acaggctcac ggtggacgga accgagcgca ccgggtcgcg gtgggtgtgg atgatgcgga
     2581 tgtccgggta ctggcgcagc agccggtcga ggttgcccag atgcgccggc gatttcagca
     2641 accagcgccg cgcgtcgcca tgcccgtatt gcagcaactg cagcactcga cgatgccagc
     2701 gatacggcgc gtccgtcggg tgctcggcgc gccacttcag ccaaccccac gcggcgtaga
     2761 gcatcgccca ccagtcggag ctgaactcgc tgtccatgaa gcggatgcac tccaccggca
     2821 ggtcccagcg gtgctcgtgc gccgcgcgga tcgccggctg cacgtcggag aggaactcgt
     2881 tgacggtctc ggcgatctgc tcgcgcgtga tgccctcggc gagcgtcgcg cccggcggcg
     2941 ccgcggggct cgcggcctcc caggcgagcg gcgcgcggct gttcgtgtcc agcgcgagca
     3001 gctcgaagag gatcgaggtg ccggtgcgcc cctgccccgc gatcagcagc ggcgcgacga
     3061 cctgctcatg gcgtatcgcc ggctgcgcgc ggcaggcctc ggtgaccagc aggcggttgc
     3121 gcaggccgcg cagcaactcg gcgcgcgtca ctagccgccc gatcgtgtgc aggtccacct
     3181 cggtctgcag cgattcgacc agcgcgcgaa aggcgtcctc ccagcccggc tcgccgaaat
     3241 ccgccaggcc ggtgctcgcg cgcgcctcct cgatcagctc ctctggcgcg aggctcacca
     3301 gccggcgcga gccacccacc atcgccccga ggtagttcag tcgccggacc cagtcgggtt
     3361 gctcgatatc catgtctccc ccctgccgaa agccggatgc aatgccggcg gcagtgtagc
     3421 gccctcttcc cggtcagggc atgaagagaa cgtaacgcct cagccgcctc gacccggttg
     3481 actcggcccg ggcgcgctga gaccctgcgc gacacgacgc agaaaagaca ggagccccga
     3541 tgaccgagtc acgccgtgca ttccgcgagc ttctcgaact gctcggccag atcgacgcgc
     3601 agtacgtcgg ggagaccggc gaatacaaat cggcgctcga cgtcgccgac ggccaccgct
     3661 tcatcctgca cgggctgcgc cgcgcgctcg gctcgcaact cgaggccgac acgcgccgcc
     3721 cggtgttcca gcgcgcggtc accccgacga cgaagttcgg cggcgacagc cccgatgcga
     3781 tctaccacga gtgcaacgtg agcgccgatt gcagctaccg catccgcggc gacatggccg
     3841 gcgcggtcta cgtatcgctg tcggtgcagt ccggcggcgg cgaagccgag ggcgtaggcg
     3901 ccagcatcaa cagcgaacag ttcgaggtgc ggcccgatgg ctccttcgag atcctgctct
     3961 cgcgcgcgcc gcccgccgac tcacgcaact gggtgcggat gcccgagggc gcgctcaacg
     4021 tgctgacgcg gcactacttc gaggaggccg gcaacgtgat ggccgacacc gcgcgacggc
     4081 tggacctcga gatcgacgcg ctcggcgact tccccgctcc gcgcgaaccc accgtcgaga
     4141 gcatcaccgc gggcatcgag cgcgtttcgc gctggatgcg cagccgcacg ctggaggaat
     4201 cgaagggccg cacgacgatg cccgcctggg tttccgtcgt gccgaaccgc ttcaacgagc
     4261 ccgagaagcc cggcgccgac atggcctatg ccgcgctcga catcgcctac gcgatggcgc
     4321 cctttcacgt gccgccgggc aaggcgctgg tgataagcgg ccgcttcccc gcctgccgct
     4381 acgcgggcgt ggtgctgtgg agccgcttcc ggcagagctt cgattacatg aaccggcgcg
     4441 tctcgctgaa ccgcgcgcag accgtcacgg acggtgcagg gaattaccgc atcgtgctgg
     4501 ccgagcgcga ccccggcgta cccaactggc tggacaccgg cggccgcgcg agcggattca
     4561 tgtactggcg cttcctgttg cccgaggagc ccatcacggc gccgcagtgc gagctggtgg
     4621 atatcgattc tctgctcagg tgacgcgctg gcgggctcat ccgctcgcgc cgagcgcgtg
     4681 gtagagcggc tccagcagcg catcggtgcg cgcgctctcg aaacgcttgc cgggccggtt
     4741 ggtcacgtag gcgaaggcga caccgctcgc gggatcggca aagcccaggc tgccgccgta
     4801 gccgaagtgc ccgtagccgc gctcgctgcc gccgatgcgc cggcccggcc gcgacaactg
     4861 gaaacccagc ccgaagcgcg aatgcttgcc gagcacgcgg tccggaccat ccgactggat
     4921 actgcacgcc tgcgcgcgca accccgggcc cacgatgccg ccggcggccg cgtcgcgatg
     4981 catgaacacg tcgtagagca gcgccaccgc acgcgccgtg gcgtgcgcgt tcgtcgaggg
     5041 aatcgccgcg ctgcgccatt ggggccggtt gacgatgccg aagccggaga tgcccggcgg
     5101 gttgaagtac acgctggcca gcatcgcgtc ggtcgccgga tcgcccgagg cgagatgcgc
     5161 gcgcacggcc gattgcccat cggtgatcgg ggcggcgtcc gcctccacga tcgcggccac
     5221 gcgcgcgtgt tcgcgcagtg gcagcccgac gtggtagtcc gcctccagcg ggcccgtgag
     5281 gcgttcgcgc agggcggcgg cgaagggcat cccgagccgc ctcaccacgg gctcgccgat
     5341 caggaatccg tgcgtgttga cgtggtagcc gtgcgccgca tcgggctccc agtacggtgc
     5401 ctgcgccgac agttccaccg tcatcgtcga ccagtcgtac atggcatcgg acggcaacgg
     5461 acgccgcacg ccgggcaatc cgccgcgatg cgcgagcagc atgcgcagcg tcagccgctc
     5521 cttgccagcg gcggcgatct cgggccatat ctcgcgcagc ggacgatcga gcgcgagctc
     5581 gccgcgctcg acaaggccga gcaccagcat cgaggcgatg cccttgccca cggagtagat
     5641 gttgaccagc gtatcgcgct gccaggtctc ggtgcggccc gcgtcgcgat ggccgcccca
     5701 caggtcgatg accacgcgcc cgccgatccg cacgcaggcc gcggcaccga gttcgttacg
     5761 cgtgcggaaa ttctcgcgga aggcgtgttc tacttccgcg aaggcggggt cgcagaatcc
     5821 ggcgacgggc acatcgtcgg tacgggggtg atcggtgttc gggggattcg acatggcggg
     5881 tgcctcgtgt gcgtacatac
//
LOCUS       DAORUQ010000186         5797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_16526_length_5797_cov_5.611459, whole genome shotgun sequence.
ACCESSION   DAORUQ010000186 DAORUQ010000000
VERSION     DAORUQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_5797_cov_5.611459"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..798
                     /locus_tag="PLW13_13380"
     CDS             151..798
                     /locus_tag="PLW13_13380"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71420.1"
                     /translation="MPGMFNRIVARLARRLMARREADVVIRRAAQMHRVRLMGAHATT
                     LFITGPKFRNWGFMTDRGWVHWSEYLGPLQPDRSSRCSTVFERMLVDDGILLFKYWLS
                     VDQQYQEERFAERAQDPLKRWKLSPIDLQSRASYEDYGRARDAMFEATHTRHAPWCVV
                     DFNDQKRGRLNLIRNLLDRVPDCQLPEEPFDLPPLAHKPRKERFTTALKPLRNRF"
     gene            867..2687
                     /locus_tag="PLW13_13385"
     CDS             867..2687
                     /locus_tag="PLW13_13385"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQY71421.1"
                     /translation="MQAGEWVRGASISLALVFGVAGAAGVLADAKADNAAALRQIFAD
                     SDEAALELDPLAAMMRGDMRYAAQFGDYITPGYYARMKANTASEMKRLDAIDRAALSR
                     EDRIAYDVFRYQGLQLLDFYERGIIAIQARLPIDHFSGEHVFFPDICSGRSVAPFDTV
                     QDYDNGLARLEGYALYLERSIGRMREGMATGHTQPRLVMENILDQLARQMAGGVEASP
                     FYQPINDMPGDFAATDRERLARAYRAVIHERVFGAYRELQDFIRTEYLAASRDAKPGL
                     VSMKDGPALYKALIEQNTTTDMSAEEIHRIGLDEVARIRHDMLAIKDRVGFKGTLKEF
                     FGHLRSDPQFKFASKQALLDGYNAIRAEVDAAVPKLFSLTPRSPFEIRPVPGYSEENE
                     AAGSYRQGTPDGTRPGIFFVNTYDLPSRTTPGMETLFLHEAIPGHHFQISLAQENEAL
                     PNFLRFGGNTAFVEGWALYAESLGPELGMFTDPYQAFGSLDDEMLRAMRLVVDTGIHA
                     MGWTREQAIQYMLDNSAQSETEARTEVERYIAIPGQALAYKVGALTIRRLRTEAEQAL
                     GDRFDPREFHAQVLDTGALPLAVLEAKIRDWIKAKQAAAA"
     gene            2991..4193
                     /locus_tag="PLW13_13390"
     CDS             2991..4193
                     /locus_tag="PLW13_13390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HQY71422.1"
                     /translation="MFEQLARLDPDPILGMLAAYRADPRAHKIDLGVGVYQDELGRTP
                     IMRAVSDAERHLLVTETTKTYQGIAGDPVFNERLLALLLGASHRVIGEDRVRGVQTPG
                     GCGALRIGAELIQRARRSAKVWVSTPTWANHIPLIGGAGLQLAEYPYFDAASGGIDFD
                     AMMDTLSRVGPGDLVLLHGCCHNPTGADLDLGQWRAVTELALNNGFTPYIDVAYQGLA
                     DGVDADVAGVRHMLSAVPEAVVASSCSKNFGLYRERVGAVYFIGATRAQADAVQTQAM
                     AAARQIYSMPPAHGAAIVSTILGDATLRADWLEELGEVRGRINAMRTLLAARLEGNAA
                     GKDFSFITRQKGMFSYFGITPQQVTRLREEFAVYMMESTRINLAGITPANIDALASAV
                     LAVLEPAA"
     gene            4212..>5797
                     /locus_tag="PLW13_13395"
     CDS             4212..>5797
                     /locus_tag="PLW13_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQY71423.1"
                     /translation="MTTSAAAVAGSATLQRDVRRPVWLFSLDSEQFHAAPTATGGLIA
                     WYRAWGATPDGIDFRQLHFREREDVAAWSARELGALAGEAAAALARGLEPVAGISFYT
                     WNAAEFLELVRGLRAACPGLAIVAGGPHVQQAESYLGVDPIDVIVLGEGEATFQELLD
                     TPRAGWPRVPGIAWLDHAGRVATSAPRERQRDLTRIPSPFDVLELTDAAGRPRYDAVT
                     YETTRGCPYKCAFCEWGTGAIGTKMIGFPMERLRRDWQKIVRAGIPNIWLADSNFGAL
                     KDDVEKARLICELKEQHGLPRTFATSWSKKHSPRVQQIVLMLHEHGLLPFYQLALQTL
                     TPRALELCHRENMAANEYEPIAQRMAQAGIPISAELIWGLPGDDLASFERNLDRLLAT
                     FPNINIFGYTLLPGTEFYERREEYRIETLPVAGYGKAHGEYVVGCHTFARAEGEEGYF
                     LITAHVILLSGYVLAHTARWLALATDVPVGRMLRVVLDALLQAFAAQLPGLDAADRMR
                     VYEARARIYLAVLADPSRAQA"
BASE COUNT          917 a         1962 c         2012 g          906 t
ORIGIN      
        1 tcagcggctc ccgtatcgcc gtcgccgcga cacgatggct gaggatctgc atctgcgggc
       61 tctggcgcgg gtcctggtca gcccgggcgt ggcgggaggt gcgagtagga tactatgctc
      121 caaagtgccg gactcggccg gatggagaag atgcctggaa tgttcaaccg cattgtcgcg
      181 cgcctggcgc gccggctcat ggcccgacgc gaggccgatg tcgtgatccg ccgcgccgcg
      241 cagatgcatc gcgtccgcct catgggcgcg cacgccacga cgctgttcat taccggcccg
      301 aagttccgca actggggctt catgacggat cgtggctggg tgcactggag cgagtacctc
      361 ggaccgttgc aacccgacag gagcagccga tgcagcacgg tgttcgagcg catgctggtc
      421 gatgacggca tcctgctctt caagtactgg ctgagcgtgg accagcagta ccaggaggag
      481 cggttcgccg agcgcgcaca ggatccgctc aagcggtgga aactgtcgcc catcgatctg
      541 cagtcgcgtg cgagctacga ggattacggc cgcgcgcgcg atgcgatgtt cgaggccacg
      601 cacacgcgcc acgccccctg gtgcgtggtg gatttcaatg accagaagcg cggccgcctc
      661 aacctgatcc gcaacctgct cgaccgcgtg cccgactgcc agttgccgga ggagccgttc
      721 gatctgccgc cgctggcgca caagccgcgc aaggagcgtt tcacgacggc cttgaagccg
      781 ctgcgcaacc gcttctgatc atgcggccag cgcggatgcg ccaatgcgcg ggtgacagtt
      841 tgcttcgatc cgtgacgggg ggattcatgc aggcagggga atgggtgcgc ggtgcctcga
      901 tatcgctcgc gctggtgttc ggcgtggccg gcgctgccgg cgtgctcgcg gatgcgaagg
      961 ccgacaacgc cgccgcgctg aggcagatct tcgccgacag cgacgaggcg gccctggaac
     1021 tggatcccct ggctgccatg atgcgtggcg acatgcgtta tgcagcgcag ttcggggact
     1081 acatcacgcc cggatactac gcgcggatga aggcgaatac cgcgagcgaa atgaagcgcc
     1141 tcgatgcgat cgaccgcgcc gcgctgtcgc gcgaggaccg gatcgcctac gacgtgttcc
     1201 gctaccaggg cctccagctg ctcgatttct acgagcgcgg catcatcgcg atccaggcgc
     1261 ggctgcccat cgaccacttc agtggcgagc acgtgttctt ccccgacatc tgctcgggca
     1321 ggtcggtcgc gccgttcgac acggtgcagg actacgacaa cggcctggcg cgcctggagg
     1381 gctacgcgtt gtacctcgag cgcagcatcg ggcgcatgcg cgagggcatg gccaccggtc
     1441 acacccagcc caggctggtg atggagaaca tcctcgacca gctcgcgcgg cagatggccg
     1501 gcggcgtcga ggccagcccg ttctaccagc cgatcaacga catgccgggc gatttcgccg
     1561 ccaccgaccg ggaacgcctt gcccgcgcgt accgcgccgt gatccacgag cgcgtgttcg
     1621 gcgcctaccg cgagctgcag gacttcatca ggaccgaata cctcgccgcc agccgtgacg
     1681 cgaagcccgg gctggtctcg atgaaggacg gtcccgcgct gtacaaggcg ctgatcgagc
     1741 agaacaccac caccgacatg agcgccgagg agatccaccg gatcggcctc gacgaggtcg
     1801 cgcgcatcag gcacgacatg ctcgcgatca aggacagggt gggtttcaag ggcacgctga
     1861 aggagttctt cggtcacctg cgcagcgatc cgcagttcaa gtttgccagc aagcaggcgc
     1921 tgctcgatgg ctacaacgcg atccgcgccg aggtggatgc cgccgtgccg aagctgttct
     1981 cgctgacgcc gcgatcgccc ttcgagatcc gtcccgtgcc cgggtactcg gaggagaacg
     2041 aggccgccgg ctcctatcgc cagggcacgc cggacggcac gcgcccgggc atcttcttcg
     2101 tgaataccta cgacctgccc tcgcgcacca cgcccgggat ggagacgctg ttcctgcacg
     2161 aggcgatccc gggacaccat ttccagatca gcctggcgca ggagaacgag gcgctgccga
     2221 acttcctgcg ctttggcggc aacacggcct tcgtggaagg ctgggcgctg tatgccgagt
     2281 cgctgggacc ggagctcggc atgttcaccg atccctacca ggccttcggc agcctcgacg
     2341 acgagatgct gcgcgcgatg cggctggtgg tcgacaccgg catccatgcc atgggctgga
     2401 cccgcgagca ggcgatccag tacatgctcg acaattcggc gcagtccgaa accgaggcgc
     2461 gcaccgaggt cgagcgctac atcgcgatcc cgggccaggc gctcgcctac aaggtcggtg
     2521 cgctcacgat ccggcgcctg cgcaccgagg ccgagcaggc cctcggcgat cgcttcgacc
     2581 cgcgcgagtt ccacgcccag gtgctcgata ccggcgccct gccgctggcg gtgctcgagg
     2641 ccaagatccg cgactggatc aaggccaagc aggctgccgc ggcctgagga tacggatcgc
     2701 gggcgtggcc cgctcctaca ccagaatcga cgcgtcaacg ataggcgcgc ccaggggccg
     2761 ccccgctgtg cgagcggaca tgtggatgcc gaaatcgccg ggagcgattt cggcaacacg
     2821 cgtagcggcc gcacatggac gtgccaggcc gcggccgcga gtgcagcggg gcggcccctg
     2881 ggcgtgccgg gtcggtgctg tctttggtcc ggctcgacgc cgcgccggtg acggggcaca
     2941 atcgctgctc attgcggcca tgaccgcctc aagcgataac cggagacccg atgttcgagc
     3001 agcttgcccg cctggatccc gatcccatcc tcggcatgct ggccgcctac cgcgccgatc
     3061 cgcgcgcgca caagatcgat ctgggcgtcg gcgtctacca ggacgagctc gggcgcaccc
     3121 cgatcatgcg cgcggtgagc gacgccgagc gtcacctgct ggtcaccgag accaccaaga
     3181 cctaccaggg catcgccggc gatcccgtgt tcaacgagcg cctgctcgca ctgctgttgg
     3241 gcgcgagcca ccgcgtgatc ggggaggatc gcgtgcgcgg cgtgcagacg ccgggcggct
     3301 gcggggcgtt gcgcatcggg gccgagctga tccagcgcgc gcggcgctcc gcgaaggtct
     3361 gggtcagcac gccgacctgg gccaaccaca tcccgctgat cggcggggcc ggtctgcagc
     3421 tggccgagta cccgtacttc gacgccgcga gtggcggcat cgatttcgac gcgatgatgg
     3481 acacgctgtc gcgggtgggc cccggcgacc tggtgctgct gcacggctgc tgccacaacc
     3541 cgaccggcgc cgacctggat ctcgggcagt ggcgcgccgt taccgagctg gcgctgaaca
     3601 acggcttcac gccgtatatc gacgtcgcct accagggtct cgccgacggc gtcgacgcag
     3661 acgtggccgg cgtgcgccac atgctctccg ccgtgcccga ggccgtggtc gcgagttcgt
     3721 gctcgaagaa tttcgggctc taccgcgagc gcgtgggcgc ggtgtacttc attggcgcga
     3781 cgcgcgccca ggccgatgcc gtgcagaccc aggcgatggc ggccgcgcgc cagatctact
     3841 cgatgccgcc ggcgcacggc gcggcgatcg tgtccacgat ccttggcgat gcgacgctca
     3901 gggccgactg gctggaggaa ctcggcgagg tgcgaggacg catcaacgcg atgcgcacgc
     3961 tgctggccgc gcgcctggag ggcaacgccg ccggcaagga tttcagcttc atcacgcggc
     4021 agaaggggat gttttcgtac ttcggcatca cgccgcagca ggtcacgcgg ctgcgcgagg
     4081 agtttgccgt ttacatgatg gagtcgacgc gcatcaacct ggccggtatc acgcccgcga
     4141 acatcgacgc tctcgcgagc gcggtgctcg cggtgctaga gcccgcagcc tgagtcccgc
     4201 ggccagcgcc gatgacgacc tcggcagccg ccgtggcagg gtccgccacg ctgcagcgtg
     4261 atgtccggcg tccggtgtgg ctgttcagtc tcgattccga gcagtttcac gccgcgccca
     4321 ccgccaccgg cgggctgatc gcgtggtacc gcgcctgggg tgcgacgccc gacggcattg
     4381 atttccgcca gctgcatttc cgcgagcgcg aggacgtggc ggcgtggagc gcgcgcgaac
     4441 tcggcgcgct ggccggggag gccgccgctg cactggcgcg cgggctggag ccggttgcgg
     4501 gcatcagctt ctacacctgg aacgcggcgg aattcctcga gctcgtgcgc ggcctgcgcg
     4561 ccgcgtgtcc ggggctcgcg atcgtcgcgg gcggcccgca cgtgcagcag gccgagagtt
     4621 acctgggtgt cgaccccatc gacgtgatcg tgctgggcga gggggaggcc acgttccagg
     4681 agttgctcga cacgccgcgc gccggttggc cgcgcgtgcc cggcatcgcg tggctggacc
     4741 atgcgggcag ggtggcgaca tcggcgccgc gcgaacgtca gcgcgacctc acgcgcatcc
     4801 cttcgccctt cgacgtgctc gaactcaccg acgccgcggg gcgcccgcgc tacgacgccg
     4861 tgacctacga gaccacgcgc ggctgcccct acaagtgcgc cttctgcgag tggggcaccg
     4921 gcgcgatcgg cacgaagatg atcggttttc cgatggagcg gctgcgccgc gactggcaga
     4981 agatcgtgcg cgccggcatc ccgaacatct ggctggcgga ttcgaacttc ggcgcgctga
     5041 aggacgatgt cgagaaggcg cggctgatct gcgagctcaa ggagcagcac ggtctgccgc
     5101 ggaccttcgc gacctcgtgg tcgaagaagc acagcccgcg cgtgcagcag atcgtgctga
     5161 tgctgcacga gcacgggctg ttgccgttct accagctcgc gctgcagact ctgaccccgc
     5221 gcgcgctcga gttgtgccat cgcgagaaca tggcggcgaa cgagtacgaa ccgatcgcgc
     5281 agcgtatggc gcaggccggc atcccgatct cggccgaact gatctggggg ttgccggggg
     5341 acgatctcgc gagcttcgag cgtaacctcg accgcctgct cgcgaccttc ccgaacatca
     5401 acatcttcgg ctacacgctg ctgcccggca ccgagttcta cgagcggcgc gaggaatatc
     5461 gcatcgaaac gctgcccgtg gcgggctacg gcaaggcgca cggcgagtac gtggtgggct
     5521 gccacacctt tgcgcgcgcc gagggcgagg agggttactt cctgatcacg gcgcacgtga
     5581 tcctgctcag cggctacgtg ctggcgcaca ccgcgcgctg gctcgcgctg gcgaccgatg
     5641 tgcccgtggg gcggatgttg cgcgtggtgc tcgatgcgct gctgcaggct tttgccgcgc
     5701 agttgcccgg gctcgatgcc gccgatcgca tgcgggtcta cgaggcgcgc gcgcggatct
     5761 acctcgcggt gctcgcggac ccctcgcgtg cgcaggc
//
LOCUS       DAORUQ010000187         5755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_16709_length_5755_cov_6.924561, whole genome shotgun sequence.
ACCESSION   DAORUQ010000187 DAORUQ010000000
VERSION     DAORUQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16709_length_5755_cov_6.924561"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLW13_13400"
     CDS             complement(<1..731)
                     /locus_tag="PLW13_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY71424.1"
                     /translation="MTDIEFENRDGVGLITFARPETLNVFSPGMMAGLGDLYRHCDAD
                     ESVRVVVVTGRGTAFCAGADLSGGEVFAAHGELDFSSCPLSMQAWELRKPVIAACNGH
                     AIGVGLGIAVQCDMRIVARGAKYGFLQNRRGVVADFGVEWLLPRLIGFERAFEMLVRG
                     SRIDGAEAAAWRLAGRAAEAQEVLPLALEIADDMVRNCSPLVMGMHKRLLWRALETGR
                     DDFVALETRALHHSMRQPDALEGGMA"
     gene            complement(767..1306)
                     /locus_tag="PLW13_13405"
     CDS             complement(767..1306)
                     /locus_tag="PLW13_13405"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HQY71425.1"
                     /translation="MEPGLLIVASGAIALSWLMAVAGGAKLRHQRDHARALDAYALLP
                     EGAGRWLVPVLGAVECAAALAIWPRGTRAAAALLILALLVLYSGAIAINLLRGRRDIA
                     CGCGGAATHVPLSGWLLARNAALMAAALALHEAPQALALAGASWPSALAALAVATLLV
                     LLYNGANFLLSRDTLLQDD"
     gene            1387..2622
                     /locus_tag="PLW13_13410"
     CDS             1387..2622
                     /locus_tag="PLW13_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQY71426.1"
                     /translation="MSTAPSPFDPVAIGPLQLRNRFIKSGANEAMCLDGAPTRALVQH
                     HRDLAAGGVALTTVAYVAVSEVGRTLPNQIWMRPGILPDLRVLTDAVHAEGGAISAQI
                     THGGSFVTGVKVKGATMSSVSGLNKAGLLAGNWRRRAMTAADMDLVTQQFVDAAKLAR
                     EAGFDAVELHMGHGYLLNQFISPLDNTRTDEYGGNAENRVRFPARVLAAVKEAVGADL
                     AVIAKINVADGPRRGADADDAVVTARALERAGADLLVLSGGRNVESTWFMFGSLVDQD
                     EMARMLGGDPLSRLAIRLSSLTGPRSMTFREMYFLEHSRKVRAAVTMPLAYLGGVKSL
                     DNAERAMSEGFDCVVMARALIHDTGLVNRFRSGETRQSGCTSCNRCVAYIYHPAGTRC
                     VENAPNDPALNLVRAAAVD"
     gene            complement(2719..3012)
                     /locus_tag="PLW13_13415"
     CDS             complement(2719..3012)
                     /locus_tag="PLW13_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HQY71427.1"
                     /translation="MAEKKPAKKPAAKKAAAKKPAAAKKPAAKKAVAKKPAAAKKPAA
                     KKAAAKKPAAKKAVAKKPAAAKKPAAKKAAAKKPAAKKPAAKKPAAKKAAAKK"
     gene            complement(3128..3529)
                     /locus_tag="PLW13_13420"
     CDS             complement(3128..3529)
                     /locus_tag="PLW13_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HQY71428.1"
                     /translation="MSSFGPDSDDSMALPFWSAAREGRLVLPYDRASGCCTWYPHAGS
                     DIEWREVRGPATLVAWSVVRAPINPEFRAPYAPALVSLDCEPHVRLVTQIVDCDFADL
                     RCDMPLELCFRTLEPIRRAPFRAPVFRPASN"
     gene            complement(3532..4779)
                     /locus_tag="PLW13_13425"
     CDS             complement(3532..4779)
                     /locus_tag="PLW13_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY71429.1"
                     /translation="MGVQSIGQLRQRAGSFDWGRAYEIPDVRGEVAIVGVGESQYSGP
                     SGRTAKEMALVAVEKAIADAGLAPADIDGLMSTANVADQLMPEDFHAHFGTSHPMWYS
                     GEGGGMVWAATCPEQAAEALRARKARHIINVFSVDWASRRAAGTGTPGDYHANEPMKA
                     LFEVPFGWYPQPVYMATQACRHMHDYGTTPEQLGELAVTFRRHANSHPGAVMRAKTLS
                     MEQYLAKPMLADPLRMEDCCLVSDGAAAFVMSTPERARDLPHAPVIVEGIGRGVIHNA
                     PYISQQKVLTATPQTFAAPWAFAMADITPAEVDVIALYDCFSITALMMIEDMGFCAKG
                     EGGAFVSGGRLGFDRPRRSGGLPCNTHGGLMSHAYVLGIAHVVEIVRQLRGEAANQVS
                     DARVGLYGGFTAEEAATLILRRA"
     gene            4914..>5755
                     /locus_tag="PLW13_13430"
     CDS             4914..>5755
                     /locus_tag="PLW13_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71430.1"
                     /translation="MNDKPSSRAILPGYWFSQNPDKAWGEKFFLTFIPFWFVYNIVVQ
                     QMGWLDTGNFWNITQNLLMWLPYCVLLPWFLRRNSGIAWHRSYWFKFNVFMFWWIVLA
                     TYFHTEYFFEVLGMRYRFPEVTLYLDSALVGPDEATALGAHMKVPPSMYFNATAFFIV
                     YHTSAVILMRRIRTMTLSWAPLARGLAWAVIVGAVSLFWGWGETAFYFKLAPNDFSNV
                     WYEDLDRMLAYGSYFYALYFIVAFPIVYRLDEAAEGERWSLGRVIIEASCVGMLTLLL
                     IDLAT"
BASE COUNT          921 a         2037 c         1857 g          940 t
ORIGIN      
        1 gccatgccgc cctccagcgc atcgggctgg cgcatgctgt ggtgcagcgc gcgcgtctcc
       61 agcgcgacga agtcatcgcg ccccgtctcc agcgctcgcc acagcaggcg cttgtgcatg
      121 cccatcacca gcggcgagca gttgcgcacc atgtcatcgg cgatctccag cgcgagcggc
      181 aaaacctcct gcgcctcggc cgcgcgcccg gcgaggcgcc aggccgccgc ctccgccccg
      241 tcgatgcgcg agccgcgcac cagcatctcg aaggcacgct cgaaaccgat cagccgcggc
      301 agcagccact cgaccccgaa atcggccacc acgccccggc ggttctgcag gaagccgtac
      361 ttcgcgccgc gcgccacgat gcgcatgtcg cattgcaccg cgataccgag cccgacgccg
      421 atggcgtgcc cgttgcaggc ggcgatcacc ggtttgcgca attcccaggc ctgcatggac
      481 agcgggcagg aactgaagtc cagctcgccg tgcgccgcga acacctcgcc gcccgagagg
      541 tcggccccgg cgcagaacgc cgtgccgcgc cccgtcacca ccaccacacg cacggactcg
      601 tcggcgtcgc agtgccgata cagatcgccc agcccggcca tcatcccggg gctgaacacg
      661 ttcagcgttt cgggtcgtgc gaaggtgatg aggcccacgc cgtcgcgatt ctcgaactcg
      721 atatcggtca tgcgtcctcc gaagatgcga cgccggcacg cgacattcag tcgtcctgca
      781 gcagggtgtc gcgggaaagc agaaagttcg cgccattgta gagcagcacc agcagcgtcg
      841 cgaccgcgag cgccgccagc gccgacggcc atgatgcgcc ggcaagcgcc agggcctgcg
      901 gcgcctcgtg cagcgcgagc gcggcagcca tcaaggcggc gttgcgcgcg agcagccagc
      961 cgctcagcgg cacgtgggtc gccgcgccgc cgcaaccgca cgcgatgtca cggcgtccgc
     1021 gcagcaggtt gatcgcgatg gccccggagt acagcaccag cagcgcgagt atcagcaagg
     1081 ccgccgccgc gcgcgttccc cgcggccaga tggcgagcgc ggcggcgcac tctacggcgc
     1141 ccagcaccgg cacgagccag cgccccgcac cctcgggcag cagcgcgtag gcatcgaggg
     1201 cgcgcgcatg atcccgctga tggcgcagtt tggcgccacc ggccacggcc atcagccacg
     1261 acagcgcgat cgcgcccgac gcgacgatca gcaatcccgg ttccattcgc ccgcccccga
     1321 cgcgtcttgc aaagtcacta tgcccgcccg atcatgcgcg cgccgcttcc caggagaatc
     1381 acccgcatga gcaccgcgcc ttccccattc gatcccgtcg ccatcggacc cttgcagttg
     1441 cgcaaccgtt tcatcaagtc cggcgccaac gaagccatgt gcctggacgg cgcgcccacg
     1501 cgcgcgttgg tgcagcacca tcgcgatctt gccgcaggcg gcgtcgcgct gacaacggtg
     1561 gcctacgtgg cggtgagcga agtcggccgc acgctgccca accagatctg gatgcgcccc
     1621 ggaatcctgc ccgacctgcg cgtgctcacc gacgcagtgc acgccgaagg cggcgcgatc
     1681 tcggcgcaga tcacgcacgg cggcagcttc gtcaccggcg tgaaggtcaa gggcgcgacg
     1741 atgagctcgg tctcgggcct caacaaggcg ggcctgctcg cgggcaactg gcgccggcgc
     1801 gcgatgacgg cggccgacat ggacctggtg acgcagcagt tcgtcgacgc cgcgaaactc
     1861 gcgcgagagg cgggcttcga cgccgtagaa ctgcacatgg ggcacggcta cctgctgaac
     1921 cagttcatct caccgctcga caacacgcgc accgacgaat acgggggcaa cgcggaaaac
     1981 cgcgtgcgtt tcccggcgcg ggtactggcc gcggtgaagg aagcggtggg cgccgatctg
     2041 gcggtgatcg ccaagatcaa cgtcgcggac gggcccaggc gtggtgccga cgccgacgat
     2101 gccgtggtca cggcgcgcgc gctggagcgc gccggcgccg atctgctggt gctctcgggc
     2161 ggacgcaacg tggagtcgac ctggttcatg ttcggcagcc tcgtcgacca ggacgagatg
     2221 gctcgcatgc tcggcggcga tccgctgtcg cgcctggcga tcaggctgag ctcgctcacc
     2281 ggtccgcgct cgatgacgtt tcgcgagatg tacttcctgg agcactcgcg caaggtgcgc
     2341 gccgcggtga cgatgccgct ggcctacctg ggcggcgtga agtcgctgga caacgccgag
     2401 cgcgcgatga gcgagggctt cgactgcgtg gtgatggcac gcgcgctgat ccacgacaca
     2461 ggactggtga acaggttcag gagcggggaa acccggcagt cgggctgcac gagctgcaac
     2521 cgctgcgtcg cctacatcta ccaccccgcg gggacgcgct gcgtggaaaa tgcgccgaat
     2581 gatcccgcgc tcaacctggt gcgtgctgcg gcggtggact gaggaggagg aactctgccg
     2641 tgcgggcggc gaacgcaggc cgcccgacgc ggcggaagct gcccgacacg tccgtgtgca
     2701 tcgggcgcag gggaacaact acttcttcgc tgcggctttc ttcgcggcgg gcttcttcgc
     2761 tgccggcttc ttcgctgccg gcttcttcgc tgcggccttc ttcgcggcgg gcttcttcgc
     2821 tgccgcgggc ttcttcgcta cggccttttt cgcagccggc ttcttcgcgg cggccttctt
     2881 ggcggcgggc ttctttgctg ccgcaggctt cttcgccacg gcctttttcg cagccggctt
     2941 cttcgctgcg gcgggtttct tggctgcggc cttcttggct gcaggcttct tcgccggttt
     3001 cttctctgcc atcggtagtc tcccgttgct gatggttggg aatccacgaa gattccactc
     3061 actgtctata gcacatgcca caccagcgcg cgggtgaatt ttgcgcaaac gagcgcgcgc
     3121 cgaatagtca attcgatgcc ggacgaaaca ccggcgcgcg gaaaggcgca cgcctgatcg
     3181 gctcgagcgt gcgaaagcaa agctccagcg gcatgtcgca gcgcagatcc gcgaagtcgc
     3241 agtcgacgat ctgcgtcacc agccgcacgt gcggctcgca atcgaggctc accagcgccg
     3301 gtgcgtaggg cgccctgaat tcggggttga tcggcgcgcg cacgacggac cacgccacca
     3361 gcgtcgccgg gcctcgcacc tcgcgccact cgatgtccga gccggcgtgc ggataccacg
     3421 tgcaacaccc gctggcgcga tcgtagggca gcaccagcct gccctcgcgc gcggcactcc
     3481 agaacggcaa ggccattgag tcatcgctgt cgggaccgaa actggacatg ctcaggctct
     3541 ccgcaaaatc agtgtggccg cctcttcggc ggtgaatcct ccgtacagcc cgacgcgggc
     3601 atcgctcacc tggttcgcgg cctcgccgcg cagctggcgc acgatctcta ccacgtgcgc
     3661 gatgccgagc acgtaggcgt gcgacatcag tccgccgtgc gtgttgcacg gcagcccacc
     3721 gctgcggcgc gggcggtcga aaccgagccg cccgccggag acgaaggcac caccctcgcc
     3781 cttggcgcag aagcccatgt cctcgatcat catcagcgcc gtgatcgaga agcagtcgta
     3841 cagcgcgatc acgtccacct ctgccggcgt gatgtcggcc atcgcgaagg cccacggcgc
     3901 ggcgaaggtc tgcggcgtcg ccgtgagcac cttctgctgc gagatgtagg gcgcgttgtg
     3961 gatcacgccg cgaccgatgc cctcgacgat gaccggcgca tgcggcaggt cacgcgcgcg
     4021 ctccggcgtg ctcatcacga aggccgccgc gccatccgag accaggcagc agtcctccat
     4081 gcgcagcgga tccgcgagca tgggcttggc gaggtactgc tccatgctca gtgtcttcgc
     4141 gcgcatcacg gcaccgggat ggctgttggc gtggcgacgg aaggtcaccg cgagttcgcc
     4201 gagctgctcc ggcgtggtgc cgtagtcgtg catgtggcgg caggcctgcg tcgccatgta
     4261 caccggctgc ggataccagc cgaagggcac ctcgaacagc gccttcatcg gctcgttggc
     4321 atggtagtcc ccgggcgtgc cggtccccgc ggcgcggcgc gaggcccagt caaccgagaa
     4381 gacgttgatg atgtgccgcg ccttgcgcgc gcgcagcgcc tcggcggcct gctctgggca
     4441 ggtcgcggcc cagaccatgc cgccgccctc gccgctgtac cacatcgggt gcgaggtacc
     4501 gaagtgtgcg tggaagtcct caggcatcag ctggtccgcg acgttggcgg tggacatcag
     4561 cccgtcgatg tccgccggcg cgagaccggc atccgcgatg gccttctcga cggcgacgag
     4621 ggccatctcc ttcgcggtgc ggcccgaagg accgctgtac tgcgattcgc cgactccgac
     4681 gatcgcaacc tcgccgcgca cgtccgggat ctcgtaggct ctgccccagt cgaaactccc
     4741 ggcacgctgg cgcaattgcc cgatactctg aacacccatc cgctcgctcc ctggtccggc
     4801 acgatccacc gcggcccgca ccgtgcgcgc cgcccccaaa aaacatttgc agtatactgt
     4861 tttattctgg aaactcacca cacgtcaaca acacgcccgg aagagcccgc accatgaacg
     4921 acaagccctc ctcgcgcgcc atcctgcccg gttactggtt ctcgcagaat cccgacaagg
     4981 cctggggcga gaagttcttc ctgaccttca tcccgttctg gttcgtctac aacatcgtgg
     5041 tgcagcagat gggctggctc gataccggca acttctggaa catcacgcag aacctgctga
     5101 tgtggctgcc ctactgcgtg ctgctgccct ggttcctcag gcgcaactcc ggcatcgcct
     5161 ggcaccgcag ctactggttc aagttcaacg tgttcatgtt ctggtggatc gttctcgcca
     5221 cctatttcca caccgagtac ttcttcgagg tgctcggcat gcgctaccgc ttcccggagg
     5281 taacgctgta cctggactcc gcgctggtcg gccccgacga ggccacggct ctcggcgcgc
     5341 acatgaaagt gccgccctcg atgtacttca acgcgaccgc gttcttcatc gtgtaccaca
     5401 ccagcgccgt gatcctgatg cgccgcatcc gcaccatgac gctgtcgtgg gcgccgctcg
     5461 cgcgcggact ggcatgggcg gtgatcgtgg gcgcggtgtc gctgttctgg ggctggggcg
     5521 aaacggcttt ctacttcaag ctcgcgccca acgacttctc gaacgtgtgg tacgaggacc
     5581 tcgatcgcat gctggcctac ggctcgtact tctacgccct gtacttcatc gtggccttcc
     5641 cgatcgtcta tcgcctcgac gaggctgccg agggcgagcg ctggagcctg gggcgcgtga
     5701 tcatcgaggc ctcctgcgtg ggcatgctga cactgctgct gatcgacctg gcaac
//
LOCUS       DAORUQ010000188         5730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_16804_length_5730_cov_6.573392, whole genome shotgun sequence.
ACCESSION   DAORUQ010000188 DAORUQ010000000
VERSION     DAORUQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16804_length_5730_cov_6.573392"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLW13_13435"
     CDS             complement(<1..670)
                     /locus_tag="PLW13_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY71431.1"
                     /translation="MTDILYEQRGTVAWITINRPEARNTLSPAAFVALADTWQEVRDN
                     RDIRVAVLTGAGDRDFCCGGDLGGLIPLWTGARKPENAVEERLLADPAIGDRMLLKGE
                     PLYKPVIAAINGRALGGGCELLAASDVRIAASNAQFGLPEPRSGIVPGGGSMVRLARQ
                     IPYAHAMHLLMTGEPIDAPTALAWGLISEVVAPHELRARAEALATRIAANAPLALAAI
                     KRTVI"
     gene            complement(691..1809)
                     /locus_tag="PLW13_13440"
     CDS             complement(691..1809)
                     /locus_tag="PLW13_13440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQY71432.1"
                     /translation="MNNALCRRLGIELPIFAFTHCRDVVVAVSRAGGIGVLGAAGFTP
                     EQLGQELDWISARLGDKPFGIDVIMPKKFESGEVPDLQSMIPAGHKAWVEDVLARHGV
                     APLPADSDAGNHHGISGEQIGWTHDLCRQLLDVAFGYPGVKVMVNALGTPPADVLAEC
                     RARGILVGALAGKVKHALAHKHGGVDFVIAQGHEAGGHTGEITTMVLVPQVVDAVAPL
                     PVLAAGGIATGRQVAAAMALGAAGVWCGSVWLTTPESEVAPLPKKKLLAAGSDATVRS
                     RVISGKPARMLRSGWTDAWESPDCPGFLPLPLQGMLTQEAHLRIARSDNEDLAFYPCG
                     QSVGLCNDERDCRTVIADMLNEYVDTVERLGRSLAGDE"
     gene            complement(1806..2531)
                     /locus_tag="PLW13_13445"
     CDS             complement(1806..2531)
                     /locus_tag="PLW13_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71433.1"
                     /translation="MSPALDGLRERAKAGRRQRILDAVLAILCEEGLPALSTARISAR
                     AGVSVATLYNLIGGVEQIVDRLVEQLFAGMEQAMLEPVPIDSPAPEFEGYVEATWRYL
                     AAREEPLRAAQRAVFQRALAIGASNPVMRVAARSIGRLAGAVRALQREGRLSADADAR
                     LLAEQMVSCQSMQLENWSVGLLSLEHYRLGTRYQFLMLLRAWATPASATALDTALRQR
                     QQQLKALDTRRHHRQPQTGEASS"
     gene            2760..4649
                     /locus_tag="PLW13_13450"
     CDS             2760..4649
                     /locus_tag="PLW13_13450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY71434.1"
                     /translation="MPEPAAPRDVAVKGMPLAFWLPLLTALLFVAVIAVGSWLSWQNG
                     QRAVADLVGQLQLRAGKQVDEHLRSYLDSPRIAVEIIAGALEEGEITPERPATIHALL
                     LRLARAYDGVSYLNIGLEDTRFFGLGRVDNVSRDLVIEEAGPPDLTVLRQYGIDANGA
                     RAGLRKELPFSDIREQAWYREPLTAGRTSWTSIYNWVDNPDVVVIGLGKPVRFGPEAT
                     RAVVGIDLFLANICDYLAALDLSPGARVFIVERDGTLVASSHGDTLRRGAGVAPQLQP
                     VAEAEDPLIRLAALHIQAGDGLAGIREPRLATFSAEDARYFLRVSPWRDSLGLDWLIA
                     VVVPEADFTATIRANLRITLVAGAALLVLALGLALLMARFISHPVRRIEVAAKAVAEG
                     DFDTPLPRTAYREFNRLSQAFDDMTRRLRAAFREVEVAHDELEQKIAQRTEELREAND
                     ELERLSRQDSLTGLANRRRFDPGLREEWLRARRSGEPLTLVMCDLDYFKAYNDRYGHA
                     RGDEVLVKVARVLQSELRRSSDLAARYGGEEYALILPNTTADDALAVLAQIRAGMASL
                     GLVHEGSPFGRLTMSFGIACHHAGSEFASPEELLVAADQALYRAKASGRDRVAVHGKL
                     RENVA"
     gene            4646..5308
                     /locus_tag="PLW13_13455"
     CDS             4646..5308
                     /locus_tag="PLW13_13455"
                     /inference="COORDINATES: protein motif:HMM:NF018777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HQY71435.1"
                     /translation="MKSLCLLTRRAGTTREAFRDYYETQHCRLGMKYFPFAKYLRNHV
                     VAASVDIDFDCVSEFYFDAGRGGADLMATPVGDILRADERKFMDQGLIRPAVAEESIL
                     AGPPRDVAAPGTQRQLLMLDPADGAADADFFAALRDWGLALGAAPGMLRVSLDRATPF
                     APGAGGFPWRAMLSLWLAPGAAPVPIPAPPATLTLGVALLADVCESPPELIRALYAPE
                     SN"
     gene            5329..5730
                     /locus_tag="PLW13_13460"
     CDS             5329..5730
                     /locus_tag="PLW13_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71436.1"
                     /translation="MSRKTFVQRIAALAATLVLAAPALGQETAEVELKAGYVDPASGV
                     KVEEIVVMPEQDMQAVRLAVPRSGEPIEEMVVTAQRRSGEPVLQLKPHEFVRDYDHNY
                     YGLVIYLGKNESLPLRLYMDAAQQRPGPITP"
BASE COUNT          825 a         2022 c         2011 g          872 t
ORIGIN      
        1 cgatcacggt gcgcttgatc gcggcgagcg cgagcggggc gttcgccgcg atgcgcgtcg
       61 cgagcgcctc ggcgcgcgcg cgcagctcgt gcggcgccac cacctcgctg atgagccccc
      121 aagcgagcgc cgtgggcgcg tcgatgggct cgccggtcat cagcaggtgc atcgcgtggg
      181 cgtaggggat ctggcgcgcc aggcgcacca tcgagccgcc cccgggcacg atgccggagc
      241 ggggctcggg caggccgaac tgcgcgttgc tggcggcgat gcgcacgtcg ctggccgcca
      301 gcagctcgca gccgccgccg agcgcgcggc cgttgatcgc ggcgatcacc ggcttgtaca
      361 gcggctcgcc cttgagcagc atcctgtcgc cgatcgccgg atcggccagc aggcgctcct
      421 cgacggcgtt ttcgggcttg cgcgcgccgg tccacaacgg gatcagcccg ccgagatcgc
      481 cgccgcagca gaagtcgcga tcgccggcgc cggtgagcac cgcgacgcgg atgtcgcggt
      541 tgtcgcgcac ttcctgccag gtgtcggcca gtgccacgaa ggccgccggg ctcagggtgt
      601 tccgcgcctc ggggcggttg atggtgatcc acgcgaccgt gccgcgctgt tcgtagagga
      661 tgtcggtcat ggtgtcgtct ccggggtgat tcattcgtcg ccggcgagcg agcggccgag
      721 ccgctccacc gtatcgacgt attcgttcag catgtcggcg atcaccgtgc ggcagtcgcg
      781 ctcgtcgttg cacaggccga cggactggcc gcagggatag aaggcgagat cctcgttgtc
      841 cgagcgcgcg atgcgcaggt gcgcttcctg cgtcagcatg ccctgcagcg gcagtggcag
      901 gaagcccggg cagtccggcg actcccatgc gtcggtccag cccgagcgca gcatgcgcgc
      961 gggcttgccc gaaatcaccc gcgaacgcac ggtggcatcg ctgcccgcgg ccagcagctt
     1021 cttcttcggc agcggcgcga cctcggattc cggcgtcgtc agccacaccg agccgcacca
     1081 cacgccggcc gcccccagcg ccatcgcggc ggcgacctgg cgcccggtgg cgatcccgcc
     1141 agcggcgagc acgggcagcg gcgcaaccgc gtccaccacc tgcggcacca gcaccatcgt
     1201 ggtgatctct ccggtgtggc cgccggcctc gtgcccctgc gcgatcacga agtccacgcc
     1261 gccgtgcttg tgcgccagcg catgcttcac cttgccagcg agcgcgccga ccagtatccc
     1321 gcgcgcgcgg cattcggcga gcacgtcggc gggcggcgtg ccgagcgcgt tcaccatcac
     1381 cttcacgccc gggtagccga aagccacgtc gagcagctgg cggcagaggt cgtgcgtcca
     1441 accgatctgc tcgccgctga taccgtgatg gttgccggca tcggaatccg ccggcagcgg
     1501 cgccacgccg tggcgcgcca gcacgtcctc gacccaggcc ttgtggccag cggggatcat
     1561 ggactgcagg tccggcacct cgcccgactc gaacttcttc ggcatgatca catcgatgcc
     1621 gaagggcttg tcgccgagcc tggcgctgat ccagtcgagc tcctgcccga gctgctccgg
     1681 cgtgaaaccc gcggcaccga gcacgccgat gccacccgcg cgggagacgg ccacgaccac
     1741 gtcgcgacag tgggtgaagg cgaagatcgg cagctcgatg ccaagacgtc ggcacagcgc
     1801 gttgttcatg atgaagcctc tcctgtttgc ggttggcggt ggtggcgcct ggtgtccagc
     1861 gccttcagtt gttgctggcg ttggcgcagc gccgtatcca gcgccgttgc gctcgccggt
     1921 gtggcccagg cgcgcagcag catcagaaac tggtagcgcg tgcccagccg gtagtgttcg
     1981 aggctgagca ggcccacgga ccagttctcc agctgcatcg actggcagga caccatctgc
     2041 tcggcgagca ggcgcgcgtc ggcatccgcg ctcagccggc cctcgcgctg cagcgcgcgc
     2101 acggccccgg caaggcgtcc gatgctgcgc gcggccacgc gcatcaccgg gttgctcgca
     2161 ccgatcgcca gcgcgcgctg gaaaaccgcc cgctgcgccg cgcgcagcgg ctcctcgcgc
     2221 gcggcgaggt agcgccaggt tgcttcgacg tacccctcga actccggcgc cggactgtcg
     2281 ataggtaccg gctcgagcat tgcctgctcc atgcccgcga agagctgctc gaccaggcgg
     2341 tcgacgatct gctccacccc tccgatcagg ttgtagagcg tggccacgct cacgccggcg
     2401 cgcgcgctga tgcgtgcggt cgagagcgcc ggcaacccct cctcgcagag tatcgccagc
     2461 accgcatcga ggatgcgctg gcgccggccg gccttggcgc gctcgcgcag tccgtccagg
     2521 gcggggctca tggttgccgg cgtcgttgtc atgacgggac cgaggatata gaaaaagtgg
     2581 aaacactttc taaatttttc ttcatcccgg gcgtgccccg gcgggcgagg ggtgatcgtg
     2641 actacctctg cggcgcggat gacgttagtc tggcgccctg tccgagcgcg agtgcagtgg
     2701 cacccgcaca gcatggagga gccagccgct tgcccgcttt ccgtcccctc gagtccgcag
     2761 tgcctgagcc ggccgcgccg cgcgatgtcg ccgtcaaggg catgccgctg gcgttctggc
     2821 tcccgctgct gaccgcgctg ttgttcgtcg cggtgatcgc ggtgggcagc tggctgtcgt
     2881 ggcagaacgg gcagcgcgcg gtggccgacc tcgtcgggca gttgcagttg cgcgccggca
     2941 agcaggtcga cgagcatctg cgcagttatc tcgattcgcc gcgcatcgcg gtggagatca
     3001 tcgccggcgc gctcgaggag ggcgagatca cacccgagcg gcctgccacc atccatgccc
     3061 tgctgctgcg cctggcgcgg gcctacgacg gtgtcagtta cctcaatatc gggctggagg
     3121 acacccggtt cttcgggttg ggccgggtgg acaacgtctc gcgcgacctg gtcatagagg
     3181 aggccggccc gcccgatctc acggtgctgc gccagtacgg catagacgcc aacggcgccc
     3241 gcgccggact gcgcaaggag ctgccgttca gcgatatccg ggagcaggcg tggtatcgcg
     3301 aaccgctcac tgcgggccgc acctcctgga ccagcatcta caactgggtc gacaatcccg
     3361 acgtggtcgt gatcgggctg ggcaagccgg tgcgcttcgg gccggaggcg acgcgggcgg
     3421 tggtgggcat cgacctgttc ctggccaata tctgcgatta cctcgcggcg ctggacctga
     3481 gccccggtgc gcgcgttttc atcgtcgagc gcgacgggac tctggtcgcc agctcgcacg
     3541 gcgacacgct gcgccgcggc gctggcgttg cgccgcagtt gcagccggta gccgaggccg
     3601 aagacccgct gatccggctc gccgcgctgc atatccaggc gggcgacggc cttgccggca
     3661 tccgcgagcc gcgcctcgcg acgttcagcg ccgaggacgc gcgctacttc ctgcgcgtct
     3721 cgccctggcg cgacagcctg gggctggact ggctgatcgc ggtggtggtg ccggaggcgg
     3781 atttcacggc gacgatcagg gccaacctgc gcatcacgct cgtcgcgggc gctgccctgc
     3841 tggtgctggc gctcggcctg gcgctgctga tggcgcgctt catctcgcat cccgtgcgcc
     3901 ggatcgaggt ggcggccaag gctgtcgccg agggcgattt cgacacgccg ctgccgcgca
     3961 cggcgtatcg cgaattcaac cggctctcgc aggccttcga cgacatgacc cggcgcctgc
     4021 gcgcggcgtt tcgcgaagtg gaggtggcgc acgacgaact ggagcagaag atcgcgcagc
     4081 gcaccgagga actgcgcgag gccaacgacg aactcgagcg actgagccgc caggacagcc
     4141 tgacgggcct ggcgaaccgc cgccgcttcg accccggtct gcgcgaggaa tggctgcgcg
     4201 cgcgccgcag cggcgaaccg ctgacgctgg tgatgtgcga cctcgactac ttcaaggcct
     4261 acaacgaccg ctacgggcat gcccgcggcg acgaggtgct ggtgaaggtg gcgcgcgtgc
     4321 tgcagtccga gctcaggcgc tccagcgatc tcgccgcgcg ctacggcggc gaggagtacg
     4381 cgctgatcct gcccaacacc acggccgacg acgcgctggc cgtgctcgcg cagatccgcg
     4441 ccggcatggc ttcgctcggg ctggtgcacg agggctcgcc gttcgggcgc ctcaccatga
     4501 gcttcggcat tgcctgccac cacgcgggca gcgaattcgc ctcgcccgag gagctgctgg
     4561 tcgcggccga ccaggcgctc taccgggcca aggcgagcgg tcgcgatcgc gtggccgtcc
     4621 acggcaaact cagggaaaac gttgcatgaa atcgctctgc ctgctcacgc gccgcgccgg
     4681 caccacgcgc gaggctttcc gcgactacta cgaaacgcag cactgccggc tcggcatgaa
     4741 gtacttcccg ttcgcgaagt acctgcgcaa ccacgtcgtc gcggcatccg tggacatcga
     4801 cttcgactgc gtgtcggagt tctacttcga cgcgggtcgc ggcggcgccg acctgatggc
     4861 cacgcccgtc ggcgacatcc tgcgcgccga cgagcgcaag ttcatggacc agggcctgat
     4921 ccgaccggcc gtggccgagg agagcatcct cgccgggccg ccgcgcgacg tggcggcgcc
     4981 cggcacgcaa cgccagctgc tgatgctgga cccggccgac ggcgcggcgg acgcggactt
     5041 tttcgccgcg ctgcgcgact ggggcctggc gctgggtgcc gcgcccggca tgctgcgcgt
     5101 gagcctggat cgcgccacgc ccttcgcgcc cggtgcgggc ggcttcccgt ggcgggcgat
     5161 gctgagcctg tggctggcgc cgggggcggc tcccgtgccc atcccggcgc cgccggcgac
     5221 gctcacgctc ggtgtcgcgc tgctcgccga cgtgtgcgag agcccgcccg aactcatccg
     5281 ggcgctgtac gcccccgaat cgaactgacc atccaaggag tagttgtcat gtcccgaaag
     5341 acattcgtgc agcgcatcgc cgcgctcgcc gccacgctcg tgctcgccgc ccccgcgctg
     5401 ggccaggaaa cggccgaggt cgaactgaag gcaggctacg tcgatccggc gagcggcgtg
     5461 aaggtcgagg agatcgtggt gatgcccgag caggacatgc aggccgtgcg cctggcggtg
     5521 ccgcgcagcg gcgagccgat cgaggagatg gtcgtgacgg cacaacggcg ctccggcgag
     5581 ccggtgctgc aactgaagcc gcacgagttc gtgcgcgact acgaccacaa ctactacgga
     5641 ctggtgatct atctcggcaa gaacgagagc ctgccgctgc gcctgtacat ggacgccgcg
     5701 cagcaacgcc cgggcccgat cacgccctga
//
LOCUS       DAORUQ010000189         5677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17053_length_5677_cov_5.052472, whole genome shotgun sequence.
ACCESSION   DAORUQ010000189 DAORUQ010000000
VERSION     DAORUQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17053_length_5677_cov_5.052472"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..705)
                     /locus_tag="PLW13_13465"
     CDS             complement(106..705)
                     /locus_tag="PLW13_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71437.1"
                     /translation="MRTALLLLLSFALAGCGPKLVTMKPLNEFPEPLVEPLPVSVGVY
                     FAPEFVSFTHKEKRPGPAGEEWTINLGTPQLQVFRAIFDATFTAARELEAPERGDGGI
                     QAIIVPSVTDFQFALPDDTRAKVFEIWVKYDLSIRDPQGEEIGHWAFTAYGKTPTAFL
                     KTDEEAIQAATVVALRDAGASMITGLQRDPRIREWLGVP"
     gene            complement(775..1770)
                     /locus_tag="PLW13_13470"
     CDS             complement(775..1770)
                     /locus_tag="PLW13_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71438.1"
                     /translation="MRKARRSAEEFSLSFLDVICCGFGAIILLLMITKIAEPLVLEES
                     TRDLRGQIAALQEELHRLRGESVVLNRDLTARREQLSDSLAAVARLEGDLSAVKGQYR
                     ASSTDADVNSRLVGQLESARQRLTDEMKRLLGAQGRRRNDAIGGIPVDSEYVIFIIDT
                     SGSMFNYAWPRAQREMINILDLYPQVKGMQVMSDEGEYMFPSYRGQWIPDTPARRRAI
                     VSRFATWNPYSNSSPVEGIVQAIRTFHQPGRRISLYVLGDEFTGSSIAQVIDVVDRIN
                     SESGGERLVRIHAVGFPTQFARPDNLQVTGIRFASLMRDLAWRNGGTFVGLNDYR"
     gene            complement(1767..2855)
                     /locus_tag="PLW13_13475"
     CDS             complement(1767..2855)
                     /locus_tag="PLW13_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71439.1"
                     /translation="MARKRRGFSIFSLSFLDIMSCGFGATILVFLVIDHASDVRSEEA
                     NRDLIGEVNFLEREVREGREGLAQLRNTIAMVDESAVEAKGRSREILAEIRRRREELS
                     TDDKDTLASSEHVNKLKADLKQLEEQNERLRAEGEKQQGTDVRRFTGDGDRQYLTGLK
                     LGGRRILVLLDRSASMLDNTLVNVIRMRNMPETVQRRAPKWRRAVATVDWLSTQLPRD
                     SEYQIYVFNTGAAPVLPDTAGKWLPVANREQLDKAIAALDAVTPAAGTSLLGAFQAAA
                     AMNPKPDNIYLITDGLPTQGSGTAGAKASGRDRLKYFNQALNSLPDGIPVNVILAPME
                     GDPMAASAFWQLAMATRGAFLAPASDWP"
     gene            complement(2845..4215)
                     /locus_tag="PLW13_13480"
     CDS             complement(2845..4215)
                     /locus_tag="PLW13_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71440.1"
                     /translation="MTLSVLELNDIALLLGNGTGTPTRSPGFATLTRDAVVTGAAALA
                     QARLNPRQTVSQFWLRLGTEPLHNARSHARHHADLAWAQLQQLATAAGSPAQLILAVP
                     GSFTREQLAVLLGVAQRASFRAVGLVDSALAAASTTHIPLSALHLDLQLHQCVLTRLV
                     RDGDMLVRERVHTVAGAGLLQLQERLAQLVTARFIHHSRFDPMHAAATEQQLYDRLPA
                     WMAALEDGEDVHAELRNGTASYHAKLGIGEVLEAVQPVYAQIVDAVQRERQGAALLCS
                     ARLAPLPRLGRLLPEAIRLPEDAAIRACLRHAALIRSEDAALRFVTRLPALVDVPGAG
                     LAQAPAPAAPARSGPAPAPAADTPSHLVAGARALRLAPGSLYLHEGADGWTLTRRLPP
                     DLAGTLSWDGAQWWLMPAAGHALALDGEPVRSATLLRAGQRLALPARRAELWVVAETP
                     VPDDGA"
     gene            complement(4218..5024)
                     /locus_tag="PLW13_13485"
     CDS             complement(4218..5024)
                     /locus_tag="PLW13_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY71441.1"
                     /translation="MKRILASEFLFQLFALLLSLIVVHTFYVAVVTPNAEAVARAAQE
                     RVAAGDTSARPPSAWVILKDYEQESCVVLMLWCIAIMGYKGAMLRREKQLLERPLVQV
                     PGGTSILPEDAREYLRPIQALPAEARRALLPRALITGLQRFAATHSIQDAAASIREVC
                     ETEGERLDSELSMVRYIGWAIPSIGFIGTVRGIGMALTQAHRAVEGDITGVTDALGVA
                     FNSTFVALVISILLMFLMHQLQLQQERLVLEAQAYCDDRLLGHLKVTREG"
     gene            complement(5054..5299)
                     /locus_tag="PLW13_13490"
     CDS             complement(5054..5299)
                     /locus_tag="PLW13_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71442.1"
                     /translation="MKRLALIAATLAALAALAALATAQEAPEDAPDAPQPATSPAAEP
                     APAAGEKAAPRGGAGDERFVPSEDISEDLSVSFPSDI"
     gene            complement(5296..>5677)
                     /locus_tag="PLW13_13495"
     CDS             complement(5296..>5677)
                     /locus_tag="PLW13_13495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQY71443.1"
                     /translation="FGWGQELPPSAKAGNFADQSGAALLGQVIAGYDDGFAVAAPRKR
                     FAPNRHGLFDLSGNAAEWMHDIYEALPAEARTDPTGASSGEFHVIRGASWRHGSVTEL
                     RLAFRDYGKEARADLGFRLARYAE"
BASE COUNT          787 a         1947 c         2027 g          916 t
ORIGIN      
        1 ctgcggcgcg cttcaccggc ggtgccggtc gccacggcac gtaggtccgg attcatccgg
       61 acatctgcgc aggaaatgcc ttcacgtgtc cggatgaatc cggacctacg gcacgcccag
      121 ccattcgcgg atgcgcgggt cgcgctgcag gccggtgatc atcgaggcgc cggcgtcgcg
      181 cagcgcgacc accgtggcgg cctgtatcgc ctcctcgtcg gtcttcagga acgcggtcgg
      241 tgtcttgccg taggcggtga aggcccagtg gccgatttcc tcgccctgcg ggtcgcggat
      301 actgaggtcg tatttcaccc agatctcgaa taccttcgcc cgggtgtcgt cgggcagcgc
      361 gaactggaaa tccgtcacgc tgggcacgat gatcgcctgg atcccgccgt cgccacgctc
      421 cggtgcctcc agttcgcggg cagcggtgaa cgttgcgtcg aaaatcgcgc ggaacacctg
      481 cagttgcggc gtgccgaggt tgatggtcca ttcctcgccg gcgggtccgg ggcgcttctc
      541 cttgtgcgtg aacgagacga attccggcgc aaagtacacg cccacgctga ccggcaacgg
      601 ttccaccagg ggctcgggaa actcgttcag gggcttcatc gtcacgagct tcgggccgca
      661 gccggcgagg gcgaaggaaa gcagcaggag cagggcagtg cgcatcgttg tccaccgtga
      721 gtgcgtccgg atcataggta caattgcggc accgatccag gcggcgccgg cgggctagcg
      781 atagtcattc aggccgacga aggtgccgcc gttgcgccac gcgaggtcgc gcatcagcga
      841 ggcgaagcgg atgccggtga cctgcaggtt gtcggggcgc gcgaactgcg tcgggaaacc
      901 cacggcgtgg atgcgcacca gccgctcgcc gcccgattcg ctgttgatgc ggtcgaccac
      961 gtcgatcacc tgcgcgatcg agctgccggt gaactcgtcg ccgagcacgt agaggctgat
     1021 cctgcgcccc ggttggtgga aggtgcggat cgcctgcacg atgccttcca ccgggctgct
     1081 gttgctgtac gggttccacg tcgcgaagcg gctcacgatc gcgcgccgcc gcgccggcgt
     1141 gtcggggatc cactgtccgc ggtagctcgg gaacatgtac tcgccctcgt cgctcatcac
     1201 ctgcatgcct ttgacctgcg ggtagagatc gaggatgttg atcatctcgc gctgcgcgcg
     1261 cggccaggcg taattgaaca tgctgcccga ggtgtcgatg atgaagatca cgtactcgct
     1321 gtcgaccggg atgccgccga tcgcgtcgtt gcgccgccgg ccctgcgcgc cgagcaggcg
     1381 tttcatctcg tcggtcaggc gctgcctggc gctctccagc tgacccacca ggcggctgtt
     1441 cacgtcggcg tccgtgctgc tcgcgcggta ctgtcccttg accgcggaga gatcgccctc
     1501 gagccgtgcc accgccgcca gcgaatccga cagctgttcg cgccgcgccg tgaggtcgcg
     1561 gttcagcacc acggattcgc cgcgcagccg gtgcagttcc tcctgcagtg cggcgatctg
     1621 ccccctgagg tcgcgcgtgc tttcctcgag caccagcggc tcggcgatct tcgtgatcat
     1681 cagcaagagg atgatcgcgc cgaagccgca gcagatcacg tccaggaagg acaggctgaa
     1741 ttcctcggcg ctgcgccgcg ccttcctcat ggccagtcgc ttgccggtgc gaggaaggcg
     1801 ccgcgcgtgg ccatcgcgag ctgccagaac gccgaggccg ccatcgggtc gccttccatc
     1861 ggcgcgagga tcacgttgac cgggatgccg tcgggcaggc tgttcagcgc ctggttgaag
     1921 tatttcagcc ggtcgcggcc actcgccttg gcgcctgctg tgccgctgcc ctgcgtgggc
     1981 aggccgtcgg tgatcaggta gatgttgtcg ggcttggggt tcatggcggc ggcggcctgg
     2041 aaggcaccca gcaggctggt gcccgcggcg ggcgttaccg cgtcgagagc cgcgatcgcc
     2101 ttgtcgagct gttcgcggtt cgccaccggc agccacttgc cggcggtgtc gggcagcacc
     2161 ggcgcggccc cggtattgaa cacgtagatc tggtactcgc tgtcgcgcgg cagctgtgtg
     2221 ctcagccagt cgacggtcgc taccgcgcgg cgccacttgg gcgcgcgccg ctgcacggtt
     2281 tcgggcatgt tgcgcatgcg gatcacgttg accagggtgt tgtcgagcat gctcgccgag
     2341 cggtccagca gcaccaggat gcgccgcccg ccgagcttga ggccggtcag gtactggcgg
     2401 tcgccgtcgc cggtgaagcg gcgcacatcc gtgccctgct gcttctcgcc ttcggcgcgc
     2461 agccgctcgt tctgttcctc cagctgcttc aggtccgcct tcagcttgtt cacgtgctcg
     2521 ctgctcgcga gcgtgtcctt gtcatcggtg gagagctcct cccggcggcg ccggatctcg
     2581 gccaggattt cgcgcgatcg gcctttggcc tcgacggccg actcgtcgac catcgcgatg
     2641 gtgttgcgca gttgcgccag cccctcgcgt ccctcgcgca cctcgcgctc gaggaagttc
     2701 acctcgccga tcaggtcgcg gttggcttcc tccgagcgca cgtcgctcgc gtggtcgatc
     2761 accaggaaca ccaggatcgt cgcgccgaat ccgcaggaca tgatgtccag gaaggacagg
     2821 ctgaagatcg agaagccgcg gcgtttacgc gccatcgtcc gggaccggcg tttcggcgac
     2881 cacccacagc tcggcgcggc gcgccggcag cgcgaggcgc tggccggcgc gcagcagcgt
     2941 cgccgatcgc accggctcgc cgtcgagcgc gagcgcgtgg ccggccgcgg gcatcagcca
     3001 ccattgcgcg ccgtcccagc tcagcgtgcc ggcgagatcc ggcggcagcc ggcgcgtcag
     3061 cgtccagcca tccgcccctt cgtgcaggta caggctgccg ggcgcgaggc gcagcgcgcg
     3121 cgcgccggcg accaggtggc tgggtgtgtc tgcagcgggc gcgggcgccg gcccgctgcg
     3181 ggcgggtgcg gcgggcgcag gcgcctgggc gagcccggcg cccggcacgt cgacgagggc
     3241 cggcagccgg gtgacaaagc gcagtgccgc gtcctcgctg cggatcagcg cggcgtggcg
     3301 caggcaggcg cggatggccg cgtcctcggg caggcggatc gcctccggca gcagccgccc
     3361 gaggcgcggc agcggcgcga gccgcgcgct gcacaggagc gcggcgccct ggcgctcgcg
     3421 ctgcaccgcg tccacgatct gcgcgtacac cggttgcacc gcctccagta cctcgccgat
     3481 gccgagcttg gcgtggtagc tcgccgtgcc gttgcgcagc tcggcgtgga catcctcgcc
     3541 gtcctccagc gcggccatcc acgccggcag gcggtcgtag agttgctgct cggtagccgc
     3601 ggcgtgcatc gggtcgaagc ggctgtggtg gatgaagcgc gccgttacca gctgcgccag
     3661 gcgctcctgc aattgcagca gcccggcgcc cgccacggtg tgcacgcgct cgcgcacgag
     3721 catgtcgcca tcgcgcacca ggcgcgtgag cacgcactgg tgcaactgca ggtccaggtg
     3781 cagcgcgctg agcgggatgt gcgtggtgct tgccgccgcg agtgcgctgt cgaccaggcc
     3841 gacggcacgg aacgaggcgc gctgcgcgac cccgagcagc accgcgagct gttcgcgcgt
     3901 gaagctgccc ggcaccgcga ggatcagttg cgccgggctg cccgccgcgg tggccagctg
     3961 ctgcagctgc gcccaggcga ggtcggcatg gtggcgtgcg tggctgcgcg cgttgtgcag
     4021 cggttcggtg cccagacgca gccagaactg gctgacggtc tggcgcgggt tgagccgtgc
     4081 ctgcgccagc gccgccgcgc cggtcaccac ggcgtcgcgc gtcagggtcg cgaaaccggg
     4141 actgcgggtc ggcgtgcccg tgccgttgcc cagcagcaac gcgatatcgt tcagttccag
     4201 taccgacagc gtcacgctca gccctcccgc gtcaccttga ggtggccgag caggcgatcg
     4261 tcgcaatagg cctgtgcctc cagtaccaga cgttcctgct gcaactgcag ctggtgcatc
     4321 aggaacatca gcaggatgct gatcaccagc gcgacgaaag tcgagttgaa cgccacgccg
     4381 agcgcgtcgg tgacgccggt gatgtcgccc tcgacggcac ggtgcgcctg cgtcagcgcc
     4441 atgccgatac cgcgcacggt gccgatgaag ccgatcgagg gaatggccca gccgatgtag
     4501 cggaccatcg agagctcgga atcgaggcgc tcaccctccg tctcgcacac ctcgcggatc
     4561 gaggcggcgg catcctggat gctgtgcgtc gcggcgaagc gctgcagccc cgtgatcagt
     4621 gcgcgcggca gcagcgcgcg gcgcgcctcg gcgggcagcg cctggatcgg gcgcaggtat
     4681 tcgcgcgcgt cctcgggcag gatgctggtg ccgcccggca cctgcaccag cggccgttcc
     4741 agcagctgct tctcgcgtcg cagcatcgcg cccttgtagc ccatgatcgc gatgcaccac
     4801 aacatcagca ccacgcagct ctcctgctcg tagtccttca ggatcaccca tgccgagggc
     4861 ggacgcgccg aggtgtcgcc ggcggcgacg cgctcctgcg cggccctcgc gacggcctcg
     4921 gcattcggcg tcaccacggc gacgtagaag gtgtgcacca cgatcagcga cagcagcagg
     4981 gcgaagagct ggaacaggaa ctcggaggcg aggatgcgtt tcatggggtc cttgtcgtgg
     5041 cgcggcgcct ggttcagatg tccgatggga atgacacgga gagatcctcc gagatgtcct
     5101 cgctgggcac gaagcgctcg tcgccggcgc cgccgcgcgg ggcggccttc tcgccggcgg
     5161 cgggcgcggg ctccgcggcc ggcgatgtcg cgggctgggg tgcgtccggc gcgtcctccg
     5221 gcgcttcctg cgccgtggcg agcgcggcga gcgcggcgag agcggcgagc gtggcggcga
     5281 tgagcgcgag ccgcttcatt cggcgtacct cgcgagacgg aaacccaggt cggcgcgcgc
     5341 ttccttgccg tagtcgcgaa aggcgaggcg caactcggtg acgctgccgt ggcgccagct
     5401 cgcgccgcgg atcacgtgga attcgccgct gctggcgccc gtgggatcgg tgcgagcctc
     5461 ggcgggcagc gcttcgtaga tgtcatgcat ccattctgcc gcattgccgc tcaggtcgaa
     5521 aaggccgtga cggttgggcg cgaagcgctt gcgcggtgcg gcgaccgcga agccgtcgtc
     5581 gtagcccgcg atcacctgcc cgagcagcgc ggcgccgctc tggtcggcga aattcccggc
     5641 cttcgccgac ggcggcagtt cctggcccca gccgaag
//
LOCUS       DAORUQ010000190         5639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17219_length_5639_cov_5.188574, whole genome shotgun sequence.
ACCESSION   DAORUQ010000190 DAORUQ010000000
VERSION     DAORUQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17219_length_5639_cov_5.188574"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PLW13_13500"
     CDS             complement(<1..1026)
                     /locus_tag="PLW13_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY71444.1"
                     /translation="MRRLPLQGRTLALLAFIVPLLALFVFVGLRSGPLAPVAVTLATV
                     QSRALAPALFGIGTVEARYTYRIGPVVAGRVERLDVDVGDRVRAGQVLGAMDPVDLDA
                     RVRALDSASKRAGATLREAEARQAYAQAQARRYEKLLLARSISEETVTTKRQELQIAD
                     AVLAAAREEVDRARAEREALIAQRSDMRLVAPVDGVVVLRDAEPGSTVVAGQAVVELI
                     DPASLWINVRFDQLSAAGLATALPARIVLRSRDGEALAGRVFRIEPKADAVTEETLAK
                     VVFENVPSPLPPVGELVEVTVGLPGLPAAPVIPNAAIRREGGKAGAWRMGDGELRFVP
                     LVLGATDL"
     gene            complement(1023..1631)
                     /locus_tag="PLW13_13505"
     CDS             complement(1023..1631)
                     /locus_tag="PLW13_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71445.1"
                     /translation="MENRPKHLPAEQRRAVTVEAVVALAARQNPSEITTAAIARHMKL
                     TQGALFRHFPSKEAIWEAVMEWVAERLLARIDKAAEGIESPVAAMEAMFMSHVEFVIE
                     HPGVPRMMFGELQRTDASAAKRMVQTLMMRYRERLQRLIARGMSGGELSIALDEEAAA
                     TLFIGTIQGLVMQSLMAGDVARMRRAAPRVFAIYRRGIGSAS"
     gene            complement(1924..3951)
                     /locus_tag="PLW13_13510"
     CDS             complement(1924..3951)
                     /locus_tag="PLW13_13510"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HQY71446.1"
                     /translation="MSTPLPRRSLAWLLFALVLAIGPHALRLPPWVLVVMGVAIAWRY
                     RVHQGAWSFPTRPVKLLLVVMSFLAVGLHWRALSGLEPAVALLAIAGGLKAMEMRTTR
                     DFLVVVFVAYFLVASQLLFEQEIPYALYAVVATAGVTAAMVARHQGDPAPGFPHPLVL
                     TGKLLAQAVPVMLLLFVVFPRIAPLWAIPQNMQSARTGPSDSMSPGDVARLSGSNALA
                     LRATFEGAIPAQRELYWRGLVFSEFDGRQWRQGAMARMEAELAQQGAPRPALRQLTHV
                     PSGEETSYEVILEPSNQPWAYVIGFPVSFDANLRSGSDYRLMSSRPLTQRLRYRVRAD
                     LGAQLEPELGILRRRTELQLPEGFNPRALAQAKAWRAEAPSDAAYIERVLRWFTEEEF
                     VYTMSPPLLGRDTVDEFLFGERRGFCEHYASAFAVLLRAAGIPARVVVGYQGGERNPY
                     QAYLLVHQFDAHAWTEAWLDGRGWVRFDPTAAVAPQRIESGAGEALGAEFLADSPLAI
                     ERYRNVRLLGWMRMRWDMMTYHWARLVLNYDAERQGALLSNLLGRISPARVVAVMLGC
                     GALVLLLVAASLFGVKPRPRRDAATAAYLRICERLAASGLERRRGEGPFDYARRVTAV
                     RPDLGAELQALTADYVALAFAQGAAGDRALPMQRLRQAVRRFRPRRAPIRA"
     gene            complement(3948..4883)
                     /locus_tag="PLW13_13515"
     CDS             complement(3948..4883)
                     /locus_tag="PLW13_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQY71447.1"
                     /translation="MSRRIPPARELVLTQRNIFIFPTGSGFAFLALLLALLITGINYE
                     SNLVFALTFLLGGLFIVGVLHTYANLAQLRLCAGASPPVFAGQQASFAVQLRDEGRRA
                     HDGITLSWPDGDEIGARVAPLGEDHVQLFTRAARRGWLHPGRLRLESRYPLGLLRAWS
                     WLDLDMRCLVYPRPAPAGPLPLDAGRGEEGSATHDPGAEDFLGFRNYAPGDPLRHVAW
                     KTLAKGQPLQTREYVAFADRRVWLTWAQTADWGATEQRLALLCRWVLELHALGADFGL
                     DIPGTRLEPAAGEAQRDRALAALALFGMAGEGARA"
     gene            complement(4946..>5639)
                     /locus_tag="PLW13_13520"
     CDS             complement(4946..>5639)
                     /locus_tag="PLW13_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY71448.1"
                     /translation="LGVSVYDQRAQAFNFHPGPVFTEVLLADEINRSTPKTQSALLEA
                     MAERQVTIEGETRALPPDFFVIATQNPVTQAGTFPLPESQLDRFLMRIEIGYPDRAAE
                     RAMLAGGAGTPPIEALESALSREQLRRIQHEAQQVGTADMLLDYVQRLIEYTRSAPEF
                     AHGLSPRGALALVNSARTWAYMEGRVHVVPEDVQAVLPAVVAHRLRSTTDFTGDGRAL
                     VQRLLADVDIFD"
BASE COUNT          893 a         2066 c         1918 g          762 t
ORIGIN      
        1 gagatcggtg gcgccgagca ccagcggcac gaagcgcaat tcgccatccc ccatgcgcca
       61 cgcgcccgcc ttcccgccct cgcgccggat ggctgcgttg gggatcacgg gtgcggcggg
      121 tagcccggga aggcccaccg tcacctcgac cagttcgccc acgggcggca gggggctggg
      181 tacgttctcg aacaccactt tcgccagcgt ttcctcggtg accgcgtcgg ccttgggctc
      241 gatgcggaac acgcgacccg cgagcgcctc accgtcgcgc gaacgcagca cgatgcgcgc
      301 gggcaatgcc gtggccagtc ccgccgcgct gagctggtcg aagcgcacgt tgatccacaa
      361 gctcgcggga tctatcagtt ccaccaccgc ctgccccgcc accacggtcg agccgggctc
      421 ggcatcgcgc aacacgacca cgccatcgac cggcgcaaca agacgcatat cgctgcgctg
      481 cgcgatcagt gcctcgcgct cggcgcgcgc gcgatcgact tcttcgcggg cggccgccag
      541 cacggcatcc gcgatctgca gctcctggcg cttcgtggtg acggtctcct cgctgatcga
      601 gcgcgccagc agcagttttt cgtagcggcg cgcctgcgct tgcgcatacg cctgccgggc
      661 ctctgcctcg cgcagcgtcg cgccggcccg cttggacgcg gaatccagcg cgcgcacccg
      721 ggcatccaga tccacagggt ccatcgcacc gagtacctgc cccgcgcgga cccggtcgcc
      781 cacgtccacg tcgagccgtt cgacgcgtcc ggcaacaaca ggtccgatcc ggtacgtgta
      841 acgggcctcc acggtgccga tgccgaacag cgccggcgcg agggcgcgcg attgcaccgt
      901 ggccagcgtc accgcgacgg gcgccagcgg tcccgagcgc aacccgacga acacgaagag
      961 tgcgagcagc ggcacgatga acgcgagcag cgccagcgtg cggccctgca acggcaggcg
     1021 tctcatgacg cgctcccgat accgcgacga tagatggcaa aaacgcgcgg cgcggcgcga
     1081 cgcatgcgcg ccacatcgcc tgccatgagt gactgcatca ccaggccctg gatggtaccg
     1141 atgaacagcg tcgccgcggc ctcctcatcg agcgcgatcg acaactcgcc gccggacatg
     1201 cccctggcga tcaggcgctg aaggcgctcg cggtatcgca tcatgagcgt ctgcaccatg
     1261 cgcttggccg ctgacgcgtc cgtacgctgc aactcgccga acatcatccg cggaacgccg
     1321 ggatgctcga tcacgaattc cacgtggctc atgaacatcg cttccatcgc cgccacgggc
     1381 gattcgatcc cctcggctgc cttgtcgatt cgagccagca ggcgctcggc tacccattcc
     1441 atcaccgcct cccagatggc ctccttgctc gggaaatgtc gaaacagcgc gccctgcgtg
     1501 agcttcatat gcctggcgat ggcggcggtg gtgatttcgc tggggttctg cctggcggcc
     1561 agcgcgacta cggcctctac tgtcacggct cgacgttgct ctgcgggcag gtgcttggga
     1621 cgattttcca tgatcactgc ataaaagata gtaattgatt actatcgtgc gccgagaaag
     1681 ccggtattac aagcagaaga agcagcgacg gcgtcgcggc gcgcaggcag actgacgggt
     1741 gcgccggtac gaatcgtacc cattgcaagt ggccaggatc gtgcacgaat gcttgttctg
     1801 acgctgtgcc tgtggacgct gctcctggag gaagacccgc gcccgtgtgc accatcacga
     1861 tcctcggcct ggcctgctgg atcgcgctgt gcgcgggtga atggtcgcag gcagggcccg
     1921 ctcctacgct cggattggcg cacggcgcgg gcggaagcgg cgcaccgcct ggcgcagccg
     1981 ctgcatcggt agcgcgcgat cccccgccgc tccctgcgcg aacgccagcg ccacatagtc
     2041 agccgtcaac gcctgcagtt ccgcgccaag atcagggcgc acggccgtca cgcgccgcgc
     2101 gtaatcgaaa gggccctcgc cgcggcggcg ttccagcccg gacgcggcca ggcgctcgca
     2161 gatccgcagg tacgcggccg tcgccgcatc gcgacgcggc cgtggcttca cgccgaacag
     2221 gctcgccgcg accagcagca gcacgagcgc gccgcaaccg agcatcaccg cgaccacgcg
     2281 cgccgggctg atccggccca gcaggttcga gagcagcgcg ccctggcgct cggcgtcgta
     2341 gttcagcacc agcctcgccc agtggtaggt catcatgtcc cagcgcatgc gcatccagcc
     2401 cagcagtcgc acgttgcggt agcgctcgat cgcgagcggc gaatcggcga gaaattccgc
     2461 acccagcgcc tcgcccgctc ccgactcgat gcgctgcggc gccactgcgg cggtgggatc
     2521 gaagcgcacc cagccgcgac cgtccagcca cgcctcggtc caggcgtggg cgtcgaactg
     2581 gtgcaccagc aggtatgcct ggtaggggtt gcgctcgccg ccctggtagc ccaccaccac
     2641 gcgcgccggg atgccagccg cgcgcagcag caccgcgaac gcgctggcgt agtgctcgca
     2701 gaagccgcgc cgctcgccga acaggaactc atcgaccgtg tcacgcccga gcagcggcgg
     2761 cgacatggtg tagacgaact cctcctcggt gaaccagcgc agcacgcgct cgatgtaggc
     2821 cgcatccgac ggtgcctcgg cgcgccaggc cttcgcctgc gcgagcgcgc gcgggttgaa
     2881 gccctcgggc agttgcagct cggtgcgccg gcgcaggatg ccgagctcgg gctccagctg
     2941 cgcgccgaga tccgcgcgca cgcggtagcg cagccgttgc gtgagcgggc gcgagctcat
     3001 caggcggtag tcgctgccgc tgcgcaggtt cgcatcgaac gagaccggaa agccgatcac
     3061 gtaggcccat ggctggttgc tgggctcgag gatcacttcg tagctggtct cttcgccgct
     3121 cggcacgtgc gtgagctggc gcagcgccgg gcgcggcgcg ccctgctgcg ccagctccgc
     3181 ctccatgcgc gccatcgcgc cctggcgcca ttgccggccg tcgaactccg agaacaccag
     3241 cccgcgccag tacagttcgc gctgcgccgg gatcgcgccc tcgaaggtgg cgcgcaacgc
     3301 cagcgcgttc gagccgctca ggcgcgccac gtccccgggc gacatgctgt cgctgggccc
     3361 ggtgcgcgcg ctctgcatgt tctgcggaat cgcccacagc ggcgcgatgc gcgggaacac
     3421 cacgaacagc agcagcatca ccggcacggc ctgcgcgagc agcttgcccg tcagcacgag
     3481 cggatgcggg aatcccggcg cgggatcgcc ctggtggcgc gcgaccatgg ccgcggtgac
     3541 gcccgcggtc gccaccaccg catacagcgc gtagggaatc tcctgctcga acagcagctg
     3601 gctcgcgacc aggaaatacg cgacgaaaac caccacgagg aaatcgcgcg tcgtgcgcat
     3661 ctccatcgcc ttcagcccgc cggcgatcgc cagcagcgcc accgcgggct ccaggccgct
     3721 cagcgcgcgc cagtgcagcc ccacggcaag gaacgacatc accaccagca gcagctttac
     3781 cgggcgggtc ggaaaagacc acgcgccctg gtgcacgcga tagcgccacg cgatcgcgac
     3841 acccatgacc accagcaccc acggcggcag ccgtagcgcg tgcggcccga tcgcgagcac
     3901 cagcgcgaac agcagccacg cgaggctgcg ccgcggcaag ggcgtgctca tgcgcgcgcc
     3961 ccctcgcccg ccatgccgaa cagcgcgagc gctgcgagcg cgcggtcgcg ctgcgcctcg
     4021 cccgccgccg gctcgagccg cgtgcccggg atgtcgagcc cgaagtcggc acccagcgcg
     4081 tgcagctcca gcacccagcg gcacagcagc gccaggcgct gctccgtggc gccccagtcc
     4141 gcggtctgcg cccaggtgag ccacacgcgg cgatccgcga aggccacgta ctcgcgcgtc
     4201 tgcaacggct ggcccttggc gagcgtcttc cacgccacgt ggcgcaaggg gtcgccgggt
     4261 gcgtagttgc gaaagcccaa gaaatcctcc gcgcccggat cgtgcgtggc gctgccttcc
     4321 tcgccgcgcc ccgcatcgag cggcagcggg cccgcgggcg ccgggcgcgg gtacaccagg
     4381 cagcgcatat cgagatccag ccacgaccac gcgcgcagca gccccagcgg atagcggctc
     4441 tccaggcgca ggcgccccgg gtgcagccag ccgcggcgcg cggcgcgcgt gaagagctgc
     4501 acgtggtcct cgcccagcgg cgcgacgcgc gccccgatct cgtcgccgtc gggccacgac
     4561 agcgtgatgc cgtcgtgcgc gcgcctgccc tcgtcgcgca gctgcaccgc gaagctcgcc
     4621 tgctggcccg cgaataccgg gggagacgcg cccgcgcaca ggcgcagctg cgcgaggttg
     4681 gcgtaggtgt gcagcacgcc gacgatgaac aacccgccca gcaggaaggt gagcgcgaac
     4741 accaggttgc tctcgtaatt gatcccggtg atcagcagcg ccagcagcag cgctaggaaa
     4801 gcgaagccgc tgccggtggg gaaaatgaag atgttgcgct gcgtcagcac gagctcgcgc
     4861 gccggcggga tgcgccggct cacccacgcg atccagcggg tgcgtacccg ccggcgcagg
     4921 gccgcggcac gcgccggcgc gcgcatcagt cgaagatgtc cacgtcggcc agcaggcgct
     4981 gcaccagcgc gcgcccgtcc ccggtgaagt ccgtggtgct gcgcaggcgg tgcgccacca
     5041 cggcgggcag cacggcctgc acgtcctcgg gcaccacgtg cacgcgtcct tccatgtagg
     5101 cccaggtgcg cgcgctgttg accagcgcca gcgcgccgcg cggcgacagg ccgtgggcaa
     5161 actcgggggc gctgcgcgtg tactcgatca gccgctgcac gtagtcgagc agcatgtcgg
     5221 ccgtgcccac ctgctgcgcc tcgtgctgga tgcggcgcag ttgctcgcgc gagagcgcgg
     5281 actcgagcgc ctcgatcggc ggcgtgccgg cgccaccggc gagcatcgcg cgctcggcgg
     5341 cgcggtcggg gtagccgatc tcgatgcgca tcaggaagcg gtcgagctgg gattcgggca
     5401 gcgggaaggt gccggcctgc gtgaccgggt tctgggtcgc gatgacgaag aagtccggcg
     5461 gcagcgcgcg cgtctcgccc tcgatcgtca cctggcgctc ggccatggcc tccagcagcg
     5521 cgctctgggt ctttggggtg ctgcggttga tctcgtcggc cagcagcacc tcggtgaaca
     5581 cgggaccggg atggaagttg aaggcctgcg cgcgctggtc gtagaccgag acgccgagg
//
LOCUS       DAORUQ010000191         5628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17267_length_5628_cov_5.680962, whole genome shotgun sequence.
ACCESSION   DAORUQ010000191 DAORUQ010000000
VERSION     DAORUQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17267_length_5628_cov_5.680962"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1344
                     /locus_tag="PLW13_13525"
     CDS             121..1344
                     /locus_tag="PLW13_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71449.1"
                     /translation="MNQCPMHTLLDFERFRHGTPRELISRLREEHRILWETDDYSGGH
                     WLLFHQDDIDHVLKTPELFTNNYSPILDDFPAEILAEQQQSMTFMDPPEHRKYRSLVD
                     YAFRLKQLEARKPLMRRIAQETVDRVIDRGECEFVNEIAMQMPMRVMFALLGVRDQDF
                     GYVVDLVNTLALADDPEFAENRALGFQASMELIEFGTRLAADHRQNPRDSMTMDVINT
                     AIDGRALGDREFGRFFNNLVVGGLETTRNTLALGLHQLILHPEQYRLLEQDPGLVAGA
                     VEEFLRHQNTVVYLRRTATRDMEYLGQQIGKGDKLVCILGAPNRDPALFPDPDAFDIT
                     RPVNNTRKNYRTFGQGPHYCVGVHQARLNLEVMLEEIVRRMTDFRLLAAPRHARSIFM
                     EGYKEMRVGFARRAA"
     gene            complement(1368..1787)
                     /locus_tag="PLW13_13530"
     CDS             complement(1368..1787)
                     /locus_tag="PLW13_13530"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HQY71450.1"
                     /translation="MSAIGEATLLAYLRCHYRVLGPPPYTLRIGERSAALAALFERSG
                     CDCAAFVTAWNPRGERHEAAANRAAQAALLRDIGARGYACIPGVGEDPDGQWAGEDSV
                     FVPAMGLAEAMLIAGSSGQNALVWAGPDARPQLILLR"
     gene            complement(1864..2808)
                     /gene="meaB"
                     /locus_tag="PLW13_13535"
     CDS             complement(1864..2808)
                     /gene="meaB"
                     /locus_tag="PLW13_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQY71451.1"
                     /translation="MDVVAGVLAGQVAAGARLIRWLEDDDPRGRAALGQVYPHTGRAH
                     LVGVTGPGGVGKSTLVDMLITEQRRRGRRVGVVAVDPSSPYTGGAILGDRVRMQRHAA
                     DEGVFIRSMGTRGQLGGLARASYDAMLVLDAMGYEVIFVETVGVGQDEIEIVALAHST
                     IIVAAPGLGDEIQAIKAGLMEAGDLFVLNKADRDGAMQTLRQLEMSLHLREQAVAADA
                     WKPPVLQAVAARGEGGAQIVDVLDRHAGFLRASGAFAAQRARRAHEVFVKLLRDAAAK
                     KILAAALARPDAAAVLADVAACRLDPHAAADLLLERIL"
     gene            complement(2811..3230)
                     /locus_tag="PLW13_13540"
     CDS             complement(2811..3230)
                     /locus_tag="PLW13_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQY71452.1"
                     /translation="MAQPPIRVLLAKLGLDTHTVGVTVIAHALRDAGMEVIFTGLKQT
                     PEMVANTAIQEDVDVVGVSSLSAGHMRNIPLLAELLRQKGGGDKLLIVGGVIPEEDQA
                     VLRAAGVARIFTMGADTGEIVAYLNEWRAARDAAGAS"
     gene            complement(3233..4996)
                     /locus_tag="PLW13_13545"
     CDS             complement(3233..4996)
                     /locus_tag="PLW13_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQY71453.1"
                     /translation="MGKVQEIEQATTAEPDAGAVQSEAARLEILRRQEARWQAGTRGP
                     VVAAQGERKESFRTQTMDWEIKALYTPLDLADSGFDYATDAGFPGEYPFTRGVDANGN
                     RSRLWTMAQVTGFGKGMAWAKRARYMLDQGLQGLILEYDLPTTNGYDSDNPLVAGEVG
                     RAGMALDSLEDLEEAFDLPFDKLKYLMSVCNAPQPVNLAMIIAALEKKGVDPGQLALH
                     IVNGILIEYTCVGRYIYPPGHGLRIATDCFEYIVRHHPHWQPISIIAAQLQPARANVV
                     QELAFSVTIAMAYIDAMLARGFDIDTVAPYFHFVINVDMDFFEGICKLRAFRKVWARL
                     MKERYGATRPEAMKLRVMTSPSTSSLTLQQPLNNIGRLAIMATACALAGAGETMTTPL
                     HDEAHALPAEDAIRVGAAIQHLVAHETGVADTIDPLAGSYYVESQTRRIEDESFAEIE
                     RIMNMGGALKAIEQGYFQRELGNEQYRRQREMEQGRRKLVGVNHLVVPEAKREMEIWR
                     LEDEMESQQVQKLQALRARRDNAAVQLALEKVRDAARTGTNLVEPCLAAVKLYATHGE
                     LCDAMRDVFGVHHADSQTAGV"
     gene            complement(5101..>5628)
                     /locus_tag="PLW13_13550"
     CDS             complement(5101..>5628)
                     /locus_tag="PLW13_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71454.1"
                     /translation="AEAVTKGIELDGAWRLTDTLTLGGSIAWLNAEYEDFENAPCGPF
                     LRAADPAGCVDGQDLGGETLQRAPDYSGTVYLEFTHAVGGGWDMDAYTGYTFRDESTT
                     VISNEFGADSLELLDARLSFSNADNGWMVALKGNNLTDERKLILSQDNDLLQGGQFGA
                     INMPRTYALQVRKNF"
BASE COUNT          878 a         2006 c         1845 g          899 t
ORIGIN      
        1 ctcgagccgg gcccacgatg acggcgcttg cgattcgagg cgcgcggacc gtagcatcgg
       61 cgccacgatg caatccgcag cgcatacggg cgcaaataca ctgcagggag acgcggcaca
      121 atgaatcaat gtccgatgca cacgctgctg gacttcgagc gctttcgcca cggcacgccg
      181 cgcgagctga tcagccggct gcgcgaggag caccggatcc tgtgggagac cgatgactac
      241 tccggcgggc actggctgct gttccaccag gacgacatcg accacgtgct gaagacgcct
      301 gagctgttca cgaacaacta cagcccgatc ctcgacgact tccctgcgga gatcctcgcc
      361 gagcagcaac agagcatgac cttcatggat ccgcccgagc accgcaagta tcgctcgctg
      421 gtggattacg cgttccgcct gaagcagctc gaggctcgca agccgctgat gcgccggatt
      481 gcgcaggaaa ccgtcgaccg cgtgatcgat cgtggcgagt gcgagttcgt gaacgagatc
      541 gcgatgcaga tgccgatgcg cgtgatgttc gcgctgctcg gcgtacgcga ccaggacttc
      601 ggctacgtcg tggacctggt gaacacgctt gcgctcgccg acgacccgga gttcgccgag
      661 aaccgcgcgc tcggcttcca ggcctcgatg gagctgatcg aattcggcac gcgcctcgcc
      721 gccgatcacc gccagaaccc gcgcgacagc atgacgatgg acgtgatcaa caccgcgatc
      781 gatggtcgcg cgctgggcga ccgcgagttc ggccgcttct tcaacaacct ggtcgtcggc
      841 ggcctcgaga ccacccgcaa cacgctcgcg ctcggcctgc accagctgat cctgcacccc
      901 gagcagtacc gcctgctcga acaggacccg gggctggtag ccggggcggt ggaggagttc
      961 ctgcgccacc agaacaccgt ggtctacctg cgccgcaccg cgacgcgcga catggagtac
     1021 ctgggccagc agatcggcaa gggcgacaag ctggtgtgca tactgggtgc gccgaaccgc
     1081 gacccggccc tgttcccgga ccccgacgcc ttcgacatca cgcgcccggt caacaacacg
     1141 cgcaagaact accgcacctt cggccagggt ccgcactact gcgtgggcgt gcaccaggca
     1201 cggctcaacc tcgaggtgat gctggaagag atcgtgcggc gcatgacgga cttccgcctg
     1261 ctcgccgcgc cgcgccacgc gcgctcgatc ttcatggagg ggtacaagga aatgcgcgtg
     1321 ggcttcgcga ggcgtgcggc ttgaacgaca tggaagcagc gcgcaaccta gcgcagcagg
     1381 atcagctgcg gccgcgcatc cggcccggcc cagaccagcg cgttctggcc ggatgaaccc
     1441 gcgatgagca tggcctcggc caggcccatg gcgggtacga acacactgtc ctcgccggcc
     1501 cattgcccgt cgggatcctc gccgacgccg ggaatgcagg cgtagccccg ggcgccgata
     1561 tcgcgcaaca gcgcggcctg agcggcccgg ttcgccgcgg cctcatgccg ttcgccgcgc
     1621 ggattccacg ccgtcacgaa agccgcgcaa tcgcagcccg agcgctcgaa gagtgccgcg
     1681 agcgcagcgc tgcgctcacc gatgcgcaac gtatacggcg gcggaccgag cacgcggtag
     1741 tggcagcgca ggtaggcgag cagcgtggct tcgccgatgg cgctcatggc tatctctcct
     1801 cttgcggcag acgtcccgtg ggtccggctt cagccggacg acgatgtccg gataaatccg
     1861 gacctacaag atccgctcga gcagcaggtc ggcggcggca tgcggatcga ggcgacaggc
     1921 ggcgacgtcg gcgagcacgg cagccgcatc cgggcgcgcg agcgcggccg ccaggatctt
     1981 cttcgccgcc gcgtcgcgca gcagcttcac gaacacctcg tgcgcgcggc gcgcgcgctg
     2041 tgccgcgaaa gcgccgctgg cgcgcaggaa ccccgcgtgc ctgtcgagca cgtcgacgat
     2101 ctgcgcgccg ccctcaccac gcgcggcaac ggcctgcagc accggcggtt tccaggcatc
     2161 cgcggccacc gcctgctcgc gcaggtgcag cgacatctcg agctggcgca gcgtctgcat
     2221 cgcgccgtcg cgatcggcct tgttcagcac gaagaggtcg cccgcctcca tcaggccggc
     2281 cttgatcgcc tggatctcgt cgcccagccc gggtgcggcg acgatgatcg tcgaatgcgc
     2341 gagcgcgacg atctcgatct cgtcctgccc cacgcccacg gtctcgacga agatcacctc
     2401 gtagcccatc gcgtccagca ccagcatcgc gtcgtagctg gcgcgcgcca gtccgcccag
     2461 ctgcccgcgc gtgcccatcg agcggatgaa cacgccctcg tccgcggcgt ggcgctgcat
     2521 gcgcacgcga tcgccgagaa tcgcaccgcc ggtgtaaggg ctggagggat cgacggcgac
     2581 cacgccgacg cgccgcccgc gccggcgctg ctcggtgatc agcatgtcga ccagcgtgga
     2641 cttgccgaca ccgcccggac cggtgacgcc gacgagatgc gcgcgcccgg tgtgcgggta
     2701 gacctgcccg agcgcggcgc gtccgcgcgg atcatcgtcc tcgagccagc ggatcagccg
     2761 cgcgccggcg gccacctggc cggccagcac gccggcgacc acgtccacgc tcagctagcc
     2821 cctgccgcgt cgcgcgcggc gcgccactcg ttcaggtacg cgacgatctc gcccgtatcg
     2881 gcgcccatcg tgaagatgcg cgccacgccg gccgcgcgca gcaccgcctg gtcctcctcg
     2941 ggaatcacgc cgcccacgat cagcagcttg tcgccgccgc ccttctggcg cagcaactcc
     3001 gccagcagcg ggatgttgcg catatgcccg gccgagagcg atgagacgcc gaccacgtcg
     3061 acgtcctcct ggatcgcggt gttggcgacc atctcgggcg tctgtttcag gccggtgaag
     3121 atcacctcca tcccggcgtc gcgcagcgcg tgcgcgatca cggtgacgcc gacggtatgg
     3181 gtgtcgagac cgagcttggc cagcagcacg cggatgggtg gctgcgccat gctcacaccc
     3241 ccgccgtctg gctgtcggcg tggtgcacgc cgaagacatc gcgcatcgca tcgcacagtt
     3301 cgccgtgcgt ggcgtagagt ttcaccgccg ccaggcaggg ctcgaccagg ttggtgccgg
     3361 tgcgcgcggc atcgcgcacc ttctccagcg cgagttgcac cgctgcattg tcgcgccgcg
     3421 cgcgcagcgc ctgcagcttc tgcacctgct gcgactccat ctcgtcttcg aggcgccaga
     3481 tctccatctc gcgcttcgcc tcaggtacca ccaggtgatt cacgccgacc agcttgcgcc
     3541 ggccctgctc catctcgcgc tggcgccggt actgctcgtt gcccagctcg cgctggaaat
     3601 agccctgctc gatggccttc agcgcgccgc ccatgttcat gatccgctcg atctcggcga
     3661 aggattcgtc ctcgatgcgc ctggtctgcg actcgacgta atacgatccg gccagcggat
     3721 cgatggtgtc ggccaccccg gtctcgtgcg cgaccaggtg ctggatcgcg gcacccacgc
     3781 ggatcgcgtc ctccgcgggc agcgcgtgcg cctcgtcgtg cagcggcgtg gtcatggtct
     3841 cgccggcccc ggccagcgcg caggcggtcg ccatgatcgc cagccgcccg atgttgttca
     3901 gcggctgctg cagcgtgagc gaactggtgc tcggcgatgt catcacgcgc agcttcatcg
     3961 cctcggggcg cgtcgcgccg tagcgttcct tcatgaggcg cgcccacacc ttgcggaagg
     4021 cgcgcagctt gcagatgccc tcgaagaaat ccatgtcgac gttgatcacg aagtggaagt
     4081 acggagccac cgtgtcgatg tcgaagccgc gcgcgagcat cgcgtcgatg taggccatcg
     4141 cgatcgtcac cgagaacgcg agctcctgca ccacgttcgc gcgcgccggc tgcagctgcg
     4201 cggcgatgat cgagatcggc tgccagtgcg ggtggtggcg cacgatgtac tcgaagcagt
     4261 cggtggcgat gcgcagcccg tgcccgggcg ggtagatgta gcgcccgacg caggtgtact
     4321 cgatcaggat gccgttgacg atgtgcagcg cgagctggcc cgggtcgaca cccttcttct
     4381 ccagcgccgc gatgatcatc gcgaggttca ccggctgcgg cgcgttgcac acgctcatca
     4441 ggtacttgag cttgtcgaag ggcaggtcga aggcttcctc caggtcctcg aggctgtcga
     4501 gcgccatgcc ggcacggccc acctcgcccg cgaccagcgg gttgtcgctg tcgtagccgt
     4561 tggtggtcgg caggtcgtac tcgaggatca ggccctgcag gccctggtcg agcatgtagc
     4621 gcgcgcgctt ggcccacgcc atgcccttgc cgaagccggt gacctgcgcc atggtccaca
     4681 gccgcgagcg gttgccgttg gcatccacgc cgcgcgtgaa cgggtactcg ccgggaaagc
     4741 cggcatccgt tgcgtaatcg aaaccgctgt ccgcgagatc gagcggcgtg tacagcgcct
     4801 tgatctccca gtccatggtc tgggtgcgga acgattcctt gcgctcgccc tgggcggcga
     4861 cgacggggcc gcgcgtcccg gcctgccagc gcgcctcctg ccgccgcaat atctccagcc
     4921 gcgccgcttc ggactgcacc gcgcccgcat ccggctcggc agttgttgct tgctcgattt
     4981 cttggacctt gcccatgcca gcgtctcccg acatccatcg gcgccatcat aggcacaacg
     5041 tccggtcgcg ccaatcgggg ctcgcggcga ggtctcgccc gccgtgagcc cccgcgcgaa
     5101 tcagaagttc ttgcgcacct gcagcgcgta ggtgcgcggc atgttgatcg ccccgaactg
     5161 tccgccctgc agcaggtcat tgtcctggct caggatcagc ttgcgttcgt cggtcaggtt
     5221 gttgcccttg agcgccacca tccagccgtt gtcggcgttg ctgaaagaca gtcgcgcatc
     5281 cagcagttcc aggctgtcgg cgccgaactc gttggagatc accgtggtgg actcgtcgcg
     5341 gaacgtgtac ccggtgtagg catccatgtc ccaaccgccg ccgactgcgt gcgtgaactc
     5401 gaggtagacc gtgcccgagt agtccggcgc gcgttgcagc gtctcgccgc ccaggtcctg
     5461 gccatccacg caacccgccg ggtctgccgc ccgcaggaag ggtccgcagg gcgcgttctc
     5521 gaagtcctcg tactcggcgt tcagccaggc gatgctgccg cccagcgtga gcgtatccgt
     5581 caggcgccac gcgccatcga gctcgatgcc cttggtgacg gcctcggc
//
LOCUS       DAORUQ010000192         5620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17292_length_5620_cov_9.244924, whole genome shotgun sequence.
ACCESSION   DAORUQ010000192 DAORUQ010000000
VERSION     DAORUQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17292_length_5620_cov_9.244924"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLW13_13555"
     CDS             <1..426
                     /locus_tag="PLW13_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71455.1"
                     /translation="SREALVDEVVREIGDAISQRLAQTQSAVPTVEEVLDAHLAALAE
                     HEVLYARLLGESATLPPAARARVFALQSGIAFRLRQAYERELAQGRVRAIDRTLLANT
                     WIALTNHYLMHRDLFSPGASVVAARRDELKACLLQLIRS"
     gene            438..716
                     /locus_tag="PLW13_13560"
     CDS             438..716
                     /locus_tag="PLW13_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY71456.1"
                     /translation="MSESHCVSCGSCGFPMQAPADFAGGMADSAYCSTCGDEFGRLRP
                     YDDVLQANAAYYEREQGLHPAAARELARALLASMPAWKSADPTRPLQS"
     gene            731..1624
                     /locus_tag="PLW13_13565"
     CDS             731..1624
                     /locus_tag="PLW13_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71457.1"
                     /translation="MRAPLLKSVLAIACTFAAHAAAAPVPAPELAEAQLRAINHRFVG
                     AFVDSDHDFMAGLVHDDFVRTASDGSWQDRDAFLATFREPARLSGASYDEVQVRLYGS
                     VAVTHAVFDALTRDGRARKVRYTDVYLWDGNDWRLVSGQNSLFQPEVPVGLQTGAEPP
                     HAPWTGSDPQGDDLAVLRDLNAAYVEAFRNADVAWYAAHLAPDYVVVSSDGSIKDRAA
                     ALADFARPVYAEHIKSFPVDKVRIRRFDDIALVHAENAFEMKDGRVGVNRYTDIWRKR
                     DGKWQCIAAHITVHKPLTLPG"
     gene            complement(1715..2461)
                     /locus_tag="PLW13_13570"
     CDS             complement(1715..2461)
                     /locus_tag="PLW13_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71458.1"
                     /translation="MMHLTRCIALLALSLGATLATAGETATVLITGANRGIGLEYARQ
                     FEAKGYTVIGTARNPGEAQELAALGVRIEALDVTDGASVAALAKRLEGVPIDVLINNA
                     GIGDRGDVDVLATDLALFERTLAVNTIGPLRVTQALLPNLRAGSRKHIVNMSSRLGSI
                     TLNNGNYPAYRASKSALNQVNRSLSIDLGKEGFVVVVVHPGWVRTEMGGAEAPLQPDE
                     SVRGLVALIEKLEAGNNGRFYDYLGAELPW"
     gene            2667..4691
                     /locus_tag="PLW13_13575"
     CDS             2667..4691
                     /locus_tag="PLW13_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY71459.1"
                     /translation="MSNRPGVLAAGLSVALAGAAGAAPVVAPYGSWASPVSAADLARS
                     AAGISDVRVFDGRIYWRQSRPAEGGRQVLVTFGADGLPKVLTPEGFSVRTRVHEYGGS
                     SYWVMGDAVVFANFSDQRLYLQRGDAAPVAITPAHYQYADCTIDGGRGALVCVREDHT
                     EATVKANGEERNEIVRVPVPAAPGPIDAGTVLVTGTDFVAYPRLSGDGKQLAWIEWNH
                     PSMPWDATTLKTAALDARGMPGKATIVAGGDRAVLEPQWGKDGTLYYIDEPSGWWNLY
                     ASRDGKTRALAPMAREFGGALWTLGNQTYVVADAGHVYARSSRETVDELARIDGKDGG
                     YRRIDAPFVTYGDLSLDRHGRLVAIASAATDEPVLVAIDPQDGQVQRLHQASTSPIPA
                     SLVSLGQPIRFPTTPGPAGEARIAHATFYPPNNPAFTGPEGSLPPLIVTIHGGPTSAS
                     RPVFSLSKQYWTSRGFAIVDVNYGGSTTYGRDYRKRLDRQWGVVDVQDAVAAVDFLVA
                     EGKADPARVAIRGGSAGGFTTLAALAFTNRFRAGANYFGVSDIAALAATSHKFESQYD
                     VSLIGPPDEAVYRARSPIFHLDGFTEPLITFQGAEDRIVTPDQSRKIVAALDQRGVMH
                     AYIEFAGEQHGFRKAESIVRAQEAELAFYGLVFGFTPADTIAPLDIRHPR"
     gene            4760..5245
                     /locus_tag="PLW13_13580"
     CDS             4760..5245
                     /locus_tag="PLW13_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71460.1"
                     /translation="MMKLRARIAVGLLGLLVATSLALPAMAAGEAEEPGKPSGPRSGT
                     VELASEQMRLIMGGTAGKGVLHFKGADYPFTFKTASAGIGAKAVTSVSATGDVYALER
                     LEDFAGKYTSITRSAMAGSREVTASYTNDKGVELHLRGTVEGVGLSLGGGVATIELIE
                     K"
BASE COUNT          867 a         1928 c         1965 g          860 t
ORIGIN      
        1 agtcgcgagg cgctggtgga cgaagtggtg cgggagatcg gcgacgcgat ctcgcagcgg
       61 ctcgcgcaga cgcaaagcgc cgtgccgacg gtggaggaag tgctcgacgc gcacctggcc
      121 gcactggcgg aacacgaggt gctctacgcg cgactgctgg gcgagtccgc cacgctgccg
      181 ccggcggcgc gcgcgcgcgt cttcgcgctg cagtcgggca tcgccttccg gttgcgccag
      241 gcctacgagc gcgagctggc gcagggcagg gtgcgcgcaa tcgatcgcac cctgctcgcc
      301 aatacctgga ttgcgctcac caaccattac ctgatgcatc gcgacctgtt cagtccgggc
      361 gcgagcgtgg tcgccgcgcg tcgcgacgag ctcaaggcct gcctgctgca actcattcga
      421 agctgaggag taccaccatg tccgaatccc attgcgtatc gtgcggatcc tgcgggttcc
      481 cgatgcaggc gccggccgat ttcgccggcg gcatggccga ctccgcgtac tgttccacct
      541 gcggcgacga gttcggcagg ctcaggccct acgacgacgt gctgcaggcg aacgccgcct
      601 actacgagcg cgagcagggc ctgcatccgg cggccgcccg cgagctcgcg cgcgcactgc
      661 tggccagcat gccggcatgg aaatccgccg atccgacccg gcccctgcaa tcctgaccga
      721 ggaatcgaac atgcgtgcac cgctgctcaa gtccgtgctg gccattgcct gcacattcgc
      781 cgcccacgcg gctgctgcgc ccgtgccggc gcccgaactg gccgaggcgc aactgcgcgc
      841 catcaaccat cgcttcgtcg gcgccttcgt cgacagcgac cacgatttca tggccggcct
      901 cgtccacgac gatttcgtgc gcaccgcgag cgatggttcg tggcaggacc gcgatgcctt
      961 cctggcgacc ttccgcgagc ccgcgcgact gtcgggtgcg tcctacgacg aggtgcaggt
     1021 gcgcctgtac ggatccgtcg ccgtgaccca cgcggtgttc gacgcgctga cccgggacgg
     1081 acgcgcccgc aaggtccgct acaccgacgt gtacctctgg gatggcaacg actggcggct
     1141 ggtcagcggg cagaactcgc tgttccagcc cgaggtaccg gtcgggctgc aaacgggcgc
     1201 cgaaccgccg cacgcgccct ggaccggcag cgatccgcag ggcgacgacc tcgccgtgtt
     1261 gcgcgatctc aacgcggcct acgtcgaagc gttccgcaac gccgacgtgg cctggtacgc
     1321 cgcgcacctc gcgcccgact acgtggtcgt gtccagcgac ggctcgatca aggatcgcgc
     1381 cgccgcgctg gccgatttcg cgcggccggt gtacgccgag cacatcaagt cgttcccggt
     1441 cgacaaggtc cgcatccgcc gcttcgacga catcgccctg gtccacgccg agaacgcctt
     1501 cgagatgaag gacggtcgcg tgggcgtcaa ccgctacacc gacatctggc gcaagcgcga
     1561 cggcaagtgg cagtgcatcg ccgcgcacat caccgtgcac aaaccgctga cgctgcccgg
     1621 ctgaagcgcg gcccggatcc ggcggccggc cggccaggca tgcgccggcc ggggcaatgc
     1681 cgagcgaagc atccggcccc ggttggcggg ccggctacca gggcagttcg gcgccgagat
     1741 agtcgtagaa gcggccgttg ttgccggcct ccagcttctc gatcagggcc accagtccgc
     1801 gcacgctttc gtcgggctgc agcggggctt cggcaccgcc catttcggtg cgcacccagc
     1861 ccggatggac gaccaccacc acgaagcctt ccttgcccag atcaatcgac aggctgcggt
     1921 tgacctggtt cagcgcggac ttgctggcgc ggtaggccgg gtaattgccg ttgttcagcg
     1981 tgatgctgcc caggcgcgag gacatgttga cgatatgctt gcggctcccg gcgcgcaggt
     2041 tcggcagcag ggcctgggtt acccgcagcg gcccgatcgt gttgaccgcc agcgtgcgct
     2101 cgaacagcgc gaggtcggtc gccaggacgt cgacatcacc gcggtcgccg atgccggcgt
     2161 tgttgatcaa cacatcgatg ggcacgcctt cgaggcgctt ggcgagggcc gcgacgctgg
     2221 cgccgtcggt cacgtccagc gcctcgatcc ggacgccgag cgccgccaac tcctgcgcct
     2281 cgccgggatt gcgcgctgtg ccgatcacgg tgtagccctt ggcctcgaac tggcgcgcat
     2341 actcgaggcc gatgccgcgg ttggcgccgg tgatcagcac ggtggcggtc tcccccgcgg
     2401 tcgcgagggt ggcgcccagc gagagcgcga ggagggcaat gcatcgagtc aggtgcatca
     2461 tggcgtctgg ctccggagcg aggagaggtg ggtcccgcga ctgtagccca cgccgggaaa
     2521 ttgcggaaag ctgcggcgcc gcgccggtgg ctgtcgccgc gcggcacctt cagtgcgggt
     2581 gccctgcgcg gtatcgtcgg caggtggcgg cgccgatgcg tgcacggcga cgccgggatc
     2641 cgacccttcc tgcattggag ttgacgatgt cgaaccgtcc tggggtgctc gctgccggac
     2701 tttcggttgc gctggcgggc gcggccggcg ccgcgcccgt ggtggcgccg tatggaagtt
     2761 gggccagccc ggtttccgcg gcggacctcg cccggtcggc ggcgggcatc agcgatgtgc
     2821 gggtgttcga cgggcgcatc tactggcgcc agagccggcc ggccgagggc ggacgccagg
     2881 tgctggtcac gttcggggcc gatggcttgc cgaaggtgct cacgccggaa ggattcagcg
     2941 tgcgcacgcg cgtgcatgag tacggcggca gcagctactg ggtcatgggc gatgcggtgg
     3001 tcttcgcgaa cttcagcgac cagcgcctgt acctgcaacg tggcgacgcg gcacccgtgg
     3061 cgatcacgcc cgcgcactac cagtatgcgg actgcaccat cgatggcggg cgcggcgcgc
     3121 tggtatgcgt gcgcgaggac cacaccgagg ccaccgtgaa ggccaatggc gaggagcgca
     3181 acgagatcgt ccgcgtgccc gtgccggcgg cgcccggccc gatcgacgcc ggcacggtgc
     3241 tggtcaccgg caccgatttc gtcgcctacc cgcgcttgag cggcgacggc aagcagttgg
     3301 cctggatcga gtggaaccac ccgtccatgc cgtgggacgc gaccacgctc aagaccgcgg
     3361 cgctcgacgc ccgcggcatg ccgggcaagg ccaccatcgt ggccggcggc gaccgcgccg
     3421 tgctcgagcc gcagtggggc aaggacggca cgctctacta catcgacgag cccagcggct
     3481 ggtggaacct gtacgcaagc cgcgacggca agacccgcgc gctggcgccg atggcgcgcg
     3541 agttcggcgg tgcgttgtgg acgctgggca accagaccta cgtggtggct gatgccggcc
     3601 acgtctacgc ccgcagcagc agggagacgg tcgacgagtt ggcccggatc gacgggaagg
     3661 acggtgggta tcgccgcatc gacgcgccct tcgtgacata cggcgatctt tccctcgatc
     3721 gccacggacg cctggtcgcc atcgccagcg ccgccacgga cgagcccgtg ctggtggcga
     3781 tcgatccgca ggatggccag gtgcagcgcc tgcaccaggc cagcacgagt ccgattccgg
     3841 cgtcgctggt ctcgctcgga cagcccatcc ggtttcccac gacgcccggt cccgcgggcg
     3901 aggcgcgcat cgcgcatgcc acgttttatc cgcccaacaa ccccgcgttc accgggccgg
     3961 agggcagcct gccgccgctg atcgtcacca tccatggcgg acccacgtcg gcgtcgaggc
     4021 cggtgttctc gctatcgaag cagtactgga ccagccgtgg cttcgccatc gtcgacgtga
     4081 actatggtgg ctcgaccacc tacgggcgcg attaccgcaa gcgcctcgat cgccaatggg
     4141 gcgtggtgga cgtgcaggac gcagtggccg cggtggattt tttggtggcc gaaggcaagg
     4201 ccgacccggc ccgggtggcc atccgcggtg gcagcgcggg cggtttcacg acgctggccg
     4261 cgttggcctt caccaaccgc ttccgcgccg gcgccaacta cttcggcgtg tccgacatcg
     4321 cggcgctggc cgccaccagc cacaagttcg agagccaata cgacgtctcg ctgatcggcc
     4381 cgccggacga ggccgtctac cgggcgcgct cgccgatctt ccacctcgac ggcttcaccg
     4441 agccgctcat caccttccag ggcgccgagg accgcatcgt cacgccggac cagtcgcgca
     4501 agatcgtcgc cgcgctggac cagcgcggcg tgatgcatgc ctacatcgag ttcgccggcg
     4561 aacagcacgg attccgcaag gccgagagca tcgtccgcgc gcaggaggcc gagctggcct
     4621 tctacggcct ggtgttcggc ttcacccctg ccgacaccat cgcgccgctc gacatccggc
     4681 atccacggta accgcgcgca tttgcccggc tggtccggtc ggactaggct ggcgggcgag
     4741 taccacggga gaaacggtca tgatgaaatt gcgtgcgcga atcgcggttg gattgctggg
     4801 cctgttggta gcgacatcac tggcactgcc ggcgatggct gccggcgagg ccgaggaacc
     4861 gggcaagccc agcggcccgc ggagcggaac ggtcgaactc gcgtccgagc agatgcgcct
     4921 gatcatgggg ggcactgctg gcaagggcgt attgcacttc aagggcgccg actatccctt
     4981 caccttcaag actgcgagcg cggggatagg cgcgaaggcg gtgacctcgg tgagcgcgac
     5041 gggcgacgtg tatgccctcg aacggctcga ggatttcgcc ggcaagtaca cctcgatcac
     5101 ccgtagcgca atggccggat cgcgcgaggt gaccgcgtcg tataccaacg acaagggcgt
     5161 cgagctccac ttgcggggaa ccgtcgaggg cgtgggactc agcctgggtg gaggcgttgc
     5221 cacgatcgag ttgatcgaga aatagccggc tgtacccgag gcaggggggt ggccctggct
     5281 cccggcgtgg cactggacgg gtccggggcc cgggcaggca tcccagcccc aaagggcagc
     5341 gcgccaactg ccgcagcggc cgattcgcat gcagaatcgg tggcaggcgc atcgcaccga
     5401 tcgatgcggc cgggttgcga gcggtcgacg cggcaccttg gctccgttct tccgccgctc
     5461 gatcgggtgc gaatcgtggg ctaccgctcg gatatgccgc aagctcgtct tgaatccagc
     5521 caagcgtggc gcaagcgtct gccaaccatg cgcgcgcacg gtcgtgtcct catgcttgcg
     5581 ctcgtgcttg cgacagcagg ctgcgggccg gcaccggaga
//
LOCUS       DAORUQ010000193         5601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17375_length_5601_cov_8.406419, whole genome shotgun sequence.
ACCESSION   DAORUQ010000193 DAORUQ010000000
VERSION     DAORUQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17375_length_5601_cov_8.406419"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PLW13_13585"
     CDS             complement(<1..1130)
                     /locus_tag="PLW13_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY71461.1"
                     /translation="METHYRACNLCEAICGLEIGVEAGRVVSIRGDAADPLSRGYLCP
                     KATALADLQDDPDRLRRPLLREGDNWREIGWEEAFAIAAERLQAVRERHGADAVGVYR
                     GNPSVHNWGLMTHANHFLKRLGTRNHFSATSADQLPHHLVCLWMYGHQFLQPVPDIDR
                     TQFLLLIGYNPVASNGSMMTVPDIRGRLKELRARGGRLVVIDPRRSETAEVADRHHFI
                     RQGSDAALLLALLHVLFDEGLARTDGLQSQARNLAAVPPLLARFTPDFAAGATGIEAG
                     AIRALAREFAAAPSAACHGRMGVSTQRFGTLCQWAIQLLMLFTGNLDRPGGALLTHSA
                     VMGAGPDDNPKGAFARRASRVSGYPEFGGEFPVAALAEEMLTP"
     gene            1325..3514
                     /locus_tag="PLW13_13590"
     CDS             1325..3514
                     /locus_tag="PLW13_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY71462.1"
                     /translation="MQDSVHGICRSCAAMCPIVIDREDGRPVRIIGDKHNPVYWGYTC
                     IKGREMVNQLRHPDRLLGSLRREVDGSFTPIASAQALDEVTQKLQRIIAEHGPRAVAT
                     YAGTYIFTYPATQPVSTAWMNAIGSPMMFTSSTIDQPGKAIAAALHGTWSAGPQVFDD
                     ADTWLLVGVNPIVAHSGGVPNQNPAKRLKDAVERGMRPIVLDPRRTECAERAFVHLQA
                     RPGEDPAVLAGMIRVILKESLFDAGFVRAHVAGVDALAAAVEPFTPQYVERRAGVPMA
                     DLVLAARTFASAGRGGATAGTGSNMAPRGNLTEYLLLCLMSLCGRWLKAGERVPNAGV
                     LGPRFAPRAQANAPWPAWGYGEKLRVRGFSDAACGLPTSALPDEILLPGEGQVKALIS
                     IGGNPLMAWPDQKKTLAALKALDLFVCLDIQMAHNSCHLAHYNIGCKHSLESPAITLP
                     NEMLSYFGTGFGYSVPYAQYAPPATEPPPGADLIEEWEFFYEVARRMKLELDMAVAYS
                     WTTTSGEPARYRFDMARKPTTDELFEVLCAEGRVPFARVKERPGGRVFDDEAITVMPA
                     EAGHAARLQVGDGAMVAELHAVAAEPIEHEALREYPFRLISRRMHGVMNTTGRNNARQ
                     MRQRHHNPAFMNPLDLAELGLHEGDIVRIASRYGEIPAIVEAEDGIRRGVISMSHCFG
                     GTDPDSPSDVREAGANTGLLASVEHEYDPYSGIPRMSAIPVRIGKGG"
     gene            complement(3528..4067)
                     /locus_tag="PLW13_13595"
     CDS             complement(3528..4067)
                     /locus_tag="PLW13_13595"
                     /inference="COORDINATES: protein motif:HMM:NF021360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HQY71463.1"
                     /translation="MRNSLQDQLLKAGLVDAKKLKESNRASDKQKRQAGKNAAELDAQ
                     RRLAEQALRDKAEADRALAQQHNAELRQREIAAQIAQLVRHYRQPREGGDIAYNFADR
                     GVIKKLYVSARLRDQLIRGQLVIVRSGNGYELVPVACAERIRARDADAIVVWNTPAAD
                     QPAEDDPYKDFPIPDDLMW"
     gene            complement(4085..5086)
                     /locus_tag="PLW13_13600"
     CDS             complement(4085..5086)
                     /locus_tag="PLW13_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HQY71464.1"
                     /translation="MSARSTAAAPPDDALARVAARYAVAITPAMEALIDPQDPLDPIA
                     LQFRPDARELITLLDENPDPIGDRAHAPVAGIVHRYPDRCLLKLVHVCAVYCRYCFRR
                     EMVGPTGDGTLGPAELDAAIAYIAAHPEIWEVVLTGGDPFVLSPRRLLEVTQHLAAIP
                     HLKVLRWHTRVPVVDPARVTPELVAAISSPHATTYVVLHANHPRELTTQARDAIALLA
                     DAGIPLLAQTVLLKDVNDDAGTLEALMRALVESRIRPYYLHHLDKAPGTSHFRCTIEH
                     GRELVRQLRERASGICQPTYVLDIPGGHGKTPIAESGLRETQTGYEVRDRHGEWRPA"
BASE COUNT          835 a         1894 c         2013 g          859 t
ORIGIN      
        1 ggcgtcagca tctcctcggc gagtgctgcg accgggaact cgccaccgaa ctcgggatag
       61 ccgctgaccc ggctcgcgcg gcgcgcgaag gcccccttgg ggttgtcgtc cggtccggcg
      121 cccatcacgg cggagtgggt gagcagcgcg ccgcccgggc ggtcgagatt gccggtgaac
      181 agcatcagca gctggatcgc ccactggcac agcgtgccga agcgctgcgt cgagacaccc
      241 atgcgcccgt ggcaggcggc cgagggcgcg gcggcgaatt cgcgcgccag cgcgcggatc
      301 gcgccggcct cgatgccggt cgcgcccgcc gcgaaatccg gcgtgaagcg cgccagcaat
      361 ggcggcaccg ccgccaggtt cctcgcctgc gactgcagcc cgtccgtgcg cgccaggccc
      421 tcgtcgaaca gcacgtgcag cagcgcgagc agcagtgcgg catcgctccc ctggcggatg
      481 aagtggtgac ggtccgcgac ctcggccgtc tcgctgcggc gcgggtcgat caccaccagc
      541 cggccgccgc gtgcgcgcag ttccttcagt cgcccacgga tatcgggcac ggtcatcatg
      601 ctgccgttgg aggccacggg gttgtagccg atcagcagca ggaactgcgt gcggtcgatg
      661 tcgggcacgg gctgcaggaa ctggtgcccg tacatccaca ggcacaccag gtggtgcggc
      721 agctggtcgg ccgaggtggc ggagaaatgg ttgcgcgtgc cgaggcgttt caggaagtgg
      781 ttcgcgtgcg tcatcagtcc ccagttgtgc accgaggggt tgccgcggta cacgccgacc
      841 gcgtcggcgc cgtggcgctc acgcaccgcc tgcagccttt cggcggcgat cgcgaaggcc
      901 tcctcccagc cgatctcgcg ccagttgtcg ccttcgcgca gcagcgggcg gcgcagccgg
      961 tcgggatcgt cctgcaggtc ggccagggcg gtggccttgg ggcagaggta gccgcggctg
     1021 agcgggtcgg cggcatcgcc gcggatcgag accacgcgcc cggcctcgac gccgatctcc
     1081 aggccgcaga tcgcctcgca caggttgcag gcgcggtagt gcgtttccat ggccggcttc
     1141 cctgggtggt acgggcagga cgctatcacg cttccggagc ggctccaatt acagcggccg
     1201 gaacacgccg ggtttccccc gggcacgcgg atgcagcccg gcagggtccg tccggcgatt
     1261 gccgtggacg gcgtccgggc ataacatcgc cccataaaac catccgatga aatggcgatt
     1321 tcccatgcaa gacagcgtcc acggcatctg ccgctcctgc gccgcgatgt gcccgatcgt
     1381 gatcgatcgc gaggacgggc ggccggtgcg gatcatcggt gacaagcaca acccggtcta
     1441 ttggggctat acgtgcatca agggccgcga gatggtgaac cagttgcggc atcccgaccg
     1501 cctgctcggc agcctgcggc gcgaggtcga cggcagtttc acgccgatcg ccagtgcgca
     1561 ggcgctcgac gaggtgacgc agaagctgca gcgcatcatc gccgagcacg gtccgcgcgc
     1621 cgtcgcgacc tatgccggca cctacatctt cacctacccc gcgacgcagc cggtgtcgac
     1681 ggcgtggatg aacgcgatcg gctcgccgat gatgttcacc tccagcacca tcgaccagcc
     1741 cggcaaggcc atcgccgcgg cgctgcacgg cacctggagc gcgggcccgc aggtgttcga
     1801 cgatgccgac acctggctgc tggtgggggt gaacccgatc gtggcgcatt cgggcggggt
     1861 gccgaaccag aatcccgcga agcggctgaa ggacgctgtc gagcgcggca tgaggcccat
     1921 cgtgctcgat ccgcgccgca ccgagtgcgc cgagcgcgcc ttcgtgcacc tgcaggcccg
     1981 ccccggcgag gatcccgcgg tgctggcggg catgatccgc gtgatcctga aggagtcgtt
     2041 gttcgatgcc gggttcgtgc gcgcgcacgt cgccggcgtc gacgcgctgg ccgccgcggt
     2101 cgagcccttc acgccgcagt acgtggagcg gcgcgccggt gtcccgatgg ccgatctcgt
     2161 gctggcggcg cgcaccttcg ccagcgccgg gcgcggcggc gcgaccgccg gcaccggctc
     2221 gaacatggcg ccgcgcggca acctgaccga gtacctgctg ctgtgcctga tgagcctgtg
     2281 cggacgctgg ctgaaggcgg gcgagcgcgt gccgaacgcg ggcgtgctgg ggccgcgctt
     2341 cgcgccgcgc gcgcaggcca acgcgccgtg gccggcctgg ggctacggcg agaagctgcg
     2401 cgtgcgcggc ttcagcgacg ccgcctgcgg cctgccgacc tcggcgctgc ccgacgagat
     2461 cctgctgccc ggcgaggggc aggtgaaggc gctgatctcg atcggcggca acccgctgat
     2521 ggcctggccg gaccagaaga agacgctcgc ggcgctgaag gcgctcgatc tattcgtctg
     2581 cctcgatatc cagatggcac acaacagttg ccacctggcg cactacaaca ttggctgcaa
     2641 gcacagcctc gagagccccg cgatcacgct gccgaacgag atgctcagtt atttcggcac
     2701 cggctttggc tactcggtgc cctacgcgca gtacgcgccg ccggcaaccg agccgccgcc
     2761 cggtgccgat ctcatcgagg agtgggagtt cttctacgaa gtggcgcggc gcatgaagct
     2821 cgagctggat atggcggttg cctactcctg gaccaccacc agcggcgagc cggcgcgcta
     2881 tcgcttcgac atggcgagga aacccacgac ggacgagctg ttcgaggtgc tgtgcgccga
     2941 ggggcgcgtg cctttcgcgc gcgtgaagga gcgccccggg ggacgcgtgt tcgacgacga
     3001 ggcgatcacg gtgatgcccg ccgaggcagg gcacgcggcc cggctgcagg tcggcgacgg
     3061 ggcgatggtc gccgagctgc acgcggtggc ggccgagccg atcgagcacg aggcgcttcg
     3121 cgagtacccg tttcgcctga tcagccggcg catgcacggg gtgatgaaca ccaccgggcg
     3181 caacaacgcg cggcagatgc gccagcggca ccacaacccg gcgttcatga acccgctcga
     3241 cctggcggaa ctggggctgc acgagggcga catcgtccgc atcgcctcgc gctacggcga
     3301 gataccggcc atcgtggagg cggaggatgg catccggcgc ggcgtgatct cgatgagcca
     3361 ctgtttcggc ggcaccgacc cggacagccc ttccgacgtg cgggaagcag gtgccaacac
     3421 cggcctgctc gccagcgtcg agcacgaata cgacccgtac agcggcattc cgcgcatgag
     3481 cgcgatcccg gtgcgcatcg gcaagggcgg ctagcggcgc gcgcgcctca ccacatcagg
     3541 tcgtcgggga tcgggaaatc cttgtaggga tcgtcctcgg ccggctggtc ggcggcgggg
     3601 gtgttccaca ccacgatcgc atcggcatcg cgcgcgcgga tgcgctcggc gcacgcgacg
     3661 ggcaccagct cgtagccgtt gcccgagcgc acgatcacga gctggccgcg gatcagctgg
     3721 tcgcgcaggc gcgcgctgac gtagagcttc ttgatgacgc cgcggtcggc gaagttgtag
     3781 gcgatgtcgc cgccctcgcg cggctggcgg tagtggcgca ccagctgcgc gatctgcgcg
     3841 gcgatttcgc gctggcgcag ctccgcgttg tgctgctgcg cgagcgcgcg atcggcctcg
     3901 gccttgtcgc gcagcgcctg ttccgcgagg cgtcgttgcg catcgagctc ggcggcattc
     3961 ttgccggcct gcctcttctg cttgtcgctg gcgcggttgc tctccttgag cttcttcgcg
     4021 tcgaccagtc cggccttgag aagctggtcc tgcagggaat tgcgcatgct gtactccgtt
     4081 cctgttatgc ggggcgccac tcgccgtggc ggtcgcgcac ttcgtagccc gtttgcgttt
     4141 cgcgcaggcc ggattcggcg atcggggtct tgccgtgccc gcccgggatg tcgagcacgt
     4201 aggtcggctg gcagatgccc gaggcgcgct cgcgcagttg ccgcaccagt tcccgtccat
     4261 gctcgatggt acagcggaaa tgcgaggtgc ccggtgcctt gtccagatgg tgcaggtaat
     4321 agggcctgat ccgcgattcc accagcgcgc gcatcagcgc ctccagcgtg ccggcgtcgt
     4381 cgttcacgtc cttcagcagc acggtctgcg ccagcagcgg gatgccggca tcggcgagca
     4441 gtgcgatcgc atcgcgcgct tgcgtggtca gctcgcgcgg gtggttggcg tgcagcacca
     4501 cgtacgtggt ggcgtgcgga ctgctgattg ccgcgacgag ctccggtgtg acgcgtgccg
     4561 gatccaccac cggcacgcgc gtgtgccagc gcagcacctt caggtgcggt atcgcggcga
     4621 gatgctgcgt gacctcaagc agccggcgcg gcgaaagcac gaaggggtcg ccgccggtca
     4681 gaaccacttc ccagatttcg gggtgcgccg cgatgtaggc gatcgcggca tccagttccg
     4741 ctggcccgag cgtgccgtcg ccggtcgggc cgaccatctc gcggcgaaag cagtagcggc
     4801 agtacaccgc gcagacgtgc accagcttca gcaggcagcg gtcggggtag cggtgcacga
     4861 tgcccgcgac cggcgcgtgc gcgcggtcgc cgatcgggtc ggggttctcg tcgagcagcg
     4921 tgatcagctc gcgcgcatcg gggcgaaact gcagcgcgat cggatccagc ggatcctgcg
     4981 ggtcgatcaa cgcttccatg gccggcgtga tcgccaccgc atagcgcgcc gcgacgcgcg
     5041 cgagggcgtc gtcgggtggc gcagcggcag ttgatcgggc ggacatgcgt ggcgaacccg
     5101 tggtcggcgt gaggaaggcg gcaattatcc gcgatcggag gcggggcgag gcgggcgatt
     5161 cggcggaatt gtccgcaaat ccccgagggt ccggacgatt gcccggctga agagggtgtc
     5221 gcaaaaccat ctgcgctgtg gatagcggcc cgacaatccc gcgtatcgcg agccatcggc
     5281 gctcgagcac ccatcggtgc cgcggacacg ccgtgaatac atccctgtag gctacctgtc
     5341 gccgtcctgg cgacagagtg tccgccgcac cgatgggtgc tcgagcccct cgccgtggtg
     5401 gaaaccctgg aaaatgcatg cattcgcgga tctgcccgat ggacacgcgc tcaaccgagc
     5461 ggctgtgctg cccgctgttg gcttgcgggg ccttgcgccg caggacgcgg cgcggagcta
     5521 cagggacgta ctcgtgcgtg ccccgcagga caatggcggg cagcacagcc agcgacgcac
     5581 caagccgctc gctcgcaacg t
//
LOCUS       DAORUQ010000194         5540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_17647_length_5540_cov_5.633181, whole genome shotgun sequence.
ACCESSION   DAORUQ010000194 DAORUQ010000000
VERSION     DAORUQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17647_length_5540_cov_5.633181"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..534)
                     /locus_tag="PLW13_13605"
     CDS             complement(160..534)
                     /locus_tag="PLW13_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71465.1"
                     /translation="MQLKDKVAVITGAAGGLGRGIGRRFAREGARLVVCDIDESGLAP
                     LREEIEAAGGECLALPCDVSSSASVDAMFARTDARFGTVHILVNNAALVPDKPHDEER
                     RKRHYALLAVMFATETMAWPCR"
     gene            complement(553..1878)
                     /locus_tag="PLW13_13610"
     CDS             complement(553..1878)
                     /locus_tag="PLW13_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71466.1"
                     /translation="MAFHHAPAHGAHAGSSGPPTLAAWIAFALIFGLMLSDYLSRQVI
                     NAVFPFIKAQWSLSDTQLGSLVSIVAVTVGVFSFPVSVLADRFGRVLCVTIMAVLWGL
                     ATLLCGLAGSFTILLFARLLVGLGEAGYSSAGGAILLGAFPARLHATVMGAFLAASLF
                     GSVLGVVLGGVLAASYGWRTAFVVVGAGGLLLALVFPLFVKERPDLTREAAARAREEA
                     IALRDLPREILSARTAAWSYLGSGLQMFSVGAMLAWIPSFLNRHHGMPPAEAALAAGV
                     LTLLAGIGMVLGGVLVDVVARRAARNRPLVVACYAFLSCVLFVLAFRYCTGTLQLLVI
                     GAGMFLAGAHAGPTGAIATSVTSPRLHATVLATGTLANNIFGLAPGPVVTGLLADRFD
                     LQTALGLVPLPSLLAAACFVMARRHHDRDVARIADAHPSSSIEALEEIA"
     gene            complement(1886..3520)
                     /locus_tag="PLW13_13615"
     CDS             complement(1886..3520)
                     /locus_tag="PLW13_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY71467.1"
                     /translation="MLQWARNYRRDQLAGDLGAGAIVALMLIPQGMAYALLAGLPPAA
                     GLYASILPPVAYALFGSSMTQSVGPMAITSLMSAAALSALAPAGSVLYVTLATQLALL
                     TGLVLLACGALRLGFLSGFLSRPVLAGFTAGAAILIAVGQLPLLLTDAAAHPDVPTAL
                     IGGVSLALLWLAKGPLLRRTGEGSPWPVLLRLVPAAVIGAATLAAWILELDRSGVRVL
                     GSIPRGLPPLGLQYSGAHWQSLLAPALLIGFVVFVSSQSAAQSLAQRRGERVLPDGEL
                     LGLGAANVAGALSGGFPVTGSISRSAVNDAAGANTPLASLVSAGLLAAVLSLPTGWLA
                     PLPLAALAATIIVAVAGMIDIAALKEAWRYDRADAMAMIVTAAGVLALGVERGVIIGV
                     VVSLGALIARASRPNIAVVGRAPGSDFFRNVGRHEVETLPEVLMLRIDAAIWFGNTDA
                     ILGRVELELASRPATRHVVLVMSAVNHIDVTGVLMLRELNLSLAGRNILLHLAKTKES
                     VMDRLASSQLPAELRGRMFASPGQAFRALECGASGG"
     gene            complement(3551..4597)
                     /locus_tag="PLW13_13620"
     CDS             complement(3551..4597)
                     /locus_tag="PLW13_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQY71468.1"
                     /translation="MHYLKDSLLPENQEKLLITAAPYGPGFLPGDSDDIPVTMDEQVQ
                     KAVDCFNAGAQILHIHVREDDGSGSKRLSKFEELIARLRQAVPGMILQVGGSISFAPE
                     PGKEAKWPGNDSRHALAQLEPKPDQVTIAIGTSGMNIVDLMSERDVAGTSLAAPEMQA
                     AYRDMYLEAGPTFYETNLKALRDNGVQAYFMLGHVHQLETVETLIRTGVYMGPLNLTY
                     VAIGGGLAARHPSDLVEFAVRTPDGAVLTNESLMRTMHPTNAIAIALGHHVRVGIEDN
                     LWGPRHERMSTVQQIEWCVAQARHLGRDVATAGEARETYRIGTYYDSVDETLRALGMP
                     PNRLPGQRGRPIWR"
     gene            complement(4637..5029)
                     /locus_tag="PLW13_13625"
     CDS             complement(4637..5029)
                     /locus_tag="PLW13_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY71469.1"
                     /translation="MHDDSPTPVSARMRAAGNWNPAWDVPAQLDAAYVEKFLAMGAHA
                     MGRGTLDPLTIEFIAIAVDASCTHLYAPGVRRHIRKALELGATRDQVMTVLELVSVLG
                     IHSVALGAPMLIEEAAALATEGPVKGTF"
     gene            5167..>5540
                     /locus_tag="PLW13_13630"
     CDS             5167..>5540
                     /locus_tag="PLW13_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQY71470.1"
                     /translation="MPFTHVRSASLTGYAEVARSVGLDPFARLRAAGLDVRCLDDPDL
                     KIPVLRLHALLESSAHAAGIEDFGLRMAMSRRLSNLGLISLAAREEPTVRDALHCLQR
                     TMHLHNEAILIDIEEANGIVVIR"
BASE COUNT          899 a         1895 c         1949 g          797 t
ORIGIN      
        1 cggcaccacc gattccaatc cgggcgatgc caatgactgg cggcattatc gctatcgcgt
       61 ttacgacaag atcattcccc tgcgcaacat catctggggc accgcgccat gaacatcacg
      121 caccattcca ccgcgacagg ccttcaaccc ggaccggatt caacggcaag gccacgccat
      181 cgtctctgtg gcaaacatca ccgccagcag ggcgtagtgg cgcttgcgcc gctcctcgtc
      241 gtgcggcttg tccggcacga gggccgcatt gttcaccagg atgtgcacgg tgccgaagcg
      301 cgcgtcggtc cgcgcgaaca tcgcgtcgac gctggcgctg gaggatacgt cgcagggcag
      361 cgcgaggcac tcgccccccg ccgcttcgat ttcctcgcgc agcggcgcca gcccggattc
      421 gtcgatgtca cagacgacga gccgggcgcc ctcgcgcgcg aagcgccgcc cgatgccgcg
      481 cccgagtccg cccgccgcgc cggtgatcac ggctaccttg tccttcagtt gcatctgccg
      541 attcctcctc gttcacgcga tttcttccag cgcctcgatg ctgctcgacg gatgcgcgtc
      601 ggcgatgcgc gccacatcac ggtcatggtg gcgccgggcc atcacgaagc aggccgccgc
      661 cagcaggctg ggcagcggca ccagcccgag ggcggtctgc agatcgaagc ggtccgccag
      721 caggccggtc accaccggcc cgggcgccag cccgaagatg ttgttcgcga gcgtgccggt
      781 ggcgagcacc gtcgcgtgga gacgcggact cgtgacggat gtggcaatgg cgccggtcgg
      841 cccggcgtgc gcgcccgcca aaaacatccc ggcgccgatc acgagcagct gcagcgtccc
      901 ggtgcaatag cggaaggcaa gcacgaagag cacgcagctg aggaaggcgt agcacgcgac
      961 cacgagcggc cggttgcgcg ccgcgcgccg cgcgaccacg tcgaccagca cgccgccgag
     1021 caccatcccg attcccgcga gcagggtcag cacgcccgcg gcgagggccg cctccgcggg
     1081 cggcatcccg tgatgacgat tgagaaaact cgggatccac gccagcatcg caccgacgct
     1141 gaacatctgc aggccgctgc cgaggtagct ccacgccgcc gtgcgcgccg agagaatctc
     1201 gcgcggcagg tcgcgcaggg cgatcgcctc ctcgcgcgcg cgtgccgcgg cttcccgcgt
     1261 gaggtccgga cgctccttga cgaacagcgg gaacacgagc gcgagcagca ggccgccggc
     1321 accgaccacg acgaacgccg tgcgccagcc gtagctcgcg gcgagcacgc cgccgagcac
     1381 cacgccgagt accgagccga acagcgaggc cgccagaaag gcgcccatga ccgtggcgtg
     1441 gaggcgcgcg gggaatgcgc cgagcaggat ggcgccgccc gcgctgctgt agccggcctc
     1501 gccgaggccg accagcagcc gcgcgaagag caggatcgtg aagctgccgg ccagcccgca
     1561 gagcagcgtc gccagtcccc agaggaccgc catgatcgtg acgcagagca cgcggccgaa
     1621 gcggtcggcg agcacggaga cggggaagct gaacacgccg acggtgactg ccacgatgct
     1681 caccagcgag ccgagctgcg tgtcgctcag gctccactgc gccttgatga acgggaagac
     1741 cgcgttgatc acctggcgcg acaggtagtc cgagagcatc agcccgaaga tcagtgcgaa
     1801 ggcgatccac gcggccagcg tgggcgggcc cgaggagccg gcgtgcgcgc cgtgagcggg
     1861 tgcgtggtgg aatgccatgg ccgcgctacc cgccgctcgc gccgcattcg agcgcgcgga
     1921 aggcctgccc cgggctggcg aacatccgcc cgcggagttc ggccggcagc tggctggagg
     1981 cgagccggtc catcacggat tccttggttt tcgccaggtg caggaggatg tttcttccgg
     2041 ccagcgaaag gttcagttcg cgcagcatca gcacgcccgt cacgtcgatg tgattgaccg
     2101 ccgacatgac cagcaccacg tggcgggtcg ccgggcgcga tgccagctcg agttccacgc
     2161 ggccgaggat cgcatcggtg ttgccgaacc agatcgccgc gtcgatgcgc agcatgagca
     2221 cctcgggcag cgtctccacc tcgtggcgcc cgacgttacg gaagaaatcg ctgcccggtg
     2281 cccggcccac caccgcgatg ttcgggcggc tggcgcgcgc gatcaacgcg cccagggaca
     2341 ccaccacgcc gatgatgacg ccgcgctcga cgcccagcgc cagcacgccg gccgcggtga
     2401 cgatcatcgc catggcatcg gcgcggtcgt atcgccaggc ttccttcagc gccgcgatgt
     2461 cgatcatgcc ggcaacggcc acgatgatcg tcgccgcgag cgccgcgagc ggcagcggtg
     2521 ccagccatcc cgtcggcagg ctgaggacag cggcgagcag gcccgcactg acgaggctcg
     2581 ctagcggcgt gttcgcgccg gctgcgtcgt tcaccgcgga acgggaaatg ctcccggtga
     2641 cggggaatcc gcccgagagc gcgccggcaa cgttggcggc gccgaggccc agcagttcgc
     2701 cgtcggggag cacgcgctcg ccgcgccgct gcgccagcga ctgggcggcg gactggctgg
     2761 agacgaagac cacgaagccg atcagcagcg cgggcgcgag caggctttgc cagtgcgcgc
     2821 cggagtactg caggccgagc ggtggcaatc ctcgcggtat gctgcccagc acgcgcacgc
     2881 cgctgcggtc gagctccagg atccacgccg cgagcgtggc agcgcctatc acggcggccg
     2941 gcacgagcct gagcagcacg ggccaggggc tgccttcacc cgtgcggcgc aacaaggggc
     3001 cttttgcgag ccacagcagt gcaaggctta cgccgccgat cagggcagtt ggcacatcgg
     3061 ggtgcgcggc ggcatccgtc agcagcagcg ggagctggcc cacggcgatc aggatcgcgg
     3121 cgccggcggt gaagccggcg agcaccgggc ggctgagaaa tcccgacagg aagccgagcc
     3181 gcagcgcgcc acaggcgagc agtaccaggc cggtgagcag cgcgagctgc gtcgcgagcg
     3241 tgacgtagag caccgagccc gcgggcgcga gcgcggagag tgccgcggcg ctcatcagcg
     3301 aggtgatggc catcggtccc accgattgcg tcatgctgct gccgaagagc gcataggcca
     3361 ccggcggcag gatgctggca tacagtcccg ccgctggcgg caggcccgcc agcaacgcgt
     3421 aggccatgcc ctgcggaatc agcatcaacg cgacgatggc gccggcgccg agatcgcctg
     3481 ccagctgatc gcgccgatag ttccgcgccc actgcaacat ggccggtgca cacgctccgc
     3541 gaagctgaag tcagcgccag atcgggcgcc cacgctggcc gggcaggcgg ttcggcggca
     3601 tgcccagggc gcgcagcgtc tcgtcgacgc tgtcgtagta tgtcccgatc cggtaggtct
     3661 cgcgcgcttc gcctgccgtc gcgacgtcgc ggccgaggtg acgggcctgc gccacgcacc
     3721 actcgatctg ctgcaccgtg ctcatgcgct cgtggcgcgg cccccacaga ttgtcctcga
     3781 tgccgacgcg tacgtggtgg ccgagcgcga tcgcgatcgc gttggtgggg tgcatggtgc
     3841 gcatcagcga ctcgttggtg agcacggcgc cgtcgggcgt gcgtaccgca aactcgacca
     3901 ggtccgacgg gtgacgcgcg gcgaggccac cgccgatcgc cacgtaggtg aggttcagcg
     3961 ggcccatgta gacgcccgta cggatcaggg tctcgacggt ttccagctgg tgcacgtggc
     4021 cgagcatgaa gtaggcctgg acgccgttgt cgcgcagggc tttcagattg gtctcgtaga
     4081 aggtcgggcc ggcctcgagg tacatgtcgc ggtaggcggc ctgcatctcg ggcgccgcga
     4141 gcgaggtgcc ggcgacgtcg cgttcgctca tgagatcgac gatgttcatg ccgctggtac
     4201 cgatggcgat cgtcacctgg tccggcttgg gctcgagttg ggccagggcg tggcgcgagt
     4261 cgttgcccgg ccatttcgct tccttgcccg gctcgggagc gaaggagatc gagccaccga
     4321 cctgcaggat catcccgggt acggcctggc gcaggcgagc gatcagctcc tcgaacttgg
     4381 acagccgctt ggagcccgag ccgtcgtcct cgcgcacgtg gatgtgcagg atctgcgcgc
     4441 cggcgttgaa gcagtcgacg gctttctgca cctgctcgtc catcgtgacg ggaatgtcgt
     4501 cggagtcgcc cggcaggaat cccgggccgt agggtgcggc ggtgatcagc agcttttcct
     4561 gattctcggg gagcagtgaa tccttcaggt agtgcacggt gattacctcg ggtgagagcg
     4621 gatgttgatc ggggattcag aaggtgccct tgaccggacc ctcggtcgca agggcggcgg
     4681 cctcctcgat cagcatcggc gcgccgaggg ccaccgagtg gatgccgagc acggagacga
     4741 gttcgagcac ggtcatgacc tggtccctgg tggcgccgag ttccagtgcc ttgcggatgt
     4801 ggcggcgcac gcccggggcg tacaggtggg tgcaggaggc gtccaccgcg atcgcgatga
     4861 actcgatcgt caatggatcc agcgtgccgc ggcccatcgc gtgggcaccc atggccagga
     4921 atttctcgac ataggcggca tcgagttgtg ccggcacgtc ccacgccggg ttccagttgc
     4981 cggccgcacg catcctcgcg ctgacaggcg tggggctatc gtcatgcatc gttggttcct
     5041 cccgggtgcg ggtcgctgcg gagcgcgtcg acgggaagca agcatgacgg cgacacgtgc
     5101 tccggagttg acgtgccggg acgcggactt tacatttcgg gacacccgac gctgcagggc
     5161 cgatccatgc cgttcacgca cgtgcgcagc gcctcgctga ccggttacgc cgaggtcgcg
     5221 cgctccgttg gcctggatcc cttcgcccgg ctgcgggcgg ctggcctgga cgtccgctgc
     5281 ctggacgacc cggacctgaa gattcccgtg ctgcggctgc acgccctgct cgagtcctcg
     5341 gcgcatgcgg cgggtatcga agacttcggc ctgcgcatgg cgatgtcgcg gcgcctttcc
     5401 aacctcggct tgatctcgct ggcggcacgc gaagagccca ccgtgcgcga tgcactgcat
     5461 tgcctgcagc gcaccatgca cctgcacaac gaggcgatcc tgatcgacat cgaggaggcc
     5521 aacggcatcg tcgtgatccg
//
LOCUS       DAORUQ010000195         5448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18119_length_5448_cov_4.828111, whole genome shotgun sequence.
ACCESSION   DAORUQ010000195 DAORUQ010000000
VERSION     DAORUQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18119_length_5448_cov_4.828111"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PLW13_13635"
     CDS             complement(<1..1460)
                     /locus_tag="PLW13_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY71471.1"
                     /translation="MSSDDIPADWAPLLGEFRDRVEAGRAMGGEARLAKLRAAGRLNA
                     REQIAALCDPGSFVELGTLAGGQPHGEAPAAPADALVGGLARIDGRPVVVGAEDFSVQ
                     GGSIGFATHAKRLRYARIAQQERVPLIMLLDGAGERASNALSRHPYAPNDMQELARLS
                     GRVPSIAVVTGSSAGHGAITAMLSDLVIMLEKATIFAAGPSLVAAATGEVVSKEDLGG
                     ARIHATQSGVAHNVVPDEAAAFALIRSYLALLPSSAWAWPPARVSGDAGPRRLDAILH
                     LVPRDAQTPYDMRAVVRELLDDGEALELQPQFGRSLLTVLGRLGGETVAVLANQPMFL
                     AGSVDRDAADKAARFLQVADAFHVPVIFLADNPGILSGSKAERAGTLRAAARMFMAQS
                     QLRVPKLHVTLRKAYGFGSSIMAMNPFDGQTLTLAFPGISLGGLPAAGGARAAGLDST
                     AESRMAEAQAAGAWSAGDTLAYDEIIDPRELRNALLR"
     gene            complement(1461..3173)
                     /locus_tag="PLW13_13640"
     CDS             complement(1461..3173)
                     /locus_tag="PLW13_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQY71472.1"
                     /translation="MDPVKQKFDDAYALLTRAGAPYELVTETVGGQPLRAFRNTPRNM
                     RELFAPAYQHGDKEFVVFEGERWSFARLLGLADAIAHQLVHRSGVRKGDRVAIAMRNY
                     PEWMAAYIGITSAGATVVPLNSWGRAEELEFALSDAGARTAFCDRQRHDYLAPRAEAL
                     GLQLIVARPGDAPLPQASRSMEQYIAGAENAPLPAVDIEPEDIAVIAYTSGTTGKPKG
                     AVSTHRAVCQAIMCFECSGIATAMANPELFGAMMQKGFEPTSLLAVPLFHVSGCYAVF
                     LTSLRGGRRIVIMYKWDVEPALRLIQDERITVLTGAPSMIMELLESPLFGQYDTSSLF
                     NIGAGGAATPPKIGLLMQKRVHNSCPGTGWGMTETNALGSSFAGNAFHEHMGSSGFVQ
                     PLIELSFRDDDGNEVAPGEPGHIWVRSITLIKEYWNRPDANAKEFRDGWFNSGDIGFL
                     DADGYLYLTDRAKDMVIRGGENIFPLEVEKSLMDAPHVLETVAFGVPDDQWGEELAMV
                     VRLKQPGSMDENAVREFLGARLAAFKVPRYVSFTEQPLPRNATEKVLKKDVRHAFITS
                     HDLH"
     gene            3336..3785
                     /locus_tag="PLW13_13645"
     CDS             3336..3785
                     /locus_tag="PLW13_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71473.1"
                     /translation="MDIQEISDRLEINNLLIDYCSAVDAKSFADFDRIFTPDALIDYT
                     ALGGIRGSVAEIKEYLGRVMPHFGATQHLIANSRVWVEGDTARARTMCHNPMEIPLPG
                     SGTQIAFYGLWYVDRLVRTAQGWRIAERVEERSYDFNVPARFKSFSE"
     gene            3807..4679
                     /locus_tag="PLW13_13650"
     CDS             3807..4679
                     /locus_tag="PLW13_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY71474.1"
                     /translation="MQYTLSVGFCPADWYVPLARAAEAAGWDSIAVPDGLLYYEKTSA
                     PYPYSADGNRFWAADTPFLDPWVVIATMAGATERIRFYPSVLKLAVRDPLLVAKELSS
                     CAVLAGERVGLGVGLSPWPEDFEVLREEWAQRGPRSAEMIEIIRGLLRGGMYEYHGRF
                     YDFPRLQIAPVPRAPVPVYIGGLVEPVLKRAARLGDGYIGWENPRCSLEDLAQMIRRI
                     EAYRRDYGRSGAFEYKFMPYSTAPATRQRLAAAGVTDLICMPWMDTHGARAGLRERLD
                     AVARFGEEVIARSH"
     gene            4707..>5448
                     /locus_tag="PLW13_13655"
     CDS             4707..>5448
                     /locus_tag="PLW13_13655"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71475.1"
                     /translation="MRRFDGKVVMVTGAGSGIGRATAVRFASEGARVSVSDVNAEGLA
                     ATMGMLEGQGHHSQILDVSQAQACRDAVAATVAECGRLDVLCNIAGFAGAYHLHEITE
                     EFWQRMIAVNLSGVFFMCQAAMPHLLETRGNIVNMASSAAIVGQIYNSVYCASKGGVN
                     LLTKALAVEFATRGVRVNAVCPGAVNTPLSKNFRMPENPELAVFGRLMPLVQPSAEPE
                     EVAGAVAYLASDEARFITGDSLTIDGGQT"
BASE COUNT          841 a         1839 c         1900 g          868 t
ORIGIN      
        1 cgcagcaggg cgttgcgcag ctcgcgcgga tcgatgatct cgtcgtaggc cagcgtgtcg
       61 cctgcgctcc aggcgccggc cgcctgcgcc tcggccatgc gcgattccgc cgtgctgtcc
      121 agaccggcgg cacgggcacc accggcggca ggaagtccgc caagcgaaat gcccgggaat
      181 gcgagcgtca gcgtctgacc gtcgaaggga ttcatcgcca tgatcgagct gccgaagccg
      241 taggccttgc gcagcgtcac gtgcagcttc ggcacgcgca gctgtgactg cgccatgaac
      301 atgcgcgcgg cggcgcgcag cgtgccggcg cgctcggcct tgctgcccga gaggatgccg
      361 gggttgtcgg ccaggaagat caccggcacg tggaatgcat cggcgacctg cagaaagcgc
      421 gcggccttgt cggcggcgtc gcggtccacg gaaccggcga ggaacatcgg ctggttggcg
      481 agcaccgcaa cggtttcgcc gcctagcctc ccgagcacgg tcagcaggga gcgcccgaac
      541 tgcggctgca gctccagggc ctcgccgtca tcgagcagct cgcgcaccac ggcgcgcatg
      601 tcgtagggcg tctgcgcgtc gcgcggcacc aggtgcagga tcgcgtcgag ccggcgcggc
      661 ccggcatcgc ccgagacgcg ggcaggcggc caggcccagg cactcgaggg cagcagcgcg
      721 aggtaggagc ggatcagcgc gaaggcggcg gcttcgtcgg gcacgacgtt gtgcgcgaca
      781 ccgctctggg tggcgtggat ccgcgcgccg ccgagatcct ccttgctcac cacctcgccg
      841 gtggcggcgg cgaccagtga cgggccggcc gcgaagatcg tggccttctc gagcatgatg
      901 accaggtcgc tgagcatcgc cgtgatcgcg ccgtgtccgg ccgaggagcc cgtgaccacg
      961 gcgatgctgg ggacgcggcc cgagagtcgc gcgagctcct gcatgtcgtt gggcgcgtac
     1021 ggatggcgcg acagcgcatt gctcgcgcgc tcgccggcgc cgtcgagcag catgatcagc
     1081 ggcacgcgct cctgctgcgc gatgcgcgcg tagcgcaagc gtttggcgtg ggtcgcgaag
     1141 ccgatcgagc caccctgcac cgagaagtcc tcggcgccga ccaccacggg gcgcccgtcg
     1201 atgcgcgcca ggccgcccac gagcgcatcg gcgggggccg cgggcgcctc gccgtgcggc
     1261 tgaccgccgg ccagcgtgcc gagctccacg aaactgcccg ggtcgcacag cgcggcgatc
     1321 tgctcgcgcg cgttcaggcg cccggctgcg cgcagtttcg ccagtcgcgc ctcgccaccc
     1381 atcgcgcgcc ccgcctcgac gcggtcgcgg aactccccga gcagcggcgc ccagtccgcc
     1441 gggatgtcgt cgctgctcat tcagtgcaga tcgtgcgagg tgatgaaggc gtggcgcacg
     1501 tccttcttca ggactttctc ggtcgcgttg cgcggcaggg gctgctcggt gaagctgacg
     1561 tagcggggca ccttgaatgc ggccagtcgc gcgccaagga actcgcgcac ggcgttctcg
     1621 tccatgctgc cgggttgctt caggcgcacg accatcgcga gctcctcgcc ccactggtcg
     1681 tcgggcacgc cgaaggcgac cgtttcgagc acgtgcggcg catccatgag cgacttctcc
     1741 acttcgagcg ggaagatgtt ctcgccgccg cggatcacca tgtccttggc gcggtcggtt
     1801 aggtagaggt agccgtcggc atcgagaaaa ccgatgtcgc cgctgttgaa ccagccgtcg
     1861 cggaactcct tggcgttggc gtccgggcgg ttccagtatt ccttgatcag cgtgatgctg
     1921 cgcacccaga tgtgacccgg ttcgccgggc gccacctcgt tgccatcgtc gtcacggaaa
     1981 ctgagctcta tgagcggttg cacgaagccc gagctgccca tgtgctcgtg gaaggcgtta
     2041 cccgcgaagg agctgccgag cgcattggtc tcggtcatgc cccagccggt gcccgggcac
     2101 gagttgtgta cccgcttctg catgagcaga ccgatcttcg ggggcgtggc cgccccgccg
     2161 gccccgatat tgaacaggct gctggtatcg tactgcccga acagcggcga ctcgagcagc
     2221 tccatgatca tcgagggggc gccggtcagt accgtgatgc gctcgtcctg gatcaggcgc
     2281 agcgccggtt ccacgtccca cttgtacatg atcacgatgc gccttccccc gcgcagcgag
     2341 gtcaggaaca ccgcgtagca gccgctcacg tggaacaggg gcaccgcgag caggctggtg
     2401 ggctcgaagc ccttctgcat catcgcgccg aacagctccg ggttggccat cgcggtggcg
     2461 atgcccgagc actcgaagca catgatcgcc tggcacaccg cgcggtgcgt cgagaccgca
     2521 cccttgggct tgcccgtggt gcccgaggtg taggcgatga ccgcgatgtc ctcgggctcg
     2581 atatccaccg cgggtagcgg cgcattttcg gcaccggcga tgtactgctc catgctgcgc
     2641 gatgcctggg gcagcggcgc gtcgccggga cgcgcgacga tcagctgcag tcccagcgcc
     2701 tcggcgcgcg gggccaggta gtcgtggcgc tggcggtcgc agaaagccgt cctggcgccc
     2761 gcgtcgctca gcgcgaactc gagttcctcg gcgcggcccc agctgttcag cggcaccacg
     2821 gtcgcccccg cgctcgtgat accgatgtag gccgccatcc actcggggta gttgcgcatc
     2881 gcgatcgcga cacggtcgcc cttgcgcacg ccgctgcgat ggaccagctg gtgcgcgatc
     2941 gcatcggcca gtccgagcag ccgcgcaaag ctccagcgct cgccctcgaa gaccacgaat
     3001 tccttgtcgc cgtgctggta ggccggtgcg aacagctcgc gcatgttgcg cggcgtgttg
     3061 cggaacgcgc gcaacggctg cccgccgacg gtctcggtga ccagttcgta gggcgcgccg
     3121 gcccgggtga gcagggcgta ggcgtcatcg aatttctgct tgacagggtc catgtggtct
     3181 ccgggaaatc gcgggaggcc gcattataag gagcagtgcc cgcgccacaa agccgcgcat
     3241 gacgcatttg taaagtttcg cgggaccatc tgctatagtc gggcttgccg aggcgtgcca
     3301 tgatccggca aacacaacgg aggcccccag tggcgatgga tatccaggaa atttccgacc
     3361 ggctcgagat caacaacctg ttgatcgact actgcagtgc agtcgatgcc aagagcttcg
     3421 ccgacttcga ccggatcttc acgcccgacg cgctgatcga ctacaccgcg ctgggcggca
     3481 ttcgcggcag cgttgccgag atcaaggagt acctggggcg cgtgatgccg catttcggcg
     3541 ccacgcagca cctgatcgcc aacagccgcg tgtgggtgga gggcgacacg gcgcgcgcac
     3601 gcacgatgtg ccacaacccc atggagatcc cgctgcccgg gagcggcacc cagatcgcgt
     3661 tctatggcct gtggtacgtc gaccgcctgg tgcgcacggc gcagggatgg cgcatcgccg
     3721 agcgcgtcga ggagcgcagc tacgatttca acgtgccagc caggttcaag tcgttcagcg
     3781 agtaggccgc gcgcaccgga ggcgatatgc agtacacgct ctcggtcggc ttctgtcccg
     3841 ccgactggta cgtcccgctg gcgcgtgccg ccgaggccgc cggctgggac tcgatcgcgg
     3901 tgcccgacgg gctgctctac tacgagaaga cctccgcgcc gtatccgtac agcgccgatg
     3961 gcaaccggtt ctgggccgcc gacacgccgt ttctcgaccc ctgggtggtc atcgcgacga
     4021 tggccggtgc caccgagcgc atccggttct atccctcggt gctgaagctg gcggtgcgcg
     4081 atccgctgct ggtcgccaag gaactgagct cctgcgcggt gctggcgggc gagcgcgtcg
     4141 ggctcggcgt gggactcagt ccgtggccgg aggacttcga ggtgctgcgc gaggagtggg
     4201 cacagcgcgg gccgcgctcg gccgagatga tcgagatcat ccgcggcctg ctgcggggcg
     4261 ggatgtacga ataccacggc cgtttctacg acttcccgcg gctgcagatc gcgccggtgc
     4321 cacgcgcgcc ggtaccggtc tacatcggcg gcctcgtcga gccggtgctc aagcgcgccg
     4381 cgcgcctcgg ggacggctac atcggctggg agaatccgcg ctgctcgctc gaggacctgg
     4441 cccagatgat ccggcgcatc gaggcttacc gccgcgacta tggtcgcagc ggtgccttcg
     4501 agtacaagtt catgccctac agcaccgcgc ccgcgacccg gcagcgtctc gccgccgccg
     4561 gcgtgacgga cctgatctgc atgccatgga tggacacgca cggggctagg gcgggattgc
     4621 gcgagcggct ggatgcggtc gcgcgtttcg gcgaggaagt cattgcacgg tcacattgac
     4681 caggacccca gaggagaact gacaggatgc ggcgtttcga cggcaaggta gtgatggtga
     4741 cgggtgccgg ctcgggaata gggcgtgcca cggcggtgcg cttcgcgtcc gagggcgcgc
     4801 gcgtctcggt gagcgacgtg aatgccgagg gactcgccgc gaccatgggc atgctcgagg
     4861 gccaggggca tcactcgcag atcctcgacg tctcgcaggc gcaggcctgc cgcgacgcgg
     4921 tggcggccac ggtcgccgag tgcggccgtc tcgacgtgct gtgcaacatt gccggtttcg
     4981 ccggcgccta tcatctgcac gagatcaccg aggaattctg gcagcgcatg atcgcggtca
     5041 acctctcggg cgtgttcttc atgtgccagg ccgcgatgcc acatctgctc gagacccggg
     5101 gcaatatcgt gaacatggca tccagcgcgg ccatcgtcgg ccagatctac aacagcgtct
     5161 actgcgccag caagggcggc gtgaacctgc tgaccaaggc gctggccgtc gagttcgcca
     5221 cccgcggcgt gcgcgtgaac gcggtgtgtc cgggcgcggt caacacgccg ctgtcgaaga
     5281 acttccgcat gcccgaaaac cccgagctgg cggtgttcgg gcgcctgatg ccactggtac
     5341 agccctcggc cgagccggag gaagtcgccg gcgcggtcgc ctatctcgcc agcgacgaag
     5401 cacgcttcat caccggcgat tcgctcacca tcgacggcgg ccagaccg
//
LOCUS       DAORUQ010000196         5442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18148_length_5442_cov_4.991647, whole genome shotgun sequence.
ACCESSION   DAORUQ010000196 DAORUQ010000000
VERSION     DAORUQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18148_length_5442_cov_4.991647"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1144
                     /locus_tag="PLW13_13660"
     CDS             338..1144
                     /locus_tag="PLW13_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQY71476.1"
                     /translation="MEIWAVANQKGGVGKTTTVVSCAGLLAESGKRVLMVDLDPQGSL
                     TCYFRHEPATLAHGTYELFREHRLPPPLVLNAMFLPTPHPGLKLFPAAPGLATVERQF
                     RAEEGVGLRLARALASLRGSFDYVLIDTPPVLGVLMINALAASQRLLLPVQTDYLAIK
                     GLERMAHTLAMVVRARGREVAWTVVPTMFDAGSEAAASGLRALLRAFTDRVGDTVIPL
                     DNSFREASLHGALPSQFAPGSRGVRSYRALLASLGVLEVEEPQRRLGPAA"
     gene            1192..1614
                     /locus_tag="PLW13_13665"
     CDS             1192..1614
                     /locus_tag="PLW13_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71477.1"
                     /translation="MEKEAGRRQFFDGIAAAGGVPMMTLDSLTLLVAPLGAVLALLVA
                     WAGLRHVRRVLRARCAPLEEALRVYSGANVAMGKHVTALETELREMRQRLAAMEGAGL
                     RARLREPESAQPRPEEGCVAEQRLSLLIRSRLGGLRPG"
     gene            complement(1585..1953)
                     /locus_tag="PLW13_13670"
     CDS             complement(1585..1953)
                     /locus_tag="PLW13_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016498575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQY71478.1"
                     /translation="MDGPQRPARRAIALEYDGARAPRISALGEDAVAREIIALAREHG
                     VPLLENAELAGLLAQLDLGDEIPETLYRCVAQIIAFAYRLRGRFPRDWTAPQGGAEPG
                     ATPPSAWAAPPSAGPQPTEP"
     gene            complement(1953..4022)
                     /locus_tag="PLW13_13675"
     CDS             complement(1953..4022)
                     /locus_tag="PLW13_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQY71479.1"
                     /translation="MTPGSVLPRALQAQLGLPPPPAGVASPPWPIDALEALVIAARPL
                     ATPSTGWQLEIDIGGERVQMNSTTLLAAGTRLLLRALSPQRVRVEQVLSEPAQLQPLR
                     NALRSDLPAQLPLREAIGGLAMLAADPALPEPLRARIGQLLAQLPDPAQLQLAAGLRA
                     AVLNSGSFLEPRLRELTMAATVASAPPAAARTASAAAAPSPATAIGSTPSARLAPAAV
                     AAPATGPRAALAALLRLLLPARAGPAPANAPVAAGPAGASPPSALTPALTVPLYDAQG
                     ALQRAGAALPGAAAPAPDSPVATVPLTRAPAALAATAGALPGGMNGADTITAPLAPPG
                     LPGATATRATEGAAPGLAPAPVPPGASPAPRSSAPTAVTEVPANATAGVATRLTVPDA
                     SSPGPAARPAGLSAAPAAGTGDTPRPGLGAAPAAFPGAGTDTAARGVAPAGVAADLKG
                     QLFVLLEAASGWLRAHPGSSRSPPGASAPGLPLYTARGLVAGDSAAVEAVAPALPATA
                     QLAARAAQQLPGPDPSRPRDPEDLVETLLRYVVGALARTRVHQLGGHPESRRQGDSGA
                     LQAWSVEIPIAQGTRMDVLEMQVEEHGRHEGPRGGAERVWQVLMRLDLEQVGPLHALL
                     RLSGTRLSTTLWIENPGALAPARDTLAELGDLLRAEGVDVTRLECLPGSPPPRARGHD
                     NLLDVST"
     gene            4166..4813
                     /gene="ccmA"
                     /locus_tag="PLW13_13680"
     CDS             4166..4813
                     /gene="ccmA"
                     /locus_tag="PLW13_13680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HQY71480.1"
                     /translation="MAQQASVPLLEVRALRCERDERLLFEDVRFELRAGEVMQVAGPN
                     GCGKTTMLRTIAGLSQRCTGRVMWRGEDIGRARYDYLRESLYLGHEAAINPALTPLES
                     LRWHASLWGGVRPLAIEEALARTGLAGYLHTPAQRLSAGQKRRIALARLLLSPATLWI
                     LDEPFTAIDLAGVGELESLIAAHAAAGGAVLLTTHHRLGLANGLRRLMLGQQMVD"
     gene            4813..>5442
                     /gene="ccmB"
                     /locus_tag="PLW13_13685"
     CDS             4813..>5442
                     /gene="ccmB"
                     /locus_tag="PLW13_13685"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQY71481.1"
                     /translation="MDIQGAFAETLRRDLLIALRRRGDIANPLVFFVIVIALFPLGIG
                     PTPEDLARIAPGVLWVVALLASLLSTDMVFRADHDDGSLEQLLRSPDPLYLLAIAKIA
                     VHWLVTGLPLTLLSPLMGGMLYLPAAAAPALLLSLLLGTLTLSLVGGIGAALTVGLRK
                     GGILLSLLILPFYIPVLVFGTAAVDAAALGADYLPHLAILGAFALVALTL"
BASE COUNT          656 a         1898 c         2108 g          780 t
ORIGIN      
        1 gatgctctcg cgcggtgcgc ggttgcggcc cgagttgccg cgcacgccac tgccggtgcc
       61 ggtctcgacg atacccgtgc ccgcggcgcc ggccgcgacg aacgaggcgg gaacgcccgc
      121 agccgatgcg gcggcagcgt cggaccggcc tgctgcagca gtcacgccgc aggcaccggg
      181 gccggcaggc gtggttcgcg tcgagctcga aggtgggggt atcctgtaca ccaacgatcc
      241 ggcgcggggt ggggcacagg gcgcagcggc tgccgagggc gccactgcgc cgctcgaggc
      301 agcgcaaccg ccgtagtcat cgatcgggga gccgggtgtg gaaatctggg cagtcgcgaa
      361 ccagaagggc ggagtgggca agaccaccac ggtggtgagt tgtgccgggc tgctggccga
      421 gtcgggcaag cgcgtgctga tggtcgacct cgatccgcag ggatcgctga cctgctattt
      481 ccgccatgag ccggcgaccc tggcccacgg cacctacgaa ttgttccgcg aacaccgcct
      541 gccgccgccc ctggtgctca acgcgatgtt cctgcccacg ccgcacccgg ggctgaagct
      601 gtttcccgcc gcgccggggc tcgcgacagt cgagcggcag ttccgcgccg aggagggcgt
      661 tggcctgcgc ctggcacgcg cgctggcctc gctgcgcggc agtttcgatt acgtcctgat
      721 cgacaccccg ccggtgctcg gcgtgctgat gatcaacgcg ctggcggcga gccagcgcct
      781 gctgctgccg gtgcagaccg attacctggc gatcaagggg ctggagcgca tggcgcacac
      841 gctggcgatg gtggtgcgcg cccgcggacg cgaggtcgcc tggacagtgg tgccgaccat
      901 gttcgatgcc ggcagcgagg cggcggccag tggcctgcgg gccctgctgc gcgccttcac
      961 ggaccgggtc ggcgataccg tgataccgct cgacaacagc tttcgcgagg ccagcctgca
     1021 cggcgcgctg ccctcgcagt tcgcgcccgg gagccgtggc gtgcgctcct accgcgcact
     1081 gctcgcgagc ctgggggtgc tcgaggtcga ggagccgcag cgccgcctgg gtcccgccgc
     1141 ctgaggccgt gaccggcaag ctcgagcaag cgtggcacgc ttggtgctag gttggagaaa
     1201 gaagccggca ggcgacaatt tttcgacggt attgccgcgg cggggggcgt gcccatgatg
     1261 acactcgatt cgctgacgtt gctggtggcg cccctgggcg ccgtgctggc gctgctggtg
     1321 gcgtgggcgg gcttgcgcca cgtccgccgg gtgctgcgcg cgcgctgcgc gccgctcgag
     1381 gaggcgctgc gcgtctacag cggcgccaat gtcgcgatgg gcaaacacgt cacggccctg
     1441 gagacggaat tgcgcgagat gcgccagcgg ctcgcggcaa tggaaggggc cggcctgcgc
     1501 gcacggctgc gcgagcccga gtcggcccag ccgcggccgg aggaagggtg tgtcgccgag
     1561 cagcgcctgt cgcttctgat ccgctcacgg ctcggtgggc tgcggcccgg ctgagggcgg
     1621 cgcggcccag gctgaaggtg gcgttgcgcc cggttcggcg ccgccctgcg gcgccgtcca
     1681 gtcgcgcggg aagcgcccgc gcagccggta ggcgaacgcg atgatctgcg ccacgcagcg
     1741 gtagagggtc tcgggtatct cgtcgccgag atcgagttgc gccagcaacc cggccaactc
     1801 ggcattctcc agcagcggta cgccgtgttc gcgcgccagc gcgatgatct cgcgcgccac
     1861 cgcgtcctct cccagcgccg atatccgcgg cgcgcgcgcg ccgtcgtatt cgagcgcgat
     1921 cgcgcggcgc gcgggtcgtt gcgggccgtc catcaggtgc tcacgtccag caggttgtcg
     1981 tggccgcgtg cccgcggggg cggcgatccg ggcaggcact cgagccgggt gacgtcgacg
     2041 ccctcggcgc gcagcaaatc gccgagttcg gcgagcgtat cgcgcgccgg ggccagcgcg
     2101 ccggggttct cgatccacag cgtggtggac agtcgcgtgc cgctcagtcg cagcagggcg
     2161 tgcagcggac cgacctgttc gaggtccagg cgcatcagca cctgccacac gcgctcggcg
     2221 ccaccgcgcg gcccctcgtg ccggccgtgc tcctcgacct gcatctccag tacgtccatc
     2281 cgcgtgccct gcgcgatcgg aatctcgacg ctccaggcct ggagcgcgcc gctgtcgccc
     2341 tggcggcggc tttcgggatg cccccccaac tggtgcacgc gggtgcgggc gagtgcgccc
     2401 accacgtagc gcagcagggt ttccacgaga tcttcggggt cgcgcggtcg cgatggatcg
     2461 gggccaggga gctgttgtgc cgctcgggcc gccagctggg ccgtggcggg gagtgccggg
     2521 gcgacggctt ccacggcggc gctatcgccg gcaaccaggc cgcgcgccgt gtacagcggc
     2581 aggccgggtg cgctcgctcc gggcggcgag cggctcgagc ccgggtgcgc gcgcagccaa
     2641 cccgacgccg cttccagcag cacgaacagc tggcccttca gatctgcggc aacgcccgcg
     2701 ggcgcgacgc cgcgcgccgc tgtgtcggtg ccggccccgg ggaaggcagc gggcgccgcg
     2761 cccagcccgg gccgcggtgt gtcgccggtg ccggcggcgg gcgccgcgga taggcccgcc
     2821 ggtctcgccg cggggccggg cgacgaggca tcgggcacgg tgagtcgggt cgcaacgcca
     2881 gccgtggcgt tggctggcac ttcggtcacg gccgttggcg cgctggagcg cggcgcgggg
     2941 gacgcgcccg gtgggactgg cgctggcgcg aggccgggcg ccgctccttc ggtggcgcgc
     3001 gtcgcggtcg cgccgggcag cccgggcggt gcaaggggcg ccgtgattgt gtccgcgccg
     3061 ttcatgccgc cgggcagcgc gcccgcggtg gcggccagcg cggccggtgc ccgggtgagc
     3121 ggcacggtgg ctaccgggct gtccggtgcg ggtgccgctg caccgggcag agcggcgccc
     3181 gcccgctgca gcgcaccctg cgcatcgtag agcggcaccg tcagcgccgg ggtgagggcc
     3241 gacggcggcg atgcgcccgc cgggcccgcg gcgacgggag cattcgcggg agcggggccg
     3301 gcgcgcgccg ggagcagcag gcgcagcagt gcggccagcg cggcgcgcgg accggtggcg
     3361 ggcgccgcga cggctgcggg tgcgagccgc gcggatgggg tggagccgat ggcggtggcg
     3421 ggcgagggcg cggcagcagc gctggcggtg cgtgcggcgg cgggcggcgc agacgcgacg
     3481 gtggcggcca tcgtcagttc gcgcaggcgt ggttcgagga agctgccgct gttcagcacc
     3541 gccgcgcgca ggccggcggc cagctgcaac tgtgcgggat cggggagctg cgcgagcagc
     3601 tgcccgatgc gcgcgcgcaa cggttcgggg agcgcgggat cggcagccag catcgccagg
     3661 cccccgatgg cctctcgcag cgggagctgg gccgggaggt cgctacgcag ggcgttgcgc
     3721 agcggctgca gttgcgccgg ctcgctcagc acctgttcga cgcgcacgcg ttgcggcgac
     3781 agcgcgcgca gcagcaggcg cgtacccgcg gccagcagtg tggtgctgtt catctgcacg
     3841 cgctccccgc cgatgtcgat ttcgagctgc cagccagtgg acggtgtcgc cagcgggcgg
     3901 gcggcgatca ccagcgcctc gagcgcgtcg atcggccacg gcggcgacgc gacgcccgcg
     3961 ggcggcgggg gcaggccgag ctgggcctgc agtgcgcgcg gcagcacgct gcccggggtc
     4021 atgtccatgc tcgttgccag cggggtgagg gcttcacata taatcgcgcg gttcgatgcg
     4081 attatagcgg cggcgctcgc cggaagttga gtccgtaggg caaccccggc gcacaccgtc
     4141 ccacaacccc ccggaagccc ccgcgatggc gcaacaggcc tcggttccgt tgctcgaggt
     4201 tcgcgcgctg cgctgcgaac gcgacgagcg cctgctgttc gaggacgtgc ggttcgagct
     4261 gcgcgcgggc gaggtcatgc aggtcgcggg acccaacggc tgcggcaaga ccacgatgct
     4321 gcgcaccatc gcgggcctct cgcagcgctg caccggccgc gtcatgtggc gtggcgagga
     4381 catcggccgc gcgcgctacg attacctgcg cgagtcgctg tacctcggtc acgaagcggc
     4441 gatcaatccg gcgctgacgc cgctcgagag cctgcgctgg cacgcctcgc tgtggggcgg
     4501 ggtgcgcccg ctcgccatcg aggaggccct ggcccgtacc ggtctggccg gctatctgca
     4561 cacgcccgcg cagcgacttt ccgccggtca gaagcggcgc attgcgctgg cgcggctcct
     4621 gctcagtccc gcgacgctgt ggatcctcga cgagcccttc accgcgatcg acctcgccgg
     4681 cgtgggcgaa ctcgaatcgc tgatcgccgc gcatgccgcg gcaggtggcg cggtgctgct
     4741 caccacgcat caccgcctcg ggctcgcgaa cgggctgcgc cggctcatgc tcggccagca
     4801 gatggtggat tgatggacat ccaaggcgca tttgccgaga ccctgcgcag ggacctgctg
     4861 atcgcgctgc gccgccgtgg cgacatcgcc aacccgctgg tgttcttcgt gatcgtgatc
     4921 gcgctgttcc cgctcggcat cgggccgacg ccggaggatc tcgcgcgcat cgcacccggc
     4981 gtgctgtggg tggtcgcgtt gctggcctcg ctgctgtcga cggacatggt gtttcgcgcc
     5041 gatcacgacg acggctcgct ggagcaactg ctgcgcagcc ccgatccgct gtacctgctg
     5101 gcgatcgcga agatcgcggt gcactggctc gtcaccgggc tgccgctcac gctgctctcg
     5161 ccgctgatgg gggggatgct ctacctcccg gcggcggcgg ctccggcgct gctgctgtcg
     5221 ttgctgctgg gcacgctgac actcagcctg gtggggggca tcggggcggc gctcacggtg
     5281 ggcttgcgca agggcggcat cctgctgtcg ctgctgatcc tgccgttcta catcccggtg
     5341 ctggtgttcg gcacggccgc ggtcgatgcc gcggcgctgg gtgccgacta cctgccgcac
     5401 ctggcgatcc tcggcgcctt cgcgctggtc gcgctcacgc tg
//
LOCUS       DAORUQ010000197         5411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18333_length_5411_cov_7.031367, whole genome shotgun sequence.
ACCESSION   DAORUQ010000197 DAORUQ010000000
VERSION     DAORUQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18333_length_5411_cov_7.031367"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..2023
                     /locus_tag="PLW13_13690"
     CDS             488..2023
                     /locus_tag="PLW13_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71482.1"
                     /translation="MNDIPGNTPILVGAGQYVERGASAESPSSLAARAGAAALADAGG
                     TALARAIDTIAVVRFFADSSPLWTCAFGRSNNPPQSIARAIGANPRQRIYTEVGGNQP
                     QSRLTEFARDIARGERDLVLFAGAEAIRNQRQAERQGATLDWNEEFDVPLDDRGLGAD
                     LFTSQEIHNGLIMPVYFYTLVEQARASAAGRSTAQQRAHSAALMASFSAVAAANPYAQ
                     FAGAQSAQEILAAAPMTHLYTRRMVAQDGVNQAAALLMTSVAKARELGIPRDRWVFLH
                     GMAEGAEWMLSERPDVGEAPVAGAVLARALAMAGRSVADIGPIDVYSCFPCAISVIAQ
                     HLGLPDDGSRALTLTGGLPYFGGPGNNYSMHGIAEMVWRLRREPEAFGLVTANGGMLS
                     KHAAGIYSCRPSIVDWSRADTAVDGQGFARRSLTTLADAGRIVTWTVNWSGNEPVQAV
                     VIGETDAGERFVACSAPGDAATVHAMLGRDRTGHGVRVRPGNGETRHFTLAQDARSTT
                     VER"
     gene            2029..3459
                     /locus_tag="PLW13_13695"
     CDS             2029..3459
                     /locus_tag="PLW13_13695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY71483.1"
                     /translation="MQRVSSADAGMLHAETPEMPMHTMGVLLLEKPAHSLYDRLRRSL
                     QKRLHLIEPFRRRLVTGPLQIGDPHWLEDPDFDLDNHLLRTALPAPGGTRELEDYAGD
                     LAGRLLDRARPLWEMHLVEGLRGGRVALIVKVHHAAMDGGRLVALMGALLDPSPRGRR
                     IPPPDHARIPEQEPSLAWMALDASLTVAAKPLNVLRTVTEIGGVLLRNRGGDAGDTGP
                     QEHRPKVFDAPATPFNGRLSAQRVVAMADVAFEDVKLIKDAFGTTVNDVVLAASCAGL
                     RAWLLAHDAPPAQPLIATVPVSVRGEGAGEAESGNRVSMILAELPVQDDDSVGRLRAI
                     NAATSRAKQHHGSSKGDVFRQTSELLLNVSTPWMLQHIMALYSGSDLPERLPLPWNLV
                     ISNLPGPREPLYCAGARVLRVYPFGPLQLGSGLNITVMSAIDRLCVGALACKRRMPDV
                     ALVTQALVAEIALLKQLARKARRRGS"
     gene            complement(3480..4112)
                     /locus_tag="PLW13_13700"
     CDS             complement(3480..4112)
                     /locus_tag="PLW13_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71484.1"
                     /translation="MQPPSDTMPETIVAQSSEHLTIALERGVYWTPPPVPARAPARRQ
                     RLGWACARVAFHAYVGFALFSGVGGFLTAPLMTHLFFDDWRFWRYWRRGAALFPHGWR
                     LLLLVLRGEGTFMLGVPLDSPPLSRPLRGAAQLSGAWPHGDSCGSCRRCCQVKSLCCP
                     ILEPDTGRCGGYDSFYWRYFNCGRYPSSQSEIDYYGCPKWVISGQEAGAG"
     gene            4306..4986
                     /locus_tag="PLW13_13705"
     CDS             4306..4986
                     /locus_tag="PLW13_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71485.1"
                     /translation="MPATLASAEPRRARGRPRRLTPEAVLDAALALFEDEPAAEFTLG
                     RLARRLGVPITSLYTCFPNRDALLDAAADRVFARFDWKPEPRSPWTAVLLSWMHAVQR
                     HFERYPIAQQLLTWDRRISPAWLRVLAPIITLLHAQGLRGRALVQTFSWLNSGTIGLI
                     MSHAYNARHQARPALGAAELAADGEGLGAQLEFWSHVNELDHEQSLDAGFRVLVAGVA
                     AALPAQKR"
     gene            5017..>5411
                     /locus_tag="PLW13_13710"
     CDS             5017..>5411
                     /locus_tag="PLW13_13710"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71486.1"
                     /translation="MSDMLIKGGTVIDGTGAPARRADVRVRNGVIRRIAPDLAPESGE
                     RVFDASGCQVTPGFIESHTHFDGTMWWQPDMDPLPGYGVTTTIMGNCGFSAAPVHEDP
                     KVRGEMVYIFSFFEDIPEKPFLKQLPWDWR"
BASE COUNT          866 a         1918 c         1857 g          770 t
ORIGIN      
        1 cccctgcagg ctacctgtcg ccgtcctggc gacagagtgt ccgccgcacc gatggctgct
       61 cgagcccctc gccgtggtgg cccccccgaa aaggcgtgca tacccggatt tgctcgatgc
      121 cgcacgcgct caaccgagcg gctgtgctgc ccgctgttgg cttgcggggc cttgcgccgc
      181 aggacgcggc gcggagctac agggacgtac tcgtgcgtgc cccgcaggac aatagcgggc
      241 agcacagccg gcgacgcacc aggccgctcg ctcgcaacgt catggctgtt gcggcaccct
      301 cttcagcccg ataaaggttc ggcgttgccg aaccctccga ctatcagcca tatacccgtc
      361 ggcataaatg ccgccccaca cccgacccac acggggcatc gccggcgcgc cccagcgcct
      421 cacgtttggg tacacttgcc gccaccgtga acggcgcccg cacggcgccc ggcaaggagc
      481 atccagcgtg aacgatatcc cgggtaacac ccccatcctg gtcggcgccg gccagtacgt
      541 cgagcgcggc gccagcgccg agtcgccatc gagcctggcg gcacgcgccg gcgccgcggc
      601 gctggccgac gccggcggca ctgcgctcgc ccgcgccatc gacaccatcg ccgtggtgcg
      661 cttctttgcc gactcctcac cgttgtggac ctgcgccttc gggcgcagca acaacccgcc
      721 gcagtcgatc gcgcgcgcca tcggcgcgaa tccgcgccag cgcatctaca ccgaggtcgg
      781 cggcaaccag ccgcaatcgc ggctgaccga gttcgcacgc gacatcgcgc gcggcgagcg
      841 cgacctggtg ctgttcgccg gcgccgaggc gatccgcaat cagcgccagg ccgaacgcca
      901 gggcgcgacg ctcgactgga acgaggaatt cgacgtgccg ctggatgacc gcggcctcgg
      961 cgcggacctg ttcacctcgc aggagatcca caacggcctg atcatgccgg tgtatttcta
     1021 cacgctcgtc gagcaggcgc gcgcgagcgc ggcgggccgc tcgacggcac agcagcgcgc
     1081 gcacagcgcc gcgctgatgg cttcgttcag cgcggtcgcg gcggccaacc cctacgccca
     1141 gttcgccggt gcgcaatcgg cgcaggagat cctcgccgcc gcgccgatga cgcacctcta
     1201 cacccggcgc atggtggcgc aggacggcgt gaaccaggcc gcggcgttgc tgatgaccag
     1261 tgtggccaaa gcacgcgagc tcggcatacc gcgtgatcgc tgggtgttcc tccacggcat
     1321 ggccgagggc gcggaatgga tgctgagcga gcgccccgac gtcggcgagg ccccggttgc
     1381 cggcgcggtg ctggcgcgcg ccctcgcgat ggccgggcgc agcgtggccg acatcggccc
     1441 gatcgacgtc tacagctgct tcccgtgcgc catcagcgtc attgcgcaac acctgggcct
     1501 gcccgacgac ggcagccgcg cgctgacgct gaccggaggc ttgccgtact tcggcggtcc
     1561 gggcaacaac tactccatgc acggcatcgc cgagatggtg tggcgactgc gccgcgagcc
     1621 cgaggcattc ggcctggtga cggccaacgg cggcatgctg tcgaagcatg ccgccggcat
     1681 ctactcgtgc cggcccagca tcgtggactg gagccgcgcc gacaccgcgg tcgacgggca
     1741 ggggtttgcg cgccgctcgc tcaccacgct ggcggatgca gggcgcatcg tgacctggac
     1801 cgtgaactgg tccggcaacg agccggtgca ggcggtggtg atcggggaga cggatgccgg
     1861 ggaacgcttc gtggcctgct ccgcgcccgg cgatgcggcc accgtgcacg cgatgctcgg
     1921 gcgcgaccgc accggccatg gtgtgcgggt gcgcccgggc aacggcgaga cccggcattt
     1981 cacgctggcg caggatgcgc gaagcaccac tgtcgagagg taaggaccat gcaacgtgtc
     2041 tccagtgcgg atgccgggat gctgcacgcc gaaaccccgg agatgcccat gcacacgatg
     2101 ggcgtactgc tgctcgaaaa gcccgcgcat tcgctctacg accggctgcg ccgctccctg
     2161 cagaaacgcc tgcacctgat cgagccgttc cgccgccgac tcgtgacagg ccccctgcag
     2221 atcggcgatc cgcactggct cgaagatccc gacttcgacc tcgacaacca cctgttgcgc
     2281 acggcactgc cggcaccggg cggcacgcgc gagctcgagg actatgccgg cgacctggcc
     2341 gggcgcctgc tcgatcgcgc gcggccactg tgggagatgc acctggtcga ggggctgcgc
     2401 ggtggccggg tcgcgctgat cgtgaaggtg catcacgccg cgatggacgg cggcaggctg
     2461 gtggcgctga tgggcgcgct gctcgacccc tcgccgcgcg ggcgcaggat tcccccgccg
     2521 gaccacgccc ggataccgga acaggaaccc tcgctggcat ggatggcgct cgacgcctcg
     2581 ctcaccgtgg cggccaagcc gctcaacgtg ctgcgcaccg tcaccgagat cggcggcgta
     2641 ctgctgcgca atcgcggcgg cgatgcaggg gataccgggc cgcaggagca ccggcccaag
     2701 gtgttcgacg cgccggcgac cccgttcaat ggccgcttgt ccgcccagcg cgtggtggcc
     2761 atggccgacg tggcgttcga ggacgtgaag ctgatcaagg atgccttcgg caccacggtc
     2821 aatgacgtcg tgctggccgc cagctgcgcg ggactgcgcg cctggctgct cgcccatgac
     2881 gcgccgcccg cgcagcccct gatcgcgacc gtgccggtct ccgtgcgcgg cgagggcgct
     2941 ggcgaggccg aatccgggaa tcgcgtgtcg atgatcctgg ccgagttgcc ggtgcaggac
     3001 gatgactccg tggggcgcct gcgggccatc aatgccgcga cctcgcgagc caagcagcac
     3061 catggcagca gcaagggcga tgtgttccgc cagacctcgg agctgctcct caacgtgtcg
     3121 acgccatgga tgctccagca catcatggcg ctgtattccg gcagcgacct gcccgagcgg
     3181 ctgccactgc cctggaacct cgtgatctcg aacctgccgg ggccgcgcga accgctctac
     3241 tgcgccggcg ccagggtgct gcgggtatat cccttcgggc cgctgcaact cggctcgggc
     3301 ctgaacatca cggtgatgag cgcgatcgat cgcttgtgcg tgggggcgct cgcctgcaag
     3361 cgccggatgc cggacgtggc gctggtcacg caggccctcg tggccgaaat cgcgttgttg
     3421 aagcaactcg cgcgcaaggc ccggcgccgc ggttcctgag cggacacggc gcttgcgcgt
     3481 cagcctgccc cggcttcctg cccgctgatc acccacttgg gacagccgta atagtcgatc
     3541 tcggactgtg acgaggggta gcggccgcag ttgaaatagc gccagtagaa ggagtcgtag
     3601 cccccacagc ggccggtgtc cggttctagg atcgggcagc agagcgactt gacctggcag
     3661 cagcgccggc aactgccgca ggagtcaccg tggggccatg cccccgacag ctgcgcggcg
     3721 ccgcgcagcg gcctggacag cggcgggcta tcgagcggta ccccgagcat gaacgtgccc
     3781 tccccgcgca gcacgagcag cagcagccgc caaccgtgcg gaaacaacgc cgctccccgc
     3841 cgccagtaac gccagaaacg ccagtcatcg aagaacaggt gcgtcatcag tggcgcggtg
     3901 aggaagccgc cgaccccgct gaacagcgcg aacccgacat aggcatggaa cgccacccgc
     3961 gcgcaggccc agccgaggcg ctggcggcgc gcaggtgccc gcgccggcac cgggggcggc
     4021 gtccagtaca cgccgcgctc gagcgcgatg gtcaggtgct cggaggactg cgcgacgatc
     4081 gtttcgggca tggtgtcgga cggaggctgc acggggacgc ggaccaggga tttggcgcgc
     4141 agtatagtcg cgcggcgcgt ccgggcgcac cgggatgcgt caacgcgcca tgcccgcctg
     4201 cgctggccag tgggctacgg ttcatgaacg gatcgcaata cggcggagct attttccgta
     4261 atgcatacgg gatatagtcg gcagatacca cacccggagt ccagcatgcc cgccacgctc
     4321 gccagcgccg aaccgcgtcg cgcccgtggc cgaccacgcc ggctcacacc cgaggccgtg
     4381 ctcgatgccg cgctggcgct gttcgaggac gagccggcgg cggaattcac gctcggtcgg
     4441 ctggcacggc gcctgggcgt gccgatcacc tctctgtaca cctgtttccc caaccgcgac
     4501 gccctgctcg atgccgcggc cgatcgcgtg ttcgctcgct tcgactggaa acccgagcca
     4561 cggtcaccgt ggaccgccgt gctgctgtcg tggatgcacg cggtgcagcg gcatttcgaa
     4621 cgctacccga tcgcgcagca gctgctgacc tgggataggc gcatcagccc ggcgtggctg
     4681 cgcgtgctgg cgccgatcat cacgctgcta catgcccagg ggctgcgcgg ccgcgcgctg
     4741 gtgcagacct tcagctggct caacagcggc acgatcggcc tgatcatgtc gcacgcctac
     4801 aacgcacgcc atcaggccag gccggcgctg ggcgccgccg agctcgccgc cgacggcgaa
     4861 ggcctcggcg cacaactcga attctggagc cacgtgaacg aactggacca cgagcagtcg
     4921 ctggacgcgg gcttccgcgt gctggtggcc ggcgtggccg cggcattgcc ggctcagaaa
     4981 cgctgaaaca aaccaaaaca ccccgggagg acgaagatga gcgatatgtt gatcaagggc
     5041 ggcacggtga tagacggtac gggtgcgccg gcgcggcgcg cggacgtacg cgtgcgcaac
     5101 ggcgtgatcc ggcgcatcgc acccgatctc gcgccggaat ccggcgagcg cgtgttcgat
     5161 gcgagcggct gccaggtgac gccgggcttc atcgagagcc acacccactt cgacggcacg
     5221 atgtggtggc agcccgacat ggatccgctg cccggctacg gcgtcacgac cacgatcatg
     5281 ggcaactgcg gcttctccgc ggcacccgtg cacgaggacc cgaaagtgcg cggcgagatg
     5341 gtctacatct tctcgttctt cgaggacatc cccgagaagc ccttcctgaa gcaactgccg
     5401 tgggactggc g
//
LOCUS       DAORUQ010000198         5377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18508_length_5377_cov_5.446637, whole genome shotgun sequence.
ACCESSION   DAORUQ010000198 DAORUQ010000000
VERSION     DAORUQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18508_length_5377_cov_5.446637"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLW13_13715"
     CDS             <1..1134
                     /locus_tag="PLW13_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQY71487.1"
                     /translation="VLGVALLIVVLSVMNGFEREVRERILSLVPHVSLQPIEPVADWQ
                     PLAAAVEAHPEVVAAAPYVDLQGLLMYRGKVVPALLHGADAAAERRISAIDRYLESGS
                     LDALAEPDSVLLSRALAQRLGVAPGASLLVLVPEVNAEGQVVPRAQRMRLAGIFNTGT
                     EVDNTLALIGLDAATRLRGQPGVVQGVRFQVRDLFTAPRVQREVIQGLGIGIYGLDWT
                     RTHGNLYEAIQLSRNMVGILLFLIIAVAVFNVVSTLFLIVKDKEGDIAILRTLGASPA
                     AIMGVFVVQGTLIGLAGALAGGVIGSLLSLGITDAVALLERLLGVQFLKAEVYPVSYL
                     PSDLAWRDVLEVCAVALAMSFVATLYPSWRAARMQPAEVLRYE"
     gene            complement(1125..1658)
                     /locus_tag="PLW13_13720"
     CDS             complement(1125..1658)
                     /locus_tag="PLW13_13720"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HQY71488.1"
                     /translation="MPRRTLRRFLPQPHFVREHKSLRLLALVLHDPNLFHLNRRSVSL
                     AAFVSVFVGLQPVPMHMLVAAVAAVWLRCNITLAVIGVWVSNPLTLPAIVYAEYALGA
                     RLMGIPAGSGGFEMSVAWLEHSLHDIWEPLFLGSILGGLVLGTLAWAATRVLWRLAVQ
                     LKWRARRRERARRPPHS"
     gene            complement(1671..2216)
                     /locus_tag="PLW13_13725"
     CDS             complement(1671..2216)
                     /locus_tag="PLW13_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HQY71489.1"
                     /translation="MPRRTIRRLLPKPHAIRDHKSLRLVRERLHDPNLWHLNRHSVSV
                     AAFVGIFVAFIPVPMQMLIAAAGAIWLRCNITLAVALVWLTNPLTMPPIFYATYRLGA
                     WLMDIPASAAPFELSVEWIEQSLSDIWPPLLLGSLLCGVVLGTLAWLAMRVLWRVGVQ
                     LKWRSRLRERRNRPPREPPAP"
     gene            2411..4723
                     /locus_tag="PLW13_13730"
     CDS             2411..4723
                     /locus_tag="PLW13_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQY71490.1"
                     /translation="MIAGMLAGAAGIVIVTRLPVLPGLLPVGGAVLLALVLACWCRPL
                     PRLAACLVLGLAWGCLRGHELLARVLPAQLEGRDLPVRLCVEGLPLLRHDERGETWRL
                     RARVLDPLPGASGPLARWQGRRVELGWYGPAAFAPGEAWAMTLRLRAPRGFVNPGGYD
                     YQAWLLGQGIDATGYAVNKTPAVRLDDALCRVPLDRWRYRLRARLEGLLAHEPQLGKL
                     LAVTLGDASRFTPADWALFARTGTTHLMVISGMHITLVAMLVMAFAGFAARAWPRALL
                     VLPARSWGMLAAAPATLAYGALAGMGVSVQRALLMVGVLFLFGLRERHLQPWLAYALA
                     LLAVLVAQPLAAMQTGFWLSFITVGALLLAFAGRLERPSRAQLLWLPQLVVGVALWCP
                     LLLAGQAQAPLAPLVNALAIPLADLVVVPAALAGCLLLALGDAFAWPLLKLALLGLEL
                     LTWLLQRAAAISPELPALAPAPDAWRIAFALLGTVWLLLPRGFPARAAGVALLLPLLL
                     PAREPPPLLELLMLDAGQGSAVVVRTARHSLVYDAGPRFSERFEAGGAIVAPALARSG
                     VRRLDRLVISHADNDHSGGAAGLFAALPVNDALGAEGIEGLAARSCERGMRWEWDGVA
                     FAVLHPRAGLPGSDNNRSCVLRIAAAGRVILLAGDIEAEAEAVLLASAREQLRADMLL
                     VPHHGSRSSSTAAFVAAVQPRYALVSAGHRNRFGHPHPDVLARYRAAGAEILETARHG
                     AVRLQVDAHGVIAPPERWRIDHRRFWYAND"
BASE COUNT          671 a         1873 c         1981 g          852 t
ORIGIN      
        1 gtgctcggcg tggcgctgct gatcgtggtg ctgtcggtga tgaacggctt cgagcgcgag
       61 gtgcgcgagc gcatcctgtc gctggtgccg cacgtctcgc tgcagccgat cgagccggtc
      121 gcggactggc agccgctcgc ggccgcggtc gaggcgcatc ccgaggtcgt ggccgccgcg
      181 ccctacgtcg acctgcaggg cctgctgatg tatcgcggca aggtggtgcc ggcgctgctg
      241 cacggcgccg atgccgcggc cgagcggcgc atctccgcga tcgaccgtta cctcgaatcg
      301 ggcagcctcg acgcgctggc cgagcccgac agcgtgctgc tcagccgcgc gctcgcgcag
      361 cgcctcggtg tcgcccccgg cgccagcctg ctggtgctgg ttcccgaggt gaacgccgag
      421 ggccaggtgg tgccgcgcgc gcagcgcatg cggctcgcgg gcatcttcaa caccggcacc
      481 gaggtcgaca acacgctggc actgatcggc ctggatgccg ccacgcggct gcgcggccag
      541 cccggcgtgg tgcagggcgt gcgcttccag gtgcgtgacc tgttcaccgc gccgcgcgtg
      601 cagcgcgagg tgatccaggg gctgggcatc gggatctacg gcctcgactg gacgcgcacc
      661 cacggcaatc tctacgaggc gatccagctc tcgcgcaaca tggtcggcat cctgctgttc
      721 ctgatcatcg cggtcgccgt gttcaacgtg gtgtccacgc tgttcctgat cgtgaaggac
      781 aaggagggcg acatcgcgat cctgcgcacg ctgggcgcga gcccggcggc gatcatgggc
      841 gtgttcgtgg tccagggcac gctgatcggg ctggcgggtg cccttgccgg cggcgtgatc
      901 ggcagcctgc tgtcgctggg catcaccgac gcggtcgcgc tgctggaacg cctgctgggt
      961 gtgcagttcc tgaaggccga ggtctacccg gtgagctacc tgccctcgga tctcgcgtgg
     1021 cgcgacgtgc tcgaggtctg cgccgtggcg ctcgcgatga gcttcgtggc cacgctctat
     1081 ccctcctggc gcgcggcgcg catgcagccc gccgaggtgc tgcgctacga atgaggcggg
     1141 cgccgtgcgc gttcgcgccg ccgcgcgcgc cacttcaact gcaccgccag gcgccacagc
     1201 acccgcgtgg cagcccaggc aagcgtgccc agcaccagtc cgcccaggat gctgccgagg
     1261 aacagcggtt cccagatgtc gtgcaggctg tgctcgagcc acgcgacgct catctcgaag
     1321 ccgccgctgc cggccgggat gcccatcagg cgggcgccga gcgcgtattc ggcgtagacg
     1381 atggcgggca gcgtcagcgg gttcgacacc cacacgccga tcaccgccag cgtgatgttg
     1441 cagcgtagcc acaccgccgc cacggccgcg accagcatgt gcatcggcac cggctgcagc
     1501 cccacgaaga cgctcacgaa cgccgccagc gagaccgagc ggcggttcag gtgaaacagg
     1561 ttcggatcgt gcagcaccag cgcgagcagg cgcagcgact tgtgctcgcg gacgaagtgc
     1621 ggctgcggca ggaatcgccg gagcgtgcgg cgcggcatcg tggctttgcg ctatggcgcg
     1681 ggcggttcgc gcggtggccg gttgcggcgc tcgcgcaggc gtgagcgcca cttcagttgc
     1741 acaccgacgc gccacagcac gcgcatggcc agccaggcca gcgtgcccag caccacgccg
     1801 cacagcaggc tgccgagcag cagcgggggc cagatgtcgc tcaggctctg ctcgatccat
     1861 tccacgctca gctcgaacgg ggcggcgctg gcggggatgt ccatcagcca ggcgccgagg
     1921 cgataggtcg cgtagaagat cggcggcatc gtcagcgggt tcgtcagcca caccagcgcc
     1981 acggccagcg tgatgttgca gcgcagccag atcgcgcccg cggcagcgat cagcatctgc
     2041 atcggcaccg gtatgaacgc cacgaatatg ccgacgaatg ccgcgaccga gaccgagtgc
     2101 cggttcaggt gccacaggtt cgggtcgtgc aggcgctcgc gcaccaggcg cagcgacttg
     2161 tgatcgcgga tggcgtgcgg cttgggcagc aggcgccgga tggttctgcg cggcatcgat
     2221 ggcttccggg cgggctctgc gggcgttgac ggggcggcgg tgcgcggggc gagccggcgc
     2281 cgaagcatag gccgcagcgc ctgcccttac aagattgcaa ttgttgcggc gctgcgcctc
     2341 aatgcgctca cggccggcgc cgcgccatgc gtgccccggg cgtctcgtcc aaaggattgg
     2401 agaggctcgc atgatcgcag ggatgctcgc cggcgcggcc ggcatcgtca tcgtcacccg
     2461 ccttcccgtc ctgcccggcc tgctgccggt cggcggcgcg gtgctgctcg cgctcgtgct
     2521 ggcatgctgg tgccggccac tgccgcggct ggccgcctgc ctcgtgctgg gcctcgcctg
     2581 gggttgcctg cgcggccatg aactgctggc gcgcgtgctg ccggcgcaac tcgaggggcg
     2641 cgatctgccg gtacggctct gtgtcgaggg cctgccgctc ctgcgtcacg acgagcgcgg
     2701 ggaaacctgg cggctgcgcg cgcgcgtgct cgatccgctg cccggggcct cgggcccgct
     2761 cgcccgctgg cagggacggc gggtcgagct cggctggtac ggtccggcgg cattcgcgcc
     2821 cggcgaggcc tgggccatga cgctgcgcct gcgcgcgccg cgcggcttcg tcaatcccgg
     2881 cggttacgat taccaggcct ggctgctggg gcagggtatc gatgccacgg gctacgcggt
     2941 gaacaagacg cccgcggtgc gtctggacga tgccctgtgc cgggtgccgc tggaccgctg
     3001 gcgctaccgc ttgcgcgccc gcctcgaggg cctgctcgcg cacgagccgc agctgggcaa
     3061 gctgctcgcg gtcaccctgg gggatgcctc gcgtttcacc cccgccgact gggcgctgtt
     3121 cgcgcgcacc ggcaccacgc acctgatggt gatcagcggc atgcacatca ccctggtggc
     3181 catgctggtg atggcgttcg ccgggttcgc ggcgcgggca tggccgcggg cgctgctggt
     3241 gctgccggcg cgcagctggg gcatgctcgc ggcggcgccg gccacgctcg cctacggcgc
     3301 gctcgccggc atgggcgtgt cggtgcagcg cgcgctgctg atggtcggcg tgctgttcct
     3361 gttcggcctg cgcgagcggc acctgcagcc ctggctcgcc tatgcgctgg cgctgctcgc
     3421 cgtgctggtg gcgcagccgc tggccgcgat gcagaccgga ttctggctga gcttcatcac
     3481 cgtgggtgcg ctgctgctgg cctttgccgg gcggctggag cggccttcgc gcgcgcaact
     3541 gctgtggctg ccgcagctgg tggtgggcgt ggcgctgtgg tgtccgctgc tgctcgccgg
     3601 gcaggcgcag gcgccgctgg caccgctggt caatgccctc gcgattccgc tcgcggacct
     3661 ggtggtcgtg ccggcggcac tggccggctg cctgctgctg gccctgggcg atgccttcgc
     3721 ctggccgctg ctgaagctcg cgctactcgg cctggagctg ctgacgtggc tgctgcaacg
     3781 cgccgccgcg atcagcccgg agctgcccgc gctggcgccg gcgccggatg cctggcgcat
     3841 cgcgttcgcg ctgctcggca cggtgtggct gctgctgccg cgcggattcc ccgcgcgcgc
     3901 cgcgggcgtg gcactgctgc tgccactgct gctgcccgcg cgcgagccgc cgccgctgct
     3961 ggaactgctg atgctggacg cgggccaggg cagcgccgtg gtcgtgcgca ccgcccgtca
     4021 cagcctggtc tacgacgccg ggccgcgctt cagcgagcgc ttcgaggccg gcggggcgat
     4081 cgtggcgccg gcgctggcgc gcagcggtgt gcgccggctc gatcgcctgg tgatcagcca
     4141 tgccgacaac gaccactcgg gcggggcggc ggggctgttc gcggcgctgc cggtaaacga
     4201 cgcgctcggc gccgagggca tcgagggcct cgcagcgcga tcctgcgagc gcggcatgcg
     4261 ctgggagtgg gacggcgtgg cattcgcggt gctgcatccg cgcgcgggat tgccgggctc
     4321 ggacaacaac cgttcctgcg tgctgcgcat cgcggcggcc ggacgcgtga tcctgctcgc
     4381 cggcgatatc gaggccgagg ccgaggcggt gctgctcgca tcggcgcgcg aacaattgcg
     4441 cgccgatatg ttgctggtgc cccatcacgg cagccgcagt tcctccaccg ccgccttcgt
     4501 cgcggccgtg cagccgcgct acgcgctggt atcggccggc catcgcaacc gcttcggtca
     4561 tccgcatccg gacgtgctcg cgcgctaccg tgcggcgggc gcggagatcc tcgagaccgc
     4621 gcgccacggc gcggtgcgct tgcaggtcga tgcgcacggc gtgatcgccc cgcccgagcg
     4681 ctggcgcatc gaccatcgac ggttctggta tgcgaatgac tgatcgatgc ttgtcgccgc
     4741 gcacaattca tggatggctg gtttggagtg tcactggctg cgctggccga acttgccgtg
     4801 cgggggtcgc agcaagtacg tccctgtatg ctcgagcgcc gcttccatgc ggcgcacgcc
     4861 ccctcacagc aagttcggcc agcgcagccg ctcggcagac gcagtgccgc cgcatgaatc
     4921 ttcagacggt cctgcgtgtt tcgaagatcc accagggcaa ggcgtacgag cactcaccgg
     4981 tgcagcggac cttctgtcgc catggatggc gacaaatcgg caggactgcc gatttggacg
     5041 ccgtagcgaa gcgcaggcgc ccgaagggcg agcgccatgg agggcgctcg tcccagagag
     5101 cccacaggga ggtgttcacg gcgtgtccgc ggcgccgatg agtgctcgtg cgccgtcggt
     5161 tggctccagg tccaatcatt ggaccgcgac cgtcccgatg ccggcatcgt aaatcatgcg
     5221 tagatgatcc accgtggcta cggatgcacg gcgtcaacag tgcaatcgag gctgccgctg
     5281 cccagtcgcg cctgcacgcg cgcgccgggg ctggtgtcgg ttgccgcgcg cagcacgcgg
     5341 ccctcggcgt cgctgacgat cgcgtagccg cgcgcca
//
LOCUS       DAORUQ010000199         5357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18621_length_5357_cov_5.612410, whole genome shotgun sequence.
ACCESSION   DAORUQ010000199 DAORUQ010000000
VERSION     DAORUQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_5357_cov_5.612410"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLW13_13735"
     CDS             <1..278
                     /locus_tag="PLW13_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71491.1"
                     /translation="GEAGKVKRLRNSSQARIAPCDMRGRLLGEWAEAHAVVVKDAAET
                     DAAYRALRDKYGWQMGVLDFFSRLGGKIGQRAVIRIRAGRAPGPKRR"
     gene            complement(295..954)
                     /locus_tag="PLW13_13740"
     CDS             complement(295..954)
                     /locus_tag="PLW13_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HQY71492.1"
                     /translation="MGLAVIKESVASLDLARINRELAGAPAEDVVRWALGLGLRPFMS
                     TSFGPNAALMLDLVSGIDPGVPVVWVDTGYNLRDTYVVADKLMKLLPLNMKIYTPRMS
                     AERWNALYGGIPQLDEAELHEQFTRNIKLEPFERALAELAPELWFTGIRRGETEFRKT
                     LDIVSWDERRGILRVAPIFDWSEEDVARYVESRKLPSCKHYFDPTKVLDNRECGLHTG
                     L"
     gene            1155..2360
                     /locus_tag="PLW13_13745"
     CDS             1155..2360
                     /locus_tag="PLW13_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71493.1"
                     /translation="MNTCIRSLAREHVPYAGVFAHGMGQSMVFAMLPALGREVGFAEV
                     AIGTIVSMSSLVFFLGSRAWGRISDRWGRKPVILCGLWGYSIGTLAFALLFGLGIYGV
                     LGGTLLYGLIMGTRMAQAVVMSGTAPGTAAYIADTTTPVTRASGMGRLAAAANIGSIM
                     GPAFAGLLAAFSLLLPLVFAALVAGAAALLVNRLLPHTHVLPPSHQSGAKLRLFDPRF
                     AACLALGMAVFTGFSIVQQTLAFRIQDTLLLDGRHTAQTYGFTMMISAAAALLAQTIL
                     VQRVKLAPMLLLRIGMPLLACAFALLIWAASLPAFGMAMVLMGLGLGLCGPGFNAAVS
                     LAVSAREQGAAAGIASAIPSLGFILGPVAGTWLYQVNPHYPYLLITLILLPACVGAFR
                     IRQHVHIEP"
     assembly_gap    2367..2466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2467..3200)
                     /locus_tag="PLW13_13750"
     CDS             complement(<2467..3200)
                     /locus_tag="PLW13_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY71494.1"
                     /translation="MAKRSPARGKPYQRPNPEERTYRDRKTRNGRRPDSDGAVIVDLN
                     ARAHNLHTAYAAPRVPAPLEARTPSQKRYINAIRNHCLTFGIGPAGTGKSYCAGALAA
                     QALEAGQIDRIILTRPAVEAGEQLGFLPGDLNEKFSPYIDAFRDILNARLGAGTVDYC
                     LRHGRIVASPLAYMRGKTFDERTFVVLDEAQNTTPAQMKMFLTRIGEESRVVVNGDIR
                     QSDIRGPNGLADAIERLRGVPSVYVHR"
     gene            3596..5260
                     /locus_tag="PLW13_13755"
     CDS             3596..5260
                     /locus_tag="PLW13_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71495.1"
                     /translation="MFRPAPWLALHRSLGLDPPPAFDDRPLAGHLALHAAHRPEAVAI
                     QFHDRGIGYGELFTQSLRLATALRALGVAKGDVVGLHLPNVPQYLVAVAALSRLGAVG
                     TGVSPLLAPGEIAFQLKDAGVKVLLTLDALAAAVLPRITDMPAGLHTVIVTGAGESLG
                     LPCGPAPELPGLRTMRYPDLLGAAKADCPQTPVTGDDTFMIQYTGGTTGRPKGAMLSI
                     RNLMYNPQQTEAYAGWSGGYDEVLMSAFPLFHIAGLSVHVAALRYGCRTLLIPDPRDM
                     DFICRQLQRFAPTRIAAVPTLYQMLVNTPAFREVDFSRLKIALSGAAPLPLEDRKRIE
                     SIIGANRLSDVFGMTETGPVHVCNPPMRCKPTAVGIPVAATETRIVDLETGTREMPFN
                     EPGEIIASGPQVMKGYLNLPGESARALRVLDGRTWMFTGDVGYMDEEGYVHVCDRAKD
                     MLIVGGYKVFSVEIEDKLKALDCIAQSAVIGTPDTARPGNDIVNLYVELTPEARARDE
                     EAVRAEILAFCRENMAPYKVPKHLILIEQIPVTAVGKIDKKALRAR"
BASE COUNT          786 a         1791 c         1771 g          909 t
ORIGIN      
        1 ccggcgaggc cggcaaggtg aaacggctgc gcaattcctc gcaagcgcgc atcgcgccct
       61 gcgacatgcg cggcaggctg ctcggggagt gggcagaggc ccacgcggtg gtggtcaagg
      121 atgccgccga gaccgatgcc gcctaccgcg cgctgcgcga caagtacggc tggcagatgg
      181 gcgtgctgga tttcttttcg cgcctgggcg gcaagatcgg gcagcgcgcc gtgatccgca
      241 tccgcgcggg gcgcgcgccg gggccaaaac ggcgctgagg cgcaagcgcg gggctcagag
      301 ccccgtgtgc aggccgcact cgcggttgtc gagcaccttg gtagggtcga agtagtgctt
      361 gcacgagggc agtttgcggc tctcgacgta gcgcgccacg tcctcctcgc tccagtcgaa
      421 gatcggcgcg acgcgcagga tgccgcgccg ctcgtcccac gacacgatat cgagggtctt
      481 gcggaattcc gtctcgccgc ggcggatgcc ggtgaaccac agttccggcg ccagctcggc
      541 cagcgcgcgc tcgaagggct cgagcttgat gttgcgcgtg aactgctcgt gcagctcggc
      601 ctcgtcgagt tgcgggatgc cgccgtagag cgcgttccag cgctcggcgc tcatgcgcgg
      661 cgtgtagatc ttcatgttca gcggcagcag cttcatcagc ttgtcggcca ccacgtaggt
      721 gtcgcgcagg ttgtacccgg tatcgaccca taccaccggg acaccggggt cgatgccgct
      781 gaccaggtcg agcatcaatg ccgcgttggg accgaagctc gtgctcatga acggacgcaa
      841 ccccaggccg agcgcccagc gcaccacgtc ctcggccggc gcaccggcga gttcgcggtt
      901 gatgcgggcg agatccagag aggcgacgga ttccttgatg acggccaacc ccatggctat
      961 ggtccgggta gctgaagtgc ccggccatta taagaaaatc atctctgtaa cctaatatcg
     1021 atgctgcata tggttatttc ggcacacgcg cagcgcacgc atttgtgccg cttttgctgc
     1081 cgctggccgg caagcccgct accattcccg gcccgacccc gcgaagcgct gccgtgccct
     1141 cccgagaccg cgccatgaac acctgcatac gctcgctcgc gcgcgagcac gtgccctatg
     1201 ccggcgtgtt cgcgcacggc atgggccaga gcatggtgtt cgcgatgctc cctgcgctgg
     1261 gacgcgaggt cggcttcgcg gaggtcgcga tcggcaccat cgtctcgatg tcctcgctgg
     1321 tgttcttcct cggcagccgc gcatggggac ggatcagcga ccgctggggc cgcaagcccg
     1381 tgatcctgtg cggactctgg ggctacagca tcggcacgct ggccttcgcg ctgctgttcg
     1441 ggctggggat ctacggcgtg ctgggcggca cgctgctcta tggcctgatc atgggcacgc
     1501 gcatggcgca ggcggtggtg atgtccggga cagcgcccgg cactgcggcc tacatcgccg
     1561 acaccaccac gcccgtgacg cgcgcctcgg gcatgggacg actggcagcg gccgccaaca
     1621 tcggctcgat catgggtccg gcgttcgcgg gcctgctggc cgcgttcagc ctgctgctgc
     1681 cgctggtgtt cgccgcgctg gtggcgggcg ccgcggcgct gctggtcaat cgcctgctgc
     1741 cccacacgca cgtgctcccg ccatcgcacc agtcgggggc gaaactgcgg ttgttcgacc
     1801 cgcgctttgc cgcctgcctg gcgctcggca tggcggtgtt caccggcttt tcgatcgtgc
     1861 agcagacgct ggccttccgc atccaggaca ccttgctgct ggacgggcgc cacacggccc
     1921 agacttacgg cttcacgatg atgatcagcg ccgccgcggc gctgctcgcg cagacgatcc
     1981 tcgtgcagcg tgtcaagctc gcaccgatgc tgctgctgcg catcggcatg ccgctgctcg
     2041 cctgcgcctt cgcgctgctg atatgggccg cctcgctccc ggcgttcggc atggcgatgg
     2101 tcctgatggg actcgggctc ggcctgtgcg ggcccggctt caatgcggcc gtgtcgctgg
     2161 cggtgagtgc gcgcgaacag ggagcggctg ccggcatcgc cagcgcgata ccctcgctgg
     2221 gtttcatcct cggaccggtc gccggcacct ggctctacca ggtgaacccg cactacccct
     2281 atctcctcat caccctgatc ctgctgccgg cctgcgtggg cgcgttccgc atccgccagc
     2341 acgtgcacat cgaaccgtag gtccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnncggt gcacgtagac gctgggcacg ccgcgcagcc gctcgatcgc atcggccagc
     2521 ccgttggggc cgcggatgtc gctctggcgg atgtcgccgt tgaccaccac cctgctctcc
     2581 tcgccgatgc gcgtgaggaa catcttcatc tgcgccggcg tggtgttctg cgcctcgtcg
     2641 agcaccacga aggtgcgctc gtcgaaggtc ttgccgcgca tgtacgcgag cggcgaggcc
     2701 acgatgcgac cgtggcgcag gcaatagtcg accgtgccgg cgcccaggcg cgcgttgagg
     2761 atgtcgcgaa aggcatcgat gtagggcgag aacttctcgt tgagatcacc cggcaggaag
     2821 ccgagctgct caccggcctc gaccgcgggg cgcgtgagga tgatgcggtc gatctgcccg
     2881 gcttccagcg cctgcgccgc cagcgcgccc gcgcaatagc tcttcccggt gccggcgggg
     2941 ccgataccga aggtcaggca gtgattgcgg atggcgttga tgtagcgttt ctgggagggt
     3001 gtccgggctt cgagtggcgc cgggactcgg ggggcagcgt aggcggtgtg caggttgtgg
     3061 gctcttgcat tgaggtcgac gatgactgcg ccgtcgctgt ccggtcgtcg tccgttgcgc
     3121 gttttcctgt ctctgtaggt cctctcctcc gggttcggtc gctggtaggg cttgcctcgt
     3181 gcgggggatc ttttcgccat aaatacgaat acctcttcga tatgggtatg gagaccggat
     3241 ctcggcgctg cgctgcgatt ttcctgggcg gcgggttgct cctgtgcggc tggtgttgca
     3301 acaaataatc atcagcaaat atgaaaattg caagacatgc gcgcaaatta aatggcggct
     3361 tttgcacacg aacccatcgc gcccgagctg cgcgggcgca gcgccgcgac gaacgcacca
     3421 tccttgcgca caagctcgcg tgtttgcacc ggactggatc gtttttcccg cggttttcgc
     3481 gggcacgccg tatcggagcg tgccggcagg cgctggccat tttcggcgag gttgtcaggc
     3541 tccggaagca acgatagtct tgcgcgacaa acgataatca caggacacgc gccacatgtt
     3601 ccgccccgct ccctggctcg ccctgcaccg ctcgctcgga ctggatccgc cacccgcctt
     3661 cgatgaccgc ccgctggccg ggcacctcgc gctccatgcc gcgcacaggc ccgaggcggt
     3721 ggcgatccag ttccacgacc gcggcatcgg ctacggcgag ctgttcacgc aaagcctcag
     3781 gctcgccacc gctttgcgcg cgctcggcgt cgcgaagggc gacgtggtcg gcctgcacct
     3841 gccgaacgtg ccgcaatacc tggtggcggt ggccgcgctg tcgcgcctcg gcgccgtcgg
     3901 caccggagtc tcgccactgc tcgcccccgg cgagatcgcc ttccagctga aggacgccgg
     3961 cgtgaaggtc ctgctcacgc tggacgcgct ggccgctgcc gttttgccgc gcatcaccga
     4021 catgccggcg ggcctgcaca cggtgatcgt gaccggcgcc ggcgagagcc tcgggctgcc
     4081 ctgcggaccc gcgccggagc tgcccggcct gcgcacgatg cgttatcccg acctgctcgg
     4141 cgcggcgaag gccgactgcc cgcagacccc ggtcacgggc gacgacacct tcatgatcca
     4201 gtacaccggc ggcaccaccg ggcggcccaa gggcgcgatg ctctcgatcc gcaacctgat
     4261 gtacaacccg cagcagaccg aggcctacgc cggctggagc ggcgggtacg acgaggtgct
     4321 gatgtcggcc ttcccgctgt tccacatcgc ggggctctcg gtgcacgtgg cggcgctgcg
     4381 ctacggttgc cgcacgctgc tgatcccgga tccgcgcgac atggacttca tctgccgcca
     4441 gctgcagcgc ttcgcgccca cgcgcatcgc cgcggtgccc acgctctacc agatgctggt
     4501 caacacgccc gcattccgcg aggtggattt ctcgcgcctg aagattgcac tctcgggcgc
     4561 agcccccttg ccgctcgagg accgcaagcg catcgagtcg atcatcggcg cgaaccggct
     4621 ctccgacgtg ttcggcatga ccgagaccgg acccgtgcac gtgtgcaacc cgccgatgcg
     4681 ctgcaagccg accgcggtgg gcatacccgt cgcggccacc gaaacgcgca tcgtcgatct
     4741 cgagaccggc acgcgcgaga tgccgttcaa cgagcccggg gagatcatcg ccagcggccc
     4801 gcaggtgatg aagggctacc tgaacctgcc cggggaatcc gcgcgcgcgc tgcgcgtgct
     4861 cgacggccgc acctggatgt tcaccggtga cgtcggctac atggacgagg aaggctacgt
     4921 ccacgtctgc gaccgcgcca aggacatgct gatcgtgggc ggctacaagg tgttctcggt
     4981 ggagatcgag gacaagctga aggcgctgga ctgcatcgcg cagtcggcgg tgatcggcac
     5041 ccccgacacg gcgcgacccg gcaacgatat cgtgaacctc tacgttgagc tcacgcccga
     5101 ggcgcgcgcc cgcgacgagg aagcagtgcg cgccgaaatc ctcgccttct gccgcgagaa
     5161 catggcgccc tacaaggtgc cgaagcacct gatcctgatc gagcagatcc ccgtcaccgc
     5221 ggtgggcaag atcgacaaga aggcgctgcg ggcgcggtag gcccggcttc agagggtgtc
     5281 gcaaaggcca tcaggttgcg aacgagcggc ctggtgcgtc gctggctgtg ctgcccgcca
     5341 ttgtcctgcg gggcacg
//
LOCUS       DAORUQ010000200         5342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_18721_length_5342_cov_5.222432, whole genome shotgun sequence.
ACCESSION   DAORUQ010000200 DAORUQ010000000
VERSION     DAORUQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18721_length_5342_cov_5.222432"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLW13_13760"
     CDS             <1..271
                     /locus_tag="PLW13_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71496.1"
                     /translation="ALPADASGFLAWQQPLAAALAGAGFAIERRPFRAHLSLGRWRSR
                     SGCPPTADFALDLEGSATQVCLYESREGRYRPLFSAPCRGSGPGA"
     gene            264..1871
                     /gene="murJ"
                     /locus_tag="PLW13_13765"
     CDS             264..1871
                     /gene="murJ"
                     /locus_tag="PLW13_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQY71497.1"
                     /translation="MPESRARVRTAGRLLRSSLVVGGMTMISRVAGLVRDVVIASLLG
                     ANANADAFFVAFRVPQFLRRLFAEGAFAQAFVPVLAEYRATRPAEEVKRLVDAVSGVL
                     GAALLAVTALAVLAAPVVTLVFAPGFAQMPQKLALTAELIRITFPYLFFISLTGFAGA
                     VLNSYARFAVPAITPLFLNLTLIAGALVASRWFAEPVFALAWAVFMAGLIQLLFQVPF
                     LARIRMLPRPRLDTRHPGVGRILRLMLPAMFGVSVSQINLLLDTVLASFLPSGSVSWL
                     YYSERLTELPLGVFGIAIATVILPGLSREYSTQSNAEYRRTLDWAIRMILAIGLPASV
                     ALVILAGPILGTLFQYGAMSARDVDMASLSLAAMALGLPAFMLIKVLATAYYSRQDTR
                     TPVLIGIKAMIANMGLNLLFVVPLHLFWQVGHMGLSLATTGAAWLNAGLLLHGLMRRA
                     VYRPEPGLLRDVGRMGVAVALMCAVLLPALLWMHELDTLHWSARVLRLGLVCLAGIAV
                     YLGALALLFPRGSAFMPTKPSDRHKSG"
     gene            1990..2931
                     /gene="ribF"
                     /locus_tag="PLW13_13770"
     CDS             1990..2931
                     /gene="ribF"
                     /locus_tag="PLW13_13770"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQY71498.1"
                     /translation="MELIRGLHNLRERHHGSVVTIGAFDGVHRGHQAVISDLLGRSRE
                     FGLPSTVILFEPLPREYFAPVRSPPRLMSFREKFMVLRHLGVDRVLRIRFDRRFSIMS
                     AHEFIESVFVKGLGARYIVVGEDLRFGHDREGNIDLAREAGEKYGYEVKATSTVEVHG
                     ERVSSTRLRAVLGAGDFEAAERMLGRPYFMTGKVVYGRQLGTTIGVPTANLALHRHRA
                     PLAGVYVAEVRIGGRLLPAVANVGTRPTVDEGIKANLEVHILDFAQNIYGRTIDVIFR
                     RQIRDEHKFASVDELRNRIADDIAVARAWFAARPQTH"
     gene            2944..>5342
                     /gene="ileS"
                     /locus_tag="PLW13_13775"
     CDS             2944..>5342
                     /gene="ileS"
                     /locus_tag="PLW13_13775"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQY71499.1"
                     /translation="MTDYKHTLNLPQTEFAMKANLAQREPQMLARWHDEDVYGQIRAA
                     RAGRPKFILHDGPPYANGEIHIGHAVNKILKDIIVKARTLDGFDAPYVPGWDCHGLPI
                     ELNVEKKVGKAGVKISHHEFRTHCRDYARSQVERQREDFIRLGVFGDWANPYLTMDFA
                     FEADIVRALGRIVANGHLEKGSKPVHWCMDCGSALAEAEVEYHEKTSPAVDVRFRAVD
                     PVALARAFGVELEPSARVSVPIWTTTPWTLPANLAVALHPQLPYVLVRAGEGEEAEYL
                     VLAEALARQALARCGIEAYEILGECAGAALEKQALRHPFLDRTVPVILGQHVTTEAGT
                     GAVHTAPGHGLDDYVVGQAYGLGVLNPVDGQGVFVEGTPHVAGLYVFKANAVIVELLR
                     EKGALLHDVAYQHSYPHCWRHKSPVIFRATPQWFVSMNRNGLLDTARGAIEDVQFVPA
                     WGKARIEGMVADRPDWCISRQRTWGVPIALFVHKQTGAPHPRTAELIEQAAQRIEQAG
                     IDAWFAIDPAELLGEDAAHYDKVTDTLDVWFDSGVTHACVLTRRPELQFPADLYLEGS
                     DQHRGWFQSSLLTASGIQGCAPYKGVLTHGFTVDSAGHKMSKSRGNVIAPQQVMKTLG
                     ADILRLWVAATDYRAEMAVSDEIFKRTADSYRRIRNTARFLLSNLAGFDPSAHALAGK
                     DMLALDRWVVDRAAQLQREIVEAYGSYQFHLIYQKLHNFCVTDLGGFYLDVIKDRQYT
                     TRADSVARRSAQTALYHVLEAMVRWIAPILSFTADEIWRCMPGERSAPVFAAQWYEAL
                     E"
BASE COUNT          766 a         1821 c         1892 g          863 t
ORIGIN      
        1 ggcgctgccc gcggacgcct cgggcttcct ggcctggcag caaccgctcg cggcggcgct
       61 ggccggcgcg ggtttcgcca tcgagcgccg tcccttccgg gcgcacctga gcctcgggcg
      121 ctggcgctcg cgctcgggtt gcccgcccac cgcggacttc gcgctcgatc tcgagggcag
      181 cgcgacgcag gtgtgcctgt acgaaagccg cgaaggccgc taccggccgc tgttcagtgc
      241 accctgccgg ggatcgggcc ccggtgcctg agtcccgggc gcgcgtgcgc accgcaggcc
      301 ggctgttgcg ttccagcctc gtggtcggcg gcatgaccat gatctcgcgc gtcgccggcc
      361 tggtgcgcga cgtggtgatc gcctcgctgc tcggcgccaa cgccaacgcg gatgccttct
      421 tcgtcgcgtt ccgcgtgccg cagttcctgc gccggctgtt cgccgaggga gcgttcgcgc
      481 aggccttcgt gccggtgctc gcggagtacc gcgccacgcg cccggcggaa gaggtgaaaa
      541 ggctggtcga tgcggtgtcc ggcgtgctgg gcgcggcgct tctggccgtc acggcgctgg
      601 cggtgctcgc cgcgccggtg gtgacgctgg tgttcgcgcc cggcttcgcg cagatgccgc
      661 agaagctggc gctcaccgcg gagctgatcc gcatcacctt cccctatctc ttcttcatct
      721 cgctgaccgg cttcgccggc gcggtgctga acagctacgc gcgcttcgcg gtgccggcga
      781 tcacgccgct gttcctgaac ctgacgctga tcgccggcgc gctggtggct tcgcgctggt
      841 tcgccgagcc ggtgttcgcg ctggcctggg cggtgttcat ggccgggctg atccagttgc
      901 tgttccaggt tccgttcctc gcgcgcatcc ggatgctgcc gcgcccgcgc ctcgacaccc
      961 gccacccggg cgtggggcgc atcctgaggc tgatgctgcc ggcgatgttc ggcgtctcgg
     1021 tgagccagat caacctgctg ctcgacaccg tgctcgcctc gttcctgccg agcggcagcg
     1081 tgtcgtggct gtattactcg gagcggctga ccgagttgcc gttgggagtg ttcggcatcg
     1141 cgatcgcgac cgtgatcctg ccggggctgt cgcgcgagta ctcgacgcaa tcgaatgccg
     1201 agtaccgccg caccctcgac tgggcgatca ggatgatcct ggcgatcggc ctgccggcca
     1261 gcgtcgcgct ggtgatcctg gccggcccca tcctgggcac gctgttccag tacggggcca
     1321 tgagcgcccg cgacgtcgac atggcctcgc tcagcctggc ggcgatggcg ttgggtctgc
     1381 cggccttcat gctgatcaag gtgctggcca ccgcgtatta ctcgcgccag gacacccgta
     1441 cgccagtgct catcggcatc aaggcgatga tcgcgaacat ggggctcaac ctgctgttcg
     1501 tggtgccgct gcacctgttc tggcaggtgg ggcacatggg gctgtcgctc gcgaccaccg
     1561 gggcggcctg gctcaatgcc ggcctgctgc tgcacgggct gatgcgccgc gcggtgtacc
     1621 gcccggagcc gggcctgctg cgcgacgtgg gcaggatggg ggtggccgtg gctctcatgt
     1681 gcgcggtgct gctgccggcg ctgctctgga tgcacgagct ggacaccctg cactggagcg
     1741 cccgggtgct gcgcctgggg ctggtctgcc tggcgggcat cgcggtgtat ctcggggccc
     1801 tggcgctgct gttcccccgt gggtcggcat tcatgccgac aaagccctcg gacaggcaca
     1861 agtccggatg aatgcggacc cacgggaatg tccgggtgaa tccggaccca cggggaaatg
     1921 tccgcatgaa tgcggaccta ctctataatc gccggctttg gcgaaacccc cgggcgggag
     1981 cacagcggat tggaactgat caggggcttg cacaatctgc gcgagcgcca ccatggcagc
     2041 gtggtgacca tcggcgcctt cgacggggtg caccggggtc accaggccgt gatcagcgac
     2101 ctgctggggc gctcgcgcga gttcgggctg ccctcgacgg tgatcctgtt cgagccgctg
     2161 ccgcgggaat acttcgcccc ggtacggtcg ccgccgcgcc tgatgagctt tcgcgagaag
     2221 ttcatggtgc tgcgccatct cggggtggac cgggtgctgc gcatccgctt cgaccggcga
     2281 ttcagcatca tgtcggcgca cgagttcatc gagtcggtgt tcgtgaaggg cctcggcgcg
     2341 cgttatatcg tggtcggtga ggaccttcgt tttggccacg accgcgaggg caacatcgac
     2401 ctggcgcgcg aggccggcga gaaatacggc tacgaggtga aggcgaccag cacggtcgag
     2461 gtccacggcg agcgcgtcag cagcacgcgg ctgcgcgcgg tgctgggggc gggggatttc
     2521 gaggccgccg aacgcatgct cgggcggccg tatttcatga ccggcaaggt ggtgtacggc
     2581 cgccagctcg gcaccaccat cggcgtgccc acggcgaacc tcgcgctgca ccgccaccgc
     2641 gcgccgctgg ccggcgtgta cgtggccgag gtgcgcatcg gcgggcggct gctgccggcc
     2701 gtggctaacg tgggcacgcg gcccacggtg gacgagggca tcaaggcgaa cctcgaggtg
     2761 cacatcctcg acttcgcgca gaacatctac ggacgcacga tcgacgtgat tttccgccgc
     2821 cagatccgcg acgagcacaa gttcgcctcg gtggacgaac tgcgcaatcg catcgccgat
     2881 gacatcgcgg tggcgcgcgc ctggttcgcc gcgcgcccgc aaacccactg aacgaagcag
     2941 cccatgacag actacaagca caccctgaac ctgccgcaga ccgagttcgc catgaaggcg
     3001 aacctcgcgc agcgcgagcc gcagatgctg gcgcgctggc atgacgaaga cgtctacggg
     3061 cagatccgcg ccgcgcgtgc cgggcggccg aagttcatcc tgcacgacgg cccgccctac
     3121 gccaacggcg agatccacat cgggcacgcg gtcaacaaga tcctgaagga catcatcgtc
     3181 aaggcgcgca cgctcgacgg tttcgacgcg ccctacgtgc cgggctggga ctgccacggg
     3241 ctgccgatcg agctgaacgt cgagaagaag gtcggcaagg ccggcgtgaa gatcagccac
     3301 cacgagttcc gcacgcactg ccgcgactac gcgcgcagcc aggtggagcg ccagcgcgag
     3361 gacttcatcc gcctcggcgt gttcggcgac tgggccaacc cctacctgac gatggacttc
     3421 gccttcgagg ccgacatcgt gcgcgcgctg gggcgcatcg tcgccaacgg gcacctggag
     3481 aaaggctcca agcccgtgca ctggtgcatg gattgcggct cggcgctggc cgaggccgag
     3541 gtcgagtacc acgagaagac ctcgcccgcg gtggacgtgc gctttcgcgc cgtggacccg
     3601 gtggcgctgg cgcgcgcctt cggcgtggag ctcgagccct cggcgcgcgt ctcggtgccg
     3661 atctggacca ccacgccgtg gacgctgccg gcgaacctcg cggtggcctt gcacccgcag
     3721 ctgccctacg tgctggtgcg cgcgggcgag ggtgaggagg ccgaatacct cgtgctggcg
     3781 gaggcgctgg cgcgccaggc gctggcgcgc tgcggcatcg aggcctacga gatcctcggc
     3841 gaatgcgccg gtgccgcgct ggagaagcag gccctgcggc atcccttcct cgatcgcacc
     3901 gtgcccgtga tcctcgggca gcacgtgacc accgaggccg gtaccggcgc ggtgcacacg
     3961 gcgccggggc acggcctgga tgactacgtg gtcgggcagg cctacgggct cggcgtgctg
     4021 aacccggtgg acgggcaggg cgtgttcgtc gagggcacgc cgcatgtcgc tggcctgtac
     4081 gtgttcaagg ccaacgcggt gatcgtcgag ttgctgcgcg agaagggcgc gctgctgcac
     4141 gacgtggcct accagcacag ctacccgcat tgctggcgcc acaagtcgcc ggtgatcttc
     4201 cgcgccacgc cgcagtggtt cgtgagcatg aaccgcaacg ggctgctcga taccgcgcgc
     4261 ggcgcgatcg aggacgtgca gttcgtgccc gcctggggca aggcgcgcat cgagggcatg
     4321 gtcgcggacc gccccgactg gtgcatctcg cgccagcgca cctggggcgt gccgatcgcg
     4381 ctgttcgtgc acaagcagac cggcgcgccg cacccgcgca ccgcggagct gatcgaacag
     4441 gccgcccagc gcatcgaaca ggccggcatc gacgcctggt tcgcgatcga ccccgcggag
     4501 ctgctgggcg aggacgccgc gcactacgac aaggtcacgg acacgctgga cgtgtggttc
     4561 gactccggcg tcacgcacgc ctgcgtgctg acgcgccgcc ccgagttgca gttccccgcg
     4621 gacctgtacc tggagggctc cgaccagcac cgcggctggt tccagtcgtc gctgctgacc
     4681 gcgagcggca tccagggatg cgcgccgtac aagggcgtgc tgacgcacgg cttcacggtc
     4741 gacagcgccg ggcacaagat gtccaaatcg cgcggcaacg tgatcgcgcc gcagcaggtc
     4801 atgaagacgc tgggcgccga catcctgcgc ctgtgggtcg cggccacgga ttaccgcgcc
     4861 gaaatggcgg tgtccgacga gatcttcaag cgcaccgcgg actcgtaccg gcgcatccgc
     4921 aataccgcgc gcttcctgtt gtcgaacctg gcgggcttcg acccgtccgc gcacgcgctc
     4981 gccggcaagg acatgctggc gctggaccgc tgggtggtgg atcgcgcggc gcagttgcag
     5041 cgcgagatcg tcgaggccta cggctcgtac cagttccatc tgatctacca gaagctgcac
     5101 aacttctgcg tcacggatct cgggggcttt tacctcgacg tgatcaagga ccgccagtac
     5161 accacgcgcg ccgacagcgt ggcgcggcgc tcggcgcaga ccgcgctgta ccacgtgctg
     5221 gaggcgatgg tgcgctggat cgccccgatc ctgagcttca ccgccgacga gatctggcgc
     5281 tgcatgccgg gcgagcgcag cgcgccggtg ttcgcggcgc agtggtacga ggcgctcgag
     5341 ga
//
LOCUS       DAORUQ010000201         5276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19032_length_5276_cov_7.023176, whole genome shotgun sequence.
ACCESSION   DAORUQ010000201 DAORUQ010000000
VERSION     DAORUQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19032_length_5276_cov_7.023176"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLW13_13780"
     CDS             <1..294
                     /locus_tag="PLW13_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQY71500.1"
                     /translation="GVHGVGGILGALLTGVFADPQLGGAGFFDWVTMEVAPYDMLSQV
                     LIQAKSVGITLLWSGLVSLVLFKLIDLAIGLRVSEETERQGLDTVEHGERAYG"
     gene            567..1007
                     /locus_tag="PLW13_13785"
     CDS             567..1007
                     /locus_tag="PLW13_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY71501.1"
                     /translation="MTWLLLPCAAASLAANAEIYRWTDAEGRVHFGDRAPATGAAVQS
                     IEAPAGSATPDPELQRHRERSRKLLEVWDTERRDRSAAAAAAALETEERDRACAQLRA
                     ELERSRHAAYLVHESAAGAREILDSGAREQYERGLADALAAHCR"
     gene            1091..1429
                     /gene="glnK"
                     /locus_tag="PLW13_13790"
     CDS             1091..1429
                     /gene="glnK"
                     /locus_tag="PLW13_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQY71502.1"
                     /translation="MKLITAIIKPFKLDDVRSALSDIGVQGVTVSEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEIAVDDAQTDAVIEAIRAAANTGKIGDGKIFVAPLEQVIRI
                     RTGETGPDAI"
     gene            1586..1843
                     /locus_tag="PLW13_13795"
     CDS             1586..1843
                     /locus_tag="PLW13_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71503.1"
                     /translation="MSSEDEETETEAVDSDTVEAEAAAASPAREEPAETRSSEARRKL
                     REQMQAEIDAFLKSGGRIQQLEPTVSAQAAPLTATAGNEVA"
     gene            complement(1916..2629)
                     /locus_tag="PLW13_13800"
     CDS             complement(1916..2629)
                     /locus_tag="PLW13_13800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY71504.1"
                     /translation="MATRRYALITFDLDDTLWDVAPVLARAEQRVEQWMRVHCPRVPE
                     RFDRAALMELRRRLHRERPDLQHRISELRIEAMRLALLAAGHDEASARRLAARAFAVF
                     MEGRHDIEPFAGVDTVLAGLARHYVLGALTNGNADVFRLPLGRHFRFAFSAEALGASK
                     PAPGHFEAALRAADVTPERALHVGDHLEHDVLGARRAGMTAVWFNRAGRGHEGDEPAH
                     AEFRDFEELPALIERLEEE"
     gene            complement(2646..3572)
                     /gene="xerC"
                     /locus_tag="PLW13_13805"
     CDS             complement(2646..3572)
                     /gene="xerC"
                     /locus_tag="PLW13_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQY71505.1"
                     /translation="MGAGTRLARDIGSWLDYLEATRRASPHTLAAYRRDLALLASVLA
                     ERGIDDTALIDAHAIRHAVSALHRRGLGGRSLQRFLSACRGFFNHFVSLGQLGANPAL
                     GVSAPKSPRKLPTTLDVDQAAQLLDLEPASWEAVRDKAMMELFYSSGLRLAELCGLDG
                     PDLDLAGALVTVTGKGAKTRTVPVGKLAIAALKRWLEVRRDCARATQTPALFVTRRGT
                     RISVRAVQKRLAQHGRERQLAQRLHPHMLRHSFASHVLESSGDLRSVQEMLGHANLST
                     TQIYTHLDFQHLARVYDAAHPRAARKRNRNGD"
     gene            complement(3573..4298)
                     /locus_tag="PLW13_13810"
     CDS             complement(3573..4298)
                     /locus_tag="PLW13_13810"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HQY71506.1"
                     /translation="MKEEFGAILRMRATEVEADNEPGVEQVLAYLARHPDFFLRHEAI
                     LAELELPHRAGAAVSLVERQVALLRERNIESRQRLNRLLETARENDELFNKTRRLILA
                     LLEADSLERLAQVLVLGLRREFDVEHARLLLIEDAASHWQHGGERIAREAAEAALNGV
                     MRQDKPVSGPLRPQAREALFGAHGDSVLSSVVVTVAHAHPLAIIALGSGDARRFHGEM
                     GTMFMELIGDVLQRLLPRFTGPQ"
     gene            complement(4295..5125)
                     /gene="dapF"
                     /locus_tag="PLW13_13815"
     CDS             complement(4295..5125)
                     /gene="dapF"
                     /locus_tag="PLW13_13815"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQY71507.1"
                     /translation="MLLRFTKMHGLGNDFVVLDLITQSVRLDAPLIRFLADRHRGIGC
                     DQVLVVEPPTAPDMDFLYRIYNQDGSEAQQCGNGARCVAKFIRDRRLSAKRRLRVETL
                     GGPISMSCGKGNVVTVSMGIPRLSPQEIPFTAARQATVYTLEACGQPVEISAVSMGNP
                     HAVLVVDDVETAPVATLGPVLEQHPDFPERTNVGFMQVLDRGEVRLRVFERGAGETMA
                     CGSGACAAVVAGRIRGLLDERVRVHLPGGFLQIEWRGEGDPLMMTGPATTVFEGQIAI
                     "
     gene            complement(5127..>5276)
                     /locus_tag="PLW13_13820"
     CDS             complement(5127..>5276)
                     /locus_tag="PLW13_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQY71508.1"
                     /translation="AYSFAMSSNYNSRCRPAEVMVDGDTAFLVRARESFEDLVRGEQL
                     LPADG"
BASE COUNT          826 a         1788 c         1841 g          821 t
ORIGIN      
        1 ggcgtgcacg gcgtcggcgg catcctgggt gcgctgctga ccggcgtgtt cgccgatccg
       61 caactgggcg gcgcgggctt cttcgactgg gtgaccatgg aagtggctcc gtacgacatg
      121 ctgtcccagg tgctcatcca ggccaagagc gtgggcatca ccctgctgtg gtccggcctg
      181 gtgagcctgg tgctcttcaa actgatcgac ctggcgatcg gcctgcgcgt ctcggaagag
      241 acggagcgcc agggcctcga cacggtcgag cacggcgagc gcgcctacgg ctgatgaggg
      301 cacgctcccc ctcccgcgag ggggagcgtc acttgcaccc tgatccgcgg gccccggtgc
      361 ccgcaccgta ccggtctcga ttctcctgtt ggcgcccccg gggcgccctt tttttgggtc
      421 gccacaaatg gcgacagcag cattcgacga cagcagcatt cggcaaggca catgtcggca
      481 tcaatgccga cccacaagac catccccgcc aacgcgtgca tgtcttctgg ccttcgggca
      541 acatgggccg catgacgtta ctgcgcatga catggttact gctgccctgc gccgccgcga
      601 gccttgcggc gaacgccgaa atctaccgct ggaccgacgc cgagggccgc gtgcactttg
      661 gcgatcgcgc gccggcgact ggcgccgcgg tgcagtcgat cgaagcgccc gcggggagcg
      721 cgacacccga cccggaattg cagcgccatc gcgagcgcag ccgcaagctg ctcgaggtgt
      781 gggatacgga gcgccgcgac cgcagcgcgg cagcagcggc agcggcgctc gagaccgagg
      841 agcgcgaccg ggcctgcgcg cagctgcgcg ccgagctcga acggtcgcgc catgctgcct
      901 acctggtgca cgagtccgcc gctggcgcgc gcgagatcct cgacagcggc gcgcgcgagc
      961 agtacgagcg cggccttgcc gatgcgctcg ccgcgcattg ccgctgagtc gcgctgttgt
     1021 gctgtaatgc gcgccagtat aatgcgcgcc gttcagccgg ccggcgccga catgtcgcag
     1081 gagacacacg atgaagttga tcaccgccat catcaaaccc ttcaagctcg atgacgtgcg
     1141 ctcggcgctg tcggacatcg gcgtgcaggg tgtcacggtc agcgaggtca agggtttcgg
     1201 gcgccagaag ggtcacaccg agttgtatcg cggtgccgag tacgtggtgg atttcctgcc
     1261 caaggtcaag ctcgagatcg cggtcgacga cgcgcagacc gacgcggtga tcgaggcgat
     1321 ccgcgccgcg gccaataccg gcaagatcgg cgacggcaag attttcgtcg caccgctcga
     1381 gcaggtcatc cgcattcgca ccggcgagac gggtcccgac gccatctgag cgagtggatg
     1441 cgctcaggta ataagaacgc ggcatgaaat aagcaaaaag tttttgactc gcaagcggct
     1501 ggtctataag taagcgctgt gagtcgtggg tgtgatgcga cagcacggga tagcaccagc
     1561 gtgttgatga ccggaagggg aattcatgtc atctgaagac gaagaaaccg aaaccgaagc
     1621 cgttgattcg gacaccgtgg aagccgaggc agcggctgcg tcccccgcgc gcgaagagcc
     1681 ggcggaaacg cgctcctccg aggcaaggcg caagctgcgc gagcagatgc aggccgagat
     1741 agatgccttc ctcaagtccg gcgggcgtat ccagcagctc gagccgaccg tgagcgcgca
     1801 ggcggctccg ctgacggcta ccgcaggcaa cgaagtcgcc tgatccgcgc acggcgcgcg
     1861 gctgcatcgt gccgaatgcg cgtcgccgat ggcggcgcgc gcagcagttc gagtctcact
     1921 cttcttccag tctttcgatc agcgcgggaa gttcctcgaa atcgcggaac tccgcgtgcg
     1981 caggctcgtc cccttcatgt ccgcgacccg cgcgattgaa ccacactgcc gtcatgccgg
     2041 cccggcgcgc gcccagcacg tcgtgctcga ggtgatcgcc gacgtgcagc gcacgctcgg
     2101 gcgtcacatc ggccgcccgc agcgcggctt cgaaatgccc gggggcgggc ttgctcgcgc
     2161 cgagcgcctc ggcgctgaac gcgaagcgga aatggcgccc gagcggcagg cggaacacgt
     2221 cggcgttgcc gttggtcagc gcgccgagca catagtggcg cgcgagcccg gcgagcacgg
     2281 tgtcgacgcc ggcaaatggc tcgatgtcgt gccgtccttc catgaacacc gcgaatgccc
     2341 gggcggcgag tcgccgcgcc gatgcctcgt catggcctgc cgcgaggagc gcgaggcgca
     2401 tggcctcgat gcgcaattcg ctgatgcggt gctgcaggtc ggggcgttcg cgatgcagtc
     2461 gtcggcgcag ctccatcagc gccgcgcggt cgaagcgctc cggcacgcgc gggcaatgaa
     2521 cgcgcatcca ctgctcgacc cgctgctcgg cccgcgcgag cacgggcgcc acatcccaga
     2581 gcgtgtcatc gagatcgaag gtgatcagtg cgtagcgccg cgtcgccatg tgcaagctcc
     2641 cggcctcagt ccccgttgcg gttacgcttg cgcgccgcgc gcggatgcgc cgcgtcgtac
     2701 accctggcca ggtgctggaa gtcgaggtgc gtgtagatct gcgtggtcga gagattggcg
     2761 tggcccagca tctcctgcac cgagcgcagg tcgccgctgg attccagcac gtggctcgcg
     2821 aaggagtggc gcagcatgtg cggatgcagg cgttgcgcca gctggcgctc gcgcccgtgc
     2881 tgcgccaggc gcttctgcac ggcccgcacg ctgatgcggg tgccgcggcg cgtcacgaac
     2941 agcgccggcg tctgcgtagc gcgcgcgcag tcgcggcgca cttccagcca gcgtttcagc
     3001 gccgcgatcg caagcttccc gaccggcacc gtgcgcgtct tggcgccctt gccggtaacg
     3061 gtgaccagcg cgcccgccag gtccaggtcc gggccgtcca ggccgcacag ttccgccagg
     3121 cgcaggcccg agctgtagaa cagctccatc atggccttgt cgcgcaccgc ctcccagctc
     3181 gccggctcga ggtcgagcag ctgcgcggcc tggtccacgt cgagcgtcgt gggcagcttg
     3241 cgcggcgatt tcggcgcgga cacgccgagc gccgggttcg cgccgagttg ccccaggctc
     3301 acgaagtggt tgaagaagcc gcggcaggcg gacaggaagc gttgcaggct gcgcccgccc
     3361 aggccgcgcc ggtgcagcgc ggatacggcg tggcggatgg cgtgggcatc gatcagggcg
     3421 gtgtcgtcga tgccgcgctc ggcgagcacg ctggcgagca gcgcaaggtc gcggcggtag
     3481 gcggcgaggg tgtgcgggga ggcgcggcgc gtggcctcga ggtagtcgag ccagctcccg
     3541 atgtcacgcg cgagccgtgt gcccgcgccc attcactgcg gtccggtaaa gcgcggcaac
     3601 aggcgctgca gcacgtcgcc gatcagttcc atgaacatcg tgcccatctc gccgtggaag
     3661 cgccgcgcgt cgccgctgcc gagcgcgatg atcgctaacg ggtgcgcgtg cgccacggtg
     3721 acgaccaccg aggacagcac gctgtcgccg tgggcgccga acagcgcctc gcgggcctgc
     3781 ggacgcagcg gtccggagac cggcttgtcc tggcgcatga cgccgttgag ggctgcctcg
     3841 gcggcctcgc gcgcgatgcg ctcgccgccg tgctgccagt ggctggctgc gtcctcgatc
     3901 agcagcaggc gtgcgtgctc cacgtcgaac tcgcgacgca agcccagcac cagcacctgt
     3961 gccagtcgtt cgagcgaatc ggcctcgagc agcgcgagga tcagccggcg cgtcttgttg
     4021 aacagctcgt cgttctcgcg cgcggtctcg agcagccggt tcaggcgctg gcgcgactcg
     4081 atattgcgct cgcgcagcag cgccacctgg cgctccacca gcgacactgc ggcgccggcc
     4141 cggtggggca gttcgagctc ggcgaggatg gcttcgtggc gcaggaaaaa gtccgggtgg
     4201 cgcgccaggt acgccagcac ctgctccacg ccgggttcgt tgtccgcctc gacttccgtc
     4261 gcccgcatgc gcaggatggc gccgaattct tctttcatat tgcgatctgt ccttcgaata
     4321 ccgtggtcgc ggggccggtc atcatcagcg ggtcgccttc gccccgccat tcgatctgca
     4381 ggaatccgcc cggcaggtgc acgcgcacgc gctcgtcgag caggccgcgg atccggccgg
     4441 ccactaccgc ggcgcaggca ccgctgccgc aggccatggt ctcgccggcg ccgcgctcga
     4501 acacgcgcaa ccgcacctca ccgcggtcca gcacctgcat gaagccgacg ttggtgcgct
     4561 cgggaaagtc cgggtgctgt tccagcaccg gaccgagcgt cgcaacggga gcggtttcca
     4621 cgtcgtccac caccagcacc gcgtggggat tccccatcga cacggcgctg atctcgaccg
     4681 gctgtccgca ggcttcgaga gtatagacgg tggcctggcg ggcggccgtg aatggtattt
     4741 cttgcggtga gaggcgcggg atccccatgc tcaccgtgac cacgttgccc ttgccgcagc
     4801 tcatgctgat cggtccgccc agcgtttcca cgcgcaggcg ccgcttcgcg ctgaggcgac
     4861 ggtcgcggat gaacttcgcc acgcagcgcg cgccgttgcc gcactgctgc gcctcgctgc
     4921 cgtcctggtt gtagatgcgg tagaggaagt ccatgtcggg agcggtgggc ggctctacca
     4981 ccagcacctg gtcgcagccg atgccgcggt ggcggtcggc gaggaagcgg atcaacggcg
     5041 cgtcgaggcg caccgattgc gtgatcaggt cgagcacgac gaagtcgttg cccaggccgt
     5101 gcatcttggt gaagcgcagc agcatctcag ccatccgccg gcagcagctg ctcgccgcgc
     5161 accaggtcct cgaagctctc gcgggcacgc accaggaatg cggtgtcgcc gtcgaccatg
     5221 acctcggccg ggcggcagcg gctgttgtag ttggagctca tcgcgaagga gtacgc
//
LOCUS       DAORUQ010000202         5252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19157_length_5252_cov_8.280739, whole genome shotgun sequence.
ACCESSION   DAORUQ010000202 DAORUQ010000000
VERSION     DAORUQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19157_length_5252_cov_8.280739"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PLW13_13825"
     CDS             complement(<1..1196)
                     /locus_tag="PLW13_13825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71509.1"
                     /translation="MTRSFAEFRRGWAVLAASSLGVAFGASPIPYNSLGPFTRPITEE
                     FGWGRGEFQLAILWFTIAVVLVVPMVGALADRYGVRRMAILTLTLFGLSFAALALTPA
                     SIAVFYAMWFVTGFAGGGSTPVTWTRAINGWFVENRGLALAITLLGTGITAAFLPTLT
                     IWLIGNFGWRVAFLGISLLPLAVALPIAIAWFRDPPAPSAVSDDGVVTHRAEGGVTLG
                     TAVRNYRFWVLIVSILLVATGIGGTITNLVPLLEDRGFRALDAGKIAGAVGVSIMFGR
                     VFAGFMMDRFWAPGVVFPMLILPAIACLLLMNHDMGAGTAILAAILVGFASGAETDLV
                     AYLSARYFGLRHYGRIYGAQYAVFGFASGIAPPLFGFVHDRTGSYDAILATAAVAFGV
                     GAALLLT"
     gene            complement(1193..1750)
                     /locus_tag="PLW13_13830"
     CDS             complement(1193..1750)
                     /locus_tag="PLW13_13830"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQY71510.1"
                     /translation="MRVHAFAPILGAKPRLLILGSLPGIASLRAREYYAHPRNQFWPI
                     MQALFGVPADAPYARRVAALRAVGVCVWDVLAEATRPGSADAAIEPATARANGIHQLL
                     LRHRSIAVIAFNGGVAHRLFTRLVLPGIPPRHVAPLEFVTLPSTSPANARLTPAAKRR
                     RWRILLARTLDLDPQCARAAINFMP"
     gene            complement(1755..2525)
                     /locus_tag="PLW13_13835"
     CDS             complement(1755..2525)
                     /locus_tag="PLW13_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQY71511.1"
                     /translation="MSFWSALWIVFVSGTVATALGAAPVLALRELSPRLANHFISLAA
                     GVMLAASVYALLPAALHEAAQRVPGAWATPLTLGALGVGAWCVSWANRHFPHEHQFKG
                     HDGADRPHSMSFLLVTAIALHNFPEGLSVGVTIGAQDPTLTPRIATAIAIQNIPEGLV
                     VAMAMLSGGFGRARSFGLGVLTGLIETGGGIVGAAAVGLAGGLLPWALAFAAGAMLYV
                     ISAEVIPETHRRGMAGGATASLLAGFAAMVWFNEFITI"
     gene            2604..3092
                     /locus_tag="PLW13_13840"
     CDS             2604..3092
                     /locus_tag="PLW13_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY71512.1"
                     /translation="MIRINVNGVHHTVDVEPDTPLLWVLRDALGLTGTKYGCGIAQCG
                     TCTVHVGGQPTRACVLPVGSVGDMRITTIEGLHPEGRHALQLAWIAEQVPQCGYCQSG
                     QIMSAAALLAATPQPADADIDAAMAGNLCRCGTYPRIRNAIKRAAGLAGASKPRTVKG
                     AS"
     gene            3089..>5252
                     /locus_tag="PLW13_13845"
     CDS             3089..>5252
                     /locus_tag="PLW13_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY71513.1"
                     /translation="MSLARREFLALSGALCVGVALPRAAGAVKPLAAGKAIPLTAYVV
                     IEPGGLVRVLSPVSEMGQGTFAAHAAIVADELGVDYSMVRVETAMPADPYRHPGPGGA
                     PASQSTGGSWGVRRWYGPLRKGAAQAREMLIAVAAARSGLAAAEFAVSDGVVKHATSA
                     AARYTFGELAADAAKLPPPAEPLLRPRSARRHVGARLERPDIPDKVRGRTLYSSDFRR
                     PGMVYACARIAPVQGAKPTRIDRASALAVKGVIEVVALPNGAAVVASSQWAAMQGARA
                     LVIEFERTANDSLNSAAVSAAMREGLGAVSRAVLRSEGDFEVALASAARVVTADYEVP
                     YLANAPLEPWSCTAEIVADGSLDLWGPFQAQDRARTGAARTLGIDGVRVHTLPLGGGF
                     GRRLNDDGVPAAALVARAMGKPVKLFWTRETEFAAGFGRPAQMARITAALDLSGRVTG
                     LHFRASGPSLILHFAPGPPVVPDATFLDGTSVQNLDVVRYAFGAYRVDYALRHNHFPT
                     GPWRSVGATQNAFFLESFIDEIAHATARDPVQLRRELLAHDPRALRVIDEAARRGGWG
                     TPVKPGHALGFAYFECYGSLCAHVAEVSIKDGAPRVHRVVCVLDCGEVVTPDGARAQA
                     EGGIVQGLSAALGEALTLKGGAAIEQNFDTYPLLRLPDAPREIDVHFIQSGEPLGGVG
                     EPVLPPIAPAVANAVRVLTGKPVRRLPILVPGFRLFGYS"
BASE COUNT          886 a         1705 c         1870 g          791 t
ORIGIN      
        1 gtgagcagca acgcggcgcc gaccccgaac gcgaccgcgg cggtggccag gatcgcgtcg
       61 tagctgccgg tcctgtcgtg cacgaagccg aagagcggcg gggcgatgcc cgaggcgaag
      121 ccgaacaccg cgtactgggc gccgtagatg cgaccgtagt gcctgagacc gaagtagcgc
      181 gcgctcaggt aggccacgag atcggtttca gcgcccgacg caaaaccaac aagtatcgcc
      241 gcgaggatcg ctgtgcctgc gcccatgtcg tggttcatca gcagcaggca cgcgatcgcc
      301 ggcaggatca gcatggggaa gacgacgccc ggcgcccaga agcgatccat catgaagccc
      361 gcgaacacgc ggccgaacat gatgctgacg ccgaccgcgc cggcgatctt gccggcgtcg
      421 agcgcccgaa agccgcggtc ctcgagcaac gggacgaggt tcgtgatcgt gccgccgata
      481 ccggttgcga cgagcagaat ggaaacgatc agtacccaga aacggtaatt gcgcactgct
      541 gtgccgagcg tgacgccgcc ctccgcgcgg tgtgtcacga ccccgtcatc gctcacagcg
      601 ctgggcgctg gcgggtcgcg aaaccaggcg atggcgatcg gcagcgcgac ggcgagcggc
      661 agcagcgaga tgcccaggaa ggccactcgc cagccgaaat tcccgatcag ccagatcgtc
      721 agggttggaa ggaatgccgc ggtaatgccc gtgccgagga gagtaattgc gagcgcgagg
      781 ccccggttct cgacgaacca gccgttgatc gcgcgtgtcc acgtgacggg ggtcgatccg
      841 ccgccagcga aacccgtgac gaaccacatg gcatagaaga cggcgatcga cgccggcgtc
      901 aacgcgagcg cagcgaagct cagtccgaaa agagtcagcg taaggatcgc catgcgccgc
      961 acgccgtagc gatctgcgag cgcgccgacc atcggcacga ccagcacgac ggcaatggtg
     1021 aaccagagga tcgcgagctg gaactcacca cggccccagc cgaactcttc ggtgatgggc
     1081 cgcgtgaagg gaccgagcga attgtagggg atcggtgagg caccgaaggc gacgccgagc
     1141 gaggaggccg ccaacaccgc ccagccgcgc cggaactcag caaaggatcg ggtcatggca
     1201 tgaaattgat ggccgcacga gcgcactgtg ggtcgaggtc gagcgtccgc gcaaggagaa
     1261 tccgccagcg acgccgcttg gcggcgggag tgagccgggc attcgcggga cttgtcgagg
     1321 ggagagtcac gaactcgagg ggcgcgacgt gccggggcgg aattccgggc agcacgaggc
     1381 gcgtgaagag gcggtgggcc actccgccat tgaatgcgat gactgcgatc gagcggtggc
     1441 gcagcaacaa ttggtggatg ccgttggccc gcgcagtcgc gggttctatg gcggcatcgg
     1501 cgcttcccgg acgcgtcgct tcagcgagca catcccagac gcatacgccg acagcccgga
     1561 gggctgcgac gcgtcgcgcg taaggggcgt ccgccggcac gccgaacagc gcctgcatga
     1621 tcggccagaa ctggttgcgc gggtgcgcgt agtactcgcg tgcacgcagg gatgcgatgc
     1681 cgggcaggct gccgaggatc aggagtcgtg gctttgcgcc gaggatgggt gcaaacgcgt
     1741 gaacccgcac tggcctagat cgtgatgaat tcattgaacc agaccatggc ggcaaaaccg
     1801 gcgaggagac tcgcggtggc gccgcccgcc atgccacggc gatgcgtctc cggaatcacc
     1861 tctgcgctga tgacgtagag catcgcaccg gccgcgaatg cgagcgccca cggcaggagg
     1921 ccaccagcaa gcccgacggc cgcggcgccg acaatgccgc cgcccgtctc gatgagtccg
     1981 gtgagcacgc cgagcccgaa actgcgcgca cggccgaagc cgcccgacaa catcgccatt
     2041 gcgacgacaa ggccctccgg gatgttctgg atggcgatcg cggtggcgat gcgcggcgtc
     2101 agggtggggt cttgcgcgcc gatcgtcacg ccgacactga gcccctccgg aaagttgtgc
     2161 agggcaatcg ccgtaacgag gagaaaggac atcgagtgag ggcggtccgc tccgtcatgg
     2221 cccttgaact ggtgctcgtg ggggaaatgc cggttggccc acgagacgca ccaggcaccg
     2281 acgcccagcg caccgagcgt gagcggcgtc gcccatgcgc cgggcaccct ctgcgcggct
     2341 tcgtgcagcg ctgcgggcag cagcgcgtag acgctcgctg cgagcatcac gcctgcagca
     2401 agggagatga aatgattcgc cagacgcggc gacagctccc gcagtgcgag gacgggcgcc
     2461 gcgccgagcg ccgtcgccac cgtgccgctc acgaacacga tccagagcgc ggaccagaag
     2521 ctcattgcca tctccccatt gtgatcgccc attatggggt gcgaggcttg gcacccaggt
     2581 cggaattggc tagtttccgc gccatgatcc gcatcaacgt caacggagtg caccacacgg
     2641 tcgacgtgga gccggatact ccgctcctct gggtattgcg cgacgcgctc gggctcaccg
     2701 gcaccaagta cggctgtggt atcgcccagt gcggcacctg taccgtacac gtcggcggcc
     2761 agcccacgcg ggcctgcgtg ctgccggtag gcagtgtcgg cgacatgcgg atcaccacga
     2821 tcgaagggct gcatcctgag ggccggcatg cgctgcagct ggcgtggatt gccgagcagg
     2881 taccgcagtg cggctactgc cagtccggcc agatcatgag cgcggcggcg ctgctggctg
     2941 ccacgcctca accggccgat gcggacatcg acgccgccat ggcggggaac ctgtgccgtt
     3001 gcggcaccta cccacgcatt cggaacgcga tcaagcgcgc ggccggactg gcaggcgcat
     3061 cgaagccacg gacggtgaag ggagcgtcgt gagtcttgcc cggcgtgaat tcctcgcgct
     3121 ttcgggcgcc ttgtgtgtcg gcgtcgcgct tccgcgagcg gctggcgcgg taaagcccct
     3181 cgcggcgggg aaagcgatac cgctgacagc ctacgtcgtc atcgagccag gcggcctcgt
     3241 gcgtgtcctc tcgcccgtgt cggaaatggg gcagggcacg ttcgctgcgc atgccgcgat
     3301 cgtcgcggac gaactggggg tcgactattc gatggtgcgc gtcgagaccg cgatgcccgc
     3361 cgatccgtac cggcatcctg gtccgggcgg agcgccagcc agccagagta cgggcggcag
     3421 ctggggtgta cggcgctggt atggtccgct gcgcaagggt gccgcacagg cgcgcgaaat
     3481 gctgatcgct gtagcagccg ccaggtccgg gcttgctgca gcggagtttg ccgtgagcga
     3541 cggtgtcgtg aagcacgcga cttcggcggc tgcgcgctat acgtttggcg aactggccgc
     3601 ggatgcggcg aagctcccac cacccgctga accgctgctg cggccccgct ccgcgcgccg
     3661 ccatgttggc gcgcgcctcg agcgacccga cattcccgac aaggtgcgtg gccgcaccct
     3721 ctacagcagc gattttcgtc gaccggggat ggtgtacgca tgcgcgcgca tcgcccctgt
     3781 ccagggcgca aaacccacgc gcatcgatcg ggcatcggcg ctcgccgtga agggcgtgat
     3841 cgaggtcgtc gcgctgccga acggggcggc ggtcgtagcc tcgtcgcagt gggcggccat
     3901 gcagggcgcg cgtgcgctcg tgatcgagtt tgagcgcacc gcaaacgatt ccctgaattc
     3961 ggcagcagtg agtgccgcga tgcgcgaggg gctcggcgcc gtgtcgcggg cggtgctgcg
     4021 cagcgaaggc gacttcgagg tcgcgctcgc gagcgcggcc cgggtcgtga cagccgacta
     4081 tgaagtcccc tatctcgcca atgcgccgct tgagccctgg agctgcacgg ccgagatcgt
     4141 cgctgacggc tcgctcgatc tctggggtcc gttccaggcg caggatcgcg cgcgaaccgg
     4201 tgccgcgcgg acgctcggca tcgatggcgt gcgcgtgcat acgttgccgc tcggcggcgg
     4261 attcgggcgc cgtctgaacg atgatggcgt gccggctgcc gccctcgtcg cgagggcgat
     4321 gggcaagccc gtcaagctct tctggacccg ggaaaccgag ttcgcggcgg gtttcggccg
     4381 gccggcgcag atggcccgca tcaccgcagc gctcgacctg tccggccggg tgacggggct
     4441 gcatttccgg gcttcgggcc cgtcattgat cctgcacttc gcaccgggtc cgccggttgt
     4501 gccggatgcg acgtttctcg acggcacgtc ggtccagaac ctcgatgtcg tgcgctatgc
     4561 gttcggcgcc tatcgcgtcg attacgcgct gcgccacaat cactttccga ccggcccgtg
     4621 gcgatccgtt ggcgcgacgc agaacgcgtt cttccttgag tccttcatcg acgagatcgc
     4681 tcatgctacg gcgcgggatc cggtgcagtt gcgtcgcgaa ctgctggcgc acgatccgcg
     4741 cgcgctgcgc gtgattgacg aggctgcaag gcgcggcggc tggggcacgc cggtcaagcc
     4801 cggccacgcg ctcggttttg cgtacttcga atgctacggc agtctctgcg cgcatgttgc
     4861 ggaggtgtca atcaaggacg gcgccccgcg cgtgcaccgc gtggtctgcg tgctcgattg
     4921 cggcgaagtc gtgacgccgg acggcgcacg cgcgcaggcc gaaggcggca tcgtgcaggg
     4981 gctgtcagcg gcgctcggtg aggcgcttac gctgaagggc ggcgccgcga tcgagcagaa
     5041 cttcgacacc tatccgttgc tgcgcctgcc cgacgcgccg cgcgagatcg acgtgcattt
     5101 cattcagtcg ggcgagccgc tcggcggcgt cggcgagccg gtgctgccgc cgatcgcgcc
     5161 agcggtcgcg aacgcggtgc gcgtgctcac gggcaagccg gtgcggcggt tgccaatcct
     5221 ggtgccgggt tttcggttat tcggttattc gc
//
LOCUS       DAORUQ010000203         5233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19252_length_5233_cov_5.891271, whole genome shotgun sequence.
ACCESSION   DAORUQ010000203 DAORUQ010000000
VERSION     DAORUQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19252_length_5233_cov_5.891271"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="eno"
                     /locus_tag="PLW13_13850"
     CDS             <1..723
                     /gene="eno"
                     /locus_tag="PLW13_13850"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252325.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQY71514.1"
                     /translation="HALKKVLHGQGLNTAVGDEGGFAPNLPSNEAAIEAIITATGKAG
                     YRMGQDVTLALDCAASEFYRDGRYKLAGEGKEFDAAGFAAYLDALAQRYPILSIEDGM
                     DESDWDGWAELTRLAGARVQLVGDDLFVTNTKILATGIERRIANSILIKFNQIGSLSE
                     TLDAIAMARKAGYTAVISHRSGETEDTTIADLAVGTAAGQIKTGSLCRSDRVAKYNRL
                     LRIEAELGAQAPYRGRAELSRG"
     gene            780..1061
                     /locus_tag="PLW13_13855"
     CDS             780..1061
                     /locus_tag="PLW13_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQY71515.1"
                     /translation="MISWNWVCAGLGLLLLVLQVMLWTGQGSVAEVVALQRQIRSFAS
                     ANEELRERNRRLEVEVAELKNGLDSVEEMAREELGLVRQGETFYVLIKR"
     gene            1113..1811
                     /gene="ispD"
                     /locus_tag="PLW13_13860"
     CDS             1113..1811
                     /gene="ispD"
                     /locus_tag="PLW13_13860"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQY71516.1"
                     /translation="MVWGAVPAAGSGSRMGSSVPKQYLEIAGARLAEHTLGALLAAGC
                     IHEIVVAIAPDDALWPTLPEALRARVRTVHGGDERAASVLNALDGFSQRPAPGDWVLV
                     HDMARPCVRPERIRAMIDELGRDEVGGLLALPLVDTLKRADGAQRVRETVDRSALWRA
                     QTPQVFRFGVLERALREARASGVTITDEAMAVERLGLAPRLLTGSADNIKVTIADDME
                     LADYYLSRRRGKPA"
     gene            1808..2302
                     /gene="ispF"
                     /locus_tag="PLW13_13865"
     CDS             1808..2302
                     /gene="ispF"
                     /locus_tag="PLW13_13865"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQY71517.1"
                     /translation="MRIGHGYDVHRFGPGEAVMLGGVRIAHTHGLVAHSDGDVALHAL
                     CDALLGAIAAGDIGRHFPDSEPRFKGADSRELLRACMALVRREGYRLGNADITVVAER
                     PRVAPHVGAMRTNIAADLGAGVHQVNVKATTTEGLGFTGRREGIAAHAVVLLEPLPAG
                     ERGA"
     gene            2326..2961
                     /locus_tag="PLW13_13870"
     CDS             2326..2961
                     /locus_tag="PLW13_13870"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQY71518.1"
                     /translation="MTSQRTRARLVERLFEQGITDIRVLEVMRTTPRHLFLDEALAHR
                     AYEDASLPIGFNQTLSQPYIVARMTGLLLAGGGMKRVLEVGTGSGYQTAVLAQLVEEV
                     YSIERIRPLQERARLRLRELKLHNVTLRHGDGFEGWAAKAPFDGILTTAAPRAVPQEL
                     VAQLRVGGRLVIPVGDDRKQQLSVVTRTETGFEVREVEPARFVPLLSGMVR"
     gene            3307..4194
                     /locus_tag="PLW13_13875"
     CDS             3307..4194
                     /locus_tag="PLW13_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY71519.1"
                     /translation="MIRSPSITVVIVTCLLAGLAGCASRPYYSDLPEPGARAPVAHAA
                     AAHPGVNTYTVKPGDTLHSIAFAAGTDWQTLASLNRIASPYTIYPDQQLKLSASSVPV
                     TLGMDPDVPAPAPVRAQSAPDAGAPLVPGGAAATPRAQPPVLAPAQPSQSAPPRVAAA
                     APVAPRPLPPPSPGGWQWPGAGKLVLGYANTAQPHKGIDLAGNTGDPVLAAKGGTVVY
                     AGNGVRGYGNLLIVKHDALFLSAYAHNSKLLVKEGDVVKGGQQIAEMGDSGTDRTKLH
                     FEVRKQGNPVDPLQVLPRR"
     gene            4233..5231
                     /gene="rpoS"
                     /locus_tag="PLW13_13880"
     CDS             4233..5231
                     /gene="rpoS"
                     /locus_tag="PLW13_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HQY71520.1"
                     /translation="MGLEASVQFEDEALIAAEAEFGADLAEEAEGLDAVDADEREDEF
                     IAARREASGVQRTLDATQMYLSEIGFSPLLTAEEERHYARLALKGDEAGRKRMIESNL
                     RLVVKISRRYLNRGLSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERA
                     IMNQTRTIRLPIHVVKELNVYLRAARELTQKLDHEPTAEEIAELVDKPVEDVKRMLGL
                     NERVTSVDVPLGPDSDRSLLDTIADTQVSDPAELLQDEDMRESIAAWLEELSDKQREV
                     IARRFGLAGYEGATLEEVGREIGLTRERVRQIQVEALRRLREILERQGLTGEAVFS"
BASE COUNT          871 a         1709 c         1875 g          778 t
ORIGIN      
        1 catgcgctca agaaagtgct gcatggtcag ggactcaaca ccgcggtggg tgacgagggc
       61 ggcttcgcgc cgaacctgcc ctcgaacgag gcggcgatcg aggcaatcat cacggcgacc
      121 ggtaaggccg gctaccgcat gggccaggat gtgacgctgg cgctcgactg cgcggcctcg
      181 gagttctacc gcgacgggcg ctacaagctc gcgggcgagg gcaaggagtt cgacgcggcc
      241 ggtttcgcgg cctacctcga cgcgctggcg cagcgctacc cgatcctctc gatcgaggac
      301 ggcatggacg agagcgactg ggacggctgg gcggagctca cgcgccttgc cggagcccgc
      361 gtgcagctgg tcggcgacga cctgttcgtg accaacacga agatcctcgc caccggcatc
      421 gagcggcgca tcgccaactc gatcctgatc aagttcaacc agatcggcag cctcagcgag
      481 acgctcgatg cgatcgcgat ggcgcgcaag gccggctaca ccgctgtgat ctcgcaccgc
      541 tccggcgaga ccgaggacac caccatcgcc gacctcgcgg tgggcactgc ggccggccag
      601 atcaagaccg gctcgctgtg ccgctccgac cgcgtggcca agtacaaccg cctgctgcgc
      661 atcgaggcgg agctcggcgc ccaggcgccg taccgcggcc gcgcggaact gagtcgcggc
      721 tgaggcgcac ccgggcccac ggtttcggct acgccacgat gctcggctag aatcaacgca
      781 tgatttcctg gaactgggtc tgtgcgggtc tcgggctgct gctgctcgtg ctgcaggtaa
      841 tgctgtggac cggccagggc agcgtggccg aggtcgtggc gctgcagcgg cagatacgca
      901 gcttcgcgag cgccaacgag gaattgcgcg agcgcaaccg tcgcctcgag gtggaggtcg
      961 ccgagctgaa gaacggcctc gacagcgtcg aggagatggc gcgcgaggag ctcgggctgg
     1021 tgcgccaggg cgagactttc tacgtgctga tcaagcgctg attcgtgggc gtgcagccgg
     1081 aagggcgccg cggcgccgac ggtgcgcgac ccctggtgtg gggcgcggtg cctgccgcgg
     1141 gcagcggttc gcgcatgggt tcctccgtcc ccaagcaata cctcgagatt gccggcgccc
     1201 ggctcgccga gcacacgctc ggggccttgc tggcggccgg atgcatccat gagatcgtgg
     1261 tggcgattgc gcccgatgac gccttgtggc ccacgctgcc cgaggcgctg cgggcgcgcg
     1321 tgcgcaccgt gcacggcggc gacgagcgcg cggcctcggt gctgaatgcg ctggatggct
     1381 tctcgcaacg ccctgcgccc ggtgactggg tgctggtgca cgacatggcg cgtccctgcg
     1441 taaggccgga gcgtatccgc gcgatgatcg atgagctcgg ccgcgacgag gtgggcggcc
     1501 tgctcgccct gccgctcgtg gatacgctca agcgcgccga tggcgcgcag cgcgtgcgcg
     1561 agacggtgga tcgcagcgcg ctgtggcgcg cgcagacacc gcaggtgttc cgcttcgggg
     1621 tgctggaacg ggcgctgcgc gaggcgcgcg caagtggcgt gacgatcacc gacgaggcca
     1681 tggcggtgga acgcctcggg cttgcgccgc ggctgctgac gggaagtgcc gacaacatca
     1741 aggtgacgat cgcggacgat atggaactgg cggattacta tctttcgcgc cgtcgcggga
     1801 aaccggcatg aggatcgggc acggctatga cgtgcaccgc ttcgggccgg gcgaggcggt
     1861 gatgctcggc ggcgtgcgca tcgcgcacac gcacgggctc gtcgcgcatt cggacggcga
     1921 cgtggcgctg cacgcgctgt gcgacgccct gctcggcgcc atcgccgcgg gcgacatcgg
     1981 caggcatttc cccgactccg agccgcgctt caagggcgcc gacagccgcg aactgctgcg
     2041 cgcctgcatg gcgctggtgc gccgggaggg ctaccggctg ggcaatgccg acatcaccgt
     2101 cgtcgcggag cgaccgcgcg tcgcgcccca cgtcggggcg atgcgcacca atatcgccgc
     2161 cgacttgggt gccggcgtgc accaggtgaa cgtgaaggcc accaccaccg agggcctggg
     2221 cttcacgggc aggcgcgagg gcatcgccgc gcacgcggtg gtgctgctcg agccgctgcc
     2281 agccggggag cgcggcgcgt gaagcccaac gtcaacggca tcgggatgac ctcgcagcgc
     2341 acgcgcgcgc gcctggtcga gcggctgttc gagcagggca tcacggatat ccgcgtgctg
     2401 gaggtgatgc gcaccacgcc gcgccacctg ttcctcgacg aggcgctggc gcaccgcgcc
     2461 tacgaggacg cctcgctgcc gatcggcttc aaccagacgc tgtcgcagcc gtatatcgtc
     2521 gcccgcatga ccgggctgct gctcgccggc ggcggcatga agcgggtgct ggaggtcggg
     2581 accggctcgg gctaccagac cgcggtgctg gcgcagctgg tcgaggaggt gtactcgatc
     2641 gagcgcatcc ggccgctgca ggagcgcgcg cgcttgcgcc tgcgcgaact caagctgcac
     2701 aacgtcacgc tgcgccacgg cgacggcttc gagggctggg ccgccaaagc gcccttcgac
     2761 ggcatcctca ccacggccgc gccgcgcgcg gtgccgcagg agctggtcgc acaactgcgc
     2821 gtgggcgggc gcctggtgat tccggtcggc gacgaccgca agcagcagct gagcgtggtg
     2881 acccgcaccg agacgggttt cgaggtgcgc gaggtcgaac cggcgcgttt cgtcccgttg
     2941 ttgagcggca tggtgcgctg agggcgccgg ccgcgggcag cccgaccagc gctcgaggct
     3001 gcaatcccgg atccgccgga ttcccgggtg aatcgcgatt ttcccgttgt ttcacaaggt
     3061 tttagcgcgc gtcggcgtgg gggcgctctg ccagcttttc cagtcgcttg tgaaaaatta
     3121 ccggtgttcg cggcgaatat tattgacaac gaaatgtttc gggcgcaaat ttgccccatc
     3181 taaacctcaa taaaatcata tcgataaaca ggtttgttaa acagaacgcg cacttaaagc
     3241 gcgcaacaga ctaaacgagg ggcgatttgt atgattgcgc ccgggacaca ctagctgcag
     3301 agatgaatga tccggtctcc ctctataacc gttgtgatcg tgacctgcct cctggcgggt
     3361 ctggccggtt gcgcgagccg tccctattac tcggacctgc ccgaaccggg cgcccgggcg
     3421 ccggttgcgc acgccgccgc agcccatccg ggcgtgaaca cctacacggt gaagccgggc
     3481 gataccctgc attcgattgc ctttgcggcg ggtacggact ggcagacgct ggcctcgctg
     3541 aaccgcatcg cgtcaccgta caccatctat ccagaccagc agctgaagct gagcgcctcg
     3601 agcgtgccgg tcacgctggg catggatccg gacgtgcccg cccccgcgcc ggtgcgcgcg
     3661 cagtcggcgc cggatgcggg cgcgccgctc gtgccgggcg gcgccgcggc gacgccgcgg
     3721 gcgcagccgc cggtgctcgc gccggcgcag ccgtcgcaat ccgcaccgcc ccgggtcgcc
     3781 gcagcggcgc ccgttgcgcc gcgtccgctc ccgccgccga gtcccggcgg ctggcaatgg
     3841 ccgggcgcgg gcaaactggt gctcggctat gcgaacacgg cacagccgca caagggtata
     3901 gatcttgcag gcaataccgg ggatccggtg cttgcggcca agggtggcac ggtggtctac
     3961 gcgggcaatg gagtgcgcgg gtacggcaac ctgctgatcg tgaagcacga tgcgctgttt
     4021 ctgagcgcat atgcccacaa cagcaagttg ctggtcaagg aaggcgatgt ggtcaagggc
     4081 gggcagcaga tcgcggagat gggcgacagc ggcactgacc ggacgaagct gcacttcgag
     4141 gtgcgaaagc agggcaatcc ggtcgatccg ttgcaggtac tgcccagaag atgagccggc
     4201 gacacagaaa atcacagcaa ggggacaagg caatgggact agaggcaagc gtacagtttg
     4261 aagacgaagc cctcatcgcg gccgaggcag agttcggcgc cgacctggcg gaggaggccg
     4321 agggtctcga tgccgtcgat gcggacgagc gcgaggacga gttcatcgcg gcccggcgcg
     4381 aggcatcggg cgtgcagcgc acgctcgatg cgacccagat gtacctcagc gagatcggct
     4441 tctcgccgct gctgacggcc gaggaggaac gccattacgc gcgcctcgcg ctgaaaggcg
     4501 acgaggccgg gcgcaagcgc atgatcgaga gcaacctgcg tctcgtcgtg aagatctcgc
     4561 gccgctacct caaccgcggc ctgtcgttgc tcgacctgat cgaggagggc aacctcggcc
     4621 tgatccgcgc ggtggagaag ttcgaccccg agcgcggttt ccgcttctcg acctacgcga
     4681 cgtggtggat ccgccagacc atcgagcgcg cgatcatgaa ccagacgcgc accatccgcc
     4741 tgccgatcca cgtggtgaag gaactgaacg tctacctgcg cgcggcgcgc gagctcaccc
     4801 agaaactcga tcacgagccc acggccgagg agatcgccga gctcgtcgac aagccggtcg
     4861 aggacgtcaa gcgcatgctc ggcctgaacg agcgcgtgac ctcggtcgat gtgccgctcg
     4921 gcccggattc ggaccgctcg ctgctcgata ccatcgccga cacgcaggtg tcggacccgg
     4981 cggaactgct gcaggacgag gacatgcgcg agagcatcgc ggcatggctg gaggagctga
     5041 gcgacaagca gcgcgaggtg attgcgcgtc gcttcgggct cgccggctac gagggcgcga
     5101 cgctggagga agtgggccgc gagatcgggc tcacgcgcga gcgcgtgcgc cagatccagg
     5161 tcgaggcgct gcgccgcctg cgcgaaatac tcgagcgcca ggggctcacg ggcgaggccg
     5221 ttttcagctg agt
//
LOCUS       DAORUQ010000204         5184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19561_length_5184_cov_5.592123, whole genome shotgun sequence.
ACCESSION   DAORUQ010000204 DAORUQ010000000
VERSION     DAORUQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19561_length_5184_cov_5.592123"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1535
                     /locus_tag="PLW13_13885"
     CDS             201..1535
                     /locus_tag="PLW13_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71521.1"
                     /translation="MDWLLALLALFGVISMYCVFRPRNARVRSAPWASFAFAVHTTEL
                     PWMRLALQVPLTLLLVLCGALDSMLGVIALLLVMASWLGVLWCLRRSLDSAGPVEDAL
                     QAGLGADYRQAIAPRLATGLRAAPVFADWRHPFAMKRPGVEVIRGISYAPGGVRQKLD
                     IYRPAVIPPQGCPVLLQIHGGAWVIGHKAQQALPLMWLLASKGWICVSVNYRLSPSVG
                     FPTHLEDCKRALAWVRRHGAGYGMDTGFVAVTGGSAGGHLAALMGLTGNRPELQRDDP
                     GVDTSVQACVPFYGVYDFLTRYDQHPNGAVIRGFLEDRVMHATPEQDPALWDLASPVA
                     QIHEDAPPFMLLHGELDSLAPIADARTFVQRLRRSSRNPVVFVELPGAEHGFDFMHTP
                     RTENAIDGVHRFLEWVRSRHEEALAAQPSAAAGEGAARTSLTAVNARSSEPA"
     gene            1614..2054
                     /locus_tag="PLW13_13890"
     CDS             1614..2054
                     /locus_tag="PLW13_13890"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HQY71522.1"
                     /translation="MGKIMIVVASHDAARIFEADAGACATLLEREDLLNPSGRRHRGD
                     LESDRQGSFSGVAGGAGHALGNEQGQKEEALARYVRSLAVVVNAAHHTGHCERIYLVA
                     DPHVLGALREDLDAGTRAAVAGEVVKNLVKHTVAEIRQHLPERL"
     gene            complement(2070..2345)
                     /locus_tag="PLW13_13895"
     CDS             complement(2070..2345)
                     /locus_tag="PLW13_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY71523.1"
                     /translation="MRHDGDTLHLEGPVTMETVPALLTAARAACRNGATRIDFTTVTE
                     ADSAAIALALELKRDALERQAALSFLNLPPGLHKLVELYDVGDHLAG"
     gene            complement(2348..2992)
                     /locus_tag="PLW13_13900"
     CDS             complement(2348..2992)
                     /locus_tag="PLW13_13900"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQY71524.1"
                     /translation="MRNHTTHSPARRVAALWGMLMALMLALPATAATEEPDALVKRVS
                     EEVLQILKENQDNPGGAKVQALVKEKVLGHFDFARMAALAAGLSWRKATPEQKTELTE
                     QFRTLLVRTYASALGKFKGQTLEFDPQRKSEDQRKAIVRSRLLQPGVEAITVDYKMVA
                     ASSGWKVYDVSVDGVSLVTTYRDAFAEEARANGVDGLIRMLTDKNAALASDKAT"
     gene            complement(2997..3494)
                     /gene="mlaD"
                     /locus_tag="PLW13_13905"
     CDS             complement(2997..3494)
                     /gene="mlaD"
                     /locus_tag="PLW13_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HQY71525.1"
                     /translation="MNRTTIDLWVGIFVAMGAAALVFLSLQVASMTSGTRGETYVLNA
                     RFDDLGGLKVKAPVKSAGVVVGRVADVRFDNETFEAVVSFRVEQRYQFPTDTSAKILT
                     SGLLGDQYIGLIPGGEMDNLQDGDTLEITQGALVLESLISKFLFSSTSGGENKSDDAK
                     KDDEE"
     gene            complement(3501..4301)
                     /gene="mlaE"
                     /locus_tag="PLW13_13910"
     CDS             complement(3501..4301)
                     /gene="mlaE"
                     /locus_tag="PLW13_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HQY71526.1"
                     /translation="MTKPTSFPVLRAIGARTIDSLWRLGFATRFLLATLRHTPEAFRR
                     FGLTVREIYFTGVLSLTIILVSGLFVGMVLGLQGYDTLSRFGSSEALGVLVALSLVRE
                     LGPVVAALLFASRAGSAITAEIGLMKATEQLEAMEMMAVDPIARVVAPRFWGGVISMP
                     LLGALFTATGIIGGYLVGVVMIGVDPGQFWSQMQAAVDVRDDVFNGVIKSFAFGIAVT
                     WIAVFEGYEAPPTAEGVSRATTRTVVTSALVILALDFVLTAFMFHGIR"
     gene            complement(4298..5110)
                     /locus_tag="PLW13_13915"
     CDS             complement(4298..5110)
                     /locus_tag="PLW13_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY71527.1"
                     /translation="MSNENVVEIENLSFSYSSGRQVLSKINLQIPRGRVIGIMGQSGC
                     GKTTLLRMIGGALKPSQGEVRVFGQAISQLDQKQIYAMRRRMSMLFQFGALFTDMSSF
                     ENVAFQMREHTKLEESMIRDLALMKLHAVGLRGAAHLMPSELSGGMARRVALARAIAL
                     DPELMMFDEPFAGLDPISLSVIGQLIRKLNDALGATSIIVTHDIQESLKIVDYIYFVA
                     DGRVIAEGSAEEVKASLEPYVHQFVWGEADGPVPFHYPAADYVADLLRGKRA"
BASE COUNT          828 a         1704 c         1778 g          874 t
ORIGIN      
        1 gcgcgccacg gcgagccggc gcattacgtg atcgagcgcg ccttcgtcga gtaggagcgg
       61 gccatgcccg cgatccagcg cgatacgacg cccgaacccg gcgcgcagcg ttctttcccg
      121 cccccgatgc gtgcataatc ccgcacgccg cgcccccggg gcgcggcgaa cgctgaatcg
      181 ctgcggggga aattcgcgca atggactggc tgctggcgct gctcgcgctg ttcggtgtga
      241 tctcgatgta ctgcgtgttc aggccgcgca acgcgcgggt gcgcagcgcg ccgtgggcca
      301 gcttcgcctt cgcggtgcac acgacggagc tgccctggat gcggctggcg ctgcaggttc
      361 cgctcacgct gctgctggtg ctttgcggtg cgctcgacag catgctcggc gtcatcgcgc
      421 tgctgctggt gatggcgagc tggctcggcg tgttgtggtg cctgaggcgc tcgctcgact
      481 ccgctgggcc ggtggaggac gcactgcagg ccggtctcgg cgccgattac cgccaggcga
      541 tcgcgccgcg cctggccacc gggctgcgcg ccgcgccggt cttcgccgac tggcgccatc
      601 ccttcgcgat gaagcgtccg ggggtcgagg tcattcgcgg catttcctat gcgccgggcg
      661 gcgtgcgcca gaagctcgat atctaccgtc ctgccgtgat cccgccgcaa ggctgtccgg
      721 tgctgctgca gattcacggc ggcgcctggg tgatcggtca caaggcacag caggcgctgc
      781 cgctgatgtg gctgctcgcg agcaagggct ggatctgcgt gtccgtgaat taccgcctga
      841 gccccagcgt gggcttcccg acccatctcg aggactgcaa gcgcgcgctg gcctgggtgc
      901 gccggcacgg cgccggctac ggcatggaca cgggtttcgt cgcggttacc gggggctccg
      961 ccggcgggca cctggcggcg ctgatgggcc tcacgggcaa ccgtcccgag ctgcagcgtg
     1021 acgatccggg cgtcgatacc tcggtgcagg cctgcgtgcc gttctacggc gtctacgact
     1081 tcctcacgcg ctacgaccag caccccaatg gcgccgtgat ccgcgggttc ctggaggacc
     1141 gcgtgatgca tgccacgccg gaacaggatc ccgcgctgtg ggacctggcc tcgccggtgg
     1201 cgcagatcca cgaggacgca ccgccgttca tgctcctgca cggggagctc gacagcctgg
     1261 cccccattgc cgatgcacgc accttcgtgc agcgcctgcg ccgcagctcg cgcaatccgg
     1321 tggtgttcgt ggagcttccg ggcgccgagc acggtttcga tttcatgcat acgccgcgta
     1381 ccgagaacgc catcgacgga gtgcaccgct tcctcgagtg ggttcgttcg cggcacgagg
     1441 aggcgctcgc cgcgcagcca agtgcggcgg cgggcgaggg cgccgcgagg acttcgttga
     1501 ccgcggtcaa tgccagatca tcggagcccg cctagttttg cagacatggc gcgggcacca
     1561 cgggtccagg atgcaccggc agtttcaggc aactcagcac agggagacac gggatgggca
     1621 agatcatgat cgttgtcgcg agtcacgatg ccgcgcgcat attcgaagcc gatgcggggg
     1681 catgcgcaac gctgctcgag cgcgaggatt tgctgaaccc ctcagggcgg cgccaccggg
     1741 gcgacctgga gagcgatcgc caggggagct tcagcggcgt ggccggcggt gccggccacg
     1801 ccctgggtaa cgaacagggc cagaaggagg aagcgctggc gcgctacgtg cgctcgctgg
     1861 cggtggtggt caacgcggcg caccacaccg ggcactgcga gcggatctac ctggtggcag
     1921 acccgcacgt gctgggtgcg ttgcgcgagg atctcgatgc gggcacccgc gcagccgtgg
     1981 cgggcgaagt ggtgaaaaac ctggtgaagc acacggttgc cgagatccgc cagcacctgc
     2041 ccgagcggct gtgaatcctg cagcggcact cagccggcca gatgatcgcc gacgtcgtag
     2101 agctcgacca gcttgtgcag tccgggcggc aggttgagaa aggaaagagc ggcctggcgc
     2161 tcgagcgcgt cgcgcttgag ctccagcgcc agcgcgatcg ccgcggagtc ggcctcggtc
     2221 acggttgtga agtcgatgcg cgtggcgccg ttgcgacagg ccgcgcgggc tgcggtgagc
     2281 agcgcgggca cggtttccat cgtcaccggc ccctcgaggt gcagggtatc gccgtcgtgg
     2341 cgcatgctca ggtcgccttg tcgctggcca gcgcggcgtt cttgtcggtc agcatcctga
     2401 tcaggccgtc gacgccgttg gcgcgcgctt cttcggcgaa ggcatcgcgg taggtggtca
     2461 ccaggctgac gccatcgacc gagacgtcgt agaccttcca gccgctgctg gcggctacca
     2521 tcttgtagtc gaccgtgatc gcctccacac ccggctgcag cagccgcgag cgcacgatcg
     2581 ccttgcgctg gtcctcggac ttgcgctgcg gatcgaactc gagcgtctgg cccttgaact
     2641 tgccgagcgc cgaggcgtag gtgcgcacca gcaaggtgcg gaactgctcc gtcagttcgg
     2701 tcttctgctc cggcgttgcc ttgcgccagc tcagtccggc cgcgagcgcg gccatgcgcg
     2761 cgaaatcgaa atgccccagc accttttcct tgaccagtgc ctggaccttg gcaccaccgg
     2821 ggttgtcctg gttttccttg aggatctgca gcacttcctc gctgacgcgc ttgaccagcg
     2881 catcgggttc ctcggtggcg gcggttgcgg gcagcgccag catcagcgcc atcagcatgc
     2941 cccagagcgc agcgacgcgc cgcgccgggg agtgtgtcgt gtggttgcgc atgggcttac
     3001 tcctcgtcgt ccttcttcgc gtcatcgctt ttgttctcgc cgccggaggt cgagctgaac
     3061 aggaacttcg agatcaggct ctcgagcacc agggcgccct gggtgatctc cagggtgtcg
     3121 ccgtcctgca gattgtccat ctcgccgccg ggaatcaggc cgatgtactg gtcgcccagc
     3181 agcccggagg tcaggatctt ggccgaggtg tccgtgggaa actggtagcg ctgctcgacg
     3241 cgaaagctca cgacggcttc gaaggtctcg ttgtcgaacc tgacgtcggc cactcggccg
     3301 accaccacgc cggcgctctt gaccggtgcc ttgaccttga ggcccccgag gtcgtcgaag
     3361 cgggcattga ggacgtaagt ctcgccgcgc gtgccactgg tcatgctcgc gacctggagc
     3421 gacaggaaca ccagggccgc ggcgcccatg gccacgaata tgcctaccca gagatcaatg
     3481 gtcgtgcggt tcatagcgtt ctagcgtatc ccgtgaaaca tgaatgcagt gagtacgaaa
     3541 tccagcgcca ggatgaccag cgcggaagtc acgacggtgc gcgtggtggc cctggagacc
     3601 ccctcggcgg tcggcggggc ctcgtagccc tcgaacaccg cgatccaggt caccgcgatg
     3661 ccgaacgcga agctcttgat cacgccgttg aacacgtcat cgcgtacgtc caccgcagcc
     3721 tgcatctgcg accagaactg gcccgggtcg acgccgatca tcaccacgcc gaccaggtag
     3781 ccgccgatga tgccggtggc ggtgaacagc gcgcccagca gcggcatcga aatcacaccg
     3841 ccccagaagc gcggtgcgac cacgcgcgcg atcggatcga cggccatcat ctccatggcc
     3901 tcgagctgct cggtcgcctt catcaggccg atctcggccg tgatcgcgct accggcgcgg
     3961 ctggcgaaca gcagggcggc caccacaggg ccgagttcgc gcaccagcga taatgccacc
     4021 agcacgccga gggcttccga ggagccgaag cgggagaggg tgtcgtagcc ctgcaggccg
     4081 agcaccatgc cgacgaagag tcccgagacc aggatgatcg tgagcgacag cacgccggtg
     4141 aagtagatct cgcgcaccgt cagcccgaaa cggcggaacg cttccggggt gtgccgcagc
     4201 gtggccagca ggaatcgggt ggcaaagccc aggcgccaga gcgaatcgat ggtgcgggcg
     4261 ccgatcgcgc gcaggacggg gaacgaggtc ggtttcgtca tgcgcgcttg ccccgcagca
     4321 ggtccgcgac gtagtccgcg gcagggtagt ggaagggcac ggggccgtcc gcctcgcccc
     4381 agacgaactg gtgcacgtag ggctccagcg aggccttgac ctcctcggcg ctgccttcgg
     4441 cgatcacgcg gccgtcggcg acgaaataga tgtagtccac gatcttcagc gattcctgga
     4501 tgtcgtgcgt cacgatgatc gaggtcgcac cgagggcgtc gttgagcttg cggatcagct
     4561 ggccgatcac ggacagcgag atcgggtcga gaccggcgaa gggctcgtcg aacatcatca
     4621 gttccgggtc gagcgcgatg gcgcgcgcca gcgccacgcg tcgcgccatg ccgcccgaga
     4681 gctccgaggg catcaggtgc gccgcgccgc gcaggcccac cgcgtgcagc ttcatcagcg
     4741 cgaggtcgcg gatcatggat tcctcgagct tcgtgtgctc gcgcatctgg aatgccacgt
     4801 tctcgaaact cgacatgtcg gtgaacagcg cgccgaactg gaacagcatg ctcatgcgcc
     4861 ggcgcatggc gtagatctgc ttctggtcga gttggctgat ggcctgcccg aacaccctta
     4921 cctcgccctg cgagggtttc agggcgccgc cgatcatgcg cagcagcgtg gtcttgccgc
     4981 agccgctctg gcccatgatg ccgatcacgc gaccgcgcgg gatctgcagg ttgatcttcg
     5041 agagcacctg gcggccggaa gaataactga acgagagatt ctctatttct acgacgtttt
     5101 cgttagacat cgacaatcgg gcctgcgggc tgaaccggcg attctatagc atcactgcgc
     5161 cgatggagtc ggtcatattc acga
//
LOCUS       DAORUQ010000205         5177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19598_length_5177_cov_6.128075, whole genome shotgun sequence.
ACCESSION   DAORUQ010000205 DAORUQ010000000
VERSION     DAORUQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19598_length_5177_cov_6.128075"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLW13_13920"
     CDS             <1..999
                     /locus_tag="PLW13_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY71528.1"
                     /translation="VTTLVLRPADTDGAGLILTEGGSGDALALYREGLFMADPFVALP
                     PGKVVALHEIIPLAELEQTELYKLCMAPGGMHDSLGADVEVDGIVEAQLRVSRARGAR
                     PFGKRETQLCTLLLPHLERAVRIYMRLHRVESERALYAGAISQLAVGTVILDARGAVS
                     ATNTLADGILAARDGLWVAEGVLRVQNPREAAELRRIVGELAESQRAGQPALAQALRI
                     SRPSGRADLGLVLRPLPATDESQGVPGIAVFVSDPEERSDAPVQVLVKLYGFTPTEAL
                     VAIQLANGLNIDDAAAELGMTRNTARAHLRSVFNKTGISRQPALVRLILKSVASLA"
     gene            1033..1788
                     /locus_tag="PLW13_13925"
     CDS             1033..1788
                     /locus_tag="PLW13_13925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008004553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71529.1"
                     /translation="MGMLDGKVALVTGAGQGVGRGIALALAGEGARIAVAGRTEGKLL
                     ETCAEIEGRGGRAVPIVCDVMIAADIERCVQQTVTALGTVDILINNAQVVPLGRLLDV
                     TEEHYQEGMDSGPLATLRFMKACYPYLRDGGCIVNLGSSSAIRWDPIGYGAYAAAKEA
                     IRALTRAAACEWGPDGIRVNCVLPLAMSPAMDGWIASRPEEAAAFFHTIPLGRVGDCE
                     TDIGRAVVFLCGPDARYITGHSLPLDGGQAFMR"
     gene            1799..2635
                     /locus_tag="PLW13_13930"
     CDS             1799..2635
                     /locus_tag="PLW13_13930"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQY71530.1"
                     /translation="MDAWPLIQALLLGVVEGLTEFLPVSSTGHQIIVADLIGFGGERA
                     KAFNIIIQLGAILAVVWEYRAKILEVVLTLPREPRSQRFTVNLLLAFLPAAVLGVAFS
                     DQIHAYLFNPLAVAVALVLGGVVMLWAERREHTVDVEEVDAMDWHHALKVGCAQCLAL
                     VPGTSRSGATIIGGLLFGLSRRAATEFSFFLAMPTMVGAAVYSGYKYRELFQAADLPV
                     FAVGFVTSFVFAMVAVRALLKFVASHSYAAFAWYRIGFGLLILATWQFALIDWSGAAA
                     AH"
     gene            2682..3587
                     /locus_tag="PLW13_13935"
     CDS             2682..3587
                     /locus_tag="PLW13_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY71531.1"
                     /translation="MKIKSLGYLWIESTDVSKWRDYGTNILGLQIAPTMPADGNIYLK
                     MDARPYRFAVCPGERDRLAFAGWELESEGAFTAALEELHAAGVAFELPGADIARQRRV
                     RAVARLQDPAGATLELYHGADLDYAKFISPQGIAEFETGFNGNMGLGHIVLPTKKLAE
                     TRRFYREVLGFADTDYMHFHFSPDPKDPGQGLHFMHVDNPRHHSLALYEDPNDSPSGC
                     VHAMVEVSEVDEVGYCLDRVNAAGIPVVSSLGRHTNDRMLSFYMATPGGFAMEFGCEG
                     LQMDWEGYTPTVSTLPSIWGHKFQM"
     assembly_gap    3590..3689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3690..4495
                     /locus_tag="PLW13_13940"
     CDS             <3690..4495
                     /locus_tag="PLW13_13940"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQY71532.1"
                     /translation="PSYYIAHGGGPWVYMDGALRAMHAPLERSLAAIPRELGCRPRAL
                     LVISAHWEAPQFTLLESARPGMLYDYGGFPPHTYNVQYPAPGAPELAARAHGLLGAAG
                     IRTAGETRRGFDHGVFVPLAVMYPDADIPVLALSLKLGLDPAEHLAAGRALAPLRAAG
                     VLIIGSGSGYHNMAGVDARAIAASAAFDAWLQQALCGASGAAREALLERWAEAPSARY
                     AHPREEHLLPLMVAAGAGGDEAGVCAWQQRDMMGGITLSSFRFGALPGG"
     gene            4571..5092
                     /locus_tag="PLW13_13945"
     CDS             4571..5092
                     /locus_tag="PLW13_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQY71533.1"
                     /translation="MLFAFASLLAPVLAFAQCPALLDQQMRPLGKQEAVSLCERYAGQ
                     PLLIVNTASRCGYTRQFKGLEALNQEFRERGLRVIGFSSDDFRQEAADEAETAKVCYV
                     NYGVTFDMYAQIHVRGEEAHPLFRELARQSEAPGWNFYKYLVDREGKVVAAFPSATAP
                     DDKALRTAIEKLL"
BASE COUNT          733 a         1743 c         1802 g          799 t
ORIGIN      
        1 gtgaccacgc tggtgttgcg ccccgccgac accgatggcg ccgggctgat cctcaccgag
       61 ggcggttccg gcgatgccct ggcgctctat cgcgaaggcc tgttcatggc cgaccccttc
      121 gtcgccctgc cgccgggcaa ggtggtggcg ctgcacgaga tcatcccgct cgccgagctc
      181 gagcagaccg agctctacaa gctgtgcatg gcgccgggcg gcatgcacga cagcctcggt
      241 gccgatgtcg aggtcgatgg catcgtcgag gcgcaactgc gggtctcgcg cgccaggggc
      301 gcccggccct tcggcaagcg cgagacgcag ctctgcacgc tgctgctgcc gcatctggag
      361 cgggccgtgc gcatctacat gcgactgcat cgcgtcgagt ccgagcgcgc gctctatgcc
      421 ggcgcgatct cgcaactcgc ggtgggcacg gtgatcctgg acgccagggg cgcggtgagc
      481 gccacgaaca ccctcgccga cgggatcctc gccgcccgcg acggcctgtg ggtggccgag
      541 ggcgtgctgc gcgtgcagaa cccgcgcgaa gcggccgaac tcaggcgcat cgtcggcgag
      601 ctcgccgaga gccagcgcgc gggccagccc gcactggcgc aggcgctgcg catctcgcgc
      661 ccgagcgggc gggccgacct cggcctggtg ctgcgtccgc tgccggcgac ggacgaatcg
      721 cagggcgtac ccggcatcgc ggtcttcgtc agcgatcccg aggagcgctc ggacgcgccg
      781 gtacaggtgc tggtgaagct ctacggtttc acgccgaccg aggcgctggt cgcgatccag
      841 ctcgccaacg gcctgaacat cgatgatgcg gccgccgagc tcggcatgac gcgcaacacc
      901 gcgcgcgcgc acctgcgctc ggtgttcaac aagaccggca tcagccgcca gcccgcgctg
      961 gtgcggctga tcctgaagag cgtggcctcg ctggcctgag atttccccga cacaacaaca
     1021 gaggaaaccg agatgggaat gctggacgga aaagtggcgc tggttaccgg agccggacag
     1081 ggcgtgggac gcggcatcgc gctcgcgctg gcgggcgagg gcgcgcggat cgcggtcgcg
     1141 ggacgcaccg agggcaagct gctcgagacc tgcgccgaga tcgaagggcg tggcgggcgc
     1201 gcggtcccga tcgtctgcga cgtgatgatc gccgccgaca tcgagcgctg cgtgcagcag
     1261 acggtcactg ccctgggcac ggtcgacatc ctgatcaaca acgcgcaggt ggtgccgctg
     1321 gggcgcctgc tcgatgtcac cgaggagcac taccaggaag gcatggactc cgggccgctc
     1381 gcgacgctgc gcttcatgaa ggcctgctat ccgtacctgc gcgatggcgg ctgcatcgtg
     1441 aacctcggct cctcgtcggc gattcgctgg gacccgatcg gctacggcgc ctatgccgcg
     1501 gccaaggaag cgatccgcgc gctcacgcgc gccgccgcct gcgagtgggg cccggacggc
     1561 atccgcgtca attgcgtgct gccgctggcc atgtcgccgg cgatggacgg ctggatcgcc
     1621 tcgcggcccg aggaggcggc ggccttcttc cacaccatcc cgctcggtcg cgtgggcgac
     1681 tgcgaaaccg acatcggccg cgccgtggtg ttcctgtgcg gccccgatgc gcgctacatc
     1741 acgggccatt cgctgccgct cgacggcggc caggccttca tgcgctgagg agaccgcgat
     1801 ggatgcatgg ccgctgatcc aggccctgct gctcggcgtg gtcgaggggc tcacggagtt
     1861 cctgccggtc tccagcaccg gccaccagat catcgtggcc gacctgatcg gattcggcgg
     1921 cgagcgcgcc aaggcgttca acatcatcat ccagctcggc gcgatcctgg cggtggtgtg
     1981 ggaataccgc gcgaagatcc tcgaggtggt gctcacgctg ccgcgcgagc cccgctcgca
     2041 gcgcttcacc gtgaacctgc tgctggcctt cctgcccgcg gccgtgctgg gcgtggcctt
     2101 ctctgaccag attcacgcct acctgttcaa cccgctggca gtggcggtgg cgctggtgct
     2161 cggcggcgtg gtgatgctct gggccgagcg gcgcgagcac accgtcgacg tcgaggaggt
     2221 ggacgcgatg gactggcatc acgcgctgaa ggtcggctgc gcccagtgcc tcgcgctggt
     2281 gccgggcacc tcgcgctcgg gggcgaccat catcggcggc ctgctgttcg ggctgtcgcg
     2341 gcgcgcggcc accgagttct cgttcttcct cgcgatgccg accatggtgg gggccgcggt
     2401 gtactcgggc tacaagtacc gcgagctgtt ccaggccgcg gacctgccgg tgttcgccgt
     2461 cggcttcgtc acctcgttcg tgttcgcgat ggtcgcggtg cgcgcgctgc tgaagttcgt
     2521 cgccagccat tcctacgcgg cgttcgcgtg gtaccgcatc ggcttcggcc tgctgatact
     2581 cgccacctgg cagttcgcgc tgatcgactg gtcgggcgcg gccgcggcgc actgagcccc
     2641 ctttctttac ctgccaaccc gacaattacc gaggagagcc aatgaagatc aagagcctgg
     2701 gttatctgtg gatcgagtcc accgacgtgt ccaagtggcg cgactacgga acgaacatcc
     2761 tggggctgca gatcgccccg acgatgccgg ccgacggcaa catttacctg aagatggacg
     2821 cgcgcccgta ccgcttcgcg gtgtgtcccg gcgagcgcga tcgcctcgcg ttcgcgggct
     2881 gggagctcga gagcgagggc gccttcaccg cggcactcga ggagctgcat gccgccggcg
     2941 tggccttcga actgcccggg gccgacatcg cgcgccagcg ccgcgtgcgc gccgtggccc
     3001 ggctgcagga tccggcgggc gcgacgctcg agctctacca cggcgcggac ctcgactacg
     3061 cgaagttcat ctcgccgcag ggcatcgcgg agttcgagac cggcttcaac ggcaacatgg
     3121 gcctcggcca catcgtgctg cccacgaaga agctcgccga gacgcggcgc ttctaccgcg
     3181 aggtgctggg cttcgccgac accgactaca tgcacttcca tttctcgccc gaccccaagg
     3241 atccgggcca gggcctgcac ttcatgcacg tcgacaaccc gcgccaccac agcctggcgc
     3301 tgtacgagga cccgaacgac agcccctcgg gctgcgtgca cgcgatggtc gaagtgagcg
     3361 aggtcgacga ggtgggctat tgcctggacc gcgtgaatgc cgcgggcatc ccggtggtgt
     3421 cctcgctcgg gcgccacacc aacgaccgca tgctgtcgtt ctacatggcc accccgggcg
     3481 gcttcgcgat ggagttcggc tgcgagggcc tgcagatgga ctgggaaggc tacacgccca
     3541 cggtgagcac gctgccgagc atctgggggc acaagttcca gatgtgaggn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcccagctac tacatcgccc acggcggcgg
     3721 gccgtgggtg tacatggacg gcgcgttgcg cgcgatgcat gcgccgctcg agcgttcgct
     3781 ggcggcgata ccgcgcgagc tcggctgccg gccgcgcgcg ctgctggtga tctcggcgca
     3841 ctgggaggcg ccgcagttca cgctgctgga gtcggcgcgg cccgggatgc tctacgacta
     3901 cggcggcttc ccgccgcaca cctataacgt gcagtatccg gcgccgggcg cgccggagct
     3961 ggcggcgcgt gcgcacgggt tgctcggcgc cgcgggcatc cgcacggcgg gcgagacgcg
     4021 ccgtggcttc gatcacggcg tgttcgtgcc gctggcggtg atgtatcccg acgccgacat
     4081 cccggtgctg gcgctgtcgc tgaagctcgg cctcgatccg gcggagcacc tcgccgcggg
     4141 gcgcgcgctg gcaccgttgc gcgccgcggg cgtgctgatc attggcagcg gttccggcta
     4201 ccacaacatg gccggcgtgg atgcgcgcgc gatagcggca tccgccgcct tcgatgcctg
     4261 gctgcagcag gcgctctgtg gtgcgtccgg cgccgcgcgc gaagcgctgc tcgaacgctg
     4321 ggcggaggcg ccctcggcgc gctacgccca tccgcgcgag gagcacctgc tgccgctgat
     4381 ggtggctgcc ggcgccggcg gcgacgaggc gggcgtgtgc gcctggcaac agcgcgacat
     4441 gatggggggc atcacgctgt cctcgttccg tttcggcgcg ctgcccggcg gctgagccgc
     4501 acggatccgc cgcggcccgc gtggcgtaga atccgctaac gaatgcccgg agactccgac
     4561 catgaaagca atgctgtttg ccttcgcctc cctgctcgcg cccgtgctgg cgttcgcgca
     4621 atgccccgcg ctgctcgacc agcagatgcg tccgctcggc aagcaggagg ccgtgagcct
     4681 gtgcgagcgc tacgccggcc agccgctgct gatcgtgaac accgcgagtc gttgcggcta
     4741 cacgcggcag ttcaaggggc tggaggcgct gaaccaggaa ttccgcgagc gcgggctgcg
     4801 cgtgatcgga ttctccagcg atgatttccg ccaggaagcc gccgacgagg ccgagaccgc
     4861 gaaggtgtgc tacgtgaact acggcgtcac cttcgacatg tacgcgcaga tccatgtgcg
     4921 cggcgaggag gcgcacccgc tgtttcgcga gctggcgcgc cagagcgagg cgccgggctg
     4981 gaatttctac aagtacctcg tcgatcgcga gggcaaggtc gtggcggcct tcccgagcgc
     5041 cacggcgccc gacgacaagg ccctgcgcac ggcgatcgag aagctgctct gatcgatcgc
     5101 cgccggcgcg tggcaaagcc tgccgttccg gtcttatgat gtgaccatga gcgaggagca
     5161 ggggagtcgc ccatgag
//
LOCUS       DAORUQ010000206         5176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19603_length_5176_cov_5.609842, whole genome shotgun sequence.
ACCESSION   DAORUQ010000206 DAORUQ010000000
VERSION     DAORUQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19603_length_5176_cov_5.609842"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PLW13_13950"
     CDS             complement(<1..1287)
                     /locus_tag="PLW13_13950"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQY71534.1"
                     /translation="MNVHLKLETLGHFINGAAVADGARTQPVFNPATGQVVREVSLAA
                     RATVEQAIAAAAAAFPAWRDTPPLKRARIMFRFKDLLEQNANAIAELITEEHGKVFDD
                     ALGEFQRGVEVVEYACAAPQLLKGEHAKNVGPAIDSWSEFHPLGVVAGITPFNFPAMV
                     PMWMFPMAIVCGNTFVLKPSERDPSAALLIARIFKEAGLPDGVFNVVNGDKEAVDTLL
                     TDPRVQAVSFVGSTPIAEYVYQTGTKHGKRIQALGGAKNHAIVMPDADIDNAVSALMG
                     AAYGSCGERCMAISVAVAVGDEVGDALVAKLKQRLAHLKVGPGTLPGMDMGPLVTRAH
                     YEKVRGYVDQGVEEGAQLVVDGRGLTVPGHEEGFFLGGCLFDRVLPSMRVYNEEIFGP
                     VLCVVRVKSQEEAMALIDAHEYGNGTCIFTRDGEAAR"
     gene            complement(1289..>2608)
                     /locus_tag="PLW13_13955"
     CDS             complement(1289..>2608)
                     /locus_tag="PLW13_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQY71535.1"
                     /translation="LPALSREELEAHWMPFTPNREFKRNPRMIVRAEGCHYVTDDGRR
                     VFDSLSGLWCCGLGHGRTEISRAVAQQVQELDYAPAFQFGHPLSFRLANMLEAMAPPG
                     LDYVFFTNSGSEAADTALKMARAYWRQKGQPTKTKFIGRAKGYHGVNFGGISLGGIGG
                     NRKLFGHTLDADHLPHTLLAANAFSRGMPENGVELADQLEELVALHDASNIAAVIVEP
                     FSGSAGVIVPPKGYLQRLRELCTKHDILLIFDEVITGFGRAGDVFGAQAFGVTPDILN
                     VAKCLTNGAVPMGAVIASGEIYRTFMAAGGAEYAVEFPHGYTYSAHPVACAAGIAALD
                     VFQREDMVGKVRALAPYFEDGLHGLKGVKHITDIRNFGLAGALQIEARPGEPLRRPYE
                     IALAMWERGFYVRWGGDTLQFAPPFVTEKADLDQLFNTLNDVIPTVN"
     assembly_gap    2609..2708
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2797..3729
                     /locus_tag="PLW13_13960"
     CDS             2797..3729
                     /locus_tag="PLW13_13960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY71536.1"
                     /translation="MKQPLPGQVSDVDLRLLRVFRTVVDSGGFAAAELQLNIGRSTIS
                     RHIKDLEARLGVSLCRRGRGGFALTPEGSRIYEATLRVLGSIADLRSEIHDLHQRLSG
                     SLQLVLFDKTVTNPQAHIATAIGALRRQAPDVSIEIRIHPLNEIERGILDGRFHVGVL
                     PVYRRSGACEYHPLFDETMYLYCGQGHSLFERADAGTSDAEVQAAAFAGLGFHSDNME
                     RGHEFGLDRRAIAYDQEGVATLILSGAYIGFLPDHYAASFVRQGSMRAIRPRRFTYRC
                     SFGAIVRHDAQRPRLTQTFLELLLAAHQGTARLS"
     gene            complement(3811..4068)
                     /locus_tag="PLW13_13965"
     CDS             complement(3811..4068)
                     /locus_tag="PLW13_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HQY71537.1"
                     /translation="MTDDASRTEIEAAVLRRLLAHLDARKDVQNIDLMNLAGFCRNCL
                     AKWYAAAAAEQGIAIDYDQARERVYGMPYAEWKARYQTEAK"
     gene            complement(4441..5175)
                     /locus_tag="PLW13_13970"
     CDS             complement(4441..5175)
                     /locus_tag="PLW13_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71538.1"
                     /translation="MELALLAAQEGGKLAICARTASKLDAAERDVAALGLGTEVLKVP
                     TDIADREQCRNLVERTVERFGRVDALINSAYVGGGFGPIETADLDDWRATMDINFFGT
                     MKLTQEVIPHMKRQGGGSIVMINTMVTRVPMPTQGGYGASKGALRVATSHLAKELGRY
                     GIRVNSTFMGWMWGEPVENALKFMAKEQGTTVEAVRKQIAANIPLGDIPDDADCAKVA
                     IFLASDYSRAMSGACVDVNGGEYLPV"
BASE COUNT          855 a         1727 c         1614 g          880 t
ORIGIN      
        1 gcgcgcggcc tcgccgtcgc gcgtgaagat gcaggtgccg ttgccgtact cgtgcgcgtc
       61 gatcagcgcc atcgcctctt cctgcgattt cacgcgcacc acgcacagca ccggaccaaa
      121 gatctcttcg ttgtacacgc gcatcgaggg cagcaccctg tcgaagaggc agccgccgag
      181 gaagaagccc tcctcgtgcc ccggcaccgt gaggccgcgt ccgtccacca cgagttgcgc
      241 gccctcttcc acgccctggt ccacgtagcc gcgcactttc tcgtagtgag ccctggtgac
      301 cagcgggccc atgtccatgc caggcaacgt acccggtccg accttgaggt gcgcgaggcg
      361 ctgcttcagt tttgccacca gcgcgtcgcc cacttcgtcg cccaccgcga ccgccaccga
      421 gatcgccatg cagcgctcgc cgcaggagcc gtaggccgcg cccatcagcg cgctcaccgc
      481 gttgtcgatg tcggcatcgg gcatcacgat ggcgtggttc ttggcgccac ccagcgcctg
      541 gatgcgcttg ccgtgcttcg tgccggtctg gtagacgtat tcggcgatcg gcgtggagcc
      601 gacgaagctc acggcctgca cgcgcgggtc ggtgagcagg gtatcgaccg cctccttgtc
      661 gccgttcacg acgttgaaca cgccgtccgg caagccggct tccttgaaga tccgcgcgat
      721 cagcagcgcc gcgctcggat cgcgctccga gggcttcagc acgaaggtgt tgccgcacac
      781 gatcgccatc gggaacatcc acatcggcac catcgccggg aagttgaacg gcgtgatgcc
      841 cgccaccacg cccagcggat ggaactcgga ccaggaatcg atcgcggggc ccacgttctt
      901 cgcgtgctcg cccttcagca gctgcggcgc tgcgcaggcg tactccacca cctccacgcc
      961 gcgctggaac tctccgagcg catcgtcgaa gaccttgccg tgctcctcgg tgatcagttc
     1021 ggcgatcgca ttggcgttct gctccagcag atccttgaaa cggaacatga tccgcgcgcg
     1081 cttgagcggc ggcgtgtcgc gccacgcggg aaacgcggcg gccgcggcgg cgatggcctg
     1141 ttccacggtg gcgcgcgcgg caagcgatac ctcgcgcacg acctgtccgg ttgccggatt
     1201 gaacaccggc tgcgtgcgcg cgccgtcggc aacggccgcg ccgttgatga aatggccgag
     1261 ggtttcgagt ttcagatgca cgttcatgtc agttcaccgt gggtatgaca tcgttgaggg
     1321 tgttgaaaag ctggtcgagg tctgccttct ccgtgacgaa gggcggtgcg aactgcagcg
     1381 tgtcaccgcc ccagcgcacg tagaacccgc gctcccacat cgcgagcgcg atctcgtagg
     1441 gccggcgcag cggttcgccg ggccgggcct cgatctgcag cgcgccggcc aggccgaaat
     1501 tgcggatatc ggtgatgtgc ttcacgccct tcagaccatg cagcccgtcc tcgaaatacg
     1561 gcgccagcgc gcgcaccttg ccgaccatgt cctcgcgctg gaacacgtcc agcgccgcga
     1621 tgcccgcggc acaggcgacc gggtgcgcgg agtaggtgta gccatgggga aactcgacgg
     1681 catattccgc cccaccggcg gccataaagg tccggtagat ctcgccgctt gcgatcaccg
     1741 cccccatcgg caccgcgccg ttggtgaggc acttggccac gttgaggatg tcgggcgtca
     1801 cgccgaaggc ctgcgcgccg aacacgtccc ccgcacggcc gaagccggtg atcacctcgt
     1861 cgaagatcag caggatatcg tgcttcgtgc agagctcgcg caggcgctgc aggtagccct
     1921 tgggcggcac gatcacaccg gccgagccgg agaagggctc gacgatcacg gccgcgatat
     1981 tcgaggcgtc gtgcagagcc accagttcct cgagctggtc cgcgagctcc acgccgttct
     2041 ccggcatccc gcgcgagaac gcgtttgcgg caagcagcgt gtgcggcagg tgatcggcat
     2101 ccagcgtgtg gccgaacagc ttgcggttgc cgccgatgcc gcccaggctg atgccgccga
     2161 agttcacgcc gtggtaaccc ttggcgcgac cgatgaactt ggtcttggtc ggctggccct
     2221 tctggcgcca gtaggcacgc gccatcttca gcgcggtatc ggcggcctcg gatcccgagt
     2281 tggtgaagaa cacgtagtcg agccctggcg gcgccatcgc ctcgagcatg ttggcgaggc
     2341 gaaacgacag cgggtgcccg aactggaacg ccggggcgta gtcgagctcc tggacctgtt
     2401 gcgccaccgc gcgcgagatc tccgtgcgtc catgtccgag gccgcagcac cacaaccccg
     2461 agaggctgtc gaacacgcgc cgcccgtcgt cggtgacgta gtggcagccc tcggcgcgca
     2521 cgatcatgcg cgggttgcgc ttgaactcgc ggttcggcgt gaacggcatc cagtgggctt
     2581 cgagctcctc gcggctgagc gcgggaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnntc cgctcagggc aatggcactc tacgaattgc tcaatgtttc gtaaatgact
     2761 atttttacaa ccatagttgc cagattatga aactttatga aacagccact gcccggacag
     2821 gtatcggacg tcgacctgcg cctgctgcgc gtgttccgca ccgtggtcga cagcggcggc
     2881 ttcgcggccg cggagctcca gctgaacatc ggccgctcga cgatcagccg ccatatcaag
     2941 gacctggaag cacggctcgg cgtgagcctg tgccggcgcg gacgcggcgg cttcgcgctc
     3001 acgcccgagg gcagccgcat ctacgaggcc acgttgcgcg tgctgggctc gatcgcggac
     3061 ctgcgcagcg aaatccatga cctgcaccag cgcctgagcg gcagcctgca gctggtgctg
     3121 ttcgacaaga ccgtcaccaa cccgcaggcg cacatcgcca cggccatcgg cgcgctgcgt
     3181 cggcaggcac ccgacgtgtc gatcgagatc cgcatccacc cgctgaacga gatcgagcgc
     3241 ggcatcctcg atggccgctt tcacgtcggc gtgctcccgg tctaccgccg ctccggagcc
     3301 tgcgagtacc acccgctgtt tgacgaaacg atgtacctct actgcgggca ggggcactcg
     3361 ctgttcgagc gcgcggacgc cgggacaagc gatgccgagg tgcaggccgc cgccttcgcg
     3421 gggctcggct tccactccga caacatggag cgcggccacg agttcggcct ggaccggcgc
     3481 gccatcgcct acgaccagga aggcgtggcc acgttgatcc tctcgggtgc ctatatcggc
     3541 ttcctgccgg accattacgc cgcgagcttc gtgcggcagg gatcgatgcg cgcgatccgc
     3601 ccccggcgct tcacgtaccg ttgcagcttc ggtgcgatcg tgcgccacga cgcacagcgc
     3661 ccgcgcctga cgcaaacctt cctggaattg ttgctggcgg cgcaccaggg cacggcgcgc
     3721 ctatcgtagg gccagaccct gtgggtccgg attcatccgg aggccggcac cgagccggcg
     3781 aaggcagaat ccgtccgcat tcatgcggac ctacttggcc tcggtctggt agcgggcttt
     3841 ccattcggcg taaggcatgc cgtagacgcg ttcgcgtgcc tggtcgtagt cgatcgcgat
     3901 gccctgctcc gcagcggccg ccgcgtacca cttggcaagg caattgcggc agaagccggc
     3961 gaggttcatc aggtcgatgt tctgcacgtc cttgcgcgcg tcgagatggg ctagcaggcg
     4021 gcgcaacacc gccgcctcga tttcggtcct gcttgcatcg tcagtcatgc cagtttgctc
     4081 ccggttcggc cgtaggtccg cattcatgcg ggtatcgcat ggatccgcat gaatgcgaac
     4141 attgtccggc cgaagccgga cccacgaaag aggtctacga tggcagcctc ccgcccgggt
     4201 cacaagcaaa aaaaagcccc gctgagaagc ggggccaagc tttaggagac aacactaacc
     4261 aaacgacaaa tggtgatgac agcaagggga taaaaccatc aatcgccatt cgcagtactg
     4321 agacttgggc atggcggtga agttccacgg cacctgaaaa aaattttccg gcttcgtgaa
     4381 cctccatgat ttttccttga aaatcatgaa gctgccctca tgaaaatttc ccggcccgcg
     4441 tcaaaccggc aggtactcac ccccgttcac atcgacgcag gcgccgctca tcgcgcggct
     4501 gtaatccgag gccaggaaga tcgcgacctt ggcgcagtcg gcgtcgtccg ggatgtcgcc
     4561 cagcggaatg ttggcggcga tctgcttgcg cacggcctcg accgtggtgc cctgctcctt
     4621 cgccatgaat ttcagcgcgt tctcgaccgg ctcgccccac atccagccca tgaacgtcga
     4681 gttcacgcgg atgccgtaac ggcccagttc cttcgcgagg tgcgaggtcg cgacccgcag
     4741 cgcgcccttg gaggcgccgt agccgccctg cgtcggcatg ggaacgcgcg tgaccatcgt
     4801 gttgatcatc acgatcgagc cgccgccctg gcgcttcatg tgcgggatga cttcctgcgt
     4861 cagcttcatg gtgccgaaga agttgatgtc catggtggcg cgccagtcgt ccaggtcggc
     4921 cgtctcgatg ggcccgaagc cgccgccgac ataggcgctg ttgatcagcg catcgacgcg
     4981 gccgaagcgc tcgaccgtgc gctccaccag gttgcggcac tgctcgcggt cggcgatgtc
     5041 ggtgggcacc ttcagcacct cggtgcccag gccaagcgcg gccacatcac gctccgccgc
     5101 atccagcttg gaggccgtac gcgcgcagat cgcgagcttg cctccttcct gagccgcgag
     5161 cagcgccagt tcgata
//
LOCUS       DAORUQ010000207         5136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19847_length_5136_cov_5.537493, whole genome shotgun sequence.
ACCESSION   DAORUQ010000207 DAORUQ010000000
VERSION     DAORUQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19847_length_5136_cov_5.537493"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..898)
                     /locus_tag="PLW13_13975"
     CDS             complement(98..898)
                     /locus_tag="PLW13_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQY71539.1"
                     /translation="MRAPGIPVVGIPCDIFDKGLHPFHGVGEKYINAVAHGARALPLL
                     IPGLGRGRDLEPIGSLLDMAGLLDSLDGIFLTGSPSNVEPARYGGEPSAPGTSHDPQR
                     DATTLPLIRLALERGIPLFCVCRGMQELNVALGGTLHQRVQEVPGMMDHREDKQASRE
                     LQYAPAHEVIIEEGGVLAGLGVGSRHRVNSLHAQGVDRLAPGLRVEARAPDGLIEALS
                     VEGSRALALGVQWHPEWLFREHELSRALFGAFGAAVWSQYRGKDAKSA"
     gene            1049..2419
                     /locus_tag="PLW13_13980"
     CDS             1049..2419
                     /locus_tag="PLW13_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQY71540.1"
                     /translation="MTRANRSKQHWQALDRAHHLHPFTDFHELGEQGTRVITRAEHIY
                     IHDIDGREYLDAMSGLWCCNLGYSRREIIEAVTRQLQELPFYNNFFQCAHPPSIELAQ
                     VLAEVAPAHMKHVFFTNSGSEGNDTVVRMVRRYWDLEEQPQKRWIISRVNAYHGSTIA
                     GASLGGMSFVHKQFEPLPYVTHIRQPYWYGEGAGLSPEEFGVQAARALEARIEELGPD
                     NVAAFIAEPVQGAGGVIIAPESYWPEVRRVLAKYDILLVMDEVICGFGRTGKWFGNVH
                     YGMKPDLMTFAKAVTNGYQPLGGVMVGERVAEVLTRRGGEFGHGFTYSGHPAACAAGL
                     ATLKILREENVIGNVEAVSAPHLARRWAELADHPLVGEARTLGLFGALELVKNKSTRE
                     RSAPGGRAGAVCRQASLNNGLVMRATGDTMIIAPPLICTTAELDMLVERAKAALDETA
                     RELGAG"
     gene            2497..2745
                     /locus_tag="PLW13_13985"
     CDS             2497..2745
                     /locus_tag="PLW13_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQY71541.1"
                     /translation="MGILSWIVMGLIVGVLAKFIMPGKDPGGFVITVALGIGGAFVGG
                     YIASFLGIGAVSGINVASIFIATLGAIVLLAVYRALKK"
     gene            2920..3330
                     /gene="rnk"
                     /locus_tag="PLW13_13990"
     CDS             2920..3330
                     /gene="rnk"
                     /locus_tag="PLW13_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQY71542.1"
                     /translation="MSSQPAITVSSLDLQRIEHVLRAESASHLPGIDALQAELDRANV
                     VEPVDMPPGIVTMNSTVRFCDDATGNRHELTLVYPDGAGAPGTVSILAPTGSALLGLS
                     VGQSIEWQVPGGRKLQLRVLEVVRQPEAEGEYHR"
     gene            3358..4233
                     /locus_tag="PLW13_13995"
     CDS             3358..4233
                     /locus_tag="PLW13_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY71543.1"
                     /translation="MNRTILSATDFSADARQAAERAALLCAGGAMAGSTLLHVMQASW
                     LDSLRRLVKLPPEAEAAMLAEATAELGHWASEIGARTGFVPHTGVRIGSVVDTLLREA
                     EEHELLALGARGRHPLRDFALGTTAEHLLRRTRKPLLVVRRPAASGYRRVLVALDFSP
                     HSRTALEWAVRLGPLAEVHALHVFEMPFEGMMHYSGVDSALVEQYRREAHAEATGEMR
                     RFIADTAIDPRQLRTEIAGGEHIPSALQERALALDADLVVVGKHGKSFAEQLLLGSVT
                     LHLLAQCPCDVLVAQ"
     gene            4246..>5136
                     /locus_tag="PLW13_14000"
     CDS             4246..>5136
                     /locus_tag="PLW13_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY71544.1"
                     /translation="MPHEPALITTIAAAFGLALILGFLAVRLRLPALVGYMLAGVILG
                     PATPGFVADIELSQQLAEIGVMLLMFGVGLHFSLADLLAVRRIALPGAIAQIAAATVL
                     GAGLAHLWGWSPGAGLVFGLALSVASTVVLLRALESRGVLDSINGRIAVGWLVVEDLV
                     MVLVLVMLPPLAGWLGGTQGDTAQDGLLVPFLVTIGKVAAFVGLMLVVGRRVFPWLLW
                     QVARTGSQELFTLCVIAAAISIAWGAAAIFGVSFALGAFFAGTVLRESALSHRAAGET
                     LPLRDAFSVLFFVSVGMLFDP"
BASE COUNT          757 a         1813 c         1755 g          811 t
ORIGIN      
        1 ccggattctg ttgcaggaag gtgtcgagtg catgcatggc ggcaaccgtg atcaaggtgc
       61 gaatcgtgaa cgctcaggag ccggccgccc ggccgcctca ggcactttta gcatccttgc
      121 cgcgatattg cgaccacacc gccgcaccga aggccccgaa cagggcccgc gacagctcgt
      181 gctcgcgaaa cagccactcc gggtgccact gcaccccgag cgcgagcgcg cgggagccct
      241 ccacgctgag cgcctcgatc agcccgtcgg gcgcgcgcgc ctcgacgcgc agccccggcg
      301 cgagccggtc gacgccttgg gcgtgcagcg aattgacgcg atgacgcgac ccgacgccca
      361 gtcccgccag cacgccgccc tcctcgatga tcacctcgtg cgccggcgcg tactgcagct
      421 cgcggctcgc ttgcttgtcc tcgcgatggt ccatcatgcc gggcacttcc tgcacccgct
      481 ggtggagcgt gccgccgagg gcgacgttca gttcctgcat gccgcggcag acgcagaaca
      541 gcgggatccc gcgttcgagc gcgagccgga tcagcggcag cgtggtcgcg tcgcgttgcg
      601 ggtcgtggga ggttcccggc gcgctcggct caccgccgta gcgcgcgggc tccacgttcg
      661 acgggctgcc ggtgagaaaa attccgtcga ggctgtcgag cagcccggcc atatcgagca
      721 gcgacccgat cggttcgagg tcgcgcccgc ggccaagacc cggaatcagc aacggcaggg
      781 cgcgcgcgcc atgcgccacc gcgttgatgt acttttcacc cacgccgtga aagggatgca
      841 ggcccttgtc gaagatgtca cagggaatgc cgaccaccgg gatgccgggt gcgcgcatgc
      901 gtatcctctt tccgctgcgc taccctttgg gggtagcgtc gttatcgctc gaatttggca
      961 tattgcggca ttctaagccc tgcgtggtcg ccatccccat accaccagcg gggtaagtcc
     1021 gcgcggcgga ccgacacgag gacacacgat gacgcgcgcc aaccgatcca agcagcactg
     1081 gcaggcgctc gaccgcgccc atcacctgca ccccttcacg gacttccacg agctgggcga
     1141 gcagggcacg cgcgtgatca cgcgtgccga gcacatctac atccatgaca tcgacgggcg
     1201 cgagtatctc gacgccatgt cgggactgtg gtgctgcaac ctcggctaca gccgccgcga
     1261 gatcatcgag gcggtcacgc gccagctgca ggagctgccc ttctacaaca acttcttcca
     1321 gtgcgcgcac ccgccctcga tcgagctggc gcaggtgctg gccgaggtcg cgccggcgca
     1381 catgaagcac gtgttcttca cgaactcggg cagcgagggc aacgacaccg tggtgcgcat
     1441 ggtgcgccgc tactgggacc tggaggaaca gccgcaaaag cgctggatca tcagccgcgt
     1501 caacgcctac cacggcagca cgatcgccgg cgcgagcctc ggcggcatga gcttcgtgca
     1561 caagcagttc gagccgctgc cctacgtgac gcacatccgg cagccgtact ggtacggcga
     1621 gggcgcgggc ctgtcgccgg aggaattcgg cgtgcaggcc gcgcgcgcgc tcgaggccag
     1681 gatcgaggaa ctcggccccg acaacgtggc cgcgttcatc gccgagccgg tgcagggcgc
     1741 cggcggcgtg atcatcgcgc ccgagagcta ctggcccgaa gtgcgccgcg tgctcgcgaa
     1801 gtacgacatc ctgctggtca tggacgaagt gatctgcggc ttcgggcgca ccggcaagtg
     1861 gttcggcaat gtccactacg gcatgaagcc cgacctgatg accttcgcca aggccgtcac
     1921 caacggctac cagccgctcg gcggcgtgat ggtcggcgag cgcgtcgccg aggtactgac
     1981 caggcgcggc ggagaattcg gtcacggctt cacctattcc ggccatcctg ccgcctgcgc
     2041 cgcagggctc gcgacgctga agatcctgcg cgaggaaaac gtcatcggga acgtcgaggc
     2101 cgtctccgcc ccgcatcttg cccggcgctg ggcggagctc gccgatcatc cgctggtcgg
     2161 cgaggcgcgc acacttgggc tcttcggcgc gctcgaactg gtcaagaaca agagcacgcg
     2221 cgagcgttcc gcgcccggcg gtcgcgccgg cgccgtatgc cgccaggcca gcctgaacaa
     2281 cgggctggtg atgcgcgcca ccggtgacac catgatcatt gcgccgccgc tgatctgcac
     2341 caccgccgag ctcgacatgc tggtcgaacg cgcgaaggcg gcgctcgacg agacggcgcg
     2401 cgaactcggg gccggctgaa gcgcaggcgc cttgcgcgcg ccacgcaaag gcaatagggt
     2461 aaaggccggc caacaacccg aacgaggata tcgatcatgg gcattctttc gtggatcgtg
     2521 atggggctga tcgtgggcgt gctcgccaag ttcatcatgc ccggcaagga tccgggcggc
     2581 ttcgtcatca cggtggcgct cggcatcggc ggcgccttcg tcggtggcta catcgcctcg
     2641 ttcctgggta tcggcgccgt cagcggtatc aatgtcgcca gcatattcat cgccacgctg
     2701 ggcgcgatcg tgctgctcgc ggtatatcgc gcgctgaaga agtgacgccg gggcccgcgc
     2761 gccccgcatc gcctccctgc ccgttcgctt cccgcggcgg ggtgtctttg tcgtgacgaa
     2821 gcggttacca tctcgccatc ccgctcatca cccacgcgcc ggcacccgcc ggcgcgtggc
     2881 gttttgcgcc ggcacaacga tcacgcaagg agattgacca tgagctcgca gcccgccatc
     2941 accgtatcca gcctcgatct gcagcgcatc gagcacgtgc tgcgcgccga gtcggcgtca
     3001 cacctgcccg gcatcgacgc actgcaggcc gaactggatc gcgccaacgt ggtcgaaccc
     3061 gtggacatgc cgcccgggat cgtcacgatg aactccaccg tgcgtttctg cgacgacgcc
     3121 accgggaacc ggcacgagct cacgctggtc taccccgatg gcgccggcgc gcccggcacg
     3181 gtctcgatac tggcgcccac gggcagcgcc ctgctcgggc tctccgtggg gcagtcgatc
     3241 gaatggcagg tccccggcgg gcgcaagctg cagctgcgcg tgctcgaggt ggtgcgccag
     3301 cccgaggccg agggcgaata ccatcgctga cgccggccac gtcacggaga tcgcacgatg
     3361 aaccggacca tcctcagcgc cacggatttc tccgccgacg cgcgccaggc ggcggagcgc
     3421 gcggcactgc tgtgcgccgg cggcgcgatg gccggcagca cgctgctgca cgtgatgcag
     3481 gcctcgtggc tcgactcgct gcgccgcctg gtgaagctgc cgcccgaggc ggaagcggcg
     3541 atgctcgccg aagccacggc agagctgggg cactgggcct cggagatcgg tgcccgcacc
     3601 ggcttcgtgc cgcacaccgg cgtgcgcatc ggcagcgtcg tcgatacgct gctgcgcgag
     3661 gccgaggagc acgaactgct cgcgctgggc gcccgcggcc ggcatccgct gcgcgatttc
     3721 gccctaggca ccacggccga gcacctgttg cgccgcacgc gcaagcccct gctggtggtg
     3781 cgccgtccgg ccgcgtccgg ctaccggcgg gtgctggtcg cgctggattt ttcgccgcat
     3841 tcgcgcaccg cgctcgagtg ggccgtgcgg ctcggcccac tcgccgaggt gcacgcactg
     3901 cacgtcttcg aaatgccctt cgaggggatg atgcactact ccggcgtcga ttcggcgctc
     3961 gtcgagcagt accgccgcga ggcccacgcg gaggcaaccg gcgaaatgcg ccgcttcatc
     4021 gccgacaccg ccatcgaccc acggcagctg cgcaccgaga tcgccggcgg cgagcacatt
     4081 cccagcgcgc tgcaggaacg ggcgctcgcc ctcgatgccg acctggtcgt ggtcggcaag
     4141 cacggcaagt cgttcgccga gcagttgctg ctcggcagcg tgacgctgca cctgctcgcc
     4201 cagtgcccct gtgacgtgct cgtcgcgcag tagcggaacc ggctagtgcc ccacgaaccg
     4261 gcgctgatca ccaccatcgc cgccgccttc gggctggcgc tgatcctggg cttcctggcg
     4321 gtgcgcctgc ggctgccggc actggtgggc tacatgctcg ccggcgtgat cctcggcccg
     4381 gccacaccgg gtttcgtcgc cgacatcgag ctctcgcagc agctggccga gatcggcgtg
     4441 atgctgctga tgttcggggt gggattgcac ttctcgctgg ccgacctgct cgcggtgcgc
     4501 cgcatcgcgc tgccgggcgc catcgcgcag atagccgccg ccaccgtgct gggcgcgggc
     4561 ctggcgcacc tctggggctg gagccccggc gcggggctgg tgttcgggct cgcgctctcg
     4621 gtcgcgagca ccgtggtgct gctgcgcgcg ctggagagcc gcggcgtgct cgactccatc
     4681 aacgggcgca tcgccgtggg ctggctggtc gtcgaggacc tggtgatggt gctggtgctg
     4741 gtgatgctgc cgccgctcgc gggttggctc ggcggcaccc agggcgacac cgcgcaggac
     4801 gggctgctgg tcccgttcct ggtcacgatc ggcaaggtcg cggccttcgt ggggctgatg
     4861 ctcgtggtcg gcaggcgcgt gttcccgtgg ctgctgtggc aggtcgcgcg caccggctcc
     4921 caggagctgt tcacgctgtg cgtgatcgcg gccgcgatca gcatcgcctg gggagccgcc
     4981 gcgatcttcg gcgtgtcctt cgcgctcggc gccttcttcg ccggcacggt gctgcgcgag
     5041 tcggcgctga gccaccgcgc cgccggcgag acgctgccgc tgcgcgacgc gttctcggtg
     5101 ctgttcttcg tctcggtggg catgctcttc gacccg
//
LOCUS       DAORUQ010000208         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_19873_length_5131_cov_4.874310, whole genome shotgun sequence.
ACCESSION   DAORUQ010000208 DAORUQ010000000
VERSION     DAORUQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19873_length_5131_cov_4.874310"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLW13_14005"
     CDS             complement(<1..611)
                     /locus_tag="PLW13_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71545.1"
                     /translation="MPAGWRAAMTGSAQDVFVRAQREWFTKLVDAGYATPHWPQGWPG
                     GGRSLAEQKVIYEEIARADAPRLILYFVSLYHAACTLMECGTPAQRDRYLPGILAGEI
                     WCQGFSEPNAGSDLASLKTRAELRGDRYVINGQKIWSTMAQYADRCLLLVRTGKSGPP
                     QAGLTYLLLDMRTKGVSVRPISQITGDDEFAEMCANLANHQLAA"
     gene            complement(704..1795)
                     /locus_tag="PLW13_14010"
     CDS             complement(704..1795)
                     /locus_tag="PLW13_14010"
                     /inference="COORDINATES: protein motif:HMM:NF014954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY71546.1"
                     /translation="MSFPGLRLLAARIPPRWEDATPAWMSDAIASRCPGARVAALELV
                     TRDDGTNRRACFALRYAAGAGPATVFLKAHSPHHRWVHLRNGNLFNEARLFACGVALP
                     VDHPLVYKAVVDRLRLDFLLVMEDLAQRGADPRDATRPLSVDQVAHGLRALARLHSAF
                     WGFSGRTHPQLRWVKTWKPTKGWQVGLRKRVPLGLQRGAGQLPAQVTRHDGEQIVDLW
                     ARYVGSLSRGPMTLLHGDAHIGNTYVLPDGEVGFLDWQVVRRGGWSQDVGYFLVGALT
                     EEDRRGHEAALLEEYRGALQVPASERPSAAQAWLQYRATPAYGMPVWLSTLGSDGYQS
                     REVSLALARRYSSAFAELETPSALASIGA"
     gene            complement(1792..2103)
                     /locus_tag="PLW13_14015"
     CDS             complement(1792..2103)
                     /locus_tag="PLW13_14015"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HQY71547.1"
                     /translation="MSGEFTRFCTVAELPPESKKAAKLNNTWVLVCHTDGRLFAVSGV
                     CSHQVKPLVNGRVRHCMITCPVHGARFNLETGAALNLPATQPITTYELRVVDDWIEVR
                     V"
     gene            complement(2110..>3300)
                     /locus_tag="PLW13_14020"
     CDS             complement(2110..>3300)
                     /locus_tag="PLW13_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY71548.1"
                     /translation="TWQFACREEHIPEVGDYHVYDIGRHSFIVTRVAENEVRAYYNAC
                     LHRGTKLRASGTEGNTTEFKCSFHGWSWNLDGSQKELLCPWDFPHVDIATNKLPQVRV
                     ELLGGFVFINMDPDAPTLADYIGPAAMAHIREWKLEDRYIYCHVAKRLPANWKLSIAA
                     FMEAYHVLDTHPQVSVTNADANSQYDVYGEHVNRFISPLGVLSPHHYGKYTEQDIMDQ
                     FTLGDSSALGDVERKLPEGGTARQAMADMFRGMFEKATNTDLGGVSDAELLDCFSYTL
                     FPNIFLFPGISLPMVYRFRPDPRDHRWTLYEVLFLRPVPLDGKRPYPAEPVRLTDEQS
                     FTEAEGMDPGFGAILDQDTDNLILQQEGIEASAKRGLTLGNYQEIRIRHFERAVDRYV
                     GTKG"
     assembly_gap    3301..3400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3401..3677)
                     /locus_tag="PLW13_14025"
     CDS             complement(<3401..3677)
                     /locus_tag="PLW13_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71549.1"
                     /translation="MNKPIGTEARNLARGAARYPGAPSTQEIIASDRVKAPGWVCSES
                     YEFLGDDDISTDRYTEAAYAEREFERLWTRTWQFACREEHIPEVGDYH"
     gene            3880..4356
                     /locus_tag="PLW13_14030"
     CDS             3880..4356
                     /locus_tag="PLW13_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY71550.1"
                     /translation="MIRGIHHVGVHVRDLDRMIRFYREAFGFELVGEPFGWRDNDFID
                     RIIDVPNSAARGAMLRAGTCFMELFEYSEPAPGVTAPLNAYDKGYTHFCVDVTDIEHE
                     FERLKGLGMTFGQPAPIDAGHVKTIYGRDPEGNLIEIQQTAENCDFPLHSLPPVPR"
     gene            4376..>5131
                     /locus_tag="PLW13_14035"
     CDS             4376..>5131
                     /locus_tag="PLW13_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQY71551.1"
                     /translation="MTRSVTTLDDKYLQSGGRVFLSSNQALVRLPLDQARRDRAAGLK
                     TAGYISGYRGSPLGVYDSALWGAQKLLDQHQVVFRPGLNEELAVSAIRGTQQLPWFGK
                     PEFQGIFGLWYGKGIGVDRACESLKLGNFEGAAAHGGFLAVAGDDHGGKSSDSAHQSE
                     HTLIAAMLPILYPATVGEIIKFGLFGWAMSRYSGCYVGLKCITDTLDLTACVDLPDPH
                     RPYLTPTDIQLPPEGLNLRPNLPPLVEEDWLVNH"
BASE COUNT          804 a         1718 c         1694 g          815 t
ORIGIN      
        1 gcggcaagtt ggtgattggc gagatttgcg cacatctcgg cgaattcgtc atcgccggtg
       61 atctgcgaaa ttgggcgcac cgacacaccc ttcgtgcgca tgtcgaggag caggtaggtc
      121 aatcccgcct gcggcgggcc ggatttgccc gttctgacca gcaacaggca ccggtcggca
      181 tactgcgcca tggtcgacca gatcttctgg ccgttgatca cgtagcggtc gccacggagt
      241 tccgcccgcg tcttcagcga tgccaggtca gaaccggcat tgggctccga gaatccctgg
      301 caccagatct cgccggcgag gatgccgggc agatacctgt cgcgttgggc cggcgtaccg
      361 cattccatca aggtgcaggc ggcatggtag agcgacacga agtagaggat caggcgcggc
      421 gcgtcggcgc gcgcgatctc ctcgtagatg actttctgct cggccagcga gcggccgccg
      481 cccggccagc cctgcggcca gtgcggggtg gcatagccgg catcgaccag cttcgtgaac
      541 cactcgcgct gcgcgcgcac gaagacatcc tgcgccgatc cggtcatcgc cgcgcgccag
      601 ccggcgggca cgttccgcgc gagccacgcc ctgacctgcg cgcgcagcgc ctcgatattg
      661 ctgtccgact ccattgtccg cccttgccgt tacgcccgct tgatcaggca cctatgctag
      721 ccagcgccga gggggtttca agctccgcga acgcggacga ataccggcgc gccagcgcga
      781 gcgaaacctc gcgcgactgg taaccgtcgc tgccgagcgt ggagagccag accggcatgc
      841 cgtaagccgg cgtggcgcgg tattgcagcc aggcttgggc ggcggaggga cgctcgctgg
      901 caggaacctg cagcgccccg cggtattcct cgagcaaggc cgcttcgtgg ccgcgacggt
      961 cttcctcggt gagcgcaccg accaggaaat aacccacgtc ctgcgaccag ccgccgcggc
     1021 gcaccacctg ccagtcgagg aaacccacct cgccgtcggg caacacgtag gtgttgccga
     1081 tgtgcgcgtc gccgtgcagc agcgtcatcg gcccccggct caacgaaccc acgtagcgcg
     1141 cccacaggtc cacgatctgc tcgccgtcat ggcgcgtcac ttgcgcgggc agctggcccg
     1201 cgccgcgctg caggcccagc ggaacgcgct tcctgagccc gacctgccag cccttcgtgg
     1261 gcttccaggt cttcacccag cgcagttgcg ggtgcgtcct gcccgagaag ccccagaacg
     1321 cgctgtgcag ccgcgccagg gcgcgcaagc cgtgcgccac ctggtccacg ctcagcgggc
     1381 gcgtggcatc gcgcggatcg gcgccgcgct gcgcgagatc ctccatcacg agcaggaagt
     1441 ccagccgcag ccggtccacg acggccttgt agaccagcgg gtggtccacc ggcagcgcaa
     1501 cgccgcaggc gaacaggcgc gcctcgttga agaggttgcc gttgcgcagg tgcacccagc
     1561 ggtggtgcgg cgaatgcgcc ttgaggaata ccgtcgcggg cccggcgccg gcggcgtagc
     1621 gcagggcgaa gcacgcgcgg cgattggtgc cgtcgtcgcg ggtcacgagc tcgagcgccg
     1681 cgacgcgggc gccggggcag cggcttgcga tcgcatcgct catccatgcc ggcgtcgcgt
     1741 cctcccagcg cggcgggatc ctcgccgcca gcaggcgaag acccgggaag ctcatacgcg
     1801 cacttcgatc cagtcgtcca cgacgcgcag ctcgtaggtc gtgatgggct gggtcgccgg
     1861 caggttgagc gcggcaccgg tctcgaggtt gaagcgcgcg ccgtgcaccg ggcaggtgat
     1921 catgcagtgg cgcacccgcc cgttcaccag cggcttgacc tggtgcgagc agacgcccga
     1981 gaccgcgaac agccggccat ccgtatggca caccagcacc caggtgttgt tcagcttcgc
     2041 ggctttcttc gactccggcg gcagctccgc caccgtgcag aatcgcgtga attcacccga
     2101 caagatcgat taccccttgg tcccgacgta cctgtcgacg gcgcgctcga agtggcggat
     2161 gcgtatctcc tggtagttgc cgagcgtgag tccgcgcttg gcgctggcct cgatgccttc
     2221 ctgctgcagg atcaggttgt ccgtgtcctg gtcgaggatg gcgccgaagc ccggatccat
     2281 gccctcggcc tcggtgaagg actgctcgtc ggtgaggcgc accggctcgg ccggatacgg
     2341 gcgcttgccg tcgagcggca ccgggcgcag gaacagcacc tcgtacagcg tccagcggtg
     2401 atcgcgcggg tcggggcgga agcggtagac catcggcagc gagatgccgg ggaacaggaa
     2461 gatgttcggg aacagcgtgt acgagaagca gtcgagcaac tccgcatccg acacgccgcc
     2521 gagatcggta ttggtggcct tctcgaacat gccgcggaac atgtcggcca tggcctggcg
     2581 cgccgtgccg ccctcgggca gtttgcgctc gacatcgccg agcgcgctgc tgtcgcccag
     2641 cgtgaactgg tccatgatgt cctgctcggt gtacttgccg tagtgatgcg ggctcagcac
     2701 gcccagcggc gagatgaagc ggttcacgtg ctcgccgtag acgtcgtact gcgagttggc
     2761 gtcggcgttc gtcaccgaga cctgcgggtg cgtgtcgaga acatggtagg cctccatgaa
     2821 cgcggctatc gacagcttcc agttggccgg caggcgcttg gcaacgtggc agtagatgta
     2881 gcggtcctcg agcttccact ccctgatgtg cgccattgcc gcggggccga tgtagtcggc
     2941 cagcgtgggc gcgtcggggt ccatgttgat gaacacgaaa ccgccgagca gctcgacgcg
     3001 gacctgcggc agcttgttcg tcgcgatgtc gacgtgcggg aagtcccacg ggcacagcag
     3061 ttccttctgc gagccgtcga ggttccagct ccagccgtgg aagctgcact tgaactcggt
     3121 ggtgttgccc tcggtgcccg aggcgcgcag cttggtgccg cgatgcaggc aggcgttgta
     3181 gtaggcgcgg acttcgtttt cggccacgcg cgtgacgatg aacgagtgcc ggccgatgtc
     3241 gtagacgtgg tagtcgccga cttccgggat gtgctcctcg cggcaggcga actgccaggt
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgtggtagtc gccgacttcc
     3421 gggatgtgct cctcgcggca ggcgaactgc caggtacgcg tccacagccg ctcgaattcc
     3481 cgctcggcat aggcggcctc ggtgtagcga tccgtggaga tgtcgtcatc gccgaggaac
     3541 tcgtaggatt cggagcacac ccagcccggc gccttgaccc ggtcgctcgc gatgatttcc
     3601 tgcgtgctcg gcgcgccggg atagcgggcc gcacccctgg cgagattccg cgcttcggta
     3661 ccgatgggct tgttcattgg gatcctccaa atgcctgcag ctcacgctcg cgccgggagg
     3721 ggcgatgccg gccgcgatta tgccacccgg gcacgacgcg ccaaaatgac cacgcaggca
     3781 tacacacttg cctgatcctg ccagcccctt caatggcctc gcggcgcgcc gctacactac
     3841 cgccacgcaa cgctcggcaa ccgcccggag gacgaatcga tgatcagggg catacaccac
     3901 gtgggggtcc acgtgcgcga cctcgaccgg atgatccggt tctaccgcga agccttcggc
     3961 ttcgagctgg tcggcgagcc gttcggctgg cgcgacaacg acttcatcga ccgcatcatc
     4021 gacgtgccga actccgcggc gcgcggcgcc atgctgcgcg ccggcacctg cttcatggaa
     4081 ctgttcgagt acagcgagcc ggcgcccggc gtgaccgcgc cgctgaacgc ctacgacaag
     4141 ggctacacgc acttctgcgt cgacgtcacg gacatcgagc acgagttcga gcgcctgaaa
     4201 gggctcggca tgaccttcgg ccagccggcg ccgatagatg ccggccacgt gaagacgatc
     4261 tacggccgcg atcccgaggg caacctgatc gagatccagc agacggcgga gaactgcgac
     4321 ttcccgctgc acagcctgcc gccggttccc cgctagcggc gcgcgggcgg cgccgatgac
     4381 acgctccgtg accacgctcg acgacaagta cctgcagtcg ggcggacgcg tcttcctctc
     4441 gtccaatcag gccctggtgc gcctgccgct cgaccaggcg cggcgcgacc gcgccgccgg
     4501 gctgaagacc gccggttaca tctcgggcta tcgcggctcg ccgctggggg tctacgactc
     4561 cgcgctgtgg ggcgcgcaga aactgctgga ccagcaccag gtggtgttcc ggccgggcct
     4621 gaacgaggag ctcgcggtca gcgcgattcg cggcacccag cagctgccgt ggttcggcaa
     4681 gccggagttc cagggcatct tcggcctgtg gtacggcaag ggcatcggcg tcgatcgcgc
     4741 ctgcgaatcg ctgaagctcg gcaatttcga gggcgccgcc gcgcacggcg gcttcctggc
     4801 cgtggccggc gacgatcacg gcggcaagtc ttccgacagc gcgcaccagt ccgagcacac
     4861 gctgatcgcg gccatgctgc cgatcctcta ccccgccacc gtgggcgaga tcatcaagtt
     4921 cggcctgttc ggctgggcca tgtcgcgcta cagcggctgc tatgtcgggc tgaaatgcat
     4981 caccgacacg ctggacctga cggcctgtgt ggatctgccc gatccgcatc gcccctacct
     5041 cacgcccacc gacatccagc tgccgcccga ggggctcaac ctgcgcccga acctgccgcc
     5101 gctggtggag gaggactggc tggtcaacca c
//
LOCUS       DAORUQ010000209         5106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20038_length_5106_cov_6.831519, whole genome shotgun sequence.
ACCESSION   DAORUQ010000209 DAORUQ010000000
VERSION     DAORUQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20038_length_5106_cov_6.831519"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLW13_14040"
     CDS             complement(<1..374)
                     /locus_tag="PLW13_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQY71552.1"
                     /translation="MAEAQRMEVVLCWHMHQPEYRDLRSGQYQFPWTYLHAVKDYVDM
                     AAHLEAHPGARAVVNFAPVLLEQIADYSAQLEDCLGRGSAIRDPLLAALAEPPMPASP
                     DARSALMKQCLRANRQRIIERFP"
     gene            complement(367..1632)
                     /gene="glgC"
                     /locus_tag="PLW13_14045"
     CDS             complement(367..1632)
                     /gene="glgC"
                     /locus_tag="PLW13_14045"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQY71553.1"
                     /translation="MTPNRSPRFVSRLTRDTLALILAGGRGSRLKQLTDWRAKPAVPF
                     GGKFRIIDFTLSNCVNSGIRQVAVLTQYKSHSLIRHIQHGWSFLRAELGEFVEVLPAQ
                     QRIETSWYEGTANAVLQNLDIIHRHAPEFVLILAGDHVYKMDYGSMLAAHAESGADVT
                     VGCIEVPREEASAFGVMAVDAQWRITEFAEKPEHPQPLPGNPERALASMGIYIFNSRM
                     LFDELARDRHAEASTHDFGRDIIPSLIRRYNVFAYPFRDPQTGAQAYWRDVGTVDSFW
                     QANMELIGVTPELNLYDHDWPIWTYQEQSPPAKFVFDDEGRRGSAVDSMVSGGCIVSG
                     ASVKRSLLFSNVRVHSYCTVADSVIFPDVDIGRRSRIRRAVIDKRCVIPPGTTIGFDP
                     VEDARRFHVSPRGIVLVTPEMMGQELHHG"
     gene            1762..3960
                     /gene="glgB"
                     /locus_tag="PLW13_14050"
     CDS             1762..3960
                     /gene="glgB"
                     /locus_tag="PLW13_14050"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019409752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQY71554.1"
                     /translation="MHRSPDLDHAAADAALLAREDVRRLLASRLHDPFALLGRHGEEI
                     RLWRPWAVAAWVGAERVPARRVGTSGLFVLRHDGAPGPHPEVHWRDVAGALHCDIDPY
                     GFPPQIGDLDLHLFGEGRHRHAYRMLGAHPCTVDGIAGTRFATWAPNAERVSVVGDFN
                     QWDGRCHPMRVRGASGVWELFLPGVAPGALYRFELRSRHDGSLHLKADPYGQQFERRP
                     HTASIVTAPPAHAWQDSEWMERRREWDWLHAPVSVYELHLGSWQRREDGEFLDYRELA
                     HRVAEHAIWLGFTHIELMPVTEHPLDASWGYQATGYFAPTSRFGSPDDFRYFVDHCHQ
                     RGIGVLLDWVPAHFPRDAHALARFDGTPLYEHADPRRGEHRDWGTLIYDFGRNEVRNF
                     LLASANCWLEEFHIDGLRVDAVASMLYLDYSREPGDWTPNVHGGRENLEAMQFLRELN
                     ELTHGEHPGTLTIAEESTAWPQVTRPVWLGGLGFSMKWNMGWMHDTLGYMAHDPVHRV
                     YHHPELTFGLLYAFTENFLLPFSHDEVVHGKGSMLGKMPGDDWQRFANLRLLYLYQYT
                     YPGAKLMFMGGEFAQGREWNHAQALEWAALEGERERGVFALVRDLNHLYRDQFALHGH
                     GFEQQGFEWIDCHDATQSVISYLRRAGDECCVVVLNFTPVPRRGYRIGVPESGSYREL
                     LNTDSRYYAGSDLGNGAPLTAVAEPLMGRPFSLGITLPPLAGLVLRREGG"
     gene            3965..>5106
                     /gene="glgA"
                     /locus_tag="PLW13_14055"
     CDS             3965..>5106
                     /gene="glgA"
                     /locus_tag="PLW13_14055"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQY71555.1"
                     /translation="MRVLFVTSEAHPLMKTGGLADVSASLPRALQSLGHQVTLLMPAY
                     RDALAAAGPLGVKLAATRDVDGRHVRLLQTRLPGSRNKVWLLDCPALFDRPGNPYHDA
                     SGLAWADNAERFMLLCRVGALLADDAFGLGWRPEVVHCNDWQSALVPLLLHARHPRPA
                     LVFTIHNLAYQGLFPREEFMRLGIAPDYWHPDALEFHGQLSFIKGGIVFADRVTTVSP
                     SYAREIQGAEFGHGLDGLLRHRAPVLSGILNGIDTTAWNPSRDPALALNYGATTLARK
                     RQNKIALQAETGLDTDPATPLFAFIGRLAEQKGIDLLLQALPVLLEHPAQVVMLGSGD
                     AAYETALAALADAHSGRFALRIGYDEVLAHRIEAGADIFLMPSRFEP"
BASE COUNT          783 a         1787 c         1722 g          814 t
ORIGIN      
        1 gggaagcgtt cgatgatgcg ctggcggttg gcgcgcaggc actgcttcat cagcgcgctg
       61 cgcgcatcgg gcgatgccgg catcggcggc tcggcgagcg cggcgagcaa cgggtcgcgg
      121 atggcgctgc cgcggcccag gcagtcctcg agctgcgccg agtagtcggc gatctgctcc
      181 agcagcaccg gggcgaaatt caccaccgcg cgcgccccgg ggtgcgcctc gaggtgggcc
      241 gccatatcga cgtagtcctt caccgcatgc aggtaggtcc acgggaactg gtactgcccg
      301 ctgcgcaggt cacggtactc gggctggtgc atgtgccagc acagcacgac ttccatgcgc
      361 tgcgcctcag ccatggtgga gctcctgtcc catcatctcc ggcgtgacca gcacgatgcc
      421 ccggggcgag acgtggaagc gtcgcgcgtc ctccaccgga tcgaaaccga tagtcgtgcc
      481 cggcggaatc acgcagcgct tgtcgatcac ggcgcggcgg atgcgcgaac gccggccgat
      541 gtcgacgtcg gggaaaatca ccgaatccgc gacagtgcag tacgaatgca cgcgcacgtt
      601 cgagaacagc agcgagcgct tcacgctggc tcccgagacg atgcagccac ccgagaccat
      661 cgagtccacg gccgatccgc gccgcccctc gtcgtcgaac acgaacttcg ccggcggcga
      721 ctgctcctgg tatgtccaga tgggccagtc gtggtcgtag aggttgagct cgggcgtgac
      781 gccgatcagc tccatgttcg cctgccagaa ggaatccacc gtgcccacgt cgcgccagta
      841 ggcctgcgcg ccggtctgcg ggtcgcggaa aggataggcg aacacgttgt agcgccggat
      901 gagcgagggg atgatgtcgc ggccgaaatc gtgcgtcgat gcctcggcat ggcggtcgcg
      961 cgccagctcg tcgaacagca tgcgcgagtt gaagatgtag atgcccatcg aggccagcgc
     1021 gcgctccgga ttgccgggca gcggctgcgg atgctcgggc ttctcggcga actcggtgat
     1081 gcgccactgc gcgtccaccg ccatgacccc gaaggcgctc gcctcctccc gcggcacctc
     1141 gatgcaaccg accgtcacgt cggcaccgct ctcggcatgc gcggccagca tgctgccgta
     1201 atccatcttg tagacgtgat cgccggccag gatcagcacg aattccgggg cgtggcggtg
     1261 gatgatgtcg aggttctgca gcaccgcgtt cgcggtgccc tcgtaccacg aggtctcgat
     1321 gcgctgctgt gccggcagca cctcgacgaa ctcgccgagc tccgcgcgca ggaaactcca
     1381 gccgtgctgg atgtgacgga tcagcgaatg cgatttgtac tgcgtgagca ccgcgacctg
     1441 gcggataccg gagttgacgc agttggacag cgtgaagtcg atgatgcgga acttgccgcc
     1501 gaagggcacc gcgggcttgg cgcgccagtc agtcagctgc ttcaggcgcg aaccgcgccc
     1561 cccggcgagg atcagcgcca gcgtgtcgcg cgtcagccgg ctcacgaagc ggggggatcg
     1621 gttgggcgtc atgtcgcgct gctccctggt caggtcggtc cgcatctttg caggcacatc
     1681 acgtgccgtt tgtaacgatt gtcataatcc tatctaatca tgacactttg cagtatccga
     1741 gcagtagcgc cgaattcccc gatgcaccga agtcctgacc tggatcacgc cgcggccgat
     1801 gccgcgctgc tcgcgcgcga ggacgtgcgc cgcctgctcg cctcgcgcct gcacgacccc
     1861 ttcgccctgc tcgggcgcca cggtgaggag atccggctgt ggcgcccctg ggcggtggcc
     1921 gcctgggtcg gcgccgagcg cgtgccggcg cgccgcgtcg ggacaagcgg cctgttcgtg
     1981 ctgcgccacg acggcgcgcc agggccgcat cccgaggtgc actggcgcga cgtggccggc
     2041 gccttgcact gcgacatcga cccctacggt ttcccgccgc aaatcggcga cctcgacctg
     2101 catctcttcg gcgagggccg ccaccgccac gcctaccgca tgctcggcgc gcacccgtgc
     2161 accgtggacg gcatcgcggg tacgcgtttc gccacctggg ccccgaacgc cgagcgtgtc
     2221 agcgtggtcg gggacttcaa ccagtgggac ggccgctgcc acccgatgcg cgtgcgcggc
     2281 gccagcgggg tctgggagct gttccttccg ggcgtcgctc ccggcgcgct gtaccgcttc
     2341 gaactgcgct cgcgccatga cggctcgctg cacctgaagg ccgaccccta cggccagcag
     2401 ttcgagcgcc gcccccacac ggcctcgatc gtcacggcac cccctgccca cgcgtggcag
     2461 gacagcgagt ggatggagcg gcgacgcgaa tgggactggc tgcatgcgcc ggtgtcggtc
     2521 tacgagctgc atctgggctc gtggcagcgg cgcgaggacg gcgagttcct cgattatcgc
     2581 gagctcgcgc accgcgtcgc cgagcatgca atctggctgg gcttcacgca catcgagctg
     2641 atgccggtca cggagcaccc gctcgacgcc tcctggggct accaggccac cggctacttc
     2701 gcgccgacca gccgcttcgg cagccccgac gatttccgct acttcgtgga ccattgccac
     2761 cagcgcggca ttggcgttct gctcgactgg gtgccggcgc atttcccgcg cgatgcgcac
     2821 gcgctggcgc gctttgacgg cacgccgctc tacgagcacg ccgaccccag gcgcggcgag
     2881 caccgcgact ggggcacgct gatctacgat ttcgggcgca acgaggtgcg caacttcctg
     2941 ctcgccagcg ccaactgctg gctggaggaa ttccacatcg acggcctgcg cgtcgatgcg
     3001 gtcgcctcga tgctctacct cgactactcg cgcgagcccg gcgactggac gccgaacgtg
     3061 cacggcgggc gcgagaacct cgaggcgatg cagttcctgc gcgaactgaa cgaactcacg
     3121 cacggcgagc atccgggcac gctgacgatt gccgaggaat ccacggcgtg gccgcaggtc
     3181 acgcgcccgg tgtggctcgg cggcctcggc ttctcgatga aatggaacat gggctggatg
     3241 cacgacaccc tgggctacat ggcccacgac ccggtgcacc gcgtctacca ccacccggag
     3301 ctcaccttcg gcctgcttta cgcgttcacc gagaacttcc tgctgccctt ctcgcacgac
     3361 gaggtcgtgc acggcaaggg ctcgatgctc ggcaagatgc cgggcgacga ctggcagcgt
     3421 ttcgcgaacc tgcgcctgct gtacctgtac cagtacacct atccgggcgc caagctgatg
     3481 ttcatgggcg gcgagttcgc ccaggggcgc gagtggaacc acgcccaggc gctcgaatgg
     3541 gcggcgctcg agggcgagcg cgaacgcggc gtgttcgcgc tggtgcgcga cctgaaccac
     3601 ctgtaccgcg atcagttcgc gctgcacggg cacggtttcg agcaacaggg cttcgaatgg
     3661 atcgattgcc acgacgccac gcaatcggtg atcagctacc tgcgccgcgc cggcgacgag
     3721 tgctgcgtgg tggtgctgaa cttcacgccg gtgccgcgcc ggggctatcg catcggcgtg
     3781 cccgagagcg gcagttatcg cgagctgctg aacaccgatt cgcgctatta cgccggcagc
     3841 gatctcggca acggcgcgcc gctgaccgcc gtcgccgagc cgctgatggg gcgcccgttc
     3901 tcgctcggca tcacgctgcc gccactggcg ggcctcgtgc tcaggcgcga agggggttga
     3961 gacgatgcgc gtgctgttcg tcacctccga ggcgcacccg ctgatgaaga ccggcgggct
     4021 cgccgatgtc tcggccagcc tgccgcgcgc gctgcagtca ctgggccacc aggtgacgct
     4081 gctgatgccg gcctaccgcg acgcgctcgc ggcggccggt ccgctgggcg tgaagctcgc
     4141 cgccacgcgc gacgtggacg ggcgccatgt gcgcctgctc cagacgcggc tgcccggcag
     4201 ccgcaacaag gtgtggctgc tcgactgccc ggcgctgttc gaccgtcccg gcaaccctta
     4261 ccacgatgcc agcggcctcg cctgggccga caacgccgag cgcttcatgc tgctgtgtcg
     4321 cgtgggcgcg ctgctcgccg acgatgcatt cggactgggt tggcgccccg aagtggtgca
     4381 ctgcaacgac tggcagagcg ccctggtgcc gttgctgctg cacgcgcgcc acccgcggcc
     4441 ggcactggtc ttcacgatcc acaacctcgc ctaccagggc ctgttccccc gcgaggaatt
     4501 catgcgcctg ggcatcgcgc cggattactg gcacccggat gcgctggagt tccatgggca
     4561 gctgagcttc atcaagggtg gcatcgtgtt cgccgaccgt gtgaccacgg tgagccccag
     4621 ctacgcgcgc gagatccagg gcgcggaatt cggccacgga ctcgacgggc tgctgcgcca
     4681 ccgcgcgccg gtgctgagcg ggatcctgaa cggcatcgac accacggcgt ggaatccctc
     4741 gcgcgacccg gcgctcgccc tcaactacgg cgcgaccacg ctggcgcgca agcgacagaa
     4801 caagatcgcg ctgcaggccg aaacgggcct cgacaccgat cccgccacac cgctgttcgc
     4861 gttcatcggc cgcctcgccg agcagaaggg catcgacctg ctgctgcagg cgctgcccgt
     4921 gctgctggaa cacccggcgc aggtcgtgat gctcggcagt ggtgatgcgg cctacgagac
     4981 ggcgctcgcg gcgctcgccg acgcgcattc ggggcgcttc gcgctgcgca tcggctacga
     5041 cgaggtgctg gcgcatcgca tcgaagccgg tgccgacatc ttcctgatgc cctcgcgctt
     5101 cgagcc
//
LOCUS       DAORUQ010000210         5101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20067_length_5101_cov_6.271502, whole genome shotgun sequence.
ACCESSION   DAORUQ010000210 DAORUQ010000000
VERSION     DAORUQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20067_length_5101_cov_6.271502"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..621
                     /locus_tag="PLW13_14060"
     CDS             265..621
                     /locus_tag="PLW13_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71556.1"
                     /translation="MSSAHRIATVNKYVEAFARKDIGLIREIFADNATVEDPVGTDAH
                     KGIAAISAFYEGSFGADARLSLTGPVRCAGNAAAFAFEVHVAGMKIEVIDVFEFDAEG
                     KVVSMKAYWGQENISQ"
     gene            630..1466
                     /locus_tag="PLW13_14065"
     CDS             630..1466
                     /locus_tag="PLW13_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY71557.1"
                     /translation="MQYGPLHAARDGGVVVVTIDSPPLQLVDAAFIGGLLELLPALES
                     DPAPRVVVFRSADPDYFLMHGDVEYLLGAAPAHGEPVSEPNIAAATFDRLHHAPFLTI
                     GVLDGIARGGGCEFLSALDLRIGSPRAVIGQPEAAMGILPGAGGTVRWSRLVGRARAL
                     ELLLTGRDIHAQEALALGWLQALVPRERLDEEAASLAARIAHLPAAGIAAVKQVVDAA
                     LGAERGALVAESDALERLMAAGQHRAPMRRFLAAGGQTRAGELTGVTALLDAMLGIER
                     DP"
     gene            1544..2668
                     /locus_tag="PLW13_14070"
     CDS             1544..2668
                     /locus_tag="PLW13_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71558.1"
                     /translation="MGRSLWLALLILVSGCTNAPGRPVSGNLTPRDVLAGTPIIGTQA
                     PALLPAEDLLALDQPMRAFLARHIDMSAGATQRLEQLVTALDDERSFGLRYTDATRTA
                     PETFRSRDGNCLALTNLFVALARGARLDVSYQEVDIAPDWQMQGDMFLFYRHINAVVD
                     LGAAGRRTVDFSSEQPTRSENRRRVSDARARAHYHGNLAVALMQADDPGNAFLSLRRG
                     LQADDSFSPLWTNLGALYLRVDATAHAEASYLEALRRRRTDMVAISGLARVYERIGDE
                     RRAARYHELAANFRNENPFFRVQQARTELLAGNPERALRHLDFAIARRPGDASFLRLR
                     ASVLLQLGDTERARADLQQAAAIARAEQIPVASHPARDGA"
     gene            complement(2665..3585)
                     /locus_tag="PLW13_14075"
     CDS             complement(2665..3585)
                     /locus_tag="PLW13_14075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY71559.1"
                     /translation="MLHFLPSILRGIIASLILATNTLVLCWPLFIGALFKAALPFPAA
                     RRVINRAMHWCAETWIDINKFWMNLVQRTRWDVRGLEGVDSGHSYLVTSNHQSWVDIL
                     VLQYLLNRRAPFLKFFLKQELIWVPVIGLCWWALEFPFMKRFSREYLERYPEKRGQDM
                     LTTRKACERFRLSHVCVFNFLEGTRNTPAKHAAQGSPFRYLLKPKAGGIAFVLDAMGD
                     QLSAMLNVTIHYPGGVPGFWDLLSGRLGQVVVRLEQVEIPGDFIGRNYDRDETYRFAF
                     QQWVNRLWEEKDALLSRLHAEYAVRVNGSS"
     gene            complement(3735..>5101)
                     /locus_tag="PLW13_14080"
     CDS             complement(3735..>5101)
                     /locus_tag="PLW13_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY71560.1"
                     /translation="ERPISNFLPQPDGGYLKLGGGLERTQREFRRFSAHDAGILQAYY
                     AALEAVADVLRELALKAPPNLGDGLTAILDAARQGRRVAGLALATQRDMLDLFAKSAR
                     TFLDGWFESEAVKAAFGFDAVVGNYASPDTPGLAYVLLHHVFGEVNGNKGAWGHVLGG
                     MGAITQAMATIVQGMGVQISLEAAVAQVIVDAGRAVGVRLESGEEVMADMVIANVGPK
                     LLYERMIAPSELPADFLRRIRAFKAASGTLRMNVALSRLPNFSCLPGEGEHLQSGIII
                     APTLDYMDQAFLDARQSGMSKAPIVEMLIPSIVDDSLAPPGGHVASLFCQQFAPVLPG
                     GRSWDEEREAAADRVIATVEAYAPGFGASVLGRQILSPLDLERTFGLVGGDIMHGNMS
                     LDQLWSARPVLGHGAYRGPLKGLYMCGAGTHPGGGVTGAPGHNAAHRVLADRSLLGRL
                     LRRG"
BASE COUNT          848 a         1820 c         1700 g          733 t
ORIGIN      
        1 ggcgcatcgt gcgcagctgt gccagcagcg catcgtcgat gaaatgggtc ttgagcagaa
       61 cggcgatcac gggattcaga tgggctccga cgacccggcc gcaagcacgg ccggtgcagg
      121 cggcaaggat aatcgcgctg ccgccagcag gccagccgcg acagcgctgc actgtcgccg
      181 ggcacggcag cgctcgccga ccgatggcac accctgctca tttccgctat aaagccgcag
      241 ccgcactaca acaacggaga aaccatgagc agcgcacaca gaatcgcgac cgtgaacaag
      301 tacgtggagg cctttgcccg caaggacatc ggcctgatcc gcgagatatt cgccgacaac
      361 gccaccgtcg aggacccggt cggaaccgat gcgcacaagg gcatcgcggc aatcagcgcg
      421 ttctacgagg gcagcttcgg cgccgatgcc cggctgagcc tcaccgggcc cgtgcgctgc
      481 gccggcaacg ccgcggcgtt tgccttcgaa gtgcatgtgg ccggcatgaa gattgaagtg
      541 atcgacgtgt tcgagttcga cgccgagggc aaggtcgtgt cgatgaaggc ctactggggc
      601 caggaaaaca tctcgcagta ggcccgccga tgcaatacgg gccgctgcat gccgccaggg
      661 acggcggtgt cgtcgtcgtc acgatcgact cgccgccgct gcaactcgtc gatgctgcgt
      721 tcatcggcgg cctgctcgaa ttgctgcccg cgctggagtc cgacccggcg ccgagggtgg
      781 tggtgttccg cagcgcggat cccgactact tcctgatgca cggtgatgtc gagtatctgc
      841 tgggcgcagc gccggcccac ggcgagccgg tcagcgagcc caacatcgcc gccgcgacct
      901 tcgaccggct gcaccacgcc ccgttcctga cgatcggcgt gctcgacggc atcgcccgcg
      961 gtggcggctg cgagttcctg tccgcgctgg acctgcgcat cggctccccg cgcgccgtga
     1021 tcggacaacc ggaggccgcc atgggcatcc tgcccggcgc cggcggcacc gtgcgctggt
     1081 cgcgcctggt gggtcgcgcc cgggccctcg agctcctcct caccgggcgc gacatccacg
     1141 cgcaggaggc gctcgcgctg ggctggctgc aggcgctggt cccgcgcgaa cgcctcgacg
     1201 aggaagcggc gtcgctggcc gcgcggattg ctcacctgcc cgcggccggc atcgcggcgg
     1261 tgaaacaggt ggtggatgcc gcgctcgggg ccgagcgcgg cgccctcgtc gccgagagcg
     1321 acgcactcga gcgcctgatg gccgcgggcc agcaccgcgc ccccatgcgc cgcttcctcg
     1381 ccgccggcgg acagacccgc gcgggcgaac tgacgggcgt gaccgcgctc ctcgacgcca
     1441 tgctcgggat cgagcgcgac ccgtaggttc gccccgcggc accggcgctg gaaatccgcg
     1501 ccgatgcggc gtagattccg gccgggaccg cgcaggaggc cgtatgggca ggtcgttgtg
     1561 gctggctctg ctgatactcg tgtccggctg cacgaatgca cccggacgtc ccgtcagcgg
     1621 caacctcacg ccgcgcgacg tgctggcggg caccccgatc atcggcacgc aggccccggc
     1681 gctcctgcct gccgaggatc tgctcgcact cgaccagccg atgcgcgcct tcctggcacg
     1741 gcacatcgac atgagcgccg gcgccacgca acgcctggaa cagctcgtga cggcgctcga
     1801 cgacgagcgc agtttcgggc tgcgctacac cgatgcgaca cgcactgcgc ccgagacctt
     1861 ccgcagccgc gacggcaatt gcctggcgct caccaacctc ttcgtcgcgc tggcgcgcgg
     1921 cgcgcgactc gacgtcagct accaggaggt ggacattgcg cccgactggc aaatgcaggg
     1981 cgatatgttc cttttctacc ggcacatcaa cgcagtggtc gatctgggcg cggcggggcg
     2041 gcgcaccgtc gatttcagca gtgagcagcc gacgcgcagc gagaaccgca ggcgcgtttc
     2101 cgacgcgcgc gcccgggcgc actaccacgg caatcttgcg gtcgcgctga tgcaggcgga
     2161 cgacccgggc aatgcattcc tgagcctgcg ccgcggactg caggccgatg acagcttctc
     2221 gccgctgtgg accaacctgg gggcgctcta tctgcgggtc gacgccacgg cacacgccga
     2281 agcgagctat ctcgaggccc tgcgccgcag gcgcaccgac atggtagcca tcagcggact
     2341 ggcgcgcgtc tatgagcgca tcggcgacga gcgccgcgcg gcgcgctatc acgagctggc
     2401 cgcaaacttc cgcaacgaga atcctttctt ccgcgtccag caggcgcgca cggagttgct
     2461 ggccgggaat cccgagcgcg cgctgcggca cctggatttc gcgattgccc gccgaccggg
     2521 cgacgcctcg ttcctgcgcc tgcgtgccag cgtcctgctg caactgggcg acaccgaacg
     2581 cgcacgtgcc gacctgcagc aggctgctgc gatcgcgcgc gccgaacaga ttcccgtggc
     2641 atcccacccc gcccgggatg gcgcctagga ggatccgttc acccgcaccg catactctgc
     2701 gtgcaaccgt gacagcagcg cgtccttctc ctcccacagg cgattcaccc actgctggaa
     2761 cgcgaaccgg taggtctcgt cccggtcgta gttgcgaccg atgaaatcgc cgggaatctc
     2821 cacctgctcc aggcgcacca cgacctgccc gagcctgccc gacagcaggt cccagaaccc
     2881 gggcacgccg cccgggtagt gaatcgtcac gttcagcatg gcgctcaact gatcgcccat
     2941 ggcgtccagc acgaacgcga tgccgcccgc cttcggcttc agcaggtagc gaaacggcga
     3001 gccctgggcg gcgtgcttgg ccggggtatt gcgcgttccc tcgagaaagt tgaacacgca
     3061 gacatggctg agccggaagc gctcgcaggc cttgcgcgtg gtgagcatgt cctgcccgcg
     3121 cttctccggg tagcgctcca ggtattcgcg tgagaaccgc ttcatgaacg gaaactcgag
     3181 cgcccaccag cacagcccga tcaccgggac ccagatcagt tcctgcttga ggaagaattt
     3241 caggaatggc gcacggcggt tcagcaggta ctgcagcacg aggatgtcga cccagctctg
     3301 gtgattggag gtcacgaggt aggaatgccc ggaatccacg ccctccaggc cgcgcacgtc
     3361 ccagcgcgtg cgctgcacga ggttcatcca gaacttgttg atatcgatcc aggtttccgc
     3421 gcaccagtgc atcgcgcggt tgatcacgcg ccgggccgcc gggaacggca gtgccgcctt
     3481 gaacagcgcg ccgatgaaca gcggccagca cagtaccagg gtgttggtgg caagaattaa
     3541 cgaggcaatg atgccccgca ggatggaagg caggaagtgc agcatcgggg cgaggcgctc
     3601 gggcggattt gccgcgggat tctagcgcga accccggcgg tacagaatcg ccgcgacgcc
     3661 tggcgcgggc acgcgcgccc ggcaaaccgc gccggcgcgc gaacgttgca gcgcccggca
     3721 cgcggccccg ccgctcaacc ccggcgcagc aagcgcccga gcagcgagcg gtcggcgagg
     3781 acccggtgcg cggcattgtg ccccggcgcg ccggtgacgc caccgccggg atgcgtgccc
     3841 gcgccgcaca tgtacaggcc cttcagcggc ccgcgatacg ccccatggcc gagcaccggg
     3901 cgcgcggacc acaactgatc gagcgacatg ttgccgtgca tgatgtcgcc gccgacgagc
     3961 ccgaaggtgc gctccaggtc gagcggcgaa aggatctggc gccccagcac cgaggccccg
     4021 aaacccggcg catacgcctc gacggtggcg atgacacgat cggccgccgc ctcgcgctcc
     4081 tcgtcccagc tgcgcccgcc cggcagcacc ggcgcgaact gctggcagaa caggctggcg
     4141 acgtgcccac cgggcggcgc gagcgaatcg tcgacgatcg aggggatcag catctcgacg
     4201 atcggcgcct tcgacatgcc ggattgcctg gcatcgagga aggcctggtc catgtaatcg
     4261 agcgtcgggg cgatgatgat gcccgactgc agatgctcgc cctcgcccgg caggcagctg
     4321 aagttcggca gccgcgacag cgccacgttc atgcgcaagg tgcccgaggc ggccttgaac
     4381 gcgcggatgc ggcgcaggaa atccgcgggc agctcggacg gcgcgatcat gcgctcgtac
     4441 agcagcttcg gccccacgtt cgcgatgacc atgtcggcca tcacctcctc gccgctctcc
     4501 agccgtacac cgacagcgcg gccggcatcg acgatcacct gcgcgaccgc cgcttccagg
     4561 ctgatctgca cgcccatgcc ctgcacgatc gtcgccatcg cctgcgtgat cgcccccatg
     4621 ccgccgagca catggcccca ggcgcccttg ttgccgttca cctcgccgaa gacgtggtgc
     4681 agcaatacat aggccaagcc cggcgtgtcg gggctcgcat agttgccgac cacggcatcg
     4741 aagccgaagg ccgccttgac cgcctcgctc tcgaaccagc cgtcgaggaa tgtacgcgcc
     4801 gatttcgcga acagatccag catgtcgcgc tgcgtcgcga gcgcgagtcc ggccacgcga
     4861 cggccctggc gcgcggcatc gaggatcgcc gtcagcccgt cgccgaggtt cggcggcgcc
     4921 ttcaacgcga gctcgcgcag cacgtcggcg accgcttcca gcgccgcgta gtacgcctgc
     4981 aggatcccgg cgtcgtgcgc cgagaagcgg cgaaattccc gttgcgtgcg ctccagcccg
     5041 ccgccgagct tcaggtagcc gccgtcgggc tggggcagga aattcgagat cggccgctcg
     5101 a
//
LOCUS       DAORUQ010000211         5101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20068_length_5101_cov_5.612366, whole genome shotgun sequence.
ACCESSION   DAORUQ010000211 DAORUQ010000000
VERSION     DAORUQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20068_length_5101_cov_5.612366"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2955)
                     /locus_tag="PLW13_14085"
     CDS             complement(<1..2955)
                     /locus_tag="PLW13_14085"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HQY71561.1"
                     /translation="MTPGTLPRLIAFYLPQFHPIPQNDAWWGKGFTEWTNVTQARPLF
                     PGHQQPRLPADLGFYDLRVPEVREAQAELAGRHGIHGFCYYYYWFKDSVQLLDRPLRE
                     MLASGAPDFPFCLCWANENWTRRWDGREQDVLMAQDHSRENNLAFIRSIVPYLQDPRY
                     IRVDGRPVLLIYRAALIEDLAATVAAWRQELARHGLPAPYLVAAETFGQSGAQAVAAG
                     FDAACEFPPHGSTQEAALPAHAVGLDASFTGALSDYARVASGFRERPLPDYPLHRGVM
                     LAWDNTARRGNSAHIAVNFSVESYHAWLRATVQSTQRAFRGERQLLFVNAWNEWSEGA
                     YLEPDQLYGHACLEATRAALLDQPLAPAARTVARRLEPVRLAPQEASPDPDTGADSRG
                     ASLPARERGGTLKLVAIAMIGNEADVVEAFVRDNLDYVDHLLIAEHNSLDGTRDILQA
                     LVEEGLPLTVTRIETAAFRQAAVTNALLADALARFEPDWVIPLDADEFLDADGRVALE
                     AELARLADSHARLRWVQHVPAGGDDAAEQHPARRIRHRYAYTPPDPGLNPYVWKLALN
                     CRLIRPYLDRYDLEKGSHRVVFKGVREPSSQPVTTLQHTVLRHYPVRSFEQLSLKAGL
                     STLQGRLAGRNELGGTHVSRLYQQILAGKHELAAMQRSVREYLDTGRHGPDDLAHTAM
                     IVDAKQRTSALRHGRLRQPATVAFLRWIERQGTAAVGQPGAVDAAGSALSTRFDYCPW
                     LFWGEASADARAQQLAFQAHWAAADRLRCGSRCFISPQAGFVPGKVTLGDQCYIAAQA
                     YVTDQLNAGSHCTINPFAVVRGTVTMGDHVRVGAHASILGFNHNHARLDRPIHAQGLS
                     SKGIRIGDDVWIGSGALLLDGVSVGSHCIVAAGAVVTRDVPDWAVVAGNPARVLRDRR
                     ERASAATRHPSLARTLADIGRRAAAQWPAIIARSEVRGGEEIGYTDIPGTPPASIRPL
                     ADAIEIAAAFGAL"
     gene            complement(2984..4201)
                     /locus_tag="PLW13_14090"
     CDS             complement(2984..4201)
                     /locus_tag="PLW13_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY71562.1"
                     /translation="MQRALTAQMEGKLEVAQALYREALQRAPANFDALHMLGVVRLQC
                     GDPEEAVRHIVRAIRLGTVDYPPIYDNLVLCLQNIARRRGSLAELLDAAAPAPDAPRI
                     RFAQDVAAFAAGPPLVSALLRCADDDVARFAPGLESLCRQTHRRLEFVLGTAGPGTRA
                     FEGMAVLLADRAFALRHLPGEEAAAPPTLDACAAQARGRYVVALEPGDEYDADHIELL
                     ARLLEACGSRWGFSGVRFADAQGRRLRYGDDARADELMRGLDDIHAERSLTAAFLQFN
                     HAIAAGNLLVEKRLWEELGGFREDSPDPAWDFCVRASLVEAPAILFEPKYTHRLRGGA
                     HAEAQAERMRALWLAEVRRACADRPQALQRVFAARQLREWRLLEIGRGRELAPVTLLA
                     LAEDLIAARDAHD"
     gene            4558..4938
                     /locus_tag="PLW13_14095"
     CDS             4558..4938
                     /locus_tag="PLW13_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQY71563.1"
                     /translation="MHPPSFATAECIKLVTRRRSGAEVATPVWFVLCNDTACLRTATR
                     FGKVKRLGNDPAVRYAPCDWDGNVSGPWLEGRAELIPANDARATEIDRLLEQKYGERR
                     ADMNRLMQAEGMEPVFVAITPTAP"
BASE COUNT          794 a         1797 c         1782 g          728 t
ORIGIN      
        1 cagggcgccg aacgcggcgg cgatctcgat ggcgtcggcc aggggccgga tgctcgcggg
       61 cggcgtgccg ggaatatcgg tgtagccgat ctcctcgccg ccgcggacct cgctgcgcgc
      121 gatgatcgcg ggccactgcg ccgcggcgcg ccggccgata tcggcgagcg tgcgcgcgag
      181 cgaggggtgg cgcgtcgcag cgctcgcgcg ctcgcgccgg tcgcgcagca cccgggccgg
      241 gttgcccgcc accaccgccc agtcgggcac gtcgcgggtc accaccgcgc ccgccgcgac
      301 gatgcagtgg ctgccgacgc tgacgccgtc cagcaacagc gcgcccgagc cgatccacac
      361 atcgtccccg atgcggatgc ctttcgacga caggccctgc gcgtggatgg gccggtcgag
      421 cctggcgtgg ttgtggttga agccgaggat gctcgcgtgc gcgccgacac gcacgtgatc
      481 gcccatggtc accgtgccgc gcaccacggc gaacgggttg atcgtgcagt ggcttccggc
      541 gttcagctga tccgtgacat aggcctgggc cgcgatatag cactggtcac cgagcgttac
      601 cttgccgggc acgaaaccgg cctgcggcga aatgaagcag cgcgatccgc agcgcaggcg
      661 atccgcggcg gcccagtgcg cctggaaggc cagctgttgc gcgcgcgcgt ccgccgaagc
      721 ctcgccccag aacaaccagg ggcagtaatc gaagcgcgtc gacagggcac tgcctgcggc
      781 atccaccgcg cccggctggc ccacggccgc cgtgccctgg cgctcgatcc agcgcaggaa
      841 cgccaccgtc gcgggctggc gcagacgtcc atggcgcaac gcgctcgtgc gttgcttggc
      901 atcgacgatc atcgcggtgt gggcaagatc gtccggaccg tggcgtccgg tatcgagata
      961 ttcccgcacc gagcgttgca tggcggcgag ttcgtgcttt cccgcgagga tctgctggta
     1021 cagtcgggac acgtgcgtgc cgccgagctc gttgcgcccg gcgagccggc cctgcagcgt
     1081 gctgagcccc gccttcaggc tcagctgctc gaaggagcgc accgggtaat ggcgcaacac
     1141 ggtgtgctgc agcgtcgtca cgggctgcga ggacggctcc ctgacgccct tgaacacgac
     1201 gcggtggctg cccttctcga gatcgtagcg atccaggtag ggccggatca ggcggcagtt
     1261 gagcgccagc ttccacacat aggggttcag cccgggatcg ggcggcgtat aggcgtagcg
     1321 gtgccggatg cgccgtgcgg gatgctgttc ggccgcatcg tcgccccccg ccggcacgtg
     1381 ctgcacccag cgcagcctgg cgtggctgtc cgcgagcctc gccagctccg cctccagcgc
     1441 gacgcggcca tcggcgtcga ggaactcgtc ggcatccagc gggatcaccc agtcgggctc
     1501 gaagcgcgcg agcgcgtcgg ccagcagggc attggtcacg gcggcctgcc ggaaggcggc
     1561 ggtctcgatc cgcgtcaccg tcagcggcaa gccctcctcg acgagcgcct gcaggatgtc
     1621 gcgcgtgccg tcgagcgagt tgtgctccgc gatcagcagg tgatcgacgt agtcgagatt
     1681 gtcgcgcacg aatgcctcca ccacgtcggc ctcgttgccg atcatggcga tcgcgaccag
     1741 cttcagcgtg ccgccacgct cgcgcgcggg gagcgacgcg ccgcgtgaat cggcccccgt
     1801 atccgggtcg ggcgatgctt cctgtggcgc gagccggacg ggctcgaggc gccgggcgac
     1861 cgtgcgcgct gccggagcca atggctgatc gagcagcgcc gcgcgggttg cctccaggca
     1921 ggcgtggccg tacagctggt ccggctcgag ataggcgcct tcggaccact cgttccacgc
     1981 gttgacgaac agcagctggc gctcgccccg gaacgcgcgc tgcgtcgact gcaccgtcgc
     2041 gcgcagccag gcgtgatagg actccacgga aaaattcacc gcgatgtgcg ccgagttgcc
     2101 gcgccgcgcg gtgttgtccc aggccagcat gacaccgcga tggagcgggt aatccgggag
     2161 cgggcgttcg cgaaaaccgg atgcgaccct cgcatagtcg ctaagcgcac cggtgaaact
     2221 ggcatccagg ccgacggcgt gcgccggcag cgcggcctcc tgtgtcgagc cgtgcggcgg
     2281 gaattcacag gccgcgtcga agccggcggc caccgcttgc gccccggatt gcccgaaagt
     2341 ttccgccgcc accaggtacg gcgccggcag gccgtggcgc gcgagctcct ggcgccacgc
     2401 cgccaccgtc gcggcgagat cctcgatcag cgccgcgcga tagatcagca gcaccggccg
     2461 gccatcgacg cggatgtaac ggggatcctg caggtacggc acgatgctgc ggatgaacgc
     2521 caggttgttt tcccggctgt ggtcctgcgc catgagcacg tcctgctcgc gaccgtccca
     2581 gcgccgggtc cagttctcgt tggcccagca caggcagaac gggaaatccg gcgctcccga
     2641 cgcgagcatc tcgcgcagcg ggcgatcgag cagctgcacc gagtccttga accagtagta
     2701 gtagtaacag aagccgtgga tgccatggcg acccgccagt tccgcctgcg cctcgcgcac
     2761 ctcgggaacc cgcaggtcgt agaagccgag atcggcgggc agtcgcggct gctgatgacc
     2821 ggggaacagc ggacgcgcct gcgtgacatt ggtccactcc gtgaacccct tgccccacca
     2881 cgcatcgttc tgcgggatcg gatggaactg cggcaggtag aaggcgatca gcctcggcaa
     2941 ggtcccgggt gtcaattacg tgattccgtt tggatggctc ggctcaatca tgtgcatccc
     3001 gcgcggcgat cagatcttcg gcaagggcga gcagcgtgac gggcgccagc tcgcgaccgc
     3061 gaccgatttc cagcagtcgc cattcacgta gctgccgcgc ggcgaaaacc cgctgcagcg
     3121 cttgcggccg gtccgcgcag gcgcgcctga cctcggcgag ccatagcgcc cgcatgcgtt
     3181 ctgcttgcgc ctcggcatga gcaccaccgc gcaaccggtg cgtgtacttc ggctcgaaca
     3241 ggatggcggg cgcctccacc agcgaggcgc gcacgcagaa atcccaggcg ggatcggggc
     3301 tgtcctcgcg aaagcccccc agctcttccc acaaacgctt ctcgaccagc aggttgccgg
     3361 cggcaatcgc gtggttgaat tgcaggaacg cggccgtgag cgatcgttcg gcgtgaatat
     3421 catcgagccc gcgcatcaac tcatcggcgc gcgcgtcgtc gccgtagcgc aggcgccgcc
     3481 cctgcgcgtc ggcgaaccgg acgccgctga agccccagcg gctgccgcaa gcctcgagca
     3541 ggcgcgccag cagttcgata tggtcggcat cgtattcgtc gccgggctcc agcgccacca
     3601 cgtagcgccc gcgtgcctga gcggcgcagg cgtccagcgt cgggggcgcc gcggcctctt
     3661 ccccgggcaa gtgacgcagc gcaaacgcgc gatccgcgag cagcacggcc atgccctcga
     3721 aggcgcgcgt gccgggcccc gcggtgccga gcacgaactc gagacgccga tgcgtctgcc
     3781 ggcacagcga ctccaggccc ggggcgaacc gtgcgacgtc gtcatcggcg cagcgcagca
     3841 gcgccgacac cagcggcggc ccggcggcga aagcggcgac atcctgcgcg aagcggatcc
     3901 gcggcgcgtc gggcgcgggc gccgcggcat cgagcagctc ggccaggctg ccgcgcctgc
     3961 gcgcaatgtt ttgcaggcac agcaccaggt tgtcgtagat cggcgggtaa tccacggtgc
     4021 caagccggat ggcgcgcacg atatggcgga cagcttcctc gggatcgccg cactgcagcc
     4081 tcaccacccc gagcatgtgc agggcgtcga agttggcggg agcccgctgc agcgcctcgc
     4141 ggtacagggc ttgcgcgact tcgagcttgc cctccatctg ggccgtcagt gcccgctgca
     4201 tcagctccgg cagcccggcg accggagcga cgccccccgg cgcctcgatt cccggcgcgc
     4261 cagcaatcgg cgccgggtcg gcgccgcagc aatgcttgta gcgcttgccg ctgccgcagc
     4321 tgcatggctg gtttctgctg atctgggtca ctggtctgag cggattgccg cgtatcccga
     4381 agtcgcctga aattgaaggc gcgagcctac tggaggcgtg cacgagcggc aaggattgca
     4441 gcgggcaaat tccccgcacg acgcgggatg agccccgcct gcgccacggg tacccctgat
     4501 caggcacttt acggataatc gcgacacaca ggtcccacga cacgaccgga gccacccatg
     4561 catcctccct ccttcgccac cgccgaatgc atcaagctgg tgacgcgccg gcgcagcggc
     4621 gccgaggtgg cgacaccggt gtggttcgtg ctgtgcaacg acaccgcctg cctgcgcacg
     4681 gcgacgcgct ttggcaaggt gaagcgcctc ggcaacgatc ccgcagtgcg ctacgcaccc
     4741 tgcgactggg acggcaacgt gagcggcccg tggctggagg gacgcgcgga gctgattccc
     4801 gcgaacgacg cgcgcgccac cgagatcgac cggctgctgg agcagaagta cggcgagcgg
     4861 cgcgccgaca tgaaccgcct gatgcaggcc gaaggcatgg agccggtgtt cgttgcgatc
     4921 acgccgacgg cgccgtgagc cccgttgcgc gcgcgacgcc gcgcgccagc tcccgggctc
     4981 tcagccgaag ccctcgcccg ggggcagctc cgccaggcgg acgagccggc tgtcgtcgac
     5041 cgctgcctgg cggtgcacga cggcgagccg gtagccaggg tcggtcacca tctccagaaa
     5101 c
//
LOCUS       DAORUQ010000212         5022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20548_length_5022_cov_6.259513, whole genome shotgun sequence.
ACCESSION   DAORUQ010000212 DAORUQ010000000
VERSION     DAORUQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20548_length_5022_cov_6.259513"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLW13_14100"
     CDS             complement(<1..219)
                     /locus_tag="PLW13_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71564.1"
                     /translation="MAQKKKLADVVAQTIKARPTPYEIRDTGNGSVKGLLLRVQPSGH
                     ATYYVELARGVREKIEPKEITLTAARTKA"
     gene            665..1087
                     /gene="mscL"
                     /locus_tag="PLW13_14105"
     CDS             665..1087
                     /gene="mscL"
                     /locus_tag="PLW13_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQY71565.1"
                     /translation="MSMLKEFKEFAVKGNVVDMAVGIIIGGAFGTIVQSLVKDVLMPP
                     IGLLLGGVDFSGLFLVLKGEGEFRSIADAASAGAVTINYGVFINNIISFLIVAFAVFL
                     LIKGINKLKREEQAAPAEPPPTPEDVLLLREIRDSLKK"
     gene            complement(1182..1718)
                     /locus_tag="PLW13_14110"
     CDS             complement(1182..1718)
                     /locus_tag="PLW13_14110"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQY71566.1"
                     /translation="MQKRSLALGKVYGLLEPGPVLLLSTCHKGRANVMAMSWHTMMDF
                     EPPLVGCVVSARDFSFAALKATRECVLNIPAAELAAKVVACGNSSGRRVDKFAAFGLT
                     AAPAKLVASPLVAECWANLECRVLDTRLVNRYNFFVLEVVKAWIDPAVKDPRTVHHRG
                     RGVFMVAGDSIRLPSRAK"
     gene            complement(1745..2095)
                     /locus_tag="PLW13_14115"
     CDS             complement(1745..2095)
                     /locus_tag="PLW13_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HQY71567.1"
                     /translation="MAWLLTKYAVTAAVVVLVSEFARRSDRLGGLIAALPLVTVLALI
                     WLHVEQQPEQKIANLARYTFWYAVPTLPMLLVFPALLPRLGFWPTLLASVLVALACFA
                     AFALLVRRFGIELL"
     gene            2314..2979
                     /locus_tag="PLW13_14120"
     CDS             2314..2979
                     /locus_tag="PLW13_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY71568.1"
                     /translation="MRVLVVEDSERLRGAISAGLEAAGLAIDMAADGRDALPYLQSAS
                     YAVIVLDIMMPRMDGLALLREIRRRRLPVRVLVLSARDAVADRVGALDIGADDYLVKP
                     FSFDELRARVLALGRRAREEPSALIELDGLELDTARRVARGPGGAITLTPKEYALLET
                     LVRHSGRVLSRAQLFEGIYASGSESSDKVIEVLMSNLRGKLARAGLPELIQTRRGFGY
                     VAG"
     gene            2966..4366
                     /locus_tag="PLW13_14125"
     CDS             2966..4366
                     /locus_tag="PLW13_14125"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQY71569.1"
                     /translation="MSPAEALTLRRRVALGVALAVAVALGGLFLLIDTLVDRELYRRF
                     DAGLSARANAIAGYLGARVEGAGEISLWMPEFREEGHTDYFQAWDARSRVIARSASSQ
                     SVDLPRPDGVTSARFLHYDQRLPDGHRGRAVARVFPLAPGDERGSLTLVVAEEREQID
                     ALEARIHLIFVSGVMLALAATLLLASSGVRLALAPLDRIADRIARIRPGAPREPIVEP
                     GLPAELRPVAQKFDQVVDQLMETLERERRFAQDLAHELRTPVAELRAIAEISLQATEP
                     ERLRGALAELSVLGAEMDRTVEALLTLARFDAGLEVGAAEPLELVALLRESAARFDET
                     VSARGVTFAWALPAECWVQADAAMLERLLATLCANAMDHAPPGSTVRVRLRPDPAPVL
                     EFANPAPQLAEEDLPRLGARFFRAPNGEAGSGGHHAGLGLALARALARAHGLRLDFAL
                     RDGELVIRIDGFVPLP"
     gene            4439..>5022
                     /locus_tag="PLW13_14130"
     CDS             4439..>5022
                     /locus_tag="PLW13_14130"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY71570.1"
                     /translation="MPSPSRTFLCAALLAATLTTAAEAAPAALAISPAQAQAMGLEFA
                     PAVAADWVRVARLPARVELDASVRRLVAARHPGTVAGVYVREGEDVLAGQLLLSLQSP
                     EWADAVALAQANTARLTQAGQAETRARALLEAGVIARRELESAQAELAALRATVAGDA
                     ARTAGARIGRDGSVELLAPSGGRVLRRPHDPGHAV"
BASE COUNT          802 a         1723 c         1698 g          799 t
ORIGIN      
        1 tgccttggtc cgcgcagcag taagcgtgat ctccttcggc tcgatcttct ctcgcacgcc
       61 gcgggccaac tcgacgtagt aggtagcatg gccgcttggc tgcacgcgaa gcagtagccc
      121 tttgacggag ccattgccgg tatctctgat ctcgtacggt gtcggccgcg ccttgatggt
      181 ctgcgcaacg acgtccgcca gcttcttttt ctgcgccatg cacccgtccc agaatctgca
      241 cccacgaact gaataagtgc attgtaagtg caaatcagta tgtcacggac acactttcag
      301 atacacgcac gaattcaagc gcatgattac aaaagactat tttcggcaag tgattgatcg
      361 aaaaatcgga gaattcgccc tccggaggca gaggtcaggg gttcgaatcc cttcgggcgc
      421 gccatattct gaaggcttgg catggtcgca agcctccccc ctcgggctcc gccaatgccc
      481 ggctgctgct ggcgagcttc ttcagaattg ccggcgtctc gaatcgatca gcgtccagtt
      541 tctttcagtc gagcggctgc cgccaccccc ggttatacat ccgacgcttt cttcagctcc
      601 tgctgttaca ctcccgacga ccgggatatc ccggggtgac aaacaacaaa cggagagtgt
      661 tgccatgagc atgctgaagg agttcaagga gttcgccgtc aagggcaacg tggtggacat
      721 ggccgtgggc atcatcatcg gtggcgcctt cgggaccatc gtgcagagcc tggtgaagga
      781 tgtgctgatg ccgccgatcg gcctgctgct gggcggggtg gatttctccg gcctgttcct
      841 ggtgctgaaa ggcgagggcg agttcaggag catcgccgac gccgcctctg ccggcgcggt
      901 caccatcaac tacggcgtat tcatcaacaa catcatcagc ttccttatcg tcgccttcgc
      961 ggtattcctg ctgatcaagg gcatcaacaa gctcaagcgc gaggagcagg cggcacccgc
     1021 ggagccgccg cccaccccgg aagacgtgct gctgctgcgc gagatccgcg acagtctgaa
     1081 gaagtagggc aacccctgaa gagcccggca ccaagccggg ttcttcgctt tcggcaggac
     1141 cagatcccgt tgcccggcgc acgggcccgc cacatcggcg cctatttcgc ccgcgacggc
     1201 agcctgatcg aatcgcccgc gaccatgaac accccgcggc cgcggtggtg caccgtgcgc
     1261 gggtccttca cggccgggtc gatccaggcc ttcacgacct ccagcacgaa gaagttgtag
     1321 cggttcacca ggcgcgtatc gagcacgcgg cactccaggt tcgcccagca ctcggccacc
     1381 agcggcgacg cgaccagctt cgcgggggcc gccgtcaggc cgaaggccgc gaacttgtcc
     1441 acgcgacgcc cggaactgtt gccgcaggcc accaccttcg ccgcgagctc cgcggccggg
     1501 atgttgagca cgcattcgcg cgtggccttc agcgccgcga agctgaagtc gcgagcgctg
     1561 accacgcagc cgaccagcgg cggctcgaag tccatcatcg tgtgccagga catcgccatc
     1621 acgttggcgc gacccttgtg gcacgtggat aaaagaagga ccgggccggg ttcgagcagg
     1681 ccgtagacct tgccgagggc cagcgatcgc ttttgcatgg ttggtttacc ctcgatgagc
     1741 cggactacag caattcgata ccgaaacgcc gcaccagcag cgcgaacgcg gcgaagcagg
     1801 cgagcgcgac gagcacgctg gccagcagcg tgggccagaa ccccaggcgc ggcagcaacg
     1861 ccgggaacac cagcaacatg ggcagcgtgg gcacggcgta ccagaaggtg taccgggcga
     1921 ggttcgcgat cttctgctcg ggctgctgct ccacgtgcaa ccagatcagc gcgagcaccg
     1981 tgaccagcgg cagcgcggcg atcaggccgc cgagacggtc gctacgcctg gcgaattcgg
     2041 aaacgagcac caccacggcg gcggtaacgg cgtatttggt cagcaaccag gccacggcga
     2101 ttcctgggta acgggatgga tacccgcatc ctacggcctg accaggtcga acgaaacagc
     2161 gggactgccg ccgcgccggt tgcggtatgc tctgcgcttc ctgtagtcat gcgcggtgcc
     2221 tgcgcccgtg aacaccatcg ctgccactgc cgccacgcaa cgcccgcgca tgacgccggg
     2281 cggccactcg cccgcgcccg cgtaaacaac accatgcgcg tgctggtcgt cgaggattcg
     2341 gaacgccttc gcggcgccat ctccgcgggg ctggaagcag cggggctcgc gatcgacatg
     2401 gcggcggacg ggcgcgacgc actgccctac ctgcaatcgg cgtcctacgc cgtgatcgtg
     2461 ctcgacataa tgatgccgcg catggatggt cttgcgctgt tgcgcgagat ccgccggcgc
     2521 cggttgccgg tacgcgtgct ggtgctgtcg gcgcgcgacg cagtcgccga ccgcgtcggc
     2581 gcactcgaca tcggcgccga cgactacctg gtgaagccct tctccttcga cgaactgcgc
     2641 gcgcgcgtgc tggcgctggg ccggcgtgcg cgggaagagc cgtcggcgct gatcgagctg
     2701 gatgggctcg agctcgacac ggcgcggcgc gtcgcgcgcg gaccgggcgg cgcgatcacg
     2761 ctgacgccga aggagtacgc gctgctcgag acgctggtac gtcactccgg acgcgtactg
     2821 agccgcgcac agctcttcga gggcatctat gccagcggca gcgagtcctc cgacaaggtg
     2881 atcgaagtgc tgatgagcaa cctgcgcggc aagctcgcgc gcgcaggact ccccgaactc
     2941 atccagacgc ggcgcggctt cggctatgtc gccggctgaa gccctgacac tcaggcggcg
     3001 cgtcgcactg ggtgtggcgc tcgcggtcgc ggtggcgctc ggcggcctgt tcctgctgat
     3061 cgatacactg gtggatcgcg agctttaccg gcgcttcgat gccggcctct cggcacgcgc
     3121 caacgcgatc gcaggctacc tcggcgcgcg cgtcgagggt gccggcgaga tctcgctctg
     3181 gatgccggaa tttcgcgagg aaggacatac ggattacttc caggcctggg acgcgcgctc
     3241 gcgcgtgatc gcgcgctccg catcgagcca gtcggtggac ctgccccggc ccgacggggt
     3301 cacgagcgcg cgcttcctcc attacgacca gcggcttccc gacggccacc gcggccgcgc
     3361 cgtggcgcgc gtgttcccgc tcgctcccgg cgacgaacgc ggcagcctga cgctggtagt
     3421 ggcagaggag cgcgagcaga tcgacgcgct cgaggctcgc atccacctga tcttcgtgtc
     3481 cggggtgatg ctcgcgctcg cggccacgct gctgctggca tcgagcgggg tgcgcctggc
     3541 gctggcgccg ctggaccgca tcgccgatcg catcgcgcgc atccgtcccg gggcgccgcg
     3601 cgagccgatc gtcgagcccg ggctgccggc ggaactgcgc ccggtggcgc agaagttcga
     3661 ccaggtcgtc gaccagctga tggagactct ggagcgcgag cgccgcttcg cccaggatct
     3721 cgcgcacgag ttgcgcacgc ccgtcgcgga gcttcgcgcc atcgccgaga tcagcctgca
     3781 ggccactgaa ccggagcgct tgcgcggggc gctcgcggag ctcagcgtgc tgggtgccga
     3841 gatggaccgc accgtggagg cactgctcac gctggcacgc ttcgacgccg ggctcgaggt
     3901 cggcgccgcg gagccgctgg agctggtcgc gttgctgcgc gagagcgccg cgcgcttcga
     3961 cgagactgtc agcgcgcgcg gagtcacgtt cgcttgggcg ctgcccgccg aatgctgggt
     4021 gcaggcggat gccgccatgc tcgaacgcct gctcgccacg ttgtgcgcca acgcaatgga
     4081 tcacgcccca ccgggcagta ccgtgcgcgt gcgattgcgc cccgatccgg ccccggtgct
     4141 cgagtttgcc aatccggcac cgcaactggc ggaagaagac ctgccccgcc tcggcgcgcg
     4201 cttcttccgt gcaccgaacg gcgaagccgg cagcggcggc catcatgccg gcctggggct
     4261 cgcgctggcg cgggcgctgg cgcgcgccca cgggctgcgg ctggacttcg cgctgcgcga
     4321 tggcgaactc gtcatccgca tcgatggctt cgtgccactg ccctgaaccg ggtgcgctta
     4381 agcaaatcct aagcctgcct tgcgatcatg gcgcgatccc gagaggagcg ccttcgccat
     4441 gccgtccccg tcacgcacgt ttctttgcgc cgccctgctg gccgccacgc tcaccacagc
     4501 tgccgaggcc gcgcccgctg cgctcgcgat cagccccgcg caggcccagg cgatgggcct
     4561 ggagttcgcg ccggcggtcg cggccgactg ggtgcgcgtc gcgcgactgc cggcgcgcgt
     4621 ggaactcgac gccagcgtgc gccggctggt cgcggcgcgg catccgggca cggtggccgg
     4681 cgtgtacgta cgcgaaggcg aggacgtgct cgcggggcaa ttgttgctga gcctgcagag
     4741 ccccgaatgg gccgacgccg tcgcgctggc gcaggcgaac accgcgcggc tgacgcaggc
     4801 cggacaagcc gagacgcgcg cccgcgcgct gctcgaggcc ggcgtcattg cgcggcgcga
     4861 actcgagtcg gcgcaggccg aactggcagc actgcgcgcc accgtggctg gcgacgccgc
     4921 tcgaacagcc ggtgcccgca tcggacgcga cggctcggtg gaattgctgg cgccgtcggg
     4981 gggacgggtg ctgcgccggc cgcacgatcc cggccatgcc gt
//
LOCUS       DAORUQ010000213         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20708_length_4998_cov_5.751568, whole genome shotgun sequence.
ACCESSION   DAORUQ010000213 DAORUQ010000000
VERSION     DAORUQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20708_length_4998_cov_5.751568"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..690)
                     /locus_tag="PLW13_14135"
     CDS             complement(4..690)
                     /locus_tag="PLW13_14135"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQY71571.1"
                     /translation="MRRFTTPTTATLRHALSPLLAVLLALPGTAVQASGKQNSFGNVA
                     EEAPRYRVYTYRQQNGVVAFSDKAPARRSYSVMEFSCFACNPRSTVNWGSTRLFTQEF
                     ATPIDVAARAHGVDPALVRAVIHAESGFNPNARSRKGAIGLMQLMPGTASDMGVSDPT
                     AVQQNIQGGVKYLAMLLAQYKGNVTLATAAYNAGPGAVDKYRGVPPYEETQTYVRRVK
                     LLHDRYRGQS"
     gene            complement(834..>1744)
                     /locus_tag="PLW13_14140"
     CDS             complement(834..>1744)
                     /locus_tag="PLW13_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY71572.1"
                     /translation="RLLTGSVGRLPAVAPPIVLHRDDTLLLTCSAEPGCDAVRDAGGK
                     VEAPARIHCTLDAAFLQVRPGEPVWFDDGRIGGVVEANDGGLITVRITHAGAEGSRLR
                     AEKGINFPETQFALSALTDKDIADLEAVVGFADIVALSFVRSAADVTCLEDHLHRLGA
                     GQLGIVLKIENRQAFENLPGVLLAGLRSPPVGVMVARGDLAVELGFERLSEVQEEILW
                     LCEAAHVPLIWATQMLEGLAKKGVPSRAEVTDAAASARAECAMLNKGPYIVETTRFLG
                     DILGRMHSHQAKRRPTLRSLAVSQKV"
     assembly_gap    1745..1844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1845..2422)
                     /locus_tag="PLW13_14145"
     CDS             complement(<1845..2422)
                     /locus_tag="PLW13_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY71573.1"
                     /translation="MKPSVPQPDASEPELPDALNARLLQRLLSALGEIRAAALQLEAT
                     HAAAIEAIEPAHRASARNLLHYLGVRRHDIRALQADLVGCGLSSLSNMESSTLASIDS
                     VLANLARLTGSEVAPHPPGPVDLRTGALLLADHARALLGAPPQARATRIMVTMPSEAA
                     HDPRLVRELLEAGMDVMRINCAHDDAASWKAMA"
     gene            complement(2502..4889)
                     /locus_tag="PLW13_14150"
     CDS             complement(2502..4889)
                     /locus_tag="PLW13_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HQY71574.1"
                     /translation="MNEIRNDPAAALDADTAARIDAFWRACNYLAAGMIYLRDNPLLE
                     EPLRPEHIKQRLLGHWGASPALSFTWVHLNRLIVKYDLDAIFLAGPGHGAPGVLAPVY
                     LEGTYAEVYPECGEDREGMREFFRSFSFPGGIGSHCTPEMPGSIHEGGELGYSLSHAF
                     GAAFDNPELLVTVVVGDGEAETGPLATAWHGNKFLNPVRDGAVLPVLNLNGYKINNPT
                     VLARVSHEELEALLRGYGWTPRFVEGDDPATMHQAMAAAMEASMLEIRRIQAAARAGG
                     VATRARWPMIVLRSPKGWTGPKTVDGHRIEGTWRAHQVPLAAVRENPAHLRQLEAWLR
                     SYRPGELFDADGRLVAPLRELAPRGTRRMGANPHANGGLLRRALRLPDFRDYAIAVPE
                     PGRASAGNTRALGCLLRDILRANPANFRVFGPDETSSNKLDAVYEASKKTWLADYLPE
                     DADGGELAADGRVMEYLSEHTLEGWLEGYLLSGRHGFLSTYEAFAHVIDSMFNQHAKW
                     LATAAGIPWRAPVSSLNLLITSTVWRQDHNGFTHQDPGFLDVVVTKSPAVTRIYLPPD
                     ANSLLAISDRCLRSTDRVNVIVCDKQVHLQYLDMEAAIRHCAKGIGIWDWASNDQGGE
                     PDVVLAGCGDTATREALAAVALLRGHFPALRIRFVNVVDLYTLTPETEHPHGLPASEF
                     DSLFTVDKPVIFNFHGYPWLIHRLAYRRSNHANLHVRGYKERGNINTPLELAIANEVD
                     RFSLAIDVINRVPELRVAGAHAKQALRDQQFDCRAYAHEHGIDPPEIRDWSWPAP"
BASE COUNT          666 a         1657 c         1793 g          782 t
ORIGIN      
        1 cgctcagctc tgcccgcgat aacggtcgtg cagcagcttg acgcgcctga cgtaggtctg
       61 cgtttcctcg tacggcggca cgccgcggta cttgtcgacc gcgccggggc cggcgttgta
      121 ggccgcggtc gccagcgtca cgttgccctt gtattgcgcg agcagcatgg ccaggtactt
      181 cacgccgccc tggatgttct gctgcaccgc ggtggggtcg ctgacgccca tgtcgctggc
      241 ggttccgggc atcagttgca tcaggccgat cgcgcccttg cgcgagcgcg cgttggggtt
      301 gaagccggac tcggcgtgga tcaccgcgcg caccagcgcc gggtccacgc catgcgccct
      361 ggcggcgaca tcgatggggg tcgcgaattc ctgcgtgaac agccgcgttg aaccccagtt
      421 caccgtcgag cgcgggttgc aggcgaaaca ggagaattcc atcacgctgt acgagcgccg
      481 cgccggggcc ttgtcgctga acgccaccac gccgttctgc tggcggtagg tgtagacgcg
      541 gtaacgcggg gcttcctcgg cgacgttgcc gaagctgttc tgcttgcccg aggcctggac
      601 cgcggtgccc ggcagcgcca gcagcacggc cagcagcggc gacagcgcgt gtcgcagcgt
      661 ggcggtagtc ggggtggtga agcggcgcat cggcacggtc gagctggtgc attcccggtg
      721 gtcgtcgcca aagcgtagcc caggattcgc ggttccggca ggggtgggac cccggaaacg
      781 cgaactgcgg caagcttcgg cgaggggcgc agggccgatg ccgacgcggc cgctcagact
      841 ttctgcgata cggccagcga gcgcagcgtg gggcggcgct tggcctggtg cgagtgcatg
      901 cggcccagga tgtcgccgag aaagcgcgtg gtctcgacga tgtagggccc cttgttcagc
      961 atcgcgcatt cggcgcgcgc gctggcggcg gcgtcggtca cctcggcgcg cgagggcacg
     1021 cctttcttcg cgagtccttc gagcatctgc gtcgcccaga tcagcgggac gtgcgcggcc
     1081 tcgcacagcc acaggatctc ctcctgcact tccgagaggc gctcgaaccc cagctccacc
     1141 gcgagatcgc cgcgcgcgac catgacgccg accggcggcg agcgcaggcc tgccagcagc
     1201 acgcccggca ggttctcgaa ggcctggcgg ttctcgatct tcagcacgat cccgagctgc
     1261 ccggccccga ggcggtgcag gtggtcttcc aggcaggtca cgtcggcggc gctgcgcacg
     1321 aaggacagcg ccacgatgtc cgcgaagccg accaccgctt ccaggtcggc gatgtccttg
     1381 tccgtcagcg cggagagcgc gaattgcgtc tccgggaaat tgatgccctt ctcggcacgc
     1441 agccgcgagc cctcggcgcc ggcgtgcgtg atgcgcaccg tgatcagccc gccatcgttg
     1501 gcctcgacca cgccgccgat ccgcccgtcg tcgaaccaca ccggctcgcc cgggcgtacc
     1561 tgcaggaagg cggcatcgag ggtgcagtgg atccgcgccg gtgcctcgac cttgcccccg
     1621 gcgtcgcgca ccgcgtcgca tccgggctcg gcgctgcagg tcagcagcag cgtgtcgtcg
     1681 cgatgcagca cgatcggcgg cgccaccgcg ggcagcctgc cgacgctgcc cgtgagcagg
     1741 cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccatc gccttccagc
     1861 tcgcggcgtc gtcgtgcgcg cagttgatgc gcatcacgtc catgccggcc tccagcagct
     1921 cgcgcaccag ccgcggatcg tgcgccgcct cgctcggcat ggtcaccatg atgcgcgtgg
     1981 cgcgggcctg gggcggggcg cccagcaggg cgcgcgcgtg gtcggcgagc agcagcgccc
     2041 cggtgcgcag gtcaaccggc cccggcggat gtggcgccac ttcgctgccg gtcagccgcg
     2101 ccaggtttgc cagcaccgag tcgatgctgg cgagcgtgct ggactccatg ttcgacagcg
     2161 acgacagtcc gcagccgacc aggtcggcct gcagggcgcg gatgtcgtgg cggcgcacgc
     2221 ccaggtagtg caggagattg cgcgcgctgg cgcggtgggc cggttcgatg gcctcgatgg
     2281 ccgcggcatg cgtggcttcc agctgcaggg ccgcggcgcg gatctcgccc agcgcgctca
     2341 gcaggcgctg cagcaggcgc gcgttgaggg cgtcggggag ctccggttcg gacgcatccg
     2401 gctgtggaac actgggtttc atcgcggcag gtatcgcatt cacgcaaccc agcatgcgcc
     2461 gggagtatga caaccgtgtt gcagcgcggg gcgggttcgg ctcagggcgc gggccagctc
     2521 cagtcgcgga tctccggcgg gtcgatgccg tgctcgtggg cataggcgcg gcagtcgaac
     2581 tgctggtcgc gcagcgcctg cttcgcgtgc gcgccggcca cgcgcaactc cggcacgcgg
     2641 ttgatcacgt cgatcgcgag gctgaagcgg tcgacctcgt tggcgatcgc cagttccagc
     2701 ggcgtgttga tgttgccgcg ctccttgtaa ccgcgcacgt gcaggttggc gtggttgctg
     2761 cgccggtagg cgaggcggtg gatcagccag gggtagccgt ggaaattgaa gatgaccggc
     2821 ttgtcgaccg tgaacaggct gtcgaactcg ctcgccggca ggccgtgcgg gtgctcggtt
     2881 tccggcgtca gcgtgtacag gtccacgacg ttgacgaagc ggatccgcag cgccggaaaa
     2941 tgtccgcgca acagcgccac cgccgccagc gcctcgcgcg tggcggtgtc gccgcagccg
     3001 gccagcacca cgtcgggttc gccaccctgg tcgttgctgg cccagtccca gatcccgatg
     3061 cccttggcgc agtggcggat cgccgcctcc atgtcgaggt actgcaggtg cacctgcttg
     3121 tcgcagacga tcacgttcac gcggtcggtg ctgcgcaggc agcggtcgct gatcgcgagc
     3181 agcgagttgg cgtccggcgg caggtagatg cgcgtgaccg cggggctctt ggtgacgacc
     3241 acgtcgagga aacccgggtc ctggtgcgtg aagccgttgt ggtcctggcg ccataccgtg
     3301 gaggtgatca gcaggttcag cgaggacacg ggggcgcgcc acgggatccc ggcggccgtc
     3361 gccagccact tcgcgtgctg gttgaacatg gagtcgatca cgtgcgcgaa ggcttcgtag
     3421 gtcgacagga agccgtggcg gccgctgagc aggtagcctt ccagccagcc ctcgagcgtg
     3481 tgctcgctca ggtactccat cacccgcccg tcggcggcga gctcgccgcc gtcggcgtcc
     3541 tcgggcagat aatccgcgag ccaggtcttc ttgctggcct cgtagacggc atcgagcttg
     3601 ttcgagctgg tctcgtcggg gccgaacacg cggaagttgg cggggttggc gcgcaggatg
     3661 tcgcgcagca ggcagcccag cgcgcgcgtg ttgccggcgc tggcgcgtcc cggctcgggc
     3721 accgcgatcg cgtaatcgcg gaaatccggc agccgcagcg cgcggcgcag caggccgccg
     3781 ttggcgtgcg ggttggcgcc catgcgccgc gtgccgcgcg gcgccagttc gcgcagcggc
     3841 gccaccagtc gcccgtcggc gtcgaacagt tccccgggcc ggtacgaacg cagccaggcc
     3901 tcgagctggc gcaggtgagc ggggttctcg cgcaccgccg ccagcggcac ctggtgggcg
     3961 cgccaggtgc cctcgatgcg gtggccgtcc acggtcttcg gcccggtcca gcccttgggg
     4021 ctgcgcagca cgatcattgg ccagcgcgcc cgcgtggcca cgccgccggc gcgtgccgcg
     4081 gcctggatgc ggcggatctc cagcatgctc gcttccatcg ccgccgccat cgcctggtgc
     4141 atggtggcgg gatcgtcgcc ctcgacgaaa cgcggcgtcc agccatagcc gcgcagcagc
     4201 gcctcgagtt cctcgtggct gacgcgcgcg agcacggtgg gattgttgat cttgtagccg
     4261 ttgaggttca gcaccggcag caccgcgccg tcgcgcaccg ggttgaggaa cttgttgccg
     4321 tgccaggcgg tggcgagtgg gccggtctcg gcctcgccgt cgcccaccac caccgtgacc
     4381 agcagctccg gattgtcgaa ggccgcgccg aaggcgtgcg agagcgaata gccgagctcg
     4441 ccgccctcgt ggatggagcc cggcatttcg ggcgtgcagt ggctgccgat gccaccgggg
     4501 aaggagaagc tgcggaagaa ctcgcgcatg ccctcgcggt cctcgccgca ctcggggtac
     4561 acctccgcgt aggtcccctc caggtacacc ggcgccagca cgccgggcgc gccgtgtccg
     4621 gggccggcga ggaagatcgc gtccaggtcg tacttcacga tcaggcggtt caggtgcacc
     4681 caggtgaagc tgagcgcggg gctcgcgccc cagtgcccga gcaggcgctg cttgatgtgc
     4741 tccgggcgca gcggctcttc cagcagcggg ttgtcgcgca ggtagatcat gccggcggca
     4801 agataattac aggcgcgcca gaaggcatcg atgcgtgcgg cggtgtcggc atcgagcgcg
     4861 gcggccggat cgttgcgtat ctcgttcatg gatcgtgtct cctgcgatgc gtggcgcgcg
     4921 ccggcgcgtg tcagggtgtg gctcggggtt cgagcagctc gatgacctcg cgcgcgatct
     4981 cgagctcctc gttggtcg
//
LOCUS       DAORUQ010000214         4978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20830_length_4978_cov_6.193581, whole genome shotgun sequence.
ACCESSION   DAORUQ010000214 DAORUQ010000000
VERSION     DAORUQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20830_length_4978_cov_6.193581"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1497
                     /locus_tag="PLW13_14155"
     CDS             289..1497
                     /locus_tag="PLW13_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71575.1"
                     /translation="MAAKSGRWHAGMKKSPLAVIFLTVFLDLVGFGIIIPLSPYLARE
                     FGASAFEVGMLMAVYSAMQFLFSPFWGGLSDRLGRRPILLVSIAGTALAHTLFYFAHD
                     LWLLFLARTLAGIFGANISTAMAYIADVTSEKDRSKNMGLIGAAFGLGFVLGPAIAGM
                     LTGYGENFPALAAAFLSLANFVMAYFVLGESLSAENRRPRAKKSRIRNIVEKVQRPVI
                     GSLLFAQFATGLAMANMEASLFLYMQDRFGWGVRESSYGFAYVGVCIAFTQGYLVRKY
                     LPIHGERGMLVAGFLLFILGLLFTGLSPWIWLLAIAMTALAVGNGFINPAVSGSISLL
                     TPAHEQGEVLGVNQSLAALARIFGPLMGGYAYMHVTHTAPFFMAAGLGAVGLAFIWLA
                     RARIPQAAKA"
     gene            1640..2131
                     /gene="ssb"
                     /locus_tag="PLW13_14160"
     CDS             1640..2131
                     /gene="ssb"
                     /locus_tag="PLW13_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQY71576.1"
                     /translation="MAKGINKVILIGNMGAEPETRYLPSGGAVTNIRLATSESWKDKN
                     TGQQQERTEWHRVVFFNRLAEIAGEYLHKGSKVYIEGSLRTRKWQDKEGKDQYTTEIV
                     ANEMQMLDSRGGGGGGMSGGDDYGSGGGFAPEPRQPPARAPASAPAGKSDEPPAWDDD
                     IPF"
     gene            2171..3019
                     /locus_tag="PLW13_14165"
     CDS             2171..3019
                     /locus_tag="PLW13_14165"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQY71577.1"
                     /translation="MKTVIIGADCRLGLELAANLQGRDIPFVSISSRDPVLDSVKLLL
                     HAFAYHGATQVVNALSHELFSGDDPAAYKRSLLVTKNLCKACRAHEAALIHLSDDSMY
                     AGRKSGAYREKDKPDSADPRVARVLKAESYVNKRAPRHIVLRCGPLFAPAGHNLFTML
                     AAQLEKGHLLECTEDKLCPTPANDIARVVVAIILQLDCAAEPWGTYNYCSSDASSLYN
                     FAEAVIALASQYGRIRRDEVRLQAVPGKDRNVILNCHQILNTFGIKQRPWRAALPAVV
                     AEYCRS"
     gene            3266..3787
                     /gene="moaB"
                     /locus_tag="PLW13_14170"
     CDS             3266..3787
                     /gene="moaB"
                     /locus_tag="PLW13_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HQY71578.1"
                     /translation="MSASADKKPLNIAVLTVSDTRTEATDTSGQYLVESLRAAGHTLA
                     EKAIVIDDIYQLRAIVSRWIADPDVHVILGTGGTGFSGRDSTPEALMPLFDKVIEGFG
                     ETFRALSVAEIGSSSLQSRAFGGLANRTVIFCMPGSTGACRTAWEGIIRDQLDSTFRP
                     CNFAPIVLGSYAH"
     gene            3798..>4978
                     /locus_tag="PLW13_14175"
     CDS             3798..>4978
                     /locus_tag="PLW13_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQY71579.1"
                     /translation="MTPQGLTSVDAALAHILADVTPLEGTESLPLARLRGRVLAADAI
                     ATIDVPPADNSSMDGYALRHADLEAGQRDFAVSQRVAAGTVGTMLMPGTVARIFTGAE
                     VPPGADCVVIQENCSVADGVVTVLQPVAPEDNIRRRGQDIAAGGAIVAAGTRLGGAHA
                     GLLAAVGMARATVFARLRVAILSTGDELVEPGETAGPGQIYNSNRYLLTGMLEDLGCE
                     VIDGGLVADTPEATRACLRQAAEQADVVITTGGVSVGDEDHVKAAVEELGTLGLWKLA
                     IKPGKPLAYGRILGKPFFGLPGNPSSVFVTFHVVTRPYLQRMQGIAGDVMARELVVAA
                     DFDRPRPDKRQEYLRARLVNGGDGARVALYPNQSSGVLSSVAWADCLVVVPPGTTVAR
                     G"
BASE COUNT          788 a         1715 c         1675 g          800 t
ORIGIN      
        1 gtcaggtccc ttgacgcgat cgagtatgcc acgcgctcgc cggggcgatc cagacgctgt
       61 cggcgccgca cggacgcgcg acccggcggt atttcgtacg atggtacgag cggagcatag
      121 ttgctaggat tccgcgcgcc gcgtcgcgcc tgtcggctac ccgggttttc atcatggcaa
      181 cggatatttc aggccagggt ctccgggcct gaacggtgag ggcatggctg aaattgccgc
      241 ccgctcacgc gacatcggca gcggaccggc gggagatatc gcgatgaaat ggcagcaaag
      301 tcagggcgtt ggcacgccgg catgaagaaa agccccctgg cggtgatctt tctcaccgtt
      361 ttcctcgatc tcgtcggctt cggcatcatc attccgctca gcccctacct ggcgcgcgaa
      421 ttcggggcct ccgccttcga ggtcgggatg ctcatggccg tctattcggc gatgcagttc
      481 ctgttctcgc cgttctgggg cgggctcagc gaccgcctgg gccgccgccc catcctgctc
      541 gtgagcattg ccggcacggc gctggcccac acgctgttct atttcgcgca cgatctctgg
      601 ctgctgttcc tggcgcgcac cctggccggc atcttcggcg ccaatatttc cacggcgatg
      661 gcctatatcg ccgacgtcac ctcggaaaag gatcgctcca agaacatggg gctgatcggc
      721 gcggcgttcg ggctcggctt cgtgctgggg cccgcgattg ccgggatgct gaccggctat
      781 ggcgagaact ttcccgcgct ggcggcggca ttcctcagcc tggccaactt cgtcatggcg
      841 tatttcgtgc tcggggaatc gctgagtgcc gaaaaccgcc ggccgcgcgc gaaaaaatcg
      901 cggatcagga atatcgtgga gaaagtgcag cgcccggtga tcggctcgct gctgttcgcg
      961 cagttcgcca ccgggctggc gatggcgaac atggaagcgt cgctcttcct ctacatgcag
     1021 gaccgcttcg gctggggcgt gcgggaatcg agctacggct ttgcctatgt cggcgtgtgc
     1081 atcgcgttca cccagggata cctggtcagg aagtacctgc cgatccacgg cgaacggggc
     1141 atgctggtcg ccggcttcct gctgttcatc ctgggcttgc tgttcacggg cctcagcccg
     1201 tggatctggc tgctcgccat cgccatgacc gcactggcgg tcggcaacgg gttcatcaac
     1261 ccggccgtct cgggcagcat cagcctgctg acgccggcgc acgaacaggg cgaagtgctc
     1321 ggcgtgaacc agtcgctcgc cgcgctggcg cggattttcg ggccactcat ggggggctat
     1381 gcctacatgc acgtgacgca cacggccccg tttttcatgg cggcgggcct gggcgccgtg
     1441 ggcctggcgt tcatctggct ggcgcgcgcc cggatcccgc aggcggccaa ggcctagctg
     1501 cccgcgcggc agacccggga gccgcgcgac actccacacg gatgctgtgc cctgcggcca
     1561 gcctcattca ttgacaccgg ccggcggcgc atgttgtgat ccgcggccca ggacgagcca
     1621 cccgctcaag gaggaaatca tggccaaggg catcaacaag gtgatcctga tcggcaatat
     1681 gggtgctgaa cccgaaacgc gttacctgcc ctcgggcggc gcggtcacca acatccgtct
     1741 cgcgacctcg gaatcgtgga aggacaagaa caccggccag cagcaggagc gcaccgagtg
     1801 gcaccgggtc gtgttcttca accgcctggc ggagatcgcc ggcgagtacc tgcacaaggg
     1861 cagcaaggtc tacatcgaag gttcgctgcg cacccgcaag tggcaggaca aggaaggcaa
     1921 ggaccagtac accaccgaga tcgtcgccaa cgagatgcag atgctcgaca gccgcggcgg
     1981 tggcggcggc ggcatgtccg gcggtgacga ctacggttcc ggcggcggtt tcgcccccga
     2041 gccgcgccag cctcccgcgc gcgcacccgc gtcggcaccg gcgggcaagt ccgacgagcc
     2101 cccggcctgg gatgacgaca tcccgttctg atccgcgtcg acgcatcggc agtcgggcag
     2161 ggatgccggc atgaaaaccg tcatcatcgg cgccgactgc cgtctcgggc tcgagctcgc
     2221 cgccaacctg caggggcggg acatcccgtt cgtctccatt tccagccgcg atcccgtgct
     2281 cgacagcgtg aagctgctgc tgcacgcctt cgcctaccac ggcgcgacgc aggtggtgaa
     2341 cgcgctgtcg cacgagctgt tcagcggcga cgatcccgcg gcctacaagc gcagcctgct
     2401 ggtgacgaag aacctgtgca aggcctgccg cgcccatgag gcggcgctga tccacctctc
     2461 cgacgacagc atgtacgccg ggcgcaagag tggcgcctac cgcgagaagg acaagcccga
     2521 cagcgccgat ccgcgcgtcg cgcgcgtgct gaaggccgag agctacgtga acaagcgcgc
     2581 gcccaggcac atcgtgttgc gctgcggtcc gctgttcgcg cccgccggtc acaacctgtt
     2641 cacgatgctc gccgcgcagc tcgagaaggg gcacctgctc gaatgcaccg aggacaagct
     2701 ctgccccacg ccggccaacg acatcgcgcg cgtggtggtg gcgatcatcc tgcagctcga
     2761 ttgcgccgcc gagccctggg gcacctacaa ctactgcagc tcggatgcgt ccagcctgta
     2821 caacttcgcc gaggcggtga tcgcgctggc ctcgcaatac ggccgcatcc gccgcgacga
     2881 ggtgcggctg caggcggtgc cgggcaagga tcgcaacgtg atcctcaact gccaccagat
     2941 cctcaacacc ttcggtatca agcagcgccc ctggcgtgca gccttgccgg cggtggtggc
     3001 ggaatattgt cgtagttaag catcaggtcg cgggcatggc ccggtcgtgc ggggggacgt
     3061 catcgaatcg cgggcatggc ccgctcctac ggggaacggc tgggtcgcgg gcatggcccg
     3121 gtcgtgcggg ggaacgtcat tggtcgcggg catggcccgg tcccgcggta ggagcgggcc
     3181 atgcccgcga ccgaaaaaat catcgtgttg acgccggtgc gcccgccacg ccaccattca
     3241 caccacacgc aaggagtacc accgcatgag cgccagcgcc gacaagaagc ccctgaacat
     3301 cgccgtcctc accgtctcgg acacccgcac cgaggccacc gacacctcgg gccagtacct
     3361 cgtcgagagc ctgcgtgccg ccggtcacac gctggcggaa aaggccatcg tcatcgatga
     3421 catctaccaa ctgcgcgcga tcgtctcgcg ctggatcgcc gaccccgacg tgcacgtgat
     3481 cctcggtacc ggcggcaccg gcttcagtgg ccgcgattcc acgcccgagg cgctgatgcc
     3541 gctgttcgac aaggtgatcg agggcttcgg cgagaccttc cgcgccctct cggtggcgga
     3601 gatcggcagc tcctcgctgc agtcgcgcgc cttcggcggc ctggccaacc gcaccgtgat
     3661 cttctgcatg cccggttcca ccggcgcctg ccgcacggcg tgggaaggca tcatccgcga
     3721 ccagctcgac agcacgttcc ggccctgcaa tttcgcgccc atcgtgctcg gcagctacgc
     3781 gcactgagcc gccggccatg acgccgcagg gactgacttc cgtcgacgcc gcgctcgcgc
     3841 acatccttgc cgacgtcacc ccgctcgagg gcacggaatc gctgccgctc gcccggctgc
     3901 gcgggcgcgt gctcgccgcc gacgcgatcg ccacgatcga cgtgccgccc gccgacaaca
     3961 gctccatgga cggctatgcg ctgcgccacg ccgacctcga agccgggcag cgcgacttcg
     4021 cggtcagcca gcgcgtcgcc gccggcacgg tcgggacaat gctcatgccc ggcactgtgg
     4081 cgcgcatctt caccggcgcc gaagtgccgc ccggtgccga ttgcgtcgtg atccaggaaa
     4141 actgcagcgt ggccgacggc gtggtcacgg tcctgcagcc cgtggcgccg gaagacaaca
     4201 tccgccggcg cggccaggac atcgccgccg gcggcgcgat cgttgccgcg ggcacgcgcc
     4261 tcggtggggc gcacgcgggc ctgctggccg cggtcggcat ggcgcgggcc acggtgttcg
     4321 cgcgcctgcg cgtggcgatc ctctcgaccg gcgacgagct ggtcgaaccg ggcgagacgg
     4381 cggggccggg gcagatctac aactccaacc gctacctgct caccggcatg ctagaggatc
     4441 tgggttgcga ggtgatcgac ggcggcctgg tggcggacac gcccgaggcc acccgcgcct
     4501 gcctgcggca ggccgcggag caggccgacg tggtcatcac caccggcggc gtctcggtgg
     4561 gcgacgagga ccacgtgaag gccgcggtcg aggaactggg cacactgggg ctgtggaagc
     4621 tcgcgatcaa acccggcaag ccgctggcct acgggcgcat cctcggcaag ccttttttcg
     4681 gtttgcccgg caatccctcg tcggtgttcg tgaccttcca cgtggtgacg cggccctatc
     4741 tgcagcgcat gcagggtatc gccggcgacg tgatggcgcg cgagctggtg gtcgcggcgg
     4801 atttcgatcg cccgcgtccc gacaagcgcc aggaatacct gcgcgcgcgg ctcgtgaacg
     4861 gcggcgacgg cgcgcgcgtg gcgctgtacc cgaaccagag ttccggtgtg ctgtcgtcgg
     4921 tggcgtgggc cgattgcctg gtggtggtgc cgccgggcac gaccgtggcg cgcggcga
//
LOCUS       DAORUQ010000215         4959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_20967_length_4959_cov_6.303834, whole genome shotgun sequence.
ACCESSION   DAORUQ010000215 DAORUQ010000000
VERSION     DAORUQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20967_length_4959_cov_6.303834"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLW13_14180"
     CDS             complement(<1..1100)
                     /locus_tag="PLW13_14180"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HQY71580.1"
                     /translation="MSSRIARQGFLCLALGLLVLAARAETVKGLYEADVPVASQDEQA
                     RAVAVDTGFRQVLVKVAGSAGVLDNPGVAAALPRANNYLLQFYFTQIDYPPRDSGPAR
                     EPGLHVVFAPRAVDDILRRAGEPMLPPNRPATLLWLTLDDGAGPRLLSSAVDTEVADW
                     LSFHAARRAMPLLFPQMDIEDSAALTPEQVAALDDAATLRASARYGAESVLAARLVRA
                     SDGSWLGEWRYLAGAQGSTGQPGDAAVGDAAPGDVVAGDVAAGAVPGNNPTSSDGSGS
                     APTLDGLAGQLVDFMADAVAARYAVQTGIANTEQLRLRVDGVAGFAAYEKLSRLLKAM
                     TSVRGLQLVLIDRDTLFFDIVTDSNFASVARE"
     gene            1235..2284
                     /gene="purM"
                     /locus_tag="PLW13_14185"
     CDS             1235..2284
                     /gene="purM"
                     /locus_tag="PLW13_14185"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQY71581.1"
                     /translation="MSEASNDGLSYKDAGVDIDAGNALVANIKGVARRTRRPEVLGGL
                     GGFGALCEIPKRYKEPVLVSGTDGVGTKLRLAMQLGIHDSIGIDLVAMCVNDLVVAGA
                     EPLFFLDYYATGRLNVDVATRVIQGIGTGCEQAGCALVGGETAEMPGMYEGEDYDLAG
                     FCVGVVEKSRIIDGSRVAPGDALIALASSGAHSNGYSLIRKILELSGAALDADLDGRP
                     LGQALMAPTRIYVKPLLALIEQCQVNALAHITGGGLLENIPRVLPAGSCAVLDAASWQ
                     EPELFRWLARTGKVARMEMYRTFNCGVGMVVCVPEAGCEAAIAQLRAAGENAWRIGHI
                     ETAPEGSEQVRMLGC"
     gene            2274..>2907
                     /gene="purN"
                     /locus_tag="PLW13_14190"
     CDS             2274..>2907
                     /gene="purN"
                     /locus_tag="PLW13_14190"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQY71582.1"
                     /translation="MAAEAHPSTQPCRLVVLVSGSGSNLQSFIDACRDPSYPCTVAAV
                     ISNKAGVRALERARLAGIAAEVLDHQGFANRAAFDAELARRIDAHAPDLVILAGFMRI
                     LTPGFVRHYAGRLLNIHPSLLPLYPGLHTHQRALAAGDAEHGATVHFVTEDLDGGPAI
                     VQARVPVLPGDTPETLAARVLEREHLIYPLAARLYAEGRLCLRGDQATLDG"
     assembly_gap    2908..3007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3008..3535
                     /locus_tag="PLW13_14195"
     CDS             <3008..3535
                     /locus_tag="PLW13_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY71583.1"
                     /translation="VDAARELFHRRGYGETSLADIARESGVPVGNVYYYFKTKEDIAA
                     AVIEQHRAHMSGIAQLAETRPTARERILSMLDSLACGCDSIAHSGCPMGSLCVEFGKR
                     DTALREEANRLLLRMIDWVERQFAGMGRSDARALAIQLVSTVQGASVLSLAMHDPAIV
                     RQELARMKTVVEALG"
     gene            3738..4136
                     /locus_tag="PLW13_14200"
     CDS             3738..4136
                     /locus_tag="PLW13_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71584.1"
                     /translation="MKTATNNRTGSRTTTRRGALALLLATTLGIGGHAANAAQAPSAA
                     ELEHCLQRAGLELKLHERDLRLLSAQKFALGRERHMIALNTEVALGNASMNPRLYCTV
                     ERGGEISLVQSMPRLPHRPAATLVADHGHP"
     gene            complement(4368..4934)
                     /gene="dcd"
                     /locus_tag="PLW13_14205"
     CDS             complement(4368..4934)
                     /gene="dcd"
                     /locus_tag="PLW13_14205"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQY71585.1"
                     /translation="MSIKSDRWIRRMAAEHGMISPFEPDQVRHTAAGKVISFGTSSYG
                     YDVRCSDEFKIFTNVHSATVDPKNFDPRSFVDIKSDSCIIPPNSFALARTVEYFRIPR
                     SVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTNLPAKIYANEGVAQMLFF
                     ESDEVCETSYRDRGGKYQGQTGVTLPRA"
BASE COUNT          775 a         1700 c         1665 g          719 t
ORIGIN      
        1 atctcgcgcg ccaccgacgc gaagttgctg tcggtgacga tgtcgaagaa cagggtatcg
       61 cgatcgatga gcaccagctg caggccgcgc accgaggtca ttgccttcag cagccgcgag
      121 agtttctcgt aggccgcgaa gccagccacg ccatcgacgc gcaagcgcaa ctgctcggtg
      181 ttcgcgatcc cggtctgcac cgcgtagcgc gcggccaccg catcggccat gaaatccacg
      241 agttgcccgg cgagcccgtc cagcgtgggc gcgctgcccg acccgtcgct gctcgtcggg
      301 ttgttgcccg gcacggcacc agcggcaacg tcgccggcca ccacatcgcc cggcgcagcg
      361 tcacccaccg cagcgtcgcc cggctgccct gtgctgccct gcgcgccggc caggtaacgc
      421 cattcgccga gccaggagcc gtcgctggcg cgcaccaggc gcgcggccag caccgactcg
      481 gcgccgtagc gcgccgaagc gcgtagcgtc gccgcatcat ccagcgccgc cacctgctcc
      541 ggggtgagcg cggcgctgtc ctcgatgtcc atctgcggga acagcaacgg catggcgcgg
      601 cgcgcggcat ggaagctcag ccagtcggcg acctcggtgt cgacggcact gctcagcagg
      661 cgcggccccg cgccgtcatc cagcgtgagc cagagcaatg tcgccgggcg gttgggcggc
      721 agcatcggct cgccggcacg gcgcaggatg tcgtccaccg cccgcggcgc gaacaccacg
      781 tggagccccg gctcccgggc gggcccgctg tctcgcggcg gatagtcgat ctgcgtgaag
      841 tagaactgca gcaggtagtt gttggcgcgc ggcagcgccg cggcaacgcc ggggttgtcg
      901 agcacgcctg ccgatcccgc gaccttgacc agcacctgcc ggaagccggt gtcgaccgcg
      961 accgcgcgcg cctgctcgtc ctggctcgcg accggcacgt cggcctcgta gaggcccttg
     1021 accgtttccg cccgggccgc cagcacgagc aggccgaggg ccagacagag aaagccctgg
     1081 cgagcgattc gggaactcat gcgtttgcgg ctgccgcgat ccgatggtga gcggcattct
     1141 agcaaggccg gcggcttcac acagcgccgg acgcggtttg ctgggccgga gggcctgctg
     1201 ctagaatcgc gcgccccagc gaaggcgaac gcccatgtcc gaagccagca acgacggtct
     1261 ctcctacaag gatgcggggg tcgatatcga cgccggcaat gccctggtcg cgaacatcaa
     1321 gggcgtggcg cgccgcacac ggcgccccga ggtgctgggc gggctcggcg gcttcggcgc
     1381 gctgtgcgag attcccaagc gctacaagga accggtgctg gtctccggca ccgacggtgt
     1441 cggcaccaag ctgcgcctgg cgatgcagct cggcatccac gacagcatcg gcatcgacct
     1501 ggtggcgatg tgcgtcaacg acctcgtggt cgccggcgcc gaaccgctgt tcttcctcga
     1561 ttactacgcc accggccggc tcaacgtcga cgtggcgacc cgggtcatcc agggcatcgg
     1621 caccggctgc gagcaggcag gctgcgcgct ggtcggcggc gagaccgccg agatgcccgg
     1681 catgtacgag ggcgaggatt acgacctcgc cggcttctgc gtcggcgtgg tggagaagag
     1741 ccggatcatc gacggcagcc gcgtggcgcc gggcgatgcg ctgatcgcgc tggcctcgag
     1801 cggcgcgcac tccaacggct attccctgat ccgcaagatc ctcgagctga gcggtgccgc
     1861 gctcgacgcc gatctcgacg gccgcccgct cggccaggcg ctgatggcgc ccacgcgcat
     1921 ctacgtcaag ccgctgctcg cgctgatcga gcagtgccag gtgaacgcgc tcgcgcacat
     1981 caccggcggc ggcctgctcg agaacatccc gcgggtgctg cccgcgggca gctgcgccgt
     2041 gctcgatgcc gccagctggc aggagccgga gctgttccgc tggctcgcgc gcaccggcaa
     2101 ggtggcgcgc atggagatgt accgcacctt caactgcggc gtcggcatgg tggtctgcgt
     2161 gcccgaggcc gggtgcgagg cggcgatcgc gcagcttcgc gccgcgggcg agaacgcctg
     2221 gcgcatcggg cacatcgaga ccgcgcccga gggcagcgaa caggtgcgca tgcttggctg
     2281 ctgaggcgca tccgtccacg cagccctgtc gcctcgtcgt tctggtctcg ggcagcggca
     2341 gcaacctgca gagcttcatc gatgcctgtc gcgacccatc ctacccctgc accgtcgccg
     2401 cggtaatcag caacaaggcc ggcgtgcgcg ccctggagcg tgcgcgcctg gccggcatcg
     2461 ctgccgaggt actggatcac cagggcttcg cgaaccgcgc ggcattcgat gccgagctcg
     2521 cacgtcgcat cgacgcgcac gcccccgacc tcgtgatcct ggccggcttc atgcgcatcc
     2581 tcacgcccgg cttcgtgcgc cactacgccg ggcggctgct gaacatccac ccgtccctgc
     2641 tgccgctcta cccggggttg cacacgcacc agcgcgcgct cgcggcgggc gacgccgagc
     2701 acggcgccac ggtgcacttc gtcaccgagg acctcgatgg cggtccggcg atcgtgcagg
     2761 ccagggtacc ggtgctgccc ggcgacacgc cggaaacgct cgcggcgcgc gtgctcgaac
     2821 gcgagcacct gatctacccg ctggcggcgc gtctctacgc cgaagggcgc ctgtgcctgc
     2881 ggggcgatca ggccacgctc gatggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnngtc gatgccgcgc gcgagctgtt ccatcgccgc ggctacggcg agaccagcct
     3061 ggccgatatc gcgcgcgagt ccggcgtgcc ggtgggcaac gtctattact acttcaagac
     3121 caaggaggat atcgccgcgg ccgtcatcga gcagcaccgc gcccacatgt cgggcatcgc
     3181 gcagctcgcc gagacccggc ccacggcgag ggagcgcatc ctgagcatgc tcgacagcct
     3241 cgcctgcggc tgcgactcga tcgcgcacag cggctgcccg atgggctcgt tgtgcgtgga
     3301 gttcggcaag cgcgacacgg cgctgcgcga ggaagccaac cgcctgctgc tgcgcatgat
     3361 cgactgggtc gaacgccagt tcgccggcat ggggcgcagc gatgcacgcg ccctggcgat
     3421 ccagctggtg tccacggtgc agggcgcgag cgtgctgtcg ctggcgatgc acgatcccgc
     3481 catcgtgcgc caggaactgg cacgcatgaa aaccgtcgtc gaggcactgg gatgatgtct
     3541 tcgcgcaacc cgcgccagac aggcacacat ttttcggaga atttaagttg gttaactcac
     3601 ttactttcgc ggatcgccgc tccgtaggcg gcgccgccaa ccagaccccg cgctgccggc
     3661 agcggtcgct gccggaacgc cgggcacctg ccacgagcag ggttcctgcc accaccccac
     3721 gagggaagga gaacgccatg aaaaccgcaa cgaacaaccg caccggctcg cgcacaacga
     3781 cccgccgcgg cgcgctggcg ctgctgctgg ccaccacgct gggcatcggc ggtcacgccg
     3841 cgaacgcggc gcaggcacct tcggccgccg agctcgagca ctgcctgcag cgggccgggc
     3901 tggaactgaa gctccacgaa cgcgatctgc gactgctgag cgcgcagaag ttcgcgctcg
     3961 ggcgcgagcg ccacatgatc gcgctcaaca cggaggtcgc gctggggaat gcgtcgatga
     4021 acccgcggct ctactgcacg gtggagcgcg gcggcgagat cagcctggtg cagagcatgc
     4081 cgcgcctgcc gcaccggccg gcggcgaccc tggtggccga ccacggccat ccgtgatggc
     4141 ggccgcaacg ccaccacgtc tgcgatgaac cgggggtgcc accacggcaa ccagtgccac
     4201 gctggctttc ttgcgaggac cgtctgtcgc ctggacggcg acagtcgagc ctacagggat
     4261 gtattcacgg cgtgtccgag caggagagcc agcgtggcac tggcggggac gaaccacaag
     4321 tcggggcaaa aacggtccat ttcaggttga agcctgagcg tcgaaactca ggcgcgcggc
     4381 agcgtgaccc cggtctggcc ctggtacttg ccgccacgat cgcggtacga ggtctcgcag
     4441 acctcgtcgc tctcgaagaa cagcatctgc gccacgccct cgttggcgta gatcttggcg
     4501 gggaggttgg tggtgttgga gaactcgagc gtcacgtgcc cttcccattc gggctccagc
     4561 ggcgtgacgt tcacgatgat gccgcagcgc gcgtaggtcg acttgccgag gcagatcgtc
     4621 agcacgctgc gcgggatgcg gaagtactcc acggtgcgcg ccagcgcaaa ggaattcggc
     4681 gggatgatgc acgagtccga cttgatgtcg acgaagcttc gcgggtcgaa gttcttcggg
     4741 tccaccgtcg ccgagtgcac gttggtgaag atcttgaact cgtccgagca gcgcacgtca
     4801 tagccgtagc tcgaggtgcc gaaggaaatg accttgccgg ccgccgtatg ccgcacctgg
     4861 tcgggctcga aggggctgat catgccgtgc tcggccgcca tgcggcgaat ccacctgtcc
     4921 gatttgatcg acatcgctgt gctgcttccc gattgagtc
//
LOCUS       DAORUQ010000216         4923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_21180_length_4923_cov_6.377979, whole genome shotgun sequence.
ACCESSION   DAORUQ010000216 DAORUQ010000000
VERSION     DAORUQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21180_length_4923_cov_6.377979"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLW13_14210"
     CDS             complement(<1..1127)
                     /locus_tag="PLW13_14210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71586.1"
                     /translation="MAIRIRRFAPTVLAMAVAGAAAPWAQAQQDGARATGLEEVIVTA
                     ERREASLQDTPISVLAFGADMLEKLNVDDLGDIQFSVPNLFMRQFPNSQASLRTYMRG
                     VGNNDVGMSTDPSVGVYIDGIYMARSIGLGSEVASLERIEVLRGPQGSLYGRNTIGGA
                     INMITAKPKGEWGFKGGLSAGDRDYVKARAEVDTARVAGLAAKLSLMKSEKDGWIEND
                     GRGPNFGDNDSAAGRLAINWSPSDNFSADYAYDYSDVDSGTIYYQNIGPSQFGFESVP
                     FRSDRQDNVRPRVPFEESNVEVSGHSLTLAWEISDDLTLKSLTGYREVEQDIYQDYGA
                     NPVTTRLFANAPFLNEQDQFSQEFQLLGKLMDGGLEYVLGA"
     gene            complement(1322..3367)
                     /locus_tag="PLW13_14215"
     CDS             complement(1322..3367)
                     /locus_tag="PLW13_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY71587.1"
                     /translation="MQSFQHLASPIRIGAMTVGNRMVMSAMTTNYGSADFEVTERLIR
                     YHEARARGGVGLITVEMCSVDVAQRYQPQSLSLGDDRFIAGHRELVRRVHAHGARIQP
                     QISHPGPESMTDPVGPSVNVNAGTGWPSRVLEAEEMERVIAQYGDAAVRAREAGYDGM
                     ELHAAHGYMLLGSFLCPVRNRREDEYTGATIEGRTRLLVRVIRHIKQRAGADFPITLR
                     ISGNEDAFDGRMLNDTQLIAPLLEQAGIDAFQVSGGVSHDKIVAQIVCGAHYRDGYNT
                     AVAAAVRKVVNVPVMVVGRIHDPVLAEKIIAEGDADMVAMARPLLADPELPAKVLSGR
                     TLEIRRCLSCQNCIDSMLIAPFDANMNCAVNAQSGREAELAITPAAVRKRVLVVGGGP
                     AGMEAARVAALRGHEVRLVERGGRLGGSLFYASIVHADNQRLLDYLLGAMSRLPVRVE
                     LGRALEPAECADADAVVVATGARMVRPVIPGMEAAHVYDATEVRGWFSGTLSAEEAAR
                     WPALLKLLGHHVMPRVQQWLRPGHVRAASRHFLPLGKRVCVIGSDLPAVELADFIAHR
                     GRRVSLIAEDQWFAQDVGPKRRQEQLSALDRLGVEVLGGVRLTGIDAGAVRFEYAGRA
                     GSVPADTVIIAGSPAPDTRLADALAGRVGEIHAIGDCTGPGLLRKAVEDGMRAGTAL"
     gene            3469..4818
                     /locus_tag="PLW13_14220"
     CDS             3469..4818
                     /locus_tag="PLW13_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71588.1"
                     /translation="MSQSSDASRYRPIPFVSGAVPGGGHFEEFCAHPAFFLQRAFREC
                     GEVCEFDQGGMRTVLMVGPEAHEAVFRAPDEQLSAPAAYQYMVPIFGPGVQYGAPIEI
                     ERQQLKMHAKGLRAERLTYYAPIVAREVEDWISGWGDEGEMDFYEAFADLTIKTSTHC
                     LLGAEFRYSLTDEFARHYHDLGEAADAAGTVDHTQQKAVYDRRDRARKALGELIIERI
                     HRRRNAGETHDDLLQVYMDATLLDGSRLSDEQIAGMVVWFMFAGHHTSANTAAWTLVE
                     LARYPDFGREVTAEVDQLFATETELSFRALRKMPLMEGFIREILRAHPPLNALCRRVM
                     QDFHYKDYLIEAGKNVMLSPYVAHRLPQYFPDPERFDPHRPEPENVFAYIPFGGGQRK
                     CVGNAFAILQVKTIFGTLLKEFEFELAGPPGEYREIMPSLILRPSDPCVLRYRRRKH"
BASE COUNT          799 a         1745 c         1571 g          808 t
ORIGIN      
        1 taggccccga gcacgtactc gagccccccg tccatcagct tgccgagcag ctggaactcc
       61 tgcgagaact ggtcctgctc gttcaggaac ggcgcattgg cgaagagccg cgtggtgacc
      121 gggttggcgc catagtcctg gtagatgtcc tgctcgacct cgcggtagcc ggtcagcgac
      181 ttcaatgtca ggtcatcgct gatttcccac gccagcgtca gggagtggcc gctgacctcg
      241 acattgctct cctcgaaggg cacgcggggt ctcacgttgt cctggcggtc agacctgaag
      301 ggaacgctct cgaaaccgaa ctgcgaggga ccgatgttct ggtagtagat cgtcccggaa
      361 tcgacatcgg agtagtcgta ggcgtagtcg gcgctgaagt tgtcgctcgg cgaccagttg
      421 atcgcgagac gtccggccgc gctgtcgttg tcgccgaagt tcgggccccg gccatcgttc
      481 tcgatccagc catccttttc cgatttcatc aggctcagct tcgcggcgag acccgccacg
      541 cgcgctgtat cgacttcggc gcgcgccttg acgtagtcgc ggtcgcccgc gctcagtccg
      601 cccttgaagc cccactcgcc cttgggcttc gcggtgatca tgttgatcgc gccgccgatg
      661 gtattgcggc catagaggct gccctgcggg ccgcgcaaca cctcgatgcg ctccaggctg
      721 gcgacctcgc tgcccagacc gatcgaacgg gccatgtaga taccgtcgat gtagacgccg
      781 acgctggggt cggtggacat gccgacatcg ttgttgccca cgccgcgcat gtaggtacgc
      841 agcgaggcct ggctgttggg gaactggcgc atgaacaggt tcggcacgct gaactggatg
      901 tcgccgaggt catcgacgtt cagcttctcg agcatgtcgg ccccgaaggc cagcaccgag
      961 atcggcgtgt cctgcagcga ggcctcgcgc cgctcggcgg taacgatgac ctcctcgagt
     1021 cccgtggccc gcgcgccgtc ttgctgcgcc tgcgcccatg gagcggcggc cccggccacg
     1081 gccattgcca gcacggtcgg cgcgaatctg cgtatccgga ttgccatgat gcgttcccct
     1141 cgttactgaa gttgttctgt atgaatcgaa ttcgcggatg ctgtcccgca ccctgcatcg
     1201 ggcaggcacc ggccgggcag gcatcgcccg ggcccgcttc gtccccgcca gacaatgcta
     1261 gacgctggac tcgaataaaa cattcaacag caatggtatt tttggcatcc cgcacgcctg
     1321 ctcacaaggc cgtgcccgcg cgcatgccat cctccaccgc cttgcgcagc aacccgggac
     1381 cggtgcaatc gccgatggcg tggatctccc cgacacgccc ggccagcgca tccgccaggc
     1441 gcgtgtcggg cgccgggctg ccggcaatga tcaccgtgtc cgccggcacg ctgccggcac
     1501 gcccggcata ctcgaaacgc accgcaccgg cgtcgatacc tgtcagccgc acaccgccga
     1561 gcacctcgac gccgaggcgg tcgagcgcgc tcaactgctc ctggcgacgc ttgggcccga
     1621 catcctgcgc gaaccactgg tcttcggcga tgaggctcac gcgccgaccg cggtgcgcga
     1681 tgaaatccgc gagttccacc gccggcaggt ccgaaccgat cacgcacacc cgcttgccga
     1741 gcggcaggaa gtgccggctc gccgcgcgca catgacccgg acgcagccac tgctgtacgc
     1801 gtggcatcac gtgatgaccg agcagcttca gcagcgcggg ccagcgcgcg gcttcttcgg
     1861 ccgacagcgt gcccgagaac cagccgcgca cctcggtcgc gtcgtagacg tgcgccgcct
     1921 ccatccccgg gatcaccggc cggaccatcc ttgcgcccgt cgccacgacc accgcgtcgg
     1981 catccgcgca ctccgcgggc tccagcgcgc ggcccagctc caccctgacc ggcaggcggc
     2041 tcatcgcgcc gagcaggtag tcgagcaggc gctggttgtc cgcgtgcacg atcgaggcat
     2101 agaacagcga gccaccgagc cgtccgccgc gctcgaccag gcgcacctca tggcctcgca
     2161 gcgccgcgac gcgcgcggcc tccatgcccg ccggcccgcc gccgacgacc agcacgcgct
     2221 tgcgcacggc ggccggcgtg atcgccagct ccgcttcgcg cccgctctgt gcgttcacgg
     2281 cgcagttcat gttggcgtcg aagggcgcga tcagcataga gtcgatgcag ttctggcacg
     2341 acaggcagcg gcggatctcc agcgtgcgac cgctcagcac cttcgcgggc aactccgggt
     2401 ccgccagcag cgggcgcgcc attgccacca tgtcggcgtc accctcggcg atgatcttct
     2461 cggcgaggac cgggtcgtgg atgcgcccca ccaccatcac cggcacgttc acgaccttgc
     2521 gcaccgcggc cgcgacggcg gtgttgtagc cgtcgcggta atgcgctccg cagacgatct
     2581 gcgccacgat cttgtcgtgc gagacgccgc cgctcacctg gaaggcgtcg atgccggcct
     2641 gctccagcaa cggcgcgatc agctgcgtgt cgttcagcat gcgcccgtcg aaggcatcct
     2701 cgttgccgga gatgcgcagc gttatcggga agtccgcacc ggcgcgctgc ttgatgtggc
     2761 ggatcacgcg caccagcagg cgcgtgcgcc cctcgatggt ggcgccggtg tactcgtcct
     2821 cgcggcggtt gcgcaccggg cacaggaagc tgcccagcag catgtagccg tgcgccgcgt
     2881 gcagttccat gccgtcgtag ccggcctcgc gcgcgcgcac cgcggcgtcg ccatactgcg
     2941 cgatcacgcg ctccatttcc tccgcttcga gcacccggct cggccagccg gtaccggcgt
     3001 tcacgttgac cgacggcccc accgggtcgg tcatcgactc gggaccggga tgggagatct
     3061 gcggctggat gcgcgcgccg tgggcatgca cccgccgcac cagctcgcgg tgtccggcga
     3121 tgaagcggtc atcgccgagg ctgagcgact gcggctggta gcgctgggca acatcgacgg
     3181 agcacatctc caccgtgatc agtcccacgc cgccgcgcgc gcgcgcctcg tggtagcgga
     3241 tcaggcgctc ggtcacctcg aagtccgcgc tgccgtaatt ggtggtcatc gccgacatga
     3301 ccatgcggtt gccgaccgtc atcgcaccga tgcggatcgg gcttgccagg tgctgaaagg
     3361 attgcatgct ggtttttctc ctaggcatca tcgcccttgt catggtagcc ggcctggcgt
     3421 agtataaaaa caattcaaca ccaatctttt tacacagagg gcagccccat gagccagagc
     3481 agcgacgcga gccggtatcg cccgatccca ttcgtcagcg gcgccgttcc cggcgggggc
     3541 cacttcgagg agttctgcgc ccacccggct tttttcctgc aacgcgcctt ccgcgagtgc
     3601 ggcgaggtct gcgagttcga ccagggcggc atgcgcacgg tgctgatggt gggccccgag
     3661 gcacacgaag ccgtgttccg cgcccccgac gagcagttga gcgctccggc cgcctaccag
     3721 tacatggtgc cgatcttcgg cccgggcgtg cagtatggcg ccccgatcga gatcgaacgc
     3781 cagcagctga agatgcacgc caagggcctg cgcgccgagc gcctgaccta ctacgcgccg
     3841 atcgtcgcca gggaggtcga ggactggatt tccggctggg gcgacgaggg cgagatggac
     3901 ttctacgagg ccttcgccga tctcacgatc aagacctcca cgcactgcct gctgggcgcc
     3961 gaattccgct actcgctgac cgatgagttc gcgcgccact accacgacct cggcgaggcc
     4021 gccgatgctg ccggcacggt cgatcacacc cagcagaagg ccgtctacga ccgccgcgac
     4081 cgggcgcgca aggcgctggg ggagttgatc atcgagcgca ttcaccgccg ccgcaacgcc
     4141 ggcgagacgc acgacgacct gctgcaggtc tacatggatg ccaccctgct cgacggcagc
     4201 cgcctcagcg acgagcagat cgccggcatg gtcgtctggt tcatgttcgc cgggcatcac
     4261 accagcgcga acaccgccgc atggaccctg gtggaactcg cgcgctaccc ggacttcggc
     4321 cgggaagtga ccgccgaggt cgaccagctg ttcgcgaccg agaccgagtt gagcttccgc
     4381 gcactgcgca agatgccgct catggagggg ttcatccgcg agatcctgcg cgcgcacccg
     4441 ccgctcaacg ccctgtgccg gcgcgtgatg caggatttcc actacaagga ctacctgatc
     4501 gaggccggca agaacgtgat gctctcgccc tacgtcgcgc accggctgcc acagtacttc
     4561 cccgacccgg aacgcttcga tccgcatcga ccggaaccgg aaaacgtctt cgcctacatc
     4621 cccttcggtg gcgggcagcg caagtgcgtc ggcaacgcct tcgcgatcct gcaggtgaag
     4681 acgattttcg gcacgctgct gaaggaattc gaattcgagc tcgcggggcc cccgggcgag
     4741 taccgggaga tcatgccctc gctgatcctg cgcccctcgg atccctgcgt cctgcgctac
     4801 cggcgccgca agcactgaaa ccacgacaac tgatgaacat ggagaaggca atggatttca
     4861 cgggcaaggt cgcgatcgtc acgggcgccg gcggcggcat cggcgcggcg accgcacgcc
     4921 ggc
//
LOCUS       DAORUQ010000217         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_21536_length_4870_cov_6.079543, whole genome shotgun sequence.
ACCESSION   DAORUQ010000217 DAORUQ010000000
VERSION     DAORUQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21536_length_4870_cov_6.079543"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..703
                     /locus_tag="PLW13_14225"
     CDS             236..703
                     /locus_tag="PLW13_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013972830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY71589.1"
                     /translation="MTVSAKPGRVPAITPYLSVRGAAAAIDFYRRAFDAEELYRLDAG
                     NGLVGHAEIRIGDSVILLADELSGGPQRSPASLAGTSVAVRLYVDDVDECFARALAAG
                     GVQLRGVADQFYGDRNGALRDPFGHVWIVGTQIESLTPEQIEARMQALRVATG"
     gene            727..2208
                     /locus_tag="PLW13_14230"
     CDS             727..2208
                     /locus_tag="PLW13_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY71590.1"
                     /translation="MSDFRDLQLLIRSHVPLLVVETHDETRARELLTRLAIKEALPLF
                     VWSVTEGLQRLGFGELPAAETLLEPDAVLKSIKAHREAGLFVLCDIHPYLADPRIVRL
                     LKDVALRYREVAHTLVLLSHRLAIPPELARLAARVELSLPDEEEIMAIVREEARRWQT
                     VNGGRRVKTDNRTLMQLTRSLRGLTHADCARLIRGAIFDEGSIAESALPEINRAKFEL
                     MDLDGVLSFEYRTEQFASVAGMQRLRAWLEQRREAAVAQGEAGALDRPKGVLLLGVQG
                     AGKSLTARAVAGAWGLPLLRLDMGALYNKYIGETERNLRESLRSAELMAPCVLWLDEI
                     EKGVGGSDADEGVSRRLLGSLLTWMAEQRDGVFVVATANDVSALPPELLRKGRFDELF
                     FIDLPDEATRAEIFRIHLRRRGIDPEGIDVGTLAGMSEGFSGAEIEQVVVSARYRAAA
                     DQAPLASAHLLTEIRATVPLAVTMAERIEALRAWAEGRTVPAN"
     gene            2252..2995
                     /locus_tag="PLW13_14235"
     CDS             2252..2995
                     /locus_tag="PLW13_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQY71591.1"
                     /translation="MQHEPHAFTGAQKVYLWLAMIFVASLLIADIVGIKLFRIPLPFT
                     LFGFDAIEHTCGMLTFPVTFLLTDLVNEYYGPRAARRITYLGLAAALFVFGVINLAQA
                     MPYLDAPYNVRKEQFDAIFGSAKVMYLASLCAYLVGQLSDIAVFGFFKRLTGQRFVWL
                     RATGSTVISQFIDSFVVSYLAFSLGRQIVADPALPPAPLSAIPAIAVTGYLLKFAIAI
                     AITPLIYAGRMVMHRWFGLTPIAVAPVKP"
     gene            complement(3174..4487)
                     /gene="purD"
                     /locus_tag="PLW13_14240"
     CDS             complement(3174..4487)
                     /gene="purD"
                     /locus_tag="PLW13_14240"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQY71592.1"
                     /translation="MNVLIIGGGGREHALAWKAAQSYRVDTVFVAPGNGGTAREPGVE
                     NVAIDVMDFEQLAQFARDNAVGLTIVGPEAPLVGGVVDHFRAAGLRCFGPTRGAAQLE
                     GSKAFTKDFLARHAIPTAEYRTFTEIAPAQAYIRERGAPIVVKADGLAAGKGVILADD
                     IDTAIAAVHDMLAGNAFGEAGHRVVIEEFLVGEEASFIVMVDGTNVLALASSQDHKAR
                     DDGDRGPNTGGMGAYSPAPVVTPAIHERVMREVIMPTVRGMAAEGNVYTGFLYAGLMI
                     APDGTPKVLEYNCRFGDPETQPIMMRLKSDITALCDAAIDGQLDAISAEWDPRAALGV
                     VMAAGGYPDSYRKGDVIEGLPEHAAADSHVFHAGTLLRYDDAVVTNGGRVLCVTALGD
                     TVAAAQQRAYAVCATIRWNGGFYRHDIGHRAIARERADAASPGSA"
     gene            complement(4508..>4870)
                     /gene="purH"
                     /locus_tag="PLW13_14245"
     CDS             complement(4508..>4870)
                     /gene="purH"
                     /locus_tag="PLW13_14245"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253541.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQY71593.1"
                     /translation="SEDEMRDLLFAWKVAKFVKSNAIVYARDNRTIGVGAGQMSRVNS
                     ARIAAIKAEHAGLAVDGAVMASDAFFPFRDGIDNAAASGIRAVIQPGGSMRDEEVIAA
                     ADEHGMAMVFTGMRHFRH"
BASE COUNT          701 a         1683 c         1691 g          795 t
ORIGIN      
        1 agcgccgggc tcgcgcgccc gggttgcggt gcctcgccgg tagcgaagcg gcgcgcccgg
       61 tcgagtccga tcacgagatg gtcgagcagt gaaaactggc gctgcatggg gaggctgctg
      121 acggttcgat gcgcggattc tatagtgcaa gcgcgctccc ggccaaacag ggtcaccgca
      181 gcggcgcggc gcgggtatga ttgggctatc cgcttgcacc gaggagacga taacaatgac
      241 ggtcagtgcc aagcccgggc gcgttcccgc catcacgccc tacctgagcg tgcgcggagc
      301 cgccgcggcc atcgatttct accgccgcgc cttcgatgcc gaggagttgt accgcctcga
      361 tgcgggcaac gggctggtgg ggcacgccga gatccgcatc ggcgactcgg tgatcctgct
      421 cgccgacgaa ctgagcggcg gcccgcagcg cagccccgcc tcgctcgccg gcacctcggt
      481 cgccgtgcgg ctgtacgtcg atgacgtcga cgagtgcttc gcgcgcgcgc tcgcggccgg
      541 cggcgtgcag ctgcgcggcg tcgccgacca gttctacggc gatcgcaacg gcgcgctgcg
      601 cgatcccttc ggccatgtct ggatcgtcgg cacgcaaatc gagagcctga cgccggagca
      661 gatcgaggcg cgaatgcagg cgctgcgcgt ggcgacggga tgatgcagga gccgcagccc
      721 gaggcgatga gcgacttcag ggatctgcaa ctgctgatcc gctcgcacgt gcccctgctg
      781 gtggtcgaga cgcacgacga gacccgtgcg cgcgagttgc tcacgaggct cgcgatcaag
      841 gaagcgctgc cgctgttcgt gtggtccgtg accgagggcc tgcagcggct cggcttcggc
      901 gagctgccgg ccgccgagac gctgctcgag cccgacgccg tgctgaaatc gatcaaggcg
      961 caccgcgagg cgggcctgtt cgtgctgtgc gacatccatc cctacctcgc cgatccgcgt
     1021 atcgtgcggc tgctgaagga cgtcgcgctg cgttatcgcg aggtggcgca cacgctggtg
     1081 ctgctcagcc accggctcgc gatcccgccg gagctcgcgc ggctcgcggc ccgtgtcgag
     1141 ctgtcgctgc ccgacgagga ggagatcatg gccatcgtgc gcgaggaggc gcggcgctgg
     1201 cagacggtga acggcgggcg gcgcgtgaag accgacaacc gcacgctgat gcagctcacg
     1261 cgcagcctgc gcgggctcac gcacgccgac tgcgcgcgcc tcatccgcgg cgcgatcttc
     1321 gacgagggca gcatcgcgga atcggcgctg cccgagatca accgcgccaa gttcgaactc
     1381 atggacctcg acggcgtgct gagcttcgag taccgcaccg agcagttcgc cagcgtggcc
     1441 ggcatgcagc gcctgcgtgc ctggctggag cagcgccgcg aggctgcggt cgcgcaaggc
     1501 gaggcgggcg cgctggatcg ccccaagggc gtgctgctgc tcggcgtgca gggcgcgggc
     1561 aagagcctga cggcgcgcgc cgtcgcgggc gcctggggcc tgccgctgct gcgcctggac
     1621 atgggcgcgc tgtacaacaa gtacatcggc gagacggagc gcaacctgcg cgagtcgctg
     1681 cgctcggccg agctgatggc gccctgcgtg ctatggctcg acgagatcga gaagggcgtg
     1741 ggcggctcgg acgccgacga gggcgtgtcg cgccgcctgc tcggcagtct gctgacctgg
     1801 atggccgagc agcgcgacgg cgtgttcgtg gtggcgaccg cgaacgatgt ctcggcgctg
     1861 ccgcccgaac tgctgcgcaa gggtcgcttc gacgaactgt tcttcatcga ccttcccgac
     1921 gaggcgacgc gtgccgagat cttccgcatc cacctgcgcc ggcgcggcat cgatcccgaa
     1981 ggcatcgacg tgggcacgct ggccgggatg agcgagggct tttccggggc cgagatcgaa
     2041 caggtggtgg tgagcgcccg ttaccgcgcc gcggccgacc aggcgccgct cgccagcgcg
     2101 cacctgctga ccgagatacg cgccacggtg ccgctggccg tcacgatggc cgagcgcatc
     2161 gaggccctgc gcgcgtgggc agaaggccgc acggtgcccg caaactgaac ccgacgtgcg
     2221 caacaccacc aacggagccc ggagtcgagc cgtgcaacac gaaccccatg catttaccgg
     2281 cgcccagaag gtctacctct ggctcgcgat gatcttcgtt gcctcgctgc tgatcgccga
     2341 tattgtcggc atcaagctgt tccgcatccc gctgccgttc acgctgttcg gcttcgacgc
     2401 gatcgagcac acctgcggca tgctgacctt cccggtcacc ttcctgctga ccgacctggt
     2461 caacgagtac tacggaccgc gcgcggcgcg acgcatcacg tatctcgggc tcgcggcggc
     2521 gctgttcgtc ttcggcgtga tcaacctcgc gcaggcgatg ccctacctcg acgcgccgta
     2581 caacgtgcgc aaggagcagt tcgacgcgat cttcggttcg gcgaaggtga tgtacctggc
     2641 atcgctgtgc gcctacctgg tggggcagtt gagcgacatc gcggtgttcg ggttcttcaa
     2701 gcgcctgacg gggcagcgct tcgtgtggct gcgcgccacc ggcagcacgg tgatctcgca
     2761 gttcatcgac tcgttcgtcg tgagctacct ggccttctcc ctggggcggc aaatcgtcgc
     2821 cgacccggcc ttgccgccgg cgccgttgtc cgcgattccc gcgatcgccg tcaccggcta
     2881 cctgctgaag ttcgcgatcg cgatcgcgat cacgccgctg atctacgccg gtcgcatggt
     2941 catgcaccgc tggttcgggc tgacgccgat cgcggtggct cccgtcaaac cgtagggcgg
     3001 cattcacgcc gacagcggcc gggttcggat gtcgccatga atggcgacct agggatgcct
     3061 ggccgggttt cgatgtcgcc atgaatggcg acctgcgggt gccatccgtg ggtccgcgtt
     3121 catgcggaca ttgtccggct gaagccggac ccacggaatc cggatacccg gtgtcaggct
     3181 gaacccggac tcgccgcgtc ggcgcgttcg cgcgcgatgg cgcggtggcc gatgtcgtgg
     3241 cggtagaaac cgccgttcca acggatcgtc gcgcataccg cgtaggcgcg ctgctgcgcc
     3301 gcggccaccg tgtcgccgag cgcggtgacg cacagcacgc gcccgccgtt ggtgaccacg
     3361 gcatcgtcgt agcgcagcag cgtgccggca tggaacacgt ggctgtccgc ggccgcgtgt
     3421 tcgggcagcc cctcgatcac gtcgcccttg cggtagctgt ccgggtagcc accggcggcc
     3481 atcacgacgc cgagcgccgc gcgcggatcc cactcggcgc tgatcgcgtc gagttgcccg
     3541 tcgatggcgg cgtcgcacag cgccgtgatg tcggacttca ggcgcatcat gatcggctgc
     3601 gtctccgggt cgccgaagcg gcagttgtac tccagcactt tcggtgtgcc gtcgggggcg
     3661 atcatcagcc ccgcgtagag aaacccggtg tagacgttcc cctcggccgc catgccgcgc
     3721 acggtgggca tgatgacctc gcgcatcacg cgttcgtgga tcgccggcgt gaccaccggt
     3781 gccggcgagt aggcgcccat gccgccggtg ttcgggccgc ggtccccgtc atcgcgcgcc
     3841 ttgtggtcct ggctgctcgc gagcgccagc acgttcgtgc cgtcgaccat cacgatgaaa
     3901 ctcgcttcct cgccgaccag gaattcctcg atcaccacgc ggtggccggc ttcgccgaag
     3961 gcgtttcccg cgagcatgtc gtgcaccgcc gcgatcgcgg tgtcgatgtc gtcggccagt
     4021 atcacgccct tgccggccgc cagcccgtcg gccttcacca cgatcggcgc gccgcgctca
     4081 cggatatagg cttgcgccgg cgcgatctcc gtgaaggtgc ggtactcggc ggtggggatg
     4141 gcgtggcgcg ccaggaagtc cttggtgaag gccttcgagc cctcgagctg cgccgcaccg
     4201 cgcgtcgggc cgaagcagcg cagccccgcc gcgcggaaat ggtccaccac gccgccgacc
     4261 agcggcgcct cggggccgac gatggtgagt cccacggcgt tgtcgcgcgc gaactgcgcg
     4321 agctgctcga agtccatcac gtcgatcgcg acgttctcca caccgggctc gcgtgccgtg
     4381 ccgccgttgc cgggggccac gaacacagtg tccacgcggt aactctgtgc cgccttccat
     4441 gccagcgcat gctcgcgccc gccgccgccg atgatcagga cgttcattgt tcgcttcctc
     4501 gcgcggatca gtgccggaag tggcgcatgc cggtgaatac catcgccatg ccgtgctcgt
     4561 cggcggcggc gatgacctcc tcgtcgcgca tcgagccgcc gggctggatc accgcgcgga
     4621 tgccggacgc ggcggcgttg tcgatgccgt cgcggaacgg gaagaaggca tccgatgcca
     4681 tcacggcgcc gtcgaccgcc agtcctgcgt gctcggcctt gatcgcggcg atgcgcgcgg
     4741 agttcacgcg gctcatctgt ccggcgccga cgccgatggt gcggttgtcg cgcgcgtaga
     4801 cgatcgcgtt cgacttcacg aacttcgcca ccttccacgc gaacagcagg tcgcgcatct
     4861 cgtcttccga
//
LOCUS       DAORUQ010000218         4789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_22083_length_4789_cov_5.587030, whole genome shotgun sequence.
ACCESSION   DAORUQ010000218 DAORUQ010000000
VERSION     DAORUQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22083_length_4789_cov_5.587030"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..813)
                     /locus_tag="PLW13_14250"
     CDS             complement(172..813)
                     /locus_tag="PLW13_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY71594.1"
                     /translation="MPKAPVSPHIGVLVVDDQYIVRRGIISTLADIPGMRCIGEAACG
                     EEAITLARALLPDVVLMDLRMPGIGGLEAARRIGIALPATRIIAVTAWDSEPSQRLWR
                     SRIEACVSKDVRAPELEATIRRVLAPRHRVAAPDEASLAANPFDRLSSREMQVCALLL
                     AGRRAPQIGLEMFIAVKTVHTFRYRIFGKLGVCGDIELAKLAASHGLIGGQPA"
     gene            complement(965..1558)
                     /locus_tag="PLW13_14255"
     CDS             complement(965..1558)
                     /locus_tag="PLW13_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HQY71595.1"
                     /translation="MNGDCERFDPGDGALALFAQAFVAEAPAWFAALMAEVRWEQHWL
                     TLFGRRIAAPRLSAWYAEPGCGYRYSGLSLVAQPFTPVLEQIRARTEALSGHGFNSVL
                     LNLYRDGADGMSWHSDDEPELGPAPLIASLSLGATRRLLLRRRDAHEVRFGLDLSDGS
                     LLVMEPPLQRHWQHSVPKTRRPMGPRINLTWRRILAR"
     gene            complement(1560..2567)
                     /gene="murB"
                     /locus_tag="PLW13_14260"
     CDS             complement(1560..2567)
                     /gene="murB"
                     /locus_tag="PLW13_14260"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQY71596.1"
                     /translation="MRIEADVPLAHCNTLRLPARAEWCATVESGDELREALDFARTRR
                     LALCVLGGGSNVVLRERIGGCVLRMQLQGEELLEETPGQVRVRVAAGVQWHGFVLACH
                     RRGWHGLENLALIPGTVGAAPIQNIGAYGVEVQEFVEAVGAVERASGEALRLSHADCR
                     FAYRHSIFKSELAERIVITDLTLRLPRARAPRSDYPVLRAALGAVADPSPADVLEAVM
                     RIRRERLPDPALLPNAGSFFKNPVIDRARFERLRAGHPGVVHWAAGDAVKLAAAWLVE
                     QAGGKGMREGAARVHERQALVLVNEGGASAAQVLGLAARIATAVRERFGIDLEIEPAV
                     Y"
     gene            complement(2601..2720)
                     /locus_tag="PLW13_14265"
                     /pseudo
     CDS             complement(2601..2720)
                     /locus_tag="PLW13_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2845..>4789)
                     /locus_tag="PLW13_14270"
     CDS             complement(2845..>4789)
                     /locus_tag="PLW13_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71597.1"
                     /translation="LANRRRLVVFTGVFAVLCVLSLAYTFLRAPQYRALALLQITPGA
                     AAPRPIETVSVQSAAPGAESDKPFLTEVQVLGSRPVLEQTVARLARDGHDLSSLGEDP
                     VAAMQAMIQVSPVSGTAVVEMSATGAQAGLLASLLNTAVDAYREHLGATARESIAEVL
                     AGSDEEIGKLERRVAEKNDEVEAFRIAHDIVSLEREENQVLARVRGVAASLNTANERV
                     AVAEGRLRSVSEAMAAGSAVTRSRDDPTLANLEQRASQIREDLGELERNYTPDYMSLD
                     PNIKAKRDRLAELERQIAAQRGLNAQAALAEAREELASAREMAARIRQQEASDREAVQ
                     DFTARFNEYKALQDELTELQTLYRAATQRKVRLEASERERMPSVQVLEAATTPQTAWR
                     PLYARDAGIAVAGSFVLALLAMWFVELFNRPGPQPAVLITQPGGPLLGAAAPVAALAG
                     AANRMLGVPERALLPEGPVLPAELAPGEVAELLHAATAEGRLGMLLILSGASPEEALA
                     LSWDDVDLERQVVAIRGDSARELPLSASLRAALGAREACPGAPLLADAQGRGLAMDGL
                     HAAILCAAHDAGIGHAGVVTPAALRHTFIAFLVRQGVRFADLAQLVGRLPAETLVAYS
                     SLSPAGPRVPLEQVERVMPALQV"
BASE COUNT          706 a         1764 c         1660 g          659 t
ORIGIN      
        1 ggcgtcgtac atctggtaga caccgggcag ctcggtgaga ttgcgcagga aagaggccgc
       61 gtcgaagccc gtcacgatga ttccccggtc gagccgcgct ctccacggcg cggcgcacgg
      121 gattatagcc gtccgcgcac ggcgcccgcg cggcggcgcc gcgccagcct ctcaggcggg
      181 ttgcccgccg atcagcccgt ggctggcggc gagcttcgcg agctcgatgt caccgcagac
      241 gccgagcttg ccgaagatgc ggtagcggaa ggtgtgcacc gtcttgacgg cgatgaacat
      301 ctccagcccg atctgcggcg cgcgacggcc cgccagcaac agtgcgcaga cctgcatctc
      361 gcggctgctc agccggtcga agggattcgc ggccaatgac gcctcgtcgg gcgccgcgac
      421 ccggtggcgc ggtgccagca cgcgacggat cgtcgcctcc agttcgggcg cgcgcacgtc
      481 cttgctcacg caggcctcga tgcggctgcg ccacaaccgc tgcgacggct cgctgtccca
      541 ggcggtcacc gcgatgatgc gcgtcgcggg caacgcgata ccgatgcgcc gcgccgcctc
      601 cagcccgccg atgccgggca tgcgcaggtc catcagcacg acatcgggga gcagggcccg
      661 cgcgagggtg atggcctcct cgccgcacgc agcctcgccg atgcagcgca tgccgggaat
      721 atcggcaagg gtactgatga tgccgcggcg cacgatgtac tggtcatcca ccacgagcac
      781 gccgatgtgg ggcgatacgg gagctttcgg cacacctggt ccctccaatc ggtgcaggga
      841 acgccgctgt actgcctgct cgttggcgtc tgcaatcacg gggaaccgac gctattacag
      901 ctcccgccac gccgtcgagt cccattcggg ctacgaccga cgcgccgagg ggcgaacgcg
      961 acgctcagcg cgccaggatc cggcgccagg tcaggttgat gcggggcccc atgggacggc
     1021 gggtcttggg cacgctgtgc tgccagtgac gctgcagcgg cggctccatc accagcagcg
     1081 aaccgtcgct gaggtcgaga ccgaagcgca cttcgtgcgc atcgcgccgg cgcagcagca
     1141 gccgccgcgt ggcgcccagg ctcagggagg cgatcagcgg cgccggcccg agctcgggtt
     1201 cgtcgtcgct gtgccaggac atgccgtcgg cgccgtcgcg gtagaggttc agcagcacgc
     1261 tgttgaagcc gtggccgctc aacgcttcgg tgcgcgcgcg gatctgctcg agcaccggcg
     1321 tgaagggctg cgccaccagg ctgagtcccg aataacggta gccgcaaccg ggctcggcat
     1381 accacgcgct caggcgcggc gcggcaatgc gccggccgaa cagcgtgagc cagtgctgtt
     1441 cccagcgcac ttccgccatc agcgcggcga accatgccgg cgcctcggcg acgaaggcct
     1501 gcgcgaagag cgccagtgcg ccgtcgccgg gatcgaagcg ctcgcagtcg ccgttcatct
     1561 cagtagaccg ccggctcgat ctcgagatcg atgccgaagc gctcacgcac cgccgtggcg
     1621 atgcgcgcgg ccagccccag cacttgcgcc gcgctggcgc ccccctcgtt gaccagcacg
     1681 agcgcctggc gttcgtgcac ccgcgccgcc ccctcgcgca tgcccttgcc accggcctgc
     1741 tccaccagcc acgcggccgc gagcttcacg gcgtcgccgg ccgcccagtg caccacgccg
     1801 ggatgcccgg cgcgcaaacg ctcgaagcgc gcgcggtcga tgaccgggtt cttgaagaag
     1861 ctgccagcgt ttggcagcag cgcgggatcg ggcaggcgct cgcggcgtat gcgcatcacc
     1921 gcttccagca cgtcggcggg cgaaggatcc gcgacggccc ccagcgcggc gcgcagcacc
     1981 gggtaatcgc tgcgcggcgc gcgcgcgcgc ggcaggcgca gcgtgaggtc ggtgatgacg
     2041 atgcgctcgg ccagttcgct cttgaaaatg ctgtgacggt acgcgaagcg gcagtcggca
     2101 tgcgagagcc gcagcgcctc tccgctcgcg cgctccaccg cgccgaccgc ctcgacgaac
     2161 tcctgcacct cgacgccgta ggcgccgatg ttctggatcg gcgcggcgcc gacggtgccg
     2221 gggatcagcg cgaggttttc cagcccgtgc cagccgcggc ggtggcaggc cagcacgaag
     2281 ccatgccatt ggaccccggc cgcgacgcgc acgcgcacct gcccgggggt ttcctccagc
     2341 agctcctcgc cctggagctg catgcgcagc acgcagccgc cgatgcgctc gcgcagcacc
     2401 acgttgctgc cgccgccgag cacgcacagc gcgagccggc gcgtgcgcgc gaaatccagc
     2461 gcctcgcgca actcatcgcc gctctcgaca gtggcgcacc actcggcacg cgcgggcaga
     2521 cgcagtgtgt tgcagtgcgc gagcgggacg tcggcctcga tgcgcatgga tcagagacgc
     2581 gtgcggcgga tgtgcgccgg cagcggcgac tgcagcccga gcgcctcgac cttgcgcccc
     2641 agcaccgcat cggcggtggg cgatcggcag atgttgccca ggcaaacgaa aagtaccgaa
     2701 acgcgctgac tgtcccgcat cgaggctctg cttcccgtga aaaacgcgat ggcgcgcagt
     2761 gtatccgatc gccgccgggc ccttcgaccg cctgcgaatg caggccatcg ccatctcgct
     2821 ccggaatgcg gcaattcgga tcgatcagac ctgcagcgcg ggcatcacgc gctcgacctg
     2881 ctccagcggc acgcgcggac ccgccggcga aaggctgctg taggcgacca gggtctcggc
     2941 cgggagccgg ccgacgagtt gcgcgagatc ggcgaagcgc acgccctggc gcaccaggaa
     3001 ggcgatgaag gtgtgccgca gcgccgcggg cgtcaccacg ccggcatgcc cgatgccggc
     3061 atcgtgcgcc gcgcagagga tcgcggcatg cagcccgtcc atggcgagcc cgcggccctg
     3121 ggcatcggcc agcagcggcg cgccggggca ggcctcgcgc gcgccgagcg ccgcgcgcag
     3181 cgacgcgctc agcggtaact ctcgcgccga atcgccgcgg atggccacaa cctgccgctc
     3241 gaggtccacg tcgtcccagc tgagggccag ggcctcctca gggctggcgc cgctcaggat
     3301 cagcaacatg ccgagccggc cctccgcggt ggcggcatgc agcagttcgg ccacctcccc
     3361 gggcgccagc tcagccggca gcaccggccc ctccgggagc agcgccctct cggggacccc
     3421 gagcatccgg ttggccgcac cggccagcgc ggcaaccggt gccgcggcgc cgagcagcgg
     3481 cccgccgggc tgcgtgatca gcaccgcggg ctgcggcccg gggcggttga agagctcgac
     3541 gaaccacatc gccagcagcg ccagcacgaa ggagcccgcg accgcgatgc cggcgtcgcg
     3601 cgcatagagc ggacgccatg ccgtttgcgg cgtggtcgcg gcttccagca cctgcaccga
     3661 gggcatgcgc tcgcgctcgc tcgcctccag tcgcaccttg cgctgcgtcg ccgcgcggta
     3721 gagcgtctgc aattccgtga gctcgtcctg aagcgccttg tactcgttga agcgggcggt
     3781 gaaatcctgc accgcttcgc gatccgaggc ctcctgctgg cgtatgcgcg ccgccatctc
     3841 gcgggcgctc gccagctcct ccctcgcctc ggccagcgcg gcctgcgcgt tcaggccgcg
     3901 ctgcgccgcg atctggcgct ccagctccgc gaggcgatcg cgcttggcct ttatgttggg
     3961 atccaggctc atgtagtcgg gcgtgtagtt gcgctccagt tcgcccagat cctcgcgtat
     4021 ctgcgaggcg cgctgctcga ggttggccag cgtcggatcg tcgcgcgaac gcgtcaccgc
     4081 gctgccggcg gccatcgcct cgctgaccga acgcagtcgg ccctcggcga ccgcgacgcg
     4141 ttcgttggcc gtgttcagcg aggccgcgac tccgcgcacg cgcgcgagca cctggttctc
     4201 ctcgcgctcg agcgacacga tgtcgtgcgc gatcctgaac gcctcgacct cgtcgttctt
     4261 ctcggcgacc cggcgctcga gtttcccgat ctcctcgtcc gagcccgcca gcacctcggc
     4321 tatggactcg cgcgccgtcg cgcccaggtg ttcgcgatag gcatcgaccg ccgtgttcag
     4381 cagcgacgcc agcaatccgg cctgcgcgcc cgtcgcgctc atctcgacca ccgcggtacc
     4441 gctcacgggg ctcacctgga tcatcgcctg catcgcggcg accgggtcct cgccgagtga
     4501 cgaaagatcg tgaccatcgc gcgccagacg cgcgacggtc tgctcgagca cggggcggct
     4561 gccgagcacc tgcacctcgg tcaggaaggg tttgtcgctc tcggcgcccg gcgcggccga
     4621 ttgcaccgac acggtctcga tgggcctcgg cgcggcggcg ccgggcgtga tctgcagcaa
     4681 cgccagcgcg cggtattgcg gcgcgcgcag gaaggtgtaa gcgaggctga gcacgcagag
     4741 cacggcgaac acgccggtga acacgaccag ccgccggcga ttggcgagg
//
LOCUS       DAORUQ010000219         4739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_22461_length_4739_cov_6.016439, whole genome shotgun sequence.
ACCESSION   DAORUQ010000219 DAORUQ010000000
VERSION     DAORUQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22461_length_4739_cov_6.016439"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2471)
                     /locus_tag="PLW13_14275"
     CDS             complement(<1..2471)
                     /locus_tag="PLW13_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQY71598.1"
                     /translation="MKRMLINATQPEELRVALVDGQRLYDLDIENRNRVSTKANIYKG
                     RITRVEPSLEAAFVEFGADRHGFLPLKEISPEYFQGNGGDQSQGRPRIRDVVKEGTEV
                     LVQVDKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGDERTELRDALNAL
                     NLPDGMGIIIRTAGVGRSAEELQWDLDYLLHLWGAIAEAGKKRAPQLIYQESNVVIRA
                     IRDCLRQDIDEVLIDAQDAHDKAVEFIEQVMPNYRSRVKLYKDPVPLFNRYQIESQIE
                     TAYQREVKLPSGGSIVIDPTEALVSIDINSSRATKGQDIEETALQTNLQAADEIARQL
                     RLRDMGGLIVIDFIDMNSTRNQKEVENRLRDALVPDRARVQVGRISRFGLMEMSRQRL
                     RPSLEETSSVVCPRCTGQGTIRDTKSLALSVLRLVEEEAGKERSAQIRTIVPVEIATY
                     LLNEKRRAIIDIEQRNRLHVMVIPNPNMETPHFEVMRLRDDSALVNSEEPSFLSIPEA
                     PPPVDLVTRTAEPVKVPVAAVQSIAPSKPAPVAAPEPVAQAAPAPVVAPAPVAAAPAA
                     QARPGIVKSLLGFLFGGGAAEPAPAARTAPAPRPAEEARRTPEGATAGERARGEGARA
                     RGEGEPGEGERRSGGRRRRGGSGRGRGGEREAAPGEAPRGESRGEPRGEPRAQAPRSE
                     RAPRPPRDESAGESRPPRGEGRPAQRPEGSPERRPAGQQPRTRGPRQRGEVQAPGSVA
                     EPAVAEVARTPMPPMPMQAAEPQPEVEEIVAAQAVVDEAQEAAPGTEGERSRRSRGRR
                     GRGRRRVEGEPVETVFQGEGDEPEAA"
     gene            3038..3988
                     /gene="rluC"
                     /locus_tag="PLW13_14280"
     CDS             3038..3988
                     /gene="rluC"
                     /locus_tag="PLW13_14280"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HQY71599.1"
                     /translation="MSELSAREQQGVRLVEVDAEHAGQRLDNFLLARLKGVPRSRIYR
                     IVRSGEVRVNSGRARPDQRLQAGDKVRVPPVRMAERAAAEPGVGLVQALATRILYEDA
                     ALLVVDKPSGLAVHGGSGLSLGLIEALRAMRPDCRSLELVHRLDRDTSGCVMIAKKRS
                     MLRHLHEALREGTITKTYITLVQGRWSKRLTRVTLALEKNTVRGGERMVRADEAGKAS
                     ETRFRVLEVLRDATLLEAQPVTGRTHQIRVHAQVSGHPVAGDEKYGEREFNRQMREFG
                     LRRLFLHAGSIELSLPDGQPLVVSAPLDVALTKVLETLPR"
     gene            3985..4638
                     /locus_tag="PLW13_14285"
     CDS             3985..4638
                     /locus_tag="PLW13_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY71600.1"
                     /translation="MRRLFVFDWDGTLMDSVGRIVSCLRQAADEQGLADLGDAAFGDV
                     IGLGLPQAIAQLYPQLDQRQIEQFRATYAARFVAADGRPSQLFPGALELLEALRDRGH
                     WIAVATGKSRRGLDRVLGELGLSDSFHATRCADETASKPDPRMLHEIVAQLGAEPEQT
                     VMIGDTEYDLEMAARARIRSLGVSHGVHSRERLARHAPARIVDSLPEILDWEELRPR"
BASE COUNT          637 a         1575 c         1699 g          828 t
ORIGIN      
        1 gccgcttcgg gctcgtcgcc ttcgccctgg aacacggttt cgacgggctc gccttcgaca
       61 cgccggcgac cgcgaccacg gcgtccgcgc gaacgacggc tgcgctcacc ctcggtgcca
      121 ggggctgctt cctgcgcctc gtccaccact gcttgcgcgg ccacgatttc ctcgacctcg
      181 ggttgcggtt ccgccgcctg catcggcatc ggcggcatcg gggtccgcgc gacctcggct
      241 actgccggtt cggcaacgct tcccggtgcc tgcacctcac cacgctggcg cgggccgcgt
      301 gtgcgcggct gctggcctgc cgggcggcgt tcgggactgc cttccggacg ctgtgccgga
      361 cggccttcgc cgcgcggtgg tcggctttca ccggcggact cgtcacgcgg tgggcgcggc
      421 gcgcgttcgc tgcgcggcgc ttgggcgcgt ggctcgccac ggggttcgcc acgtgattcg
      481 ccgcgcggtg cttcgccggg cgctgcttcg cgctcgccgc ctcggccgcg gcccgatccg
      541 ccgcggcggc gtcgaccgcc actgcggcgc tcgccttcgc cgggttcgcc ttcgccgcgc
      601 gcgcgtgcgc cttcgccacg tgcgcgctcg cccgcggtgg cgccttcggg cgtgcgtcgg
      661 gcttcttcag cggggcgcgg cgcgggcgcc gtacgggcgg ccggcgcggg ttcggccgcg
      721 ccgccaccga acaggaagcc cagcagggat ttgacgatgc cgggcctggc ttgcgccgcg
      781 ggtgccgcag ccaccggagc gggtgcaacg acaggggcgg gggccgcttg cgcgaccggc
      841 tcgggagccg ccaccggcgc aggcttcgac ggtgcgatcg actgcaccgc agccaccggt
      901 actttcacgg gctcggccgt gcgcgtgacc aggtccacgg gcggcggcgc ctcggggatc
      961 gagaggaagc tcggctcctc gctgttcacc agcgcgctgt cgtcgcgcag gcgcatcacc
     1021 tcgaagtgcg gggtctccat gttcgggttc gggatcacca tcacgtgcag gcggttgcgc
     1081 tgctcgatgt cgatgatcgc gcgacgcttc tcgttcaaca ggtaggtcgc gatctcgacg
     1141 ggcacgatgg tgcggatctg cgcactgcgt tccttgccgg cttcctcttc caccaggcgc
     1201 agcaccgaga gggcgaggga cttggtgtcg cggatggtgc cctggcccgt gcaacgcggg
     1261 cagaccacgg agctggtttc ctcaagcgag gggcgcaggc gctggcgcga catttccatc
     1321 aggccgaagc gcgagatgcg cccgacctgc acgcgggcac ggtcgggcac cagcgcgtcg
     1381 cgcaggcggt tctcgacttc cttctggttg cgcgtgctat tcatgtcgat gaagtcgatc
     1441 acgatcaggc cgcccatgtc gcgcaggcgc agctggcgcg cgatctcgtc ggccgcctgc
     1501 aggttcgtct gcagtgcggt ctcctcgatg tcctggccct tggtggcgcg cgaggagttg
     1561 atgtcgatcg acaccagcgc ctcggtcggg tcgatcacga tcgagccgcc tgagggaagt
     1621 ttcacctcgc gctggtaggc cgtctcgatc tggctctcga tctggtagcg gttgaacagc
     1681 ggcaccgggt ccttgtagag cttcacccgg ctgcggtagt tcggcatgac ctgctcgatg
     1741 aactccacgg ccttgtcgtg ggcgtcctgc gcgtcgatca gtacttcgtc gatgtcctgg
     1801 cgcaggcagt cgcggatcgc gcggatcacg acgttgcttt cctggtagat cagttgcggc
     1861 gcgcgcttct tgccggcctc ggcgatcgcg ccccacaggt gcagcaggta gtcgaggtcc
     1921 cattggagct cttcggccga gcggccgacg ccggcggtgc ggatgatgat gcccatgccg
     1981 tcgggcaggt tcagcgcgtt cagcgcgtcg cgcagctccg tgcgctcgtc gccctcgatg
     2041 cgacggctga tgccgccggc gcgcgggttg ttcggcatca gcacgaggta gcgacccgcg
     2101 aggctgatga aggtggtgag ggcagcaccc ttgttgccgc gctcttcctt gtcgacctgc
     2161 accaggacct cggtgccttc cttgaccacg tcgcggatgc gcggacggcc ctgcgactgg
     2221 tcgccgccgt tgccctggaa gtactcggga gaaatttcct tcagcggcag gaagccgtgg
     2281 cggtcggcgc cgaattcgac gaaggcagcc tcgaggctgg gctcgacgcg ggtgatgcgg
     2341 cctttataga tgttggcctt ggtcgagacg cggttacggt tctcgatgtc cagatcgtag
     2401 agtcgctggc catcgaccag ggcgacccgc aactcctccg gctgggttgc gttgatgagc
     2461 attcttttca tggttaccaa tgtcctgtga gcgggttgct gggactttgg taccgtcgtt
     2521 acagcactac ggaatcagtg gcttgtcatg cagaggcttc ttgcttcctc gaagtctgga
     2581 ttgcgcatat gcggttccgg gcgctacgcc ccgccgaatc gccttcgtcc cgagccgcca
     2641 gaaactgcct ggcggcaacg gggacgcgtc tcagtctttt ccggtccagc gggcggtgcg
     2701 aattcgcgac ccggcccgca caaaaatctc aacaagcatc ccgtcgctgt tcgaacggta
     2761 ccttgcgcct gccggtctcg cccctgcgaa accggccaca ccgcgcgcgc cgggctatgt
     2821 gattcctgct cagcggcgcg tcgtcggcgt gatgcatgaa acccgtgcgg gcgcccgggt
     2881 cggtgcgtcc cgcgcgccgc gttggtgcag cggtggcgtg gacgggtttc ctgatcctgg
     2941 gggcacgctc ctgcgaggca gcgggtccaa tgcggagcgg actatagcat tatcgggtaa
     3001 gtgcttcaat tagctgcaaa aatgatagac tttcgccatg tcagaactat cagcccggga
     3061 acaacagggc gttcgcctgg tcgaggtgga cgcggagcac gccgggcaac ggctcgataa
     3121 tttcctgctc gcgcggctca agggcgtgcc gcgctcgcgc atttaccgca tcgtgcgcag
     3181 cggcgaggtg cgggtcaaca gcgggcgcgc caggccggac cagcgcctgc aggccggcga
     3241 caaggtgcgg gtaccgccgg tgcgcatggc cgagcgggcc gctgccgagc ccggggtcgg
     3301 gctggtgcag gcgctcgcga cacgcatcct ttatgaggat gccgcgctgc tggtcgtcga
     3361 caagccgagc ggcctggcgg tgcacggcgg cagcggcctg tcgctgggat tgatcgaggc
     3421 gctgcgggcg atgcgcccgg actgccgctc gctggaactg gtgcatcggc tcgatcgcga
     3481 tacctccggc tgcgtgatga tcgccaaaaa gcgctcgatg ctgcgacacc tccacgaggc
     3541 gctgcgcgag ggcacgatca ccaagaccta tatcaccctg gtgcaggggc gctggtctaa
     3601 gcggctcacg cgggtgacgc tcgccctgga gaagaacacg gtgcgcggcg gcgagcggat
     3661 ggttcgcgcg gacgaggcgg gcaaggcctc cgagacccgc tttcgcgtgc tcgaggtgtt
     3721 gcgcgacgcc accctgctcg aggcgcagcc cgtcacgggg cgcacgcacc agattcgcgt
     3781 ccacgcccag gtctcggggc atcccgtggc tggtgacgag aagtacggcg agcgggagtt
     3841 caaccgccag atgcgcgagt tcgggctgcg tcgcctgttc ctgcatgccg gttcgatcga
     3901 gctcagcctt cccgatggcc agccgctggt ggtcagcgcg ccgctcgacg tcgcgctgac
     3961 gaaggtgctg gagaccttgc cgcgatgagg cgcctgttcg tgttcgactg ggacggcacg
     4021 ctgatggact cggtggggcg catcgtgtcc tgcctgcgcc aggcggccga cgagcagggg
     4081 ctggcggatc tgggggacgc ggccttcggg gacgtgatcg gcctggggtt gccgcaggcg
     4141 atagcgcagc tctacccgca gctggaccag cgccagatcg agcagttccg cgccacctac
     4201 gccgcgcgtt tcgtcgccgc cgatggtcgt ccctcgcaat tgttccccgg tgcgctggag
     4261 ctgctggagg cgctgcgcga ccgcgggcac tggatcgcgg tggcgaccgg caagagccgc
     4321 cgcggcctgg atcgcgtgct cggcgagctc gggctcagcg acagcttcca tgccacgcgc
     4381 tgtgccgatg agaccgcctc gaagcccgat ccccggatgc tgcacgagat cgtggcgcag
     4441 ctcggcgccg agccggagca gaccgtgatg atcggggaca ccgaatatga cctggagatg
     4501 gcggcacggg cccggatccg cagtctcggc gtgagccacg gggtgcattc gcgggaacgg
     4561 ctggcgcgtc acgcgccggc gcggatcgtc gattcgctgc cggagatcct cgactgggag
     4621 gagctgcgcc cgcgatgagc gcgggccgcg cgcatcaatg ctgcagtacg gccacgccga
     4681 agcgctcgag gaactccacc aggcggatca gcggcaagcc ctcgagcacg gtcggatcg
//
LOCUS       DAORUQ010000220         4734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_22494_length_4734_cov_5.896559, whole genome shotgun sequence.
ACCESSION   DAORUQ010000220 DAORUQ010000000
VERSION     DAORUQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22494_length_4734_cov_5.896559"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1542)
                     /gene="lnt"
                     /locus_tag="PLW13_14290"
     CDS             complement(25..1542)
                     /gene="lnt"
                     /locus_tag="PLW13_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQY71601.1"
                     /translation="MSFPARLAGPGIAGHAAALLAGALLPAALAPFDIWPLAVLCPLA
                     LLALMRRLRPDAAALRAWSFGVGMFGTGASWVYVSIHQYGSAPVPLAGVLTLLFCLGL
                     ALFPAFTFWAWARWLRERPGGVLFGFSAAWVLGEWWRGWFLTGFPWLYAGYSQLEAPL
                     AGWAPLIGVLGIGMILAFTAAALYLTLARSRGGPAALAASALLWTAGPLLERIDWTQP
                     VGEPLSVAMVQGNIAQELKWEPGQLEAILDTYVAMSEPLWGTDLVVWPEAAVPAYLDL
                     VVDRLAPLAARAVASHSTLLLGIPTRDANETKRRGFDAFNSIAVIGADSGLYHKRRLV
                     PFGEYVPLESVLRGLIAFFDLPMSSFSSGPAAQAPLTVRGLRVAPSICYEIVYPDLVG
                     SGAAGAELLLTVSNDTWFGASIGPLQHLQMARMRALETGRPLIRATNNGISALIDARG
                     GITVRGGQFTQEVIRGEVQPTSGLTPFARWGSWWALLLAVLPLVAAQLVRRRAPQ"
     gene            complement(1539..2396)
                     /locus_tag="PLW13_14295"
     CDS             complement(1539..2396)
                     /locus_tag="PLW13_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQY71602.1"
                     /translation="MSDDRNADEHDERSWLEKIAQVFSSDPRTRKDLLDILVIAEQND
                     VIDTETHGIMQGALEVAHLQVRDVMIPRPQMVCLHSDDAPGEVVRRVIESGHSRYPVT
                     GDSSDDVLGIVLAKDMLGQLFANGPGSIDIAQLLRPATFIPESKRLNVLLREFRENRN
                     HIAIVIDEYGGVAGLVTIEDVLEEIVGEIEDEHDKQQDTFIKKLSENDYLIKALTPIE
                     EFNKYFDAKLSDEEFDTIGGIVMQTFGHLPRRNEIAEIEGFHFKVLNADSRQIHLLRL
                     NLDPGASGG"
     gene            complement(2393..2803)
                     /gene="ybeY"
                     /locus_tag="PLW13_14300"
     CDS             complement(2393..2803)
                     /gene="ybeY"
                     /locus_tag="PLW13_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQY71603.1"
                     /translation="MRRWVRAALGAEHAEAELSVRIVDEPEMRELNARYRHKDYPTNV
                     LSFPAGLPPGVDVPLLGDIVLCAPVVNREAAEQHKAARAHWAHMLVHGTLHLLGHDHE
                     RARDAAVMEALEVRILAGLKFPDPYQEHEQAERP"
     gene            complement(2868..3893)
                     /locus_tag="PLW13_14305"
     CDS             complement(2868..3893)
                     /locus_tag="PLW13_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY71604.1"
                     /translation="MNDPQTLQFVLEPSDSRRLARLCGQFDQHLHLIEERLGVSIRNR
                     GNHFVVEGAEPARIAARAVIIHLYDETARHLELTPDQVHLRLQQSGVEELTAAEAAGE
                     GEVAPLGVVRLRRAVIKPRGKSQQHYVRSIHQHDINFGIGPAGTGKTYLAVACAVAAL
                     ESDQVRRILLVRPAVEAGEKLGFLPGDLAQKIDPYLRPLYDALYEMLGFETVSKLIER
                     HVIEVCPLAYMRGRTLNHAFVILDESQNTTPEQMKMFLTRIGFGSKAVITGDVTQVDL
                     PRGTQSGLAQVMTFLEGVEGIGFTWFTPRDVVRHPLVQRIVEAYEAFEKRLASEPAAP
                     RRGHTPQ"
     gene            complement(4075..>4734)
                     /locus_tag="PLW13_14310"
     CDS             complement(4075..>4734)
                     /locus_tag="PLW13_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQY71605.1"
                     /translation="HLVAAVEGIGRIRYTTSHPLEFSDALVQAYADIPQLVSHVHLPV
                     QSGSDRVLAAMKRNHTALEYKSTIRKLRRVRPDISISSDFIVGFPGETERDFEDTMRL
                     IADVGFDHSFSFVYSARPGTPAADLPDDTPEELKKQRLALLQARINQQAFEISRRMVG
                     GTQRLLVTEVSKRDPGRLQGRTENNRLTHFRSDDTGLIGQFVDVEITEALPNSLRAEV
                     L"
BASE COUNT          711 a         1604 c         1672 g          747 t
ORIGIN      
        1 gcgccggcgg tctgagccgg ggcgctattg cggcgcccgg cgacgcacca actgcgccgc
       61 caccagcggc agcacggcca gcagcagcgc ccaccacgag ccccagcgcg cgaacggcgt
      121 gagccccgac gtgggctgca cttcgccgcg gatgacttcc tgcgtgaact gcccgccgcg
      181 caccgtgatg ccgccgcgcg catcgatcag tgccgagatg ccgttgttgg tggcgcggat
      241 cagcggccgg ccggtctcga gcgcgcgcat gcgcgccatc tgcaggtgct gcagcgggcc
      301 gatcgaggcg ccgaaccagg tgtcgttgct gacggtgagc agcagttcgg cgccggcggc
      361 gccagagccg accaggtccg ggtagacgat ctcgtaacag atcgagggcg ccacgcgcag
      421 cccgcgcacc gtgagcgggg cctgtgcggc gggaccgctg ctgaaactcg acatcggcag
      481 gtcgaagaag gcgatcaggc cgcgcagcac ggattccagc ggcacatact ccccgaacgg
      541 caccaggcgg cgcttgtggt acagcccgga gtcggcgccg atcaccgcga tgctgttgaa
      601 ggcgtcgaat ccgcgtcgct tcgtttcgtt ggcatcacgc gtggggatgc cgagcagcag
      661 cgtgctgtgc gatgccacgg cgcgcgccgc gagcggcgcg agcctgtcca cgaccaggtc
      721 gaggtaggcc ggcacggcgg cctccggcca caccacgaga tccgtgcccc agagcggttc
      781 gctcatcgcg acgtaggtgt cgaggatcgc ctccagttgc ccgggctccc acttgagctc
      841 ctgcgcgatg ttgccctgca ccatggcgac cgacagcggc tcgcccaccg gctgcgtcca
      901 gtcgatgcgc tcgagcaggg ggccagcggt ccagagcagc gcgctggcgg cgagtgccgc
      961 cgggccgccg cgcgagcgcg cgagcgtgag gtagagcgcc gcggcggtga aggcgaggat
     1021 catcccgatg ccgagcacgc cgatgagcgg tgcccagccg gccagcgggg cctcgagctg
     1081 gctgtagccc gcgtagagcc aggggaagcc ggtgagaaac cagccgcgcc accactcgcc
     1141 cagcacccag gccgcgctga agccgaacag cacgccgccg ggtcgctcgc gcagccagcg
     1201 cgcccatgcc cagaaggtga acgcggggaa cagcgccagg cccaggcaga acagcagcgt
     1261 cagcacgccg gccagcggca ccggggccga gccgtactgg tgaatgctca catacaccca
     1321 ggacgccccg gtgccgaaca tgccgacgcc gaagctccac gcgcgcagcg ccgcggcgtc
     1381 cggacggagc cggcgcatca acgccagcag cgcgagcggg cacagcaccg ccaggggcca
     1441 gatgtcgaag ggcgcgagcg cggcgggcag cagcgcgccc gccagcaggg cggcggcgtg
     1501 gcccgcgatg ccggggcctg ccagtcgtgc ggggaaactc atccgcccga ggcgccggga
     1561 tccaggttca gccgcagcag gtggatctgg cggctgtcgg cgttgagcac cttgaagtgg
     1621 aagccctcga tctcggcgat ctcgttgcgc cgcggcaggt ggccgaaggt ctgcatcacg
     1681 atgccgccga tggtgtcgaa ttcctcgtcg ctcagtttcg cgtcgaagta cttgttgaat
     1741 tcctcgatcg gcgtcagcgc cttgatcagg tagtcgttct cggacagctt cttgatgaag
     1801 gtgtcctgct gcttgtcgtg ctcgtcctcg atctccccga cgatctcctc gagcacgtcc
     1861 tcgatggtga ccagcccggc cacgccgccg tactcgtcga tcacgatcgc gatgtggttg
     1921 cggttctcgc ggaactcgcg cagcagcacg ttcaggcgct tgctctcggg gatgaacgtg
     1981 gccgggcgca gcaactgcgc gatgtcgatg ctgcccggac cattggcgaa cagctgcccc
     2041 agcatgtcct tggcgagcac gatgccgagc acgtcgtcgc tgctgtcgcc cgttaccggg
     2101 tagcgcgagt gcccggactc gatcacgcgg cgcaccactt ccccgggcgc atcgtcgctg
     2161 tgcaggcaca ccatctgcgg gcgcgggatc atcacgtcgc gcacctgcag gtgcgcgacc
     2221 tcgagcgctc cctgcatgat gccgtgggtc tcggtgtcga tcacgtcgtt ctgctcggcg
     2281 atcaccagga tgtcgagcag gtccttgcgg gtgcgcgggt cggaggagaa cacctgcgcg
     2341 attttctcga gccacgatcg ttcgtcgtgc tcgtctgcgt tgcgatcgtc actcatggcc
     2401 gttcagcttg ttcatgctct tggtagggat cggggaattt caggccggcg aggatgcgca
     2461 cctcgagcgc ctccatcacc gcggcatcgc gcgcgcgttc gtggtcgtgg cccagcaggt
     2521 gcagcgtgcc atgcaccagc atgtgcgccc agtgcgcgcg cgctgccttg tgttgctctg
     2581 ccgcctcgcg gttcaccacc ggcgcgcaga gcacgatgtc cccgagcagc ggcacgtcca
     2641 cgcccggtgg cagcccggcc gggaacgaca gcacgttggt gggatagtcc ttgtggcggt
     2701 agcgcgcgtt cagttcacgc atctcgggct cgtcgacgat gcgcaccgac agctctgcct
     2761 ccgcgtgctc cgcgccgagc gccgcgcgca cccagcggcg catgctgggc gccccgggta
     2821 tcgcgggatc cgccgatgcg cagtccacgt agacgcgccc tggcatctta ctgcggcgta
     2881 tggccgcggc gcggcgcggc gggctcggag gcgagccgct tctcgaaggc ctcgtaggcc
     2941 tcgacgatgc gctgcaccag cgggtggcgc accacgtcgc gcggggtgaa ccaggtgaag
     3001 ccgatgccct cgacgccctc gaggaatgtc atgacctgcg ccaggcccga ttgcgtgccg
     3061 cgcggcaggt cgacctgtgt gacgtcgccc gtgatcaccg ccttcgagcc gaagccgatg
     3121 cgggtcagga acatcttcat ctgctccggc gtcgtgttct ggctctcgtc gaggatcacg
     3181 aaggcgtggt tcagcgtgcg tccgcgcatg taggcgagcg ggcagacctc gatgacgtgg
     3241 cgctcgatca gcttgctcac ggtctcgaag ccgagcatct cgtagagcgc gtcatagagc
     3301 gggcgcagat acggatcgat tttctgcgcg aggtcgccgg gcaggaagcc gagcttctcg
     3361 ccggcctcca ccgcggggcg gaccagcagg atgcggcgca cctggtcgct ctccagcgcc
     3421 gccaccgcgc aggccaccgc gaggtaggtc ttgccggtcc cggcggggcc gatgccgaag
     3481 ttgatgtcgt gctggtggat cgagcgcacg tagtgctgct gactcttgcc gcgcggcttg
     3541 atgaccgcgc ggcgcaggcg cacgacgccc agcggggcga cttcgccctc gccggcggct
     3601 tcggccgcgg tcagttcctc gacgccggat tgctgcaggc gcaggtgcac ctggtccggc
     3661 gtgagttcga ggtggcgggc cgtctcgtcg tagaggtgga tgatcaccgc gcgcgcggca
     3721 atccgggccg gttccgcgcc ctccacgacg aagtggttgc cgcggttgcg gatgctgacg
     3781 ccgaggcgtt cctcgatgag gtgcaggtgc tgatcgaact ggccgcacaa ccgcgccagg
     3841 cggcgggaat cgctgggttc gagtacgaac tgtagtgtct gcgggtcgtt caaaggggtt
     3901 tctggggcct cttgcggcca gggggacggg ccaaggataa cgtcaatcgg agacccgcga
     3961 aagcgcagcg ccctctactc gggccgccat tcatggcgac aaccgcgtgt ccgtgggtcg
     4021 ccattcatgg cgacagcagc gcccggcaag gcacccgtcg gcatgaatgc cgacctacag
     4081 cacctccgcg cgcagcgaat tcggcagcgc ctcggtgatc tccacgtcca cgaactgccc
     4141 gatcaggccc gtgtcatcgc tcctgaaatg ggtgagccgg ttgttctccg tgcgcccctg
     4201 caggcgcccc gggtcgcgct tggacacctc cgtgacgagc aggcgctgcg tgccgccgac
     4261 catgcgccgg ctgatctcga aggcctgctg gttgatgcgc gcctgcagca gcgccaggcg
     4321 ctgcttcttg agctcctcgg gcgtatcgtc gggcagatcg gcggccggcg tgccggggcg
     4381 cgcgctgtag acgaagctga acgagtggtc gaagcccacg tccgcgatca ggcgcatggt
     4441 gtcctcgaaa tccctttccg tctcgccggg gaagccgacg atgaaatccg aggaaatgct
     4501 gatatccggg cgcacgcggc gcagcttgcg gatggtcgac ttgtactcga gcgcggtgtg
     4561 gttgcgcttc atcgccgcga ggacgcggtc cgagccgctc tgcaccggga ggtgcacgtg
     4621 gctcaccagt tgcgggatgt cggcgtaggc ctgcaccagc gcgtcgctga actccagcgg
     4681 gtgcgaggtg gtgtagcgga tgcgcccgat gccctccacc gccgccacca ggtg
//
LOCUS       DAORUQ010000221         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_22824_length_4690_cov_6.823517, whole genome shotgun sequence.
ACCESSION   DAORUQ010000221 DAORUQ010000000
VERSION     DAORUQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22824_length_4690_cov_6.823517"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..2718
                     /locus_tag="PLW13_14315"
     CDS             4..2718
                     /locus_tag="PLW13_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQY71606.1"
                     /translation="MKPDSTHQDEPAATTAWHACSTAEVVDAADARPASGLDQEQVAQ
                     RLARYGRNELPAPPRRPPWLRFLLQFHNPLIYVLLVAGVVTLLLEDYIDAGVIIGVVL
                     VNALIGFIQEGKAEQALEAVKAMLANRATVLRDGERHEIDAALLVPGDIVLLESGTRV
                     PADLRLLREKNLRINEAALTGESVPVEKGTAAVAADAAVADRSCMAYAGTVVSFGQAR
                     GLVVATGQGTEIGRIGALVGEVQSLVTPLTRRLDQFARQITGFIIALGLVTFLFGYFL
                     RDMPVLEIFLAVVGLVVAAIPEGLPAIVTIVLAIGTRTMARNRAIVRRLPAVETLGSV
                     TVICSDKTGTLTRNEMTATSVMLPGQTLGVSGVGYAPEGGFHCGDEAIDPARCEPLQV
                     LAQCALLCNDARLLHDPDAGWLLAGDPTEGALLSLALKAGIDPQDVTAKPRIDEIPFE
                     SEHRFMATLHHDHEGHAFILLKGAPERVLELCVEEAGGERPDAAAWEARMHEAANDGQ
                     RVLALARCALPAGTTAIAMSDITPRFALLGLVGLIDPPREEAVAAVAECHRAGLRVKM
                     ITGDHGITAAAIGRQLGLEAENALSGELIEMLDDAALAQRAESTDVFARASPEHKLRL
                     VAALQARGELVAMTGDGVNDAPALKAADIGVAMGRKGTDAAREASDLVLTDDNFATIT
                     RAVREGRVVFDNIKKSLLFILPTNGGEAGVILLAVFAGLALPVTAGQILWVNMVTTVT
                     LALALAFESAEPGVMDRPPRPPTEALVTPLLAARIAYVSLLMMAVTFTVFEWELARGA
                     SIETARTAAVNMLVVGELVYLFNVRRFTASAFTRDILSGNKVALWMGILLLGMQALFT
                     YAPPLQKAFQTTALDGSSWLLIALLGAAKFLAVEAEKAVLRRMRVHWM"
     gene            2756..3526
                     /locus_tag="PLW13_14320"
     CDS             2756..3526
                     /locus_tag="PLW13_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71607.1"
                     /translation="MTRCPKCGHARTRAESHVHEGICPACGIAYAKWQARRGGSARPA
                     APVPASPGQAAPEEDDHGPLLARLRQQLLEVPERVEPAAFWGRAALLAALALWSVKFI
                     VNGIDWESIGGSFLHGANLAFHEFGHLFFRPFGEFMGILGGSLFQVLLPLALLAVFAV
                     RQRDNFAAAVALWWCGQNFVDLAPYIRDAEYRVLPLVGGGGEESHDWGNLLSMLDAVD
                     SCYALARTSFTLGALVMLAALAWGAYLLVLQRAQQRAQ"
     gene            complement(3561..4604)
                     /locus_tag="PLW13_14325"
     CDS             complement(3561..4604)
                     /locus_tag="PLW13_14325"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY71608.1"
                     /translation="MHEIVQDYYGKTLQATADLKTTACCDISAVPAWLKPLLGRIHPE
                     VLSRYYGCGLVCPPLLEGCRVLDLGCGSGRDVYALAQLVGPSGEVVGIDMTAEQLAVA
                     ARHRAWHAEAFGYANVCLLQGYIERLEELGLEPGSFDVIVSNCVVNLSPDKDAVLRGV
                     HRLLKEGGEFYFSDVYADRRVPASVRNDPVLYGECLGGALYWNDFLRLATRHGFHDPR
                     LVEDRPLAVTDPALALRTGNLRFFSATYRLFRIAALESACEDFGQAVVYRGSIPEQPH
                     RFQLDKHHDIETGRVFPVCGNTWRMLAETRFAPHFDFIGDFSRHCGLFAGCGGALPFD
                     TEPDATTVSGSCC"
BASE COUNT          758 a         1582 c         1638 g          712 t
ORIGIN      
        1 gcgatgaagc ccgacagcac ccatcaggac gagccggcag cgacgactgc atggcacgca
       61 tgctcgacgg cggaggtcgt ggatgcggcc gatgcacggc ccgcatcggg cctcgatcag
      121 gaacaggtcg cgcagcgcct cgcgcgctac gggcgcaatg agctgccggc accgccgcgg
      181 cgcccaccgt ggctgcgctt cctgctgcag ttccacaacc cgctgatcta cgtgctgctc
      241 gtcgccggcg tggtgacgct gctgctcgag gactacatcg atgcaggcgt gatcatcggc
      301 gtggtgctgg tgaatgcgct gatcggtttc atccaggaag gcaaggccga gcaggcgctg
      361 gaggcggtga aggccatgct ggccaaccgc gccacggtgc tgcgcgacgg cgagcgccac
      421 gagatcgacg ccgcgctgct ggtgcccggg gacatcgtac tgctggagtc gggcacgcgc
      481 gtgccggcgg acctgcggct gctgcgcgag aagaacctca ggatcaacga agcggcgctg
      541 accggcgaat cggtgcccgt ggaaaagggc actgccgcag tcgccgcgga cgccgccgtc
      601 gccgaccgtt cctgcatggc gtacgcgggc accgtggtga gcttcggcca ggcacgcggg
      661 ctggtcgtcg cgacgggaca agggaccgag atcggccgca tcggcgcgct ggtcggcgag
      721 gtgcagtcgc tggtcacgcc gctgacgcgc cggctcgacc agttcgcgcg ccagatcacc
      781 ggtttcatca tcgcgctcgg cctggtcacc ttcctgttcg gctattttct gcgcgacatg
      841 ccggtgctcg agatcttcct cgcggtggtc gggctggtgg tggcggccat ccccgaggga
      901 ctgcccgcga tagtcaccat cgtgctggcc atcggcacgc gcacgatggc gcgaaatcgc
      961 gcgatcgtgc gccgcctgcc ggccgtggaa acgctgggtt cggtgaccgt gatctgctcg
     1021 gacaagaccg gcacgctgac gcgcaacgaa atgaccgcta cgagcgtgat gctgcccggc
     1081 cagacgctcg gcgtgagcgg cgtcggctac gcgccggagg gcggcttcca ctgcggcgac
     1141 gaggcgatag accctgcccg atgcgagccc ctgcaggtgc tcgcgcagtg cgcgttactc
     1201 tgcaacgatg cccgtctact gcacgacccc gacgcgggct ggctcctcgc tggcgatccg
     1261 accgagggcg cgctgctttc gctggcgctc aaggccggaa tcgacccgca ggatgtgaca
     1321 gcaaagccgc ggatcgacga gattccgttc gagtccgagc atcgattcat ggcgacgctg
     1381 caccacgacc acgaggggca cgccttcatc ctgctgaagg gagcgcccga gcgcgtgctg
     1441 gagctttgcg tggaagaagc cggcggcgag cgacccgacg ccgcggcctg ggaagcacgc
     1501 atgcacgagg cggccaatga cggccagcgc gtactggccc tcgcacgctg cgccctgccc
     1561 gcaggcacca cggcgatcgc gatgagcgat atcacgccgc gcttcgcgct gctcggcctg
     1621 gtcgggctga tcgacccgcc gcgcgaggag gcagtagccg cggtggcgga gtgccatcgc
     1681 gcaggactgc gcgtgaagat gatcaccggc gaccacggaa tcaccgcggc ggcgatcggc
     1741 cgccaactgg gactggaggc cgagaacgcg ctgagcggcg aactcatcga gatgctcgac
     1801 gacgcagccc tggcgcagcg cgccgagtcg accgatgtgt tcgcgcgcgc cagccccgag
     1861 cacaagctgc gcctggtggc cgcactgcag gcgcgcggcg aactggtggc catgaccggc
     1921 gatggcgtga atgacgcgcc cgctctgaag gcggcggaca tcggcgtggc catggggcgg
     1981 aagggaaccg acgccgcgcg cgaggcctcc gacctggtgt tgaccgacga caattttgcg
     2041 accatcaccc gcgccgtgcg cgagggccgc gtggtgttcg acaacatcaa gaagtcgctg
     2101 ctcttcatac tgcccacgaa tggcggcgag gccggcgtga tcctgctcgc ggtcttcgcc
     2161 ggcctggccc tgccggtgac ggccggtcag atcctgtggg tgaacatggt caccaccgtc
     2221 acgctggcgc tggctctggc cttcgagtcc gcagagccgg gcgtcatgga ccggcccccg
     2281 cgccccccga ccgaagcact ggtcacaccg ctgctggccg cgcgcatcgc ctacgtcagc
     2341 ctgctgatga tggcggtgac cttcaccgtc ttcgaatggg aactcgcgcg cggcgccagc
     2401 atcgagaccg cccggacggc ggccgtgaac atgctcgtgg tgggcgaact ggtttacctg
     2461 ttcaatgtcc gccgtttcac cgcgtccgcg tttacacgcg acatcctctc cggcaacaag
     2521 gtggcgctgt ggatgggcat cctgctgctc ggcatgcagg cactgttcac ctatgccccg
     2581 ccgctgcaga aggcattcca gaccaccgcg ctcgacggca gctcgtggct gctgatcgcg
     2641 ctgctgggcg cggcgaagtt cctcgcggtg gaggccgaga aggccgtgct ccggcgcatg
     2701 cgagtgcact ggatgtgaac ggtccgcttc gccccggacc gggaatacgc ggccgatgac
     2761 gcgctgcccg aaatgcggtc acgcgcgcac gcgcgccgag agtcacgtgc acgagggcat
     2821 ctgccccgca tgcggcatcg cctacgccaa gtggcaggca cgccggggtg gcagcgcgcg
     2881 ccccgcggcg ccagtgccgg catcccccgg acaggcagca cccgaagaag acgaccacgg
     2941 cccgttgctc gcgcgcctgc gccaacaact gctcgaggtg ccggagaggg tcgaaccggc
     3001 cgcgttctgg ggccgcgcgg cgctgctggc cgcgctcgcg ctgtggagcg tgaagttcat
     3061 cgtgaacggc atcgactggg agagcatcgg cggctcgttc ctgcacggcg cgaacctcgc
     3121 cttccacgag ttcggccacc tgttcttccg tccgttcggc gagttcatgg gcatcctcgg
     3181 cggcagcctg ttccaggtgc tgctgccgct ggcgctgctc gcggtgttcg ccgtgcgcca
     3241 gcgcgataac ttcgcggcgg cggtcgcgtt gtggtggtgc gggcagaatt tcgtggacct
     3301 ggcgccctac atccgcgacg ccgagtaccg cgtgctgccg ctggtgggcg gcggcggtga
     3361 ggaaagccac gactggggca acctgctctc gatgctcgat gccgtcgaca gctgctacgc
     3421 gctggcgcgc acgagcttca cgctgggcgc gctggtgatg ctcgcggcac tggcgtgggg
     3481 cgcgtacctg ctggtgctgc aacgcgcgca acagcgggct cagtaggtcc ggctgaagcg
     3541 gcacctaccg caacgcacga tcagcagcaa ctgccggaca ccgtcgtcgc atccggttcc
     3601 gtatcgaagg gcagcgcacc gccgcagccg gcgaacagcc cgcagtggcg ggagaaatcg
     3661 ccgatgaaat cgaaatgcgg cgcgaagcgc gtctcggcga gcatgcgcca ggtgttgccg
     3721 cacaccggaa acacgcgccc cgtctcgatg tcgtggtgct tgtcgagctg gaaacggtgc
     3781 ggctgctcgg gaatgctgcc gcggtaaacc acggcctgac cgaagtcctc gcacgccgat
     3841 tccagcgccg cgatgcggaa cagccggtag gtcgcggaaa agaagcgcag gttgccggtg
     3901 cgcagggcga gcgcgggatc ggtgaccgcg agcgggcgat cctcgacgag gcgcgggtcg
     3961 tgaaagccgt gacgcgtcgc gagacgcagg aagtcgttcc agtacagcgc gcctccgagg
     4021 cactcgccat acagcaccgg atcgttgcgc acagacgccg gcacgcgccg gtcggcgtag
     4081 acatcggaaa aatagaactc gccgccctcc ttcaacaggc ggtgaacgcc gcgcagcacc
     4141 gcgtccttgt ccggcgagag gttcaccacg cagttcgaca cgatcacatc gaagctgccg
     4201 ggttcgagac cgagttcctc gagccgctcg atgtagccct gcaataaaca cacattggcg
     4261 tagccgaagg cctcggcgtg ccaggcgcgg tggcgcgcgg caaccgccag ttgctccgca
     4321 gtcatatcga tgccgaccac ttcgcccgat gggcccacga gctgggccag cgcatagacg
     4381 tcgcgccccg agccgcaacc gaggtcgagc acacggcagc cctccagcag cggcggacac
     4441 accagcccgc agccgtagta gcgcgacagc acctcggggt ggatgcgccc gagcaggggt
     4501 ttcagccacg ccggcaccgc gctgatgtcg cagcaggcgg tggtcttgag gtctgcggta
     4561 gcctgcaggg tcttgccgta atagtcctgg acgatctcgt gcatctcgac acctcctgac
     4621 tgtggatcgg cattctgtgg gttgatggga tggtcccctc cctccttacc ggcatcgagt
     4681 gccagactgg
//
LOCUS       DAORUQ010000222         4683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_22876_length_4683_cov_5.536085, whole genome shotgun sequence.
ACCESSION   DAORUQ010000222 DAORUQ010000000
VERSION     DAORUQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_4683_cov_5.536085"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLW13_14330"
     CDS             complement(<1..239)
                     /locus_tag="PLW13_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71609.1"
                     /translation="MRETEHAGRTVVVTGAGGGIGRAAAMLFAQQGAAVVCGDIDLAQ
                     ASETARRIAAAGGRALALAVDVASEASNAALVARA"
     gene            complement(282..2291)
                     /locus_tag="PLW13_14335"
     CDS             complement(282..2291)
                     /locus_tag="PLW13_14335"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY71610.1"
                     /translation="MSAPLLSVSELSTHIDSENGLVRALDRVSFDVSAGETFALLGES
                     GCGKSVTALSLMRLLPPGGFVAAGSVVLDGQDLCALSEAQMRQVRGAGMAMIFQEPGT
                     SLNAVLTVGQQIGEVLALHRGLKGAAADREAVALMTQVGIPDPQRRLGEYPFQLSGGM
                     KQRVMIAMALAGEPRLLIADEPTTALDVTIQAQVLDVMAALQRARGMAMILITHDLGV
                     VAKMADRVGVMYAGHLIEEAPRDRFFGAPAHPYSRKLFEALPEGHRERGVLATIPGSV
                     PPLTTRFTGCRFAPRCDRAWERCRAEAPSWHALGDGQRVRCHLHDPAESVRAAVEPVA
                     PRATGPEVRATQEVVLGVRDMKVHFPITRGVFRRVVGHVKAVDGVSLDIGAGRTLALV
                     GESGCGKTTVGKAILRLIEPSGGSARLGDTDIGSLSGAALRAARASMQMIFQDPFGSL
                     NPRLRVIEIIEEGMNALGVSGSAVERRARVAQIMEQVGLRPELAERYPHEFSGGQRQR
                     IAIARALAVSPRLIVCDEPTSALDVSVQAQILNLLQELQARLGVAYLFITHNIAVVEY
                     LAHEVAVMYLGRIVEFGSVAQVLGNPQHPYTRALLAAVPRASLAAGVAADAVLVKGDL
                     PSPSNPPAGCHFHPRCPLATAMCRQEYPHPRTVDGTHVVSCHLVG"
     gene            complement(2288..3745)
                     /locus_tag="PLW13_14340"
     CDS             complement(2288..3745)
                     /locus_tag="PLW13_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71611.1"
                     /translation="MNGFQVVILWSDALLAVLLLACCAGIAAALRREHLRRAWRAVFA
                     NRSAMAALVVLLAYLLVALLDSLHFRPELPRQEGAADAPAQYSVEVLSVLDVALARMR
                     TAREDSYSAPFATHAFQKRMIEMPDGTQRRDYPRLQFGGAHLVDPAADRGADIARRGL
                     GGAAAGIALWIVMAGALAALVARARREPVRRTLGAILRGDTPIAWNASGWMLLLVLCV
                     GGALFALSGAYHVFGTDATGNDVLYQTFKAMRIALLIGTLATFVVLPLGIGLGLAAGY
                     FGGWVDDLIQYVYTVISSIPYVLLVAASALMMQLVIEQHAQIFDTAAARADARLVSLC
                     VIIGAISWTTLCRLLRAETLKLRETDYVQAARCFGVSGVRIMLRHILPNAFHIVLITV
                     VLEFSGLVLAEAVLSYVGVGVDPTTISFGTMINNARGELAQDPVIWWSITAAFVFMVS
                     LVLAANLFADAVREAFDPRGAVPRRRRRVAQESAA"
     gene            complement(3742..4683)
                     /locus_tag="PLW13_14345"
     CDS             complement(3742..4683)
                     /locus_tag="PLW13_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71612.1"
                     /translation="MPTLIGVNLLLFTLFFVISSPDDMARAQLGAKRVTPEAIERWKA
                     DRGYDKPLFWNAQASGAERFTETIFVQKSARMFLFDFGKSDVGRDINLELRTRMWPSL
                     ALALPVFLVGLFVAITLALLLAFLRATWLDFTGVIICVVLMSISSLFYIIAGQFLVAR
                     VWNLVPISGFATGLDAARFLALPVAVGVLAGLGSNVRWYRAIFLEEIGKDYVRTARAK
                     GLSEVAVLFRHVLKNGMIPILTGTVVVIPSLFLGSLITESFFGIPGLGTFTIEAILMQ
                     DFAIVRSMVFIGSVLYIAGLILTDLSYTLVDPRVRLQ"
BASE COUNT          813 a         1694 c         1567 g          609 t
ORIGIN      
        1 tcggcgcgcg cgaccagcgc ggcgttcgac gcttcgctcg ccacgtccac cgcgagcgcc
       61 agcgcgcggc ccccggcagc cgcgatccgg cgcgcggttt cgcttgcctg cgcgaggtcg
      121 atatcgccgc agaccaccgc cgcgccctgc tgcgcgaaca gcatcgcggc ggcacggccg
      181 atgccgccac cggcgcccgt caccacgacc gtcctgccgg catgctccgt ttcgcgcatc
      241 ctcgatcctc ccgtcgacaa ccgttgtggc gccgcgatgg ctcaacccac gagatgacaa
      301 ctcacgacat gcgtgccgtc caccgtgcgc ggatgcggat actcctggcg gcacatcgct
      361 gtggcgagcg ggcagcgcgg gtggaagtgg caaccggcgg gcgggttgga cggcgacggc
      421 aggtcgcctt tcaccagcac ggcatcggcc gcgacgccag cagccaggct ggcgcgcggc
      481 accgcggcca gcagcgcgcg ggtatagggg tgctgcggat tgccgagcac ctgcgcgacg
      541 ctgccgaatt cgacgatgcg ccccaggtac atcaccgcga cctcgtgcgc gaggtattcc
      601 accaccgcga tgttgtgcgt gatgaacagg taggccacac cgaggcgcgc ctgcagttcc
      661 tgcaacaggt tgaggatctg cgcctgcacc gagacgtcta gcgcggaggt gggttcgtcg
      721 cagacgatca gccgcggcga cacggccagc gcgcgcgcta tcgcgatgcg ctggcgctgt
      781 ccgccggaga attcgtgcgg gtagcgttcg gccagctccg gcctcagccc cacctgctcc
      841 atgatctgcg caaccctggc gcggcgctcg acggcgctgc cgctcacgcc gagcgcgttc
      901 atcccttcct cgatgatctc gatcacgcgc aggcgcggat tcagcgagcc gaacggatcc
      961 tggaagatca tctgcatgct ggcgcgcgcc gcgcgcagcg ccgcccccga caggctgccg
     1021 atatcggtgt cgccgagccg cgcgctgccg ccgctgggct cgatcagacg caatatcgcc
     1081 ttgccgaccg tggtcttgcc gcaaccggac tcaccgacca gcgccagtgt gcgtccggcc
     1141 ccgatgtcga ggctcacgcc gtccaccgcc ttcacgtgac cgacgacgcg ccggaacacg
     1201 ccgcgggtga tcgggaaatg caccttcatg tcgcgcacgc cgagcacgac ctcctgcgtc
     1261 gcgcgcacct cggggcccgt agcgcgcggc gcaaccggct ccacggcggc gcgcaccgac
     1321 tcggcgggat cgtgcaggtg acagcgcacg cgctgcccgt caccgagcgc atgccaggac
     1381 ggcgcctcgg cgcgacaacg ctcccacgcg cgatcgcagc gcggcgcgaa gcggcagccc
     1441 gtgaaccgcg tggtcagcgg cggcacgctg cccggaatcg tggccagcac gccgcgctcg
     1501 cggtgcccct cgggcagggc ttcgaacagc ttgcgcgaat acggatgggc cggcgcgcca
     1561 aagaagcgat cgcgcggcgc ctcctcgatc aggtggcccg cgtacatcac gccgacgcgg
     1621 tcggccatct tcgcgaccac gccgagatcg tgggtgatca ggatcatcgc catgccgcgc
     1681 gcgcgctgca gcgcggccat cacatcgagc acctgcgcct ggatggtgac gtcgagcgcc
     1741 gtggtcggct cgtcggcgat cagcagccgc ggctcccccg cgagcgccat cgcgatcatg
     1801 acgcgctgct tcatgccgcc ggagagctgg aagggatact cgccgaggcg ccgctgcggg
     1861 tccgggatgc cgacctgcgt catcagcgcc acggcctcgc ggtccgccgc cgcgcccttc
     1921 aggccgcgat gcagcgccag cacctcgccg atctgctggc ccaccgtcag caccgcgttc
     1981 aggctggtgc cgggctcctg gaagatcatc gccatgccgg cgccgcgcac ctggcgcatc
     2041 tgcgcctcgc tcagcgcgca cagatcctgt ccgtccagca ccacgctgcc cgcggcgacg
     2101 aagcccccgg gtggcagcag gcgcatcagc gacagcgcag tgaccgattt gccgcaaccg
     2161 gactccccga gcagcgcgaa ggtctcgccc gcgctgacgt cgaacgacac gcgatccagc
     2221 gcgcgcacga gcccgttctc gctgtcgata tgcgtcgaca gctccgatac cgacagcagc
     2281 ggagcgctca tgccgcgctc tcctgcgcca cgcggcgacg gcggcgcggc acggcgccgc
     2341 gcgggtcgaa ggcctcgcgc accgcgtcgg cgaacaggtt ggccgccagc accaggctca
     2401 ccatgaacac gaaggcggcg gtgatggacc accagatgac cgggtcctgc gcgagctccc
     2461 cgcgcgcgtt gttgatcatg gtgccgaagc tgatcgtggt cgggtcgaca ccgacgccca
     2521 catacgacag cacggcctcg gcgagcacca gcccggagaa ctccagcacc accgtgatca
     2581 gcacgatgtg aaaggcgttc ggcaggatgt ggcgcagcat gatgcgcacg cccgagaccc
     2641 cgaagcagcg cgccgcctgc acgtagtcgg tctcgcgcag cttgagcgtc tcggcgcgca
     2701 acagccggca cagcgtggtc caggatatcg cgccgatgat cacgcacagc gacaccaggc
     2761 gcgcgtcggc gcgcgcggcg gcggtgtcga agatctgcgc gtgctgctcg atcaccagct
     2821 gcatcatcag ggccgaggcc gcgaccagca gcacgtaggg tatcgagctg atcaccgtgt
     2881 acacatactg gatcaggtca tcgacccagc cgccgaaata gcccgccgcc aggcccagcc
     2941 cgataccgag cggcagcacc acgaaggtgg ccagcgtgcc tatcagcagc gcgatgcgca
     3001 tcgccttgaa cgtctggtac agcacgtcgt tgccggtggc gtcggtgccg aacacgtggt
     3061 aggcgccgga gagcgcgaac agcgcgccgc ccacgcagag caccaacagc agcatccagc
     3121 ccgatgcgtt ccaggcgatc ggcgtatcgc cgcgcagtat ggcgcccagg gtgcggcgca
     3181 ccggctcgcg tcgtgcgcgc gccaccagcg ccgcgagcgc acccgccatg acgatccaca
     3241 gcgcgatgcc ggccgccgca ccaccgagcc cgcgccgggc gatatcggca ccgcgatccg
     3301 ccgcgggatc caccaggtgc gcaccgccga actgcagccg cggatagtcg cgccgttgcg
     3361 tcccgtcggg catctcgatc atccgcttct ggaatgcgtg cgtggcgaac ggcgcggaat
     3421 agctgtcctc gcgcgccgtg cgcatccgcg ccagcgccac gtcgagcacc gacagcactt
     3481 ccaccgagta ctgcgccggc gcatccgcag ccccctcctg gcgcggcagc tcggggcgaa
     3541 agtgaaggga gtccagcagc gcgaccagca ggtaggcgag cagcacgacg agggcggcca
     3601 tcgccgaacg gttcgcgaat accgcgcgcc acgcgcggcg caggtgctcg cggcgcagcg
     3661 ccgccgcgat cccggcgcag cacgcgagca gcagcacggc gaggagcgcg tcggaccaga
     3721 ggatcacgac ctggaagccg ttcattgcag gcgcactcgc gggtcgacca gggtgtagga
     3781 gagatcggtg agtatcagtc ccgcgatgta gagaaccgag ccgatgaaga ccatcgagcg
     3841 cacgatcgcg aaatcctgca tcaggatcgc ctcgatcgtg aaggtcccga gaccgggaat
     3901 gccgaagaag gactccgtga tcaggctgcc gaggaacagc gagggaatca ccaccaccgt
     3961 cccggtgagg atcgggatca tgccgttctt cagcacgtga cggaacaata ccgcgacctc
     4021 ggagagcccc ttggcgcgcg cggtacgcac atagtccttg ccgatctcct ccaggaagat
     4081 cgcgcgatac cagcgcacgt tcgagccgag cccggcaagc accccgactg ccaccggcag
     4141 cgcgaggaaa cgcgccgcgt ccagccccgt cgcgaaaccc gagatgggca cgaggttcca
     4201 cacgcgcgcg accaggaatt gcccggcgat gatgtagaac aggctcgata tggacatcag
     4261 cacgacgcag atgatcacgc cggtgaaatc gagccaggtg gcgcgcagga aggccagcag
     4321 caacgccaga gtgatcgcca cgaacaggcc caccaggaac accggcagcg cgagcgccag
     4381 cgagggccac atgcgcgtgc gcagctcgag gttgatgtcg cgcccgacgt cggacttgcc
     4441 gaagtcgaac aggaacatgc gcgcggattt ctgcacgaag atcgtctcgg tgaagcgctc
     4501 ggcgccgctc gcctgcgcgt tccagaacag gggcttgtcg tagccgcgat cggccttcca
     4561 gcgctcgatg gcctccggcg tcacgcgctt ggcaccgagc tgcgcacggg ccatgtcgtc
     4621 gggactgctg atcacgaaga acagcgtgaa cagcagcagg ttcacgccga tcagcgtggg
     4681 cac
//
LOCUS       DAORUQ010000223         4647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_23150_length_4647_cov_5.849956, whole genome shotgun sequence.
ACCESSION   DAORUQ010000223 DAORUQ010000000
VERSION     DAORUQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23150_length_4647_cov_5.849956"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLW13_14350"
     CDS             <1..208
                     /locus_tag="PLW13_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71613.1"
                     /translation="TAWPAPPAEEIASIRAESMLRAPREPGLLQKGEGGARCAGDSEK
                     RSAAGRSRRGAHRVITCRYRGMPS"
     gene            205..1239
                     /locus_tag="PLW13_14355"
     CDS             205..1239
                     /locus_tag="PLW13_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71614.1"
                     /translation="MSRWNREEIDTCFRRFMDITDRAFIEGDLEPWVQCFTEDAVYSD
                     LGYGFNGGWDSEIRGRDAIRAWINGHFASFPNNQMAFWPVPWYVIDAQRGWVICEWRN
                     RMRDPGNGMVLEEKNYSRLKYAGNMQWSFEEDIYNPLRMRTMIELWRHERARCEAAGL
                     QMPEPRPEQALAMHQAVREHDVNPGWSRTEIEDTLKRFEDAGNRAFQTGDHSEWLQFY
                     TPDVQYREHGFGFAGGWEHEIHGRTAVKQWIDPHCDAWPINRMVDFPIEWHVIDEARG
                     WVLLEYRNIMADPGDGTRLETRSYTRLKYGGNGQWRFEEDIYSPLRMRAMLDAWVLLN
                     SRAGGSRGAG"
     gene            1335..2429
                     /locus_tag="PLW13_14360"
     CDS             1335..2429
                     /locus_tag="PLW13_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HQY71615.1"
                     /translation="MYPRLFSPLQLGAIELKNRIVMAAMTRARSPSTVPNAANATYYA
                     QRAGAGLIITEATQVSIQGRGAWATPGIHTPEQIAGWRRVTDAVHAAGGKIFCQLQHS
                     GRVSHTFYQEDGRPPVSSSAVKGAVKTFIDTGFVDTPAPRALGIEEIPGVVEQYRAGA
                     LNAIAAGFDGVEIHGANGYLVEQFLRDGVNRRADAYGGTVENRCRFLLEVTDAVCAAV
                     GAQRTAVRLSPFSVTWDCSDSDPKPLYAHAVAQLATRDLAFLEVVEAINEAGDISSGG
                     ASNAFNITHILAAYNGRLVINGGYDAVRAEAVLARPGIDAVSFARPFICNPDLAERFA
                     RGAPLTPMADPAVIYGGGEAGYIDFARMAG"
     gene            complement(2467..3711)
                     /locus_tag="PLW13_14365"
     CDS             complement(2467..3711)
                     /locus_tag="PLW13_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQY71616.1"
                     /translation="MSASAPFRPRHMKLSVLTAALQELTPRERRDPDPDLAIEEWLEY
                     AREIGAPNIQLSAALHPDVSDVPAAALLDPVANTLDLREPFDARRAARVRAAMRASAV
                     GLSDLAYFDNMLAADAGLRRSKHEFMLRVFDAAAMLGVDGVCGFVGRNPQLQMDANLV
                     MFEEVFVPLLAAAKARGLTYRAEQCPMPGWTVNDAWHNNIAYAPGPWIALHRICEKHG
                     VGDQFRIHYDPSHAILMGQDTRSVFQYLKDAGYAFLVAGFHVKGQVIDPRGLAAWGYG
                     GQTLQRGDWHGAIPADDPAAQSSAWKKQTVFSQHELPGTARHDPLGYLQNRTVDWLDH
                     QLAARELLPLDPANTYLVVEHEYPPARVQDRALLAPLLAGSIAFVKAIDEAAAAMYAL
                     QHELLPRQGIPVQGVGREAYRS"
     gene            complement(3708..>4647)
                     /locus_tag="PLW13_14370"
     CDS             complement(3708..>4647)
                     /locus_tag="PLW13_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY71617.1"
                     /translation="LAAKTRLAEGERIEVLAVLTPNHLHYPMVLRALESGLHVICEKP
                     FTMTASEALELERRARQGGRKVCIAHTYTGYPMVRQMRAMIAAGEIGSVQKIDAQYYQ
                     GWINAAMHDAAMRQSIWRLDPARSGPSCCYGDIGVHVFNLAEYTTGLRIARVLADLDT
                     FRPDNALDVDGTTLLRTACGVHGVLRASQIATGEENGVQIAVYGSRGALKWSHETPAT
                     LTHLREDAPRQVFTPGQRFNAELARTASQLPPGHPQGLAEAMANLYRGFADAIHGVPG
                     AEERFPGIEAGVRGMRFIEATLASSRAGQTWMEISA"
BASE COUNT          744 a         1631 c         1565 g          707 t
ORIGIN      
        1 gacggcgtgg ccggcgcctc cggccgagga gatcgcgagc atcagggcgg agtcgatgct
       61 gcgcgcgccg cgcgagcccg gcctcctgca aaagggtgag gggggcgcgc gctgtgcagg
      121 cgacagtgaa aagcgtagtg cagccggccg atcccggcgc ggcgcgcacc gcgtgattac
      181 ttgtcgatac cgggggatgc cgtcatgagc cgatggaaca gggaagagat cgacacctgc
      241 tttcgccgct tcatggatat cacggaccgg gcgttcatcg agggggacct ggaaccctgg
      301 gtgcaatgct tcaccgagga cgccgtctac agcgacctcg gctacggctt caacggcggc
      361 tgggacagcg agattcgcgg ccgggatgcg atccgtgcct ggatcaatgg gcacttcgcg
      421 agtttcccca acaaccagat ggccttctgg cccgtgccct ggtacgtgat cgacgcgcag
      481 cgcggctggg tgatctgcga gtggcgcaac cgcatgcgcg accccggcaa cggcatggtc
      541 ttggaggaga agaactacag ccgcctgaag tacgccggca acatgcagtg gagcttcgag
      601 gaggacatct acaacccgct gcgcatgcgc acgatgatcg agctctggcg ccacgagcgc
      661 gcgcgctgcg aggcagccgg cttgcagatg cccgagcccc ggccggagca ggcactcgcg
      721 atgcaccagg ccgtacgcga gcacgacgtg aacccgggct ggtcacgcac cgagatcgag
      781 gataccctga agcgcttcga ggacgccggc aaccgcgcct tccagacggg tgatcacagc
      841 gagtggctgc agttctacac cccggacgtg cagtaccgcg agcacggctt cggcttcgcg
      901 ggcgggtggg agcacgagat ccacggtcgc accgcggtca agcagtggat cgacccgcac
      961 tgcgacgcct ggccgatcaa ccgcatggtc gacttcccga tcgaatggca cgtgatcgac
     1021 gaggcgcgcg gctgggtgct gctggagtac cgcaacatca tggccgaccc gggggacggc
     1081 acccgcctcg agacgcgcag ctacacgcgg ctcaagtacg gcggcaacgg gcagtggcgc
     1141 ttcgaggagg acatctacag cccgctgcgc atgcgcgcca tgctggacgc ctgggtgctg
     1201 ctgaattcgc gggcgggagg atcgcgcggc gccgggtaga gccgtctgcg ccggcgctcc
     1261 ccgtcgggca gcctcgtcca accaccgcgt gttcccttgc ttcgatcaaa tcccccacac
     1321 aggaatcagg caccgtgtac cccagactct tctccccctt gcaactcggc gcgatcgagc
     1381 tgaagaaccg catcgtcatg gccgcgatga cgcgtgcgcg ctcaccgtcc acggtaccca
     1441 acgcggccaa cgcaacctac tacgcgcagc gcgcgggcgc cggcctgatc atcaccgagg
     1501 ccacgcaggt ctcgatccag ggccgcggcg cctgggcgac gcccggcatc catacgccgg
     1561 agcagatcgc gggttggcgg cgggtcacgg acgccgtgca cgccgccggc gggaagatct
     1621 tctgccagct ccagcactcg ggccgcgtgt cgcacacctt ctaccaggag gacgggcgac
     1681 cgcccgtttc ctcctcggcg gtcaagggag cggtgaagac cttcatcgac accggcttcg
     1741 tagatacgcc cgcgccgcgt gcactcggca tcgaggagat ccccggcgtc gtcgagcagt
     1801 atcgcgccgg tgcgctcaat gcgatcgccg cgggcttcga cggcgtcgag atccacggcg
     1861 cgaacggcta cctggtcgaa cagttcctgc gcgacggcgt gaaccgccgc gccgacgcct
     1921 acggtggcac cgtcgagaac cgctgccgct tcctgctcga ggtcaccgac gcggtctgcg
     1981 cggcggtcgg cgcgcagcgc accgcggtgc ggctctcgcc gttctccgtg acctgggact
     2041 gcagcgacag cgaccccaaa ccgctctacg cgcacgccgt cgcgcaactc gccacgcgcg
     2101 acctcgcgtt cctcgaggtg gtcgaagcca tcaacgaggc cggtgacatc agctccgggg
     2161 gcgcgagcaa cgccttcaac atcacgcaca tcctggcggc gtacaacgga cggctggtca
     2221 tcaacggcgg ctatgacgcc gtgcgcgccg aggcggtgct cgcgcggccg ggcatcgacg
     2281 ccgtctcctt cgcccggccg ttcatctgca accccgatct cgcggagcgc ttcgcgcgcg
     2341 gcgcgccgct cacgccgatg gccgatccgg cggtgatcta cggcggcggc gaggcgggct
     2401 acatcgactt tgcgcgcatg gcgggctgac ccggcggcga cgccatcgaa cgctggagag
     2461 ccccgctcag gatcggtagg cctccctgcc cacaccctgt accgggattc cctggcgcgg
     2521 gagcagctcg tgctgcagcg cgtacatcgc ggcagcggcc tcgtcgatcg ccttgacgaa
     2581 ggcgatcgaa ccggcgagca gcggcgcgag cagcgcgcga tcctggaccc tcgccggcgg
     2641 atactcgtgc tcgaccacga ggtacgtgtt cgccggatcc agcggcagca gttcgcgcgc
     2701 cgcgagctgg tgatccagcc agtcgacggt ccggttctgc agatacccca gcggatcgtg
     2761 gcgcgcggta ccgggcagct cgtgctggct gaacacggtc tgcttcttcc acgcgctgga
     2821 ctgcgcggcg ggatcatcgg cgggaatcgc gccgtgccag tcgccgcgct gcagggtctg
     2881 cccgccgtag ccccaggcgg cgaggccgcg cggatctatc acctgcccct tcacatggaa
     2941 ccccgctacc aggaaggcgt agcccgcgtc cttcaggtac tggaagaccg agcgcgtatc
     3001 ctggcccatg aggatcgcgt gcgaggggtc gtaatggatg cggaactgat cgccaacgcc
     3061 atgtttctcg cagatccggt gcagcgcgat ccacggaccc ggagcgtagg cgatgttgtt
     3121 gtgccaggcg tcgttcaccg tccagccggg catcgggcac tgctcggccc ggtaggtgag
     3181 tccgcgcgcc ttcgctgccg cgagcagtgg cacgaagact tcctcgaaca tcaccagatt
     3241 ggcgtccatc tgcagttgcg gattgcgccc gacgaagcca cagacgccat ccacgccgag
     3301 cattgccgcg gcgtcgaaca cgcgcagcat gaactcgtgc ttgctccggc gcagccctgc
     3361 gtccgcggcc agcatgttgt cgaagtacgc gagatccgag agcccgaccg cgctggcgcg
     3421 catcgccgcg cgcacgcgtg ccgctcgccg cgcgtcgaag ggctcccgca ggtcgagcgt
     3481 attggcgacc ggatcgagca gcgccgcggc gggcacgtcg ctgacgtcgg gatgcaacgc
     3541 cgcggagagc tgtatgttcg gcgcgccgat ctcgcgcgcg tactccagcc actcctcgat
     3601 ggcgagatcc ggatccgggt cgcgccgctc gcgcggcgtg agttcctgca gtgccgccgt
     3661 gagcacgctg agcttcatgt gccggggacg gaacggcgcg ctcgcgctca tgcgctgatc
     3721 tccatccagg tctggcccgc gcgactggac gccagcgtcg cttcgatgaa gcgcatgccc
     3781 cggactccgg cttcgatgcc gggaaagcgc tcctccgcac ccggcacgcc gtggatcgca
     3841 tcggcaaagc cgcggtagag attggccatg gcctcggcca gcccctgcgg atgtccgggc
     3901 gggagctggc tcgcagtgcg cgccagctcc gcattgaagc gttggcctgg cgtgaacacc
     3961 tgccgcggcg cgtcttcgcg cagatgcgtc agggtcgccg gcgtctcgtg actccatttc
     4021 aatgcgccgc ggctgccata gacggcgatc tgcacgccgt tctcctcgcc cgtggcgatc
     4081 tggctcgcgc gcagcactcc atgcacgccg caggccgtgc gcaacagcgt cgtgccgtcc
     4141 acgtccagcg cattgtcggg acggaacgtg tcgagatccg ccagcacgcg cgcaatgcgc
     4201 aagcccgtgg tgtactccgc gaggttgaag acatgcacgc cgatgtcgcc gtagcagcaa
     4261 ctcggccccg atcgcgccgg atccagccgc cagatcgact ggcgcatcgc cgcatcgtgc
     4321 atggccgcgt tgatccagcc ctggtagtac tgcgcgtcga tcttctgcac cgaaccgatc
     4381 tcgccggccg cgatcatcgc gcgcatctgc cgcaccatcg ggtagcccgt gtaggtgtga
     4441 gcgatgcaca ccttgcgccc gccctgccgc gcgcgccgct ccagctcgag cgcctcgctc
     4501 gcggtcatcg tgaacggctt ctcgcagatc acgtgcagcc ccgactccag ggcccgcagc
     4561 accatcgggt agtgcaggtg attcggcgtc agtaccgcga gcacctcgat gcgctcaccc
     4621 tcggcgagtc gtgtcttggc cgcgagc
//
LOCUS       DAORUQ010000224         4603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_23515_length_4603_cov_5.406552, whole genome shotgun sequence.
ACCESSION   DAORUQ010000224 DAORUQ010000000
VERSION     DAORUQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23515_length_4603_cov_5.406552"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLW13_14375"
     CDS             complement(<1..478)
                     /locus_tag="PLW13_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQY71618.1"
                     /translation="MKALAKLKAEPGIWMTDVPEPAVGHNDVKIRIRKTAICGTDTHI
                     FRWDQWSQDTIRVPMVIGHEYVGEIVEIGQEVSGFRIGDRVTGEGHITCGYCRNCRAG
                     RRHLCRNTIGVGVNRPGAFAEYLVIPAFNAFKLSEEISDDLAAIFDPFGNAVHTALS"
     gene            complement(571..1623)
                     /locus_tag="PLW13_14380"
     CDS             complement(571..1623)
                     /locus_tag="PLW13_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQY71619.1"
                     /translation="MKAAVFQGVGLIETRTDLARPRPGPGEVLIQVASCGICGSDLHI
                     YRLDSPSAREMYAGTLRVDADGKRIIGHEYAGVIAEIGPGVEGYAPGDRVVGVTGGGG
                     MAEYVPVPANPFQLARIPAGVSFEEAATTEPLADSLQLVRKAAIAAGENVVVFGVGII
                     GLGVIQALRAVAPQVGRIVAVDVSASRLAKALEVGASDVIDAAAEDVVEAAGRACGVL
                     RTEYPRMDTPDVAVVIDCAGYIKHMKGAPPLQTALDLLRPHGGRIVCFGAFEDEVTLK
                     LTNLIHKQPLIMGSLGYLAEELTQALALMAAGTVDRRALISDRFPLARADEAFRTQGN
                     GRAIKVMVQPHPQMND"
     gene            complement(1715..3841)
                     /locus_tag="PLW13_14385"
     CDS             complement(1715..3841)
                     /locus_tag="PLW13_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY71620.1"
                     /translation="MSAYEHLMSPGRIGAMELRNRIFMTPMGSNTADDEGYCGERLRQ
                     YYGARAEGGAALLIMGSVAISWPVGSANWRQVAISDDRFIPGLQSVADVVHGHGAKLA
                     VQLQHAGLTAMNDIRDGRPMLTPSVPKEKEGDMGPYLFPEEAAAMSTPYANPHAKVAY
                     QVATEADIAWLIGQFADAADRARRAGIDAVELHAGHGYVIHSFLNPSVNQRSDRWGGS
                     LENRARLLVEVIRAIKQRCGTDYPVWCRLDGIEFLTEGGITHEDAKDTARMAEAAGAD
                     AIHVSSYADASKGESFTAAHTTHKPCGFVEYAAGIKSVVNIPVITAGRIEPEVGERLI
                     RQGRIDFVTMGRKLLADPELPRKINEGRAHEIRPCIYCYTCISQIFVNRSVICAVNAR
                     TGREFETVISPAEQPKRILIAGAGPAGMEAARVARLRGHTVILCDKASRLGGTVFFST
                     ASYPPNGGLVRYLSGEMERLGVEVRLNTEVTPELIAREKPDAVVAAVGARRESPALPG
                     IELPHVLNGDDIRAMITGEGGEGGGPKLGPVVGTLLKLGKLVGILGRPDWMRELSRVW
                     MPMGKRVVMIGGGLVAIELAEFLAERGRKVTILEEGKVFGAGLMLVRRWRNISDCRKL
                     GVEMLAEATLVRIDAGSLVYRNANGQERRIAADQVIITSGATANPGPAERMRSAGVPV
                     HEIGDCAGMAYIDGSVRAGFDLAMRL"
     gene            complement(3873..>4603)
                     /locus_tag="PLW13_14390"
     CDS             complement(3873..>4603)
                     /locus_tag="PLW13_14390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71621.1"
                     /translation="VCLVTGAASGIGRATALRLAAEGASIAAGDINEAGLQSLVVELT
                     QMGAPCMVIPFNALEEASNRALVHRAVEQFGRLDVLCNIAGACRADHFLDIRLGDWER
                     MFAINVTSLVAICQEALPHLCETRGNIVNMSSASALCGAAYWSAYAASKAAVASLSKC
                     LAVEFSGKGVRVNAVCPGGVNTTLATTYSVPPDVDMALVQRLFPLFPAAEPGEIASAV
                     AYLASDEARYVTGVAFNIDGGQMT"
BASE COUNT          700 a         1640 c         1492 g          771 t
ORIGIN      
        1 aggacagcgc cgtgtgcacc gcgttgccga agggatcgaa tatcgcggcg agatcatcgc
       61 tgatctcctc gctcagcttg aaggcattga atgcggggat cacgaggtat tcggcgaagg
      121 cgccggggcg gttcacgccg acaccgatcg tgttgcggca caggtggcgc cgcccggcgc
      181 ggcaattgcg gcagtagcca caggtgatgt ggccctcgcc ggtgacgcgg tcgccgatgc
      241 gaaaaccgct cacttcctgt ccgatctcga cgatctcgcc gacgtattcg tgcccgatca
      301 ccatcggcac ccggatggtg tcctgcgacc actggtccca gcggaagatg tgcgtgtcgg
      361 tgccgcagat cgcggtcttg cggatgcgga tcttgacgtc gttgtgcccg accgcgggct
      421 ccggcacatc ggtcatccag atgccgggtt cggctttgag cttggccagt gctttcacgc
      481 agcgggtctc ggtgggcggc gcgcagatga tacccgcaag cgccgtcgcg gcgtaggtcg
      541 ggcttcactc cgacccggtg cttgcaacgc tcaatcgttc atttgcggat gcggctgaac
      601 catcaccttg atcgcgcgtc cgttgccctg cgtgcgaaat gcctcgtccg cccgcgccag
      661 cgggaagcgg tccgagatca gtgcgcggcg gtctaccgtg cctgcggcca tcagcgcgag
      721 cgcctgcgtc agttcctcgg ccaggtagcc cagcgagccc atgatcagcg gctgcttgtg
      781 gatgaggttt gtcagcttca gcgtgacttc gtcctcgaag gcgccgaagc acacgatccg
      841 cccgccgtgc gggcgcagca gatccagcgc cgtctgcagc ggcggggcgc ccttcatgtg
      901 cttgatgtag ccggcgcagt cgatcacgac cgcgacgtcc ggcgtgtcca tgcgcggata
      961 ttcggtgcgc agcaccccgc aggcccgccc ggccgcttcc accacgtcct cggcggctgc
     1021 atcgatcacg tcgctggcgc cgacttccag cgccttcgca agccgcgacg ccgacacgtc
     1081 caccgcgacg atgcgtccga cctgcggcgc caccgcacgc agcgcctgga tcacgccgag
     1141 cccgatgatg cccacgccga acaccacgac gttctcgccc gcggcaatag ccgccttgcg
     1201 caccagttgc aggctgtcgg ccagcggctc cgtggtcgcg gcctcctcga aggagacgcc
     1261 cgcgggaatg cgcgcgagct gaaacggatt tgccggcacc ggcacgtact cggccatgcc
     1321 gccgccaccg gtaacaccca ccacgcgatc gcccggcgca tagccctcga cccccgggcc
     1381 gatctccgcg atcacgcccg cgtattcgtg gccgatgatg cgcttcccgt ctgcatcgac
     1441 acgcagcgtg cccgcgtaca tctcgcgcgc cgacggcgag tcgaggcggt agatgtgcag
     1501 atcgctgccg cagatgccgc aggaggcgac ctgtatcagt acctcgccgg ggcccggccg
     1561 cggtcgcgcc aggtccgtgc gggtctcgat gaggccgaca ccctgaaaaa ccgctgcctt
     1621 catgccgggc tcctcgattt gtgggtccga attcattcgg acgattgtcc cgtgggtccg
     1681 gatttgcccg gacatcgtcc ggctgaagcc ggacctacaa ccgcatcgcc agatcgaagc
     1741 ccgcgcgcac cgagccgtcg atatacgcca tgcccgcgca atcgccgatc tcgtgcaccg
     1801 gcacgccggc actgcgcatg cgctcggcgg gcccgggatt cgccgtcgca cccgaggtga
     1861 tgatcacctg gtccgcggcg atgcgccgtt cctggccgtt ggcgttgcgg taaaccagac
     1921 tcccggcatc gattcgcacg agcgtggcct cggccagcat ctccacgccg agcttgcggc
     1981 aatcggagat gttgcgccag cgccgcacca gcatcaggcc ggcgccgaag accttgcctt
     2041 cctcgaggat cgtgaccttg cgcccgcgct cggcgaggaa ctcggccagt tcgatggcga
     2101 ccagcccgcc gccgatcatc accacgcgtt tgcccatcgg catccagacg cggctcaact
     2161 cgcgcatcca gtcgggcctg cccaggatgc cgacgagctt gccgagtttc agcagcgtgc
     2221 cgaccacggg gccgagcttc ggcccgccgc cctcgccccc ttccccggtg atcatcgcgc
     2281 ggatgtcgtc gccgttcagt acatgcggca gctcgatgcc cggcagcgcc ggcgattcgc
     2341 ggcgcgcgcc cacggcggcc acgaccgcat cgggtttctc gcgcgcgatc aactccggcg
     2401 tgacctcggt gttcagccgc acctcgacgc cgagccgctc catctcgccg ctgaggtagc
     2461 gcacgaggcc gccgttgggc ggatacgagg cggtgctgaa gaacaccgtg ccgcccaggc
     2521 gcgaggcctt gtcacacaaa atcacggtat gcccgcgcag tcgcgcgacc cgcgcggcct
     2581 ccatgccggc cgggccggcg ccggcgatca ggatgcgttt cggctgctcg gccggcgaga
     2641 tcacggtctc gaactcgcgc ccggtgcgtg cgttgaccgc gcagatcacg ctgcggttca
     2701 cgaagatctg gctgatgcag gtgtagcagt agatgcaggg ccggatctcg tgcgcgcgcc
     2761 cttcgttgat cttgcgcggc agttccgggt ccgcgaggag cttgcgcccc atggtcacga
     2821 agtcgatcct cccctggcgg atcaagcgct cacccacctc gggttcgatg cgcccggccg
     2881 ttatcacggg aatgttcacc accgacttga tgccggccgc gtactccacg aagccgcacg
     2941 gcttgtgcgt ggtgtgcgcc gcggtgaagc tctcgccctt cgaggcatcg gcgtaggagc
     3001 tcacgtggat cgcgtcggcg ccggccgcct cggccatgcg cgccgtatcc ttcgcgtcct
     3061 cgtgcgtgat gccaccctcg gtcaggaact cgatgccgtc gaggcggcac cacaccgggt
     3121 agtccgtgcc gcaacgttgc ttgatcgccc tgatcacctc gaccagaagg cgagcgcggt
     3181 tctccagcga gccgccccag cgatcgctgc gctggttcac cgaggggttc aggaagctgt
     3241 ggatcacgta gccgtgtccc gcatgcagtt ccaccgcgtc gatgcccgcg cgcctggcgc
     3301 gatccgcggc atcggcgaac tggccgatca gccacgcgat gtccgcctcc gtcgccacct
     3361 ggtaggcgac cttcgcatgc ggattggcat agggcgtgga catcgcggcc gcttcctcgg
     3421 ggaacaggta gggccccatg tcgccctcct tttccttcgg caccgagggc gtcagcatcg
     3481 gccggccgtc gcggatgtcg ttcatcgcgg tgagtcccgc gtgctgcaat tgcaccgcga
     3541 gttttgcgcc atgcccgtgc accacgtccg cgaccgactg cagccctgga atgaagcggt
     3601 cgtcggatat cgcgacctgg cgccagttcg ccgaacccac cggccagctg atcgccaccg
     3661 aacccatgat cagcagcgcc gcgccgcctt cggcgcgcgc gccgtagtac tggcgcaggc
     3721 gttcaccgca gtagccctcg tcatcggcgg tgttggagcc catcggggtc atgaagatgc
     3781 ggttgcgcag ttccatggcg ccgatgcggc ccggggacat caggtgttcg tatgcgctca
     3841 tatgctggtg atccgtgttg tttcgtgcgt gctcaggtca tctgcccgcc gtcgatattg
     3901 aatgccacgc cggtgacgta gcgcgcctcg tcgctggcga gataggccac ggccgatgca
     3961 atctccccgg gttccgccgc cggaaacagc gggaacaggc gctgcaccag cgccatgtcc
     4021 acatcgggcg gcacgctgta ggtcgtggcc agcgtggtgt tcacgccacc cgggcagacc
     4081 gcgttgacgc gcacgccctt gccggaaaat tccactgcga gacacttgct cagcgacgcc
     4141 accgccgcct tcgaggccgc atacgcgctc cagtaggccg ccccgcacag cgccgaggcc
     4201 gaggacatgt tcacgatgtt gccgcgcgtc tcgcacagat gcggcagcgc ctcctggcag
     4261 atcgcgacca ggctggtgac attgatcgcg aacatgcgct cccagtcgcc gagcctgatg
     4321 tcgaggaaat gatcggcgcg gcaggcaccg gcgatgttgc acaggacgtc gaggcggccg
     4381 aactgctcca ccgcgcgatg cacgagcgcg cggttcgaag cctcctcgag cgcgttgaac
     4441 gggatcacca tgcacggcgc gcccatctgc gtgagctcga cgacaaggct ctgcaggccc
     4501 gcctcgttga tgtcgcccgc ggcgatggac gccccctccg cggccaggcg cagcgcggtg
     4561 gcgcgcccga tgcccgacgc cgcacccgtg accaggcaaa cct
//
LOCUS       DAORUQ010000225         4587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_23637_length_4587_cov_5.234996, whole genome shotgun sequence.
ACCESSION   DAORUQ010000225 DAORUQ010000000
VERSION     DAORUQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23637_length_4587_cov_5.234996"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PLW13_14395"
     CDS             <1..1461
                     /locus_tag="PLW13_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HQY71622.1"
                     /translation="ETHMNGFAQVAALVAPWTPEKQAEVTGVPADTLRELVAAYLAAD
                     GAALYGSTGINQGSNGSTAFWLLEVINAVTGNLDRRGGALMGRGIVDYAKATANADAK
                     VFRSRIGNTPSFLGALPTALLADEILVPGPDQVRAMFVISGNPLLTGTNSARMAQAFG
                     ELELRVSIELVRNETAELADYILPGSHFAERPDLPFSFFTFSGLMPEPWIQYTGRMVA
                     PPGEARDESWILARLAAGCGAPLFGSRLLQAVLDAGELARRLPFVGPRLKALPDRVLD
                     LILRVANLGGTRGLRKFPHGKPLPRNGGNNYLGQRVLTASGKVELAPAALLELAAVRL
                     PRSHEQALGTRDEFRLITRRERYTHNSWAHNDAAFVKGRRHTNYLYMHPADAARLGIA
                     DGTTARVESAAGALEVPVALSADLMPGAVALPHGWGHQSATGLSVASKTRGVNANLLA
                     ADGPDAIEPLSGMAQFNGIAVRISAVAAGAAARAAT"
     gene            1547..2152
                     /locus_tag="PLW13_14400"
     CDS             1547..2152
                     /locus_tag="PLW13_14400"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQY71623.1"
                     /translation="MDPLLPPAFLPAYFLVFFALAFAWRSLLVWWRTGINPVVLPAGD
                     DAHAYVGRAFKIVMAACGAVAAAPVLCADAPRWLGAWEALAAVPVAACGWLLLLAALA
                     WVVVAQAQMGAAWRIGIDNARPTALVQHGLFARSRNPIFLALRVELLGFFLVLPGAAT
                     AALLVAGEILMQVQVRLEEQHLAALHGADYADYCARVRRWL"
     gene            2181..2810
                     /locus_tag="PLW13_14405"
     CDS             2181..2810
                     /locus_tag="PLW13_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQY71624.1"
                     /translation="MKLYHCRDSRSLRPLWALEEMGIPYELEDLPFPPRLFRKEYLGI
                     NPLGTVPYFVDGETRMTESVAICHYLVERYHRHELGLLPEHPEYGSYLNWLYHSDATL
                     TFPQTIVLRYRHLEPPERQLAQAVQDYRGFFLGRLKLLDAHIATREWLCDGRFTIADI
                     CIGYALYLGSLPLIDAADGYSPQVSAYLARLMARPAFQRAAALGAPLFG"
     gene            2940..4568
                     /locus_tag="PLW13_14410"
     CDS             2940..4568
                     /locus_tag="PLW13_14410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY71625.1"
                     /translation="MSASMLIFAPSFAGTVLGIGVAVIAGMRRSGPAWPYMVLLGIAV
                     GWWCLGQTFWILADTPAEALRVNQVQYVGVQFAPLLWLLVALAQTGHRRWLQPWRVAP
                     LLVVPLLTLAFAFSYRLDQPNWLWSGFHVDVGSVVPSASYGPWFKVFVIYNYALFLTG
                     CVLLYGHYVQSPHYRLQLWVTSLVPALLLGMNVSFVAGYWPLPLDPTPLGFALGFALL
                     GWALLRHQLLDLRPLGRSIAFDSLGQGVLIVDAEERIVDLNAAAGRLLRRSPATVLGR
                     PLAQMLPGLDLGAVSAGGRELAIAAGDYAELRLQVGMAPIVVERGAPQGMVLTLQDVT
                     REREAQQTLLGMQRRLEEANRELERVAHTDMLTGLANRRLLLARLEMEFSRARRHGTP
                     LALLLIDLDRFKQVNDTRGHLVGDAVLRTTGAALRELTRPEDIPARYGGEELALLLTD
                     TGADGARTAALRVWQHLRSIEHEDPAAGSTFRVSCCIGVALLDLTDSHANDLIARADA
                     ALYAAKADGRDCVMLSLNGVLEREPPPVIESAVA"
BASE COUNT          585 a         1674 c         1616 g          712 t
ORIGIN      
        1 gagacgcaca tgaacggctt cgcgcaggtc gcggcgctcg tcgcgccttg gacgccggag
       61 aagcaggccg aggtcaccgg cgtgccggcc gatacgctgc gcgagctggt ggcggcctac
      121 ctcgcggccg atggcgcggc gctctatggc tccaccggca tcaaccaggg cagcaacggc
      181 agcaccgcgt tctggctgct cgaggtcatc aacgccgtca ccggcaacct cgatcggcgc
      241 ggcggcgcgc tgatgggccg gggcatcgtc gattacgcga aagccaccgc caacgccgac
      301 gcgaaggtgt tccgctcgcg catcggcaac acaccgagct ttctcggcgc gctgcccacc
      361 gcgctgctgg ccgacgagat cctcgtgccc ggacccgacc aggtgcgcgc gatgttcgtg
      421 atctccggca acccgctgct gaccggcacc aacagcgcgc gcatggcgca ggccttcggc
      481 gaactggagc tgcgcgtcag catcgagctg gtgcgcaacg agacggcgga gctcgcggac
      541 tacatcctgc ccggctcgca cttcgccgag cggcccgacc tgccgttctc gttcttcacg
      601 ttttccggcc tgatgcccga gccgtggatc cagtacaccg ggcgcatggt ggcgccgccc
      661 ggcgaggcgc gcgatgagag ctggatcctg gcgcggctgg cggcgggctg cggcgcgccg
      721 ttgttcggct cgcgcctgct gcaggccgtg ctcgacgccg gcgagctggc gcggcgcctg
      781 cccttcgtgg ggccgcggct gaaggcgctg cccgatcgcg tgctcgatct catcctgcgt
      841 gtcgcgaatc tcggcggcac gcgcgggctg cgcaaattcc cgcacggcaa gccgctgccg
      901 cgcaacggcg gcaacaacta cctcggccag cgcgtgctca ccgcgagcgg caaggtggag
      961 ctggcgccgg cggcgctgct ggaactcgcc gcggtgcgcc tgccgcgcag ccacgagcag
     1021 gcgcttggca cgcgcgatga attccggctg atcacgcggc gcgagcgcta cacccacaat
     1081 tcctgggcgc acaacgacgc ggccttcgtg aagggccggc gccacaccaa ttacctgtac
     1141 atgcaccccg ccgacgcggc gcggctgggt atcgccgacg gcacgacggc gcgcgtggag
     1201 tccgcggccg gcgcgctcga ggtcccggtc gcgctcagcg ccgacctgat gcccggcgcc
     1261 gtcgcgctgc cgcacggctg gggtcaccag tcggccacgg gcctgtcggt ggcctcgaag
     1321 acgcgcggcg tcaacgcgaa cctcctggcg gcggacggcc ccgatgccat cgagccgctg
     1381 tcgggcatgg cgcagttcaa cggcattgcc gtgcgcatca gcgctgtcgc cgccggggcg
     1441 gcggctcgcg ctgccacctg aggacggtgc gagcccgcgg gcttgatcgc gcaccgcccg
     1501 caccgtagcc tgcggccatc gactgctgcc ggggacacag acgcccatgg accccctgct
     1561 tccgccggcg ttcctgccgg cctatttcct ggtcttcttc gcgctcgcct ttgcgtggcg
     1621 atcgctgctg gtgtggtggc gcaccggcat caaccccgtg gtgctgcccg cgggcgacga
     1681 tgcgcacgcc tacgtcgggc gcgcgttcaa gatcgtgatg gccgcctgtg gcgcggtggc
     1741 ggccgcaccg gtgctctgcg cggatgcgcc gcgctggctg ggcgcctggg aggcgctcgc
     1801 cgcggtgccg gtggcggcct gcggctggtt gctgctgctg gcggcgctgg cgtgggtggt
     1861 ggtcgcgcag gcgcagatgg gggccgcgtg gcgcatcggc atcgacaacg cgcgccccac
     1921 cgcgctggtg cagcacggcc tgttcgcccg ctcgcgcaac ccgatcttcc tcgcgctgcg
     1981 cgtggagttg ctcggcttct tcctggtgct gccgggcgcc gccaccgcgg cgctgctggt
     2041 cgccggcgag atcctgatgc aggtgcaggt acggctcgag gagcagcacc tggcggcact
     2101 gcacggcgcg gactacgcgg actactgcgc gcgcgtgcgg cgctggttat gatccgccgg
     2161 acaaccgggg aagcatcggc atgaagctct atcactgtcg cgattcgcgc tcgctgcgcc
     2221 cgctgtgggc gctggaggag atgggcatcc cctacgagct cgaggacctg cccttcccgc
     2281 cgcgcctgtt ccgcaaggag tacctcggca tcaacccgct gggaacggtg ccgtacttcg
     2341 tcgacggcga gacgcgcatg accgagtccg tcgcgatctg ccactacctg gtcgagcgct
     2401 accaccgcca tgagctgggg ctgctgcccg agcaccccga gtacggcagc taccttaact
     2461 ggctctacca cagcgatgcc acgctcacct tcccgcagac catcgtgctg cgctaccgcc
     2521 acctcgagcc gccggagcgc cagctcgcgc aggccgtcca ggactaccgc ggcttcttcc
     2581 tgggccggct caagctgctc gatgcgcaca tcgccacgcg cgagtggctc tgcgacggac
     2641 gcttcacgat cgccgacatc tgcatcggct atgcgctcta cctcggcagc ctgccgctga
     2701 tcgacgcggc cgacggttac tcgccgcaag tgagcgccta cctcgcgcgg ctcatggcac
     2761 gccccgcgtt ccagcgcgcc gcggccctgg gggcaccgtt gttcggctga cgagggcgtc
     2821 gcaaaaccct gcacgcgccg gtgtaggagc ggcccatggc cgcgatatcg cgcgcatggc
     2881 gcgctcctac actatatttg actgccaccc cgcacatcgc cggagcccgc catcccccca
     2941 tgtccgcgtc gatgctgata ttcgccccct cgttcgcggg caccgtgctc ggtatcggcg
     3001 tggcggttat cgcggggatg cggcgctcgg ggcccgcgtg gccctacatg gtgctgctcg
     3061 gcatcgccgt ggggtggtgg tgtctcgggc agaccttctg gatcctcgcc gacacgcccg
     3121 ccgaggcgtt gcgcgtgaac caggtgcagt acgtcggggt gcagttcgcc ccgctgctct
     3181 ggctgctggt cgcgctggcg cagaccgggc accggcgctg gctgcagccg tggcgcgtgg
     3241 cgccgctgct ggtggtgccc ctgctcacgc tggcgttcgc gttcagctac cgcctcgacc
     3301 agccgaactg gctgtggtcc ggattccacg tcgatgtcgg ctccgtggtg ccgagcgcaa
     3361 gctacgggcc ctggttcaag gtcttcgtga tctacaacta cgcgctgttc ctcaccggct
     3421 gcgtgctgct ctacggccac tacgtgcagt cgccgcacta ccggctgcag ctgtgggtga
     3481 ccagcctggt gccggcgctg ctgctcggca tgaacgtgag cttcgtcgcc ggctactggc
     3541 cgttgccgct cgaccccacc ccgctcggct tcgcgctcgg cttcgcgctg ctcggctggg
     3601 cgctgctgcg ccaccagctg ctcgacctgc gcccgctcgg gcgcagcatc gccttcgaca
     3661 gcctcgggca gggcgtgctg atcgtggatg ccgaggagcg catcgtcgac ctgaacgccg
     3721 ccgcgggccg gctgctgcgc cgcagcccgg cgaccgtgct cggccgcccg ctcgcccaga
     3781 tgctgccggg gctggacctc ggtgccgtgt ccgcaggcgg cagggagctc gcgatcgcgg
     3841 cgggcgacta cgccgagctg cggctgcagg tcggcatggc cccgatcgtg gtcgagcgcg
     3901 gcgccccgca gggcatggtg ctgacgctgc aggacgtcac gcgcgagcgc gaggcgcagc
     3961 agacgctgct cgggatgcag cgccggctcg aggaggccaa ccgcgagctc gaacgcgtgg
     4021 cgcacaccga catgctcacg gggctggcca accgccgcct gctgctggcg cgcctggaga
     4081 tggaattctc gcgcgcgcgc cgccacggca ccccgctcgc gctgctgctg atcgacctcg
     4141 accggttcaa gcaggtcaac gacacgcgcg ggcacctggt gggcgacgcg gtgctgcgca
     4201 ccacgggcgc ggcattgcgc gagctgacgc gccccgagga catcccggcg cgttacggcg
     4261 gcgaggagct ggcgctgctg ctgaccgaca ccggcgccga cggcgcgcgc accgcggcac
     4321 tgcgcgtgtg gcagcacctg cgctccatcg agcatgaaga tcccgccgcg ggcagcacct
     4381 tccgcgtgag ttgctgcatc ggcgtggcac tgctggatct gaccgattcc cacgccaacg
     4441 atctcatcgc gcgcgccgac gcggcgctct acgcggccaa ggccgacggg cgcgactgcg
     4501 tgatgctgag cctcaatggc gtgctggagc gcgagccgcc gccggtgatc gaatcggcgg
     4561 tggcctgagc agcgtccgtg gggccgc
//
LOCUS       DAORUQ010000226         4504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_24254_length_4504_cov_5.429759, whole genome shotgun sequence.
ACCESSION   DAORUQ010000226 DAORUQ010000000
VERSION     DAORUQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24254_length_4504_cov_5.429759"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..230
                     /locus_tag="PLW13_14415"
     tRNA            157..230
                     /locus_tag="PLW13_14415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:189..191,aa:Gly,seq:ccc)
     gene            complement(397..2118)
                     /locus_tag="PLW13_14420"
     CDS             complement(397..2118)
                     /locus_tag="PLW13_14420"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71626.1"
                     /translation="MGDYDLVIRGGTLLDGSGGEPYIADVGVRGGLIADVGPRLGRAR
                     EEIDAAGLLVTPGFVDIHTHYDGQATWENRLVPSSAHGVTTAIMGNCGVGFAPCRPEH
                     REPLIRLMEGVEDIPFPVLTQGLPWTWESFPEYLDLLASRRYDMDIACYLPHAALRVY
                     VMGERGANREPATPADIARMCELLGEALDAGAMGIATSRTIFHRSSDGRPIPTLEAGD
                     DELLALADTLRARGTGVFQIVEDMHEPDTSLAHMRRIAERAGRPLTFSIGTGNTGPWH
                     WPRLLEELGEANAAGITMKAQLLPRAIGMMLGHELTLNPFYSTATYAELARLPLAQRL
                     HELRSPEVRARILAEPPEADPALVLGRMVRDFDHMFLLGDPPDYEQPREQSITSRARR
                     LGMSAEELAYDLMLEGDGRGKLYLAMANYAEGKLDALGTMLRHPDVLPGLGDGGAHCA
                     TICDGSYSTFTLMHWGRDRAEGRLPLAQLVHGLARATALTMGLRDRGLLAPGYKADIN
                     VIDLERLALHPPELTPDLPGGGRRLLQRAEGYVATIVNGRTVYRDGQHSGELPGRLVR
                     GERARPG"
     gene            complement(2022..2375)
                     /locus_tag="PLW13_14425"
     CDS             complement(2022..2375)
                     /locus_tag="PLW13_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY71627.1"
                     /translation="MDEMAERDAEEGDALVAQLIAHALQPRFRHVHVRENDDIVLWDN
                     RRTMHCTFSHPVEQVRIVQCSTIRGTVAIGRILGDEREAARAWATTIWSFAAARCSTA
                     RAANRTSPTWGCAAG"
     gene            complement(2480..3019)
                     /locus_tag="PLW13_14430"
     CDS             complement(2480..3019)
                     /locus_tag="PLW13_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQY71628.1"
                     /translation="MKVRALFPATALCTLIALAGAPAAQAAGEKVSLVQKAIELNAEG
                     PYAGAFDTLIAAVLAADPAVVSTLSDNSQETIFAPTDDAFAALGLTSENIGSALDQAT
                     LTTVLLYHVAPGRRYAEDVVSAERVRTLLKGKKGFFQVDGTVLTDNLGRTSNIIATDV
                     EADNGVIHAIDNVLLPVAP"
     gene            3293..>4504
                     /locus_tag="PLW13_14435"
     CDS             3293..>4504
                     /locus_tag="PLW13_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HQY71629.1"
                     /translation="MRNKTTPLDLPPRVTIARTRPGESSPARPLIDPRAPILFAILAL
                     LLLASVARGATTTYEVLLDLDLNAQTGCTISTPKGSVGGIEQALVTTVVTDTGGGSVS
                     GVARRVCTAAVLGAPLPVDAGGWPVGFGNGTLGSTVIETYIPRAALGQATSAAAAVRV
                     TGDGGGGDAIVGPEGGSLPTVSFAPPGNGVPQMIPLLSPWALVLLALLLGVLASRTVR
                     RGDALGMLFAVVLGSAVSALVWAATVILDGNVGDWSGVQPSATDAMGNAPPGGDLVAL
                     FTQSDQQRVYFRIDADLRPEVPGVLLEGEADELALTALTSLPPVPADMDDIVNGVILS
                     RLDAAFDAEATVGQINAALSAINGRIAGMQPGQPFLVVALPRQADLAALDARAEDFQS
                     RPGILLALPGRE"
BASE COUNT          660 a         1524 c         1553 g          767 t
ORIGIN      
        1 agccaaaatc agtgacagag agggggtttt gcgacaccct cttcagccgg acaatgtcga
       61 catgaattcc gacctacggc cggacaccgg cgaggccctg cgtgaacccg ggctttgaat
      121 gcgcgccgcc gatggttaga atggcgccgt tcgctcgcgg gtgtagttca atggtagaac
      181 tgcagcttcc caagctgcta gcgtgggttc gattcccatc acccgctcca tatgcatagg
      241 ccaccatcaa ggtggccttt gtgtttggac agggcgcatg ggtgagaagt cacacgcatc
      301 cgacaaaccg gcaggagagc cggtctggac gcgcgagccg tgatgcggcg aacccctgga
      361 attccatttc gtcaggtccg ctacgccccg cgcgcctcaa cccggccgcg cccgttcccc
      421 ccgcaccagc cgtcccggca actcgccgct gtgttgcccg tcgcggtaga cagtgcgccc
      481 gttgacgatg gtcgcgacat agccttcggc acgctgcagc agacggcgcc cgccgccggg
      541 caggtcgggc gtcagttccg gcggatgcag cgcgagccgc tcgaggtcga tcacgttgat
      601 gtccgccttg tagcccggcg ccagcagacc gcggtcgcgc agtcccatcg tgagcgccgt
      661 ggcgcgggcg agtccgtgca ccagttgcgc cagcggcagg cgcccctcgg cgcggtcgcg
      721 gccccagtgc atcagcgtga acgtggagta gctgccgtcg cagatcgtgg cacagtgcgc
      781 gccgccgtcg cccagccccg gcagcacgtc gggatggcgc agcatggtgc ccagcgcatc
      841 gagcttgccc tcggcgtagt tggccatcgc aaggtagagc ttgccgcgtc cgtcgccttc
      901 cagcatcagg tcgtaggcga gttcctccgc actcatgccg agccggcgcg cgcgcgaggt
      961 gatgctctgc tcgcgcggtt gctcgtagtc gggtggatcg ccgagcagga acatgtggtc
     1021 gaagtcgcgc accatgcgtc ccagcaccag cgcgggatca gcctccggcg gttcggccag
     1081 gatgcgcgcg cgcacttcgg ggctgcgcag ttcgtgcaga cgctgcgcga gcggcaaccg
     1141 ggccagttcg gcgtaggtcg cggtggaata gaacgggttc agcgtcagct cgtggccgag
     1201 catcatgccg atcgcgcgcg gcagcagctg cgccttcatg gtgatcccgg cggcgttggc
     1261 ctcgcccagt tcctccagca gccgcggcca gtgccacgga ccggtgttgc cggtaccgat
     1321 cgagaaggtg agcgggcgcc ccgcgcgctc ggcgatgcgg cgcatgtgcg ccaggcttgt
     1381 gtcgggttcg tgcatgtcct ccacgatctg gaacacgccc gtgcccctgg cgcgcagcgt
     1441 gtcggccagc gccaggagtt cgtcgtcacc ggcttcgagc gtcgggatcg gtctgccgtc
     1501 gctggagcgg tggaagatcg tgcgcgaggt ggcgatgccc atcgcgccgg cgtcgagtgc
     1561 ctcgccgagc agctcgcaca tgcgtgcgat gtcggccggc gtggccggct cgcggttcgc
     1621 gccgcgttcg cccatcacgt agacgcgcag cgccgcgtgc ggcaggtagc aggcgatgtc
     1681 catgtcgtag cgacgtgagg cgagcaggtc caggtactcc gggaagcttt cccaggtcca
     1741 gggcaggccc tgcgtcagca ccggaaacgg gatgtcctcc acgccctcca tcaggcggat
     1801 cagcggctcg cggtgctcgg gccggcaggg cgcaaagccc acgccgcagt tgcccatgat
     1861 cgcggtggtc acgccgtgcg ccgaggacgg taccaggcgg ttctcccagg tcgcctggcc
     1921 gtcgtagtgg gtgtggatgt cgacgaagcc gggcgtgacc agcagccccg cggcgtcgat
     1981 ctcctcgcga gcgcgcccga gtcgcggccc cacgtcggcg atcagcccgc cgcgcacccc
     2041 cacgtcggcg atgtacggtt cgccgcccga gccgtcgagc agcgtgccgc cgcgaatgac
     2101 cagatcgtag tcgcccatgc ccgcgctgcc tcccgttcgt ccccgaggat cctgccgatc
     2161 gcgacagtac cgcggatcgt gctgcattgc acgatgcgca cctgctccac cggatggctg
     2221 aaggtgcagt gcatggtgcg ccggttgtcc cagagcacga tatcgtcgtt ctcccggacg
     2281 tggacgtgcc ggaagcgcgg ttgcagcgcg tgcgcgatca gctgcgcgac cagcgcgtcg
     2341 ccttcctccg cgtcccgctc cgccatctcg tccatgccgc gctgcgcagc gctccgttgc
     2401 cgttccttcg tcggatcggt tacggacaaa aaacgggggg ctgatgcccc cccgaggtcg
     2461 agaccatcaa tgtcgggctt cagggtgcaa ccggcagcag cacgttgtcg atcgcgtgga
     2521 tcacgccgtt atccgcttcc acatcggtcg cgatgatgtt gctggtgcgc cccaggttgt
     2581 cggtgagcac ggtgccatcg acctggaaga agcctttctt gcccttgaga agggtgcgca
     2641 cccgctcggc gctcacgacg tcctcggcgt aacggcgccc cggcgcaacg tggtacagca
     2701 gcaccgtggt gagagtggcc tggtcgagcg ccgagccgat gttctcgctc gtcaacccga
     2761 gcgcggcgaa cgcatcgtcg gtgggcgcga aaatggtctc ctggctgttg tcgctcaacg
     2821 tcgagaccac cgcggggtcc gccgcgagca cggccgcgat cagggtgtcg aaggcgccgg
     2881 catacggccc ttctgcattc agctcgattg ccttctgcac cagcgagact ttttccccgg
     2941 ccgcctgtgc tgccggcgcg ccggcaagag cgatgagcgt gcaaagagcc gtcgcgggga
     3001 acagtgcgcg tactttcatg cggaactcct cctgtgggtt ggctgggacc ggcgcaccac
     3061 cgcaaccgtg gggtggttcg cgctgtgtcc gaaccggata cgcgggtctg ccacgggccg
     3121 gatcaccggc gcgcgttcca gtttcccgtc gggacgaagc atcgggcagg gcccagtctc
     3181 gttattgcac gcgcaggatt ttcggcgcag catgcaatcg ccactgtggt acccctacgg
     3241 tgccgatgcg tccccgggta atggttgcag cacgttcaaa cgggagcggg ccatgcgcaa
     3301 taagaccaca ccccttgatc tcccgccgcg cgtgactatt gcgcgaacaa gaccgggcga
     3361 aagcagtccg gcgagaccac tgatcgatcc tcgcgccccg atactgtttg caatcctggc
     3421 gttgctgctg ctcgcgtccg tggctcgagg cgcaaccacg acttacgagg tactgctaga
     3481 cctcgacctg aacgcccaaa ccggttgcac gatttccacg cccaagggca gcgttggcgg
     3541 catagagcag gcgctggtca ccacggtggt cacggacacg ggcggcggat ccgtctcggg
     3601 cgtggcgcgc cgtgtctgca ccgcggcggt gctcggtgct ccgctgccag tggacgccgg
     3661 cggctggccc gtgggtttcg gcaacgggac gcttggctcg accgtgatcg aaacttacat
     3721 tccgcgggca gcgctcgggc aggcgacgag tgcggcagcg gcagtccggg tgaccggcga
     3781 tggcggcgga ggcgacgcca tcgtcgggcc ggagggcggt tcgctgccga cggtctcgtt
     3841 cgcgcccccg gggaacgggg ttccgcagat gattccgctg ctttccccgt gggccctggt
     3901 gttactggcg ttgctcctgg gcgtgctcgc ctcgcgtacc gtacgccgtg gcgatgccct
     3961 cgggatgctc ttcgccgtgg tgctgggctc agccgtcagt gcattggtct gggccgcgac
     4021 ggtgatcctc gatggcaatg tcggggactg gtccggggtc cagcccagcg cgacggatgc
     4081 catgggcaac gcaccgcccg gcggcgatct ggtcgcgctg ttcacccaga gcgaccagca
     4141 gcgggtgtat ttccgcatcg atgccgacct gcgtccggag gtgccgggcg tactgctgga
     4201 gggcgaggcg gacgagctcg cgttgacggc gctcacctcg ctgccgcctg ttccggccga
     4261 catggatgac atcgtcaacg gcgtcatcct gtcgcgcctg gacgccgcct tcgacgccga
     4321 agccacggtg ggacagatca atgccgcgct gtctgccatc aacgggcgaa ttgcagggat
     4381 gcagccggga cagccgttcc tcgtggtggc gctgccgcgc caggccgacc tcgccgcgct
     4441 ggacgcgcgc gccgaagact tccagtcccg ccccggcatc ctgctcgcgt tgcccggccg
     4501 cgag
//
LOCUS       DAORUQ010000227         4375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_25366_length_4375_cov_5.626157, whole genome shotgun sequence.
ACCESSION   DAORUQ010000227 DAORUQ010000000
VERSION     DAORUQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25366_length_4375_cov_5.626157"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..501
                     /locus_tag="PLW13_14440"
     CDS             118..501
                     /locus_tag="PLW13_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71630.1"
                     /translation="MYRFDPRQFDLSAWARWVASGRPVHFSMLGNSLCGQPFMPQHKR
                     TYRDDSPLWQEHDGEIDEHVARLMRIDSVVHGALSVSERRLLLAAVAPSGTGHPLPLV
                     ERARLAGASPEALRAIRRKAMALID"
     gene            complement(892..981)
                     /locus_tag="PLW13_14445"
     tRNA            complement(892..981)
                     /locus_tag="PLW13_14445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Ser,seq:cga)
     gene            complement(1082..1795)
                     /locus_tag="PLW13_14450"
     CDS             complement(1082..1795)
                     /locus_tag="PLW13_14450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQY71631.1"
                     /translation="MEKRDLIVKGKAKSMYTTDDAAYCIMHFRDDTSAFDGKRIEQLE
                     RKGRVNNIFNAFIMEKLEAAGIPTHFVKRIDDRDSLVRRMAMIPVECVVRNIAAGSIC
                     RRLGVKEGIDLDPPTFELFLKNDELGDPMINEYHIAAFKWANLKDVETARELTFKVNG
                     VLKKLFSDHGMLLVDYKLEFGVCDGKVLLGDEFTPDGCRIWDAETREKLDKDRFRQSL
                     GNVVESYEIVGRRLGLSFD"
     gene            complement(1826..2584)
                     /locus_tag="PLW13_14455"
     CDS             complement(1826..2584)
                     /locus_tag="PLW13_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY71632.1"
                     /translation="MQFCSLGSGSAGNALLVRTASTCVMVDCGFGRRELERRMAQRGM
                     HPRDLAGILVTHEHSDHAGGVAALARHYGIAVFASAGTAKAARLEGAKPVVLVPERPE
                     RIGDIEVLPVTVPHDAREPCQFVLGHARRSLGVLTDLGSVSKLVRDQYARCDALVLEC
                     NHDAEMLANGPYPYPLKRRVGGDFGHLSNAQAASLLAGVERSALQHLVAAHLSEQNNA
                     PAKARAALEEVLGQGDERLLVADQESGFGWLAIQ"
     gene            complement(2639..3769)
                     /gene="bamC"
                     /locus_tag="PLW13_14460"
     CDS             complement(2639..3769)
                     /gene="bamC"
                     /locus_tag="PLW13_14460"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HQY71633.1"
                     /translation="MRPMSRLLLPVVIVTLGGCGWLVGDEGYFRDRGDDYRNAKSIPP
                     MQVPEENKSEAVSQLYVIPVERTDALLDSEFEVPRPQPIQGNPEDKVVRIQKLGEQQW
                     ILLEESPAESWPRVRNFLISNQIGIEREDAGTGQMETGWLSFKSDATRREKYRFRVEQ
                     GVQRASSEIYVQQMGYEKEPGEDASTPEWPAASMDVERETWMIKELANYLASTSGESS
                     VSLLAQGISTVNKVYLVRDASGQPVIDLRLPFDRAWASLGRALERANLSVKDLDRSAG
                     VYFVKYETRKQRAEEGEAGSDQGPGPGAAPEEEEKRSFLSRLNPWGGDDEDENPAIGH
                     SYRVEMRGVDQGVVISVRRDDGEDFDEGEAEFVLGLIKAHLS"
     gene            complement(3783..>4375)
                     /gene="dapA"
                     /locus_tag="PLW13_14465"
     CDS             complement(3783..>4375)
                     /gene="dapA"
                     /locus_tag="PLW13_14465"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQY71634.1"
                     /translation="LLGTPYYNKPSQEGLYQHFRTIAEAVDLPLILYNVPGRTGCDML
                     NDTVLRLCEVPNIVGIKDATGNLDRGRELLSHCPSDFAVYSGDDATAAELMLAGARGN
                     ISVTANVDPARMAALCRAALAGDAARARALDAGLAELNRVLFIESNPIPVKWALLEMG
                     LIGPGIRLPLTPLSPPCQAPVRKALLGLGLLATCEA"
BASE COUNT          712 a         1485 c         1381 g          797 t
ORIGIN      
        1 cgacagccgg cggcagtgcc ttttaccttc gcggccggca cccccttttt ccccgaaagc
       61 ggcagatcag ccgcaacccc ccggtaaact ggccgcctca tcccgtgagg ctgcgccatg
      121 taccgattcg acccgcgtca attcgatctg tcagcctggg cgcggtgggt cgccagtggc
      181 cggccggtcc acttctcgat gctcggcaac tcactctgcg ggcaaccgtt catgccgcag
      241 cacaagcgca cctatcgcga cgactccccg ctatggcagg agcacgacgg cgagatagat
      301 gagcacgtcg ccagactgat gcggatcgac agcgtggttc acggcgctct aagcgtgtct
      361 gagcggcgct tgttgctggc cgcagtagcc ccatcgggta cgggccaccc gctccccctg
      421 gtagagcgtg cgaggctggc gggggcttca ccggaggcgc tgcgggctat caggcgtaag
      481 gctatggcgt tgatcgactg aggtctgccg cgtcgagtcg tcacggtcga aatcgcggaa
      541 aatgtgggaa tcggaatatt ttccgttttc cacaaattcg gcgattttgt cgctcacgac
      601 agttcgatct atccccaccg catccgctat ctcctgctgc gtgtggcagc gcaggtgcat
      661 ggcgtgggcg tgactctggc ggtcgaacta cgctaccgcc agaaacaggc agcgcctcgc
      721 gcgcgtgggc gtgatcctga cgttatgcgg gagcgcggcg gcgtggtgac tcaggccgca
      781 ttagtccgca atcgttcgcc tgcatccgca tatggcagca gggaatagcg tagggaacgg
      841 cgaatggctg aaataaaaaa ggcctatatc tcaaggcctt agtgccattt ctggcggaga
      901 gggtgggatt cgaacccacg gtaggctctc acctacgcct gatttcgagt caggtacatt
      961 cgaccactct gccacctctc caaaactcgc ttgtctttgt cgggctgaag cccgacccac
     1021 agcaggccgc gcattctaca cactcgggcg cgattgagaa acgccccggg aataatgcgc
     1081 ttcagtcgaa gctgagcccc aggcggcggc ccacgatctc gtaggactcc accacgttgc
     1141 ccaggctctg gcggaaacgg tccttgtcga gcttctcgcg cgtctcggcg tcccagatcc
     1201 ggcagccgtc cggcgtgaac tcgtcgccca gcagcacctt gccgtcgcag acgccgaact
     1261 ccagcttgta gtccaccagc agcatgccgt ggtccgaaaa cagcttcttc agcacgccgt
     1321 tgaccttgaa cgtgagctcg cgggcggttt cgacgtcctt caggttcgcc cacttgaagg
     1381 cggcgatgtg gtactcgttg atcatcgggt cgccgagttc gtcgttcttc aggaacagct
     1441 cgaaggtcgg cgggtcgagg tcgatgccct cctttacacc gaggcgccgg cagatgctgc
     1501 cggcggcgat gttgcgcacc acgcattcga ccgggatcat cgccatgcgc cgcaccagcg
     1561 agtcgcgatc atcgatgcgc ttgacgaagt gcgtgggaat gccggccgct tccagcttct
     1621 ccatgatgaa ggcgttgaag atgttgttca cgcggccctt gcgctcgagc tgctcgatcc
     1681 tcttgccgtc gaaggccgag gtgtcgtcgc gaaagtgcat gatgcagtag gcggcgtcat
     1741 cggtggtgta catcgacttg gccttgccct tgacgatcag atcgcgcttt tccatgttcc
     1801 tgtccagttg tggttgcatc gcacgctatt ggatggccag ccagccgaag cccgattcct
     1861 ggtcggccac cagcagcctc tcgtcaccct gccccagcac ctcttccagc gcggcgcgcg
     1921 ccttggcggg ggcgttattc tgttcgctca ggtgcgccgc cacaagatgc tgcaacgcac
     1981 tgcgctccac gccagccagc aacgaggcgg cctgcgcgtt gctcaggtgc ccgaagtcgc
     2041 cgcccacgcg ccgcttcagc gggtatggat acgggccgtt ggccagcatc tcggcgtcgt
     2101 ggttgcactc cagcaccagc gcgtcgcagc gcgcgtactg atcgcgcacc agcttcgaaa
     2161 cgctcccgag gtccgtgagc acgcccaggc tgcgcctggc gtgacccagc acgaactggc
     2221 agggctcgcg cgcatcgtgc ggcaccgtga ccggcagcac ctcgatatcg ccgatgcgct
     2281 cgggacgctc cggaaccagc acgacgggct tcgcgccctc aagccgggca gccttggccg
     2341 tgccggcgct cgcgaatacc gcaatcccgt aatgccgggc cagcgccgcc acgccaccag
     2401 cgtgatcgga gtgttcgtgc gtcaccagga tgccggcgag atcgcgcgga tgcatgccgc
     2461 gctgggccat gcgccgctcc agttcacggc gcccgaaacc gcagtccacc attacgcagg
     2521 tggaggcggt gcggaccagc agggcgttgc cggccgagcc gctgccgagg gaacagaatt
     2581 gcatcagcga aggcccatca tgtcgcgcac agggcgcgct cccacggagt ccaggaccct
     2641 acgacaggtg cgccttgatc aaccccagca cgaactcggc ctcgccctcg tcgaaatcct
     2701 cgccgtcgtc acgtcgcacg ctgatcacca ccccctggtc gacgccgcgc atctccacgc
     2761 ggtagctgtg cccgatcgcg gggttctcat cctcgtcgtc gccgccccac gggttcaggc
     2821 gggagaggaa actccttttc tcctcttcct cgggcgccgc tccggggccg ggaccctgat
     2881 ccgatcccgc ctcgccctcc tcggcccgct gcttgcgcgt ctcgtacttc acgaagtaca
     2941 cgccggcgct gcggtccagg tccttgaccg acaggttggc gcgctcgagc gcgcgcccga
     3001 gcgatgccca ggcgcgatcg aagggcagcc gcagatcgat gaccggctga ccgctggcgt
     3061 cacgcaccag gtagaccttg ttcacggtgc tgatgccctg cgccagcagg ctcacggagg
     3121 attccccgct ggtgctggcc aggtagttcg cgagttcctt gatcatccag gtctcgcgct
     3181 cgacgtccat cgaggcggcc ggccactcgg gcgtggaggc gtcctcgccg ggttccttct
     3241 cgtaacccat ctgctgcaca tagatctcgc tgctcgcgcg ctgcacgccc tgctcgacgc
     3301 ggaagcggta cttctcgcgc cgcgtggcgt cgctcttgaa cgacagccag ccggtttcca
     3361 tctgccccgt gccggcgtcc tcgcgctcga tgccgatctg gttggagatc aggaagttgc
     3421 gcacgcgcgg ccagctctcg gcgggcgact cttccagcaa tatccactgc tgctcaccga
     3481 gcttctggat gcgcaccacc ttgtcttccg ggttgccctg gataggctgc ggacgaggca
     3541 cctcgaactc gctgtcgagc agcgcgtcgg tgcgctccac cggtatcacg tagagctgcg
     3601 acaccgcctc gctcttattc tcctcgggca cctgcatcgg cgggatcgac ttcgcgttgc
     3661 ggtaatcgtc gccgcgatca cggaaatagc cctcgtcgcc caccagccag ccgcagccgc
     3721 ccagcgtgac gatgacaaca ggcagcagca gccgggacat cggtcgcatc aactctctcc
     3781 aactaggctt cgcacgtggc gagcagtcca aggcccagca gcgccttgcg tacgggcgcc
     3841 tggcaggggg gcgacagcgg cgtcagcggc aggcggatgc cggggccgat cagtcccatc
     3901 tccagcaggg cccatttgac cggtatcgga ttcgattcga tgaacagcac gcggttgagc
     3961 tccgcgagtc cggcatcgag cgcgcgcgcg cgcgcggcgt caccggcgag cgcggcgcgg
     4021 cacagcgccg ccatgcgcgc cgggtcgacg ttggcggtca ccgaaatgtt gccgcgcgcg
     4081 ccggcgagca tcagttcggc cgcggtggcg tcatcgccgg agtagaccgc gaaatcgctc
     4141 gggcagtgcg acagcagttc ccgaccgcga tcgaggttgc cggtggcgtc cttgatgccg
     4201 acgatgttgg gcacctcgca caggcgcagc acggtgtcgt tcagcatgtc gcagccggtg
     4261 cgtcccggca cgttatagag gatcagcggc aggtcgaccg cctcggcgat cgtgcggaag
     4321 tgctggtaaa gtccttcctg ggacggcttg ttgtagtagg gcgtgcccag caggc
//
LOCUS       DAORUQ010000228         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_25429_length_4367_cov_5.716141, whole genome shotgun sequence.
ACCESSION   DAORUQ010000228 DAORUQ010000000
VERSION     DAORUQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25429_length_4367_cov_5.716141"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..709)
                     /locus_tag="PLW13_14470"
     CDS             complement(74..709)
                     /locus_tag="PLW13_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY71635.1"
                     /translation="MKRLCLVLSWVVATGAPAIAAAADAARGKTLYAGCVACHGESGA
                     GNEALQAPALAGLDAVYLMRQLHNFKAGIRGADVADTAGAQMRASAALLADEAAMTDV
                     TAYIASFPPPAVAPVAGADLRNGSNYYQASCGACHGGRAEGNTALFSPRLAGQHTAYL
                     KRQYLNFREGLRGAHPDDKYGRQMKVMSAALPAAKDLDDVLGFIAAQGAAE"
     gene            complement(811..1449)
                     /locus_tag="PLW13_14475"
     CDS             complement(811..1449)
                     /locus_tag="PLW13_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQY71636.1"
                     /translation="MRSAEEKQPARPRASAVQRRHNIIEATLRVMARDGLRAVSHRAV
                     ASEASVPLASTTYYFRDLEDLIIESFLHWSEAQRRDIEAFQVRVLALLSQTRVAGTAE
                     ALADAASAYVLDQVQRLRSDRVLEYAFLHEAIRVPRLRAALERQQQGHLEFLRGFHAA
                     LGSPQPELDAQITNSVLLGLEKGALLAQGPVEIRAVMLRHLQQALEPTLSEA"
     gene            complement(1599..2714)
                     /gene="ald"
                     /locus_tag="PLW13_14480"
     CDS             complement(1599..2714)
                     /gene="ald"
                     /locus_tag="PLW13_14480"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQY71637.1"
                     /translation="MLVGVPREIKTHEYRVGLTTGSVSELVHHGHVVIVETMAGAGIG
                     LSDEMYRRAGAEIVGSAAEVFARADMIVKVKEPQPHECRMLREGQVLFTYLHLAPDPE
                     QTRALLDSGCIAIAYETVTGPGNTLPLLSPMSEVAGRMSIQAGAHCLEKEQGGSGMLL
                     GGVPGVEAAKVVVLGGGVVGTNAIRMAMGMEAHVTVIDKSLARLKELDFRFGGMLNTI
                     FATREATQRHVTGADLVVGAVLVPGAAAPKLVTADMVKAMKPGSVLVDVAIDQGGCSE
                     TSHPTTHADPTFIRHGVVHYCVANMPGAVARTSTFALNNATLPFALALADKGYRKAFA
                     DDPHLLDGLNVHRGQLTCQAVAEALGQDWIAARQAIA"
     gene            2876..3874
                     /locus_tag="PLW13_14485"
     CDS             2876..3874
                     /locus_tag="PLW13_14485"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HQY71638.1"
                     /translation="MVTPKTRRLGFVLLVVLALLGTFAWYAFQPRRIDDGQPPWHAAV
                     EQRTIEVAGMSREFLYYAPPGLARGAPMVFVLHGSMGSAAGMREATAFGFERIAAGEG
                     AVVVYPQGIEKHWNDCRGTADYAANTRNIDDPAFFEAMIGWFARELGADRSRVLVTGL
                     SNGGHMAYRLGLERPDLVLAIAPMAAGLPVAATRDCRESGKAIATAIFNGTEDPVNPF
                     AGGLVSILGNSSRGEVLSSQETARFWARLAGHNGEPTIEEMPDRDPDDGTHVRIERWQ
                     APGHAEIAHYMIEGGGHTLPSRGTQPSLATSVLTGRTSHDIDAAHEAWVFFERQWP"
     gene            complement(3963..>4367)
                     /locus_tag="PLW13_14490"
     CDS             complement(3963..>4367)
                     /locus_tag="PLW13_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY71639.1"
                     /translation="GRQDLADDPQLARNAGRAQRETEIDAAIGAWCAARAAAEVLAVM
                     DEKRVPAGPIYNVEDMFADPHFRARGLFERVEIDGKPLEIPALMPRLQGTPGGTEWAG
                     PAVGSHTDAVLGEVLGMDEREIAALRADGVVT"
BASE COUNT          675 a         1536 c         1470 g          686 t
ORIGIN      
        1 cgggcgcagc cagcgccgtg gcgccgggca gcatgccgag cagcgcgaca cacgggagcc
       61 agcgcatcgc gcgctactcc gccgcgccct gcgcggcgat gaagcccagc acgtcgtcga
      121 gatccttcgc cgccgggagc gccgcggaca tcaccttcat ctgccggccg tacttgtcat
      181 ccgggtgcgc gccgcgcagc ccctcgcgga aattgaggta ctggcgcttg aggtatgccg
      241 tgtgctggcc ggcgaggcgc ggcgagaaca aggcggtgtt gccctcggcg cgcccgccgt
      301 ggcaggcacc gcacgaggcc tggtagtaat tcgaaccgtt gcgcagatcg gcgcccgcga
      361 ccggcgccac cgcgggcggc gggaacgagg caatgtacgc cgtcacgtcg gtcatcgccg
      421 cttcatccgc gagcagcgcg gcggaggccc gcatctgcgc accggcggta tctgcgacat
      481 cggcgccgcg aatgcccgcc ttgaagttgt gcagttgccg catgagatac accgcatcca
      541 gtcccgccag cgcgggcgcc tgcagcgcct cgttgccggc gccggactcg ccgtggcagg
      601 ccacgcagcc cgcgtagagc gtcttgccgc gcgcggcatc cgccgccgcc gcgattgccg
      661 gagcgccggt ggcaaccacc catgaaagga ccaggcacag gcgtttcatc tggttattct
      721 ccgaaagccg cccgcgggcg gcacgccaaa gtggtacagt tgtaccattt cagcaattgc
      781 cgccgcaacc ggaattattg cgcggcgcac tcaggcctcg gagagtgtcg gctccagtgc
      841 ctgctgcagg tggcgcaaca tcacggcgcg gatctctacc ggcccctgcg ccagcagcgc
      901 gcccttctcc agcccgagca gcacgctgtt ggtgatctgg gcgtccagct ccggctgcgg
      961 cgagccgagc gcggcatgga acccgcgcag gaattccagg tggccctgtt gctggcgttc
     1021 cagcgcggcg cgcaggcgcg gcacgcgtat cgcctcgtgc aggaatgcgt actcgagcac
     1081 gcggtcactg cgcaggcgct gcacctggtc cagcacgtag gcgctcgcgg cgtcggccag
     1141 cgcctcggcc gtgccggcca cgcgcgtctg gctcagcagc gccagcacgc gcacctggaa
     1201 agcctcgatg tcgcggcgct gtgcttccga ccagtgcagg aaggactcga tgatcaggtc
     1261 ctcgagatcg cggaagtaat aggtggtgct cgccagcggc accgaggcct cggacgccac
     1321 cgcgcggtgg ctcaccgcgc gcagcccgtc gcgtgccatc acccgcaggg tggcctcgat
     1381 gatgttgtgg cgccgctgta ccgcgctcgc gcgcggccgc gccggctgtt tttcttccgc
     1441 ggatcgcatg cgcggattct acaggctgag gaactcagct cgtagaccga ttgcattcgg
     1501 actttgtgcg ttgctccgat tgcattcgga cattgtgtag gtccggattt atccggacga
     1561 ttgtccggct gaagccggac cgcccgaccc atgagggatc aggcgatggc ctgccgcgcc
     1621 gcaatccagt cctgccccag cgcctcggcg acggcctggc aggtgagctg gccgcgatgc
     1681 acgttcagtc cgtccagcag gtgcgggtcg tccgcgaacg ccttcctgta gcccttgtcg
     1741 gccagcgcga gcgcgaaggg cagcgtggcg ttgttcagcg cgaaggtcga ggtgcgcgcc
     1801 accgcgcccg gcatgttcgc cacgcagtaa tgcaccacgc cgtgcctgat gaaggtgggg
     1861 tcggcgtgcg tggtcgggtg gctggtctcg gagcagccgc cctggtcgat cgccacatcg
     1921 accagcaccg atccgggctt catcgccttg accatgtccg cggtcaccag tttcggcgcc
     1981 gcggcgccgg gcaccagcac cgcgccgacc acgagatcgg cgccggtcac gtggcgctgc
     2041 gtggcttcgc gcgtggcgaa gatcgtgttc aacatgccgc cgaagcggaa gtcgagttcc
     2101 ttcagtcgcg cgagcgactt gtcgatcacg gtcacgtgcg cctccatgcc catggccatg
     2161 cggatcgcgt tggtgccgac cacgccgccg cccagcacca ccaccttcgc ggcttccaca
     2221 cccggcacgc cgccgagcag catccccgag ccgccctgct ccttctccag gcaatgcgca
     2281 ccggcctgta tcgacatgcg cccggcaacc tcgctcatcg gcgacagcag cggcagcgta
     2341 ttaccgggac cggtgacggt ctcgtaggcg atcgcgatgc agcccgagtc gagcagcgcc
     2401 ctcgtctgct ccggatccgg cgcgaggtgc agataggtga acagcacctg gccttcgcgc
     2461 agcatgcggc actcgtgcgg ctgcggctcc ttgaccttca cgatcatgtc ggcgcgcgcg
     2521 aagacctcgg cggcgctgcc gacgatctcg gctccggcgc ggcggtacat ttcgtcactg
     2581 aggccgatgc cggcgccggc catcgtctcg acgatcacca cgtggccgtg atgcaccagc
     2641 tcgctgacac tgccggtggt caggccgaca cggtattcgt gcgttttgat ttccctgggc
     2701 acgccgacga gcatgggtcg attctccggg gatggaagga tgccggttat ccggactctg
     2761 ccagtataag caggcattgt gcgcgatgtt tcgccgaagg ccacccccga cggggtatgc
     2821 tgcacagcgc aaacgacagc agccaatggc tgcagcgcaa aggaaggaca agtgcatggt
     2881 tacaccgaag acccgccgcc tcggcttcgt gctactcgtc gtcctggcgc tgctcggcac
     2941 tttcgcgtgg tacgccttcc agccgcggcg catcgacgac gggcagccac cgtggcatgc
     3001 agcggtcgag cagcgcacga tcgaggtcgc gggcatgagc cgcgaattcc tctactacgc
     3061 gccaccgggc ctcgcacgcg gggcgccgat ggtcttcgtg ctgcacggat ccatgggcag
     3121 cgccgccgga atgcgcgagg ccactgcctt cggcttcgag cgcatcgccg ccggtgaggg
     3181 cgcggtcgtg gtctacccgc agggaatcga gaagcactgg aacgactgcc ggggtactgc
     3241 ggactacgcc gcgaacacac gcaacatcga cgacccggcg ttcttcgagg ccatgatcgg
     3301 ctggtttgcg cgggaactcg gcgccgatcg ctcgcgcgtg ctggttaccg gcctgtcgaa
     3361 cggcggacac atggcctacc ggctggggct ggagcgcccg gatctcgtgc tggcgatagc
     3421 gccgatggcc gcggggctgc cggtagccgc cacgcgagac tgccgcgagt cgggcaaggc
     3481 gattgccacc gcgatcttca acggcaccga ggatccggtg aaccctttcg cgggcggcct
     3541 cgtgtccata ctcggtaaca gcagccgcgg cgaggtcctc tcatcgcagg agaccgcgcg
     3601 cttttgggcg cgacttgcag ggcacaatgg cgagccaacg atcgaggaga tgcccgatcg
     3661 cgaccccgac gacggcacgc acgtgcgaat cgagcgctgg caggcgccgg gtcacgcgga
     3721 gatcgcgcac tacatgatcg agggcggcgg acacacactg ccatcccgag gcacgcaacc
     3781 gtcgctcgcc accagtgtcc tgaccggccg caccagccac gacatcgatg ccgcgcacga
     3841 ggcgtgggtg ttcttcgagc ggcagtggcc gtagctccgg acgtgggtcc ggattcatcc
     3901 ggacagatgt ccgcaccacc tggatccgga ttcatccgga caatgtccgg ttgaaaccgg
     3961 acctacgtga ccaccccatc ggcgcgcagc gcggcgatct cccgctcgtc catccccagc
     4021 acctcgccga gcacggcatc ggtatggctg cccaccgccg ggccggccca ctcggtgccg
     4081 ccgggcgtgc cctgcaggcg cggcatcagc gccgggatct ccagcggctt gccgtcgatc
     4141 tccacgcgct cgaacaagcc gcgcgcgcgg aaatgcgggt cggcgaacat gtcctcgacg
     4201 ttgtagatcg gcccggcggg cacgcgcttt tcgtccatca ccgcgagcac ctcggcggca
     4261 gcacgcgcgg cgcaccaggc gccgatcgcg gcatcgatct ccgtctcgcg ctgcgcccgc
     4321 ccggcattgc gcgcgagctg cggatcgtcg gccaggtcct ggcggcc
//
LOCUS       DAORUQ010000229         4364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_25456_length_4364_cov_6.783244, whole genome shotgun sequence.
ACCESSION   DAORUQ010000229 DAORUQ010000000
VERSION     DAORUQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25456_length_4364_cov_6.783244"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLW13_14495"
     CDS             complement(<1..428)
                     /locus_tag="PLW13_14495"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQY71640.1"
                     /translation="MLTLNDSSIIALSHIIQVSVAPVFLLTGIGSILGVLANRLGRIV
                     DRARFLESRPELSRHLTEASAEVELLRLAERKRWVNRAITLCTLCALLVCTVIATLFV
                     GAFVTLEIGTIVAALFILSMLALIAGLICFLREIYVAIHK"
     gene            658..1212
                     /locus_tag="PLW13_14500"
     CDS             658..1212
                     /locus_tag="PLW13_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQY71641.1"
                     /translation="MKRYFYIDDDLDRLEKVERDLLAAGVSAPQIHVLSQDDAGVTRH
                     HLHQVHSLLRQDTIHSGEIGALIGLGISALALALGWLGGLPDHIGWVPFGFLCVVLLG
                     FFTWEGGLIGIQAPNSQFRRFANALAQGRHLLIVDAEPAQEKPLRTIVARHPGLEAAG
                     TGTATPRWLVHWQRRWQDFLQWAP"
     gene            1516..1716
                     /locus_tag="PLW13_14505"
     CDS             1516..1716
                     /locus_tag="PLW13_14505"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HQY71642.1"
                     /translation="MEDRFENLESKMMSAEDQIDELNRTVWRQQQALDVLRDQLRQLA
                     QQLKSVQEALPAPPGQEIPPHY"
     gene            1758..2546
                     /locus_tag="PLW13_14510"
     CDS             1758..2546
                     /locus_tag="PLW13_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71643.1"
                     /translation="MNDSLLLGNADCRVRVDAARGGRIASLCVYGEELLVAAGDDPLA
                     WGSYPLAPWAGRLREGQFRFAGREYRMPCDSGAHAIHGTVFRRGWQIVAGDTLRIELQ
                     PDWPFDGFALQRLRLHDHALEVRLELHARTQPYPASAGFHPWFRRRLRRGAPLALDLG
                     ATRRIVLDGEGLPTGALATPASATGDDCFIDLQRDPCLHWPGVLRLRLSSNHAHWVVY
                     EHPAHAVCVEPWTAPPDALNGTPALVRPGAPLVLEMRLRWERDA"
     gene            complement(2600..3106)
                     /locus_tag="PLW13_14515"
     CDS             complement(2600..3106)
                     /locus_tag="PLW13_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71644.1"
                     /translation="MSTKSVPAALAVVAAVALTLAACGKKEEPPQAQVAPPPVSMPAP
                     PAAEPAPPVAAAPAEVAVATLTLGNAVGADARVSAPTETFAPADTIYVSVDTTGAGTA
                     AMAVKWTYHQDGQVAVVKEELISIAPTGPATTEFHVSKPDGWPAGNYAVEIVLNGKPA
                     GSRTFVVR"
     gene            complement(3147..3296)
                     /locus_tag="PLW13_14520"
                     /pseudo
     CDS             complement(3147..3296)
                     /locus_tag="PLW13_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120410.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(3467..>4364)
                     /locus_tag="PLW13_14525"
     CDS             complement(3467..>4364)
                     /locus_tag="PLW13_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71645.1"
                     /translation="LIFAFGALPPGQSAPALIARLQGCQDPVTGMPADPLAPPRAGYV
                     AAQMNDANTAYMVLSLGYALMCLGARFRQPFGVPLAMEPAQLQELLARQPWASNAWGA
                     GAWVDALGTALWMNRYCFGQPGPEGALFAWLQGACNPQTGLWGEPRAQDGWLEPVNGF
                     YRLTRGTYAQFDRPVPWPEAALDTILAHIRANDGFATRNVNACNLLDVVHPLWLLSQS
                     SAHRRDEVLAFIEAQLPLIAARWIDGAGFAFAPGGAAGLQGTEMWLSILRIGADVLGM
                     GDELPWTPRGVHRLRPPGAAHD"
BASE COUNT          667 a         1509 c         1515 g          673 t
ORIGIN      
        1 tccttgtgga tggcgacgta gatctcgcgc aggaaacaga tcaggccggc gatcagcgcc
       61 agcatcgaga ggatgaacag cgcggcgacg atggtgccga tctccagcgt gacgaaggcg
      121 cccacgaaga gcgtcgcgat cacggtgcag accagcagcg cgcacagcgt gcacagcgtg
      181 atcgcgcggt tgacccagcg cttgcgctcg gcaagacgca gcagctcgac ctcggcgctg
      241 gcctcggtca ggtggcgcga caactcgggg cgcgactcga ggaaacgcgc gcgatcgacg
      301 atgcgcccca ggcggttggc gagcacgccg agaatcgagc cgatgcctgt cagcaggaac
      361 accggcgcca cggacacctg gatgatgtgc gagagcgcga tgatgctgga atcgttgaga
      421 gtcaacatgg cgttagcggc ccgggcgtga atgccgcgca ttgaaacgca agcacgccgc
      481 cgcgacaagc cgggttgcac atgccatgcg gacctgaagg ggcaccctga aattccactt
      541 tcgactaatc ctgccgtgga tacggggaat acaatgcgag tcggttcgca agggcacggc
      601 ttgccgtggt gcccgctgcg cgtgatccac caccagggca tcgaccggag gtgtgtaatg
      661 aaacgttact tctacatcga cgacgacctg gaccgcctgg agaaggtcga gcgcgatctg
      721 ctcgccgcgg gcgtctcggc cccgcagatc cacgtgctga gccaggacga tgcgggtgtc
      781 acccggcatc acctgcacca ggtgcattcg ctgttgcgcc aggacacgat ccattccggc
      841 gagatcggcg ccctcatcgg gctgggcatc tcggcgctgg cgctcgcgct cggctggctc
      901 ggcggactgc ccgatcacat cgggtgggtt cccttcggct ttctctgcgt ggtgctgctg
      961 ggcttcttca cctgggaagg cggactgatc ggcatccagg cgccgaactc gcagttccgc
     1021 cgtttcgcca atgcgctggc gcagggccgt cacctgctga tcgtcgacgc cgaacccgcg
     1081 caggaaaagc cgctgcgaac gatcgtggcg cgccatcccg ggctggaggc ggcgggaacc
     1141 ggcacggcaa caccgcgctg gctcgtgcac tggcaacgcc gctggcagga tttcctgcaa
     1201 tgggcgccct gatcgcggga tgctgtaacg cgtgctcgcg ccgctttcag ctcgccctcg
     1261 tgaccgaggc ggtcggtgtg cacctcgccc ggcgtcaaag acgatgtcct cctcgacgcg
     1321 ccagcgtcat cgcggacagg caggcgcagg ccgcttcgag cgctgcgcgc ggcgctgtcg
     1381 ccgcggcttg cgcggcaggc ggcgcgtcct tgtgcgcgca ttggatcgac ccgccgcatt
     1441 gtcgcagatc ggacgcgccc tgccctcctg cggccaacgc ggctagaatc gccgctcccg
     1501 ccacaggacc cgcccatgga agaccgtttc gagaatctcg agtccaagat gatgtccgcc
     1561 gaggaccaga tcgacgagct caaccgcacg gtgtggcgcc agcagcaggc gctggacgtg
     1621 ctgcgcgacc agttgcgtca actcgcgcag cagctgaaga gcgtgcagga ggcgcttccg
     1681 gcgcctcccg ggcaggagat tcccccgcac tactgagtgc gcgggcgcgt ggcgcgacgg
     1741 ccaacggaac acctgcaatg aacgacagcc tgctgctggg caacgccgac tgccgcgtgc
     1801 gcgtcgatgc ggcgcgcggc ggccgcatcg cctcgctgtg cgtctatggc gaggagttgc
     1861 tggtcgcggc cggcgatgac ccgctcgcgt ggggcagcta tccgctggcg ccgtgggcgg
     1921 gacggctgcg cgagggccaa ttccgcttcg cgggccgcga gtaccggatg ccctgcgatt
     1981 ccggcgcgca cgcgatccac ggcacggtgt tcaggcgcgg ctggcagatc gttgccgggg
     2041 acacattgcg catcgagctg cagcccgact ggcctttcga cggcttcgcg ctgcagcgct
     2101 tgcggctgca cgaccacgca ctcgaggtgc ggctggaact gcacgcgcgc acccagccct
     2161 accccgccag cgcgggtttc catccctggt tccggcgtcg attgcgccgc ggcgcgccgc
     2221 tcgcgctcga cctcggcgcg acgcggcgca tcgtgctcga cggcgagggc ctgcccaccg
     2281 gggcgctcgc aacccccgcc agcgccacgg gtgacgactg cttcatcgac ctccagcggg
     2341 atccctgcct gcactggccc ggcgtgctgc gcttgcggct ttcgtcgaac catgcccact
     2401 gggtggtcta cgagcacccg gcgcacgccg tctgcgtgga gccgtggacc gcgccgcccg
     2461 atgcgctgaa cggcacgccg gcgctggttc gccccggcgc gccgctggta ctggagatgc
     2521 gcctgcgctg ggagcgggat gcgtaacggc tacgtcacgc ggacaggcga tcagcgcctg
     2581 ccggcgtgag cgagcgtctt cagcggacga cgaaggtgcg gctgccggcg ggcttgccgt
     2641 tcaggacgat ctcgaccgcg tagttccccg ccggccagcc gtcgggcttg ctcacgtgga
     2701 actcggtcgt ggccggaccg gtgggcgcga tggagatcaa ctcctccttc acgacggcaa
     2761 cctgcccgtc ctggtggtag gtccacttga ccgccatcgc tgccgtgccg gcaccggtgg
     2821 tgtcgacgga cacatagatc gtgtccgcgg gcgcgaaggt ctcggtcggt gccgataccc
     2881 tggcgtcggc gccgacggca ttgcccagtg tcagcgtggc gacggcgact tcagccggtg
     2941 ccgcggcaac cggcggcgcc ggttcggcgg ccggcggcgc cggcatgctc acgggtggcg
     3001 gcgcgacctg cgcctgcggt ggctcttcct tcttgccgca ggcggcaagc gtgagcgcga
     3061 cggcggcaac gaccgcgagg gccgcgggta cggacttggt gctcatcatc gggttctccc
     3121 tgtctgcgtg tgcgaatgcc tgctggtcac tgcggcggga tcttcagctt ctggccgggg
     3181 aagatcttgt cggggtcgtc gaggatgtcc ctgttcgcct cgaagatgcg cttccagtcg
     3241 ccggcgcgac cgtactcatg cttggcgatc ttcgacaggg tgtccccttt caccaccacg
     3301 tagaccttcg gcgcggtcga actggaaccg ctcttgacgt tggagaagtc gggcatgtcc
     3361 ttgctcatgg attcctcctg cggaacaaca gtcggagcga ccggtatggc gcgccctgcg
     3421 ccgacggtat accacgtcct tcgcctgcac cggaagggcg cgccgatcag tcgtgcgcgg
     3481 cgccgggcgg gcgcaggcgg tgcacgcccc gcggcgtcca gggcagttca tcgcccatcc
     3541 ccagcacgtc cgcgccgatc ctgaggatcg acagccacat ctccgtgccc tgcaaccccg
     3601 cggcgccccc gggcgcaaag gcgaagccgg cgccgtcgat ccagcgcgcg gcgatgagcg
     3661 gcagttgcgc ttcgatgaat gccagcacct cgtcgcggcg gtgcgcgctc gactgcgaga
     3721 gcagccacag cgggtgcacc acgtcgagca ggttgcaggc gttgacgttg cgcgtcgcga
     3781 aaccgtcgtt ggcgcggatg tgcgcaagga tcgtgtcaag cgccgcctcg ggccacggca
     3841 cgggccggtc gaactgcgcg taggtgccac gggtcagccg gtagaaacca ttcaccggct
     3901 ccagccagcc gtcctgcgcc cgaggctcgc cccacaggcc cgtctgcgga ttgcaggcgc
     3961 cttggagcca cgcgaacagc gcgccctcgg gacccggctg accgaagcaa taccggttca
     4021 tccacagcgc cgtgcccagg gcatcgaccc aggcgcccgc gccccaggca ttgctggccc
     4081 acggttgcct ggcaagcagt tcctgcaact gcgccggctc catcgccagc ggcaccccga
     4141 agggctgccg gaaacgcgcg cccaggcaca tcagcgcgta gcccagcgac agcaccatgt
     4201 aggcggtgtt ggcgtcgttc atctgcgccg cgacataccc ggcacgcggt ggcgccagcg
     4261 gatccgcggg catcccggtt acgggatcct ggcagccctg caggcgcgcg atcagcgcgg
     4321 gcgcggactg ccccggcggc agggcgccga acgcgaaaat cagc
//
LOCUS       DAORUQ010000230         4330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_25793_length_4330_cov_6.248889, whole genome shotgun sequence.
ACCESSION   DAORUQ010000230 DAORUQ010000000
VERSION     DAORUQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25793_length_4330_cov_6.248889"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2346
                     /locus_tag="PLW13_14530"
     CDS             <1..2346
                     /locus_tag="PLW13_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY71646.1"
                     /translation="ALDARAEDFQSRPGILLAVPGREAVANLAPSPPANGTLVMEHLN
                     DVRFPAAWNTQALQGDCADPVTVVVVDYFRRPVPDSSVYQDFGLEVPGVQYAGSTRLP
                     QAGFQYHGYDVLGTLAAAIDDAVPTGANPFPGCLTVRAINVAGQTLLADNFAIQQAIP
                     ASGKAVVNTSLGFDGCGDPCTPANLNIARAVDRALTGAHVRSFVRELEERVLLTTAAG
                     NENEEAVGAIYPGARLAPWTYATTVASRADADFSFATDAQLWEPTVECPPGPCFPSLT
                     MGPQDQTRLDDLLSQLALTGADAPGNILIVGAVDPNQNLRAGYSEPGADLAAVGTVPL
                     MEPDVFELGTSFAAPQVAGLASYLWRISPELRNGPVADTIGAILANTRGAESVIDAYA
                     TVLSLDPVGAPDPATWHIRRTLLDVDNDGGFDEGDLQLYVERFYEAPAYEDEVNPAAR
                     DYSEYDLNGDGYTGSNRLESFDLDRSGSSRYGVPTLTTLQLEVGTERLEIDENNASDL
                     DILCWYAWSPLYAGDPDAREEILANRCFTISVTVDPANASVQPGGTVQFAADVSGTDN
                     PAVDWALPDGGGTIDPDGLFTAGNEAGTFTVRAISQVDPNAFGDATVQVGAGQPSGVV
                     TLMTQGWSQPQENPITSRVDHTYSGTITARFEGTAATITAATGQVTKFTESHRNGCIS
                     TTTYSGTVTGWAIASYQVFGYSVLELYATGKWESSGVTFGGVDPQGNIICTPVGPPPA
                     TPSATQGLWFSYVRAADGTVTGLDFNVTMGDPPTVQTGVVD"
     gene            complement(2390..3379)
                     /locus_tag="PLW13_14535"
     CDS             complement(2390..3379)
                     /locus_tag="PLW13_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY71647.1"
                     /translation="MAYVSVKYECRAPAATTAVSLAQMYDTTLEQIAWIDSLGLPVTI
                     NFCEHHGAEDGYLPSPLLMAAAAARLTRHVRLQVNVLLPYLDPLRVAEDVAVLDHLSH
                     GRAEVLLLGGYVASELAMFGVEARERGTLMEEGVRALKEAWTGEPFTYRGRPARIFPR
                     PLQQPHPPIFMGGSAAPAARRAAREGDAFIGGMPHLNEIYASECAALGKPPRFQGELP
                     MGFLYVSEDPERDWARIAPYCLYETNCYARWQQSSSVDMLFGSAVSTDELRKLGTYQV
                     LRAEDVLARAARMAPNDCIMLHPLVGGLDADLSWETLRNFFDKVAPRLNINGI"
     gene            3563..>4330
                     /locus_tag="PLW13_14540"
     CDS             3563..>4330
                     /locus_tag="PLW13_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY71648.1"
                     /translation="MAEQKLTFCRICENQCGLAVTVEGDRITQVAPDTGHVASRGFAC
                     NKGLTLENVRASPDRLTTPLKKVDGRFEPIGWSQALDEIGTRLRAIRARHGDDAVGLY
                     FGNPISFSLLMPMLITGFAQGLNTAKVFTTGSLDCNNKFLVGKHMYGSPMALTFPDVD
                     ATRFLVIIGGNPAISKMSFINLPDPVRRLEGIVQRGGRVVHLNPRRTETAKAVGEQVF
                     IRPDTDVFFLLAFLQEVIARDGIRREAVETHMNGFAQV"
BASE COUNT          707 a         1537 c         1429 g          657 t
ORIGIN      
        1 gcgctggacg cgcgcgccga agacttccag tcccgccccg gcatcctgct cgcggtgccc
       61 ggccgcgagg cggtcgcgaa cctggcgccc tcgccgccag ctaacggcac cctcgtcatg
      121 gagcacctga acgacgtacg tttccccgcg gcatggaaca cgcaggcact gcagggcgat
      181 tgcgcggacc cggtgaccgt cgtcgtggtg gactatttcc gccggcccgt gccggactcc
      241 agcgtgtacc aggacttcgg cctggaggtg ccgggcgtgc agtacgccgg cagcacgcgg
      301 ctgccacaag cgggcttcca gtatcacggc tatgacgtcc tcggcacgct ggcggccgcg
      361 atcgacgacg ccgtgcctac cggcgcaaac cctttccccg gctgcctgac tgtcagggcc
      421 atcaacgtcg ccgggcagac gttgctcgcg gacaacttcg ccatccagca ggcgattccg
      481 gccagcggca aggcggtggt gaatacctcg ctcggcttcg acggctgcgg ggatccgtgc
      541 acgcccgcga acctgaacat cgcccgtgcc gtggaccgtg cgctcaccgg ggcgcatgtg
      601 cgctcgttcg tgcgcgaact ggaggagcgc gtgctgctca ccaccgccgc gggcaacgaa
      661 aatgaagagg ccgtgggcgc gatctacccc ggggcgcgcc tcgcgccgtg gacctacgcc
      721 accaccgtcg cgtctcgggc ggacgcggac ttcagtttcg ccaccgacgc gcagctgtgg
      781 gagcccaccg tcgagtgccc gccgggtccc tgcttcccct cgttgacgat ggggccgcag
      841 gaccagacgc gactcgacga tctcctcagc cagctcgccc tcaccggagc cgacgcaccg
      901 ggcaacatcc tcatcgtggg cgccgtggat ccgaaccaga acctgcgcgc cggctactcc
      961 gagcccgggg cagacctggc cgcggtgggc accgtgccct tgatggagcc agacgtgttc
     1021 gagctgggca ccagtttcgc ggcaccgcag gtggcggggc tcgcgagcta cctgtggcgg
     1081 atttcgccgg aactgcgtaa cggcccggtg gcggacacca tcggcgccat tctcgccaac
     1141 acccggggag cggaatccgt catcgacgcc tacgcgacgg tgttgtcgct ggatcccgtg
     1201 ggcgctcccg accccgccac ctggcacatc cgccggaccc tgctggacgt ggacaacgac
     1261 ggaggcttcg acgaagggga cctgcaactc tacgtggagc gtttctacga ggccccggcc
     1321 tacgaggacg aggtgaatcc cgccgcgcgc gactacagcg aatacgacct gaacggcgac
     1381 ggctacaccg gcagcaaccg tctcgagtcc ttcgacctcg accgctccgg atccagccgc
     1441 tacggggtgc caaccctcac gacgttgcag ctggaggttg gcaccgagcg cctggagatc
     1501 gacgagaaca acgcctcgga tctcgacatc ctgtgctggt acgcgtggtc gccgctctac
     1561 gcaggggacc cggacgcccg tgaggagatc ctcgcgaacc gctgtttcac catttctgtc
     1621 accgtggatc ccgccaatgc cagcgtccag ccgggcggca cggtgcagtt cgccgccgac
     1681 gtcagcggca ccgacaaccc cgcggtggac tgggcgctgc cggatggtgg cggcaccatc
     1741 gacccggacg gactgttcac cgcaggcaac gaagcgggca cgttcaccgt gcgggccatt
     1801 agccaggtcg acccgaacgc cttcggcgat gcgacggtcc aggtcggcgc cggacagccg
     1861 agcggcgtcg tgacgttgat gacgcagggc tggagccagc cgcaggagaa cccgatcacc
     1921 tcccgggtcg accacacgta ctccggcacg attaccgccc gcttcgaggg aacggccgcc
     1981 acgatcaccg cggccaccgg gcaggtcacc aagttcaccg agtcgcatcg caacggctgc
     2041 atcagcacca ctacatacag cggcaccgtc acggggtggg cgatcgcctc ctaccaggtc
     2101 ttcggttact ccgtgctgga actgtacgcg accggaaaat gggagagttc gggcgtgacg
     2161 ttcggcggcg tggatccgca gggcaacatc atctgcacac ccgtgggtcc gccgccggcg
     2221 acgccgagcg ccacccaggg attgtggttc agttacgtcc gggcggcaga cggcacagtc
     2281 accgggctgg acttcaacgt gacgatgggc gatccgccga cggtgcagac gggcgtggtc
     2341 gactgacgcc ggggccgaac gccgatcgcc ccgatgttcg cgacacggct caaataccgt
     2401 tgatgttcag ccgtggcgcc accttgtcga agaagttgcg cagcgtttcc cagctcaggt
     2461 ccgcatcgag cccgcccacc agcgggtgca gcatgatgca gtcgttcggc gccatccgcg
     2521 ccgcgcgcgc cagcacgtcc tccgcgcgca gcacctggta ggtgccgagc ttcctcaact
     2581 cgtcggtgct caccgccgag ccgaacagca tgtccacgct gctgctctgc tgccagcgcg
     2641 cgtagcagtt ggtctcgtag aggcagtagg gcgcgatgcg cgcccagtcg cgctcgggat
     2701 cttcggacac gtacaggaag cccatcggca gctcgccctg gaagcgcggc ggcttgccca
     2761 gcgccgcgca ctcgctcgcg tagatctcgt tcaggtgcgg catgccgccg atgaaggcat
     2821 cgccctcgcg cgccgcgcgg cgcgcggccg gtgcggcgct gccgcccatg aagatcggcg
     2881 ggtgcggttg ctgcagcgga cgcgggaaga tgcgcgcggg gcggccgcgg taggtgaagg
     2941 gctcgcccgt ccatgcttcc ttaagcgcac gtacgccctc ctccatcagc gtgccgcgct
     3001 cacgtgcttc cacgccgaac atcgcgagtt ccgaggccac gtagccgccc agcagcagta
     3061 cctcggcgcg gccgtggctc aggtgatcga gcaccgccac gtcctcggcc acgcgcagcg
     3121 ggtccaggta gggcagcagc acgttcacct gcaggcgcac gtgacgcgtg aggcgcgcgg
     3181 ccgccgcggc catcagcagc gggctcggca ggtagccgtc ctcggcgccg tggtgctcgc
     3241 agaagttgat cgtgacgggc aggccgagcg aatcgatcca ggcgatctgc tccagcgtgg
     3301 tgtcgtacat ctgcgccagc gacaccgcgg tggtggcggc cggtgcccgg cattcgtatt
     3361 tcacactgac ataggccatc gggttcctcg taccggtcgg ttgttttgcg agcgcgcatg
     3421 atcgcaggcc aggcggcgca gggcgagccg ggcgcgcggg cgccatgctg tccgaggtgt
     3481 aatcaacgcg cactgcccgc gccggcataa tcggccgcgg cgcatgccgc ccataacaac
     3541 aaccgccaca cgggacgccg tcatggccga acagaagctc acgttctgcc gcatctgcga
     3601 gaaccagtgc ggcctcgccg tcaccgttga gggcgatcgc atcacgcagg tcgcgccgga
     3661 caccggacac gtggcctcgc gcggcttcgc ctgcaacaag ggcctcacgc tcgagaacgt
     3721 gcgcgcctcg cccgatcgcc tcaccacgcc gctgaagaag gtcgacggtc gtttcgagcc
     3781 gatcggctgg tcgcaggccc tcgatgaaat cggcacccgc ctgcgcgcca ttcgcgcacg
     3841 tcacggcgac gatgcggtcg ggctctattt cggcaacccg atcagcttca gcctgctgat
     3901 gccgatgctg atcaccggct tcgcgcaggg cctgaacacc gccaaggtct tcaccaccgg
     3961 cagcctcgac tgcaacaaca agttcctggt cggcaagcac atgtacggct cgccgatggc
     4021 actgaccttt cccgatgtcg atgccacgcg cttcctggtg atcatcggcg gcaacccggc
     4081 catctccaag atgagcttca tcaacctgcc ggacccggtg cgccgcctcg agggcatcgt
     4141 gcagcgcggg gggcgcgtgg ttcacctcaa cccgcgccgc accgagacgg caaaggccgt
     4201 tggcgagcag gtgttcatcc ggcccgacac cgacgtgttc ttcctgctgg cgttcctcca
     4261 ggaagtcatc gcgcgcgacg gcatccggcg cgaggcggtc gagacgcaca tgaacggctt
     4321 cgcgcaggtc
//
LOCUS       DAORUQ010000231         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26058_length_4300_cov_7.807774, whole genome shotgun sequence.
ACCESSION   DAORUQ010000231 DAORUQ010000000
VERSION     DAORUQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26058_length_4300_cov_7.807774"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PLW13_14545"
     CDS             <1..1052
                     /locus_tag="PLW13_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007997428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQY71649.1"
                     /translation="FCDALKQAGEQIQRADAPSDPFTRAEGYRYLSRLARAGLEWYVE
                     FNDPAFPVLYKPSHETIKIGADNPDNIYQKAVVDGRHDYVIRGMRGSVYYISMATSKG
                     SYAENFKQIETGFLDSNSLQIAADGSFEIILSATPKPGNWLPMHADSQSLLIRQTFMD
                     RASEIPAEITIERADAGATPEPLSAETLDRKLRTAASFIDNTARLFADWSASYRGRPN
                     ELPPADQQLCHATGGDPNIFYYHGYFELAEDEALVIEVERIPECRAWNLQTDNYWMES
                     LDYRYHNIHLNKHTAKSRADGGVTMVLAHRDPGLDNWLDTAGHRLGTLCFRWIGAKEV
                     VHPTTRVVKFADLRA"
     gene            1058..2239
                     /locus_tag="PLW13_14550"
     CDS             1058..2239
                     /locus_tag="PLW13_14550"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71650.1"
                     /translation="MTADRLNDEQLLEEARRETGFEDFGDPSFREPMRRLIEALNGEA
                     RLNDTGRAYFAGRIRSVLITRLRAEDWFRRHPEILEEEIGAPLAIVGLPRTGTTMMHR
                     TISADHRMYSPLWYEVRYPVPMPGTDFASGNDPRIALAEAEVRAMLEASPELASIHPM
                     DARGPDEDIMLLEQSFFSTVPESYAHMPDFGVWLERQDQRPGYRYLKRLLQFLQWQKK
                     QRGLEAERWVLKAPHHIHFPAALFETFPDVKVLQTHRDPLQLMPSYGSMMYALTYPFS
                     DAADKAAIAKHWCEKWATGMHATMRFRDAGHDDRFLDMWYEDTVSSPLDEIQRLYNFV
                     GVDLTAEARAEMARWRDMNRREARPTHQYTLEEYGFTEEGLKNTFREYRERYILSRRL
                     G"
     gene            2398..3015
                     /gene="cysC"
                     /locus_tag="PLW13_14555"
     CDS             2398..3015
                     /gene="cysC"
                     /locus_tag="PLW13_14555"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HQY71651.1"
                     /translation="MTEQKATNVHWHEGEIQREHRHKLLGQQGATLWFTGLSGSGKST
                     VAVALEEALYLRGKLAYRLDGDNIRLGINKNLGFSADDRKENIRRIGEVAKLFVDCGV
                     IVLTSFISPYRSDRDIVRQLHAASDMPFIEVHVDCALAEAEKRDPKGLYKKARAGEIK
                     NFTGIDDPYEAPEAPEIHLHTDRMTLQQEVDMILHELEKRGILSA"
     gene            3034..4224
                     /gene="sat"
                     /locus_tag="PLW13_14560"
     CDS             3034..4224
                     /gene="sat"
                     /locus_tag="PLW13_14560"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HQY71652.1"
                     /translation="MIKPHGSDALDPRFVYDTERHHALMKEAESLPSLLLNSAAAANA
                     VMLGAGYFNPLKGYMNKADALSVAAKLHTTAGLFWPVPVLNLTTDAAAVRGATRIALR
                     DPNVEGNPVLAIQTVEAIEEFSEAEISQMAEKIFRTLDPKHPGVATFTSLGRVAISGP
                     IEVLNFSYFQQDFPDTFRTAVEIRNEMAERGWNTVVAFQTRNPMHRAHEELCKMARDA
                     VKADGVLIHMLLGKLKAGDIPAHVRDAAIRKMVELYFPANTVMITGYGFDMLYAGPRE
                     AVLHAVFRQNCGCTHLIVGRDHAGVGSYYGAFDAQTVFDEEVPAGALGIQIFRADNTA
                     YSKKLNRVVMMRDAPDHSDEDFVQLSGTKVREMLAAGKDLPVEFARPEVARILMGYYQ
                     SEAK"
BASE COUNT          816 a         1466 c         1378 g          640 t
ORIGIN      
        1 aattctgcga tgcgctgaag caggccggtg agcagatcca gcgtgccgac gcgccgtccg
       61 atcccttcac ccgcgccgaa ggctaccgtt acctgagccg gctcgcccgc gcggggctcg
      121 aatggtacgt ggagttcaac gacccggcct tcccggtgct gtacaagccc tcgcacgaga
      181 ccatcaagat cggtgccgac aaccccgaca acatctacca gaaggccgtg gtcgacgggc
      241 gccacgacta cgtgatccgt ggcatgcgcg gcagcgtgta ttacatcagc atggcgacca
      301 gcaagggcag ctacgcggag aacttcaagc agatcgagac cggtttcctc gacagcaatt
      361 ccctgcagat cgccgccgac ggcagcttcg agatcatcct gagcgccacg ccgaagccgg
      421 gcaactggct gccgatgcat gccgattcgc agtcgctgct gatccgccag accttcatgg
      481 accgcgcgag cgagatcccg gccgagatca cgatcgagcg cgccgacgcc ggtgccacgc
      541 ccgagccgct gagcgccgag accctcgacc gcaagctgcg caccgcggcc agtttcatcg
      601 acaacaccgc gcgcctgttc gccgactgga gcgcctctta ccgcggccgg ccgaacgagt
      661 tgccgcctgc cgaccagcag ctctgccacg cgacgggcgg ggacccgaac atcttctact
      721 accacggcta cttcgagctg gccgaggacg aggcgctggt gatcgaggtc gagcgcatcc
      781 cggagtgccg cgcctggaac ctgcagaccg acaattactg gatggagtcg ctcgattacc
      841 gctatcacaa catccacctc aacaagcaca cggcgaagag ccgcgccgac ggcggcgtca
      901 cgatggtgct ggcgcaccgc gatccgggtc tcgacaactg gctggatacc gccgggcaca
      961 ggctaggcac gctgtgtttt cgctggatcg gcgcgaagga ggtcgtgcac ccgacgacgc
     1021 gcgtcgtgaa gttcgcagac ctcagggcat gacggctatg acggcagaca gattgaacga
     1081 cgagcaactt ctcgaggaag cccgtcgcga aaccgggttc gaggatttcg gcgatcccag
     1141 tttccgcgag ccgatgcgac ggttgatcga ggcgctgaat ggagaggcgc ggctcaatga
     1201 caccggtcgc gcgtatttcg ccgggcgcat ccgcagcgtg ctgatcacgc gactgcgtgc
     1261 cgaggactgg ttccggcgcc accccgagat actcgaggag gagatcgggg cgccgctcgc
     1321 gatcgtcggc ctgccgcgca ccggcaccac gatgatgcac cgcacgattt ccgcggacca
     1381 ccgcatgtat tcgccgctgt ggtacgaggt gcgctacccg gtgcccatgc ccggcaccga
     1441 cttcgcgagc ggcaacgacc cgcgcatcgc gctggccgag gccgaggtgc gcgcgatgct
     1501 cgaggcctcg cccgagctcg cttcgatcca cccgatggac gcgcgcgggc cggacgagga
     1561 catcatgctg ctcgagcagt ccttcttcag cacggtgccc gagtcctacg cgcacatgcc
     1621 ggatttcggc gtctggctcg agcggcagga ccaacgcccc ggctaccgct acctgaagcg
     1681 gttgctgcag ttcctgcagt ggcagaagaa gcagcgcggc ctcgaggccg agcgctgggt
     1741 gctcaaggca ccgcaccaca tccattttcc cgcggcgctg ttcgagacct tccccgacgt
     1801 gaaggtgctg cagacgcacc gcgacccgct gcagctgatg ccgtcctacg gcagcatgat
     1861 gtacgcgctg acgtatccgt tctcggacgc cgccgacaag gcggcgatcg cgaagcactg
     1921 gtgcgagaag tgggccacgg gcatgcatgc cacgatgcgc tttcgcgacg cgggccacga
     1981 cgaccgcttc ctcgacatgt ggtacgagga caccgtgagc agcccgctcg atgagatcca
     2041 gcgcctctac aacttcgtgg gcgtggacct gacggccgag gcccgcgccg agatggcgcg
     2101 ctggcgcgac atgaaccgcc gcgaggcgcg cccgacgcac cagtacacgc tggaagaata
     2161 cgggttcacg gaggaaggcc tgaagaacac ctttcgtgaa taccgcgagc gttatatcct
     2221 ttcgcgccgg ctgggttgac ccccgccgca agcagggctt ggccaccgcg ccgtggcgag
     2281 gacgccctgc acgccgcaac ggattgaatc tcctggattt tcctggcgct cgtgcccgtg
     2341 atgcgagcgg tggggcgcac atcagcgatc gacaacaacg gataggaaac acagcacatg
     2401 actgagcaga aggccaccaa cgtccattgg cacgaaggcg agatccagcg cgagcaccgc
     2461 cacaagctgc tcggccagca gggtgcgacc ctgtggttca ccggtctctc gggcagcggc
     2521 aagagcacgg tcgccgtggc actggaagag gcgctgtacc tgcgcggcaa gctggcctac
     2581 cgcctcgacg gcgacaacat ccgcctcggc atcaacaaga acctgggctt cagcgccgat
     2641 gatcgcaagg aaaacatccg ccgtatcggc gaggtggcga agctgttcgt ggactgcggc
     2701 gtgatcgtgc tgaccagctt catcagcccc taccgctcgg accgcgacat cgtgcgccag
     2761 ctgcacgcgg cctcggacat gccgttcatc gaggtgcacg tggactgcgc gctggccgaa
     2821 gccgagaagc gcgaccccaa gggcctgtac aagaaggccc gcgccggcga gatcaagaac
     2881 ttcaccggca tcgacgatcc ctacgaggcc cccgaggccc ccgagatcca cctgcatacc
     2941 gaccgcatga ccctgcagca ggaagtcgac atgatcctgc acgagctcga gaagcgcggc
     3001 atcctttcgg cctgacaacg gagtaaagac acgatgatca aaccccacgg ttcggacgcc
     3061 ctcgatccgc gcttcgtcta cgacaccgag cgccaccacg cgctcatgaa ggaagccgag
     3121 tcgctgccct cgctgctgct gaattccgcc gcggcggcca acgcggtgat gctgggcgcg
     3181 ggctacttca acccgctgaa gggctacatg aacaaggccg atgcgctgag cgtggccgcg
     3241 aagctgcaca ccacggcggg cctgttctgg ccggtgccgg tgctgaacct caccaccgat
     3301 gccgccgccg tccgtggcgc gacgcgcatc gcgctgcgcg acccgaacgt cgagggcaac
     3361 ccggtgctcg ccatccagac ggtggaggcc atcgaggagt tctcggaggc cgagatcagc
     3421 cagatggcgg agaagatctt ccgcacgctg gatccgaagc acccgggcgt ggccacgttc
     3481 accagcctgg gccgggtggc gatcagcggc ccgatcgagg tgctgaactt cagctatttc
     3541 cagcaggact tcccggacac cttccgcacc gcggtcgaga tccgcaacga gatggccgag
     3601 cgcggctgga acaccgtggt ggccttccag acccgcaacc ccatgcaccg cgcccacgag
     3661 gagctgtgca agatggcgcg cgacgcggtc aaagccgacg gtgtgctgat ccacatgctg
     3721 ctgggcaagc tgaaggcggg cgacatcccc gcgcacgtgc gcgacgcggc gatccgcaag
     3781 atggtggagc tgtacttccc cgccaatacc gtgatgatca ccggctacgg tttcgacatg
     3841 ctctacgccg gtccgcgcga ggcggtgctg cacgcggtgt tccgccagaa ctgcggctgc
     3901 acgcacctga tcgtcggccg tgaccacgcc ggtgtcggca gctactacgg cgccttcgac
     3961 gcacagaccg tgttcgacga ggaagtgccc gcgggcgcgc tcgggatcca gatcttccgc
     4021 gccgacaaca cggcctattc gaagaagctg aatcgcgtgg tgatgatgcg cgacgcgccg
     4081 gaccacagcg acgaggactt cgtgcagctc tccggcacca aggtgcgcga gatgctggcc
     4141 gcgggcaagg acctgccggt ggagttcgcc cgccccgagg tggcccgcat cctgatgggc
     4201 tactaccaga gcgaagccaa gtaacgatcg cccccgtccc gcccgcacgt cgcgggcggg
     4261 atccacgttg cacatgaacc tgctaagccg ttgatccggc
//
LOCUS       DAORUQ010000232         4295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26123_length_4295_cov_5.311557, whole genome shotgun sequence.
ACCESSION   DAORUQ010000232 DAORUQ010000000
VERSION     DAORUQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26123_length_4295_cov_5.311557"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1281)
                     /locus_tag="PLW13_14565"
     CDS             complement(142..1281)
                     /locus_tag="PLW13_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY71653.1"
                     /translation="MALTLNEEQRLLRETAREFLGANAPVGALRALRDARDPLGYSRE
                     LWTRMAGLGWAGIIIPEEFGGLGFGFAGLGAVLEETGRTLTASPLFASCVLGASAVLL
                     AGSRAQKEAVLPRVASGELCLALALEESHRHDPCGVHLEARRHGEGFRLNGAKQFVLD
                     GHSADQLVVVARSRGTAGATDGISLFLVDANAPGITRKRSLLMDSRNAARIEFANVDV
                     GADALLGTEGEAWPALEQVLDRGRAAIAAEMLGGVQETFERTVDYLKQREQFGTKIGT
                     FQALQHRAAHMFSEIELLKSVVLDACANVDANPALLPLSASLAKAKANDTFELVSNEA
                     VQMHGGIGTTDELDIGLFLKRSRVAMQILGDARFHRARYATLRGF"
     gene            complement(1306..2499)
                     /locus_tag="PLW13_14570"
     CDS             complement(1306..2499)
                     /locus_tag="PLW13_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010656629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71654.1"
                     /translation="MEELEQFRVEVREWLEANCPASQRLPITPEQQYWGGRNGSFASD
                     DARVWFERMLAKGWTVPEWPREYGGAGLDAQQARILKKEMTRLGCRTPLYGLGIWMLG
                     PALLEYGNEEQKRVHLRAIAEGRLRWCQGYSEPGAGSDLASLQTRAEDRGDHFLVNGS
                     KIWTSGADKADWIFCLVRTDPQARKQQGISFLLIDMATPGVSVSPIELISGSSEFCQT
                     FFDNVRVPKDNLVGELNAGWSVAKALLKHERKLMSEIGTETASAAYTPIECARLYLPL
                     EDGRVADAVMRDRLAHHEMQMHAVGLTQMRIIEETRAGTGSGLTALVMKYCGTEEEKR
                     KQELMVAMMGSQGLGWEGDDFKAAEIDTVRQCLSTKALSIAGGTSEVQLNIIAKRALD
                     LPGVT"
     gene            2701..3450
                     /locus_tag="PLW13_14575"
     CDS             2701..3450
                     /locus_tag="PLW13_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71655.1"
                     /translation="MKRFEGKVALITGAGSGIGRAAALRLASEGARVFCVDLSAPDVE
                     ETKAQIEAAGGRAEAHACDVSDEAAVRACVQACVDVCGRIDHLSNMAGILRFDHFHEI
                     ALADFRRIVEVNLYGTFLMCREAIPHLLATKGNIVNAASTSALAGLPWGGAYSASKGA
                     VLAMTRTLAVEYGRAGLRANCVCPGDIATPMSKAPRMPKGADFSWMARCSSLNGPMGP
                     EVVAGVIAMLASGDGAHINGEDIRVDGATLS"
     gene            3465..>4295
                     /locus_tag="PLW13_14580"
     CDS             3465..>4295
                     /locus_tag="PLW13_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY71656.1"
                     /translation="MHALIEGLREARAFPHAIGPVRVVETHISWVLLTGDFAYKIKKP
                     LDLGFLDYSTLALRKRFCEEELRVNRRYAPQIYLDVVAISASAQGPQIGGEGTVIEYA
                     VRMRQFPDDALLADVVARTDPGTAVWRRLGADIAAIHDALPVAGPGHEDGRGTPGKVR
                     DAINQNFRQVRPYLREGADIAQLNRLEEQAWQDFHRHERLLWSRYASGLVRECHGDLH
                     LGNIVLLAGHALAFDAIEFNPALSWIDVMNEVAFLVMDCESRGHGAAGFVALNAWLER
                     T"
BASE COUNT          648 a         1450 c         1487 g          710 t
ORIGIN      
        1 aaatctgcga tttcgggccg ggccccggag ggggatttac ggcgtgtccg cggcagcgat
       61 gggtgctcgg gcgccgatgg ctcgcgatac gcgggattgt cgggctcggg cagcggcccg
      121 cgacgctcca gacagagtgc ttcagaagcc ccgcagcgtc gcataccgcg cgcgatggaa
      181 gcgcgcgtcg ccgaggatct gcatcgccac gcgcgagcgc ttcaggaaca gcccgatgtc
      241 gagctcgtcg gtggtgccga tgccgccgtg catctgcacc gcctcgttgc tcacgagctc
      301 gaaggtgtcg ttggccttcg ccttggcgag gctggccgag agcggcagca gcgcagggtt
      361 cgcatccacg ttggcgcagg catcgagcac cacgctcttc agcagctcga tctccgagaa
      421 catgtgcgcg gcgcggtgct gcagcgcctg gaaggtgccg atcttcgtgc cgaactgctc
      481 gcgctgcttg aggtagtcga cggtgcgctc gaaggtttcc tgcacgccgc ccagcatttc
      541 ggccgcgatc gccgcgcggc cgcgatcgag cacctgctcg agcgccggcc aggcctcgcc
      601 ctcggtgccg agcagcgcat cggcgcccac gtccacgttg gcgaactcga tgcgcgcggc
      661 attgcggctg tccatcagca gcgaacgctt gcgcgtgatg ccgggcgcgt tggcgtccac
      721 caggaacagg ctgatgccgt ccgtcgcgcc ggccgtgccg cgactgcgcg cgaccaccac
      781 cagctggtcg gcgctgtggc cgtcgagcac gaactgcttg gcgccgttca ggcggaagcc
      841 ctcgccgtgg cgcctcgcct cgaggtgcac gccgcagggg tcgtggcgat gcgattcctc
      901 cagcgccagc gcgaggcaca actcgccgct tgcgacgcgc ggcagcaccg cttccttctg
      961 tgcccgggaa cctgccagca gcacggcgga agcgccgagc acgcagctcg cgaacagcgg
     1021 cgaggcggtg agcgtgcgac cggtttcctc gagcaccgcg ccgagtccgg cgaagccgaa
     1081 gccgagtccg ccgaactcct ccgggatgat gatgccggcc cagccgagcc cggccatccg
     1141 tgtccacagc tcgcgcgaat agccgagcgg gtcgcgcgcg tcgcgcagcg cgcgcagcgc
     1201 ccctaccggc gcgttggcgc cgaggaattc ccgggccgtt tcgcgcagca ggcgctgctc
     1261 ctcgttcagt gtgagtgcca tgtgatcggt tccggcgcga atgcttcagg taacgccggg
     1321 aaggtccagg gcgcgcttgg cgatgatgtt gagctggacc tccgaggtgc cgccggcaat
     1381 gctgagggct ttcgtggaga ggcattggcg caccgtgtcg atctcggcgg ctttgaagtc
     1441 atcgccttcc cagccaagcc cctggctgcc catcatcgcc accatcagct cctgtttgcg
     1501 cttctcttcc tcggtgccgc agtacttcat gaccagcgcc gtgagcccgc tgccggtgcc
     1561 ggcgcgcgtc tcctcgatga tgcgcatttg cgtcagcccg accgcgtgca tctgcatctc
     1621 gtggtgcgcg agcctatcgc gcatcactgc gtcggccacg cgtccgtcct cgagcggtag
     1681 gtagaggcgg gcgcactcga tcggcgtgta cgcggcgctc gcggtctcgg tgccgatctc
     1741 ggacatgagc ttgcgctcgt gcttgagcag cgctttcgcc acgctccagc cggcgttgag
     1801 ctcgccgacc aggttgtcct tcggcacgcg cacattgtcg aagaaggtct ggcagaactc
     1861 ggaactgccg ctgatcagtt cgatcggcga gacggagacg cccggcgtgg ccatgtcgat
     1921 cagcaggaag ctgatgccct gctgcttgcg cgcctgcggg tcggtgcgca ccaggcagaa
     1981 gatccagtcg gccttgtcgg cacccgaggt ccagatcttg gagccgttca cgagaaagtg
     2041 atcgccgcgg tcttccgcgc gcgtctgcag gctcgcgaga tcggagccgg cgcccggttc
     2101 gctgtagccc tggcaccagc gcaggcgtcc ctcggcgatc gcacgcaggt gcacgcgctt
     2161 ctgctcctcg ttgccgtatt ccagcagcgc cgggccgagc atccagatgc ccagcccgta
     2221 gagcggcgtg cggcaaccca ggcgtgtcat ctccttcttg aggatgcgcg cctgctgcgc
     2281 gtcgagcccc gcgccgccgt attcgcgcgg ccactcgggc acggtccagc ccttggccag
     2341 catgcgctcg aaccacacgc gcgcgtcatc ggaggcgaag ctgccattgc gcccacccca
     2401 gtactgctgc tcgggcgtga tcgggaggcg ctgcgaggcc gggcagttgg cctccagcca
     2461 ttcccggacc tcgacgcgaa actgttccag ttcttccata ctgcatcctt gggcagaaca
     2521 gccgattgcg tagaagcacg atgttaaggc ctggggtcgg atgcgcaatg acaatttggg
     2581 tcggcgccaa ttaacaataa cggtcagcgc caactgcctg actcgcatgg ctttttcggt
     2641 gcaatccatt actatgcaaa aaattaccgg aacgtagcgg caatcgccgg gagtgcaggc
     2701 atgaagagat tcgagggcaa ggttgcgctg atcaccggcg cgggttcggg catcgggcgc
     2761 gcggcggcgc tgcgcctggc gagcgagggt gcgcgcgtgt tttgcgtgga cctgtccgcg
     2821 ccggacgtcg aggagacgaa ggcacagatc gaggccgcgg gcgggcgcgc ggaggcgcac
     2881 gcctgcgacg tcagcgacga ggccgcggtg cgcgcctgcg tgcaggcctg cgtcgatgtc
     2941 tgcgggcgca tcgaccacct gagcaacatg gccggcatat tgcgcttcga tcacttccac
     3001 gagatcgcgc tcgccgactt ccgccgcatc gtcgaggtca acctctacgg tacgttcctg
     3061 atgtgccgcg aggcgatccc gcacctgctc gcgacgaagg gcaacatcgt caacgcggca
     3121 tcgacctccg cgctggccgg cctgccgtgg ggcggcgcct attcggcatc caagggcgcg
     3181 gtgctcgcga tgacgcgcac cctcgcggtg gagtacggtc gtgccgggct gcgcgccaac
     3241 tgcgtgtgcc ccggcgatat cgcgacaccc atgtccaagg cgccgcgcat gcccaagggc
     3301 gccgatttca gctggatggc gcgctgcagc tcgctcaacg gaccgatggg gccggaagtc
     3361 gtggccggcg tgatcgcgat gctcgcttcc ggggacggcg cgcacatcaa cggcgaggac
     3421 atccgcgtcg acggcgcgac gctgtcctga ggcgccggcc gatcatgcac gcgctgatcg
     3481 aggggctgcg cgaggcgcgc gcctttccgc atgccatcgg gccggtgcgc gtcgtcgaaa
     3541 cccacatttc ctgggtgctg ctgaccgggg atttcgccta caagatcaag aagccgctgg
     3601 atctgggctt cctggactac tccacgctgg cattgcgcaa gcgcttctgc gaggaggagt
     3661 tgcgcgtgaa ccggcgctac gcgccgcaga tctacctcga cgtggtggcg atcagcgcct
     3721 cggcgcaggg cccgcagatc ggcggcgagg gcacggtcat cgaatacgca gtgcgcatgc
     3781 gccagttccc cgacgatgcg ctgctcgcgg acgtggtcgc acgcaccgac ccgggcaccg
     3841 cggtgtggcg ccggctgggt gcggacatcg cggcgatcca cgacgcgctg ccggtggccg
     3901 gccccgggca cgaggatggg cgcggcaccc cggggaaggt gcgcgacgcg atcaaccaga
     3961 acttccgcca ggtgcgtccg tacctgcgcg agggtgccga catcgcgcag ctgaaccgtc
     4021 tcgaggagca ggcgtggcag gacttccatc gccacgagcg cctgctgtgg tcgcgctacg
     4081 caagcggact ggtgcgcgag tgccacggcg acctgcatct gggcaatatc gtgctgctcg
     4141 cggggcacgc actcgccttc gacgccatcg agttcaaccc ggcgctcagc tggatcgacg
     4201 tgatgaacga ggtggccttc ctggtgatgg actgcgagag ccgcggccac ggcgcggcgg
     4261 gattcgtcgc gctgaacgcc tggctcgagc gcacg
//
LOCUS       DAORUQ010000233         4272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26347_length_4272_cov_6.371354, whole genome shotgun sequence.
ACCESSION   DAORUQ010000233 DAORUQ010000000
VERSION     DAORUQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26347_length_4272_cov_6.371354"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLW13_14585"
     CDS             <1..314
                     /locus_tag="PLW13_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HQY71657.1"
                     /translation="FQRELGAMGYKYQFITLAGIHSMWYNMFELAYGYARNGMTSYVE
                     LQEKEFAAANRGYTFVSHQQEVGTGYFDQVTTCIQGGTSSVTALTGSTEEEQFTVVSS
                     H"
     gene            476..961
                     /gene="rraA"
                     /locus_tag="PLW13_14590"
     CDS             476..961
                     /gene="rraA"
                     /locus_tag="PLW13_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HQY71658.1"
                     /translation="MSLSTCDLCDQHPEAVRVADPLFRDFGGRQAFGGRIVTIKCHED
                     NSLVRDLVAEPGHGCVLVIDGGGSLRRSLLGDQLAERAVANGWEGIVVFGAVRDVEAI
                     AHMNLGVKALAAIPLKTDKRGIGERDIPVRFAGVDFAPGEYLYADANGIIVAATALAR
                     A"
     gene            974..2122
                     /locus_tag="PLW13_14595"
     CDS             974..2122
                     /locus_tag="PLW13_14595"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY71659.1"
                     /translation="MKTSVLGEIGERRFLREYWQRKPLLIRQALPGFQSPLTPDELAG
                     LALEEEVESRLVLERGRKGPWELRNGPFTAEDFAKLPKTRWSLLVQAVDQWVPEVHAL
                     RAHFDFLPAWRFDDLMISYAPDQGGVGPHFDHYDVFLLQATGRRRWRIGPACTDATPV
                     RTDTSLRILEHFETLEEFVLEPGDMLYIPPGVSHWGIAEGECMTFSIGFRAPSHAEIL
                     VDIANGAAQRLDEGLRYMDPPLGSDVQEGEIPPAALRRVQRVLQEMAADSELVREWFG
                     RYMTQRKYADLDITPEALPDDLRAWLRAGGVLERHPASRFAWSGAEAPFMLFVDGEAL
                     PCDDPALARLLCSRAALDARALKPALARQPGRALLEELVARGALYCED"
     gene            complement(2107..3876)
                     /gene="ggt"
                     /locus_tag="PLW13_14600"
     CDS             complement(2107..3876)
                     /gene="ggt"
                     /locus_tag="PLW13_14600"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQY71660.1"
                     /translation="MRVIVSLVLSLFVALAAPGLHAEAKQAPRVPIIGYDARFHPVPS
                     REGMVVSSEALASEVGARILAAGGNAIDAAVATGFALAVTYPQAGNIGGGGFMLVHLA
                     NEGRTVAIDYREMAPRAARRDMFLDKQGEVVPRSSQLTHLASGVPGSVAGLLHALERY
                     GRLDRATVMAPAIELAENGFPVTYTLSNTLASPWGQRLLANEAAKSYLFKPDGSVYQP
                     GEILRQRDLAWTLRQIAERGAAGFYEGPVADRLVAEMKRGGGLITYRDLKRYKAVERE
                     PVRGSFKGYEIVSMPPPSSGGVHMVQMLNVLEGFPLREMQLNSAAYIHVLAETMKRAY
                     ADRSKHLGDPDFHRVPVAELTSRTYAAQLRAAIDPERATPSAAIAPTTQFPRESTQTT
                     HYSVIDAEGNVVSNTYTLNFSFGSGIAVPGAGFFLNNEMDDFSVKPGVPNVFGLIGDE
                     ANAVAPRKRPLSSMTPTIVLKDGKPFLATGAPGGSRIITVVLQIILNTLVFDMNIADA
                     SAQSRVHHQWLPDELYLEPGISADTEALLRAMGHAIGAPGPMFGNAQSLVFRDGVMYG
                     ASDTRRPGGAVATVEEVRAQSSQ"
BASE COUNT          637 a         1433 c         1502 g          700 t
ORIGIN      
        1 agttccagcg cgagctcggc gcgatgggct acaagtatca gttcatcact ttggccggca
       61 tccacagcat gtggtacaac atgttcgagc tcgcctacgg ctatgcgcgc aacggcatga
      121 cctcctacgt cgagctgcag gagaaggagt tcgccgccgc gaaccgcggt tacaccttcg
      181 tcagccacca gcaggaagtg ggtaccggct atttcgacca ggtcactacc tgcatccagg
      241 gtggcacctc ctcggtcacc gcgctcaccg gctccacgga agaagagcag ttcacggtgg
      301 tgtcgagtca ttgagataga gagattgtcg ggctgaagcc cgaccgacaa ggggccgcga
      361 ggcccctttt tcgttgtggc ggcggctcac cggcggcgcg ggcgcgcggc tataatcgcg
      421 ccgcatcccg ggccgctggc gagccgcggc acaccccatc atcaggagca atcccatgag
      481 cctcagtacc tgcgacctct gtgaccagca tcccgaggcg gtgcgcgtgg cagacccgct
      541 gttccgcgac ttcggcgggc gacaagcctt cggcgggcgc atcgtcacga tcaagtgtca
      601 cgaggacaac tcgctggtgc gcgacctcgt cgccgagccc ggacacggtt gcgtactggt
      661 catcgacggc ggcggctcgc tgcgccgttc gctgctcggc gaccagctcg ccgaaagggc
      721 ggtcgccaac ggctgggaag gcatcgtggt gttcggcgcg gtgcgtgacg tcgaggcgat
      781 cgcgcacatg aacctcggcg tgaaggcgct cgccgcgata ccgctgaaga ccgacaagcg
      841 cggcatcggc gagcgcgaca ttcccgtgcg cttcgccggc gtcgatttcg cgcccggcga
      901 atacctgtac gccgatgcca acggcatcat cgttgctgcc accgcgctcg cgcgcgcctg
      961 aggagccggg accatgaaaa ccagcgtgct gggcgagatc ggtgagcgcc gtttcctgcg
     1021 cgaatactgg cagcgcaagc cgctgctgat ccgccaggcc ctgcccggat tccagtcgcc
     1081 gctcacaccc gacgagctgg cggggctcgc gctcgaagag gaggtggagt cgcgcctggt
     1141 gctggagcgt ggcaggaagg ggccgtggga actgcgcaac ggaccgttca cggcggagga
     1201 tttcgcgaag ctgccgaaga cgcgctggtc gctgctggtg caggcggtgg accagtgggt
     1261 gcccgaggtg catgcgctgc gcgcgcactt cgatttcctg cccgcgtggc gcttcgacga
     1321 cctgatgatc agttacgcac cggaccaggg cggcgtgggg ccgcacttcg atcattacga
     1381 cgtgttcctg ctgcaggcga cggggcgccg gcgctggcgc atcgggcccg cgtgcacgga
     1441 tgccacgccg gtgcgcaccg ataccagcct gcgcatcctc gagcacttcg agacgctgga
     1501 ggagtttgtg ctggagccgg gcgacatgct ctacatcccg cccggtgtgt cgcactgggg
     1561 catcgccgaa ggcgaatgca tgacgttctc catcgggttc cgcgcgccga gccatgcgga
     1621 gatcctcgtg gacatcgcca acggcgcggc ccagcgcctc gacgagggac tgcgctacat
     1681 ggatccgccg ctcggctccg acgtgcagga gggcgagatt ccgccggccg cgctgcggcg
     1741 cgtgcaacgg gtgttgcagg agatggctgc ggactcggaa ctggtgcgcg agtggttcgg
     1801 ccgctacatg acgcagcgca agtacgccga cctggacatc acgcccgagg cgcttcccga
     1861 tgacctgcgt gcctggctgc gcgcgggcgg cgtgctcgag cgccatcccg cctcgcgttt
     1921 cgcgtggagc ggtgcggagg ctcccttcat gctgttcgtc gacggcgagg cgctgccctg
     1981 cgacgacccg gcgctcgcgc ggctgctgtg ctcgcgcgcg gcgctggatg cgcgcgcgct
     2041 gaagccggca ctggcgcgcc agcctggtcg tgcgctgttg gaggaactcg tggcgcgcgg
     2101 cgcgctctac tgcgaggact gagcgcgcac ttcctccacc gtggcgaccg caccgccggg
     2161 tcggcgcgtg tcgctcgccc cgtacatcac gccatcgcgg aacaccagcg actgcgcatt
     2221 gccgaacatc ggacccggcg cgccgatcgc gtggcccatc gcgcgcagca gtgcttcggt
     2281 gtcggcgctg atgccaggct ccaggtagag ttcgtccggc agccactggt ggtggacgcg
     2341 cgattgcgcg ctggcatcgg cgatgttcat gtcgaagacc agcgtgttca ggatgatctg
     2401 aagtaccacg gtgatgatgc gcgagccgcc cggcgcgccg gtcgcgagga agggcttgcc
     2461 gtccttcagc acgatggtcg gcgtcatcga ggacagcggg cgcttgcgcg gtgcgactgc
     2521 gttggcctcg tccccgatca gcccgaacac gttgggcacg cccggcttca cggagaaatc
     2581 gtccatctcg ttgttcagga agaagccggc gccgggcacc gcgatgccgc tgccgaaaga
     2641 gaagttcagc gtgtaggtgt tggacaccac gttgccctcg gcgtcgatca ccgagtagtg
     2701 cgtggtctgc gtgctctcgc gcgggaattg cgtggtgggc gcgattgcgg ccgagggcgt
     2761 ggcgcgctcg ggatcgattg ccgcgcgcag ctgtgccgcg taggtgcggg aggtcagttc
     2821 cgccaccggt acgcggtgga aatccgggtc gccaaggtgc ttgctgcggt cggcataggc
     2881 gcgcttcatg gtttccgcga gcacgtggat gtaggcggcg ctgttcagct gcatctcgcg
     2941 cagcggaaag ccttccagca cgttcagcat ctgcaccatg tgtacgccgc cggaggaggg
     3001 cggcggcatc gacacgatct cgtagccctt gaagctgccg cgcaccggct cgcgctccac
     3061 ggccttgtag cgcttcaggt cgcggtacgt gatcagtccg ccgccgcgct tcatctcggc
     3121 caccagccgg tcggccaccg gcccctcata gaaacccgcc gcgccgcgct cggcgatctg
     3181 gcgcagcgtc catgcgaggt cgcgctggcg caggatctcg ccgggctggt ataccgagcc
     3241 gtcgggcttg aacaggtagg acttcgcggc ctcgttcgcc agcagtcgct ggccccaggg
     3301 gctcgcgagc gtgttcgaca gcgtatacgt gaccgggaag ccgttctccg cgagctcgat
     3361 cgcgggcgcc atcacggtgg cgcggtcgag gcggccatag cgttccagtg cgtgcagcag
     3421 gccggcgacc gagcccggca cgccggaggc gaggtgcgtg agctgcgagc tgcgcggcac
     3481 gacttcaccc tgcttgtcca ggaacatgtc gcggcgtgcg gcgcgcggcg ccatctcgcg
     3541 gtagtcgatg gccaccgtgc gcccttcgtt cgcgaggtgc accaacatga agccgccgcc
     3601 gccgatgttg ccggcctgcg ggtaggtgac ggcgagcgcg aagcccgtgg ccaccgctgc
     3661 atcgatcgcg ttgccgccgg cggcgagtat gcgcgcaccg acctcgctgg cgagcgcctc
     3721 gctgctcacc accatgccct cgcgcgaggg caccgggtgg aaacgcgcgt cgtagccgat
     3781 gatgggtacg cgcggcgcct gtttcgcctc cgcatgcaag ccgggcgcgg cgagcgcaac
     3841 gaacaggctg agtactagcg aaacgatgac gcgcatgcgg gtatccggtg agttggcttc
     3901 aatcggcggg cattctaacc tgtgcgctcc gatccggcgc tccgatccgg cgctccgatc
     3961 cggcgctccg atccggcgct ccgatccggc gcgccgaggg gcgggcccgt gccgcgtgcg
     4021 gccgccgccg gtacgtccat gtaccggcgg ctacgcgtgt tgccggaatc gctcccggcg
     4081 attccggcat ccacaggtcc gctcgcggca cggacccgcc cctcgtcacg cctaccgctg
     4141 gcagtgatcc ggatggaggt tggaatcggc ggctgtttcg cgcgcatcac gagtcatcgg
     4201 cgcccgagca cccatcggtg ccgcggacac gccgtgaatc cccctccggg gcccggcccg
     4261 aaatcgcaga tt
//
LOCUS       DAORUQ010000234         4257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26493_length_4257_cov_4.732984, whole genome shotgun sequence.
ACCESSION   DAORUQ010000234 DAORUQ010000000
VERSION     DAORUQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26493_length_4257_cov_4.732984"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLW13_14605"
     CDS             <1..1185
                     /locus_tag="PLW13_14605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY71661.1"
                     /translation="AGLAGSSLALHLAEAGVDVVVLEARQPGWGASGRNAGHVLPTLK
                     DVALFERFPDGGKAFLDLFREHHTITFDLARKHGIACDAVQSGYLHATTRESVFDKLK
                     ATSVYWKQEQGQQVEWLEGAEVEALTGSKYFGYGVLYRSGGRVNPYLFTNGLIAAATA
                     RGARVFGESEAVALSRSGNRWRVATAQGAVVADRVVFCTNAYATAIVPQFADNFYPLT
                     AYAVSTKPLPRAALDIVMPSRATLSEEPVDLNPFVIDGHGRIITSSIPSASRPDDAAW
                     HFANHLAWIHRTWPETRGMAIELEDYWTGRVALRDVEFPGMFQLEPGVFGLMHFNAWG
                     NLMAPLMGMALAGAMAGDRMDQLPFPVERPVPVANPGKQDLIIRKLMIPAARMGQRIG
                     II"
     gene            complement(1213..1977)
                     /locus_tag="PLW13_14610"
     CDS             complement(1213..1977)
                     /locus_tag="PLW13_14610"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY71662.1"
                     /translation="MYQVNLEGKVALVTGGSRGLGRAMVLGLARAGADVVIASRKLAG
                     CEEVAAEVQSMGRQALAVSAHAGQMESLDALLEATYQRFGRLDILINNAGTNPVTAGL
                     SDLTPELFQKVFEVNTKGPWYLASRAAPRMAQHGGGSIINVISVGGLKPPAYQGFYAA
                     TKAALHALTKVMAAEWAQSGIRVNALAPGSYHSDMFDKSAAVLPGFTEGSMAATLQKR
                     IAETDEILGPVMYLASDMSSYTTGTTLIADGGYLVL"
     gene            complement(2043..>2312)
                     /locus_tag="PLW13_14615"
     CDS             complement(2043..>2312)
                     /locus_tag="PLW13_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71663.1"
                     /translation="EMSILKLVAGKGILDIASFALELAGPEGVLGHQDLGSEWGFIQR
                     LWLSAPGIRLAGGSDEIMRNIIAERVLGLPGEVRTDRKTPFRELL"
     assembly_gap    2313..2412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2413..3072)
                     /locus_tag="PLW13_14620"
     CDS             complement(<2413..3072)
                     /locus_tag="PLW13_14620"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71664.1"
                     /translation="MDFRDSPEEAAYRARVRSWLEANATPRRSAEDRFEAGMSDAEHI
                     AAARAWQRRKAAAGYAAITWPTEWGGPGGTPIEQVIYSQEEARYLVPTQVFVNSIGQC
                     LPTVAMWALPELRERYVPQGLRGDAIWCQLFSEPAAGSDTGGIRTRATREGDEWVING
                     QKVWTSGAHFSDYGILLARSDWDKPKQEGLTMFIVDMKAAGIEIRPIHQMSDEYEFNE
                     VF"
     gene            complement(3085..4170)
                     /locus_tag="PLW13_14625"
     CDS             complement(3085..4170)
                     /locus_tag="PLW13_14625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71665.1"
                     /translation="MNFDFSDDQRLLQQEVRRMLADTSTSATVRTVLEGKEACCAQVW
                     RNLVGMGAAGAAIPEAYGGAGFGYLELCLVAEEIGRHLAAVPVSSSFYLAAEAILHGG
                     SEQQKQQWLPRIAAGELVATAALGSGESYRGLPPLAFDGATLNGTAGVVPDGAIAGLA
                     VLRAGTELVLVDLAGAGVSRRPLSSLDPTRPVAQLTFAGTPAQKLPADGAALARRVLN
                     GAAVLLAFEQVGGAARILELSRDYSLERKAFGRQIGGFQALKHKMADIYTRNELARVH
                     AYYGAWALSTDAPELPLAAAAARVAATTAFNFAAEEGIEIHGGIGFTWEMDCHLYLRR
                     ARWLAQSIGSEHLWRSQLADELVKEAA"
BASE COUNT          610 a         1439 c         1436 g          672 t
ORIGIN      
        1 gcgggactgg ccggcagctc gctggcgctg cacctcgccg aggccggcgt cgacgtggtc
       61 gtgctggagg cgcgacagcc gggctggggc gcgtccgggc gcaatgccgg gcacgttttg
      121 cctacgctga aggacgtcgc gttgttcgag cgctttcccg atggcggcaa ggctttcctg
      181 gacctgttcc gcgagcacca caccatcacc ttcgatcttg cgcgcaagca cggcatcgcc
      241 tgcgatgcgg tgcagtcggg ctacctgcac gccaccactc gcgagagcgt gttcgacaag
      301 ctgaaagcga cgtccgttta ctggaagcag gagcaggggc agcaggtgga gtggctcgag
      361 ggcgccgagg tcgaagcgct gacgggctcg aaatatttcg gctacggcgt gctgtaccgc
      421 tcgggcgggc gggtgaatcc ctacctgttc accaacggcc tgatcgcggc cgcgacggca
      481 cgcggcgcgc gtgtcttcgg cgagagcgaa gccgttgcgt tgagcagatc gggcaaccgc
      541 tggcgtgtgg cgacggcgca gggcgctgtc gttgccgatc gcgtcgtgtt ctgcaccaac
      601 gcctatgcga ccgcgatcgt tccgcagttc gccgacaatt tctatccgct cacggcctac
      661 gcggtttcca cgaagccgtt gccaagggcc gcgctcgaca tcgtgatgcc gagccgagca
      721 acgctgtccg aggagcccgt ggacctgaat cccttcgtga tcgacggaca cggcaggatc
      781 atcacctcga gcatccccag cgcctcgcgc ccggacgacg ccgcgtggca tttcgcgaac
      841 cacctggcct ggattcaccg cacatggccc gaaacccggg gcatggcgat cgagctggag
      901 gattactgga ccggccgggt ggcgctgcgc gacgtggagt tccccgggat gttccagctc
      961 gagcccggcg tgttcggcct gatgcacttc aatgcctggg gcaacctgat ggccccgctg
     1021 atgggcatgg cgctggccgg tgccatggcc ggcgaccgca tggaccagct gccgttcccg
     1081 gtggagaggc cagtgcccgt cgccaacccc ggcaagcagg acctgatcat ccgcaagctg
     1141 atgattcccg ccgccaggat ggggcagcgg atcgggatca tctgagcggg gaccgccgct
     1201 gcgccccgcg gctcacagca ccaggtagcc gccgtcggcg atcagcgtgg tgccggtggt
     1261 gtagctcgac atatcgctcg cgaggtacat caccgggccg aggatctcgt cggtctcggc
     1321 gatgcgtttc tgcagcgtgg ccgccatgga gccctcggtg aagccgggca gcaccgcggc
     1381 cgacttgtcg aacatgtccg agtggtagct gccgggcgcg agcgcattga cgcggatgcc
     1441 cgattgggcc cactcggcgg ccatgacctt ggtcagcgcg tgcagcgcgg ccttggtggc
     1501 ggcgtagaag ccctggtatg cgggcggctt gagcccgccg accgagatca cgttgatgat
     1561 cgatccgccg ccgtgctgag ccatgcgtgg cgcggcgcgc gaggcgagat accacggccc
     1621 cttggtgttc acctcgaaca ccttctggaa cagttccggc gtcaggtcgg agaggcccgc
     1681 ggtgacggga ttcgtgccgg cgttgttgat caggatgtcc aggcggccga agcgctggta
     1741 ggtcgcctcc agcagcgcgt ccaggctctc catctgcccg gcatgggccg acacggcgag
     1801 cgcctggcgg cccatggact gaacttcggc cgccacttcc tcgcagccgg ccagcttgcg
     1861 gctcgcgatc accacatcgg cgccggcacg ggcgagcccg agcaccatcg cgcgaccgag
     1921 gccgcggctg ccgccggtga ccagcgccac cttgccttcg agattgacct gatacacggg
     1981 aaccctccgt tgatggatcg atgttcggcg ccgcgcgcga tgttcgcggc catgggccgc
     2041 tcctacagca gctcgcggaa cggtgtcttg cggtcggtgc gcacttcgcc gggcagcccc
     2101 agtacccgct cggcgatgat gttgcgcatg atctcgtcgg agccgccggc aaggcggatg
     2161 cccggcgccg acagccacag ccgctggatg aagccccatt cgctgccgag atcctggtgg
     2221 ccgagcacgc cctcggggcc ggcgagctcc agcgcgaagc tggcgatatc caggatgccc
     2281 ttgccggcga ccagcttgag tatcgacatc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nngaagacct cgttgaactc gtactcgtcc gacatctggt gaatgggccg
     2461 gatctcgatg ccggccgcct tcatgtccac gatgaacatg gtcaggcctt cctgcttggg
     2521 cttgtcccag tcgctgcgcg ccagcaggat cccgtagtcg ctgaagtggg cgcccgaggt
     2581 ccacaccttc tgcccgttga tcacccactc gtcgccctcg cgcgtggcac gcgtgcggat
     2641 gccgccggtg tccgagcccg ccgcgggttc cgagaagagc tggcaccaga tcgcatcgcc
     2701 cctgagtccc tgcgggacgt agcgctcgcg cagctccggc agggcccaca tggcgaccgt
     2761 gggcaggcac tggccgatgc tgttcacgaa gacctgggtc ggcaccaggt agcgcgcttc
     2821 ctcctggctg tagatcacct gctcgatcgg cgtgccgccg gggccgcccc actcggtcgg
     2881 ccaggtgatc gcggcatagc cggcggcggc cttgcgccgc tgccaggcgc gcgccgccgc
     2941 gatgtgctcg gcatcgctca tgccggcttc gaaccggtcc tccgcgctgc gccgcggcgt
     3001 tgcgttggcc tcgagccagc tgcgcacccg cgcgcggtag gctgcttctt ccgggctgtc
     3061 cctgaaatcc atgttgcgtt ccgcttacgc ggcctccttc acgagttcgt ccgccagctg
     3121 gctgcgccag aggtgctcac tgcctatcga ctgggcaagc cagcgcgcgc ggcgcaggta
     3181 gaggtggcag tccatctccc aggtgaagcc gatgccgccg tggatctcga tgccttcctc
     3241 tgccgcgaag ttgaaagccg tggtggccgc gacacgggcc gccgccgccg cgagcgggag
     3301 ttcgggggcg tccgtggaca gggcccaggc gccgtagtag gcgtgcacgc gcgccagctc
     3361 gttgcgcgtg tagatgtcgg ccatcttgtg cttgagcgcc tggaagccgc cgatctggcg
     3421 cccgaaggcc ttgcgctcca gcgagtagtc gcgcgagagc tcgaggatgc gcgcggcgcc
     3481 gcccacctgt tcgaaggcca gcagcacggc ggcgccgttc agcacccggc gggccagcgc
     3541 ggcgccgtcg gccggcagct tctgcgccgg cgtgccggcg aacgtgagct gcgccacggg
     3601 gcgggtgggg tcgagcgagg acagcggcct gcggctcacg ccggcgccgg ccaggtcgac
     3661 cagcaccagc tcggtgccgg cgcgcagcac cgcgagaccc gcgatcgcgc cgtccggcac
     3721 cacgcctgcc gtgccgttca gcgtggcgcc gtcgaaggcg aggggcggca gcccgcgata
     3781 actctccccg ctgccgagcg cagcggtggc gaccagttcg ccggcggcga tgcgcggcag
     3841 ccactgctgc ttctgctgct cgctgccgcc gtgcaggatc gcctcggcgg ccaggtagaa
     3901 actcgagctc acgggcactg cggccaggtg ccgaccgatc tcctccgcca ccaggcacag
     3961 ctcgagatag ccgaatcccg caccgccgta ggcttccggg atcgccgcgc cggcggcacc
     4021 catcccgacc aggttgcgcc acacctgcgc gcaacaggcc tccttgccct cgagcaccgt
     4081 gcgcactgtc gccgaagtgc tggtgtcggc cagcatgcgg cgcacctcct gctgcaacag
     4141 gcgctggtcg tcggagaagt cgaaattcac ggcgtttgtc tccttacacg cgtggagaaa
     4201 aagcagggcc ccgcaggacc ctgattggta acggatcgag cgcccggttc agaaccc
//
LOCUS       DAORUQ010000235         4242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26617_length_4242_cov_5.575352, whole genome shotgun sequence.
ACCESSION   DAORUQ010000235 DAORUQ010000000
VERSION     DAORUQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26617_length_4242_cov_5.575352"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLW13_14630"
     CDS             complement(<1..279)
                     /locus_tag="PLW13_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQY71666.1"
                     /translation="MNVSRRIPLGLVGGGPRSLIGGVHRAAAALSERFELKGGAFGTD
                     VSRNRAHARALGLDPERAYAGLDEMLAAESRLAEGERIEVLAVLTPNHL"
     gene            complement(276..1505)
                     /locus_tag="PLW13_14635"
     CDS             complement(276..1505)
                     /locus_tag="PLW13_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71667.1"
                     /translation="MSRMRARLCLMMFLQFFVWGAWFVTLGTFLATNLRASGEQTGLV
                     FATQSWGAIVAPLFVGLVADRFVNAERIFALLHLAGAVLLWRMYAAPEFASFYPLALA
                     YMIVYMPTLALANAVCFRQMRDTTTEFPPVRMWGTVGWIVAGLAISYAFAWDDRANIA
                     AGALRNTFLLAAVASAVLGLYGFSLPATPPVRRGESQVRALLGVEALGLLKDRNFAVF
                     FCSAVLICVPLAFYYQNANLFLAEAGVANPTGTMTIGQISEAGFILLLPWFFHRFGFR
                     RTLLLGMFAWALRYALFAFGNASELLWMLLVGIALHGVCYDFFFVAGQVYTDARAGPR
                     FKASAQGLVTLATYGLGMLIGFWIAGMITDRYTLAAGHDWREVWLFPSGFALVVSGVF
                     LLLFRDTVATVAPEAEQ"
     gene            complement(1523..3214)
                     /locus_tag="PLW13_14640"
     CDS             complement(1523..3214)
                     /locus_tag="PLW13_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQY71668.1"
                     /translation="MSMDFDVIVVGSGVTGGWADTELTEKGLKVLVLDRGRPLTHGSG
                     YTTEHAPPWKIPFGGKPLRELYARDYAVQSKSYAFDETTRHFWVKDSDYPYTQAQDAP
                     FHWLRADVVGGKSLLWARQVYRWSDLDFEANRRDGHGVDWPIRYRDLEPWYAHVERFI
                     GVSGQAEQLAQLPDSVFQPAMQMSVVEKAMKQRIEAQFPARRMTIGRVANLTEPLGDR
                     AACHYCGVCQRGCSPGAYFSSLSSTLPAASRTGNLTLRANSLVEGIDYDAARGRVTGV
                     RVIDTETGARTRFGSRLVFLCGSTIGSVQVLLNSRSESFPQGLANRSGMLGRYVMDHV
                     NGAIAFGITTEFMGRYYYGYRPNGIYIPRFRNLDAQEPDGEFLRGYGFQGGAMALEAP
                     MIATAIPGFGAGFKQALRQPPAWAFFLAGFGECLPYAENRITLDPKLVDRHGMPQVRF
                     DVRFRDNEKKLLQDAAQQARAMLEGAGLQNVLSVATPSTPGDAIHEMGGACMGRDPQR
                     SVLNGWNQSHDVPNLFVTDGSSMSSCGVVNPSLTFMALTARAANHAVEQLRGGRV"
     gene            complement(3296..3898)
                     /locus_tag="PLW13_14645"
     CDS             complement(3296..3898)
                     /locus_tag="PLW13_14645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQY71669.1"
                     /translation="MNRRELLLAAISTMAGVMVGSPTLRVIAGEAPTASASRVVLQGH
                     QPEMVRVLAEMIIPATDTPGAIEAGVPRFIEMMVADWYTDTERGIFLDGLAALDASCR
                     TAYGTDFLGCSAEQRIAALSEAEKSAVAYVSPLPPGFLATMAKLIDERTPFFAKLKEL
                     TVVGYFSSELGATEFLEYNPMPMRYDGDFDFAEVGTHWSH"
     gene            complement(3922..>4242)
                     /locus_tag="PLW13_14650"
     CDS             complement(3922..>4242)
                     /locus_tag="PLW13_14650"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HQY71670.1"
                     /translation="TQWQRTDEWYSFAKSPRAPDIRVLATIDESSYAPGEFFDGTSLA
                     MGADHPMIWKHCVRKGRVFYAALGHTAETFAEPQYRGLLQRAIAWAGRIGSPAASPMP
                     LDCE"
BASE COUNT          754 a         1463 c         1393 g          632 t
ORIGIN      
        1 caggtgattc ggcgtcagta ccgcgagcac ctcgatgcgc tcaccctcgg cgagccggct
       61 ctcagccgcg agcatctcgt cgaggcctgc atatgcgcgc tcggggtcga gaccgagcgc
      121 ccgcgcgtgc gcgcggttgc gcgagacgtc ggtgccgaat gcgcccccct tcagctcgaa
      181 gcgctccgac agcgccgccg cggcgcgatg cacgccgccg atcagcgagc gcgggccacc
      241 gccgaccagg ccgagcggaa tacgacgcga gacgttcatt gctccgcctc gggcgccacg
      301 gtcgccaccg tatcgcggaa cagcaacagg aagaccccgg aaacgacgag tgcgaagccg
      361 gacgggaaca gccacacctc gcgccagtcg tgcccggcgg cgagcgtgta gcggtcggtg
      421 atcatgcccg cgatccagaa gccgatcagc atgccgagcc cgtaggtggc cagcgtgacc
      481 agtccctgcg ccgaggcctt gaagcgcggg cctgcgcgcg cgtcggtgta gacctgcccg
      541 gccacgaaga agaagtcgta gcacacaccg tgcagcgcga tgccgaccag cagcatccac
      601 agcagctcgc tggcgttgcc gaaggcgaac agcgcgtagc gcagggccca cgcgaacatg
      661 cccagcagca gcgtgcgcct gaaaccgaaa cggtggaaaa accacggcag caacaggatg
      721 aagccggcct cggatatctg cccgatcgtc atcgtgcccg tcggattcgc cacgcccgcc
      781 tcggcgagga acaggttcgc attctggtag tagaacgcga gcggcacgca gatcagcacg
      841 gccgagcaga agaaaaccgc gaaattccgg tccttcagca ggcccagggc ctcgaccccg
      901 agcagtgcgc gcacctgcga ctcgccgcga cgcaccggcg gcgtcgccgg cagcgagaag
      961 ccgtagagac cgagcacagc cgaggcaaca gccgcgagca ggaaggtgtt gcgcaacgcg
     1021 cccgcggcga tgttcgcgcg atcgtcccac gcgaacgcgt agctgatcgc gagccctgcg
     1081 acgatccagc ccaccgtgcc ccacatccgc acgggcggga actccgtcgt ggtgtcgcgc
     1141 atctggcgga agcacaccgc gttggcgaga gcgagcgtcg gcatgtagac gatcatgtag
     1201 gcgagcgcca acggatagaa cgaggcgaac tccggcgccg cgtacatgcg ccacagcagc
     1261 accgcgcccg cgaggtgcag cagcgcgaat atgcgctcgg cgttcacgaa gcggtcggcg
     1321 accaggccga cgaagagcgg cgcgacaatg gcaccccagg attgcgtcgc gaagaccagg
     1381 ccggtctgct ctccgctggc gcgcaggttg gtcgcgagga atgtacccag cgtcacgaac
     1441 caggctcccc acacgaagaa ctgcaggaac atcatcaggc acagccgcgc gcgcatcctg
     1501 gacatgaggc cggctccccg cgtcacaccc tgccgccacg caattgttcg accgcatggt
     1561 tcgcagcccg cgcggtgagc gccatgaagg tgagcgaggg attgaccacg ccgcaggaac
     1621 tcatgctgga accatcggtg acgaagagat tgggcacgtc gtgcgactgg ttccagccgt
     1681 tcagcaccga tcgctgcggg tcgcggccca tgcaggcccc gcccatttcg tgaatcgcgt
     1741 ccccgggcgt ggagggcgtc gccaccgaca gcacgttctg caggcccgcg ccctcgagca
     1801 tcgcccgggc ctgctgcgcc gcgtcctgca ggagcttctt ctcgttgtcg cgaaagcgca
     1861 cgtcgaagcg cacctgcggc atgccgtgcc ggtcgacgag cttcggatcc agcgtgatcc
     1921 ggttctccgc gtagggcagg cactcgccaa agccggcgag gaagaacgcc caggcgggcg
     1981 gctgacgcag cgcctgcttg aagcccgcgc cgaagcccgg gatcgcggtg gcgatcatcg
     2041 gcgcctccag cgccatcgcg ccgccctgga agccgtagcc gcgcaggaat tcgccgtcgg
     2101 gctcctgtgc atcgaggttg cgaaagcgcg gtatgtagat cccgttgggg cgatagccgt
     2161 agtaatacct gcccatgaac tcggtggtga tgccgaaggc gatggcgccg ttgacgtgat
     2221 ccatcacata gcgcccgagc atgccgctgc ggttcgcgag accctggggg aagctctcgg
     2281 agcgcgagtt cagcagcacc tgaacgctgc cgatggtgga accgcagaga aagacgagcc
     2341 tggacccgaa gcgcgtacgc gcaccggttt ccgtgtcgat gacgcgcacg cccgtgacac
     2401 gcccgcgtgc cgcgtcgtag tcgattccct cgacgaggct gttcgcgcgc agcgtgagat
     2461 tgccggtgcg gctcgctgcc ggcagcgtcg agctcaggct gctgaagtac gcacccggcg
     2521 aacaaccgcg ctggcacacg ccgcagtagt gacaggcggc gcggtcgccg agcggctcgg
     2581 tgaggttcgc gacccggccg atggtcatgc gccgcgcggg aaactgtgcc tcgatccgct
     2641 gtttcatggc cttctcgacc acggacatct gcatcgccgg ctggaacacg ctgtccggca
     2701 gttgcgccag ctgctcggcc tggccgctca cgccgatgaa acgctcgacg tgtgcgtacc
     2761 acggctcgag gtcgcggtaa cggatcggcc agtcgacgcc gtggccgtcg cgccggtttg
     2821 cctcgaagtc cagatcgctc cagcgataga cctgccgcgc ccagagcagc gacttgccgc
     2881 cgaccacgtc ggcgcgcagc cagtggaaag gtgcatcctg cgcttgcgtg tacgggtagt
     2941 ccgaatcctt gacccagaag tgccgcgtgg tctcgtcgaa agcataggat ttgctctgta
     3001 ccgcgtaatc gcgcgcgtag agctcgcgca gcggcttgcc gccgaagggt atcttccacg
     3061 gcggcgcgtg ttcggtggtg taaccggaac cgtgcgtcag cggcctgccg cgatcgagca
     3121 ccagcacctt cagacccttc tcggtgagct ccgtatcggc ccagcccccc gtgacacccg
     3181 acccgaccac gatgacgtcg aaatccatgc tcattccccg atcctcttgt cgttttcgcg
     3241 cgtcaggccc tttgcacccc tcgacgtcgc gccgcactgc acgccctgcc ggcgctcagt
     3301 gactccagtg cgtccccacc tcggcgaagt cgaagtcacc gtcgtagcgc atgggcatgg
     3361 ggttgtattc gaggaactcc gtggccccga gttcggagct gaaatagccc accactgtca
     3421 gctccttgag cttggcgaag aacggcgtgc gctcatcgat cagtttcgcc atcgtcgcca
     3481 ggaatcccgg cgggagcgga ctcacgtaag cgacggcgct cttctcggcc tcggagagtg
     3541 ccgcaatacg ctgctcggcg ctgcacccga ggaaatccgt gccgtaggcg gtgcggcagg
     3601 acgcatcgag cgccgcgagg ccgtcgagaa agatcccgcg ctcggtatcg gtgtaccagt
     3661 cggcgaccat catctcgatg aaacgcggca caccggcctc gatggcgccc ggcgtatccg
     3721 tcgcggggat gatcatctcg gcgagcacgc gcaccatctc cggctggtgg ccctgcagca
     3781 ccaccctgga ggccgacgcg gtcggcgcct cgcctgcgat cacgcgcagc gtgggcgagc
     3841 cgaccatgac cccggccatc gtggatatgg ccgccagcag cagttcacgt cggttcatcg
     3901 cgattcactc cctgtaggca ttcattcaca atcgagcggc atcggcgagg ccgcgggaga
     3961 gccgatacga cctgcccagg cgatcgcgcg ctgcagcagc ccacggtact gcggctcggc
     4021 gaatgtctcc gcggtatgcc ccagcgcggc atagaacacc cggcccttgc gcacgcagtg
     4081 cttccagatc atcgggtggt ccgcgcccat cgcgagcgac gtgccgtcga agaattcgcc
     4141 cggtgcatag ctgctctcgt cgatcgtcgc cagcacgcgg atatccggcg cgcgcgggct
     4201 cttcgcaaag gagtaccact cgtcggtgcg ctgccattgc gt
//
LOCUS       DAORUQ010000236         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26710_length_4234_cov_5.965542, whole genome shotgun sequence.
ACCESSION   DAORUQ010000236 DAORUQ010000000
VERSION     DAORUQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26710_length_4234_cov_5.965542"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLW13_14655"
     CDS             complement(<1..321)
                     /locus_tag="PLW13_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQY71671.1"
                     /translation="MLGRRRVPDYSGDPADIRRAAIDLLSRREHCAAEIRAKLLARFG
                     KEAPVEPELERLLAEGLLSDARFAETFMRAHRNKGHGPLRILRDLAQRGVASELAEAA
                     VDSRS"
     gene            complement(342..1370)
                     /gene="recA"
                     /locus_tag="PLW13_14660"
     CDS             complement(342..1370)
                     /gene="recA"
                     /locus_tag="PLW13_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQY71672.1"
                     /translation="MDANKQKALDAALAQIERQFGKGTVMRMGDRTQDAIPAISTGSL
                     GLDIALGIGGLPKGRVVEIYGPESSGKTTLTLSVIAECQKLGGTAAFIDAEHALDPGY
                     AEKLGVKVEDLIVSQPDTGEQALEVVDMLVRSGAVDVVVVDSVAALTPRAEIEGEMGD
                     SHVGLHARLMSQALRKITGNINRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFYA
                     SVRLDIRRTGAVKEGDEVIGSETRVKVVKNKVAPPFRQAEFDILYGRGIYRMGEVIDL
                     GVKLGLVEKSGAWYSCKGERIGQGKANAARFLAENPAIAGPLEAEIRAKALATGAADV
                     IVPELEEA"
     gene            1664..3382
                     /locus_tag="PLW13_14665"
     CDS             1664..3382
                     /locus_tag="PLW13_14665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HQY71673.1"
                     /translation="MSRSITVLLAALCLGATAHAADTVEPPSAIDTLKQKTGKLLMNV
                     KPFMVEGAYSQFHPLLQSVRENQHLEPVIPRAQQQAQAQGKLAEWMKQHDGKRPNILI
                     LLVDDMGWGDPGAYGGGETLGSPTPNIDALARDGLTLTSAYAQPSCTPTRAAIYTGRL
                     PVRSGLIRPTMQGEVAENAANEVLAAKLLGQAGYRTGLSGKWHLGEQAGSFPTDMGFE
                     RFFGMLGVVDMYQDWQMAANSPEIVTKPERFAAYKAFHERGDWLMQGVAGQPLERVKP
                     MTLDTTANMDQDFAAWSENFIREAAQDGKPFYLVHAFSKVHFFNIPAEGYAGRSPAGT
                     PYRDGVVEVDDVVGRLLKTLKDTGQLDNTFVFFTSDNGPEEDSWPDSGHTPFRGAKGT
                     TWEGGVRVPGIAYWPGMIAPGRRSDGLFDLMDLFNTSLALAGATERIPDDRYIDGIDQ
                     SSFLLADDGQSQREIVWMWNQNDFAAMRVQEFKAHYRLYEIDNDNPDALGGLNNSRTD
                     TPMNPWFYNLYNDPKERVPKLVEKVWAAGMMNFPLYQHAMSFRVYPNQPALATLQSTL
                     DMMKSQ"
     gene            3379..4113
                     /locus_tag="PLW13_14670"
     CDS             3379..4113
                     /locus_tag="PLW13_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY71674.1"
                     /translation="MSERVPRAAPARRPAVAGDGAGRGGTGKSNADATRRALSPEDFI
                     AATLELVDAHGVDAVSMRRVAEHLGVSPMAAYRHFRDKEELLVRALDAFAGRAELLPL
                     DSLAWDDWVRAVARTMHEALAAHPGWIPLLGSLRLGQHAAAATRAFVERLVAEGFTPE
                     RALRAWFAVNQVVIGAVCMGGPLLPPPADAPAPSAAGPRGAGPVAHEMAAELRAIGPV
                     AQLDIGLEFILEALEAERTPDGRRFP"
BASE COUNT          697 a         1485 c         1402 g          650 t
ORIGIN      
        1 cgaacgcgag tccactgcgg cctcggccag ctcgctggcg acgcctcgct gcgcaagatc
       61 gcgcaggatc cgcaagggtc catggccctt gttgcggtgc gcgcgcatga aggtctcggc
      121 gaagcgcgcg tcgctcagca ggccctcggc gagtagccgc tcgagctcgg gctcgaccgg
      181 cgcttccttg ccgaagcgcg cgaggagctt ggcgcggatc tccgccgcgc agtgctcgcg
      241 ccgcgacagc aggtcgatcg cggcgcggcg gatgtcggcc gggtcaccgg aatagtccgg
      301 cacccggcga cgccccagca cgggagcagg ctcccgtgcg ctcaggcttc ttccagctcc
      361 ggcacgatca cgtcggccgc gccggtagcc agcgccttgg cgcggatctc ggcttccagc
      421 ggaccggcga tcgcggggtt ctcggcgagg aagcgtgccg cgttggcctt gccctggccg
      481 atgcgctcgc ccttgcagga gtaccatgcg ccggatttct cgaccagccc gagcttcacg
      541 cccagatcga tcacctcgcc catgcggtag atgccgcgac cgtagaggat gtcgaactcg
      601 gcctggcgga acggcggcgc gaccttgttc ttcacgacct tgacgcgcgt ctcgctgccg
      661 atcacctcgt cgccctcctt caccgcgccg gtacgccgga tgtcgagacg caccgaggcg
      721 tagaacttga gcgcgttgcc gccggtggtg gtctcggggg agccgaacat cacgccgatc
      781 ttcatgcgga tctggttgat gaagatcacc agcgtgttgg agcggttgat gttgccggtg
      841 atcttgcgca acgcctggct catcaggcgc gcatgcaggc cgacgtgcga atcgcccatc
      901 tcgccctcga tctcggcgcg cggcgtcagc gcggccaccg agtcgaccac caccacgtcg
      961 accgcgcccg agcgcaccag catgtcgacc acctcgagcg cctgctcgcc ggtgtcgggc
     1021 tgggacacga tcaggtcctc gaccttcacg ccgagcttct cggcataacc cgggtcgagc
     1081 gcgtgctcgg cgtcgatgaa ggcggccgtg ccgcccagct tctggcactc ggcgatcacc
     1141 gagagcgtca acgtcgtctt gccggaggat tccggcccgt agatttccac cacccggccc
     1201 ttcggcaggc cgccgatacc gagcgcgatg tccagtccga gcgaccccgt cgagatggcc
     1261 ggtatggcat cctgcgtccg gtcccccatg cgcatcacgg tacccttgcc aaactggcgt
     1321 tcgatctgag ccagcgccgc atccagtgct ttctgcttgt tcgcgtccat ttgtagcttc
     1381 cttcaggtgc tgtgaatgag gattgcccaa ggcctgtaca tttaagcagc atgctggatg
     1441 aatatccagc ccttggcgtc atccgttgcc gtgcttccct gccgtggcgg cgcgtgcgga
     1501 caacgaagcg cactataact cgcccgcggg ccaccgcgca tcgcgacaag cactgccagg
     1561 cacggcccga ttgtcatgca gcgctgcaca tgtttacact gtacacatgc aacaccgcat
     1621 ccaccggccc ggtgggttgc catcgcgtgc ggaggcacaa tccatgtccc gatcgatcac
     1681 cgtcctgctg gcggcactct gccttggcgc cacggcccac gcggccgaca cggtcgagcc
     1741 accctcggcg atcgacaccc tgaagcagaa gaccggcaag ctgctgatga acgtgaagcc
     1801 cttcatggtc gagggcgcct acagccagtt tcaccccctg ctgcagtcgg tgcgcgagaa
     1861 ccagcacctc gagcccgtga taccgcgcgc gcagcaacag gctcaggcac agggcaagct
     1921 cgccgagtgg atgaagcagc atgacggcaa gcgcccgaac atcctgatcc tgctggtcga
     1981 cgacatgggc tggggcgatc ccggcgccta cggcggcggc gagacgctgg gctcgcccac
     2041 gccgaacatc gacgcgctcg cccgtgacgg actcacgctg acctcggcct acgcgcagcc
     2101 gagctgcacg cccacgcgcg ccgcgatcta caccgggcgc ctgccggtac gcagcggcct
     2161 gatccgcccc accatgcagg gcgaggtggc cgagaacgcg gccaacgagg tgctggcggc
     2221 gaagctgctc gggcaggccg gctaccgcac cggcctgagc ggcaagtggc acctcggcga
     2281 gcaggccggc tccttcccca ccgacatggg cttcgagcgc ttcttcggca tgctcggcgt
     2341 ggtcgacatg taccaggact ggcagatggc cgccaacagc ccggagatcg tcaccaagcc
     2401 cgagcgcttc gccgcctaca aggccttcca cgagcgcggt gactggctga tgcagggcgt
     2461 ggccggccag ccgctcgagc gcgtgaagcc gatgacgctc gacaccaccg cgaacatgga
     2521 ccaggacttc gccgcgtggt ccgagaactt cattcgcgag gcagcgcagg acggcaaacc
     2581 gttctacctg gtgcacgcct tcagcaaggt gcatttcttc aacatcccgg ccgagggcta
     2641 cgcgggacgc tcgccggccg gcacgcccta ccgcgatggc gtggtcgagg tcgacgacgt
     2701 cgtgggccgc ctgctgaaga cgctgaagga caccggccag ctggacaaca ccttcgtgtt
     2761 cttcacctca gacaacggcc ccgaggagga cagctggccc gacagcggcc acacgccctt
     2821 ccgcggcgcc aagggcacca cctgggaggg cggcgtgcgc gtgccgggta tcgcgtactg
     2881 gccaggcatg atcgcaccgg gccgacgctc ggacggactc ttcgacctga tggacctgtt
     2941 caacacctcg ctcgcgctcg ccggtgccac cgagcgcatc ccggacgacc gctacatcga
     3001 cggcatcgac cagagttcct tcctgctcgc cgacgacggg cagagtcaac gcgagatcgt
     3061 gtggatgtgg aaccagaacg acttcgccgc gatgcgcgtg caggaattca aggcgcacta
     3121 ccggctctac gagatcgaca acgacaaccc ggatgcgctc ggcgggctga acaactcgcg
     3181 caccgacacg ccgatgaacc cgtggttcta caacctgtac aacgacccca aggagcgcgt
     3241 gcccaagctg gtggagaagg tctgggccgc cgggatgatg aacttcccgc tgtaccagca
     3301 cgcgatgagc ttccgcgtgt acccgaacca gccggcgctg gcgacgctgc aatcgactct
     3361 ggacatgatg aagagccaat gagcgaacgc gtcccgcgag cagcgcctgc ccgccggccc
     3421 gccgtggcag gcgatggcgc ggggcgcggc ggcaccggga agagcaatgc cgacgcgacg
     3481 cgccgtgcgc tgtcgccgga ggatttcatc gccgccacgc tggaactggt cgacgcgcac
     3541 ggcgtcgacg ccgtctcgat gcggcgcgtc gcggaacacc tgggcgtgag tccgatggcg
     3601 gcctaccggc atttccgcga caaggaagaa ctgctggtgc gcgcgctcga tgcgttcgcc
     3661 ggccgcgcgg aactgctccc gctggactcg cttgcgtggg acgactgggt ccgcgcggtg
     3721 gcgcgcacga tgcacgaggc actggccgcg cacccgggat ggatcccgct gctgggctcg
     3781 ctgcgactgg gccagcacgc tgccgccgcg acgcgcgcct tcgtcgaacg cctggtcgcc
     3841 gagggcttca cgcccgagcg cgcgttgcgc gcctggttcg ccgtgaacca ggtcgtgatc
     3901 ggcgcggtgt gcatgggcgg gccgctgctc ccgcctccgg cagatgcgcc ggcgccgagc
     3961 gcggccggtc cgcgcggcgc cggacccgtg gcgcacgaga tggccgcgga actgcgcgcg
     4021 atcggcccgg tagcgcagct ggatatcggg ctggagttca tcctcgaggc gctggaggcg
     4081 gagagaaccc cggacggaag gcgatttccc tgaggcacag atcgctcgag gagagcagct
     4141 cggtcacctc cgtcgtgggt acgcggcgcc attcccttga aaatacgggt tttgacagca
     4201 acgccgatgc gggtcagcgc accaccgtgg cgtc
//
LOCUS       DAORUQ010000237         4217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26882_length_4217_cov_5.927919, whole genome shotgun sequence.
ACCESSION   DAORUQ010000237 DAORUQ010000000
VERSION     DAORUQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26882_length_4217_cov_5.927919"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1461
                     /locus_tag="PLW13_14675"
     CDS             <1..>1461
                     /locus_tag="PLW13_14675"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71675.1"
                     /translation="VSSIAAAGLYKGTFTEDMFEEATGLDNPYYRTKHDAEGLVRREK
                     GIPWRIYRPGVVVGHSASGEMDKIDGPYYFFPAIRQVSNALPAWLRAIMVQGGTINIV
                     PVDFVVDAMDYLMHARGQDGQTFFLTDPKGITTGDLVRVLLQAARGPRLSVVSGGGLE
                     KLLGQVPVGRLPGVVPLGRALGAVMEKYGIPEESLKFVGYPTRFDSTKTRKLLARGGI
                     ECPPFESYADVIWEYWEEHLAPEARPRRGRSPSWVRRLERALGRSDPRRLARAVKGKV
                     VVVTGASSGIGRDCALKLAAAGAIVVLAARTPEKLDETIRDIHELGGEGYACPCDIAD
                     MTDCDRFVASVLEQHGRVDILINNAGRSIRRSVQHSFDRFHDYERTMQLNYFGALRLI
                     MGFAPGMLERESGHIVNISSIGVLASPPRFSAYVASKAALDAFSWCAASEFADRNVRF
                     TTINMPLVRTPMIAPTRLYDAFPTLSPEQAANLVMKA"
     assembly_gap    1462..1561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1663..2217)
                     /locus_tag="PLW13_14680"
     CDS             complement(1663..2217)
                     /locus_tag="PLW13_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71676.1"
                     /translation="MRAATILLAVAVILGGCVAAGQTKEDKQRHIENMRKEVLTDLYK
                     INPSVQQQIAQAPGYAVFSNWNAYLIFPSVGQGYGVVVDNVSGAHTYMKMGSLGGGLG
                     VGVKDFRAVYVFEKRHIMERFIEQGLNVGAQADAAAKSGGQGDAVGGELLLDGIRVYQ
                     LTESGLALQATIQGTKYWKDPELN"
     gene            2392..3456
                     /gene="fba"
                     /locus_tag="PLW13_14685"
     CDS             2392..3456
                     /gene="fba"
                     /locus_tag="PLW13_14685"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HQY71677.1"
                     /translation="MALISMRQLLDHAAEHDYGVPAFNVNNLEQMRAIMEAADESCSP
                     VIVQASAGARKYAGANFLRHLILAAIEEFPHIPVVMHQDHGVSPAVCQRSIQLGFSSV
                     MMDGSLGEDGKTPTDYDYNVRVTRETVAMAHACGVSVEGEIGCLGSLETGTAGEEDGI
                     GAEGILSHDQMLTDPEEAARFVKDTGVDALAIAIGTSHGAYKFSRKPTGDILAIGRIK
                     EIHARIPNTHLVMHGSSSVPQDWLAIINEFGGEIPETYGVPLEEIQEGIRNGVRKVNI
                     DTDLRLASTGAIRRFMAKNPSEFDPRKYLKETVTAMKAICRERYEAFGTAGQADRIRP
                     LSLEAMYVRYARGELNQKVS"
     gene            3463..>4217
                     /locus_tag="PLW13_14690"
     CDS             3463..>4217
                     /locus_tag="PLW13_14690"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY71678.1"
                     /translation="MTGSPFARSRIAARSFDPEAVRAAMRPLEFSGLSVVSGAEQDYF
                     AFYGIDLAHRVPGVTHHFGRLAANGFEIACHFYELTAARGTCVLLHGYFDHAGLYGSL
                     IEHCLRQGHSVLIWDLPGHGLSNGPQASIHDFEDYTDVLALVLEHHRERLPGPLYAIG
                     QSTGGAVLMSWAFRKCRSAAECPFARIALLAPLVRPARWRHVNLLYTLLRPFRSGVRR
                     KFMPNSGDTEFLAFVRNDPLQSTILPVQWVGAMR"
BASE COUNT          690 a         1404 c         1346 g          677 t
ORIGIN      
        1 cgtgagctcg atcgcggcgg ccgggctcta caagggcacc ttcaccgagg acatgttcga
       61 ggaggcgacc ggcctcgaca atccctacta ccgcaccaag cacgatgccg aagggctggt
      121 gcggcgcgag aagggcatcc cgtggcgcat ctaccgcccc ggcgtcgtcg tcggccattc
      181 cgccagcggc gaaatggaca agatcgacgg accctattac ttcttcccgg cgatccgcca
      241 ggtcagcaac gccctgcccg cctggctgcg cgcgatcatg gtgcagggtg gcaccatcaa
      301 catcgtgccg gtggatttcg tggtcgacgc catggactac ctgatgcacg cgcgcggcca
      361 ggacgggcag accttcttcc tgaccgatcc gaagggcata accaccggcg acctggtccg
      421 cgtgctgctg caggccgcgc gcgggcccag gctcagcgtc gtgagcggcg gtggcctgga
      481 gaagctgctc gggcaggtgc cggtgggacg actgcccggc gtggtgccgc tgggcagggc
      541 gctgggcgcg gtgatggaaa agtacggcat ccccgaggaa tcgctgaagt tcgtcggcta
      601 tccgacgcgc ttcgactcga ccaagacacg caagctcctc gcccgcggcg gcatcgagtg
      661 cccgcccttc gaatcctacg ccgacgtgat ctgggagtac tgggaagagc acctggcgcc
      721 cgaggcgagg ccgcgccgcg gccgttcgcc gtcctgggtg cgccggctgg agcgggcgct
      781 gggacgcagt gatccgcgcc ggctggcgcg cgcggtcaag ggcaaggtcg tggtggtcac
      841 gggtgcgagc tccggcatcg ggcgcgactg cgcgctgaag ctggccgcgg ccggggccat
      901 cgtggtgctg gcggcgcgca ccccggaaaa gctggacgaa accattcgcg acatccacga
      961 gctgggcggc gagggctacg cctgtccctg cgacatcgcc gacatgaccg actgcgaccg
     1021 cttcgtggcc agcgtgctgg agcagcacgg ccgggtcgac atcctgatca acaacgcggg
     1081 gcgctcgatc cgccgctcgg tgcagcattc cttcgaccgc ttccacgact acgagcgcac
     1141 gatgcagctc aactacttcg gtgcactgcg cctgatcatg ggtttcgcgc ccggcatgct
     1201 cgagcgcgag agcggccata tcgtcaacat ttcctcgatc ggcgtgctcg cgagcccgcc
     1261 gcgcttctcg gcctacgtgg catcgaaggc cgcgctggat gcgttctctt ggtgcgcggc
     1321 ctcggaattc gccgaccgca acgtgcgctt caccaccatc aacatgccgc tggtgcgcac
     1381 gccgatgatc gcgccgaccc ggctctacga cgccttcccc accctctcgc ccgagcaggc
     1441 cgcgaacctg gtcatgaagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 ntgtaggtcg ggcttcagcc cgacaagggt tctaacccgc cacgcctagg gcgtacccgt
     1621 cggcatgaat gccgacccac atgaatgccg acccgcgtgc ggtcaattga gctcgggatc
     1681 tttccagtac ttggtgccct ggatggtggc ctgcagcgcc aggccggatt cggtcaactg
     1741 gtagacgcgg atgccgtcga gcagcagttc gccgcccacc gcatcaccct gcccgcccga
     1801 cttggctgcc gcatcggcct gggcgcccac gttgaggccc tgctcgatga agcgctccat
     1861 gatgtggcgt ttctcgaaca cgtagactgc gcggaagtcc ttcacgccga cgccgaggcc
     1921 gccgccgagc gagcccattt tcatgtaggt gtgcgcgccg ctcacgttgt cgaccaccac
     1981 gccgtagccc tgccccaccg aggggaagat caggtaggca ttccagttcg agaacaccgc
     2041 gtatccgggt gcttgggcga tctgctgctg cacggaagga ttgatcttgt agagatccgt
     2101 gagcacttcc ttgcgcatgt tctcgatgtg gcgttgcttg tcctccttcg tctgcccggc
     2161 ggcgacgcag ccgcccagga tgaccgcaac agcaaggagg atcgtcgctg ctctcatgga
     2221 atgctcctga agtatgtaaa accggcgcca ttgtagaccg attgcggcga tcgaggaatg
     2281 tgtggcaatg acaggcgcgg ggccgctgca ttagactcgc ctcccatttt gtcgatcgaa
     2341 gcaccggccg acgcgccatt gcgcacggcc ggcaacagcc ggaggtatcc catggcattg
     2401 atcagcatgc gccagttgct ggaccacgcc gccgagcacg actacggcgt gccggcgttc
     2461 aacgtgaaca acctcgaaca gatgcgcgcc atcatggagg ccgccgacga gagctgcagc
     2521 ccggtgatcg tgcaggcctc ggccggcgcg cgcaaatacg ccggtgccaa cttcctgcgc
     2581 cacctgatcc ttgcggcgat cgaggaattc ccgcacatcc cggtcgtgat gcaccaggac
     2641 cacggcgtct cgccagccgt gtgccagcgt tcgatccagc tcggcttcag ctcggtgatg
     2701 atggacggct cgctcggcga ggacggcaag accccgaccg attacgacta caacgtgcgc
     2761 gtgacgcgtg agacggtcgc gatggcgcat gcctgcggcg tgtcggtgga gggcgagatc
     2821 ggctgcctcg gctcgctgga gaccggcacc gcgggcgagg aagacggcat cggcgccgaa
     2881 ggcatactga gccacgacca gatgctcacc gatcccgagg aggccgcgcg cttcgtgaag
     2941 gacaccggcg tggacgcgct ggcgatcgcc atcggcacca gccacggcgc ctacaagttc
     3001 tcgcgcaagc ccaccggcga catcctcgcg atcggtcgca tcaaggaaat ccacgcgcgc
     3061 atccccaaca cgcacctggt gatgcacggc tcctcctcgg tgccgcagga ctggctcgcg
     3121 atcatcaacg agttcggcgg cgagattccc gagacctacg gcgtgccgct ggaggaaatc
     3181 caggagggca tccgcaacgg cgtgcgcaag gtgaacatcg acaccgatct gcgactcgcc
     3241 tcgaccggcg cgatccgccg tttcatggcg aagaacccct cggagttcga cccgcgcaag
     3301 tacctgaagg aaacggtcac ggcgatgaag gcgatctgca gggaacgcta cgaggccttc
     3361 ggcaccgcgg gccaggccga caggatcagg ccgctgtcgc tggaagcgat gtatgtccgc
     3421 tacgcccgcg gcgaactgaa ccagaaggtc agctgagagc ccgtgaccgg cagtcccttc
     3481 gcccgaagtc gcattgccgc gcgcagcttc gaccccgagg ccgtgcgcgc cgcgatgcgg
     3541 ccgctggagt tctccgggct gagcgtcgtt tccggcgccg agcaggacta tttcgccttc
     3601 tacggtatcg acctcgcgca tcgcgtgccg ggtgtcaccc atcacttcgg gcgactcgcg
     3661 gcgaacggtt tcgagatcgc ctgccatttc tacgagctca ccgccgcacg tggcacctgc
     3721 gtcctgctgc atggctactt cgatcacgcg gggctctatg gcagcctgat cgagcactgt
     3781 ctgcgtcaag gccacagcgt gctgatctgg gacctgcccg gccacgggct gtcgaacggt
     3841 ccgcaggcca gcatccacga cttcgaggac tacacggacg tgctcgcact ggtgctcgag
     3901 caccatcgcg agcgcctgcc gggaccgctg tacgccatcg gccagagcac cggcggtgcg
     3961 gtgctgatga gctgggcctt ccgcaagtgc cgttccgcgg ccgagtgccc gttcgcgcgc
     4021 atcgcgctgc tggcaccgct ggtgcggccc gcgcgctggc gccacgtgaa cctgctgtac
     4081 accctgctgc gcccgttccg ctccggcgtg cggcgcaagt tcatgccgaa ctccggcgac
     4141 accgaattcc tcgccttcgt gcgcaacgat ccgctgcaga gcacgatcct gccggtgcag
     4201 tgggtcggcg cgatgcg
//
LOCUS       DAORUQ010000238         4209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_26964_length_4209_cov_5.274916, whole genome shotgun sequence.
ACCESSION   DAORUQ010000238 DAORUQ010000000
VERSION     DAORUQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26964_length_4209_cov_5.274916"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLW13_14695"
     CDS             <1..597
                     /locus_tag="PLW13_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71679.1"
                     /translation="TQPAELGRQAAKSKATQDKIINAVIALIREGGFAAASSTRIARK
                     AGVSWGAVQHHFGGKHEILEQVLARSHEKFDASMSGEEFTRGALEKRVERFINAAWEH
                     YRGDEYMATLEILLASRAEGGDGASIKLNHHSHLALWRRIFHDCPLTDRAMQEAIYTV
                     HCMLTGYVIEVVLEPEFFDVKRYLQRLRRILLAMLTES"
     gene            635..2158
                     /locus_tag="PLW13_14700"
     CDS             635..2158
                     /locus_tag="PLW13_14700"
                     /inference="COORDINATES: protein motif:HMM:NF005510.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY71680.1"
                     /translation="MSETDRSRRRVLAGLGLAGLAASVGCEPRQPAASAEVRAWDHSA
                     DVVIAGSGAAAISAAIEARAAGAEVLVLERLPKLGGSSAMSGGVCYLGGGTPLQKALG
                     FEDSAEAMHDFIVAAGGLHPRLDKIALYCEASLEHFQWMVDHGVRYIEKFSQEKELPY
                     DDSSLYYSGCELAHPWRDIARPAPRGHVPAVMGHTGGRAMMEALLAAAQAAGVKMMTG
                     VSCERLVRESDGRVTGIQFTENGATRSARARRGVVLACGGFIHNREMVRRYAPELYEC
                     STPWANAGDLGIGIQMGMGVGAAAVRMHQGFAILPVYPPERVIKGIVVNARAQRFIAE
                     DSYYACIGHETAYTQHGKAFLVTDADCSYDPGDFRMPLLAEAASIGELESKGGFVPGT
                     LAHTVATYNAAAARGEDPLLHKHPKFLAPLAKAPLRLYELDVTRAFCCSHTFGGLETT
                     VDSQVLDAFGGVIPGLYAAGRTTAGLPTAPYIASGISVGDCTFFGRRAGRHAARETA"
     gene            2158..2541
                     /locus_tag="PLW13_14705"
     CDS             2158..2541
                     /locus_tag="PLW13_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY71681.1"
                     /translation="MNGVRLAGVALALLSALALADMPAGDAERGRVVFGSCRTCHWPE
                     QGAGHQNGPALWNIFGRKAGTQEGFGYYSQALKDSGLVWTPEYLDAWLANPATFIPGT
                     TMMTPGVPDAQRRADLIAYLQRFRE"
     gene            complement(2597..3049)
                     /locus_tag="PLW13_14710"
     CDS             complement(2597..3049)
                     /locus_tag="PLW13_14710"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HQY71682.1"
                     /translation="MSQQAANKDLVTRFWNDVYVARDYDAVGRYFATDGVYADVPVPD
                     SAATGPQAVAMRLRIGHEPVERFTHQIHRMVSEGSAVITEHTETWHFHTGEVVALPFV
                     SVMEVEDGMIRLWRDYWDVNTLMSKAPPWWLEHIMKFSVADFAHKNPA"
     gene            3239..>4209
                     /locus_tag="PLW13_14715"
     CDS             3239..>4209
                     /locus_tag="PLW13_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HQY71683.1"
                     /translation="MKLGLQLGYWQKHPTPRFIELAQVAESLGFDSVWTAEAYGSDCF
                     TPLAAIAASTKRIRLGTGVMQISARTPTCAAMTALTLDHISNGRLILGVGVSGPQVVE
                     GWYGQPFTKPLERTREWIQIFRQAIAREQPLEFHGEQYNLPCKNGMGLGKPLKSITHP
                     LRKRIPVVLGAEGPKNVALAAEAFDGWLPIFCSPYKMDIFADSLQAAGPDFEIAAMVN
                     LAINDDLQQALVPGKWTMALYLGGMGAKGMNFHKNLLGRMGYADDAQKIQDLFLSGRQ
                     QEAVDAVPDALVDEISLLGPHGRIRERLQDWKKTRVSSLLIGHSGDF"
BASE COUNT          680 a         1392 c         1480 g          657 t
ORIGIN      
        1 acgcaacccg cagagctcgg acgtcaggcg gcgaagagca aggcgacaca ggacaagatc
       61 atcaatgccg tcatcgcgct gatccgcgaa ggcggttttg ccgcggcgag ttccacgcgc
      121 atcgcgcgca aggcgggagt gtcctggggc gcggtgcagc accacttcgg cggcaagcac
      181 gagatcctcg agcaggtgct ggcgcgctcg cacgagaaat tcgatgcgtc gatgtctggc
      241 gaggagttca cgcgcggcgc gctggaaaag cgcgtggagc ggttcatcaa cgcggcgtgg
      301 gagcattacc gcggcgatga gtacatggcg acgctggaaa tcctgctggc cagccgtgcc
      361 gagggcggcg atggcgccag catcaagctg aaccaccact cgcacctcgc gttgtggcgg
      421 cgcatcttcc atgactgccc gctcacggat cgcgccatgc aggaggcgat ctacaccgtg
      481 cactgcatgc tcacgggcta cgtcatcgag gtggtgctgg agcccgaatt cttcgatgtg
      541 aagcgctacc tgcagcgcct gcgccgcatc ctgctggcga tgctcaccga gagctgagtg
      601 acacgcgcga tcaggagacc ggcggagtga atacatgagc gagaccgata gatcacgccg
      661 tcgcgtgctg gccggcctgg ggctcgcggg acttgcggcc agcgtgggct gcgaaccgcg
      721 ccagcccgcc gccagcgccg aggtgcgcgc ctgggaccac agcgccgacg tggtgatcgc
      781 cggctccggg gccgctgcca tcagcgccgc catcgaggcg cgcgcggcgg gcgccgaggt
      841 gctggtgctc gagcgcctgc cgaagctcgg tggctcgtcg gcgatgtcgg gcggggtgtg
      901 ctacctcggc ggcggcacgc cgctgcagaa ggcgctcggc ttcgaggaca gcgcggaggc
      961 gatgcacgat ttcatcgtcg ccgcgggcgg cctgcatccg cggctcgaca agatcgcgct
     1021 ctactgcgag gcaagcctcg agcacttcca gtggatggtc gaccacggcg ttcgctacat
     1081 cgagaagttc agccaggaga aggagctgcc ctacgacgac tcctcgctgt attactccgg
     1141 ctgcgagctg gcgcacccgt ggcgcgacat cgcgcgtccg gctccgcgcg ggcacgtgcc
     1201 ggcggtgatg gggcacaccg gcgggcgcgc gatgatggaa gcgctgctcg cggcggcgca
     1261 ggctgccggc gtgaaaatga tgaccggcgt gtcctgcgag cggctggtgc gcgagagcga
     1321 cggacgtgtg acaggcatcc agttcaccga aaacggggcc acgagatcgg cgcgggcgcg
     1381 ccgcggcgtg gtccttgcct gcggcggttt catccacaac cgcgagatgg tgcgccgcta
     1441 cgcgccggag ctctacgagt gctccacgcc gtgggcgaac gcgggcgatc tcggcatcgg
     1501 catccagatg ggcatgggcg tgggcgcggc ggcggtgcgc atgcaccagg gctttgcgat
     1561 cctgccggtc tacccgcccg agcgcgtgat caagggcatc gtggtcaacg cgcgcgcgca
     1621 gcgcttcatt gccgaggatt cgtactacgc gtgcatcggc cacgagaccg cctacacaca
     1681 gcacggcaag gccttcctcg tgaccgacgc cgactgcagc tacgatccgg gcgatttccg
     1741 catgccgctg ctcgccgagg ccgcgagcat cggggagctc gagtccaagg gcggtttcgt
     1801 ccccggcacg ctggcgcaca cggtcgcgac gtacaacgcg gccgcggcgc gcggcgagga
     1861 tccgctgctg cacaagcacc cgaagttcct cgcgccgctg gcgaaggcgc cgttgcgtct
     1921 ctacgagctc gacgtcacgc gcgcgttctg ctgttcgcac accttcggcg ggctggagac
     1981 cacggtggat tcgcaggtgc tcgatgcctt cggcggcgtg atccccggct tgtatgccgc
     2041 cgggcgcacc accgccggcc tgcccaccgc gccgtatatc gccagcggca tatcggttgg
     2101 cgactgcacg ttcttcggac ggcgcgcggg ccgccatgcg gccagggaga cggcgtgatg
     2161 aacggggtgc gtctcgcggg cgtcgcgctg gcgctgctga gcgccctggc gctggcggat
     2221 atgcccgcgg gcgacgccga acgcgggcgc gtggtgttcg gttcctgccg cacctgtcac
     2281 tggcccgaac agggcgccgg ccaccagaac ggccctgcgc tgtggaacat tttcggtcgc
     2341 aaggccggca cgcaggaagg cttcggctac tactcgcagg cgctgaagga ttccggcctc
     2401 gtctggacgc cggagtatct cgacgcctgg ctcgcgaatc cggcgacctt catcccgggc
     2461 accacgatga tgacgcccgg ggtgcccgat gcgcagcgcc gcgccgatct gatcgcctac
     2521 ctgcagcgct ttcgcgagta gcgccggctc ccgtgggttc ggcttcaacc ggacaattgt
     2581 ccggctgaat gcggacctat gcggggtttt tgtgagcgaa atccgcgacg ctgaacttca
     2641 tgatgtgctc gagccaccac ggcggcgcct tcgacatcag cgtgttcacg tcccagtagt
     2701 cgcgccacag ccggatcatg ccgtcctcga cttccatcac cgagacgaag ggcagggcga
     2761 ccacctcgcc ggtgtggaaa tgccaggtct cggtgtgctc ggtgatcacg gcactgccct
     2821 cggagaccat gcggtggatc tggtgcgtga agcgctccac cggctcgtgc ccgatgcgca
     2881 ggcgcatcgc cacggcctgt ggcccggtcg ccgcggaatc cggcaccggg acgtcggcgt
     2941 agacgccgtc ggtggcgaaa tagcgcccga ccgcgtcgta gtcgcgcgcg acgtagacat
     3001 cgttccagaa acgcgtgacg agatccttgt tggcggcctg ctgtgacatg cggtgtctgc
     3061 ccggggactg ttcgtgcggc gagagtaggg tggggcggcc gcacggcgca agttaccgaa
     3121 cggcaagccg cgctgagcgc tttggtcagc cgtcgcgcgg cgcagcggtc actgatccgc
     3181 gccccgcgcc gccctatact cgctgcgcca ctgaagccac cccgcacgga gaaccctcat
     3241 gaaactcgga ctgcaactcg gatactggca aaagcacccc acgccgcggt tcatcgaact
     3301 ggcgcaggtg gccgagagcc tgggcttcga ttccgtgtgg accgcggagg cctacgggtc
     3361 ggactgcttc acgccgctgg cggcgatcgc ggcctcgacg aagcgcatcc ggctcggcac
     3421 cggcgtgatg cagatttccg cgcgcacgcc gacctgtgcc gcgatgaccg cgctcacgtt
     3481 ggaccacatc tccaacgggc ggctgatcct cggtgtcggg gtgtcgggac cgcaggtcgt
     3541 ggagggctgg tacgggcagc cgttcacgaa gccgctggag cgcacgcgcg agtggatcca
     3601 gatcttccgc caggcgatcg cgcgcgagca gccgctcgag ttccacggcg agcagtacaa
     3661 cctgccgtgc aagaacggca tggggctcgg caagccgctg aagagcatta cccatccgct
     3721 gcgcaagcgc attcccgtgg tgctcggcgc cgagggcccg aagaacgtcg cgctcgcggc
     3781 cgaggccttc gacgggtggc tgccgatctt ctgctcgccg tacaagatgg acatcttcgc
     3841 cgacagcctg caggcggcag gtccggattt cgagatcgcc gcgatggtga acctcgcgat
     3901 caacgacgac ctgcagcagg cactggtgcc gggcaagtgg accatggcgc tgtatctcgg
     3961 cggcatgggc gccaagggca tgaacttcca caagaacctg ctggggcgca tgggctacgc
     4021 cgacgacgcg cagaagatcc aggacctgtt cctctcggga cgccagcagg aagcggtcga
     4081 tgcggtcccc gatgcgctgg tcgacgagat ctcgctgctc ggcccccatg gacgcattcg
     4141 cgagcgcctg caggactgga agaagacgcg cgtgagctcg ctgctgatcg ggcacagcgg
     4201 ggacttcga
//
LOCUS       DAORUQ010000239         4176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_27285_length_4176_cov_6.504247, whole genome shotgun sequence.
ACCESSION   DAORUQ010000239 DAORUQ010000000
VERSION     DAORUQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27285_length_4176_cov_6.504247"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..851)
                     /locus_tag="PLW13_14720"
     CDS             complement(207..851)
                     /locus_tag="PLW13_14720"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HQY71684.1"
                     /translation="MRVRLPWILLLVVPLAVGGAARIDIGRFSAGDLSGWQRKSFRGE
                     TEYTVREEGGVQALCARSRDAGSGLFREREVDLGATPWIHWSWKVDAGLHAGDERTRA
                     GDDYPARVYVVFSGGPAFWRTRAINYVWSSREPEGAVWPNAFTANARMVAVRSGRDGT
                     GKWQSESRDVRADYRRIFGEEPGTADAVAIMTDTDNTGAGAAACYGDIWFSADT"
     gene            complement(1009..3027)
                     /locus_tag="PLW13_14725"
     CDS             complement(1009..3027)
                     /locus_tag="PLW13_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY71685.1"
                     /translation="MSDSIGSFSELNLSAPLLQALADVGYETPSPIQQACIPPLLEGL
                     DLLGEAQTGTGKTAAFALPLLQRLDLKRLEPQILVLTPTRELAIQVAEAFQKYAKHLP
                     GFHVLPIYGGQSMFMQLKQLRRGVHVIVGTPGRVMDHLERKSLELDALQALVLDEADE
                     MLRMGFIDDVEWILEHTPAERQTALFSATMAEQIRRVARTHMREPREVKIKASTRTVE
                     SIRQLYWQVSGTNKIDALTRMLEVDEDLDAAIVFVRTKTATAEIAERLEARGYAAAAL
                     NGDMNQQMRERVIEQLKSGALDIVVATDVAARGIDVARISHVINFDIPYDTEAYVHRI
                     GRTGRAGRQGTAILFVAPREIRMLRTIERVTRAPIEPLSLPSREAVAGRRVAQFRKQV
                     AEVMENEPLDFFADVVDQLGNEHDGDLSRVAAALAYMAQRAKPLRFEARDMAEGFAPA
                     PDRGERDARDARAPRDFRDSRDSRDSRAPREFREPREPRRERPPQIEPGGELVPFRIE
                     VGRMHDVSPREIVGAIANEAGLPSHCIGRIEIYDEFSVVGLPADLPADVLGVLRKTRV
                     MHQPLEIRRAGADDVPPPGKPRERARPSKSERGEGFRSDSRPPRHDAQRPPRGDDYRP
                     PRAAPRAERTGDAPLRRKPAAGAAGPGARDFAPKPRDFAAKPRKPRKP"
     gene            3234..4007
                     /locus_tag="PLW13_14730"
     CDS             3234..4007
                     /locus_tag="PLW13_14730"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HQY71686.1"
                     /translation="MSTTSPSAPQSSPGEVAGWPMLKIRYRTDAAAIAALLPPGIAPG
                     REPMVTLTVYNVPIGGEPEYGVVINVDADYNGIAGEYTLALGIDQEAPLFICQELWGQ
                     PKYPVKVQYFRLGNHIEAKVVHQGCTFIEFQGEVSGTLPNPADHEQNEWWVKSVRGVD
                     PAKCQYDFPPHVVHVWSKYGTAYLQELKGTVTLRESAWDPIATLLPLREQVSARLWTP
                     VFKDRSIKLAGPLDPEGYWPFADTISGTRWPGTSGGPKK"
BASE COUNT          652 a         1475 c         1419 g          630 t
ORIGIN      
        1 tcgacccttc cagtgggccg ccatccctgt gggtcgccat tcatggcgac gacagcaccc
       61 gggccggcac ctgtcggcat gaatgccgac ccacaggcac ttgagccagc agcatacgcc
      121 aagcacctgc gggtcgccat tcatggcgac agcagcacct gggctggcac cagtcggcat
      181 gaatgccgag ccgcagcgat gccggcctac gtgtccgcgc tgaaccagat atcgccgtaa
      241 caggccgccg cgcctgcgcc ggtgttgtcg gtgtcggtca tgatggcgac cgcatcggcg
      301 gtaccgggtt cctcgccgaa gatccggcgg taatcggcgc gcacgtcgcg actctcgctc
      361 tgccacttcc cggtaccgtc gcggcccgag cgcaccgcga ccatccgcgc gttcgcggtg
      421 aaggcgtttg gccagaccgc gccttcgggc tcgcgactgg accagacata gttgatcgca
      481 cgcgtgcgcc agaaagccgg gccgcccgaa aacaccacgt agacgcgcgc ggggtaatca
      541 tcaccggcgc gcgtgcgctc gtcgccggcg tgcaggccgg catcgacctt ccacgaccag
      601 tgaatccatg gcgtggcgcc gaggtccacc tcgcgctcgc ggaacaggcc ggagcccgca
      661 tcgcggctgc gcgcgcagag cgcctgcacc ccgccctcct cccgcacggt gtactcggtc
      721 tcgccacgga atgacttgcg ctgccagccc gacagatccc cggccgagaa gcggccgatg
      781 tcgatgcgcg ccgcgccgcc gacggccagc ggcacgacca gcagcaggat ccacggcagg
      841 cgcacacgca cccccgtgct caggcgcccg ccggccgaag gccatcgcgc ctctcggccc
      901 cgggagtcgg tgcggcaggg catgaacgcg tggcgactgt tcgcgcacca tcatggcgcc
      961 ggcagtggca ggccggcccg gtgaccattg ccagcgcccc gggcatcgtc agggcttgcg
     1021 cggcttgcgt ggcttggcgg cgaaatcccg cggcttggga gcaaaatccc gcgcccccgg
     1081 acccgcggct cccgctgccg gcttgcggcg cagtggcgcg tcgccggtgc gctcggcgcg
     1141 cggcgccgcc cgtggcgggc ggtagtcatc gccccgcggc ggtcgttgcg catcgtggcg
     1201 cggcgggcgc gaatcgctgc ggaatccctc gccgcgctcg ctcttcgacg ggcgcgcgcg
     1261 ctcgcgcggc ttgcccggcg gcggcacgtc gtcagcgccc gcgcggcgga tctccagcgg
     1321 ctgatgcatc acgcgcgtct tgcgcaacac cccgagcacg tcggccggca ggtcggcggg
     1381 caggcccacc acgctgaact cgtcatagat ctcgatgcgc ccgatgcagt ggctcggcag
     1441 ccccgcttcg ttggcaatgg cgccgacgat ttcgcgtggc gacacatcgt gcatgcgacc
     1501 cacctcgatg cgaaacggca ccaactcgcc gccgggctcg atctgcggcg ggcgctcccg
     1561 ccgcggttcg cgcggttccc ggaattcgcg cggtgcacgg gaatcgcggg agtcacgcga
     1621 gtcgcggaaa tcccgcggtg cgcgcgcgtc gcgcgcatcg cgctcgccgc gatcgggcgc
     1681 cggcgcgaaa ccttcggcca tgtcgcgggc ctcgaaacgc agcggcttgg cgcgctgcgc
     1741 catgtaggcc agcgccgccg cgacgcgcga cagatcgccg tcgtgctcgt tgccaagctg
     1801 atcgaccaca tcggcgaaga aatccagcgg ctcgttctcc atcacctcgg cgacctgctt
     1861 gcggaactgc gccacgcgcc gcccggccac cgcctcgcgg ctcggcaggc tgagcggctc
     1921 gatgggcgcg cgcgtcacgc gctcgatggt gcgcagcatg cggatctcgc gtggcgcgac
     1981 gaacaggatc gcggtgccct ggcgtccagc tcggccggta cgaccaatgc ggtgcacgta
     2041 ggcctcggtg tcgtagggga tgtcgaagtt gatcacgtgg ctgatgcgcg ccacgtcgat
     2101 accgcgtgcc gccacgtcgg tggcgaccac gatgtcgagc gcaccgctct tgagctgctc
     2161 gatcacgcgc tcgcgcatct gctggttcat gtcgccgttg agcgccgcgg ccgcatagcc
     2221 gcgcgcctcg aggcgttcgg caatctcggc ggtggccgtc ttggtgcgca cgaacacgat
     2281 cgcggcgtcg agatcctcgt cgacctcgag catgcgcgtc agcgcgtcga tcttgttggt
     2341 gccgctgacc tgccagtaca gctggcggat cgactccacg gtgcgcgtgc tcgccttgat
     2401 cttcacctcg cgcggctcgc gcatgtgggt gcgcgccacg cgccggatct gctcggccat
     2461 cgtggccgag aacagcgcgg tctggcgctc cgccggcgtg tgctcgagga tccactcgac
     2521 gtcgtcgatg aagcccatgc gcagcatctc gtcggcctcg tcgagcacca gtgcctgcag
     2581 cgcgtcgagc tcgaggctct tgcgctcgag gtgatccatc acgcggcccg gggtgcccac
     2641 gatcacgtgc accccgcggc gcagctgctt caactgcatg aacatgctct gcccaccgta
     2701 gatcggcagc acgtggaaac cgggcaggtg cttggcgtac ttctggaagg cctcggcgac
     2761 ctggatcgcg agctcgcgcg tcggcgtcag caccaggatc tgcggctcga gtcgcttcag
     2821 gtccaggcgc tgcagcagcg gcagtgcgaa agccgccgtc ttgccggtgc cggtctgcgc
     2881 ttcgcccagc agatcgaggc cttcgagcag cggcgggatg caggcctgct ggatcggcga
     2941 cggggtttca taacccacgt cggcgagcgc ttgcaacagg ggtgcggaaa ggttcagctc
     3001 gctgaaagag ccaatggaat cggacatgga gtagctaccg gaaatgccgg acctgagcgg
     3061 cgacatgcca gatggcccgg gaggcgccag tatgaaccag tcagggtgtt ttgccgaccg
     3121 ccgcggtgcc gacatactgc cgctttatga aactcctgtc aggttgctcc gtgcggcccc
     3181 ggcggcgggt taccatcacg cagccctcga acaacacgat accgggaacc gccatgagca
     3241 cgacgagtcc gtccgccccg caatccagcc ccggcgaagt cgccggctgg cccatgctga
     3301 agatccgcta ccgcaccgac gcggcagcta tcgccgcgct gctgccgccc ggcatcgcgc
     3361 ccggcaggga gccgatggtc acgctcaccg tttacaacgt gccgatcggg ggcgagcccg
     3421 agtacggggt ggtgatcaac gtagacgccg attacaacgg catcgccggc gagtacacgc
     3481 tggcgctcgg catcgaccag gaggcgccgc tgtttatctg ccaggaactt tgggggcagc
     3541 ccaaataccc ggtgaaggtg cagtacttcc ggctcggcaa ccacatcgag gcgaaggtgg
     3601 tccaccaggg ctgcacgttc atcgagttcc agggcgaggt gagcggcacg ctgcccaacc
     3661 ccgccgacca tgagcagaac gagtggtggg tgaagtcggt gcgcggcgtg gatcccgcga
     3721 agtgccagta cgatttcccg ccgcacgtgg tgcacgtctg gagcaagtac ggcaccgcgt
     3781 acctgcagga attgaagggc acggttacgc tgcgcgagag cgcctgggac ccgatcgcca
     3841 cgctgctccc cctgcgcgag caggtcagcg cacgactgtg gacgccggtg ttcaaggacc
     3901 gcagcatcaa gctcgccggc ccgctggatc cagagggcta ctggcccttc gccgatacca
     3961 tcagcggcac gcgctggccg ggcacctcgg gcgggcctaa aaagtaggtc gggcgccagc
     4021 ccgaccagca aggaatgtag gccgggcttc agcccgggaa gggttcggca aagccgaacc
     4081 cgccatgccc ggcctcaccg caacagtcgg catgaatgcc gacctacacg ggccggtaca
     4141 cccacgcatg tttcggcgcg tgctcggcgc tgccgt
//
LOCUS       DAORUQ010000240         4174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_27314_length_4174_cov_6.657198, whole genome shotgun sequence.
ACCESSION   DAORUQ010000240 DAORUQ010000000
VERSION     DAORUQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27314_length_4174_cov_6.657198"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLW13_14735"
     CDS             complement(<1..277)
                     /locus_tag="PLW13_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQY71687.1"
                     /translation="MILRITAILFPIFAIVALGYWYGRRHDPEMAVANRLNMDIFVPA
                     LVFAALAGRTFTPAEDLPLAAGAVLVLAGCGLLAWPAARLLGLPWKTF"
     gene            397..1590
                     /locus_tag="PLW13_14740"
     CDS             397..1590
                     /locus_tag="PLW13_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011061617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HQY71688.1"
                     /translation="MIIKPKVRGFMCITAHPTGCAASVAEQIAYVRGHGPVANGPKRV
                     LVIGASTGYGLASRITAAFGCGASTIGIFFEKEGADGKCGTAGWYNSAAFHREAEAAG
                     LPAWSINGDAFSTAIKDRTIALIREQLGQVDLVVYSLAAPRRTHPVSGVTHKSTLKPI
                     GKRVEERTLDTDKREIKHVAMDAASEEEIADTVAVMGGEDWEMWIDALSAAGVLAPGA
                     RTTAYTYLGDRITWDIYWDGTIGAAKKDLDRTAVRLNGKLAPLGGKAYVSVLKAVVTQ
                     ASSAIPIMPLYLALLFKVMKARGTHEGCIEQVDGLFRGGLLAENPVLDEAGRLRMDGK
                     ELVPEVQREVEQLWPRVETGNIDELTDFAGYQQEFLRLFGFGFPGVDYDAEVDPVVPI
                     ANLVA"
     gene            1587..2420
                     /locus_tag="PLW13_14745"
     CDS             1587..2420
                     /locus_tag="PLW13_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY71689.1"
                     /translation="MITLEPGRTSELSARVRRIVAPNPSVMTGPGTNTYLVGREQVAV
                     IDPGPEEGSHIEAILAEGAGRIRWILVTHTHEDHSPAAAALARETGAELRGQVAPEDE
                     LYQDRTFRPDVPFSDGAELATPEFRLRAIHTPGHAANHYCVLLEEEGMLMTGDHIMNG
                     STVVIIPPGGDMKDYLDSLEKLKTYPLQHLAPGHGELMSGPVAVIDGIVAHRLQREAK
                     VVRALAATGGGTLDELVLSAYDDVPAALHMMAKYSLWAHLIKLVREGRAVDRAGHWAP
                     G"
     gene            complement(2436..3719)
                     /locus_tag="PLW13_14750"
     CDS             complement(2436..3719)
                     /locus_tag="PLW13_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71690.1"
                     /translation="MHTRSRRIVPLRPPAAVTAPAPERTRADSETHAQLHATFASFYN
                     ALFDAACLANDEQRHARTIQLLRAVKAQENVIIEGICADIARAGAPAEAPVARLLERA
                     LARHCGALALEPGEQALLETACAEQLLAPEQRARAAPAAATDHDSAARLVARLRAVGL
                     ELEPAITTDRADCASPCSTEPAPAPESAPAPTAVAEPRRRLTRGAVMTALLAAALAAV
                     SAAFHEPISRAIRPPAPVAARIQPAPVALAAPVEANETRQAATPPLPAGEPALAPATV
                     TPATEPAPAAPAPAGALPAPETPVVDARIGAATAPERDEAPVASARLATQVSYLLQRG
                     DAALAALRLSEPFADSAAANYSAVLAIDAQNAEARRGLERIVTAYTALARSALQRGDT
                     GYARQLLARARTVVPGSVALQQLEQEIGNAPEAGP"
     gene            3963..>4174
                     /locus_tag="PLW13_14755"
     CDS             3963..>4174
                     /locus_tag="PLW13_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HQY71691.1"
                     /translation="MEVTNLESIPGKTIVRHLGLVQGSTVRAKHAGRDILAGLKNIVG
                     GELKAYTELLVESRKEAVARMVQQAEA"
BASE COUNT          657 a         1391 c         1531 g          595 t
ORIGIN      
        1 cgaaggtctt ccacggcagc ccgagcaggc gcgccgcggg ccaggccagc aggccgcaac
       61 cggcgagcac cagcacggcg ccggcggcga gcggcaggtc ctcggccggt gtgaacgtcc
      121 gccccgcgag cgccgcgaac accagtgccg gcacgaaaat gtccatgttc agacggttcg
      181 cgaccgccat ctcgggatcg tggcggcgtc cgtaccagta gccgagggca acgatcgcga
      241 agatcgggaa caggatcgcg gtaatgcgca ggatcatcgg gacaatccgg aaatgggccg
      301 ccggcagcat atgcgggggc tcggccaaac accacgcctg cggtctataa tgccggccct
      361 gcaagcctgc gccacccacc cgtacaggaa cgaacgatga tcatcaagcc caaggtccgc
      421 ggattcatgt gcatcacggc ccatccgacc ggttgcgcgg ccagcgtcgc cgagcagatc
      481 gcgtacgtgc gtggccatgg ccccgttgcg aacgggccga agcgcgtgct cgtgatcggc
      541 gcctcgacgg gttatggcct ggcctcgcgc atcaccgcgg ccttcggttg cggtgcgtcc
      601 acgatcggca tcttcttcga gaaggaaggc gcggacggca agtgcggcac cgcgggctgg
      661 tacaactcgg cggcgtttca tcgcgaggca gaggccgcgg ggctgccggc gtggagtatc
      721 aacggcgatg cgttttccac cgcgatcaag gaccggacca tcgcgctgat cagggagcaa
      781 ctggggcagg tcgacctggt ggtctacagc ctggccgcgc cgcggcgcac tcatccggtg
      841 agcggcgtga cgcacaagtc cacgctcaag cccatcggca agcgcgtcga ggagcgtacg
      901 ctcgacaccg acaagcgcga gatcaagcat gtcgcgatgg acgcggcgag cgaggaggaa
      961 atcgccgaca ccgtcgcggt gatgggcggc gaggactggg agatgtggat cgatgcgctg
     1021 tccgccgcgg gggtgctcgc accgggtgcg cgcaccaccg cctacaccta cctcggggat
     1081 cgcatcacct gggacatcta ctgggacggc accatcggcg cggcgaagaa ggacctcgac
     1141 cgcaccgcgg tgcggctgaa cgggaagctc gcgccgctcg gcggcaaggc ctatgtctcg
     1201 gtgctgaaag ccgtggtcac gcaggcgagc tccgcgatcc cgatcatgcc gctctacctc
     1261 gcgctgctgt tcaaggtgat gaaggcgcgc ggcacgcacg agggttgcat cgagcaggtc
     1321 gacgggctgt tccgcggcgg gctgctggcc gagaaccccg tgctcgacga ggccgggcgc
     1381 ctgcgcatgg acggcaagga actcgtcccg gaggtgcagc gcgaggtcga gcagttgtgg
     1441 ccccgcgtcg agaccggcaa catcgacgag ctcacggatt tcgccggcta ccagcaggag
     1501 ttcctgcgcc tgttcggttt cggcttcccg ggcgtggatt acgacgccga ggtcgacccg
     1561 gtggtgccga tcgcgaacct cgtcgcatga tcaccctcga gcccggcagg accagcgagc
     1621 tctcggcgcg cgtgcgccgc atcgtcgcgc cgaacccgag cgtgatgacg gggccaggca
     1681 ccaacaccta cctggtcggc cgcgagcagg tcgcggtgat cgacccgggg ccggaggagg
     1741 gctcgcacat cgaggcgatc ctggccgagg gagcgggccg catccgctgg atcctggtca
     1801 cgcacaccca cgaggaccat tcgccggccg cggcggcgct cgcgcgcgag accggcgccg
     1861 agctgcgggg gcaggtcgca cccgaggacg agttgtacca ggaccggacg ttcaggcccg
     1921 acgtgccgtt ctccgacggc gccgagctgg ccacgcccga gttccggctg cgcgcgatac
     1981 acacgcccgg gcacgcggcg aatcactact gcgtgctgct cgaggaagag ggcatgctga
     2041 tgacgggcga tcacatcatg aacggctcga ccgtggtgat catcccgccg ggcggcgaca
     2101 tgaaggatta tctcgactcg ctcgaaaagc tgaagacgta cccgctgcag cacctggcgc
     2161 cggggcacgg cgaactgatg tccgggccgg tggcggtgat cgacggcatc gtcgcgcacc
     2221 gtctgcagcg cgaggccaag gtggtgcgcg cgctggcggc gacgggcggc ggcacgctgg
     2281 acgaactggt gctgtcggcc tacgacgatg tgcccgcggc gctgcacatg atggcgaagt
     2341 attcgttgtg ggcgcacctg atcaagctcg tgcgcgaggg gcgggccgtc gaccgcgcgg
     2401 ggcactgggc gcccggctga tcgcgcttcc agcgcctacg gccccgcctc gggcgcgttc
     2461 ccgatctcct gctcgagttg ctgcaatgcg acggatccgg gcacgaccgt gcgtgcacgt
     2521 gccagcaatt gccgcgcata ccccgtgtcg ccgcgctgca gcgcgcttcg tgccagagcg
     2581 gtgtaagccg tgacgatgcg ctccaggccc cgtcgtgcct cggcgttctg cgcatcgatc
     2641 gccagcacgg cgctgtagtt cgccgcggcg ctgtcggcaa acggctccga gaggcgaagg
     2701 gccgcgagcg cggcatcgcc gcgttgcagc aggtagctca cctgggttgc gagccgcgcg
     2761 ctggccacgg gcgcttcgtc ccgctcgggt gcggtggcgg ccccgattcg cgcatcgacc
     2821 acgggtgtct cgggcgcagg cagtgcgccg gccggcgcgg gggctgccgg tgccggttcc
     2881 gtagcgggcg tgacagtcgc gggggccagg gccggctcgc cggcgggcag cggcggtgtc
     2941 gccgcctgcc gtgtctcgtt tgcctcgacc ggggcggcga gcgcaacggg cgccggctgg
     3001 atgcgcgcgg ccaccggtgc cggcgggcgg atcgccctgc tgatgggctc gtggaacgcc
     3061 gccgacacgg cggccaaggc cgcggcgagc agtgccgtca tgacggcacc ccgcgtgagc
     3121 cgccggcggg gttccgccac cgcggtgggc gccggtgccg attccggtgc cggcgccggt
     3181 tccgtgctgc agggcgatgc gcagtccgcg cgatcggtgg tgatcgcagg ctccagttca
     3241 agcccgacgg cgcgcaggcg cgcgaccagg cgcgcggccg agtcgtgatc ggtggccgcc
     3301 gccggcgccg cgcgcgcgcg ctgctccggc gcgagcagct gttccgcgca ggctgtctcc
     3361 agcagcgcct gctcgccggg ctccagcgcc agcgcgccac agtgccgcgc gagcgcgcgc
     3421 tcgagcaagc gcgccacggg cgcctccgcc ggcgcacccg cgcgcgcgat gtcggcgcaa
     3481 atgccttcga tgatcacgtt ctcctgtgcc ttcacggcgc gcagcagctg gatcgtgcgc
     3541 gcatggcgct gctcgtcgtt ggcaaggcac gccgcgtcga acagcgcgtt gtagaagctg
     3601 gcgaacgtcg cgtgcagctg cgcatgcgtt tcgctgtccg cccgagtacg ctctggcgcc
     3661 ggcgccgtga cggcggcggg cggtcgcagg ggaacgattc ggcgcgatcg tgtgtgcatg
     3721 ggatatataa agcaataatc gggctcatca tgaaaatata ttgagtttca acaagatgaa
     3781 atcgaaggct ttaatttatg cgcgcaggtt gtcaaatatt tcgacacgcc gggtggcggc
     3841 gtggcagcag gggtggcgcg cggcgactat gctgcctgta gcccgcggca gtgcctgagg
     3901 gggggcattg gcgcccgtca ccgcctgcgc gccccgagcg aaacaacgga tggaagcaaa
     3961 ccatggaagt caccaacctc gaatcgattc ccggcaagac catcgtgcgc cacctcgggc
     4021 tggtgcaggg ctcgacggtg cgcgccaagc acgccgggcg cgacatcctc gcgggactga
     4081 agaacatcgt cggcggcgag ctgaaggcct acaccgagct gctggtggag tcgcgcaagg
     4141 aggcggtggc gcgcatggtg cagcaggccg aggc
//
LOCUS       DAORUQ010000241         4165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_27407_length_4165_cov_3.920681, whole genome shotgun sequence.
ACCESSION   DAORUQ010000241 DAORUQ010000000
VERSION     DAORUQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27407_length_4165_cov_3.920681"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PLW13_14760"
     CDS             <1..1333
                     /locus_tag="PLW13_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQY71692.1"
                     /translation="LHRWPQQFVAQERLTPSRLPVFGGEAMVSRPALLRTFAVAAEAS
                     YRVLPGGLTRVGASENASMISNQVGALSKDTWVLATEPDTQALPAPPAPRDGARVLEN
                     TTLPSRVVENLYWMGRYAERAENGLRLMRTVFLQLNSASAMPPQARRILLQAVTRLTS
                     TYPGFFATPELLERPEPELLAVTVDRERAGSIGSCIHALLASAEETKELLSADTQRTI
                     NDIRDHTEQLERTLAGALLSAPEEALDPLVSSLLSFSGIVHESMIRGLGWRFIDMGRR
                     LERAVQTINLSRAILIEQLEESDEAAVLESLLLTIEALISYRRRYRASLNVRDVLELT
                     LIDTTNPRSILYQIERLQQHIAELPGSVSRQLELEGEQRHLLEAVSRIRLSELAALAE
                     ADASSHTRAELDQLFSRVNHLLRETSDQLTGKFFEHARGGQQLIRQNRGFE"
     gene            1333..2214
                     /locus_tag="PLW13_14765"
     CDS             1333..2214
                     /locus_tag="PLW13_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQY71693.1"
                     /translation="MLYRVTHRTRYDYEEIVGQCHNVAFMMPRNTLRQTCLQADVLVE
                     PQPVALTERRDYFGNRVCHFAIEGPHRSLEVTARSLVDVREGGSAGSQLDFGNTCAQV
                     LGMLTEDRGGDTLLAREFVLPSPLVEDGAEIRDYAGPFFAADRPFLSAVRALTQSIFQ
                     EFRYDTTFSDVSTPVGEVLEHRRGVCQDFAHLAIACVRSMGFAARYVSGYLETLPPPG
                     REKLVGSDASHAWFAVYSPGEGWFEFDPTNDQVAGHQHIVTAWGRDYADVAPLRGVVF
                     GGGEKHRLRVSVDVSRV"
     gene            complement(2224..3201)
                     /locus_tag="PLW13_14770"
     CDS             complement(2224..3201)
                     /locus_tag="PLW13_14770"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQY71694.1"
                     /translation="MLSRVAESIYWMARYVERAENTARLVLVNLNVMLDLPRTVRPGW
                     DKLVEIMGSEELFRDLHRRSDERAVTGFLVSERRNPGSMINSLQYARENARTIRDIIP
                     QEAWEQLNSLHQYGQEHAADALVQKRRFDYLHGIIRGAQTLTGILAGTLTHDAGYAFL
                     KAGRNLERADMTTRIIDVRSAGLPGMEGDEALAAYANLQWMSVLKSLTGYQMYRREMQ
                     VRVQRPMVLRFLLQDPHFPRAVRHCLGQAGQCIAQLPRAEAPLKIVRQLDLALEKAEP
                     EALDQAALHAYIDTLQLGMAGIHEAIAATWFRHEPRRRAVRAVAPAPGG"
     gene            complement(3204..>4165)
                     /locus_tag="PLW13_14775"
     CDS             complement(3204..>4165)
                     /locus_tag="PLW13_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQY71695.1"
                     /translation="LEDNLRVPSGVSYMLENRQVMKRVFPELFEDNNILPIDDYPSQL
                     YDMLASLSPRQRARPQIVVLTPGIYNSAYFEHAYLAQQMGAELVEGRDLVVGDDDCVY
                     MRAVEGLVRVDVIYRRIDDMFLDPEAFNPESMLGVPGLLRAWKAGKVALANAPGAGVA
                     DDKVVYAFVPKMIRYYLGEDAILPNVSTYLCVDAADRAYVLANLDKLVVKPANESGGY
                     GMLIGPHASKREREQFAKLIAKDPRNYVAQPMLTLSTAPTLVDSHVEPRHLDLRPFVL
                     SGRDIHVTNGGLTRVALRKGSTVVNSSQGGGSKDTWIVDMGDN"
BASE COUNT          644 a         1448 c         1409 g          664 t
ORIGIN      
        1 cctgcaccgc tggccgcagc aattcgtcgc ccaggaacgg ctgacaccct cgcgcctgcc
       61 ggtattcggc ggcgaggcca tggtgtcgcg cccggcactg ctgcgcacct tcgccgtggc
      121 agccgaggcc tcgtaccggg tgctccccgg ggggctcacg cgggtgggcg cgagcgaaaa
      181 tgcatcgatg atttccaacc aggtcggcgc gctgagcaag gacacctggg tgctggccac
      241 ggagccggac acgcaggcgc tgcccgcgcc gccggcgccg cgcgacggcg cacgggtact
      301 cgagaacacc acgctgccga gccgcgtggt cgagaacctc tactggatgg gacgctatgc
      361 cgagcgcgcc gagaacggcc tgcgcctgat gcgcaccgtg ttcctccagc tcaacagcgc
      421 cagcgcgatg ccgccgcagg cgcgacggat cctgctgcag gccgtgaccc ggctcacgag
      481 cacctatccg ggcttcttcg ccacgccgga gctgctggag cggccggaac ccgagctgct
      541 cgcggtcacc gtggaccgcg agcgcgccgg cagcatcggc agctgcatcc acgcgctgct
      601 ggcgagcgcc gaggaaacca aggaactgct gtcggcggac acgcaacgca cgatcaacga
      661 catcagggac cacaccgagc agctcgagcg caccctcgcc ggcgccctgc tctcggcgcc
      721 ggaagaggcg ctcgacccgc tcgtctcgag cctgctgtcg ttctcgggca tcgtgcacga
      781 gagcatgatc cgcggcctgg gctggcgctt catcgacatg ggccggcgcc tggagcgggc
      841 cgtccagacg atcaacctct cgcgcgcgat cctgatcgag cagctcgagg aatccgacga
      901 agcggctgtg ctcgaatcgc tgctgctgac catcgaggcg ctgatctcct atcgccggcg
      961 ctaccgcgcg tcgctgaacg tgcgcgacgt gctggagctc accctgatcg acaccaccaa
     1021 tccgcgctcg atcctctacc agatcgagcg cctgcagcag cacatcgccg agctgccggg
     1081 cagcgtgtcg cgccagctcg agctcgaggg cgagcagcgc cacctgctcg aggcggtgag
     1141 ccgcatccgc ctgagcgagc tcgcggcgct cgccgaagcg gatgcgtcca gccacacccg
     1201 cgccgagctg gaccagttgt tcagccgcgt gaaccacctg ctgcgcgaga cctcggacca
     1261 gctcacgggc aagttcttcg agcacgcgcg cggcggccag cagttgatac gccagaaccg
     1321 gggattcgag tgatgctcta ccgggtcacg caccgcacgc gctacgacta cgaggagatc
     1381 gtgggccagt gccacaacgt cgccttcatg atgccgcgca acaccttgcg ccagacctgc
     1441 ctgcaggccg acgtgctcgt cgagccgcag cccgtggcgc tcaccgagcg gcgcgactat
     1501 ttcggcaacc gggtttgtca cttcgccatc gagggcccgc accgcagcct ggaggtcacg
     1561 gcccgtagcc tggtcgatgt gcgcgaggga ggcagtgccg gctcgcagct cgatttcggc
     1621 aatacctgcg cccaggtgct gggcatgctc accgaggacc gcggcggcga tacgctgctg
     1681 gcgcgcgaat tcgtgctgcc ctcgccgctg gtcgaggacg gcgcggaaat acgcgactac
     1741 gcgggccctt tcttcgccgc cgaccgtccg ttcctgtcgg ccgtgagggc cctgacgcag
     1801 tcgatcttcc aggaattccg ctacgacacc acgttcagcg atgtcagcac gcccgttggc
     1861 gaggtgctgg agcaccgccg tggcgtatgc caggacttcg cccacctcgc gatcgcctgc
     1921 gtgcgctcga tgggctttgc cgcgcgctat gtctcgggct acctcgaaac cctgccaccg
     1981 ccgggacgcg agaagctcgt gggctccgat gcctcgcacg cctggttcgc ggtgtactcg
     2041 cccggggagg gctggttcga attcgacccg accaacgacc aggtcgccgg ccaccagcac
     2101 atcgtcaccg cgtggggacg cgattacgcc gacgtcgcgc cgctgcgcgg cgtggtcttc
     2161 ggcggcggcg agaagcaccg gctgcgggtc agcgtcgacg tcagccgcgt gtgagcggcg
     2221 ccttcaaccg cccggggcgg gcgcgacggc gcgcacggcc ctgcgcctgg gctcgtggcg
     2281 gaaccaggtc gcggcgatcg cctcgtggat cccggccatg ccgagctgca gcgtgtcgat
     2341 gtaggcgtgc agcgcggcct ggtccagcgc ctcgggctcg gccttttcca gcgccagatc
     2401 gagttgccgg acgatcttca gcggcgcctc ggcacgcggc agctgcgcga tgcactgccc
     2461 ggcctgtccc aggcagtggc gcaccgcgcg cggaaaatgc gggtcctgca gcaggaagcg
     2521 caacaccatc gggcgctgca cccgtacctg catctcgcgg cggtacatct ggtagcccgt
     2581 cagcgatttc agcacgctca tccattgcag gttggcatag gcggccagcg cctcgtcgcc
     2641 ctccatgccg ggcagcccgg ccgagcgcac gtcgatgatg cgcgtggtca tgtcggctcg
     2701 ctcgaggttg cgcccggctt tcaggaaggc gtagcccgca tcgtgcgtca gcgtgcccgc
     2761 gaggatgccg gtcagcgtct gcgccccgcg gatgatcccg tgcaggtaat cgaagcgacg
     2821 cttctgcacc agcgcgtcag cggcgtgttc ctggccgtac tggtgcaggc tgttcagctg
     2881 ctcccaggcc tcctgcggga tgatgtcgcg gatggtgcgc gcgttctcgc gggcgtattg
     2941 cagcgaattg atcatggagc ccgggttgcg ccgttcgctc accaggaacc cggtcacggc
     3001 gcgctcgtcg gagcgacggt gcagatcgcg gaagagctcc tcgctgccca tgatctcgac
     3061 tagcttgtcc cagcccgggc gcaccgtgcg cggcaggtcg agcatcacgt tcaggttcac
     3121 gaggaccagg cgcgcggtgt tctcggcgcg ctcgacgtag cgcgccatcc agtaaatgct
     3181 ctcggcaacg cgtgacagca tgctcagttg tctcccatgt cgacgatcca ggtgtccttg
     3241 ctgccgccgc cctgcgagga gttgaccacg gtggagccct tgcgcaaggc cacgcgcgta
     3301 aggccgccat tggtcacgtg gatgtccctg cccgagagta caaacggcct gaggtccagg
     3361 tgtcgcggct cgacgtgcga gtcgaccagc gtcggcgcgg tcgagagcgt gagcatgggc
     3421 tgggcgacgt agttgcgcgg atccttcgcg atgagcttcg cgaattgctc gcgctcgcgc
     3481 ttgctcgcgt gcgggccgat cagcatgccg tagccgccgg actcgttggc cggcttgacc
     3541 accagcttgt cgaggttggc gagcacgtag gcacgatccg cggcgtccac gcacaggtag
     3601 gtggacacgt tgggcaggat cgcgtcctcg ccgaggtagt agcggatcat cttcggcacg
     3661 aaggcgtaga cgaccttgtc gtcggccacg ccggcgcccg gcgcgttcgc cagcgcgacc
     3721 ttgcccgcct tccaggcgcg cagcaggccg ggcacgccca gcatggattc ggggttgaac
     3781 gcctcgggat cgaggaacat gtcgtcgatg cgccgataga tcacgtcgac gcgcaccagc
     3841 ccctcgaccg cgcgcatgta cacgcaatcg tcgtcgccga cgaccaggtc gcggccctcg
     3901 accagctccg cgcccatctg ctgcgccagg taggcgtgct cgaaataggc cgagttgtag
     3961 ataccgggcg tcagcaccac gatctgcggt cgcgcgcgct ggcgcggtga cagcgaggcc
     4021 agcatgtcgt agagctgcga gggatagtcg tcgatcggca ggatgttgtt gtcctcgaac
     4081 agctccggga acacgcgctt catgacctgg cggttctcga gcatgtagga cacgcccgac
     4141 ggcacccgca ggttgtcctc cagca
//
LOCUS       DAORUQ010000242         4160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_27466_length_4160_cov_3.657491, whole genome shotgun sequence.
ACCESSION   DAORUQ010000242 DAORUQ010000000
VERSION     DAORUQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27466_length_4160_cov_3.657491"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1090
                     /locus_tag="PLW13_14780"
     CDS             200..1090
                     /locus_tag="PLW13_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQY71696.1"
                     /translation="MRLLHSLLVVMLTTTLAGCSMFSKEDEVPPDQGERALYDTARKN
                     LDNSNFDLAIKNLQLLEARYPFGPYAEQAQLELIYAHYRNYDHEEAVAAADRFIRLHP
                     QHPNVDYAYYMKGLASFTEAQGLLERFLPTDMTKRDPGPARQSFADFAQLLSRFPNSE
                     YAADARARMVHLRNLLARYEINVANYYFKRRAHVAALNRGRYVVENFQETPAVPDGLA
                     VMAQAYIILGMPDLADNAIATLRRNYPQHPSLDEAGNFLAQYVPGEDDRSWANVLTFG
                     LVDRAAPPVFDNRDLFDNAP"
     assembly_gap    1335..1434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1435..2627)
                     /locus_tag="PLW13_14785"
     CDS             complement(<1435..2627)
                     /locus_tag="PLW13_14785"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQY71697.1"
                     /translation="MAQVNPLVGDIDGNAAAIVARSREAVAAHGAQVVVFPELALTGY
                     PPEDLLLRTSIALRVERALERLRRELPDAWVLVGYPRRSERGLENVAGLLKGGELVAE
                     YAKMELPNYQVFDEKRYFVAGEQPCVLGIEGFRVALSVCEDIWHERPTALAVAAGAEL
                     VLNINASPFHLGKHLERRKIVERRAIAGGVGVIYVNQVGGQDELVFDGGSMAIGRDGT
                     VAALAPHFEEGLYPVRITRDARGHVAMLSGAVAAEPNLHATVYAALVTGVRDYVNKNR
                     FKGVVLGLSGGIDSALTLAVAVDALGPQRVEAVMMPFRYTAAMSIEDAQAQAATQGVG
                     FKIIPIGPMFDAFMGALDEEFAGRAVDTTEQNIQARCRGVLLMAISNKKGFLVLTTGN
                     KSEMAV"
     gene            2983..3228
                     /locus_tag="PLW13_14790"
     CDS             2983..3228
                     /locus_tag="PLW13_14790"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HQY71698.1"
                     /translation="MLLFRLILLFAMAFIAWRLYRLVVAPRVRASEQPPKLDGEDMVE
                     CALCSVHVPRAQAVSEASRWYCCEEHRREHTESSTGT"
     gene            3232..>4160
                     /locus_tag="PLW13_14795"
     CDS             3232..>4160
                     /locus_tag="PLW13_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71699.1"
                     /translation="MATSLPVTPDSGDPLLRIYAWYRFALAILLLLGYFAFSLGRTEA
                     VFLPLLYLGTAIPYAVLNLAVLIGVLVRPRHPRPRHLFLILVLDIAALVLITHASGGI
                     SSGFAILLMVTVAAAAIFVPGQIATLVAAVASLAVLADATALVLMRDNELSTLLPAGL
                     LGMLLFISSLLMQRLAARLRLSQQLATQKAAEVIELQQLNQLIVQRMRTGIMFVDAQD
                     RVRIANESAALLLGIDRHELAQGGGVPLRLPRVVQDLLELWRANPQRAEAPLRLRQNG
                     PLVQLAFTRLQGPSGDGTLIFAEDFSQIAQQAQ"
BASE COUNT          659 a         1461 c         1282 g          658 t
ORIGIN      
        1 ggcgcagccc ggcgtcttgc ccggtgacaa ggacgttgcg cgtgatcgat tccatggtcg
       61 tttgctgtgg cccggagcgg agcgccccgg gcgagcgcgc gagtctagca cgcacccggg
      121 ggcgcgcttt ggcgggcgcg gcgccggtgt ggttaaatac gccgcctgca aagctccaag
      181 cccttcaagc ggaacaccga tgcgattgct ccattccctg ctcgtcgtga tgctgaccac
      241 caccctggcc ggctgctcca tgttcagcaa ggaggacgag gtgccgccgg accagggcga
      301 gcgcgcactc tacgacacgg cacgcaagaa cctcgacaac agcaacttcg atctcgcgat
      361 caagaacctg cagctgctcg aggcccgtta ccccttcggc ccctacgccg agcaggcgca
      421 gctggagctg atctacgcgc actaccgcaa ctacgaccac gaggaagccg tggccgcggc
      481 cgaccgcttc atccgcctgc acccgcagca cccgaacgtc gactacgcct actacatgaa
      541 gggcctggca tccttcaccg aggcccaggg actgctggaa cgtttcctgc cgacggacat
      601 gaccaagcgc gaccccggcc cggcgcggca gtccttcgcc gatttcgcgc aattgctgtc
      661 gcgcttcccg aattccgagt acgcggccga cgcgcgcgcg cgcatggtgc acctgcgcaa
      721 cctgctggca cgctacgaga tcaacgtggc caactactac ttcaagcgtc gcgcccatgt
      781 cgccgcgctg aaccgcggac gctacgtggt ggagaatttc caggagacac cggcggtgcc
      841 ggacggcctg gcggtgatgg cgcaggccta tatcatcctc ggcatgccgg atctcgccga
      901 caacgcgatc gccacgctgc gccgcaacta cccgcagcat ccctcgctgg acgaagcggg
      961 caacttcctc gcgcagtacg tacccggcga ggacgaccgc tcgtgggcca atgtgctcac
     1021 cttcggcctg gtcgatcgcg ccgccccgcc cgtgttcgac aaccgcgacc tgttcgacaa
     1081 cgcaccgtag gtccggattc atccggacaa ttgccctccg tgggtccgga ttcatccgga
     1141 caatttgtgg gttcggcttc agccgaacga tgtccggata aatccggacc cacagatcaa
     1201 tccccggcct gccagcccga tgtcaccggg tagcgccggt cgcgcccgaa ggccttgtgg
     1261 gtgatgcgcg gaccgatggg cgcctgccgg cgcttgtact cgttcagatc gaccatgcgc
     1321 accacgcggc gcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccgcc
     1441 atctcgctct tgttgcccgt ggtgagcacc aggaagccct tcttgttgga gatcgccatc
     1501 agcagcacgc cgcggcagcg tgcctggatg ttctgctcgg tggtgtcgac ggcgcggccc
     1561 gcgaactcct cgtccagcgc gcccatgaag gcatcgaaca tcggcccgat cgggatgatc
     1621 ttgaagccca cgccctgcgt cgcggcctgt gcctgggcat cctcgatgct catcgccgcg
     1681 gtgtagcgga acggcatcat caccgcctcg acgcgctgcg gccccagcgc atccaccgcc
     1741 acggccagcg tgagcgccga atcgatgccg cccgagagcc ccagcaccac gcccttgaag
     1801 cggttcttgt tcacgtagtc gcgcacaccg gtgaccagcg ccgcgtagac cgtggcgtgc
     1861 aggttcggct ccgcggcgac cgcgcccgac agcatcgcga cgtgcccccg ggcatcgcgc
     1921 gtgatgcgca ccgggtagag gccttcctcg aaatgcggcg ccagcgcggc caccgtgccg
     1981 tcgcgcccga tcgccatcga accgccgtcg aacaccagct cgtcctggcc gccgacctgg
     2041 ttgacgtaga tcacgccgac gccgccggcg atggcgcgac gctccacgat cttgcgccgc
     2101 tcgagatgct tgcccagatg aaacggcgag gcgttgatgt tcagcaccag ctccgcgccc
     2161 gcggccaccg cgagcgccgt cgggcgctcg tgccagatgt cctcgcacac cgacagcgcg
     2221 acgcgaaagc cctcgatccc gagcacgcag ggctgctcgc cggccacgaa atagcgcttc
     2281 tcgtcgaaga cctggtaatt cggcaattcc atcttggcgt actcggcgac gagctcgcca
     2341 cccttgagca ggcccgcgac gttctcgagt ccgcgctcgc tgcgccgcgg ataaccgacc
     2401 agcacccacg cgtcgggcag ctcgcgccgc aggcgctcca gcgcgcgctc gacgcgcagc
     2461 gcgatgctgg tgcgcagcag caggtcctcg ggcgggtagc cggtgagcgc gagctcgggg
     2521 aacaccacca cctgtgcccc gtgcgcggcc accgcctcgc ggctgcgcgc gacgatcgcg
     2581 gcagcgttgc cgtcgatgtc cccgaccagc gggttgacct gcgccatgac cacgatcagc
     2641 tgcgtcacgt tgaactctcg actgcgatgg aacggtgagg cggcattgtc acgcaatcgg
     2701 cccgccacag gcaaagcggc acggtggatc ctgatcctgt gggtcttcat tctgtgggtc
     2761 cgaatttatt cggacaggtg ccttccccag agcacctgtc agcatgaatg ctgacccaca
     2821 gggagcttgg gccactgcac atgtccggct gaagccggac ccgcacctcc ctgtgggtcc
     2881 gaatttattc ggacaggtgc cttccccgag gcacttgtca gcatgaatgc tgacccacag
     2941 ggactagaat aaccacgagg tgcttccaca aggacaaacc ccatgctgct gttccgcctg
     3001 atactgctgt tcgcgatggc gttcatcgcc tggcgcctct accgcctggt ggtggcgccg
     3061 cgcgtgcgtg cgagcgaaca gccaccgaag ctcgacggcg aagacatggt ggagtgcgcg
     3121 ctctgctcgg tgcacgtgcc gcgcgcccag gcagtgagcg aggcctcgcg ctggtactgc
     3181 tgcgaggaac accgccgcga gcacacggaa agcagtaccg gaacctgagc cgtggcgacg
     3241 agcctccccg tgacgcccga cagcggcgat ccgctgctca ggatctacgc ctggtaccgc
     3301 ttcgcgctgg caatcctgct gctgctgggc tatttcgcgt tcagcctggg gcgcaccgaa
     3361 gcggtgttcc tgccgctgct ttacctgggc accgccattc cctacgcggt gctcaacctc
     3421 gcggtactga tcggcgtgct ggtccgcccg cgccacccgc gaccgcgcca cctgttcctg
     3481 atcctggtgc tggacatcgc ggcactggtg ctgatcaccc acgccagcgg cggcatcagc
     3541 agcggtttcg cgatcctgct gatggtcacg gttgccgccg cggcgatctt cgtgcccggg
     3601 cagatcgcca cgctggtcgc cgcggtcgcc agcctcgcgg tgctcgcgga cgccaccgcg
     3661 ctggtgctca tgcgcgacaa cgagctgtcg acgctgctgc ccgccggcct gctcggcatg
     3721 ctgctgttca tcagctcgct gctgatgcag cggctcgcgg ctcgcctgcg cctcagccag
     3781 cagctggcga cgcaaaaggc cgcggaagtc atcgagctgc agcagctcaa ccagctgatc
     3841 gtgcaacgca tgcgcaccgg tatcatgttc gtcgacgcgc aggaccgcgt gcgcatcgcc
     3901 aacgaatcgg ccgcgctgct gctcggcatc gaccgccacg agctcgccca gggcggcggc
     3961 gtgccgctgc ggctgccgcg cgtggtgcag gacctgctgg agctgtggcg cgcgaacccg
     4021 cagcgcgcgg aagcgccgtt gcgcctgcgc cagaacggac cgctggtgca gctcgcgttc
     4081 acacggctgc agggtcccag cggcgacggc acgctgatct tcgccgagga cttctcgcag
     4141 atcgcgcagc aggcgcagca
//
LOCUS       DAORUQ010000243         4153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_27534_length_4153_cov_5.567594, whole genome shotgun sequence.
ACCESSION   DAORUQ010000243 DAORUQ010000000
VERSION     DAORUQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27534_length_4153_cov_5.567594"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLW13_14800"
     CDS             complement(<1..585)
                     /locus_tag="PLW13_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQY71700.1"
                     /translation="MKPAPFDYVAPATIDEACAVLAQAGGGATVLAGGQTLMPLLNLR
                     MSQPFMVVDITRIAELQGIRRADGGTRIGSVTRQCELIANEVLARDLPVLVQAVRHVG
                     HHQTRNRGTVGGSIALGEPAAELPAAALALGATIEVRSTRGTRRIPAAEFYLGPYMTT
                     LEADELVTGITFPDWPPGHVTLFCELAQRPGDFAL"
     gene            complement(582..1349)
                     /locus_tag="PLW13_14805"
     CDS             complement(582..1349)
                     /locus_tag="PLW13_14805"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HQY71701.1"
                     /translation="MNMTGEQRIAAPRARVWEALNDPEILRASIPGCQSLEKVAHDRF
                     TATVEVKVGPIGARFKGAVSLADLDPPYGYTLILDGNGGIAGSVKGSAKVRLNEQAGA
                     TLIPYEVQAQVGGRMAQLGGPIIDATAKQLAGKFFSRFGEIVGAEAIPVPPLAPQAAP
                     VAAPAMPTAPAVAPAGGVPVAWILATVVAALVGFLIGRGQGAAGSDWAGLSIGLLLIV
                     VAGAAFEYGRRAAAPVLVLDNALLRRLIANAKEDAKE"
     gene            complement(1387..3474)
                     /locus_tag="PLW13_14810"
     CDS             complement(1387..3474)
                     /locus_tag="PLW13_14810"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HQY71702.1"
                     /translation="MKPHRVIALLLSGVLAGLTVAVLAGAPDGYPANAGAAANWASHG
                     GGVDESGYSRLEAISSRNIERLGLAWSLDLPGEVTLEATPLAVDGVLYFSGSYSGVYA
                     VDGSSGKLLWKYDPEIWRVAPAKQGQGMPVHRGVAYDNGRVFIGTLDGRLVALDAKTG
                     TPQWTVDTLPANTFHSITGAPRTFNGKVIIGNGGADAAQRGFVTAYEQSTGKQLWRFH
                     TTPGKPEDNAGDAAMEMAAKTWGGEYWKVGGGGGTVWNGITFDPEFNRIYLGTGNGGP
                     YNPRVRSPGGGDNLFLASIVALDADTGKYVWHYQVNPGESWDYKATANMITATVTIDG
                     RPRKVLMQAPTNGFFYVIDRETGKLISAEKTGKVTWAERIDLATGRPVEAPDIRYETG
                     ETIMWPSMLGTHNWQDMSYNPLTGLVYIPYMHLGARYSTNVKPGEFSFGGMTVNYHKA
                     DAEDGTGAVLAWDPVAQRPRWKAPLETIWNGGTLSTAGNLVFHGAGDGWFTAYDATDG
                     RRLWRFDAGLGIVGAPISYAVGDTQYVSVLVGYGGTTAALSGVLNAGWKYGAQPRRLL
                     TFALDGRESLPPSAPRDFTVNAVDDPSLVLDEKAVAEGRALSVMCIACHGADLVGAGS
                     PGPDLRESQLALSAESLWSVLHDGSLAPRGMPAFPMLSRAQVNSLQAAIRAAARESLG
                     TRKSGEQGGVPRL"
     gene            complement(3597..>4153)
                     /locus_tag="PLW13_14815"
     CDS             complement(3597..>4153)
                     /locus_tag="PLW13_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71703.1"
                     /translation="AEAEIQGVDFDVLIAPTESLSITFAYAYIDAEYKEWDREIAAGV
                     VIDYTESPFVYIAEHSLTSSLRYTLPLDASLGEVSLMGSVYWQDDTEASPDAWRWDTL
                     GWSKANLDAALASTVVDDYAIWNFRADWLGVMGSSFDLAAFVNNAADEDYVTGGLNVP
                     DTLGWAAANYGPPRTYGASVRYSF"
BASE COUNT          643 a         1444 c         1381 g          685 t
ORIGIN      
        1 cagcgcgaag tcaccgggac gctgcgccag ctcgcaaaac agcgtgacgt ggcccggcgg
       61 ccagtcggga aacgtgatgc ccgtcaccag ctcgtcggct tcgagcgtgg tcatgtaggg
      121 gccgagatag aactcagcgg cagggatccg gcgcgtgccg cgggtcgagc gcacctcgat
      181 cgtcgcgccc agtgcgagcg cggcggcggg cagctccgcc gccggctcgc cgagcgcgat
      241 cgagccgccg accgtgccgc ggttgcgcgt ctggtgatgc ccgacgtggc gcacggcctg
      301 caccagcacc gggagatcgc gcgccagcac ctcgttggcg atcagctcgc attgccgcgt
      361 gaccgacccg atgcgcgtgc cgccatcggc acgacggatg ccctgcagtt ccgcgatccg
      421 ggtgatgtcg acgaccatga atggctggct catgcgcagg ttgagcagcg gcatcagcgt
      481 ctgcccgccg gccagcacgg tcgcgccgcc gccggcttgc gcgagcacgg cgcaggcttc
      541 gtcgatggtg gccggagcca catagtcgaa gggagcgggc ttcattcctt cgcgtcctct
      601 ttcgcgttgg cgatcagtcg ccgcaacagg gcgttgtcga gcacgagcac cggcgccgcc
      661 gcgcgccgcc cgtattcgaa cgcggcaccg gccaccacga tcagcagcag gccgatggac
      721 agtccggccc agtcggagcc ggcagcgccc tgcccgcggc cgatgagaaa gcccacgagc
      781 gccgcgacaa ccgtcgccag tatccaggcc acgggcaccc cccccgcggg tgcgaccgcg
      841 ggagccgtgg gcatcgccgg tgcagcgacc ggggcggctt gcggtgccag cggcggcacg
      901 ggtatcgcct cggcgccgac gatctcgccg aatcggctga agaacttgcc cgccagctgc
      961 ttcgcggtgg catcgatgat cgggccaccg agctgggcca tgcgcccccc gacctgggcc
     1021 tgcacttcat aggggatcag cgttgcgccg gcctgctcgt tcagccgcac tttcgcgctg
     1081 cccttgaccg agccggcaat gccgccgttg ccgtccagga tgagcgtgta gccgtagggc
     1141 ggatcgaggt cagccagcga taccgcgccc ttgaagcgcg cgccgatggg gccgaccttc
     1201 acctccaccg tcgcggtgaa gcgatcgtgc gccacctttt ccaggctctg gcagcccggg
     1261 atgctggcgc gcaggatctc ggggtcgttc agcgcttccc agactcgcgc gcgcggggca
     1321 gcaatgcgct gctctccagt catgttcata tcgatcgatc ctcggacaat tgagaaggct
     1381 cgcccgtcac aaacgcggga ctccgccctg ctcgccggac ttgcgcgtgc cgagcgattc
     1441 gcgggcagcg gcgcggatgg cggcttgcag gctgttcacc tgcgcgcggc tcagcatcgg
     1501 gaaagccggc atgccgcgcg gcgccaggct gccatcgtgc agcacgctcc acaggctttc
     1561 cgcggacagg gcgagttgcg attcccgcag gtccggcccc ggcgatcccg cgccgaccag
     1621 gtccgcgccg tggcaggcga tgcacatgac cgacagcgcg cgaccctcgg ccaccgcctt
     1681 ctcgtcgagc accagcgagg gatcgtccac ggcgttcacg gtgaaatcac gcggggcgga
     1741 cggcggcagg ctctcgcggc catccagtgc aaaggtcagc agccgacggg gctgcgcgcc
     1801 atatttccag ccggcgttga gcacgccgga cagcgccgcc gtcgtcccgc cgtagccaac
     1861 cagcaccgag acatattgcg tgtcgccgac cgcatagctg atcggcgcgc cgacgatgcc
     1921 gagccccgcg tcgaaacgcc acagccggcg gccatcggtg gcgtcatagg ccgtgaacca
     1981 gccatcgccc gcgccgtgga agacgaggtt tccggcggtg ctcagcgtgc cgccgttcca
     2041 gatcgtctcc agcggtgcct tccagcgcgg gcgctgcgcc accggatccc aggccagcac
     2101 cgctccggtc ccgtcctcgg catcggcctt gtggtaattc accgtcatcc cgccgaagct
     2161 gaactcgccc ggcttcacgt tggtggaata gcgcgcaccg agatgcatgt aggggatgta
     2221 gaccagcccg gtgagcgggt tgtacgacat gtcctgccag ttgtgcgtgc ccagcatgct
     2281 gggccacatg atcgtctcgc cggtttcata gcggatgtcc ggcgcttcca ccggacggcc
     2341 cgtcgcgagg tcgatgcgct cggcccaggt gaccttgccg gtcttttccg ccgagatcag
     2401 cttgcccgtc tcgcgatcga tgacatagaa aaagccgttg gtgggtgcct gcatcagcac
     2461 cttgcgcggg cgcccgtcga tcgtcaccgt ggcggtgatc atgttggcgg tggccttgta
     2521 gtcccaggat tcgccgggat tgacctggta gtgccagaca tatttgccgg tatcggcgtc
     2581 gagcgcgacg atcgaggcga ggaacaggtt gtcgccaccg ccggggctgc gcacgcgcgg
     2641 gttgtagggg ccgccgttgc cggtgccgag atagatgcgg ttgaactccg gatcgaaggt
     2701 gatcccgttc cacaccgtgc cgccgccacc gccgactttc cagtattcgc cgccccaggt
     2761 cttcgcggcc atttccatgg ccgcatcgcc cgcgttgtcc tcgggcttgc cgggggtggt
     2821 atggaagcgc cagagctgct tgcccgtgga ctgctcatag gcggtgacaa agccgcgctg
     2881 ggccgcatcc gcgccgccgt tgccgatgat caccttgccg ttgaaggtgc gcggcgcgcc
     2941 cgtgatcgaa tggaaggtgt tcgcaggcag ggtatcgacc gtccactgcg gcgtgccggt
     3001 cttcgcgtcg agcgccacca gcctgccgtc gagcgtgccg atgaacaccc ggccattgtc
     3061 gtaggcaacg ccgcgatgca ccggcatgcc ctgtccctgc ttggccggcg caacgcgcca
     3121 gatctcggga tcgtatttcc acagcagctt tccgctcgag ccgtcgacgg catagacccc
     3181 cgaatagcta ccggagaaat agagcacgcc gtccaccgcc agtggggtcg cctccagcgt
     3241 gacctcgccc ggaagatcca gcgaccaggc gaggcccagg cgctcgatgt tacggctgct
     3301 gatggcctcg agccggctgt agccgctttc gtccaccccg ccgccgtggc tggcccagtt
     3361 cgccgcggcg ccggcattgg ccggataccc gtctggggcc cccgcgagga ccgcaacagt
     3421 caaccctgcc aggacgcccg agagcagcag tgcgatcact ctgtgcggtt tcatgtcggc
     3481 tgtgctctct tttcgttatg tcggatgcaa acggttgccc cgcgattgta ggaaccctgc
     3541 ccccgggtgt cactcctctg acacgaattt aattgtgcgc gccggcggca gcgcgatcag
     3601 aagctgtaac gcaccgatgc cccgtaggtg cggggcgggc cgtagttcgc ggctgcccag
     3661 cccagggtgt ccggcacgtt cagtccgccg gtgacgtagt cctcgtcggc cgcgttgttg
     3721 acgaaggccg cgagatcgaa actcgagccc atgaccccga gccagtcggc gcggaaattc
     3781 cagatcgcgt agtcatccac gaccgtcgac gccagggccg cgtcgaggtt cgccttgctc
     3841 cagccgagcg tgtcccagcg ccacgcgtcg gggctggcct ccgtatcgtc ctgccagtag
     3901 acgctgccca tcagcgatac ctcgccgagc gacgcgtcca gcggcagcgt gtacctgagc
     3961 gaactcgtga gcgagtgctc cgcgatatag acgaacggcg attccgtgta gtcgatgacc
     4021 accccggccg cgatctcgcg atcccactcc ttgtactcgg catcgatata agcgtaagcg
     4081 aacgttatgc tcagcgactc ggtcggggcg atcagcacgt cgaaatcgac accctgtatc
     4141 tcggcctcgg cgg
//
LOCUS       DAORUQ010000244         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_28196_length_4087_cov_6.510417, whole genome shotgun sequence.
ACCESSION   DAORUQ010000244 DAORUQ010000000
VERSION     DAORUQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28196_length_4087_cov_6.510417"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLW13_14820"
     CDS             <1..405
                     /locus_tag="PLW13_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY71704.1"
                     /translation="SSIREGAYALGATRWQMVRDHIIPYSTGGILTGVIIGLARAIGE
                     TAPIITIGALTFIAFLPEPPISAQFPFVSFEWLKSPFTVMPIQMFNWMSRPQAAFQEN
                     AAAAGVVLVVMTLVMNGAAIWLRLRYRKRIKW"
     gene            513..1250
                     /gene="pstB"
                     /locus_tag="PLW13_14825"
                     /pseudo
     CDS             513..1250
                     /gene="pstB"
                     /locus_tag="PLW13_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            1274..1999
                     /gene="phoU"
                     /locus_tag="PLW13_14830"
     CDS             1274..1999
                     /gene="phoU"
                     /locus_tag="PLW13_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQY71705.1"
                     /translation="MGKDEIGQHTVQQYNEDLENLHRGVLDMGQLVLAQLDDGIAALE
                     GNDKELATRVVRRDVEVNTLEVHLDKECQRILVRRQPAAGDLRLIFAVIKTVTDLERI
                     GDQVGHVARAVLESDLSSPNRPHAALAHLAGMVRTNCASAMDSFARLDAAMALEVVQA
                     DVEVNREYQGSMRQLLTYIMEDPRITSSALQLAWTARALERIGDHAKNIAEYVIYMVH
                     GEDVRHISEEERRQRLASEAPSV"
     gene            2439..2514
                     /locus_tag="PLW13_14835"
     tRNA            2439..2514
                     /locus_tag="PLW13_14835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2472..2474,aa:Val,seq:tac)
     gene            2533..2609
                     /locus_tag="PLW13_14840"
     tRNA            2533..2609
                     /locus_tag="PLW13_14840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2567..2569,aa:Asp,seq:gtc)
     gene            complement(2889..>4087)
                     /locus_tag="PLW13_14845"
     CDS             complement(2889..>4087)
                     /locus_tag="PLW13_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71706.1"
                     /translation="PARWLALFCALLAGGYGVMVTAVSSPLFDGYLQAVVLLQRNGVD
                     VALLRGTGHAVAALERLLWIAGWTFAGAVAIHFVRSVLGIAASPRPHIAAQAALIQLS
                     FACVALTLPRTAAITATVLSLAVAGLLLWRRAPPPARAGSSWLLASATLFTLYEGSGL
                     ARAVDALPIELALAALLLLAAAWRVAEARPGLLFTLPGLLALATALRIEAVPGALLVM
                     NLWLAVCMIVLVTTIARNLRALDADGRRQALCFLSGWVLAATLAAAWQLLVWGGRLAG
                     CTPAGESALCWLGRYQDWLLVAPLPILLLSFVAGLLHRGPIDAARLFRRTAVYGSMLL
                     LSLFVLGAIETLLGDFLRRGLPGETPPLVAAGVMGVVFYPAKRACDRGVERFLARLLA
                     WADGAS"
BASE COUNT          769 a         1345 c         1382 g          591 t
ORIGIN      
        1 tcgagcatcc gcgagggcgc ctacgcgctg ggggccacgc gctggcagat ggtgcgcgac
       61 cacatcatcc cgtattccac cggcgggatc ctgaccggcg tgatcatcgg gctggcgcgc
      121 gccatcggcg agacggcacc gatcatcacg atcggcgcgc tgaccttcat cgccttcctt
      181 cccgagccgc cgatcagcgc gcagtttccc ttcgtatcct tcgagtggct gaagtcgccg
      241 ttcacggtga tgccgatcca gatgttcaac tggatgtcgc gcccccaggc cgcgttccag
      301 gaaaacgccg cggcggccgg cgtggtgctg gtggtgatga cgctggtgat gaacggggcc
      361 gcgatctggc tgcgcctgcg ctacaggaaa cgcatcaaat ggtgagcgaa atgaacgacg
      421 cgagcatggg caggaacatg aacatgacgg cgggatcgct tcccccgggc gtgagcacac
      481 cggggcagag cctcaaggcc gaggcacgtg cgctggattt ttactacggc gaggtgcagg
      541 cgctgcggag cgtcaccatg ccgatctggg aacacaaggt cacggcgctg atcggtccct
      601 cgggctgcgg caagtcgacc ttcctgcgct gcttcaaccg catgcacgac ctcggggagg
      661 tacgcaaccg ttatggcggc gagatcgtgt tccagcccga cggagtgaac atcctggcca
      721 agtcggtgga cccgatcgag gtgcgcatgc gcatcgggat ggtgttccag aagcccaatc
      781 cctttccgaa gtccatcttc gaaaacattg cctacgggct gcgggtgcgc ggcgagcgca
      841 accggagcgc gatggccgac aaggtcgagc aggcgctgaa ggatgcctcg ctgtgggaag
      901 aggtcagcga ccgcctgcag gaccaggcct tcaacctctc gggcgggcag cagcagcgat
      961 tgtgcatcgc gcgcgcgctg gccacggccc ccgagctgct gctgttcgac gagcccacct
     1021 cggcgctcga tcccaacgcc accgcgaaca tcgaggacct gatcaaccgc ctcaaggaca
     1081 aggtctcgat cctgatcgtc acgcacaaca tgcagcaggc cgcgcgcgtt tccgactata
     1141 ccgcgttcat gtacctgggc gagctggtcg agttcggcga caccaacacg gtgttcacca
     1201 acccgaggag caagcagacc gaagactaca tcaccggccg cttcggctga tcaggggagg
     1261 ctggatcatg gtcatgggca aggatgaaat cggtcagcac acggtgcagc agtacaacga
     1321 ggacctcgag aacctgcacc ggggcgtgct ggacatgggg cagctggtgc tggcgcaact
     1381 cgacgacggg attgccgcgc tggagggcaa cgacaaggag cttgccacgc gcgtggtgcg
     1441 ccgcgatgtg gaggtcaaca cgctcgaggt gcatctcgac aaggaatgcc agcgcatcct
     1501 ggtgcgccgc cagcccgccg ccggcgacct gaggctgata ttcgcggtca tcaagaccgt
     1561 gacggacctg gagcgcatcg gtgaccaggt cgggcacgtg gcgcgcgcgg ttctcgaatc
     1621 cgacctgagc agccccaacc ggccgcatgc cgcgctcgcg cacctggcgg gcatggtacg
     1681 cacgaactgc gccagcgcca tggattcgtt tgcgcgcctg gacgcggcga tggcgctgga
     1741 ggtcgtgcag gccgacgtcg aggtcaaccg cgagtaccag ggcagcatgc gccagctgct
     1801 gacctacatc atggaagacc cgcgcatcac ctcctcggcg ctgcagctcg cgtggaccgc
     1861 gcgtgcgctg gagcgcatcg gggatcacgc caagaacatc gccgagtacg tgatctacat
     1921 ggtccacggc gaagacgtgc gccacatcag cgaagaggag cgccgccagc gcttggcgtc
     1981 ggaagctcca tcggtttgag ggcagggcgg ccctggtgcc gccggcgctg cgcagcgatg
     2041 cggcgaggcg atcggccccg ccgagttgtg cacagattct gtggataaaa cggtgcaaaa
     2101 cttgtcggtg tcagcctccg caaggccatg catgcgggtc cggatcgatc gatcaaaaaa
     2161 cgtactaaca agtaaaatca tataaaacaa tgtgttggat tgcattcgtg agatatcgcg
     2221 cgcactatga actgcaatat attgtgcgcg ccgtatcgag tggcgcatgt tgataaggat
     2281 cgttgacgta gcgcccccgc gacgggaagc cggcgccgta gcggggcagc tgaagcggtc
     2341 gcgcccggtg tcagcgcgtc cacaaatcga cacagaaaaa ttcaaatttc cttttgacat
     2401 caaagttcag atctgtagaa tgcgcgtctc gcccaagggg gtggttagct cagctggtag
     2461 agcacctgcc ttacaagcag ggggtcagcg gttcgatccc gttaccaccc accacgcggc
     2521 gagcgagttc caggagcggt agttcagtcg gttagaatac cggcctgtca cgccgggggt
     2581 cgcgggttcg agtcccgtcc gctccgccat tactcacgtc caagccgctg tcttcagcgg
     2641 ctttttcgtt tctggtgtca gcatacttcc caatttgcgt gccgacttta gtcggagacg
     2701 ggaagtacag actggcaata cccggtatgc gccctccgac gtctacactc gtcccaatct
     2761 acttgcgggg tgaggggggc gccggggccg tggtcatcgc gccgcggctg gtgaacgacg
     2821 cagacgcggc gcggcacaac ttccggcggg gcgtggccga acgcgccgtg gtcagctgag
     2881 cggcggtctc aggaggcccc gtccgcccag gccagcaagc gcgcgagaaa acgctcgacg
     2941 ccgcgatcgc aggcgcgctt ggccggatag aacacgaccc ccatgacgcc ggccgcgacc
     3001 agcgggggcg tttccccggg cagaccccgg cgcaggaaat cgcccagcag ggtctcgatc
     3061 gcgcccagca cgaacaggct cagcagcagc atggacccgt acacggccgt gcgccggaac
     3121 agccgcgcgg cgtcgatcgg gccgcggtgc agcagtcccg ccacgaacga cagcagcagg
     3181 atcggcagcg gcgcgaccag caaccagtcc tggtatcggc cgagccagca gagcgccgac
     3241 tcgccggccg gcgtgcaccc ggcgagacgc ccgccccaga ccagcaactg ccaggccgcg
     3301 gccagcgttg ccgccaggac ccagccgctc aggaagcaca gcgcctggcg ccggccgtcg
     3361 gcgtcgagcg cgcgcaggtt gcgcgctatc gtggtcacga gcacgatcat gcagaccgcc
     3421 agccacaggt tcatcaccag cagcgcgccg ggcacggctt cgatgcgcag ggccgtggcc
     3481 agcgcgagca ggcccggtaa ggtgaacagc aggcctggcc gcgcctcggc aacgcgccag
     3541 gccgccgcca gcagcaacag cgcggcgagt gcgagctcga tgggcagggc atcgacggcc
     3601 cgggccaggc cgctcccctc gtacagcgtg aacagcgtgg ccgacgccag cagccacgac
     3661 gagccggcgc gtgccggtgg cggcgcgcgt cgccacagga gcaggcccgc cacggcgagg
     3721 gacagcaccg tcgcggtgat cgcggccgtg cgcgggagcg tcaacgcgac gcacgcgaac
     3781 gacagctgga tgagcgccgc ttgcgctgcg atgtggggcc ggggcgacgc cgcgataccg
     3841 agcacgctgc ggacgaagtg gatcgccacg gcgcccgcga aggtccatcc ggcgatccac
     3901 agcagccgct ccagcgccgc caccgcgtgg ccggtgccgc gcagcagcgc gacgtccacc
     3961 ccgttgcgct gcaacagcac cacggcctgc aggtagccgt cgaagagcgg gctgctcacc
     4021 gccgtgacca tcacgccgta gccgcccgcg agcagcgcgc agaagagcgc gagccagcgc
     4081 gcgggcg
//
LOCUS       DAORUQ010000245         4073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_28373_length_4073_cov_6.021653, whole genome shotgun sequence.
ACCESSION   DAORUQ010000245 DAORUQ010000000
VERSION     DAORUQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28373_length_4073_cov_6.021653"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2504
                     /locus_tag="PLW13_14850"
     CDS             156..2504
                     /locus_tag="PLW13_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY71707.1"
                     /translation="MPRAALTVCRQLCAALLLLLPSLQGLAREPVAPARVTWLVVVAV
                     CVIGLIVATLYWWDRRPRVAAAALVLLALGAAGLFEWRFREQRANEQRLELLERLGSL
                     RSRLESVIAKNLALTNGLAAFVAANPDLGQEEFDAYARNVLAREPSLRNLAAAPDLVV
                     RYVYPREGNEAVLGLDYRADPAQREVAERVMRSGDLVVAGPVELVQGGTAFIGRAPVY
                     IETPGQPRVTWGLVSAPIDVHKVLQQGGLAAPMPEGLRIAIRGKDGKGERGEVFHGDA
                     SVFDARDIVTTPVLIGGGSWQIGAVPGTDPRTVYGVRVIRLCALVLLALLLLALQATA
                     RARARERDYALALERQANFDPLTGLPNRPLFRQQLESAIARSGRTGARHALLFIDLDE
                     FKSVNDNLGHDAGDQLLIDVGRRICGCVRTSDTVARLSGDEFTAILYDIVAEESISRV
                     AEAIVEALGRAFRIGEREVFCGASVGVAIYPDDASSADSLMIKADQAMYAVKRSGRNG
                     WYFYTREMQEKSERRHRLYNELVGALDDGRIVAHLQPIVSVQTGRIVSCEALARWQRE
                     DGSWVSPGEFVPVAEESGLINRVDFVVMQTAIDAVRAINEQLGTGIGLSANVSPRIFA
                     AQNQQIQRWMDTVKAAARELRLTVEITERLLFDDAAEAHKALNELAASGVGISIDDFG
                     TGNASLIHLTRFPVGIIKIDRSFTRAIGLGRAEEALIETILVMAKRLDLRVVAEGVET
                     REQYDYLSARGCDFVQGHFLGRPVDAAQFLEMARAAGTPRGG"
     gene            2528..3904
                     /locus_tag="PLW13_14855"
     CDS             2528..3904
                     /locus_tag="PLW13_14855"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQY71708.1"
                     /translation="MNERYRVEHDSLGAIEVPADALWGAQTERLRRNFPIGTQRFAPH
                     FIAAFALVKKACALANAELGDLDAARAALIAAACDEIIAGRLDAEFPLSVWQTGSGTQ
                     TNMNLNEVIANRAHQLGGSAGPALHPNDHVNRAQSSNDVFPAVMHVAAVQRCDERLLP
                     ALHTLRRALAGRAGAFADLLKTGRTHLMDAAPVTLGQEFGAYAAQLGFCIATIAEARE
                     SLLALALGATAVGTGLNAPPGWPARVCARLAQLGGVPFRPADNLFMAIAAHEPMLKFS
                     AALRLLATALLKIANDIRLLASGPRCGLAELRLPANEPGSSIMPGKVNPTQAEALTMV
                     AVQVLGNDAAVAIAASQGVLELNVYKPLIIHNVLESVELLADACESFALRCVDGIEAD
                     PARLAAYVEASLMLATALNPHIGYEAAARIARHAHEHGLSLRAAALALGLVSAEDFDR
                     WVRPADMV"
BASE COUNT          578 a         1409 c         1478 g          608 t
ORIGIN      
        1 gtggctgatg aatgcgggtc gcggcaattg tcccgtacct taaagcattt ggggatgccg
       61 gcgtagcgtc gccgatgtgc gccattgcgc tgtctggcgg cgtcgcggcc cgctactatg
      121 cgtgttccga cccatcgacc cgtgcgccga ccccgatgcc cagagccgcc ctgaccgtgt
      181 gtcgccagct gtgcgcggcc ttgctgctgt tgctgccatc cctgcagggg ctcgcgcgcg
      241 agcccgtggc gccggcgcgc gtgacgtggc tggtcgtggt cgccgtgtgc gtgatcggcc
      301 tcatcgtggc gaccctgtac tggtgggatc gccgcccgcg cgttgccgcc gccgcgctgg
      361 tgctgctcgc cctcggcgcc gcggggctgt tcgagtggcg attccgcgag cagcgcgcga
      421 acgagcagcg actggaactg ctcgagcgcc tcggctcgct gcgcagccgc ctcgagagcg
      481 tgatcgcgaa gaacctcgcg ctgaccaacg ggctggcggc attcgtcgcg gccaaccccg
      541 acctcggcca ggaggagttc gacgcctatg cgcgcaacgt cctcgcgcgc gagccgtcgc
      601 tgcgcaacct ggccgccgcg cccgacctgg tcgtgcgcta cgtctatccg cgcgagggca
      661 acgaggcggt gctcgggctc gattaccggg ccgatccggc gcagcgcgag gttgccgagc
      721 gcgtgatgcg cagcggcgac ctggtggtgg cggggccggt ggaactggtg cagggcggca
      781 cggcattcat cgggcgcgcg ccggtctaca tcgagacgcc gggccagccg cgcgtgacct
      841 gggggctggt gtcggcgccg atcgacgtgc acaaggtgct gcagcagggc ggtctcgcgg
      901 cgccaatgcc cgagggcctg cggatcgcga tccgcggcaa ggacggcaag ggcgagcgcg
      961 gcgaggtgtt ccatggcgat gccagcgtgt tcgatgcccg ggatatcgtc accacgccgg
     1021 tgctgatcgg cggcggttcg tggcagatcg gggccgtgcc cggaacggac ccgcgcaccg
     1081 tctacggcgt gcgggtgatc cgcctctgcg cgctcgtgct gctcgcactg ctgctgctcg
     1141 cgttgcaggc caccgcgcgg gcgcgcgcca gggagcgcga ttacgccctg gcactggagc
     1201 gccaggccaa cttcgatccg ctcaccggcc tgccgaaccg cccgctgttc cgccagcagc
     1261 tcgagtcggc gatcgcgcgc tcgggccgca ccggtgcgcg ccacgcgctg ctgttcatcg
     1321 atctcgacga gttcaagtcg gtgaacgaca acctcgggca cgacgcgggc gaccagctgc
     1381 tgatcgatgt cgggcgccgc atctgcggct gcgtgcgcac ctccgacacc gtggcgcgcc
     1441 tgagcggcga cgagttcacc gccatcctct acgacatcgt ggccgaggaa tcgatctcgc
     1501 gcgtggccga ggccatcgtc gaggcattgg ggcgggcgtt tcgcatcggc gagcgcgagg
     1561 tgttctgcgg cgcgagcgtc ggcgtggcga tctacccgga cgacgcgagc agcgccgact
     1621 cgctcatgat caaggccgac caggccatgt acgcggtgaa gcgctcgggg cgcaacgggt
     1681 ggtatttcta cacgcgcgag atgcaggaga aatccgagcg ccgccaccgc ctctacaacg
     1741 aactggtcgg cgcgctggac gacgggcgca tcgtggcgca cctgcagccg atcgtgagcg
     1801 tgcagaccgg gcggatcgtc agctgcgagg cgctggcgcg ctggcagcgc gaggacggat
     1861 cctgggtgtc gccgggcgaa ttcgtgccgg tcgccgagga gtcggggctg atcaaccggg
     1921 tggacttcgt cgtgatgcag accgcgatcg acgcggtgcg cgcgatcaac gagcagctcg
     1981 gcaccggcat cggcctgtcg gccaacgtct cgccgcgcat cttcgcggcg cagaaccagc
     2041 agatccagcg ctggatggac acggtcaagg cggccgcacg cgagctcagg ctcacggtgg
     2101 aaatcaccga gcggctactg ttcgacgatg ccgccgaggc gcacaaggcg ctcaacgagc
     2161 tggcggccag cggcgtcggc atctcgatcg acgatttcgg caccggcaac gcctcgctga
     2221 tacacctgac gcgcttcccg gtggggatca tcaagatcga ccgctcgttc acgcgcgcga
     2281 tcggcctggg tcgtgccgag gaggcgctga tcgagaccat cctcgtgatg gcgaagcggc
     2341 tggatctgcg cgtcgtggcc gaaggcgtcg agacccggga gcagtacgat tacctgagcg
     2401 cccgcggctg cgacttcgtg cagggccatt tcctcggccg tccggtggac gcggcgcaat
     2461 tcctggaaat ggcccgcgcc gccggtactc cgcgcggcgg ctgagcgatg cggggaggag
     2521 cgattccatg aacgagcgtt atcgcgtgga gcacgacagc ctgggcgcga tcgaggtgcc
     2581 cgccgacgcg ctatggggcg cgcagaccga gcgcttgcgg cgcaatttcc cgatcggcac
     2641 gcaacgcttc gcgccgcatt tcatcgccgc cttcgcgctg gtgaagaagg cctgcgcgct
     2701 ggcgaacgcg gagctcggcg acctcgacgc cgcgcgcgcg gcgctcattg ccgcggcctg
     2761 cgacgagatc atcgcggggc gcctggacgc cgagtttccg ctgagcgtgt ggcagaccgg
     2821 cagcggcacg cagaccaaca tgaacctcaa cgaggtgatc gccaaccgcg cgcaccagct
     2881 cggcggcagc gcgggccccg cgctgcatcc caacgatcac gtgaaccgcg cgcagtcctc
     2941 caacgacgtc tttcccgcgg tgatgcacgt cgccgcggtg cagcgctgcg acgagcgcct
     3001 gctgccggcg ctgcacacgc tgcggcgcgc gctcgccggg cgcgccgggg cgttcgcgga
     3061 cctgctgaag acggggcgca cgcacctgat ggatgccgcg ccggtgacgc tgggccagga
     3121 gttcggcgcc tacgccgcgc agctcgggtt ctgcatcgcg acgatcgccg aggcgcgcga
     3181 atcgctgctc gcgctcgcgc tgggcgcgac ggcggtgggc accgggctca atgcgccgcc
     3241 cggctggccg gcgcgcgtat gcgcgcggct ggcgcagctc ggcggcgtgc cgtttcgccc
     3301 ggcggacaac ctgttcatgg cgatcgccgc gcacgagccg atgctgaagt tcagcgccgc
     3361 gctgcgactg ctcgccacgg cgctgctgaa gatcgccaac gacatccgcc tgctcgcgag
     3421 cgggccgcgc tgcggcctgg ccgagctgcg cctgccggcc aacgagccgg gcagctcgat
     3481 catgcccggc aaggtgaatc cgacgcaggc cgaggcgctc acgatggtgg cggtgcaggt
     3541 gctaggcaac gacgcggcgg tcgccatcgc cgccagccag ggcgtgctcg agctgaacgt
     3601 ctacaaaccg ctgatcatcc acaacgtgct cgagtcggtc gagctgctcg ccgatgcctg
     3661 cgagagcttc gcgctgcgtt gcgtggacgg catcgaggcc gatcccgcgc gcctcgcggc
     3721 ctatgtcgag gcgtcgctga tgctggcgac cgcgctgaac ccgcacatcg gctacgaggc
     3781 ggcggcacgc atcgcgcgcc acgcccacga gcacgggctg tcgctgcgcg cggcggcgct
     3841 ggcgctgggg ctggtcagcg ccgaggactt cgaccgctgg gtgcgcccgg cggacatggt
     3901 ctgatatcgt tcgccagccc cgacacgcgc cgatgcgctc cgcgaggaaa ccatgcagct
     3961 gcaacttgcc ttcgaactgc gctcgcccga ctgcggcgcg ccgcatcgcg agctctacgc
     4021 cgcgatgctc gacatctgcg cctgggccga cgagctcggc ttcgacgtgg tca
//
LOCUS       DAORUQ010000246         4048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_28664_length_4048_cov_5.432256, whole genome shotgun sequence.
ACCESSION   DAORUQ010000246 DAORUQ010000000
VERSION     DAORUQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28664_length_4048_cov_5.432256"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="PLW13_14860"
     CDS             complement(<1..2023)
                     /locus_tag="PLW13_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71709.1"
                     /translation="MKISSAKQDKPRNRIVLGAAVAALLPGLAQAQRADGASGIEEIL
                     VTAQRREERLQDVPIAISAISAEQIERRRIDNVLDLKALAPNLMVSRYPNSNVVSQVA
                     IRGGVTVNAAMYWEPSTGMYLDGVYLGKAVGSVFDVVDIERIEVLRGPQGTLYGRNTM
                     AGAVNLITRKPSGEFGGSATVELGNYDHHVEKFSVDLPKVGIARMSFGVRSESRDGLV
                     DLESGSPGTELDSRDNFGARFALGLDISDCVVADYRFDYTDVDQMPPHSQLYSVTPSN
                     ALFTAAAAYASRDREDSAATNWPGYEQLELKGHALTLGWDINETNTLKSITSYRDLRN
                     DDSVDLDGVPLTIATANRISDYDQTSQELQWIGSTSRLHYVLGLYYYEDDGYTINPHV
                     FFFGTDSSQYGFGAEAMAAYAQFDFRATDALTLTAGLRYTDEDKDTERYKTVTGAGAP
                     IARVEADASFSDTTPMASAAYRFSDQLNVYLKYAEGFKSGGFMGEAGNAVEAVTPYDP
                     EQQSTWEVGAKMSTEDGRLQLNTAAFHNEIDDMHVNQFTGLPGVSVVRNAGQATTQGV
                     EIEGIWLPTDALRLQLSYGYLDAEWDEFMEAPAIGQPITNVADNRSFPHAPEHTLNLM
                     ADARLAQTAWGELRALADYSYTASFYAYAYQLETIDTRRATAGNTEVDAY"
     gene            2346..3596
                     /locus_tag="PLW13_14865"
     CDS             2346..3596
                     /locus_tag="PLW13_14865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQY71710.1"
                     /translation="MNSSNMPLQGLRIIESSLLGAAAITTSLADLGATVIKVESPEGD
                     YIREMTWPIIEGVSLMHYHLNRGKKSITLDLKTEAGKELYRELVRDADVVIEAMRPGV
                     LAKLGLGFEDLRKVNPAIVFCTVSGYGMTGPYKDMPSHGIAYDTWSGAVTPAYDEEGF
                     CYIPEHVSIGINAAPLFGGLAVLAGVLRARATGAGCMMELGQSDAAAFFDWYRCESHL
                     AYARPQDVVTGNKADGYVRRPVATAGMKEGVRYQIYASSDGHVLFMASEQAFWKNFCE
                     GVGRGDLFEKWPGSRYADHARGNRELQRELREIFRSRTSAQWMEFSARANTPIAPVNT
                     PQNIVDDPQFKARFDLLPHETHGADMLSFPVHFVGEQLLPPARAPVAGEHTEQVLREV
                     LGCDDARVAAIRGSGALGAAAAKD"
     gene            3723..>4048
                     /locus_tag="PLW13_14870"
     CDS             3723..>4048
                     /locus_tag="PLW13_14870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71711.1"
                     /translation="MEFEFSAEEREFIRQVDQFLKENESRDVMDVSRENMAQVCDTPE
                     RRAFMKKIAERGWLGITWPREHGGQDGSGIYEYLLNEMLSAVGAPGIGKGVGIVGKTL
                     INVGSE"
BASE COUNT          672 a         1282 c         1361 g          733 t
ORIGIN      
        1 cgtaggcatc cacctccgtg ttacccgccg tggcgcggcg cgtatcgatg gtctccagct
       61 ggtaggcata cgcgtagaac gacgcggtgt agctgtagtc ggccagggcg cgcagttcgc
      121 cccacgccgt ctgcgccagc cgcgcatccg ccatcaggtt gagggtgtgc tccggcgcgt
      181 gggggaagga gcggttgtcg gccacgttgg tgatcggctg cccgatcgcg ggcgcttcca
      241 tgaactcgtc ccactcggcg tcgaggtagc cgtagctcag ctgcaggcgc agcgcatccg
      301 tcggcagcca gatgccctcg atctccacgc cctgcgtggt cgcctggccg gcattgcgca
      361 cgaccgatac accgggaagg ccggtgaact ggttcacgtg catatcgtct atctcgttgt
      421 ggaacgcggc ggtgttcagt tgcagccggc catcttcggt gctcatcttg gcgccgacct
      481 cccaggtgga ctgctgctcg gggtcgtagg gtgtcaccgc ctccacggca ttgccggcct
      541 cgcccatgaa gccgccgctc ttgaagccct cggcgtactt gaggtagacg ttcagttggt
      601 cgctgaacct gtaggccgcg cttgccatcg gcgtggtgtc ggaaaacgac gcgtcggcct
      661 cgacccgtgc tatgggagcc ccggccccgg tgacggtctt gtagcgctcg gtgtccttgt
      721 cttcatccgt gtagcgcagt cccgccgtga gcgtcaacgc gtccgtggcg cggaaatcga
      781 actgcgcgta ggcggccatc gcctcggcgc cgaagccgta ctgcgagctg tccgtgccga
      841 aaaagaagac gtgcgggttg atcgtgtagc cgtcgtcctc gtaatagtac aggccgagga
      901 cgtagtgcag gcgtgatgtg ctgccgatcc actgcagttc ctgcgaggtc tggtcgtagt
      961 ccgagatgcg attggccgtg gcgatggtga gcggcacgcc gtcgaggtcc accgaatcgt
     1021 cattgcgcag atcgcgatac gacgtgatcg acttcagggt gttggtttcg ttgatgtccc
     1081 agccgagcgt cagcgcgtgg cctttgagct ccagctgctc gtagccgggc cagttggtcg
     1141 ccgccgagtc ttcccggtcc ctggatgcat acgccgcggc cgcggtgaac agcgcgttgg
     1201 acggcgtcac gctgtagagt tggctgtggg gcggcatctg gtcgacatcc gtatagtcaa
     1261 accggtaatc cgcgaccacg cagtcgctga tgtccagccc gagcgcgaaa cgcgcgccga
     1321 aattgtcacg gctgtcgagc tcggtgcccg gactgccgga ctcgagatcc accagcccgt
     1381 cgcggctctc cgagcgcacg ccgaaggaca tgcgcgcgat gcccaccttg ggcaaatcca
     1441 ccgagaattt ttccacgtga tggtcgtagt tgcccagctc gaccgtcgcg gagccgccga
     1501 attcgccgct gggcttgcgc gtgatcaggt tgaccgcgcc cgccatggtg ttgcgcccgt
     1561 acagggttcc ctgtggcccg cgcagtacct cgatgcgctc gatatccacc acgtcgaaga
     1621 ccgagcccac tgccttgccg agatatacgc cgtcgaggta catccccgtg ctcggctccc
     1681 agtacatcgc ggcgttcacc gtgacgccgc cgcgaatcgc cacctgagaa acaacgttgc
     1741 tgttcggata cctgctcacc atgaggttcg gcgccagcgc cttgaggtcg agcacgttgt
     1801 cgatccggcg cctctcgatc tgttccgcgg agatggcgct gatggcgatg ggcacgtcct
     1861 gcagacgctc ttcgcgcctc tgcgcggtaa ccaggatctc ctcgattccg gaagcgccat
     1921 cggcccgctg ggcttgtgcg aggcccggga gcagcgcggc aacggcggcg ccgaggacga
     1981 tgcggttgcg gggcttgtct tgctttgcgg agctgatttt catgatgtat ctcccgtggt
     2041 tccgatggca agtccggcga tcgacgccat cgtgtatcga tttattcccc tgtcggtggg
     2101 taccaacggt gcgcaaacac ccctgtcgga cgccccgacg cagggagaat agtcgcgcga
     2161 ggcgccgcgt cgagccgcag tgcgcctgtt gtcccgaact gataccactt tggccacaac
     2221 cgagttgccg cagggctgag cccgtctgcg gtcttgaata tcttggtcgt cgatgcgcga
     2281 cgaacgcagc ggctgtgccg cggcgccact cgtgcctttc acagcacatg ccgggagaac
     2341 gcgatatgaa ttcctcgaac atgccactgc aggggctgcg gatcatcgag agctccctgc
     2401 tcggggccgc ggcgatcacg acatcgctcg cggacctcgg cgcgaccgtg atcaaggtcg
     2461 aatcgccgga gggcgactac atccgcgaga tgacctggcc catcatcgag ggtgtctcgc
     2521 tgatgcacta ccacctgaac cggggcaaga agagcatcac gctcgacctg aagaccgagg
     2581 ccggcaagga gctctatcgc gaactggtgc gcgatgccga tgtggtgatc gaggccatgc
     2641 gccccggggt gctcgccaag ctgggcctgg ggttcgagga cctgcgcaag gtcaatccgg
     2701 cgatcgtctt ctgcacggtg tcgggatacg ggatgacggg tccctacaag gacatgccct
     2761 cgcacgggat cgcctacgac acctggtcgg gcgcggtcac gcccgcctac gacgaggagg
     2821 gcttctgtta catacccgag cacgtctcga tcggcatcaa cgcggcgcct ctgttcggcg
     2881 ggctcgcggt gctggccggc gtgttgcgcg cacgcgccac cggcgccggc tgcatgatgg
     2941 agctggggca gagcgatgcg gcggcgttct tcgactggta tcgctgtgag agtcacctgg
     3001 cctacgcacg tccgcaggac gtggtcaccg gcaacaaggc cgacggctac gtgcggcggc
     3061 ccgtggcgac ggccggcatg aaggagggcg tgcgctacca gatatacgcc tccagcgacg
     3121 gccacgtgct gttcatggcc tcggagcagg ccttctggaa gaacttctgc gagggggtcg
     3181 ggcgcggcga cctgttcgag aagtggccgg gatcacgcta tgccgaccac gcgcgcggca
     3241 accgcgaact gcagcgggaa ctgcgcgaga tattccgttc gcgcacctcg gcgcagtgga
     3301 tggagttcag cgcccgcgcc aacacgccga tcgcgccggt caacacgccg cagaacatcg
     3361 tcgacgaccc gcagttcaag gcgcgcttcg atctgctgcc gcacgagacg cacggtgccg
     3421 acatgctgtc gtttccggtg cacttcgtgg gcgagcagct gctgccgccg gcacgcgcgc
     3481 cggtggccgg cgaacacacc gagcaggtgc tgcgcgaggt gctgggctgc gacgacgccc
     3541 gcgtggcggc gatccggggt tccggcgcgc tgggcgccgc cgcggccaag gactagggga
     3601 atgcaccggt gtccttttgt gataccgatt tggcctgtta aggcgtgctg ccacgctcga
     3661 agcgggcgcg cacatcgcta gaatccgaac aacgcactgg gttttgcagg ggacagacgg
     3721 acatggaatt cgaattttcc gccgaggaac gtgaattcat ccgccaggtc gaccagttcc
     3781 tcaaagagaa cgagtcgcgc gacgtgatgg atgtgagtcg cgagaacatg gcgcaggtct
     3841 gcgacacgcc cgaacggcgc gcattcatga agaagatcgc cgagcgcggg tggctgggca
     3901 tcacgtggcc cagggagcat ggcgggcagg acggcagcgg catctacgaa tacctgctca
     3961 acgagatgct ctccgccgtc ggcgctcccg gcatcggcaa gggtgtcggc atcgtcggca
     4021 agacgctgat caacgtggga tcggaaaa
//
LOCUS       DAORUQ010000247         4019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_28967_length_4019_cov_6.468214, whole genome shotgun sequence.
ACCESSION   DAORUQ010000247 DAORUQ010000000
VERSION     DAORUQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28967_length_4019_cov_6.468214"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3986
                     /locus_tag="PLW13_14875"
     CDS             <1..3986
                     /locus_tag="PLW13_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY71712.1"
                     /translation="VPMRTLSATMRRAQEGETDARAALEGTREIVHMQQAFNTMMEVL
                     RSREEAIADARDQALAQERVAQEARDEALEQKSLAQEARDQALEQGKALKIARDQALE
                     SARAKGEFAATVSHELRTPLNGVIGMLDLIADMDLSNKQLDYVQTARSSADMLLNLIN
                     DILAFSKIDAGKTTLEVVEFELRDKLEEVLALVTPQAQNKDLELAYVIDGNIPPSVLG
                     DVHHLGQVLLNLLGNAIKFTETGSIGAVVTLVEEDGVHLRLRFEVSDTGVGIDPAVQK
                     QIFEAFSQADSSTTRRYGGTGLGLAICRELVRLMGGEISVDSMPGKGSRFHFDLPFIR
                     SRQGSSQARALLPLTREAGVLLCDDNPLVQRSMASMLAGERVQLTTVDSIAGATAALS
                     GAPARFDVAIIDERMVDPAAQPFIDALGRGAQNVVRLGVRPRADGYWLPRLPIAQALG
                     KPLRRSTVLDCLRALLANQAPAPRTVPRAEGARTRDTFRNSRVLVVEDNRANQEVAIG
                     MLQRLGCEIAIANDGIEALERLEQQEYELVLMDCHMPRMDGFEATRRIREMNAPFSNV
                     TIVAMTANVQKSAMDHCHAVGMNDYLSKPIKLGVLRDMLNRWLAADEESALVDDGATA
                     RAGAANVVIDKAYLAELREQIGPAVDRVTTAFLEDLPGYIAQLREASAAQEMPEIVRL
                     AHTLKGAATNTGANRLAHKAQQLEQEARSGTRSACENLVSALVYESNLVRHVLEGDTA
                     RGDDSAPVPLERRRQQERLFRILVVDDDRGARFALRETLLGEGYEVVDAGNGQQAVQI
                     CRQQAPDLVILDGVMPVMDGFQSCEVIRSLPECATIPILIVTGLNDDASVDKAFSVGA
                     TDYISKPINYAVLRRRIDHMLQVHSAEKRVHQLAYIDSLTGRPNRTKLNEHMDALFSD
                     TSRKDKQFALLFLDLDNFKLVNDTLGHEAGDLLLKYVSERLTGCVRKGDLVARFGGDE
                     FCIVLDGVRSYEMVRNIAEKIHDHLKRPFVFLGREMLLSMSIGIAVYPEHGQDAVSLL
                     KAADMAMYDAKKSQKGFGFYESHLESDNLERLELENDLRNAIARDELLLYYQPQEDLS
                     TGQIFGLEALIRWQHPRRGLVSPAEFIHLAEETGLINDIGLWVLCTICEQINAWMDRG
                     FAAPRVAINLSARQLANPNLATTFREVISDSRLPADLIEFEITESTIMERPDEMIDML
                     KELKAMGTRLAVDDFGTGYSSLSYLKRFPLDYIKIDRAFISDVMSNRIDADIVRTIIA
                     LAQALEVKVIAEGVETAQQRQFLKEHNCHYAQGYLLSKPVPAAEIERRFLKRADASNL
                     VPFPKSS"
BASE COUNT          727 a         1399 c         1323 g          570 t
ORIGIN      
        1 ccgtgccgat gcgcaccctc tccgcgacga tgcgccgcgc gcaggaaggc gaaactgacg
       61 cgcgcgccgc gctggagggc acgcgcgaga tcgtgcacat gcagcaggca ttcaacacca
      121 tgatggaagt gctgcgcagc cgcgaggagg cgatcgccga cgcgcgcgac caggcgctgg
      181 cccaggaacg cgtcgcgcag gaagcccgcg acgaggccct cgagcagaag agcctcgcgc
      241 aggaggcccg cgaccaggcg ctggaacagg gcaaggcgct gaagatcgcg cgcgaccagg
      301 cgctggagtc ggcgcgcgcc aagggcgagt tcgccgccac cgtgagccac gagctgcgca
      361 cgccgctgaa cggtgtgatc ggcatgctcg acctgatcgc cgacatggac ctcagcaaca
      421 agcagctcga ctacgtgcag accgcgcgca gctcggccga catgctgctg aacctgatca
      481 atgacatcct cgccttctcc aagatcgacg ccggcaagac cacgctggaa gtcgtcgagt
      541 tcgagctgcg cgacaagctc gaggaagtcc tcgcgctggt cacgccgcag gcacagaaca
      601 aggacctcga gctcgcctat gtcatcgacg gcaacatccc gccgagcgtg ctgggcgacg
      661 tgcaccacct cgggcaggtg ctgctgaacc tgctcggcaa cgcgatcaag ttcaccgaga
      721 ccggatcgat cggcgccgtg gtgacgctgg tcgaggaaga cggcgtgcac ctcaggctgc
      781 gcttcgaggt cagcgacacc ggcgtgggca tagaccccgc ggtgcagaag cagatcttcg
      841 aggctttctc gcaggccgac agttccacca cgcgccgtta cggtggtacc ggcctggggc
      901 tggcgatctg ccgcgaactg gtgcgcctga tgggtggcga gatctccgtc gacagcatgc
      961 ccggcaaggg cagccgcttc catttcgatc tgcccttcat ccgttcacgc cagggcagct
     1021 cgcaggcgcg cgcgctgctg ccgctcacgc gcgaggccgg cgtgctgctc tgcgacgaca
     1081 acccgctggt gcagcgctcg atggccagca tgctggcggg cgagcgcgtg cagctcacga
     1141 cggtggactc catagcgggg gcaaccgcgg ctctttcggg cgcgcccgcg cgcttcgacg
     1201 tcgcgatcat cgacgagcgc atggtcgacc ctgcagccca gccgttcatc gacgccctcg
     1261 gacgcggggc gcagaacgtg gtgcgcctgg gcgtgcgtcc gcgcgccgac ggctactggc
     1321 tgccgcggct gccgatcgcg caggcgctcg gcaagccgct gcgccgctcc acggtactcg
     1381 actgcctgcg cgccctgctc gccaaccagg cgccggcacc acgcaccgta ccgcgcgccg
     1441 agggtgcccg gacgcgcgac accttccgca acagtcgcgt gctcgtggtc gaggacaacc
     1501 gcgcgaacca ggaagtggcc atcggcatgc tgcagcgcct gggctgcgag atcgcgatcg
     1561 ccaacgacgg catcgaggcg ctcgagcgcc tcgagcagca ggaatacgaa ctggtgctga
     1621 tggactgcca catgccgcgc atggacggct tcgaggccac gcggcgcatc cgcgagatga
     1681 acgcgccgtt cagcaacgtg accatcgtgg cgatgaccgc caacgtgcag aagagcgcga
     1741 tggaccattg ccacgccgtg ggcatgaacg actacctgtc caagccgatc aagctcggcg
     1801 tgctgcgcga catgctgaac cgctggctcg ccgccgacga ggagtcggcg ctcgtcgacg
     1861 acggcgccac ggcccgcgcc ggcgccgcga acgtggtgat cgacaaggcc tatctcgcgg
     1921 agcttcgcga gcagatcggc cccgcggtgg accgcgtcac cacggccttc ctggaggacc
     1981 tgcccggcta catcgcgcag ctgcgcgagg ccagcgccgc acaggagatg ccggagatcg
     2041 tgcgcctcgc gcacacgctg aagggcgcgg cgaccaacac cggcgcgaac cgcctggcgc
     2101 acaaggcgca gcagctcgag caggaggcgc gcagcggcac gcgcagcgcc tgcgagaacc
     2161 tggtctcggc actggtctac gaatcgaacc tggtgcgcca cgtgctcgag ggcgacaccg
     2221 cccgcggtga cgacagcgcg cccgtgcccc tcgagcggcg ccggcagcag gagcgcctgt
     2281 tccgcatcct ggtggtagat gacgaccgcg gcgcgcgatt cgcgctgcgc gagaccctgc
     2341 tcggcgaagg ctacgaggtc gtcgatgcgg gcaacggcca gcaggcggtg cagatctgcc
     2401 gccagcaggc cccggacctg gtgatcctcg acggcgtgat gccggtgatg gacggtttcc
     2461 agtcctgcga ggtcatccgc agcctgcccg aatgcgccac gatcccgatc ctgatcgtca
     2521 ccggcctgaa cgacgacgcc tcggtggaca aggcgttcag cgtcggcgcc accgactaca
     2581 tcagcaagcc gatcaactac gccgtgctgc gccggcgcat cgaccacatg ctgcaggtgc
     2641 acagcgcgga gaagcgcgtg caccagctcg cctacatcga ctccctcacc ggtcgcccga
     2701 accgcaccaa gctcaacgag cacatggacg cgctgttctc cgacacctcg cgcaaggaca
     2761 agcagttcgc gctgctcttc ctcgatctcg acaacttcaa gctggtgaac gacacgctgg
     2821 gccacgaggc cggcgacctg ctgctgaagt acgtctccga gcgcctcacc ggctgcgtgc
     2881 gcaagggcga cctggtcgcg cgcttcggcg gcgacgaatt ctgcatcgtg ctcgacggcg
     2941 tgcgctccta cgagatggtg cgcaacatcg ccgagaagat ccacgaccac ctgaagcggc
     3001 ccttcgtgtt cctcgggcgc gagatgctgc tcagcatgag tatcggcatc gcggtgtatc
     3061 ccgagcacgg ccaggacgcg gtgtcgctgc tgaaggcggc cgacatggcg atgtacgacg
     3121 ccaagaaatc gcagaaggga ttcggcttct acgagtcgca cctggagtcg gacaacctcg
     3181 agcggctgga actggagaac gacctgcgca acgccatcgc gcgcgacgag ctgctgctct
     3241 attaccagcc gcaggaggat ctctcgaccg ggcagatctt cgggctggaa gcgctgatcc
     3301 gctggcagca tccgcggcgc gggctggtga gcccggcgga gttcatccat ctcgccgagg
     3361 agacggggct gatcaacgac atcggcctct gggtgctgtg cacgatatgc gagcagatca
     3421 acgcctggat ggaccgcggc ttcgccgcac cgcgggtggc gatcaacctc tcggcgcgcc
     3481 agctcgccaa cccgaacctg gccacgacct tccgcgaggt catcagcgat tcgcgcctgc
     3541 ccgccgacct gatcgagttc gagatcaccg agagcaccat catggagcgc ccggacgaga
     3601 tgatcgacat gctgaaggag ctcaaggcga tgggcacgcg ccttgccgtc gatgacttcg
     3661 gcaccggcta ctcctcgctc agttacctca agcgcttccc gctcgactac atcaagatcg
     3721 atcgcgcctt catctcggac gtgatgagca accgcatcga tgccgacatc gtgcgcacga
     3781 tcatcgcgct ggcgcaggcg ctggaagtga aggtgatcgc cgagggcgtg gaaaccgcgc
     3841 agcagcgcca gttcctcaag gaacacaact gccactacgc gcagggctac ctgctgagca
     3901 agccggtgcc cgctgccgag atcgagcggc gcttcctcaa gcgcgccgac gcctccaacc
     3961 tggtgccctt ccccaagagc tcgtaggtcc gcacgtcacg cccctgtcgg ccccatcac
//
LOCUS       DAORUQ010000248         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_29547_length_3964_cov_6.675876, whole genome shotgun sequence.
ACCESSION   DAORUQ010000248 DAORUQ010000000
VERSION     DAORUQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29547_length_3964_cov_6.675876"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1085
                     /locus_tag="PLW13_14880"
     CDS             105..1085
                     /locus_tag="PLW13_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71713.1"
                     /translation="MRRAIALALCLGLAACGSRDAPLASLEVAEPGAFSAALSPDGRQ
                     AVIGSLRHGGSLWELDTDQRRFNWNHRAGESTPITAVAFAPGGDAVMTASHDTLVLWD
                     TASGGAITYFTAPAKVNAMALGPGGRLALLGLSDDTAVLFDARKGGIRRVFAHEGRVR
                     SVALDADARLALSGSEDRSARLWDVASGAQLQRWQHDADVRLVALAPDGARALSVSKY
                     DRAVVWDTSSGRELGALPAFRARVTRGETWTAAAFSADGARLLTGTTDRRVQLWDLPA
                     LERRAQWEMPRRAEWKRSGAYILAVGFGAAEGACLSVSADGFVHRLRLTP"
     gene            complement(1075..1698)
                     /locus_tag="PLW13_14885"
     CDS             complement(1075..1698)
                     /locus_tag="PLW13_14885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQY71714.1"
                     /translation="MGYSLGASMGRQFRNDKLQVDADALAKGVLEGFTADKLALTDEE
                     VAAGMQQLQKTHSERMQAEQKAAADKNLAEGEAFLKDNATKEGVVTTESGLQYRIVTA
                     GEGPKPGAEDVVKVHYRGTLIDGTEFDSSYKRGEPVTFPVGGVIPGWVEALQLMPVGS
                     KWELYIPSGLAYGPGGAGNQIGPNSALKFEVELLSIEPKEATEAATE"
     gene            complement(1925..2413)
                     /locus_tag="PLW13_14890"
     CDS             complement(1925..2413)
                     /locus_tag="PLW13_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HQY71715.1"
                     /translation="MGTEEFWEFALARYARPGVAQCCVALQDEHGANVMLLLYCAWRA
                     QGGDCVRGAALRQAIARLEPLDRHLVRPLRAARRAIRRAADALGSPALAVGADRLMQA
                     ELAAERRQARLLADGASSLRAGPARTCAAQSLAACLEGSGTAPAQAAIAGARLAALVF
                     DY"
     gene            2572..2901
                     /gene="trxA"
                     /locus_tag="PLW13_14895"
     CDS             2572..2901
                     /gene="trxA"
                     /locus_tag="PLW13_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HQY71716.1"
                     /translation="MSEHIVHVTDASFEEEVLKSKLPVLVDYWAEWCGPCKMIAPVLD
                     EIAREYAGRLKVCKMDVDANGETPARFAIRGIPTLMIFMNGNAEATKVGAVSRSQLAS
                     FIDSAIA"
     gene            3163..>3964
                     /gene="rho"
                     /locus_tag="PLW13_14900"
     CDS             3163..>3964
                     /gene="rho"
                     /locus_tag="PLW13_14900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQY71717.1"
                     /translation="MNLTELKEKPINELVEMAEAMGIENVARSRKQDVIFSLLKRHAK
                     SGEEISGDGVLEILSDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKDGERYFALLKVSDVNFSGSDTSRTKVLFENLTALFPQERLKLEQGNGSSQDLSGR
                     IMDLVSPIGKGQRGLIVSPPKAGKTLILQNIAQSIVRNNPECYVIVLLIDERPEEVTD
                     MERSVRAEVVASTFDEPPARHVQVADMVIEKAKRLVEHKKDVVILLDSI"
BASE COUNT          636 a         1375 c         1325 g          628 t
ORIGIN      
        1 gtgcaggacc tctcgacgct gggccaggtg ctggcatcgc gcttcgactg ggaagacgcg
       61 ctgatccgtc gcctgcacat ggccggacgc gcggtcgcct gatcttgcgc cgcgcgatcg
      121 cgctggccct ctgcctcggg ctggccgcct gcggatcgcg cgatgcgccg ctggcgagtc
      181 tcgaagtcgc cgagcctggc gcgttcagcg ccgcgctctc gcccgatggc cgccaggccg
      241 tgatcggctc gctgcgtcac ggcggcagcc tgtgggaact ggataccgat caacgccgct
      301 tcaactggaa tcaccgcgcc ggcgaaagca cgccgatcac ggccgtggcc ttcgcgcccg
      361 gaggcgatgc ggtgatgacg gcctcgcacg acacgctggt gctgtgggat acggccagcg
      421 gcggggcgat cacgtacttc accgcgccgg ccaaggtgaa cgcgatggcg ctcggacccg
      481 gtggacggct cgccctgctg ggactcagcg acgataccgc ggtgctgttc gatgcgcgca
      541 agggcggcat caggcgcgtg ttcgcccacg agggacgggt gcgcagcgtg gcgctggacg
      601 ccgatgcccg cctggcgctc agcggcagcg aggaccgcag cgcccgattg tgggatgtgg
      661 cgagtggcgc gcagttgcag cgctggcagc acgatgccga cgtgcgcctg gtcgcgctgg
      721 cgcctgacgg cgcgcgcgcg ctcagtgtca gcaaatacga ccgcgcggtg gtgtgggata
      781 catcatcggg gcgcgagctc ggcgcgctgc cggcgtttcg cgcgcgcgtc acgcgcggtg
      841 agacctggac cgccgcggcg ttctccgccg acggcgcacg gctgctcacc ggcacgaccg
      901 accggcgggt ccagttatgg gacctgcctg cgctcgagcg gcgggcgcag tgggagatgc
      961 cccgcagggc agagtggaaa cgctcaggcg cctacatact ggcggtaggc ttcggcgccg
     1021 cggagggcgc ctgcctgagc gtgtcggcgg acggattcgt gcaccgcctg cgccttactc
     1081 cgtagcggct tcggtcgctt ccttgggctc gatcgacagc agttccactt cgaacttcag
     1141 cgccgagttc ggtccgatct ggttgcccgc gccgccgggg ccgtaagcca ggcccgaggg
     1201 gatgtagagt tcccacttcg agccaaccgg catcagttgc agcgcctcga cccagcccgg
     1261 gatcacgccg ccgaccggga aggtcaccgg ctcgccgcgc ttgtaggagc tgtcgaactc
     1321 ggtgccgtcg atcagcgtgc cgcgatagtg caccttgacc acatcctcgg cgcccggctt
     1381 cggaccttcg ccggcggtga cgatccggta ctgcagaccg ctttccgtgg ttaccacgcc
     1441 ttccttggtg gcgttgtctt tcaggaaggc ctcgccctcg gcgaggttct tgtcggcagc
     1501 ggccttctgt tcggcctgca tgcgctcgga gtgcgtcttc tgcaactgtt gcatgccggc
     1561 ggccacttcc tcgtccgtca gggccagctt gtcggcggtg aaaccctcga gcacgccttt
     1621 ggccagcgcg tcggcatcga cctgcagctt gtcgttgcgg aactggcggc ccatcgaggc
     1681 accgaggctg taacccatgc gctgggcatc ggtgctgatc gcggcgccgc catcggcgtt
     1741 ggcggcatcc tcgggcgcct tggattcatt gcagcccgcg agcgcgagca gcgacacgca
     1801 gaatgccacg ggcagggaaa cggaacggtt catctggagt cctcgggggg gaatgacgcg
     1861 cgcatgcgcg ggagcgccgc atcatacatg aggccccgcg cgcttggata tgcgccgccc
     1921 gggctcagta atcgaacacc agcgcggcca gccgcgcgcc cgcgatcgct gcctgcgcag
     1981 gggccgtgcc gctgccctcg aggcatgcgg cgagcgattg cgcggcgcat gtgcgcgcgg
     2041 gccccgcgcg cagcgacgat gctccgtccg ccagcagcct ggcctggcgg cgctcggccg
     2101 cgagctccgc ctgcatcagg cgatcggcgc cgacggcaag cgcgggggag ccgagcgcat
     2161 cggcggcgcg acgaattgcg cgccgcgccg cgcgcagcgg acgcaccagg tgacggtcga
     2221 gcggttcgag gcgcgcgatc gcctggcgca acgccgcgcc ccgcacgcag tcgccgccct
     2281 gcgcgcgcca ggcgcagtac agcaacaaca tcacgttcgc gccgtgctcg tcctggagcg
     2341 ccacgcagca ctgcgccacg cccggacggg cgtagcgtgc gagcgcgaac tcccagaatt
     2401 cctccgtgcc catgatccct ccgtccatgc aaacggattc tcgccgaacg gcgcatgcgg
     2461 tgtcgcgctt gaaacgcgtc acggcaatcc ccatcttccc agcagatgat tcccgggtgc
     2521 tatgattcgc gcacagcccc gacaccaccc gcaacgcacc cggagaaacc gatgagcgag
     2581 catatcgtcc atgtcaccga cgcctccttc gaggaggaag tcctgaagtc caagcttccc
     2641 gtcctcgtgg actactgggc cgagtggtgc ggaccgtgca agatgatcgc gccggtgctc
     2701 gacgagatcg cgcgcgaata cgccggccgg ctgaaagtct gcaagatgga cgtggacgcc
     2761 aacggcgaga cgccggcgcg ctttgcgatc cgcggcatcc cgacgctgat gatcttcatg
     2821 aacggcaacg ccgaggcgac caaggtgggt gcggtatcgc gttcgcaact cgcctcgttc
     2881 atcgactcgg ccatcgcctg agtcccgatc gtgcgtgcgc gggccggtcg agggcccgcg
     2941 ccacgcgacg cccggggtgc tggactggcg ccgggaacgc gtgctacatt tgcccccgtt
     3001 caaatgcaac cagcccgtcc agtcactcct gatctgccgc tccaacccct gtttccgcgt
     3061 ctggatctgc gcttctcggc gtgactcgct ggcatctccg cgtgccgcct tccggtaacg
     3121 cacaaatcca ttcttcccat ccaacacacc acaccaacac acatgaacct gaccgaactc
     3181 aaagaaaagc ccatcaatga actcgtcgaa atggccgagg ccatgggcat cgagaacgtc
     3241 gcccgctcgc gcaagcagga cgtcatcttc tccctgctga aacgccacgc gaagagcggc
     3301 gaggaaatct ccggcgacgg cgtgctggaa atactctccg acggtttcgg cttcctgcgc
     3361 tcggcggatt cgagctacct ggccggcccc gacgacatct acgtctcgcc gagccagatc
     3421 cggcgcttca acctgcgcac gggcgacacc atctccggca agatccgccc gccgaaggac
     3481 ggcgagcgct acttcgcgct gctgaaggtc agcgacgtca acttctcggg ctccgacacc
     3541 tcgcgcacca aggtgctgtt cgagaacctc accgcgctgt tcccgcagga gcgcctgaag
     3601 ctcgagcagg gcaacggcag cagccaggat ctctcgggcc ggatcatgga cctggtctct
     3661 ccgatcggca aaggccagcg cggcctgatc gtctcgccgc cgaaggcggg caagacgctg
     3721 atcctgcaga acatcgcgca gtcgatcgtg cgcaacaatc ccgagtgcta cgtgatcgta
     3781 ttgctgatcg acgagcgccc ggaggaagtg accgacatgg agcgctcggt gcgcgccgag
     3841 gtggtcgcca gcaccttcga cgagccgccg gcgcgccacg tgcaggtggc cgatatggtg
     3901 atcgagaaag ccaagcgcct ggtcgagcac aagaaggacg tggtgatcct gctcgactcg
     3961 atca
//
LOCUS       DAORUQ010000249         3937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_29839_length_3937_cov_7.552808, whole genome shotgun sequence.
ACCESSION   DAORUQ010000249 DAORUQ010000000
VERSION     DAORUQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29839_length_3937_cov_7.552808"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /gene="tkt"
                     /locus_tag="PLW13_14905"
     CDS             complement(<1..1982)
                     /gene="tkt"
                     /locus_tag="PLW13_14905"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY71718.1"
                     /translation="MPSRFELANAIRALSMDAVQKAESGHPGAPMGMADIAEVLWNDF
                     LVHNPANPGWFDRDRFVLSNGHGSMLLYSLLHLSGYALPMEQLQAFRQFHSQTPGHPE
                     YGDTAGVETTTGPLGQGIGNAVGMALAEKMLAARYNRPGHTVIDHHTYAFVGDGCLME
                     GISHEVCSLAGTLGLGKLIALYDDNGISIDGHVEGWFTDDTARRFEAYGWQVLTDVDG
                     HDPAALKSAIETARAETVRPSLICCRTVIGKGAPNKQGTEHVHGSPLGAAEVTAAREA
                     LGWTHPPFHIPADIYAGWDARGRGAAAESAWQARLGAYRAAYPVEAAELERRMRGELP
                     PGFEADAAAYVRACQEKGETIASRKASQNAIQALAPLLPELTGGSADLAGSNLTLWKE
                     ARGVTHDDAGGNYIYYGVREFGMGAIMNGLVLHGGLRPFGGTFLVFMEYMRNAVRLAA
                     LMRIPAIYVFTHDSIGLGEDGPTHQPVEQIANLRGTPRLSTWRPGDAVESAVAWQQAI
                     LRTDGPTAMIFSRQNLPHQERTGAQLAAIARGGYVLEDCAGTPEAILIATGSELALAR
                     TAARTLNAAGRRIRVVSMPCADRFDAQDAAYRESVLPSAVRARVAIEAAHADYWYKYV
                     GLDGRVIGMTDFGASAPAGVLMQHYGFTQDNVIAVVT"
     gene            2142..3128
                     /locus_tag="PLW13_14910"
     CDS             2142..3128
                     /locus_tag="PLW13_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY71719.1"
                     /translation="MTLTATRINPPGGYENLSRLCRAAGDPLRLEILRLLARDSYGVL
                     ELCRIFGMRQPAMSHHLKVLAEAGLVYRRREGTTIFYGRHHRAPSEELEPVMQAIFAA
                     TDLVPAGTDVARGVAEVQAERAASSRGFFAANADKFRAQQELIAPRAHYEDPVAGLLA
                     ALLPQPCALALELGPGEGWLLPALAARSARVVAIDNAAAMLEQARAACAAAGLGNVEL
                     VLADSSHARTLGATADIAVANMVLHHTASPAVVLADLAAALKPGGLLLLSDLCAHDQA
                     WAREACGDLWLGFEPEALSRWAADAGLAQGESVYLALRNGFRIQVRVFHRTA"
     gene            3148..>3937
                     /gene="metK"
                     /locus_tag="PLW13_14915"
     CDS             3148..>3937
                     /gene="metK"
                     /locus_tag="PLW13_14915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQY71720.1"
                     /translation="MSEYSIFTSESVSEGHPDKMADQISDAVLDAILKDDPHARVAVE
                     TLVKTGMAIVAGEVTTNTYVDLEALIRQVILDIGYNSSDIGFDGASCAVLNAIGKQSH
                     DIAIGVDATENKEQGAGDQGLMFGYASNETDVLMPAPITYAHRLMRRQAQLRKHGVLP
                     WLRPDAKSQVTIRYEGGKPVAVDAVVISTQHAASIAHADLREAVIEEIIRPVIPPEWL
                     HAGTKYHVNPTGQFIIGGPLGDCGLTGRKIIVDSYGGMARHGGGA"
BASE COUNT          614 a         1351 c         1388 g          584 t
ORIGIN      
        1 gtcaccaccg cgatcacgtt gtcctgggtg aagccgtagt gctgcatcag cacgccggcc
       61 ggggccgagg cgccgaagtc ggtcatgccg atcacgcgac cgtcgaggcc cacgtacttg
      121 taccagtagt cggcgtgcgc ggcctcgatc gcgacgcggg cgcgtaccgc ggacggcagc
      181 acggactcgc ggtacgcggc gtcctgggca tcgaaacggt cggcgcaggg catcgagacc
      241 acgcggatgc gccgcccggc ggcgttcagc gtgcgcgcgg cggtgcgcgc cagcgccagc
      301 tccgagccgg tggcgatcag gatggcctcg ggcgtgccgg cgcaatcctc gagcacgtag
      361 ccgccacgcg cgatggccgc gagctgcgcc ccggtgcgct cctggtgcgg caggttctgg
      421 cgcgagaaga tcatcgcggt cggaccgtcg gtgcgcagga tcgcctgttg ccaggccacg
      481 gcggactcga ccgcgtcgcc ggggcgccag gtcgagaggc gcggcgtgcc gcgcaggttc
      541 gcgatctgct cgacgggttg gtgcgtggga ccgtcctcgc cgaggccgat cgaatcgtgg
      601 gtgaacacgt agatcgcggg gatgcgcatc aacgcggcca ggcgcacggc gttgcgcatg
      661 tattccatga acaccaggaa ggtgccgccg aagggacgca gccccccgtg cagcaccagc
      721 ccgttcatga tggcgcccat gccgaactcg cgcaccccgt aatagatgta attgccgccc
      781 gcatcgtcgt gcgtgacgcc gcgcgcctcc ttccacagcg tgaggttgga gccggcgaga
      841 tcggccgagc cgcccgtgag ctcgggcagc agcggggcca gcgcctggat ggcgttctgc
      901 gaggccttgc ggctggcgat ggtctcgcct ttttcctggc aggcacgcac gtaggcggcg
      961 gcatcggcct cgaagccggg gggcagctcg ccgcgcatgc gccgctcgag ctcggccgct
     1021 tcgaccgggt acgccgcgcg gtaggcgccg agacgcgcct gccaggcgga ttcggcggcc
     1081 gcaccgcggc cgcgcgcgtc ccagcccgcg tagatgtcgg cggggatgtg gaagggcgga
     1141 tgcgtccagc ccagcgcctc gcgcgcggcc gtcacttccg ccgcgccgag cgggctgccg
     1201 tgcacgtgct cggtaccctg cttgttgggc gcgcccttgc cgatcaccgt gcggcagcag
     1261 atcaggctgg ggcgcacggt ctcggcacgc gcggtctcga tcgcggactt gagcgccgcg
     1321 gggtcgtggc catcgacgtc agtgagcact tgccagccat aggcctcgaa gcgccgcgcg
     1381 gtgtcgtcgg tgaaccagcc ctcgacatgg ccgtcgatcg agatgccgtt gtcgtcatag
     1441 agcgcgatca gcttgcccag gccgagcgtg cccgccagcg aacacacctc gtgcgagatg
     1501 ccctccatga ggcagccgtc gccgacgaag gcataggtgt ggtggtcgat cacggtgtga
     1561 ccggggcggt tgtagcgcgc ggccagcatc ttctccgcca gcgccatgcc gaccgcgttg
     1621 ccgataccct gaccgagcgg gccggtggtg gtctccacgc cggcggtgtc gccatactcg
     1681 gggtggcccg gagtctgcga gtggaactgg cgaaaggcct gcagttgctc catcggcagc
     1741 gcgtagccgc tcaggtgcaa cagggaatac aggagcatgg agccgtggcc gttcgacagc
     1801 acgaaacggt cgcggtcgaa ccagccggga ttcgcggggt tgtggaccag gaagtcgttc
     1861 cacagcacct cggcgatgtc ggccatgccc atcggcgcgc cgggatgccc gctttccgct
     1921 ttttgcacgg cgtccatgct gagggcgcgg atcgcgttgg cgagttcgaa acggctgggc
     1981 atgcgggtct cctgcaggcg gctggcgcgt gaaaaggacg atattgtccc ccagcgtacc
     2041 ggtgctggaa accgccggca caccggattg gtaatccacc cgcaaactat tccatcaaat
     2101 ttttttgata tatggttgcg cccgctgcta gactgcccgc catgacgctg actgccaccc
     2161 ggatcaaccc gcccggcggg tacgagaacc tgagccgcct gtgccgcgcg gcaggcgatc
     2221 cgttgcgcct ggagatactg cgcctgctgg cgcgggactc ctacggcgtg ctcgaactgt
     2281 gccgcatctt cggcatgcgc caaccggcga tgagccacca cctcaaagtg ctggccgagg
     2341 cggggctcgt ataccggcgc cgcgaaggca ccacgatctt ctacggccgc caccaccgcg
     2401 cgcccagcga ggaactcgag ccggtgatgc aggcgatctt cgcggccacc gacctggtgc
     2461 cagccggcac ggacgtcgcc cgcggcgtgg cagaagtgca ggccgagcgc gcggcgagct
     2521 cgcgcggatt cttcgctgcc aatgccgaca agttccgcgc ccagcaggag ctgatcgcgc
     2581 cgcgcgcgca ttacgaggac ccggtggcag ggctgctggc cgcgctgctg ccgcagccct
     2641 gcgcgctcgc gctggagctc ggaccgggcg aaggctggct gctgccggcg ctcgccgcgc
     2701 gcagcgcgcg cgtggtggct atcgacaacg ccgccgccat gctcgaacag gcgcgcgcgg
     2761 cctgcgccgc ggcggggctg ggaaacgtcg agctggtgct ggccgacagc agccacgcac
     2821 gcacgctcgg cgcgaccgcc gacatcgcgg tggcgaacat ggtgctgcac cacacggcgt
     2881 ccccggcggt ggtgctcgcg gacctggccg ccgcgctgaa gcccggcggc ctgctgcttc
     2941 tgagcgacct ctgcgcccac gaccaggcct gggcgcgcga ggcctgcggc gacctgtggc
     3001 tgggtttcga gcccgaggcg ctgtcgcgct gggccgcgga cgcgggactc gcgcagggcg
     3061 agagcgtcta ccttgcattg cgtaacggat tccgcatcca ggtgcgggtt tttcatcgaa
     3121 cggcttgacc agaaaaggga tacacccatg agcgaatact ccattttcac ctccgaatcg
     3181 gtttccgagg gccaccccga caagatggcg gaccagatct cggacgcggt actggacgcg
     3241 atcctgaagg acgacccgca cgcccgtgtc gcggtggaaa cgctggtcaa gaccggcatg
     3301 gccatcgtcg ccggcgaggt tacgaccaac acttatgtcg acctcgaggc gctgatccgc
     3361 caggtcatcc tcgacattgg ctacaacagc tcggacatcg gcttcgacgg cgcctcctgc
     3421 gcggtgctga acgccatcgg caagcagtcg cacgacatcg cgatcggcgt cgacgcgacc
     3481 gagaacaagg aacagggcgc cggcgaccag ggcctgatgt tcggctacgc ctcgaacgag
     3541 accgacgtgc tgatgcccgc gccgatcacc tatgcgcacc gcctgatgcg ccgccaggcc
     3601 cagctgcgca agcacggagt gctgccttgg ctgcgcccgg atgccaagag ccaggtcacg
     3661 atccgctacg agggcggcaa gccggtggcg gtcgatgccg tcgtgatctc cacccagcac
     3721 gccgcgagca tcgcccacgc ggacctgcgc gaagcggtga tcgaggagat catccgcccg
     3781 gtgatcccgc ccgagtggct gcacgcgggc acgaaatacc acgtgaaccc gaccgggcaa
     3841 ttcatcatcg gcggcccgct cggcgactgc ggcctcaccg ggcgcaagat catcgtcgac
     3901 agctacggcg gcatggcgcg ccacggcggc ggcgcct
//
LOCUS       DAORUQ010000250         3903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_30255_length_3903_cov_5.262734, whole genome shotgun sequence.
ACCESSION   DAORUQ010000250 DAORUQ010000000
VERSION     DAORUQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30255_length_3903_cov_5.262734"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2306
                     /locus_tag="PLW13_14920"
     CDS             <1..2306
                     /locus_tag="PLW13_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQY71721.1"
                     /translation="PVYPTIASQFNDPGVNWVFHELCTRMVGKLKLDATRWAPAIDVT
                     LREPKSVALIPGARVRYLAEIAEQGRGVNAAALHQAELADRAQHLHEALRELADPLLP
                     PALVGYAPEALTAAHADNSLRVLRQRYQATLDELTADSLKLLREWPARLEGIRTPQYS
                     YTVRGKEIRGDNYKESLSRLQIPKIGAPRYSSWGELLVFLLKENLPGYYPYTAGVFPY
                     RREGEDPTRMFAGEGTPERTNRRFHFVARGQPANRLSTAFDSVTLYGEDPAVRPDIYG
                     KVGNSGVSIATVDDLKKLYSGFDLCDPSTSVSMTINGPAPMILAFFMNAAIDQQVEKH
                     LKTTGQWEAAQGRIEAYFAGRERPRYAGALPEGNDGLGLGLLGISGDKVVDAETYAAI
                     RAKTLATVRGTVQADILKEDQAQNTCIFSTGFAMKMMGDVQEFFIANDVRNYYSVSIS
                     GYHIAEAGANPISQLAFTLSNGFTIVEYYLARGMQVDDFAPNLSFFFSNGMDPEYTVI
                     GRVARRIWARAMKERYGASARSQMLKYHIQTSGRSLHAQEMSFNDIRTTLQALYALFD
                     NCNSLHTNAYDEAITTPTEESVRRAVAIQLVINRELGLNFCENPWQGSFIVEELTDLV
                     EEAVYAEFERISERGGVLGAMDTMYQRSKIQEESLYYEGRKHDGSLPLIGVNTFLPPP
                     GQEEKIEERELMRSSEEEKRQQIDNLAAFHARHAAETHEALATLQRVAEQRGNLFAEM
                     MEAVKVASLGQVSGALYEVGGIYRRNM"
     gene            2620..>3903
                     /locus_tag="PLW13_14925"
     CDS             2620..>3903
                     /locus_tag="PLW13_14925"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HQY71722.1"
                     /translation="MNDRIVRIGGASASLADSAIAFPQLLGDGALDYLVGDYLAEGSM
                     GLLAMLAQANPGGGYAPDFIPVHIGPCLAEIARQGVRVVVNAGGVNPHGCAAELARVA
                     AEQGLSPRIAVVDGDDLMGRLGEFRAAGVRDMFSGAAFPAATVDSMNAYLGAFPIAAA
                     LARGAEIVITGRVVDSALVLGPLIHEFGWTPADHDLLAAGTLAGHLLECGTQVTGGTF
                     TDWRTVPAWNRIGYPIAECRADGSFVVTKAAGTGGLVSRGTVAEQLVYEVGDPQAYFV
                     PDVSCDFSAVQLEEIGPDRVRVSGVRGRPPTASYKVCTIHRHSWRVSALQPIVGLEAA
                     AKAERQAAALIERSRRLLAQAGLGDWSRTHVEVLGTEGTLGARARHRAAREVLLRIVL
                     EHADRRAAEIFTREQHSAIVSMSPGTAIGLGNPVVP"
BASE COUNT          635 a         1325 c         1357 g          586 t
ORIGIN      
        1 ttcccgtgta tcccacgatc gcaagccagt tcaacgaccc cggcgtgaac tgggtgttcc
       61 acgagctgtg cacgcgcatg gtgggcaagc tgaagctcga cgccacacgc tgggcgccgg
      121 cgatcgacgt cacgctgcgc gagccgaaat ccgtggcgct gataccgggt gcccgcgtgc
      181 gctatctcgc ggagatcgcc gagcagggca ggggcgtgaa cgccgccgcg ctgcaccagg
      241 ccgagcttgc cgaccgcgcg cagcacctgc acgaggcgct gcgcgagctc gccgatccgc
      301 tgctgccgcc ggcgctggtc ggttacgcgc ccgaggcgct caccgccgcg cacgccgaca
      361 acagcctgcg tgtgctgcgc cagcgctacc aggccacgct cgacgagctg accgccgatt
      421 cgttgaagtt gctgcgcgag tggccggcgc gcctcgaggg catccgcacg ccgcagtaca
      481 gctacaccgt gcgtggcaag gagatccgcg gcgacaacta caaggagagc ctgagccggc
      541 tgcagatccc gaagatcggc gcgccgcgct actcgagctg gggcgagctg ctggtgttcc
      601 tgctgaagga gaacctgccc ggctactatc cctacactgc gggcgtgttc ccgtatcgtc
      661 gcgaaggcga ggatccgacg cgcatgttcg ccggtgaggg cacacccgag cgcaccaatc
      721 gtcgcttcca cttcgtggcg cgcggccagc ccgccaaccg cctttcgacg gcctttgact
      781 cggtcacgct ctacggcgag gaccccgccg tgcgccccga catctatggc aaggtcggca
      841 acagcggggt atctatcgct accgttgatg acttgaagaa gctctactcg ggcttcgacc
      901 tgtgcgatcc ctcgacctcg gtgtcgatga cgatcaacgg cccggcgccg atgatcctcg
      961 ccttcttcat gaacgccgcg atcgaccagc aggtcgagaa gcatctgaag accacgggcc
     1021 agtgggaggc cgcgcaaggg aggatcgagg cgtacttcgc cggccgcgag cgcccgcgct
     1081 acgcgggcgc gctgcccgaa ggcaacgacg ggctgggtct cgggctgctc ggcatttccg
     1141 gcgacaaggt ggtggacgcc gagacctacg cggcgattcg tgcgaagacg ctggccaccg
     1201 tgcgcggtac cgtgcaggcc gatatcctga aggaagacca ggcgcagaac acctgcatct
     1261 tcagtaccgg cttcgcgatg aagatgatgg gcgacgttca ggagttcttc atcgccaacg
     1321 atgtcaggaa ctactactcc gtctcgatca gcggctacca catcgcggag gcgggcgcga
     1381 acccgatctc gcagctcgcc ttcacgctgt cgaacggctt cacgatcgtg gagtactacc
     1441 tcgcgcgcgg catgcaggtc gatgacttcg cgccgaacct gagtttcttc ttctccaacg
     1501 gcatggaccc ggagtacacg gtgatcgggc gcgtggcgcg tcgcatctgg gcgcgcgcga
     1561 tgaaggagcg ttacggcgcg agcgcgcgca gccagatgct gaagtaccac atccagacct
     1621 cggggcgcag cctgcacgcg caggagatga gcttcaacga catccgcacc acgctgcagg
     1681 cgctctatgc cctgttcgac aactgcaaca gcctgcacac caatgcctac gacgaggcga
     1741 tcaccacgcc gaccgaggag agcgtgcgcc gtgcggtggc gatccagctc gtgatcaacc
     1801 gcgagctggg gctgaacttc tgcgagaacc cctggcaggg ctcgttcatc gtcgaggaac
     1861 tgacggacct ggtcgaggag gcggtgtacg cggagttcga gcgcatctcg gagcgcggcg
     1921 gcgtgctggg tgcgatggac acgatgtacc agcgctcgaa gatccaggag gaaagcctgt
     1981 actacgaggg caggaagcac gacggcagcc tgccgctgat cggcgtgaac accttcctgc
     2041 cgccgcccgg gcaggaggag aagatcgagg agcgcgagtt gatgcgctcg agcgaggaag
     2101 agaagcgcca gcagatcgac aatctcgcgg cgtttcacgc gcgtcacgcg gccgagacgc
     2161 acgaggcact ggcaacgctg cagcgcgtgg ccgagcagcg cggcaacctg ttcgccgaga
     2221 tgatggaagc cgtgaaggtc gcctcgctcg ggcaggtctc gggggcgctg tacgaggtgg
     2281 gcgggatcta ccggcgcaac atgtaacgcg ccgctgtgtg gcgggctgca ggtcgggcgg
     2341 taggtccagc ttcagcccga caagggttcg gcaacgccga acccgctgca gccccgctgg
     2401 caccagcgcc agcatcgcat tcatgccggc atgaatgcct gcgcatcaat tgttcgccac
     2461 ctcggcgccc tgcccgaagc ccgccgtccg ctagcccggc ctctgccctg cgaccgccac
     2521 tggcgcttcc cgccggtggc gtcgctcgcc cggccccttt aaattgacca gtcggtttga
     2581 ttaacattcc gacgaacaat tcctgccgga gccgggtcca tgaacgatcg tatcgtccgc
     2641 atcgggggtg cctcggcctc gctggccgac agtgccatcg ccttccccca gctgctgggt
     2701 gacggcgcgc tcgactacct ggtgggcgac tacctggccg agggctccat gggcctgctc
     2761 gcgatgctgg cgcaggcgaa tcccggcggc ggttacgcgc cggacttcat cccggtacac
     2821 atcgggccct gcctcgcgga gatcgcgcgc cagggcgtgc gcgtggtcgt gaacgccggc
     2881 ggggtgaacc cgcacggctg cgcggcggag ctcgcgcggg tggcggcgga acagggcctc
     2941 tcgccgcgca tcgcggtggt cgatggtgac gacctgatgg ggcgcctcgg ggaattccgc
     3001 gccgccggcg tacgcgacat gttctcgggc gcggccttcc cggccgcgac cgtggacagc
     3061 atgaacgcct acctcggcgc ctttccgatc gcagccgcgc tggcccgcgg cgcggagatc
     3121 gtgatcaccg ggcgcgtggt cgacagcgcg ctggtgctgg gcccgctgat ccacgagttc
     3181 ggctggacgc ccgctgacca cgacctgctc gcggccggca cgctcgcggg gcacctgctg
     3241 gagtgcggca cgcaggtgac cggcggcacc ttcaccgact ggcgcaccgt acccgcatgg
     3301 aaccgcatcg gctacccgat cgccgagtgc cgtgcggacg ggagcttcgt ggtcaccaag
     3361 gctgccggca ccggcggact ggtctcgcgc gggacggtcg cggagcagct ggtctacgag
     3421 gtgggcgatc cgcaggccta cttcgtgccc gacgtcagct gtgacttcag cgccgtgcag
     3481 ctggaagaga tcggccccga ccgggtgcgc gtcagcggcg tccgtggccg gccgccgacc
     3541 gcgagctaca aggtgtgcac gatccaccgc cacagctggc gcgtgagtgc gctgcagccg
     3601 atcgtgggcc tggaggccgc ggccaaggcc gaacgccagg ccgcggcgct gatcgagcgc
     3661 tcgcgccggc tgctggcgca ggccggcctc ggggactgga gccgcacgca cgtggaggtg
     3721 ctgggcaccg agggcacgct gggggcgcgg gcgcgccacc gcgccgcgcg cgaggtgctg
     3781 ctgcgcatcg tgctggagca cgccgaccgg cgcgcggccg agatcttcac gcgcgagcag
     3841 cacagcgcga tcgtctcgat gtcgccgggc accgcgatag gcctgggcaa cccggtggtc
     3901 ccg
//
LOCUS       DAORUQ010000251         3866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_30738_length_3866_cov_5.676725, whole genome shotgun sequence.
ACCESSION   DAORUQ010000251 DAORUQ010000000
VERSION     DAORUQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30738_length_3866_cov_5.676725"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PLW13_14930"
     CDS             <1..1370
                     /locus_tag="PLW13_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020193565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HQY71723.1"
                     /translation="ITFFNRGGGPITFDPLNRLDRQMNAHLFLFGPTGSGKSATLNNI
                     LNQVTAIYRPRLFIVEAGNSFGLFGDFATRLGLKVHRVKLAPGAGVSLAPFADAHRLV
                     DTPSQVQTLDADALDEDQEPAAQAASHSEELDEQRDVLGELEITARLMITGGEDKEEA
                     RMTRADRSLIRQCILDAAQSCVAEQRTVLTRDVRDALRERARDTSLPEVRRARLLEMA
                     DAMDMFCQGVDGEMFDRPGTPWPEADITIVDLATFAREGYNAQLSIAYISLINTVNNI
                     AERDQFLGRPIINVTDEGHIITKNPLLAPYVVKITKMWRKLGAWYWLATQNIDDLPKA
                     AEPMLNMIEWWICLSMPPDEVEKIARFRELNPAQKALMLSARKEAGKFTEGVILSKSV
                     EVLFRAVPPSLYLALAQTEPEEKAERFQLMQQYGIGELDAAFKIAEKIDRARGIESLA
                     LDALG"
     gene            1382..2137
                     /locus_tag="PLW13_14935"
     CDS             1382..2137
                     /locus_tag="PLW13_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQY71724.1"
                     /translation="MRITRFVPSRSVALVAVLLVVALIATVWLGMQPQAPIAEGASTP
                     AEFNPAPTAAPDATAGPPWRYGRADARFTVVEYADLECPFCRAYFAVLKQWIDAHPEV
                     NWQWHHLPLTTHEPAATANARLVECVGEAGGAAAFWQAVEWVYTHTRGDGQGLPEGLG
                     YPGITPAAQQCLDSDRPDTLIRAQSASAAQEGIKVTPTLRLQDRQSGKTLLLHGPVEG
                     DALLSAIDLLAAGGTAETASKEMPAESLGDMSR"
     gene            2330..2908
                     /locus_tag="PLW13_14940"
     CDS             2330..2908
                     /locus_tag="PLW13_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71725.1"
                     /translation="MQPKTHVVDGVTFVATPYRAGTFIREDVFWRQFTITCQGQVEYP
                     EDDPTYGTYWVPSILELGERGLIEDAMALALACVSEHEFDLNPDSDALDFRPELVLVR
                     DSLNRLVLTGQVWGAGIRWSEPITSDEEAAAVAKQVDDLRGEASYEAGWDNYSTAEGL
                     RLRARVLAGRLVDPFWQAHARRAVQASTAAVA"
     gene            3051..3497
                     /locus_tag="PLW13_14945"
     CDS             3051..3497
                     /locus_tag="PLW13_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HQY71726.1"
                     /translation="MTAPLFNSIPQRRATRIACAGGLLLALCGQVASAADVLVVTDSR
                     HPVQSTAGARVVELDLPERIEAELAAGLPNDPSRAAALVQQRLRDGGQALQRRIGSAY
                     QGVADAWGLGIAKVPAVVVDRRYVVYGEPDVARAVARIEAHRRTQP"
     gene            3494..>3866
                     /locus_tag="PLW13_14950"
     CDS             3494..>3866
                     /locus_tag="PLW13_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HQY71727.1"
                     /translation="MKRLLSASSRRARLAIASVLLGGAASSFALNTATIVSSALSPDC
                     IEYRVVGICYWLFCTTFGCSVRTSVKVRHYVPDAVVSSYSNTGQNPWVEVRAMSAPNT
                     TAQAGGDGTTNHDNENNLAKFK"
BASE COUNT          615 a         1364 c         1278 g          609 t
ORIGIN      
        1 gcatcacgtt cttcaaccgc ggcggcgggc cgatcacctt cgacccgctg aaccgcctcg
       61 accggcaaat gaacgcgcat ctcttcctgt tcggccccac cggctcgggc aagagcgcca
      121 cgctcaacaa catcctgaac caggtcacgg cgatctaccg gccgcgcctg ttcatcgtgg
      181 aggccggcaa cagcttcggc ttgttcggcg acttcgccac gcggctgggc ctcaaggtgc
      241 atcgcgtgaa gctcgccccc ggcgctggcg tcagcctcgc gccgttcgcc gacgcgcacc
      301 ggctggtcga tacgcccagc caggtgcaga cgctggatgc cgatgcgctg gacgaggacc
      361 aggagcccgc cgcgcaggcg gcaagccaca gcgaggaatt ggacgagcag cgcgacgtgc
      421 tcggcgagct ggagatcaca gcgcggctga tgattaccgg cggcgaggac aaggaggaag
      481 cacggatgac gcgcgccgat cgcagcctga tccgccagtg catcctcgat gcggcgcaga
      541 gctgcgtagc cgagcaacgc accgtgctca cgcgcgacgt gcgcgatgcc ctgcgcgagc
      601 gcgcccgcga cacgtcgctg cccgaggtgc ggcgcgcgcg gctgctggag atggcggacg
      661 cgatggatat gttctgccag ggcgtggacg gcgagatgtt cgaccggccc ggcacgccct
      721 ggcccgaggc cgacatcacc atcgtggacc tcgccacctt cgcgcgcgag ggctacaacg
      781 cgcagctctc catcgcctac atctcgctga tcaacacggt caacaacatc gccgagcggg
      841 atcaattcct cggccggccg atcatcaacg tgacggatga aggccacatc atcaccaaga
      901 acccgctgct tgcgccctac gtcgtgaaga tcacgaagat gtggcgcaag ctcggcgctt
      961 ggtactggct ggcgacgcag aacatcgacg acctgcccaa ggccgccgag cccatgctca
     1021 acatgatcga gtggtggatc tgcctctcca tgccaccgga cgaggtggag aagatcgcgc
     1081 gcttccgcga gctgaacccg gcacagaagg cgttgatgct gtcggcccgc aaggaggcgg
     1141 gcaagttcac cgagggcgtg atcctctcca agagcgtgga ggtgctgttc cgtgcggtgc
     1201 ctcccagcct ctacctggcg ctggctcaga ccgagccgga agagaaggcg gaacgcttcc
     1261 agttgatgca gcagtacggc attggcgagc tggatgcggc cttcaagatc gccgagaaga
     1321 tcgaccgcgc gcgcggcatc gagtcgctgg cgctcgatgc gctcgggtga cggagaacac
     1381 catgcggatc actcgcttcg ttccatcccg ctccgtcgca ttggtcgccg tgctgctcgt
     1441 ggttgccctg atcgcgacgg tctggctcgg catgcagcca caggcgccga tcgccgaagg
     1501 ggcttccacg ccggccgagt tcaatcccgc gcccacggcg gcacctgatg ccacggccgg
     1561 cccgccgtgg cgctacggac gcgccgatgc acgcttcacg gtggtggagt acgccgacct
     1621 cgaatgtccg ttctgccgcg cctacttcgc ggtgctaaag cagtggatcg acgcgcaccc
     1681 cgaggtgaac tggcagtggc accacctgcc gctgacgacg cacgagccgg ccgcgaccgc
     1741 caatgcgcgc ctggtcgagt gcgtcggcga agccggcggc gcggccgcgt tctggcaggc
     1801 ggtcgaatgg gtctacacgc acacccgcgg cgacggccag ggcctgcccg agggcctggg
     1861 ctatcccggc atcacgccgg ccgcccagca gtgcctcgac agcgaccgcc ctgacacgct
     1921 gatccgcgcg cagtcggcga gcgcggcgca ggagggcatc aaggtcacgc ccacgctgcg
     1981 cctgcaggat cgccagtccg gcaagacgct gctgctgcat ggcccggtcg aaggcgatgc
     2041 gctgctgtcg gccatcgacc tgctggcggc cggcggcacc gccgaaaccg catcgaagga
     2101 aatgcctgcc gagtcactcg gcgacatgtc caggtagccc cgatcttcaa ggctacgccg
     2161 cggccccggc cgcgctgatc gaccccgttc gcttcgcatc ctggacgcga acagccgccg
     2221 cgctgcggtg gtggatgcct gttcttcctt cgcttgttgt tcatcccacg cgggtcgccc
     2281 acgcccgcag gggcggcatg gcgttcccgc accttctttg gagactgcca tgcaacccaa
     2341 gacccatgtt gtcgatggcg tgaccttcgt ggccacgcct taccgcgccg gaaccttcat
     2401 ccgcgaggat gtgttctggc gccagttcac catcacctgc caaggccagg tcgagtaccc
     2461 cgaggacgat ccgacctacg gcacctactg ggtgccttcc atcctcgaac tcggcgaacg
     2521 cggcctcatc gaggacgcga tggcgctggc cctggcctgc gtgtcggagc acgagttcga
     2581 cttgaacccg gacagcgacg cgctggactt ccggccggag ctggtcctcg tgcgcgattc
     2641 cctgaaccgc ctcgtgctca ccggccaggt gtggggtgcc ggcatccgtt ggtccgagcc
     2701 catcacctca gacgaggaag cggccgccgt cgccaagcag gtcgatgatc tgcgcggcga
     2761 ggcctcctac gaggccggct gggacaacta cagcacggcc gaaggcctgc gcctgcgtgc
     2821 ccgcgtgctc gccggccggc tggtcgatcc gttctggcag gcccatgccc gccgcgccgt
     2881 tcaagcctca acggctgcgg tcgcctgatc cccacgggag ctttggctcc cgttcttctt
     2941 ccgtgcgctg cttgcgaaag cggggtgtgc gcggtgcgca ttccttgtcg cagcggacat
     3001 gccttcggcg ttcgactagg ggtcttgctt tcattcccag gacgcccgcc atgacggccc
     3061 ccttgttcaa ttccatcccg cagcggcggg ccacgcgcat cgcgtgcgcg ggagggttgc
     3121 tgcttgccct gtgcggccag gtcgcttctg ccgccgacgt gctggtcgtg accgacagcc
     3181 gccatccggt gcaatccacg gccggcgcgc gcgtggtcga gctggacctg cccgagcgca
     3241 tcgaggccga gctggcggcc ggcctgccca acgaccccag ccgcgccgcc gcactcgtgc
     3301 agcagcggct gcgcgatggc ggccaggcct tgcaacggcg catcggcagc gcttaccagg
     3361 gtgtggccga tgcctggggg ctgggcatcg ccaaggtgcc cgccgtggtg gtcgaccgcc
     3421 gctacgtggt ctacggcgag cccgacgtgg cgcgcgccgt cgcccgcatc gaagcccatc
     3481 ggaggacgca gccatgaaac gcctgctgtc ggcatcgtcg cgccgcgcgc gccttgccat
     3541 cgcttcggtg ctgctgggcg gcgcggcatc gagcttcgcc ctgaacaccg ccaccatcgt
     3601 gtcctcggcc ctgtcgcccg actgcatcga gtaccgcgtc gtcggcatct gctactggct
     3661 gttctgcacc acgttcggct gctcggtgcg cacgtcggtg aaggtgcggc actacgttcc
     3721 ggacgcggtg gtatcgagct acagcaatac cgggcagaac ccgtgggtcg aggtgcgtgc
     3781 gatgagcgcg ccgaacacca cggcgcaggc cggcggcgac ggcacgacga accacgacaa
     3841 cgagaacaac ctcgccaagt tcaaga
//
LOCUS       DAORUQ010000252         3856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_30867_length_3856_cov_4.606419, whole genome shotgun sequence.
ACCESSION   DAORUQ010000252 DAORUQ010000000
VERSION     DAORUQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30867_length_3856_cov_4.606419"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1229
                     /locus_tag="PLW13_14955"
     CDS             297..1229
                     /locus_tag="PLW13_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004664319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY71728.1"
                     /translation="MSNALTIRQLRKTYANGFAALKGIDLDVAQGDFFALLGPNGAGK
                     STTIGVICSLVQKTGGTVEVFGTDIDANFPLAKRHLGVVPQEFNFNHFEKVGDILVTQ
                     AGYYGLPLALARERALKYLHRLDLWDKREQPARNLSGGMKRRLMIARALVHEPRLLIL
                     DEPTAGVDIELRRSMWGFLQEINAAGTTIILTTHYLEEAESLCRNIAIIDHGEIVENT
                     SMKQLLRTLDRETFVLDVLGDVPANFTVEGYLVRVVDVHTLEVTVDRHEALNGLFERL
                     SAKGIAVSSMRNKANRLEELFVALLHRNKPGEAA"
     gene            1226..1999
                     /locus_tag="PLW13_14960"
     CDS             1226..1999
                     /locus_tag="PLW13_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71729.1"
                     /translation="MNRHELFMAFLTILVKEVRRFVRIWPQTLVPPAITVALYYVIFG
                     SLIGSRIGPMGGFDYMQFVVPGLIMMAVITNSYANVVSSFFGAKFQHSIEELLVSPTP
                     NWVILLGFTIGGVARGLCVGLIVTVLSLAFTQLHVEHPLLTVLVVFLTAVLFSMAGLI
                     NAVFANSFDDISIVPTFILTPLTYLGGVFYSIDLLPPFWRAVSQVNPILHMVNAFRYG
                     VLGVSDVHVGTALGMICLFIAAGWFYCLHLLNSGKRLRQ"
     gene            2084..2917
                     /gene="queF"
                     /locus_tag="PLW13_14965"
     CDS             2084..2917
                     /gene="queF"
                     /locus_tag="PLW13_14965"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485276.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HQY71730.1"
                     /translation="MATDKFGDTPLGRRIEAVSQYTPSLLFPIERLRLRERLLLDPQQ
                     LPFEGADLWTAWELTWIDRDGKPEVGVAQLRVPCNSRAVFESKSLKLYLQSFSQTAFA
                     SAYDVVRTLESDLSVTAGAPVVVDLLSMSQALQAGVASFPGECLDTLKISASMYRPDP
                     GLLDVTRDGRVASESLYSNLLRSLCPVTGQPDIGSIQVTYTGTRIDHEALLRYIISFR
                     EHQGFHEQCVERIFLDIMDRCRPRELTVYARYCRRGGIDINPCRSTEKGIPPHLRLVR
                     Q"
     gene            3025..3115
                     /locus_tag="PLW13_14970"
     tRNA            3025..3115
                     /locus_tag="PLW13_14970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3059..3061,aa:Ser,seq:gga)
     gene            complement(3298..>3856)
                     /locus_tag="PLW13_14975"
     CDS             complement(3298..>3856)
                     /locus_tag="PLW13_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71731.1"
                     /translation="DQAKALATGWSDGTMPQRIAQLQQWRPRITIGRDALRIELPGES
                     VRTVWGARMTAAATRSPAPIEWTVPVALKRSGIETKLVIPSAPPPLAHTASATALREA
                     VEKALTWNQALLADRVKSTCSLAKAEGVTQRFIAQRLQLAWLAPDIIQRIIAGDIPDT
                     LTLEHFKKRRIPLDWAEQRAFFQIT"
BASE COUNT          635 a         1264 c         1262 g          695 t
ORIGIN      
        1 cggctgacgc cactgccgac atggcgagcg acgcagcgga cgcaaccgcc gatgccgcgg
       61 aagccaccgg cgaagccgtg gacgcggcag ccgacgctgc caaggaagct gccgacgcag
      121 cagcccaggc cgccaaggac gcgatgaaac agtaagctcg cgatcgcgag taaagccggg
      181 cagcaatgcc cgcatgcgcg gaccggcact ggctgccggt ccgcgattct cctctccctg
      241 cgaccactcc ctgaattgcg gatattccgc gccacgccga ggagtcatgc cccgcgatga
      301 gcaatgccct gactatccgc cagctccgca agacctacgc caacggcttc gccgcgctga
      361 agggcatcga tctggacgtg gcgcagggtg atttcttcgc gctgctggga cccaacgggg
      421 cgggcaagtc gaccacgatc ggcgtgatct gctcgctggt gcagaagacc ggcggcaccg
      481 tggaagtctt cggcaccgac atcgacgcca acttcccgct cgccaagcgc cacctcggcg
      541 tggtgccgca ggaattcaac ttcaaccact tcgagaaggt gggcgacatc ctggtcacgc
      601 aggccggcta ctacggcctg cccctggcgc tggcgcgcga gcgcgcactg aaatacctcc
      661 atcgcctgga tctgtgggac aagcgcgagc agccggcgcg caacctctcc ggtgggatga
      721 agcggcggct gatgatcgcg cgcgcgctgg tgcacgagcc gcggctgctg atcctcgacg
      781 agcccacggc gggggtcgac atcgagctgc gccgctcgat gtggggtttc ctgcaggaga
      841 tcaacgccgc cggcaccacg atcatcctga ccacgcacta cctcgaggag gccgagagcc
      901 tctgccgcaa catcgcgatc atcgaccacg gcgagatcgt cgagaacacc agcatgaagc
      961 agctgctgcg cacgctggac cgcgagacct tcgtgctcga cgtgctcggc gacgtgcccg
     1021 cgaatttcac ggtcgagggc tatctcgtgc gcgtggtcga cgtccacacc ctggaggtca
     1081 cggtcgaccg ccacgaggcg ctgaacggct tgttcgagcg actgtcggca aagggcatcg
     1141 cggtgagcag catgcgcaac aaggccaacc ggctcgagga gctgttcgtg gcgttgctgc
     1201 accgcaacaa gccgggggaa gcggcatgaa ccgccacgag ctgttcatgg ccttcctgac
     1261 catcctggtc aaggaggtgc gccgcttcgt gcgtatctgg ccgcagaccc tggtgccgcc
     1321 ggcgatcacg gtggcgctgt actacgtgat cttcggcagc ctgatcggct cgcgcatcgg
     1381 cccgatgggc ggcttcgact acatgcagtt cgtggtgccc gggctgatca tgatggcggt
     1441 gatcaccaac tcctacgcca acgtggtgtc ctcgttcttc ggcgccaagt tccagcacag
     1501 catcgaggag ctgctggtat cgccgacccc gaactgggtg atcctgctcg gctttaccat
     1561 cggcggtgtc gcgcgcgggc tctgcgtggg attgatcgtc accgtgctct cgctcgcctt
     1621 cacgcagctg cacgtcgagc acccgctgct caccgtgctg gtggtgttcc tcacggcggt
     1681 gttgttctcg atggccgggc tgatcaacgc cgtgttcgcc aacagcttcg acgacatctc
     1741 gatcgtcccg acattcattc tgacgccgct cacgtatctc ggcggcgtgt tctattcgat
     1801 cgacctgctg ccgccgttct ggcgcgcggt gtcgcaggtg aacccgatcc tgcatatggt
     1861 gaacgccttc cgctacggag tgctgggcgt gtcggacgtg catgtgggca cggcgctggg
     1921 gatgatctgc ctgttcatcg cggccggctg gttctactgc ctgcacctgc tgaattccgg
     1981 caagcgcctg cgccagtgag cgcggcatcc gggggtccgg acggcagggg aaattcgtct
     2041 acacttgggc gggtgtgatc accggtcgct ggcggtattg gcaatggcaa cagacaagtt
     2101 cggcgacacc ccgctgggac gcaggatcga agcggtgtcg cagtacaccc cctcgctgct
     2161 gttcccgatc gagcgcctgc gcctgcgcga gcgcctgctg ctggatccgc agcaactgcc
     2221 cttcgagggc gcggacctgt ggaccgcgtg ggagctcacc tggatcgatc gcgacggcaa
     2281 gcccgaggtc ggcgtggcgc agctgcgcgt gccctgcaac tcgcgcgcgg tgttcgaatc
     2341 gaagtcgctg aagctctacc tgcagtcgtt cagccagacc gccttcgcca gcgcctacga
     2401 cgtggtgcgc acgctggaat cggacctctc ggtgaccgcg ggcgcccccg tggtggtgga
     2461 cctgctgtcg atgagccagg cgttgcaggc cggtgtggcc agctttcccg gcgagtgcct
     2521 ggacacgctg aagatctccg cctcgatgta ccgccccgac cccggcctgc tcgacgtgac
     2581 gcgcgatggc cgcgtggcga gcgagtcgct ctacagcaac ctgctgcgct cgctgtgccc
     2641 ggtgaccggg cagcccgaca ttggcagtat ccaggtcacc tacaccggca cgcgcatcga
     2701 ccacgaggcg ctgctgcgct acatcatctc gttccgcgag caccagggct tccacgagca
     2761 gtgcgtggag cgcatcttcc tcgacatcat ggaccggtgc cgcccgcgcg agctcacggt
     2821 ctatgcccgc tactgccgtc gcggcggcat cgacatcaac ccctgccgca gcacggagaa
     2881 gggcatcccg ccgcacctgc gcctggtgcg ccagtagcct gcgggaacgt gaaatgctgg
     2941 cgctcctcgc ggcttgtaag ggcagggcca gttctataca gtacgcgcct cgttttgccc
     3001 gccgcggcag agcatccagg ttccggtgag gtgtccgagt ggttgaagga gcacgcctgg
     3061 aaagtgtgta tacgttaata gcgtatcgag ggttcgaatc cctccctcac cgccaaacag
     3121 tctgctccga atccacatcg ttggagacga gcagggaatg gccggaaaag cccgcaaatt
     3181 gcgggctttt tcgtatctgg atgatgccgt gacagcgcac cgcaacatgg cgtcacttcg
     3241 cgcttttgcc cgaaagtctc caaacgggac tttgccggtt cgctgtcggg aaactggtca
     3301 ggtgatctgg aaaaaagcgc gctgttccgc ccaatcgagc gggatgcggc gcttcttgaa
     3361 atgctcgagg gtgagcgtgt cgggaatgtc gcccgcgatg atgcgctgga tgatgtcggg
     3421 cgccagccag gccagttgga gacgctgtgc gatgaagcgt tgcgtgacgc cctcggcctt
     3481 cgccagggag caggtcgact tgacccggtc agccagcagg gcctggttcc aggtgagtgc
     3541 cttctcgacg gcttcccgca gcgcggtggc cgaggcggtg tgagccaggg gaggcggagc
     3601 ggacggaatc accagtttgg tttcgatccc gctgcgtttc agggcgacgg ggacggtcca
     3661 ctcaatcggg gcgggtgacc gtgttgctgc tgctgtcatt cttgcgcccc acaccgtgcg
     3721 taccgattcg cccggcagtt cgatgcgcag tgcatcgcgc ccgatggtga tccgtggccg
     3781 ccactgctgc agttgcgcga tgcgctgcgg catcgtcccg tcgctccacc ccgttgccag
     3841 cgccttggcc tgatcc
//
LOCUS       DAORUQ010000253         3826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_31280_length_3826_cov_9.316097, whole genome shotgun sequence.
ACCESSION   DAORUQ010000253 DAORUQ010000000
VERSION     DAORUQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31280_length_3826_cov_9.316097"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3826
                     /locus_tag="PLW13_14980"
     CDS             <1..>3826
                     /locus_tag="PLW13_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HQY71732.1"
                     /translation="ARAESGPAQLSFAQQRLWMLDQIEPGGTAYVISGALELRGALDA
                     RALERAFEAIVNRHDSLRTVFVNVDGEPRQVVSEPGPWALAVEDLGGEGNARERLDER
                     LRTQAARGFDLARGPLFRARLYRLAMDRHVLLLNMHHIVSDGWSLGVLTRELGEFYRS
                     FVRGEAPGLPALRIQYRDFARWQRGWLQGEVLASQISYWRESLAGAPRVLELPTDRPR
                     PAVESHRGASHAFTLPRELADQLQALSRREGSSLFMTLLSGFALLLSRYSGQRDLLIG
                     TPVANRNRAEIEPLIGFFVNTLVLRADLSGEPSVRAFLARMREVCLDAFAHQDLPFDR
                     LVEELHPERDMSRNPVFQVMFGLQNAPMDPLELPGLTFEPVNAAAGAAQFDISLMAWE
                     TPEGLAAAFTYATDLFDAATIARMGVHWGALLEAMVAAPERRVQELAMLTGAERRQLL
                     VEWNDTKTDYPRESCVQELFEAQAARTPDAVAVAYRNQRLTYRELSARSSQLAHFLRE
                     RGVGPDTLVPICFERSPEMVIGVLGILKAGGAYVPLDPGYPADRLDFMLEDTQASILL
                     TQSHLAPNLPGHGTTAMCLDTEWHELERFAPKSPPSRTNATHLAYVIYTSGSTGRPKG
                     VCVTHRGLVNYVCYATAAYEVSEGAGAPVHSSIAFDLTVTSLFAPLLAGRTVHLLPDD
                     LGVEALVNAFQDAAGEYSLVKITPAHLQVLQLALSAEQAAKRTRVFVIGGEQLEAEAI
                     EFWRRHAGNTVLVNEYGPTETTVGCCTYSVTAESRVSGAIPIGRPIANTQLYILDDCM
                     EPVPIGVIGELYIGGEGVARGYLRRPDLTAEKFVADRFSGVPQARLYRTGDLVRYLPD
                     GNIEFIGRCDNQVKLRGYRIELGEIETVLYQHPAIREVVVLAREDVAGDKRLVAYLVA
                     REAAPSAAELKEHLMRTLPQHMVPSAFVFLQEMSLTPNGKVDRKALPAPERLLGAEAI
                     YVAPRTSTEEIVAGIWAEVLKLERVGVEDNFFELGGHSLLAVRVIERMRRAGLHAQVR
                     TLFMAATVAELAAAAGGESGVVEVPPNRIAPGCEVIVPDMLPLVQLAPGDIERIVEEV
                     PGGAANVQDIYPLAPLQEGILFHHLMAGEGDPYLLHFLVGFGKRAQLDGYLRALRSVI
                     GRHDILRTAVLWEGLPEPVQVVWREAPLVVEEISLDGAGGDVAEQLRARFDPRRYRLD
                     VRKAPLLRVFIAHDAARARWVMLLLCHHLTVDHTTAEVMQQEIQAHLLGRAGELPAPL
                     PFRNFVAQARLGI"
BASE COUNT          567 a         1211 c         1373 g          675 t
ORIGIN      
        1 ggcgagggcg gagtccggcc cggcgcagct ctcctttgcc cagcagcggc tgtggatgct
       61 ggaccagatc gagccgggtg gcacggccta cgtcatttca ggggcgctcg agctgcgcgg
      121 cgcgctggat gcgcgggcgc tggagcgcgc gtttgaagcg atagtcaatc gccatgattc
      181 gttgcgcacg gtgttcgtga acgtggacgg cgaaccgcgg caggtcgttt cggagccggg
      241 gccgtgggcg cttgcggtcg aggacctggg cggggagggg aacgctcgcg agcggctcga
      301 cgagcgcctt cgcacgcagg ctgcgcgcgg gttcgatctg gcgcgcggac cgctgttccg
      361 cgctcggctc taccggctgg cgatggaccg ccacgttctc ctgctgaaca tgcatcacat
      421 cgtctcggac ggctggtcgc tgggggtgct cacgcgcgag ctgggcgagt tctaccgcag
      481 ctttgtgcgc ggcgaggcgc ccgggcttcc ggcgctgcgc attcagtatc gtgacttcgc
      541 gcgctggcag cgcggctggc tgcagggcga ggtgctggcg tcccagattt cgtactggcg
      601 tgagagcctg gcgggtgcgc cccgggtgct cgaattgccc acggatcggc cgcgtcctgc
      661 cgtggagagc caccgcgggg cgtcccatgc gttcacgctc ccccgggagc tggctgacca
      721 attgcaggca ctttcgcggc gcgaggggag cagtcttttc atgacgctcc tgtccggttt
      781 cgcgctgctg ctttcgcgct acagcgggca gcgcgatctc ctgatcggca cgccggtggc
      841 caaccgcaac cgtgccgaga tcgagcccct gatcggcttc ttcgtgaaca cgctggtgtt
      901 gcgcgcggat ctctccggcg agccttcggt gagggcgttc ctggctcgga tgcgcgaggt
      961 gtgcctggac gcgttcgcgc accaggacct gcctttcgac cggctggtcg aggagctgca
     1021 ccccgagcgc gacatgagcc gcaaccccgt gttccaggtg atgttcgggc tgcagaacgc
     1081 gccaatggat cccctcgagc tgccgggact caccttcgag cccgtgaacg cggctgcggg
     1141 ggcggcgcag ttcgacatct cgctgatggc atgggagacg ccggagggac ttgccgccgc
     1201 ctttacctac gcgaccgacc tcttcgatgc cgcgacgatc gcgcgcatgg gcgtgcactg
     1261 gggcgcgctg ctcgaggcga tggtggccgc acccgagcgc cgcgtgcagg agctggcgat
     1321 gctgaccggg gccgagcgcc ggcagttgct cgtcgagtgg aacgacacga agacggacta
     1381 tccgcgcgag agttgtgtcc aggagttgtt cgaagcgcag gctgcgcgca cgcctgacgc
     1441 ggtggcagtg gcatacagga accagcgcct tacctaccgc gagctgagtg cccgttccag
     1501 ccaactcgcc cacttcctca gggaacgcgg cgtggggccc gacacgctgg tcccgatctg
     1561 tttcgagcgt tccccggaaa tggtgatcgg cgtgctcggg atcctcaagg caggcggcgc
     1621 ctacgtgccg ctggacccgg gctaccccgc cgatcgactg gacttcatgc tcgaggacac
     1681 ccaggcgtcg attctgctca cccaatcgca cttggctccg aacctccccg ggcatggcac
     1741 caccgcgatg tgcctcgaca ccgaatggca cgagctcgaa cgttttgcgc cgaaatcgcc
     1801 gccgagccgc acgaacgcga cgcatctcgc ctatgtcatc tacacctcgg gatccacggg
     1861 caggccgaaa ggggtgtgcg tcacgcaccg gggtctcgtc aactatgtgt gctacgccac
     1921 ggctgcctat gaagtttccg agggcgctgg agcgccggtg cattcgtcca tcgccttcga
     1981 tctgactgtc acgagccttt tcgctccgct gctcgcgggc cgcacggttc atctcctgcc
     2041 ggacgacctg ggcgtcgagg cgctcgttaa cgcgttccag gacgctgccg gcgaatacag
     2101 cctggtcaag atcacgccgg cgcacttgca ggtcctgcag ctggcgctgt cggcggagca
     2161 ggcggcgaag agaacgcggg tgtttgtcat cggcggggag cagctggagg cggaggccat
     2221 agagttctgg cgccggcatg cgggcaacac ggtgctcgtc aacgaatatg gtccgacgga
     2281 aacgacggtg gggtgctgca cgtattcggt gacggctgag tctcgagtga gcggagcgat
     2341 ccccatcggc cgacccatag ccaacacgca gctctacatt ctcgacgatt gcatggagcc
     2401 tgtcccgatc ggggtaatcg gcgagttgta catcggtgga gagggcgtgg cgaggggcta
     2461 tctccggcgg cctgatctca cggccgagaa gttcgttgcc gatcggttca gcggcgttcc
     2521 ccaggcgcgg ctctaccgca cgggggacct ggtgcgctat ctccccgacg gcaacatcga
     2581 gttcatcggc cgctgcgaca accaggtgaa gctgcgcggc taccgcatcg agctgggcga
     2641 gatcgagacg gtcctgtacc agcaccccgc cattcgcgag gtggtcgtgc ttgcgcgaga
     2701 ggatgtggcc ggggacaagc gactggtggc ctacctggtg gcgcgggagg cggcaccctc
     2761 ggccgcggaa ctcaaggaac acctgatgcg gacgttgccg caacacatgg tgccgagcgc
     2821 cttcgttttc ctgcaggaga tgtcgttgac gcccaacggc aaggtggacc gcaaggcact
     2881 gccggcgccg gagcgtctcc tgggcgcgga ggcgatctac gtcgccccgc gcacctcgac
     2941 cgaggagatc gttgccggca tctgggcgga ggtgctcaag ctcgagcgcg tcggcgtgga
     3001 ggacaacttc ttcgagctgg gcgggcattc gttgctcgcg gtgagagtga tcgagcgcat
     3061 gcgccgcgcg gggcttcatg cgcaggttcg cacgctcttc atggcggcca cggtggccga
     3121 gctggcggcc gcggcgggcg gcgagagcgg cgtggtcgaa gtgccgccca accggattgc
     3181 accgggatgc gaggtgattg tcccggacat gctgccgctg gttcagctcg cgcccgggga
     3241 catcgagcgc atcgtcgaag aggtgcccgg tggtgcggcc aatgtccagg acatctatcc
     3301 gctcgcgccg cttcaggagg ggatcctttt ccaccacttg atggcgggcg agggcgaccc
     3361 ctatttgctt catttcctgg tcggcttcgg caaacgcgcc cagttggacg ggtacttgcg
     3421 ggccctgcgg tcggtgatcg ggcgccacga catcctgcgc acggcggtgc tgtgggaggg
     3481 gctgcccgag ccggtgcagg tggtgtggcg cgaggccccg ctcgtggtgg aggaaatcag
     3541 cctcgatggg gccggcggtg atgtcgccga gcagttgcgc gcgcggttcg atccgcgccg
     3601 ttaccggctc gacgtgcgca aggcgccgct gctgcgggtg ttcatcgcgc acgatgcggc
     3661 aagagcgcgc tgggtgatgt tgctcctgtg tcatcacctg acggttgacc acaccacggc
     3721 ggaagtcatg cagcaggaga tccaggcgca cctgctgggt cgagccgggg agctgcccgc
     3781 gccgttgccg tttcgcaact tcgtggcgca ggcgcggctg ggcatt
//
LOCUS       DAORUQ010000254         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_31380_length_3818_cov_6.725485, whole genome shotgun sequence.
ACCESSION   DAORUQ010000254 DAORUQ010000000
VERSION     DAORUQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31380_length_3818_cov_6.725485"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLW13_14985"
     CDS             <1..1113
                     /locus_tag="PLW13_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71733.1"
                     /translation="YDAPLGDFFAGGLLVRDFAEHKFHRRIMQTAFKTSSMQHYVHSV
                     NEISAATVERWGKESSGFRYYPHIKELLLDVGARVFVGVNLDNEIAPLNKAFLDMMAG
                     TLAIVRKDWPGLGLVYHRGMNGRRYLEQYFQGLVPQRRRDEGMDMMSHFSKETTEEGQ
                     FFPDKVVADHLIFLVLAAHDTTTSALTMASYYLARHPEWQERLRGEVLGLDRSLAYDD
                     LGANVPLLEQTFHEILRMHPPVPQLMRRTIRETELGGYRIAPDTMVAISPIFSHRMPE
                     YWKDLHRFDPERFGPARQEHKQHPYLWVPFGGGAHKCIGLHFADMLFKCVMTEMLRRF
                     RFSLPVNYPDPPSIVHFPFAKLTDDLPLVLEPLGRA"
     gene            1106..1720
                     /locus_tag="PLW13_14990"
     CDS             1106..1720
                     /locus_tag="PLW13_14990"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HQY71734.1"
                     /translation="MPERIARLLAALAFALGVAADVSALETGEALPALRIEELGELQL
                     AGETIRFAPWQSGALTGKVQVLQYLAARMSAKSLNEPFTDTLRDSGIPIERYHLTTIV
                     NLDDALIGTRGMVLSELESNKKRYFRSSIVADAHGLGREAWQLGPKSSAIIILAPDGS
                     VRFFRDGPMNSADIAQAMRLVRCGAVEQLALLAPELQSGCDGAR"
     gene            1749..3038
                     /locus_tag="PLW13_14995"
     CDS             1749..3038
                     /locus_tag="PLW13_14995"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY71735.1"
                     /translation="MSSIALDFPRRALRNVMHRIEVPRDIGAVTHIDSASECDPLEAG
                     IAHGQAAAIWRAVEDLYRTGYYPAVMFCLRREGKVVFNRALGHARGNGPEDDAGTPKL
                     PATASTPSCIFSASKAITAMVLHRMEEKGEINLLNPISHYIPEFARHGKERTTIYHLL
                     SHRAGIPGIANVDDPRVVLDHEQSLQLLCEAQPQHLFGREQAYHAITGGVILAEIVRR
                     VSGMDIRKAWRTWFKEPMGLKVLDYGASAPVRAKIAGQALTGIQGCRLVDDFARRAIG
                     ATFADVMELLATADFYKAVIPSGNMVSTAEEASAFYQMLLDGGRWNGRQILRPETIQR
                     ATMEVGPHVLDRSIGMPLRFSQGFMLGGAPLGLFGQRSQHAFGHIGLANNITWADPER
                     QISVALLVSGIPVLAGNYVALLKLIARIGGACPRVEN"
     gene            3043..3807
                     /locus_tag="PLW13_15000"
     CDS             3043..3807
                     /locus_tag="PLW13_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQY71736.1"
                     /translation="MCVRTERCGRVATIVIDRPACRNAVDGPTAAALAGAFRDFDADD
                     SLDIAVLWGAGGTFCAGADLKAVAQEPARANRVAPDGDGPMGISRLRLNKPVIAAVAG
                     HAVAGGLELAAWCDLRVAEESAVFGVFCRRWGVPLIDGGTVRLPRLIGMSHALDMILT
                     GRAVGAPEALAMGLANRVVPDGTAREAAEALAAEIARFPQACLRADRLSALEQAGLPV
                     EEAMRNEFARGAAIAAMPEMREAAARFTGGAGRHGT"
BASE COUNT          604 a         1305 c         1311 g          598 t
ORIGIN      
        1 tacgacgcgc cgctcgggga cttcttcgcc ggcggcctgc tggtgcgcga tttcgccgag
       61 cacaagttcc accgtcgcat catgcagacc gcgttcaaga ccagctcgat gcagcactac
      121 gtgcacagcg tcaacgagat ctcggcggca accgtggagc gctggggcaa ggaaagcagc
      181 ggtttccgct actacccgca catcaaggag ctgctgctcg acgtcggggc gcgtgtgttc
      241 gtcggcgtga acctcgacaa cgagatcgcg ccgctgaaca aggcgttcct cgacatgatg
      301 gcgggcacgc tcgcgatcgt gcgcaaggac tggcccgggc tcggcctcgt gtatcaccgg
      361 ggcatgaacg ggcgtcgcta tctcgagcag tatttccagg gcctcgtgcc gcagcgccgg
      421 cgcgacgaag gcatggacat gatgagccac ttcagcaagg agacgaccga ggagggccag
      481 ttcttccccg acaaggtggt cgccgatcac ctgatcttcc tggtgctcgc ggcgcacgac
      541 accaccacca gcgcgttgac gatggcctcc tactacctgg cgcgtcaccc cgagtggcag
      601 gagcggctgc gcggggaagt gctcggcctc gaccgcagcc tggcctacga tgacctcggc
      661 gccaatgtgc cgctgctgga gcagaccttc cacgagatcc tgcgcatgca ccctccggtg
      721 ccgcagctga tgcgccgcac catccgcgag accgagctcg gcggctaccg cattgcaccc
      781 gacacgatgg tggcgatctc gccgatcttc tcgcaccgca tgcccgaata ctggaaggat
      841 ctgcaccgct tcgacccgga gcgtttcggc cccgcgcgcc aggagcacaa gcagcatccc
      901 tacctgtggg tgcccttcgg cggcggtgcg cacaagtgca tcgggctgca ctttgccgac
      961 atgctgttca agtgcgtgat gaccgaaatg ctgcgccgtt tccgcttcag cctgccggtg
     1021 aactaccccg acccgccgtc gatcgtgcat ttcccgttcg ccaagctcac cgacgacctg
     1081 ccgttggtgc tggagccgct gggccgtgcc tgagcgcatc gcgcgcctgc tggcggcgct
     1141 ggcgttcgcg ctcggcgtcg cggccgacgt cagcgcgctg gagaccggcg aggcgctgcc
     1201 cgcgctgcgc atcgaggaac tcggcgagct gcaactggcc ggcgagacga tccgcttcgc
     1261 gccgtggcag agcggcgcgc tcacgggcaa ggtgcaggtg ctgcagtacc tggcggcgcg
     1321 catgtcggca aaatcgctga acgagccctt cacggatacc ctgcgcgatt cgggcatccc
     1381 gatcgagcgc tatcatctca ccacgatcgt gaacctcgac gatgcgctga tcggcacgcg
     1441 cggcatggtg ctgtccgagc tcgagagcaa caagaagcgc tattttcgtt cgagcatcgt
     1501 ggcggacgcg cacgggctcg ggcgcgaggc ctggcagttg ggcccgaagt cgagcgcgat
     1561 catcatcctc gcgcccgatg gatccgtgcg cttcttccgc gacggtccca tgaacagcgc
     1621 ggacatcgcg caggcgatgc gactcgtgcg ctgcggtgca gttgagcagc ttgcgctgct
     1681 ggcaccggag ttgcagtccg gctgcgatgg tgcccgatga cctcgcgata ccccacaagg
     1741 atcactgcat gagcagcatt gccctggatt tcccgcgccg cgcactgcgc aacgtcatgc
     1801 accgcatcga ggtgccacgc gacatcgggg ccgtcacgca catcgacagc gccagcgaat
     1861 gcgatccgct cgaggcgggc atcgcgcacg ggcaggccgc ggcgatctgg cgcgcggtcg
     1921 aggacctgta ccgcaccggc tactaccccg cggtgatgtt ctgcctgcgc cgcgagggca
     1981 aggtggtgtt caaccgcgcg ctcgggcatg cgcggggcaa cggccccgag gatgacgccg
     2041 ggacgccgaa gctgcccgcg accgcctcca cgccgagttg catcttctcg gcgtcgaagg
     2101 ccatcacggc gatggtgctg caccgcatgg aggagaaggg cgagatcaac ctgctcaacc
     2161 cgatcagcca ctacatcccc gagttcgcca ggcacggcaa ggaacgcacc acgatctacc
     2221 acctgctgtc gcaccgcgcc ggcataccgg gcatcgcgaa cgtcgacgac ccgcgcgtgg
     2281 tgctcgacca cgagcagagc ctgcagctgc tgtgcgaggc gcagccgcag cacctcttcg
     2341 ggcgcgagca ggcctaccac gcgatcaccg gcggtgtgat cctcgccgag atcgtgcgcc
     2401 gcgtcagcgg catggacatc cgcaaggcct ggcgcacctg gttcaaggag ccgatggggc
     2461 tgaaggtgct ggactacggg gccagcgcgc cggtgcgcgc caaaatcgcc gggcaggcgc
     2521 tgaccgggat tcagggttgc cggctggtgg acgattttgc ccgccgcgcg atcggcgcga
     2581 cctttgccga cgtgatggaa ctgctcgcca ccgccgactt ctacaaggcc gtgattccct
     2641 ccggcaacat ggtgtctacc gcggaagaag cgagcgcgtt ctaccagatg ctgctcgacg
     2701 gcgggcgctg gaacggccgc cagatcctgc ggccggagac tatccagcgc gccacgatgg
     2761 aagtcgggcc ccacgtcctc gaccgctcga tcggcatgcc gttgcgtttc agccagggtt
     2821 tcatgctggg tggcgcgccc ctgggcctgt tcggccagcg ctcgcagcac gccttcggcc
     2881 acatcgggct cgcgaacaac atcacctggg ccgaccccga gcggcagatc tcggtggcgc
     2941 tgctggtgag cggcattccg gtgctcgccg gaaactacgt tgcgctgctg aagctgatcg
     3001 cgcgcatcgg cggcgcctgc ccgcgcgtcg agaactgatg ttatgtgtgt gcgcaccgaa
     3061 cgctgcgggc gtgtcgcgac gatcgtcatc gaccggcccg cgtgccgcaa cgccgtcgac
     3121 gggcccacgg ccgccgcact ggccggggcg ttccgtgatt tcgatgccga tgattccctc
     3181 gacatcgcgg tgctgtgggg cgcgggcgga accttctgcg ccggcgccga cctgaaggcc
     3241 gtggcgcagg aacccgcgcg agccaatcgg gtggcgcccg acggcgatgg gccgatgggc
     3301 atatcgcggc tgcgcctgaa caagcccgtg atcgcggcgg tcgcgggcca cgcggtcgcc
     3361 ggcgggctcg agctcgcggc ctggtgcgac ctgcgcgtgg cggaagagag cgcagtgttc
     3421 ggggtgttct gccggcgctg gggcgtgccg ctgatcgacg gcggaacggt gcggctgccg
     3481 cggctgatcg gcatgagcca cgcgctcgac atgatcctca cggggcgcgc ggtcggcgcg
     3541 cccgaggcgc tggcgatggg gctcgcgaac cgcgtggtgc ccgacggcac ggcgcgcgaa
     3601 gctgccgagg ccctggccgc ggaaatcgcg cgcttcccgc aggcctgcct gcgcgcggat
     3661 cgcctcagcg cgctggagca ggccggtctt cccgtcgagg aggccatgcg caacgaattc
     3721 gcgcgaggcg cggcaattgc cgcgatgccc gagatgcgtg aagctgcggc gcgcttcacc
     3781 ggcggtgccg gtcgccacgg cacgtaggtc cggattca
//
LOCUS       DAORUQ010000255         3705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_32898_length_3705_cov_5.225753, whole genome shotgun sequence.
ACCESSION   DAORUQ010000255 DAORUQ010000000
VERSION     DAORUQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32898_length_3705_cov_5.225753"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1269
                     /locus_tag="PLW13_15005"
     CDS             106..1269
                     /locus_tag="PLW13_15005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY71737.1"
                     /translation="MSASSGTVRRNQGLDVLRSIAIAMVLANHAALGFFFDTGRLKWV
                     GMSAGMSSLSVLSIEWLFVLSGFLIGTMMIRSFESGRDWRGCARDFWYRRWFRTLPNY
                     YLFLAVNALLVHWGIAKGDFRWSFAFFSQNLAWAEQQPLFFGESWSLALDEWFYFLLP
                     LLIGALMLVARLPLRAMFWIASGILIAAPTAARFWLASDLDFFAWDANIRRVTVFHLD
                     ATGWGVLAAILNRWHPAWWNGHRLRKALAGVLLTALACGMIWHLAVNGWQRSPWTGAA
                     HAIMLSLPAAGLVLFLPYLCSIPLNARPLRYIAGRISLYSYSIYLVHFPALFVMCRLF
                     GVDSSIGTAKLIAAVMTWLGATIAVSAIVYHSFEGPVSDIRERFTRRVNANPF"
     gene            complement(1273..3552)
                     /locus_tag="PLW13_15010"
     CDS             complement(1273..3552)
                     /locus_tag="PLW13_15010"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY71738.1"
                     /translation="MLRAGKVPQAIELMQNHLRRNPDDVQVLMRLAGVAAELGAVPQA
                     LSALRKVVQVAPEHGHAWFGIADLEARRGNQAEADTCYRRAGYLLQSDPAVAYNHGVL
                     LEEMGRTDEAIDAYREAILRRNDFAQAHNNAGTLLLAKGETALATRHFNAALRADPGL
                     VEATFNLGLIAEGAGDRAGAETRFRAVLDRDGAHHGALLGLARQLTGGDGPQRLEALA
                     LCDGAILNQPDDPHAHHARGVVLERLRRFADAAQAFRDAWRLSAGTMHAALGDVALAL
                     HKAGRFQEALAALSELGGRRELAAGQRLTLLNLRLHACDWNSLADDVRALCQAIAAGD
                     GIGELTPALALPVPGLDEAQMHALSTGFAAALVASSVSVPAPQAHATAAAAARPLRVG
                     YLSADFREHAVSHVLAGVLERHDPQRIEAYAYSYGPDTDSPMRTRLRRTFGPRFRDIA
                     GADDAAAAATMRADGIDILVDLAGWTGNSRYEILLQDPAPVKVSWLGYAGSMGHRAFA
                     DYLIGDAIATPETSAPWFSETLALMPHSYMPSDNGRVRGARPSRSEAGLPETGVVFCC
                     FNQPYKITPDLFDVWCRLLGEVPGSVLWLVTPNADARANLLREAESRGVDASRLVFAP
                     YAPTIEEHLGRLQLADIALDTAPYNSHSTGLDALWCGVPLVCVRGQRFAGRVAASMVS
                     AAGVPELAAADLEEYRRIALRLATDSAALLGIRQRLEDARRTSALFDTAGFARDLEQL
                     YLRMHEDSAAGRRELIRLS"
BASE COUNT          585 a         1231 c         1250 g          639 t
ORIGIN      
        1 cccttttcaa cgacaccgcg caccggccat tgagcgctgc gcgcgggtgc caagagacac
       61 gaccatgcag attcacggat tccccctggc gcgggcagcc cgggcatgag cgcgagcagc
      121 ggcacggttc ggcgcaacca ggggctcgac gtgttgcgca gcatcgcgat cgccatggta
      181 ctggcgaatc acgcggcgct cgggttcttt ttcgacacgg gccgactgaa atgggtcggc
      241 atgtcggcgg gcatgagttc gctgagtgtg ctgtccatag aatggctgtt cgtgctgagc
      301 ggcttcctga tcggcacgat gatgatccgc agcttcgagt cggggcgcga ctggcggggc
      361 tgcgcccggg acttctggta ccggcgctgg ttcagaacgc tgccgaacta ttaccttttc
      421 ctggccgtca acgcactgct ggtgcactgg ggtatcgcca agggagactt ccggtggtcg
      481 ttcgcgttct tttcgcagaa cctcgcatgg gccgagcagc agccgctgtt cttcggtgaa
      541 tcgtggtcgc tcgcgctgga cgaatggttc tacttcctgc tgcccttgct gatcggcgcc
      601 ctcatgctgg tcgcgcgact gccgctgcgg gcaatgttct ggattgcatc gggcatcctg
      661 atcgccgcac ccacggcggc acgattctgg ctggcatcag acctggattt ctttgcctgg
      721 gatgcgaata tccgccgggt gacggttttt cacctggacg ccaccggctg gggggtgctg
      781 gcagccatcc tgaaccgctg gcaccccgca tggtggaacg ggcaccgatt gcgcaaggcg
      841 ctcgcgggcg tgctgctgac ggcactcgcg tgcggcatga tctggcacct ggccgtcaac
      901 ggctggcagc ggagcccgtg gacgggcgcg gcccacgcga tcatgctgag cctgccggcg
      961 gcggggctgg tgctgttcct gccctatctc tgctcgattc ccttgaacgc caggccgctg
     1021 cgctatatcg ccggcaggat cagcctgtat tcgtattcga tctacctggt gcatttcccg
     1081 gcgttgttcg tcatgtgcag gctgttcggc gtggacagct cgataggaac cgccaagctg
     1141 atcgcagccg tcatgacgtg gctaggcgcg acgatcgccg tatcggcgat cgtctatcac
     1201 agtttcgagg gtcccgtgag cgacatccgg gagcgcttca cccggcgcgt gaacgccaac
     1261 cctttctgag cctcaggaaa gcctgatgag ttcgcgcctg ccggccgcac tatcctcgtg
     1321 catgcgcagg tagagctgct cgagatcacg ggcaaacccg gccgtatcga atagcgccga
     1381 cgtacggcgc gcatcttcca ggcgctgcct gatgccaagc aacgccgcgg aatccgtggc
     1441 cagccggagt gcgatccgac gatattcctc caggtcggct gccgccaact cgggcacgcc
     1501 ggcggcgctc accatgctgg cggcgacacg accggcaaaa cgctgccccc gcacgcacac
     1561 caggggaacg ccgcaccaca gcgcatcgag ccccgtggag tgcgagttgt acggtgccgt
     1621 gtcgagtgcg atgtccgcaa gttgcagcct gccgaggtgc tcctcgatgg tgggcgcgta
     1681 aggcgcgaac accagccggg aagcgtccac gccccgggac tccgcctcgc gcagcaggtt
     1741 cgcgcgggca tccgcgttgg gcgtgaccag ccagagcacg ctgcccggaa cctcgccgag
     1801 cagtcggcac cacacatcga acagatccgg cgtgatcttg tacggctggt tgaagcaaca
     1861 gaagaccacg ccggtctcgg gcagtcccgc ctcgctgcgg gaagggcgag cgccgcgcac
     1921 acggccgttg tcgctcggca tgtagctgtg cggcatcagc gcgagcgttt cactgaacca
     1981 gggtgcggaa gtttccggcg tcgcgatcgc atcgccgatc aggtaatcgg cgaaggcgcg
     2041 gtgccccatg gatcctgcat acccgagcca gctgaccttg accggtgcgg ggtcctgcag
     2101 gaggatttcg tagcgcgaat tccccgtcca ccccgcgagg tcgaccagga tgtcgatgcc
     2161 gtcggcccgc atggttgccg ccgctgccgc atcgtccgcg cctgcgatgt cacggaaacg
     2221 cggaccgaag gtacggcgca agcgcgtgcg catcggcgaa tccgtgtcgg gcccgtagga
     2281 ataggcatat gcctcgatgc gctgcggatc gtggcgttcc agtaccccgg cgagcacgtg
     2341 gctgacggcg tgctcgcgga aatccgccga caggtagccg actcgcagcg gccgcgccgc
     2401 ggcggctgcc gttgcgtgcg cttgcggcgc cggcacggaa acgctcgagg cgactagcgc
     2461 ggcggcgaat cccgtgctca gcgcgtgcat ctgcgcttcg tcgaggcccg gcaccgggag
     2521 tgcgagcgcc ggcgtgagtt cgccgatgcc atcgccggcg gcgatcgcct ggcacaatgc
     2581 gcgcacatcg tcggcaagcg agttccagtc gcacgcgtgc aggcgcaggt tgaggagcgt
     2641 caggcgctgg cccgcggcga gctcgcggcg tccgccgagc tcgctcaacg cggccagcgc
     2701 ctcctggaac ctgcccgcct tgtgcagcgc cagcgcaacg tctccgagcg cggcgtgcat
     2761 cgtgccggcg ctcaggcgcc aggcatcgcg gaacgcctgc gccgcatcgg cgaacctgcg
     2821 caggcgctcg agcaccacgc ctcgcgcatg atgggcgtgc ggatcgtccg gctggttgag
     2881 gattgcgccg tcacacaggg cgagtgcctc gagccgctgc ggaccgtcgc ccccggtcag
     2941 ttgtcgcgcg agccccagca gggcgccatg gtgtgcgcca tcgcgatcga gcaccgcgcg
     3001 gaaacgcgtc tccgcgccgg cgcggtcacc ggcgccctcg gcaatcaggc cgaggttgaa
     3061 cgttgcctcg acaagtcccg gatcagcgcg cagtgcggcg ttgaagtgcc gcgtcgcaag
     3121 cgccgtctcg cccttcgcca gcagcagcgt gcccgcattg ttgtgcgcct gggcgaaatc
     3181 attgcgacgc aggatggcct cgcggtaggc atcgatcgcc tcgtcggtcc gtcccatttc
     3241 ctcgagcaac acgccgtggt tgtacgcgac agccgggtcc gactgcagca ggtaacccgc
     3301 acggcggtaa caggtgtcgg cctcggcctg gttgccgcgg cgcgcttcga gatctgcaat
     3361 accgaaccac gcatggccgt gctcgggcgc cacctgcacc accttgcgca gggccgacaa
     3421 ggcctgggga acggccccga gctcggcggc aacaccggcg aggcgcatca gcacctgcac
     3481 gtcatcggga ttgcgccgca ggtggttttg catgagctcg atggcctgcg gaaccttccc
     3541 ggcccggagc atcagttcga catcggcaat catcgttttc ccctgtgaga agtcgccgcg
     3601 gattatcgcc gatccggatg cgatgcgggc ggtttggaca catcaattgt cgtgattgcc
     3661 tcggggtcat cggagacggg cgcaataaaa aaccccagcc ggtta
//
LOCUS       DAORUQ010000256         3688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_33115_length_3688_cov_5.697220, whole genome shotgun sequence.
ACCESSION   DAORUQ010000256 DAORUQ010000000
VERSION     DAORUQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33115_length_3688_cov_5.697220"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLW13_15015"
     CDS             <1..359
                     /locus_tag="PLW13_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71739.1"
                     /translation="EANYRRGGRCNMHSWSSRGNWSACCYTPDHAEASCMWDKPKEIS
                     GGLYQSPGYEIVAHYSNRISPAMAVELWRDSAAHDAMVLNRGMWADNTWLAMGAAIGR
                     HYAVVWFGESPDPAGR"
     gene            complement(311..1630)
                     /locus_tag="PLW13_15020"
     CDS             complement(311..1630)
                     /locus_tag="PLW13_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71740.1"
                     /translation="MPDVLNDTCHCISLDEAALVRHFDAALVRAGGLPQLLVGREHLF
                     APYPVFANQAQWDAMQSVIGAAHEVMALPEFQAEALAASPWPEQYTLSAMMAFDFHPE
                     PAGAKLIEINTNAGGAYLAALLAESQRNCCEQLGELLAMPTSGRDAPARWFADFLHEW
                     RLARGTRPLARVLIVDDAPEQQFLYPEFLLFRELFRRNGIACDIADAAALEYAGGALR
                     HAGEAVDLVYNRCTDFALQDVAHAALAEAYRADAVVLTPSPRHHALYADKRQLVRLSD
                     DAFLARIGTSSAARHVLAAHVPKARIVTPADAEEIWAARKQYFFKPFAGYASRGAYRG
                     DRITKRVWEEIVGSGNYVAQAFAPPATRRAAPGEDAAALKFDLRCVSYGVDIRLLSAR
                     LYQGQTTNLRTVSGGLASVFLLKRGADELNPDQRPAGSGLSPNQTTA"
     gene            complement(1643..>2173)
                     /locus_tag="PLW13_15025"
     CDS             complement(1643..>2173)
                     /locus_tag="PLW13_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQY71741.1"
                     /translation="FVEAMHGLPGLVEQVLALDERIKDVAEQFVEKRHALFLGRGVQY
                     PVAMEGALKLKEISYIHAEAYAAGELKHGPLALVDEDMPVVTVAPNNELLEKLRSNLA
                     EVQARGGRLYVFADEQAGFADSDTVTVVQMPHVPAILEPVVYTVPLQLLSYHVAVMKG
                     TDVDQPRNLAKSVTVE"
     assembly_gap    2174..2273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2274..3265)
                     /gene="glmS"
                     /locus_tag="PLW13_15030"
     CDS             complement(<2274..3265)
                     /gene="glmS"
                     /locus_tag="PLW13_15030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQY71742.1"
                     /translation="MCGIVGAVAQRQVSGILVEGLRRLEYRGYDSAGVALLGTQGRIE
                     CIKRTGKVAALSESLDAAPLDGVTGIAHTRWATHGRPSEANAHPHTSNGRLALVHNGI
                     IENHVELRARLTAAGYEFRSETDTEVIVHLIDQLRQEGLSLLDAVKRAIIGLHGAFAI
                     AVIASDEPHRLIAARKGSPLVVGIGIGENFVASDQMALRQVTDRVIFLDEGDIVDVTT
                     LGVRITDATGRDVQRPIERLSEAADHADKGAFRHYMLKEIHEQPRAIANTLRGRIGED
                     HIYEQAFGVAADEVFANVAAVQIVACGTSYHSGLVARYWIEEHVGIPCAVEIAS"
     gene            complement(3278..>3688)
                     /locus_tag="PLW13_15035"
     CDS             complement(3278..>3688)
                     /locus_tag="PLW13_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HQY71743.1"
                     /translation="GPGCSVGPFARLRPGTRMAEGAKIGNFVETKKATIGARSKISHL
                     SYVGDALLGEDVNVGAGTITCNYDGVNKFQTTIGDGAFIGSNTSLVAPLEVGRNATVG
                     AGSVLTSAVPDAALAVARGKQRNIEGWKRPQKKS"
BASE COUNT          548 a         1196 c         1241 g          603 t
ORIGIN      
        1 tcgaggcgaa ctaccgtcgc ggcgggcgct gcaacatgca ttcgtggtcg agccgcggca
       61 actggtccgc gtgctgttac acgccggacc atgccgaggc cagctgcatg tgggacaagc
      121 cgaaggagat ctccggcggt ctctaccagt caccgggcta cgagatcgtg gcgcattaca
      181 gcaaccggat cagccccgcg atggcagtcg agctctggcg ggatagcgcc gcacacgacg
      241 ccatggtgct gaaccgcggc atgtgggccg acaacacctg gctcgcgatg ggtgccgcga
      301 tcggccggca ctacgcggta gtctggttcg gcgagagccc ggacccggcg ggccgttgat
      361 cgggattcaa ttcgtccgcc ccccttttca ggaggaatac ggaggcgagg ccaccggaaa
      421 cggtgcgcag gttggtggtc tggccctggt agagccgcgc gctcagcagg cggatatcga
      481 cgccgtagct gacacagcgc aggtcgaact tcagcgcggc ggcatcctcg cccggtgcgg
      541 cgcggcgggt ggccggcggc gcgaaggcct gcgcgacgta gttgcccgag ccgacgattt
      601 cctcccacac ccgcttcgtg atgcgatcgc cgcggtaggc gccgcggctg gcgtagcccg
      661 cgaagggctt gaagaaatac tgcttgcgcg cggcccagat ttcctcggcg tctgccgggg
      721 tcacgatgcg cgccttcggc acgtgcgcgg cgagcacgtg gcgtgcagcc gatgaggtgc
      781 cgatgcgggc gaggaaggcg tcgtcggaca gccgcaccag ctggcgcttg tcggcataca
      841 gcgcgtggtg gcgcggggag ggcgtgagca ccacggcgtc ggcgcgatac gcctcggcga
      901 gcgccgcgtg cgcgacatcc tgcagggcga aatccgtgca acggttgtag acgagatcga
      961 ccgcctcgcc cgcgtggcgc aacgcaccgc cggcgtattc gagcgcggcc gcatcggcga
     1021 tatcgcaggc aatgccattg cggcggaaca gttcgcggaa cagcaggaac tcggggtaga
     1081 ggaactgctg ctccggagca tcgtcgacga tgagcacgcg cgcgagcggc cgcgtgccgc
     1141 gcgccagccg ccattcgtgc aggaaatcgg cgaaccagcg cgccggcgca tcacgcccgc
     1201 tggtcggcat tgcgagcagc tcgccgagct gctcgcagca gttgcgctgg ctctcggcga
     1261 gcaacgcggc gaggtaggct ccgccggcat tggtgttgat ctcgatcagc ttggcgcccg
     1321 ccggctcggg atggaaatcg aaggccatca tcgcgctcag cgtgtactgc tcgggccatg
     1381 ggctcgcggc cagcgcctcg gcctggaact caggcagcgc catcacttcg tgcgcggcgc
     1441 cgatcaccga ctgcatcgcg tcccattgcg cctggttggc gaacacgggg tagggcgcga
     1501 acaggtgctc gcgccctacc agcagttgcg gcagtccgcc tgcgcgcacc agcgcggcgt
     1561 cgaaatgccg caccagcgcc gcttcgtcca gcgagatgca gtggcaggtg tcgttcagca
     1621 catccggcat tgcaatgccg agctactcga cggtcacgga cttcgcgaga ttgcgcggct
     1681 ggtcgacgtc ggtgcccttc atgaccgcga cgtgatacga gagcagctgc agcggcacgg
     1741 tgtagaccac gggctccagg atcgcgggca cgtgaggcat ctgcaccacg gtgacggtat
     1801 cgctgtcggc gaatccggcc tgctcgtcgg cgaacacgta gagccgtccc ccgcgcgcct
     1861 gcacctcggc gagattcgag cgcagcttct ccagcagctc gttgttgggt gccacggtca
     1921 cgaccggcat atcctcgtcg accagcgcca gcggcccgtg cttgagctcg ccggccgcgt
     1981 aggcttcggc gtgaatatag gaaatctcct tgagtttcag cgcgccctcc atggccaccg
     2041 ggtactgcac gccgcggccg aggaagagtg cgtggcgctt ctcgacgaac tgctcggcga
     2101 catccttgat gcgctcgtcc agcgcgagca cctgctcgac caggccgggc aggccgtgca
     2161 tggcttcgac gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgctcg
     2281 cgatctcgac ggcacaggga atgccgacgt gctcctcgat ccagtaacga gccaccagcc
     2341 cggagtggta actggtaccg caggccacga tctggaccgc cgcgacattg gcgaacactt
     2401 cgtcggcggc gacgccgaag gcctgctcgt agatgtggtc ctcgccgatg cgcccgcgca
     2461 gcgtgttggc aatcgcgcgc ggctgctcgt ggatctcctt cagcatgtaa tggcggaagg
     2521 cgcccttgtc ggcgtggtcg gcggcctcgc tgaggcgctc gatcgggcgc tgcacgtcgc
     2581 ggcccgtggc gtcggtgata cgcacgccca gcgtggtgac atcgacgatg tcgccttcat
     2641 cgaggaagat cacgcggtcg gtcacctggc gcaaggccat ctgatcggaa gcgacgaagt
     2701 tctcgccgat gccgatcccc accaccagcg ggctgccctt gcgcgccgcg atcaggcggt
     2761 ggggttcgtc gctggcgatc accgcgatcg cgaaggcgcc gtgcagcccg atgatggcgc
     2821 gcttcacggc gtcgagcagc gacaaccctt cctggcgcaa ctggtctatc aggtggacga
     2881 tcacctcggt gtcggtttcc gagcggaact cgtaaccggc cgccgttagg cgcgcccgca
     2941 gctctacgtg gttctcgatg atgccgttgt gcaccagcgc gagtcggccg ttgctggtgt
     3001 gcgggtgggc gttcgcttca ctggggcgcc cgtgcgtggc ccagcgcgtg tgggcgatgc
     3061 cggtcacgcc gtcgagggga gcggcgtcca ggctctcgct gagcgcggcg accttgccgg
     3121 tgcgcttgat gcattcgatg cgcccctgcg tcccgagcag cgccactccc gcggagtcgt
     3181 agccgcggta ttcgaggcgg cgcagcccct cgacgaggat gccgcttacc tgtctttgcg
     3241 ccaccgcgcc gactattccg cacatatgcc tgtttcctta cgattttttc tgcgggcgct
     3301 tccagccttc gatgttgcgc tgcttgccgc gcgccaccgc cagcgccgcg tcggggaccg
     3361 cgctggtgag cacggagccc gcacccacgg tggcgttgcg tcccacctcg agcggcgcca
     3421 cgagcgaggt gttcgagccg atgaatgcgc cgtcgccgat cgtggtctgg aacttgttca
     3481 ccccgtcgta gttgcaggtg atggtgccgg cgccgacgtt cacatcctcg ccgagcagcg
     3541 catcgcccac gtagctcagg tggctgatct tgctgcgcgc gccgatcgtt gccttcttcg
     3601 tttcgacgaa attgccgatc ttcgcaccct cggccatgcg cgtgccggga cgcaggcgcg
     3661 caaaggggcc gacgctgcag cccggacc
//
LOCUS       DAORUQ010000257         3666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_33415_length_3666_cov_5.475215, whole genome shotgun sequence.
ACCESSION   DAORUQ010000257 DAORUQ010000000
VERSION     DAORUQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33415_length_3666_cov_5.475215"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1418)
                     /locus_tag="PLW13_15040"
     CDS             complement(258..1418)
                     /locus_tag="PLW13_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71744.1"
                     /translation="MLVDFAVEDFEGERLHRELASIRARAPVSHALFAGQPAILITGH
                     QALAEAFRDNERFPPADAYRITIEPVQGVTFQTMEDEQHRLYRRLATPAFRSRAVDAM
                     EQEGLARIAHELLDRLAGTRQADLAASFTHRVAFLVISRMLGIPAHREDVFRDWAVGF
                     LEYPRDPEHSRRCAAQITDYLLPIIAQRRREPRDDVVSALAMADADGHRLTDEEILAH
                     IRLLFSAGASTTTDALGNLLYALLSERQRWERLLAAPALRGNAIEELLRWETPVAVLP
                     RLSASRAVEFFGVGIPPATFCLFAIAAANRDPAVFEHPDRFDLERDSGKKLLSFGPGP
                     RLCPGMHLARRQLAVALDVLMERLPDLHLLDVDAARPRGAILRGPARLPVAW"
     gene            1660..2358
                     /locus_tag="PLW13_15045"
     CDS             1660..2358
                     /locus_tag="PLW13_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71745.1"
                     /translation="MQDKRLAFSTVAACAAFMALGTSAWAATTVQIGSSGKTYASSAV
                     QCAADPVTGELAPVVQAGLFNPKINASATVLLNSATVATLTSADPATDVWLADGNNTV
                     VVALNRKTADTYRFTVQPGMCDLPDTSGNTFSADGTLEYAASGKSYASVTPGCAWNPA
                     TGSAQPYVHLMDNGNYLLNVSVNGEALTQLSATRPHTPVFLRAGQNVIAAANGYVSVD
                     YYVRDGGDGTCTLP"
     gene            complement(2377..3264)
                     /gene="yghU"
                     /locus_tag="PLW13_15050"
     CDS             complement(2377..3264)
                     /gene="yghU"
                     /locus_tag="PLW13_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HQY71746.1"
                     /translation="MADESAYTPPTVWTWNKDAANPFASINRPVAGATHDRELPVGRH
                     PLQLYSLATPNGIKVTVMLEELLALGHRGAEYDAWLIRILDGEQFGSGFVAVNPNSKI
                     PALLDRSTAVPTRVFESGAILLYLAEKFGAFLPGTLNERAECLSWLFWQMASTPYLGG
                     GFGHFYAYAPTKIEYAIDRFTMEVKRQLDVLDRQLAHNEYVAGAVYTIADMAIWPWYG
                     ALVKGMLYGGGEFLDVQSYSHVIRWAEAIARRPAVRRGRMVNCTWGDPAGQLHERHDA
                     SDFEHRTQDKLEPAGTAQG"
BASE COUNT          669 a         1253 c         1168 g          576 t
ORIGIN      
        1 gcgagaccgc ggcctcgaga cgttcgagcg ccggttgaac ttcatccgca agcagggcac
       61 cgggcaccgt gccgctgtgt cccatcaatt ccagcagtcg gaccgccacg tcgccgaaca
      121 tggtgatgtg cgcatacgcg gggcaggaaa aagtgaccaa catgacgact tcctggctga
      181 tggtgacatt gcgggcaatc aggccgggcc gattatgatc ccggcactga cgaagtggca
      241 aaaccggcaa tcgacgccta ccaggccacg ggcagccgag ccggaccacg caggatcgct
      301 ccccgcggcc gagcggcgtc cacgtcgagc agatgcaggt ccggaaggcg ttccatcagc
      361 acgtcgagcg caacagccag ctgacgacgg gccagatgca ttcccgggca caggcgcgga
      421 cccgggccga agctgagcag cttcttgccg ctgtcgcgct cgaggtcgaa acgatccgga
      481 tgctcgaata ccgcaggatc acgattggcc gcggcgatcg caaacaggca gaacgtcgcg
      541 ggtggaatcc cgacgccgaa aaactccacc gcccgggagg ctgacaggcg cggcagcacc
      601 gctaccggcg tctcccagcg cagcagttcc tcgatggcat tgccgcgcag cgccggcgcc
      661 gcgagcaggc gttcccaacg ctggcgctcg ctcagcagtg catacaacag gttgcccagt
      721 gcgtcggtcg tggtggacgc gccggcactg aacagcagcc ggatgtgcgc caggatctcc
      781 tcgtccgtca ggcggtgtcc gtcggcgtct gccatcgcca gcgcggaaac gacgtcgtcg
      841 cgcggttcgc gccggcgctg cgcgatgatc ggcagcaggt agtcggtgat ctgcgccgcg
      901 cagcgccgtg aatgctcggg atcgcggggg tactccagga aacccaccgc ccaatcgcga
      961 aacacatctt cccggtgagc aggaattccg agcatgcggc tgatcaccag gaatgccacg
     1021 cggtgcgtga aggatgccgc cagatcggcc tgccgcgtgc ccgccaggcg atcgagcagc
     1081 tcgtgcgcaa tgcgcgccag gccctcctgc tccatggcat ccacggcgcg cgagcgaaag
     1141 gcgggcgtgg ccagccggcg atagagtcga tgttgctcat cctccatggt ctggaaggtc
     1201 acgccctgca ccggctcgat ggtgatacgg taggcatcgg ccggcggaaa gcgctcgttg
     1261 tcgcggaatg cctcggcgag tgcctgatgc cccgtgatga gtatcgcggg ctgtccggcg
     1321 aagagcgcgt ggctcaccgg cgcccgcgcg cggatcgacg ccagctcccg gtgcaggcgc
     1381 tcgccttcaa agtcctcgac cgcgaaatca accaacatat gcgacccccg ctgcccccgg
     1441 gatcccccct cgccgcccgc gggagcaccc tggcggtgag gcgccccctg tcgccgtcaa
     1501 ttcaggttca cggtacgcgc cgatcgcccg cgcctcttaa gcttcattta aggttaccgg
     1561 aatagactga tggccactac gggatttacc acggcagctc atccgcccgg ccagccatgt
     1621 cgctggtgcc ggcacgatga catcgacagg gagcaaacca tgcaggacaa gcgactcgca
     1681 ttctcgacag tagccgcgtg tgcggccttc atggccctcg ggacatccgc ctgggcagcg
     1741 acaacagtgc aaatcggatc gagcggcaag acctacgcct ccagcgcggt gcaatgtgcc
     1801 gccgacccgg tcaccggcga attggcgccg gtggtgcagg ccggactctt caacccgaag
     1861 atcaatgcca gcgcgacggt actcctcaac agcgctaccg tggcgacgct cacgagcgcc
     1921 gacccggcca ccgatgtctg gctcgccgat ggcaacaaca cggtcgtcgt ggcgctcaac
     1981 cgcaagacgg ccgatacgta ccggttcacc gtacaacccg gcatgtgcga cctgccggac
     2041 acctccggca acaccttcag cgccgacggc acgctggagt atgccgccag cggcaagtca
     2101 tacgcctcgg tcacacccgg ctgcgcctgg aacccggcca ctggatccgc gcaaccgtat
     2161 gtccacctga tggataacgg caactacctg ctgaacgttt ccgtcaatgg cgaggcgctc
     2221 acgcagctga gcgcaacacg gccccacacg ccggtcttcc tgcgagccgg gcagaacgtc
     2281 atcgcggccg ccaacggcta cgtcagcgtc gattactatg ttcgcgacgg tggcgacgga
     2341 acctgcacgc tgccgtagcg cacgatgccg tagcgctcat ccttgcgctg tacccgcagg
     2401 ctcgagcttg tcctgcgtcc ggtgctcgaa gtcgctggcg tcgtggcgtt cgtgcagctg
     2461 gccggccgga tcgccccagg tgcagttgac catgcgcccg cgcctcaccg cgggacgccg
     2521 ggcgatggcc tcggcccagc ggatgacatg gctgtaggac tgcacgtcga ggaactcgcc
     2581 gccgccgtag agcatgccct tcacgagcgc gccgtaccac ggccagattg ccatgtcggc
     2641 aatcgtgtac accgctcccg ccacgtactc gttatgcgcc agctggcggt ccagcacgtc
     2701 gagctggcgc ttgacctcca tggtgaagcg atcgatcgca tactcgatct tcgtgggtgc
     2761 gtaggcatag aagtgaccga agccgccgcc gagatagggc gtgctcgcca tctgccagaa
     2821 gagccacgac aggcactccg cccgctcgtt cagcgtgccc ggcaggaagg cgccgaattt
     2881 ctccgccagg tacagcagga tcgcgccgga ttcgaatacc cgcgtcggca ccgcggtgct
     2941 gcgatcgagc aatgccggga tcttggagtt cgggttcacc gcgacgaatc cgctgccgaa
     3001 ctgctcgccg tcgaggatcc ggatcagcca ggcgtcgtac tccgcgcccc ggtgccccag
     3061 cgccagcagc tcctcgagca tcaccgtgac cttgatcccg ttgggcgtgg cgagcgaata
     3121 gagctgcagc gggtgccggc caacgggcag ctccctgtca tgggtagccc ccgcaacggg
     3181 ccgattgatg ctcgcgaagg gattcgccgc gtccttgttc caggtccaga ccgtcggggg
     3241 ggtgtaagcg gactcatcgg ccacgcggga tctccttttg ccagtggttc gtgcagtgaa
     3301 tgtgtcagaa gtctatggcg aaatccacgc caagcggata cctgcgtcga ggccgcgcat
     3361 gaccgtcgcc gggaacacct gatcgcacaa cgcggccggg gtcgccgtcg acccgggccc
     3421 gaagcagccc ggaccctctc ccggcgcgca acgccggcag ccacttctgt gcaattgttt
     3481 gctgaatcga aactccgccc gggcgatcat ggtctactga tgacaagccc acccggggta
     3541 tcccgggctc gattccgcgt cgttaccgaa agaggaagtg ctcgatgaag aagctttgga
     3601 tcagcctcgt ccttgccacc gtgctgccgc tgggcgggtg cgccaacatg gaccagacgc
     3661 aacatg
//
LOCUS       DAORUQ010000258         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_33862_length_3636_cov_6.983803, whole genome shotgun sequence.
ACCESSION   DAORUQ010000258 DAORUQ010000000
VERSION     DAORUQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33862_length_3636_cov_6.983803"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLW13_15055"
     CDS             complement(<1..1106)
                     /locus_tag="PLW13_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQY71747.1"
                     /translation="MKTKLLRIAGGALLALAPALALADAPTPDKGDISWMMTATILVT
                     LMAIPGLALFYGGMVRSKNVLSVLMQVMIVWCLGTVLWVVYGYSLAFTAGNAFIGGFD
                     KLFLSGVDATTLAATFSKGIYLPEYVYVVFQGSFAAITPALIVGAFAERMKFGAILLF
                     VVGWLTFAYYPIAHMVWYWDGPDAYTSADVVADVTSHAGYLFQLGALDFAGGTVVHIN
                     AGIAGLVGALLIGKRIGYGTTAMAPHNLPLVMIGASLLWVGWYGFNVGSNLESNAFAG
                     QVFLNTLLATAVATLVWTLVEWAVRGTPTVLGAASGAIAGLVAITPACGWVGPMGALI
                     IGAAAGLVCLWAVTWLKRKLGYDDSLDVFGVHCVG"
     gene            complement(1151..1489)
                     /locus_tag="PLW13_15060"
     CDS             complement(1151..1489)
                     /locus_tag="PLW13_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQY71748.1"
                     /translation="MKMVTAIVKPFKLDDVREALSDIGVQGITVSEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVPDGIVEQVIEAVTKAARTGKIGDGKIFVTPIDQAVRI
                     RTGETGEEAV"
     gene            complement(1551..2282)
                     /locus_tag="PLW13_15065"
     CDS             complement(1551..2282)
                     /locus_tag="PLW13_15065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HQY71749.1"
                     /translation="MKLKAIMAAGATALAMGSAGAAAATYSGNVAIATDYVFRGVSQT
                     QERPAIQGGFDAAFDSGFFAGIWASNVDFGTDASTEMDYYGGYTGSFGCSSCSYKVGY
                     YYYDYEGDPQLDYQELAASVTYGGLTVGLNYSWEYFGEGTTDAIGDEVEYFYPYANYT
                     YALPFWDMSLALHVGQNLMSDDGVFEPDEDQYTDWSVGLSRTFTEIGGITLGLTYYDT
                     TIDDLYGVDADDADARVVFAASKAL"
     gene            2595..2855
                     /locus_tag="PLW13_15070"
     CDS             2595..2855
                     /locus_tag="PLW13_15070"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQY71750.1"
                     /translation="MINDNLIRNLGDQLGRFIGDSAAREDMQKSLNTIVQGVFARLDL
                     VTREQFDAQLETLERTREQLTRLEDELARLQEQLAELERARE"
     gene            2894..>3636
                     /locus_tag="PLW13_15075"
     CDS             2894..>3636
                     /locus_tag="PLW13_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQY71751.1"
                     /translation="MQLATIATRAQIGIAAPAVRAEIHLSSGLPRTAIVGLPETAVRE
                     SKDRVRSAVLNSHFEFPMSRITINLAPADLPKDGGGRYDLAIALGILGASGQLNSATF
                     KGFEFIGELALNGEIRPVCGALPAALACHAAGRALILPEGNAAEAARIPGACVLPARN
                     LLQVCAHLNGSVLIDPQQPPPPTPSPPGADLAEVLGQPRAKRALAIAAAGSLNLLMCG
                     PPGTGKTMLATRMPGILPPLEDDEWLEVAS"
BASE COUNT          644 a         1229 c         1195 g          568 t
ORIGIN      
        1 ccgacgcagt gcacgccgaa gacgtcgagc gagtcgtcat agccgagctt gcgcttgagc
       61 caggtgaccg cccagagaca gaccaggcca gccgccgcgc cgatgatcag cgcgcccatc
      121 ggacccaccc agccgcaggc cggcgtgatg gcaaccaggc cggcgatcgc acccgaggcc
      181 gcgccgagca cggtcggagt gccgcgcacc gcccactcca ccagcgtcca caccagggtg
      241 gcaaccgcgg tggcgagcag ggtgttcagg aacacctggc cggcgaaggc gttggactcg
      301 aggttcgagc cgacgttgaa gccgtaccag cccacccaca gcagggaggc gccgatcatc
      361 accagtggca ggttgtgcgg cgccatcgcg gtggtgccgt agccgatgcg cttgccgatc
      421 agcagggcgc cgaccaggcc cgcgataccc gcgttgatgt gcaccaccgt gccgccggcg
      481 aagtcgagcg cgccgagctg gaacaggtag ccggcatggc tggtcacgtc ggccacgaca
      541 tcggcactgg tgtaggcgtc agggccgtcc cagtaccaca ccatgtgcgc gatcggatag
      601 taggcgaacg tcagccagcc cacgacgaac agcaggatgg cgccgaactt catgcgctcg
      661 gcgaaggcgc cgacgatgag ggcaggcgtg atcgccgcga acgagccctg gaagacgacg
      721 tacacgtact cggggaggta gatgcccttg gagaacgtgg ccgccagcgt ggtcgcgtcg
      781 actccgctca ggaacagctt gtcgaagccg ccgatgaacg cattgcccgc ggtgaaggcc
      841 aggctgtagc cgtagacgac ccacaacacg gtgccgaggc accatacgat catgacctgc
      901 atcagcacgg agagcacgtt cttcgaacgc accatgccgc cgtagaacaa cgcgaggccc
      961 ggaatcgcca tcagcgtgac caggatcgtg gcggtcatca tccacgagat gtcacccttg
     1021 tcgggggtgg gggcatcggc gagggcaagt gccggcgcga gcgcgagcaa ggcgccgccc
     1081 gcgatacgca ggagtttcgt cttcatggga tcgctccgtg tgatacctgt tggggttggg
     1141 gtgcgcgtgc tcataccgcc tcctcgcccg tttcgccggt gcggatgcgc accgcctggt
     1201 cgattggcgt cacgaagatc ttgccgtcgc cgatcttgcc ggtgcgcgcg gccttggtga
     1261 cggcctcgat cacctgctcg acgatgccgt cgggcacgac gacctcgatc ttcaccttcg
     1321 gcaggaagtc gacgacgtac tcggcgccgc gatagagctc ggtgtgcccc ttctggcggc
     1381 cgaagccctt gacctcgctg accgtgatgc cctgtacccc gatgtccgag agcgcctcgc
     1441 gcacgtcatc gagcttgaag ggcttcacaa tggcagtcac cattttcatg tttgcatctc
     1501 ctttggcgcc ccccgccgca ccgcggcggg gggctggttg tatcagccgc tcacagggcc
     1561 ttgctggccg cgaacaccac gcgcgcgtcg gcgtcgtcag catcgacgcc atagaggtcg
     1621 tcgatggtgg tgtcgtagta ggtcaggccg agggtgatgc cgccgatttc cgtgaacgtg
     1681 cggctcaggc cgaccgacca gtccgtgtac tggtcttcgt cgggctcgaa caccccgtcg
     1741 tcgctcatga ggttttgccc cacgtgcagc gcgagcgaca tatcccaaaa gggcagcgca
     1801 taggtgtaat tggcgtaggg gtagaagtac tcgacctcgt cgccgatcgc gtccgtggtg
     1861 ccctcgccga agtactccca cgaatagttc aggcccacgg tcaggccgcc gtaggtgacc
     1921 gaggccgcca gttcctggta gtcgagttgc gggtcaccct cgtagtcgta gtagtagtag
     1981 ccgaccttgt agctgcagga cgagcaaccg aagctgcccg tgtagccgcc gtagtagtcc
     2041 atctcggtgc tggcgtcggt gccgaaatcg acattcgatg cccagatgcc ggcgaagaag
     2101 ccgctgtcga aggcggcatc gaagccaccc tggatcgcgg gacgctcctg ggtctgggaa
     2161 acgccgcgga acacgtaatc ggtggctatg gccacgttgc cgctataggt ggcggcagcc
     2221 gcgcccgccg atcccatcgc cagagcggtt gcccctgctg ccatgattgc tttcagtttc
     2281 atgcttcctg tctccttgac tgcatacggt aggtattcac cgggccacac caaaacggag
     2341 tggcatcctg gctcacctcc cctactgcag acagcgtgcc aaacacacaa aatcacatca
     2401 aaacaagaag ttgaatgcag attgccacgt tcaacgactg tggcacgcca gcatccgtcg
     2461 cggcatgtcg caccaatgtg gtgcgttcga tccggcgtgg tgcgcacatc cggggtgcgc
     2521 ggcgcgtacg agcgctggca gtgcgggcgc aatcgtttag aatcgcggcc tacccgggga
     2581 gagcttgcgc ggcgatgatc aatgacaacc tgatacgcaa tctcggcgat cagctcggcc
     2641 gcttcatcgg cgacagcgcg gcgcgcgagg acatgcagaa gagcctgaac acgatcgtgc
     2701 agggcgtgtt cgcgcggctc gacttggtca cgcgcgaaca gttcgacgcg cagctcgaga
     2761 cgctcgagcg cacgcgcgag cagctcacac ggctcgagga cgagctcgcg cggctgcagg
     2821 agcagctcgc ggaactggag cgcgccaggg agtagcggcg cgcgcggatt caccatgcat
     2881 ccagggaagg atcatgcaac tcgccaccat cgccacccgt gcccagatcg gcatcgccgc
     2941 cccggcggtg cgcgccgaga tccacctctc cagcgggctg ccgcgcaccg cgatcgtggg
     3001 gctgcccgag accgccgtgc gcgagagcaa ggaccgcgtg cgcagcgcgg tgctgaactc
     3061 gcatttcgag ttcccgatgt cgcgcatcac catcaacctg gcgcccgccg acctgcccaa
     3121 ggacggcggc ggacgctatg acctggcgat cgcgctcggc atcctcggcg cctcggggca
     3181 gctgaacagc gccaccttca aaggcttcga gttcatcggc gagctggcgc tgaacggcga
     3241 gatccgcccg gtgtgcggcg cgctcccggc cgcgctcgcc tgccatgccg cgggccgcgc
     3301 gctgatcctc ccggaaggca atgccgccga ggccgcgcgc atccccggcg cctgcgtgct
     3361 gccggcacgc aacctgctgc aggtctgcgc gcacctcaac ggcagcgtgc tgatcgaccc
     3421 gcagcagccc ccgcccccga caccctcgcc gccgggcgcg gatctcgccg aggtactggg
     3481 acagccacgc gccaagcgcg cgctcgcgat cgccgcggcc ggcagcctga acctgctgat
     3541 gtgcggcccg cccggcaccg gcaagaccat gctcgccacg cgcatgcccg gcatcctgcc
     3601 gccgctggaa gacgacgagt ggctggaagt ggcgtc
//
LOCUS       DAORUQ010000259         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_34576_length_3588_cov_5.947637, whole genome shotgun sequence.
ACCESSION   DAORUQ010000259 DAORUQ010000000
VERSION     DAORUQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34576_length_3588_cov_5.947637"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PLW13_15080"
     CDS             <1..1236
                     /locus_tag="PLW13_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HQY71752.1"
                     /translation="RYGRARIGFERLIGEASADFAGTALTDAESFAESVCERHVEPYI
                     YREARRLIAEHRRLGHEIVMVTSATQFQARPVARRLGIEEVCCTQLEVKGGTLTGAIV
                     GKPCFGRGKATAGRRVARRFGVDLEHSFFYTDSLDDLPLMELVGFPVAVNAQAELAQR
                     AQREAWPQLLFSSRAVPSLEGALRTGLACNALLAAAAAGGAAWLGTRSPREARNSMMR
                     ALGDFGTGLAGLDLEVAGRDHLERVRPAVFIFNHQSMLDAVVMAQLVRHDLVAFCKQE
                     LATNPVLGPLMRASGAIFIDRGSREAARGPLRLGMAALRDGHSIAVAPEGTRGHGDDV
                     APFRHGAFLLAHKAGVPVVPVVLHNSGDAMPRGSMLLRPLQLRVTVLAPLDVSRWKAR
                     DFGPRVTQVEEQYRAVLAG"
     gene            complement(1300..2274)
                     /locus_tag="PLW13_15085"
     CDS             complement(1300..2274)
                     /locus_tag="PLW13_15085"
                     /inference="COORDINATES: protein motif:HMM:NF039286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY71753.1"
                     /translation="MSRIEPLPMRLRAPAQGFPKGWYRVADAGDVLPDALLPVSWLND
                     QFIVFRAADGQAQVADAFCPHMGAHLASHDGCLRNGRIVCPFHKWEFDAAGGALAHIP
                     YSALPPGEVGLKMHATRELDGMVLMWYHPHGSAPEFEPFETPLSRNQDGWVLYGVKEW
                     VTTCPMRDMLENLFDGPHIVYLHNSGGAPVLKSVARTPYGMRVDYDQDPSQTDAGVTG
                     FRSDFTGITLNAQIFEGTAFTTQFYNTFTPIDDERFVLHSRLHIRDSGSAEINEAIGK
                     AFTDRFMFEVEQDLKVLNYKRHLVKPRLCAGDGPIHQYRKYAAEFFVD"
     gene            complement(2271..>3588)
                     /locus_tag="PLW13_15090"
     CDS             complement(2271..>3588)
                     /locus_tag="PLW13_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71754.1"
                     /translation="SEYSRSMRAKVKTAANYGAFVGHIAIRLAAMGMDAWERAATPEE
                     IARMAAMLEDALGAGAMGLSTNFLDHDGNDRPVPTLLADDAEFSALIEVLARYPGTSL
                     QVVLDTFMRMTGPESTARLARLCAGKKVRVQWGGVPTLMFQKPIQGAMIELHEQFKRD
                     GLDFWTGFAHTGLTSVLSVNRSLIFAQSNDYVWHEVVLAETEEQKLALLRDPAWRARA
                     RESWDTQAWEHAPMKNPDRLLLENSDNNHGPVGITLADYARQLGVHHSDAMAEWLINN
                     GIQSTVQMAPFDLDEEMIVRLIKDPYSVGNINDAPAHGQMLCGAGENLELITRYWRER
                     GAITLEEAIHSMTGKLARHFNLNDRGELREGMRADIAVFHLEEIATRKKKKVYDVPDG
                     DGGFLWRWTRDAAPMRLTLVNGVPTFENGAFTEALPGEMLRPEACA"
BASE COUNT          511 a         1190 c         1277 g          610 t
ORIGIN      
        1 cggtacggcc gcgcgcgcat cggcttcgag cgcctgatcg gcgaggccag cgcggacttc
       61 gccggcaccg cgctcaccga cgccgagtcc ttcgccgaaa gcgtctgcga gcgccatgtc
      121 gagccctaca tctaccgcga ggcgcgcagg ctgatcgccg agcaccgtcg cctcggtcac
      181 gagatcgtga tggtgacgtc ggccacgcag ttccaggcgc ggccggtggc gcggcgcctg
      241 ggcatcgagg aggtgtgctg cacgcaactg gaggtgaagg gtggcacgct caccggcgcc
      301 atcgtcggca agccctgctt cgggcgcggc aaggcgacgg ccgggcggcg cgtcgcacgc
      361 cgttttggcg tggacctgga gcacagtttc ttctacaccg attcgctcga cgacctgccc
      421 ctgatggagc tggtcgggtt cccggtcgcg gtgaacgcgc aggccgaact ggcgcaacgc
      481 gcgcagcgcg aggcctggcc gcagctgcta ttcagcagtc gcgcggtgcc ttcgctggag
      541 ggagccctgc gcaccgggct ggcgtgcaac gcgttgctgg cggctgccgc ggcaggcggt
      601 gcggcatggc tgggcacgcg ctccccgcgc gaggcgcgca acagcatgat gcgcgcgctc
      661 ggcgatttcg gcaccgggct cgccgggctg gacctcgagg tggcggggcg cgatcacctc
      721 gagcgcgtgc ggcccgcggt gttcatcttc aatcaccaga gcatgctcga cgcggtggtg
      781 atggcgcagc tggtgcgtca cgacctggtc gctttctgca agcaggaact cgcgacaaac
      841 ccggtgctcg gtccgttgat gcgcgcctcg ggcgcgatct tcatcgatcg cggcagccgc
      901 gaggccgcgc gcggaccgct gcgcctcggg atggcggcgc tgcgcgacgg gcattcgatc
      961 gcggtggcac cggagggcac gcgcggccat ggcgatgacg tggcgccttt tcgccacggc
     1021 gcgttcctgc tcgcgcacaa ggccggagtg cccgtggtgc cggtggtgct gcacaacagc
     1081 ggcgacgcga tgcccagggg cagcatgctg ctgcggccgt tgcagctgcg cgtcacggtg
     1141 ctggcgccgc tcgacgtgag ccgctggaag gcgcgcgact tcgggccgcg cgtcacccag
     1201 gtcgaggagc agtaccgggc ggtgctggcg gggtagggct cagagcctgt gaatctttcg
     1261 acggcggtcg cagcaggcgg gcgaaagatt cacaggctct cagtcgacga agaactccgc
     1321 cgcgtacttg cggtactggt ggatcgggcc gtcgccggcg cacaggcgcg gtttcaccag
     1381 gtggcgcttg tagttcagca ccttcagatc ctgctccacc tcgaacatga aacggtccgt
     1441 gaaggccttg ccgatcgcct cgttgatttc tgccgatccg ctgtccctga tgtgcaggcg
     1501 cgagtgcagc acgaaacgct cgtcgtcgat cggcgtgaac gtgttgtaga actgcgtggt
     1561 gaaggcggtg ccctcgaaga tctgcgcgtt cagcgtgatg ccggtgaagt cgctgcgaaa
     1621 gccggtcacg cccgcgtccg tctgcgacgg atcctggtcg tagtccacgc gcatcccgta
     1681 gggggtgcgc gcgaccgatt tcagcacggg cgcgccgccg ctgttgtgca ggtacacgat
     1741 gtgcgggccg tcgaacaggt tctccagcat gtcgcgcatc gggcaggtcg tgacccattc
     1801 cttcacgccg tagagcaccc agccgtcctg gttgcgcgac agcggcgtct cgaagggctc
     1861 gaactcgggc gcgctgccat gcgggtggta ccacatcagc accatgccat cgagctcgcg
     1921 cgtggcgtgc atcttcagtc cgacctcgcc tggcggcagc gcggagtagg ggatgtgcgc
     1981 cagggcgccg ccggcggcat cgaattccca cttgtggaag gggcagacga tgcgcccgtt
     2041 gcgcaggcag ccgtcgtgcg aggccaggtg tgcgcccatg tgcgggcaga aggcatcggc
     2101 gacctgcgcc tggccgtcgg cagcgcggaa cacgatgaac tggtcgttca gccagctcac
     2161 cggcagcagc gcatcgggca ggacatcccc ggcgtcggcg acgcgatacc agcccttggg
     2221 aaatccctgc gctggtgcgc gcaggcgcat cggcagcggt tcgatccggc tcatgcacat
     2281 gcctccgggc gcagcatctc gccgggcagg gcctcggtga aggcgccgtt ctcgaaggtc
     2341 ggcacgccgt tgaccagcgt gaggcgcatc ggcgcggcgt cgcgggtcca gcgccacagg
     2401 aagccgccgt cgccatcggg cacgtcgtag acctttttct tcttgcgcgt ggcgatctcc
     2461 tcgagatgga ataccgctat gtcggcgcgc atgccctccc tgagctcgcc gcggtcattc
     2521 aggttgaagt gtcgcgcgag cttgccggtc atgctgtgga tcgcctcctc cagcgtaatc
     2581 gccccgcgct cgcgccagta ccgggtgatg agctccaggt tctcgccggc gccgcagagc
     2641 atctggccgt gcgcaggcgc gtcgttgatg ttgcccacgc tgtagggatc cttgatcagg
     2701 cgcacgatca tttcctcgtc gaggtcgaag ggtgccatct gcacagtgga ctggatgccg
     2761 ttgttgatca gccactcggc catcgcgtcg gagtggtgca cgccgagctg gcgcgcgtag
     2821 tcggccaacg tgatccctac cgggccgtgg ttgttgtcgg aattctcgag cagcaggcgg
     2881 tcggggttct tcatcggcgc gtgctcccac gcctgcgtgt cccaggactc gcgcgcccgc
     2941 gcgcgccagg ccggatcccg cagcagcgcg agcttctgct cctcggtttc ggccagcacc
     3001 acctcgtgcc agacatagtc gttcgactgc gcgaagatca gcgagcggtt gaccgacagc
     3061 acgctggtga gcccggtgtg cgcgaagccg gtccagaagt cgagcccgtc gcgtttgaac
     3121 tgctcgtgca gctcgatcat cgcgccctgg atgggcttct ggaacatcag cgtgggcacg
     3181 ccgccccact gaacgcgcac cttcttgccc gcgcagagcc gggcgaggcg cgccgtgctc
     3241 tcggggccgg tcatgcgcat gaaggtgtcc agcacgacct gcaggctggt gccggggtag
     3301 cgcgccagca cctcgatcag cgcgctgaat tccgcatcgt cggccagcag ggtgggcacg
     3361 gggcggtcgt tgccgtcgtg gtcgaggaag ttcgtcgaca ggcccatcgc gccggcgccg
     3421 agcgcgtcct cgagcatcgc ggccatgcgc gcgatttcct ctggcgtggc cgcgcgctcc
     3481 cacgcgtcca tgcccattgc ggcgaggcgg atcgcgatgt gcccgacgaa ggcgccgtag
     3541 ttggccgcgg tcttcacctt cgcgcgcatc gagcggctgt actcggac
//
LOCUS       DAORUQ010000260         3551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_35171_length_3551_cov_4.799485, whole genome shotgun sequence.
ACCESSION   DAORUQ010000260 DAORUQ010000000
VERSION     DAORUQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35171_length_3551_cov_4.799485"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLW13_15095"
     CDS             <1..609
                     /locus_tag="PLW13_15095"
                     /inference="COORDINATES: protein motif:HMM:NF003975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HQY71755.1"
                     /translation="WIDGRASAGTLSASVKDNLQLDDRCAVAQRLGSRILSLLGRHPL
                     FISAALPNRFFPPRFNRYAGGGHYGAHVDGALMYSDALPQAVRSDLSATLFLTDPADY
                     DGGELAIETPFGAQEVKLAAGDMVLYPSSSLHRVMPVTRGTRTSAILWLQSMVRDAAQ
                     RTLLFDLDQSIQSLSGGRDPADPDLLRLTGVYHNLLRMWAET"
     gene            650..1267
                     /locus_tag="PLW13_15100"
     CDS             650..1267
                     /locus_tag="PLW13_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY71756.1"
                     /translation="MDLWSVFQKGGPIMWPLLLTSLVSLAVVLERLFFIVREQRSRRP
                     AVIADVLLKVEQDELQDAIATGERSGDFVARTLVHGLKHHDSGFANALLQAANQELRR
                     FDQGLTVLDTAITLAPLLGLLGTVSGMIRSFGLLGSAELSAPTVITGGIAEALIATAA
                     GLGIAIVALIPFNYLNARLENARHEIEDASTRLELLLRRPERKPT"
     gene            1264..1668
                     /locus_tag="PLW13_15105"
     CDS             1264..1668
                     /locus_tag="PLW13_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY71757.1"
                     /translation="MKIPAAYQRKRARIEIIPLIDIMFFLLATFVVVSLSMIRNEGIT
                     VRLPTAQSAAQEERNASITVSISQAGEIYLDKDKVALPEMRERLGAAKRAEPELAVFI
                     NGDERADFGIAVGVLDQVRMLGIAKVSVQTVK"
     gene            1847..2917
                     /gene="ada"
                     /locus_tag="PLW13_15110"
     CDS             1847..2917
                     /gene="ada"
                     /locus_tag="PLW13_15110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HQY71758.1"
                     /translation="MATAQQRADATVADPRWAAVTARDPAADGRFVYSVRSTGVYCRP
                     SCPSRRARPENVAFHATPADAERAGFRPCRRCHPGRALPANPHARLVAGLCRLIEGAQ
                     RAPTLAELAQRAGLSPFHLHRVFKAVTGLTPAAWAKAQRARRVQHELGRADSVTAAIY
                     DAGYNSSGRFYEDAGAVLGMTPRAWRAGGVATEIRFAVGECSLGSILVAQSARGVCAI
                     LLGDDPDALLAQLQQRFPSAQLAGGDAAFESLIARVVGLVEAPQLGLELPLDIRGTAF
                     QQRVWQALRKIPPGETLSYAELARRIGAPRAVRAVAGACAANPLAVAIPCHRVVRRDG
                     GLAGYRWGIERKRALLEREAKA"
     gene            2914..>3551
                     /locus_tag="PLW13_15115"
     CDS             2914..>3551
                     /locus_tag="PLW13_15115"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HQY71759.1"
                     /translation="MKQARLAFRLALPATYRAADFLAFHRRDTAATAERVAGNTLLKG
                     MVWAGRPACLGVRFGARQAAVELLVDGADSVADADAARRMARRMLGLPQAVDDFEARY
                     GAHPELGPLIAANPGLRVPLLATPFEAMVWAVTGQQISLAAATAVRRRLIEAAGIRHS
                     DGLLCHPDPVRLACMEDAQLRAAGLSQAKAATVTLLSRGAADGTLPFEAWADA"
BASE COUNT          521 a         1252 c         1233 g          545 t
ORIGIN      
        1 tggatcgacg gccgcgccag cgccggcacg ctgtcggcat ccgtgaagga caacctgcaa
       61 ctcgacgatc gctgcgcggt ggcacagcgt ctgggcagcc ggatcctgtc gctgctgggc
      121 cgccacccgc tgttcatctc ggccgcgctg ccgaaccgct tcttcccgcc gcgcttcaac
      181 cgttacgccg gcggcgggca ctacggcgcg cacgtcgacg gcgcgctgat gtattcggac
      241 gcgctgccac aggcggtgcg cagtgacctc tccgccacgc tgttcctcac cgatccggcc
      301 gattacgacg gtggcgaact ggcgatcgag acccccttcg gcgcgcagga ggtgaagctc
      361 gccgcgggcg atatggtgct ctacccctcg agcagcctgc accgcgtgat gccggtgacg
      421 cgcggcacgc gcacctcggc catcctgtgg ctgcagagca tggtgcgcga cgccgcgcag
      481 cgcacgctgc tgttcgatct cgaccagtcg atacagtcgc tgagcggcgg gcgcgatccc
      541 gccgaccccg acctgctgcg cctcaccggc gtctatcaca acctgttgcg gatgtgggcc
      601 gaaacctgag cctcgccccg tctcgcgcca ttcaaccgag gagaaatgca tggacttgtg
      661 gagtgtgttc cagaagggcg gtccgatcat gtggcctctg cttctgacgt cgttggtctc
      721 gctggccgtg gtgctggagc gcctgttctt catcgtccgc gagcagcgca gccgccggcc
      781 cgccgtgatc gcggacgtat tgctgaaggt ggagcaggac gagctgcagg atgcgatcgc
      841 caccggcgag cgcagcggtg atttcgtcgc gcgcacgctg gtgcacggac tgaagcacca
      901 cgacagcgga ttcgcgaatg cgctgctgca agccgcgaac caggagctgc gccgcttcga
      961 ccaggggctg acggtgctgg acaccgcgat aacgctggct cccctgctgg gcctgctggg
     1021 gaccgtcagc ggcatgatcc gctccttcgg gctgctcggc agcgccgagc tctccgcccc
     1081 gaccgtgatc accggcggga tcgccgaagc actgatcgcc accgcggctg gcctcggcat
     1141 cgcgatcgtg gcgctgatcc ccttcaacta cctgaatgcc cgcctcgaga acgcgcggca
     1201 cgagatcgag gacgcctcga cccgcctcga actgctgctg cgccgcccgg agcgcaagcc
     1261 gacatgaaga tcccggcggc ataccagcgc aagcgcgcac gcatcgagat catcccgctg
     1321 atcgacatca tgttcttcct gctggcaacg tttgtcgtcg tgtctctgtc gatgatcagg
     1381 aacgagggca tcacggtgcg cctgccgacc gcgcagagcg ctgcacagga ggagcgcaac
     1441 gcatcgatca cggtctcgat cagccaagcc ggcgagatct acctcgacaa ggacaaggta
     1501 gcgctgcccg aaatgcgcga gcggctgggc gcggcgaagc gggccgagcc tgaactcgcg
     1561 gtgttcatca acggcgacga gcgtgcggac ttcggcattg ccgttggtgt cctcgaccag
     1621 gtgcgaatgc tggggatagc gaaggtctcg gtgcagacag tcaagtagtc cggtctggcg
     1681 ggtttggctt cagcccaaca ggggttgggc agggatgcaa atttccgatg tcaggctgaa
     1741 gcctgacctt ctgacaggcg aattcgaccg caagacccgg agtttcgcgc cgccgcgatg
     1801 ccgtcacact gcacgcacga tatgcttggc agcgcaggag actcccatgg caaccgcaca
     1861 gcagagagca gacgccaccg tcgccgaccc acgctgggcc gcggtgacgg cgcgcgaccc
     1921 ggccgcggac ggccgcttcg tctactcggt gcgcagcacc ggcgtgtact gtcgcccctc
     1981 gtgcccctca cgccgcgcgc ggccggaaaa cgtcgcgttc cacgcgacgc cggccgacgc
     2041 cgagcgcgcg ggcttcaggc cctgcaggcg ctgccatccc gggcgtgcgc tgccggccaa
     2101 cccgcacgcg aggctcgtgg ccgggctgtg ccgcctgatc gagggcgccc agcgcgcgcc
     2161 cacgctcgcc gagctcgcgc agcgcgccgg cctgagcccg tttcacctgc accgcgtgtt
     2221 caaggcggtg accggcctca cgcccgcggc ctgggccaag gcgcagcgtg cgcgccgcgt
     2281 gcagcatgag ctgggacgcg ccgacagtgt gaccgcggcc atctacgacg ccggctacaa
     2341 ctccagcggc cgcttctacg aggatgcggg cgccgtgctc gggatgacgc cacgcgcctg
     2401 gcgcgccggc ggtgtggcca ccgagatccg cttcgcggtg ggcgagtgct ccctcggctc
     2461 gattttggtc gcgcagagcg cgcgcggcgt gtgcgcgatc ctgctcggcg acgatccgga
     2521 tgcgctgctc gcgcaactgc agcagcggtt tccctcggcg caactggcgg gcggcgatgc
     2581 ggccttcgaa tcgctgatcg ccagggtcgt cggcttggtc gaggcgccgc aactcgggct
     2641 ggaactgccg ctggatatcc gcggcaccgc tttccagcag cgcgtgtggc aggcgctgcg
     2701 aaaaattccg cccggcgaga cgctgagcta cgccgaactc gcgcgccgca tcggcgcacc
     2761 gcgcgcggtg cgcgccgtgg ccggggcctg cgcggccaac ccgctcgcgg tggcgattcc
     2821 ctgccatcgc gtggtgcgcc gtgacggcgg tctcgccggt tatcgctggg gcatcgagcg
     2881 caagcgtgcg ctgctcgaac gcgaggcaaa ggcttgaagc aggcacggct cgcgttccgg
     2941 ctggcgctac cggcaacgta tcgcgccgcg gacttcctcg ccttccatcg ccgcgacacc
     3001 gccgcgaccg ccgaacgcgt ggccggcaac acactgctga aaggaatggt gtgggccggg
     3061 cggccggcct gcctcggggt gcgcttcggc gcgcggcagg ccgcggtgga actgctggtg
     3121 gacggagcgg actcggtggc ggatgccgac gccgcgcgac gcatggcgcg gcgcatgctc
     3181 ggcctgccgc aggccgtgga cgatttcgag gcccgttatg gcgcgcaccc cgagctcggc
     3241 ccgctgatcg cggccaatcc cggcctgcgc gtgccgctgc tggccacgcc gttcgaggcg
     3301 atggtgtggg ccgtgaccgg gcaacagatc agcttggccg cggccaccgc ggtgcgccgc
     3361 cggctcatcg aggccgccgg catccgccac tcggacggcc tgttgtgcca tcccgatccc
     3421 gtgcgcctgg cgtgcatgga ggacgcgcaa ttgcgcgcgg ccgggctgtc gcaggccaag
     3481 gcagccaccg tgaccttgct gagccgtggc gctgccgacg gcaccctgcc attcgaggcc
     3541 tgggccgacg c
//
LOCUS       DAORUQ010000261         3527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_35529_length_3527_cov_6.695565, whole genome shotgun sequence.
ACCESSION   DAORUQ010000261 DAORUQ010000000
VERSION     DAORUQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35529_length_3527_cov_6.695565"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLW13_15120"
     CDS             complement(<1..216)
                     /locus_tag="PLW13_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQY71760.1"
                     /translation="MGYPFRSRLEHDPQGDIAVIQMKDIDDANLLHAEEAIRVALPKG
                     KTHHLLRAGDLLFRSRGRSNGAAQVLDG"
     gene            complement(591..1817)
                     /locus_tag="PLW13_15125"
     CDS             complement(591..1817)
                     /locus_tag="PLW13_15125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71761.1"
                     /translation="MDRYLVISSDGHAGPRPEVYRDYLDPQYRAEFDVQHAARMAALE
                     QAGEMLEMRQETIRWGRDKAHGLAGAWDSDRRNEILDADGVAAEILFVDGLTESNSPP
                     FGGDLGLQPVGVVPELQWAGARAHNRWMAEFVAQCPLRRFGLASVTPYWGVDEAVREV
                     RWARANGLRGIMLPHMMTGQDPYHHPKYEPLWRVCAELGMVIHFHSGAAPMQEYFGAA
                     LFGPQAGAEPPAPMPGALGIYVTEVAWWLTRPLVFMIWGGVFERHPQLKVAITEGSTI
                     WVPELLALMDQRFGDHHFSAKLGTGYKQHLRLKPSEYFRRNVRVGSSAMSRREAQMRH
                     QIGLQTIMWGSDYPHPEGTWPLTRQMLVEVFGGLPETEIEAMLGGNAADFYGFDRAAL
                     APIAARIGPEKSLFRT"
     gene            complement(1840..2715)
                     /locus_tag="PLW13_15130"
     CDS             complement(1840..2715)
                     /locus_tag="PLW13_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71762.1"
                     /translation="MKELRGRVAVVTGGASGIGKALAKAFLGEGMKVVIADVEERALQ
                     AATQELGGEISGVVTDVSDPASVNALAERVFALHGACHILCNNAGVSAPNLDLWETEP
                     SDFQWVHGVNVQGVAHGVQAFVPRMIASGEEGFVMNTSSGDGGISPLAQQVVYASSKA
                     AVSIMTECLAAQLTGRGTKLRAAIFYPSGGMLDTGIWTTKRNRPAALARRQSVDPARE
                     TTFADFMEGARKAGYAMPVQDLDELAQFALAGIRNGDFVIMIGRESMEATLVERARKL
                     ARGECPIELQHMGLD"
     gene            complement(2844..>3527)
                     /locus_tag="PLW13_15135"
     CDS             complement(2844..>3527)
                     /locus_tag="PLW13_15135"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71763.1"
                     /translation="PLHIHEEIELLAMDFSTALFDTMAQLPRWTDYYLAHDQTPHYEF
                     LERCLKALQWLRGPERWVLKSPQHLEQLAPLMNAFPDARVVVTHRDPVSVMASMMTMV
                     AYAARMSRDPVRPAVIGETIRERMVLRLRALVDTRHLLAPQHSVDVLFHEFMADDVAT
                     VERIYAMADHPMTPQARGAMKRYMEEHPRGMRGQVVYDMKADFGIDPAAVREQLGFYY
                     RAFPVKVGA"
BASE COUNT          563 a         1240 c         1170 g          554 t
ORIGIN      
        1 accgtccagc acctgcgccg cgccattgct gcggccgcgc gagcggaaca gcaagtcgcc
       61 cgcgcgcagc aggtggtggg tcttgccctt gggcagcgcc accctgatgg cctcctcggc
      121 gtgcagcagg ttggcgtcgt cgatgtcctt catctggatc accgcgatat cgccctgcgg
      181 gtcgtgttcg agacgcgacc ggaaggggta acccatccgt acttctgcca gttcgcccag
      241 gctcattttt gcaaaaacgt cgttacaaca gaaaccaatt gagcgaaaca atacaggccc
      301 acccctgcac ggtcaacatg cagtaacgtc ttttctgcag aacgatgcgg cagctacgcg
      361 cccgccgctc tccagctggg cgcgatcgat ggcggcgcat ccacctccga atatgctggg
      421 tgcagcagcc ccgaagtcgg gagacgaaga cgcccggccg atggaggctg ggcgttggcc
      481 ggacagatgg tggaaaggag agtcgagcgc gcccgcggac gctcagtccg cgtgctcgta
      541 cgaacgctcg aggtcgctcg cgttgatgca gaactggtcg aggctggtat tcatgtgcga
      601 aacaggcttt tctcgggccc gatgcgcgcc gcgatcggcg ccagcgccgc gcggtcgaag
      661 ccgtagaaat ccgccgcgtt gccgccgagc atggcctcga tctcggtctc gggcaggccg
      721 ccgaacacct cgaccagcat ctggcgcgtg agcggccagg tgccctccgg gtgcgggtag
      781 tcgctgcccc acatgatcgt ctgcagaccg atctggtggc gcatctgcgc ctcgcgccgc
      841 gacatcgccg aactgccgac gcgcacgttg cgccggaagt actcgctcgg cttcaggcgc
      901 aggtgctgct tgtagccggt gccgagcttc gccgagaagt ggtggtcgcc aaagcgctgg
      961 tccattagcg cgagcagctc gggcacccag atcgtgctgc cctcggtgat cgcgaccttc
     1021 agctgcggat ggcgctcgaa cacgccgccc cagatcatga acaccagcgg gcgcgtcagc
     1081 caccacgcca cctcggtcac gtagatgccg agcgcgccgg gcatgggcgc cggcggctcg
     1141 gcgccggcct gcgggccgaa cagcgccgcg ccgaagtact cctgcatcgg cgccgcgccg
     1201 gagtggaaat ggatcaccat cccgagctcc gcgcacacgc gccacagcgg ctcgtacttc
     1261 gggtgatggt agggatcctg cccggtcatc atgtgcggca gcatgatgcc gcgcagcccg
     1321 ttcgcgcgcg cccagcgcac ctccctgacc gcctcgtcca cgccccagta aggggtgacc
     1381 gaggccagcc cgaagcgccg cagcggacac tgggcgacga actccgccat ccagcggttg
     1441 tgggcgcggg cgccggccca ttgcagctcc ggcaccacgc cgaccggctg caacccgagg
     1501 tcgccgccga agggcggcga gttcgactcg gtcagcccgt cgacgaacag gatctcggcc
     1561 gccacaccgt cggcatcgag gatctcgttg cgccggtcgc tgtcccaggc cccggccagg
     1621 ccatgggcct tgtcccggcc ccagcggatc gtctcctggc gcatctcgag catctcgccg
     1681 gcctgttcga gcgcggccat gcgcgcggcg tgttgcacgt cgaactcggc gcggtactgc
     1741 ggatcgaggt agtcgcggta gacctccggg cgcggcccgg cgtggccgtc agaagatatg
     1801 acgaggtagc ggtccatggt ctgctcccgg ggtgacggct cagtcgagcc ccatgtgctg
     1861 cagttcgatc ggacactcgc cgcgcgcgag cttgcgggcg cgctcgacca gcgtcgcctc
     1921 catcgactcg cgcccgatca tgatcacgaa gtccccgttg cggatgccgg cgagcgcgaa
     1981 ctgcgcgagc tcgtcgaggt cctgcaccgg catcgcgtag ccggccttgc gcgcgccctc
     2041 catgaagtcc gcgaaggtgg tctcgcgcgc gggatcgacg ctttgccggc gcgcgagcgc
     2101 cgccgggcgg ttgcgcttgg tggtccagat gcccgtgtcg agcatgccgc ccgaggggta
     2161 gaagatcgcc gcgcgcagct tcgtgccgcg ccccgtgagc tgcgcggcga ggcactccgt
     2221 catgatcgaa acggccgcct tgctggaggc gtagaccacc tgctgcgcca gcggcgagat
     2281 gccgccgtca ccggaggagg tgttcatcac gaagccctcc tcgccggagg cgatcatgcg
     2341 cggcacgaag gcctgcacgc cgtgcgcgac gccctgcacg ttgacgccgt gcacccactg
     2401 gaagtccgag ggctcggtct cccacaaatc gaggttgggc gccgaaacgc cggcgttgtt
     2461 gcagaggatg tggcaggcgc catgcagcgc gaacacgcgc tcggcgagcg cgttcaccga
     2521 ggccgggtcc gacacgtcgg tcaccacgcc cgagatctcg ccgccgagtt cctgcgtcgc
     2581 cgcctgcagc gcgcgttctt ccacgtcggc gatcacgact ttcatgccct cgccgaggaa
     2641 ggccttcgcg agcgccttgc cgatgccgct cgccccgccg gtcacgaccg ccacgcggcc
     2701 ccgcaattcc ttcatcgcct tgtgcctcgt caggatcggg ttgcaatgcc ggtcacgcta
     2761 tcggcatcgg cgcggcaaca caatttacag atcgctaaca atcggggcgg caaacgcacg
     2821 acgagccacg ggcctccctc ccctcaggcg ccgaccttca cgggaaaggc ccggtagtag
     2881 aacccgagct gctcgcgcac cgccgccgga tcgatgccga aatcggcttt catgtcgtag
     2941 acgacctgcc cgcgcatgcc gcgcgggtgc tcctccatgt agcgcttcat ggcgccgcgc
     3001 gcttgcgggg tcatcgggtg gtccgccatg gcatagattc gctcgaccgt cgccacgtca
     3061 tcggccatga actcgtggaa cagcacgtcg acagagtgtt gcggcgccag caggtgccgc
     3121 gtgtcgacca gcgcgcgcag gcgcagcacc atgcgttcgc ggatggtctc gccgatcacc
     3181 gcgggacgca ccggatcgcg gctcatgcgc gcggcatacg cgaccatggt catcatcgac
     3241 gccatgaccg acaccggatc gcgatgcgtc accaccaccc gcgcgtcggg gaacgcgttc
     3301 atcagcggcg ccagctgctc caggtgctgc ggcgacttca gcacccagcg ctcggggccg
     3361 cgcaaccact gcagcgcctt gaggcagcgc tcgaggaact cgtagtgcgg cgtctggtcg
     3421 tgagcgaggt aatagtccgt ccagcgcggc agctgcgcca tcgtgtcgaa cagcgccgtg
     3481 gagaaatcca tcgcgagcag ctcgatctcc tcgtggatgt gcaacgg
//
LOCUS       DAORUQ010000262         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_35849_length_3509_cov_6.030689, whole genome shotgun sequence.
ACCESSION   DAORUQ010000262 DAORUQ010000000
VERSION     DAORUQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35849_length_3509_cov_6.030689"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1530)
                     /locus_tag="PLW13_15140"
     CDS             complement(130..1530)
                     /locus_tag="PLW13_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQY71764.1"
                     /translation="MQSVVPDPNYLLVAAAAFLLGLLLGAFVLQSRGQRERSALEGLA
                     RAAQAEGAAQQARLDGALRETARREADVLRHETRAEELRQALAGARAELAQAGARHAA
                     ESRAAAEKLALLAEAREKLGLEFGAIANRLFQEKADRFSQDSKTALDLTLGPLREQLS
                     EFRRRVDEVNTGDTEGRARLLHEIAALKDLNRRMSDEALNLTRALKGDSKVQGNWGEV
                     ILERILEDSGLHKGREYETQVATVDAHGQRRMPDVIVRLPEGRDVVIDAKVSLTDYER
                     YCSAGTEPEREAALRAHVASLQAHIAALGVKDYAALEGIRTLDFVLVFVPIEAAFMRA
                     MEQDATLFGRAFERNIIMVSPTTLLATLRTIESMWRLERQNRNAEEIARRAGALHDQF
                     ARVLESLEDLGRHLERTRAAYDQTLERFARGRGNLVSRVTQLEKLGAKARKALPGTIL
                     AVADLDEDETEAQADG"
     gene            1701..2726
                     /locus_tag="PLW13_15145"
     CDS             1701..2726
                     /locus_tag="PLW13_15145"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HQY71765.1"
                     /translation="MTHASKTFVLYLGIALLGACSKTAVYTTESFANDSPFELKTIDS
                     AATACESARRSLLRQGYLIEKASAENVKGRKAYNSADNRNTFIEMNVVCVADISGSTV
                     FANGVLSTYELKKSSSAASVGLSAVGSISLPIGQSADSLVKVSEETIGDTAFYNRFFA
                     AVDRILGDMRAAAKAAEPVPEPDPEPIPEPGPAPMPATPEPATVQTIPEPATVQAAPE
                     PGPVLAAPATIPAAAPVQTAPAMTPGAVAAQPAPTTVPEPMPAGAAPTTVPEPGALPE
                     PEPEPATSPMVSEPVPAPEETTFGPAFAPATAPTAPATPESLPLPQEPDDGLEPIPLP
                     EAAPLPR"
     gene            complement(2809..2884)
                     /locus_tag="PLW13_15150"
     tRNA            complement(2809..2884)
                     /locus_tag="PLW13_15150"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2849..2851),aa:His,seq:gtg)
     gene            complement(2917..2993)
                     /locus_tag="PLW13_15155"
     tRNA            complement(2917..2993)
                     /locus_tag="PLW13_15155"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2957..2959),aa:Pro,seq:tgg)
     gene            3163..>3509
                     /locus_tag="PLW13_15160"
     CDS             3163..>3509
                     /locus_tag="PLW13_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQY71766.1"
                     /translation="MPAIVLDGKTLSQQTEAELSTRVAAIRARSGGRTPILATILVGD
                     DPASATYVRMKQNACKRVGMESTAVEMPASTTTEQLLAKIAQLNANPHCHGILLQHPV
                     PAQIDERACFDAIA"
BASE COUNT          593 a         1264 c         1123 g          529 t
ORIGIN      
        1 cttctccgga gccaccttct cggcagtctg caagctcggc gaggtcgtcg tcttccgggg
       61 ccaggttgcc gatcttcgga aatgccattg cgtgtctgct cctgtgtggg atggggtgaa
      121 tatgcgcgct cagccgtccg cttgcgcctc ggtctcgtcc tcgtcgagat ccgccacggc
      181 caggatcgtg ccgggcaggg ccttgcgtgc cttggcaccc agcttctcga gctgggtcac
      241 gcggctcacg aggttgccgc gcccgcgcgc gaagcgctcc agcgtctggt cgtaggctgc
      301 gcgggtacgt tccaggtgcc gccccagatc ttccagcgac tcgagcaccc gcgcaaactg
      361 gtcgtgcagc gcgccggcgc gccgcgcgat ttcctcggca ttgcggttct gccgctcgag
      421 gcgccacatg ctctcgatgg tgcgcagcgt cgccagcagc gtggtcgggc tcaccatgat
      481 gatgttgcgc tcgaaggcgc gcccgaacag cgtggcgtcc tgctccatcg ccctcatgaa
      541 cgccgcctcg atgggcacga acaccagcac gaaatcgagc gtgcggatac cctcgagcgc
      601 ggcgtaatcc ttgacgccga gtgccgcgat gtgggcctgc agcgaggcga cgtgcgcgcg
      661 cagcgccgcc tcgcgctcgg gctcggtccc ggccgagcag tagcgctcgt aatcggtcag
      721 cgagaccttg gcgtcgatca ccacgtcgcg cccctcgggc aggcgcacga tcacgtccgg
      781 catgcgccgc tggccgtgcg cgtccacggt ggcaacctgc gtctcgtact cgcggccctt
      841 gtgcagcccg gagtcttcca ggatgcgctc gaggatcacc tcgccccagt tgccctgcac
      901 cttgctgtcg cccttcagcg cacgcgtcag gttcagcgcc tcgtcgctca tgcgccggtt
      961 cagatccttc agcgcagcga tctcgtgcag cagccgcgcg cggccctcgg tgtcgccggt
     1021 gtttacctcg tcgacgcggc ggcggaattc ggacagctgc tctcgcagcg gacccagcgt
     1081 gagatcgagc gcggtcttgc tgtcctggct gaagcggtcg gccttctcct ggaacaggcg
     1141 gttggcaatg gcgccgaact ccaggcccag cttctcgcgc gcctcggcga gcagggccag
     1201 cttctccgcc gccgcgcgcg attccgcggc gtgccgggcg cccgcctgcg cgagctccgc
     1261 gcgcgcgccc gccagcgcct ggcgcagttc ctcggcgcgc gtctcgtgac ggagcacatc
     1321 ggcctcgcgt cgcgccgtct cgcgcagcgc cccatcgagt cgcgcctgct gcgccgcacc
     1381 ctcggcctgg gcggcgcgcg cgagcccctc cagcgccgag cgctcgcgct gcccgcgcga
     1441 ctgcagcacg aacgcaccca gcaacaagcc gagcagaaac gccgccgcgg cgacgagcag
     1501 gtaattcggg tcgggaacaa cggattgcac tgtgcgtccg tgatggcggt ggtaataggc
     1561 cgtctttacg agccggtcat gacgctttca ttataggcac cgccactacc gccactgcgg
     1621 ggcgggaata agtagtatca tttgttgcca ggaaatttgc aggcatatca ggcacatccg
     1681 gcgcggaacc acgaattttt atgacgcatg catcaaagac cttcgtcctt tacctgggca
     1741 tcgcgctgct gggcgcctgc agcaagacgg ccgtctacac cacggagagc ttcgccaacg
     1801 acagcccctt cgaactcaag accatcgaca gcgcagccac cgcctgcgag agcgcgcgcc
     1861 gctccctgct gcgccagggt tacctcatcg agaaagccag cgcggaaaac gtcaaggggc
     1921 gcaaggctta caacagcgcg gacaaccgca acaccttcat cgagatgaac gtggtctgcg
     1981 tcgcggatat cagcggcagc acggtgttcg ccaacggtgt gctcagcacc tacgagctca
     2041 agaagagcag cagcgccgcc agcgtcgggt tgagcgccgt gggctcgatc tcgctgccca
     2101 tcggccagag tgccgactca ctggtgaagg tctcggaaga gaccatcggc gacacggcct
     2161 tctacaaccg cttcttcgcg gcggtggatc gcatcctcgg cgacatgcgc gccgcggcca
     2221 aggccgcaga gcccgttccc gagcccgacc cggaacccat tccggagcca ggccccgccc
     2281 cgatgccggc aacgccggaa ccggccaccg tgcagacaat tccggaaccg gccaccgtgc
     2341 aggcggcgcc cgaaccaggg ccggttctgg cggcaccggc gacaataccg gcagccgcgc
     2401 cggtgcagac cgcaccggca atgacgcccg gggcggttgc ggcgcaaccc gcgccgacga
     2461 ccgtgcccga accgatgccc gcaggcgccg ctcccaccac ggtgccggag cccggcgcgc
     2521 tgcccgagcc cgagcccgag cccgcgacaa gcccgatggt gtccgaaccg gtcccggcac
     2581 ccgaggagac cacgttcggt cccgcattcg caccggccac ggccccgact gcgccggcaa
     2641 cgccggagtc gctcccgctg ccgcaggaac ccgatgacgg gctggaaccg atccccttgc
     2701 ccgaggcagc cccgctgcct cgttgaccgg gtgagcagcg agcgcgcacg gccacgccgc
     2761 aggtgccgca ggtcgcggaa atagtctgca cgggaacgga aagatgaatg gggtggccga
     2821 cggggatcga acccgcgaca acaggaatca caatcctgta ctctaccaac tgagctacgg
     2881 ccaccatcga aaacgtcgcg ccgggtcacg gcggattggt cggggtagag ggattcgaac
     2941 ccccgacatc ctgctcccaa agcaggcgcg ctaccagact gcgctatacc ccgaagcccc
     3001 gggaccgcgg ggtcccgcca cgggagcgcg aatgttaatg gcgaccctgt cgagcgtcaa
     3061 tcgcagcatg gcgtgaattt ggcccttttg cttggccctg ccatgagccg atgcgaaaat
     3121 gcgcggctcc cgtttcaccc accgccagcg gaataccagt caatgcctgc gatcgtcctc
     3181 gacggaaaga ccctgtccca gcaaaccgaa gccgaactga gcacgcgtgt cgccgcgatc
     3241 cgcgcgcgca gcggcgggcg aacgccgatc ctggcgacga tcctggtcgg cgacgatccg
     3301 gcatccgcga cctatgtgcg catgaagcag aacgcctgca aacgcgtcgg catggaatcg
     3361 acggccgtcg agatgcccgc gagcaccacc acggaacagc tgctggcgaa gatcgcgcaa
     3421 ctgaatgcca acccgcactg ccacggcatc ctgctgcagc acccggtgcc ggcgcagatc
     3481 gacgagcgtg cctgcttcga cgcgatcgc
//
LOCUS       DAORUQ010000263         3504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_35935_length_3504_cov_6.108437, whole genome shotgun sequence.
ACCESSION   DAORUQ010000263 DAORUQ010000000
VERSION     DAORUQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35935_length_3504_cov_6.108437"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="PLW13_15165"
     CDS             complement(<1..1860)
                     /locus_tag="PLW13_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY71767.1"
                     /translation="MNFTDTFIRRPVLATVVSLLIVFLGLRAFDSLSLREYPEVTYPV
                     INVTTVYPGANAELIQGFITRPLERAIAKAEGIDYIVAHSEDGLSRISAHLQPGFDVD
                     AAFADISGKVEEERRTLPRDAEAPVVQKETGAGGNVLMFLSFSSEVLTPQQITDYLDR
                     VVQPLLSTVKGVGEARIDGGSTFAMRVWLDPARLAAFGLSAADVEQAVARNNVQSAAG
                     NIRSDYVQFYLNPQTVIRDAEGFRRLVLKRDGDSVVRLGDVARVEMGAENYDSRVSHD
                     GRVAVFVGINAAITANPLEVGRNVHAMMPDIVSQLPEGMRGEVVFDGTKTIRESIDEV
                     VKTLVEASLIVILIIFLFLGSLRSVIVPVVTIPLSLIGVLSLMLWMGFSVNLLTLLAM
                     VLAIGLVVDDAIVVVENIQRHIEEGLEPQAAALVGAREIAFPVIVMTLTLAAVYAPIG
                     FLGGLTGKLFIEFAFTLAGAVIVSGVVALTLSPMMCSRLLRAESSSSPFVALLDRNFE
                     ALKLRYRRLLERTLANRPVVLVFAAAIFVSLFFLFRAVPQELAPQEDTGHVYVVGSGP
                     LNSTIEHTLAFVEQVEQIVEQLPETAQHFFVNGAMGTNTFFGMVELEPFEERER"
     gene            complement(1857..2957)
                     /locus_tag="PLW13_15170"
     CDS             complement(1857..2957)
                     /locus_tag="PLW13_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY71768.1"
                     /translation="MKRPMALMIVALVLVFGAIFGVIEGKQILISRYLASYEPPPVTV
                     SAEEARNESWDVVIGAVGTLKAVQGIDIATEASGIVTKLHFKPGDDVRAGQELVSLDD
                     RVEVATLKSLQAQRRLADINFERDQRLIATKAISRTDFDKTEALLKDISAQVEKTEAV
                     IARKHLRAPFDGRIGIPQVEPGQYATEGQAIVTLQALDTLEVDFALPEQELPRLAVGQ
                     RVRCRVQAYPEQIFEGHVSAIDARVDGNTRNVLVRARVPNTASTLLPGMFVSVEVIVG
                     DAAEKVTVPETAVSYNLYGDSVFVVAERAREDGGKELAVTRHYVQLGPRRNGRVAVTE
                     GVAAGELVVTAGALKLDAGTVVTIDNSVKLSR"
     gene            complement(3072..>3504)
                     /gene="bcp"
                     /locus_tag="PLW13_15175"
     CDS             complement(3072..>3504)
                     /gene="bcp"
                     /locus_tag="PLW13_15175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQY71769.1"
                     /translation="LKNQDGKTVSLKDFKGKSTVVLYFYPAAMTPGCTVQACGLRDTA
                     SALAALDAVALGVSPDPEKKLHKFIERDRLNFDLLSDPDHEVALKYGAWGPKKFMGRS
                     YDGILRTTFIIGKDGRLLHVMDKVKTKSHHDDVLALLRTLD"
BASE COUNT          592 a         1204 c         1126 g          582 t
ORIGIN      
        1 gcgctcgcgc tcctcgaacg gttcgagctc gaccatgccg aagaaggtgt tggtacccat
       61 cgcaccgttg acgaagaaat gctgggcggt ttcgggaagc tgctcgacga tctgctcgac
      121 ctgctccacg aaggccagcg tgtgctcgat cgtgctgttc agcggacccg agccgacgac
      181 atacacgtgc cccgtgtcct cctgcggcgc cagctcctgc ggcacggcac ggaacaggaa
      241 aaacaggctg acgaagatgg ccgccgcgaa caccagcacc acgggccggt tcgcgagcgt
      301 gcgctcgagc aggcgccggt agcgcagctt cagcgcctcg aagttgcggt ccagcagcgc
      361 cacgaaaggc gacgacgagc tctcggcgcg caacagccgg ctgcacatca tcggcgacag
      421 tgtcagtgcc accacgcccg agacgatcac cgcgccggcc agcgtgaagg cgaactcgat
      481 gaagagcttc ccggtgagcc cgccgaggaa tccgatgggg gcatacaccg cggccagcgt
      541 cagcgtcatc acgatgaccg ggaacgcgat ctcgcgcgcc ccgaccagcg ccgctgcctg
      601 cggctccagc ccctcctcga tatggcgctg gatgttctcg accaccacga tcgcgtcatc
      661 gacgaccagc ccgatcgcca gcaccatcgc gagcagcgtg agcaggttca ccgagaaccc
      721 catccacagc atcagcgaga gcacgccgat cagcgacagg ggtatcgtca ccacgggcac
      781 gatcaccgag cgcagcgaac cgaggaacag gaagatgatc aggatcacga tcaggctggc
      841 ctcgaccagc gtcttgacga cctcgtcgat cgattcccgg atggtcttgg tgccgtcgaa
      901 aaccacctcg ccgcgcatgc cctcgggcag ctggctcacg atgtccggca tcatcgcgtg
      961 cacgttgcgc cccacctcga gcgggttcgc cgtgatcgcg gcgttgatgc cgacgaagac
     1021 cgcgacgcga ccgtcgtggc tcacccttga gtcgtagttc tccgcgccca tctcgacgcg
     1081 cgccacgtcg cccaggcgta ccaccgagtc gccgtcacgc ttgagcacca gccggcggaa
     1141 tccctcggca tcgcggatca cggtctgcgg gttgaggtag aactgcacgt agtcgctgcg
     1201 gatgttgccc gccgcgctct gcacgttgtt gcgcgcgacg gcctgttcca cgtcggcggc
     1261 cgagagcccg aaggccgcaa ggcgcgcggg gtcgagccac acgcgcatcg cgaatgtgct
     1321 gccgccgtcg atgcgggcct cgcccacgcc cttgaccgtg ctcagcaagg gctgcaccac
     1381 gcggtcgagg taatcggtga tctgctgcgg cgtcagcacc tcgctcgaga acgagaggaa
     1441 catcagcacg ttgccgccgg cgccggtttc cttctgcacc acgggggcct cggcatcgcg
     1501 cggcagcgtg cgccgctcct cctcgacctt gccgctgatg tcggcgaacg cggcatcgac
     1561 gtcgaagccc ggctgcaggt gcgcagaaat gcgcgacagg ccgtcctcgc tgtgcgcgac
     1621 gatgtagtcg atgccctctg ccttggcgat cgcccgctcc agcgggcgcg tgatgaagcc
     1681 ctggatcagc tctgcgttcg cgccgggata gaccgtggtc acgttgatca ccgggtaggt
     1741 gacctcgggg tattccctca ggctcaggct gtcgaaggcg cgcaggccca ggaacacgat
     1801 cagcaggctg acgacggtgg ccagcacggg ccggcggatg aaggtgtcgg tgaaattcat
     1861 cgcgagagct tgacgctgtt gtcgatggtg acgacggtgc cggcgtcgag cttcagtgcc
     1921 ccggccgtca ccaccagttc accagcggcg acgccttcgg tcacggcgac ccgcccgttg
     1981 cgccgcggtc ccagctgcac gtagtggcgc gtgacggcaa gctccttgcc cccgtcctcg
     2041 cgcgcacgct cggccacgac gaataccgag tcgccgtata ggttgtagct gaccgcggtc
     2101 tcgggcaccg taaccttctc ggcggcatcg cccacgatga cctcgacact cacgaacatc
     2161 cccggcagca gggtgctcgc ggtgttgggc acgcgcgcgc gcaccagcac gttgcgcgtg
     2221 ttgccgtcga ccctggcatc gatggcgctg acgtggccct cgaaaatctg ctccggatac
     2281 gcctgcacgc ggcagcgcac gcgctgcccc acggcgagcc ggggcagttc ctgttccggc
     2341 agcgcaaaat ctacctccag cgtgtccagc gcctgcagcg tcacgatcgc ctgcccctcg
     2401 gtggcgtact gccccggttc gacctgcgga atgccgatgc gtccgtcgaa gggtgcgcgc
     2461 aggtgcttgc gcgcgatcac cgcctcggtc ttctcgacct gcgcgctgat gtccttcagc
     2521 agcgcctcgg tcttgtcgaa gtcggtgcgc gagatggcct tggtggcgat caggcgctgg
     2581 tcgcgctcaa agttgatgtc ggcgaggcgg cgctgtgcct gcaggctctt cagggtggcg
     2641 acctcgacac gatcatcgag gctgaccagt tcctgcccgg cacgcacgtc gtcgccgggc
     2701 ttgaaatgca gcttcgtgac gatgcccgaa gcctcggtcg cgatatcgat gccctggacc
     2761 gccttcaacg tgccgaccgc gccgatcacc acgtcccagc tctcgttgcg ggcctcctcg
     2821 gccgagaccg tgaccggcgg gggctcgtaa ctggcgagat agcgcgagat caggatctgc
     2881 ttgccctcga tgacgccgaa aatcgcgccg aacaccagca ccagcgcgac gatcatcagg
     2941 gccatcggcc ttttcatcgg gaatatccat agaagtgaat gacgcgcgcc gcaacccgca
     3001 gcgcggcgcc attctacgga tcaaggcgct ccgaggccat gcagaccgcc ccggcttacg
     3061 caagggtaat atcaatcgag cgtgcgcagc agcgcgagca cgtcgtcgtg atgggacttg
     3121 gtcttcacct tgtccatcac atgcagcagg cggccgtcct tgccgatgat gaacgtggtg
     3181 cgcaggatgc cgtcgtagct gcggcccatg aacttcttcg ggccccacgc gccatacttc
     3241 agcgccactt cgtggtccgg gtccgagagc agatcgaagt tcaagcggtc gcgctcgatg
     3301 aacttgtgca gcttcttctc gggatcgggg ctcacgccga gcgccacggc gtcgagcgcc
     3361 gccagcgcgc tcgcggtgtc gcgcaggccg caggcctgca cggtgcagcc gggcgtcatc
     3421 gcggccgggt agaaatacag gaccacggtg ctcttgccct tgaagtcctt caggctgacg
     3481 gtcttgccgt cctggttctt cagg
//
LOCUS       DAORUQ010000264         3489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_36169_length_3489_cov_5.146476, whole genome shotgun sequence.
ACCESSION   DAORUQ010000264 DAORUQ010000000
VERSION     DAORUQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36169_length_3489_cov_5.146476"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PLW13_15180"
     CDS             <1..1180
                     /locus_tag="PLW13_15180"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQY71770.1"
                     /translation="GKTTTSRMITRIMKFAGRRVGTVCSGGFYIGEQLEGTPRQFGGD
                     LQHHLFERPDVDFAVLEEYFGRIARAGFPFRWCDVSVLTNVTADHLGRIGTHTLAEMA
                     ALKGAVATRARERVVLNADDAHCLAMAAGLPPERVCLVSQRMSHAELQARLPDARGAC
                     VLETIEDAEWIVLHDRGVRTPVIAVNDIPASFRGDARHNVSNAMQAAAACHASGAETQ
                     ALRAGLGSFVMDFDSNPGRLNIYDGLPFRVIMDYAHNADGYRTLSAFVDRQRVTGRRI
                     VMMSAVGDRLDHDVMAAAAEMAGHFDHYVCRNYLGIRGERLPQAMPELLKTGLCRAGV
                     PQDAVTTIADATEAIHFCLDMAAQGDLLVLLPGDNEFASTWELLGTFAAQLAHRETGA
                     "
     gene            1356..2198
                     /locus_tag="PLW13_15185"
     CDS             1356..2198
                     /locus_tag="PLW13_15185"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQY71771.1"
                     /translation="MLQAVAGSLSYRTIWLSDIHLGDRHCKADYLLEFLGAIRCETLF
                     LVGDIVDLWSLSNRWHWPAQHSAVLRRVMELADGGTRVVYVPGNHDEPIRDFVGNAFG
                     KIAIERQAVHLSASGKRLLITHGDEFESWIRCSAFTRFLGCASYEALLRLNRAYNRWR
                     SWRGKPYWSLATYVKHRVANARRAIDSFEQAASAHAREHDFDGIVCGHIHHPSQREID
                     GILYCNDGDWVENCTALAETAEGALELLHWPRLRARSPGLRAANDHEAATATAAAAAA
                     AATR"
     gene            2208..>3489
                     /locus_tag="PLW13_15190"
     CDS             2208..>3489
                     /locus_tag="PLW13_15190"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY71772.1"
                     /translation="MFTVEDVVCSRYPGFARQHRWLRSPVLAILRLLFHESRFRQFAQ
                     DYPHLRGLDFVDQVLEYFDFSYAVRSNERERIPAAGPLVIVANHPIGSLDGLALLRLV
                     SEIRPDVKAVANELLMAIEPLHPLLLPVDAMNGRTARDNIRRIEAHLGGGGALVIFPA
                     GEVSRLSPRGVRDGVWRSGFLRMARRARADIVPVLVDGRNSPFFYSVSMLAKPLSTLL
                     LIREMFRHASNSVAVRIGHAVSPAQYQAAASDDERLAARFRNQVYALARPQRKPLFPP
                     AAKAIAHPESRQLLRLEMRGAEALGATRDGKRIGLCRHETGSVVLREIGRLREIAFRA
                     VGEGSGERRDVDRFDPHYEHIVLWDDNDLEIVGAYRLAHAGRTIAARGGAGLYTHSLF
                     EFTPAMEAIWPEAVELGRSFVQPRYWGRRGLDYLW"
BASE COUNT          548 a         1217 c         1169 g          555 t
ORIGIN      
        1 cggcaagacc acgacctcgc gcatgatcac caggatcatg aagtttgccg gccggcgcgt
       61 gggcacggtc tgctccggtg gtttttacat cggcgagcag ctcgaaggca caccgcgcca
      121 gttcggcggc gatctgcagc atcacctgtt cgagcgcccg gacgtggatt tcgcggtgct
      181 cgaggagtac ttcggacgta tcgcccgcgc gggatttccg ttccgctggt gcgatgtctc
      241 cgtcctgacg aacgtcaccg ccgatcacct cggccgcatc ggcacgcaca cgctcgcgga
      301 gatggcggcg ttgaagggcg cggtggcgac acgcgcccgc gagcgggtgg tcctgaacgc
      361 cgacgacgcg cactgcctgg ccatggctgc cggcctcccg cccgagcgcg tctgcctggt
      421 ctcgcagcgg atgagccatg cggagttgca ggcgcggctg cccgatgcgc gcggcgcctg
      481 cgtgctggaa acgatcgagg acgccgagtg gatcgtgctg cacgatcgcg gcgtgcgcac
      541 gccggtgatc gcggtgaacg acattcccgc cagtttccgc ggcgacgccc gccacaacgt
      601 gagcaacgcg atgcaggcgg ccgcggcctg ccacgcatcc ggggcagaga cgcaggcgct
      661 gcgcgcggga ctcggctcct tcgtcatgga tttcgactcc aatcccgggc gcctgaacat
      721 ctacgacggc ctgccgttcc gcgtcatcat ggattacgcg cacaacgccg acggctaccg
      781 cacgctgagc gcattcgtgg accgccagcg cgtcacgggg cgcaggatcg tcatgatgtc
      841 ggcggtcggc gaccggctcg atcacgacgt catggcggct gcggccgaga tggcgggcca
      901 cttcgaccac tacgtctgtc gcaactacct cgggatacgc ggtgaacgcc tgccgcaggc
      961 catgccggag ctgctcaaga cgggcctgtg cagggccggc gtgccgcagg atgccgtcac
     1021 cacgatcgcg gacgcgacgg aagcgatcca tttctgcctg gacatggccg cgcaaggaga
     1081 cctgctggtg ctgttgccgg gggacaacga attcgcatcg acctgggaac tgctgggaac
     1141 attcgccgcg cagctcgcgc atcgcgaaac cggtgcctga ggcaccggtt cgctggcgcg
     1201 cgcccatgcc gccctcgcgc agcccgcgcc aagacacctc catgcccacg ggcacccacg
     1261 ccggctcgat gcccctgttc ataaatccgt agcctccctg caaacggctt gtcacctgcg
     1321 ccgccgatca tgcgcaaaag ccacgggaga gcaagatgct gcaggcagtc gccggttccc
     1381 tgagttaccg cacgatctgg ctttcggata tccacctcgg cgaccgccac tgcaaggcgg
     1441 actacctgct cgaattcctc ggcgcgatcc gctgcgagac cctgttcctg gtcggcgaca
     1501 ttgtcgacct gtggtcgctg agcaaccgct ggcactggcc cgcgcagcac agcgcggtgc
     1561 tgcgccgcgt gatggaactg gctgacggcg gcacgcgggt cgtgtacgtg cccgggaatc
     1621 acgatgaacc gatccgtgac ttcgtgggca acgcattcgg gaagatcgcg atcgaaaggc
     1681 aggccgtgca cctcagcgcc tcgggcaagc gcctgctgat cacgcacggc gacgagttcg
     1741 agagctggat ccgctgcagc gccttcaccc gctttctcgg ctgcgcctcg tacgaggcgc
     1801 tgctgcgcct gaaccgcgcc tacaaccgct ggcgatcctg gcgcggcaag ccctactggt
     1861 cactggcgac ctacgtgaag catcgcgtcg ccaacgcgcg ccgtgcgatc gattccttcg
     1921 agcaggcggc gagcgcgcac gcgcgcgagc acgacttcga cggcatcgtg tgcggacaca
     1981 tccaccaccc gagccagcgc gagatcgacg gcatcctcta ctgcaacgac ggcgactggg
     2041 tcgagaactg cacggcactg gccgaaaccg ccgagggcgc gctggagttg ctgcactggc
     2101 cgcgcctgcg cgcgcgcagc ccgggattgc gcgccgctaa cgatcacgag gccgcaaccg
     2161 caaccgctgc cgctgccgct gccgccgcaa cccgctagga gatagccatg ttcaccgtcg
     2221 aagacgtcgt ctgctcgcgc tatcccggtt tcgcccgaca gcatcgctgg ctgcgctcgc
     2281 ccgtgctcgc gatcctgcgc ctgctgttcc acgagagccg ttttcgccag ttcgcgcagg
     2341 actatccgca cctgcggggc ctcgacttcg tggaccaggt gctggagtac ttcgacttca
     2401 gctatgccgt gcgcagcaac gagcgcgagc gcatccctgc cgcgggcccg ctggtcatcg
     2461 tggcgaatca cccgatcggc agcctggacg gactggcgct gctcaggctg gtgagcgaga
     2521 tcaggcccga cgtgaaggcc gtcgccaacg agctgctgat ggccatcgaa ccgctgcacc
     2581 cgctgctgct gcccgtcgat gcgatgaacg ggcgcaccgc gcgcgacaat atccgccgca
     2641 tcgaggcgca cctgggtggc ggcggcgcgc tggtgatatt tcccgccggc gaagtctcgc
     2701 ggctcagccc ccggggagtg cgcgacggcg tatggcgcag cggcttcctg cgcatggcgc
     2761 gacgcgcgcg cgccgacatc gtcccggtgc tcgtcgacgg gcgcaactcg ccgtttttct
     2821 acagcgtgtc aatgctggcg aagccgctgt cgacgctgct tctgatccgc gaaatgttcc
     2881 gtcacgcgag caacagcgtg gccgtgcgta tcgggcacgc ggtctccccg gcgcagtacc
     2941 aggccgcggc gagtgacgac gagcgcctcg cggcgcgctt tcgcaaccag gtctacgcgc
     3001 tcgccaggcc ccagcgcaaa ccgctgttcc cgcccgctgc aaaggcgatc gcgcatcccg
     3061 agagccgcca gctgctgcgt ctcgagatgc gcggtgccga ggcgctcggt gcgacgcgcg
     3121 acggcaagcg catcggactc tgccgtcacg agaccggctc ggtggtgctg cgcgagatcg
     3181 gtcgcctgcg cgagatcgcg ttccgtgccg tcggtgaggg cagtggcgag cgccgcgacg
     3241 tggatcgctt cgatccgcac tacgagcaca tcgtgctgtg ggacgacaat gatctcgaga
     3301 tcgtgggagc ctaccggctg gcgcacgccg ggcgcaccat tgccgcgcgc ggcggcgccg
     3361 ggctgtacac ccattcgctg ttcgagttca cgccggccat ggaggcgatc tggcccgagg
     3421 ccgtcgagct ggggcgcagc ttcgtgcagc cgcgctactg gggacgccgc ggactggact
     3481 acctgtggt
//
LOCUS       DAORUQ010000265         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_36275_length_3482_cov_5.491684, whole genome shotgun sequence.
ACCESSION   DAORUQ010000265 DAORUQ010000000
VERSION     DAORUQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36275_length_3482_cov_5.491684"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..778)
                     /locus_tag="PLW13_15195"
     CDS             complement(455..778)
                     /locus_tag="PLW13_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQY71773.1"
                     /translation="MTFVVGENCINCKHTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVDAIFSEDELPADQQVFLELNAELAQVWPNITEMKEKLADAEKWNGVPDKL
                     QHLQR"
     gene            complement(877..3429)
                     /gene="mutS"
                     /locus_tag="PLW13_15200"
     CDS             complement(877..3429)
                     /gene="mutS"
                     /locus_tag="PLW13_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQY71774.1"
                     /translation="MMQQYLRIKSRHPNELLFYRMGDFYELFHDDAKRAAALLDITLT
                     SRGASAGVPIPMAGVPYHSVEGYLARLVRAGVSVAICEQIGDPATSKGPVEREVTRIV
                     TPGTLTDESLLDAARDNLLVAVAESGSRFGISSLDLSSGRFQVLEVDGEEALAAELRR
                     LQPAELLAAEGTARRPALASINGLRTRAPWEFEVRSATELLNAQFGTHDLVGFGCAQM
                     ELGIAAAGCLLRYARETQRGELPHVSALVPDSREDAVALDAASLRNLEIDTNFTGGDE
                     YTLAWVLDTTKTAMGSRWLRRWLRRPLRRLAELEARQGAIAELVDDARYLAPREALAA
                     VGDIERILARVALRSARPRDLSRLGAALESLPVLHAACAALEAPLSRQLLAAVSEFPQ
                     LATMLKCALVENPPVVVREGGVIAPGYDAELDELRLLSTGAHDFLVALEARERERTGL
                     PNLHVGYNRVHGYYIEISRAQSVNAPADYIRRQTLKNAERYITPELKAFEDKALSAKA
                     RALARERWLYEQLLDALNAELGPLQRSAAALAELDVLATLAERAAELDYCRPRLVAEP
                     RLSITAGRHPVVERVSSDPFIANDLELDDRRRMLIITGPNMGGKSTYMRQVALIVLLA
                     HIGSFVPAAAAEIPLVDRIFTRIGSSDDLAGGRSTFMVEMAETATILHNATERSLVLM
                     DEIGRGTGTFDGLSLAWAAAARLASRQRAYTLFATHYFELTALPEVVEGVANVHLDAT
                     EYADRIVFLHSVRPGPASRSYGLQVAALAGVPQAVIAEARRKLQELESPHALAAVATT
                     SGAPAQGQLLFDADPLRDALLERLRALDPDALSPREAHALLYELHGAAREIY"
BASE COUNT          498 a         1166 c         1255 g          563 t
ORIGIN      
        1 tgcgcgagat cctcgagcgc caggggctca cgggcgacgc cgttttcagc tgagtttcga
       61 tgcgcgccgc atcaggtgcg gcggctacgc gtattgatta aatcgctccc ggcgataaga
      121 gcgtgcgctt tccgtctggg tagtgtactg cccgccagtg acatgacggc tctcccgctc
      181 ggacacgccg tgaatacgtc cctgtaggct cgactgtcgc catccaggcg acagacggtc
      241 ctcgcatgac agccgtcatg tcgctggctg cgtgcgtggt acgcatcgtt cttggcctgt
      301 gattacgtat gaacgcagga gcgggccatg cccgcgaccg ggtgtcctgc gctgcgcgtg
      361 agcgcggaga agcgctgcta cggcgtgatg cgtcaattgc gggggagatt cggcatgccc
      421 ctccccgcga caggaagggg cgaaggcggg acgctcagcg ctgcaggtgc tgcagcttgt
      481 cgggcacgcc gttccacttc tcggcgtcag cgagcttttc cttcatctcg gtgatgttcg
      541 gccacacctg cgccagttcg gcattcagtt ccaggaacac ctgctggtcg gcgggcaact
      601 catcctcgga gaagatcgcg tccaccgggc actcgggctc gcacagcgcg cagtcgatgc
      661 actcgtcggg atggatcacg aggaagttgg ggccttcgta gaaacagtcc accgggcaca
      721 cttccacgca gtcggtgtgc ttgcagttga tgcagttctc tccaacgacg aaagtcatgt
      781 cgcgcggcct cttgctgacg ggtgaaacgg gctccaaaag cgcgcgcagt ctagcatgcg
      841 gccccggtgc tgtcagacgc gcatttcgat gaattttcag taaatttcgc gtgccgcacc
      901 gtgcagttcg tagagcagcg cgtgcgcctc gcgcgggctg agcgcatcgg gatcgagcgc
      961 gcgcaggcgc tcgagcagcg catcacggag cgggtcggcg tcgaacagca gctggccctg
     1021 ggcgggtgcg ccggaggtcg tcgccacggc agcgagcgcg tggggcgact ccagctcctg
     1081 gagcttgcgc cgcgcctcgg cgatcacggc ctggggcacg ccggccagcg cggccacctg
     1141 caggccgtag ctgcgactgg cgggaccggg gcgtaccgag tgcaggaaca cgatgcggtc
     1201 ggcgtactcg gtggcgtcga ggtgcacgtt ggcgaccccc tccaccacct ccggcagcgc
     1261 ggtgagctcg aagtagtgcg tggcgaacag cgtgtaggcg cgctgccggc tggcgagccg
     1321 cgcggcggcc gcccacgcca gcgacagccc gtcgaaggtg ccggtgccgc gcccgatctc
     1381 gtccatcagc accaggctgc gctcggtggc gttgtgcagg atggtcgcgg tctcggccat
     1441 ctcgaccatg aaggtggagc gtccgcccgc gaggtcgtcc gaggagccga tgcgcgtgaa
     1501 gatgcggtcg accagcggga tctcggcggc cgcggcaggc acgaaactgc cgatgtgcgc
     1561 cagcagcacg atcagcgcga cctggcgcat gtaggtcgat ttgccgccca tgttcggccc
     1621 ggtgatgatc agcatgcgcc gccggtcgtc gagctcgagg tcgttggcga tgaagggatc
     1681 gctggagacg cgttccacca ccgggtggcg cccggccgtg atgctgagtc gcggttcggc
     1741 gaccagccgc ggccggcagt agtccagttc ggccgcgcgt tcggccagcg tcgcgagcac
     1801 gtccagctcg gcgagggcgg cggcgctgcg ctgtagcggg ccgagttccg cgttcagcgc
     1861 gtccagcagt tgctcgtaaa gccagcgctc gcgtgccagc gcgcgcgcct tcgcgctcag
     1921 cgccttgtcc tcgaaggcct tcagttccgg cgtgatgtag cgctcggcgt tcttcagcgt
     1981 ctggcggcgg atgtagtccg ccggcgcgtt gaccgactgg gcgcggctga tctcgatgta
     2041 gtagccgtgc acccggttgt agccgacatg caggttcggc agcccggtgc gttcgcgctc
     2101 gcgcgcctcc agtgccacca ggaagtcgtg agccccggtg ctgagcaggc gcagttcgtc
     2161 gagctcggcg tcgtagccgg gggcgatcac gccgccctcg cgcaccacca ccggcgggtt
     2221 ctcgaccagc gcgcacttca gcatggtcgc cagctgcggg aactcgctca ccgccgcgag
     2281 cagttgccgg ctcagtggtg cttccagcgc ggcgcaggcc gcgtgcagca cgggcaggct
     2341 ctcgagcgcg gcgccgaggc ggctgagatc gcgcgggcgc gccgagcgca acgccacgcg
     2401 cgccaggatg cgctcgatgt cgccgaccgc cgccagcgcc tcgcgtggcg ccaggtaccg
     2461 cgcgtcatcg acgagttcgg cgatcgcgcc ctggcgcgcc tcgagctcgg ccagccggcg
     2521 cagggggcgg cgcagccagc gccgcagcca gcggctgccc atggcggtct tcgtggtgtc
     2581 gagcacccag gccagcgtgt attcgtcgcc gccggtgaaa ttggtgtcga tctcgaggtt
     2641 gcgcagcgag gcggcgtcga gcgccaccgc gtcctcgcgc gaatcgggca ccagcgcgct
     2701 cacgtgcggc agctcgccgc gctgcgtctc gcgcgcgtag cgcagcaggc agccggcggc
     2761 cgcgatgccg agctccatct gcgcgcagcc gaaaccgacc aggtcgtgcg tgccgaactg
     2821 cgcgttcagc agttccgtgg cgctgcgtac ttcgaactcc cacggcgcgc gtgtgcgcaa
     2881 gccgttgatg ctcgccagcg cggggcggcg cgccgtgcct tcggcggcca gcagttccgc
     2941 cggttgcagg cggcgcagct ccgccgcgag cgcctcctcg ccgtcgacct cgagcacctg
     3001 gaagcgcccg ctggagagat ccagcgaact gatgccgaaa cgcgaacccg attccgcgac
     3061 cgcgaccagc aggttgtcgc gcgcggcgtc gagcagcgat tcgtcggtga gcgtccccgg
     3121 ggtcacgatg cgcgtgacct cgcgctcgac cggccccttg ctggtcgccg gatcgccgat
     3181 ctgctcgcag atggccaccg agacgccggc gcgcaccagc cgcgcgaggt agccctcgac
     3241 cgagtggtag ggcacgccgg ccatcgggat cggcacgccg gccgaggcgc cgcggctggt
     3301 cagcgtgatg tcgagtagcg cggcggcgcg tttcgcgtcg tcgtggaaca actcgtagaa
     3361 gtcgcccatg cggtagaaca gcagctcgtt gggatgccgg gacttgatgc gcaggtactg
     3421 ctgcatcatc ggtgtgtgca gggccgctgc tgctcccggg agcgggggta tgcgggagtc
     3481 gg
//
LOCUS       DAORUQ010000266         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_37324_length_3422_cov_6.487080, whole genome shotgun sequence.
ACCESSION   DAORUQ010000266 DAORUQ010000000
VERSION     DAORUQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37324_length_3422_cov_6.487080"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLW13_15205"
     CDS             <1..325
                     /locus_tag="PLW13_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71775.1"
                     /translation="VYIMDGEILADGTIAELLTAAGLHTFTVQGPGVTRLRPRLERQP
                     GVQQVAPFGDTLHVTGTDRAALLAAIEPLRADSAWTWAEGETSLEDLFIHLAAQRRRG
                     VADAR"
     gene            315..1490
                     /locus_tag="PLW13_15210"
     CDS             315..1490
                     /locus_tag="PLW13_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71776.1"
                     /translation="MRARPGLLAGLAAQLRHLSLRRIGAVLIKEFIQLRRDRMTFGMI
                     LGLPLAELVLFGFAINSDPKQLPTAVFVQDHSAYSRAVVNAFENSGYFRIVRENHSQA
                     EGDRQLLRGEVTFVLTIPEEFSRALLRNERPQVLLEADATDPAAASNALAAATQLAHT
                     ALAHQHLAAAGVEAGAPPFAVVAHRSYNPEGISQYNTVPGLLGVILTMTMVMMPALAL
                     TRELERGTMENLLALPVSPAEIMFGKVLPYIGMGAVQVLIVLGVARWVFAVPMLGSYP
                     LLFAMVAVFVVSLATMGYLISTVARNQMQALQMTFFFFMPSMLLSGFMFPFHGLPGWA
                     QAIGEVLPLTHMLRIVRGIMLKGAGFVEVAPHLWPLLLFLLVVTMVALTRFRRTLDA"
     gene            1628..2371
                     /locus_tag="PLW13_15215"
     CDS             1628..2371
                     /locus_tag="PLW13_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQY71777.1"
                     /translation="MSENPGFGFAPFKRAPAYQVVSEKIREAILDGRIPAGKLLPTET
                     ALAAQFGVTRSTLREAIRLLEQSGLLGRAGRKRLEVRLPSLESASRSVNAAMQMHRVT
                     FKDLWEVSMGLEPLAARLACATAGADFKHRLAANLERTAAALDDDEELLEAEIEFHDL
                     VAQSTGNHALLLARQPLNQLFYPAFRPVIERLKPGRRILESHRRIHEAILANDPDTAA
                     QWAEKHMLDFRRGILMAKLDFEGAVNPHT"
     gene            2409..3422
                     /locus_tag="PLW13_15220"
     CDS             2409..3422
                     /locus_tag="PLW13_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY71778.1"
                     /translation="MNNVLTPRLELVICVLFAAALLLTLQLHLLPSLLAGLLVYALVH
                     MLVPLLRLPVFGHEGARLVAVTLIATVVIALIALAAVSLSSVLRHSGESIPVLVHRMA
                     EIVDDSRASLPPWLFEYLPANADALRAAVVTWLKENAEFFQVAGTGFGRALAHILLGM
                     VIGALLSLEAASSRHGSAPLARCLAERATRLSDAFRRVVFAQFWISAINTALTAVFLV
                     AVLPLLGVELPFTKTLLLITFVAGLIPILGNLISNTAICIVALSQSLVLALGVLLYLV
                     LIHKLEYFLNARIVGSHIQARAWEMLLAMLVMEAAFGIPGLIAAPIFYAYAKDELASR
                     KLI"
BASE COUNT          484 a         1231 c         1132 g          575 t
ORIGIN      
        1 cgtctacatc atggacggcg agatcctcgc cgacggcacc atcgccgagc tgctcaccgc
       61 tgccgggctg cacaccttca cggtgcaggg ccccggcgtc acccggctgc gcccgcgcct
      121 cgagcgccag cccggcgtgc aacaggtcgc gcccttcggc gacaccctgc acgtgaccgg
      181 caccgaccgc gcggcgctgc tcgccgcgat cgagccgctg cgcgccgaca gcgcatggac
      241 ctgggccgag ggggaaacct cgctggagga cctgttcatc catctggcgg cgcaacgccg
      301 ccgcggggtt gccgatgcgc gctaggccgg gcctgctcgc gggcctcgcg gcacagttgc
      361 gccacctctc gctgcggcgc atcggcgcgg tgctgatcaa ggagttcatc cagctcaggc
      421 gtgaccgcat gaccttcggc atgatcctcg gcctgccgct cgccgaactg gtgctgttcg
      481 gcttcgcgat caacagcgac ccgaagcagc tgccgacggc cgtgttcgtg caggatcaca
      541 gcgcctacag ccgcgccgtc gtcaacgcct tcgaaaactc cggctacttc cgcatcgtgc
      601 gcgagaacca cagccaggcc gagggggacc ggcagctgct gcgcggtgaa gtcacctttg
      661 tgctcacgat tcccgaggaa ttctcgcgcg cactgttgcg caacgagcgc ccgcaggtgc
      721 tgctggaagc cgacgccacc gatccggcgg cggcgtcgaa cgcgctggcc gcggccacgc
      781 agctcgcgca cacggccctc gcgcaccagc accttgccgc ggccggggtc gaggcgggag
      841 cgccgccgtt cgcggtggtg gcgcaccgca gctacaaccc cgagggcatc tcgcagtaca
      901 acaccgtgcc ggggctgctc ggcgtgatcc tcaccatgac gatggtcatg atgccggcgc
      961 tggcgctgac gcgcgaactg gagcgcggca ccatggagaa cctgctggcg ctgccggtgt
     1021 cgccggcgga gatcatgttc ggcaaggtgc tgccctacat cggcatgggc gcggtgcagg
     1081 tgctgatcgt gctcggggtc gcgcgctggg tgttcgcggt gccgatgctc ggctcctacc
     1141 cgttgctgtt cgcgatggtg gccgtgttcg tcgtcagcct ggccacaatg ggctacctga
     1201 tctcgaccgt ggcgcgcaac cagatgcagg cgctgcagat gaccttcttc tttttcatgc
     1261 cctcgatgct gctgtcgggg ttcatgttcc cgtttcacgg cctgccgggc tgggcgcagg
     1321 ccatcggcga ggtgctgccg ctcacgcaca tgctgcgcat cgtgcgcggc atcatgctga
     1381 agggcgcggg attcgtcgag gtcgcgccgc acctctggcc gctgctgctg ttcctgctgg
     1441 tggtgacgat ggtcgcgctg acacgctttc gccgcaccct cgacgcctga caccggccgc
     1501 cgggcgcgcg gccggcctcg ctcacattgc tgcccgttcc cagcgttgac acattgtcgg
     1561 acaacagaca ctatcgcgac gcagccatcg cgcatccacc ggtcgcgcgg gctttcggaa
     1621 ccgttgcatg tcagagaacc ccggcttcgg cttcgcgccg ttcaaacgtg ccccggccta
     1681 ccaggtcgtg agcgagaaga tccgcgaggc gatcctcgac gggcgcatcc ccgccggcaa
     1741 gctgctgccc accgagaccg cgctggccgc gcagttcggc gtgacgcgct cgacgctgcg
     1801 cgaggccatc cggctgctcg aacagagcgg cctgctgggg cgcgccgggc gcaagcgcct
     1861 cgaggtgcgc ctgccctcgc tggaatcggc ctcgcgctcg gtcaacgccg cgatgcagat
     1921 gcaccgcgtc accttcaagg acctgtggga agtctcgatg ggactggagc cgctggcggc
     1981 gcgcctcgcc tgcgccacgg ccggcgcgga cttcaagcac cgccttgcgg ccaacctcga
     2041 gcgtacggcc gcggcgctgg acgacgacga ggagctgctg gaagccgaga tcgagttcca
     2101 cgacctggtg gcgcagtcaa cgggcaacca cgccctgctg ctggcgcgcc agccgctgaa
     2161 ccagctgttc tacccggcct tccgcccggt gatcgagcgc ctcaagccgg ggcggcgcat
     2221 cctcgagtcg caccggcgca tccacgaggc cattctcgcc aacgatcccg acacggccgc
     2281 gcagtgggcc gagaagcaca tgctcgattt ccgccgcggc atcctgatgg cgaagctcga
     2341 tttcgagggg gcggtgaacc cgcacacttg acgtgcgccc cgggcacaag ccaagctccg
     2401 ccccgcacat gaacaacgtc ctcacgcccc gcctcgaact cgtcatctgc gtgctcttcg
     2461 ccgccgcgct gctgctgacg ctgcaactgc acctgctgcc ctcgctgctc gcgggtctgc
     2521 tggtctacgc gctggtgcac atgctggtgc cgctgctgcg cctgccggta ttcggccacg
     2581 agggcgcgcg cctggtcgcg gtgacgctga tcgccaccgt ggtgatcgcg ctgatcgcgc
     2641 tggccgccgt gagcctgagc tccgtgctgc gccacagcgg cgaaagcatc ccggtgctgg
     2701 tgcaccgcat ggccgagatc gtcgacgatt cgcgcgccag cctgccgcca tggctgttcg
     2761 agtacctccc ggcaaatgcc gacgcgttgc gcgcggcggt cgtgacctgg ctgaaggaaa
     2821 atgcggagtt cttccaggtg gcgggcaccg gctttggccg cgcgctggcg cacatcctgc
     2881 tcggcatggt gatcggggcg ctgctgtcgc tggaggccgc gagctcgcgc cacggctcgg
     2941 ccccgctggc gcgctgcctg gccgaacgcg cgacgcggct gagcgacgcc ttccgccgcg
     3001 tggtgttcgc gcagttctgg atctccgcca tcaacacggc actcaccgcc gtgttcctgg
     3061 tggcggtgct gccgctgctc ggcgtcgagc tgccgttcac caagacgctg ttgctgatca
     3121 cattcgtggc cgggctgatc cccatcctcg gcaacctgat ctccaatacc gccatctgca
     3181 tcgtggcgct cagccagtcg ctggtcctgg cgctcggcgt gctgctctac ctggtgctga
     3241 tccacaagct cgagtacttc ctgaacgcgc gcatcgtggg cagccacatc caggcgcgag
     3301 catgggaaat gctgctggcg atgctggtga tggaagccgc cttcggcatc cccgggctga
     3361 tcgccgcccc gatcttctac gcctatgcca aggacgagct cgcgagccgc aagctcatct
     3421 ga
//
LOCUS       DAORUQ010000267         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_37735_length_3398_cov_6.745139, whole genome shotgun sequence.
ACCESSION   DAORUQ010000267 DAORUQ010000000
VERSION     DAORUQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37735_length_3398_cov_6.745139"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLW13_15225"
     CDS             complement(<1..1007)
                     /locus_tag="PLW13_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007986269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71779.1"
                     /translation="MKLSFSEQDERFRAEVAGWLRENLRGEFEQIRFRGGPGDEHMFS
                     EERKHWERRMAAGGWTCIGWPTEYGGRGASIEQQVIFHEEYARAGGPGRVGHIGEGLA
                     GPTIIAFGTEEQKARLLPGIRAGTELWCQGYSEPGAGSDLAAVKTRARLDEASGNWLI
                     NGQKVWTSLAHESQWCFVIARTDPDSVGHAGLGFFLVKMDQPGVTVRPIEQITGTSEF
                     NEVFFDDAVCEANDMLGKPGDGWKIGMALLGFERGTSTLGQQMLFQNELDEIVRIARE
                     NGRGKDPALRQRIADAWIGLRIMRYNAMRMLSGPQDGSLAREATAYKLYWAVWHRNLG
                     KL"
     gene            complement(1004..2161)
                     /locus_tag="PLW13_15230"
     CDS             complement(1004..2161)
                     /locus_tag="PLW13_15230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71780.1"
                     /translation="MEFAFTEEQQMIRETAAQFLADNCDPAAVRRAMATESGFEPQMW
                     QRIAGEMYWTAIHIPEEYGGLGLGYVELVAVMEQMGRYLLCAPYFSSVCLGANALLLA
                     GSEAQKVEYLPQIADGSMRATLAWCDASGRWDADGVSATATGKGDAFVLDGSYRYVVD
                     GHTADLLVVAARAPGSSGGAGLSLFVLPADTAGVTRTWLPTMDQTRKLAELRLDNVSV
                     PAEALLGAEGGAHAALGKTLALASVALAAEQAGGAQQILDMAVAYTKERQQFGRAIAS
                     FQAIKHKAADMMLKAEAAKSAVYYAACVAQEALTGGAQGAQLAEAASLAKAYCSDTYF
                     FNAGTAMQLHGGVGFTWEYDVHLYFKRARASESFLGDGAEHRERIAVELGL"
     gene            complement(2418..3299)
                     /locus_tag="PLW13_15235"
     CDS             complement(2418..3299)
                     /locus_tag="PLW13_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71781.1"
                     /translation="MAGICDGRVVIITGAGGGLGREYALAFAKEGASVVINDINPAAA
                     ERTVGEITAMGGKAVPNSNDITSHADAALIVKSAIDAFGDLHVVVNNAGICRDRMFVS
                     LSEQDWDEVIRVHLKGHFCISSHACNYWRAQAKDGKPVSGRIINTSSGAGLQGSIGQA
                     NYSAAKGGIATLTLVQAAELRRYNITANALAPSARTGMTEGVFAEMMKKPEDGSFDHY
                     DPANVAPLVVWLGSELSGHVTGRVFEVEGGRIAIGDGWRSTTGVDRKARWAPAEITGA
                     MEQLLAAEVPAQKVFGT"
BASE COUNT          499 a         1216 c         1098 g          585 t
ORIGIN      
        1 agcttgccga ggttgcggtg ccacacggcc cagtacagct tgtaggccgt ggcctcgcgc
       61 gcgagcgagc cgtcctgcgg ccccgagagc atgcgcatcg cgttgtagcg catgatgcgc
      121 agcccgatcc acgcgtccgc gatgcgctgg cgcagcgccg gatccttgcc gcggccgttc
      181 tcgcgcgcga tgcgcacgat ctcgtcgagc tcgttctgga acagcatctg ctggcccagc
      241 gtcgaggtgc cgcgctcgaa gccgagcagc gccatgccga tcttccagcc gtcgccgggc
      301 ttgccgagca tgtcgttcgc ctcgcacacg gcgtcgtcga agaacacctc gttgaactcc
      361 gaggtgccgg tgatctgctc gatcgggcgc acggtaacgc cgggctggtc catcttcacg
      421 aggaagaagc cgagccccgc gtgcccgacc gaatcgggat cggtgcgcgc gatcacgaag
      481 caccactgcg actcgtgcgc cagcgaggtc cacaccttct ggccgttgat caaccagttg
      541 ccgctcgcct cgtccaggcg cgcccgggtc ttcacggcgg ccagatcgga accggcgccc
      601 ggctcggaat agccctggca ccacagctcg gtgccggcgc ggatgccggg cagcaggcgc
      661 gccttctgct cctcggtgcc gaaggcgatg atcgtggggc ccgcgagccc ctcgccgatg
      721 tggcccacgc ggcccgggcc gccggcgcgc gcgtattcct cgtggaatat cacctgctgt
      781 tcgatcgagg cgccgcgccc accgtattcg gtcggccagc cgatgcaggt ccagccgccc
      841 gcggccatgc gtcgctccca gtgcttgcgc tcctcggaga acatgtgctc gtcgccgggg
      901 ccgccgcgaa aacggatctg ctcgaactcg ccgcgcaggt tctcgcgcag ccagccagcc
      961 acctcggcgc gaaagcgctc gtcctgttcg ctgaaactca gtttcatagt cccaactcca
     1021 cggcaatgcg ctcgcggtgc tccgcaccgt cgccaaggaa actctcgctg gcgcgcgcgc
     1081 gcttgaagta caggtgcacg tcgtattccc aggtgaagcc gacgccaccg tgcaactgca
     1141 tcgcggtgcc ggcattgaag aaataggtgt cgctgcagta ggccttggca aggctggccg
     1201 cctcggccag ttgcgcgccc tgcgcaccgc ccgtcaatgc ctcctgcgcc acgcaggcgg
     1261 cgtagtagac ggccgacttc gcagcctcgg ctttcagcat catgtccgcg gccttgtgct
     1321 tgatggcctg gaagctcgcg atcgcgcggc cgaactgctg gcgctccttc gtgtaggcga
     1381 ccgccatgtc gaggatctgc tgcgcaccgc cggcctgctc ggccgcgagc gcgacgctcg
     1441 ccagcgcgag ggtcttcccg agtgctgcgt gcgcaccgcc ttcggcgccg agcagcgcct
     1501 cggccgggac gctcacgttg tccaggcgca gctcggcgag cttgcgcgtc tggtccatcg
     1561 tcggtagcca ggtgcgcgtg acgccggcgg tgtccgccgg cagcacgaac aggctgaggc
     1621 cggcaccgcc gctgctgccc ggcgcgcgtg ccgcgacgac cagcaggtcc gccgtgtgac
     1681 catcgaccac gtagcggtag ctgccatcga gcacgaaggc atcgcctttg cccgtggccg
     1741 tcgccgaaac gccgtccgcg tcccagcggc ccgaggcatc gcaccacgcc agcgtggcgc
     1801 gcatcgagcc gtcggcgatc tgcggcaggt actcgacctt ctgcgcctcg ctgccggcga
     1861 gcagcagtgc gttggcgccg aggcataccg aggagaagta cggcgcgcac agcaggtagc
     1921 gacccatctg ttccatcacg gcgaccagtt cgacatagcc gagcccgagt ccgccgtact
     1981 cctcgggaat gtggatcgcg gtccagtaca tctcgcccgc gatgcgctgc cacatctgcg
     2041 gctcgaagcc gctctccgtg gccatcgcgc ggcgcaccgc ggccgggtcg caattgtctg
     2101 cgaggaactg cgccgcggtt tcgcggatca tctgctgttc ttcggtgaag gcaaattcca
     2161 tggggattcc tgctaatcga tcacctgttg attgtccggc aacccaatgc tgcgcctggc
     2221 ggcgccacaa ctgtccggct gaagccggac ctacgtcttc ctgccagcgg cacgccagcc
     2281 ggcaccgggg cgagtgcgct cctgcagggg ccgtgcgccg cagggatgcg gcgctcgagc
     2341 gtacagggac gtattcacag cgtgtcccgg caggaacgca gtcgtcgcgg tgacggcaat
     2401 gctcagccaa aacacactca ggtgccgaaa accttctgcg ccggcacctc ggccgcgagc
     2461 agctgttcca tcgcgccggt gatctcggcg ggcgcccagc gcgccttcct gtcgacgccc
     2521 gtcgtgctgc gccagccgtc gccgatcgcg atgcgaccgc cctcgacctc gaatacgcgg
     2581 cccgtcacgt ggcccgaaag ctcgctgccg agccacacca ccagaggcgc gacgttggcc
     2641 ggatcgtagt ggtcgaagct gccgtcctcg ggcttcttca tcatctcggc gaacacgccc
     2701 tcggtcatgc cggtgcgcgc cgacggcgca agcgcgttgg ccgtgatgtt gtagcggcgc
     2761 agttcggccg cctgcaccag cgtcagcgtg gcgataccgc ccttggccgc cgagtagttt
     2821 gcctggccga tcgagccctg caggccagcg cccgaactgg tgttgatgat gcgtccgctg
     2881 accggcttgc cgtccttcgc ctgggcgcgc cagtagttgc aggcgtggct gctgatgcag
     2941 aagtggccct tcaggtgcac acggatcact tcgtcccagt cctgttcgct caggctcacg
     3001 aacatgcggt cacggcagat gccggcgttg ttcaccacga cgtgcaggtc gccgaaagca
     3061 tcgatcgcgc tcttgacgat cagcgcggcg tcggcgtggc tcgtgatgtc gttcgaattc
     3121 gggacagcct tgcctcccat cgccgtgatc tcgcccacgg tgcgctcggc cgccgcgggg
     3181 ttgatgtcgt tgatcacgac gctcgcgcct tccttcgcga aagcgagcgc gtactcacgc
     3241 ccgagcccgc cgccggcgcc ggtgatgatc actacacgtc cgtcacagat tcctgccatt
     3301 tcccttactc ccctgtcagt tcagttcgtt cgatcacagc ccacgattcg agctgtcgat
     3361 gtcctgtgca gtcctgcccg gccagcgcat ggcgggcc
//
LOCUS       DAORUQ010000268         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_37896_length_3389_cov_5.024295, whole genome shotgun sequence.
ACCESSION   DAORUQ010000268 DAORUQ010000000
VERSION     DAORUQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37896_length_3389_cov_5.024295"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="aroC"
                     /locus_tag="PLW13_15240"
     CDS             complement(<1..690)
                     /gene="aroC"
                     /locus_tag="PLW13_15240"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQY71782.1"
                     /translation="MSGNSIGRFFTLTSFGESHGPAIGGIVDGCPPGLALCAEDLQPD
                     LDRRRPGSSRYTTQRQEEDRVRILSGVFEGVTTGAPIGLLIENTDQRSKDYTAIKDTF
                     RPNHADYAYTQKYGLRDYRGGGRSSARETAVRVAAGAIAKKYLRERLGVTVRGYLAQL
                     GPLRAAALDWDTVETNPFFCPDAALVPQLEAYMQALIKAGDSIGARINVVASGLPPGL
                     GSHIQGDRRLDA"
     gene            complement(769..1695)
                     /gene="prmB"
                     /locus_tag="PLW13_15245"
     CDS             complement(769..1695)
                     /gene="prmB"
                     /locus_tag="PLW13_15245"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQY71783.1"
                     /translation="MGKVETGHEAPQELQTVRDFLRWAVSAFNAAGVAYGHGTDNAWD
                     EARWLVLGALHLPFDSPDWVLEARLGAAERAHLGALLARRVGERVPVAYLLGEAWFAG
                     LRFRVDPSVLIPRSPLAELIATRCEPWIDAQVEPRRILDLCCGGGCIGIAAALAWPGA
                     EVVLGDISPAALALARENVALHGVAERVRVVESDLFAALGDELFDLILTNPPYVDAAD
                     MDALPAEFRHEPRLGLAAGADGLDIARRILAEAADHLEPEGLLVLEVGNSADALERAF
                     PQLPFLWPDFEHGGHGIALIHAREIREHRRAP"
     gene            complement(1682..2551)
                     /locus_tag="PLW13_15250"
     CDS             complement(1682..2551)
                     /locus_tag="PLW13_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71784.1"
                     /translation="MSTRAAPREHAITVRGARLAWRQWGEDHEYPVLALHGWLDNAAS
                     FDALGPLLSGYRVIALDLPGHGLSDQRGAHGSYNIWDDLPDLVWLSAELGLARYALLG
                     HSRGAAIATLLAAIEPGAVSSLVLLDGMLAPMFSEEDTVAQLRQFAHDYGRRRSRGPR
                     VFAGVEEAVAARCAATGIDPRAAAMLVPRSLEPVPGGWRWRTDGRLRHASALKFTRGN
                     VRNVLEALQLPALQFRAAQGLGARQPDEECSGWLRGLALEEIDGSHHCHMLEQAPAIA
                     ARIQDFWRTCGQG"
     gene            complement(2548..>3389)
                     /locus_tag="PLW13_15255"
     CDS             complement(2548..>3389)
                     /locus_tag="PLW13_15255"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQY71785.1"
                     /translation="KVIAACVGNNCRVADVVGNFKVKKAPLPVYAVPTTAGTGSEVTA
                     VAVVTDTAARAKTPVVDGKLVPVMAALDASLMTGLPRAVTAATGMDALTHAVESYISG
                     FANADTRPLSVAAVRLIFANLGKVCANGADLEARQAMALASTYAGFAFTRASVGYVHA
                     IAHNLGGLYHTPHGLANAIVLPHVLDFSRDAAARQLAELAVEIGAATPAEPLAVRAQR
                     FIDRVRALARETGIPETLDALRTADIPVIARRAMDEAQGFYPVPRFMVQSECEALLAK
                     LLP"
BASE COUNT          504 a         1227 c         1203 g          455 t
ORIGIN      
        1 ggcatcgagc cgtcgatcgc cctggatgtg cgagccgagg cccgggggca gcccgctggc
       61 gaccacgttg atgcgcgcgc cgatcgaatc gccggccttg atcagcgcct gcatgtacgc
      121 ctcgagctgc ggcaccagcg ccgcatcggg gcagaagaag ggattggtct cgaccgtgtc
      181 ccagtcgagc gcagcggcgc gcagcgggcc gagttgcgcg aggtagccgc gcaccgtgac
      241 gccgaggcgc tcgcgcaggt acttcttcgc gatcgccccg gccgccaccc gcaccgcggt
      301 ctcgcgcgcc gaggaacgcc cgccgccgcg gtagtcgcgc aagccgtatt tctgcgtgta
      361 ggcatagtcg gcgtggttcg ggcggaaggt gtccttgatc gcggtgtagt ccttggagcg
      421 ctggtcggtg ttctcgatca gcagcccgat cggcgcaccg gtggtcacac cctcgaacac
      481 ccccgacagg atgcgcacgc ggtcctcttc ctggcgctgc gtggtatagc gcgaactgcc
      541 cgggcggcgc cggtcgagat cgggctgcag gtcttccgcg cagagcgcga gtccgggcgg
      601 gcagccatcg acgatgccgc cgatggccgg gccatggctc tcgccgaagc tcgtcagcgt
      661 gaaaaaacga ccgatactgt tgcctgacat gggaatgcgc gcgctccggt gaaacagggc
      721 gcgattatac ggagaatcgc ccgcacgcgg cccgactgcg gtgatttgtc acggcgcgcg
      781 gcggtgctcg cggatctccc gggcatggat cagcgcaatg ccgtggccgc catgctcgaa
      841 atccggccac aggaacggca actgcgggaa ggctcgctcc agcgcatcgg cgctgttgcc
      901 cacctcgagc accagcagcc cctcgggctc gaggtgatcg gccgcctcgg ccaggatgcg
      961 ccgcgcgata tcgaggccgt ccgcgccggc ggcgagcccg agccgcggct cgtggcggaa
     1021 ctccgcgggc agcgcgtcca tgtcggcggc gtccacgtag ggcgggttgg tgaggatcag
     1081 gtcgaagagc tcgtcaccga gcgcggcgaa aaggtcggac tcgaccacgc gcacgcgttc
     1141 ggccacgccg tgcagcgcca cgttctcgcg cgccagcgcc agcgccgccg gcgagatgtc
     1201 gccgagcacc acctccgccc cgggccaggc cagcgccgcc gcgatgccga tgcaaccgcc
     1261 gccgcagcac agatcgagga tgcgtcgtgg ctcgacctgc gcgtcgatcc acggctcgca
     1321 gcgcgtggcg atcaactcgg cgagcggcga gcgcgggatc agcaccgagg gatcgacgcg
     1381 aaagcgcagc ccggcaaacc acgcctcgcc gagcaggtag gccacgggca cgcgttcgcc
     1441 gacgcgccgc gccagcaatg cgccgaggtg cgcgcgttcg gcggcgccga gacgcgcctc
     1501 cagcacccag tcggggctgt cgaagggcag gtgcagcgcg cccagcacca gccagcgcgc
     1561 ctcgtcccag gcgttgtcgg tgccgtgccc gtaggccacc ccggcggcat tgaaggcact
     1621 caccgcccag cgcaggaaat cgcgcaccgt ctgcagttcc tgcggtgcct cgtgtccggt
     1681 ttcaaccttg cccacacgtc ctccagaagt cctgtatgcg cgcggcgatg gccggcgcct
     1741 gctcgagcat atgacagtga tgcgagccgt cgatctcctc gagcgcgagc ccgcgcagcc
     1801 agccgctgca ctcctcgtcg ggctgccgcg cccccaggcc ctgcgcggcg cgaaattgca
     1861 gcgccggcag ttgcagcgcc tcgagcacgt tgcgcacgtt gccgcgggtg aatttcagtg
     1921 ccgaggcgtg gcgcagccgt ccgtcggtgc gccagcgcca gccgccgggc acgggctcca
     1981 ggctgcgcgg caccagcatc gcggcggcgc gcgggtcgat gccggtcgcc gcgcaacgcg
     2041 ccgcgacggc ctcctccacg ccggcgaaga cgcgcgggcc gcgcgagcgg cgccgcccgt
     2101 agtcgtgcgc gaactggcgc agctgcgcca cggtatcctc ctcgctgaac atcggcgcga
     2161 gcatgccgtc gagcagcacc aggctgctca cggcgcccgg ctcgatcgcc gcgagcagtg
     2221 tggcgatggc cgccccgcgc gagtgcccga gcagcgcgta gcgcgccagc cccagctcgg
     2281 cgctcagcca caccaggtcc ggcaggtcat cccagatgtt gtagctgccg tgcgctccgc
     2341 gctgatcgct gagcccgtgc ccggggaggt ccagcgcgat cacgcgatag ccggaaagca
     2401 acggaccgag cgcatcgaag gaagcggcgt tgtccagcca gccgtgcagc gccagcaccg
     2461 ggtattcgtg gtcctcgccc cactggcgcc acgcaagccg ggcgcctcgc accgtgatcg
     2521 cgtgctcgcg cggcgcggcg cgcgtgctca cggcaacagc ttcgccagca gcgcctcgca
     2581 ctcggactgc accatgaagc gcggcaccgg gtagaagccc tgcgcctcgt ccatggcgcg
     2641 gcgcgcgatc acgggaatgt ccgccgtgcg cagcgcatcg agcgtctcgg gaatgccggt
     2701 ctcgcgtgcc agcgcgcgca cccggtcgat aaagcgctgc gcacgcacgg cgagcggctc
     2761 ggcgggcgtc gccgcgccga tctccaccgc cagctccgcg agttgcctcg cggccgcatc
     2821 gcgcgaaaaa tcgagcacgt gcggcagcac gatcgcgttg gccagcccgt gcggcgtgtg
     2881 gtacaggccg ccgaggttgt gcgcgatcgc gtgcacgtag ccgacgctgg cgcgcgtgaa
     2941 cgcgaagccc gcgtaggtcg aggcgagcgc catggcctgg cgcgcctcga gatcggcgcc
     3001 gttggcgcac accttgccga ggttggcgaa gatcaggcgc accgcggcca ccgagagcgg
     3061 acgcgtatcg gcgttggcaa agcccgagat gtaggattcg accgcgtgcg tcagcgcgtc
     3121 catgccggtg gccgcggtga ccgcgcgcgg cagcccggtc atcagcgagg catccagcgc
     3181 cgccatcacc ggcaccagct tgccatcgac caccggcgtc ttggcgcgcg ccgcggtgtc
     3241 ggtgaccacg gccacggccg tgacttccga gccggtgccc gccgtggtgg gcacggcgta
     3301 tacgggcagc ggcgccttct tcaccttgaa attgccgacc acgtcggcca cgcggcagtt
     3361 gttgccgaca caggcggcga tgaccttgg
//
LOCUS       DAORUQ010000269         3346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_38700_length_3346_cov_6.082042, whole genome shotgun sequence.
ACCESSION   DAORUQ010000269 DAORUQ010000000
VERSION     DAORUQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38700_length_3346_cov_6.082042"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1650
                     /gene="purF"
                     /locus_tag="PLW13_15260"
     CDS             124..1650
                     /gene="purF"
                     /locus_tag="PLW13_15260"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQY71786.1"
                     /translation="MCGIAGIVGRGHVNQQLYDALTVLQHRGQDAAGIVTCDQGVFKQ
                     RKANGLVRDVFRSEHMERLSGSYGIGHVRYPTAGSASAALAQPFYVNSPYGISLAHNS
                     NLTNSVELRAQVFQADLRHINTDSDSEVLLNVFAHELQILGKLVPDANDIFTAVAGVH
                     RRARGGYAVIALIAGHGIVAFRDPNGIRPLIFGERATGAGTEYMIASESVALDVLGFR
                     VIRDVAPGECVYIDVAGNLHTRQCADNPRLTPCIFEHVYFARPDSIIDGISVYKARLR
                     QGERLADKIKRLRPDHDIDVVIPIPDTSRVAAQVIAYELGVKFREGLMKNRYIGRTFI
                     MPGQAMRKKSVRQKLNPIELEFRDKNVLLVDDSIVRGTTCNEIIQMARDVGARRVYFA
                     SAAPPVRWPNVYGIDMPSSDELVAHGRTEEEVCELIGADWLVYQDLEDLVSCSRDGNP
                     EIRGFDCSVFNGEYVTGDVDAAYLERLHAARNDDSKARHEAAARAGDSALVGLHNHAT
                     "
     gene            1672..2880
                     /locus_tag="PLW13_15265"
     CDS             1672..2880
                     /locus_tag="PLW13_15265"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HQY71787.1"
                     /translation="MHDEERRAALARARGDTLAVRAGQVRSSEGEHSEALFLTSSYVF
                     DSAADAAARFAGTAPGNVYSRYTNPTVRTFEERLAALEGAEQAVATSSGMAAILTACM
                     AYLQAGQHIVCSRGVFGSTTVMLNRFIAKFGVAISYVSPTDYAGWEAAMRPETRMIFL
                     ETPSNPLNEVVDIERVAQIAHRRGALVVVDNCFLSPALQQPLQFGADVVIHSATKYID
                     GQGRCLGGVLCGSRELMEQAVGFLRSAGPCLSPFNAWVFLKGLETLRIRMEAHSRSAL
                     AIARWLQAQPGVTGVNYAGLASHPDHALACRQQRGFGGVLSFTVAGGQPAAWRFIDAT
                     RLLSITANLGDAKTTIVHPATTTHGRLSEEERRAQGITPELIRVAVGLEDVEDLEEDL
                     ARGLAAIRCA"
     gene            2976..>3346
                     /locus_tag="PLW13_15270"
     CDS             2976..>3346
                     /locus_tag="PLW13_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY71788.1"
                     /translation="MPATESPLAERGIHVQDIIEQAVRQIGRVLLGKDRQIRLALCAL
                     FSRGHLLIEDMPGMGKTTLSHALARVLGLEFARVQFTSDMLPGDILGVSVYDQRAQAF
                     NFHPGPVFTEVLLADEINRSTP"
BASE COUNT          531 a         1184 c         1126 g          505 t
ORIGIN      
        1 tggtggcagt cctcggtgct gctgccgcac ctgctgctgc tggagagcgg gttccggcaa
       61 ttcgccggca cgctgagcgc gctgctggcc ggcgcggtca actgagaacg gagggcgcga
      121 attatgtgcg gtatcgcggg catcgtcggt cgcggccacg tcaaccagca actctacgac
      181 gcgctgacgg tgctgcagca tcgcgggcag gatgccgcgg gcatcgtgac ctgcgaccag
      241 ggcgtgttca agcagcgcaa ggccaacggc ctggtgcgcg acgtgttccg cagcgagcac
      301 atggagcgcc tgagcggcag ctatggcatc gggcatgtgc gctatcccac cgcgggcagc
      361 gcgagtgcgg cgctggccca gccgttctac gtgaacagcc cctatggcat ctcgctcgcg
      421 cacaacagca acctgaccaa ctcggtcgag ctgcgcgcgc aggtgttcca ggcggacctg
      481 cgccacatca acaccgactc cgacagcgag gtgctgctga acgtgttcgc gcacgagctg
      541 cagattctcg gcaagctggt gcctgatgcg aacgacatct tcaccgcggt cgccggcgtg
      601 caccggcgcg cgcgcggcgg ctacgcggtg atcgcgctga tcgccggcca cggcatcgtg
      661 gccttccgcg accccaacgg catccgtccg ctgatcttcg gcgagcgcgc gaccggggcc
      721 ggcaccgagt acatgatcgc cagcgagagc gtcgcgctcg acgtgctggg ttttcgcgtg
      781 atccgcgacg tggcgcccgg cgagtgcgtg tacatcgacg tggcgggcaa cctgcacacg
      841 cgccagtgcg ccgacaatcc gcgcctcacc ccatgcatct tcgagcacgt gtacttcgcg
      901 cgcccggact cgatcatcga cggcatctcg gtgtacaagg cgcggctgcg ccagggcgag
      961 cgccttgccg acaagatcaa gcgcctgcgt cccgaccatg acatcgacgt cgtgatcccg
     1021 atccccgaca ccagccgcgt cgccgcacag gtcatcgcct acgagctcgg ggtgaagttc
     1081 cgcgaaggcc tgatgaagaa ccgctacatc ggccgcacct tcatcatgcc ggggcaggcg
     1141 atgcgcaaga agtccgtgcg ccagaagctg aacccgatcg agctcgagtt ccgcgacaag
     1201 aacgtgctgc tggtcgatga ttcgatcgtg cggggaacca cctgcaacga gatcatccag
     1261 atggcccgcg acgtgggcgc gcggcgcgtg tatttcgcct ccgccgcgcc gccggtgcgc
     1321 tggccgaacg tctacggcat cgacatgccc tcgtccgacg aactggtcgc ccacggtcgc
     1381 accgaggaag aggtctgcga gctgatcggc gccgactggc tggtctacca ggacctcgag
     1441 gacctggtgt cgtgctcgcg cgacggcaac ccggagatcc gcggcttcga ctgttcggtg
     1501 ttcaacggcg agtacgtgac cggcgacgtc gatgcggcgt atctcgagcg cctgcacgcg
     1561 gcgcgcaacg acgactccaa ggccaggcac gaagccgcgg cccgcgcggg cgattccgcg
     1621 ctggtcggcc tgcacaacca cgccacctga tccgcccggg ggagactgcc gatgcacgac
     1681 gaggaacggc gcgccgcact ggcgcgcgcg cgcggcgaca cgctggcggt gcgcgccggg
     1741 caggtgcgca gcagcgaggg tgaacactcc gaggcgctgt tcctcacctc gagctacgtc
     1801 ttcgattccg ccgccgatgc cgcggcgcgc tttgccggca ccgcgccggg caacgtctat
     1861 tcgcgctaca ccaacccgac cgtgcgcacc ttcgaggagc gcctcgccgc gctcgagggc
     1921 gccgagcagg cggtggccac gtcctcgggc atggccgcga tcctcaccgc ctgcatggcc
     1981 tatctgcagg cgggccagca catcgtgtgc tcgcgcggcg tgttcggctc gaccaccgtg
     2041 atgctgaacc gcttcatcgc gaagttcggc gtcgcgatca gctacgtctc gcccaccgac
     2101 tacgccggct gggaagccgc gatgcgcccc gagacgcgca tgatcttcct cgagacgccc
     2161 tcgaacccgc tgaacgaggt ggtggacatc gagcgcgtgg cgcagatcgc gcacaggcgc
     2221 ggcgcgctgg tggtggtgga caactgcttt ctcagccccg cgctgcagca gccgctgcag
     2281 ttcggtgccg acgtggtgat ccactccgcg accaagtaca tcgacggcca gggccgctgc
     2341 ctcggcggcg tgctgtgcgg cagccgtgaa ctgatggagc aggcggttgg cttcctgcgc
     2401 tcggccggcc cctgtctcag tccgttcaac gcatgggtgt tcctgaaagg gctggagacg
     2461 ctgcgcatcc gcatggaagc ccacagcagg agcgcgctgg cgatcgcccg atggctgcag
     2521 gcccagccgg gcgtgaccgg cgtgaactac gcgggactcg cctcgcatcc cgatcacgcg
     2581 ctcgcctgcc gccagcagcg cggcttcggc ggcgtgctgt cgttcacggt cgccggcggg
     2641 cagcccgcgg cgtggcgctt catcgacgcg acgcggctgc tctcgatcac cgcgaacctc
     2701 ggcgacgcca agaccaccat cgtgcacccg gccacgacca cgcacggccg tctctccgag
     2761 gaggagcgcc gcgcgcaggg catcacgccc gagctgatcc gcgtggcggt ggggctggaa
     2821 gacgtcgagg acctcgagga agacctcgcg cgcgggctcg ccgccatccg ctgcgcctga
     2881 ttcccgccta tccttaagcg cgaaccgcaa cacggtcgcg ggcatggccc gctcctacaa
     2941 tcgcgtcatc gccgttgcag gtgcaggagc gggccatgcc cgcgaccgaa agcccgctcg
     3001 ctgaacgagg aatccacgtg caggacatca tcgaacaggc ggtgcgccag atcgggcgcg
     3061 tgctgctcgg caaggaccgc cagatccgcc tcgcgctgtg cgcgctgttc tcgcgcgggc
     3121 acctgctgat cgaggacatg cccggcatgg gcaagaccac gctctcgcac gcgctggcgc
     3181 gcgtgctggg cctggagttc gcgcgcgtgc agttcaccag cgacatgctc ccgggcgaca
     3241 tcctcggcgt ctcggtctac gaccagcgcg cgcaggcctt caacttccat cccggcccgg
     3301 tgttcaccga ggtgctgctg gccgacgaga tcaaccgcag cacccc
//
LOCUS       DAORUQ010000270         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_38990_length_3332_cov_6.628624, whole genome shotgun sequence.
ACCESSION   DAORUQ010000270 DAORUQ010000000
VERSION     DAORUQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38990_length_3332_cov_6.628624"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="PLW13_15275"
     CDS             <1..1826
                     /locus_tag="PLW13_15275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY71789.1"
                     /translation="CSDENSTLEDIARIVATDPVLTARLLRQANSAAVGGQRPVAAIP
                     EAVKRLGLGAVRQLAMGFSLVDQYHTGPCTSFDYQRFWSHSLLMAVALQALGSLVRVA
                     SPDELFACGLMARIGCLSLATIHPVEYAELLEGCRDDAELAARERERLEADHNDVTVA
                     IFDSCGIPRSLAEPVFFHEAPERSNFTDGSRPHQLAHLFYLAKRVADMGLAPEGERSG
                     IIAELMLLGGKLGLDADGLGSVVDRVIGEWQQWAQLLKVPAHALPPFAAMAEAPAPRA
                     EAQQEDQPPLRVLVAGSDTTGRAEVMAMLAALPRCTVHAAENGREALALALEVMPQIV
                     VSDRRMPDVDGIGFCQALRATDWGQSMYVIMLTDTDAEDEVVQAFEAGADDCIAKPVN
                     ERALRARLRAALHYVRLLEAWERDRSQLKQFASELAISNRKLEQYALTDLLTGLPNRR
                     SGMDVLGQAWAASQRSGQALAVMMMDIDHFKSVNDTHGHAVGDKVLVEVGRLMRDALR
                     KGDHVCRMGGEEFLVINQGGELAATLQFAERLRQKIRAARIRIGDVELGTSISIGVAC
                     REKGMADPGNLILAADQALYAAKRGGRDRTCCNAGGRLQCR"
     gene            1917..3089
                     /locus_tag="PLW13_15280"
     CDS             1917..3089
                     /locus_tag="PLW13_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY71790.1"
                     /translation="MPYTNTADEHDALLGALYQGALEPTPWQSFLPCLLDALDAMVVS
                     LTLRPPRAGDSGLILNYLRPYTTADGHSFSKADPRDWPSTAYREQFFALDPFIDLPPL
                     TVVSLQELIPLAELRESEYYRQFLEPAGILYILGADAREPLGMEARLRVSRGPDDAPF
                     SKDDQRLLERLLPHLARALEINARLARTESERALYEGAVDQLAVGTIILDGHGRVLNS
                     NRVAGELLALADGMRVTDSELRFTDPAVGRQLQQVIEKVLDAHLRGAPAVVEALRVRR
                     PSGKGDFGLVIRPVPRSAWSEGQSSPTVAVFVSAPEVQSQTSELVIKQLFDFTPAESR
                     LALRLAGGQSLAEACEELCISQHTARAQLKTIFAKSGVTRQAELVRLILRSVATLA"
     gene            complement(3093..>3332)
                     /locus_tag="PLW13_15285"
     CDS             complement(3093..>3332)
                     /locus_tag="PLW13_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48 Ste24p"
                     /protein_id="HQY71791.1"
                     /translation="FYDTMFLSAIRSFRPMSGADRDAVLGINLHYIRAEPGMSFAKLA
                     ETSPLKDHAEEHLRLVNGYYPRGEPQPGEWIKIFR"
BASE COUNT          510 a         1154 c         1169 g          499 t
ORIGIN      
        1 cctgcagcga tgagaacagc accctggagg acatcgcgcg catcgtcgcc accgatcccg
       61 tgctgaccgc tcgcctgttg cggcaggcca actcggccgc cgtcggcggg cagcgccccg
      121 tggccgcgat ccccgaggca gtgaagcggc tggggctggg tgccgtgcgc cagctcgcga
      181 tgggcttctc gctggtcgac cagtaccaca cggggccctg caccagcttc gactaccagc
      241 gcttctggtc gcattcactg ctgatggcag tggccctgca ggcgctgggc tccctggtgc
      301 gtgtcgcctc gcccgacgag ctctttgcct gcggactgat ggcgcgcatc ggctgcctct
      361 ccctcgcgac catccatccc gtggagtacg cggaactgct cgagggctgc cgcgacgacg
      421 cggagctggc cgcgcgggaa cgcgagcgcc tggaggccga tcacaacgat gtcacggtcg
      481 cgatattcga cagctgcggc atccccaggt cgctggcgga accggtgttc ttccacgagg
      541 cgccggagcg ctcgaacttc accgacggat cgcgtccgca ccagctcgcg cacctcttct
      601 atctcgccaa gcgcgtggcc gacatgggac tcgcgcccga aggcgagcgc agcggcatca
      661 tcgccgagct gatgctgctc ggcggcaagc tgggcctgga cgccgacggc ctgggcagcg
      721 tggtcgaccg ggtcatcggc gagtggcagc agtgggcaca attgctcaag gtgcccgcgc
      781 acgcgctgcc gccatttgcc gccatggccg aagcgccggc gcccagggcc gaggctcagc
      841 aggaagacca gcccccgctg cgcgtgctgg tggcgggcag cgacaccacc gggcgcgccg
      901 aagtgatggc catgctcgcc gccctgccgc gctgcacggt tcacgcggcc gaaaacggcc
      961 gcgaggcgct ggccctggcg ctggaagtga tgccccagat cgtggtcagc gaccggcgca
     1021 tgccggatgt ggacggtatc ggcttctgcc aggccctgcg cgccaccgac tggggccagt
     1081 cgatgtacgt gatcatgctg acggacaccg acgcggaaga cgaggtcgtg caggcgttcg
     1141 aggcgggtgc cgatgactgc atcgccaagc ccgtcaacga acgcgcgctg cgcgcgcgcc
     1201 tgcgtgccgc cctgcattac gtgcgcctgc tggaagcctg ggagcgcgac cgctcgcagc
     1261 tcaagcagtt tgcctcggag ctggccatca gcaaccgcaa gctggagcag tatgcgctga
     1321 ccgatctgct gaccggcctg cccaacaggc gctcgggcat ggacgtgctg ggccaggcct
     1381 gggccgcatc gcagcgctcc ggacaggcgc tggcggtgat gatgatggat atcgatcatt
     1441 tcaagagcgt gaacgacacc catggccacg cggtgggcga caaggtgctg gtggaagtcg
     1501 ggcggctgat gcgggacgcg ctgcgcaagg gcgatcacgt ctgccggatg ggtggcgagg
     1561 agttcctggt gatcaaccag ggcggcgagc tcgccgcgac gctgcaattc gccgagcgcc
     1621 tgaggcagaa gatccgcgcc gcgcgcatcc gcatcggtga cgtggagctg ggcaccagca
     1681 tcagcatcgg ggtggcatgc agggagaagg gcatggccga cccgggcaac ctgatcttgg
     1741 ctgccgacca ggcgctctac gccgccaagc gcggcggacg cgatcgcacc tgctgcaacg
     1801 cgggcgggcg gctgcaatgc cgctgagcga ggggtcgcgc gcaccggatc ccctcgcgcc
     1861 ggtgcccggc tgcgagccgg actgccggcg ccgccggcga cggttacact agcgccatgc
     1921 cctacaccaa caccgccgac gaacacgatg cgctgctcgg cgcgctctac cagggcgcgc
     1981 tggagcccac accatggcag agcttcctgc cgtgcctgct cgatgcgctg gacgcgatgg
     2041 tggtgtcgct gacgctgcgc ccgccgcgcg ccggcgacag cgggctgatc ctgaactacc
     2101 tgcgccccta caccaccgcg gacggccaca gtttcagcaa ggccgacccg cgcgactggc
     2161 cttccaccgc ctatcgcgag cagttcttcg cgctcgatcc gttcatcgat ctgccgccgc
     2221 tgacggtggt gagcctgcag gaactgattc cgctggcgga gctgcgcgaa tcggaatact
     2281 accgccagtt cctcgagccc gccgggatcc tctacatcct cggcgccgac gcgcgcgagc
     2341 cgctcggcat ggaggcgcgg ctgcgcgttt cgcgcgggcc cgacgacgcc ccgttcagca
     2401 aagacgacca gcgcctgctc gaacgcctgc tgccgcacct cgcgcgcgcg ctggagatca
     2461 acgcacggct ggcacgcacc gaatccgagc gcgcgctgta cgagggcgcc gtggaccagc
     2521 tcgcggtcgg caccatcatc ctcgacggcc atggccgggt gctgaacagc aaccgcgtgg
     2581 ccggcgagtt gctcgcgctg gccgacggca tgcgcgtgac cgactccgag ctgcgtttca
     2641 cggatccggc ggtcggcagg cagctgcagc aggtgatcga gaaggtgctg gatgcgcacc
     2701 tgcgcggcgc gccggcggtg gtcgaggcgc tgcgcgtgcg ccgaccttcc ggcaagggcg
     2761 atttcgggct ggtgatccgc ccggtgccgc gctcggcgtg gtccgagggg cagtcgagcc
     2821 ccaccgtcgc ggtgttcgtc agcgcgcccg aggtgcagtc acagacctcg gagctggtga
     2881 tcaagcagct gttcgatttc acgccggccg agtcgcgcct ggcgctgcgt ctcgccggcg
     2941 ggcaatccct ggccgaggcc tgcgaggagc tctgcatctc gcagcacacc gcgcgcgcgc
     3001 aactcaagac catcttcgcg aaatccggcg tcacgcggca ggccgagctg gtccgcctga
     3061 tcctgcgcag cgtggcgacg ctggcctgat gctcagcgga agatcttgat ccactcgccc
     3121 ggctgcggct cgccgcgcgg gtaatagccg ttcacgagcc gcagatgctc ctcggcgtga
     3181 tccttcagcg ggctggtctc ggccagcttc gcgaagctca tgccgggttc ggcgcggatg
     3241 taatgcaggt tgatgccgag caccgcatcg cggtcggcac cgctcatggg gcggaagctg
     3301 cggatcgcgg acaggaacat ggtgtcgtag aa
//
LOCUS       DAORUQ010000271         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_39130_length_3324_cov_5.404099, whole genome shotgun sequence.
ACCESSION   DAORUQ010000271 DAORUQ010000000
VERSION     DAORUQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39130_length_3324_cov_5.404099"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1105
                     /gene="purU"
                     /locus_tag="PLW13_15290"
     CDS             245..1105
                     /gene="purU"
                     /locus_tag="PLW13_15290"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HQY71792.1"
                     /translation="MHSQRCYTLSLSCPDRVGIVARVAGFFAGHQGWILESSQHADEQ
                     TERYFMRLEVRADSLPFHLAELRERFAPIARELDMEWRISDSAIRKRVVVLVSQQEHC
                     LYDLLGRWKSGELDIEIPCVISNHETWRGLVEWHGIPFHHVPVSAENRAQAFAEVERL
                     FVESRADTMVLARYMQILPPDLCRRHIGRIINIHHSFLPSFVGARPYHQAHERGVKLI
                     GATCHYVTEDLDQGPIIEQDLIRIDHSDSVEDLVRYGKDIEKAVLARGLRYHLEDRVL
                     VNGRRTVVFR"
     gene            1236..2039
                     /locus_tag="PLW13_15295"
     CDS             1236..2039
                     /locus_tag="PLW13_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQY71793.1"
                     /translation="MRVWFRYPLLVAAAVVLLAQPALAGKVLDRVVRTDTLRVGMSGD
                     QPPLNVIARDGATIGLEADLARLIAFGMGVELQIVNMPFAELEPALKKGKIDMIMSGM
                     SITPERARSMAFIGPYMMSGKSLVTRAGLLEEADETGDIDSPAVSIAVLGRSTSESFV
                     REYAPSAKVVAVANYEEGVRKLLDGEVAAMVADMPACAFALLRHPDAGLIALDEPLQI
                     EPIGIAVDARDAEFRNLLQNYVDVVRDSELGAGLSGKWFDDDEWLSLLP"
     gene            complement(2070..2438)
                     /locus_tag="PLW13_15300"
     CDS             complement(2070..2438)
                     /locus_tag="PLW13_15300"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY71794.1"
                     /translation="MANEHREKGIEMFNEVYCNDLPQPPAPGEDRFFDYMLDTVFGQL
                     WADETLGIRDRRLLLLGAIAALGEDMTFTIQARSALKRGELDRKQLREVVTFLTAYVG
                     YPRGAKLKLAVQTLLAQMEK"
     gene            complement(2476..2724)
                     /locus_tag="PLW13_15305"
     CDS             complement(2476..2724)
                     /locus_tag="PLW13_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQY71795.1"
                     /translation="MAAPEEEWIMGKLRVKVDLALCQGHAVCMQEAPEVFRVNETDSH
                     YPKVEVILEFPPEDLREKVMDAVRYCPNRTISVEEIPD"
     gene            2879..>3324
                     /locus_tag="PLW13_15310"
     CDS             2879..>3324
                     /locus_tag="PLW13_15310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY71796.1"
                     /translation="MAYRPQRMKFGAFFAPFHNDRTNPTLALESDMDLIACLDKLSFD
                     EAWIGEHHSGAYECIASPEIMIAAVAERTRHIRLGTGVSSLAYHHPLILADRIAQLDH
                     QTRGRVMFGVGPGQLIADAYMMGVNPADLRRRMNESLAALVKLLHG"
BASE COUNT          555 a         1101 c         1102 g          566 t
ORIGIN      
        1 caacagcgcg gcatcgccga tggggcgcta ctacccgggc gccggtgcga cgctgggatc
       61 cgcgatgacc tttgcgtaca tcgccgcgcg cgaggcgctg cgcacgtagg tcgcgcttca
      121 gcccgaccag ggttcggcaa agcccaaccc gccgcgttga acctcggtgc atgtcggctc
      181 tgaatgccga accaccacgc ggggcgggct atactgcgcg cgatcaatca ttcgcgagca
      241 ccgcatgcac tcccagcgct gttacaccct gagcctgtcc tgtcccgatc gtgtcggcat
      301 cgtggcgcgc gtcgccggat tcttcgccgg gcaccagggc tggatcctcg aatcgagtca
      361 gcacgccgac gagcagacag aacgctattt catgcgcctc gaggtgcgcg cggattcgct
      421 gccgtttcat ctggcggagc tgcgcgagcg cttcgcgccg atcgcgcggg agttggacat
      481 ggagtggcgc atcagcgaca gcgcgatcag gaaacgcgtg gtggtgctgg tgtcgcagca
      541 ggagcattgc ctctacgacc tgctcggacg ctggaagagc ggcgagctcg acatcgagat
      601 tccctgcgtg atctccaatc acgagacctg gcgcgggctg gtcgaatggc acggcatccc
      661 gtttcatcac gtgccggtga gcgcggaaaa ccgggcccag gccttcgccg aggtcgagcg
      721 gctgttcgtg gagtcgcgcg ccgacaccat ggtgctggcg cgctacatgc agatcctgcc
      781 cccggatctg tgccgccggc acatcgggcg catcatcaat atccaccaca gcttcctgcc
      841 gagcttcgtc ggcgccaggc cctatcacca ggcgcacgag cgcggcgtga agctgatcgg
      901 ggcgacctgt cactacgtga ccgaggacct ggaccagggc ccgatcatcg agcaggacct
      961 gatccgcatc gaccacagcg actcggtcga ggacctggtg cgctacggca aggacatcga
     1021 gaaggccgtg ctcgcgcgcg ggttgcgcta ccacctcgag gaccgcgtgc tggtcaacgg
     1081 gcggcgcacg gtggtgttcc gctagcggct tgacaggcgg atcgccgggc ccggagactc
     1141 ccggcacatc gcgccggggc cgctcgggag ccgggctcca tgcgcgcgaa ccggacgtcg
     1201 atccacgccg gccggcacca atcaggagta ttcccatgcg cgtatggttt cgttatccgc
     1261 tgctcgtcgc agcggccgtg gtcctgctgg cgcagccggc gctcgccggc aaggtgctcg
     1321 atcgcgtggt caggaccgat acactgcgcg tcggcatgtc gggcgaccag ccgccgctga
     1381 acgtgatcgc tcgcgacggc gccacgatcg gcctggaggc cgatctcgcc aggctgatcg
     1441 ccttcggcat gggggtcgag ttgcagatcg tgaacatgcc gttcgcggaa ctggaaccgg
     1501 cgctgaagaa aggcaagatc gacatgatca tgtccgggat gtcgataacc ccggaacgcg
     1561 cgcgcagcat ggcgttcatc gggccctaca tgatgtcggg caagtcgctg gtaacccgcg
     1621 ccgggctgct ggaggaagcc gacgagaccg gggacatcga cagcccggcc gtcagcattg
     1681 ccgtgctcgg caggtccacc agcgaaagct tcgtgcgcga gtacgcgccc agcgccaagg
     1741 tcgtggcggt cgccaattac gaggaggggg tgcgcaagct gctcgacggc gaggtggccg
     1801 cgatggtggc agacatgccg gcgtgcgcgt tcgcgctgct gcgtcacccc gacgccgggc
     1861 tgatcgcgct ggatgaaccg ctgcagatcg agccgatcgg catcgccgtt gacgcgcgcg
     1921 acgccgagtt ccgcaacctg ctgcagaact acgtcgacgt cgtgcgcgat tccgaactgg
     1981 gcgcgggcct gagcgggaag tggttcgacg acgacgaatg gctgtcgctg ctgccctgag
     2041 gcgctcgggc gcaactccgc ccgcgcgcgc tacttctcca tctgcgccag cagtgtctgc
     2101 acggccagct tgagcttcgc gccgcgcggg tagccgacgt aggcggtgag gaaggtcacg
     2161 acttcgcgga gctgcttgcg gtcgagctcg ccgcgcttca gtgccgagcg cgcctggatc
     2221 gtgaaggtca tgtcctcgcc gagtgctgcg atcgcgccga gcagcagcag gcggcggtcg
     2281 cggatgccga gcgtctcgtc ggcccacagc tggccgaaca ccgtgtcgag catgtagtcg
     2341 aagaagcggt cctcaccggg cgcgggcggc tgcgggaggt cgttgcagta gacctcgttg
     2401 aacatctcga tgcctttctc gcggtgctcg ttcgccatgt ctctctcctg cggttctcgt
     2461 ggtggtgaaa agggatcagt cggggatttc ttcgacgctg atggtccggt tgggacaata
     2521 gcgcacggca tccatcacct tttcccgcag gtcctccggc gggaattcga gtatcacctc
     2581 caccttgggg tagtggctgt cggtctcgtt gacgcggaac acctcgggtg cctcctgcat
     2641 gcagacggcg tggccctggc acagcgcgag atcgaccttg acgcgcaact tgcccatgat
     2701 ccattcctcc tcgggtgcgg ccatcgccgg atcgcgataa aacaattcga cgcaaaggtt
     2761 ttcagtctac gccggcgccc gctgcccggc aacaaaataa aaacattgct agtcaattgt
     2821 ttcctgatat gatgcctgca cgtccggcgt tccaccatcc agaaccgagg cccagaccat
     2881 ggcataccgc ccgcagcgca tgaagttcgg cgccttcttc gcgccattcc acaacgaccg
     2941 caccaatccc accctggcgc tcgagtccga catggacctg atcgcctgcc tggacaagct
     3001 ctccttcgac gaggcctgga tcggcgagca ccactcgggc gcctacgagt gcatcgcctc
     3061 gcccgagatc atgatcgccg cggttgccga gcgcaccagg cacatccgtc tcggcaccgg
     3121 ggtgagctcg ctcgcctacc accacccgct gatactcgcg gatcgcatcg cgcagctcga
     3181 ccaccagacg cgcgggcgcg tgatgttcgg cgtgggtccc gggcagctga tcgccgacgc
     3241 ctacatgatg ggcgtgaacc cggcggacct gcgccggcgc atgaacgagt cgctggccgc
     3301 gctggtgaag ctgctgcacg gcga
//
LOCUS       DAORUQ010000272         3321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_39181_length_3321_cov_6.597367, whole genome shotgun sequence.
ACCESSION   DAORUQ010000272 DAORUQ010000000
VERSION     DAORUQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39181_length_3321_cov_6.597367"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLW13_15315"
     CDS             <1..608
                     /locus_tag="PLW13_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71797.1"
                     /translation="FTNDYTDIQIAQFEAGTGGASSRIVNAGQGTYEGFEFDLVVVPV
                     DGLSIDATYGYLDAKFDEYLARNPATNQLVDISGNTTVAQAPENSASLGVQYDFEPFS
                     FGALSARVDVAYKDEYVFHPFQNLYDGADDRTLVNGRLSLNEIKMGCCDKGALRVSLW
                     GKNLTDEEYRNWSIDFGSLGWAGSVYGEPRTYGLDVVYSYE"
     gene            complement(702..1553)
                     /locus_tag="PLW13_15320"
     CDS             complement(702..1553)
                     /locus_tag="PLW13_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71798.1"
                     /translation="MPGTLRNKVAIITGASRGIGAAIALRFAAEGAKVVITARTVQPD
                     GKLPGSLEETAARIRALGAECLCVQADLGDPADRARIVPAAVAHFGGVDVLVNNAAWA
                     RFRPTVKQAPKDTRLAFEINYFAPLELSQQAIPSMAERGGGWILNLSSATSNHPAPAP
                     YDTANRYFQFHVHSGPTLYGSTKAALERMSAGLAAELAAQRIAVNALAPVEAVASEGA
                     LETGTIDAGAHMEPVEAMAEAALALCSRPPEQLSGRCVLSLPLLRELGVGVRTLDGRE
                     PLAGFTL"
     gene            complement(1606..2850)
                     /locus_tag="PLW13_15325"
     CDS             complement(1606..2850)
                     /locus_tag="PLW13_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71799.1"
                     /translation="MSGCPFANLIDPDTYAQGMPYAELKRYRDAGPVVQYDDPRTGVP
                     YWAIVGQHELDYVSKHPQLFSSAERSPFPSEYAQDMVENVHRKTIIGMDPPLHQKVRR
                     IMRAAFTPSAVDSYEPVFREHARRIVDAVLPRGECEFVEEVAAELPLLAILELLGVDP
                     ADRKDFFNWTNTMIFADDPDMSVSELDGQLASLKVIEYAMRLAAEHHRAPRKNVLGAL
                     LDGRIDGRPLSDEEFGWMFVLTLVGGNESTRTVIAHGMRLLMENPDQLQYLVDNPDRI
                     ADACEEILRYNTAFIAMRRTAMQDVELAGQTIRKGDKVLLHYHAINHDERVFGADAMR
                     FDVRRAERMPDLYNQLRSFGIGQHFCIGSHLARLELRVMFEEIIPRLRNPRLLAPPRY
                     VRSFFVNGVKEMRIRFDPVQVA"
     gene            3083..>3321
                     /locus_tag="PLW13_15330"
     CDS             3083..>3321
                     /locus_tag="PLW13_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQY71800.1"
                     /translation="MTDWLCGFALFAGIFCGDAGTTLSGYVEGETLFVAPIAVNRIAT
                     LAVRRGDEVKAGATLATMDADDAHFALEAARARLA"
BASE COUNT          534 a         1121 c         1135 g          531 t
ORIGIN      
        1 tcttcaccaa cgactacacc gacatccaga tcgcccagtt cgaagcgggc accggtggcg
       61 cgtcgagccg catcgtgaac gcgggccagg ggacctacga gggcttcgag ttcgacctcg
      121 tcgtggtacc ggtcgacggc ctctcgatcg acgcgaccta cggctacctc gacgccaagt
      181 tcgacgagta cctggcgcgc aacccggcga cgaaccagct cgtggacatc tcggggaaca
      241 ccacggtggc gcaggctccc gagaactccg cgtcgctggg cgtgcagtac gacttcgagc
      301 ccttcagctt cggtgcgctg tcggcgcgcg tggacgtggc ctacaaggac gagtacgtgt
      361 tccacccgtt ccagaacctc tacgacggcg ccgatgaccg cacgctggtc aacggacgcc
      421 tcagcctgaa cgagatcaag atgggctgct gcgacaaggg cgcgctgcgc gtgtcgctgt
      481 ggggcaagaa cctgaccgac gaggaatacc gcaactggag catcgacttc ggctcgctcg
      541 gctgggcggg ctccgtctat ggcgagccgc gcacctacgg gctcgacgtg gtctacagct
      601 acgaatgagc tgagcaacag ccgacgacga cagggcggcc tccgggccgc cctttttttg
      661 ccttcaattc atgcgagcca ggagagctcc cggcccgggg atcagagcgt gaatcccgcg
      721 agtggctcgc ggccgtccag cgtccgcacg ccaacgccca gctcccgcaa caacggcagg
      781 ctcagcacgc agcgcccgga gagctgctcc ggcggtcgcg agcagagcgc cagcgccgcc
      841 tccgccatcg cctccacggg ttccatgtgc gcaccggcgt cgatggtccc cgtctccagc
      901 gcgccttcgc tcgcgacggc ctccaccggc gccagcgcgt tcaccgcgat gcgctgcgcg
      961 gcgagttccg ccgccagccc cgccgacatg cgctccagcg cggccttggt ggaaccgtag
     1021 agcgtgggcc cgctgtgcac gtggaactgg aaatagcggt tcgcggtgtc gtagggcgcg
     1081 ggcgcgggat gattcgaggt ggcgctggag aggttgagga tccagccgcc accgcgctcg
     1141 gccatgctcg ggatcgcctg ctgcgagagc tcgagcggcg cgaaataatt gatctcgaag
     1201 gcgagccgcg tgtccttcgg cgcctgcttc acggtgggcc ggaagcgcgc ccacgcggcg
     1261 ttgttgacca gcacgtcgac gccgccgaag tgcgcgacgg ccgcgggcac gatgcgtgcg
     1321 cggtccgccg gatctcccag gtccgcctgc acgcagaggc attcggcacc gagcgcgcgg
     1381 atgcgcgccg cggtctcctc gagcgagccg ggcagcttgc cgtcaggctg cacggtgcgc
     1441 gcggtgatca ccaccttcgc gccctcggcc gcgaagcgca gcgcgatcgc cgcaccgatg
     1501 ccccggctcg cgccggtgat gatcgccacc ttgttcctca gggttccggg catcgtgtgt
     1561 tcctgtgatt gttcagcgat gacgacggcg acgcggcacg tgctcctagg cgacctgcac
     1621 cggatcgaag cggatgcgca tctccttgac gccgttgacg aagaacgagc gcacgtagcg
     1681 cggcggcgcg agcaggcgcg ggttgcgcag gcgcgggatg atctcctcga acatcacgcg
     1741 cagttcgagg cgcgccaggt gcgagccgat gcagaagtgc tgcccgatgc caaaggagcg
     1801 cagctggttg tagaggtccg gcatgcgctc ggcgcggcgc acatcgaagc gcatggcatc
     1861 cgcgccgaag acgcgctcgt cgtggttgat cgcgtggtag tgcagcagca ccttgtcgcc
     1921 cttgcgaatc gtctggcccg caagctccac gtcctgcatc gcggtgcgcc gcatcgcgat
     1981 gaaggcggtg ttgtagcgca ggatctcctc gcaggcgtcc gcgatccggt cggggttgtc
     2041 gacgaggtac tgcagctggt ccgggttctc catcagcaga cgcatgccgt gcgcgatcac
     2101 ggtgcgtgtg ctctcgttgc cgcccaccag cgtcagcacg aacatccagc cgaattcctc
     2161 gtcgctgagc ggccgaccgt cgatgcgccc gtcgagaagc gcacccagca cgttctttcg
     2221 cggcgcgcga tggtgctcgg cggcgagacg catcgcgtac tcgatcacct tcagcgaggc
     2281 aagctgcccg tcgagctccg acaccgacat gtcggggtca tcggcgaaga tcatcgtgtt
     2341 ggtccagttg aagaagtcct tgcgatcggc cgggtcgaca cccagcagct ccaggatcgc
     2401 cagcagcggc agctccgccg ccacctcctc gacgaactcg cactccccgc gcggcagcac
     2461 ggcatcgacg atgcgccggg cgtgctcgcg aaaaaccggc tcgtaggaat cgaccgcgct
     2521 cggcgtgaag gccgcgcgca tgatgcggcg cactttctgg tgcagcggcg gatccatgcc
     2581 gatgatggtc ttgcggtgga cgttctccac catgtcctgc gcgtactccg acgggaacgg
     2641 cgagcgctcc gccgaggaaa acagctgcgg gtgcttggag acgtagtcga gctcgtgctg
     2701 tcccacgatg gcccagtacg gcacgccggt gcgcggatcg tcgtactgga ccacgggccc
     2761 cgcatcgcga tagcgcttga gctcggcata gggcatgccc tgggcgtagg tgtccgggtc
     2821 gatcaggttg gcgaacgggc aaccactcac ggctttctcc aacggtgcac taatgcccgt
     2881 tagtctagcc acggccgttg cgcgacgtcc agcgcgcggc gcgcccggcg gattacgcgc
     2941 gcgtaatctg cgcgccgccc gctgcacatg cgacacaaat accgcgggcc ggcgtatttg
     3001 ccgcccagca catacaattg agacgcgagt tgccgagggg tcgggtagcg gcagccgcgg
     3061 agcatgtttc gaggaggatc ggatgacgga ttggctgtgc ggcttcgcgc tgttcgccgg
     3121 tatcttttgc ggcgatgcgg gcaccacgct cagcggttac gtcgagggag agacgctgtt
     3181 cgtggcgccg atcgcggtga accgcatcgc cacgctcgcc gtgcgccgcg gcgacgaggt
     3241 gaaggccggc gcgactctgg ccacgatgga cgccgatgac gcccacttcg cgctggaggc
     3301 cgcgcgcgcg cggctcgccg a
//
LOCUS       DAORUQ010000273         3298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_39645_length_3298_cov_6.494604, whole genome shotgun sequence.
ACCESSION   DAORUQ010000273 DAORUQ010000000
VERSION     DAORUQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39645_length_3298_cov_6.494604"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLW13_15335"
     CDS             complement(<1..851)
                     /locus_tag="PLW13_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HQY71801.1"
                     /translation="MSRTPLVSGSEWTFDLIDECYGHIARIAAQFGLDTYPNQIEVIT
                     SEQMLDAYARIGMPIGYHHWSFGKHFIINEQQYKRGQMGLAYEMVINSNPCIAYLMEE
                     NSMMMQCLVMAHACFGHNSFFKSNYLFRTWTDAEAIIDYLVFARNYLAECEQRHGELE
                     VELLLDSCHALMNHGVDRYKRPNPIGLREERRRQEEREAFLQSQVNDLWRTIPHGRDR
                     TAARAAPRFPPEPQENILYFIEKNAPLLQPWQREVVRIVRKLGQYFYPQRQTQVMNEG
                     WATFWHYT"
     gene            complement(848..2104)
                     /locus_tag="PLW13_15340"
     CDS             complement(848..2104)
                     /locus_tag="PLW13_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HQY71802.1"
                     /translation="MPHLIDRRQWGKKRSTVNRQRFIRRFKDQIKRALAEQIDRRGVT
                     DVQNGESVSIPARDTGEPTFRQGAGGHRERIFPGNHEYVSGDRIPKPQGGGGGQGQGR
                     RAGDSGEGEDEFRFEISRDEYLDLMFEDLELPRLDITQLKNVERFRNVRAGYRTDGVP
                     TNLNIVRSLERALARRIALSGGDRRELRELEAELAQPGDDAQGARVALRARIEELRER
                     IARVPFIDTFDLRFTAFAKQPMPSTQAVMFCLMDVSGSMDQQRKDIAKRFFLLLYLFL
                     SKSYANVQVVFIRHHTQAKEVSEQEFFHSQESGGTVVSSALRLLDETIRERFSPELWN
                     VYVAQASDGDNWHDDSPICQDLLANRILPSVRYFSYIEIAGQPQGLWHHYEALLAAHP
                     NLATRRILQAEDIYPVFRELFRKQHA"
     gene            complement(2144..>3298)
                     /locus_tag="PLW13_15345"
     CDS             complement(2144..>3298)
                     /locus_tag="PLW13_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HQY71803.1"
                     /translation="FVEMFKAPIKVLHPLLTATQEGNYNGTEGLSALPFSGIILAHSN
                     ESEWVGFRNNRNNEAFLDRVFIVKVPYCLRVSEEIKIYRKLLAGSDLHAAPCAPGTLE
                     MMAQFSVLSRIQEPGNSSVYSKMRVYDGESLKDTDPKAKSYQEYRDAAGVDEGMNGLS
                     TRFAFKILSRVFNFDATEIAANPVHLLYVLETQLEREQFPQDVHDRYLEHLKNFLVPR
                     YVEFIGKEVQTAYLESYSEYGQNLFDRYVLYADFWIQDQEYRDPETGENFDRAALNEE
                     LEKIEKPAGISNPKDFRNEVVNFVLRARASNQGHNPVWTSYEKLRSVIEHKMFSTTED
                     LLPVISFNAKASKEDQQKHQDFVVRMVKKGYTEKQVRLLSEWYLRVRKAS"
BASE COUNT          506 a         1031 c         1116 g          645 t
ORIGIN      
        1 gtgtagtgcc agaacgtggc ccagccctcg ttcatgacct gggtctggcg ctgcgggtag
       61 aagtactgac cgagcttgcg cacgatgcgc acgacctcgc gctgccacgg ctggagcagc
      121 ggcgcgttct tctcgatgaa atagaggatg ttctcctgcg gctcgggcgg gaatcgcggc
      181 gcggcgcgcg cggccgtcct gtcgcgcccg tgcgggatcg tgcgccacag gtcgttgacc
      241 tgcgactgca ggaaggcctc gcgctcctcc tggcggcgcc gttcctcgcg cagcccgatc
      301 gggtttggcc gcttgtagcg gtccaccccg tggttcatca gcgcgtggca ggaatccagc
      361 agcagctcga cctcgagctc gccgtggcgc tgttcgcact cggcgaggta gttacgcgcg
      421 aaaaccaggt agtcgatgat ggcctcggca tcggtccagg tgcggaacag gtagttcgac
      481 ttgaagaacg agttgtggcc gaaacaggcg tgcgccataa ccaggcactg catcatcatg
      541 ctgttctctt ccatgagata cgcgatgcag gggttggagt tgatcaccat ctcgtaggcg
      601 agacccatct ggccgcgctt gtactgttgc tcgttgatga tgaagtgctt gccgaaggac
      661 cagtggtggt agccgatcgg catgccgatg cgcgcgtagg cgtcgagcat ctgctccgac
      721 gtgatcacct cgatctggtt ggggtaggtg tcgaggccga attgcgccgc gatccgcgcg
      781 atgtgcccgt agcactcgtc gatcagatcg aaagtccact ccgaaccgct gaccaggggc
      841 gtgcggctca tgcgtgctgc ttccggaaca gttcgcggaa caccgggtag atgtcctcgg
      901 cctgcaggat ccgccgcgtc gcgagattgg ggtgcgcggc gagcagcgcc tcgtagtgat
      961 gccacagccc ctgcggctgc ccggcgatct cgatgtagga gaagtagcgc acgctgggca
     1021 ggatgcggtt ggcgagcagg tcctggcaga tcggcgagtc gtcgtgccag ttgtcgccgt
     1081 cggaggcctg cgccacgtag acgttccaga gctcgggcga gaagcgctcg cggatggttt
     1141 cgtcgagcag gcgcagcgcg ctggagacca cggttccgcc gctctcctgg gagtggaaga
     1201 attcctgctc gctgacttcc ttcgcctggg tatggtggcg gatgaacacc acctgcacgt
     1261 tcgcgtagct cttgctgagg aacaggtaga gcagcaggaa gaagcgcttg gcgatgtcct
     1321 tgcgctgctg gtccatcgag cccgagacat ccatcaggca gaacatcacg gcctgggtgg
     1381 acggcatggg ctgcttcgcg aaggcggtga agcgcaggtc gaaggtgtcg atgaacggca
     1441 ccctggcgat gcgctcgcgc agctcctcga tgcgcgccct gagcgcgacg cgcgccccct
     1501 gcgcgtcgtc gccgggctgc gcgagctcgg cttccagttc gcgcagttcg cggcgatcac
     1561 cgccgctgag cgcgatgcgc cgcgccagcg cgcgctcgag cgagcgcacg atgttcaggt
     1621 tcgtcggcac gccgtcggtg cggtaaccgg cgcggacgtt cctgaaacgc tcgacgttct
     1681 tcagctgcgt gatgtcgagg cgcggcagtt cgagatcctc gaacatcagg tcgaggtact
     1741 cgtcgcgcga gatctcgaag cggaactcgt cctcgccctc gcccgagtcc ccggcgcgtc
     1801 gcccctggcc ctgcccgccg ccgccaccct ggggtttggg gatgcggtcg ccgctcacgt
     1861 actcgtggtt gccggggaat atccgctcgc gatgcccgcc cgcgccctgg cggaaggtgg
     1921 gctcgccggt gtcgcgcgcc gggatgctca cgctctcgcc gttctgcacg tccgtgacgc
     1981 cgcgtcggtc gatctgctcg gcgagcgcgc gcttgatctg gtccttgaag cggcggatga
     2041 agcgctggcg gttgaccgtg ctgcgcttct tcccccactg gcgccggtcg atcaggtgtg
     2101 gcatgccgcg gcgctccgtt ccatgcgtgt cgatgggttc gcgtcagctc gccttgcgca
     2161 cccgcaggta ccactccgag agcaggcgca cctgcttctc ggtgtagccc ttcttcacca
     2221 tgcgcaccac gaaatcctgg tgcttctgct ggtcctcctt cgaggccttg gcgttgaagg
     2281 aaatcaccgg cagcaggtcc tcggtggtcg agaacatctt gtgctcgatg acggagcgca
     2341 gcttctcgta gctggtccac accgggttgt gcccctggtt ggaggcgcgc gcgcgcagca
     2401 cgaagttcac gacctcgttg cggaaatcct tcggattgct gatgccggcg ggcttctcga
     2461 tcttctcgag ctcctcgttc agcgccgcgc gatcgaagtt ctcgcccgtt tccggatcgc
     2521 ggtactcctg gtcctggatc cagaagtcgg cgtacagcac gtagcggtcg aacaggttct
     2581 ggccgtactc ggaataggac tcgagatacg ccgtctggac ttccttgccg atgaactcga
     2641 cgtagcgcgg cacgaggaag ttcttgaggt gctcgaggta acggtcgtgc acgtcctgcg
     2701 ggaactgctc gcgctcgagc tgggtctcca gcacgtacag caggtgcacc gggttggcgg
     2761 cgatctcggt ggcgtcgaag ttgaacacgc gcgagaggat cttgaacgcg aaacgcgtcg
     2821 agaggccgtt catgccctcg tccacgccgg cggcgtcgcg gtactcctgg taggacttcg
     2881 ccttcgggtc ggtgtccttc agcgactcgc cgtcgtagac gcgcatcttg gagtagacgc
     2941 tggagttccc gggctcctgg atgcgcgaga gcaccgagaa ctgcgccatc atctccagcg
     3001 tgcccggcgc gcagggcgcc gcgtgcaggt cgctgccagc cagcagcttg cggtagatct
     3061 tgatttcctc gctgacgcgc aggcagtagg gcaccttcac gatgaacacg cggtcgagga
     3121 aggcctcgtt gttgcggttg ttcctgaaac cgacccattc cgattcgttc gagtgcgcga
     3181 ggatgatgcc gctgaagggc agcgccgaga ggccctcggt gccgttgtag ttgccctcct
     3241 gggtcgccgt cagcagcgga tgcagtacct tgatcggcgc cttgaacatc tcgacgaa
//
LOCUS       DAORUQ010000274         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_39722_length_3294_cov_7.745909, whole genome shotgun sequence.
ACCESSION   DAORUQ010000274 DAORUQ010000000
VERSION     DAORUQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39722_length_3294_cov_7.745909"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>3294)
                     /gene="traA"
                     /locus_tag="PLW13_15350"
     CDS             complement(158..>3294)
                     /gene="traA"
                     /locus_tag="PLW13_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HQY71804.1"
                     /translation="TRQVTAEGLGDKTYLERENKWLLANGLPTTDMQLRDLRQTWEGI
                     ANEHLARAGHDLRIDHRSHMERGLEITPTEHMGVHATQMERRGMDVARTRLDEDAARR
                     NADLIREKPEQVLSLITAEKSVFDRHDVARALHRYINDDAQEFQSAFAKVMASPALAE
                     LQPERTDPETGEVKLARYSTREMVEIESGMMASAQRMSAARDHGVDRRHVDRAIGAQD
                     AAIQRSAGDASARLSDEQRRAIEHITGPEGISAVVGFAGAGKSTMLAAAREAWEAEGY
                     RVHGAALSGKAAEGLEESSGIQSRTLASWSRGWENERGQLGRGDVFVIDEAGMIGSRQ
                     LARFVGEAEARGAKIVLVGDHEQLQAIGAGAPFRAITEEIGHAELSEVRRQRVDWQRQ
                     ASVDFATHRTPEGLAAYRERGDIRFSADRDGARGEIVRDYLADHDARPDGSRVAMAHR
                     RADVRAINEAIRAELQERGELARVSVPCDGEDGASGARVFQTNDGQREFAPGDRIVFL
                     ENNRDLGVKNGMLGTVERVEEGRIIAALDGKGDSISVPMGDYQAIDHGYATTIHKNQG
                     ATVDRAFVMASGTMDRHLTYVAMTRHRDGAQLYAAQDDFTDRHAGRLVEHGAAPFEHK
                     DGNRPSYFVTLENDKGERLTTWGVDLERAMTEAKPEIGEKIGLQHIGATPVTLPDGTE
                     TQRNAWKVRDAGDLAYEQLERRLSRSGAKETTLDYTRDFAERRGIAESLGIRSEIEVP
                     REGLSRDGAEQRPEREAPAERARIEDSPARSMSEIHKDLAQDLFAYAGLDHGEDQGAG
                     RAERQTEKPAPRRRSMFDGLKLNAASRAPVEPGRPEREGGRKVSAPAPDRLAARLRAP
                     TPLEAAVDRYARAYASADQHLRDGLPILDMQRQELRAAGQQLDQVKGGMRNLMLSTLQ
                     HDPKTEQAITDLSGRERVARVIEGMTRENAALADPHVRAERFVTRWRALQEQRRELYG
                     RYDDEARGKVDSQLNGMAQSLERDPQVDSLLRNRRQELGLRQEPQREKPVARELQDDM
                     ERGHQISRGHGLGL"
BASE COUNT          483 a         1246 c          965 g          600 t
ORIGIN      
        1 tcctgcgccg cagcgcgtcg aacgcctcgg ccggatcgcc agaatctgcc tccggttctg
       61 cctcgccctc gtcccattgc cccggctttt ccggctcaac ctcttcgaca ctgccgcccc
      121 ggaccctcat acgcaccatc cccaccaact ccttacttta caggccaaga ccatgccccc
      181 ggctgatctg gtggccgcgc tccatgtcgt cttgcagctc gcgggcgact ggcttctcgc
      241 gctgcggctc ctgccggagt ccaagctcct gtcggcgatt gcgcagaagg gaatccacct
      301 gcggatcgcg ttcaagactt tgggccatgc cgttcaactg gctatcgacc ttgccacgcg
      361 cctcgtcgtc ataccgaccg taaagctccc ggcgctgctc ttgcaacgcc cgccagcggg
      421 taacaaaccg ctcggccctg acatggggat cggccagtgc ggcgttctcg cgcgtcatgc
      481 cctcgatgac acgggcgaca cgctcccggc cggatagatc ggtgatggcc tgttcggttt
      541 tcggatcatg ttgcagggtc gaaagcatca ggtttctcat gccgcccttg acctgatcga
      601 gctgctgccc ggccgcccga agctcctgcc tctgcatgtc gagaattggc aggccatcgc
      661 gcaggtgctg gtccgccgag gcataggccc gcgcatagcg atcaacggcc gcctcaagcg
      721 gcgtcggcgc ccgcaggcgc gcggccagcc ggtcgggcgc tggcgccgac accttccgcc
      781 ccccctccct ttccggccgc cccggctcca caggcgcgcg ggaagcggca ttgagcttga
      841 ggccgtcgaa catgctgcgc cgacgcggcg cgggtttctc ggtctggcgc tcggcccgcc
      901 ctgcaccttg atcctcgcca tgatcgaggc cggcatacgc gaaaagatcc tgtgcaagat
      961 ctttgtggat ctccgacatg gatctcgccg ggctgtcctc gatccgcgcc cgctcggccg
     1021 gggcctccct ctccgggcgc tgctctgcac cgtcacggga aagcccctcg cgcgggacct
     1081 cgatctcgct gcggatgcca aggctttccg cgatcccgcg ccgttcggca aaatcgcggg
     1141 tataatccag cgtggtttcc ttcgcgccag accgcgacaa ccgccgctct agctgctcat
     1201 aggccagatc gccggcgtcc cgcaccttcc aggcattgcg ctgcgtctcg gtgccatcgg
     1261 gcagggtgac gggggtagcg ccgatatgtt gcaggccgat cttctccccg atctccggct
     1321 tggcctcggt catggcgcgt tccaggtcca cgccccatgt ggtgagccgc tcgcccttgt
     1381 cgttttccag ggtcacgaaa taactcggcc ggttgccgtc cttatgctcg aagggcgcag
     1441 ccccatgctc gaccaggcgg ccggcgtgac ggtcggtaaa atcatcctgc gccgcataga
     1501 gctgcgcgcc gtcccgatgc cgggtcatgg caacataggt caggtgccgg tccatcgtgc
     1561 ccgaggccat cacaaaggcc cgatccaccg tcgccccttg gttcttgtgg atcgtggtcg
     1621 catagccgtg gtcgatggcc tgataatctc ccatcggaac gctgatgctg tcacccttac
     1681 cgtccagcgc cgcgatgatc ctgccttcct cgacccgctc caccgtgcca agcatcccgt
     1741 tcttcacccc gaggtcgcgg ttgttctcaa ggaacacgat ccggtcgccc ggcgcgaact
     1801 cgcgctgccc gtcattggtc tggaacaccc gtgcgccgct ggcaccgtcc tcgccatcgc
     1861 agggcacact gacccgcgcc agctcgcccc gctcctgtag ctcggcccgg atcgcctcat
     1921 tgatcgcccg cacgtccgcc cggcgatgcg ccatcgcaac ccggctgcca tccgggcgcg
     1981 catcgtggtc ggcaaggtag tcgcgcacga tctcgccgcg cgcgccgtcg cggtcggcgc
     2041 tgaaccgaat atccccccgc tcccgatagg ccgccagccc ctcgggcgtg cgatgcgtgg
     2101 cgaagtcaac cgacgcctgc cgctgccagt ccacccgctg ccggcggact tcggacaact
     2161 cggcatggcc gatttcctcg gtgatcgccc ggaacggtgc ccccgccccg atggcctgta
     2221 gctgctcgtg atcgcccaca aggacgatct tcgcgccgcg cgcctcggcc tcgcccacga
     2281 accgggcgag ctggcgcgac ccgatcatgc ccgcctcgtc gatcacgaac acgtcgccgc
     2341 ggccaagctg gccccgctcg ttctcccaac cgcgggacca ggacgcgagc gtgcggctct
     2401 ggataccgga cgattcctcc aacccctcgg ccgccttccc cgacaaggca gcaccatgca
     2461 cccgatagcc ctcagcctcc cacgcctccc gggcggccgc cagcatcgtg gacttgccag
     2521 cgccggcgaa cccgaccacc gcagaaatcc cctccggccc ggtgatatgc tcgatggcgc
     2581 ggcgctgctc atccgacagc cgggccgacg catcgccggc gctgcgctgg atcgcggcat
     2641 cctgcgcccc aatggcccgg tctacatgcc ggcgatcaac accatgatcc cgcgccgcac
     2701 tcatccgctg cgcgcttgcc atcatgccgg attcgatctc gaccatttcc cgggtggaat
     2761 agcgggcaag cttgacctcg cccgtctccg ggtctgtgcg ctccggctga agctcggcca
     2821 gcgccggcga agccatcacc ttggcaaagg cgctctggaa ctcctgcgca tcgtcattga
     2881 tgtagcgatg cagcgcccgc gcaacgtcat gtcggtcgaa cacgcttttc tcggccgtga
     2941 tgagggaaag cacctgctcc ggcttctcgc ggatcagatc ggcattgcgc cgcgccgcat
     3001 cctcatcgag ccgcgtgcgc gccacgtcca tgccgcgccg ctccatctgc gtggcatgga
     3061 cccccatatg ctcggtcggc gtgatctcta gtccgcgctc catatgggaa cggtggtcga
     3121 tccgcagatc atgtccggcc cgcgccagat gctcgttggc gatgccttcc caagtctggc
     3181 gcagatcgcg aagctgcata tcggtcgtgg gcaagccgtt cgccaacagc catttgttct
     3241 cgcgctcaag ataggtcttg tcaccaagcc cctccgccgt cacctgccgc gtcg
//
LOCUS       DAORUQ010000275         3286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_39891_length_3286_cov_5.510059, whole genome shotgun sequence.
ACCESSION   DAORUQ010000275 DAORUQ010000000
VERSION     DAORUQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39891_length_3286_cov_5.510059"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLW13_15355"
     CDS             complement(<1..980)
                     /locus_tag="PLW13_15355"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HQY71805.1"
                     /translation="MKQKPLSFALTALALLGLGAQQAGADIVLLKGGDRLSGTIVSKD
                     DKQLTLQTAYAKEPITLLWAEVTNIETDQPARFMLNDKTLVNAQASRASESTVVLKGG
                     DTVTTTPLAMSDIAYINPPPHVSGEGVSTSGRLNLGMTANRGNTDNDQIFYDAEAVAR
                     SLKNRFTIGATGDTRNEDGEETARRNRAYFKYDHFLTDKWYAYANTDVQEDKFRDLNL
                     RTTLGGGAGYQFFDTEQRSLSLEGGLTYVNEDYDLADDESFAAGRWAVRYMQFLWNDR
                     AQFFHDDEGIVSVQDPDDMIFRSQTGLRFPLVKNLNATLQYNIDWQNTPP"
     gene            1145..1441
                     /locus_tag="PLW13_15360"
     CDS             1145..1441
                     /locus_tag="PLW13_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY71806.1"
                     /translation="MSPPVSKQRKAYCRKLCVAHLLAREPHDLHALEAATGMPRRTLQ
                     DCIADLADIGIVCEFVQNGPKHRHGHYAIRDWGDHDPRWIAAHFDNLLAVLRAE"
     gene            1438..3117
                     /locus_tag="PLW13_15365"
     CDS             1438..3117
                     /locus_tag="PLW13_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY71807.1"
                     /translation="MSGRLERIAPGLLMLARYRRQDFPHDLRAGLSVAAVALPVGVAY
                     AALAGFSPVVGLYASILPLFAYALFGSSRQLVVGPDAATCALVAASVAPLAAGDAQQY
                     MALSLTLAFLAGVLCIGASFLKLGVLADFLSRPILVGFMNGIALTIALGQLGKILGFD
                     IVSGRIIPRALEIADKIGQTHLPTLAIGLASFALLLLVPKLLRRVPAALVVMVLAAAA
                     VALLGLDARGVRTVGAVPPGLPALTFPGVPLSALQPLLADAAGIALISFTSLMLTARS
                     FAAKNGYEIDADRDLAALGAANIAASISGGFAVSGADSRTAMNDAAGGRTQLAGLVAA
                     AAIALVLLFLTGPLQYVPDAALGAVLVVAAFSLVNVADMRMLWHESKGEFAICVIATL
                     GVVAVGAIDAIAFAVILAMLRFVRMVARPTCEVLGAIEGRGFHGIERHPEARTVPGLC
                     LFRFNAPLVFFNAPYFRQEARRAVAAAGRDLRFFVIDAIPITGHDVTGRATFRELDQE
                     LLAQGVQIVIAGRETELMEWRERTGMDHLRPIKVRQFPTLADAVQALQAQL"
BASE COUNT          439 a         1078 c         1150 g          619 t
ORIGIN      
        1 tccggcgggg tgttctgcca gtcgatgttg tactgcagcg tcgcgttcag gttcttcacc
       61 agcgggaagc gcaggccggt ctgggagcgg aagatcatgt catccggatc ctggacgctg
      121 acgatgccct cgtcgtcgtg gaagaactgg gccctgtcgt tccacaggaa ctgcatgtag
      181 cgcaccgccc agcgtccggc cgcgaagctc tcgtcgtccg cgaggtcgta gtcctcgttg
      241 acgtaggtga gaccgccttc cagtgagagg ctgcgctgct cggtgtcgaa gaactggtaa
      301 cccgcaccgc cgccgagcgt ggtgcgcagg ttgaggtccc tgaacttgtc ctcctgcacg
      361 tcggtgttgg cgtaggcgta ccacttgtcg gtcaggaaat gatcgtactt gaagtaggcg
      421 cggttgcggc gcgctgtctc ctcgccgtcc tcgttcctgg tatcgccggt cgcgccgatc
      481 gtgaagcggt tcttcaggct gcgcgccacg gcctcggcgt cgtagaagat ctggtcgttg
      541 tcggtgttgc cgcggttggc ggtcatcccg agattcaggc gtccgctggt gctcacgccc
      601 tcgccgctga cgtgtggcgg cgggttgata taggcgatgt cgctcatcgc cagcggcgta
      661 gtggtgacgg tatcgccccc cttcaatacc acggtggact cgctggcgcg cgaggcctgc
      721 gcgttcacca gcgtcttgtc gttgagcatg aagcgcgcgg gctggtcggt ctcgatgttc
      781 gtgacctcgg cccacagcag cgttatcggc tccttggcgt aggcggtctg caacgtcagc
      841 tgcttgtcgt ccttgctgac gatggtgccg ctcaggcggt cgccgccctt caggaggacg
      901 atgtcggcgc cggcctgctg cgcgccgagg cccagcaggg ccagcgctgt cagtgcgaag
      961 gaaagcggtt tttgcttcat gttcaggttc cctgtcgttg gttgcttgcg tgacgcgccg
     1021 gagtataacg gccgggtcgg cggaatacag caacgcccgg ttcgggggtc ttttcccggc
     1081 gccgcggata tggcccaatc ggcatcacga cagcgccacg accctccagt ggagaacccc
     1141 gcccatgagc ccgccggtca gcaagcagcg caaggcctac tgccgcaagc tctgcgtggc
     1201 gcacctgctc gcgcgcgagc cgcatgacct gcatgcgctg gaggcggcca ccggcatgcc
     1261 gcggcgcacg ctgcaggact gcatcgcgga tctcgccgac atcggcatcg tgtgcgagtt
     1321 cgtgcagaac ggaccgaagc atcgccacgg tcactacgcg atccgcgact ggggcgatca
     1381 cgatccgcgc tggatcgcgg cgcatttcga caacctgctc gcagtgctgc gcgcagaatg
     1441 agcggccgac tggagcgcat cgcgccgggc ctgctgatgc tggcgcgtta ccggcggcag
     1501 gattttccgc atgacctgcg cgccggcctg tcggtggccg cggtggcgct gccggtcggc
     1561 gttgcctatg ccgcgctcgc cggtttcagc ccggtggtcg ggctctacgc gagcatcctg
     1621 cccctgttcg cctacgcgct gttcggctcc tcgcgccagc tcgtggtcgg accggacgcg
     1681 gccacctgcg cgctggtggc ggcctcggtg gcgccgctgg ccgcgggtga tgcgcagcag
     1741 tacatggcgc tgtcgctgac gctggctttc ctggcgggcg tgctgtgcat cggcgcgagc
     1801 ttcctgaaac tcggcgtgct ggccgatttc ctgtcgcgcc cgatcctggt cggattcatg
     1861 aacggcatcg cgctgaccat cgcgctgggg caactgggca agatcctcgg cttcgacatc
     1921 gtgtcgggca ggatcattcc gcgcgcgctg gagatcgcgg acaagatcgg gcagacgcac
     1981 ctgcccacgc tggccatcgg cctcgcttcc ttcgcgttgc tgctgctcgt gccgaagctg
     2041 ttgcggcgcg tgccggccgc gctggtggtc atggtgctcg ccgcagcggc cgtcgcgctg
     2101 ctggggctgg atgcacgggg cgtgcgtacc gtcggcgcgg tgcccccggg ccttcccgcg
     2161 ctgacttttc ccggcgtgcc gctgtcggcg ctgcaaccgc tgctggccga cgcggcgggc
     2221 atcgcgctga tctccttcac cagcctcatg ctcaccgcgc gcagctttgc cgcgaagaac
     2281 ggctacgaga tcgatgccga ccgcgacctc gcggcgctcg gtgcggccaa catcgctgcc
     2341 tcgatatcgg gcggcttcgc ggtgagcggc gccgactcgc gcacggccat gaacgatgcc
     2401 gccggcgggc gcacgcagct cgccggactg gtcgctgcgg ccgcgatcgc gctggtgctg
     2461 ctgttcctga ccgggccgct gcagtatgtg cccgacgccg cgctgggcgc ggtgctggtg
     2521 gtggcggcat tctcgctggt caacgtcgcc gacatgcgga tgctgtggca cgagtcgaag
     2581 ggcgagttcg cgatctgcgt gatcgcgacg ctcggtgtgg tcgccgtcgg cgcgatcgac
     2641 gcgattgcgt tcgcggtgat cctcgccatg ctgcgtttcg tgcgcatggt ggcgcgcccg
     2701 acctgcgagg tgctgggcgc catcgagggc aggggattcc acggcatcga gcgccacccc
     2761 gaggcgcgca ccgtgccggg actgtgcctg tttcgcttca acgccccgct cgtgttcttc
     2821 aacgcgccct atttcaggca ggaagcgcgg cgcgccgtgg cagcggcggg gcgggatttg
     2881 cgtttcttcg tgatcgacgc catcccgatc acgggccacg acgtgacggg acgcgccacg
     2941 tttcgcgagc tggaccagga actgctggcg cagggtgtgc agatcgtgat cgccgggcgc
     3001 gaaacggagc tcatggagtg gcgcgagagg accggcatgg atcatctgcg ccccatcaag
     3061 gtgcgccagt tcccgacgct ggccgacgcg gtgcaggcgc tgcaggcgca gctgtagagg
     3121 gtgtcgcaaa accatctgtg atcgggataa cgagcccgac aatcccgcgt atcgcgagtc
     3181 atcggcgccc gagcacccat cggtgccgcg gacacgccgt aaatacatcc gtgtaggcta
     3241 cctgtcgccg tcctggcgac agagtgtccg ccgcaccgat gggtgc
//
LOCUS       DAORUQ010000276         3275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_40100_length_3275_cov_4.738509, whole genome shotgun sequence.
ACCESSION   DAORUQ010000276 DAORUQ010000000
VERSION     DAORUQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40100_length_3275_cov_4.738509"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLW13_15370"
     CDS             complement(<1..208)
                     /locus_tag="PLW13_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71808.1"
                     /translation="MTVRAHCSRWMSAAVFSLAALGGAAQADSALDSALAGEHRSEAN
                     RARDAYRHPVETLGFFGIAPDMTVV"
     gene            333..857
                     /locus_tag="PLW13_15375"
     CDS             333..857
                     /locus_tag="PLW13_15375"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQY71809.1"
                     /translation="MQTYLLISFIADDRPGLVQSLSEAVAAHGGNWLESRMANLAGKF
                     AGIVNVSVPQARRQDLEGALGALEAQGLTLRLEAARAAAPAPAHTPWRLDIVGQDRPG
                     IVHEFSAALASRRINVVELRSDITSAPMSADPLFVASVGIHVPADIDLDELRERLEAI
                     ADELTIDYALVAAD"
     gene            complement(879..1100)
                     /locus_tag="PLW13_15380"
     CDS             complement(879..1100)
                     /locus_tag="PLW13_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71810.1"
                     /translation="MNLFALFRSNRMLVFGIVATLALLVSAAMAFDFRLAAILGFLWQ
                     CLLLIVAMILTAACAVALLVGARALWRRR"
     gene            complement(1097..1606)
                     /locus_tag="PLW13_15385"
     CDS             complement(1097..1606)
                     /locus_tag="PLW13_15385"
                     /inference="COORDINATES: protein motif:HMM:NF026153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY71811.1"
                     /translation="MTMNFCALCGAPLTTGVPASDDRERPACSACARVYYRNPEVHVG
                     CIVCQPDGHTPALLLAQAGRGEKIQSAVLRALEPALTGASPREEDLVLYATLTDAGSE
                     HLFLVFRAPGGCLEAAAQGVAQAPWAPELLAHFARERITHRFDVYTGHHDGVRLHLRA
                     VPTDRGIDA"
     gene            complement(1623..2810)
                     /locus_tag="PLW13_15390"
     CDS             complement(1623..2810)
                     /locus_tag="PLW13_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71812.1"
                     /translation="MGAPVAGIPYWRLSSFYFLYYAFLGAMMPYWSLYLQAVGLDAAA
                     IGVVLAVMAAIRIVAPNVWGWLADRSGRRLLIIRTGALAALITFAGLLVRTDLWWLVA
                     VVALHSVFWNAILAQYEVITLASLGEQVHRYGQVRLWGSVSFIVAVVGAGQLFDRLSV
                     TWLPQLLLALLVLIAAGTLLIREPAVARREPPAGGLRELLGARPVQAFLAASFLLQFS
                     HAPYYGFFSLYLEQHGYSRAATGLIWSLGVIAEIGVFACAHRLLARFGLRAILLASLL
                     LAAVRWLMIGFGVDSLALLLVAQCLHAGSFGSFHAAGIEFLRRYFGAAQQGQGQAVYA
                     AVSFGAGGALGALLSGLLWDMSARLGFVVAALAALAGWWIVRAAVRGPLVEAPATARA
                     IGQ"
     gene            complement(2810..>3275)
                     /locus_tag="PLW13_15395"
     CDS             complement(2810..>3275)
                     /locus_tag="PLW13_15395"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQY71813.1"
                     /translation="GLGEPVFDRLDADIAHAMMGINAVKGVEIGAGFAAVAQKGTEHR
                     DEITPAGFLSNHSGGILGGISSGQDIVVSIALKPTSSMRLPGRSIDRFGQPIEVVTHG
                     RHDPCVGIRATPIAEAMLALVLMDHWLRHRAQNADVRCETPVVPGSVVAPTG"
BASE COUNT          554 a         1186 c         1143 g          392 t
ORIGIN      
        1 cgaccacggt catgtcgggc gcgatgccga agaagccgag cgtctccacc gggtggcgat
       61 aagcgtcgcg cgcgcggttg gcctcgctgc ggtgctcgcc ggcgagcgcg ctatcgagcg
      121 cgctgtcggc ctgcgccgcg ccaccgagcg cggcgagcga aaacacggct gcggacatcc
      181 atcgggagca gtgggcacga acagtcatgg cgtggttcct cggtcggttt tcttgcgtgg
      241 gtccgtttcg cgacgcgccg ccggcgacag gggccgggcc acgcgaaggg ctggtattat
      301 acgcagcgat cacgactacg gggcagcgcg ccatgcagac ctacctgctg atcagtttca
      361 tcgccgacga caggcccggc ctggtgcaga gcctctccga ggcggttgcc gcgcacggtg
      421 gcaactggct cgagagccgc atggccaacc tggccggcaa gttcgcgggt atcgtcaacg
      481 tctcggtgcc gcaggcccgg cgccaggatc tcgagggcgc gctcggtgcc ctggaggcac
      541 agggcttgac gctgcgcctg gaggcggcgc gggccgcggc gcccgcgccg gcgcacacgc
      601 cgtggcggct cgatatcgtg ggccaggacc ggcccggcat cgtgcacgag ttctccgcgg
      661 cgctggcgag ccggcgcatc aacgtggtcg aactgcgcag cgacatcacc agtgcgccga
      721 tgagcgccga cccgctgttc gtcgcgagcg tgggcatcca cgtgccggcg gacatcgatc
      781 tggacgagct gcgcgaacgc ctcgaagcga tcgccgacga gctcacgatc gactacgcgc
      841 tggtcgcggc cgactgagga cattcccccg ccgtggacct accggcggcg ccagagcgcg
      901 cgcgcgccga ccagcagcgc gaccgcgcag gcggcggtga ggatcatcgc gacgatcagc
      961 agcaggcact gccacaggaa gccgaggatc gcggcgaggc ggaaatcgaa ggccatcgcc
     1021 gccgagacca gcagcgcgag cgtggcgacg atgccgaaca ccagcatgcg gttcgacctg
     1081 aaaagcgcga acagattcat gcatcgattc cccggtcggt gggcacggca cgcaggtgca
     1141 ggcgcacgcc atcgtggtgt ccggtgtaga cgtcgaaacg gtgcgtgatg cgctcgcgcg
     1201 cgaaatgcgc cagcagctcg ggcgcccacg gcgcctgcgc cacgccctgc gccgccgcct
     1261 ccaggcaacc gcccggtgcg cgaaatacca ggaacaggtg ctcggagccc gcatcggtga
     1321 gcgtcgcgta gagcaccagg tcctcctcgc gcggcgaggc accggtgagc gcgggctcga
     1381 gcgcgcgcag cacggcggac tggatcttct cgcccctgcc cgcctgcgcc agcagcagcg
     1441 ccggcgtgtg accgtccggc tggcacacga tgcagccgac gtgcacttcg gggttgcggt
     1501 agtacacgcg cgcgcaggcg ctgcacgcgg ggcgctcgcg atcatcgctc gccggcaccc
     1561 cggtcgtcag cggcgcgccg cacagcgcac agaaattcat cgtcatcgcg catcttcctg
     1621 catcattgcc cgattgcccg cgccgtcgcg ggcgcctcca ccagcgggcc gcgcaccgcg
     1681 gcgcgcacga tccaccagcc cgccagcgcc gccagcgcgg ctaccacgaa gccgagccgc
     1741 gcgctcatgt cccacagcag cccgctgagc agcgcgccca gcgcgccgcc ggcgccgaaa
     1801 ctgaccgccg cgtacaccgc ctggccctga ccctgctgcg cggcgccgaa atatcgccgc
     1861 aggaactcga tccccgcggc atggaagctg ccgaagctgc ccgcatgcag gcattgcgcc
     1921 accagcagca gcgccaggct gtcgacaccg aagccaatca tcagccagcg caccgcggca
     1981 agcaacaggc tcgccagcag gatcgcgcgc aggccgaagc gcgccagcag ccggtgcgcg
     2041 cacgcgaaca cgccgatctc ggcgatcacg ccgagcgacc agatcaaccc ggtcgcggcg
     2101 cgggagtaac cgtgctgctc gaggtagagg ctgaagaagc cgtagtacgg cgcatgcgag
     2161 aactgcagca ggaacgaggc cgcgaggaag gcctgcacgg ggcgcgcgcc gagcagctcg
     2221 cgcagccccc cggcgggcgg ctcgcgccgc gccaccgcgg gctcgcggat cagcagcgtg
     2281 ccggcggcga tcagcaccag cagcgcgagc agcagctgcg gcagccaggt gacgctgagg
     2341 cggtcgaaca gctggccggc gccgaccacc gcgacgatga agctcaccga gccccacagc
     2401 cgcacctgcc cgtagcggtg cacctgctcg ccgagcgagg ccagcgtgat cacctcgtac
     2461 tgcgcgagga tcgcgttcca gaacaccgag tgcagcgcca ccacggcgac cagccaccac
     2521 agatccgtgc gcaccagcag cccggcgaaa gtgatcagcg cggcgagggc gccggtgcgg
     2581 atgatcagca ggcgtcgccc gctgcggtcg gcaagccagc cccagacgtt cggcgcgacg
     2641 atgcggatgg cggccatgac cgcgagcacc acgccgatcg ccgcggcatc cagccccacg
     2701 gcctgcaggt acaggctcca gtagggcatc atcgccccga ggaaggcgta gtacaggaaa
     2761 tagaacgagg acaggcgcca gtacggaata ccggcgacgg gagcgcccat catcccgtcg
     2821 gcgctaccac gctgcccggc accaccggcg tctcgcagcg cacgtccgcg ttctgcgcgc
     2881 gatggcgcag ccagtggtcc atcaatacca gcgccagcat cgcctcggcg atcggggtgg
     2941 cacggatgcc gacgcaggga tcgtggcgcc cgtgcgtcac cacctcgatc ggctggccga
     3001 agcggtcgat cgagcgcccc ggcaggcgca tgctggaggt gggcttcagc gcgatcgaga
     3061 ccacgatgtc ctgcccgctc gagatgcccc cgaggatgcc gccggagtgg ttcgagagga
     3121 aacccgcggg cgtgatctcg tcgcggtgct cggtgccctt ctgcgccacg gccgcgaagc
     3181 cggcgccgat ctccacgccc ttcaccgcgt tgatgcccat catcgcgtgc gcgatgtcgg
     3241 cgtcgaggcg gtcgaatacc ggctcgccga ggccc
//
LOCUS       DAORUQ010000277         3255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_40495_length_3255_cov_4.447500, whole genome shotgun sequence.
ACCESSION   DAORUQ010000277 DAORUQ010000000
VERSION     DAORUQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40495_length_3255_cov_4.447500"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLW13_15400"
     CDS             complement(<1..243)
                     /locus_tag="PLW13_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71814.1"
                     /translation="MNGDLRVWVLALLLFCAGAVRATEAPQRVVSLNLCTDQILLALL
                     PRERIAMLSQLAADRTLSSAAARAEGIARFDGSVERI"
     gene            complement(240..1184)
                     /locus_tag="PLW13_15405"
     CDS             complement(240..1184)
                     /locus_tag="PLW13_15405"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQY71815.1"
                     /translation="MTDDATPSTAPAWHVLGTGSMACLWAANLAASGHEVSLIARDRS
                     RHAAYHGITLESAAGGEHFEPVLETRESATPIAQLLVCTKAYDTLDALAGIAHRLAPE
                     AAVVLLQNGMGFHDEALTLVGNARLFCALSTEGAWCPAPFHVRHAGRGVTLLGRYPHG
                     PRDEAEALARALPTRRLEIRPVDDIEHEMWRKLAVNCTINALTAVHGCPNGALLESGE
                     PGRDFAAVCFETAAVLDALGQHIIAAELPAAATEVARRTAANRSSMLQDVLARRRTEI
                     DYINGFLCRRAEAAGVPCPFNSTLCAKVHALEQGYPSP"
     gene            1257..1739
                     /locus_tag="PLW13_15410"
     CDS             1257..1739
                     /locus_tag="PLW13_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQY71816.1"
                     /translation="MPSFDVVSEVDLHAFTNAVDQASRVIETRFDFKGVDAGFERKER
                     VVTLHAEAEFQLEQMLDILRGALTKCKIDPRAVKPGDVQKSGKVVKQDVTMQHGLDTD
                     LCRKIVKMVKDAKLKVQAAIQGEQVRVTGKKRDDLQDVIALLRASDIDVPLQFENFRD
                     "
     gene            1732..3078
                     /locus_tag="PLW13_15415"
     CDS             1732..3078
                     /locus_tag="PLW13_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71817.1"
                     /translation="MTEDPARAAPARSWREALLVYTRPRVFAMIWLGFSAGLPFLLIG
                     PTLSGWQRDVGVERSVIGFFSWIGIIYSIKVLWAPVVDRVALPLLTRWLGKRRSWMLA
                     AQLVIAAGLLGMAQADPVADLALVAWFALLVAFGSATQDITIDAWRIEAMDPEFQGAM
                     AAAYVLGYRIALLVAGAGAFYLAEGASWEVAYVVMAVLMAGGIVTTLALREPDHAAAA
                     AARTLELRVEQTLGASSHRGPLGRALAFLSDAVVAPFVDFFARNGREALLILLLISLY
                     KLSDITMGVMANPFYLDLGFSKTDIADVTKIFGFFMTIAGAALGGVLVVRFGIARPLV
                     LGAVLVALTNLLFAGLAVLEPSLALLAVVVSADNLSGGIAAAVFIAYLSSLTNQAYTA
                     TQYALFSSLMTLPAKVIGGFSGVVVDAQGYMAFFLYAGLLGIPAILLSLRVLRQPP"
BASE COUNT          468 a         1083 c         1170 g          534 t
ORIGIN      
        1 gatgcgctcg accgagccgt cgaagcgcgc gatgccctcg gcgcgcgccg cggccgacga
       61 cagcgtgcga tcggcagcca gctggctgag catcgcgatg cgctcgcgcg gcagcagcgc
      121 gagcaggatc tggtccgtgc acaggttgag cgacaccacg cgctgcggcg cttcggtggc
      181 ccgcaccgcg ccggcgcaga acaacagcag cgccagcacc caaacccgca ggtcgccatt
      241 catggcgacg ggtatccctg ctccagcgcg tggaccttcg cgcacagcgt gctattgaag
      301 ggacagggca cgccggcggc ctcggcgcgc cggcacagga agccgttgat gtagtcgatc
      361 tcggtgcgcc ggcgggcgag cacgtcctgc agcatcgagg aacggttcgc ggcggtgcgg
      421 cgcgcgacct cggtggcggc ggccggcagt tccgcggcga tgatgtgctg gccgagcgcg
      481 tcgagcacgg ccgcggtctc gaagcacacg gccgcgaaat cacgccccgg ctcgcccgac
      541 tccagcagcg cgccgttcgg gcagccgtgc acggcggtga gcgcgttgat cgtgcagttg
      601 accgcgagct tgcgccacat ctcgtgctcg atgtcgtcga ccgggcggat ctcgaggcgc
      661 cgggtgggca gtgcgcgcgc gagcgcctcg gcttcgtcac gcggcccgtg cgggtagcgc
      721 ccgagcagcg tgacgccccg gcccgcgtgg cgcacgtgga acggcgccgg gcaccaggcg
      781 ccctcggtgc tgagcgcgca gaacagacgc gcgttgccga cgagcgtcag cgcctcgtcg
      841 tggaatccca tgccgttctg caacagcacc accgctgcct cgggcgccag ccggtgggcg
      901 atgccggcca gcgcgtccag cgtgtcgtag gccttggtgc acaccagcag ttgtgcgatc
      961 ggggtggcgg attcgcgcgt ttccagcacg ggctcgaagt gctcgccgcc ggcggctgat
     1021 tccagcgtta tgccgtgata cgctgcgtgc cgcgagcggt cgcgcgcgat caggctgacc
     1081 tcgtggccac tggccgcgag gttggcggcc cacaggcaag ccatcgagcc cgtgccgagc
     1141 acgtgccagg caggcgcagt ggagggggtt gcgtcgtctg tcatcgcggc attatccacg
     1201 atcggcgcgc ccgcgctcgc gcccgacgca ttcgatacgt acaagaggag aacccgatgc
     1261 cgtccttcga cgtggtgtcc gaagtggacc tgcacgcctt caccaacgcg gtcgatcagg
     1321 ccagccgcgt gatcgagacc cgcttcgact tcaagggcgt ggacgccggc ttcgagcgca
     1381 aggagcgcgt ggtgacgctg catgccgagg ccgagttcca gctcgagcag atgctcgaca
     1441 tccttcgcgg cgcgctgacc aagtgcaaga tcgacccgcg cgcggtgaaa ccgggcgacg
     1501 tgcagaaatc cggcaaggtg gtgaaacagg acgtcacgat gcagcacggg ctcgacaccg
     1561 atctgtgccg caagatcgtg aagatggtga aggacgccaa gctcaaggtg caggccgcga
     1621 tccagggcga gcaggtgcgc gtgaccggca agaagcgcga cgacctgcag gacgtgatcg
     1681 cgctgctgcg cgcgagcgac atcgacgtgc cgctgcaatt cgaaaatttc cgtgactgag
     1741 gatccggcgc gcgccgcccc ggcgcgcagc tggcgcgagg cactgctggt ctacacgcgc
     1801 ccgcgcgtgt tcgcgatgat ctggctcggc ttcagcgccg ggctgccgtt cctgctgatc
     1861 gggccgacgc tctcgggctg gcagcgcgat gtcggggtcg agcgcagcgt gatcggcttc
     1921 ttcagctgga tcggcatcat ctattcgatc aaggtgctgt gggcgccggt ggtggaccgc
     1981 gtggcgctgc cgctgctgac acgctggctg ggcaagcgcc gcagctggat gctggccgcg
     2041 caactcgtga ttgccgccgg gttgctgggc atggcgcagg ccgatccggt ggccgatctc
     2101 gcgctggtgg cgtggttcgc gctgctggtg gccttcggtt cggcgaccca ggacatcacg
     2161 atcgacgcct ggcgcatcga ggccatggac ccggagttcc agggcgcgat ggccgcggcc
     2221 tacgtgctcg gctatcgcat cgcgctgctg gtggcgggcg ccggcgcgtt ctacctcgcc
     2281 gagggcgcgt cgtgggaagt ggcctacgtg gtgatggccg tgctgatggc cggcggtatc
     2341 gtcaccacgc tggcgttgcg cgagcccgat catgcggccg ctgcggcggc ccggacgctg
     2401 gaattgcgcg tcgagcagac gctgggggcc tcatcacacc gcggccccct cgggcgggcg
     2461 ctggcgttcc tgtccgacgc ggtggtcgcg cccttcgtgg atttcttcgc gcgcaacggg
     2521 cgcgaggcgc tgctgatcct gctgctgatc agcctgtaca agctgagcga catcacgatg
     2581 ggggtgatgg ccaacccctt ctacctcgac ctcggattca gcaagaccga cattgccgac
     2641 gtcaccaaga tcttcggctt cttcatgacc atcgcgggcg cggcgctggg cggcgtgctg
     2701 gtggtgcgct tcggcatcgc gcggccgctg gtgctgggcg cggtgctggt ggcgctcacc
     2761 aatctgctgt tcgccgggct ggcggtgctc gagcccagcc tggcgcttct ggccgtggtg
     2821 gtgagtgccg acaacctgag cggcggcatc gccgcggcgg tgttcatcgc ctacctgtcg
     2881 agcctgacca accaggccta caccgccacg cagtacgcgc tgttcagctc gctgatgacg
     2941 ctgccggcaa aggtgatcgg cggcttttcc ggcgtggtgg tcgacgcgca gggctacatg
     3001 gccttcttcc tctacgccgg cctgctcggc atccccgcga tcctgctctc gctgcgcgtg
     3061 ctgcgccaac ccccgtagct cccgtgggtc cgcctacctg tccgcattca tgcggaccgg
     3121 taggcagggt cgggaatgcc tgtatccgga tgaatccgga cccacgacgg cccgtgtccg
     3181 gatgaatccg gacctagaga cggatgccgc cgtcggtttc gatgacgcgg ccggtgatgt
     3241 agtcgttctc gaaga
//
LOCUS       DAORUQ010000278         3213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_41342_length_3213_cov_5.716909, whole genome shotgun sequence.
ACCESSION   DAORUQ010000278 DAORUQ010000000
VERSION     DAORUQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41342_length_3213_cov_5.716909"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLW13_15420"
     CDS             <1..299
                     /locus_tag="PLW13_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71818.1"
                     /translation="IMAMNPFDGQTLAVAFPGISLGGMPAAGGAEAAGLGADDRRRMQ
                     EAQSGGAWSAGDTLAYDEIIDPRELRNVLLAGLRTALSRRVAPAGPAMRSGIWP"
     gene            complement(316..774)
                     /locus_tag="PLW13_15425"
     CDS             complement(316..774)
                     /locus_tag="PLW13_15425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY71819.1"
                     /translation="MIRWEWLHFAGLDTSTLYAIVAAREEVFVVEQRCAYRDADGRDP
                     AGWHLIGRGREGEVAAYLRVLLPGARFAEHSIGRVLTTAPFRDQGLGRELMLRGLSRV
                     AEQFGSVPIRIAAQAHLAHFYGSFGFVAVGDPYDEDGITHIDMLRVAPAG"
     gene            complement(791..1966)
                     /locus_tag="PLW13_15430"
     CDS             complement(791..1966)
                     /locus_tag="PLW13_15430"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQY71820.1"
                     /translation="MKRIGAVLSLLLLATVISVLAFAPGRIERNLNRIDTVAAGGTAD
                     ARAVALHASLFVADLHADSLMWARDFLQRGNRGHVDLPRLVEGNVALQVLTTVTRSPH
                     GLNYESNAADARDDITLLALVQRWPPRTWDSLTERALYQAERLEGFVARSQGGLRVIR
                     TRGDLESLIAQRGSGATTVGAVLGTEGSHALDGKLENIGRLHEAGFRIMGLQHFFDNA
                     LGGSLHGTSRAGLSEFGRAALAEMLRRGIVIDVAHSSPAVVEDVLAATDAPLLVSHTG
                     TYGHCPGPRNIPDALMQRIAARGGLIGIGFWAEAICDASPAGIAAALDAAVALLGEDA
                     VALGSDFDGAVTTTIDAAGMSAITAALLARGVDERRIVKLMGGNVRDFLLRSLPPAP"
     gene            2076..2483
                     /locus_tag="PLW13_15435"
     CDS             2076..2483
                     /locus_tag="PLW13_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQY71821.1"
                     /translation="MSNTQCALLGLASWHVIVLFLLAGFRLYTSITQKKPANTFAPDG
                     SDVPGFGQRLVRVHANCYENIPLFLALLLFALVSSQTAVTDPSAMILLGARVGQSVVH
                     MISTSVPAVLLRFALFLVQVLTVVCWLYQFLTA"
     gene            complement(2540..>3213)
                     /locus_tag="PLW13_15440"
     CDS             complement(2540..>3213)
                     /locus_tag="PLW13_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HQY71822.1"
                     /translation="KVKGLDYLAVAQEGGGENAELRDWTGQTSAPVAVWQDEPPRTDS
                     LDILFLAERLAPTPRLIPEDVEQRARMFGITREIIGRRGLGWMRRIMIFAPIMGSGQA
                     PESIRRMAERYSYRDDAAQAAPGEVARILEFLGRTLERQQQAGSRYLVGDSLSAADLY
                     WAAFSNIVSPMPPGQCPMPDFIRTAYAAIGPVVESALNPLLLAHRDFVWAEHIGLPMD
                     FLPTP"
BASE COUNT          507 a         1126 c         1076 g          504 t
ORIGIN      
        1 cgatcatggc gatgaatccc ttcgacggtc agacgcttgc cgtcgccttc cccggcatat
       61 ccctgggcgg catgccggcg gccggcggtg ccgaggccgc gggactgggc gccgatgatc
      121 gcaggcgcat gcaggaagcg cagtccggcg gtgcctggag cgccggcgac acgctggcct
      181 acgacgagat catcgacccg cgcgagttgc gcaacgtgct gctggcgggg ctgcgcacgg
      241 cgctgtcgcg ccgcgtcgcg cccgccgggc ccgctatgcg cagtggcatc tggccctgaa
      301 cctgtgggtc gggcttcagc ccgccggtgc cacgcgcagc atatcgatgt gcgtgatgcc
      361 gtcctcgtcg tagggatcac ccacggcgac gaatccgaac gagccgtaga aatgtgcgag
      421 gtgcgcctgc gcggcgatgc ggatcggcac gctgccgaat tgctccgcca cgcgcgacag
      481 tccgcgcagc atgagctcgc gccccagccc ctggtcgcgg aacggcgccg tcgtcagcac
      541 gcggccgatg gaatgctcgg caaagcgcgc accgggcagc agcacgcgca ggtacgccgc
      601 gacttcgcct tcgcggcccc ggccgatcag gtgccagccc gcggggtcgc gtccgtcggc
      661 atcgcgatag gcgcagcgct gctcgaccac gaagacttcc tcgcgtgcgg ccacgatcgc
      721 gtaaagcgtg ctcgtgtcga gcccggcgaa gtgcaaccat tcccatcgga tcatccgcgt
      781 cgtctcccgg tcagggcgcg ggcggcaggc tgcgcagcaa aaagtcgcgc acattgccgc
      841 ccatgagctt cacgatgcgg cgctcgtcca ccccgcgcgc gagcagcgcg gcggtgatcg
      901 cggacatgcc ggcggcatcg atggtggtgg tcacggcacc gtcgaaatcc gaaccgagcg
      961 caaccgcgtc ctcgccgagc agggccaccg cggcatcgag cgccgcggcg atccccgccg
     1021 gactcgcgtc gcagatcgcc tcggcccaga agccgatccc gatgagcccg ccgcgcgccg
     1081 cgatgcgttg catcagcgca tcgggaatgt tgcgcgggcc cgggcaatgg ccgtaggtgc
     1141 cggtgtggct gacaagcagc ggcgcgtcgg tcgcggccag cacgtcctcg actaccgcgg
     1201 gcgaggaatg cgcgacgtcg atcacgatcc cgcggcgcag catctcggcg agcgccgctc
     1261 gcccgaactc gctgagtccc gcgcgggagg tgccatgcag cgaaccgccc agcgcgttgt
     1321 cgaagaagtg ctgcaggccc atgatgcgaa agcccgcctc gtgaaggcgg ccgatgtttt
     1381 ccagcttccc gtcgagcgca tgggatccct cggtgccgag caccgcgccg acggtagtcg
     1441 cccccgagcc gcgttgcgca atcagcgatt ccaggtcgcc gcgggtccgg atcacgcgca
     1501 gcccaccctg cgatcgggca acgaagccct cgagccgctc ggcctggtac agcgcacgct
     1561 cggtgagcga gtcccacgtg cgcggtggcc agcgctgcac cagcgccagc agcgtgatgt
     1621 cgtcacgcgc atcggctgca ttgctctcgt agttcaggcc gtgcggcgag cgggtcaccg
     1681 tggtcaacac ctgcagcgcc acgttgccct cgacgagtcg cggcaggtcc acatggccgc
     1741 gattgccgcg ttgcaggaaa tcgcgcgccc acatcaggct gtcggcgtgc aggtcggcca
     1801 cgaaaagcga tgcatgcagc gccactgcac gcgcatcggc ggtgccgcca gccgccacgg
     1861 tatcgatgcg gttcaggttg cgctcgatgc ggccgggcgc aaacgccagc accgagatca
     1921 cggttgcgag caacagcagc gacaggaccg ccccgattct cttcatgccg tatcgacgct
     1981 ccccgtgttc atcggtcgtc tcccggatgc accgccggcg cgcgatctgc taggctcggt
     2041 gtgccgttca acaataatct tccggaggca accccgtgtc caacacccaa tgcgccctgc
     2101 tcggactcgc cagctggcac gtgatcgtgc tgttcctgct ggcgggcttc agactctaca
     2161 cgagcatcac gcagaagaaa cccgccaaca cctttgcccc tgacggcagc gatgtgccgg
     2221 gcttcggcca gcgcctggtg cgcgtccatg caaactgcta cgagaatatt ccgctgttcc
     2281 tggcgctgct cctgttcgca ctggtgagca gccagaccgc ggtgaccgat ccctcggcaa
     2341 tgatcctcct cggcgcgcgc gtcggccaga gcgtggtgca catgatctcc accagcgtac
     2401 cggccgtgct gctgcgcttc gcgctgttcc tggtgcaggt gctgacggtg gtgtgctggc
     2461 tgtaccagtt cctgactgcc tgagttcgtc cgtgccctgg gtccgcattc atgcggacat
     2521 tgtccggatg aatccggacc tacggtgtgg gcaggaaatc catcggcaag ccgatgtgct
     2581 cggcccacac gaaatcgcgg tgcgcgagca gcagcggatt cagggccgac tcgaccaccg
     2641 gcccgatagc ggcgtaagcc gtacggatga agtcaggcat cgggcactgc ccgggcggca
     2701 tcgggctgac gatgttcgag aacgcggccc agtacaggtc ggcggcgctg agcgagtcgc
     2761 caacgagata acggctgccg gcttgctgct ggcgctcgag ggtgcggccg aggaattcca
     2821 ggatgcgcgc cacctcgccc ggcgcggctt gcgcagcgtc gtcgcggtac gagtagcgtt
     2881 cggccatgcg ccgtatcgat tcgggcgcct gccccgagcc catgatcggc gcgaagatca
     2941 tgatgcgcct catccagccg agcccgcgcc gtccgatgat ctcccgcgtg atgccaaaca
     3001 tgcgcgcgcg ctgctccacg tcctcgggaa tgagtcgtgg cgtgggcgcc aggcgctcgg
     3061 caaggaacag gatgtcgagc gaatcggtgc gcggtggttc gtcctgccac accgcgacgg
     3121 gcgccgaggt ctgtcccgtc cagtcacgca actcggcgtt ttccccaccg ccctcctgcg
     3181 ccacggccag gtaatccagc cccttgacct tga
//
LOCUS       DAORUQ010000279         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_42745_length_3145_cov_6.609385, whole genome shotgun sequence.
ACCESSION   DAORUQ010000279 DAORUQ010000000
VERSION     DAORUQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42745_length_3145_cov_6.609385"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLW13_15445"
     CDS             <1..1588
                     /locus_tag="PLW13_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HQY71823.1"
                     /translation="LFDALEERSVSGVLVQVGMFALIFALTLAVTAAHLMVKRWLQLD
                     WRRWLTDRVIGHWMARGRHYRLLFTAGEHDNPDGRIAEDIRIVTETTISLAHTLVYSL
                     LVLGMFIDILWQVSGSVHLPGTGIHVSGYLVWLAFLYAGIGTVLGWSFGRPLVRSTNA
                     LQTAEADFRFGLARAREHSEAIALMHGEPMERSSATRRFVRIALQWYRQSRAYMGIVA
                     FSTGYGALLPVFPILVAAPQYIAGAMTLGVLMQAAQAFQRLTSALSWPVDNLGDMART
                     RASADRVLSLYEDMQRLDAEARAPDGNRIALAHDAHARIRIKDLCIADPEGRILLEHL
                     DLEIRRGERVLVAGDPAVTSSLFKVIGGLWPWGRGHVQLPDKDCIQFMPQRPFLPEGT
                     LRQALCYPQRPDAFANEAVLRALECSGSGWLAPRLDERDNWEQSLPLRAQQRLGFARV
                     LLLRPAWILLEEATDAFDPRGERIILEMLQHELPSSAMLTISFHSGLEALHHRKIVLN
                     RLTEERFLFEGKRENGSSPH"
     gene            complement(1582..2694)
                     /locus_tag="PLW13_15450"
     CDS             complement(1582..2694)
                     /locus_tag="PLW13_15450"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HQY71824.1"
                     /translation="MEHTSTNMRLRGVNLGGWLVLEKWMKPSLFAGLAATDETTWCAE
                     LGAAAPRLLRAHWDSFITREDFAWLAARGINAVRIPLGHWIFGPPYPYHAAYGANPHP
                     FVEGGIEVLDRAMDWAEELGLRVVLDLHAAPGCQNGFDNGGIKDVCEWHTREEYLAHS
                     VEVLGRLAARYRGRRGLHAIEVLNEPHSDVPTELLKDYYRRANRAIRAHCAPQDVAVV
                     FHDGFRPHREFLGLFQEPGFANVLFDVHRYQCFERTDIDLDIHGHLHKAGMLWKDEAM
                     EIQRELGVPAIAGEWSLGLDLKVVSLWAPGPYNHALEHMDAFRQHTALRGYAAAQLLA
                     FEHYRGWFFWSYRTETTPAWSFRDAVERGWLPAGFQ"
     gene            2769..>3145
                     /locus_tag="PLW13_15455"
     CDS             2769..>3145
                     /locus_tag="PLW13_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71825.1"
                     /translation="MQQLTTTPPGRRGLVATLAACAAWLALLYAFRFGFMEIEAEADP
                     CLNDPLGALCRARAVIGMSIHLQLFGTAALVLALAAHLRLGRLKRALALAGLFAALLA
                     LVLYNVRYGAPAAVIALLALADLG"
BASE COUNT          448 a         1094 c         1102 g          501 t
ORIGIN      
        1 gctgttcgat gcgctggagg aacgctcggt gtcgggcgtg ctggtgcagg tgggcatgtt
       61 cgcgctgatc ttcgcgctca cgctcgcggt gacggccgcg cacctgatgg tcaagcgctg
      121 gctgcagctc gactggcggc gctggctgac cgatcgcgtg atcggacact ggatggcgag
      181 agggcgccat taccggctgc tgttcacggc gggcgagcac gacaacccgg acgggcgcat
      241 cgcggaggac atccgcatcg tcaccgagac cacgatctcg ctggcgcaca cgctggtgta
      301 ctcgctgctg gtgctcggca tgttcatcga catcctgtgg caggtctcgg gctcggtgca
      361 cctgcccggc accgggatcc acgtctcggg ctacctggta tggctcgcgt tcctgtacgc
      421 cggcatcggc accgtgctcg gctggagttt cggccgcccg ctggtgcgct cgaccaacgc
      481 gctgcagacc gcggaagcgg acttccgctt cggcctggcg cgcgcgcgcg agcactcgga
      541 ggccatcgcg ctgatgcacg gcgaaccgat ggagcgctcg agcgccacgc ggcgcttcgt
      601 gcgcatcgcg ctgcagtggt accgccagtc gcgcgcctac atgggcatcg tggccttctc
      661 gaccggctac ggcgcgctgc tgcccgtgtt cccgatcctg gtcgccgctc cgcagtacat
      721 cgccggcgcg atgacgctcg gcgtactgat gcaggccgcg caggctttcc agcggctgac
      781 ctcggcgctg tcgtggccgg tggacaacct cggcgacatg gcgcgcacgc gcgcctcggc
      841 cgaccgcgtg ctgtcgctgt acgaggacat gcagcgcctc gacgccgagg cgcgcgcgcc
      901 ggacggcaac cgcatcgcgc tcgcacacga tgcacatgca cgcatcagga tcaaggacct
      961 ctgcatcgcc gatcccgagg gccgcatcct gctcgagcat ctcgacctcg agatccgccg
     1021 cggtgagcgc gtgctggtgg cgggcgaccc ggcggtgacc agcagcctgt tcaaggtgat
     1081 cgggggcctg tggccatggg gccgcggcca cgtgcagttg cccgacaagg actgcatcca
     1141 gttcatgccg cagcgcccgt tcctgcccga ggggacactg cgccaggcgc tgtgctaccc
     1201 gcagcgcccg gatgccttcg ccaacgaggc ggtgctgcgc gcgctcgagt gctccggcag
     1261 cggatggctg gcgccgcggc tggacgagcg cgacaactgg gagcagtcac tgccgctgcg
     1321 cgcgcagcag cgtctgggct tcgcgcgcgt gctgctgctg cgcccggcgt ggatcctgct
     1381 cgaggaagcg accgatgcct tcgacccgcg cggcgagcgc atcatcctgg aaatgctgca
     1441 gcacgagctg cccagcagcg ccatgttgac gatcagcttc cacagcggcc tggaagcgct
     1501 gcatcaccgc aagatcgtgc tgaaccgcct caccgaggag cgcttcctgt tcgagggcaa
     1561 gcgcgagaac ggctcatcgc ctcactgaaa gcccgccggc agccagccgc gctccaccgc
     1621 gtcgcgaaag ctccacgccg gcgtggtctc ggtgcgatag ctccagaaga accagccgcg
     1681 gtagtgctcg aaggcgagca gttgcgccgc cgcgtagcca cgcagggcgg tgtgctgccg
     1741 gaaggcgtcc atgtgctcga gcgcgtggtt gtagggcccc ggcgcccaca gcgataccac
     1801 cttcaggtcg agcccgaggc tccattcccc ggcaatcgcg ggcacgccga gctcgcgctg
     1861 gatctccatc gcctcgtcct tccacagcat gccggccttg tgcaggtggc cgtggatgtc
     1921 gaggtcgatg tcggtgcgct cgaagcactg gtagcggtgc acgtcgaaca gcacgttggc
     1981 gaagcccggt tcctggaaca gcccgaggaa ctcgcggtgc gggcgaaagc cgtcgtggaa
     2041 caccacggcg acgtcctgcg gcgcgcagtg cgcgcgtatc gcgcggttgg cgcgccggta
     2101 gtagtccttc agcagctccg tgggcacgtc ggagtgcggc tcgttcagta cctcgatggc
     2161 gtgcaggccg cggcggccgc gatagcgcgc ggcgagccgc cccagcacct cgaccgagtg
     2221 cgccaggtat tcctcgcgcg tgtgccactc gcagacgtcc ttgatgccgc cgttgtcgaa
     2281 gccgttctgg cagccagggg ccgcgtgcag gtcgagcacc acgcgcagcc cgagctcctc
     2341 ggcccagtcc atggcgcggt cgagcacctc gatgccgccc tcgacgaagg gatgcgggtt
     2401 cgcgccgtag gcagcgtgat agggataagg cggcccgaag atccagtgcc cgagcgggat
     2461 gcgcacggcg ttgatgccgc gcgcggcgag ccaggcaaaa tcctcgcgcg tgatgaagct
     2521 gtcccagtgc gcgcgcagca gtcgcggcgc ggcggcgccg agctccgcgc accacgtggt
     2581 ttcgtcggtg gccgccagcc cggcgaacag gctcggcttc atccacttct ccagcaccag
     2641 ccagccgccg aggttgacgc cgcgcaggcg catgttcgta ctcgtgtgtt ccatgggatc
     2701 cgctccgtgg gctgcggcgg gagtttatac ttcgccgggt gacgatggcg acgcgaggca
     2761 gctcaaggat gcaacaactc acgacgacgc cgcccggacg acgcggcctc gtggcgacac
     2821 tcgcggcctg tgccgcgtgg ctcgcgctgc tgtacgcctt ccgcttcggg ttcatggaga
     2881 tcgaggccga ggccgacccc tgcctcaacg acccgctcgg cgcgctgtgc cgcgcgcgcg
     2941 cagtgatcgg catgtcgatc cacctgcaac tgttcggcac cgcggcgctg gtgctggcac
     3001 tggccgcgca cctgcgcctc ggccgtctca agcgcgccct cgcgctggca ggactgttcg
     3061 cggcgctgct cgcactggtg ctctacaacg tgcgctacgg cgcgccggcg gcggtgatcg
     3121 cgctgctggc gctggcggat ctggg
//
LOCUS       DAORUQ010000280         3094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_43918_length_3094_cov_5.617966, whole genome shotgun sequence.
ACCESSION   DAORUQ010000280 DAORUQ010000000
VERSION     DAORUQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43918_length_3094_cov_5.617966"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLW13_15460"
     CDS             <1..192
                     /locus_tag="PLW13_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQY71826.1"
                     /translation="CSRCGSALVSMHCGDVLEVALGSTDGDPGVRPGEHIFVGSRACW
                     YEIADALPRFEEWPPGFEG"
     gene            complement(203..2419)
                     /locus_tag="PLW13_15465"
     CDS             complement(203..2419)
                     /locus_tag="PLW13_15465"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY71827.1"
                     /translation="MKRFAIALIVALVAAGALLYAGAQGWLGRAPAAGQPTPPVPHTA
                     SEAGTPIDAGPAAGRQILFGDLHVHTIYSTDANLMSLPMMQGDGPHPPADACDFARYC
                     SRLDFWSINDHAEGLTPPRWESTREAIRQCNALADPANPDLVSFLGYEWTQMSQLDAA
                     NHFGHKNVIFRDTAEGRVPLRPIAARNPGVSVAAFVPPLRQRLGMPLLDPRHLRSYLD
                     FNRFGREMLDVPDCPDGVDTRELPADCREWAATPVELFRKLDESGLESIVIPHGNTWG
                     LYSAMNSTWDKQLAGAMHDPKRQFLVEVYSGHGNSEEYRPWRPAIATADGLRCPEPSA
                     DYLPVCWRAGEIVRERCLAEGTDAAACDTRAAATRQRAVDAGTGMEFGVVAGAGSADW
                     LDAGQCRDCFLPAQNYRPGGATQYALAIGNFDEPGAPRRFRFGLMASSDVHSARPGTG
                     YKEYARIPMTEARGPVSERALQAMQDYDGPKLARARTPEELAQERRAVLFQMMERQSS
                     FFLTGGLVAVHADSRRREDIWAALARKEVYGTSGPRILLWFDLIDRAGGEARLPMGGE
                     ARIAQNPRFRVRAAGAFEQKPGCPQDSIDALGAGRLEKLCLGECYYPGDTRHAITRIE
                     VVRIRPQQTPGEDVSGLVEDPWRVLACEGGGEECTVEFEDEEFSAGGRETLYYVRAIQ
                     APSPTVNADPLRCEKDADGVCLRADPCVGDYRTQGEDCLAPAQERAWSSPIFLDPV"
     gene            complement(2539..2955)
                     /locus_tag="PLW13_15470"
     CDS             complement(2539..2955)
                     /locus_tag="PLW13_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71828.1"
                     /translation="MSAIGKRQSIVQLRRMLRRLDRSHSGDESGCPTCSLQRLAAQLV
                     DELEAEAQQFASPDLTPGAASVTVAVEALMSVIYALDVRTADEQGRVVQALLDHIGDV
                     VLDQLAQEEMDAAGTPGADDEGGQEPPPPRRSGSLH"
BASE COUNT          398 a         1070 c         1141 g          485 t
ORIGIN      
        1 tgctcgcggt gcggctccgc gctcgtttcc atgcattgcg gcgacgtgct ggaggtggcg
       61 ctgggtagca ccgacggtga tccgggcgtg cggccgggcg agcacatatt cgtgggatcc
      121 agggcctgct ggtacgagat cgccgatgca ctcccccggt tcgaggaatg gccgccgggg
      181 ttcgagggct gagcgttctt cctcacaccg gatccaggaa tatcggtgac gaccacgccc
      241 gctcctgtgc cggcgcgaga cagtcctcgc cctgcgtgcg gtagtccccg acacagggat
      301 cggccctgag acagacgccg tcggcatcct tctcgcagcg cagcgggtcg gcgttgaccg
      361 tgggcgaggg cgcctggatc gcgcgcacgt agtagagcgt ctcgcggccg cccgcactga
      421 attcctcgtc ctcgaactcc acggtgcatt cctcgccgcc gccctcgcag gcgagcacgc
      481 gccacgggtc ctcgaccagc ccgctcacgt cctcgcccgg tgtctgctgc ggacggatgc
      541 gcacgacctc gatgcgcgtg atcgcatggc gcgtgtcgcc cgggtaatag cattcgccga
      601 ggcagagttt ctcgaggcgt cccgcgccga gcgcatcgat cgagtcctgc gggcagcccg
      661 gcttctgctc gaaggcgccg gccgcgcgca cgcgaaaacg cgggttctgc gcgatgcgtg
      721 cctcgccgcc catcggcagc cgcgcctcgc cgccggcgcg gtcgatcagg tcgaaccaca
      781 gcaggatgcg cgggccgctg gtgccgtaga cctccttgcg cgccagcgcg gcccagatgt
      841 cctcgcgccg gcgcgaatcg gcgtgcaccg cgaccaggcc gccggtcagg aagaagctcg
      901 actggcgctc catcatctgg aacagcacgg cgcggcgctc ctgcgcgagt tcctccggcg
      961 tgcgcgcgcg cgcgagcttc gggccgtcgt agtcctgcat cgcctgcaat gcgcgctcgc
     1021 tcaccgggcc gcgcgcctcg gtcatcggga tgcgcgcgta ttccttgtag cccgtgccgg
     1081 ggcgcgcgga gtgcacgtcg gaggaggcca tcaggccgaa acggaagcgg cgcggcgcgc
     1141 ccggctcgtc gaagttgccg atcgccagcg cgtactgcgt ggcgccgccc gggcgatagt
     1201 tctgcgccgg caggaaacag tcgcggcact gtccggcatc cagccagtcg gcgctcccgg
     1261 cgccggccac cacgccgaat tccatgccgg tgccggcgtc cacggcgcgc tggcgcgtgg
     1321 cggcggcgcg cgtgtcgcat gccgcggcat ccgtgccctc ggcgaggcag cgctcgcgca
     1381 cgatctcgcc ggcgcgccag cacaccggca ggtagtcggc cgaaggctcc gggcagcgca
     1441 ggccatcggc agtggcgatg gcgggccgcc agggccggta ttcctcggag ttgccgtggc
     1501 ccgagtacac ttcgacgagg aactggcgct tggggtcatg catcgcgccg gcgagctgct
     1561 tgtcccaggt gctgttcatc gcgctgtaca ggccccaggt gttgccgtgc gggatcacga
     1621 tgctctccag gccgctctcg tcgagcttgc ggaacagttc gacgggcgtg gcggcccatt
     1681 cgcggcagtc cgccggcagt tcgcgcgtgt ccacgccgtc ggggcagtcg ggcacgtcga
     1741 gcatttccct gccgaagcgg ttgaagtcga ggtacgagcg caggtggcgc gggtcgagca
     1801 gcggcatgcc caggcgctgg cgcagtggcg gcacgaaggc ggccacgctc acaccggggt
     1861 tgcgcgcggc gatcgggcgc agcggcaccc ggccttccgc ggtgtcgcga aagatcacgt
     1921 tcttgtggcc gaagtggttc gcggcgtcca gctgcgacat ctgcgtccac tcgtagccga
     1981 ggaacgagac caggtccggg ttggcggggt cggcgagggc gttgcactgg cggatcgcct
     2041 cgcgcgtgga ctcccagcgc ggcggcgtca gcccctcggc atggtcgttg atcgaccaga
     2101 aatccagccg cgaacagtag cgcgcgaaat cacaggcgtc cgccggcggg tgcgggccgt
     2161 cgccctgcat catcggcagg ctcatcaggt tcgcgtcggt ggagtagatg gtgtgcacgt
     2221 gcaggtcgcc gaacaggatc tgcctgcccg cggccggtcc ggcgtcgatc ggcgtgcccg
     2281 cctcgctggc ggtgtgcggg accggcggcg tcggctggcc cgctgccggt gcccggccga
     2341 gccagccttg cgcgcctgca tagaggagcg cgccggcggc cactagcgcc acgatcaatg
     2401 cgatggcaaa gcgcttcatc ctgcctcccg gttcttgttg tgagcgcgcg ccgggaagcg
     2461 gccggtcgcg cgggattcgg gtcggcgcga ggcgcctgcg cgggatggta ctccgcgcgg
     2521 ggagctttgg tcagtcgttc agtgcagaga gccgctgcgc cggggcggcg ggggttcctg
     2581 cccgccctcg tcgtcggcgc cgggcgtgcc ggcggcgtcc atttcctcct gagccagctg
     2641 gtccagcacc acgtcgccga tgtggtccag cagcgcctgc accacgcgcc cctgctcgtc
     2701 ggcggtgcgc acgtcgaggg cgtagatcac ggacatcagc gcctcgacgg ccacggtcac
     2761 gctggcggca ccgggcgtca gatcggggga ggcgaactgc tgggcctcgg cctcgagctc
     2821 gtcgaccagt tgcgcggcga ggcgctgcag gctgcaggtg gggcagccgc tctcgtcgcc
     2881 gctgtgcgag cggtcgagcc ggcgcagcat gcggcgcaac tggacaatgg actggcgctt
     2941 gccgatcgcg ctcatggggc aatctcctgc tggactggtc ccgcctgctg gcgggcacgg
     3001 cattatacgt gccggcggcc ggctccgggg cggcggccat gtgggttgat gggatggtcc
     3061 cctccctcct taccggcatc gagtgccaga ctgg
//
LOCUS       DAORUQ010000281         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_44803_length_3057_cov_5.427382, whole genome shotgun sequence.
ACCESSION   DAORUQ010000281 DAORUQ010000000
VERSION     DAORUQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44803_length_3057_cov_5.427382"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PLW13_15475"
     CDS             complement(<1..729)
                     /locus_tag="PLW13_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQY71829.1"
                     /translation="MSDVVEGVTQQSDAAWPSNWRTTAHSISSGRYIDPAFAALEFEK
                     LWSRVWQAAARLDEIPQGGDYTVYEIGDQSVLLVRVDAATVKAYHNFCPHRGTALGEG
                     CGKFEHDRIICPFHGWRWDLRGQVQYVLERQEFRNGELRDSDVALKEVSVAVFAGFVF
                     INLARNPEPFDDFIAPVRQLIEDLAIGDMHHYWWKEIAAPGNWKVAQEAFFESYHVPT
                     THPQLETAGADFIFGDQSGPAVDFS"
     gene            1427..2593
                     /locus_tag="PLW13_15480"
     CDS             1427..2593
                     /locus_tag="PLW13_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HQY71830.1"
                     /translation="MTDKTLDCSDLDRHMGKPIDPGRLKEPLANNDIRRWVQAMHYPN
                     RVHYEDEFAAQSRFGRIVAPQSFAVATDDGHGTAPACVGLIPNSHLIFGGDEWWFHGP
                     RIYGGDLIHNERVPLDYVVKDTKFAGPTCFQRGDNRYYNAQGDLIATQRSTSIRYRAD
                     LAREMGSLDATEDPEWTDQQLLQLEEEKFSWIRMLHELGHGKRYWDEVNVGDKLPTRV
                     IGPHSIASFTTEWRAYLFTIWCGVIRRTDLDMEALGFTADMAGKEQDPVLEKENPEQT
                     DGAYIGPSRGHLFPRWARYVGMPRSYGYGASMGAWILDYLGGWAGEWGQVVHSNCAYR
                     GPAFTGDITIMNAEVIGKLVDDQGRSIVQVDFKMSNQLGTVMSTAKAEIELPKK"
     gene            2661..>3057
                     /locus_tag="PLW13_15485"
     CDS             2661..>3057
                     /locus_tag="PLW13_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71831.1"
                     /translation="MGSLSGKVAVVTGASRGIGKGIALALASEGATVYVTGRTVTPGS
                     SPLPGTVGETAAQCNSRGGTGVAVQVDHGRDDQVAALFERVEREQGRLDILVNNAFAL
                     PDDLTQPKPFWEKPLSNWEMIDVGVRSNFV"
BASE COUNT          556 a          990 c          980 g          531 t
ORIGIN      
        1 gctgaagtcc accgcgggcc cgctctggtc gccgaaaatg aaatccgcgc ccgccgtttc
       61 cagctgcggg tgcgtggtag gcacgtggta ggactcgaaa aaggcttcct gggccacttt
      121 ccagttgccg ggcgccgcga tctccttcca ccagtaatgg tgcatgtcgc cgatcgcgag
      181 gtcctcgatg agctggcgca ccggcgcgat gaaatcgtcg aagggctcgg gattacgggc
      241 cagattgatg aagacgaagc cggcgaacac ggcgacgctg acttccttca gcgcgacgtc
      301 gctgtcgcgc agttcgccgt tgcggaactc ctggcgctcg agcacgtact ggacctggcc
      361 gcgcaagtcc cagcgccagc cgtggaacgg gcagatgatg cgatcatgct cgaacttccc
      421 gcagccctcg cccagcgcgg tgccgcgatg cgggcagaag ttgtgatagg ccttgaccgt
      481 ggcggcgtcc acgcggacca gcagcacgga ctggtcgccg atctcgtaga ccgtgtagtc
      541 accgccttgc gggatctcgt cgaggcgcgc ggcggcttgc cagacgcggc tccacagttt
      601 ttcgaactcc agcgccgcga aagccggatc gatgtaccgc ccggagctga tgctgtgcgc
      661 cgtcgtacgc cagttcgacg gccaggctgc gtcgctttgc tgggttacgc cctcaactac
      721 gtcgctcatc tcccgtatct ccggcatcca ctgtttgctt gttgtttgtc gatcttcaga
      781 ccaggcgctg ggacgccgcg actctaccgc atcggaaaga ccccatgcca ctcggcgcga
      841 tgcaatccgg ccccgcattg ccgccgccgg gagatagccg gcgatgcatg agcatctccc
      901 cgggacaccg tcccgatatc acccatgtcg cgatcatagc gccgcacgcc atgcggctca
      961 atttatttgt ccggacacat tgagcagcag ccggtatcta tccgttgata ttacgaagtt
     1021 gatatagtgc gtcgggtgca aatatatccg gacatattca atctgttgtc cgttgtcgcg
     1081 actcctgccc ctggcggcct gccttcccag accgcgggca gacgcaacgg gggaggactg
     1141 ttgcacggtg ggcagccggc acgagaagga agctattggt atgccactgc aaaacgggcg
     1201 ctacacgagc gcgggcacat ccggcctgac cgacgggtgg acgctggcgc ggctcacggt
     1261 tacttctggc gatgccgctt tccggtgcga tgggaccgtt tgcgggcatc gccgccgccc
     1321 aggacggcat tctctaccgt tgcgctgacg cggacgttag catcctggca ctgaaaccgg
     1381 cgcaagacgc cacctgacaa caactttcgc ttgactggag tcgcacatga ccgacaagac
     1441 gctggactgt tccgatctcg accgccacat gggcaagccg atcgatccgg gtcgcctgaa
     1501 ggaaccgctg gcgaacaacg acatccggcg ctgggtgcag gcgatgcact acccgaaccg
     1561 cgtgcactac gaggacgagt tcgccgcgca gagccgcttc gggcgcatcg tggcgccgca
     1621 atccttcgcc gttgccaccg atgacggcca cggcaccgca ccggcctgtg tgggcctcat
     1681 ccccaattcg cacctgatct tcggtggcga cgagtggtgg ttccacggcc cgcgcatcta
     1741 cggcggcgac ctgatccaca acgagcgcgt gccgctcgac tacgtggtca aggacaccaa
     1801 gttcgcgggg ccgacctgct tccagcgtgg cgacaaccgc tactacaacg cgcagggcga
     1861 cctgatcgcc acgcaacgtt ccaccagcat ccgctatcgc gcggacctcg cgcgcgaaat
     1921 gggctcgctg gatgccaccg aggatcccga gtggacggat cagcaattgc tgcagctgga
     1981 ggaggagaag ttctcctgga tcaggatgct gcacgagctg gggcacggca agcgctattg
     2041 ggacgaggtg aacgtcggcg acaagctgcc gacccgcgtc atcggcccgc acagcatcgc
     2101 gagcttcacc accgagtggc gcgcctacct gttcacgatc tggtgcggcg tgatccgccg
     2161 caccgatctg gacatggaag cgctcggctt caccgcggac atggccggca aggaacagga
     2221 tccggtgctc gagaaggaga atcccgagca gacggacggc gcctatatcg gtccgtcgcg
     2281 cggtcatctc ttcccgcgct gggcccgtta tgtgggcatg ccgcgctcct acggttacgg
     2341 cgcgtcgatg ggcgcgtgga ttctcgatta tctcggcggc tgggccggcg agtggggtca
     2401 ggtcgttcat tccaactgcg cctatcgcgg ccccgcattc accggcgata tcacgatcat
     2461 gaacgccgag gtgatcggca aactcgtcga cgatcagggc cgttcgatcg tgcaggtcga
     2521 cttcaagatg agcaaccagc tcggcacggt catgtcgacg gccaaggcgg aaatcgaact
     2581 gccgaaaaag taacgggcca ccgcgcgggg ccggcgcgca agcgccggcc ccgcgacaac
     2641 cgcaaccacg aggagacggc atgggatcac tttccggcaa ggtcgccgtg gtcaccggcg
     2701 ccagtcgcgg tatcggcaag ggaatcgcgc tggcccttgc gagcgaaggg gccacggttt
     2761 atgtcacggg gcgtacggtt acgccgggct cctcgccgtt accgggcacc gtcggcgaga
     2821 ccgccgcgca atgcaacagt cgcggcggca cgggcgtagc cgtccaggtc gatcatggca
     2881 gggacgacca ggtcgcagcc ctcttcgagc gggtcgagcg cgagcagggc cgcctcgaca
     2941 tcctggtcaa caatgcgttc gcgttgccgg acgacctgac acagccgaag ccgttctggg
     3001 agaagccgct ctccaattgg gagatgatcg atgtcggcgt gcgctcgaat ttcgttg
//
LOCUS       DAORUQ010000282         3037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_45270_length_3037_cov_5.825620, whole genome shotgun sequence.
ACCESSION   DAORUQ010000282 DAORUQ010000000
VERSION     DAORUQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45270_length_3037_cov_5.825620"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..580)
                     /locus_tag="PLW13_15490"
     CDS             complement(128..580)
                     /locus_tag="PLW13_15490"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HQY71832.1"
                     /translation="MSLLMKILMSPSGLAFDRALVRWSGESLLNRVFARQAGFAPQPA
                     LLLETRGRRSGTPRQAVLPWFRIDGKLMVVGSKGGMPTDPAWVSNLRARPDVSVYIAR
                     KRHAARARIATGEERAALWTALTARVPTYAHYQSLTTREIPVVILEIA"
     gene            726..1541
                     /gene="murI"
                     /locus_tag="PLW13_15495"
     CDS             726..1541
                     /gene="murI"
                     /locus_tag="PLW13_15495"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQY71833.1"
                     /translation="MSDHRPVGVFDSGVGGLSIVREMRRQMPRLDIVYVSDARYLPYG
                     EKSDAFIRERAFAISDFLLAAGARALVIACNTATAAAAHALRAHYALPIVGIEPAVKP
                     ASQATRSGIVGILATAGTLQSRRYAGLLERFGHFTRVLSQPASGLVEQVEAGDLDGPR
                     TLALLRSCLQPLLDAGADTVVLGCTHYPFLLEGIRAVVGPGVQVIDPGAAVARRVAQV
                     LGAAVDGGGLLRCYTSGDAPAQQALMSRLLGEAVMVQPLPEASCVPPRVALAR"
     gene            1561..2085
                     /locus_tag="PLW13_15500"
     CDS             1561..2085
                     /locus_tag="PLW13_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY71834.1"
                     /translation="MPPDPATVTTFYLEMRAPAELRPASGRDALAVRECEIPQFELNR
                     FLYRFVGAAWEWTDKLGWSDAQWRAWAEDPALRTWVAYHRGSPAGYFELHKQPGDAVE
                     IAYFGLAQAFIGRGFGGELLTRCIGCAWEWGASRVWVHTCSLDHPAALANYRARGMRL
                     YHREIHPRKGIETS"
     gene            2082..2951
                     /locus_tag="PLW13_15505"
     CDS             2082..2951
                     /locus_tag="PLW13_15505"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HQY71835.1"
                     /translation="MKSRPRRSALYMPGSNARALDKARTLPADMLILDLEDAVAPERK
                     PAARQQVREAIAAGGYGRRELLVRVNALDTPWGAEDLRAFAGAGADGIVLSKVASPAD
                     VLQAVRILEASGAPDSLALWFMAETARCILHIDAIAGAHPRLAGILPGTSDLAHELRV
                     RHTADRLGFIAALNLCVLAARAHGLDVLDGVHLDLEDDAGLRRACEQGRDLGFDGKTL
                     IHPRQLEAANAVFAPAADEIDRAREIIAAWREASARGSALVVIGGKLVENLHVAEAER
                     LLAVAEAVAQMGW"
BASE COUNT          408 a         1094 c         1099 g          436 t
ORIGIN      
        1 cgtactcgtg cgtgccccgc aggacaatgg cgggcagcac agccagcgac gcaccgggcc
       61 gctcgatcgc aacgaaaggg cgtttgcgac accctcttca gcccgacaag ggccctctag
      121 cccggggcta cgcgatctcg aggatcacca ccggtatctc gcgcgtcgtg agcgactggt
      181 agtgggcgta ggtgggcacg cgcgccgtga gcgcggtcca cagcgccgcg cgctcctcgc
      241 cggtggcgat gcgcgcgcgg gcggcatggc gcttgcgggc aatgtaaacg ctaacgtccg
      301 ggcgcgcgcg caggttgctg acccaggccg ggtccgtggg catgccgccc ttgctgccca
      361 ccaccatcag tttgccgtcg atacgaaacc acggcagcac ggcctggcgc ggcgtgccgc
      421 tcctgcgccc gcgggtttcc agcagcagcg ccggttgcgg cgcgaagccg gcctggcgcg
      481 cgaacacgcg gttcagcagc gactcgccgc tccagcgcac cagcgcacgg tcgaaggcca
      541 ggcccgaggg cgacatcagg attttcatca gcagcgacat gggcgcgttc cccggggaat
      601 acacgggcag tttacccaaa tgccggcgca tcaggctgtc cgtcggttcc cggcgttgct
      661 ccgcgcgccc gcgcttcatt accatggcgg accattcacg caccgcgccg ggggttgcgc
      721 accacatgtc cgaccatcgc cccgtgggcg ttttcgattc cggcgtgggc gggctctcga
      781 tcgtgcgcga gatgcgccgg cagatgccgc gcctggacat cgtctacgtc tccgatgcgc
      841 gctaccttcc ctatggcgag aaatccgatg cgttcatccg cgagcgcgcc ttcgcgatca
      901 gcgatttcct gctcgcggcc ggtgcgcgcg cgctggtcat tgcctgcaac accgccaccg
      961 cggccgcggc gcacgcgctg cgcgcgcact acgcgctgcc catcgtcggc atcgagccgg
     1021 cggtgaagcc ggcctcgcag gccacgcgca gcggcatcgt cggcatcctc gccaccgccg
     1081 gcacgctgca gagccgccgg tacgccgggc tgctcgagcg tttcgggcat ttcacgcgcg
     1141 tgctgagcca gccggcgagc gggctggtgg agcaggtcga ggcgggcgat ctcgacgggc
     1201 cgcgcacgct ggcgctgctg cgcagctgcc tgcagccgct gctcgatgcc ggcgccgaca
     1261 ccgtggtgct cggctgcacg cattacccgt tcctgctcga gggcatccgc gccgtggtcg
     1321 gtccgggcgt gcaggtgatc gaccccggcg ccgcggtggc gcgtcgcgtc gcgcaggtgc
     1381 tgggcgcggc ggtcgatggc ggcggcctgc tgcgctgcta caccagcggc gacgcgccgg
     1441 cgcagcaggc gctgatgagc cgcctgctgg gcgaggcggt catggtgcaa ccgctgcccg
     1501 aagccagttg cgtgccgccg cgcgtcgcgc tggcgcgctg aatccgtgac gcgaaggccc
     1561 atgccgcccg atcccgccac cgtgaccacg ttttacctcg agatgcgcgc acccgccgag
     1621 ctgcggcccg cgagcggccg cgacgcgctc gcggtgcgcg agtgcgagat cccgcagttc
     1681 gagctgaacc gcttcctgta ccgtttcgtc ggcgccgcgt gggaatggac cgacaagctg
     1741 ggctggagcg acgcgcaatg gcgggcctgg gccgaggatc cggcgctgcg cacctgggtt
     1801 gcgtatcacc gcggttcgcc cgcgggctat ttcgagctgc acaagcagcc cggcgacgcc
     1861 gtcgagatcg cgtatttcgg gctcgcgcag gcgttcatcg gccgcggttt cggcggcgag
     1921 ctgctcacgc gctgcatcgg gtgcgcgtgg gagtggggcg cctcgcgcgt gtgggtgcac
     1981 acctgctcgc tcgaccaccc cgcggcgctg gcgaactacc gcgcgcgcgg catgcggctc
     2041 tatcaccggg aaatccaccc tcgcaaaggc atcgagacat catgaaatcc aggccgcgcc
     2101 gttcggcgct ctacatgccc ggttccaacg cgcgcgcgct cgacaaggcg cgcacgctgc
     2161 cggccgacat gctgatcctc gacctcgagg acgccgtggc gcccgagcgc aagcccgcgg
     2221 cgcgccagca ggtgcgcgag gcgatcgccg cgggcggcta cggcaggcgc gaactgctgg
     2281 tgcgcgtcaa cgcgctcgac acgccgtggg gcgcagagga cctgcgcgcc ttcgccggcg
     2341 cgggcgccga cggcatcgtg ctgtcgaagg tcgcctcgcc cgcggacgtg ctgcaggccg
     2401 tgcggatact cgaggcgagc ggcgcaccgg attcgctcgc gctgtggttc atggccgaga
     2461 ccgcgcgctg catcctgcac atcgacgcga tcgccggcgc gcacccgcgc ctcgccggca
     2521 tcctgcccgg tacctctgat ctcgcgcacg agctgcgcgt gcgtcacacc gcggaccggc
     2581 tcggcttcat cgccgcgctg aacctctgcg tgctggcggc gcgcgcgcac gggctcgacg
     2641 tgctcgacgg cgtgcacctg gatctcgagg acgacgccgg gctgcgccgg gcctgcgagc
     2701 aggggcgcga cctcggcttc gacggcaaga ccctgatcca tccgcgccag ctcgaggcgg
     2761 ccaacgcggt gttcgcgccg gcggcggacg agatcgatcg cgcgcgcgag atcatcgccg
     2821 cctggcgcga ggccagcgcg cgcggcagcg ccctggtggt gatcggcggc aaactggtgg
     2881 agaacctgca cgtggccgag gccgagcgcc tgctcgcggt ggccgaggcg gtggcgcaga
     2941 tggggtggta gggcgatcta ctcgtggcgc agcgcctgga tcgggtcgag ccgagcggcg
     3001 cggcgcgccg ggaagtagcc gaacaccacg ccgatca
//
LOCUS       DAORUQ010000283         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_45483_length_3028_cov_5.225698, whole genome shotgun sequence.
ACCESSION   DAORUQ010000283 DAORUQ010000000
VERSION     DAORUQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45483_length_3028_cov_5.225698"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLW13_15510"
     CDS             <1..620
                     /locus_tag="PLW13_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71836.1"
                     /translation="LERVLGVGHSMGGHAVVLAAAARPDRFRGLLLVDPVIVAPEIGA
                     LVAGGVSAESHPIRRRRNRWDSAQEMFDSFRAKEPYSRWDAGVLMDYCRYGLEPAEGG
                     GYVLACPPDLEAEVYAGAGTSDIYALLPRVRVPVEIIRARARRPEESLFDFSASPTWD
                     RLAAQFPDATDEQLPDCSHFIPMERPRWMAERIARADARMVAGTV"
     gene            624..1877
                     /locus_tag="PLW13_15515"
     CDS             624..1877
                     /locus_tag="PLW13_15515"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HQY71837.1"
                     /translation="MELSLFARRFGGDSGVVDLMRDLGEAVSIHPDMIMMGGGNPGRV
                     PAAERLYRERLRAVLDDPALAHGMLGVYQSPRGDERFRAALARMLREECGWAVGPENV
                     AVANGSQSAFFVLFNLFAGEFEGGRRKHILLPLVPEYIGYREVGLSDGMFHAERPAIE
                     RLPGQLFKYRVDFGQLAPDASAGAICVSRPTNPTGNVLGDAEIARLDALARAHGIPLI
                     IDGAYGTPFPHMLFTGATPHWNDNTVVVLSLSKLGLPGLRTGIIVAREEIVAAYARAN
                     TILSLACGNAGPALATALLEHGELLALAREHIGPFYRDKAARALEALRHEIAGLPCRI
                     HTPEGAMFLWLWCEGIPGGSHALYQRLKQRGVLVVPGRDFFPGLVGPWEHRDECLRLS
                     YAQDEERVRAGIAIIGDELRRSYGG"
     gene            1881..>3028
                     /locus_tag="PLW13_15520"
     CDS             1881..>3028
                     /locus_tag="PLW13_15520"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQY71838.1"
                     /translation="MSRAFLDHLAGELAQIECEGLTRHERQIATPQDAVIRVAGGGQV
                     INFCANNYLGLANHPALIAAAREGLERFGFGMASVRFICGTQTLHRELEEGLSAFLGT
                     EDTILYSSCFDANGGLFETILDEQDAVISDALNHASIIDGIRLSKARRYRYANNDLGD
                     LERQLRQADADGVRFKLIATDGVFSMDGIVARLDAICDIAARYDALVMVDDSHAVGVI
                     GARGRGTPEHCGVTGRVDIITGTLGKALGGASGGYTSGRREIVQLLRQRSRPYLFSNS
                     LAPPIVAASLRVLGMLESGDALRQRLQENSAWFRAAMAAAGFRLAGAGHPIVPVMIGD
                     ARMAAQMAERLLAEGIYVVAFSYPVVARDAARIRVQISAAHTREHLERA"
BASE COUNT          423 a         1038 c         1101 g          466 t
ORIGIN      
        1 ggctggagcg cgtgctcggc gtcgggcact cgatgggcgg gcatgccgtg gtgctcgctg
       61 ccgcggcacg gcctgatcga ttccgcggcc tgctgctggt cgacccggtg atcgtggcgc
      121 cggaaatcgg ggcgctggtg gcgggcggcg tgtcggccga atcgcacccg atccgccggc
      181 gccgcaaccg ttgggactcg gcgcaggaga tgttcgactc cttcagggca aaggaaccct
      241 attcgcgctg ggacgccggt gtgctcatgg actactgccg ctacgggctg gagccggcag
      301 aggggggcgg ctatgtgctc gcgtgcccgc ccgacctgga ggccgaggtc tatgctggcg
      361 cgggcaccag cgacatctat gcgctgctgc cgcgcgtgcg cgtgccggtg gaaatcatcc
      421 gcgcacgggc gcgccgcccc gaggagagtc tcttcgactt ttcggcgtca ccgacgtggg
      481 atcgcctggc cgcgcagttc ccggacgcga ccgacgagca actccccgac tgctcgcatt
      541 tcatcccgat ggagcgcccg cgctggatgg cggagcgcat cgcgcgtgcc gacgcgcgca
      601 tggtcgccgg cacggtctga gcgatggagc tctcgctgtt tgcccgacgc ttcggcggcg
      661 actcgggcgt ggtcgacctg atgcgcgacc tcggcgaggc ggtctcgatc catcccgaca
      721 tgatcatgat gggcggcggc aatcccggtc gcgtgccggc cgcggaacgg ctctaccgcg
      781 agcgcctgcg cgcggtgctg gacgatcccg cgctcgcgca cggcatgctc ggcgtgtacc
      841 agtcgccccg gggcgacgaa cgctttcgcg cagcgctggc acgcatgctg cgcgaggaat
      901 gcggctgggc ggtggggccg gagaacgtcg ccgtcgccaa cggcagccag tcggccttct
      961 tcgtgctgtt caacctgttc gccggcgagt tcgaaggcgg gcggcgcaag cacatcctgc
     1021 tgccgctggt gccggaatac atcggctatc gcgaggtcgg attgagcgac ggcatgttcc
     1081 atgccgagcg gcccgcgatc gagcgtcttc ccgggcaact gttcaagtac cgcgtcgact
     1141 tcgggcagtt ggcgcccgac gcctcggccg gcgcgatctg cgtgtcgcgc ccgaccaacc
     1201 ccacgggcaa cgtgctcggc gacgcggaga tcgcgcgcct cgatgcgctg gcgcgcgcgc
     1261 acggcatacc gctgatcatc gacggcgcct acggcacgcc gttcccgcac atgctgttca
     1321 cgggggccac gccgcactgg aacgacaaca cggtggtggt gctcagcctg tcgaagctgg
     1381 ggctgccggg gctgcgcacc gggatcatcg tggcgcgcga ggagatcgtt gcggcctatg
     1441 cgcgcgcgaa cacgatcctg agcctcgcct gcggcaacgc gggacccgcg ctggcgaccg
     1501 cgctcctcga acacggcgag ctgctggcgc tggcgcgcga gcacatcggc ccgttctacc
     1561 gcgacaaggc ggcgcgcgcg ctcgaggcac tgcgccacga gatcgccggc ttgccgtgcc
     1621 gcatccacac gcccgagggg gcgatgttcc tgtggctctg gtgcgagggc atccccggcg
     1681 gaagccacgc gctgtaccag cgcctgaagc agcgcggggt gctggtggtg cccgggcgcg
     1741 actttttccc cggcctcgtc ggcccctggg agcaccgcga cgagtgcctg cgcctgagct
     1801 atgcgcagga cgaggagcgc gttcgggccg gcatcgcgat catcggcgac gagctgcgcc
     1861 gcagctacgg aggctagggc atgtcgcgcg cattcctcga tcatctggca ggcgagctcg
     1921 cgcagatcga gtgtgagggc ctgacccggc acgagcgcca gatcgcgacg ccgcaggacg
     1981 cggtgatacg cgtggccggc ggtgggcagg tgatcaactt ctgcgccaac aactacctcg
     2041 ggctcgcgaa ccaccccgcg ctgatcgccg ccgcgcgcga gggactggag cgcttcggct
     2101 tcggcatggc ctcggtgcgc ttcatctgcg gcacgcagac gctgcaccgc gagctggagg
     2161 aggggctgag cgccttcctc ggcaccgagg acaccatcct gtattcctcg tgcttcgacg
     2221 ccaacggcgg attgttcgag accatcctgg acgaacagga cgcggtgatc tccgacgcgc
     2281 tgaaccatgc ctcgatcatc gacggcatcc gcctcagcaa ggcgcggcgt taccgctacg
     2341 ccaacaacga cctcggggat ctcgagcgcc agctgcgcca ggccgatgca gatggcgtgc
     2401 gcttcaagct gatcgccacc gatggcgtgt tctcgatgga cggcatcgtc gcgcgcctcg
     2461 acgcgatctg cgacatcgcc gcgcgctacg atgcgctggt gatggtcgac gattcgcacg
     2521 cggtcggcgt gatcggcgcg cgcggacgcg gcacgccgga gcactgcggc gtgacggggc
     2581 gcgtggacat catcaccggc acgctgggca aggcgctcgg cggcgcctcg ggcggctaca
     2641 cctcgggccg gcgcgagatc gtgcagttgc tgcgccagcg cagccggccg tacctgttct
     2701 cgaactcgct ggccccaccc atcgtcgcgg catcgctgcg cgtgctgggc atgctggagt
     2761 cgggcgatgc gctgcgccaa cgcctgcagg agaacagcgc ctggttcagg gccgcgatgg
     2821 cggcggccgg attccgcctc gcgggcgccg gacacccgat cgtgccggtg atgatcggcg
     2881 atgcgcggat ggcggcgcag atggcggagc ggctgctcgc cgagggcatc tacgtggtcg
     2941 ctttctcgta cccggtggtg gcgcgcgatg cggcgcgcat ccgcgtgcag atctcggcgg
     3001 cgcacacgcg cgagcacctc gagcgcgc
//
LOCUS       DAORUQ010000284         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_45684_length_3020_cov_6.030017, whole genome shotgun sequence.
ACCESSION   DAORUQ010000284 DAORUQ010000000
VERSION     DAORUQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45684_length_3020_cov_6.030017"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..>1151
                     /locus_tag="PLW13_15525"
     CDS             211..>1151
                     /locus_tag="PLW13_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HQY71839.1"
                     /translation="METNAFSNAAPRLDIDPQTLPIEALEDEITTLSASITVATWRLL
                     VLIAELDRRGKWGSWGLASCAHWLNWKCGIALGAAREKVRVAHALAGLPKVSDALSRG
                     ELSYSKARAITRVATPESEDYLLMIARHGTAAHIEKLVRTYRRIDRLHEAARANRHHA
                     QRALHWRYDDDGSMLITVRLPAEQGALVLKAITAAAESARAERDGADREPVTAENVSA
                     ETSAPSSNSFDNAESPAAARRADALCHMAEQFLAREAVSSRQAERFQVVIHVDEQTLA
                     DDVSAQRCHIEHGGALAPETARRLACDASVVSMGAPL"
     assembly_gap    1152..1251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1379..2758)
                     /locus_tag="PLW13_15530"
     CDS             complement(1379..2758)
                     /locus_tag="PLW13_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY71840.1"
                     /translation="MSGTFVDALGRRLAPDRRGLHSAVAIASVLAAMVLVVLDAAIAN
                     VALPTIARSLRVTSAQSVWIITAYQAALVMALLPCAALGESLGFRRVFTAGVALFVGA
                     SGLCALSPSLPLLVAARFLQGLGGAAVMALGLALLRVVVSQQRLGAAIGWNALAVALS
                     SAAGPAIGAAILSATSWPWLFAVNLPLGVAVLFATRALPEAGGTGRRLDLCSVALSAA
                     AFASLVIAAELLPARPALAAVLVGGAAVSLVTLVRREMSKEVPLIPLDLLRAASFRIS
                     VIASVLCFSGVAASLVALPFYLQHGLGLNAWMTGLYMTPWPLTVAIAAPLAGRLADRV
                     STARLCVAGAVCLAIGLAALALWPLRAHPLQLVMLTMLCGLGFGFFQVPNNRNMLLSV
                     PGERSGAAGGMQGTARLVGQTAGAIIMTQLFTLTSDAAAPRIGLGIAAVLTLAAGLVS
                     ALRVRTIAS"
     gene            complement(2755..>3020)
                     /locus_tag="PLW13_15535"
     CDS             complement(2755..>3020)
                     /locus_tag="PLW13_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71841.1"
                     /translation="QGEIEMGTYARIERPGGNVELVKRDSTLLRPGQVRVYLPGDIHD
                     TRCVKGPALLFRFTERDLKREDQEERRVTRYVERNGAWTAGAP"
BASE COUNT          567 a         1035 c          934 g          384 t
ORIGIN      
        1 ccgacaccaa cgagctctac caggaggcgt gccgcatcgc cgtcgagacc gggccggcga
       61 tccgtgcgtg aacatcctgg caaccgagcc agccacaccg caacagacac atcacgacgc
      121 gttcttgtaa cacatcgatc tagcgaaacg atggagccga atcgcgatac tgtataaaat
      181 cacatgcacg ttcgcaggga gcgatttcgc atggaaacca acgcgttctc gaatgctgcg
      241 ccacgcctcg acatcgatcc gcaaaccctt ccgatcgagg ctctcgaaga cgagatcacc
      301 acgctcagcg cgagtatcac ggtcgccacc tggcgcctgc tggtgctgat cgcggagcta
      361 gatcgccgcg gcaagtgggg atcgtggggg ctcgcgtcct gcgcgcactg gctcaactgg
      421 aaatgcggca tcgcgctcgg cgccgcgcgc gagaaagtgc gcgtggcaca cgcgctcgcc
      481 ggactgccca aggtgagtga cgcgctatcg cgcggcgagt tgagctactc caaggcgcgc
      541 gccatcacgc gtgtcgccac gcccgaaagc gaggactacc tgctcatgat cgcgcgccac
      601 ggcacggccg cgcacatcga gaagctggtg cgcacctatc gccgcatcga ccgtctgcac
      661 gaggccgcac gggccaatcg ccaccacgcg cagcgcgccc tgcactggcg ctacgacgat
      721 gacggctcga tgctgatcac ggtgcgcctg cccgcggagc aaggcgcgct ggtgctgaag
      781 gcgatcacag cggccgcgga gtccgcgcgc gccgagcgcg atggcgccga tcgtgaaccc
      841 gttacggcgg agaacgtttc cgcggaaacg tcggccccct cctccaacag tttcgacaac
      901 gccgaatcgc ccgccgccgc gcggcgcgcc gatgcgttgt gccacatggc ggaacagttc
      961 ctcgcgcgcg aagccgtgag ctcgcgccag gccgaacgct tccaggtggt aatacacgtg
     1021 gacgagcaga cgctggccga cgacgtcagc gcccaacgct gccacatcga gcacggcggc
     1081 gcactcgcgc cggagacggc gcgacgactc gcctgcgatg cgtcagtcgt gagcatgggc
     1141 gcgccgctcg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgcgcgt
     1261 tgcggcgccg acgtttccgc ggagacgtgc acctcgctct ggcagggcga gcgcatggac
     1321 ctggacatgg ccgttggcgg actgatgcgc ctgaacggcg tgacgtgatt cagcaccgct
     1381 agctcgcgat cgttctgaca cgcagcgcac tcacgaggcc cgccgccaga gtcagcacgg
     1441 ctgcgattcc cagaccgatc cgtggcgccg ccgcgtcgga tgtcagcgtg aagagctgcg
     1501 tcatgatgat cgcgcccgcc gtctggccca ccaggcgcgc ggtgccctgc attccgcccg
     1561 ccgcgccact gcgctcaccc ggcaccgaca gcagcatgtt gcggttattg ggcacttgaa
     1621 agaaaccaaa gccgagacca cacagcatgg tgagcatgac gagttgcagc gggtgggccc
     1681 gaagcggcca cagcgcgagc gccgcgagtc cgatcgcgag acacacagcg cccgcgacgc
     1741 aaagcctggc cgtcgacacg cggtccgcca gacggcctgc aagcggggcc gcgatagcca
     1801 ctgtcagtgg ccagggcgtc atgtaaagcc cggtcatcca cgcgttcaac ccgagcccgt
     1861 gctgcaggta gaagggcagc gccaccaggc tcgcggccac gccggaaaag cacaggaccg
     1921 aggcaatcac tgaaatccgg aatgaagcgg cacggagcag gtcgagcggg atcagtggca
     1981 cctcctttga catttcgcgc cgaacaagcg tcacaaggct gacggctgct ccgccgacca
     2041 gaacggcggc cagcgccggt cttgccggca ggagttccgc agcgatgacc aatgacgcaa
     2101 aggccgcggc actcagcgcc acgctgcaca ggtccagccg gcgccccgtt ccaccggcct
     2161 cgggtagcgc acgggtggcg aacagcaccg ccacacccag gggcagattg acggcgaaca
     2221 gccagggcca gctcgtcgcg gacaggattg ccgcaccaat cgccggtcct gccgcagacg
     2281 acagcgcgac ggcaagggcg ttccagccga tggcggcacc cagccgttgc tgtgaaacca
     2341 cgacgcgcag cagcgcgagg ccgagcgcca tgacggccgc acccccgagc ccctggagga
     2401 atcgcgccgc gacgagcaac ggcagcgagg gtgacaacgc gcacagcccg gacgcgccaa
     2461 cgaacaacgc aacgccggca gtgaataccc tgcgaaaacc caggctctcc ccgagcgcag
     2521 cacacggcag cagggccatc accagagccg cctggtaggc ggtgatgatc cagaccgatt
     2581 gcgccgacgt cacgcgaagc gatcgcgcga tcgtgggcag ggccacattg gcaatggccg
     2641 cgtccagcac cacgagcacc atggcggcca gcacggaggc gatcgcgacc gcgctgtgca
     2701 atcccctccg gtccggcgcc agacgccgcc ccagcgcatc gacgaacgtg ccgctcatgg
     2761 cgcacctgcc gtccaggcgc cgttgcgctc cacataccgc gtcacccgcc gttcctcctg
     2821 gtcctcccgc ttcaggtcac gctcggtaaa gcgaaacagg agtgccgggc ctttcacgca
     2881 ccgggtatcg tgaatatctc cgggcagata gacgcggacc tgcccggggc gtagcagcgt
     2941 cgaatccctt ttcacgagct cgacattgcc acccgggcgc tcgatccggg cataggttcc
     3001 catctcgatt tcaccctgct
//
LOCUS       DAORUQ010000285         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_45712_length_3019_cov_9.397773, whole genome shotgun sequence.
ACCESSION   DAORUQ010000285 DAORUQ010000000
VERSION     DAORUQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45712_length_3019_cov_9.397773"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLW13_15540"
     CDS             <1..560
                     /locus_tag="PLW13_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQY71842.1"
                     /translation="RVLQEKKVMRLGSDQVIPVDVRVVAATNRNLARLVGEGVFRDDL
                     YYRINVLQLRLPPLRVRPGDIPVLAETFLRRSRCRAGCHHLSPEAIGELAAHPWPGNV
                     RELQNVMERVCATLHEGTITAAFIRRLLEDGPLPAFPAGAPGETDPIARALAQTGGRV
                     AEAARTLGISRSTLWRRMREKELAR"
     gene            584..1189
                     /locus_tag="PLW13_15545"
     CDS             584..1189
                     /locus_tag="PLW13_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQY71843.1"
                     /translation="MTKADESVSLPGATADGKAALRLLLTRYSVSPKHLGEPGPSEDE
                     LWTMAMAALRAPDHNKRIPYRFVVARGDGLERLAALFEDYGRRRGKSGEELARERERA
                     MQAPVVIAVIARIDPHDEEVPPHEQWASVGGAISNALSALHFMGYAGKMVSGLRAADP
                     AIVAAYCGEGETLVGWISAGTPRGPAKPRGEVDADGILSDF"
     gene            complement(1291..1440)
                     /locus_tag="PLW13_15550"
     CDS             complement(1291..1440)
                     /locus_tag="PLW13_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HQY71844.1"
                     /translation="MLYTIAVVLVILWLLGMVTSTTMGGFVHVLLVIAIVVVLLRVIS
                     GRRVL"
     gene            complement(1588..2331)
                     /locus_tag="PLW13_15555"
     CDS             complement(1588..2331)
                     /locus_tag="PLW13_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQY71845.1"
                     /translation="MPTLPNPSQNHLIAALPAQEFERIAAHLEPVALPLGQILYGPGD
                     QLQHAYFPTTAIVSLHYVIESGASAEIASVGNEGVVGISLFMGGDTTPSSAVVHTAGH
                     AYRMERKVLKQEFERGALVQRLLLRYTQALITQMSQTAACNRHHSVEQQLCRWLLVTL
                     DRVPSGRLVMTQELVARMLGVRREGITEAAGRLQEAGCIRYRRGHIEVLGRQELEART
                     CECYRVVRDELRRLLHDPQYRQVESAIEA"
     gene            complement(2354..>3019)
                     /locus_tag="PLW13_15560"
     CDS             complement(2354..>3019)
                     /locus_tag="PLW13_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY71846.1"
                     /translation="RANRYDLILLDLQMPGMDGFQVMEGLKTNEADGYLPVLVITAQP
                     GHKLRALQAGARDFVSKPFDLVEVRTRIRNMLEVRLLYRRLEEFNAALEQKVGERTAE
                     LRESEARYRSLTELASDWYWEQDESGSFTKVSGPVLEMLGLRVDSLAGGPAAAEADGW
                     NVVEREALQARITARQPFLDFPFSRVNADGSESRFRVSGEPMFDQGCRFIGYRGIGVE
                     VSP"
BASE COUNT          458 a         1030 c         1081 g          450 t
ORIGIN      
        1 tgcgcgtgct gcaggagaag aaggtgatgc gcctgggcag cgaccaggtg atccccgtgg
       61 acgttcgcgt ggtggccgcg accaaccgca acctcgcgcg gctggtgggc gagggcgtgt
      121 tccgggacga cctctactac cgcatcaacg tgctgcagct caggctgccg ccgcttcggg
      181 tgcggcccgg ggacattccg gtgctcgccg agactttcct gcggcgctcg cgctgccgcg
      241 ccggctgcca ccatttatcg cccgaggcga tcggcgagct ggccgcgcat ccgtggccgg
      301 gcaacgtgcg cgagctgcag aacgtgatgg agcgcgtgtg cgccacgctg cacgagggta
      361 cgatcaccgc tgccttcatc cggcggctgc tcgaggacgg gccgcttccc gcgtttcccg
      421 ccggcgcacc gggcgagacc gatcccatcg cgcgggcgct cgcgcagacg ggcggacgcg
      481 tggccgaggc cgcgcgcacg ctggggatca gccgctcgac cctgtggcgg cggatgcgcg
      541 agaaggaact ggcacgctga gcgggcgagg ggaccaacag ccgatgacga aagccgacga
      601 gagcgtttcc ctgcccggcg ccacggccga tggcaaggcg gcgctgcgcc tgctgctcac
      661 gcgctattcg gtgagcccga agcacctggg cgagccgggg ccttcggagg acgaattgtg
      721 gacgatggcc atggcggcac tgcgcgcgcc ggaccacaac aagcgcattc cctaccggtt
      781 cgtggtcgcc cgcggcgatg ggctcgaacg cctggcggcg ctcttcgagg actacggccg
      841 gcggcgggga aagtcgggcg aggaactcgc gcgcgagcgc gaacgggcga tgcaggcgcc
      901 cgtggtgatc gcggtgatcg cgcgcatcga tccccatgac gaggaagtgc cgccccacga
      961 gcaatgggcg agcgtgggcg gggcgatcag caatgcgctc tccgcgctgc acttcatggg
     1021 ctatgcgggc aagatggtgt cgggccttcg cgccgcggac cccgcgatcg tcgcggccta
     1081 ctgcggcgag ggcgaaacgc tcgtcggatg gatctcggcc ggcacgccaa ggggaccggc
     1141 caagccgcgg ggtgaagtcg acgcggacgg gatcctttcc gacttctgat cctttccgct
     1201 cgccggttga cgaccgccgc ccgcggccag ccgtggctcg gtcgccggtc tcccgcaagt
     1261 cgatgcgccg atcaccgcgc ccgcgcggtc ctagagaacg cggcgtccac tgatgacgcg
     1321 cagcaggacg acgacgatgg cgatcaccag caggacatgc acgaaaccgc ccatggtggt
     1381 ggacgtgacc atgcccagga gccacaggat gacgagtacg acggcgatgg tgtacagcat
     1441 ggtctctctc cttgacgcat gacacggccg ggtatccggc tgcgcctgcg caaacccatg
     1501 ctgcgccgcg cgctggcccc tgtcggtgcg gtctcgcaca aaggggcgat gccctcccgc
     1561 tgctcgccga aacctcggtc cccgcgctta cgcctcgatg gcggactcga cctggcggta
     1621 ttgcggatcg tggagcaggc ggcgcaactc gtcgcgcacc acgcggtagc actcgcaagt
     1681 gcgggcttcc agctcctggc gcccgagcac ctcgatgtgg ccgcggcggt agcggatgca
     1741 gccggcctcc tggaggcggc ccgccgcctc ggtgatgcct tcgcggcgaa cgccgagcat
     1801 gcgggcgacc agctcctgcg tcatgaccag ccggcccgag ggaacgcgat cgagcgtcac
     1861 caggagccag cggcagagct gctgctcgac cgaatgatgc cggttgcagg ccgcggtctg
     1921 cgacatctgc gtgatgagcg cctgcgtgta gcgcagcagc aggcgctgca ccagcgcgcc
     1981 gcgctcgaac tcctgcttga ggacctttcg ttccatccgg tacgcgtggc ccgcggtatg
     2041 cacgacggcc gagctcggcg tggtgtcgcc gcccatgaag agcgaaatgc cgaccacgcc
     2101 ctcgttgccg acgctcgcga tctccgccga ggcgccggac tcgatcacgt agtgcagcga
     2161 cacgatggcg gtcgtcggaa agtaggcgtg ctgcaactga tcgccgggcc cgtagaggat
     2221 ctggcccagg ggcagcgcca ccggttccag gtgcgcggcg atgcgctcga actcctgcgc
     2281 cggcagggcg gcgatcaggt gattctggct cgggttcggg agcgtcggca tgggggcgag
     2341 tccgcggtcc cggtcagggc gagacttcca cgccgatgcc gcgatagccg atgaagcggc
     2401 agccctggtc gaacatcggc tctccgctca cccggaaccg gctctccgag ccgtcggcgt
     2461 tcacgcggct gaaggggaaa tcgagaaacg gctggcgcgc ggtgatgcgg gcctggagcg
     2521 cttcgcgctc gaccacgttc cagccatcgg cttcggcggc tgccgggccc ccggccagcg
     2581 agtcgaccct gaggccgagc atctccagca cgggccccga gaccttcgtg aagctgccgc
     2641 tctcgtcctg ctcccagtac cagtcggagg ccagttcggt caggctgcga tagcgcgcct
     2701 cgctttcgcg cagctcggcc gtgcgctcgc cgaccttctg ctccagcgcc gcgttgaatt
     2761 cctcgagacg gcggtaaagc aggcgcacct ccagcatgtt gcggatgcgc gtgcgcactt
     2821 ccaccaggtc gaacggcttg gagacgaaat cccgggcgcc ggcctgcagc gcgcgcagct
     2881 tgtggccggg ctgggcggtg atcacgagca cgggcaggta gccgtccgcc tcgttggtct
     2941 tgaggccctc catcacctgg aagccgtcca tgccgggcat ctgcaggtcg agcaggatca
     3001 ggtcgtagcg gttcgcgcg
//
LOCUS       DAORUQ010000286         3003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_46083_length_3003_cov_5.486771, whole genome shotgun sequence.
ACCESSION   DAORUQ010000286 DAORUQ010000000
VERSION     DAORUQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46083_length_3003_cov_5.486771"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PLW13_15565"
     CDS             complement(<1..156)
                     /locus_tag="PLW13_15565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY71847.1"
                     /translation="MVTLHTSMGDITLELDAEKAPKTVANFLQYARDGFYDGTIFHRV
                     INNFMIQG"
     gene            271..1944
                     /locus_tag="PLW13_15570"
     CDS             271..1944
                     /locus_tag="PLW13_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQY71848.1"
                     /translation="MTDAPKPSNFLRTIIRDDLAADRVSGVVTRFPPEPNGYLHIGHA
                     KSICVNFGLAQEFGGVCNLRFDDTNPSTEDVEYVDSIIRDVSWLGYAWDGEVRYASDY
                     FETLYGWAQHLVREGLAYVCELGPEEMRQYRGTLTEPGRNSPWRERPAPESLELLAQM
                     RQGSIEEGRMTLRARIDMGSPNLNLRDPVLYRVKHAHHHRTGNAWHIYPSYDFAHGQE
                     DAIERVTHSICTLEFEDHRPLYEWFIANLPVPSRPRQYEFARLNLSYTVTSKRKLRQL
                     VEEGHVQGWDDPRMPTISGLRRRGVSPGAIRDFVERTGVSRSDGLVDLGMFEYCIRDD
                     LDKNAPRAMCVLRPLKVTLTNYPDAQHDELLVMAHPQRLDLGQRVLPFSRELWIDRED
                     FRMEASKDYKRLVLGAEVRLRNAYVIRADEVVRDAAGEIVELRCSYDPDTLGKNPADG
                     RKVKGVIHWVEASRAIDCEVRLYDRLFRDAQPDAGERSFLDNLNPDSLQVLAGCKAER
                     SLADAALGQTFQFEREGYFCRDPEPAANGQVVFNRTIGLRDVWSEAGKG"
     gene            1948..>3003
                     /gene="cysS"
                     /locus_tag="PLW13_15575"
     CDS             1948..>3003
                     /gene="cysS"
                     /locus_tag="PLW13_15575"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQY71849.1"
                     /translation="MALRIYNSLTRRAEDFVPMVPGRIGMYVCGITVYDYCHIGHARL
                     LVGFDVIARYLRFSGWELDYVRNITDIDDKIIRRADENAEPYDALTARMIAAMHDDER
                     ALGNLPPDREPRATAHIGEILAIVQKLVDTGHAYAAANGDVYYRVMRFAGYGKLSGKN
                     PDELLSGARVEVDEDKEDPRDFVLWKAAKPGEPAWDSPWGAGRPGWHIECSAMSMRCL
                     GETVDIHGGGPDLIFPHHENEIAQSEAATGKCFAHYWMHVGALRVDNEKMSKSLGNFF
                     TIREVLARYRPEVLRFLLISSQYRSPINYAEDSLQLAQAGLERFYAALRDFGDVAPLP
                     AAELAQTPYAARFREAMD"
BASE COUNT          512 a         1008 c         1009 g          474 t
ORIGIN      
        1 gccctggatc atgaaattgt tgatcacgcg gtggaagatc gtgccgtcgt agaagccgtc
       61 gcgcgcgtac tgcaggaagt tggcgaccgt cttcggcgcc ttttcggcgt cgagttcgag
      121 ggtaatgtcg cccatggagg tgtggagagt gaccatctgg atatcccgtg tgcgtgaaaa
      181 aatgaggcgg cattgtaaag ggttttgttg gttggaccta ccccggcggc tatactggcc
      241 cgcttgattt tcccgcgacc cgataccccg atgacagacg cccccaaacc ctcgaacttc
      301 ctgcgcacga tcatccgcga cgacctggcg gcggaccgcg tgagcggggt cgtcacgcgt
      361 ttcccgccgg agcccaacgg ctacctgcac atcgggcacg ccaagtcgat ctgcgtgaac
      421 ttcgggctgg cgcaggagtt cggcggggtc tgcaacctgc gcttcgacga caccaacccc
      481 tccaccgagg acgtcgagta cgtcgattcg atcatccgcg acgtgtcctg gctgggttac
      541 gcctgggacg gcgaggtgcg ctacgccagc gattacttcg agacgctcta cggctgggcg
      601 cagcacctgg tgcgcgaggg gctggcctac gtctgcgagc tggggcccga agagatgcgc
      661 cagtatcgcg gcacgctgac cgagcccggg cgcaacagcc cgtggcgcga gcgcccggcc
      721 cccgagagcc tggagctgct cgcgcagatg cgccagggca gcatcgagga gggccgcatg
      781 acgctgcgcg cgcgcatcga catgggctcg cccaacctga acctgcgcga cccggtgctc
      841 taccgcgtga agcacgcgca ccatcaccgc accggcaacg cctggcacat ctacccgtcc
      901 tatgacttcg cgcacgggca ggaggacgcc atcgagcgcg tgacgcactc gatctgcacg
      961 ctggagttcg aggatcaccg cccgctgtac gagtggttca tcgcgaacct gccggtgccg
     1021 agccgtccgc gccagtacga attcgcgcgc ctgaacctga gctacaccgt caccagcaag
     1081 cgcaagctgc gccagctggt cgaggaaggg cacgtgcagg gctgggacga tccgcgcatg
     1141 ccgacgatct cgggcctgcg ccggcgcggc gtcagcccgg gggcgatccg cgacttcgtc
     1201 gagcgcaccg gcgtgagccg ctccgacggc ctggtcgatc tcggcatgtt cgagtactgc
     1261 atccgcgacg acctcgacaa gaacgccccg cgcgcgatgt gcgtgctgcg tccgctcaag
     1321 gtcacgctca cgaattatcc cgacgcgcag cacgacgagc tgctggtcat ggcgcatccg
     1381 cagcgcctcg atctcggcca gcgggtcctg ccgttctcgc gcgagctgtg gatcgatcgc
     1441 gaggacttcc gcatggaggc ctcgaaggac tacaagcgcc tggtgctggg tgccgaggtc
     1501 aggctgcgca atgcctacgt gatccgcgcc gatgaggtgg tgcgcgatgc cgccggcgag
     1561 atcgtcgagc tacgctgcag ctacgacccg gatacgctcg gcaagaatcc cgccgacggg
     1621 cgcaaggtca agggcgtgat ccactgggtc gaggccagcc gggcgatcga ctgcgaggtg
     1681 aggctctacg accggctgtt ccgcgacgcg cagcccgatg cgggcgagcg cagtttcctc
     1741 gacaacctga accccgacag cctgcaggtg ctcgccggct gcaaggccga gcgcagcctc
     1801 gccgacgcag cgctgggcca gaccttccag ttcgagcgcg agggttattt ctgccgcgat
     1861 ccggagcccg ccgcaaacgg gcaggtggtg ttcaacagga ccatcgggct gcgcgacgtg
     1921 tggtccgagg cggggaaggg ctgagtcatg gcactgcgca tctacaactc gctgacccgt
     1981 cgtgccgagg acttcgtgcc gatggtgccc gggcgcatcg gcatgtacgt ctgcggcatc
     2041 acggtctacg actactgcca catcgggcat gcgcggctgc tggtcggctt cgacgtgatc
     2101 gcgcgctacc tgcgcttcag cggctgggag ctcgattacg tccgcaacat caccgacatc
     2161 gacgacaaga tcatccgccg cgccgacgag aacgccgagc cctatgacgc gctcaccgcg
     2221 cgcatgatcg ctgcgatgca cgacgacgag cgcgcgctgg gcaacctgcc tccggatcgc
     2281 gagccgcgcg ccaccgcgca catcggcgag atcctcgcga tcgtgcagaa actcgtcgac
     2341 acgggccatg cctacgccgc cgccaacggc gatgtgtact accgcgtcat gcgctttgcc
     2401 gggtacggca agctctcggg caagaacccc gacgaattgc tctcgggcgc gcgcgtcgag
     2461 gtcgacgagg acaaggaaga cccgcgcgac ttcgtgctgt ggaaggccgc caagcccggc
     2521 gaaccggcgt gggattcgcc gtggggcgcg gggcgcccgg gctggcacat cgagtgctcg
     2581 gcgatgtcga tgcgctgcct cggggagacc gtcgacatcc acggcggcgg gccggatctc
     2641 atcttcccgc accacgagaa cgagatcgcg cagagcgagg cggccaccgg caagtgcttc
     2701 gcgcactact ggatgcacgt gggggcgctg cgcgtcgaca acgagaagat gtcgaagtcg
     2761 ctcggcaact tcttcacgat ccgcgaggtg ctggcgcgct accgccccga ggtgctgcgc
     2821 ttcctgctga tttccagcca gtaccgcagc ccgatcaact atgccgagga cagtctgcaa
     2881 ctggcgcagg cgggcctgga gcgcttctac gccgcgctgc gcgacttcgg cgacgtggca
     2941 ccgctgccgg ccgcggagct ggcgcagaca ccctatgccg cgcgctttcg cgaggcgatg
     3001 gac
//
LOCUS       DAORUQ010000287         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_46444_length_2987_cov_5.698840, whole genome shotgun sequence.
ACCESSION   DAORUQ010000287 DAORUQ010000000
VERSION     DAORUQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46444_length_2987_cov_5.698840"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLW13_15580"
     CDS             <1..498
                     /locus_tag="PLW13_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQY71850.1"
                     /translation="EYVSVQSQADTEAADMAKERNELETEPERELAELTAIYVGRGLD
                     EPLARQVAERLTAGDALAAHARDELGITETLRARPVQAAFASAAAFCAGAIIPILTAL
                     LAPAARVAEVSSLTSLVALLLFGGLAGYAGGASITKGALRVAFWGAFAMGLTALVGRL
                     FGAAV"
     gene            506..1264
                     /locus_tag="PLW13_15585"
     CDS             506..1264
                     /locus_tag="PLW13_15585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY71851.1"
                     /translation="MFPLYDDNVRLRWPLGTVLIIALNVFAWFFVQGFGVERPLARSL
                     CLHGLIPADLLGLVPAGFQVPLGPRLACVVDGGGSWFNLFSSMFMHGGWFHIIGNMWF
                     LWVFGDNVEDVMGLPRFLLFYLLSGLAAAGAQIATDPSSTVPMVGASGAIGGVLGAYA
                     RMYPRAHVHMLIFLGFYITTVAVPAMFMLGYWFLLQLLSGTLGQGGSGVAFWAHIGGF
                     VAGLLLSLVLVSPRRLDEHRSAQRRDLHSRYRWF"
     gene            complement(1249..>2987)
                     /locus_tag="PLW13_15590"
     CDS             complement(1249..>2987)
                     /locus_tag="PLW13_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71852.1"
                     /translation="ADTDFLADGVSEELRDTLARYQGLLVTAHASAFRLQGSQTDVRE
                     LGRQLGVRYLLQGSVRKIDERVRIDARLTEAASGRQLWSGNYERTLAQLLAVQQEIAT
                     EVARALELVPVSAQTGTAGARTPNVEAHLEFLRARERLATWAVDDAEQAIAHLQRAIA
                     LDPGYAAAYAQLADAIMIRALSTGGVEPVRATVEPLLDKALALDPGLGEAYVTRSWLI
                     EDRAQEEREVRKGLELSPNYARGYEMLAARLMELPERQAEALRLIDRALALDPLTPRN
                     HHIKAIFLAQLGDLDGAEELERRALGIDPRFRSAQLWLGTLSSLRGEFAQAVEHGERA
                     LAIDPRATFLREGLVNFYLSLGDAAAADAIDSPATPNGRLALLLFDADWNGAADWIDR
                     AEGSATRPELAKHLVSYARLRRGLASGSPAQAATALAAELGFDGTLPRLTAAPEFRAY
                     TDLGQLLHASGDEAAAKRVQQLLEAELDELEAVAPGQKRALDAVRALLLAQAGRDRDA
                     FAALERAYAAAPLPLWWIHLKHPAFERLRGQPRFQALVARVDAHLEAQREKLAQMRQR
                     GLVPERAVQNQR"
BASE COUNT          380 a         1041 c         1064 g          502 t
ORIGIN      
        1 gagtacgtgt cggtgcaatc gcaggccgac accgaggccg cggacatggc caaggaacga
       61 aacgagctgg aaaccgagcc cgaacgcgaa ctcgcggaac tcaccgcgat ctacgtcggc
      121 cgtggactcg acgagccgct ggcgcgccag gttgccgaga ggctcaccgc gggcgatgcg
      181 ctcgccgcgc acgcgcgcga cgagctcggc atcaccgaga cgctgcgcgc gcgcccggta
      241 caggccgcct tcgcctccgc cgcggccttc tgcgccgggg ccatcattcc catcctgacg
      301 gcgctgctcg cgcccgccgc gcgggtcgcc gaggtttcgt ccctgacctc gctcgtggcg
      361 ttgctgctct tcggcggact ggcggggtat gcgggcgggg catcgatcac gaagggcgcc
      421 ttgcgggtcg cgttctgggg agcattcgcc atgggactca cggcgctcgt cgggcggctg
      481 ttcggcgcgg cggtgtagcc gccgcatgtt tccgctctac gacgacaatg tgcggctgcg
      541 ctggcccctg ggcaccgtgc tgatcatcgc gctcaacgtc ttcgcgtggt tcttcgtgca
      601 gggtttcggt gtcgaacgcc cgctggcgcg ctcgctgtgc ctgcacgggc tgattccggc
      661 ggatctgctg gggctggtgc cggcgggctt ccaggtgccg ctcggtcccc ggcttgcctg
      721 cgtggtcgac ggtggcggca gctggttcaa cctgttcagt tcgatgttca tgcacggcgg
      781 ctggttccac atcatcggca acatgtggtt cctgtgggtg ttcggggaca acgtcgagga
      841 cgtcatgggg ttgccgcgct tcctgctgtt ctacctgctg tcgggactgg cggccgccgg
      901 cgcgcagatc gcgacggatc catccagcac cgtgccgatg gtcggcgctt cgggcgccat
      961 cggcggcgtg ctgggcgcgt atgcgcgcat gtatccgcgc gcacacgtgc acatgctgat
     1021 cttcctgggc ttctacatca ccacggtcgc ggtgccggcg atgttcatgc tcggctactg
     1081 gttcctcctg cagctgctga gcggcacgct gggccagggc ggttcggggg tggcgttctg
     1141 ggcgcacatc ggcggtttcg tggcgggctt gctgctgtcg ctggtgctgg tcagcccacg
     1201 ccgcctggac gagcatcgca gcgcgcagcg ccgcgacctg cactcgcgct accgctggtt
     1261 ctgaacggcg cgctcgggaa cgagcccgcg ctggcgcatc tgcgccagtt tctcgcgctg
     1321 tgcctccagg tgcgcgtcca cgcgcgcgac gagcgcctgg aagcgcggtt gtccgcgcag
     1381 gcgctcgaac gccggatgct tcaggtgaat ccaccacagc ggcagcggcg ccgccgcgta
     1441 ggcgcgctcc agcgcggcga aggcgtcccg gtcgcggccc gcctgcgcca gcagcagggc
     1501 acggacagcg tccagcgcgc gtttctggcc gggcgccacc gcttccagct catccagttc
     1561 ggcctcgagg agttgttgca cgcgcttcgc ggcggcctcg tcgccgctgg catgcagcag
     1621 ctgcccgagg tcggtgtagg cgcggaattc cggggccgcc gtgagccggg gaagggtgcc
     1681 gtcgaagccg agctccgctg cgagcgccgt ggctgcctgc gccgggctgc cagaggcgag
     1741 gccgcggcgc aggcgcgcgt aggacacgag atgctttgcg agctccgggc gggtcgcgga
     1801 accctccgca cgatcgatcc agtccgccgc cccgttccag tcggcatcga acagcagcag
     1861 cgcgagccgg ccgttcggtg ttgcggggct gtcgatggcg tcggcggccg cggcatcgcc
     1921 gaggctcagg tagaaattga ccagcccttc gcgcaggaat gtcgcgcgcg ggtcgattgc
     1981 cagcgcgcgt tccccgtgtt ccacggcctg cgcgaactcg ccgcgcagcg agctcagcgt
     2041 ccccagccaa agctgcgccg agcgaaagcg cggatctatc ccgagcgcgc gacgctccag
     2101 ttcctcggcg ccgtccaggt cgcccagctg cgcgaggaaa atcgccttga tgtgatgatt
     2161 gcgcggcgtc agcggatcca gcgccagcgc gcgatcgatc agccgcagcg cctcggcctg
     2221 acgttcgggc agctccatca accgcgcggc cagcatctcg tacccacgcg cgtagttggg
     2281 gctgagctcc aggcccttgc gcacttcgcg ttcctcctgc gcgcgatcct cgatcagcca
     2341 gctgcgcgtc acgtaggctt cgcccaatcc ggggtccagc gccagcgcct tgtccagcag
     2401 cggttccacg gtggcgcgca cgggttcgac accgccggtc gagagcgcac ggatcatgat
     2461 cgcgtcagcc agttgcgcgt aggccgcggc atagccggga tcgagcgcga tggcgcgctg
     2521 caggtgcgcg attgcctgtt ccgcgtcgtc caccgcccag gtggcgagtc gctcgcgcgc
     2581 gcgcaggaac tccaggtgcg cctcgacgtt cggggtgcgc gccccggcag tgccggtctg
     2641 cgccgagacc ggcaccagct cgagcgcgcg cgcgacctcc gtggcgatct cctgctgcac
     2701 ggcgagcagc tgcgccagcg tgcgctcgta attgccgctc cagagttgcc tgccggaggc
     2761 ggcctcggtg agccgggcgt cgatgcgcac gcgctcatcg attttccgca cgcttccctg
     2821 cagcaggtag cgcacgccga gttgtcggcc gagctcgcgc acgtcggttt gcgagccctg
     2881 caaccggaac gccgaggcgt gcgccgtgac cagcaggccc tggtagcgcg ccagcgtgtc
     2941 acgcagttcc tcggacacgc cgtccgcgag gaagtcggtg tcggctt
//
LOCUS       DAORUQ010000288         2974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_46790_length_2974_cov_4.575197, whole genome shotgun sequence.
ACCESSION   DAORUQ010000288 DAORUQ010000000
VERSION     DAORUQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46790_length_2974_cov_4.575197"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PLW13_15595"
     CDS             <1..110
                     /locus_tag="PLW13_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQY71853.1"
                     /translation="EKLKYDLYYVKHRSFLLDLNILVQTVEVVLFGKGR"
     gene            complement(236..1588)
                     /gene="prsR"
                     /locus_tag="PLW13_15600"
     CDS             complement(236..1588)
                     /gene="prsR"
                     /locus_tag="PLW13_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HQY71854.1"
                     /translation="MQDSDKHLLIVEDDEGLQRQLRWCFDGYAVTVAGDRKDAIARVR
                     RHQPGVVLLDLGLPPDPGGVTEGLATLQEIQGLSPETKVIIVTGDNERANAVRAIALG
                     AYDFYQKPVEPEVLTLMVDRAYRLHELELENRRLQQAHASEPMNGIIATSPQMLKVCR
                     TIEKVAPSDVTVLLLGASGTGKERVAQALHERSPRAKQRMVAINCAAIPENLLESELF
                     GYEKGAFTGAVKQTQGKIEYANGGTLFLDEIGDLPLALQSKLLRFLQERVIERVGGRE
                     EIPINVRIICATHQDLQGKIADGSFREDLYYRISEITIKIPAVKDRDGDALVLAKSFL
                     NRFCQEQGRTIRGFDRDATAAIETHAWPGNVREIESRVKRAIIMADGTYITPDDLELD
                     VVETESTPLNLKQVREEAELRAIQRALVSADDNISEAAKLLGVTRPTLYSLVEKYNLK
                     "
     gene            complement(1613..>2974)
                     /gene="prsK"
                     /locus_tag="PLW13_15605"
     CDS             complement(1613..>2974)
                     /gene="prsK"
                     /locus_tag="PLW13_15605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HQY71855.1"
                     /translation="TVFGAGSYLLLMATVGYFIRFYGGSWGGVLQIAFLFGTGLLLVV
                     LLFSDKIRRRLRVFLNKHFFSYKYNYREEWLKFTQSLAETTEEVPERVVRAVATLVSS
                     PGGMLWEFSALGHARLLTRWNMPEPQPGQPVDLESLAHFLESTGWVIDLEEYARSPAL
                     YEGLVLPDWCRRLPGAWLLVPLLFRARAIGCVLLARSDVHGAINWEDRDLLKTAGLQA
                     AGQLAQYQSDRALVQAQQFEAFNRLSAYVVHDLKNILAQQSLIVSNAEKHKHKPEFVD
                     DVIDTVRNSVARMTRLMEQMRSGSRGGPTQSVSLQAVLATAVARRAHQLPVPELEAAD
                     EGLVVQADREGLATVFGHLLQNAQEATEKQGRVTVRLLREEGWAVVEIEDTGCGMDAD
                     FIRERLFKPFDSTKGLTGMGIGAYESREFIRALGGEIRVNSAPGTGSIFRVLLPCHDA
                     QGA"
BASE COUNT          450 a          976 c         1004 g          544 t
ORIGIN      
        1 tggagaagct caaatacgac ctctactacg tgaagcaccg cagcttcctg ctggatctga
       61 acatcctggt gcagaccgtg gaggtggtgt tgttcggaaa ggggcgttaa ccgtaggtcc
      121 ggatttatcc gggcaatgtg atacccggtt ttggccacgt aggtccgcat tcatgcggac
      181 ttaaccacat ccctcatgtc cctcatgtcc gcatgaatgc ggacctacgg gggggttatt
      241 tgaggttgta tttttcgacg aggctgtaga gggtggggcg ggtcacgccg agcagcttgg
      301 cggcttcgga gatgttgtcg tcggcgctga ccagggcgcg ctggatggcg cgcagttcgg
      361 cttcctcgcg cacctgcttg aggttcagcg gcgtcgattc cgtctcgacg acgtcgagct
      421 cgaggtcatc gggtgtgatg taggtgccgt cggccatgat gatcgcgcgc ttcacgcggc
      481 tttcgatctc gcgcacgttg ccgggccagg cgtgggtctc gatggccgcg gtggcgtccc
      541 tgtcgaagcc gcggatcgtg cggccctgct cctggcagaa gcggttcagg aaggacttgg
      601 ccagcaccag cgcgtcgccg tcgcgatcct tcacggccgg gatcttgatc gtgatctcgc
      661 tgatgcggta gtacaggtcc tcgcggaagc tgccgtcggc gatcttgccc tgcaggtcct
      721 ggtgcgtggc gcagatgatg cgcacgttga tcgggatttc ctcgcgcccg ccgacgcgct
      781 cgatcacgcg ctcctgcagg aagcgcagca gcttcgactg cagcgccagt ggcaggtcgc
      841 cgatctcgtc caggaacagg gtgccgccgt tggcgtactc gatcttgcct tgggtctgct
      901 tgaccgcgcc ggtgaaggcg cccttctcgt agccgaacag ttcgctctcg agcaggttct
      961 cgggaatcgc ggcgcagttg atggccacca tgcgctgttt ggcgcggggg ctgcgctcgt
     1021 gcagcgcttg cgccacgcgc tccttgccgg tgccgctggc gccgagcagc agcacggtga
     1081 cgtccgaggg tgccaccttc tcgatggtgc ggcagacctt cagcatctgc gggctggtgg
     1141 cgatgatgcc gttcatcggc tcgctggcgt gtgcctgctg caggcggcgg ttctcgagtt
     1201 cgagctcatg gagccggtag gcacggtcga ccatcagcgt gagcacttcg ggctccaccg
     1261 gcttctggta gaaatcgtag gcgccgaggg caatggccct gaccgcgttg gcgcgctcgt
     1321 tgtcaccggt gacgatgatc accttggtct cgggcgagag gccctggatt tcctgcaggg
     1381 tcgccagccc ctcggtcacg ccgccggggt cgggggggag gcccaggtcg agcagcacca
     1441 cgccgggttg gtgacggcgc acgcgcgcaa tggcgtcctt gcgatcaccc gccacggtca
     1501 ccgcgtaacc gtcgaaacac cagcgcaact ggcgctgcag gccctcgtcg tcctcgacga
     1561 tcagcagatg cttgtccgaa tcctgcacgc actctctccc gcgactgtcg atctacgcgc
     1621 cctgcgcgtc atgacagggt agcagcaccc ggaagatcga gccggtcccg ggcgcgctgt
     1681 tcacgcgaat ttcgccaccc agcgcacgaa tgaactcgcg gctctcgtag gcgccgatgc
     1741 ccatgccggt caggcccttg gtggaatcga agggcttgaa caggcgctcg cggatgaaat
     1801 cggcatccat gccgcagccg gtatcctcga tctcgaccac ggcccagccc tcctcgcgca
     1861 gcagccgtac cgtgacgcgc ccctgctttt ccgtggcttc ctgcgcgttc tgcagcaggt
     1921 ggccgaacac ggtggccagc ccctcgcggt cggcctgcac cacgagaccc tcgtcggcgg
     1981 cttccagctc cggcaccggg agctggtgcg cgcggcgggc cacggcggtc gccagcacgg
     2041 cctgcaggct cacgctctgc gtcgggccgc cgcgcgagcc gctgcgcatc tgctccatca
     2101 ggcgcgtcat gcgcgccacg gagttgcgca cggtgtcgat gacgtcgtcg acgaattccg
     2161 gcttgtgctt gtgcttctcg gcgttggaga cgattagcga ctgctgcgcc aggatgttct
     2221 tcagatcgtg caccacgtag gccgagagcc ggttgaaggc ctcgaactgc tgcgcctgca
     2281 ccagggcgcg gtcggactgg tactgcgcca gctgcccggc ggcctgcagg ccggcggtct
     2341 tcagcaggtc gcggtcctcc cagttgatgg ccccgtgcac gtcggagcgg gcgagcagca
     2401 cgcagccgat ggcgcgcgcg cggaacagca gcggcaccag cagccaggcg ccgggcagcc
     2461 ggcggcacca gtcgggcagc accagccctt cgtagagcgc ggggctgcgc gcgtattcct
     2521 cgaggtcgat gacccagccg gtgctttcca ggaagtgcgc cagcgattcc aggtccacgg
     2581 gctgacccgg ttgcggctcc ggcatgttcc agcgcgtcag caggcgcgcg tgcccgagcg
     2641 ccgagaattc ccacagcatc ccgcccgggc tgctgaccag ggtcgccacc gcacgcacca
     2701 cgcgctcggg cacttcctcg gtggtttcgg ccagggactg ggtgaacttc agccattcct
     2761 cgcggtagtt gtacttgtag ctgaagaagt gcttgttcag gaacacgcgc aggcggcggc
     2821 ggatcttgtc cgagaacagc agcaccacca gcagcaggcc cgtgccgaac aggaaggcga
     2881 tctgcagcac gccgccccag ctgccgccgt agaaccggat gaagtagccc accgtggcca
     2941 tcagcagcag gtagctgccc gccccgaaca ccgt
//
LOCUS       DAORUQ010000289         2966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_46979_length_2966_cov_5.694263, whole genome shotgun sequence.
ACCESSION   DAORUQ010000289 DAORUQ010000000
VERSION     DAORUQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46979_length_2966_cov_5.694263"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLW13_15610"
     CDS             complement(<1..1080)
                     /locus_tag="PLW13_15610"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQY71856.1"
                     /translation="MTDTHILVINSGSSSLKFSVHDMRGEREIAAGKIEHIGEDGAGP
                     ADHRAALAQALAGIAEQDAIVAVGHRVVHGGERFLAPVRMDAAALAELARFDALAPLH
                     NPANRLGIALGMELLPGRTHVAVFDTAFHHSLPPAAMHYAVPAQWRARGVRRYGFHGT
                     SHRYVSREAARMLGKTATETNLISLHLGNGCSVAAVRGGLSVDTSMGMTPLEGLVMGT
                     RPGDIDAGALLHLLREGMDPRELDEALHHHSGLRGLCGENDMRAVHALAATGNADAAL
                     ALEMYCYRARKYLGAYGAVLGRVDALVFTAGIGEHDADIRARVLAGLEWFGVRLDAER
                     NRACRGERAMAIHAPSSRVAILVIPT"
     gene            complement(1187..2770)
                     /locus_tag="PLW13_15615"
     CDS             complement(1187..2770)
                     /locus_tag="PLW13_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71857.1"
                     /translation="MTESVFALACARAPEGMEISAVAAAQPGRIALWSDQGTMSFAEL
                     NAQANRLVRRMRAAGLAAGDAVALVCSNRTEFAVVRFATHRAGLRLTPINWHLAPEDI
                     RYIVENAEAKALFLDTRVAAVAPGCAQIPRLRLRVAIGGELPDYTSWADALAGMDCSD
                     IPDPSLGATMLYTSGTTGRPKGVYRPPLDPVHAAQLQQMLTAVFQFDPESGTDRALAT
                     GPLYHTGPFALCFTTPLTAGIGAVIMDKWDPQRMLALIAQYRITHTFCVPTMFNRLLQ
                     LPDEVRARHDVSSLRFVIHGAAPCAVDVKKRMLDWWGPILWEMFAGTEGQGTIVSPQE
                     WLAKPGTVGRPAPGQLRILDDAGNELAPGQPGTIYLVNPSGSAFEYYKDPAKTAAVLR
                     EGYFTAGDIGYLDADGYLFLSGRSAEVIISGGVNIYPQEIDDVLAQHRAVADVACVGV
                     PNEDLGEEIKAVVELRPGFAPDAALRQSLTDFCVEHLAKQKWPRSFDFVAALPRSEAG
                     KVLRRALRERYWAGRERQI"
     gene            complement(2775..>2966)
                     /locus_tag="PLW13_15620"
     CDS             complement(2775..>2966)
                     /locus_tag="PLW13_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQY71858.1"
                     /translation="RIAANAPLALAAIKRTVIDSHTESWNEAFTREAQESARVMMSRD
                     AREGPRAFKEKRAPEFKGE"
BASE COUNT          409 a         1035 c         1067 g          455 t
ORIGIN      
        1 ggtcggtatc accaggatcg cgacgcgcga tgacggcgca tggattgcca tggcccgctc
       61 cccgcggcag gcgcggttgc gctcggcgtc gaggcgcacg ccgaaccatt cgagccccgc
      121 gagcacgcgg gcgcggatat cggcgtcgtg ctcgccgatc ccggcggtga acaccagtgc
      181 atccacgcgt ccgagcaccg caccgtaggc gccgaggtac ttgcgcgcgc ggtagcagta
      241 catctccagc gccagcgcgg catcggcgtt gcccgtcgcg gccagcgcgt gcaccgcgcg
      301 catatcgttc tcgccgcaca gcccgcgcag gcccgagtgg tggtggagcg cctcgtcgag
      361 ctcgcgcggg tccatgccct cgcgcagcag gtgcagcagc gccccggcgt cgatgtcgcc
      421 ggggcgcgtg cccatcacca ggccctccag cggcgtcatg cccatcgagg tgtcgacgga
      481 gagcccgccg cgcacggccg cgacgctgca gccgttgccc aggtgcaggc tgatgaggtt
      541 ggtttccgtc gccgtcttgc cgagcatccg cgccgcctcg cggctcacgt agcggtgcga
      601 ggtaccatgg aagccgtagc ggcgcacgcc gcgcgcgcgc cactgcgcgg gcaccgcgta
      661 gtgcattgcc gccggcggca ggctgtggtg gaaggcggta tcgaacaccg cgacgtgcgt
      721 tcgcccgggc agcagctcca tgccgagcgc gatgccgagc cggttggccg ggttgtgcag
      781 cggcgccagc gcatcgaagc gcgcgagctc cgcgagagcg gctgcgtcca tgcgcaccgg
      841 cgccaggaag cgctcgccgc catgcaccac gcggtgcccg acggcgacga tggcgtcctg
      901 ctccgcgatg cctgcgagcg cctgcgccag cgcggcgcgg tgatccgcgg gcccggcccc
      961 gtcctcgccg atatgctcga tcttgcccgc ggcaatctcc cgctcgccgc gcatgtcatg
     1021 cacgctgaac ttcagcgagg aactgccgct gttgatcacc aggatgtgcg tgtcggtcat
     1081 gtagaggcca tcgcgggtac agcttggcgc cgcattgtgg gtcgcgatta tgacagacag
     1141 gccgtcgcgc gcggcggccc tgcgcgtatc ggcgtgacgc gggtgctcaa atctggcgct
     1201 cgcgaccggc ccagtagcgc tcgcgcagcg cgcggcgcag caccttgccg gcttccgagc
     1261 gcggcagcgc cgcgacgaag tcgaagctgc gcggccattt ctgcttcgcc aggtgctcga
     1321 cgcagaaatc cgtgagcgac tgcctgagcg ccgcgtcggg cgcgaagccc gggcgcagtt
     1381 cgaccacggc cttgatctcc tcgccgagat cctcgttcgg cacgccgacg caggccacat
     1441 cggccaccgc gcggtgctgc gcgagcacgt cgtcgatctc ctgaggatag atgttcacgc
     1501 cgccggagat gatcacctcg gcgctgcgcc cgctgaggaa caggtagccg tcggcgtcga
     1561 ggtagccgat gtcgccggcg gtgaagtagc cctcgcgcag caccgcggcg gtcttggcgg
     1621 gatccttgta gtactcgaag gcgctgccgc tcgggttcac caggtagatg gtgccgggct
     1681 gtcccggtgc caactcgttg ccggcgtcgt cgaggatgcg caactgcccc ggcgcgggcc
     1741 tgcccacggt gccgggtttg gccagccact cctgcgggct cacgatggtg ccctggccct
     1801 cggtgccggc gaacatttcc cacaggatcg ggccccacca gtcgagcatg cgcttcttca
     1861 catcgaccgc gcagggcgcg gcgccgtgga tcacgaagcg caggctcgac acgtcgtggc
     1921 gcgcgcgcac ctcatcgggc agctgcaaca gccggttgaa catcgtgggg acgcagaagg
     1981 tgtgggtgat gcggtactgc gcgatcagcg ccagcatgcg ttgggggtcc cacttgtcca
     2041 tgatcacggc gccgatgccg gcggtgagcg gcgtggtgaa gcacagcgca aaggggccgg
     2101 tgtgatacag cggtccggtg gccagcgcgc ggtccgtgcc gctctcggga tcgaactgga
     2161 acaccgcggt cagcatctgc tgcagttgcg cggcgtgcac cgggtccagc ggcggacggt
     2221 acacgccctt gggccgcccc gtggtgcccg aggtgtagag catggtggcg ccgagcgagg
     2281 ggtcggggat gtcgctgcaa tccatgccgg cgagcgcatc cgcccagctc gtgtaatccg
     2341 gcagctcgcc gccgatggcg acgcgcaggc gcaggcgcgg gatctgcgcg cagcccggcg
     2401 cgacggcggc cacgcgcgtg tcgaggaaca gagccttcgc ctcggcgttc tccacgatgt
     2461 agcggatatc ctccggcgcg aggtgccagt tgatcggcgt gagccgcagg cccgcgcgat
     2521 gggtggcgaa gcgcaccacc gcgaactcgg tgcggttgct gcacaccagt gccacggcgt
     2581 cgccggcggc gagtcccgcc gcgcgcatgc gccgcaccag gcggttcgcc tgcgcgttca
     2641 gttcggcgaa actcatcgtg ccctggtccg accacagcgc gatgcgcccg ggctgcgcgg
     2701 cggccaccgc gctgatttcc atgccctcgg gggcgcgcgc gcaggcgagc gcgaacactg
     2761 actcggtcac tgttttactc tcccttgaat tcgggtgcgc gcttttcctt gaaggcgcgc
     2821 ggtccctcgc gggcatcgcg cgacatcatg acccgggccg attcctgcgc ctcgcgcgtg
     2881 aaggcctcgt tccacgactc ggtgtggctg tcgatcacgg tgcgcttgat cgcggcgagc
     2941 gcgagcgggg cgttcgccgc gatgcg
//
LOCUS       DAORUQ010000290         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_47035_length_2964_cov_5.930560, whole genome shotgun sequence.
ACCESSION   DAORUQ010000290 DAORUQ010000000
VERSION     DAORUQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47035_length_2964_cov_5.930560"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1411
                     /locus_tag="PLW13_15625"
     CDS             68..1411
                     /locus_tag="PLW13_15625"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQY71859.1"
                     /translation="MHALDTFLQQNPDIDVFEVLLPDINGQLRGKWVGRENLAKVFSG
                     GFKLPVSTVAFDIWGRDIGATVFEDGDADGVCLPEPATLARVPWLARPTGQVLVSMGT
                     VHAAAYAGDPRTVLSGVLARYAALGLRPVVAIELEFYLFDRERNADGTPRHTQTTSGG
                     REPVGGQTYGIEAMQEMSELMHGVREAALTQALPVDTLIAEAAPSQFEINLFHQDDAL
                     RACDHALLLKRAIKGVARGQGRLASFMAKPFGALAGNGMHIHFSLVDASGRNVFDNGT
                     DQGSELMRHAVAGCLATMSEAMALFAPNFNSYRRFRLGSHAPHAPTWGYENRSAAVRI
                     PGGSRSAIRLEHRVCGADANPYLAVAGMLAGALMGIEQRLAAPAPVAGDAYSQQPAGL
                     PRFWPEALRVFEKSAFVGEYLGEELRRVFLMSKWQELEEFIGHVSPMEYDAYLDL"
     gene            1448..>2964
                     /locus_tag="PLW13_15630"
     CDS             1448..>2964
                     /locus_tag="PLW13_15630"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HQY71860.1"
                     /translation="MPLLQLTDEQTRSWTRAQKDRWWLDNVYRGDMAQLTLRSGLTGF
                     LLGGVLAATALYIGGKTGISIGVGLTSVILAFAIFRTLAGVGLVRDMTILENNCTQSI
                     ATAAGYMITPLISSLAAYMLVTGRIVPWWQMVVWTVVISILGVLVAFPMKRRFINEDQ
                     LPFPEGRACGVVLDSLYTGHADEGMFKARLLALSGGLAAFYQFVSSDGWMRLVQFKLL
                     RMDRWAGMQEPWHFIERLDSYYYLYADKLGWWIPRILGTDVRQLGLRFTLDAAMLGVG
                     GLMGIRVATSCLLGALVNYAVLAPIMIQRGDIREYTSPAGVLIPISRGEIVNQWSLWW
                     AVTMMVVGSLVSLLAKPELFTGALKSLRRDSTQPRGPDVLGHIELPLWMSFVGVPVFS
                     VLGAWVTHVFFGVPWLLAFVSLPLIFVLTVICTNSMALTSWTPTGALSKITQFTMGAI
                     DRANPASNLIPAGMTAEIASNAANLLSDIKPGYMLGAKPRQQAIGHVIGIFAGALT"
BASE COUNT          440 a         1018 c          992 g          514 t
ORIGIN      
        1 tgcctgaggc ggccgggcgg ccggctcccg agcgttcacg attcgcaccc tgatcacggt
       61 tgccgccatg catgcactcg acaccttcct gcaacagaat ccggacatcg atgtcttcga
      121 ggtgttgctg cccgacatca acgggcagtt gcgcggcaag tgggtgggcc gcgagaacct
      181 cgcgaaggtc ttcagcggtg gcttcaagct gccggtctcg acggttgcct tcgatatctg
      241 gggccgtgac atcggcgcca cggtgttcga ggacggcgat gccgacggcg tgtgcctgcc
      301 ggagcccgcg acgctggcgc gcgtgccgtg gctggcacgc cccacgggac aggtgctggt
      361 gtcgatgggc accgtgcacg cggcggccta cgccggcgac ccgcgcaccg tgctttccgg
      421 cgtgctggcg cggtacgcgg cgctgggcct gcgcccggtg gtcgccatcg agctcgagtt
      481 ttacctgttc gaccgcgaac gcaatgccga cggcacgcca cggcacacgc agaccaccag
      541 cggcgggcgc gaacccgtcg gtggccagac ctacggcatc gaggcgatgc aggaaatgag
      601 cgagctcatg catggcgtgc gcgaggcggc gctcacgcag gcgctcccgg tcgatacgct
      661 gattgccgaa gccgcgccct cgcagttcga gatcaacctc tttcaccagg acgacgccct
      721 gcgcgcctgc gaccacgccc tgctgctcaa gcgcgcgatc aagggcgtgg cgcgcgggca
      781 gggccggctc gcctcgttca tggccaagcc cttcggcgcg ctggccggca atggcatgca
      841 catccatttc agcctggtcg acgcgagcgg gcgcaacgtc ttcgacaacg gtaccgacca
      901 gggcagcgag ctgatgcgcc acgccgtggc cggctgtctc gccacgatgt ccgaggcgat
      961 ggcgctgttc gcgccgaact tcaactccta ccgccgcttc cgtctcggtt cgcatgcccc
     1021 gcacgcgccg acctggggtt acgagaaccg cagcgccgcg gtgaggattc cgggtggttc
     1081 gcgcagcgcg atacgcctgg agcaccgcgt gtgcggcgcc gacgcgaatc cctacctcgc
     1141 ggtggccggc atgctggccg gcgcgctgat gggcatcgag cagcggctcg ccgcgccggc
     1201 gccggtggca ggcgacgcct acagccagca gccggcgggg ttgccgcggt tctggcccga
     1261 ggcgctgcgc gtcttcgaga aatccgcctt cgtcggcgag tatctcggcg aggaactgcg
     1321 ccgcgtgttc ctgatgtcga aatggcagga gctcgaggag ttcatcggcc acgtctcccc
     1381 catggaatac gatgcctacc tcgacctgta ggagcgggcc atgcccgcga ccagctgaga
     1441 cctgcccatg ccactgctgc agctgaccga cgaacagacc cgcagctgga cccgcgcgca
     1501 gaaggaccgg tggtggctcg acaacgtcta ccgcggtgac atggcgcagc tcacgctgcg
     1561 ctcgggactc accggcttcc tgctcggcgg cgtgctcgcc gccaccgcgc tctacatcgg
     1621 cggcaagacc ggcatttcga tcggcgtggg gctcacctcg gtgatcctcg ccttcgcgat
     1681 cttccgcacc ctggccggcg tgggcctggt gcgcgacatg acgatcctcg agaacaactg
     1741 cacgcaatcg atcgcgaccg ccgccggcta catgatcacg ccgctgatat cgagccttgc
     1801 cgcctacatg ctggtgaccg ggcgcatcgt gccctggtgg cagatggtgg tgtggaccgt
     1861 ggtgatctcc atactcggcg tgctggtggc ctttccgatg aagcgtcgct tcatcaacga
     1921 ggaccagctg ccgtttccgg aaggccgcgc ctgcggcgtg gtgctggact cgctctatac
     1981 cgggcacgcc gacgagggca tgttcaaggc acgcttgctc gcgctcagcg gcgggcttgc
     2041 cgcgttctac cagttcgtct ccagcgacgg ctggatgaga ctcgtgcagt tcaagctgct
     2101 gcgcatggac cgctgggccg gcatgcagga accgtggcac ttcatcgagc gactcgacag
     2161 ctactactac ctctacgccg acaagctcgg gtggtggatc ccgcgcatcc tcggcaccga
     2221 cgtgcgccag ctcgggctgc gcttcacgct cgatgccgcc atgctgggcg tgggcgggct
     2281 gatgggcata cgcgtcgcca cgagctgcct gctcggcgcc ctggtcaatt acgcggtgct
     2341 ggcgccgatc atgatccagc gcggcgacat ccgcgagtac acctcccccg cgggtgtgct
     2401 gatcccgatc tcgcgtggcg agatcgtcaa ccagtggtcg ttgtggtggg ccgtcacgat
     2461 gatggtggtc ggctcgctgg tgagcctgct cgccaagccg gagctgttca ccggcgcgct
     2521 gaagtcgctg cgccgcgatt caacgcagcc ccgcggcccc gacgtgctcg gacacatcga
     2581 gttgccgctg tggatgtcgt tcgtcggggt gccggtgttc agcgtgctgg gcgcctgggt
     2641 cacgcacgta ttcttcggcg tgccctggct gctggctttc gtgtcgctgc cgctgatctt
     2701 cgtgctcacc gtcatctgca ccaactcgat ggcgctgacc tcctggacac ccaccggcgc
     2761 gctgtcgaag atcacgcagt tcacgatggg cgcgatcgat cgcgccaatc cggccagcaa
     2821 cctgataccc gccggcatga cggccgagat cgcctcgaat gcggcgaacc tgctgtcgga
     2881 catcaagccc ggctacatgc tcggcgccaa gccgcgccag caggcgatcg ggcacgtgat
     2941 cggcatcttt gccggtgcgc tcac
//
LOCUS       DAORUQ010000291         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_47387_length_2951_cov_5.265193, whole genome shotgun sequence.
ACCESSION   DAORUQ010000291 DAORUQ010000000
VERSION     DAORUQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47387_length_2951_cov_5.265193"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2951)
                     /locus_tag="PLW13_15635"
     CDS             complement(<1..>2951)
                     /locus_tag="PLW13_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQY71861.1"
                     /translation="GRHQNELTRFRYDVFVHVESRGGAAPPPSWLDWREDGLRLADLQ
                     ERLASGPPVLGVRGVPNARLEAAAKALDWLAGGEDGPQTLEGFRQAIAAQPGEALEPE
                     ALCSIAEEHGYGVELGYAGGGSKADMDVLYRKRGTVRPGEVFWAQPANRVRKPWAAYA
                     NNPLKAKLVRDLTPRLRDYLAEALPEYMVPSAIVVLDELPLTPNGKVDRRALPAPGRA
                     RLKAAYVAPRTRNEEILSGIWAEVLKLDRLGVEDDFFDLGGHSLLAMQVVSRMRQAFG
                     VELPLRELFAAPTIAGLGAWLEALRADPPPVPAAPPLVATEPGPAELSFSQQRLWMLD
                     QIEPGGTAYVMSGALELAGVLDVPALERTLEALVNRHESLRTVFVSVEGKPQQVVSEP
                     RPRALPVADLGGEASAGERLGERLRAEAARGFDLARGPLFRAQLYRLAADRHVLLLTM
                     HHIISDGWSLGVLTRELGEFYRSFARGEAPGLPALRVQYRDYARWQRGWLQGEVLASQ
                     LARWRERLAGAPQVLELPTDRARPAAESFRGALHSFMVPLELTDALRALARNEGSTLF
                     MLLLSAYALLLARYSGQKDLLIGTAVANRHPAEVEPLVGFFVNTLVLRADLSGEPSVR
                     EFLGRMREMCLDALAHQDLPFERLVEELRPERDMSRNPVFQVMFTLQNAPMGPLELPG
                     LTLNPVDVGVAAAQFDLSLLAEETPEGLTASFTYATDLFDATTIARMGAHWRALLEAM
                     VAEPGRRVFELMLLSERERRQLLVEWNDTKTDYPREKCVHELFGAQAACTPDAVAVAC
                     GNQRLTYRELDARSSQLARFLQERGVVPDTLVPICVERSLEMVIGLLGILKAGGAYVP
                     LDPIYPADRLGFMLEDTEASLLLTQAHLLPKLPGRGATTICLDTEWHELANLPRTSPA
                     SRTNSGHLAYVIYTSGSTGRPKGVCITHRGLVNYLSFATGAYEVAQGTGAPVHSSIAF
                     DLTVTSLFAPLL"
BASE COUNT          486 a         1058 c          980 g          427 t
ORIGIN      
        1 agcagcgggg cgaaaagact cgtgacggtc aagtcgaagg cgatggacga gtgcactggc
       61 gccccggtgc cctgcgcaac ctcataggca cccgtggcaa agctcaggta gttgacgagg
      121 ccccggtgcg tgatgcatac ccctttcggc ctgcccgtgg atcccgacgt gtagatgaca
      181 taggcgaggt gccccgagtt cgtccggctc gccggtgagg tccgtggcaa gttcgcgagc
      241 tcgtgccatt cggtatcgag gcaaatcgtc gtggcgccac gcccggggag cttgggtagc
      301 aggtgcgcct gggtgagcag caacgatgcc tcggtgtcct cgagcatgaa gcccagtcgg
      361 tccgcggggt agatcgggtc cagcggcacg taggcgccac ctgccttgag gattccgagc
      421 aggccgatta ccatttccag ggatcgctcg acgcaaatcg ggaccagcgt gtcgggcacc
      481 acgccgcgct cctgaaggaa gcgtgcgagc tgacttgaac gtgcatccag ctcgcggtag
      541 gtaaggcgct ggttcccgca tgccactgcc acggcatcgg gcgtgcacgc ggcctgtgcc
      601 ccgaacagct cgtgaacgca cttctcgcgc ggatagtccg tcttcgtgtc gttccactcg
      661 accagcagtt ggcgacgctc ccgctcgctc aacagcatga gctcgaacac gcggcgcccg
      721 ggttcagcga ccatcgcctc cagtagcgcg cgccagtgcg cacccatgcg cgcgatcgtc
      781 gtggcgtcga agaggtcggt cgcgtaggta aagctggcgg tcagaccttc cggcgtttcc
      841 tccgccagca gcgagaggtc gaactgggcg gccgccacac ccacgtcgac gggattcagc
      901 gtaagtcccg gcaactccag ggggcccatc ggcgcgttct gcaacgtgaa catcacctgg
      961 aacacggggt tgcgactcat gtcgcgctcg gggcgcaatt cctcgaccag gcgctcgaag
     1021 ggcaggtcct ggtgcgcaag ggcgtccagg cacatctcgc gcatgcgacc gagaaactcc
     1081 cgcaccgagg gctcgcccga gagatccgcg cgcagcacca gcgtgttcac gaagaagccg
     1141 accaggggct cgacctctgc ggggtgtcgg ttggccaccg ccgtgccgat caggaggtcc
     1201 ttctgcccgc tgtagcgcgc gagcagcagc gcgtaagcgg acagcagcag catgaagagg
     1261 gtggacccct cgttccgcgc gagtgcgcgc agtgcgtcag tcaattccag gggcaccatg
     1321 aacgaatgga gcgctccgcg gaagctttcc gcggccgggc gcgcccgatc cgtgggcaac
     1381 tccagtacct ggggcgcacc cgccagacgc tcacgccagc gagccagctg cgacgcgagc
     1441 acctctccct gcagccaacc gcgctgccag cgcgcgtagt cacgatactg gacacgcagc
     1501 gccggcagcc cgggcgcctc gccacgcgca aagctgcggt aaaactcgcc cagctcgcgc
     1561 gtgagcaccc ccagcgacca gccgtccgag atgatgtggt gcatcgtcag caggagcacg
     1621 tggcggtccg ccgccagccg gtaaagctgt gcgcggaaca gcggcccgcg cgccagatcg
     1681 aacccgcgtg cggcctccgc gcgcaggcgt tccccgagcc gctcgccggc gctcgcctcc
     1741 ccgcccagat ccgcgaccgg gagtgccctt ggccgcggct ccgagacgac ctgctgcggc
     1801 ttgccctcca cgctgacaaa caccgtgcgc agcgactcgt ggcgattgac cagcgcctcc
     1861 agcgtgcgct ccagcgctgg cacgtcgagc acgccggcca gctcgagcgc tcccgacatg
     1921 acgtaggcgg tcccacccgg ctcgatctga tcgagcatcc aaaggcgctg ctgcgaaaag
     1981 gagagctcgg cgggacccgg ttccgtagcg acgagcggcg gcgcggcggg cacgggcggt
     2041 ggatccgcgc gaagcgcctc gagccacgca cccagccccg caatcgtcgg ggcggcaaac
     2101 agctcacgca acggcagttc caccccgaac gcctggcgca tccgcgagac gacctgcatg
     2161 gcaagcaggg aatgtccccc gaggtcgaag aagtcgtcct ccacgccgag gcggtcgagc
     2221 ttgagcacct cggcccagat gccggacagg atctcctcgt tccgcgtgcg cggcgcaacg
     2281 tatgccgcct tcaagcgggc ccgtcccggc gccggcagcg ccctgcgatc gaccttgccg
     2341 ttgggcgtga gcggcaactc gtcgagcacc acgatcgccg agggcaccat gtattccggc
     2401 agcgcctcgg cgaggtaatc gcgcagccgc ggcgtcaggt cgcgtaccag cttcgccttc
     2461 agcgggttgt tcgcatacgc cgcccagggc ttgcgcaccc ggttcgccgg ctgtgcccag
     2521 aagacctcgc ccggtctcac cgtgccgcgc ttgcggtaga ggacgtccat gtccgccttc
     2581 gagccgccac cggcgtagcc gagctccacc ccatagccgt gctcctcggc gatcgagcat
     2641 agcgcctcgg gctccagtgc ctcgccgggc tgcgcggcga tcgcctgccg gaagccttcc
     2701 agcgtctgcg gcccgtcttc cccgccggcc agccagtcga gcgctttcgc cgccgcttcc
     2761 aggcgcgcgt tggggacgcc acggacaccg agcaccggcg ggcccgaggc cagacgttcc
     2821 tgcaggtccg ccaggcgcaa gccgtcctcg cgccagtcca gccacgacgg cggcggcgcc
     2881 gctcctcccc tggactcgac atgcacgaac acgtcgtagc gaaagcgcgt gagctcgttc
     2941 tggtggcggc c
//
LOCUS       DAORUQ010000292         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_47891_length_2932_cov_4.602016, whole genome shotgun sequence.
ACCESSION   DAORUQ010000292 DAORUQ010000000
VERSION     DAORUQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47891_length_2932_cov_4.602016"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLW13_15640"
     CDS             <1..527
                     /locus_tag="PLW13_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71862.1"
                     /translation="AIGLALLGGEHLGSAEVEHAAAFLARHRDADPAALARTLIAAEA
                     EAQNAADRRIAPGFGTLFGGIDPQAGALAALLGAAPGAWPVLAWGGRFAACLREAGCG
                     WLAPGVAAAACIDLGFAARAAGALYQIASLPGLLAHGLEMSRQGLQAMPFVPDENYEF
                     LDPRDGVTVRDPAI"
     gene            524..1345
                     /locus_tag="PLW13_15645"
     CDS             524..1345
                     /locus_tag="PLW13_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71863.1"
                     /translation="MNAPTIWETRRHRIVTRKGGWRIGEGVRVGAYSLLDDMVGEHTF
                     FELLFLEVTDRLPSPELARWIEACFLCLSFPDPRIWCNQIGAFAGTMRCAPGAAVSAG
                     TLASDSGMYGPGAARSACEFIEEVTREVRTGESIESIVARRTTRSGRLRAPGYSRPIA
                     SGDDRVGALCRFAAELGIPDGEHLGVAWRLDAHLRTHSADSLNMLGYVAAFVLDNGIS
                     TEESYRLFTLIVNGGIHACYSEAADEPAGTFLPLRVEDVEYTGVPSRPLPRGNTA"
     gene            1342..2298
                     /locus_tag="PLW13_15650"
     CDS             1342..2298
                     /locus_tag="PLW13_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71864.1"
                     /translation="MRRRTFARRSLGVLCAAALAAILPAVPALAADSAAPRVAVVYPQ
                     VRDPYRAVFRAIVDGINAETAQGAEVFEIAPEETIEAVTARLAERQLDAVVLLGKRGL
                     DLSKLIESPERRVVGAVFAKPGDIAAGVSATSLAPAPRMLFSKLRELAPAVKRIHVIY
                     GADDNDWLIELARSDAANLGYELVPQRKESIREGASAYRELLKQVESPQDAIWLLQAS
                     PFLDENSVLQMVLRAAWDRNLVVFSSNPSHVPKGALFALFPDNNAMGRHLAQQLRKPA
                     SPSARLLPLEQLDTAINIRTADHLGLGLSAGDSRFDMVFPNR"
     gene            2322..>2932
                     /locus_tag="PLW13_15655"
     CDS             2322..>2932
                     /locus_tag="PLW13_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71865.1"
                     /translation="MPRTAKLIRSMSLQQLIGISFTACVLLLAVASSLVISKQSGDTV
                     QRRLQDEGMRLVESLAQQSTLALLYEDAGSAAEVVKSFLNFPDVYGIELVYADGKRLY
                     ASDSLREARARAFATPTHPALIAQDSSDWTFAAPVFSGKADSESPFAASAGDTPEMIG
                     SVRLVMSRQTLSSMQAEIVRASLLVAVVLSALLLMVLLGITSRL"
BASE COUNT          437 a         1039 c         1002 g          454 t
ORIGIN      
        1 gcgcgatcgg cctcgcgttg ctcggcggcg agcacctcgg cagcgccgag gtcgagcacg
       61 ccgcggcgtt cctcgcgcgc caccgcgacg cggatcccgc ggcgctggcc agaacgctga
      121 ttgccgccga agccgaggcg cagaacgcag cggaccgccg cattgcgccc ggtttcggca
      181 ccctgttcgg cggcatcgac ccgcaggccg gcgcgctagc cgcgctgctc ggtgcggctc
      241 ccggcgcgtg gccggtgctg gcctggggcg ggcgcttcgc ggcctgcctg cgcgaagcgg
      301 gttgcggctg gctcgcgccc ggcgtggccg cggccgcgtg catcgacctc ggtttcgctg
      361 cgcgcgccgc gggcgcgctg taccagatcg cgtcgctgcc cgggctgctg gcgcatggac
      421 tggaaatgag ccggcagggc ctgcaggcca tgccattcgt tccggacgag aattacgagt
      481 tcctggaccc gcgcgacggc gtgacggtgc gagatcctgc catatgaatg cgccgacgat
      541 ctgggagaca cgacgccatc gcatcgtgac gcgcaagggc ggttggcgca tcggcgaggg
      601 tgtgcgcgtc ggcgcctatt cgctgctcga cgacatggtc ggcgagcata cttttttcga
      661 actgctgttt ctcgaggtga ccgatcgctt gccgtcgcca gagctggcgc gatggatcga
      721 ggcctgcttc ctgtgcctgt cattccccga ccctcgcatc tggtgcaacc agatcggcgc
      781 cttcgccggc actatgcgct gcgcgccggg cgcggccgtg agcgcgggca cgctcgccag
      841 cgactcgggg atgtacggcc cgggcgctgc acgcagcgcc tgcgagttca tcgaagaggt
      901 gacccgggag gtgcgcaccg gcgagagcat cgagtcgatc gtcgcacggc gcaccacgcg
      961 cagcggccgc ctgcgcgcgc cgggctattc gcgtcccatc gcgagcggcg acgatcgcgt
     1021 cggcgcactg tgccgtttcg ccgccgaact cgggattccc gacggagaac atctgggcgt
     1081 ggcgtggcgc ctggacgccc acctgcgcac acacagcgcc gattcgctga acatgctggg
     1141 ctacgtcgca gccttcgtgc tcgacaacgg catcagcaca gaggagagct accgtttgtt
     1201 caccctgatc gtgaacggtg gcatccacgc ctgctacagc gaggccgccg acgagccggc
     1261 cggcactttc ctgccgctgc gcgtggagga cgtcgaatat accggcgtgc cgtcgcgtcc
     1321 gctgccgcgg ggaaacaccg catgaggcgc cgcacgtttg cccgccgctc gctcggcgtg
     1381 ctctgcgcgg cggcgctggc cgcgatcctg cccgccgtgc cggcactcgc cgccgacagc
     1441 gctgcgcccc gtgtcgcggt ggtctatccg caggtccgcg acccctaccg cgcggtgttc
     1501 cgcgccatcg tcgacggcat caacgccgag actgcgcagg gtgccgaggt gttcgagatc
     1561 gcgcccgagg agaccatcga ggcagtcacc gcacgcctcg ccgagcgcca actcgacgcc
     1621 gtggtgctgc tcggcaagcg cggcctcgac ctgagcaagc tcatcgagag tcccgagcgg
     1681 cgcgtcgtgg gcgcggtatt cgccaagccc ggcgacatcg ccgccggcgt gagcgcgacc
     1741 tcgctcgcgc ccgcaccacg gatgctgttc agcaagctgc gcgagctcgc ccccgccgtg
     1801 aagcgcatcc acgtgatcta cggcgccgac gacaacgact ggctgatcga gctggcgcgc
     1861 agcgacgcgg cgaacctcgg ctacgagctg gtgccgcagc gcaaggagag catccgcgag
     1921 ggcgcgagcg cctatcgcga gctgttgaag caggtcgagt caccgcagga cgcgatctgg
     1981 ctgctgcagg cctcgccctt cctcgacgag aactcggtgc tgcagatggt gctgcgcgcg
     2041 gcctgggacc gcaacctggt ggtgttctcc agtaacccct cgcacgtacc caagggcgcg
     2101 ctgttcgcgc tgtttcccga caacaacgcc atgggccggc acctcgcgca acagctgcgc
     2161 aagcccgcgt cgccctcggc aagactgctg ccgctcgagc agctggacac cgcgatcaac
     2221 atccgcacgg ccgaccacct cggcctcggg ttgagcgccg gtgactcccg tttcgacatg
     2281 gtattcccga accgctaggt tccggacgag agcacgcgcc catgccaaga acagcaaaac
     2341 tgatccgctc gatgagcctg cagcagctca tcggcatcag cttcaccgcc tgcgtgctgc
     2401 tgctcgcggt cgcctcgagc ctcgtgatat ccaagcaatc cggcgacacg gtgcagcgcc
     2461 gcctgcagga cgaaggcatg cgcctggtgg aatcgctggc gcaacagagc acgctcgccc
     2521 tgctgtacga ggacgcgggc tcggcagcgg aagtggtcaa gagcttcctg aacttccccg
     2581 acgtctacgg catcgagctg gtgtacgccg acggcaagcg gctctacgcc agcgattcgc
     2641 tgcgcgaggc acgcgcgcgg gcattcgcca ccccgacgca tcccgcgctg atcgcgcagg
     2701 acagcagcga ctggaccttc gccgcgccgg tgttctcggg aaaggccgac agcgaatcgc
     2761 cgttcgcggc cagtgcgggc gatacgccgg agatgatcgg cagcgtgcgc ctggtgatgt
     2821 cgcgccagac gctctcgagc atgcaggccg agatcgtgcg cgcgagcctg ctggtcgcgg
     2881 tggtgctctc ggcactgctt ctgatggtat tgctcggcat cacctcgcgc ct
//
LOCUS       DAORUQ010000293         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_47983_length_2929_cov_5.259569, whole genome shotgun sequence.
ACCESSION   DAORUQ010000293 DAORUQ010000000
VERSION     DAORUQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47983_length_2929_cov_5.259569"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLW13_15660"
     CDS             complement(<1..409)
                     /locus_tag="PLW13_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY71866.1"
                     /translation="MSDEVLLHEADGILTITINRPAAKNAVNLAVATGIAAAIDRLDA
                     SPALRVGILTGAGSAFCSGMDLKAFLTGEMPMLPGRGFGGLVEAPPQKPLIAAIEGYA
                     LAGGLELMLACDLAVAGENARLGIPEAKRGLVAA"
     gene            complement(516..1532)
                     /locus_tag="PLW13_15665"
     CDS             complement(516..1532)
                     /locus_tag="PLW13_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014860237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQY71867.1"
                     /translation="MVNLVRAAALTKYAEVARQCGLDPVSLLRKHGMSLRLIADPERR
                     IPIRPVLALLEESAQLADCPGFGLRMAESRQLADMGGISLLLTHQRTVGDAVRTLIEY
                     QDVLNPSISLSIEQAGKSVVVRSQLLTGGATHSVQGDELLIGVLYLMCKALLGAQWCA
                     RGVQFSHAAPADLGVHRRVFACPLEFGREFNGMIMNAADLALVNPGGDPAMARFARRF
                     LDSMLGAKNMQEHSIVREVRKAIYLMLPAGRATIEQIAAGMGVNLRTLQRHLEEGGVT
                     FSGLLDEVRGELVLRYLEDRELPLARIAELLGYRMPSSFTRWFNAQFGMAPVAWRRTL
                     TRTG"
     gene            1836..2516
                     /locus_tag="PLW13_15670"
     CDS             1836..2516
                     /locus_tag="PLW13_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQY71868.1"
                     /translation="MISSISLPLATRHLEPGESLSELLFGLIMTLTFTLGAGLMVQEG
                     PDAVSDLLVATIGCNIAWGIIDGVFYINGQVFERARIARLGRMIRAARNEDEAAIVVE
                     RELAEIIELTVDAGERGELYRRMARQVRAATSRTRPVTLVPSDFKGALASFWLVFVGS
                     IPAALPFLMIDDAWIALRVSNAILLGLLFFAGFRWARHTVLPPLRTGLLFLAAGVALV
                     AVAIALGG"
BASE COUNT          477 a         1020 c          964 g          468 t
ORIGIN      
        1 cggcagcgac caggccgcgc ttggcctcgg gaatgcccaa gcgcgcgttc tcgcccgcca
       61 ccgcgagatc gcaggccagc atcagttcca gaccaccggc cagcgcgtag ccctcgatcg
      121 cggcgatcag cggcttctgc ggcggcgctt cgaccaggcc gccgaatcca cgcccgggca
      181 gcatcggcat ctccccggtg aggaaggcct tcaggtccat gccggagcag aacgcgctcc
      241 cggcgccggt cagtatgccc acgcgcagcg ccgggctggc gtcgaggcgg tcgatcgccg
      301 ccgcgatgcc cgtggccacg gccaggttga ccgcgttctt cgccgccggg cggttgatgg
      361 tgatggtcag aatgccgtct gcctcgtgca gcaggacttc gtcggacatg ggggtgctcc
      421 cgtgatggct gggggtgtgg aacagcaggc gcgaatattc gtcgctggcg cggtcgctgt
      481 ccagagacgc gccgcgacga ccgctgcgcc acgggctagc ctgtgcgggt cagcgtcctg
      541 cgccaggcca ccggcgccat gccgaactgc gcgttgaacc agcgcgtgaa cgagctcggc
      601 atccggtaac cgagcagttc cgcaatgcgc gccagcggga gttcgcggtc ctcgagataa
      661 cgcagcacga gttcgccgcg cacctcgtcc agcagcccgg aaaaggtcac gccgccctcc
      721 tcgaggtggc gctgcagggt gcgcaggttc acgcccatgc ccgcggcgat ctgctcgatg
      781 gtggcacgcc ccgccggcag catcaggtag atggccttgc gcacctcgcg cacgatcgaa
      841 tgctcctgca tattcttcgc gccgagcatc gagtcgagga agcgccgcgc gaaacgcgcc
      901 atagccggat cgccccccgg attgaccagg gcgaggtcgg ccgcgttcat gatcatcccg
      961 ttgaactcgc gaccgaactc cagggggcag gcgaacacgc gccggtgaac gcccagatcc
     1021 gctggcgccg catgagagaa ctgcacaccg cgcgcgcacc actgcgcgcc gagcaacgcc
     1081 ttgcacatga ggtacagcac gccgatcagg agctcatcgc cctgcacgga gtgcgtggcg
     1141 ccgccagtca gcaactgcga gcgcaccacc acggacttgc ccgcctgctc gatggacagt
     1201 gagatcgagg ggttgagcac atcctggtat tcgatcaacg tccttacggc gtcgccgacc
     1261 gtgcgctggt gcgtcagcag caagctgatg ccccccatgt ccgcgagctg acgcgactcc
     1321 gccatgcgca gcccgaagcc cgggcagtcg gccagctgcg ccgactcctc cagaagcgcc
     1381 agcaccggcc ggatcgggat gcgccgctcg ggatcagcga tcagcctgag gctcatgccg
     1441 tgcttgcgca gcaggctgac ggggtcgagt ccgcattgtc tggcgacctc ggcgtacttc
     1501 gtgagcgcgg cggcgcggac caggttgacc atgtacgcga agctccggaa cagggaatac
     1561 cggcgagcgt gcaaaagccg cccgcaactg cagcggcaca cactagcgca tgcgtcatga
     1621 aatgcgaaga ttctgccgcg cctcggcggc cgccgttccc ggtgctgcgc tgcgcgacag
     1681 ttgctgctgc cggcgcgcga tatgaccgga gcgccatcgg ggcgcgatgt tcgcagctct
     1741 gcgggccgcg ttgcgcatcc gcgactgcgc atcaacctat ctatactcgt tcgcatcaat
     1801 ggtatcgctg atgtccacgc gcggagctac ctctcatgat cagcagcatt tccctgccac
     1861 tggccaccag gcatctggaa cccggagaga gcctgtccga actgctgttc ggactgatca
     1921 tgacactcac cttcacgctc ggtgccggcc tgatggtcca ggaaggcccg gacgcggtca
     1981 gcgatctgct cgtcgcgacc atcggctgca atatcgcctg gggcatcatc gacggcgtgt
     2041 tctacatcaa cggacaggtc ttcgagcgcg cgcgcatcgc gcgcctgggc aggatgatcc
     2101 gggctgcccg caacgaggac gaagccgcca ttgtcgtgga acgcgaactc gcggagataa
     2161 tcgaactcac ggtggatgcg ggcgagcgcg gcgagctcta ccgacgcatg gcccgccagg
     2221 tccgggcagc cacctcccga acgcgaccgg tgacgctggt gccctcggac ttcaagggcg
     2281 cgctagcgag cttctggctg gtgttcgtcg gcagcattcc ggccgcgctg ccatttctga
     2341 tgatcgacga cgcgtggatc gccctgcgcg tgtccaacgc gatcctgctc ggcctgctct
     2401 tcttcgccgg attccgctgg gcccggcaca cggtcctgcc gccgctgcgc acgggcctgc
     2461 tcttcctggc tgcgggcgtc gcgctggttg ccgtggcgat cgcgctgggc ggctgatcag
     2521 ccgccgtctc ctccttgaac gccagttccg tggccggcgc gtgaccgccc ctgcggtcac
     2581 ctgatgaaaa aatgcgtctt gcgccacgtt caattcggcg agtgcgtgag ctagagtgca
     2641 gcggcgttgc cgtcgcgccg cgccccccag gcgcggccac ctgtcgcaag ttccgggagt
     2701 atcgaatgag tcctaccagc aaagtgcatg catccgtccc ggggacgcta atcgcgagca
     2761 cggatgccgc gaagaagcag ccgagcgggg ccatcgtgat gatcggctcc tgcacgccgg
     2821 cgtgagcatc tccggcccaa tccccgcgca gtgcgctcgc accggccacg ccgtgcgcct
     2881 gcgcgagccg atcgaggcca tgaagctgcc agccggttac agcctgtcg
//
LOCUS       DAORUQ010000294         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_48786_length_2899_cov_5.641702, whole genome shotgun sequence.
ACCESSION   DAORUQ010000294 DAORUQ010000000
VERSION     DAORUQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48786_length_2899_cov_5.641702"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PLW13_15675"
     CDS             complement(<1..1361)
                     /locus_tag="PLW13_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQY71869.1"
                     /translation="MEHLIKGAPLSEERLGALTLGGYLREVCGKYAANEAMVFHPPSG
                     PVVRLTYAQVWDEAFAIARALLARGVSKETRVGVLATNRPEWVSAMFGISLAGGTAVL
                     LSSFARGAELEYLLRVADVSLLIFERSVLNRDFAAEIGEFCPELASARGDVHAPRLPF
                     LRRAVCIGEMDGLGGAIESWQEFVHGERKAPAAVVEAIAAEVAPSDRGLVFFSSGSTS
                     KPKGVVHTHRAATIQCWRWPRMYALEPGVRTWSANGFFWSGNFTQAIGATFTVGGCLV
                     LQRYFDPGEALRLMQAERVTLPVAWPHQWVKLAEDPAFHEVDLGSLRYVGETSPLRNH
                     PTVRSNWQEPMSSYGNTETLTFSANHPSGTDPAIVAGNYGYALPGNTFKIVDPLSGEA
                     LARGETGEIAVKGPTLMVSYLRMAPDEVFDEEGFFHTGDGGFFDAEGRLHWHGRLNDI
                     IKTG"
     gene            complement(1362..1994)
                     /locus_tag="PLW13_15680"
     CDS             complement(1362..1994)
                     /locus_tag="PLW13_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY71870.1"
                     /translation="MSNETTPRLKPLLPPDWDDTIHDAASAFPHARDFVLNNWNTDDA
                     RGVNGVGMMLNHPALAKAFLTFNNHVASASTVSRRIRELLILRIGWLRRSEYEFYQHI
                     VLGRRAGLSDEEIVRIESGPDAPGWDPVDAELLRAVDELHADACIGDATWARLSAHFD
                     AKQLMDIVFAVGCYDVLAMAFKTFGVPFEPGVEPLAPDVRARMFATRTKQ"
     gene            complement(2080..>2899)
                     /locus_tag="PLW13_15685"
     CDS             complement(2080..>2899)
                     /locus_tag="PLW13_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY71871.1"
                     /translation="KEIAAPGNWKVAQEAFFESYHVPTTHPQLETAGADFIFGDQSGP
                     AVDFSHHYHQYETFAHGHGRFFGGKKTPMAGHVTLPGDPVEMMAARLNLLVEGMDAMV
                     LAEDVELVRSLKGKPIPEGSSLGGEYMKLLYSSAAAQNRPMPKPAPEVLDMWGGEIFV
                     FPNLMILPQAGNAMIYRVRPHRTDPDRCIFEILSTRTYPAALAPPRATVEKVVDPSDP
                     GQLRLIPRQDLGNIPRMQRGLHSRGCKQIWLARYYEQIILHMHQELDRYLLAGA"
BASE COUNT          468 a          987 c          970 g          474 t
ORIGIN      
        1 ccggtcttga tgatgtcgtt caggcgcccg tgccagtgca gccgcccctc ggcatcgaag
       61 aagccaccgt cgccggtatg gaagaagccc tcctcgtcga acacctcgtc gggcgccatg
      121 cgcaggtagc tcaccatcag cgtggggccc ttgacggcga tctcgccggt ctctccgcgc
      181 gccagcgcct cgccgctcag cggatcgacg atcttgaagg tgttgccggg cagcgcatag
      241 ccgtagttgc ccgcgacgat cgccgggtcg gtgccgctcg ggtggttggc gctgaaggtc
      301 agcgtctcgg tgttgccgta ggacgacatc ggctcctgcc agttcgagcg caccgtggga
      361 tggttgcgca gcggcgaggt ctcgccgacg tagcgcagcg aacccaggtc cacctcgtgg
      421 aacgccgggt cctcggccag cttgacccac tggtgcggcc aggccaccgg cagcgtgacg
      481 cgctcggctt gcatcagccg cagcgcttcg cccggatcga aatagcgctg cagcaccagg
      541 caaccgccaa ccgtgaacgt ggcgccgatg gcctgcgtga aattacccga ccagaagaag
      601 ccgttggccg accaggtgcg cacgccgggc tcgagcgcgt acatgcgcgg ccagcgccag
      661 cactggatcg tggcggcgcg atgcgtgtgc accacgccct tgggcttgga ggtcgagccc
      721 gaggagaaga acaccaggcc gcggtccgag ggcgcgacct ccgcggcgat ggcctcgacc
      781 acggcggccg gcgccttgcg ctcgccgtgg acgaattcct gccagctctc gatggccccg
      841 ccgagcccat ccatctcgcc gatgcatacc gcgcggcgca ggaagggcag gcgtggcgca
      901 tggacatcac cgcgcgcgga cgcgagctcg gggcagaatt cgccgatctc ggcggcaaaa
      961 tcgcgattca gcaccgagcg ctcgaagatc agcagcgaga cgtcggcgac acgcagcagg
     1021 tattcgagct ccgcgcccct ggcgaaggag ctcagcagca cggcggtgcc gcccgcaagg
     1081 ctgatgccga acatcgcgct cacccactcc gggcgattcg tcgccagcac gccgacgcgc
     1141 gtctccttgc tcacgccgcg cgcgaggagt gcgcgcgcga tcgcgaaggc ctcgtcccag
     1201 acctgcgcgt atgtgaggcg caccacgggc cccgacggcg gatggaacac catcgcctcg
     1261 ttggcggcgt atttcccgca gacctcgcgc aggtagccgc cgagagtcag cgctcccagc
     1321 cgctcctccg agagcggagc tcccttgatc aagtgttcca tctactgctt cgtcctggtt
     1381 gcgaacatac gcgccctcac gtccggcgcc aacggctcca cgcccggctc gaagggcaca
     1441 ccgaaagtct tgaacgccat ggccagcaca tcgtagcagc ctaccgcgaa cacgatgtcc
     1501 atgagctgct tcgcgtcgaa atgcgcgctg agccgcgccc aggtcgcatc accgatgcag
     1561 gcatccgcgt gcaactcgtc cacggcgcgc agcagctcgg cgtcgaccgg gtcccagccg
     1621 ggcgcgtcgg gacccgactc gatgcgcacg atctcctcgt cgctgaggcc ggcgcggcga
     1681 cccagcacga tgtgctggta gaactcgtat tccgagcgtc gcaaccagcc gatgcgcagg
     1741 atcagcagct cgcggatgcg cctggacacg gtgctcgcgg aggcgacgtg gttgttgaag
     1801 gtcaggaatg ccttcgccag cgcgggatga ttcagcatca tgcccacccc gttcacgccg
     1861 cgggcatcgt cggtgttcca gttgttcagc acgaagtcgc gcgcgtgcgg aaaggcgctg
     1921 gccgcgtcgt ggatcgtgtc gtcccagtcg ggcggcagca atggcttgag ccgcggcgtt
     1981 gtttcgttcg acatgcgtgg tttccggttg gatcactgtt tcggcggcgc gtgacaggca
     2041 ccggcgctcg agagcatggg ccgcgttagc acattcccgt cacgcgcccg caagcaggta
     2101 gcggtcgagc tcctggtgca tgtgcaggat gatctgctcg tagtagcgcg cgagccagat
     2161 ctgcttgcag ccgcgtgagt gcagcccgcg ttgcatgcgc gggatgttgc ccagatcctg
     2221 gcgcgggatc agccgcaact gcccgggatc ggacggatcg acgaccttct ccaccgtcgc
     2281 gcgcggcggc gcgagcgcgg cgggataggt cctggtggac aggatctcga agatgcagcg
     2341 atcgggatcg gtacggtgcg gccgcacgcg atagatcatc gcgttgcccg cctgcggcag
     2401 gatcatcagg ttcgggaata cgaaaatctc gccaccccac atgtccagca cttcaggcgc
     2461 gggcttgggc atcgggcggt tctgcgccgc ggcgctcgag tagagcagtt tcatgtactc
     2521 gccgccgagg ctcgagccct ccgggatcgg cttgcccttc agcgagcgca ccagctcgac
     2581 gtcctcggcg agcaccatcg cgtccatgcc ctcgaccagc aggttcaggc gcgccgccat
     2641 catttccacc ggatcgccgg gcagcgtgac gtgacctgcc atcggcgtct tcttgccgcc
     2701 gaagaagcgc ccgtgtccgt gcgcgaaggt ctcgtactgg tggtagtggt ggctgaagtc
     2761 caccgcgggc ccgctctggt cgccgaaaat gaaatccgcg cccgcagtct cgagttgcgg
     2821 gtgcgtggtc ggcacgtggt aggactcgaa gaatgcctcc tgggccactt tccagttgcc
     2881 gggcgccgcg atctccttc
//
LOCUS       DAORUQ010000295         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_48847_length_2897_cov_4.577762, whole genome shotgun sequence.
ACCESSION   DAORUQ010000295 DAORUQ010000000
VERSION     DAORUQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48847_length_2897_cov_4.577762"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLW13_15690"
     CDS             <1..608
                     /locus_tag="PLW13_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HQY71872.1"
                     /translation="APVNIFDEAAFQKVIEEHGADSERNLGAKADTIAHATRRAIHER
                     LEQDPAFYEKFSRLIQQAIDDYRARRLSDLEYLQRVTEIKDAVVSRKGDDLPPALNGD
                     ADAAAVYGLLRPFVTNHVGDAEQAQTTAAEAAMAVWDIFRRNRKVGYWDDLDAQRRTM
                     NEIDDYLYDEVKGARGIALSTDEMDEIIEKSMQLARHRMAG"
     gene            614..1315
                     /locus_tag="PLW13_15695"
     CDS             614..1315
                     /locus_tag="PLW13_15695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQY71873.1"
                     /translation="MVGSLTYGRDTIRYEVRFLASRQTLAIEVHPDSRVLVRAPLGCP
                     EAVIAQRVQKRAGWISRQLAEFERYRPRTPTRQYVNGESHLYLGRQCRLKLVTGDSTS
                     VKLTRGRLLVSLPDNPDPERVKALLHRWYLDRARVVFADVLDASLRRFRGTDRPRLIV
                     RTMQSRWGSLSRAGTMTLNVNLVRAPRSCIEYVVIHELCHTRHRDHDARFFRLLGQVM
                     PDWEERKRKLEAALL"
     gene            1472..2359
                     /locus_tag="PLW13_15700"
     CDS             1472..2359
                     /locus_tag="PLW13_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71874.1"
                     /translation="MKTQHLAGRNAVVTGSSMGIGRAVALELARHGANVVLNSSSSAA
                     ALAQTVAEITATGGRAIGRLGSVAEYDFAGSLVADCVGEFGAIDILVNVAGIAEPAGS
                     SILDIDPADWRRQIDVHLHGTFNTCRHAAPLMAAQKRGAIVNTASHALLGVFGGTGYA
                     AGKGGTLSLSWAIARELAGHGVRCNVVCPGAESRLSTGPEYEALIAGLNARGILDDRM
                     RDAALNPVPARFLAPAYAFLASDLAQDINGKLFSVAGGYVGLFSGLQEQFLAYKDHRG
                     GETWTLEELAGQIARAGLA"
     gene            2449..>2897
                     /locus_tag="PLW13_15705"
     CDS             2449..>2897
                     /locus_tag="PLW13_15705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQY71875.1"
                     /translation="MFRRSLALLGLGLACALPAHAATVSGTVSSEQGVPVALAQVSLS
                     NLELGRTTTVYADAEGRYRLDGLLPGHYRFRARRIGFGTVEESGISLAHDAVLERAVT
                     LKPLPKSLWIHELPASDWYAGAQFSSPALGAQFAIQCLMCHQQGSSTT"
BASE COUNT          474 a         1000 c          973 g          450 t
ORIGIN      
        1 acgcgccagt gaacattttc gatgaggccg cgttccagaa agtgatcgag gaacacggcg
       61 cggactccga gaggaacctt ggcgctaagg ccgacacgat tgcccatgcg acccggcgcg
      121 ccatccacga acggctggag caggacccgg ccttttacga gaagttctcc cggctgatcc
      181 agcaggccat cgacgactac cgcgccaggc gtctgtccga tctggagtac cttcagcgcg
      241 tcaccgagat caaggatgcc gtcgtctccc gcaagggcga cgacctgcct ccggcgctga
      301 atggtgacgc cgacgcggcc gccgtctatg gcctgctgcg gccctttgtg accaatcacg
      361 tcggcgatgc cgagcaggcg cagacgaccg ccgccgaggc cgccatggcg gtatgggaca
      421 tattcaggcg caatcgcaag gtgggttact gggacgacct cgatgcccag cgccggacga
      481 tgaacgagat cgacgattac ctctacgacg aggtgaaggg cgcgcggggg atcgcgctgt
      541 ccaccgacga gatggacgag atcatcgaga aatccatgca gcttgcccgc caccggatgg
      601 cggggtgaaa accatggttg gcagcctcac ctacggccgc gacaccatcc gctacgaggt
      661 gcgcttcctg gcatcacgac agacgctggc catcgaggtg caccccgaca gccgcgtgct
      721 ggtgcgcgca ccgctgggct gcccggaagc cgtgatcgcg cagcgggtac agaagcgcgc
      781 gggctggatc agccggcaac tggcggagtt cgagcgttat cgcccacgca ccccgacgcg
      841 gcagtatgtc aacggcgaat cgcatctgta cctcggccgg caatgccggc tgaagctggt
      901 gacgggcgat tcgaccagtg tgaaactcac gcgcggccga ctgctcgtca gcctgcccga
      961 caaccctgac ccggaacgcg tcaaggcgct gctgcaccgc tggtatctcg accgcgcgcg
     1021 ggttgtcttt gctgacgtgc tggacgccag tctgcgccgc ttcaggggca ccgatcgccc
     1081 gcgcctgatc gtgcgtacca tgcaatcccg ctggggcagc ctgtcgcgcg ccggcaccat
     1141 gaccttgaac gtcaacctgg tgcgcgctcc ccgctcttgc atcgagtacg tggtgattca
     1201 cgagctatgc catacccggc accgcgacca cgatgcgcga tttttcaggt tgctcgggca
     1261 ggtgatgccc gattgggagg agcgaaagcg aaagctggag gccgcgttgc tgtgagttgc
     1321 gtgccggcgg cagcctcagc cgctgtgcgc tccccggttc ctgaaaagag ttatggtgaa
     1381 ttattttttt tgacactgcg aggcggcgcc cggtagagtc cggcgcgcgc cagcgcagct
     1441 ctcccaccgc acacagggac gcctccgacc cgtgaaaacg caacatctcg ccggccgcaa
     1501 cgccgtcgtc accgggtcca gcatgggcat cgggcgcgcc gtggcgctcg agctcgcgcg
     1561 ccatggcgcc aacgtcgtgc tcaacagttc ctcgagcgcg gcggcgctcg cgcaaacggt
     1621 ggctgaaatc acggccacgg gcgggcgggc catcggccgc ctcgggtccg tcgcggaata
     1681 tgactttgcc ggcagcctgg tcgcggactg cgtgggcgag ttcggtgcca tcgacatcct
     1741 ggtcaacgtc gcgggcatcg cggaaccggc cggcagctcg atcctggaca tcgatcccgc
     1801 cgactggcgc cgccagatcg atgtgcacct gcacggcacc ttcaacacct gccgccacgc
     1861 cgcgccgctg atggcggcgc agaagcgcgg cgcgatcgtc aacaccgcct cgcacgcgct
     1921 gctcggcgtc ttcggcggca ccggctacgc cgccggcaag ggcggcacgc tcagcctcag
     1981 ctgggccatc gcgcgcgagc tcgccgggca cggggtgcgc tgcaacgtgg tctgcccggg
     2041 cgcggagagc cggctctcga cggggccgga gtacgaggcg ctgatcgcgg ggctgaacgc
     2101 gcgcggcatc ctcgacgacc ggatgcgcga cgccgcgctg aacccggtgc cggcgaggtt
     2161 cctggcaccg gcctatgcct tcctcgccag cgatctcgcg caggacatca acggcaagct
     2221 cttttccgtg gccggcggct acgtcgggct gttcagcggc ctgcaggagc agttcctggc
     2281 ctacaaggac caccgcggcg gcgagacgtg gacgctggag gaactcgccg ggcagattgc
     2341 ccgcgcgggc ctagcctgat ccaggcaagc gcgcgccgcc cgggcggtcg cattcacatc
     2401 gtttgcggcg ctgccggagt gacagcgcca gaccggggag ggaaacacat gtttcgccgt
     2461 tcgcttgcgc tgctcggcct gggcctggcc tgtgccctgc cggcccacgc cgccaccgtc
     2521 agcggcaccg tcagcagcga gcagggcgtc ccggtggcac tggcgcaggt gtcgctgagc
     2581 aacctcgagc tcgggcgcac cacgaccgtc tatgccgatg ccgagggccg ctaccgcctc
     2641 gacggtctgc tgcccggcca ctatcgattc cgcgcgcggc gcatcggatt tggcacggtg
     2701 gaggagagcg gcatctcgct ggcgcacgac gccgtgctcg agcgtgccgt gacgctgaag
     2761 ccgctgccca aatcgctgtg gatccacgag ctgcccgcca gcgactggta tgccggcgcg
     2821 cagttctcct cgcccgcgct cggcgcgcag ttcgccatcc agtgcctgat gtgccaccag
     2881 cagggttcca gcaccac
//
LOCUS       DAORUQ010000296         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_49567_length_2871_cov_7.486506, whole genome shotgun sequence.
ACCESSION   DAORUQ010000296 DAORUQ010000000
VERSION     DAORUQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49567_length_2871_cov_7.486506"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..422
                     /locus_tag="PLW13_15710"
                     /pseudo
     CDS             1..422
                     /locus_tag="PLW13_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(655..948)
                     /locus_tag="PLW13_15715"
     CDS             complement(655..948)
                     /locus_tag="PLW13_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY71876.1"
                     /translation="MWQIAALAEKHDLVVCSDEIHNGLVLDPACRHRVLASLSPEVAA
                     RSITLMAPSKTFNIPGLGAAFAIISDPAVRRRFRAAMRRIGNSTAIPEVSPHP"
     gene            965..1195
                     /locus_tag="PLW13_15720"
     CDS             965..1195
                     /locus_tag="PLW13_15720"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71877.1"
                     /translation="MPPGLYGRHRLEVSDAALALIAEAGFDPVFGARPLKRAIQERIE
                     NPLSRLILDGTLGAKDRVKVEATKGQISFVKA"
     gene            1388..2788
                     /locus_tag="PLW13_15725"
     CDS             1388..2788
                     /locus_tag="PLW13_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY71878.1"
                     /translation="MPTQAAKPCQEASVYQRRRPERTLLYHLVQAHLATWLALQDDGE
                     GRSASAPALVEREFRRYLDCGILAHGFARARCADCGHDFIVAYSCKGRGVCPSCTTRR
                     MVETAAHLVDHVFPALPVRQWVLSLPKRLRYHLDDAKLQSAVLHSFLRAIEQGLRQSL
                     PEGDGTARIGAVVFIHRFGALMNAHLHFHVIAIDGVFFGEETASLRFEETHLSAQALA
                     RLQSTLRKRIVSLFVRRGLLDSAEGEALRRCAHGGGFSLDAGVRIEGDDRQGRERLLR
                     YCARPAFAQERLRQIDAEHLVYESPKPGPGGRVSQILTPLELLDRLAALIPPPRRHRH
                     RYYGVLAPNAPLRSAVTALAAAPKPPSVEADAVAEESAIRRAARYAWALLLARIYEVF
                     PLTCPKCGGAMRIIAFIDEGEAIRKILQHLGEPIEPPKLAPARGPPLWEAAGQGHADP
                     LAQSIPEFEFDQRIAW"
BASE COUNT          456 a          989 c          971 g          455 t
ORIGIN      
        1 ccaagccggg accgggcggg cgcgtcagcc agatcctgac gcccctcgaa ctgctcgatc
       61 gcctcgccgc gctgatcccg ccgccccggc gccaccggca tcgttactac ggcgtgctgg
      121 cgccgaatgc cccccttcgg tcggcggtca cggcactggc agcggcgccg aagccaccct
      181 cggtcgaggc cgatgccgtg gcggaggaaa gtgcgatccg tcgggcggcg cgctatgcct
      241 gggcgctctt gctggcgcgc atctacgagg tgttcccgct cacctgccca aagtgcggcg
      301 gcgcgatgcg gatcatcgcc ttcatcgacg agggcgaggc gatccgaaag atcctccagc
      361 acctgggcga gccgatcgaa ccgcccaagt tggccccggc gcgcggcccg ccgctgtggg
      421 aggcggcggg acagggccac gccgatccgc tggcgcagtc aatccccgag ttcgagttcg
      481 atcagcggat cgcgtggtag cggacggcgg cgaacgccgc ccgacgggaa cgctcttgcc
      541 cgaggcccgc aatacgcccg acagagggcg tattgcgggc ctcgggcaag gttgggcacc
      601 ggttgttgca cggcgcaatg agcgattgcg gacaattttc caattgacga tgggttacgg
      661 gtgcggagat acttccggga tggcggttga atttcctatc cgtctcatcg cggcacgaaa
      721 acgccgccgt acggccgggt cggaaatgat cgcgaaggcc gcgccgagac cggggatatt
      781 gaaggtcttg gacggcgcca tcagggtgat gctgcgtgcc gcgacctcgg gtgaaaggct
      841 ggcgagaacg cggtggcggc aggccggatc gagcaccagg ccgttgtgga tctcgtccga
      901 gcagaccacg agatcgtgct tctctgccag cgccgcgatc tgccacaact cgtggcggtg
      961 cgctgtgcca cctggcctgt atgggcggca tcggctggaa gtcagcgacg ccgcgctggc
     1021 gctgatcgcc gaagccggct tcgacccggt gttcggcgcg cggcccttga agcgcgcgat
     1081 tcaggagcgc atcgagaatc cgctttcgcg gctaattctt gacggcacgc tgggcgccaa
     1141 ggatcgtgtg aaggtcgaag cgacgaaggg gcagatcagc tttgtgaagg cctgagcgcc
     1201 tgctgcgttc gccgcccgca aacggcgaat ccggcggaat ggatctcgtg ccattcccgg
     1261 cgccgtgttg ccagcgccga ctttcggaca gcatcgcagg cacccgaagg cggccggaca
     1321 tggtggcttg cttcgatagc tgtatgcgca tacagttacc catcccgatc ccccgatcag
     1381 gatgcgcatg cccacccaag ccgcaaagcc ctgccaagag gcgagcgtct atcaacgccg
     1441 gcgcccggaa cgaacgctgc tctaccacct cgtgcaagcg cacctcgcca cctggctggc
     1501 gctgcaggac gacggtgagg gtcgctcagc ctcagcccca gcgctcgtcg aacgcgaatt
     1561 ccgccgctac ctcgactgcg gcatcctcgc ccacggcttt gcccgtgccc gctgcgccga
     1621 ctgcggccac gatttcatcg tcgcctactc ctgcaagggc cgaggcgtct gcccttcgtg
     1681 caccactcgg cgcatggtcg aaaccgccgc ccatctggtc gatcacgtct ttcccgccct
     1741 gccggtgcgg cagtgggtgc tctcgctccc gaaacggctg cgctaccacc tcgatgacgc
     1801 caaactgcaa agcgccgtgc tgcacagctt tctgcgcgcc atcgaacagg gactgcggca
     1861 aagcctgccg gagggcgacg gcacggcacg catcggcgcg gtcgtcttca tccaccgctt
     1921 tggcgcgctg atgaacgccc accttcactt ccatgtcatc gcgatcgatg gggtgttttt
     1981 tggggaggag accgcgagtc tgcgtttcga ggagacgcac ctcagcgcgc aagcgctggc
     2041 gcgactgcaa tcgacgctgc gcaaacggat cgtcagcctt ttcgttcggc gcggcctgct
     2101 cgattcggcc gagggcgaag ccctgcgccg ctgcgcgcac ggcggcggct tctcgctcga
     2161 cgctggcgtg cgcatcgaag gggacgaccg gcaagggcgg gagcgcctgc tgcgctactg
     2221 cgcccgcccg gccttcgcgc aggaacggtt gcggcagatc gatgcggagc acctcgtcta
     2281 tgagagcccc aagccgggac cgggcgggcg cgtcagccag atcctgacgc ccctcgaact
     2341 gctcgatcgc ctcgccgcgc tgatcccgcc gccccggcgc caccggcatc gttactacgg
     2401 cgtgctggcg ccgaatgccc cccttcggtc ggcggtcacg gcactggcag cggcgccgaa
     2461 gccaccctcg gtcgaggccg atgccgtggc ggaggaaagt gcgatccgtc gggcggcgcg
     2521 ctatgcctgg gcgctcttgc tggcgcgcat ctacgaggtg ttcccgctca cctgcccaaa
     2581 gtgcggcggc gcgatgcgga tcatcgcctt catcgacgag ggcgaggcga tccgaaagat
     2641 cctccagcac ctgggcgagc cgatcgaacc gcccaagttg gccccggcgc gcggcccgcc
     2701 gctgtgggag gcggcgggac agggccacgc cgatccgctg gcgcagtcaa tccccgagtt
     2761 cgagttcgat cagcggatcg cgtggtagcg gacggcggcg aacgccgccc gacgggaacg
     2821 ctcttgcccg aggcccgcaa tacgcccgac agagggcgta ttgcgggcct c
//
LOCUS       DAORUQ010000297         2864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_49742_length_2864_cov_5.862229, whole genome shotgun sequence.
ACCESSION   DAORUQ010000297 DAORUQ010000000
VERSION     DAORUQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49742_length_2864_cov_5.862229"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1059)
                     /locus_tag="PLW13_15730"
     CDS             complement(61..1059)
                     /locus_tag="PLW13_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY71879.1"
                     /translation="MEHRNLGRSGLKVSAIGLGTHYFGWLHDEATSVAIIGHALELGI
                     NFIDTADVYDMGRSEEFVGKAIRGRRADLVIATKFGWPMGGGPSGAPVDPRPNARGGS
                     RHNVIRAAEASLKRLGTDYIDLYQIHFPDPSTPIEETLRALDDLVRAGKVRYTGCSNF
                     AAWQVSEAMWTSRCHNLNAFATSQPLYNLLQREIEQDHVPACDAHGLGIIPWGPLAGG
                     FLTGKYQRGQPYPADSLLATRPMAYEQITTEANWTKLERLEAFAHARGRRVGELAIAW
                     LLARPRVCSVIAGASRATQLEEHVAAGQWKLDASELLEIDAICAGDASAGHRFSLR"
     gene            complement(1093..>2864)
                     /locus_tag="PLW13_15735"
     CDS             complement(1093..>2864)
                     /locus_tag="PLW13_15735"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQY71880.1"
                     /translation="TPANWTSIATGAWPGTHGISDYNVRRPGDPLDRAHMAFHSPDVK
                     AEYVWNAIARAGKKSVVVNFVTTWPPVVDNGIQIGGSGCDINQWFHPNLALATRENGI
                     APDTVRLDEDGLGTGEAAPGVFAASACGGLFCTASFQPDSPVPVRIAPGAPEGWSNLP
                     AASRLLAAELQVRACGGRYPLTPPTWQLLVIDSHGEGFDRVLVCEGKDAAVPLAQLRA
                     GEWSAILHHTFQTDAGPERCAFALKLLELSPDARDLRLYHSSFCALEGWSSPASLASE
                     IVSTRGLPNPDSGFFGYDKGWFGADTLLEEIEMQRQWYADACAHVMKNEPWDLFVMRY
                     HLPDTSWHSIPHVLDPRLATDAEERRRHEALELGIYQACDRLARDLIACVDERETLLA
                     LISDHGAKPAGHPGIDANAILRDAGLLARHTGGTIDWSRTRAIARPVCWIHINLQDRD
                     PDGIVRAGEEYREVQDAIIRALTEYVEPGSGRKPVLFALRKEDARFLNIYGEQAGDVV
                     YALKHDHGHQHGPFLPTADWQGGSLRGLFALSGPGIRRGAQIERNVWCIDLVPTICHL
                     TGWPVPRDTEGAVIYQAFDEPGS"
BASE COUNT          442 a          944 c          996 g          482 t
ORIGIN      
        1 tgaagctcgc gcccgcgctg ctcaccgagc tggagcagca ggccaggcgg gctttgccgc
       61 tcacctcagg ctgaagcggt gtccggcgct ggcgtcaccg gcgcagatgg catcgatctc
      121 gagaagttca gaggcatcga gcttccattg tccggccgcg acgtgctctt cgagttgcgt
      181 tgcccggctg gctcccgcga tcaccgagca tacgcgtggc cgtgccagca gccaggcgat
      241 cgccagttcg cccacgcgac ggccgcgcgc atgcgcgaat gcctcgagcc gctccagctt
      301 ggtccagttg gcttccgtgg tgatttgctc gtaggccatc ggcctggtcg cgagcaggga
      361 gtcggcgggg tagggctggc cgcgctggta cttgccggtc agaaatccgc ccgccagcgg
      421 gccccacgga atgatgccga gtccgtgggc gtcgcacgcc gggacgtggt cctgctcgat
      481 ctcgcgctgg agcaggttgt acagcggctg cgaggtcgcg aaggcgttga ggttgtggca
      541 gcgcgaggtc cacatcgcct ccgaaacctg ccaggcggca aaattgctgc agccggtgta
      601 gcgcaccttg cccgcgcgca cgagatcgtc gagcgcgcgc agcgtttcct cgatcggcgt
      661 cgagggatcg ggaaaatgga tctggtagag atcgatgtag tcggtgccga gccgcttcag
      721 gctcgcttcc gccgcgcgga tcacgttgtg acgcgaaccg ccgcgcgcgt tgggacgcgg
      781 atcgaccggc gcgcccgacg ggccgccgcc catgggccag ccgaacttgg tcgcgatcac
      841 gaggtccgcg cgccgccccc tgatcgcctt gccgacgaac tcctccgagc gccccatgtc
      901 gtagacgtcg gcggtatcga tgaaattgat gccgagctcg agcgcatgcc cgatgatggc
      961 aaccgaagtt gcctcgtcgt gcagccagcc gaagtagtgg gtgcccagcc cgatggccga
     1021 aaccttcagc cccgatctgc ccagattgcg atgctccacg cgcgtgctcc ttgcggtcga
     1081 tgatcgtcgt tttcagctgc ccggctcgtc gaatgcctgg tagatcaccg cgccctcggt
     1141 atcgcgcggc accggccagc cggtcaggtg gcagatcgtg gggacgaggt cgatgcacca
     1201 cacgttgcgc tcgatctgcg cgcccctgcg gataccgggg ccgctgagcg cgaacaggcc
     1261 gcgcagcgat ccgccctgcc agtcggcggt ggggaggaag gggccgtgct ggtgaccgtg
     1321 gtcgtgcttg agcgcataca ccacgtcgcc ggcctgctcg ccgtagatgt tgaggaagcg
     1381 tgcgtcttcc ttgcgcagcg cgaacagcac cggcttgcgc ccggagccgg gttcgacgta
     1441 ctcggtgagc gcacgaatga tcgcatcctg cacctcgcga tactcctcgc cggcacgcac
     1501 gatgccgtcc gggtcgcggt cctgcagatt gatatggatc cagcacacgg gtcgcgcgat
     1561 cgcgcgcgtg cgcgaccagt cgattgttcc cccggtgtgt cgggccagca gcccggcatc
     1621 gcgcagaatc gcgttggcat cgatgccggg atggccggcg ggcttggcgc cgtggtccga
     1681 gatcagcgcc agcaatgtct cgcgctcgtc gacgcacgcg atcaggtcgc gcgcgaggcg
     1741 atcgcaggcc tggtagatgc cgagctccag cgcttcgtgc cggcggcgtt cctcggcgtc
     1801 agttgcaagc ctcgggtcga gcacgtgcgg gatcgaatgc cacgaggtat cgggcaggtg
     1861 gtagcgcatc acgaacaggt cccacggttc gttcttcatc acgtgggcgc aggcatcggc
     1921 ataccactgc cgttgcatct cgatttcctc gagcagcgtg tcggcgccga accatccctt
     1981 gtcgtagccg aaaaacccgc tgtcgggatt gggcaggccc ctggtggaga cgatctccga
     2041 ggccagcgat gcgggcgagg accagccctc gagcgcgcag aaggaactgt ggtagagacg
     2101 caggtcgcgg gcatccggcg acagctcgag cagcttcagg gcaaacgcac agcgctcggg
     2161 tcccgcgtcg gtctggaacg tgtgatggag tatggcgctc cattcgccgg cccgcagttg
     2221 cgcgagcggc acggccgcat ccttgccctc gcacaccagc acacggtcga agccctcgcc
     2281 gtgcgagtcg atgaccagca gctgccaggt cggtggcgtc agcgggtagc ggccgccgca
     2341 ggcgcgcacc tgcagctccg cggcgagcag cctcgatgcc gcgggcaggt tgctccaccc
     2401 ctcgggcgcg cccggcgcga tgcgcacggg taccggcgaa tccggttgaa agctcgccgt
     2461 gcagaacagg ccgccgcagg cgctcgcggc aaacaccccc ggcgccgctt cgccggtgcc
     2521 gagcccgtct tcgtcgaggc ggaccgtgtc tggcgcgatg ccgttctccc gggtggcgag
     2581 cgcgaggttc gggtggaacc actggttgat gtcgcagccg gagccgccga tctggatgcc
     2641 attgtccacg accggcggcc aggtggtgac gaaattgacg acgaccgact tcttgccggc
     2701 acgcgcaatc gcgttccaga catattcagc tttcacatcg gggctgtgaa aggccatgtg
     2761 agcgcgatcc agcggatcgc cgggacgacg cacgttgtag tcggaaatgc cgtgcgtccc
     2821 cggccatgcg ccggtggcga tcgatgtcca gttggctggt gttg
//
LOCUS       DAORUQ010000298         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_50303_length_2845_cov_10.094982, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000298 DAORUQ010000000
VERSION     DAORUQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50303_length_2845_cov_10.094982"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLW13_15740"
     CDS             <1..415
                     /locus_tag="PLW13_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71881.1"
                     /translation="TQAADSPAGAQSAPAPASRMSPRHMDPATMTRRMTTNLGLSPEQ
                     VTEVEQINQRFATQFEQQRKQQEAMRAAHQEAMGKIAAERDAALKKVLSEEQYGRHVA
                     HRQEMQERRRAHRGPGGPGTGPRAAEAPAPTPPTQ"
     gene            485..1885
                     /gene="sthA"
                     /locus_tag="PLW13_15745"
     CDS             485..1885
                     /gene="sthA"
                     /locus_tag="PLW13_15745"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HQY71882.1"
                     /translation="MDAFKFDLLVIGSGPAGESAALNAAKHGLRAAVIEDRRMVGGGC
                     THSGTIPSKALRHAVKDLTRYNTNAIFRAIGEPSRISFQTLLQNAGTVIRRQVAMRSQ
                     YYAKNRIAMYFGVASFIDANTVRVTLPDGAVEILRAPKVVIATGSRPYRPADIDFAHP
                     TVFDSDTILAMPHTPRRLLIYGAGVIGCEYASIFSALGVKVDLINTRDQLLSYLDDEI
                     SDALSYHLRTQGAVIRHGEEYDRLEASAHGVTLHLRSGKRLQGEALLWCNGRTGNTDR
                     MELHNIGLQPDSRGLLNVDARYETSVPGVFAVGDVIGAPSLASAAYDQGRSAAAAARG
                     KEHTRHVDDTPTGIYTLPEISSVGATERELTAARIPYEIGRAFFKETARAQISGEDVG
                     MLKLLFHIDTLEILGVHCFGAEATEIIHIGQAIMKQPAPRNTVRYFIETTFNYPTMAE
                     AYRIAALNGLNRLTSY"
     gene            complement(2298..>2845)
                     /locus_tag="PLW13_15750"
     CDS             complement(2298..>2845)
                     /locus_tag="PLW13_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase 2"
                     /protein_id="HQY71883.1"
                     /translation="GLRVPLLATPFEAMVWAVTGQQISVAAATAVRRRLIEATGIRHS
                     VGLLCHPDPARLARMGEAQLRAAGLSQAKAATVNLLSRGAADGTLPFEAWADAPPVAE
                     IRARLAAIRGIGPWTIEYTLLRGFGHLDGSLHGDVAVRRKLQLLLGREEKISAAETGR
                     WLAQFTPWRALVAAHLWAWHP"
BASE COUNT          497 a         1010 c          928 g          410 t
ORIGIN      
        1 cacacaggcc gccgattccc cggccggggc gcaatccgcg cctgctcctg cgtcgcgcat
       61 gagcccgcgg cacatggatc ccgccacgat gacccggcgc atgaccacca acctggggct
      121 gtcgccggag caggtaacgg aggtggagca gatcaaccag cgcttcgcca cgcagttcga
      181 gcagcagcgc aagcagcagg aagccatgcg tgcggcgcac caggaggcca tgggcaagat
      241 cgccgcagaa cgcgatgcgg cgctgaaaaa ggtgctgagc gaggagcagt acgggaggca
      301 cgtcgcccac aggcaggaga tgcaggagcg gcgccgcgcg catcgcggtc ccggcggccc
      361 gggcacggga ccgcgcgctg ccgaggcacc cgcgccgacg ccgcccacgc agtaatcacg
      421 cgcgccccgt tggacagccg ccgggcagag cgtctatata atcggttttg cccggcggcc
      481 cgtgatggat gcattcaaat tcgatttgct ggtgatcggc agcggcccgg cgggcgaatc
      541 cgcggcgctg aacgcggcca agcacggcct gcgcgccgcg gtgatcgagg accgtcgcat
      601 ggtcggcggc ggttgcaccc acagcggcac gatcccgtcc aaggcgctgc gacacgcggt
      661 caaggacctg acgcgctaca acaccaacgc gatattccgc gccatcggcg agcccagccg
      721 catctccttc cagaccctgc tgcagaacgc cggcacggtg atccgccgcc aggtcgccat
      781 gcgctcgcag tactacgcga agaaccgcat tgcgatgtat ttcggggtgg catcgttcat
      841 cgatgccaac acggtgcgcg tgacgctacc cgacggcgcc gtcgagatcc tgcgcgcacc
      901 gaaagtcgtg atcgcgaccg gctcgcgtcc ctaccgtccc gcggacatcg acttcgcgca
      961 ccctacggta ttcgacagcg ataccatcct cgccatgccg cacacgccgc gtcgcctgct
     1021 gatctacggc gcgggcgtca tcggctgcga atacgcctcg atcttctccg cgctgggggt
     1081 caaggtcgac ctgatcaaca cacgtgacca gctgctctcc tatctcgacg acgagatctc
     1141 cgatgcgctg tcgtatcacc tgcgcacaca gggcgcggtg atccgccacg gcgaggaata
     1201 cgatcggctc gaagcctcgg cgcacggcgt cacgctgcac ctgcgctcgg gcaagcgcct
     1261 gcagggcgag gcgttgctgt ggtgcaacgg ccgcactgga aataccgacc gcatggagct
     1321 gcacaacatc ggcctgcagc ccgattcgcg cggcttgctg aacgtcgatg cgcgctacga
     1381 aacctccgtt cccggcgtat tcgccgtcgg cgatgtgatc ggtgccccca gcctcgcgag
     1441 cgcggcctac gaccagggcc gctcggccgc ggcagcggcg cgcggcaagg aacacacgcg
     1501 ccacgtcgac gacacaccca ccggcatcta cacgctgccc gagatcagct cggtgggcgc
     1561 caccgagcgc gaactcaccg cggcacgcat cccctacgag atcggccgcg ccttcttcaa
     1621 ggagacggcg cgcgcgcaga tcagtggcga ggacgtgggc atgctgaagc tgctgttcca
     1681 catcgacacg ctggagatcc tcggcgtgca ctgcttcggc gccgaggcca ccgagatcat
     1741 ccacatcggc caggcgatca tgaagcagcc ggcgccacgc aacaccgtgc gctatttcat
     1801 cgagaccacg ttcaactacc ccacgatggc cgaggcctac cgcatcgccg cactgaacgg
     1861 cctgaaccgc ctgacgagct actgagtggc cccgccgctc ccatcaaact ggcccctctg
     1921 cgagaactgc ccccccgtag gtccggattc atccggacag tgtccactcg tagggtggtg
     1981 tcgcaaaccc atccgtgctc tggatagcgg cctgacaatc cggtgtatcg cgagtcatcg
     2041 gcgtccgagc acctcaccgt ggtggaagcc gtcaagaagg cgcgtattcc gggattgact
     2101 cgatgcgctg cgagctcaac cgagcggctg tgctgcccgc tgttggcttg cggggccttg
     2161 cgccgcagga cgcggcgcgg agctacaggg acgtactcgt gcgtgccccg caggacaatg
     2221 gcgggcagca cagccagcga cgcaccaggc cgctcgatcg caatccaatg gttttacaac
     2281 accctcttga tgcgggccta cgggtgccag gcccagaggt gcgcggccac cagcgcgcgc
     2341 cacggcgtga actgcgccag ccagcgcccg gtctctgccg cggagatttt ttcttcccgg
     2401 cccagcagca gttgcaactt gcgccgcacc gccacgtcgc cgtgcagcga gccgtcgaga
     2461 tggccgaagc cgcgcagcag cgtgtactcg atggtccacg ggccgatgcc gcggatcgcc
     2521 gcgaggcgtg cgcggatctc cgcgacgggc ggtgcgtcgg cccaggcctc gaatggcagg
     2581 gtgccgtcgg cagcgccacg gctcagcagg ttaacggtgg ctgccttggc ctgtgacagc
     2641 ccggcggcgc gcaattgcgc ttcccccatg cgcgccagcc gcgcgggatc gggatggcac
     2701 aacaggccga ccgagtggcg gatgccggtg gcctcgatga gccggcggcg caccgcggtg
     2761 gccgcggcca cgctgatctg ctgtccggtc acggcccaca ccatcgcttc gaacggtgtg
     2821 gccagcaacg gcacgcgcag gccgg
//
LOCUS       DAORUQ010000299         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_50400_length_2842_cov_5.073197, whole genome shotgun sequence.
ACCESSION   DAORUQ010000299 DAORUQ010000000
VERSION     DAORUQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50400_length_2842_cov_5.073197"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..970)
                     /locus_tag="PLW13_15755"
     CDS             complement(113..970)
                     /locus_tag="PLW13_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71884.1"
                     /translation="MNTPDIQLTDQVAVVTGAGAGIGRAIALACAAVGADVVIGDIVP
                     ERCEEVAARVREMGRAALAVPTDVCDTEQVRALIDSAASRFGRIDILVNNAGGVSPRP
                     FIEQSERSWRRHIDLNLVSMLAATSAAAPLMIRGGRGGSIINVSSIEGSRAAPNFAVY
                     GACKAAMLNFTRTMALELAEHGIRVNVIAPDYTVTPGLRGNHTGPVDPASWIRPSDQQ
                     EDATARRIPLGRAGTDTECGNVAVFLASAMSSYVTGAVIPVDGGTWASSGWVRNKAGN
                     WTLMGDVIS"
     gene            complement(1014..2156)
                     /locus_tag="PLW13_15760"
     CDS             complement(1014..2156)
                     /locus_tag="PLW13_15760"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY71885.1"
                     /translation="MDRHEELLAAARAGTGLEDFGDDSFLEGLQVLVRSLREEAQLNA
                     TGEHVLRQRIIGHLEQRLEIEDWYRRHPEIDEVQIKAPLIGVSLPRTGSTALSFLLAE
                     DPDARSLRRWESSRPCPPPSTVTPPDPRIVQAQAGAAARREGVKGHVPESATGPAECQ
                     ELMALDFRSHLFQAFARIPGYSAWLLEADLSSTYRYERRVLKLLQWGCPDKPWRLKCP
                     THVLFLEHLDRAFPDARFVMTHRDPTDVIVSVAHLYADYIGKFSDRVDLHDVGRLNVQ
                     HWSLGMERALAFRDKGNEARFHDIDFRAMQRDPVGAVRRLYEWLGEPVSASFEAGMSR
                     WWQENAQNREPGAHPEAATYGLDLDQVRPLFANYTARMASWTGGRS"
     gene            complement(2205..>2842)
                     /locus_tag="PLW13_15765"
     CDS             complement(2205..>2842)
                     /locus_tag="PLW13_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71886.1"
                     /translation="DKRTAVRLDVDLHMVTPAWVQDIPVEKVARMSAADAIEAANMGI
                     GWRFEHLFRARGVFHVDGLSREFKGVGLRVKRQSIRPLGGFRGHCWQSAVFPDGRAFG
                     YITYPPREDGIAYNEGYVFQDGTMYPARATRIPWLRRIVGEGDDASLELESDLGITRI
                     EGVTALSTFRVGNPDIGGLNLQQGGARYTWDGQTAYGMIERSSHESLTTIG"
BASE COUNT          452 a          984 c          933 g          473 t
ORIGIN      
        1 gacaccgctt cgggttgcag gaattcctcc ggactgtcga tacggtcgaa gggcaaggtg
       61 gcgttgattt tcatatcggc tcatccgcgc gaggagatcc gcgataccgg tctcaggaaa
      121 tgacatcgcc catcagcgtc cagttgccgg ccttgttacg cacccagccg ctggaggccc
      181 aggttccccc gtcgacggga atgacggcgc cggtcacgta actgctcatc gcggaagcca
      241 ggaacacggc gacgttgccg cactccgtgt ccgttcccgc gcgccccagc gggatgcgcc
      301 gggccgttgc atcctcctgc tgatcgctgg gcctgatcca gctcgccggg tcgaccgggc
      361 ccgtgtggtt gccgcgcagc ccgggcgtga ccgtgtaatc gggggcaatg acattgacgc
      421 gaatgccgtg ctccgcgagt tccagcgcca tcgttcgcgt gaagttgagc atggccgcct
      481 tgcacgcccc gtagacggcg aagttcggcg cggcccgcga cccctcgatg ctgctgacgt
      541 tgatgatgga gccgccgcga ccgccgcgga tcatcagcgg agccgcggca gaggtggccg
      601 ccagcatgct gaccaggttg agatcgatgt ggcgccgcca ggaacgttcg gactgctcga
      661 tgaacggccg cgggctcaca ccgccggcgt tgttcaccag aatgtcgatg cgcccgaagc
      721 gggaggccgc gctgtcgatc agggcgcgca cctgctccgt gtcgcataca tcggttggca
      781 ccgccagggc ggcgcggccc atttcgcgca cgcgcgcggc gacttcctcg caacgctcgg
      841 ggacgatatc gccgatgacc acgtcggcgc cgaccgccgc acaggccagc gcaatcgccc
      901 tgccgatgcc ggcccctgcg ccggtgacga cggcaacctg gtccgtgagc tggatatccg
      961 gtgtattcaa ttcgatctcc ggtggatgaa catcgacgct cggaaagtgt ccttcagctg
     1021 cgcccccctg tccacgacgc catgcgcgcc gtgtagttcg cgaacagcgg gcggacctgg
     1081 tcgaggtcca gcccgtaggt cgccgcctcg ggatgcgcgc ccggttcgcg gttctgcgcg
     1141 ttctcctgcc accagcggct catgccggcc tcgaagctcg cgctcacggg ctcgccgagc
     1201 cactcgtaca gccggcggac cgcgccgacg ggatcgcgct gcatcgcgcg gaaatcgatg
     1261 tcatggaatc gcgcctcgtt gcccttgtcg cgaaacgcca gtgcccgttc catgcccagc
     1321 gaccaatgct gcacgttgag cctgcccacg tcgtgcaggt ccacccggtc actgaacttg
     1381 ccgatgtagt cggcgtagag gtgcgccacc gagacgatca cgtccgtggg atcgcggtgc
     1441 gtcatcacga aacgcgcatc cggaaacgcg cggtccaggt gctcgagaaa cagcacgtgc
     1501 gtggggcact tcaggcgcca cggcttgtcg gggcatcccc actgcagcag cttgagcacc
     1561 cggcgctcgt agcgataggt cgagctgagg tccgcctcca gcagccacgc ggaataaccc
     1621 ggtatccggg cgaacgcctg gaacaggtgg ctcctgaaat cgagcgccat cagctcctgg
     1681 cactccgcgg gtccggtggc cgattcgggc acgtgtccct tgaccccttc cctgcgggct
     1741 gccgccccgg cctgcgcttg cacgatgcgc ggatcgggcg gcgtcaccgt cgagggcggc
     1801 ggacagggtc gggacgattc ccagcggcgc agcgaacggg cgtcgggatc ttcggccagc
     1861 aggaacgaca acgcggtcga gccggtgcgc ggcaggctga cgccgatcag gggcgccttg
     1921 atctggacct cgtcgatttc cgggtgccgg cgataccagt cctcgatctc caggcgctgc
     1981 tcgaggtggc cgatgatgcg ctggcgcagc acgtgttcgc cggtggcatt gagctgcgcc
     2041 tcctcgcgca gcgaacgcac cagaacctgc agcccctcca gaaacgagtc gtcgccgaaa
     2101 tcctccaacc ccgtccctgc gcgcgccgcg gccagcagtt cctcgtgtcg atccattgat
     2161 cgcctcgtgt gccggcgcaa taccggcagt gctttggagc ccgatcagcc gatcgtcgtc
     2221 aggctttcgt gcgacgagcg ctcgatcatg ccgtaagccg tctgtccgtc ccaggtgtag
     2281 cgtgcaccac cctgctgcag gttcagaccg ccgatgtcgg gattgccgac gcgaaaggtc
     2341 gacagcgccg tcacgccctc gatcctggtg atccccagat cggactcgag ctccagcgaa
     2401 gcgtcgtcgc cctcgcccac gatacggcgc agccacggaa tccgggtggc gcgggccggg
     2461 tacatcgtgc cgtcctggaa gacataaccc tcgttgtagg cgatgccgtc ctcgcgcggc
     2521 gggtacgtga tgtagccgaa cgcgcgcccg tcgggaaata ccgccgactg ccagcagtgc
     2581 ccgcggaacc cgcccagcgg acggatgctc tggcgtttga cccgcagccc cacgcccttg
     2641 aactcgcggc tcaggccatc gacatggaaa acgccgcggg cgcggaacag gtgttcgaag
     2701 cgccagccta tgcccatgtt ggcggcttcg atggcatcgg ccgcggacat ccgcgccact
     2761 ttctcgaccg ggatgtcctg cacccaggcc ggcgtgacca tgtgcagatc gacgtccagg
     2821 cgtaccgcgg tgcgcttgtc gg
//
LOCUS       DAORUQ010000300         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_50425_length_2841_cov_5.762742, whole genome shotgun sequence.
ACCESSION   DAORUQ010000300 DAORUQ010000000
VERSION     DAORUQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50425_length_2841_cov_5.762742"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLW13_15770"
     CDS             complement(<1..841)
                     /locus_tag="PLW13_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQY71887.1"
                     /translation="MNAPRGRILLVDDDPSLMRLLVLRLEAEGFEVVTAASGTEALQR
                     LRGRPVELMISDLRMEGMDGIELFEHVQHYYPGLPVIIITAQGSIPDAVAATQKGVFG
                     FLTKPIEREQLKSLIERALSVSAASRPPATQGGWRDHVQSRSQVMERLLQTAQQVASS
                     NISVLISGGSGSGKELLALAIHRSSARCDGPFIPLNCGALPEALLESELFGHVKGAFT
                     GAVSDHVGLFRAAEGGTLFLDEIGDMPLALQVKLLRVLQEKEVRPVGSTRSFGIDVRV
                     LSAT"
     gene            complement(838..1386)
                     /locus_tag="PLW13_15775"
     CDS             complement(838..1386)
                     /locus_tag="PLW13_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71888.1"
                     /translation="MRILALLLAGVLLAACQATPPQAPAPAPAMPQAPDPDSVMLSQW
                     LRDADSALQLSAREARAQLAAMQADSPTPAQRYRLALLHQQLGEWNSWALARDTMRAL
                     RADETLPPDVRRLAGLLEAFNQSRLNAQQQRTELRKSLAEQEALLVQRQAEVQALEEK
                     IRSLTTLEDSMHQRREEQGGQR"
     gene            complement(1383..>2841)
                     /locus_tag="PLW13_15780"
     CDS             complement(1383..>2841)
                     /locus_tag="PLW13_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQY71889.1"
                     /translation="IRSRVFRDMRWRPRSILQLILLGFLTVVAPLSAAVVLAVQTLVE
                     QAVDNARLNERTVTLTRSTQQLQGDLLDLERRTRQYATLGDASLLRLVLEGFDELQAT
                     LATIGERLDAGGAPAVERLGEQLRELQHELTLVPGDAARLETALALFDPISREAQATG
                     HSSRLQVDAELAAQLLAADATRTRMILIAGALALLTVPLALLFTYLINQPIRQLEQVI
                     RQLGSARAGGAIRIQGPREMQLLGRQLSWLRDQLAELDAQKQQFLRHISHELKTPLAS
                     LCEGADLLAEGVVGPALNPAQREIVSIVQQNSKELQRLIENLLDYNQLLHRAEVRPCE
                     VALGPLVAEVVDSHRLGLKRHGLVAVLRDEQALWVMDAGKLRTALDNLVSNAANYASQ
                     DSEVLIHWRVHRDMLEIDVANHGPLIPADEAERIFEPFFQGSTGRRGAIKGSGIGLSV
                     ARECIESQGGSLRLVPRKPYSTCFRITLPRLEEPA"
BASE COUNT          404 a         1016 c          978 g          443 t
ORIGIN      
        1 gggtcgccga cagcacgcgc acgtcgatgc cgaagctgcg cgtgctgcca accggccgga
       61 cttccttctc ctgcagcacg cgcagcagtt tcacctgcag cgccagcggc atgtcgccga
      121 tctcgtcgag gaacagcgtg ccgccctcgg cggcgcggaa caggcccacg tggtcgctga
      181 cggccccggt gaaggcaccc ttcacgtgac cgaacagctc cgattcgagc agagcttcgg
      241 gcagcgcacc acagttcagg ggaatgaacg gcccgtcgca gcgcgcgctc gagcggtgta
      301 tcgccagcgc cagcagttcc ttgccgctgc cgctgccccc cgagatcagc acgctgatgt
      361 tgctggacgc gacctgctgc gcggtctgca gcaggcgctc catcacctgg ctgcgcgact
      421 ggacgtgatc gcgccagccg ccctgcgtcg ccggcgggcg gctcgccgcc gacacgctga
      481 gcgcgcgctc gatcaggctc ttcagctgct cgcgctcgat cggcttggtg aggaaaccga
      541 acaccccttt ctgggtcgcg gcaaccgcgt cggggatcga tccctgggcc gtgatgatga
      601 tcaccggcag gccggggtaa tagtgctgga cgtgctcgaa cagttcgatg ccgtccatgc
      661 cttccatgcg caggtccgag atcatcagtt cgaccgggcg cccgcgcagg cgctgcagcg
      721 cctcggtccc gctggcggcg gtgaccacct cgaagccctc ggcctccagg cgcagcacca
      781 gcagccgcat caggctcggg tcgtcatcga ccagcaggat ccgtccgcgc ggggcgttca
      841 ccgctgcccg ccctgctctt cgcgccgctg gtgcatgctg tcctcgaggg tcgtgagcga
      901 gcggatcttc tcctccagcg cctgcacctc ggcttgcctc tgcacgagca gcgcctcctg
      961 ctcggcaagg ctcttgcgca gttccgtgcg ctgctgctgc gcgttgaggc gcgactggtt
     1021 gaaggcctcc agcaggccgg ccaggcggcg cacgtccggc ggcagcgtct cgtcggcgcg
     1081 cagcgcacgc atggtgtcgc gcgccagcgc ccacgagttc cactcgccga gttgctggtg
     1141 cagcagggcc aggcgatagc gctgcgcggg cgtcggggaa tccgcctgca tcgcggcgag
     1201 ctgcgcgcgc gcttcccggg ccgacagttg cagtgcgctg tcggcatcgc gcagccattg
     1261 cgacagcatg acgctgtccg ggtccggcgc ctgcggcatt gccggcgcgg gcgccggcgc
     1321 ctgcggcggc gtggcctggc atgcggccag cagcacgccg gcgagcagca gggcgaggat
     1381 tctcatgcgg gctcctccag gcgcggcagg gtgatgcgaa agcaggtgct gtagggtttg
     1441 cgcggcacca gtcgcagcga accgccctgc gactcgatgc attcgcgcgc cacgctcagg
     1501 ccgatgcccg agcccttgat cgcaccgcga cggcccgtcg agccctggaa aaacggctcg
     1561 aagatgcgct ccgcctcgtc ggccgggatg agcggtccgt ggtttgccac gtcgatctcg
     1621 agcatgtccc ggtgcacgcg ccagtggatc agcacctcgc tgtcctggct cgcgtaattc
     1681 gccgcgttgg agaccaggtt gtcgagcgcg gtcctgagct tgcccgcgtc catcacccac
     1741 agcgcctgct cgtcgcgcag cacggccacg aggccgtggc gtttgaggcc caggcgatgc
     1801 gagtcgacaa cctcggcaac cagcggcccc agcgccactt cgcacggcct cacctcggcg
     1861 cggtgcagaa gctggttgta gtcgagcagg ttctcgatca gccgctgcag ctccttgctg
     1921 ttctgctgca cgatggacac gatctcgcgc tgcgcggggt tcagcgccgg acccacgacg
     1981 ccttcggcca gcaggtcggc tccctcgcac aggctggcca gcggggtctt cagctcgtgc
     2041 gagatgtggc gcaggaactg ctgcttctgc gcatcgagct ccgccagctg atcgcgcagc
     2101 cacgagagct ggcggcccag cagctgcatt tcccgcggcc cctggatgcg gatggctccc
     2161 ccggcgcggg cactcccgag ctggcggatg acctgctcga gctggcggat cggctggttg
     2221 atcaggtagg tgaacaacag cgcgagcggc accgtcagca gcgccagggc gccggcgatg
     2281 aggatcatgc gggtgcgtgt ggcgtccgcc gcaagcaact gcgccgcgag ctccgcgtcg
     2341 acctgaagcc tgctgctgtg cccggtagcc tgcgcctcgc ggctgatcgg gtcgaacagc
     2401 gccagggccg tctccaggcg cgcggcatcc ccgggcacca gcgtcaactc gtgctgcaac
     2461 tcgcgcagct gctcgcccag gcgttcgacc gccggggcgc ccccggcgtc gaggcgctcg
     2521 ccaatggtgg ccagtgtcgc ctgcagctca tcgaacccct ccagcaccag gcgcagcagg
     2581 gacgcatccc cgagcgtggc gtactggcgc gtgcgccgct ccaggtcgag caggtcgccc
     2641 tgcaactgct gcgtcgagcg ggtcagggtg acggtgcgct cgttgaggcg ggcgttgtcc
     2701 acggcctgct ccaccagcgt ctgcacggcc agcaccaccg cggcgctcag cggcgcgacc
     2761 accgtcagga agcccagcag gatcaattgc aggatcgaac gggggcgcca gcgcatgtcg
     2821 cggaataccc ggctccggat g
//
LOCUS       DAORUQ010000301         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_50711_length_2831_cov_5.495317, whole genome shotgun sequence.
ACCESSION   DAORUQ010000301 DAORUQ010000000
VERSION     DAORUQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50711_length_2831_cov_5.495317"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PLW13_15785"
     CDS             <1..1504
                     /locus_tag="PLW13_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71890.1"
                     /translation="SGDPERVLIIDGPVRIDARSLHAQASALAQTLAARFAPGSVISF
                     MLPNWHEAAVIYLATTLAGMVAHPVLPSLREHDLCFMLKDVESRMIFIPARFRGHESA
                     QMLTRVVQQLDTPPQVVVVRGDRGEHIAYDSLFSGVAPQPLPAPDPDAVRMIMYTSGT
                     TGSPKGVMHSHNSIHALIRQIGRHWLVEPGDRFLVAGPISHIGGSIYAFECPLLLGAT
                     AVLMERWDADEAIGHLVADRCTHFAGATPFLVQTLAAARRAQTRLPELKLFVCGGASV
                     PPSLICEAADYFEKAVVTRVYGSTEVPVTTVGVISRSDVAHAAETDGQAGIATIRLVD
                     GSGATAATGEVVAQGPQMLVGYVHPEDEASVFDAEGYYRTGDLGRWVDGDYLVIAGRI
                     KDIIIRNGENIAPKEVEDLLVAHPDIAEIAIVGLPDARTGERACAVIVPRGSARPDVA
                     SLRAYLDARGVAKFKMPEQVALWEALPKNEAGKVLKHQMRAALADASPRD"
     gene            complement(1554..2387)
                     /locus_tag="PLW13_15790"
     CDS             complement(1554..2387)
                     /locus_tag="PLW13_15790"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQY71891.1"
                     /translation="MTAAISEKLEASDISPLIASEVRLGKDALLSGEHATDLRELLER
                     RGVLVFPRIGFTDEEQVAFTRTLGTFAPEMRGEEIYKVTLDTRANASAEYLKGSLYWH
                     LDGTMNQVPILASLLSAKVLSPTGGDTEFCNTYAAWEMLAEEDKQQIEALRVVHSAWA
                     SLFYYDPEPSLAMTRQMMAIGEQELPLVWKHRSGRKSLVLGCTAHHVVGMSAMESTQL
                     LVRLREWATQPQFTYRHKWTVGDLVMWDNTGTMHRATPYDPAAGRLLHRTKLQGEEPF
                     E"
     gene            complement(2467..>2831)
                     /locus_tag="PLW13_15795"
     CDS             complement(2467..>2831)
                     /locus_tag="PLW13_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HQY71892.1"
                     /translation="AEGRLHWHGRLNDIIKTGGANVSPTEIDALLRECPGVKIATTVG
                     VPHETLGEIVVTCIVREQGATLDEPTVRAFVSARLSSYKVPRRVLFVQEADLELTASN
                     KVKTAPLRELAARRLADS"
BASE COUNT          445 a          972 c          924 g          490 t
ORIGIN      
        1 atccggggac ccggagcgcg ttctcatcat cgacggcccg gtgcgcatcg acgcccgcag
       61 cctgcacgca caggcatccg ccctggcgca gaccctggcg gcacggtttg cgccgggaag
      121 cgtcatctcc ttcatgctgc ccaactggca cgaagccgcg gtcatctacc tggcaacgac
      181 gctggcgggc atggtcgcgc acccggtgct gccatcgctg cgcgagcacg acctgtgttt
      241 catgctgaag gatgtcgaga gccggatgat cttcataccg gcgcggtttc gcggacacga
      301 gtccgcgcag atgctgacgc gggtcgtgca gcagctcgat acgccgccgc aggtggtcgt
      361 cgtgcgcggc gatcgcggcg agcacatcgc ttatgactcg ttgttctcgg gcgtcgcgcc
      421 gcagccgctg ccggcgcccg atccggatgc cgtgcggatg atcatgtata catccgggac
      481 caccggcagc cccaagggcg tcatgcacag ccacaattcg attcacgcgc tgatccgcca
      541 gatcggcagg cactggctgg tcgagccggg cgaccgattc ctcgtcgcgg gtcccatcag
      601 ccatatcggc ggctccatct acgcgttcga gtgcccgctg ctgctgggtg caacggccgt
      661 attgatggag cgctgggatg ccgacgaggc gatcggccac ctcgtggccg atcgctgcac
      721 gcattttgcc ggcgccacgc ccttcctcgt gcagacactc gccgcggcgc gacgcgcgca
      781 gacgcgtctt ccggagctca agctcttcgt gtgcggcggg gcctcggttc ccccgtcgct
      841 gatctgcgaa gcggcggact atttcgagaa ggccgtggtc acacgagtct atggctcaac
      901 cgaggttccg gtgacgacgg ttggcgtcat cagccgaagc gacgtcgccc acgcggcgga
      961 aaccgacggg caagccggga tcgccaccat caggctggtc gacggctccg gcgcgaccgc
     1021 cgcaaccggt gaagtcgtgg cgcaagggcc gcagatgctg gtgggctacg tccaccccga
     1081 ggacgaggcg agcgtattcg acgccgaggg ctattaccgc accggtgacc tggggcgttg
     1141 ggtcgatggc gactacctcg tcatcgccgg ccgcatcaag gacatcatca tccgcaacgg
     1201 cgagaacatc gcgcccaagg aagtggaaga cctgctggtg gcgcatcccg atatcgcgga
     1261 aatcgccatt gtcggtctgc ccgatgcccg gaccggcgag cgcgcctgcg ccgtcatcgt
     1321 gccgcgcggt tcggcacggc ccgacgtcgc cagcttgcgc gcctatctcg atgccagggg
     1381 ggtcgcgaag ttcaaaatgc cggaacaggt ggcgctatgg gaggcactgc ccaagaacga
     1441 ggccggcaag gtcctgaagc accagatgcg cgccgcgctg gccgatgctt cgccccgcga
     1501 ctgagcgcta gtgagttttt cgccagcctg ctgcggccgc tgctgcagcg ctcctattcg
     1561 aaaggctctt cgccctgaag cttggtccgg tgcagcaggc gcccggcagc cgggtcgtag
     1621 ggagtggccc ggtgcatcgt gccggtgttg tcccacatca ccaggtcgcc caccgtccac
     1681 ttgtggcgat aggtgaattg cggctgcgtc gcccactcgc gcaggcgcac cagcaactgc
     1741 gtgctctcca ttgcactcat cccgaccaca tgatgcgccg tgcagcccag caccagcgac
     1801 ttgcgccccg aacggtgctt ccacaccagc ggcagttcct gctcgccgat cgccatcatc
     1861 tgcctggtca tggccaggct cggctcgggg tcgtagtaga agagcgacgc ccacgccgag
     1921 tgcaccacgc gcagcgcctc gatctgctgc ttgtcctcct cggccagcat ctcccacgcg
     1981 gcataggtgt tgcagaactc ggtgtcaccg cccgtgggcg acagcacctt ggccgatagc
     2041 agcgaggcca ggatcggcac ctggttcatg gtgccgtcga ggtgccagta cagcgagcct
     2101 ttcaggtact cggcgctcgc gttggccctg gtgtccaggg tgaccttgta gatctcctcg
     2161 ccgcgcattt ccggcgcgaa cgtgccgagc gtgcgggtga acgcgacctg ctcctcgtcc
     2221 gtgaacccga tccgcggaaa gaccagcacg ccgcgacgct cgagcagttc acgcagatcg
     2281 gttgcatgtt cgccgctgag cagcgcgtcc ttgcccagac gaacctcgct ggcaatcagc
     2341 ggagagatgt cgctggcctc gagcttttca ctgatggctg cggtcatggc ttcggtcctt
     2401 caggagtggt gctggaaatc cgggcccttg catcgtgtga cgccgccccg gcgctgcggc
     2461 tggcgactag ctgtccgcca gccggcgtgc ggccagctcg cgcagcggcg ccgtcttcac
     2521 cttgttggaa gcggtcagct ccagatccgc ctcctgcacg aacagcacgc gccgcggcac
     2581 cttgtagctc gacaggcgcg ccgacacgaa ggcacgcacc gtgggctcgt cgagcgtggc
     2641 gccctgttcg cgcacgatac aggtcacgac gatctccccg agcgtctcgt gcgggacgcc
     2701 aaccgtggtg gcgatcttga cgcccgggca ctcgcgcagc agcgcgtcga tttccgtcgg
     2761 cgagacattc gcaccgccgg tcttgatgat gtcgttcagg cgcccgtgcc agtgcagccg
     2821 cccctcggca t
//
LOCUS       DAORUQ010000302         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_54239_length_2717_cov_5.661908, whole genome shotgun sequence.
ACCESSION   DAORUQ010000302 DAORUQ010000000
VERSION     DAORUQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54239_length_2717_cov_5.661908"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PLW13_15800"
     CDS             <1..1253
                     /locus_tag="PLW13_15800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQY71893.1"
                     /translation="AGFDVALVEIGGTVGDIESQPFLEASRQLRGELGTTRALFIHLT
                     LVPYIRSAGETKTKPTQHSVKEMRAMGLQPDILLCRSEVHIDEASRRKIALFTDVQER
                     AVISVPDVDMIYRLPLLLHSEKLDDIVLEKLGLDCPPAKLDEWQAVIDGKLHARRRAT
                     IAMVGKYMDLLDAYKSLNEALTHAGIHTGTRVEMRYIDSEQISREGTGVLEGVDAILV
                     PGGFGERGIEGKIETVRYARENRIPYLGICLGLQVAVIEFARNVAGMEDANSTEFNAA
                     TQHPVVALITEWLKSDGSTERRSQSSDLGGTMRLGSQLCHLEEGSNVRAMYRSAEIME
                     RHRHRYEVNGNFLPRLQEAGLRVAGWSADHSLVEVIEMPGHPWFVACQFHPEFTSTPR
                     DGHPLFSGFVEAAVRYAAEHDRGN"
     gene            1243..2100
                     /gene="kdsA"
                     /locus_tag="PLW13_15805"
     CDS             1243..2100
                     /gene="kdsA"
                     /locus_tag="PLW13_15805"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQY71894.1"
                     /translation="MATEAAARVIGVGAIEVANDRPFVLFGGMNVLESRDLAMQVAEA
                     YVETCAKLAIPYVFKASFDKANRSSIASFRGPGMDEGLKIFQEIKSTFGVPVITDVHE
                     PHQAAPVAEVCDVIQLPAFLSRQTDLVVAMAKTGAAINIKKAQFLAPHEMGHILRKCE
                     EAGNSRLMLCERGSCFGYNNLVVDMLGFGVMKRFGYPVIFDVTHALQMPGGRADSAGG
                     RRQQVVELGKAGMSQGIAGLFLEAHPDPDRAKCDGPCALPLDRLGEFLAQMKAMDELV
                     KSLPALEIR"
     gene            2159..>2717
                     /gene="eno"
                     /locus_tag="PLW13_15810"
     CDS             2159..>2717
                     /gene="eno"
                     /locus_tag="PLW13_15810"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252325.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQY71895.1"
                     /translation="MSAITEVTAIEILDSRGNPTVEASVRLSCGATGTACAPSGASTG
                     SREALELRDGDAARYNGKGVLKAVGAINGEIAGLLRGREAADQRTIDRLMIESDGTEN
                     KSRLGANAILAVSLAVAKAAASAAKLPLYAHIAELNGTPGKYSMPLPMMNIINGGEHA
                     DNNVDIQEFMVQPVGATSFAEALRCG"
BASE COUNT          459 a          909 c          935 g          414 t
ORIGIN      
        1 ccgccggctt cgacgtggcg ctggtcgaga tcggcggcac cgtcggcgac atcgagtcgc
       61 agcccttcct ggaggcgagc cgccagctgc gcggcgaact tggcaccacg cgcgcgctgt
      121 tcatccacct cacgctggtg ccctacatcc gctcggccgg cgagaccaag accaagccca
      181 cgcagcactc cgtgaaggag atgcgcgcca tgggcctgca gccggacatc ctgctgtgcc
      241 gctccgaggt gcacatcgac gaggcctcgc ggcgcaagat cgcgctgttc accgacgtgc
      301 aggagcgcgc ggtgatctcg gtgcccgacg tcgacatgat ctaccgcctg ccgctgctgc
      361 tgcacagcga gaagctcgac gacatcgtgc tcgagaagct cggcctcgat tgtccgccgg
      421 cgaagctcga cgagtggcag gccgtgatcg acggcaagct gcacgcgcgg cgccgcgcga
      481 cgatcgcgat ggtcggcaag tacatggacc tgctcgatgc ctacaagtcg ctgaacgagg
      541 cgctgacgca cgccggcatc cacaccggca cgcgggtcga gatgcgctac atcgactccg
      601 agcagatctc gcgcgagggc accggcgtgc tcgagggcgt ggacgcgatc ctggtgccgg
      661 gcggtttcgg cgagcgcggc atcgagggca agatagagac cgtgcgctat gcgcgcgaga
      721 accgcattcc ctacctcggc atctgcctcg gcctgcaggt ggcggtgatc gagttcgcgc
      781 gcaacgtcgc cggcatggag gatgcgaaca gcaccgagtt caacgccgcc acgcagcacc
      841 cggtggtcgc gctgatcacc gagtggctga agtccgacgg cagcaccgag cgtcgcagcc
      901 agtcctcgga cctcggcggt acgatgcgac tcggctcgca actgtgccac ctcgaggagg
      961 gctcgaacgt gcgcgcgatg taccgcagcg ccgagatcat ggagcgccac cgtcaccgct
     1021 acgaagtcaa cggcaatttc ctgccgcgcc tgcaggaggc ggggctgcgc gtggcgggct
     1081 ggtcggccga tcattcgctg gtcgaggtga tcgagatgcc cggtcacccg tggttcgtgg
     1141 cctgccagtt ccaccccgag ttcacctcga cgccgcgcga cggccatccg ctgttcagcg
     1201 gtttcgtcga ggccgccgtg cgctatgccg cggagcacga ccgtggcaac tgaggccgcg
     1261 gccagggtca tcggcgtcgg cgcgatcgag gtcgccaacg accggccgtt cgtgctcttt
     1321 ggcggcatga acgtgctcga gtcgcgcgac ctcgcgatgc aggtcgccga ggcctacgtc
     1381 gagacctgcg cgaagctcgc gatcccctac gtgttcaagg cttccttcga caaggccaac
     1441 cgctcctcga tcgcctcgtt ccgcggcccc ggcatggacg aggggctgaa gatcttccag
     1501 gagatcaaga gcaccttcgg cgtgccggtg atcaccgacg tccacgagcc gcaccaggcc
     1561 gcgccggtgg cggaagtctg cgacgtgatc cagctgccgg cgttcctgtc gcgccagacc
     1621 gatctcgtgg tcgcgatggc gaagacgggc gcggcgatca acatcaagaa ggcgcagttc
     1681 ctggcgccgc acgagatggg ccatatcctg cgcaagtgcg aggaggcggg caattcgcgc
     1741 ctgatgctgt gcgagcgtgg cagttgcttc ggctacaaca acctcgtggt cgacatgctc
     1801 ggcttcggcg tcatgaagcg cttcggttac ccggtgatct tcgatgtcac gcatgcgctg
     1861 cagatgccgg gcgggcgcgc cgactccgcg ggcggtcgcc gccagcaggt ggtcgagctc
     1921 ggcaaggccg gcatgtcgca gggcatcgcg gggcttttcc tcgaggccca tcccgatccc
     1981 gaccgggcga agtgcgatgg cccctgcgcg ctgccgctgg atcgcctcgg cgagttcctg
     2041 gcgcagatga aggccatgga cgaactggtg aagagcctgc cggcgctcga gatccgctga
     2101 ggcacgcgcc ccgggcgaca acaacgaatc gataatcgct acaggagatg gattggtcat
     2161 gtctgcaatc accgaagtga ctgcgatcga gattctggat tcgcgcggca accccacggt
     2221 ggaggccagc gtgcgtcttt cctgcggcgc gacgggcacc gcctgcgcgc cctcgggggc
     2281 ctcgacgggc tcgcgcgagg cgctggaact gcgcgatggc gatgccgcgc gctataacgg
     2341 caagggagtg ctgaaggccg tgggcgcgat caacggcgag atcgcggggc tgctgcgcgg
     2401 gcgcgaagcc gccgaccagc gcacgatcga ccggctgatg atcgaatccg acggcaccga
     2461 gaacaagtcg cgcctcggcg ccaacgcgat cctcgcggtg tcgctggccg tcgcgaaggc
     2521 tgcggccagc gcggcgaaac tgccgctcta cgcgcacatc gccgagctca acggcacgcc
     2581 gggcaagtac tcgatgccgc tgccgatgat gaacatcatc aacggcggcg agcatgccga
     2641 caacaacgtc gacatccagg agttcatggt gcagccggtc ggcgcgacca gcttcgccga
     2701 agcgctgcgc tgcggcg
//
LOCUS       DAORUQ010000303         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_54424_length_2711_cov_6.982304, whole genome shotgun sequence.
ACCESSION   DAORUQ010000303 DAORUQ010000000
VERSION     DAORUQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54424_length_2711_cov_6.982304"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLW13_15815"
     CDS             <1..402
                     /locus_tag="PLW13_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQY71896.1"
                     /translation="LQAEGRRVAMAGDGINDAPALAAADVGIAMGTGTDVAMSSAQVT
                     LVKGDLQGIVRARAISRAAVANMKQNLAFAFLYNALGVPVAAGVLYPAFGLLLSPMIA
                     ALAMSLSSVSVVSNALRLRRAPAGKTMETRQ"
     gene            399..1898
                     /locus_tag="PLW13_15820"
     CDS             399..1898
                     /locus_tag="PLW13_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQY71897.1"
                     /translation="MSRRLATAVLVLGATLAGCAPLPTDDGRSATAELLAARGQQPPT
                     AGDTVASLLARLREQPLSAADAVRLALLNNPDMKAEYAGLGFAAADVYEAGRMANPLL
                     SASVLFPDTGGEANQVGFGLAQNFTDVLLLRSRSRLAEGEYARARELIGAKALALAAD
                     TEAAHLRVVGSWQLIAMREAVAQAATVSAELAARFHAAGNIGRLELALQQAAASEAKL
                     DVLQARAELHAARSALNRLMGLAPTEDRWRVVDRLPVPLAADDAVADLLRHADASRLD
                     LAAARREVALLAEALGVTQSFRLLGDVELGVTTERETDRSRLTGPTLGLELPLFDHGH
                     GRVARAQASLQRAQAQLRALEIDIGNAVQRGAAEVAAAKARADHYRESLIPLREEIVA
                     RTQREVNFMLADQFQLLFVKQQEYDAYQGYLEAVRDYWLARVELGRAVGAALPSSARV
                     GEAALDAQTLTQPESRPAPMDHSQHMQHDASAAPPPPHATHESSHGDRP"
     gene            1895..>2711
                     /locus_tag="PLW13_15825"
     CDS             1895..>2711
                     /locus_tag="PLW13_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQY71898.1"
                     /translation="MISRRNILSRIGLGSLATLLVAVAQRSRAQGAHEGHAMPAATAS
                     APAGTAATGSARPGASFPAAGYTPVRTLNGWTLPYTLKDGVKEFHLVAEEVEHEFAPG
                     CVAKCWGYNGSTPGPTLEAVEGDRVRIFVTNRLPEHTTIHWHGLYLPSGMDGVGGLNQ
                     PQIQPGETWAYEFTLRQHGTCLYHPHADEMTQLAFGMMGFFIIHPRESQEPPVQRDYA
                     LLLHNWALHPGTYRPDPAVMTEFDLWTFNSKVFPATEPLLAAQGERVRLRVGNL"
BASE COUNT          406 a          989 c          955 g          361 t
ORIGIN      
        1 ctccaggccg aggggcggcg cgtcgcgatg gccggcgacg gcatcaacga tgcgccggcg
       61 ctcgccgcgg ccgacgtcgg cattgccatg ggtaccggca ccgacgtggc gatgtcgagc
      121 gcccaggtga cgctggtcaa gggtgacctg cagggcatcg tccgcgcccg cgcgatctcg
      181 cgcgcggcgg tcgcgaacat gaagcagaac ctcgcgttcg cgttcctcta caacgcgctt
      241 ggcgtgccgg tggccgcggg ggtgctgtac ccggctttcg gcctgctgtt gagcccgatg
      301 atcgcggcgc tggccatgag cctgtcctcg gtctcggtgg tgagcaacgc gctgcggctg
      361 cgccgcgcac ccgctggcaa aaccatggag acccgtcaat gagtcgacgt ctggccaccg
      421 ccgtactggt gttgggcgcc acgctggccg gctgcgcgcc cctgcccacg gatgacgggc
      481 gcagcgcaac ggcggagctg ttggccgcgc gcggccagca accgcccacc gccggcgaca
      541 ccgtcgcctc gctgctggcg cggttgcgcg agcagccgct gagcgcggcc gacgcggtcc
      601 gcctggcgct gctgaacaac cccgacatga aagccgaata cgccggcctg ggcttcgccg
      661 cagccgatgt ctacgaggcc gggcgcatgg ccaatccgct gctgtccgcc tcggtgctgt
      721 ttcccgatac cggcggcgag gccaaccagg tcggcttcgg cctcgcccag aacttcaccg
      781 acgtgctgct gctgcgatcg cgcagccgcc tggccgaggg cgagtacgca cgcgccaggg
      841 agctgatcgg cgcgaaggca ctcgcgctgg cggcggatac cgaggccgcc cacctgcgcg
      901 tcgtcggcag ctggcagctg atcgcgatgc gcgaagcggt cgcgcaggcg gcgacggtct
      961 cggccgaact ggcggcacgg ttccacgcgg cgggcaatat cggccgtctc gaactggcac
     1021 tgcagcaggc cgccgcctcg gaggcaaagc tcgacgtgct gcaggcgcgc gccgagctgc
     1081 acgcggcgcg cagcgcactg aaccggctga tgggcctggc cccgaccgag gaccgctggc
     1141 gcgtcgtgga ccgcctgccc gtcccgctcg ccgcggacga cgccgtggcg gacctgctgc
     1201 gacacgccga tgccagccgc ctcgacctgg cggcggcgcg gcgcgaagtc gcgctgctcg
     1261 ccgaggcgct cggcgtgacg cagagcttcc ggctgctggg cgatgtcgag ctaggcgtga
     1321 caaccgagcg cgagaccgac cgctcccgtc tcaccggacc gacgctgggt ctggagctgc
     1381 cgctgttcga ccacggccac gggcgcgtgg cgcgcgcgca ggcgagcctg caacgggcgc
     1441 aggcgcaact gcgcgcgctg gagatcgaca tcggcaacgc cgtgcagcgc ggcgccgccg
     1501 aggtcgccgc cgcgaaggcg cgcgccgatc actaccgcga gtcgctgatc ccgctgcgcg
     1561 aggagatcgt cgcgcgcacg cagcgcgagg tgaatttcat gctcgccgac cagttccagc
     1621 tgttgttcgt gaaacagcag gaatacgacg cctaccaggg ctacctcgag gcggtacgcg
     1681 attactggct ggcacgcgtg gagctcgggc gcgcggtcgg cgcggcgctg ccgagcagcg
     1741 cgcgcgtggg cgaggccgcg ctcgatgcgc aaacgctgac gcagcctgag agtcggcccg
     1801 cgcccatgga tcactcgcag catatgcagc acgacgcctc ggcggcgccg cccccgccgc
     1861 acgccaccca cgaatccagc cacggagacc ggccatgatc agccgccgca acatcctgtc
     1921 gcgcatcggc ctgggctcgc tcgcgacact gctcgtagcc gtcgcgcagc gcagccgcgc
     1981 gcagggcgcg cacgagggcc acgccatgcc cgcagcaacg gcatccgcgc cagccgggac
     2041 cgccgcgacc ggcagcgcgc ggcccggcgc gtctttcccc gccgcgggct acacgccggt
     2101 gcgcaccctg aacggctgga ccctgccgta cacgctgaag gacggcgtga aggagttcca
     2161 cctggtggcc gaggaagtgg agcacgagtt cgcccccggc tgcgtcgcga agtgctgggg
     2221 ctacaacggc agcaccccgg gcccgacgct cgaggcggtg gaaggcgacc gcgtgcgcat
     2281 cttcgtgacc aaccgcctgc ccgagcacac cacgatccac tggcacggcc tgtacctgcc
     2341 gagcggcatg gacggcgtgg gcggcctgaa ccagccgcag atccagcccg gggaaacctg
     2401 ggcatacgag ttcacgctgc gccagcatgg cacctgcctg tatcacccgc atgcggacga
     2461 gatgacgcaa ctcgccttcg gcatgatggg tttcttcatc attcatccgc gggaatcgca
     2521 ggagccaccg gtgcagcgcg actacgcgct gctgctgcac aactgggcgc tgcaccccgg
     2581 cacgtatcgc cccgatcctg cggtgatgac cgagttcgac ctgtggacct tcaacagcaa
     2641 ggtgttcccc gccaccgaac cgctgcttgc ggcgcagggc gagcgcgtgc gccttcgcgt
     2701 cggcaacctt t
//
LOCUS       DAORUQ010000304         2702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_54713_length_2702_cov_4.123158, whole genome shotgun sequence.
ACCESSION   DAORUQ010000304 DAORUQ010000000
VERSION     DAORUQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54713_length_2702_cov_4.123158"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..873)
                     /locus_tag="PLW13_15830"
     CDS             complement(202..873)
                     /locus_tag="PLW13_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY71899.1"
                     /translation="MPAPARPARGRPLLSHDRIVDTALELLHGLALKELTMRRLAQAL
                     DTTPAALYGYFRSQEELFAAVATRVVRGIELGALEEVADWRERLRLWARAVRSRQLEF
                     ASTVAMAQISPHIPASWFEVTAPLLRALEMAGLSGKALIDTARCFSRIVYGSILTELA
                     QDPYLLQCERAEAGAALEHLSPSAAGQVAAILPYLGAQDNEALFEMTIDCALRGIAAG
                     LPPLA"
     gene            1000..>2702
                     /locus_tag="PLW13_15835"
     CDS             1000..>2702
                     /locus_tag="PLW13_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71900.1"
                     /translation="MQEFDIVIRNGMIVDGARNPRWKGDIGIRDGVITRMGKIANPKA
                     RRVIDADGLVVAPGFIDLHTHYDAQVFWDPYLSTSGFHGVTSVVLGNCGFGFAPMAPE
                     LRDCAMKSMTKVEAIPLVTLQTALPWDWTTYPEYLDSMDRAPKSLNVLPYVGLNPLMI
                     AAMGFEDAKSGRMPTEAEHAEMARLLNEAMDAGACGFSAQCLGTPGKPEAGSVGGAPQ
                     NDFDGTPFPTDVMWPETRMHMAKVLGERGEGFIALSMGMTPPPEWEALAAASGAPILW
                     QAVPPSGDEMGTGIRRHLFDWLTSCHERGLRIYGQGITQDPPLIFTFDYFDLWSDWNA
                     FVGPDVPREQKLANFADPGVREKLRALPLKYMFIDAIPDTRLHRTFSEGNKPFEGMRL
                     SEIAEKTGRHVVDVVCDIVLADGLKTIFETRQFDTTLAGVKELLDNPYIIPGLSDGGA
                     HLKYLTAGSYGTELIADYVRTHQLISLEEAHWRLSSLPAFCAGFRDRGTLREGAAADI
                     IVYDYEKLAFTFPEFVQDLPGDEYRVTNRGSGYRYVLVNGELTIEDDKPTNVHSGALL
                     R"
BASE COUNT          459 a          925 c          901 g          417 t
ORIGIN      
        1 gaattcctcg agcttttgcg ccttgctgcg gatgcgcgcc tccagcgctt ccgaatgctc
       61 catgtcgcga accgtgatct gcagtggtat ttgcatggat ccatccttct tcttgaggtc
      121 aggcatttca ggataacgta tcggggacgc atcggacacg ccacatggcg gggcgatggc
      181 gcaaatcaac gaacgggacg ctcaggcgag cggcggcagc cccgcggcga tgccgcgcaa
      241 cgcgcagtcg atggtcatct cgaacagcgc ctcgttgtcc tgcgccccca ggtacggcag
      301 gatcgccgcg acctgcccgg cagcggacgg cgacaggtgc tcgagggcgg cgccggcctc
      361 ggcgcgctcg cactgcagca ggtagggatc ctgcgcgagc tcggtgagta tcgagccgta
      421 gacgatgcgc gagaaacagc gcgcggtgtc gatcagggcc ttgccggaca ggccggccat
      481 ctccagcgcg cgcagcagcg gggccgtgac ctcgaaccag ctggcgggaa tgtgggggct
      541 gatctgcgcc atcgcgaccg tgctcgcgaa ctcgagctgg cgcgagcgca ctgcgcgcgc
      601 ccacagccgc agccgctcgc gccagtccgc cacctcctcc agggcgccca gctcgatgcc
      661 gcgcacgacg cgcgtggcca cggcggcgaa cagttcctcc tggctgcgga aatagccgta
      721 tagcgccgcc ggcgtcgtgt ccagcgcctg cgccagccgc cgcatcgtca gttccttcag
      781 cgccagcccg tgcagcagtt ccagcgccgt gtccacgatc cggtcgtgcg agagcagcgg
      841 gcgtccgcgg gcgggacggg cgggtgcggg catcgcgctt ctccgtggct gggggcggaa
      901 gtgctcgtcg gccggcgtgc ttgacggccc gacgcgacgc agtttaacct gacggcgttt
      961 acttaacact gttcacaaat aattgcgaag gagggtctcg tgcaggaatt cgacatcgtc
     1021 atcaggaacg gcatgatcgt cgacggcgcg cgcaacccgc gctggaaggg cgacatcggc
     1081 atcagggacg gcgtgatcac gcgcatgggc aagatcgcca acccgaaggc caggcgcgtg
     1141 atcgacgctg acggactggt ggtcgcgccc ggcttcatcg acctgcacac gcactacgac
     1201 gcacaggtgt tctgggatcc ctacctctcc acctcgggct ttcacggcgt gacctcggtg
     1261 gtgctgggca actgtggctt cggcttcgcg ccgatggcgc ccgaactgcg cgactgcgcc
     1321 atgaagtcga tgaccaaggt ggaggccata cccctggtga cgctgcagac cgcgctgccc
     1381 tgggattgga ccacctaccc cgagtacctc gacagcatgg accgcgcgcc caagagcctg
     1441 aacgtgctgc cctacgtggg ccttaatccg ctgatgatcg ccgcgatggg cttcgaggat
     1501 gccaagtccg gacgcatgcc gaccgaggcc gagcacgccg agatggcccg actgctcaac
     1561 gaggcgatgg atgccggcgc ctgcggcttc tccgcgcagt gcctgggcac ccccggcaag
     1621 ccggaggccg gcagcgtcgg cggcgcgcca cagaacgatt tcgacggcac gcccttcccc
     1681 accgacgtga tgtggcccga gacgcgcatg cacatggcca aggtgctggg cgagcgcggc
     1741 gagggtttca tcgcgctgtc gatggggatg acgccgccgc ccgagtggga agcgctggcc
     1801 gcggcgagcg gcgcgccgat cctgtggcag gccgtgccgc cgtccggcga cgagatgggc
     1861 accgggatcc gccgccacct gttcgactgg ttgacgagtt gtcacgaacg cggcctcagg
     1921 atctacggcc agggcatcac gcaggatccg ccgctgatct tcaccttcga ctacttcgac
     1981 ctgtggagcg actggaacgc cttcgtcggc cccgacgtgc cgcgcgaaca gaagctcgcg
     2041 aacttcgccg atccgggcgt gcgcgagaag ctgcgcgccc tgccgctgaa gtacatgttc
     2101 atcgacgcga tcccggacac ccgcctgcac cgcaccttca gcgagggcaa caagcccttc
     2161 gagggcatgc gcctcagcga gatcgcagaa aagaccgggc ggcacgtggt cgacgtggtc
     2221 tgcgacatcg tgctggccga cgggctgaag acgatcttcg agacgcgcca gttcgacacc
     2281 acgctcgcgg gcgtcaagga actgctcgac aacccctaca tcatcccggg gctctccgac
     2341 ggtggcgccc acctgaagta cctcaccgca ggcagctacg gcaccgagct gatcgcggac
     2401 tacgtgcgca cgcaccagct gatctcgctc gaggaagcgc actggcgcct gagctcgctt
     2461 cccgccttct gcgccggttt ccgcgatcgc ggcacgctgc gcgagggcgc ggccgccgac
     2521 atcatcgtct acgactacga gaagctcgcc ttcaccttcc cggagttcgt gcaggatctg
     2581 cccggcgacg agtaccgcgt gaccaaccgc ggcagtggct accgctacgt gctggtcaac
     2641 ggcgagctca cgatcgagga cgacaagccc acgaacgtgc actcgggcgc gctgctgcgc
     2701 aa
//
LOCUS       DAORUQ010000305         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_55322_length_2684_cov_5.755801, whole genome shotgun sequence.
ACCESSION   DAORUQ010000305 DAORUQ010000000
VERSION     DAORUQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55322_length_2684_cov_5.755801"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLW13_15840"
     CDS             complement(<1..468)
                     /locus_tag="PLW13_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQY71901.1"
                     /translation="MIVKDNTMSARPRKVLIFGLDGPVAPALLRYCREGKLPALARLI
                     DNGVLAPNAMVPLPTATPANWTSIATGAWPGTHGISDYNVRRPGDPLDRAHMAFHSPD
                     VKAEYLWNAIARAGKKSVVVNFVTTWPPVVDDGIQIGGSGCDINQWFHPNLALA"
     gene            549..1031
                     /locus_tag="PLW13_15845"
     CDS             549..1031
                     /locus_tag="PLW13_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQY71902.1"
                     /translation="MVDQQELQRGADRVAIQDLMARYAYAVDAKRWELLDEVFVEGAE
                     IDFLANGGVKDNWPAIGGWLATAMSGFTACQHYLSNFATEVDGDRASSRFYVFTQMVT
                     VQDGSDHIDSDGGYYNAAFVRTPAGWRVSRLSGGIVWWSGSVPDHLPWWGVDGDRFNQ
                     "
     gene            complement(1041..1793)
                     /locus_tag="PLW13_15850"
     CDS             complement(1041..1793)
                     /locus_tag="PLW13_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71903.1"
                     /translation="MRFRDQVAVVTGGASGLGRATARRFAAEGARVALLDRDATAGEE
                     SAAEIRAAGGTASFHAVDVCSWASIEPAIATVSAELGAPGVLVNCAGIGNFVRSELET
                     QHNWERTIAVNLTGTFLMCRSVLPAMLAAGRGSIVNIASNSGLLGQPYAAAYCASKGG
                     IINLTRALAVEYRARGVRVNAIAPGGMHTPMLSSWALPEGADIAEFATGTSPLGYAEP
                     AEIAGLIAFVASDEGRYMVGQIVGIDGGITCG"
     gene            1946..2623
                     /locus_tag="PLW13_15855"
     CDS             1946..2623
                     /locus_tag="PLW13_15855"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQY71904.1"
                     /translation="MKTHTLESQSSMTPRNALQILKEGNQRFVNNLKASRNLLQQVNE
                     TREGQWPFAVILSCIDSRTSAELIFDQGLGDVFSIRIAGNIVNTDIIGSIEFACKVAG
                     SKLIVVMGHSKCGAIKGACDHVEMGNLTELLAKIQPAVYQETQTVEKEHRNSANDVFV
                     ENVASLNVKRSVRAIVERSFILEQMIEKGEIGIIGAKHDLETGAVEFYGESLLCNQAD
                     VQGTLGA"
BASE COUNT          489 a          848 c          902 g          445 t
ORIGIN      
        1 ggcgagcgcg aggttcgggt ggaaccactg gttgatgtcg cagcccgagc cgccgatctg
       61 gatgccatcg tccacgaccg gcggccaggt ggtgacgaag ttcaccacga ccgacttctt
      121 gccggcacgc gcaatggcat tccagagata ttcggccttc acgtcggggc tgtgaaatgc
      181 catgtgggcg cgatccagcg ggtcgccggg acgacgcacg ttgtagtcgg atatgccgtg
      241 cgtgccgggc catgcgccgg tggcgatgga ggtccagttg gcgggtgtcg cggtcggcag
      301 cgggaccatg gcattgggtg ccagcacgcc gttgtcgatc agccgcgcga gcgcgggcag
      361 cttgccttcg cggcagtagc gcagcagcgc cggcgcgacc ggaccgtcga ggccgaagat
      421 aagtaccttg cgtggtcgtg cactcatggt gttgtctttg actatcatgg tgatgctcgt
      481 gacactgcgg tcgagcataa atgataaccg ggtgcgaatg acacgtgccc tgccgggagg
      541 aagcgcggat ggtcgatcag caggagctgc agaggggtgc cgaccgcgtc gccatccagg
      601 atctcatggc gcgttatgca tacgcggtcg atgccaagcg ctgggaactg ctggacgagg
      661 tcttcgtgga gggtgcggag atcgatttcc tggccaatgg cggggtcaag gacaactggc
      721 ccgcgatcgg cggctggctc gcgacggcca tgtcgggatt cacggcctgc cagcattacc
      781 tctccaattt cgccaccgag gtcgacggtg atcgcgcgag ctcgcgcttc tacgtgttca
      841 cgcagatggt cacggtgcag gacggctccg atcatatcga cagcgacggc ggctactaca
      901 acgcggcatt cgtgcgcaca ccggcgggct ggcgcgtcag ccggctttcc ggcggcatcg
      961 tgtggtggag cgggtcggtg cccgatcacc tgccgtggtg gggcgtggac ggggatcgct
     1021 tcaaccagta ggcgcggcgc tcaaccgcag gtgatgccgc cgtcgatacc gacgatctgg
     1081 ccgaccatgt agcgtccctc gtccgatgcg acgaatgcga tcagtcccgc gatttccgcc
     1141 ggctcggcgt agccgagcgg cgaggtgccc gtcgcgaatt ccgcgatatc cgcgccctcg
     1201 ggaagcgccc acgaggaaag catcggcgtg tgcatgccgc cgggtgcgat ggcattcacc
     1261 cgcacgccgc gggcgcggta ttccaccgcc agcgcgcgcg tgagattgat gatcccgccc
     1321 ttcgatgcgc aatacgccgc cgcatagggc tggcccagaa gccccgagtt ggacgctatg
     1381 ttcacgatgg acccgcgccc cgcggcgagc atcgccggca ggacgctgcg gcacatcagg
     1441 aaggtgccgg tcaggttcac cgcgatggtg cgctcccagt tgtgctgggt ctcgagttcg
     1501 ctgcgcacga agttgccgat gccggcgcaa ttgaccagca cgccgggggc gccgagctcg
     1561 gcgctcaccg tcgcgatggc gggctcgatc gatgcccagc tgcacacgtc gaccgcatgg
     1621 aaactggccg tgccgcccgc cgcgcggatc tcagccgccg actcttctcc cgccgtggcg
     1681 tcgcggtcga ggagggcaac gcgcgccccc tctgcggcga agcggcgcgc agtcgcgcgt
     1741 ccgagccccg aagcgccgcc ggtgaccacc gcgacctgat ccctgaatcg caccattgct
     1801 gcttgccccc gtatcgatcc acgcgctgag cgccgcgcag cacgagccta tcagctcgtg
     1861 cggatgtggc caacgcaagg cgcccgtcga tcaggcgcgc cggtatactg gcctgccaag
     1921 gaaccgctcg ccacgcaggg tagccatgaa gacgcacacg cttgaatcac agagttcgat
     1981 gacgccgcga aacgccctgc agatcctgaa ggaaggcaac cagcgcttcg tcaacaacct
     2041 gaaagccagc cgcaacctgc tgcagcaggt caatgaaacc cgtgaaggcc agtggccatt
     2101 cgcggtgata ctgagttgca tcgacagccg cacctcggcc gagctgatct tcgaccaggg
     2161 cctgggcgac gtgttctcga tccgcatcgc cggcaacatc gtcaataccg acatcatcgg
     2221 cagcatcgaa ttcgcctgca aggtcgccgg ttccaagctc atcgtggtga tgggccattc
     2281 caagtgcggc gcgatcaagg gcgcctgcga tcacgtcgaa atggggaacc tgaccgagct
     2341 gctcgcgaag atacaacctg ccgtttacca ggagacacag acggtcgaaa aagaacacag
     2401 gaattcggcc aatgacgtct tcgtcgagaa tgtcgcgagc ttgaacgtga agcgatctgt
     2461 cagggccatt gtcgagcgca gcttcatcct cgagcagatg atcgaaaagg gcgagatcgg
     2521 aatcatcggc gccaagcacg atctcgaaac cggcgcggtc gagttctacg gcgagtcgct
     2581 gctctgcaac caggcggatg tgcagggcac gctgggcgcc tgagggcggg ccgcgcaggt
     2641 atccagaacc aggccgcgca gccattgatg ggccggatcg gcat
//
LOCUS       DAORUQ010000306         2680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_55478_length_2680_cov_7.063238, whole genome shotgun sequence.
ACCESSION   DAORUQ010000306 DAORUQ010000000
VERSION     DAORUQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55478_length_2680_cov_7.063238"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2679)
                     /gene="glgP"
                     /locus_tag="PLW13_15860"
     CDS             complement(133..2679)
                     /gene="glgP"
                     /locus_tag="PLW13_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQY71905.1"
                     /translation="MAGSRFTLEVQPRIPAPLARLDELANNLVYSWDRRVRGLFARLD
                     RALWAACGSNPRVFLRRVAQAVLDEAAEDRGYLEEYRRVLSSFDAYMERDPRPECQQF
                     LDPDDDLVAYFCAEFGLHESFPIYSGGLGILAGDHCKAASDLGIPFVGVGLLYHNGYF
                     TQTIDGHGNQISQIVPSDFRDMPIQPVNLDGERLRIDLDFPGRSVGVHVWEARIGHIR
                     LYLLDTDVAENSEADRRITRQLYGGGREMRLEQEIVLGIGGTRVLRRLGLKPTAWHIN
                     EGHAAFLVLERCREYVQQGHTLASALEIVAAGTVFTTHTPVPAGHDIFDTPLFEAYFR
                     GFAESLGVPMHELLALGSVPEPNGSFNMTTLALKGSRFHNGVSRIHGEVASVMERAVW
                     PQIPPEENPMTHVTNGVHLKTFLASEWYNLFDMRFDEWHDELLNPDYWDRIEQIPDYQ
                     FWSVRKQLKEWLLQELHHRVVQQYRRNGESEATIARVTHLIAKPDADTLVMGFARRFA
                     TYKRATLLFSDPARLARLLNDPERPTVIVFAGKAHPSDAPGQHLIKVIHEFSQRPEFQ
                     GRIIVLEGYDIRLARQLVSGVDLWLNTPEYPLEASGTSGQKAAMNGVVNLSVLDGWWG
                     EGFNGKNGWGIVPREPRLDIEQRNRDEAKDLLDLIEHEVLPLYFARNGHGFSSGWVAL
                     AKASMKSIIPRFNSLRMVMDYVTRLYCPAREQHRRLSADDGAGARELAQWKARVRSRW
                     PGVRITLENEPPGAVHHDEAVQLRVRVALNGLQASDVVVECLVDPAPESEGASAQRVQ
                     LEPLTDSGEERIFAADFHALNSGLQEMRLRIYPHNALLSHRFELGLMLWL"
BASE COUNT          410 a          871 c          949 g          450 t
ORIGIN      
        1 tcgcgatgta tcgcgccgcg ccgtaggtcc ggctgtagcc ggacaatgtc cggataaatc
       61 cggacctacg tgccggcgat gtccagatga acccggaccc acgtgtgggg cgatgtccgg
      121 atgaatccgg atctacagcc agagcatgag cccgagctcg aagcggtggc tgagcagcgc
      181 gttgtgcggg tagatgcgca gacgcatctc ctgcaggccg gaattcagcg cgtggaaatc
      241 ggccgcgaaa atccgctctt cgccgctgtc ggtcagcggt tccagttgca cgcgctgcgc
      301 gctggcaccc tcggattccg gtgccggatc caccaggcat tcgacaacca cgtccgaggc
      361 ctgcaggccg ttcagtgcca cgcgcacgcg caactgcacc gcctcgtcgt ggtgtaccgc
      421 gccgggcggc tcgttctcca gcgtgatgcg cacgccgggc cagcgcgagc gcacgcgcgc
      481 cttccactgc gcgagctcgc gcgcgccggc gccgtcgtcg gcgctcaggc gccggtgctg
      541 ttcgcgcgcc gggcagtaca gccgcgtcac gtagtccatc accatgcgca gcgagttgaa
      601 gcgcgggatg atggatttca tcgaggcctt ggccagcgcg acccatcccg aggaaaagcc
      661 gtggccgttg cgggcgaagt agagcggcag cacctcgtgc tcgatcaggt ccagcaggtc
      721 cttggcctcg tcgcggttgc gctgctcgat gtcgagccgc ggctcgcgcg gcacgatgcc
      781 ccagccgttc ttgccgttga aaccctcgcc ccaccagccg tcgagcacgc tcaggttcac
      841 cacgccgttc atcgcggcct tctggcccga ggtcccgctc gcctcgagcg ggtattccgg
      901 cgtgttcagc cacaggtcca cgccggagac cagttgccgc gcgaggcgga tgtcataacc
      961 ctcgagcacg atgatgcgcc cctggaactc ggggcgctgg gagaactcgt ggatcacctt
     1021 gatcaggtgc tgtcccggcg cgtcgctggg gtgcgccttg ccggcgaaca cgatcacggt
     1081 ggggcgctcg gggtcgttca gcagccgcgc gagtcgcgcc gggtcggaga acagcagcgt
     1141 ggcgcgcttg taggtggcga agcgccgcgc gaagcccatg accagcgtgt cggcgtcggg
     1201 tttggcgatg aggtgcgtga cgcgcgcgat cgtggcctcg ctctcgccgt tgcgccggta
     1261 ctgctgcacc acgcggtggt gcagttcctg cagcagccat tccttgagct gcttgcgcac
     1321 gctccagaac tggtagtcgg ggatctgctc gatgcgatcc cagtaatcgg gattgagcag
     1381 ttcgtcgtgc cactcgtcga agcgcatgtc gaacaggttg taccactcgc tggcgagaaa
     1441 cgtcttgagg tgcacgccgt tggtgacgtg cgtcatcggg ttctcctcgg gcggtatctg
     1501 cggccacacc gcgcgttcca tgaccgaggc cacctcgccg tggatgcgcg agacgccgtt
     1561 gtggaaacgc gagcccttca gtgccagcgt ggtcatgttg aagctgccgt tgggctccgg
     1621 gacgctgccg agcgcgagca gttcgtgcat cgggacaccg aggctctcgg cgaatccgcg
     1681 gaagtaggcc tcgaacagcg gggtatcgaa gatgtcgtgt ccggcaggca ccggggtgtg
     1741 cgtggtgaac acggtgcccg cggcgacgat ctccagcgcg cttgccagcg tgtggccctg
     1801 ctgcacgtac tcgcggcagc gctcgagcac caggaaggcc gcgtggccct cgttgatgtg
     1861 ccatgcggtg ggcttcaggc cgaggcggcg cagcacgcgc gtgccgccga tgccgagcac
     1921 gatctcctgc tccaggcgca tctcgcgacc gccgccgtag agctggcgcg tgatgcgacg
     1981 gtccgcctcg ctgttctcgg cgacatcggt gtcgagcagg tagaggcgga tatggccgat
     2041 gcgcgcctcc cagacgtgca cgccgacgct gcgtcccggg aaatcgaggt cgatgcgcag
     2101 ccgctcgccg tcgaggttca ccggctggat cggcatgtcg cggaaatccg agggcacgat
     2161 ctgcgagatc tggttgccgt ggccgtcgat ggtctgggtg aagtagccgt tgtgatacag
     2221 caggccgacg ccgacgaagg ggatgccgag gtcgctggca gccttgcagt ggtcgccggc
     2281 gaggatgccg aggccgccgg agtagatcgg gaagctctcg tgcagcccga actcggcgca
     2341 gaagtatgcg acgaggtcgt cgtccggatc caggaactgc tggcactcgg gccgagggtc
     2401 gcgttccatg taggcgtcga agctgctcag cacgcgccgg tattcctcga ggtagccgcg
     2461 gtcctcggcg gcctcgtcga ggacggcctg cgccacgcgg cgcaggaaca cgcgcgggtt
     2521 ggagccgcac gcggcccaca gcgcgcggtc caggcgcgcg aacaggccac gcacgcgccg
     2581 gtcccagctg tagaccaggt tgttcgcgag ctcgtcgagc cgcgccagcg gcgccgggat
     2641 gcggggctgg acttccaggg tgaaacgact gcctgccatg
//
LOCUS       DAORUQ010000307         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_55894_length_2668_cov_4.468427, whole genome shotgun sequence.
ACCESSION   DAORUQ010000307 DAORUQ010000000
VERSION     DAORUQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55894_length_2668_cov_4.468427"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..>2668
                     /locus_tag="PLW13_15865"
     CDS             342..>2668
                     /locus_tag="PLW13_15865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY71906.1"
                     /translation="MLKLSFGLDFADLHQREGLARLDAQFLHFIATPDPALREALLAA
                     RAAPEALAPKAESELLIALAPQLEDFVAQLFGIEAEARALAQQHHELAPVFSVKRLFV
                     QRQALAKFKPEDVIDLDADATEQELLGGPFSELAFARRITAWQEDEAANAGQLELAAR
                     YAAWAAGTEAGRARHRDGVLFKAPAKLDYQNLVPLITRETLGFPRHTIDHLRRREGFA
                     LTDHGTDLAGALDEANYCIWCHNQGKDSCSRGLREKAPRGADPASAPFRKSPFGVTLA
                     GCPLEEKISEFHLVKTQGQPIAALAMIVVDNPMAAATGHRICNDCMKSCIYQKQQPVD
                     IPQAETRTLKDVLELPWGFEIYALLTRWNPLNLRRPLPLADTGRKVLVVGLGPAGFTL
                     AHHLMNDGHTVVGIDGLKIEPLPAELSGVDLRGARVRFEPVRDVKSLMEPLDARVMAG
                     FGGVAEYGITVRWDKNFLKLIRLLVERRSAFAMFGGVRFGGTVTPEDAWRMGFDHIAL
                     CVGAGGPTVLDIPNGLARGVRAASDFLMALQLTGAAKPDSIANMQLRLPVVVIGGGLT
                     AIDTATESLAYYVVQVEKFLARYEALAQEIGEDAIRAPWDDYEREIAEEFLAHARAIR
                     QERRAAVAAGREPRIAEMLRHWGGVTIAYRRRLIDSPSYTLNHEEVEKALEEGIWFAE
                     GMTPVGVDVDHYGAARGIRLKNAGTGTEHWFPARTVFVAAGTQPNTVLAREHPEGFTL
                     DGRYFSAVDEHGNPVKPERGAAKPATVQVLLSREH"
BASE COUNT          424 a          921 c          938 g          385 t
ORIGIN      
        1 ccgcctggtc gcgctcggcc gccggatagg gattgttcca cgcgcccgtg cgcactccga
       61 tgaaggccag gccgagaacg gcaacaagcg cggcgccagc aaacgcccag cgcacggctg
      121 cggagcccgg cagtttcatg cggcccgact ccgggcttcg tccagtgcag tcaagcgtcg
      181 gttccgtggg cgggcgtgga tgttgcaatt gcgtgacgga atggtacttg cgcgtcccgg
      241 aatatgccac cgcggcgcgc ggtaaccttg cagcacggta aattgatcgc cgaaatgcac
      301 cgccttatag tggctcacct ccactgcggg actcccggat catgctcaag ctgtctttcg
      361 ggctcgattt tgcggacctc caccagcgcg agggcctggc acgcctcgac gcgcagtttc
      421 tccacttcat cgccacccct gaccccgcgc tgcgcgaggc gctgctcgcc gcgcgcgccg
      481 cgcccgaggc gctcgcgccg aaggccgaat cggaactgct gatcgcgctg gcgccgcagc
      541 tagaggactt cgtggcgcag ctgttcggca tcgaggccga ggcgcgcgcg ctggcgcaac
      601 agcaccacga gctcgcgccg gtattcagcg tcaagcggct gttcgtgcag cgccaggcac
      661 tggcgaagtt caagcccgag gacgttatcg atctcgacgc ggatgccacc gagcaggaac
      721 tgctgggcgg tccgttcagc gagctggcct tcgcgcgccg gatcacggcc tggcaggaag
      781 acgaggcggc caacgccggg cagctcgagc tggcggcgcg ctacgcggcg tgggcggccg
      841 gcaccgaggc cgggcgcgcg cgccatcgcg acggtgtgct gttcaaggcg cccgcgaagc
      901 tcgattacca gaacctcgtg ccgttgatca cgcgcgagac cctgggcttc ccgcgccaca
      961 ccatcgacca cctgcgccgg cgcgagggct tcgcgctgac cgaccacggc accgacctcg
     1021 cgggcgcgct cgacgaggcc aactactgca tctggtgcca caaccagggc aaggactcct
     1081 gctcgcgcgg cctgcgcgag aaggcgccca ggggcgccga tcccgccagc gcgccgtttc
     1141 gcaagtcgcc cttcggcgtc acgctggcgg gctgtccgct ggaggaaaag atctccgagt
     1201 tccacctggt caagacgcag ggccagccga tcgccgcgct ggcgatgatc gtggtcgaca
     1261 acccgatggc ggcagccacg gggcatcgca tctgcaacga ctgcatgaag tcctgcatct
     1321 accagaagca gcagccggtg gacattccgc aggcggaaac ccgcacgctg aaggacgtgc
     1381 tcgagctgcc ctggggcttc gagatctacg cgctgctgac gcgctggaac ccgctgaacc
     1441 tgcgccgccc gctgccgctc gcggataccg ggcgcaaggt gctggtggtc ggcctggggc
     1501 cggccggctt cacgctggcg catcacctga tgaacgacgg ccacacggtg gtcggcatcg
     1561 acgggctgaa gatcgagccg ctgcccgcgg aactctccgg cgtcgacctg cgcggcgccc
     1621 gcgtgcgctt cgaaccggtg cgcgacgtga agtcgctgat ggaaccgctg gatgcgcgcg
     1681 tgatggcggg cttcggcggc gtggccgaat acggcatcac ggtgcgctgg gacaagaact
     1741 tcctgaagct gatccgcctg ctggtggagc ggcgcagcgc cttcgcgatg ttcggcggcg
     1801 tgcgcttcgg cggcaccgtc accccggaag acgcgtggcg gatgggcttc gaccacatcg
     1861 cgctgtgcgt gggcgccggc ggcccgacgg tgctggatat cccgaacggg ctggcgcgcg
     1921 gcgtgcgcgc ggcatcggat ttcctgatgg cgctgcagct cacgggcgcg gccaagcccg
     1981 actccatcgc gaacatgcag ttgcgcctgc cggtggtggt gatcggcggc ggtctcaccg
     2041 cgatcgatac cgccaccgag tcgctggcct actacgtggt gcaggtcgag aagttcctgg
     2101 cgcgctacga ggcgctggcg caggagatcg gcgaggacgc gatccgcgcc ccgtgggacg
     2161 actacgagcg cgagatcgcc gaggagttcc tggcgcacgc gcgcgcgatc cgccaggaac
     2221 gcagggccgc ggtggcggcc gggcgcgaac cgcgcatcgc cgagatgctg cgtcactggg
     2281 gcggcgtcac catcgcctac cgccgccgcc tcatcgacag cccctcgtac acgctgaacc
     2341 acgaggaggt cgagaaggcg ctggaggaag gcatctggtt cgccgagggg atgacgccgg
     2401 tcggcgtcga cgtggaccac tacggtgcgg cgcgcggcat ccgcctgaag aacgccggca
     2461 ccggcaccga gcactggttc ccggcgcgca cggtgttcgt ggccgccggc acgcagccca
     2521 acaccgtgct ggcgcgcgaa cacccggaag gcttcacgct ggacggcagg tatttttcgg
     2581 ccgtcgatga gcacggcaac ccggtcaagc ccgaacgcgg cgccgccaag cccgccacgg
     2641 tgcaggtgct gctgtcacgc gaacacga
//
LOCUS       DAORUQ010000308         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_55990_length_2665_cov_5.914943, whole genome shotgun sequence.
ACCESSION   DAORUQ010000308 DAORUQ010000000
VERSION     DAORUQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55990_length_2665_cov_5.914943"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLW13_15870"
     CDS             complement(<1..715)
                     /locus_tag="PLW13_15870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY71907.1"
                     /translation="MSVAGHVASYYAASANAAPELAPLHGETRAEVCVIGGGYTGLST
                     ALHLAGRGSDVVLLEAERVGWGASGRNGGQASTGQRRPQRELEQRLGEGHARLLWELG
                     LEAVALVRELVERHRIACDLKPGILYAGWKQRDDDYLRDEATHLGERYGYTQCRHVPA
                     EEMRTLSGSTVFRGGLLDQGALHLHPLNYALGLAEAARRAGARMHEQSRVTGYDEGPR
                     IRVRTAQGSVLADHLVLACN"
     gene            919..2256
                     /locus_tag="PLW13_15875"
     CDS             919..2256
                     /locus_tag="PLW13_15875"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQY71908.1"
                     /translation="MDTIHKWLIDNRITEIEVLVPDMTGNARGKFVPADKFMKQESLR
                     LPEGILAQAVNGDYPDDYWELVDPRDRDMNLRADPGTMRVVPWANDATAQIIHDCYDM
                     EGNPHPLATRNVLRRVLALYEREGLKPVVAPEVEFYLVKKNADPDYELEPPLGRSGRP
                     ESARQSYSIDAVNEFDPIIEDMYDFCEAQKLDVDTLIHEAGAAQLEVNFLHGDALALA
                     DQVFTFKRTMRETALRHNVFATFMSRPMAKEPGSSMHIHQSILRLDDDTNIFSHADGS
                     DTEAFMHYIGGLQKYVPLLIALFAPNVNAYRRFTREISAPISLQWGYDNRTTGFRVPV
                     SDMRSRRVENRFAGADANPYLAIAASLVAGYLGMKQKLAPTAPCPGNAYEEEITVARS
                     LEEALRYIDEDDAVVDILGQQFIRAYRSVKLAEYDAFNRTISSWEREHLLLNV"
     gene            2386..>2665
                     /locus_tag="PLW13_15880"
     CDS             2386..>2665
                     /locus_tag="PLW13_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQY71909.1"
                     /translation="MTEIRPAATVILLRDAPDGLHTLLLRRNSALGFAGGNWVFPGGR
                     VDPEEIAAAADELEAARLAAVRETREEAELHIDIDSLRYYAHWTTPPIM"
BASE COUNT          460 a          925 c          851 g          429 t
ORIGIN      
        1 cgttgcaggc gagcaccaga tgatcggcga gcaccgagcc ctgcgcggtg cgcacgcgga
       61 tgcgtggtcc ttcgtcgtag ccggtcacgc gcgactgctc gtgcatgcgc gcgccggcgc
      121 gccgggcagc ctcggccagc cccagtgcat agttcagcgg gtgcaggtgc agcgcaccct
      181 gatcgagcag gccaccgcga aacaccgtgc tgcccgagag cgtgcgcatc tcctcggcgg
      241 gcacgtggcg gcactgcgta tagccgtagc gctcaccgag atgcgtcgct tcatcgcgca
      301 ggtagtcgtc gtcgcgctgc ttccagccgg catagaggat gccgggcttg aggtcgcagg
      361 cgatgcggtg gcgttcgacc agttcgcgca ccagcgcgac cgcctccagc ccgagttccc
      421 acagcaggcg cgcgtgcccc tcgccgagac gctgctcgag ctcgcgctgc gggcggcgct
      481 ggcccgtgct cgcctgtccg ccgttgcgcc cggaggcgcc ccaaccgacg cgctcggcct
      541 cgagcagcac gacatcgctg ccgcgcccgg cgaggtgcag cgcggtcgag agcccggtat
      601 agccgccgcc gatcacacag acctcggcgc gcgtttcacc gtgcagcggg gcaagttccg
      661 gcgccgcgtt ggcggaggcg gcgtaatacg aagccacgtg accggcgaca ctcatccgga
      721 ggttctcccg cgcggccggc ttgcttttgc ccggcgcccc tgcttaaact gaatcaatat
      781 tcgatttaag gcgaatgata attcggttac tgtgctgccg caaccccgtc gcgcgttgtc
      841 gtcgcggcca ctggcgcttg cggtctatca tggcaacgcc ggcaacgccc tgtttcatca
      901 cacgcgaaag tgaatcccat ggacaccatc cataaatggc tgatagacaa ccgcatcacc
      961 gagatcgagg tgctggtgcc ggacatgacc ggcaatgcgc gaggcaagtt cgtgcccgcg
     1021 gacaagttca tgaagcagga aagcctgcgc ctgcccgagg gcatcctcgc ccaggcggtc
     1081 aacggggatt accccgacga ctactgggag ctggtcgatc cgcgcgaccg cgacatgaac
     1141 ctgcgtgccg accccggtac catgcgggtt gtgccgtggg ccaatgacgc gaccgcgcag
     1201 atcatccacg attgctatga catggaaggc aatccccatc cgctggcgac gcgcaacgtg
     1261 ctgcggcgcg tgctcgcgct ctacgagcgc gaaggcctga agcccgtggt ggcgcccgag
     1321 gtcgagttct acctggtcaa gaagaacgcc gatcccgact acgaactgga gccgccgctg
     1381 gggcgctcgg gccggccgga atccgcgcgc cagtcctact ccatagacgc cgtcaacgag
     1441 ttcgatccga tcatcgagga catgtacgat ttctgcgagg cgcagaaact ggacgtggac
     1501 acgctgatcc acgaggccgg cgcggcgcag ctcgaggtca acttcctgca cggcgatgcg
     1561 ctggcgctcg ccgaccaggt gttcaccttc aagcgcacca tgcgcgagac cgcgctgcgc
     1621 cacaatgtct tcgcgacctt catgtcgcgc ccgatggcca aggagccggg cagttcgatg
     1681 cacatccacc agagcatcct gcgcctcgat gacgacacga acatcttcag ccatgccgat
     1741 ggcagcgaca ccgaagcgtt catgcactat atcggcgggc tgcagaagta tgtgcccctg
     1801 ctcattgcgc tgttcgcgcc gaacgtcaat gcctaccggc ggttcacgcg cgagatatcc
     1861 gcgccgatca gcctgcaatg gggttacgac aaccgcacca ccggatttcg cgtgccggtg
     1921 agcgacatgc gctcgcgccg cgtggagaac cgctttgccg gtgccgatgc caacccctac
     1981 ctcgcgatcg ccgcgagcct ggtggccggt tacctcggca tgaagcagaa gctcgcgccc
     2041 acggcgcctt gccccggcaa cgcctatgaa gaggagatca ccgtggcgcg cagcctcgag
     2101 gaagcgctgc gctacatcga cgaggacgac gcggtggtcg acatcctcgg tcagcagttc
     2161 atccgcgcct accgctcggt gaagctggcc gagtacgacg ccttcaaccg caccatttcg
     2221 tcctgggagc gcgagcacct gctgctgaac gtctgagtgc tgcgccaccc gccgctgccg
     2281 ccccgccgca cccgggggac agtgggagcg cgcgcttaac cagagttaat gtcgcaacgc
     2341 ccgtcgcatg tcttatagtc gcaggcctcc aacgcctggt gcctcatgac agaaatccgc
     2401 cctgccgcca ccgtcattct cctgcgcgat gcacccgacg ggctgcatac gctgctgttg
     2461 cgccgtaatt ccgcgctcgg cttcgccggc ggcaactggg tgtttcccgg cggccgcgtg
     2521 gatcccgagg agatcgccgc cgcggcggac gagctcgagg ccgcgcgcct ggcggcggtg
     2581 cgcgagacgc gcgaggaggc ggaactgcac atcgacatcg attcgctgcg ctactacgcg
     2641 cactggacca cgccgccgat catgc
//
LOCUS       DAORUQ010000309         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_55992_length_2665_cov_5.077778, whole genome shotgun sequence.
ACCESSION   DAORUQ010000309 DAORUQ010000000
VERSION     DAORUQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55992_length_2665_cov_5.077778"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLW13_15885"
     CDS             <1..1318
                     /locus_tag="PLW13_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY71910.1"
                     /translation="ADAINDSKGAALAVDGALLPRLQQVRDRVAFGHLLLHRSMPEQV
                     REAGAAATLAELATHPDVEPDMSYQHYGDTAAVLWTSGTTGKSKGVMQSHNVWIRAAE
                     NANRHFGTTDGDIAYNCLPLYNSAAWVANIYRALVAGIPCAIDDHFSVHAFWDRIRFY
                     RATQTMTLGAMHMFLWNEPPRADDADNPLRMANMVPMPERLLHPFCERFGIEGITQGF
                     GQSEIMLMLSRKDTPQKQWTPNALGELSQGVELRMLKDDGSEAAPGEVGEFCVKETSP
                     HTIFNGYFDNPQANEAAFAGGWYHTGDLGIRDADGDYFFVDRKKDVIRYKGRNVSSLQ
                     VESVAMQHPAVQAAAVYGVASQELDSEHEIKLDAVPRPGAQVSAEELARFINANAPYF
                     CVPRYIEFVGELPYTPTNKVQKYKLRERGVTPQSWDRVAAGFKIER"
     gene            1360..1653
                     /locus_tag="PLW13_15890"
     CDS             1360..1653
                     /locus_tag="PLW13_15890"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HQY71911.1"
                     /translation="MLKSVSLISFKEGTDQERREAVLAAYRKLPSLIPGIVSFEVGLD
                     QGLLEGNAGIAVIVGFAAREDFLAYSTHQAHTDVIFPVCGPVMAGWSSVQFDA"
     gene            complement(1727..>2665)
                     /locus_tag="PLW13_15895"
     CDS             complement(1727..>2665)
                     /locus_tag="PLW13_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY71912.1"
                     /translation="INGIDMHYVEQGQGPLVVLCHGFPHLWFSWRHQIPVLAAAGWRV
                     VAPDMRGMGQTEAPAAIEAYGVDRICDDLTGLLDHLGEASAIFAGLDFGAFAIYDLAL
                     RDPGRVRAVIGLENPAAPHNPDVPPLSEYAAMARQHFVHIEYFREPGPADAALDAAPR
                     EFLRKVFHALSGAYHYLDVWKHPPGTAYLDALPETPPLPWPWLSDLELEFFVSEYARS
                     GFTGGLNYYRSMDLKWRQRKPYEGVQSPVPAFFIGSELDCDLEGFHGPDPIALMRSQF
                     PDLRAVEMIPGAGHLVQMEKPDEVNRCLLRFLETLR"
BASE COUNT          452 a          888 c          911 g          414 t
ORIGIN      
        1 ggcggatgcg atcaacgaca gcaagggagc ggcgctggcg gtggatggcg cgctgctgcc
       61 gcgcctgcag caggtgcgcg atcgcgttgc cttcggccac ctgctgctgc atcgcagcat
      121 gccggagcag gtgcgcgagg ccggcgccgc ggccacgctg gccgagctcg cgacgcaccc
      181 cgacgtcgag cccgatatga gctaccagca ctacggcgac accgccgcgg tgctgtggac
      241 ctcgggcacc accggcaagt ccaagggcgt gatgcagagc cacaacgtgt ggatccgtgc
      301 cgccgagaac gccaaccgcc atttcggcac caccgatggc gacatcgcct acaactgcct
      361 gccgctgtac aactcggccg cgtgggtcgc caatatctac cgcgcgctgg tggcgggcat
      421 cccgtgcgcg atcgatgacc atttctcggt gcacgcgttc tgggaccgta tccgtttcta
      481 ccgcgccacc cagaccatga ccctcggtgc gatgcacatg ttcctgtgga acgaaccgcc
      541 gcgcgccgac gacgccgaca acccgctgcg catggcgaac atggtgccca tgcccgagcg
      601 tctcctgcac ccgttctgcg agcgcttcgg catcgagggc atcacgcagg gcttcggcca
      661 gagcgagatc atgctgatgc tctcgcgcaa ggacacgcca cagaagcagt ggacgcccaa
      721 tgcgctgggc gagctctcgc agggcgtgga gctgcgcatg ctcaaggacg acggcagcga
      781 ggccgcgccc ggcgaagtcg gggagttctg tgtgaaggag acttcaccgc acacgatttt
      841 caacggctac ttcgacaacc cgcaagcgaa tgaagcggct tttgccggcg gctggtatca
      901 caccggggac ctcggcatcc gcgatgccga cggcgactat ttcttcgtcg accgcaagaa
      961 ggacgtgatc cgctacaagg ggcgcaacgt gtcctcgctg caggtcgaga gtgtcgcgat
     1021 gcagcacccg gcggtgcagg cagcggcagt ctacggcgtg gcatcgcagg aactggactc
     1081 cgagcacgag atcaagctcg acgcggtgcc caggccgggc gcgcaggtca gcgcggagga
     1141 gcttgcgcgc ttcatcaacg cgaacgcgcc gtacttctgc gtgccgcgct acatcgagtt
     1201 cgtgggcgaa ctgccctaca cgccgaccaa caaggtgcag aagtacaagc tgcgcgagcg
     1261 cggcgtgacg ccgcagagct gggaccgcgt ggcggccggc ttcaagatcg agcgctgagg
     1321 cgcggcgcca ggatgacacc aaccgcagga gagaagatca tgctcaagag cgtttcgctc
     1381 atcagcttca aggaaggcac ggaccaggaa cggcgtgaag cggtgctcgc ggcctaccgc
     1441 aagctgccct ctctcattcc cggcatcgtg tccttcgagg tcgggctgga ccagggcctg
     1501 ctggaaggca atgccggcat cgccgtgatc gtcggctttg ccgcgcgcga ggatttcctt
     1561 gcctattcga cgcaccaggc ccacaccgat gtcatctttc cggtctgcgg cccggtgatg
     1621 gcgggttggt cctccgtgca gttcgatgcc tgaaccgtgc tgcgacgcca gccccgtggc
     1681 ttgtgctccg gcatgagcgc ggtcggcgtg gcggtgtgcg ccgccgctag cgcagcgtct
     1741 ccaggaagcg cagcaggcag cggttgacct cgtcgggctt ctccatctgc accaggtggc
     1801 cggcgccggg gatcatctcc acggccctca ggtcggggaa ctgcgagcgc atcagcgcga
     1861 tggggtccgg gccgtggaat ccctccaggt cgcagtcgag ctcgctgccg atgaagaagg
     1921 ccggcaccgg gctctgcaca ccctcgtacg gcttgcgctg gcgccacttc aggtccatcg
     1981 agcggtagta gttgagtccg ccggtgaaac cgctgcgcgc atattccgag acgaagaact
     2041 ccagttccag gtcgctcagc cacggccagg gcaggggcgg cgtctcgggc agcgcgtcga
     2101 ggtacgcggt gcccggcggg tgcttccata cgtcgaggta gtggtaggcg cccgaaagcg
     2161 cgtggaacac cttgcgcagg aactcgcgcg gtgcggcgtc cagcgccgca tcggccgggc
     2221 cgggctcgcg gaagtactcg atgtgcacga agtgctgcct ggccatcgcc gcgtattcgc
     2281 tcaggggcgg cacatcggga ttgtgcggcg cggccgggtt ctccagcccg atcaccgcgc
     2341 gcacgcggcc cgggtcgcgc agcgccaggt cgtagatcgc gaaggcgccg aaatccagtc
     2401 ccgcgaagat cgcggaagcc tcgccgaggt gatcgagcag ccccgtgagg tcgtcgcaga
     2461 tgcggtcgac gccgtaggcc tcgatcgccg ccggcgcctc ggtctggccc atgccgcgca
     2521 tgtcgggggc gaccacgcgc cagcctgccg ccgcgagcac cgggatctgg tggcgccagc
     2581 tgaaccacag gtgcgggaaa ccgtggcaga gcaccaccag cgggccctgt ccctgctcca
     2641 cgtaatgcat gtcgatgccg ttgat
//
LOCUS       DAORUQ010000310         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_56100_length_2662_cov_6.451477, whole genome shotgun sequence.
ACCESSION   DAORUQ010000310 DAORUQ010000000
VERSION     DAORUQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56100_length_2662_cov_6.451477"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1843)
                     /locus_tag="PLW13_15900"
     CDS             complement(59..1843)
                     /locus_tag="PLW13_15900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQY71913.1"
                     /translation="MNSIAERLGAVREQMRQRGLDALIVPRADEYLGEYIPARNERLR
                     WISGFTGSAGVVVVLADAAAIFVDGRYTVQVRQQVSEQLFEVLHLVDEPHARWLGKRL
                     GEGRRVGYDPRMHTLAWQKKTADTLARSGVELLALEENLIDLCWRDRPQPERHPAILL
                     EMRYTGKSSADKRREIGAAIAGQGADAALVFAADSVAWLLNIRGSDVPHLPLVLGHAL
                     LHADGRLAFFTDPLKIPAGFEAHTGAGVEVLAESEADASFRALAGRRVLADPDTANAW
                     CQLRLAAHGAELVAGADPVLLPKACKNAAEIAGMRAAHIRDAVAEIRFLAWLDAEVAA
                     QRLHDEATLCERLHAFRAADPLLKDLSFDTISAAGGNAAMCHYRHSDTDPGRLEMNSV
                     YLVDSGAQYLDGTTDITRTVAIGDAGAEIRRMATLVLKGHIALDQARFPKGTTGTQLD
                     VLARQFLWREGFDYDHGTGHGVGSFLSVHEGPQRIGKACSAAELSPGMVVSNEPGYYK
                     ADCYGIRHENLLLVKELGQQPGADRAMYGFEALTLVPFDRALLDPALMTGEEIAWLNA
                     YHRRVRETIGPLLDGSDRAWLENATREL"
     gene            complement(2066..>2662)
                     /locus_tag="PLW13_15905"
     CDS             complement(2066..>2662)
                     /locus_tag="PLW13_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY71914.1"
                     /translation="PKLTIEADANHDGVYEAFTAKLATAVAQFRLGDGAQDGINMGPL
                     IDEGAANEVMSFVEDAVAKGATVVTGGTRGSLGGSFVEPTILTNVSTDMRVFNEEIFG
                     PVAPVFRFSTEAEAIALANDTPFGLASYFYARDIGRIWCVAEGLEYGIVGINEGIIST
                     EVAPFGGVKESGSGREGSKYGMDDYLEIKYLCMGGIDR"
BASE COUNT          386 a          900 c          926 g          450 t
ORIGIN      
        1 tgctcgggca tagtgtgatt cacgcggcgg agtttttaca caggctcttc atgccgacct
       61 acagttcgcg cgtcgcgttc tccagccacg cgcggtcgct gccgtccagc aacggcccga
      121 tggtctcgcg cacgcgccgg tggtaggcat tcagccacgc gatctcctcg cctgtcatca
      181 gcgcggggtc cagcagcgcg cggtcgaagg gcaccagcgt cagcgcctcg aaaccgtaca
      241 tcgcgcggtc ggcgccgggc tgctgcccga gctccttcac cagcaggagg ttctcgtggc
      301 ggatgccgta gcagtcggcc ttgtagtagc ccggttcgtt gctcaccacc atgccgggac
      361 tgagctcggc ggcgctgcag gccttgccga tgcgttgtgg cccctcgtgc acgctgagga
      421 agctgccgac gccgtggccg gtaccgtggt cgtagtcgaa gccctcgcgc cacaggaact
      481 ggcgcgccag cacatcgagc tgcgtgccgg tggtgccctt ggggaagcgc gcctggtcga
      541 gcgcgatgtg gcccttcagc accagcgttg ccatgcgccg gatctccgcg cccgcatccc
      601 cgatcgcgac ggtacgcgtg atatcggtgg tgccgtcgag gtactgcgcg ccgctgtcca
      661 cgaggtagac gctgttcatc tccaggcgtc cgggatcggt gtcggagtgt cggtagtggc
      721 acatcgcggc gttgccgccg gccgcggaaa tggtgtcgaa gctcaggtcc ttcagcagcg
      781 ggtcggcggc acggaaagcg tggagccgtt cgcacagcgt cgcctcgtcg tgcaggcgtt
      841 gcgccgcgac ctcggcgtcg agccaggcga ggaagcggat ctcggcaacc gcatcgcgga
      901 tatgcgcggc gcgcatgccg gcaatctcgg cggcgttctt gcacgccttg ggcagcagca
      961 ccggatcggc gccggcgacc agttcggcgc cgtgcgccgc gaggcgcagc tggcaccagg
     1021 cgttggccgt gtccggatcc gccagcacgc gcctgccggc gagggcgcgg aacgaggcat
     1081 ccgcctcgct ctcggcgagc acctcgacgc ccgcgccggt gtgcgcctcg aagcccgcgg
     1141 gaattttcag cgggtcggtg aagaacgcga gcctgccgtc ggcgtggagc agggcatggc
     1201 ccagcaccag cggcaggtgc ggcacgtcgc tgccgcggat gttcagcagc caggccaccg
     1261 agtcggccgc gaacaccagc gcggcgtcgg cgccctgccc ggcgatggcc gcgccgatct
     1321 cgcgccgctt gtccgcgctc gacttgccgg tgtaacgcat ctccagcagg atcgcgggat
     1381 ggcgctcggg ctgcgggcga tcacgccagc acaggtcgat gagattctcc tccagcgcca
     1441 gaagttcgac gccgctgcgc gccagcgtgt cggcggtctt cttctgccac gccagcgtgt
     1501 gcatgcgcgg gtcgtagccg acgcgcctgc cttcgccgag ccgcttgccc agccagcgcg
     1561 cgtgcggctc gtcgaccaga tggaggactt cgaacagttg ctccgacacc tgctggcgaa
     1621 cctgcacggt gtagcggcca tcgacgaaga tcgccgccgc gtcggcgagc acgaccacca
     1681 cgcccgccga gccggtgaag ccgctgatcc agcgcagccg ctcgttgcgc gccgggatgt
     1741 attcgccgag gtattcgtcg gcgcgcggca cgatcagcgc gtcgagaccg cgctggcgca
     1801 tctgctcgcg caccgcgccg agtcgttctg caatcgagtt cattgacgtg gatttcctgg
     1861 ggtctcttgc tttcgttccg atggggcgcg ccgaaaatac taacccatcg cggcgggtgg
     1921 caggtctatc gtcctgcccg gttcagcagc tcttcgtgag ctgcctgcgg acgtgtcggc
     1981 tgcggtgccg ggcgcgatcg caccagcgcg cgtccatcca gcggcggatg gatttgcaag
     2041 gcgtgcgccc caatgttcag aggaatcagc ggtcgatccc gcccatgcac aggtacttga
     2101 tctcgaggta gtcgtccatg ccgtacttgg agccctcgcg gccggaaccg gactccttca
     2161 cgccgccgaa gggggcgacc tccgtggaga tgatgccctc gttgatgccg acgatgccgt
     2221 attccaggcc ttcggccacg caccagatgc ggccgatgtc gcgcgcgtag aaatacgagg
     2281 cgagaccgaa aggcgtgtcg ttggcaagcg cgatggcctc ggcctcggtg gagaagcgga
     2341 acaccggtgc gaccgggccg aagatctcct cgttgaagac gcgcatgtcg gtcgagacat
     2401 tggtgaggat ggtcggctcc acgaaactgc cgccgaggct gccgcgcgtg ccgccggtga
     2461 ccacggtcgc gcccttggcg acggcgtcct cgacgaagct catcacctcg ttggcggcgc
     2521 cctcgtcgat cagcggtccc atgttgatgc cgtcctgcgc accgtcgccg aggcgaaact
     2581 gcgcgacggc ggtggcgagc ttcgcggtga aggcctcgta gacgccgtcg tggttggcgt
     2641 ccgcctcgat cgtgagcttc gg
//
LOCUS       DAORUQ010000311         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_56173_length_2660_cov_6.573512, whole genome shotgun sequence.
ACCESSION   DAORUQ010000311 DAORUQ010000000
VERSION     DAORUQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56173_length_2660_cov_6.573512"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /gene="mutL"
                     /locus_tag="PLW13_15910"
     CDS             complement(<1..1652)
                     /gene="mutL"
                     /locus_tag="PLW13_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQY71915.1"
                     /translation="MGRIRQLSDTLANQIAAGEVVERPASVVKELVENSLDAGASRVE
                     IDLEDGGMRLIRIRDDGEGIAADDLPLTVSRHATSKIRTLDDLEKVLSLGFRGEALAS
                     IASVARFGISTATADAPHGSRLEFAASGEPRISPTAHPRGTTVEVHDLFFNTPARRKF
                     LRAERTEYGHCEEVVRRLALGRFDAGFTLRHNQKATFALRACGTPAEQEQRVATLCGA
                     PFLQNALALEVEASGLLLRGWVAQPAFSRSQADLQYFFVNGRVVRDRLVSHAVRQAYR
                     DVMYHDRHPAFVLYLEIDPALVDVNVHPTKSEVRFRESRMVHDFLFHTLHRALAQARP
                     GAAEAPARTAAIEAAAAAAPVAMPAAQHALWQRPDAAALAETVAAYRALGATTGAAAL
                     PSAPARGPGESDGDVPPLGYAIAQLKGIYILAENRDGLVVVDMHAAHERITYERMKAA
                     RDAGGVRAQPLLVPQVVAVSRREADCVEQHAPALAELGLELARAGEEAVMVRQIPVLL
                     AGADIAALVRDVIADLLEYGSSARIEAHADEILAGMACHCSVR"
     gene            complement(1696..>2660)
                     /locus_tag="PLW13_15915"
     CDS             complement(1696..>2660)
                     /locus_tag="PLW13_15915"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQY71916.1"
                     /translation="AQPEPQPVARSEEPAPVVRAPLPKQPLRGRSILVAIDAGHGGED
                     PGAIGPGRIREKDVVLAIARELEKKVNATPGYRAMLVRKGDYFIPLFKRRDIARQSGA
                     DLFVSIHADAFTHSRARGSSVYALSPRGATSVSAAFLAQRENGADLIGGVDLGAKDKV
                     LASVLTDLSMTATMDASLGAGGRVLHSMGRISHLHKRRVEQAGFAVLKSPDMPSILVE
                     TGFISNPLEAERLGNAGYQRNMAAAIFAGVSDYFMSSPPPETMVAMSVRAGARPSEYI
                     ISRGDTLAGIAERYRVSVADIVRTNGLGGGNDIRAGQTIVIPQT"
BASE COUNT          382 a          943 c          950 g          385 t
ORIGIN      
        1 cgcaccgagc aatggcaggc catgccggcg aggatctcgt cggcatgcgc ctcgatgcgc
       61 gcgctgctgc cgtactccag caggtccgcg atcacgtcgc gcaccagcgc ggcgatatcg
      121 gcgcccgcga gcagcacagg tatctggcgc accatcaccg cctcctcgcc ggcccgcgcg
      181 agctcgagcc cgagctcggc cagcgccggc gcgtgctgct ccacgcaatc ggcctcgcgc
      241 cggctgaccg cgaccacctg cggcaccagc agcggctgcg cgcgcacgcc gccggcatcg
      301 cgcgcggcct tcatgcgctc gtaggtgatg cgctcgtgcg ccgcgtgcat atcgacgacc
      361 accagcccgt cgcggttctc ggccaggatg tagatgcctt tcagctgcgc gatcgcgtag
      421 ccgagcggcg gcacgtcgcc gtcactctcc ccggggcccc gggcgggcgc cgacggtagc
      481 gccgcggcac cggtcgtggc gccgagcgcg cggtaggcgg ccacggtctc cgcgagcgcc
      541 gccgcatcgg ggcgctgcca cagcgcgtgc tgggcggccg gcatcgcgac cggcgccgcg
      601 gccgcggcgg cctcgatcgc cgccgttcgc gccggcgcct cggcagcgcc cgggcgcgcc
      661 tgcgccagcg cgcggtgcag ggtgtggaac aggaagtcgt gcaccatgcg cgactcgcga
      721 aagcgcacct cgctcttggt cggatgcacg ttcacgtcga ccagcgccgg gtcgatctcg
      781 aggtagagca cgaaggccgg gtggcggtcg tggtacatca cgtcacggta ggcctggcgc
      841 acggcgtgcg agaccaggcg atcacgcacc acgcgcccgt tcacgaagaa gtactgcagg
      901 tcggcctggc tgcgcgagaa cgccggctgc gcgacccagc cgcgcagcag cagcccggag
      961 gcctcgacct cgagcgcgag cgcgttctgc aggaagggtg ccccgcacag cgtggcgacg
     1021 cgctgttcct gctcggccgg cgtgccgcag gcacgcagcg cgaaggtggc tttctggttg
     1081 tggcgcagcg tgaagccggc gtcgaagcgc cccagcgcca gccggcgcac gacttcctcg
     1141 cagtggccgt actcggtgcg ctcggcgcgc aggaacttgc gccgggcggg cgtattgaag
     1201 aacaggtcgt gcacctcgac cgtggtgccg cgcgggtgcg ccgtgggcga gatgcgcggc
     1261 tcgccgctgg cggcgaactc cagccggctg ccgtggggtg cgtccgcggt ggcggtgctg
     1321 ataccaaagc gcgcgaccga ggcgatgctc gccagcgcct cgccgcgaaa accgaggctg
     1381 agcaccttct cgagatcgtc gagcgtgcgg atcttgctgg tggcgtgacg cgagacggtc
     1441 agcggcaggt cgtcggccgc gataccctcg ccatcgtcgc ggatgcggat gagccgcatg
     1501 ccgccgtcct cgagatcgat ctcgacgcgg ctggcaccgg catcgaggct gttctcgacc
     1561 agttccttga ccacggacgc aggccgttcc acgacctctc cggccgcgat ctggttggca
     1621 agcgtgtcac tgagctgccg gatgcgcccc atgctggaat caccgcgctg agcggaaggg
     1681 cgcgaaagcg gatgatcagg tctgcgggat cacgatggtc tgtcccgccc ggatgtcgtt
     1741 tccaccgccc aacccgttgg tcctgacgat gtcggcgaca ctaacacgat agcgttcggc
     1801 gattccagcc agggtgtcgc cacgcgagat gatgtactcc gaggggcgtg cgccggcacg
     1861 cacgctcatc gcgaccatcg tctcgggggg cgggctgctc atgaagtagt cgctcactcc
     1921 ggcaaagatc gccgcggcca tgttgcgctg gtagccggca ttgcccaggc gctcggcctc
     1981 gagcgggttc gagatgaatc cggtctcgac caggatcgag ggcatgtccg gggacttcag
     2041 caccgcgaag ccggcctgct cgacgcgcct cttgtgcagg tgcgagatgc gtcccatcga
     2101 gtgcagcacg cgcccgcccg cgccgaggct ggcatccatc gtggccgtca tcgacaggtc
     2161 ggtcagcacg ctggcgagca ccttgtcctt ggcgccgaga tccacgccgc ctatcaggtc
     2221 cgcaccgttc tcgcgctggg ccaggaaggc ggccgaaacg ctggtcgcgc cacgcggcga
     2281 caacgcgtag accgaactgc cgcgggcgcg cgagtgcgtg aaggcgtcgg cgtggatcga
     2341 gacgaacagg tcggcgccgg actggcgcgc gatgtcgcgc cgcttgaaca gcggaatgaa
     2401 gtaatcgccc ttgcgaacca gcatggcgcg gtagccgggc gtcgcgttga ccttcttctc
     2461 gagctcccgg gcgatcgcca gcaccacgtc cttctcgcgg atgcggcccg gcccgatggc
     2521 acccggatcc tcaccgccgt gcccggcgtc gatcgcgacc aggatactgc gaccgcgcag
     2581 cggctgtttc ggcagcggcg cgcgcacgac cggggcgggc tcctcgctac gcgccaccgg
     2641 ctgcggctcg ggctgagccg
//
LOCUS       DAORUQ010000312         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_56515_length_2650_cov_7.090173, whole genome shotgun sequence.
ACCESSION   DAORUQ010000312 DAORUQ010000000
VERSION     DAORUQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56515_length_2650_cov_7.090173"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..645
                     /locus_tag="PLW13_15920"
     CDS             40..645
                     /locus_tag="PLW13_15920"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQY71917.1"
                     /translation="MILMIDNYDSFTWNVVQYLGELGAEVKVVRNDEIAADAIEALAP
                     AKIVISPGPCTPNEAGISLEVIRRFAGRIPILGVCLGHQCIGQAFGGRIVRARQVMHG
                     KTSLIHHAGTGVFRGLANPFQATRYHSLVIEKESCPERLEVTAWTVDEHGRLDEIMGV
                     RHRDLAIEGVQFHPESILTEHGHALLANFLGVAWPTLARAS"
     gene            649..1686
                     /gene="trpD"
                     /locus_tag="PLW13_15925"
     CDS             649..1686
                     /gene="trpD"
                     /locus_tag="PLW13_15925"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQY71918.1"
                     /translation="MDIKQALDSVVNHIDLDRDAMREVMRAVMTGGCTDAQIGALLTG
                     LRMKGESIDEITAAAEVMRELATPVDVDVEPLVDIVGTGGDGASLFNISSASAFVVAA
                     AGGHVAKHGNRGVSSASGSADALEHLGIKLGMPAAMVARGIRELRVGFMFAPAHHGAM
                     RHAIGPRRELGMRTLFNILGPLTNPAGVRRLLVGTFSKALCRPMAEVLAQLGARHVMV
                     VHAEDGLDEFSLATRTHAVELREGAITERSFSPEELGVQSQSLIGLEVGSGAESAALI
                     RDALGKRRGPHAAKAADIIALNAGAAIYVSGITDSVRRGVELAHDMIHCGLALERIEA
                     LASFCACLETE"
     gene            1683..2486
                     /gene="trpC"
                     /locus_tag="PLW13_15930"
     CDS             1683..2486
                     /gene="trpC"
                     /locus_tag="PLW13_15930"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQY71919.1"
                     /translation="MSKATILLRILQRKREEVAERGRVRSLAELRALAREAAPVRGFA
                     DALATRVAAGRNAVIAEIKKASPSKGLIRADFDPPALARSYEQGGAACLSVLTDVDFF
                     QGADAYLQAARAAVALPVLRKDFTVDAYQVVEARALAADCILLIAAAFTDAPSHMAEL
                     AALATELGLDVLVEVHNAAELEMALAVPGRLIGINNRDLHSFEVSLDTTYALLDRIPA
                     DRIVVTESGIGTAEDVAAMRAHGVHAFLVGESLMRAPDPGERLRELFGT"
BASE COUNT          416 a          890 c          934 g          410 t
ORIGIN      
        1 gaggtatcta ttcaatgaaa tcaattcgat aggtcgaata tgattctgat gatcgacaac
       61 tacgactcct tcacctggaa cgtggtgcag tacctcgggg agctgggcgc cgaggtgaag
      121 gtcgtgcgca acgacgaaat cgcggctgac gccatcgagg cgctcgcgcc agcgaagatc
      181 gtgatctcgc ccgggccctg cacgcccaac gaggcaggca tctcgctcga ggtgatccgg
      241 cgcttcgccg gacgcatccc catcctcggt gtctgcctcg ggcaccagtg catcgggcag
      301 gccttcggcg ggcgcatcgt gcgcgcgcgc caggtcatgc acggcaagac ctcgctgatc
      361 caccacgccg gcacgggcgt gttccgcggg ctggcgaacc cgttccaggc cacgcgctac
      421 cactcgctgg tgatcgagaa ggagtcctgc cccgagcgcc tcgaagtcac ggcctggacc
      481 gtggacgagc acgggcgcct ggacgagatc atgggcgtgc ggcaccgcga tctcgcgatc
      541 gagggcgtgc agttccaccc cgagtccatc ctcacggagc acgggcacgc gctgctggcg
      601 aattttctcg gcgtggcctg gcccaccctg gcgcgggcga gctgagccat ggatatcaag
      661 caggcactgg atagcgtcgt caatcacatc gacctcgatc gcgacgcgat gcgcgaggtc
      721 atgcgcgcgg tcatgacggg cggctgcacc gacgcacaga taggcgcgtt gctgacgggc
      781 ctgcgcatga agggcgagtc gatcgacgag atcaccgccg cggccgaggt catgcgcgag
      841 ctcgccacgc cggtcgacgt cgatgtcgag ccgctggtcg acatcgtggg gaccggcggc
      901 gacggcgcca gcctgttcaa catctccagc gcctcggcct tcgtggtcgc cgcggccggc
      961 ggccatgtcg cgaagcacgg caatcgcggc gtctccagtg cgagcggcag cgccgacgcg
     1021 ctggagcacc tgggtatcaa gctcgggatg ccggcggcga tggtcgcgcg cggcatccgc
     1081 gaactgcgcg tgggcttcat gttcgcgccg gcgcaccacg gcgcgatgcg ccacgcgatc
     1141 gggccgcgac gcgagctcgg catgcgcacg ctgttcaaca ttctcgggcc gctgaccaac
     1201 ccggccggcg tgcgccgcct gctggtcggc accttcagca aggcgttgtg ccggccgatg
     1261 gccgaggtgc tggcgcagct gggcgcgcgc cacgtgatgg tggtacatgc cgaggatggc
     1321 ctggacgagt tcagcctggc aacgcgcacg cacgccgtcg agttgcgcga gggcgccatc
     1381 accgagcgca gcttttcgcc cgaggagctc ggtgtgcaga gccagagcct gatcgggctc
     1441 gaggtcggct cgggtgccga gtcggcggcc ttgatccgcg atgcgctcgg caagcgccgc
     1501 gggccgcatg cggccaaggc ggccgacatc atcgcgctga acgccggtgc cgcgatctac
     1561 gtgagcggca tcaccgattc cgtgcgccgc ggcgtcgagc tcgcgcacga catgatccat
     1621 tgcggccttg cgctcgaacg tatcgaggcg ctggcgagct tctgtgcatg cctggagact
     1681 gaatgagcaa ggcgaccatc ctgctgcgga tcctgcagcg caaacgcgag gaagtcgccg
     1741 agcgtggccg cgtgcgcagc ctcgccgagc tgcgtgccct ggcgcgtgag gccgcgccgg
     1801 tgcgcggctt tgccgatgcg ctggcaacgc gcgttgctgc cgggcgcaac gccgtgatcg
     1861 ccgagatcaa gaaggcctcg cccagcaagg gcctgatccg cgcggatttc gatccgcccg
     1921 cgctggcgcg ctcctacgag cagggcggcg cggcgtgcct gtcggtgctg accgacgtcg
     1981 atttcttcca gggagcggat gcctacctgc aggcggcgcg cgcggcggtc gcgttgccgg
     2041 tattgcgcaa ggacttcacc gtcgatgcct accaggtcgt cgaggcgcgc gcactcgcgg
     2101 ccgactgcat cctgctgatc gccgcggcct tcaccgatgc gccgtcacac atggcggagc
     2161 tggcggcgct ggcgaccgag ctcgggctgg acgtgctggt cgaggtgcac aacgccgccg
     2221 agctcgagat ggcgctcgcc gtgcccgggc ggctgatcgg catcaacaac cgcgacctgc
     2281 acagcttcga ggtctcgctg gacaccacct acgcgctgct ggatcgcatc ccggccgacc
     2341 gtatcgtggt gaccgagagc ggcatcggca cggccgagga tgtggcggcg atgcgcgcac
     2401 acggcgtgca cgccttcctc gtcggcgaga gcctgatgcg cgcgccggac ccgggcgagc
     2461 ggctgcggga actgttcggc acttagttcg ggcccgcagg tcgggtttca acccggcaag
     2521 gattcggcag cacccgtggg tcgccatgaa tggcgacagc agaatccggg caggcacccg
     2581 tcggcatgaa tgccgaccca cttcagcggg tgccgaacac caccatggtc ttgcccttga
     2641 cgtgaacgag
//
LOCUS       DAORUQ010000313         2594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_58598_length_2594_cov_5.570697, whole genome shotgun sequence.
ACCESSION   DAORUQ010000313 DAORUQ010000000
VERSION     DAORUQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58598_length_2594_cov_5.570697"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLW13_15935"
     CDS             <1..1910
                     /locus_tag="PLW13_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY71920.1"
                     /translation="FQRLRIPTSLGYLLVGLILGPHTAGPALQTAPIQALAEFGIVFL
                     LFTVGLNYSLPQIHALRNTVLTLGTAQVGLTTLVVGLLAYFVLGLPAATAFVIGAVFA
                     QSSTTVISNQLIAQGEDNSRHGRLGVAMSVFQDVTAVPLLILIPVFGAAAGFTTVLGD
                     IALALAKAVLAFVVVLYGGRWLLRPLFHYVAGQRSPELFTLTVLLVSLAAGWATGKLG
                     LSMAFGAFLAGMMLGETEFRHQVEATIRPFRDVLLGLFFVGVGMLIDPMVLPGIWHWV
                     LIGTAVLLLSKILLVAQIVRWSGVDPLTAWSTALLLAVGGEFGFAVLSISLGDGAIDS
                     HTGQIVLYSVLLSIIVAPFLIRYNHAIATWLSPAVTAAVKPGVPGASAEQAGKLDEHV
                     VICGYGRMGQSVGNFLEEEGIPYVALDLDSDRVREAHLAGEPVFYGDASDGSVLDALG
                     IARARLLVISHNDIAAARRILHHVRRQRPELPVMVRTIDETHVEELRAAGATEVIPET
                     LEASLMVASAALLLLDVPLVRVVQKMREQRNSHYRLLRESFAGHGGLFGEMVERQDRL
                     RTVALPARSANAGQPLESVMNDGIVVTALVREGVRQLNPDAQTLMAGEDILVLFGSPE
                     DLDRAERALLR"
     gene            1927..>2594
                     /locus_tag="PLW13_15940"
     CDS             1927..>2594
                     /locus_tag="PLW13_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71921.1"
                     /translation="MNPPALRDALALHRAGRLSEAEAGYRAIIAATADAEAMQLLASL
                     LHQDGRSHEALAWLDRALPQLALRDAAQSNRAAMLLALGRFDEAVASAEQVLARNPHH
                     AGATQNRRLALLGQARAGGELLAKARHYARYAAAGGDDPVAFLEFGNALQNIGAAQEA
                     IAAYERARELDPRSPEIASAALIAAHFGADADAAALAQRARAAAAVHARGVTARDAGG
                     RDAD"
BASE COUNT          340 a          893 c          928 g          433 t
ORIGIN      
        1 gcttccagcg cctgcgcatt ccgaccagcc tgggttacct gctggtcgga ttgatactcg
       61 ggccgcatac cgccggcccg gcgctgcaga ccgcgccgat ccaggcgctg gcggagttcg
      121 gcatcgtgtt cctgctgttc accgtggggc tgaactattc gctgccgcag atccacgcgc
      181 tgcgcaacac ggtgctcacg ctgggcacgg cgcaggtcgg gttgactacg ctcgtcgtcg
      241 gcctgctcgc gtatttcgtg ctgggcctgc cggcggcaac cgccttcgtg atcggcgccg
      301 tgttcgcgca gtcctccacc accgtcatca gcaaccagtt gatcgcccag ggcgaagaca
      361 acagccggca cggacgtctc ggcgtggcga tgtccgtgtt ccaggacgtg accgcggtgc
      421 cgctgctgat cctgatcccg gtgttcggcg ccgccgccgg attcaccacg gtgctcggtg
      481 acatcgcgct ggcgctggcc aaggccgtgc tggcgttcgt cgtggtgctg tacggcgggc
      541 gctggctgtt gcggccactg tttcattacg tggccgggca gcgctcgccc gaactgttca
      601 cgctgaccgt gctgctggtg tcgctcgccg cgggctgggc caccggcaag ctgggcctgt
      661 cgatggcctt tggcgccttc ctcgccggca tgatgctcgg cgaaaccgag ttcaggcacc
      721 aggtcgaggc cacgatccgt ccgttccgcg acgtgctgct cgggctcttt ttcgtcggcg
      781 tcggcatgct gatcgacccg atggtgctgc cgggaatctg gcactgggtg ctcatcggca
      841 ccgccgtgct gctgctcagc aagatcctgc tggtcgcgca gatcgtgcgc tggtccggcg
      901 tcgatccgct caccgcctgg agcaccgcgc tgctgctggc ggtcggcggc gagttcggtt
      961 tcgcggtgct gtcgatttcg ctcggcgatg gggccatcga cagccacacc gggcagatcg
     1021 tgctgtattc ggtgctgctg tcgatcatcg ttgcgccgtt cctgatccgc tacaaccacg
     1081 cgatcgcgac ctggctctcg cccgccgtga ccgcggcagt gaagccgggc gtgcccggcg
     1141 ccagcgcgga gcaggccggc aagctcgatg agcacgtcgt catctgcggc tatggccgca
     1201 tgggccagag cgtcggcaat ttcctggagg aggagggcat cccctacgtg gcgctggatc
     1261 tcgactcgga tcgcgtgcgc gaggcgcacc tcgcgggcga gccggtgttc tatggcgatg
     1321 ccagcgacgg cagcgtgctg gacgcactcg gcatcgcgcg ggcgcggctg ttggtgatca
     1381 gccacaacga catcgccgcc gcgcgccgca tcctgcatca cgtgcgccgc cagcgtcccg
     1441 agctgccggt gatggtgcgc accatcgacg agacgcatgt ggaggagttg cgcgcggcgg
     1501 gcgccaccga ggtcatcccc gagacgctcg aagccagcct gatggtggcg tccgccgcgc
     1561 tgctgctgct ggacgtgccg ctggtgcgcg tggtgcagaa aatgcgcgaa cagcgcaaca
     1621 gccactaccg gctgctgcgc gagtccttcg ccggccacgg cgggctcttc ggcgagatgg
     1681 tggagcggca ggatcgcctg cgcacggtgg cgctgcccgc gcgctcggcc aatgcgggac
     1741 agccgctgga atccgtcatg aacgatggca tcgtcgtcac cgcgctggtg cgcgagggcg
     1801 tgcggcagct gaaccccgat gcgcaaacgc tcatggcggg cgaggacatc ctggtgctgt
     1861 tcggatcgcc cgaggacctg gatcgcgcgg aacgcgcatt gctgcggtga gccggagcgc
     1921 tgttcaatga acccgcccgc gctgcgcgac gcgctggcgc tgcaccgcgc cggccggctg
     1981 tccgaggccg aggcgggcta tcgcgccatc atcgcggcca ccgccgacgc cgaggcgatg
     2041 caactgctgg cctcgctgct gcaccaggac ggtcgctcgc acgaagcgct ggcctggctg
     2101 gatcgcgcgc tgccgcaact ggcgctgcgc gatgcggcgc aatcgaatcg cgcggcgatg
     2161 ctgctggcgc tcgggcgctt cgacgaagcc gttgccagtg ccgagcaggt gctggcgcgc
     2221 aatccgcacc atgcgggcgc gacgcagaac cgccgcctcg cgttgctggg gcaggcgcgc
     2281 gcgggcggcg agctgctcgc gaaggcgcgc cactatgcgc gctatgccgc cgccggcggt
     2341 gacgatcccg tcgccttcct cgaattcggc aacgccctgc agaacatcgg cgccgcgcag
     2401 gaggcgatcg cagcctacga gcgcgcgcgt gaactcgatc cgcgctcgcc cgagatcgcg
     2461 agtgccgcgc tgatcgccgc acatttcggc gcggatgcgg atgccgccgc gctggcgcag
     2521 cgcgcgcggg ccgccgccgc cgtgcatgcg cgcggcgtga ccgcgcgcga tgcgggcggg
     2581 cgtgacgcgg actg
//
LOCUS       DAORUQ010000314         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_59911_length_2560_cov_4.796806, whole genome shotgun sequence.
ACCESSION   DAORUQ010000314 DAORUQ010000000
VERSION     DAORUQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59911_length_2560_cov_4.796806"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..698)
                     /gene="pgsA"
                     /locus_tag="PLW13_15945"
     CDS             complement(123..698)
                     /gene="pgsA"
                     /locus_tag="PLW13_15945"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQY71922.1"
                     /translation="MNIPNTLTLFRIALIPVFVILFYLPFSWSFLASALVFVLAAVTD
                     WLDGYLARRLDQSTSFGAFLDPVADKLMVVIAIVLLVTEQKTAWFTIPALVVVGREIL
                     VSALREWMAEIGKRTSVAVSWVGKVKTFVQMLAIITLLAVDPDKGGPWLALGYILFYA
                     AAALTLWSAVLYVRAAWGDLSDAATRPGRVN"
     gene            complement(732..2558)
                     /gene="uvrC"
                     /locus_tag="PLW13_15950"
     CDS             complement(732..2558)
                     /gene="uvrC"
                     /locus_tag="PLW13_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQY71923.1"
                     /translation="MTGFDAASFLRNLTELPGVYQMYDAAGTLLYVGKARNLRKRVSS
                     YFRGTGLGAKTEALVARIAEIQVTVTGSETGALLLEQNLIKSQHPPYNILLRDDKSYP
                     YIFMSQQETFPRLGFHRGAKRATGRYFGPFPSSTAVRESLNLLQKIFRVRQCEDSFFR
                     NRSRPCLQYQIGRCSGPCVGLVDEAEYAESVRHTAQFLEGRSSELAGELADAMERAAA
                     GLDFERAAVLRDQIRSLQAVREKQVIESGDGMVDVIAAAAGPGAACVHVLFVRDGRIL
                     GSKSFFPRCRLEESAAEVLSAFVAQFYLGASGEREIPREILLGEELADAEVLAEVLSD
                     ASGRKVALSARVRASRSQWLKLATQTAQQNLGGYLANRKRIHEQLGELQEALGLDSVP
                     ERIECFDISHSGGELAVASCVVFDAGGPLKSDYRRFNIEGITPGDDYAAMRQALERRY
                     TRLKSGEGKLPDLLLIDGGKGQMAQALEVLEELQIEGVTVTAVAKGTTRKPGLETLIV
                     GKARRELVLAPDSTALHLIQQVRDEAHRFAISALQKRRAKKSGGSSLEGIPGVGPRRR
                     RELLRYFGGIQGIERASLEELARVPGISRKIAEDVYATFHNQ"
BASE COUNT          432 a          881 c          825 g          422 t
ORIGIN      
        1 ggttcgaacc ggcgacctca accttggcaa ggttgcgctc taccaactga gctattcccg
       61 cgtgccgtga aggcctgcgt attctagagc gtgaacatca tgtgtcaaca ggaacgtgat
      121 gttcaattta cacgcccggg gcgggttgcc gcatccgaca gatcgcccca cgcggcccgc
      181 acgtagagca ccgctgacca cagcgtcagc gcggcggcgg catagaacag gatgtagccc
      241 agcgccagcc aggggccacc cttgtcggga tccaccgcga gcagcgtgat gatcgcgagc
      301 atctgcacga aggtcttgac cttgcccacc caggacaccg cgacgctggt gcgcttgccg
      361 atctcggcca tccattcgcg cagcgcggag accaggatct cgcgccccac caccacgagc
      421 gccgggatcg tgaaccaggc ggtcttctgc tcggtcacca gcagcacgat cgcgatcacg
      481 accatgagct tgtcggccac cggatcgagg aaggcgccga aagaggtcga ctggtcgagc
      541 cggcgcgcga gatagccgtc gagccagtcg gtcaccgcgg cgagcacgaa caccaacgcg
      601 gaggccagga agctccagct gaacggcagg tagaacagga tcacgaacac cgggatcagc
      661 gcgatgcgaa acagtgtcag cgtgttcggg atattcatcg atggctcacc gggctgcggt
      721 tttgattcat tctattgatt gtggaacgtc gcataaacat cctcggcgat ttttctgctg
      781 atgcccggca cgcgcgcgag ctcctccagg ctggcccgct cgatgccctg gatgccgccg
      841 aaatagcgca acagttcgcg ccgtcgccgc ggccccacgc cgggaatgcc ctcgagcgag
      901 gaaccaccgc tcttcttcgc gcgtcgcttc tgcagcgcgc tgatcgcgaa acggtgcgcc
      961 tcgtcgcgca cctgctggat caggtgcagc gcggtggagt ccggcgcgag caccagctcg
     1021 cggcgcgcct tgccgacgat cagcgtctcc aggcccggct tgcgcgtggt gcccttggcc
     1081 acggccgtta ccgtgacgcc ttcgatctgc agctcctcca gcacctccag cgcctgcgcc
     1141 atctgaccct tgccgccgtc gatcagcagc aggtcgggca gcttgccctc gcccgatttc
     1201 aggcgcgtgt agcggcgctc cagtgcctgg cgcatcgcgg cgtaatcgtc gcccggcgta
     1261 atgccctcga tattgaagcg tcgatagtcg gacttcagcg gtccaccggc atcgaacacc
     1321 acgcaggagg ccacggcgag ctcaccgccg ctgtggctga tgtcgaagca ctcgatgcgc
     1381 tcgggcacgc tgtccagtcc gagcgcctcc tgcagttcgc cgagctgttc gtggatgcgc
     1441 ttgcggttgg ccaggtatcc gcccaggttc tgttgcgcgg tctgggtcgc gagcttcagc
     1501 cactggctgc gcgaggcgcg cacgcgcgcg ctgagcgcga ccttgcgccc ggacgcgtcg
     1561 ctcagcactt cggccagcac ctcggcgtcg gccagctcct cgccgagcag gatctcgcgc
     1621 gggatctcgc gctcgccact ggccccgaga tagaactgcg ccacgaaggc cgacagcacc
     1681 tcggccgcgc tctcctccag ccggcagcgc gggaagaagc tcttgctgcc gaggatgcgc
     1741 ccgtcgcgca cgaacagcac gtgcacgcag gccgcacccg gacccgcggc cgcggcaatc
     1801 acgtcgacca tgccgtcgcc gctctcgatg acctgctttt cacgcaccgc ctgcaggctg
     1861 cggatctggt cgcgcaacac cgcggcacgc tcgaagtcga gcccggcggc ggcgcgctcc
     1921 atggcatcgg cgagctcgcc ggcgagctcg ctgctcctgc cctccaggaa ctgcgccgtg
     1981 tggcgcaccg actcggcgta ttccgcttca tccaccagcc ccacgcaggg gcccgagcag
     2041 cgcccgatct ggtattgcag gcaggggcgc gaacggttgc ggaagaaact gtcctcgcac
     2101 tggcgcacgc ggaagatctt ctgcagcagg ttgaggctct cgcgcactgc cgtggagctg
     2161 ggaaagggac cgaaatagcg ccccgtcgca cgcttggcgc cgcggtggaa accgagccgg
     2221 gggaaggtct cctgctgcga catgaaaata tacgggtacg acttgtcgtc gcgcagcagg
     2281 atgttgtacg gcgggtgctg gctcttgatc aggttctgct cgagcagcag cgccccggtt
     2341 tcgctgccgg tcacggtcac ctggatctcg gcaatgcgcg ccaccagcgc ctcggtcttg
     2401 gcgcccaggc cggtgccacg aaaatagctc gagacgcgct tgcgcaggtt gcgcgccttg
     2461 cccacgtaga gcagtgtgcc cgcagcgtcg tacatctggt agacgccggg cagctcggtg
     2521 agattgcgca ggaaggaggc tgcgtcgaag cccgtcacga
//
LOCUS       DAORUQ010000315         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_61857_length_2511_cov_4.881515, whole genome shotgun sequence.
ACCESSION   DAORUQ010000315 DAORUQ010000000
VERSION     DAORUQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61857_length_2511_cov_4.881515"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1262
                     /locus_tag="PLW13_15955"
     CDS             168..1262
                     /locus_tag="PLW13_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HQY71924.1"
                     /translation="MKTFTCCCGNTLHFENTRCLGCGRGVAFLPDALVVAALEPLGDR
                     GLRALLPWQWDMRYRRCRNDTDYQVCNWMVRDDDADAYCVSCRLNRVIPDLSSARNVE
                     LWRRIEEAKRRLMYTVLRLGLPFTGRDHDAHGGLGFRFLADTDADSEYSFHIPPRGRV
                     LTGHDDGLITINLAEADTVAREAMRERMNEEYRTLLGHFRHESGHYFWDRLLRDSALL
                     ADFRALFGDERADYALALSRHYAGGAPERWRDCWISAYAASHPWEDWAETWAHYLHMI
                     DTLETAEDFGFTIHGEPVRCSGSPALAGQPREDDFERLFDDWVRLTTAMNAINRSMGL
                     SDAYPFALNPPAARKLGFVHALVRCAREPE"
     gene            complement(1267..1998)
                     /locus_tag="PLW13_15960"
     CDS             complement(1267..1998)
                     /locus_tag="PLW13_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HQY71925.1"
                     /translation="MTYCLAIKVKDGLVFASDSRTSAGVDYVSVYSKMHTFQLTEGRV
                     FVLLSAGNLATSQAVVNSLRRDLADPEAPISLNSVHFPFDAAHYIGEISVRVQNEHPE
                     AMKKSGVNVEASFILGGQIGLGEHEIYLIYPQGNYISTSDETPYLQVGETKYGKPILD
                     RIVTPQTSLEDAARCALVSEDSTMRSNISVGPPLELLIYRADSLSIDTRLLLKPQSPL
                     YKKLQKSWNEGLRHVFDRLPRFDWE"
     gene            2131..>2511
                     /locus_tag="PLW13_15965"
     CDS             2131..>2511
                     /locus_tag="PLW13_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQY71926.1"
                     /translation="MSISWRDYPVAGLHDELIGARGRVREPARRLCHHLRNLDDADLH
                     ERRAAAEAAIQVMGITFTVYSEQEGSIDRAWPFDIIPRVIARAEWQRIEAGLRQRVRA
                     LNRFIDDVYHEQRIVKDGVFPAEVL"
BASE COUNT          400 a          816 c          850 g          445 t
ORIGIN      
        1 gcgacaggta gcctacaggg atgtatttac ggcgtgtccg cggcaccgat ggatgctcga
       61 gcgccgatgg ctcgcgatac gcgggattgt cgggctcgtt atcccaagca cagatggttt
      121 tgcggcatcc tctgaagccc gacccacgga gattttctgc atcgcacatg aagactttca
      181 cctgctgctg tggcaatacg ctgcacttcg agaacacgcg ctgcctcggc tgcgggcgcg
      241 gtgtggcgtt cctgcccgat gcgctggtcg tcgcggcgct ggaaccgctg ggcgaccggg
      301 ggttgcgcgc gctcctgccc tggcagtggg acatgcgcta ccgccggtgc cgcaacgaca
      361 ccgactacca ggtctgcaac tggatggtgc gcgacgacga cgccgatgcc tactgcgtct
      421 cgtgccgcct caatcgcgtg attcccgacc tgtcctcggc gcgcaatgtg gagctctggc
      481 gtcgcatcga ggaggcgaag cggcgcctga tgtacaccgt gctgcgcctc gggctgccct
      541 tcacggggcg cgaccacgac gcgcacggcg ggctcggctt ccgcttcctc gccgatacgg
      601 atgccgacag cgaatattca ttccacatcc cgccgcgcgg gcgtgtcctc accgggcacg
      661 acgacgggct gatcacgatc aacctcgccg aggccgacac ggtcgcgcgc gaagcgatgc
      721 gcgagcggat gaacgaggaa taccgcacgt tgctcgggca tttccgccac gagagcgggc
      781 actacttctg ggatcggctg ctgcgcgaca gtgccctgct cgcggacttc cgcgcgctgt
      841 tcggcgacga gcgcgcggat tacgcgctgg cgctgtcgcg tcattacgct ggcggcgcgc
      901 ccgagcgctg gcgtgactgc tggatcagcg cctacgcggc atcgcacccg tgggaggact
      961 gggccgagac ctgggcgcac tacctgcaca tgatcgatac gctggagacc gcggaggact
     1021 tcggcttcac cattcacggc gagcctgtgc gttgctccgg atccccggcg ctcgcgggcc
     1081 agccgcgcga ggacgatttc gagcgcctgt tcgatgactg ggtccggctc acgacggcga
     1141 tgaacgcgat caaccgcagc atgggtctca gcgacgccta tccgttcgcg ctgaacccgc
     1201 ctgccgcgcg caagctcggc ttcgtgcacg cactggttcg gtgcgcgcgt gaaccagaat
     1261 gagtcactac tcccagtcga agcgcggcag gcggtcgaag acgtgacgca ggccctcgtt
     1321 ccaggacttc tgcagcttct tgtacagggg tgactggggt ttgagcagca gccgcgtgtc
     1381 gatgctcagg ctgtcggcgc ggtagatcag cagttccagc ggcgggccca ccgagatgtt
     1441 cgagcgcatc gtcgaatctt ccgagaccag cgcgcagcgc gccgcgtctt ccagcgaggt
     1501 ctgcggcgtg acgatgcgat cgaggatggg cttgccgtac ttggtctccc cgacctgcag
     1561 gtagggcgtc tcgtccgagg tggaaatgta gtttccctgc gggtaaatga ggtagatctc
     1621 gtgttcgccg agcccgatct gcccgcccag gatgaagctc gcctcgacgt tcacgccgct
     1681 ctttttcatc gcctcggggt gctcgttctg cacccgcacg ctgatctcgc cgatgtagtg
     1741 cgccgcgtcg aaggggaaat gcaccgaatt gaggctgatc ggtgcctcgg gatcggccag
     1801 gtcgcggcgc agcgagttca ccaccgcctg gctggtggcg aggttgccgg ccgacagcag
     1861 gacgaagaca cgcccttctg tgagctggaa ggtatgcatt ttgctgtata cgctgacata
     1921 gtccacgccc gcactggtgc gtgaatccga ggcgaacacc agtccgtcct tgaccttgat
     1981 cgcgagacag taagtcatgc agaaccagtt ccttgatgcc gtggtccggg cgggcccgcc
     2041 gggccggcgc cgattgtggc ggcgaatcgc gaatcaatcc agcacattcg gcgcccgcgc
     2101 gcgggcgccg cgtgccacgg tggaggcagc atgagcattt cctggcgcga ctacccggtg
     2161 gcgggcctgc acgacgaact gatcggagcg cgcgggcgtg tgcgcgagcc ggcgcgacgc
     2221 ctgtgccatc acctgcgcaa cctcgacgac gcggacctgc acgagcgccg tgcggccgcc
     2281 gaggcggcga tccaggtcat gggcatcacc ttcacggtgt attcggagca ggagggctcg
     2341 atcgaccgcg cctggccctt cgacatcatc ccgcgcgtga tcgcgcgcgc ggagtggcag
     2401 cgcatcgagg cggggctgcg ccagcgcgtg cgcgcgctga atcgcttcat cgacgatgtc
     2461 taccacgaac agcgcatcgt gaaggacggc gtgttcccgg ccgaggtgct g
//
LOCUS       DAORUQ010000316         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_62045_length_2506_cov_7.283966, whole genome shotgun sequence.
ACCESSION   DAORUQ010000316 DAORUQ010000000
VERSION     DAORUQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62045_length_2506_cov_7.283966"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="PLW13_15970"
     CDS             <1..1634
                     /locus_tag="PLW13_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71927.1"
                     /translation="EAAIAARVGEMLRRFGLDKVRGNLPERLPLGMRQRLSLAVALVH
                     GPELLILDEPTSGVDPVARDEFWRLLVELSREDGVTIFISTHFMNEAERCDRISLMHA
                     GRVLVSDAPGELVRQRDAATLEQAFIAWLREASVDDAAVAETPATAAPAAPAADAAPR
                     RARRRRAFDWQRALGYTRREALELRRDPVRGLMALLGSVILMFIMGYGISMDVEDLRY
                     AVLDQDQSALSQSYTLNLSGSRYFIEQPPLASYADIDARMRSGDITLAIEIPPGFARD
                     AMHGRPVQIGAWVDGSMPVRAETVRGYVQGMHQGWLLQMASTRAGANASALAAPARVE
                     TRFRYNPDVRSLPAMVPAVIPLLLVMIPAMLTALSVVREKELGSIINLYVTPVSRAEF
                     LLGKQLPYVVLSMLNFLLLSALAVTVFGVPIKGSFATLALGALLFSLCTTGLGLVAST
                     VTRSQVAAIFFTLIATLLPAIQFGGLLNPVSSLEGLGRFIGETYPVSHFLVISRGVFS
                     KALDLPRLAGSFWPIVIAVPIILGIAIALLKKQER"
     gene            1636..>2506
                     /locus_tag="PLW13_15975"
     CDS             1636..>2506
                     /locus_tag="PLW13_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY71928.1"
                     /translation="MPRLANIFRLGVKELWSLARDPIMLGLIAYAFTLSIYSAATAMP
                     ETLHMAPIAIVDEDGSPLSSRISASFYPPRFLAPALISLDAIDPGMDAGHYTFVLVIP
                     VDFQRDVLAGRSPQIQLNVDATRMSQAFTGSGYIQQIVMGEVSEYVKRYRETTALPVD
                     LALRMRFNQNLEPSWFGALGEIVNRITMLSMVLTGAALIREREHGTIEHLLVMPVTPA
                     EIMLSKVWSMGLVVLGAAALSLAFVVQGAMRVPVEGSVALFMTLAAVHLFATTSMGVF
                     MATLARTMPQFALL"
BASE COUNT          350 a          828 c          877 g          451 t
ORIGIN      
        1 cggaggcggc gatcgcggca cgggtcgggg agatgctgcg gcgcttcggc ctcgacaagg
       61 tgcgcggcaa cctgcccgag cggctgccgc tgggcatgcg ccagcgcctg tcgctggccg
      121 tggcgctggt gcacgggccc gaattgctga tcctggacga gccgacctcg ggcgtcgatc
      181 cggtggcgcg cgacgaattc tggcgcctgc tggtcgagct ctcgcgcgag gacggcgtga
      241 ccatcttcat cagcacgcat ttcatgaacg aggccgagcg ctgcgaccgc atctcgctga
      301 tgcacgccgg gcgcgtgctc gtgagtgacg caccgggcga actggtgaga caacgcgatg
      361 ccgccacgct cgaacaggct ttcatcgcct ggctgcgcga ggcgagtgtc gatgacgctg
      421 ctgtcgcaga gacgcctgcc actgccgcac ctgccgcacc tgccgccgat gccgcgccgc
      481 gccgtgcccg gcgccggcgg gcgttcgact ggcagcgggc cctcggctat acgcggcgcg
      541 aggcgctgga gctgcggcgc gatccggtgc gcgggctgat ggcgctgctc ggttcggtca
      601 ttctgatgtt catcatgggc tacggcatca gcatggacgt ggaggatctg cgctacgcgg
      661 tgctcgatca ggaccagtcc gcgctcagcc agagctatac gctgaacctc tcgggctcgc
      721 gttatttcat cgagcagccg ccgctcgcca gctacgccga catcgatgcg cgcatgcgca
      781 gcggtgacat cacgctcgcc atcgagatac cgccgggttt cgcgcgcgat gcgatgcacg
      841 gcaggccggt gcagatcggc gcctgggtcg atggctcgat gccggtgcgc gccgagaccg
      901 tgcgcggcta tgtgcagggc atgcaccagg gctggctgct gcagatggcg agcacgcgcg
      961 ccggcgcgaa cgccagcgca ctggccgcgc cggcgcgcgt cgagacgcgc tttcgctaca
     1021 acccggatgt gcgcagcctg ccggcgatgg tgccggcggt gatcccgctc ctgctggtca
     1081 tgatcccggc gatgctgacc gcgctgtcgg tggtgcgcga gaaggagctc ggctcgatca
     1141 tcaatctcta cgtcacgccg gtgagccgcg ccgagttcct gctgggcaag cagctgccct
     1201 acgtcgtgct gtcgatgctg aatttcctgc tgctgagcgc gctcgcggtg acggtgttcg
     1261 gggtgccgat caagggcagc tttgcgacgc tggcgctggg agcgctgctg ttctcgctgt
     1321 gtaccaccgg cctcggtctg gtggcctcga cggtcacgcg cagccaggtc gcggcgatct
     1381 tcttcacgct catcgcgacg ctgctgcccg cgatacagtt cggcggcctg ctcaacccgg
     1441 tgtcttcgct ggaggggctg ggcaggttca tcggcgagac ctacccggtc tcgcactttc
     1501 tcgtcatcag ccgcggcgtg ttcagcaagg ccctggatct gccgcgcctc gcaggctcgt
     1561 tctggccgat cgtgatcgcc gtgccgatca tcctcggcat tgccatcgcg ctgctgaaga
     1621 agcaggagcg ctgagatgcc gcggctggcc aatatcttcc ggctgggcgt caaggaactg
     1681 tggagcctgg cgcgcgaccc gatcatgctg gggctcatcg cctacgcgtt cacgctgtcg
     1741 atctacagcg ccgcaacggc gatgcccgag acgctgcaca tggcgccgat cgcgatcgtc
     1801 gacgaggacg gctcgccgct ctcctcgcgc atcagcgcga gcttctaccc gccacgcttc
     1861 ctcgcgccgg cgctgatctc cctggacgcg atagaccccg ggatggatgc cgggcactac
     1921 accttcgtgc tggtgattcc ggtggatttc cagcgcgacg tgctggccgg ccgttccccg
     1981 cagatccagc tgaatgtcga tgccacccgc atgagccagg cgttcaccgg cagcggctac
     2041 atccagcaga tcgtgatggg cgaggtgagc gagtacgtga agcgctatcg cgagacgacc
     2101 gcgctgccgg tggacctcgc tctcaggatg cgtttcaacc agaacctgga gccatcgtgg
     2161 ttcggcgcgc tgggcgagat cgtcaaccgg atcacgatgc tgtcgatggt gctcaccggc
     2221 gcggcgctga tccgcgagcg cgagcacggc accatcgagc acctgctggt catgccggtg
     2281 acgccggccg agatcatgct gtccaaggtc tggtcgatgg ggctggtggt gctgggcgcg
     2341 gcggccctgt cgctcgcctt cgtggtgcag ggggcgatgc gggtgccggt cgagggctcg
     2401 gtggcgctgt tcatgacgct ggcagcggta catcttttcg cgaccacctc gatgggggtg
     2461 ttcatggcga cgctggcgcg cacgatgccg cagttcgccc tgctgc
//
LOCUS       DAORUQ010000317         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_62181_length_2503_cov_4.109069, whole genome shotgun sequence.
ACCESSION   DAORUQ010000317 DAORUQ010000000
VERSION     DAORUQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62181_length_2503_cov_4.109069"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /gene="rph"
                     /locus_tag="PLW13_15980"
     CDS             complement(<1..163)
                     /gene="rph"
                     /locus_tag="PLW13_15980"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939680.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQY71929.1"
                     /translation="MSIRPSGRAPDALRPVRITRGFTRHAEGSVLVEFGDTRVLCTAS
                     VEDKVPPFLR"
     gene            complement(176..1084)
                     /locus_tag="PLW13_15985"
     CDS             complement(176..1084)
                     /locus_tag="PLW13_15985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HQY71930.1"
                     /translation="MRFSIYQDSLIGARASNQDRMGYCYTRDSLLMLVADGMGGHMRG
                     EVAAHLSLQVAAATFQEDARPILADPSAFLERAMRRAHREILRYQDTHGLPESPRTTI
                     VACVVQGGRAWWAHAGDSRLYLLRDGRVLMRTRDHSKVQSLIDLGLIDERDSCTHPER
                     NKVMNCLGSPFEPTIELGGDVALEPGDTLMLCSDGVWSALPEDALIEPLTVESVLSGV
                     PRLVQQAVGHAGPMADNATVLAMTWESDVEGAVRSSQDVPEGAITTTIAVGMIEENAP
                     GETLTEDEIERTIREIREAISRGGAH"
     gene            complement(1107..2111)
                     /locus_tag="PLW13_15990"
     CDS             complement(1107..2111)
                     /locus_tag="PLW13_15990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQY71931.1"
                     /translation="MGTQTNAPLPAGHELGGYRIVRKIASGGFSIVYLAEDAAGEKYA
                     VKEYLPSALVKRSAGQLVPDIPAASEATFRNGLRCFLEEGRSLGPIFHPNVVRVLNFF
                     RANETVCMVMAWERGRSLQEVVLRHRGGAPGKEVLPERHVLRVFNLAMNGLREVHANR
                     LLHLDLKPANIYLRLDGTPLLLDFGAARRALDAEPQKLFPMYTPGFAAPELYRRTQQL
                     GPWTDVYGVGASMYGCLCGQPPQPADQRELEDKVPEAIEALAGAYSRPLLDLVGWCLK
                     LNPLERPQSVFALQRALRRIPVPAESAARPAAAGRWFDSLVARAGIRRGDGESLTLPG
                     "
     gene            2190..>2503
                     /locus_tag="PLW13_15995"
     CDS             2190..>2503
                     /locus_tag="PLW13_15995"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71932.1"
                     /translation="MTQRTAERRPLQSMTGYAQVSRDTPAGQLSVELRSVNSRFLDLS
                     LRMPDELRVAEPALRELIVAAVQRGKVECRVGVRTLARDDAAPQVDGHVLAQLAELVR
                     RVA"
BASE COUNT          382 a          893 c          846 g          382 t
ORIGIN      
        1 cgcgcaggaa cggcggcacc ttgtcctcga cgctggcggt gcacagcacc cgcgtgtcgc
       61 cgaactcgac cagcaccgag ccttcggcgt gccgggtgaa accacgggtg atgcgcaccg
      121 ggcgcaatgc atcgggcgcg cgcccgctgg gacgaatcga catcgaagac tcctttcagt
      181 gcgccccgcc gcgcgagatc gcctcgcgga tctcgcggat ggtgcgctcg atctcgtcct
      241 cggtcagggt ctcgcccggc gcattctcct cgatcatgcc gaccgcgatg gtggtggtga
      301 tggcgccttc ggggacgtcc tgcgacgacc ggaccgcgcc ttcgacgtcg ctctcccagg
      361 tcatcgccag cacggtggcg ttgtcggcca tcggaccggc gtgcccgacc gcctgctgca
      421 ccagccgcgg cacgccgctg agcaccgact cgacggtcag cggctcgatc agggcatcct
      481 cgggcaacgc cgaccacacg ccatcgctgc acagcatcag cgtgtcgccg ggctcgagcg
      541 ccacgtcgcc gcccagctcg atggtcggct cgaacggcga accgaggcaa ttcatcacct
      601 tgttgcgctc cgggtgcgtg cagctgtcgc gctcgtcgat caggccgagg tcgatcaggc
      661 tctgcacctt cgagtgatcg cgcgtgcgca tcagcacgcg cccgtcgcgc agcaggtaca
      721 gccgcgagtc gccggcatgc gcccaccagg cgcgcccgcc ctgcacgacg caggcgacga
      781 tggtggtgcg cggcgattcc ggcagcccgt gcgtgtcctg gtagcgcagg atctcgcggt
      841 gcgcgcgccg catcgctcgc tcgagaaaag ccgacggatc ggccaggatc ggccgcgcgt
      901 cttcctggaa ggtggcggcg gcgacctgca gcgacaggtg ggcggcgacc tcgccgcgca
      961 tgtgtccacc catgccgtcg gcgaccagca tcagcaggct gtcgcgcgtg tagcagtacc
     1021 ccatccggtc ctggttggag gcgcgcgcgc cgatcaggct gtcctggtag atcgaaaaac
     1081 gcatggcgac ctgcgatgtc gcccgctcaa cccggcagcg tcaggctttc gccgtcaccg
     1141 cgccggatgc ctgcgcgcgc cacgagcgag tcgaaccaac ggcccgcggc ggccggcctg
     1201 gcagcggatt cggccggtac cgggatgcgc cgcaacgcgc gctgcagcgc gaacacgctc
     1261 tgcggccgct cgagcggatt gagcttcagg caccaaccga ccaggtcgag caacggacgc
     1321 gaatacgcgc cagccagcgc ctcgatcgcc tcggggacct tgtcttccag ctcgcgctgg
     1381 tcggccggct gcggcggctg cccgcacagg cagccgtaca tgctcgcacc gactccgtag
     1441 acgtccgtcc acggccccag ctgctgcgtg cgccgataca gctcgggcgc ggcgaaaccg
     1501 ggcgtgtaca tcggaaacag cttttgcggc tcggcatcga gtgcgcgccg cgcggcgccg
     1561 aagtcgagca gcagcggcgt accgtcgagc cgaaggtaga tgttggcggg cttcaggtcg
     1621 aggtgcagca gacggttggc gtgcacctcg cgcagcccgt tcatcgccag gttgaacacg
     1681 cgcaacacgt gccgctcggg caggacttcc ttgcccggtg cgccgccgcg atgacgcagg
     1741 accacctcct gcaggctgcg accgcgctcc caggccatca ccatgcagac ggtctcgttg
     1801 gcgcggaaga agttgagcac ccgcaccacg ttcggatgga agatgggccc cagcgagcgg
     1861 ccttcctcga ggaagcagcg cagaccgttg cggaaggtgg cctcgctggc ggccggaatg
     1921 tccggcacca gctgaccggc gctgcgcttg accagcgcgc tcggcaggta ttccttgacc
     1981 gcgtacttct cgccggcggc atcctcggcc aggtagacga tcgagaatcc gccgctcgcg
     2041 atcttcctta caatgcggta tccgccaagc tcgtgcccgg caggcagcgg tgcattcgtc
     2101 tgtgtaccca tcgcgtcgga agatccgtcc tggctgtcgg tgatggcgtt tggcgcattc
     2161 taaagacttc cagcctgcga agaccgatct tgacccaacg aaccgccgaa cggcgccccc
     2221 tgcagagcat gaccggctac gcgcaggttt cgcgcgacac tcccgccggg cagctcagcg
     2281 tcgaactgcg cagcgtcaac tcgcgcttcc tcgacttgtc gttgcgcatg cccgacgaac
     2341 tgcgcgtggc cgaaccggcg ttgcgcgagc tgatcgtcgc cgcggtccag cgcggcaagg
     2401 tcgagtgccg ggtcggcgtg cgcacgctgg cccgcgacga cgcggcgccg caggtcgacg
     2461 gccacgtgct ggcgcagctc gccgagctcg ttcgccgggt ggc
//
LOCUS       DAORUQ010000318         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_64438_length_2449_cov_4.487051, whole genome shotgun sequence.
ACCESSION   DAORUQ010000318 DAORUQ010000000
VERSION     DAORUQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64438_length_2449_cov_4.487051"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLW13_16000"
     CDS             complement(<1..704)
                     /locus_tag="PLW13_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71933.1"
                     /translation="MLRAICFSLLAASMALTATRSSAAEPAAPEQRPTYGVLYTAWIK
                     AGEPWATVRIRLTRHPEWVRWMRFEIDPARYKGFKGEGSIAQEANTVLWKPPAEDAWI
                     QYKVLLTSQRESGRYDGAITDTWALFRAEDLVPVKRIDFQDGTQSQAKLRLNVPEGWS
                     IESAHPRYRGGRLKIDDPRGNFDRPTGWLLLGDLGVRREKIGNTRVAVGAPTGQGLRR
                     MDIMAFFRWTLPTLQKV"
     gene            905..1846
                     /locus_tag="PLW13_16005"
     CDS             905..1846
                     /locus_tag="PLW13_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY71934.1"
                     /translation="MIEVTQLGKSFGSLGAVRGIDFSVRPGEVLGFLGPNGAGKSTTM
                     KMIAGYLAPTTGSVRVCGCDVQEDTLAARRLIGYLPEGAPAYDDMTVIEFLRFIAEVR
                     GFAGAAREERVGRALEQMDLGAVCHQRIETLSKGFKRRVGLAQAILHDPQVLILDEPT
                     DGLDPNQKHQVRELIRGLSGEKIVIISTHILEEVHAVCNRAIVIAAGRIVADGTPASL
                     EARSRYHRSVHIHFGADMRVVDAFEAREALGALPGVAEVESHANGRRLVVVAVPGSNP
                     LPSVAHLVHDRGWEIDELHVERGRLDEVFRDITREVA"
     gene            1843..>2449
                     /locus_tag="PLW13_16010"
     CDS             1843..>2449
                     /locus_tag="PLW13_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY71935.1"
                     /translation="MSAMTGIIARRELAAYFATPLAYVFVLIFLMLGGAFTFYIGGFF
                     ERGQADLAPFFNFHPWLYLFLVPAVAMRLWSEERKSGTIELLLTLPVTRWQAVLGKFL
                     AAWLFTGLALVLTFPLWITVNVLGSPDNGVILSSYVGSWLMAGSFLAIGSCMSALTRN
                     QVIAFILTVVVCFLFILSGFPLVLDAVSAWAPRVLVDAIASM"
BASE COUNT          348 a          819 c          861 g          421 t
ORIGIN      
        1 accttctgca gcgtcggcag ggtccagcgg aagaacgcca tgatgtccat gcgccgcagg
       61 ccctggcccg tgggcgcgcc gaccgcgacg cgggtattgc cgatcttctc gcggcgcacg
      121 ccgaggtcgc cgagcagcag ccagccggtg gggcggtcga aattgccgcg cggatcgtcg
      181 atcttcagcc ggccgccgcg atagcgcgga tgcgccgatt cgatcgacca gccctcgggg
      241 acgttgaggc gcagtttcgc ctgcgactgg gtgccgtcct ggaaatcgat gcgcttgacc
      301 ggcaccaggt cctcggcacg gaacagcgcc cacgtgtcgg tgatcgcgcc gtcgtagcgc
      361 ccggactcgc gctggctggt gagcagcacc ttgtactgga tccaggcgtc ctcggccggc
      421 ggcttccaca gtaccgtgtt ggcctcctgc gcgatgctgc cttcgccctt gaaacccttg
      481 tagcgcgccg ggtcgatctc gaagcgcatc cagcgcaccc actcggggtg gcgcgtgagg
      541 cggatgcgca ccgtggccca cggctcgccg gccttgatcc aggccgtgta cagcacgccg
      601 tacgtcgggc gctgctccgg cgcagccggc tcggcggccg acgatctggt ggccgtcaga
      661 gccatgctcg ccgccagcag gctgaaacag atcgcgcgca gcatcgaact tcctccaggg
      721 gatcgggtcg aagcgccgcg tgcgcagtcg cgccactgcg gccccgggct cactatagtg
      781 agcgccattg cgcgcaacaa tgaattagtc ccgcgccgcg ttggcacggg cggttgctgg
      841 agggcagggg catgggtata gttcggcgcc atttgaaatc ggcagggaca gcaggagcgc
      901 gggcatgatc gaagtcacgc agctcggcaa gagcttcggg tcactgggcg cggtgcgcgg
      961 catcgacttc agcgtgcgcc ccggcgaggt actgggcttc ctcggcccga acggcgccgg
     1021 caagtccacc accatgaaga tgatcgcggg ttacctcgcg cccaccaccg gcagcgtgcg
     1081 cgtatgcggc tgcgatgtgc aggaggacac gctcgccgcg cggcgcctga tcggttatct
     1141 gcccgagggc gcgccggcct acgatgacat gacggtcatc gagttcctgc gcttcatcgc
     1201 cgaggtgcgc ggcttcgcgg gcgctgcgcg cgaggagcgc gtggggcgcg cgctggagca
     1261 gatggatctc ggtgcggtgt gccaccagcg catcgagacg ctgtcgaagg gcttcaagcg
     1321 ccgcgtgggg ctggcgcagg cgatccttca cgatccacag gtgctgatcc tcgacgagcc
     1381 caccgacggg ctcgatccga accagaagca ccaggtgcgc gaattgatcc gcgggctgtc
     1441 cggcgagaag atcgtgatca tctccacgca catcctcgag gaggtgcacg cggtgtgcaa
     1501 ccgcgcgatc gtcatcgccg ccgggcgcat cgtggccgac ggcacgccgg cctcgctcga
     1561 ggcccgctcg cgctaccacc gctcggtgca catccatttc ggggccgaca tgcgcgtcgt
     1621 cgacgccttc gaggcgcgcg aggcgctcgg cgcgctgccg ggcgtggccg aggtggaatc
     1681 gcacgccaac gggcggcgcc tggtggtggt cgcggtgccg ggcagcaacc cgctgccctc
     1741 ggtcgcgcac ctggtgcacg acaggggctg ggaaatcgac gagctgcacg tcgagcgcgg
     1801 gcggctcgac gaagtgttcc gggacatcac gcgggaggtg gcatgagcgc catgaccggc
     1861 atcatcgcgc ggcgcgagct ggcggcctat ttcgccacgc cgctcgccta cgtgttcgtg
     1921 ctcatcttcc tgatgctggg cggcgcgttc acgttctaca tcggcggctt cttcgagcgt
     1981 ggccaggccg atctcgcgcc gttcttcaat tttcacccct ggctctacct gttcctggtg
     2041 cctgcggtcg ccatgcgcct gtggtccgag gagcgcaaga gcggcaccat cgaactgctg
     2101 ctgacgctgc cggtgacgcg ctggcaggcg gtgctgggca agttcctggc ggcgtggctg
     2161 ttcaccggcc tggcgctggt gctcaccttc cccttgtgga tcaccgtgaa cgtgctcggc
     2221 agccccgaca acggcgtgat cctgtcgagc tacgtcggca gctggctgat ggccggcagt
     2281 ttcctcgcga tcggttcgtg catgtcggcg ctgacgcgca accaggtgat cgccttcatc
     2341 ctcaccgtgg tggtgtgctt cctgttcata ctgagcggct ttccgctggt gctcgacgcg
     2401 gtgagcgcct gggcgccgcg cgtgctggtc gacgcgatcg cctcgatga
//
LOCUS       DAORUQ010000319         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_64563_length_2446_cov_5.202426, whole genome shotgun sequence.
ACCESSION   DAORUQ010000319 DAORUQ010000000
VERSION     DAORUQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64563_length_2446_cov_5.202426"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLW13_16015"
     CDS             complement(<1..580)
                     /locus_tag="PLW13_16015"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71936.1"
                     /translation="MLDMIIRGGSVIDGSGAPARLADVGIRDGRIAAIGTLTEAAREE
                     IDATGLTVAPGFVDVHTHYDAQAFWDRTLSPSCYHGVTTVIAGNCGFSIAPLNGKPED
                     SEYLMRMLARVEGMPLESLREGVPWNWRSFGEYLDALDGKLAINAGFMVGHSALRRHV
                     MGARAVGSHATGAEIAAMQQLLRESIAAGGLGF"
     gene            754..1362
                     /locus_tag="PLW13_16020"
     CDS             754..1362
                     /locus_tag="PLW13_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY71937.1"
                     /translation="MKPTAERVLDVAEDLFAEKGYQAASLGDVAERVGIRSPSLYNHF
                     RNKEALYHAVLARLLEKFNAPLRELQEGEATPERIRGWQARLVRMHIDNPNLARLLQH
                     EALSGGPGSELIIEQLFKPLIESSSSAMDSGVAQSPSASRLRPWIVMGFNNIVMSYVT
                     MAPLYRGLLDIDPLGAEAAERQVEFITRLTDAFWMSGLGKEF"
     gene            1368..2372
                     /locus_tag="PLW13_16025"
     CDS             1368..2372
                     /locus_tag="PLW13_16025"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQY71938.1"
                     /translation="MSKAFPIHVEQTPEGDYLLSWTEEFSDAGVEVRAHSAPDAAPGA
                     EPVARAARPGVLIAGLEGPRHYFHLQPEQGEGLTAAQRNVPLQAGTNFRDMGGYRGAD
                     GRRVRWGRLFRSGHTAALSERDQEVVAALDIRVCCDFRREDEHVIEPTRLPPSTRIVG
                     LTVDPGSTSSFFQQVAERGAGPADMAAFMEEINREFVRHHAPQFRRMLGELLALEDGG
                     FMINCAAGKDRTGFGAAMILAALGVAEDDIVADYLLSDRYFPIEREMDRVLRKYGGEA
                     GGRLDPQLIRPMMETRREYIEAALEAIRRDYGSLERFLDEAMGVGPAERRELRERFTA
                     "
BASE COUNT          379 a          823 c          857 g          387 t
ORIGIN      
        1 agaagcccag cccgccggcg gcgatcgatt cgcgcagcag ttgctgcatc gcggcgatct
       61 cggccccggt ggcgtggctg ccgaccgcgc gcgcgcccat cacgtggcgg cgcaacgcgg
      121 agtgcccgac catgaagccg gcgttgatcg cgagcttgcc gtcgagcgcg tcgaggtact
      181 cgccgaagct gcgccagttc catggcacgc cctcgcgcag gctctccagt ggcatgccct
      241 cgacgcgcgc cagcatgcgc atcaggtact cgctgtcctc gggcttgccg ttgagcggcg
      301 cgatgctgaa accgcagttg cccgcgataa cggtggtcac gccgtgatag cacgaggggc
      361 tcagcgtgcg gtcccagaaa gcctgcgcgt cgtaatgcgt gtgcacgtcg acgaagcccg
      421 gcgcgaccgt gaggcccgtg gcgtcgattt cctcgcgcgc cgcctccgtg agagtgccga
      481 tcgcggcgat gcgcccgtcg cggatgccta cgtcggcgag gcgcgcgggc gctccgctgc
      541 cgtcgatgac gctgccgccc ctgatgatca tgtccagcat gctgtgggtt ctccgtcggg
      601 tactcttgtc gggcgcccat cttttccccc cgccctgacc aagtcaatca agccaacatg
      661 acagttgatc aagaaatgag tatgggctat tggtcgttag tcgggtgcta tactcgcgcc
      721 gcttcaacga tccgggctgc acggggtgcc ttgatgaaac caaccgccga gcgcgtgctc
      781 gacgtggccg aagacctgtt cgcggagaag ggctaccagg cggcatcgct cggcgacgtg
      841 gcggagcgcg tcggcatccg ttcgcccagc ctgtacaatc acttccgcaa caaggaggcg
      901 ctgtatcacg cggtgctggc gcggctgctg gagaagttca acgcgccgct gcgcgagttg
      961 caggagggcg aggccacgcc cgagcgcatc cggggctggc aggcgcggct ggtgcgcatg
     1021 cacatcgata accccaacct cgcgcgcctg ctgcagcacg aggcgctctc cggaggcccc
     1081 ggttcggagc tgatcatcga gcagctgttc aagccgctga tcgagagcag ttcgagcgcg
     1141 atggattccg gtgtcgcgca gagtcccagc gcctcgcgcc tgcgcccgtg gatcgtgatg
     1201 ggcttcaaca atatcgtgat gtcctacgtc accatggcgc cgctgtaccg cgggctcctg
     1261 gacatcgacc cgctgggcgc cgaggccgcg gaacggcagg tcgaattcat cacgcggctg
     1321 accgatgcat tctggatgag cggactgggc aaggaattct agacacgatg agcaaggcat
     1381 ttccgataca cgtggagcag actcccgagg gcgactacct cctgagctgg accgaggagt
     1441 tcagcgacgc gggcgtggaa gtccgcgccc actccgcgcc cgatgcggcg cccggggccg
     1501 agccggtggc gcgcgcggcg cgtcccggcg tgctcatcgc ggggctcgag gggccgcggc
     1561 attatttcca cctgcagccc gagcagggcg agggcctcac cgccgcgcaa cgcaacgtgc
     1621 cgctgcaagc cggcaccaat ttccgcgaca tgggcggcta ccgcggcgcc gacggccggc
     1681 gcgtgcgctg gggccggctg tttcgctccg ggcacacggc ggccctgagc gagcgcgacc
     1741 aggaagtcgt ggcggcgctg gacatccgtg tctgctgcga tttccgccgc gaagacgagc
     1801 acgtgatcga gccgacgcgg ctgccgccct cgacgcgcat cgtggggctc accgtcgatc
     1861 cgggcagcac cagctcgttc ttccagcagg tcgccgagcg cggcgccgga cccgccgaca
     1921 tggcggcgtt catggaagag atcaaccgcg agttcgtgcg ccaccatgcg ccgcagttcc
     1981 gccgcatgct gggcgagctg ctcgcgctgg aggacggcgg cttcatgatc aactgcgccg
     2041 ccggcaagga ccgcaccggc ttcggcgcgg cgatgatcct ggccgcgctg ggtgtggccg
     2101 aggacgatat cgtggccgac tacctgctgt cggatcgcta tttcccgatc gagcgcgaga
     2161 tggatcgcgt gctgcgcaag tacggcggcg aggccggcgg gcgactggat ccgcagctga
     2221 tccgcccgat gatggagacg cgccgcgagt acatcgaagc cgcgctggag gcgatccggc
     2281 gcgactacgg ctcgctggag cgtttcctcg acgaggcgat gggcgtgggc cccgcggagc
     2341 gtcgcgagct gcgcgagcgc ttcaccgcct gatccggctg aaggtcgcgc gcatggcgcg
     2401 ctcctgtagg agcgggccat gcccgcgacc agcccacgac gcgttt
//
LOCUS       DAORUQ010000320         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_64776_length_2441_cov_7.753143, whole genome shotgun sequence.
ACCESSION   DAORUQ010000320 DAORUQ010000000
VERSION     DAORUQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64776_length_2441_cov_7.753143"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..315)
                     /locus_tag="PLW13_16030"
     CDS             complement(103..315)
                     /locus_tag="PLW13_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71939.1"
                     /translation="MRALVIAACLALLTSCASPQRLNLSAEEQAACDAEGGCTVISRM
                     LYDYLMLVHEMALQEQSRQRRGGGSI"
     gene            complement(320..529)
                     /locus_tag="PLW13_16035"
     CDS             complement(320..529)
                     /locus_tag="PLW13_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71940.1"
                     /translation="MKLVPNWRRMWRAFSVQAMTLALAIQGAWAGMPDELKANVPPKL
                     VLWITVGLLVAGIVGRMVDQPKVEE"
     gene            complement(531..716)
                     /locus_tag="PLW13_16040"
     CDS             complement(531..716)
                     /locus_tag="PLW13_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71941.1"
                     /translation="MDEKHAVAVKLTTIWTAVGFSKLGINSWSDVAAICASIYSLLLI
                     GRLLWHWFKVWRNKEGR"
     gene            complement(718..2136)
                     /locus_tag="PLW13_16045"
     CDS             complement(718..2136)
                     /locus_tag="PLW13_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQY71942.1"
                     /translation="MRKVIDLGAQAALLLAAALLPSAGWAATLVQTKVAISAYKGTTK
                     IGTGFATEASCEAFLETQPAMATNYKCRKDATYTLKADPAPTPVDVCPNIAGDQAAVP
                     AGMVKDASGNCVSAPPPPAPPPTLPPGVTVATALPAGYVQCGAENYSAPQTCTSTGPV
                     YYGAGSKWHVRAGPVQCNNIAFGDPVDGMLKTCHQAGSVTPPPPPVEPPPATGDAGPR
                     VTTFTASGPITAVAGQVIAGVRITNSSGPCITINVPNVTVRDSDIGPCEGEANILVGG
                     SAKNTRIEHNSIHDGARGVLASQVDYVDVVKNKFNQFHGPKYKGTAIECDYTNFCTMD
                     GNVVTGRAYASDAVSMYESSNAKIINNTIDIDMAEPSGAAFTMGDSTTGKPGSNNYVA
                     GNVVRQTGGVPAGVFGSNGNTVLEKNCLTAGIQAYNYSGVFVGVTVRNNVIGPGSFVP
                     DTSVIAGWSTNIMGTDCSRVGL"
     gene            2205..>2441
                     /locus_tag="PLW13_16050"
     CDS             2205..>2441
                     /locus_tag="PLW13_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71943.1"
                     /translation="MLVLVLGARARLHAVGVAGGCAALEGQLGAVDTGDELVGSAHAG
                     WEAGGNHGGSELLLDGEVDDVGAGSPRFGEVGEAQ"
BASE COUNT          342 a          792 c          875 g          432 t
ORIGIN      
        1 gcgccttcgc tctcgcgcag tggtgcgaat gcgggcaggc tttcgacggc acgcagcgcg
       61 tcacgtcgtc ggccagcacc acgttcgggt cgcggtccat cgtcagatgc tcccgccgcc
      121 gcgcctctgc cggctctgct cctgcagcgc catctcatgc acgagcatca ggtagtcgta
      181 gagcatccgg ctgatgaccg tgcaaccgcc ctcggcatca cacgcagcct gttcctcggc
      241 cgagaggttc aggcgctgcg gcgaggcgca cgaggtcagc agcgcgagac aggcggcgat
      301 gacgagcgcg cgcatgggcc tactcctcga ccttgggttg atcgaccatg cggccgacga
      361 tgccggccac gagcaggccg acggtgatcc acagcacgag cttgggcggc acgttggcct
      421 tgagttcgtc gggcatgccg gcccacgcgc cttgaatggc gagcgcgagc gtcatcgctt
      481 gaaccgagaa cgcgcgccac atgcggcgcc agttgggtac gagcttcatg tcagcggccc
      541 tccttgttgc gccacacctt gaaccagtgc cacagcagcc ggccgatcag cagcagcgag
      601 tagatcgacg cgcagatcgc tgcgacatcg gaccaagagt tgatgccgag tttcgagaag
      661 cccacggcgg tccagatcgt cgtgagcttg acggcgacgg cgtgcttctc gtccatctca
      721 cagccccacg cgcgagcagt cggtgcccat gatgttggtc gaccaccctg cgatgacgct
      781 cgtgtcgggc acgaacgagc cggggccgat cacgttattg cgcaccgtga cgcccacgaa
      841 gacacccgag tagttgtagg cctggatgcc ggcggtcagg cagttcttct cgaggacggt
      901 gtttccgttg ctgccgaaca ccccggcggg cacgccgccg gtctgccgca ccacgttgcc
      961 ggcgacgtag ttgttgctgc ccggcttgcc cgtcgtcgag tcgcccatcg tgaaagcagc
     1021 gcccgagggc tcggccatgt cgatgtcgat cgtgttgttg atgatcttgg cgttgctcga
     1081 ctcgtacatc gagacggcgt cgctcgcgta ggcgcggccg gtcaccacgt tgccgtccat
     1141 cgtgcagaag ttcgtgtagt cgcactcgat ggcggtgccc ttgtacttcg ggccgtggaa
     1201 ctggttgaac ttgttcttca ccacgtccac gtagtccacc tgcgaggcga gcacgccgcg
     1261 ggcgccatcg tggatgctgt tgtgctcgat gcgcgtgttc ttcgcgctgc cgccgacaag
     1321 gatgttcgct tcgccttcgc acggcccgat gtcggagtcg cgcacggtca cgttcggcac
     1381 gttgatcgtg atgcacggac cgctgctgtt cgtgatgcgc acgccggcga tcacctgccc
     1441 agcgacggcc gtgatcggac ccgaggcggt gaaggtcgtg acgcgcggcc cggcgtcacc
     1501 ggtggccggc ggcggctcga cgggcggcgg aggcggcgtc acgctgcccg cctggtggca
     1561 cgtcttgagc atgccgtcga ccggatcgcc gaacgcgatg ttgttgcact ggaccggccc
     1621 cgcgcgaacg tgccacttgg agccggcccc gtagtaaacc ggcccggtgc tcgtgcacgt
     1681 ctgcggcgcc gagtagttct ccgcgccgca ctgcacatag ccggccggca gcgccgttgc
     1741 cacggtcacg ccgggaggga gggtcggggg tggcgcgggc gggggcggcg cgctcacgca
     1801 gttgccactg gcgtccttca ccatgccggc cggcaccgcc gcctggtcac ccgcgatgtt
     1861 cgggcagacg tcgacgggtg tgggcgcggg gtcggccttg agcgtatacg tcgcgtcctt
     1921 gcggcacttg tagttcgtcg ccatcgcggg ctgcgtctcg aggaacgcct cgcacgaggc
     1981 ctcggtcgcg aagccggtcc cgatcttcgt cgtacccttg taggcgctga tggcgacctt
     2041 cgtctggaca agtgtcgcgg cccatcccgc cgaggggagg agtgcggcgg cgagcagcag
     2101 cgcggcctgc gcgccgaggt cgatcacttt tctcatggct ggttgccctc ctcgggctgg
     2161 ggttcggttg tgaggttgcc gggcgggggc gcgggcggca tcaggtgctc gtgctggtgc
     2221 tcggcgcacg ggcacggctg cacgccgttg gcgtggcagg cggctgcgcg gcgctggaag
     2281 gccagctcgg cgcggtcgac accggtgacg agctggtcgg gtcggcgcac gcggggtggg
     2341 aggcgggagg caatcatggt ggctcagaac tccttctcga cggagaggtg gatgacgtgg
     2401 gcgctggcag cccccgcttt ggggaggtag gagaggcgca g
//
LOCUS       DAORUQ010000321         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_65009_length_2436_cov_5.611928, whole genome shotgun sequence.
ACCESSION   DAORUQ010000321 DAORUQ010000000
VERSION     DAORUQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65009_length_2436_cov_5.611928"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLW13_16055"
     CDS             <1..709
                     /locus_tag="PLW13_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71944.1"
                     /translation="WLLLGDLGVRREKIGNTRIAVGAPTGQGLRRMDIMAFFRWTLPT
                     LQQIFPEFPERILVVGADDPMWRGALSGPGSLFVHSDRPMISENATSTFIHELVHVAM
                     RAKSAPGSDWIVEGLAEYYSLEALHRSGTVSTARFEQAHRTLADWAAREAGPLDAPRS
                     SGATTARAVGVLRKVDAEIRAASGGRQSLDNVVADLAAQPKAVTLEMFRTSTERHAGR
                     VLASLQPAALKGSPTAP"
     gene            complement(727..1497)
                     /locus_tag="PLW13_16060"
     CDS             complement(727..1497)
                     /locus_tag="PLW13_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY71945.1"
                     /translation="MDIAGKVAIITGGASGLGEATVRRLAGMGARVAIFDMNESRGSA
                     VAAELRANVSYHNVNVSSEESVQAAIAAVVARHGALHICCNYAGIANAIKTLGKQGAF
                     PLAEFNKVIQVNLVGTFNVLRLAAEQMAKNEPVNRDGGRGVIINTASVAAYEGQIGQA
                     AYSASKGGVVGMTLPIARDLASYGIRVNTIVPGLINTPLFQALPEPAVKALGQQVLFP
                     QRLGEPDEIAHLAVAIIENDYINGECIRMDGGIRMPPR"
     gene            complement(1552..2256)
                     /locus_tag="PLW13_16065"
     CDS             complement(1552..2256)
                     /locus_tag="PLW13_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY71946.1"
                     /translation="MAEARIFGAFERAVAARYLRARKGERAVSLIAAFSLAGIALGVA
                     TLIVVLSVMGGFRQELLARILGLNGNLGVVAADGPLRDYEAVAARIRALPQVAQAMPT
                     VEGQVLLTTDAGASAGGLVRLPRMVPYRLALELALTGEMIPASRAYELGLLNRIVPAG
                     EALEGALALARSIVANGPLAVATSKRVMRDSAGWSDAEIWPKQNAATAAIFDSEDARE
                     GAAAFAEKRAPVWKGK"
     gene            complement(2249..>2436)
                     /locus_tag="PLW13_16070"
     CDS             complement(2249..>2436)
                     /locus_tag="PLW13_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HQY71947.1"
                     /translation="WLEETGLFGAELRFLMQLPSVVEPGEVAVIVAMGLGLSLLATLW
                     PSWRAARIDPAEALRHG"
BASE COUNT          354 a          892 c          821 g          369 t
ORIGIN      
        1 ctggctgctg ctcggcgacc tcggcgtgcg ccgcgagaag atcggcaaca cccgcatcgc
       61 ggtcggcgcg cccacgggcc agggcctgcg gcgcatggac atcatggcct tcttccgctg
      121 gacgctgccg acgctgcagc agatattccc ggaattcccc gagcgcatcc tggtcgtcgg
      181 tgccgacgac ccgatgtggc gcggcgcgct gtcgggtccg ggctcgctgt tcgtgcactc
      241 cgaccgcccg atgatcagcg agaacgccac cagcacgttc atccacgaac tggtgcacgt
      301 cgcgatgcgc gccaagagcg cgccgggttc cgactggatc gtcgaaggcc tcgccgagta
      361 ctactcgctc gaggcgctgc accgctccgg cacggtgagc acggcgcgct tcgagcaggc
      421 gcatcgcaca ctcgccgact gggccgcgcg cgaagcgggc ccgctcgacg ccccgcgttc
      481 ctcgggcgcg accaccgcgc gcgcggtagg ggtgctgcgc aaggtagatg ccgaaatccg
      541 tgccgccagc ggtggccgcc agagcctcga caacgtggtc gcggatctcg cggcgcagcc
      601 gaaagcggtc acgctggaga tgttccgcac cagcaccgag cgccacgccg ggcgtgtgct
      661 cgcctcactg caaccagctg ccttgaaagg ctcacccact gcaccctgag gcccgctgtg
      721 tgcccttcag cgcggcggca tgcggatgcc gccgtccatg cggatgcact cgccgttgat
      781 gtagtcgttc tcgatgatcg cgaccgcgag gtgcgcgatc tcgtcgggct cgccgaggcg
      841 ttgcgggaac agtacctgct ggcccagcgc cttgaccgcg ggctcgggca gcgcctggaa
      901 cagcggcgtg ttgatcaggc ccggcacgat ggtattgacg cggatgccgt aggaggcgag
      961 atcgcgcgcg atcggcagcg tcatgcccac cacgccgccc ttgctggcgc tgtaggccgc
     1021 ctgcccgatc tggccctcgt aggccgcgac cgaggcggta ttgatgatga cgccacgccc
     1081 gccgtcgcgg ttgaccggct cgttcttcgc catctgctcg gcggccaggc gcagcacgtt
     1141 gaaggtgccg accaggttga cctggatcac cttgttgaac tcggccagcg ggaaggcgcc
     1201 ctgcttgccc agcgtcttga tcgcgttggc gatcccggca tagttgcagc agatgtgcag
     1261 cgcgccgtgc cgggcgacca ccgcggcgat cgcggcctgc accgactcct cgctgctgac
     1321 gttgacgttg tggtagctga cgttcgccct gagctcggcg gccaccgcgc tgccgcggct
     1381 ctcgttcatg tcgaatatcg cgaccctggc gcccatgccc gcgaggcgtc tcacggtggc
     1441 ttcgcccaga cccgaggcgc cgccggtgat gatggctacc tttcctgcaa tgtccattgc
     1501 cgttctcctg ttatcgatga tggttgactg ccgggcgaga cgcggcctgc cctacttgcc
     1561 cttccagacc ggcgcgcgct tctcggcgaa ggccgcggca ccctcgcggg cgtcctcgct
     1621 gtcgaatatc gccgccgtcg cggcattctg cttcggccat atctcggcat cgctccagcc
     1681 ggccgaatcg cgcatgacgc gcttgctggt ggcaacggcc aggggtccgt tggccacgat
     1741 ggatcgcgcc agcgccagcg cgccctcgag cgcctccccc gcgggcacga tgcggttgag
     1801 caggccgagc tcgtaggcac gcgaggccgg gatcatctcg ccggtgagcg cgagctcgag
     1861 cgcgaggcgg tacggcacca tgcgcggcag gcgcaccagc ccgccggcgg aagcccccgc
     1921 atcggtggtc agaagcacct gcccttccac cgtcggcatc gcctgggcga cctgcggcag
     1981 ggcgcggatg cgcgccgcca ccgcctcata gtcgcgcagc ggcccgtcgg cggcgacgac
     2041 gccgagattg ccgttcagcc ccaggatgcg tgccagcaat tcctgccgga acccgcccat
     2101 caccgagagc acgacgatca gcgtcgccac gccgagcgcg atgccggcca gcgagaaggc
     2161 cgcgatcagc gacaccgcgc gctccccctt gcgcgcgcgc aggtaacgcg cggcgaccgc
     2221 gcgttcgaag gcgccgaaga tgcgggcctc agccatggcg cagcgcctcg gccgggtcga
     2281 tgcgcgccgc gcgccagctc ggccacagcg tggcgagcag cgacagcccc aagcccatgg
     2341 cgacgatcac cgcgacctcg cccggttcca cgaccgatgg cagctgcatc aggaagcgca
     2401 gttccgcgcc gaacagcccc gtctcctcca gccagg
//
LOCUS       DAORUQ010000322         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_65522_length_2424_cov_7.696919, whole genome shotgun sequence.
ACCESSION   DAORUQ010000322 DAORUQ010000000
VERSION     DAORUQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65522_length_2424_cov_7.696919"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLW13_16075"
     CDS             <1..757
                     /locus_tag="PLW13_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQY71948.1"
                     /translation="ILGAPNAGKSSLLNALAGVDRAIVTAIPGTTRDVLREAVRVAGV
                     ELILADTAGLRETPDEIEAEGVRRARAELGRADFALLVIDASLAPDQQSRDRDALASE
                     CPPALARVWIETKCDLAPVRGAAPPAGAASAATSAAPVAAEAAPTGAGAATLQVSALT
                     GAGLDALRHELARRAGAGSEGTFSARARHVAALERATTHLARGAEALDAQRAGELLAE
                     ELRLAHQALGEITGQTTADDLLGRIFGEFCIGK"
     gene            complement(808..1917)
                     /locus_tag="PLW13_16080"
     CDS             complement(808..1917)
                     /locus_tag="PLW13_16080"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY71949.1"
                     /translation="MALLGPRQVGKTTLALEMAAMRPVVYLDLESEADRAKLSEPELY
                     LAQHADKLVILDEIQRTPQLFRSLRGIIDAGRRHGRGKGRFLVLGSASIELLRQSSES
                     LAGRIRYLELAPIDAGEAGRNRLGALWLRGGFPDSLLAASDAASLRWRMDFIRTYLER
                     DIPQLGPRIPAETLRRFWTMLAHQQGGLLNAAALARALAVDGKTVAAYLDLLVDLLLV
                     RRLMPWHGNTRKRLVKSPKVYVRDSGLVHALLGIGDREALLAHPVAGASWESLVIESL
                     IGAAPEGTDAHFFRTSAGAEIDLLLKLPGHRQPWAIEIKRGLAPKLERGFHLACESVR
                     PERRWVVYGGVERFPVADGVEAISPGDLCKELSAA"
     gene            complement(2210..>2424)
                     /locus_tag="PLW13_16085"
     CDS             complement(2210..>2424)
                     /locus_tag="PLW13_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71950.1"
                     /translation="IDSNMGHAYVAAEGCDEAVLLGVCSSADEQLMESLLKLHGDDAP
                     TLGMVRAVEPANSAEPRPRARKSRAR"
BASE COUNT          384 a          873 c          830 g          337 t
ORIGIN      
        1 gatcctgggc gcgcccaacg ccggcaagtc gagcctgctc aacgcgctcg ccggcgtcga
       61 tcgcgcgatc gtcaccgcga tccccggcac cacccgcgac gtgctgcgcg aagcggtgcg
      121 cgtggccggc gtcgagctca tcctggccga caccgcgggc ctgcgcgaga cccccgatga
      181 aatcgaagcc gaaggcgtgc gccgtgcccg cgccgagctc gggcgcgccg acttcgccct
      241 gctggtgatc gacgccagcc tcgcgccgga ccagcaatcc cgcgaccgcg acgcgctcgc
      301 cagcgaatgc ccccctgcgt tggcgcgcgt atggatcgag accaagtgcg acctcgcgcc
      361 agttcgcggc gcagcacccc ctgcaggagc ggcttcagcc gcgaccagcg ccgcaccggt
      421 cgcggctgaa gccgctccta caggggcagg tgccgccacg ctgcaagtct ccgccctcac
      481 cggcgccggc ctcgacgccc tgcgccacga actcgcgcgc cgcgccggtg ccggcagcga
      541 aggcaccttc agcgcccgcg cccgccacgt ggccgcactc gaacgtgcca ccacgcacct
      601 cgcacgcggc gccgaggcgc tggatgcgca gcgtgccggt gagctgctgg ccgaggaact
      661 gcgcctcgct caccaggccc tgggcgaaat caccgggcag accacggcgg atgacctgct
      721 gggcaggatc ttcggcgagt tctgcatcgg aaagtagagc cgccgccgat agcaccccgg
      781 atggtcgtgc cggcaaggtg gatcgcctca agccgcggac agctctttgc acaggtctcc
      841 tggcgagatc gcttccacgc catcggcgac cgggaagcgt tcgacgccgc catacacgac
      901 ccagcggcgt tccggccgca ccgactcgca agccaggtgg aagccgcgct cgagcttcgg
      961 cgcgaggcca cgcttgatct cgattgccca aggttggcga tgccctggga gcttgagcag
     1021 gaggtcgatc tcggcgccag ccgaggtccg gaaaaagtgc gcatcggtgc cttccggcgc
     1081 ggccccgatc agcgactcga tcaccaggct ctcccaactg gcgccggcga ccggatgcgc
     1141 cagcagcgcc tcgcgatcgc cgatcccgag cagggcatgc accagcccgc tgtcgcggac
     1201 gtacaccttg ggcgacttga ccaggcgctt gcgggtgttg ccgtgccacg gcatcaggcg
     1261 gcgcacgagc agcaagtcga ccagcaggtc gaggtaggcc gcaaccgtct tgccatccac
     1321 ggccagggcg cgcgcgagcg cggcagcgtt gagcaaaccg ccctgctggt gcgcgagcat
     1381 cgtccagaac cggcgcagcg tctcggccgg gatgcgcggc ccgagttgcg gaatgtcgcg
     1441 ttcgaggtag gtacggatga agtccatgcg ccagcgcagg ctcgccgcat ccgatgcggc
     1501 cagcaggctg tccgggaagc cgccgcgcag ccatagcgcc ccgagacgat tgcggcctgc
     1561 ctctcccgca tcgatcgggg cgagttccag gtagcggatg cggccggcca gcgactcgct
     1621 cgattgccgg agcagttcga tcgaggccga gcccagtacc aggaagcgcc ccttgccgcg
     1681 gccatgccgc cggccggcgt cgatgattcc gcgcaggctg cggaagagct gcggcgtgcg
     1741 ctggatctcg tcgaggatca cgagcttgtc cgcatgctgc gccaggtaaa gctcgggttc
     1801 ggagagtttc gcccgatcgg cctcggattc gaggtcgagg tagaccaccg gccgcattgc
     1861 cgccatctcg agcgccaacg tggtcttgcc cacctggcgc ggcccgagca gcgccacggc
     1921 cgggacttca tccagggagg aggcgagagc cggttggagt cgacggggaa tcatgctagc
     1981 aattatggag tgacattccg caattgcaag gcactcgggc gacttggctg agccatgcgc
     2041 gggcgggcgg tgcgctacgc ctcatcgcat ccaccgcggc cggcagcacg cgtagccggc
     2101 gggcgagtac ggcgtgcacc aggggggaag ggtcttcggg tcatgaatga cattcattca
     2161 cgacgcggat tggcaggcct gtgcccaggc ccctgcatcg ccccgcagac tagcgcgcgc
     2221 gcgacttgcg cgcgcgcggc cgcggctccg ccgaattcgc cggctccacc gcgcgcacca
     2281 tgcccagcgt cggcgcgtcg tcgccgtgga gcttgagcag cgattccatc agctgctcgt
     2341 cggcgctcga gcacacgccc agcaacaccg cctcgtcgca gccttccgcg gcgacgtagg
     2401 cgtggcccat gttggaatcg atgt
//
LOCUS       DAORUQ010000323         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_66820_length_2395_cov_7.189316, whole genome shotgun sequence.
ACCESSION   DAORUQ010000323 DAORUQ010000000
VERSION     DAORUQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66820_length_2395_cov_7.189316"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLW13_16090"
     CDS             <1..701
                     /locus_tag="PLW13_16090"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQY71951.1"
                     /translation="SDISVTSFSIFHIITCLGNGGLIAVNDPKLWDRALMLRAWGRSS
                     EKFMHGTRKNDSDGRFLENLGDIEYDGLFIFEEIAYGFIPNEAGAAFGHEQLNKLDLF
                     TKLRQERFDLHTQYMDKRADIFIKPRVLDEVFTTWICYPIQLRPELGWSRRELQMHLE
                     TNGIFTRVIMSGHTALQPMMEKVKFRVDPEGCPNAERVMRHGLMLPCHPTMTLEDCQY
                     VYQTIDDFIAMQRA"
     gene            781..978
                     /locus_tag="PLW13_16095"
     CDS             781..978
                     /locus_tag="PLW13_16095"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQY71952.1"
                     /translation="MKVDVIPDVCQGHARCQDRCPEVFSTDPVEGKCVILMADVPPEL
                     EQKARLAVKNCPEGALIIVSD"
     gene            1010..2239
                     /locus_tag="PLW13_16100"
     CDS             1010..2239
                     /locus_tag="PLW13_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQY71953.1"
                     /translation="MTAHRYFPGPPDHVPAGLIHEFDIYQFSPGVNDPLEQWGNLLAS
                     DAPRIFYTHHNGGHWVFLDYEDVREAFRNDARFSTFQTPIPPIEPYPVMQPQGVDPPE
                     HKKFRSLLAPLFTPSAVGKMQAELRRRTTALLEAFVDKGRCEFGRDFASVLPTGMFLY
                     LMGMPEDRLTEFVKLAEVFMRSNDEAERAQNIQQIYAVIAEYLAWRSTRLGDDLGSVL
                     ISARDENGQPFDQQDVLNCGFLLFVAGLDTVTSTMTFIWRHLARNAEARRELVGMIDD
                     REKLTVAVDELLRIHSVPNIYRRVKKDMVYRGITMKENDRVVLPVSVANRDGKVFEHA
                     DEIDLHREVNNHLTFGAGPHRCVGSHLAKTEVTIALQEWLRRIPEFDVAADAQIRGHL
                     GPTLGFSQLPLVWKPKA"
BASE COUNT          471 a          810 c          728 g          386 t
ORIGIN      
        1 gcagcgacat cagcgtgacc agcttctcga tcttccacat catcacctgc ctgggaaacg
       61 gcggcctgat cgccgtcaac gatccgaaac tgtgggatcg cgcgctgatg ctgcgcgcct
      121 ggggccgctc ctccgagaag ttcatgcacg gcacgcgcaa gaacgacagc gatggccgct
      181 tcctggaaaa cctcggcgac atcgaatacg acggcctgtt catcttcgag gaaatcgcct
      241 acggcttcat cccgaacgag gccggcgcag cgttcggtca cgagcagctg aacaagctgg
      301 acctgttcac gaaattgcgc caggagcgct tcgacctgca cacgcaatac atggacaagc
      361 gcgccgatat cttcatcaag ccgcgcgtgc tggacgaggt gttcaccacc tggatctgct
      421 acccgatcca gttgcgcccg gaactcggct ggagccgtcg cgagctgcag atgcacctgg
      481 aaaccaacgg catcttcacc cgcgtgatca tgtccggaca caccgcgctg cagccgatga
      541 tggagaaggt gaagttccgc gtggacccgg aaggctgccc gaacgccgaa cgcgtgatgc
      601 gccacggcct gatgctgccc tgccacccca cgatgacgct cgaggattgc cagtacgtgt
      661 accagaccat cgacgatttc atcgccatgc agcgcgcgta ggtcaggctt cagccggaca
      721 cgtgcaggat gtccgcatga atgctgacct acggccggac ttcgattgag gagaaaccac
      781 atgaaagtcg acgtgatacc cgatgtatgc cagggccacg cgcgctgcca ggaccgctgt
      841 ccggaagtct tctccaccga cccggtcgaa ggcaagtgcg tcatcctgat ggctgacgtg
      901 ccacccgagc tcgagcagaa ggcacgcctg gccgtgaaga actgccccga gggcgcattg
      961 atcatcgtga gcgactgaaa ccgataccgt cccccgggag caccggacca tgaccgcaca
     1021 ccgctatttc cccggcccac ccgaccacgt gccggcggga ctgatacacg agttcgacat
     1081 ctaccagttc tcgccgggcg tgaacgatcc gctcgagcaa tggggcaatc tgctggcgag
     1141 cgacgcgccg cgcatcttct acacgcacca caacggcggt cactgggtgt tcctcgacta
     1201 cgaggacgtg cgcgaggcat ttcgcaacga cgcccggttc tcgaccttcc agacgccgat
     1261 cccgccgatc gagccctacc cggtcatgca gccgcagggc gtggatccgc cagagcacaa
     1321 gaaattccgc tcgctgctcg caccgctgtt cacgccgtcg gccgtcggga agatgcaggc
     1381 ggaactgcgc cggcgcacca ccgcgctgct cgaggcgttc gtcgacaagg gccgttgcga
     1441 gttcggccgc gacttcgcct cggtgctgcc gacgggcatg ttcctttacc tgatgggcat
     1501 gccggaggat cgcctgacgg aattcgtcaa gctggccgag gtgttcatgc gcagcaacga
     1561 cgaggccgag cgcgcgcaaa acatccagca gatctacgcc gtgatcgccg aatacctcgc
     1621 gtggcgctcc acgcggctcg gcgacgatct cggcagcgtg ctgatcagcg cccgggacga
     1681 gaacgggcaa ccgttcgacc agcaggacgt gctcaactgc ggcttcctgc tgttcgtcgc
     1741 cggactggac accgtgacca gcacaatgac cttcatctgg cgtcacctcg cgcgcaacgc
     1801 agaggcacgg cgcgagctcg tgggcatgat cgacgatcgc gagaagctca cggtggccgt
     1861 cgacgaactg ctgcgcatac actcggtacc gaacatctac cgtcgcgtga agaaggacat
     1921 ggtctaccgc ggcatcacga tgaaggagaa cgaccgcgtg gtgcttcccg tgagcgtcgc
     1981 caaccgcgac gggaaagtgt tcgagcacgc ggacgagatc gacctgcatc gcgaggtcaa
     2041 caaccacctg acctttggcg ccggcccgca ccgttgcgtc ggttcgcacc tggccaagac
     2101 cgaggtcacg atcgcgctgc aggaatggct gcgacgcatc cccgagttcg acgtcgccgc
     2161 cgatgcacag atccgcggcc acctcggccc gacgctcggt ttcagccagc tgccgctggt
     2221 ctggaaacca aaggcatgag cctcccggga cacgccccgc tcgagggcct gcccgcggcc
     2281 gaccagatct gcctggtcgt gcgcgacctg gaagccgcgg tggcgatgta cgaaccgctg
     2341 ttcggcccgt tcacggtgct cgacggttca agagcccccc tgcgcatcgg cgacc
//
LOCUS       DAORUQ010000324         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_67095_length_2389_cov_6.082262, whole genome shotgun sequence.
ACCESSION   DAORUQ010000324 DAORUQ010000000
VERSION     DAORUQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67095_length_2389_cov_6.082262"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLW13_16105"
     CDS             complement(<1..233)
                     /locus_tag="PLW13_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQY71954.1"
                     /translation="MSNFNVETVTGVRHWNDSLFSFTTTRDSGFRYRNGHFTMVGLEV
                     ENRPLVRAYSIVSANHEQELEFFSIKVQDGPLTS"
     gene            353..1234
                     /locus_tag="PLW13_16110"
     CDS             353..1234
                     /locus_tag="PLW13_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY71955.1"
                     /translation="MKYTLRQLEVFLATARAQTLSQAAQELAMSQSAASDALAGLEQQ
                     FEVRLFDRIGKRLQLNETGRSLLPAAEELLVRARELERVLGRKDTAGALAVGATLSIG
                     NYLAVPIMLRYMQENPGARVTLDVANTAAIAQKIARFELDVGLIEGEIRHPELELAPW
                     RDDELVVFCAPQHPLAKRRRLGERELLAATWILREPGSGTRQAFDRAMHGLLAQLNVL
                     LELQHTEAIKRAVESGIGIGCLSRATLQEAFQRGSLVPLAVPGRDFTRQFYIVLHRRK
                     YRSAGIRRWVELCRAIT"
     gene            complement(1227..>2389)
                     /gene="glcF"
                     /locus_tag="PLW13_16115"
     CDS             complement(1227..>2389)
                     /gene="glcF"
                     /locus_tag="PLW13_16115"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013973100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HQY71956.1"
                     /translation="FCTATCPTYQLLGDELDGPRGRIYLIKEMLAGAEATTVTQTHLD
                     RCLSCRACETTCPSGVEYGRLLDIGRKVAHKRIGRSPGQRLQRWALRRAMADRRVFGA
                     LLGTGRALRALLPRALRAMIAPRRSPGEWPAPVHARRMLVLDGCVQPAIAPRINAAAA
                     RVLDRLGISLLRVARAGCCGALSQHLDAQDEALAFARRNIDAWWPLLEQGAEAIVMTA
                     SGCGVMVKDYAQLLRDDAAYAAKAARVSAATKDLAEILAVEGRSLAPQAGGPRVAFQC
                     PCTLQHGQKLGGVVEGLLLRAGCELVPVADAHLCCGSAGTYSLLQPELSQRLRDDKLR
                     ALQAGRPGFIATANIGCLNHLQARSEVPVLHWIELLDPGVDLDGGPASAGPM"
BASE COUNT          386 a          833 c          808 g          362 t
ORIGIN      
        1 gaggtcagcg ggccgtcctg caccttgatg ctgaagaact ccagctcttg ctcgtggttc
       61 gcgctgacga tgctgtaggc acgcaccagc gggcggttct ccacttcgag gccgaccatc
      121 gtgaagtgcc cgttgcgata gcgaaagccg ctgtcgcgcg tcgtggtgaa gctgaacagg
      181 ctgtcgttcc agtgacgcac gcccgtgacc gtctcgacgt tgaaattgct catggtgctc
      241 tcccgatggc ggcccgcacc gtgcgtgcct gcccgaccgg cgccacttta aggagcggac
      301 catatatcgg taaaatggtt tttaccaata tctgtattcg ggtttatcga ttatgaaata
      361 caccctgaga caactcgagg tattcctggc caccgcgcgt gcgcagacgc tcagccaggc
      421 ggcgcaggag ctggcgatgt cgcagtcggc cgcgagcgat gcgctcgccg ggctcgaaca
      481 gcaattcgag gtgcggctgt tcgatcgcat cggcaagcgc ctgcagctga acgagaccgg
      541 tcgcagcctg ctgcccgccg cggaggagct gctggtgcgc gcgcgcgaac tggaacgcgt
      601 actcgggcgc aaggataccg ccggcgcgct ggcggtgggc gccacgctca gcatcggcaa
      661 ctacctcgcg gtgcccatca tgctgcgcta catgcaggag aacccgggcg cacgcgtgac
      721 gctggatgtc gcgaacaccg cggcgatcgc gcagaagatc gcgcgcttcg agctcgacgt
      781 cgggctgatc gagggcgaga tccgccatcc ggagctggag ctggccccgt ggcgtgacga
      841 cgaactggtg gtcttctgcg cgccgcagca tccgctggcg aaacgccggc gactcggcga
      901 gcgcgagctg ctggcggcga cttggatcct gcgcgagccg ggctccggca cgcgccaggc
      961 cttcgaccgc gccatgcacg gcttgctggc gcagctgaac gtgctgctgg aactgcagca
     1021 caccgaggcc atcaagcgcg ccgtcgagag cggcatcggc atcggctgcc tgtcgcgagc
     1081 gacgctgcag gaggccttcc agcgcggcag cctggtaccg cttgccgtgc cgggccgcga
     1141 cttcacgcgc cagttctaca tcgtgctgca ccggcgcaag taccgcagcg caggcatacg
     1201 gcgatgggtc gagctgtgcc gcgcaatcac ataggtccgg ctgaagccgg acccccatcg
     1261 agatccacgc cgggatcgag cagctcgatc cagtgcagca cgggaacctc ggaccttgcc
     1321 tgcaggtgat tcaggcagcc gatattcgcg gtggcgatga atccgggtcg cccggcctgc
     1381 agtgcccgga gcttgtcgtc acgcaggcgc tgcgagagtt cgggctgcag cagcgagtag
     1441 gtgccggcgg agccgcagca cagatgcgca tccgccaccg gcacgagctc gcagccggcg
     1501 cgaagcagca ggccttccac caccccgccc agcttctggc cgtgctgcag cgtgcacggg
     1561 cactggaatg ccacgcgcgg gccacccgcc tgcggtgcga gtgaccgtcc ttccaccgcg
     1621 agtatctccg cgagatcctt cgtcgccgcc gacacgcgcg cggccttcgc cgcgtaggcc
     1681 gcatcgtcac gcagcagctg agcgtagtcc ttcaccatca cgccgcagcc gctcgcggtc
     1741 atcacgatgg cctcggcgcc ctgctccagc aacggccacc aggcgtcgat gttgcgccgc
     1801 gcgaaggcga gcgcctcgtc ctgcgcgtcc aggtgctgcg acagcgcgcc gcagcagccc
     1861 gcccgcgcca cgcgcagcag gctgatgccc aggcgatcca gcacgcgcgc cgcggcggca
     1921 ttgatgcgtg gcgcgatcgc gggctgcacg cagccgtcca gcaccagcat gcgtcgcgcg
     1981 tgcaccgggg cgggccactc acccggactg cgacgcggcg caatcatcgc gcgcagcgcg
     2041 cgtggcaaca gcgcgcgcag cgcgcgcccg gtacccagca gcgcgccgaa cacgcgccga
     2101 tcagccatcg cgcggcgcag cgcccagcgc tgcaggcgct gccccggcga tctgccgata
     2161 cgtttatgcg cgaccttgcg tccgatatcg agcaggcgac cgtactccac ccccgagggg
     2221 cacgtcgttt cgcaggcgcg gcagctcagg cagcggtcga gatgtgtctg cgtcaccgtc
     2281 gtggcctcgg cgcccgcgag catttccttg atcaggtaga tgcgcccgcg cgggccatcg
     2341 agctcgtcgc cgagcaactg gtaggtcgga caggtcgcgg tgcagaagc
//
LOCUS       DAORUQ010000325         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_67519_length_2380_cov_5.498495, whole genome shotgun sequence.
ACCESSION   DAORUQ010000325 DAORUQ010000000
VERSION     DAORUQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67519_length_2380_cov_5.498495"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLW13_16120"
     CDS             <1..554
                     /locus_tag="PLW13_16120"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQY71957.1"
                     /translation="QHPVPAQIDERACFDAIALAKDVDGVTCLGFGRMAMGEHAWGSA
                     TPAGIMRLLEAYDIPLTGRHAVVVGRSPILGKPMALMLLNANCTVTICHSRTQGLAQL
                     LRQADIVVGAVGKPEFIRADWIRDGAVVVDAGYHPGGIGDIELAPLTERVAAYTPVPG
                     GVGPMTINTLILQTVQAAERALA"
     gene            complement(574..1275)
                     /locus_tag="PLW13_16125"
     CDS             complement(574..1275)
                     /locus_tag="PLW13_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQY71958.1"
                     /translation="MQGFWRAGAVVLGAMLALVPAVAGEERGLDYNLVRLEADAAAEV
                     PNDLMRVTLAVEHEARDAAALPPLVNADMRWALDLAKQRPTVKAQSGGYTTQPEYASG
                     RIVGWRAIQVLELESEDFAEVTALATELQQKLQVRGMEFAPTRATRLRVENELTAAAL
                     EAFAAKAKLIAQTMGAARYEVVELNVGGAPLYKSQRGAEIAMMRSADAAAPPIAVDGG
                     TATLTVTVNGQIQLR"
     gene            1422..1676
                     /locus_tag="PLW13_16130"
     CDS             1422..1676
                     /locus_tag="PLW13_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY71959.1"
                     /translation="MPSYDYRCLATGTVHEVKHSLSQKATTWAELCALGNLAPGDIPP
                     DSPVERLISAAGVVSSGALRNPEPPCGGGGCGRGACSFAG"
     gene            1673..>2380
                     /locus_tag="PLW13_16135"
     CDS             1673..>2380
                     /locus_tag="PLW13_16135"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HQY71960.1"
                     /translation="MNIARGAQALADFVRQHPRLVVLTGAGCSTPSGIPDYRDARGNW
                     KPGPPVLGAQFVRELPARQRYWARSLNGWPRFRAAEPNAAHRALASLEQRGHVRHLIT
                     QNVDGLHQRAGSRAVTELHGSLARVRCLGCDAVCARAEVQRVLERDNPGFRNLDAAPA
                     PDGDAQLETQRLGGFRVPDCPRCGGTLKPDVVFFGESVPAPTVAACLDAIAQADALLC
                     VGTSLMVYSGFRFCRHAA"
BASE COUNT          329 a          889 c          819 g          343 t
ORIGIN      
        1 tgcagcaccc ggtgccggcg cagatcgacg agcgtgcctg cttcgacgcg atcgcgctgg
       61 cgaaggacgt cgacggcgtg acctgcctcg gcttcggccg catggccatg ggcgagcatg
      121 cgtgggggtc ggccaccccg gccggcatca tgcgcctgct cgaggcctac gatatcccgc
      181 tcacggggcg ccatgcggtg gtggtgggac gcagcccgat cctcggcaag ccgatggcgc
      241 tgatgctgct gaacgccaac tgcacggtca ccatctgcca ctcgcgcacg cagggcctgg
      301 cgcaactgct caggcaggcc gacatcgtcg tgggtgccgt gggcaagccg gaattcatcc
      361 gcgcggactg gataagggac ggcgcggtgg tggtcgatgc cggctaccat ccggggggca
      421 tcggcgacat cgaactcgcc ccgctcacgg agcgcgtcgc ggcctacacc ccggtgcccg
      481 gcggcgtcgg gccgatgacg atcaacacgc tgatcctgca gaccgtgcag gcggccgagc
      541 gcgcgctcgc ctgagcgcgc aacgccccgg cgttcagcgc agctggatct gcccgttcac
      601 cgtgacggtg agcgttgccg tgccgccatc gacggcgatg ggcggcgcgg ccgcatcggc
      661 gctacgcatc atcgctatct cggcaccgcg ctgcgacttg tagagcggcg cgccgccgac
      721 gttcagctcg accacctcgt agcgcgcggc acccatggtc tgcgcgatca gtttcgcctt
      781 cgcggcgaag gcctcgagcg ccgcggcggt gagctcgttc tcgacgcgca ggcgggtcgc
      841 gcgggtgggc gcgaattcca tgccgcgcac ctggagcttc tgctgcagct cggtggcgag
      901 cgcggtgacc tcggcgaagt cctcgctctc gagctcgagc acctggatcg cgcgccagcc
      961 gacgatgcgc cccgaggcgt attcgggctg cgtggtgtag ccgccgctct gcgccttcac
     1021 cgtcgggcgc tgcttcgcca gatccagcgc ccagcgcata tcggcgttca ccagcggcgg
     1081 cagcgcggca gcgtcgcgcg cctcgtgctc gacggccagc gtcacgcgca taaggtcgtt
     1141 cggcacctcg gcggccgcgt cggcctccag ccgcaccagg ttgtagtcga gcccccgctc
     1201 ctccccggcc acggccggca ccagcgccag catcgcgccc agcaccaccg ctcccgcgcg
     1261 ccaaaaacct tgcattgccc cgctctccct ggtctgactg gctgccgcca tcgtaaccga
     1321 cgcacgcgac attgccacgc gccgaggctg cccaccgcgc ggcgcgggcg taaaatggcg
     1381 gccgccatgc aattgtcacg tcctgctcac aggagacccc gatgcccagc tacgactatc
     1441 gctgcctcgc caccggaacc gtccacgaag tgaagcattc gctcagccag aaggccacga
     1501 cctgggccga gctgtgcgcg ctgggcaatc tcgcacccgg cgacatcccg ccggattccc
     1561 cggtcgagcg cctgatcagc gccgccggcg tggtcagcag cggcgcgctg cgcaaccccg
     1621 agccgccctg tggcggcggt ggctgcgggc gcggtgcgtg cagcttcgcc gggtgaacat
     1681 cgcccgtggt gcacaggcgc tggccgactt cgtccgccag cacccgcggc tggtggtcct
     1741 caccggcgcg ggctgcagta cgccttccgg catacccgac taccgcgacg cgcgcggcaa
     1801 ctggaagccc ggcccgccgg tgctgggcgc gcaattcgtg cgcgagctcc cggcgcgcca
     1861 gcgctactgg gcgcgcagcc tgaacggatg gccccgcttc cgggccgcgg aacccaatgc
     1921 cgcgcatcgc gcactggcca gcctcgagca gcgcgggcac gtgcgccacc tcattaccca
     1981 gaacgtcgac ggtctgcacc agcgcgccgg cagccgggcc gtgaccgagc tgcacggttc
     2041 gctggcgcgc gtgcgctgcc tcggctgcga cgccgtatgt gcgcgtgccg aggtgcagcg
     2101 cgtgctggaa cgggacaacc cgggattcag gaacctggac gcagcacccg cgccggacgg
     2161 cgacgcgcaa ctggagacgc agcgcctggg cgggttccgc gtaccggact gcccgcgctg
     2221 cggcggcacg ctgaagccgg acgtggtgtt cttcggcgag tcggtgccgg caccaacggt
     2281 cgccgcctgc ctcgatgcga tcgcacaggc cgatgccctg ctctgcgtcg gcacctcgct
     2341 gatggtgtac tcgggattcc gcttctgccg ccacgccgcc
//
LOCUS       DAORUQ010000326         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_67609_length_2378_cov_8.922084, whole genome shotgun sequence.
ACCESSION   DAORUQ010000326 DAORUQ010000000
VERSION     DAORUQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67609_length_2378_cov_8.922084"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2192)
                     /locus_tag="PLW13_16140"
     CDS             complement(132..2192)
                     /locus_tag="PLW13_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY71961.1"
                     /translation="MPDFQLALAHARSGQRAHAEHVCRGLLEANPRDVDALELLGVLA
                     MERGSSAESLALLQQAMLVDPSRPGVHFAHGNAALAMARHKEALASYERVLQLAPGSV
                     DAMRRRADTLLLLGRADEAARGYQSALEANPGQPDVALQLGGALLQAGRAAHAREAFE
                     WLLARWPDDVRAWVNHGNALFALGEFDQALASYERAIGLAPRLAEAHDNRGMALLALG
                     RAEEAIASHGAAIEFAPGFAPAWYRRAMATRATGRHAEALPDAEHALALAPGDALAWN
                     ARGVVRNDLGQYAAALDDFRQAIRIAPGMVEAWNNAGNALHDLGRFDEAIAHFERALS
                     MRPDYPEALSNRGLSLQELSRYGEARAAFDAAIAARPDFAEALKRRAGLRLLQGDFDG
                     GWRDYGASLATRPAVAGGRHGLPAWNGESLRGKSILLTEPNGFGDVFQYWRFVPRLLE
                     MGAQVTFLGDPRLFRLLGSGGLAVTFIGSIDEAGQGFDFHCELWSLPRVMHVDPFGLP
                     APAPWLFAEPQRSGQWRHLLRPGAFNIGIAWQGKPGRKIDSGRSIPLREFVALAGLPD
                     VQLLSLQRGEGLEQLHALAGEMSIVDPGPLFDTGEDAFLDTAALVQELDLVVCSDSAI
                     AHLAGALGRPAWIALKHVPEWRWLLDRGDSPWYPGARLFRQQRRGDWAGVFAQMREAL
                     AARG"
BASE COUNT          386 a          879 c          784 g          329 t
ORIGIN      
        1 ccggttcgac ctttcgacgc ccggccatcg cgccgggcgt cttcttgcgc ccgcggcacc
       61 tggcccggct tgcgaccgtg gtttgagcgc aacccgcgct tcgacgcggc cgctggccgg
      121 cgccggaaag atcagccgcg cgcggcaagg gcctcgcgca tctgtgcgaa gacgcccgcc
      181 caatcgccgc gtcgctgttg ccggaacagg cgggcacccg gataccaggg cgagtcgccg
      241 cgatcgagca gccagcgcca ctccggcacg tgcttcaacg cgatccatgc cggccggccc
      301 agcgctccgg ccaggtgtgc gatcgcgctg tccgagcaga cgaccaggtc cagttcctgc
      361 accaatgccg ccgtatcgag gaatgcgtct tcgccggtat cgaaaagcgg gccgggatcg
      421 acgatcgaca tctcgcccgc aagtgcatgc aactgctcca ggccttctcc acgctgaagg
      481 ctgagcagct gcacgtccgg cagtccggcc agggcgacga attcgcgcag ggggatcgag
      541 cggccactgt cgatcttgcg gccaggcttg ccttgccagg cgatgccgat gttgaaggcg
      601 cccgggcgca gcaggtgccg ccactggccg ctgcgctgcg gttcagcgaa cagccacggc
      661 gccggcgccg gcaatccgaa cgggtccaca tgcatcaccc gcggcaggct ccacaactcg
      721 caatggaagt cgaatccctg cccggcttca tcgatggagc cgatgaaggt gactgcaagc
      781 ccgcctgaac caagcaggcg aaacagtcgg ggatcgccga ggaacgtgac ctgcgccccc
      841 atctccagca gccggggcac gaagcgccag tactggaaca cgtcaccgaa cccgttcggc
      901 tccgtcagca ggatcgactt gccgcgcagc gactcgccgt tccaggccgg gagcccgtgc
      961 cggccgccgg ccacggccgg ccgtgttgcg aggctcgctc catagtcccg ccagccgcca
     1021 tcgaagtcgc cctgcaagag gcgcagcccg gcgcgacgct tgagggcttc ggcaaagtcg
     1081 ggacgcgcgg cgatcgcagc gtcgaacgcg gcccgcgcct cgccgtaccg cgaaagctcc
     1141 tgcagcgaca ggccgcggtt gctgagcgcc tcgggataat ccggtcgcat cgacagcgca
     1201 cgctcgaagt gggcgatcgc ctcgtcgaag cgcccgaggt cgtgcagggc gttgccggcg
     1261 ttgttccacg cctcgaccat gcccggggcg atccggatcg cctggcgaaa gtcgtcgagc
     1321 gcggcggcgt actggcccag gtcattgcgc acgacgccac gtgcattcca ggccagggca
     1381 tccccgggcg caagcgcgag cgcgtgctcc gcatccggca gggcttccgc atggcgaccg
     1441 gtcgcccggg tcgccatcgc ccgtcggtac caggctggcg cgaaaccggg agcgaactca
     1501 atcgccgcac catggctggc gatcgcctcc tccgcgcggc cgagcgccag caaggccatg
     1561 ccacggttgt cgtgcgcttc cgccaatcgc ggggccaggc cgatcgcacg ttcgtagctc
     1621 gcaagcgcct ggtcgaactc gccgagcgcg aacagggcat tgccatggtt cacccatgca
     1681 cgcacgtcgt caggccaacg cgccagcaac cactcgaagg cttcccgcgc atgcgcggca
     1741 cggcccgcct gcagcagggc accacccagt tgcagggcca cgtccggctg ccccgggttg
     1801 gcctcgagcg ccgactggta gccgcgcgcc gcctcatcgg cgcgaccgag cagcaacagc
     1861 gtgtctgcgc gcctgcgcat ggcatccacc gatccgggtg ccaattgcag cacgcgctcg
     1921 taactcgcca gcgcctcctt gtgcctcgcc atcgccagcg ccgcattgcc atgcgcgaaa
     1981 tgcacgcccg ggcgcgaagg atcgaccagc atggcctgct gcaacagggc cagcgactcc
     2041 gctgaggaac cgcgctccat cgccagtacg ccaagcagtt ccagcgcatc cacgtcgcgt
     2101 ggattcgcct ccagcaggcc gcggcacaca tgctcggcat gcgcgcgttg tccgctgcgc
     2161 gcatgcgcaa gcgcaagctg gaaatcgggc aatcgggaat tcatggcagt cctgggtctg
     2221 gcgagcgcgc atttaagcag aggtggtggt gcccgtcatt gccggcccgg cacccgccgg
     2281 ccgcctgaag cgcaccgtcg ggtcgccgcc gcggtcagcc gccggcaagc gcgaacccga
     2341 tcccggcgca caccgccgcc gcctgcgcgt cgttgcat
//
LOCUS       DAORUQ010000327         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_69004_length_2349_cov_4.339582, whole genome shotgun sequence.
ACCESSION   DAORUQ010000327 DAORUQ010000000
VERSION     DAORUQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69004_length_2349_cov_4.339582"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PLW13_16145"
     CDS             complement(<1..173)
                     /locus_tag="PLW13_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005734079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQY71962.1"
                     /translation="MTIIDLPHGAHRFCVAPMLDLTDRHCRYFHRQLSRRARLYTEMV
                     TTGALLHGPRERLL"
     gene            complement(170..532)
                     /locus_tag="PLW13_16150"
     CDS             complement(170..532)
                     /locus_tag="PLW13_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY71963.1"
                     /translation="MSSGERRPDDAVMVRPRVQIGASIAIGPGKIDLLRAVERERSIS
                     AGARALGLTYKRAWLLIDSLNQGFGQPVVAATAGGRGGGGATLTPLGAALVARYAALE
                     ARLDAACGAELQALRELS"
     gene            complement(529..1623)
                     /locus_tag="PLW13_16155"
     CDS             complement(529..1623)
                     /locus_tag="PLW13_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY71964.1"
                     /translation="MSLALALHHDGPPLDFVAEAPAGRITALVGPSGSGKTSILRAIA
                     GLLRLKQARIAFNGEVWDDERTHRETRARPIGLVPQGYALFPHLTVQANVETALLHVA
                     PWQRRQRAGECIALAHVAGLERRRPHELSGGQKQRVALARAIARDPQVLLLDEPFAAV
                     DRSTRKRLHVELRRMHARLGNTIVLVTHDLDEAAQLASQLLLVRHGRLLQAGPIGEVL
                     TRPSCEEAARLLDIPNVFEARASAAAAGATLRLQWGPHVLSAVARQPVADGSPLRWAV
                     LPTNVLLVRADKPQHHLENPVGATVDEVVELGAEALVWLRPHGVAHARLQMRLPVRAV
                     HRHAVRAGAQVTVSLRAGDLVPLALHGEHR"
     gene            complement(1620..>2349)
                     /gene="modB"
                     /locus_tag="PLW13_16160"
     CDS             complement(1620..>2349)
                     /gene="modB"
                     /locus_tag="PLW13_16160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HQY71965.1"
                     /translation="ARDPAAPRLRARRRGRAMMDWPSILLSVELALATLAVLLPTALF
                     AARWLAHTGVRGKAWIEGLLALPLVLPPTVIGYYLLVAMGGNSPLGRWFESLSGHTLA
                     FSFPGLVAASVVFNIPFAVQPMQRAFEAIPQEIHEAARCCGLGVRQALWRIELPLAWP
                     GILSAAVMTFAHTLGEFGVVLMVGGNIAGETRTVAISIYDRAQAFDHAAAGAMSLLLL
                     AISLVTIAVSYGLAERLMRHRRAT"
BASE COUNT          357 a          879 c          822 g          291 t
ORIGIN      
        1 agcagccgtt cgcgcggccc gtgcagcagc gcgccggtgg tcaccatctc ggtatacaac
       61 cgggcccggc ggctgagctg tcgatggaag tagcggcagt gacgatcggt cagatcgagc
      121 atcggcgcaa cgcagaagcg gtgcgcgccg tgcggcagat cgatgatcgt catgacagtt
      181 cgcgcaacgc ctgcagctcg gcgccgcagg ccgcatcgag ccgggcctcg agcgccgcat
      241 agcgcgccac cagcgccgcg ccgagcggcg tgagcgtagc gccgccgccg ccccggccac
      301 cggcggtggc ggcgaccacc ggttgtccga atccctggtt cagcgaatcg atcagcagcc
      361 aggcccgctt gtaggtgagc ccgagcgcgc gtgcgccggc cgagatcgag cgctcgcgct
      421 cgacggcgcg cagcaggtcg atcttgccgg ggccgatggc gatgctcgcg ccgatctgca
      481 cccgcgggcg caccatcacc gcgtcgtccg gccggcgctc gccgctgctc atcggtgttc
      541 tccgtgaagc gccagcggca ccaggtcgcc ggcgcgcagg ctcacggtca cctgcgcacc
      601 ggcgcgcacc gcgtgccggt gcaccgcccg gaccggcagg cgcatctgca gccgcgcatg
      661 cgccacgccg tgcgggcgca accagaccag cgcctcggcg ccgagctcga ccacctcgtc
      721 gacggtggcg ccgaccgggt tctcgaggtg gtgctgcggc ttgtcggcgc gcaccagcag
      781 cacgttggtc ggcaacaccg cccagcgcag cgggctgccg tcggcgaccg gttggcgcgc
      841 caccgcgctc agcacatgcg gcccccactg caggcgcagc gtcgcaccgg ccgcggcggc
      901 cgatgcgcgc gcctcgaaca cgttgggaat gtcgagcagc cgcgccgctt cctcgcacga
      961 cggccgcgtc agcacttcgc cgatcggacc cgcctgcagc aggcgtccgt ggcgcaccag
     1021 cagaagctgc gaggccagct gcgccgcctc gtcgagatcg tgggtgacca gcacgatggt
     1081 gttgccgagc cgggcatgca tgcgacgcag ctcgacgtgc aggcgctttc gcgtgctgcg
     1141 atcgacggcc gcgaacggct cgtcgagcaa cagcacctgc ggatcgcgtg cgatcgccct
     1201 ggccagtgcg acacgctgct tctggccgcc cgacagctcg tgcgggcggc ggcgctcgag
     1261 cccggcgacg tgcgcgagcg cgatgcactc gccggcgcgc tgccggcgct gccacggcgc
     1321 cacgtgcagc agcgcggtct cgacgttggc ctgcaccgtc aggtgcggaa acagcgcata
     1381 gccctgcggc accaacccga tcgggcgcgc gcgcgtctcg cggtgggtac gctcgtcgtc
     1441 ccatacctcg ccgttgaagg cgatgcgcgc ctgtttcagc cgcagcaggc cggcgatggc
     1501 gcgcaggatc gaggtcttgc cgctgcccga aggtccgacc agcgcggtga tccggccggc
     1561 cggcgcctcg gccacgaagt cgagcggcgg tccgtcgtga tgcagcgcca acgccaggct
     1621 catgtcgctc gccggtgccg catcaggcgc tccgccagac cgtagctgac cgcgatcgtc
     1681 accagcgaga tcgccagcag cagcagcgac atcgcgccgg cggccgcgtg atcgaaggcc
     1741 tgcgcccggt cgtagatcga gatcgccacc gtgcgcgtct cgccggcgat gttgccgccg
     1801 accatcagca ccacgccgaa ttcgccgagc gtgtgcgcga aagtcatcac cgccgccgac
     1861 aggatgcccg gccacgccag cggcagttcg atgcgccaca gcgcctgccg cacgcccagc
     1921 ccgcagcagc gcgcggcctc gtggatctcc tggggaatcg cctcgaacgc gcgctgcatc
     1981 ggctggaccg cgaacggaat gttgaacacc accgaggccg caaccagacc gggaaagctg
     2041 aacgccagcg tgtgtcccga cagcgattcg aaccatcgtc cgagcggcga attgccgccc
     2101 atcgcgacca gcaggtagta gccgatcacc gtcggcggca gcaccagcgg cagcgccagc
     2161 aggccctcga tccaggcctt gccgcgcaca ccggtgtgcg ccagccagcg cgcggcgaac
     2221 agcgccgtgg gcagcagcac cgccagcgtc gccagcgcga gttcgaccga cagcaggatc
     2281 gacggccagt ccatcatggc gcggcctcgc cggcgagcgc gaagccgtgg cgcagcagga
     2341 tcgcgcgcg
//
LOCUS       DAORUQ010000328         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_69058_length_2348_cov_6.080244, whole genome shotgun sequence.
ACCESSION   DAORUQ010000328 DAORUQ010000000
VERSION     DAORUQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69058_length_2348_cov_6.080244"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..633)
                     /locus_tag="PLW13_16165"
     CDS             complement(121..633)
                     /locus_tag="PLW13_16165"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQY71966.1"
                     /translation="MLLSGFPPVARRDARVLILGSMPSEASLAQDFYYAHPANVFWWL
                     LAGVLDCPVPIDVAAKRRLLLGHRIALWDVVHRCRRRGSLDAAIEGESVEANDVGGFL
                     RDHPGITHVFFNGGAAQQLFRRHLGNAVTRDHPRLCFARLPSSSPANASWSRERKLAA
                     WRAVSAALRT"
     gene            complement(636..974)
                     /locus_tag="PLW13_16170"
     CDS             complement(636..974)
                     /locus_tag="PLW13_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71967.1"
                     /translation="MSTAALREAWERELRARLAYLLRSLAAGDDAPPALRFKAEGIAE
                     AGLALGLVDAAELAALLDAIYRECCGAPIAALFSYAAADCIDVARTRVTLPFAMRRAP
                     VYPGPAVETG"
     gene            complement(971..>2348)
                     /locus_tag="PLW13_16175"
     CDS             complement(971..>2348)
                     /locus_tag="PLW13_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY71968.1"
                     /translation="GVCYILDEPTIGLHPRDNTMLLDTLDKLRAKGNTVVVVEHDEET
                     IRRADFVVDLGPGAGRFGGEVVAAGSLAELMASPRSATARFLREPLRHPLRGQRRPVT
                     DDDPCIRVRGATLHNLQRLDAVLPLGRLVCLSGVSGSGKSTLMREVLEPNLRERLARS
                     ATRAWQACTAIEGWESITRVLEVDQAPIGKTPRSCPATYVGIWDAIRKLYAATPEGRV
                     RGFTAGRFSFNTAGGRCESCEGQGRQKVEMSFLPDVTVICEQCGGARFNAETLQVRFR
                     EQRISDVLDMSVADAALFFAAHPAIRRPLELLVDVGLGYLRLGQASPTLSGGEAQRIK
                     LVTELARVRPDDGRRGTSQGQSLYLLDEPTVGLHMADVEKLIEVLHRLVDGGNTVLVI
                     EHNLDVLAEADWLIDLGPEGGAAGGRIVAAGTPEQVARVRGSHTGAALAPFLRERGSS
                     VKRRRGKA"
BASE COUNT          349 a          869 c          817 g          313 t
ORIGIN      
        1 tcgctggcgg ccgaggcgca gaagatacgc gcggccttgc gcgcacacgg acaactcgaa
       61 gcggatttct gagaaacaca tgtccgcatg catgcggaca ttgtccggat gaatccggac
      121 ctacgtgcgc agcgccgcgc tcaccgcgcg ccacgccgcg agcttgcgct cacgcgacca
      181 cgaggcgttg gccgggctgc tcgagggcag gcgcgcgaag cacagccgcg gatgatcgcg
      241 agttaccgcg ttgcccaggt ggcggcggaa cagctgctgc gcggcgccgc cgttgaagaa
      301 cacgtgcgtg atgccgggat ggtcacgcag gaagccgccc acatcgttgg cctcgaccga
      361 ttcgccctcg atcgccgcat ccaggctgcc acggcgccgg cagcggtgaa ccacgtccca
      421 cagcgcgata cggtgcccga gcagcaggcg ccgcttggcg gcgacgtcaa tgggcacagg
      481 acagtcgagc accccggcca gcagccacca gaagacgttg gccggatgcg cgtagtagaa
      541 gtcctgggcc agcgaggcct cgctcggcat cgatccgagg atcagcacgc gcgcatcgcg
      601 gcgcgccacc ggcggaaagc cgctcagcaa cacgctcagc cggtctccac ggcaggcccg
      661 ggatataccg gcgcgcggcg catcgcgaac ggcagcgtca cgcgcgtgcg cgcgacgtcg
      721 atgcagtccg ccgcggcata tgagaacagc gccgcgatcg gtgcgccgca gcactcgcgg
      781 tagatcgcat ccagcagcgc cgcgagctcc gcggcgtcca ccagcccgag tgcgagcccg
      841 gcctcggcga tgccctcggc cttgaagcgc agcgccggcg gcgcatcgtc gcccgcggcc
      901 agcgagcgca gcaggtatgc caggcgcgcg cgcagttcgc gctcccaggc ctcgcgcagc
      961 gccgccgtgc tcatgccttg ccccggcgtc gtttcacgct gctgccgcgc tcgcgcagga
     1021 atggagccag cgcagccccg gtgtgcgagc cgcgcacccg cgccacctgc tcgggcgtgc
     1081 cggcggccac gatgcgcccg ccggccgcgc cgccctcggg ccccagatcg atcagccagt
     1141 ccgcttccgc cagaacgtcg aggttgtgct cgatcaccag caccgtgttg ccgccgtcca
     1201 ccaggcggtg cagcacctcg atcagcttct cgacgtccgc catgtgcagc cccacggtgg
     1261 gctcgtcgag caggtacagc gactgtccct ggctggtgcc gcggcgcccg tcgtcgggcc
     1321 gcacgcgtgc cagttcggtc accagcttga tgcgctgcgc ctcgccgccg ctcagcgtgg
     1381 ggctggcctg tcccaggcgc aggtagccca gacccacgtc gaccagcaac tccagcgggc
     1441 ggcggatcgc gggatgcgcg gcgaagaaca gggcggcatc ggcgacgctc atgtcgagca
     1501 cgtcgctgat gcgctgctcg cggaatcgca cctgcagcgt ctcggcattg aagcgcgcgc
     1561 cgccgcactg ctcgcagatc accgtgacgt cgggcaggaa gctcatctcc accttctgcc
     1621 ggccctgccc ttcgcagctc tcgcagcgcc cgcccgcggt gttgaacgag aagcgccccg
     1681 cggtgaagcc gcgcacgcgt ccctcgggtg tcgcggcgta gagcttgcgg atcgcgtccc
     1741 agatgccgac gtaggtcgcg gggcaggagc gcggggtctt gccgatcggc gcctggtcga
     1801 cctcgagcac gcgcgtgatc gactcccagc cctcgatggc ggtgcaggcc tgccaggcgc
     1861 gcgtggcgct gcgcgccaga cgctcgcgca ggttgggctc cagcacctcg cgcatcagcg
     1921 tgctcttgcc gctgccgctg acgccgctca ggcacaccag ccgacccagc ggcaacaccg
     1981 cgtcgagccg ctgcaggttg tgcagcgtgg cgccgcggac ccggatgcac ggatcgtcgt
     2041 ccgtgaccgg ccggcgctga ccgcgcaggg ggtggcgcaa gggctcgcgc aggaagcgcg
     2101 cggtcgcgga gcgcggcgag gccatcagct cggcgagact gcccgcggcc accacctcgc
     2161 cgccgaagcg cccggcgccg ggcccgagat cgaccacgaa atccgcgcgc cggatcgtct
     2221 cctcgtcgtg ctccaccacc accacggtgt tgcccttggc gcgcagcttg tcgagcgtgt
     2281 cgagcagcat cgtgttgtcg cgcgggtgca ggccgatggt cggctcgtcg aggatgtagc
     2341 acacgccg
//
LOCUS       DAORUQ010000329         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_70945_length_2312_cov_5.243243, whole genome shotgun sequence.
ACCESSION   DAORUQ010000329 DAORUQ010000000
VERSION     DAORUQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70945_length_2312_cov_5.243243"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..604)
                     /locus_tag="PLW13_16180"
     CDS             complement(170..604)
                     /locus_tag="PLW13_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQY71969.1"
                     /translation="MLNVTQILAAKPSGVRTVDVSLTVYAAVTLLASHQIGALVVVDD
                     GRPVGMFSERDYARKGIIRGRRAHKTLVSELMSSPLITVSPEQSIEECMQLMTERRIR
                     HLPVIDDEELVGMVTIGDVVMHMITQQRNHIEQLEGYITGRY"
     gene            complement(641..>2312)
                     /locus_tag="PLW13_16185"
     CDS             complement(641..>2312)
                     /locus_tag="PLW13_16185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY71970.1"
                     /translation="ITAVGEGLAQRGREEIDARGLLVTPGFVDIHTHYDGQVTWENRL
                     VPSTSHGVTTALLGNCGVGFAPCRPADRERLLKLMEGVEDLPEVVLATGLPWNWESFP
                     EYLDALDARRYDCDIATQIPHAALRVFAMGQRAVAREPANAGDCALMARLAGEAIDAG
                     ALGFGTSRTINHRASDGIPVPTLTAAEDELAAIGAALGARGRGVLQLVSDFTDLDTEL
                     PMLRRVVERSGRPLSVSLMQWHHAPDKWRAILAWIDACNADGLDIKAQVSGRPIGLNL
                     GFELSFNPFSYTPTFKSLSGLSVAQRREQLQRPEIRARIVTETPESSGFPGEALLRMW
                     AGIYPLGEQPFYEPVAEGSVAGLAARRGVTPQEMAYDLMLEQDAQAVLMAPTVNFVGN
                     SLDDLLTMMRHPHAIYGLGDGGAHLGFLCDASLPTYMLQYWVRERRRGERLPLAQVVH
                     GLTQRTAQAVGLNDRGVLAPGYKADLNLIDFARLALEPPRVRYDLPAGGRRMVQDARG
                     YVATIVSGAVVQREGKPTGALPGRLVRGEQAVPGQARPVLVTRESYGS"
BASE COUNT          332 a          834 c          788 g          358 t
ORIGIN      
        1 tcacgctggc ggtcggcctg tatccgcagc cgctgatcga cctgctgcag taggttcggc
       61 ttcagagggt gtttgcaaaa cgtcttccgg ccacgcggac acgctgtttt tcgggtgtag
      121 gagcggccca tggccgcgat atcgcgcgca tggcgcgctc ctacgacgct cagtagcgtc
      181 cggtgatata gccttccagc tgctcgatgt ggttgcgctg ctgcgtgatc atgtgcatca
      241 ccacgtcgcc gatggtcacc atgccgacga gctcctcgtc gtcgatgacc ggcaggtggc
      301 ggatgcgccg ctccgtcatc agctgcatgc actcctcgat cgactgttcc ggagacacgg
      361 tgatcagcgg cgagctcatc agctcgctca ccagcgtctt gtgcgcccgg cgcccccgga
      421 tgatgccctt gcgcgcgtag tcgcgttccg aaaacatgcc gaccgggcgg ccgtcgtcga
      481 ccaccacgag cgcgccgatc tggtggctcg ccagcagcgt gaccgccgca tagaccgtga
      541 gcgaaacgtc cacggtccgc acgccggagg gtttggccgc caggatctgg gtcacgttga
      601 gcatggactc ccccgccgaa tcttgcgtaa agcgccgata tcatgaccca tacgattcgc
      661 gggtgacaag cacggggcgc gcctgtcccg gcactgcctg ctctccccgg accaggcgtc
      721 ccggcagcgc gccggtgggc tttccctcgc gctgcaccac cgccccgctg acgatggtcg
      781 cgacataacc gcgcgcatcc tgcaccatgc gccgcccgcc ggccggcagg tcgtagcgca
      841 cgcgcggcgg ctcgagcgcg aggcgcgcga aatcgatgag gttgaggtcg gccttgtagc
      901 ccggcgccag cacgccgcga tcgttcagcc ccaccgcctg cgcggtacgc tgcgtcagtc
      961 cgtgcaccac ctgcgccagc ggcaggcgct cgccgcgcct gcgctcgcgc acccagtact
     1021 gcagcatgta ggtcggcagg ctcgcgtcgc acaggaaccc gaggtgcgcc ccgccatcgc
     1081 cgagaccata gatcgcgtgc gggtggcgca tcatcgtgag caggtcgtcc agcgagttgc
     1141 ccacgaagtt cacggtgggc gccatcagca cggcctgcgc gtcctgctcg agcatcaggt
     1201 cataggccat ttcctgcggc gtcacgccgc ggcgcgcggc caggcccgcg acgctgccct
     1261 cggccacggg ctcgtagaag ggctgttcgc cgagcgggta gatgcccgcc cacatccgca
     1321 gcagcgcctc tcccgggaag ccggaggact cgggcgtctc ggtcacgatg cgcgcgcgga
     1381 tctcggggcg ctgcaactgc tcgcggcgct gcgccacgct cagcccggac agcgacttga
     1441 aggtcggcgt gtagctgaac gggttgaagc tcagctcgaa gccgaggttc agcccgatcg
     1501 ggcgcccgct gacctgggcc ttgatgtcca gcccgtcggc attgcaggcg tcgatccagg
     1561 cgaggatcgc gcgccacttg tcgggcgcgt ggtgccactg catcagggac accgacagcg
     1621 gccggcccga gcgctccacg acgcggcgca gcatcggcag ctcggtgtcc aggtcggtga
     1681 aatcggagac cagctgcagc acgccgcgcc ccctggcgcc cagcgcggca cctatggccg
     1741 cgagctcgtc ctcggccgcg gtcagcgtgg gcaccgggat gccgtcgctg gcgcggtggt
     1801 tgatcgtgcg cgaggtgccg aagcccagcg ccccggcatc gatcgcctcg cccgccagcc
     1861 gtgccatcag cgcgcagtcc ccggcgttgg ccggctcgcg cgccaccgcg cgctgcccca
     1921 tcgcgaagac gcgcagcgcc gcgtgcggga tctgggtcgc gatgtcgcag tcgtagcgtc
     1981 gcgcgtccag cgcgtccagg tactcgggga agctctccca gttccacggc agcccggtgg
     2041 cgagcaccac ctcgggcagg tcctcgacgc cttccatgag cttcagcagc cgctcgcggt
     2101 ccgcggggcg gcagggcgcg aagccgacgc cgcaattgcc gagcagcgcg gtggtgacgc
     2161 cgtgcgaggt cgagggcacc aggcggttct cccaggtgac ctggccgtcg tagtgcgtgt
     2221 ggatatcgac gaaaccgggc gtgaccagca ggccgcgcgc gtcgatctcc tcgcgcccgc
     2281 gctgcgcgag cccctcgccc accgcggtga tc
//
LOCUS       DAORUQ010000330         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_71315_length_2305_cov_12.282667, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000330 DAORUQ010000000
VERSION     DAORUQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71315_length_2305_cov_12.282667"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1209)
                     /locus_tag="PLW13_16190"
     CDS             complement(238..1209)
                     /locus_tag="PLW13_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71971.1"
                     /translation="MNAIHTLATTLSTGTPQTAAGLTLVPLLRRTPARLAYLGFAEAL
                     KRSLVEVTELGPGGSVPEVSVLNRGELPVLFLDGEALVGAKQNRVLNLSVLVPGKATT
                     TIPVSCVEAGRWSPRSRAFVDGESLHFAKARACKVANVSEAMAQGMGRRSNQGDVWAT
                     MDGYLADFDAAAPSRAMQDGYAKVQHTVDDLLGKLPALDGQAGAAFLLHGKLLGIDLM
                     GSAEAFAEIHAKVVRSYAVEALRGGPSASSDPGAAPAGGVADDIAAESVRQALAELAT
                     ASWQQHPAIGLGTDLRLSAPGWDAAALVVDEELVHLAAFPRTRLASP"
     gene            complement(1272..1922)
                     /locus_tag="PLW13_16195"
     CDS             complement(1272..1922)
                     /locus_tag="PLW13_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71972.1"
                     /translation="MPDRFLSYLIEGEREGLVLPPGAIAAHERAQALGCVAAVQLLAE
                     GAPIAIDIAMRARRLLRVLERSGPSPRPYAVLREPGIAGRFADDDQLALPSDPIEVAV
                     EAGMQAIADAPRLHVDLRADAQPRSVDAGGIGLTLRTLAGHSGAMPWLELELLDAGSR
                     QLEGAAALLWSQPGDLQRSPFDLAGVARLRWPAAPRNTLRIDVGPRAALIDLHATT"
     gene            complement(1958..>2305)
                     /locus_tag="PLW13_16200"
     CDS             complement(1958..>2305)
                     /locus_tag="PLW13_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71973.1"
                     /translation="DLLRGLRRETGFDDEQEFHADPEGLFEEGVHNDPELDAELALRR
                     RLARACLDALPSGTRAALRERIRGRPDDLAARLLHIAGGAFRQRISSGMRLLAACVAQ
                     GGPDPEPADRPGR"
BASE COUNT          319 a          813 c          839 g          334 t
ORIGIN      
        1 cacagtagcc ctcgatcttg ccccgctcct tgacgcagcg ggagccgggc tcgcagtcgg
       61 tgtcgaagct gcaggcgctg gccatcggtg cggccgcgag gagcaggacg atggcaagtg
      121 cgattcgggt tgccgtgtac acgagatcac tccggtgtcg cgggttgggc cggcggagcg
      181 ccgcgtgggc ggcgctccgc aacggatcga tgtcacgcgc gtagcgtgcg ggtttgctca
      241 gggcgacgcc agccgcgtgc gtggaaacgc cgcgaggtgc accagttcct cgtcgacgac
      301 cagcgccgcg gcgtcccagc ccggcgcgga caggcgcagg tcggtgccga gtccgatcgc
      361 ggggtgttgc tgccacgatg cggtggccag ctcggcgagc gcctggcgca cggattccgc
      421 ggcaatgtcg tccgcaacac caccagcagg agcggccccc ggatcgctcg atgcgctggg
      481 cccgccgcgc agggcctcga cggcgtagct gcgcacgacc ttcgcgtgga tttccgcgaa
      541 ggcctcggcg ctgcccatga ggtcgatgcc gagcaacttg ccgtggagca ggaacgctgc
      601 gccggcctgc ccgtccagcg cgggcagctt gccgagcagg tcgtccaccg tgtgctggac
      661 cttggcgtag ccgtcctgca ttgcgcgact gggcgccgcg gcatcgaagt cggccaggta
      721 gccgtccatc gtggcccaga catcgccctg gttcgagcga cgacccatgc cctgtgccat
      781 cgcctcggaa acgttcgcca ccttgcacgc gcgggccttg gcgaaatgca gtgactcgcc
      841 gtcgacgaag gcgcggctgc gcggcgacca gcgcccggcc tcgacgcagg acaccgggat
      901 cgtcgtcgtg gccttgcctg gcaccagcac cgagaggttc agcacgcgat tctgcttcgc
      961 gcccaccaag gcctcgccat cgaggaacag gacgggcagc tcgccccggt tgagcaccga
     1021 gacctccggg acgctgccgc cgggcccgag ttcggtcact tcgacgaggc tgcgcttcag
     1081 tgcctcggcg aagcccaggt acgccaacct ggcgggcgtg cgccgcaaca gcggcaccag
     1141 ggtcaggccg gccgccgttt gcggcgtacc cgtggacagc gtcgtggcca gcgtatggat
     1201 cgcgttcatg tcgcctccgt gggggcccca tggcccccct gcctctcttg acgtggcggc
     1261 gacgcgagtt gtcaggtcgt ggcgtgcagg tcgatcaggg ccgcgcgcgg gccgacatcg
     1321 atgcgtaggg tgttgcgtgg cgcggcgggc cagcgcagtc gcgccacgcc cgcgaggtcg
     1381 aaggggctgc gttggaggtc gccgggctgg ctccacagga gggccgccgc gccttccagt
     1441 tgccggctcc cggcatcgag gagttccagt tcaagccacg gcatggcgcc ggaatggccg
     1501 gcaagcgtgc ggagcgtcaa gccgatgcca ccggcgtcta ccgatcgtgg ctgcgcgtcg
     1561 gcgcgcaggt ccacgtgcag tcgcggcgca tccgcgatgg cctgcatgcc ggcctcgacc
     1621 gcgacttcga tcggatcgga cgggagcgcg agctggtcgt catcggcgaa gcgtccggct
     1681 atgccgggct cgcgcaggac cgcgtagggt ctcggagacg gcccggatcg ctccagcacg
     1741 cgcaggaggc gccgtgcccg catcgcgatg tcgatggcaa tcggcgcgcc ttcggccagg
     1801 agctgcaccg cggccacgca gcccagcgcc tgggcgcgct catgcgccgc gatggcgccg
     1861 ggcggcagca ccaagccctc gcgctcgccc tcgatgaggt aggacaggaa tcgatccggc
     1921 atgggtccca tcgtcgcctc tccctgatcc tgacgtctca gcggcccggt ctgtcagccg
     1981 gctccggatc cggcccgccc tgcgcgacgc aggcggcgag caggcgcatg ccactgctga
     2041 tccgctggcg aaaggcgccg ccggcgatgt gcagcaggcg cgcggccaga tcgtccggtc
     2101 gcccgcggat gcgctcgcgc agcgcggccc gggtgccgga gggcagcgca tcgaggcagg
     2161 cgcgggcgag gcgccgccgc aaggccaact cggcatccag ctcgggatcg ttgtgcacgc
     2221 cctcctcgaa caggccctcc gggtcggcgt ggaattcctg ctcgtcatcg aatcccgttt
     2281 cacgtcgcag cccgcgcagc aggtc
//
LOCUS       DAORUQ010000331         2286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_72369_length_2286_cov_4.359480, whole genome shotgun sequence.
ACCESSION   DAORUQ010000331 DAORUQ010000000
VERSION     DAORUQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72369_length_2286_cov_4.359480"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLW13_16205"
     CDS             complement(<1..490)
                     /locus_tag="PLW13_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY71974.1"
                     /translation="MSTIVQIALEDGILELRLNRPERLNALSEELIGELTDILRGLNR
                     NREVRVAIITGEGKGFCAGADLKADSAPGAIPGTEGMSHLGYVYKYQEYLADLMLAIH
                     ECDKPVIAAINGAAVGGGFAFALASDIRIASEKARFGAVFIKTGLSSCDVGTSYFLPR
                     LVG"
     gene            complement(524..1594)
                     /locus_tag="PLW13_16210"
     CDS             complement(524..1594)
                     /locus_tag="PLW13_16210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71975.1"
                     /translation="MDFSLNEIQQDIASLAEQILRGASTPERLKALEADGQRLDRPLW
                     AELVAAGLTAVAIPEARGGAGLGFFETCLVLEQVGRTVAAVPLAGHGVGLMALHHAGA
                     DAALDRLLAGHGWLATSARTDAGNKLRAESGRLRGELGAVPFADGSQYLLLPARRDGA
                     WQLFLLDTAQPGVRFEAQLTTALEPRARVQLDGVAAAAIGDGELVEWLRERIIAATCA
                     VQTGVLGEMIAMSTRYVSEREQFGAKIGSFQSVSHRMADTWIDLMNLRLLGQSAAAML
                     DRQPLATLEVLAAQSWCAEAGHRVSASCQHVHGGMGHDRDYPLWRYAVWSRHNELVHG
                     GASETYAAIGAAIARVPASAAL"
     gene            complement(1609..>2286)
                     /locus_tag="PLW13_16215"
     CDS             complement(1609..>2286)
                     /locus_tag="PLW13_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY71976.1"
                     /translation="LAVRTGDPATHPRHKGISMLLVPTDSPGFSRTPIHTVADGETNT
                     TYYDNVRVPASHLIGAENGGWQVITSQLNRERLTLMNPGTASLLYTQVLEYVQAHRDE
                     SGRRLIELPWVQGALGRIYAGMRVLHLACRKQAWAIAQGNPHPAEASAVKVYGYECFL
                     QCYRLMMEVLGPDAVLRRGSAGALLEGLPEQIYRAVPVYGFGGGANEIQRDLIAQFGL
                     GLQRAKR"
BASE COUNT          338 a          826 c          773 g          349 t
ORIGIN      
        1 cgccgaccag gcgcggcagg aaatagctcg tgcccacgtc gcaggacgag agcccggtct
       61 tgatgaacac ggcgccgaag cgcgccttct cgctggcgat gcgaatgtcg ctcgccagcg
      121 cgaaggcgaa gccgccgccc accgccgcgc cgttgatcgc ggcgatcacc ggcttgtcgc
      181 actcgtggat cgcgagcatc agatcggcga gatactcctg gtacttgtag acgtagccca
      241 gatggctcat gccctcggtg ccgggaatcg cgcccggagc ggagtccgcc ttgaggtccg
      301 cgccggcgca gaaacccttg ccctccccgg tgatgatcgc gacacgcacc tcgcggttgc
      361 ggttcagccc gcgcaggata tcggtcagct ccccgatcag ttcctcgctc agcgcgttga
      421 gccgctccgg ccggttcagg cgcagctcga ggatgccgtc ttcgagcgcg atctgcacga
      481 tcgtggacat gaggcctctc cgtggttgca gttacgatcg ggttcagagc gcggccgagg
      541 ccggcacgcg cgcgatggcg gcgccgatgg cggcataggt ctcgctggca ccgccatgca
      601 ccagctcgtt gtgacgggac cagactgcgt agcgccacag cgggtagtcg cggtcatggc
      661 ccatgccgcc atgcacgtgc tggcagctcg ccgagacacg gtgtccggcc tcggcacacc
      721 agctctgcgc cgccagcacc tccagcgtgg cgagcggctg gcgatcgagc atcgcggcgg
      781 ccgactggcc gagcaggcgc aggttcatca ggtcgatcca ggtgtccgcc atgcggtgac
      841 tcaccgactg gaaactgccg atcttcgcac cgaactgctc gcgctcggac acataacgcg
      901 tgctcatcgc gatcatctca ccgagcacgc cggtctgcac cgcgcaggtc gcggcgatga
      961 tccgctcgcg cagccactcc accagttccc catcgccgat cgccgcggcg gccacgccat
     1021 cgagctgcac gcgcgcgcgt ggctcgaggg ccgtggtgag ctgcgcttcg aagcgcacgc
     1081 ccggctgcgc cgtatcgagc aggaacagct gccatgcccc gtcgcggcgc gccggcagca
     1141 gcaggtactg gctgccgtcg gcgaagggca ccgcgcccag ttcgccccgc aggcgcccgc
     1201 tctcggcgcg cagcttgttg cccgcatcgg tgcgggcgct ggtggcaagc cagccgtgcc
     1261 ccgccagcag gcgatcgaga gccgcgtcgg ctccggcgtg gtgcagcgcc atcaggccga
     1321 caccgtggcc ggcgagcggc accgcggcca cggtcctgcc cacctgctcc agcaccaggc
     1381 aggtctcgaa aaagcccagc cccgccccgc cgcgcgcctc cggaatcgcg acggccgtga
     1441 gccccgcggc caccagctcc gcccacagtg ggcggtcgag gcgctggccg tccgcctcca
     1501 gcgccttcag ccgttcgggc gtcgaggcgc cgcgcaggat ctgttccgcc agggaagcga
     1561 tgtcctgctg gatctcgttc agcgaaaaat ccatggcctt gccgccgctc agcgtttggc
     1621 gcgctgcaga ccgagcccga actgggcgat caggtcgcgc tggatttcat tggcgccgcc
     1681 cccgaagccg tagaccggca cggcgcggta gatctgctcc ggcaggccct cgagcagcgc
     1741 gccggccgag ccgcgtcgca gcaccgcatc cggtccgagc acttccatca tcaggcggta
     1801 gcactgcagg aagcactcgt agccgtagac cttgaccgcc gatgcctcgg ccgggtgcgg
     1861 attgccctgc gcgatggccc atgcctgctt gcggcaggcg aggtgcagca cgcgcatccc
     1921 ggcatagatg cgtcccaacg cgccctgtac ccacggcagc tcgatcaggc gccgtcccga
     1981 ctcgtcgcgg tgcgcctgca cgtactcgag tacctgcgtg tacagcaggc tcgcggttcc
     2041 cgggttcatc agcgtcaggc gctcgcggtt cagctggctg gtgatcacct gccagccgcc
     2101 gttctccgcg ccgatcaggt ggctcgcagg cacgcgcaca ttgtcgtagt aggtggtgtt
     2161 ggtctcgccg tccgccaccg tgtggatcgg ggtgcgcgag aagccggggc tgtcggtggg
     2221 caccagcagc atcgagatgc ccttgtggcg cgggtgagtc gcgggatcgc cggtgcgaac
     2281 cgccag
//
LOCUS       DAORUQ010000332         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_74133_length_2253_cov_6.131483, whole genome shotgun sequence.
ACCESSION   DAORUQ010000332 DAORUQ010000000
VERSION     DAORUQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74133_length_2253_cov_6.131483"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLW13_16220"
     CDS             <1..268
                     /locus_tag="PLW13_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY71977.1"
                     /translation="PREHTRAELEWQHMGRYFLEPTETHDYEGHELLHLRLNQQLSRH
                     FSWSVRVTNLTDEQYADRADFAFGDYRYFIGEPRSLFVDVGWQL"
     gene            288..950
                     /locus_tag="PLW13_16225"
     CDS             288..950
                     /locus_tag="PLW13_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQY71978.1"
                     /translation="MTLKVELYWSFRSPYSYLATPRVCALAREYDIAFDVRIVLPLAV
                     RKPEFFRDVNPLWPPYVLRDTVRLAQMLGLDYAWPDPDPIVQDFATRTIAAEQPHIHR
                     ISRLGVEASRQGRGLEFIDEASRLIWSGAVRGWNTGEHLAGAAARAGLDLATMERNIA
                     ARMPWHDAALAANQQALEAAGHWGVPTFVFQGEPFFGQDRIDVLLWRLGQHGLARRAG
                     AP"
     gene            953..2035
                     /gene="alr"
                     /locus_tag="PLW13_16230"
     CDS             953..2035
                     /gene="alr"
                     /locus_tag="PLW13_16230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQY71979.1"
                     /translation="MARPTRALIDLAALRHNCALAQSLAPGASTLAVVKSNAYGHGAP
                     AVARALEGVVQAFGVACIEEALELREAGVRAPVLLMEGVFEPVELDIAARENFWLMVE
                     SPRQLEWLAAARPVAPLGVWLKVDTGMGRLGFAPAAARAAWASLSANPAVAPGIVVAT
                     HMACADELENGATTRQLTRLREALGDIDAPLSIANSPALLGWPAARAQWNRLGYMLYG
                     CSPFATPHAVAAALRPVMTLVSEVISLHDLAAGESAGYGAIWTATRPSRLATIPAGYT
                     DGYPRSAANGTPVLVDGRIAPLAGRVSMDMLMVDVTDLPQVKPGDPVQLWGPDLNVDT
                     VAAHCGTIGYELLVRIPPRVPRVYRP"
BASE COUNT          317 a          850 c          757 g          329 t
ORIGIN      
        1 gccgcgcgag cacactaggg ccgagctcga gtggcagcac atgggccgct acttcctcga
       61 gcccaccgag acgcatgact acgagggcca cgagctgctg cacctgcgcc tgaaccagca
      121 gctctcgcgc catttctcgt ggtcggtgcg cgtgaccaat ctcaccgacg agcagtacgc
      181 cgatcgcgcc gacttcgcct tcggcgacta ccgctacttc atcggcgagc cgcgcagcct
      241 gttcgtcgac gtcggctggc aactctgaaa cccgaggcaa cgcgaacatg acgctgaaag
      301 tcgaactcta ctggtcgttc cgcagcccct actcctacct cgcgacaccg cgcgtgtgcg
      361 cgctggcgcg cgagtacgac atcgccttcg atgtgcgcat cgtgctgccg ctggcggtgc
      421 gcaagcccga gttcttccgc gacgtgaacc cgctgtggcc gccctacgtg ctgcgcgaca
      481 cggtgcgcct ggcgcagatg ctcggtctcg attacgcctg gcccgacccg gatcccatcg
      541 tgcaggattt cgccacgcgc accatcgccg ccgagcagcc gcacatccac cgcatcagcc
      601 gcctcggcgt cgaggccagc cgccaggggc gcggcctcga attcatcgac gaggcctcgc
      661 gcctcatctg gagcggcgcg gtgcgcggct ggaacaccgg cgagcacctc gcgggcgccg
      721 cggcgcgcgc cgggctcgat ctcgcgacga tggagcgcaa catcgccgcg cgcatgccgt
      781 ggcacgacgc cgcgctcgcc gccaaccagc aggcgctcga ggctgccggg cactggggcg
      841 tgccgacctt cgtgttccag ggcgagccct tcttcggcca ggatcgcatc gacgtgctgc
      901 tgtggcgcct gggccagcac ggtctcgcgc gccgcgccgg cgcgccctga caatggcccg
      961 ccccacccgc gcgctcatcg acctggcggc gctgcgccac aactgcgcgc tggcgcagtc
     1021 gctcgcgccc ggcgccagca cgctcgcggt ggtgaagtcg aatgcctacg gacacggcgc
     1081 gcccgcggtg gcgcgcgcgc tcgagggcgt ggtgcaggct ttcggcgtgg cctgcatcga
     1141 ggaggcgctg gagctgcgcg aggccggcgt gcgcgcgccg gtgctgctga tggaaggcgt
     1201 gttcgagccc gtggagctcg acatcgcggc gcgcgagaac ttctggctga tggtcgaaag
     1261 cccgcgccag ctcgagtggc tcgccgcggc acgcccggtg gcgccgctcg gcgtgtggct
     1321 gaaggtggac accggcatgg gccggctcgg tttcgccccc gcggccgcgc gcgcggcttg
     1381 ggccagcctg tccgcgaacc ccgcggtggc tccgggcatc gtggtggcca cgcacatggc
     1441 ctgcgccgac gagctcgaga acggcgccac cacgcgccag ctcacgcgcc tgcgcgaagc
     1501 cctgggcgac atcgacgcgc cgctcagcat cgccaactcc cccgcgctgc tcggctggcc
     1561 cgcggcgcgc gcgcagtgga accgcctcgg ctacatgctc tacggctgct cgcccttcgc
     1621 cacaccgcac gcggtggccg ccgcactgcg accggtgatg acgctggtct ccgaggtgat
     1681 ctcgctgcac gacctggctg ccggggaatc cgccggctac ggcgcgatct ggaccgcgac
     1741 ccggccctcg cgtctcgcga ccatcccggc gggctacacc gacggctatc cgcgcagcgc
     1801 cgcgaacggc acgccggtgc tggtggacgg ccgcatcgcg ccgctcgcgg gacgcgtgtc
     1861 gatggacatg ctgatggtcg atgtcaccga cctgccacag gtcaagcccg gcgatccggt
     1921 gcagctgtgg ggccccgacc tgaacgtgga cacggtggcc gcgcactgcg gcaccatcgg
     1981 ctacgagctg ctggtgcgca tcccgccgcg cgtaccgcgc gtttatcgcc cgtagggcgc
     2041 acaaaatccg aggcccctcg cgttccgcga ttttttctat agaaatccgc tgattacgag
     2101 ccctcgcgga acagcctaac gcgagttgcc gccaccccct gcgccgcatt atggtgcgcg
     2161 catgcccgat aacgacaaca gcatcagcgc aacccgccgc cagcccaggc aacgccgctc
     2221 gcgcctgctg gtggcctcgc tgcgcgaggc ctg
//
LOCUS       DAORUQ010000333         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_74251_length_2251_cov_9.761840, whole genome shotgun sequence.
ACCESSION   DAORUQ010000333 DAORUQ010000000
VERSION     DAORUQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74251_length_2251_cov_9.761840"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..292
                     /locus_tag="PLW13_16235"
     CDS             2..292
                     /locus_tag="PLW13_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HQY71980.1"
                     /translation="MDLEAVLRSRLLWDAGEPSPEALASTQPFCIDTLDFPQWLQFVF
                     LPRMQALLDADLPLPAQCAVAEMAEMHFGAAEAADVIALLREIDLAVVAASP"
     gene            677..1534
                     /locus_tag="PLW13_16240"
     CDS             677..1534
                     /locus_tag="PLW13_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY71981.1"
                     /translation="MQDFSGKVAFVTGGDKGMGLVTARQLAARGAHVAIFSRQPGEAA
                     EQEIRTACRSPAQQVMRFALDVANRDNVLEIMARAQRAMRAPDLVICMAGIGATRRFV
                     DMPFEVFDRMLQVNLYGTRHVIEAVLPGMIARGRGTLVPVASLGGFVPVYGYTAYGTS
                     KFAVLGLAQCLRYELAPLGIEVCCFCPGQVATPGLAAEAQTLLPAAGALKLAGGTLPV
                     EPAIRGLLRGIERGRPVIIPGARANLVYWLWRLTPIRLWNAITDAIVRVALRPASPSA
                     DGGAGPGPR"
     gene            complement(1597..>2251)
                     /locus_tag="PLW13_16245"
     CDS             complement(1597..>2251)
                     /locus_tag="PLW13_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY71982.1"
                     /translation="GIVVIREQILARTQGPMRQGIELAVGVLYRTVREFLGPGWSPLA
                     VCFMHEPPRDLAPYRRAFGTGVQFNSVLNGLTCRASELDRAMPMADPQSLRTIRQYLE
                     SAARRRPTQSERTAQLILGLLPSGRCSAELVARYIGIDRRTLHRRLAREGTSFSALVD
                     ALRREAAQRHLGNPQQSVADLAVMLGFSGSSAFSRWFCASFGCAPRRWRRQATQAKT"
BASE COUNT          328 a          786 c          805 g          332 t
ORIGIN      
        1 gatcgacctc gaggcggtgc tgcgcagccg cctgctctgg gacgccggcg agccctcgcc
       61 cgaagcgctg gccagcaccc agccgttctg catcgacacg ctcgatttcc cgcagtggct
      121 gcagttcgtg ttcctgccgc gcatgcaagc actgctcgat gcggacctgc cgctgcccgc
      181 gcaatgcgcc gtcgccgaaa tggcggaaat gcacttcggc gccgcagagg cggccgacgt
      241 gatcgcattg ctgcgcgaga tcgacctcgc cgtcgtcgcc gcgtccccgt aggcccggac
      301 gtgggtccgg atccagaggg tgtcgcaaaa gccatcaggt tgcgagcgag cggcctggtg
      361 cgtcgccggc tgcgctgccc gccattgtcc tgcggggcac gcacgagtac gtccctgtag
      421 ctccgcgccg cgtcctgcgg cgcaaggccc cgcaagccaa cagcgggcag cacagccgct
      481 cggttgagcg cgtgtcgcat cgggcagatc cgggaatgcg ccgcggcacg ggtcgccaga
      541 cgggcgatgc aaaatcgggc cctggtgctg ccgtccggct ccagggatag gatgtcggac
      601 gcgctggcaa ggcgggcggg cagggcacgg tgtcatgctg tgcgtcttct acacggcgcc
      661 gcggaacgca aaccacatgc aggacttctc gggaaaggtc gctttcgtca ccggcggcga
      721 caagggcatg ggcctcgtca cggcgcgcca gcttgcggcg cgcggtgcgc acgtggcgat
      781 cttctcgcgt cagcctggcg aggccgcgga gcaggagatc cgcaccgcct gccgctcacc
      841 ggcccagcaa gtgatgcgtt tcgcgctcga cgtcgcgaac cgcgacaacg tgctcgagat
      901 catggcccgg gcacaacggg cgatgcgcgc accggacctc gtgatctgca tggccggcat
      961 cggcgccacc cggcgattcg tcgacatgcc gttcgaggtg ttcgatcgca tgctgcaggt
     1021 gaacctctac ggcacgcgtc acgtgatcga ggccgtgctg cccggcatga tcgcgcgcgg
     1081 ccgcggcacg ctggtgcccg tggcttccct gggaggcttc gtgcccgtgt acggctacac
     1141 cgcttacggg acctcgaagt tcgcggtgct gggcctcgca cagtgcctgc gctacgagct
     1201 cgcgccgctg ggaatcgagg tttgctgctt ctgtccgggg caggtcgcga cgccgggact
     1261 ggccgccgag gcgcagaccc tgctgcccgc ggccggggcg ctcaagctgg caggcggcac
     1321 gctgccggtg gagcccgcaa tccgcgggct gctgcggggc atcgagcgcg gccgccccgt
     1381 gatcatcccc ggcgcacgcg cgaacctggt gtactggctt tggcgcctga cgccgatacg
     1441 cctctggaac gccatcacgg acgcgatcgt gcgcgtcgcg ctgcgtcccg cctcaccgag
     1501 cgcggacggc ggggcgggcc cagggccgcg ctgagtttgc tgtcggcccg gcgatcgacg
     1561 acgcgaacgc ccgagcggaa gcgcatccgc ttcctgtcag gtcttcgcct gcgtggcctg
     1621 gcgacgccag cgccgcggcg cgcagccgaa gctcgcgcag aaccagcgcg agaaggcgct
     1681 ggaaccggaa aagccgagca tgaccgcgag atcggctaca gattgctgcg gattgcccag
     1741 atggcgttgc gcggcctccc ggcgcaacgc gtcgaccagt gcggagaaac tcgtgccttc
     1801 gcgcgccagc cgccggtgca gcgtgcgccg gtcgatgccg atatagcgcg cgacgagctc
     1861 cgcgctgcag cgcccgctcg gcagcagtcc gaggatcaac tgcgcggtgc gctcgctttg
     1921 ggtgggccgg cgccgggcgg cgctctccag gtattggcga atcgtgcgta gcgactgcgg
     1981 atccgccatc ggcatggccc gatccagctc gctggcgcgg caggtcagcc cgttcagcac
     2041 gctgttgaac tgcacgccgg taccgaaggc ccggcggtag ggcgcgagat cgcgcggcgg
     2101 ctcgtgcatg aaacacacgg ccagcggcga ccagcccggg ccgaggaatt cccggacggt
     2161 gcgatacagc acgccgaccg cgagctcgat cccctggcgc atcggaccct gcgtgcgcgc
     2221 gaggatctgc tcgcggatca cgacgatgcc g
//
LOCUS       DAORUQ010000334         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_74902_length_2239_cov_5.732601, whole genome shotgun sequence.
ACCESSION   DAORUQ010000334 DAORUQ010000000
VERSION     DAORUQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74902_length_2239_cov_5.732601"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="PLW13_16250"
     CDS             <1..1736
                     /locus_tag="PLW13_16250"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HQY71983.1"
                     /translation="VIPEDGGATRAGPYNPVRGARVIAFARAFLDRAAPLASGSHADA
                     TAYAVRDGVLMVTLGGAPVRLRMPAQFAGYRGSPDAPDAVLLRNHGLHIEIQIDRAHP
                     IGKDDAAGVKDLLLESALTTIQDCEDSIAAVDAEDKVEVYRHWLGLMKGDLVETFGKG
                     GRTLTRTMNPDREYTAPDGSTLSLQGRSLLLVRNVGHLMSTDAVLDENGAEIPEGILD
                     GMVTVLAAMHDLRRTSGPRNSRAGSVYIVKPKMHGPEEVALACDLFGAIEGVLGLARN
                     TLKIGIMDEERRTTLNLKECIRVARERVIFINTGFLDRTGDEIHTSMEAGPMVRKDAM
                     KNQTWIQAYEDWNVDNGLATGFSGRAQVGKGMWAKPDEMAQMLKTKIAHPQAGASCAW
                     VPSPTAATLHVMHYHEVDVAARQRELMGRPRANRDDILRIPLLTDRASLSATDIQQEL
                     DNNAQGILGYVVRWIDQGVGCSKVPDINNVGLMEDRATLRISSQHIANWLRHGICSEA
                     QVMATLRRMAAVVDAQNAGDPLYRNMATDLDASIAFRAACDLVFKGCAQPNGYTEPLL
                     HAHRRAAKARA"
     gene            complement(1776..>2239)
                     /locus_tag="PLW13_16255"
     CDS             complement(1776..>2239)
                     /locus_tag="PLW13_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HQY71984.1"
                     /translation="DEYGNAIKQMAAANIFPGDMLLKNFGVTRHGRVVFYDYDEICPL
                     TECNFRRIPEPRDETEEMAGTPWYTVAENDVFPEEFRLFFSGNPHAKAAFEAQHADLF
                     DYRYWQKLQAAIRAGYVFDTFPYRRRARFPREDDDSAFTYDMAIRAGDIPS"
BASE COUNT          396 a          765 c          759 g          319 t
ORIGIN      
        1 acgtgatccc cgaggacggc ggagccacgc gcgccggccc ctacaacccg gtgcggggcg
       61 cccgcgtcat cgctttcgcg cgcgcgttcc tcgaccgcgc ggcaccgctc gcctcgggca
      121 gccacgccga tgccactgcc tatgcggtgc gagacggcgt attgatggtc acgctcggcg
      181 gcgcgccggt gcgcctgcgc atgccggcgc agtttgccgg ctaccggggc agccccgacg
      241 cgccggatgc ggtgctgctg cgcaatcacg ggctgcacat cgagatccag atcgaccgcg
      301 cgcacccgat cggcaaggac gacgcggccg gcgtgaagga cctgctgctc gaatcggcgc
      361 tgaccacgat ccaggactgc gaggactcca ttgccgccgt ggacgccgag gacaaggtcg
      421 aggtctaccg ccactggctg gggctcatga agggcgacct cgtcgagacc ttcgggaagg
      481 gcggacgcac gctgacgcgc accatgaacc ccgaccgcga atacaccgcg cccgacggct
      541 ccacgctttc cctgcaggga cgctcgctgc tgctggtgcg caacgtcggc cacctgatga
      601 gcaccgacgc ggtgctcgac gagaacggcg cggagatccc cgaaggcatc ctcgacggca
      661 tggtgacggt gctggcggcg atgcacgacc tcaggcgcac tagtggcccg cgcaattcgc
      721 gcgccggcag cgtctacatc gtgaagccga agatgcacgg tcccgaggaa gtcgcgctgg
      781 cctgtgatct tttcggcgcg atcgaggggg tgctgggact cgcgcgcaac acgctcaaga
      841 tcggcatcat ggacgaggaa cgccgcacca cgctgaacct gaaggagtgc atccgcgtcg
      901 cgcgcgagcg cgtgatcttc atcaacaccg gcttcctcga ccgcaccggc gacgagatcc
      961 acaccagcat ggaagccggc ccgatggtgc gcaaggacgc catgaagaac cagacctgga
     1021 tccaggccta cgaggactgg aacgtcgaca acgggctggc caccggcttc agcggccgcg
     1081 cacaggtcgg caagggcatg tgggccaagc ccgacgagat ggcgcagatg ctgaagacca
     1141 agatcgcgca cccgcaggca ggcgcgagct gcgcctgggt accctcgccg accgcggcca
     1201 cgctgcacgt gatgcactac cacgaggtcg atgtcgcggc gcgccagcgc gagctcatgg
     1261 gccgcccgcg cgcgaaccgc gacgacatcc tgcgcatccc gctgctcacg gatcgcgcca
     1321 gcctttcggc caccgacatc cagcaggaac tcgacaacaa cgcccagggc atcctcggct
     1381 acgtggtgcg ctggatcgac cagggcgtgg gctgctcgaa ggtgcccgac atcaacaacg
     1441 tgggcctgat ggaggaccgc gcgacgctgc gcatctcgag ccagcacatc gcgaactggc
     1501 tgcgccacgg catctgcagc gaagcgcagg taatggcgac gttgcggcgc atggccgcgg
     1561 tggtggacgc ccagaacgcg ggggacccgc tgtatcgcaa catggccacg gacctcgacg
     1621 ccagcatcgc gttccgggcc gcctgcgacc tcgtgttcaa gggctgcgcg caacccaacg
     1681 gctacaccga accgctgctg cacgcgcacc ggcgcgcggc caaggcccgc gcgtaggtcc
     1741 ggatttatcc ggacaatgtc cggctgaagc cggacctacg acgggatatc gccggcgcgg
     1801 atcgccatgt cgtaggtgaa cgcactgtcg tcgtcctcgc gcgggaagcg cgcgcggcgg
     1861 cggtacggga aggtgtcgaa gacatagccg gcgcgtatcg ccgcctgcag cttctgccag
     1921 tagcggtagt cgaagagatc cgcgtgctgg gcctcgaagg ccgccttggc gtgggggttg
     1981 ccggagaaga acaggcggaa ctcctccggg aacacgtcgt tctcggccac cgtgtaccag
     2041 ggcgtgccgg ccatttcctc ggtctcgtcg cgcggctcgg gaatcctgcg gaaattgcac
     2101 tccgtgagcg ggcagatctc gtcgtagtcg tagaacacta cgcgaccgtg gcgcgtgacg
     2161 ccgaagttct tgagcagcat gtcgccgggg aatatgttcg ccgccgccat ctgcttgatc
     2221 gcgttgccgt actcgtcca
//
LOCUS       DAORUQ010000335         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_75395_length_2230_cov_8.215172, whole genome shotgun sequence.
ACCESSION   DAORUQ010000335 DAORUQ010000000
VERSION     DAORUQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75395_length_2230_cov_8.215172"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLW13_16260"
     CDS             complement(<1..1043)
                     /locus_tag="PLW13_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY71985.1"
                     /translation="MIGPNLSEWALSKQSLVVFLMIVAVVAGGLSFTQLGRGEDPVFT
                     FRTMVVGAAWPGATVDETLLQVTERLERTLQETDHLDRIRSYTTAGQTTIFVDLKQST
                     PPAAVPDVWYQVRKNIGDMRQSLPSGVQGPVFNDDFGSTFGIIYAFTADGFTFRELRD
                     YVESARSRLLQVPDVSEIEVLGAQDEQIYIEFSTEKLAGLRLDLPGIAAALQAQNAVR
                     PAGVMQTNQERVFLRVSGSFDTERDVEDLTIVAGDRILRLGDIATVRRGQVDPPQPMF
                     RVNGEPAIGLAIAMRDAGDILALGENVRAEMADIKSALPVGIEPVLVADQAVTVDVAI
                     NDFMTSLWQAIII"
     gene            complement(1040..2161)
                     /locus_tag="PLW13_16265"
     CDS             complement(1040..2161)
                     /locus_tag="PLW13_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY71986.1"
                     /translation="MSVIHAARCSVNLPPMRAIIACAVLSLLAGCSGDDAEQATEIRP
                     VRVVTVADRASSGAVSLTGTVQAQTEINLSFRIDGRMIERTVDVGDRVEPGQLVARLD
                     PQNEESALQSVRAQLTAARAQLVEAQSNHARMRDLVAEDAVSRATFDQAVAMLETAQA
                     QVESMRSQVTLAENRLSYTRLVADVAGVVTARGPEPGEVVPAGRMVVQVAREGSRDAV
                     FGVPAAIKDSAKAKSNPEITVTLVSDPSVKATGRIREISPRADPVTGTFAVRVGLVDP
                     PPSMRLGSTVNGRLGFDATVGIEIPATALVRSEGKVAVWVVDPQAQTVSLRTIMVQAQ
                     DPANVRVTSGLDNGDSVVTAGVHALRPGQKVRPLEGANP"
BASE COUNT          368 a          779 c          716 g          367 t
ORIGIN      
        1 atgatgatga tcgcctgcca cagcgaggtc atgaaatcgt tgatcgccac atccacggtc
       61 accgcctggt cggccaccag taccggctcg ataccgacgg gcagcgcgga cttgatatcc
      121 gccatctcgg cgcgcacgtt ctcgcccagt gcaaggatgt cgccggcgtc acgcatcgcg
      181 atggcgagac cgatggcggg ctcgccgttg acgcggaaca tcggctgcgg cggatcgacc
      241 tggccgcggc gcaccgtggc gatatccccg agacgcagga tgcgatcgcc cgcgacgatg
      301 gtcaggtcct cgacatcgcg ctcggtgtcg aatgaaccgc tgacacgcag gaacacgcgc
      361 tcctggttcg tctgcatcac accggcggga cgtaccgcgt tctgcgcctg cagggcggcc
      421 gcgatacccg gcagatcgag gcgcaggccg gccagctttt cggtcgagaa ctcgatgtag
      481 atctgctcgt cctgcgcgcc caggacttcg atttccgaaa cgtcaggcac ctgcaacagg
      541 cgtgagcgcg ccgactcgac gtagtcgcgc agttcgcgaa aggtgaatcc atcggcggtg
      601 aaggcataga tgatgccgaa ggtgctgccg aagtcgtcgt tgaataccgg cccctggacg
      661 ccggacggca ggctctggcg catgtcgccg atattcttgc gcacctggta ccagacgtcg
      721 ggcaccgcgg caggcggcgt ggattgcttg agatccacga agatggtggt ctgacccgcc
      781 gtggtgtaac tgcgaatccg gtcgaggtga tccgtctcct gcagcgttcg ctcgagccgc
      841 tcggtcacct gcagcagggt ttcgtccacc gtcgcgcccg gccaggccgc gccgaccacc
      901 atggtgcgga acgtgaagac aggatcctcg ccgcgcccca actgcgtaaa cgacagcccg
      961 ccggcaacga ccgcgacgat catcaggaat acgacgaggg actgcttgga gagcgcccac
     1021 tcggagaggt tcgggccgat cacgggttag cgccctccag cgggcggacc ttctgcccgg
     1081 gacgcagcgc atgcaccccc gccgtcacca cggagtcgcc attgtccagc ccggaggtga
     1141 cccgcacatt cgccggatcc tgcgcctgca ccatgatcgt gcgcagcgag acggtctgcg
     1201 cctgcggatc cacgacccag accgcaacct tgccctccga gcgtaccagc gccgtggcgg
     1261 gtatctcgat gccgacggtc gcatcgaagc cgaggcgccc gttcaccgtg ctgcccaggc
     1321 gcatcgacgg gggcggatcg accagcccca ccctgacggc aaacgtgccg gtcacgggat
     1381 cggcgcgggg agagatttca cgaatcctgc cggttgcctt gacgctcggg tcgctgacca
     1441 gggtcacggt gatctccggg ttggacttgg ccttcgcgct gtccttgatc gccgccggca
     1501 cgccgaacac cgcgtcgcgg ctgccttcgc gcgccacctg cacgaccatg cgccccgctg
     1561 gcacgacttc accgggctcc ggtccgcgag cagtgaccac gcccgccaca tcggctacca
     1621 ggcgcgtata gctcaggcgg ttctccgcca gcgtcacctg cgaacgcatg gactccacct
     1681 gcgcctgggc cgtctcgagc atggccaccg cctggtcgaa cgtcgcgcgc gagaccgcgt
     1741 cctcggcgac caggtcgcgc atgcgcgcgt gattgctctg cgcctccacg agttgcgccc
     1801 gcgccgcggt caactgcgcg cgcaccgact gcagggcgct ctcctcgttc tggggatcga
     1861 ggcgcgccac cagctgaccg ggctcgaccc tgtcgccgac atcgaccgtg cgctcgatca
     1921 tgcgtccgtc gatccggaat gacaggttga tttccgtctg cgcctgcacc gttccggtca
     1981 gtgaaacggc gccgctgctg gcgcggtccg caaccgtaac gacacgcacc gggcggattt
     2041 cggttgcctg ctcggcatcg tcgcccgaac agcccgccaa cagcgacagg acggcacacg
     2101 cgatgatcgc gcgcattggg ggcaggttga cactacacct ggcggcgtgg atcacggaca
     2161 cgttatctcc ttactggcca ttgtgtagct gttgttgcca tcgagcccgt gaacaccgcg
     2221 ctgcgcggct
//
LOCUS       DAORUQ010000336         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_75510_length_2228_cov_5.481362, whole genome shotgun sequence.
ACCESSION   DAORUQ010000336 DAORUQ010000000
VERSION     DAORUQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75510_length_2228_cov_5.481362"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLW13_16270"
     CDS             <1..289
                     /locus_tag="PLW13_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HQY71987.1"
                     /translation="GWEGFTDHVVDVLNRERESLVFLLWGSYAQAKGKVIDARRHRVL
                     KAPHPSPLSAHRGFLGCGHFSATNQWLTRGGHAPVDWALPPRAALELPGAS"
     gene            362..>2228
                     /locus_tag="PLW13_16275"
     CDS             362..>2228
                     /locus_tag="PLW13_16275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQY71988.1"
                     /translation="MDNPLLDFSGLPRFDAIRPEHIAPAIEILLAEAEAAVAHAETIA
                     PVTWSGFVTPLEDATERLWRAWGQVVHLQAVADTPELREAYNANLPKVTRFGAALGQN
                     LALFAQYRVLAAAPEYAGYDASRRKVVEHALRDFRLGGAELGDADKARFAAIQEELSA
                     LSATFSQNVLDATDAFSLHVDNEARLTGLPAEVVAAARSAAEKDGAPGWKLTLQMPCY
                     LPVQAYADDRALREALYRASGLRASESGPAALDNSGNIDKILALRAELASLLGFASYA
                     DYSLATKMAQSPAEVLGFLRDLAARALPHARRDRTELELFARDELGIDDLQPWDVAYA
                     SDKLRQARYSYSGQEVKRYFTEPRVLAGLFEVIESLYGVRVREDSAPAWHADVRFFRV
                     EDRDGRLLGQFYLDLYAREGKRGGAWMDDARNRRVLPASTQTPLVYLVCNFGKGMDGK
                     PATFSHDEATTLFHEMGHGLHQLLTAIGELPVAGINGVEWDAVELPSQFMENFCWEWA
                     RVQAMTAHVDTGEPLPRELFDKMLAARNFQSGMATVRQLEFALFDMALHGDFDPAQDS
                     VLALLGRIRDEVAVNRPPAWHRFPHQFSHIFAGGYAAGYYSYKWAEVLSADAYAAF"
BASE COUNT          346 a          783 c          743 g          356 t
ORIGIN      
        1 gggctgggaa ggcttcaccg accacgtggt cgacgtgctc aaccgcgagc gcgaaagcct
       61 ggtgttcctg ctctggggca gctacgcgca ggccaaggga aaggtcatcg acgcgcgccg
      121 ccaccgcgtg ctgaaggcgc cgcatccctc gccgctgtcc gcccatcgcg gtttcctggg
      181 ctgcgggcat ttctccgcga ccaaccagtg gctgacgcgt ggcgggcacg cgccggtgga
      241 ctgggccctg ccgccgcgtg ctgcgctgga gctaccggga gcatcttgat gtgcatgcgc
      301 ggttgatttg ctgcgcttct gcacgcatct cgattccatt gcattccttt cgcaggtaca
      361 catggacaac ccgctactcg acttctccgg cctgccgcgc ttcgacgcga tccgccccga
      421 acacatcgcg ccggcgatcg agatattgct cgccgaggcc gaagcagcgg tcgcccacgc
      481 cgagaccatc gcgccggtga cctggtccgg cttcgtgacc ccgctggagg acgccaccga
      541 gcgcctgtgg cgcgcctggg gccaggtcgt ccacctgcag gcggtggccg acaccccgga
      601 actacgcgag gcctacaacg ccaacctgcc gaaggtgacc cgtttcggcg cggcgctggg
      661 ccagaacctc gcgctgttcg cgcagtaccg cgtgctggcc gctgcgccgg aatacgcagg
      721 ctacgacgcc agccgccgca aggtggtcga acacgcgctg cgcgatttcc gcctcggggg
      781 cgccgaactg ggcgatgccg acaaggcgcg cttcgccgcg atccaggaag agctgtcggc
      841 gttgtcggcg acgttctcgc agaacgtgct ggatgccacc gacgccttca gcctgcatgt
      901 ggacaacgaa gcgcggctca ccggcctgcc cgccgaggtc gtggccgccg ctcgctccgc
      961 cgccgagaag gatggagcac cgggctggaa gctcaccctg cagatgccct gttacctgcc
     1021 ggtgcaggcc tatgccgacg accgcgcgct gcgcgaggcg ctctatcgcg ccagcggcct
     1081 gcgcgcgtcg gaaagcggcc cggccgcgct cgacaacagc gggaacatcg acaagatcct
     1141 cgccttgcgc gccgagcttg cctcgctgct cggcttcgcc agctacgccg actactcgct
     1201 ggcgaccaag atggcgcagt cgccggcgga ggtgctcggg ttcctgcgcg atctcgccgc
     1261 gcgtgcgttg ccgcatgcgc gacgcgatcg caccgagctg gagttgttcg cccgcgatga
     1321 gctcggcatc gatgacctgc agccctggga cgtcgcctat gccagcgaca agctcaggca
     1381 ggcgcgctac agctattccg ggcaggaggt gaagcgctat ttcaccgagc cgcgcgtgct
     1441 cgccggcctg ttcgaggtga tcgagtcgct gtacggcgtg cgcgtgcgcg aggacagcgc
     1501 gccagcctgg catgcggacg tgcggttctt ccgggtcgag gaccgcgacg gtcgcctgct
     1561 cggccagttc tacctcgatc tgtacgcccg cgagggcaag cgcggcggcg cctggatgga
     1621 cgacgcccgc aaccggcgtg tcctccccgc cagcacgcag acgcccctcg tctacctggt
     1681 gtgcaatttc ggcaagggca tggacggcaa gccggcgacc ttcagccatg acgaagccac
     1741 caccctgttc cacgagatgg gacacggcct gcaccagttg ctgaccgcca tcggcgagct
     1801 gccggtcgcc ggcatcaacg gcgtggaatg ggatgcggtg gaactgccca gccagttcat
     1861 ggagaacttc tgctgggaat gggcgcgggt gcaggcaatg accgcgcacg tggacaccgg
     1921 cgagccgctg ccgcgcgaac tcttcgacaa gatgctggca gcgcggaatt tccagagcgg
     1981 catggccacc gtccgccagc tggagttcgc cctgttcgac atggccctgc acggcgattt
     2041 cgatccggcg caggacagcg tgctcgccct gctgggacga atccgcgacg aggtggcggt
     2101 caatcgccca cccgcctggc accgtttccc gcaccagttc agccacatct tcgccggtgg
     2161 ctatgcggcc ggttactaca gctacaaatg ggccgaggtg ctcagcgccg acgcctacgc
     2221 cgccttcg
//
LOCUS       DAORUQ010000337         2219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_76014_length_2219_cov_9.052218, whole genome shotgun sequence.
ACCESSION   DAORUQ010000337 DAORUQ010000000
VERSION     DAORUQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76014_length_2219_cov_9.052218"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLW13_16280"
     CDS             complement(<1..392)
                     /locus_tag="PLW13_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HQY71989.1"
                     /translation="MSQFAFLQREWTAVFDAAARAEAAVHADPRTSCFYARRALELAV
                     GWAYKHDASLRLPYQDNLSALIHDPSFKEAAGEAVFSKARVINTLGNRAVHGHRAIPL
                     DDAMVAVRELFHVAYWLARTYGRAARPAP"
     gene            complement(389..619)
                     /locus_tag="PLW13_16285"
     CDS             complement(389..619)
                     /locus_tag="PLW13_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71990.1"
                     /translation="MKRDPRGSTGQRATPASKWLIHKAAGAGELQRAGRWLQFRSNAL
                     QTAHRVKAFQPFTWIAGAAMLKAGYHKRGIEG"
     gene            complement(616..>2219)
                     /locus_tag="PLW13_16290"
     CDS             complement(616..>2219)
                     /locus_tag="PLW13_16290"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY71991.1"
                     /translation="PEGIHLEFKEAKQNYHFDKLVEYCVALANEGGGKIILGVTDRRP
                     RRIVGTAAFAEPGRTEAGLHDRLSHRIPVEEVQTADARVLVVHVPARLPGTAWQIDGR
                     YLKRAGDELTGLSDAELKAMFAETGPDFSAEVCPGAGIGDLSPEAIAAFRGRWATKTR
                     DERKTRWTDEQTLANAELLLEGRITYAALILFGTRAALGRWLAQAELVFEYRSSEASG
                     PAADREEYREGFFLWHDGIWNKINLRNDRQSYQDGLFRVELPTFDEVSVREALLNAVA
                     HRDYRQGGSVFVRQFAQRLEVVSPGGLPMGITPANILDQQNPRNRRLAEALAKCGLIE
                     RSGQGMNLMFESAIRQGKALPSFAGTSPHEVRLTLYGTVASPAFVRFIERLGEEKLKS
                     FSTYDFLALDCVRREQAMPEHLKACLPGLIAAGAVESIGRGRGTRYILARGLYAALGG
                     KGVYTRKKGLDRGTNKALLLKHIQDNAASGAQMEEFRQVLPALSRSQIQVLLRELRRE
                     ESIHSVGVTKAARWYPGAGPGELQP"
BASE COUNT          344 a          803 c          687 g          385 t
ORIGIN      
        1 ggcgccggtc gcgccgcgcg accgtaggtg cgggcgagcc agtacgccac gtggaacagc
       61 tcgcgcaccg cgaccatcgc gtcgtcgagc gggatcgcgc gatgcccgtg cacggcgcgg
      121 ttgcccagcg tgttgatgac ccgcgccttg ctgaacaccg cctcgcctgc ggcttccttg
      181 aaactggggt cgtggatcag cgccgacagg ttgtcctggt acggcagccg cagcgaggca
      241 tcgtgcttgt aggcccagcc caccgcgagt tctagcgcgc gccgcgcgta gaagcacgac
      301 gtgcgtggat cggcgtgcac agccgcctcg gccctggccg ccgcgtcgaa gacagcggtc
      361 cactcgcgct ggaggaaggc gaactgactc acccctcgat gcccctcttg tgatagccag
      421 ccttcagcat ggcggccccg gcgatccatg tgaaaggctg gaacgccttc actcggtggg
      481 ccgtttgcaa cgcattgctc cggaattgca accagcgtcc agctcgttgc aattcgcccg
      541 ctccagcagc cttatggatc agccacttag aagcgggcgt ggcccgctgg ccggtcgaac
      601 cccgcggatc gcgcttcacg gttgcaattc tccagggccc gctccgggat accagcgggc
      661 ggcctttgtg accccgacgc tgtgtatcga ctcttcccgg cgaagttccc gcagcagtac
      721 ctgaatctgg cttcgcgaca gcgccggcag cacttgccgg aactcctcca tctgcgcgcc
      781 gctcgccgcg ttgtcctgga tgtgcttcag cagcagcgcc ttgttcgtcc ctcgatccag
      841 ccccttctta cgggtgtaga cgcccttgcc gcccaacgcg gcgtaaagcc cccgcgccag
      901 gatgtaccgg gtcccccgac cgcgtccgat cgattccacc gcgccggcgg cgatcagacc
      961 cggcagacaa gccttcagat gctcgggcat cgcttgctcg cgccgcacgc aatcgagcgc
     1021 aaggaagtcg taggtcgaga acgacttgag cttctcctca cccagccgct cgatgaaacg
     1081 cacgaatgcc gggctggcga ccgttccata cagggtcagc cgcacctcgt gcggcgacgt
     1141 gcccgcgaag ctgggcagcg ccttgccctg acggatcgcg ctttcgaaca tcaggttcat
     1201 gccctggccc gagcgctcga tcagcccgca cttcgccagc gcctcggcca gccgccgatt
     1261 gcgcgggttc tgctggtcga ggatgttcgc gggggtgatc cccatcggca ggccgccggg
     1321 acttaccacc tcgagccgct gggcgaattg ccgcacgaac accgaaccgc cctgccgata
     1381 gtcccgatgc gctacggcat tgagcagcgc ttcgcgcacc gacacttcgt cgaaggtcgg
     1441 cagttcgacg cggaacaacc cgtcctgata gctctgcctg tcgttgcgca gattgatctt
     1501 gttccagatc ccgtcgtgcc agaggaagaa gccctcccgg tactcctcgc ggtcggccgc
     1561 agggcccgac gcctcggagg accggtactc gaacacgagt tccgcctgcg ccagccagcg
     1621 ccccagcgcc gcgcgcgtgc cgaacaggat cagtgccgcg taggtgatgc gcccctccag
     1681 cagcagctcg gcgttggcga gcgtctgctc gtcggtccat cgcgtcttgc gctcgtcgcg
     1741 cgtcttggtg gcccaccggc cccgaaatgc ggcgatcgct tccggcgaca ggtcgccgat
     1801 gcccgcgccg gggcagacct ccgccgagaa atcgggcccc gtctcggcga acatggcctt
     1861 cagttccgca tcgctcagcc cggtcagctc gtcacccgcc cgcttgagat accgcccgtc
     1921 gatctgccag gccgtgcccg gaagtcgcgc cggtacatgg acgaccagca cgcgagcatc
     1981 cgctgtttgc acctcttcta ccggaatgcg atgcgagagc cggtcgtgca gacccgcttc
     2041 cgtgcgcccc ggctcggcga acgctgccgt gccgacgatt cggcgcggcc gccggtcggt
     2101 cacgccgaga atgatcttgc caccgccttc gttcgccaag gccacgcagt actccaccag
     2161 cttgtcgaag tgatagttct gcttcgcctc cttgaactcg aggtggatgc cttcaggct
//
LOCUS       DAORUQ010000338         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_78120_length_2182_cov_8.989657, whole genome shotgun sequence.
ACCESSION   DAORUQ010000338 DAORUQ010000000
VERSION     DAORUQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78120_length_2182_cov_8.989657"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLW13_16295"
     CDS             <1..736
                     /locus_tag="PLW13_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQY71992.1"
                     /translation="TLAEVLPAADYARWTPLKARYIGRSRAVEKRRPLFAAWKLYEEA
                     LDSVGLSVRVPVAKVVRRAAKRHDVSLTQPEVRIVLDKPGATLKDFARSPLDDLECFR
                     RTLSHVESDLGTLQVRANAWATGDLELLRSVPFTDATRACLDAVFEAGFAERSGMDNI
                     EEQLRAQWLQAVEAALARHPTSFGMLPISLLLREDGWLADLRERGYAVETPWEREARE
                     LEADDELLPALDAEPRPPAAEPPRRQ"
     gene            complement(798..1868)
                     /locus_tag="PLW13_16300"
     CDS             complement(798..1868)
                     /locus_tag="PLW13_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71993.1"
                     /translation="MDRQRAFAPVRSQDAASTSHRARRGPARVLHGLVAFCSILMPIS
                     VVAQAPACPPPVPGRLFDSGFELPGGGDLAAPGPYTAATVSGTSSQGGRTTPWVAHYP
                     VGSSGRPLVLFAPGFQIPSAGYSDWATHLATWGFIAVRADPVGGLPPNHVAMSLDLRD
                     VLDDLLAPAALPVSVDVSRVALSGHSLGGKLALMAASGDARVRTLFVLDPINGGGPGG
                     YTTEQPNIVPQPMGSLAVPIGVLGELLDSTSSSGGQACAPTAVNYQTLFQAATASPRA
                     YEWTLAGASHIDFVTNQDQCGFACTFCNPRTLPLAQTHAFMRASAVAFLRNSLQGESG
                     LCPWLTGALLPGAVGVRQAPPP"
BASE COUNT          337 a          749 c          789 g          307 t
ORIGIN      
        1 gaccctggcc gaggtgctgc ccgcggccga ctacgcgcgc tggaccccgc tcaaggcccg
       61 ctacatcggc cgcagccgcg cggtggagaa gcgccgcccg ctgttcgcgg cctggaagct
      121 ctacgaggaa gcgctcgaca gcgtggggct gagcgtgcgc gttccggtgg ccaaggtcgt
      181 gcggcgcgcg gcaaagcgac acgacgtgtc gctgacgcag ccggaggtcc gcatcgtgct
      241 ggacaagccc ggcgccacgc tcaaggattt cgcccgttcg ccgctcgacg acctcgagtg
      301 cttccggcgc acgctgtcgc acgtggagtc cgacctcggc accttgcagg tgcgcgccaa
      361 cgcctgggct accggcgacc tcgagctgtt gcgcagcgtg cccttcaccg atgccacgcg
      421 cgcctgcctc gacgccgtgt tcgaggcggg cttcgccgag cgcagcggca tggacaacat
      481 cgaggaacaa ctgcgcgcgc agtggctgca ggcagtggag gcggcactgg cgcgtcatcc
      541 caccagcttc ggcatgctcc cgatctccct gctgctgcgc gaggacgggt ggctcgcgga
      601 tctgcgcgag cgcggctatg cggtcgagac gccgtgggag cgggaggcgc gcgagctcga
      661 ggccgacgac gaactccttc cggccctcga cgcggaaccg cgcccgccgg ccgctgagcc
      721 gccacggcgt cagtgacgcg tctggccggt gagtgctggc ccgacgagtg tccctgacag
      781 cgttctcgcg gtgcggatca gggtggcgga gcctgccgca cgcccaccgc accgggcagc
      841 aaagcgcccg tcagccacgg gcacagcccg gactcgccct gcagggagtt gcgcaggaag
      901 gccaccgcgc tggcgcgcat gaaggcgtgg gtctgcgcca gcggcagcgt tcgcggattg
      961 cagaacgtgc aggcgaaacc acattggtcc tggttggtga cgaagtcgat gtgcgaggcg
     1021 ccggcgagcg tccattcgta cgcccggggc gatgcggtcg ccgcctggaa gagggtctgg
     1081 tagttcaccg cggttggcgc gcaggcttgt cccccgctgc tgctggtgct gtcgagcaac
     1141 tcgccgagta cgccgatcgg caccgcgaga ctccccatgg gctgcggcac gatgttgggc
     1201 tgttcggtcg tatagccgcc tggtccgccg ccattgatcg gatcgaggac gaacagcgtc
     1261 cggacccgcg catcgcccga cgccgccatc aaggcgagct ttccaccgag gctgtgcccc
     1321 gacagggcga cgcgggacac gtccacgctc accggcaagg ccgccggcgc cagcaggtcg
     1381 tcgagcacgt cgcgcaggtc gagcgacatg gcgacgtgat tgggcgggag tccgccgacc
     1441 ggatcggcgc gcaccgcgat gaaaccccag gtagccagat gcgtggccca gtcgctgtag
     1501 ccagccgacg ggatctggaa gccgggcgcg aacagcacca gcggtcgccc gctcgaaccg
     1561 accggatagt gcgccaccca aggcgtagtg cggccgccct gcgaactggt tcccgacacg
     1621 gtggcggcgg tgtacggccc tggcgcggcc agatcgccgc ctccaggcaa ctcgaagccg
     1681 ctgtcgaaga ggcgtccggg taccggtggc gggcacgccg gtgcctgggc gacgacgctg
     1741 atcggcatga ggatcgagca gaaggcaacc aggccgtgga gaacgcgggc cggacccctg
     1801 cgcgcgcggt gcgacgtgct cgccgcatcc tgcgacctca ccggggcaaa cgcacgttgt
     1861 cgatccatga ggcaaagacc tcgcgacggt agtccgacac tgatgcggtg cctacggtac
     1921 ctcaacgggc ccttgtcgat gcgacggccg gcctggcgcg aagttcgtgc agtcgcgatc
     1981 ggcccggaaa gcgcgggatt gctgcccgac gctcagaaca ccgcgtggtc gccgcgctcg
     2041 gccgccttgg tgccgcacac aagttcggca tggtagtcga acagggtttc gctgccggtg
     2101 gagggcgtcg cgtcggcgtc gtagcgcccg gtcgcggggt ccagcaccag catcgattcg
     2161 gtcgcgtaat agcgcccgat gc
//
LOCUS       DAORUQ010000339         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_79798_length_2155_cov_5.591429, whole genome shotgun sequence.
ACCESSION   DAORUQ010000339 DAORUQ010000000
VERSION     DAORUQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79798_length_2155_cov_5.591429"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLW13_16305"
     CDS             complement(<1..376)
                     /locus_tag="PLW13_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HQY71994.1"
                     /translation="MKQAPCSRWQDHTPRSLAALADAGAVAVLPLAAIEQHGEHLPLS
                     TDLDIALGLVAEATGQLRPGVLACVLPPLAIGMSREHTHFRGTLSLSAQTALAALTEI
                     GEGVARAGLRRLVILNSHGGNRA"
     gene            463..2121
                     /locus_tag="PLW13_16310"
     CDS             463..2121
                     /locus_tag="PLW13_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71995.1"
                     /translation="MSLRRLPVFVGLGVSLLALNTLFGFVNHWPGMGVVPDSRVSAEL
                     ALLVLVLALWVRRRAGLSPRATAWLAACACFGVLVRYADVTSRALLGRPVNLYWDGQH
                     AWQVLRMAGAEQNWDKLGGALLGSVVVIGLLFLLLRKLFSLLGQGLGHARVTRPAAVA
                     GLALSLLWLLAPQLGAHVQRIFAEPITGTVVQQVRTLARSLSPGRAEALLPASPAFDA
                     DLSALQGADVLVLFVEAYGAITLDAARIAHPLEPARETFARAIAGSGRAVVSARVVSP
                     TFGGASWLAHAALLSGIDTREPDTYAALLGSRRPSLVGHFAANGYRTVAWVPGIQRPW
                     PEGRFWGFDRYGELDTLGYRGRAFGYWQVPDQAAMALLERDELSADARIARPGPRFIV
                     FPTVSTHAPFVPIAPFRSDWAALAGTEAYSEAELARALATPEDWTHPTPHYVDSLAYT
                     FEWLSSFVAERADPELVLIVIGDHQPIGAVTGPGAPWDVPVHVIGRNAELQARLRAQG
                     FVDGLRPSAQPLGPMHELTQLLVRSFGDVGATVPPDVRLEPGPR"
BASE COUNT          265 a          761 c          785 g          344 t
ORIGIN      
        1 ccgcgcggtt gccaccgtga ctgttgagga tcaccaggcg ccgcaaaccg gcgcgggcga
       61 caccctcgcc gatctccgtg agcgcggcga gggcggtctg cgccgaaagg ctgagcgtgc
      121 cgcggaaatg cgtgtgttcc cggctcatgc cgatcgcgag cggcggcagc acgcaggcga
      181 gcacaccggg ccgcaactgc ccggtcgcct cggcgacgag accgagcgcg atgtcgagat
      241 ccgtggagag cggcaggtgc tcgccgtgct gctcgatcgc cgcgagcggc agcaccgcga
      301 ccgcgccggc gtcggcgagc gcggcgaggc tgcgcggcgt atggtcctgc cagcgcgaac
      361 aaggggcttg cttcacggga tcgtggctcc tggggtgccc gtgcgcagcc tgcctgcgcc
      421 cgtcacgcgc cgtcatgcta aacaaacgcg gcggccgggg cgatgagttt gcgcaggctg
      481 ccggtcttcg tcgggctcgg cgtgtcgctg ctcgcgctga acaccctgtt cggcttcgtg
      541 aaccactggc cagggatggg cgtggtcccg gattcccggg tatcggcgga actggcgctg
      601 ctggtgctgg tgctcgcact gtgggtgcgc cggcgagccg gcctctcccc gcgcgccacg
      661 gcgtggctcg ccgcgtgcgc ctgcttcggc gtgctcgtgc gctacgccga cgtcaccagc
      721 cgcgcgctgc tcgggcgccc ggtgaatctc tactgggacg gacaacacgc ctggcaggtg
      781 ctgcgcatgg ccggcgcgga gcagaactgg gacaagctcg gcggggcgct gctcggcagc
      841 gtcgtggtga tcgggctgct gttcctgctg ctgcgcaaac tgttctcgct gctcggacag
      901 ggcctgggcc acgcccgggt cactcgcccc gcggcggtcg cggggctcgc actgtcgctg
      961 ctgtggctgc tcgccccgca actcggggcg cacgtgcagc ggatcttcgc cgagccgata
     1021 acgggtaccg tggtgcagca ggtccgcacg ctggcgagat cgctgtcgcc agggcgcgcg
     1081 gaggcactgc tgccggcgag ccccgccttc gacgccgatc tctcggcgtt gcagggtgcc
     1141 gatgtgctgg tactgttcgt cgaggcctac ggcgcgatca cgctggacgc cgcgcgcatt
     1201 gcccaccctc tggagccggc ccgcgagacc ttcgcccggg cgatcgccgg gagcggtcgc
     1261 gcagtcgttt cggcgcgggt ggtttcgccg acgttcggcg gcgcctcgtg gctcgcgcac
     1321 gccgccttgc tctccggcat cgacacccgc gagcccgaca cctatgcggc gctgctcggt
     1381 agccgccgcc cctcgctcgt cggtcacttc gccgccaacg gctatcgaac cgtcgcctgg
     1441 gtgccgggca tccagcgtcc gtggccggaa ggtcgcttct ggggctttga tcgctacggg
     1501 gaactggaca cgctcggcta ccggggccgc gccttcggct actggcaggt gccggaccag
     1561 gccgcgatgg cgttgctgga gcgcgacgag ctttccgccg acgcgcggat cgcgcgtccc
     1621 gggccgcgct tcatcgtgtt tcccacggtc agcacccacg cgcccttcgt gccgatcgcg
     1681 ccgttcagga gcgactgggc ggcgctcgcc gggaccgagg cgtattcgga ggccgagctg
     1741 gcaagggcgc tcgccactcc ggaggactgg actcacccca cgccgcacta cgtcgactcg
     1801 ctcgcctaca ccttcgaatg gctgtcgtca ttcgtcgcgg agcgtgccga ccccgaactg
     1861 gtcctgatcg tgatcgggga tcatcagccg atcggcgcgg tcaccgggcc gggagcgccg
     1921 tgggacgtgc cggtacacgt catcggccgc aacgcggaac tccaggcgcg actgcgggcg
     1981 cagggtttcg tcgacggact gcgaccttcc gcgcagccgc tcggcccgat gcacgaactg
     2041 acgcagttgc tggtgcgcag tttcggcgac gtcggggcga ccgtcccacc ggatgtccgg
     2101 ctcgagccgg gcccacgatg acggcgcttg cgattcgagg cgcgcggacc gtagc
//
LOCUS       DAORUQ010000340         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_80376_length_2146_cov_6.803922, whole genome shotgun sequence.
ACCESSION   DAORUQ010000340 DAORUQ010000000
VERSION     DAORUQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80376_length_2146_cov_6.803922"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="secA"
                     /locus_tag="PLW13_16315"
     CDS             complement(<1..574)
                     /gene="secA"
                     /locus_tag="PLW13_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253093.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQY71996.1"
                     /translation="MVLSLIRKLVGTKNSRELKRMRRVVERINALEPQFEALDDAGLR
                     AMTAKFRERLAAGQTLDQLLPEAFAAVREAGRRALGMRHFDVQLIGGITLHEGRIAEM
                     RTGEGKTLVATLPVYLNALSGKGVHVVTVNDYLARRDANWMAPIYQFLGMSVGVILPN
                     QDLESKRAGYAADITYGTNNEFGFDYLRDNM"
     gene            complement(763..1692)
                     /locus_tag="PLW13_16320"
     CDS             complement(763..1692)
                     /locus_tag="PLW13_16320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY71997.1"
                     /translation="MRVILLQDFPASSRTLALPVRMVWALALLCVVCLGAAGFGGYRL
                     ALAQAGELPADLVTAWGAELSALAVRTGELHESTEREGRAYAARIATLQARLLRMEAV
                     GERLAVAAQLDKGEFDFDQEPALGGPFSPEPASLGGSELGETLRQLASQIKDRERQLD
                     VMEKLLVNRKLAGDISLAGLPVADGYISSRFGPRADPFDGRRAMHKGVDFTASAGSDV
                     FAIASGVVVWAGYEKDYGNLVEIRHSNGYRTRYAHNREYLVKPGDIVQKGQVIARVGQ
                     TGRASGAHLHLEVLNGDRQIDPMHYISTVRGDA"
     gene            complement(1886..>2146)
                     /locus_tag="PLW13_16325"
     CDS             complement(1886..>2146)
                     /locus_tag="PLW13_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716977.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQY71998.1"
                     /translation="MNGEIKVSVVATGLGEERKAPVKVIDNTVGGGLASGQPNYRDLD
                     KPAVGRRQAGTREIAERPPMAVGAERDLDYLDIPAFLRRQAD"
BASE COUNT          331 a          761 c          713 g          341 t
ORIGIN      
        1 ccatgttgtc gcgcaggtag tcgaaaccga attcgttgtt ggtgccgtag gtgatgtcgg
       61 cggcgtaacc cgcgcgcttg ctctcgaggt cctggttcgg aaggatcacg cccacgctca
      121 tgccgaggaa ctggtagatg ggcgccatcc agttcgcgtc gcgccgcgcc aggtagtcgt
      181 tcacggtcac cacgtgcacg cccttgcccg acagcgcatt caggtacacc ggcagcgtgg
      241 cgaccagcgt cttgccctcg ccggtgcgca tctcggcgat acgcccctcg tgcagcgtga
      301 taccgccgat cagctgcacg tcgaagtggc gcatcccgag agcccggcga ccggcctcgc
      361 gcactgccgc gaaggcctcg ggcagcaact ggtccagggt ttgaccggcc gcgaggcgtt
      421 cacggaactt cgcggtcatc gcgcgcaggc cggcgtcatc cagcgcctcg aactgcggct
      481 cgagcgcatt gatgcgctcg accacgcgac gcatgcgctt cagttcacga ctgttcttgg
      541 taccgacgag tttgcgtatc agcgaaagga ccatggttct tgcagttttc cagcgtggcg
      601 cggcgatgcg gcgtgtatgc ccctgcccgg cggcgccgga tccggaacgt ccggcacggg
      661 ccccgcggct gggcggcgcg aaattatata ccggggcgac acggcgcggg cgcgccgcgg
      721 agaattaccg aagccggccg tcgaagggcc cgcaaggcgg gatcaggcgt cgcccctgac
      781 cgtgctgatg tagtgcatcg gatcgatctg gcgatcgccg ttcagcacct ccaggtgcag
      841 gtgcgcaccg ctggcgcggc ccgtctgccc cacgcgcgcg atcacctgcc ccttctgcac
      901 gatgtcgccg ggcttcacca gatactcgcg gttgtgcgcg tagcgcgtgc gatagccgtt
      961 gctgtggcgg atctcgacga ggttgccgta atccttctcg tagccggccc agaccacgac
     1021 gccggaggcg atggcgaaca cgtccgagcc ggcgctcgcc gtgaaatcca cgcccttgtg
     1081 catggccctg cggccatcga agggatctgc gcgcggcccg aagcgcgagg agatataacc
     1141 gtcggccacc ggcaagcccg cgagcgagat gtccccggcc agcttgcggt tgaccagcag
     1201 tttctccatt acatcgagct gacgctcgcg gtccttgatc tgcgaggcca gctggcgcag
     1261 cgtctcgccc agctcgctgc cgcccaggct cgccggctcc gggctgaacg gcccgcccag
     1321 cgccggctcc tggtcgaaat cgaactcgcc cttgtcgagc tgcgccgcga ccgcgagccg
     1381 ttcgccgacc gcctccatgc gcagcagacg cgcctgcagc gtggcgatgc gcgcggcata
     1441 ggcgcgccct tcgcgctccg tgctctcgtg cagctccccg gtgcgtaccg ccagcgcgga
     1501 cagctcggcc ccccaggccg tgaccaggtc cgcgggcagt tccccggcct gcgccagcgc
     1561 cagccgatag cccccgaaac cggcggcgcc caggcagaca acgcaaagca gcgcaagggc
     1621 ccagaccatg cggacgggaa gcgccagggt tctcgatgag gccgggaagt cctggagcaa
     1681 gatcactctc atgcggcggc accagatcct gattgcttgt tttttgatcg acctggcggc
     1741 cgaaccggat agcagacggg cgcgaatatg ccacacggac agcaaaattg ccaaattgta
     1801 ccgcgcggcg ccccaggcgc gccgcacgcg caggcggccc ccgggccccg tgcgcgacgg
     1861 ggtccggggg ctgcgacggc gatcctcagt ccgcctggcg gcgcaggaac gcggggatgt
     1921 cgagataatc gagatcacgc tccgcgccga ccgccatcgg cggccgctcg gcgatctcgc
     1981 gcgtacccgc ctggcggcgc ccgaccgccg gcttgtcgag gtcgcggtaa ttgggttgcc
     2041 ccgaggccag tccacccccg accgtgttgt cgatcacctt gaccggtgcc ttgcgctcct
     2101 cgccgaggcc ggtggcgacc accgagacct tgatctcgcc gttcat
//
LOCUS       DAORUQ010000341         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_81555_length_2128_cov_5.493970, whole genome shotgun sequence.
ACCESSION   DAORUQ010000341 DAORUQ010000000
VERSION     DAORUQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81555_length_2128_cov_5.493970"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLW13_16330"
     CDS             <1..864
                     /locus_tag="PLW13_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY71999.1"
                     /translation="IVSDNFHPARSGSGSLYTVEHFAAVQQRLAPGGLFCQWLPLHQL
                     DLDTLRSIVRAFLAVYPEGRAMLATNSLDTPVLGLLARRGGGGFDAARLRGRLAGAAM
                     LRSPADFGLEDELALLGSFIAGPRSLARFAGTAPLNTDDHPVVAYRAPRVTYAPDSLP
                     RDRLLALLRELVISPEEVLATPPDAALASRLAAYWRARERFLEAGRHVRPSADVQRML
                     AQVGEPLLEVLRLSPDFRPAYDPLLRMAIALARDDVAAARTLLAQLQAAQPARREAGQ
                     LLRELPGAGLP"
     gene            complement(878..>2128)
                     /locus_tag="PLW13_16335"
     CDS             complement(878..>2128)
                     /locus_tag="PLW13_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY72000.1"
                     /translation="YSLSGTWESAHWNASLYAIRYDLDLWSNFTYELDDPVNGDQFQQ
                     VDERAVYGGELAWRLDGALLAARMHNTLGLQWRHDDIDEVGLYRTVARHEVGVIRSDA
                     VEQSSVALFWQNEIHWSDSLRSVVGARVDRYDFSVDAPDVVNINGVALAGNGGTEDDA
                     IASLKGSLVRALGEHAEIYVSAGQGFHSNDARGTTTRLDPASGEAVDPVDPLVRSLGA
                     EIGARLFEAGKWNASISLWQLELDSELVFVGDAGTTEPSRESERKGAELTAYYHFGGA
                     WTLDLEYAVADAQFTSADPSDAALGDEVPGSIDRVFAAGLSARLGEGWFGSLRLRHFG
                     PRALEENGDVESDSSTVLNLRAGYELERWRVTLDVLNLFDSDDHDIDYFYESQLPGES
                     EPVADLHYHVMEPRTLRLYAGYRF"
BASE COUNT          310 a          797 c          688 g          333 t
ORIGIN      
        1 atcgtttccg acaacttcca cccggcccgc agcggatccg gctcgctcta caccgtggag
       61 catttcgcgg cggtacagca gcggctcgcg ccgggcgggc tgttctgcca gtggctgccg
      121 ctgcaccagc tcgacctcga cacgctgcgc agcatcgtgc gcgcctttct cgccgtctac
      181 ccagagggcc gcgcgatgct ggccaccaac agcctggaca cgcccgtgct gggcctgctc
      241 gcgcgtcgcg gcgggggcgg cttcgatgcg gcgcgcctgc gcgggcgcct cgccggcgcg
      301 gcgatgctgc gcagccccgc cgacttcggt ctcgaggacg agctcgcgct gctcggcagc
      361 ttcatcgccg gtccgcgcag cctcgcccga tttgccggca ccgcgccgct gaacaccgac
      421 gaccacccgg tcgttgccta tcgcgcgccg cgcgttacct acgcgcccga ttcactcccg
      481 cgcgaccggc tgctggcgtt gttgcgcgag ctcgtgatca gcccggagga agtcctcgcc
      541 acaccgcccg atgccgcgct ggcgtcccgg ctcgccgcgt actggcgcgc gcgcgagcgc
      601 ttcctcgagg cgggccgcca cgtccggccg agcgccgatg tgcaacgcat gctggcacag
      661 gtgggcgaac cgctgctgga ggtgctgcgc ctgagcccgg atttccgccc cgcctacgac
      721 ccgctgctgc gcatggccat cgccctggcc cgcgacgacg tcgcggcggc acgcacgctg
      781 ctggcgcaat tgcaggcggc acagccggcg cgccgcgaag ccggccagct gttgcgcgaa
      841 ctgcccggcg ccggcttgcc ctgagcgacc tcgccgctca gaaccggtag cccgcgtaca
      901 ggcgcagcgt gcgcggctcc atcacgtggt agtgcaggtc cgccaccggc tcggactcgc
      961 cgggcagttg cgattcgtag aaataatcga tgtcgtgatc gtcgctgtcg aacaggttca
     1021 gcacgtccag cgtcacgcgc cagcgctcca gctcgtagcc ggcgcgcagg ttgagcaccg
     1081 tgctgctgtc ggattcgacg tcgccattct cctccagcgc acgcggaccg aaatggcgca
     1141 ggcgcaggct gccgaaccag ccctcgccga gccgcgcgct caacccggct gcaaacacgc
     1201 gatcgatgga tcccggcacc tcgtcaccca gcgccgcatc ggagggatcg gccgacgtga
     1261 actgcgcgtc ggcaacggcg tactccaggt ccagcgtcca ggccccgccg aaatggtagt
     1321 aggccgtgag ttccgcgccc ttgcgctcgc tctcgcggct cggctccgtg gtgccggcat
     1381 cgcccacgaa caccagttcg ctgtcgagct ccagctgcca cagcgagatc gaggcattcc
     1441 atttcccggc ctcgaacaga cgggcgccga tctcggcacc cagcgaacgc accagcgggt
     1501 ccaccggatc caccgcctcg ccgctcgcgg gatccagcct cgtggtggtg ccgcgcgcat
     1561 cgttggaatg gaagccctgc cccgccgaga catagatctc ggcatgctcg cccagcgcgc
     1621 gcaccaggct gcccttcagg ctggcgatcg cgtcgtcctc ggtgccgcca ttgccggcca
     1681 gggcaacgcc gttgatgttc actacatccg gtgcatcgac ggagaagtcg taacggtcga
     1741 cgcgcgcgcc gacgaccgag cgcaggctgt cgctccagtg gatctcgttc tgccagaaca
     1801 gcgcgacgct gctctgctcg accgcatcgc tgcggatcac gcccacctcg tgccgcgcca
     1861 cggtgcgata caggccgact tcgtcgatat cgtcatggcg ccactgcagg cccagcgtgt
     1921 tgtgcatgcg cgcagccaat aacgcgccat cgagacgcca ggccagctcg ccgccgtaga
     1981 cggcgcgctc gtcgacctgc tggaactggt cgccattgac cggatcatcg agctcatagg
     2041 tgaagttcga ccacaggtcc aggtcgtagc ggatcgcgta gagcgaggcg ttccagtggg
     2101 cgctctccca ggtcccgctc aggctgta
//
LOCUS       DAORUQ010000342         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_81788_length_2124_cov_6.388110, whole genome shotgun sequence.
ACCESSION   DAORUQ010000342 DAORUQ010000000
VERSION     DAORUQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81788_length_2124_cov_6.388110"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /gene="glcF"
                     /locus_tag="PLW13_16340"
     CDS             complement(<1..140)
                     /gene="glcF"
                     /locus_tag="PLW13_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017683328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HQY72001.1"
                     /translation="MDAPLADFIRDTPEGEAARAILRKCVHCGFCTATCPTYQLLGDE
                     LDG"
     gene            complement(158..1228)
                     /gene="glcE"
                     /locus_tag="PLW13_16345"
     CDS             complement(158..1228)
                     /gene="glcE"
                     /locus_tag="PLW13_16345"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HQY72002.1"
                     /translation="MGADRDCSAEIAACVAAACGERTALAIRAGGTRDFYGRAVDGEI
                     LDVAAHRGVVAWEPAELVLTARAGTPLAGIEALLESEGQMLAFEPPRFGAASTLGGAI
                     AAAMSGPRRPYAGAARDFVLGVRCVNGRGELLGFGGQVMKNVAGYDVSRLMCGAFGTL
                     GVLLDISLKVLPVPPCEITVEHACDAAQAIVTMNRTAGSPLPLSASSHDGERLRLRLS
                     GAESAVRAAAATLGGDIVDDAAAYWKAVRDHTLEFFRAPGAVWRLSLPPATPPLALGG
                     EQFIEWGGALRWLRTTRDAAAVRAAVSATGGHATLYRGGDAANAEVFHPLAPGVEQLH
                     RRLKQAFDPEGILNPGRMYPWL"
     gene            complement(1231..>2124)
                     /locus_tag="PLW13_16350"
     CDS             complement(1231..>2124)
                     /locus_tag="PLW13_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQY72003.1"
                     /translation="GYDLLALLSGSEGMLGVIVEVTVRLLARPASASLLLAAFDDVER
                     AGSAVAAIIAAGIVPGGLEMMDNPALRAAEGFVHAGYPVEAAALLLCELDGTAEEVAE
                     QSRRVEELLRDSGAQQVRVARDERERALLWSGRKAAFPAVGRLSPDYYCMDGTIPRAQ
                     LGRVLRSIKELSLEHGLAVANVFHAGDGNLHPLILYDAAQPGELQRAEAFGARILELC
                     VACGGTITGEHGVGVEKIDQMCVQFRAPELAQFHAVKAAFDPAGLLNPGKAVPTLARC
                     AEFGRMHVHRGALAHPELERF"
BASE COUNT          317 a          814 c          718 g          275 t
ORIGIN      
        1 ccatcgagct cgtcgccgag caactggtag gtcggacagg tcgcggtgca gaagccgcag
       61 tgcacgcact tgcgcaggat cgcccgcgcc gcctcgccct cgggggtgtc gcggatgaaa
      121 tcggcgagcg gggcgtccat cgtgtctcag cgcctcgtca cagccacggg tacatgcgcc
      181 ccgggttgag gattccttcg gggtcgaagg cctgtttcag gcgccggtgc agctgctcca
      241 cgccgggcgc gaggggatga aagacttccg cgttcgccgc atcgccgccg cgatacagcg
      301 tcgcgtgccc gcccgtggcg ctgacagccg cgcgcaccgc cgctgcatcg cgcgtggtgc
      361 gcagccagcg cagggcgcca ccccactcga tgaactgctc gccgccgagg gcgagcggcg
      421 gcgtggccgg gggcagcgac agccgccaca ccgcaccggg cgcgcggaag aactccagcg
      481 tgtggtcgcg cacggccttc cagtacgccg cggcatcgtc gacgatgtca ccgcccagcg
      541 tggcggcggc cgctctcacc gcactttccg caccgctcag gcgcaggcgc agccgctcgc
      601 cgtcgtgact cgaggccgac agtggcaacg ggctgccggc cgtgcggttc atcgtcacga
      661 tcgcctgcgc ggcgtcgcag gcgtgctcga cggtgatctc gcacggcggc accggcagca
      721 ccttcagcga aatatccagc agcacgccca gcgtgccgaa ggcaccgcac atcaggcgcg
      781 acacatcgta gccggccacg ttcttcatca cctgcccgcc gaagccgagc agctcgccgc
      841 gcccgttcac gcaacgcacc ccgagcacga agtcgcgcgc cgcaccggca taggggcgcc
      901 gcgggccgga catcgcggcg gcgatcgcac cgcccagcgt ggacgccgcc ccgaaacgcg
      961 gcggctcgaa ggcgagcatc tgtccctcgc tctcgagcaa tgcctcgatc ccggcgagcg
     1021 gcgtgccggc gcgcgccgtg agcaccagtt ccgcgggttc ccacgcgacg acgccgcggt
     1081 gcgcggccac gtccaggatt tcaccgtcca ccgcacgacc gtagaaatcg cgcgtgccgc
     1141 cggcgcggat cgcgagcgcg gtgcgttcgc cgcaggcagc cgcgacgcag gccgcgatct
     1201 ccgcgctgca gtcgcgatcg gcgcccatgc tcaaaagcgc tccagctcgg ggtgcgcgag
     1261 agcgccgcgg tgcacgtgca tgcgaccgaa ctccgcgcag cgcgccagcg tgggcaccgc
     1321 tttccccgga ttcagcaggc cggcgggatc gaaggcggcc ttcacggcgt ggaactgcgc
     1381 cagttcgggc gcgcggaact gcacgcacat ctggtcgatc ttctcgacac ccacgccatg
     1441 ctcgccggtg atggtgccgc cgcacgccac gcagagctcg agaatgcgcg ccccgaacgc
     1501 ttccgcacgc tgcagttcgc cgggctgcgc ggcgtcgtag aggatcagcg gatgcaggtt
     1561 gccgtcgccg gcatggaaca cgttcgccac cgcgagcccg tgctccaacg acagttcttt
     1621 tatagagcgc agcacgcgcc cgagctgcgc acgcggaatg gtgccgtcca tgcagtagta
     1681 gtcgggcgac agccgcccca ccgcggggaa cgccgccttg cgccccgacc acagcagcgc
     1741 gcgctcgcgc tcgtcgcgcg ccacgcgcac ctgctgcgcg ccactgtcgc gcagcagttc
     1801 ttcgacgcgg cgcgactgct ccgcgacctc ctccgccgtg ccgtcgagct cgcacagcag
     1861 cagcgcggcg gcctccaccg ggtagcccgc gtgcacgaag ccctcggccg cgcgcagtgc
     1921 cgggttgtcc atcatctcga gcccgccggg cacgatgccc gcggcgatga tcgcggcgac
     1981 cgcgctaccc gcgcgctcca cgtcgtcgaa tgccgccagc agcaggctgg cgctcgcggg
     2041 cctggccagc aggcgcaccg tcacctcgac gatgaccccg agcattccct cggagccgct
     2101 cagcagcgcc agcaggtcgt aacc
//
LOCUS       DAORUQ010000343         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_83894_length_2091_cov_12.074656, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000343 DAORUQ010000000
VERSION     DAORUQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83894_length_2091_cov_12.074656"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2091)
                     /locus_tag="PLW13_16355"
     CDS             complement(<1..>2091)
                     /locus_tag="PLW13_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQY72004.1"
                     /translation="WLAGEEDEPQTLEGFRQAMASQPDEALEPEALWALADRHGYALE
                     LGYANADANADMDVLFRQRDAVIPGGVFWAQPADRPRATMANNPLKAKLVRDLNPRLR
                     QYLAGMLPEYMMPSAFVVLNELPLTPNGKIDRKALPAPGRTRVGVAVDYVAPRSETER
                     QIAEIWQQVLRLERVGVHDDFFELGGHSLLATQVVSRVRQALGAELPLRVLFAAPTVS
                     ALGERLAALLAGPQSALAAPPLVASDEAGPAKLSFAQQRLWILDQMAPGGSAYVMTGA
                     LELDGALDAGVLERALEALVARHEALRTVFVSEQDEPLQVVSKPQPWALPVTDLSGET
                     DARERLKERLREEAGRGFDLASGPLFRAQLYRLAPGTHVLLLAMHHIIYDGWSLGILY
                     RELGAIYGAFACGEAPALPVLRVQYRDFARWQRSWLQGEVLESQLSYWRERLTGAPMV
                     LALPADRARPPVESHRGAFHEFTLPRELVDRLQELSQREGATLFMSLLSGFALLLARY
                     SGQRDLLIGTPVANRNHAEVEPLVGFFVNTLVLRADLSGEPSARAFLGRMREACMDAY
                     AHQDLPFERLVEELHPARDLSRNAVFQVMFALQNAPMGALELLGLKLKPVEMGVGAAQ
                     FDLSLFAWETPDGLAARFSYASDLFDGSTIARMAAHWRLLLEAMVANPERRVDELPLL
                     AEAERRQVLVEWNAT"
BASE COUNT          363 a          792 c          647 g          289 t
ORIGIN      
        1 gtggcgttcc actccaccaa cacctgacgc cgctccgcct cggcaagcag cggcaattcg
       61 tcgactcggc gctcggggtt cgccaccatc gcctcgagca gcaagcgcca gtgcgccgcc
      121 atgcgcgcga tcgtcgaccc gtcgaacagg tcgctcgcat aagaaaaacg agcggcaagc
      181 ccgtccgggg tttcccatgc gaacagcgag agatcgaact gcgccgcccc aactcccatc
      241 tcgacgggct tcagcttgag ccccagcaac tccagcgccc ccatcggggc gttttgcagc
      301 gcgaacatca cctggaacac cgcgttgcgc gagaggtcgc gcgcagggtg cagctcctcc
      361 accagacgct cgaagggcag atcctggtgc gcataggcgt ccatgcacgc ctcacgcatg
      421 cggcccagaa acgccctcgc cgaaggctcg cccgagagat cggctcgcag caccagcgtg
      481 ttcacgaaga acccgaccag cggctcgacc tcggcgtggt tgcggttggc caccggcgtg
      541 ccaatcagga gatcgcgctg cccgctgtag cgcgcaagca gcagcgcgaa accggagagc
      601 aacgacatga acagcgtcgc cccctcgcgc tgcgacaatt cctgcaggcg atcaaccaac
      661 tcgcggggca acgtgaattc gtgaaacgcc ccgcggtggc tttccaccgg cgggcgcgcc
      721 cgatcggcgg gcaacgccag caccatcggc gccccggtca ggcgctcgcg ccagtacgaa
      781 agctgcgact ccagcacctc gccctgcaac cagctgcgct gccagcgcgc aaagtcacga
      841 tactgcacac gcagcaccgg cagcgccggc gcctcgccgc aggcgaaggc cccgtagatc
      901 gcgcccagct cccggtacag gatacccagc gaccagccat cgtagatgat gtggtgcatc
      961 gccagcagca gtacgtgcgt gccgggcgcc agccggtaga gctgcgcgcg gaacagcggc
     1021 ccgctcgcca gatcaaaccc gcgcccagcc tcctcgcgca agcgctcctt cagccgctcc
     1081 cgtgcgtccg tttccccgct caggtccgtg accggcaacg cccacggttg cggcttcgat
     1141 acgacctgca gcggctcgtc ctgctcgctc acgaacaccg tgcgcagcgc ctcgtggcgc
     1201 gccaccaggg cttccagcgc gcgctcgagc acccctgcgt caagcgctcc gtccagctcg
     1261 agcgcacccg tcatgacgta ggccgaccca cccggcgcca tctggtccag aatccacagc
     1321 cgctgctgcg cgaaggacag cttggctggg ccggcttcgt ccgacgccac caacggcgga
     1381 gcagcgagcg cggactgcgg acccgcgagc agcgcggcga gccgttcacc caatgccgaa
     1441 accgtgggcg ccgcgaacaa gaccctgagc ggcagttccg cgccgagagc ctggcgcacg
     1501 cgcgagacca cctgcgtggc gagcagcgag tgcccgccga gctcgaagaa gtcatcgtgc
     1561 accccgaccc gctcgagcct cagcacctgt tgccagatct cggcaatctg ccgctcggtc
     1621 tcgcttcgcg gcgcaacgta gtccacggcg acgccaacgc gggtgcgccc cggcgccggc
     1681 agcgccttgc ggtcgatctt gccgttgggc gtgagcggca attcgttgag caccacgaag
     1741 gccgagggca tcatgtactc gggcagcatg ccggcgaggt attggcgcag tcgagggttc
     1801 aagtcgcgca ccagcttcgc cttcagcgga ttgttcgcca tcgtcgcccg gggcctgtcc
     1861 gccggctgcg cccagaacac gccacccgga atcaccgcgt cgcgctgccg gaacaacacg
     1921 tccatgtccg cattggcgtc agcgttggcg taccccagct ccagcgcgta gccgtgccga
     1981 tcggcgagcg cccaaagcgc ctcgggctcc agcgcctcgt cgggctgcga ggccatcgcc
     2041 tgcctgaagc cttccagcgt ctgcggctcg tcttcttctc cggccagcca g
//
LOCUS       DAORUQ010000344         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_89005_length_2019_cov_4.350305, whole genome shotgun sequence.
ACCESSION   DAORUQ010000344 DAORUQ010000000
VERSION     DAORUQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89005_length_2019_cov_4.350305"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLW13_16360"
     CDS             <1..1025
                     /locus_tag="PLW13_16360"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY72005.1"
                     /translation="SPALTDFVVMSEGSTMFLTGPEVVRSVTGEQVSAQDLGGAAVHM
                     GRSGVAHLSAADDAAALERVKQLLGYLPQNNSEDPPPITPYDPAERMDAALDCIVPET
                     ESQAYDMRDVLGHVFDRASVLELQPDYAPNALVAFARLDGFPVGVVANQPAHLAGALD
                     IDASDKIARFIRCCDAYNIPVVSFVDCPGFLPGSEQESRGVIRHGAKIIYAYCEATVP
                     KISIVTRRAMGGAYVAMGSKQMRTDLAFAWPTAQIAVMGAEPAVHVLYRKEVREAPDP
                     DARVAELMSDYREKFFNPYRAADLGQVDEVIEPRETRPRLIRALEVLRTKVQQSPPKK
                     HGLPPN"
     gene            1040..1417
                     /locus_tag="PLW13_16365"
     CDS             1040..1417
                     /locus_tag="PLW13_16365"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HQY72006.1"
                     /translation="MENLGWGLQTTLLGMGLVFAMLALLWGLLVLLLRLDGPGQSPLQ
                     PPVPAGADVAPADGMPAAGAPGMPDPALRAAITVAVLWHRQVLRGEAAPAVRLHPPGA
                     LLHASRWVDAGRMRQNRVWRRNR"
     gene            1421..1876
                     /locus_tag="PLW13_16370"
     CDS             1421..1876
                     /locus_tag="PLW13_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72007.1"
                     /translation="MHRYTIEIDGRRFIIDVEERGAECFAVQVDGQSFDVTLAGSEEL
                     GSEGAVRLEPSAAPAAMNVTLPSVPATPPATANNGVLRAPMPGVILRVLVAPGARVSR
                     GQDLAVLEAMKMENIIRAPQDATVAEVLVRPGQHVGHGAALVRLEAPTP"
BASE COUNT          298 a          715 c          711 g          295 t
ORIGIN      
        1 attccccggc gctgaccgac ttcgtggtga tgtccgaggg ctcgaccatg ttcctcaccg
       61 ggccggaggt ggtgcgctcg gtgaccggcg agcaggtcag cgcgcaggac ctgggcggcg
      121 cggcggtgca catggggcgc agcggcgtgg cccacctctc ggcggccgac gacgccgcgg
      181 cgctggagcg cgtcaagcaa ctgctgggct acctgccgca gaacaacagc gaggacccgc
      241 ctccgatcac cccctacgac ccggccgagc gcatggacgc ggcgctggac tgcatcgtcc
      301 ccgagacgga gagccaggcc tacgacatgc gcgacgtgct gggacatgta ttcgatcgcg
      361 ccagcgtact ggaactgcag cccgactacg cgcccaacgc gctggtcgcc tttgcccggc
      421 tcgacggttt cccggtcggc gtggtggcca accagcccgc gcacctcgcc ggtgcgctgg
      481 acatcgacgc cagcgacaag atcgcgcgct tcatccgctg ctgcgacgcc tacaacattc
      541 ccgtcgtcag cttcgtcgac tgtcccggct tcctgccggg cagcgaacag gagtcgcgcg
      601 gcgtgatccg ccacggcgcc aagatcatct atgcctattg cgaagccacg gtacccaaga
      661 tctccatcgt cacgcgccgc gccatgggcg gggcctacgt ggccatgggc agcaagcaga
      721 tgcgcaccga tctcgcgttc gcctggccca cggcccagat cgcggtgatg ggcgccgagc
      781 cggcggtgca cgtgctgtac cgcaaggaag tgcgcgaggc gcccgatccc gacgccaggg
      841 tggccgagct gatgtccgat taccgcgaga agttcttcaa cccctaccgc gccgcggacc
      901 tggggcaggt ggatgaggtg atcgaaccgc gcgagacgcg accgcggctg atacgcgccc
      961 tggaggtgct tcgcaccaag gtgcagcaat cgccgccgaa gaagcatggc ctgccaccga
     1021 actgaaggag ggttgcgcga tggagaacct gggctgggga ctgcagacca cgctgctcgg
     1081 catgggcctg gtattcgcga tgctggcgct gctgtggggg ctgctcgtgc tgttgctgcg
     1141 gctcgacggc ccggggcagt cgccgctgca gccgccggtg cccgcaggcg ctgacgtagc
     1201 gcctgccgat ggcatgcccg cggcaggcgc acccgggatg ccggaccccg cgctgcgtgc
     1261 ggccatcacc gtggccgtgc tttggcatcg ccaggtgttg cgcggcgagg ccgctcccgc
     1321 cgtgcgcctt caccctcccg gcgccctgct gcatgcctcg cggtgggtgg acgccggtcg
     1381 catgcgccag aaccgtgtct ggcgcaggaa ccgctgaacc atgcaccgct acaccatcga
     1441 gatcgacggc cggcgcttca tcatcgacgt cgaggaacgg ggcgccgagt gcttcgccgt
     1501 gcaggtcgac ggacaatcgt tcgacgtcac cctggcgggc agcgaagagc tcggcagcga
     1561 gggtgccgtg cggctcgagc cctcggctgc gccagcggcc atgaacgtca cgctgccatc
     1621 cgtaccggct acgccgccgg ccaccgcgaa caacggcgtg ctgcgcgccc ccatgcccgg
     1681 cgtcatcctg cgcgtgctgg tcgctcccgg cgcacgcgtg agccgcggcc aggacctggc
     1741 ggtactcgag gcgatgaaga tggagaacat catccgcgcg ccgcaggacg ccacggtcgc
     1801 cgaggtgctg gtcaggcccg ggcagcacgt cgggcacggc gcggccctcg tgcgcctcga
     1861 ggcgccaacc ccgtgagcgc cgccagcctg tccgcgatga cgcagggcct gctggcggtc
     1921 acgccgcagt cgctggcgat gatcgcggtg gcgctgggcc tgttgtatct cggcatcgcg
     1981 cggcgctacg agccgctgct gctggtgccg atcgcggcg
//
LOCUS       DAORUQ010000345         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_89066_length_2018_cov_4.612328, whole genome shotgun sequence.
ACCESSION   DAORUQ010000345 DAORUQ010000000
VERSION     DAORUQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89066_length_2018_cov_4.612328"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLW13_16375"
     CDS             <1..911
                     /locus_tag="PLW13_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY72008.1"
                     /translation="AAKRARATPRGAAAKPVATARPRAGAKPRSGRRPRRRGLAGRLL
                     ALWPTLQARRRWARRIAAMPSAVLILAVLAFVVGGWAVVNTVYHVARKPTELLFPVSD
                     ALYKTPAETWAAYGELFRENSTAVITPELLAALAQIEASGNPVARTYWRWSFTADPLD
                     IYRPASSAVGMFQFTDGTFEEARRYCIRDNRVVEDGPWDDFDSCWFNGLYFRVLPSHS
                     VELTAAYLDRRVAGVLARHRLRNVPLAKKHELATLIHLCGAGAGNAFARRGLSLRPGQ
                     RCGAHDARAYLDKVARMKAVFARLAR"
     gene            1009..1812
                     /locus_tag="PLW13_16380"
     CDS             1009..1812
                     /locus_tag="PLW13_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY72009.1"
                     /translation="MSHTTPSSFRPDLLAGQVAFVTGGATGIGKEICRVLGRHGARIA
                     MLSRREDRLRAAVEELQGEGIEAHYAVGDVREPQAVEAAVRGILARFGRIDILVNNAA
                     GNFPAPMSQISPNGFRSVVAIDLLGTYNVSRAVFDAWQREHGGNIVNISAPFELKGAA
                     LQAHVAAAKAGVDSLTRTCAVEWGPYGIRVNAVAPGQIEQTEGMQRFAAAVEDSGPGS
                     PLGITGHGSDIAHAVLFFCSPAARFISGQVIAVDGAATVDQLKLGLGRV"
BASE COUNT          285 a          712 c          720 g          301 t
ORIGIN      
        1 ccgcggcgaa gcgggcgcgc gccacaccca ggggggctgc cgcgaagccg gtcgccaccg
       61 cacgcccgcg tgcgggcgcg aagccgcggt ccgggcgccg cccccggcgc cgggggcttg
      121 ccgggcgcct gctggccctg tggcccacgt tgcaggcgcg tcggcgctgg gccaggcgca
      181 tcgccgcgat gccctcggcc gtgctgatac tggccgtgct ggcgttcgtg gtcggcggct
      241 gggccgtggt caacaccgtc taccatgtgg cgcgcaaacc gaccgagctg ctctttccgg
      301 tcagcgatgc gctgtacaag acgccggccg aaacctgggc cgcctacggc gagctgtttc
      361 gcgagaattc caccgcggtg atcacgcccg agttgctcgc ggcgctggcg cagatcgagg
      421 cctcgggcaa cccggtggcg cgcacctact ggcgctggtc gttcacggcg gatccgctcg
      481 acatctaccg cccggcctcg agcgcggtgg gcatgttcca gttcaccgac ggcaccttcg
      541 aggaagccag gcgctactgc atccgcgaca accgcgtggt ggaggacggg ccgtgggacg
      601 atttcgattc gtgctggttc aacgggctgt acttccgcgt gctgcccagc cattcggtcg
      661 agctgaccgc cgcctacctc gaccggcgcg tcgcgggcgt gctggcgcgt catcggctgc
      721 gcaatgtgcc gctcgcgaag aagcacgagc tcgcgaccct gatccatctc tgcggagcgg
      781 gcgcgggcaa cgcgttcgcg cgccgcggtc tcagcctgcg ccccgggcag cgctgcggcg
      841 cgcacgacgc gcgcgcctac ctcgacaagg tcgcgcgcat gaaggcggtg ttcgcgcgcc
      901 tggcgcgctg agcctgtctc gcttgtgggt tcggcttcag ccggacatta tctggcgagc
      961 gcgggcatcg gaatgtcagg ctgaagcctg acccacagag ggaaaatcat gagccatacg
     1021 acaccctcga gcttccgccc ggaccttctc gccgggcagg tcgccttcgt gaccggcggc
     1081 gccaccggca tcggcaagga gatctgccgc gtgctcgggc gccacggcgc gcgcatcgcg
     1141 atgctgagtc gcagggaaga caggctcagg gcggcggtcg aggagttgca gggcgaaggc
     1201 atcgaggcgc actacgcggt cggcgacgtg cgcgaaccgc aggccgtgga ggccgcggtg
     1261 cgcggcatcc tggcgcgctt cgggcgcatc gacatactcg tgaacaacgc ggcgggcaat
     1321 ttcccggcac cgatgagcca gatctcgccg aacggctttc gctccgtggt ggccatcgac
     1381 ctgctcggca cctacaacgt ctcgcgcgcg gtgttcgacg cctggcagcg cgagcacggc
     1441 ggcaacatcg tcaacatctc ggcgcccttc gagctcaagg gggcggcgct gcaagcgcac
     1501 gtcgcggcgg ccaaggccgg cgtggattcg ctgacgcgca cctgcgccgt cgagtggggg
     1561 ccctacggca tccgcgtgaa cgcggtggcg ccgggccaga tcgagcagac cgagggcatg
     1621 cagcgcttcg cagcggcggt ggaggattcc ggtcccggca gcccgctcgg catcacgggc
     1681 cacggcagcg acatcgccca tgcggtgctg ttcttctgct cgccggcggc ccgtttcata
     1741 tcggggcagg tgatcgccgt cgatggcgcc gccacggtcg accagctcaa gctggggctg
     1801 gggcgggtct gaacgccgcc ttacgattct gtcatcgagg ccgcgtccgc gccgcccgac
     1861 aatggcgcct cgagccacac gcccggaatc cacgccgatg cccagcttca ccgagatcgc
     1921 cagcggcctg cagttcccgg aaggacccat cgcgctgccc gacggcagcg tgctgctggt
     1981 cgagatccgc cgcggcacat tgagccgcgt gctgcccg
//
LOCUS       DAORUQ010000346         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_92750_length_1970_cov_6.702872, whole genome shotgun sequence.
ACCESSION   DAORUQ010000346 DAORUQ010000000
VERSION     DAORUQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92750_length_1970_cov_6.702872"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PLW13_16385"
     CDS             complement(<1..1261)
                     /locus_tag="PLW13_16385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY72010.1"
                     /translation="MSLRRLCIAALALLWLAPGAGMAMSEKKELEIGREMHQEILAKM
                     PVYANAAVADYVGRIGQQLARDSDRPGLEYTFTVLDSPDINAFATPGGYVYINRGLLA
                     YLQSEAELAAVLAHEIAHITERHASRQDVMAKTSSVASLALGVLAAIGTGSGAVGGVA
                     QDAASSAGTAMVRGYGRDMELEADREGAKFMHRNGYDPLAMIEVLGALKEQESFSRLR
                     AKEAGRRPAVYHGVFSTHPRNDERLKEVIAAAGEMSPGAQRAIDADEFRRVTEGMKFS
                     EQPRAAAVIDNRYYNGKMGFTLAFPTGWKVVNRASTVLAGPERDNTMLQMSVKRALPE
                     MTPAEFASGMLNLQGAKGGEEIADGEVKGYTALYPGAAGAPGRRIAVLYFGPYAYVFE
                     GRTANASLAAFYDTMFLSAIRSFRPMSG"
     gene            complement(1258..1920)
                     /locus_tag="PLW13_16390"
     CDS             complement(1258..1920)
                     /locus_tag="PLW13_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72011.1"
                     /translation="MRSVAVRLRWLAVALCVALGAACTTTRIDESRVESAAVGSGEKV
                     VVLGRRHNSEYETEPEFVRCVGRGVASTGAAVVPELEFMNSFYPWFEPRTAPMSLKRF
                     EALLQMPAFATRMRELNLRYIVWVDGRTETVDKAGSISCAVGPGGAGCIGFGTWDDES
                     KYEASVWDLRLSQSIATISTESAGTSYMPAVIVPVPIIARVRAAACEGLSAQLKKLFS
                     PA"
BASE COUNT          298 a          705 c          659 g          308 t
ORIGIN      
        1 caccgctcat ggggcggaag ctgcggatcg cggacaggaa catggtgtcg tagaaggcgg
       61 ccagcgaggc attcgcggtg cggccctcga acacgtaggc ataggggccg aaatacagca
      121 ccgcgatgcg ccgtcccggc gcgccggcgg cgcccggata cagcgccgta tagcccttga
      181 cctccccgtc cgcgatctcc tcgccgccct tggcgccctg caggttcagc atgccggagg
      241 caaactcagc cggcgtcatc tcgggcagcg cgcgcttgac cgacatctgc agcatcgtgt
      301 tgtcgcgctc gggacccgcg agcaccgtgc tggcgcggtt cacgaccttc cacccggtgg
      361 ggaacgccaa cgtgaaaccc atcttgccgt tgtagtagcg gttgtcgatc acggccgcgg
      421 cgcgcggctg ctcgctgaat ttcatgccct cggtgacgcg gcggaactcg tcggcatcga
      481 tcgcgcgctg cgcgccggga ctcatctcgc cggcggcagc gatcacttcc ttcaggcgtt
      541 cgtcgttgcg cgggtgcgtg gagaacacgc cgtggtagac cgcgggcctg cgaccggcct
      601 ccttcgcccg caaccgcgag aagctctcct gctccttcag cgcgccgagc acctcgatca
      661 tcgccagcgg atcgtagccg ttgcggtgca tgaacttcgc gccctcgcgg tcggcctcga
      721 gctccatgtc gcgaccgtag ccgcgtacca tcgcggtgcc cgcgctggaa gccgcgtcct
      781 gcgccacgcc accgaccgcg ccgctgccgg tgccgatcgc cgccagcacg ccgagcgcga
      841 gcgaggccac cgagctggtc ttggccatca cgtcctggcg cgaggcgtgg cgctcggtga
      901 tgtgggcgat ctcgtgcgcg agcacggcgg ccagctcggc ctcggactgc aggtaggcga
      961 gcaggccgcg gttgatgtag acgtagccgc ccggggtcgc gaaggcgttg atgtccggac
     1021 tgtcgagcac ggtgaatgtg tattcaaggc cgggccggtc gctgtcgcgc gccagttgct
     1081 ggccgatccg cccgacgtag tcggcgaccg ccgcgttggc gtacaccggc atcttcgcga
     1141 ggatctcctg gtgcatctcg cggccgatct cgagctcctt cttctcggac atcgccatac
     1201 cggcacccgg cgccagccac agcagcgcca gcgcggcaat gcagagccgg cgcaggctca
     1261 tgcgggcgag aagagcttct tcagctgcgc ggagagcccc tcgcaggcag cggcgcgcac
     1321 gcgcgcgatg atcggcaccg gcacgatcac ggccggcatg taggaggtgc ccgccgactc
     1381 ggtgcttatg gtggcgatgg attgcgacag gcgcagatcc cacacgctgg cctcgtactt
     1441 cgactcgtcg tcccaggtgc caaaaccgat gcagccggcg cctccggggc ccacggcgca
     1501 ggaaatcgag cccgccttgt cgacggtctc ggtgcgcccg tcgacccaca cgatgtagcg
     1561 caggttcagt tcgcgcatgc gcgtggcgaa ggccggcatc tgcagcagcg cctcgaagcg
     1621 cttgaggctc atcggcgcgg tgcgcggctc gaaccagggg tagaagctgt tcatgaactc
     1681 gagctcgggc accaccgcgg cgccggtgga cgcgacaccg cgtcccacgc agcgcacgaa
     1741 ctcgggctcg gtctcgtact cgctgttgtg gcgacgtccc agcaccacca ccttctcgcc
     1801 gctgcccacg gccgcggact cgacccggct ctcgtcgatg cgggtcgtgg tgcaggccgc
     1861 gccgagcgcc acgcacagcg ccaccgccag ccagcgcagc cgtaccgcaa cactcctcat
     1921 gggcgactcc cctgctcctc gctcatggtc acatcataag accggaacgg
//
LOCUS       DAORUQ010000347         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_92827_length_1969_cov_6.689655, whole genome shotgun sequence.
ACCESSION   DAORUQ010000347 DAORUQ010000000
VERSION     DAORUQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92827_length_1969_cov_6.689655"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLW13_16395"
     CDS             complement(<1..485)
                     /locus_tag="PLW13_16395"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938182.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HQY72012.1"
                     /translation="MNTRINRHGLQIAQPLHDLVVDEILPGTGIDADALWRGFAAIVR
                     EFAPRNGAVLARRDGLQAQIDAWHRAHPAFDAAAYRDFLVGIGYLLPEGEDFTIGTQN
                     VDAEIATLAGPQLVVPVMNARYALNAANARWGSLYDALYGTDVIPEDGGATRAGPYNP
                     VR"
     gene            complement(610..1566)
                     /locus_tag="PLW13_16400"
     CDS             complement(610..1566)
                     /locus_tag="PLW13_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY72013.1"
                     /translation="MNIGRIDLNLLVYLDVLLRERNVTRAASYLGITQPAMSNGLRRL
                     RELFDDPLLVRTSEGMTPTERALELQPVVRSVLAAVERAVQPKKPFDAARSTRVFRIM
                     ASDYAESTLIPAVLERLQREAPDIALDIMTPSDVSYLNVEQGKVDMVINRFDSMPQSF
                     HQNTVWQDSFSCLLSVDNPLLAGGFDLESYLHAQHVWVSKTGMGVGVGVDPSAAQDLG
                     WVDEALARLGRKRRITVFTRHYQVAVLLATQKHLVVTVPSKLALLMRDNPRVAIKPPP
                     FEIPPFELKMAWSPLLQRNPGHQWMRRLIGEVAREIDGDTAA"
     gene            1690..>1969
                     /locus_tag="PLW13_16405"
     CDS             1690..>1969
                     /locus_tag="PLW13_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HQY72014.1"
                     /translation="MSIHTSTRAEQIALLEKDWAENPRWKGVKRGYSAADVVRLRGSV
                     KVEHTLATRGAEKLWNLVNTEPFVNTLGALTGNQAMQQVKAGLKAIYLS"
BASE COUNT          319 a          641 c          677 g          332 t
ORIGIN      
        1 cgcaccgggt tgtaggggcc ggcgcgcgtg gctccgccgt cctcggggat cacgtcggtg
       61 ccgtagagcg cgtcgtagag actgccccag cgcgcgttcg ccgcgttcag cgcgtagcgc
      121 gcgttcatca ccggcaccac cagctgcggt ccggccagcg tggcgatctc ggcatcgacg
      181 ttctgcgtgc cgatcgtgaa gtcctcgccc tcgggcagca ggtagccgat cccgaccagg
      241 aagtcgcggt aggcggcggc atcgaaggcg gggtgcgcgc ggtgccaggc gtcgatctgc
      301 gcctgcagcc cgtcgcggcg cgccagcacg gcgccgttgc gcggcgcgaa ctcgcgcacg
      361 atggcggcga agccgcgcca gagagcgtcg gcatcgatgc cggtgccggg caggatctcg
      421 tcgaccacca gatcgtgcag gggctgggcg atctgcaggc cgtgccggtt gatgcgtgtg
      481 ttcatctggg ggctcctctg gtgcggtgtg cggtttcgcg gcggggcagg cggcggattc
      541 taggtcctcg ctcccggcgc gacagggtta tgcagatgat tcccggtatt cacaagatga
      601 atgcattgtt caggctgccg tgtcgccgtc gatctcgcgc gcgacctcgc cgatcaggcg
      661 ccgcatccac tggtgtccgg gattgcgctg cagcagcggg ctccaggcca ttttcagctc
      721 gaacggcgga atctcgaagg gcggcggctt gatcgcgacg cgcgggttgt cgcgcatcag
      781 cagcgcgagc ttgctcggca ccgtgaccac caggtgcttc tgcgtcgcca gcagcaccgc
      841 cacctggtag tggcgggtga ataccgtgat gcggcgcttg cgcccgagcc gcgccagcgc
      901 ctcgtccacc cagccgagat cctgcgcggc gctgggatcc acgcccacgc ccacgcccat
      961 gccggtcttg ctgacccaga catgctgggc atgcaagtag gattcgaggt cgaaaccgcc
     1021 cgcgagcaac gggttgtcca cgctcagcag gcaggaaaaa ctgtcctgcc agacggtgtt
     1081 ctggtggaaa gactgcggca tcgagtcgaa gcggttgatc accatgtcga ccttgccctg
     1141 ctcgacattc aggtagctca cgtcactcgg tgtcatgatg tcgagcgcga tgtcgggggc
     1201 ctcgcgctgc aggcgctcca gcaccgcggg gatcagcgtg gattccgcgt agtcgctggc
     1261 catgatgcgg aacacgcgcg tggagcgcgc ggcatcgaag ggcttcttgg gctgcaccgc
     1321 gcgctcgacc gccgccagca cgctgcgcac cacgggctgc agttccagtg cccgctcggt
     1381 gggtgtcatg ccctcgctgg tgcgcaccag cagggggtcg tcgaagagtt cgcgcagccg
     1441 gcgcagcccg ttgctcatcg cgggctgggt gatgccgaga tagctggccg ctcgcgtgac
     1501 gttgcgttcc cgcagcagca cgtcgagata tacgagcagg ttgaggtcta ttcgtcctat
     1561 attcatcgtc ggaatggcgg agataggaat gataaattgg atagattatc ccgccatccc
     1621 tagaatccgt ccaccttcac gacacctccg ggcgaacccc ggggccaacc acacccaagg
     1681 gagcatccca tgagcatcca tacctccacc cgtgccgagc agatcgcact cctcgagaag
     1741 gactgggccg agaacccgcg ctggaagggc gtcaagcgcg gctacagcgc cgccgatgtg
     1801 gtgcgtctgc gcggctcggt gaaggtcgag cacacgctcg cgacccgcgg tgccgagaag
     1861 ctctggaacc tcgtgaacac cgagccgttc gtcaacacgc tcggcgcgct gacgggcaac
     1921 caggcgatgc agcaggtcaa ggccgggctg aaggcgatct acctgtcgg
//
LOCUS       DAORUQ010000348         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_93443_length_1961_cov_7.720357, whole genome shotgun sequence.
ACCESSION   DAORUQ010000348 DAORUQ010000000
VERSION     DAORUQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93443_length_1961_cov_7.720357"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..467)
                     /locus_tag="PLW13_16410"
     CDS             complement(3..467)
                     /locus_tag="PLW13_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY72015.1"
                     /translation="MPLRFNHMELTLPKGALTHEREAITGFYGQVFGFGAIDVPLFDQ
                     TGLLLQSDPETSHFILLMEQDEPLRSPGYDHLGFLLDSRAEVDAKLALCKEWQARDPR
                     VQIKEYEDLVIGDVVTHAFYFRYILPIWFDVQVIEYGSAEHAPKHAWVYRPV"
     gene            complement(541..750)
                     /locus_tag="PLW13_16415"
     CDS             complement(541..750)
                     /locus_tag="PLW13_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQY72016.1"
                     /translation="MNEDNDACRAARATSPCRRQCTLDERDVCMGCGRTITEICGWSA
                     LDEAGRIRVCADAAARLAARERRAR"
     gene            873..1877
                     /locus_tag="PLW13_16420"
     CDS             873..1877
                     /locus_tag="PLW13_16420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF009098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQY72017.1"
                     /translation="MNQLKVGLLGGGSWGTTVASLVARNAPAVLWARDPATVAEINAR
                     HTNAKYLPGAKLHENLRASVDIAEASAEVDVVVVGVPSQGFRGALEAIRAHIRPWVPV
                     ISLTKGLEAGSGKRMTEIIAEVLPGHPAGVLTGPNLAREVMSGFAAASVIAMEDQTIN
                     RALQQLFSSGLFRVYTNDDVIGCELGGVLKNVIAIAVGMGDGQGAGDNTRAALICRGL
                     AEITRLGVAMGGKPATFAGLAGVGDLVATCTSAQSRNRHVGVELGKGRHIDEIIAGMF
                     MVAEGVKSAPVVMELAQHHNVDMPLVREVFDVVQGRANARRAFRGLLRIAAGAESEAG
                     "
BASE COUNT          315 a          657 c          678 g          311 t
ORIGIN      
        1 acctacacgg gccggtacac ccacgcatgt ttcggcgcgt gctcggcgct gccgtactct
       61 atcacctgca cgtcgaacca gatcggcagg atgtagcgga agtagaaggc gtgcgtgacc
      121 acgtcgccga tgacgagatc ctcgtactcc ttgatctgca cgcgcggatc gcgcgcctgc
      181 cattccttgc acagcgcgag cttcgcgtcg acctcggcac ggctgtcgag caggaagccg
      241 aggtggtcgt aacccggcga gcgcagcggc tcgtcctgct ccatcagcag gatgaagtgg
      301 ctggtctcgg gatcgctctg caacagcagt ccggtctggt cgaacagcgg cacgtcgatc
      361 gcgccaaagc cgaagacctg cccgtagaag cccgtgatcg cctcgcgctc gtgcgtgagc
      421 gcgcccttgg gcagcgtcag ttccatgtgg ttgaagcgca gcggcatcgg aacggttcct
      481 cgtgttttac tggtgcgccg cagtgtacgc gcaatgccgc gcgcactgga tggcgattcg
      541 ttaacgcgcc cggcgctccc gcgccgcgag ccgggcggcg gcatcggcgc acacgcgaat
      601 gcggccggcc tcgtcgagcg cggaccagcc gcagatctcg gtgatcgtgc gtccgcagcc
      661 catgcagacg tcgcgttcat ccagcgtgca ctggcgccgg cacggcgagg tagcgcgcgc
      721 cgcccggcac gcgtcattgt cttcgttcat cgtcgccccc tttttccctg gcggacttta
      781 tacgctgcgg cgggcgacgc aaggccgtgc ttgcggcgcc cggaggatgc gcgcaatact
      841 tcaccgcgaa ccgtccggaa caacacgccc tgatgaacca actcaaagtc ggactgctcg
      901 gtggcggctc gtggggcacc acggtggcct cgctggtcgc gcgcaacgcg ccggcggtgc
      961 tgtgggcgcg cgatcccgcg accgttgcgg agatcaacgc gcggcatacc aatgccaaat
     1021 accttccggg cgcgaagctg cacgagaatc tgcgcgcgag cgtcgacatc gccgaggcga
     1081 gcgccgaggt cgacgtggtc gtggtcggcg tgccctcgca gggattccgc ggtgcactcg
     1141 aggcgatccg cgcgcacatc cgcccctggg tgccggtgat cagcctcacc aaggggctcg
     1201 aggccggcag cggcaagcgc atgaccgaga tcatcgccga agtgctgccg gggcacccgg
     1261 ccggcgtcct gacggggccg aacctagccc gcgaggtgat gtcgggtttc gccgccgcca
     1321 gcgtgatcgc gatggaggac cagaccatca accgcgcgct gcagcagctg ttcagcagcg
     1381 ggcttttccg cgtttacacc aacgacgacg tgatcggttg cgaactgggc ggcgtgctga
     1441 agaacgtcat cgccatcgcg gtcggcatgg gcgacggcca gggcgcgggc gacaacacgc
     1501 gcgcggccct gatctgccgc gggctggcgg aaatcacgcg gcttggcgtc gcgatgggcg
     1561 gcaagcccgc gacctttgcc ggtctcgcgg gcgtgggcga cctggtggca acctgcacca
     1621 gcgcgcagag ccgcaaccgt cacgtcggcg tggaactcgg caagggccgc cacatcgacg
     1681 aaatcatagc cggcatgttc atggtcgccg agggcgtgaa gagcgcgccg gtggttatgg
     1741 aactggcgca acaccacaac gtcgacatgc cgctggtacg cgaggtcttc gacgtggtgc
     1801 agggccgcgc caatgcgcgc cgcgccttcc gcgggctgct gcgcatcgcg gccggcgcgg
     1861 aatccgaagc cggctgatgc gcgttcggct tcagccgggc aaccgtgggt ccggattcat
     1921 ccggacaatc gcaggcccgg attctttcgg gcatcgtccg g
//
LOCUS       DAORUQ010000349         1922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_96722_length_1922_cov_6.524371, whole genome shotgun sequence.
ACCESSION   DAORUQ010000349 DAORUQ010000000
VERSION     DAORUQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96722_length_1922_cov_6.524371"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1846)
                     /locus_tag="PLW13_16425"
     CDS             complement(77..1846)
                     /locus_tag="PLW13_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HQY72018.1"
                     /translation="MFLRATSRKKDGKVHRYYSVVENRRVAGGRVVQRHVLYLGEINS
                     SQERAWRRSIEVVEDGAEAPRTVALFPEDRIEGLLADASIVTIRVSAMRLKRPRQWGA
                     CWLALTLWQRLDLDVFFAERLPRSRKGTRWDLAVLVLAAYRLISPGSEWRLHREWYGR
                     TALADLLGSDDGLVDPHVLYRVHDRLLAHKTALFDHLTHRWRDLFNARYEVLLYDLTS
                     TYFESDPPLDDADPRRHGYSRDHRPDCVQIVIALIVTPEGLPIAYEVLPGNTADSTTL
                     KGFLAQIEQQYGKAQRIWCMDRGIPTEAVLEQMRASDPPVQYLVGTPKGRLNRLESAL
                     VTKPWCQAREGVKVKLLPEDGELYVYAESRDRIAKERSMRQRLLKWLWARLAALREMD
                     LTRDALLMKLGHAQSKAPAAWRLIDLSVQANAASFTYRLNRAKLRQVRRREGRYLLRT
                     NLTETDPVKLWEYYLQLVRVEEAFRTLKSDLAIRPIYHQHPSRIEAHVFLSFLAYCLY
                     VTLGRTLHALAPGLTARSALEKFAAMQMVDVHLPTTDGRELILSRYTEPERELALLLE
                     RLELTLPAQPKPRITAAEAATAL"
BASE COUNT          325 a          668 c          608 g          321 t
ORIGIN      
        1 ccgatcttcg ccgattcgcg gggtagatga gggtcaagtc gttgatttgt catgaacgca
       61 cccccttagg tctgcactac agggcggttg cggcctcagc ggcggtaatc cgcggcttgg
      121 gctgcgcggg caaggtcagt tcgagccgct cgagcagcag cgcgagctcg cgctccggct
      181 cggtgtagcg cgagaggatc aactcgcgcc cgtcggtggt cggcaggtgc acgtcgacca
      241 tctgcatcgc cgcgaacttc tcgagcgcgc ttctcgccgt cagtccgggc gccagcgcgt
      301 gcagggttcg cccgagcgtc acgtacaggc agtacgcgag gaaggagaga aacacgtgcg
      361 cctcgatccg cgaggggtgc tggtggtaga tcgggcggat cgccagatcg ctcttcagcg
      421 ttcggaacgc ctcctcgacc cgcaccagct gcaggtagta ctcccacagc ttcaccggat
      481 cggtctcggt gaggttggtg cgcaggagat agcggccttc gcgccgccga acctgcctga
      541 gcttcgcgcg gttcagccgg taggtaaacg aggcggcgtt cgcctgcacg ctgaggtcga
      601 tcaaccgcca cgccgcgggc gccttcgatt gcgcatgacc gagcttcatc aacaacgcat
      661 cgcgtgtcag gtccatctcg cgcagcgcgg ccaggcgcgc ccacaaccac ttcaggagcc
      721 gctgacgcat cgagcgttct ttggcgatcc ggtcgcggct ctcggcgtac acgtagagtt
      781 caccgtcctc gggcaagagc ttgaccttga cgccctcgcg cgcctggcac cacggcttgg
      841 tgacgagcgc ggactcgagg cggttcagcc gtcccttggg cgtgccgacc agatactgca
      901 ccggcggatc cgaggcacgc atctgctcga gcactgcttc ggtcggaatg ccgcggtcca
      961 tgcaccagat gcgctgcgcc ttgccatact gctgctcgat ctgcgcgaga aaccccttca
     1021 gcgtcgtgga atcggccgtg ttgcccggca gcacttcgta ggcgatcggt agcccctccg
     1081 gcgtgacgat cagcgcaatc acgatctgca cgcaatcggg gcgatgatca cggctatagc
     1141 cgtgccggcg cgggtccgca tcgtccaggg gcggatcgga ctcgaagtag gtcgaggtca
     1201 gatcgtacag cagcacctcg tagcgggcat tgaacaggtc acgccagcgg tgcgtcagat
     1261 gatcgaacag cgccgtcttg tgcgcgagca accggtcgtg cacgcggtag agaacgtggg
     1321 ggtcaacgag tccgtcgtcg ctgcccagca ggtcggccag cgcggtgcgc ccgtaccact
     1381 cccgatgcaa ccgccactcg ctgcctgggc tgatcagccg gtacgcggcc agcaccagca
     1441 ccgccaggtc ccagcgcgtg cccttgcgcg agcgcggcag ccgctcggcg aaaaacacgt
     1501 cgagatcgag ccgctgccac agcgtcaacg ccagccagca tgcgccccac tgccgcgggc
     1561 gcttcaggcg catcgccgac acgcggatcg tcacgatcga cgcatccgcc aacagcccct
     1621 cgatccgatc ctccgggaac aacgccacgg tgcgcggcgc ctcggccccg tcctcaacta
     1681 cctcgatcga gcggcgccac gcccgttcct ggctcgagtt gatctcgccc agatacaaga
     1741 cgtgccgctg caccacgcgc ccaccggcca cacgccggtt ctccaccacg ctgtagtagc
     1801 ggtgaacctt gccgtccttc ttgcgggatg tcgcgcgcag aaacatcgcg actcgccatt
     1861 atccgcatgt ccacgacgtg cgccactgga aggtcttcac tacagccact tccgccggct
     1921 cc
//
LOCUS       DAORUQ010000350         1920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_96903_length_1920_cov_5.843968, whole genome shotgun sequence.
ACCESSION   DAORUQ010000350 DAORUQ010000000
VERSION     DAORUQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96903_length_1920_cov_5.843968"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLW13_16430"
     CDS             <1..579
                     /locus_tag="PLW13_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY72019.1"
                     /translation="YAVLVVNPYYRQQRAPVIPDGATFAEQSTRDVVMPLYQSLDART
                     HVTDAKAFVAWLDAQAPVDSRRKLGTMGYCMGGPITMRTAAALPERIGAGASFHGGGL
                     VTDQPDSPHLLVPRMKARYLIAIAENDDAREPQAKDVLREAFAAAALPAEIEVYAGAM
                     HGWCPPDSAVYNEAQAERAWSRLLAVFGEALA"
     gene            complement(613..1089)
                     /locus_tag="PLW13_16435"
     CDS             complement(613..1089)
                     /locus_tag="PLW13_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQY72020.1"
                     /translation="MKTDMPGKEPIIAKLNQILEHEMAGVVRYTHYSFMVFGFSRIPV
                     VNWFRSAANETLTHAVEAGEMITLAGGYPSLGIGPLLETHQHDLREMLRESMDFEIQG
                     VDLYKELLALAEAAGSVTLEEYARQKIAEEAMHIGDIDKMLRRPGNISQAVKTEAP"
     gene            1254..>1920
                     /locus_tag="PLW13_16440"
     CDS             1254..>1920
                     /locus_tag="PLW13_16440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQY72021.1"
                     /translation="MAIRTRITEMLGIEHPIVQGGMMHVGYAELASAVSNAGGLGIIT
                     ALTQPTPAALSAEIERCRAMTSRPFAVNVTVLPSVTPPDYKAFAQTIIDAGVKIVETA
                     GTQEVRELWAMMKPHGITIMHKCTAVRHALSAERAGCDIISIDGFECAGHPGEDDIPG
                     LILIPAAADRVKLPLLASGGFGDGRGLAAALALGAEGINMGTRFCATQEAPIHTNVKQ
                     KYVE"
BASE COUNT          312 a          634 c          649 g          325 t
ORIGIN      
        1 tatgcggtgc tggtcgtcaa tccctattac cgccagcagc gcgcgccggt gattcccgac
       61 ggcgccacct ttgccgagca gtcgacgcgc gatgtcgtca tgccgctgta ccagtcgctg
      121 gatgcacgca cgcacgtgac cgacgcgaag gccttcgtgg cgtggctcga cgcccaggcc
      181 ccggtggata gcaggcgcaa gctcggcacc atgggctact gcatgggggg gccgatcacg
      241 atgcgcaccg cggccgcgct gcccgaacgc atcggcgctg gcgcctcctt ccacggtggc
      301 ggcctggtca ccgatcagcc cgacagcccg cacctgctgg tgccgcggat gaaggcgcgc
      361 tacctgatcg cgattgccga gaacgacgat gcgcgcgaac cgcaggcgaa agacgtgctg
      421 cgggaggcct tcgccgcggc cgcgctgccg gccgagatcg aggtttacgc cggcgcgatg
      481 cacggctggt gcccgccgga ctccgcggtc tacaacgaag cgcaggcgga gagggcctgg
      541 agccggctgc tggcggtgtt cggggaggcg ttggcctgac gcccgacgtg ccggccgcac
      601 tccaggcggc ccttacggcg cctcggtttt caccgcctgg ctgatgtttc ccggccggcg
      661 caacatcttg tcgatgtcgc cgatgtgcat ggcctcttcg gcgattttct gccgggcgta
      721 ctcttccagc gtgacggagc cggcggcttc ggcgagcgcc aggagttcct tgtacaggtc
      781 gacgccctgg atctcgaaat ccatcgattc gcgcagcatt tcccgcaggt cgtgctggtg
      841 ggtctccagc agcgggccga tgccgagcga tgggtatccg cccgccagcg tgatcatctc
      901 gccggcctcg accgcatgcg tcagcgtctc gttggccgcg ctgcggaacc agttcaccac
      961 gggaatgcgg ctgaagccga agaccatgaa cgagtagtgg gtgtagcgca ccacgcccgc
     1021 catctcgtgc tcgaggatct ggttgagctt ggcgattatc ggttccttgc cgggcatgtc
     1081 ggttttcatc attcacctcc cgttgcctgt tggtgcttgc cccgggtcga cgatactcct
     1141 gcgatggggg cagtggaaca acaaggcccg gttcgaggca tcgcggcggg attcgcatat
     1201 aatccgcggc cgcggacgtc gcgaccacca ggttccagcc cagagaggta aagatggcaa
     1261 tcaggacccg cattaccgaa atgctcggca tcgagcaccc catagtccag ggcggcatga
     1321 tgcacgtggg ctatgccgag ctcgccagcg ccgtctccaa cgcgggtggc ctcggtatca
     1381 tcaccgcgct gacgcagccg actccggccg cgctgagcgc ggagatcgag cgttgtcgcg
     1441 caatgacgtc caggcccttc gcggtcaacg tgaccgtgct gccatccgtc acgccgcccg
     1501 actacaaggc ctttgcgcag acgatcatcg acgccggcgt caagatcgtc gagaccgccg
     1561 gcacgcagga agtgcgcgaa ctgtgggcga tgatgaagcc acacggcatc acgatcatgc
     1621 acaagtgcac cgcggtgcgt cacgcgctct cggccgagcg ggcgggctgc gacatcatct
     1681 cgatcgacgg cttcgagtgc gccggtcatc ccggcgagga cgatattccc ggactgatcc
     1741 tcatcccggc ggcggcggac cgggtgaagc tcccgctgct cgcatcgggt ggattcggtg
     1801 acgggcgcgg gctcgcggcg gcgctggcgc tgggcgcgga ggggatcaac atgggtacgc
     1861 gcttctgcgc cacgcaggag gcaccgatcc acaccaacgt gaagcagaag tatgtcgaga
//
LOCUS       DAORUQ010000351         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_96986_length_1919_cov_4.518777, whole genome shotgun sequence.
ACCESSION   DAORUQ010000351 DAORUQ010000000
VERSION     DAORUQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96986_length_1919_cov_4.518777"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PLW13_16445"
     CDS             complement(<1..1477)
                     /locus_tag="PLW13_16445"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HQY72022.1"
                     /translation="MTTLRLVLGDQLNPCHSWFATPDPDVLHVMMEIREETDYVLHHA
                     QKIIAIFAAMRAFARALRDAGHRVHYFAIDDAGNRQSLCANLDALRVQHRIARFEYQA
                     PDEWRLDRRLADYAAGLPIPARMVDSEHFLGAREEAAALFAGRGQWLMERFYRHMRVR
                     HGVLLDAAGKPAGGQWNFDRDNRKPWRGAPSEPRDRRRTHDHAALWRQIEAAGVKSFG
                     APREHDFRWPLDRAEALAELDAFVAEALPHFGDYQDALARQAWRLFHSLLSFALNVKM
                     LHPREVIARAEAAWREGRVPLAAAEGFIRQVLGWREYVRGVYWAGMPGYAEQNVLGHA
                     LPLPAWFWSGRTRMRCVAHAIGQSLDEAYAHHIQRLMVIGNFALLAGLDPQALHRWYL
                     GVYIDAFEWVELPNTLGMSQFADGGRMATKPYVSSAAYLDRMGDYCKGCPYDRKARTG
                     AMACPFNALYWDFFERHRPRLAGNHRLAMVYVQLGKMAPAEL"
     gene            complement(1474..>1919)
                     /locus_tag="PLW13_16450"
     CDS             complement(1474..>1919)
                     /locus_tag="PLW13_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY72023.1"
                     /translation="AAGARMRACWALMLRYAEPLALPFDAAFVNSGPLRWVARDSSKP
                     GRHAPDTWLLHACAAWSEERLEKAPEAVAAALLEAFAAFGARTPDSWSAHRWRYADTE
                     PAIDRGHVLDAALGIGLCGDWLNGGKVEGAWLSGRALAAAMLAAP"
BASE COUNT          298 a          680 c          681 g          260 t
ORIGIN      
        1 cgagctccgc cggtgccatc ttcccgagct ggacgtagac catggcgaga cggtgattgc
       61 cggcaagccg ggggcggtgg cgttcgaaga aatcccagta caacgcgttg aaggggcagg
      121 ccatggcgcc ggtgcgcgcc ttgcggtcgt acggacagcc cttgcagtaa tcgcccatgc
      181 ggtcgaggta ggccgcgctg gacacgtagg gcttggtcgc catgcggccg ccgtcggcga
      241 actggctcat gccgagcgtg ttcggcaact ccacccactc gaaggcatcg atgtagacgc
      301 cgaggtacca gcgatgcagg gcctgcggat cgagcccggc gagcagcgcg aagttgccga
      361 tcaccatcag gcgctggata tggtgcgcat aggcctcatc gagcgactgg ccgatcgcgt
      421 gcgccacgca gcgcatgcgg gtgcgaccgc tccagaacca cgccggcaag ggcagcgcgt
      481 gccccagcac gttctgctcg gcgtagcccg gcatacccgc ccagtagacg ccgcgcacgt
      541 attcgcgcca gcccaggacc tggcggatga agccctcggc ggccgcgagc ggcaccctgc
      601 cctcgcgcca cgcggcctcg gcacgcgcga tcacctcgcg cggatgcagc atcttcacgt
      661 tcagcgcgaa cgagagcagc gagtggaaca gccgccacgc ctgccgcgcg agcgcgtcct
      721 ggtagtcacc gaaatgcggc agcgcctcgg cgacgaaggc atcgagctcg gcgagcgcct
      781 ctgcacggtc gagcggccag cggaaatcat gttcgcgcgg cgcgccgaaa ctcttgaccc
      841 cggccgcctc gatctgtcgc cacagcgcgg cgtgatcgtg cgtgcggcgg cgatcgcgcg
      901 gctcggacgg cgcgccgcgc cagggcttgc ggttgtcgcg atcaaagttc cactgcccgc
      961 cggccggctt gccggccgcg tccagcagca cgccgtggcg cacgcgcata tggcggtaga
     1021 agcgttccat cagccactgg ccgcgcccgg caaagagcgc cgcggcctcc tcgcgcgcgc
     1081 cgagaaagtg ctcgctgtcg accatgcgcg cgggaatcgg caggcccgcc gcgtagtcgg
     1141 cgagccggcg gtcgagccgc cactcgtcgg gcgcctggta ctcgaagcgg gcgatacgat
     1201 gctgcacgcg cagcgcatcg aggttggcgc acagcgactg ccggttgccg gcatcatcga
     1261 tcgcgaagta gtgcacgcgg tgcccggcat cgcgcaacgc gcgcgcgaag gcgcgcatcg
     1321 cggcgaaaat cgcgatgatc ttctgcgcgt ggtgcagcac gtagtcggtc tcctcgcgga
     1381 tctccatcat gacgtgcagc acgtcgggat ccggcgtggc gaaccagctg tggcaggggt
     1441 tcagctggtc gccgagtacc aggcgcagcg tcgtcatggg gcagccagca tcgccgcggc
     1501 gagcgcgcgc ccgctgagcc aggcgccttc gaccttgccg ccgttcagcc agtcgccgca
     1561 gagcccgata ccgagcgcgg cgtccagcac gtgacctcgg tcgatcgccg gctccgtgtc
     1621 ggcgtaacgc cagcgatgcg cgctccagct gtcgggtgtg cgcgcaccga acgcggcgaa
     1681 ggcctcgagc agcgcggcgg ccaccgcctc cggggccttt tcgagccgct cctcgctcca
     1741 cgcggcacag gcgtgcagca gccacgtgtc cggcgcgtgg cggcccggct tgctgctgtc
     1801 gcgcgccacc cagcgcagcg gcccgctgtt gacgaaggcg gcatcgaagg gcaaggccag
     1861 cggctcggcg tagcgcagca tcagcgccca gcaggcgcgc atgcgggcgc cggccgcga
//
LOCUS       DAORUQ010000352         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_100856_length_1877_cov_5.531833, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000352 DAORUQ010000000
VERSION     DAORUQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100856_length_1877_cov_5.531833"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLW13_16455"
     CDS             <1..290
                     /locus_tag="PLW13_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HQY72024.1"
                     /translation="NELARTGVTIFGLNYKDDSAAARRWIETRGDPYRLNIVDDSGRL
                     GIDLGVTGAPETYLIDAAGIVRLRYQGPLDARVWREQFEPLIARLGAGATP"
     gene            287..754
                     /locus_tag="PLW13_16460"
     CDS             287..754
                     /locus_tag="PLW13_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HQY72025.1"
                     /translation="MRRVLLLLVLLGGPLHAAVETFDFATEEQRQRYLEFAQELRCPK
                     CQNQNLADSNAPIAEDLRLELHRLLLEGKSDAEIVDFMVTRYGEYVLYEPRLEAKTAL
                     LWGAPVLMLGVGLLVLAGIVRRARRAGLARAAALSDAERRRLQELLDEDARQR"
     gene            751..>1877
                     /gene="ccmI"
                     /locus_tag="PLW13_16465"
     CDS             751..>1877
                     /gene="ccmI"
                     /locus_tag="PLW13_16465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HQY72026.1"
                     /translation="MSMFWTAIALLGVLAGLLVLVPLRRAARPAGAGAAADARRAQNL
                     AAYRQQLAELEAARAGGALDDGQFAAARLELDRRVLEDTAAPAGVGARSGSGSGRPAL
                     LACALAVPLLALGLYRHLGATPEIELAALIAQLEQEMPAAQRAELLSRLQPRLEAQVR
                     RADPDGDYRFLLARLYTAEERYPQAAALYAELVDLYPEDAAMIAQSAQALYLAGGRKL
                     TPAVQALVDRAFAIDPAQVTLLGLVGMDRFQAGDYRGALDYWQRLLDHLPPGAPDAGV
                     IRDGIAMARSRLGESAGPAPGAPVPDSGAPPAASGPRLQVAVSLAPGLELRPGDTVFV
                     FARAVGGPPMPLAVARFPATELPRAVELSDAMAMAPGMKLS"
BASE COUNT          244 a          677 c          685 g          271 t
ORIGIN      
        1 tcaacgaact cgcgcgcacg ggcgtgacga tcttcggcct caactacaag gacgactccg
       61 cggcggcgcg gcgctggatc gagacccggg gcgatccgta ccggctcaac atcgtcgacg
      121 acagcggccg gctcggcatc gatctcggtg tgaccggcgc gccggagacc tatctgatcg
      181 acgccgccgg catcgtgcgc ctgcgctacc aggggccgct cgatgcgcgc gtctggcgcg
      241 agcagttcga accgctgatc gcgcggctcg gcgccggggc cacgccatga ggcgcgtgtt
      301 gctgctgctc gtgctgctcg gcggtccgct gcacgccgcc gtggagacct tcgatttcgc
      361 caccgaggag cagcgccagc gctacctcga attcgcgcag gagctgcgct gtcccaagtg
      421 ccagaaccag aacctcgccg attccaacgc gccgatcgcc gaggacctgc gcctcgaact
      481 gcaccggctg ctgctggagg gcaagagcga tgcggagatc gtcgatttca tggtcacgcg
      541 ctacggcgag tacgtgctgt acgaaccgcg gctggaagcg aagaccgcgc tgctgtgggg
      601 cgcgccggtg ctgatgctcg gtgtaggcct gctcgtgctg gcaggcatcg tgcggcgtgc
      661 gcgccgtgcc ggtctcgcgc gcgcggcggc gctcagcgac gccgagcgcc ggcgcctcca
      721 agaactcctg gatgaggacg caaggcagcg atgagcatgt tctggacggc aatcgccctg
      781 ctcggtgtgc tggcgggatt actggtgctg gtgccgctgc gccgtgcggc gcgacccgcg
      841 ggtgcgggcg cggcggccga tgcgcgccgc gcgcaaaacc ttgccgccta ccgccagcag
      901 ctcgcggagc tcgaggccgc tcgcgccggc ggcgcgctcg atgacggaca gtttgccgcc
      961 gcgcgcctgg aactcgaccg ccgcgtgctg gaagacaccg ccgcccccgc aggcgtcggg
     1021 gcgcgcagcg gcagcggcag cggccgcccg gcgctgctcg cgtgcgcgct cgccgtgccg
     1081 ctgctggcgc tggggctgta tcgccacctt ggcgcaaccc ccgagatcga gctggccgcc
     1141 ctgatcgcgc agctcgagca ggaaatgccg gcggcgcagc gcgccgaact cctttcccgg
     1201 ttgcaaccga ggctggaggc gcaggtgcgc cgcgccgatc ccgacggcga ctaccgcttc
     1261 ctgctggcgc gcctctacac cgccgaggaa cgctacccgc aggcggcggc gctctacgcg
     1321 gagctggtcg atctctaccc ggaggatgcg gcaatgatcg cgcagtccgc gcaggcgctg
     1381 tatctcgcgg gcgggcgcaa gctcacgccc gcggtgcagg cactggtcga tcgcgccttc
     1441 gccatcgacc cggcacaggt cacgctgctc ggtctggtcg gcatggaccg cttccaggcc
     1501 ggcgattacc gcggcgcgct cgattactgg cagaggctgc tcgatcacct gccgcccggc
     1561 gcccccgatg ccggcgtgat ccgtgacggc atcgcgatgg cacgctcgcg cctcggcgaa
     1621 agcgccggcc cggcgccggg cgcgccggtc cccgacagcg gtgcgccgcc ggccgcctcc
     1681 ggtccgcgcc tgcaggtggc cgtgagcctc gcgccggggc tggagctgcg ccccggggat
     1741 accgtgttcg tgttcgcgcg cgccgtcggc ggcccgccga tgccgttggc cgtggcgcgt
     1801 tttccggcca cggaactgcc gcgcgccgtg gagctgagcg atgcgatggc catggcgccg
     1861 gggatgaagc tgtcgag
//
LOCUS       DAORUQ010000353         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_101874_length_1866_cov_6.081723, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000353 DAORUQ010000000
VERSION     DAORUQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101874_length_1866_cov_6.081723"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLW13_16470"
     CDS             <1..310
                     /locus_tag="PLW13_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72027.1"
                     /translation="GAVMERPAEIASAIGARELHDGLALWGRIDAPWLAEVGPDRLAA
                     ACRPLRDLEPRLILGSHLPPAAGLDDALYAGLEAAREAAPFVGPDQAALELASSGAYA
                     "
     gene            complement(411..755)
                     /locus_tag="PLW13_16475"
     CDS             complement(411..755)
                     /locus_tag="PLW13_16475"
                     /inference="COORDINATES: protein motif:HMM:NF018637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HQY72028.1"
                     /translation="MTRIQIFESARSEAGRNVRNRLDAMAEIARAREQGANIEHYEFA
                     SDAPEFMRDAQVKAYIDTVGADALPLVLIDGKQALAGRYPTRAELARWSGSSIVKVHP
                     DSGNCCSGGNCG"
     gene            complement(826..1845)
                     /locus_tag="PLW13_16480"
     CDS             complement(826..1845)
                     /locus_tag="PLW13_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HQY72029.1"
                     /translation="MFVTGRIAAHREVLDALDVAALVRRAYAEAGYGEDAARHVWGPR
                     PENLLIDTALCFGEFEPGEREQRHLSDDQAICVGYANTMTDTNHLPPAHWLANRIGRE
                     LFRLRSQRGAGQVGPDGKVLAQVRTDGRAWQPHRVSISLNHHEGSDWLLLRRIAEDAV
                     ARACAGKELPELVLNGAGMFVAGGPNGDNGTTGKKLVIDAYGPTVPIGGGAWSGKDFN
                     KVDRAGGMLARELALIAAKKARSEAWVELRYEPGGAGPAEVGVWIDGVGVSADEAALP
                     AGDALRTATVSRSLIAGVEGPLAERARWGHFTAPRRRRTRLHRPALQPIVRAVGAESA
                     NASSS"
BASE COUNT          242 a          698 c          639 g          287 t
ORIGIN      
        1 cggcgccgtg atggagcgcc ccgccgagat cgcgtcggcg atcggcgcgc gcgaattgca
       61 cgacgggctg gcactgtggg ggcgcatcga cgcgccctgg ctcgccgagg tcgggcccga
      121 ccggctggcg gcggcctgcc gtccgctgcg cgacctggag ccgcgcctga tcctcggcag
      181 tcacctgccg ccggcggcgg gtctcgacga tgcgctgtac gccggcctgg aggccgcgcg
      241 cgaggctgcg ccgttcgtgg gcccggacca ggctgcgttg gagctggctt cgagcggggc
      301 gtatgcgtga tccgcagcgc ccgggctggc ccggtgacgc cttccagagg accccgggcc
      361 gcccgcaacc ggaaatgcgc aacgggctcg aagcggtgcc gtgaagtgct tcatccgcaa
      421 ttgccgccgc tgcagcagtt gccgctgtcg gggtgcacct tgacgatcga cgagccgctc
      481 cagcgcgcca gttcggcgcg cgtgggatag cgtcccgcga gcgcctgctt gccgtcgatc
      541 aggaccagcg gcagcgcatc ggcgcccacg gtgtcgatgt aggccttgac ctgcgcatcg
      601 cgcatgaact cgggagcgtc gctggcgaac tcgtagtgct cgatgtttgc gccctgctcg
      661 cgggcgcgag cgatttccgc catcgcgtcg agacggttcc ggacattgcg tccagcttcc
      721 gaacgtgccg actcgaagat ctggatacgg gtcatcgtgg tactccccgg aacgggcaga
      781 aggggaaatt ctcgcccaac ctgtggcggg gcggaatgat cgcggtcaac tgctggaggc
      841 gtttgctgac tcggcgccga ctgctcgcac gatcggctgc agagcgggac gatgcagtcg
      901 cgtacgacgt cgacgcggtg ccgtgaagtg cccccaccgg gcccgctcgg ccaacggccc
      961 ctcgaccccc gcgatcaagc tccgcgaaac cgtcgcggtt cgcagcgcgt cgccggccgg
     1021 caacgccgcc tcgtccgcgc tcacccccac gccgtcgatc cagaccccca cctccgccgg
     1081 ccccgcgccg cccggttcgt agcgcagctc cacccacgcc tcgctgcgcg ccttcttcgc
     1141 cgcgatcagt gccagctcgc gcgccagcat cccgccggcc cggtccacct tgttgaagtc
     1201 cttgcccgac cacgcaccgc cgccgatcgg caccgtcggc ccgtaggcgt cgatcaccag
     1261 cttcttgccg gtcgtgccgt tgtcgccatt cggcccgccc gcgacgaaca tgccggcgcc
     1321 gttcagcacc agctccggca gttccttccc cgcgcaggcg cgcgcgaccg cgtcctcggc
     1381 gatccgtcgc agcagcagcc agtccgatcc ctcgtggtgg ttcagcgaga tcgacacccg
     1441 atgcggctgc cacgcgcgcc cgtcggtgcg cacctgcgcc agcaccttgc catccggtcc
     1501 gacctgcccc gcgccgcgct gggatctcag ccggaacagt tcgcgcccga tgcggttcgc
     1561 cagccagtgc gcgggcggca ggtggttcgt gtccgtcatt gtgttcgcgt agcccacgca
     1621 gattgcctgg tcgtcggaca ggtgccgttg ctcgcgttcg ccgggctcga actcgccgaa
     1681 gcacagcgcg gtgtcgatga ggaggttctc cggccgcggg ccccacacgt ggcgggcggc
     1741 gtcttccccg tagccggcct cggcgtaggc gcggcgcacc agcgccgcta cgtcgagcgc
     1801 gtcgagcacc tcgcggtgcg cggcgatgcg gccggtgacg aacacgcgct cgaacacgca
     1861 ggcgat
//
LOCUS       DAORUQ010000354         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_102130_length_1863_cov_4.960730, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000354 DAORUQ010000000
VERSION     DAORUQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102130_length_1863_cov_4.960730"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLW13_16485"
     CDS             <1..296
                     /locus_tag="PLW13_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72030.1"
                     /translation="TVSACLDALAQADALLCVGTSLMVYSGFRFCRHAAARGQPVAAL
                     NLGRTRADDLLTLKIAAPCDAVLTALLGELPVQPEVPKALQRSEPPSSTSTMP"
     gene            complement(230..709)
                     /locus_tag="PLW13_16490"
     CDS             complement(230..709)
                     /locus_tag="PLW13_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQY72031.1"
                     /translation="MNGFYQGLAQQLAGLIEDEQDFIANCANCSALLMAGLERLNWAG
                     FYFARDGELVLGPFQGMPACTRIAWGEGVCGTAAQLADCIIVPDVHEFPVHIACDARS
                     RSELVIPLIWQGEVLGVLDLDSPEVDRFGEEDRDGLQGIVEVLLGGSDLWSAFGTSG"
     gene            complement(706..1347)
                     /locus_tag="PLW13_16495"
     CDS             complement(706..1347)
                     /locus_tag="PLW13_16495"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQY72032.1"
                     /translation="MSRLRSFVMLTVLGGLAVILPLVLFVALARWLIGLLANFTAPLA
                     DALGAHLGSGTPLPLLAVVALLVALCFVTGLAVRTRFGALLHDRLDALLGRLAPGYRA
                     VRDTVGQLLGSAERDALRGEVALVAIHGPHSPARQLAIVTARHDDGTLTVYVPTAPVP
                     TQGFVYHLPPECVRLLPEVSVTNAMRMVFACGAGAADILTGAVCGVVQGVSRP"
     gene            1461..>1863
                     /locus_tag="PLW13_16500"
     CDS             1461..>1863
                     /locus_tag="PLW13_16500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQY72033.1"
                     /translation="MPSIFTRIIDGELPGHFVWKDDQAVAFMTIQPIRPGHLLVIPRA
                     EVDHWDALPEATSMHLMRVSQRIARALKDVYSPLRVGLMIAGLEVPHTHLHLLPIDEM
                     GDMSFMKAHAADAASLAAEAQKIRAALRAHGQ"
BASE COUNT          323 a          690 c          597 g          253 t
ORIGIN      
        1 caacggtctc cgcctgcctc gatgcgctcg cacaggccga tgccctgctc tgtgtcggca
       61 cctcgctgat ggtgtactcg ggattccgct tctgccgcca cgccgccgcg cgcggtcaac
      121 cggtcgccgc gctcaacctg ggccgcacgc gcgccgacga cctgctcacc ctgaagattg
      181 ccgcgccctg cgacgcggtg ctgacggcgc tgctcgggga actgccggtt cagccggaag
      241 tgccgaaggc gctccagagg tcggagccgc cgagcagcac ctcgacgatg ccctgaagcc
      301 cgtcacggtc ctcctcgccg aagcggtcga cctccggact gtcgaggtcg agtacgccca
      361 gcacctcgcc ctgccagatc agcgggatca ccagctccga gcgcgagcgc gcatcacagg
      421 cgatgtgcac gggaaactcg tgcacgtccg gcacgatgat gcagtcggcg agctgcgcgg
      481 ccgtgccgca cacgccctcg ccccacgcaa tgcgcgtgca ggcgggcatg ccctggaacg
      541 ggcccagcac cagttcgccg tcgcgcgcga agtagaaacc cgcccagttg aggcgctcga
      601 gcccggccat cagcagcgcg ctgcagttgg cgcaattggc gatgaaatcc tgttcgtcct
      661 cgatcagtcc ggcgagctgc tgcgccaggc cctggtaaaa accgttcacg gcctgctcac
      721 cccttgcacc acgccgcaca cggcaccggt caggatatcc gccgcgcccg cgccgcaggc
      781 gaacaccatg cgcatcgcgt tcgtcaccga cacctccggc agcagccgca cgcactccgg
      841 cggcaggtgg tacacgaagc cctgcgtggg caccggcgcg gtcggcacgt agaccgtcag
      901 cgtgccgtca tcgtggcgcg cggtcacgat ggccagctgg cgcgccggcg agtgtggccc
      961 gtggatggca accagcgcca cttcgccgcg cagcgcgtcg cgctcggccg atcccagcaa
     1021 ttgccccacc gtgtcgcgta ccgcccgata gccgggcgcg agccgcccga gcagcgcgtc
     1081 gagtcgatca tgcagcagcg cgccgaagcg cgtgcgcacc gcgagtccgg taacgaaaca
     1141 cagggccacc agcagcgcca ccaccgcgag cagcggcagt ggcgtgccgc tgcccagatg
     1201 cgcgccgagc gcatcggcca gcggcgcggt gaagttcgcg agcaggccga tgagccagcg
     1261 cgcaagcgcc acgaacagca ccagcggcag gatcaccgcc agcccgccca gcacggtcag
     1321 catcacgaag gaacgcaatc ggctcatcga atcgtccggc agcaacagga acacagcgcg
     1381 cgattgtaac cgcccggcgt ttgtcgcgcg cgcgggcatc accgataatc gggcgcaacc
     1441 aacgcaggag acgatttgcc atgcccagca tttttacccg catcatcgac ggcgagctgc
     1501 ccggccattt cgtgtggaag gacgaccagg ccgtcgcctt catgaccatc cagcccatac
     1561 gccccggcca cctgctggtg atcccgcgtg ccgaggtcga ccactgggac gccctgcctg
     1621 aggccacgag catgcacctg atgcgggtgt cgcaacgcat tgcccgtgcg ctgaaggatg
     1681 tctactcgcc gctgcgcgtg ggcctcatga tcgcgggcct cgaggtgccg cacactcatc
     1741 tgcacctgct gcccatcgac gagatgggcg atatgtcgtt catgaaggcg cacgccgccg
     1801 atgccgcatc gctggcggcc gaggcgcaga agatacgcgc ggccttgcgc gcacacggac
     1861 aac
//
LOCUS       DAORUQ010000355         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_102409_length_1860_cov_5.220499, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000355 DAORUQ010000000
VERSION     DAORUQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102409_length_1860_cov_5.220499"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PLW13_16505"
     CDS             complement(<1..1251)
                     /locus_tag="PLW13_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72034.1"
                     /translation="MSDRHTDWKARYLLALETQERLEHEHEERERELARAVNRISLAC
                     EGQEQELDVQLAGLRDFLRKGRSVSTAVLASKIDVVDERIRLLEERNKLSESAAAAAV
                     DALLAQLALEELPGGLRRRIASLRREAHERLQRTGGIEEILQACAGLQDEVLQALRGQ
                     AAGRPGLLARLFGQPASAAPEALPEVLREAPATGRIGTAADKALDALIDTVEAPDSDS
                     ANRALFDSAKTRIARGVPLDELVTTLDTVRVLAAAALARSRGEFGQFLQQLNERLAAA
                     GAAASGAHVLSQQRAAAGSELSAAIERNIARMRQSADTATEVEQLKREARAHIEQMAS
                     AMTVYRNREDRIQEEFGQRVSALEDRVRELESHSRTAEARVAEQSRIALTDRLSQLPN
                     REAFERRLTEERERRERGGSPLALA"
     gene            complement(1248..>1860)
                     /locus_tag="PLW13_16510"
     CDS             complement(1248..>1860)
                     /locus_tag="PLW13_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY72035.1"
                     /translation="RLREPIEAMKLPAGYSLSWGGEFEDSSKAGAGLKSTLPLGLLAM
                     VLTSVLLFGKLRQPLIIWLTVPLAIIGITAGLLAAGAPFDFMSILGALSLVGLLIKNA
                     IVLIEEIDQQIAAGKPGFEAVVDAAVSRLRPVSLAAATTILGLVPLLGDVFFVNMSIT
                     MMAGLGFATVLTLLVVPALYTLVFGIPVRRGGIRDHTISGTIE"
BASE COUNT          260 a          672 c          636 g          292 t
ORIGIN      
        1 cgccagcgcc agcggcgagc ccccgcgctc gcggcgctcg cgctcttcgg tcagccgtcg
       61 ttcgaatgcc tcgcggttgg gcagctggct caggcggtcg gtcagcgcaa tgcggctctg
      121 ctcggccacg cgcgcctcgg cggtgcgcga gtgtgactcc agctccctga cccggtcttc
      181 cagcgcggaa acccgctggc cgaattcctc ctggatgcgg tcctcgcgat tgcggtagac
      241 ggtcatcgcg ctcgccatct gctcgatgtg ggcacgcgcc tcgcgtttga gctgttccac
      301 ctcggtcgcg gtatcggcgc tctggcgcat gcgggcgatg ttgcgctcga ttgccgcgga
      361 gagttccgag ccggcggcgg cacgctgctg cgagagcacg tgcgccccgc tggcggcggc
      421 gcccgctgcc gcaagacgct cgttcagctg ctgcaggaac tgcccgaact cgccgcggct
      481 gcgcgccagc gctgccgcgg ccaggacccg caccgtgtcg agtgtcgtca ccagttcgtc
      541 gaggggcacg ccgcgcgcga tgcgggtctt tgcggaatcg aacagggcgc ggttcgcgct
      601 gtcgctgtcc ggtgcctcga ccgtatcgat cagggcatcg agcgccttgt cggccgcggt
      661 gccgatgcgg cctgtggcag gagcctcgcg aagcacttcc ggcaatgcct cgggcgccgc
      721 tgacgccggc tgcccgaaca gccgcgcgag caggccggga cgtccggcgg cctggccgcg
      781 cagggcctgc agcacctcgt cctgcaagcc ggcgcaggcc tggagaatct cctcgattcc
      841 cccggtccgt tgcaggcgct cgtgggcctc gcggcgcagc gatgcgatgc gccgcctgag
      901 cccgccgggc agttcctcga gcgccagctg cgcgagcagc gcatcgaccg cggcagccgc
      961 ggcgctctcg ctgagcttgt tgcgctcctc gagcaggcgg atgcgctcgt cgaccacgtc
     1021 gatcttgctc gcgagcacgg cggtggacac ggaccggccc ttgcgcagga aatcgcgcaa
     1081 cccggccagc tgcacgtcga gttcctgctc ctgcccctcg caggccagcg agatgcggtt
     1141 cacggcgcgc gcgagctcgc gctcgcgctc ctcgtgctcg tgctccaggc gttcctgggt
     1201 ctccagcgcg aggaggtagc gcgctttcca gtcggtatgt cgatcgctca ctcgatggtc
     1261 ccggaaatag tgtggtcacg gatgccgccc ctgcgcaccg ggatcccgaa gaccagcgtg
     1321 tagagcgcgg gcaccacgag cagcgtcagc acggtggcga agcccagtcc cgccatcatg
     1381 gtgatcgaca tgttcacgaa gaacacgtcg ccgagcagcg ggaccaggcc gagaatggtg
     1441 gtggcggccg ccagcgacac cgggcgcagc cggctcaccg cagcgtccac gacggcctcg
     1501 aatccgggct tgcccgcggc gatctgctgg tcgatctcct cgatcagcac gatcgcgttc
     1561 ttgatcagca gtcccaccag tgacagcgcg ccgagtatcg acatgaagtc gaacggcgcc
     1621 ccggccgcga gcaggcccgc ggtgatgccg atgatcgcca gcggcaccgt cagccagatg
     1681 atcaggggct ggcgcagctt cccgaacagc agcacgctgg tcagcaccat cgcgagcaag
     1741 cccagcggca gcgtgctctt cagccctgcg ccggccttgc tcgaatcctc gaactcgccg
     1801 ccccacgaca ggctgtaacc ggctggcagc ttcatggcct cgatcggctc gcgcaggcgc
//
LOCUS       DAORUQ010000356         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_103300_length_1851_cov_12.153675, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000356 DAORUQ010000000
VERSION     DAORUQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103300_length_1851_cov_12.153675"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..459)
                     /locus_tag="PLW13_16515"
     CDS             complement(61..459)
                     /locus_tag="PLW13_16515"
                     /inference="COORDINATES: protein motif:HMM:NF014366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72036.1"
                     /translation="MSARRPYVRPMAGWWRRNPWFVEYMVHEATALAVVAYALILLAG
                     LFRLGQGEAAWARWLAFLASPPALALHAVLLLAFAYHAWTWFRIMPRTLPPIRWRGAR
                     VAAATITRIGLAAALVASALLYVLVARLAA"
     gene            complement(456..1211)
                     /locus_tag="PLW13_16520"
     CDS             complement(456..1211)
                     /locus_tag="PLW13_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQY72037.1"
                     /translation="MNASMRTITIDVLRYRPEQESEPVLQRYEVPFTDDMSVLQGLQH
                     IKDELDGTLSFRWSCRMAICGSCGMMINGVPRLACHTFLREFHPGPLRVEALAHFPIE
                     RDLVIVMDDFMAKLERIKPWIIPAQPRALADGEHLQTPAELEAYEQFSACINCLLCYA
                     ACPQYGLDERFIGPAAVALLHRYNLDSRDAGKAQRMPLVHAEEGVFNCTAVGYCSEVC
                     PKHVDPANAVNLNKSESAADYFLRFLAPKGSSR"
     gene            complement(1208..>1851)
                     /locus_tag="PLW13_16525"
     CDS             complement(1208..>1851)
                     /locus_tag="PLW13_16525"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72038.1"
                     /translation="NRLGSNSLAELCVFGKVAGEQAAIHARNAAAPDAAALALHADAA
                     QARALGIVRASGGRERTATLRREMALAMERGCGIYRTGEGMQQACDTLAGLRERYRSL
                     DVEDKSRVFNTDWLLAIELGYQLEVAQAMAVSALHRRESRGAHQRLDGYTARDDAGFL
                     KHTLARYAGADAPVIDYGNVKITRLAPAVRAYGAAAEQSEAARAAAAPQGQAG"
BASE COUNT          275 a          642 c          670 g          264 t
ORIGIN      
        1 gcgcggcgag catgccgcca gcgccgaaca ggacccagaa gatcggggcg ttggaacgcc
       61 tcacgctgcg agcctcgcga ccaggacata cagcagcgcg ctggcgacca gtgccgcggc
      121 caggccgatg cgcgtgatgg tggccgccgc cacgcgcgcg ccgcgccagc ggatcggggg
      181 cagcgtgcgc ggcatgatcc tgaaccaggt ccaggcgtgg tacgcgaacg ccagcagcag
      241 caccgcgtgg agagcgagcg ccggcggact ggcgaggaag gccagccacc gcgcccacgc
      301 cgcctcgccc tggccgagcc ggaacaggcc ggccagcagg atcagcgcat acgccaccac
      361 cgccagtgca gtggcctcgt ggaccatgta ctcgacgaac cacgggttgc ggcgccacca
      421 gcctgccatc ggccgcacgt agggtcgtcg cgcgctcacc gcgagctccc cttgggcgcg
      481 aggaagcgca ggaagtagtc cgcggcgctc tcggatttgt tgaggttgac ggcattggcc
      541 gggtcgacgt gcttggggca gacctcggag cagtagccga ccgcggtgca gttgaacacg
      601 ccctcctcgg catgcaccag cggcatgcgt tgcgccttgc ccgcgtcgcg cgagtcgagg
      661 ttgtagcgat gcagcagggc caccgcggcc ggcccgatga agcgctcgtc gagtccgtac
      721 tgcggacagg ccgcgtagca cagcaggcaa ttgatgcagg cgctgaactg ctcgtatgcc
      781 tcgagctcgg ccggggtctg caggtgctcg ccgtcggcaa gggcgcgcgg ctgcgcggga
      841 atgatccagg gcttgatgcg ctcgagcttg gccatgaaat cgtccatgac gatcacgagg
      901 tcgcgctcga tcgggaagtg cgccagcgcc tcgacccgca gcggtccggg atggaactcg
      961 cgcaggaagg tgtggcaggc caggcgcggc accccgttga tcatcatgcc gcagctgccg
     1021 cagatcgcca tgcggcagga ccagcggaac gagagcgtgc cgtcgagctc gtccttgatg
     1081 tgctgcaggc cctgcagcac cgacatgtcg tcggtgaacg gcacttcgta gcgctgcagc
     1141 accggctcgg actcctgctc cggccggtag cgcagcacgt cgatggtgat ggtgcgcatg
     1201 ctggcgttca tccggcttgc ccttgcggcg cggccgcggc cctggccgcc tcggactgtt
     1261 ccgcggcagc gccgtaggcg cgcacggcgg gtgccaggcg ggtgatcttc acgttgccat
     1321 agtcgatcac gggggcatcc gcgcccgcgt agcgggccag cgtgtgcttg aggaaaccgg
     1381 cgtcgtcgcg cgcggtgtag ccgtccaggc gctggtgcgc gccgcgcgac tcgcgccggt
     1441 gcagcgcgga caccgccatc gcctgcgcca cctcgagctg gtagcccagc tcgatcgcga
     1501 gcagccagtc ggtgttgaac acccgcgact tgtcctcgac gtcgaggctg cggtaacgct
     1561 cgcgcaggcc ggcgagggtg tcgcaggcct gctgcatgcc ttcgccggtg cgatagatgc
     1621 cgcagccgcg ctccatggcc agcgccattt cgcgccgcag ggtcgcggtc cgttcgcggc
     1681 cgccgctggc ccgcacgata ccgagcgcgc gcgcctgcgc ggcatcggca tgcagcgcca
     1741 gcgcggcggc atccggtgcc gcggcgttgc gcgcgtggat ggccgcctgc tcgcccgcga
     1801 ccttgccgaa cacgcacagt tccgccagcg agttcgatcc cagccggttg g
//
LOCUS       DAORUQ010000357         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_104165_length_1842_cov_6.029659, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000357 DAORUQ010000000
VERSION     DAORUQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104165_length_1842_cov_6.029659"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLW13_16530"
     CDS             complement(<1..897)
                     /locus_tag="PLW13_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HQY72039.1"
                     /translation="MSRAGMLQQLRVLARGSFGIPLLLLTVLAMMTLPIPPLLLDALF
                     SFNIALSLVVLLVAVYALRPLDFAVFPTILLVTTLLRLALNVASTRVVLLEGHTGTDA
                     AGKVIEAFGTVVIGGNYVVGLVVFLILIIINFVVVTKGAGRISEVSARFTLDAMPGKQ
                     MAIDADLNAGLINQDDARRRREEVAQEADFYGAMDGASKFVRGDAVAGVLILAINLIG
                     GLAIGVLQHDLGFADAMQLYSLLTIGDELENNPTAITARPSVENFAKPAAKPAPQAAP
                     KPAPASVQPAAKPAPAPAPKVAP"
     gene            complement(1043..>1842)
                     /locus_tag="PLW13_16535"
     CDS             complement(1043..>1842)
                     /locus_tag="PLW13_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HQY72040.1"
                     /translation="SALAPKFERLDPVRGLQRMFSVRSLVELLKAIAKVLLVGTITVL
                     LLWTLRGELAAMAMQPLEPAIHHAVTMLGWSGLALSASTLLIAALDVPFQLFDHAQKL
                     RMTMQQVRDELKESEGRPEVKSRIRQLQRQFARSRMMAAVPKADVVITNPTHYAVALR
                     YDAGRAAAPMLLAKGSDLVALKIREIAEAHGVPVVSSPRLARAIWFTTEIEAEIPAGL
                     YLAVAQVLAYVFHLRNYRRGRGSAPVLPDELPIPEGMDIPGGAARAG"
BASE COUNT          309 a          624 c          634 g          275 t
ORIGIN      
        1 gggagcgacc ttcggagcgg gggccggagc gggtttggcc gcaggctgta cagacgcagg
       61 ggcaggtttg ggggcggctt ggggagcggg tttggccgca ggtttggcaa aattttcgac
      121 cgaaggacgg gcggtaatgg cggttggatt gttttcgagt tcgtcgccga tcgtgagcag
      181 ggagtagagc tgcatggcat cggcgaagcc gagatcgtgc tgcagcacgc cgatcgccaa
      241 ccccccgatc aggttgatcg cgaggatcag cacgccggct accgcatcgc cgcgcacgaa
      301 cttgctggca ccgtccatgg cgccgtagaa atcggcctcc tgcgcgacct cctcgcggcg
      361 ccggcgcgcg tcgtcctggt tgatcagccc ggcgttcagg tcggcatcga tcgccatctg
      421 cttgcccggc atcgcgtcca gggtgaaacg cgcgctgacc tccgagatgc gcccggcgcc
      481 cttggtgacg accacgaagt tgatgatgat caggatcagg aacaccacca ggccgacgac
      541 gtagttgccg ccgatcacga ccgtgccgaa ggcctcgatc accttgcccg ccgcatcggt
      601 cccggtgtgg ccctcgagca gcacgacgcg ggtcgaggcc acgttgagcg ccaggcgcag
      661 cagcgtcgtc accagcagga tggtcggaaa caccgcgaag tccagcgggc gcagcgcgta
      721 gaccgccacc agcagtacca ccagcgacag cgcgatattg aacgagaaca gcgcgtccag
      781 cagcagcggc ggtatcggca gcgtcatcat cgcgagcacg gtgagcagca gcaacggaat
      841 cccgaagctg ccccgcgcga gcacgcgcag ctgctgcagc atgcccgcgc gactcatgcg
      901 cgcgctgccg attgccatct cagccgcggt cgggccggct gcgccggccg gtgtcgctgt
      961 tcaaattctt gacgctcctg cgctgtgata cccctttcag tgcgagcaca gcaagaatcg
     1021 ctccagctgt gcccgtaccg ggctacccgg cgcgcgccgc gccgcccggg atgtccatgc
     1081 cctcggggat gggcaactcg tccggcagca cgggcgcgga tccgcgaccg cgacggtaat
     1141 tgcgcaggtg gaacacgtag gcgagcacct gcgcgacggc gaggtagagg cccgccggga
     1201 tctcggcctc gatctcggtg gtgaaccaga tcgcgcgcgc tagacgcggc gagctcacca
     1261 cgggcacccc gtgggcctcg gcgatctcgc ggatcttcag cgcgaccagg tcgctgccct
     1321 tggccagcag catgggcgcg gcggcgcgac cggcgtcgta gcgcagggcc accgcgtagt
     1381 gcgtggggtt ggtgatcacg acatcggcct tcggcacggc cgccatcatg cggctgcgcg
     1441 cgaactggcg ctgcaactgg cgaatgcggc tcttgacctc ggggcggccc tcggactcct
     1501 tgagctcgtc gcgcacctgc tgcatggtca tgcgcagctt ctgcgcatgg tcgaacagct
     1561 ggaacgggac gtcgagcgcc gcgatgagca gcgtcgaggc gctgagcgcg agccccgacc
     1621 agcccagcat cgtcacggca tggtggatcg ccggttccag cggctgcatc gccatcgccg
     1681 cgagctcgcc gcgcagcgtc cacagcagca gcaccgtgat cgtccccacc agcagcacct
     1741 tggcgatcgc cttgagcaac tcgacgaggg agcgcaccga gaacatccgc tgcaggcccc
     1801 gcaccggatc caggcgctcg aacttcggtg ccagcgcgct cg
//
LOCUS       DAORUQ010000358         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_104763_length_1836_cov_6.084222, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000358 DAORUQ010000000
VERSION     DAORUQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104763_length_1836_cov_6.084222"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1836
                     /locus_tag="PLW13_16540"
     CDS             <1..>1836
                     /locus_tag="PLW13_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY72041.1"
                     /translation="LAGRTVHLLPDDLGVEALSGAFQDAANNYSLVKITPAHLQVLQL
                     ALSADQAAKRTRVFVIGGEQLEAEAVEFWRRHAADTVLVNEYGPTEATVGCCTYSVTP
                     GTRSNGAIPIGRPVANTQLYILDEYGEPVPIGVIGELYIGGEGVARGYHRRPELTAEK
                     FVPDPFSGVPSARLYRTGDLVRYLPDGNIEFIGRRDHQVKLRGYRIELGEIEAVLYQH
                     PAVREVVVLAREDVRGDKRLVAYLVPRQAAPPAAELKEHLVRTVPQHMVPSAFVFLQA
                     LPLTPNGKVDREALPAPERIVGAEGNYVPARTAAEEIIAGIWAEVLGLERVGVEDNFF
                     ELGGHSLLAVSVIERMRRAGLHAAVRTLFMSPTVADMAAAAGGESGVVEVPPNRIAPG
                     CEVIAPQMLPLVRLAPGEIERIVEEVPGGAVNVQDIYPLAPLQEGILFHHLMAGEGDP
                     YLLHSLAAFDTRARLDGYLRALRSVIGRHDILRTAVLWEGLPEPVQVVWREAPLVVEE
                     VSLDGAAGDVAQQLAARFDPRRYRLDVRKAPLLRVYIAHDCGSARWVMLLLYHHLTVD
                     HTTAEVIQQEIQAHLLDRAGELPAPLPFRNFVAQARLGIAREEHE"
BASE COUNT          288 a          578 c          667 g          303 t
ORIGIN      
        1 tgctcgcggg ccgcacggtt catctcctgc cggacgacct gggcgtcgag gcgctttccg
       61 gcgcgtttca ggatgccgcc aacaactaca gcctggtcaa gataactccc gcgcacttgc
      121 aggtcctgca gcttgcgctg tcggcagacc aggcagcgaa gagaaccagg gtgttcgtca
      181 tcggcgggga gcaactcgaa gccgaggctg tcgagttctg gcgccggcat gcggctgaca
      241 ccgtgctcgt caacgagtac ggtccgacgg aagcgacagt gggatgctgc acgtattcgg
      301 tgacgccagg gactcgatcc aatggagcga tccccattgg ccgaccggta gccaatacgc
      361 aactctacat tctcgacgaa tacggggaac cggtcccgat cggggtcatc ggggagcttt
      421 acatcggcgg ggagggggtc gcgcggggct atcaccggcg gcctgagctc acggcggaga
      481 agttcgttcc cgatccattc agcggcgtcc cctcggcgcg gctctatcga accggggacc
      541 tggtgcggta tctccccgac ggcaacatcg agttcatcgg ccggcgcgac catcaggtga
      601 agcttcgcgg ctaccgcatc gagctgggcg agatcgaggc ggtcctgtac cagcatcccg
      661 ccgtccggga agtggtggtg ctggcgcgcg aggatgtgcg cggggacaag cgactggtag
      721 cctacctggt tccgaggcag gcggcgcccc cggccgcgga gctgaaggaa cacctcgtac
      781 ggacggtgcc gcaacacatg gtgccgagcg ccttcgtgtt cttgcaggcg ttgccgctga
      841 cccccaacgg caaggtggac cgggaggcgt tgccggcgcc cgagcgcatt gtgggcgcgg
      901 aggggaacta cgtccctgcg cgcacggccg ccgaggagat catcgccggg atctgggcgg
      961 aggtgctggg gctcgagcgc gtcggcgtgg aggacaactt cttcgagctc ggcgggcatt
     1021 cgctgctcgc ggtgagcgtg atcgagcgca tgcgccgcgc ggggcttcat gcagcggtac
     1081 gcacgctctt catgtcgccc acggtggccg acatggcggc cgcggcgggc ggcgagagcg
     1141 gcgtggttga agtgccgccc aaccggattg caccggggtg cgaggtgatt gccccgcaaa
     1201 tgctcccgct ggttcgactc gcgcccgggg agatcgagcg catcgtcgag gaggtgcccg
     1261 gtggtgcggt caatgtccag gacatctatc cgctcgcgcc actacaggaa gggatccttt
     1321 tccaccactt gatggcggga gagggtgacc cctacctgct ccattccctg gccgccttcg
     1381 acacccgcgc ccggctggac gggtacttgc gggccctgcg gtcggtgatc gggcgccacg
     1441 acatcctgcg cacggcggtg ctgtgggagg ggctgcccga gccggtgcag gtcgtgtggc
     1501 gcgaggcgcc tctcgtggtg gaggaagtca gcctcgatgg tgccgcgggc gatgtggcgc
     1561 agcaactcgc ggcacggttc gacccgcgcc gctaccggct cgacgtgcgc aaggcgccgc
     1621 tgctgcgggt gtacatcgcg catgattgcg ggagcgcacg ctgggtgatg ctgctcctgt
     1681 atcaccacct gacggttgac cacaccacgg cggaagtcat acagcaggag atccaggcgc
     1741 acctgctgga tcgggccggg gagctgcccg cgccgttgcc ctttcgcaac ttcgtggcgc
     1801 aggcgcggct gggcattgcg cgcgaggaac acgagg
//
LOCUS       DAORUQ010000359         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_105841_length_1825_cov_6.251977, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000359 DAORUQ010000000
VERSION     DAORUQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105841_length_1825_cov_6.251977"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="bcrB"
                     /locus_tag="PLW13_16545"
     CDS             <1..982
                     /gene="bcrB"
                     /locus_tag="PLW13_16545"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HQY72042.1"
                     /translation="DLLLLSYTGCFTFMKWFELVRHKYGCETVMLHVPYQGEGKIAPN
                     MRDYVVKQLKETVIPTLERVSGVKFDIDRLRQYMKESVKAEDDLVRVLQSAKNRPSPI
                     DGYFGAVYYIGPIFTAFRGTPEATEYYRVLREEIEERVRLGLGPVTPEGNMGEEKYRL
                     VVEGPPNWTSFRDFWKMFYDEGAVVVSSTYSKVGGLYDFGFRHDPDNPLESLAEYCLG
                     CYTNLNLPSRVDMICKYIEEYQADGLLINSIKSCNSFSAGQLLILREVEKRTGKPAAF
                     IETDLVDPRYFSAANVKNRLESYFQMVKQKRTYGHPDPGNLARAIPIHAQ"
     gene            1012..>1825
                     /locus_tag="PLW13_16550"
     CDS             1012..>1825
                     /locus_tag="PLW13_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQY72043.1"
                     /translation="MRCFIGIDLGSTTTKAVVIDENQNILGRGITNSRSNYDTAAAVA
                     KTEALINGRFYLFRQALQASGALDHELDGFIAQLERDFRSEQYLEQLVDLEATCRRNL
                     TISNFESVVGDALDEVFKRLSAEAPSLFAPGAKRKSDFFRDIAGSQYLAVGEEVAKAL
                     GTRYDFLLNLFDRSIIEVENRVYGDSVRRHLLSALERSFVALPQMQGRRAEIESPIKG
                     ILDTEMEETYTVGTGYGRARLPFSKEHIRSEILCHGLGAHVMYPGTATVLDIG"
BASE COUNT          342 a          634 c          569 g          280 t
ORIGIN      
        1 cgacctgctg ctgctgagct acaccggctg cttcaccttc atgaagtggt tcgagctggt
       61 gcgccacaag tacggctgcg agacggtgat gctgcacgtg ccctaccagg gcgagggcaa
      121 gatcgcgccg aacatgcgcg actacgtcgt caagcagctc aaggagaccg tcatcccgac
      181 gctcgagcgc gtctcgggcg tcaagttcga catcgaccgc ctgcgccagt acatgaagga
      241 atcggtcaag gccgaggacg acctggtgcg cgtgctgcag tcggccaaga accggcccag
      301 cccgatcgac ggctacttcg gcgcggtcta ctacatcggc cccatcttca ccgccttccg
      361 cggcaccccc gaggccaccg agtactaccg tgtgctgcgc gaggagatcg aggagcgcgt
      421 gcgcctgggg ctgggccccg tcacgcccga gggcaacatg ggcgaggaga agtaccgcct
      481 ggtggtcgag ggcccgccga actggaccag cttccgcgac ttctggaaga tgttctacga
      541 cgagggcgcg gtggtggtca gctcgaccta ttccaaggtc ggcggcctgt acgacttcgg
      601 cttccgccac gaccccgaca acccgctgga gtcgctggcc gagtactgcc tgggctgcta
      661 caccaacctg aacctgccct cgcgcgtgga catgatctgc aagtacatcg aggagtacca
      721 ggccgatggc ctgctgatca actcgatcaa gagctgcaac agcttctcgg ccggccagct
      781 gctgatcctg cgcgaggtgg agaagcgcac cggcaagccg gccgccttca tcgagaccga
      841 cctggtcgac ccgcgctact tctcggcggc caacgtcaag aaccggctgg agagctactt
      901 ccagatggtc aagcagaagc gcacctacgg ccaccccgac ccggggaatc tcgcccgcgc
      961 gatcccgatc cacgcccaat aaggccgatt ctccagcaaa ggacccacac catgcgttgc
     1021 ttcatcggca tcgacctcgg ctcgaccacc accaaggcgg tggtgatcga cgagaaccag
     1081 aacatcctgg gccgcggcat caccaactcg cgctcgaact acgacaccgc cgcggcggtg
     1141 gccaagaccg aggcgctcat caacgggcgc ttctacctgt tccgccaggc gctgcaggcc
     1201 agcggcgcgc tggaccacga gctggacggc ttcatcgccc agctggagcg cgacttccgc
     1261 tccgagcagt acctggagca gctggtcgac ctggaggcca cctgccggcg caacctgacg
     1321 atctcgaact tcgagagcgt cgtgggcgat gcgctcgatg aggtcttcaa gcgcctgagc
     1381 gccgaggcac cgtcgctgtt cgcgcccggc gccaagcgca agagcgactt cttccgcgac
     1441 atcgccggca gccagtacct ggccgtcggc gaggaggtgg ccaaggcgct gggcacacgc
     1501 tacgacttcc tgctcaacct gttcgaccgc tcgatcatcg aggtcgagaa ccgcgtctac
     1561 ggcgactcgg tgcgccgcca cctgctgtcc gcgctggagc gcagctttgt cgcgctgccg
     1621 cagatgcagg gccggcgtgc cgagatcgag tcaccgatca agggcatcct cgacacggag
     1681 atggaagaga cctacaccgt cggcaccggc tacggccgcg cgcgactgcc cttcagcaag
     1741 gaacacatcc gcagcgagat tctctgccac ggcctgggcg cgcacgtgat gtacccgggc
     1801 accgccaccg tgctcgacat cggcg
//
LOCUS       DAORUQ010000360         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_109001_length_1794_cov_5.843588, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000360 DAORUQ010000000
VERSION     DAORUQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109001_length_1794_cov_5.843588"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLW13_16555"
     CDS             complement(<1..701)
                     /locus_tag="PLW13_16555"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY72044.1"
                     /translation="MILLHGLFGLSANLGPMARELARDYRVLLPDLRNHGRSFHAPGM
                     GYALMAQDVLRLMDAHGIATAALVGHSMGGKVAMQAALDNPGRIERVLVGDIAPVDYA
                     AHHQRVFAALREAAADSLADRRATQRILERHIAEPGVVAFMLMNRAQHEDRSWHWRFD
                     LGEIEAGYPEILAAPRGAAPYEGPVLFIKGELSDYILPSANEATRALFPRARLKVIAG
                     AGHWLHAEKPAIFLRL"
     gene            863..1615
                     /locus_tag="PLW13_16560"
     CDS             863..1615
                     /locus_tag="PLW13_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72045.1"
                     /translation="MRALLLILTTLCLVACASSPGGDSGGGTAYQATYDARLADKAQF
                     GRVVIAPVNFGKPSRNYLAEHEARIDRLVAQRLTAAGYEVLPSELFEKAWKEGVRNWG
                     EPYNPGTGKLNDTVFQYVLSETVKALAADGRVQAIVFTNLEELQVYFSPSGNHQAHFL
                     GVTRRPSSRGGDGVAADFDWVQGVDAVGLYINVFDLQLQRLYNGAGGIEVTEYLNLKP
                     STPRWTREKTVLDNESFINEGLDLALQPWLQP"
BASE COUNT          289 a          641 c          594 g          270 t
ORIGIN      
        1 agacgcagga agatcgcggg cttctccgcg tgcagccagt gccccgcgcc cgcgatgacc
       61 ttgagccggg cccgcgggaa cagcgcccgc gtggcctcgt tcgccgacgg caggatgtag
      121 tcgctgagct cgcccttgat gaagagcacc ggcccctcgt agggcgcggc gccccgcggc
      181 gccgcgagga tctcgggata gcccgcctcg atctcaccga ggtcaaagcg ccagtgccag
      241 ctgcggtcct cgtgctgcgc gcggttcatc agcatgaacg ccaccacgcc gggctcggcg
      301 atgtggcgct cgaggatgcg ctgggtcgcg cgacggtccg cgagcgaatc ggccgccgcc
      361 tcgcgcagcg ccgcgaacac gcgctggtgg tgcgccgcgt aatcgaccgg cgcaatgtca
      421 ccgaccagca cgcgctcgat gcgcccggga ttgtcgagcg cggcctgcat cgccaccttg
      481 ccccccatcg agtgcccgac cagggccgcc gtcgcgatcc cgtgcgcgtc catcaggcgc
      541 agcacgtcct gcgccatcag cgcatagccc atcccgggcg cgtggaacga gcggccatgg
      601 ttgcgcaggt ccggcagcag cacgcggtag tcgcgcgcca gctcgcgggc catcgggccg
      661 aggttcgccg acaagccgaa caggccgtgc agcaggatca ctgcgcctcc cgcgcttccg
      721 tggatctggc aatggagcag ttcggtcacc ctggcgtgcg tccggcgtgg catgcgggat
      781 cccgatggga gcacggcgcg gttctcattg gcaatagcgg ttgctagaat ccgcgcgcaa
      841 cccgttccac aaggtatgcc cgatgcgcgc gctcctgctg atcctcacga ccctgtgcct
      901 cgtcgcctgc gccagctcgc ctggcggcga cagcggcggc ggcacagcct accaggcgac
      961 ctacgacgcg cgcctcgcgg acaaggcgca gttcggccgc gtggtgatcg ccccggtcaa
     1021 cttcggcaag ccgagccgca actacctcgc cgagcacgag gcgcgcatcg accgcctggt
     1081 ggcgcagcgc ctgacggcgg caggctacga agtgctgcct tcggagctgt tcgagaaggc
     1141 gtggaaggaa ggcgtgcgca actggggcga gccctacaac cccggcaccg gcaagctgaa
     1201 cgacaccgtg ttccagtacg tgctcagcga gaccgtcaag gcgctggctg ccgacggccg
     1261 ggtgcaggcc atcgtgttca ccaacctcga ggaattgcag gtctatttct ccccgagcgg
     1321 caaccaccag gcgcatttcc tcggcgtgac ccgccgccct tccagtcgcg gcggcgacgg
     1381 cgtggcggcg gacttcgact gggtgcaggg cgtggacgcc gtgggcctct acatcaacgt
     1441 cttcgacctg caactgcagc gtctctacaa cggcgccggc ggcatcgaag tcacggagta
     1501 cctgaacctc aagccctcca ccccgcgctg gacgcgcgag aagacggtgc tggacaacga
     1561 gagcttcatc aacgaaggtc tggacctcgc gctgcagccc tggctgcaac cgtaactccg
     1621 gattcatccg gacgggtgct tttcgtgaac ttgcggttct cccccgcctg tgccccgctg
     1681 actctcatgc tcggacacgc cgtgaatacg tccatgtagg ctcgactgtc gccatccagg
     1741 cgacagacgg tcctcgcacg acagccagcg gggcacaggc tgcgctgctg gcac
//
LOCUS       DAORUQ010000361         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_111603_length_1769_cov_12.729288, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000361 DAORUQ010000000
VERSION     DAORUQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111603_length_1769_cov_12.729288"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLW13_16565"
     CDS             <1..185
                     /locus_tag="PLW13_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72046.1"
                     /translation="RDDAGFLKHTLATYAGADAPVIDHGAVKITRLAPAVRAYGAAAE
                     QSEAAQAAASPRGHAG"
     gene            182..937
                     /locus_tag="PLW13_16570"
     CDS             182..937
                     /locus_tag="PLW13_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQY72047.1"
                     /translation="MNASPRNITIEVLRYRPEQESEPVLQRYEVPFTDDMSVLQGLQH
                     IKDELDGTLSFRWSCRMAICGSCGMMINGVPRLACHTFLREFHPGPLRVEALAHFPIE
                     RDLVIVMDDFMAKLERIKPWIIPAQPRALADGEHLQTPAELEAYEQFSACINCLLCYA
                     ACPQYGLDERFIGPAAVALLHRYNLDSRDAGKAQRMPLVHAEEGVFNCTAVGYCSEVC
                     PKHVDPANAVNLNKSESAADYFLRFLAPKGSSR"
     gene            934..1332
                     /locus_tag="PLW13_16575"
     CDS             934..1332
                     /locus_tag="PLW13_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72048.1"
                     /translation="MSARRPYVRPMAGWWRRNPWFGEYMLHEATALAVAAYALILLAG
                     LFQLGRGEAAWVQWLAFLASPLALALHALLLLAFAYHAWTWFKIMPRTLPPIRFRGAR
                     VAAGTITRIGLGAALVASALLYAVVARLAA"
     gene            1329..1682
                     /gene="frdD"
                     /locus_tag="PLW13_16580"
     CDS             1329..1682
                     /gene="frdD"
                     /locus_tag="PLW13_16580"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF014378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HQY72049.1"
                     /translation="MKRSNAPIFWTLFGAGGMLAALVGAMLVYLTGIAGPARIGLPAA
                     TMDYAQVLAFARHPASQLVLFGVIALFLWHAAHRIYHSLHDLGVHVGLAAKLACYGSA
                     MAGTLATVVALWRLG"
BASE COUNT          243 a          645 c          602 g          279 t
ORIGIN      
        1 cgcgcgacga cgccggcttc ctcaagcaca cgctggccac ctacgccggc gcggacgcgc
       61 ccgtgatcga ccatggcgcg gtgaagatca cccgcctggc ccccgccgtg cgcgcctatg
      121 gcgcagccgc ggagcagtcc gaggcggcgc aggccgcagc cagcccgcgg gggcacgccg
      181 gatgaacgcc agtccgcgca acatcaccat cgaagtgctg cgctaccggc cggagcagga
      241 gtccgaaccg gtgctgcagc gctacgaagt gccgttcacc gacgacatgt cggtgctgca
      301 gggcctgcag cacatcaagg acgagctcga cggcacgctc tcgttccgct ggtcctgccg
      361 catggcgatc tgcggcagct gcggcatgat gatcaacggg gtgccgcgcc tggcctgcca
      421 caccttcctg cgcgagttcc atcccggacc gctgcgggtc gaggcgctgg cgcacttccc
      481 gatcgagcgc gacctcgtga tcgtcatgga cgatttcatg gccaagctcg agcgcatcaa
      541 gccctggatc attcccgcgc agccgcgcgc ccttgccgac ggcgagcacc tgcagacccc
      601 ggccgagctc gaggcatacg agcagttcag cgcctgcatc aattgcctgc tgtgctacgc
      661 ggcctgtccg cagtacggac tcgacgagcg cttcatcggg ccggccgcgg tggccctgct
      721 gcatcgctac aacctcgact cgcgcgacgc gggcaaggcg caacgcatgc cgctggtgca
      781 tgccgaggag ggcgtgttca actgcaccgc ggtcggctac tgctccgagg tctgccccaa
      841 gcacgtcgac ccggccaatg ccgtcaacct caacaaatcc gagagcgccg cggactactt
      901 cctgcgcttc ctcgcgccca aggggagctc gcgatgagcg cgcggcggcc ctacgtgcgg
      961 ccgatggcgg gctggtggcg ccgcaacccg tggttcggcg agtacatgtt gcacgaggcc
     1021 accgcgctgg cggtggcggc gtatgcgctg atcctgctgg ccggcctgtt ccagctcggc
     1081 cggggcgagg cggcgtgggt gcagtggctg gccttcctcg ccagtccgct cgcgctggcg
     1141 ctgcacgcgc tgctgctgct ggccttcgcg taccacgcct ggacctggtt caagatcatg
     1201 ccgcgcacgc tgccgccgat ccgcttccgc ggcgcgcgcg tggcggcggg caccatcacc
     1261 cgcatcgggc tgggcgcggc gctggtcgcc agtgcgctgc tgtatgccgt ggtcgcgagg
     1321 ctcgcggcgt gaagcgttcc aacgccccga tcttctggac cctgttcggc gctggcggca
     1381 tgctcgccgc gctggtcggc gcgatgctgg tgtatctcac cggcatcgcc gggccggcgc
     1441 ggatcggctt gccggcagcg accatggact acgcccaggt gctcgccttc gcgcggcacc
     1501 cggcgagcca actggtcctg ttcggcgtga tcgctttgtt cctgtggcac gccgcgcacc
     1561 gcatctacca cagcctccac gacctcggcg tccacgtcgg cctggctgcg aagctggcct
     1621 gctacggcag cgccatggcc ggcaccctcg cgaccgtcgt cgcgctgtgg cggttgggct
     1681 gacgcgcggc gcctcatcgc gccctagtag aggaacggga acacgacctt gcgctgcggc
     1741 gggtagtccg cgaagcgcgt ccggtacca
//
LOCUS       DAORUQ010000362         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_111617_length_1769_cov_4.330222, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000362 DAORUQ010000000
VERSION     DAORUQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111617_length_1769_cov_4.330222"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PLW13_16585"
     CDS             complement(<1..1636)
                     /locus_tag="PLW13_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQY72050.1"
                     /translation="MRIHELPVDAALASLKSGVDGLSASEAARRLAEFGPNRLEDVAR
                     ERLWLRLAREFTHFFAIILWLAAALAFTADWLQPGEGMGRLGVAILCVILVNGLFSFW
                     QEYKAERAVAALRQLLPLRVKALRAGAIVQLPAAELVPGDVVLLEEGDSVPADCRVIE
                     AFGLRVNTATVTGESLPKARSPEPNGEESLLFARNIVLAGTAVASGQARAVVYATGMH
                     TEFGRIAHLTQVAGDTGSPLQREIARLSRIVAMLACGVGIAFFLIGQALGLPFWENFL
                     FAIGIIVANVPEGLAPTVTLSLAMATQRMARRNALVRHLPAVETLGSTTVICCDKTGT
                     LTQNRMAVRRLWLAGEECGADELPERPELQRLGQQLFSNAALCHDLKETAGEQGIELV
                     GDPMEMALVACGRRFAGSLDAYTRLDEIPFDTDRKRMSVLCDSPRGRVLYCKGALERV
                     LTVCDRVQTADGVVALDEAGRARLLAAQDAMAGQGLRVLAFAHREVRNVGECPAEAGM
                     VLAGLVGLEDPPRAQVPAAIARCVEAGIRVIMITGDH"
BASE COUNT          283 a          654 c          591 g          241 t
ORIGIN      
        1 gatgatcgcc ggtgatcatg atcacgcgga tgccggcctc gacgcagcgc gcgatggccg
       61 ccggcacctg cgcgcgcggc ggatcctcga gaccgaccag cccggcgagc accattccgg
      121 cttcagccgg acattcgcca acattccgca cctcccggtg cgcgaacgcc agcacgcgca
      181 atccctgccc ggccatcgcg tcctgcgcgg ccagcaggcg cgcgcgcccg gcctcgtcca
      241 gcgcgaccac gccgtcggcg gtctgcacgc ggtcgcagac cgtgagcacg cgctccagcg
      301 cacccttgca gtacagcacc cgtccgcgcg gcgaatcgca cagcaccgac atgcgcttgc
      361 ggtcggtatc gaagggaatc tcgtcgagcc gcgtatacgc gtcgaggctg cccgcgaaac
      421 gccgcccgca ggcaacgagc gccatttcca tcgggtcgcc gaccagctcg atgccctgct
      481 cgccggcagt ctccttgagg tcgtggcaga gcgccgcgtt gctgaagagc tgctgcccga
      541 ggcgctgcag ctcgggccgc tcgggcagtt cgtccgcgcc gcactcctcg ccggccagcc
      601 acagccgccg caccgccatg cggttctgtg tcagcgtgcc ggtcttgtcg cagcagatca
      661 cggtggtgga accgagcgtc tccaccgcgg gcaggtggcg caccagggcg ttgcgccgcg
      721 ccatgcgctg tgttgccatc gcgagcgaga gcgtcacggt gggcgcgagc ccttccggca
      781 cgttcgccac gatgatgcca atcgcgaaca ggaagttctc ccagaacggc agccccagcg
      841 cctggccgat gaggaagaac gcgatgccca cgccgcaggc gagcatcgcc acgatgcgcg
      901 acaagcgcgc gatctcgcgc tgcagcggcg agccggtgtc gccggcaacc tgcgtcaggt
      961 gcgcgatgcg cccgaactcg gtgtgcatgc cggtggcgta gaccacggca cgcgcctgcc
     1021 ccgacgccac cgcggtaccg gccagcacga tgttgcgcgc gaacagcagc gactcctcgc
     1081 cgttgggttc cggagagcgg gctttcggca gcgattcccc ggtgacggtc gcggtgttca
     1141 cgcgcagccc gaaagcctcg atcacgcggc agtcggccgg cacgctgtcg ccctcctcca
     1201 gcagcaccac gtcgcccggc accagctcgg ccgcgggcaa ctgcacgatg gcgccggcgc
     1261 gcagcgcctt cacccgtagc ggcagcagct ggcgcaaggc cgccacggcg cgctcggcct
     1321 tgtactcctg ccagaacgag aacaggccgt tgaccaggat gacgcagagg atggccacgc
     1381 cgagacggcc catgccctcg ccgggctgga gccagtcggc ggtgaacgcc agcgccgccg
     1441 ccagccagag gatgatcgcg aagaagtgcg tgaattcgcg cgccaggcgc agccacagcc
     1501 gctcgcgggc cacgtcctcg aggcggttcg ggccgaattc ggcgagccgg cgcgcggcct
     1561 cggacgccga caggccgtcg acgccgcttt tcaggctggc gagagcagcg tcgacaggaa
     1621 gttcatggat gcgcatggcg catcagcata cccggcgact cgatccttcg tgggtccgaa
     1681 ttcattcgga catcctgttg tccggctgaa gccggaccca cgggattcag acgccgaaca
     1741 gcccgtcctc gtagcccttc tcgcgcagc
//
LOCUS       DAORUQ010000363         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_113456_length_1752_cov_5.422510, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000363 DAORUQ010000000
VERSION     DAORUQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113456_length_1752_cov_5.422510"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..802)
                     /locus_tag="PLW13_16590"
     CDS             complement(2..802)
                     /locus_tag="PLW13_16590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY72051.1"
                     /translation="MQSNLNDRVYIVTGGSRGFGLAIAAALVQHGARVGLVSRNASAL
                     EAAVARLGADRAFAVGMDVSERADIARAFTRIKEHFGRLDGLINNAGLARPGAVENLD
                     AADVQAQVGTNLLGTVYCCQAAIPLLRGGDNPRIVNISSASAYHMDEMAHLSIYAATK
                     AAVERFSRDLRRELQGDGIGVTILRPGAAATEFAAGWDPSRFEPALKAWIDQGRLMDI
                     GMDAAHVGATVAYCLCCPPGVSIDLLEVRPFHQMPKPDPATLMAQHGG"
     gene            complement(818..1672)
                     /locus_tag="PLW13_16595"
     CDS             complement(818..1672)
                     /locus_tag="PLW13_16595"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY72052.1"
                     /translation="MSRTNVGFIGLGNIGQPMAKNLVNDAFDAWMYDVSEKALAELVA
                     LGARASTPAEIARNCPLIGICVRDDKDVDSLFYGDDGMLAHAAKDTVIAIHSTVTQAG
                     LARWAQDAAARGVHVIDAPITRGNGGMEPRFVCFLVGGEPEIVARARPLLDPSAENII
                     HAGPLGAGMALKLCNNLITNVEYLAVSEAWALAERCGLKIEVLRELGKANNVINDRMY
                     AFLSGRNTVAAMGDTALMDQHFGTFGRLAEKDLDAALESARDHDIELPGTRYTRAVIN
                     AVYTKKPY"
BASE COUNT          254 a          589 c          615 g          294 t
ORIGIN      
        1 ctcagccgcc gtgctgcgcc atcagcgtgg cgggatcggg cttcggcatc tggtggaacg
       61 ggcgcacctc gagcagatcg atcgagacgc ccggcgggca gcacaggcag taggccaccg
      121 tggcgccgac gtgcgcggcg tccatgccga tgtccatcag gcggccctgg tcgatccagg
      181 ccttcagcgc gggctcgaag cgcgacgggt cccagccggc ggcgaattcc gtcgcggccg
      241 cgcccgggcg caggatcgtc acgccgattc cgtcgccctg caactcgcgg cgcaggtcgc
      301 gcgagaagcg ctccaccgcg gccttggtgg ccgcgtagat cgacaggtgc gccatctcgt
      361 ccatgtggta ggcgctggcc gaggagatgt tcacgatgcg cgggttgtcg ccgccgcgca
      421 gcagcgggat cgcggcctgg cagcagtaga ccgtgccgag caggttggtg ccgacctgcg
      481 cctgcacgtc cgccgcgtcg aggttctcga ccgcgccggg gcgcgcgagc ccggcgttgt
      541 tgatcagccc gtcgaggcga ccgaaatgct ccttgatgcg cgtgaaggcg cgcgcgatgt
      601 ccgcgcgttc gctgacgtcc atgccgacgg caaaggccct gtcggcgccg aggcgcgcga
      661 cggcggcctc gagcgcggat gcgttgcggc tcacgaggcc gacgcgcgcg ccgtgttgca
      721 ccagcgccgc ggcgatcgcg aggccgaagc cgcgcgagcc gccggtgacg atgtagacgc
      781 gatcgttcag gttcgactgc atgcggaggc tccgggctca gtacggcttt ttcgtgtaca
      841 cggcattgat cacggcgcgc gtgtagcggg tgccgggcag ctcgatgtcg tggtcgcgcg
      901 cgctctcgag cgccgcatcc aggtcctttt cggccagcct gccgaaggtg ccgaagtgct
      961 ggtccatcag cgccgtgtcg cccatcgccg cgacggtgtt gcggccgctg aggaaggcgt
     1021 acatgcggtc gttgatcacg ttgttggcct tgccgagttc gcgcagcacc tcgatcttca
     1081 gtccgcagcg ctccgccagc gcccaggcct cgctcaccgc gaggtactcg acgttggtga
     1141 tcaggttgtt gcagagcttc agcgccatgc ctgcgccgag cggcccggcg tggatgatgt
     1201 tctcggcgga cgggtcgagc agcgggcggg cgcgcgcgac gatctccggc tcgccgccga
     1261 ccaggaagca gacgaagcgc ggctccatgc cgccgttgcc gcgcgttatg ggcgcgtcga
     1321 tcacgtgcac gccgcgagcc gcggcatcct gcgcccagcg cgccagcccc gcttgcgtga
     1381 cagtgctgtg gatcgcgatc acggtgtcct tcgcggcgtg cgcgagcatg ccatcgtcgc
     1441 cgtagaacag cgaatccacg tccttgtcgt cgcgcacgca gatgccgatc agggggcagt
     1501 tgcgcgcgat ctccgcgggg gtggaggcgc gtgccccgag cgcgacgagc tcggcgaggg
     1561 ccttctcgga cacgtcgtac atccaggcgt cgaacgcatc gttgaccagg ttcttcgcca
     1621 tcggctggcc gatgttgccc agaccgatga aacccacgtt ggtcctgctc atatcgctct
     1681 ccttcgctga agaaaaaaca ttgtaggaca aatctaatat ttgtaacatc cgtggcacca
     1741 actcctgtcg cc
//
LOCUS       DAORUQ010000364         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_115055_length_1738_cov_4.279857, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000364 DAORUQ010000000
VERSION     DAORUQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115055_length_1738_cov_4.279857"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLW13_16600"
     CDS             complement(<1..197)
                     /locus_tag="PLW13_16600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY72053.1"
                     /translation="MTVPADRVLIIVPAWNEARNVGATVREIAGVDGSYDVIVVDDGS
                     TDATAEVARRAGAKVLSLPFNL"
     gene            complement(247..>1738)
                     /locus_tag="PLW13_16605"
     CDS             complement(247..>1738)
                     /locus_tag="PLW13_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72054.1"
                     /translation="TNPGGALGFYPAAWHGAVALIVQLSGVAIPVAVNAMTLVVAALI
                     WPLGVVLLTRTLFGAGRVLVVSAGILATAFPLFPMLLMDYGVLYPFQLALALTPVALA
                     ATAQALGLAPEGGGLQRWWWALVLVGTLPGIMLAHPGAFVSWLALSAPMAVAFAVQRW
                     RASRTVRGRSLVIISFIAYMFAGALMLMALRPPSDARGWPTAMSIPSAVWEVLSVSAW
                     YGIAATVAALAVFAGVVWAIVARTVPALVGLGMYLVAAFLFVTVAAMPFGALRDAFTG
                     GWYNNIPRLAAMLPMVMVPLAAFGVARSASAIARIPVFTRARARLAPGWRVAVALLVA
                     AGGILSVQGPAMAQASASVSHMFALTPDSVLVNTDEYALIQRLDEHVPEGVAVAGSPW
                     TGASLSYALADRPVLMPHTLMEITDELALINERLDEASWNDAVCAAIDELGVGFVLDF
                     GTQEVHGAIHEFPGFDNLAESDRVRLVDAEGAAKLYEIVACGAVSG"
BASE COUNT          342 a          618 c          548 g          230 t
ORIGIN      
        1 aggttgaaag gcaggctgag cacctttgcg cccgcgcggc gcgcgacctc ggcggtcgcg
       61 tcggtcgagc catcatcgac gacgatcaca tcgtaggatc cgtccacgcc agcaatctct
      121 cgaaccgtcg cgcccacatt tcgggcttca ttccacgcgg ggacgatgat cagcacgcga
      181 tctgcaggga cggtcatggt gtcttgatcc tagcaaccgc gggccgaacg gcctcacgca
      241 gcgcactcac cccgataccg cgccgcacgc gacgatctcg tacagtttgg ccgcgccttc
      301 ggcatcgacg agcctgaccc gatcagattc ggcgaggttg tcgaagcccg ggaactcgtg
      361 gatcgcgcca tgcacttcct gcgtgccgaa gtccagcacg aagccgacgc ccagctcgtc
      421 gatcgcagcg cagacggcgt cgttccaact cgcctcatcc aagcgttcgt tgatcaacgc
      481 gagctcatcg gtgatttcca tgagtgtgtg cggcatcagc acgggtcgat cggcgagcgc
      541 gtaactcagc gaagcccccg tccacgggct ccccgcaact gccacgcctt ccggcacgtg
      601 ctcatcgagt cgctggatca gcgcatactc atcggtgttg acgagcacgg agtccggcgt
      661 caaagcgaac atgtgcgaga ccgatgcgct cgcctgcgcc attgcgggcc cttggacgct
      721 gagaattcct cctgcggcca caagcagcgc caccgccaca cgccaaccgg gggcgaggcg
      781 ggcgcgcgcc cgcgtgaaga ccgggatgcg cgcaatcgct gatgccgagc gcgccacacc
      841 gaaggcggcc agcggcacca tcaccatcgg cagcatggcc gcgaggcgcg ggatgttgtt
      901 gtaccacccg cccgtgaagg catcgcggag cgcgccgaat ggcatcgccg caaccgtgac
      961 gaacaggaag gccgcgacca gatacattcc cagcccaacc agcgctggca ccgtgcgcgc
     1021 gacgattgcc cacacgactc ccgcgaacac cgccagggcg gccaccgtcg cggcaatgcc
     1081 gtaccaggcc gacacagaca ggacttccca tactgcggaa ggaatgctca tcgcggtcgg
     1141 ccagccacgc gcgtcggacg gcgggcgcaa tgccatgagc atcagggcgc ccgcgaacat
     1201 gtaggcgatg aagctgatga tgacgagcga tcggccccgt accgtgcggg atgcgcgcca
     1261 gcgctgcacc gcgaacgcca cggccatcgg cgccgacagt gccagccaac tcacgaatgc
     1321 acccgggtgc gcgagcatga tccctggcaa cgtgcccaca aggacgagcg cccaccacca
     1381 gcgctggagc ccgcccccct ccggggcgag cccgagtgcc tgcgccgtcg ccgcgagcgc
     1441 aaccggtgtc aaggccagcg cgagctggaa tgggtacagc accccatagt ccatgagcag
     1501 catcgggaag agtgggaatg ccgtggccag gatgcccgcc gacacaacga ggacgcgtcc
     1561 cgccccgaac agtgtgcgcg tcaagagcac gacgccgagc ggccagatca gcgcggcgac
     1621 cacgagcgtc atcgcgttca cggcgaccgg gatggcaaca ccgctcagct gcacaatcag
     1681 tgcgacagcc ccgtgccatg cagcgggata gaaccccagc gcaccgccgg gattcgtc
//
LOCUS       DAORUQ010000365         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_116805_length_1723_cov_4.157074, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000365 DAORUQ010000000
VERSION     DAORUQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116805_length_1723_cov_4.157074"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..>1723
                     /locus_tag="PLW13_16610"
     CDS             281..>1723
                     /locus_tag="PLW13_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY72055.1"
                     /translation="MNISADNPREARARVALLAPAWGFLRPYWPRVLGASLALVFTAS
                     VTLSLGQGLRMLVDQGFVSGSAEQLNRAVLVFMLIVLLLAIGTFTRFYLVSWIGERVT
                     ADLRRAVFDHVLSLHPGFFETNGAGEIQSRITADTTLLQTVIGSSVSIALRNLLTLAG
                     GVVLLFVSNPRLTGIVLLSVPLVLLPIVVFGRRVRTLSRDSQDRLANAGAYLSEALGN
                     IKTLQAFNHEGADRERFAMHVEDTFRASIRRVVQRSWLTAMVIVLVLGAVGAMIWVGG
                     NDVIAGRTTPGELTAFIFYAVMVAAAAGALSEVIGDLQRAAGATERLMELLREAPLIR
                     APADPLALPARLRGEIELKELRFAYPSNPDRPAIDGITLHVPAGSSVALVGASGAGKS
                     TLVDLLLRFYEPDGGSILLDGIDTRRVDPQALRRHFALVPQQPVLFTGSVLENIRYGV
                     PGASEEAVMAAARAAFAEEFIARLPEGYASF"
BASE COUNT          225 a          588 c          618 g          292 t
ORIGIN      
        1 gcgcgagcct gcgccggcgc atcgccgagt tgcgcctcga gcgcgccggg gatgcccgcg
       61 cgctggcgat actcgaggcg ctggatgcct acagcaacga attgtgttcc tccgtgtagg
      121 agcgggccat gcccgcgaca ggagcgcgca cgaacgcatt cgaacaggtc gcgggcatgg
      181 cccgctctgg tccgtgtagg agcgggccat gcccgcgaaa ggagcacgca agattcattc
      241 gaacggttcg cgggcgtggc ccgctcctac gaggaaaata atgaacatat ctgccgacaa
      301 cccccgcgaa gcccgcgcgc gcgtcgccct gctggcgccc gcatgggggt tcctgcgccc
      361 gtactggccc cgcgtgctcg gcgcttcgct cgcgctggtg ttcaccgcga gcgtgacgct
      421 cagcctcggg caggggctgc gcatgctggt ggaccagggc ttcgtgtcgg gctccgccga
      481 gcagctgaac cgcgcggtgc tggtgttcat gctgatcgtg ctgctgcttg cgatcgggac
      541 cttcacgcgc ttctacctgg tgtcgtggat cggcgagcgc gtgacggcgg acctgcgccg
      601 cgcggtgttc gaccacgtgc tgtcgctgca tccgggcttc ttcgagacca acggcgcggg
      661 cgagatccag tcgcgcatca ccgccgacac cacgctgctg cagacggtga tcggctcctc
      721 ggtctcgatc gcgctgcgca acctgctgac gctcgccggc ggcgtggtgc tgctgttcgt
      781 ctcgaacccg cgcctgaccg gcatcgtgct gctcagcgtg ccgctggtgc tgctgccgat
      841 cgtggtgttc ggccggcgcg tgcgcacgct gtcgcgcgac agccaggacc ggctcgcgaa
      901 tgccggcgcc tatctcagcg aggcgctcgg caacatcaag accttgcagg ccttcaacca
      961 cgagggcgcg gaccgcgagc gcttcgcgat gcacgtcgag gacaccttcc gcgcctcgat
     1021 ccgccgcgtg gtgcagcgtt cctggctcac tgcgatggtg atcgtgctgg tgctgggcgc
     1081 cgtgggcgcg atgatctggg tcggtggcaa cgacgtgatc gccgggcgca cgaccccggg
     1141 agagctcacg gccttcattt tctatgcggt gatggtggcg gccgcggccg gggcgctgtc
     1201 ggaagtgatc ggtgacctgc agcgcgccgc cggcgccacc gagcgcctga tggaactgct
     1261 gcgcgaggcg ccgctgatcc gcgcgccggc cgatcccctg gcgctgccgg cgcgcctgcg
     1321 cggcgagatc gagctgaagg agctgcgctt cgcctaccct tcgaacccgg atcgccccgc
     1381 gatcgacggc atcacgctgc acgtgccggc cggatccagc gtggcgctgg tgggcgcctc
     1441 gggtgcgggc aagtcgacgc tggtcgatct gctgctgcgc ttctacgagc ccgacggcgg
     1501 cagcatcctg ctcgatggca tcgatacgcg tcgcgtcgat ccgcaggcgt tgcgccggca
     1561 tttcgcgctg gtgccgcagc agccggtgct gttcacggga tcggtgctcg aaaacatccg
     1621 ctacggcgtc cccggcgcga gcgaggaggc ggtgatggcg gcggcgcgcg cggccttcgc
     1681 cgaggagttc atcgcgcggc tgcccgaggg ttacgccagc ttc
//
LOCUS       DAORUQ010000366         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_117151_length_1720_cov_5.523123, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000366 DAORUQ010000000
VERSION     DAORUQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117151_length_1720_cov_5.523123"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLW13_16615"
     CDS             complement(<1..219)
                     /locus_tag="PLW13_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72056.1"
                     /translation="MSTPRTLEEHLQRVCELPTDTRRIAFFDLDRTLVAGYTMAALVF
                     EQARRGLLSPRQLARQSLNYIQYGRARIG"
     gene            complement(335..514)
                     /locus_tag="PLW13_16620"
     CDS             complement(335..514)
                     /locus_tag="PLW13_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72057.1"
                     /translation="MSTLPLAQRISRNLLGFARQKPAFVARDDMILSRSRVFNWAAAG
                     MPPWCVLPASACAPR"
     gene            complement(511..1446)
                     /locus_tag="PLW13_16625"
     CDS             complement(511..1446)
                     /locus_tag="PLW13_16625"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY72058.1"
                     /translation="MKRRESAFAPHVRIGQRHGHEARFDNHEGGQMEQDLLRLDGRIV
                     VVSGAGGGGLGTTVTRMAARAGATVIGVSRNQANLDQHLGPLVSQGLAVIPVAADVET
                     AAGVATVMDSVRNTRGDLHGLVSVVGGGTPPTWGPATDVSRENWRALFARNLDSMFFI
                     SQAVAARLREQGRPGSLVAISSITGLGAQPYNVAYGAAKAAVLSVVRTMALELAASGI
                     RVNAVAPGAMASPASLLPPDPVLERRAIPMGRKGNVEEIAATVLFLLSDMATYMTGQC
                     LTVDGGISLKWSHLAEDNTPVLVTNQAFLDAMKGV"
     gene            1540..>1720
                     /locus_tag="PLW13_16630"
     CDS             1540..>1720
                     /locus_tag="PLW13_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY72059.1"
                     /translation="MTQRGDGETEEGFSMSNAVNDGRRIVAGRSIRWDEERANEAYAQ
                     GFWVRETLAEALARAA"
BASE COUNT          294 a          577 c          593 g          256 t
ORIGIN      
        1 gccgatgcgc gcgcggccgt actggatgta gttgagactc tggcgggcga gctggcgcgg
       61 cgagagcagc ccgcggcgcg cctgttcgaa caccagcgcg gccatcgtat agccggcgac
      121 cagggtacgg tcgagatcga agaaggcgat tcgacgggtg tccgtgggca actcgcacac
      181 gcgctgcagg tgctcctcga gggtccgggg cgtcgacatg gcaattcctc cgcgcgttgt
      241 ctgcaagatg gtgctgcaag cgatgtgcca cggaaaaacg cgctgaatcc cgacgtttcg
      301 ggccgtctcg cgcaccgcga tggcgcgccg cgcttcagcg cggtgcgcag gcactggccg
      361 gcagcacgca ccagggcggc atgccggcgg ccgcccaatt gaacacacgc gagcgtgaca
      421 ggatcatgtc atcacgagcg acgaacgcag gcttctgccg cgcgaagccg aggaggttcc
      481 ggctgatgcg ctgcgccagt ggcagcgtgc tcacacgccc ttcattgcat cgaggaacgc
      541 ctggttggtg acgagcacgg gcgtgttgtc ttccgccagg tgcgaccact tgagactgat
      601 tccgccgtcc acggtcaggc actggcccgt catgtacgtt gccatgtctg acaacaggaa
      661 gagcacggtt gcggcgatct cctcgacgtt tcccttgcgc cccatcggga tcgcgcggcg
      721 ctccagcacc ggatcgggcg gcagcagcga cgcgggggaa gccatcgcgc cgggcgccac
      781 ggcattgacg cggatgccgg acgccgccag ttcgagcgcc atggtccgca ccacggacag
      841 caccgctgcc ttcgccgcgc cataggcaac gttgtagggt tgcgcgccga ggccggtgat
      901 cgacgagatc gccaccagcg agcccggtcg cccctgctcg cgcaaccgcg ccgccacggc
      961 ctggctgatg aagaacatgg agtcgaggtt cctggcgaac agcgcgcgcc aattctcccg
     1021 ggaaacatcg gttgcgggcc cccacgtcgg cggtgtcccg ccgcccacga cgctgacgag
     1081 cccatggaga tctccacgcg tgttgcgcac gctatccatg acggtggcca cgccggcggc
     1141 ggtctccacg tcggccgcga ccggaatgac cgcaagcccc tgcgacacca gcggaccgag
     1201 atgctggtcg agattggcct ggttgcgact caccccgatc acggtcgcac cggcccgcgc
     1261 ggccatgcgc gtgacggtgg tccccagccc gccgccgccc gcgcccgaca caacgacgat
     1321 gcggccatcg agccgcaaga gatcctgttc catctgcccc ccttcgtgat tgtcgaaccg
     1381 ggcctcgtgg ccatgcctct gcccgatgcg cacgtgcggc gcgaaggccg attccctgcg
     1441 tttcacgcta gcaggaacgc catgcccgga caacagcagc gggagcaacg cgcaacgagg
     1501 tacggtacaa tttttcggat tggccgccga cgaatgccga tgacgcagcg tggcgatggc
     1561 gagaccgagg aaggattcag catgtcgaac gcggtgaatg acgggcgtcg gatcgtggcg
     1621 ggcaggtcga tccgctggga cgaggagcgc gcgaacgagg cctatgcgca ggggttctgg
     1681 gttcgcgaga cgctggccga ggcgctggcg cgcgcagcat
//
LOCUS       DAORUQ010000367         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_120954_length_1689_cov_4.739902, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000367 DAORUQ010000000
VERSION     DAORUQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120954_length_1689_cov_4.739902"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PLW13_16635"
     CDS             complement(<1..1505)
                     /locus_tag="PLW13_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY72060.1"
                     /translation="MVATQGLPEGLSGVRVLDTGRDAFLERAALIETAERAIDLQYYI
                     WNSDVTGIYLAHRLYAAAGRGVHVRLLLDDVNVAGRDAALALLDSHPNIEVRVYNPFA
                     RRHGAGRVLNLLGDFKRLNRRMHVKTFTVDDSVTIIGGRNIGDEYFDAHAHLNFRDRD
                     VVAIGPVVDTTAQMFDDFWNAALSRPIAALTDTQPDAVGKPDLTVFERAAHTIEALHG
                     ALPQRSDAPQRLRTSFAGMTQAQATLVHDRPAAPETVAARVRAKMQSLAPIIPAGMEL
                     QPIYDQPREVAKSVGGFLVSVAQAVGIVLAVLLLFMGLRTGVIIGAVLLITVAGTLFV
                     MQLCGIELQRISLGALVIALGMLVDNAIVVAEGMLVRMQAGMSAPQAARESVAKTAWP
                     LLGGTAIGILAFSAIGLSPDATGEFAGSLFWVILISLLLSWLTAISTTPLLCALLLEP
                     AAASATPRDPYAGAGFRLFRRLVTVALHHRLVTLGAAAALFLAALAGFGFV"
BASE COUNT          276 a          575 c          611 g          227 t
ORIGIN      
        1 ttgacgaaac cgaaaccggc cagtgcggcg aggaacagcg ccgccgcggc accgagcgtt
       61 acgaggcgat ggtgcagcgc caccgtcacg aggcgccgga acaggcgaaa gcccgcgccg
      121 gcgtaggggt cgcgcggcgt cgcgctcgcg gcggcgggct cgagcagcag cgcgcacagc
      181 agcggcgtgg tgctgatcgc cgtgagccac gagagcagca gcgagatcag gatcacccag
      241 aacagcgatc cggcgaattc cccggtggcg tcgggcgaga ggccgatggc ggagaaggcg
      301 agaatgccga tggcggtgcc gccgagcagc ggccacgcgg tcttggcgac gctctcgcgc
      361 gcggcctgcg gcgccgacat gcccgcctgc atgcgcacca gcatgccttc ggcgaccacg
      421 atcgcgttgt cgaccagcat gccgagcgcg atcaccagcg cgccgagcga gatgcgctgc
      481 agctcgatgc cacagagctg catcacgaac agcgtgccgg ccacggtgat cagcagcacc
      541 gcgccgatga tcacgccggt gcgcaggccc atgaacagca gcagtaccgc gagcacgatg
      601 cctaccgcct gcgccacgct gaccaggaag ccgcccaccg acttggccac ctcgcgcggc
      661 tggtcgtaga tgggctgcag ctccatgccc gccgggatga tcggggcgag cgattgcatc
      721 ttcgcccgca cccgcgcggc gacggtctcg ggtgcggcgg gtcggtcgtg caccagcgtc
      781 gcctgcgcct gggtcatccc cgcgaaactc gtgcgcaggc gctgcggcgc atcgctgcgc
      841 tgtggcagcg cgccgtgcag tgcctcgatg gtgtgcgcgg cacgctcgaa caccgtgagg
      901 tccggtttgc ccacggcatc gggctgcgtg tcggtcagcg ccgcgatcgg ccggctcagc
      961 gcggcattcc agaagtcgtc gaacatctgc gcagtcgtat cgaccacggg gccgatggcc
     1021 accacgtcgc ggtcgcggaa attcaggtgt gcatgggcat cgaagtactc gtcgccgata
     1081 ttgcgcccgc cgatgatcgt gacggaatcg tcgaccgtga aggttttcac gtgcatgcgc
     1141 cggttcaggc gcttgaagtc tcccagcagg ttcaggacgc gcccggcgcc gtggcgccgc
     1201 gcgaacgggt tgtagacgcg gacctcgatg ttcgggtgcg agtccagcag cgcgagtgcg
     1261 gcgtcgcgcc cggccacgtt cacatcgtcg agcagcaggc gcacgtgcac gccgcgcccg
     1321 gcggccgcgt acagccgatg ggccaggtag atgcccgtca catcgctgtt ccagatgtag
     1381 tactgcaggt cgatggcgcg ttcggcggtc tcgatcagcg cggcgcgctc gaggaaggca
     1441 tcgcggccgg tgtccagcac gcgcacgccg ctcaaaccct ccggcaggcc ttgcgtggcg
     1501 accatctgcc cgagcgtggt agcggcgccc gcggtgaaat gcgtttgcag cgccagcggc
     1561 gccggctggg ccggcagccg ggcacagcca ggcagcagaa cgaacaacgc aacgacgcac
     1621 cagcgtagcg cctgcacgtg caggcgcacg gcgtggccgg tgcgagcgca ctgcgcgggg
     1681 attgggccg
//
LOCUS       DAORUQ010000368         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_129683_length_1622_cov_5.487556, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000368 DAORUQ010000000
VERSION     DAORUQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129683_length_1622_cov_5.487556"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1622
                     /locus_tag="PLW13_16640"
     CDS             20..>1622
                     /locus_tag="PLW13_16640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HQY72061.1"
                     /translation="MTRSELSAGLPETAARVVCLDRDWPLIEGHGIGAFASGADAGSL
                     AYVIYTSGSTGTPKGVQVTHRAISRLVRNTDFIDIRPTDRLAQASVASFDAATFEIWG
                     ALLNGAQVIGVSREVLLSPREFSEFLRTRGITVLFLTTALFNQMAREAPGAFRSLRCV
                     LFGGEAVDPDWVRAVLRDGPPQRLLHVYGPTEVTTFSTWHLVAGLDQAATTVPIGRPI
                     ANTTAYVLDEHREPVPVGVPGELYLGGDGLARGYLKRPELTAERFVDHPFSEEKGARL
                     YRTGDWVRYLPDGAIEYIGRRDNQVKIRGYRIEPGEIEAALVRHPAVREAVAMAREDE
                     PGDKRLVAYVTPGEAIPSPPELRAWLRERLPEYMVPGAFVVLDRFPLSPNGKVDRGAL
                     PAPSGERQLESEFVAPRGRLAAAIAAVWSDVLGVARVGAHDNFFDLGGNSLLLVSVHE
                     RLQQALDMRFRVVELFQYPTIESLARHFAGEIPAPATLAAARERAGRRRDSAGGFGAI
                     AVIGMAGRFPGADSVEAFWRNLCDGV"
BASE COUNT          222 a          543 c          592 g          265 t
ORIGIN      
        1 tgcgggggcg caggtggtgg tgacccgcag cgagttgtcg gcgggtctgc ctgagactgc
       61 cgcgcgggtc gtgtgcctgg accgggactg gccgttgatc gaaggccatg gcatcggggc
      121 gttcgcgagc ggtgcggacg ccgggagcct tgcctacgtg atctacacct cgggctcgac
      181 cggcacgccc aagggggtgc aagtgacgca ccgggcgatc agccgcctgg tgcgcaacac
      241 cgacttcatc gacatccggc ccaccgatcg ccttgcgcag gcttcggtgg cgtccttcga
      301 tgcggcgaca ttcgagatct ggggcgccct gctcaacggc gcgcaggtga tcggcgtgtc
      361 gcgcgaggtc ctgctcagtc cgcgcgagtt cagcgagttc cttcgcacgc gcggcatcac
      421 ggtgctgttc ctgacgacgg cgctgttcaa ccagatggcg cgcgaggcgc ccggagcttt
      481 ccgttcgctg cgctgcgtgc tcttcggcgg ggaggcggtg gatcccgact gggtccgggc
      541 ggtattgcgt gacggccctc cgcaacggtt gctgcacgtc tatgggccga cggaggtgac
      601 caccttctcg acctggcacc tggttgccgg gctcgatcag gcggcgacca cggttccgat
      661 cggacgaccg atcgccaaca ccacggccta cgtgctcgac gagcatcgcg agccggtgcc
      721 cgtgggcgtg cccggcgagc tttatctggg aggcgatgga cttgcgcggg gctacctcaa
      781 gcgcccggag ctgaccgccg agcgctttgt cgaccacccg ttcagcgaag agaagggcgc
      841 gcgcctctac cgtaccggcg actgggtgcg ctacctgccc gacggggcga tcgagtacat
      901 cgggcggcgc gacaaccagg tgaagatccg cggctatcgc atcgagccgg gcgagatcga
      961 ggcggcgctg gtccggcacc cggcggtgcg cgaggcggtg gcgatggccc gcgaggacga
     1021 gccgggcgac aagcggctgg tggcttacgt cactccgggt gaagccattc cgagtccgcc
     1081 ggaactgcgc gcctggctgc gtgagaggct gccggagtac atggttccgg gcgccttcgt
     1141 cgtgctcgac cggtttccgc tctcacccaa cggcaaggtc gaccgcggcg cgctgccggc
     1201 cccgagtggc gagcgccagc tcgaaagcga gttcgtcgcg ccgcgtggca ggcttgcggc
     1261 ggccatcgcc gcagtctgga gcgacgtgct cggagttgca cgggtcggcg cacacgacaa
     1321 cttcttcgac ctgggcggca actcgctgct gctcgtcagc gtccacgagc gcctgcagca
     1381 ggcgctcgac atgcgcttcc gggtcgtcga actgtttcag tatccgacta tcgagtccct
     1441 ggcgcgtcac ttcgccggcg agatccccgc gcccgcgacc ctggccgccg cacgtgagcg
     1501 cgcaggccgg cgccgcgact cggcgggcgg cttcggcgcg attgcggtaa tcggcatggc
     1561 agggcgcttt cccggcgccg acagcgtcga ggcgttctgg cgcaacctct gcgacggcgt
     1621 cg
//
LOCUS       DAORUQ010000369         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_131365_length_1610_cov_6.644373, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000369 DAORUQ010000000
VERSION     DAORUQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131365_length_1610_cov_6.644373"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..959)
                     /locus_tag="PLW13_16645"
     CDS             complement(9..959)
                     /locus_tag="PLW13_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQY72062.1"
                     /translation="MTTTSEAVTRAARIFVAGHRGMVGSAIVRRLQRGGYHNLLLRDR
                     AGLDLTDQAAVEGFFATERPDYVFIAAAKVGGIQANNTLRAEFLYQNLMIESNVIHAA
                     WRAGVERLLFLGSSCIYPRDCPQPIREEYLLTGPLEPTNEPYAIAKIAGIKLCESYNR
                     QYGTRYVSAMPTNLYGPNDNYDLASSHVLPALIRKAHEAKARGDSELVVWGSGRPLRE
                     FLYVDDMADACVFLMERGISEGLFNVGTGSDVSIRELAQTVMDVVGFQGRIVFDAAKP
                     DGTPRKLLDVGRLRELGWCASTGLREGIALAYRDFLNARR"
     gene            complement(943..>1610)
                     /locus_tag="PLW13_16650"
     CDS             complement(943..>1610)
                     /locus_tag="PLW13_16650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQY72063.1"
                     /translation="QEVPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGIL
                     FNHESPVRGETFVTRKITRALARIKLGLQDCLYLGNLDALRDWGHARDYVEMQWLMLQ
                     QETAEDYVIATGEQHSVREFVDLAAAELGLRITWSGSGAAETGADQHGRVIVRVDPRY
                     FRPTEVETLLGDSTKARVQLGWTPRTSFGELVAEMIREDLKSAERDELVRKHGFPAYD
                     YHE"
BASE COUNT          247 a          543 c          546 g          274 t
ORIGIN      
        1 cagggccgtc atcggcgggc gttcaggaaa tcccggtacg ccagcgcaat gccctcgcgc
       61 aggccggtcg aggcgcacca gcccagctcg cgcaggcggc cgacgtcgag cagcttgcgc
      121 ggcgtgccgt cgggcttggc ggcatcgaac acgatgcggc cctggaaacc caccacgtcc
      181 atcaccgtct gagcgagctc gcggatgctg acatcgctgc ccgtgcccac gttgaagagg
      241 ccctcggaaa tgcccctctc catcaggaac acgcaggcat cggccatgtc gtcgacgtag
      301 aggaattcgc gcagcggccg gcccgagccc cataccacca gctcactgtc gccgcgcgct
      361 ttggcctcgt gggccttgcg gatcagtgcc ggcagcacgt ggctgctcgc gaggtcgtag
      421 ttgtcgttcg gtccgtagag attggtcggc atcgcggaaa cgtaacgcgt gccgtactgg
      481 cggttgtagc tctcgcagag cttgatgccg gcgatcttcg cgatggcgta gggttcgttg
      541 gtgggttcca gcggaccggt gagcaggtat tcctcgcgga tcggttgcgg gcagtcgcgc
      601 gggtagatgc agctcgagcc caggaacagc agccgctcga cgccggcgcg ccacgccgcg
      661 tggatcacgt tgctctcgat catcaggttc tggtacagga actccgcgcg cagcgtgttg
      721 ttggcctgga tgccgcccac cttcgccgcg gcgatgaaga cgtagtccgg ccgttcggtg
      781 gcgaaaaaac cttccaccgc ggcctggtcc gtgagatcga gcccggcacg gtcgcgcagc
      841 agcaggttgt ggtagccccc gcgctgcagc cggcgcacga tcgccgagcc caccatgccg
      901 cggtggcccg cgacaaagat gcgcgccgcg cgcgtcacgg cctcactcgt ggtagtcata
      961 ggcgggaaaa ccgtgcttgc ggaccagctc gtcgcgctcg gcgctcttga ggtcctcgcg
     1021 gatcatctcg gccaccagct cgccgaagct ggtgcgcggc gtccagccga gctgcacgcg
     1081 cgccttggtc gaatcgccga gcagcgtctc gacctcggtg ggccggaaat agcgcgggtc
     1141 cacccgcacg atcacccgcc cgtgctggtc ggcgccggtc tccgcggcgc cgctgcccga
     1201 ccaggtgatg cgcagcccca gctcggcggc ggcaaggtcc acgaactcgc gcacactgtg
     1261 ctgttcgccg gtggcaatga cgtagtcctc ggccgtttcc tgctgcagca tcagccactg
     1321 catctcgacg tagtcgcgcg catggcccca gtcgcgcagc gcgtcgaggt tgccgaggta
     1381 gaggcagtcc tgcagcccca gcttgatgcg cgccagcgcg cgcgtgatct tgcgcgtcac
     1441 gaaggtctcg ccgcgcaccg gcgattcgtg gttgaacagg atgccgttgc aagcgtacat
     1501 cccgtaggcc tcgcggtaat tcacggtgat ccagtaggcg tagagcttgg cgacggcata
     1561 ggggctgcgc gggtagaacg gcgtggtctc cttctgcggc acttcctgca
//
LOCUS       DAORUQ010000370         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_131532_length_1609_cov_3.761905, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000370 DAORUQ010000000
VERSION     DAORUQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131532_length_1609_cov_3.761905"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1609
                     /locus_tag="PLW13_16655"
     CDS             <1..>1609
                     /locus_tag="PLW13_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY72064.1"
                     /translation="EDKVLLRGAFTAIERDGYIHNILDDEDLGETDRQSGRISLAVKP
                     NEQLENITMVQFSNTDSTNTGASYTYSVYQCGDTNNGVALTCNAGLLFSPALDAAFQT
                     PGAWASYLAAHPDAYPAGLPAYVDEQRRIGAYRTRHPAGADHESDDWMVTNTTTYELT
                     GDMRLKNIFGASRSQVDSEQPQLGAPFVTILTKNLVTGESGNELDVESYSNELQLQGA
                     ALDEALNYIAGLYLQRAQTDTLWPQTYFDVSPVLAPSSVTSNFRATNETQAVYAQGSY
                     DLGGLTGVENLRFTAGARYTWEHVDFEQLGASTYTADAPDQSETYSDPSWELGLEYQA
                     TDSLLTYIKTRGSFRSGGFNGAAPPIDADATGGGNIFDSETTQDVEAGLKFRGTVADR
                     AAMLNVAIYNQWIDDVQRVEFPDPDGAGPVASISVTANVPEEEIKGIEVEASIMALSW
                     LELGVAGAYTDAEFTDNEVNLFGNVYSYGPVGDTPEQSGVAWARIEFPTGDGVGRISL
                     RGEIYAQSEQFFSNTYDSLGPGTRLDGY"
BASE COUNT          312 a          539 c          513 g          245 t
ORIGIN      
        1 cgaggacaag gtgctgctgc gcggggcctt caccgccatc gagcgcgacg gctacatcca
       61 caacatcctg gatgacgagg acctgggcga gaccgatcgc cagagcgggc gtatctcgct
      121 ggcggtgaag ccgaacgagc agctcgagaa catcacgatg gtgcagttct ccaacaccga
      181 cagcaccaac accggcgcct cgtacaccta cagcgtctac cagtgcggcg acaccaacaa
      241 tggcgtggcg ctcacctgca atgccgggtt gctcttcagc ccggcgctcg acgcggcttt
      301 ccagactccc ggcgcgtggg cctcgtatct ggccgcgcat cccgacgctt atccggcagg
      361 tctgcccgcc tatgtggacg agcagcggcg catcggcgcc tacaggaccc ggcatcccgc
      421 cggggcggac cacgagagcg atgactggat ggtgaccaac accaccacct acgagctgac
      481 cggggacatg cgcctgaaga acatcttcgg cgcctcgcgc tcgcaggtgg attccgagca
      541 gccacagctc ggcgcgccct tcgtcaccat cctcacgaag aacctcgtga ccggcgaatc
      601 cggcaacgag ctcgacgtgg agtcgtattc gaacgaactg cagctccagg gcgcggcgct
      661 ggacgaggca ctgaactata ttgccggcct ctacctgcag cgcgcgcaga ccgacacgct
      721 gtggccgcag acctacttcg acgtgagccc cgtgctggcg ccgagcagcg tcaccagcaa
      781 tttccgcgcg accaacgaaa cgcaggccgt ctacgcacag ggatcctacg acctgggcgg
      841 cttgaccggg gtggagaacc tgcgcttcac cgccggtgcg cgctacacct gggagcacgt
      901 tgacttcgaa caactcggcg cttccaccta cacggcggat gcgccggacc agtccgagac
      961 ctacagcgat ccgagctggg agctcggcct ggaataccag gccaccgaca gcctgctgac
     1021 ctacatcaag acgcgcggca gcttccgcag cgggggcttc aacggtgccg cgccgccgat
     1081 cgatgccgat gcgaccggtg gtggcaacat cttcgattcc gagacgacgc aggatgtcga
     1141 ggccgggctg aaattccgcg gcacggtggc cgatcgcgca gccatgctga acgtggcgat
     1201 ctacaaccag tggatcgacg atgtgcagcg cgtcgagttc ccggacccgg atggtgccgg
     1261 tcccgtcgcg tccatctcgg tgaccgccaa cgtgcccgag gaagagatca agggcatcga
     1321 ggtggaggca tccatcatgg cgctgtcctg gctggagctg ggggtggcag gcgcctatac
     1381 cgatgccgag ttcacggata acgaggtcaa cctgttcggc aacgtataca gctacggccc
     1441 ggtgggtgac acgccggaac aatccggggt cgcctgggcg cggatcgaat tcccgaccgg
     1501 cgacggtgtc gggcggatca gcctgagggg cgagatctac gcgcagagcg aacagttctt
     1561 ctccaacacc tacgactcgc tcgggccggg cacccgcctc gatggttac
//
LOCUS       DAORUQ010000371         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_135949_length_1578_cov_6.804334, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000371 DAORUQ010000000
VERSION     DAORUQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135949_length_1578_cov_6.804334"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..391)
                     /locus_tag="PLW13_16660"
     CDS             complement(8..391)
                     /locus_tag="PLW13_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY72065.1"
                     /translation="MITSLDQFCINVSDLERAVRFYEQALGLAVTHRIEIPGVSEVVL
                     AGASGNRIQLARHHDHEGAIDHGNALWKLYLNTDDCAGLYARAIEAGAEAVSPPQLLA
                     QWQVTAAFIKDPDGYQIEIIQQHAE"
     gene            complement(505..>1578)
                     /locus_tag="PLW13_16665"
     CDS             complement(505..>1578)
                     /locus_tag="PLW13_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQY72066.1"
                     /translation="PQGAHAARRAPGGRRFVTVATTLRINMTGAPASPETQGERYRAA
                     IEMAAFADRHGFSIVNVEEHHCAAIGWLPSPLVMAGLIAGRTARVRIRVSALLITLYD
                     PIRLAEDIAILDLASNGRVSIVAGQGYRPIEYHALDRDWASRGMTSDFILQTLLQAWQ
                     GEPFEYRGRTVTISPVPQTRPMPPFAYGGMSAAAARRAARFGLDFHPPAHMPELVAIY
                     EDELKRLGKSGHAEWPREGTSLVFLDEDPEAAWQEIGPYLLAEAAEYGGWAREGVQRP
                     FASADLTLDKLRAEGRYEILTPAQCLRRIGAAQGEYTVILHPMAGGIPLPRAWNCLRL
                     YADKVLQPLGIGVRDSAGLPQHA"
BASE COUNT          222 a          555 c          551 g          250 t
ORIGIN      
        1 gccgcgttca ctccgcatgc tgctggatga tctcgatctg gtagccgtcg ggatccttga
       61 tgaacgccgc cgtcacctgc cactgcgcga ggagttgcgg cggcgagacg gcctcggcgc
      121 cggcctcgat cgcccgcgcg tagaggcccg cgcaatcgtc ggtgttcaga tacagcttcc
      181 acagcgcgtt gccgtgatcg atggcgccct cgtggtcgtg gtggcgggcg agctggatgc
      241 ggttgccgct ggcgcccgcg agcacgacct cgctcacgcc cgggatctcg atgcggtgcg
      301 tgaccgcgag ccccagcgcc tgctcgtaga agcgcacggc acgctcgaga tcgctgacgt
      361 tgatgcagaa ctggtcgagg ctggtaatca tcgttttctc cgcgatgtca ttggcgcgac
      421 ccacggtaac cgcgggccgg gtgcaggaca atttacggat tgctcacgtt cgcccccggg
      481 ccgacgatga cgcccgcgcc gccgtcaggc gtgttgcggc aggccagcgg agtcccgcac
      541 accgataccc agcggctgca acaccttgtc ggcatagagc cgcaggcagt tccacgccct
      601 cggcaacgga atgccgcccg ccatcgggtg caggatcacc gtgtactcgc cttgcgccgc
      661 gccaatgcgg cgcaggcatt gcgcgggcgt gaggatctcg tagcgccctt cggcgcgcag
      721 cttgtcgagc gtgaggtccg ccgaggcgaa gggccgctgc acgccctcgc gggcccagcc
      781 gccgtactcg gcggcctcgg cgaggagata cggaccgatc tcctgccagg cggcctcggg
      841 atcctcgtcg agaaacacca ggctggtgcc ttcgcgcggc cactcggcat ggccgctctt
      901 gccgaggcgc ttcagctcgt cctcgtagat cgcgaccagc tcgggcatgt gcgccggcgg
      961 gtggaagtcc aggccgaagc gcgcggcgcg ccgcgcggcc gccgcactca tgccgccgta
     1021 ggcgaagggc ggcatcgggc gcgtctgcgg caccggactg atcgtcaccg tgcgcccccg
     1081 gtactcgaag ggctccccct gccacgcctg cagcagcgtc tgcaggatga agtccgaagt
     1141 catgccccgg ctcgcccagt cgcgatccag tgcgtggtac tcgatcggcc ggtagccctg
     1201 cccggccacg atggacacgc gcccgttgct cgcgagatcg aggatcgcga tgtcctcggc
     1261 gaggcgtatc gggtcgtaca gcgtgatcag cagcgcgctc acgcggatgc gcacgcgcgc
     1321 ggtgcgcccc gcgatcagcc ccgccattac cagcggcgag ggcagccagc cgatcgcggc
     1381 gcagtgatgc tcctcgacgt tcacgatgct gaagccgtgg cggtcggcaa aggccgccat
     1441 ctcgatggcg gcgcggtagc gctcgccctg cgtctcggga ctggctggcg ccccggtcat
     1501 gttgatgcgc agcgtggtgg cgaccgtcac gaaacgtctc ccgccgggcg cgcgccgcgc
     1561 ggcgtgcgct ccttgtgg
//
LOCUS       DAORUQ010000372         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_135956_length_1578_cov_5.442548, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000372 DAORUQ010000000
VERSION     DAORUQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135956_length_1578_cov_5.442548"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /locus_tag="PLW13_16670"
     CDS             complement(<1..>1578)
                     /locus_tag="PLW13_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HQY72067.1"
                     /translation="LGCLVLAKLGSIWAPFLLKHAVDTMGAGGAAWLKAALGLVAAYG
                     AARFANVMFGEIRDTLFGRVTERAMRRVGLRVFEHLHGLDLDFHLERRTGGLARDIER
                     GTNGISFLMRFFVFNIVPTLFEILVVAGLLAWNYGIAYAAITLAAVLAYIAFSIVATE
                     WRTTYVRAANLADSASNTLAVDSLLNYETVKYFGNEAHEAARYDAELGRWEEARRKNR
                     LTLFALNAGQALIVALAMTASMALAARDVSRAQMSVGDFVLINAFMMQLFMPLNFLGM
                     VYREIKGSLAAIEEMFGLLAVRPRVADRPGARALAVSGARVEFRDVAFRYHAEREILR
                     GVNLCIEPGTKLAVVGASGAGKSTLVKLLFRFHDASEGAILIDGQDIRDVTQQSLRAA
                     IGIVPQDTVLFNDTLLENIRFGRPGASDADVQQAVRMAHLADFVAQLPQGLATRVGER
                     GLKLSGGERQRVAIARTILKRPPILVFDEATSSLDSKAERLILEALAEIAHGHTSLVI
                     AHRLSTVVDADCIAVLDG"
BASE COUNT          245 a          557 c          541 g          235 t
ORIGIN      
        1 gccgtccagc accgcgatgc agtcggcgtc gaccacggtc gagagccggt gcgctatcac
       61 caggctggtg tggccgtgcg cgatctcggc gagtgcctcc agaatgaggc gctcggcctt
      121 gctgtcgagc gatgaggtgg cctcgtcgaa caccagtatc ggcgggcgct tgaggatggt
      181 gcgcgcgatc gccacgcgct ggcgctcgcc gccggagagt ttcaggccgc gctcgcccac
      241 gcgcgtggcc agcccctgcg gcagctgcgc cacgaagtcg gccaggtggg ccatgcgcac
      301 ggcctgctgc acgtcggcgt cgctcgcccc cgggcgcccg aagcggatgt tctcgagcag
      361 cgtatcgttg aagagcacgg tgtcctgcgg cacgatgccg atcgccgcgc gcagcgattg
      421 ctgcgtgacg tcgcggatat cctggccgtc gatcaggatc gcgccctcgc tcgcgtcgtg
      481 gaagcggaac agcagcttca ccagcgtcga cttgccggcc ccgctcgcgc ccaccaccgc
      541 gagcttggtg cccggttcga tgcagaggtt gacgccgcgc aggatctcgc gctcggcgtg
      601 gtagcggaag gctacgtcgc ggaactccac gcgcgcaccg ctcaccgcca gggcgcgcgc
      661 gccggggcgg tcggcgacgc gcgggcgcac ggccagcagg ccgaacatct cctcgatcgc
      721 cgccagcgag cccttgatct cgcggtagac catgccgagg aaattgagcg gcatgaacag
      781 ctgcatcatg aaggcgttga tcagcacgaa gtcgcccacg ctcatctggg cgcggctcac
      841 gtcgcgcgcc gccagcgcca tgctcgcggt catcgccagc gccacgatca gcgcctgccc
      901 ggcattcagc gcgaacagcg tcaggcggtt cttgcgccgc gcctcctccc acctgccgag
      961 ttcggcgtcg tagcgcgcgg cctcgtgcgc ctcgttgccg aagtacttca ccgtctcgta
     1021 gttcagcagg ctgtcgacgg cgagcgtgtt gctggccgaa tccgccaggt tggccgcgcg
     1081 cacgtaggtc gtgcgccact cggtggcgac gatggagaac gcgatgtagg ccagcaccgc
     1141 ggccagcgtg atcgccgcgt aggcaatccc gtagttccac gccagcaggc ccgcgaccac
     1201 caggatctcg aacagcgttg gcacgatgtt gaacacgaag aagcgcatca ggaagctgat
     1261 gccgttggtg ccgcgctcga tgtcgcgcgc gagccccccg gtgcgccgct cgaggtggaa
     1321 gtccaggtcc agcccgtgca ggtgctcgaa gacgcgcagg cccacgcggc gcatcgcgcg
     1381 ctcggtgacg cgaccgaaca gcgtgtcgcg gatctcgccg aacatcacgt tggcgaaacg
     1441 cgctgcgccg taggcagcga ccagtcccag cgcggctttc agccatgccg cgccgccggc
     1501 gcccatcgta tccaccgcgt gcttgagcag gaagggcgcc cagatgctgc cgagcttcgc
     1561 gagcaccagg cagcccag
//
LOCUS       DAORUQ010000373         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_138237_length_1563_cov_4.467507, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000373 DAORUQ010000000
VERSION     DAORUQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138237_length_1563_cov_4.467507"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..>1563
                     /locus_tag="PLW13_16675"
     CDS             301..>1563
                     /locus_tag="PLW13_16675"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HQY72068.1"
                     /translation="MAESGDGSRKTSAVPGGRLSRLGRMARLATGIAGGMLAEGGRQI
                     AQGRMPRASELLLTPANARRLAGQLANLRGAAMKMGQLLSMDAGDLLPPQLSEILARL
                     RADAQAMPRRQLEGVLVKAWGKDWQRLFADFSWQPLAAASIGQVHRATTLDGRHLALK
                     IQYPGVARSIDSDVDNVATLLRIARLLPDGINLAPLLHEAKRQLKAEADYLKEAGHLR
                     HFGELLADDDDFVVPSVDEALTRREVLAMSYVPGHAVERMDVAAQAVRDRIAELLFRL
                     LFREMFEFRLVQTDPNFANFRYQDAGQRVALLDFGATRRYSKRVIDGYRTLFRGARDG
                     DRARLLAGAHEIGYFSADIEPRHREALLDLLELAGEPLAHPGSYDFGSSDLPARVRDA
                     GMVLSFEKGYWHEPPADAVFLHRKVGGVF"
BASE COUNT          229 a          514 c          580 g          240 t
ORIGIN      
        1 tgagcaggaa gggcgcccag atgctgccga gcttcgcgag caccaggcag cccagggcca
       61 gcgcgatgcg cgcgcggtat tgcgccaggt acggccagat catgcggatc gcgcgccagc
      121 tgaaccccgt ggcttcatgg cttgccgaat ggctgctgcg catggtcggg gggtgtctcc
      181 gggtgttcag ggtgccggcg cattctacac agcggtccgc gacagcgccg aacgcgtatg
      241 ctgtcgtggt ggatacacgg gcattcgcgc acaatgaggg cgcgggcaac ggaggccagg
      301 gtggctgaat cgggcgacgg gagcaggaag acatcggcgg tgccgggcgg caggctgtcg
      361 cggctggggc gcatggcgcg tctggcgacc ggcattgccg ggggcatgct ggccgagggc
      421 gggcgccaga tcgcgcaggg caggatgccg cgcgcgagcg agttgctgct gactcccgcg
      481 aatgcgcgcc ggctcgccgg gcaactcgcg aacctgcgcg gcgcggcgat gaagatgggg
      541 cagctgttgt cgatggacgc cggcgacctg ttgccgccgc agctctccga gatcctcgcg
      601 cggctgcgcg ccgacgcgca ggccatgccg cggcgccagc tcgagggcgt gctggtcaag
      661 gcctggggca aggactggca gcgcctgttc gccgacttct cgtggcaacc gctggcggcg
      721 gcctcgatcg gacaggtcca ccgcgccacc acgctcgacg ggcgccacct cgcgctgaag
      781 atccagtacc ccggtgtcgc gcgctccatc gacagcgatg tcgacaacgt cgcgacgctg
      841 ctgcgcatcg cgcggctgct gcccgatggc atcaatctcg cgccgctgct gcacgaggcc
      901 aagcgccagc tgaaggccga ggcggattac ctgaaggaag ccggacacct gcggcatttc
      961 ggcgagttgc tggccgacga cgacgatttc gtggtcccct ccgtcgacga ggcgctgacg
     1021 cgtcgcgagg tgctggcgat gagctatgtg ccggggcatg ccgtggagcg catggacgtg
     1081 gccgcgcagg cggtgcgcga ccgcatcgcg gagctgttgt tccggttgct gtttcgcgag
     1141 atgttcgagt tccgcctggt gcagaccgac ccgaatttcg ccaacttccg ctaccaggac
     1201 gcgggacagc gcgtcgccct gctggatttc ggggccacgc ggcgctactc gaaacgggtg
     1261 atcgacggct atcgcacgct gttccgcggc gcgcgcgacg gcgaccgcgc gcggctgctg
     1321 gccggcgcgc acgagatcgg gtatttttcc gccgacatcg agccgcgcca ccgcgaggca
     1381 ctgctcgacc tgctcgagct cgccggcgag cccctggcgc acccgggcag ttacgatttc
     1441 ggaagctcgg acctgccggc acgcgtgcgc gatgccggca tggtgctcag cttcgagaag
     1501 ggctactggc acgaaccgcc cgcggatgcg gtgttcctgc accgcaaggt cggcggcgtc
     1561 ttc
//
LOCUS       DAORUQ010000374         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_141032_length_1545_cov_7.534899, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000374 DAORUQ010000000
VERSION     DAORUQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141032_length_1545_cov_7.534899"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..>1545
                     /locus_tag="PLW13_16680"
     CDS             221..>1545
                     /locus_tag="PLW13_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQY72069.1"
                     /translation="MKTGRNDPCPCGSGRKYKQCCLARAGEARAAPKRDRGPGMALSW
                     LDQRFRRQTTTAIDGGFFAAFAPEEVRDALADLGPEYHEMLQINISDYLLSEAIYERG
                     DESGRGIDWVLESGPKLRPEQREFLEALADAPLRLYEVVSVRHGEGLELRDLLDPARS
                     PPFVTEHTASTTLRPGSVFGARILRLPDQHEIGGAVYPLDRPVAVALIARVGDELEGG
                     LDDPNEGDDDPDAPPMDLPPELAAAFARELEREDTPEELAGDAAFAAEIEDELLAAEI
                     RDTWLESLLFPQMPELVDRASGAPVLLIEDDYAVRDPAALAAALQACPDVVGDAEAGW
                     SRLDDPHAEVARPLTTINPGDAPGRVTLFHQTQARAEAGREWFERVAGTAVMRGERRT
                     IDPLAQLRHPPAIAPRTDPGPSIPHAKLNRLVELAIRRTYANWCDEPIPA"
BASE COUNT          242 a          569 c          521 g          213 t
ORIGIN      
        1 gaacccaccg ttcccgagac gcatgttccc actgcgcggc ggcgcgcgca ccccgcgtcg
       61 atgccccgcg tgcgatcggt gccccggcgg tgccggcgct cccgcaccgg gcagcgtgtc
      121 cgccaggtcg aatgccctgc cgaacctgcg gtcgagcccg ccttccccac tgcacggctg
      181 cccgctagag tccgcgcctg tcgaccgagg cagtccgtac atgaagacgg gacggaacga
      241 cccctgccct tgcggctcgg ggcgcaaata caagcaatgt tgcctcgcgc gcgcgggcga
      301 ggcgcgcgca gcccccaagc gcgatcgcgg cccgggcatg gcgctgtcct ggctggacca
      361 gcgcttccgg cgccagacca cgaccgcgat cgatggcggc ttcttcgccg ccttcgcacc
      421 ggaggaggtg cgcgacgcgc tcgccgatct cgggccggaa taccacgaga tgctgcagat
      481 caacatctcc gactatctct tgagcgaggc gatctacgag cgcggtgacg aaagcggccg
      541 cggcatcgat tgggtgctgg aatcgggccc gaagttgcgc ccggagcaac gcgaattcct
      601 cgaagcgctg gccgacgcac cgctgcggct gtacgaagtc gtgtccgttc gccacggcga
      661 gggcctggaa ctgcgcgacc tgctcgatcc cgcgcgcagc ccgccgttcg tgaccgagca
      721 cacggcttcg acgacattgc gacccggcag cgtgttcggc gcgcgcatcc tgcgcttgcc
      781 cgaccagcat gagatcggtg gcgccgttta cccgctcgat cgcccggtgg cggtcgcact
      841 gatcgcgcgg gttggcgacg agctcgaggg aggactcgac gaccccaacg agggcgacga
      901 cgatcccgac gccccgccga tggatcttcc gcccgaactg gccgcggcat tcgcccgcga
      961 gctggaacgc gaagacacgc cggaggagct ggccggcgat gcggccttcg ccgcggagat
     1021 cgaggacgag ctgctcgccg cggagatccg cgacacctgg ctcgagagcc tgctgttccc
     1081 gcagatgccg gaattggtgg accgtgccag cggcgcgccc gtgctgctca tcgaagacga
     1141 ctatgcggtg cgcgacccgg ccgcactggc cgcagcgctg caggcctgcc ccgatgtggt
     1201 cggcgatgcc gaggcgggct ggtcgcgatt ggacgacccg cacgccgagg ttgcacgccc
     1261 actcaccacg atcaatccgg gcgatgcacc gggccgcgtc accctcttcc accagaccca
     1321 ggccagggcc gaagcgggac gcgagtggtt cgagcgcgtc gcgggtacag cggtgatgcg
     1381 cggcgaacga cgcaccatcg acccgctcgc ccagctgcgc catccgcccg ccattgcgcc
     1441 gcgcaccgat ccgggccctt cgatcccgca cgccaagctg aatcgactgg tcgaactggc
     1501 gatccgccgc acctatgcca actggtgcga cgagccgatc ccggc
//
LOCUS       DAORUQ010000375         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_142802_length_1534_cov_4.959432, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000375 DAORUQ010000000
VERSION     DAORUQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142802_length_1534_cov_4.959432"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1534
                     /locus_tag="PLW13_16685"
     CDS             <1..>1534
                     /locus_tag="PLW13_16685"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQY72070.1"
                     /translation="LVERHEALRTVFVSEQGEPLQVVSKPQPWVLAVVDLGGEASPRE
                     RLKECLRAEAVRGFDLACGPLFRAQLYRLAPDTHVLLLAMHHIIYDGWSLGILYRELS
                     AFYGAFACGEAPTLPVLRVQYRDFARWQRGWLQGEVLESQLSYWRERLGGAPLVLELP
                     TDRERPPVESHRGAFHAFTLPRELVDRLQELSRREGATLFMALVSGFALLLARYSGQR
                     DLLIGTPVANRNRAEIEPLIGFFVNTLVLRADLSGEPSARAFLGRMREACMDAYAHQD
                     LPFERLVEELHPARDLSRNAVFQAMFALQNAPMEALQLRGLKLEPVEMEVGSAQFDLS
                     LYTWETPEGLAARFSYAADLFDGSTIARLAAHWRLLLEAMVATPDRRIDELPLLTVAE
                     RRQVLLEWNATARDYGQGKRLHGLVEAQAARTPEAVALEFEGRELTYAQLNGRANQLA
                     RVLRRKGVGPDALVGVFAERSFEMVVALLAVLKAGGAYLPLDPSYPVERLGHMLEDAR
                     APL"
BASE COUNT          203 a          461 c          590 g          280 t
ORIGIN      
        1 cctggtggag cgccacgagg cgctgcgcac ggtgttcgtg agcgagcagg gcgagccgct
       61 gcaggtcgtt tcgaagccgc agccgtgggt cctggcggtc gtggacttgg gcggggaggc
      121 aagccctcga gagcggctga aggagtgcct gcgcgcggag gccgtgcgcg ggtttgacct
      181 ggcgtgcggg ccgctattcc gcgcgcagct ctaccggctg gcgcccgaca cacacgtgct
      241 gctgctggcg atgcaccaca tcatctacga tggctggtcg cttggcatcc tgtaccggga
      301 gctaagcgcg ttctacgggg ctttcgcctg cggcgaggcg ccaactctgc cggtgctgcg
      361 cgtgcagtat cgtgactttg cgcgctggca gcgcggctgg ttgcagggcg aggtgctgga
      421 gtcgcagctt tcgtactggc gtgagcgcct gggcggggcg ccgctggtgc tggagttgcc
      481 caccgatcgg gaacgcccgc cggtggaaag ccatcgcggg gcgtttcatg cgttcacgct
      541 gcctcgcgag ttggttgatc gcctgcagga gttgtcccgg cgcgaggggg cgacgctatt
      601 catggcgctg gtctccggat tcgcgcttct gctcgcgcgc tacagcgggc agcgcgatct
      661 cctgatcggc acgccggtgg ccaaccgcaa ccgcgccgag atcgagccgc tgatcggatt
      721 cttcgtgaac acgctggtgc tgcgggccga tctttcgggc gagccttcgg cgcgggcgtt
      781 cctgggccgc atgcgtgagg cgtgcatgga cgcttacgct caccaggacc tgcccttcga
      841 gcggctggtg gaggagctgc accctgcgcg ggacctctcc cgcaatgcgg tgttccaggc
      901 gatgttcgcg ctgcaaaacg ccccgatgga ggcgctgcag ctgcgggggc tcaagctgga
      961 gcccgtcgag atggaagtcg ggtcggcaca gttcgatctc tcgctgtaca cgtgggaaac
     1021 cccggagggg ctcgctgccc gcttttccta cgcagccgat ctgttcgacg ggtcgacgat
     1081 cgcgcgcctg gctgcgcatt ggcgcttgtt gctcgaggcg atggtggcga cccccgatcg
     1141 ccgtatcgac gaattgccgc tgctcacggt tgccgagcgg cgccaggtgt tgctggagtg
     1201 gaatgccacg gcgcgggact atgggcaggg gaaacggctg cacgggctgg tcgaggcgca
     1261 ggcggcacgc acgccggaag ccgtcgcgct ggagttcgag gggcgggagc tgacatatgc
     1321 gcagttgaac ggcagggcga accagctcgc gcgtgtgctg cggcggaagg gcgtggggcc
     1381 ggatgcgctg gtgggagtgt ttgccgagcg ctccttcgag atggtggtgg cgctcctggc
     1441 ggtcttgaag gccgggggag cgtatttgcc gctggatccg tcatatccgg tcgagcgcct
     1501 gggccacatg ctggaggatg cgcgcgcgcc gctg
//
LOCUS       DAORUQ010000376         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_144686_length_1523_cov_6.523842, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000376 DAORUQ010000000
VERSION     DAORUQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144686_length_1523_cov_6.523842"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLW13_16690"
     CDS             complement(<1..1410)
                     /locus_tag="PLW13_16690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY72071.1"
                     /translation="MNMPAQIDAHAPSAAELAGQPRVVIIGAGMSGMLMAIRLKQAGN
                     TNFRIYEKAAKLGGTWRENTYPNIACDVYAPAYTYSFEPNTEWNWRFGRGEEIQRYFE
                     RVGDKYGLHEYISFGCEVSEARWNGAQWEIRLGNGETDRADVLVSAVGALHHPRYPDI
                     EGLGSFSGPCFHTARWDHTVDLRGKRVGIIGTGSTAAQVVPGIVDKVEQLYLFQRTPQ
                     WVLPAPDRKFSERYRQRRRKHRWLARFDHWFNLQLFQLFGRAVVGNRLLMRGIEYACR
                     ANLKTIRDPELRRKLTPDYQVACKRVVVSEDFYPAIQKENAHLVTEGIARIVPEGVQL
                     KDGTVVALDVLVLSTGFYPYKTTVEVYGENGGSLREAWGGSPLMYRTIGVPGFPNYFV
                     LFGPYSPIGNMSIIENSEIQAGYVMQCIELIRRGAVKTLNPKESVARALKEEMRAGLK
                     KTAWAGGCSSWYLDANGELP"
BASE COUNT          240 a          515 c          503 g          265 t
ORIGIN      
        1 cggcagctcg ccgttggcgt cgaggtacca gctgctgcag ccaccggccc aggcggtctt
       61 cttcaggccc gcgcgcatct cctccttcag cgcacgcgcc accgattcct tcgggttcag
      121 ggtcttcacc gcaccgcggc ggatcagctc gatgcactgc atgacgtacc cggcctggat
      181 ctcggagttc tcgatgatcg acatgttgcc gatcgggctg tagggcccga acagcacgaa
      241 gtagttcggg aaacccggca ccccgatggt gcggtacatc agcggactgc cgccccaggc
      301 ctcgcgcagg ctgccgccgt tctctccgta cacctcaacc gtggtcttgt aggggtaaaa
      361 gccggtcgag agcaccagca cgtcgagcgc gaccaccgtg ccgtccttca attgcacgcc
      421 ctcgggcacg atacgcgcga tgccctcggt cacgaggtgc gcgttctcct tctggatcgc
      481 cggatagaag tcctccgaga ccaccacgcg cttgcaggcg acctggtagt cgggcgtgag
      541 cttgcggcgc agctccgggt cgcggatcgt cttcaggttg gcgcggcagg cgtactcgat
      601 gccgcgcatc agcaagcggt tgcccaccac cgcgcgcccg aacaactgga acaactgcag
      661 gttgaaccag tggtcgaagc gcgccagcca gcggtgcttg cgccggcgct gccggtagcg
      721 ctcgctgaac ttgcgatccg gcgccggcag cacccactgc ggcgtgcgct ggaacaggta
      781 gagctgttcg accttgtcga caatgcccgg caccacctgc gccgccgtgg agccggtgcc
      841 gatgatgccc acgcgcttgc cgcgcaggtc caccgtgtgg tcccagcgcg ccgtgtggaa
      901 gcacggaccg gagaagctcc cgagcccctc gatgtcgggg tagcgcgggt gatgcagcgc
      961 gccgaccgcg gagaccagca cgtcggcgcg atccgtttca ccgttgccga ggcggatctc
     1021 ccactgcgcg ccgttccagc gcgcctcgct gacctcgcag ccgaaactga tgtactcgtg
     1081 cagcccgtac ttgtcgccga cccgctcgaa gtagcgctgg atctcctcgc cgcgaccgaa
     1141 gcgccagttc cactcggtat tgggctcgaa gctgtaggta taggccggcg cgtacacatc
     1201 gcaggcgata ttggggtagg tgttctcgcg ccaggtgccg ccgagcttcg cggccttctc
     1261 gtagatgcgg aaattggtgt tgcccgcctg cttcagccgg atcgccatca gcatccccga
     1321 catgccggcg ccgatgatca ccacgcgcgg ctggcccgcg agctcggcgg ctgagggggc
     1381 gtgggcgtcg atctgtgccg gcatgttcat cggggttagg ctccaggatg ggctgcgtcg
     1441 attggcgagc gggacattat tacgaaccag cgcggcaggg aagccgcgac gggttacgat
     1501 ccgatgacgg ttggccaggc tcg
//
LOCUS       DAORUQ010000377         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_5261
            NODE_144688_length_1523_cov_6.354223, whole genome shotgun
            sequence.
ACCESSION   DAORUQ010000377 DAORUQ010000000
VERSION     DAORUQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867478
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:55:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,340
            CDSs (total)                      :: 3,316
            Genes (coding)                    :: 3,298
            CDSs (with protein)               :: 3,298
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144688_length_1523_cov_6.354223"
                     /isolate="AMR_MDS_5261"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="ftsZ"
                     /locus_tag="PLW13_16695"
     CDS             complement(<1..947)
                     /gene="ftsZ"
                     /locus_tag="PLW13_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQY72072.1"
                     /translation="MFELVDNVAQSAVIKVVGVGGGGGNAVKHMIGGAVEGVEFICAN
                     TDAQALSDISSRTVLQLGSGITKGLGAGANPEIGRQAALEDRDRIAEVLSGADMVFIT
                     AGMGGGTGTGAAPIVAEVARELGILTVAVVTRPFKFEGKKRASIADEGLMELQQHVDS
                     LITIPNEKLLSVLGKNTSLLDAFKAANDVLLGAVQGIADLIIRPGMINVDFADVRTVM
                     SEMGMAMMGTGVGRGENRAREAAEAAIRSPLLEDINLQGARGILVNITAGPDLSLGEF
                     EEVGDTVEEFASDTATVVVGTVIDPEMNGEIKVTVVATGL"
     gene            complement(987..>1523)
                     /gene="ftsA"
                     /locus_tag="PLW13_16700"
     CDS             complement(987..>1523)
                     /gene="ftsA"
                     /locus_tag="PLW13_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQY72073.1"
                     /translation="IPIAGDQVTNDIAMALRTPTQYAEEIKIRYACALAQLAGADQTI
                     KVPGVGERGARDLSRQALAEVVEPRYDELFTLVQAEIRRSGFEDVLAGGIVLTGGTAK
                     MEGVVELAEEIFHMPVRIGAPAGVQGMLDIVKNPIYSTAVGLLLFGVKQYTANAGVKE
                     SPGRGLVKKMKQWFQGNF"
BASE COUNT          235 a          546 c          472 g          270 t
ORIGIN      
        1 aggccggtgg cgaccaccgt gaccttgatc tcgccgttca tttccgggtc gatcacggtg
       61 ccgaccacga cggtcgccgt gtcggaagcg aactcctcga cggtgtcacc aacctcctcg
      121 aactcgccga gcgagaggtc gggacccgcg gtgatgttca ccaggatgcc gcgcgcgccc
      181 tgcaggttga tgtcctcgag cagcgggctg cggatggccg cctcggcggc ctcgcgcgcg
      241 cggttctcgc cgcggcccac accggtgccc atcatggcca tgcccatttc ggacatcacg
      301 gtgcgcacgt cggcgaagtc gacgttgatc atgcccggac ggatgatcag gtcggcgatg
      361 ccctgcaccg cgcccagcag cacgtcgttg gctgccttga acgcatccag caggctggtg
      421 ttcttgccca gcacgctcag cagcttctcg ttcgggatcg tgatcagcga gtcgacgtgc
      481 tgctgcagct ccatcaggcc ctcgtcggcg atcgaggcgc gcttcttgcc ttcgaacttg
      541 aaggggcgcg tgaccacggc gaccgtgagt atgcccaact cccgcgccac ttccgccacg
      601 atcggcgctg ccccggtgcc ggtacccccg cccatgccgg cggtgatgaa caccatgtcc
      661 gcgcccgaga gcacctcggc gatgcggtca cggtcctcga gcgccgcctg gcggccgatc
      721 tccgggttcg cgcccgcgcc caggcccttg gtgatgcccg agccgagctg cagcacggtg
      781 cgcgaggaaa tgtctgaaag tgcctgcgca tcggtgttgg cgcagatgaa ctccacgccc
      841 tcgaccgcgc cgccgatcat gtgcttcacg gcgttgccgc cgcctccgcc cacgccgacg
      901 accttgatca ctgcgctctg cgctacgttg tctacgagtt cgaacatggc tatcaccctc
      961 cgaggttgaa cgatcaatcg aaaaaatcag aaattgccct ggaaccactg cttcatcttc
     1021 ttcacgagac cccgccccgg ggactccttg acccccgcgt tggccgtgta ctgcttgacg
     1081 ccgaacagca gcagcccgac ggcggtggaa taaatggggt tcttcacgat atcgagcatg
     1141 ccctgcacac cggcgggcgc ccctatgcgc accggcatgt ggaatatctc ctcggccaac
     1201 tccacgacgc cctccatctt cgcggtgccg ccggtcagca cgatgccgcc ggccagcacg
     1261 tcctcgaagc cgctgcggcg gatctcggcc tgcaccagcg tgaacagctc gtcgtaacgc
     1321 ggctcgacca cttcggccag cgcctggcgc gagaggtcgc gcgcgccgcg ctccccgacg
     1381 ccggggacct tgatcgtctg gtccgcgccg gcgagctgcg ccaatgcgca ggcgtagcgg
     1441 atcttgattt cctcggcata ctgcgtcggc gtgcgcagcg ccatcgcgat gtcgttggtg
     1501 acctggtcgc cggcgatcgg gat
//
